LOCUS       DAFATA010000001       194701 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFATA010000001 DAFATA010000000
VERSION     DAFATA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 194701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 194701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..194701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.001"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="LGJ20_000001"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="LGJ20_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBK2384137.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="LGJ20_000002"
     CDS             complement(1608..1931)
                     /locus_tag="LGJ20_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBK2384138.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="LGJ20_000003"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="LGJ20_000003"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBK2384139.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="LGJ20_000004"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="LGJ20_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBK2384140.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="LGJ20_000005"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="LGJ20_000005"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBK2384141.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="LGJ20_000006"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="LGJ20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBK2384142.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="LGJ20_000007"
     CDS             7531..8568
                     /locus_tag="LGJ20_000007"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBK2384143.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSYIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="LGJ20_000008"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="LGJ20_000008"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK2384144.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="LGJ20_000009"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="LGJ20_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBK2384145.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10048..10635)
                     /gene="eamB"
                     /locus_tag="LGJ20_000010"
     CDS             complement(10048..10635)
                     /gene="eamB"
                     /locus_tag="LGJ20_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBK2384146.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            10738..11619
                     /locus_tag="LGJ20_000011"
     CDS             10738..11619
                     /locus_tag="LGJ20_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2384147.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11828..13162)
                     /gene="srmB"
                     /locus_tag="LGJ20_000012"
     CDS             complement(11828..13162)
                     /gene="srmB"
                     /locus_tag="LGJ20_000012"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBK2384148.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13294..14031
                     /gene="trmN"
                     /locus_tag="LGJ20_000013"
     CDS             13294..14031
                     /gene="trmN"
                     /locus_tag="LGJ20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBK2384149.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14016..15638)
                     /gene="nadB"
                     /locus_tag="LGJ20_000014"
     CDS             complement(14016..15638)
                     /gene="nadB"
                     /locus_tag="LGJ20_000014"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBK2384150.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLELNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15894..16049
                     /gene="rseD"
                     /locus_tag="LGJ20_000015"
     CDS             15894..16049
                     /gene="rseD"
                     /locus_tag="LGJ20_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBK2384151.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16046..16621
                     /gene="rpoE"
                     /locus_tag="LGJ20_000016"
     CDS             16046..16621
                     /gene="rpoE"
                     /locus_tag="LGJ20_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBK2384152.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16654..17304
                     /gene="rseA"
                     /locus_tag="LGJ20_000017"
     CDS             16654..17304
                     /gene="rseA"
                     /locus_tag="LGJ20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBK2384153.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17304..18260
                     /gene="rseB"
                     /locus_tag="LGJ20_000018"
     CDS             17304..18260
                     /gene="rseB"
                     /locus_tag="LGJ20_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBK2384154.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18257..18736
                     /gene="rseC"
                     /locus_tag="LGJ20_000019"
     CDS             18257..18736
                     /gene="rseC"
                     /locus_tag="LGJ20_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBK2384155.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18934..20733
                     /gene="lepA"
                     /locus_tag="LGJ20_000020"
     CDS             18934..20733
                     /gene="lepA"
                     /locus_tag="LGJ20_000020"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK2384156.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20749..21723
                     /gene="lepB"
                     /locus_tag="LGJ20_000021"
     CDS             20749..21723
                     /gene="lepB"
                     /locus_tag="LGJ20_000021"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK2384157.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21996..22676
                     /gene="rnc"
                     /locus_tag="LGJ20_000022"
     CDS             21996..22676
                     /gene="rnc"
                     /locus_tag="LGJ20_000022"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK2384158.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22673..23578
                     /gene="era"
                     /locus_tag="LGJ20_000023"
     CDS             22673..23578
                     /gene="era"
                     /locus_tag="LGJ20_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK2384159.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23590..24318
                     /gene="recO"
                     /locus_tag="LGJ20_000024"
     CDS             23590..24318
                     /gene="recO"
                     /locus_tag="LGJ20_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK2384160.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24330..25061
                     /gene="pdxJ"
                     /locus_tag="LGJ20_000025"
     CDS             24330..25061
                     /gene="pdxJ"
                     /locus_tag="LGJ20_000025"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBK2384161.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25061..25441
                     /locus_tag="LGJ20_000026"
     CDS             25061..25441
                     /locus_tag="LGJ20_000026"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK2384162.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25861..25941
                     /gene="shoB"
                     /locus_tag="LGJ20_000027"
     CDS             25861..25941
                     /gene="shoB"
                     /locus_tag="LGJ20_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBK2384163.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(26135..26395)
                     /gene="yfhL"
                     /locus_tag="LGJ20_000028"
     CDS             complement(26135..26395)
                     /gene="yfhL"
                     /locus_tag="LGJ20_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBK2384164.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26451..27299)
                     /locus_tag="LGJ20_000029"
     CDS             complement(26451..27299)
                     /locus_tag="LGJ20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK2384165.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27508..28143
                     /gene="yfhb"
                     /locus_tag="LGJ20_000030"
     CDS             27508..28143
                     /gene="yfhb"
                     /locus_tag="LGJ20_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBK2384166.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28168..28704
                     /gene="tadA"
                     /locus_tag="LGJ20_000031"
     CDS             28168..28704
                     /gene="tadA"
                     /locus_tag="LGJ20_000031"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK2384167.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(28701..30257)
                     /gene="mltF"
                     /locus_tag="LGJ20_000032"
     CDS             complement(28701..30257)
                     /gene="mltF"
                     /locus_tag="LGJ20_000032"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBK2384168.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30515..34402
                     /gene="purL"
                     /locus_tag="LGJ20_000033"
                     /gene_synonym="purI"
     CDS             30515..34402
                     /gene="purL"
                     /locus_tag="LGJ20_000033"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBK2384169.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34978..36405
                     /gene="qseE"
                     /locus_tag="LGJ20_000034"
     CDS             34978..36405
                     /gene="qseE"
                     /locus_tag="LGJ20_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBK2384170.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36570..37283
                     /gene="qseG"
                     /locus_tag="LGJ20_000035"
     CDS             36570..37283
                     /gene="qseG"
                     /locus_tag="LGJ20_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBK2384171.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            37273..38607
                     /gene="glrR"
                     /locus_tag="LGJ20_000036"
     CDS             37273..38607
                     /gene="glrR"
                     /locus_tag="LGJ20_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBK2384172.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38668..39006
                     /gene="glnB"
                     /locus_tag="LGJ20_000037"
     CDS             38668..39006
                     /gene="glnB"
                     /locus_tag="LGJ20_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBK2384173.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(39051..40241)
                     /gene="hmpA"
                     /locus_tag="LGJ20_000038"
     CDS             complement(39051..40241)
                     /gene="hmpA"
                     /locus_tag="LGJ20_000038"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBK2384174.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40569..41822
                     /gene="glyA"
                     /locus_tag="LGJ20_000039"
     CDS             40569..41822
                     /gene="glyA"
                     /locus_tag="LGJ20_000039"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK2384175.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41886..43079)
                     /locus_tag="LGJ20_000040"
     CDS             complement(41886..43079)
                     /locus_tag="LGJ20_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK2384176.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43197..46478
                     /locus_tag="LGJ20_000041"
     CDS             43197..46478
                     /locus_tag="LGJ20_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBK2384177.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     ATEMLNALDCQRTLSLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            46575..47558
                     /locus_tag="LGJ20_000042"
     CDS             46575..47558
                     /locus_tag="LGJ20_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK2384178.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            47581..49092
                     /locus_tag="LGJ20_000043"
     CDS             47581..49092
                     /locus_tag="LGJ20_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK2384179.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            49117..50115
                     /locus_tag="LGJ20_000044"
     CDS             49117..50115
                     /locus_tag="LGJ20_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2384180.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            50148..51242
                     /locus_tag="LGJ20_000045"
     CDS             50148..51242
                     /locus_tag="LGJ20_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBK2384181.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            51254..52126
                     /locus_tag="LGJ20_000046"
     CDS             51254..52126
                     /locus_tag="LGJ20_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBK2384182.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLMVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(52174..52596)
                     /locus_tag="LGJ20_000047"
     CDS             complement(52174..52596)
                     /locus_tag="LGJ20_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBK2384183.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNLINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(52693..53895)
                     /locus_tag="LGJ20_000048"
     CDS             complement(52693..53895)
                     /locus_tag="LGJ20_000048"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBK2384184.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(53905..54717)
                     /gene="hcaB"
                     /locus_tag="LGJ20_000049"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(53905..54717)
                     /gene="hcaB"
                     /locus_tag="LGJ20_000049"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBK2384185.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(54714..55034)
                     /locus_tag="LGJ20_000050"
     CDS             complement(54714..55034)
                     /locus_tag="LGJ20_000050"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBK2384186.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(55034..55552)
                     /locus_tag="LGJ20_000051"
     CDS             complement(55034..55552)
                     /locus_tag="LGJ20_000051"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBK2384187.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(55549..56910)
                     /locus_tag="LGJ20_000052"
     CDS             complement(55549..56910)
                     /locus_tag="LGJ20_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBK2384188.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            57046..57936
                     /gene="hcaR"
                     /locus_tag="LGJ20_000053"
     CDS             57046..57936
                     /gene="hcaR"
                     /locus_tag="LGJ20_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBK2384189.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            58096..59235
                     /locus_tag="LGJ20_000054"
     CDS             58096..59235
                     /locus_tag="LGJ20_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBK2384190.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(59227..60507)
                     /gene="csiE"
                     /locus_tag="LGJ20_000055"
     CDS             complement(59227..60507)
                     /gene="csiE"
                     /locus_tag="LGJ20_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBK2384191.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(60698..61552)
                     /locus_tag="LGJ20_000056"
     CDS             complement(60698..61552)
                     /locus_tag="LGJ20_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2384192.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(61697..62500)
                     /gene="suhB"
                     /locus_tag="LGJ20_000057"
     CDS             complement(61697..62500)
                     /gene="suhB"
                     /locus_tag="LGJ20_000057"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBK2384193.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            62619..63356
                     /gene="trmJ"
                     /locus_tag="LGJ20_000058"
     CDS             62619..63356
                     /gene="trmJ"
                     /locus_tag="LGJ20_000058"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBK2384194.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            63657..64145
                     /gene="iscR"
                     /locus_tag="LGJ20_000059"
     CDS             63657..64145
                     /gene="iscR"
                     /locus_tag="LGJ20_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBK2384195.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            64257..65471
                     /gene="iscS"
                     /locus_tag="LGJ20_000060"
     CDS             64257..65471
                     /gene="iscS"
                     /locus_tag="LGJ20_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK2384196.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            65499..65885
                     /gene="iscU"
                     /locus_tag="LGJ20_000061"
     CDS             65499..65885
                     /gene="iscU"
                     /locus_tag="LGJ20_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBK2384197.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            65902..66225
                     /gene="iscA"
                     /locus_tag="LGJ20_000062"
     CDS             65902..66225
                     /gene="iscA"
                     /locus_tag="LGJ20_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBK2384198.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            66321..66836
                     /gene="hscB"
                     /locus_tag="LGJ20_000063"
     CDS             66321..66836
                     /gene="hscB"
                     /locus_tag="LGJ20_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBK2384199.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            66853..68703
                     /gene="hscA"
                     /locus_tag="LGJ20_000064"
     CDS             66853..68703
                     /gene="hscA"
                     /locus_tag="LGJ20_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBK2384200.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            68705..69040
                     /gene="fdx"
                     /locus_tag="LGJ20_000065"
     CDS             68705..69040
                     /gene="fdx"
                     /locus_tag="LGJ20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBK2384201.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            69052..69252
                     /gene="iscX"
                     /locus_tag="LGJ20_000066"
     CDS             69052..69252
                     /gene="iscX"
                     /locus_tag="LGJ20_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBK2384202.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            69430..70713
                     /gene="pepB"
                     /locus_tag="LGJ20_000067"
     CDS             69430..70713
                     /gene="pepB"
                     /locus_tag="LGJ20_000067"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBK2384203.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            70855..71631
                     /gene="sseB"
                     /locus_tag="LGJ20_000068"
     CDS             70855..71631
                     /gene="sseB"
                     /locus_tag="LGJ20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBK2384204.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(72124..72969)
                     /gene="sseA"
                     /locus_tag="LGJ20_000069"
     CDS             complement(72124..72969)
                     /gene="sseA"
                     /locus_tag="LGJ20_000069"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBK2384205.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            73176..78137
                     /gene="yfhM"
                     /locus_tag="LGJ20_000070"
     CDS             73176..78137
                     /gene="yfhM"
                     /locus_tag="LGJ20_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBK2384206.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            78138..80450
                     /gene="pbpC"
                     /locus_tag="LGJ20_000071"
     CDS             78138..80450
                     /gene="pbpC"
                     /locus_tag="LGJ20_000071"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBK2384207.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            80599..81030
                     /gene="ndk"
                     /locus_tag="LGJ20_000072"
     CDS             80599..81030
                     /gene="ndk"
                     /locus_tag="LGJ20_000072"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK2384208.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            81180..82334
                     /gene="trmG/rlmN"
                     /locus_tag="LGJ20_000073"
     CDS             81180..82334
                     /gene="trmG/rlmN"
                     /locus_tag="LGJ20_000073"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBK2384209.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            82619..83632
                     /gene="rodZ"
                     /locus_tag="LGJ20_000074"
     CDS             82619..83632
                     /gene="rodZ"
                     /locus_tag="LGJ20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBK2384210.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            83659..84777
                     /gene="ispG"
                     /locus_tag="LGJ20_000075"
                     /gene_synonym="gcpE"
     CDS             83659..84777
                     /gene="ispG"
                     /locus_tag="LGJ20_000075"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBK2384211.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            84888..86162
                     /gene="hisS"
                     /locus_tag="LGJ20_000076"
     CDS             84888..86162
                     /gene="hisS"
                     /locus_tag="LGJ20_000076"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK2384212.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQYKDKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            86180..86800
                     /gene="yfgM"
                     /locus_tag="LGJ20_000077"
     CDS             86180..86800
                     /gene="yfgM"
                     /locus_tag="LGJ20_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBK2384213.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            86811..87989
                     /gene="bamB"
                     /locus_tag="LGJ20_000078"
     CDS             86811..87989
                     /gene="bamB"
                     /locus_tag="LGJ20_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBK2384214.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            88107..89579
                     /gene="der"
                     /locus_tag="LGJ20_000079"
     CDS             88107..89579
                     /gene="der"
                     /locus_tag="LGJ20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK2384215.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            89648..89863
                     /locus_tag="LGJ20_000080"
     CDS             89648..89863
                     /locus_tag="LGJ20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK2384216.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(89860..91230)
                     /gene="xseA"
                     /locus_tag="LGJ20_000081"
     CDS             complement(89860..91230)
                     /gene="xseA"
                     /locus_tag="LGJ20_000081"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK2384217.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            91392..92858
                     /gene="guaB"
                     /locus_tag="LGJ20_000082"
     CDS             91392..92858
                     /gene="guaB"
                     /locus_tag="LGJ20_000082"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK2384218.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            92927..94504
                     /gene="guaA"
                     /locus_tag="LGJ20_000083"
     CDS             92927..94504
                     /gene="guaA"
                     /locus_tag="LGJ20_000083"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK2384219.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(94597..95136)
                     /locus_tag="LGJ20_000084"
     CDS             complement(94597..95136)
                     /locus_tag="LGJ20_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBK2384220.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSESWFN"
     gene            complement(95152..95670)
                     /locus_tag="LGJ20_000085"
     CDS             complement(95152..95670)
                     /locus_tag="LGJ20_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBK2384221.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(95981..96172)
                     /locus_tag="LGJ20_000086"
     CDS             complement(95981..96172)
                     /locus_tag="LGJ20_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBK2384222.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            96190..96342
                     /locus_tag="LGJ20_000087"
     CDS             96190..96342
                     /locus_tag="LGJ20_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384223.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            96524..98767
                     /gene="pdeF"
                     /locus_tag="LGJ20_000088"
     CDS             96524..98767
                     /gene="pdeF"
                     /locus_tag="LGJ20_000088"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBK2384224.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(98806..100347)
                     /gene="ppx"
                     /locus_tag="LGJ20_000089"
     CDS             complement(98806..100347)
                     /gene="ppx"
                     /locus_tag="LGJ20_000089"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBK2384225.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(100352..102418)
                     /gene="ppk1"
                     /locus_tag="LGJ20_000090"
     CDS             complement(100352..102418)
                     /gene="ppk1"
                     /locus_tag="LGJ20_000090"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBK2384226.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(102589..103227)
                     /gene="purN"
                     /locus_tag="LGJ20_000091"
     CDS             complement(102589..103227)
                     /gene="purN"
                     /locus_tag="LGJ20_000091"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK2384227.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(103227..104264)
                     /gene="purM"
                     /locus_tag="LGJ20_000092"
     CDS             complement(103227..104264)
                     /gene="purM"
                     /locus_tag="LGJ20_000092"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK2384228.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            104588..105214
                     /gene="upp"
                     /locus_tag="LGJ20_000093"
     CDS             104588..105214
                     /gene="upp"
                     /locus_tag="LGJ20_000093"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK2384229.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            105300..106589
                     /gene="uraA"
                     /locus_tag="LGJ20_000094"
     CDS             105300..106589
                     /gene="uraA"
                     /locus_tag="LGJ20_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK2384230.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            106639..107385
                     /gene="hda"
                     /locus_tag="LGJ20_000095"
     CDS             106639..107385
                     /gene="hda"
                     /locus_tag="LGJ20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBK2384231.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(107523..107882)
                     /gene="arsC"
                     /locus_tag="LGJ20_000096"
     CDS             complement(107523..107882)
                     /gene="arsC"
                     /locus_tag="LGJ20_000096"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBK2384232.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(107903..109366)
                     /gene="bepA"
                     /locus_tag="LGJ20_000097"
     CDS             complement(107903..109366)
                     /gene="bepA"
                     /locus_tag="LGJ20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBK2384233.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            109579..110640
                     /locus_tag="LGJ20_000098"
     CDS             109579..110640
                     /locus_tag="LGJ20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK2384234.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(110678..111525)
                     /locus_tag="LGJ20_000099"
                     /pseudo
     CDS             complement(110678..111525)
                     /locus_tag="LGJ20_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(111547..113559)
                     /gene="hyfR"
                     /locus_tag="LGJ20_000100"
                     /pseudo
     CDS             complement(111547..113559)
                     /gene="hyfR"
                     /locus_tag="LGJ20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(113589..114002)
                     /gene="hyfJ"
                     /locus_tag="LGJ20_000101"
     CDS             complement(113589..114002)
                     /gene="hyfJ"
                     /locus_tag="LGJ20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBK2384235.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(113995..114752)
                     /gene="hyfI"
                     /locus_tag="LGJ20_000102"
                     /pseudo
     CDS             complement(113995..114752)
                     /gene="hyfI"
                     /locus_tag="LGJ20_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(114749..115294)
                     /gene="hyfH"
                     /locus_tag="LGJ20_000103"
     CDS             complement(114749..115294)
                     /gene="hyfH"
                     /locus_tag="LGJ20_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBK2384236.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(115304..116971)
                     /gene="hyfG"
                     /locus_tag="LGJ20_000104"
     CDS             complement(115304..116971)
                     /gene="hyfG"
                     /locus_tag="LGJ20_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBK2384237.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(116961..118541)
                     /gene="hyfF"
                     /locus_tag="LGJ20_000105"
     CDS             complement(116961..118541)
                     /gene="hyfF"
                     /locus_tag="LGJ20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBK2384238.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(118546..119196)
                     /gene="hyfE"
                     /locus_tag="LGJ20_000106"
     CDS             complement(118546..119196)
                     /gene="hyfE"
                     /locus_tag="LGJ20_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBK2384239.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(119208..120439)
                     /locus_tag="LGJ20_000107"
                     /pseudo
     CDS             complement(119208..120439)
                     /locus_tag="LGJ20_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(120456..121403)
                     /gene="hyfC"
                     /locus_tag="LGJ20_000108"
     CDS             complement(120456..121403)
                     /gene="hyfC"
                     /locus_tag="LGJ20_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBK2384240.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(121414..123432)
                     /gene="hyfB"
                     /locus_tag="LGJ20_000109"
     CDS             complement(121414..123432)
                     /gene="hyfB"
                     /locus_tag="LGJ20_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HBK2384241.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVAHFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMLLSSRFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(123432..124049)
                     /gene="hyfA"
                     /locus_tag="LGJ20_000110"
     CDS             complement(123432..124049)
                     /gene="hyfA"
                     /locus_tag="LGJ20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBK2384242.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(124302..124772)
                     /gene="bcp"
                     /locus_tag="LGJ20_000111"
     CDS             complement(124302..124772)
                     /gene="bcp"
                     /locus_tag="LGJ20_000111"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBK2384243.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(124772..125344)
                     /locus_tag="LGJ20_000112"
     CDS             complement(124772..125344)
                     /locus_tag="LGJ20_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBK2384244.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            125490..126368
                     /gene="dapA"
                     /locus_tag="LGJ20_000113"
     CDS             125490..126368
                     /gene="dapA"
                     /locus_tag="LGJ20_000113"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK2384245.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            126385..127419
                     /gene="bamC"
                     /locus_tag="LGJ20_000114"
     CDS             126385..127419
                     /gene="bamC"
                     /locus_tag="LGJ20_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBK2384246.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            127632..128345
                     /gene="purC"
                     /locus_tag="LGJ20_000115"
     CDS             127632..128345
                     /gene="purC"
                     /locus_tag="LGJ20_000115"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK2384247.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            128499..128726
                     /gene="vapB"
                     /locus_tag="LGJ20_000116"
     CDS             128499..128726
                     /gene="vapB"
                     /locus_tag="LGJ20_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBK2384248.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            128726..129124
                     /gene="vapC"
                     /locus_tag="LGJ20_000117"
     CDS             128726..129124
                     /gene="vapC"
                     /locus_tag="LGJ20_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBK2384249.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            129271..130134
                     /locus_tag="LGJ20_000118"
     CDS             129271..130134
                     /locus_tag="LGJ20_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBK2384250.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            130149..132164
                     /gene="tmcA"
                     /locus_tag="LGJ20_000119"
     CDS             130149..132164
                     /gene="tmcA"
                     /locus_tag="LGJ20_000119"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBK2384251.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALCALNDVRTERLRDRITQWQFFH"
     gene            132238..132936
                     /gene="ypfH"
                     /locus_tag="LGJ20_000120"
     CDS             132238..132936
                     /gene="ypfH"
                     /locus_tag="LGJ20_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK2384252.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(133046..133246)
                     /locus_tag="LGJ20_000121"
     CDS             complement(133046..133246)
                     /locus_tag="LGJ20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBK2384253.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(133274..134401)
                     /gene="dapE"
                     /locus_tag="LGJ20_000122"
     CDS             complement(133274..134401)
                     /gene="dapE"
                     /locus_tag="LGJ20_000122"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBK2384254.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(134405..134761)
                     /locus_tag="LGJ20_000123"
     CDS             complement(134405..134761)
                     /locus_tag="LGJ20_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBK2384255.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            135142..135201
                     /gene="ypfM"
                     /locus_tag="LGJ20_000124"
     CDS             135142..135201
                     /gene="ypfM"
                     /locus_tag="LGJ20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBK2384256.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(135300..138413)
                     /gene="acrD"
                     /locus_tag="LGJ20_000125"
     CDS             complement(135300..138413)
                     /gene="acrD"
                     /locus_tag="LGJ20_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBK2384257.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(138577..140277)
                     /gene="narQ"
                     /locus_tag="LGJ20_000126"
     CDS             complement(138577..140277)
                     /gene="narQ"
                     /locus_tag="LGJ20_000126"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBK2384258.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            140483..142462
                     /gene="aegA"
                     /locus_tag="LGJ20_000127"
     CDS             140483..142462
                     /gene="aegA"
                     /locus_tag="LGJ20_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBK2384259.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            142530..143105
                     /gene="nudK"
                     /locus_tag="LGJ20_000128"
     CDS             142530..143105
                     /gene="nudK"
                     /locus_tag="LGJ20_000128"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBK2384260.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            143231..144274
                     /locus_tag="LGJ20_000129"
     CDS             143231..144274
                     /locus_tag="LGJ20_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBK2384261.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNVLLDYGNWRMNGLRCSL
                     DPLRREANVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(144370..146373)
                     /gene="tkt"
                     /locus_tag="LGJ20_000130"
     CDS             complement(144370..146373)
                     /gene="tkt"
                     /locus_tag="LGJ20_000130"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK2384262.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTAGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(146393..147343)
                     /gene="tal"
                     /locus_tag="LGJ20_000131"
     CDS             complement(146393..147343)
                     /gene="tal"
                     /locus_tag="LGJ20_000131"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBK2384263.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            147632..149911
                     /gene="maeB"
                     /locus_tag="LGJ20_000132"
     CDS             147632..149911
                     /gene="maeB"
                     /locus_tag="LGJ20_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBK2384264.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            150204..150539
                     /gene="eutS"
                     /locus_tag="LGJ20_000133"
     CDS             150204..150539
                     /gene="eutS"
                     /locus_tag="LGJ20_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBK2384265.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            150552..151031
                     /gene="eutP"
                     /locus_tag="LGJ20_000134"
     CDS             150552..151031
                     /gene="eutP"
                     /locus_tag="LGJ20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBK2384266.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            151006..151707
                     /gene="eutQ"
                     /locus_tag="LGJ20_000135"
     CDS             151006..151707
                     /gene="eutQ"
                     /locus_tag="LGJ20_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBK2384267.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            151704..152507
                     /gene="eutT"
                     /locus_tag="LGJ20_000136"
     CDS             151704..152507
                     /gene="eutT"
                     /locus_tag="LGJ20_000136"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBK2384268.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            152504..153520
                     /gene="pta"
                     /locus_tag="LGJ20_000137"
     CDS             152504..153520
                     /gene="pta"
                     /locus_tag="LGJ20_000137"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK2384269.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            153559..153852
                     /gene="eutM"
                     /locus_tag="LGJ20_000138"
     CDS             153559..153852
                     /gene="eutM"
                     /locus_tag="LGJ20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBK2384270.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            153959..154246
                     /gene="eutN"
                     /locus_tag="LGJ20_000139"
     CDS             153959..154246
                     /gene="eutN"
                     /locus_tag="LGJ20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBK2384271.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            154258..155661
                     /locus_tag="LGJ20_000140"
     CDS             154258..155661
                     /locus_tag="LGJ20_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBK2384272.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            155672..156508
                     /gene="eutJ"
                     /locus_tag="LGJ20_000141"
     CDS             155672..156508
                     /gene="eutJ"
                     /locus_tag="LGJ20_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBK2384273.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            156498..157685
                     /gene="eutG"
                     /locus_tag="LGJ20_000142"
     CDS             156498..157685
                     /gene="eutG"
                     /locus_tag="LGJ20_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBK2384274.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDTVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            157802..159028
                     /gene="eutH"
                     /locus_tag="LGJ20_000143"
     CDS             157802..159028
                     /gene="eutH"
                     /locus_tag="LGJ20_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBK2384275.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            159025..160428
                     /gene="eutA"
                     /locus_tag="LGJ20_000144"
     CDS             159025..160428
                     /gene="eutA"
                     /locus_tag="LGJ20_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBK2384276.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            160440..161801
                     /gene="eutB"
                     /locus_tag="LGJ20_000145"
     CDS             160440..161801
                     /gene="eutB"
                     /locus_tag="LGJ20_000145"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HBK2384277.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            161822..162709
                     /gene="eutC"
                     /locus_tag="LGJ20_000146"
     CDS             161822..162709
                     /gene="eutC"
                     /locus_tag="LGJ20_000146"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBK2384278.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRLRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            162719..163378
                     /gene="eutL"
                     /locus_tag="LGJ20_000147"
     CDS             162719..163378
                     /gene="eutL"
                     /locus_tag="LGJ20_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBK2384279.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            163391..163891
                     /gene="eutK"
                     /locus_tag="LGJ20_000148"
     CDS             163391..163891
                     /gene="eutK"
                     /locus_tag="LGJ20_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBK2384280.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            163937..164989
                     /gene="eutR"
                     /locus_tag="LGJ20_000149"
     CDS             163937..164989
                     /gene="eutR"
                     /locus_tag="LGJ20_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBK2384281.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(164995..165894)
                     /gene="hemF"
                     /locus_tag="LGJ20_000150"
     CDS             complement(164995..165894)
                     /gene="hemF"
                     /locus_tag="LGJ20_000150"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBK2384282.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(165898..166767)
                     /gene="amiA"
                     /locus_tag="LGJ20_000151"
     CDS             complement(165898..166767)
                     /gene="amiA"
                     /locus_tag="LGJ20_000151"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBK2384283.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            166981..167406
                     /locus_tag="LGJ20_000152"
     CDS             166981..167406
                     /locus_tag="LGJ20_000152"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBK2384284.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            167393..167842
                     /locus_tag="LGJ20_000153"
     CDS             167393..167842
                     /locus_tag="LGJ20_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBK2384285.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            167903..168478
                     /locus_tag="LGJ20_000154"
     CDS             167903..168478
                     /locus_tag="LGJ20_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBK2384286.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            168574..169473
                     /gene="yfeX"
                     /locus_tag="LGJ20_000155"
     CDS             168574..169473
                     /gene="yfeX"
                     /locus_tag="LGJ20_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBK2384287.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(169531..170835)
                     /gene="pbp4b"
                     /locus_tag="LGJ20_000156"
     CDS             complement(169531..170835)
                     /gene="pbp4b"
                     /locus_tag="LGJ20_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBK2384288.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(170840..172264)
                     /gene="murP"
                     /locus_tag="LGJ20_000157"
     CDS             complement(170840..172264)
                     /gene="murP"
                     /locus_tag="LGJ20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBK2384289.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(172268..173164)
                     /gene="murQ"
                     /locus_tag="LGJ20_000158"
     CDS             complement(172268..173164)
                     /gene="murQ"
                     /locus_tag="LGJ20_000158"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK2384290.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            173328..174185
                     /gene="murR"
                     /locus_tag="LGJ20_000159"
     CDS             173328..174185
                     /gene="murR"
                     /locus_tag="LGJ20_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBK2384291.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            174314..175105
                     /gene="ucpA"
                     /locus_tag="LGJ20_000160"
     CDS             174314..175105
                     /gene="ucpA"
                     /locus_tag="LGJ20_000160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBK2384292.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            175276..176292
                     /gene="cysP"
                     /locus_tag="LGJ20_000161"
     CDS             175276..176292
                     /gene="cysP"
                     /locus_tag="LGJ20_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBK2384293.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            176292..177125
                     /gene="cysT"
                     /locus_tag="LGJ20_000162"
     CDS             176292..177125
                     /gene="cysT"
                     /locus_tag="LGJ20_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBK2384294.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            177125..178000
                     /gene="cysW"
                     /locus_tag="LGJ20_000163"
     CDS             177125..178000
                     /gene="cysW"
                     /locus_tag="LGJ20_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBK2384295.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            177990..179087
                     /gene="cysA"
                     /locus_tag="LGJ20_000164"
     CDS             177990..179087
                     /gene="cysA"
                     /locus_tag="LGJ20_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBK2384296.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            179221..180132
                     /gene="cysM"
                     /locus_tag="LGJ20_000165"
     CDS             179221..180132
                     /gene="cysM"
                     /locus_tag="LGJ20_000165"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBK2384297.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(180135..180503)
                     /locus_tag="LGJ20_000166"
     CDS             complement(180135..180503)
                     /locus_tag="LGJ20_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBK2384298.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            180608..181459
                     /gene="pdxK"
                     /locus_tag="LGJ20_000167"
                     /gene_synonym="thiJ"
     CDS             180608..181459
                     /gene="pdxK"
                     /locus_tag="LGJ20_000167"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBK2384299.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(181501..182010)
                     /gene="crr"
                     /locus_tag="LGJ20_000168"
     CDS             complement(181501..182010)
                     /gene="crr"
                     /locus_tag="LGJ20_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK2384300.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(182051..183778)
                     /gene="ptsI"
                     /locus_tag="LGJ20_000169"
     CDS             complement(182051..183778)
                     /gene="ptsI"
                     /locus_tag="LGJ20_000169"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBK2384301.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(183823..184080)
                     /gene="ptsH"
                     /locus_tag="LGJ20_000170"
     CDS             complement(183823..184080)
                     /gene="ptsH"
                     /locus_tag="LGJ20_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBK2384302.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(184464..185435)
                     /gene="cysK"
                     /locus_tag="LGJ20_000171"
     CDS             complement(184464..185435)
                     /gene="cysK"
                     /locus_tag="LGJ20_000171"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK2384303.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(185620..186381)
                     /gene="cysZ"
                     /locus_tag="LGJ20_000172"
     CDS             complement(185620..186381)
                     /gene="cysZ"
                     /locus_tag="LGJ20_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBK2384304.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            186611..187597
                     /gene="zipA"
                     /locus_tag="LGJ20_000173"
     CDS             186611..187597
                     /gene="zipA"
                     /locus_tag="LGJ20_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBK2384305.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            187668..189683
                     /gene="ligA"
                     /locus_tag="LGJ20_000174"
     CDS             187668..189683
                     /gene="ligA"
                     /locus_tag="LGJ20_000174"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK2384306.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            189685..189903
                     /locus_tag="LGJ20_000175"
     CDS             189685..189903
                     /locus_tag="LGJ20_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBK2384307.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(189900..190898)
                     /locus_tag="LGJ20_000176"
     CDS             complement(189900..190898)
                     /locus_tag="LGJ20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBK2384308.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            190988..191914
                     /locus_tag="LGJ20_000177"
     CDS             190988..191914
                     /locus_tag="LGJ20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2384309.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(191953..192717)
                     /locus_tag="LGJ20_000178"
     CDS             complement(191953..192717)
                     /locus_tag="LGJ20_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384310.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAQFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            192966..193799
                     /gene="xapA"
                     /locus_tag="LGJ20_000179"
     CDS             192966..193799
                     /gene="xapA"
                     /locus_tag="LGJ20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HBK2384311.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            193859..>194701
                     /gene="xapB"
                     /locus_tag="LGJ20_000180"
     CDS             193859..>194701
                     /gene="xapB"
                     /locus_tag="LGJ20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HBK2384312.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIK"
BASE COUNT        46392 a        49422 c        52856 g        46031 t
ORIGIN      
        1 aaaggccatc agtcacgatg gcctttttgc gttggcgcag aaaaaaatgc ctgatgcgac
       61 gctgcgcgtc ttatactcgc acatatgcca gattcagcaa cggatgcggt ttccccaact
      121 tgcctacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcataatg atacgcaaca aatgtataac
      241 attttcttta ccaaaagaaa tagataaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc gattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tactttgccc acattttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgttatcgg tgtgtatgat gtgtttcgga tcgctggtta tcgcctgcct cccaggttat
      661 gaaactatag gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac cacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgccaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgtggcg ccagctcaag ttgcggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttta ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccggattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg gtcggagatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgcgtcatg ttggctaaat cttcatgagt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgcgatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcag gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca cacctccctc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagcc cggttagcca
     4381 tactttgcac caacaggccg tggatccgcg cctgcggcca tgccattttt acgcgatcga
     4441 aaatagcgtt tgccgcttgc tggacttcat tggctgtacg caggtacagc attacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggtg gggagcgtgt
     4621 tcatgccata cgcttgcagg ataggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgccgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 acgcggtgat ggtgccttcc ggcgtgcgat aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta attcttgcgc gctttctgtt gcgggagccg
     4981 ctgcgctggg tgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgagc gggttagaga
     5101 ttgccacatg tccaggcagt gcatcgcgca gtttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgtgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ctcgtgggtg tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccacgccgg atccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggccgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 caagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggcagccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc tagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattagtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cctcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgatgcg ccaattaccg
     6061 caatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgcc
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggccttcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gttgatagtc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga cgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcgccccggt
     6901 aagattaaag agattcgttc agccagctgt cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt atattgatcc agaagttttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcatgtca ggcactgttc cagagccgtc cggaagcgat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagcg gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttctt gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgtgtactg gcgctggaaa acgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggcgttgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaaccag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcagca gtgacggagg agtggcgatt
     9061 ccggggcgaa cggaaaatgc cagcccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat agggttgctg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttat ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tgcgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaggaatcaa gggttgttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaataa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcgcgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat caggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct tttttaatta atagaaaatg cgtaccgcgc aatagatcag
    10081 cagcagcgcg agcacgatat ttaactggcg accatactgg cgaaacaatc gctgaaacag
    10141 atgccccgcc agcgcccagc acacattacc aaacgtccca atcatcgcca gcaaaacgct
    10201 gacgccaact acccagctta gcgcctgtgt ttgcggcaga acaaacgtcg acagtgccgt
    10261 aacaccgtac aaaatgattt tgacgttcac aaactgcaaa gcaaagctgg cccaaaagct
    10321 gattggtttt gcctgaagtc cgtcttcctt tgttgggctg gtggcgattt tccacgccag
    10381 ccagacaata tatgccgccc ccgcccaact caaaaggtgt accgctgccg ggtcaatcac
    10441 tgccagtgaa aatgaaatgc ccgcacacag taacatcaca atcaaaaatc ccagactcat
    10501 ccctgccagc acgcgggtac tttgacgaaa tccatgcgac gtagcagagc taagggcgag
    10561 aatattgttc ggtcctggcg tcatggcggt aatcagggtg taagtccaaa aagcacttaa
    10621 aagggtcggt gtcacttgtt atctcctctg ttttggtaga cagtacccga cacggaacat
    10681 ttaaaaaaat gaatgttttt acattctaaa ttcgatattt tcgataggtt tggggttatg
    10741 gatctgcgcc gttttattac acttaaaacc gtggtggaag agggttcctt tttgcgagct
    10801 tcgcaaaaat tgtgctgtac acaatcgacg gtgacttttc atattcagca gcttgagcag
    10861 gagttctcgg tacaattgtt tgagaaaatt ggtcgacgaa tgtgccttac ccgcgaagga
    10921 aaaaagttgc tgccgcacat ctatgaactt acccgagtga tggatacgct tcgtgaggct
    10981 gcgaaaaaag agtcggatcc ggacggggaa ctacgcgttg tttcgggcga aacgttgctc
    11041 tcttaccgaa tgccgcaggt gttgcagcgt tttcggcaac gcgcaccaaa agtgcgttta
    11101 tcgttgcagt cgctgaactg ttacgtgatc cgtgatgcgc tactgaatga tgaggccgat
    11161 gtcggcgtct tttatcgcgt agggaatgat gatgccctga accgacgaga gttgggtgaa
    11221 caatcgctgg tgctggtggc ttcaccgcaa attgcagatg tcgattttac tgagccggga
    11281 agacataacg cctgtagctt tattatcaac gaaccgcaat gtgtcttccg gcagatattt
    11341 gagagcacgc tgcgtcagcg gcggatcacg gtggaaaaca ccattgagct gttaagcatt
    11401 gaaagtatca aacgttgtgt cgcggcgaat atcggtgtca gttacttgcc ccgctttgcg
    11461 gtggaaaaag agcttgagag cggagaatta attgaattgc cttttggcga acagtcgcag
    11521 accattacgg ccatgtgcgc acaccatgct ggaaaagcgg ttagcccggc gatgcacact
    11581 tttattcagt gtgttgaaga gagttttgtg gctggataaa aataatgtcg ggattaaacc
    11641 cggcatttaa cttgtcgaca aagtgcgctt tattcatgcc ggatgcggcg tgaacgcctt
    11701 atccggccta caagatcgtg aaaattcaat atattgcatg agccacgtag gcctgataag
    11761 cgtagcgcat caggcagttt tgcgtttgcc agcactttta ataccaggct taaacccggc
    11821 attgagatta ctcttctgtc gtttgcggtg gcacgcccgt tccgcttggt ttacggcgct
    11881 taccaatatt tttggtgtcg cgatggcgtt ttttcacacg cggtttctct ttctctttag
    11941 cttttttctt ctcggcgcgt tttgccagta ctttcttcga tggcttgccg gtctgcttct
    12001 cgcttggcgc acgcgttttc gggcgtaact catcaataac gcgagcttta atcggctctt
    12061 caatgtaacg gccaaccttg cccagcagca aatggtcatg ggcttccacc agcgaaattg
    12121 cggtgccttt acgaccggcg cgcgcggtac gtccgatacg gtgcaaataa gtatcgccac
    12181 tgcgcggcat atcgaagtta aagacgtggc tgacgtcagg aatgtcgata ccgcgcgcgg
    12241 caacatcggt tgcaaccagt acgtttacgc gaccttcggt caaacgcttg atcgcttcgt
    12301 tacgcttgcc ttgtaccatc tcaccttcga gatagcagtt gttgatgccc gcttcgcgca
    12361 gccagtttgc cagctcatgc acacgttcac gcttacgcac aaacacaatt gagcgggtcg
    12421 cttccggctg ttttaacaga tgcaccagca atgcggtttt atgttccagg tcatcggcgc
    12481 ggtaatacca ctgatgaatt tttttgcgct cacgggtgga cggattggca gaaacctcca
    12541 ccggatcttc cagcagacgc tcggcaaagt cctgaatcgc atcgccttcc agcgttgccg
    12601 aaaagagcag ggtctgttta cgccagcgcg tttcgccagc aatatgttcg atatcctgag
    12661 cgaagcccat atccagcata cggtctgctt cgtcgaggat cagcgtttca accgcgcggc
    12721 aatcgaagtt ctcttctttt atgtattgca gcagacgtcc ggtcgtggcg accacgatgt
    12781 cctgattttc gctgaacact tccgcgtggt tcatataggc tacgccgccg gtgatggtgg
    12841 cgatatccag atgcgtatgt ttcgccagtt cgcgggcatg atcggccacc tgcatcgcca
    12901 gctcgcgagt tggggtgagg atcaaaatac gcggcggacc ggatttctta cgcgggaaat
    12961 cgagcaggtg ctgcaacgct ggcagcagat acgccgccgt tttaccggtg cctgtcggcg
    13021 cagaaccgag tacatcacgg ccatcgagcg caggcggaat ggcggcagcc tgaatggcgg
    13081 tcgggcgagt gaaaccttta tcctggaggg cttccagcag gctttcgtcg agttcaagtt
    13141 cggaaaaagt cgttacagtc atgttctacc tctgtgtggg gcgctgatta tagacgttac
    13201 ggctgcaatc ttcatctgtt tgtatggata tcgcttctcg ggtattgttc tcacccggtg
    13261 ctatcgccgt ttttcctgca aggttttatt ttcatgtcac agtctacatc cgtgcttcgt
    13321 cgtaatggat ttacttttaa acagtttttt gttgctcacg atcgctgtgc gatgaaagtg
    13381 ggaacggatg gcattttgct gggcgcatgg gcaccggtgg ctggggtgaa acgttgcctt
    13441 gatatcggcg cgggtagcgg gttgctggca ctgatgctgg cgcagcgaac cagtgacagc
    13501 gtgataattg atgccgttga gctggaaagt gaagctgccg cgcaggcgca ggaaaatatc
    13561 aaccagtccc cgtgggcaga gcggattaac gtccatacgg cggatattca gcagtggatc
    13621 acacagcaga cagtacgctt cgatttaatc atcagtaacc caccttacta tcagcaggga
    13681 gtagagtgcg cgacacctca acgggaacag gctcgctata ccactacgct tgatcaccca
    13741 tcgctgctga cctgtgcggc ggagtgcatt accgaagagg gatttttctg cgtggtgctg
    13801 ccagagcaga ttggtaatgg ttttactgaa ctggcattaa gcatgggctg gcatttacgt
    13861 ttgcggacgg atgtggcgga aaacgaagcg cgactgccgc atcgggtgct gctggcattc
    13921 tccccgcagg cgggagaatg ctttagcgat cgcttagtca ttcgtgggcc agaccagaac
    13981 tattccgaag cgtatacggc gctgacccag gctttttatc tgtttatgta atgattgccg
    14041 ggggaaagga tcgatggacc ggaatgcgtg agcagttccg ggtaatccag cgtaaaatgc
    14101 agcccccggc tttctttgcg catcatcgcg cagcgaacaa tcaactcggc aacctgtacc
    14161 agattacgca gctccagcaa attatttgag acgcggaaat gggcgtaata ttcgtctatt
    14221 tcttgttgga gcatggttat ccgccgcagg gcgcgttcca ggcgcttcgt tgtgcgcaca
    14281 atgccaacgt aatcccacat aaacagccgt agctcgtgcc agttatgctg aattactacc
    14341 cgttcgtcag ggttctcaac gcggctttca tcccacggcg gtaacgtact gacgccgtgg
    14401 gcatcaggca tacgtctggt gatatcttct gccgccgacc agccatagac cagacactcc
    14461 agcaatgaat tcgaggccat gcggttagcg ccgtgtaagc cggtataact cacctcgcca
    14521 atggcataca agccctcgac gtccgtacgc ccatgatcat caaccattac accaccgcag
    14581 gtataatgtg cagcaggcac aatcggtacc ggttcttgtg tgagatcaat ccccagcccg
    14641 agcaattttt cataaatcat cgggaaatgc tggcgaataa aatcggcggg cttatggctg
    14701 atgtcgagga acatacaatc tgcgccgagg cgtttcattt catggtcaat ggcgcgggcg
    14761 acaatatcgc gcggggccag ttcgccgcgc tcatcaaaat cgggcataaa acgcgtgcca
    14821 tccgggcgct tgagataagc gccttcgccg cgcagtgctt ccgttaacag gaaattgcgt
    14881 gcctgtgggt gatacagtgc ggtagggtgg aactgattca attcgagatt ggcaacccga
    14941 cagcctgcgc gccacgccat agcaatgcca tcgccagaag aaatatccgg attggtggtg
    15001 tactgataaa ccttcgacgc accgccggtt gccagtacca ctgcttttgc gtggcaggtt
    15061 tccaccgttt ctttatttcg gttccatacc cacgcgccaa caacccgtcg cgtgcccggg
    15121 aggccaattt tgtcagaaac aatcagatca actgcgttgc tgcgctccag cacacgaata
    15181 ttcggatggt tctgcgcctt gctcaccagc gtggattgta cttctctacc ggtggcgtcg
    15241 gcggcatgaa gaatacgacg gtgactatgt ccaccttcac gggtcagatg gtagctttct
    15301 tcgccattcg gttgaacgtg ggtatcaaac aaaacccctt ggtcaattaa ccattgcaca
    15361 caggatcgtg cattgctggc gacaaattca actgcatggc gatcgcaaat accagccccg
    15421 gcaatcaatg tgtcttccac atgcgagtca atgctgtcag tttcatcaaa cacggcggca
    15481 ataccgccct gggcataaaa tgttgaacct tccgttaccg ggcctttact tagaacgatg
    15541 acctgatgct ggtcagccag gcgtagcgcc agtgaaagtc cggctgcgcc gctaccgata
    15601 atcaacacgt cacatgaatg ttctgggaga gtattcattt tctttgttta atttactaaa
    15661 caccgtttgg tcagcatagc atcatgttgt gcgaataaac acctgctatt ttaatatttg
    15721 ttacagtggc taaacacgct gacgcagggc ggcgagaaaa agagaagtta ctggctggtg
    15781 gaggattagg tggtgaaata aaaaggccgt tgggttactc ttcaggcagt taaatgggca
    15841 tttctacaca gataatgcga tgttcagatt ctgtagactt ataatgatag ataatgatcc
    15901 gtctacagca tgacaaacaa aaacagatgc gttacggaac tttacaaaaa cgagacactc
    15961 taaccctttg cttgctcaaa ttgcagctaa tggagtggcg tttcgatagc gcgtggaaat
    16021 ttggtttggg gagactttac ctcggatgag cgagcagtta acggaccagg tcctggttga
    16081 acgggtccag aagggagatc agaaagcctt taacctactg gtagtgcgct atcagcataa
    16141 agtggcgagt ctggtttccc gctatgtgcc gtcgggtgat gttcccgatg tggtacaaga
    16201 agcttttatt aaagcctatc gtgcgctgga ttcgttccgg ggagatagcg ctttttatac
    16261 atggctgtat cggattgctg taaatacagc gaaaaattac ctggttgctc aggggcgtcg
    16321 tccaccttcc agtgatgtgg atgccattga agctgaaaac ttcgaaagtg gcggcgcgtt
    16381 gaaagaaatt tcgaaccctg agaacttaat gttgtcagaa gaactgagac agatagtttt
    16441 ccgaactatt gagtccctcc cggaagattt gcgcatggca ataaccttgc gggagctgga
    16501 tggcctgagc tatgaagaga tagccgctat catggattgt ccggtaggta cggtgcgttc
    16561 acgtatcttc cgagcgaggg aagctattga taacaaagtt caaccgctta tcaggcgttg
    16621 acgatagcgg gatactggat aagggtatta ggcatgcaga aagaacaact ttccgcttta
    16681 atggatggcg aaacgctgga tagtgagctg cttaacgaac tggctcataa cccagaaatg
    16741 cagaaaacct gggaaagcta tcacttaatc cgtgactcaa tgcggggtga tactcccgag
    16801 gtgctccatt tcgatatctc ttcacgcgtg atggccgcca ttgaagaaga gccagtacgt
    16861 caaccggcga cattgatccc ggaagcccag cctgcgccgc atcaatggca gaaaatgcca
    16921 ttctggcaga aagtacgtcc gtgggcggca cagcttaccc aaatgggcgt agcagcatgc
    16981 gtatcgcttg cagttatcgt tggcgtccag cactataatg gacaatctga aacgtcccag
    17041 cagcccgaaa cgccggtatt taatacactg ccgatgatgg gtaaagccag cccggtaagc
    17101 ctgggagtac cttctgaagc gaccgcaaac aatggtcaac agcagcaggt acaggagcag
    17161 cgtcgtcgca ttaatgcaat gttgcaggat tacgaactgc aacgccgact ccactctgaa
    17221 cagcttcagt ttgagcaggc gcaaacccag caagccgctg tacaggtgcc aggaattcaa
    17281 actttaggaa cgcaatcgca gtaatgaagc aactttggtt tgccatgtca ttagtgacag
    17341 gtagcctgtt attctctgct aacgcctcgg ccactcccgc gtccggggcg ttattacagc
    17401 agatgaacct ggccagtcag tcactgaatt acgagctgtc attcatcagc atcaataaac
    17461 agggtgttga gtctctgcgt tatcgacatg cacgcctcga taaccgccct cttgcacaat
    17521 tgttgcaaat ggatggcccg cgccgggaag tggtacagcg cggcaatgaa atcagctatt
    17581 ttgaaccggg acttgaaccg ttcacgctta atggcgatta cattgttgat tctctgccat
    17641 cgcttatcta taccgatttc aaacgccttt ctccttacta cgactttatc tccgtcgggc
    17701 gcacgcgtat tgctgatcgt ctttgcgaag tcattcgcgt ggttgcccga gatggtacac
    17761 gctacagcta catcgtgtgg atggacaccg aatcgaaatt accgatgcgg gttgatctcc
    17821 ttgatcgcga tggtgaaacg ctggaacaat ttcgcgtgat tgcttttaac gtcaatcagg
    17881 atatcagcag cagtatgcag acgctggcga aggcaaattt gccgccgttg ctttctgttc
    17941 ctgtaggtga aaaagctaaa ttcagctgga cgccaacctg gttgccacag ggttttagcg
    18001 aagtttccag tagtcgacgt ccgctaccga cgatggacaa catgcctatc gaatcacgtc
    18061 tctattccga cggattattc agcttctcgg taaacgttaa ccgcgctacg ccatcgagca
    18121 ccgatcagat gttgcgcacc ggacgcagaa ccgtcagtac aagcgtacgt gataacgccg
    18181 aaatcaccat tgtcggtgaa ctgccgccgc aaacggcgaa acgcattgcc gagaatatta
    18241 agttcggggc agcgcaatga tcaaagagtg ggctaccgtc gtctcctggc aaaacgggca
    18301 ggcgctggtc agttgtgatg ttaaagcttc atgcagcagc tgcgcttcac gcgccggttg
    18361 cggtagccgc gtgttaaata aacttggccc gcaaaccacg cataccattg tcgtaccctg
    18421 tgatgagccg ttagtgccgg ggcaaaaagt ggaattgggg atcgccgaag gcagcctgct
    18481 tagctccgca ttactggttt atatgtcgcc gctggtggga ttattcctca tcgcttcgct
    18541 atttcagcta ctctttgctt ctgacgtcgc agcattatgt ggtgcggtcc tcggcggtat
    18601 tggcgggttc ctgattgcgc gcggctactc gcgtaagttt gctgcccggg cagaatggca
    18661 accgatcatc ttaagcgtgg cattgccgcc tggccttgtg cgatttgaaa catcttcgga
    18721 agacgcgagc cagtgatttc actggctttg ttgcattttg cataacaaat gcgaggacgt
    18781 ttccagatac cacaatccat acggtatgaa catttcctcg cctcaatgtt gtagtgtaga
    18841 atgcggcgtt tctattaata cagacgttaa gctcagaaca gcgacttcta aaagcctggt
    18901 taaccgggca ttaaggcaca ataatcatac tttatgaaga atatacgtaa cttttcgatc
    18961 atagctcaca ttgaccacgg taaatcgacg ctgtctgacc gtattatcca gatctgcggt
    19021 ggcctgtctg accgtgaaat ggaggcgcag gttctcgatt ccatggatct tgagcgtgag
    19081 cgtggcataa ccatcaaagc gcaaagcgtg acgctggact acaaagcgtc tgacggcgaa
    19141 acctatcagc tgaactttat cgacaccccg ggccacgttg acttctccta tgaagtttcc
    19201 cgttcgctgg ctgcctgtga aggtgcattg ctggtggtcg acgccgggca gggcgtagaa
    19261 gcgcaaaccc tggcaaactg ctacaccgcc atggaaatgg atctcgaagt ggtgccggta
    19321 ctgaacaaga ttgacctgcc ggcagccgat cctgaacgcg tggcggaaga aattgaagat
    19381 atcgtcggca tcgacgccac cgacgcggtg cgctgttcag cgaaaaccgg cgttggcgtg
    19441 caggacgttc tcgaacgtct ggtgcgcgac attccgccgc cggaaggcga tccggaaggc
    19501 ccgttgcagg cactaattat cgactcatgg ttcgacaact acctgggcgt tgtttcactt
    19561 atccgtatta aaaacggcac cctgcgtaag ggcgacaaag tgaaagtcat gagtaccggg
    19621 cagacctata acgccgaccg tctgggcatc ttcacgccga aacaggttga ccgcactgaa
    19681 ctgaaatgtg gcgaagtagg ctggctggta tgtgcgatta aagatatcca cggcgctcca
    19741 gtcggcgata ccttaacgct ggcgcgtaat ccggcagaaa aggcgctgcc tggctttaag
    19801 aaagtcaaac cgcaggtata cgccggtctg ttcccggtaa gttccgacga ctatgaagcc
    19861 ttccgtgacg cgctgggtaa actcagcctg aacgatgctt cactgttcta tgagccggaa
    19921 agctccagcg cgctgggctt tggtttccgc tgcggcttcc tcggcctgct gcacatggag
    19981 atcatccagg aacgtctgga acgtgaatac gatctggatc tgatcaccac tgcgccgacc
    20041 gtagtgtatg aagttgaaac cacgtcaaga gaagttattt acgtcgacag tccatccaag
    20101 ctgccagcgg taaataacat ctacgaactg cgcgagccga ttgccgaatg tcacatgctg
    20161 ctgccgcagg catatctcgg caacgttatt acgctgtgcg tagaaaaacg cggcgtgcag
    20221 accaatatgg tttaccacgg taatcaggtg gcgctgacgt acgagatccc gatggcggaa
    20281 gtggtgctcg acttcttcga tcgcctgaaa tctacctcgc gtggttatgc gtctctggat
    20341 tacaacttca aacgcttcca ggcgtccgac atggtacgtg tagacgtatt aatcaacggt
    20401 gaacgtgttg atgcgttggc gctgatcacc caccgtgata attcgcaaaa ccgcggtcgc
    20461 gagttggtgg agaagatgaa agatctgatc ccacgccagc agtttgatat cgccattcag
    20521 gcagcgattg gtacgcacat tattgcgcga tccaccgtga aacagctgcg taaaaacgta
    20581 ctggctaaat gttatggcgg cgatatcagc cgtaagaaaa agctgctgca gaagcagaaa
    20641 gaaggtaaga aacgcatgaa gcagatcggt aacgtcgagc tgccgcagga agcgttcctc
    20701 gccattctgc acgtcggcaa agacaacaaa taacccttag gagttggcat ggcgaatatg
    20761 tttgccctga ttctggtgat tgccacactg gtgacgggca ttttatggtg cgtggataaa
    20821 ttctttttcg cacctaaacg gcgggaacgt caggcagcgg cgcaggcggc tgccggtgac
    20881 tcactggata aagcaaccct gaaaaaggtt gcgccgaagc ctggctggct ggaaaccggt
    20941 gcttctgttt ttccggtgct ggctatcgta ttgattgtgc gttcgtttat ttatgaaccg
    21001 ttccagatcc cgtcaggttc gatgatgccg actctgttaa tcggtgattt tattctggta
    21061 gaaaagtttg cttatggcat taaagatcct atctaccaga aaacgctgat cgaaaccggt
    21121 catccgaaac gcggagatat cgtggtcttt aaatatccgg aagatccaaa gcttgattac
    21181 atcaagcgcg cggtgggttt accgggcgat aaagtcactt acgatccggt ctcgaaagag
    21241 ctgacgattc aaccgggatg cagttccggc caggcgtgtg aaaacgcgct gccggtcacc
    21301 tactcaaacg tggaaccgag cgatttcgtt cagaccttct cacgccgtaa tggtggggaa
    21361 gcgaccagcg gattctttga agtgccgaaa aacgaaacca aagaaaatgg aattcgtctt
    21421 tccgagcgta aagagacact gggtgatgtg acgcaccgaa ttctgacagt gccgattgcg
    21481 caggatcagg tggggatgta ttaccagcag ccagggcaac aactggcaac ctggattgtt
    21541 cctccgggac aatacttcat gatgggcgac aaccgcgaca acagcgcgga cagccgttat
    21601 tgggggtttg tgcctgaagc gaatctggtc ggtcgggcaa cggctatctg gatgagcttc
    21661 gataagcaag aaggcgaatg gccgactggt gtgcgcttaa gtcgcattgg cggcatccat
    21721 taatagctca tcttcgttca cgttgtcgcc gttatggcga caacgtgaat tatttatgag
    21781 ataaatctcc cgtggctaac gacatccccc gtcgttgtgt atagaatatt cccccgaagt
    21841 ttaaggttgg cacctccagg ttgccacggc acacgaaaca gcgttggtcc ccatataccg
    21901 gtaaactgaa actgcagcga agcagttagc agagccatgt atatcaggtc tgtttcgtgt
    21961 gctgaattgt tgacgcattt atttattggt atcgcatgaa ccccatcgta attaatcggc
    22021 ttcaacggaa gctgggctac acttttaatc atcaggaact gttgcagcag gcattaactc
    22081 atcgtagtgc cagcagtaaa cataacgagc gtttagaatt tttaggcgac tctattctga
    22141 gctacgttat cgccaatgcg ctttatcacc gtttccctcg tgtggatgaa ggcgatatga
    22201 gccggatgcg cgccacgctg gtccgtggca atacgctggc ggaactggcg cgcgaatttg
    22261 agttaggcga gtgcttacgt ttagggccag gtgaacttaa aagcggtgga tttcgtcgtg
    22321 agtcaattct cgccgacacc gtcgaagcat taattggtgg cgtattcctc gacagtgata
    22381 ttcaaaccgt cgagaaatta atcctcaact ggtatcaaac tcgtttggac gaaattagcc
    22441 caggcgataa acaaaaagat ccgaaaacgc gcttgcaaga atatttgcag ggtcgccatc
    22501 tgccgctgcc gacttatctg gtagtccagg tacgtggcga agcgcacgat caggaattta
    22561 ctatccactg ccaggtcagc ggcctgagtg aaccggtggt tggcacaggt tcaagccgtc
    22621 gtaaggctga gcaggctgcc gccgaacagg cgttgaaaaa actggagctg gaatgagcat
    22681 cgataaaagt tactgcggat ttattgccat cgtcggacgt ccgaacgttg gcaaatccac
    22741 attgttgaac aaactgctgg ggcagaagat ctccatcacc tcccgcaaag cgcaaacaac
    22801 tcgtcaccgc attgtgggga tccatactga aggcgcgtat caggcgatct acgttgatac
    22861 ccctggcctg catatggaag aaaaacgcgc tattaaccgc ctgatgaaca aagcggcgag
    22921 cagctctatt ggcgacgttg agctggtgat ttttgtcgtt gaaggcaccc gctggacgcc
    22981 ggacgacgaa atggtgctca acaaactgcg cgatggcaaa gcgccggtga tcctcgcggt
    23041 gaacaaagtg gataacgtgc aggaaaaagc cgatctgctg ccgcacttac agttcctggc
    23101 aagtcagatg aacttcctcg atatcgtgcc gatctctgct gaaaccgggc tgaatgtcga
    23161 cactatcgca gcaatcgtgc gtaagcacct ccctgaagcg acccatcact tcccggaaga
    23221 ttacatcacc gatcgctcac agcgttttat ggcgtctgaa atcatccgcg aaaaactgat
    23281 gcgtttcctc ggcgctgaac tgccgtactc cgtgaccgtg gagatcgaac gtttcgtctc
    23341 taacgaacgc ggtggttatg acatcaatgg tttgattctc gttgagcgtg aagggcagaa
    23401 gaagatggtt attggcaaca aaggggccaa gatcaaaacc atcgggattg aagcgcgtaa
    23461 agacatgcag gaaatgttcg aagcgcctgt ccaccttgaa ctgtgggtaa aagtgaaatc
    23521 cggttgggcc gacgacgaac gcgcactgcg cagtctcggt tacgttgacg atctttaaga
    23581 gtcactccga tggaaggctg gcagcgcgca tttgtcctgc atagtcgccc gtggagcgaa
    23641 accagcctga tgctggacgt cttcacggag gaatcgggtc gcgtgcgtct ggttgccaaa
    23701 ggcgcacgct ctaaacgctc tactctgaaa ggtgcattac agcctttcac ccctctcttg
    23761 ctacgtttcg gcgggcgtgg cgaagtcaaa acgctgcgca gtgctgaagc tgtctcgctg
    23821 gcgctgccat taagcggtat cacgctttac agcggtctgt acatcaacga acttctctcc
    23881 cgcgtactgg aatacgagac gcgcttctct gaactctttt tcgattactt gcactgcatt
    23941 cagtctcttg caggtgccac tggtacgcca gaacccgcgc tgcgccgctt tgaactggca
    24001 ctgctcgggc atctgggcta tggcgtcaat tttacccatt gtgcgggtag cggcgagccg
    24061 gtagatgaca ccatgacgta tcgttatcgc gaagaaaaag ggtttatcgc aagcgtcgtt
    24121 atcgacaata aaacgttcac cggaaggcag ttaaaagcgt taaacgcgcg ggaatttcct
    24181 gacgcagaca cactacgcgc cgcgaaacgc tttacccgca tggcgcttaa gccgtatctt
    24241 ggcggtaaac cattaaagag cagggaactg ttccggcagt ttatgcctaa gcgaacggtg
    24301 aaaacacatt atgaatgatg aggattgtca tggctgaatt actgttaggc gtcaacattg
    24361 accatattgc aacgctgcgt aacgcacgcg gtactgctta cccggatccg gtgcaggcgg
    24421 cgtttattgc tgagcaggcg ggagcggacg gcattaccgt gcatttacgt gaagatcgcc
    24481 gccacattac tgaccgtgat gtgcgcatcc tgcgtcagac actggatacc cgcatgaatc
    24541 tggagatggc ggtgaccgaa gagatgctgg cgatcgccgt tgagacgaag ccacattttt
    24601 gctgtctggt accggaaaag cgtcaggaag taacaaccga aggcggcctg gacgtcgcag
    24661 ggcagcgtga caaaatgcgc gatgcctgca aacgtctggc agatgccggg atccaggttt
    24721 ctctctttat cgacgccgat gaagagcaga tcaaagctgc ggcagaggtt ggcgcaccgt
    24781 ttatcgagat ccacaccggt tgctatgctg atgccaaaac tgacgccgaa caggcgcaag
    24841 agctggcgcg tatcgccaaa gccgcgacct ttgccgctag tctcggtctg aaagttaacg
    24901 ctggtcacgg tctgacctat cacaacgtga aagctattgc cgccatccct gagatgcatg
    24961 aactgaatat cggtcatgcc attattggtc gtgcagtgat gaccggactg aaagatgcgg
    25021 tggcagaaat gaagcgtctg atgctggaag cgcgtggcta atggcaatat taggtttagg
    25081 cacggatatc gtagagatcg ctcgcataga agcagtgatc gcccgatccg gtgagcgcct
    25141 ggcacgccgc gtattaagcg ataacgaatg ggcaatctgg aaaacgcacc accagccggt
    25201 gcgttttctg gcgaagcgtt ttgctgtgaa agaagccgca gcaaaagcgt ttggcaccgg
    25261 gatccgcaat ggtctggcgt ttaatcaatt tgaagtattc aatgatgagc tcggcaaacc
    25321 acggctacgg ctatggggtg aggcattaaa actggcggaa aagctcggcg ttgtaaatat
    25381 gcatgtaacg ctggcagatg aacgccacta tgcttgtgcc acggtaatta ttgaaagtta
    25441 aaataatacc gttctcttta aaaagtaaaa cccccgaact ggtaaggaac gggggtttta
    25501 ctttttaact ttaattttgt gcagcatgca ccctcaacaa cagcgattat ggatcagtgt
    25561 acttatcttt tacaagcaac ttgcaatctt tagcataaaa actcgagcct ttacgaagaa
    25621 agcaatattg atggaaagat taacgtgacc gccaattcgt aagtacatta aaattggctt
    25681 cgttattgaa gattttgctg tgctttacac catgccacag aattccccca ttgaaacgag
    25741 tggtgtcgtc aaagctctgg tgtggagtgc agcatgcacc ctcaataact cgcacgttca
    25801 gttttgggga gatgtaaggg ctaatctgaa cagctgcatt ccttgtttaa ggaaaaacga
    25861 atgactgatt gccgatacct gattaaacgg gtcatcaaaa tcatcattgc tgttttacag
    25921 ctgatccttc tgttcttata acacaaggaa acgtacttaa ggtgcgtccg gtgagccaat
    25981 cggacgcgcc tttaataact ataaataagt gtcttggcag atactatata aattaactta
    26041 gtgaatgatt atgctaatgt catcaattaa ataaatataa tggcgttaag gcttcccagt
    26101 aatataatta atactctact tccagagtag aatattaaat tttatccgcg tggtgcatca
    26161 gcacaaattt atcccacaac tgttcttctg tctcgacatg cgccggatct ttcacaatag
    26221 tattggggat cgggcacacc ttctggcagg ttggtgtctc gtagtgccct acgcattcgg
    26281 tacacttatc gctgttaatc tcgtagatat gatctcccat tgaaatcgcc tcattcgggc
    26341 attcgggttc acacatatca caattgatgc attttttagt gattaacaac gccataagga
    26401 aactctcaaa acaacacaaa ccgggcgggc attatacgcg cattctttac tcagaccagt
    26461 tttttcacca gcgcttcact atgacgaata cgttctggtg ccagttccag atcctgctga
    26521 atcagcgcaa tgaacagcaa atccgttaac attccctgag cgtgacaagc agagattgaa
    26581 gcactgtttg tcgcttgttc ttcggcaatg gtatacaggc aatgagaagc acgctgttgc
    26641 agggcattcg gagtaaagcc ggtaatcgcc agcacttttc cgcccactcg cagcatctca
    26701 tctgccgcca ggtttaactc gcgtcgtaca ccggtgtagg aaatggctaa taacagatcg
    26761 tcaggggacg acgcctgtac tgttgcgagt agcgcatgca tatcgcgcac tgcggcagca
    26821 ttgaagccaa tcttcatcag cttccaggca aagttttgcg ccaccagacc cgaagcgcca
    26881 ataccggtca gaattatccg ccgcgcagag cgcaacattg ttacgcattc atgcagtttc
    26941 tcttcactat taacgtttag cgttgcgtac atagcggcag tattttcttt aatcagtttt
    27001 tcgccgacca gccgtaacgg atcatcaccg cggatttggt tatgaatggg cacggagggc
    27061 gattccggct ggcttgccag cgcttcactc aacgccaact taagcgccgg aaaaccttta
    27121 tagccgagtt tttgcgcgaa cttcacgacg ctggactgac tgactccggc ttcgttggcc
    27181 agttgctgag agcttaaatg gcgcgccgta tcaggttgta gcagcagata atccgccagt
    27241 tttttatcac tctgggcaag cccctggtaa cgctgacgga tacgaagtaa gccgttcata
    27301 tctttcctcg cgatgaaata gtggcgacaa caggcaatat gagagacacg cgagtgaatt
    27361 ttatattcca ttgccgctaa ttgccatgaa ttaaaaattc cagttagcca tgatgcggtc
    27421 gattgttgcc ggaaaagtga tgatctatct gttaggctat gtagtagcga aaattaatca
    27481 ggatgtttca gtccagagga gtatggtttg gcaactcacg agcgtcgtgt ggtgtttttt
    27541 gacttagatg gaacattgca tcagcaggat atgttcggca gttttctgcg ctatttactg
    27601 cgtcgccaac cgctgaatgc gttacttgtc ctgccgttgt taccgattat agccattgcg
    27661 ttattgataa aaggtcgtgc ggcacgctgg ccgatgagtc tgcttctgtg ggggtgcact
    27721 tttggtcaca gcgaagcacg tttacagacg ttgcaggccg atttcgtgcg ctggtttcgc
    27781 gacaatgtta ccgcctttcc gctggttcag gagcgattaa ccacctacct gttaagttcc
    27841 gatgctgata tctggttgat taccggctct ccgcagccgc tggttgaagc ggtttatttc
    27901 gatacgccct ggctgccgcg ggttaatctt atcgccagcc aaattcagcg tggctatggt
    27961 ggttgggtat tgacgatgcg ttgtctggga catgaaaagg tcgcacaact ggagcgcaaa
    28021 atcggcactc cgctgcggct gtacagtggc tatagcgaca gtaatcagga caatccgctg
    28081 ctttatttct gtcagcatcg ttggcgagta accccgcgcg gtgaactcca gcaactggaa
    28141 tagagtaaag catagcgtcc gtgtataatg cgccgcgctt ttataaccgg agttttcttt
    28201 ttgtctgaag tcgaatttag ccacgaatac tggatgcgtc acgcgctgac gctggcgaaa
    28261 cgtgcctggg atgagcggga agtgccggtc ggcgcggtat tagtgcataa caatcgggta
    28321 atcggcgaag gctggaaccg cccgattggt cgccatgatc ccaccgcaca tgcagaaatc
    28381 atggccctgc ggcagggtgg tctggtgatg caaaattatc gtctgatcga cgccacgttg
    28441 tatgtcacgc ttgaaccatg tgtaatgtgt gccggagcga tgatccacag tcgcattggt
    28501 cgcgtggtct ttggtgcgcg tgacgcgaaa actggcgctg cgggatcttt aatggatgtg
    28561 ctgcatcatc cgggtatgaa tcaccgagtg gaaattacgg aaggaatact ggcggatgag
    28621 tgcgcggcgt tgctcagtga cttctttcgc atgcgccgcc aggaaattaa agcgcagaaa
    28681 aaagcgcaat cctcgacgga ttaattttgt ttctcttcac tccctttcct ggaaaacagc
    28741 agagagggag aatgggtcaa atagtttgat gacgactggg aaagaaacga gagaaaagga
    28801 aatttctctt tgcccaactc cgtaggcgat accgccggat aatcctgcgc cagttgcatc
    28861 gccgcttctg tagcctgctt ctctttctct tgcagataac ccaccaggct aatctgatac
    28921 ttacgaatat tttcgacata agcgtaggct tcatgtccac gagcgtagcc gtaagtcagc
    28981 ttgctgtaat agggtttctg gctaagtaaa ggcagacgct gttttacgtc agcccaactg
    29041 tcaggattcc ctttggtttt tgccgtcagg gcgcgggcat ccagcatatg cgcatagccc
    29101 atattgtacg cagcgagggc aaaccagatc cgctcgttct ccggcacact ttccggcact
    29161 ttactcatca tatcctgcaa ataacgcacg ccaccgctga tgctctgttc ggcatcggta
    29221 cgatccgtaa tgccgaggct ttgcgcggta tttttggtta acatcatcat gccgcgcaca
    29281 cccgtcggtg aagtggcctg tgcatcccag tgcgattcct gataagcaat agcggccagc
    29341 aaacgccagt caatttcttc ggcgtatttc tcaaacaggg gctttaactg cggcagtacc
    29401 gcatcgacgg cgcgtaaaaa tgtgcgcgta tcgacgtaat caaaatcatc gccatgcccc
    29461 aggtatttct cttcaatgcg tgccagcgta ccgtcttcat tcatttcgtt gaagaagtcg
    29521 agcagggcgg cggaaagggt attatcgcca tctaacgggc taaaccaagt caccggttgt
    29581 tcatcggtga tatcgagcgc tacggcgagc tccgggtgaa cgcgctgaaa caggctgatg
    29641 gcgacagaat cagcaatggt gtaatcgagt tttccttcga tgacatcttc cattaattcc
    29701 gcagagcctt ttttgtcgtc taccttccag cttaattccg ggaattttgt gtctttcagg
    29761 gtttggagat cgttaaccac cacgtgaccc ggtgcaacgg tgagttgctc cgccgtcagg
    29821 ttgcccagtg tgcgcgggcg atactgaccc actttataaa ccagttgttg tgacacggaa
    29881 taataggtag ggccaggctg ataatttttt acccgctcac tgttatagac cagtcctgcc
    29941 gccagcaggt cggcgttacc attatcgagg tcgtcaaaca gctggctgat attctgccgc
    30001 acggtcactt tcagttttac accgaggtaa tcggcaaact gtttcgccag ttcgtaatcc
    30061 aggccaaaag gtttcccgtt gatttcgtta taagtcaggg gagtatgaat ggtgctcaca
    30121 cgcaactctc cccgcgcttg aatggcggcg atacggttgt cggctttacc aaaccaggga
    30181 atggatggcc agagagcgac cgcgagcagc agtgccagaa tgccgatgaa cagataatta
    30241 atctttaatt ttttcaatta gttaattctc tgtgtcgtgc gcgtcccagc ttgaaaaaac
    30301 gtaataatag tgaaaggttt actcataaat gagcggcatt ttgcgtaaac ctgcgccaga
    30361 tggcaactta ttacagccat tggcggcacg cgttgctaat tcacgatggt gattttattt
    30421 ccacgcaaac ggtttcgtca gcgcatcaga ttctttataa tgacgcccgt ttccccccct
    30481 tgggtacacc gaaagcttag aagacgagag acttatgatg gaaattctgc gtggttcgcc
    30541 tgcactgtcg gcattccgaa tcaacaaact gctggcacgt tttcaggctg ccaggctccc
    30601 ggttcacaat atttacgccg agtatgtcca ttttgctgac ctcaatgcgc cgttaaacga
    30661 tgatgagcac gcacaacttg aacgcctgct gaaatatggc ccggcactcg ccagccacgc
    30721 cccgcaaggc aaactcctgc tggtgacccc gcgtcctggc accatctctc cctggtcttc
    30781 gaaagcgacc gatattgccc ataactgcgg gctacaacag gtaaaccgcc ttgagcgcgg
    30841 cgttgcttac tatatagaag ccggtacgct gaccaatgaa caatggcagc aggttaccgc
    30901 tgaactgcac gaccgcatga tggaaacggt cttttttgct ttagatgatg cagagcagct
    30961 gtttgcacac catcaaccga ctccggtcac cagcgttgat ttgctggggc agggccgtca
    31021 ggcgctaatc gacgctaacc tgcgtcttgg cctggcactg gcggaagatg aaattgacta
    31081 tctgcaggat gctttcacaa agcttggtcg taacccgaac gacatcgagc tgtatatgtt
    31141 cgcccaggcg aactccgagc actgccgcca caagattttt aacgccgatt gggttatcga
    31201 cggtgagcaa cagccgaaat cgctgtttaa gatgatcaaa aacaccttcg aaaccacgcc
    31261 agattacgtt ctctctgctt ataaagataa cgccgccgta atggaaggtt ctgaagtggg
    31321 ccgctacttt gctgaccacg aaacgggccg ctacgatttc catcaggaac cggcgcatat
    31381 tctgatgaaa gtcgaaactc acaaccaccc gacggcgatt tctccgtggc cgggcgcggc
    31441 gaccggttcc ggcggtgaaa tccgcgatga aggtgccacc gggcgcggcg cgaagccgaa
    31501 agcgggtttg gttggtttct ccgtttccaa cctgcgaatt cctggcttcg aacagccgtg
    31561 ggaagaagat ttcggtaagc ctgagcgtat tgtcaccgcg ctggacatca tgaccgaagg
    31621 cccgctgggc ggcgcggcgt ttaacaacga atttggtcgt ccggcactga atggctactt
    31681 ccgtacttat gaagaaaaag tgaacagcca caacggcgaa gagctgcgcg gttatcacaa
    31741 accgatcatg ctggcgggcg ggatcggcaa cattcgcgcc gatcacgtac aaaaaggcga
    31801 gatcaacgtc ggtgcgaagc tggtcgttct cggcggcccg gcaatgaaca tcggtcttgg
    31861 cggcggagcg gcgtcttcca tggcgtcagg tcagtctgat gccgacctcg actttgcttc
    31921 cgtacagcgc gacaacccgg agatggagcg tcgttgccag gaagtgatcg accgttgctg
    31981 gcagcttggt gatgctaacc caattctgtt tatccacgac gtcggtgcgg gcggtctttc
    32041 taacgcgatg ccggagctgg tgagcgacgg cggacgcggc ggtaaatttg aactgcgcga
    32101 tattctaagc gacgaaccgg gcatgagccc gctggaaatc tggtgtaacg aatcccagga
    32161 acgctacgtg ctggcggttg cagccgatca attaccgctg tttgacgaac tgtgtaagcg
    32221 tgagcgcgca ccctacgcgg tgattggtga agcgaccgaa gaactgcatc tttctctgca
    32281 cgatcgtcat tttgataatc agccgatcga tctgccgctg gacgtcctgc ttggtaaaac
    32341 gccgaagatg acccgcgatg tacaaacgct gaaagcgaag ggcgatgcgc tggtccgtga
    32401 agggatcacc attgcagacg cggtgaaacg cgtactgcat ctgccgaccg tggcggagaa
    32461 aactttcctg gtgaccatcg gtgaccgcag cgtaactggc atggtagcgc gcgatcagat
    32521 ggtgggtccg tggcaggtgc cggtcgctaa ctgcgcggtc actaccgcca gcctcgacag
    32581 ctactacggt gaagcgatgg cgattggcga gcgtgcgccg gttgcgctgc tggatttcgc
    32641 cgcctctgcc cgtctggcgg tcggtgaagc gttaaccaat attgccgcaa cacagattgg
    32701 cgatatcaaa cgcattaaac tttccgccaa ctggatggcg gcggcaggcc accctggtga
    32761 agatgcgggc ctgtatgaag ccgttaaagc cgtgggcgaa gagctttgtc cggcgctggg
    32821 cctgacgatc ccggtgggta aagactctat gtcgatgaaa acccgctggc aggaaggtaa
    32881 cgaagagcgc gaaatgacgt cgccgctgtc gctggtgatt tctgcatttg cccgcgtgga
    32941 agatgtacgt cacaccatca cgccgcagct ttctaccgaa gataacgcac tgctgctgat
    33001 tgatttgggc aaaggcaata acgcgctggg cgcaacggcg ctggcgcagg tttatcgtca
    33061 gcttggcgac aaaccggcag atgtacgcga tgtcgcgcaa ctgaaaggct tctatgacgc
    33121 gattcaggcg ctggttgcac agcgtaagct gctcgcatac cacgaccgct ctgatggcgg
    33181 cctgctggta acgctggcgg aaatggcctt tgctggtcat tgcggcatta acgcggatat
    33241 tgcctctctg ggcgacgatc gcctggcggc gttgtttaac gaagaactgg gtgcggtgat
    33301 tcaggttcgt gccgctgacc gtgaagcggt cgagtccgta ctggcacagc atgggcttgc
    33361 tgattgtgtc cattatgtag ggcaggcggt ttccggtgac cgttttgtga ttaccgccaa
    33421 cgggcagact gtattcagcg aaagccgcac cacgttgcgt gtctggtggg cagaaactac
    33481 ctggcagatg cagcgcctgc gtgacaaccc ggagtgtgct gaccaggagc accaggcgaa
    33541 atcaaacgac gccgatccgg gcctgaatgt gaaactgtcg ttcgatatca acgaagatgt
    33601 ggcagcaccg tatattgcca ctggcgcacg tccgaaagtt gctgtgctgc gtgagcaggg
    33661 cgtgaactcg catgttgaaa tggcggcagc tttccaccgt gcaggcttcg atgcaatcga
    33721 tgtgcatatg agtgacctgc tgactggacg cacgggcctg gaagatttcc acgccctggt
    33781 cgcgtgcggt ggtttctcct acggtgatgt gctgggtgcc ggtgaaggtt gggcgaagtc
    33841 aatcctgttc aatgaccgtg tacgcgatga gtttgcaacc ttcttccacc gtccgcaaac
    33901 gctggcgctg ggggtatgta acggttgcca gatgatgtct aacctgcgcg aactgatccc
    33961 aggtagtgag ttgtggccac gttttgtgcg caatacctcc gatcgctttg aagcgcgttt
    34021 cagcctggtt gaagtgaccc aaagcccgtc gctgctgttg caggggatgg tgggctcgca
    34081 aatgccgatt gctgtctctc atggtgaagg gcgcgtggaa gtgcgtgatg cggcgcatct
    34141 ggcggcactg gaaagcaaag ggctggtggc actgcgctat gtcgataact tcggcaaagt
    34201 cactgaaacc tacccggcta acccgaacgg ttcaccgaac ggtattacgt cgattacgac
    34261 tgaaagtggt cgagtcacca ttatgatgcc gcacccggaa cgtgttttcc gtactgtcag
    34321 caactcctgg catccggaaa actggggcga ggatggccca tggatgcgca ttttccgcaa
    34381 tgcgcgtaag cagttggggt aagtcatcag ttcattggtt ttgcagcccg gatgcggcat
    34441 ttacgccgca tccggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    34501 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    34561 actcattgat tttaatatta tctaataagt ttatcttaag ttgtctcttt ttagcgacac
    34621 agtggctgat ttacattcaa ataacggaga agccataaac ccctcaaagc gacgtataat
    34681 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    34741 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    34801 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    34861 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    34921 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    34981 aaacgctggc ctgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    35041 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    35101 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    35161 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgatcct
    35221 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    35281 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    35341 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    35401 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    35461 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    35521 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    35581 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    35641 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    35701 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    35761 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    35821 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    35881 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    35941 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    36001 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    36061 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    36121 gaatccggta acatttgcct tcgcagcagc ttacatggtg cacgggttta tattgatgtc
    36181 atcaatacag gcacacccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    36241 caaggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    36301 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    36361 gtttgtttcc gcattgaatt gccgtcgtcg aaaaacacga aataatgcta acagcctatc
    36421 ccatcaggct attttatttg ccagttcggc cccgggcagt gctcgaaatc ctcacgtacg
    36481 acgtgtacgc tccggtttct ccgcgctgtc catgtccaaa ctgcctgcaa taattacgcc
    36541 cactgggata ggcattaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    36601 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagagtcaca
    36661 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    36721 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    36781 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcacgtc
    36841 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    36901 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgc attgatgcgc
    36961 taagtaccga gatcccggca caggttcgcc ctctgtatca actctggcgc gacggtcagg
    37021 cgctgcaact gcaactggcg gaagagcgcc aacgctacgg caagctccag caggccagcg
    37081 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    37141 ccacccgcaa gctggaaaac ctgactgata ttgaacgcca gctctcgacc cgcaaaccgg
    37201 ctggaaattt ctcgccggat acgccgcacg agggtgaaaa gcccgcgcca tccacccatg
    37261 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    37321 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    37381 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    37441 ctgcggatgg atgaaatgga cggtatgcag ctgtttgctg aaatccagaa agtgcagccg
    37501 ggaatgccgg taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    37561 cagcagggcg tttttagttt cctcaccaag cctgtcgaca aagacgcgct atatcaggca
    37621 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    37681 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    37741 gacgtcagcg tgttgattaa tggccagagc ggcaccggga aagagatttt cgcccaggct
    37801 atccacaacg ccagcccgcg taacagcaaa ccatttattg ctattaactg tggcgcattg
    37861 cccgagcagt tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    37921 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gtacgctatt tctcgatgag
    37981 attggcgata tgcccgcacc attgcaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    38041 gtgcgcccgc tgggcagcaa ccgcgatatt gatatcgacg tgcggattat ttccgccact
    38101 caccgcgacc tgccaaaagc gatggcgcgc ggggaattcc gtgaagatct ctattaccgc
    38161 ctcaacgttg tcagcctgaa aattccggct ctggcagagc gcacagaaga cattccgctt
    38221 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    38281 tccactgatg cgatgaaacg cctgatgacc gctagctggc cgggtaatgt gcgccagttg
    38341 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggttattag cgatgcatta
    38401 gtagagcagg cgctggaggg tgaaaacacg gcgctgccaa cctttgttga ggcacgtaat
    38461 cagtttgaac tcaactattt gcgtaagctg ctgcaaatta ccaaaggcaa cgtcacccac
    38521 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    38581 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    38641 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    38701 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    38761 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    38821 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    38881 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    38941 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    39001 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    39061 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    39121 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    39181 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    39241 ttagcgcgat cggcttcgct cggctgacga taccaggtgt gcgcggtaaa gcgcggcagt
    39301 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    39361 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    39421 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    39481 ataaagaaat cacctgccgg agcaaccagt ttcacgacat cgccaacatt ggcgtgattg
    39541 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    39601 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    39661 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    39721 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    39781 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    39841 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    39901 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    39961 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    40021 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagtgcttca
    40081 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    40141 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    40201 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    40261 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    40321 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    40381 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    40441 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    40501 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    40561 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    40621 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    40681 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    40741 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    40801 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    40861 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    40921 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    40981 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    41041 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    41101 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    41161 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    41221 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    41281 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    41341 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    41401 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgctaaaaac gctaaagcga
    41461 tggtagaagt gttcctggaa cgcggctaca aagtggtttc cggcggcact gataaccacc
    41521 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    41581 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    41641 tgacctccgg tattcgcgtg ggtactccgg caattacgcg tcgcggcttc aaagaagcag
    41701 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    41761 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgttacccg gtttacgcat
    41821 aagcgaaacg gttaatctgt tgaaaaaagg cttccttcgg gaagcctttc tacgttatcg
    41881 cgccatcaaa tctgtcgtaa ctgcgcctca acatacaaat agccaattcc cagcacctgt
    41941 tgtgcgcggc ttaattgccc gaagccaatt tgcgtcgctt tcacgctcgg tccttcgtcg
    42001 cggtcgaacg ggttaaatcc cgtctggcga ataatagtat taagccagtt ctcaccaaag
    42061 gcacaactgc gaccgtacaa ccaaatctga ttaatattga ggatgttgag gaagttatac
    42121 agactcaaac caatggcatt ggcactgcgg tcaacccagc tggtgatcca cggttctcca
    42181 ctctgccagg cagcgattaa ctgcgcggta gtcaattttt caggatcaag ctgagtatta
    42241 accggttgtg attttagcca tacccgcgcc tgttttttta atgcgcttaa cgaggcgacg
    42301 gtttccaggc agccgtaacg tccacagtcg cagacgacgc catccggatt aacgatggtg
    42361 tgaccaatct gtccgctacc atacaaactg ccgcgataaa tttgctcgtt aatcacgaac
    42421 gacgagccaa tgccgtaatc aacgttgatc acgcagaaat cccgttcctg cgaattattt
    42481 tgccattttt ccgccagcgc cagcatcacg cagtcattat cgaccatcac ccgaatgccc
    42541 agcttctctt ccagcagata cttcacctca accggcgttg tccacggcgc ttgcggcatg
    42601 gtttgcgaca ctcctgtcac tggatcaacc tgaccgtgga ttgccagcgc cagattgatg
    42661 gtgcggtccg gccacaattt acggtggcga tgccagcatt tttcgatttc ggacagcagc
    42721 gcctgcggcg tcggtacatc aatctgtaaa tactcaaatt cgcctttcgg acttaaacaa
    42781 gcattagcaa cctgacactc aatactggtg ggcgtcacgt tcaggcacag cgtccagtca
    42841 ccttccggcg caatcagcca tgtaccgcta ctatgcccgc gcgtctggct ttcatcgtcg
    42901 atattcacca cccgtttttc gctttccagt tcctgcaaaa tattactgac tgccggaatc
    42961 gaaatttgcg ccagccgggc cagcgttgat ttattggcgc gcttttgccg gtacagcagt
    43021 tccagaatca cgcatttgtt atggtggcga atctgttgat tgttaatgca ggctctcatc
    43081 ttcactaaac cttgtttagt taattgcgtg attattccgc gaaaacattg ccagattgtt
    43141 caatacactg ccacaaatct tttaattcag tatgtcttgt taatattgag ggcaccatga
    43201 ctccagtaaa agtgtggcaa gagcgcgttg agatcccgac ctatgaaacc gggccgcagg
    43261 atatacatcc catgttcctg gaaaatcgcg tttatcaggg atcgtccggc gcggtttatc
    43321 cctacggcgt gaccgatacg ctgagcgagc agaaaaccct gaaatcctgg caggcggtgt
    43381 ggctggaaaa cgactacatc aaagtgatga tcctgccgga actgggcggt cgtgtgcatc
    43441 gcgcatggga taaagtgaaa caacgcgatt ttgtctatca caatgaagtc attaaacctg
    43501 cgctggtggg gctgctggga ccgtggatct ctggcgggat tgagtttaac tggccgcaac
    43561 accatcgccc gaccaccttt atgcccgttg atttcaccct cgaagcccat gaagacggcg
    43621 cacagacggt gtgggtcggc gaaacggagc cgatgcacgg tttacaggtg atgacaggtt
    43681 tcacactgcg tcctgaacgg gcggcgttgg aaattgccag ccgcgtctat aacggcaacg
    43741 ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggg gaagggcatc
    43801 agagcgtctt cccgccggat gtaacggcgg tgtttgatca cggcaaacgg gccgtctccg
    43861 ctttccccat cgccaccggc acttactaca aagtggacta ctccgccgga gtggacattt
    43921 ctcgctataa aaatgtgccc gttccaacct catatatggc tgaaaaatca cagtacgatt
    43981 ttgttggcgc gtggtgtcac gatgaagatg gcggtttgct acacgttgcc aaccaccata
    44041 tcgcgccagg taaaaaacag tggagctggg gacacagtga attcggccag gcgtgggaca
    44101 agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt tttgccgata
    44161 accagcctga ttttacctgg cttgatgctt acgaagagaa gcgttttgag cagtatttcc
    44221 tgccttatca ttctttgggc atggtgcaaa atgcctcccg cgatgcggtg ataaaactcc
    44281 agcgtagtga gcgggggatt gagtgggggc tgtatgccat ctctccgttg aacggatacc
    44341 gcctggcgat ccgcgaaatc ggcaaatgca acgcgttact tgatgatgcc gtggcactga
    44401 tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgaaagg ctgaccattg
    44461 agctctccga tgccgacggc aatattgtac tgagttatca ggaacatcag ccgcaagagt
    44521 tgccgttgcc ggacgtcgcc aaagcgccac tgtcagcaca agacattacc agtacagatg
    44581 aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt tcaccgttcg
    44641 attactacct gcgcggcgtg gcgctggatc cgctggatta ccgctgtaac ctggcgctgg
    44701 cgatgctgga atataaccgt gccgatttcc cgcaagcggt ggcgtatgcc actcaggctc
    44761 tgaaacgcgc acatgcgctg aacaaaaatc cgcagtgcgg acaggcgagt ttgattcgcg
    44821 ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc tggcgtgcgg
    44881 tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg gcggcgcgta
    44941 atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc tgcccaacca
    45001 atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt caggacaacg
    45061 cgcgtgtgca gcgcgagaaa ctgctgcgcg attatccgct gaacgccact ctgtggtggc
    45121 tgaactggtt cgatggtcgt agcgaatcag ctctcgcgca gtggcgcggt ctgtgtcagg
    45181 gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga atgcccactc
    45241 tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt tatctacaag
    45301 ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt gatgtcttcc
    45361 cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag agtattgaag
    45421 agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc agctacaaca
    45481 aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc gacggctggc
    45541 gcgggttagc gatccatgcg tggaataagc aacacgatta tgagctggcc gcgcgttatc
    45601 ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa cgggatttgc
    45661 ttgataagtt aagcggagcc acaccggaga aacgactggc gcgtctggaa aataatctgg
    45721 aaattgcgct gaaacgcgac gacatgacag cagagttgct caatttgtgg catctcacgg
    45781 gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg tgggaaggcg
    45841 gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc tggcagcacc
    45901 ttgatgccag acagccgcag caggccagcg aactgcttca tgccgcgctg cattatccgg
    45961 agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg ttctggcagg
    46021 cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgat gcgttgttta cgtctggcgg
    46081 cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg gttgattatc
    46141 tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acacaccgca cagcaactgt
    46201 ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag gcggatttct
    46261 ttgctgtttc acaacctgat ctgttgtcgc tgtatggcga tttacaacag cagcataaag
    46321 aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt gcgcaatatg
    46381 agtctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg gcattattca
    46441 ccactgtgat gccttttatt tttaactacg ttcactaaac cctattaact gtaatatttg
    46501 agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt aataaaaacc
    46561 ctacaggagg ctctatgcct aaaaaaatga gaacaacccg taatctatta ttaatggcta
    46621 cgctattagg tagcgcttta tttgccaggg ctgcggaaaa agaaatgacc attggcgcaa
    46681 tataccttga tactcaggga tattacgctg gagtgcgtca aggcgttcag gatgcggcaa
    46741 aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat atttcgaaag
    46801 aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt ttatcggcag
    46861 tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt attccggtga
    46921 tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg gcgtatctgg
    46981 tcggcgatcc gctggaattt ggtaaaaaac tgggtaatgc tgccgccgat tattttattg
    47041 ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt gaagtttgtg
    47101 tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc gcgcaaattg
    47161 tcgctaatca ggaagggact gttttagata aagcgatttc cgttggtgaa aaactaatta
    47221 tctccacgcc ggatctcaac gccattatgg gtgaatcggg cggagcgaca ctcggcgcgg
    47281 taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt tcggatatga
    47341 ccactgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta gtggatattt
    47401 ccggtaagaa aatgggcaat gctgttctcg cgcaaacatt gaaggttatc aataaacaag
    47461 ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg gaagatggta
    47521 aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc agaggtccct
    47581 atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa taagcgttac
    47641 cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga agttcgtgcg
    47701 ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac cggcagtgaa
    47761 cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga cgaagctacg
    47821 ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggagtt aagtctggtg
    47881 gaagggctga cggtggcgga aaacctctgc ctcggtcagt ggccccgccg caacggcatg
    47941 attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct gggcgttgac
    48001 gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact ggtggaaatt
    48061 gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacctac gagttcgctt
    48121 gccagtgcgg aagttgaact ggtgatcagc gcggtgaaaa agatgtcggc actgggcgtg
    48181 gcggtgattt atgtcagcca ccggatggaa gaaattcgcc gcattgcctc ctgtgccacc
    48241 gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc cacgcatcat
    48301 attgtgtcgc taatgctagg gcgcgatcac gttgatattg cgccggtagc ccctcaggaa
    48361 attgtcgatc aggccgtgct ggaagtccgt gcattacgcc ataagcccaa gctggaggat
    48421 atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct gggggcaggg
    48481 cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg cgaaattgtt
    48541 atcaacggcg agaaaatcat gcgccccgat tacggcgaca tgctgaaacg cggcattggc
    48601 tatacgccag aaaaccgcaa agaagcgggg atcattccct ggctgggcgt tgacgaaaat
    48661 acagtgctga ccaatcggca aaaaatcagc accaacggtg tgctgcaatg gtccaccatc
    48721 cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggctgccag tagcgaaaca
    48781 cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg ttgggtctat
    48841 gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat cgaagccaaa
    48901 cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt ggtgtttatc
    48961 tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt acagcacggc
    49021 acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc cgccattctg
    49081 tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc attaccattg
    49141 ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa tgagatcggt
    49201 ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc tggctttatc
    49261 tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat tgccgcctgg
    49321 gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc gatggtggct
    49381 tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc gattgcttgt
    49441 ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct gcgcggcgtg
    49501 tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg cggaatgggg
    49561 ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct ggactggctg
    49621 ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgattgtgtt gtttgcgctg
    49681 tttgtgttca tcagccgcaa aaccgccttc gggcgctcgg tttttgccgt tggcggtaat
    49741 gctacggcgg cgcagttgtg cggcatcaac gttcgtcggg tgcgcattct tatctttacc
    49801 ctttcgggat tattagcggc ggtgaccggc attttgttgg cggcgcgcct cggttctggt
    49861 aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt ggtcggcggt
    49921 acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt gctggtgatt
    49981 acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca gcaggtggta
    50041 cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca gcgaagcagt
    50101 aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg aaaattaatc
    50161 gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca ggaaattcaa
    50221 ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta ctgatcaaaa
    50281 tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa caccgtgcca
    50341 cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat gaaccgtgcg
    50401 ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac cgcgtgctgg
    50461 tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt cctatctctt
    50521 gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat gccgaatatc
    50581 tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac gaagatggtg
    50641 cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc gaagtttccg
    50701 gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg atgatgctgg
    50761 cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa cgtctggcga
    50821 tggcaaaaca gttaggggtg atggatcacg gctatttagc gaccaccgaa ggtctgccgc
    50881 agataattgc tgaacttacc cacggtggcg cggatgttgc gctcgattgt tccggtaatg
    50941 ccgcaggtcg cttgctggca ctgcaatcca ctgctgactg gggacgggtg gtttacattg
    51001 gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat caacggcgga
    51061 ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccat gatctgacgg
    51121 actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa caggcaggtg
    51181 atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac ttcccggatt
    51241 aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc tggacgtttc
    51301 tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct tacgtcctgg
    51361 taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat ttgcaaaccg
    51421 ggtgagcggc aatcgttttg tctggcaggg gcgtgagtat caactgcaac cgaatgttga
    51481 gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat gtgtctcgca
    51541 cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc actatcgggt
    51601 aagtcaggcg tttcatttaa cggcggatac gctgatggtg acgctctctg tcaccaatca
    51661 aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt tgtcgccgca
    51721 aacgcggatt caggcacagg cgagcggtta ctggctggaa cgggagcagt ggctggcggg
    51781 tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc cgttgccgcg
    51841 ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg agcagccgca
    51901 ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt tcatctttgt
    51961 ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg aaccgatgag
    52021 ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc tagcaccagg
    52081 ggaatcaaca acctcagaga tgtcgttgcg ggtagagtgg ctgtaattta taaaatgccg
    52141 gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag aaattgcccc
    52201 cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct tccagaaatt
    52261 aatcagattt ggcccaaccg catcgccggt catatcccag taatggtgac caatcaccgc
    52321 cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa agccaagcac
    52381 gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca gcatcggcat
    52441 tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca ttttgggaaa
    52501 accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca aaacggggcg
    52561 ggcagctcca aaatcaaaat aacgtagtgt gttcataacc aatccctttg caagataatg
    52621 tgatttaaac gtaatacaca ctcttgcacc tcgccacgcc ccaggcattg agatattcgc
    52681 taattatcct ggttacagtg atttaagcgc gatgttctca tctatcagca gtttcgcatc
    52741 aaacgttttg ccgctctgga tccatttgcg aattgggcga atctcacgcc cctgattcag
    52801 cgtgaccgca ccgataagca cgccgttttg cagattaaac caaatcgcct tctgagtttc
    52861 cgggttgcca cgacaaagcc agtcatcgcc acgcatatcg ccaataaact gtaagttatc
    52921 actgtactga tcgctccaga accacggcgg cggcagtagc ggtagcggta gccctaacat
    52981 tgcggcagcg gcaatttgcg cctggttatt ggcgttttcc cagctttcgc agcggtgtag
    53041 tgcaccatta tcaagacgag tgattgccac atcgccaccg gcaaagatcg cgggatcgca
    53101 ggtgcggcaa gcctcatcaa tgacaatgcc attggcagta tcaaggttgg cctcgcgagc
    53161 cagttgctcg ttggcgctga taccaatacc gtaaatcacc acatcagcct gaagcgtttc
    53221 cccactttgc agcgtcagtt ctactttttc accatcgacc acatgttcaa tggcattatt
    53281 gagcagaatg cgcacaccag cctgctggtg gcgctgtaaa agatagcgtt gcacgggcgg
    53341 tggtgcatta cggcccatga cggttgccgc cagttcaatc actgtcacct tacatctgcg
    53401 ctgcgtggcg ctggcagcca gttccagacc aatagttccg gcaccgacaa tcacgactga
    53461 ccgttcgggc tgcagaactt ctcgcagtct ggcggcatca ccggcatggc gtagggtaaa
    53521 gcagcgttct cccagtgcat caagcaacgg cagcggtcga gctgccgcgc cggttgctat
    53581 aaaaagctga tcccagtgcc agctttcgcc gttggttaac actaactctc gtgtgtcgcg
    53641 acccagcgtt ttgatggtta caccggaatg cagatgaaca ttgttttcct gccaccagtt
    53701 agcgggtaac acctgctgta actgcgggga atcttccagc aacatggatt tcgagagtgg
    53761 aggtcgttca taaggaagat gtcgctcatc ggaaaacaga tgcagctcac cggtgaaccc
    53821 ttgctggcgt agcgaggccg cagccattgc cgccgcttgc ccgccaccga caataatgat
    53881 ggttttttct ttcatcgtgc ttccttaaag atccagccca gccgctacgt ggcgaatgcc
    53941 gcgaatcgct aaacccgcat cagcgttgat catcacaccg cttaatgcgc gattattgcg
    54001 ccgcgatgcc aacatcacat acggacccgt aaaatccgcc ggttgcggga aaaattgcag
    54061 cggtaaaatg gcggcaattt tctccggcgt cagagactgc attatcgagg tttcactttg
    54121 cccgagcgcc tgtgggccgc gcaggtcgct ggccatacca cacgggccga cgccattcac
    54181 ccgcactttc ggtgccagtt cataagccag ttggcgaata agtccggttg cggcatgttt
    54241 actggcggtg tacagcgggc caccgccgcc aggataccag gcggcatttg acagtgtgaa
    54301 aatcatgctg ccttcactgg cgattaacgc cggagcgcag gcttttgcgc ccagcaggta
    54361 accgagtacg ttgacgttaa acagctcgtg gaagccggtt tcgagcgtct ctgcgggagt
    54421 attaaccagt gaggcattgt gatcccagat gcctgcattg ccgataaaac aatccagctt
    54481 gccggaacga gtcaggatct gatcgaccgc gcgttgataa tcggcataac aggtcacgtt
    54541 accttccacc gccagaatat gttcgccaaa tcgctgacgc agactggcga cttttgccgc
    54601 cgacagttcc agcgtggcaa cctgcgcgcc ttcttcgata aatcgctcga ccagcgccag
    54661 ccctaatccc gatccgccgc cggtaataaa aatggactcg ttatgcagat cgctcatggc
    54721 tgcgcctccg gtaagtcgat gaaaatgtca ccaccttcaa cgtgtactgg ataagtggtg
    54781 agcggatcgg tggcgggcag gcataacgct ttccccgttt tcaggcaaaa actggcggcg
    54841 tgtagcgggc actccaccgt ggcgtcatct tccagatacc cttctgacat cgacgcatta
    54901 ccatggctgc aacgatcgtt aatggcataa aactcgccgc caacgttgaa cagggcgatg
    54961 acgggcgagg tatcgatccg gagagcctca ccctccggca catccgcaac gggacacgca
    55021 taaattcgat tcatcagaac agtacactca ggttatggga agtgattacc gcttgatcca
    55081 ggacgatatc ccgttccagc aagcgccagt tgtcatcttc cagacggcga actttgtcga
    55141 aacgcgttcc aacatagaat gtttcatcgc gctctttttg tgcccgataa agcagataat
    55201 ttacccgcac agcaaatacg tttgggatgt cggtttcgct tatctggcag ttgctgatta
    55261 agtgacgggt gcgtgacggc ggctcttctg cccaggccat gcccgtttcc agacgggcga
    55321 ttcgccgctc cagctggtct ttggtgtcat taaaaatcca ggttgtcggt ggctggacgc
    55381 ctttgcggcg gtcgcgtgtt tgcgcgttaa ctgtggtgcg catggtgtaa cgaatctctt
    55441 cgtcgagctg cgccagccag tcacgaaatt tccagtcgtc cagtaagctg gcttcgtgaa
    55501 agagaaactg gctaatgcgg tgatgtaact ctagtgaaac ttgcgcactc atttcatcac
    55561 ctcctgctgg taagcagcgg ttttatcgag cacttcctgc cagctttcgc tactcagaag
    55621 atcggcccag cgttggtaca ttccacgagc ggcagtttct gaaaagatat agttagtaat
    55681 gccaggaatg ccgtcgtcgc ggcgcttttc ctgaccaagc cccatttcca gacacagttt
    55741 gctgttgcgg gcgcggtggc ctttaagcaa tttctggatt tcacaccagt tctccgagtc
    55801 atcctgctcg agaaaaccag caggaccaaa agcacgagtg gcgctgtttt caaaagcggc
    55861 tttaacttca tcggaggcgg ctttgtcagt aatacagaac gcccacactt caacttgatc
    55921 agggccgcgc ggatgccaga cgcggagtgt ggcagtgccg tttagccatg aaagcgtggg
    55981 gaatatattg ttatgacccg ccaggcgcag ggcgcgaact tcacctaaac gttgttctgc
    56041 ttcggcatag gtttcgcggt aatagcttga aactgcgcca tcgacccaga cattagcatc
    56101 cggtttttca gtaaagaaga aaccgctacc gtgaccgtcc tgaccaaatt gcagcgcatc
    56161 tttggcggtt tcccacaccg ggcgggcggt ttgtccatca ccgaggcgct tatcgctgcc
    56221 atcatctttc gcccctaata cctgaacggc agaagcatgg ctgaacagag catgatactg
    56281 gtcactggcg aactgctctg ccgggaattt ccagttacag ttgatcaccc acttttgtac
    56341 gccgccgaca atttcggtgc cgccttcgcg acgatccagc atgccatcca gataccaggc
    56401 aatgtcaccc aggtaatcac gcaggcccgg tgcgctggta tcccagttgc caaaaatcag
    56461 ccctttataa ctctccacac aaggaacttc gtttagtccc cagtgggatt tacacaaccc
    56521 ttgtgggtag gcgcgaggtt ccagcggtac atcgatcaac tcgccgttaa tgccataaga
    56581 ccagccgtga tacgggcagg taaaggcgcg agtgttgccg caatctgcat aactcacacg
    56641 catggcccgg tggcggcatt ggttgagaaa cgccttgatg ctgccgtctt tctgacgcac
    56701 tacgacaacc gcatcttccc ccatgtaggt gttaaagaaa tcaccaggtt ttgggatctg
    56761 gctttcgtgg gcgagaaata gccagcaacg gccgaaaata cgttcaagct ccagttggta
    56821 aatatctggg tcggtataaa tacgcggagt gacccgacca ttttgggtat caatcagttg
    56881 gtaaatgttc aaatctgagg gtgtggtcat attttttaat cctgaaagat aagatgtatt
    56941 gaccttctat ttttgtgcca taaggatgtt gctggttggt tgctaatatg tgaaatagtt
    57001 aatttctacc agctgagacg ttttttcgat aacaagggga aggtgatgga actacggcat
    57061 ttacgctatt tcgtcgcagt ggcgcaggca ctgaacttta cccgtgcggc ggaaaaactg
    57121 catacctcac agccttcgtt aagcagccag atccgcgatc ttgaaaactg tgtcggtgtt
    57181 ccgttactgg tgagggataa gcgcaaagtc gcgttgacgg cggcgggaga atgttttctc
    57241 caggacgcgc tggcaatcct cgaacaagcg gaaaatgcca aattacgggc gcggaaaatt
    57301 gttcaggaag acagacaatt aaccattggc tttgtgccat cggcggaagt gaatttactg
    57361 ccaaaagtat taccgatgtt tcgtctcaga cagccagaca ccttgattga gctggtgagt
    57421 ttaatcacca cgcaacagga ggaaaaaatt cgccgtggtg aactcgacgt cggcttaatg
    57481 cgccatcctg tttatagccc ggagattgat tatctggagc tttttgacga accattagtg
    57541 gttgtgttac cggttgatca ccctttagca catgaaaaag agatcaccgc cgcgcaactg
    57601 gatggcgtga atttcgtcag taccgatccg gcgtattccg gttcgcttgc tccgatcgtt
    57661 aaagcgtggt ttgcgcaaga aaacagccag ccaaatatcg tccaggtggc aacgaatatt
    57721 ctggtgacca tgaatctggt gggcatgggg ctgggcgtca ctttgatacc cggttatatg
    57781 aataatttta ataccggaca ggttgttttt cgtcccattg ccggaaatgt gccttccatt
    57841 gctttactga tggcgtggaa gaaaggggag atgaagccgg cattacgcga tttcatcgcc
    57901 attgtgcagg aacgtttggc aaacgtaacg gcataattac gcattatttt tgcaacatgc
    57961 gtcgcggatc gcagtttttt agtgcttgtg aactggcgtt acagacatcg ggaagcaggc
    58021 tcgaatattc ttatttttca acttgcacca acattcgatt aaccccagac taacgcctcc
    58081 tgacgggagg gactcatggt tttgcaatcc acgcgctggt tggcgctcgg ctatttcaca
    58141 tactttttta gttacggcat ttttctacct ttctggagcg tctggcttaa agggattggt
    58201 ttaacgccag aaaccatcgg cctgttattg ggggcaggtc tggttgcccg ttttctcggg
    58261 agtttgctca tcgcgccccg cgtcagcgat ccttcccgcc tgatttccgc cttgcgcgtg
    58321 ctggcactgc tgacacttct ctttgctgtc gccttctggg cgggggcgca cgtagcgtgg
    58381 ctgatgctgg tgatgattgg ctttaacctc tttttctcac cgctggtacc gttgaccgat
    58441 gcactggcga atacgtggca aaagcagttc ccgcttgatt acggcaaagt gcgactgtgg
    58501 ggctcggtgg cgtttgtcat tggctcggcg ctgacgggca aactggtcac tatgtttgat
    58561 tatcgggtga tcctcgcgct gttgacgttg ggcgtggcat ccatgctgct cggctttctc
    58621 atccgtccga cgattcagcc acaaggggca agccgccagc aggagagcac cggttggtca
    58681 gcgtggttgg cgctggttcg ccagaactgg cgctttctgg cctgcgtttg tttattgcag
    58741 ggggcacatg cggcctatta cggttttagc gccatttact ggcaggcagc tggctactcg
    58801 gcctcggcgg tggggtattt gtggtcgctg ggcgtggtgg cggaagtcat tatctttgcg
    58861 ctgagtaata aacttttccg ccgttgtagt gcacgcgata tgctgttgat ctcggcgatt
    58921 tgcggcgtag tgcgctgggg cattatggga gcaactacgg cgttgccgtg gttgatagtg
    58981 gtgcaaattc tgcattgcgg caccttcacg gtctgccacc tggccgccat gcgttatatt
    59041 gctgctcgcc agggtagcga agtcatccgt ttacaggcgg tttactctgc cgtcgcgatg
    59101 ggcggcagta tcgctatcat gaccgttttc gccggtttcc tgtatcaata tctgggccac
    59161 ggcgtgttct gggtaatggc gctggtggcg cttccggcaa tgtttttgcg cccgaaagtt
    59221 gttccctcat gctgattcga gcattttgcg gatctgctcc tgttgatgtg ttgtcagcgt
    59281 caggtccgta tagatcagcg gtggtgaaaa gagcggtaat ggcatggtat aaggcgcaat
    59341 aatcagtgcc gcgccacgcg gagcgcctgt ctgcaaaaat gcctttaccg acatatgctt
    59401 aatgttgagc ggcagtaacg ttagttcgcg taactgctgc tcaatctgcg cttctcgctc
    59461 gctatcattc ccggtaagaa gaataatctg tttttcatgc agatcgtttt cctgcattag
    59521 ccaggcaccg aaaatcaccg cgaccagacc actttcctca tcagaaagat ggacgccgta
    59581 ttcactttca aatccggcca gcgccgcgcg ggtggtgcgc accaggcgtg ggtacagtct
    59641 ggcgaactct tccggcaggg tattatcaat accgatggca aacaaactgc gatttaacgc
    59701 ctgggcgagg tgggtataaa gctgatcaca taacccctgt tcatcataaa aacgaacatt
    59761 tcccagctca cgaaaatggt ttaccagacg tttgatagac tgacgcaatt gtctgtctcg
    59821 ctgatgcgca tcccgcagtg gatcgggaac ccgcaacatg gaaaaaagta gtgccataaa
    59881 cagtggctca tcaggtggta caggctggag cgcccgacgc tgccagtggc gtccaatttc
    59941 ctgcgctacc tgaaattcaa ggcaggattc cgcccagcga cgttggagcg gattaaactg
    60001 aggcgtaatt ccagcgtggt gttgcagcaa acaatattgc agatacagac acaggaaatg
    60061 aatatcgcgc gtttcaaagc gtttttgcag ccgtcgggag cagagattca ctaacgcttg
    60121 tagattggta tcgtcgtaaa agttacgcgc aattccgcgc cgcttcagtt cactcttcag
    60181 ggcgggggtg aaatggctgg taataaatga tggacacaga cggaaaccac gtcgtagcca
    60241 gtgaaataaa cacaaacgtt ggttaagcac tgtaccttca acccggtagc taccgtcata
    60301 accagccgtc agtgtgagtt gatgatagcg caggatctcc tgtcctgttt cgctgatatc
    60361 aagactggca atatcatcat ccacaccatt aagctcgctg aaagttgccg tggtggcggt
    60421 caaccccggc tgaaagagcg tcagcaagat ctggcagcgg cgctggggag ccgaaaggac
    60481 agatggtgga gcaagcgtag gcatcatctg tagatctccc ataagcataa ttgataagaa
    60541 tagctaaagt ttgctcaggg aaatgacgaa gggaatgcta atcatcagaa atgttggcaa
    60601 gcgtcacaga atttttctca tcttaaactg ttctgaccag tggattagca tgtcttaagg
    60661 tggttttgtg aatgattatc gtgctagcga tcatgggtta gttctgcgga ttcaacgcgc
    60721 taaggataaa gtccaccatc tgttcgcgat aaacatcgcc gtggcgatcg gaaaaagcat
    60781 caatgtgttc gccatccggg attaggatca accgttttgg ctcttttgcc aggctataca
    60841 acttttcgct gtgctgccat gggataacgt gatcagcttt accgtgaatg agtaaaagcg
    60901 ggatcgggct gacgctggcg atataatttt cgccgctgta actctcatca agtaagtagc
    60961 cactgccggg gatcatctgg ttggcgatgg ttgcataaga ggcaaatgtg gagtcgagga
    61021 tcaccgcacg tatgccttca cgatcaccct gaccaataac agcaagaata ttcgccccgc
    61081 caatactctg cccgaacagc accagccgtt gtgggtttac atcactgcga tggcgcacca
    61141 cattgatggc gctttgcgta tcgtccagca atccggcctg ggacggcgtg ccttttgatt
    61201 taccaaaccc gcgataatca aacataaaaa cgttgaaatt acgctcgggt aaccaactga
    61261 ccagcggcca gtgggcggac atatttccgg cattgccgtg agcatgaatg atggttgcga
    61321 tggcgttgtc agcagggccc gtcgaagaag gaataaacca gccttgcaga cgagtaccgt
    61381 ccttagccgt aaattcgacg gattccgccg accaggggtc gggaccgtaa attttatcgt
    61441 caggataata gaagaagttg atggcaacgc gcggcactaa ataaacgcag aaggcaacaa
    61501 caaagagaat aaacagaatt ttgggaacgc gtttgttcac tggcagtgcc ataacctttc
    61561 cttgcgactt ctttatcatc cattaatcgg ggaaagatgg tgggagagtg acgaaagaaa
    61621 gtaaagcggt gaggaagtat ttcagaatag cgcctgaaag gcgagggcgg gtgagtgaca
    61681 tcacccgcct gagtcattaa cgcttcagag cgtcgcttaa ctcgtcacgc atgttcgcca
    61741 gcatggcttt cacaacacgt gggttaccag caacgatgtt accggtcagc atatagttat
    61801 gaccaccggt gaagtcgctg acgatgccgc ccgcttcacg aaccagcagc tcgcctgccg
    61861 cgaaatccca cggacgcaga ccgatttcaa agaaaccgtc aacacgaccc gcagcgacgt
    61921 aagccagatc cagcgccgca gaaccggtac gacggaagtc tgcacattcg ttgaacagtt
    61981 tgccgacgat gttgatgtag gtagtggcgt actgttttgc tttgaacggg aagccggtcg
    62041 ccagaatggt accgtcgaga tcgcgagcgg tgctaccgcg cagacggtag ccgttcagct
    62101 gtgcgccctg accgcgagtg gcggtgaaca gttcgttacg cataggatcg tataccacag
    62161 caacttcggt gcggcctttg atacgaacgg cgatagatac cgcgaagtgc ggcagacgtt
    62221 tgataaagtt ggtagtacca tccagtggat cgataaccca ttgaacatcc tgatcagtac
    62281 cttcaagttc accgctttct tcggtgatga tggtgtgctg tgggtaagat ttacgaatcg
    62341 tgtcgataat caccgcttcg gcagctttat ctacgttggt cacgaaatcg ttactgcctt
    62401 tctggctcgc ttctacagcg tccggggttt catagttttt ggcaattaaa ttacccgcct
    62461 tgcgcgctgc gcgcacggcg atgttcagca tcggatgcat cggtctctct cactggatgt
    62521 taaagaacgg gaaaacggcg cagagtatag cagcgagatc ggcatctgtc ttcgttttat
    62581 gataagatgc gtgaataatc tttagatgaa gaaagacaat gctgcaaaat attcgaattg
    62641 tgctggtgga gacgtcacac accggcaata tgggttctgt tgcccgtgcc atgaaaacaa
    62701 tgggattaac caatctgtgg ctggttaatc cactggtgaa acccgactcc caggcgattg
    62761 ccctggcagc aggggccagc gatgtgattg gtaatgctca catcgtcgat acgctcgacg
    62821 aagcgttagc cggttgtagc ctggtggtgg gcaccagcgc acgctcccgc acgctgccgt
    62881 ggccgatgct cgacccgcgc gaatgcggtc tgaaaagcgt cgctgaagcg gcaaataccc
    62941 cggtggcgct ggtgtttggt cgtgagcgcg tcggcttgac caatgaagag ttgcagaaat
    63001 gccattatca tgtcgcgatt gcggctaacc cggaatacag ctcgctgaac ctggcgatgg
    63061 cggttcaggt tattgcctat gaagtgcgca tggcctggct ggcaactcag gaaaacggcg
    63121 agcaggtcga gcatgaagag acgccgtatc cgctggtcga tgatctggag cgtttttatg
    63181 gtcatctgga acaaacgctg ctggcaaccg gttttatccg tgaaaaccat ccggggcagg
    63241 tgatgaataa attgcgccgt ctgtttaccc gtgcgcgccc ggaaagccag gaattgaata
    63301 tcctgcgcgg gattctggct tctattgagc agcagaataa aggtaataag gcctgagggc
    63361 taagccagat aaatgcacgg acggtcccct cgccccttcg gggagagggt tagggtgagg
    63421 ggaagaggcc agcgctaatg cgaataattg ccctcaaccc gcccatggtc tggaaggaca
    63481 acaggtacaa acgccacgat aaaaaaatgg cactgaaggt taaatacccg actaaatcag
    63541 tcaagtaaat agttgaccaa tttactcggg aatgtcagac ttgaccctgc tatgcaatac
    63601 ccccactttt acaataaaaa accccgggca ggggcgagtt tgaggtgaag taagacatga
    63661 gactgacatc taaagggcgc tatgccgtga ccgcaatgct tgacgttgcg ctcaactctg
    63721 aagcgggccc ggtaccgttg gctgatattt ccgaacgtca gggaatttcc ctttcttatc
    63781 tggaacaact gttttcccgt ctgcgtaaaa atggtctggt ttccagcgta cgtggaccag
    63841 gcggtggtta tctgttaggc aaagatgcca gcagcatcgc cgttggcgaa gtgattagcg
    63901 ccgttgacga atctgtagat gccacccgtt gtcagggtaa aggcggctgc cagggcggcg
    63961 ataaatgcct gacccacgcg ctgtggcgtg atttgagcga ccgtctcacc ggttttctca
    64021 acaacattac tttaggcgaa ctggttaata accaggaagt gctggatgtg tctggtcgtc
    64081 agcatactca cgacgcgcca cgcacccgca tacaagacgc gatcgacgtt aagttacgcg
    64141 cttaataaaa agaattcaga atcaggccgg ggcgtatcgc gctccgtaaa cacggtcgta
    64201 catccagccg gtagcctggt tccttgcatt gagtgatgta cggagtttat agagcaatga
    64261 aattaccgat ttatctcgac tactccgcaa ccacgccggt ggacccgcgt gttgccgaga
    64321 aaatgatgca gtttatgacg atggacggaa cctttggtaa cccggcctct cgttctcacc
    64381 gtttcggctg gcaggctgaa gaagcggtag atatcgcccg taatcagatt gccgatctgg
    64441 tcggcgctga tccgcgtgaa atcgtcttta cctctggtgc aaccgaatct gacaacctgg
    64501 cgatcaaagg tgcagccaac ttttatcaga aaaaaggcaa gcacatcatc accagcaaaa
    64561 ccgaacacaa agcggtactg gatacctgcc gtcagctgga gcgcgaaggt tttgaagtca
    64621 cctacctggc accgcagcgt aacggcatta tcgacctgaa agaacttgaa gcagcgatgc
    64681 gtgacgacac catccttgtt tccatcatgc acgtaaataa cgaaatcggc gtggtgcagg
    64741 atatcgcggc tatcggcgaa atgtgccgtg ctcgtggcat tatctatcac gttgatgcaa
    64801 cccagagcgt gggcaaactg cctatcgacc tgagccagtt gaaagttgac ctgatgtctt
    64861 tctccggtca caaaatctat ggcccgaaag gtatcggtgc gctgtatgta cgtcgtaaac
    64921 cgcgcgtacg catcgaagcg caaatgcacg gcggcggtca cgagcgcggt atgcgttccg
    64981 gcactctgcc tgttcaccag atcgtcggaa tgggcgaggc ctatcgcatc gcaaaagaag
    65041 agatggcgac cgagatggaa cgtctgcgcg gcctgcgtaa ccgtctgtgg aacggcatca
    65101 aagatatcga agaagtttac ctgaacggtg acctggaaca cggtgcgccg aacattctca
    65161 acgtcagctt caactacgtt gaaggtgagt cgctgattat ggcgctgaaa gacctcgcag
    65221 tttcttcagg ttccgcctgt acgtcagcaa gcctcgaacc gtcctacgtg ctgcgcgcgc
    65281 tggggctgaa cgacgagctg gcacatagct ctatccgttt ctctttaggt cgttttacta
    65341 ctgaagaaga gatcgactac accatcgagt tagttcgtaa atccatcggt cgtctgcgtg
    65401 acctttctcc gctgtgggaa atgtacaagc agggcgtgga tctgaacagc atcgaatggg
    65461 ctcatcatta atcggtatcg gaatcaggag aatttataat ggcttacagc gaaaaagtta
    65521 tcgaccatta cgagaatccg cgtaacgtgg gttcctttga caacaacgac gagaacgtcg
    65581 gcagcggcat ggtgggggca ccggcctgtg gcgacgtgat gaagttgcag attaaagtca
    65641 acgatgaagg tatcattgaa gacgcgcgtt ttaaaactta cggctgcggt tctgctatcg
    65701 cttccagctc cctggtcacc gaatgggtga aagggaagtc tctcgacgaa gcgcaggcga
    65761 tcaaaaacac cgatattgct gaagaacttg aactgccgcc ggtgaaaatt cactgttcta
    65821 ttctggcaga agacgcgatc aaagccgcca ttgcggacta taaaagcaaa cgtgaagcaa
    65881 aataagagtt gaggtttggt tatgtcgatt acactgagcg acagtgcagc agcgcgagta
    65941 aatacctttc tggctaaccg cggcaaaggg tttggcctgc gtctgggcgt gagaacctcc
    66001 ggatgttcag gtatggctta tgtactggaa tttgttgacg aaccgacgcc ggaagacatc
    66061 gtgtttgaag acaaaggcgt gaaagtcgtg gtcgatggca aaagcctgca atttctggac
    66121 ggtacgcagc tggacttcgt aaaagaaggc ctgaatgaag ggtttaaatt caccaacccg
    66181 aacgttaaag atgagtgtgg ttgcggcgaa agcttccacg tttgatgcgc ataccgataa
    66241 ccccaccgtg gtcgcctgcg cgtggggttt gttttacctg attcgccgtt atcgcggcgg
    66301 atcgcagccc tgagaatgtt atggattact tcaccctctt tggcttgcct gcccgttatc
    66361 aactcgatac ccaggcgctg agcctgcgtt ttcaggatct acaacgtcag tatcatcctg
    66421 ataaattcgc cagcggaagc caggcggaac aactcgccgc cgtacagcaa tctgcaacca
    66481 ttaaccaggc ctggcaaacg ctgcgtcatc cgttaatgcg cgcggaatat ttgctttctt
    66541 tacacggttt tgatctcgcc agcgagcagc atactgtgcg cgacaccgcg ttcctgatgg
    66601 aacagttgga gctgcgcgaa gagctggacg agatcgaaca ggcgaaagat gaagcgcggc
    66661 tggaaagctt tatcaaacgt gtgaaaaaga tgtttgatac ccgccatcag ttgatggttg
    66721 aacagttaga caacgaggcg tgggacgcgg cggcggatac cgtgcgtaag ctgcgttttc
    66781 tcgataaact gcggagcagt gccgaacaac tcgaagaaaa actgctcgat ttttaatttc
    66841 tggaagctaa acatggcctt attacaaatt agtgaacctg gtttgagtgc tgcgccgcat
    66901 cagcgtcgtc tggcggctgg tattgacctg ggcacaacca actcgctggt ggcgacagtg
    66961 cgcagcggtc aggccgaaac gttagccgac catgaaggcc gtcacctgct gccatctgtt
    67021 gttcactatc aacagcaagg gcattcggtg ggttatgacg cgcgtactaa cgcagcgctt
    67081 gacaccgcca acaccattag ttctgttaaa cgcctgatgg gacgctcgct ggctgatatc
    67141 cagcaacgct atccgcatct gccttatcaa ttccaggcca gcgaaaacgg cctgccgatg
    67201 attgaaacgg cggcggggct gctgaacccg gtgcgcgttt ctgcggacat cctcaaagca
    67261 ctggcggcgc gggcaactga agccctggca ggcgagctgg atggtgtagt tatcaccgtt
    67321 ccggcgtact ttgacgatgc ccagcgtcag ggcaccaaag acgcggcgcg tctggcgggg
    67381 ttgcatgttc tgcgcttact taacgaaccg accgctgcgg ctatcgccta cgggctggat
    67441 tccggtcagg aaggcgtgat cgctgtttat gacctcggtg gcgggacgtt tgatatttcc
    67501 attctgcgct taagtcgcgg cgtgtttgaa gtgctggcaa ccggcggtga ttccgcgctc
    67561 ggcggcgatg atttcgacca tctgctggcg gattacattc gcgagcaggc gggcattcct
    67621 gatcgtagcg ataaccgcgt tcagcgtgaa ctgctggatg ccgccattgc agccaaaatc
    67681 gcgctgagcg atgcggactc cgtgaccgtt aacgttgcgg gctggcaggg cgaaatcagc
    67741 cgtgaacaat tcaatgaact gatcgcgcca ctggtaaaac gaaccttact ggcttgtcgt
    67801 cgcgcgctga aagacgcggg tgtagaagct gatgaagtgc tggaagtggt gatggtgggc
    67861 ggttctactc gcgtgccgct ggtgcgtgaa cgggtaggcg aatttttcgg tcgtccaccg
    67921 ctgacttcca tcgacccgga taaagtcgtc gctattggcg cggcgattca ggcggatatt
    67981 ctggtgggta acaagccaga cagcgaaatg ctgctgcttg atgtgatccc actgtcgctg
    68041 ggcctcgaaa cgatgggcgg cctggtggag aaagtgattc cgcgtaatac cactattccg
    68101 gtggcccgcg ctcaggattt caccaccttt aaagatggtc agacggcgat gtctatccat
    68161 gtaatgcagg gtgagcgcga actggtgcag gactgccgct cactggcgcg ttttgcgctg
    68221 cgtggtattc cggcgctacc ggctggtggt gcgcatattc gcgtgacgtt ccaggtcgat
    68281 gccgacggtc ttttgagcgt gacggcgatg gagaaatcca ccggcgttga ggcgtctatt
    68341 caggtcaaac cgtcttacgg tctgaccgat agcgaaatcg cttcgatgat caaagactca
    68401 atgagctatg ccgagcagga cgtaaaagcc cgaatgctgg cagaacaaaa agtagaagcg
    68461 gcgcgtgtgc tggaaagtct gcacggcgcg ctggctgctg atgccgcgct gttaagcgcc
    68521 gcagaacgtc aggtcattga cgatgctgcc gctcacctga gtgaagtggc gcagggcgat
    68581 gacgttgacg ccatcgaaca agcgattaaa aacgtagaca aacaaaccca ggatttcgcc
    68641 gctcgccgca tggaccagtc ggttcgtcgt gcgctgaaag gccattccgt ggacgaggtt
    68701 taatatgcca aagattgtta ttttgcctca tcaggatctc tgccctgatg gcgctgttct
    68761 ggaagctaat agcggtgaaa ccattctcga cgcagctctg cgtaacggta tcgagattga
    68821 acacgcctgt gaaaaatcct gtgcttgcac cacctgccac tgcatcgttc gtgaaggttt
    68881 tgactcgctg ccggaaagct cagagcagga agacgacatg ctggacaaag cctggggact
    68941 ggagccggaa agccgtttaa gctgccaggc gcgcgtcacc gacgaagatt tagtggtcga
    69001 aatcccgcgt tacactatca accatgcgcg tgagcattaa cagaggttag tatgggactt
    69061 aaatggaccg atagccgcga aattggcgaa gcactgtacg atgcgtatcc cgatcttgat
    69121 ccgaaaacgg ttcgattcac cgatatgcat cagtggattt gcgatctgga agatttcgac
    69181 gacgacccgc aggcatccaa cgagaaaatc ctcgaagcga ttttgttagt ctggctggac
    69241 gaggccgaat aatttccgcg tcatgcttca cgccgcagat gcgttggctg cgctcgttca
    69301 ccccagtcac atagttatct atgctcctgg ggcttcactc acttgccgcc ttcctgcaac
    69361 gcgaatcatt tagcggaaaa atcctggggc tgccaactgg cggccctttt acaaagaagg
    69421 ataactaaaa tgacagaagc gatgaagatt accctctcta cccaacctgc cgacgcgcgc
    69481 tggggagaaa aagcaactta cagcattaat aatgacggca ttaccctgca tttgaacggg
    69541 gcagacgatc tggggctgat ccagcgtgcg gcgcgcaaga ttgacggtct gggcatcaag
    69601 catgttcagt taagcggtga aggctgggat gcggatcgct gctgggcatt ctggcaaggt
    69661 tacaaagccc cgaaaggcac gcgtaaagtg gagtggccgg atctggacga tgcccagcgc
    69721 caggaactgg ataaccgcct gatgatcatc gactgggtgc gtgacaccat caacgcaccg
    69781 gcagaagaat tgggaccatc gcaactggca cagcgtgctg ttgatctgat cagcaacgtc
    69841 gcgggcgatc gtgtgactta tcggatcacc aaaggcgaag atctgcgtga gcaaggttat
    69901 atggggctgc acaccgtcgg gcgcggttca gaacgttctc cggtattgct ggcgctggat
    69961 tacaacccaa ctggcgataa agaagcaccg gtgtacgcgt gcctggtagg taaaggtatc
    70021 acttttgact ccggcggcta cagcatcaaa cagactgcgt ttatggactc gatgaagtcg
    70081 gacatgggcg gcgcggcaac ggttaccggg gcgctggcat ttgccattac gcgcggactg
    70141 aacaagcgcg tgaagctgtt cctctgctgt gcggataacc tgattagcgg caatgcgttc
    70201 aagctgggcg atatcatcac ctatcgcaac ggtaaaaaag ttgaagtgat gaacactgat
    70261 gccgaagggc gtctggtgct tgccgatggt ctgattgatg ccagtgcgca gaaaccggaa
    70321 atgatcattg atgcggcgac cctcaccggg gcggcgaaaa ctgcgctggg taatgattat
    70381 cacgcgctgt tcagttttga cgatgcgctt gccggtcgtt tgctggcgag tgcctcacaa
    70441 gagaacgaac cattctggcg tctgccgctg gcggaattcc accgcagcca gctgccgtct
    70501 aactttgccg aactgaacaa taccggaagc gcggcgtatc cggcaggcgc gagcacggcg
    70561 gcgggcttcc tgtcgcactt tgttgagaac tatcagcaag gctggctgca tatcgactgc
    70621 tcggcgactt accgtaaagc gccggttgaa cagtggtctg cgggcgctac gggacttggt
    70681 gtgcgcacga tagctaatct gttaacggcg taaactaata tgccggatgc ggcgtaaacg
    70741 ccttatccgg cctacaaatt ctgcaaattc aacaaattgc gaatcccttg taggcctgat
    70801 aagcgtagcg catcaggcat ttgcgccccg aacgttgaat gaattgtggt tcatatgtcc
    70861 gaaacaaaaa acgaacttga agacctgctg gaaaaagcag caaccgaacc ggcgcaccgc
    70921 ccggcctttt tccgtactct actggaatcc accgtctggg tgcctggtac ggcggcgcag
    70981 ggcgaggctg tggttgaaga tagtgcgctt gatttacagc actgggaaaa agaagacggc
    71041 accagcgtca ttcctttttt cacctcgtta gaagcgcttc aacaggcggt tgaagacgaa
    71101 caggcatttg tcgtaatgcc cgttcgcaca ttgtttgaga tgacccttgg cgaaacgctc
    71161 ttccttaatg ccaaactgcc gaccggtaaa gaatttatgc cgcgtgaaat cagtttgttg
    71221 attggtgaag agggaaatcc gctgagcagc caggaaatcc tggaaggcgg tgaatcgctg
    71281 atattatcgg aagtcgcaga gccgccagca caaatgattg attcactcac caccttattt
    71341 aaaaccatta agtcggtgaa gcgtgctttt atttgttcaa ttaaagagaa cgaagaggca
    71401 cagcctaatt tacttattgg cattgaagcc gatggtgata tcgaagaaat tattcaggcg
    71461 acgggaagtg tagcgaccga tacattacct ggcgatgaac caatcgatat ttgtcaggtg
    71521 aaaaaagggg aaaaaggaat tagccacttt attaccgaac atattgtgcc attctatgaa
    71581 cgtcgctggg gtggtttttt gcgtgacttt aaacagaatc ggataatcta aaatatgccg
    71641 ccccaaaggg cggcattcgg gcttacttgc tgctctgaag gttgttaacc accagtaaat
    71701 tgtcgccgga gagactccgg ttattattta cttgggaaaa cagcgcacca gaaacaataa
    71761 gtacgataca gaagcaccgt atcttcattg tgcggtccat gcgaagaccg catcacgaca
    71821 gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc taacggtcgc
    71881 ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg ccgcagtgga
    71941 taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga gttactggcg
    72001 agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg ctggaaaggc
    72061 cgcgaaccag accagcaata aaaaaccgcc aaattcggcg gttttttatt gcctgtaaaa
    72121 tacttatttc actggctcaa ccggtaaatc tgcccgcgcg ccccattcac tccatgcgcc
    72181 gtcgtacagt ttcacgtttg gcacatccag cgtcgcgagt gctaacaaaa ccacggctgc
    72241 cgttacacca gagccgcagc tgacgataat tggtttgtcg tagctgacgc cgcgaccaaa
    72301 aaatatcgca tccagttcat cggtcgtttt tagttcgcct tcgcgcacca gttccgtcca
    72361 cggaacattc agtgctccgg gaatatgtcc gcgacgtaaa cctgggcgag gttcatcaac
    72421 ttctgcgtta aaacgtgcag ccgggcgggc atcaataatt tgcgccgtat tttcatggct
    72481 tgccaataat acatcggtta ctttcaccac ggcttcagga ttaaacgcgg cgttaaactc
    72541 tccttccggc agctctactg caccttcttc taacagcaga tcatcgcgct gccagcctgc
    72601 aagtccaccc cccagaatcg acactttctc tacaccaaag gtgcgcagca tccaccatgc
    72661 tcgtggggct gagaaaagat taccttcgtc atagacaatc aggtgcttat cctggttaac
    72721 gcctaattca cgcatcgcca cggcgaacgt ttccgggcgc ggcagcatgt gcggaagcgg
    72781 ggaagtgtga tcagaaagcg cttcgatatc aaaaaacact gcgccgggaa tatgtccatt
    72841 cagatactcc tgagcaacgt tacgatcttc ctgtccaggc gacgccatgc gggcatcgat
    72901 aatctgaatt tccgggtcat caatatgttc ggcgagccag tcggctccta caaaccatgt
    72961 cgtggacata ggcatctcca tttttgccgg ggttaggcta aattgtcgga tgtttagtga
    73021 aaagcgacaa gcgaaagagg ctgatttggc tgtctgagcc acaattctca ctaaaattcg
    73081 gattgtatgg gcgtgatact gaattacata acgtaaattc tcacgcataa taattattcg
    73141 acgttgctaa cgggccattg tggcagggat gaaaaatgaa aaagttacgc gtagccgcct
    73201 gcatgctaat gctggcgctg gcagggtgcg acaacaacga taacgcgcca acagcggtga
    73261 aaaaagatgc gccttctgaa gttactaaag cggcctcttc agaaaacgcg agttcagcaa
    73321 aactctccgt gccggagaga caaaaactgg cccaacagag tgccggtaag gtgctgacat
    73381 tgctggatct ctctgaagtc caacttgatg gtgcagccac gctggtgctg acgttctcca
    73441 tccctctcga cccggatcag gatttctcac gcgttattca tgtcgtcgat aaaaaaagcg
    73501 gcaaagtgga tggtgcctgg gagctgtcag ataatcttaa agagttacgt ttacgccacc
    73561 tcgaaccgaa acgtgatttg atcgttacta ttggcaagga ggtcaaagca ctcaacaacg
    73621 caaccttcag taaagattac gaaaaaacta taactacccg cgacatccaa cccagcgtcg
    73681 gttttgccag ccgtggttcg ctgctgcctg gtaaagtcgt tgaagggctg ccggtaatgg
    73741 cgctcaacgt taataatgtc gatgttaact tcttccgcgt taagccagaa tctctgccag
    73801 cattcattag ccaatgggaa taccgcaatt cgctggcgaa ctggcagtca gacaaactgc
    73861 tgcagatggc ggatctggtc tacaccggac ggtttgatct caatcctgcg cgtaacaccc
    73921 gtgaaaaatt attgctgccg ctgggcgata tcaaaccgct tcagcaggcg ggcgtgtatc
    73981 tggctgtgat gaatcaggct ggacgttacg attacagtaa tcccgcgacg ctgtttacgt
    74041 taagtgatat cggcgtttca gctcaccgtt atcacaatcg tctggatatc tttacccaaa
    74101 gtctggaaaa cggcgcggcc cagcaaggaa ttgaagtctc tttattaaat gagaaagggc
    74161 agactctgac tcaggcaacc agtgacgctc aggggcatgt gcagctggaa aatgataaaa
    74221 acgcggcatt actgttggcg cgtaaagacg gtcagacaac gctactcgat ttaaaacttc
    74281 cggcgctgga cttagcagaa tttaacattg ctggcgcgcc aggctatagc aaacagtttt
    74341 tcatgtttgg cccacgcgat ctttatcgcc caggtgaaac ggtaatcctc aatggtttgc
    74401 tgcgtgatgc agacggtaaa gcgttgccca atcaacccat caagttagac gtgattaaac
    74461 ccgatgggca ggtactcagg agcgtcgtta gtcagccgga gaatggcctc taccacttta
    74521 cctggccact cgatagcaat gcggcaaccg gtatgtggca tattcgcgct aacacgggcg
    74581 ataaccagta tcggatgtgg gatttccacg tcgaagattt tatgccagag cgcatggcgc
    74641 tgaatctgac cggtgagaaa accccgctaa cgccgaaaga tgaagtgaaa ttctccgtgg
    74701 tggggtacta cctgtatggt gcacctgcta atggtaatac tttgcaaggg caacttttcc
    74761 tgcgcccact gcgtgaagct gtgtcagcct tacctggttt tgaattcggc gatatagctg
    74821 ccgaaaatct ttcccgcacg ctggatgaag ttcagttgac gctggatgat aaagggcgcg
    74881 gcgaagtttc tacagaaagc cagtggaagg aaacgcattc cccattacag gttattttcc
    74941 agggtagttt gctggaatcg ggcggtcgcc cggtgacgcg ccgcgctgag caggctatct
    75001 ggcctgccga tgcattgccg gggatccgtc cgcagttcgc ctcgaaatcg gtttacgatt
    75061 atcgcactga cagcacggtg aaacagccca ttgttgatga aggcagtaac gccggttttg
    75121 acatcgttta tagcgatgcg caaggcgtga aaaaagccgt gtcgggcttg caggtgcgcc
    75181 tgattcgcga acgccgcgat tactactgga actggtcaga agatgaaggc tggcagtcac
    75241 agtttgatca aaaagatctg atcgaaaatg aacaaactct ggatcttcag gcggatgaaa
    75301 ccggtaaggt cagtttcccg gtagagtggg gcgcttatcg tctggaagtc aaagcgccga
    75361 atgaagcggt cagtagcgtg cgtttctggg ctggctatag ctggcaggac aacagcgacg
    75421 ggagcggtgc cgtgcgaccc gaccgtgtca cgctgaaact ggataaagcc agttatcgcc
    75481 ctggcgatac cattaagttg catattgccg cgccaacggc gggtaaaggt tatgcgatgg
    75541 tcgagtccag tgaagggccg ttgtggtggc aagagattga tgttccggct caagggctgg
    75601 atctgacgat tccggtcgat aaaacctgga atcgtcatga tctgtattta agtacgctgg
    75661 tagtacgtcc tggcgataaa tctcgctccg cgacgccaaa acgcgcagtt ggggtgttgc
    75721 atctgccgct tggcgatgaa aaccgtcgcc tcgatctggc gctggaaaca ccagcaaaaa
    75781 tgcggccgaa tcaaccatta accgtgaaaa ttaaagccag cactaaaaat ggcgagaagc
    75841 ctaaacaggt gaatgtgctg gtgtctgccg ttgatagtgg tgtgctgaat attactgact
    75901 acgtcacgcc agatccgtgg caggcgttct ttggtcagaa acgctatggc gcagacattt
    75961 acgatattta cggtcaggtt attgaaggtc aggggcgtct ggcagctctg cgtttcggtg
    76021 gcgatggtga tgagctgaaa cgtggtggta aaccgccggt caatcacgtc aatattgtcg
    76081 cgcagcaggc gctgccggta acgctcaacg aacagggcga aggctcggtt acactgccga
    76141 ttggcgattt taacggtgaa ttgcgcgtca tggcgcaagc ctggacggca gatgatttcg
    76201 gtagcaacga aagcaaagtg atagttgccg caccggtgat tgctgaactg aacatgccgc
    76261 gctttatggc gagtggcgat acctcgcgtc tgacgctgga tatcactaat cttaccgata
    76321 aaccgcaaaa actgaacgtt gccctgaccg ccagtggttt gcttgaactg gtcagcgatt
    76381 cacccgcagc cgttgaatta gcgccaggtg tgcgtactac gctgtttatc ccggtgcgag
    76441 cattgccggg ttatggcgat ggagaaattc aggccaccat tagcgggtta gcgttaccgg
    76501 gtgaaaccgt tgccgatcag cataagcagt ggaaaatcgg cgtccgtccg gcgttcccgg
    76561 cacaaacggt taattacggt acggcgttac agcctggtga gacatgggcg attccggcgg
    76621 atggattgca aaacttctcg cctgttacgc tggaagggca attgttgttg agcggcaaac
    76681 caccgctgaa cattgcacgt tatatcaaag agttaaaagc gtatccgtac ggctgtcttg
    76741 agcaaaccgc cagcggcctg ttcccgtcac tttataccaa cgcagcccaa ctgcaggcgt
    76801 tgggcatcaa aggcgacagt gatgagaaac gccgtgcatc ggtcgatatc ggcatttccc
    76861 gtttgctgca aatgcaacgt gataacggcg gctttgcgct gtgggataaa aacggtgacg
    76921 aagagtactg gctgacggct tacgtgatgg atttcctggt ccgcgcaggc gaacagggtt
    76981 acagcgtgcc gacagacgcc attaaccggg gtaatgagcg tctgctgcgc tatttacaag
    77041 atccgggcat gatgtcgatc ccgtacgcgg ataatctcaa agccagtaaa ttcgccgttc
    77101 agtcttacgc tgcgctggtg ctggcccgtc agcaaaaagc accactgggt gcgctgcgtg
    77161 aaatctggga gcatcgtgca gatgccgctt ctggtttacc gctgctgcaa cttggcgttg
    77221 cgctgaaaac catgggtgat gcaacgcgtg gtgaagaagc gattgtgctg gcgctgaaaa
    77281 cgccgagaaa tagtgatgag cggatatggc tgggtgatta cggtagtcca ctgcgcgaca
    77341 acgcgttaat gctctccttg ctggaagaaa ataaactgct acccgatgag cagaacactt
    77401 tgctgaacac gctttcgcag caggcatttg gtgaacgctg gctatcgacg caggaaagta
    77461 acgcgttgtt cctggctgcc cgtacgattc aggatttacc gggtaaatgg caggcgcaaa
    77521 cctctttctc agctgagccg ctgacaggcg agaaaacgct aaacagcaat ctgaatagcg
    77581 atcaacttgc caccttgcag gtgagaaaca gtggcgatca gccgttatgg ttgcgtatgg
    77641 atgccagcgg ttatccgcaa tccgcacctt taccggcgaa caatgtgctg caaatcgagc
    77701 gtcatattct tggtactgat ggtaagagca aatcgctgga ctcgttacgt agcggcgatc
    77761 tggtgctggt ctggttgcag gtaaaagcca gtaacagcgt gccggatgcg ttagtcgtgg
    77821 atctgctgcc tgcgggtctg gaactggaaa atcagaatct ggcgaacggt agcgccagcc
    77881 tggagcaaag tggtggcgaa gtgcaaaact tactgaacca gatgcagcag gcgagcatta
    77941 agcacattga gttccgtgac gatcgctttg tggcggcggt tgccgttgat gaataccaac
    78001 cggtaacgct ggtgtatctg gcgcgggcgg tgacgccggg aacgtatcag gtaccgcaac
    78061 cgatggtgga atcaatgtat gttccccaat ggcgggcgac cggcgcggct gaagatctgc
    78121 tgattgtccg accgtaaatg cctcgcttgt taaccaaacg cggctgctgg ataacgctgg
    78181 cagctgcgcc atttctcctt tttcttgcag cgtggggagc agataaactc tggcctctac
    78241 cgctgcatga agtcaatccc gcacgagtgg tcgtggcgca ggatggtacg ccgctatggc
    78301 gcttcgccga tgctgacggc atctggcgtt atccggtaac aatcgaagat gtttctccac
    78361 gttaccttga agcgctgatc aattatgaag atcgctggtt ctggaagcat ccgggggtga
    78421 atccattctc ggtggcgcgc gcagcatggc aagatctcac ttcgggacgg gttatttccg
    78481 gtggcagcac gctcactatg caggttgctc gtctgcttga tcctcacccc aaaacatttg
    78541 gcggcaaaat tcgccagctc tggcgcgcgt tgcaactgga atggcatctg tctaagcgtg
    78601 aaattctgac cttgtatctt aaccgcgctc cgtttggcgg tacgttgcag gggatcggtg
    78661 cggcaagttg ggcttatctc ggaaagtcgc ctgcgaattt aagctattcc gaggcggcaa
    78721 tgctggcggt tttgccgcaa gcgcccagcc gtcttcgccc ggatcgttgg ccggagcgtg
    78781 ccgaagccgc gcgtaataaa gtgctcgaac ggatggccgt gcaaggtgcg tggtcccgtg
    78841 agcaggtaaa agagtcaagg gaagaaccca tctggctggc accccgacaa atgccgcaac
    78901 tggcaccgct gttttcgcgc atgatgctcg gtaaaagcaa aagcgacaaa atcgttacta
    78961 cgctggatgc cggtcttcaa cgacgtctgg aagaactggc gcaaaactgg aaaggtcggt
    79021 tgccaccgcg cagctcactg gcgatgatcg tggttgatca taccgatatg cgtgttcgcg
    79081 gctgggtggg atcggttgat ctcaacgatg attcacgctt tggtcatgtt gatatggtca
    79141 atgcgatccg atcgccagga tcggtgctca aaccgtttgt ttatggtctg gcgctggatg
    79201 aaggcttgat ccacccggca tcactgctgc aagacgtccc ccggcgcacc ggtgattatc
    79261 gaccaggtaa ctttgatagc ggttttcatg gcccgatcag catgagcgag gcgctggtgc
    79321 gctcgctgaa cttacctgct gtgcaggtgc tggaagccta tggaccgaaa cggtttgcgg
    79381 caaagttacg caatgttgga ttgccgttat atttgcccaa cggtgctgcg ccgaatcttt
    79441 cactcattct cggcggcgct ggtgcaaaac tggaagatat ggcggcagcg tataccgcgt
    79501 ttgctcgcca cggcaaggca ggcaaattgc gcttacagcc tgacgatccg ctgcttgaac
    79561 ggcctttaat gtcgtcgggg gcggcgtgga tcattcgccg gattatggct gatgaagcga
    79621 aacccttgcc ggatagtgcc ttgccgcgcg tcgccccact ggcatggaaa acgggcacca
    79681 gctatggcta tcgtgacgcc tgggcgattg gggttaacgc tcgctatgtc attgggatct
    79741 ggactggcag accggacggc acgcccgttg ttggtcagtt tggctttgcc agtgccgtac
    79801 cattgttgaa tcaggtcaat aatatcttac tgtcgcgcag tgcgaatctg ccggaagacc
    79861 cgcgcccgaa ctcagtcact cgtggcgtta tctgctggcc gggcggacag tctttgccgg
    79921 aaggtgacgg taactgtcgc cgccgcctgg caacctggct gctggacggg agtcagccgc
    79981 caactctatt actgccggag caggaaggca ttaatggcat tcgtttcccc atctggctgg
    80041 atgaaaatgg caaacgtgtt gccgctgatt gcccgcaagc gcgacaagaa atgataaatg
    80101 tctggccgct accgctggaa ccctggctgc ccgcatcaga acgtcgcgct gtgcgcttac
    80161 caccggcctc gacaatttgc ccgtcatacg gtcacgatgc ccagctccct ctgcagctga
    80221 caggcgttcg cgatggcgcg attattaaac gtttaccggg cgcagcggaa gcgactttgc
    80281 cgttgcaatc aagtggaggg gcaggtgaac gctggtggtt tctgaatggc gaaccgttaa
    80341 ctgaacgcgg gcgcaacgtg accctgcatt tgacggataa aggtgattat caattgctgg
    80401 tgatggatga tgtggggcaa atcgcgacag tgaaatttgt catgcaatag tctgttttgt
    80461 ccctgattgt tgctaaaact cattttattt taaaaaaatg ttaccttcat caatagtcaa
    80521 cggccctgtt gctcattata atccgcgcca tctcgtacgc tggtacagac aacaacagaa
    80581 caatttacag aggtaaaaat ggctattgaa cgtacttttt ccatcatcaa accgaacgcg
    80641 gtagcaaaaa acgtcattgg taatatcttt gcgcgctttg aagctgcagg gttcaaaatt
    80701 gtcggcacca aaatgctgca cctgaccgtt gaacaggcac gtggctttta tgctgaacac
    80761 gatggaaaac cgttctttga tggtctggtt gaattcatga cctctggccc gatcgtggtt
    80821 tccgtgctgg aaggtgaaaa cgccgttcag cgtcaccgcg atctgctggg cgcgaccaat
    80881 ccggcaaacg cactggctgg tactctgcgc gctgattacg ctgacagcct gaccgaaaac
    80941 ggtacccacg gttctgattc cgtcgaatct gccgctcgcg aaatcgctta tttctttggc
    81001 gaaggcgaag tgtgcccgcg cacccgttaa taatttcgta aatgccgcgt gcaaacgtgg
    81061 catccgtgcg ccagaatttg tacaatgcag cgcccccgga cgagcagccg ctcaccgggg
    81121 cgtttctttt ttcaatcctc caggggccat aacgtgtaat aacgaggccg gaatagcata
    81181 tgtctgaaca attagtcaca cctgaaaacg tcaccacgaa agatggaaaa atcaacctgc
    81241 tggatctcaa ccgtcagcag atgcgggagt tttttaaaga tttaggtgaa aaacccttcc
    81301 gcgccgatca ggtgatgaag tggatgtatc actattgctg cgacaacttt gatgagatga
    81361 ccgacatcaa caaagtgttg cgcggcaaac tgaaagaggt ggcggaaatc cgcgcaccgg
    81421 aagtggttga agaacagcgt tcatccgacg gcaccattaa atgggcgatc gccgttggcg
    81481 atcagcgcgt cgaaacggtg tatatcccgg aagacgaccg tgccacgctc tgcgtctctt
    81541 cgcaggtggg gtgtgcgctg gagtgtaaat tctgttccac cgcccagcag ggctttaacc
    81601 gcaacctgcg ggtatcggaa attattggcc aggtgtggcg tgcggcgaaa atcgtcggcg
    81661 cagcaaaagt caccggtcag cgcccaatca ctaacgtagt gatgatgggc atgggcgagc
    81721 cgctgctcaa cctgaacaac gtcgttccgg cgatggaaat catgcttgat gatttcggtt
    81781 ttggcctgtc taaacgtcgc gtcacgcttt ctacttccgg tgtagttccg gcgctggata
    81841 aactgggcga tatgatcgac gttgcactgg caatttccct gcacgcgccg aacgacgaaa
    81901 ttcgtgacga aattgtgccg atcaacaaaa agtacaatat cgaaacgttc cttgctgcgg
    81961 tgcgccgtta tctggagaaa tccaacgcca atcagggccg tgtcactatt gagtatgtga
    82021 tgcttgacca cgttaacgac ggcactgaac acgcgcacca actggcggaa ctgctgaaag
    82081 atacgccgtg taagatcaac ctgatcccgt ggaacccctt cccgggcgcg ccgtatggac
    82141 gcagctcgaa cagccgtatc gaccgtttct caaaagtgct gatgagctac ggtttcacca
    82201 ctattgtgcg taaaacccgt ggtgatgata tcgatgctgc ctgtggtcag ttggcgggcg
    82261 atgttatcga ccgtacgaaa cgtaccctgc gtaaacggat gcagggtgaa gccatcgaca
    82321 ttaaagcggt ctgatagcca ttacgccacg gttactttat gtaccgtggc gtaatctttt
    82381 atgaatgaag tattaagttg caattgtctg gtcaatatta acggtgcgtt tttgctgact
    82441 ttaaggcagt atgtaacgat gcaacagtaa gttagcctta gtgaatgtgg gctttgtcac
    82501 gagcacacag acggtcttat actgtatgat aacggttaac ttaacggatg tttcgcggtg
    82561 tgggtgagca tgatggttca ccggcatctc aattctcatt taaacgtacc tgcagcgaat
    82621 gaatactgaa gccacgcacg accaaaatga agcacttact accggcgctc gcctgcgtaa
    82681 tgctcgcgaa caactaggac ttagtcagca ggccgttgcc gagcgacttt gcctgaaggt
    82741 ttccacggta cgcgacattg aagaagataa ggcacccgcc gatcttgctt caacattcct
    82801 gcgcggatat atccgctctt atgcgcgtct ggtacatatt ccagaagaag aactgctgcc
    82861 agggctggaa aagcaggctc cactacgggc tgcaaaagtt gcgcctatgc agagtttttc
    82921 cctcggtaaa cgccgcaaaa aacgtgacgg ctggctgatg accttcacct ggctggtgtt
    82981 gtttgtggtt atcggcctga gcggtgcctg gtggtggcaa gaccacaaag ctcagcagga
    83041 agagatcacc actatggccg atcaatcttc ggcggaactg agcagtaata gcgagcaggg
    83101 gcagagtgtt ccgttaaata cgtcgacaac tacagacccg gctacaacgt ccacgccgcc
    83161 agcgtctgtg gatactaccg caaccaacac gcaaacacct gccgtaactg cgccagcacc
    83221 agctgttgat ccgcaacaga atgcggttgt ttcgccttcg caggcaaatg ttgataccgc
    83281 cgcgaccccg gcaccaacgg caacaacaac gccagatggc gctgcgccgt tgccaaccga
    83341 tcaggcaggc gtgactacgc cggcggctga tccgaatgcg ctggtgatga actttactgc
    83401 cgattgctgg ctggaggtta ctgatgctac cggtaaaaaa ttgtttagcg gtatgcagcg
    83461 taaagacggt aatttgaact taaccggcca ggcaccatac aaactgaaaa ttggtgcgcc
    83521 agccgcagta cagatccagt atcaagggaa acctgtcgat ctgagtcgtt ttatcagaac
    83581 taaccaggtt gcgcgtctga ccctcaatgc cgaacaatca ccggcgcagt aacagacggg
    83641 taacgcggga gatttttcat gcataaccag gctccaattc aacgtagaaa atcaacacgt
    83701 atttacgttg ggaatgtgcc gattggcgat ggtgctccca tcgccgtaca gtccatgacc
    83761 aatacgcgta cgacagacgt cgaagcaacg gtcaatcaaa tcaaggcgct ggaacgcgtt
    83821 ggcgctgata tcgtccgtgt ctccgtaccg acgatggacg cggcagaagc gttcaaactc
    83881 atcaaacagc aggttaacgt gccgctggtg gctgacatcc acttcgacta tcgcattgca
    83941 ctgaaagtag cggaatacgg cgtcgattgt ctgcgtatta accctggcaa tatcggtaat
    84001 gaagagcgta ttcgcatggt ggttgactgt gcgcgcgata aaaacattcc gatccgtatt
    84061 ggcgttaacg ccggatcgct ggaaaaagat ctgcaagaaa agtatggcga accgacgccg
    84121 caggcgttgc tggaatccgc catgcgccat gttgatcatc tcgatcgcct gaacttcgat
    84181 cagttcaaag tcagcgtgaa agcgtctgac gtcttcctcg ctgttgagtc ttatcgtttg
    84241 ctggcaaaac agatcgatca gccgctgcat ctggggatca ccgaagccgg tggtgcacgc
    84301 agcggggcag taaaatccgc cattggttta ggtctgctgc tgtctgaagg catcggcgac
    84361 acgctgcgcg tatcactggc ggccgatccg gtcgaagaga tcaaagtcgg tttcgatatt
    84421 ttgaaatcgc tgcgtatccg ttcgcgcggg atcaacttca tcgcctgccc gacctgttcg
    84481 cgtcaggaat ttgatgttat cggtacggtt aacgcgctgg agcaacgcct ggaagatatc
    84541 atcactccga tggacgtttc gattatcggc tgcgtggtga atggcccagg tgaggcgctg
    84601 gtttctacac tcggcgtcac cggcggcaac aagaaaagcg gcctctatga agatggcgtg
    84661 cgcaaagacc gtctggacaa caacgatatg atcgaccagc tggaagcacg cattcgtgcg
    84721 aaagccagtc agctggacga agcgcgtcga attgacgttc agcaggttga aaaataataa
    84781 cgtgatggga agcgcctcgc ttcccgtgta tgattgaacc cgcatagctc ccgaaacatt
    84841 gagggaagcg ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca
    84901 ttcaagccat tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca
    84961 ttgaaggcac actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga
    85021 ttgtagagca gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa
    85081 aagagatgta cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga
    85141 cggcgggctg tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc
    85201 tgtggtatat cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt
    85261 tccatcagtt gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga
    85321 ttatgctcac cgcccgctgg tggcgtgcgc tgggtatttc cgagcacgta actcttgagc
    85381 tgaactctat cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc
    85441 ttgagcagta taaagataag ctggacgaag actgcaaacg ccgcatgtac actaacccgc
    85501 tgcgcgtgct ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat
    85561 taggtgatta tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgcttg
    85621 agagcgcggg gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata
    85681 accgtaccgt tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag
    85741 gcggtcgtta tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt
    85801 ttgcgatggg cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag
    85861 ccgatcctgt tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta
    85921 tggcattagc tgagcgtctg cgtgatgaat taccgggcgt gaaattgatg accaaccacg
    85981 gcggcggcaa ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg
    86041 tggtgctggg tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg
    86101 gtgagcaaac ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt
    86161 aaggaaggag aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt
    86221 taaacgcttt tttgctgaaa atggcaaagc actggctgtt ggggtgattt tgggcgttgg
    86281 cgcactgatt ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc
    86341 tcttgcctat caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc
    86401 ggcggaaaaa tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact
    86461 tgcgcagcaa tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg
    86521 gctggcagac acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt
    86581 tcaggtacag ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga
    86641 agggtgggct gccattgttg ccgacctgcg tggtgaagca ttgctgagca aaggtgataa
    86701 gcaaggtgcg cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag
    86761 cgaaatgatg cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc
    86821 gtaaattact gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta
    86881 acagcgaaga agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc
    86941 cgaccacggc gtggagtact tccgttggta gcggcattgg caacttctat tccaatcttc
    87001 atccggcact ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc
    87061 tgaatgcgga tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt
    87121 tctctaaaga gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca
    87181 ttggcagcga aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc
    87241 aaactaaagt cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa
    87301 tccacaccag taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga
    87361 cagttaacct cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt
    87421 ttggtgcggc cgtcgtgggg ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg
    87481 gccagatgat ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc
    87541 tgagcgatgt tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata
    87601 atggtaacct gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg
    87661 gttcggtgaa tgatttcatc gtcgacggca atcgcatcta tctggtcgat caaaatgacc
    87721 gggtgatggc gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc
    87781 atcgcctgct gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag
    87841 gttatctgca ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata
    87901 gttccggttt ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag
    87961 acggaaccgt gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc
    88021 ctggacaggg gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga
    88081 tgatttataa aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta
    88141 acgtaggaaa atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg
    88201 atttcccggg tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt
    88261 ttatctgtat tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg
    88321 cggaacagtc gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc
    88381 gcgcgggcct gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac
    88441 cgaccttcct ggtggcaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt
    88501 tctactcgct tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat
    88561 taagtctgct ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag
    88621 tcgacgaaga cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag
    88681 aggaagacga cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga
    88741 acgtaggtaa gtctacactc actaaccgta ttcttggtga agagcgcgtt gttgtttatg
    88801 acatgcctgg cacgacgcgt gacagtatct acattccaat ggaacgcgat ggacgtgagt
    88861 atgtgctcat tgataccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga
    88921 aattctccgt aatcaaaacg ttgcaggcca ttgaagatgc caacgtggtg atgttagtga
    88981 ttgatgcgcg cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata
    89041 gtgggcgctc acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag
    89101 agcaggtgaa agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact
    89161 ttatctctgc cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt
    89221 atgacagctc cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg
    89281 ttgaagatca ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg
    89341 ctggtggtta taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg
    89401 attcctacaa gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc
    89461 cgattcgtat tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga
    89521 cgccaaccca gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat
    89581 tatccgtaag tattttatct gccattccct ctatgaatgt agagggatgg ctaccggagg
    89641 aattcatatg gaactgcatt gtccacaatg tcagcatgtg cttgatcagg ataacggcca
    89701 tgcgcgttgc ccatcctgcg gggaaattat tgaaatgaaa gctctttgtc cagactgcca
    89761 tcaaccgttg caggtgttaa aagcctgcgg tgcagtagat tacttctgtc agcacgggca
    89821 tggcttgata tcgaaaaaac gcgttgaatt cgtgctggct taatgcacct ttttacgcga
    89881 tttttttact ggctggatgt ttttaacttc actttctatc cagccgtctt ccagacgtgt
    89941 ggttagcatt tcacccgctt taacttgctt cacttttttc agtacattgc cgtcagtagc
    90001 agtagtaacg ctgtatccac gcgccagcgt tgacagtggg cttacggctt cgaggtgcgt
    90061 tactgcatta ccgaaacgtt cacgcgtggc gctaagctgt acgcgcaggg tttctgctaa
    90121 acgatattcc agttgctgaa tgcgcgtttg cgcgcgatga atcttcggct gtggattttg
    90181 ctgattcagc cgctgtgtta accgctgctg ctgttgcccg gtacgcttaa gttgattttc
    90241 cagcgcaaag ctcatccgct tttgcaggcg ctcaagcatg gtttgctggc gtgccagccg
    90301 gagctgcgga tgctgttgct gtaatcggtg atggatctgc gtaaagcgac gcgtgcggtt
    90361 ggcgagataa taatccatcg ccatctccag ccgttgacgg gtcgattgca cctggcgcag
    90421 taactcttgc tgattacggc tcactacttc agcggcggca gacggcgttg gcgcacgcag
    90481 atcggcaaca aaatcggcaa tggtcacatc cgtctcatgc ccgacggcgc tgacgaccgg
    90541 aatgcggctg gcaaaaatcg cccgcgctac gcgttcgtcg ttaaaactcc ataaatcttc
    90601 cagcgaaccg ccgccgcgcc caacgatcaa cacgtcgcac tcattgcgct gattcgccag
    90661 ttcaatggcg cgaacaattt gccccggtgc gtcatcgccc tgaacggcgg tggggtagat
    90721 gatcaccggt agagaaggat cgcgacgttt taacacatgc aaaatatcat gtagcgcagc
    90781 accggttttt gaggtgatca caccaacgca atgcgcaggg gagggaagtg gttttttgta
    90841 ttgctgatcg aacaaacctt cagcctgcaa cttcgctttg agctgttcgt acttctgttg
    90901 cagcagccct tcaccggccg gctgcatact ctcaacgatt atctggtagt caccgcgcgg
    90961 ctcgtagagc gtaatattgg cgcgaactaa aacttgttgt ccatgctgtg gacggaaggt
    91021 cacccggcgg ttgctgttgc ggaacatcgc gcagcgtacc tgggcggtgt cgtctttgag
    91081 tgtaaagtac cagtgaccgg aagccggttg tgtgaaatta gagatttcgc cgctgatcca
    91141 aacctgtccc atctcatgct caagcagcag acgaaccgtt tgattcaggc gactaacggt
    91201 aaaaattgca ggggattgag aaggtaacat gtgagcgaga tcaaattcta aatcagcagg
    91261 ttattcagtc gatagtaacc cgcccttcgg ggatagcaag cattttttgc aaaaaggggt
    91321 agatgcaatc ggttacgctc tgtataatgc cgcggcaata tttattaacc actctggtcg
    91381 agatattgcc catgctacgt atcgctaaag aagctctgac gtttgacgac gttctcctcg
    91441 ttcctgctca ttctaccgtt ctgccgaata ctgctgacct cagcacccag ctgacgaaaa
    91501 ctattcgtct gaatatccct atgctttccg cagcaatgga taccgtaacg gaagcgcgcc
    91561 tggctattgc tctggctcag gaaggcggta tcggctttat ccacaaaaac atgtccattg
    91621 aacgccaggc agaagaagtt cgccgtgtga aaaaacacga atctggtgtg gtgactgatc
    91681 cgcagactgt tctgccaacc acgacgctgc gcgaagtgaa agaactgact gagcgtaacg
    91741 gttttgcggg ctatccggtc gttaccgaag aaaacgaact ggtgggtatt atcaccggtc
    91801 gtgacgtgcg ttttgttacc gacctgaacc agccggttag cgtttacatg acgccgaaag
    91861 agcgtctggt caccgtgcgt gaaggtgaag cccgtgaagt ggtgctggca aaaatgcacg
    91921 aaaaacgcgt tgaaaaagcg ctggtggttg atgacgaatt ccacctgatc ggcatgatca
    91981 ccgtgaaaga cttccagaaa gcggaacgta aaccgaacgc ctgtaaagac gagcaaggcc
    92041 gtctgcgtgt tggtgctgca gttggcgcag gtgcgggtaa cgaagagcgt gttgacgcgc
    92101 tggttgccgc tggcgttgac gttctgctga ttgactcctc ccacggtcac tcagaaggcg
    92161 ttttgcagcg tatccgtgaa acccgtgcta aatatccgga tctgcaaatc atcggcggca
    92221 acgtggcaac agctgcaggt gcacgcgctt tggcagaagc tggttgcagc gcggttaaag
    92281 tcggtatcgg ccctggttct atctgtacga ctcgtatcgt tactggcgta ggtgttccgc
    92341 agatcactgc cgttgctgac gcagttgaag ccctggaagg caccggtatt ccggttatcg
    92401 cagatggtgg tattcgcttc tccggcgaca tcgccaaagc tatcgccgct ggcgcaagcg
    92461 cggtgatggt aggttccatg ctggcgggta ctgaagaatc tccgggtgaa atcgaactct
    92521 accagggccg ttcttacaaa tcttaccgtg gtatgggttc cctgggcgcg atgtccaaag
    92581 gttcctctga ccgttatttc cagagcgata acgctgccga caaactggtg ccggaaggta
    92641 tcgaaggtcg cgtagcttat aaaggtcgcc tgaaagagat cattcaccag cagatgggcg
    92701 gcctgcgctc ctgtatgggt ctgaccggct gtggtactat cgacgaactg cgtactaaag
    92761 cggagtttgt acgtatcagc ggtgcgggca ttcaggaaag ccacgttcac gacgtgacca
    92821 ttactaaaga gtccccgaac taccgtctgg gctcctgatt ctcttcgccc gacttcatgt
    92881 cgggcgattt atattatctg tttcacttgc ctcggaataa gcgtcaatga cggaaaacat
    92941 tcataagcat cgcatcctca ttctggactt cggttctcag tacactcaac tggttgcgcg
    93001 ccgcgtgcgt gagctgggtg tttactgcga actgtgggcg tgggatgtga cagaagcaca
    93061 aattcgtgac ttcaatccaa gcggcattat tctttccggc ggcccggaaa gcaccaccga
    93121 agaaaacagc ccgcgtgcgc cgcagtatgt ctttgaagca ggcgtaccgg tattcggcgt
    93181 ttgctatggc atgcagacca tggcaatgca gttgggcggt cacgttgaag cctctaacga
    93241 acgtgaattt ggctacgcgc aggttgaagt cgtaaacgac agcgcactgg ttcgcggtat
    93301 cgaagatgcg ctgaccgcag acggtaaacc gctgctcgat gtctggatga gccacggcga
    93361 taaagttacc gctatcccgt ccgacttcgt caccgtagcc agcaccgaaa gctgcccgtt
    93421 tgccattatg gctaacgaag aaaaacgctt ctatggcgta cagttccacc cggaagtgac
    93481 tcacacccgc cagggtatgc gcatgctgga gcgttttgtg cgtgatatct gccagtgtga
    93541 agccctgtgg acgccagcga aaattatcga cgatgctgta gctcgcatcc gcgagcaggt
    93601 aggcgacgat aaagtcatcc tcggcctctc tggtggtgtg gattcctccg taaccgcaat
    93661 gctgctgcac cgcgctatcg gtaaaaacct gacttgcgta ttcgtcgaca acggcctgct
    93721 gcgtctcaac gaagcagagc aggttctgga tatgtttggc gatcactttg gtctgaacat
    93781 tgttcacgta ccggcagaag atcgcttcct gtcagcgctg gctggcgaaa acgatccgga
    93841 agcaaaacgt aaaatcatcg ggcgcgtttt cgttgaagtg ttcgatgaag aagcgctgaa
    93901 actggaagac gtgaagtggc tggcgcaggg caccatctac cctgacgtta tcgaatctgc
    93961 ggcttctgca accggtaaag cacacgtcat caaatctcac cacaacgtgg gcggcctgcc
    94021 gaaagagatg aagatgggcc tggttgaacc gctgaaagag ctgttcaaag acgaagtgcg
    94081 taagattggt ctggagctgg gcctgccgta cgacatgctg taccgtcacc cgttcccggg
    94141 accaggcctt ggcgttcgtg tgctgggtga agtgaagaaa gagtactgtg acctgctgcg
    94201 ccgtgctgac gccatcttca ttgaagaact gcgtaaagcg gacctgtacg acaaagtcag
    94261 ccaggcgttc actgtgttcc tgccggtacg ttccgttggc gtaatgggcg atggtcgtaa
    94321 gtatgactgg gttgtctctc tgcgtgctgt cgaaaccatc gactttatga ccgcacactg
    94381 ggcgcatctg ccgtacgatt tcctcggtcg tgtttccaac cgcattatca atgaagtgaa
    94441 cggtatttct cgcgtggtgt atgacatcag cggcaagccg ccagctacca ttgagtggga
    94501 atgatttgac cctgcactat gaatgaacaa aaccctctgt tactacagag ggttttttat
    94561 cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc tttccgattt
    94621 gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag tcatatttct
    94681 gttggcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct tcagttcctg
    94741 atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg ccagtttttg
    94801 tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt ttttgtccgc
    94861 agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg ccgttgccgc
    94921 tcttttgtta gcggcggcct gggcatttgc gcgacgttgc ttctcttgct ggatttccct
    94981 gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac cttgctgttc
    95041 cgcttctgcg acagcagaga gttgatcctg caatgatgag gcgatagccg gatagcttaa
    95101 ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt attagctctt
    95161 ttcaggacat ttagtatttg gctgaatacg cgtttcgtta tacgttgtgg taataacaac
    95221 ggctaaacct gtcgtaaact ggcactcttt acccacctgg gtagaggtat acactttggt
    95281 gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca ccatagaacc
    95341 cactgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg aattgctgcc
    95401 agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg cgccgaaggc
    95461 ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa gaatggaaat
    95521 aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat aaacatcggc
    95581 ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta acattgccac
    95641 aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt acgaaaaata
    95701 agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg gatagattaa
    95761 atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa catatatatt
    95821 aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa agacgtaaaa
    95881 tttaagatga aagaatatct gccgctttga taattccaaa gagcaaaagc atccgacagc
    95941 gaagtgccgt taggttctgt gaaagtggag aaggtaaccg ctaacgttgt accggtgatt
    96001 cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc caggcaccga
    96061 cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt acgatacggg
    96121 tcatgcgact gttaaatcgg tgtcgttttc gcatactggt tgcctgactc acaaaaggtt
    96181 ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca ggacaaaatg
    96241 gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt tatggaagtt
    96301 aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa acgtaataat
    96361 tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata tgacacttag
    96421 aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg atgcctgtct
    96481 gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac tgaatgcaac
    96541 ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt ctttaatctt
    96601 gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg tttttctctt
    96661 ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg cggcattgcc
    96721 agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt acgaaacgct
    96781 ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt acagggtctt
    96841 tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc agcgtatatt
    96901 ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg ctgcatttgt
    96961 aggattgctg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc tcgggacttt
    97021 aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt gctacttcat
    97081 cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat taaaacagca
    97141 ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat taggtgcttt
    97201 attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca ccaattatac
    97261 cttgtcatta ttgctgcccc tgatgatgtg gggagcgatg cgctatggtt ataagctgat
    97321 ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa attacattcc
    97381 catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc tggtattctc
    97441 ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag tcagacgcat
    97501 tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg ccctgaatcg
    97561 cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg gcatggaaat
    97621 gctggttaag aactatggca tcatgctgcg gattcaatac aagcaaaaac tttctcactg
    97681 gctgtcaccc ttgctggaac cgggtgaaga tgtttatcag ctttcgggta acgatctcgc
    97741 gctgcggctg aatacagaat cgcaccagga gcgcattacc gcactggata gccatctcaa
    97801 gcaatttcgt ttcttttggg atggcatgcc gatgcaaccg cagattggcg tcagttactg
    97861 ctatgtgcgc tcgccagtga atcatatcta cctgctgctg ggagagctaa atacggtcgc
    97921 cgaactttcc atcgtgacca acgccccgga aaatatgcag cgtcgcgggg caatgtattt
    97981 gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcga ctacagcagg cgctggaaca
    98041 caaccatttt ttcctgatgg cccagccgat taccggtatg cgtggtgatg tttaccatga
    98101 aattcttctg cgcatgaaag gtgagaatga tgaactgatc ggccccgata gcttcttgcc
    98161 ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg tgggtcattg agcatacgct
    98221 gcaatttatg gctgaaaaca gagcgaagat gcccgctcac cgttttgcta ttaatctgtc
    98281 tccaacctcg gtatgtcagg ctcgttttcc tgttgaagtc agtcagttgc tggataaata
    98341 tcagattgaa gcgtggcaac ttatttttga agtcaccgaa agtaatgctc tgaccaatgt
    98401 taagcaggcg caaatcacct tgcagcatct tcaggaatta ggctgccaga ttgcgattga
    98461 tgatttcggc accggctacg ccagctatgc gcggcttaaa aatgtgaatg ccgatctgct
    98521 taaaattgac ggcagtttta tccgcaatat tgtgtcaaat agtctggatt atcagatagt
    98581 ggcatcgatt tgccacctgg cgcgaatgaa gaaaatgcgg gtagtggcag agtacgttga
    98641 aaacgaagag atccgcgagg cggtgctctc tttggggatc gattatatgc agggttatct
    98701 tattggtaag ccgcaaccgt taattgatac gctgaatgaa atcgaaccca ttcgcgaaag
    98761 tgcctgaata atgcgggccg acatttctcg tcggcccgca aagcattaag cggcgatttc
    98821 aggtgtactt tcttcttcaa ttttcaaccg ccagccagcc acgccttccc agtattcttg
    98881 ctccttttcc agatcaagca gtaccagcgc attctgacta aaccagtcat gcgggaaacg
    98941 cagtgtccag tgactgtcat cagtaatcag tgtcaatgtt ggcggtgtgg tggttgcctg
    99001 acgttgattg tttagtaata cgccaaggcg caatagctgt atcagtggca ggaactgttt
    99061 cttcttaaac aaggtaaagc gcggcagatc gtcgagctta atcgctttac ggtgatagcg
    99121 caccagtgtc gccatcatca gctgctgttc ctgattaaaa cccggcaagt cactgttttg
    99181 cagaatataa gcggagtggc gatgcaaacc gctgtggttg atattcaacc cgacctcatg
    99241 cagcatggcg gcccatcgca gtagcgcctc cagttgcgga tgcgccagct tcggttgctg
    99301 ttcccgccac tgttcgtaca tttgcatagt ggtatccagc actcgtcggg cctgttcgct
    99361 gtcgatgtga tactggttgg cgaggctgct ggcggtacga ctacgcacat cctgatgacg
    99421 gaaacgtcct tccatttcat acagtacgcc ttcacgtaaa gccccgtcag aaaggcgcag
    99481 ttcacggatg gctaaagcat caaacacacc gcataaaatc gccagtccag gaacgaagac
    99541 tgttttccgc tcttcggaaa gacccggtaa actcagcgat gcgaaattac ggtgacgtaa
    99601 aacttctttt accagttttt ccagacgttc cggggtaatt atcccgtctt tctcgcccat
    99661 ttccattaat acttcatggg cggcttttat ggtaccggaa gcgcccattg ccacgttcca
    99721 gccctgaata cggaattgcc aggttaaagt ttccagtttt tgtgctgccg ccatgcgagc
    99781 gcgctggaaa ttctctttat tgatgacccc gccagggaaa taaagctggg caaagctgac
    99841 acaacccatc cggcggcttt caacgagaat aggttcgaaa ttttcaccaa tcaccagttc
    99901 cgtagatccg ccgccaatat caataaccag tttgcgacct ttttccggtt gggtatgttc
    99961 cactcccata aaaatcagac gggcttcttc attaccggaa ataatttcaa tcgggtaggg
   100021 aatgaccttt tccgcgcgtt tcagaaagtc agtggcgttc agcgcctgac gcagcgtatg
   100081 ggtaccaact atacagacgc tggcaggaga aaacccttgt agccgttcgg caaaaagcga
   100141 cagacagttc aaaccgcgcg tcattgcctc ttcactcaac atattatctg gccccaggcc
   100201 gtccgccaga tgcacccgct gtttcaggcg gccaataatc tgcatggcac catctaccac
   100261 acgggctatg accatgtgaa aactgtttga accaagatcg accgcagcaa actcctgcgg
   100321 acgaggggat ttatcgtgta ttggcatagg gttattcagg ttgttcgagt gatttgatgt
   100381 agtcataaat cgccaactgc gcccgtactt tgcggcgatt gccgcgggga acgtagcgat
   100441 tactgagttc tttatcgata taacgagctt tgaccgtatc gctgaacaat atgtcgatga
   100501 tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg cgtcgccact tcaatacgat
   100561 aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta gaccttttta tcgccgccat
   100621 tttcaaaaat ataaacccgg tcatgttcaa ggtaacggtc aacaatactg atggcacgaa
   100681 tgttgtcgct aatgccttcc agattgggga tcagcgaaca cattccgcga accagcagat
   100741 taaccggtac gccggagctg gaggccgcat acagacgatc aaccaggcct ttatcgacaa
   100801 ggttatttag cttcagggtg ataccgctgg gcagcccttg ctgcgcgttg gcgatttcgc
   100861 ggtctaccat ttcatataac agacggcgag agttttgcgg tgacaccatt aaataatcaa
   100921 atgtcaccgg gcggtatggg ttttcaataa agttaaatac ccgccgtact tcgttggtga
   100981 tacgcgcatc ggcggtcagc aatgaatagt cagtataaag acgcgcggtt ttttcgttaa
   101041 agttcccggt cccgatgtgt gcgtaacgca ccacttcacc gttttctttt cgtgaaatca
   101101 ggaacagttt ggcgtgaatt ttcagccccg gcgcagagaa gataacgtgc acgcctgctt
   101161 cggtcaggcg cttcgcccag tgaatgttag cttcttcgtc gaaacgcgcc tgtaactcaa
   101221 ccaccacggt gactttctta ccgttatgtg cggcgtggat catcgagtcg atgatgcgtg
   101281 aatctttcgc cacgcggtaa atgttaattt taatcgccag tacgctcggg tcgaacgaag
   101341 cctgacgcag cagttccagc acatgctcaa aggtgtgata aggataatag agcaacacat
   101401 cgcgttcgcg aatggcatca aaaccattgc ggaactgggc tttatcaaac caaatatggc
   101461 gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc gacattgggg aaattaataa
   101521 agtctttaaa attatgataa cgaccgccgg ggacgatgga gtcgtagcgg gaaatagtca
   101581 gtttttcgcg taacacttca accagcgcat tgggcatatc gcgctgataa acaaaacgca
   101641 ccggctcagc agttaaacgc tgcttgagac tggaagacat caactccatc aggctggctt
   101701 ccatctcatg cactaaatcg tattcggcat cgcgggtcat cttcattgaa taggcattca
   101761 gcgcgtcata atcaaagaag cctttgaaaa tatcatcaag gcagtaacgc agaatgttat
   101821 ccagaagaat catcggcttg cgtcgacgcg gcgcttctgg cggtaaattc acaaagcgcg
   101881 gcactttatc tgatgggatc tccagcagcg cgtaacggat ggtatcgcca cggataattt
   101941 ccaccgccag ataggtgtaa tcatctttca ggaactgcac taagtcagtg tcaggattga
   102001 ttaaaatcgg cgtaatgtgc tgacgcagat actgcttaaa ataatgacgc agccagtttt
   102061 gttgattgac ggagagctgg cgttcattaa tcaggaagat ctggttacgc gccatctcca
   102121 gcagcagctc gttgtagagg ccgtcgaatt cctgatcggc tttcagcacc cgggactgaa
   102181 ttttgcccag taaatggcgg gaatgagagt tggagccttg ttcttcgcta ataatgatgc
   102241 gtcgcttcag ttcagcgaag cggactttat agaactcatc aaggttattg gaatagatcc
   102301 ccaggaaacg catcctttca atcagcgggt tagatttgtc cgccgcttcc tgaagcacgc
   102361 gttcattgaa cgataaccaa ctgagctctt tttcgatgta tagcttttcc tgacccatta
   102421 ccacttttac tccgttttat tcacgggaca ctgcctggat attatggcga gcaatattgg
   102481 cagatgtcca actatgccag aaaaggggga aaaaaactgc tggagcttgc gatttacagg
   102541 tgaattgaaa aacgccagcg gcagagctgg cgctttaact acggggcatt actcgtcggc
   102601 agcgtagccc tgcggcggca gacgttgacc atccagccac gcggcgtttt cgtgcatttt
   102661 cagacgacca tcggcaaacc agctaatcac cagtggataa atagcgtgtt cctgggtttg
   102721 cacgcgggcg gtgatgtcat cttccgtatc accagcaaat accgggactt tcgcctgtaa
   102781 aataaccggg ccaccgtcca gttcatcggt gacgaaatgc accgatgtac cgtgctcttc
   102841 atcaccattt tccagcgcct gacgatgggt gtgtaatccg ggatatttcg gcagcagaga
   102901 agggtgaatg ttcagcaaac gcccggcata gtgggagaca aacgccgggc tgagaatgcg
   102961 cataaaacca gccagcacga ccacatcggg tgcgtacatg tcgatttcat gaatcaactc
   103021 ccggtcatag gcctcacgac tgtcaaacgc gctggcgatg agcgtatgcg ttgcaatacc
   103081 cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta ttgctgaaaa ctgcccgtac
   103141 ggtgccttta attttgttgg ttttacaggc gtcaataatt gcctgtaaat tacttccgtt
   103201 gccggaaata agcaccacaa tattcattat tcgataacca cgcgttgttc ggaatcagag
   103261 gctttgatga taccgatttt ccacgcgttt tcaccgttgg cattgagtag ggcgagggct
   103321 ttgtccactt ccggagcagg cagggcaata atcatcccga cgccgcagtt gaaggtgcga
   103381 tacatttcat ggcgctcaac gttacctgcc gtttgcagcc agttgaacac ttccggccac
   103441 tgccaggaag attcatcaat cactgcctgg gtattatctg gcaatacgcg cggaatgttt
   103501 tcccagaagc cgccgccggt caggtgcgca atggcatgca catcgacctt ttcaatcaac
   103561 tccagcactg acttcacgta aatgcgggtc ggtgccagca gatgatcggc taatggctta
   103621 ccatcaagtt cggtggtttg cggatcacaa ccgctgactt caagaatttt gcgcaccagc
   103681 gaatagccgt tcgagtgcgg accgctggaa ccgagtgcaa tcagcacatc gccgtcgctg
   103741 actttagagc cgtcgatgat ttctgatttt tctaccacgc caacgcagaa acccgcgaca
   103801 tcgtaatctt caccgtgata catccccggc atttctgccg tttcgccacc caccagtgaa
   103861 cagcccgatt gcagacatcc ttccgcaatg ccgctgatca ccgctgaagc ggtatcaaca
   103921 tccagttttc cggttgcgta atagtcgagg aaaaacagcg gttctgcacc ttgcaccacc
   103981 aggtcattaa cgcacatggc gaccagatca ataccaatgg tgtcgtgacg ttttaagtcc
   104041 attgccagac gcagcttggt acctacgccg tcagtgccgg aaaccagcac gggttcacga
   104101 tatttttgcg gcaatgcaca cagcgcaccg aagccgccca gaccgcccat cacttccgga
   104161 cgacgcgttt tcttcactac gcctttgatt cttccaacca gagcattacc cgcgtcaata
   104221 tcaacaccgg catctttgta gctaagagag gttttatcgg tcactgcttg ggtccccacg
   104281 cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta acagggaaag caaacgtttg
   104341 cgagactgct ttacacaacc tttttgcacg tcttttcccc agacgcgcgg cgaaagaaga
   104401 cttgtgccag ggcaaaggtt agttttcgga tggaataatc ttctttcata accatttgaa
   104461 tataaaataa ctttatctca aaccgttatc attttgacta aagtcaacga aaagaatatt
   104521 gccgccttga agaaaggagg tataatccgt cgattttttt gtggctgccc ctcaaaggag
   104581 aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc tgggactgat
   104641 gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc gcttccgaag tgggtagcct
   104701 gctgacttac gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg aaggctggaa
   104761 cggcccggta gaaatcgacc agatcaaagg taagaaaatt accgttgtgc caattctgcg
   104821 tgccggtctg gggatgatgg acggtgtgct ggaaaatgtt ccgagcgcgc gcatcagcgt
   104881 tgtcggtatg taccgtaatg aagaaacgct ggagccggta ccgtacttcc agaaactggt
   104941 ttctaacatc gatgagcgta tggcgctgat cgttgaccca atgctggcaa ctggtggttc
   105001 cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc agcagcatca aagttctggt
   105061 gctggtagct gcgccagaag gtatcgctgc gctggaaaaa gcgcacccgg acgtcgaact
   105121 gtataccgca tcgattgacc agggactgaa cgagcacgga tacattattc cgggcctcgg
   105181 cgatgccggt gacaaaatct ttggtacgaa ataaagaata aaaataatta aagccgactt
   105241 taagagtcgg cttttttttg agtaaagcgc ctataacaca taatacagag gataatacta
   105301 tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact tttacagaca atcccgctta
   105361 gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc ttatttcata
   105421 ttaacccggc gactgtactg ttatttaacg gtattggaac gctgctgtat ctcttcatct
   105481 gtaaagggaa aattccggct tatcttggtt ccagctttgc ctttatttca ccggtattgt
   105541 tactgttgcc gttagggtat gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt
   105601 tctgcctggt ttcttttatc gtgaagaaag cggggaccgg ctggctggac gtgctgtttc
   105661 cacctgcggc aatgggcgca atcgttgccg tcatcggtct ggagctggcg ggcgtagctg
   105721 ccggtatggc gggtttactc ccggctgaag ggcaaacgcc agactccaaa accatcatca
   105781 tctctattac caccctggcg gtcacggttt taggttccgt gctgtttcgt ggtttcctgg
   105841 caattatccc gattttaatt ggcgtgctgg tgggctacgc gctctctttc gcaatgggaa
   105901 ttgtcgatac cacgccgatt attaatgctc actggtttgc gctgccaacc ctctatacgc
   105961 cgcgcttcga gtggtttgcc attctgacta ttctgccagc ggcgttagtg gttattgccg
   106021 aacacgtagg gcacctggta gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc
   106081 caggtctgca ccgttcgatg tttgctaacg gcctgtcgac cgtgatctcc ggcttctttg
   106141 gctctacgcc aaataccact tacggagaaa acattggcgt gatggcgatc acccgtgttt
   106201 acagtacctg ggttatcggt ggggcggcga ttttcgctat cctgctttcc tgtgtcggta
   106261 aactggctgc cgctatccag atgatcccat tgccggtgat gggcggcgtt tcgctgctgc
   106321 tttatggtgt catcggtgct tccggtattc gtgttttgat cgaatcgaaa gtggattaca
   106381 acaaagcaca gaacctgatc ctgacttccg tgattttgat catcggcgtc agtggcgcga
   106441 aggtaaacat cggcgcggcg gagttaaaag gtatggcgct ggcgaccatc gtcggtatcg
   106501 gcttaagttt gatcttcaaa ctgattagtg tgctgcgccc tgaagaagtg gtgctggacg
   106561 cagaagacgc ggatataaca gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt
   106621 caaacatcat gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta
   106681 tctctgaaca caccggcaca gctctctttg ccactttatc ttcctgacga cgaaaccttt
   106741 gcaagtttct ggccggggga taactcctct ttactggctg cgctgcaaaa cgtgctgcgt
   106801 caggaacata gcggttacat ctatctctgg gcacgcgaag gcgcggggcg cagccatctg
   106861 ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg
   106921 gataaacgca cctggtttgt tccggaagtg ctcgacggta tggagcattt gtcgctggtc
   106981 tgtatcgaca acattgagtg tattgcaggc gatgagttgt gggagatggc gattttcgat
   107041 ctctacaatc gaattctgga atcgggcaaa acacgattgt tgatcaccgg cgatcgtcca
   107101 ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc gactcgactg ggggcagatc
   107161 tacaaattgc agccactttc tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt
   107221 ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga
   107281 gaaatgcgca cgctatttat gacgttggat cagttggatc gtgcgtcgat taccgcgcaa
   107341 cgtaagctga ccattccgtt tgtgaaagaa attctgaagt tgtagataag gtgtttattg
   107401 tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt taatccatag tattcgtagg
   107461 ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg atggagcaca
   107521 tcttaaccca cgatttccag tacctgctcc ggtggacgac caatccgcgc tttgccattc
   107581 gcgaccacaa tcgggcgctc cattagcttc ggattatcca ccattgcctg aatcagcgcc
   107641 tcttcgctaa gcgaactgtc agccaggttc agttctttat aaagatcctc tttctggcgc
   107701 atcaattcac gggcgctatt catccccagc attttcagca aatcgcgcag cgttgccgca
   107761 tcggcgggtg tctcaaggta gagcaccact tctggctcca cgccgttttc tttcagcagg
   107821 ttcagcgttt cccggctctt tgaacagcgt gggttatggt agatttttac ctgtttggtc
   107881 attgttcttc ctttaatgcg aattacatct tggtataagg cttaaagcgt tcctgcagct
   107941 ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct aatttcacct
   108001 gcgaactggc gctactcaac agcgaaatgg cctgatcgag tcgtccggcg agcgcataac
   108061 cttctgctcg cgcagccagc tcctgatcac ggttatttag cgcggcttcc gcctgtgcca
   108121 gcaaatccca gccgttgctg tcatctttat tattaaaggt gtagcgattc agaatattgg
   108181 ccgcttcttg tggttgacca ccttgcagat aagcgttcgc caggttgagc tgcaacaccg
   108241 gattggtgcg taaatcgcgg gcatttttca ggcgattgat cgcctcattg gctttgtttt
   108301 gcccaagatc gatatcggta gccagatcga gataccatgc gttgccaggt tctgccgcca
   108361 gtaacggttg cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag
   108421 cacgaccata ttgtgctgct cgttgctgac gaacgtttcc tttcgcccat tcatccagca
   108481 aatcactggt gagctggtta cgtccggaat tatacatccc cagtgtgcgc gctttcgcca
   108541 gatagaaatc ttccgacgac tgcaccacca tcgggcgcat ctgattagca cggttgcggg
   108601 catctgccag acgactttcc ggcaacgggt gagtcaataa aatttccggc gggcgcgagg
   108661 agtaacgcgc ctgatcgagt aatttttcga ggaaggtagg catcgcctgc ggatcgaatc
   108721 ccgagcgttg cagcacctga ataccaatgc ggtccgcttc ctgttcattt tgctgggtga
   108781 aactgatcat cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc atccccgcct
   108841 gcggactggc catcgccagt aaaatagaac ctaacgcgcc gacccaggtc agcggcgcgc
   108901 tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt
   108961 gcgccataac tgaagccagt tgactttcgt tatcggaata acggaacagg gcagagtgca
   109021 gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg atcagaaaga
   109081 aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag acgcatcccc agcgaattaa
   109141 tatattgcgt taacagcggg tcattaatta acggcgcgct gccgcgtagc tggcggacat
   109201 aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca
   109261 tatccggcaa ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca atggtcatag
   109321 cagcaatgag ggttgcaacc aggttttttt tcaactgcct gaacataacc tctatcctgt
   109381 atttctgaag atagtcattt gaccgatact gtgaaatatc gttcccaccg cgccgccttt
   109441 gagtgtaacg gcgaagatta gcccactcaa atgacttttc agtttcctga atccgtcata
   109501 aaaagcgaag tcttttttgt gacatttcga tacaattgcg gatcgcaatc ccgctcttga
   109561 ggttcaggga aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc
   109621 gacccggaag cgattgcctt gctggttatt ttagttgccg gatttggcat tatctttttc
   109681 tttagtggcc tgcttgctcc gttgctggtg gctattgtgc tggcctattt gctggaatgg
   109741 ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct gggcgacgtc gattgtattg
   109801 gtggtttttg tcggtatatt gctactgatg gcgttcgtgg tactgcctat cgcctggcaa
   109861 cagggcatct acttaatccg cgatatgccg gggatgctca ataagctttc tgactttgcc
   109921 gccacgttgc cgcgccgcta tccggcgtta atggatgcgg gcattattga tgcaatggcc
   109981 gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc
   110041 tcgctggtcg gtttgctgac catagccgtc tatctggtgc tggtgccatt gatggtcttc
   110101 ttcctgctga aagacaaaga gcagatgctg aacgccgttc gtcgggtgct gccgcgcaac
   110161 cgtggactgg caggacaggt gtggaaggag atgaatcaac aaatcaccaa ctatatccgc
   110221 ggcaaagtgc tggagatgat cgtggtgggg atcgccacct ggctggggtt cttgctcttt
   110281 gggctgaact attcgctgct gctggcggtg ctggtcggct tctcggtact tattccgtac
   110341 attggcgcat ttgtggtgac cattccggtg gttggcgtgg cgctattcca gtttggtgca
   110401 ggcacggaat tctggagctg cttcgcggtg tatctgatta ttcaggcgct ggacggcaac
   110461 ctgttagtac cggtgttgtt ctccgaagcg gttaacctgc atccgctggt gattatttta
   110521 tcggtggtga tcttcggtgg tttgtgggga ttctggggcg tattcttcgc cattccattg
   110581 gcgacgctga tcaaagccgt gatccacgcc tggcccgatg ggcaaatagc gcaagaataa
   110641 tcttcagccc gacgaactgt ttcgccgggc tgatttttca ggattcctga cgtaaataaa
   110701 tagcccgata acacatactc accagcactg caccgccgat aatattaccc agcatcaccg
   110761 ggagcagatt ggcggtaata aaatggctga ccgtcagtgc aggaaaattg tctgcgctac
   110821 tgtgcgccag ttgccgaagg ggcgtggggc aaaatggcga atggcaatgg cgaacggaat
   110881 cacaaacaaa ttggcgatac agtgctcaaa gccactggcg acaaacaggg tgatgggcaa
   110941 aatcatagcg acgattttgt cgcataacga acgcccgcaa taactcatcc acagcgccag
   111001 gcaaaccatc agattgcaca taatgccgag gctgacagat tcagtaaatg tatgatgcat
   111061 tttgccctcg gcgcagtgta aaaccgcgac tccccacatc gcgttttcac tcatcaccag
   111121 cccggaaaac cagattaaca aactgaaaca ggcaatacct gccagattac cgcaggccac
   111181 cagaagtgcg ttaatcagcc aagttcgcca actaataacg ccccgacttt ttgccattac
   111241 cgtcattacc gacgaggtga acaggttggt gccgcaaacc gccagcaaaa taaagccaag
   111301 tgtaaagcaa aggccgccca ccagatgggt taatgcctgt gaagacgggg catcggcgat
   111361 aactgaaagg taaaaagtaa atccgatcgc cataaacacg ccagcggaca tcgccattaa
   111421 aaagaccgac actttactgc gggcaatttt atgggaggca atccgttcag cgattgccgc
   111481 ttttctggcg ctcaactgca agtcgaaaga gagtttgttt ctcatgcata ctcctgcaaa
   111541 agcagattac aacacctcgc gaaccgagat tcccagacgc tgcattcgtg acagcagcgt
   111601 ggtgcgcttc atccctaatc gtgtcgccgc gccacggggc ccggcaacaa tgccattggt
   111661 ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg tcattctctg gcgtcgtcgg
   111721 gtgcagcaac tgcgccattg aactgcgaag cgctgaatct tcatttagcg tcggcagtaa
   111781 acggctttgt cggacattaa gatgtaaatt cagactgtta ccgcgagtca gcagtaccgc
   111841 tcgctcaatc acgttttcca gctcgcgcac gttgcccggc cagtcccacg acatcaactg
   111901 gcgtagcgcc tcggtcggga tggcgtcaat tgaacgattc atatggcgcg ccattttttg
   111961 cgtgaaatgc tttgctaaaa gagggatatc ttccggacgg tcgcgcagcg gcggcaattc
   112021 cagtgggaag acattcaggc gataaaagag atcgctgcga aactggcgat cttcaaccat
   112081 ttgccacaaa tcacggttgg tggcggcaat gactctgaca tttaccggga tcgttctact
   112141 cccgccgaga cgctcaatct cccgttcctg caatacgcgc agcagtttag gctgaagttc
   112201 taacggcaga tcgccaattt catcgagaaa caacgtgccg ccatcggcaa tttcaaaacg
   112261 accacgatgg gtattaatcg caccagtaaa cgcccctttg tcatgaccga ataactcact
   112321 ttccagaaga ctggcgggga tggcagcgca gttgattttg accagcggct tgtcgcgtcg
   112381 cgggctaagt tgatggatcg ctctggcgat cacctctttg ccggttccgg tttcaccgca
   112441 aatcaacacc gtactgtcgc tcttcgccac aatatctacc tgctggagta ggtcttccat
   112501 tgcctggctt tgatagataa tgtcaccgac gcccagattc gaaaggagct gctcgctaag
   112561 ctggtggttt tcttgctgca aactttcctg caaatcggtc atgctacgct aggcatcggc
   112621 attgccaacg gcaatagcga tgcgatcggc tatgtgttgt agtagctggc agttttcctc
   112681 actaaagaga gtggaagagg tatgcgccag caacaatgca cccggtgtat ggttgccaaa
   112741 ggtaagcggt ataaggagcg cagattcgca gccgttagat gcaagtaaga gtaataacgg
   112801 gtcgcgttgc cagagaaaca gatcatctgc acggtgcgtc aaggtcggtt gtcgggtttg
   112861 tagcgtttgt gtcagcaata cactgtcgcc aggcatattg cgtggcagac acgcacaatg
   112921 tgaggcagaa agatcgctgc accacaggct gaacttctcg ttctttcgat gatcgcccag
   112981 taccatactg actgaagcca gaccgaaaaa atgatggatc tcacgagcga cgtcagcgat
   113041 cagatcgtcg agatcaagat gtgacagcac tgcattagtg atatccacca gtacccgatg
   113101 atgatcgcga tctttctcca gcaaacgcag cgccacctgc tgttcttcat cattgagcca
   113161 tgattgcaga accggggtga taatgcgtgc cagccagtta aacagtgtgc aatcctgggg
   113221 agtaaaacgg cagccgtggg tgcgaataaa gcgtagctcc gccattccac tactgccgag
   113281 aaggacggcg tgacgcgaat aatcgatcat ctcgccctga tactctgctg ttgccgtcac
   113341 atcgctgcca tcatcatggt agcggtaata gacgttgctt ttgagaacat gcagttcaat
   113401 aagttgtacc gggctgaacg gcggcggcag cgggatgaag gcgcgtaata aagacgcctt
   113461 accgtgtttc tgtgctaacc gctccgcgag cattccgttt accgcttcaa ttgttattcc
   113521 ttgcggcggg gcaaacatcg cctcgtctga catagccatc aacagtcctt attaatgaga
   113581 aatccttctc acgcaatttt tctggccatc agatagatgg ccggttcctg cacaatttca
   113641 tccaacagat gaatgaattg aatgctacgc gcctgccaca cagacgttgc gtcatctgcc
   113701 agtggcgcga atgcccgggt caacagggcg gtgtggctgg cgtcaataac aatctcacca
   113761 aaagtcatca cccccagcat cttacgtcgg gcttgctcct cttcgacctg ggcaagccaa
   113821 tcttcgtatt ccgtcagtgg gcagcggaag gcgacattca gacaatcaat cacgccaacg
   113881 tgatggccga tagccagcga gtaatacatt acctgagagc tgtgttccgg catctcgtcg
   113941 ctactggcga caaacttttt tcgcagcgtc cagaaaacaa tttccccgca ctcttcagtc
   114001 atgtaacccc cggagtacgc gaaagagttg ctgaacgata ctattgagac gtggatcgtc
   114061 ggcatcgcgc aaccaggtgt taacccgatt tcctgtagga tcgtcgctta aatggcgcag
   114121 gagccgatcg caaatttctc gcccctgacg atagcccgcc agccgccgcg cttctcgctc
   114181 aatggcgata cgctgtgatg gcgggatctg cggccacaac ggttgcatag tcaccccggt
   114241 gggatcgcga taatccaccg cgtgaatctt ctgttgcagc aaaccgagcg ccacggcgaa
   114301 accgtgaatg gtggcggccg gtgttggcgg gcagccgggg atccaaacat caatggggac
   114361 aatggtgtcg ctgccgcccc agacgctgta gagatcgtgg aaaatccgcc gccggcaccg
   114421 cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc ccgaagtgca
   114481 ggcatacgca tcgcccgggt gactgcgcca gtaaataaca aaatatcggc gtgacgcggt
   114541 gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata ctggtgtaat ggcggcaaaa
   114601 atttcgattt cacaggcgtt gcagccaccg caatcgacgc gataaacgta ggctgagcga
   114661 cggatatcct gtagcaaatg ccgcttcatc ttttgcgttt gctcgtccag cgtgatgggc
   114721 tggctgacat gttgtgtaag cactggactc atagctgctc cttagccacc agcggtacat
   114781 ctgtatcgtc gttgagcagc gtcgcgcgtt gtttgcactc cgggcagacg ctcgcttgcg
   114841 cccataacat ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat tcagcagcca
   114901 gtgcgacggt tttttgctgg gcaaacgggc gttcgcaacg gctgcaacgt tgtagatgga
   114961 acgtcgcgcg ggtatagaga tcggctttat tggtgacggt cagttcaaag ttattggtaa
   115021 gctggatggc tctggtcggg cacacttctt cacaacgtcc gcagtaaata caacgcccca
   115081 gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc agcgcatttg
   115141 ccggacaagc acaggcgcag gcaccgcagg caatacattg gctgggcatc aggtccggtt
   115201 tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc gtcgcggttc
   115261 cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc agcggcgaac
   115321 ggttacgatc aatgccgtag cgttcgatct ctttatacgg cacggttttt gactggcgct
   115381 tgcgcacatc taccagcgtc acacggtcgg tacaggagta gcagggatca aggctaccga
   115441 taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac accggccagt
   115501 tggcgtaggt ggcggcacgg cagcgccagc ggaacaattt ttggttatca ccgagcatgc
   115561 tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat gcgtgaggtt
   115621 tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgagg gcaaattcca
   115681 gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag aaaacatcgc
   115741 cgccggtaaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag gggtgatcaa
   115801 agcgcaaatc acgggcgatt tgtcggtcga gaatgccaat gccctgagtg cgttgttcca
   115861 tattcggcgt agcaagcagc atctctacca gctccgacac gtcggcgcgc atctcgcgca
   115921 ccagtttcag cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca ccaccaatca
   115981 gattcagacc gtaggtttta cgcgacccga tcagcaattc cgccatcgtc atcgactttt
   116041 cccgcactcg gaaaaattgc ataaagccgg tatcaaaacc aacgaaatgg caggagaggc
   116101 caaggttaag caaatgactg tgtagccgtt cgacttccag cagaatcgag cgaatagtat
   116161 gtgctcgttg cggcacctca atccccagtg cattttcaac cgaattggta taggccacac
   116221 tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa gaaggtcact tcgttgtagc
   116281 ccatccgcgt ttctgccagt ttctccatgc cgcgatggac ataaaacagg cggtaatcag
   116341 catcgacaat ttgctcgcca tccacaaaca agcggaagtg acccggttca tcggaggtga
   116401 tatgcagcgg gccgacaggg atcacccgcg catcgctgtt gccctcattg ataaacgcat
   116461 acgtttcgga atctgtcgtc ggttcagggc gcagtcgata atccatcgca tctttgcgca
   116521 gcggatgcat atcttccggc cagtcatcgg gcaacaccag gcgacgctga tccggcaggc
   116581 caaccggaat cagcccgtac atatcgcgaa tttctcgctc gccccagacc gcggcaggga
   116641 cgcgcggtgt gactgacgga aactcccgac tgtcggcatc gaccagtgcc ttcaccacga
   116701 tccagcattt ttcggcccct tccattgaaa gggcataata aaccgcgtaa tgaccgttaa
   116761 gtgtccgctc gtcgttgcca aacaggactg gaagccagcc atcatgttga taataaagat
   116821 aatgtacaac gtcaggcagc agattgattt tcaccgtaat ggtgacctgt tcaggcgttt
   116881 gtcgctcttc atccagcacc gcgccgggga actgcgtttt cagggcggcg agaatcgctt
   116941 cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct
   117001 gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg
   117061 actatagtgg aagcgcccgc caggatcctg atgacaggtt gtggaatatg cgttcccatc
   117121 gccagcatca tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc gagatcaccc
   117181 cggttaacgg cctgcggcgg tttcgccatt aacacccgcg cagccatccg taccaggccc
   117241 gccagcacca gcgttaacag caataacagc aggacgataa tcagcaggtg attacgtgcc
   117301 agtcctgcgg taacggtcat aaattcgcta agaaaaatgt tgaagggcgg catccctgcc
   117361 agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt gagcatccca
   117421 cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga accgcagaac
   117481 agcagcgttt ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa aattcccagc
   117541 ggcccgccaa tgcctagcgc caccgcgacc agccccatgt tctccacgct ggagtacgcc
   117601 agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc aaccgacaac
   117661 atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcttg gcaaataatg
   117721 atgtaatagc gaatcagcac caacagcgcg cagttcagca atacggcgga gagcagggcg
   117781 ctgaccggac tcggcgcttc actgtgagca tccggcagcc aggcgtgcat gggaaatagc
   117841 ccggttttgg taccaaagcc aattagcaca aacacaaagg ccaacagcat taatgttggg
   117901 tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc tgcctgcggc
   117961 ataacgctgg cggcgttggc gtataccagc acggtaccga acagaccaaa agcgacacca
   118021 acagtacaaa taatgatgta cttccatgct gattccagcg atgaacgctg accgtaaatg
   118081 cctaccagaa acgccgagct taaggtggtg gcttcgatcg ccgcccacat cacaatcagg
   118141 ttattgctgg taacaaccag cagcatggtg aacaaaaaca gatggaagaa accgtagtaa
   118201 tcgcacagtg taacgggtga aagctcgccg tgtgccactt catgacgcat gtagccaatc
   118261 gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa accgcccaga
   118321 ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc ggcagtttgg
   118381 accacccaga gtgccagaat cagcagcagt gtgatcccta agctatgtaa tacagtcacc
   118441 gtacaagtcg cagagagccc ccgtttccgg caggcaaaac agagcagcga aaaaagcagc
   118501 ggcgtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc ccttcagcgc
   118561 ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg ccagtaacac
   118621 catcacaatg acggcgaaga tggcgtcggt agcgatacct atttccacca gttccggtgc
   118681 tcgccaggca agaagcgcca gcaccagatg ggagccgttt tccatcaggc agtaaccaaa
   118741 aatttgccgc aggatattgc gctggctgac aatgcacagc aggccaagca gaaaatgacc
   118801 taacgctacc gccagcgccg gtttcagccc ggtagccatc ggtagcttca cgggctgaac
   118861 gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg ccggaccgaa
   118921 taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca tgattaacgg
   118981 taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt gctctgcggc
   119041 gaacaggcac gagagagtgg cgaaaataga caccagcacc agcgactggc aggcgtaaaa
   119101 tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca gcatcatcag
   119161 tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta ctgcaaccag
   119221 gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcaa caccaggcgc
   119281 attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc aggcacaacg
   119341 cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat cagcagaatc
   119401 atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcagc aaacagcggg
   119461 aatttactga agaagccgtt gaacggcggc acaccggtaa tcgccagcgc tgccacgcag
   119521 aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg caacaagcgc
   119581 gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt agcgaacgcg
   119641 tggttgacga tgtaggcgat gctgccctcc agtgccagcc gcccactttc accttcgatg
   119701 cggcgtggag ataggcactg atcggtgttg gcgcttccat tgcatccggt agccacgctt
   119761 gcatcggtag ctgggccgat ttcccccacg cggcaaacag gatgccgcca taaaccagat
   119821 aacgtgcgtc gccatgtaac tcgctcatcg cgctaagcac acacgttccg gtctgcaaaa
   119881 acagcgtggc ggcggcaaga tacaacccca acgaaccgat atgggtgata agcaacgctt
   119941 ttagtgctga acgctgcgct ttatcgctct ggtaataatt gatcaacgcc caggagcagc
   120001 cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt cgaggagagt accagtcccg
   120061 ccatcgcgcc gataaacacc agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt
   120121 gttcgcgatt tttatccgtc agataacccg tggagtagat cgtgaccagc aaaccgagaa
   120181 acaccaccac aaacagaatc agcgtactca cgcgatcaat gaccaggcca aacaatgcca
   120241 catcccccac gttgaccaac gtcaacgtga cggcaacttt atcggcctga taaaacgcgg
   120301 agatcagcga caacatgcac agcgtggtca gcgcggcgaa caaaaccccc cattcggcgg
   120361 cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaaggcagc aataacgtcg
   120421 tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa ccagaagacc
   120481 catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgaatgag taaaaaacgc
   120541 ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa aaccttgagc
   120601 agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag
   120661 gacagaaaca gggccacaaa cagtgatgcc atcacgacct gtttcagccc cagcccccat
   120721 ttcgctagcg ccagcccggc accggaatat tcggtcagcg ggccttcctg taattcctgt
   120781 tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc aaaaccacag
   120841 gccagtaacg ccagtacggt ggttagcggc gagttccagc ccatcgccag cgtattgctg
   120901 atcatctcga tatgcgtgga acctgctatc agcgccaata ccagcagtga gagaataagc
   120961 attggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc cgcaaacgga
   121021 cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag aagatagata
   121081 agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg taataaacag tggtaaggcc
   121141 atcgccagca ccagcatgct gctgattaat acccacggca tcaggcggaa catcagacct
   121201 gaagatgtcg gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg ataatcctgc
   121261 cagatccccg gcccgcggcg ggagtgcata cgcgcgcgta tctgccggga aatacccgta
   121321 aaaagtgggg ttagcatcag cagaatcacg gcctgtgcta acgcaaaaat aaccagatat
   121381 ccgtcgcaaa gagtttgtct catggtgatt tccttagacg gcaatagcga ttagcagcac
   121441 aaccagtgcg gcgaccacat acaggcaata gagacgaaag tcgccgcttt gcagatgctg
   121501 tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc gctcatccca
   121561 gaagggttct gtgctctgag ccctggcggt gacgtgcgca aaacctttat ttagcctcag
   121621 cgtagggtcg agctgttgtc gtagacgaaa taacgcagag aagaccacac gcagcggctg
   121681 catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc atgcccaggc
   121741 atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac cacagccagt aaaggccagg
   121801 cagcaaaggc atcgccagta gtaacagcaa cagtaatgag ggggtgacct gtgtatgaaa
   121861 cgtgccgggt acaagtgcta ttccgctggc gacagtgacg ggaggggtat tggtaaacgc
   121921 atgggcaata tgcattatct tcggtgccag ccaactggca ctaagcgcaa ttaatacgca
   121981 gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct gtgcctcttc
   122041 agcgtgtgta ctgcgcggcg caccacagaa agtaataccg tacattttta cgaagcacat
   122101 tactgccagc ccaccagtga ctgccagcat taccatagca ataggacccg caagttgtag
   122161 cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc attcgctgat
   122221 aaaaccattc agcggaggaa tggctgatat cgcgaggcaa ccaatcaggc atgctgcggc
   122281 tgtccacggc atccgtttcg ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg
   122341 cgaaataatc gcgcccgcgc cgagaaatag cagccctttg aacagcgcat ggttgagcag
   122401 atgaaacagt gcgccgagca gtccaaccac ggtgagcagc gggtcgtgca gtgacagacc
   122461 gaccatcgcc acaccgactg ccagcaaaat aatgccgacg ttttcgacgg tactccaggc
   122521 cagcagccgt ttgatatcct gttccgccag cgcatacagc acgcccagga gcgcggagat
   122581 tgcgccgatc gccatcacca gaatgcccca ccacagcggc aaacccgttt gcgccagcag
   122641 gtccatcgct actttcagga tgccgaaaat accgattttg accattacgc cagacatcaa
   122701 cgccgaagcg tgcgatggtg cggcagggtg agcgcgcggc aaccagctgt gcaacggcat
   122761 catcccggct ttcgcgccaa aaccgaaaaa ggccagcagg aacaccgccg acgccagccc
   122821 cggagaaagt gaaagcgtgc ggaaactggc aaaatcgagg ctgccgcttt cgcgccccat
   122881 cagcaagaag gcgatcataa tcagcaccga tccggcgtgg gcgataaaaa agtagagcat
   122941 gccagcgttg atcgacgttt tatcctgcct ggcaatgacc agaaacctgg aagacagcaa
   123001 catcatttca aacagcacga tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat
   123061 cgatgcgatg aaaagattca taaagaagcc catcgccgcc gcgcctttgc cctcgtattc
   123121 gcgcatataa gtcaatgaat agagcgaaca aaccaccacc agcaaggaga taaccagcac
   123181 cataaatgcc gagaggctat ccattcgcag ggttaaatcg gcaaagtcaa aaggggtggc
   123241 aaaatgggcg actaaagtta caccagcatg taattgcgta attccgctga tgatgccaat
   123301 cacgccgccc accagcgatg tgatgccgga aagcttaata gccagtctgt ccagaccgag
   123361 taaaaacagc gaagccagac tggcaaacag atagaggatc agcgaccagg ttaataattg
   123421 cagggcatcc attagtgttg ctcctgagtg agggaaaggg gatcctcccc gccattggca
   123481 aaccagcttg cggcaaggcg ctgtttttct ttcatctgac gttgcaggct atcgtcggtg
   123541 atcagccgta acgcctgatt cgggcaagcg cgaacgcagg ccggaccttc tggcaagaaa
   123601 tcacacaggt cgcatttcac cgcgacggtc tggacacccg gttcccagcg cagcaacgca
   123661 tgttggggca gcacgttttc ttcgccgtct ttgagtgagc cttcagcctg aaaaacatat
   123721 tgcgcatggg cattcaccgg acggcttcct gaagcgctga ttgcgccaaa tgggcagacc
   123781 acggcgcaaa gcttgcagcc aatacagagg ctttcgttga gttggatcgc atcatccctc
   123841 tgagagatgg cattgaccgg gcagacctgc aggcaagggg cttcctcaca gtgatgacac
   123901 acgacagggg cagtgattgt tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa
   123961 ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg tattacatcc tgtacaccac
   124021 agtggttcgg ccaccacaaa gcggttcatg tcggctcctt gaaaatgata tgaaacattg
   124081 catttcatat tgcataaagg atgccattct tattaactca atatattcaa taagttatgt
   124141 attgactgga ttctgaacgg tacaaaaatg atggtcatta acgttcagca aaaaatggat
   124201 tcgacacccg tgtcgcaggg gaaagcgaga atgtgatctt gatcacgaga agtgaggtat
   124261 gctggccgca agcgcagcca gcacggaatg gagcaaagta atcaggcgtg ttctttcagc
   124321 cagttcagca caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac atgttcgatt
   124381 ttgccatcag cgtcaatcag gaagctgatg cgatgaatgc catcgtaggt tttgcccatg
   124441 aaggactttt caccccagac gccgaattgt tcgcacacct ggtggtcctc atcagacagg
   124501 agcgtaaagt taagcagctc tttttccgca aaacgggaga gtttttcggg tttatcggtg
   124561 ctgataccca gcacatcaac gcccgctttt ttcaactcat ccatgttatc gcgtaagccg
   124621 caggcctgta cggtacagcc gggggtcatg gctttcgggt agaaataaac cagaacacgc
   124681 tgtccctgga agtcggtcaa attaacttgt tctccgtctt gatccggcaa gctaaatttc
   124741 ggtgcgatat caccggcttt cagtggattc attacttaac tccatcctgt tcatcatgtt
   124801 gggaataatt gacgacgtta atactgcctt gtgcattgag ttctgtacat agggctttga
   124861 acgcttgctc aatatttgct gcgtccgcag atgcggggct gtgggcggtt atctgaatat
   124921 gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg cgacaccagc tccgcaatgt
   124981 tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg gagtctgcca
   125041 catcgacctg aacccagaca gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct
   125101 tcatcacgat taaaagatcc agttcggcac ctttcaacgg taacgttgat tcaatcagag
   125161 taatggcatt ccatgaaccg gaaagcagca taataaacgt gaactcttct cccagcatcg
   125221 ccaggcgact gtcttcaata ttacagccgc aactactgac atgacgggtg atggtgttca
   125281 caattccagg gcgatcggca cccaacgcag tgatcaccag ataatgttgc gatgacagtg
   125341 tcaaactggt tattccttta aggggtgagt tgttcttaag gaaagcataa aaaaaacatg
   125401 catacaacaa tcagaacggt tctgtctgct tgcttttaat gccataccaa acgtaccatt
   125461 gagacacttg tttgcacaga ggatggccca tgttcacggg aagtattgtc gcgattgtta
   125521 ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt gaaaaaactg attgattatc
   125581 atgtcgccag cggtacttcg gcgatcgttt ctgttggcac cactggcgag tccgctacct
   125641 taaatcatga cgaacatgct gatgtggtga tgatgacgct ggagctggct gatgggcgca
   125701 ttccggtaat tgccgggacc ggcgctaacg ctactgcgga agccattagc ctgacgcagc
   125761 gcttcaatga cagtggtatc gtcggctgcc tgacggtaac cccttactac aatcgtccgt
   125821 cgcaagaagg tttgtatcag catttcaaag ccatcgctga gcatactgac ctgccgcaaa
   125881 ttctgtataa tgtgccgtcc cgtactggct gcgatctgct cccggaaacg gtgggccgtc
   125941 tggcgaaagt aaaaaatatt atcggaatca aagaggcaac agggaactta acgcgtgtaa
   126001 accagatcaa agagctggtt tcagatgatt ttgttctgct gagcggcgat gatgcgagcg
   126061 cgctggactt catgcaattg ggcggtcatg gggttatttc cgttacggct aacgtcgcag
   126121 cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg
   126181 ttattaatca gcgtctgatg ccattacaca acaaactatt tgtcgaaccc aatccaatcc
   126241 cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa
   126301 tgacaccaat caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt
   126361 tgctgtaaag tttagggaga tttgatggct tactctgttc aaaagtcgcg cctggcaaag
   126421 gttgcgggtg tttcgcttgt tttattactc gctgcctgta gttctgactc acgctataag
   126481 cgtcaggtca gtggtgatga agcctacctg gaagcggcac cgcttgcgga gcttcatgcc
   126541 ccggctggaa tgattttgcc ggtgacctcc ggtgattatg caatcccggt gaccaacggt
   126601 agtggtgctg tcggtaaggc gctggacatt cgtccaccag cccagccgct ggcactggtt
   126661 tctggcgcgc gtacccagtt cacgggcgat accgcttcat tgctggtgga aaatggtcgt
   126721 ggcaatactc tgtggccgca ggtggttagt gtgctgcagg cgaaaaacta caccatcacc
   126781 caacgtgatg atgctggtca gacactgacc accgattggg tacaatggaa ccgtctggac
   126841 gaagacgagc agtatcgtgg tcgttatcaa atctctgtta agccgcaggg ttatcagcag
   126901 gcggttacgg ttaaactgct gaacctggaa caggcgggca aaccggttgc agacgcggct
   126961 tccatgcagc gttacagcac ggagatgatg aacgttattt ccgccggtct ggataaatct
   127021 gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt
   127081 gcagctgatg acaccggttt accgatgctg gtcgtacgcg ggccgttcaa tgtggtttgg
   127141 caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg
   127201 cagggtaaca tggccgtaac ttataagccg ctgtctgaca gcgactggca ggaactgggc
   127261 gcgagcgatc caggcctggc atccggtgac tataaactgc aggttggcga tttagataac
   127321 cgcagcagcc tacagttcat cgatccgaaa ggtcatactc tgactcagag tcagaacgac
   127381 gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc tggaatcatc
   127441 cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg gcaggaaaac gcgattttag
   127501 cggtaattcg cacgaaattt gtttgtcgga cgtagttcgg ataaggcgtt cacgccgcat
   127561 ccgacaaaac atccggcaca ccagacagca aaagatttta aaacgttaat tcacacccag
   127621 gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa accgtataca
   127681 gcacggaaaa cccggacctg ttggtgctcg aattccgcaa tgatacgtca gcaggggatg
   127741 gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa caacaagttc aactacttca
   127801 tcatgagcaa actggctgaa gcgggtatcc cgactcaaat ggagcgtctg ctctccgata
   127861 ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg
   127921 ctgctggctc tctggtgaaa cgtcttggaa tcgaagaagg tattgagctg aacccgccgc
   127981 tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac gaatcttact
   128041 gcgaaacctt tggctgggtg agcaaagaga acctggcgcg tatgaaagag ctgacctaca
   128101 aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg tctgattctg gtcgacttca
   128161 agctggagtt tggcctgtac aaaggtgaag tggtactggg cgatgagttc tccccggacg
   128221 gcagccgcct gtgggacaaa gaaacactgg agaaaatgga caaagaccgt ttccgccaga
   128281 gcctcggtgg cctgatcgaa gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg
   128341 actgattttt ctggttgctt gtttctttag ccggagggta ctccggctaa atgtctttga
   128401 tacccccgct acaatacttc tcattatctc cctttgacag gaaagtcata ccaggccaat
   128461 atgtatatac ataacgtata tcccaaaaag ggggggctat ggaaactact gtatttttaa
   128521 gtaatcgcag tcaggccgtc agattaccga aagcggtcgc tctgccagaa gacgttaaga
   128581 aagtagaaat tattgcgatt ggtcgtacaa gaatcatcac tcccgccggt gagtcctggg
   128641 acagttggtt tgatggcgaa aatgtgagtg cggattttat ggatatccgt gatcaacccg
   128701 caatgcagga aagggaatca ttttgatgct gaaattcatg ctggatacca atatctgtat
   128761 tttcactatt aagaataaac cagtacatgt cagagagcgg tttaacctga attctggccg
   128821 gatgtgtata agttctgtaa cattgatgga gcttatctat ggggcagaaa aaagtcagat
   128881 gccagaacgc aatttggcgg ttattgaagg tttcgtatct cgtttggtag tgcttgatta
   128941 tgatagtgct gctgcgacgc ataccgggca aatcagggcc gagcttgccc ggcagggaag
   129001 gcctgttgga ccttttgacc agatgatagc gggacatgct cgcagtcgcg gtttgattgt
   129061 ggttaccaac aatacccgag agtttgagcg agtggcggga atcagactag aagactggag
   129121 ctaacattct cttttacttc taacattcgt aaattatata acgtggcatg aagccttaat
   129181 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
   129241 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
   129301 aatgttgaag acaggcgcaa cagctccggt ggtccgtcta tgggcggtcc tggttttcgc
   129361 ctgccaagcg gtaaaggcgg gctgatttta ctgttagtcg tgctggttgc aggctactat
   129421 ggtgttgatt taaccgggtt aatgactgga cagccggttt cccaacaaca atcaacgcgg
   129481 tccattagcc ctaatgaaga cgaagctgca aaattcacct cggtgattct ggcaaccacg
   129541 gaagacacct ggggacaaca gttcgagaag atgggtaaga cctatcagca accgaagctg
   129601 gtcatgtacc gtggaatgac gcgcaccggc tgcggggctg gtcagtccat tatggggccg
   129661 ttctattgcc cggcggatgg cacagtttat atcgatctct ccttctatga tgacatgaaa
   129721 gacaaacttg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
   129781 catcatgtgc agaaactgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
   129841 acgcaggcgg aagtgaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
   129901 ggtgtctggg ggcatagtat gcagcagcaa ggcgttctgg aaaccggcga tctggaagag
   129961 gcgctgaacg cggcgcaggc catcggcgat gaccgtttac aacagcaaag tcaggggcga
   130021 gtagtaccag atagtttcac acatggcact tctcagcaac gctacagctg gtttaaacgt
   130081 ggtttcgaca gcggcgatcc ggcacaatgc aatacttttg gtaaaagcat ttaacttccg
   130141 gggcagggat ggctgaactg actgcgcttc acacattaac agcgcaaatg aaacgtgaag
   130201 ggatccgccg cttgctggtg ttgagcgggg aagagggttg gtgttttgat catgcgctta
   130261 agttgcgtga tgccttacct ggcgactggc tgtggatttc gccgcagcca gatgctgaaa
   130321 accactgttc accctcggcg ctacaaactt tacttgggcg cgagttccgg catgcggtat
   130381 tcgacgcccg ccacggcttt gatgccgctg cctttgcagc acttagcgga acgttgaaag
   130441 cgggaagctg gctggttttg ttactccctg tatgggaaga gtgggaaaac caacctgatg
   130501 ccgactcgct gcgctggagt gattgccctg accctattgc gacgccgcat tttgtccagc
   130561 atctcaaacg cgtacttacg gcggataacg acgctatcct ctggcggcaa aaccagccgt
   130621 tctcgttggc gcattttact ccccgtactg actggcaccc cgcgaccggc gcaccacaac
   130681 cagaacaaca gcaactctta cagcagctac tgaccatgcc tccgggcgtg gcagcggtaa
   130741 cggctgcgcg tgggcgcggt aagtcggcgt tggcagggca actcatttct cgtattgcgg
   130801 gcagtgcgat tgtcaccgcg cccgcaaaag cggcaacgga tgtactggca caatttgcgg
   130861 gcgagaagtt tcgctttatt gcaccggatg ccttgttagc cagcgatgag caagccgact
   130921 ggctggtggt cgacgaagcc gcagccatac ctgcgccgtt gttgcatcaa ctggtatcgc
   130981 gttttcctcg aacgttgtta accactacgg tgcagggcta cgaaggcacc ggacgtggtt
   131041 ttttgctgaa attttgcgct cgctttccgc atttacaccg ttttgaactg caacagccga
   131101 tccgctgggc acagggatgc ccgctggaaa aaatggttag tgaggcactg gtttttgacg
   131161 atgaaaactt cacccataca ccacaaggca atatcgtcat ttccgcattt gaacaaacgt
   131221 tatggcgaag cgagccagaa acgccgttaa aggtttatca gttattgtct ggtgcgcact
   131281 accggacttc gccgctggat ttacgccgga tgatggatgc accagggcaa cattttttac
   131341 aggcggctgg cgaaaacgag attgccggag cgctgtggct ggtggatgag gggggattat
   131401 ctcaacaact cagtcaggcg gtatgggcag gttttcgtcg cccgcggggt aatctggtgg
   131461 cccagtcgct ggcggcgcac ggtagcaatc cactggcagc gacattgcgt ggacggcggg
   131521 tcagccggat agcagttcat ccggcgcgtc agcgggaagg cacagggcag caacttattg
   131581 ctggtgcttt gcaatatacg cgtgacctcg actatctttc ggtgagtttt ggttacaccg
   131641 gggagttatg gcgtttctgg caccgctgcg gttttgtgct ggtgcggatg ggtaatcatc
   131701 gtgaagccag cagcggttgc tatacggcga tggcgctgtt accgatgagt gatgcgggta
   131761 aacagctggc tgaacgtgag cattaccgtt tacgtcgcga tgcgcaagct ctcgcgcagt
   131821 ggaatggcga aacacttcct gttgatccac taaacgatat cgtgctttct gacgacgact
   131881 ggcttgaact ggccggtttt gcttttgctc atcgtccgct attaacgtcg ttaggttgct
   131941 tattgcgtct gctacaaacc agtgaactgg cattaccggc gctgcgtggg cgtttacaga
   132001 aaaatgccag tgatgcgcag ttatgtacca cacttaaact ttcaggccgc aagatgttac
   132061 tggtccgtca gcgggaagag gccgcgcagg cgctgtgcgc acttaatgat gttcgcactg
   132121 agcgtctgcg cgatcgcata acgcaatggc aattttttca ctgactcctt caattttctc
   132181 ccatggtcat tgtgagtcag cggcgtaaaa ttaatgacat cagttaagga gttcgccatg
   132241 aaacatgacc attttgttgt tcaaagcccg gataaacctg cgcaacagtt gttactgctt
   132301 ttccacggtg tcggggataa cccggtggca atgggggaaa taggtagctg gtttgcaccg
   132361 ctgtttcctg atgcgctggt ggtgagtgtc ggtggcgcgg agccgagcgg taatccggcg
   132421 gggcgtcagt ggttttcggt gcagggtatt acggaagata atcgccaggc gcgtgtggat
   132481 gcgatcatgc cgacgtttat tgaaacggtg cgctactggc agaaacagag cggagtaggg
   132541 gctaacgcca cggcactcat cggtttctct caaggcgcga ttatggtgct ggagagcatt
   132601 aaagccgaac cgggccttgc ttcacgcgtc attgctttta acggacgcta tgcgagcctg
   132661 ccggaaactg cgtctacagc cacgacaatt cacctgattc atggcggtga agatccggtt
   132721 atcgatctgg cgcatgcggt tgcggcgcag gaagcgttaa tcagtgccgg tggtgacgtg
   132781 acgctggata tcgtggagga tttaggccat gcgatcgaca atcgcagtat gcaatttgcc
   132841 ctggatcact tgcgttatac cattccgaaa cattatttcg acgaagcgct aagtggcggt
   132901 aagcctggcg atgatgatgt gattgaaatg atgtaaaaga cagattacct ggcctgataa
   132961 gacgcgtgag cgtcgcatca ggcatcgttc accattgccg gatgcggcgt taacgcctta
   133021 tccggcctac ggttgagcac tcagcttact tcttcggttg atcctttttc ggccagtcgt
   133081 cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg aagctccggc ttgttggcga
   133141 gaaatttctt atggtcgacg cgcttgagat ctttaatcac gttcagcagg acgcctacca
   133201 aaaagacaat caccagaatc caccaatatt tagccagcca gtccatgctt atttcctctt
   133261 gcagaaccac tcatcaggcg acgagctgtt ccatgatacg ttgatacata cgggcaagta
   133321 gctgcaggtc ggcagcgttc acacattcat taattttatg aatagtggca ttgaccggcc
   133381 cgagttccac cacctgcgcc cccatgcgag caataaagcg cccgtcggac gttccgcctg
   133441 tggtcagtag ctgcggttta atttcattat agtgctcaac cgcgttaacg accgcatcca
   133501 ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag ccaccagtcc accgtataac
   133561 gcagttgatg tttttcaagc agggcaagca cctgtgcttt gatcatctca tcagtcagtt
   133621 cggtgctgaa gcggaagtta aactgcacaa acagttcacc cggaataaca ttgttactgc
   133681 ccgttcccgc ctgaatatta gcaatctgca tactggtcgc cgggaaaaat tcattgccct
   133741 gatcccactc aatagccact aattcattaa ggaaaggtgc tgcgcgatgt actggattgt
   133801 cagccagatg cgggtaggcc acatgcccct gaacgccatg aatggtaagg ttgcaggtta
   133861 acgatccgcg acgaccattt ttcaccacat caccaaccac ttcgatactc gacggttcgc
   133921 cgaccaggca gtaatcgaga cgctcattac gtgccatcaa cgcttcgacg acttttaccg
   133981 taccgttgtg ggcactggct tcttcatcag aggtgatcag aaatgccagt cgccccgtat
   134041 ggttgggatg ttgtgcgaca aagcgttctg ccgctaccac catcgccgcc agcgagcctt
   134101 tcatatctgc cgcaccgcgt ccgaataaca tgccatcacg aatggtgggt tcaaacggcg
   134161 gattgatcca acgatcggcg tcgccaggcg gcaccacgtc ggtatgcccg gcaaaggcca
   134221 acgtttcacc ttgcccacgc catgcccaaa aattctgcgt atcggcaaaa tccatgcgtt
   134281 caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa agcctggcat cctgcgtcat
   134341 caggactcag ggaagggcgg cgaataagct gttgtgtcag ctcaataacc gggcacgaca
   134401 tagactacac ctcatggaaa aattgctgat aactggaatc actgaaaccc agcagcatag
   134461 gcttaccagg cgcgcagagc aatggacgtt tgataattgc aggcatttca gtcattaatg
   134521 ccgccgcaga ggccgcatcg gtgattttat tgcgggtggt ttcgtccagt ttacgccagg
   134581 ttgtaccacg ggtgttcagt aacgcttccc agcctaattc gttgataaaa ccgttcaata
   134641 attcgctgtc cagcccatcg acgcggtaat catgaaaacg atagtcgatg ttattggctt
   134701 ccagccaacg gcgagccttt ttaatggtgt cacaattttt gatgccgtaa agtgtaacca
   134761 taatgaatcc tttttcgatt tatgagaata aatactcatt taagggtgaa aacattttgc
   134821 ctgcaaatga ttatcaggaa atataacatt atcgaagtat tcaaattaac aactattaaa
   134881 taatattaaa catttttaac gtaaacatta agaatattca tcttgttatc tgtctgtatt
   134941 ttaaccagaa ttaaatcagt attagatgcc gtatgaatat ctcgaaatgt tgctgtgtat
   135001 cacatacaaa ttatcaccgc aggaccatag tatttacata aggtttgtcc cccggaggta
   135061 gtcatcgcag caatcgagta aattagcttc accacggtat attgtttccg tagaataccc
   135121 ataattataa gagaggttgt tatgattgaa cgtgaactgg ggaactggaa agactttatc
   135181 gaagttatgc ttcgtaagta attcccgaga caaagtataa acagcaagaa cccgctgagc
   135241 aggttcttaa tcggaaagtt gaaaaaggcg acacattggc atgtcgcctt ttttattgct
   135301 tattccgggc gcggcttcag cgggaagcgg cggcgcacca gcacaaagaa cagcggcacg
   135361 aagtaaatag ccagaatagt agctgaaatc atcccgccca ttacgccagt acccaccgca
   135421 tgctgaccac cggaaccggc acccgtgctg gttgccattg gcaatacgcc gaagataaat
   135481 gccagcgagg tcatcagaat cgggcgtaaa cgctgacggc aggcgtggag cgtcgcttca
   135541 aacaggtcgt ggcctttttg gttcatctcg ttagcaaact cgacgatcag gatggcgttt
   135601 ttcgccgata aaccaatgac cgttaacagg cccacctgga agtaaacgtc gttttccagc
   135661 ccgcgcatcc aggttgccag cagcgcgccg ataaccccca gcggcacgac cagcattacc
   135721 gagaacggta ccgaccagct ttcatacaat gccgccagac acaggaatac taccagcaag
   135781 gaaatggcgt acagcgccgg agcctgcgcg ccggaaagcc gctcctgata cgacatcgcc
   135841 gtccactcca gaccaaagcc gtttggcaac tgcttcacta acgattccat aatatccatc
   135901 gccgtaccgg tactgacccc cggtgcggct tccccaacaa tctcaaccgc agaataaccg
   135961 ttatagcgtt ccagacgcgg cgagcctgtc tcccagcgtg aggtcgcgaa agcagagaag
   136021 ggcaccatgc cgccatcttt atttcggaca taccagagat taatgtcatc tggcagcatg
   136081 cgatacggcg cagctgcctg cacatagact ttcttcacgc gaccgcgatc cataaagtca
   136141 ttaacatagc tcgaacccca ggcggtttgc agtgtgtcgt taatatcgtc gatatcaacg
   136201 cccagcgcct gagctttacg ctggtcgata tcaatctgca actgcggact gtcgtcgagg
   136261 ccgttatggc gcacacgggt tagctccggg ttttccgccg ccagcgccag caactggtta
   136321 cgtgctgcca tcagcgcatc gtgacctgct ccagcgtggt cctgcaactc catatcaaaa
   136381 cccgcagaac taccaagacc gctaattgct ggcgggctgc tggcgataac gcgagcttct
   136441 ttaatcttgt taaacgcctt tgttgcacgc tcgataatgg caaacgaggt gccggtctta
   136501 ctgtcgcgtt cgctccagtc tttcaggcgg ataaacattc gcgccacgtt ttgcccgtta
   136561 cccccggggc cagaaccaac agtggcaaac accgacatga tgttgtcttt ttcatgggtg
   136621 aagtaatatt tctcgatttg ctcaacgact ttcagggtct gttgttgggt tgaaccgctg
   136681 ggcaattgta ccgaggtagt aaacatgcca cggtcttcca gcggtaagaa cgacgtcggc
   136741 aaacgcagga acaggaacac catgccgcca agcagcagga cataaatcac aatccagcgc
   136801 aggctacggt ggagaatttt cgccacccct ttttcgtagc gttcggcgtt gcggttaaac
   136861 atctggttaa accaggcaaa aaagcctttt tgcccgtgat gctcaccttt tttcagtggc
   136921 ttaagtagtg tggcacacag agccggagtg aggatcatcg ctaccagtac tgacagcacc
   136981 atcgccgcaa caatggtaat agagaactgg cgatagatgg caccggtggt gccgccgaag
   137041 aaggccattg gtacaaatac cgccgaaaga accatcgcaa tcccgaccag tgccccctgg
   137101 atctgcccca tcgatttacg tgtggcttcg cgaggagtga gtccttcctc actcataata
   137161 cgttcgacgt tttccaccac cacgatggcg tcatccacca gcagaccgat cgccagcacc
   137221 atcgcgaaca tggttaaggt gttgacgctg taaccaaagg cgtaaagtac ggagaaggtt
   137281 cccatcaaca ccaccggcac ggcgatagtg ggtatcagcg tggcgcggaa gttttgcagg
   137341 aacagataca taacgaggaa aaccagagcg atagcttcca gcagcgtttt caccacgtct
   137401 tcaatggagg ctttaacaaa cgaggtggtt tcatacgcca ccttgtattc cagtccgtgc
   137461 gggaagtact gcgccagctc gtcgagacga ttgagtacca gctccgctgt cgccatttcg
   137521 tttgcgccgg aggccagttt tacccccagc ccggaggctg gcttaccatt gaagcggcta
   137581 agataatcgt atttctccgc ccccatttcg acggtggcga catcgcccag ccttacctct
   137641 gagccgtctt gattcacccg caaggtgata tcgcggaact gttctggtgt ttgcagcagt
   137701 gactgggcgt taatggtggc gttgagcgcc tgcttatcga cggaaggtgt accaccaagt
   137761 tgcccaaccg caatctgcgc gttctgtgac tcaatggcat cagtgacatc tttagccgtc
   137821 atctggaaac tgttgagttt cgccgggtcc agccagatac gcatggaata ttgcgaacca
   137881 taggcatcga tatccccgac gccattcacg cggcttaacg ggtcctgaat attactggca
   137941 acataatcgg caatatcctg tttatccatc gaaccatcgg tagagacgaa agcaatggtc
   138001 agaatattgg tatcgccggt tttacgcacc gtcacgccct gattttgcac cgcctgcggt
   138061 aactttcgca tggctgattg cagctggttt tgtacttgct gcacggcttc gtccggatcg
   138121 gtgcctgctt taaaacttaa agtgacagat gcctgaccgg tgccactgct ctgagatgac
   138181 atatacatga gattatcgag gccggtcata ttttgctcga taacctgggt cacggtgttt
   138241 tccagcgtct gggccgatgc gccgggatag ttagcggtca ctcgcacatt cggtggcgcg
   138301 agatcggggt attgttcaac gggcaatgag aaaatcgcca gggtacctgt cagacacaac
   138361 aggattgcca gcacccaggc aaaaatgggg cgatcaataa agaaattcgc cattaaaaga
   138421 ggacctcgtg tttctgcgta tcgttgtagg cttgacttgc ttcactgtag cggcaaggta
   138481 cgagccaatc gtggagaaaa aaaggagtta atgtaaatta tcatgccctt ttatactgaa
   138541 ccttaagtgc aagtattctt tggtcagtag gaggcattac atcagctgac tttcctcacc
   138601 ctccgcggag cgaaagctaa tactcactaa cgtgccgccg ccggaaggtt gtgaaaaagt
   138661 cagcgtccca cccagccgtt ccgcgcgttc gcgcatgata ttcagaccat aatgaccttc
   138721 gggctctttc ggttcaccga tgccaatccc gttatcacgg atataaaccg tgtgattgcc
   138781 gtccggcgcg gtgacgcaac tgacggcgat ttcgctggcg ttggcgtgct tcatcgcatt
   138841 cagcaccgct tcgcgaataa tttgcaacaa atgcacctgc atttgcgcat ccagcgccag
   138901 ggttggcaga cggcagtcga gggtcagttt ggcgctggtt tgattttgta acgtatccag
   138961 catttccctc aacgcggagg ggagatccgc ctgctgcagc gtcaggcgga aagtagtcaa
   139021 cagctcgcgt aactgccgat aagcatcatt caacgcctgg gaaaaatcgg ccatgatact
   139081 ttgtgcggtg gcgttatctt ccggtatcga acgcttcagt aacgtcaact ggatacgtaa
   139141 gtaagaaagt acctgagcca gcgagtcgtg caattcgcgg gcgatggttg cacgttcttc
   139201 catcaacaat aactgctgaa aatgcttctg cgcctgatta aagtacaaac cgcgtcccag
   139261 catcgacgaa acgctgttaa gcagcggttc tgatgatgaa acgtgactat tttgccagtg
   139321 cagttcgccg taaaccgtct cttgcattgt caccggtaaa atctgcatcg gcaattccgg
   139381 gtttggttgc ccttcgctaa tccgccagtt ttcaccgaca tttaactcca gatattcagc
   139441 cgcttcattg tcgcgaacaa tctgcaaaat atggcggaaa caatgcacat caatctggct
   139501 ggtgttcagc gcctgggaac attgatacaa cacctccaga cgacgcttgg cctcgtggag
   139561 atcgcgggtc ttttcttcta ctgacgcctc cagcgaaagg tacaatttat gcagctcgct
   139621 cgacatctgg ttaaaggttt ttgcaagcag accaagctca ttcggcagac tggtatccag
   139681 cggcggcgag tcgaactgtc cgtgttcaat acgctgactg gcggtaacca gctgattcag
   139741 cggggcaacc acctgatggc gtatgcggcg caaagtaaaa aagaccagcg tgaaaatacc
   139801 gataccgcca gccagggaaa tcgccaccac cagcagcatt ttgcgttcag cgtagtgctg
   139861 taaagccagc acgaacaggt ctatctgatt aacataatta ttaatattgg cctgatacca
   139921 cggcaaatcg cccttgctga gccgattatt catctccagc cagttggcat tcagatgcgc
   139981 atagcgagtt tttactgctt ctggcacata ccagacgttg agattggtta ataccggtga
   140041 atgcagtgcc tgctgaaaca actggcgatg tgcattgagt tgtggactgc cactttgcaa
   140101 gtcgtagccc aggcggtaac tctgcatacg cagcgatccg gcaatattga tagcctcagc
   140161 gtcgcgcaaa ctgctcgcca gagttagcag agcgataccc gtggaaagaa tcgacagcag
   140221 cacaatgtaa aaaaaggccc gggccagact ggccgagacg ggtcgtttaa caatcacacg
   140281 cgtcttctcc acaaaaaatc attaatgttc cagttcgctg aaacacaatt tagtcagtga
   140341 tgggattatt tgatctgcca cagtttatag cgaaatagtt acttattctg ggcacatgaa
   140401 cattgccgat tcccctgtaa gtcgggtaat aacaacactc atataaagaa taaggttttt
   140461 acaaccaaaa aagaaggtcg ttatgaatcg ttttattatg gccaacagtc agcaatgtct
   140521 gggttgtcat gcttgtgaaa tcgcctgtgt catggctcac aatgatgagc aacatgtcct
   140581 gagccaacac cattttcatc cccgaattac ggttatcaaa catcaacagc aacgtagtgc
   140641 agtgacctgt caccattgtg aagatgcgcc ctgcgcccgt agctgcccta atggcgcaat
   140701 cagccacgtt gatgacagca ttcaggtcaa tcagcaaaag tgtattggct gtaaatcctg
   140761 cgtggtggcc tgtccttttg gtacgatgca aatcgtcctg acacccgtcg cggcaggaaa
   140821 agtaaaagcc acggcgcata aatgcgacct ttgtgcgggg cgcgaaaacg gtcctgcctg
   140881 tgttgagaat tgcccggcgg acgcgctgca actggtcact gacgtcgcac tctccggcat
   140941 ggcgaaatcc cgccgcttgc gcaccgcgcg tcaggaacat caaccgtggc atgccagtac
   141001 cgcggcgcaa gaaatgccgg taatgagtaa agtcgaacaa atgcaggcaa cgcccgcgcg
   141061 tggcgagccg gataaactgg cgattgaagc gcgcaaaacc ggttttgatg aaatttatct
   141121 gccatttcgc gccgaccagg cacaacggga agcctcgcgc tgccttaagt gcggcgagca
   141181 cagcgtttgt gaatggacct gcccgctgca taaccatata ccgcagtgga ttgaactggt
   141241 gaaagccgga aacatcgacg ccgccgtcga gctttctcac cagaccaaca ccctgccgga
   141301 aattaccgga cgcgtttgtc cgcaagaccg tttgtgtgaa ggtgcctgta ctattcgcga
   141361 tgagcacggc gcggtaacta tcggcaacat tgaacgctat atttcagatc aggcgttggc
   141421 gaaaggttgg cgtcctgact taagtcatgt caccaaagtg gacaagcggg tggcgattat
   141481 cggggcaggt ccggcagggc tggcctgtgc ggatgttctg acccgcaatg gcgtggcggt
   141541 gacggtgtac gatcgccatc cagaaatcgg tggcttgctc actttcggca ttccttcttt
   141601 caaactggat aaatccctgc tggcacgccg tcgggaaatc ttcagcgcga tggggattca
   141661 cttcgaactc aattgtgaag tgggtaaaga tgtctctttg gattcgcttt tggaacaata
   141721 cgacgcggtc ttcgttggcg taggcactta ccgttccatg aaagcgggtt tacccaatga
   141781 agatgcgccg ggcgtttatg acgcgctgcc gttcctcatt gccaacacta aacaggtgat
   141841 ggggctcgaa gagctaccgg aagagccgtt tatcaatacc gccggactta acgtcgtggt
   141901 actgggcggc ggcgacaccg cgatggactg tgtgcgtact gcgctgcgcc acggcgcgag
   141961 taacgtcacc tgcgcttatc gtcgtgatga agctaacatg ccaggctcga agaaagaggt
   142021 gaggaacgcc cgcgaagagg gggccaactt cgaatttaac gtccagccgg tggcgcttga
   142081 gctgaatgaa caaggtcacg tctgcgggat tcgtttcctg cgcacgcgtc ttggcgagcc
   142141 ggatgcccag gggcgtcggc gacctgtacc gattgagggc agtgaatttg tcatgccagc
   142201 cgatgcggtg attatggcgt ttggcttcaa tccacacggg atgccgtggc tggagtcgca
   142261 cggtgtaacg gtagacaaat ggggccgcat catcgcggat gtggaaagcc agtaccgtta
   142321 ccagaccacc aatccgaaaa tcttcgccgg tggcgacgcc gtgcgtggtg cggatctggt
   142381 ggttaccgca atggcagaag gacgtcatgc ggcacagggg attattgact ggctgggggt
   142441 aaaatcagtc aaatctcact gatagcctgc gcagacaaac ccgacttcac agcgtaatat
   142501 aattgttcat ttcgcgctgt ggagtcggta tgacgcaaca aatcaccctc attaaagaca
   142561 aaattctctc cgataactat ttcaccctgc acaacattac ttacgatctc acgcgcaaag
   142621 atggcgaagt tatccgccat aaacgtgaag tttacgatcg cggtaatggt gcgacgatcc
   142681 tcctgtacaa cgcgaagaaa aagaccgtgg ttctgattcg tcagttccgt gtcgctacct
   142741 gggttaatgg caatgaaagc gggcagctga ttgaaacctg cgccgggctg ctggataacg
   142801 acgaaccgga agtgtgcatt cgtaaagaag cgattgaaga gacgggctat gaagtgggcg
   142861 aagtgcgcaa attatttgaa ctgtatatgt cgcctggcgg tgtgactgag ctaatccact
   142921 tttttatcgc cgaatacagt gacaatcagc gcgctaacgc cggtggcggt gttgaagatg
   142981 aagatattga agtgctcgag ctgccgttca gccaggcgct ggagatgatc aaaaccggcg
   143041 agatacgtga cggtaagacg gtgttattgc ttaactattt gcaaacgtca catttaatgg
   143101 actgaaaaat aacaataata tttcgttgtt tattattggc tcaatccgac aaatctgatt
   143161 gagccgcgct acctgcgcaa cgaagatacg acttgtgctg tttgtttgaa cttctggggt
   143221 cgtaccgtcc atgcgctatc gcattttcct tctctttttt ttcgctttgt tgccgacgtc
   143281 tttggtgtgg gcggcaccag cgcaacgggc gttttccgac tggcaggtca cctgtaataa
   143341 ccaaaacttc tgcgtggcgc gcaatacggg cgatcataat ggactggtga tgaccctgag
   143401 ccgcagcgcc ggggcgcata ccgatgccgt tttacgtatt gagcgcggcg ggttgaagtc
   143461 gccggatgcg tctgaaggag agatagcgcc acggctgctg ttagatggtg agccgttggc
   143521 gttaagtggt gacaagtggc ggatttcacc atggttatta gtaaccgatg atacggcaac
   143581 catcaccgcg tttttgcaga tgattcagga agggaaggcg atcaccttac gcgacggcaa
   143641 tcagaccatt tctctgagtg gcttaaaagc ggcgttgttg tttattgatg ctcagcaaaa
   143701 gcgcgttggc agtgaaaccg cgtggatcaa gaaaggggac gaaccgccgc tcagcgtacc
   143761 gcccgcgcct gcgctgaaag aggtcgcggt ggttaaccca acgccgacgc cactctcact
   143821 cgaagaacgc aacgttttgc tggattatgg aaactggcgg atgaatggtc tgcgctgctc
   143881 gcttgatccg ttacgtcgtg aggcgaatgt cactgcgctg actgatgata aagcgctgat
   143941 gatgattagc tgtgaggcag gggcctataa caccattgat ttggcatgga ttgtgtcgcg
   144001 taaaaagcca ctagcctcgc gcccggttcg gttgcgtttg ccgttcaaca acggtcagga
   144061 gacgaatgaa ctggaactga tgaacgcaac atttgatgag aaatcgcgtg aactggtgac
   144121 cttagcgaaa gggcgcggat taagcgattg tggcattcag gcgcgctggc gctttgacgg
   144181 tcagcgtttt cgcctggtgc gttatgccgc agaacccacc tgcgataact ggcatgggcc
   144241 agacgcctgg cccacgttgt ggatcacccg ttaaacaaaa ttgcggggac atgtaggccg
   144301 gataagacgc tgtaggccgg ataagacgcg tcagcgtcgc atccggcaat cagcatccgg
   144361 cgatcaccat caggcacctt tcactcccag caccttatgc gcttttgcca caatattctc
   144421 ggcggtaaag ccaaagaacg ggaacagctt atccgccgga gcagattccc cgtaacccgt
   144481 catcccgaca attgcccctt tcagaccaac atacttgtac cagtaatcgg caatacctgc
   144541 ttccaccgcc acgcgagccg caacgttaga aggcaacacc gactcccgat attcctcatc
   144601 ctgggcgtcg aaaatatcgg tcgagggcag ggaaactacg cgtacattgc gaccttctcc
   144661 tgctaatttc tctgccgctt gcagggtaat ttccatctct gaaccggtgg caatcaaaat
   144721 aatatctggc ttaccgccgc tgtctttcag cacataaccg ccacgagcaa tctctttaac
   144781 ctgatccggc gtacgttcca cctgggccag attctgcctt gagaggatca gtgccgtcgg
   144841 tccgttgtgg cgctcaaccg ccagcttcca gcccaccgcc gcttccacct gatcgcacgg
   144901 tcgccaggtg ctgaaatttg gcgttaagcg caggctggcc agttgctcaa cagcctggtg
   144961 cgtcggacca tcttcgccca ggccaattga gtcgtgggta taaaccataa tctgccgcgc
   145021 tttcatcagt gccgccatcc gcgcggcgtt acgggcgtat tcaacaaaca tcaggaacgt
   145081 cgcggtatac ggcacaaagc cgccgtggtg cgcgatgccg ttggcgatag cggtcatgcc
   145141 aaattcacgc accccgtagt gaatgtagtt gcccgctgga tcttccttca gcgaaacaga
   145201 acctttccag atggtcaggt tgctgggagc cagatccgcc gaaccgccga gcaactcagg
   145261 cagcatcggc ccgtaagcgt taagcgtatt ttgcgaagcc ttacgggtag cgattttcgc
   145321 cggatttgcc tgtaactcat tgatatattt ctgagtcgtt ttctcccagt ccttcggtaa
   145381 accaccgctc atccgtcggg taaactcttc tgccagttgc ggatgagcct ttttataggc
   145441 ggcaaacttc tcattccagc tctgctgcgc tttttcgcct ttttcacggg catcccaggc
   145501 gtgataaatc tttttaggga tctcaaatgg cggatggtgc cagcccagtt tttgccgtgc
   145561 cagcgccact tcttcttccc ccagtggtgc gccgtgcgcc tcttccttac ctgctttatt
   145621 cggcgaacca aagccaatca ccgtacggca gataatcagc gacggcttat ctttcacgct
   145681 ttgcgcttca aggatcgctt ccttcaccgc ctgcggatcg tgaccgtcga tttcatggat
   145741 cacatgccag tgataggctt caaaacgttt tgccgtatcg tcggtaaacc agccttctgt
   145801 ttcaccgtcg atggaaatac cgttgtgatc gtaaaaacca atcagcttgc ccagtcccag
   145861 cgtgcctgcc agcgaacaga cttcgtggga aataccttcc atcaggcagc cgtcgcccat
   145921 aaacacatag gtgaagtgat cgacgatctc atggtctggc tggttaaact gcgccgccaa
   145981 tgtacgttcc gctatcgcca gcccgacggc gttcgccaaa ccttgtccaa gcgggccggt
   146041 ggtggtttca acgcctgccg tatagccaat ctccgggtgg cctggggttt tcgaatgcaa
   146101 ctgacggaag ttcttcagtt cttccagcgg caggtcgtaa ccggtcagat gtagcaaact
   146161 gtagagcagc atcgacgcgt gaccgttgga aagaataaag cggtcgcgat cataccaggt
   146221 tgggtcggta gggttatgtt taagaaaatc gttccacagc acttcggcaa tatcagccat
   146281 gcccatcggc gcgccgggat gaccagaatt ggctttttgt accgcatcca tactgagtgc
   146341 gcgaatcgca ttggcaaggt cttttcggga catataacac tccgtggctg gtttatagtt
   146401 tggcggcaag aagatcttcc agtttgcgtt gatcaacagc gaacagacga atgccttcag
   146461 acagtttttc taccgccatc gcatcctgat tgtgctccca acggaactcc gcttcgctca
   146521 tgggagctgg gcgtgggaac gtctgagaag gtgggattaa tttacgtacc actggcgaaa
   146581 ctttttcctg cagctccttc agcaaattcg gtgcgatagt cagtcgatcg cagccggtta
   146641 aggcgaggat ttgttcggtg cgacggaagc tcgcgcccat cacaatggtt tcatagtggt
   146701 gttgcttata gtagtcgtag atattgcgca ccgatttaac gcccggatct tcttccacca
   146761 catacgggtc catcggcttg cgtgcctgat accagtcata aatacgcccg acaaacggcg
   146821 aaatcagaaa aacgcccgct tctgcacagg cccgcgcctg ggcaaaagaa aacagcagcg
   146881 tcaggttgca gttaatacct tctttttcca gctcttcggc cgcgcgaatt ccttcccagg
   146941 tcgaagccag tttgatcaaa atgcgtgatt tctcaacgcc ttgttgctga tacaagtcca
   147001 ccagatggcg cgccttctca atactctttt ctttatcaaa agagaggcgt gcatcaactt
   147061 ctgttgacac gcgaccgggt acgattttga ggatttcagc accgaaattg accgccagtt
   147121 tgtcacacgc tgcgaccacc tgttgttcct gggtcttgcc atttttttta ccccaggcga
   147181 tagcatcgtc tattaaatgc tcatattgtg ataatccggc agccttgagt aacagcgaag
   147241 gattggtggt ggcatcctgg ggatgataat ggcgaatgga ctcaatatcg ccgctgtctg
   147301 ccacgacagt ggtgaactgt ttgatgccgt ctaactcgtt cataggaaat actccttgaa
   147361 aagtaaagtg ttagatgagt gcgttaattc acacttctga gaaatttcgc taaacgcatc
   147421 aaaaaagcat agcagacagg catggtattg ctggattaag caggtaacat cagtgttata
   147481 ggattattac caaaacatta tattaatggt gagagtttgg acttgctcaa agtctgtaga
   147541 ctccggcagg gtaataatgt gcgccacgtt gtgggcaggg gctgttgccc acacacttta
   147601 tttgtgaacg ttacgtgaaa ggaacaacca aatggatgac cagttaaaac aaagtgcact
   147661 tgatttccat gaatttccag ttccagggaa aatccaggtt tctccaacca agcctctggc
   147721 aacgcagcgc gatctggcgc tggcctactc accaggcgtt gccgcacctt gtcttgaaat
   147781 cgaaaaagac ccgttaaaag cctacaaata taccgcgcgc ggtaacctgg tggcggtgat
   147841 ctctaacggt acggcggtgc tggggttagg caacattggc gcgctggctg gcaaaccggt
   147901 gatggaaggc aagggcgttc tgtttaagaa attcgccggg attgatgtat ttgacattga
   147961 agttgatgaa ctcgacccgg acaaatttat tgaagttgtc gctgcgctcg aaccgacctt
   148021 cggcggaatc aacctcgaag acattaaagc gccagaatgt ttctatattg aacagaaact
   148081 gcgcgagcgg atgaatattc cggtattcca cgacgatcag cacggcacgg caatcatcag
   148141 cactgccgcc atcctcaacg gcttgcgcgt ggtggagaaa aacatctccg acgtgcggat
   148201 ggtggtttcc ggcgcgggtg ccgcagcaat cgcctgtatg aacctgctgg tagcgctggg
   148261 tctgcaaaaa cataacatcg tggtttgcga ttcaaaaggc gttatctatc agggccgtga
   148321 gccaaacatg gcggaaacca aagccgcata tgcggtggtg gatgacggca aacgtaccct
   148381 cgatgatgtg attgaaggcg cggatatttt cctcggatgt tccggcccga aagtgctgac
   148441 ccaggaaatg gtgaagaaaa tggctcgtgc gccaatgatc ctggcgctgg cgaacccgga
   148501 accggaaatt ctgccgccgc tggcgaaaga agtgcgtccg gatgccatca tctgcaccgg
   148561 tcgttctgac tatccaaacc aggtcaacaa cgtcctttgc ttcccgttca tcttccgtgg
   148621 cgcgctggac gttggcgcaa ccgccatcaa cgaagagatg aaactggcgg cggtacgcgc
   148681 gattgcagaa ctggcccacg ccgaacagag cgaagtggtg gcttcagcgt atggcgatca
   148741 ggatctgagc tttggtccgg aatacatcat tccaaaaccg tttgatccgc gcttgatcgt
   148801 taagatcgct cctgcggtgg cgaaagcggc gatggagtcg ggcgtggcga cgcgtccgat
   148861 tgctgatttc gacgtctaca tcgacaagct gactgagttc gtttacaaaa ccaacctgtt
   148921 tatgaagccg attttctccc aggctcgcaa agcgccgaag cgcgtagttc tgccggaagg
   148981 ggaagaggcg cgcgttctgc atgccactca ggaactggta acgctgggac tggcgaaacc
   149041 gatccttatc ggtcgtccga acgtgatcga aatgcgcatt cagaaactgg gcttgcagat
   149101 caaagcgggc gttgattttg agatcgtcaa taacgaatcc gatccgcgct ttaaagagta
   149161 ctggaccgaa tacttccaga tcatgaagcg tcgcggtgtc actcaggaac aggcgcagcg
   149221 ggcgctgatc agtaacccga cagtgatcgg cgcgatcatg gttcagcgtg gggaagccga
   149281 tgcaatgatc tgcggtacgg tgggcgatta tcatgaacat tttagcgtgg tgaaaaatgt
   149341 ctttggttat cgcgatggcg ttcacaccgc aggtgcaatg aacgcgctgc tgctgccgag
   149401 tggtaacacc tttattgccg atacctacgt caatgacgaa ccagacgcag aagagctggc
   149461 ggagatcacc ttgatggcgg cagaaactgt ccgtcgtttt ggtattgagc cgcgcgttgc
   149521 tctgttgtcg cactccaact ttggttcttc tgactgcccg tcgtcgagca aaatgcgtca
   149581 ggcgctggaa ctggtcaggg atcgtgcgcc agatctgatg attgatggtg aaatgcacgg
   149641 cgatgcagcg ctggtggaag cgattcgcaa cgaccgtatg ccggacagcc ctttgaaagg
   149701 ttctgccaat attctggtga tgccgaacat ggaagctgcc cgcattagtt acaacttact
   149761 gcgcgtttcc agctcggaag gtgtgactgt cggtccggtg ctgatgggcg tggcgaaacc
   149821 ggttcacgtg ttaacgccga tcgcatcggt gcgtcgtatc gtcaacatgg tggcgctggc
   149881 cgtggttgaa gcgcaaaccc aaccgctgta attctttttt aactctcacg cttaccctga
   149941 atattcaggg taagcggttt ggctgcaata tattagtaaa gcttattact gagtttgcga
   150001 ataataaaaa aagcagtcta tataatatct cgatattatt ttatttatat tcatgcgttg
   150061 catatgaaag tttatgcacc acagcgaata tctctccatt cttagtgatc tacctcacct
   150121 tttaaacgcg cttgccgaat tttgttattt actctgacga aaaattgtca cgatacacga
   150181 aagtttttca caggcggcga ctcatggata aagaacgcat cattcaggaa tttgtgcccg
   150241 gcaaacaggt cacactggcg catctcatcg cgcatcccgg tgaggaactg gcgaagaaaa
   150301 tcggcgttcc ggatgcgggc gcaattggca ttatgacgct aactcccggc gaaacggcga
   150361 tgatcgccgg tgatttagcg ctgaaagccg ctgatgtgca tatcggtttt ctcgaccgct
   150421 tcagcggtgc attggtgatt tatggctcgg tgggcgcagt agaagaagcg ttatcacaaa
   150481 ccgtcagcgg tttaggtcgc ttattaaatt atacgctttg tgaaatgacc aaaagttaat
   150541 acgaggcatt catgaaacga attgcgtttg tgggtagcgt cggggcagga aaaacaacgt
   150601 tatttaatgc gttgcagggg aattataccc tcgccagaaa aacacaggcc gtggaattta
   150661 atgataaggg cgatattgat acgccgggtg aatattttag ccatccccgc tggtatcacg
   150721 ccttaattac cacgctacag gatgtagaca tgctgatcta tgtccatggc gcaaatgacc
   150781 cggaaagtcg gttgcctgcc gggttgctgg atattggcgt cagcaaacgg caaatcgccg
   150841 tcatcagtaa aacggacatg ccagatgctg acgtcgccgc aacgcgaaag ttattgcttg
   150901 agacgggttt cgaagagccg attttcgagc taaacagcca cgacccgcaa agcgtgcagc
   150961 aactggtgga ttatctggca tctctcacca aacaggagga agcaggtgaa aaaactcatc
   151021 acagcgaatg atattcgtga agcacacgcg cgcggcgaac aggcaatgtc cgtggttctg
   151081 cgcgccagca ttattacccc ggaagcccgt gaagtggcgg atctactggg ttttaccatc
   151141 accgaatgtg atgaatcgat cccggtaacg gcgtccgtgc ccgccagcgt gccagccgac
   151201 aaaaccgaaa gccagcgcat ccgcgaaacc atcattgccc agctgccgga aggccagttt
   151261 accgaaagcc tggtcgcaca gctgatggaa aaagtgatga aggaaaaaca gtcgctggag
   151321 cagggcgcaa tgcagccgag cttcaaatcg gtgaccggca aaggcggcat caaagtgatt
   151381 gatggcagta gcgtcaagtt tggtcgtttc gacggcgcag agccgcattg cgtgggcttg
   151441 actgatcttg tgaccggaga cgacggcagc agcatggccg ccggttttat gcagtgggag
   151501 aacgcattct tcccgtggac cctgaactac gacgaaatcg acatggtgct ggaaggggag
   151561 ctgcatgttc gccacgaagg ccaaaccatg atcgccaaag caggcgacgt gatgtttatc
   151621 ccgaaaggct ccagcatcga atttggtacg acatccagcg tgaaattcct gtatgtcgcc
   151681 tggccggcta actggcaatc cctatgaaag atttcatcac cgaagcatgg ctaagagcga
   151741 accatacgct cagcgaaggc gcagagatcc atctccctgc ggacagtcgc ctgacgccct
   151801 ctgcccggga gttgctggaa agccgccatc tgcgcatcaa gtttattgac gagcagggcc
   151861 gcctgtttgt tgacgatgaa cagcagcagc cgcagcccgt tcatgggctg accagtagcg
   151921 atgaacatcc gcaggcgtgc tgcgaactgt gtcgccagcc ggtggcaaaa aagccggata
   151981 cgctgactca cctgtcggcg gaaaaaatgg tcgccaaaag cgatccgcgt ctgggttttc
   152041 gcgcggttct cgacagcacc attgcgctgg cagtgtggct gcaaattgaa ctggcggaac
   152101 cgtggcagcc gtggctagcg gatatccgtt cgcgtcttgg caacattatg cgcgccgatg
   152161 cgctggggga accgctgggc tgccaggcga ttgtcgggct ttctgacgaa gatctgcatc
   152221 ggctttctca tcagccgctg cgctatctcg accacgatca tctggtgccg gaagccagcc
   152281 acgggcgcga tgcggcgttg ctcaatctgc tacgtaccaa agtgcgggaa accgaaaccg
   152341 tagcggcgca ggtgtttatc acccgcagct ttgaagtatt acgcccggac attttgcagg
   152401 cgctgaaccg cctttcgagc acggtctacg tgatgatgat cctgagcgta acgaagcagc
   152461 cgctaacggt gaagcagatc caacaacgac tgggggaaac gcaatgatta ttgaacgttg
   152521 tcgtgaactg gcgttgcgag cgcccgccag agtggttttt ccggatgcgt tagaccaacg
   152581 tgtgctgaaa gccgcgcaat atttacatca acaaggtctg gcaacgccca ttctggtcgc
   152641 caatccgttt gaacttcgtc agtttgcgct cagtcacggc gtggcgatgg acgggctaca
   152701 ggtgattgat ccgcatggca acctcgcaat gcgggaagaa tttgctcatc gctggctggc
   152761 ccgcgcgggc gaaaaaacgc cgccggatgc gctggaaaaa cttaccgacc ctctgatgtt
   152821 cgccgcagca atggtcagcg ccggtaaagc ggatgtctgt atcgcgggca acctctcttc
   152881 cacggcaaat gtgctgcgtg ccggattacg cattatcggc ttgcagccag gctgtaaaac
   152941 gctctcttcc attttcctga tgttgccaca gtacagcggc ccggcgttgg gctttgccga
   153001 ttgcagcgtg gtgccacagc cgacggcggc gcagctggcg gatatcgcgc ttgccagtgc
   153061 cgaaacctgg cgcgccatca ccggagaaga gccgcgcgtg gcgatgctgt cgttttccag
   153121 taacggtagc gcccgtcacc cctgcgttgc caacgtccag caggcgacag aaatcgtccg
   153181 tgagcgcgca ccaaagctgg tggttgatgg cgagttacag tttgacgccg ccttcgtgcc
   153241 ggaagtggcg gcgcaaaaag cgcctgccag cccgctacag ggcaaggcca atgtgatggt
   153301 ttttccgtcg ctggaagccg gaaatattgg ttacaaaatc gcacaacgac tcggcggata
   153361 tcgtgccgtc gggccactga tacaaggact tgccgcgccg atgcacgatc tctctcgtgg
   153421 ttgtagcgtg caggaaatta tcgagctggc gctggtggca gctgtgccgc gtcagacaaa
   153481 agtgaaccgc gaaagcagtt tacaaacact ggttgaatga acggtcccgt tctggaccct
   153541 gataaagagg aaagcacaat ggaagcatta ggaatgatcg aaacccgggg cctggttgca
   153601 ctgattgagg cttctgacgc gatggtgaaa gctgcgcgcg tcaagctggt tggtgttaag
   153661 cagattggcg gcggcctgtg tactgccatg gttcgtggtg atgttgcagc ctgtaaagcc
   153721 gcgaccgatg caggtgccgc agcggcacag cgtatcggcg agctggtatc tgtacacgtt
   153781 atcccgcgtc cgcacggcga cctggaagaa gtcttcccga tcggcctgaa aggcgatagc
   153841 agcaacctgt aatcaccctg tgtgacgtcg attcgggcgt cacgctctct cctttttcat
   153901 tttaccttct gcggagggta gggggagctt ttacctgaaa tacgccacgg aggcgggtat
   153961 gaaactggca gtcgtcactg gacaaattgt ttgtaccgta cgccatcacg gactggcgca
   154021 tgacaaattg ctgatggtgg aaatgattga tccacaaggt aatcctgacg ggcaatgcgc
   154081 cgtcgccatc gacaatattg gcgcgggaac cggggagtgg gtgttactgg tgagtggcag
   154141 ttccgcccgc caggcgcata aaagcgaaac gtcaccggtc gatctgtgcg tgattggcat
   154201 tgtcgatgag gtggtgtctg gcggtcaggt aattttccac aaataaggca gaacatcatg
   154261 aatcaacagg atattgaaca ggtggtgaaa gcggtactgc tgaaaatgca aagcagtgac
   154321 acgccgtccg ccgccgttca tgagatgggc gttttcgcgt ccctggatga cgccgttgcg
   154381 gcagccaaag tcgcccagca agggttaaaa agcgtggcaa tgcgccagtt agccattgct
   154441 gccattcgtg aggcaggcga aaaacacgcc agagatttag cggaacttgc cgtcagtgaa
   154501 accggcatgg gccgcgttga agataaattt gccaaaaacg tcgctcaggc gcgcggcacg
   154561 ccaggcgttg agtgtctctc tccgcaggtg ctgaccggcg acaacggcct gactttaatt
   154621 gaaaacgcgc cctggggcgt ggtggcttcg gtaactccgt ccactaaccc ggcggcaacc
   154681 gtaattaata acgccatcag cctgattgcc gcgggcaaca gcgtcatttt tgccccgcat
   154741 ccggcggcga aaaaagtctc ccagcgggcg attacgctgc tcaaccaggc gattgttgcc
   154801 gcaggtgggc cggaaaactt actggttact gtggcaaatc cggatatcga aaccgcgcaa
   154861 cgcttgttca agtttccggg tatcggcctg ctggtggtga ccggcggcga agcggtagta
   154921 gaagcggcgc gtaaacacac caataaacgt ctgattgccg caggcgctgg caacccgccg
   154981 gtagtggtgg atgaaaccgc cgacctcgcc cgtgccgctc agtccatcgt caaaggcgct
   155041 tctttcgata acaacatcat ttgtgccgac gaaaaggtac tgattgttgt tgatagcgta
   155101 gccgatgaac tgatgcgtct gatggaaggc cagcacgcgg tgaaactgac cgcagaacag
   155161 gcgcagcagc tgcaaccggt gttgctgaaa aatatcgacg agcgcggaaa aggcaccgtc
   155221 agccgtgact gggttggtcg cgatgcggcg aaaatcgcgg cggcaatcgg cctgaacgtg
   155281 ccgcaagaaa cgcgtttgct gtttgtggaa accactgcag aacatccgtt tgccgtgacc
   155341 gaactgatga tgccggtgct gcccgtcgtg cgcgtcgcca acgtggcgga tgccattgcg
   155401 ctggcggtga aactggaagg cggttgccac cacacggcgg caatgcactc gcgcaacatc
   155461 gaaaacatga accagatggc gaatgctatt gataccagca ttttcgttaa gaacggaccg
   155521 tgcattgccg ggctggggct gggcggggaa ggctggacca ccatgaccat caccacgcca
   155581 accggtgaag gggtaaccag cgcgcggacg tttgtccgtc tgcgtcgctg tgtattagtc
   155641 gatgcgtttc gcattgttta aggagtaacc gatggcgcac gacgaacaat ggctcacccc
   155701 acgtctgcaa acggcggcaa cgctgtgtaa ccagacgcct gccgcgacgg aatctccgct
   155761 gtggctgggg gtggatctgg gcacctgcga tgtggtgtcg atggttgtcg accgcgacgg
   155821 tcagccggtg gcggtatgcc tcgactgggc cgatgtggtc cgcgacggca tcgtctggga
   155881 tttcttcggc gctgtcacca ttgttcgtcg ccatctcgac acgctcgagc agcaattcgg
   155941 tcgccgtttt agccatgcgg cgacttcatt tcctccaggt acggacccgc gtatttccat
   156001 caacgtgctg gaatctgccg ggctggaagt gagccatgtg ctggatgagc caaccgcagt
   156061 cgcggatctg ctgcaactgg acaacgccgg tgtagtggat atcggcggcg gcactaccgg
   156121 cattgccatc gtgaaaaagg gcaaagtgac gtactcggcg gatgaagcca ccggcggaca
   156181 tcacatctct ctgactctcg ccggaaatcg ccgtatttcg ctggaagagg cggagcagta
   156241 caagcgcggt cacggtgaag agatttggcc tgcggtgaaa ccggtgtacg aaaaaatggt
   156301 ggacattgtc gcccggcata ttgaagggca gggcattact gatttatggc tggcgggcgg
   156361 ctcctgtatg caaccgggcg tggcggaact gtttcgcaaa caattcccgg cgttacaagt
   156421 gcatttaccg cagcacagtt tgtttatgac cccgctggcg atcgccagta gtgggcgaga
   156481 gaaagcggag gggctatatg caaaatgaat tgcagaccgc gctctttcag gcgttcgata
   156541 ccctgaatct gcaacgggta aaaacattta gcgttccacc ggtgacgctt tgcggtccgg
   156601 gctcggtgag cagttgcgga cagcaagcgc aaacgcgtgg gctgaaacat ctgttcgtga
   156661 tggcagacag ctttttgcat caggcaggga tgaccgccgg gctgacgcgc agcctggctg
   156721 ttaaaggcat cgccatgacg ctctggccat gtccggtggg cgaaccgtgc atcaccgacg
   156781 tgtgtgcagc cgtggcgcag ttgcgtgagt caggctgtga tggggtgatc gcatttggcg
   156841 gcggctcggt gctggatgcg gcgaaagccg tggcgttgct ggtgacgaac ccggatagca
   156901 cgctggcgga aatgtcagaa accagcgttc tgcaaccgcg cctgccgttg attgccattc
   156961 caactaccgc cggaaccggc tctgaaacca ccaacgtaac ggtgattatc gacacggtga
   157021 gcgggcgcaa gcaggtgtta gcccatgcct cgctgatgcc ggatgtggcg atcctcgacg
   157081 ccgcgctgac cgaaggtgtg ccgtcgcatg tcacggcgat gactggcatt gatgcgttaa
   157141 cccatgccat tgaagcgtac agcgcgctga acgccacacc gtttaccgac agtctggcga
   157201 ttggtgccat tgcgatgatt ggcaaatcgc tgccgaaagc ggtgggctac ggtcatgacc
   157261 ttgccgcgcg cgagagcatg ttactggctt catgtatggc gggaatggcg ttttccagtg
   157321 cggggcttgg gttgtgccac gcgatggcgc atcagccagg ggcggcgctg catattccac
   157381 acggtctggc gaacgccatg ttgctgccaa cggtgatgga atttaaccgg atggtttgtc
   157441 gcgaacggtt tagtcagatt ggtcgggcac tgcgaactaa aaaatccgac gatcgtgacg
   157501 ctattaacgc ggtaagtgag ctgattgcgg aagttgggat tggtaaacga ctgggcgatg
   157561 ttggcgcgac atctgcgcat tacggtgcat gggcgcaggc cgcgcaggaa gatatttgtc
   157621 tgcgcagcaa cccgcgtacc gccagcctgg agcagattgt cggcctgtac gcagcggcgc
   157681 aataaatgcc ggatgcgacg cttgccgcgt ctgatccggc cttcgggatt gcacatgtag
   157741 ggcggataag gcgtttacgc cgcatccgcc aataaataac aactaacagg gagtaaaggc
   157801 gatgggaatt aacgaaatca tcatgtacat catgatgttc tttatgctga tagctgccgt
   157861 agacaggatc ctgtcgcagt tcggcggttc tgctcgtttc ctcggtaagt tcggtaaaag
   157921 tatcgaagga tcaggcggtc agttcgaaga aggctttatg gcaatgggcg ctctgggtct
   157981 ggcgatggtc ggtatgaccg cgctggcacc ggtactggct cacgtactcg ggccggtaat
   158041 tatcccggtt tacgaaatgc tcggcgcaaa cccgtcaatg ttcgccggaa cgctgctggc
   158101 gtgcgatatg ggcggtttct tcctcgccaa agagctggcg ggcggcgacg tagcagcgtg
   158161 gctatactct gggttaattc ttgggtcgat gatggggcca acgattgtgt tttccattcc
   158221 ggtggcgctc ggcattatcg aaccttctga ccgtcgttat ctggcgctcg gcgtgctggc
   158281 gggcattgtg accattccga ttggctgtat tgctggtggt ctggttgcta tgtactccgg
   158341 tgtgcagatc aacggtcagc cggtggaatt cactttcgcc ctgatcctga tgaacatgat
   158401 cccggtgatc attgtcgcga ttctggtggc gctggggctg aaattcatcc cggaaaaaat
   158461 gatcaacggc ttccagatct tcgccaaatt cctcgttgca ttgatcaccc tcggtcttgc
   158521 cgctgcggta gtgaaattcc tgcttggctg ggaactgatc cccggtctgg atcctatctt
   158581 tatggcccct ggcgataaac ccggtgaagt gatgcgcgcc attgaagtta tcggttctat
   158641 ctcctgcgtg ctgttagggg cgtatccaat ggtgctgctg ctgactcgct ggtttgaaaa
   158701 accgctgatg agcgtcggta aagtactgaa tatgaacaac atcgcggcag ccggcatggt
   158761 ggcaacgctt gccaacaaca tcccgatgtt cggcatgatg aagcagatgg atacccgcgg
   158821 caaagtcatc aactgcgcct tcgccgtttc cgctgctttc gccctgggcg accacttagg
   158881 cttcgccgct gccaacatga acgccatgat cttcccgatg attgtcggca agttgatcgg
   158941 cggcgtaacg gcgattggcg tggcgatgat gctggtgcca aaagaagacg cgaccgcgac
   159001 taaaaccgaa gcggaggcac aatcgtgaac actcgccagc tattgagcgt cggtatcgat
   159061 atcggcacca ccaccaccca ggtgattttc tcccacctgg agctggttaa ccgtgcggcg
   159121 gtgtcgcagg tgccgcgcta cgaattcatt aaacgcgaaa ttagctggca aagtccggtg
   159181 ttctttaccc ctgtcgataa acagggcggt ttaaaagaag cggaactgaa aaccttaata
   159241 ctcgagcaat atcaggctgc gggtattgcg ccggaaagcg ttgattctgg tgccatcatc
   159301 atcaccggtg aaagcgcgaa aacccgcaat gctcgcccgg cggtgatggc gctctctcaa
   159361 tcgctggggg attttgtcgt tgccagcgcc gggccgcacc tcgaatccgt gatcgccggt
   159421 cacggagctg gggcgcaaac cctttctgaa caacggctgt gtcgggtact gaatatcgac
   159481 atcggcggtg gcaccgcgaa ctacgccctg ttcgatgccg gaaaaatcag cggcactgcc
   159541 tgtctcaacg tcggtggtcg cctgctggaa accgacagcc aggggcgcgt ggtttacgct
   159601 cataaaccgg ggcagatgat tgtggatgag tgcttcggtg caggcactga cgcccgttcg
   159661 ctgaccggcg cgcagctggt gcaggttacc cggcggatgg cggcgctgat tgtcgaagtg
   159721 attgacggaa cgctttcgcc gctcgcgcag gcattgatgc aaaccggttt gctgcccgca
   159781 ggtgttacgc ccgaaatcat tacgctttct ggaggcgtgg gcgaatgtta tcgccaccag
   159841 cccgccgacc cgttctgttt tgccgatatt ggcccacttc tggcaacggc gctgcatgac
   159901 catccgcgcc tgcgtgagat gaatgtgcag tttccggcgc aaaccgtacg cgccacggtg
   159961 attggcgcgg gtgcacatac cctttcgctc tctggcagca caatctggct ggagggcgta
   160021 caactgccgc tgcgcaattt gccggtggcg atcccgattg atgaaacgga tctggtgagt
   160081 gcctggcaac aggcgctgct tcagctggat cttgatccca aaactgacgc gtacgtgctg
   160141 gcgcttcccg cttcgctgcc tgtgcgttac gccgcggtac tgacggtcat caacgcgctg
   160201 gtcgatttcg tcgcgcgttt tccgaatccg catcccctgc tggtggtggc cgggcaggac
   160261 tttggtaaag ctctgggcat gttgttgcgc ccacagctac aacaactccc gttggcagtc
   160321 attgacgaag tgattgtccg cgcgggggac tatatcgaca ttggtacgcc tctttttggc
   160381 ggatcggttg tgccggtgac ggtgaaatca ctcgcatttc cttcctgagg gaacgactta
   160441 tgaaactaaa gaccacattg ttcggcaatg tatatcagtt taaggatgta aaagaggtgc
   160501 tggctaaagc caacgaactg cgttcggggg atgtgctggc gggcgttgca gcggcaagct
   160561 cacaggagcg cgtggcggca aagcaggtgt tgtcggaaat gaccgtagcg gacatccgca
   160621 ataatccggt gattgcctat gaagatgact gcgtgacgcg gctgattcag gacgatgtta
   160681 acgaaacggc ctacaaccag attaaaaact ggagcatcag cgaactgcgt gagtatgtgc
   160741 tgagcgatga aaccagcgtg gacgacattg cctttacccg caaagggctg acctcggaag
   160801 tggtcgcggc ggtagcgaag atttgctcca acgcggacct gatctacggc gcgaagaaaa
   160861 tgccggtaat caaaaaggcc aataccacca ttggtattcc gggcaccttc agcgcccgtt
   160921 tgcagccaaa tgacacccgt gacgacgtgc aaagtatcgc cgcgcaaatc tacgaagggc
   160981 tttccttcgg ggtgggcgat gcggtgatcg gcgttaaccc ggtgactgac gacgtggaaa
   161041 acttaagccg cgtgttggac accatttacg gcgtgatcga caaattcaac atcccaactc
   161101 agggctgcgt actggcgcac gtcaccaccc agatcgaagc gattcgtcgc ggcgcgcctg
   161161 gcggactgat tttccagagt atctgtggca gcgaaaaagg gctgaaagag tttggcgtgg
   161221 aactggcgat gctcgacgaa gcgcgcgcag tgggtgcaga gttcaaccgt atcgccgggg
   161281 aaaactgcct ctacttcgaa accggacaag gctctgcgct atccgctggc gctaacttcg
   161341 gcgctgacca ggtgacgatg gaagcacgta actatgggct ggcgcgtcat tacgatccgt
   161401 ttatcgtcaa caccgtggtc ggctttattg ggccggagta tctctacaac gaccgccaga
   161461 ttatccgtgc tggcttagaa gatcacttta tgggcaagct gagcggcatc tctatgggct
   161521 gtgactgctg ctacaccaac cacgctgacg ctgaccagaa cctcaacgaa aacctgatga
   161581 tcctgctcgc caccgcaggc tgcaactaca tcatggggat gccgctgggt gatgacatca
   161641 tgctcaacta ccagaccacc gcattccacg ataccgccac tgtgcgtcag ttactcaacc
   161701 tgcgcccgtc accagagttt gaacgctggc tggaaagcat gggcattatg gcaaacggtc
   161761 gcctgaccaa acgggcgggc gatccgtcac tgttcttctg atgacgcgga gataacacat
   161821 catggatcaa aaacagattg aagaaattgt acgcagcgtg atggcgtcaa tgggacaaac
   161881 ggcccccgcg ccgtcagaag caaagtgcgc caccaccaac tgtgcggcac cggtgacctc
   161941 ggaaagctgt gcgctggatt taggttccgc tgaagcaaaa gcgtggattg gtgttgaaaa
   162001 cccgcatcgc gcagacgtat taacagaact gcgccgcagc accgtggccc gcgtttgtac
   162061 cggtcgtgcc ggtccgcgtc tacgtaccca ggcgctgctg cgtttcctgg ccgatcactc
   162121 tcgttcgaaa gataccgtac tgaaagaagt gccggaagag tgggtgaaag cgcagggctt
   162181 gctggaagta cgctctgaaa ttagcgacaa aaatctctac ctgactcgcc cggatatggg
   162241 ccgccgcctg tgtgcagaag ctgttgaagc gctgaaagca cagtgtgttg ctaatccgga
   162301 cgtacaggtc gttatttctg atggcctgtc aactgatgcc atcaccgtga actacgaaga
   162361 gatcctgccg ccgctgatgg cgggcctgaa acaggccggg ctgaaagtcg gcacgccatt
   162421 ctttgttcgt tatggtcgcg tgaagattga agatcagatc ggtgagatcc tcggcgcgaa
   162481 agtggtgatc ctgctggtgg gggaacgtcc ggggttaggg cagtcagaaa gcctctcctg
   162541 ctacgccgtt tactcgccgc gtatggcgac caccgttgag gccgatcgca cctgtatctc
   162601 taacattcac cagggcggca cgccgccggt tgaagccgca gccgtcattg tggatttggc
   162661 gaaacgtatg ctggagcaga aagcatccgg catcaacatg acccgataag gaggcatcat
   162721 gccagcttta gatttgattc gaccgtcggt aaccgccatg cgggtgattg cctccgttaa
   162781 cgctgaattt gcgcgtgaac taaaattgcc gccgcatatt cgtagcctcg ggctgatttc
   162841 tgctgattcc gatgacgtca cctacattgc cgctgatgaa gcgaccaagc aggcgatggt
   162901 ggaagtggta tatggtcgct cgctgtatgc gggcgctgca cacggcccgt caccgactgc
   162961 cggtgaagtg ctgattatgc ttggtgggcc aaacccggcg gaagtgcgtg ccggtctgga
   163021 tgcgatggtt gcacatattg aaaatggtgc agcattccag tgggccaatg acgcagaaaa
   163081 caccgccttc ctggcgcatg tggtttcgcg taccggttct tatctctcat caaccgccgg
   163141 gatcacgctt ggcgatccga tggcatatct ggtggcaccg ccgctggaag cgacctatgg
   163201 cattgatgca gcgttgaaat ctgccgacgt acagctggtg acctatgtcc cgccaccgtc
   163261 tgaaaccaac tactcggcag catttttaac cggtagccag gccgcgtgta aagcagcctg
   163321 taacgccttt accgatgcag tgctggaaat cgcgcgtaat ccaatccagc gtgcgtaacg
   163381 gaggttgccg atgatcaatg cactgggatt gctggaagtg gacggcatgg tcgccgcgat
   163441 agatgcagcg gatgccatgc tcaaagcagc taacgttcgt ctgctcagtc acgaagtgct
   163501 tgaccctggt cgcttaacgc tggtggtgga aggcgatctg gcggcgtgtc gtgcggcgct
   163561 ggacgcaggt tgtgctgccg cgatgcgtac cgggcgtgtc atcagccgca aggagatcgg
   163621 tcggccagac gatgacaccc agtggctggt tactggcttt aaccgccagc cgaagcaacc
   163681 cgtaagggaa cccgacgcgc cagttatcgt cgcggaatct gctgacgagt tgttggcgct
   163741 gttaacatca gtacgtcagg gaatgacggc aggagaagtg gctgcccact ttggctggcc
   163801 gctggaaaaa gccagaaatg cgctcgaaca gctcttttct gccgggacgt tacgtaaacg
   163861 cagtagtcgt tatcgtctca agccccatta acctgtcgga ggtgccgggt gtcctacaac
   163921 acccggcatt aacatcatga aaaagacccg tacagccaat ttgcaccatc tttatcatga
   163981 acccttaccc gaaaacctga agctcacgcc gaaggtcgaa gtggataatg ttcatcaacg
   164041 acagacaacg gatgtctatg aacatgcttt aacgattacc gcctggcagc agatttacga
   164101 tcagctgcat ccgggcaagt ttcatggtga atttacggaa attctactcg atgatattca
   164161 ggtttttcgt gaatacaccg gtctggcgct gcgtcagtcg tgcctggtct ggccgaactc
   164221 gttctggttt ggcattccgg cgacgcgcgg tgagcaggga tttatcggtt cgcaatgtct
   164281 gggaagcgcg gaaatcgcca cccgccctgg tggcactgaa tttgaactga gcacgccgga
   164341 tgattacacg atcctgggcg tggtgctttc tgaagatgtc atcacccggc aggctaactt
   164401 tttgcataac ccggatcggg tattacatat gctgcgtagc cagtcggcgc tggaagtgaa
   164461 agagcagcat aaagccgcgc tgtggggctt tgtccaacag gcgctggcga cgttttgcga
   164521 gaacccggaa aatctccatc agcctgcagt gcgaaaagtg ctgggggata atttgctaat
   164581 ggcgatgggg gcgatgctgg aagacgcgca accaatggtg acggcggaaa gcatcagtca
   164641 tcagagttac cgtagattac tttcccgcgc ccgtgaatat gtgctggaaa atatgtctga
   164701 gccggtgacg gtgctggact tgtgtaatca actgcatgtc agtcgccgca cgctacaaaa
   164761 cgcgtttcac gctattttag gcattggccc aaacgcgtgg ctgaaacgca ttcgcctgaa
   164821 cgccgtacgc cgcgaactga taagcccgtg gtcgcaaagc acaacggtaa aagacgccgc
   164881 catgcagtgg ggattctggc atctggggca atttgccacg gattaccagc agctgtttgc
   164941 cgagaagccg tcgttgacgt tgcatcagcg gatgcgggaa tgggggtgag ggagttacac
   165001 ccaatccctg accttaataa actcacttaa cgccgcttct gggctgccat cttttggctg
   165061 ataatcatac tcccagcgta ccagtggcgg cattgacatc aggatcgact cggtgcgccc
   165121 gccagtttgc aggccaaaca gagtgccgcg atcccagacc agattgaact cgacataacg
   165181 accgcgacgg tagagctgaa aattgcgctc gcgctcgccg taggccatcg ctttacgtcg
   165241 ctctacaatt ggtaaataag cgtcggtgta gcctttgcct accgcctgca taaaggcaaa
   165301 acagtggtcg aaatctggcg tgttcagatc atcaaagaac agcccgccaa taccgcgctg
   165361 ttcgttgcga tgtttgaggt agaagtaatc gtcgcaccac tttttgtaac ggggataaac
   165421 gtcttcacca aatggcaggc acaggtcacg ggcggtgcgg tgccagtgaa tggcgtcttc
   165481 ttcaaaacca tagaaagggg ttaaatcgaa gccgccgcca aaccaccaga cgggctctgc
   165541 acccggtttt tcggcaataa aaaaacgcac attcgcgtgg ctggtgggaa catacgggtt
   165601 atgcggatgc actaccagtg aaacgcccat cgcctcgaaa ctgcgcccgg caagctccgg
   165661 gcgatgagcg gtggcggaag caggcatcgc ctcgccgtgg acgtgagaaa agttgacgcc
   165721 tgcctgttcg aaaacaccac cattacgcaa cacccgacta cgcccgccgc cgccagcttc
   165781 gcgctgccaa ctatcttcga caaattctgc gccatcgacg gcggtcagct gctgacaaat
   165841 cgtatcctga aggttgagca gaaactgttt aacctggtgt gcgtcgggtt tcataactta
   165901 tcgctttttc gaatgtgctt tctggttgtc gaaccagtgg aaataactga tcacgccttc
   165961 agcaatcgct gtggcgattt tctgacgaaa cgccgccgtg cctaacagcc gctcttcttc
   166021 cgggttggtg ataaacgagg tttccaccag caccgaagga accgacggcg atttcaacac
   166081 cacaaatgcc gcttgttcgg tgttgcggct gtgcagttta tgcaccggct taatcttctt
   166141 cagaatatgc gagccgagcg tcagactatt tttaatggta tctgtttgca ccagatcaaa
   166201 cagcacttgt tgcaataggt gatccttgtc agtcgccttt ttaccggcaa cttcatcggc
   166261 gcggttttcg cgttcagaca ggtatttcgc cattgcgcta cttgccccac ggttagagag
   166321 ggcaaatacc gaagcaccgg cagctttcgg gttggtaaag ccatcggcat gaattgacat
   166381 aaacagatct gcgccatgtt tatgggcgat ttcaacgcga tcgtaaagtg ggataaacgt
   166441 atcgccagaa cgcgttaaac gcgcatcaat cccatgatta cgcaaaatgg aacggacgtt
   166501 tttagcaatc gccagcacca catgtttttc tttcgaaccg ttgcgtccga tcgctccggt
   166561 atcaattccg ccgtgacctg gatcgagaac aacgacacgt ttgccgccag attttttggc
   166621 tttcggcttg ctgtgtccgt tgctggtttt taaaagttcg tctttggcga tggcttgcga
   166681 cattcctgac aacgtcaggg cagccaaacc ggctttcagc acctggcggc gcgaagtgag
   166741 tgtttttagt ggtttaaaag tgctcatacg gcctgagttg taataataaa gttccagatg
   166801 ttatatcgta tcgcgtaatc cgttacgacg gtttgatgtt gagaattgtt ttacttttca
   166861 tttcaatagt tgacaactcc ccattatgcc actttttcaa ccgattttcg tcagatattg
   166921 gctaattcga ccgttcacgc cataatcagc cttttaaccc gcaaaaaggt aacgaatacc
   166981 atggagatac gcgtatttcg ccaggaagat ttcgaagagg tcatcaccct ttgggagcgt
   167041 tgcgacttgc tgcgtccgtg gaacgatccg gaaatggaca tcgagcgtaa gatgaaccat
   167101 gacgtcagtt tgtttttggt cgctgaggta aacggtgaag tggtcggaac ggtgatgggc
   167161 ggttatgacg ggcatcgcgg gtctgcttat tatcttggcg tgcatccaga gtttcgtggg
   167221 cgtgggattg ccaatgcgtt gcttaatcgg ctggagaaaa agctgattgc tcgtggctgc
   167281 ccgaaaattc agatcaacgt gccggaagat aacgacatgg tgctcggaat gtatgaacgc
   167341 ctgggatatg aacacgccga cgtgctgagt ctgggtaagc gtttgattga agatgaagag
   167401 tactgagttt catcctgtcc attatgatgc gcacggtcgc ctgcgtttac ccttgctctt
   167461 ctggcttgtg ctattgcttc aggcgcgaac ctgggtgctg tttgtcattg ccggtgcgtc
   167521 gcgcgagcag ggtactgcgc tgctaaatct gttttatccc gatcacgata atttctggct
   167581 ggggctgatt cctggcattc cggcggtgct ggcgtttttg ttgagcggtc ggcgggctac
   167641 gtttcctcgc acctggcgtg tactctattt tctgttgctg ttggcgcagg tggttttact
   167701 ttgctggcaa ccgtggctgt ggctgaacgg tgaatccgtt agcggtatcg gtctggcgct
   167761 ggtagtggcg gatattgtgg cgttaatctg gctgctgacc aatcgacgtt tacgcgcttg
   167821 ttttaatgaa gaaaaagaat aacggcactt tttggtgaat ttgcactcca agcaacgtta
   167881 ttgaataacc aaaggaagtg acatgaaatc gctgcgttta atgttatgcg ctatgccgtt
   167941 gatgctgacc ggctgttcca cgatgtcgtc agttaactgg tcggccgcta acccgtggaa
   168001 ctggtttggg tcgtccacca aagtgagcga gcagggcgtt ggtgaattaa cggcgtccac
   168061 accactgcaa gaacaagcca ttgccgatgc gcttgatggc gattatcgcc tgcgcagcgg
   168121 aatgaaaacc gcgaacggca acgtggtgcg cttttttgaa gtgatgaagg gcgacaacgt
   168181 ggcgatggtg attaacggcg atcaaggcac gatcagtcgc attgacgtgc tggatagcga
   168241 tattcctgct gacaccggtg ttaaaatcgg tacaccgttt agcgaccttt acagcaaagc
   168301 atttggcaat tgccaaaaag ctgatggtga tgataatcgt gccgtcgaat gtaaagccga
   168361 aggcagtcaa catattagct accagttcag cggggaatgg agtggtcctg aagggttaat
   168421 gccttcggac gataccctga aaaactggaa agtcagtaaa attatctggc ggcgttaatt
   168481 tgcgtctgaa caaaccgccg ccgcgaaaaa acacgtaaaa tagcgctcaa caatgccacg
   168541 gattgcgtgg cattattatt ttcaggagga acaatgtctc aggttcagag tggcattttg
   168601 ccagaacatt gccgcgcggc gatttggatc gaagccaacg tgaaagggga agttgacgcc
   168661 ctgcgtgcgg ccagtaaaac atttgccgac aaactggcaa cttttgaagc gaaattcccg
   168721 gacgcgcatc ttggtgcggt ggttgccttt ggtaacaaca cctggcgcgc tctgagcggc
   168781 ggcgttgggg cagaagagct gaaagatttt ccgggctacg gtaaaggcct tgcgccgacc
   168841 acccagttcg atgtgttgat ccacattctt tctctgcgtc acgacgtaaa cttctctgtc
   168901 gcccaggcgg cgatggaagc cttcggtgac tgcattgaag tgaaagaaga gatccacggc
   168961 ttccgttggg ttgaagagcg tgacctgagc ggctttgttg acggtacgga aaacccggcg
   169021 ggtgaagaga cgcgtcgcga agtggcggtt atcaaagacg gcgtggatgc gggcggcagc
   169081 tatgtgtttg tgcagcgttg ggaacacaac ctgaagcagc tcaaccggat gagcgttcac
   169141 gatcaggaga tgatgatcgg gcgcaccaaa gaggccaacg aagagatcga cggcgacgaa
   169201 cgtccggaaa cctctcacct cacccgcgtt gatctgaaag aagatggcaa agggctgaag
   169261 attgttcgcc agagcctgcc gtacggcact gccagtggca ctcacggtct gtacttctgc
   169321 gcctactgcg cgcgtctgca taacattgag cagcaactgc tgagcatgtt tggcgatacc
   169381 gatggtaagc gtgatgcgat gttgcgtttc accaaaccgg taaccggcgg ctattatttc
   169441 gcaccgtcgc tggacaagtt gatggcgctg taagcgtttc tgattcattg attaaaggcc
   169501 aggcagtaac attactgact ggccttttta ttacttttgc tttaacgccg catacacctg
   169561 atcaactacc caaccatacg ttgcaatcgg cagctgaccg ctttcgaaca tattgggatt
   169621 cttttgcgga tcggcaaccg gcgaatgtgg cttgttgctt aacatcacaa tcgccatatg
   169681 attcaccgga tcgataacgg tcaccgttcc ggtccagcca gtgtgaccgt aggtttgcgg
   169741 gcttgccagc gtgccaaacg tcggcgtcat ggtggcatta ccattcacgc gccagccgag
   169801 gccaaaagtg gcatcttcct tagagctggt ggtgaacatc ttcaccgttt ccgcactgaa
   169861 cagctgcaca tcgccatagc ccccgccgtt cagcatcgtt tgcattaaca ccgcaatatc
   169921 gccggtattg gaaaacaaac ctgcgtgccc ggaaacgccg cccatcgaat aaaaggcttt
   169981 ttcatcgtgc acctgacccc agagagtgga ggtgcggata ttcgggaaat ggatcacgcc
   170041 atcgcgggta ttgccgttca attccgtggc ggcaatttgc tgcggtttaa agcctttcag
   170101 cagcgggtta aataccgtat gtgtcaggcc gagcgggcga taaatcgact cttcaacata
   170161 gcggtcaagc ggctgaccgg tgaccgactc gacgataaat ccaagcagca tataatcgac
   170221 atcactgtaa atatgcttgc tgccgggctg atactccagc ggcgtgcgct tgatcatctc
   170281 cagcgtctgg cctttatcct gggaatataa cgcgcccgcg accgctttat tcggatattg
   170341 tgggtccgcc gggaaaccgc cgctgtgatg cagcaggtca gaaatccgca gcgtgttttt
   170401 gcctttgatg gtgtcatttg gactatcggc aaaccccgga atatatttcg caatccgatc
   170461 gtcaggatgc agtttgcctt cgctcatcag cttttgcagg gcgaagttcg tggcgtacat
   170521 tttggtgttt gaggccaggt catacagcgt cccggtggtg gctttgacag gctgttccat
   170581 caacacgctg ccatcgtact ttttcgccgc tccccaggct ttgcgataca caatctgatt
   170641 atctttaatg atcagcaggt ttacgccggg ataaccggcg tccacttgct ggctaatcca
   170701 gcgatccatc tggttaagcc gttcgacgtt gaaccctgct ttctctggcg aactttctgt
   170761 cagaacaggg tattttgcgg cgctgacgct acaactaaca gccagcagag aaagatagag
   170821 cattgtccgt ttcatgatat cagtccagat tgacgttacg gcagccaaag agcgtggtga
   170881 aaataaaccc gcaaacccat gccaccagaa tcccgccagc ataaaccgcc attgccggaa
   170941 ggatgccttg tgcggaagtc atcagcggca gcgccaccag accagacggc ccaaaggcgc
   171001 tgtttaagcc catcggcaga ccccaccagg ctatcaggcc gataaacaaa ccgcccgctg
   171061 cgccgcctaa acaggcggta ataaacggtt tcatgcgggg cagggtgacg ccgtaaatca
   171121 acggttcacc aacgcccagc agaccgggaa taatcgcccc gcgtacctga ctgcgtaatg
   171181 cactgtgcgg ttgcgcccgc cagtagagtg ccagcgccgc gcccacctgg cccgcgcctg
   171241 ccattgaaag gatgggaaat aagctgttga atccctggct gtccattaac gcgaggtaaa
   171301 caggaataaa gccctgatgc acgccaaata ccacggcgat caggaacagc ccggctaaaa
   171361 ccgcacaacc gaacggatta ctgttcaggt gcataaacag ccatgacata ccttcgaata
   171421 accagccacc cagcggcata atgatcaggt acgcaagcgt agcggtgatc agcagggtga
   171481 ttaacgaggt cagcagcatg tcgagatcgt ccggcataaa gcggcgcacc atgccttcaa
   171541 tgcgggcgca ggcccacgcg gcaatcagca cgccgataat attgccgcgc ggatcgatgg
   171601 gcagaccaaa gaaatcgtga aaaccggcgt agtaaccggt ggttgcagcg gggttataac
   171661 cgagcaaaaa gagtgcggca ataattgcgc cattgacgcc cgtgccgcca aatgcctggg
   171721 cggcgttata gcccaccaga atcaccagga aagtgaacaa acctttgctg aacaccttca
   171781 taaaattcag cgcgtcgggt agcgtgccct gagcatctgc cggaacgtgc atcaccgtgg
   171841 caattaacgt cgctatcccc agcagcagac cggcggcaat aaaaccgggg atcagcggcg
   171901 tgaagatggt ggcgaatttg gcgagaaatt gttgcacgcc ggaggtttgt ttggctttta
   171961 actgacgttt gttctgggcg gcgatttctg cggcatcctg taccggtgcc tcgcccagca
   172021 gctcgctcat cgcttttgcg gcacgatgcg ctttaccagg cccaaaaacg acctgcacct
   172081 ggtcgctggt caaaatgacg cccttcacgc cttccagcgt tttgatattg ggatcaacca
   172141 gtgaactgtc atgtacaccc agacgcaggc gcgtcataca gttaccacaa ctggcgatat
   172201 ttcccggtcc gccgacacgg gtaagaatgg tgttcagaag ttcactgctg atctctttgg
   172261 ccatgggtta ttccttgtct aaaacctgac gaataaagcc gccgtgttga tccaggcgtt
   172321 ttttagcttc tgcggcatcg agatttttca gcaccatcac aatggccgtt ttacagttgc
   172381 gctcgcaggc aattaacgcc gcttccgctt gttcggcatt acatccggtg gcgtttttaa
   172441 cgatattgac ctggcgcaca tgcagttttt cgttggtggc gaccacatcg accatcaggt
   172501 tgccgaacac tttgccagat ttaatcatca gcccggtgga aagcatattg agcaccagtt
   172561 tctgcgctgt gcctgctttc attcgcgaag aaccagtgac cacttcggca cctacaatcg
   172621 gtgtaatggc aaactcggcg gtggtggaaa cggcactccc cggattacag gaaattccca
   172681 ctgtgcggca gccgagctgg cgtgcgtatt ccagtccggc aatcacatac ggcgtgcgac
   172741 cgctggcagc aatgccaacc accacatcct gtgccgttaa attaatattt ttcagatcgt
   172801 taacaccacc ttcccggcta tcttcagcgc cttccaccgc gtgctgaatg gcatattcgc
   172861 ctccagcaat caaaccaacc accagacccg gtttcacgcc gtaggtgggc ggacattcgc
   172921 tggcatccag aatccccaga cgaccggatg ttcccgcacc gagataaatc agacgaccgc
   172981 cgccgctgac ctgggcgtgg ataacatcga tcgccgcggc gatatccggc aggacgcgct
   173041 caacggcaag cggtacggtt ttatcttcat cgttgataat ccggcacatt tccagcgtcg
   173101 atacgcggtc aatttcagcc gaggcggtgt tcgagccttc agtaatcatc ttttctagtt
   173161 gcatcggtgg tgaccttact atttacgatc aaataattgc agatgtatgg aatcatatat
   173221 ccctaattga cagacttaaa ggaatcattg agtctacact cgcagagtgt gaagagcgtc
   173281 ataagaactt aagcggaatt tatgctgcca gcccaggaga gtggaagatg ttgtacctga
   173341 cgaaaataag caatgcgggg agtgaattca ccgaaaacga gcaaaaaatc gctgactttc
   173401 tgcaggccaa cgtgagtgag ctgcaatccg tttcttcacg gcagatggcg aaacagctcg
   173461 gcattagcca gtcgagcata gtgaaatttg cccaaaaact cggcgcgcaa ggatttactg
   173521 aattgcgtat ggcgttaata ggcgaataca gtgccagccg ggagaaaacg aatgccacgg
   173581 cgctgcacct gcacagttcg attaccagtg atgattcgct ggaagtgatt gcccgcaaat
   173641 tgaatcgcga aaaagagctg gcgctggagc aaacctgtgc gttgttcgac tacgcgcggc
   173701 tgcaaaaaat cattgaggtc atcagtaaag caccgtttat ccagataacg ggcctgggcg
   173761 gatcggcgct ggtggggcgc gatctgtcgt tcaaactgat gaaaattggc tatcgcgtgg
   173821 cctgcgaagc cgacacgcac gtacaagcca ctgtttccca ggcgttgaaa aaaggcgatg
   173881 tacaaatcgc tatctcttac agcggcagta aaaaagagat tgtcttatgc gcagaagcgg
   173941 cacgaaagca gggggcaacg gtgattgcca ttacttctct ggctgattct ccgctgcggc
   174001 ggctggcgca tttcacgctc gataccgttt ccggtgaaac cgaatggcgt agctcgtcga
   174061 tgtctacccg cacggcgcaa aattctgtta ccgatttact gtttgttggt cttgtgcaac
   174121 tcaatgatgt ggagtcattg aaaatgattc agcgcagcag tgagctaaca caacgcctga
   174181 aatgatgcat aaagcagcga ctggattgag attttcctga attagtgagc tgatccgcag
   174241 caatattttg tttatcctgt attttcagag ggaatggagt gtaacgctct gtattaacaa
   174301 ggagagcatt aaaatgggta aactcacggg caagacagca ctgattacgg gcgcattgca
   174361 gggaattggc gaaggaattg ccagaacttt tgcgcgtcat ggcgcaaacc taatcttgct
   174421 ggatatctcc cctgagatcg aaaagctggc ggacgaactg tgtggtcgtg gtcatcgctg
   174481 tacggcggtt gtcgccgatg tgcgtgaccc ggcgtcggta gccgcagcta tcaaacgcgc
   174541 gaaggaaaaa gaagggcgca ttgatatcct ggtgaataac gcaggcgttt gtcgtctggg
   174601 cagtttcctc gatatgagcg atgacgatcg cgatttccat attgacatca atattaaagg
   174661 cgtatggaac gtcacgaagg cggtgctgcc ggagatgatt gcccgcaaag atggtcgcat
   174721 tgtgatgatg tcttcggtca ctggcgatat ggtggccgat cctggcgaaa cggcgtacgc
   174781 cttaacgaaa gcggcgattg ttggcctgac aaaatcgctg gcggtggagt acgcgcagtc
   174841 tggtattcgc gttaacgcca tttgcccggg atacgtgcgc acaccaatgg cggaaagcat
   174901 tgcccgccag tcgaacccgg aagatccaga gtcggtgctg actgaaatgg cgaaagcaat
   174961 cccgatgcgt cgcctcgccg atccgctgga agtcggcgaa ctggcggcct tcctcgcatc
   175021 ggatgaatcc agctatttaa ccggtacaca gaatgtgatt gatggcggca gcacactgcc
   175081 ggagacggtt agcgtcggta tctgattcac ctctgtttcc tccctgcatt tgtggggagg
   175141 atttcgtttt aaacccggtt cttattccct tttcaacttc caaatcacca aacggtatat
   175201 aaaaccgtta ctcctttcac gtccgttata aatatgatgg ctattagaaa gtcattaaat
   175261 ttataagggt gcgcaatggc cgttaactta ctgaaaaaga actcactcgc gctggtcgct
   175321 tctctgctgc tggcgggcca tgtacaggca acggaactgc tgaacagttc ttatgacgtc
   175381 tcccgcgagc tgtttgccgc cctgaatccg ccgtttgagc aacaatgggc aaaagataac
   175441 ggcggcgaca aactgacgat aaaacaatct catgccgggt catcaaaaca ggcgctggcg
   175501 attttacagg gcttaaaagc cgacgttgtc acttataacc aggtgaccga cgtacaaatc
   175561 ctgcacgata aaggcaagct gatcccggcc gactggcagt cgcgcctgcc gaataatagc
   175621 tcgccgttct actccaccat gggcttcctg gtgcgtaagg gtaacccgaa gaatatccac
   175681 gattggaacg acctggtgcg ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg
   175741 ggtaacgcgc gttataccta tctggcggca tggggcgcag cggataaagc tgacggtggc
   175801 gataaagcca aaaccgaaca gtttatgacc cagttcctga aaaacgttga agtgttcgat
   175861 accggcggtc gtggcgcaac caccactttt gccgagcgtg gcctgggcga tgtgctgatt
   175921 agcttcgaat cggaagtgaa caacatccgt aaacagtatg aagcgcaggg ctttgaagtg
   175981 gtgattccga aaaccaacat tctggcggaa ttcccggtgg cgtgggttga taaaaacgtg
   176041 caggccaacg gtacggaaaa agccgccaaa gcctatctga actggctcta tagcccgcag
   176101 gcgcaaacca tcatcaccga ctattactac cgcgtgaata acccggaggt gatggacaaa
   176161 ctgaaagaca aattcccgca gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg
   176221 ccggaagtga tgaaaaccca cttcaccagc ggcggcgagt tagacaagct gttagcggcg
   176281 gggcgtaact gatgtttgct gtctcctcca gacgcgtgct gccgggcttt accttaagcc
   176341 tcggcaccag tctgctgttt gtgtgcctga ttttgctgct gccgctctcc gcgctggtga
   176401 tgcaactggc ccagatgagc tgggcgcagt actgggaggt gatcaccaac ccgcaggtgg
   176461 tcgcggccta caaagtaacg ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt
   176521 tcggtctgct gatggcgtgg atcctaaccc gctatcgctt cccaggccgc acgctgcttg
   176581 atgcgctgat ggatttaccc tttgcgctgc caacggctgt cgccggttta acgctggcct
   176641 cgctcttttc cgtaaacggt ttttacggtg aatggctggc gaagtttgat atcaaagtca
   176701 cctatacatg gctggggatt gcggtggcga tggcctttac cagcattccg tttgtggtgc
   176761 gtaccgtgca gccggtgctg gaagagttag gcccggaata tgaagaagcg gcggaaacgc
   176821 ttggtgcaac gcgctggcag agtttctgca aagtggtgct gccggagctt tctccggcgc
   176881 tggtggcggg cgtggcgctg tcgtttaccc gtagtcttgg tgaatttggc gcggtgattt
   176941 ttatcgccgg aaatatcgcg tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct
   177001 tgcaggagtt tgattacccg gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc
   177061 tgatactgct gttctcaatt aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc
   177121 attaatggcg gaagtgacac aattgaagcg ttatgacgcg cgcccgatta actggggcaa
   177181 atggtttctg attggcatcg ggatgctggt atcggcgttc atcctgctgg tgccgatgat
   177241 ttatatcttc gtacaggcgt tcagcaaagg gctgatgcct gttttacaga atctggccga
   177301 tccggatatg ctgcacgcca tctggctgac ggtgatgatc gcgctgattg ccgtaccggt
   177361 aaacctggtg ttcggtattc tactggcgtg gctggtgacg cgctttaact tccccggacg
   177421 ccagttgctg ctgacgctgc tggacattcc gtttgccgtg tcgccggtgg ttgccggtct
   177481 ggtgtatttg ctgttctacg gctctaacgg cccgctcggc ggttggctcg acgagcataa
   177541 cctgcaaatt atgttctcct ggccgggaat ggtgctggtc accatcttcg tgacgtgtcc
   177601 gtttgtggtg cgcgaactgg tgccggtgat gttaagccag ggcagccagg aagacgaagc
   177661 ggcgattttg cttggcgcgt ccggctggca gatgttccgt cgcgtcacat taccgaacat
   177721 ccgctgggcg ctgctttatg gcgtggtgtt gaccaacgcc cgcgcaattg gcgagtttgg
   177781 cgcggtgtcg gtggtttccg gctcgattcg cggcgaaacc ctgtcgctgc cgttacagat
   177841 tgaattgctg gagcaggact acaacaccgt cggctccttt accgctgcgg cgctgttaac
   177901 gctgatggcg attatcaccc tgtttttaaa aagtatgttg cagtggcgcc tggagaatca
   177961 ggaaaaacgc gcacagcagg aggaacatca tgagcattga gattgccaat attaagaagt
   178021 cgtttggtcg cacccaggtg ctgaacgata tctcactgga tattccttca ggtcagatgg
   178081 tcgcgttgct ggggccgtcc ggttccggga aaaccacgct gctgcgcatt atcgccgggc
   178141 tggagcatca aaccagcggg catattcgct tccacggcac cgacgtcagc cgcctgcacg
   178201 cgcgcgatcg caaggtcggt ttcgtgttcc agcattacgc gctgttccgc catatgacgg
   178261 tgttcgacaa tatcgctttt ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg
   178321 cagccatcaa agcgaaagtg acaaaattgc tggagatggt gcagcttgct catctggcag
   178381 atcgttatcc ggcacagctt tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc
   178441 tggctgtaga accgcaaatt ctgctgcttg atgaaccgtt tggcgcgctg gatgcgcagg
   178501 tgcgtaaaga gctgcgtcgc tggctgcgtc aactgcatga agagctgaaa ttcaccagcg
   178561 tgttcgtgac ccacgaccag gaagaggcga ccgaagtagc cgatcgtgta gtggtgatga
   178621 gtcaggggaa tattgaacag gctgatgcgc cgaatcaggt atggcgcgaa ccagcgaccc
   178681 gttttgtact cgaatttatg ggcgaagtga accgcctgca gggaaccatt cgcggcgggc
   178741 agttccatgt tggcgcgcat cgctggccgc tgggctacac acctgcgtat cagggtccgg
   178801 tggatctctt cctgcgcccg tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc
   178861 cgctgccggt acaggtactg gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc
   178921 agccgctggg gtggtacaac gaaccgctga cggtcgtgat gcatggcgac gatgccccgc
   178981 agcgtggcga gcgtttattc gttggtctgc aacatgcgcg gctgtataac ggcgacgagc
   179041 gtatcgaaac ccgagatgag gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa
   179101 tgttaaacgc ccggaggcgc ttcccgcgat ccgggctttt taatggcaag gtttgtaacc
   179161 tgtagacctg ataagacgcg caagcgtcgc atcaggcaac accacgtatg gatagagatc
   179221 gtgagtacat tagaacaaac aataggcaat acgcctctgg tgaagttgca gcgaatgggg
   179281 ccggataacg gcagtgaagt gtggttaaaa ctggaaggca ataacccggc aggttcggtg
   179341 aaagatcgtg cggcactttc gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg
   179401 ggtgatgtct taatcgaagc caccagtggt aacaccggca ttgcgctggc aatgattgcc
   179461 gcgctgaaag gctatcgcat gaaattgctg atgcccgaca acatgagcca ggaacgccgt
   179521 gcggcgatgc gtgcttatgg tgcggaactg attcttgtca ccaaagagca gggcatggaa
   179581 ggtgcgcgcg atctggcgct ggagatggcg aatcgtggcg aaggaaagct gctcgatcag
   179641 tttaataatc ccgataaccc ttacgcccat tacaccacta ccgggccgga aatctggcag
   179701 caaaccggcg ggcgcattac ccattttgtc tccagcatgg gaacaaccgg cactatcacc
   179761 ggcgtttcac gctttatgcg cgaacaatcc aaaccagtaa cgattgttgg cctgcagccg
   179821 gaagaaggca gcagtattcc cggcattcgc cgctggcctg cggaatatct gccggggatt
   179881 ttcaacgctt ctctggtgga tgaggtgctg gatattcatc agcgcgatgc agaaaacacc
   179941 atgcgcgaac tggcagtgcg ggaaggaata ttctgtggcg tcagctccgg cggcgcggtt
   180001 gccggagcac tgcgggtggc aaaagctaac cctggtgcgg tggtggtagc gatcatctgc
   180061 gatcgtggcg atcgctacct ttccaccggg gtgtttgggg aagagcattt tagccagggg
   180121 gcggggattt aagattattg cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt
   180181 aaaaaaggct gtgcgttctc atcgctctta ccggggatct tcacaatata cggcttcccg
   180241 gtaatcgatg acgacgaagc aaccttatca ataaactgct cggcagtgtc aatgcggtta
   180301 cgggtattgc ccagcttcag acgcagatga gaaaccgctt catcgcaggt atgttcatca
   180361 ccgttgcgca caaatatcaa atcctttttc tgtgctaatc cctccagcat ggcgttgatg
   180421 cgggcttctt catgggcagt taacttcgcg taagcgggga gtgtcattag tagtgtaatg
   180481 accagacaaa tgattttctt cattgaatta ccttatcctt gttttgtgat agccgatttg
   180541 actgccagaa tcaggtaaag tttcggttgg aatacgatga ggatcatcgg caaggagaaa
   180601 aaattaaatg agtagtttgt tgttgtttaa cgataagagc agggcgctgc aggcggatat
   180661 cgtcgccgtg cagtcgcagg tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc
   180721 tatcaaacag aacggtctga atgtttttgc cgtgccgacg gtattgctga gcaatacgcc
   180781 gcattatgac actttctacg gtggtgcgat tccggatgaa tggtttagcg gttatttgcg
   180841 tgcgcttcag gagcgtgatg cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg
   180901 aacggcatcg caaatcaaaa tccttgccga gtggctgact gcgctacgca aagaccatcc
   180961 tgacctattg atcatggtcg atccggtgat tggcgatatt gatagcggaa tttatgtcaa
   181021 acctgacctt cccgaggcgt atcgacaata tttactgccg ctggcgcagg gaattacccc
   181081 caatatcttt gagctggaaa tcctgaccgg taaaaattgc cgcgatctcg acagtgccat
   181141 tgctgccgca aaaagtctgc tttcagacac attaaaatgg gtggtgatta ccagcgcctc
   181201 cggtaatgaa gaaaatcagg agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt
   181261 catttcccat tcacgggtaa aaaccgacct gaaagggact ggcgacctgt tttgtgctca
   181321 gcttatcagt ggtttgctga aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt
   181381 gcgcgtactg gaagtgatgc gctacaccca gcagcatgag agcgatgaat tgattttgcc
   181441 gccgctggcg gaagcataaa aaatggcgcc gatgggcgcc atttttcact gcggcaagaa
   181501 ttacttcttg atgcggataa ccggggtttc acccacggtt acgctaccgg acagtttgat
   181561 cagttctttg atttcgtcca tgttggagat aacaaccgga gtcagggtag acttggcttt
   181621 ctcttccagt agcggcagat caaattcaat gacagtatcg ccaactttca cgcgctgacc
   181681 ttcttcagca atacgcttga agccttcgcc tttcagttca acggtgtcga taccgaagtg
   181741 gacgaacagt tcaacgccgc tatcagattc gatagagaac gcgtgattgg tttcaaagat
   181801 tttaccaatg gtaccgtcta caggcgcgac cattttgtta cccgttggtt tgatagcaat
   181861 accatcacca acgatttttt ccgcaaaaac gacatccggc acgtcttcga tattgacgat
   181921 ctcgccagag agcggagcaa tgatctcaat agttccggta tccttcttgt cgtcggaaac
   181981 cagagatttc agtttatcga acaaacccat gatcttctcc taagcagtaa attgggccgc
   182041 atctcgtgga ttagcagatt gttttttctt caatgaactt gttaaccagc gtcattaact
   182101 cgtccgttgt cggttgagca agagcctgct ctgctaacac cttcgcatct tcgaagttcg
   182161 tgttacggat aatcttctta atgcgcggga tagaaatggc gctcatagag aattcgtcca
   182221 gacccatccc cagcaacaga agtgtagcac gttcatcgcc agcaagctca ccacacatgc
   182281 cagtccattt gccttcagca tgagaagcat caataacttg cttgatcaag ttcagcacgg
   182341 acggtgacat tggctggtaa aggtgtgaaa tcatatcatt accacggtca actgccagag
   182401 tgtactgcgt taaatcattg gtgccgatac taaagaaatc aacttctttg gctaaatgac
   182461 gtgcaattgt tgcggcagcc ggtgtttcca ccattacgcc gatttcaatt gactcgtcaa
   182521 acgctttacc ttcgtcgcgc agttcctgtt tgtagatttc gatctctttg cgcagtgcac
   182581 gcacttcttc aacagagatg atcatcggga acataatgcg caatttaccg aaagccgagg
   182641 cacgcaggat agcgcggagc tgatcgcgca ggatctcttt acgatccatc gcgatacgga
   182701 tagcgcgcca gccgaggaac gggttctctt ctttcgggaa gttcatgtat ggcagctctt
   182761 tgtcgccgcc gatgtccatg gtacgaacga taaccgcctg cgagccacac gcttcagcca
   182821 ctgctttgta agcagcaaac tgttcttctt cagtgggcag cgcgtcgcgg tccatgaaca
   182881 ggaactcagt acgatacaga ccaacgcctt cagcgccgtt acgctctgca ccttcaacgt
   182941 cacgaaccgt accaatgtta gcgcacactt ctacctggtg accgtccagc gtaatagctg
   183001 gcagatcttt cagtttagca agctctgctt tttcagaagc cacttgctcc tgaacagcgc
   183061 gcattttatc aataacttcg ttggttggat tgacgtaaac ctgattattt acggcatcca
   183121 gaatcagata gtcgtcattt ttcacctgag aggtgacgct accggtaccc acgatagcag
   183181 gcagttccag agaacgcgcc atgatagagg tgtgggaagt acggccaccc gcgtcggtga
   183241 tgaaacccag caccttcttc aggttcagct gtgcggtttc ggacggcgtc aggtcagcgg
   183301 caaccagaat gacttcatcc tgaatggcgc tcaggtcgat aatcttcagg cccaggatgt
   183361 tgcgcagcag gcgcttaccg atatcacgta cgtcagccgc acgttctttc aggtattcat
   183421 catccagctc ttccagggca gaagcctgac cttcgataac ttcatgagca gctgcgtcag
   183481 ctgtcatgtg cttatcttta atcagggcta tgatttcctg ctccagctcc tcatcttcga
   183541 gcagcataat atgcccttca aagatggctt ctttttcttc accgaacgtt tcaccagctt
   183601 tcgttttgat cgtttccagc tgggctgatg ccttggcacg accgctcaga aaacgttcaa
   183661 cttcctgatc aacctggtcg gcagaaattt ttttccggtc aatgacaatt tcgtcttctt
   183721 tcagaagcag agctttaccg aaagcgatac ccggggatgc taaaatgcct gaaatcataa
   183781 ccctacctta cttgtgactg atttttaaaa gaacccggga aattactcga gttccgccat
   183841 cagtttaacc agatgttcaa ccgctttctg ctcgtcttcg ccttctgcgg agatagtcac
   183901 aacggtacct tgagtcaggc ccagagtctg cagtttaaac aggcttttcg cgctggcgct
   183961 tttgccgttg gaagtcacag taatttcaga agtgaagccc ttagcttctt ttacaaactg
   184021 ggcagcaggg cgggtgtgca gaccgttcgg agcggtaatg gtaacttctt gctggaacat
   184081 tgtatttccc caacttatag gtttagtgtt gtggaactaa agtctagcct ggcggttcga
   184141 ctttagcctg tattgttagc gccagttttt aacagacgcg acgcacgaag agcgcaatac
   184201 agtccattgc tggcgggtgt tgaccgctga cgtttcgtac gtcattaaac attatgccgc
   184261 taaaggaaga attgaaccaa atcataaaat cgattcagct ttggcttttc ctgtaacgat
   184321 taatttcgcg catcaaaata attagtctgg ttaaatacca gttccgacag gctgaatcaa
   184381 tgccaggagg ggcgaaagtt tgaagcaggc cacaaaaaag cacctaaaaa ggtgcttttt
   184441 tacgcatttt taacaagctg gcattactgt tgcagttctt tctcagtgaa gagatcggca
   184501 aacaatgcgg tgcttaaata acgctcaccc gatgacggta gaataaccac aatattcttg
   184561 ttggtaaagc tttcatcttc ttgtagtttc aacgccgcag caacagctgc tccagaagag
   184621 atacctgcaa gaataccttc ttcttccatc agacgacgcg cggtagaaat cgcttcttca
   184681 ttggtgatgc caatgacttt atcgaccagc ttgagatcga ggttagctgg gataaaacca
   184741 gcgccaatac cctgaatttt atgcgggcca ggtttaatct cttcaccagc cagtgcctgg
   184801 gcgataactg gagaatcggt tggctcaacg gcgacagaga taagatcggt cttgcctttg
   184861 gtgcctttaa tgtagcggct gacgccagtc agcgtaccgc cagtcccaac gccagcaata
   184921 aatacatcaa cctgaccgtc ggtatcttcc cagatctccg gaccggtggt tttttcgtga
   184981 atttcagggt ttgccggatt gctgaattgt tgcagcagca ggtatttctc tggattgctg
   185041 gcgacaattt cttctgcttt ttggattgcg cctttcatgc ctttagcacc ttccgtcagc
   185101 accaggtttg cacctaacgc tttcagcagc ttgcggcgtt caatactcat ggtttctggc
   185161 atggtcaggg tgagtttgta accgcgagcg gcagctacat aggccagtgc aatcccggta
   185221 ttaccgctgg tcggttcaac cagttcaacg cctggtttca gcacgccgcg cttttcggca
   185281 tcccaaatca tgttggcacc gatacggcac ttaacgctga agctggggtt acgagattcc
   185341 accttcgcca gaatgcgtcc gttaccgatg cgattcaggc gaaccagcgg cgtgtgaccg
   185401 atagtcagcg agttatcttc aaaaatctta ctcatggcct gtccttaact gtatgaaatt
   185461 gggatacaac aggtagcata cccgctcaga gaatatgcgg aagtaaggat ttagcatatc
   185521 tatatacaga agggaaataa tgacatgcaa gatggaataa ggggcggcat aagccacccc
   185581 tgtttcacac aaacggttta caaaataacc ggtagattgt taccgccaca tcgcgtgttt
   185641 atcgcgatag caatcgaccc acatcgccgt cgcgccacaa acggcaacgg gcatgatgaa
   185701 cagattaagc agcgggatca tcgtaaacag gctggttaaa gcaccaaact gcatattggt
   185761 gattttgcgt gtgcgcaggg cggtgcgcat ctctttaaac ggcactttgt ggttatcgaa
   185821 ggggtaatcg caatactgga tggctaacat ccaggcgcta aacaggaacc acagtaccgg
   185881 cgcgacggtt tgcccaatac cagggattaa gtaaagaatt agcaatacaa ttgcgcgcgg
   185941 cagataccag gcaaattttt gccattcgcg tttcatgatt cgcggcacat ctttcatgat
   186001 accgaaaatc ccggtatctg gcggtgtagc gccagtcaat cgtgcttcca gttgttcagc
   186061 caataaaccg ttaaacggag cggcaatcca gttagcaatc gtggagaaga aatagccaaa
   186121 cactaacagc acagagatga cagccagagg ccacaacaga taactcagcc attgtagcca
   186181 gtccggaacg taactcatga gagtcgggat ccagacatcg agctgtgtaa agagccacca
   186241 gaatgcgccc cccatcaaca aaatattgac cagcagcggt aaaataacga aacgccgaat
   186301 cccaggttgc gagacgagct tccagccttg cgcaaaatag taaaaaccgc tgcgtggggc
   186361 agatgtgaat gatgaaacca taatcaggat gagctccttt tgaccaatcc caggaaaatt
   186421 ctgcgtattt taccgggtaa ttgcgcaatg gacagttagg atatgttcga aaaaacagca
   186481 aaaagcacga tttcatctat ctttgtgctg tgaaagttaa tagtgcactt gcacttgagg
   186541 taatcggcaa atactcttag tgagtaaatg tttgccgtgg tggcaaggtg ttagaacaac
   186601 agagaatata atgatgcagg atttgcgtct gatattaatc attgttggcg cgatcgccat
   186661 aatcgcttta ctggtacatg gtttctggac cagccgtaaa gaacgatctt ctatgttccg
   186721 cgatcggcca ttaaaacgaa tgaagtcaaa acgtgacgac gattcttatg acgaggatgt
   186781 cgaagatgat gagggcgttg gtgaggttcg tgttcaccgc gtgaatcatg ccccggctaa
   186841 cgctcaggag catgaggctg ctcgtccgtc gccgcaacac cagtaccaac cgccttatgc
   186901 gtctgcgcag ccgcgtcaac cggtccagca gccgcctgaa gcgcaggtac cgccgcaaca
   186961 tgctccgcgt ccagcgcagc cggtgcagca gcctgcctat cagccgcagc ctgaacagcc
   187021 gttgcagcag ccagtttcgc cacaggtcgc gccagcgccg cagcctgtgc attcagcacc
   187081 gcaaccggca caacaggctt tccagcctgc agaacccgta gcggcaccac agcctgagcc
   187141 tgtagcggaa ccggctccag ttatggataa accgaagcgc aaagaagcgg tgattatcat
   187201 gaacgtcgcg gcgcatcacg gtagcgagct aaacggtgaa ctgcttctta acagcattca
   187261 acaagcgggc ttcatttttg gcgatatgaa tatttaccat cgtcatctta gcccggatgg
   187321 cagcggcccg gcgttattca gcctggcgaa tatggtgaaa ccgggaacct ttgatcctga
   187381 aatgaaggat ttcactactc cgggtgtcac catctttatg caggtaccgt cttacggtga
   187441 cgagctgcag aacttcaagc tgatgctgca atctgcgcag catattgccg atgaagtggg
   187501 cggtgtcgtg cttgacgatc agcgccgtat gatgactccg cagaaattgc gcgagtacca
   187561 ggacatcatc cgcgaagtca aagacgccaa cgcctgatac acttaaggca aattaactcc
   187621 tcttcgaacc cccgcttgtc gggggttttt agcattgatg gtgcgatatg gaatcaatcg
   187681 aacaacaact gacagaactg cgaacgacgc ttcgccatca tgaatatctt tatcatgtga
   187741 tggatgcgcc ggaaattccc gacgctgaat acgacaggct gatgcgcgaa ctgcgcgagc
   187801 tggaaaccaa acatccagaa ctgattacgc ctgattcgcc tacccaacgt gtaggcgctg
   187861 cgccgctggc ggctttcagc cagatacgcc atgaagtacc aatgctgtca ctggataacg
   187921 tttttgatga agaaagcttt cttgctttca acaaacgtgt gcaggaccgt ctgaaaaaca
   187981 acgagaaagt cacctggtgc tgtgagctga agctggatgg ccttgccgtc agtattctgt
   188041 atgaaaatgg cgttttagtc agtgccgcga cccgtggcga tggcaccacc ggggaagata
   188101 tcacgtctaa tgtgcgtact attcgcgcta ttccgctgaa gctgcacgga gagaatatcc
   188161 cggcgcgtct ggaagtgcgt ggtgaagtgt tcctgccgca ggcggggttc gaaaagatta
   188221 acgaagatgc gcgacgcacg ggcgggaaag tttttgctaa cccacgtaat gcggcagctg
   188281 gttcactgcg tcagcttgat ccgcgtatta cagcgaagcg accgctcact tttttctgct
   188341 atggcgttgg tgttctggaa ggtggcgagc tgccggatac tcatcttggc cgtttactgc
   188401 aatttaaaaa gtgggggttg ccggtcagcg atcgggtaac gctttgtgaa tcggcggaag
   188461 aagtgctggc gttctatcac aaagtggaag aagaccgccc gacgctgggc tttgatatcg
   188521 acggcgtggt gattaaggtc aactcactgg aacagcagga gcagcttggc tttgtcgcgc
   188581 gtgccccgcg ctgggcggta gcgtttaaat tcccggcgca ggagcagatg acctttgtgc
   188641 gtgacgtcga gtttcaggtt gggcgtactg gcgcgattac gcctgttgcg cgtctggaac
   188701 ctgtccatgt tgctggcgtg ctggtgagta acgcaacctt acacaatgcg gatgaaatcg
   188761 aacgtcttgg tttacgcatt ggcgataaag tggtgattcg ccgcgctggc gacgtgatcc
   188821 cgcaggtggt taacgtcgtg ctttctgaac gcccggaaga tacccgtgag gttgtattcc
   188881 cgacgcattg tccggtatgt ggttctgacg ttgagcgtgt ggaaggtgaa gcggttgccc
   188941 gctgtaccgg tggcctgatt tgcggagcgc agcgtaaaga gtcgctgaaa cactttgttt
   189001 cccgccgtgc gatggatgtt gacggaatgg gcgacaaaat catcgatcag ctggttgaaa
   189061 aagaatatgt ccatactccg gcagatctgt tcaaactcac cgcaggcaaa ctgaccggac
   189121 tggagcgtat ggggccaaaa tcggcacaaa acgtggttaa cgcgctggaa aaagcgaaag
   189181 aaaccacctt tgctcgcttc ctctatgcac ttggcatccg tgaagtcggc gaggccaccg
   189241 cagcaggtct ggcggcatat ttcggcacgc tggaagcgct ggaagccgct tcgattgaag
   189301 agctgcaaaa ggtgcctgat gttggcattg tcgttgcatc ccacgttcac aacttctttg
   189361 ccgaagaaag caaccgcaat gtcatcagcg agctgttggc ggaaggtgtt cactggcctg
   189421 cgccgatcgt tatcaacgcg gaagagattg acagcccgtt tgctggtaaa accgtggtgc
   189481 ttacgggcag cttaagccag atgtcgcgtg atgacgctaa agctcgactg gttgaactgg
   189541 gcgcgaaagt cgcgggcagc gtgtcgaaga aaaccgatct ggtgattgcc ggagaagcgg
   189601 caggttctaa actggcgaaa gcgcaggaac tgggcattga agtcatcgac gaagcggaaa
   189661 tgctgcgttt gctgggtagc tgagatggaa aaagagcagc tgattgaaat agccaatacg
   189721 ataatgccgt ttggcaaata taaagggcgt cgcttaatcg acctgccgga ggagtatctg
   189781 ctgtggtttg cccgcaaaga tgaattcccg gcagggaagc tcggtgagct aatgcaaatc
   189841 acgctgctga ttaaaaccga ggggctgacg caactggtcc agcccctgaa gcgtccgctt
   189901 taagctttat cggcgctgct ttcctgctgt gcctgtaact gttcggtctg gcgtttgtat
   189961 cgacgcgcca gcaccgcaca gaccatcaat tggatctgat ggaaaatcat caggggcagc
   190021 accatcatac cgatcaccga tgtggggaac agaatgtttg ccatcgggat gccatttgcc
   190081 agactctttt tcgaaccaca aaagacgata gtaatttcat ctgccttatt gaagcccagt
   190141 cggcgtgcca taaagacgtt aactacaatc acgatagcca gaagaacgca gctgaccacc
   190201 acgataaaca gcaatgatcc ccagccaact ttatgccaga taccattaac gacggcttcg
   190261 ctgaacgctg tataaaccac caacagaatg gacgtctggt cagttttcgc aatccatttt
   190321 ttattgcgcg acacccagtc accaatccac ggccgggaaa gatgccccaa cacaaacggc
   190381 agcagcagtt gcagcataat tttaccgacc tgctcaaggc tgccccctgc accgtgaaca
   190441 ttcatcacca gaccaaccag caatggtgaa aggaaaatcc ccagcaggct ggatgctgac
   190501 gcagaacaaa ccgccgccgc gacgttaccg cccgccattg acgtgaaggc gattgcagac
   190561 tgcacggtag ccgggagaat gcacaagtag agaaaaccgg agtagagcat cgggtcgaca
   190621 tttaccggtt tccaccaggc aaacagtaca cccagaatcg gaaacagcac gaaggtgctg
   190681 cacattaccc acaaatgcag tcgccagtga ccaccgccag caataatcgc ctcacgcgac
   190741 aacttcgcgc cgtgcataaa gaacagcagg gcaatcgctg cggtggtcag attttcaaag
   190801 aaggggacga aatcgcctct ggccggaaag aaagaggcca gcaacaccac cgtgatcagg
   190861 gttaaggtga aaggatcgag gatacgaaaa agtttcataa agactccaga tgatcgatgg
   190921 ttctattgtg cttcttccag tttgagaaat aaaattgatt tattgcatcc attcatgaag
   190981 aaaacagatg aattattctt taaaacaatt aaaggttttc gtcacagtag cgcaggagaa
   191041 aagttttagt cgtgcaggag agcgtatcgg cctgagccag tcggcagtga gtcacagtgt
   191101 gaaggaactg gaaaatcata ctggtgttcg cctgctggac agaaccacgc gtgaagtggt
   191161 gcttacagat gcagggcagc agctggcttt gcgtcttgag cgactgctgg atgaactgaa
   191221 cagcacgttg cgcgataccg ggcgtatggg gcaacaactg agcggaaaag ttcgggtcgc
   191281 tgccagccag accatttccg cgcatcttat tccgcaatgc attgctgaaa gccatcgccg
   191341 ctatccagat attcagtttg tcctgcacga tcgtccgcag cagtgggtga tggaaagtat
   191401 tcgccaggga gatgtcgact ttggcatcgt catcgatcca ggcccagtgg gcgatctgca
   191461 atgtgaagcg attctttccg agcctttctt tctgctttgc catcgcgata gcgctttggc
   191521 cgtcgaagat tatgtaccct ggcaggcatt acagggagcg aaattggtgt tgcaggatta
   191581 cgcgtcaggc agccgaccgc tgattgacgc agcgctggcg cgcaatggta ttcaggcgaa
   191641 tattgtgcag gagattggac atccggcgac gctgttcccg atggtagccg caggcatcgg
   191701 catcagtatt cttcccgcac tggcgctacc attgcctgaa ggtagcccac tggtggtaaa
   191761 gcgcattacg ccggttgttg aacgccagtt gatgctggtg cgcaggaaga accgttcact
   191821 ttccaccgcc gctgaagcgt tatgggacgt ggtacgagac cagggcaatg cgctaatggc
   191881 tggtcgggaa ggggatccgt tgtatcagat atagagtgaa ttcatactgc cgggagcccc
   191941 ggcagtaatc aatcagaagt taaatttgat ggagtagatt atcgcatcct ggtaaaaatc
   192001 ttcgcccagt ttgtcatccg catatcgata ctgaatcccc gtagtaatgt gtgatgttgc
   192061 attccaccac agtgcaactg cgccattcag cccttcttta ccgccattac ccgctgcata
   192121 ggtagcgtca cggtcaaact cgtactcgtt ccagttggta agagtgaatt tctcgctgcc
   192181 cagcataaag ttgtaacctg caacccagcc ggcgacatag ccgttgtcgc cagtgtaata
   192241 ggtttgatct gtataacgct tagcaaagaa cggtttgaac caccaaccgc tgccggtgaa
   192301 attgtagccg ataccgtaca ggaacatatc gtcgtggaaa ttcacgcgat tcgcagaacc
   192361 gtaggtgcca tacgcatgca agtagagatt aaatccggtg tcacccaggt aaatacggtt
   192421 ggtattttta aaggtataac gctgctcgct acctggttta ttatggcgac cgttatagaa
   192481 gttttcccag tcgaagaacc cgtacatttc tccccaacta aagttagcgc caccttcaaa
   192541 ttcaagataa ccaaaatcat ctttgtgtga tttggtcgac gatttttcgg tagtccggct
   192601 ggtccagtcc agataatgca cgccgatatc tgcaaaaccg cctttaaatt gtgcatgaga
   192661 tacaacaggt atcgctaata tagaggaaag tgtcagagtt aaaagatgtt ttttcatagc
   192721 ataattccct atgccgatcg ttattagtag attgcttaat aaagtggttt gctaaaaacg
   192781 acatattgtt aatgcaaagg aattaatatc gccaatacag tttttaccgt ggattaatag
   192841 agaaaagata taaatccatc gatatcgaat cgctattgaa tgcgtgagaa aagtgtattg
   192901 gtaagagccg ggacaacctc gctataaaaa ggcgttagat tccaccctac agaaaaagga
   192961 tatgtatgtc tcaggttcaa ttttctcata acccgctgtt ttgcatagat attatcaaga
   193021 cttataaacc tgatttcacg ccacgagtgg cctttatttt aggttccggg ctcggcgcgc
   193081 tggccgatca gattgagaac gctgtcgcaa tttcctacga aaagctgccg ggcttcccgg
   193141 taagtacggt acatggtcat gcgggggagt tagtgctggg ccatctccag ggcgtaccgg
   193201 tggtatgtat gaaaggtcgc ggacatttct acgaaggtcg tggaatgacc attatgactg
   193261 acgcaattcg tacctttaag ctgctgggct gcgagctact gttctgcacc aatgcggcag
   193321 gctcgctgcg tccggaagtg ggggcaggca gcctggtcgc attgaaagat catatcaaca
   193381 ccatgcccgg tacgccgatg gtgggtctta acgatgatcg ttttggagag cgcttcttct
   193441 cactggcaaa tgcctacgat gcggaatacc gcgcactgtt acaaaaagtg gcgaaagaag
   193501 agggtttccc tctgacggag ggcgtgttcg tctcgtatcc ggggccgaat ttcgagactg
   193561 cggcggaaat tcgcatgatg caaattattg gtggggatgt tgttggtatg tctgtggtgc
   193621 ctgaggttat ttcagctcgc cattgcgacc ttaaagtcgt tgcggtctct gcgattacca
   193681 atatggcgga aggtttaagc gatgtgaaat tgtcacatgc gcaaacgctg gcggctgcgg
   193741 agctctctaa gcagaacttc atcaacctta tttgtggctt tctgcgcaaa attgcctgag
   193801 aaaaacaata agaaacggcc tcgcgggtga ggccaaatga tgcgacaagg aaacgattat
   193861 gagcatcgcg atgcgcttaa aggtaatgtc ctttttgcaa tattttatct gggggagctg
   193921 gctggttacc ctcggctctt acatgattaa tacccttcat ttcaccggcg ctaatgttgg
   193981 catggtttac agttccaaag ggatcgccgc gattattatg cctggtataa tggggatcat
   194041 cgcagacaaa tggctgcgcg cagaacgtgc atacatgctg tgtcacctgg tgtgtgcggg
   194101 cgtacttttt tatgcggcat ccgtaactga tccggatatg atgttttggg tgatgttagt
   194161 caatgcgatg gcgtttatgc cgactattgc gttatcgaac agcgtctctt attcctgtct
   194221 tgcccaggca gggcttgacc cggtgaccgc tttcccgccc attcgcgttt ttggtacggt
   194281 ggggttcatt gtcgcgatgt gggcagtaag cctgctgcat ctggaattga gtagtctgca
   194341 gctgtatatc gcgtccggtg cgtcattgct gctgtcggct tatgcgctga ctttgccgaa
   194401 gattccggtt gcggagaaaa aagcgaccac atcgcttgcc agcaagctgg gtctggatgc
   194461 cttcgtgctg tttaaaaatc cacgcatggc catctttttc ctctttgcca tgatgctggg
   194521 tgcggtactg caaattacca acgtttttgg taatccgttc ctacatgatt tcgcccgtaa
   194581 cccggagttt gctgacagtt ttgtggtgaa atatccctcc attttactgt cagtttcaca
   194641 gatggcagaa gtgggcttta tactgactat cccattcttt ttaaagcgat ttggcattaa
   194701 a
//
LOCUS       DAFATA010000002       175513 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFATA010000002 DAFATA010000000
VERSION     DAFATA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 175513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 175513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..175513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.002"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..529
                     /locus_tag="LGJ20_000181"
     CDS             <1..529
                     /locus_tag="LGJ20_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBK2384313.1"
                     /translation="KAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDEL
                     IDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHV
                     ENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRNAAM
                     DRNYLASVLAESGLS"
     gene            complement(571..1341)
                     /gene="yafV"
                     /locus_tag="LGJ20_000182"
     CDS             complement(571..1341)
                     /gene="yafV"
                     /locus_tag="LGJ20_000182"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBK2384314.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRLR"
     gene            1522..1968
                     /gene="ivy"
                     /locus_tag="LGJ20_000183"
     CDS             1522..1968
                     /gene="ivy"
                     /locus_tag="LGJ20_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBK2384315.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2011..4455)
                     /gene="fadE"
                     /locus_tag="LGJ20_000184"
     CDS             complement(2011..4455)
                     /gene="fadE"
                     /locus_tag="LGJ20_000184"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBK2384316.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4695..5273
                     /gene="lpcA"
                     /locus_tag="LGJ20_000185"
     CDS             4695..5273
                     /gene="lpcA"
                     /locus_tag="LGJ20_000185"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBK2384317.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5479..6246
                     /locus_tag="LGJ20_000186"
     CDS             5479..6246
                     /locus_tag="LGJ20_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBK2384318.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6217..6957)
                     /locus_tag="LGJ20_000187"
     CDS             complement(6217..6957)
                     /locus_tag="LGJ20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBK2384319.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7113..7391)
                     /gene="yafQ"
                     /locus_tag="LGJ20_000188"
     CDS             complement(7113..7391)
                     /gene="yafQ"
                     /locus_tag="LGJ20_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HBK2384320.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7394..7654)
                     /gene="dinJ"
                     /locus_tag="LGJ20_000189"
     CDS             complement(7394..7654)
                     /gene="dinJ"
                     /locus_tag="LGJ20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBK2384321.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7840..8613
                     /locus_tag="LGJ20_000190"
     CDS             7840..8613
                     /locus_tag="LGJ20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK2384322.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(8670..9026)
                     /locus_tag="LGJ20_000191"
     CDS             complement(8670..9026)
                     /locus_tag="LGJ20_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK2384323.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAHM"
     gene            complement(9019..9297)
                     /locus_tag="LGJ20_000192"
     CDS             complement(9019..9297)
                     /locus_tag="LGJ20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBK2384324.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(9402..11114)
                     /locus_tag="LGJ20_000193"
                     /pseudo
     CDS             complement(9402..11114)
                     /locus_tag="LGJ20_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11086..11871
                     /gene="lafU"
                     /locus_tag="LGJ20_000194"
     CDS             11086..11871
                     /gene="lafU"
                     /locus_tag="LGJ20_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBK2384325.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTVLLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKRLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            11942..12997
                     /gene="dinB"
                     /locus_tag="LGJ20_000195"
     CDS             11942..12997
                     /gene="dinB"
                     /locus_tag="LGJ20_000195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK2384326.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12994..13446
                     /locus_tag="LGJ20_000196"
     CDS             12994..13446
                     /locus_tag="LGJ20_000196"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2384327.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            13692..14831
                     /locus_tag="LGJ20_000197"
     CDS             13692..14831
                     /locus_tag="LGJ20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBK2384328.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            14828..15442
                     /locus_tag="LGJ20_000198"
     CDS             14828..15442
                     /locus_tag="LGJ20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBK2384329.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(15499..16956)
                     /gene="pepD"
                     /locus_tag="LGJ20_000199"
     CDS             complement(15499..16956)
                     /gene="pepD"
                     /locus_tag="LGJ20_000199"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBK2384330.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            17217..17675
                     /gene="gpt"
                     /locus_tag="LGJ20_000200"
     CDS             17217..17675
                     /gene="gpt"
                     /locus_tag="LGJ20_000200"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK2384331.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            17767..19011
                     /gene="frsA"
                     /locus_tag="LGJ20_000201"
     CDS             17767..19011
                     /gene="frsA"
                     /locus_tag="LGJ20_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBK2384332.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            19069..19470
                     /gene="crl"
                     /locus_tag="LGJ20_000202"
     CDS             19069..19470
                     /gene="crl"
                     /locus_tag="LGJ20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBK2384333.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(19509..20564)
                     /gene="phoE"
                     /locus_tag="LGJ20_000203"
     CDS             complement(19509..20564)
                     /gene="phoE"
                     /locus_tag="LGJ20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBK2384334.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            20852..21955
                     /gene="proB"
                     /locus_tag="LGJ20_000204"
     CDS             20852..21955
                     /gene="proB"
                     /locus_tag="LGJ20_000204"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK2384335.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            21967..23220
                     /gene="proA"
                     /locus_tag="LGJ20_000205"
     CDS             21967..23220
                     /gene="proA"
                     /locus_tag="LGJ20_000205"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK2384336.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            23335..23410
                     /locus_tag="LGJ20_000206"
     tRNA            23335..23410
                     /locus_tag="LGJ20_000206"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23368..23370,aa:Thr,seq:cgt)
     gene            complement(23537..23875)
                     /locus_tag="LGJ20_000207"
                     /pseudo
     CDS             complement(23537..23875)
                     /locus_tag="LGJ20_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(23926..24882)
                     /gene="paoD"
                     /locus_tag="LGJ20_000208"
     CDS             complement(23926..24882)
                     /gene="paoD"
                     /locus_tag="LGJ20_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HBK2384337.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHQEEDSCLPPSS"
     gene            complement(24892..27090)
                     /gene="paoC"
                     /locus_tag="LGJ20_000209"
     CDS             complement(24892..27090)
                     /gene="paoC"
                     /locus_tag="LGJ20_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HBK2384338.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            complement(27087..28043)
                     /gene="paoB"
                     /locus_tag="LGJ20_000210"
     CDS             complement(27087..28043)
                     /gene="paoB"
                     /locus_tag="LGJ20_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HBK2384339.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(28040..28729)
                     /gene="paoA"
                     /locus_tag="LGJ20_000211"
     CDS             complement(28040..28729)
                     /gene="paoA"
                     /locus_tag="LGJ20_000211"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HBK2384340.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            29147..29761
                     /locus_tag="LGJ20_000212"
     CDS             29147..29761
                     /locus_tag="LGJ20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HBK2384341.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(30009..30338)
                     /locus_tag="LGJ20_000213"
     CDS             complement(30009..30338)
                     /locus_tag="LGJ20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBK2384342.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(30651..31361)
                     /gene="ecpE"
                     /locus_tag="LGJ20_000214"
     CDS             complement(30651..31361)
                     /gene="ecpE"
                     /locus_tag="LGJ20_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HBK2384343.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            complement(31330..32973)
                     /gene="ecpD"
                     /locus_tag="LGJ20_000215"
     CDS             complement(31330..32973)
                     /gene="ecpD"
                     /locus_tag="LGJ20_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HBK2384344.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            complement(32963..35488)
                     /gene="ecpC"
                     /locus_tag="LGJ20_000216"
                     /gene_synonym="yagX"
     CDS             complement(32963..35488)
                     /gene="ecpC"
                     /locus_tag="LGJ20_000216"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HBK2384345.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            complement(35514..36182)
                     /gene="ecpB"
                     /locus_tag="LGJ20_000217"
                     /gene_synonym="yagY"
     CDS             complement(35514..36182)
                     /gene="ecpB"
                     /locus_tag="LGJ20_000217"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HBK2384346.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            complement(36240..36827)
                     /gene="ecpA"
                     /locus_tag="LGJ20_000218"
                     /gene_synonym="matB"
     CDS             complement(36240..36827)
                     /gene="ecpA"
                     /locus_tag="LGJ20_000218"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HBK2384347.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            complement(36902..37444)
                     /gene="ecpR"
                     /locus_tag="LGJ20_000219"
                     /gene_synonym="matA"
     CDS             complement(36902..37444)
                     /gene="ecpR"
                     /locus_tag="LGJ20_000219"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HBK2384348.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            38267..38494
                     /locus_tag="LGJ20_000220"
     CDS             38267..38494
                     /locus_tag="LGJ20_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384349.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(38529..38669)
                     /gene="ykgO"
                     /locus_tag="LGJ20_000221"
     CDS             complement(38529..38669)
                     /gene="ykgO"
                     /locus_tag="LGJ20_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HBK2384350.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(38669..38932)
                     /locus_tag="LGJ20_000222"
     CDS             complement(38669..38932)
                     /locus_tag="LGJ20_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK2384351.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            complement(39295..39396)
                     /locus_tag="LGJ20_000223"
     CDS             complement(39295..39396)
                     /locus_tag="LGJ20_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384352.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            40512..44765
                     /gene="fdeC"
                     /locus_tag="LGJ20_000224"
     CDS             40512..44765
                     /gene="fdeC"
                     /locus_tag="LGJ20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HBK2384353.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGSWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISNIE
                     VIGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTIVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLAVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKIIRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMK"
     gene            complement(44887..45744)
                     /locus_tag="LGJ20_000225"
     CDS             complement(44887..45744)
                     /locus_tag="LGJ20_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2384354.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMAKEWTINRG
                     LYASFRYEGEWERYPEWSRNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            45993..46862
                     /locus_tag="LGJ20_000226"
     CDS             45993..46862
                     /locus_tag="LGJ20_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK2384355.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(47022..47615)
                     /gene="rclC"
                     /locus_tag="LGJ20_000227"
     CDS             complement(47022..47615)
                     /gene="rclC"
                     /locus_tag="LGJ20_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBK2384356.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(47627..47863)
                     /locus_tag="LGJ20_000228"
     CDS             complement(47627..47863)
                     /locus_tag="LGJ20_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2384357.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(47972..49297)
                     /locus_tag="LGJ20_000229"
     CDS             complement(47972..49297)
                     /locus_tag="LGJ20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBK2384358.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            49523..50377
                     /gene="rclR"
                     /locus_tag="LGJ20_000230"
     CDS             49523..50377
                     /gene="rclR"
                     /locus_tag="LGJ20_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBK2384359.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            50906..51625
                     /locus_tag="LGJ20_000231"
     CDS             50906..51625
                     /locus_tag="LGJ20_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBK2384360.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            51636..53063
                     /locus_tag="LGJ20_000232"
     CDS             51636..53063
                     /locus_tag="LGJ20_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBK2384361.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHELLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHLAQEKKNG"
     gene            53056..53751
                     /locus_tag="LGJ20_000233"
     CDS             53056..53751
                     /locus_tag="LGJ20_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBK2384362.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(53997..54662)
                     /locus_tag="LGJ20_000234"
     CDS             complement(53997..54662)
                     /locus_tag="LGJ20_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384363.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(54846..56039)
                     /locus_tag="LGJ20_000235"
     CDS             complement(54846..56039)
                     /locus_tag="LGJ20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2384364.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSL
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            complement(56061..57143)
                     /locus_tag="LGJ20_000236"
                     /pseudo
     CDS             complement(56061..57143)
                     /locus_tag="LGJ20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(57185..57946)
                     /gene="hyxA"
                     /locus_tag="LGJ20_000237"
     CDS             complement(57185..57946)
                     /gene="hyxA"
                     /locus_tag="LGJ20_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HBK2384365.1"
                     /translation="MRFVLFCPSGIVPAQFEALSTGSVCNITCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKTGMS
                     VERAISVANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            complement(58100..58894)
                     /gene="hyxR"
                     /locus_tag="LGJ20_000238"
     CDS             complement(58100..58894)
                     /gene="hyxR"
                     /locus_tag="LGJ20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HBK2384366.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPASSLYNGFP
                     HIGTLLKASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATQALRKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVIVGKSINSDAISGKVLSGQRKHMSKKRPGVL"
     gene            complement(59224..59787)
                     /gene="ipbA"
                     /locus_tag="LGJ20_000239"
                     /gene_synonym="fimX"
     CDS             complement(59224..59787)
                     /gene="ipbA"
                     /locus_tag="LGJ20_000239"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HBK2384367.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
     gene            60033..60166
                     /locus_tag="LGJ20_000240"
                     /pseudo
     CDS             60033..60166
                     /locus_tag="LGJ20_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60862..62532)
                     /gene="betA"
                     /locus_tag="LGJ20_000241"
     CDS             complement(60862..62532)
                     /gene="betA"
                     /locus_tag="LGJ20_000241"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBK2384368.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(62546..64018)
                     /gene="betB"
                     /locus_tag="LGJ20_000242"
     CDS             complement(62546..64018)
                     /gene="betB"
                     /locus_tag="LGJ20_000242"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBK2384369.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(64032..64619)
                     /gene="betI"
                     /locus_tag="LGJ20_000243"
     CDS             complement(64032..64619)
                     /gene="betI"
                     /locus_tag="LGJ20_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBK2384370.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            64748..66781
                     /gene="betT"
                     /locus_tag="LGJ20_000244"
     CDS             64748..66781
                     /gene="betT"
                     /locus_tag="LGJ20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBK2384371.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            67356..71339
                     /gene="ehaA"
                     /locus_tag="LGJ20_000245"
     CDS             67356..71339
                     /gene="ehaA"
                     /locus_tag="LGJ20_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HBK2384372.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVTIEKNGAL
                     GITQNIGSAEITNRGKLHLKAEDSMTFANKISGNGTISIDSGTVELTGNNYAFYGYID
                     VASDAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALAAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKISGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGIGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGRNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            71481..72569
                     /locus_tag="LGJ20_000246"
     CDS             71481..72569
                     /locus_tag="LGJ20_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2384373.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(72611..73543)
                     /locus_tag="LGJ20_000247"
     CDS             complement(72611..73543)
                     /locus_tag="LGJ20_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2384374.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAADELGLTTSAISYTIKRME
                     TGLDVVLFTRNTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFMPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(73635..74132)
                     /locus_tag="LGJ20_000248"
     CDS             complement(73635..74132)
                     /locus_tag="LGJ20_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBK2384375.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            74390..74995
                     /locus_tag="LGJ20_000249"
     CDS             74390..74995
                     /locus_tag="LGJ20_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBK2384376.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            75035..75898
                     /locus_tag="LGJ20_000250"
     CDS             75035..75898
                     /locus_tag="LGJ20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBK2384377.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYRGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHHFIHDIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            75888..77435
                     /gene="fdrA"
                     /locus_tag="LGJ20_000251"
     CDS             75888..77435
                     /gene="fdrA"
                     /locus_tag="LGJ20_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBK2384378.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            77435..78853
                     /locus_tag="LGJ20_000252"
     CDS             77435..78853
                     /locus_tag="LGJ20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBK2384379.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            78996..79946
                     /locus_tag="LGJ20_000253"
     CDS             78996..79946
                     /locus_tag="LGJ20_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBK2384380.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            79956..81338
                     /locus_tag="LGJ20_000254"
     CDS             79956..81338
                     /locus_tag="LGJ20_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK2384381.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            81637..82047
                     /locus_tag="LGJ20_000255"
     CDS             81637..82047
                     /locus_tag="LGJ20_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK2384382.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            82301..83284
                     /locus_tag="LGJ20_000256"
     CDS             82301..83284
                     /locus_tag="LGJ20_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2384383.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            83333..84817
                     /locus_tag="LGJ20_000257"
     CDS             83333..84817
                     /locus_tag="LGJ20_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK2384384.1"
                     /translation="METFLSLRHINKKFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRLDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            84810..85781
                     /locus_tag="LGJ20_000258"
     CDS             84810..85781
                     /locus_tag="LGJ20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2384385.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            85778..86734
                     /locus_tag="LGJ20_000259"
     CDS             85778..86734
                     /locus_tag="LGJ20_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2384386.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            86821..87870
                     /gene="yahK"
                     /locus_tag="LGJ20_000260"
     CDS             86821..87870
                     /gene="yahK"
                     /locus_tag="LGJ20_000260"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBK2384387.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMWIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            88113..88928
                     /locus_tag="LGJ20_000261"
     CDS             88113..88928
                     /locus_tag="LGJ20_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384388.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            89341..89586
                     /locus_tag="LGJ20_000262"
     CDS             89341..89586
                     /locus_tag="LGJ20_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384389.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(89603..90274)
                     /locus_tag="LGJ20_000263"
     CDS             complement(89603..90274)
                     /locus_tag="LGJ20_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBK2384390.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            90421..90696
                     /locus_tag="LGJ20_000264"
     CDS             90421..90696
                     /locus_tag="LGJ20_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2384391.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(90797..92383)
                     /gene="prpR"
                     /locus_tag="LGJ20_000265"
     CDS             complement(90797..92383)
                     /gene="prpR"
                     /locus_tag="LGJ20_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HBK2384392.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            92622..93512
                     /gene="prpB"
                     /locus_tag="LGJ20_000266"
     CDS             92622..93512
                     /gene="prpB"
                     /locus_tag="LGJ20_000266"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HBK2384393.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            93557..94726
                     /gene="prpC"
                     /locus_tag="LGJ20_000267"
     CDS             93557..94726
                     /gene="prpC"
                     /locus_tag="LGJ20_000267"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBK2384394.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            94760..96211
                     /gene="prpD"
                     /locus_tag="LGJ20_000268"
     CDS             94760..96211
                     /gene="prpD"
                     /locus_tag="LGJ20_000268"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HBK2384395.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            96251..98137
                     /gene="prpE"
                     /locus_tag="LGJ20_000269"
     CDS             96251..98137
                     /gene="prpE"
                     /locus_tag="LGJ20_000269"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HBK2384396.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            98374..99633
                     /gene="codB"
                     /locus_tag="LGJ20_000270"
     CDS             98374..99633
                     /gene="codB"
                     /locus_tag="LGJ20_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBK2384397.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            99623..100906
                     /gene="codA"
                     /locus_tag="LGJ20_000271"
     CDS             99623..100906
                     /gene="codA"
                     /locus_tag="LGJ20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HBK2384398.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(100946..101845)
                     /gene="cynR"
                     /locus_tag="LGJ20_000272"
     CDS             complement(100946..101845)
                     /gene="cynR"
                     /locus_tag="LGJ20_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HBK2384399.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            101955..102614
                     /gene="cynT"
                     /locus_tag="LGJ20_000273"
     CDS             101955..102614
                     /gene="cynT"
                     /locus_tag="LGJ20_000273"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HBK2384400.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            102645..103115
                     /gene="cynS"
                     /locus_tag="LGJ20_000274"
     CDS             102645..103115
                     /gene="cynS"
                     /locus_tag="LGJ20_000274"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HBK2384401.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            103121..104302
                     /gene="cynX"
                     /locus_tag="LGJ20_000275"
     CDS             103121..104302
                     /gene="cynX"
                     /locus_tag="LGJ20_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HBK2384402.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPQVMGLWSAALMGGGGLGAAITPWLVQHSE
                     SWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(104405..105016)
                     /gene="lacA"
                     /locus_tag="LGJ20_000276"
     CDS             complement(104405..105016)
                     /gene="lacA"
                     /locus_tag="LGJ20_000276"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HBK2384403.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFKDYKVESSV"
     gene            complement(105082..106335)
                     /gene="lacY"
                     /locus_tag="LGJ20_000277"
     CDS             complement(105082..106335)
                     /gene="lacY"
                     /locus_tag="LGJ20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HBK2384404.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(106387..109461)
                     /gene="lacZ"
                     /locus_tag="LGJ20_000278"
     CDS             complement(106387..109461)
                     /gene="lacZ"
                     /locus_tag="LGJ20_000278"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBK2384405.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKVAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(109584..110666)
                     /gene="lacI"
                     /locus_tag="LGJ20_000279"
     CDS             complement(109584..110666)
                     /gene="lacI"
                     /locus_tag="LGJ20_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBK2384406.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(110743..111690)
                     /gene="mhpR"
                     /locus_tag="LGJ20_000280"
     CDS             complement(110743..111690)
                     /gene="mhpR"
                     /locus_tag="LGJ20_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HBK2384407.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            111767..113431
                     /locus_tag="LGJ20_000281"
     CDS             111767..113431
                     /locus_tag="LGJ20_000281"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBK2384408.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            113433..114377
                     /gene="mhpB"
                     /locus_tag="LGJ20_000282"
     CDS             113433..114377
                     /gene="mhpB"
                     /locus_tag="LGJ20_000282"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBK2384409.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            114395..115261
                     /gene="mhpC"
                     /locus_tag="LGJ20_000283"
     CDS             114395..115261
                     /gene="mhpC"
                     /locus_tag="LGJ20_000283"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBK2384410.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            115271..116080
                     /gene="mhpD"
                     /locus_tag="LGJ20_000284"
     CDS             115271..116080
                     /gene="mhpD"
                     /locus_tag="LGJ20_000284"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBK2384411.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            116077..117027
                     /gene="mhpF"
                     /locus_tag="LGJ20_000285"
     CDS             116077..117027
                     /gene="mhpF"
                     /locus_tag="LGJ20_000285"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBK2384412.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            117024..118037
                     /gene="dmpG"
                     /locus_tag="LGJ20_000286"
     CDS             117024..118037
                     /gene="dmpG"
                     /locus_tag="LGJ20_000286"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBK2384413.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            118213..119424
                     /gene="mhpT"
                     /locus_tag="LGJ20_000287"
     CDS             118213..119424
                     /gene="mhpT"
                     /locus_tag="LGJ20_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBK2384414.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            119526..120065
                     /locus_tag="LGJ20_000288"
     CDS             119526..120065
                     /locus_tag="LGJ20_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBK2384415.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(120190..121023)
                     /gene="fghA"
                     /locus_tag="LGJ20_000289"
     CDS             complement(120190..121023)
                     /gene="fghA"
                     /locus_tag="LGJ20_000289"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBK2384416.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYNYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(121116..122225)
                     /gene="frmA"
                     /locus_tag="LGJ20_000290"
     CDS             complement(121116..122225)
                     /gene="frmA"
                     /locus_tag="LGJ20_000290"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBK2384417.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(122260..122535)
                     /gene="frmR"
                     /locus_tag="LGJ20_000291"
     CDS             complement(122260..122535)
                     /gene="frmR"
                     /locus_tag="LGJ20_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBK2384418.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(122720..123493)
                     /locus_tag="LGJ20_000292"
     CDS             complement(122720..123493)
                     /locus_tag="LGJ20_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBK2384419.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETENFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(123495..124076)
                     /locus_tag="LGJ20_000293"
     CDS             complement(123495..124076)
                     /locus_tag="LGJ20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK2384420.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            complement(124054..125250)
                     /locus_tag="LGJ20_000294"
     CDS             complement(124054..125250)
                     /locus_tag="LGJ20_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK2384421.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(125260..125931)
                     /locus_tag="LGJ20_000295"
     CDS             complement(125260..125931)
                     /locus_tag="LGJ20_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBK2384422.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            126547..127509
                     /gene="tauA"
                     /locus_tag="LGJ20_000296"
     CDS             126547..127509
                     /gene="tauA"
                     /locus_tag="LGJ20_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2384423.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            127522..128289
                     /gene="tauB"
                     /locus_tag="LGJ20_000297"
     CDS             127522..128289
                     /gene="tauB"
                     /locus_tag="LGJ20_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBK2384424.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            128286..129113
                     /gene="tauC"
                     /locus_tag="LGJ20_000298"
     CDS             128286..129113
                     /gene="tauC"
                     /locus_tag="LGJ20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBK2384425.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            129110..129961
                     /gene="tauD"
                     /locus_tag="LGJ20_000299"
     CDS             129110..129961
                     /gene="tauD"
                     /locus_tag="LGJ20_000299"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBK2384426.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(130067..131041)
                     /gene="hemB"
                     /locus_tag="LGJ20_000300"
     CDS             complement(130067..131041)
                     /gene="hemB"
                     /locus_tag="LGJ20_000300"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBK2384427.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            131566..134472
                     /locus_tag="LGJ20_000301"
     CDS             131566..134472
                     /locus_tag="LGJ20_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2384428.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWTYIDNPVTALD
                     WDHMDKAGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYADSTAN
                     QLDISNSVIHGSITSMLPGGYYDRFDADGNNLGGYDFYTDAVVDTHWRDGDVFTLNIA
                     NTTIDDDYEALYFTDSYKDGDVTKHTNETFDTSEGVAVNLDVESNINISNNSRVAGIA
                     LSQGNTYNETYTTESHTWDNNISVKDSTVTSGSNYILDSNTYGKTGHFGNSDEPSDYA
                     GPGDVAMSFTASGSDYAMKNNVFLSNSTLMGDVAFTSTWNSNFDPNGHDSNGDGVKDT
                     NGGWTDDSLNVDELNLTLDNGSKWVGQAIYNVAETSAMYDVATNSLTPDATYENNDWK
                     RVVDDKVFQSGVFNVALNNGSEWDTTGRSIVDTLTVNNGSQVNVSESKLTSDTIDLTN
                     GSSLNIGEDGYVDTDHLTINSYSTVALTESTGWGADYNLYANTITVTNGGVLDVNVDQ
                     FDTEAFRTDKLELTSGNIADHNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNYGY
                     GIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKD
                     KEIIYVNDVNTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSAN
                     TNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVD
                     TKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYS
                     HFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDD
                     YQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDLNG
                     DSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            134560..135183
                     /gene="iprA"
                     /locus_tag="LGJ20_000302"
     CDS             134560..135183
                     /gene="iprA"
                     /locus_tag="LGJ20_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBK2384429.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(135184..136341)
                     /gene="ampH"
                     /locus_tag="LGJ20_000303"
     CDS             complement(135184..136341)
                     /gene="ampH"
                     /locus_tag="LGJ20_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBK2384430.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            136693..137913
                     /gene="sbmA"
                     /locus_tag="LGJ20_000304"
     CDS             136693..137913
                     /gene="sbmA"
                     /locus_tag="LGJ20_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBK2384431.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLL
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            137926..139020
                     /gene="yaiW"
                     /locus_tag="LGJ20_000305"
     CDS             137926..139020
                     /gene="yaiW"
                     /locus_tag="LGJ20_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBK2384432.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASSVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(139079..139387)
                     /locus_tag="LGJ20_000306"
     CDS             complement(139079..139387)
                     /locus_tag="LGJ20_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBK2384433.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            139647..139859
                     /locus_tag="LGJ20_000307"
     CDS             139647..139859
                     /locus_tag="LGJ20_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBK2384434.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(139883..140977)
                     /gene="ddlA"
                     /locus_tag="LGJ20_000308"
     CDS             complement(139883..140977)
                     /gene="ddlA"
                     /locus_tag="LGJ20_000308"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK2384435.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLLTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            141055..141258
                     /locus_tag="LGJ20_000309"
     CDS             141055..141258
                     /locus_tag="LGJ20_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384436.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            141440..141700
                     /gene="iraP"
                     /locus_tag="LGJ20_000310"
     CDS             141440..141700
                     /gene="iraP"
                     /locus_tag="LGJ20_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBK2384437.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            141801..143216
                     /gene="phoA"
                     /locus_tag="LGJ20_000311"
     CDS             141801..143216
                     /gene="phoA"
                     /locus_tag="LGJ20_000311"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBK2384438.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAIQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            143335..143655
                     /gene="psiF"
                     /locus_tag="LGJ20_000312"
     CDS             143335..143655
                     /gene="psiF"
                     /locus_tag="LGJ20_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBK2384439.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            143757..144872
                     /gene="adrA"
                     /locus_tag="LGJ20_000313"
                     /gene_synonym="dgcC"
     CDS             143757..144872
                     /gene="adrA"
                     /locus_tag="LGJ20_000313"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBK2384440.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(144889..145698)
                     /gene="proC"
                     /locus_tag="LGJ20_000314"
     CDS             complement(144889..145698)
                     /gene="proC"
                     /locus_tag="LGJ20_000314"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK2384441.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            145818..146276
                     /locus_tag="LGJ20_000315"
     CDS             145818..146276
                     /locus_tag="LGJ20_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK2384442.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            146459..146983
                     /gene="aroL"
                     /locus_tag="LGJ20_000316"
     CDS             146459..146983
                     /gene="aroL"
                     /locus_tag="LGJ20_000316"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBK2384443.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            147033..147224
                     /gene="yaiA"
                     /locus_tag="LGJ20_000317"
     CDS             147033..147224
                     /gene="yaiA"
                     /locus_tag="LGJ20_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBK2384444.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            147482..148159
                     /locus_tag="LGJ20_000318"
     CDS             147482..148159
                     /locus_tag="LGJ20_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBK2384445.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            148231..148515
                     /gene="ppnP"
                     /locus_tag="LGJ20_000319"
     CDS             148231..148515
                     /gene="ppnP"
                     /locus_tag="LGJ20_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBK2384446.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            148719..149138
                     /locus_tag="LGJ20_000320"
     CDS             148719..149138
                     /locus_tag="LGJ20_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384447.1"
                     /translation="MYELLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(149216..150127)
                     /gene="rdgC"
                     /locus_tag="LGJ20_000321"
     CDS             complement(149216..150127)
                     /gene="rdgC"
                     /locus_tag="LGJ20_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBK2384448.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            150252..151160
                     /gene="mak"
                     /locus_tag="LGJ20_000322"
     CDS             150252..151160
                     /gene="mak"
                     /locus_tag="LGJ20_000322"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBK2384449.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(151303..152487)
                     /gene="araJ"
                     /locus_tag="LGJ20_000323"
     CDS             complement(151303..152487)
                     /gene="araJ"
                     /locus_tag="LGJ20_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBK2384450.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(152613..155756)
                     /gene="sbcC"
                     /locus_tag="LGJ20_000324"
     CDS             complement(152613..155756)
                     /gene="sbcC"
                     /locus_tag="LGJ20_000324"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBK2384451.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFSREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(155753..156955)
                     /gene="sbcD"
                     /locus_tag="LGJ20_000325"
     CDS             complement(155753..156955)
                     /gene="sbcD"
                     /locus_tag="LGJ20_000325"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBK2384452.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            157145..157834
                     /gene="phoB"
                     /locus_tag="LGJ20_000326"
     CDS             157145..157834
                     /gene="phoB"
                     /locus_tag="LGJ20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBK2384453.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            157892..159187
                     /gene="phoR"
                     /locus_tag="LGJ20_000327"
     CDS             157892..159187
                     /gene="phoR"
                     /locus_tag="LGJ20_000327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBK2384454.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            159594..160913
                     /gene="brnQ"
                     /locus_tag="LGJ20_000328"
     CDS             159594..160913
                     /gene="brnQ"
                     /locus_tag="LGJ20_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBK2384455.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            160989..162362
                     /gene="proY"
                     /locus_tag="LGJ20_000329"
     CDS             160989..162362
                     /gene="proY"
                     /locus_tag="LGJ20_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBK2384456.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            162518..164335
                     /gene="malZ"
                     /locus_tag="LGJ20_000330"
     CDS             162518..164335
                     /gene="malZ"
                     /locus_tag="LGJ20_000330"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBK2384457.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(164340..164921)
                     /gene="acpH"
                     /locus_tag="LGJ20_000331"
     CDS             complement(164340..164921)
                     /gene="acpH"
                     /locus_tag="LGJ20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBK2384458.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            165014..166084
                     /gene="queA"
                     /locus_tag="LGJ20_000332"
     CDS             165014..166084
                     /gene="queA"
                     /locus_tag="LGJ20_000332"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK2384459.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            166140..167267
                     /gene="tgt"
                     /locus_tag="LGJ20_000333"
     CDS             166140..167267
                     /gene="tgt"
                     /locus_tag="LGJ20_000333"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK2384460.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            167290..167622
                     /gene="yajC"
                     /locus_tag="LGJ20_000334"
     CDS             167290..167622
                     /gene="yajC"
                     /locus_tag="LGJ20_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK2384461.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            167650..169497
                     /gene="secD"
                     /locus_tag="LGJ20_000335"
     CDS             167650..169497
                     /gene="secD"
                     /locus_tag="LGJ20_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBK2384462.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            169508..170479
                     /gene="secF"
                     /locus_tag="LGJ20_000336"
     CDS             169508..170479
                     /gene="secF"
                     /locus_tag="LGJ20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBK2384463.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            170607..170954
                     /locus_tag="LGJ20_000337"
     CDS             170607..170954
                     /locus_tag="LGJ20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBK2384464.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(171131..172015)
                     /gene="tsx"
                     /locus_tag="LGJ20_000338"
     CDS             complement(171131..172015)
                     /gene="tsx"
                     /locus_tag="LGJ20_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBK2384465.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(172314..172853)
                     /locus_tag="LGJ20_000339"
     CDS             complement(172314..172853)
                     /locus_tag="LGJ20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBK2384466.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            173004..173453
                     /gene="nrdR"
                     /locus_tag="LGJ20_000340"
     CDS             173004..173453
                     /gene="nrdR"
                     /locus_tag="LGJ20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBK2384467.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            173457..174560
                     /gene="ribD"
                     /locus_tag="LGJ20_000341"
     CDS             173457..174560
                     /gene="ribD"
                     /locus_tag="LGJ20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBK2384468.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            174649..175119
                     /gene="ribE"
                     /locus_tag="LGJ20_000342"
     CDS             174649..175119
                     /gene="ribE"
                     /locus_tag="LGJ20_000342"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBK2384469.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            175139..>175513
                     /gene="nusB"
                     /locus_tag="LGJ20_000343"
     CDS             175139..>175513
                     /gene="nusB"
                     /locus_tag="LGJ20_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK2384470.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNG"
BASE COUNT        42540 a        43211 c        46109 g        43653 t
ORIGIN      
        1 taaagccttt gaggaaaaat ttccgctgaa agaattaaat aatccagagc atgacagtta
       61 cgcaatcagt gaaaagagtc acggcagaga agaaatccgt cttcatattg tttgcgatgt
      121 ccctgatgaa cttattgatt tcacgtttga atggaaaggg ctgaagaaat tatgcgtggc
      181 agtctccttt cggtccataa tagcagaaca aaagaaagag ccagaaatga cggtcagata
      241 ttatatcagt tctgctgatt taaccgcaga gaagttcgcc acagcaatcc gaaaccactg
      301 gcacgtggag aataagctgc actggcgtct ggacgtggta atgaatgaag acgactgcaa
      361 aataagaaga ggaaatgcag cagaattatt ttcagggata cggcacatcg ctattaatat
      421 tttgacgaat gataaggtat tcaaggcagg gttaagacgt aagatgcgaa atgcagctat
      481 ggacagaaac tatctggcgt cagtccttgc ggagagcggg ctttcgtagt cttaccccga
      541 ctctccccca gccttaaaca caacccccac tcaccgcaac ctaaactcat ccgcatcccg
      601 ccatgccgga aacttatcgc gatattccct taacaccgcc atcgacagct cggcatcaat
      661 gcgcgttgcc tgatgcgcgt cggcagtagc gataatctcg ccttgtggat taatcacccg
      721 gctgtcaccg cgataatggc agccgttgcc atcgctgccg acgcgattgc atcccgccac
      781 atacgcctga ttctcaatcg cgcgggccgt cagcaatgcc tgccagtgca gagagcgcgg
      841 agcaggccag ttggcgacgt acagggcgag gtcataatcg ttgagattgc gcgaccacac
      901 aggaaaacgt aagtcgtagc acaccagcgg caaaatacgc cagccgcgcc attccacaat
      961 cactcgcgca ttgcccgctt tataatgtag atgctcatct gccatgcgga acagatgacg
     1021 cttatcataa aaatgtaccg tgccgcccgg ctcaaccagc aaaaagcggt taaccgaacc
     1081 agactccgtt tgtaatgcaa cactgcctgc aatcagcgca ttgcactgct gcgccttagc
     1141 tgtcatccag ttcactacgt catcttgtgc tagcgacgaa gctgccgctt ccatggcaaa
     1201 gccgctggta aacatctccg gtagaacgat cacatcgcgc ccggtaatac cttccagttg
     1261 acgatcaaaa tgacgcaggt tggcagggcc atccatccac accagtggtt gctgcaaaag
     1321 cgtaatcttc aaaccaggca cggtgtacaa ctcctttatg cgaagggttt tataacttta
     1381 acaccttatc aggcagttgc cttagcgcag aataaattga taacaaatgc tgatattgga
     1441 aatatctgat ttgcaaatta tcgtgttatc gccaggcttt agaaggttaa taacatgggc
     1501 aggataagct cgggaggaat gatgtttaag gcaataacga cagtcgccgc tctggtcatc
     1561 gccaccagtg caatggcgca ggatgattta accattagca gccttgcaaa gggcgaaacc
     1621 accaaagctg catttaatca gatggtacaa gggcataagc tgcctgcctg ggtgatgaaa
     1681 ggcggtactt atactcccgc acaaaccgta acgttgggag atgagacgta tcaggtgatg
     1741 agcgcgtgca aaccgcatga ctgtggctcg caacgtatcg ctgtgatgtg gtccgagaaa
     1801 tctaatcaga tgacggggct gttctcgact attgatgaga aaacgtcgca agagaaactc
     1861 acctggctga atgtgaacga tgcgctttcg attgatggta aaacggtgct gttcgcggcg
     1921 ttgaccggca gcctggaaaa ccatccggat ggctttaatt ttaaataatt agcggataaa
     1981 gaaacggagc ctttcggctc cgttattcaa ttacgcggct tcaactttcc gcactttctc
     2041 cggcaacttt accggcttcg tcgccagctc ttccggatca aagtcatcaa cgttaatact
     2101 gcgcagacgg ctttcttcag ctttcaccag aatagcggct tcatctttat caatcagccc
     2161 cttcgccagc gcgttgtgcg ccagttcatc cagacgggta aacggcaggt ttttacccag
     2221 ctctttacag atccgctgat gaattgggtc ggcggcaatc acatccacca gcgcctcttc
     2281 cagcaagcca accggattat gctcgctcgg cgtcaggtac tgaccgcgac caatgcggga
     2341 acgggtggcg ttcggcactt gtaaaatctt cgccacttta tgatccagct tgtcagaagg
     2401 tgccagataa tgacgtccgg tcgggaagat caccacattc agcagcccgg caaccacgcg
     2461 gttcgggaag ttttgcagta aatcatccat cgcctgttca gcctgataca gcgcatcttg
     2521 tacgccccag tgcaccagcg gcaggtcggc ttcattacgg ccttcgtcgt cataacgctt
     2581 cagaacggca gaggcgaggt aaagctggct taaaatatcc cccagacgtg ccgagatgcg
     2641 ctcacgacgt ttcaggctgc cgcccagcac agccatcgag acatcagaaa gcagggcgag
     2701 gttggcgctc aggcggttca ggtgctgata gtagcgttta gtggcatcgc cggttggcgt
     2761 gctgctggtt aaaccgcgcg tcaggcccag ccagaagctg cgaactttgt tgctaccgac
     2821 gtgaccgata tgtttgaaca acagtttatc gaacgcgttg acgtcattgt tcttcgctgc
     2881 ttccatctct tccagcacgt acggatggca acgaatcgct ccttgtccga agatcatcat
     2941 gctgcgggtc agaatgttgg ccccttcaac ggtgatggca atcggtgcgc cctggtaagc
     3001 acgcgccagg aagttgctct gcccgagcat aatgcctttg ccaccagtga tatccatcgc
     3061 atcaataatc gactgctgcc cgcggtgggt acagtgatac ttaacgatag ccgacagcac
     3121 ggcaggtttt tcgccgagca taatgccgta ggtaatcagc gatgccgcag catccatcac
     3181 gtaggcatta ccggcaatac gcgccagcgg ctcttcaatc ccttccatct taccgataga
     3241 gattttgaac tgacggcgaa tgtgagcata cgcgcccgtt gccagcgcta ccgatttcac
     3301 gccgccggtt gagttggaag gcagggtgat gccgcggcct accgagaggc actccaccag
     3361 catccgccag ccttgcccgg ccattttcgg ccccccgatg atgtaatcga tcggtacgaa
     3421 gacatcttta ccgcgcgtcg gtccgttctg gaacggtacg ttcagcggga agtggcgacg
     3481 accaatttcc acgcccggcg tggtggtcgg gatcagcgca caggtaatac ctaaatcttc
     3541 tgcgccaccc agcagctttt ccgggtcgga gagtttaaac gccagcccaa gcacagtcgc
     3601 aatcggtgcc agcgtaatat agcgtttgtt ccaggtcaga cgcatcccca gcacctgctg
     3661 gccctgccat tcgcccatgc agacaatccc ggtgtccgga atcgcgcccg catcggaacc
     3721 cgcttccggg cttgtcagtg caaagcaagg gatctcctga ccacgcgcca gacgcggcag
     3781 atagtgatct ttctgctcgt cagtgccgta atgttgcagc agctcgcccg ggcctaatga
     3841 gttcggcacg ccgacggtaa tcgccaggat cccgctcacg ccggagagtt tttgcagcac
     3901 gcgagactgg gcataagctg agaactccag tccgccgtac tcttttttga taatcatcgc
     3961 gaagaagcga tgctctttaa ggtacgccca caactccggc ggcagatccg ccagctcatg
     4021 agtaatctgg aaatcattcg ccatccggca ggcttcttct accgggccgt cgagaaacgc
     4081 ttgctcttcg gcggtcaggc gcggctgcgg atagttatgc agctttttcc agtccggctt
     4141 gccctggaac aagtcgccct cccaccaggt ggtgcccgca tcaatcgctt ctttctcagt
     4201 gcgcgacatc ggcggcatca ccttacggaa accgcgaaat accggcgcgg aaatcatcga
     4261 cttacgcata ggcgcaaagt taaatggcac gaggataatg gccagaggca ccagtaccca
     4321 cgccgaccac agaccagcaa cgccgagggc ggctgtccag gcgagcaaaa tcagactgct
     4381 gataaataag ctcacgcggt gatagaacaa cgcgccgagc aggacaaccg tagcgagaat
     4441 actcaaaatc atcataacga aaagcccctt acttgtagga ggtctgacca cttgtgatga
     4501 tatggttgta gtggatgtaa aaacatttag caatgtgttt acaatataat tacaacaaag
     4561 ctcacattgt tgctgttttt atccgcactt caggtcaaaa agtcctggtc atagcacctg
     4621 cccgtacttc tcgcttttgg cggtatccgg tacactgcat tttgtctatt acatttatgc
     4681 tgaaggatat cctcatgtac caggatctta ttcgtaacga actgaacgaa gcggcggaaa
     4741 cgctggctaa ctttttaaaa gatgacgcca atattcacgc cattcagcgc gcggcggtcc
     4801 tgttagcaga cagctttaaa gccggtggca aagtgctttc ctgcggcaac ggaggttccc
     4861 attgcgacgc tatgcacttt gccgaagagt tgaccggtcg ctaccgtgaa aaccgtccgg
     4921 gctacccggc gattgctatt tctgacgtta gtcatatttc ctgcgtcggt aatgatttcg
     4981 gtttcaatga tattttctcc cgctacgttg aagcggtagg tcgcgaaggc gatgtactgc
     5041 tggggatctc cacctccggt aactctgcaa acgtgatcaa agcgatcgca gcggcgcgtg
     5101 agaaggggat gaaagtgatc accctgaccg gtaaagacgg cggcaaaatg gctggcacgg
     5161 cggatatcga aattcgcgta ccgcactttg gttatgccga ccgcattcag gagattcaca
     5221 ttaaagtgat ccatatcctg atccagttaa ttgaaaaaga gatggttaag taagtctggc
     5281 gtaggctgga taaggcgttt acgccgcatc cggcacttgt gcgctgatgc ctgatgcgat
     5341 gctgacgcgt cttatcatgc ctacaaatct gtacccgaac cgtaggccga ataattcgtt
     5401 tacgccgcat ccgacctgaa aattcttaaa tcaatcttcg ccgggggcca ttcgctcccg
     5461 ctgttgtgga ggttacccat gtgcgaattg ctcgggatga gcgccaacgt ccctaccgat
     5521 atctgcttta gtttcaccgg acttgtacag cgtggtggtg gaaccgggcc acataaagat
     5581 ggctggggca ttacctttta cgaaggtaaa ggctgtcgca catttaaaga tccacaaccc
     5641 agctttaatt cccccatcgc caaacttgtc caggactacc cgataaaatc ctgttcggtg
     5701 gtggctcata ttcgccaggc taatcggggc gaggtggcgc tggaaaatac tcacccattt
     5761 acccgcgagt tatgggggcg taactggact tatgcccata acggacaact gacgggctac
     5821 aaatcactgg aaaccggcaa cttccgcccg gtcggtgaaa ccgatagcga aaaagccttc
     5881 tgctggctcc tgcataaatt aacgcagcgt tacccgcgca caccgggcaa catggcggca
     5941 gtgtttaaat atatcgcctc actggcggat gaactgcggc agaagggcgt tttcaacatg
     6001 ctgctttctg acgggcgcta tgtgatggcg tattgctcga ctaatttaca ctggatcacc
     6061 cgccgcgcgc cgtttggcgt ggcaacgctg ctggatcagg atgtggaaat cgacttcagc
     6121 tcgcagacca caccgaatga tgtggtcacg gtgattgcaa cacagccgct gacgggcaat
     6181 gaaacctggc aaaagattat gccaggcgaa tggcgcttat tttgcctcgg ggagcgtgta
     6241 gtttgatgcc agttgtggct gcacaacttc gtggcttaac ggcttgctga ccacgtaacg
     6301 accattgacc acagaaacgg tcggtggctt acgggtttgc tcaaagtagt cgtaacccgg
     6361 cttcagttgc tcccagaagt ccttaaagtt ggaatattta tggcgcttca tattggcgtc
     6421 ggtcatgcgg aacgggtaaa tactcacctg cacgctcggc tgaccaaaca ccagcgcacc
     6481 agtaacgaac tggaagatct catcaatacc ctgattggtc attgcgtagc agccgatgga
     6541 aacacaatcg ccgtggatca tcaggtattt cccttcataa ccatgcgcac ggtcataggc
     6601 attggggaaa ccaatattaa tcgctttgta gtaacggctg tctggtttta actgattacg
     6661 ctggacgcta taaaaccctt ccgggctttt gaaatcgccc tgacgctgtt ttggccctaa
     6721 gccgccggaa tatttacaga ttttataact gtcgagcagc tgatattgct cgcccatttt
     6781 gacgtagaga tcgagcgtac gttcttcctt gaagatctgg atgtagacag gggatcccat
     6841 caactgctgc ttatactctt tgctcactgg cgtggtcgag ctactgctgc ccagcaaacc
     6901 ggcaaacgaa acgcacggga tcaacaacat cgcaagaatt aatgcgattt tacgcatact
     6961 gcttattcct tgataaaacg gttacacacg ccaggacggc aaaatagatc ccaaatcgga
     7021 atagtctgga tttggaaggc tcacattatc accaaaagag ttttacgcaa gcctgtcggc
     7081 gcagggttta caaatttcat cggcagcgtg ctttacccaa agagcgccgc gtgagttcca
     7141 gttctctcaa atcgtaaaag tttatcggta agtttgtaaa tcaggatcca gtccggttcg
     7201 acatgagcat cgcgataacc tttccatgaa ccttgcagcg ggtggtcttt ataaacagct
     7261 ggaagcggta aagcattatt gataagaagc gtcataagat atttcaattt attcatatcc
     7321 ttatgacgct tttgtgcaag ttttacatcc tttgaaaatt gtcccgagta ttcaatatcc
     7381 ctttgaatca tatttaaatt cctaatttat caaataaatc atcggcgtct ttggccttat
     7441 gaacatcaac gccagcttcg ctgtttttga ttgattgaat ggttaattga ttaggctcgc
     7501 gtaaatcaaa cggcaatgcc ttttcacgcg cgacctttgt gagggttatg cgaaccaggt
     7561 cagagatggt cagccccatc ccggccagta cgtccgccgc ctgattcttc agatcttcat
     7621 cgattcgggc gcgaacaaat gcgttagcag ccattggggt atctcctgtg ctgtatattt
     7681 attcagcttg aattgtagct caaatgagca acagcgacaa tccatgagta atttaataaa
     7741 cggatgtttt tacataatta actgaaatat atttattaat tttataataa tggtttatta
     7801 agtttgttca ggtacgggcc gggatacact gcaacatcaa tgcatgatca gccattattg
     7861 agtatgtcct tgccgtcgat tccctctttt gtgttgtcgg gattactgtt gctttgcttg
     7921 ccgttttctt catttgccag cgccaccaca tcacatatca ctttcagcta cgccgcccgc
     7981 cagcggatgc aaaatcgtgc gcgtttatta aaacagtacc aaacccatct gaaaaaacag
     8041 gccagctata ttgtggaagg caatgccgaa agcagaaggg cgctacgcca gcacaaccgc
     8101 gaacagataa aacagcatcc agaatggttt cctgcgccgc tcaaggcgag tgacagacgc
     8161 tggcaggcgc tggcggaaaa caaccatttc ttaagcagcg accatctgca taacatcacc
     8221 gaagtagcga ttcaccgtct ggagcaacag cttggcaagc cttacgtatg gggcggcacg
     8281 cggcccgatc aaggcttcga ctgtagcggg ttggttttct atgcctacaa caagatcctt
     8341 gaggcgaagc tcccacgcac ggccaacgag atgtatcact accatcgggc aacgattgtg
     8401 gcgaataacg acctgcgccg gggagatttg ctgtttttcc atatccacag ccgcgagatt
     8461 gccgatcata tgggggtgta tctgggcgat ggccagttta tcgagtcgcc gcgcaccggg
     8521 gaaaccattc gggtaagccg gttagccgaa cctttctggc aggaccattt tttgggcgcg
     8581 cgcaggattt tgacggaaga gacgattttg taaatgggta atattgacga actggcaaaa
     8641 ggccggatgc cacgcggcat ccgacttgtt tacatatggg ctgaaaactc acgttctaag
     8701 acatcaacaa tgagctggtt ttttgaaatg gaatgttgtc tggcgacgat ggtcagccgc
     8761 gaatcaatgt gatgaggaac acgaagcgtc aggcgttttt gctcttcttc ttcacgaaaa
     8821 ggggcaatac cgtcctcttc acaatccgcc atccactcat ccagcgcgct ttcgccctca
     8881 cgatataatg cctcaatgct tgtggctaca aagtcacaat aaccgtttac attgaggaac
     8941 ttgccgcgaa atgcgttgat atcggcttca aatgtcactg cggctggttg acctttaatt
     9001 gtgataatgt tcgatgcttt attcataatc tacccctaca cagttaacaa accactcctt
     9061 aagacctgct actgcacctt tatccatcac attctttgga tgtggctcat gtgtactgaa
     9121 tttggtcgtt ccaatctgaa atttccttct ggacccattg ttatttttta ttgttccacc
     9181 caccgcttta atgagcgcaa caacatcatc ccatttaatt ccagattttg gcggctccgt
     9241 cattacatcg tcgaatgttt ttctatggcg ggagcctagc gcgctgacct taatcatact
     9301 atcaatccat gacgtcattg tatggtgtca tcgtagtatg tttgcaatgt gacgtcaatc
     9361 aatggtgtca gatgcatgca aagcatggaa aagaatcctc atcaactcaa cgctcccata
     9421 atcttcaact ccagctcatc cggcacttca ttatacgaca gcacatgcag ccccggcgca
     9481 aacaacctcg catagcgtgc cagcaaaggg cgcaactgcg gcggcaccag cagcaccggg
     9541 tctttccccg ccgctttcat ctgctccttc acctgcggca tcgtactctg gaactggttg
     9601 agcatattcg gatcgaccgg cacgctgtcg agcatcactt tcccgccctg ttgcgcctga
     9661 ttcaccacgt tggtcagcag attttccagc tcattattca gggtatacac cgtcagctcc
     9721 tgcttgcgaa cgaacggatg ggtaatgctg cgccgcagcg ccaggcgcac atcagctgcc
     9781 agcagaatat gatctttggt caccgcgcta ctggcgacca gcacggtggc aatggtgacg
     9841 atatcgcgca gggaaacgcc ttcggtcagc agcgcgcggt acactttcag caactggctg
     9901 taattcagcg ccgcgcttaa atcttccgcc aggcgcggtg ccatcgacga caaacggtta
     9961 tgcaactgcg taatatcgtc atagttaaat aaatcaggaa tatagctacg cacaatctta
    10021 ttcacatgcg tggcaatcac gctggcgctg tcgatcacct gataccccat attcagcgcc
    10081 ttcgctttct gcgcgggctg gatccaggtc accggcatcc cgtacgccgg gtcgttcccc
    10141 agcacgccgt caatttcgcc gtaggtttcg ctggagggta gggccatcag tttatccgcc
    10201 ggaatatccg cttcatcagc cttaatgccg ttgatgaaaa tagcgtactg gctgggcttg
    10261 aggcggaagt tttcccgaat acggatctcc ggcagcagca cgccgttacc gtcggatatc
    10321 acctgccgca cgccgcgaat ccgctgggtg agcgggttgc cctgcgcttt atccaccagc
    10381 gccaccagct tgtagccgag gcttaagctg atgggctcga tcagcggaat ggtttcccaa
    10441 ctgacctgtt gctcgctggt ttcggtgatg gtgcgggtca gcgtttcgag acttttctct
    10501 tccgcttccg ccgcctgcgg gcgtttgctc atccgccagc cggtaaagcc aagcagggcg
    10561 ctgaacagca aaaacggcag atgtggcatt cccggcacta ccgccagtac gaacataatt
    10621 ccggtagcgg tgtagagcac cgacgggctt gccagcaact ggttacgcac gtcatgggcg
    10681 atatcgccgt tgtcgctgac gcgggtgacg ataatcgccg ccgcggtgga gagtagcagg
    10741 gaagggattt gcgccaccag cccgtcgccg atggtcatca gcacatactg ctggaaggcg
    10801 gcgtcggcgc tcaggttgta tttgaagatc ccgatacaga cgccgccgat caggttgatc
    10861 gccagaatca tcatcccggc gatagcatcc ccgcgcacaa atttcgatgc cccgtccatc
    10921 gcgccgtaga aatcggcctc gctggcaaca tctttacgtc gggtttgtgc ctgcgcctga
    10981 ttgatcaacc cggcattaag atcggcgtca atcgccatct gcttaccggg catcgcatcc
    11041 agggtaaagc gggctgaaac ctcggaaata cgctcggccc ctttggtgac gacaataaag
    11101 ttgattgtca acagcgtcag taaatccgag cgtgaaagca ttatcgccgc gctgcacggg
    11161 cagtcgattt ttaacggtgg tgggctgtcg ccgttaaata aaatcagccc gtctcatccg
    11221 ccaaaacctg ccaccgtggc ggtgccggaa gagacggaga aaaaagcgcg tgacgtcaat
    11281 gagaaaacgg tgctgctgaa gaagaaatcc gccaccgagc ttggcgagct ggcaaccagt
    11341 atcaacacca ttgcccgtga tgcgcatatg gaagcgaatc tggagatgga gattgtcccg
    11401 cagggattac gcgtgctgat taaagacgac cagaatcgca atatgttcga acgcggcagc
    11461 gcgcagatta tgccgttctt taaaaggctg ctggtggagc tcgcgccagt tttcgactcg
    11521 ctcgataaca aaattattat taccgggcat accgatgcga tggcctacaa aaacaatatc
    11581 tacaacaact ggaacctttc gggcgatcgc gcgctttcag ctcgtcgggt gctggaagag
    11641 gccggaatgc cggaagataa agtgatgcag gtaagcgcaa tggcggacca gatgctgctg
    11701 gattccaaaa atccgcaaag cgcgggcaac cggcgcattg agattatggt gctgaccaaa
    11761 agtgcgtccg atacgctgta tcaatacttt ggtcagcatg gggataaagt ggtgcagccg
    11821 ctggtgcaaa agctggataa gcagcaggtg ctttcgcagc gaacgcgtta aatgctgaat
    11881 ctttacgcat ttctcaaacc ctgaaatcac tgtatacttt accagtgttg agaggtgagt
    11941 aatgcgtaaa atcattcatg tggatatgga ctgctttttc gcagcggtgg agatgcgcga
    12001 caatcccgcc ctgcgcgata tccctattgc tattggcggc agccgcgaac gtcggggggt
    12061 gatcagcacc gccaattatc ccgcgcgtaa atttggcgta cgtagcgcta tgccgacagg
    12121 gatggcgctc aaattatgcc cgcatctcac cttgcttccg gggcgctttg acgcctacaa
    12181 agaagcctca aatcatatcc gtgaaatctt ctcgcgctac acctcgcgta ttgaaccgtt
    12241 gtcactggat gaggcttatc tcgacgtcac cgatagcgtc cattgccacg gttctgcgac
    12301 cctcatcgcc caggaaatcc gccagacgat tttcagcgag ctgcaactga cggcgtctgc
    12361 gggcgtggca ccagtaaagt ttctcgccaa aatcgcctcc gacatgaata aacccaacgg
    12421 tcagtttgtg attacgccgg cagaagttcc ggcattttta caaaccttac cgctggcaaa
    12481 aatccccggc gtcggcaaag tctcggcggc aaaactggaa gcgatggggc tgcggacctg
    12541 cggtgatgta caaaagtgtg atctggtgat gctgcttaaa cgctttggca aatttggccg
    12601 cattttgtgg gagcgtagtc aggggattga cgagcgcgac gttaacagcg aacggttgcg
    12661 aaaatccgtc ggcgtggaac gcacgatggc ggaagatatc caccactggt ctgaatgtga
    12721 agcgattatc gagcggctgt atccggaact tgaacgccgt ctggcaaagg taaaacctga
    12781 tttactgatt gcccgccagg gggtgaaatt aaagttcgac gattttcagc aaaccactca
    12841 ggagcacgtc tggccgcggc tgaataaagc tgacttaatc gccaccgcgc gtaaaacctg
    12901 ggatgaacgc cgcggcgggc gcggtgtgcg actggtgggg ctgcatgtga cgttgcttga
    12961 cccgcaaatg gaaagacaac tggtgctggg attatgaata acatacaaat aagagactat
    13021 cagcctggcg attttcagca actatgcgct attttcatta gagcggttac gatgactgcc
    13081 agtcagcatt attcaccaca acaaattgcc gcctgggcgc agattgacga atctcgctgg
    13141 aaggagaaac tcgcgaaatc acaagtgcgg gttgcagtca ttaatgcaaa actggtcggc
    13201 tttattacct gcgttgaaca ctatatcgat atgttatttg ttgaccctga atacacccgc
    13261 cgtggggttg ccagcgcttt gttaaaacct ttgattaagt ctgaatccga acttacggtg
    13321 gacgcaagca taaccgcaaa accctttttt gaacgttatg gttttcagac agttaagcag
    13381 caacgcgttg aatgtcgggg agagtggttt attaattttt atatgcgtta taaacagcaa
    13441 cattaaatcc agcttgcaat gaaaataacg caagcctagt atgtgcgcgt tttcttattc
    13501 cacaaactgc aaggaggtaa atcatgacaa atcctttatc aatgactctt tgcagacctt
    13561 tccaggatta attctttttt tcttgccctg gattcgtctg ccatttcctg attttttaat
    13621 tgttactctc aataattcaa gtcgcaacac atgcaacttg aagtatgacg agtctattta
    13681 cggaatggta tatgggcaat tatattcgtc ccttatccga tgcggtattt accatcgcat
    13741 ctgatgacct gtggatcgag agtttagcga tccaacaatt acacaccacg gcaaatttac
    13801 ccaacatgca gcgcgtagtg gggatgccag atttacatcc cggacgcggc tatccgattg
    13861 gcgcagcgtt cttctctgtt ggtcgttttt atccggcact ggtcggcaat gatatcggct
    13921 gcggtatggc gctatggcaa acagatattc tcactcgcaa atacaacgcc gataagtttg
    13981 aaaagcgatt atcagatctg gatgacgttg ctgaagaaag ctggctggag gaaaacctgc
    14041 cgtcagcatt tgcacagcat ccgtggcgca gctcgcttgg ctctatcggt ggcggcaatc
    14101 atttcgctga actgcaacaa gttgatcaaa tcatcaacgc tgaactgttt gcactggcag
    14161 gtctggatgc acaacatctg caactgttgg ttcatagcgg ctcgcggggg gtggggcagt
    14221 ctattttaca gcggcatatt gcctcgttct cgcatcatgg tttgcctcaa ggcagtgacg
    14281 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgtgt attaatcgcc
    14341 agatgatcgc tttgcgcata atgcaacaag ttaaggccac gggaagtccg gttctggatg
    14401 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
    14461 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcacgt ggtgattact
    14521 cctggattgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggcag
    14581 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acggcgacgc
    14641 aactctcgcg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
    14701 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggccg
    14761 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
    14821 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
    14881 agtgaaaaag gcgctggaca ggctaattaa agaagctgcc cgacaagacg tcgcggtaac
    14941 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
    15001 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
    15061 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
    15121 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
    15181 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
    15241 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
    15301 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
    15361 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
    15421 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
    15481 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
    15541 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
    15601 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
    15661 caggcccgcg tggataatct ggatgttcgg tgttttgttg aacagacgct gataggtttc
    15721 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
    15781 cgcttcggtt ttcgcgccag ctagtttacc cagcgaatcc agcatgctca ccacgtagtc
    15841 tttaccgctg tcgatcagtg aacggatcag gcagtgaatt tctacgttat tgtcagtcat
    15901 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt cggaattgcg
    15961 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
    16021 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
    16081 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
    16141 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
    16201 gccgccgttg aaatcgataa ggcgcagatc cagttcttcc gcatgacccg ccaggaagcg
    16261 caccagtagt ttgttggcat tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc
    16321 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
    16381 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
    16441 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
    16501 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
    16561 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
    16621 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
    16681 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
    16741 gacgaccggt ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
    16801 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
    16861 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
    16921 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
    16981 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
    17041 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
    17101 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
    17161 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
    17221 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
    17281 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
    17341 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
    17401 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
    17461 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
    17521 aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa accggctggt cgtccgctgg
    17581 ttgatgatta tgttgttgat atcccgcaag atacctggat cgaacagccg tgggatatgg
    17641 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
    17701 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
    17761 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
    17821 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggct
    17881 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
    17941 cgcggtattg acccacgtga aatcctagac gtccaggcac gtattgtgat gagcgatgct
    18001 gaacgtaccg acgatgattt atacgatact gtgattggct accgtggtgg caactggatt
    18061 tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
    18121 caactcagtg gtcgacactg gctgcatgcg gctacgttgt acaacattgc cgcctatcct
    18181 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa
    18241 gaggccgccc agcgtctgcc gggcacgatg cggcagatgg agtttactgt acccggcggt
    18301 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
    18361 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
    18421 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa
    18481 tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac
    18541 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
    18601 gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
    18661 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
    18721 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
    18781 ctgaatcgct attcattaaa agtgcaagga ttgctgggac gtcgctgccc aacgccaatg
    18841 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
    18901 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
    18961 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
    19021 aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat gacgttaccg
    19081 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
    19141 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
    19201 aaaccggcac cggaagtgcg tgaattctgg ggctggtgga tggagctgga agcgcaggaa
    19261 tcccgtttta cctacagcta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
    19321 gttccggtaa aagatactga agtggttgaa cgactggagc acaccctgcg tgagtttcac
    19381 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
    19441 cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat cacatccggc
    19501 aatatctatt aaaactgata cgtcatccca atcgcgacaa tatcatcatt attaatattc
    19561 aatttgttat cgctatccag ttggttgatt ttataatcaa caaatgctga catatttttg
    19621 ttgaaataat acgtagcacc gacgtcgata taattaacca gatcttcacc accgatacct
    19681 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
    19741 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
    19801 gtcattttgc gtgtttcaga ataaaaagtt gccagataaa tattattggc atcgtatttc
    19861 agacctgtag cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
    19921 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
    19981 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
    20041 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
    20101 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggag
    20161 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
    20221 ttacggccat agtcgaaaga acccaaatct ttatacttca atccggcaaa agcgagacgc
    20281 gttttttgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaacgg
    20341 ccatagccag tcagttgatc gttaatttgt gtttcgcctt taaaaccaaa acggatataa
    20401 ctctggtcgc catctttact gtcgttatca ctcatataat gcatggcttt aactttgcca
    20461 tagacatcca gtttattacc gtctttgtta tatatttctg cggcctgtac ggatgcagat
    20521 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
    20581 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
    20641 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
    20701 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
    20761 tgctttgctt gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
    20821 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
    20881 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atatcgttga
    20941 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
    21001 gggcgcgatc gccgccggac gtgagcacct ggggtacccg gaactgccag cgactatcgc
    21061 ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg attcaactgt gggaacagct
    21121 gttttcgatt tatggcattc acgtcgggca gatgctgctg acccgtgctg atatggaaga
    21181 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
    21241 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaggtcg gcgataacga
    21301 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
    21361 tcaaaaaggt ttgtataccg ctgatccgcg cagcaatccg caggcagaac tgattaaaga
    21421 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
    21481 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
    21541 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
    21601 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
    21661 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattcttga
    21721 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
    21781 tgaagtcatc cgcatttgca acctcgaagg tcgcgatatc gcccacggcg tcagtcgtta
    21841 caacagcgat gcattacgcc gtattgccgg gcaccactcg caagaaattg atgcaatact
    21901 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
    21961 aggctgatgc tggaacaaat gggcattgcc gcgaagcaag cctcgtataa attagcgcaa
    22021 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
    22081 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgtgc caatggcctt
    22141 ggcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgat
    22201 gatgtgcgcc aggtgtgtaa cctcgccgat ccggtggggc aggtaatcga tggcagcgta
    22261 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
    22321 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
    22381 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
    22441 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
    22501 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
    22561 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
    22621 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
    22681 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
    22741 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
    22801 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
    22861 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
    22921 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
    22981 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
    23041 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
    23101 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
    23161 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
    23221 ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa ggccattgac gcatcgcccg
    23281 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
    23341 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
    23401 atcggcacca ttaaaatcaa agagttaccc catatttaaa tacaccacgt ttcctcctgt
    23461 gccgcatttg tgccattgta accttggcaa ttcatcaaaa tactgttctg acatcaggca
    23521 gtgcaggtgc agctatttaa gccaattgct gccgccattc tttgacgtag tcaatcaggg
    23581 tgcggagctt tggtgcaata ttgcgacgct gtgggaaata cagatagaag cccggaaatt
    23641 gtggaagaaa gtcatcaagc agcgatacaa gcttaccgct ttcaatatat ggcctgaaag
    23701 tttcctgagt ggcaattgtt attcctccgc cggcaagaac cagcctcaac atcagacgca
    23761 gatcattagt cgtaatctgc ggttcaatcg caaggtcgaa agttctcccg ttttcttcaa
    23821 atggccagcg ataaggcgca acctccgggg actgacgcca gccgatacac ttatgggtat
    23881 ttccctcgga ggcgagaaag cgctttccac gcccggccgc aaggatcagg acgacggggg
    23941 caggcatgaa tcctcctcct gatggagacg tacagaggcg atttctgcca gcacggagag
    24001 tgccagagta tgcgcatccc gggctttggg gaatatcccg acgggggccc ggatttgcgc
    24061 tgtctcctcc ctggaccatc ccagctcgtg gagcttttgc agacgtaacg tgtgggttcg
    24121 atggctgccc aatgcgccga gataaaaggg ttttgcttct cgcgcggcct gcaacactgg
    24181 cagctcccgg ttgagatcat ggcacagcaa aatgaccgcc gtatcggtat cgatctgagc
    24241 gctggctgag gccggaaaaa gatcgaagat atggctgtca tagcctgtgg ctgctgcaag
    24301 actcgcggtt gcctgcgcct caagagaacg tccgtaaatc atcagcctga cgcatggcct
    24361 gaaccccacc tcaaagccat tgagattcca gcccgttcgg gtttgcgtgg gcaggcacac
    24421 caacgattgt gcttgcggat cgtagcgcag ccccgccggt tttctctgtt ccaggcggtt
    24481 cagcacggcg agcagaggct gtgccgagcg tagtttatgg agcgtcagcg tgatcccacc
    24541 gccgcagggc agaacgatgt caaaccacgg cgaaccttcg ccatagcgaa tttcgcgatc
    24601 gcggcctgag cccatcatct ccagcgcttc aaaagcggca gcggcctcca cgcagccgcc
    24661 agagacaaaa ccgcagtaac gaccatcttc gcgcaccacc atctgcgccc cgagcgggcg
    24721 cgccgcgccg ccgcgtatct ccaccagcgt caccagcacc gctcctttac cggacattag
    24781 cgcctctacg gcgaagcgca gaatggtccg gtggtcatcg gtgagaaacg cctgctctgg
    24841 cttatgccag tgttcgtctt tatcaaaaag cgggtatgac attgtttctc cttaaaccac
    24901 atccggcagc ttatcgagca gcttatccag agtgatggga taatcgcgta cccgaatacc
    24961 ggtggcgtta tacaccgcgt tggcgatagc cgcgctcacg ccgcacaggc ccagctcacc
    25021 gacacccttg gccttcatcg gggaggatat ggggtcggta tcatccagga aaatcacctc
    25081 ctgttttggg atatccgcat ggaccggcac ctcatacccc gccatatcgt gattaacgaa
    25141 gtagcccaaa cggtcatcca ccgccagctc ctccatcagt gccgcgccca tgcccatagt
    25201 cattgcgcca atgacctggc tgcgcgcagt tttcggattc aggatgcgtc ctgcagcaca
    25261 cacagcgagc atacgccgga cccgaacttc tcccgtcgcg ctatgcacgc cgacctccac
    25321 aaaatgcccg gcaaaggtcg actgctggta ctctttgctc agtgttccga attcaatgct
    25381 ctcttccgct gtcagtctgc cgcctgcggt ggcttcatgt agcgtggcgc ttcgggtacc
    25441 gttggtaatc ttgccgtcgg caaactgcga ctgctcagga tcaaacccga ctgccgaggc
    25501 aatcatttcg cgaagcttca tacaggcggc gtaaacgccg gaggtggagg tattcgcgcc
    25561 ccattgtcca ccagaacccg cagaaaccgg gaaactggaa tcgccgaggt gaaccgcaac
    25621 ctgctccagc ggtacgccaa gcatttccgc tgccgtctgg gccagaatgg tgtagctgcc
    25681 ggtgccaatg tcggtcatgt ccgtttctac ggtaacggtg ccgttttgtt cgaggtgaac
    25741 ccgagcaccc gatttttcca gcagattatt gcgaaagccc gccgcaacac cgtggccgac
    25801 tagccactcc ccgtcgcgca cctgtccggg tgtggcgttg cgctgcttcc agccaaattt
    25861 atccgctccg gtgcgcaagc actcgataag ctgacggcga gagaagcagc gcgtcgggtc
    25921 ggcggggtca acctgagtgt cattcaggat gcgaaactcg acgggatcga tgcccgcttt
    25981 ttccgccagt tcgtcgatcg cgatttcgag cgccatcaga ccgggggctt cgccgggcgc
    26041 acgcatggcg ttcccttccg gcaaatcaag cgtggcgagc cgcaggccgg tatgacgatt
    26101 cgccccggcg tagagtaatt cgctttgctg taccgccgtt tccggcgtgc cgccgggcag
    26161 gtttccagac cagctttcat gtgagatagc ggtgattttc ccgctctggt cggcaccgat
    26221 acgcaagtgc tgaagggtgg cggggcggtg cgtggtgtta ttgggaatag aggggcgggg
    26281 gagcatcact ttaaccggac gtttcaccgc tcgggcggcg agggccgcca gcagcgcatc
    26341 gcttctcagg aacagcttgc cgccaaaccc tccgccgata tacggggaga taatacgcac
    26401 attctccacg ggaactttca gcgtttttgc cagatcggtg cggcaccagt caatcatctg
    26461 atttgaggtc cagagggtaa gcttatttcc atcccagacg gccatcgagg catgcggctc
    26521 catcgccata tggctctggt ccggggtcgt gtaggtagca tcaatcttca ccgcagcgga
    26581 ggtgaaagcc ccgtcaaagt caccgacgtt tttgtcgggc gtgtcttccg gcggctgatt
    26641 gacggcctgt ttttcgtccg ccagggagta agctccttta ttacggcgat agtgtgcctg
    26701 caccagcgag gccgccgctc gcgcctgttc gaaggtctcg gccactacca gcgcaatggc
    26761 ctgatgatag tgctcaatag tggggccgcc taacagcctg gcggtgtttt tgtcgccttt
    26821 gccgagtgcc ccggcgttac tggcggtaat gacagccagt acgcccggcg ctttttgcgc
    26881 ggcgtccgta tcaagggcgg tgaggcgtcc tttggcaatg gcggaaccga cgatatagcc
    26941 ataggcggcg ttgggggcct cttcatgcca ttcgtaggcg tagcgtgccg tgccggtagt
    27001 tttcagcggt ccgtcgatgc ggtcatgggg acgaccgaca accttcagct gatcgatcgg
    27061 gttttcccct gcgggtttat caaatttcat gcctgtgccc tcgcttcagc cagtacggag
    27121 gcaagcgttc gcttcgccaa caggagttta aaggtgtttt cagcggtggg atgggcgctg
    27181 gcgaacagcg tgtcatatac ggcctgcgcc ccctgggata gctgagcatc cgcagcctca
    27241 atgcgccagg gcttatgtgc tactccgccc agcgcgacgc gcccgctgcc gtcaggctga
    27301 ataatcgccg cgaccgatac cagggcaaag gcgtaggagg cgcgatcgcg caccttacgg
    27361 tagatatgtt ttccgccgag cggtggaggt aacgtcaccg caacgataag ctcaccggga
    27421 agcagggcgg tttcaatgtg cggcgttttc cccggtgggt gataaaaatc agccagtgtg
    27481 atactgcgag tctttccctc cggcgtgatg gtttccacca ccgcatccag caaccgcatt
    27541 gcgaccgcca tatcgctcgg atgggtggct atgcaggctt cacttacgcc taccaccgcg
    27601 tgctgacggc taaagccttc aagcgccgcg cagccgctcc cgggcaggcg cttattgcag
    27661 ggctgattgg tgtcgtaaaa ataggggcag cgcgtgcgct ggagcagatt acctgcggtg
    27721 gttgcctgat tacgtaactg accagatgcg ccagcgagca gggcgcggga gagtaccgcg
    27781 taatcacgac gcacgcgctc gtgagccgcc aggtcggtgt tccgtaccag tgcgccgatg
    27841 cgcagcccac ccgcgtcggt cacttcaatc ttatcgagcc cgaggccgtt cacatcgata
    27901 aggtgggtgg gcgtttcaat ttccagcttc atcaggtcca gcagattggt cccgcccgcg
    27961 ataaattttg cgccgggtac gcgctgagcg ctaagtgccg cctcggctgg ggtgttcact
    28021 cgttcatagg taaacgcctt catgatttta tctcccccgc agcatcttca atggcggcaa
    28081 ggatgttagc gtatgcacca cagcgacaga tgttgccgct catacgttca cggatctcat
    28141 cggcagttgt ttctggagcg gaaaccaaat cgaccgtgac gtgactggga atgccgtcct
    28201 gaatctcttt tagcaccgct actgatgagc aaatttgccc ggaggtgcag tagccgcact
    28261 ggaagccatc atgcttgata aaggccgcct gcatggggtg aagattatct ggcgaaccca
    28321 ggccttcaat ggtggtgatc tcggccccct gatgcatgac tgcaagcgtc aggcaggcat
    28381 taagcctgcg accattgacc agcacggtac aggctccgca ctgtccgtga tcgcaacctt
    28441 tcttggtacc gatcaaatgc agattttcac gcaaagcgtc cagtagcgtg gttcgggtat
    28501 ccacctcaag ctgctcggtt ttgccgttca ccttcagtgt caggggcatt atctctggcg
    28561 cgggtgtagc tgccggaacg cttgccgcca gcgtagaatg aggataaacc acggcggtcg
    28621 ccgctgttgc ggcgctcact ttaatcagat cgcgacgggt caaacttaaa tcgtgcggct
    28681 cgtgcttccc aacccgatta tcttcggggt attcgccttg gttgctcata ccaggcctcc
    28741 ggtattctaa aggggaaaat aagagtgcgt tgcgagagtg ctgatgcttt tttaaatgtt
    28801 aagcatagtc ggcgacagca ggaggattat tcttaaaacg cgaatgcagc tatgagcccg
    28861 gctaataaat tcatgttcgc cgggatgttg atgatgatgg gagctggttt attgctttag
    28921 ttgtacgatg caaaaaccaa taaggaaacc tgtgattttc agctctgcat caccctgcaa
    28981 atctctgtca cttctaatat aaaaataggg agaaatgatg gagcttatat tcattggcga
    29041 ttaggaaacc atcttgttat acaaaacaat acagttcttt acatttgcct tgttttatga
    29101 atactcctga agaggtgtat aacataatgg tacaagcagg gtagatatga atatatttga
    29161 acaaactcca ccgaaccgca gacgttatgg tcttgctgca ttcattgggc tgattgctgg
    29221 cgttgtttcc gcattcgtga agtggggggc tgaagttcca ttgccgccac gtagcccggt
    29281 ggatatgttt aatgcagcgt gtggcccgga atcattaatc agggctgcag gccaaattga
    29341 ttgctcgcgt aattttctca atccaccgta tatttttctt cgagactggt tggggctgac
    29401 agatcccaat gcggctgttt atacctttgc cgggcatgtc tttaactggg ttggtgttac
    29461 gcacattatc ttttcgatag tgtttgctgt cggttattgt gtggtcgctg aagtatttcc
    29521 aaaaattaaa ctctggcagg gcttactggc aggtgcttta gcccaacttt ttgttcatat
    29581 gatttcattc cctctcatgg gactgacgcc acctctgttt gatctcccgt ggtatgagaa
    29641 tgtttctgaa atttttggac atttagtctg gttctggtct attgaaatta ttcgcagaga
    29701 tttacgaaac agaattactc atgagccaga ccctgagatc cctttaggct caaacagata
    29761 atgcattgaa tgataaaaat ggcgcaaata cagcgccatt tttataggtt aaaaacattg
    29821 ctttttatat tctgatgcag atagtcagtg agtatatcgc gctacttcag gatgatgtag
    29881 atccgaagaa cgctacagaa gagaggcatt gttgctggca aatagaagaa gtatcgggtt
    29941 ttgttacccc tgaaaaacga agccccgcta ttatcgctgg cggggcattg caattaatta
    30001 ttgtatgttt ataacggtgt cagcccgtat ttagcccttg cacgattgca agcctcattg
    30061 acgactccgt tctcaccaga catggcgaat tctctgcatg tggacgatcg atttttatat
    30121 atcgtgcagc aggcattttt gcccggggtt cctgcaaggg caatacatcg ggggtttttc
    30181 tgattggtac cgctcatgca tcggtgaaaa ggggatattt gttcagtgag cctggcggga
    30241 atagtaccgc cagcatcgtc ggcttcggcc cagtaaaaag agacgcggaa aaatgcacaa
    30301 caggcaccac acgtcatgca tggattcaga ttgctcataa ttcaccagta cagctaaaaa
    30361 tcgtaaagag aacagcggta catcgtatgt aagaacgtac cgcggttggc tggggaactt
    30421 tcgatagtgt gagtattgaa tgatttccag ccgttaccga ttttacgtac taattcgtga
    30481 ttaaaccact cgttagcagg ctgcctgata cctttccgtg tctcctgaac actatggtgt
    30541 gcatatgaga aagcccatcg ttacaggtga tattgacgat cgcttccaca tcatttaaac
    30601 gatttttggg gctctgaggt ggaattcttc cctcgaccga tggataagtc ttagtccgcc
    30661 gaagggggct tagccggaca ggaatcgcta atcttaatga atttgtcgtt atagaccaga
    30721 taatgattcc ccggctgacg taactcaggc tgatgaacca cgtctcccgg acgtagatac
    30781 caggcatcgc cttcctcttc ggtcgaatca catcctggct taatcagtag cttaaaccat
    30841 gtgttgccag tgttacttac cgttcctgtc accttgtcga aggaccattt aaactgaacc
    30901 tgacgtggtc gtactaccag aatggtatcc atcaccacca ccggctccgt gctgacctcg
    30961 ccgccggttg ggctacgtct tgtctggtta cgagtgggga cttcgcgaaa tgagacccgg
    31021 tagtagcgct cgcggttatc ccgtggacca tgatagtaaa atttaaaata ctcgctctca
    31081 ccagcctgca acgccagttg gcggggggcg aaaagcagtt caccatccac cggtcgggtg
    31141 cgcaattcac tgctgcccgg gctatcaata gcactgatgg caatacggta tatccgtgcg
    31201 cttttgttgt tattcactac acgtttgctg acaaagtcag tctcggacgg cagcgaaaat
    31261 gtcagattgc cgaccgaaat cgcctgtgtc aaaggtgccg ccagcataca gaccgctgtc
    31321 agcagggctt tagttaatgt tacgccacgt cgcctgaaca tgaatctctc ctgaagcgct
    31381 gacttcgcca aaccagccgt tatcatctac cgtacgcagc gaaatggcgt tatccattgg
    31441 aatcgaaaat ttgactgtgg tcttatccat ctgccccact tcgccagaga tatcagtcca
    31501 cggtgtggtc aaccacagcg catcggtcat atcccgccag ctatcatcgc acccggcatc
    31561 gtaggttttt gtagctccgt tgcgggtaat aaaggaaagt gtcgccggga acggtacttt
    31621 cgccttaccg tcatcggagc tgaagacaca ataggagcgc ccgccaatca cctgcgcggg
    31681 tccggtcacc ttgatcagca cttcgtcggc agctgtttta ccgctggtgg ttacgatata
    31741 gccgaaatcg agtgccgact cgccgctgcc aacatatccc tcccgcgacg gtgtctgagt
    31801 atattcatcg gagataatac tgatgctgaa atcacggggt ttgataatca gcgtgttgga
    31861 ggtagaaaat tcataccagc cagactccgg tgatgtggtg ttctgaaagc gaaagttgaa
    31921 tagatctttg gtgttgatat cgctgatccc gaggttcacc atctgcttaa agaagttgct
    31981 cgagaagaaa acatagatcg agtctgcgct cttcatctcg ttgaaggtgt agtaataggc
    32041 ggtattgctc accggtttcc atgaactgcc attcagactg aactgcatat cgtacgcccc
    32101 gacggccgag gctaacgacg agttggcgat cgccgggaat atatggattg aggtgttgct
    32161 gagtccgttt gtttgcaggg tatagttgac catcttacaa cttaatcctg aaaggctgcc
    32221 gatggtctgt gtccggcagt cggcgttccc ttcgcccaga gtcggtactc cgtcgctgtt
    32281 gataaatacc tccgccagcg agtgggtatt tattaaccgc agatttgctg ctttcgtatg
    32341 ggtgacgttg cgaacatacc agttgccggt ggcctgatcc ttacagcgtg cgccgctgct
    32401 ggcgtcatag ttcacagagg tctggcaggc attgatggtc attgtaaagc tgctgccgac
    32461 gggcatttgt tgcagatact ggtaaaacgc gtctgacaac atgccgtgca tccacttcgc
    32521 gccgccgctg gtcaccgtcg cgccataaaa tccactggcg tcggtggttt gcggcaggat
    32581 aagactggtg gtcatattac acccagcgta ccagttgatg cagcgcaagc cagttaaagg
    32641 agatgaaact ggtgaatttt ccagccacat atcaaacttc cagttggtat aaaggccggt
    32701 gttgtaaccg ttatcaatgt agccgaggct ttgctgatag atggttcctg aaccagtgta
    32761 ttttaaaccg gtccagcggt tggcaccggt caggcgcgga tccagcgccc cgccaggagt
    32821 gacgaaaaag ttgtcgtctg agttgttttc gacaaacaca aactcgcgtg ccggtgcatc
    32881 cgcccaggtt gttttgctca ctgccgctct gagctcagtt actggccaga ttagcgcgaa
    32941 aattatcatc gttattagta ggttaactct cattctcttc tcctgtctgc gtcaccgccg
    33001 cccacgatga gaggccgctg cagaccacat caccgaccca gacggcaccg cgcgcctggt
    33061 tgagttccag tgccacttcg caggttttat tgccactgta gcgaaaatcg atagtgggat
    33121 atttcttatc cacgtccatg acaaactcgc cgttttcatc ggttcgggtt cggccgatat
    33181 ggttgttaat ccgtgcgtta gccagcagtg tgccgtcttc cgcacggata cgaccggaga
    33241 cggtaaccat ctgcttcacc tctggctcaa tgacagcgac attgcctgga tagagagtca
    33301 gatggctttt gcggccgctg acgatatcgt agctgtcgag tgagtttttg ctgttctgta
    33361 actccacctc atatcttcca tagggagaga gcgggagata gttacgcttg ccgttaagcg
    33421 ggaaaatccg cccgttgatt ttggcgctga tctgaccgtc gtcctccagc ccggtgttga
    33481 atatcacccc agcgttgcca tcagtccgcc cgctggcagc gatgttttta ccctgccagc
    33541 cgacgctgcc attggcggtc aagttggtat tgatgtagcc gtccgccgcg ctattgacgt
    33601 tcagcgttcc gctggcgtaa cgagcgtcga actgtgcata cgccccaccg ctgagggttt
    33661 tgtcatcacc ggtatcgccg gagatggcac gtgacagatt ggcaccaaca gtgcgaatgg
    33721 ttccttcatc aaactgtttg cgtgctgaca ggtttgccat ggtgtagccg ttttgatggg
    33781 tcatccctgc gctaaaccag ttgcccagtg gtagcgagag atcgagagcg atatatttcc
    33841 ctgtattggc gttgctgtcg ccgttgttat agcgctgaat accggcccgc aggccaagcg
    33901 aaccaaaggt accgctgtag acattttgat agtaatctgc cgtgtaataa tggctgttgt
    33961 aacggcggtc atcattgtag ctgatgctga atgtgcccag cttcgaccac agtgagttca
    34021 ggttgagtgt gccgccgatt gcacggttgt cggcatcgct acgtcgcaat tgattgccaa
    34081 tgcgggtttt ttcctggtta acccacagcg aactaaagcc tcccggtaga gtggcgctga
    34141 tgctgccgat gctgctccat gagctgtcac tggccagcat attttgcagg ttaacgttga
    34201 tcgccccccc aagcggcagc gtcagacggg tttcacccac cgcctgatta tcgtatccat
    34261 atcccgttgc cgcccagcta agcgtactca atgagccgga ggtcgaggca cctgctagcc
    34321 aactctcttt agctggtcgc gtctttttcc cgttttccga ccagcgatcc atatgaaagc
    34381 taccgcccca tacctgccac gccagtggtg caccgacgcc gcgcccccgg ctaaacagct
    34441 tattgacccg ctgggggcgt ttgctgatca cgcgaccgtt aacgatcacc tcaacttcca
    34501 catcgtaaat cccgtacggt agaccccggg tatccacttc atgattgccc atagtgaagt
    34561 tctgaacgct tagtagccgc ccatcacggg tgagatgtac ttcgcccgcc gccggtaaaa
    34621 aggcgatcac tggcgtggct gactggctgc tgtcgaagat ggtggagctg gcctggtttc
    34681 cccaggaaag gccgtaaatc ttccctgctg aaatggcggt catcggccct aaggactgca
    34741 agttccaggt gtcgagcatt ccaccggcaa atcggtgacc ggcaaaatcg cgttcataca
    34801 tcgctttata taattcactg tcctgttgac cgctaccgat cccgtacagc gagccgtcga
    34861 gcaccacatg gtgttcgcgc agcgcagtaa cgttattcag cgacagatag ctggatgtgt
    34921 tgctcccgcc gttacgcaac tggttgttat agacgcccaa gttatagctc agattactgc
    34981 tgagggtgtt aacactggac tgcccgatgt cttcgctacg tgagcgtagt acggtgccca
    35041 gcgcttcgcg cttgaccacc agctgcagca gcagttggcg caagctgaga tccagctgcg
    35101 cgttgtcagt cagggggatg gtaagggcct cattgaacgg ggcgttggcc agagccatca
    35161 gctgctgtcg agtttgttcg ctgacactgg cgttatcttc actctcttcc agctgtattt
    35221 tccggatgcg tagctgtcca tcatccagcc agataaaagc gctgccgatt cgctgatcgt
    35281 cctggcgacc ctggctaccg gcgagatgaa tatagagcgg gacgctcatg ccgtcctgaa
    35341 gcgcctgact gaaggcctgc ggaataatca ccccacctat ttgctgcgca cttatgtcag
    35401 cagcgctggc atcgggctga acgaacaaaa agaccatgcc gaaggcaaac tgggctttca
    35461 gtcctgggga gaaccgtcgt aaaggcattg tcgttatcca tctgcacagc tatctatttc
    35521 acgggaatga acttatcacc ctgccaaagt gcaacccgtc ctttgttatc cgccgtgtcc
    35581 acgcgggtaa aacgacgcga cttgcccggc atcaggtagt aattctcttt acactcctta
    35641 ccgttggcgg ctttcaggca agggccgtag gcgaggatcc gcagcgtcgc atttcctgta
    35701 tttgtcaggg agccgttggc gtactgaaag tggtagttcg cctggcgggg ggcgacgacc
    35761 agaatggtgc cgatgcgggc ggaagcagtg gccacagcgc tgcggttggc attatcgcgc
    35821 tgcgcatcac tgagggcctg atcaaaccag acaatgcggt agtagcgctc tttttcatct
    35881 gcgggtccct tatagaagaa gcggatcact tcgctggctt gggcgggtag cagcaagctg
    35941 gcgggagtga gtagcaactc atccggcttg tccattgaga taacctgccc gtcgtcaagc
    36001 ggtgaagaga gccgttcgag acggatattg ataaggcgac cactgtcggt actgtttttg
    36061 atcgttttgc tcagcgtgct gctgtcactg ttcataaacg atgatatatc gccgacatcc
    36121 agcgcctggg ccggagatat tccggaaaac agcagagcga gaggcagaag gtgctttttc
    36181 ataacaattc cgtccaggaa taaagcaggg gggctacccc tgctggtaca tcagagagat
    36241 taactggtcc aggtcgcgtc gaactgtacg ctaacgtcgc cgctccagat gccttccggt
    36301 agagtgctgt aatcggttac tgcggtggta ccattggtgg tgccgctgat gatggtgaag
    36361 gtgaaaccat cctgtgcggt ggtacgattg ctggcattgt aaccgttagc cagcgggcta
    36421 aggttgccgc ccagtacgcc gttggcggta tcgatcatca cggtatcgcc agttttttcg
    36481 actgccgcgc cgttataatc cacgcccaca ttcagtgtgg aacctgaggt atccaactgg
    36541 gttaatgtgt tggtgataag acgtgaggtc agtttaaagg cggtagccgt tgagtcaccc
    36601 tcgatagcca cgtcaaatag acctttctgt gagttaaaac ctttaatgcc ttcggcatac
    36661 tggaacgcca ggctaccgag tggcgtcaca accagcttac tggtggtgtc ttttttggct
    36721 gttgccgacc aggtcgctac agcctgagct gttacgtcag cagcctgcgc cacacccatg
    36781 ccggtaaaca ccgttaccag agctattgcc agaacctttt ttttcattgc atttcttccc
    36841 gagttgaatt gaggacgtga tgtcccagtg agtcattttt taaaactaac ttgcctggag
    36901 tttactgaac caacttatat atttttgagt acagcttggc ctcagcatta cgccgatggg
    36961 tatacactgt cttcacacta caattttcaa ttctggcaat cgatttaggt tgcattccct
    37021 gggccgtcat gcggataatt tccatttccc ggtccgtgat tttatctttc ttaatatctt
    37081 ttctcaggaa cctgccatta atcacatagg ccagttcttt tctaatatca cgactcagtc
    37141 cagcgtatac cacgccccta atattgctat tgtaatacca ataatttgct aaggcttctg
    37201 actttctggc cgcaatcaat acgacctgtt tcccctttac cgcggataac cattcattat
    37261 cacggtttat aaattcagat aaagaatccg tattgagatt gataaacacg aaatcttttc
    37321 taattttctc caacgataga taaattaatc tgtcgatatc cacaatcagt tcagatagtc
    37381 ctttatagaa gtaggcgtca tggggagacc agatgtattt atcagaacgg ttttgacatt
    37441 ccatatggtt tttaacctca tagtccctgc tgtaatcact ttgccatgtc actactttcc
    37501 aaacctgtaa tttttacagg tgcattcagt ttccttagta aaccggaagt aaataagata
    37561 cgtagtacat atcaataaag gttaaacgag attaagttta attattttcg attgggctta
    37621 aatttatgtt tttttcccga tgagtgtcaa tatgtgaacg gaaaaggaat tcaatggtta
    37681 agtcaatatt tagaaaaact gaaaatattg cttttttgat aagaagaaaa agacatttgg
    37741 tttcataagg ttattggcaa tataacgttc aattcttact tgcattttat attggtcagg
    37801 aactttgccc tgaagtgtta ggaatagtct taatgcgctg tgtgatggac tagtaaaaaa
    37861 acaagtttta agggtaaggg atagaatata aaactataat gagctgtcat tgaagatgat
    37921 ttgccaatca gtaagaaatt ttaatggatt aacatgccgc acaaaatggt tataggtgtt
    37981 cattacaata agtgatggaa aattgaacgg gcgtatgtac gtcatctgtt cggtaagccc
    38041 ccaagagcca gattaagtga agttaaacga aggctgccag ctgtcacagg aacgatggcc
    38101 tctttgtcag ggcactgagt gcagttatct gtgcgcgtta ctctaactgg cactcgaacg
    38161 tcggcgacgg ggttaccggt ggatcagact gttcctgtgg cgtaaacgtt tttatgttag
    38221 taccaacaga tttaccacat ttatcatctt aacagtttga gtgaaaatgc aggctgcgcc
    38281 gcaaagggag gagactgagt ggcgtgttca gtctaaaaga gagttgatgc cagcatacag
    38341 gggcgaagct ggtcaacaag tcaacattaa gataatggaa tattcggaga gaaacgtcag
    38401 gcagttggcc tcaaatgaac aggaggaata tatccccagg aaaattaatg ttggtgttat
    38461 aaacacacca acattaatcc gatctgatta ttaatcagac aaatatcgtt aatccgtcga
    38521 attttgaatc aacgtttttt cttacgaccc tgaacggcct taaaacgtgg attagattta
    38581 caaatcacat ataaccgtcc ttttcgcttc acaatctgac agtctggatg gcgttctttt
    38641 gcggtacgca gagagttaag aactttcatc acgacccctt tttcgtgcta acaaaacgac
    38701 caaaacgttg ggtgaatcgt gcaacatttc cttctgatgc cactgttctc agcttccctg
    38761 tatagaacgg gtgcgattta gaagagacat caattgtcac gtatggatac gttacgccat
    38821 ccagctcaat ctcacggtct gttttgatag tcgagccgat tttaaagtac tcatcaacac
    38881 tggtgtcgtg gaacaccaca gtacgatact caggatggat attgggcttc atcattttta
    38941 cctgttatgt tataacataa ccataaagat acatgctcat ccggatcatt tcaacaccca
    39001 atccctgtga ctctttgtgg atgtggaatt ttcgcaacgg ggaactctgg cattgttagc
    39061 agccgttggc gacgctccca taaacgcctg gatgagtgat tgatgatggt ttcgctgaga
    39121 gtatggattt ttcgacaatg gaggttatct ccactttact ggccgatgat ttatcgcagt
    39181 atgattgatt acctgacagc gtgtattcct ctcccacgga tataataatt acttacaaaa
    39241 agggagagga tgcatatttt aaatatcact gaagtgaaca gtttattgcc gttattaata
    39301 gaaatggaga aataaatagg cgtattctac aattgcgaca aaaacaacga tattaatcag
    39361 tttatgactg atttgttgta ctttattctc tttcattggt acttcctcgc tttaaaaaag
    39421 agcgcacttc gtaagtgccc ttatataaat aacgagtttg gtcaaccaat tttttgacat
    39481 gtatcacaaa tttgaatgga tgtattacat cggctatctt ttattgcacc aacgtcattg
    39541 atatatgtcg cctgaagtca gttccgggaa tgagtttgat ctcaagactg gcccagtccg
    39601 ggcgttgatt ggcgctgagg agcatatcgc atctcatcat aatgtcgtat ctcctggggt
    39661 gttatacaag atatcgttgt tggtgacctg ggagaggaat tgagttctat taaaccgtca
    39721 actatgccgg atacatactg gattacactg caggcacgcc ttacgagaga acgtgccgca
    39781 gtgacgggtt aattatctga aagaatttgc gaggctgtat cggttactca ttggtttgat
    39841 agttttactc tcgggagagt aatagatatt taattcatta acggaaacca gtcagttcct
    39901 ttcgatgcct gaatttgatc ccatagttta tccggaatgg ttaattcatg aacaagttgt
    39961 gttatcgttc atgagaagca taacgtaaag ggaaaagctc gattagacgg caggatttgt
    40021 caggggttat gaacgaaatt cataaatctg tttgagtgtt gcgatgggta gtgcaagttc
    40081 gaaatctccg caatttacag tccgatgaag gaaaatgaat atccataaaa aatatatcga
    40141 tttattctgg cgtatatacc tacttcgacg tatttccaat agttgttaat taaaggcagg
    40201 ccattattat tcactctgaa taatgaatta ttcactgtcc gcagagtaaa aaatataact
    40261 taggtgtcta tttaatgact tgcacaaaaa gctaaatttt cacccataaa taaaaatata
    40321 atcccgcgcc caaccacctg atgagtggct acagacactg gatatattag gtggcggtgc
    40381 actttcttac ataaaggtat ttccttttct gcggaaaagg aaatcgggaa atccccggtt
    40441 tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt tcgttaattc gttcatcagg
    40501 aaattatctc aatgtcgcgt tataaaacag gtcataaaca accacgattt cgttattcag
    40561 ttctggcccg ctgcgtggcg tgggcaaata tctctgttca ggttcttttt ccactcgctg
    40621 tcacctttac cccagtaatg gcggcacgtg cgcagcatgc ggttcagcca cggttgagca
    40681 tgggaaatac tacggtaact gctgataata acgtggagaa aaatgtcgcg tcgtttgccg
    40741 caaatgccgg gacattttta agcagtcagc cagatagcga tgcgacacgt aactttatta
    40801 ccggaatggc cacagctaaa gctaaccagg aaatacagga gtggctcggg aaatatggta
    40861 ctgcgcgcgt caaactgaat gtcgataaag atttctcgct gaaggattct tcgctggaaa
    40921 tgctttatcc gatttatgat acgccaacaa atatgttgtt cactcaggga gcaatacatc
    40981 gtaccgacga tcgtactcag tcaaatattg gttttggctg gcgtcatttt tcaggaaatg
    41041 actggatggc gggggtgaac acctttatcg accatgattt atcccgtagt catacccgca
    41101 ttggtgttgg tgcggaatac tggcgcgatt atctgaaact gagcgccaat ggttatatcc
    41161 gggcttctgg ctggaaaaaa tcgccggata ttgaggatta tcaggaacgc ccggcgaatg
    41221 gttgggatat ccgcgcagag ggctatttac ctgcctggcc gcagcttggc gcaagcctga
    41281 tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg taaagataag cgccagaaag
    41341 acccgcatgc tatttctgcc gaggtgacct atacgccagt gcctcttctg acactgagcg
    41401 ccgggcataa gcagggcaag agcggtgaga atgacactcg ctttggcctg gaagttaatt
    41461 atcggattgg cgaacctctg gagaaacaac tcgatacaga cagcattcgc gagcgtcgaa
    41521 tgctggcagg cagccgctat gacctggttg agcgtaataa caacatcgtt cttgagtacc
    41581 gcaaatctga agtgatccgt attgctctgc ctgagcgtat tgaaggtaag ggcggtcaga
    41641 cactttcctt ggggcttgtg gtcagcaaag caactcacgg actgaaaaat gtgcagtggg
    41701 aagcgccgtc attactggct gaaggtggca aaattaccgg tcagggtagt cagtggcaag
    41761 taacgctccc ggcttatcgt ccaggtaaag acaattatta tgcgatttct gcggttgcct
    41821 acgataacaa aggcaatgcc tcaaaacgcg tgcagacaga ggtggtcatt accggagcag
    41881 gtatgagcgc cgatcgcacg gcgttaacgc ttgacggtca gagccgtatt caaatgcttg
    41941 ctaacggtaa tgagcaaaga ccgctggtgc tgtctctgcg cgacgccgag ggccagccag
    42001 tcacgggcat gaaagatcag atcaagactg aactaacttt caaaccggct ggaaatattg
    42061 tgactcgtac cctgaaggcc actaaatcac aggcaaagcc aacactgggt gagttcaccg
    42121 aaactgaagc cggggtgtat cagtctgtct ttactaccgg aacgcagtca ggtgaggcaa
    42181 cgattactgt tagcgttgat ggcatgagca aaaccgtcac tgcagaactg cgggccacga
    42241 tgatggatgt ggcaaactcc accctgagcg ctaacgagcc gtcaggtgac gtggttgctg
    42301 atggtcagca agcctacacg ttgacgttga ctgcggtgga ctccgagggt aatccggtga
    42361 cgggagaagc cagccgcctg cgacttgttc cgcaagacac taatggtgtt accgttggtg
    42421 ccatttcgga aataaaacca ggggtttaca gcgccacggt ttcttcgacc cgtgccggaa
    42481 acgttgttgt acgtgccttc agcgagcagt atcaactggg cacattacaa caaacgctga
    42541 agtttgttgc cgggccgctt gatgcagcac attcgtccat caccctgaat cctgataaac
    42601 cggtggttgg gggtacagtt acggcaatct ggacggcaaa agatgcctat gacaaccctg
    42661 tgaccagcct cacgccggaa gcgccgtcat tagcgggtgc cgctgctgta ggttctacgg
    42721 catcgggctg gacaaataat ggtgatggga cgtggactgc gcaaattact ctcggctcta
    42781 cggcgggtga attagaagtt atgccgaagc taaatggaca ggatgcggca gcaaatgcgg
    42841 caaaagtaac cgtggtggct gatgcgttat cttcaaacca gtcgaaagtc tctgtcgcag
    42901 aagatcacgt aaaagccggc gaaagcacaa ccgtgacgct ggtggcgaaa gatgcgcatg
    42961 gcaacgctat cagtggtctt tcgttgtcgg caagtttgac ggggaccgcc tctgaagggg
    43021 cgaccgtttc cagttggacc gaaaaaggtg acggttccta tgttgctacg ttgactacag
    43081 gcggaaagac gggcgagctt cgtgtcatgc cgctcttcaa cggccagcca gcagccaccg
    43141 aagccgcgca gttgacggtc atcgccggag agatgtcatc agcgaactct acgcttgttg
    43201 cggacaataa ggctccgacc gtcaaaacga cgacggaact caccttcacc gtgaaggatg
    43261 cgtacgggaa cccggtcacc gggctgaagc cagatgcacc agtgtttagc ggtgccgcca
    43321 gcacggggag tgagcgtcct tcagcaggaa gctggacaga gaaaggtaat ggggtctacg
    43381 tgtcgacctt aacgctggga tctgccgcgg gtcagttgtc tgtgatgccg cgagtgaacg
    43441 gccaaaatgc cgttgctcag ccactggtgc tgaacgttgc aggtgacgca tctaaggctg
    43501 agattggtga tatgacagtg aaggttaata accaactggc taatggacag tctgctaacc
    43561 agataaccct gaccgtcgtg gacagctatg gtaacccgtt gcaggggcaa gaagttacgc
    43621 tgactttacc gcagggtgtg accagcaaga cggggaatac agtaacaacc aatgcggcag
    43681 ggaaagtgga cattgagctt atgtcaacgg ttgcgggaga acacaatatt tccgcttcgg
    43741 tgaatggtgc tcagaagacg gtcacggtga aatttaacgc ggatgccagc accggtcagg
    43801 caaacctgca ggtagacacc gccgttcaaa aagtggcaaa cggcaaagac gcctttacgc
    43861 tgacggcgac cgttgaggat aaaaatggta accctgttcc agggagcctg gtgaccttta
    43921 atctgccccg gggtgtcaag ccgcttacag gcgataatgt ctgggtgaaa gccaacgatg
    43981 aggggaaagc agagttgcag gtggtttcag tgactgccgg aacctatgag atcacggcgt
    44041 cggcaggaaa tgaccagcct tcgaatgcgc agtctgtaac gtttgtggcg gataagacta
    44101 cggcaaccat ctccaatatt gaggtgattg gcaactatgc gctggcggac ggcaaagcca
    44161 aacaaacgta taaagttacg gtgactgatg ccaataacaa tttggtgaaa gatagcgaag
    44221 tgacgctgac tgccagcccg gcttcgttaa acctggaacc gaatggcact gcgacaacga
    44281 atgagcaagg gcaggctatt ttcaccgcta ccactattgt tgcggcgaca tacacactca
    44341 aggcgcaagt gagtcagacc aacggtcagg tatcaacgaa aactgccgaa tctaaattcg
    44401 ttgcggatga taaaaacgcg gtactcaccg catcatctga tatgcaatct ctagtggcgg
    44461 atgggaaatc gactgcgaag ctggcggtga ccctgatgtc ggcaaataac cccgttggcg
    44521 ggaatatgtg ggtcgacatt cagacgccgg aaggggtgac ggagaaggat tatcagttcc
    44581 tgccgtcgaa aaatgaccat ttcgtgagcg ggaaaatcat acgtacattt agtaccagca
    44641 agccaggcgt ctatacattc acatttaacg ccctgaccta tggcgggtac gaaatgaagc
    44701 cagtgacggt gaccattacc gcggtggatg ccgatacggc aaagggcgag gaggcgatga
    44761 aataattttt aagaatatga aaatagcatt ctaataaaca ataatggcgt tgacatattc
    44821 aacgccatta tttattgaat tgcctgacag tagtctcgtt ataagttcat ttagatatag
    44881 cgacagctaa accggacgaa cggggataaa aatttcgcaa acaacatcat tgctatcctt
    44941 ttctacgaaa tcttcattgt aataaaatcg ctcaatatca ctgccgttca ctctggctaa
    45001 ccccctggca ggtaattcca tcagatagag gtttcgcgac cattcaggat agcgctccca
    45061 ctcaccttca tagcggaaag atgcatacag tcctcggttt attgtccact ctttcgccat
    45121 ctcagaatcg gtttgtttac ataccgtacc ggcaaaagtt tcaactgtga gcaaatcgac
    45181 cgattttgtt gacggttcaa acgtggagac gaaatagata tccgtcttaa aattattcat
    45241 acatctggct aaatgatgac gtcgcagttt gacttcttca tccggtgatt tattcgacac
    45301 ttcaatgtca tatttaaatg agttgccaaa aataggtgtc tcaggaaaat tgactaatct
    45361 acactccgtc ttttgctgtt gacgtattaa aaaagaaggg aagatatttg cgagatccca
    45421 ataatcacgg tggcggtatt cacggggaga gcagccgaat aactttttaa attcgcggct
    45481 gaatgactgc tgtgaatcaa aatgcaaaga gagtgcaata tcaagcatag atttcgcggt
    45541 gagccggaca agaatagcgg cacggcaaag ccttcgtttg cgaatgtatt ctcccaaagg
    45601 cacatgcatg aaatttcgga acagaagctg gatgttgcgt ctgctgtagc cagatttctg
    45661 tgcgatatct tcgattgaaa tagggtgctc aagattgcac tcaatccact ccaggagctg
    45721 ctgtagaatc ttctgcctga tcatcacatt ctccgttaat tctttgtcat atcgcacatt
    45781 ttcgttatgc cctgggccct aagtgtatca aaaacagcaa gggcgaaggc aaaagcactg
    45841 gtagcgtctg cttcgttgtc cccgaaaata gagaggtttc agtcctgcat tattaatgat
    45901 ttttttgcat aagtgatatc aaaatccacg tactaatttg aggttacgtt ttaacgtaga
    45961 ctcattgttc atgcctaatg gagggactga cagtggaatt ttccgtatta agtaacaatc
    46021 tgaaaatgcc gatgatggga tttggtgttt ttcaggttac cgataaaaac gtgtgcaaac
    46081 agtcagtgtt gaacgctatc cgcacaggtt atcgactcat cgacactgca gcggtatacg
    46141 gtaatgaaga tgccgtcggt gaagccgttc gtgaagctat atctgaaggt ttatgtaccc
    46201 gagatgagtt atttataacg tctaaattgt gggtgcagga tatgttgaat caggatacgg
    46261 cagcagcagg tattgaagca tcattaaaaa aatcgggact agagtacttc gacctctatt
    46321 tattgcacca ggctatgcgc gattatttca gtgcgtggcg tgcactcgaa gatgcctatg
    46381 aagagggcaa attaaaagca attggggttt ccaatttcta tcctcatgtt ctggcgaact
    46441 tttgtgaaac ggtaagagtt aaaccgatgg tcaaccaggt cgagttgcat ccctattttg
    46501 cccaaccaga ggcgctggca accatgaagt attataacgt gcagcctgaa gcatgggctc
    46561 cgttaggtgg tggacgacat aaaccctttg aaaataatct gcttcagagt attgcagatg
    46621 cccatcaaaa atcgatttct caagtcattc tgcgttggaa tattcaacgg ggagtggtcg
    46681 ttattccgaa atcgacacat caacagcgta tcgaagaaaa ttttgctatc tgggatttct
    46741 cactgacaga gaaagaaatg gcacaaatta gttcgcttga tttgggttat gttggggagt
    46801 cggtaaaaca ttttaatcct gaatttgttc gtggttgtct tgctgtaaaa atacacgatt
    46861 gatattaatc atatatttta cctgagacga caagaatctt ttaaccgggg agtgatattg
    46921 atcttcactc tgtcatatct ccggtaatat ggcgtcaggc ttcatcatca gaaagggggc
    46981 ctgacgcctg aaaaagtgaa caacagacag tgttcggatt atcaatattc agtttttaac
    47041 gttgaactgg attcattact gcgttgttta agaatttccc gcgccgaatc tgccattatc
    47101 atgacagcac ctgccagcat cagagtatct ttcaatacca ggcgaccagc accggataaa
    47161 taagggaaac catgatgagc gtcacccaat gcgggtaccc atgcctccgg ggtggtgatt
    47221 aaaaatgaga gtgttaccag cggcgtggta aatgccatca gtcctcccaa taaacctaac
    47281 cagcgattga caggattagc caaaaccagc agagcaataa tcacctccac gacgccaaga
    47341 ccgttggaaa aaccataggt attattggcc gtttgccatg cccttgcttc tggtttgtat
    47401 tcgccttcgt gagtcagata ctgtttatag tcttccgggt gttcataaaa gaacgacatt
    47461 agtggactgt ttgcgacgaa tggtgtaatg ctgtctgcct cgtaagggac aaactttaat
    47521 aacccaatcc acataaaaac aattgcaata ctcagacgaa tcaatgtcag gccaatttta
    47581 tctccccgac ttaacaggtg caggtatttt tccattgtga acatccttat ttatacaata
    47641 ctgctgttgc ggttgaatta ttatcaattc gcatcgatgt gactttccag gatgaagcat
    47701 tatattttct ggctatatca gcaatggcat cctccagaac tgtcggtgat gctgatactg
    47761 acgagacgct gatatgtttc agaattattt tatcatctgc attggtggaa aatgccataa
    47821 cgccagataa tagtgttgca aataaaacag attttttaaa catataaaat accatcaatg
    47881 aagtgattaa gaagttatag agtaacaggg aattaataag attcttttct ctgagacgcc
    47941 agaatatttg ttctggcgtc tgattttgag tttatttgac taatgaaaat agatcattga
    48001 gtgattcgct catcgacgga tgagtaaata tctgatcgcg taatatgcta taaggcagcc
    48061 cggcatccat caccattttc actatattga tcatctcgtg ggagtcaaca cacagcagtg
    48121 atgcccctaa tatacgttgg gttttattat caacaatcgc ttttaatacc ccacgagtat
    48181 cattcatcac tctggcacgc ggaattgcag ctacaggcaa tgtcaccacc tgaatatcag
    48241 caccactctc tctggcctgt tcttctgtca taccaaccct ggacagtggc ggtgtcatga
    48301 atacggaata aggcacattt ttccgatcat cagtactacg tctgccttct cccagcaact
    48361 catcacgcac aatgcggtaa tcatccagtg aaatataagt aaattgcagc ccgccggtaa
    48421 catctcccat cgcccaaata ttgtctgcgg tggtatgtaa ttgcttgtca acgacaattg
    48481 ccccgcgctc gtttactgcg ataccggcat tttctggatg taacgaagcg gtagccggtt
    48541 gacgaccgga agctattaac agtgcatcca ccgccagttg ggcgtgctcg ctatgcactt
    48601 gcacttgatt ttcatggtga ctgattcgct ccacatgggc attgaggata atatcgacgc
    48661 cctgatcgcg taaaatcgtc gcgatattat cagcaatatc ccgatcttcc cgaggcaaaa
    48721 acagcgaagc tgcttctaaa atggttacct tgctgccaaa attagcgaac atagaggcga
    48781 actcaacgcc aatatatccg ccgcccaaaa tacctaaatg cccaggcaat tcttttagat
    48841 taagtaatcc ggtgctgtca tatactcctg gcgtggtggt aattccagga attggcggaa
    48901 ccacggtttg tgcaccggta ttaataaaaa ttttctcgcc atgaatttcc agatttgcct
    48961 caggccgatg aacacgcagg ctatgattat tgataaactc cgcctggccg tcgatcacgt
    49021 cgatattggg catatccgca agattatgaa aattcttatt acgtaaaaaa ttaaccactt
    49081 catttttacg ctgtatggca cggacaaaat ctgtgtgctg ctgtgcgtca tgaaccaatg
    49141 ttttggttgg gatgcagccg atattaatac aggtcccgcc atacattgca tttgattgtt
    49201 cgatgagagc cactcgccaa cctgcttttg ccagcgtgac ggctaatgtt tttccagcct
    49261 tgccaaaacc aataataact gcctgatatt tattcatgat gatttcctgt cgaaacgcgt
    49321 tcatttgaca tcatgactat agacgcaaaa gagcaaatac catttcttca caacgcttga
    49381 tattttgtct tatcgtctgg tattttcttt ttcttaatca gaatcacaaa acaaaaatca
    49441 tgattaacca tttgatggta ttagattagt tatttactaa gattgttggt gtttgtaatc
    49501 aaaaaccact caggagtctg atatggatgc ccttagccgt ttgttgatgc ttaacgctcc
    49561 acaaggaacg atcgataaga attgcgtgtt aggaagtgac tggcagcttc cacatggtgc
    49621 cggggaatta tcggttattc gttggcatgc gttaacgcaa ggagcggcga agctggaaat
    49681 gccgacggga gagattttta cattacgccc gggaaatgtg gtcctgctac cacaaaattc
    49741 agctcatcgc ctgagtcatg tcgataatga atcgacctgt attgtctgcg gcactcttcg
    49801 gttgcaacat tcggcgcgtt attttttaac gtctttgccg gaaacgctgt ttttagcacc
    49861 ggttaaccac agcgtggaat ataactggct gagggaggcg atcccgtttt tacaacagga
    49921 atccagatcg gcgatgccgg gagtggatgc actgtgtagc cagatctgcg ctacattctt
    49981 taccctcgcg gtgcgtgagt ggattgcaca ggttaatact gagaaaaaca ttctcagttt
    50041 gcttctgcat ccacgtcttg gtgcggtaat acagcaaatg ctggaaatgc caggacacgc
    50101 ctggaccgtc gaatcgctgg ccagcatcgc tcacatgtcc cgggcaagtt ttgcccagct
    50161 tttccgtgat gtttccggaa ccacgccgct ggctgtatta acaaagttgc gtctacaaat
    50221 agcggcccag atgttttccc gggaaatgct ccctgttgtg gtgatcgctg agtcagtagg
    50281 ctatgccagt gaatcatctt ttcacaaggc gtttgtccgc gagtttggtt gtactccggg
    50341 agaatatcgg gaaagggtca gacagcttgc accctgaata aaaccgccag aaatcagggc
    50401 aaagataatc cgcattccgg gagttgtgag cctttcaact atttctattt ccaacggtgg
    50461 tttgggcttt atatattttt ttctgatgga ctctagctca gtttttaaat aaaaatgcaa
    50521 actaaattgc ttcaattaaa taaaaccccg actattactt gatacgtgat tattgtcggt
    50581 gattttgttt gtacaccatc agtgtatatc tcaataaccc ctgaataagt agctctgaat
    50641 aggtataagg gatgtagcca tttttaaatg gatttcttat gccaaaaatg atcgtcgatt
    50701 tccctataaa atgtgagcga tgccgaaaga aataaaatta gttattgcat ttgaagtttg
    50761 gatgaaagat catcatttgt catacaaatg aggggtggta tgttgctagc caattaaaaa
    50821 agaacgccat atttattgat gattgatccc ccggtggggg ggaaacatcg ggcataaatg
    50881 ggcatgaagt aatggagtat tagttgtgaa tgtcaatttc tttgtcacct gtattggtga
    50941 cgccctgaaa tcaagaatgg cacgagactc cgtgctgcta ctggaaaaac tcggctgtcg
    51001 cgtaaatttc ccggagaaac agggatgctg cggtcagcct gcgatcaata gtggttatat
    51061 caaagaagcg attccaggga tgaaaaatct gatcgccgca ctggaggata acgatgatcc
    51121 cattatttca ccggctggct cctgcaccta tgccgtaaaa agttacccga tgtatctggc
    51181 ggatgaacct gaatgggcat cacgagccga aaaggttgcc gcgcgtatgc aggatctcac
    51241 ctcttttatt gttaataaat taggggtagt cgatgtaggt gccagtttgc aagggagagc
    51301 ggtgtatcac ccatcttgta gcctggcccg taagctgggg gtgaaggacg aaccacttac
    51361 gctgctgaaa aatgtgcgtg gactggagct gttgaccttt gctgaacagg atacctgctg
    51421 cggatttggc ggcacgttct cggtcaaaat ggccgaaata tccggcgaga tggtgaaaga
    51481 aaaggttgtg cacctgatgg aagtccgccc tgagtattta attggtgctg acgtgagttg
    51541 cctgctgaac atcagtgggc gattacaacg ggaagggcag aaagtcaaag tgatgcatat
    51601 tgctgaagtg ttgatgagcc gctgaggata taaagatgtc gatcaaaacc agtaatacag
    51661 attttaagac acgcatccgt cagcaaattg aagatccgat catgcgcaaa gcggtggcaa
    51721 acgcgcagca gcgtattggg gcaaatcggc aaaaaatggt cgatgaattg gggcactggg
    51781 aggagtggcg cgatcgggcc gcccagatac gtgatcatgt tctgagtaat ctcgacgctt
    51841 atctgtacca gctctcagaa aaagtgacgc aaaacggcgg tcacgtctat tttgcaaaaa
    51901 ctaaagaaga cgccactcgc tacattttac aggttgccca acgcaaaaat gcccggaagg
    51961 tggtgaaatc taaatcgatg gtgaccgaag agattggtgt caatcatgtg ttgcaggatg
    52021 ctggcattca ggtgattgaa accgatctgg gtgaatacat tctccagctg gatcaagatc
    52081 cgccctctca tgttgtggtc ccggcaattc ataaagatcg ccatcagatc cgtcgagtgc
    52141 tacacgaact tctgggctat gaggggccgg aaacgcctga agcgatgacc ttattcatcc
    52201 ggcaaaaaat ccgcgaagat ttcctcagtg ctgaaatagg tattaccggc tgtaatttcg
    52261 cggtggcaga gacaggttcg gtatgcctgg tgaccaatga aggtaatgcg cgaatgtgta
    52321 ccacgctgcc taaaacgcat attgcagtga tgggaatgga gcgtattgcc cccacgtttg
    52381 ccgaggtaga tgtattgatc accatgctgg cgcgcagtgc cgttggtgca cgtttgacgg
    52441 gatacaacac ctggctgaca ggaccgcgcg aagcggggca cgttgatggt cctgaagagt
    52501 ttcatctggt tattgtcgat aacgggcgtt ctgaggtgct ggcctctgaa tttcgggatg
    52561 tgctgcgctg tattcgctgc ggggcttgta tgaatacttg tccggcatat cgccatattg
    52621 gcggtcatgg atatggctct atttatccag ggccaattgg tgcggtgatt tctccgctac
    52681 ttggcggcta taaagatttt aaagatttac cctacgcctg ctctttatgc actgcttgtg
    52741 acagcgtgtg tccggtgcgt attccgctgt caaaactgat tttgcgtcat cgtcgggtga
    52801 tggctgaaaa agggatcacc gcaaaagcag agcaacgggc gataaaaatg ttcgcttatg
    52861 ccaatagtca tccaggattg tggaaagtcg ggatgatggc cggtgctcat gcggcaagct
    52921 ggtttatcaa tggcggcaaa acaccactca aatttggcgc gattagtgac tggatggaag
    52981 cacgcgatct tcctgaagct gacggagaga gtttccgtag ttggtttaag aaacatctgg
    53041 cgcaggagaa aaagaatgga taatcggagc gaatttttga ataacgttgc tcaggcactg
    53101 ggtcgcccgt tgcgacttga accgcaagca gaagatgcgc cgcttaacaa ctatgctaac
    53161 gagcggctta ccgcacttaa ccaacagcag cgctgtgacg cgtttattca gtttgccagc
    53221 gatgttatgt tgacgcgctg tgagctgacc agcgaggcga aggcggcaga agctgcaata
    53281 cgtctgtgta aagagctggg agatcagtcg gtcgtgatta gcggtgacac gaggctagag
    53341 gaattgggga ttagcgaacg tttgcagcag gaatgcaatg ccgttgtttg ggatccggcg
    53401 aaaggtgccg agaatatctc gcaggcagag caggctaaag tgggtgtcgt gtatgctgaa
    53461 tatggtttaa ccgaatcggg aggcgtggtt cttttttccg ccgccgggcg cgggcgttca
    53521 ttgagcctgc tcccggaatc ttctcttttt atcctgcgta aaagcactat cttgccgcgt
    53581 gtagcgcaac tcgcagaaaa attgcatcag aaagcgcagg ccggtgaacg aatgccgtcc
    53641 tgcattaaca tcattagcgg ccctagttca acggcggata ttgagcttat caaagtcgtc
    53701 ggggttcatg gtccggtgaa agcggtgtat ctgattattg aggattgttg agagagatat
    53761 gaagtctgga cataaacctg aagaaggcat acgaggttga aaatagcgtt taggacaacc
    53821 tgacttaacc ccgttccgtc atggttatgc tgctcttctg taaatatcct ggcattagta
    53881 atgccaggat atttacagtg aaaaagacag gcatccattc ctgaaaacgg gttcccttcc
    53941 cggtcattgt cacttcagct ttatacaggc actctctatc agaatgctgt ttattactac
    54001 ccagagtttt gttggtattc atctaccatt tttttgacaa ggcaaaacat tacagaaatt
    54061 acaatgctta gaaaaaatcc cagagccagg caaatggcag cagggtattt attttttatt
    54121 tctggtgagc ttatagttac acagccatca ccgttcggat acaatgacca gttcctggtc
    54181 aggtatatca tttttgtata aagctcactg ttaattgctg aaggtgtttg tatctcatcg
    54241 agaacttgcc tgccattatg gtattctgac gcataccatt tgataataga cgcctgcacg
    54301 cgagatattt ccgcttcagc agttggtaaa tctctcttat ctcctgacca ttccagcttt
    54361 atttctctgt tcttgacatc tgggctggtg tgaaaaaatg tagtaattcg ctgtataaca
    54421 tcttcagtca ttgtggtctg acaggcaaag ttattattgc tatcacagtt aacaagtatg
    54481 cctggtgttg tctggtttat ttttaggctg taactcactt ttatattatc tttattgata
    54541 tacagaaaca acagcaccat tgccatacag atagctataa taaaaaggat aacgcgaatc
    54601 tttttcttca gataaaataa aatctcaatc agatcgatat cctgtttgat ttgttcacgc
    54661 ataatatatc cagaaaataa aatctgtcgc agataaggtt gtattaatag tctgtatcag
    54721 gaatgttcgg ggtaaatatc agcaaaaaac cagcatcatg aatactggat gtttatagcc
    54781 tgcaggcagc agtatgctgc ctgcaggggg tataacagtc gacaggggaa ccgactgctt
    54841 tattcttacc aggtcacatt gataccaact acaccctgta gcgggttctc aatgtcgtca
    54901 cctttggtgt agtcaaggct tgcatatgct cccatgtttt tggtgatatc agcctgtaca
    54961 cctgcaccca cgcggaatgc tgcaccgtca atgctggcat tgacagactc atcacccaga
    55021 cggactttgt tgccatcaga gaattcgttc agtgcggcca gattcagata aggtttaacg
    55081 gtcgagttgc ccacagcgaa gtcataaccc acgcgcgtac cggcctcacc cagcacagag
    55141 ttatagctgt cagaatgaac gtccacgccg ttgagctgat aatccacacc ttccagcgtc
    55201 agcgcagaca gtttggcata tggctgaata aacaggccat taccgtcttt ccatgtgtaa
    55261 ccgcctttaa ccatcgcacc gaaaccattg gtgttaaagt cagctttgat ggtaccgcca
    55321 ccattcatca ggcgaacatc tgccgtgttg ctgtagtgac caaactgtgc agcagtatca
    55381 atgaagatac cgttgttata ctggcggctc aggtaagcgt ggaagctgta accttcagtg
    55441 tcgcctttgc tctgcatggt ggtcatgtca cctttcgcag aagacatcgc tacacctgcc
    55501 agccagctac catcttcagt catgaagcgg gtatcaccac ccagcattac accgtttaca
    55561 tccaggtcat aggatgcatt tcccgcggtg gtatgtttct gtttaccgcc aaagtactga
    55621 atccatacgc cgcccttgtc attttcgctc agacggacag catcctgacg ggctgagagc
    55681 gtgtcagcct gcagattagc aacgttagcc ggagctgctg ccattgcctg aatcagggag
    55741 ctggcgttgg atgctttatg cgtgtttttt acgaaataaa agttaccatc agctccctgt
    55801 gtcaggtcat attgatacag accaccttcc gttgtgcctg tggcttttag agtgctgtct
    55861 ttgccggtag aaataaatgc cgtttgttca ttgataagat ccaatgaacc cgcactgtaa
    55921 acacgcaagt caccgctcgc ttttccgctg atatccagag tgttttcaga agaggcatca
    55981 acaatcagag tgccgttctg agaagcaaac gtgccagtat tgactgtagc cttcgtgata
    56041 ttaaccgtac tgtcttttac tgccagagca tcaacagttg aggatcccgt aacattccag
    56101 acagacccgt tttgcagaga tacactggca tctcctgccc ctgaaacttt gcctttccag
    56161 actgagctat cggaaagatt tatatccaga ttactacccg ctttatcgcc gctattgaag
    56221 gtataagcat taccttccca gtaagagcgg gcaacgttca tgtttacatt tgttgtggta
    56281 ccggctgaat tctcgcttgc agaaagggca tggatatcag agtagatggt tgaatcggcg
    56341 acagataaat ttaaattttg cgttgctgaa cctgctcctt gtgccgaagc agaagcataa
    56401 attgtttgtt tattattagc cgccccaatt actgagccat ccttaatatt cagattgact
    56461 gtatgagttc ccgtattctc cttggcaata actattgttt caatccggcc attaataata
    56521 tttgagccag caagggtaat gttgtcggtt gcatcacctt taaaaccatt caatacgcta
    56581 cccttttgag caaaaattgc cgtagagccg ttgctggaac tcttattacc ttgagcattt
    56641 atggttgtac catttagcgt tacattattt cccgtggccc cttccagaga tattcctgtc
    56701 gcactacttt tttcattttt tgcaacgaaa ttaccgccat taacaacaac atcgccagtt
    56761 gaagaaatga ccattgcagt ccggacatag gtgccttcac cttgagctgt gatggtggag
    56821 cctgtagcat taatcacaac atttttgtca ccgctaactt ttacaccttc aaggaatttt
    56881 ttgtctgccg ttgattcagc agtaatatca tgaccgccaa aagtaacatt aagatcttta
    56941 tctgccgcat taattgttac ggctgtaatc tgttggtctg ctgtattgct aataacaaca
    57001 tcttgatcca gagttgtctt atctttatcc agtgtaaccg gtgtagttga taccgcgtta
    57061 tcagcagcga atgctggtgc agaaagagcc ataacaatag ctgtcgctaa tgcgctgcgg
    57121 taaaatgcct tactgttttt catgaagaaa ttctccattc tggtaactaa tttcaatttc
    57181 ccacttattt atatttatgc agagataaag cctgcgtccc tgcatttacc accacggaat
    57241 acctgttggt atttccgttt tcacgcaatg tcatggcatt cgttttttcc aatgcatttc
    57301 catcaccgtg aaatttattg ctgctcagca gcagggcttc ccgcaatgac atgctgtccg
    57361 gtattcccca acgctttttt agcgactgct cagcaagcca tatctgtctt atccccgttt
    57421 tgagaaactg cgcactgtat tcccagctgt gtccagcaag aagcaacgac aacaccttct
    57481 tttgcttttc cgggaagcgt tctgacatga cgattgtctt tgtgcggtta ttcacctcat
    57541 tcataaagac agagtaactg tccataactg gcaaattttc tgcatgtttc agtcctgaaa
    57601 acccaccaga ggcatttgca acactgatag cacgctctac tgacattcct gttttcagta
    57661 acctcaggtt tttgagaacg ccagaaatac tcacatccgg tggcagaaaa aagggcgcga
    57721 cgcacaacac caccacgaat gagtgatccc gataaaatcg gaaccacatt tcagcgcatt
    57781 cggcaggtcg ccctgccgat atcacgacac tctgcggcct gtgccgcaat ttattgcgta
    57841 actcttcttc tgttctgatg caggtaatat tgcagacaga accggttgat agagcttcaa
    57901 actgtgcagg tacaattcct gagggacaga aaagaacgaa acgcatcctt ccctcacaga
    57961 tgtcagtacg ccagcacgct gtacaggctg tacagcgtgc ctcccactcc tgcagcccag
    58021 agcagtagaa atattttggc ccctacactg tagcgagtcc gtacgcgccg cagtgggtgg
    58081 tagtaacaac gctgctgtgt cataacactc ctggtctttt tttagacata tgttttcttt
    58141 gcccggaaag cacttttccg gatatggcgt cagaatttat tgatttacca acgataactt
    58201 tctctggata aagaattccg gacaccatgt tctgcacata ctgagtgagt cgatgagcgg
    58261 aatgttgtcc gacatatctt gcatgtagcc gtatattccg tttgactctg ctgtcaccgc
    58321 atcctgtata gagcagcctg aaatcgctta gtgttcccag tccaagtttc cttaatgcct
    58381 gtgtggcaat cgcatgcaga gtctttgaac tgcgttgttc ataacgggca atctcactga
    58441 aactcatgcc ctgcatcaga atcttaagca cgcttaactc ccgtgcagtc aggagccaga
    58501 aatcactcat atcgccgggt aaaggacgta gtaaccagcg gataatttga ttctgataac
    58561 gactcgcttt cagcaaggtg ccgatatgtg ggaagccgtt atacagactt gaagcaggag
    58621 cattatcgaa ccagtcggag tcaccggtca ggcagacttg ccattgcccg gcatcactcc
    58681 gcaagcggac aagacaagcc agaagaacta actcccccga aggaagcgaa gggatatcag
    58741 ccaacaggta gccacaggag gtctttaaaa cgaattcaac ctctgtagtg acgttgctgt
    58801 acacccgcac gatatacctg tccgctggga tgagagactc cagtgccagc ctggtgaact
    58861 gacaggcact gataataacc agaggcttct tcattcagac tcgtccacgc cattgctaca
    58921 aaacctgaca ttttgattca aaatgtcagg tttggtttaa tagcctgaat atttaatttt
    58981 cttggaatat aaatctaatt tgtttgattt ttattgtttt taattttgtg ttatttaaca
    59041 atatgagcga aagttaatca ctggtatgat ctttgtggtt aatcatttca aatcatgggg
    59101 ttattttact ttagcgagaa atatcaacat gttatgtgtt ttatttaatt gaatgggctg
    59161 tttttttatg gaagaattga ggaaaacgta ggaatctgac attttgaatc agaaggtact
    59221 gttttaacga ggcttttttt tccacacccc tttaaaacga gcagcattac tggctgtgta
    59281 tctgacagta tgctgaatat ttcgatgacc cagataatcc tgaagtagtc gggtatcgac
    59341 accattgtca gccagagcaa aaccacaggc atgacgtaac atatgaggat gtgcttttac
    59401 agctaatcct gcacgttttc cagcctcacg aatgatagaa aaaaaatgtt gtctgctgat
    59461 agggcggcgt ttccgggaga gaaaaagcca gtctccttca actccgtttt caattaactt
    59521 cctctgctta agccacaact tgatcaaatt atattcatca ggaagaagag gatgtgttgt
    59581 cgagaaacca tttttaatac gccgaatatt aagttgtttt ccagatgcat caatatcgga
    59641 taaacgtaaa tcaagaagtt cactggccct aaaaccgtga atgaaagcca tcataattag
    59701 acagcgattt ctttcaggaa aggacatact ctgggctgca tccatcagcc tgtagacttc
    59761 atcctgtgtg agatattttc tcgtcatacg ccaccgtgta cagttttaaa aaaaagagag
    59821 gcacacccag ataagtatgc ctgcaatcag aacgttatcg atgtaatctc attatctcat
    59881 tattaagatt ttttgaatag tagttctacg caacagatta aatatgaact atttattgtg
    59941 cagcttttct acaagtcatt tttaatgcgt gtttttatct acagtgatgt gagaaaataa
    60001 atataaaagc tatgaactac agggcgtggg ggatgtgtgt tacgtctaat cttttagtga
    60061 tgtttagtag ttttgagata tttgacgtta tttcttatga ggttgttttt attattatct
    60121 tattgatttt atatgcattt tgtcgttctg tgaagttagg gtgtagaaaa taaaaattta
    60181 atattattcc cgttaattta caataacaca ttgttgtaag atatttttta gtcgtaagta
    60241 aatcattatt gatgggttct gctaataaat taaagatgtt ctcttaaata gtttttttgc
    60301 tttttgtaaa tatcaatttg tttatttttg attcatttgc tggagagtat ttcaacaatt
    60361 tttatttttc gcataaactt attaacaact ttttattatc aattgaaaat taatgtaaaa
    60421 taaaatgtta gcgaagcgat aatcttgtgc taaaaaatat ttaagcatga tgattaatta
    60481 aagaagagta aaaataaatc actgccaata aattatacga atcgtgttta aagctccacg
    60541 ttgttttggt ctgacttatt tttgtcctcg tttcaagaac ccttttcgta tatgctaacc
    60601 gtgagcggga gtgaacaagt caccatagcc aggaacacta gcggcgtaat tcttaagctg
    60661 agtggcattt cacctgcggt ttatcagtgc attcatagca ccttcatcag ccagattgaa
    60721 cgagtccgct tagaatttgc tggcgaggtt gtcactatga agcatgagag ttacccctat
    60781 gttttatata acgattctgt ccccatacct ggaacggtaa ctctcaatca gatcggttct
    60841 tgagtcagtt caaatcacga ctcatttttt cgctctcacc ggcatcccat ttgccacaaa
    60901 atatcccgcc gtgctcctcg gcagcgcttc ctgtccacga atcatatccg ctattttctc
    60961 gccaatcata attgtcgtgg cgttcaaatt cccggtgata atctgcggca taatcgacgc
    61021 atccaccaca cgcaggcctt ctaacccgtg tacgcggcct tcgccgtcaa ccacggacat
    61081 ctcgtcgtaa cccattttgc aggtaccgca cggatggaag gcggtttcgg cgtggttacg
    61141 cacgaactca tcgagctgtt catccgtctg acattccgtg ccggggctga tttcgcggcc
    61201 acgatactga tccagcgcgg gttgatgcat gatctcgcgg gtgatgcgaa ttgcgtcgcg
    61261 gaactcctgc cagtcctgct cgtgcgacat gtagttaaac agaatcgccg gatgctggtg
    61321 cgggtcgcgg gatttaatcc gcacatgccc acggcttggc gagcgcattg agccgacgtg
    61381 gcactggaaa ccgtgctctt tcactgcatt cgagccgtta tagttaatcg ctactggcag
    61441 gaaatggtac tgaatattcg gccacgcaaa ttcctcacgg ctgcgaataa atccacctgc
    61501 ttcaaagtgg ttgctggcac caacgccagt gccgccaaac agccactccg caccgatttt
    61561 cggttggttc caccactgca gtgcagggta gagggaaacc ggttctttgc actcatattg
    61621 cagatacatc tccagatgat cctgaagatt ttcgccgacg ccgggtaatt catgcaccag
    61681 cggaatatca aactccgcca gcagttcagc gttgccgacg ccggagcgtt gcaggatctg
    61741 cggtgaggca atcgcgcctg cacataacag cacttctttg ttggccgttg cgcgggttgg
    61801 gatggtgctg tcgccttcca gccattcgac gcccaccgcg cgtttgccgt caaaaatgat
    61861 gtgatcggtc atagcgtgag tacgaatggt caggttaggg cgcgatttgg cctgatcgag
    61921 ataaccgcgc gcggtactgg cgcgacggcc ctgcggcgtg acggtgcgat ccatcgggcc
    61981 aaaaccttcc tgctgatagc cgttgagatc gtccgtgcgc gggtagcctg cctgcacgcc
    62041 cgcttcaatc atcgcttcaa acagcggatt gacgccgggt ttggaggtgg tgacgctcac
    62101 cgggccatcg ccaccgtggt agtcgttctc gcccacatcg cgagtctcgg ccttgcggta
    62161 gtagggcagg cagtcgagat aactccagtt ctccagaccg ggttcttgcg cccagttatc
    62221 gagatccagc gcattgccac ggatgtagca catgccgttg atcagcgacg atccacccag
    62281 gcctttaccg cgtccgcact ccatgcggcg gttattcata aacggttcag gctctgtttc
    62341 ataggcccag ttgtagcgtt tgccctgtag tgggaacgcc agcgctgcgg gcatctgggt
    62401 gcggaagtca aagcgatagt ccgggccgcc cgcttcaagc agcagcacgg aggtattcgg
    62461 atcttcagtc agacgggtag cgagaacgtt gccggctgag ccggcaccaa taatgatgta
    62521 gtcaaattgc aaataaacct cctggttaga atatggactg gaatttagcc atctcaacct
    62581 ggatggactt cacctgggtg taactctgga gcgtcatcac gccgttctcg cgaccaatgc
    62641 cggagtgttt gtagccgcca acgggcatct ctgccgggga ttcgccccag gtgttgatcc
    62701 agcaaatacc cgcttccagc tgatgaatga cgcgatgcgc gcggttcagg tccgccgtca
    62761 cgatgcccgc cgccaggccg tagtcggtat cattggcgcg gcgaatcact tcgtcctcag
    62821 attcataagt gagaagcgac atcactggcc cgaagatctc ttcacgcacg atggtcatat
    62881 cgtcgctgca atcggtgaac actgtcggtg caacccatgc gccgttatcg aagccatcgc
    62941 ctttcagtac atcgccgccg cacagtacgc gcgcgccttc ctctttgcct ttggcgatat
    63001 agcgcagcac gttatcgcga tgcgggaagc tgaccagcgg gccgaagtta gtttgcggat
    63061 cgaaaacgtc gcccgcgcga atgcgctcaa cgcgcgccag aattttctgc tcaaatgcgg
    63121 ctttgcattt cgccggaacg aagacgcggg tgccattggt acacacctga ccggagctga
    63181 agaagtttgc catcatggcg atatcggcgg cgagatcgag atccgcatca tcgaaaacga
    63241 tcagcggtga tttaccgccc agttccatgg tcacttcttt cagggaagag gccgccgagt
    63301 tagccatcac ttttttgccg ctggcgacac cgccggtaaa tgacactttg gcaatgcccg
    63361 gatgctcggt cagatattgc ccggtctccg cgcccacgcc cggcaacacg ttaaatacgc
    63421 cgtccggcag gcccgcttcg ctgtaaattt cagccagctt taacgcggta agcggggtaa
    63481 cttcgctcgg tttgaaaatc attgcgttgc ctgccgccag cgccggggcg gatttccaca
    63541 gggcaatctg gatcgggtag ttccatgcgc caatccctgc cactacgccc agcggttcgc
    63601 ggcgggtata cacaaaggac gtttcacgca acgggatctg gctgccttcc agcgccggga
    63661 tcagcccggc gtagtactcc agcacgtccg caccggtaac gatatcgacg gttgaggttt
    63721 ccgaatatgc ttttccggtg tcgagggttt ccagttttgc gagttcgtca ttgcgttcac
    63781 gcagaatatc aacggcccga cgcagaatac gcgagcgctc catagcggtc atcgccgccc
    63841 agattttttg cccctgttgg gcactcttca cggcgcgatc gacatcctca cgcccggcgg
    63901 cctgcacggt cgccagcacg ttaccgttgg ccgggttaat ggtctcgaag gtgcgaccgc
    63961 tggtggcgga ggtataacca ccatgtatat aaagctgctg ttctgccatt cgggacatca
    64021 cgtctcctcg gttaatcggt gggtaaatgc tgagtaataa agtgacgggt cagcgagtgt
    64081 gcgagaggtt tatccagcgg tttgccgctc agtgccgcgc gcagccataa tccgtcaatc
    64141 agcgcggcaa ggccgtagcc cgcttcctgt gcctgttggc gcggtaactc gcggcgaaat
    64201 tcgctcacca gattcgacaa taagcggcgg ctactgacct gctgtaaacg atagagcatc
    64261 ggctgatgca tactgctggc ccagaacgcc agccaggctt tcatcgccgc actgctcacc
    64321 tgcgtttcat cgaagtttcc gccaacaatc gcctgtaatc gctgctctgc actgccctgc
    64381 ggaagtgcat gtaatcgatt caaaaccgcg tcacgcagct gactggtgat atcgcgcatg
    64441 gttgcttcca gcagaccatt tttgtccctg aaatagtggc tgatgatccc cgtagaaacg
    64501 cctgcacggc gggcgatctg cgcgatcgtt gcatcgtgca tgcccacttc atttattgct
    64561 tccagtgtgg cgtcgatcag ttgtctgcgc cggatcgact gcatccccaa tttgggcatt
    64621 ttcgccactc cattcatcag cgttgtttat ctattaaagc ggttattgat tggacgttca
    64681 atataaaatg tgtcttaatt gttacgaatt tgattttaaa tggtaacaat aacagtgggg
    64741 atactggatg acagaccttt cacacagcag ggaaaaggac aaaatcaatc cggtggtgtt
    64801 ttacacctcc gccggactga ttttgttgtt ttccctgaca acgatcctgt ttcgcgactt
    64861 ctcggccctg tggattggcc gcacgctgga ctgggtttct aaaaccttcg gttggtacta
    64921 tctactggcg gcaacgctct atattgtctt tgtggtctgt atcgcttgtt cgcgttttgg
    64981 ttcggtgaag ctcgggccag aacaatccaa accggaattc agcctgctga gttgggcggc
    65041 gatgctgttt gctgccggga tcggtatcga cctgatgttc ttctccgtag ccgaaccggt
    65101 aacgcagtat atgcagccgc cggaaggcgc gggacagacg attgaggccg cgcgtcaggc
    65161 gatggtctgg acgctgtttc actacggctt aaccggctgg tcgatgtatg cgctgatggg
    65221 catggcgctc ggatacttta gctatcgtta taatttgccg ctcaccatcc gctcggcgct
    65281 gtacccgatc ttcggtaaac ggattaacgg gccgataggg cactcagtgg atattgcagc
    65341 ggtgatcggc accatcttcg gtattgccac tacgctcggt atcggtgtgg tgcagcttaa
    65401 ctatggcttg agcgtactgt ttgatattcc cgattcgatg gcggcaaaag cggcactgat
    65461 cgccttgtcg gtgataatcg ccacgatctc tgtcacctcc ggtgtcgata aggggattcg
    65521 tgtgttatcg gaacttaacg ttgcgctggc gctgggattg atcctgttcg tattgtttat
    65581 gggcgacacc tcgttcctgc ttaatgcgct ggtgctgaat gttggcgact atgtgaatcg
    65641 ctttatgggc atgacgctca acagttttgc cttcgaccgt ccagttgagt ggatgaataa
    65701 ctggacgctc ttcttctggg catggtgggt ggcatggtcg ccgtttgtcg gcttgttcct
    65761 ggcgcgtatc tcgcgtgggc gtaccattcg ccagttcgtg ctgggcacgt tgattattcc
    65821 gtttaccttc acgctgttat ggctctcggt gttcggcaat agcgcgctgt atgaaatcat
    65881 ccacggcggc gcggcatttg ccgaggaagc gatggtccat ccggagcgcg gcttctacag
    65941 cctgctggcg cagtatccgg cgtttacctt tagcgcctcc gtcgccacca ttactggcct
    66001 gctgttttat gtgacctcgg cggactccgg tgcgctggtg ctggggaatt tcacctcgca
    66061 gcttaaagat atcaacagcg acgcccccgg ctggctgcgc gtcttctggt cggtggcgat
    66121 tggcctgctg acgctcggca tgctgatgac caacgggata tccgcgctgc aaaacaccac
    66181 ggtgattatg gggctgccgt tcagctttgt gatcttcttc gtgatggcgg ggttgtataa
    66241 atctctgaag gtagaagatt accgccgtga aagtgccaac cgcgataccg cgccgcgacc
    66301 gctggggctt caggatcgcc tgagctggaa aaaacgtctc tcgcgcctga tgaattatcc
    66361 gggcacgcgt tacactaaac agatgatgga gacggtctgt tacccggcaa tggaagaagt
    66421 ggcgcaggag ctgcggttgc gcggcgcgta cgtggagcta aaaagcctgc cgccggaaga
    66481 gggacaacag ttggggcatc tggatttgtt ggtgcatatg ggcgaagaac aaaactttgt
    66541 ctatcagatt tggccgcagc aatattcggt gccgggcttt acctaccgcg cacgtagcgg
    66601 taaatcgacc tactaccggc tggaaacctt cctgttagaa ggcagccagg gcaacgacct
    66661 gatggactac agcaaagagc aggtgatcac cgatattctt gaccagtacg agcggcacct
    66721 taactttatt catctccatc gtgaagcgcc gggccatagc gtgatgttcc cggacgcgtg
    66781 attgttattg cataaaaccg cgccatgtct gcatatggcg cgggaatcat tgactcgctg
    66841 taaaaaatta tgcatatatt atatatgatt gtcatgtcat atcctgaaat tcctcttgta
    66901 tcacttcatg attttatatt ttattaatta acgattttat tttgtattag ctgaatggat
    66961 tcagtcctat agggttatat atttattaat aagacgattc ttatatttgg atacctaata
    67021 agagttacca cttttaccgg ctgtatatta aatgtatttc tcctgctttt gtttatattt
    67081 tgagttgatt tgagttcctg gatacatata atgcgcaggt attagagact ggggggggaa
    67141 tgtaatgata aagttaagag tgttaactta aaataagttt tcagtagtaa cttgctattt
    67201 tattaccctc tgaactacat actcagatat gtggttgtca atgctatgca aaatttttga
    67261 atttttatat gcgatgaaca gtaaccggaa tggcatttaa tgcgttgctg tttatgcaat
    67321 cttggtttta tttggatgta ttgctggaga ttgtgatgaa taaaatatat cggctaaagt
    67381 ggaacaggtc ccgtaactgt tggagcgtct gctcggagct ggggagcaga gtaaaaggaa
    67441 aaaagtcccg ggctgtttta attagcgcga taagtttata ttcatctctg gtattcgccg
    67501 atgatgtcat cgtaaaccag gataaaacta ttgattttgg caaagagaac cagagcatcg
    67561 attaccgtat tacggtgact gacaatgcca atctggtaat caatgcgaca gatacttccc
    67621 gtccgcgtct gactctcgct tctggtggtg ggttggatat taccggagga aaggtaacaa
    67681 tcaatggccc acttaacttt ttgttaaaag ggacggggtt cctgaatgtc tccaatgctg
    67741 gcagcgagtt atatgctgat gatttgtatg aatcaaactc aggcatgaga cacgatcgcg
    67801 gctattttaa tgtttccaac ggcggcaaaa tccatgttaa gggcaccagc cgtctgacct
    67861 atttgcaggg aaatgtcagt ggtgaaggca gccaggtaaa ttctaaaacc ttctttatgg
    67921 gcgtttacgg cagttacggt ggtaatcagt acctgtcagt taataacggc ggtgaagtta
    67981 atgccagtga gaaaattagc ctgggttatt atgatcaaaa ctccgataca acacttgctg
    68041 tttcggaagg tggtaaaatc tctgcgccga ctattagttt aagcaccaac tctgagttag
    68101 cgttaggtgc acaggaagga agtgctgcga aggctgcagg gattattgat gccgaaaaaa
    68161 ttgagtttgt gtgggcaaag acatccgaga agaaaatcac cttaaaccac acggataaaa
    68221 acgcgactat ttccgcggat attgtcagtg acagcgaggg cctgggctat atcaatgcgc
    68281 tcaatggtac gacttactta accggggata actctgcctt tagcggcaaa gtaacgattg
    68341 agaaaaatgg cgctttgggg atcacccaaa atataggctc tgcagaaatc accaaccgcg
    68401 ggaaattaca cctgaaggct gaggatagca tgacctttgc caataagatc tccggcaacg
    68461 gtacaataag tatcgacagt gggacggtgg agttgaccgg caataactat gcattctacg
    68521 gatatattga tgttgcttcc gatgctgtag ctgttatttc tgaagacaag aacatcggac
    68581 gtgcggaact ggatgttgat ggcaaactgc aaatcaatgc caataaagat tgggtatttg
    68641 ataacgatct tgaaggtaga ggcattgttg aaataaacat ggggaatcac gaattctcct
    68701 ttgatgagtt tgcttataca gactggttcc agggttcact ggcgttccag aacacgacat
    68761 ttaatctgga aaagaatgct gagtttctgc agaaaggcgg gatcactgcg ggtcagggaa
    68821 gcctggtaac agtgggtaag ggcgctcact ccattagcac tttgggattc tccggcggaa
    68881 cggttgattt tggtgccctg gcagcaggtg cacagatgac agaagggaca gtcaacgtta
    68941 gtaaaacgct ggatttgcgc ggcgagggtg tgattcaggt ttctgacagt gacgttgtcc
    69001 gctcagtatc tcgtgatatt gactccgcgt tatcgcttac tgaagttgat gatggtaaca
    69061 gcaccattaa gttggttgat gcgcaaggtg cggaagttct gggcgatgcg ggcaatctgc
    69121 aattgcagga taaaaatggg caaatcctct ccagcagcgc ccaacgtgat attcagcaga
    69181 atgggcaaaa agcggccgtc ggcacttacg actatcgtct gacgagtggg gtaaacaatg
    69241 acggtctgta tatcggttac ggcctgaccc agcttgattt acacgctacc gacagcgatg
    69301 ctctggtgct gagctctaac ggtaaaagcg agaatgccgc cgatctcagc gcaaagatta
    69361 gcggcagtgg tgacctggca ttcagcagcc agaaaggtca gaccgtatcg ctttctaaca
    69421 aagacaacga ctataccggt gttaccgatc tgcgcagtgg gacgcttttg ttgaataacg
    69481 ataacgtgtt gggtaatacc catgaactgc gtctggcggc agagactgaa ctggatatga
    69541 atggtcacag ccagaccgtg ggcacgctca atggcagcgc cgattcactg ctgagcttaa
    69601 atggcggcag tctgacggtt accaacgggg gcacttcaac cggttcgtta acggggagcg
    69661 gagagctgaa tattcagggc ggcacgctgg acatcgcggg cgataacagc aacctgacgg
    69721 cgaatgtgaa cattgctaat tcggctaatg tcctggtaag tcatgcgcag ggattgggta
    69781 gcgcaaacgt tgagaacaac ggtaccctgg cgttgaataa tagcactgaa aaaagagcgg
    69841 ctgcgtctgt gaattacacc ctgggcggca atctgaccaa caacggtacg ctgatgaccg
    69901 gaatgtcagg acagcaagct ggcaatgtgt tagtggtgaa ggggaactac cacggtaata
    69961 acggtcaact agtaatgaat acggtactga atggcgatga ctcagtaacc gataaattgg
    70021 ttgtcgaggg cgatactagc ggcacgactg ccgttacggt gaataacgct ggcggtatag
    70081 gtgcgaaaac ccttaacggt atcgaactta tccatgtaga cggtaagtct gagggcgaat
    70141 ttgttcaggc tggtcgtatc gttgcggggg cgtatgacta cactctcgcg cgtggacaag
    70201 gggcaaatag tggtaactgg tatctgacca gcggcagtga ttctcctgaa ctgcagccgg
    70261 agccagaccc gatgccgaat ccggagccaa acccgaatcc agagccgaac cctaacccga
    70321 cacctacgcc gggtccggat ctgaatgtgg ataatgacct gcgaccggag gcgggtagct
    70381 acattgcgaa ccttgcagca gcgaatacaa tgttcaccac gcgtctgcat gagcgtctgg
    70441 gtaatacgta ctataccgac atggtgacgg gtgagcagaa acaaaccact atgtggatgc
    70501 gccatgaagg tggtcgtaat aaatggcgtg atggcagcgg ccagctgaaa acccaaagca
    70561 atcgctatgt tctgcaactg ggaggcgatg tcgcgcagtg gagccaaaac ggcagcgaca
    70621 gctggcatgt tggggtcatg gcgggatatg gcaacagcga cagcaaaacc atttcctcgc
    70681 gaaccggtta tcgtgcaaaa gcgagtgtga acggatatag caccggcctc tatgccacct
    70741 ggtatgccga tgacgagtcg cgtaatggcg cgtatctcga cagttgggcg cagtacagct
    70801 ggtttgataa cacagtgaaa ggggatgact tacaaagtga atcctataaa tcaaaaggat
    70861 ttaccgcttc actggaagct ggatacaaac acaaattagc tgaatttaat ggcagccagg
    70921 gaacgcgtaa tgaatggtat gttcagccgc aagcacaggt tacctggatg ggagtcaaag
    70981 ccgataagca ccgcgaaagc aacggaaccc tcgttcatag caacggtgat ggcaatgttc
    71041 aaacccgact tggcgtaaaa acctggctga agagccacca taaaatggat gacggtaaat
    71101 cccgcgagtt ccagccgttt gtagaagtga actggctgca taacagtaag gatttcagca
    71161 ccagtatgga tggcgtgtcc gtcactcagg atggagcccg aaatattgct gagataaaaa
    71221 ccggggtgga aggacagcta aatgccaacc tgaatgtctg ggggaatgtg ggcgttcagg
    71281 ttgccgatag gggatataat gacacctctg caatggttgg cattaagtgg caattctgat
    71341 ctgaataatg agtaaaagga gagagagccg aacgggagat aattcggctc tctgtgtcat
    71401 tttcttgcag ccagctttat cagtcagagt gaagactcgc agatttagct ggcgcacaag
    71461 atttaatcaa ggtgtgttca atggcttcgc atgatttgag tgtttttttg gaagagttcg
    71521 gggcaacggt gaatttgacg ttgcctggta tcgtgtccga aaaagaacgg cttttactca
    71581 agctgctaat ggagggaatg tcggttacag aaatctcaca gtacagaaat cgcagtgcaa
    71641 agacaatttc acatcaaaag aagcagctgt atgaaaaact aggcatccag agtgatatta
    71701 ccttctggcg ggatatcttt tttcaatatc atccgcaagt catcagtggc acgggaaata
    71761 aaaataattt ttatatccct gataaccgtt gtcaccatat cgtcacgcct gaagccatca
    71821 gtctggcgtt ggaaaaccac gaattcaaac cgtggatcca accggttttc tgcgcgcaga
    71881 ctggggtact gacgggctgt gaggtacttg tccgctggga acatccacaa acgggaatta
    71941 tcccaccgga tcagtttatt cctctggcgg agtcatccgg tcttattgtc ataatgaccc
    72001 gccaactgat gaaacagact gcggatattc tgatgccggt aaaacatttg ctgccggaca
    72061 atttccatat tggcatcaac gtctcggcgg gttgtttttt ggcagcggga tttgaaaaag
    72121 agtgtctgaa cctggttaag aaattaggta acgataaaat caagctggtt ctcgagctaa
    72181 cggaacgtaa tcctattccg gtaacgccag aagccagagc gatatttgac agccttcatc
    72241 agcacaacat tacctttgcg ctggatgact ttggtacggg ttatgcgacc tatcgttact
    72301 tgcaggcgtt cccggtcgat tttattaaga tcgataagtc atttgtgcaa atggcgagtg
    72361 tcgacgaaat ctccggtcat attgtggaca atattgtcga actagcgcgt aagcctggtc
    72421 tgagtatcgt ggcggaaggg gtagaaaccc aggagcaggc ggatttaatg atcggtaaag
    72481 gcgttcactt tttgcagggc tatttgtact ctccgccagt accgggtaat aaatttatct
    72541 ctgaatgggt aatgaaagca ggtggttgat gtaaaccgct attcacagcg catcgggagg
    72601 ttggcagcga ttaattctcc cgatgcagta aatcctgata gatccccgtt aatatgccat
    72661 ttggcgcaaa ttgtttttta atccactgcg tcacctgacc cgtagcagaa tgctgagtcg
    72721 ccagtaacat gcgcgaatcc tggcgcggat tatggatttg tcgggtaacc agcagggatt
    72781 gcgtcatcgc ctcacggacc atgtaatccg gcaaaaaacc aattccttca cctaaaatct
    72841 ggcactgaca tttggtgttg aaatctggca ccagaattga ctcctgcccg tgcagcaacc
    72901 agcccacttt tttattaatc gtatgcgcgg tgtcctccac cataatatta gggtagagac
    72961 gcagttggct ttcggcgatg ggttccggca taaaggctaa cgggtgatcc ggggcgatag
    73021 caaaagccca gcgaatcgcg ccaatttcag tgtaatcaat accgccgccg tccagtagtg
    73081 tatcgggtgc gccgatggcg atattggcct gattattaat aatcgcatcc cagacgccgt
    73141 tatacacttc ggtcgtcacg gtaatctggc aggtaggaaa ctgctttttc agcacctgta
    73201 ataagcgcgc ggtgtgtttg ggcgtataaa gcagctgatt aatacaaatg cgcacccgtg
    73261 cttctatgcc ctgcgaaata gtatcgatac tgcgtttgat ggcgtgaaaa tcattcagca
    73321 ggtcggtagc tttgcggaaa aaataacgcc cggactcggt taactcaatg ctgcgggtgt
    73381 tgcgagtgaa cagcaccaca tccagccccg tctccatacg cttgatggtg tagctaatgg
    73441 cggaagtggt taaacccaac tcgtctgcgg ctttactgaa gctgccaaaa cgcgcagcgg
    73501 tagtaaatgc caacagattc tcttcggtaa aaattgagtt cattaaacca ttctcgccat
    73561 tatctactat gaatttctcc agctatgact gtagaggtat cggttaaaga tagataatca
    73621 tttttgaata acttttaata cccgtcgcgt ttagtcgtcg cttcgcttaa tttttgtcca
    73681 ccatattctg aaattatccc cacgcagtta cccgccagaa aacccagtaa cgtcaccgtc
    73741 cagttcatcc cgctggcaaa aaacagcgtc atgccgagaa agccaccggg aataaaggac
    73801 agtaaccaga aacgcccttg ccagaccact accgccgaga agggtacggt agtaattacg
    73861 ctcgcccaga acgcggacag tccacttaca gaggccagcc aaccggcagc cagcgcggcg
    73921 aaaaatgccc acaccatccc ggaataatta accgccagac ttctgataaa ccccgattta
    73981 ccgccgcctg cggcataaaa actgcaaaag gcgacgaagc caatggttcc taataattcc
    74041 catccctgag agagcccgac atgtgaagac actaactgcc aaagccctgc acaaataccg
    74101 acggtgacac ccgttgccgt gagtccgttc atcttgttcc cctctggtta gacatggacg
    74161 aaagctactc ttacatgtgc agactataga gaaataaaac ctgattgagt gaaagggtat
    74221 cgggtcaaag aaacaaatat tgcactaccg cacactgcga aaagattgtt gaacatcatt
    74281 caacaaaaag gcgaaataca gcgaattaaa tttaagcggg ttcttttatc agattatcgt
    74341 tgctattctc aattcatcga aaacagccac aaaaaccagg ggaatgtgaa tgagtataaa
    74401 aaatctacct gccgactatt tattggctgc acaacagggt gatatcgata aagtaaaaac
    74461 ctgccttgcg ctgggtgtcg atattaatac ctgcgatcgt caggggaaaa cggcaattac
    74521 gctggcaagt ttatatcagc aatatgcttg cgttcaggca ttaattgatg ccggagcgga
    74581 tattaataag caagatcata cctgtttaaa tcctttttta attagctgtc tgaacgatga
    74641 tttaacgcta ctacgaatta ttttaccggc taaacccgat cttaattgcg taacccgttt
    74701 tggcggtgtc ggcctgacgc ctgcctgtga aaaaggccat ttaagtattg taaaagagct
    74761 tttggcgcat acggagatta acgttaacca gaccaaccat gtaggctgga cgccgctact
    74821 ggaagcgatt gtgcttaatg atggtggtat taaacagcag gcgattgtgc agttattgct
    74881 ggaacacggt gccagcccgc atctgaccga taaatatggc aaaacgccac tggaactggc
    74941 gcgggaacgg ggctttgaag agattgcgca gttactgatt gccgcaggtg cataaaccgg
    75001 gaggcttgct atcaacacac cagaaagacg gtgtgtgtgg gcgctaactg cggatgcgga
    75061 ttttctggcg cagcgggggc aaggacaggt tgaacaggtc tttgccagag cggtaaatat
    75121 cgcactcccg gctcgccagc agttgctgac gctgctttgt gaagagtacg acaatgcgcc
    75181 aaacagttgt cggttggcac tcactcactt tgatgatctg ttccggcatg gtgataaggt
    75241 tcagtttgac gatcaaggta ttacggttgg tcaacatctt catatagaga tgagtcgttg
    75301 tcggcgttgg ctgtccccaa ccttgcaaat gaccgctgtg aattttcacc ttatcgcctg
    75361 gcaacagtgg cacgacatta ttcatcagca cctgggggaa aatgaaaccc tgtttaatta
    75421 tcgcggcgat aatccgtttt atcaggcgtt aaataaagaa ctacatatta aacgacgggc
    75481 agttattcag gcagtaaacg aaaaacaaaa tatcgccgca gcggtcgcca gtatgatggg
    75541 gttagggatt ggccttacgc catcagccga tgattatctg accggactgg tacttatttt
    75601 atttatttcc gggcatccgg cggaaaaata caaagaggaa ttttatcgcg gtctgcaacg
    75661 cggcagaaat aataccacat tattaagtgc cataacgctg gaagccgcat tacaacaacg
    75721 ctgccgggaa aatattcatc attttattca cgatattatt tatggcgtcc ctgggaattc
    75781 aactcaggca atagaaaaaa ttaaacatat tggctccagt tccggctgcg acatgctgta
    75841 tggcatggcc gatggttgtg cgctgagcca aacctacgga gggaattatg tcagttaaaa
    75901 tagtcattaa accgaatacc tattttgatt ctgtctcgct gatgtctatc tccacgcgtg
    75961 caaataaact cgacggcgtc gagcaggcat ttgtggcgat ggcgaccgaa atgaacaaag
    76021 gtgtgctgaa aaatttagga ctgctgacgc cggagctgga acaggcgaaa aacggcgacc
    76081 tgatgattgt catcaatggt aaatcgggtg cggacaatga gcagttgctg gcggagattg
    76141 aagaactgtt caatagcaaa gcgcaaagcg gctcgcacga ggcgcgttac gccactattg
    76201 ccagcgccaa aaagcatatc ccggaaagta acctggcggt gatttcggtc aacggtctgt
    76261 ttgccgctcg cgaagcgcgt caggcgctgc aaaacgatct caacgtgatg ctgttttccg
    76321 ataacgtctc agttgaagat gaactggcgc tcaagcaact ggcccacgaa aaagggctgc
    76381 tgatgatggg gccagactgc ggtacggcga ttattaacgg cgcggcgctc tgttttggca
    76441 acgccgtgcg tcgtggcaac atcggtattg ttggcgcatc tggcaccggc agccaggaac
    76501 tgagcgtccg cattcatgaa tttggtggcg gcgtttcaca actgattggc accggcgggc
    76561 gcgacctgag cgagaaaatc ggcggcctga tgatgctcga cgccatcggg atgctggaaa
    76621 acgatccgca aactgaaatc attgtgctta tctccaaacc gcccgcgcct gcggtggccc
    76681 gtaaagtgct ggaacgcgcg cgcgcctgcc gcaagccggt ggtggtctgc ttcctcggtc
    76741 gcgttgaaac gccggtggat gagcaggggc tacagtttgc ccgcggcagc aaagaggcgg
    76801 cgctaaaagc ggtgatgctc tccggcgtga aacaggaaaa tctcgacctg catacgctta
    76861 accagccgtt gattgctgat gtgcgtgcgc gtctgcaacc gcagcagaaa tacatacgtg
    76921 gcctgttctg cggcggcacg ctgtgcgacg aaaccatgtt cgcggtgatg gaaaaacatg
    76981 gcgatgtcta cagcaacatt cagcccgatc ctgaattccg cctgcaagat atcaaccgca
    77041 gcatcaaaca caccttcctc gactttggcg atgacgactt caccaacggc aagccgcatc
    77101 caatgattga ccccaccaac cgcatcagtc gcttgatcga agaagcgcgc gatccagaag
    77161 tggcggtgat cgtgatggat tttgtgctcg gatttggatc gcatgaagat ccggtcggct
    77221 ccaccatcga ggcgatcaaa gaagcgaaag cgatcgccgc cgccgaagga cgcgagttga
    77281 tcattctcgc ctatgtgctg ggcaccgatc tcgatacgcc atcattagaa caacaaagcc
    77341 agatgctgct tgatgccgga gtgattctgg cgagcagcag caccaatacc ggattgctgg
    77401 cgcgtgaatt tatctgcaaa ggggaggaag cctgatgagc cagtcactgt ttagccaacc
    77461 gttgaacgtt attaacgtcg gtatcgccat gtttagcgat gacctgaaaa agcagcatgt
    77521 agaagtgact caactcgact ggacgccgcc ggggcagggc aatatgcagg tggtgcaggc
    77581 gctggataac attgccgatt cgccgctggc agacaaaatc gccgccgcta accagcaggc
    77641 gctggagcgc attatccagt cgcatccggt gctgattggt tttgatcagg cgattaacgt
    77701 ggtgccgggc atgacgccga aaaccattct tcacgccggg ccgccgatca cctgggaaaa
    77761 aatgtgcggc gcgatgaaag gcgcggtcac cggggcgttg gtgttcgaag ggctggcgaa
    77821 agatctcgat gaggcgactg aactggcggc ctccggggag atcaccttct cgccgtgcca
    77881 cgaacacgac tgcgtgggat cgatggcggg cgttacctcg gcctcgatgt ttatgcacat
    77941 cgtgaaaaac aaaacctacg gcaacatcgc ttataccaat atgagcgagc agatggcgaa
    78001 gattttgcgt atgggtgcca acgatcagag cgtgatcgac cgcctcaact ggatgcgcga
    78061 tgtgcagggg ccaatgctgc gcgacgcgat gaaaattatc ggtgagatcg atctgcgctt
    78121 aatgctggcg caggcgctgc atatgggcga tgagtgccat aaccgcaata acgccgggac
    78181 gacgctgctg attcaggcac tgacgccggg gatcattcag gcaggctatt ccgtcgagca
    78241 acagcgcgaa gtgttcgagt ttgtcgccag cagcgactac ttctccggcc cgacgtggat
    78301 ggcgatgtgt aaagcggcga tggatgcggc gcacggcatc gaatacagca ccgtggtcac
    78361 caccatggcg cgtaacggcg tcgagttcgg cctgcgggtc agcggcctgc cggggcagtg
    78421 gtttaccggc ccagcgcagc aggtcatcgg cccgatgttt gctggttata agccggaaga
    78481 ttcggggctg gatatcggcg acagcgctat cacagaaacc tacggtattg gcgggtttgc
    78541 catggcgacc gcgcctgcaa tcgtcgcact ggtgggcggc acggtggaag aggctatcga
    78601 tttctcccgt cagatgcgcg aaatcaccct tggcgaaaac cccaacgtca ccattccgct
    78661 gctcggtttt atgggcgtgc cgtcggcaat cgacatcacc cgcgtgggta gcagcggcat
    78721 tctgccggtg atcaacaccg ccatcgccca taaagatgcg ggcgtcggca tgattggcgc
    78781 gggcattgtg catccaccat tcgcctgctt cgagaaagcc attctcggct ggtgcgaacg
    78841 ttacggcgtc tgacttacat gtttatgccg gatgcggtgt gaacgcctta tccggcctac
    78901 ggatggcccg agaattgtag gcctgataag cgtagcgcat caggcagtct ggcgttggtc
    78961 ataacctaat caccctctgt agcggacata acaacatgaa agagcttgtg gtcgttgcca
    79021 ttggtggcaa cagcattatc aaagataacg ccagccagtc gattgagcat caggcggagg
    79081 cggtgaaagc cgtggccgat acggtgctgg aaatgctggc ttccgattac gacattgtgc
    79141 tgacccacgg taacgggccg caggtcgggc tggatttacg ccgtgcggag attgcccacg
    79201 agcgcgaagg gctgccctta acgccgctgg cgaactgtgt ggcagatacg cagggcggca
    79261 tcggctatct gatccaacag gcgctgaata accggctggc gcgtcacggc gagaaaaaag
    79321 ccgtcaccgt ggtaactcag gtggaagtgg ataaaaacga tccaggtttt gcccatccca
    79381 ccaagcccat tggtgcattc ttcagtgaaa gccagcgtga caaactacaa aaggcaaacc
    79441 ctgactggtg ttttgttgaa gatgccggac ggggctatcg ccgcgtggtc gcctcgccgg
    79501 aaccgaaacg tattgtcgaa gcacccgcca ttaaggcgct gatccaacaa ggttttgtcg
    79561 taattggcgc gggcggcggt ggaattccgg tagtgcgtac tgaagcgggg gattaccaaa
    79621 gcgtggacgc ggttattgac aaagatctct ctaccgcgct gctggcccgt gaaattcacg
    79681 ccgacattct ggtgatcacc accggcgtcg aaaaagtctg tatccacttt ggtaaaccgc
    79741 agcagcaggc gctggatcgg gtggatattg ccaccatgac tcgctatatg caggaagggc
    79801 attttccgcc cggcagcatg ttgccaaaaa tcatcgccag cctgacgttc ctggagcagg
    79861 gcggcaaaga agtgattatc accacgccgg aatgcctgcc tgcggcgctg cgcggtgaaa
    79921 cgggcaccca tattattaaa acgtaaggac gtaggatgaa agaaagcaat agccgccgtg
    79981 aatttctgag ccagagcggt aagatggtca ccgccgccgc gctgtttggt acctctgtgc
    80041 cgctcgccca tgcggcggta gctggcaccc taaactgcga agcgaacaac accatgaaaa
    80101 tcactgaccc gcattactat ctcgataacg tgctgctgga aaccggtttt gactacgaaa
    80161 acggtgtggc ggtgcagacc cgcacggcgc gccagaccgt ggagattcag gacggcaaaa
    80221 ttgtcgccct gcgcgagaac aagcagcatc cggacgccac gctgccgcac tatgacgctg
    80281 gcggtaagtt gatgctaccc accactcgcg acatgcatat tcatctcgac aaaaccttct
    80341 acggcgggcc gtggcgctcg ctcaatcgtc cggcaggcac cactatccag gacatgatca
    80401 aactcgagca gaaaatgctg ccggagttac aaccgtatac gcaggagcgg gcggaaaaac
    80461 tgatcgatct attgcagtcg aaaggcacca ccattgcccg cagccactgc aatatcgaac
    80521 cggtttccgg cctgaaaaat ctgcagaatt tgcaggcggt gctggcgcga cgtcaggcgg
    80581 gcttcgagtg tgaaatcgtc gccttcccgc agcacggttt gctgctgtcg aaatcggaac
    80641 ccttaatgcg cgaagcgatg caggcgggtg cgcattacgt cggcgggctg gacccgacca
    80701 gtgttgatgg cgcgatggaa aaatccctcg acaccatgtt tcagattgcg ctggactacg
    80761 acaaaggtgt cgatattcac ctgcacgaaa ccactccgtc gggcgtggca gccatcaatt
    80821 atatggttga aacggtagag aaaacgccgc aactgaaagg taagttgacc atcagccacg
    80881 cctttgcgct ggctacgctc aacgaacaac aggtcgatgc gctggcgaat cggatggcgg
    80941 cgcagcaaat ttctatcgcc tcgacggtgc cgattggcac gctgcatatg ccgctcaaac
    81001 agttgcacga caaaggcgta aaagtgatga ctggcactga cagcgttatc gaccactggt
    81061 cgccttatgg tctgggcgac atgctggaaa aagccaatct ctacgcgcag ctctatattc
    81121 gtcctaacga acagaacctc tcccgttcgc tgtttctagc cactggcgat gtattgccgc
    81181 tgaatgaaaa aggcgagcgt gtatggccaa aagcgcagga tgatgccagc tttgtgctgg
    81241 tggacgcctc ctgttccgcc gaggcggtgg cgcgtatctc gccgagaacc gcaacgttcc
    81301 ataaagggca actggtgtgg gggagtgtgg caggttgatg cgcatttgaa acgcctgcaa
    81361 cggtgaattg taagagattt aagccccagg gagtggggcg ggaatggttt gctaatgaat
    81421 gatcctgaac tttataatct cgcggcataa tttccagggg attatttgag agaaatacac
    81481 aaaagtgagt tttaacaaag gtatttatca ggccataaat gtgacctgct tcggataaaa
    81541 aagattcagt atcgaaaaac caatgatact cttgctgatg gttaccaaca gtaattaaga
    81601 tgtgatttgt ttttccgtaa tcgcaaaaat attgccatga ccataaaaga aaaattctca
    81661 caaaaatatc cccatgcttc cttttgtact tttggcgatt cagcagcgtt ggccgatcac
    81721 ctcgcaacgt tgattgcgac tggtgttaaa acagcttcct gtggttcgct ggcgggatgt
    81781 attgaagaca acgcatttcc gatgattggt gagtataaga ttgtagaaaa tagccgtggt
    81841 gagcctgtat gtgttatcag agtgattggc ctgcatttac tgcgcttttc tgatgtcacc
    81901 gtggagcttg cccgtaagga aggtgaaggt gacctcagtc ttgaatattg gcgtaatgaa
    81961 caccgtcgtt ttttccaggc agaaggaagc tattcaccag aaatggacgt tatatttgaa
    82021 gaatatgccc ttattgacgt tgtgtagata actatttatc tacgcgaaat aaactcacac
    82081 acataaaata tcgctaattt tgtttatttt gctatttttt acgtgatgaa tgtcacactt
    82141 attgttgctg cccgttttgt ccatatttat aaccatattt ttaatagctg cctgaaatag
    82201 ggctgtttat tctgcataaa aatattcgaa aattcttatt ctctccagcg cgctaatttg
    82261 ggcgaacact tcctgactac cctgcaatga ggctgaaatg atgaataaac gttttgttat
    82321 caatatggtg tcttcgctgt tattaggcgc tgcgctaatt tctgcgccgc tccaggcagc
    82381 ggaaaaagtc gtggtgaata tctctaaagt cgatggtatg ccgtggttta atcgcatggg
    82441 cgaaggtgtg gttgaggcgg gtaacgcctt tggtgttaat gcctcacagg tggggccttc
    82501 aagtactgat gctccgcaac aagttaaaat tattgaggat ttaatcgccc gtaaggttaa
    82561 tgcaattact atcgtgccaa acgatgccaa cgtgctggaa ccggtattca aaaaagcgcg
    82621 agatgccgga attgtggttc tgaccaacga atcccccggt cagccaagtg ctaactggga
    82681 tattgaaatc attgataacg aaaaatttgc cgctgaatat gttgagcata tggcgaaacg
    82741 catggggggc aagggcggct atgtgattta cgtgggtagt ttgaccgtgc cgcagcataa
    82801 cttatgggct gacttgctgg tgaaatacca gaaagagcat tacccggata tgcatgaagt
    82861 tacccgccgc atgccggttg ccgagagtgt ggatgactca cgtcgtacca cactggacct
    82921 gatgaaaacc tatcctgact tgaaagcggt tgtctccttt ggttcaaacg gtccgattgg
    82981 cgcgggacgt gcggtgaaag agaaacgggc gaaaaataaa gtcgcagtct atggcatgat
    83041 gatcccttca caggcggcat cactgattaa aagtggtgat atcaccgaag ggattactta
    83101 cgatccggcc tctgcaggtt acgcactcgc agccgtcgcc agcacgttgc tgaagggtga
    83161 agagatcaaa cctggattag aaatgcagaa tctgggtaag gccgacgtag atatggataa
    83221 gcgaattatc cgtttccata aggtattact ggtcaacaaa gacaatatcg actcacttta
    83281 ttaatccatt tcttcggggc gaaagccccg attcttattg ccaggggatg ttatggaaac
    83341 tttcctttcc cttcgtcata tcaacaaaaa gtttcatgcc acgcgagcgc tgcgcgatgt
    83401 ttctctcgac tttatgtcgg gtgaagtgca ctgccttgcc gggcaaaacg gctgtggtaa
    83461 atcgacgttg attaaaatta tgtccggtgt ttatcgcccg gatgagggag cggaaattac
    83521 gcttggtggg aaaaactggt caaagctgac acccgccgct tcggtggcgc aggggattca
    83581 ggtgatttat caggacctct ctttatttcc taacctgagt gtctgggaga atatcgccgt
    83641 gaatcactat caccacggtc tgtttgttaa ccgccgtcgt ctgcgtgagg tggcgcaggc
    83701 ggcaatgacc agtattaacg tcacattgcc actggataca ctcgtttctg agctttcaat
    83761 tgcccgctgt cagctggtgg ctatttgccg tgcactggcg caggacgcac ggttgatcgt
    83821 gatggatgaa ccaaccgctt cgctgaccca tcaggaagtg caagggttat tacaggtggt
    83881 gcatcaattg cgtgaacgcg ggatctgcgt ggtcttcgtc agtcatcgtc tggaagaagt
    83941 gatggaagtt tccgaccgta tttcagtgct gaaagatggt gagctggtcg ggacttttcc
    84001 ggctgcagag atgaccacaa aacaactcgg tttcctgatg accggccagg agtttgaata
    84061 tcaggtgcga gagttgtggc agggaaaatc cagcaccccg gtgctggagg tgcgtaattt
    84121 aagtcgtcat ggggaatatc taaatattaa cttgcgggtg gaagcgggcg aagtggtatc
    84181 gattgttggc ctgcttggtg cggggcgcac ggaattatgt ctgagtctgt ttggtatgac
    84241 ccgactggat gctggcgaga tcctcatcaa cggccagccg gttacactgc atagtaacca
    84301 ggacgctatc cgccacggta ttggttatgt ttcggaagat cgcatgtcgc gcggtttggt
    84361 gatggcgcaa tctattgaag acaacatcat tagcactgtt tttcacaagg taaaagatcg
    84421 cttcggcttt ctgagtgaaa ctaaagttcg tgacctggtg gacaggctaa tcaaggcatt
    84481 gaccatcaaa gtctctgatc ctcatcttcc ggtgaatacg ctttctggtg gtaacgcgca
    84541 gcgagtatcg atcgccaaat ggctggcgat tgggccacga ctgttgattc tcgatagccc
    84601 gacagtcggt gttgatatcg ccaacaaagc aggaatatac ggcattatca gcgatctggc
    84661 ggctcatggc attgcggtat tgatgatctg cgatgaaatt gaagaagcgt ggtatcaaag
    84721 ccaccggatt ttggtaatgc aaaaaggtca gatcacccat agcttcctgc ctgacagcag
    84781 ttctcaggcc aggattgcgg aggtggtaaa tggctgaact gaaaaaacgc catgaattct
    84841 ggctggcatt actgattgtg gtgctgtttg ttggccttgc ctggcgcagc gacgagtttc
    84901 tgacattcgg taatttgtac gatctcgcca ataactatgc catgttgact attctcgcct
    84961 gtggtttgtt tgtggtgttg atttccggtg gaattgatat ttcgtttcca gcaatgacca
    85021 tcattgcgca atacggcatg gtgctgttgc tgcaaaaaat tggtggcaac ttcgctgtcg
    85081 cgtttgcact ggcgggctgc atcggcattt tacttggctt aattaacgcc ttactggtta
    85141 atcgcctacg ggtgccttct atcatcatca ctatctcgac gctgaatatt ttctatggcc
    85201 tgctgttatg gttgagtaaa ggtgtgtggc tgtacgactt tccgccgtgg tttgagcagg
    85261 gggttatgtt gttcaagtac accgatgctg atggctatga ctatggcctt ggtctgccgc
    85321 tgatcgccat gattacggtg gtgctgctaa cagcgtttat catgaatttc accagtgtag
    85381 ggcgcaaaat ttatgccctt ggcgggaacc gcgaatcagc cagtcgcatc ggctttagcg
    85441 tgctgaaact gcaacttttc gtctatggct atatgggatt gatgtctggc gctgcgggtg
    85501 tagtgcagtc gtggacggtg atgactgtcg cccccgattc tcttctgggt tatgagctga
    85561 cagtactggc tgcggtggtg cttggcggca ctagtttgct cggcgggcgc ggcacgttaa
    85621 ccggtacttt gctcggcgtg gtgttgttgg cagtgatgca aaacgggcta aatttattgg
    85681 gagtctcgtc ttactggcaa acattgatca ccggcatcat catcgttgcc agcattagtg
    85741 ccacggcgtg gagtcagcat cagaaccgga gtctgctatg aaaaaatcgt ggcgtaataa
    85801 cgttgagttt tacttgatcg gcctgctggt gctaactgtt gccgcgttca gcattactat
    85861 gccggagatt ttctggtcga taagtaattt ccagtccgtc gcttcgcaaa tgcccgtgct
    85921 gggcattctg gcgttggcta tggcggtgac catgctttgc ggtggcatta acttgtcaat
    85981 tatagccact gctaacgcct gttcactggt gatggcgtgg gtggcgactc agtatccacc
    86041 gggtattgct acggttgtcg ctacgttact ggcgggcgct ggcgcggcgg tgataatcgg
    86101 tttatgtaac ggcgtattga ttgctggtat tcgagtgtcg ccaattcttg caacgcttgg
    86161 gatgatgacg ttgctcaaag ggataaatat tctggtgaca ggtggtagcg ccattgccaa
    86221 ttatccctca tgggtgctgt ggttgaatca tgcgcaatgg ttcggtattc cgctaccgat
    86281 gtggttgttt gccgcagtgg cactggggct atggatattg ctggagaaaa cgccgctcgg
    86341 taaagccatt tacctgattg ggtctaacga acgagcaaca ctttactccg gcattaacac
    86401 tcgtcgggtg ctgatttggg tttatgtcat ttccgccttg ctttgtgcgg tagcggcatt
    86461 tttgatgatg tcgaaactca actcggctaa ggcctcttat ggcgaatcgt acttgctggt
    86521 gtcaatcctc gcggcagtac tcggtggggt taatccggac ggcggcagcg ggcggataat
    86581 tggtatggtg ctggcgctgt ttcttctgca aattattgaa agcggcttca atattctcgg
    86641 tataagcccc tacctgacga tggccttatg gggaacgttg ctgctctgtt tcattcaggt
    86701 tcgcggcatg ttggggctgg atcgggtggt ttaaatattg gctatattca atggacgcgt
    86761 tttgccgcga tgacatatca ggcgtcgcca aatacacata gctaatcagg agtaaacaca
    86821 atgaagatca aagctgttgg tgcatattcc gctaaacaac cacttgaacc gatggatatc
    86881 acacggcgtg aaccgggacc gaatgatgtc aaaatcgaaa tcgcttactg tggcgtctgc
    86941 cattccgatc tccaccaggt ccgttccgag tgggcgggga cggtttaccc ctgcgtaccg
    87001 ggtcatgaaa ttgtggggcg tgtggtagcc gttggtgatc aggtagaaaa acatgcgccg
    87061 ggcgatctgg tcggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa
    87121 gacgggctgg aaaactactg tgatcacatg accggcacct ataactcgcc gacgccggac
    87181 gaaccgggcc atactctggg cggctactca caacagatcg tcgttcatga gcgatatgtt
    87241 ctgcgtattc gtcacccgca agagcagctg gcggcggtgg ctcctttgtt gtgtgcaggg
    87301 atcaccacgt attcgccgct acgtcactgg caggccgggc cgggtaaaaa agtgggcgtg
    87361 gtcggcatcg gcggtctggg acatatgtgg atcaagctgg cccacgcgat gggggcgcat
    87421 gtggtggcat ttaccacttc tgaggcaaaa cgcgaagcgg caaaagccct gggggccgat
    87481 gaagttgtta actcacgcaa tgccgatgag atggcggctc atctgaagag tttcgatttc
    87541 attttgaata cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgt
    87601 gatggcacca tgacgctggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc
    87661 aacctgatca tgaaacgccg tgcgatagcc ggttctatga ttggcggcat tccagaaact
    87721 caggagatgc tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg
    87781 gccgatcaaa ttaatgaagc ctatgagcga atgctgcgtg gtgatgtgaa atatcgtttt
    87841 gttatcgata atcgcacact aacagactga aaaaattaat aaataccctg tggtttaaca
    87901 tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat atcaaagtag
    87961 cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt acacaatact
    88021 tctcacgtta gttatgagcg atttctgata gtgcctggtt taatgagagc tttattatct
    88081 gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct ccgcacaata
    88141 atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat cagttaccag
    88201 aggatatcaa aagctccctg gtttcctgtg tcgatatatt caaggttttg attaaacaat
    88261 attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa ctcattcatc
    88321 tcatggctgc cgtacgagac tgtgagtggt tagacgataa cgcactcacc ataaatgtgc
    88381 agtttaatga tttccctgga ttttatgact ggatggatta ccctgatcac ccggttaagt
    88441 ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac caagatgatg
    88501 cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt aagaaattag
    88561 tgcagtttat cgattcagtg cgaacagatt gcaaatgtat tcttcttgaa caccatatgc
    88621 ctttgctgag aattttccct aaagggaaag agtgtatgcg tgtggaaaaa tggcttcgcg
    88681 aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg catggattat
    88741 tattacactt aaaaaatatc caccctgttt ttcctgaatc actggtgatg ttattgctct
    88801 ctgttctgga cgtaaaaaca tacagagatg acgcacgatt aaatgaatgg atatcgaata
    88861 gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa atccgctaca
    88921 ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga gtgaatcgca
    88981 aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac cgcctttttc
    89041 gccgatattt tgttgtgatc ggcgacgctt cgcttaaaaa agcaccagca gtggtttcgc
    89101 agctatgcgg tgtataaaaa atgatctcat gcagatgttt tgtgaatgtg tgggttgaca
    89161 ttcatatgaa aaaaatcata attccatcat gtttgtgtgg ggtcttttct gtaccttacg
    89221 catcgcactc aagcctgaca gaaaatatgc tgtaaggctc atatcaaaaa ccgccattag
    89281 ctcatcagga agagcagacg acaaccagga ttgttgtatg gtacggggtt cgaggcctcg
    89341 atggcggtcc aacttttcaa aactcttctg aatcagatcc ccttattatc atccctgcaa
    89401 agtggaacat tgccattgtt tggctattca ggacggggca gaccaatgaa aaaagcccac
    89461 acaggggaga gtgggctgaa atgggaagct aaagactcaa gtaaacttat cggaaataag
    89521 gaccacgcat tacgggggct atcatcgccg atggccggga ttcggcaaat caggttgatt
    89581 acgtagagag taaattatct gctcaccgct gcgtcacccc ttcgtaaatc aggcgtagcg
    89641 cgaatacacc aataattgca ccaataaccc gactggcaac gcgttgcata cgcccataag
    89701 cacgacgcac agcgggcaaa gaaaacgcct gactaagaaa aactcgccag ataattgatg
    89761 cgagcacaat ccccgcccag gccattaaac gtgcccatgt tggtgtttcg gcatttaatg
    89821 ttactgagaa aatactaata aaaaataaaa cggtttgcgg gttagagaga tcggtaatta
    89881 atccgcggcg aaaaaagaca taccaggggg cgctaatcgg ttgttgtagt gtgctcattt
    89941 gcggtgttga ctggcggcgc atgctgcacc acgcaaacca taagagataa gcgccgccga
    90001 cgattctgat aagcgaaaaa atctcctcac actgcgtaat tagtgttgca agaccaaaca
    90061 aacccaaccc ggaataaaat gcatcgccca gcgccacccc cagcccggtc agcacacctg
    90121 cgcgtcgacc ggaagccagg ctggtttgta ctaccacaaa gagattggct cccggattaa
    90181 aaaaagtaat cacgaacagt cctacggtca ggtaaacggc gtgcaaagga tccatagtga
    90241 tttcatccat aaataagtga agtaacttca tcatatttct accaaaaata atcgggtgcg
    90301 aaagagatca caaagtgtct tatttccggt tactggcttt tatgccctga ctgaactaat
    90361 tattaatcaa cccaataatg tgggtgggtg atagtgtgat aacaactctg gagccgtaat
    90421 atgaaaataa tctctaaaat gttagtcggt gcgttagcgt ttgccgttac caatgtctat
    90481 gccgctgaat tgatgaccaa agcggaattt gaaaaagttg aatcgcagta tgaaaaaata
    90541 ggtgatattt caaccagtaa tgaaatgtcg accgcagatg cgaaagagga tttgatcaaa
    90601 aaagcggatg aaaaaggggc tgatgtgttg gtactgacct ctggtcaaac tgacaataag
    90661 atccacggca cggcaaatat ttataagaag aagtaattct gaaccctatg taaacatccc
    90721 cgatgcgtaa atttatcggt gatcatcatc tattgaaatt tatgccggat aaagcgttcg
    90781 cgctgcattc ggcagttcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta
    90841 aataattcgc cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca
    90901 gtaagcgtgg agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagctgca
    90961 aaaattgcgg cgttaaatcc ggcgtcggtt ccacacttaa aaatagcgcc agtcgctcca
    91021 tcatattgcg cagttcacga atattgcccg gccagtcgta gtgcaccagc acggtttcgc
    91081 ttgcctgtaa tccctggcgt aatgcggcag aaaacggggc ggagagcgcc gccagagaca
    91141 ctttcaaaaa gctttccgcc agtggcagaa tatccgccac ccgctcgcgc agtggtggca
    91201 attgcagacg caaaatactc agccgataaa acaggtcacg gcgaaactgc ccttgccgca
    91261 tatcttcttc cagattgcag tgagtggcgc taatgacccg cacatccacc ggaacaggct
    91321 gatgcccgcc gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca
    91381 acggcagcgg catttcgcca atctcatcga gaaacagcgt acctccgtgg gcgatttcaa
    91441 acagcccggc gcgaccgccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca
    91501 gttctgcttc cagcagcgat tcggcaatcg ccccgcagtt gactgcaaca aacggatgcg
    91561 actttttgcc ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg
    91621 ccagctcttt gcccgtcccc gtttccccct caatcaacac cgctgcactg gagcgggcat
    91681 acagcaaaat agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca
    91741 gcacgtaacg agttctcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca
    91801 tgcgcgtcat atccagcgca tcgctgaacg cctggcgcac ggtagcggcg gaatagataa
    91861 aaattccggt cattccggct tcttctgcca gatcggtaat cagccccgcg ccgaccaccg
    91921 cttcggtgcc gttagctttt agctcgttaa tctgcccgcg tgcgtcttct tcggtaatgt
    91981 agctacgttg atcgaggcgc aaattaaagg ttttttgaaa cgccaccagc gctggaatgg
    92041 tttcctgata ggtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct
    92101 gtaacacatc gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca
    92161 ggtacgcgcc gttagagcca gccgcgatga tggcgtcaca gcgttcgttt gccagtttct
    92221 tgcggatgta ggtcactgct ttttcaaagc caagctgaat aggggtaatg ttcgccaggt
    92281 gatcaaactc gaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc
    92341 agataaccgg tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta
    92401 agaaaccgaa tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac
    92461 attgcctgaa acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa
    92521 caaattgaaa ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc
    92581 tttatcaacg caaataacaa gttgataaca aaggatgggc tatgtctcta cactctccag
    92641 gtaaagcgtt tcgcgctgca ctgactaaag aaaatccatt gcagattgtt ggcaccatca
    92701 acgctaatca tgcgctgttg gcgcagcgtg ccggatatca ggcaatttat ctttctggcg
    92761 gtggcgtggc ggcaggttcg ctggggctgc ccgatctcgg tatttctacc cttgatgatg
    92821 tgctgaccga cattcgccgt atcaccgacg tttgttcgct gccgctgctg gtggatgcgg
    92881 atatcggttt tggttcttcg gcctttaacg tggcgcgcac cgtgaaatcg atgattaaag
    92941 ccggtgcggc aggattgcat attgaagatc aggttggtgc gaaacgctgc ggtcatcgtc
    93001 cgaataaagc gatcgtctcg aaagaagaga tggtggatcg gatccgcgcg gcggtggatg
    93061 cgaaaaccga tcctgatttt gtgatcatgg cgcgcaccga tgctctggcg gtagaggggc
    93121 tggatgcggc gatcgagcgt gcgcaggcct atgttgaagc gggtgccgag atgttgttcc
    93181 cggaggcgat taccgaactc gccatgtacc gccagtttgc cgatgcggtg caggtgccaa
    93241 tcctcgccaa cattaccgaa tttggcgcaa caccgctgtt taccaccgac gaattacgca
    93301 gcgcccatgt cgcaatggcg ctgtacccgc tttcagcgtt ccgcgccatg aaccgcgccg
    93361 ctgaacatgt ctacaacgtc ctgcgtcagg aaggcacgca gaaaagcgtc atcgatacca
    93421 tgcaaacccg caacgagctg tacgaaagca tcaactacta ccagtacgaa gagaagctcg
    93481 acgacctgtt tgcccgtagc caggtgaaat aaaacacaac gaaaaatatc tcgaccctac
    93541 aaatgacgag gacaatatga gcgacacaac gatcctgcaa aacagtaccc atgtcattaa
    93601 accgaaaaaa tcggtagcac tttctggcgt tccggcgggc aatacggcgc tctgcaccgt
    93661 gggtaaaagt ggcaatgacc tgcattaccg tggctacgat attcttgatc tggcggaaca
    93721 ttgcgaattt gaagaagtgg cgcacctgct gatccacggc aaactgccga cccgtgacga
    93781 actcgccgct tacaaaacga aactgaaagc cctgcgtggt ttaccagcta atgtgcgtac
    93841 cgtgctggaa gccttaccgg cggcgtcgca cccgatggat gtcatgcgca ccggcgtttc
    93901 cgcgctcggc tgcacactgc cagaaaaaga ggggcacact gtctctggcg cgcgggatat
    93961 tgccgacaaa ctgctggcat cacttagctc gattctcctt tattggtatc actacagtca
    94021 caacggcgaa cgcatccagc cggaaaccga tgacgactcc atcggcggtc acttcctgca
    94081 tctgctgcac ggcgaaaagc catcgcaaag ctgggaaaag gcgatgcata tctcgctggt
    94141 gctgtacgcc gaacacgagt ttaacgcctc cacctttacc agtcgggtga ttgcgggcac
    94201 cggctctgat atgtattccg cgattattgg cgcgattggc gcactgcgcg ggccaaaaca
    94261 cggcggggcg aatgaagtgt cgctggagat ccagcaacgc tacgaaacgc cggacgaagc
    94321 cgaagcagat atccgcaagc gcgtggaaaa caaagaagtg gtcattggtt ttggtcatcc
    94381 ggtttacacc atcgctgacc cgcgccacca ggtgattaaa cgtgtggcga agcagctctc
    94441 gcaggaaggc ggttcgctga agatgtataa catcgccgat cgcctggaaa cagtgatgtg
    94501 ggagagcaaa aagatgttcc ccaatctcga ctggttctcc gctgtttcct acaacatgat
    94561 gggcgttccc accgagatgt tcacaccact gtttgttatc gcccgcgtca ccggctgggc
    94621 ggcgcacatt atcgaacaac gtcaggacaa caaaattatc cgtccttccg ccaattatgt
    94681 tggaccggaa gaccgccagt ttgtcgcgct ggataagcgc cagtaaacct ctacgaataa
    94741 caataaggaa acgtacccaa tgtcagctca aatcaacaac atccgcccgg aatttgatcg
    94801 tgaaatcgtt gatatcgtcg attacgtgat gaactacgaa atcagctcca aagtagccta
    94861 cgacaccgcc cattactgcc tgctcgacac gctcggctgc ggtcttgaag ctctcgaata
    94921 cccggcctgt aaaaaactgc tggggccaat tgtccccggc actgtcgtac ccaacggcgt
    94981 gcgcgtcccc ggaactcagt tccagctcga ccccgtccag gcggcattta acatcggcgc
    95041 gatgatccgc tggctcgatt tcaacgatac ctggctggcg gcggagtggg gccatccttc
    95101 cgacaacctc ggcggcattc tggcaacggc ggactggctt tcgcgcaacg cggtcgccag
    95161 cggcaaagcg ccgttgacca tgaaacaggt gctgaccgca atgatcaaag cccatgaaat
    95221 tcagggctgc atcgcgctgg aaaactcctt taaccgcgtc ggcctcgacc acgttctgtt
    95281 agtgaaagtg gcttccaccg ccgtggtcgc cgaaatgctc ggcctgaccc gcgaggaaat
    95341 tctcaacgcc gtttcgctgg cgtgggtgga cggtcagtcg ctgcgcacct atcgccatgc
    95401 gccgaacacc ggcacgcgta aatcctgggc ggcgggcgat gccacttccc gcgcggtacg
    95461 tctggcactg atggcgaaaa cgggcgaaat gggttacccg tcagccctga ctgcgccggt
    95521 gtggggcttc tacgacgtct cctttaaagg tgaatcgttc cgcttccagc gcccgtacgg
    95581 ttcctacgtt atggaaaatg tgctgttcaa aatctccttc ccggcggagt tccactccca
    95641 gacggcagtt gaagcagcga tgacgctcta tgaacagatg caggcagcag gcaaaacggc
    95701 ggcggatatc gaaaaagtga ccattcgcac ccacgaagcc tgtattcgca tcatcgacaa
    95761 aaaaggaccg ctcaataacc cggcagaccg cgatcactgc attcagtaca tggtggcgat
    95821 cccgctgctg ttcgggcgct taacggcggc agattacgag gacaacgtgg cgcaagataa
    95881 acgtatcgac gccctgcgcg agaagatcaa ttgctttgaa gatccggcat ttaccgctga
    95941 ctaccacgac ccggaaaaac gcgccatcgc caatgccatt acccttgagt tcaccgacgg
    96001 cacacgattt gaagaagtgg tggtggagta ccccattggt catgctcgcc gccgtcagga
    96061 tggtattccg aaactggtcg ataaattcaa aatcaatctc gcgcgccagt tcccgactcg
    96121 ccaacagcag cgcattctgg aggtttctct cgacagaact cgcctggaac agatgccggt
    96181 caatgagtat ctcgacctgt acgtcattta agcaaacagc ggtaaggcgt aagttcaaca
    96241 ggagagcatt atgtctttta gcgaatttta tcagcgttcg attaacgaac cggagcagtt
    96301 ctgggccgag caggcccggc gtattgactg gcagacgccc tttacgcaaa cgctcgatca
    96361 cagcaatccg ccgtttgccc gttggttttg tgaaggccga accaacttgt gccacaacgc
    96421 tatcgaccgc tggctggaga aacaaccaga ggcgctggcg ctgattgccg tctcttcgga
    96481 aacagaagaa gagcgcacct ttacctttcg tcagcttcat gacgaagtaa acgcggtggc
    96541 gtcaatgctg cgctcgctgg gcgtgcagcg tggcgatcgg gtgctggtgt atatgccgat
    96601 gattgccgaa gcgcatatta ctctgctggc ctgcgcgcgc attggcgcta ttcattcggt
    96661 ggtgtttggg ggatttgcct cgcacagcgt ggcggcgcga attgatgatg ctaaaccggt
    96721 gctgattgtc tcggctgatg ccggagcgcg cggtggcaaa atcattccct ataaaaaatt
    96781 gctcgacgat gcgataagtc aggcgcagca ccagccacgc catgttttgc tggtggatcg
    96841 cgggctggcg aaaatggcac gcgtcagcgg gcgggatgtc gatttcgcgt cgttgcgcca
    96901 tcaacacatc ggcgcgcggg taccggtggc gtggctggaa tccaacgaaa cctcctgcat
    96961 tctctacacc tccggcacga ccggcaaacc taaaggcgtg cagcgtgacg tcggcggata
    97021 tgcggtggcg ctggcgacct cgatggacac catttttggc ggcaaagcgg gcggtgtgtt
    97081 cttttgtgct tcggatatcg gctgggtggt ggggcattcg tatatcgtct acgcgccgct
    97141 gctggcgggg atggcgacta tcgtttacga aggattaccg acctggccgg actgcggcgt
    97201 gtggtggaaa atcgtcgaga aatatcaggt tagccggatg ttctcagcgc cgaccgccat
    97261 tcgcgtgctg aaaaaattcc ctaccgctga aattcgcaaa cacgatctct cgtcgctgga
    97321 agtgctctat ctggctggag aaccgctgga cgagccgacc gccagttggg tgagcaatac
    97381 gctggatgtg ccggtcatcg acaactactg gcagaccgaa tccggctggc cgattatggc
    97441 gattgctcgc ggtctggacg acaggccgac gcgtctggga agccccggtg tgccgatgta
    97501 tggctataac gtgcagttgc ttaatgaagt caccggcgaa ccgtgtggcg tcaacgagaa
    97561 agggatgctg gtggtggaag ggccgctgcc gccggggtgt attcagacca tctggggcga
    97621 cgacggccgc tttgtgaaga cttactggtc gctgttttcc cgcccggtgt acgccacctt
    97681 tgactggggc atccgtgacg ctgacggtta tcactttatt ctcgggcgca ctgacgatgt
    97741 aattaacgtt gccgggcatc ggctggggac gcgcgagatt gaagagagta tctccagcca
    97801 tccgggcgtt gccgaagtgg cggtggttgg ggtgaaagat gcgctgaaag ggcaggtggc
    97861 ggtggcgttt gtcattccga aagagagcga cagtctggaa gatcgtgatg tggcgcactc
    97921 gcaagagaag gcgattatgg cgctggtgga cagccagatt ggcaactttg gccgcccggc
    97981 gcacgtctgg tttgtctcgc aattgccaaa aacgcgatcc ggaaaaatgc tgcgccgcac
    98041 gatccaggcg atttgcgaag gacgcgatcc tggggatctg acgaccattg atgatcctgc
    98101 gtcgttggat cagatccgcc aggcgatgga agagtaaggt attgtcggat gcgtcgcgtg
    98161 gcgcatccga caccatgcgc cgatgcctga tgcgacgctt accgcgtctt attatgccta
    98221 cccactgttt ttacaccgat aatttttccc ccaccttttt gcactcattc atataaaaaa
    98281 tatatttccc cacgaaaacg attgcttttt atcttcagat gaatagaatg cggcggattt
    98341 tttgggtttc aaacagcaaa aagggggaat ttcgtgtcgc aagataacaa ctttagccag
    98401 gggccagtcc cgcagtcggc gcggaaaggg gtattggcat tgacgttcgt catgctggga
    98461 ttaaccttct tttccgccag tatgtggacc ggcggcactc tcggaaccgg tcttagctat
    98521 catgatttct tcctcgcagt tctcatcggt aatcttctcc tcggtattta cacttcattt
    98581 ctcggttaca ttggcgcaaa aaccggcctg accactcatc ttcttgctcg cttctcgttt
    98641 ggtgttaaag gctcatggct gccttcactg ctactgggcg gaactcaggt tggctggttt
    98701 ggcgtcggtg tggcgatgtt tgccattccg gtgggtaagg caaccgggct ggatattaat
    98761 ttgctgattg ccgtttccgg tttactgatg accgtcaccg tcttttttgg catttcggcg
    98821 ctgacggttc tttcggtgat tgcggttccg gctatcgcct gcctgggcgg ttattccgtg
    98881 tggctggctg ttaacggcat gggcggcctg gacgcattaa aagcggtcgt tcccgcacaa
    98941 ccgttagatt tcaatgtcgc gctggcgctg gttgtggggt catttatcag tgcgggtacg
    99001 ctcaccgctg actttgtccg gtttggtcgc aatgccaaac tggcggtgct ggtggcgatg
    99061 gtggcctttt tcctcggcaa ctcgttgatg tttattttcg gtgcagcggg cgctgcggca
    99121 ctgggcatgg cggatatctc tgatgtgatg attgctcagg gcctgctgct gcctgcgatt
    99181 gtggtgctgg ggctgaatat ctggaccacc aacgataacg cactctatgc gtcgggttta
    99241 ggtttcgcca acattaccgg tatgtcgagc aaaacccttt cggtaatcaa cggtattatc
    99301 ggtacggtct gcgcattatg gctgtataac aattttgtcg gctggttgac cttcctttcg
    99361 gcagctattc ctccggtagg gggcgtgatc atcgccgact atctgatgaa ccgtcgccgc
    99421 tatgagcact ttgcgaccac gcgtatgatg agtgtcaatt gggtggcgat tctggcggtc
    99481 gcgctgggga ttgccgcagg ccactggtta ccgggaattg ttccggtcaa cgcggtatta
    99541 ggtggcgcgc tgagctatct gatccttaac ccgattttga atcgtaaaac gacagcagca
    99601 atgacgcatg tggaggttaa cagtgtcgaa taacgcttta caaacaatta ttaacgcccg
    99661 gttaccaggc aaagaggggc tgtggcagat tcatctgcag gacggaaaaa tcagcgccat
    99721 tgatgcgcaa tccggcgtga tgcccataac tgaaaacagc ctggatgccg aacaaggttt
    99781 agttataccg ccgtttgtgg agccgcatat tcacctggac accacgcaaa ccgccggaca
    99841 accgaactgg aatcagtccg gcacgctgtt tgaaggcatt gaacgctggg ccgagcgcaa
    99901 agcgttatta acccatgacg atgtgaaaca acgcgcctgg caaacgctga aatggcagat
    99961 tgccaacggc attcagcatg tgcgtaccca tgtcgatgtt tcggatgcaa cgctaactgc
   100021 gctgaaagca atgctggaag tgaagcagga agtcgcgccg tggattgatc tgcaaattgt
   100081 cgccttccct caggaaggga ttttgtcgta tcccaacggt gaagcgttgc tggaagaggc
   100141 gttacgctta ggggcagatg tagtgggggc gattccgcat tttgaattta cccgtgaata
   100201 cggcgtggag tcgctgcata aaaccttcgc cctggcgcaa aaatacgacc gtctcatcga
   100261 cgttcactgt gatgagatcg atgacgagca gtcgcgcttt gtcgaaaccg ttgctgccct
   100321 ggcgcaccgt gaaggcatgg gcgcgcgagt caccgccagc cacaccacgg caatgcactc
   100381 ctataacggg gcgtatacct cacgcctgtt ccgcttgctg aaaatgtccg gtattaactt
   100441 tgtcgccaac ccgctggtca atattcatct gcaaggacgt ttcgatacgt atccaaaacg
   100501 tcgcggcatc acgcgcgtta aagagatgct ggagtccggc attaacgtct gctttggtca
   100561 cgatgatgtc ttcgatccgt ggtatccgct gggaacggcg aatatgctgc aagtgctgca
   100621 tatggggctg catgtttgcc agttgatggg ctacgggcag attaatgatg gcctgaattt
   100681 aatcacccac cacagtgcca gaacattgaa tttgcaggat tacggcattg ccgccggaaa
   100741 cagcgcaaac ctgattatcc tgccggctga aaatggattt gatgcgctgc gccgtcaggt
   100801 tccggtacgt tattcggtac gtggcggcaa ggtgattgcc agtacacaac cggcacaaac
   100861 caccgtatat ctggagcagc cagaagccat cgattacaaa cggtaatttc ctctgtgttg
   100921 aagagccact gacctgtgtc agtggctacc gtgattcatt ctcgccaaca tccgcacatt
   100981 cctccaacgc catgtgcaaa aatgccttcg cggcggctgt ctgccagcta tttttccgcc
   101041 gcaacaaaac cgccgttctc tccagtagtg gcggggcaag agaaatcgct ttaagcccgt
   101101 catgttgtgt ggcaatcgct gctggtaaca atgtggaaag ggaagtgcgg cgaatcagct
   101161 ccagaaccgc gctaattgag ttcgcctcaa tgaccacctg tggatgtagc cccgctttct
   101221 cgcagtagtg gtcaatttgc tctctggtgg caaattccgc gctgagcagg accagttttt
   101281 catcatgcaa gcgactcaac gccacctgtt cacaggcagc tagcggatga tgtttcgcca
   101341 ccaccaacgc taaactttct gtcagtaaag gaattgcctc cagctccggc gaatgcacag
   101401 gcgcgaaggc aatcccaacg tccagctcgt cgcgacaaag cagatcttcg attttctcct
   101461 gcgacatttc ctgtagctgg agcgtgatgc cggggtagcg cgcatagaaa tccgccatta
   101521 aagggccgat aaagtagctt gtaaaggtgg gagtgactgc gatacgcagc gatcctcgcg
   101581 tcagatcggc aacatcatga atcgcccgtt tacccgcccc cagttcctgt aacgcccggc
   101641 tggcgtactg tcgccagact tctcctgcat cagtgagacg aatcgttcgc ccgctacggt
   101701 caaacagcgg cacgcctaaa ctctcctcta actggcgaat ctgctgggaa agcgcaggct
   101761 gggagacgtg caacgcactg gcggcacggg taaagctgcc atgttcagcc acggcaagaa
   101821 aatagttgat atgtcgagag agcattcgcc aacctataag taaatccaat agaactcatc
   101881 ataaatgaga cttttacctt atgacaatcg gcgagtagtc tgcctctcat tccagagaca
   101941 gacagaggtt aacggtgaaa gagattattg atggattcct taaattccag cgcgaggcat
   102001 ttccgaagcg ggaagccttg tttaaacagc tggcgacaca gcaaagcccg cgcacacttt
   102061 ttatctcctg ctccgacagc cgtctggtcc ctgagctggt gacgcaacgt gagcctggcg
   102121 atctgttcgt tattcgcaac gcgggcaata tcgtcccttc ctacgggccg gaacccggtg
   102181 gcgtttctgc ttcggtggag tatgccgtcg ctgcgcttcg ggtatctgac attgtgattt
   102241 gtggtcattc caactgtggc gcgatgaccg ccattgccag ctgtcagtgc atggaccata
   102301 tgcctgccgt ctcccactgg ctgcgttatg ccgattcagc ccgcgtcgtt aatgaggcgc
   102361 gcccgcattc cgatttaccg tcaaaagctg cggcgatggt acgtgaaaac gtcattgctc
   102421 agttggctaa tttgcaaact catccatcgg tgcgcctggc gctcgaagag gggcggatcg
   102481 ccctgcacgg ctgggtctac gacattgaaa gcggcagcat cgcagctttt gacggcgcaa
   102541 cccgccagtt tgtgccactg gccgctaatc ctcgcgtttg tgccataccg ctacgccaac
   102601 cgaccgcagc gtaaccttat ttttaaacca tcaggagatc caccatgatt cagtcacaaa
   102661 ttaaccgcaa tattcgtctt gatcttgccg atgccatttt gctcagcaaa gctaaaaaag
   102721 atctctcatt tgccgagatt gccgacggta ccggtctggc agaagccttc gtaaccgcgg
   102781 ctttgctggg gcagcaagcg cttcctgccg acgccgcccg cctggtcggg gcgaagctgg
   102841 atctcgacga agacgccatt ctactgttgc agatgattcc actgcgtggc tgcattgatg
   102901 accgtattcc aaccgaccca acgatgtatc gtttctatga aatgttgcag gtgtacggca
   102961 caaccctgaa agcgttggtt catgagaagt ttggcgatgg cattatcagc gcgattaact
   103021 tcaaactcga cgttaagaaa gtggcggacc cggaaggtgg cgaacgtgcg gtcattacct
   103081 tagatggtaa atatctgccg accaagccgt tctgacagcc atgcgcaatc atcaaaagac
   103141 gttcacgatg ctgctggttc tggtgctgat tggtcttaat atgcgaccac tgctcacctc
   103201 cgtcggtcca ctgctaccgc aattgcgcca ggcgagcgga atgagcttta gcgtggctgc
   103261 cctgttgacc gctctgccgg tggtcaccat gggcgggctg gcgctggccg gaagctggct
   103321 tcatcagcat gtcagcgaac gtcgcagcgt cgccatcagt ctgttgctga ttgccgtcgg
   103381 tgcattgatg cgtgagcttt acccccaaag tgtgctgctg cttagcagcg cactgcttgg
   103441 tggggtgggg atcggtatca ttcaggcggt gatgccttca gtgattaaac ggcggtttca
   103501 gcaacgcacg ccacaggtga tggggctgtg gtctgcggct ctgatgggcg gcggtggact
   103561 tggtgccgcc ataacgccct ggttagttca acatagcgaa tcctggtatc aaacactcgc
   103621 ctggtgggcg ctgcctgccg ttgttgcgct ctttgcctgg tggtggcaaa gcgcccgcga
   103681 ggtcgcctct tcccacaaga caacaaccac tccggttcgc gtggtattca ctccccgcgc
   103741 gtggacgctg ggtgtttact tcggtctgat taacggcggt tacgccagcc tgattgcctg
   103801 gttacccgct ttctatattg agattggtgc cagcgcgcag tacagcggtt ccttactggc
   103861 attgatgacg cttgggcaag ccgcaggagc tttgctgatg cctgctatgg ctcgccatca
   103921 ggatcggcgc aaactgttaa tgctggcgct ggtgttacaa ctggtggggt tctgcggctt
   103981 tatctggttg ccgctgcaat tgccggtctt gtgggcgatg gtgtgtgggt taggtctggg
   104041 cggcgcgttt ccgctctgtt tgctgctggc gctcgatcac tctgtgcaac cggctattgc
   104101 tggcaagctg gtggcgttta tgcagggaat cggttttatc atcgccgggc ttgccccgtg
   104161 gttttctggc gtgctgcgta gtatcagcgg caattacctg atggactggg catttcatgc
   104221 gctgtgcgtc gttgggctga tgattataac cctgcgtttt gcaccagcac gttttccgca
   104281 gctgtgggtc aaagaggcat gatgcgacgc ttgttcctgc gctttgttca tgccggatac
   104341 ggctaatgta gatcgctgaa cttgtaggcc tgataagcgc agcgtatcag gcaattttta
   104401 taatttaaac tgacgattca actttataat ctttgaaata atattgctta tcccggtcgt
   104461 ttatttcgcg aataacccga caaggaacgc cagccgccac gacgtttggt ggaatgtctt
   104521 ttatgacgac actacccgcg ccaataacag aattatcccc gatggtgacg cctggattaa
   104581 taaccacatg acttccgatc cagacgttat tgccaatcgt tatcggaaaa gagtacatct
   104641 caccgttttt tctcaattca tggtgtacag ggtgtcccgt aacggaaaga gtaacattgg
   104701 gtgcaatcaa tacgttatca ccgatcgtta ccgtgtagtc gtcgacaatg gttaaattga
   104761 aatttgcata aaaattgcgg cctatatgga tgttggaacc gtaagagaaa tagacaggtg
   104821 gttctatcca ggcgttttcc cctaccgtgg caaacatttc tttaatcagg ctttctcttt
   104881 tttcaacttc tgatggatgc gagtgattaa actcatacat taacgttttc ccacgaagtc
   104941 ttttttccgg taagccttcg cacatatcgg taaatagctt gcctgctttt attctttctg
   105001 tcatcgacat gttcattgcg atcattccgt cctgatatgt tggtcggata aggcgctcgc
   105061 gccgcatccg acattgattg cttaagcgac ttcattcacc tgacgacgca gtagagaaag
   105121 cgggccgggg ccgctaagcg tgaacacgga aattaaggtg aagcccagcg ccaccagacc
   105181 cagcaccaga taagcgccct ggaaaccgat gctttcatac atattacccg ccagtacaga
   105241 cataaaaatc atcgccagtt gcttaaagaa gcagaaacag accagataaa tcgtcgctga
   105301 aaaacgcact tcaaactggc tggtaatata tttaaagcag cccaccagca ggaacggtac
   105361 ttcaaacata tgcagcgttt tcagaataac cacttccagc gctgaggtgg cgaacgatga
   105421 gccaataata cgtacagaca taatagtgcc agccagcagc agggcgtttt tcccaccgat
   105481 gcgattaatg atcagtggcg caaagaacat aatcgaggcg ttaagtaatt cgcccattgt
   105541 cgttacgtag ccaaataccc gcgtaccctg ttcaccggta gcaaagaacg aagtaaagaa
   105601 attagcaaac tgttggtcaa aaacatcgta ggtgcaggaa acgccaataa catacagtga
   105661 caaaaaccac agttttggct gtctgaacag ttccagcgcc agcttaaggc taaatgccga
   105721 atggttggca cctaccgcat tggcaaccgt ggcggaagag ggcgcatccg ttttggcgaa
   105781 aaagagtaaa acggcgagga tgagtgcaca gccagaaccc agccagaaaa caaactgatt
   105841 attgatggtg aacatgatgc cgacaatcga ggcacacagc gcccagccaa cacagccaaa
   105901 catccgcgcg cgaccaaatt cgaaattact gcgacggctg actttctcaa taaatgcctc
   105961 tactgctggc gcaccggcgt taaaacaaaa gcctagataa ataccaccaa caatcgatcc
   106021 tactaaaatg ttgtattgta acagtggccc gaagataaaa ataaagaacg gcgcaaacat
   106081 cactaacatg ccggtaataa tccacagcag gtatttgcgc agcccgagtt tgtcagaaag
   106141 cagaccaaac agcggttgga ataatagcga gaacagagaa atagcggcaa aaataatacc
   106201 cgtatcactt ttgctgatat ggttgatgtc atgtagccaa atcgggaaaa acgggaagta
   106261 ggctcccatg ataaaaaagt aaaagaaaaa gaataaaccg aacatccaaa agtttgtgtt
   106321 ttttaaatag tacataatgg atttccttac gcgaaatacg ggcagacatg gcctgcccgg
   106381 ttattattat ttttgacacc agaccaactg gtaatggtag cgaccggcgc tcagctggaa
   106441 ttccgccgat actgacgggc tccaggagtc gtcgccacca atccccatat ggaaaccgtc
   106501 gatattcagc catgtgcctt cttccgcgtg cagcagatgg cgatggctgg tttccatcag
   106561 ttgttgttgg ctgtagcgac tgatattgaa ctggaagtcg ccgcgccact ggtgtgggcc
   106621 ataattcaat tcgcgcgtcc cgcagcgcag accgttttcg ctcgggaaga cgtacggggt
   106681 atacatgtct gacaatggca gatcccagcg gtcaaaacag gcggcagtaa ggcggtcggg
   106741 atagttttct tgcggcccta atccgagcca gtttacccgc tctgctacct gtgccagctg
   106801 gcaggtcagg ccaatccgag ccggatgcgg tgtatcgctc gctacttcaa catcaacggt
   106861 aatcgccatt tgaccactac catcaatccg gtaggttttc cggctgataa ataaggtttt
   106921 cccctgatgc tgccacgcgt gagcggtcgt aatcagcacc gcgtcggcaa gtgtatctgc
   106981 cgagcactgc aacaacgccg cttcggcctg gtaatggccc gccaccttcc agcgttcgac
   107041 ccaggcgttc gggtcaatgc gggtcgcttc acttacgcca atgtcgttat ccagcggcgc
   107101 gcgggtgaac tgatcgcgca gcggcgtcag cagttgtttt ttatcgccaa tccacatctg
   107161 tgaaagaaag cctgactggc ggttaaattg ccaacgctta ttacccagct cgatgcaaaa
   107221 atccatttcg ctggtggtca gatgcgggat ggcgtgggac gcggcgggga gtgtcacgct
   107281 gaggttttcc gccagacgcc actgctgcca ggcgctgatg tgtccggctt ctgaccatgc
   107341 ggtcgcgttc ggttgcacta cgtgtaccgt aagccagagt tgtccggcgc tctccggctg
   107401 cggtagtcca ggcagttcaa tcaactgttt accttgtgga gcgacatcca gaggcacttc
   107461 accgcttgcc agcggcttgc catccagcgc caccatccag tgcaggagct cgttatcgct
   107521 atgacggaac aggtattcgc tggtcacttc gatggtttgc ccggataaac ggaactggaa
   107581 aaactgctgc tggtattttg cttccgtcag cgccggatgc ggcgtgcggt cggcaaagac
   107641 cagaccgttc atgcagaact ggcgatcgtt cggcgtatcg ccaaaatcac cgccgtaagc
   107701 caaccacggg ttgccgtttt catcatattt aatcagcgac tgatccaccc agtcccagac
   107761 gaagccgccc tgtaaacggg gatactgacg aaacgcctgc cagtatttag cgaaaccgcc
   107821 aagactgtta cccattgcgt gggcatattc gcaaaggatc agtgggcgca gttctccagg
   107881 tagcgaaagc cattttttga tggaccattt cggcacagcc gggaagggct ggtcttcatc
   107941 cacgcgcgcg tacatcgggc aaataatatc ggtggccgtg gtgtcggctc cgccgccttc
   108001 atactgcacc gggcgggaag gatcgacaga tttgatccag cgatacagcg cgtcgtgatt
   108061 agcgccgtgg cctgattcat tccccagcga ccagatgatc acactcgggt gattacgatc
   108121 gcgctgcacc attcgcgtta cgcgttcgct catcgccggt agccagcgcg gatcatcggt
   108181 cagacgattc attggcacca tgccgtgggt ttcaatattg gcttcatcca ccacatacag
   108241 gccgtagcgg tcgcacagcg tgtaccacag cggatggttc ggataatgcg aacagcgcac
   108301 ggcgttaaag ttgttctgct tcatcagcag gatatcctgc accatcgtct gctcatccat
   108361 gacctgacca tgcagaggat gatgctcgtg acggttaacg cctcgaatca gcaacggctt
   108421 gccgttcagc agcagcagac cattttcaat ccgcacctcg cggaaaccga catcgcaggc
   108481 ttctgcttca atcagcgtgc cgtcggcggt gtgcagttca accaccgcac gatagagatt
   108541 cgggatttcg gcgctccaca gcgccgggtt ttcgacgttc agacgtagcg tgacgcgatc
   108601 ggcataacca ccacgctcat cgataatttc accgccgaaa ggcgcggtgc cgctggcgac
   108661 ctgcgtttca ccctgccaca aagaaaccgt cacccgcagc tcatcgcgca gctcgccgta
   108721 catctgaact tctgcctcca gtaccgcgcg gctgaaatca tcattaaagt gagtggcaac
   108781 atggaaatcg ctgatttgtg tagtcggttt atgcagcaac gagacgtcac ggaaaatgcc
   108841 gctcatccgc cacatatcct gatcttccag ataactgccg tcactccagc gcagcaccat
   108901 caccgcgagg cggttttctc cggcgcgtaa aaatgcgctc aggtcaaatt cagacggcaa
   108961 acgactgtcc tgaccgtaac cgacccagcg accgttgcac cacagatgaa acgccgagtt
   109021 aacgccatca aaaataattc gcgtctggcc ttcctgtagc cagctttcat caacattaaa
   109081 tgtgagcgag taacaacccg tcggattctc cgtgggaaca aacggcggat tgaccgtaat
   109141 gggataggtc acgttggtgt agatgggcgc atcgtaaccg tgcatctgcc agtttgaggg
   109201 gacgacgaca gtatcggcct caggaagatc gcactccagc cagctttccg gcaccgcttc
   109261 tggtgccgga aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg
   109321 gcgatcggtg cgggcttctt cgctattacg ccagctggcg aaagggggat gtgccgcaag
   109381 gcgattaagt tgtgtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
   109441 tgaatccgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattt
   109501 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
   109561 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ttgtcgtgcc
   109621 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
   109681 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
   109741 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
   109801 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactact
   109861 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
   109921 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
   109981 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
   110041 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
   110101 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
   110161 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
   110221 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
   110281 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
   110341 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
   110401 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
   110461 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
   110521 ggaatgtaat tcagctccgc catcgccgct tccacttttt ctcgcgtttt cgcagaaacg
   110581 tggctggcct ggttcaccac gcgagaaacg gtctgataag agacaccggc atactctgcg
   110641 acatcgtata gcgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
   110701 tatcatgcca taccacgaaa ggttttgcgc cattcgatgg tgtcaacgta aatgcacgcc
   110761 gcttcgccgt ccggccacca gaatagcctg cgattcaacc ccttcttcga tctgttttgc
   110821 tacccgttgt agcgccggaa gatgcttttc cgctgcctgt tcaatggtca ttgcgctcgc
   110881 catatacacc agattcagac agccaatcac ccgttgttca ctgcgcagcg gtacggcgat
   110941 agaggcaatc ttctcctcct gatcccagcc gcggtagttc tgtccgtaac cctctttgcg
   111001 cgcgcgcgcc agaatggctt gcagctttaa cggttctcgt gccagttgat agtcatcacc
   111061 ggggcgggcg gctaacattt cgattaattc cttgcgttct tgttccgggc aaaaggccag
   111121 ccaggtcagg cccgaggcgg ttttcaggag cggtaaacgt cgcccgacca ttgcccggtg
   111181 aaaggataag cggctgaaac ggtgggtggt ttcgcgtacc accattgcat caacatccag
   111241 ggtggacaca tctgttggcc ataccacttc gcgcaacaga tcgcccagca gcggggccgc
   111301 cagtgcagaa atccactgtt cgtcacgaaa tccttcgctt aattgccgca ctttgatggt
   111361 cagtcgaaaa ctatcatcgg aggggctacg gcggacatat ccctcttcct gcagcgtctc
   111421 cagcagtcgc cgcacagtgg tgcgatgcag gccgctgagt tccgccagca gcccgacgct
   111481 ggcaccgcca tcaagtttat ttaacatatt taataacatt agaccgcggg ttaagccgcg
   111541 cacggttttg tattccgtct gctcattgtt ctgcatatta attgacattt ctatagttaa
   111601 aacaacgttg tgcacctggt gcacattcgg gcatgttttg attgtagccg aaaataccct
   111661 tcctatactg agcgcacaat aaaaaatcat ttacatgttt ttaacaaaat aagttgcgct
   111721 gtactgtgcg cgcaacggca ttttgtccga gtcgtgaggt actgaaatgg caatacaaca
   111781 ccctgacatc cagcctgctg ttaaccatag cgttcaggtg gcgatcgctg gtgccggtcc
   111841 ggttgggctg atgatggcga actatctcgg tcagatgggc attgacgtgc tggtggtgga
   111901 gaaactcgat aagttgatcg actacccgcg tgcgattggt attgatgacg aggcgctgcg
   111961 caccatgcag tcggtcggcc tggtcgagaa tgttctgccg cacactacac cgtggcacgc
   112021 gatgcgtttt ctcaccccaa aaggccgctg ttttgctgat attcagccaa tgaccgatga
   112081 atttggctgg ccgcgccgta acgcctttat tcagccacag gtcgatgcgg tgatgctgga
   112141 agggttgtcg cgttttccga atgtgcgctg cttgtttgcc cgcgagctgg aggccttcag
   112201 ccagcaaaat gacgaagtga ccttgcacct gaaaacggca gaagggcagc gggaaatagt
   112261 caaagcccag tggctggtgg cctgtgatgg tggggcaagt tttgttcgtc gcaccctgaa
   112321 tgtgccgttt gaaggtaaaa ctgcgccaaa tcagtggatt gtggtggata tcgccaacga
   112381 tccgttaagt acgccgcata tctatttatg ttgcgatccg gtgcgcccgt atgtttctgc
   112441 cgcgctgcct catgcggtac gtcgctttga atttatggtg atgccgggag aaaccgaaga
   112501 acagctgcgt gagccgcaaa atatgcgcaa gctgttaagc aaagtgctgc ctaatccgga
   112561 caatgttgaa ttgattcgcc agcgtgtcta cacccacaac gcgcgactgg cgcaacgttt
   112621 ccgtattgat cgcgtactgc tggcgggcga tgccgcgcac atcatgccgg tatggcaggg
   112681 gcagggctat aacagtggta tgcgcgacgc ctttaacctc gcctggaaac tggcgttggt
   112741 tatccagggg aaagcccgcg atgcgctgct cgatacctat caacaagaac gtcgcgatca
   112801 cgccaaagcg atgattgacc tgtccgtgac ggcgggcaac gtgctggctc cgccgaaacg
   112861 ctggcagggt acgttacgtg acggcgtttc ctggctactg aattatctgc cgccagtaaa
   112921 acgctacttc ctcgaaatgc gcttcaagcc gatgccgcaa tattacggcg gtgcgctggt
   112981 acgtgagggc gaagcgaagc actctccggt cggcaagatg tttattcagc cgaaagtcac
   113041 gctggaaaac ggcgacgtga cgctgctcga taacgcgatt ggcgcgaact tcgcggtaat
   113101 tggctgggga tgcaatccac tgtgggggat gagcgacgag caaatccagc agtggcgcgc
   113161 gttgggcaca cgcttcattc aggtggtgcc ggaagtgcaa attcataccg cacaggataa
   113221 ccacgacggc gtgctacgcg tgggcgatac gcaaggtcgt ctgcgtagct ggttcgcgca
   113281 acacaatgct tcgctggtgg tgatgcgccc ggatcgcttt gttgccgcca ccgccattcc
   113341 gcaaaccctg ggcaatacgc tgaataaact ggcgtcggtg atgacgctga cccgccctga
   113401 tgccgacgtt tctgtcgaaa aggtagcctg atatgcacgc ttatcttcac tgtctttccc
   113461 actcgccgtt ggtggggtat gtcgacccgg cgcaagaggt actcgatgag gtcaatggcg
   113521 tgattgccag cgcccgcgag cgtattgcgg ctttctcccc tgaactggtg gtgctgtttg
   113581 cgccagatca ctacaacggc tttttctatg acgtgatgcc accgttctgt ttaggcgttg
   113641 gagcgacggc aattggtgat ttcggcagtg cggcaggaga gctgcccgtg cctgtggagc
   113701 tggcggaggc ctgtgcgcat gctgtcatga agagcgggat cgatcttgcc gtttcttact
   113761 gtatgcaggt ggaccacggg ttcgcccagc cgctggagtt cctgctcggt gggctggata
   113821 aggtgccagt tctgcctgtg ttcatcaacg gtgtcgccac gccgctgccc ggtttccagc
   113881 gtacccgcat gttgggtgaa gccattggac gtttcaccag cactctcaat aaacgcgtgc
   113941 tgttcctggg ttccggtggg ctttcccatc agccgccggt gcccgaactg gcgaaagccg
   114001 atgcccatat gcgcgaccgt ctgttgggga gcgggaaaga tttacccgcc agtgagcgcg
   114061 aattgcgtca gcaacgggtg attagcgccg ctgagaagtt tgttgaggat cagagaactc
   114121 tgcatccgct caacccgatt tgggataacc agttcatgac tttgctggag cagggacgca
   114181 tacaggaact ggatgccgtc agtaacgaag agctttccgc tattgccgga aagtcgacac
   114241 atgaaatcaa aacctgggtc gccgcttttg ccgctatttc tgcgtttggc aactggcgta
   114301 gcgaagggcg ttattaccgc ccaatcccgg agtggattgc cggatttggc tcgttaagcg
   114361 ccagaacaga gaactgaata tgcaggagaa gatgatgagt tatcagccac aaaccgaagc
   114421 cgccaccagc cgttttctga atgtagaaga agcgggtaaa acgctgcgca tccattttaa
   114481 tgactgcgga caaggcgacg aaaccgttgt cctgctgcat ggttccggcc cgggtgctac
   114541 tggctgggcg aacttcagcc gcaatatcga tccgctggta gaggcgggct atcgggtgat
   114601 cctgctggat tgtccgggtt ggggcaaaag cgattcgatc gttaatagtg gttcacgatc
   114661 ggatcttaat gcacgaatcc tgaaaagcgt ggtggatcaa ctggatatcg ccaaaatcca
   114721 cctgctgggc aactcgatgg gcggccatag ttctgtggcg ttcaccctta actggccgga
   114781 gcgcgtcggc aaactggtgc tgatgggcgg cggtacgggc ggcatgagtt tgtttacgcc
   114841 gatgccaacc gaaggtatta agcgactgaa tcagctttat cgtcagccga ctatcgaaaa
   114901 cctgaagctg atgatggata tcttcgtttt cgataccagc gatttgaccg acgccctgtt
   114961 tgaagcgcgc ctgaataata tgctgtcgcg ccgcgatcac ctggaaaact tcgttaagag
   115021 cctggaagct aatccgaaac agttcccgga ttttggccca cgtctggcgg aaatcaaagc
   115081 gcaaaccctg attgtctggg ggcgcaacga ccgctttgtg ccgatggatg cgggtctgcg
   115141 tctgctgtcc ggcattgccg gttctgaact gcatatcttc cgcgactgtg gtcactgggc
   115201 gcagtgggaa catgccgacg ctttcaatca attggtgctg aatttcctcg cacgccctta
   115261 aggaatggtc atgacgaagc atactcttga gcaactggcg gcggatttac gccgcgccgc
   115321 agagcagggc gaagcgattg cgccgctgcg cgatctgatt ggtatcgata acgctgaagc
   115381 ggcctacgcc attcagcaca taaatgtgca atatgacgtt gcgcaggggc gtcgcgtggt
   115441 agggcgtaaa gtgggcctga cgcatccgaa agtgcagcaa caactgggcg ttgatcaacc
   115501 ggatttcggg acattatttg ccgacatgtg ttatggcgat aacgaaatca ttcctttttc
   115561 ccgtgttctg caaccccgca ttgaagcgga gatcgcactg gtgttgaacc gcgatttgcc
   115621 cgcaaccgat atcaccttcg acgaattgta taacgccatt gaatgggtac ttccggcgct
   115681 ggaggtggtg ggtagccgca ttcgcgactg gtcgattcag tttgtcgata ccgtggcaga
   115741 taacgcttcc tgcggggtgt atgtcatcgg cggtccggcg caacgtccgg cggggttaga
   115801 cctgaaaaac tgcgccatga agatgacgcg taataacgaa gaggtttcca gcgggcgcgg
   115861 cagcgaatgc ctgggacatc cgcttaatgc cgccgtctgg ctggcacgca aaatggccag
   115921 cctgggtgaa ccgctgcgcg ccggagatat cattcttacc ggggcattag gtccgatggt
   115981 ggcagtgaat gcgggcgatc gttttgaagc ccatattgaa ggcataggtt cagttgctgc
   116041 gacattttca agcgcagccc caaaaggaag tctgtcatga gtaagcgtaa agtcgccatt
   116101 atcggttctg gcaacattgg caccgatctg atgattaaaa ttttgcgtca cggtcagcat
   116161 ctggagatgg cggtgatggt cggcattgat cctcagtccg acggtctggc gcgtgccaga
   116221 cgtatgggcg tcgccaccac ccatgaaggg gtgatcgggc tgatgaacat gcctgaattt
   116281 gctgatatcg acattgtatt tgatgcgacc agcgcgggcg cgcatgtgaa aaacgatgcc
   116341 gctttacgcg aagcgaaacc ggatattcgc ttaattgacc tgacgcctgc tgccatcggc
   116401 ccttactgcg tgccggtggt taacctcgag gcgaacgtcg atcaactgaa cgtcaacatg
   116461 gtcacctgcg gcggccaggc caccattcca atggtggcgg cagtttcacg cgtggcgcgt
   116521 gttcattacg ccgaaattat cgcttctatc gccagtaaat ctgccggacc tggcacgcgt
   116581 gccaatatcg atgaatttac ggaaaccact tcccgagcca ttgaagtggt gggcggcgcg
   116641 gcaaaaggga aggcgattat tgtgcttaac ccagcagagc caccgttgat gatgcgtgac
   116701 acggtgtatg tattgagcga cgaagcttca caagatgata tcgaagcctc aatcaatgaa
   116761 atggctgagg cggtgcaggc ttacgtaccg ggttatcgcc tgaaacagcg cgtgcagttt
   116821 gaagttatcc cgcaggataa accggttaat ttgccgggcg tgggtcagtt ctccgggctg
   116881 aaaacggcgg tctggctgga agtcgaaggc gcagcgcatt atctgcctgc ctatgcgggc
   116941 aacctcgaca ttatgacttc cagtgcgctg gcgacagcgg aaaaaatggc ccagtcactg
   117001 gcgcgcaagg caggagaagc ggcatgaacg gtaaaaaact ttatatctcg gacgtcacat
   117061 tgcgtgacgg tatgcacgcc attcgtcatc agtattcgct ggaaaacgtt cgccagattg
   117121 ccaaagcact ggacgatgcc cgcgtggatt cgattgaagt ggcccacggc gacggtttgc
   117181 aaggttccag ctttaactat ggtttcggcg cacatagcga ccttgaatgg attgaagcgg
   117241 cggcggatgt ggtgaagcac gccaaaatcg cgacgttgtt gctgccagga atcggcacta
   117301 ttcacgatct gaaaaatgcc tggcaggctg gcgcgcgggt ggttcgtgtg gcaacgcact
   117361 gtaccgaagc tgatgtttcc gcccagcata ttcagtatgc ccgcgagctc ggaatggaca
   117421 ccgttggttt tctgatgatg agccatatga ccacgccgga gaatctcgca aagcaggcaa
   117481 agctgatgga aggctacggt gcgacctgta tttatgtggt ggattctggc ggtgcgatga
   117541 acatgagcga tatccgtgac cgtttccgcg ccctgaaagc agagctgaaa ccagaaacgc
   117601 aaactggcat gcacgctcac cataacctga gtcttggcgt ggcgaactct atcgcggcgg
   117661 tggaagaggg ctgcgaccga atcgacgcca gcctcgcggg aatgggcgcg ggcgcaggta
   117721 acgcaccgct ggaagtgttt attgccgccg cggataaact gggctggcag catgggaccg
   117781 atctctatgc gttaatggat gccgccgacg acctggtgcg tccgttgcag gatcgaccgg
   117841 tacgagtcga tcgcgaaacg ctggcgctgg gatacgctgg tgtttactcg agcttcctgc
   117901 gtcactgtga aacggcggcg gcgcgttatg gcttaagtgc ggtggatatt ctcgttgagc
   117961 tgggcaaacg ccggatggtt ggcggccagg aggatatgat cgttgacgtg gcgctggatc
   118021 tgcgcaacaa caaataatga tgactgccga gaacgtgcat tttgtatgcc catcccagca
   118081 cgactgccgg atgcggcgtg aacgccttat ccggcctaca tttcgcccgt aggcagtcat
   118141 taaatagttc tgattacgcg gctggcaatg tatcagtcgc gattcacctg cactcgcaat
   118201 gaggttcatc ttatgtcgac tcgtacccct tcatcatctt catcccgcct gatgctgacc
   118261 atcgggcttt gttttttggt cgctctgatg gaagggctgg atcttcaggc ggctggcatt
   118321 gcggcgggtg gcatcgccca ggctttcgca ctcgataaaa tgcaaatggg ctggatattt
   118381 agcgccggaa tactcggttt gctacccggc gcgttggttg gcgggatgct ggcagaccgt
   118441 tatggtcgta agcgtatttt gattggctca gttgcgctgt ttggtttgtt ctcactggca
   118501 acggcgattg cctgggattt cccctcactg gtctttgcgc ggctgatgac cggtgtcggg
   118561 ctgggggcgg cgttgccgaa tcttatcgcc ctgacgtctg aagccgcggg tccacgtttt
   118621 cgtgggacgg cagtgagcct gatgtattgc ggtgttccca ttggcgcggc gctggcggcg
   118681 acactgggtt tcgcgggggc aaacttagca tggcaaacgg tgttttgggt aggtggtgtg
   118741 gtgccgttga ttctggtgcc gctattaatg cgctggctgc cggagtcagc ggttttcgct
   118801 ggcgaaaaac aggctgcgcc accactgcgt gccttatttg cgccagaaac ggcaaccgcg
   118861 acgctgctgc tgtggttgtg ttatttcttc actctgctgg tggtttatat gttgatcaac
   118921 tggctaccgc tacttttggt ggagcaagga ttccagccat cgcaggcggc aggggtgatg
   118981 tttgccctgc aaatgggggc ggcaagcggg acgttaatgt tgggcgcatt gatggataag
   119041 ctgcgtccag taaccatgtc gctactgatt tatagcggca tgttagcttc gctgctggcg
   119101 cttggaacgg tgtcgtcatt taacggtatg ttgctggcgg gatttgtcgc ggggttgttt
   119161 gcgacaggtg gacaaagcgt tttgtatgcc ctggcaccgt tgttttacag ttcgcagatc
   119221 cgcgcaacag gtgtgggaac agccgtggcg gtagggcgtc tgggggctat gagcggtccg
   119281 ttactggccg ggaaaatgct ggcattaggc actggcacgg tcggcgtaat ggccgcttct
   119341 gcaccgggta ttcttgttgc tgggttggcg gtgtttattt tgatgagtcg gagatcaaga
   119401 atgcagccgt gtgccgatgc ctgacgcgcc ttattaagca ggggcgaaag ggacttgccc
   119461 ccaatacacc cacggcgttt acaggtatac tcgctaaaaa ttattcagcg ggtttggaaa
   119521 caaagatggc aaaacttacc ttacaagagc agttgctcaa agcaggatta gtcaccagca
   119581 aaaaagcggc gaaggtcgag agaacggcga aaaaatcgcg cgttcaggcg cgtgaagctc
   119641 gggcggcggt agaagaaaat aaaaaggcac agcttgagcg tgataaacag cttagcgaac
   119701 agcaaaaaca agcggcgttg gcgaaagaat ataaagctca ggtgaagcag ctcattgaaa
   119761 tgaaccgaat caccattgcc aatggcgata ttggttttaa cttcactgac ggcaatctga
   119821 ttaagaagat ttttgtcgat aagctcacac aagcgcagtt gattaatggt cgtctggcga
   119881 ttgctcgctt gttggtcgat aacaatagcg aaggtgaata cgccattatt cccgccagcg
   119941 ttgccgataa aatagctcag cgtgatgcca gcagtatagt gttgcacagc gcgctcagtg
   120001 ccgaagagca ggatgaagac gatccgtatg ccgacttcaa agtgcctgat gatttgatgt
   120061 ggtaaacata gataagtcgt gcgcagatgc ctgatgcgac gctatgcgcg tcttatcatg
   120121 cctacggcct gcgggcgaaa tgtaggcgga taaggcgttc acgccgcatc cggcagtcgt
   120181 gcactattat caacgcatat tcagtttatt ggcgtggtag gcaatatgct cgccaataaa
   120241 actggagaca aaataatagc tgtggtcgta accctcgtga taacggatta acgtcttgat
   120301 attcatctcc tggcagatct tttcaagatt tggagtccgc agctgttccg cgtaaaaatc
   120361 atcactcaac ccctgatcaa ccatgatttc cgcaacgcgt tgaccttgtg aaataagact
   120421 caccggatcg taatccaacc aggcatcttt attttcacca agatatgcgg caaaggcttg
   120481 ctgtccccac ggtacttgcg atggggagac aatgggcgaa aacgccgaga cgctgacata
   120541 ttcatctggg ttacgtaacg ccagcaccag cgcgcccagc ccgcccatag aatgaccaga
   120601 gatagacttt ctggtcgttg ccggaaaatg ttgcatcact aaatccggca gctcgttgcg
   120661 gatatagtta tacattttgt aatgttcatt ccacggcgct tgcgttgcgt tcaggtaaaa
   120721 cccggcacct tgcccgagat cgtaacggtc ggcatctgcg acatgactgc ctcgcggact
   120781 ggtgtccggc gcaacaacaa taatgttgtg ctcagccgcg taacgctgca tccccgattt
   120841 agtaatgaaa ttctgctcgt tgcaggtcag gcccgaaagc cagtacagca ccggcaattt
   120901 ttcattcgcg gcttttggtg ggagatagac gccgacattc atttcacatt tcagtgattg
   120961 ggaataatgc cgatacacat tttgccagcc gccaaagctg gcatgttttt caatgagttc
   121021 catcagtcat tatctcacgg ttaatccccc ggacggctgc ccggggagag tattaagaac
   121081 cccgtctgaa gtggacgggg tatacctgcg ggaaatcagt aacgaattac ggttcgaatg
   121141 gatttgcctt catgcatcag gtcgaaggcg tcattaattt catcaaggct catggtatgc
   121201 gtgacaaacg gttccagatc gatatcacct ttcatcgcat cttcaaccat acccggtaac
   121261 tgggaacgac ctttcacgcc gccaaacgcg gaacctttcc atacgcgacc agtgaccaac
   121321 tggaatggac gggtggagat ttcctgacca gaacctgcca ccccgatgat caccgactga
   121381 ccccagccgc ggtgcgcact ttccagcgcc gcacgcatca cgttgacgtt accgatgcat
   121441 tcaaaggtat ggtcgatacc ccatttgttg atatccagca ggacatcttt aatcggtttg
   121501 tcgtagtcat tcgggttaat gcagtcggta gcaccgaagc gacgcgccag atcgaatttc
   121561 ttcgggttgg tatcgatagc gataatccgt cccgctttcg cctgacgtgc gccctgaacc
   121621 actgccagac caatcgcgcc aagaccaaac acggcaacag aatcacctgg ctggacttta
   121681 gctgtgttgt gcaccgcgcc aataccggtg gtcacgccac agcccagcag acagacgtgt
   121741 tcatggtttg cttctggatt aattttggcc agagacactt ccgcgaccac ggtgtattca
   121801 ctgaatgtag agcaccccat gtagtgataa agcggctgcc cgttgtaaga aaaacgggtg
   121861 gtgccgtctg gcatcaagcc tttaccctgg gtttcgcgaa ccgcaacaca gaggttagtt
   121921 ttgccagaac gacagaactc acactcgccg cactccgcgg tgtaaagcgg gatcacatgg
   121981 tcgccaggtt tgacgctggt tacgccttca ccgacttcaa ccacaacgcc ggccccttcg
   122041 tgaccgagaa ccaccgggaa tacaccttcc gggtcatccc cggagagggt aaatgcgtcg
   122101 gtatggcaaa cgccggtatg ggtgacttta atcagcactt cacctttttt cggtggtgca
   122161 acgtcaattt caacgatttc cagcggttta ccgggagcaa atgcaacggc agcacgtgat
   122221 ttcatctctc gctcttcctc aatatggtaa tagattcagc tatttaagat aggctcgaac
   122281 cagttcaata gtgtcgtcaa cggattggct gacttcgcgg ctgtagcagt catttcggtc
   122341 aaacgtttcc cggatatggc tttcaagcac ttctgccatc agcccgttag ccgcgccccg
   122401 aacggcagcg atctgttgga gtatggcacg gcattcggca tcaccctcca gcgaccgttc
   122461 cagagcatca atctgccctc gaatacgacg aactcgagta aggacctttt tcttctcttc
   122521 cggagtactg ggcatttcgc acctcatcat ctgcacgcaa tatactatag gggggtatgc
   122581 tatatgtcaa tgcatacccc cctatagtat ataacactgg agaataaaat ttatccggtg
   122641 aatgtggtcg gaaaacaaag aggaaagggg gggctaatcg gcagggaagg ccgccccgga
   122701 tagcgggcgg cagaaggaat cagaatttcc aggtcagatg ggctgcaagt tgcagaccgt
   122761 tataatcatc ggttggggtg tcgtaccaca ctttacctgc cgtcagcccg agattaagtt
   122821 gctcagtcag cggttgaata cgttgcaaac tgacgctctt catgctgccg tagcgtgttt
   122881 ctggcgtcca gtcataggtg taagcgcctg ttccgcggct tagccatagt tgcgtataac
   122941 cagtgccgcg cgggtcattc agacgcacgg aaatcatatt gctataacta ccacctgcat
   123001 cgctggagtc ataatgggta tagcggtagc tggtgatcac cgggccagta tagagtgata
   123061 cgccgccttg ccaggcatcg acttcgacat catcgtaata tttagtgtag cgataaccgg
   123121 tcgtgaaaag cgtttttggc aacagggcca ggttaatatc ctgacgaaaa tcctggcggg
   123181 caaagaccgg cgtattatcc gcaaaggcaa tacccgttcg ggttgtcagc cagttattcc
   123241 atttatacca gaccgcaccc tgaccgcgag tggcattgaa attttcagtt tcataatcac
   123301 gacgcccctg ggaaagatta aaaagcagcg ttgcgttttc aactttcgcc accagttcag
   123361 catacgctaa attacggttg ccatgatcgc cagaataatc ggtaaaatcg tagcccgcgg
   123421 taatggaagt taacccggca taagcgggca atgcgctaaa aatggccgcc gctaataacg
   123481 tccgttttat cattttaatc tcctgtacgg ataagttctt gtcggagtga ataagttccg
   123541 gcaggtaaat tacgttctac aatcacgcgc gggccaagtt gcgtgttcgg ggaaataatt
   123601 cgcccaggca aaattataac ttgtacgcca aggcgtgaac gctgcccgat ataacaacct
   123661 aatttatcgc atccggtagc gataattccc tctggagttc gaacagacac aggctgttca
   123721 tccagacgat gattactggt acgtacttgt gcgcccaaat atgcctggtt tgcaactacc
   123781 gagtcggcaa taaaacattg cggaccaatc gttgcttccg cttcaataac cgcattttta
   123841 atttcggtgg caaaaccaat ttttacacca ttgctgatta ttgtgccagg acgaataaac
   123901 gcataattac ctatcaggca gtttgcgcca atcactaccg gaccctgaat aacggcacca
   123961 tgacaaatac gggtattcgc gccgataaca acttcacctg cagtttcatc aataattacc
   124021 tggtctgcaa tctgaatatt tttctcttta acattaagct tcagatagtt tttcagttcc
   124081 tgtgacagaa ggttgggaat aagcggttga cgcttccacc agtgcggaat agcgggtggg
   124141 tttgtcgcgc tgaggttcgc gtccagtaaa aaaggcaata agtccataaa taatccagat
   124201 ggcataagct aataatacat acacaacgga aagcggcgct aaaggcacca acaaccagca
   124261 acgatgaaac caggcgctaa aagcaccaat aacacaaact acgccgatcc agataagcgg
   124321 aaacattgcc aacaccacac catgcggccc ggtggtgatg aattcattaa accaggtagt
   124381 gagataaatc ccaacgccat aaatcacaac caacagcata ggaaatatac tgaagatacc
   124441 gaagcggcta aataaaagtc ttttatgcag gcgcatacag accgcgtatc ccacaatcca
   124501 acgccgccag cgacgccact cctcacgcgg actgttgcat tcctgtgggt atacgatgca
   124561 gcgattcgcc tgccgaatac ggtagccgtt tgccactaat gtccaggtga gatcaaggtc
   124621 ttcgacttta gtacgatccg agaaaccgaa cttacgcaat acatcagtac ggaacatccc
   124681 gcaggcaccg ctgataataa acggtgcgcc gcccaggagc tgctgtagcg tgcgcttcat
   124741 aacaatcatc ggcaacttta cggtcgcgcg gatgtgcggt aacagccccg cgcctttcaa
   124801 cgcagtagag ggaatgccgc ctacggcatc agcaccgcgc tcaatttctg ccagcatata
   124861 gcccattccg tcttgatcgg gcggaacata ggtgtcggca tcacttaaaa aaacctggtc
   124921 gcaggtggca taattgaggc cattcatcag cgcaccacct ttaccagtat ttttttgcgt
   124981 gacggcaaca aagcggtcgc cccatttgcg tttgacttcc gccatgaccg cttcggtatt
   125041 gtccgtggag ccgtcgttaa cgcaaattac ccggcaaaaa taagggttac gcagtagatt
   125101 atccagtgac tgcgccagac acgggccttc gttatacgca ggtataatgg cgtcaataca
   125161 gcctttcttt tgactgggtt tacgtcttag cgtacttaaa aaccatagca acattgctat
   125221 ggacatacag ataaatatcc aggttttcat gcaatttgct caaagaatca ttttatgaat
   125281 tacaaagcct tcacccagat cgctattgac ctgttgcccg cgaaattgtg caactgcacg
   125341 caggcgatca tgacgcatat aatcacgtcg ttcctggctt ttatgttttt ttaatgcagc
   125401 aagtttgact gtgaaatatt cttctttaac ggattcaaaa acctgaggca taaatgaaag
   125461 ccaggtactt ggggtttcgt agccgagaat ttgtggaata gtgcggcagg caaccattga
   125521 agcttgataa acagcgagat gatcctgatg gcggtcggca tcatgcatgg tatatacccg
   125581 catgatttca acatcagaag gaatttgatt tttaatgatg tcttccaggg cggaaatcat
   125641 atcattgagc tgtaaatgag cgcgggtgtc agcaaaatta agatgaatag tttggtggca
   125701 ccctaatatc tttagggcgt tgcgcgattc ttcatggcga tcgattattc catctgtacc
   125761 agagttgccg gtagtcatca ccactgctgc gatataaatt cctttttgcg caagacgagc
   125821 aagcgatgcg ccacagccta attctatatc gtcaggatgt gcgccaatag caaggatacc
   125881 ctttctttta tttgccgaag aaaggagggc tgaatctaaa accttatcca cttaatgaca
   125941 ctccatttta tttattatac tacaagcaca acgatgcact cagagacgta atctctggcg
   126001 cagccagatg ttaatttata aatgttaaat gttgctaata actgaaaagc aagggctttc
   126061 agacccggag gttcgactgg gtaatgtttt tatgcaattc atatgttaag tgtttgtatg
   126121 tttggtatgt atagttattt tgttttatac attgtattta gatgtgattt agtttgtcaa
   126181 ttaattgcat ttaaaaaata tgttctgtga acaagtattg tttatataca ttatgtgaat
   126241 gtaatatgcg agtgattgag aatgatacag tgatatatac aatgcgaata taatagtttt
   126301 attatatgta ttgatattga tagaaataat gaagtaataa atctcgtaat gtggttgttt
   126361 atgcatcact aaaatgaagt gtagtaattt tcccaattgt tagaacggag taattgcata
   126421 tttaatcttt ccttagccgt ttttttgcta agaataaaat catctgtgcg ataacgacta
   126481 attcttttaa tgaatgtttt tattcctgaa tactgctccc ataacaagac aggggagcag
   126541 acaatcatgg caatttcatc gcgtaacaca cttcttgccg cactggcatt catcgctttt
   126601 caggcacagg cggtgaacgt caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg
   126661 gctcaggccg acaacacctt tgctaaagaa agcggagcaa ccgtggactg gcgtaagttt
   126721 gacagcggag ccagcatcgt gcgggcgctg gcttctggcg acgtgcaaat cggcaacctc
   126781 ggttccagcc cattagcggt tgcagccagc caacaggtgc cgattgaagt cttcttgctg
   126841 gcgtcaaaac tgggtaactc cgaagcgctg gtggtaaaga aaactatcag caaacccgaa
   126901 gatttgattg gcaaacgcat tgccgtaccg tttatctcca ccacccacta cagcctgctg
   126961 gcggcgctga aacactgggg gattaaacca gggcaagtgg agattgtgaa cctgcagccg
   127021 cccgcgatta tcgccgcatg gcagcggggt gatattgatg gtgcttatgt ctgggcaccg
   127081 gcggttaacg ccctggaaaa agacggcaag gtgttgaccg attctgaaca ggtcgggcag
   127141 tggggtgcgc caacgctgga cgtctgggtg gtgcgcaaag attttgccga gaaacatcct
   127201 gaggtcgtga aagcgttcgc taaaagcgcc atcgatgctc agcaaccgta cattgctaac
   127261 ccagacgcgt ggctgaaaca gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg
   127321 ccggaaggtg acgttccggg gctggtgaag gggaatacct atctgacgcc gcagcaacaa
   127381 acggtagaac tgaccggacc ggtgaacaaa gcgatcatcg acaccgcgca gtttttgaaa
   127441 gagcagggca aggtcccggc tgtagcgaat gattacagcc agtacgttac ctcgcgcttc
   127501 gtgcaataaa aggaggcgca gatgctgcaa atctctcatc tttacgccga ttatggcggc
   127561 aaaccggtac tggaagatat caacctgacg ctggaaagcg gcgagctact ggtggtgctg
   127621 gggccgtccg gctgcggtaa aaccaccctg ctgaatctga ttgccggttt tgtgccttat
   127681 cagcatggca gcattcaact ggcgggtaag cgtattgagg gaccgggagc agagcgcggg
   127741 gtggtttttc agaatgaagg gctactaccg tggcgcaatg tacaggacaa cgtggcgttc
   127801 ggcctgcaat tggcaggtat agagaaaatg cagcgactgg aaatcgcgca ccagatgctg
   127861 aaaaaagtgg ggctggaagg cgcagaaaaa cgctatatct ggcagctttc cggtggtcaa
   127921 cgtcagcggg tggggattgc ccgcgcgctg gcggcgaatc cccagctgtt attactcgac
   127981 gaaccgtttg gtgcgctgga cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa
   128041 ctctggcagg agacgggcaa gcaggtgctg ttgattaccc acgatataga agaagcggtg
   128101 tttatggcga ctgaactggt tctgctttca tccggccctg gccgtgtgct ggagcggctg
   128161 ccgctcaact ttgctcgccg ctttgttgcg ggagagtcga gccgcagcat caagtccgat
   128221 ccacaattca tcgccatgcg cgaatatgtt ttaagccgcg tatttgagca acgggaggcg
   128281 ttctcatgag tgtgctcatt aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc
   128341 cgctctcgcg tcaggtgacc ttaagcattg gcacgttagc ggttttactc actgtatggt
   128401 ggacggtggc ggcgctgcaa ctgattagcc cgctgttttt gccgccgccg caacaagtac
   128461 tggcaaaact gctcaccatt gccggaccgc agggatttat ggacgccacg ctgtggcagc
   128521 atctggcagc cagcctgacg cgcattgtgc tggcgctact cgcggcggtg gtgatcggta
   128581 ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg cggcattctg gatccggtaa
   128641 tcgaacttta tcgtccggtg ccgccgctgg cttatttgcc gctgatggtg atctggtttg
   128701 gtattggtga aacctcgaag atcttactga tctatttggc gatttttgca ccggtggcga
   128761 tgtcggcgct ggcgggggtg aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc
   128821 tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa
   128881 tcctcaccgg attacgcatt ggtctgggtg tgggctggtc tacgctggta gcggcggagc
   128941 tgattgccgc gacgcgcggt ttaggattta tggttcagtc agcgggtgaa tttctcgcga
   129001 ctgacgtggt gctggcggga atcgcggtga ttgcgattat cgcctttctt ttagaactag
   129061 gtttgcgcgc gttacagcgc cgtctgacgc cctggcatgg agaagtacaa tgagtgaacg
   129121 tctgagcatt accccgctgg ggccgtatat cggcgcacaa atttcgggtg ccgacctgac
   129181 gcgcccgtta agcgataatc agtttgaaca gctttaccat gcggtgctgc gccatcaggt
   129241 ggtgtttctg cgcgatcaaa ctattacgcc gcagcagcaa cgcgcgctgg cccagcgttt
   129301 tggcgaactg catattcacc ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt
   129361 gctggatacc cataacgata atccgccgga taacgacaac tggcataccg atgtgacatt
   129421 tattgaaacg ccacccgcag gggcgattct ggcagctaaa gagttacctt cgaccggcgg
   129481 tgatacgctc tggaccagcg gtattgcggc ctatgaggcg ctctctgttc ccttccgcca
   129541 gctgctgagc gggctgcgtg cggagcatga tttccgtaaa tcgttcccgg aatacaaata
   129601 ccgcaaaacc gaggaggaac atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt
   129661 gctacatccg gtggtgcgaa cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga
   129721 aggctttact acgcgaattg tcgatgtcag cgagaaagag agcgaagcct tgttaggctt
   129781 tttattcgcc catatcacta aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga
   129841 tattgcgatt tgggataacc gcgtgactca gcactatgcc aatgccgatt acctgccaca
   129901 gcggcggatt atgcatcggg cgacgatcct tggggataaa ccgttttatc gggcggggtg
   129961 atacgagtgc ggacggttcc ctcgccccct tggggagagg gttagggtga gggggcgttc
   130021 accgtacttt caacaggtta actccccctt tctgagagga aaaaaattaa cgcagaatct
   130081 tcttctcagc caaatccagc gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa
   130141 ttgaacctaa gctttcgagc acgactttct cttcatctat agcacccgcc agcgcggcga
   130201 acttaatcat cgcgtactca ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt
   130261 cacgcagctc acgcacgatg tcgaggtatg ctccagcagg tttaaccatc aggcagtctg
   130321 cgccctgggc ttcatccagc agtgactcac gaatcgcctc gcgacggttc attgggttca
   130381 tctgatagct tttgcggtcg ccttttaatg cgcttccggc agcttcacgg aacgggccat
   130441 aaaaggaaga ggcgaacttg gtcgaatacg acataatcgc cgtatcttta aagcctgcag
   130501 cgtccagcgc ctgacgaatc gcctgtacct ggccgtccat cgcggcggaa ggggcgatga
   130561 agtctgcgcc tgcagcagct gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt
   130621 cgttgtcgac gccatgctcg cacagcacac cgcagtgacc gtgagaagtg tattcacaga
   130681 aacaagtgtc tgacatgacg atcatttctg gcacagtctg cttgcagatg cgcgacatac
   130741 gcgccaccag tccatcttcc cgccaggcgt cgctgccggt ttcatcggta tggtgagaga
   130801 tgccaaaagt cattacggaa cggataccgg cgttggcgat gcgttcaatt tcgcgtgcca
   130861 gatgtttctc tggaatgcgc atcacgcctg gcatggcttc aacggctttg tagtcgtcaa
   130921 tttcttcttc aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa
   130981 acatagcgcg cagcgcagga gatttgcgca ggcgacgagg gcgttggatt aagtctgtca
   131041 tggtctgcct gatgtttgtg gaatcgaggg gcatagtata cctgaagcag ggtagggatg
   131101 ttttacgaaa gttgtcgcga tgttgacaag aagagaatgg aagagaggcc aggacatctt
   131161 tgtaatagaa atagcaataa tttcatattc actgaaaata tttttaatct ttatttatag
   131221 ctcttggtta ttattttttt ggcatttggt tgtgctgcta taaattgaat aattcaaact
   131281 ttgttgtata ttgataagat atttatctgg tttgtgtttt tgatatttat aacgcgtatg
   131341 tatcaaaaat aacattatct gcaacatgtt gaaatatcgt ccttttatca atttgatgcg
   131401 tgtttttctc cataaattat atatgtccac attcggactt aggggaaaga ataattgaac
   131461 cattcgtctg taacgcagca taatcgttag cgcgaaacat aatatgtttt ctatgcattg
   131521 ataattgatg gatcaactta ttacgtccct gaggagggat gacaaatgca ctcctggaaa
   131581 aagaaacttg tagtatcaca attagcattg gcttgcactc tggctatcac ctctcaggct
   131641 aatgcagcaa actatgatac ctggacttat atcgataatc ccgttacagc acttgattgg
   131701 gatcatatgg ataaggcagg cactgtagat ggcaactatg taaactatag tggttttgtc
   131761 tattacaaca acaccaatgg tgatttcgat cagtccttta acggcgatac cgttaacggg
   131821 acaatctcaa cctattattt gaaccatgat tatgcagaca gtactgctaa tcagcttgat
   131881 atcagtaatt cagtgattca cggttcgatt acttctatgc tgcctggcgg ttattatgat
   131941 cgttttgatg cagatggtaa taatctgggt ggatatgatt tttacactga tgcggttgtt
   132001 gatacacact ggcgtgatgg tgatgttttc actttgaaca ttgctaacac tactattgat
   132061 gatgattatg aagctcttta cttcactgat tcttataaag atggtgatgt aaccaagcac
   132121 acaaatgaga catttgatac aagtgaaggc gttgctgtta atcttgatgt agaaagtaac
   132181 atcaatattt ccaataactc ccgcgttgca ggtattgcat tatctcaagg taatacttac
   132241 aacgaaacct acactaccga atctcatact tgggataaca atatctctgt aaaagattcc
   132301 acagtgactt cgggttcaaa ttatatcctg gatagcaata cttatggcaa aactggtcac
   132361 tttggcaatt ctgatgaacc gagtgattat gctggcccgg gtgatgttgc aatgtccttt
   132421 actgcttcag gttccgacta tgcgatgaag aacaatgtat tcctcagcaa ttcaacgctg
   132481 atgggtgatg ttgcctttac cagcacctgg aatagtaatt ttgatccgaa tggtcatgat
   132541 tccaacggtg acggggtgaa agataccaac gggggttgga ctgatgatag cctcaacgtt
   132601 gatgaactaa atctcactct cgataacgga agcaagtggg ttggtcaggc aatttataac
   132661 gttgctgaaa cgtcagcaat gtatgatgtt gctacaaaca gccttactcc tgatgcaaca
   132721 tatgaaaaca atgactggaa acgtgttgtt gatgacaagg tcttccagag cggtgtattt
   132781 aacgtagcgt tgaataacgg ttctgaatgg gatactacag gtcgttccat cgttgatacc
   132841 ttgacagtta ataatggttc tcaggttaat gtttcggaat ctaaattaac ttcagatact
   132901 atcgatttaa ctaacggttc ttcgctgaac attggtgaag atggctacgt tgataccgat
   132961 catctgacta ttaactccta cagtactgtt gcgttgaccg aatctactgg gtggggggct
   133021 gattacaacc tgtacgccaa tactatcacc gtaactaacg gtggtgtatt ggatgtgaac
   133081 gttgatcagt tcgatactga agctttccgt actgacaaac tggaactgac cagcggcaac
   133141 atcgctgacc ataacggtaa cgtagtatct ggtgtgttcg atatccatag cagcgattac
   133201 gttctgaacg ctgatctggt gaacgaccgt acgtgggata cttccaagtc taactacggt
   133261 tacggtattg ttgctatgaa ctctgacggt cacctgacta tcaatggtaa cggcgacgta
   133321 gacaacggta ctgaactgga taacagctct gttgataacg ttgttgctgc aaccggtaac
   133381 tacaaagttc gtatcgacaa cgcaactggc gctggcgcta tcgctgatta caaagataaa
   133441 gaaattatct acgtaaacga cgtcaacacc aacgcgacct tctctgctgc taacaaagct
   133501 gacctgggtg catacaccta tcaggctgaa cagcgcggta acaccgttgt tctgcaacag
   133561 atggagctga ccgactacgc taacatggca ctgagcatcc cgtctgcgaa caccaatatc
   133621 tggaacctgg aacaagacac cgttggtact cgtctgacca actctcgtca tggcctggct
   133681 gataacggcg gcgcatgggt aagctacttc ggtggtaact tcaacggcga caacggcacc
   133741 atcaactatg atcaggatgt taacggcatc atggtcggtg ttgataccaa aattgacggt
   133801 aacaacgcta agtggatcgt cggtgcggct gcaggcttcg ctaaaggtga catgaatgac
   133861 cgttctggtc aggtggatca agacagccag actgcctaca tctactcttc tgctcacttc
   133921 gcgaacaacg tctttgttga tggtagcttg agctactctc acttcaacaa cgacctgtct
   133981 gcaaccatga gcaacggtac ttacgttgac ggtagcacca actctgacgc gtggggcttc
   134041 ggtttgaaag ccggttacga cttcaaactg ggtgatgctg gttacgtgac tccttacggc
   134101 agcgtttctg gtctgttcca gtctggtgat gactaccagc tgagcaacga catgaaagtt
   134161 gacggtcagt cttacgacag catgcgttat gaactgggtg tagatgcagg ttataccttc
   134221 acctacagcg aagatcaggc tctgactccg tacttcaaac tggcttacgt ctacgacgac
   134281 tctaacaacg ataacgatct gaacggtgat tccatcgata acggtactga agggtctgcg
   134341 gtacgtgttg gtctgggtac tcagttcagc ttcaccaaga acttcagcgc ctataccgat
   134401 gctaactacc tcggtggtgg tgacgtagat caagactggt ccgcgaacgt gggtgttaaa
   134461 tatacctggt aatattcttc actccgaaga aattctggta atttaatcta aataatgccc
   134521 gtcaaggatt tgacgggcat tactgcaaag gacgcgcaaa tgttatctgt agttaaacct
   134581 cttcaggaat ttggtaagct cgataaatgt ttgtccagat acggtacgcg cttcgagttt
   134641 aataatgaaa agcaagttat attttccagt gatgtcaata gcgaagatac tttcgttatt
   134701 ttagagggcg ttatctctct gcgtagagaa gaaaacgtac ttatcggtat tacccaggct
   134761 ccttatatta tggggctggc tgatggttta atgaaaaacg atataccata caaattaata
   134821 tcagaaggaa attgtacggg atatcatcta ccagccaaac aaaccattac gcttattgaa
   134881 caaaatcaac tctggcgaga cgctttttac tggttagcct ggcaaaatag aattctggaa
   134941 ttacgcgacg tgcagctcat tgggcataat tcctacgaac aaatccgcgc aacattatta
   135001 tcaatgattg actggaatga agaattgcga tcacgtattg gtgtgatgaa ttatatccat
   135061 caacgtacac gcatatcgcg ttctgtcgtc gcagaagttc tcgctgcttt gcgtaaaggc
   135121 ggctatatcg aaatgaataa aggcaaactg gtcgctatca accgtttgcc ttcagagtat
   135181 taatcaggat gcggggataa ccaacggttt attcccgctt agctcggtca ccagatcatt
   135241 gatgccatcg ctcatatttt taaagcgcgt caacggcgaa cgagtgacca ccacaaacgc
   135301 gccaatattc ttctgcggga tcatcgccat ataggtaatg aaaccgccac cacctcctgt
   135361 cttctgaata atccccggac gaccctcttt cggggccata tatacccagc ctaaaccaag
   135421 cgcatccgct ttgccgggga catccatgcc aatcactttc gtaaactgcg cgcgctgata
   135481 aatcaacgtc tgcatgcgat ctgcctggtt actgcgctga taaaaatcag atgacaaata
   135541 ctgctgcatc cagcgcatca tatcgccagg cgtggaatag acgccgccgc tgccaattgc
   135601 cgccagcgtg ttattgcacg gactggcacc gcgctcagca accattaaac ggcggcactg
   135661 atccggtgag ggggtgtagg tggtgtcttt catccccaat ggacgggtaa tctgctcttc
   135721 aaacaactgg gtataaggct tgccggacgc attcgccagt gcatctgcca gcaggtcgaa
   135781 cgcaaggtta gagtacgccg cctgactgcc tggcgcggct tttagcttcg ccgtagaaag
   135841 gtatttccag cgttgttcgc gcgttggcca gacaaacacc ggacgatgtg ccgcgccacc
   135901 gggctgttca cggggcaggg cgctggtatg ggttgccaga ttcactaggg taatgggcgt
   135961 cccattgtag gtcggcacgc gtgcgcctgg cggagcatat ttacttagcg gatcgtttaa
   136021 ctttacggtc ccctggtcga gcaatttcac cagcatttca ctggtcatta gtttggtgag
   136081 ggaagcaata cgcacgaccg aatccagctg cgggcgaacg ttattaccag gtcgcgtttc
   136141 gccataacta cgaaagacgc gctggttacc gtcgataaca accagcgcca tccccgtggc
   136201 accgctgccg taaaaaatat gatcggcata acgatcgaca atatcagagg caaactccgg
   136261 ttcagtgatc ggctgcgccg catggacgga ggtcaatgac gccgcacaca gcacggcaga
   136321 aaaaagcaga ctacgtttca acggtggtgt ccatagattg aatgaaggta actgagcgta
   136381 tttatactac tcattgatgg attgcaaagg gcgaattagt gcaataatag tgttaaaaac
   136441 gtaacggcgc gtaaacacga tctttttcct gctcaccaat gcctgcattt gtgatcctgc
   136501 gcgtgttaat ccgcttatgt aaacaatctt tggtttgtga aatccgtcga aacaattctt
   136561 atggtcaggc tggcgaacta agcgccttgc tatgggtcac aatgggcggt ttcatttggc
   136621 tgacgctttg tagtggtcat gcggttaata cacagcagtt gttaaaacga taagaagtta
   136681 gcaggagtgc atatgtttaa gtcttttttc ccaaagccgg gagcgttttt tctctcggcc
   136741 tttgtttggg cattgattgc cgttatcttc tggcaagccg gtgggggtga ctgggtggcg
   136801 cgtatcaccg gcgcttccgg gcagatcccg attagcgccg cgcgtttctg gtcgttggat
   136861 ttcctgattt tttacgctta ctacattgtt tgcgtaggac tttttgcatt tttctggttt
   136921 atctacagcc cgcaccgttg gcaatactgg tcaatactcg gtactgcact gatcatcttc
   136981 gtcacctggt ttttgttgga agtcggggtc gccgtcaacg cctggtatgc accgttctat
   137041 gatctgattc aaaccgcgct aagttcgccg cataaagtca ctatcgaaca attttaccgc
   137101 gaagtgggcg tctttctggg gattgcgctg atcgcggtgg tgatcagtgt actgaacaac
   137161 ttctttgtca gtcactatgt gttccgctgg cgtacggcga tgaacgaata ttacatggcg
   137221 aactggcaac aactgcgtca tatcgaaggt gccgcacagc gtgtgcagga agacaccatg
   137281 cgttttgctt caacgctgga gaatatgggc gtcagtttta tcaacgccat catgacgttg
   137341 atcgcctttc tgccggtgct ggtaacgctc tccgcgcatg tgccggagct gccaattatc
   137401 gggcacattc cgtatggtct ggtgattgcc gctatcgtct ggtcgctgat ggggaccgga
   137461 ttgctggcag tggtagggat caaactgccg gggctggagt ttaaaaacca gcgagtagag
   137521 gccgcctacc gtaaagagct ggtttatggt gaagacgatg ccacgcgcgc gacgccgcct
   137581 acggtacgcg agctgtttag cgccgtacgg aaaaactatt ttcgcctcta ttttcactat
   137641 atgtatttca acatcgcccg cattctctat ttgcaggtcg ataacgtttt cggtttgttc
   137701 ttgctgtttc cgtcaattgt tgccggtacg attacgctcg gcctgatgac gcagattacc
   137761 aacgtttttg gtcaggttcg cggtgctttc cagtacctga ttaactcatg gaccacactg
   137821 gttgagttga tgtctatcta caaacgtctg cgcagctttg aacatgagct ggatggtgac
   137881 aaaattcagg aagtaaccca taccttgagc taaaaaggag aagtaatgtc gcgcgtcaat
   137941 catctatctt cgctgtcatt gttggcggtt ctggttttag cggggtgtag tagtcaggct
   138001 ccgcaaccgt tgaaaaaggg cgaaaaagcg atagatgtag cgagcgtcgt gcggcagaag
   138061 atgcctgcga gcgtgaaaga ccgcgatgcc tgggcgaaag atctcgccac cacctttgaa
   138121 agtcaggggc tggcaccgac gctggaaaac gtctgctcgg tgctggcggt ggcgcagcag
   138181 gagtcaaatt atcaggcaga tccggccgtt ccgggcttaa gcaagatcgc ctggcaagag
   138241 attgaccgtc gtgctgaacg gatgcacatt cctgcttttc tggttcatac cgcgctaaaa
   138301 atcaaatcgc caaacggcaa aagttatagc gaacggttag attcggtgcg tacagaaaag
   138361 caattgagcg cgatttttga cgatctgatc agcatggtac caatggggca gacgctgttt
   138421 ggttcgctta atcctgtgcg caccggtggg ccgatgcagg tcagcattgc ttttgccgaa
   138481 cagcatacca aagggtatcc gtggaaaatg gacggtacag ttcgtcagga agtcttcagt
   138541 cgccgcggcg ggttgtggtt tggtacttac catttactga attatcccgc cagctatagc
   138601 gcaccgatat accgctttgc tgattttaac gctggctggt acgccagccg taatgccgcg
   138661 tttcagaacg cagtcagtaa ggccagcagc gtgaagctgg cactggacgg tgatttaatt
   138721 cgttacgaca gtaaagaacc cgggaaaacc gaactggcaa cgcgcaaact ggcggcaaaa
   138781 ctggggatga gcgacagcga aattcgccgt cagttagaga aaggtgacag cttctctttt
   138841 gaggagacgg cgctgtacaa gaaagtttat caacttgccg aagcgaaaac gggtaaatca
   138901 ctaccccgcg aaatgttgcc tggcattcaa ctggaaagcc cgaaaatcac ccgcaacctg
   138961 actacggcct ggtttgcgaa gcgcgtagac gaacggcggg cgcgttgtat gaaacagtga
   139021 tcaaacagga atgtcatgcc agataaggcg ttttaggccg tatctggcaa cgtaaatctt
   139081 actggcggcg atgtcgccag tgcagccata gcgccaccac tgcaaaaatg aggcagccta
   139141 ctaaaaacgg gatcagcccg aaaatggtgc cgacacctaa accaatttcc acccgtgggc
   139201 gacctgtttc ctggacctgc attaaacgct catagacgcc gggcgcatgc actaacaggt
   139261 tcagcaattg cgccccagcc caaaagcaaa acagcacaaa caacgcatag gcaatgttgc
   139321 caggcgttga agaggcattg cgatattttc cgctaaacag cgattttgac agggtgaaat
   139381 cagccataaa aacctccgga agtcatctgc ttctcttctg gtgtgatacc agcggcttat
   139441 ggcaagtctg acaggtcatt tagattgtca atatcagaat catggtaaat tgatgttggg
   139501 aatattcccg aagcgtgaat cttcgttgtg agtcacaaaa tggcaacctt aagttaaatt
   139561 tattaacaat tacgaaaatg ttgtctacac cacagacggt tgttacagaa tatgcaagga
   139621 tgttgttttt cgttaacgga gctgccatga atctgcctgt aaaaatccgc cgtgactggc
   139681 actactatgc gttcgccatt ggccttatat tcattcttaa tggcgtggtg gggttactgg
   139741 gatttgaagc aaaaggttgg cagacctatg ccgtcggtct ggtgacgtgg gtgattagtt
   139801 tctggctggc ggggctgatt attcgtcgtc gcgatgaaga aactgaaaac gcccaataag
   139861 aatattatcg ggcgttaaaa tattacattg tggttttcaa tgcgttatcc gcagcgtgac
   139921 gctccagcgc cagctcaatc aaacgtgtga tcagatcggt gtaacccaga ccgctggctt
   139981 gccacagctt cggatacata ctgatgttgg tgaagccagg cagtgtgttg atctcgttga
   140041 tcaccacttc gttctctggg gttaaaaaca cgtctacacg cgccatgcct gcgcatccca
   140101 acgtctgata agcctgaacg gcaatcgccc ggatcttatc gttgatctct ggtgcaatgg
   140161 ctgccggaac taccactttc gcgccatctt cgtcaatgta cttggtgtcg taggcataga
   140221 aatcgctggt gagtacgatc tcgccacagg tgctggcttg cggattgtcg ttgcccagaa
   140281 ctgcgcattc gatctcacga cctttgatcc cttgctcaac gatcacttta tgatcgaact
   140341 cgaacgccag atcgacggca attgcgtact gttcttcact ggttactttg ctgacaccaa
   140401 cagaagagcc ctgattagcc ggttttacaa acagtggtaa ccccagttta gactccactt
   140461 cggcaaaact gatgttgtga cgattagcgc gcgtcagagt aataaatggc gcaatgttca
   140521 gcccggcatc gcgtagcaga cgtttggtga catctttatc catacaggct gctgaagcca
   140581 gaacatcaga acctacaaac ggtaaattgg cgacccgcag cattccctgc aaggaaccat
   140641 cttcgcccag cgtaccgtgg acaatcggga aaatgacatc caccgtcagc aacggctgac
   140701 cgttttgcgc gtcgataagc tgatgctcat gtttacctgg cacctgcgca aggctggtcg
   140761 ccgaagggcg taacgcaata tgggcaggat cgtctgcatt tagcagataa ttgctggcat
   140821 cgctgacgtg ccattgccct tgtttatcaa tgcccagcag cacaacgtcg aagcgacttt
   140881 tatcaatggc atcgacaatg ttttttgccg attgcagaga cacttcatgt tccgctgatt
   140941 taccaccaaa aacgattcct acccgcagtt tttccatctt aaaaacctat cccgtctaac
   141001 acaaagtgca tacattacca cgacaaaacg ggggattcgc ggccttctga aagattgttg
   141061 caatcttctg ctgacgaagc gtgcaacgta ctggtgaaga aagtgcgtta tctcaaagat
   141121 gtgcgcaaga tcacaaaaat gatgaacggg aagctaattt attcctggct taaatggcca
   141181 tgcggtgagt ttttttctct taattataag ttaacgaaga gaatatattt cataactttt
   141241 atttataata aaggttgata attaaaagcc tatattttgt gtgggtaatt atttaaataa
   141301 gagaaacgtt tcgctggtaa tcaaacaaaa aatatttgcg caaagtattt cctttgtcat
   141361 aaaaataata cttccagaca ctatgaagtt gtgaaacata atgttaactt ctccatactt
   141421 tggataagga aatacagaca tgaaaaatct cattgctgag ttgttattta agcttgccca
   141481 aaaagaagaa gagtcgaaag aactgtgtgc gcaggtagaa gctttggaga ttatcgtcac
   141541 tgcaatgctt cgcaatatgg cgcaaaatga ccaacagcgg ttgattgatc aggtagaggg
   141601 ggcgctgtac gaggtaaagc ccgatgccag cattcctgac gacgatacgg agctgctgcg
   141661 cgattacgta aagaagttat tgaagcatcc tcgtcagtaa aaagttaatc ttttcaacag
   141721 ctgtcataaa gttgtcacgg ccgagactta tagtcgcttt gtttttattt tttaatgtat
   141781 ttgtacatgg agaaaataaa gtgaaacaga gcactattgc actggcactc ttatcgttac
   141841 tgtttacccc tgtgacaaaa gcccggacac cagaaatgcc tgttctggaa aaccgggctg
   141901 ctcagggcga tattactgca cccggcggtg ctcgccgttt aacgggtgat cagactgccg
   141961 ctctgcgtga ttctcttagc gataaacctg caaaaaatat tattttgctg attggcgatg
   142021 ggatggggga ttcggaaatt actgccgcac gtaattatgc cgaaggtgcg ggcggctttt
   142081 ttaaaggtat cgatgcctta ccgcttaccg ggcaatacac tcactatgcg ctgaataaaa
   142141 aaaccggcaa accggactac gtcaccgact cggctgcatc agcaaccgcc tggtcaaccg
   142201 gtgtcaaaac ctataacggc gcgctgggcg tcgatattca cgaaaaagat cacccaacga
   142261 ttctggaaat ggcaaaagcc gcaggtctgg cgaccggtaa cgtttctacc gcagagttgc
   142321 aggatgccac gcccgctgcg ctggtggcgc atgtgacctc gcgcaaatgc tacggtccga
   142381 gcgcgaccag tgaaaaatgt ccgggtaacg ctctggaaaa aggcggaaaa ggatcgatta
   142441 ccgaacagct gcttaacgct cgtgccgacg ttacgcttgg cggtggcgca aaaacctttg
   142501 ctgaaacggc aaccgccggt gaatggcagg gaaaaacgct gcgtgaacag gcacaggcgc
   142561 gtggttatca gttggtcagc gatgctgcct cactgaattc ggtgacggaa gcgaatcagc
   142621 aaaaacctct gcttggactg tttgctgacg gcaatatgcc agtgcgctgg ctaggaccga
   142681 aagcaacgta ccacggcaat atcgataagc ccgcagtcac ctgtacgcct aatccgcaac
   142741 gtaatgacag tgtaccaacc ctggcgcaga tgaccgacaa agccattgaa ttgttgagta
   142801 aaaatgagaa aggatttttc ctgcaagttg aaggtgcgtc aatcgataaa caggatcacg
   142861 ctgcgaatcc ttgtgggcaa attggcgaga cggtcgatct cgatgaagcc atacaacggg
   142921 cgctggaatt cgctaaaaag gatggcaata cgctggtcat agtcaccgct gatcacgccc
   142981 acgccagcca gattgtcgcg ccggatacca aagctccggg cctcacccag gcgctaaata
   143041 ccaaagatgg cgcagtgatg gtgatgagct acgggaactc cgaagaggat tcacaagaac
   143101 ataccggcag tcagttgcgt attgcggcgt atggcccgca tgccgccaat gttgttggac
   143161 tgaccgacca gaccgatctc ttctacacca tgaaagccgc cctggggctg aaataaaacc
   143221 gcgcccggca gtgaattttc gctgccgggt ggtttttttg ctgttagcaa ccagacttaa
   143281 tggcagatca cgggcgcata cgctcatggt taaaacatga agagggatgg tgctatgaaa
   143341 ataacattac tggttacctt gctttttggt ctggtttttt taaccaccgt cggcgctgcc
   143401 gagagaacct taaccccaca acaacagcgt atgacatcct gtaatcagca ggcgacggcg
   143461 caggcgttga aaggggatgc tcgtaagacc tacatgagtg attgcctgaa gaacagcaag
   143521 tctgcgcctg gtgaaaaaag tttgacgcca cagcagcaaa agatgcgcga atgtaataat
   143581 caagcaacgc aacaatctct gaaaggtgat gatcgtaata agtttatgag tgcctgcctc
   143641 aagaaagccg cctgatacct gatagtgcta acgggtgagc tacgaaagtg gctcacccga
   143701 aatttcatac ttctgccttt agccccgtct ctataatttg ggaaaattgt ttctgaatgt
   143761 tcccaaaaat aatgaatgat gaaaactttt tcaaaaaagc ggcggcgcat ggggaggaac
   143821 ctcctttaac tcctcaaaac gaacatcagc ggtccgggct gcgcttcgcc cgtcgcgtca
   143881 gactaccccg tgcggttggc ctggctggca tgttcttacc gattgcttca acgctggttt
   143941 cgcacccgcc gccgggctgg tggtggctgg tgttggtcgg ctgggcgttc gtctggccgc
   144001 atttagcctg gcagatagcg agcagggccg tcgatccgct tagccgggaa atttacaact
   144061 taaaaaccga tgcagtatta gcgggaatgt gggtaggcgt aatgggcgta aacgtgctgc
   144121 cttccaccgc tatgttgatg attatgtgtc tgaatttgat gggggcaggc ggcccccgtc
   144181 tgtttgtcgc gggtctggtg ttgatggtgg tttcctgcct tgtcaccctc gagcttacgg
   144241 gcattaccgt gtcgttcaat agtgcgccgc tggaatggtg gctctccctt cccattattg
   144301 tcgtttatcc tctgctgttt ggctgggtca gctaccagac ggcaaccaaa ctggcggaac
   144361 ataaacgcag gttgcaggtc atgagtaccc gcgacggcat gacgggcgtg tataaccgac
   144421 gtcattggga aactatgtta cgcaatgaat ttgataactg tcggcggcat aatcgcgatg
   144481 caacgttact gattatcgat atcgaccatt tcaagagcat caacgatacc tggggtcatg
   144541 atgtgggcga tgaagcgatt gtggcgctta cccgacagtt acaaattacc ctgcgcggta
   144601 gcgatgtgat tggtcggttt ggcggcgatg agtttgcagt aatcatgtcc ggtacgccag
   144661 ctgagagcgc cattaccgcc atgttacggg tgcgtgaagg gctaaacacg ttacgcttgc
   144721 cgaatacgcc acaggtaact ttacggatta gtgtgggggt tgcgccgctg aacccacaaa
   144781 tgagtcacta tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag
   144841 ccggacgtaa ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct
   144901 gagtttttct gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc
   144961 tttctcttcc agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc
   145021 tttcagtgcc cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc
   145081 ctgagcggca aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc
   145141 catcgcttcg ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg
   145201 gtggatcatc ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac
   145261 atcagcggta tcttctgggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt
   145321 aaccagcgcg ggagtgttcg gcatggcgcg gataattttt cggtcatggc ccagcgcgcg
   145381 ggcaagttgg tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag
   145441 gctggaggtg atttcgctaa gcactttaat catgatgcca ggtttaacgg cagcaaaaat
   145501 gatgtcggcg atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc
   145561 atgcagggcg gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac
   145621 ctgaccgctg gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa
   145681 accgattttc ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct
   145741 ggcagaagca taaacagaac tatgccggaa ggcaaaagcg cgacacaata gagaattacc
   145801 caacaaagga tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta
   145861 aagagatttt gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc
   145921 agagtttacg cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg
   145981 acgttgccga taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag
   146041 atataccttt ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg
   146101 aacgttacac gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct
   146161 tacgtgccag tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg
   146221 cctttgccgc ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt
   146281 aatttattat ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga
   146341 ttttcacttt aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga
   146401 tcgctattct catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat
   146461 gacacaacct ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc
   146521 ccttgccgat tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct
   146581 caatatgacg gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga
   146641 aacggcggcg ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat
   146701 tattctgacg gaatttaatc gtcactttat gcaaaataac gggatcgtgg tttatttgtg
   146761 tgcgccagta tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc
   146821 aaccttaacg ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc
   146881 gctatatcgc gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat
   146941 ttctgaaatt cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac
   147001 ttaacgttca tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg
   147061 aagcatatat agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac
   147121 tcagagccga tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag
   147181 cgatggatgc gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc
   147241 ggaagggatt ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt
   147301 ttctaataat tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta
   147361 cattatgccg tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca
   147421 gcagtggtgc ttcacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac
   147481 aatgagtgcg tcgttggcga tcctgaccat cggcattgta cctatgcagg aagttttgcc
   147541 gctcctgacg gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt
   147601 aagtcgtgaa gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac
   147661 attattaaat gacaaccagc tggcccatgt ttcgcgtcgc aaagtggagc gtgacctgca
   147721 aggtgtggtt gaagtactcg ataatcgggg ttacgacgtc attatattaa tgagtacggc
   147781 aaacattagt agtatgactg cgcgtaatac gatctttctt gagccgtcgc ggatattgcc
   147841 accgctggtt tcctctattg ttgaagatca tcaagtgggg gttatcgttc cggttgagga
   147901 gatgctgccc gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt
   147961 gggtaacccc attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc
   148021 aaaaggggct gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt
   148081 actgcaaaaa cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc
   148141 tgcggaatta ctggtgtaat tttgcgtgac agtcagcgcc tctggcccct atagtgaagt
   148201 agatgttcaa ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc
   148261 aaagtgaaat caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg
   148321 gttgaaggcg aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc
   148381 gcgctgaatg tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg
   148441 tttaatgttc ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg
   148501 tgccgctatc tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc
   148561 ctctctttaa cccataataa tatttccttt gcttcgattt tttcaatttc cgatatattc
   148621 ataattcatc aaggttgata taaatatcag tgaagatctc caggtatttt ttgcggaact
   148681 gatgacacaa cgccccctgg agcaagttac agcgggagat ctatgaatta ctgacgccaa
   148741 cgatcaacct gcaaattcac tgtacccgtt atcccatgcg cagccaaaat ggtggaagca
   148801 ccgatttacc gcgttattgg atcacgttgg ataaaaacgt gatctgggat tacccgaaag
   148861 actttattgc aggtaacggt ggagtgcgta attttcacgg tgaaacttgc tggtatcctt
   148921 acctgacgga tatttgttca atttctgatt tattgcgtga atatattgac accccaaaag
   148981 cggagttgtt aacaaaacaa tttaccagcg ataaatgggg gttagtgaat attctgcgtg
   149041 ctgctgacag gcgaataggg atgaggcgat tagatcagtt acggcgtaaa acgcataata
   149101 ttgctgcgct aaaaattatt gcccgccgta gcgaataatt atatgcctgg tgtggcatcg
   149161 tacgccggat aagacgcggc aggcgtcgca tccggcatta aaggaaaatc agcaattaac
   149221 gttgtgcttc gccacctaat ccttcaatca ggttttgaat taacgctgcc agttcaccag
   149281 tcatcaggat gaaatcggca tcaaaacgct gggcgaaatc ttcacggtcg atatcttcgt
   149341 tttgatcgcg cagctcgtcg cagaacttca gacgcttgag cgaaccatcg tcgcacatca
   149401 caaactgaat gcgctgctgc cagtcgagcg ccagtttagt caccactttt ccggcttcga
   149461 tgtgattggt gatctcttcg ctggtcagat cttgtttctt cgcacggatc acgccgccat
   149521 cttccagcaa cgatttcagc tcggcttcat caagcaactg gaagccctgt gctgcgctac
   149581 cggagcgaac ccattcggtt agcgtcagtt caatcgggtt ttccatgctc aatggtacaa
   149641 caggtaacga ccccaggctt ttacgcagta atgccagcgt atcttccgct tttttggcgc
   149701 tggcgcagtc caccataatc aaaccattaa ccgtgtcgat ccacatcatt gtctggctaa
   149761 aacggctgaa agctcgcggc agcagagagt gcagcacttc gtctttcagc gaatcttttt
   149821 cggttttctt cagcttacgc gcctgttccg cttccagttt ggcgattttc gcttccagcg
   149881 cctgtttaat caccggagac gggaggattt tttcttcttt gcgtgcgcag ataacaattt
   149941 gaccattggc aacgtgcgtt aacgcatcgc tgtgcgatcc catcggagga acccagccca
   150001 tcttcgccat gtcctggctg ccacatgggg taaatgccat cgaggctagc tgtttttcca
   150061 tctcttctgc acgcagcgaa atctcgcggc taagacggta aaccattaaa tttttgaacc
   150121 acagcatgat aatttccacg gccttgtcgt taaatttagc gggcatgata acgaattgtc
   150181 ggcggccttg cattgccaat ccggttgtcc gtctctacgc tattgatatt gaaaaaaata
   150241 aggagagtac cgtgcgtata ggtatcgatt taggcggcac caaaactgaa gtgattgcac
   150301 tgggcgatgc aggggagcag ttgtaccgcc atcgtctgcc cacgccgcgt gatgattacc
   150361 ggcagactat tgaaacgatc gccacgttgg ttgatatggc ggagcaggcg acagggcagc
   150421 gcggaacggt aggtatgggc attcctggct caatttcgcc ctacaccggc gtggtgaaaa
   150481 atgccaattc aacctggctc aacggtcagc cattcgataa agacttaagc gcgcggttgc
   150541 agcgggaagt gcggctggca aatgacgcca actgtctggc cgtttcagaa gcagtagatg
   150601 gcgcggcagc gggagcacag acggtatttg ccgtgattat cggcacggga tgcggcgcgg
   150661 gcgtggcatt caatgggcgg gcgcatatcg gcggcaatgg cacggcaggc gagtggggac
   150721 acaatccgct accttggatg gacgaagacg aactgcgtta tcgcgaggaa gtcccttgtt
   150781 attgcggtaa acaaggttgt attgaaacct ttatttcggg cacgggattt gccacagatt
   150841 atcgtcgttt gagcggacat gcgctgaaag gcagtgaaat tatcagcctg gttgaagaaa
   150901 gcgatccggt agcggaactg gcattgcgtc gctacgagct gcggctggca aaatcgctgg
   150961 cacatgtcgt gaatattctc gatccggatg taattgtcct ggggggcggg atgagcaatg
   151021 tagaccgttt atatcaaacg gttgggcagt tgattaaaca atttgtcttc ggcggcgaat
   151081 gtgaaacgcc agtgcgtaag gcgaagcacg gtgattccag cggcgtacgc ggcgctgcgt
   151141 ggttatggcc acaagagtaa aaaaaccagg caattggcgc atcatgcctg atgcgacgct
   151201 tgccgcgtct tatcaggcct acaaaatgtg ccagaaccgc gtagggcgga taaggcgttc
   151261 acgccgcatc cggcaataag caacgcgcac cgtgacagta atctacccca gtggtttcgc
   151321 cagcaccgga ctatccgccg cttgctggcg cttatagcga ccatacagca gcaacgatga
   151381 catcgcagca aacgaaagca gggcggcagg cagcgccacg taattatatg ccagccccag
   151441 cgtcagcatc ataccaccgc aatatgcgcc gacggcgcta ccgaggttaa acgctatttg
   151501 cccacctgcg gcacctaata actctccgcc tttggcattt tgcagtaaca atatttgcag
   151561 aggtgctgaa agggcaaata atcccgcgca acaaataaaa gcaaaaataa gcgacgttgt
   151621 tttcatgccg ccgcagaaaa agagcatcag cagtgccagt acaattataa agtcagtcac
   151681 tgctgcaatg cgcagtggtg aataacgtcc tgaaatcctg ccacttagca tatttcccaa
   151741 caccatccct agcccaacta acatcataat aaaggtcatc gccgtttccg aaaaaccgga
   151801 aataaacatc atgtatggct ttacgtagct gaaccaggca aacacacctg cgttgccaaa
   151861 catcgtggcg gcgaaaatta accacggggc cgggctgcgt aaaaagtgaa attgttcgcg
   151921 cagctttcct ttcgcctcgt cgcgaatatc cggcacccaa aaatagaccg atgccatcac
   151981 cgcaatatta aaaacagcga tcaataaaaa ggtgtaacgc cagctaaatt cctgacttaa
   152041 atacgttccc agcggaatgc ccagcaaatt ggcgactgtc atcccggaaa ccatccccgc
   152101 cacggcggcg gtgacttttc cgggtttgat aatttttgat aacacgatcg cgccgacgcc
   152161 aaaaaatgcg ccatgcggaa agccggaaac caggcgacca atggcaagca tcaggtaaga
   152221 cgaagagagc gtgaacatgg cgttgccaat gacgcacaac gccaccagaa acaacaagat
   152281 atgtttgagt gagtagcggc tggaaaagag tgcgatgatt ggcgcaccga ccaccactcc
   152341 cagcgcataa tacgagatca tatgcccggc agcaggaatc gaaattccta cgttatgcgc
   152401 cagctccgtg agcacgccca taatgccaaa ttcggccatc cccaaaccaa acgtgcccag
   152461 agccaaagat aaaatgactt ttttcatacc accatcacaa ctaccagaac ggctaccaat
   152521 caccgtagcc aataaagtga tagcttgcag gataacgacc agcaaggcca agagataatt
   152581 cgcccctctg tattcattat cctgctgaat agttatttca ctgcaaacgt actttccagt
   152641 ttgctgtagc ccaggccgtt gatctttttc actttgatct gcaccggaat acgctctttc
   152701 atcgcttcta cgtggctaat cacaccgatg gttttgccac tggcgttcag ggcatccagc
   152761 gcatcaaggg cggtatccag cgtttcgcta tccagcgtgc caaaaccttc atcaaggaac
   152821 agcgagtcaa tacgtgtttt atggctgacc agatccgaaa gcgccagcgc cagcgccaga
   152881 ctaacgagga aactttcgcc gccggaaagg gtacgggtat cgcgtaccgc atctgcctgc
   152941 caggtatcaa caacctcgac ttccagcgcc tcgctggctt tgcgctgtaa cagatagcgc
   153001 ccgtgcagcc gggtaagttg ctgattagcg agatggacta aattatccag cgtcagcccc
   153061 tgggcaaact tgcggaattt atcgccctct ttggaaccta ttagcgaatt cagatatccc
   153121 cagtcctcaa cctgctgcgt catttgagca atttgctgca ttaaggtttg ttgttgctga
   153181 cggttatctg catcctgctt cagctgctgg cgaatctcgc cttgactcgt ggtgttttca
   153241 cgcaactttt ggtgagtttg cgctaactct tgctgaatct gctccaccgt cacagtgaga
   153301 gccaacccgt caggtcggtg ttgttgatgc tgtgccagcg tttctgctgt ctgagtgacc
   153361 agcgtttgcg cctgacggcg ctggttttcc agattctgct tgagctgttc cagctgcgtt
   153421 agtgtttgtt catccattag cgccgcaagg aacgcctgct gatcgtcaaa gacgctggcc
   153481 tgtagcgcgg tgtcaaactg cgcctgggct ttttgcagac tttgcgccgc cagaacatcc
   153541 tgttgctgta acgtctgctg ctggctgtgt aatgcgagac attgttcatg aacctgccgc
   153601 cagttatcca gcgccacagt ttcttcgctg tgcgggagat catcactttg cggcaacgtt
   153661 tccagaatcg gcgtcagctg ctgaatacgg ttttgcagcg cggttaattc gttctggcgt
   153721 tgctgccagc tctgcgcttc ttgctgacgt gtcgccaacc agctctcttc ttcatcttcc
   153781 tgtggcaatg tcagtgcata acccgccaat gccgttaaga gctgctgctg gcgttgttca
   153841 atttgctgtt gatactggat aatttgctga ttatgcgcgg caatctgccc ttgtaattca
   153901 tgtcgttggc tgagtaaccg cagctggcgt tcgtgctcat cttgtgcatc cagccacggt
   153961 tgaatatcgt cctgtggctg caaggtgata ttgaggctgg ccgtgacggc ttgccattgt
   154021 tgagtaagtg cttgctcatc ttgtcggagg ctttgcgctt cgttttcatc gcgctgaagc
   154081 tgctttgtta aggcgtccag ttgcccgcgc agcgccgcac cttcttcgcc gagcttttta
   154141 acttcgtttt ccagcgccag taatcgagac tgattaacac caggctccag cgcctgatac
   154201 gcctcgaccg ccgggtggct ggtggaacca caaagtggac aaggctgacc cgcctgtaac
   154261 tgtgcacgtt gagcttccag cgttttgatg cgcgcttcct gctcgcaaat ggttttcaca
   154321 tcggcaagtt gctgcgtctt ttctttataa cgctggcgca tttcgttaag tgcggcgtta
   154381 cgttgcgtct gttcttgcgt gacattctgg atagcgacct gtaactgcgc cagacgtttt
   154441 tgttggggaa caatctgtcc atgtagcgcg accaggtgct gacgcagtgg gcgttgctca
   154501 gcatgttgcg ccagggcggt agcaacttca tcggcggtta acgtcaacgt gatcgccgca
   154561 agcgcattaa gtttttgctc agcatgggtt aactgttgct gccattgccg cagatgctcg
   154621 cgatcgctgg tttgttggga gaactgcgca cgccaacccg ccagttcgtt gttccactga
   154681 cggaagcggt cgtgttcctg taaccaggta ttcaggcttt gttgctgctg ctgtaattct
   154741 gctgactgct tcgccgcgtg gtggcgaatg ctcgcgcgaa gcgccattgt gctctgtaag
   154801 cgagtattta cttcttcaat ctgctggcga gtatgcgcca gcgccgcgct gtgttctgcg
   154861 atgcgttccc agtgcggacg aagatttcgt gccggttgtg ccagactgag cgccgccagt
   154921 tgaggttgcg ctttttcttc ttcggctaac gcctgttgca aggcctgctg acggcggctg
   154981 gcttcttgct gcaattcgtc cagacgcgtt aaccagttta gtgattgttg ttcttgctgc
   155041 tgcgcggtaa gtaactgttt ttcttcgtca gtaagtacct gcaaactcgc tgtcagcgat
   155101 tgcacttgtt ccggcgtgag caacgcgacg ccgctggcct gcgcttgcag cttctccagc
   155161 tcagtgcggg ccgatttgtg ctgctcaaaa accatcgccg agatttgccc gtagatttca
   155221 gtgccggtta actcctcgag caattccgcg cgttctttgg gtttggcatt caggaaggca
   155281 gcaaattgcc cctgcgaaag cagcatcgaa cgggtgaagc gcccgtaatc cagcccggtt
   155341 aacgttgctg tcagttctag cttatctttc actttgtcgg cgagaatttt gccgtcggcg
   155401 cagcgcgcca gctcaacgcg tggcacctgc aaattaccgt cgggttggtt acgcgcccga
   155461 ttctggctcc agaatgcacg gtacgcttca cctttcactt caaactccac ctccgccaga
   155521 cattcagcgg tatcgcgggt catgagatca ttttgcgatt gtgaaacgtt agagagacgc
   155581 ggagtttcgt gatacagcgc cagacaaatg gcgtccagca gggtggtttt ccccgcaccg
   155641 gttgggccgg taatggcaaa cagcccgttg ctggcgaacg gctcgcggct gaaatcaatc
   155701 ttccattcgc cttttaatga gttcaggttt ttcaggcgca ggctgagaat tttcatgctt
   155761 cgtgttctcc ggcgagggta tgcaacgtcg tggtgaaaag atgctgcaga cgttgctgct
   155821 gcgattcatc cagttcttcc agtgccagac ggcgattgaa cacctcttcg acgctgagtt
   155881 cgctgagggt ttcacgctgt tggctggcta acacacgctc gcgctgttca cgactccggc
   155941 gtaccagcaa tacttcaaca ggcaatgatt cggttaatgc ctggattttg cgctgaatat
   156001 catgcagata ctcatcagta gtgatttcga tatccagcca gacaggcggc tcctgtgata
   156061 catcgcgcca ctgttccagc tgtgcggtaa tcgacgccag atcgcctttc agcaccgcca
   156121 tgggttgcgt taccggtacg ttcaggtttt ccacgctctc taatttgccg tttgaaaatg
   156181 tcaccagatg gacatattta ctctttccgc attcatcaaa actcagtgga atgggagagc
   156241 cgcaatagcg aacatgttcc atgccgccaa taatctgtgc gcggtgaata tgcccgagcg
   156301 cgatgtagtc ggctggtgga aagttttgtg ctggaaacgc gtccagcgtg ccaatataaa
   156361 tgtcacgcac ggcgtcactt ttactggcac ccacggtcgt taagtgtccc gtggcgatga
   156421 tgggcagagg ctgatcgccg cgcagtttgc aggcatcggc atagtgttgt tggtaataat
   156481 cggtaatcgc tgccagtaaa tgctgctgtt tttcaatacc gttaagcccc gcctggctgg
   156541 taataatgtc acgcggacgt aaaaacggaa tggggcacag cactgcgcct ggcgtcccgt
   156601 cgcgacgagg caagatttgc ggcgcatgtc cggcgctggc gaccacggtc gtattgagga
   156661 acgccatgat atcgcgcgat tcattcagcg tggcgaccga gtcatggttt cctgccagta
   156721 ccaccagatg acagccagtt tgctgtaaat tgacaacaaa acggttgtat aacgtgcggg
   156781 cgtaactggg cggcgagccg gtatcgaaaa catcaccagc aacaataatc gcatccacct
   156841 gatgggtttg tgctgtttcc agcagccagt caagaaaagc ctgatgttca gcttcgcggc
   156901 ttttactgta gaagttctgg ccgagatgcc agtctgaggt gtgaaggatg cgcataacga
   156961 ttccctggcg aaaaagcatg ggcgcgatta tacccaaaca gatgtgccat ttgctttttt
   157021 ctgcgccacg gaaatcaata acctgaagat atgtgcgacg agcttttcat aaatctgtca
   157081 taaatctgac gcataatgac gtcgcattaa tgatcgcaac ctatttatta caacagggca
   157141 aatcatggcg agacgtattc tggtcgtaga agatgaagct ccaattcgcg aaatggtctg
   157201 cttcgtgctc gaacaaaatg gctttcagcc ggtcgaagcg gaagattatg acagtgctgt
   157261 gaatcaactg aatgaaccct ggccggattt aattctcctt gactggatgt tacctggcgg
   157321 ctccggtatc cagttcatca aacacctcaa gcgcgagtcg atgacccggg atattccagt
   157381 ggtgatgttg accgccagag gggaagaaga agatcgcgtg cgcggccttg aaaccggcgc
   157441 ggatgactat atcaccaagc cgttttcgcc gaaggagctg gtggcgcgaa tcaaagcggt
   157501 aatgcgccgt atttcgccaa tggcggtgga agaggtgatt gagatgcagg gattaagtct
   157561 cgacccgaca tctcaccgag tgatggcggg cgaagagccg ctggagatgg ggccgacaga
   157621 atttaaactg ctgcactttt ttatgacgca tcctgagcgc gtgtacagcc gcgagcagct
   157681 gttaaaccac gtctggggaa ctaacgtgta tgtggaagat cgtacggtcg atgtccacat
   157741 tcgtcgccta cgtaaagcac tggagccagg cgggcatgac cgcatggtgc agaccgtgcg
   157801 cggtacagga tatcgttttt caacccgctt ttaacgcctt gctcatcgga cgcagagcag
   157861 ggcttatgat ttcttaactg gagtatctta cgtgctggaa cggctgtcgt ggaaaaggct
   157921 ggtgctggag ctgctacttt gctgcttccc ggctttcatc ctgggtgcat tttttggtta
   157981 cctgccctgg tttttgctgg catcggtaac gggactgctt atctggcatt tctggaattt
   158041 attgcgcctt tcatggtggc tgtgggtgga tcgtagtatg accccgccac cggggcgtgg
   158101 tagctgggaa ccgctactat acggcttaca ccagatgcag ctgcgaaata aaaaacgccg
   158161 ccgtgaactg ggcaatctga ttaaacgctt tcgtagcggc gcggagtcgc tgcccgacgc
   158221 agtggtgctg accactgaag agggcggtat tttctggtgt aacggtctgg cgcaacaaat
   158281 tcttggtttg cgctggccgg aagataacgg gcagaacatc cttaacctgc tgcgttaccc
   158341 ggagtttacg caatatctga aaacgcgtga tttttctcgc ccgctcaatc tggtgctcaa
   158401 caccgggcgg catctggaaa ttcgcgtcat gccttatacc cacaaacagt tgctgatggt
   158461 ggcgcgtgat gtcacgcaaa tgcatcaact ggaaggggcg cggcgtaact tttttgccaa
   158521 cgtgagccat gagttacgta cgccattgac cgtgttacag ggttacctgg agatgatgga
   158581 tgagcagccg ctggaaggcg cggtacgcga aaaagcgttg cacaccatgc gcgagcagac
   158641 ccagcggatg gaagggctgg tgaagcagtt gctgacgctg tcgaaaatag aagccgcacc
   158701 gacgcatttg ctcaatgaaa aggttgatgt gccgatgatg ctgcgcgttg ttgagcgtga
   158761 ggctcagact ctgagtcaga aaaaacagac atttaccttt gagatagata acggcctcaa
   158821 agtgtctggc aacgaagatc agctacgcag tgcgatttcg aacctggtct ataacgccgt
   158881 gaatcatact ccagaaggca cgcatatcac cgtacgctgg cagcgagtgc cgcacggtgc
   158941 cgaatttagc gttgaagata acggaccggg cattgcaccg gagcatattc cgcgcctgac
   159001 cgagcgtttt tatcgcgttg ataaagcgcg ttcccggcaa accggcggta gcggattagg
   159061 gttagcgatc gtgaaacatg cggtgaatca tcacgaaagt cgcctgaata ttgagagtac
   159121 agtaggaaaa ggaacacgtt tcagttttgt tatcccggaa cgtttaattg ccaaaaacag
   159181 cgattaatcc gcctttgtca tcttttattg ccataagcca gccgatgctg gcttattttc
   159241 tttgcagtca aaatacgggc gttagatttt acaacggttg gtgatttttt gttcgcatga
   159301 ttagccatgt ctttttcacg gaaatagtgt tttatactgg ttggtgattt cttatcgcta
   159361 tatacctctg gtttttagat ccctccttgc tttaaaacgt tataagcgtt taaattgcgc
   159421 ttcaggtgct gtcatactga ctgcattaac gcggtaaatc gaaaaactat tcttcgccgc
   159481 gcctggttgg gagtatttcc cgctaaaatt gtttaaatat accgctgtat catccccagg
   159541 gattggcaca aaaatttaac gttacaacac cacatccaca ggcagtatga tttatgaccc
   159601 atcaattaag atcgcgcgat atcatcgctc tgggctttat gacatttgcg ttgttcgtcg
   159661 gcgcaggtaa cattattttc cctccaatgg tcggcttgca ggcaggcgaa cacgtctgga
   159721 ctgcggcatt cggcttcctc attactgccg ttggtctgcc ggtactaacg gtagtggcgc
   159781 tggcaaaagt tggcggcggt gttgacagcc tcagcacgcc aatcggtaaa gtcgctggcg
   159841 tactgctggc aacggtttgt tacctggcgg tggggccgct tttcgctacg ccgcgtacag
   159901 ctaccgtttc ctttgaagtg gggattgcgc cgctgactgg tgattccgcg ctgccgctgt
   159961 ttatctacag cctggtctat ttcgctatcg ttattctggt ttcgctctat ccgggcaagc
   160021 tgctggatac cgtgggcaac ttccttgcgc cgctgaaaat tatcgcgctg gtcatcctgt
   160081 ctgttgccgc gattgtctgg ccggcgggtt ctatcagcac ggcgactgag gcttatcaaa
   160141 acgctgcgtt ttctaacggc ttcgttaacg gctatctgac catggatacg ctgggcgcaa
   160201 tggtgtttgg tatcgttatt gttaacgcgg cgcgttctcg tggcgttacc gaagcgcgtc
   160261 tgctgacccg ttataccgtc tgggctggcc tgatggcggg tgttggtctg actctgctgt
   160321 acctggcgct gttccgtctg ggttcagaca gcgcgtcgct ggtcgatcag tctgcaaacg
   160381 gcgctgctat tctgcatgct tacgttcagc acacctttgg cggcggcggt agcttcctgc
   160441 tggcggcgtt aatcttcatc gcctgcctgg taacggcagt tggcctgacc tgtgcttgtg
   160501 cagaattctt tgcccagtac gtaccgctct cttatcgtac gctggtgttt atcctcggcg
   160561 gcttctcgat ggtggtttct aacctcggct taagccagct gatccagatc tccgtaccgg
   160621 tgctgaccgc tatttatccg ccgtgtatcg cactggttgt attaagtttt acacgctcat
   160681 ggtggcataa ttcgtcccgc gtgattgctc cgccgatgtt tatcagcctg ctttttggta
   160741 ttctcgacgg gatcaaagca tctgcattca gcgatatctt accgtcctgg gcgcagcgtt
   160801 taccgctggc cgaacaaggt ctggcgtggt taatgccaac agtggtgatg gtggttctgg
   160861 ccattatctg ggatcgcgcg gcaggtcgtc aggtgacctc cagcgctcac taaatcactg
   160921 aacatttgtt ttaaccacgg ggctgcgatg ccccgtggtt ttttattgtg ttgatgggtt
   160981 aggaattgat ggaaagtaag aacaagctaa agcgtgggct aagtacccgc cacatacgct
   161041 ttatggcact gggttcagca attggcaccg ggctgtttta cggttcggca gacgccatca
   161101 aaatggccgg tccgagcgtg ttgttggcct atattatcgg tggtatcgcg gcgtatatca
   161161 ttatgcgtgc gctgggggaa atgtcggtac ataacccggc agccagctct ttctcgcgtt
   161221 atgcgcagga aaacctcggc ccgctggcag gttacattac cggctggacc tattgctttg
   161281 aaatccttat tgtcgccatc gccgatgtga ccgcttttgg tatctatatg ggtgtctggt
   161341 tcccgacggt gccgcactgg atttgggtac tgagcgtggt gctgatcatt tgcgccgtaa
   161401 acctgatgag cgtgaaggtc ttcggtgagc tggaattctg gttctcgttc tttaaagtcg
   161461 ccaccatcat catcatgatt gtcgccggtt tcggcatcat catctggggg attggcaacg
   161521 gcgggcaacc gaccggtatt cataacctgt ggagcaacgg cggcttcttc agtaacggct
   161581 ggcttgggat ggtgatgtcg ttgcaaatgg tgatgtttgc ttacggtggg atcgaaatta
   161641 tcgggattac cgccggtgaa gcgaaagatc ctgaaaaatc gataccgcgt gcgattaact
   161701 ccgtgccgat gcgtattctg gtgttctacg tcggtacgct gttcgtcatt atgtctatct
   161761 acccgtggaa tcaggttggc actgccggta gcccgttcgt gctgacgttc cagcatatgg
   161821 gcattacctt tgccgccagc attcttaact ttgttgtgct gactgcttcg ctgtcggcaa
   161881 ttaacagtga cgtatttggc gtaggccgta tgctccacgg tatggcagag cagggcagcg
   161941 cgccgaaaat tttcagcaaa acgtcgcgtc gcggtattcc gtgggttacg gtgctggtga
   162001 tgactaccgc gctgctgttt gcggtgtatc tgaactacat catgccggaa aacgtcttcc
   162061 tggtgatcgc ttcgctggca accttcgcca cggtgtgggt gtggattatg atcctgctgt
   162121 cgcaaattgc cttccgtcgc cgtttgccgc cagaagaagt taaggcgctg aaatttaaag
   162181 tgccgggtgg ggtagcaacg accatcggcg gtttgatttt cctgctcttt attatcgggt
   162241 tgattggtta tcacccggat acgcgtatct cgctgtatgt cggtttcgcg tggattgttg
   162301 tgctgttgat tggctggatg tttaaacgcc gccacgatcg tcagctggct gaaaaccagt
   162361 aatccttgcg ttctgtaagc cggttcagga ggaaatcctg atccggctat tccgaaagtt
   162421 atccgccccc gtcctcctcc cccaaatatc cttcagatga tgagtgatcc tgcattaggc
   162481 tatggcaagg tgatcagatt ttcatcacag gggaattatg atgttaaatg catggcacct
   162541 gccggtgccc ccatttgtta aacaaagcaa agatcaactg ctcattacac tgtggctgac
   162601 gggcgaagac ccaccgcagc gcattatgct gcgtacagaa cacgataacg aagaaatgtc
   162661 agtatcgatg cataagcagc gcagtcagca gcagcctggc gtcaccgcat ggcgtgcggc
   162721 gattgatctc tccagcggac aaccccggcg gcgttacagt ttcaaactgc tgtggcacga
   162781 tcgccagcgt tggtttacac cgcagggctt cagccgaatg ccgccggcac gactggagca
   162841 gtttgccgtc gatgtaccgg atatcggccc acaatgggct gcggatcaga ttttttatca
   162901 gatcttccct gatcgttttg cgcgtagtct tcctcgtgaa gctgaacagg atcatgtcta
   162961 ttaccatcat gcagccggac aagagatcat cttgcgtgac tgggatgaac cggtcacggc
   163021 gcaggcgggc ggatcaacgt tctatggcgg cgatctggac gggataagcg aaaaactgcc
   163081 gtatctgaaa aagcttggcg tgacggcgct gtatctcaat ccggtgttta aagctcccag
   163141 cgtacataaa tacgataccg aggattatcg ccatgtcgat ccgcagtttg gcggtgatgg
   163201 ggcgttgctg cgcttacgcc acaatacgca gcagctggga atgcggctgg tgctggatgg
   163261 cgtgtttaac cacagtggcg attcccatgc ctggtttgac aggcacaatc gtggcacggg
   163321 gggagcttgt cacaaccctg aatcgccctg gcgcgactgg tactcgttta gtgatgatgg
   163381 cacggcgctc gactggcttg gctatgccag cttgccgaag ctggattatc agtcggaaag
   163441 tctggtgaat gaaatttatc gcggggaaga cagtattgtc cgccattggc tgaaagcgcc
   163501 gtggagtatg gacggctggc ggctggatgt ggtgcatatg ctgggggagg cgggtggggc
   163561 gcgcaataat atgcagcacg ttgctgggat caccgaagcg gcgaaagaaa cccagccgga
   163621 agcgtatatt gtcggcgaac attttggcga tgcacggcaa tggttacagg ccgatgtgga
   163681 agatgccgcc atgaactatc gtggcttcac attcccgttg tggggatttc ttgccaatac
   163741 cgatatctct tacgatccgc agcaaattga tgcccaaacc tgtatggcct ggatggataa
   163801 ttaccgcgca gggctttctc atcaacaaca attacgtatg tttaatcagc tcgacagcca
   163861 cgatactgcg cgatttaaaa cgctgctcgg tcgggatatt gcgcgcctgc cgctggcggt
   163921 ggtctggctg ttcacctggc ctggtgtacc gtgcatttat tacggtgatg aagtaggact
   163981 ggatggcaaa aacgatccgt tttgccgtaa accgttcccc tggcaggtgg aaaagcagga
   164041 tacggcgtta ttcgcgctgt accagcgaat gattgcgctg cgtaagaaaa gtcaggcgct
   164101 gcgtcgtggc ggctgtcagg tgctgtatgc ggaagataac gtagtggtat ttgtccgcgt
   164161 gctgaatcag caacgtgtac tggtggcaat caaccgtggc gaggcctgtg aagtggtgct
   164221 ccccgcgtca ccgtttctta atgtcgcgca atggcaacgc aaagaaggcc atgggcaact
   164281 gacaaacggg attctggctt tgcctgccat ttcggctacg gtatggatga actaacgttt
   164341 tataacgcct tgtgtgacgc ctgcgccatc atccgcggat aaaactgcca gaagcgggtt
   164401 tcgagggcgt cataatgagc gtctaaatcg taccaggagt cacgcagggc atccagacgt
   164461 gggcggcggc ttgccatgcc gtttaacacg ttctggatga aatccatatc gcgatagcgc
   164521 accagccact gctctgacca caagtaattg ttcagattga taaaacgtgg cggtgagtcc
   164581 ggcaaaatcg tcatcacttg ctcgcgggca taacagacaa attcctgtag cggaaagtcc
   164641 ggcgacagct gcgaccagtg gcgggaaaga aagtgatccc acatgacatc cagcgtaata
   164701 ggcgcaacgc ggcgagtttc actacgaaac cattcccgtg cttcgcggac ttccggcaga
   164761 ttgtcggtta atacgtcgat acgtcgatgc atatgaatgc cagccacgac gtcgggcgga
   164821 aaactttctt caggatttcc gcgtacgaaa tcagccagta aattgccgga aagcgagctt
   164881 tccgcgagat gggctaaatg caggtgagct aaaaaattca tcgattatat tctatccaaa
   164941 agggggtaaa ggttgcaggg agagcgcccc ggcactagac tacccgcctc ttattttagt
   165001 ctgagtcagt gtcatgcgcg ttaccgattt ctcctttgaa ttgcccgaat ccctgattgc
   165061 ccactatccc atgcctgaac gcagtagctg tcgtttactg tcgctggacg ggccgacggg
   165121 cgcgctgacg cacggtactt tcaccgattt acttgataag ctcaaccccg gcgatcttct
   165181 ggtttttaat aatacccgcg tgatcccggc gcgcctgttt gggcgtaaag ccagcggcgg
   165241 caagattgaa gtgctggttg aacggatgct cgacgacaaa cgcattcttg cgcatattcg
   165301 cgcctcgaaa gcgccaaaac ctggcgcaga actgctgctg ggcgatgacg aaagtattaa
   165361 cgcaacaatg accgcgcgcc acggcgcact gtttgaagtc gaatttaatg atgaacgctc
   165421 ggtgctggat attctcaaca gcatcggcca tatgccgctg ccgccgtata tcgaccgtcc
   165481 ggacgaagac gctgaccgcg aactttatca aaccgtttat agcgaaaaac cgggcgcggt
   165541 tgcagccccg accgcaggtc tgcattttga cgaacctttg ctggaaaaat tacgcgccaa
   165601 aggcgtggag atggcgtttg tgacgttgca cgttggtgcg ggcaccttcc agccggtgcg
   165661 cgtcgacacc attgaagatc acatcatgca ctcggaatac gctgaagtgc cgcaggatgt
   165721 ggtagacgcg gtactggcgg cgaaagcgcg cggtaaccgg gtgattgcgg ttggcaccac
   165781 ctcagtacgt tcgctggaaa gcgcggctca ggcggcgaaa aacgatctta tcgaaccgtt
   165841 ctttgacgat acccaaatct ttatctatcc gggcttccag tacaaagtgg tcgatgcgct
   165901 ggtgacaaac ttccacctgc cggaatcgac gctgattatg ctggtttcgg cctttgccgg
   165961 ttatcaacac accatgaatg cctataaagc agcggtagaa gagaaatatc gcttttttag
   166021 ttacggtgat gcgatgttta tcacgtacaa tccgcaggca attaatgagc gcgtcgggga
   166081 gtaattccgc ggcgctggtt taaaacgttg gactgttttt ctgacgtagt ggagaaaaaa
   166141 tgaaatttga actggacacc accgacggtc gcgcacgccg tggccgcctg gtctttgatc
   166201 gtggcgtagt ggaaacgcct tgttttatgc ctgttggcac ctacggcacc gtaaaaggga
   166261 tgacgccgga agaagttgaa gccactggcg cgcaaattat cctcggcaac accttccacc
   166321 tgtggctgcg cccgggccag gaaatcatga aactgcacgg cgatctgcac gattttatgc
   166381 agtggaaggg gccgatcctc accgactccg gcggtttcca ggtcttcagc cttggcgata
   166441 ttcgtaaaat caccgaacag ggcgtgcact tccgtaaccc gatcaacggc gatccgattt
   166501 tcctcgatcc tgaaaaatca atggagattc agtacgatct tggttcggat atcgtcatga
   166561 tctttgatga gtgtacgccg tatcctgctg actgggatta cgcaaaacgc tccatggaga
   166621 tgtctctgcg ttgggcgaag cgtagccgtg agcgttttga cagtctcgga aacaaaaatg
   166681 cgctgtttgg tatcattcag ggcagcgttt acgaagattt acgtgatatt tctgttaaag
   166741 gtctggtaga tatcggtttt gatggctacg ctgtcggcgg tctggctgtg ggtgagccga
   166801 aagcagatat gcaccgcatt ctggagcatg tatgcccgca aattccggca gacaaaccgc
   166861 gttacctgat gggcgttggt aaaccagaag acctggttga aggcgtacgt cgtggtatcg
   166921 atatgtttga ctgcgtaatg ccaacccgca acgcccgaaa tggtcatttg ttcgtgaccg
   166981 atggcgtggt gaaaatccgc aatgcgaagt ataagagcga tactggccca ctcgatcctg
   167041 agtgtgattg ctacacctgt cgcaattatt cacgcgctta cttgcatcat cttgaccgtt
   167101 gcaacgaaat attaggcgcg cgactcaaca ccattcataa ccttcgttac taccagcgtt
   167161 tgatggcggg tttacgcaag gctattgaag agggtaaatt agagagcttc gtaactgatt
   167221 tttaccagcg tcaggggcga gaagtaccac ctttgaacgt tgattaatat taataatgag
   167281 ggaaatttaa tgagcttttt tatttctgat gcggtagcgg caacgggtgc accggcgcaa
   167341 ggtagcccga tgtctttgat tttgatgctg gtggtattcg gtctgatttt ctatttcatg
   167401 atcctgcgtc cacagcagaa gcgcaccaaa gaacacaaaa agctgatgga ctccattgcc
   167461 aaaggtgatg aagttctgac gaacggtggc ctggttggtc gcgtaaccaa agtagcggaa
   167521 aacggctaca ttgctatcgc gctgaatgac accactgaag tagttattaa acgtgacttc
   167581 gtagctgccg tcctgccgaa aggcaccatg aaggcgctgt aattaaaatt tttccctaag
   167641 ggaattgccg tgttaaaccg ttatcctttg tggaagtacg tcatgctgat cgtggtgatt
   167701 gtcatcggtc tgctgtatgc gcttcccaac ctgtttggtg aggatccggc tgttcagatc
   167761 actggtgcgc gcggagtcgc cgccagtgag caaacgctga tccaggtcca gaaaacgtta
   167821 caagaagaaa aaataactgc taagtctgtg gcactggaag agggcgctat tcttgcgcgc
   167881 ttcgactcca ctgacaccca gctgcgcgct cgtgaagcat taatgggcgt tatgggtgac
   167941 aaatacgtcg tggcgcttaa ccttgccccg gcaacgccgc gctggctggc agctattcac
   168001 gctgagccga tgaagctcgg ccttgacctg cgtggcggcg ttcacttcct gatggaagtg
   168061 gatatggaca ccgcgcttgg caaactccag gaacaaaata tcgatagcct gcgcagcgat
   168121 ctgcgtgaaa agggcatccc gtataccacc gttcgtaaag aaaacaacta cggcctgagc
   168181 attactttcc gcgatgctaa agctcgtgat gaagccattg cgtatctgag caagcgccat
   168241 ccggacctgg tgattagtag ccagggcagc aaccagctgc gtgcggtaat gagcgatgct
   168301 cgtctgagtg aagcgcgtga atatgcggtg cagcagaaca tcaatatcct gcgtaaccgt
   168361 gtaaaccaac ttggcgtggc ggagccggtg gttcagcgtc agggtgctga ccgtatcgtt
   168421 gttgaactgc caggtattca ggacactgcg cgtgcgaaag agattctggg tgcgacggca
   168481 acgctggaat tccgtctggt aaacaccaac gttgaccagg ccgctgcggc atccggtcgc
   168541 gtaccgggcg actctgaagt gaaacagacc cgcgaaggtc agccagttgt gctgtacaaa
   168601 cgcgtaattc tgaccggtga ccatatcacc gactccactt ccagccagga cgaatacaac
   168661 caaccgcagg ttaacatctc gctcgatagc gctggtggta acatcatgtc taacttcact
   168721 aaggacaaca tcggcaaacc gatggcaacc ctgtttgtgg agtacaaaga cagcggtaag
   168781 aaagatgcaa atggtcgtgc ggttctggtg aaacaggaag aggtgattaa catcgccaac
   168841 atccagtctc gtctgggtaa cagcttccgt atcaccggca tcaacaaccc gaacgaagcc
   168901 cgtcagctgt cactgctgct gcgtgccggt gcgttgatcg cgccgattca gattgttgaa
   168961 gaacgtacca ttggcccaac cctgggtatg cagaacattg aacaggggct ggaggcttgc
   169021 cttgccggtc tgctggtttc tattctgttc atgatcatct tctataagaa gtttggtctg
   169081 attgcgacca gtgctctgat tgccaacttg atcttaatcg tcggcattat gtcgctgttg
   169141 ccaggcgcaa cgctgagtat gccaggtatc gcgggtatcg tcttaaccct tgcggtggcg
   169201 gtcgatgcga acgtactgat caacgaacgt attaaagaag agttgagcaa cggacgtact
   169261 gttcaacagg caattgatga aggttatcgt ggcgcattca gttctatctt cgatgcgaac
   169321 atcaccacgc tgattaaagt catcatcctg tacgcagtgg gtaccggggc aattaaaggg
   169381 ttcgcgatta ctaccggtat cggtgtggcg acgtcgatgt ttaccgcgat tgtcggtacg
   169441 cgtgccatcg taaacctgct atatggcggc aagcgcgtca agaagctgtc aatctgagga
   169501 gtgcgatgtg gcacaggaat atactgttga acaactaaac cacggccgta aagtctatga
   169561 ctttatgcgc tgggactact gggctttcgg catctctggt ctgctgttaa tcgccgctat
   169621 cgttattatg ggcgtgcgcg gctttaactg ggggctggat ttcaccggtg gtacggttat
   169681 cgaaattacg ctcgaaaaac cggctgaaat tgacgtaatg cgtgatgcat tgcaaaaagc
   169741 cggttttgaa gagccaatgc tgcaaaactt tggtagcagc cacgacatca tggtccgtat
   169801 gccgcctgct gaaggcgaaa ccggcggtca ggtgctgggc agccaggttc tgaaggtgat
   169861 taacgaatcc accaatcaga atgcagcagt gaagcgtatt gagttcgtcg gtccgagcgt
   169921 gggggcagac cttgcgcaaa ccggtgcgat ggcgttgatg gcagcgctgc tgtctatcct
   169981 cgtgtacgta ggtttccgct ttgagtggcg actggcggca ggggtggtta ttgcgctggc
   170041 gcacgacgtt atcattacgc tgggtatttt gtcgttattc catatcgaga ttgacctgac
   170101 cattgtggca tcgttgatgt cggttatcgg ttactcgctt aacgacagta tcgtggtatc
   170161 ggaccgtatt cgtgaaaact tccgcaagat ccgtcgcggt acgccttacg aaatctttaa
   170221 cgtgtccttg acccagacgc tgcaccgtac cttgatcaca tccggtacta ccttgatggt
   170281 tatcctgatg ctgtacctct tcggtggtcc ggtactggaa ggcttctcgc tgaccatgct
   170341 tatcggtgtt tccatcggta ctgcatcttc catctatgtg gcatctgcgt tggctctgaa
   170401 actgggtatg aagcgcgaac acatgttgca gcagaaagtg gaaaaagaag gggcggatca
   170461 gccgtcaatt ctgccttaat caagttcccg ttgatgttga aaatcccggt cagaagatcg
   170521 ggattttttt gatgtatgga gattgcgaaa atccccgcat cttgggaaac tgcgcgtaac
   170581 cctacatttc atccaggtaa cttttcatgg ctatcatccc aaaaaactat gcgcggttag
   170641 aaagcggcta tcgcgaaaaa gcattaaaaa tctatccgtg ggtctgcggt cgctgttccc
   170701 gcgagtttgt ctattccaac ctgcgtgaac ttactgttca ccacattgat cacgaccata
   170761 ccaataaccc ggaagatggc agtaactggg aattgctttg tctctattgc cacgatcatg
   170821 agcattcgaa atataccgaa gcggatcagt atggtacgac cgttatcgca ggggaagatg
   170881 cgcagaaaga tgtcggtgaa gcgaagtaca acccattcgc tgacctgaaa gcgatgatga
   170941 acaagaagaa gtgattaaaa cgtaaaattg cctgatgcgc tacgcttatc aggactacgt
   171001 tatttcagca atatattgaa tttacgtgct tttgtgggtc ggacaaagcg tttacgccgc
   171061 atccggcatg aacaaagcgc acgttgttaa caatctgaaa tgccgggaat aaatcccggc
   171121 attttcataa tcagaagttg taacctacaa ccaggtaacc accccagccg gtagagcgaa
   171181 cgttgaagtt gccgttgccg aagttcagtt ctgcatcgtc gttccactga ccaccgtcgt
   171241 gccagtaacg agctacgaca gagtagtgcc agtgatcgta gttcagagcc agaatatggc
   171301 tggaagcgat agagttatta gtacgggtct taataccgtt gattgcgtta ccgctgtcat
   171361 cccctaaatc ggaaccccag tcgaagttgg tgaagccgat gtagctcagc tgaccgcccc
   171421 acagatcggt aatcggcaca aagtatttaa ttttgaaacg gtaaccgtcc cactcgtttt
   171481 cgttcgctgc gccatagttc tgccactggt atttcgcata gacgttcatg gacaggctca
   171541 tcggcagacc agtatcgatg tcggtaccca gacccatata ccaggtgctc tggcgaccat
   171601 ctttattacg acccatgtcg taaatgtagt tgttcgcgaa gtaccactct ttgaacggac
   171661 cgaagctaag gtcagtattg gtcagcttgt cgatggagaa acgtggttcg atttccataa
   171721 acagcggaga gccgtggttc cagatacctt tggcatcgga gttaccgccg aagaataccg
   171781 gcgcatccgc ataaccatag aagtcgaacc agtctttttt agcgaatgct tcgtactcaa
   171841 ggtaggtatc gttgcggatc tgcggtccaa aacgggtgtg atagcttccg acaacgttaa
   171901 cgctctggtg ccaccagtcg gaaagatact gcggtttgtc gttttcagct gcgttgacag
   171961 taaaagacga agagagcgcc agtaccgcac cggctgccag taatgttttt ttcatatgta
   172021 tgccactgtt tgaaaatccc ttgcgggagt gaaaaaggcg caaattgcgt ttctaaatat
   172081 ttcgtataca gaggagccta ttataaaaat cattgctcac aaaaatatgt ttcgtttcac
   172141 agttctatca tttacgtaat cgattgcgtt cacgtttaca cacattcggg cggggattgt
   172201 actgactttc gcccttgttg caaactttta ctatcaatcc aggtgatgta agcagagtaa
   172261 caaaatgacg gggaaatatg ttgcgggacg cacaaaatcg tcccgaaata cgtttactgc
   172321 ataacaggtt gaatgtcgtg gatacgtaca aagcctaact gatccacaga aatccccttt
   172381 aattgtaacg gaatatcaac atcgctgggg gcgaggacgc tggcaggggc attaatcaat
   172441 tgattctgca cattgatttc ctgaaagttg tctattgttc cctgaatttg cccatattca
   172501 acagttccgc tgaatgcggg caagggatca ttcgactcgc cctgaatacg tagcgtcagg
   172561 gtggtgccat ctgcatcagg cgtaatattc accagcgaca tacgtaaagt accgatctgg
   172621 ctttccagtc ttgccggtgt tttcgctcca ggcagaaggt aaaccccact gctggatttt
   172681 gcattcagcc tgttttgctg agtgattttg accgtttctt tattgagttg cgtcatctcc
   172741 tggttcagcg tgctgacgct gtgtttcatc tgtcgcactt cgctctgctg cacacaggcg
   172801 ctaaggctga acaggcttcc cagcaggagc agtcgaaaaa cgttagtatt cataggtcgt
   172861 ccctttctcg tcgcaattag cctaatggta gagggtaaaa gcagcgaaag catcgatcct
   172921 ttatctcaaa agcgttgcgc ctttgttgta tcgtcagttc agggtaaaat agatttctgt
   172981 taaccacctg gtcaggacgc cgtatgcatt gcccattctg tttcgccgtg gacactaagg
   173041 taattgactc tcgtctcgtg ggcgagggtt catccgtacg ccgccgtcgg cagtgtctgg
   173101 tgtgtaatga acgtttcacc acctttgaag tggcggagct ggttatgccg cgtgttgtaa
   173161 aaagcaacga cgtgcgtgaa ccgtttaatg aagagaaatt gcgtagcgga atgctgcggg
   173221 cgctggaaaa acgtccggtg agttccgatg acgtcgaaat ggcaatcaat catattaaat
   173281 cgcagctgcg cgccaccggt gagcgcgaag taccgagcaa gatgattggc aatctggtga
   173341 tggagcaatt gaaaaagctc gataaagtcg cctatatccg ttttgcctct gtctaccgca
   173401 gtttcgaaga tatcaaagaa tttggcgaag agatcgcgcg cctggaggac taagccgtgc
   173461 aggacgagta ttacatggcg cgggcgctaa agctggcgca acgaggacgt tttaccacgc
   173521 atcccaaccc gaatgtcggg tgcgtcattg tcaaagatgg cgaaattgtc ggtgaaggtt
   173581 accaccaacg tgcgggtgaa ccacatgccg aagtacacgc gttgcgtatg gcgggtgaaa
   173641 aagccaaagg tgcgaccgcc tatgtcacac tcgaaccctg tagccatcat ggtcgtacgc
   173701 caccgtgctg tgacgcactc atcgccgctg gcgtagcgcg cgtggttgcc tcgatgcaag
   173761 atcctaaccc gcaggtcgct gggcgtggac tttaccgtct gcaacaggct ggcattgacg
   173821 tcagccacgg gctgatgatg agtgaagccg agcaattgaa taaaggcttt ctcaagcgga
   173881 tgcgcaccgg ctttccttat attcagttaa aacttggcgc atcgcttgat ggccgcacgg
   173941 cgatggcgag cggcgaaagc cagtggatca cttcgcctca ggcgcggcgc gacgtacaac
   174001 gacagcgcgc acaaagtcat gccattttaa ccagcagcgc cacggtgctg gcggatgatc
   174061 cagccttaac agtgcgttgg tctgaactgg atgaacaaac tcaggcgctc tacccgcaac
   174121 aaaatctccg ccagccagta cgtattgtga ttgatagcca aaatcgcgtg acgccggaac
   174181 atcgcattgt gcagcagccc ggcgaaacct ggttcgcgcg tacgcaggaa gattctagtg
   174241 agtggccgga aacggtgcgt accttgctga ttccagagca taaaggtcat ctggatctgg
   174301 ttgtactgat gatgcaactg ggtaaacagc aaattaacag catctgggtg gaagcggggc
   174361 caacgctcgc tggcgcattg ctgcaggcgg gtttagtcga tgagctgatt gtctatatcg
   174421 cacctaaact attaggcagc gacgcccgcg gattatgcac gctgccaggg cttgagaaat
   174481 tagccgacgc cccccaattt aaattcaaag agatacgtca tgtaggcccg gatgtttgcc
   174541 tgcatttagt gggtgcatga tctcccggct cgaaagggaa gcagcgcacg aaatattatg
   174601 ctaaaatccg cccccctgcg gggccatact cgaacccgaa ggaagaaaat gaacattatt
   174661 gaagctaacg ttgctacccc ggacgctcgc gtcgccatca ccatcgcgcg tttcaacaac
   174721 tttatcaatg acagcctgct ggaaggtgca attgacgcac tgaaacgtat cggtcaggta
   174781 aaagatgaaa acattaccgt tgtttgggtg cctggtgcct atgagctgcc gctggcggcg
   174841 ggtgcactgg ctaaaaccgg taaatacgac gcggtgattg cgctgggtac ggttattcgt
   174901 ggtggcactg cccactttga atatgtcgct ggtggtgcaa gcaacggcct ggcgcatgtt
   174961 gcccaggaca gcgaaattcc ggttgctttt ggggttctga ccactgaaag cattgaacaa
   175021 gcgatcgaac gtgctggcac caaagctggc aacaaaggtg cagaagctgc actgaccgcg
   175081 cttgaaatga ttaatgtatt gaaagccatc aaggcctgaa attagtaagg ggaaatccgt
   175141 gaaacctgct gctcgtcgcc gcgctcgtga gtgtgccgtc caggcgctct actcctggca
   175201 gttgtcccag aacgacatcg ctgatgttga ataccagttc ctggctgaac aggatgtaaa
   175261 agacgttgac gtcctgtact tccgtgagct gctggccggg gtggcgacta acaccgcata
   175321 cctcgacgga ctgatgaagc catacctgtc ccgcctgctg gaagaactgg gacaggtaga
   175381 aaaagcagta ctgcgcattg cgctgtacga gctgtctaaa cgtagcgatg tgccatacaa
   175441 agtggccatt aacgaagcga tcgaactggc gaaatcgttc ggcgcagaag acagccataa
   175501 gttcgtcaac ggc
//
LOCUS       DAFATA010000003       127366 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFATA010000003 DAFATA010000000
VERSION     DAFATA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 127366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 127366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..127366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.003"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..428)
                     /gene="opgD"
                     /locus_tag="LGJ20_000344"
                     /gene_synonym="mdoD"
     CDS             complement(<1..428)
                     /gene="opgD"
                     /locus_tag="LGJ20_000344"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBK2384471.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDA"
     gene            complement(653..1996)
                     /locus_tag="LGJ20_000345"
     CDS             complement(653..1996)
                     /locus_tag="LGJ20_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK2384472.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            2213..3136
                     /locus_tag="LGJ20_000346"
     CDS             2213..3136
                     /locus_tag="LGJ20_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2384473.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(3174..4814)
                     /gene="trg"
                     /locus_tag="LGJ20_000347"
     CDS             complement(3174..4814)
                     /gene="trg"
                     /locus_tag="LGJ20_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBK2384474.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVIRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            5213..5362
                     /gene="hokB"
                     /locus_tag="LGJ20_000348"
     CDS             5213..5362
                     /gene="hokB"
                     /locus_tag="LGJ20_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBK2384475.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(5434..5607)
                     /locus_tag="LGJ20_000349"
     CDS             complement(5434..5607)
                     /locus_tag="LGJ20_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384476.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(5852..6382)
                     /gene="cybB"
                     /locus_tag="LGJ20_000350"
     CDS             complement(5852..6382)
                     /gene="cybB"
                     /locus_tag="LGJ20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBK2384477.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            6571..7572
                     /gene="gap"
                     /locus_tag="LGJ20_000351"
     CDS             6571..7572
                     /gene="gap"
                     /locus_tag="LGJ20_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK2384478.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(7614..9053)
                     /gene="aldA"
                     /locus_tag="LGJ20_000352"
     CDS             complement(7614..9053)
                     /gene="aldA"
                     /locus_tag="LGJ20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBK2384479.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPTNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(9250..10050)
                     /locus_tag="LGJ20_000353"
     CDS             complement(9250..10050)
                     /locus_tag="LGJ20_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK2384480.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(10322..14224)
                     /gene="hrpA"
                     /locus_tag="LGJ20_000354"
     CDS             complement(10322..14224)
                     /gene="hrpA"
                     /locus_tag="LGJ20_000354"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBK2384481.1"
                     /translation="MTEQQKLTFTALQQRLDSLMPRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGVKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            14425..15030
                     /gene="azoR"
                     /locus_tag="LGJ20_000355"
     CDS             14425..15030
                     /gene="azoR"
                     /locus_tag="LGJ20_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK2384482.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(15081..16397)
                     /locus_tag="LGJ20_000356"
     CDS             complement(15081..16397)
                     /locus_tag="LGJ20_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBK2384483.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(16387..18144)
                     /locus_tag="LGJ20_000357"
     CDS             complement(16387..18144)
                     /locus_tag="LGJ20_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBK2384484.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISHLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRMAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALENEPAVCDILLRDYSELNVAQGQE
                     MIAQRGMSGQVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(18160..19056)
                     /locus_tag="LGJ20_000358"
     CDS             complement(18160..19056)
                     /locus_tag="LGJ20_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK2384485.1"
                     /translation="MLEKSLATLFTLLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLISATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMFPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            complement(19056..19661)
                     /locus_tag="LGJ20_000359"
     CDS             complement(19056..19661)
                     /locus_tag="LGJ20_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK2384486.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNALLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVSIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(19832..22137)
                     /locus_tag="LGJ20_000360"
                     /pseudo
     CDS             complement(19832..22137)
                     /locus_tag="LGJ20_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
     gene            complement(22201..23061)
                     /gene="pdxI"
                     /locus_tag="LGJ20_000361"
     CDS             complement(22201..23061)
                     /gene="pdxI"
                     /locus_tag="LGJ20_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HBK2384487.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(23292..23882)
                     /gene="paaY"
                     /locus_tag="LGJ20_000362"
     CDS             complement(23292..23882)
                     /gene="paaY"
                     /locus_tag="LGJ20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HBK2384488.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(23864..24814)
                     /gene="paaX"
                     /locus_tag="LGJ20_000363"
     CDS             complement(23864..24814)
                     /gene="paaX"
                     /locus_tag="LGJ20_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HBK2384489.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(24915..26228)
                     /gene="paaF"
                     /locus_tag="LGJ20_000364"
     CDS             complement(24915..26228)
                     /gene="paaF"
                     /locus_tag="LGJ20_000364"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HBK2384490.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(26255..27460)
                     /gene="paaJ"
                     /locus_tag="LGJ20_000365"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(26255..27460)
                     /gene="paaJ"
                     /locus_tag="LGJ20_000365"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HBK2384491.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(27460..27882)
                     /gene="paaI"
                     /locus_tag="LGJ20_000366"
     CDS             complement(27460..27882)
                     /gene="paaI"
                     /locus_tag="LGJ20_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HBK2384492.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(27872..29299)
                     /gene="paaC"
                     /locus_tag="LGJ20_000367"
     CDS             complement(27872..29299)
                     /gene="paaC"
                     /locus_tag="LGJ20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HBK2384493.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(29301..30089)
                     /gene="paaB"
                     /locus_tag="LGJ20_000368"
     CDS             complement(29301..30089)
                     /gene="paaB"
                     /locus_tag="LGJ20_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HBK2384494.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(30089..30856)
                     /locus_tag="LGJ20_000369"
     CDS             complement(30089..30856)
                     /locus_tag="LGJ20_000369"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HBK2384495.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(30853..31923)
                     /gene="paaK"
                     /locus_tag="LGJ20_000370"
     CDS             complement(30853..31923)
                     /gene="paaK"
                     /locus_tag="LGJ20_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HBK2384496.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(31931..32428)
                     /gene="paaD"
                     /locus_tag="LGJ20_000371"
     CDS             complement(31931..32428)
                     /gene="paaD"
                     /locus_tag="LGJ20_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HBK2384497.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(32443..33189)
                     /gene="paaC"
                     /locus_tag="LGJ20_000372"
     CDS             complement(32443..33189)
                     /gene="paaC"
                     /locus_tag="LGJ20_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HBK2384498.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(33198..33485)
                     /gene="paaB"
                     /locus_tag="LGJ20_000373"
     CDS             complement(33198..33485)
                     /gene="paaB"
                     /locus_tag="LGJ20_000373"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HBK2384499.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(33497..34426)
                     /gene="paaA"
                     /locus_tag="LGJ20_000374"
     CDS             complement(33497..34426)
                     /gene="paaA"
                     /locus_tag="LGJ20_000374"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HBK2384500.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            34711..36756
                     /gene="paaZ"
                     /locus_tag="LGJ20_000375"
     CDS             34711..36756
                     /gene="paaZ"
                     /locus_tag="LGJ20_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HBK2384501.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDVLVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            37004..39277
                     /gene="tynA"
                     /locus_tag="LGJ20_000376"
     CDS             37004..39277
                     /gene="tynA"
                     /locus_tag="LGJ20_000376"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HBK2384502.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQSLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGAVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(39335..40834)
                     /gene="feaB"
                     /locus_tag="LGJ20_000377"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(39335..40834)
                     /gene="feaB"
                     /locus_tag="LGJ20_000377"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HBK2384503.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            41070..41975
                     /gene="feaR"
                     /locus_tag="LGJ20_000378"
     CDS             41070..41975
                     /gene="feaR"
                     /locus_tag="LGJ20_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBK2384504.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(42147..42476)
                     /locus_tag="LGJ20_000379"
     CDS             complement(42147..42476)
                     /locus_tag="LGJ20_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBK2384505.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(42481..42666)
                     /locus_tag="LGJ20_000380"
     CDS             complement(42481..42666)
                     /locus_tag="LGJ20_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBK2384506.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(42663..45302)
                     /locus_tag="LGJ20_000381"
     CDS             complement(42663..45302)
                     /locus_tag="LGJ20_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBK2384507.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLYVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            45510..46499
                     /gene="ldhA"
                     /locus_tag="LGJ20_000382"
     CDS             45510..46499
                     /gene="ldhA"
                     /locus_tag="LGJ20_000382"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBK2384508.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            46610..47032
                     /gene="hslJ"
                     /locus_tag="LGJ20_000383"
     CDS             46610..47032
                     /gene="hslJ"
                     /locus_tag="LGJ20_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBK2384509.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(47029..47295)
                     /locus_tag="LGJ20_000384"
     CDS             complement(47029..47295)
                     /locus_tag="LGJ20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBK2384510.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            47569..51093
                     /gene="nifJ"
                     /locus_tag="LGJ20_000385"
     CDS             47569..51093
                     /gene="nifJ"
                     /locus_tag="LGJ20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK2384511.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            51459..52592
                     /gene="ompN"
                     /locus_tag="LGJ20_000386"
     CDS             51459..52592
                     /gene="ompN"
                     /locus_tag="LGJ20_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBK2384512.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            52733..53167
                     /gene="uspF"
                     /locus_tag="LGJ20_000387"
     CDS             52733..53167
                     /gene="uspF"
                     /locus_tag="LGJ20_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBK2384513.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            53343..54278
                     /gene="ttcA"
                     /locus_tag="LGJ20_000388"
     CDS             53343..54278
                     /gene="ttcA"
                     /locus_tag="LGJ20_000388"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBK2384514.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(54407..55780)
                     /gene="dbpA"
                     /locus_tag="LGJ20_000389"
     CDS             complement(54407..55780)
                     /gene="dbpA"
                     /locus_tag="LGJ20_000389"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBK2384515.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(55810..55983)
                     /locus_tag="LGJ20_000390"
     CDS             complement(55810..55983)
                     /locus_tag="LGJ20_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017372341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384516.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(56258..57241)
                     /gene="zntB"
                     /locus_tag="LGJ20_000391"
     CDS             complement(56258..57241)
                     /gene="zntB"
                     /locus_tag="LGJ20_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBK2384517.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            57496..58728
                     /gene="dgcM"
                     /locus_tag="LGJ20_000392"
     CDS             57496..58728
                     /gene="dgcM"
                     /locus_tag="LGJ20_000392"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBK2384518.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(58749..59312)
                     /gene="smrA"
                     /locus_tag="LGJ20_000393"
     CDS             complement(58749..59312)
                     /gene="smrA"
                     /locus_tag="LGJ20_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBK2384519.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(59642..60550)
                     /locus_tag="LGJ20_000394"
     CDS             complement(59642..60550)
                     /locus_tag="LGJ20_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2384520.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            60726..62036
                     /gene="abgA"
                     /locus_tag="LGJ20_000395"
     CDS             60726..62036
                     /gene="abgA"
                     /locus_tag="LGJ20_000395"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBK2384521.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            62036..63481
                     /gene="abgB"
                     /locus_tag="LGJ20_000396"
     CDS             62036..63481
                     /gene="abgB"
                     /locus_tag="LGJ20_000396"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBK2384522.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            63518..65044
                     /gene="abgT"
                     /locus_tag="LGJ20_000397"
     CDS             63518..65044
                     /gene="abgT"
                     /locus_tag="LGJ20_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HBK2384523.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            65055..65570
                     /gene="ogt"
                     /locus_tag="LGJ20_000398"
     CDS             65055..65570
                     /gene="ogt"
                     /locus_tag="LGJ20_000398"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK2384524.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            65765..66517
                     /gene="fnr"
                     /locus_tag="LGJ20_000399"
     CDS             65765..66517
                     /gene="fnr"
                     /locus_tag="LGJ20_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBK2384525.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            66669..67619
                     /gene="uspE"
                     /locus_tag="LGJ20_000400"
     CDS             66669..67619
                     /gene="uspE"
                     /locus_tag="LGJ20_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBK2384526.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(67669..67926)
                     /locus_tag="LGJ20_000401"
     CDS             complement(67669..67926)
                     /locus_tag="LGJ20_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBK2384527.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            68170..69201
                     /gene="ynaI"
                     /locus_tag="LGJ20_000402"
     CDS             68170..69201
                     /gene="ynaI"
                     /locus_tag="LGJ20_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBK2384528.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(69252..70865)
                     /gene="mppA"
                     /locus_tag="LGJ20_000403"
     CDS             complement(69252..70865)
                     /gene="mppA"
                     /locus_tag="LGJ20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBK2384529.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(71202..72101)
                     /gene="pgrR"
                     /locus_tag="LGJ20_000404"
     CDS             complement(71202..72101)
                     /gene="pgrR"
                     /locus_tag="LGJ20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBK2384530.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            72239..73159
                     /locus_tag="LGJ20_000405"
     CDS             72239..73159
                     /locus_tag="LGJ20_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2384531.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            73230..73367
                     /locus_tag="LGJ20_000406"
                     /pseudo
     CDS             73230..73367
                     /locus_tag="LGJ20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            73494..74222
                     /gene="mpaA"
                     /locus_tag="LGJ20_000407"
     CDS             73494..74222
                     /gene="mpaA"
                     /locus_tag="LGJ20_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBK2384532.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETLALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(74197..75162)
                     /gene="ycjG"
                     /locus_tag="LGJ20_000408"
     CDS             complement(74197..75162)
                     /gene="ycjG"
                     /locus_tag="LGJ20_000408"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBK2384533.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEAHVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            75281..75787
                     /gene="tpx"
                     /locus_tag="LGJ20_000409"
     CDS             75281..75787
                     /gene="tpx"
                     /locus_tag="LGJ20_000409"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBK2384534.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAVSVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(75831..77372)
                     /gene="tyrR"
                     /locus_tag="LGJ20_000410"
     CDS             complement(75831..77372)
                     /gene="tyrR"
                     /locus_tag="LGJ20_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBK2384535.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(77520..78581)
                     /locus_tag="LGJ20_000411"
     CDS             complement(77520..78581)
                     /locus_tag="LGJ20_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBK2384536.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(78578..79975)
                     /locus_tag="LGJ20_000412"
     CDS             complement(78578..79975)
                     /locus_tag="LGJ20_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBK2384537.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            80130..81128
                     /locus_tag="LGJ20_000413"
     CDS             80130..81128
                     /locus_tag="LGJ20_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK2384538.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(81239..82144)
                     /gene="ompG"
                     /locus_tag="LGJ20_000414"
     CDS             complement(81239..82144)
                     /gene="ompG"
                     /locus_tag="LGJ20_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBK2384539.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(82189..83271)
                     /locus_tag="LGJ20_000415"
     CDS             complement(82189..83271)
                     /locus_tag="LGJ20_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2384540.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(83285..83944)
                     /gene="pgmB"
                     /locus_tag="LGJ20_000416"
     CDS             complement(83285..83944)
                     /gene="pgmB"
                     /locus_tag="LGJ20_000416"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK2384541.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(83941..86208)
                     /gene="ycjT"
                     /locus_tag="LGJ20_000417"
     CDS             complement(83941..86208)
                     /gene="ycjT"
                     /locus_tag="LGJ20_000417"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBK2384542.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(86205..87260)
                     /locus_tag="LGJ20_000418"
     CDS             complement(86205..87260)
                     /locus_tag="LGJ20_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK2384543.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(87270..88058)
                     /locus_tag="LGJ20_000419"
     CDS             complement(87270..88058)
                     /locus_tag="LGJ20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK2384544.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTTCGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEERIRAEDPAQAYRDSLAWLRTC"
     gene            complement(88077..89129)
                     /locus_tag="LGJ20_000420"
     CDS             complement(88077..89129)
                     /locus_tag="LGJ20_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK2384545.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(89160..90002)
                     /locus_tag="LGJ20_000421"
     CDS             complement(89160..90002)
                     /locus_tag="LGJ20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK2384546.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(89989..90870)
                     /locus_tag="LGJ20_000422"
     CDS             complement(89989..90870)
                     /locus_tag="LGJ20_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK2384547.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(90891..92183)
                     /locus_tag="LGJ20_000423"
     CDS             complement(90891..92183)
                     /locus_tag="LGJ20_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2384548.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(92197..93876)
                     /gene="ycjM"
                     /locus_tag="LGJ20_000424"
     CDS             complement(92197..93876)
                     /gene="ycjM"
                     /locus_tag="LGJ20_000424"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBK2384549.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(94088..94402)
                     /gene="pspE"
                     /locus_tag="LGJ20_000425"
     CDS             complement(94088..94402)
                     /gene="pspE"
                     /locus_tag="LGJ20_000425"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBK2384550.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(94477..94698)
                     /gene="pspD"
                     /locus_tag="LGJ20_000426"
     CDS             complement(94477..94698)
                     /gene="pspD"
                     /locus_tag="LGJ20_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBK2384551.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(94707..95066)
                     /gene="pspC"
                     /locus_tag="LGJ20_000427"
     CDS             complement(94707..95066)
                     /gene="pspC"
                     /locus_tag="LGJ20_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBK2384552.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(95066..95290)
                     /gene="pspB"
                     /locus_tag="LGJ20_000428"
     CDS             complement(95066..95290)
                     /gene="pspB"
                     /locus_tag="LGJ20_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBK2384553.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(95344..96012)
                     /gene="pspA"
                     /locus_tag="LGJ20_000429"
     CDS             complement(95344..96012)
                     /gene="pspA"
                     /locus_tag="LGJ20_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBK2384554.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            96179..97156
                     /gene="pspF"
                     /locus_tag="LGJ20_000430"
     CDS             96179..97156
                     /gene="pspF"
                     /locus_tag="LGJ20_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBK2384555.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERAKETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDTIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(97276..98541)
                     /gene="puuE"
                     /locus_tag="LGJ20_000431"
     CDS             complement(97276..98541)
                     /gene="puuE"
                     /locus_tag="LGJ20_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBK2384556.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(98579..99859)
                     /gene="puuB"
                     /locus_tag="LGJ20_000432"
     CDS             complement(98579..99859)
                     /gene="puuB"
                     /locus_tag="LGJ20_000432"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBK2384557.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(99861..101348)
                     /gene="puuC"
                     /locus_tag="LGJ20_000433"
     CDS             complement(99861..101348)
                     /gene="puuC"
                     /locus_tag="LGJ20_000433"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBK2384558.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(101623..102180)
                     /gene="puuR"
                     /locus_tag="LGJ20_000434"
     CDS             complement(101623..102180)
                     /gene="puuR"
                     /locus_tag="LGJ20_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBK2384559.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(102207..102971)
                     /gene="puuD"
                     /locus_tag="LGJ20_000435"
     CDS             complement(102207..102971)
                     /gene="puuD"
                     /locus_tag="LGJ20_000435"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBK2384560.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            103183..104601
                     /gene="puuA"
                     /locus_tag="LGJ20_000436"
     CDS             103183..104601
                     /gene="puuA"
                     /locus_tag="LGJ20_000436"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBK2384561.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            104750..104914
                     /locus_tag="LGJ20_000437"
     CDS             104750..104914
                     /locus_tag="LGJ20_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384562.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            104904..106289
                     /gene="puuP"
                     /locus_tag="LGJ20_000438"
     CDS             104904..106289
                     /gene="puuP"
                     /locus_tag="LGJ20_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBK2384563.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            106423..106668
                     /locus_tag="LGJ20_000439"
     CDS             106423..106668
                     /locus_tag="LGJ20_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBK2384564.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            106981..108624
                     /gene="sapA"
                     /locus_tag="LGJ20_000440"
     CDS             106981..108624
                     /gene="sapA"
                     /locus_tag="LGJ20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBK2384565.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            108621..109586
                     /gene="sapB"
                     /locus_tag="LGJ20_000441"
     CDS             108621..109586
                     /gene="sapB"
                     /locus_tag="LGJ20_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBK2384566.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            109573..110463
                     /gene="sapC"
                     /locus_tag="LGJ20_000442"
     CDS             109573..110463
                     /gene="sapC"
                     /locus_tag="LGJ20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBK2384567.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            110463..111455
                     /gene="sapD"
                     /locus_tag="LGJ20_000443"
     CDS             110463..111455
                     /gene="sapD"
                     /locus_tag="LGJ20_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBK2384568.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            111457..112263
                     /gene="sapF"
                     /locus_tag="LGJ20_000444"
     CDS             111457..112263
                     /gene="sapF"
                     /locus_tag="LGJ20_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBK2384569.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            112331..112684
                     /locus_tag="LGJ20_000445"
     CDS             112331..112684
                     /locus_tag="LGJ20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBK2384570.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            113054..113842
                     /gene="fabI"
                     /locus_tag="LGJ20_000446"
     CDS             113054..113842
                     /gene="fabI"
                     /locus_tag="LGJ20_000446"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK2384571.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            113987..115114
                     /locus_tag="LGJ20_000447"
     CDS             113987..115114
                     /locus_tag="LGJ20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBK2384572.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            115182..117116
                     /gene="rnb"
                     /locus_tag="LGJ20_000448"
     CDS             115182..117116
                     /gene="rnb"
                     /locus_tag="LGJ20_000448"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBK2384573.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(117194..117298)
                     /locus_tag="LGJ20_000449"
     CDS             complement(117194..117298)
                     /locus_tag="LGJ20_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384574.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            117352..119337
                     /gene="pdeR"
                     /locus_tag="LGJ20_000450"
     CDS             117352..119337
                     /gene="pdeR"
                     /locus_tag="LGJ20_000450"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBK2384575.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            119484..119657
                     /locus_tag="LGJ20_000451"
     CDS             119484..119657
                     /locus_tag="LGJ20_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384576.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            119747..120496
                     /locus_tag="LGJ20_000452"
     CDS             119747..120496
                     /locus_tag="LGJ20_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2384577.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            120765..120983
                     /gene="osmB"
                     /locus_tag="LGJ20_000453"
     CDS             120765..120983
                     /gene="osmB"
                     /locus_tag="LGJ20_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBK2384578.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(121109..121435)
                     /gene="yciH"
                     /locus_tag="LGJ20_000454"
     CDS             complement(121109..121435)
                     /gene="yciH"
                     /locus_tag="LGJ20_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBK2384579.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(121435..122172)
                     /gene="pyrF"
                     /locus_tag="LGJ20_000455"
     CDS             complement(121435..122172)
                     /gene="pyrF"
                     /locus_tag="LGJ20_000455"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBK2384580.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIEPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(122365..123534)
                     /gene="lapB"
                     /locus_tag="LGJ20_000456"
     CDS             complement(122365..123534)
                     /gene="lapB"
                     /locus_tag="LGJ20_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBK2384581.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(123541..123849)
                     /gene="lapA"
                     /locus_tag="LGJ20_000457"
     CDS             complement(123541..123849)
                     /gene="lapA"
                     /locus_tag="LGJ20_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBK2384582.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(123998..124762)
                     /gene="pgpB"
                     /locus_tag="LGJ20_000458"
     CDS             complement(123998..124762)
                     /gene="pgpB"
                     /locus_tag="LGJ20_000458"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBK2384583.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            124932..125522
                     /gene="ribA"
                     /locus_tag="LGJ20_000459"
     CDS             124932..125522
                     /gene="ribA"
                     /locus_tag="LGJ20_000459"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBK2384584.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(125586..>127366)
                     /gene="acnA"
                     /locus_tag="LGJ20_000460"
     CDS             complement(125586..>127366)
                     /gene="acnA"
                     /locus_tag="LGJ20_000460"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBK2384585.1"
                     /translation="RATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKY
                     AKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEV
                     NATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGL
                     KRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIET
                     AIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGH
                     DRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYGW
                     QEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGR
                     YLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVS
                     IYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLI
                     GMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRC
                     RIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        31356 a        33438 c        31021 g        31551 t
ORIGIN      
        1 gcatcgttgt atttgaacaa ctccgggcga aagtgaattt cacgcgccag atgtgttgct
       61 ggatctacag aaaacatacg aacgcggcga cggaatccca ttcccatatg gaagaactga
      121 gcgtccagtt gacggttctc aacgttatgc cagagcgatt tttcggcgtc gtattgaata
      181 ctgttataag cctgcggcgt cattgtcgcc agcgtgtcag gtaacggacg aggcgcacca
      241 cgccacgctg tttgcgctaa gtcgtgcgcc attgactgta gaatggagaa gtcaaaacgc
      301 tgggtttgcc cgtcggcaat atcagaatct gccgcgaatg ccgcctgaga aaaaagagaa
      361 gcaatgccgc tggtaccgca cacggcggcc atagccattg aacctttaat aaatcgtcta
      421 cgatccatac ctgaaagtga gtccttctgg tgttgtgaat ggtcccgcgc tggtcgtttt
      481 cagcggcaag aaagtgctta tttatgaccg ctcactctag acaatattta ttaataatcc
      541 aattgttgaa ctgagagaat atgcataaat cggcattttt tttatgtcga tgggatgagc
      601 ctgattcgtt tgtaaaatca ggcaaatttt tgcaaaggcg gatggtcaga atttaaagca
      661 aaccacagcg acgtttactg cgttcttccg cttcctggta aagctggaac tcatccaaaa
      721 ccggacaacc caacgcctgc tcaaatgctt cgcgactggc attaccgtga ctgcgtgtct
      781 gcgtttcatt acccagattt gactggaaga tccccgccgc gctaacgggc aagaaatctt
      841 cataggtaat gggttgcgcc gcgacccaac cacgttcaat taagggctgt ggatcgtctc
      901 cgggatgaat cgcctgacga tgcgcctcac ctgaaggcgt cagacggtac cggaaccatg
      961 ccagcccttg ctgacgcatt aaaaactcac tgtcaggaaa agtgcggaag gtttcctgta
     1021 aatgcatttg gtgagtgaga ttatcctgcc cggttccagc gttacgcaga agatcatcat
     1081 acagttgtcg ccctttcggc gttaatgcca cgccacgctg ctcaatttca ccaaagcgcg
     1141 cggtatgcgt gccctgtttc tgccccgcaa acaacaccgt ctcttccagt gctttaaagc
     1201 tggtctggcg tagtaaaatc ggtacctcgc ggcgcggcgg cccctcgatg agaattttgg
     1261 gttcaattcc gcattcaggc atcatcgact gcacccggtc aatatccagc gtacgtggtg
     1321 tcaggtggtt gatatggcat ccaggaaaac agaccacatc agcaatcaac cgatgttcgt
     1381 tgtgcaaggc gcgataggtt tcttcatcta ccgttgctga ctggtgccag cgaaacgttt
     1441 ccagggcttc ctgcacaaac tcctgtgcct gtgtttcgtt aaaaccgccc tgctgctcat
     1501 attcctctaa cagttgtcga caacgtgggg tgaagatatc gcgctgacgt agaatctccg
     1561 ccgctttctg gcgcaaaatt tcgttctcga taagctcaag gcggagtaag gaggtaaaaa
     1621 cgcggaaggg attacgcgcc agagaagcat catcaatggg ccgaaatgct gtcgaatgta
     1681 ccggcacccc tgcctgcgag agatcgtaat agctcaccgg gtacatcccc ataatggcaa
     1741 acatccgccg aagagtagca agctcttgtg cagtcccaac gcgaatcgcc ccatgacgtt
     1801 caacattcag tcgcgccagc tcgtctgcat ttaccatttt ttcgtgcagt tgaggattgt
     1861 tttccagcac agccagattc acatcagcta ccagttccag cagcgtgcca tattgcggaa
     1921 cttcttgctg gtacatggct gacattgcct gcgaaaactg ttcccgaatc tcatccgccg
     1981 tgatgctgtt cgccatagtg tctgcctcca gtggtcagta atctggagtg taggtaaccg
     2041 cattcactct tgcgggaaga atttacaaac tgtgatctcg ccgcgaaaac atcaatatta
     2101 tccattttgc tgtaacataa ttgctttaat tgttaataat attttgcaat caagttatca
     2161 taatcaaaca acttcacttg tcagcgacac cgcttcgttt ttaacatcgc ttatggaaaa
     2221 aaatagtctg tttagtcagc gcatccgttt gcgccacctt catacattcg tagctgtcgc
     2281 acaacaagga actttggggc gcgcggctga aacccttaat ttgagtcaac ctgcgctctc
     2341 taagacattg aatgaactgg agcagctgac tggcgctcgc ttgtttgagc gtggtcgtca
     2401 gggggcgcaa cttaccttac ccggcgaaca atttttaacg catgcagtca gagttcttga
     2461 cgccatcaac actgccggac agtcgcttca tcgtaaagaa ggtcttaata atgatgtcgt
     2521 cagggttggt gcactaccta ctgcggcact ggggatatta ccttcggtta taggtcagtt
     2581 tcatcagcaa caaaaagaga cgaccttgca agttgcgaca atgagtaacc ctatgattct
     2641 ggcgggttta aaaaccgggg aaatcgatat cggcattggt cggatgtcag atcctgaact
     2701 gatgaccggg cttaattacg aactgctgtt tcttgaatcg ttgaagctgg ttgtccgccc
     2761 taatcacccg ctacttcagg agaacgtaac gctaagccgg gtgctggaat ggccggtcgt
     2821 tgtatcacca gaaggcactg cgccacgcca gcattcagat gcattagtgc agagccaggg
     2881 ctgtaaaatt ccttcgggtt gtatcgaaac gctgtctgct tcgctatctc gtcaacttac
     2941 ggttgaatac gattacgtgt ggtttgtccc ttctggcgct gtaaaagacg acctgcgtca
     3001 tgccacgctg gtggccctgc ctgttccggg acatggtgca ggcgaaccga ttggaatact
     3061 gacccgcgta gatgcgacgt tctcttctgg ttgccagttg atgattaacg ctattcgaaa
     3121 atcaatgccg ttctgaaagg tgaagggatc tgtcgatccc tccttgaaca ttttcacacc
     3181 gtagcgaaac taactggttc acccgctccg cgaggttctg ccgacacaga atgtttgtgc
     3241 agacggaata catccaccgc ctcagttaat cgtgccgcct gttcttcaag ggacaccgcc
     3301 gccgctgagg cctcttctac cagagaagca ttctgttgcg tcaccttatc catttcagaa
     3361 atcgcctggc taacctgcgt tatgcctcta ctttgttcat ccgaggcggc ggcgatttcc
     3421 tgcatgatat gtgtgacact cgcgacggca tcaacaatag tgctcatggt tttccctgcc
     3481 gttgccacct catccgaccc caggtcaatt aacctgactg attcactgat caagccttca
     3541 atctctttcg ccgcttgggc gctgcggctt gcgagtgtcc gtacttcgct ggcgacaacg
     3601 gcaaatccac ggccttgctc acccgctcgc gcggcttcaa cggcagcatt cagtgccaga
     3661 atattcgtct ggaaagcaat actgttgatg acggcggtga tctcggaaat tttcttcgaa
     3721 cttgtagaga tagcgcccat cgtttttact acaccggaaa ccgtctgccc gccatcgctg
     3781 gctttaatag aagcctcttg cgccagtttg ctggcatgat gcgcgttatc cgcattctgt
     3841 ttcaccgtcg cagtgagttg ctccatgcta gcggcagttt gttcgatagc cgccgcttgt
     3901 tcttcggtgc gagatgacag gtccgcattg ccagctgaaa tttcgctggt gccacgataa
     3961 atctcttccg caccctgtcg aacagtccct actgtcatcc ccagtgaatg ctgcatttgc
     4021 tgtaaatgac gatttaagcg accgatttca ttacgacccg ccggttcatc gttcatcgtc
     4081 agatcgccac tagcgatttt ttcaatccgt tgtgcggcat gttgcagtgg acgaatgacg
     4141 atccgacgta tcaccataaa tgttatcagc gtcatgacca gggcaagcac aaacgcgcca
     4201 atcatgaaca tcccacccag gcgggtgcgc tgatgggcca gttccgccag ttggttggct
     4261 ctggtgctac gtatcttaac ggctttgttc aaaatatcgg tataagcatt atccagcggt
     4321 cggatctgct cactttcatg attaataatc gcttcaaaca tgccattttt ggcatatttc
     4381 aacataggtt gcatacccgt gatataagcc tgaaagcgtt gatttaattc agtgtcgagg
     4441 gcttcatcag caggtgtttt caccggccga ttctgataag cacgataacc ttgctgcgac
     4501 tgtttaatct ccgattcggc ttgcgcaata tttcgcttca ttgcttccat ttccgcaata
     4561 cgactcgcag ccccggcctg aatcatattg atccgggctg aacgcaaaaa atctgaactg
     4621 ttcgctaacc cggtccgaat ctcaatctcc gcagtaacat cacgctgatc gcgatcggcc
     4681 tgccagagga aatagccagc cagagcagaa cttaatgcga atagaactaa gataccgcca
     4741 agaatgcagg caaataacgg aaccagtctg atgtgatgca aaaaacctaa tcgctgtgag
     4801 ggagttgtat tcattaccgt tgtctctcgt ccaggtttac tcctgataga aagtcatcgg
     4861 cacttgacgg taattactta tgaccaaaaa cctctttgcg tcacattttt acaacaatta
     4921 ttgcagagaa aattaatgct attggaatga agagagtaac gaatggctaa actggctaac
     4981 ccgaatgacg aaaaattctg gaaaacagca cgcaatttcg tgaagtgatt attttcgtcc
     5041 ctaaggaaac ctcataggat gcctgcgggt gcctgagact ttctgttttc aggctaaccg
     5101 ggaatcagaa agacgaaggc cccgaccgat atttctatca accgaggcca acgaatgaac
     5161 ctagcaattc agatcttagc ctcttacccg ccatccggca aggagaaagg ctatgaagca
     5221 caaccctctg gtggtgtgtc tgctcattat ctgcattacg attctgacat tcacactcct
     5281 gacccgacaa acgctctacg aactgcggtt ccgggacggt gataaggagg ttgctgcgct
     5341 catggcctgc acgtccaggt aagggcaagc gcggggattt tccccgcgca tctgactagc
     5401 agattcattt tctcatggca ccccttttac acttcagtta gtgcatgttt ttttcgaggc
     5461 actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg agatcccccc
     5521 tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa gcgttcccat
     5581 aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat cttatgttcg
     5641 atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag atcgggtgaa
     5701 ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat ttgtcataca
     5761 aataaatata ataggcgctt ccgatgtaga cccgtattct tcgcctgtac cacgggtcgg
     5821 ttttagtaca ggcgttttct tgaaatatcc ttcaggaacg tttacgcggc atcatgcgta
     5881 gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg tgcaacccga
     5941 tcacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta tcaacccgtt
     6001 cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac gggttgcccc
     6061 ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga taaatcacca
     6121 aatgtcccaa atgcgccagt cccgtcatca tcggttttgg cttaggtata atcggcgggg
     6181 ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt gagataccac
     6241 aggaaacatg aaccatgttg atgagtggcc gatcgctacg tgggaagaaa ccacgaaact
     6301 ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg ctgatttgta
     6361 accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc gttaacataa
     6421 cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg ttacgtttca
     6481 ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa ggatcaattt
     6541 tcatccgaat gttcctgaca ggagaaaacc atgagtaaag ttggtattaa cggttttggt
     6601 cgtatcggtc gactggtgtt gcgtcgatta cttgaagtca aaagcaacat agacgttgtc
     6661 gctattaatg atctcacttc cccaaaaatt ctcgcctacc tgctgaaaca tgattcaaac
     6721 tacggaccat tcccctggag cgttgatttt acggaagatt cacttatcgt tgatgggaaa
     6781 agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc gaaaggtgca
     6841 gaaatcattg tcgaatgtac tggcttttat acctccgccg agaaatcgca ggcgcatctt
     6901 gatgctggtg cgaagaaggt gttgatttcc gcccctgccg gtgaaatgaa aactatcgtt
     6961 tataacgtca atgacgacac tctggatggc aacgacacca ttgtttccgt ggcgtcatgc
     7021 acaactaact gtcttgcgcc gatggccaaa gccttgcatg acagtttcgg gatagaagtc
     7081 ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga tggcccacgt
     7141 ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca cactacgggg
     7201 gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa aggtcatgcg
     7261 caacgcgtgc cggtgaaaac aggttcggtc actgaactgg tatcgattct cggaaaaaaa
     7321 gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa cgagtcattt
     7381 ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt cggttcggtg
     7441 tttgatgcca cgcaaacgga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc
     7501 gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc
     7561 gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctcttttac tcattacgac
     7621 tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg
     7681 ccaataccgg atttacgcca tccggcgtgg aagccttgca tagcttcgaa gttttcacgg
     7741 ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt
     7801 tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcctct
     7861 tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc
     7921 gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct
     7981 accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc
     8041 tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca
     8101 gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca
     8161 taaatgcctt tctgtacata aatacgttct gcacagttac acacttgccc actattaatg
     8221 acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata
     8281 gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc cgcagtcgcc
     8341 atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta
     8401 cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc
     8461 ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattggttgg cgtaaattca
     8521 ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg
     8581 aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac
     8641 aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc
     8701 cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc
     8761 tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatc
     8821 ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct
     8881 ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt
     8941 atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc gtctccacgc
     9001 caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac
     9061 tcctgtgatt tatatgtttg ttttctgtga atgcattgtt aattaacatc aatcatcaga
     9121 gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga
     9181 ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac
     9241 gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt
     9301 cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg
     9361 cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag
     9421 tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc
     9481 tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg
     9541 attgtcccca gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg
     9601 aacaacgata gccgtatgaa ctcgttctac ggcctgtttc aatagcgcga tgctaaagcg
     9661 tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg
     9721 ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat
     9781 agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat
     9841 accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat
     9901 agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc
     9961 ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg
    10021 cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac
    10081 tgcgtgagat aatagtgaga agcggagcac tctgaaagga ggaagtcgta tgtccttaac
    10141 cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac attattcatc
    10201 ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc gttttagaat
    10261 taataaaaaa cccggcgcta aatgcttacc gggttttttc tttatcaggc aaatagcagg
    10321 gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc ggataaggcg
    10381 taccaagttg ttgagcgaag taactaacgc gcaactcttc tatcatccaa cggatctctt
    10441 tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct
    10501 gctggacgtt ttcgactttc agcatctgag cacggtcgcg atgtggatca accgccagtt
    10561 tttccagccg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc
    10621 cgttaccagt gacaaatccg cgatatacca acccgcccat ctgcgcttta atgtcagaaa
    10681 gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata ttgaacactg
    10741 ccgtaagaat ttgctcgacc tgcttcgcaa tatctaccac cgtgtcgttc agttcggcac
    10801 gcactttctc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca ccattggcgt
    10861 cgatcagttg atccacaccg caggagatac agtcgtcgat cagctccagc actttgccat
    10921 acgggttaaa gtacagtcca agcttggctt tgtttggtaa cttctcatgt aaatacttga
    10981 tcggcgaggg aatatttagc agcagtagac ggcgaagacc gttccacatt gcctgttttt
    11041 gctccagcgg gttatcaaac agtttgatcg ccacactgtc acgttcatcc accagcgccg
    11101 gccacgcctt cactttatag ttgccgcgct tctgttcgta gctttccggc agttgcccaa
    11161 agctccagat atgcagcccg ctctgctcga taccgtcatc cgccaccgcg gacagcgttt
    11221 cctgcacttt gcctttcagc gcatctttca gatcctgtaa tgagcgccct tcttttagct
    11281 tcttattttt gtcgtccacc acgcggaagg tgattttcag gtgatcgggc acctgatccc
    11341 agtgccagtc ttcgcggtca acggtaacac cggtcatccg ccgtaactcg cgctcaaggc
    11401 tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg
    11461 gtgcgggtac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga gcaatcacca
    11521 gttcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc tggttaagta
    11581 acggcagcgg aatatgtacg gtcacaccgt cagcatccgc gccgggctca aactgatagc
    11641 tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc ttgctgattt
    11701 tttctgcgcc ctctttgatc aacatgcttt tttcaaagtt gagcaaatca ggggtttcgc
    11761 ggctgacttt tttccaccag ctgtcgaagt ggcgtgcaga gatcacatcg tggctgatgc
    11821 gctggtcgta gaactcaaac aacgtttcgt catcaaccag aatatcgcgg cgacgtgatt
    11881 tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac
    11941 gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacgg cataacgccg
    12001 gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca taaacagtga
    12061 ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgaggttcg ctgtaggtgc
    12121 gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc gcagcaatgc
    12181 gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc ggcggttttt
    12241 tgaataaacc agaaccggga aagatggaga aacgcgcgtt acgtgcgccg gtatattctt
    12301 gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac gcaatgtgaa
    12361 tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc accacctggc
    12421 gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga taatcggtac
    12481 gacacaggcg acggaaggcg ttggaagaaa gcgccttttg ctgctcgcca agataattcc
    12541 acagattcac aaacgcgagg aagtcagact ctttgtcgtg gaagcgacga tgtttttcgt
    12601 ccgatgcctg ctgtttatcc atcggacgct cgcgcggatc ctgaatggaa agcgcggacg
    12661 tgataatcat cgcctcacgc acgcagccat gtttttgcgc ttccagcacc atacgcgcca
    12721 gacgtggatc gacaggcaac tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc
    12781 tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg ccatcctgga
    12841 tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc agccccagcg
    12901 cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga tcggtaaact
    12961 ccgggcgcga aaggaaatcg tcttcggaat agagacgaat acagatccct tcggacacac
    13021 gcccacagcg gcctttacgc tgattggcag acgcctgcga aatgggttca atcggcaaac
    13081 gctgcacttt ggtgcgatag ctgtagcggc tgatacgtgc tgtaccgggg tcgataacgt
    13141 atttaatccc cggtacggtc agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc
    13201 gtccgctgtg cgactggaac acgcggttct gttcgctgtt cgaaagccgc gcataaagcg
    13261 gcaagatttc ggtatgacgc agatttagct tgttcagcgc atcggcggta tcgcgaattt
    13321 cccgctcacc actcataaag atcagaatgt cgccagggct ttcctgactc agttcgtcta
    13381 cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc gtcggcttct tcaacaatcg
    13441 gtcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc ggcgcattat
    13501 taaagtggcg cgaaaagcgt tccgggtcga tagtcgcgga agtgataatg atttttaggt
    13561 caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata ttcaggctgc
    13621 gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg tcttgctgga
    13681 tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca ctaacgtgat
    13741 cgctgaaacg cactttgtaa ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa
    13801 tacggttcgc caccgttctt gccgccaggc gacgcggctg ggtatggccg atcagccctt
    13861 taaccccgcg ccccagctcc atacagattt tcggtaactg agtcgtttta ccagaacccg
    13921 tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaatg tcctgttttt
    13981 tctgactaac cggtaaattg tcaggataag taatttccgg tcgtgccgct tcacgcagca
    14041 ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg
    14101 catcaggatt tttaaccttc ttaacaccgt gcagacgccg agaaaaacgc agtctgtcgc
    14161 gcggcatcag cgaatccagc cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg
    14221 tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga tagatataga
    14281 gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc ttcgatttat
    14341 tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg gcatcgccca
    14401 ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc cagcatcctg
    14461 gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag
    14521 cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc ggtactggat
    14581 ggcgaactgg ttggtgcact gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa
    14641 gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt tatcgttatt
    14701 gcggcaccga tgtataactt caacatctca actcagttga aaaactattt tgacctggtt
    14761 gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct ggtaacgggt
    14821 aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc aacggacctg
    14881 gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt gaaatttgtc
    14941 ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa
    15001 gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg gactgtagtt
    15061 cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc tttgtgatct
    15121 tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc atcaacggtt
    15181 ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg cgataatccc
    15241 aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg
    15301 gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac acagaaatag
    15361 agtcgatctt ttgtcgctcg tccgcgaggg aattcaaagg tgacgtccag aaccgcattc
    15421 tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtcat tttgctcacc
    15481 ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg gcagggcaat
    15541 gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt tgttatcgcg
    15601 ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca acataaccag
    15661 actgaccacc ataactgaat catcaccatt agctccatca acacaatgca cgagcccgcg
    15721 cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa
    15781 cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca
    15841 tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa aaaccatccg
    15901 ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca aagtagccag
    15961 cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc
    16021 gaaaatagcc atcccgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga
    16081 tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg
    16141 cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag atccagactc
    16201 cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga
    16261 ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag agatccatag
    16321 gtgaagaaga aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc
    16381 cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc actcatcaat
    16441 ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct ccccttgcga
    16501 acgcacgcgc atcacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag
    16561 ctccagttgt gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc
    16621 attggcaaga cctgcgaggg agttttttac ctgctcattt tcgggaaaaa gctcatacag
    16681 gccagagaca atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa
    16741 cgcatcgccc tgttcaaaac gcacctgccc agacattccc cgttgggcaa tcatctcttg
    16801 cccctgtgca acatttaact cactgtaatc acgtaacaaa atatcgcata cggcaggttc
    16861 attctccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggat
    16921 ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagcattt gtaaatgggt
    16981 tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt cgactaaacg
    17041 cccgaatgcg ttactacctt gtggttgatt ttgatagaca taatccagcg aactgcctga
    17101 atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgccccga acaatttcat
    17161 cgccatacgc ataaagcgat acgccaaatc aaccggcgat aatggcacgg gtccaccaga
    17221 aagtagccgc catcgatccg ctgatggtcc tgtgcagtct tcatgctgat aatcaaattt
    17281 ttgcgatttg ttagcatata aatgactaat aaagctttgc attttttcaa atgccagcgc
    17341 cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttgag
    17401 agggctacgt aaacgctgat aaaaatcgat ctgcggttgg cgatgcacca catagtcatc
    17461 gcctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc
    17521 agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc
    17581 attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga
    17641 attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg
    17701 cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa ttgcaggcgc
    17761 ataatcatga acccatgtgg caaccagcac tgcgccgacg ctttgcgcga tcacaaccac
    17821 ctcttccagt ccaacctggc tgtcgctggc agcaaaacgg acaaattcat cgacatcctg
    17881 cactgaacgc gcaagagatg ggctataacc acgcggcccc gaagtttttc catgcccccg
    17941 ggcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga gatgttgtag
    18001 acgaccagaa tgttcatgac cccgatgaaa taagacgatg acctttttcg ccccgggctg
    18061 taaagcgggc cagtgccgat aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc
    18121 cccagggatg cgtgaatttt ccattttctc tattttcctt cagtaacagc tgtagcgaat
    18181 aaaataaaaa aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc
    18241 atgtcctggt aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat
    18301 cacgacatcg ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa
    18361 tgtatttagc ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgatgac
    18421 gccccccacc agaccctcca gcgtttttcc cgggctgact ttggggacca cttttcttct
    18481 gccgcaggat tttccccata aatactgtgc aatatcgttt gactcggtta atgccaaaag
    18541 aaataacacc agtaacgccc cgccctggat attcatggat ggaaacatta ataaccaggc
    18601 ggcatgactc aaggcgaaaa cggtcattat ccagccccaa aaaatagcgc tcacggtatg
    18661 cagaaaaccg gagggttcac ccacaaaaac tcgccaggtg gctgatatca gaaacccaga
    18721 caaaggtata aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag
    18781 aggaataacc caatataacc accgcggaaa atgtatggat ataagcgtac aatattcttt
    18841 caatgccaga aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg gacctgaaat
    18901 caccagtgaa aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacctcacc
    18961 cccttttctc tccggtaacc gccatagcag aaaacggttt atcagcgttg ctaaaattaa
    19021 caacgtgaaa agtgttgcca gagatttttc cagcattatc tttcagcact cataaaaagc
    19081 acactccgac aacgattaat cgcagtccag agaagcagaa ttgaagcaat gctccataaa
    19141 agattattcc attgcatcca ctgtggataa atggaaacgg ccagccccca caacccaaat
    19201 attaacgcac gatcgctttt gccgaatgga ccggcataac tgcgaatacc gttaattgtc
    19261 tgcgcgagta aaccacaaaa ctcagtcaat atggtgcaaa ataacatgag tattacgagt
    19321 aatgcgttac tttccggtaa aaataaaaac ggtaaataga gcgcaatatc ggaaataaca
    19381 tcgccagttt cattcaaaat cgcccccaga cgtgtttgct gattgcactc acgcgccaac
    19441 atgccatcca gcgcattgag cgccatacgg ataaaaagca cgatgggcaa tagcaaaaag
    19501 aggatgggtt gtgccaccaa caccaacagc aatccggtaa gaagagaaag cgccagtgca
    19561 gcaagagtga tgtgattcgc tgtaacgtgg tgcttgtaaa gccaaaacat cgtcggcctt
    19621 aacagcgact gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc
    19681 ggtttgcgct atggtgcatc aattcccctg acggagatat cgcaacacgc cgcttcatta
    19741 aaaattcaga atatatcaag gtattgggga ctcactgtca ggaataatta tccccaatta
    19801 atcaaagggt taagaaatat tacgttgtgg atcaatacaa ctcatcaagt tttctcatcg
    19861 cttgttcccg tattgcgcgc gtcttaccgt taagcgccag ctggcggact aaatccagtt
    19921 cagggttaac aagctctaac gccagcaaag gatcacgttt cagcctcgcc tttaatacat
    19981 caactggaaa acggggatta ttcatcgcca caatccacca ctcgggatca atttctgccg
    20041 ccagtactgc aggcggtgtg tggcggttgt taagtgcgcc gaacttgaaa aatgtgagat
    20101 tgcttttcgc cagtgtactc caccatatac gatccggttg ttttgccagt acatccagtt
    20161 gtgctaacgc atcggggtcg agtgcataat agacgcgtaa tgcttcatca acctgcgcac
    20221 gggagctgtt ttcctgcctc agaaaaaact tcgcccaaat atcattgcgt ggcgtatttt
    20281 gctgtaattc cttgaggaca acagcgggta aattatccca actaactaat gcactctcta
    20341 aaaagtccca gtcttccaaa cgtatcaggg cggtggcatt taatagcatc tggtcatcaa
    20401 cttcaccgtg gctgataagc tcagacaaaa tcatatcagc gctggtttca tacgcagctg
    20461 aatctgcttt cggattattt ttccgatgcg ataaagcacg ttgccaaatt ttttgtttca
    20521 tcgacgtgct cagatactga tttttcgcca gattactcca cccctcagga tcgttgaaac
    20581 gtgtcaataa ccgttgtaaa gcagcgtcag agtgaattac ctgcatatcc cgacagcaaa
    20641 agtaggcagg ggattttttc tccatccttt taacaaaatt aaactgtaag ttatctggca
    20701 atgctaaaaa taacacacgt attagatcat cgtcattctc gaatttttgg tataaatagg
    20761 ctgcaacttt gtcactctct tgtggcgtca tccgcaataa ttgaccatgg tgtttcatct
    20821 ctgccagctt gaacgttaag cttgtgacaa cattgtttga tgaatcgttc gccaactgca
    20881 ttgcctgatt gtaggtgagt tgtcctccag caccgacctg acgaatagag gaatcatcac
    20941 tgcggatata gccatcaata atggattcag gcaataacca cgcatcttta taaagctcct
    21001 gaatgatcat atcccaatct cttttcgcca gagtcattac gatatccaga gggagatttt
    21061 cacgtagcgc aagattcaac ggaccgacac catcaggtag acgactcatt cgcgtcatta
    21121 tttcgacaga gagcggttca cggtcgagca accccagaac aacaggctgg taacccaatt
    21181 gctgatcggt caactccaga atctcattgt caggcatata tctcgccata ctttcaatcg
    21241 tttgttcatc attgctatta ataagttgtt tgcgtaggtt agctgaaatt cctttaagcc
    21301 ttgggagagt tcgcattatg gcaagatcaa atgtcgcaat atctcgtcct tcaaatgcag
    21361 caacgttgcc ttcatccagc atttgcaatt cctgcactaa ctcaggagaa ggcttagggc
    21421 gaaacagcgc ttcttttttc atctgattac agacattccc tgccatgcga atattgcttc
    21481 gcttaaaaat ttctttcaga gcatcttcat catttattag accccaataa tatgcatcag
    21541 gatttattat catcagcgct tttttgacat tttcagtgtc atttttgtca ctcgcacgga
    21601 ccatgaattc actatttgtc gaagacgcaa caacaggtgc aattatgtca ttgataaata
    21661 tcccacgcgc attaataccc ccttaatcgt agacggatta tcgtaatatg agtcgataaa
    21721 taccagtcca cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt
    21781 cgttgccgtc acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt
    21841 acctttgact gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc
    21901 taccactaaa tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag
    21961 attcacgcta ctgttgatag cttgttcaac ttgttgttta tcttcagcgg tagctgcaga
    22021 aaaaaatggt tcaatatcaa ctgcatttgc aacacatcga agttccgcct gtactggaag
    22081 actcagcata aacaggggaa gtaaacctat ccagctgcaa atcctgggct taaacatatg
    22141 cactccatgc cattaaaaaa gaaacaaaag ggaaatgcat ctttcccttt tgtatattcg
    22201 ttattctcgc gaaataccat ccaacgtaga caacacttcc tcagaaagat gcaatttttc
    22261 agcagccata ttctcccgta aatgcgcaac agaagacgtc cctgggatca gcaaaatatt
    22321 cggtgaacgt tgtaacagcc acgccagcgc cacctgcatg ggtgttgcac ccaggctcgc
    22381 agcaacatcc gacagcgtag aggattgcag cggtgtaaag cccccgagcg ggaagaacgg
    22441 cacgtaggca atgccatcgt gggccaaagc atcaatcatt gcatcatcag cacggtgcgc
    22501 gatgttgtat tcgttttgca cacagacaat ttcggcaatc ttgcgcgcct ctgcaacctg
    22561 cgtcggtgtg acgttgctca ggccaatatg ttttaccagg ccttgttgtt gcatctctgc
    22621 cagcacggtc aggctggcct caatcgatcc ttccgctggg ccatgaccat ctcccatcat
    22681 aacgcgcagg ttaaccacat ccagtacgtc caggccgaga ttacgtagat tatcgtgcac
    22741 cgctttttgc aactctgccg gagaaaaggc gggcaaccag gaagcgtcct ctccacgccg
    22801 cgcaccaatt ttagtgacaa ttgtcaggtc gtcagagtaa ggataaagcg cttcgcggat
    22861 aatctgattg gtgacgtgcg gaccataaaa gtcgctggta tcaatgtgat tgacgcccaa
    22921 tgccagcgcc tcgcgcagca cagttatagc gacgtggcga tctcttgggg gaccaaaaac
    22981 cccaggacct gccagttgca tcgcgccata accaagacgg ttaacggatt ttgtaccgag
    23041 agtaaatgta ttgctgctca tcgcaccctg ccctgtaata aaacggatta agaaaaatca
    23101 gtatagaaac gaaaagagat aaataatgcg agggcgtaaa caagattact ggatgagttg
    23161 ctctattgtc ctgaaaaaca caaaacaaaa cgcgtaaagc gccgcatccg gcaataaaaa
    23221 aaccgcaacc tgtatcagcg ttgcggttta ggtatcacat gctaaaaatt agaatgttca
    23281 gtatctggac gttactgttt cggtcgcaga ttctcatcaa ataccagacg tttcctgttc
    23341 ggttcaactt cccgcaatgg ctcaacttga tgcaacgtct gcttacaacg agtcaccagc
    23401 acctgatact catgcgtacc ctgctttttc catgccagct cctgctcact gagttcacga
    23461 atcgctttcg ccgggctgcc gacaatcagg taattggcag gcatttctgc tttggctttc
    23521 acaaatgcgg atgcgccaac aatgctgttc tcgccaatca ccgcgccgtc catcactacc
    23581 gcgttcattc ccactaatgc attgcggcgg ataatgcaac catgaaggat agcgctatga
    23641 ccaatatgtc catcttcttc cacgacagta tcctgctcgg gaaaaccgtg cataacgcaa
    23701 ttatcctgaa tgttcgcgcc atctttcacc acgatacgac caaaatcgcc acgcaggctg
    23761 gcatttggcc caacgtaaac gcccttgcct agaataacat cgccgatcaa taccgctgtc
    23821 ggatggacaa aactctcttc tggcacaacc ggagtcagac catctatctg gtaaattggc
    23881 atatcgcctc ctgttcaata ttcaaaccgc caaaacgttg aaaatacagg cttcccggcg
    23941 caggcagttc accgaccgag gtttcacctt tttcactaac gaacgctaaa gcagcaggcg
    24001 ctacgcgctg ataaatgttg atacacagct gacgcgccgt atgccctgcc cagtgcgccg
    24061 gaagcaactc ctctggcaac aatgggtctt taaggacgac acggcgataa aaatggatca
    24121 gtaaaagctg aatatgaaat gcacgctccg gggttaactc gtctgctgcc tcttttaaca
    24181 gcggcaccag cgggcggaat gactgaataa aggtttcgta catggcattt tgttcagtta
    24241 aatgccagca ctcttccact ctggcacgca gtgctgcacg agaaagcgcc agtggtattt
    24301 gcgcttcaaa acaaatcacg ttatccgcca cccccgcttc atgcaaaagt gtctgtacat
    24361 ccgccagttt ttgcgacggc gatgccatca ggctgggtgc cagtgcgcca aaaccttgcc
    24421 agatcaactg ctttttgaca tcagccagcg ttgatttatc taaaccttcc gagagcaaca
    24481 ggagccattt accatcccat gcaggttgct ctgcgcgata aattttactt tctgcccgtc
    24541 gcgtcaggcg caagccttta tcactgaggc tatagaaact gcgtcgcccg atgcgggaaa
    24601 catccagcca gccttcttta ttaagacgaa acaaagcggt gcgcacgaaa cgctcaccaa
    24661 atcccagccc ttccagcaaa gcagccagac taccaagcca gatttcacca ccacgatggg
    24721 aaagcgaatc accatacaga gaggagatca aagatgtgcc actgaccgga acagcgttta
    24781 cagcatgttg gataaaagta tcaagtttac tcattcgctt cagtctgttg ttattgttgt
    24841 gatttatgaa tcatagcaca ggtcgtatcg acttctggcc ctggcgcttt acctcaccag
    24901 ggccagacga cacgtcaggc accaacaata ttgcgcagat caaacacccg acacgccttg
    24961 ccttctgaac gcgggatact gccacagtta acgatcatca catcggtaga gatccccacc
    25021 atcgacttaa tccgatggcg cagctggtgg cacacctggc aacgctgctc atgcgtcaat
    25081 gtcaggctgc tttctttcaa ctccactttc acagaaagtg aatcaagatg cccccggcgg
    25141 ttcacctcca gttggtaatg gggcgataaa tgttcgaatt tgacaatctc ttcttccagt
    25201 tgtgacggga agacatttac accgcgaatg atcaacatgt catcgctgcg tccgctgatg
    25261 cgatccatgc ggcgcatagt ccgcgccgtt cctggtaaca gtcgggttaa atcacgcgtg
    25321 cggtaacgaa tgaccggcaa tgcttctttg gtcagcgtgg tgaataacag ttcgccatgt
    25381 tcaccatcgg caagcggtgt gccgtcatga ggattaacaa tttcaggata gaaatgatct
    25441 tcccaaatgg ttgggccgtc ggtagtttcc agacactcca tcgccacccc cggccccatc
    25501 acttctgaca ggccataaat atccagtgcg gtgatcccca gacgccgttc aatctcttta
    25561 cgcatggcct gtgtccacgg ctcagcacca aacactccaa cccgcagcga gcaaccgctg
    25621 gcatcaccgc ccaactgccg ctccagctct tcaatcaggt taaggcaata agatggcgta
    25681 accatgatca tatctggttg aaaatcgcgg atcagttgcg cttgtttttc cgtctgccca
    25741 ccagacatcg ggatcaccgt agcgcccaga cgttcggcac catagtgcgc gcccagccca
    25801 ccagtaaaca ggccgtaacc ataggcaaca tgaattttgt ctttgggcgt gccacctgcc
    25861 gcacgcaagg aacgcgctac aatattggcc cagttatcaa tatcgttttg cgtatagccg
    25921 acaactgtcg gtttacctgt ggttcccgaa gaagcatgaa tgcgcaccac ttgttccatc
    25981 ggcacggcaa aggtgtcgaa gggatagttg tcgcgcagat cctgtttggt ggtacaggga
    26041 aatttacgca gatctgaaag ttccctgaaa tcatcaggat gtacgcctgc tgcgtcaaat
    26101 ttgcgccgat acatcgggac attttcatac gcgtgtttga gcgtccattt cagacgttgt
    26161 gtttgcaacg cctgtaactc atcaacagac gcggtttcaa tcgggtcaag ctttgtattg
    26221 gttatcattg tagggtactc gcaggttgat atgctcaaac acgctccaga atcatggcga
    26281 tgccctgacc gacaccgatg cacatggtgc acaatgcgta acgaccgtta cgccgatgca
    26341 gctcatggct ggcagccagt gccaggcggg caccactcat tcccaacgga tggcctaagg
    26401 cgatagcgcc tccgttgggg ttaacatgtg gggcatcatc aggcagcccc aattcgcgta
    26461 gtacacccaa cgcctgggcc gcgaacgctt cgttcagttc aatcacgtcc atatcgtgaa
    26521 tactcagccc cgcgcgttcc agcacccggc gagttgcagg caccggacca agccccatca
    26581 ggcgcggttc caccccggcg gttgccatgg ctacgattcg cgcccgcggt gtcagtcctt
    26641 gcgctgctgc catctgttca ctggcaataa tcaacgcagc ggctccgtca ttcaccccgg
    26701 aggcattgcc tgcggtaatc accccattgg cacgaaatgg tgcttttaac ccacgtaact
    26761 gttccagcgt cgtttccggg cgcagatgct catcatgttg tatttctgtt acaacgcctt
    26821 tcttgttttt caacacaacc ggaacaatct cctcagccag aatgcctgag gattgcgctt
    26881 ttgccgtacg ttgctgactg cgtagcgcaa aactatcttg atcttctcgc gatattttta
    26941 acagttcagc tacattctct gccgtttccg gcatgctgtc agttccaaat tgttgagcca
    27001 tgagcgggtt cacaaatcgc cagccaatag tggtatcaaa catctcagcc tgacgagaaa
    27061 atgcactggt tgccttgccc ataacaaacg gtgcccgaga cattgactcc acgccaccag
    27121 cgatcagcaa atcgccatcg cccgctttaa tcgcccgtgc ggcgaacccc agtgcgtcca
    27181 gcccggaacc acacaagcgg ttaatggttg tgccggaaac actctgcggc agccccgcca
    27241 gtaacgtcgc catccgggct acgttacggt tgtcttctcc cgcctgatta gcacagccga
    27301 ggatcacatc atcgatacac tccgcatcga gacgcgggtt tcgcaccagc agctcccgca
    27361 aagggatagc agccagatcg tcagcccgaa cacctgataa tgccccacca tagcggccaa
    27421 ttggcgtacg aattccgtca cagataaagg cttcacgcat caggcttctc ctgtaatggt
    27481 gccgccgatg cggtgagatt taccgcgaaa cagcgcaacc gttttttgtt gttggttaac
    27541 aatttcgatg tcgtaaacac cggtttgctt gccctgatga cgtacctgcg cagtagcagt
    27601 taaggtgtct ccggcaaagc ctggacgcaa aaaatcaatc gtgcaggcag aagcgacggc
    27661 tgccagcccc tggctattgc aggcgtaggc aaaggcagta tcagccagtg aaaatagctg
    27721 cccgccgtgg caactttgat gaccgttaag catttgtgca gtgacggtca tggtcactac
    27781 agcaaagcct tcatccattg agataatgtc gatgccaagc gctttggcgc aggcatcgtt
    27841 ctcatacatt gcatgggcat tttgccaggc cttatgactc ataaccgctc tccagaagcg
    27901 ctcgttggcg cagcaatgaa catgggcgat agcgttcttc gccatagtga tgttgtagat
    27961 tttccaagag ccttaatatt cgctgccagc caagttgcgc tccccaggca agcgggccat
    28021 atggataatt caccccaaga cgcatggcgg tatcgatatc ctgttcagag gccacgcctt
    28081 tttgcagcgc atcaagggct tcattgatga tcattgctac cgttcgccaa atcagcattc
    28141 ctgggtaatc tgcaatttgc agcactgttt tgccctgctg ttgcaggtaa taaatggcct
    28201 tgcgggtcgc tgagtccggg ttcaccgctg ccgcagcaat ggtcaccacc ttgcccgcca
    28261 ttttgtcgac cactaccacc gggcgtgcca gtcgtattgc cagcgcctgt gccgtctcgc
    28321 cttgtgtctc aatcaataaa acatcgtcaa tttccgtgac gccgtcactt ttcttttcta
    28381 ctttcattgg gctaaaactg tcgcttaccg cttccaggcc aacaactgcc tcacgttccg
    28441 cgcgccagtc gtacacgccc agcccacttt tcttgcccaa ccgtccacca atcaccagtt
    28501 cctgttgcac cagcgaaggt aaaaaacgac gctcctgcca gaaagcgtta aacaccgaac
    28561 aggtgacagc aaaattgacg tcctgaccaa tcagatcggt taattccagc ggccccatcg
    28621 ggaaaccagc gccatcgcga agtgcagcgt caatcacttc tggtgcagca acctgctctt
    28681 ccagtgcccg ccaggcctcg gaataataag gacgcgccac acggttaacg ataaatccgg
    28741 gggtcgaatg acagcgcact ggctgcttac cccaactcaa tgtcagttca cacaactgct
    28801 cgacaacttc cgctgccgtt gccagcccac tgaccacctc caccaacttc atcaccggtg
    28861 ccgggttaaa aaaatgcagc cccgcaacac gttcaggatt ttttacctcc gcagcaatcg
    28921 cggttataga gattgacgaa gtgttagtgg tcaatagcgt ttgtggcggg caaacttccg
    28981 ccagttgtgc aaagagcgct tttttaactt ccagacgttc agacgccgct tcaataacca
    29041 gattcgctgc tgccagcgcg tgaatatcgg tcaccgggat caggcgtttc aatgtgcgtt
    29101 cacaggtttc agcagtcagt tttccccgcg tcacgcgtga atttagccgc gcgtgtatcc
    29161 cgtcgattgc gcgggtcagc gcttcagcag aaatgtcata cagtaaaacc tgatgtccat
    29221 gactggcggc aacttcagca atgcctgccc ccatggtgcc gctcccaatc actgccacag
    29281 tttgcacatt tatcatcatg ctatttcccc gtgaactgcg gtgagcgttt agccaggaac
    29341 gcactgacac cttcacgata atctgcgctc cgtccggcaa gtcgctgata gtcacgttcc
    29401 agatccagtt gcgtatcgag cgtattggtt tcagcgctat ttatcgcttg cttgataagt
    29461 ccaagaccaa atgtcggttg tgtcgccaga tgccgtgcca gctgttgcgc ggtatctgcc
    29521 agcgtttcat catcaacaac ctgccagatc atcccccatt cgtgcgcctg ttcagcactc
    29581 agttgattcc ccagcagtgc cagccccatg gcgcgcgctc gcccagcaac gcgtggcagt
    29641 aaccaggttc caccgcaatc gggtactaaa cctaacttac taaacgccat gacgaatttt
    29701 gctgaacggg cagcaataac gatgtcgcat cccagtgcca gtgttgcgcc agcccctgcc
    29761 gccacgccat tgactgcaca gatcaccggt tttggcagtt ttgccaggcg acgtaccagt
    29821 gggttataga aacgttcaac tgacatccct aaatccggtg cggggcctgt gggatcaacg
    29881 ttacgatcgt taagatcctg accagcacaa aatccgcgcc cggcacctgt aagtaacagg
    29941 caacggatag tgtcgtcgcg ctcgacctgt ttcaggcact ctgccagttg tgcgtgcatc
    30001 tcatcattaa aactgttcag gcgttccggg cggttgagcg ttagtgtcat cacgcccttt
    30061 tctacatgac tgaggatgaa ttccatcatt agcgtccttt aaagtcgggc gtgcgttttt
    30121 gcaagaaagc ggagatgcct tcatgacgat cttctgttgc cgccagcaag gtgaataact
    30181 gtcgctcctg ggcaagtccg gcttgcaaag ccacttcctg cgactggcgc agcgcttgct
    30241 ttgccgcttg taaggccagc ggcgagtgac gtgccatttt cgacgccagc tgtaaggcgt
    30301 attcgagggt taaatcgctg gggaagacgt cgctaaccag cccggcctgc tgtgcttgct
    30361 gagcggtgat actttctccg ctcagcacca ttttgctggc taacgattta ccgacactac
    30421 ggattaaacg ttgcgttcct cctgcgcctg gcatgatgcc gagagtgatt tccggcaaac
    30481 caaaacgcgc gttctctccg gcaaccacca catcgcacaa caatgccagt tcgcaacccg
    30541 caccaagcgc gtaaccgttg acggctgcga tgagaggttt gttgaaggcc tgcaatcgcg
    30601 cccatagctg cgggcgtgta tcgtttaagg tggccgcgag atctttttct gccatttcgt
    30661 tgagatcggc cccagcggca aaaaagcgtg cattaccggt aatcacacag accgaaatgc
    30721 tgctatcggt agccgcagct tccagttcat ttaccagttg cgtcagaagg gcattattta
    30781 gcgcattacg tgcggcggga cggttaaggg tcagcaacaa tacttgttgc tgacggctga
    30841 cgatcagttc gctcatgcca tccccttcgc gtcaaagtca accaccacat cgctggtcag
    30901 cggcagtgcc tggcaactca acacatagcc tgcggccagt tcatccggtt ccagactgta
    30961 attggtttcc atcgccactt tgccacgcag cactttgcat ttgcaggtcg cacagacgcc
    31021 gcctttgcag gcatagggca gatcagcccc ctggcgcaat gccgcatcga gaatgctttc
    31081 atcgtcggca ttaagcacga tttcccgatc ccgcccatcc tgacgtacag tcactttttg
    31141 tccgtcactt tgcacgttaa cgctacgttt gacgcgcgtg ccaggcgtat taaaccgctc
    31201 cagatgaatg gttttatctg gcattcccag tgcttttaag gcggcttccg tctcatccat
    31261 catcgccgcc ggaccacaaa taaatgcctc atcataaaga cgaaaattaa tgagcgaggc
    31321 cccaagtgac tgtaattttt caccgtcaat acgcccgtga agcagatcgc tgtcgagggt
    31381 ttcctgactg aaaatgcaca acaactgtaa acgctgagga tatttgtctt tcaggtctgc
    31441 cagtgcctgg cgaaacatca tgctctggct ggtacggtta ccgtagatca gggtgaactg
    31501 actttcaggc tcggtttgta aagtggtggc gataatcgcc agcattggcg taatacctga
    31561 tcctgctgca atagccagat agcggccctg gcgttcggcc tgtggctgat agccgaaatg
    31621 cccctgcggc accatgacct ccagcgtcat accctggcgg atgtgttcgc gggcatagcg
    31681 ggagaaacgt ccgccttcaa tggctttcac cgccacacta atttcgccag gcagatagct
    31741 gcggcaaatg gagtaacaac ggcgtaattc ttcaccatca aagctggctt ttaaggtcaa
    31801 atgttgaccg gggcgaaagc gatacgcctc ctgcaaaggc tggggcaccg caaaggtaat
    31861 ggtcaccgca tcacgggttt ccggctccac ttttgccacc gttaaggaat gaaacgttgt
    31921 catggcatcc tcaaatacat ttgaaataat cgaaaggttc gcggcaacta tcgcagcgat
    31981 acaatgcttt gcaggccgtg gaaccaaatt cactgataag tgtggtatgg acgctggcgc
    32041 agcgcgggca acgtacttct ggcggcaaat gggcatggca actgtgtccg gcgggcgggc
    32101 taatgccata ctgtcgcaga cgctcacggg catcgggggt catccagtcg gtggtccatg
    32161 ccgggtcgag ttgcagcaca acctgaacgg gggtaaaccc gtgggttgtc attgcctcac
    32221 gtatcgcgcc aatcaaatgt tccgttgccg gacaaccgga ataagtcggc gtaaagccga
    32281 tcacccatcc ttctcccatc tgtgtcacat tacgcaccat gcctaaatcg gtaatggtca
    32341 gcaccgggat ctccgggtcc gggatctggc tgagtagcgc ccatatctca tgaacctgtg
    32401 gcggtgcaat ggtggccaga cgttgcatac ccatctcctc tgttaccatt gctgaccggg
    32461 caagacacgc tggagatact gcatttctgc cagcatgggt ccaaggtgtt cggtatgcag
    32521 tcctttttta ccgccagtgc gatacgcctg ctcttgcggt acgttcaatg tggcttcgtt
    32581 gatcccggca aaaacttcgg cttcccacgc tgcgcgtaaa gtgcgtggat caaccgcaat
    32641 accctcttca ctcagtgcaa tatcaatctc gtcggcatcg aacagttcgg cggtaaaacg
    32701 ccacaacttg ttgatggcct gctgcatctt ttgccctgat acgtcagtac cattgcccag
    32761 tcgctccagc cagccacgac taaaacgcag gtgataacgc gcttctttaa ttgctttggc
    32821 agaaatcgcc gccagttgcg gatcacggct ttccatcaga cgggtaaaga gcgccacatg
    32881 ccatgcatcg atgaaatact ggcgtgcaat ggtgtcggca aaattgccgt ttggttgttc
    32941 aaccaacaat aagttgctga actggcgctc gtctcgggtg aaggccaggg tatcttcatc
    33001 accttctccc gctaattcag cggcatacga taagaagtta cgtgcctgac ctaataaatc
    33061 gaggccaatg tttgccagtg cgagatcgat ttccagttcc ggtgcgtgac cgcaccattc
    33121 ccccaaccgc tgggagagca ccagacagtt atcgcccagg cgcaaggtgt aagccgttaa
    33181 ctgattcatt tccgacctca catgtgctca atgccatcag ggatggtgta aaacgttgga
    33241 tggcgataga ccttgctttc agccggatca aaaaattcac cgcgttcttc cggttgcgag
    33301 gcaacaattt cactcgcctt caccacccaa attgaacatc cttcgctacg acgggtgtaa
    33361 gcatcacggg cattttccag tgccatccgc tcatcggcag catgtaaact gccaacatgg
    33421 cggtgtgata agccctgttt gccacgcacg aaaacttcgt ataacggcca gtaaacatta
    33481 ctcattttga atctccttat gcgaccttac gggcaagttg tttttgtgca tgggccagcg
    33541 ctgcttcccg tacccaggta ccttcttccc aggctttacg tttggcgtcg agcctctcct
    33601 gattacaaat tccgcgaccg ttaattactt cgttaaactc ctgccagtcg atctcaccaa
    33661 agcggtagtg accgctttca gtgtcaaaat gcagatccgg gtcaggaacg gtcataccga
    33721 gcatttcaac ctgtggaacg gtgttatcca cgaaacgctg gcggagttcg tcgttggtga
    33781 aacgtttgat tttccaggcg agacttctgg cgctgtttgg cgagttatca tcgtttggcc
    33841 cgaacatcat taaggctggc caccagaaac ggttaatggc gtcttgcaac atctgctttt
    33901 gagcttcgct gccctgcgcc agtgccatgc aggcttcaaa cccctgacgc tggtgaaaac
    33961 tctcttcttt gcagattttc accattgctc tggcatacgg gccataagaa gtgcggcata
    34021 acgccacctg attgacgatc gctgcgccat ccaccagcca gccgataaca ccgatatcag
    34081 cccaactcaa tgtcggataa ttaaagatgg aggaatattt catccgtccg tcgagcattt
    34141 tttggtagat gtcttctctg gcgcagccca gtgtttcggc ggcgctatag agatacagac
    34201 catgaccggc ttcatcctgc actttggcca acagaatggc tttacgccgc aaggttggtg
    34261 cgcgagtgat ccagttacct tcaggcaaca taccaacaat ctcggagtgc gcatgctgcc
    34321 caatctggcg gatcagtgtc tttcggtaag catcgggcat ccagtcctgc ggctcgatag
    34381 ccgtttcctg ggctatccgt tgctcaaagc gttcttcttg ggtcacgtca tcacctttac
    34441 gattccagtt ttcgtgaatt aattaaaaac acgaatcacg ttgttttatg aaagttacac
    34501 aatggttaac taaaatcaca aattttgtat gcttgaactg tgacggattt cgcaccctat
    34561 ttttataaat cattatttat cagtgcatta atgttttccg cagttgctat acagatcgca
    34621 tagttaacat ttcgttaaaa gaacccttgc tttttatgat tcgcgattta actattagca
    34681 acagaaatgt gaaacatctg gagagtagcg atgcagcagt tagccagttt cttatccggt
    34741 acctggcagt ctggccgggg ccgtagccgt ttgattcacc acgctattag cggcgaggcg
    34801 ttatgggaag tgaccagtga aggtcttgat atggcggctg cccgccagtt tgccattgaa
    34861 aaaggtgccc ccgcccttcg cgctatgacc tttatcgaac gtgcggcgat gcttaaagcg
    34921 gtcgctaaac atctgctgag tgaaaaagag cgcttctatg ctctttctgc gcaaacaggc
    34981 gcaacgcggg cagacagttg ggttgatatt gaaggtggca ttgggacgtt atttacttac
    35041 gccagcctcg gtagccggga gctgcctgac gatacgctgt ggccggaaga tgaattgatc
    35101 cccttatcga aagaaggtgg atttgccgcg cgccatgtac tgacctcaaa gtcaggcgtg
    35161 gcagtgcata ttaacgcctt taacttcccc tgctggggaa tgctggaaaa gctggcacca
    35221 acgtggctgg gcggaatgcc agccatcatc aaaccagcta ccgcgacggc ccaactgact
    35281 caggcgatgg tgaaatcaat tgtcgatagt ggtcttgttc ccgaaggcgc aattagtctg
    35341 atctgcggta gtgcgggcga ccttttggat catctggaca gccaggatgt ggtgactttc
    35401 acggggtcag cggcgaccgg acagatgctg cgagttcagc caaatatcgt cgccaaatct
    35461 atccccttca ctatggaagc tgattccctg aactgctgcg tactgggcga agatgtcacc
    35521 ccggatcaac cggagtttgc gctgtttatt cgtgaagttg tgcgtgagat gaccacaaaa
    35581 gccgggcaaa aatgtacggc aatccggcgg attattgtgc cgcaggcatt ggttaatgct
    35641 gtcagtgatg ttctggttgc gcgattacag aaagtcgtgg tcggtgatcc tgctcaggaa
    35701 ggcgtgaaaa tgggcgcact ggtaaatgct gagcagcgtg ccgatgtgca ggaaaaagtg
    35761 aacatattgc tggctgcagg atgcgagatt cgcctcggtg gtcaggcgga tttatctgct
    35821 gcgggtgcct tcttcccgcc aaccttattg tactgtccgc agccggatga aacaccggcg
    35881 gtacatgcaa cagaagcctt tggccctgtc gcaacgctga tgccagcaca aaaccagcga
    35941 catgctctgc aactggcttg tgcaggcggc ggtagccttg cgggaacgct ggtgacggct
    36001 gatccgcaaa ttgcgcgtca gtttattacc gacgcggcac gtacgcatgg gcgaattcag
    36061 atcctcaatg aggaatcggc aaaagaatcc accgggcatg gctccccact gccacaactg
    36121 ttacatggtg ggcctggtcg cgcaggaggc ggtgaagaat taggtggttt acgagcggtg
    36181 aaacattaca tgcagcgaac cgctgttcag ggtagtccga cgatgcttgc cgctatcagt
    36241 aaacagtggg tgcgcggtgc gaaagtcgaa gaagatcgta ttcatccgtt ccgcaaatat
    36301 tttgaggagc tgcaaccagg cgacagcctg ttgactcccc gccgcacaat gacagaggcc
    36361 gatattgtta actttgcttg cctcagcggc gatcatttct atgcgcatat ggataagatt
    36421 gctgctgccg aatctatttt cggtgagcgt gtggtgcatg ggtattttgt gctttctgcg
    36481 gctgcgggtc tgtttgtcga tgccggtgtc ggtccggtca ttgctaatta tgggctggaa
    36541 aacttgcgct ttatcgaacc cgtaaagcca ggcgatacca tccaggtgcg tctcacctgt
    36601 aagcgcaaga cgctgaaaaa acagcgtagc gcagaagaaa aaccaacagg tgtggtggaa
    36661 tgggcggtag aggtattcaa tcagcatcaa accccggtgg cgctgtattc aattctgacg
    36721 ctggtggcca ggcagcacgg tgattttgtc gattaatcgg tgaatgaagg gcaacggcga
    36781 atagttgccc ttttatttca ccaaattttg tgacgttgtc acgttatgca tgatgtgtat
    36841 ctgtattttc agggcaccca ctgtatgaaa agctggcaca cctgccaaac aacctggcag
    36901 gtgcaggcaa tcccctttac atcagtactg ataatgtgaa cctgactaaa ccgcccacag
    36961 agcgcggttg ctaacaagaa cacaacatct gacgaggtta ataatgggaa gcccctctct
    37021 gtattctgcc cgtaaaacag ccctggcgtt ggcagtcgcc ttaagtttcg cctggcaagc
    37081 gccggtattt gcccacggtg gtgaagcgca tatggtgcca atggataaaa cgcttaaaga
    37141 atttggtgcc gatgtgcagt gggacgacta cgcccagatc tttaccctga ttaaagatgg
    37201 cgcgtacgtg aaagtgaagc ctggtgcgca aacagcaatt gttaatggtc agtctctggc
    37261 actgcaagta ccggtagtga tgaaagacaa taaagcctgg gtttctgaca cctttattaa
    37321 cgatgttttc cagtccgggc tggatcaaac ctttcaggta gaaaagcgcc ctcacccact
    37381 taatgcgcta actgcggacg aaattaaaca ggccgttgaa attgttaaag cttccgcgga
    37441 cttcaaaccc aatacccgtt ttactgagat ctccctgcta ccgccagata aagaagctgt
    37501 ctgggcgttt gcgctggaaa acaaaccggt tgaccagcca cgcaaagccg acgtcattat
    37561 gctcgacggc aaacatatca tcgaagcggt ggtggatctg caaaacaaca aactgctctc
    37621 ctggcaaccc attaaagacg cccacggtat ggtgttgctg gatgatttcg ccagtgtgca
    37681 gaacattatt aacaacagtg aagaatttgc cgctgccgtg aagaagcgcg gtattactga
    37741 tgcgaagaaa gtgattacca cgccgctgac cgtaggttat ttcgatggta aagatggcct
    37801 gaaacaagat gcccggttgc tcaaagtcat cagctatctt gatgtcggtg atggcaacta
    37861 ctgggcacat cccatcgaaa acctggtggc ggtcgttgat ttagaacaga aaaaaatcgt
    37921 taagattgaa gaaggtgcag tagttccggt gccaatgacc gcacgcccat ttgatggccg
    37981 tgaccgcgtt gctccggcag ttaagcctat gcaaatcatt gagcctgaag gtaaaaatta
    38041 caccattact ggcgatatga ttcactggcg gaactgggat tttcacctca gcatgaactc
    38101 gcgcgtcgga cctatgatct ccaccgtgac ttataacgac aatggcacca aacgcaaagt
    38161 catgtacgaa ggttctctcg gcggcatgat tgtgccttac ggtgatcctg atattggctg
    38221 gtactttaaa gcgtatctgg actctggtga ctacggtatg ggcacgctaa cctcgccaat
    38281 tgcacgtggc aaagatgcac cctctaacgc agtgctcctg aatgaaacca tcgccgacta
    38341 cactggcgta ccgatggaga tccctcgtgc tatcgcggta tttgaacgtt atgccgggcc
    38401 ggagtataag catcaggaaa tgggccagcc caacgtcagt accgaacgcc gggagttggt
    38461 ggtgcgctgg atcagtacag tgggtaacta tgactacatt tttgactgga tcttccatga
    38521 aaacggcact attggcattg atgccggtgc tacgggcatc gaagcggtga aaggtgttaa
    38581 agcgaaaacc atgcacgatg agacggcgaa agatgacacg cgctacggca cgcttatcga
    38641 tcacaatatc gtgggtacta cacaccaaca tatttataat ttccgcctcg atctggatgt
    38701 agatggcgag aataacagcc tggtggcgat ggacccggtg gtaaaaccga atactgccgg
    38761 tggcccacgc accagtacca tgcaagttaa tcagtacaac atcggcaatg aacaggatgc
    38821 cgcgcagaag tttgatccgg gcacgattcg tctgttgagt aacccgaaca aagagaaccg
    38881 catgggcaat ccagtttcct atcaaattat tccttatgca ggtggtactc acccggtagc
    38941 aaaaggtgcc cagttcgcgc cggacgagtg gatctatcat cgtttaagct ttatggacaa
    39001 gcagctttgg gtaacgcgtt atcatcctgg cgagcgtttc ccggaaggca aatatccgaa
    39061 ccgttctact catgacaccg gtcttggaca atacagtaaa gataacgagt cgctggacaa
    39121 caccgacgcc gttgtctgga tgaccaccgg caccacacat gtggcccgcg ccgaagagtg
    39181 gccgattatg ccgaccgaat gggtacatac tctgcttaaa ccatggaact tctttgacga
    39241 aacgccaacg ctaggggcgc tgaagaaaga taagtgattg tttcagacaa aaaaacggca
    39301 ccaggtgccg ttttttactt atgagcgaac cagattaata ccgtacacac accgacttag
    39361 tttcacacca accgtccagc cagtcggggc caaaatcacg gcccgttcct gactgcttca
    39421 tcccaccaaa cggtaagtta gcgtcaatta aggtatggct gtttacccac accgtccctg
    39481 cctgtaagcg atcgctatat tccagagcct gggagagatt ttgcgtccag acactggcag
    39541 ttaagccata ttccgtgtcg tttgccagtt gtaacgcctc ttctccatcc gctactcgca
    39601 ccaggtttac caccggacca aacacctctt cacgagttaa gcgcaattta gcatcgggat
    39661 ttaccaccag cgttggcgca acataatacc cctctccggc tggtccattc gacccgcgaa
    39721 tcagctctgc ttgctgtgcc tgcgcatcgt cgaggaatga acacactttg tcgcagtgcg
    39781 cacgagaaac caaagggtta atctgtgcaa caggtgacat ccccggtccc acttgcaacg
    39841 attttaccgc ctgctcaaat ccactaacca gcgtgtcaaa caacggcgct tcaatataaa
    39901 ttcgcgaact ggcggcgcat acttgccctt gattcaggaa gcttccggtc atcaagcctt
    39961 caataaccca ttgcggatca gcatctttta atacaattgc cgggttttta ccgcccagtt
    40021 ccagcgttac acgcgttaag tgatcagcag cagttctggc aatacctttt cccgtcgcgg
    40081 ttgaaccggt aaaactgatt ttcgcaacat gaggatgtga cgtcagggcc gcgccgcata
    40141 cagcacctga cccggtgacc acattaaaaa cgccatcagg gataccagcc tcgctggcca
    40201 gttccgccac gcgcaacatc gtcagtggcg tggtttccga aggcttaatc acgattgaac
    40261 agcctgctgc cagtgctggc atcaccttcc acataccaat catcaacgga aagttccatg
    40321 gcacaattcc cgccactacg ccaaccggct ctttacgcgt ccaggcctga taacgcgccc
    40381 cctggggtaa gggaatcgac aagtccagcg ttttacccgc gattttggtc gttaacccgg
    40441 cggtataacg catccagttc agcgtacagc ccacttcaaa agcacgggaa atggcaattg
    40501 acttgccttg ctccagggtt tccagttgcg ccagctcctc actgtgctgt tccaccagat
    40561 cggcaaaacg tagcagaata cgttcacgct ctgcgggtaa tcgcccggcc cagcgacgcg
    40621 agacaaaggc ccgccaggca gacatgactg cgttatctac atccgcttcg ttggcatcag
    40681 cagtagacgc aatttcttgc ccggtggccg gatcaaagat cgccaatcgt ttttcacttt
    40741 gtgcggggcc tggacgacca tcaatataaa gaccgtgttg acgatcgaga aactgttgga
    40801 cctggcttaa tactgctaca tgcggctctg tcatcacttt tccttattat ttacccagtg
    40861 tgatttcagt gtacgcagcg acaagaaaag tcactttttt cgctgtgaca ttcgctttgt
    40921 cagctgtgac aagctccgca aatcgtgaca ataaccgctt atgtctctat gcctgacaag
    40981 acttatttcc tatagtcagg ctcattagct tcgtttattg caacacaaat gcaacaataa
    41041 aaatacattt cacagagcga aaacgtgcca tgaaccccgc agtggataat gagtttcagc
    41101 aatggctttc ccaaatcaat caggtatgcg gaaattttac cggacgcctg ctgactgagc
    41161 gttacacggg tgtactggac acgcattttg ccaaaggact aaagctgagt accgtgacaa
    41221 ccagcggggt gaatttatcc cgcacctggc aggaagtaaa aggcagcgac gatgcctggt
    41281 tttacaccgt ttttcagctt agtggtcagg caataatgga gcaggatgag cgtcaggtgc
    41341 agattggcgc tggcgatatt acgttactcg atgcctcacg cccctgttcg ctttactggc
    41401 aggagtcttc taaacagatt tcattacttt tgccacgcac tctgctggaa caatattttc
    41461 cccatcaaaa acctgtctgc gcagaaagac tggacgctga cttacccatg gtgcaactca
    41521 gtcatcgcct gttacaggag agcatgaata atccggcact ttctgaaaca gaaagtgaag
    41581 ctgcgctaca ggcgatggtg tgtctgctgc gcccggtact tcatcagcgg gaatctgttc
    41641 aacctcgtcg tgaacgtcag tttcaaaaag tggttacgtt gatagacgat aatattcgcg
    41701 aagagatatt acgcccggag tggatagccg gagagacagg tatgtcagta cgtagtttgt
    41761 accgaatgtt tgccgataaa ggtttggtag tcgcgcaata tattcgtaac cgtcgtctcg
    41821 atttttgtgc agatgcgatt cgccatgccg cagatgatga aaaactggca ggcatcggct
    41881 ttcattgggg attttctgac cagagtcatt tttcaacggt atttaagcaa cgctttggga
    41941 tgacgccagg cgagtatcga cgtaaattcc gctaactaat tggcgttgtg tgtactgcaa
    42001 cgccaattag ttaaaacaga tatttgacta tgcatttttc agggcgaaag gcaatttgac
    42061 aggagttttt gaatattcac cttgtttcag gaaaatagat caatcgcttt ctcctgtagc
    42121 gttatcccga tattgcggtt ttgctattaa aattttcgca cccatttacc gtttatccct
    42181 tgtacatatt cccccggttt ggcccgagct acgagctttt gcccggcgag tttcgcgata
    42241 tcatctacag acacattatt ttgctttgcc agttgttggt aactcgcttt acgttcggca
    42301 ttaatgtcgg ttactaattt ctctgtttca gcatccgttt tcagcgcaac cagataacca
    42361 taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa tgccattaca
    42421 ttgctgctta ccagcccaac aagaaacgca caaagaagta atgttttctt catcatcgcc
    42481 tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca tctttgtctg ccttgatgat
    42541 gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttgg gcgcagcgac
    42601 ttcaatacgc ggcgtacagg cagccagcat aaaagatgac gtcaacgcag ccagtaaaat
    42661 tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg cagacgtgcg
    42721 ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca cagcgtaaaa
    42781 atattctctt catgatagta attaaggttt accgtaccgc ttttaccatc aacgcgactg
    42841 gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt aatttttgtc
    42901 gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc agtcatattg
    42961 tctttcacca ccgcatctgc ggtgtcttta tccagccgca aggtcattgg cccgctattt
    43021 gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg caatgcacca
    43081 ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag ttcgctggat
    43141 gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa ttgtttcatc
    43201 agtacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg ttcttcttca
    43261 gtccacggat aacgcccttg caaatcagcc gtaatatttt tcgctgtcac cagattaatc
    43321 acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc tccatcggca
    43381 aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat ccaggcacta
    43441 ccgcctttca acacgccgtg tcctcccgcg cggaatcctt gttcaggcgc agcagaaaat
    43501 gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg tggcaccagc
    43561 ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag acgaataccg
    43621 tcccagcgac catttacccg aaccgggcca atctcaccag catgtaaatc gcctttaaag
    43681 aggaaatacg tcggatcgcg cccatcgacg ctaaatttta aggttgatgg cggtaacaca
    43741 ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct gaaggaggga
    43801 tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg cttttctacc
    43861 gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga ggtcggtcag cgtaatcgtg
    43921 ctgtcatgcc attcaccttt tcctgcgaca tcccaggtgg cattcatcgg tgtaaaactg
    43981 ccttttcccc agtagcgcca ctgccagcgg cccgcgtcag ggagaaaatc attcgccagc
    44041 ccatccatat gcagcacgaa atcgcccagt tcattttcat gcgcctgcaa aatggcctgc
    44101 aaacgtccgt caacaccacg ttgggtgact tttacacccg ctaaaggcca gcggatttca
    44161 tcgatgtcca gcgaatcgat gactcttccc tttgaacgaa gtaacgcgcc tggctcaaag
    44221 gtcagcgttg ggtcagacag acttccactt agctgcgcag gtaaacgggc atataaaatg
    44281 aggtccgcct gtttcgcttc accggtcagc tgcagaggca gctgactgtt atccatgctt
    44341 aattttcctg ggccaaaatt tagcaccgcg ttgcctttac ccgcttgccc ctgggtcagc
    44401 acactcagtc gtccgctgat cagagcgttc tcaagccctg cctgccagtt gtcgactttg
    44461 acacccagtc ggccactcaa aggaaaacct gcatacggcc aactccagcg accatcgctt
    44521 acggtcaatt gttgacgagt aatttgccac ggcaaatcga gcaacggatc gccgttatcc
    44581 cgtgccagca caatcaattg cccgctattt tcctgccagt ccagctctgc atccaccagt
    44641 gacggttcct gcggcaagtt taacgtcgca gtagcatgcc cacttacagg aagtccatcc
    44701 ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg gcggctgatt ttcaaacgcg
    44761 acgacatcca gttcgctgac tgtaagttgt tgccctttca gctggccttg aaatttaact
    44821 ttttcgccct gataacgcag ttgctggata tcagaggtta atgcgagaga gagttttccc
    44881 tgccattcct gccagggaga aaaaatcagt ttatcgatat tgatccaggt gttaggcagc
    44941 atggactgcc actgcgcgag ggtttttgga gcagctggcg attgctccgt ctgcggcaat
    45001 ttcgccagac aagcagaatc aagttctacc atgccgacgt acaataacca gcggctggga
    45061 tgtgaaaggc tggcgttggt gatatgcgca agctgacaat ctcccaccag ataacggaga
    45121 tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt catctaatgc aatacgtgtc
    45181 ccgagcggta gccagatgcc cgccagcgtg ggaacccaca gcccgagcgt catcagcagc
    45241 gtcaacggca caagaataat cagtaataac agcgcgagaa cggctttata tttacccagc
    45301 atgggtagtt aatatcctga tttagcgaaa aattaagcat tcaatacggg tattgtggca
    45361 tgtttaaccg ttcagttgaa ggttgcgcct acactaagca tagttgttga tgaatttttc
    45421 aatatcgcca tagctttcaa ttatatttga aattttgtaa aatattttta gtagcttaaa
    45481 tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc acaaaacagt
    45541 acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg gaattttttg
    45601 actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg gtatgtattt
    45661 tcgtaaacga tgacggcagc cgcccggtgc tggaagagct gaaaaagcac ggcgttaaat
    45721 atatcgccct gcgctgtgcc ggtttcaata acgtcgacct tgacgcggca aaagaactgg
    45781 ggctgaaagt agtccgtgtt ccagcctatg atccagaggc cgttgctgaa cacgccatcg
    45841 gtatgatgat gacgctgaac cgccgtattc accgcgcgta tcagcgtacc cgtgacgcta
    45901 acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca ggcgttatcg
    45961 gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt atgcgtctgc
    46021 tggcgttcga tccgtatcca agtgcagcgg cgctggaact cggtgtggag tatgtcgatc
    46081 tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg acaccggaaa
    46141 actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg atgatcgtca
    46201 ataccagtcg cggtgcattg attgattctc aggcagcaat tgaagcgctg aaaaatcaga
    46261 aaattggttc gttgggtatg gacgtgtatg agaacgaacg cgatctattc tttgaagata
    46321 aatccaacga cgtaattcag gatgacgtat tccgtcgcct gtctgcctgc cacaacgtgc
    46381 tatttaccgg gcaccaggca ttcctgacag cagaagctct gaccagtatt tctcagacta
    46441 cgctgcaaaa cttaagcaat ctggaaaaag gcgaaacctg cccgaacgaa ctggtttaat
    46501 cttgccgctc ccctgcattc caggggagct gattcagata atccccaatg acctttcatc
    46561 ctctattctt aaaatagccc tgagtcagaa actgtaattg agaaccacaa tgaagaaagt
    46621 agccgcgctc gttgcgctaa gcctgctgat ggcgggatgt gtaagtaatg acaaaattgc
    46681 tgtaacgcca gaacagttac agcatcatcg ttttgtgctg gaaagcgtaa acggtaagcc
    46741 cgtgaccaac gataaaaatc cgccagaaat cagctttggt gaaaaaatga tgatttccgg
    46801 cagcatgtgt aaccgcttta gcggtgaagg caaactgtct aatggtgaac tgacagccaa
    46861 agggctggca atgacccgta tgatgtgcgc taacccgcag cttaatgaac tcgataacac
    46921 cattagcgaa atgctgaaag aaggtgcaca agtggatctg accgcgaacc agttaacgct
    46981 ggcgaccgcg aaacagacat taacttataa gctggcggat ttaatgaatt aatagctgcc
    47041 acagctcccg gcggcaagtg actgttcact acagcgtttg ccgttgggta atgcacacat
    47101 cccaatcgcc gtaccatcca gttgacgggc aacagaaagc gaaccgccga tcattgcaca
    47161 atttgcttct ccactactgg acatcgacgc ttttaaacct ggcgctacgt gcgccgcagt
    47221 cgcctgctga acaggttcac tactacacgc cgacaacaat aaagcggcac accctaccca
    47281 aaacgctgct cgcatctttt cttcctctga tcttcaagcc aaacgacacc gccataaata
    47341 ataggcagca cagagggcga cgtcgagagc tgtcctgcgc gttgccccgc atttttactt
    47401 ttttatggct atttttttgc cctctgtttg atcaaaacat tcattacgct gatgtggggg
    47461 acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa ttgaaggatt actactgggc
    47521 gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag ggtgtcatat gattactatt
    47581 gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca gtgaagttat cgccatctac
    47641 cctattaccc ccagttccac gatggcagaa caggctgatg cctgggccgg aaacggctta
    47701 aagaacgttt ggggagacac accacgcgta gttgaaatgc agtcggaagc gggtgctatc
    47761 gctaccgtgc atggcgcttt gcagacgggt gccctttcaa catcgtttac gtcatcgcag
    47821 ggtttgctgc tgatgatccc gacgctgtac aaactggcag gcgaactgac gccatttgtc
    47881 ctgcatgtag cggcacgtac cgttgccaca catgcacttt ctatttttgg cgatcattcc
    47941 gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt gtgcggctaa cgtccaggaa
    48001 gcgcaagatt ttgctctcat ttcgcacatc gcaacgctga agagccgcgt gccatttatt
    48061 catttctttg atggtttccg cacgtcccac gaaatcaata aaattgtccc gctggccgat
    48121 gacactattc ttgatctcat gccgcaggcc gaaattgatg ctcatcgcgc ccgggcgctc
    48181 aacccggaac atccggtgat ccgcggtacg tccgccaatc ctgacactta tttccagtct
    48241 cgcgaagcca ccaacccatg gtacgacgcg gtctatgacc atgttgaaca ggcgatgaat
    48301 gatttctctg ccgcgacagg tcgtcagtat cagccgtttg aatattacgg gcatccgcaa
    48361 gcggaacggg tgattatcct gatgggctct gccattggca cctgtgaaga agtggttgat
    48421 gaattgctaa cccgtggcga aaaagttggc gtgctgaaag ttcgcctgta ccgtcccttc
    48481 tccgccaaac acttgctgca agctctgccg ggatccgtac gcagcgtggc ggtactggac
    48541 agaaccaaag aacccggtgc ccaggcagaa ccgctctatc tggatgtaat gaccgcactg
    48601 gcagaagcct ttaataatgg cgagcgcgaa actctgcccc gtgtcattgg tgggcgctat
    48661 ggtctttcat ccaaagaatt tggcccggac tgtgtactgg cggtatttgc cgagctcaac
    48721 gcggctaaac cgaaagcgcg ctttacggtt ggtatttacg atgatgtgac caatctgtca
    48781 ctgccgttgc cggaaaacac cctgccaaac tcggcgaaac tggaagcctt gttttatggc
    48841 cttggtagtg atggcagcgt ttccgcgacc aaaaacaata tcaagattat cggtaattcc
    48901 acgccgtggt acgcacaggg ctattttgtt tacgactcca aaaaggcggg cggcctgacg
    48961 gtttctcacc ttcgcgtgag cgaacagccg attcgttccg cttatctcat ttcccaggct
    49021 gattttgttg gctgccacca gttgcagttt atcgataaat atcagatggc tgagcgttta
    49081 aaacctggcg gcattttcct gctcaacacg ccgtacagcg cagatgaagt gtggtcgcgc
    49141 ttgccgcagg aagttcaggc cgtgctaaat cagaaaaaag cgcgcttcta tgtgattaac
    49201 gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta ttaataccgt catgcagatg
    49261 gcttttttcc atctgacgca aattctgcct ggcgatagcg ccctcgcaga attgcagggt
    49321 gcgattgcca aaagttacag tagcaaaggc caggatctgg tggaacgcaa ctggcaggct
    49381 ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc aaccggtaaa tccgcacagc
    49441 gccaatcgac cgccagtggt ttccgatgcc gcccctgatt tcgtgaaaac cgtaaccgca
    49501 gcgatgctcg cagggcttgg tgatgccctc cccgtttcgg cgctgccgcc agacggcacc
    49561 tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg ccgaagagat ccccatctgg
    49621 aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg cttgcccaca ctcagctatt
    49681 cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc ctgccagcct gcattcgctg
    49741 gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct tgcaggtggc tccggaagat
    49801 tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga aagaccgtca gaatccagag
    49861 attaaagcca tcaatatgat gtcgcgcctg gaacatgtcg aagaagagaa aatcaattac
    49921 gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac tggaacgtat tgatattcgt
    49981 acatcgcagc tgattacacc gctgtttgaa tattcaggtg cttgctccgg ttgtggcgag
    50041 acgccgtata ttaaattgct gactcagctc tatggcgacc ggatgttgat cgctaacgcc
    50101 accggctgtt cttcaattta tggcggtaac ctgccctcta caccgtatac caccgatgcc
    50161 aacggtcgtg ggccggcatg ggcgaactct ctgtttgaag ataatgccga atttggcctt
    50221 ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc tgcgtctgct ggatcaattt
    50281 gccgataaaa tcccggcgga attactgaca gcgttgaaat cagacgctac gccagaggtt
    50341 cgtcgagaac aggtcgcagc tttacgccag caactcaacg atgttgccga agcacatgaa
    50401 ctgctgcgtg acgcagatgc actggtggaa aaatcaatct ggctgattgg tggtgatggc
    50461 tgggcttacg atatcggctt tggcggtctg gatcatgtat tgagtttgac ggaaaacgtc
    50521 aacattctgg tgctggatac gcaatgctat tccaacaccg gtggtcaggc gtcgaaagcg
    50581 acaccgctgg gtgcagtaac taaatttggc gagcacggca aacgtaaagc gcgtaaagat
    50641 cttggcgtca gtatgatgat gtacggtcat gtttatgtgg cgcagatttc tctcggcgcg
    50701 cagctcaacc agacggtgaa agcgattcag gaagcggaag cgtatccggg gccatcgctg
    50761 atcatcgctt atagcccatg tgaagagcat ggttacgatc tggcactcag ccacgaccag
    50821 atgcgccaac tcacagctac cggcttctgg ccgctatatc gcttcgatcc gcgtcgtgcc
    50881 gatgaaggca aactgccgct ggcgctggat tcacgcccgc cgtcagtagc actggaagaa
    50941 acgttacttc acgagcaacg tttccgtcgg ctgaattcgc agcagccaga agtggcagaa
    51001 cagttatgga aagatgctgc agctgatttg caaaaacgct atgacttcct ggcacaaatg
    51061 gccggaaaag cggaaaaaag caacaccgat taaatgctct ggataaggat tatccaattc
    51121 taaaaaaaag cccggacgac tgttcgggct tgtcttttta tatgttggaa aatcagtggc
    51181 aatgcaatgg cccaacagaa aataaaatct gtgacaataa aggcatataa cccgcgcaga
    51241 ataacgtata gaaaattaaa agtataattt ttattttgta tgtattatct tcgatacatt
    51301 caccagactt attatcattt cagaggaatt atttgattaa ggttttactt aaggcgtaac
    51361 aaatgatatt tatcggctaa ctgaacttct cctttaggat gttttcactc cccgaaatgg
    51421 ggataaaagg caaataaaat aactaaagga tttattcaat gaaaagcaaa gtactggcac
    51481 ttttaattcc tgccctgctc gccgcaggtg ctgcacatgc agccgaagtt tataataaag
    51541 acggcaacaa attagatctg tatggcaaag ttgatggcct gcattatttt tctgataatt
    51601 cggcgaaaga tggcgaccag agctatgcgc gtctgggttt taaaggcgaa acccaaatta
    51661 acgatcaact cactggctac ggtcaatggg aatacaatat tcaggcaaac aacactgaat
    51721 cttcaaaaaa ccagtcatgg acccgtctgg catttgccgg gctgaaattt gcagattacg
    51781 gttctttcga ttacggacgt aattatggcg taatgtacga catcgaaggc tggaccgata
    51841 tgctgcctga atttggcggt gactcttata ccaatgcaga caactttatg actggtcgag
    51901 ccaatggcgt cgcgacttat cgtaatactg atttcttcgg tctggtaaat ggtctgaact
    51961 tcgcggtgca gtatcaaggt aacaacgaag gtgccagtaa tggtcaggaa ggcaccaaca
    52021 acggacgtga tgttcgccat gaaaacggtg acggctgggg tctttccaca acatatgatt
    52081 taggcatggg ctttagcgct ggtgcggcat acacctcttc tgaccgcacc aatgatcagg
    52141 ttaaccatac tgcggcgggt ggtgataaag cagacgcgtg gactgctggg ctaaaatacg
    52201 atgctaacaa tatttacctg gcaaccatgt attcagaaac gcgtaatatg accccgtttg
    52261 gcgacagcga ttatgctgtt gcaaacaaaa cccagaactt tgaagtcact gcacagtacc
    52321 agtttgattt tggtctgcgt ccggcagtct ctttcctgat gtctaaaggc cgtgacctgc
    52381 acgctgccgg tggtgcagac aacccggcag gtgttgatga taaagatctg gttaaatacg
    52441 ccgatgttgg cgcgacttac tatttcaata aaaacatgtc cacttatgtt gactataaaa
    52501 tcaacctgtt ggatgaagat gacagtttct acgctgccaa tggcatctct accgatgata
    52561 ttgtcgcttt aggtctggtt tatcagttct aaatcctcct gcccgctgtt atggcgggct
    52621 ttttctgctt attcttcctc ctttgatcta aattaaaaat gtgaactccg tcattacaca
    52681 aaaagtgtca tctggcgtta cactttatgc ggatactaaa acaggaggtt ttatgaacag
    52741 aacgattctt gtccctatcg atatttccga ttcagaatta actcaacgcg tgattagcca
    52801 cgttgaggca gaggcaaaga ttgatgatgc agaggttcat ttcctgacgg taataccttc
    52861 actgccctac tatgcctctc tgggtttagc gtattccgca gaattaccgg caatggatga
    52921 cctgaaagcg gaagccaaat cgcaactgga agagatcatt aaaaaattta aactgccaac
    52981 cgacagagtg catgtccatg ttgaggaagg ctcgcccaaa gaccgcattc tggaaatggc
    53041 gaagaagatc cccgctcata tgatcatcat tgcttcccat cgaccggata tcaccactta
    53101 tctgctcggt tccaacgccg cagctgtagt gcgtcacgca gagtgctccg tgctggttgt
    53161 gcgctgacac taacgcccgc acattgctgc gggctttttg attcatttcg caaatgtgct
    53221 gacattttcc cctctaatcc gtaccataca cgccacagtt tttatatcag atttcttatg
    53281 ctgggcgttc cggcatgacg catactcttc tgatgccata taacgaattg agtcgctttt
    53341 aaatgtcgca aaatcaagaa attagtaaga aagaacaata caacctgaac aaattacaaa
    53401 aacgtctgcg tcgtaacgtg ggcgaagcca ttgctgactt caatatgatt gaagaaggcg
    53461 atcgcatcat ggtttgcctc tccgggggta aagacagcta taccatgctg gagattctgc
    53521 gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt ggctgttaac ctcgatcaaa
    53581 agcaaccggg cttcccggaa cacgttctgc ccgagtatct tgaaaagctg ggcgttgagt
    53641 acaagattgt tgaagagaat acttacggta tcgtgaaaga gaagattcca gagggcaaaa
    53701 ccacttgctc actgtgttct cgccttcgtc gcggtatcct ttatcgtacc gcaacggaac
    53761 tgggggcgac gaagatcgcg ttgggtcacc atcgtgacga tatcctgcaa acgttgttct
    53821 taaatatgtt ctacggcggc aagatgaaag gtatgcctcc gaaactgatg agcgatgatg
    53881 gcaaacatat cgttattcgt ccgctggcct actgccgcga gaaagatatt cagcgatttg
    53941 ccgatgcaaa agcgttcccg attattccgt gcaacctgtg cggttcacag cctaacctgc
    54001 aacgtcaggt gattgctgac atgttgcgtg actgggataa acgttatcca gggcgtatcg
    54061 agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca tctgtgcgat accaacctgt
    54121 tcgatttcaa aggcattacc cacggttctg aagtggttaa cgggggtgat ctggcgtttg
    54181 atcgcgaaga gatcccacta caaccggcgg gctggcagcc agaagaagat gaaaatcagt
    54241 tggatgagtt acggctgaat gtggttgaag tgaaataacc aggatagcgc ccgatgcgca
    54301 agcgtatcgg gctactctta tggaggccgg ataagacgcg gccagcgtcg catccggcaa
    54361 tcccgcataa gatgtttact cttgcacccg gcaattcaac atttcattat tttaataacc
    54421 gcacccggca cgtttttcct ttaatcttcc cgccctgtaa ctgtttccat gctttatgag
    54481 caacagcctg acgcaccgcg acatagacat gcgccggatg cacggcgatt ttgccaatat
    54541 ctgcgccatc aagcccgata tctcctgtca gtgcacctaa tacatcaccc gggcgcattt
    54601 tggctttttt cccgccatcg atacacaacg ttgccatttc tgcttccagc ggcacaatgg
    54661 aactattagc tggcggcgtt tgccagttaa gttttatctg caacatgtca gaaatgatat
    54721 tggcccgctg tgcttcttcc ggagcacaga aactgatcgc cagaccgcta tttcctgcac
    54781 gagctgtacg accgatgcga tgtacatgaa cttcagggtc ccacgccagc tcaaagttca
    54841 ccaccagctc aagcgattta atatccagac cacgcgcagc aacatcagta gcaaccagta
    54901 cacgggcgct accgttagca aaacgtacca gagtctgatc gcgatcgcgt tgctccagat
    54961 cgccgtgtaa cgacaatgca ctttgcccta cttcattcag cgcgtcgcag acagcctggc
    55021 aatctttttt ggtattgcaa aacaccacgc aagaggatgg ctgatgcaag cttaataacc
    55081 gttgcaacag aggaattttg cctttgctgg atgtctcata aaattgttgt tcaatgggtg
    55141 gcaaagcatc tgttgagtca atttcaatcg ccaaaggatc gcgttgcact cgtccgctga
    55201 ttgcggcgat ggcttccggc caggttgccg aaaacagaag cgtctgtcga gatgcaggcg
    55261 caaaacggat gacatcatca atggcatcgc taaatcccat atccagcatg cggtcggcct
    55321 catccatcac caaagtattc agcgcatcca gtgataccgt gcctttttgc aagtgatcca
    55381 gcaaacgacc cggcgttgcc acgataatat gcggcgcatg ttgcaacgaa tcacgctgca
    55441 taccgaacgg ttgaccaccg cataacgtca aaattttggt atttggcaga aaacgcgcca
    55501 gccgacgcaa ttcacctgcc acctgatccg ccagttcacg cgtaggacac agcactaaag
    55561 cctgggtttg aaatagcgac gcatcaattt gctgtaacaa gccgaggcca aaagccgccg
    55621 ttttgccgct gccggttttc gcctgcacgc gaacatcttt tccggcaagg atcgccggaa
    55681 gcgcggcggc ctgcaccggc gtcatggtta aataacccaa ctcattaagg ttcgtgagtt
    55741 gggcgggagg caaaacattc agggtagaaa aagcggtcac aatctattct cgtggtcatc
    55801 gacgcaaagt tagcaggcgc gtatcctcgc agatctacgc tcacgatgcg acaatttaat
    55861 cggttcttca tcaggtggtg ggtcaggcat gggttgcggg cgagggatcg gatcgggcac
    55921 tggaacagga tcgccaggaa tcggttcagg gacaggaatt tgcaaataaa taagtgtcgt
    55981 catatttccc tccggtcatt gggtggactc ttaaagggta gacgctgata aataacaggc
    56041 aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc aattgtgcta tgcagtaatt
    56101 caaaaaagga agtaagacaa tatggagcgc aacgcccatc gcttgacgtt gcattcacct
    56161 gcaagggaga tattgccctg aatgggtaga gagtttattg acttcgctca aactttgcgg
    56221 cgtttttgta tacagacagc cggaaaaatt gcttttgtta caaccattta ctacggtgca
    56281 accataaagc aacaccacca ataagaacaa ctaacagaat acaaaaaatt gaaaatccga
    56341 attgccaccc gccgccaggg atcccaccaa ggttgacgcc aaataaccct gtcagaaagg
    56401 tactgggtaa aaagaccatt gccatcaatg acattgtata ggtacgacga gctaaatttt
    56461 cctgcatcac ctgagcgatt tcatccgcca tcacgccagt ccgtgctata caggcgtcga
    56521 tttcgtcaag accgcgccca aggcgatcgg caatatcctg catccgacgg cgttggtcat
    56581 cgctcatcca cggcaaacgt tcactggcaa gacgagcata aacatcacgt tgcggtgcca
    56641 tatagcgacg catcacgatt aattgtttgc gcagcagagc caggaatcca cgcggtggaa
    56701 tttgctgatc aaggagatta tcttcaaggt cgataatttt atcgtgcagc tgctcgataa
    56761 attcgctgga atgatcggtc aacgcatcgc acacatccac cagccaccct ccgcaatcgg
    56821 tcggacccgt gccctcttcc agatcgctca ccacatcgtc cagcgccagc actttgcgtt
    56881 gtcgggtcga aacaattaac cgcccgtcca tatatacacg catggcgacc agttgatcgg
    56941 ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat cagcgtgcct tcaccgagac
    57001 ggctgactcg gggccgcgtg ctctcgcccg ccagcgcatc acgtacgtta ttgggaagca
    57061 gcggtgttgt cgccagccat tgggcgctat catggtgtac ataattaagg tggagccagc
    57121 agggatgcgc ttcatcaatc acatctgtat tttccagcgg tttaacgccg cctctaccat
    57181 ccagcatcca ggcaaatact gcatccggga cattaacgtc cgatccctta atcgcttcca
    57241 cagtgcctcc atcatcaacg cattattttg tagtctagcc ttctggccct gttacgcaac
    57301 atctcatcac cccattaccc tgaaatgatt aataaaattc tgtctaaatt gaatacaaaa
    57361 agcaaaatgc ttttccgtat acaaaccgtg tgaagtgtta aatagcgtct atcattatca
    57421 gaattatctg atcatatgac atggcttttt tgcgatcgga tagcaacaaa aattgataaa
    57481 aataacggga tctcaatgat tacgcacaac ttcaataccc tggacttact caccagtcct
    57541 gtctggatcg tttcgccctt tgaggaacag ttaatttatg ccaatagcgc ggcgcgactg
    57601 ttgatgcaag acctcacgtt tagtcagcta cgaaccggtc cctattccgt ctcctcacaa
    57661 aaagaactgc cgaaatacct ctccgatctg caaaaccaac acgatattat cgaaatcctc
    57721 actgttcagc gtaaagaaga ggaaacagca ttaagctgtc ggcttgtttt gcgaaagctg
    57781 acagaagcag aaccggtgat tattttcgaa ggtatcgaag cgccggcaac gctgggttta
    57841 aaagccagtc gctcggcaaa ttatcagcgc aaaaaacaag gtttttatgc gcgctttttt
    57901 ctgactaact ctgcaccaat gttgttgatt gacccctcac gagatggcca aatcgtcgat
    57961 gctaacctcg ccgcgctcaa tttctatggt tataaccatg aaacgatgtg ccagaaacat
    58021 acctgggaaa ttaatatgct cgggcgtcgc gtcatgccta tcatgcatga aatttcgcat
    58081 ttacccggcg gtcataaacc tttgaatttt gttcataaac tggcggatgg atctactcgt
    58141 catgtgcaga cctatgccgg gccgattgaa atttatggcg acaagctcat gttatgtatt
    58201 gtgcatgata ttactgagca aaaacggctg gaggagcagc tggaacatgc tgctcaccat
    58261 gacgcgatga ccggattact gaatcggcga cagttttatc acattacgga accaggccaa
    58321 atgcagcacc tcgccatcgc ccaggattac agcttgttgc tcatcgacac cgatcgtttt
    58381 aaacacatta acgatctcta tgggcattct aaaggtgatg aggtgttatg cgccctcgcc
    58441 cgcacccttg aaagttgcgc tcgcaaaggc gatttggtgt ttcgttgggg aggcgaagag
    58501 tttgtcttat tgctaccaag aaccccactg gataccgcgc tttcgctggc tgaaactatc
    58561 cgtgtaagcg tggcaaaagt gagtatttcg ggcttaccac gctttaccgt cagcattggt
    58621 gtggcgcatc acgaaggaaa tgaaagcatc gatgaactgt ttaaacgcgt tgatgatgct
    58681 ttgtatcggg cgaaaaatga tggacgcaac cgcgtgctgg cggcataagc cgcggatgcg
    58741 tctcgagatc aacgactgcg cttagcgtgg cgctcccagt tttcttgctt cgcctgcgcc
    58801 gttttacgta gtgcgacgta acacgccccg ctgccgccat gatgcggtag cgcagtgcaa
    58861 tatgcctgaa catcatcaaa ttcggtcagc cagcgcgcca catagctgcg gacaatattg
    58921 gcatgcgatt tatcatcccg tcctttgcca tgaataatca gcacgttacg caaaccatca
    58981 gccatggctt gttgaatgaa actgaacatc attttgcggc actcttccac cggctggcgc
    59041 aaaagattca ggctcgcctg ttgcggatat ttaccactgc gcagcttatc cagcacccca
    59101 tgttgcaacc cttcccgccg aaactccagc ggctgactta gtgggatgat gtcgagaaat
    59161 ccggtggtga ggaaattatc aagctgcagc gtgtcgatac gctgcggcgc acgttggtta
    59221 cgcgttggat gccagtggac atcggtagca cgtttcagcg gctggacatc ttccatggcg
    59281 tcaagaaaca gcgatttgtc gtcaaggttc atgttacatc ctcccgcaat taagagcgcg
    59341 atatgataac cagaccgggt cggtccaaca acgtattacc caaaattcca gtaataagtt
    59401 ccaaatattg ccgatatttt aagcaaaatg cttatgcatg attattcatt cacgatatta
    59461 ataatgtaac ttatattttc gtgaaatctg tcactgaaga aaattggcaa ctaaaggtta
    59521 aaaccgttat aacacagtca ccggcgcaga ggagacaatg ccggatttaa gacgcggatg
    59581 cactgctgtg tgtactgtag agtctggcgg atgtcgacag actctatttt tttatgcagt
    59641 tttaactttg cagatagccg cattctcgcc ggaattgcgt gattaatgac gcggtcagtg
    59701 gtgtctggcg actatcacgc cgctgaatca aataataggc cgctttcggt aaaatttcgc
    59761 taaccggcaa catcaccagc ccctgtccgt gcaagggatc gcagcccatt tcttcaggca
    59821 gtatgctgag aaaatcgctt tttgccacca gactgataca ggctgagaac gtctcgcaga
    59881 ctacaccgac ctgtggcgtt tgcgcctgat cgtcaagcaa ttcgctcaac tgtttgtagt
    59941 agctgccgtg tggcgtcggc attgtccagc tgtaatccag taactgtttg atcgaacggg
    60001 caccaatggc ggggtgtccc gggcggcaaa agatcgcgaa ttgcttttcc agtaatttct
    60061 caaaagtaaa ttcgtggtcg tacggtccct gataataggt attgatggtg aaatccaatt
    60121 ctccctgacg caattcatta atcatcgaca ccagttgccc ttccataatg cgtactttta
    60181 cctgcggatg ctgctgatga aaacgagata tgacagctgg catcagactg cgggaaatac
    60241 tggcccccat gccgatatta atctgccctg ccagttgccc ttgtcgttgg cgaatatcct
    60301 cttgggctgc gcgcagttct tcaagaataa gactggcgtg ctgataaaaa ctttcaccgg
    60361 catcagttaa cgtcacgcct ttactacggc gaaaaaagag ttgcgccgct aatccttctt
    60421 ccagctcctg aatagattta ctcagtgccg gttgcgacat attcaacatt cggctcgctc
    60481 cgcgaatgct gccctgacga gccacttcaa caaaagcacg aatttgatga atttttacct
    60541 gaaaagccat gacgccaccg ataaccgtta tttatcagac caaagaaact ggcatctact
    60601 ttaatgcaga tgattgtgtc agggtaattt atgaacggtt aaaactgtga aaaatcagtt
    60661 agtgataagt aaaaactatc gctacgtgaa ccgggtcaca ctttttactg atgacgggaa
    60721 aggttatgga gtctttgaat caatttgtta attcgcttgc cccaaaatta tcgcactggc
    60781 gacgtgattt tcatcactat gcagagtctg gctgggtgga attccgcact gccacccttg
    60841 ttgcggaaga attgcagcag ctcggctatt cactggcgct gggccgcgaa gtagttaatg
    60901 aaagtagccg gatgggacta cctgatgaac tcactctaca acgcgaattc gagcgcgctc
    60961 gtcaacaggg tgcgctagca caatggattg cggtttttga aggtggtttc actggtatcg
    61021 tcgccaccct ggataccggt cgccccggtc cggtgatggc tttccgtgtc gatatggacg
    61081 cgctggatct cagtgaagag caggatgtca gccatcgccc ctaccgcgac ggttttgcgt
    61141 catgtaacgc cggaatgatg catgcctgtg gtcatgatgg acataccgcc attgggcttg
    61201 ggctggcgca tacccttaaa caattcgagt ccggactaca tggcgtcatc aaactgattt
    61261 ttcagcctgc agaggaaggt acgcgtggcg cgcgggcgat ggtcgatgca ggtgtcgtag
    61321 atgatgttga ttattttact gccgtgcaca ttggcactgg cgtacctgcg ggcaccgtgg
    61381 tgtgcggcag tgataatttt atggcaacca ccaaatttga cgcgcacttc accggtaccg
    61441 ccgctcacgc aggcgcaaaa ccagaagacg gtcacaatgc cttgttggcg gcagcacaag
    61501 ccactcttgc actgcatgca atcgccccgc acagcgaagg agcttccaga gtaaacgtgg
    61561 gcgttatgca ggcaggaagc ggtcgtaacg ttgttcctgc ctcggcgttg ctgaaagtgg
    61621 aaacacgcgg ggccagcgac gtcattaatc aatatgtttt tgaccgtgca caacaagcga
    61681 ttcagggcgc agcaaccatg tatggtgtcg gcgttgaaac tcgtctgatg ggtgcagcta
    61741 ccgccagttc tccttcgccg caatgggtcg catggttgca aagtcaggcg gctcaggtcg
    61801 cgggggtcaa tcaggccatt gaacgtgttg aagcgcctgc gggttccgaa gatgccacat
    61861 taatgatggc ccgcgtgcag caacatcaag ggcaagcctc ctacgtggtg tttggcacac
    61921 agctggcggc aggtcatcac aacgaaaaat tcgattttga cgagcaggtt ctcgctattg
    61981 ccgtcgaaac gctggcgcgc accgcgctca attttccctg gacgcgaggt atctgatgca
    62041 ggaaatctat cgttttatcg acgatgcgat tgaagccgat cgccaacgtt ataccgatat
    62101 tgccgatcaa atctgggatc atccagaaac acgttttgaa gagttctggt cagcggagca
    62161 tctggcttcg gcgctggaat ctgcaggctt caccgttacc cgcaacgtag gcaatatccc
    62221 aaatgccttt attgcttcgt ttggtcaagg caaaccggtt atcgccctgc tgggagaata
    62281 tgacgccctg gcaggtttaa gtcagcaagc aggttgcgcg caacctacat ccgtgacgcc
    62341 cggtgaaaat ggtcacggtt gcggacacaa tttgctggga accgccgcct ttgccgctgc
    62401 aatagccgtc aagaaatggc tggaacaata tgggcaaggc ggcacagtgc gcttttatgg
    62461 ttgtcctggc gaagaaggcg gctcgggtaa aacgttcatg gtccgcgagg gggtatttga
    62521 tgatgtggat gcggcactca cctggcaccc ggaagccttt gccggtatgt tcaatacccg
    62581 cacgctggca aacattcagg catcatggcg ctttaaaggg atcgcagcac atgccgcgaa
    62641 ttcccctcat ttgggacgca gcgcccttga tgccgtaacg ttgatgacca ctggcaccaa
    62701 cttcctcaac gaacatatta ttgaaaaagc gcgcgtacac tatgccatca cagatagcgg
    62761 cgggatctcg cccaacgtgg tccaggcgca ggcagaagtg ctttatctta tccgtgcccc
    62821 cgaaatgacc gacgtgcagc atatttatga tcgggtcgcc aaaatcgccg aaggtgcggc
    62881 attgatgacc gaaaccacgg ttgaatgccg cttcgacaaa gcctgttcca gttatctccc
    62941 gaatcgcacc ttagaaaatg ccatgtacca tgccctatcc cattttggta ccccggaatg
    63001 gaactccgaa gaactggctt ttgcgaaaca aattcaggct acgctcaccc ccaacgatcg
    63061 gcaaaacagt ctgaataata tcgccgcaac cggtggcgaa aacggcaagg tttttgcact
    63121 acgtcatcgt gaaacggtac tggcgaatga ggtcgctcca tatgccgcca ccgataacgt
    63181 gcttgcggca tcgactgatg tcggcgacgt cagttggaaa ctgcctgttg cccagtgttt
    63241 cagcccctgt tttgccgtcg gtacaccgct acatacgtgg caactggtta gccaggggcg
    63301 aacatctatt gctcataaag gaatgctgct ggcggcgaaa actatggcag caaccacagt
    63361 caatctcttc attgattcag ggctattgca agaatgccaa caagagcatc agcaagttac
    63421 ggacacgcaa ccgtatcact gccctatccc gaaaaacgtg acaccgtcac ctttaaaata
    63481 acaacaacaa cgcaaacaca acaaccgagg aatgcccatg agtatgtcat ccataccgtc
    63541 gtcctcccaa tccgggaagc tctatggctg ggtcgaaaga attggtaaca aggttcccca
    63601 tccttttttg ctctttatct atttgataat cgtactcatg gtgacgacgg caattttgtc
    63661 agcctttggc gtcagtgcga aaaacccgac cgatggtacg ccggtggtgg tgaaaaacct
    63721 gctcagtgtg gaaggattac actggttttt acccaatgtg attaaaaact ttagcggttt
    63781 tgctccactt ggagcgatcc tggcgctggt tttaggtgcc ggcctagcgg agcgcgtcgg
    63841 cttactgcca gcgctaatgg ttaaaatggc atcgcatgtt aatgcccgct acgccagtta
    63901 tatggtgctg tttattgctt ttttcagcca catttcttcc gatgcggcgt tagtgatcat
    63961 gccaccgatg ggtgcgctga tttttctggc ggtgggcagg catccagttg caggtttact
    64021 ggctgccatt gcaggcgtag gttgcggctt tacggctaat ttactgattg tcacaaccga
    64081 cgtgttgctg tcggggatca gcacggaagc ggcggctgcg ttcaatccgc aaatgcacgt
    64141 cagtgtaatt gataactggt attttatggc cagctccgta gtcgtactga cgattgttgg
    64201 cggcctgata accgacaaaa tcatcgagcc acgattaggt caatggcagg gaaacagcga
    64261 tgagaaactg cagacattga ccgaaagtca gcgttttggt ttacgcatag caggtgtcgt
    64321 atcgctactt tttattgctg cgatcgcgct gatggtgatc ccggaaaacg ggatattgcg
    64381 cgatccgatt aatcacaccg tgatgccatc accctttatt aaaggtatcg tgccactgat
    64441 cattcttttt ttctttgttg tctcgctggc ttatggcatc gctacccgca caattcgacg
    64501 tcaggcggat ttaccgcatt taatgattga accgatgaaa gagatggcgg gatttatcgt
    64561 gatggttttt cccctcgccc aatttgtcgc catgtttaac tggagcaaca tggggaaatt
    64621 catcgccgtg gggctgaccg atatacttga aagttcaggg cttagcggca tcccggcgtt
    64681 tgtcggtctg gcgttgcttt cctctttctt atgcatgttt attgccagcg gttccgcaat
    64741 ctggtcgatt ctggccccca ttttcgtacc aatgtttatg ctacttggct ttcacccggc
    64801 atttgcgcaa atcctctttc gtattgccga ctcatccgta ttgcctttag cgccggtatc
    64861 tccttttgtt ccactgtttc ttggattcct gcaacgctac aaaccagacg caaaactggg
    64921 tacttactat tcgttagtct tgccctatcc gcttatcttt ttggtggtat ggctgctgat
    64981 gttgctggcg tggtatcttg ttggtctgcc gataggtccg gggatttacc cacgtttgtc
    65041 ttaagagaga acggatgctg agattacttg aagaaaaaat tgccacgcca ctgggtccac
    65101 tgtgggtgat ttgcgatgag caatttcgcc tgcgggcggt tgaatgggaa gagtacagcg
    65161 aacgcatggt gcagctgctg gacatccatt atcgcaaaga aggctatgag cgcatttctg
    65221 ccaccaaccc aggcggttta agcgacaagc ttcgtgatta ttttgccggt aatcttagca
    65281 ttattgatac gcttcccact gccacagggg ggacgccatt tcagcgcgaa gtctggaaaa
    65341 ctctacgcac tatcccctgc gggcaggtaa tgcattacgg ccaactggct gaacaattgg
    65401 gccgccctgg cgcggcgcgt gccgttggtg cggcaaacgg atcgaatccc atcagcatcg
    65461 tcgtaccttg ccatcgggtt attggccgaa acggcaccat gaccggatat gcaggcggag
    65521 ttcagcgaaa agagtggtta ttgcgccacg aaggttatct tttgctgtaa acattaaaca
    65581 atttgtgcca gcttgttcac acttttatgt aaagttaccc ttaacaactt aagggttttc
    65641 aaatagatag acatatattt acatctgata tcggaattct ctgctgttaa ggtttgctta
    65701 gacttacttg ctccctaaaa agatgttaaa attgacaaat atcaattacg gcttgagcag
    65761 acctatgatc ccggaaaagc gaattatacg gcgcattcag tctggcggtt gtgctatcca
    65821 ttgccaggat tgcagcatca gccagctttg catcccgttc acactcaacg aacatgagct
    65881 tgatcagctt gataatatca ttgagcggaa gaagcctatt cagaaaggcc agacgctgtt
    65941 taaggctggt gatgaactta aatcgcttta tgccatccgc tccggtacga ttaaaagtta
    66001 taccatcact gagcaaggcg acgagcaaat cactggtttc catttagctg gcgacctggt
    66061 gggatttgac gccatcggca gcggccatca cccgagcttc gctcaggcgc tggaaacctc
    66121 gatggtatgt gaaatcccgt tcgaaacgct ggacgatttg tccggtaaaa tgccgaatct
    66181 gcgtcagcag atgatgcgtc tgatgagcgg tgaaatcaaa ggcgatcagg acatgatcct
    66241 gctgttgtcg aagaaaaatg ccgaggaacg tctggctgca ttcatctaca acctgtcccg
    66301 tcgttttgcc caacgcggct tctcccctcg tgaattccgc ctgacgatga ctcgtggcga
    66361 tatcggtaac tatctgggcc tgacggtaga aaccatcagc cgtctgctgg gtcgcttcca
    66421 gaaaagcggt atgctggcag tcaaaggtaa atacatcacc atcgaaaata acgatgcgct
    66481 ggcccagctt gctggtcata cgcgtaatgt tgcctgattt ttccgcataa ctcactatcc
    66541 ttctgtcata tcattaaatt tttctgattt attgatctgg cagaaggttc atcactgttt
    66601 cattcaccag atatgggtta atcttttaat tacaaactgc gttgacagtt gttgtaagga
    66661 gaccctgtat ggctatgtat cagaacatgc tcgttgttat cgatcctaac caggacgacc
    66721 aaccagcatt gcggcgagct gtttatttac atcaacggat tggtggcaaa attaaagcct
    66781 ttttgccgat ctatgacttc tcatacgaaa tgaccaccct gctctccccg gacgaacgta
    66841 cggctatgcg tcagggcgtc atcagccagc gtacagcctg gatccacgag caggcaaaat
    66901 attatctcaa tgctggcgtt cccattgaaa ttaaagtggt ctggcataac cgtcctttcg
    66961 aagccatcat tcaggaagtg atcagcggcg ggcacgattt ggtgctaaaa atggcgcacc
    67021 aacatgaccg tctggaagcg gtgattttta cgccaacgga ctggcatctg ttacgcaaat
    67081 gcccaagccc ggtgtggatg gtgaaagacc agccgtggcc ggaaggaggc aaggcgctgg
    67141 tggcggtgaa tctcgccagt gaagagccgt atcataatgc gctcaatgaa aaactggtca
    67201 aagagacgat cgaactggca gaacaagtca accataccga agttcatctg gttggcgctt
    67261 atccggtaac gccaattaat atcgcgattg aactgccgga atttgatccg agcgtttata
    67321 acgatgccat tcgtgggcaa catttgctgg caatgaaagc tctgcggcag aaattcggca
    67381 ttaatgaaaa catgacgcac gtagaaaaag gtctgccaga agaggtgatt cctgatttgg
    67441 cggaacactt acaggcgggg attgtggttc tgggcacggt aggacgcacc ggtatttcag
    67501 cagcattcct cggcaacacg gcggaacagg tgattgatca tcttcgctgc gatctgttgg
    67561 tcattaaacc tgaccagtat cagacacccg ttgaactgga tgacgaagaa gacgattaac
    67621 gactcttcaa taactgacaa ggcccgagtt atcgggccta ttttttactt actgctcttc
    67681 gccgccaagg aaataaattc ccaacgggat tgccagcaac acagtaaaga ccaggctata
    67741 aacaaaaatc atcgatgact gaatcacata cattgatgtc gtccactcag acagcggaat
    67801 attgtactgt tctataacgc cgccgatagt cgcgcgagtc acaaccgacg ccgcaataat
    67861 gaaaaccgcc aacaaaccaa agagaaattt cttccctttc gctgacttca gtttcgccat
    67921 aatcatgatg taatccttgt gcagacaaat ttcttgttac ctaaacgtaa caataccatg
    67981 acatttcacc gttgtctgct ctgaaaacct cacctcttag ctggatttac aatcaacgca
    68041 ttgattagtt agctaatgtt gctacttcct tccctgacta ttgatgatct gaatgaattg
    68101 tcgcggtaaa atagccccgc caggggtttc cggcatgacg acaattatct ggaggagcct
    68161 gataaaacaa tgatcgctga actgtttaca aataatgcgc ttaatctggt cattattttc
    68221 ggtagctgcg cagcattgat tctgatgagc ttttggtttc gccgcggaaa tcgtaaaaga
    68281 aaaggttttt tattccatgc ggtgcaattt ttaatctaca ccataattat cagtgctgtt
    68341 ggtagcatta ttaattatgt tatagaaaac tataaactca aatttatcac cccaggcgtt
    68401 atcgatttta tctgtacgtc cctgattgcg gttattctga cgattaagct atttttgctg
    68461 attaatcagt ttgaaaaaca gcagattaaa aaaggtcgcg atatcaccag tgcgcggatt
    68521 atgtcgcgca ttatcaaaat caccattatt gtggtgcttg ttctgcttta tggcgaacat
    68581 ttcggcatga gcctttctgg cttgctgacc tttggtggta ttggtggtct ggctgtcggt
    68641 atggccggta aagatattct gagtaatttc ttttccggga tcatgctcta tttcgaccgt
    68701 cctttcagta ttggcgactg gatccgttca ccggacagaa atatcgaagg tacagtagcg
    68761 gaaattggct ggcgaattac caaaattacg acctttgata atcgtccatt gtacgtaccg
    68821 aactcgctgt tttcatcgat cagcgtagaa aacccaggac gaatgaccaa ccgccgcatt
    68881 accacgacca ttggtttacg ttatgaggat gcggcaaaag tgggcgttat tgtcgaagcc
    68941 gtacgtgaga tgctgaaaaa tcacccggcc atcgaccagc gacaaacctt actggtttat
    69001 ttcaaccagt ttgccgactc gtcattgaat attatggttt attgctttac caaaaccacg
    69061 gtatgggctg agtggcttgc tgcacagcaa gacgtttatt tgaagattat cgatattgta
    69121 cagtcacacg gcgcggactt tgccttcccc agccagacgc tgtatatgga taacattaca
    69181 ccgccggaac agggtcgcta aatcagacat cactggcgtt atccacaagg gtaacgccag
    69241 ttcccatcac atcaatgctt cacaatatac atagtccggc tgtacgccac atcttccggg
    69301 ttattaatgg gataaccttt cagccacggc ttgattaatc gtccattggt atattgataa
    69361 attggtgcaa tcggtgcttg ctccatgagg attttttctg ccgcgttgta atcggcatta
    69421 cgcgctttaa cggtattttc cgtcgatgcc tgggccagaa ctttgtcgta tgccggattg
    69481 ttaaagcgtg aaatatttcc tgaatgcgtt gacgttaata atgtcaggaa agtggagggt
    69541 tcattataat cccccaccca tgaggcacgg ataacatcaa aattgccggt gttacggcta
    69601 tcgatatagg ttttccattc ctgattttgc aatttaacat ctacgccaag gttctttttc
    69661 cacatcgatg ctacagcaat cgcaattttt tgatggtttt ctgaagtgtt atacaaaagc
    69721 gtcagcttca gcggtttttg cggaccataa ccagctgcgc tcaacaaagt ttttgcctgt
    69781 gcattcagtt cttcctgact catttgttca aacggcgaag gttccggcgt aaatccggcg
    69841 gtaacatctg gtgtaaaatg ccacgctggc ttttcgcccg ttcccaacac tttttcggtc
    69901 atcaggcggc gatctatcgt catacttaat gccaggcgaa cgcgctgatc tgccgtcggc
    69961 cctttttgcg tgttaaacgc ataataatag gtcccgagct gtggcggcgt ataaacctgc
    70021 cccggaatat ccttcaacag cttctgatac atatttttcg ggaaggattc ggtgatatca
    70081 atatcccccg cgaggtaacg cttagtggct gcggattcct gattaattgg caggaaggtc
    70141 actttttgca gtaccgtttt ggcgttatcc caataatggg tattcggtac cacgaccagt
    70201 ttttcattga ctacgcgatc tttaagaaca taagcgccat tgccgatcag atttccgggt
    70261 ttcgtccact ctttaccgct ttctacgttg gctttttgca ccgggaagaa ggcaaagtta
    70321 gcggttaaat tcacaaacca cggcaacggt ttatcaagct gaattttcaa ggtatgggca
    70381 tcaactgcgg tgacgccaag ctggtcaggc gtagctttac catcaataat cgcctgtgcg
    70441 ttgttgattc ccgccagcgc ggcaaaccat gcaaatggcg atagcgtttt cgggtccacc
    70501 agacgctgcc agctgtagac aaaatcttgc gccgttaccg gtgtgccatc cgcccatttt
    70561 gcgttatcgc gcagggtaaa agtccagata cggttgtcat tacttttcca ctgagtcgca
    70621 acgccgggga caatctcccc tttctcgttc tgattcacca gtccttcaaa cagatcgcga
    70681 atgacctgaa tctctggcag gcccacggct ttagcgggat ccagcgacgc aggctcatct
    70741 ttaatgtggc gcaccagctc ctgcttctct gccagtactg tgccgctcgg aacttctgca
    70801 gcatacgaaa gagaaatgct gctgaccaac agcgcacaac acgtgactga aacagagtgc
    70861 ttcataagtt tccctctaac cgacattaaa attgatgcgt aattatttgt ttcgccttaa
    70921 agaaatgcaa acaacttcca tcaaaaaagt gacacaggaa gcataaaatg ttttatgtat
    70981 caatatatta aatcagattt agtgtaaata atcgacgcca aaatgcggca aagcacatag
    71041 aacaccgaac actacgttca ggcttcgcac gttcaaacca gttttcggta tcaaaatcag
    71101 catctgtcgc cattgtacga agaataaaaa ataaccaaaa cgttgaaagg aactgagaat
    71161 acgttaagag agaaattaaa ttaatgtatc agcaagtagt gctaaacagc acctttatac
    71221 ttcagcctat ctataaataa tgagaatgcc gagccagcat gtcgacggtg tggatagtac
    71281 aggtgataac cgggtaaatc tggtgtgaat ttatccaaaa cacgtatcag ttttttttct
    71341 ttaatagccc gctcaacctg atcataaggt agatacgcca atccatgccc atcaattgca
    71401 gcatcaatga tcaggtctat cgtattcagt aaaagctgac cttccatgcg aacacgaact
    71461 tcacgtccac cgcgtattaa tctccagcga tttgctgtac ccgatgtggg aagatacaaa
    71521 ttaattgcct gatgatctat taattgtgac actgacgttg gaacacttcg gcgagaaaaa
    71581 taatccggtg agccaacaat agccattggt atatctggcc cgattcgaat ggcgatcata
    71641 tctttatcca tctccccacc cagacggacg cctgcatcaa aacgttcaga aacgacatcg
    71701 gtcaaaccat aatcaatggt gagctgaata tcaatttcag gatgcgattt caggaatgtg
    71761 cgcattgctg gtaacaatat cgtttttgct gcatgttcta cagtagtaat acgtattgtc
    71821 ccggatgggc ggttctgcag ctcgctcagg gatgccaggg ctgaatctat gtcatgcaac
    71881 atagggccaa gaacagacaa aagatgctcg cccgcttcag ttggaacaac gctgcgcgtg
    71941 gttcgcgtca gaagccgcaa tcccaatcgt tcttctatac gacgcactat ctggcttaaa
    72001 gctgactgcg ccatgctcag gcgggctgct gcacgagtga agctacgctc ctctgcaacg
    72061 acgacaaacg ccatcagatc agcgatttct tcacgcttca ttcagtatct ccatgcaatc
    72121 tattaatcat ttaaatgata ggtctaatca tgattttggg tctaatcaac ttaatgatca
    72181 atatctatag tgatatcacc aaccgttaaa acgaaccgac aactccatga ggaacggaat
    72241 gatgaataat aaagtcagct tcactaacag caataatcca accatctctt tgtctgcagt
    72301 gatctatttc cctccaaaat ttgatgaaac ccgtcagtat caggcaattg tggtatctca
    72361 cccaggtggg ggcgttaaag aacaaacggc cggaacctat gccaaaaaac tggcggaaaa
    72421 gggatttgtc actattgctt atgacgcatc ttatcaaggt gaaagtggcg gcgagccgcg
    72481 tcagttagaa aacccataca ttcgtactga agacatcagt gccgtgattg attacctgac
    72541 aacgctttct tatgtcgaca atacccgaat tggtgcgatg ggaatttgtg ccggtgcagg
    72601 atatacagcc aatgctgcaa ttcaggatcg ccgcatcaag gccatcggta ccgttagtgc
    72661 ggtcaatatt ggttcgatgt tccgtaatgg ttgggaaaac aatgtgaaat ctattgatgc
    72721 cctgccatac gttgaagcgg gttcaaacgc ccgaaccagc gatattagca gcggagaata
    72781 tgccgttatg ccactggcac caatgaagga gtccgatgcg ccgaacgaag aactgcgtca
    72841 ggcctgggag tattaccaca cccctcgcgc gcagtatcca acagcaccgg gttacgctac
    72901 tttgcgcagc cttaaccaga ttattaccta tgatgcttac catatggcgg aagtgtacct
    72961 gactcagccg atgcagattg tggcgggcag ccaggcaggg agcaaatgga tgagtgacga
    73021 cctgtacgat cgggcatcaa gccaggataa acgctatcac attgttgaag gcgcaaacca
    73081 tatggatctt tatgacggca aagcctatgt ggctgaagca atttcagtat tagctccgtt
    73141 ctttgaggaa actctgtaat gaaacatatt caaatccgca atagtgatat ggcctggcat
    73201 attgctgcca ataatctggg ataattttca tgaaaaatgt actgattctt ggtgctggcg
    73261 gtcagatagc ccgccatgtg attaatcaac tggcagataa gcaaaccatt aaacaaactt
    73321 tatttgcccg tcagccagca aaaatccaca aaccctatcc aactaataaa atgcaaacaa
    73381 cttccggcaa aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa
    73441 tccgcaaaat ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg
    73501 taacccgccc acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt
    73561 cattattggg cgcgccgtta atctggtttc ctgcccctgc agccagtcgc gaaagtggtt
    73621 tgattctggc tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc
    73681 tgcggacttt gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg
    73741 gctgccagct ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg
    73801 cggcgaactg gaaggaaggt gaaacagttt atcgctggaa cagcgccgct gaagaacgtg
    73861 atgtcgtatt actgacaggt gacaaacctg gctccgaacc tgaaaccctg gcgctgtgcc
    73921 agcttataca ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcttgta
    73981 ttgaagatcc cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc
    74041 tggtaaccag cgtcggttat gaaacgcctg gctctttcgg cagttggtgt gcagatctca
    74101 acttgcattg catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat
    74161 atctctttgc gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt
    74221 gaactgaagc gctggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa
    74281 actgacctgc ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca
    74341 gcccagcatc agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt
    74401 cagacccccg gttttatcga gcttaatatt aaccatctca tagcgccctt tcagcgcctt
    74461 caaattgcta cgagtatgac aactttcatc agcacaaatc ggcaacggat gaataaaatt
    74521 ctccagcgcc gcatcgtcct gcgcaggaag cggttgttca agcatcgcaa cgcctaaatc
    74581 cgccaatagc tggcaacgtg ccgccaaccc ttctgcacgc caggattcat ttgcatcaac
    74641 gatcagcgtg gcatcgggca cagctgtgcg aattgccacc atccgctcac tgataagatg
    74701 gttatccagc ttcactttca gtaatttcgc gccggcctgc cagagtgttg atgcgctatt
    74761 ggccatctga tcaggcgtac cgatgactac cgtctgtgca gtggtgactg tcccgggaag
    74821 cgttatgccg atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca gatcccacaa
    74881 tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg
    74941 tgtaagccct ttctctaatt gcggcacgac gctcataatt tgcgccatta ccgaggcatc
    75001 actttcccca taacgcggat acggcgtgca ttcgccggtg cctttaatgc cttcttcttc
    75061 cagttcaacc accaccacgt gcgcttcact gcgacttccc cgggcaatca caaacggggt
    75121 atgtaagggc caggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg
    75181 cggtggtctg cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac
    75241 taggtgtgac gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt
    75301 ccagggcaac ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac
    75361 ttttactctc gtggcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg
    75421 caaagtgctg aacattttcc cgagtattga taccggtgtt tgcgccgtat cagtacgtaa
    75481 gtttaaccaa ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt ctgccgatct
    75541 gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc
    75601 cactttccgt aacgctgaat tcctgcaagc ctacggtgtg gcaattgctg atggcccact
    75661 gaaaggtctg gcagcgcgtg ccgttgtggt tattgatgaa aatgacaatg tgattttcag
    75721 ccagctggtg gatgaaatca ccaccgagcc ggattacgaa gcagctctgg cggtactgaa
    75781 agcataagtt aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg
    75841 ttcttcttct ggcttaagcc atattcccgc aacttattgg caatcgcggt atgtgaaacg
    75901 ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc
    75961 gagcgttcaa aacggctggt gatttcatcc agcgaacctt ccatcgcatc ttcgcccacg
    76021 gctaccgttg cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg
    76081 tccagttgtg tcagtgcgcg atagatagcg ttctttaact gccgcacatt tcccggccac
    76141 gcataacgcg taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc
    76201 tcgtcggcaa agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca
    76261 cgtagcggcg gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac
    76321 acgccttttt gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg
    76381 acatgcacct catggtcttc gccaacccga cggaaagtgc catcattaag gaaacgcagt
    76441 aatttcgcct gcatccgtgg tgacatctca cctatttcat ccaacagcac cgaaccaccg
    76501 ttcgcctgct caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg
    76561 accgcatctt ctggtataga cgcgcaattc agcgccaggt aaggtttgcc cgctctgggg
    76621 cttgcctgat ggcaggcgta ggcaaaaaga tctttacctg tacctgtgtc acccgtaatc
    76681 agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc
    76741 gggctgacgg cgacaatttg actaaaggcg ctgacgtcct gggcggcaac attttgcaac
    76801 tgacggccca tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga
    76861 tcattttcat cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata
    76921 acaacatgct cgttatgcga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag
    76981 ccgttaatca attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc
    77041 tgacagctcg ccgggtttgc catatccact ttgcttttca tatcgacaga gagcacaggt
    77101 tcaggcaacg cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac
    77161 gggacagtac gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg
    77221 aaactctcaa actccagttc agcaaaattg aggtagattc gcccaatggg gtcaatctca
    77281 ataccgcgta aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg
    77341 agtcggtctt cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat
    77401 taatatgatg atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca
    77461 caaagacgga aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat
    77521 tatttttcgc tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga
    77581 cgacggaaat ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc
    77641 tccatagctt taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg
    77701 cgggtagaca aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc
    77761 agttcgctgg ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg
    77821 ctgtaatacc ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg
    77881 cgccaggcga taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt
    77941 gattccgccg ccgaacggct gatttcgcgc cgcgcctggg catctaaaac tggctggacc
    78001 aaatgcgcat acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac
    78061 cagcgttgca gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa
    78121 aatgcgcggc ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca
    78181 tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca
    78241 gcgccgataa tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc
    78301 caggcattca ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc
    78361 ccgcccatca ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc
    78421 gcttcaactt gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc
    78481 gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc
    78541 ggaccgtcga aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta
    78601 aaaattcaag cgcggcatca aggcgaatat gcggcagcgg tttgtcgaca tccatcacct
    78661 gcggacgaaa cgcctcaaac tggaagcctt gcttatccca gaacgcctga ccgggcaaac
    78721 gagcgggaac ttcgccagga taaacagtga gcggtgcgcc atcgctgagt cgattaccgc
    78781 gtagcgccgg gattttctca ccgttaacat caataatgcc gctggtggtc gcctgcaccg
    78841 aggccagccc caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct
    78901 gaatcagttg ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc acatggtccg
    78961 ctttagtggc agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg
    79021 tacgctgccc gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa
    79081 atgcctgtgg cccactgttg agaggttgca ggcaatcaac cagcacaatc tggcggtcaa
    79141 agcgcagaaa atgattctta tagaacccct tcaccacctt ctcgcagtaa taattaaacc
    79201 gctcgcgcag cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgcccc
    79261 aggcatcgac atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcccg
    79321 gcaagacaaa gcgcccaggc tgaataaagt gcagcccctg ctgtttacag tggtggagat
    79381 aatcggtcca cgcggcagca atgtctgcca gccggttttc gtcggcaggc gctagcgggt
    79441 ccagcccttc gcacatcatt cgccatttga ccgaccattc gccgcgctga ccattgagta
    79501 agcccgtcat ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca
    79561 accattcgcc gggataatcc acaatctcca gatacagcgt ggaggtgtcc ttgaagtggc
    79621 gtagcagcga atcgttcgat ttaaaacgta gcgccaggcg gatttcactg acgccgcgcg
    79681 ttggcgtcgg ccaggcggga ggatcgccat ataactgcgc cagtccttca tcatatgtga
    79741 agcgcggaat gccaaagtca cgctgaggaa tgcgttttac gcccagcagg cgctcttcac
    79801 gcaccgcgct taacagcggc aagcgtgctc cggcgtgaat attgagcaac tgattgacca
    79861 tcgcagtgat aaacgctgtt ttgccgctgc ggcttagtcc ggttacggcg aggcgcagat
    79921 gtctgtcgac accccgattc accagcgcat taagttcatt tttaagtcgc ttcattgctg
    79981 tcctgtgctg ctctgaagac cagaaaaaaa tcattcgtaa ggatatgaat gtgggggcca
    80041 ggccattgat tatcaactgc ccgtgttgtc atcgacgggc gaactccata agatgtgcag
    80101 cattcacgtt cggacggagt tcctgaccta tgtcccctac tatttatgat attgccaggg
    80161 ttgcaggcgt atcaaaatcc accgtatcac gcgtgctgaa taagcaaact aatatctccc
    80221 cggaagcgcg cgaaaaagtg ttacgggcca ttgaagaatt acagtatcag ccaaacaagc
    80281 tggcccgcgc gctgacctct tcgggttttg atgccattat ggtgatttct acccgttcga
    80341 ccaaaactac ggcgggtaat ccgtttttct ctgaagtttt gcatgccatc accgccaaag
    80401 ctgaagagga aggtttcgac gtgatattgc agacgtcgca caatccggca gaagacttac
    80461 aaaaatgtga aagcaaaatt aagcagaaaa tgattaaagg cattattatg ctaagttcgc
    80521 cagcggatga gtcatttttt gcccaacttg ataaatacga tattcctgtc gtggtgattg
    80581 gcaaagttga aggtcaatat gcccatgttt attctgtcga taccgataat tttggcgaca
    80641 gcattgcgtt gaccgatgca ttaattgaaa gtgggcatca aaatattgcc tgcctgcatg
    80701 ctccgcttga tgtccatgtt tcagtggatc gggtaaatgg ttataagcag agcctggctg
    80761 cgcataatat tgcagtgcgt gatgaatgga ttgttgacgg cggttatacc catgaaacag
    80821 cactgcaagc cgcgcgggaa ttattaagcc agtcaccgtt gcctgaggca gtgtttgcta
    80881 ctgacagcct gaaattaatg agtatttatc gtgcggcagc agagaaaaat attgctattc
    80941 cgcagcagtt agcggtggtg ggttatagta atgaaacgct gtcgtttatt ttaacgcctg
    81001 cgccaggcgg tattgatatc ccaacacagg agttaggtca acaaagctgt gagttattat
    81061 tccgcttaat ttctggaaaa ccgtcaccac agaatattac cgttgccacg catatgacgc
    81121 tgaaataaat accagctacc ttgcctgaat gccggatgcg atgctagcgc atcttatccg
    81181 gcctacattt atagttcgga taaagtactc gtgccgcatc cggcaattta gcccattatc
    81241 agaacgagta atttacgccg acacctgcat aatggaattt atcgctgtcg ccttcgtcgt
    81301 gatcctgcca ctcaaacgcg tattccagcg aaacggaaag cccgttctgg aaatcataac
    81361 cgtaaaataa acctacacgg ttaaaatcat ggccttcacg ttcaatatca tcctgccagt
    81421 cccagttact ccagcgatcc agcccaatgc gcgtatacgg cgtcaccgag tggttgccga
    81481 gcgtcagcgg caaataggcg cgaatttctt gcgtagaaaa ctcaccgtta ttgcggctgt
    81541 cgtccatatt aaagccgcgc tcgagataat agttcactcg caaggcaacc gtttcgttga
    81601 aggtatattg caggcctgtt tccgtttcga ctcgggtatc agcgtaaccg gtcgtgttca
    81661 gatcgttggc aaatttatac atcgacaacc aaccgttgaa acgtaaatcg tcagtcagtt
    81721 tcacatccca gtctggcgcg attttccagc gctgcatatt cgccgtgtct ttacccggtt
    81781 catcaacgta gtgataacca taattacgga aaccgccggt caggccgaaa ctgaaatcat
    81841 cgttttcgag gaactgataa tgcacctcca gctccgggcg atcaaaccac gttccacgtt
    81901 tacccgcgct ataatctacc ggcccttcct gataataggc cagagcaatt ctccacggcc
    81961 cgttggcggc attaaaatag actgaaggct ccgccagccc atccatatct tcgccataac
    82021 cctcgacgtt ttctatttcg tacatcgcgc cgatattaaa gtgccagtcg ttcctttcct
    82081 cggcctgtgc gcaggccatt cccgcacaca tcaccagtgc ggtacagggt aataactttt
    82141 tcattattgt tccttgtatt attctggggg aatgccttcc ccctgtattt agcgaattgc
    82201 tatttccgtt tctgcatcaa agaaatgaca tttcgtcata tcaaaatgaa tagtgatatt
    82261 ttctcctgca tgataatcat ttaacgcacc agcacggacc actaactcgt gccccccaac
    82321 cgtggtgtag agcataaatt ccgcaccggt taattctgcc acgctaattt tggcggaaat
    82381 gttattttcc tcttgcgcgt ccggatgaat atcttccgga cgtattccca tcacgatggg
    82441 cttatgcaaa cttccctgtg ttttcagaac cgctaatttc tcttcgggaa tggttaattt
    82501 aagcgtttcc gtaacgaatt tatcgccatc gatcgtgcca cgaataaaat tcatcgccgg
    82561 tgatccaata aatccggcaa caaacatatt cgctggttgg ttataaaccg ttttcggcgc
    82621 accaacttgc tgaacaatcc catccttcat aatcacaatc cgcgtcgcca tggtcatcgc
    82681 ttcggtctga tcgtgggtca cgtagatcat ggtggtattc agcttctggt gtagcttgct
    82741 gatctctgcg cgcatttgca cgcgcagctt ggcatcaagg ttagagagcg gttcatccat
    82801 taaaaacacg cccgcttcgc gcacaatcgc ccgcccgagc gccacacgct ggcgttgccc
    82861 gccggaaagc gcgcccggct tgcgtttcag gtactcacgc aggccgagaa tttgcgccgc
    82921 ccagttcacc cgctcatcaa tcacctcttt ggcgattttt tgcatcttca ggccaaacgc
    82981 catgttgtcg taaaccgtca tatgcggata caacgcgtag ttctggaaca ccatcgctat
    83041 attgcgtgct ttggctggaa cgtcattcat tcgtttgccg tcgatcaaca gatcgccgcc
    83101 gctgatctcc tcaagcccgg caatcatgcg cagggtggtc gacttaccgc agcccgacgg
    83161 gccgacaaac acgatgaact ctttatcggc aatttccagg ttgaagtcct tcaccacatg
    83221 cacctggtta tcgtagattt tttgaatatg ttgtaacgaa agctgagcca tgttgattcc
    83281 tttgctatac gttttgccag aaggccgata accgcgacca ggtcagagag tccgttgaag
    83341 gcaacagtaa ttgcgccccg gttaagcccg cgccgatccc caccgagcgc ataccgctgg
    83401 cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc gatacatgcc tgcggcggca
    83461 cgcccagccc ggcacaggcg gcgagaaaga tttccgggtc cggtttcgag tttttaagtt
    83521 gggaagcatc cgcgcagaag gtgaaaaact cgcgcagctc cagcgccgct aaaatcgtcg
    83581 gcgcattcag ggagacagaa gccagcccaa ccgggatctg ctgtgcacgg agatctgcca
    83641 gcaaagagcg aatgccgggt agaacagcgc tgaccgtcaa ctcgcgcagt gagtggacat
    83701 agagcagatt tttgcgatac gccagttgcg ccctctcctg cgagttaaag tcgccctctt
    83761 tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg gctgatccct tttagggatt
    83821 cgttaaactg cgcatcaatg ctgatgccaa tttcagcggc aatctgctgc cacgcctgga
    83881 aatgcagatg cgcggtatcg gtgattacac catccagatc gaaaattacc ccttgcagtt
    83941 tcattcatcc tcctgatgtg tggtagcggt cccattgaag ggcaaaataa aatcacccaa
    84001 acagaaaaca gattcttcag ccacggatat aagctgaccg ttcaaacgca gtgaaacggg
    84061 cgcagaagtt cgaatcgcaa tacgctgcgc gtcaagagtg acctgtaatt cgcagccctg
    84121 ccagaacaga gggaaagaca actgttgcca ctgctcaggt aacgccggat tgagatgcaa
    84181 ttcaccgtca cgcacgctca ccccggcaaa accctgaatc gcccccagcc agatagcgcc
    84241 agttgcggca gcatggatac catcatcaca actatgcgga tcagcaccaa gatcgatttc
    84301 agtcccctcg cgccagaact gataactttg ggtcagcagg ccacagcgtg cggcaacaat
    84361 gccgtggatt gctttactta atgacgagtc gtgaatagtg cgcggttcat aaaattgcag
    84421 attggcaaga cacgatgccg ctgagaactg ctccggcagc atgtaattga gcatcaccac
    84481 atcagcttgt ttgaggattt gcatctcgtt cacttctgcg cgtgaataat ccagcagaat
    84541 ggtttgcttc cccgccgccg ctttgtattt cgccagatta atcgccggct tagccataaa
    84601 cgaatcatcc tgcggcaaaa cgccgtcggg ctgaatttct ggcagccgca gttctttaag
    84661 gaacatttcg gcgcgatgga taaacgcatc gtcgctacag ccaaactggc gggcaatact
    84721 cagcgcctgc tggacgttgt aatacgccat atagctggtg aaggcgttat tattgacatg
    84781 ttcggtatat tcgtcaggcc caataacatc atgaatttcc agacggtcgt taaccctcac
    84841 cgcgcggcta atccagaact ttgcagtttc cagaagtagc gccatgcctt catgcgcaat
    84901 gaaactttca tcccccgtgg tctgccagta ttgaataacc gcccaggcga tatcggccac
    84961 cagatgatgt tccgcctgcg ccgaggccac tttttgccgc agcccggtgc gaatgttaat
    85021 ggcggcaaat tccggcgtct cttcttcgcc gctgcgcgcg ctttcccacg gaaatagcgc
    85081 gccctgccag ccgttgcgtc gcgctttctc ctgcgcgcct ggcaagttgt gccagcgata
    85141 acgcagtaaa cttcgggcaa ccgtcggatc gctaaacaga tgaaacggta acaaaaatac
    85201 ttctgtatcc cagaaaacgt ggcctttgta gccttcgccg gttaagcctt ttgccgcaat
    85261 gctgctgcgc tcgtcgtggg caggcgtcat gatgcgcaga tgataaagcg cataatctaa
    85321 cgcttgctga tcgtgcgctt cgccgccatt taccgtgata cgacgtttct gccaccattg
    85381 acgccagttt tctgttgata ctgcaagaag ttggtcgtaa ctctgctgcg cgcacatttc
    85441 aagctggcga agcgacgcgc tgccccactc gtctaaaacg gcttgcctgt catcccgcca
    85501 gtcgatccac accagttttt gcaacgtcac tgtctcgcct gcatgaagct gcgcactggt
    85561 atgttgctgc aaacggcgct ctttggcggt atagcattgc tgcacatcac cgctcaccat
    85621 acagcaacag ctgatggcca catcactgcg tccatcctgg gtggtgtaga tcccctgcat
    85681 cagatgctga ccaaacaccc gcacctgggt ttcgtcgaga tgttggcgac cgtggttggt
    85741 ttgcgtggcg tcgatacctg ttgaaatcag cactgacgcg tcggcgtcca gtggcgtaat
    85801 agtgatttcc agcacaatga gcggcagttg gtctgccgaa acaaagcgac ggctggcgat
    85861 ggtgtaacct gtgccgttgc tggtacgcca gacaacgcta cggcgtaatt ctccactggc
    85921 aaagtcaagt tcacgctgcc aggcttcgcg ggataacgag aaaacctcac catttatggc
    85981 aatctccatc cccaagatat caggcaggtt caccagttcg ttgatttcac cttttcccgc
    86041 ccgatgatac agccccgcca gatacatccc tcgcgtctgg cgcgtgtaat cttcttcatg
    86101 gctggcgcga agcccaagat agccgttccc ctgcgccatc agcgatgcat acttgttcag
    86161 ggtatgctgg ctgaaatggg gttctgataa cgttactggc ctggtcataa ttccacacgc
    86221 gtccctgttt ctgcggattg atacaacgcc gccaccagtt gctggatgat gtacccctgc
    86281 tcggcgtcgg ctatcatcac gggcttgccc tgtacgtgat tgataaaggc ttccatgctg
    86341 cgcaaatggc ggttgtcgtc tgccagttcc cgttgcatca gcgtcattaa ttcaccgttg
    86401 ttatcggtgt agatatgtgc tggaaacagc gtcgcaccgg ctttatcacc acaaaagctg
    86461 acgttcataa tcgactgttc gcggatgttg agtgcaaatg acgtttccag ccacagaatg
    86521 ccgccgttat gaaattcaat ggtgccaaat aacgaatctt cgacgctgta agttgccgga
    86581 tcccactcgc caaattgacc gcagctcttt tgcgtgccga tcttttgaaa gctatgcgca
    86641 ttcacgcttt tcaccgccgg aaagcccagc acatacatcg ccgcatccag catatgaatg
    86701 ccgatgtcga taagcgggcc accaccctgc agttctttat tggtaaagac accccagccg
    86761 ggaacgccgc agcgacgcag ggcgcgggcg gtggtaacgt aaatctctcc caaaacgccg
    86821 ttggtcacct gttcacgcag ctgttgcgta tcgagcgcaa aacgatggtg aaagtcgtag
    86881 gccagcacct tgccctgttt gcgcgcggta tcgcacattt cccgcgcctg ttctggcgtc
    86941 atggcgggcg gtttttcgca catcacatgg cagcccgctt ccagtgccat cagggtatgt
    87001 tcgtaatgaa aacggttagg tgagcagacg ctaaccacat caggtttcac cgccagcagc
    87061 atggcctgcg gatcgtccca cacggatgca ttaccgtatt tatctgccag cgcctgcgcc
    87121 tgggaaaggc ggctatcaca gacagccacc agttccagat cgttgcgggt gcagtagtac
    87181 gaagcatgaa ccttatccgc cacctggcct gcgcctatta tcgcgacccg cagcggagag
    87241 cttgtcattg cacttttcac aaagacctct tagcaggtac gcaaccaggc caacgaatca
    87301 cggtacgcct gggcaggatc ttctgcccgg atacgctctt catacaccac ataaccctga
    87361 tagttatcgg cgcgcagctg ttcaaacagc gcgtggaaat cgagggtgcc actgcccggc
    87421 tggtaacgat ggttatcggc aatatgcaca tgaccgagca ggtcacggtt gtcgtgcagc
    87481 gcctgcgcca ggttatcttc ttcgatgttc atgtgataga aatcgccgat aatctgcaca
    87541 tgtttaagat tgttttcgac gatgtaacga cgagcatcgg cgagggtgtt gatcatatga
    87601 tcctgatagc ggtttaacgg ttcgagatac accacggttc cggtacgcgc ggcgacctgt
    87661 tccagtacgc gcagggaatc actcaccatt ttgcggtcgc cgtccaggct acgcggcgag
    87721 gtcatcggcg gtaagcggaa ggtaaacatg ccccacgctg ccggaacgac gatgccttta
    87781 ccccccactt ctgccagcgc ttcgagaatg cgttcgatct gctttaagcc attaagacga
    87841 cgctcttcga taaagtcacc aatccagccg tcatagccac cgcaggtggt ggtcaccggt
    87901 aaaccagttt ctttgatcgc cgctttgact tcttcgagat tgttgaccag cagtttgccg
    87961 tcgatctcaa aaccatcgaa acccatctct ttgatataac gaaatttctc cagaatattt
    88021 tccggaaaga acgcctgatt ctgtgtgcct attttcattt ttttcattcc tttgaattaa
    88081 aacgtaacgc ccattttgat gctctgttcc ggatgctggt cgacatactg catgtagctt
    88141 tccgggctat tggcaaaggt caccaccggg tcgattagat cttcacaatt gagataaccg
    88201 ttcatcagca gttcccaaca ggtttcttcg atacgcttgc ggctccagcg cggataatcc
    88261 gggttcggtt cgctgcacgc gcgggagaag acaattttgg cgttattgaa atgggcttcg
    88321 cgtccgaggt taaaaccttc ggcaaacggt ttggcaaacg caacatacga gatggtgccg
    88381 ccataagcca ggccgcggag cgccgattgc agcgcgtcgg cgtaaccgct ggtttcgata
    88441 atcacatcgg caccctgctt gccggtcagc gttttgatct ctttacctac gtcagtgccg
    88501 atggggttaa ggcagaaatc cgcaccgtgg cgacgggcaa tatcacagcg atgggcgata
    88561 ggatcgacgc caattaccac cgatgcgcca gcgcgtttcg ccagttggat ggctatttga
    88621 ccaatcgcgc caagccctac caccaccaca aaatcaccca cgcgcacgtt ggcatcgcgc
    88681 acgccgctca tggcaaactg cgccgggtcg taacagacgg cgtttttcca ggagctgcct
    88741 tgcggcattt tgcgcagctt gtagttattc accgcgttaa tgatgaccgt ctcggagagt
    88801 gggccgtagc cgcatacgct gtcgcccacc gcgtagtcgg ttacgtcgct gccgcactcg
    88861 ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat gccgcgcggc
    88921 gcatctgccg gacgcggagt gaacatctgc cattcaccgt taaagtcttc atcgataaac
    88981 gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc gaaacgcacg
    89041 cggattttca cttcattggc taaaatcgcc cgatcttcat actcaaccag cgcagcaaca
    89101 cgcggtgctg tggctactaa ctttttcatt taaaactcct tgtaaagaaa ctggccgctt
    89161 tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccga aagcgcatac
    89221 ataatcacca ccggcaatgc ggtcaccagt gaggccgcca tcatccgtcc ccagatgtaa
    89281 tctggcgtac tgaacagcgc gttcaggccc accggtaagg tgaaattgct ggcgctggag
    89341 aggaaaatcg acgcaaacag atagtcgttc cacgccacca taaagcagta gacgaacacg
    89401 gatatcagcc cggacatcgc caacggcacg gtgatgcgga agataatttg cagacggttg
    89461 aggccatcca tcatcgccgc ttcttcgatc tcatcgggga tggtgtcgaa gtagcttttc
    89521 agcataaaca cggcggtggg cagagtttgc gtcaccatgg tgatgatcag cgccatctcg
    89581 gtgtcgtaaa tgcccagcgc ggtgatgatt ttgaacagcg gcaccaccag caaaatgcca
    89641 gagaacatat acaccgtgta aaagctggca ttgatggtca tccgaccttt aaagcgcagg
    89701 cgggaaagcg cgtaagcccc aagaatgccg aggaataccg ccaccacgga tgaaaccacc
    89761 gacaccacca gactgttacg gaagtagtcg acaaacggga aaatcatcgg gttaaaaatg
    89821 tcgacgtaat gctccagcgt ccactgctgc ggcagcagcg taggatgcag tgagatcgcc
    89881 tctttcgcgc ccttgaacga ggtcatcagc atcacaaaaa atggaaacag cgtgatgatg
    89941 agaaacagcg ccagcccgca gtaaaaaccg atgcgactga gtgtgcgttt atttgttgcc
    90001 attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga tgaagagcac
    90061 caccgagatc gccgccgctt ttcctaaatc attaaaggca aaggcggttt tgtagagata
    90121 tacaccgaga atatcgacct tggtcgtcag caaataaaca tccgcgaaca tgtagaacat
    90181 ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataatcgcgg gcagcgtgac
    90241 gatacgaaac cgttgccagg cattagcgcc atccatctct gccgcttcgt acagcgattt
    90301 gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt agcgccagat
    90361 ggcgaacagc accaccagca caaagctact gcccggatta tcgaaccaca gcggtgcctg
    90421 ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc cgttgttgaa
    90481 catgtatttc caggcaaaca ccagcgaaat ggacggcgtt acgtaggaga ggatcaccag
    90541 cgaacgcgcg gttttgcgca gacggaactc gcggttgaaa aacatcgcca ctgccagccc
    90601 gagcacggtg ctgcccgcca ccaccagcgc ggtataccag accgtcatcc acagcgagtg
    90661 ccagaagccg ggatcggaga ggatacgcac atagttactc accccaacaa aggttgactc
    90721 gatgttggga ttgagcggca gacgtaaaaa actgatttcg atattcgaca ccatcggcca
    90781 cgccaccaga ccgcccagca gtaataagct gggcgcgaga agcaacagcg caaagggcat
    90841 atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtccggggt ttagtgctgt
    90901 tcgatcagtt catccagctt tttctggctc gcttgcagcg tgccagggag atcggcttta
    90961 cccacggtga cgttatgcac catggagctc accactccag accccgtcac atcacccatg
    91021 cgggtaaagt ttttatcccc tactgcacca aagacctgaa tatttggcag ttcaccgatt
    91081 agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca ggtggcggta
    91141 gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac ccagtcggca
    91201 atgttgtctg cctgctccat aaaggtgaca aatttttctg ctgcttccgt ctcttcggtc
    91261 ttttgcccgg cggtaatggt cagcgaggtc aacatgccgt agaccgcaga gtttttctcg
    91321 gttggcacca cgaaaccgac gtttttcggg tcgccttctt taatcacagc cggaaggata
    91381 taggtggagt aaatcgccat cggcgcggtg ccgttcataa aggcgtcttt cacttccatg
    91441 atgtcgttag aacccggcat agtgttggca gcaaggtcgc ggtaataggt cagtgcctgc
    91501 atcatctctg gtgtatcaag ggtgattttg ccttcggcgt taaagacgtt agcctggttg
    91561 gataacgcaa actgggagaa ggattgttcc gtcaacacgc tttctgctgt aggcagcgca
    91621 atgccgtatt ttttattcgc cgggtcattc agtttctgtg caacgtccag cagctgttgc
    91681 cagtttttcg gctcctcaag ccccgctttt gccagcacat ctttgcgata ccagataccg
    91741 ccaatccagg cgctgacagg aacaccggtc catgcgctac catcttcggt acgcacaata
    91801 cgcagtacgc catcgtaaaa cgcgccttca ccaacgttgc tgatgactgt ggcaaccgct
    91861 ttgcgatcga taagctgctc tttgtccatc actttggcgt agtcatggct ggtttcgatc
    91921 acttccggca gcgaaccgct acgtgaaaga gtaatgactt tagtgttata ggcatcttct
    91981 tccacgggca cttgcttaac ggtgatgcca gggttttctt tttcaaaacg ggcaatcaat
    92041 ttactgataa cggcctggcg ctcctgctcc accgaagaat gcataaattc gatggataca
    92101 ttcgttttat tttcttcttt acagcctgaa atcagggcgc aggaaaccag tgctgataac
    92161 agcacgattt ttgatttaat catgagatgt tcctttttat ttaatccaca taacctgcca
    92221 ggggcgcagc gttatttcat tacccaatat atcaacttca ctaatcagat cccgaccatg
    92281 cagattagta atatttacat gctgaatatt tttactgaca ttaaacaatc cagtcagaca
    92341 attaccatca gcgttacttc tttgaatacg cattacggat gaattaatcg tatcaatggt
    92401 aaaattatta tccggatgaa actcgttatg gctgcgacga agtgtaatta aacgcgacaa
    92461 ctcatgatat accgcatgcc ttaatgtagc ttcatcgttc agttctcggg ttatctcttt
    92521 actgtgatat tttttacggt taatcgcacg gttatatccg agtttttcga cacctgcgta
    92581 atcattacgc gagccaagaa tactttgaat atatatcgct ggcacaccgg ggaaacttaa
    92641 caaaatcgca tgggcgagaa taaacctggc gcaacgttct tcatcgctac tctcacggcg
    92701 gcttaacgca tccatatagg tcacatttat ttcatacgga ctgcgtgtac cgtcgggatt
    92761 atttttccag tttactaatg caccttcctg ctgtaacgcc tcgaccagct ctaatatttc
    92821 gctttcaggc aacaagcccc gtagcgggtt tagcccgatg ccatcgtgag aggcgaggaa
    92881 gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt tgcgcccacg cacaaagcgc
    92941 ctcaacgttc tgtttttgca ccgcatgcag caccagtggc ggcagcgaga actggtacac
    93001 catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg ttgtctttat gcggaacatt
    93061 ggtctcggta atgatcactg tacctggcgc aacgttatca ataatcgacc gtaacagttt
    93121 gataatcaga tgtgtttttt ccagatggat gcagcttgtt cccggctctt tccacataaa
    93181 gccaacggca tccaggcgga catactcagc acctttcgca aggtaacaga gtaaaacatc
    93241 caccatcgcc agcaacactt cagggctacg gtaattcagg tcaatttgat cgtcactaaa
    93301 ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg aatggcgtta ataacggtaa
    93361 cgcacgcggg cgagtgacgg cgctgagatc ggtttgcggg tcaacggcaa taaaaaaatc
    93421 ttcaaaacct ggatgctgtt gtaaatagtt tttaaaccat tcactttttg ccgacatatg
    93481 gttgcagaca aaatcaaaca ttaaatgact acattcaccg agttgctgaa tatcctgcca
    93541 ctcccccgct tcactggcga cctgttgata atcaattacc gaaaagccat catcagatga
    93601 ccatggataa aacggcaaca aatgaacatg tgaaaaaatg ctttgcagcc attgatggta
    93661 aaactgatta aatgtgggta atggttttaa atcattgctg tgaaattgat cggcataggt
    93721 aattaacacg acatcacttt catcccagtg ttttttacgt cgctgtgtaa ttaatcgttt
    93781 cgcactctca agacgcgtta caagtttctg taaatgagtt gcggtaaatg ttccaccgta
    93841 gatttcgtcc aggtaatccg taattttctg tttcatgttg cctttccgtg gtagcggtcc
    93901 catgaaacga gagactactc cgcagaaaat aaactgtcaa ttgagcacaa gggttttagt
    93961 cacgcaggaa atcatttttt accagggaaa aagcgtattt gtgagctgac gcaaataatc
    94021 tatggggatg taaataaggt gtgacagaaa aaaaacggcg cataagcgcc gctcatggtg
    94081 aattctttta acctttgacc ttcggcattg cgatgtcttt caggccaccg gcgttctcaa
    94141 cgtgggtata tcccatctcg ctaaggatct cttttgcttg ccctgactgg cgtccggcat
    94201 tgcaatacac tttcacggtg tcgtttttat ccggaacggc ggtggcaatg cgctctttca
    94261 cttccttcag gggaatattg atggccccct gaacgtgctc ttgctgatac tgctctggaa
    94321 cacgaacatc gatccagtgt tcagcggcga aaacgggcag tgaaaacacc agtgccagag
    94381 caagtaagcc ttttttaaac atgtaagcgt ccttctatgg atgaaaattg ccatccatta
    94441 tacgcctgag atttaacaat ttttgcgaaa ctcccctcac cttttataac gctgtgccag
    94501 tttattagca gcccgactta acagcggttc cagcgccaca gccagcaaca ttttcagcgg
    94561 tcggcgagca actgatttta tcgcccagcc cgccacaccc gccgggccat agcgcagtgc
    94621 ggtaagaagt accagcttgc ctgctaattt gaaaccaggc tttacctttt gcccggcctg
    94681 ttgccagcga gtattcataa ctttcctcac agttgacgga aacggctacg taacgtgaaa
    94741 gtatcggaag tgacataacg ttccatctcg cgtaaacgcg tttcacttgc cgccagttca
    94801 cggtcgactt catccagcaa ttcgctgctg gaaggtagct gctcaccaaa ggccatgttg
    94861 tccggcattg gatcaagcgc aaatgacaaa atgatgtaag caaccagggt aaacagcgcc
    94921 agaccgaaga aaatcgacag caccaccagg atacgcacca gttttaccgg tacatcaaaa
    94981 tagttggcaa tcccggcgca gacgccgcgg accatgccct gctgtggaat acgccataat
    95041 tttttattga gattaatgcc cgccattagc gatccctcca gttcggatgt tctgcatcaa
    95101 gaatagattc cagcgcctga atacgttcgc gcatccgttt tgcttcatca gccagttgcg
    95161 ctaatcgctg ctgctcactt tgcgacaatt cactgcgacc agaacgattg ctgtaatgca
    95221 gccataacca gatcggtaaa acaaacagca caaaaatggt taacggaata gccagaaata
    95281 gcgcgctcat aagtactcct tagacgatga gcggcggcgc gttcggacgc cgcctggatg
    95341 ttattattga ttgtcttgct tcattttggc ttttaattgt gccagttgtt cgctgattgc
    95401 atcatcggct ttcagttcgg caaactgatc gtccagcgat ttttgtttac cgaagctgtg
    95461 gctttctgct tccgcttcca tctggtcaat acgacgttcg aaagattcga aacgagccat
    95521 tgcttcatcc agtttgccac tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc
    95581 ctgatgacgt aacatcaatg cctgctggcg agcgcgtgtt tcgctcaatt tgttttccag
    95641 ctcaccgatc tctttcttca tgcgtgccag cgtgtcgtcc accagcgtca cttcatgttc
    95701 caggctctta atcagatcgg tcagtttctg tttttcaatt aacgctgcac gtgccaaatc
    95761 ctctctctct ttcagcagcg ccagttcggc tttttcctgc cattcaactt cacgcgccga
    95821 cgcttgttca atacggcgag tcagctgttt cttttctgcc aacgcacgcg ccgaagtaga
    95881 acgtacttca accagtgtat cttccatctc ctggatcatc agacgaacca gtttctgtgg
    95941 atcttccgct ttctctaaca gagcgttgat gttggcattc acgatgtcgg caaagcgaga
    96001 aaaaataccc ataatgttgt cctcttgatt tctgcgttca ggattgtcct gctgaactgt
    96061 taatacaatt tgcgtgccaa ttttttatct ttttgattta taaagatctg attgaagaat
    96121 caacagcaac atgccaggat gagttagcga attacactaa caagtggcga atttcatcat
    96181 ggcagaatac aaagataatt tacttggtga ggcgaacagc tttctcgaag tgctggaaca
    96241 ggtttcgcat ctcgcaccgc tggacaaacc ggtgctcatc atcggcgaac gcggcaccgg
    96301 taaagagctg attgccagcc gcctgcatta tctctcctcc cgttggcaag ggccgtttat
    96361 ttcccttaac tgcgcggcgt taaatgaaaa tctgctggat tccgaactgt ttggtcacga
    96421 agcgggggcg tttaccggtg cgcaaaaacg tcatcctggg agatttgaac gtgccgacgg
    96481 cggtacgcta tttcttgatg aactcgctac ggcaccgatg atggtgcagg agaaattatt
    96541 gcgcgtgatt gagtacggtg aactggagcg cgttggcggc agccagccat tgcaggtgaa
    96601 tgtgcggttg gtatgcgcga cgaatgccga tctcccggcg atggtcaatg aaggcacttt
    96661 tcgcgctgac ctgctcgacc gactggcttt tgatgttgta caactgccac cactgcgcga
    96721 gcgcgaaagc gacataatgt tgatggcaga acactttgcc atccagatgt gtcgggaaat
    96781 caagctgcct ctgttcccgg ggtttacgga gcgcgccaaa gaaacattgc tgaattatcg
    96841 ttggccggga aatattcgtg aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg
    96901 caccagcgat tatccgcttg atgacatcat tattgatccc tttaaacggc gtccgcctga
    96961 agacactatc gccgtttcag aaaccacctc gcttccaaca ctgccgctgg atttacgtga
    97021 gtttcagatg cagcaggaaa aagagttgct gcaactcagt ttgcaacaag ggaaatataa
    97081 ccagaagcgc gcggctgaat tactggggtt aacttatcat cagttccgcg cgttgttgaa
    97141 aaagcaccag atttagcgaa gttgagcaat acaacttgcc ggatgcggcg tgaatgcttt
    97201 attcggcata cataatcaat acgtcgtttg caggcctgat aagcgcagcg catcagacat
    97261 tattgcgttg ggctattaat cgctcagcgc atcctgcaaa attttcattg ccgcatcgaa
    97321 ttgcgcatcc gggatggtca gcggatacag gaagcgaatc acgttgccgt atgcgccaca
    97381 ggtcagcagg agcagcccct gcgccagcgc gcgttgctgg attttctgtg caatcgccgc
    97441 tgacggctcg cccgtttgcg gatcgttaaa ctctgccgca atcatcgacc ccaggccgcg
    97501 taccgcagca atggccggaa cgctttcttt ggcatcaatc aacgtgtttt tgagacgctg
    97561 gcccagttga ttcgcgcgtt cgcagagtga ttctttgtcg ataatattga gcaccgcgtg
    97621 cgcggcagcc accgccagcg ggttcccggc gtaggtgcca cccaacccgc ccggcgcggg
    97681 cgcgtccata atattcgcgt taccgaccac gcccgaaagc ggcatcccgc ccgcgaggct
    97741 tttcgccatc gtcattaagt ccggcttatc ggcgtaatga tccatggcaa acagtttacc
    97801 ggtacgcgca aagccgcttt gcacttcatc ggcaatcatc acaataccgt gctcgtcgca
    97861 caggcggcga atggcggcaa ccagctcttt tggcgcaacg ttgaaaccgc cctcgccctg
    97921 caccggttcg aaaataatgg ccgcgacctg cttcgcttcg atgtctgatt taaacaagcg
    97981 ttcgatggcg tcgagggagt cctgtgttga aatgccgtgt aaatctgacg gataaggtac
    98041 gtgatacacc gaaccaggga acgggccgaa gccgattttg tacggcgcaa cttttccggt
    98101 cagcgccatg gtcatatatg tacgaccgtg gaagccaccg ctaaacgcaa tcacgccagg
    98161 tcgtccggta tgggcgcgag caattttcac cgcgttttcc accgcttccg caccggtagt
    98221 gaagaatgcg gttttagcct gcccgctcac tggggcaagg gcgttaattt tctccgccag
    98281 cgtgacgtag ctttcgtacg gcacaatctg atacgcggtg tgggtaaact gttgcaattg
    98341 ctgctccacc gccgcgacca gatcagggtg gcgatgcccg gtattcagca ccgcaatgcc
    98401 tgcggcgaaa tcgatgtatt cgttgccctc tacgtccttc agcgtagcgt tttctgccga
    98461 ctgggcgaag aagttacaca tcacgccaac cccgcgtggg gtagcagaaa gacgacgctg
    98521 atggaattca ttgttgctca tgtttcacac cttttgactc ttcactgtta atcggaaatt
    98581 aaacgcccag acgatcgcgc aggctgtaat aagccgcgcc catcgcggta aatggcacgc
    98641 gcagcgtgcg cccgccgggg aacgggtaat gcggcagatt ggcgaaggca tcgaaacgtt
    98701 cggcgtcgcc gcgcagcagt tcggcaatca gacgcccggc aagatgggta caggtgacgc
    98761 cgtggccgct gtagccctgc atgtaataga tgttggtatc aaggcgaccg aactgcggca
    98821 tgcgcgacaa ggtcagcagg aagttgcccg tccagcggta atcaattttc acgcccttca
    98881 gctgcgggaa ggttttcagc agtttcggca ccacaaggcg ctcaacgtca tccgggtcgc
    98941 gcgcgccgta gaccacgccg ccgccgtaca gcaggcggtt gtcggcggta agacggtagt
    99001 aatcaagcag atagttacag tcttccacac agtagttttt cgggatcagc gaacgggcta
    99061 aatcttccga cagccgttcg gtggtgatca cctgcgtgcc gcacggcatg ctgcgtttcg
    99121 ccagttccgg ctctacttta tcgcccagat acgcattccc ggcgacaatc acatacttcg
    99181 ccgtcacctg acctttggca gttcgcacaa cggctggcgt ggtgtgctgg atctgcgtca
    99241 cggcagaaag ttcatacacg cgcccgccgt tgaggcggat ggcgtccgct tcgccaatgg
    99301 cgaggtttag cggatgaata tgcccaccgc tgtgatccag cagcgcaccg gtatagcgat
    99361 cgctggctac ttcacggcga atggcgttgg cgtccagcaa ttccagctgt ttattgccgt
    99421 agcgttccca gttctctttc tgctcttcaa gtgtggcgag ctgtttatca ttcatcgcca
    99481 caaacaggcc gccggggcgg tagtcgcagt caatctgata acgtttgata cgttcgcgga
    99541 tgatctcacc accttcgaac atcatgctgc cgagcatacg ggcggtgtcc atgccgtagc
    99601 ttttttcgat cacgtcgatg tcgcggctgt aggagttcac aagctgcccg ccattgcgcc
    99661 cgcttgcgcc aaagccgatg cgtgaggctt cgagaaccac tacgtcaaag cccgcttccg
    99721 ccagatgcag cgcggaagag agcccggtat agccgccgcc gaccacgcaa acgtcgcagg
    99781 aaatcgactc attcagcgtg tcgaatggcg cgtatttatt cgcactggcg gcgtagtaac
    99841 tgctggtatg ttcggtcatt tcaggcctcc aggcttatcc agatggtttt cagttcggtg
    99901 aatttttcaa gggcatgcag ggatttgtcg cgaccgttgc cgctctgctt atagccgcca
    99961 aacggcacgg tcatatcgcc gtcgttgtag ttattgacga agacggaacc agccttcagg
   100021 cggcggctca tgcggtgcgc gcgggagagg tcgcgcgtcc ataccgccgc gccaaggccg
   100081 tactggctgt cgttggcaag ctgtagcgcc tggtcttctg atgtgaaacg cgtgaccacc
   100141 agcaccggac cgaaaatctc ttcgcggctt aaggacgcat tcgggtctac atccacaaag
   100201 atggtcgggc cgatggcggc agccagctca gcgttacgac catccagcag cagttgccct
   100261 ttgctttcgc cttcccgaat aaagctatgg accgagtcgg cgtgggcgca gtcgattaag
   100321 gtgcccatgg tggttgcggg atcaagtgga tggcccggct gccagttttg cgcctgctgt
   100381 tttaacaggg ctaagaattc atcggcgatg ctctcttcca gcaacaaacg cgttccggcg
   100441 atgcacacct ggccctggtt gtagaagatg cctgcggcgg tggcgcttgc cgctttttgc
   100501 agatccggac aatcggcgaa aacgaggttg gcgcttttgc cgcccgcttc cagccagacg
   100561 cgtttcatgt tgctgtcgcc cgcatctttc agcagctgtt tcccggtacg ggttgaaccg
   100621 gtaaaggcaa tggcgtcgat atcgttatga cgcgacagcg cctgccccgc ttcatgacca
   100681 aaacccgtca ccacgttcaa cacaccatcc ggcaagcctg cttctttcgc cagccccgcg
   100741 agacgaatcg cactgagcgg tgatttttca gacggtttta gaatcacgct gtttcccgcc
   100801 gccagcgccg ggccgagttt ccagcaagtc agcaacagcg ggaagttcca cggcacgatg
   100861 gcggcaatca cgccgaccgg ttcacgcacg atcatcgcca gctcatggct actggtggtc
   100921 gccacttcgc catacacttt gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg
   100981 ccgggaatat catcacgcag actgtgacga atcggtttgc cggtgtcgag agtttccagc
   101041 agtgccagct cttcggcgtg ggcttccatt aaatcggcga gtttattcag taccgcttta
   101101 cgtttagccg gagaagagag tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc
   101161 accgcacggt cgatatcgac gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg
   101221 accggatcaa cggtttcaaa ggtttcattt tccgccgcag cagtatattc accgttaata
   101281 aataagcggt tttcaatggc gagacttaac gctttatcct gccagtaagc cagatgatga
   101341 aaattcatta tgactcctgt ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg
   101401 cgcagagatc cccgggccgt atgcccgttt atttctggcg acattcttta tatacacttc
   101461 atccttcaag ccgcctctgc cttggctgcg ttcgctcccc cgaatcactt acctgagtaa
   101521 gctcatcggg attcgctcac ttgccgcctt gatgcaactt gaatgatttt gtgtatagat
   101581 tgccgggcgc attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt
   101641 atgggcgctg ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcggtatgcc
   101701 ggtattaatg gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag
   101761 aacaatttca ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc
   101821 agtggttgtg cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc
   101881 gttatgaacc agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt
   101941 aatgacgacc tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag
   102001 accatacacc ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc
   102061 tatcgtactg atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag
   102121 cccctgctgc tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat
   102181 atttgcattt ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga
   102241 caagcggtga taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat
   102301 tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact
   102361 aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgc
   102421 ccatgcagag agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc
   102481 ccctcttcaa cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga
   102541 tcttcccgat gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg
   102601 gttgccacca ccagttcctg taaaccccgg cagatggcga aaatggggat gcgcctttcg
   102661 agtgcggcat taattaacgc catgctcaga agatcacgcc cgggatcggc gtcaggctca
   102721 tcgccgtttt caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg
   102781 ccatcgagtt tcggcaaaag ttgttcaagt aatgacggtt ccgccagcgc atgtggtagc
   102841 gcaattggca agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg
   102901 gtcgcatgac ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt
   102961 atattttcca tgctcaatct cacaaagtgg actaaattat cgccattact gcggtttaaa
   103021 tgacctttat gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa
   103081 tttaacattt gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta
   103141 cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt
   103201 gaagtagaga actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa
   103261 gtgaaacgct acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat
   103321 ttgaatggtt gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gttggagaaa
   103381 gggtgttatt tcccggcctc ggtgtttgca atggatattc tgggcaacgt agtagaagaa
   103441 gcgggtctgg gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt tctcggttcc
   103501 ttaacccctt ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat
   103561 gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag
   103621 ctacgccagc gcggattgtt cccggttgta gcggtagagc tggagttcta tttactggat
   103681 cgtcagcgcg acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc
   103741 aatacacaaa gccaggttta ctccgttgat aacctcaacc acttcgccga tgtgctcaat
   103801 gatattgatg aactggcgca gttacagtta attcccgccg acggtgcggt cgccgaagcc
   103861 tcgccgggtc agtttgaaat caacctttac cataccgata acgtgctgga agcctgcgat
   103921 gatgcgctgg cgctaaaacg cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc
   103981 acttttatgg cgaagccgta tgaagagcac gcgggcagcg gaatgcatat ccatatcagt
   104041 atgcaaaata accgtggcga gaacgtgctt tctgacgcgg aaggcgaaga ttcgccgctg
   104101 ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc gttgctggca
   104161 ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg
   104221 tggggccata acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg tcataatcac
   104281 cgcgtggaat atcgcgtggc gggtgccgat gccaacccat atctggtgat ggcagcgatt
   104341 tttgccggta ttttgcatgg ccttgataac gagctgccgt tgcaggaaga agtcgaaggc
   104401 aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctgggtgag
   104461 tttatcgaga atgatcacct gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac
   104521 gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag
   104581 tggatgttga aaaacgcgta attccccatg cggggttgtt tcatcggcct gagcggagcc
   104641 aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcaggc
   104701 ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat
   104761 taaatccccg catggcgagg gaggctgcgt atgcgcccct cctgctactg actggactcc
   104821 gccccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac cgcaaacctt
   104881 attacgcagg ggaggcagca attatggcta ttaattcacc actgaatatt gctgcgcaac
   104941 ccggcaaaac ccgtctgcga aaatcactga aattgtggca ggtggtaatg atgggtctgg
   105001 cctatctcac gccgatgacc gtatttgata cttttggtat tgtctccggc attagcgacg
   105061 gtcacgttcc agcgtcctat ttgctggcgc tggcgggcgt gctgtttacc gctatcagct
   105121 acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt
   105181 cgattaatcc gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt
   105241 tgccaatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc
   105301 cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg
   105361 tcaacctggt cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg
   105421 tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt
   105481 cacttcagcc gtttatcagc gagaacgcgc acctgatccc gattattacc ggtgcgacga
   105541 ttgtctgttt ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa gagacgccgg
   105601 acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtatacggc ggcgttatct
   105661 ttatcgcggc gtcgttcttt atgcagctgt tctttcccga tatcagccgc tttaaagacc
   105721 cggacgccgc actgcctgaa attgcgctgt acgtcggcgg taagctgttc cagtcgattt
   105781 tcctttgcac cacgtttgtg aacacgttag cgtctggcct ggcctcacat gccagcgtgt
   105841 cacgtctgct gtatgtgatg gggcgcgaca atgtgtttcc ggagcgcgtg tttggctatg
   105901 tgcatccgaa atggcggact ccggcactga acgtcattat ggtcgggatt gtcgcgatgt
   105961 cggcgctgtt cttcgattta gttaccgcca cagcgttaat taacttcggc gcgttggtag
   106021 cgtttacctt cgttaacctg tcggtgttca accacttctg gcggcgcaaa ggaatgaata
   106081 aaagctggaa ggaacacttc cactatttat taatgccgct ggttggcgcg ctgacggtgg
   106141 gcgtgctgtg gattaacctc gaagccacct cgctgacgct gggtctggta tgggcttcgt
   106201 taggcggcgc atatttgtgg tatctgatcc gccgctatcg caaagtgccg ctgtacgaag
   106261 gtgacagaac gccagtgagc gaaacgtaat agtcggatgc gcgccgcatt atcctgcctg
   106321 agggtaatgc gccgcgccat taactataaa aagatgtatg acccggatca cgagtgctat
   106381 catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg
   106441 ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac
   106501 gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat
   106561 gaagagatca gcgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta
   106621 cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc
   106681 agacgatcgg tcagtatcgt tgtccggata aagcgttcac gccgcatccg gctaacaatg
   106741 acacgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta
   106801 agttgttctg tgagttatag ttatagagaa atcacgggta cgaatgttga acagatggcg
   106861 acgccacctg ttcaacgacc aaccacacgg ccctcattac gacccctgaa cacaaacggt
   106921 aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt
   106981 atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc
   107041 gccgcgcctg aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc
   107101 agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc
   107161 cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg
   107221 ccggaacttg ccgaaagctg ggaagtactc gacaacggcg cgacctatcg cttccacctg
   107281 cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc
   107341 gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc
   107401 aacggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc
   107461 cgcaaactgg ataatcatac cgttgagttc cgactcgccc agccggatgc ttcttttttg
   107521 tggcacctcg caacccatta tgcttcggtc atgtcggcag aatatgcccg gaagttagag
   107581 aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcagttg
   107641 tcggaatacc gcgccgggca atttattcgc ctacaacgtc atgatgactt ctggcgcggt
   107701 aaaccgttaa tgccgcaggt agtggtggat ttaggctccg gcggcaccgg acgtctgtcg
   107761 aaactcctga ccggggaatg cgacgttctg gcctggcctg ctgccagcca gctatccatt
   107821 ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg
   107881 gcatttaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc actggcactg
   107941 gcgattaata accagcgcct gatgcaatcc atctattatg gtacggctga aacggcggcc
   108001 tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacaat
   108061 ccggcgaaat cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg
   108121 tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaactgc cgaactgatt
   108181 caggcggata tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt
   108241 caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac
   108301 agtaacgacc cggacagttt cttccgtccg ttactgagct gcgcggcaat tcattcacag
   108361 accaacctcg cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc
   108421 tcgcagcagc tggcagcgcg tattgaagcc tatgacgaag cgcagagtat tctggcgcag
   108481 gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc
   108541 aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa
   108601 caggatgagg tgaaaaaacc atgattatct tcaccttacg ccgcattttg ttattgattg
   108661 tcaccttgtt cctgctgacc ttcgttggct tcagtttgag ctatttcact ccacacgcgc
   108721 cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaacggc ctgatccact
   108781 gggattttgg tgtttccagc attaacggtc aaccgattgc cgaacagtta aaagaggtct
   108841 tccctgcgac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc
   108901 ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca
   108961 tcgccttgct gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt
   109021 gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctg tacgaagtga
   109081 aaccgattac cgggtttgcg ttgattgatg cctggctttc ggactcacca tggcgggatg
   109141 aaatgatcat gagcgcaatc cgccatatga tattgcccgt gatcaccctg tcggttgcgc
   109201 caacaactga agtgatccga ctgatgcgta tcagcactat cgaagtgtat gaccagaact
   109261 atgttaaagc ggcggctacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc
   109321 tgcataacgc gttaccgccg gttattcctc gcctgggctt acagttttcc accatgctga
   109381 cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta
   109441 acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac
   109501 tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac
   109561 ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg
   109621 cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc
   109681 gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag
   109741 caatttctcg gttaccaatt attgccgccg tcatggtcgc gctatggcga agtttctttc
   109801 ttcctgggga ctgacgacct cggacgcgat gttttaagcc gtttactgag cggagctgcg
   109861 ccaaccgtgg gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg
   109921 ggcaccttcg ccggggcgac gcacggccta cgctcagcgg tgcttaacca tattctggat
   109981 accttactgg cgatcccttc gctgctgctg gcaattatcg ttgtagcgtt tgccggaccg
   110041 agtttgtccc acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg
   110101 atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat
   110161 ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc caaacatcac cgccgggctg
   110221 gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt
   110281 ctcgatctcg gcgcacaact cccctcgcct gaatggggag cgatgctcgg tgatgcgctg
   110341 gaactgattt atgtcgcccc gtggactgtc atgctgccag gtgcggcaat tatgattagc
   110401 gtgttgttgg ttaacctcct tggcgatggc gttcgtcgtg cgattattgc gggggtggaa
   110461 taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg
   110521 ttaaagccgt cgaccgggta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg
   110581 gtgaatccgg ttccggcaag agtttgattg cgaaagcgat ttgtggggtg aataaagata
   110641 actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg
   110701 cacgcgaacg gcgcaaactg gttggccata acgtgtcgat gattttccag gaaccgcagt
   110761 cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga
   110821 cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc
   110881 tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga
   110941 ccgaaggtga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc
   111001 tgattgctga cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa atctttcgcc
   111061 tgctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac
   111121 aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acagtggaaa
   111181 ccgcgccgag taaggagttg gtgacgatgc cacatcatcc ttatacccag gcgctgatcc
   111241 gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg
   111301 gcgctatccc actgctggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt
   111361 atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg
   111421 cctgtcattt cccgctgaac atggagaaag agtgagatga tcgaaacgct gcttgaagtg
   111481 cgtaatttaa gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa
   111541 gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat tattggcgag
   111601 aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga gcctaccagc
   111661 ggcgagttat tgattgacga tcatccactg cattttggcg attattcctt ccgcagtcag
   111721 cgtattcgca tgatttttca ggatccttcg acctcgttga atccccgtca acgtatctcg
   111781 caaattctgg attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa
   111841 cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg
   111901 catatgttgg cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc
   111961 ccaaaagtca ttattgccga tgaagccctc gcttcactgg atatgtcgat gcgttcgcag
   112021 ttgattaatc tgatgctgga gttacaggaa aaacagggca tttcgtatat ttatgttacc
   112081 cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag
   112141 gttgtcgagc gaggcagcac cgcggatgtg ctggcatcac cgctgcatga actcaccaaa
   112201 cggctgattg ccgggcactt tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc
   112261 taattttctg aatcctcctt ccagattatt cacctcattc tgacaagaga ttaaatcttg
   112321 tcagaatgag gtgatggata aaataaaatc aaatgcgcgt gatttaagaa gcaatctcac
   112381 tttgcaggaa cgaaagctct ggcgatatct tcgcagccga cgttttggtg atttcaaatt
   112441 tcgccgtcaa catccggtgg ggagctacat tctcgatttt gcttgctgct cggcgcgtgt
   112501 agtcgttgag ctggatggtg ggcagcatga tttggcagtt gcctatgatt ccaggcgcac
   112561 tagctggctt gagtcgcagg gctggaccgt gctgcgtttc tggaataacg agattgattg
   112621 taatgaggag acggtgctgg agaatattct gcaggaactg aaccgccggt caccctctcc
   112681 ctgaaagagc gagggggccg accgagccga atagctgttg tggtgaaaat atggtgacag
   112741 ttctggagaa tattcggcaa ggtctgaacc gtcccagcca tcgccctgaa agggttaggg
   112801 ggctgtataa gcctgtttgt tgctggggtg acaatatttg cacaatacgg tcccctcgcc
   112861 cctctgggga gaggattagg gtgtggggaa aagcgcccac cctgcccgca ccctgctccg
   112921 gtcggacctg gcagctatag ccactcacag ccaggttgat tataataacc gtttatctgt
   112981 tcgtactgtt tactaaaacg acgaatcgcc tgattttcag gcacaacaag catcaacaat
   113041 aaggattaaa gctatgggtt ttctttccgg taagcgcatt ctggtaaccg gtgttgccag
   113101 caaactatcc atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag ctgaactggc
   113161 attcacctac cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg ctcaattggg
   113221 ttctgacatc gttctgcagt gcgatgttgc agaagatgcc agcatcgaca ccatgttcgc
   113281 tgaactgggg aaagtttggc cgaaatttga cggtttcgta cactctattg gttttgcacc
   113341 tggcgatcag ctggatggtg actatgttaa cgccgttacc cgtgaaggct tcaaaattgc
   113401 ccacgacatc agctcctaca gcttcgttgc aatggcaaaa gcttgccgct ccatgctgaa
   113461 tccgggttct gccctgctga ccctttccta ccttggcgct gagcgcgcta tcccgaacta
   113521 caacgttatg ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata tggcgaacgc
   113581 gatgggtccg gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc gtactctggc
   113641 ggcctccggt atcaaagact tccgcaaaat gctggctcat tgcgaagccg ttaccccgat
   113701 tcgccgtacc gttactattg aagatgtggg taactccgcg gcattcctgt gctccgatct
   113761 ctctgccggt atctccggtg aagtggtcca cgttgacggc ggtttcagca tcgctgcaat
   113821 gaacgaactc gaattgaaat aatcattctg ttggtaaata ttgggcggcg ttctgccgcc
   113881 cgttatctct gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc
   113941 cgccttgcgt caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca
   114001 tatcaccggc aaaagccact ggtatcatga aacgcaatcc agtactgcgg agtatgacgt
   114061 tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct
   114121 tgatgaagaa acgctggctc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt
   114181 ggaattgttc cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg
   114241 cttgtctacg gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta
   114301 ttcggcgcga cttacgccgc tccccgggcc tgattcctcc agggaaagca atcataggtt
   114361 ggcacaaatc acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg
   114421 atcgcgccag catctgaatg acgtcggtct tactgccggg gactgtgtga tcattaacca
   114481 aatcattggt tttattggct ttcaggcgcg gaccattgcg acatttcagg cttatctcgg
   114541 gcacccggta cgctggttac ccggtctgga aatacaaaac tacgccgacg cgtcactgtt
   114601 tgctgatgaa tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagta
   114661 cacaaaaagt tcaacagcag aactttgcca actggccgaa acactctctc tccaccctat
   114721 ttcactttcc cttctcgaaa ggttgttaaa cagtacacgg gttaatacac agccggataa
   114781 taagcttgcg gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg
   114841 tatggattca tcaaatgaat ataaaaaaat cagccccctt ctgcgcaagg gcgaaaatga
   114901 aattaaccaa tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg
   114961 gctgacccga gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga
   115021 aaaatcatca acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa
   115081 tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt
   115141 aaaactgtca gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc
   115201 tgctagcgca gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa
   115261 aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc
   115321 cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg
   115381 aaaaagaacg tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg
   115441 tgggtaaagt tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa
   115501 aagacgccat tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact
   115561 gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac
   115621 tgacacaata catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc
   115681 gccataatct ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc
   115741 tggttcgtga agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag
   115801 atatggatga cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg
   115861 cgattgccga tccaaccgcg tggattgcag aaggcagcaa gctggacaaa gccgcgaaaa
   115921 ttcgcgcatt caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt
   115981 ctgacgatct ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga
   116041 cgctctccgc tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat
   116101 ccaaagcgaa gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc
   116161 agcctgaaag tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc
   116221 gcggtgagtg gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta
   116281 ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc
   116341 gtatcgtcga agaagcgatg attgccgcta acatttgtgc ggcccgcgta ctgcgcgata
   116401 agctcggttt tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc
   116461 tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg
   116521 acggtttctg caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc
   116581 gcattcgtcg cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc
   116641 tcggtctgga agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca
   116701 accaccgtct gctgaaagcg gttatcaaag gcgaaactgc gacgcgccca caggatgaaa
   116761 tcactgtcca aatggccgag cgtcgccgtc tcaaccggat ggcagaacgt gatgttggtg
   116821 actggttata cgcacgcttc ctgaaagaca aagccgggac cgacacccgt ttcgcagcgg
   116881 aaattgtcga tatcagccgt ggcggcatgc gtgttcgttt ggttgataac ggcgctatcg
   116941 cctttattcc ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa
   117001 acggcactgt acaaattaaa ggtgaaacgg tttacaaagt gactgacgtt attgacgtca
   117061 ctattgccga agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc
   117121 ctttcacggc ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat
   117181 ccaggcagat tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta
   117241 caaataaata tataaaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt
   117301 tgttcctttt atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc
   117361 gttagggagt ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg
   117421 acggaaagca gcgatgtttt gcgcttttct accaccgaaa ccacagaacc tgatcgtact
   117481 ttgcagttat ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc
   117541 ctgatgatga gtctgaccgt cgatgagagc gatctttctg tgcatctggt aggacgaaaa
   117601 atcaataaac gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcggttgct
   117661 cgtgatttat cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca
   117721 atagtcattc tcgacagccg ggggaatatt caacgcttca atcggttatg tgaagattac
   117781 acagggttga aagaacacga cgtcattggg caaagcgtgt ttaaactgtt tatgagccgt
   117841 cgtgaagctg cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat
   117901 gaagtcgaac tgtggatacc aacatgtaaa ggccagcggc tgtttctgtt tcgcaataaa
   117961 tttgtccaca gcggcagtgg caaaaacgag atttttttaa tctgttccgg caccgacatt
   118021 accgaagagc gccgcgctca ggagcgactg cgtattctgg caaataccga cagtatcacc
   118081 ggactgccga atcgtaacgc aatgcaggat ttaatcgatc acgctattaa tcatgcagat
   118141 aacaataaag ttggggttgt gtatcttgat ttggataatt tcaaaaaggt caacgacgcc
   118201 tatgggcatt tgtttggtga ccagttatta cgcgacgtgt cattggctat tttaagctgt
   118261 ctcgaacatg accaggtgtt ggcgcgtcca ggtggcgatg agtttctggt actggcatcc
   118321 aacacctcac aaagcgcgct ggaagcaatg gcatcacgaa ttttgacccg cttacggctc
   118381 ccctttcgca ttggtttaat tgaagtttat accagctgtt cagtaggtat tgcactctct
   118441 cccgaacatg gttcagacag cacggctatt attcgtcacg ccgacacagc aatgtacaca
   118501 gcgaaggaag gcggacgagg acaattttgc gtttttaccc cagaaatgaa tcaacgggta
   118561 tttgaatatc tctggctgga taccaacttg cgtaaagcac tggaaaacga tcagttggtt
   118621 attcactatc aaccgaaaat cacctggcgt ggcgaagtgc gcagtctgga agcactagta
   118681 cgttggcagt cacctgaacg tgggttgatt ccaccgttgg acttcatttc ctacgccgaa
   118741 gagtcagggc taattgtgcc tttaggccgt tgggtgattc tcgatgtcgt acgccaggtg
   118801 gcgaagtggc gggataaagg tattaacctg cgagtggcgg taaatatttc tgcacgtcag
   118861 ctcgccgatc aaaccatttt caccgccctg aaacaggttc tccaggaact caattttgaa
   118921 tactgcccta tagatgttga actgacagag agttgtctga ttgagaatga tgaactggca
   118981 ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag tgcatctgga tgattttggt
   119041 accggctact cttcactttc gcaactggcg cgctttccga tcgatgccat caaacttgac
   119101 caggtttttg ttcgagatat tcacaaacaa cctgtctcgc agtcactggt ccgggcgatc
   119161 gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg aaggtgtaga gagtgcaaag
   119221 gaagatgctt ttttaaccaa gaacgggatc aatgagcggc aaggattttt gtttgccaaa
   119281 ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct atctgaagcg cgcataagta
   119341 gatgaatata tatacttagc gctatatccc gtcattatat atcgtattcc tggatgataa
   119401 ttagccgttg ctgttaaaca cttctctttt agcggcaatt tttgcattat tcattttcca
   119461 cttttgctat aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta
   119521 ttgatattgt gttagatatc cttgtggcag gcgattacca ctctgcgatc cataatctcg
   119581 agatacttaa agcagaactc ttgcgccagg ttgctgaatc tacgccagac atcccgaagg
   119641 caccgtggga gatttaacaa tcgtgccttc aggttttcct atttgaggat gcccggcgcg
   119701 tcagtatttt tttatttagt attataacgt tataagaatt acagcgatga attcccgaca
   119761 acaaactatt ctacagatgg tcattgacca gggtcaggtt agcgtaaccg atctggcaaa
   119821 agccactgga gtttctgaag ttaccattcg ccaggatctc aacaccctcg aaaaactgag
   119881 ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat agtgatgacg tcgaaacccg
   119941 tatgatgagc aactatacgc tgaagcgtga actcgccgag tttgccgcgt cactggttca
   120001 accgggcgaa accatcttta tcgaaaatgg tagcagcaat gccctgctcg cccggactct
   120061 gggcgaacag aagaaaaatg tcactatcat cacagtcagc agctacattg cgcatttgct
   120121 gaaagacgcg ccttgtgaag tcattttgct cggtggcgtg taccagaaaa aaagcgaaag
   120181 tatggttggc cctttgacac gccagtgcat ccagcaggta catttcagca aagcatttat
   120241 tggtattgat ggctggcaac ctgaaactgg attcaccggt cgcgatatga tgcgtaccga
   120301 tgtggtcaat gccgtgctgg aaaaagagtg cgaagcgata gtcctgactg acagctcgaa
   120361 atttggtgct gtacattcat actccatcgg tcccgttgag cgattcaatc gcgtgattac
   120421 cgattcgaaa atacgcgcca gcgatctgat gcatcttgag cacagtaaac tcaccgttca
   120481 cgtcgttgac atttaaccat ttcccttctc tacggatgat ttgcagtttg gcaaatcatc
   120541 cgctctaaga tgattcctgg ttgataatta agactattta cctgttatta acactctcaa
   120601 gatataaaat tattatcagc gatataacag gaagtcatta tcacctgcgt gatataaccc
   120661 tgcgcgcgag cagatttcac ggaataattt caccaggctt attcttagct attatagtta
   120721 tagagagctt acttccgtga atcataaatt caggagagag tattatgttt gtaacgagca
   120781 aaaaaatgac cgcggctgtt ctggcaatta ccctggcaat gtctctgagt gcctgttcta
   120841 actggtctaa acgggaccgc aacaccgcaa tcggcgcggg tgcaggggca ttaggcggtg
   120901 cagtactgac cgatggcagt acgttgggta cattaggtgg tgcagctgtc ggtggtgtta
   120961 ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga ttatcgactc gcaattataa
   121021 tacgcttgat tctgttcagt acgaaataac tgaataatac acacagtaaa atattcgaaa
   121081 gccacggata tatccgtggc tttttatgtt aaccacctgc gagttttacc ttcatccctt
   121141 tcgcttcaag tagtgatttt aataaatcac gcttatcgcc ctggatttca ataactccat
   121201 ctttaactgc tccgccgcag ccgcattttt tcttcagttc cgctgcaagt tttgtcagtt
   121261 cggcatcatc gagatcgaca ccggtaatca ggcaaacgcc cttaccttta cgtccactgg
   121321 tctgacgctg aatacgcacc acaccgtcgc ctttagggcg tacaggggcc gctttgggct
   121381 catcaatacg tccggtttcc gttgagtaga ccagacggct gttggaatca ctcatcatgc
   121441 actccgctgt aaagaggcgt tgatcgcttt cagcgtctgc gctggatcta ccgattgcgt
   121501 taccgggcga ccaatcacca tataatcaac accagccgac aacgcctgtt ctggcgtcat
   121561 aatgcggcgc tggtcaccag cttcactccc ctgcggacga atgcccggcg taaccagttt
   121621 gaactcctga ccgaatacct gtttaaagcg cacagcttcc tgagcagaac acaccacacc
   121681 atcaaggcca catttttgcg tcagtgccgc cagacgttct gcataatctg caggtgacag
   121741 tgtcatgcca agatcgacca ggtcgctggc ttccatgctg gtcaacactg tcacagcaat
   121801 caaaagcggt gcatctttgc caaacggaac cagtgcctca cgcgctgcgg tcatcatacg
   121861 cgccccacca gaggcatgaa cattcaccat ccacacgcct aagtcagctg cagcagcgac
   121921 agcgtgcgca gcagtgttag gaatatcgtg gaatttcagg tcaagaaaga tatcaaaacc
   121981 acgctgttga agttcgcgca caaactgtgg cccaaacaat gtaaacatct ctttgccgac
   122041 cttcagacga caatcgcgtg gttcgatctt gtcgacaaag gacagcgcgt catcacgatt
   122101 atgataatca agggcaacaa ccacaggaga attcgtaaca gcgcgggaag aagatgaagc
   122161 agttaacgtc atgaccagac cttcttgatg atgggcaccg gaagtggcgc gaaacaggta
   122221 aacggcgagc attctacccg tggaaataaa aaattaacag ttgcgatttt tcccctggca
   122281 ggctttttct gcgccgtcgt tgccggagca caatggaaca tcaataacta gtatgttgta
   122341 actaaagtag gattttttta aaaattacag gccatcaaga ccgcgaatcg gtttaatggt
   122401 tgaccaggcc cgacaagacg gacaatgcca gtagagggtg tatgcggtaa aaccacattt
   122461 ctggcagcga taacgaggct tactacgtac cttttcgcca accatgtcac gcagcaccat
   122521 caggctctct ttggcacgcc cttcttccgc ttcatttaag tggtaatcca ttaacttatg
   122581 gaacacacgc atggtcggat gacgctgaag ctggcgcgta atatagacct gtgcggcctc
   122641 actaccgtcg cgcgcttcga tgatatcagc aagcatcaat tcagcatcgg caccggtgtt
   122701 ctcttccacc gcgcgttgca ggaattctgc ccattcggca gttttaccca actgctgata
   122761 gcaggtttgc aacatttcca gcgtttcgct gaccagttct ctgtcctggg atatcacgcg
   122821 ttgcaaactt tcgacggctt tggcgtattc tcctttcgcc ataaacacgc gtcccatcat
   122881 gatggatacg cgggcgctgt ttttatctgc cgccgcccct ttttttagca aggtcatggc
   122941 acgatcgaga tcgtcgctgg ccatatgctg cagggctaac tcacagtaga aatgggcaat
   123001 ttcgacgcgc tgtttatctt tacccagctt caccaggcgt tcggcaacat caattgcttt
   123061 ctgccactca ctggtagcct ggtagatttg tagcaactgt tgcagagcgc caatgcggaa
   123121 gtcagtttca tcggtcagct gattgaacat gtcttccgcg cggtcgtata acccggcggc
   123181 catgtaatca cgccctagtt gttgaatcgc caacagacgc tgttcatagg tcagcgaggc
   123241 gctttccatt agggtctgat ggatgcgaat agcgcgatca acttcgccac gcgaacggaa
   123301 caggtttccg agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa gcatatcgag
   123361 aaacaggtct accgctttat cctgttgatt actaagcagg aagttaaccc ccgctacgta
   123421 atcacgcgac aagcggttgg cttcatcttg cttgttttgt tgcgcacttc tgcggcccat
   123481 ataccagcca taggcagcgg ctacaggcaa aagcagaaac aacaactcca gcatagaaag
   123541 ttattccttc gccgctgacg agtgcggcac tacagccacg tcagtcgcgg gtgaaagctg
   123601 gttttccagt cgctttattt tacgttcagc gcgcgccagg gaaacacgaa ctcgcagcca
   123661 gaacaggcca caaatcaacc aaccgatagc aaaccccgca gcaaacaata ccgccagcaa
   123721 tgtggaaata cggtactccc cttgcgctaa cagataatta aacgtcacct gttgatcgtt
   123781 ctgcgcaccc aacgtgaccg aaatcacgaa gatcgctaac accagtaaga aaatgagtaa
   123841 atatttcaca ttacttcccg ttatgtggtt agagcgaata aattatggtc aacttaccgc
   123901 aatagccctt taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt
   123961 gatgatttaa gggctaaatt cggaaaatca accagcgtta actttcttgt tctcgttgcg
   124021 ctatttcgcg attttcttcc gcaggtggtg ttaatggccc acaaattcgt tgcgcaagcc
   124081 acgttgccac cgccaccagc gcccacgaaa tcaacgtagc tactaccaga tcgcgtggcc
   124141 aatgcatccc gagcagcagg cggcttccca tgactcccgt tgcccagacc agcaagatag
   124201 caatggttaa cgttcgccga cgcggccaca gcaaaccaac ggccagcagt gcccaactgg
   124261 cagcaaacat cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc cagtgtgaac
   124321 gcaaatattg tgggatattt ttctcttcag ccaactgttc tttcactaga tttccgcgtt
   124381 ctgctcgctt taaagtgtag aactcatcaa ccggaatatg atgtgttttt tccagccaga
   124441 taacaaaagg tcgtggttcc tggactttgt ctttgatcca ggatttaacg ccttgtccca
   124501 caaggattgc ggccgccaga atggcaaata atacaaaggc agccttaatg cgaaaacgca
   124561 gacaccagag aaaccagccg aataaaatca aatgtgtaat gacgccccag ggctgggtga
   124621 cagtttcagt aacccaaaaa gccgctttta gtagccaact ttgttctcca ggttgccaac
   124681 gccagccaga aatccatacg gctactggca tgacaagcaa tagtgcagct cccactgcgg
   124741 tacgtctggc aatcgaacgc atggcctctc ctttttgata agtcccacaa tcataactga
   124801 aaacgccagt tccaggaaaa attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc
   124861 gtgattaggt gaaccccttc tcgttatggc aaaataagcc aatacagaac cagcattatc
   124921 tggagaattt catgcagctt aaacgtgtgg cagaagccaa actgccaacc ccatggggcg
   124981 atttcctgat ggtgggattt gaagaactgg caaccggaca cgatcatgtc gcgctagtct
   125041 atggcgatat ttccgggcat accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg
   125101 gtgacgccct gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc
   125161 aaattgccga ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg
   125221 gtctgctgaa taaaatccgc gcttacgcac tgcaggatca aggttacgat accgtagagg
   125281 ctaaccacca gttaggcttc gccgctgatg agcgcgactt cactctttgc gctgatatgt
   125341 tcaaactcct tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa
   125401 ttctgaccga agcagggatt aatattgttg aacgcgtacc attgattgta ggtcgtaacc
   125461 ccaataacga acattatctc gataccaaag ccgagaaaat gggccatttg ctgaacaaat
   125521 aaccctcttg cattgtgtaa ttagtttgct tgccggaagc aaaataaccg gcaagcaaat
   125581 acttgttact tcaacatatt acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtag
   125641 gtcaactccg tcgcggtgtc gatacgacaa cggcagggta cgacctcctg gctaccatcc
   125701 gcgcgcgtaa gcgtcaccgg aaccgtcgca ccgggttgta gattttgcag atcgccaata
   125761 tcaatcttct cttccccggt tagtcctaac gttttacgcg ttacgccttg cggaaattcc
   125821 agcggcagga tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa cgattcggca
   125881 atcaccacac gaattccaag cagacgagga ccttttgccg cccagtcacg gcttgagcct
   125941 gatccatact ctttcccggc aatcaccgcc agcggcgttt gttcctgctt atagcgcatc
   126001 gcagcatcat aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat ccccccttca
   126061 acgccaggca ccatttcatt acggatgcga atattggcga aggtgccgcg catcatcact
   126121 tcatggttac cacgccgcga accgtaggag ttaaagtctt ttcgctcaac accccgacct
   126181 tgtagatatc gacccgctgg gctgtcgggc ttaatactgc ccgccggaga gatatggtca
   126241 gtggtgactg aatcccccag cattgcgagg atccgcgcac cgtgaatatc ttccactggt
   126301 gctggtgttg cctgcatttc atcaaagaaa ggcgataagc gaatataggt tgagtcctcc
   126361 tgccaaccgt aggtatcgga tcgtgtgacg ttaatttcct tccactctgc tgtgccttca
   126421 aaaacttctg cgtactcttt gcggaacatt tctgtggaga cttgttctac cgcacgggca
   126481 atttcttgtg ccgatggcca gatatctttc agatataccg gatcgccttt acgatcatgg
   126541 ccgataggct cagaagccag gttgatattc atatttcccg ccagcgcata ggcaaccacc
   126601 agcggcggcg aggccagcca gttggtttta accagcggat ggatacggcc ttcaaagtta
   126661 cggttgccgg acagcactgc accgacggtt aaatcgcctt ttttgattgc cgtttcgata
   126721 ggatcgggca gcggcccaga gttaccaata caggtggtac aaccgtatcc cacaaggtta
   126781 aaccccagtt cgtcgagata cggtgtcaat ttcgcttttg ccagataatc agaaacaact
   126841 ttcgagcctg gtgccagcga cgctttgacc cacggttgcc gcttgaggcc aagagttacg
   126901 gcttttttcg ccagcaagcc tgcagccatc agcacacttg ggttagaggt gttggtgcac
   126961 gaggttatcg cagcaatgac cacagcgcca tcaggtaact gatactgatg tccgttcata
   127021 acataatcga ccggctggcg atctttatgc gtggcattca cttccagttc gttactggcg
   127081 gcaaatgctt ttggtacatc gggcagtgca acgcgatcct gtgggcgttt tggccctgcc
   127141 aggctcgctt caacgtcatt catatccagt tctaacgtac tggtaaaaat cggttcatcg
   127201 cccgggttac gccacatgcc ctgcgctttg gcatattttt cgaccaactc aacctgatct
   127261 tcgctgcgcc cgcttaaacg catgtaatcg agggttacag catcgattgg gaagaagcca
   127321 caggtggcac catattctgg cgacatattg gcaatggtgg cgcgat
//
LOCUS       DAFATA010000004        91132 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFATA010000004 DAFATA010000000
VERSION     DAFATA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 91132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.004"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..961
                     /gene="gatZ"
                     /locus_tag="LGJ20_000461"
     CDS             <1..961
                     /gene="gatZ"
                     /locus_tag="LGJ20_000461"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBK2384586.1"
                     /translation="AMEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERA
                     AVLCFAAESVATDCQREQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKA
                     FIARGLTEALTRVIAIVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDY
                     QTRTAYWELVRDHFAILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVM
                     LDEPQYWKKYYRTGFNDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLG
                     MISQYLPKQFERIQSGELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            971..1423
                     /gene="gatA"
                     /locus_tag="LGJ20_000462"
     CDS             971..1423
                     /gene="gatA"
                     /locus_tag="LGJ20_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBK2384587.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            1454..1738
                     /gene="gatB"
                     /locus_tag="LGJ20_000463"
     CDS             1454..1738
                     /gene="gatB"
                     /locus_tag="LGJ20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBK2384588.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            1742..3097
                     /locus_tag="LGJ20_000464"
     CDS             1742..3097
                     /locus_tag="LGJ20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK2384589.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            3146..4186
                     /gene="gatD"
                     /locus_tag="LGJ20_000465"
     CDS             3146..4186
                     /gene="gatD"
                     /locus_tag="LGJ20_000465"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK2384590.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            4292..5065
                     /locus_tag="LGJ20_000466"
     CDS             4292..5065
                     /locus_tag="LGJ20_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2384591.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(5147..6046)
                     /gene="yegS"
                     /locus_tag="LGJ20_000467"
     CDS             complement(5147..6046)
                     /gene="yegS"
                     /locus_tag="LGJ20_000467"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBK2384592.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            6461..6778
                     /locus_tag="LGJ20_000468"
     CDS             6461..6778
                     /locus_tag="LGJ20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384593.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(7043..8056)
                     /locus_tag="LGJ20_000469"
     CDS             complement(7043..8056)
                     /locus_tag="LGJ20_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2384594.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPTGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(8172..8471)
                     /locus_tag="LGJ20_000470"
     CDS             complement(8172..8471)
                     /locus_tag="LGJ20_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2384595.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            8593..8868
                     /locus_tag="LGJ20_000471"
     CDS             8593..8868
                     /locus_tag="LGJ20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HBK2384596.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            8879..9049
                     /locus_tag="LGJ20_000472"
     CDS             8879..9049
                     /locus_tag="LGJ20_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384597.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            9046..9546
                     /locus_tag="LGJ20_000473"
     CDS             9046..9546
                     /locus_tag="LGJ20_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HBK2384598.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            9610..9834
                     /locus_tag="LGJ20_000474"
     CDS             9610..9834
                     /locus_tag="LGJ20_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HBK2384599.1"
                     /translation="MHTVSENQCGIYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGKVL"
     gene            9834..10136
                     /locus_tag="LGJ20_000475"
     CDS             9834..10136
                     /locus_tag="LGJ20_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HBK2384600.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            10136..10360
                     /locus_tag="LGJ20_000476"
     CDS             10136..10360
                     /locus_tag="LGJ20_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBK2384601.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGVSSLVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            10357..10632
                     /locus_tag="LGJ20_000477"
     CDS             10357..10632
                     /locus_tag="LGJ20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HBK2384602.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLSVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEVFGSYGS"
     gene            10622..12901
                     /locus_tag="LGJ20_000478"
     CDS             10622..12901
                     /locus_tag="LGJ20_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBK2384603.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKTFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERLQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWSGFWHQFE"
     gene            13028..13219
                     /locus_tag="LGJ20_000479"
     CDS             13028..13219
                     /locus_tag="LGJ20_000479"
                     /inference="COORDINATES: protein motif:HMM:NF021447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBK2384604.1"
                     /translation="MLKPADVVCLKSGGFKMTVSDSTETHAKCIWHDKEGVLHEESFS
                     IDLLEIAESKSGMLCRPRK"
     gene            13247..13927
                     /locus_tag="LGJ20_000480"
     CDS             13247..13927
                     /locus_tag="LGJ20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384605.1"
                     /translation="MKQYSELENNVKRFIVEHEKGISIDDIHHKFRMKDGQNRKMADY
                     LIDNKKIILEMKSLFSDRVKNVNDKLNELVKTDSWLAKNWHGAIHLEELIKRHPDSKR
                     FRNDIMNFAYENIKTKIVKEANKQINATKDVLDLNDSIGGLILLNDNVFSHESNYLSD
                     EILYLLGTKRYSSVEFVVYIAKLIDKQVDVCVLLDSQSKNKNYIEWYMNNVFLLNWAS
                     FNKYAIKM"
     gene            14022..14174
                     /locus_tag="LGJ20_000481"
     CDS             14022..14174
                     /locus_tag="LGJ20_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meiotically up-regulated 80 protein"
                     /protein_id="HBK2384606.1"
                     /translation="MAIVHRLQQIMVNENLTPDELVGCAEIVWDNYGRFNYIGQSRVA
                     PPPRRR"
     gene            complement(14247..15188)
                     /locus_tag="LGJ20_000482"
     CDS             complement(14247..15188)
                     /locus_tag="LGJ20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384607.1"
                     /translation="MQGEVDEQQPNEIISEFGYYPVEVNIETEQFSLRTLPGLIENVE
                     RINNDKNVVNGWIYPGNRKVYNLNGDTCTMPYSYRVFGMPKTHTLKLKNTSSLETLNF
                     VVWCLSFFKGIRLTTTDAGFLDATTIKPTKLTDFILVGCSEKEVIELALNYIKDKQKD
                     DRSIKRIAAVIHSLFLSHNPLYLSFEKFQYLYMALDCCFAIAWDERNKVIKEKKPSHQ
                     NRVYWMCENYGVKIPSWATDECNVSVIRNDNFHEAIFNGQPLGFSSINDCQYGSDILL
                     QMQALVCRLLAAILSVNDRKYIASTISSIEYHSLKLT"
     gene            complement(15631..16665)
                     /locus_tag="LGJ20_000483"
     CDS             complement(15631..16665)
                     /locus_tag="LGJ20_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2384608.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRICEINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(16665..18437)
                     /locus_tag="LGJ20_000484"
     CDS             complement(16665..18437)
                     /locus_tag="LGJ20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HBK2384609.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGSDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            18611..19465
                     /locus_tag="LGJ20_000485"
     CDS             18611..19465
                     /locus_tag="LGJ20_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBK2384610.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALLTNC"
     gene            19524..20597
                     /locus_tag="LGJ20_000486"
     CDS             19524..20597
                     /locus_tag="LGJ20_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBK2384611.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTQARATAEPGA"
     gene            20601..21344
                     /locus_tag="LGJ20_000487"
     CDS             20601..21344
                     /locus_tag="LGJ20_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HBK2384612.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            21443..21952
                     /locus_tag="LGJ20_000488"
     CDS             21443..21952
                     /locus_tag="LGJ20_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBK2384613.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            21952..22155
                     /locus_tag="LGJ20_000489"
     CDS             21952..22155
                     /locus_tag="LGJ20_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HBK2384614.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            22159..22440
                     /locus_tag="LGJ20_000490"
     CDS             22159..22440
                     /locus_tag="LGJ20_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK2384615.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLSAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKQ"
     gene            22440..22937
                     /locus_tag="LGJ20_000491"
     CDS             22440..22937
                     /locus_tag="LGJ20_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HBK2384616.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            22952..23377
                     /locus_tag="LGJ20_000492"
     CDS             22952..23377
                     /locus_tag="LGJ20_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HBK2384617.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVNFVAAWAILATVIC
                     VVAGGVGVYATEYALERYGRELPPESLAVKIVMSLFLQPVPWCRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRLEGREPCQS"
     gene            23365..23790
                     /locus_tag="LGJ20_000493"
     CDS             23365..23790
                     /locus_tag="LGJ20_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HBK2384618.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRANNVASGQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHHRPACTDASDCPQRLPESEPLPDAGQ"
     gene            23777..23935
                     /locus_tag="LGJ20_000494"
     CDS             23777..23935
                     /locus_tag="LGJ20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HBK2384619.1"
                     /translation="MPGSDPETNGDLSADIRQLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            23898..24365
                     /locus_tag="LGJ20_000495"
     CDS             23898..24365
                     /locus_tag="LGJ20_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384620.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTSDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            24358..24810
                     /locus_tag="LGJ20_000496"
     CDS             24358..24810
                     /locus_tag="LGJ20_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBK2384621.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHILASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            24877..25512
                     /locus_tag="LGJ20_000497"
     CDS             24877..25512
                     /locus_tag="LGJ20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBK2384622.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            25509..25856
                     /locus_tag="LGJ20_000498"
     CDS             25509..25856
                     /locus_tag="LGJ20_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HBK2384623.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            25861..26769
                     /locus_tag="LGJ20_000499"
     CDS             25861..26769
                     /locus_tag="LGJ20_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBK2384624.1"
                     /translation="MPIIDLNQLPAPDVVEELDFESILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGAVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            26762..27292
                     /locus_tag="LGJ20_000500"
     CDS             26762..27292
                     /locus_tag="LGJ20_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBK2384625.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAHFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            27303..29621
                     /locus_tag="LGJ20_000501"
     CDS             27303..29621
                     /locus_tag="LGJ20_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBK2384626.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATIARK
                     GLVQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTTRKGLVQLSSATNSDSE
                     TLAATLKAVKAAYDLANGKYTAQDATTARKGLVQLSSATNSDSETQAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGIKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDEMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTVWHETVQGGVVRWATGNTDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SRTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYSNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            29625..30152
                     /locus_tag="LGJ20_000502"
     CDS             29625..30152
                     /locus_tag="LGJ20_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2384627.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGGWMFKDGTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATEEERTRLEAWERYSVVV
                     SRVDPANPEWPEMPQ"
     gene            30724..31407
                     /locus_tag="LGJ20_000503"
     CDS             30724..31407
                     /locus_tag="LGJ20_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384628.1"
                     /translation="MTEWLNAHFGADAATYLSLLVGVLALFGIGGGVTYRIRQQNNIK
                     QKAKKTEGDVNQAGRDINQTTNVYHTSNESTEDPVKKKQDEHDLKIIDQILELLPYED
                     TTQWVEQSYLSGIRQDVCLNIEKAEKFSGEKYRLFNTEVNGAKDAFLDAMTAYVDSTL
                     PFMSVDYPNRKPVMLSLPHDWKSKSVESEANFRFHQQNVRETSGGMIARYEDFVRTFK
                     AHGFISDKL"
     gene            31762..32547
                     /locus_tag="LGJ20_000504"
     CDS             31762..32547
                     /locus_tag="LGJ20_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384629.1"
                     /translation="MYDYSFLLTPSTGFVKRLCGVWSNDFSINDDSDSDLGQTDIHLL
                     SSPHLNIISDPNELASKLKGLLMLMNGALSVLYGFERYQSFGPLSISGSEGINYSEIC
                     GFKSMDVTQVNPFNFHDIQQPHPRADNFSRLINLSSKHESLRVVLGMCALGDDWVNLY
                     RLWETIREYVYDKYVKGILNFPDTYKGKKYQRDKVISMAFNIDEKEISRFTGTANSFE
                     LLGYLARHGKGSSGGILKNPMSRVDASIFVHKATYKYCKLFLM"
     gene            complement(32709..33461)
                     /locus_tag="LGJ20_000505"
     CDS             complement(32709..33461)
                     /locus_tag="LGJ20_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384630.1"
                     /translation="MTKTNRHNFPPSVVGKVAERAAYICSNPSCHRVTIGPDQLAENL
                     SIKTGEAAHICAASPGGPRYDMSQSETERKSIKNAIWLCGACADLVDKNGGMGYPAEY
                     LRKWKRDHESLMKECLEGGKRLIFQFLSHRPDEKIALRVIHLLEDKSSLYLPYAQEDP
                     ARVSDSLKELRLSLTAIRSEIAPESPLSLIVESIVRACRHYMNSTPDRPGIEELNFSL
                     GAVRKVVGINIGELLKHYEIQVSHELHSIIPK"
     gene            33827..35017
                     /locus_tag="LGJ20_000506"
     CDS             33827..35017
                     /locus_tag="LGJ20_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HBK2384631.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGLVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRARI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            35030..35548
                     /locus_tag="LGJ20_000507"
     CDS             35030..35548
                     /locus_tag="LGJ20_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBK2384632.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            35605..35880
                     /locus_tag="LGJ20_000508"
     CDS             35605..35880
                     /locus_tag="LGJ20_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBK2384633.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            35913..36032
                     /locus_tag="LGJ20_000509"
     CDS             35913..36032
                     /locus_tag="LGJ20_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBK2384634.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            36025..38472
                     /locus_tag="LGJ20_000510"
     CDS             36025..38472
                     /locus_tag="LGJ20_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2384635.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAVVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQVKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDAAAIQAA
                     TPVTLNMALANQRSMEENAQLLLGTKASFQLSNDDVSHVGDVLSATMNKSAADFQGLS
                     DALTYLGPVARTAGVSLEQAAAMTGVLHDNNIRGSMAGTGSSAVVTRLQAPTGKAWDA
                     LKELGVKTSDKKGNMRPLFTILKEIQASFDKHKLGTSQKGEYLKTIFGEEALKSANVL
                     LAAAASGKLDKLTATLKASDGKTEELVKIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTNAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPETQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            38487..38966
                     /locus_tag="LGJ20_000511"
     CDS             38487..38966
                     /locus_tag="LGJ20_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384636.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSLATRFTGMTGAE"
     gene            38966..40129
                     /locus_tag="LGJ20_000512"
     CDS             38966..40129
                     /locus_tag="LGJ20_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBK2384637.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDNRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            40211..40429
                     /gene="ogrK"
                     /locus_tag="LGJ20_000513"
     CDS             40211..40429
                     /gene="ogrK"
                     /locus_tag="LGJ20_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HBK2384638.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(40702..42063)
                     /gene="yegQ"
                     /locus_tag="LGJ20_000514"
     CDS             complement(40702..42063)
                     /gene="yegQ"
                     /locus_tag="LGJ20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBK2384639.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(42166..42462)
                     /locus_tag="LGJ20_000515"
     CDS             complement(42166..42462)
                     /locus_tag="LGJ20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2384640.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            complement(42464..42760)
                     /locus_tag="LGJ20_000516"
     CDS             complement(42464..42760)
                     /locus_tag="LGJ20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HBK2384641.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            complement(42969..43301)
                     /locus_tag="LGJ20_000517"
     CDS             complement(42969..43301)
                     /locus_tag="LGJ20_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBK2384642.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(43492..44214)
                     /gene="baeR"
                     /locus_tag="LGJ20_000518"
     CDS             complement(43492..44214)
                     /gene="baeR"
                     /locus_tag="LGJ20_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBK2384643.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(44211..45614)
                     /gene="baeS"
                     /locus_tag="LGJ20_000519"
     CDS             complement(44211..45614)
                     /gene="baeS"
                     /locus_tag="LGJ20_000519"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBK2384644.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(45611..47026)
                     /locus_tag="LGJ20_000520"
     CDS             complement(45611..47026)
                     /locus_tag="LGJ20_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2384645.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVHVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(47027..50104)
                     /gene="mdtC"
                     /locus_tag="LGJ20_000521"
     CDS             complement(47027..50104)
                     /gene="mdtC"
                     /locus_tag="LGJ20_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBK2384646.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVVVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(50105..53227)
                     /gene="mdtB"
                     /locus_tag="LGJ20_000522"
     CDS             complement(50105..53227)
                     /gene="mdtB"
                     /locus_tag="LGJ20_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBK2384647.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGFAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPELMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(53227..54474)
                     /gene="mdtA"
                     /locus_tag="LGJ20_000523"
     CDS             complement(53227..54474)
                     /gene="mdtA"
                     /locus_tag="LGJ20_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBK2384648.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            54753..54809
                     /locus_tag="LGJ20_000524"
     CDS             54753..54809
                     /locus_tag="LGJ20_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBK2384649.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            55081..55137
                     /locus_tag="LGJ20_000525"
     CDS             55081..55137
                     /locus_tag="LGJ20_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBK2384650.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            55410..55469
                     /gene="ibsA"
                     /locus_tag="LGJ20_000526"
     CDS             55410..55469
                     /gene="ibsA"
                     /locus_tag="LGJ20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HBK2384651.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            55690..56349
                     /locus_tag="LGJ20_000527"
     CDS             55690..56349
                     /locus_tag="LGJ20_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK2384652.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            56346..57107
                     /locus_tag="LGJ20_000528"
     CDS             56346..57107
                     /locus_tag="LGJ20_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBK2384653.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVCQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            57104..59044
                     /gene="yegI"
                     /locus_tag="LGJ20_000529"
     CDS             57104..59044
                     /gene="yegI"
                     /locus_tag="LGJ20_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBK2384654.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(59056..60408)
                     /gene="yegD"
                     /locus_tag="LGJ20_000530"
     CDS             complement(59056..60408)
                     /gene="yegD"
                     /locus_tag="LGJ20_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2384655.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            60542..61399
                     /gene="alkA"
                     /locus_tag="LGJ20_000531"
     CDS             60542..61399
                     /gene="alkA"
                     /locus_tag="LGJ20_000531"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBK2384656.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(61438..64755)
                     /locus_tag="LGJ20_000532"
     CDS             complement(61438..64755)
                     /locus_tag="LGJ20_000532"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBK2384657.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            65073..65714
                     /gene="udk"
                     /locus_tag="LGJ20_000533"
     CDS             65073..65714
                     /gene="udk"
                     /locus_tag="LGJ20_000533"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK2384658.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            65806..66387
                     /gene="dcd"
                     /locus_tag="LGJ20_000534"
     CDS             65806..66387
                     /gene="dcd"
                     /locus_tag="LGJ20_000534"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBK2384659.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            66409..68262
                     /gene="asmA"
                     /locus_tag="LGJ20_000535"
     CDS             66409..68262
                     /gene="asmA"
                     /locus_tag="LGJ20_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBK2384660.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFMADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(68536..70119)
                     /locus_tag="LGJ20_000536"
     CDS             complement(68536..70119)
                     /locus_tag="LGJ20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBK2384661.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            70778..71917
                     /gene="wza"
                     /locus_tag="LGJ20_000537"
     CDS             70778..71917
                     /gene="wza"
                     /locus_tag="LGJ20_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBK2384662.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            71923..72366
                     /gene="wzb"
                     /locus_tag="LGJ20_000538"
     CDS             71923..72366
                     /gene="wzb"
                     /locus_tag="LGJ20_000538"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBK2384663.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            72369..74531
                     /gene="wzc"
                     /locus_tag="LGJ20_000539"
     CDS             72369..74531
                     /gene="wzc"
                     /locus_tag="LGJ20_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBK2384664.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIALSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTITKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLREVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            74624..75463
                     /gene="wcaA"
                     /locus_tag="LGJ20_000540"
     CDS             74624..75463
                     /gene="wcaA"
                     /locus_tag="LGJ20_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBK2384665.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVSALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            75466..75954
                     /gene="wcaB"
                     /locus_tag="LGJ20_000541"
     CDS             75466..75954
                     /gene="wcaB"
                     /locus_tag="LGJ20_000541"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBK2384666.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            75951..77168
                     /gene="wcaC"
                     /locus_tag="LGJ20_000542"
     CDS             75951..77168
                     /gene="wcaC"
                     /locus_tag="LGJ20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBK2384667.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNLNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGSGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            77143..78360
                     /gene="wcaD"
                     /locus_tag="LGJ20_000543"
     CDS             77143..78360
                     /gene="wcaD"
                     /locus_tag="LGJ20_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBK2384668.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMIINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            78371..79117
                     /gene="wcaE"
                     /locus_tag="LGJ20_000544"
     CDS             78371..79117
                     /gene="wcaE"
                     /locus_tag="LGJ20_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBK2384669.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGLWAELSWHLRQRTTSKTKALYNKV"
     gene            79133..79681
                     /gene="wcaF"
                     /locus_tag="LGJ20_000545"
     CDS             79133..79681
                     /gene="wcaF"
                     /locus_tag="LGJ20_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBK2384670.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            79707..80828
                     /gene="gmd"
                     /locus_tag="LGJ20_000546"
     CDS             79707..80828
                     /gene="gmd"
                     /locus_tag="LGJ20_000546"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBK2384671.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGRGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            80831..81796
                     /gene="fcl"
                     /locus_tag="LGJ20_000547"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             80831..81796
                     /gene="fcl"
                     /locus_tag="LGJ20_000547"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBK2384672.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            81799..82278
                     /gene="gmm"
                     /locus_tag="LGJ20_000548"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             81799..82278
                     /gene="gmm"
                     /locus_tag="LGJ20_000548"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBK2384673.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVSEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            82275..83498
                     /gene="wcaI"
                     /locus_tag="LGJ20_000549"
     CDS             82275..83498
                     /gene="wcaI"
                     /locus_tag="LGJ20_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBK2384674.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLVAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHIADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            83501..84937
                     /gene="cpsB"
                     /locus_tag="LGJ20_000550"
     CDS             83501..84937
                     /gene="cpsB"
                     /locus_tag="LGJ20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBK2384675.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLKGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISTHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            85218..86588
                     /gene="cpsG"
                     /locus_tag="LGJ20_000551"
                     /gene_synonym="manB"
     CDS             85218..86588
                     /gene="cpsG"
                     /locus_tag="LGJ20_000551"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBK2384676.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANNFPPVDETKRGRYQQINLRDAYVAHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREVLAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            86724..88118
                     /gene="wcaJ"
                     /locus_tag="LGJ20_000552"
     CDS             86724..88118
                     /gene="wcaJ"
                     /locus_tag="LGJ20_000552"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBK2384677.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            88120..89598
                     /gene="wzxC"
                     /locus_tag="LGJ20_000553"
     CDS             88120..89598
                     /gene="wzxC"
                     /locus_tag="LGJ20_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBK2384678.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCMVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMEGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            89874..>91132
                     /gene="wcaK"
                     /locus_tag="LGJ20_000554"
     CDS             89874..>91132
                     /gene="wcaK"
                     /locus_tag="LGJ20_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBK2384679.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALIMRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMSIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVL"
BASE COUNT        22256 a        22750 c        24580 g        21546 t
ORIGIN      
        1 ggcgatggaa aaatccgtcg agctggtaaa ggaatatgtt cgtgccggct tcagtaaaat
       61 tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac cagaaacggt
      121 tgcggaacga gctgctgtgc tttgctttgc tgcggaaagt gtggcgacag attgccagcg
      181 tgagcaactg agctatgtca ttggcaccga agttccggtt ccgggcggtg aggccagcgc
      241 cattcagtca gtacacatca cccatgttga agataccgcc aatactttac gtacgcatca
      301 aaaggccttt attgcccgtg ggctgacaga ggcgttaaca cgcgtgattg ccatcgtggt
      361 gcagccgggc gtggaatttg atcacagcaa tattatccat tatcagccgc aggaagcgca
      421 gccgctggcg caatggatag aaaacacccg aatggtttat gaagcacatt ctaccgatta
      481 ccagacccgg acggcttatt gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg
      541 tcccgcatta acctttgctt tacgcgaggc gatatttgca ctggcacaaa ttgagcagga
      601 acttatcgcc cctgaaaatc gcagcggttg cctggcggta attgaagaag tgatgctcga
      661 cgaaccgcaa tactggaaaa aatattatcg tacgggtttt aacgattcat tactggatat
      721 tcgttacagc ctgtcggatc gtattcgtta ttactggccg catagtcgga ttaaaaatag
      781 cgtcgaaacg atgatggtga atcttgaagg cgtggacatc ccactgggca tgattagtca
      841 gtatcttccc aaacaatttg aacgcattca gtccggggaa ttatcagcaa taccgcatca
      901 gctgattatg gataaaattt atgatgtttt gcgcgcctat cgctacggct gtgcggaata
      961 aggacggtat atgactaacc tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga
     1021 agttttaacc catatcggta atgagatgct cgccaaaggt gtggttcatg atacctggcc
     1081 acaggcatta attgccagag aagcagaatt ccctaccggg ataatgcttg agcagcacgc
     1141 cattgcaata ccgcattgtg aggcgattca tgctaagtcg tcagccattt atctgttaag
     1201 gccaacaaat aaagttcatt ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt
     1261 ggttattgcg ttgattgtgg aaaatccgca gcagcaattg aaacttttac gctgtttatt
     1321 tggcaagtta caacagcccg atatcgtcga gacactaatc actcttcctg aaacccagtt
     1381 aaaggaatac ttcacaaagt atgttttaga ttcagacgaa taaatccctc tgtaacaata
     1441 aaaaggacta tttatgaaac gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc
     1501 tacgatggcg gcggaagaaa ttaaagagtt gtgtcagaat cataatattc ctgttgaatt
     1561 aatccagtgt cgggttaatg aaatagaaac ctatatggat ggtgtgcatt tgatatgcac
     1621 cactgccaaa gtggatcgta gttttggcga tattccgtta gttcacggca tgccttttat
     1681 ttctggtgtc ggtatcgaag cattacaaaa taaaattctg actatcttac aggggtgacc
     1741 tatgttttca gaagtcatgc gttatattct cgacctcggc cctacagtga tgctgccggt
     1801 tgtcatcatt attttttcta aaatattagg catgaaggca ggcgattgct ttaaagcggg
     1861 tctgcatatc gggattggct ttgttggcat tggccttgtg attggcttaa tgctggattc
     1921 cattggtccg gcggcgaaag cgatggcgga aaatttcgac ctgaatctgc atgtggtcga
     1981 tgtcggctgg ccgggctctt caccaatgac ctgggcgtcg caaattgcgc tggtggcgat
     2041 tccgattgcg atattggtta acgtggcgat gttactgacc cgtatgacgc gggtggtaaa
     2101 tgttgatatc tggaatatct ggcatatgac cttcaccggc gcgttgctgc acctggcaac
     2161 cggttcatgg atgataggga tggcaggtgt ggtaattcac gcggcgtttg tttataagct
     2221 cggcgactgg tttgcccgcg atacccgaaa tttctttgag ctggaaggta ttgctattcc
     2281 gcacggtacg tcggcgtata tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa
     2341 aatcccaggc gttaaccgaa ttaaatttag cgccgacgat attcagcgca aatttggtcc
     2401 atttggcgag cctgtcaccg tgggttttgt gatggggctg attatcggca tcctcgcggg
     2461 ttacgatgtc aaaggtgtat tgcagctggc ggtaaaaacg gcggcagtga tgctgctaat
     2521 gccacgggtg attaaaccca tcatggatgg tttaacgccc atcgctaagc aggctcgtag
     2581 tcgtttacag gcgaagttcg gcggtcagga gttcctgatt ggccttgatc cggcgttgct
     2641 gctgggacat acggcggtgg tatcggcaag cctgattttt atcccactca ccattttaat
     2701 tgctgtttgt gtgccgggta atcaggtgct gccgtttggc gatcttgcca ccatcggctt
     2761 cttcgtggcg atggcggtcg ccgtgcatcg tggaaatctg ttccgcacct taatctcggg
     2821 tgtcatcatt atgagcatca ccctgtggat cgcgacgcaa actattggtt tgcacaccca
     2881 actggcggct aatgctgggg cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg
     2941 cggttctccc attacctggt tactgattca ggttttctcc ccgcaaaata ttcccggttt
     3001 cattattatc ggtgcaattt atctgaccgg tattttcatg acctggcgta gagcgcgtgg
     3061 ctttattaaa caagagaaag tcgttctcgc agaataattt ttacctgagg gggagtttat
     3121 ccccccttaa caatcaggag tttttatgaa atcagtggtg aatgatactg atggtatcgt
     3181 gcgcgttgca gaaagcgtca ttcctgaaat taaacatcag gatgaggtac gggtaaaaat
     3241 tgccagctcg ggcttatgtg gttccgattt acccaggata tttaaaaatg gtgcacatta
     3301 ttatccaata acgttaggcc atgaatttag cggctatatt gatgcggtgg gatccggtgt
     3361 tgatgattta caccctggcg atgcggttgc ctgtgtgccg ttattaccct gttttacttg
     3421 tccagagtgt ttgaaagggt tttattccca gtgcgcaaaa tatgatttta ttggctcgcg
     3481 gcgtgatggt ggatttgctg aatatattgt cgttaagcga aaaaatgtct ttgctctacc
     3541 cacggatatg cctattgagg atggggcttt tattgagccg attaccgttg gactgcatgc
     3601 ttttcattta gcgcaaggtt gtgagaataa aaacgttatt attattggtg ccggaaccat
     3661 tggcctgctg gctattcagt gcgctgtcgc gctgggagca aagagtgtga cggctatcga
     3721 cattagctca gaaaaactgg cactggcaaa atctttcggt gcgatgcaaa catttaacag
     3781 tagcgaaatg agcgcgccgc aaatgcagag cgttttacgc gaactgcgct ttaatcagct
     3841 tatcctcgag acggctggcg taccgcaaac tgtcgaactg gcggtagaga ttgccggtcc
     3901 tcatgcccaa ctggcgctgg tgggcacgtt gcatcaggat ctgcatttaa catcggcaac
     3961 gtttggcaaa atattgcgta aagagctgac ggttatcggc agttggatga actactccag
     4021 cccttggccg gggcaggagt gggaaacggc gagccggttg ctgacagaac gtaagttaag
     4081 cctggagcca ttaatcgctc accgtggaag ctttgaaagc ttcgcccagg cggtgcgtga
     4141 catcgctcgt aatgccatgc cgggcaaagt gttgctcatt ccctgaaacc gcgggccagc
     4201 gtgatgctgg cccggtattg tgcaaaacag atcattcacc aatggtcccc cttcgtttac
     4261 actagctaca attgaatatg ggtaaatgac gatgaattca ttcgagcgaa ggaataagat
     4321 catccaatta gtgaatgaac agggaaccgt gcttgttcag gatctggcgg gagtatttgc
     4381 tgcctcggaa gcgacaatcc gtgccgattt gcgctttctc gaacaaaaag gcgtggttac
     4441 gcgctttcat ggcggtgcgg cgaaaataat gtccggtaat agtgaaaccg agacccagga
     4501 agtcgggttt aaagagcgat ttcagctcgc cagcgcgcca aaaaacagaa tagcgcaggc
     4561 ggcagtcaaa atgatccatg aagggatgac cgttattctc gacagcggaa gtaccacaat
     4621 gcttatcgcc gaaggattaa tgaccgctaa aaatatcacg gtgattacca acagtctccc
     4681 ggcagcgttt gccctttccg aaaataaaga cattactctg gtcgtctgtg gtggcacggt
     4741 ccgccataaa acgcgctcga tgcatggttc cattgccgag cgttcattgc aggatattaa
     4801 tgccgattta atgtttgtcg gtgctgatgg tattgatgcg gttaatggta ttacgacctt
     4861 taatgagggt tattccgtta gcggtgcgat ggtcacagcc gctaataaag tgattgccgt
     4921 tctcgattca tcgaaattta accgtcgcgg ttttaatcag gtactgccaa tcgaaaaaat
     4981 tgacattatt attaccgatg atgctgtatc agaggtggat aaactggcat tgcagaagac
     5041 acgggtgaaa ttaattacgg tgtagtattg ttctgtatgc cggataaggt atttacaccg
     5101 catccggcat tacggattct ccctggttca agagagaata gtttgattaa cgcaataatg
     5161 gacaatctgg tggtaatcga caacgcaacg ccgccggaag tatttcaata tgaaagtttt
     5221 gtccgctcaa cggttcgcca tcaagattaa aagtgatttc gtgtggggct tgtatatcaa
     5281 accacgacga agcgccttcg ataatattcg ggttatcttc gtcagatttt aaggttgata
     5341 cgagagccgg aagaatttca tcgccggtaa aaatgcgcag ttgcagcaag ccatcgttaa
     5401 ttaacgcgtt cggacacaat tgctgaccgc caccggcctg acgcccgtta ccaataccaa
     5461 tgaccagggc gtcaccttgc cagtgaaagt tttcaccgcg gatttcacaa cggtccggtt
     5521 gcagagtatc catgcgcatt aagccatgaa tgatgtaaga gacgccaccc agcgcggctt
     5581 ttaatttttc cggcgtttct gtggtaatac gcgtcccaaa tccgcctgtc gccatattaa
     5641 taaaacaggt ttgtttgttg acctgcgcca tatctatcgc aatggcgtta ccggcaattg
     5701 ccagtttcag cgccttatcc agtgcctcag gaatccctac actggtggca aaatcattgg
     5761 cggttcctaa tggcaaaatt cccagcgcgg gtatgtcatc cccctcacac tgaatcaacg
     5821 ccgtagaaac ttcattaatg gtgccatcgc caccaccggc aatcaccgtt gcgacgccca
     5881 acttccgggc ctcctctaca tatcgtgcgg catcgccttt ctcccaggtg acccgcacat
     5941 ggatcgtcat tccttcctca cgcaacagca taattgcttc gcgcaagggt agattgtcag
     6001 tacttttgcc attaagaatc agtaagctgg cgggaaattc tgccatgatc gtatgtgcct
     6061 ttatgattgg tctgtagata gtgtagagca gaaaacaaaa ggtggagtca gaacaggatg
     6121 aaagtcggag gataaaaagt cagttcaagt atggaggatt cagtggttac caccaggagc
     6181 ttgcaatggg gacagcgaat actaacggct gccaccgata aatttcaaaa aagagcatat
     6241 gcctaatatt caaccaaaca gtggcatctt aaatatacta ttccaaatat attaaagccc
     6301 ccatggagtt accctgaagg gcctcaatgt ccgtaattcc tacttatgta ggaaatgttg
     6361 tacagaacat ttattataat cctattcaat tataataatc atgccattat tatatttaaa
     6421 cactagagag tgtcgttggt atttaatggg ggaaggtgag atgaaaaaga tagctgctat
     6481 atcattaatt agtattttta ttatgtctgg ttgtgctgtg cataatgatg agacaagtat
     6541 cggtaaattt ggtcttgcat ataaaagtaa tattcagcgt aaactcgata accaatacta
     6601 caccgaagcc gaagcttctt tagccagggg cagaatatct ggtgcagaaa atatagtaaa
     6661 aaatgatgca gcccatttct gtgttactca gggcaaaaaa atgcagatag ttgacctgaa
     6721 gacagaaggt gcaggattac atggcgtcgc tcgtctgaca ttcaaatgtg gagagtgaga
     6781 atattttttg gtaagcgtca aacatgcgcg ttctggttgt gcttagccgg aacctgtgcg
     6841 agcacgatgc cgttacgtga aaggcatcgt gctatgaagg gagattctat cgatgtggtc
     6901 aatggaagac ggtgaccagg gatagggctt atgcataaaa aataagcccg tgtaagggag
     6961 atttagggtg tcaccagtag gggctttcaa cggtactatg cgggtttgag cggcataaat
     7021 taccactgaa agcccttaaa cgttactcta ctgtggacac tgtgtggaca ctctcggcct
     7081 cagtaccacc tcttagcgga ttaagagaaa tggcgtcctg aaggtactct ggcgcaaaat
     7141 gagcgtaaac catagtttgc tcaatccgcg tgtgacctag tatccgttgt agcgtgataa
     7201 tacttcctcc attaatcatg aaatgagtgg caaagctgtg ccttagtgca tgtgtggctt
     7261 gccccgttgg caaatccggt tttattgctt tcattgttcg tctgaagcga gggtaatcag
     7321 catcagggaa taaaaaacct cgtttgttat ccgcgatcat tttggcaaca gcctctgaga
     7381 tcgggacggt gcgtggtttg tttgttttcg ttttaacaaa cgtgacgcgg ttatggatga
     7441 tattttctgc tttcaaacga gctgcttctc cccaacgtgc tcctgtactc aggcaaagaa
     7501 tcgcaatctt tttattgtcg ccgtcaagtg ctgcaagcag taaggcaatt tcttcctgtg
     7561 tgagatagcc tgtttctggt ttttcctcct taagcctctt tgtccctctg atagggtgct
     7621 caccaaagaa taactccgct tcaatcaggg ctgtaaacat gccgctaata catgttaaat
     7681 cacgattgat actcgaaggt ttaataccct gacttcttcg ggtggcgcag tactggctga
     7741 taagggattt cgtgatttga aatgcgcatg ggtcattcgt tatttttgta aagatttcaa
     7801 tttttccaag attagatttc ccatgctctt cgtgtttacc ctttaaatcc caccagatct
     7861 gtgtcagttc cgacagacgt cgtttgtctg ttggttttga tagccattct ttattgtggt
     7921 ggttgtacaa cgtgtatttt tcgaaagcga cagcttcgct tttcttatca aacttcctac
     7981 ggatgcgttt tccattacgt ccagtagggc ggatgtccac ttcatatcga ccatcatcga
     8041 gttttttgat tgccatcaga aaaccctccg agtggtactt ttttttgcta ctactaatcg
     8101 cttttttcgt ggtggctgaa atttagccac caatagtagg cacttgtgat gaatatattc
     8161 acgataaatt gttaaccagt cttttgaccg gagtggggcg acgttgtttc gttttgccca
     8221 aagtgtgcga gagcgggcgc aatttgcccg gcttctggag ctacctgatc agtcatgaac
     8281 cacaaagtat atttagtaaa tctaggatgt tgtaagacct tcattatggc ttcaactcca
     8341 gcgttttttg accggctctc atagctcgaa agtgagctgt aggctacacc agttaattca
     8401 ctgaattctt tacggtttaa cctttcagat tcacggatta gcttcaactt ctccgaaacg
     8461 tctattgaca taattactcc gattgcgtaa tttcttgctg atagtgtgaa atgttgtgct
     8521 tctggagtta tccttttagg caataattag ccattaggag ccattagaag cactaaggga
     8581 gaatcgtagc agatgaatag acagcttgta agcgtgactg atgccgtgcc ttatcaggag
     8641 tttgcaaaac tcattggtaa aactccaaga gctgtaaggg gcatgattga gaaagggaaa
     8701 ttaccagtta ttgagattac tgaccctcag tcagtatcgg ggcgtgctgg tgaatattgg
     8761 gtataccttc cggcatggaa taacggacta aaactggctt atgaaagccg tcctaaagag
     8821 attcgtgacg gctggttgat gtggttaggt ctcggtgaac cacgttaagg agaaccgtat
     8881 gaatgagcct cgttgtattg ctcagttatt gcgtaacgaa agccccaggg cgattgactt
     8941 caccatcacc catggtaagg ggcgtaaggg aatcattatc cgcaccaaaa aacagagtcc
     9001 gttaaaaaag gctctgacct ttctgaaaag ccggagggtc tggaaatgac tgtgatgacg
     9061 ctcaatctcg ttgaaaaaca gccagcagct atgcgccgga taattggtaa gcatctggcc
     9121 gtccctcgct ggcaggagac atgcgattat tataatcaga tgatggaacg cgaacggcta
     9181 acggtttgct tccatgcgca gttaaaacag cgtcacgcaa cgatgcgttt tgaagaaatg
     9241 aacgacgtcg aacgtgaacg actggtttgt gcaattgatg aattgcgtgg cgcattctca
     9301 aaacgccgtc aggttggtgc aagtgagtat gcatatatta gttttttaac agtcagtcag
     9361 cgtcgcactt tatttatgca cgcacgactg actgaaaaag aattcaacca gccatactgg
     9421 cgaattaatg aagaatcatg ttactggcgt gatgctttgt tccgtgcatt acgtgaatta
     9481 ttcagcctgt ttgagtatgc accgacaatt ctgacgtcgg taaaaccaga gcaatatctg
     9541 cattaaataa ttaaccagag tttttaacgc acttaatcgt gcggggcttc tttttgcctg
     9601 gagaaagtca tgcatacagt ttctgaaaat cagtgcggta tatacgcatt actgctgcaa
     9661 caggccagaa ccgaagcaca ggccgacgct gcgacgcgct tttcttctca tcttgacgcc
     9721 atgattcgcc acatcacaaa ggcggagtta tcccgcgtgg agatagtcga gctgctcagt
     9781 caggagtcgg aaaaatttca caatatcgga ttgtctcgcg ggaaggtgct ttgatgtcct
     9841 gttctcattc agttgtatta ctgaataacg ccttaaaaat cgccgttatg aaaaatggcg
     9901 atttgtctct tattcaactt tgtcttgata aagaaaaacg cgacataact gaatctgtta
     9961 tcgcgattta tcagaatgaa ttaaacctcc tgtctgatgt ggtcaattta cttgttaaac
    10021 gcgctgtatt ccacaagcaa atttcatccg tggatgagct gacaaaatta acgacagaaa
    10081 tcgccagcta ttgcgctgat gaattcaaga aactgaacga taaaaggagc tggtaatgcc
    10141 ggacaacgta gattttattc aggaacaaca ggctgaatta ctggagcgcc agattaacgc
    10201 ggcaagggta aaacattgcg gtgtttcttc gctggtttgc gaagagtgtg atgcgccaat
    10261 acctgctgcc cgtcgtgcgg cttatccgtc agccacgcgt tgtgtttcct gtcagtcagt
    10321 ctttgaagca aaaaacaaac attaccggag aacggcatga gtattcgtat tgaaattggc
    10381 gaacgttatg tcgttaccag tgacagcttt cagtttattc tccacgagaa aaagagagcg
    10441 gaaagcggta aaaacgccgg tcaggaatgg ctgtcggtgg ttggttatta cccgaaatta
    10501 agccagctcg tttccggcct gatgcatcac gatattctga ccggaagcgc aaagtctttt
    10561 gctgatttaa acgcgcaggt tgagcaactc agcaagcgtt gttcagaggt ttttggctca
    10621 tatggcagtt aaagcctccg ggcgttttgt ccctccgtca gcatttgccg caggcaccgg
    10681 taagacgttt accggtgctt atgcatggaa cgcgccacgc gaggccgtcg ggcgcgaaag
    10741 accccttaca cgtgacgaga tgcgtcaggt gcaaggtgtt ttatccacga ttaaccgcct
    10801 gccttacttt ttgcgctcgc tgtttacttc acgctatgac tacatccggc gcaataaaag
    10861 cccggtgcac gggttttatt tcctcacatc cacttttcag cgtcgtttat ggccgcgcat
    10921 tgagcgtgtg aatcagcgcc atgaaatgaa cactgacgcg tcgttactgt ttctggcaga
    10981 gcgtgaccac tatgcgcgcc tgccgggaat gaatgacaag gagctgaaaa agtttgctgc
    11041 ccgtatctca tcgcagcttt tcatgatgta tgaggaactc agcgatgcct gggtggatgc
    11101 gcatggcgaa aaagaatcgc tgtttacgga tgaggcgcag gctcacctgt atggtcatgt
    11161 tgctggcgct gcacgtgctt tcaatatttc ccctctctac tggaaaaaat accgtaaagg
    11221 gcagatgacc acgaggcagg catattctgc cattgcccgt ctgtttaacg atgagtggtg
    11281 gactcatcag cttaaaggcc agcgtatgcg ctggcatgag gcgttactga tagctgtcgg
    11341 ggaagtcaat aaagaccgtt ctccttatgc cagtaaacat gccattcgtg atgtgcgtgc
    11401 acgccgccag gcaaatctgg aatttcttaa atcgtgtgac cttgaaaaca gggaaaccgg
    11461 cgagcgcatc gaccttatca gtaaggtgat gggcagtatt tctaatcctg aaattcgccg
    11521 gatggagctg atgaacacca ttgccggtat tgagcgttac gccgctgcag agggtgatgt
    11581 ggggatgttt atcacgctga ccgcgccgtc aaagtatcac ccgacacgtc aggtcggaaa
    11641 aggcgaaagt aaaaccgtgc agcttaatca cggctggaac gatgaggcat ttaatccaaa
    11701 ggatgcgcag cgttatctct gccgtatctg gagcctgatg cgcacggcat tcaaggataa
    11761 tgatttacag gtctacggtt tgcgagtcgt cgagccacac cacgacggaa cgccgcactg
    11821 gcatatgatg cttttttgta atccacgcca gcgtaaccag attatcgaaa tcatgcgtcg
    11881 ctatgcgctc aaagaggatg gcgacgaaag aggagccgcg cgaaaccgtt ttcaggcaaa
    11941 acatcttaac cggggcggtg ctgcgggata tattgctaaa tacatctcaa aaaatatcga
    12001 cggctatgca ctggatggtc agctcgataa cgataccggc aggccgctga aagacactgc
    12061 agcggctgtt accgcatggg cgtcaacgtg gcgcatccca caatttaaaa cggttggtct
    12121 gccgacaatg ggggcttacc gtgaactacg caaattgcct cgcggcgtca gcattgctga
    12181 tgagtttgac gagcgcgtcg aggctgcacg cgccgccgca gacagtggtg attttgcgct
    12241 gtatatcagc gcgcagggtg gggcaaatgt tccgcgcgat tgccagactg tcagggtcgc
    12301 ccgtagcccg tcggatgacg ttaacgagta cgaggaagaa gtcgagagag tggtcggcat
    12361 ttacgcgccg catctcggcg cgcgtcatat tcatatcacc agaacgacgg actggcgcat
    12421 tgttccgaaa gtgccggtcg ttgagccttt gactttaaaa agcggcatcg ccgcgcctcg
    12481 gagtcctgtc aataactgtg gaaagctcac cggtggtgat acttcgttac cagctcccac
    12541 accttctgag cacgccgcag cagtgcttaa tctggtagat gacggtgtta tcgaatggaa
    12601 tgacccggag gtcgtgaggg cgctcagagg tgcattaaaa cacggcctga gaagaccaaa
    12661 ccgtcagcaa agaaacggaa gcccgttaaa accgcatgaa atagcgccat cggccagact
    12721 gacccggtct gaacgattgc aaatcacccg tatccgcgtt gaccttgctc agaacggtat
    12781 caggccgcag cgatgggagc ttgaggcgct ggcgcgtggc gcgaccgtaa attatgacgg
    12841 gaaaaaattc acgtatccgg tcgctgatga gtggtcggga ttctggcatc aatttgagta
    12901 aggatctcat ctatacagta taagtagttt tgactgtaat tgattgtttt ttatatggtt
    12961 gcatgcatta aatctacgtc aatatcatta tgttataatg cattacttaa aaatgttgga
    13021 gataaaaatg ttaaagcctg cagatgttgt ttgtttgaaa agcggtggat ttaaaatgac
    13081 tgtatctgac tctactgaaa ctcatgctaa atgtatttgg catgataaag aaggagtttt
    13141 gcacgaggag tcattctcca ttgatttact ggaaatagca gagagtaaaa gtggtatgct
    13201 ttgtcgtcca cgtaagtgaa tcataataaa attcacgggg gaaagtatga aacaatacag
    13261 cgaattagaa aacaatgtta agagattcat tgttgagcat gaaaaaggga tttctattga
    13321 tgatattcat cataagttcc ggatgaagga tggtcagaat cgcaagatgg cagattatct
    13381 tattgataat aaaaagataa ttttggagat gaaaagtctt ttttctgaca gggtcaagaa
    13441 tgtcaatgat aaactgaatg agttggttaa aactgatagt tggcttgcaa aaaactggca
    13501 tggtgctatt catttagaag agttgataaa aagacaccca gactcaaagc gatttagaaa
    13561 tgatataatg aactttgctt atgaaaatat taaaacaaag atagttaagg aagctaataa
    13621 gcaaataaat gcaacaaagg atgttttgga tttaaatgac tcgattggcg ggttgatttt
    13681 gttaaatgat aatgtctttt cacatgagtc taattatttg tcagatgaaa tattatattt
    13741 gctaggcaca aagagatatt cgagtgttga gtttgtggtt tatattgcaa aattgattga
    13801 taaacaggtt gatgtttgtg tgctacttga cagtcaatct aaaaataaaa attatattga
    13861 atggtatatg aataatgttt ttttattgaa ctgggcgtct tttaataaat atgcaataaa
    13921 gatgtagtgg tgtggatgga tatgaaatga gtcaaccaag atgcaccttg gtcagtaatc
    13981 tcatgtgaga taccacatat aacaccaaaa gtgtcaggtt gatggccatt gttcaccgtt
    14041 tacagcagat tatggtcaac gaaaatctga cgcctgacga gctggtcggg tgtgccgaaa
    14101 tagtctggga taattacggg cggtttaact atatcggtca gtccagagtt gcgccaccac
    14161 cacgcagacg atagagaacg ccgccagtcg tgaaacttgt tttcaggctg gcggggttga
    14221 acaacgagcg aagtgaggcg ttagcattaa gttaatttta atgaatggta ctctatcgag
    14281 ctgattgtag atgcgatata tttacgatca tttacagaga gtattgccgc tagtagacga
    14341 cataccagag cctgcatttg tagcaatata tcgctaccat attgacaatc attgatacta
    14401 gagaaaccaa gcggttgccc attaaaaatg gcttcatgaa aattatcatt gcgtataact
    14461 gaaacattac attcatctgt tgcccatgac gggattttta caccgtaatt ttcgcacatc
    14521 caatatactc tattttgatg agatggtttt ttctctttaa tgactttatt cctttcatcc
    14581 catgctatag caaaacagca atcaagagcc atataaagat actggaattt ctcaaaggaa
    14641 aggtatagtg ggttatgtga caggaatagg gagtgtatca ccgcagctat tcttttaatg
    14701 gagcgatcat ctttttgttt gtcttttata tagttaagag ctagctctat tacttctttt
    14761 tcagagcatc caacgagaat aaagtcggtt aacttagtgg gcttgatagt ggttgcatca
    14821 agaaaaccag cgtcagtggt tgtcaatctt atacccttga aaaaagagag gcaccacaca
    14881 acaaagttga gagtttctaa cgaggatgtg ttttttaatt ttagtgtgtg cgttttgggc
    14941 atgccgaata ctcggtaact ataaggcatt gtgcatgtgt caccattaag gttatatacc
    15001 tttcgattcc cagggtatat ccaaccatta acaacgtttt tatcattgtt aatacgttcc
    15061 acgttctcaa taagaccggg taaagtacgc aaagaaaatt gttcagtctc aatgttaact
    15121 tctactggat agtatccaaa ctccgaaata atctcatttg gttgctgttc gtcaacttca
    15181 ccttgcataa ctttctccgt tcaaaaacag gcacgataat tctcgtaagc ctatcatgtt
    15241 cgcaaagtat ccatacctgt ggattttgta aatttaagtt gatttgtcat taaataagcc
    15301 gttgcatgca acaagtgaat gtttttgcat gcgtcgggga tgtccgttca ggctgcgtgc
    15361 ggtcaggact ggtgcggatc catagtatct atgcaactgc attaaaaccg ccccatgaag
    15421 cgggcgggcg aggcggggaa agcactgcgc gctggcggtg gtgctgattt tattttttca
    15481 gcgtctcagc gtctcagcgt ctcagcgcgt cgtgacggcg tttggattgt gcgccggggc
    15541 gttggtgtgt ctgcggggtg ttttgtgcgg tggtgagcgt gtgagggcgt gatgacgggg
    15601 tgtaaaaaag ccgcccgcag gcggcgatgt tcagccgttg tcagtgtcca gtgagtagtt
    15661 tttaaagcgg atgacctcct gaccgagcca gccgtttatc tcgcagatcc tgtcctgtaa
    15721 cgggataagt tcattgcgga caaagacctt tgccactttc tcaatatcac ccagcgaccc
    15781 gacgttctcc ggcttgccgc ccatcaactg aaaggggatg cggtgcgcgt ccagcaggtc
    15841 agcggcgctg gcttttttga tattaaaaaa atcgtccttc gttgccactt cactgagcgg
    15901 gataatttta atgccgtcag ctttcccctg cggggcatag agaaacaggt ttttaaagtt
    15961 gttgcggcct ttcgacttca ccatgttttc gcgaagcatt tcgatatcgt tgcgatcctg
    16021 cacggcatca gtgacgtaca tgatatatcc ggcatgagcg ccgttttcgt aatacttgcg
    16081 gcggaacagc gtggccgact cattcagcca ggcagagtta agggcgctga gatattccgg
    16141 cagaccgtac agctcctgat taatatccgg ctccagcagg tgaaacacgg agccgggcgc
    16201 gaaaggtgtc ggctcgttga aggacggcac ccaccagtaa acatcctcct ccacgccacg
    16261 gcgggtatat tttgccggtg aggtttccag tctgatgacc ttaccggtgg tgctgtaacg
    16321 cttttccaga aacgcattac cgaataccag aaaatccagc acaaagcggc tgaaatcctg
    16381 ctgagaaagc cacgggtgtg ggataaacgt tgaggccaga atattacgtt tgacgtaaat
    16441 cggtgagctg tgatgcacgg cagcacgcag gctttttgcc agaccggtaa agctgaccgg
    16501 tggctcatac catctgccgt tactgatgca ctcgacgtaa tccagaatgt cacggcggtc
    16561 gagtaccggc accggctcac caaaggtgaa tgcctccatt ttcggggcgc tggcggtcat
    16621 tttttttgcc gcaggttgcg gtgttttccc ttttttcttg ctcatcagta aaactccaga
    16681 atggtggatg tcagcggggt gctgataccg gcggtgagtg gctcatttaa cagggcgtgc
    16741 atggtcgccc aggcgaggtc ggcgtggctg gcttcctcgc tacggctggc ctcataggtg
    16801 gcgctgcgtc cgctgctggt catggtcttg cggatagcca taaacgagct ggtgatgtcg
    16861 gtggcgctga cgtcatattc cagacagcca cggcggatga cgtcttttgc cttgagcacc
    16921 attgcggttt tcatttccgg cgtgtagcgg atatcgcgcg cggcgggata gaacgagcgc
    16981 acgagctgga acacgccgac accgaggccg gtggcatcaa ttccgatgta ttcgacgttg
    17041 tatttttcgg tgagtttgcg gatggattcc gcctgagtgg caaagtccat gcctttccac
    17101 tggtgacgct caagtattct gaatttacct ccggccacca ccggcggtgc cagcaccacg
    17161 catccggcgc tgtcgccacg gtgtgacggg tcgtaaccaa tccataccgg gcgggagccg
    17221 aacggattgg cggcaaacgg cgcatagtct tcccattctt ccagcgtgtc gaccatgcag
    17281 cgttgcagct cctcgaacgg gaacaccgac gccttgtcgt caacaaattc acacatgaac
    17341 aggtttttaa aatcgtcggc gctgttttca cgtttgagct gctcaatgtc gaacagcgtg
    17401 cagccacctt tcagggcgtc ctcaatggtg acaatctgcc gccactggcc gtccgcacag
    17461 agaagccccc cggcaagggc gttatgactg acgtcgattt ccacgcgttc ggcggcgctg
    17521 gcgcgtcccc ggttgaacag ttcacccgac cagaatgggt aggcgtcgtg cgccagcgtg
    17581 gacggggtgg agaaataggt cgagcgcagg tggctctgtg aggccatacc tgatgccacc
    17641 tttcgcagta cctgaaaatt cgggatccag aaaatctcgt cgacgtacag gtcgccgtta
    17701 tggctctgtg cggtgttgga gttggtgccg agaaaaatca gttttgcgcc gttattgccc
    17761 aggacaatcg ggtcaccggt caggtcaacg tcaaccagcc gggcaaaggc gatgatgtat
    17821 tcgcggaaca catacgcctg cgttttactg gccgacagaa aaatctggtt atgaccggtt
    17881 ttcagggcgc gcagcagcgc ctcgcgggaa aaataaaacg tcgcgccaat ctggcgggat
    17941 ttcaggatat cgcggatgcg gtgctcaagc ccggcgcgat accagtgcaa ctgatagtcg
    18001 aaagactgct caaagaaaat ctgctccagc ttttcgatgg cctcgtcact gaaaaaattc
    18061 tttttcggtt tgcgccgccc gcctttgttg cggttagcga cgttcggatt aaggtctgcc
    18121 tcgttgccgg tctggctgta gcggttgacc cgtgccagtc gttcaatctg gcgtcccagc
    18181 aggtcaattt ccttgaagtc actgccggtt ttctgcggtt tgatgatgag ctgggtcagc
    18241 cgcgcttcca ggctcatttc gacacggctg atgggggcaa cgctgtccca gccgtcgcgc
    18301 tgtttccagc tctgcaccgt cgggcgtttc atctgcaaca tggcggcaat ctgcggcacg
    18361 gaaaacccct gccagtacag cagcgccgcc tgacgacgcg ggtcgtgtaa aagagtggtg
    18421 tctgtggtga tggtcatgaa tacctcgccg tgatgaatac acggcaaggc tactgagtcg
    18481 cgccccgcga ttcgctaagg tgctgttgtg tcagtgataa gccatccggg actgatggcg
    18541 gaggatgcgc atcgtcggga aactgatgcc gacatgtgac tcctctaatc actattcagg
    18601 actcctgaca atggcaaaaa aagtctcaaa attctttcgt atcggcgttg agggtgacac
    18661 ctgtgacggg cgtgtcatca gtgcgcagga tattcaggaa atggccgaaa cctttgaccc
    18721 gcgagtctat ggttgccgca ttaacctgga acatctgcgc ggcatcctgc ctgacggtat
    18781 ttttaagcgt tatggcgatg tggccgaact gaaggccgaa aagattgacg atgattcggc
    18841 gctgaaaggc aaatgggcgc tgtttgcgaa aatcaccccg accgatgacc ttatcgcgat
    18901 gaacaaggcc gcgcagaagg tctacacctc aatggaaatt cagccgaact ttgccaatac
    18961 cggcaaatgt tatctggtgg ggctggccgt caccgatgac ccggcaagcc tcggcacgga
    19021 atacctggaa ttctgccgca cggcaaaaca caaccccctg aaccgcttca aattaagccc
    19081 tgaaaacctg atttcagtgg caacgcctgt tgagctggaa tttgaagacc tgcctgaaac
    19141 cgtgttcacc gccctgaccg aaaaggtgaa atccattttt ggccgcaaac aggccagcga
    19201 tgacgcccgt ctgaatgatg tgcatgaagc ggtgaccgct gttgctgaac atgtgcagga
    19261 aaaactgagc gccactgagc agcgcctcgc tgagatggaa accgcctttt ccgcacttaa
    19321 gcaggatgtg actgacaggg cggatgaaac cagccaggca ttcacccgtc tgaaaaacag
    19381 tctcgaccac accgaaagtc tgacccagca gcgccgcagc aaggccactg gcggtggcgg
    19441 tgatgccctg ctgacgaact gctgaccggc gtcagtcagt ccgggaaaac cttcacgatt
    19501 aacccttaat ttcaggaaaa actatgcgcc aggaaacccg ctttaaattt aatgcttacc
    19561 tgtcccgtgt tgccgaactg aacggcatcg acgccggtga tgtgtcgaaa aaattcaccg
    19621 ttgaaccgtc ggtcacccag accctgatga acaccatgca ggagtcctct gactttctga
    19681 cccgcatcaa cattgtgccg gtcagcgaaa tgaaagggga aaaaattggc atcggtgtca
    19741 ccggctccat cgccagcacc accgacaccg ccggtggcac cgagcgtcag ccgaaggact
    19801 tctcgaagct ggcgtctaac aagtacgaat gcgaccagat taacttcgat ttttatatcc
    19861 gctacaaaac gctggacctg tgggcgcgtt atcaggattt ccagctccgt atccgtaacg
    19921 ccattatcaa acgccagtcc cttgatttca tcatggccgg ttttaacggc gtgaagcgtg
    19981 ccgaaacctc tgaccgcagc agtaatccga tgctacagga tgtggcggtc ggctggctgc
    20041 agaaataccg caatgaagcc ccggcgcgcg tgatgagcaa ggtcactgac gaggaagggc
    20101 acaccacctc tgaggttatc cgcgtgggta agggcggtga ttatgccagc cttgacgcac
    20161 tggtgatgga tgcgaccaac aacctgattg agccgtggta tcaggaagac cctgaccttg
    20221 tggtgattgt gggacgtcag ctactggcgg acaagtattt tcccatcgtc aacaaggagc
    20281 aggacaacag cgaaatgctg gccgctgacg tcatcatcag ccagaaacgc atcggcaacc
    20341 tgccagcggt acgcgtcccg tacttcccgg cggatgcgat gctcatcacg aagctggaaa
    20401 acctgtccat ctactacatg gatgacagcc atcgccgcgt gattgaggaa aacccgaaac
    20461 tcgaccgcgt ggagaactac gagtcaatga acattgatta cgtggtggag gactacgccg
    20521 ccggttgtct ggtggaaaaa attaaggtcg gtgatttctc cacacaggcc agggcaaccg
    20581 cagagccggg agcgtaaccg atgacgagtc ccgcacagcg ccacatgatg cgggtctcgg
    20641 cagcgatgac cgcgcagcgg gaagccgccc cgctgcgaca tgcaactgtc tatgagcaga
    20701 tgctggttaa gctcgccgca gaccagcgca cactgaaagc gatttattca aaagagctga
    20761 aggccgcaaa aaaacgcgaa ctgctgccgt tctggttgcc gtgggtgaat ggcgtgctgg
    20821 agcagggcaa aggtgcacag gatgacattc tgatgacggt catgctgtgg cgtctggata
    20881 ccggcgatat tgccggtgcg ctggagattg cccgttatgc cctgaagtac ggtctgacca
    20941 tgccgggtaa acaccgccgc accccgccgt acatgttcac cgaggaggtg gcgctcgcgg
    21001 ccatgcgcgc tcacgctgcc ggtgagtctg tggatacccg cctgctgacg gagacccttg
    21061 aactgaccgc cacggctgac atgcctgatg aagtgcgcgc aaagctgcac aaaatcaccg
    21121 gtctgtttct gcgtgacggt ggtgatgccg ccggtgcgct ggctcacctg caacgtgcga
    21181 cacagctcga ctgtcaggca ggcgtcaaaa aagagattga acgactggag cgggagctga
    21241 aaccgaagcc ggagccgcag cccaaagcgg ccacccgcgc cccgcgtaag acccggagcg
    21301 tgacaccggc aaaacgtgga cgcccgaaaa agaaagccag ttaacaacga atgcgccccg
    21361 cgccagggcg gcacgccggt cagtgagggt gaatcacctg acgctgtacc ggcgtccacc
    21421 gcccgacttt tcagaggtag tcatgatgac gctgattatt ccgcgaaagg aggctcccgt
    21481 gtccggtgag ggtacggtgg tcatcccgca accggcaggc gacgagccgg tgattaaaaa
    21541 cacgttcttt tttcccgata tcgacccgaa gcgcgtccgg gaacgtatgc gccttgagca
    21601 gaccgtcgcc cccgcccgtc tgcgtgaggc catcaagtca ggcatggctg aaacaaatgc
    21661 ggagctgtac gagtaccgcg aacagaaaat tgccgccggt tttacgcgtc tggcggacgt
    21721 tccggcggac gacatcgacg gtgaaagcat caaagttttt tactacgagc gcgccgtgtg
    21781 tgcgatggcg accgcgtcgc tttatgagcg ttatcgcggc gtggatgcca gtgcgaaagg
    21841 cgacaagaag gctgacagca ttgacagcac cattgatgaa ctgtggcggg atatgcgctg
    21901 ggcggtggcg cgtatccagg acaagccgcg ctgcattgtg agtcaaatct gatgaagacc
    21961 tttgcgctac agggcgacac gctcgacgcc atttgtgttc ggtattacgg gcgcactgag
    22021 ggcgtggttg agaccgtgct cgccgcaaat ccgggactgg ctgaactggg tgcggtgctg
    22081 ccacacggca ccgccgtcga actgcccgac gttcagaccg cgcccgtggc tgaaactgtc
    22141 aatctgtggg agtaacgcat gacagcagaa gaaaaaagcg tcctgtcgct tttcatgatt
    22201 ggggtgctga ttgttgtcgg caaggtgctt gccggtggtg aacccatcac cccgcgtctg
    22261 tttatcgggc gcatgttgct cggtggtttt gtctcgatgg ttgccggtgt tgttctggtt
    22321 cagtttcctg acctgtcact gtctgcggtg tgcggcatcg gctccatgct gggtatcgcc
    22381 ggttatcagg tgattgagat tgccattcag cgccgcttta agggcagggg gaaacagtaa
    22441 tgccggtaat taacacgcat cagaatatcg ctgcctttct cgacatgctg gccgtgtccg
    22501 aagggacggc gaatcacccg ctgacgaaaa accggggcta tgacgtgata gtcaccggac
    22561 tggacgggaa gccggaaatc ttcaccgact acagtgacca cccgttcgca catggccgac
    22621 cggcgaaggt gtttaaccgt cgcggtgaaa aatccacggc ctccggtcgc tatcagcagc
    22681 tttacctgtt ctggccgcac taccgcaaac agcttgccct gccggatttc agtccgttgt
    22741 cgcaggacag actcgccatt cagttgatcc gcgaacgcgg tgcactggat gacatccggg
    22801 cgggacgcat tgagcgcgcc atttcacgct gtcgcaatat ctgggcgtcc ctgccgggtg
    22861 ccggttacgg tcagcgtgag cattcactgg aaaaactggt caccgtctgg cgtaccgctg
    22921 gcggcgtacc ggcttaaacg gagtaaacac catgaagaaa ttatcccttt cactgatgct
    22981 gcacgtgtcg ctggcgctga tgctggcact gtccctgatt tacccgcaga gcgtggccgt
    23041 caattttgtc gctgcctggg cgattctggc gacggttatc tgtgtggttg ccggtggtgt
    23101 cggcgtttat gccactgagt atgcgctgga acgctacggg cgggagctgc caccggagtc
    23161 gctggccgtg aagattgtca tgtcgctgtt tttgcagccg gtgccgtggt gcagacgggc
    23221 ggcggctctg gtggtgatgg tggcgacatt tatctcgctg gtcgctgccg ggtggatttt
    23281 taccgcgctg atttatctcg tggcgtcggt gttcttccgg ctgatacgta cggcctgccg
    23341 tcaacgtctt gaggggcggg aaccatgtca aagctgatga ttgtgctggt tgtgttgtta
    23401 tcgctggcgg tggcggggct gtttctggcg aagcatgaaa acgccagcct gcgcgcctcg
    23461 ctggacaggg caaacaatgt cgccagcggg cagcagacga ccatcaccat gctgaaaaac
    23521 cagcttcatg ttgcccttac cagggcagac aaaaacgagc tggcgcaggt ggcactgcgt
    23581 caggagctgg agaacgccgc gaagcgtgaa gcacagcgcg agaaaaccat cacgaggtta
    23641 ctgaatgaaa acgaagattt tcgccgctgg tacggcgctg acctgcctga tgctgtgcgc
    23701 cggttgcacc atcgcccggc ctgcactgac gccagtgatt gtccacaacg cctgcccgaa
    23761 agtgagcctt tgcccgatgc cgggcagtga cccggagacg aacggtgatt taagtgccga
    23821 tatccggcag cttgagaacg cgctggcacg ctgtgccagc caggtaaaaa tgattaaaca
    23881 ctgtcaggac gaaaacgatg ctcaaacccg acagcctgcg cagggcgctg actgatgccg
    23941 tcacggtgct gaaaaccagt cccgagatgc tgcggatatt cgtggataac gggagtattg
    24001 cctccacact ggcggcgtcg ttgtcatttg aaaagcgtta cacgctcaat gtgattgtga
    24061 ccgactttac cagtgatttt gacctgctca tcgtgccggt gctggcgtgg ctgcgggaaa
    24121 atcagcccga catcatgacc accgatgaag gccagaaaaa gggcttcacg ttttatgcag
    24181 acatcaacaa tgacagcagc tttgatatca gcatcagcct gatgctgacc gagcgcacgc
    24241 tggtcagtga ggtggacggc gcactgcatg tgaagaatat tccggaaccc ccgccgccgg
    24301 agccggtcac ccgcccgatg gagctgtata tcaatggcga actggtgagc aagtgggatg
    24361 aatgagttta agcgttttga agaccggctg accggactga ttgagtcgct gtcaccgtca
    24421 gggcgtcggc gactgagtgc cgaactggcg aaacgtctgc ggcagagtca gcagcgtcgg
    24481 gtgatggctc agaaagcccc ggacggcaca ccctacgcgc cacgtcagca gcagagcgcc
    24541 agaaaaaaga ccggtcgcgt taagcgaaaa atgtttgcga aacttatcac cagtcgtttt
    24601 ttgcatatcc tcgccagccc ggagcaggca tcaatggagt tttacggcgg gaagtcaccg
    24661 aaaatcgcca gtgtgcatca gttcggtctg tcggaagaaa cccggaaaga cggtaagaaa
    24721 attgattatc cggcgcgccc tctgctcggc tttaccggtg aggatgtgca gatgattgaa
    24781 gagattatcc tggctcacct cgaccgttag ttgtgccatt cttgacacct catcgtcaca
    24841 ttgccgccgg tatgacccgg cggcatcctt cccgttatga acactctcgc aaatattcag
    24901 gaactcgcgc gcgcactgcg caacatgatc cgcaccggca ttatcgtcga aaccgacctt
    24961 aacgccggtc gctgccgtgt gcagaccggc ggcatgtgca ccgactggct tcagtggctg
    25021 acccatcgcg caggacgttc gcgcacatgg tgggcacctt ccgtggggga acaggtgctg
    25081 attctggccg tgggcggtga actcgacacg gcgttcgttc tgccggggat ttattccggc
    25141 gataaccccg cgccgtctgc gtcggcggat gccctgcata tccgtttccc tgacggggcg
    25201 gtgattgaat atgaacccga aaccagtgca ctcacggtaa gcggaattaa aacggccagc
    25261 gtgacggctt ctgattctgt tactgccacg gtgccggtgg tcatggtgaa agcgtcaacc
    25321 cgcatcactc tggacacacc ggaggtggtc tgcaccaaca ggctgattac cggcacgctg
    25381 gaagtacaga agggcgggac gatgcgcggc aacattgaac acaccggcgg tgaactctca
    25441 tcaaacggta aggtactgca tacccataaa caccccggcg acagcggcgg cacaaccggg
    25501 agtcctctat gacagcacgt tatctcggaa tgaatcgcag tgatggcctg acggtcactg
    25561 accttgagca tatcagccag agtatcggcg atatcctgcg cacaccggtc ggttcacgag
    25621 ttatgcgtcg tgattacggc tcgttgctgg cgtcaatgat tgaccagccg cagaccccgg
    25681 cgcttgagtt gcagattaag gtcgcctgtt acatggcggt gctgaaatgg gaaccccgcg
    25741 tcaccctgtc atccgtcacc actgagcgca gttttgacgg gcgaatgacg gtcacgttaa
    25801 ccggccagca caacgacacc ggccagccac tttcgttaac catccctgtg agttgaaacc
    25861 atgccgatta tcgacctgaa ccagctaccc gcaccggatg tggtcgagga gctggacttt
    25921 gaaagcattc tcgctgaacg caaggcgaca ctgatttccc tttacccgga agatcagcag
    25981 gaggcggtcg cccgtaccct gacactggaa tctgagcctc tcgtcaaact gctggaagaa
    26041 aatgcttatc gtgagcttat ctggcgtcag cgtgtgaatg aggccgcacg ggcggtgatg
    26101 ctggcctgtg ccgccggtaa tgaccttgat gtgattggtg ccaattacaa caccacgcgc
    26161 ctgactatca ccccggcaga tgattcgacc attccgccga caccggcagt gatggaatct
    26221 gacaccgatt atcgtctgcg tattcagcag gcttttgagg gcttaagcgt cgccggggca
    26281 gtgggagcct atcagtatca tggtcgcagt gctgacgggc gtgtcgcgga tatttctgtc
    26341 accagtccgt ctcctgcctg tgtcaccatc tctgtgctgt cacgtgaaaa taacggcgtc
    26401 gcatccgaag acctgctggc tgtggtgcgt aacgccctta atggcgagga cgtcaggccg
    26461 gtggccgacc gcgtgaccgt gcagtctgcc gccatcgttg aataccagat aaacgccacg
    26521 ctttaccttt accctggtcc cgaaagcgaa cccatccgcg ctgccgctgt gaaaaagctg
    26581 gaagcgtata tcacggcaca gcaccggctg gggcgcgaca tccgtctgtc tgccatttat
    26641 gccgctttgc atgtggaagg tgtgcagcgt gtcgaactgg ctgcaccact ggccgacatc
    26701 gtgctcaaca gtacgcaggc gtctttctgt accgaatacc gcgtcgtgac cggaggctcg
    26761 gatgagtgat tcgcgactgc tgccgaccgg ctcatcaccg cttgaggtcg ccgccgcaaa
    26821 agcctgtgcg gaaattgaaa aaacgccggt cagtattcgt gagctgtgga acccggacac
    26881 ctgtccggca aatctgctgc cgtggctggc gtgggcgttt tcggtcgaca ggtgggatga
    26941 aaagtggccg gaagcgacaa aacgcgccgt tatccgcgat gcccatttca tccactgtca
    27001 taagggcact ataggtgcaa tccggcgtgt ggtggagccg ctcggctatc tcatcaacgt
    27061 gacggagtgg tgggaaaaca gtgacccgcc cggcaccttc cggcttgata ttggtgtact
    27121 ggaaagcggt atcacagagg caatgtatca ggaaatggaa cggctgattg ctgatgccaa
    27181 acctgcaagc cgccacctta ttggcctgaa cattacccgg gacattcccg gatacctgtt
    27241 cgccggtggt gtggcttacg acggcgatgt aattacggtt taccccggat aagtgaggaa
    27301 taatgagcac aaaattcaga accgttatca ccactgccgg tgcagcaaag ctggcagcgg
    27361 caaccgcacc gggagggcgg aaggtcaaca ttaccactat ggccgtcggg gatggcggtg
    27421 gtaaattgcc tgtcccggat gccggacaga ccgggcttat ccatgaagtc tggcgacatg
    27481 cgctgaacaa aatcagccag gacaaacgaa acagtaatta tattatcgca gagctggtta
    27541 ttccgccgga ggtgggcggt ttctggatgc gtgagcttgg cctgtacgat gatgcgggaa
    27601 cgttaattgc cgtggcgaac atggccgaaa gttataaacc cgcacttgcc gaaggctcag
    27661 ggcgttcgca gacctgccgc atggtcatca tcgtcagcag tgtggcctca gtggagctga
    27721 ccattgacac cacaacggtg atggcgacgc aggattacgt tgatgacaaa attgcagagc
    27781 acgaacagtc acgacgtcac ccggacgcct cgctgaccgc aaaaggtttt actcagttaa
    27841 gcagtgcgac caacagcacg tctgaaacac tggccgcaac gccgaaagcg gtaaaggccg
    27901 cgtatgacct tgctaacggg aaatataccg cacaggacgc caccatagcg cgaaaaggcc
    27961 ttgtccagct cagtagtgcc accaacagca cgtctgaaac gctcgccgca acgccgaaag
    28021 cggtaaaggc agcatatgac cttgctaacg ggaaatacac tgcacaggac gccaccacaa
    28081 cgcgaaaagg ccttgttcag ctcagtagcg ccaccaacag tgattctgaa acgcttgcgg
    28141 caacgctaaa ggcggtaaag gctgcgtatg accttgctaa cgggaaatac actgcacagg
    28201 atgctaccac cgcgcgaaaa ggtcttgtcc agctaagtag cgccaccaac agtgattctg
    28261 aaacgcaggc tgcaacacca aaggcggtga agtctgccta tgacaatgct gaaaaacgtc
    28321 ttcagaaaga tcagaatggt gcggatattc cgggaaagga taccttcacg aaaaatatcg
    28381 gtgcctgtcg tgcttatagc ggcgctttga gtactgaagc cggaaactgg acaaccgctc
    28441 agtttattga atggctggat tcccgtggtg catttaatca tccgtactgg atgtgcaaag
    28501 gctcctggtc atatgcaaat aacaaaatca ttacggatac cggatgtggt gatatccacc
    28561 tggctggttg tgtcgtcgag gtcatgggaa ttaaatctgc aatcactatc cgagtgacca
    28621 cgccgacaac atcaagtggt ggcggtacaa ccagcgcgca attcacttac attaatcatg
    28681 gggacggcta ctcccccggc tggcgtcgtg actggaatcg tcagggcgac gaaatgaccg
    28741 gaacgattaa tcaggacggc ggtagccaga atgcctatat gtctacggcc ttatgttcag
    28801 gcaccagagg cggcaaaaaa tatctcagaa agtttcgtgg tggagaagga gacactgtct
    28861 ggcatgaaac agtacagggc ggggtagttc gctgggcgac tggtaatact gatgctcagg
    28921 aagaattatc actcagctcc gcttatggtc tccgttcaag aggtgagatt acatcactca
    28981 gtgctaatgg tctgcgcatt gcttatggca attatggatt ctttatcagg aatgatggcg
    29041 gcagcacata tttaatgctg acggcctctg gcgataaatt tgggacatgg aacggtttaa
    29101 ggccgctgac tatcaataac gccaatggcg gagtgtcaat ggggcatggc ctgagtgtta
    29161 caggtgatat tgtctcaagg accaaagtac gtgccggtag cgggaaaaag ttcacggtca
    29221 gcagcagcaa tacatccacg aaggaagccg cattcaattt gtggggaaac tcaagtcgtc
    29281 cggtggtggc tgaattaggt gatgatgcag gctggcattt ttacagtcag agaaatacag
    29341 ataacagcat cacttttgct gttaacgggc aggtatcacc atctaactat agtaattttg
    29401 attcacgcta tgtccgggat atccggcttg gtggtgctgc cacatacaaa cctgcgaaca
    29461 atggcatgac atggacacat caggcaccgt ccgggtgtgt atattccggc attattgttc
    29521 aggataccgg ctcaaactct gccgataaca ttggtggcgt atattacaga ccggtgcaga
    29581 aatacattaa cgggacttgg tataacgtgg cgcaggtata atttatgcag catttgataa
    29641 atataacggc aggtaatcca aaaacggttg aacaatatca attgacaaag gactttgatg
    29701 ttgtctggtt tttttcagaa gatggtaaga actggtacga agaacaaaag tattttgctg
    29761 atgacacgat aaaaatagcg tacgacaaag ataatattat ccgctatgtg gaaaaggatg
    29821 tgacagctat cagaccggat ggattaagtg ttgttgaagt gccggatatt actgctaacc
    29881 gacgggcgga catttcaggg ggctggatgt ttaaggacgg tacagtgatt aaacgaattt
    29941 atacggcaga ggaattgcag cagcaggcag aaaatcggaa agccagactt cttgcagatg
    30001 ctgaatccgt gattttgccg ctggagcgcg ctgtcaggct gaacatggca acagaggagg
    30061 agcgcacacg gctggaggct tgggaacgct acagcgttgt ggtcagtcgt gtggatcctg
    30121 caaatcctga atggccggaa atgccgcaat aagttgtatg aactctggtg tgagcttaca
    30181 tatctatggc acagagtaaa gcctaatctg acagtccgct ctgtgccagg agcagacatt
    30241 gccaacgtta tgaccctcga ttacatcaaa gagccctata gccgaaacct gcaccatagc
    30301 catctctgta gaattcgtcc taggggcttt taacagacct agttgtatgg atcggaagga
    30361 aatattaaag ctcaaggcgt cagtccagtg ctgtcctgtt ggcttaccac acgaaagagt
    30421 gctgattgta cctgataact aatggatgcg gagtgagggg ggcgggtaga accaaaagca
    30481 cttactacgt gtggcgctct cctggaagag gagacgtcga agtgataacg ctaacaacag
    30541 tcttactggt gatgcaaata atgctagctg gtctggttgc catcaagcaa ctgaccggac
    30601 gcccgtaagg gatggaggaa cttgattaaa ggcagggggg ttacctcccc tgcctttttt
    30661 cgtttctgcg caagttagct ctgttattat aatctttact cacactgtaa ggacatagtt
    30721 caaatgactg aatggcttaa tgcacatttt ggtgcagacg cagctaccta tcttagctta
    30781 ttagtcggcg tactggcact gtttggtatt gggggcggtg ttacatatag aatcagacag
    30841 caaaacaaca taaaacagaa ggcaaagaaa accgagggtg atgtgaacca ggccgggcgg
    30901 gatattaatc agaccaccaa cgtttaccac acatccaatg aatctactga ggaccctgta
    30961 aaaaaaaagc aagatgaaca tgatctgaag ataatcgacc agatccttga attgctgcct
    31021 tatgaggata ctactcaatg ggttgagcag agctacctga gcggtatacg gcaagatgtt
    31081 tgcttaaaca ttgaaaaagc tgaaaaattt agcggtgaaa agtatcggtt attcaatact
    31141 gaagtgaatg gtgcgaaaga cgcttttttg gacgctatga ccgcatacgt agatagtacg
    31201 ctaccattta tgagtgttga ttacccgaac agaaagccag tgatgctaag ccttccccat
    31261 gactggaaga gtaagagtgt tgaatcggag gcaaacttcc gctttcatca gcaaaatgtg
    31321 cgtgaaacat cgggggggat gattgcgcgc tatgaggatt ttgtcaggac atttaaggct
    31381 catggattca taagcgataa actgtaaagg cgcagtgggt cagcgaacac cgcaacgaca
    31441 gaaccgcgta agcggtaaaa tcagcttgtt aactggcgag tttatagccg tccctccggg
    31501 gcggcttttt tatgactata atcgacagaa tcacctgaac gtccaggtgg gtttccctga
    31561 caggacgtca aggaatgttc gctttgtgcc aggagcggac gttgccagca ttgagttttc
    31621 agttttcaaa ggtatatctt tagatttagt tcacttagaa tgagcgtgtt aaagttttcg
    31681 gttatgtgag tatattgaac ataacaaata aacaataatt tttgtattcc atttaggata
    31741 taaaaaagag ggatttgagt tatgtacgat tatagttttt tacttactcc atctacagga
    31801 tttgttaaaa gattatgtgg agtttggtcg aatgatttct ctataaatga tgattctgac
    31861 tctgatttag ggcaaacaga tattcattta ctttcatctc ctcatttgaa tatcatatcc
    31921 gacccaaatg aattggcttc caagttaaaa ggtttgttga tgctcatgaa cggcgcactt
    31981 agcgttcttt atggttttga aagatatcaa tcttttggtc ctctttctat ttctggaagt
    32041 gaaggcatta attactcgga aatatgcggc tttaaatcaa tggatgtaac tcaggtaaac
    32101 ccctttaatt tccatgacat acagcaacca caccctcgcg ctgataattt ttcacgatta
    32161 attaatttat catctaaaca tgaatcctta cgtgttgttt taggtatgtg tgccttaggt
    32221 gatgattggg ttaatcttta tcgtttgtgg gaaacaatta gagaatatgt atatgataaa
    32281 tatgtgaaag gtatactaaa ttttcccgac acttataaag gcaagaaata tcagagagat
    32341 aaagtaatta gcatggcttt taatattgat gaaaaggaaa tcagcagatt cacgggcact
    32401 gctaatagct ttgaactgtt agggtatctt gcgagacatg gaaagggaag ttccggaggg
    32461 atattaaaaa atccgatgtc aagagttgac gcaagcattt tcgtacacaa agcaacttac
    32521 aaatattgta agttattttt gatgtaagta aaaagttgaa gctataatac tcaaagtatc
    32581 gaaaactacg ttcgtcgtgc actcgaaaac aatatgctta ttgtgtctgc actaattact
    32641 caaggtccga gaaaaatggc gaagctcatc tttttcaggc ttcgccaact tgagttattg
    32701 gttaaaattc atttaggaat aatagaatgc aattcgtgac tcacctggat ttcatagtgt
    32761 ttcaatagtt ctccgatgtt aattcctact accttacgaa ctgcgccgag ggagaagtta
    32821 agctcctcaa tgcccggccg atctggcgtt gaattcatat agtgtcggca cgcacggaca
    32881 attgactcga cgatcaacga tagcggagac tcaggtgcaa tctcggagcg aatcgccgta
    32941 agagagagtc ggagctcctt caaagaatcc gagactcgtg ctggatcctc ttgagcatat
    33001 ggcagataaa gagaactctt gtcttccaat aaatggatca cccgcagagc aattttctcg
    33061 tctgggcgat gcgatagaaa ttgaaaaatt agcctctttc ctccctccaa gcactccttc
    33121 attagtgatt cgtggtcacg tttccatttt cgcaaatact cggcggggta gcccataccg
    33181 ccattcttat caactaaatc agcacaagct ccgcacaacc atatagcatt ttttatgctc
    33241 ttccgctctg tttctgactg agacatgtca tagcgcggtc cgccagggct cgccgcgcag
    33301 atatgcgccg cctcacccgt cttgatagaa agattttccg ccaattgatc cgggccgatt
    33361 gttaccctat gacaagaggg attgctacaa atatatgcag cgcgctctgc taccttacct
    33421 acaacgcttg gaggaaagtt gtgcctattt gtcttggtca taaaagttat gcttctttgt
    33481 ttttttcatc tatgtgggga gaataaaatc atacttttta tatctgatcc tagggctgac
    33541 cagcaaccta ttgatcaaaa aaccaagata ttagctaaaa aaaatcagca tacttgagac
    33601 caagctcaat gtccgctcct cgctcaaagc agactgtcag atttgatagc gtttgggcta
    33661 tgtaaattgt cagttggaaa atgagtgagt gcaaatcatg acaggcaggc ggattacccg
    33721 ctttttcttt gtctgttgtt tcatccactg accagccagg tcaaatagcg tctcatgcac
    33781 tgcccaacag aaaatagttg cacccattaa ccacggagtt aaacggatga gtgactatca
    33841 tcacggcgtg caggtgctgg agattaacga cggcacccgc gtcatttcca ccgtatccac
    33901 tgccattgtc ggcttggtct gcacggccag cgatgcggat gcggaaacct tccccctcaa
    33961 taaacctgtg ctgattacca atgtgcagag tgcaattgca aaggctggta aaaaaggcac
    34021 gctggcggca tcgttgcagg ccatcgctga ccagtcaaaa ccggtcaccg ttgttgtgcg
    34081 tgtggaagac ggcaccggcg acgacgagga aacgaaactc gcgcagaccg tatccaatat
    34141 catcggcacc accgacgaaa acggtcagta caccggactg aaagccctgc tggcggcgga
    34201 gtcggtaacc ggtgttaaac cgcgtattct cggtgtgccg ggactggaca ccaaagaggt
    34261 ggctgttgca ctggcatcag tctgtcagaa gctgcgcgct ttcgggtata tcagcgcatg
    34321 gggctgtaag accatttccg aggtgaaagc ctaccgccag aatttcagcc agcgtgagct
    34381 gatggtcatc tggccggatt tcctcgcatg ggatacggtc accagcacca ccgccaccgc
    34441 gtatgccacc gcccgtgcgc tgggtctgcg tgccagaatc gaccaggagc agggctggca
    34501 taaaacgctg tccaatgtcg gggtgaacgg tgttaccggc atcagcgcat ctgtattctg
    34561 ggatttgcag gagtccggca ccgatgctga cctgcttaac gagtcaggcg tcactacgct
    34621 gattcgccgt gacggtttcc gattctgggg taaccgtacc tgctctgatg acccgctttt
    34681 cctctttgaa aactacaccc gcaccgcgca ggtgctggcc gatacgatgg ctgaggcgca
    34741 catgtgggcg gtggacaagc ccatcaccgc aacgctgatt cgcgacatcg ttgacggcat
    34801 caatgccaaa ttccgtgagc tgaaaacaaa cggctatatc gtggatgcga cctgctggtt
    34861 cagcgaagaa tccaacgatg cggaaaccct caaggccgga aaactgtata tcgactacga
    34921 ctataccccg gtgcctcctc tcgaaaacct gaccctgcgc cagcgtatca ccgataaata
    34981 cctggcaaat ctggtcactt cggttaacag caattaagga gactgaccga tggcaatgcc
    35041 gcgcaaactc aagttaatga acgtctttct gaacggctac agctatcagg gcgttgcaaa
    35101 gtccgtcacg ctgccaaaac tgacccgtaa gctcgaaaac tatcgcggtg cggggatgaa
    35161 cggcagcgca ccggtagacc tcggccttga tgacgatgcg ctgtcaatgg agtggtcgct
    35221 cgggggcttc ccggattcgg ttatctggga gctttacgcc gcaaccggtg tggatgccgt
    35281 gccgattcgt tttgcaggct cttaccagcg cgacgatacc ggcgaaacgg tggccgtcga
    35341 agtggtcatg cgtggacgtc agaaagaaat cgacaccggc gagggtaaac agggagaaga
    35401 caccgagtcg aaaatctccg tggtctgcac ctatttccgg ctgacgatgg acggtaagga
    35461 gctggtcgaa atcgacacca tcaacatgat tgagaaggtg aacggcgtcg accggctgga
    35521 gcaacaccgc cgaaatatcg gcctgtgatt ttcatccggt cagcctggct gaccggttaa
    35581 ccctgattca gaagtgagaa aaccatgaac aaagaaaatg tgattaccct ggacaatccg
    35641 gtcaagcgtg gtgagcaggt aatcgaacag gtcacgctga tgaaacccaa tgccgggacg
    35701 ctgcgcggtg tcagtctggc tgcggtcgcg aactccgaag tcgatgcact gattaaagtg
    35761 ctgccgcgca tgacggcacc gatgctgacc gaacaggagg tcgccgcgct ggaactgcct
    35821 gaccttgtgg cgctggccgg taaggtggtc ggttttttgt cgccgaactc ggtgcagtga
    35881 cgtttccgaa aaatctctcg gtcgatgacc tgatggcgga tgtggcagtg atatttcact
    35941 ggccgccatc agaactgtat cccatgagcc tgaccgaact catcacatgg cgcgaaaagg
    36001 cgctccggcg aagcggaaac acgaatgagt aacaatgtaa aattacaggt attgctcagg
    36061 gctgttgacc aggcatcccg cccgtttaaa tccatccgca cagcgagcaa atcgctgtcg
    36121 ggggatatcc gggaaacaca aaaatcactg cgcgagctga acggtcaggc atcccgtatt
    36181 gagggatttc gcaagaccag tgcacagctc gccgtgactg gtcatgcact tgaaaaggct
    36241 cggcaggaag ccgaagccct tgccacacag tttaaaaaca ccgaacgtcc gacccgtgct
    36301 caggcgaaag tgctggaatc cgcaaagcgt gcggcggagg acttacaggc gaaatataac
    36361 cgcctgacgg attctgttaa acgccagcag cgggaactgg ccgttgtggg aattaatacc
    36421 cgcaatcttg cacatgatga gcagggactg aaaaaccgta tcagtgaaac caccgcacag
    36481 cttaaccgtc agcgtgacgc gctggcgcgt gtcagtgcac aacaggtaaa acttaacgca
    36541 gtcaaacagc gttatcaggc aggaaaggaa ctggccggaa atatggcctc agtgggcgct
    36601 gccggtgtgg ggattgcagc tgcgggaacg atggccggag ttaagctgct gatgcccggt
    36661 tatgagtttg cgcagaaaaa ctcagaatta caggctgtgc tcggtgtggc aaaagactcc
    36721 gccgaaatga ccgcactccg caagcaggcg cgccagctcg gcgacaatac cgcagcctcg
    36781 gcagatgatg cagccggtgc acagattatc attgcgaaag cgggtggaga tgctgcggct
    36841 attcaggcgg caacgccggt cacgctgaat atggcactgg cgaatcagcg gtcgatggaa
    36901 gaaaacgcgc aactgttgct ggggactaag gcatcctttc aactgtcaaa tgatgatgtc
    36961 agccatgtgg gcgacgtgtt gtcggcaacg atgaataagt cggcggctga ttttcaggga
    37021 ctcagtgatg cactgactta cctcgggccg gttgcgagga cggcaggtgt aagtcttgag
    37081 caggcagcag ccatgacagg tgtgctgcat gacaataaca tcagggggtc aatggcgggg
    37141 acgggtagca gtgccgttgt cacccgatta caggcaccga ctggaaaagc atgggatgca
    37201 ctcaaagagc ttggcgtgaa aacctcggac aaaaagggaa atatgcgtcc gttattcacc
    37261 attctgaaag agattcaggc cagctttgat aaacacaagc tgggaacgtc tcagaagggg
    37321 gaatacctta aaaccatttt tggtgaggaa gccctgaaat cagcgaacgt tttactggca
    37381 gcggcagcaa gcggaaaact ggataagctg accgccacgc tgaaagcctc ggacggtaaa
    37441 acggaagagc tggttaaaat catgcaggat aacctcggcg gtgactttaa ggagtttcag
    37501 tccgcttatg aggcggtggg gactgacctg tttgaccagc aggaaggcgc gctgcgtaag
    37561 ctcacgcaga cagccacaaa gtatgtgtta aaactcgacg gctggatcca gaaaaacaaa
    37621 tcactggcgt caaccatcgg catcattgcc ggtggcgcgc tggcgcttac tggcatcatc
    37681 ggtgccattg gccttgtagc ctggccggtt atcaccggca tcaatgccat catcgcggca
    37741 gcaggcgcaa tgggggcaat cttcacgacg gttggcagtg ccgttatgac ggccatcggg
    37801 gcgattagct ggccggttgt ggctgtggtg gccgccattg tcgccggggc gttgcttatc
    37861 cgtaaatact gggagcctgt cagcgcattc tttggcggtg tggtggaagg gctgaaagcg
    37921 gcatttgcgc cggtggggga actgttcacg ccacttaagc cggtgtttga ctggctggga
    37981 gaaaagttac aggccgcgtg gcagtggttt aaaaacctga ttgccccggt taaagccaca
    38041 caggacaccc tgaaccgttg ccgtgacacg ggcgtcatgt tcgggcaggc actggctgac
    38101 gcgttgatgc tgccgcttaa tgcgttcaac aaactgcgca gtggtattga ctgggtactg
    38161 gaaaaactcg gtgttatcaa caaagagtca gacacacttg accagaccgc cgcaaggact
    38221 aatgccgcca cgtatggcac cggtggttat attccggcga ccagctctta tgcaggctat
    38281 caggcttatc agccggtcac ggcaccggct ggccgctctt atgtggacca gagtaaaaac
    38341 gaatatcaca tcagcctgac gggcggtact gcgccggaga cacagcttga ccgccagtta
    38401 caggatgcgc tcgaaaaata cgagcgggat aaacgtgcgc gcgcccgtgc cagcatgatg
    38461 catgacggtt aaggaggtga cgaaaaatga tgctcgcgtt aggtatgttt gtttttatgc
    38521 gccagacgct gccacaccag accatgcagc gtgaatcaga ttatcgctgg ccgtcaaatt
    38581 cccgtatcgg taaacgggat gcctaccagt ttctcggtgt gggtgaggaa aacatcacgc
    38641 ttgccggtgt gctttatcct gaactgaccg gcggaaagct gacgatgacc acgctcaggc
    38701 tgatggcaga ggaaggccgg gcgtggccgt tgctggatgg cacaggcatg atttacggca
    38761 tgtatgtcat cagcagggtg agtgaaacag ggagtatttt ctttgcagac ggcacacccc
    38821 ggaaaattga ttttacgctg tcactcaccc gcgttgatga atcactggcc gcgctttatg
    38881 gcgatatcgg taaacaggcg gagtcgctca tcggtaaggc tggcagtctg gcgaccagat
    38941 tcacggggat gacgggggcg gaataatgct ggatgcgctg acatttgatg caggcagtac
    39001 gctgacgccg gattacatgc tgatgctcga caacagggat attaccggca atatcagcga
    39061 ccgtctgatg agcatgaccc tgacggataa ccggggcttt gaggctgacc agcttgatat
    39121 tgaactgaac gatgccgacg ggcaggtcgg gctgccggtt cgtggcgctg tcctgacggt
    39181 gtatatcggc tggaaaggtt ttgccctggt atgcaaaggg aaatttaccg ttgatgaggt
    39241 tgaacaccgg ggcgcaccgg atgtggtcac catccgcgcc cggagtgcag attttcgcgg
    39301 gacgctcaat tcccgccggg aaggctcatg gcatgacacc acgctcggtg cgattgttga
    39361 ggcgatagcc tcccgtaaca ggctggaagc cagtgtcgct ccgtcactgg ccggaattaa
    39421 aatcccgcac atcgaccagt cgcaggagtc tgatgcgaaa tttctgaccc gtcttgcaga
    39481 acgcaacggc ggtgaggtgt cggtaaaaat gggaaaactg ctgtttctca aagcggggca
    39541 gggggtgacg gccagcggta aaaaaatccc gcagattacc atcacccgca gcgacggcga
    39601 ccgtcatcat tttgcgattg ctgaccgtgg agcttacacc ggcgtaacgg caaagtggtt
    39661 acacaccaaa gacccgaagc cgcaaaagca gaaggtaaaa ctgaaacgca aaaagaaaga
    39721 aaaacacctg cgcgcactgg agcacccgaa agcgaaaccg gtcacgcaga agaaagcgcc
    39781 aaaagtaccg gaagcgcgcg aaggtgaata catggccggt gaggctgaca acgtttttgc
    39841 cctgaccacg gtatatgcca cgaaagcgca ggccatgcgc gccgctcagg cgaagtggga
    39901 taagctgcaa cggggcgttg cggagttctc catcagtctg gctaccggtc gtgctgatat
    39961 ttacacggaa acaccggtta aagtgtcagg ctttaagcgc gtcatagacg agcaggactg
    40021 gacaatcact aaggtgacac attttctgaa taatagcggc ttcacgacgt ccttggagct
    40081 tgaggtcagg ctttctgatg tggagtacga aacagaagat gatgagtgat gtttttattt
    40141 tatctgtttg ttttataagg ataaattaac taaaatggca ccatcaacaa aaccggaaga
    40201 ggtgctcgcg atgtttcatt gtcctttatg ccagcatgcc gcacatgcgc gtacaagtcg
    40261 ctatatcact gacacgacaa aagagcgtta tcaccagtgc cagaacgtga attgcagcgc
    40321 cacgttcatc acttatgagt cggtacagcg atacatcgtg aagccgggag aagtccacgc
    40381 cgtaaggccg cacccgttgc cgtcagggca gcaaattatg tggatgtaat tacaaacagg
    40441 aagcccctca gtcgaggggc ttttttgtcg atgtggtcaa tgtgtggacg tgaccagaaa
    40501 taaatccttt tatttcattg tattacgcgt aaaaaataag cccgtgtaag ggagattaca
    40561 caggctaagg aggtggttcc tggtacagct agcattttat gggttatgtt tttcagcgaa
    40621 acggatgata accttaataa atgcagctgt atgtgatcgg tttctaagaa ttttccatcc
    40681 gggaaaaata atcgaaatta atcacttacc gtggggatta cgcgtggttt ccccggagaa
    40741 attacgcatc agcagcgcgt aattgagctc aagatcctgc gggaccggga gccacacagt
    40801 ataaccatcg cctggtgcta tcggcatagc ttcgcctttg gcgttttcca tgtgctcaag
    40861 ggtaaaatta atgttgcctt gcggcgtcat cagctcaagg ctgtcgccaa cggagaattt
    40921 atttttcacc gctaccgccg cgaggtcccc cttgcgctca ccggtaaact caccaacaaa
    40981 ctgctggcgg tcagaaactg aataaccgta ttcgtagttc tgataatcgt cgtgagtatg
    41041 acgacgcagg aaaccttcgg tatagccacg atgcgccaga ccttccagag tttccagcag
    41101 gctggtatcg aacggttttc ccgcagcggc gtcatcgata gctttgcggt aaacctgtgc
    41161 ggtgcgtgca caatagtaga aagatttggt acgaccttcg attttcagcg aatgcacgcc
    41221 cattttggtc aggcgttcta catgggcgat ggcgcgcaga tctttcgagt tcatgatgta
    41281 agtgccgtgc tcatcttcaa acgcggtcat atactcgccc ggacgctggg cctcttcgat
    41341 cataaacact ttgtcggttg gtgcgccgat acccagcgtc ggctcaacat tttgcaccgg
    41401 aatcggctcg tacttgtgta cgatgttgcc aacatcatct tctttccctt cctggacgtt
    41461 gtactcccag cggcaggcgt tggtgcaggt gccctggttc gggtcgcgct tgttgatata
    41521 gccagagagc aggcagcgac cggagtaggc catgcacagc gcgccgtgaa cgaagatctc
    41581 gatctccata tccggcacct gattgcggat ctcttcaatc tcttccagcg acagctcgcg
    41641 agagaggatc acgcgggtca ggcccatttg ctgccagaat ttcaccgtcg cccagttcac
    41701 ggcgttagcc tgcaccgaaa ggtggatcgg catttcaggg aagtgctcac gcaccagcat
    41761 aatcagccct ggatcggaca taatcagcgc atccggcccc atttccacca ccggtttcag
    41821 gtcacggata aaggttttca gcttggcgtt gtgcggtgca atgttgacca cgacataaaa
    41881 ctttttcccc agcgcgtggg cttcattgat gccgagctga agattttcgt ggttgaattc
    41941 gttgttgcgc acacgcaggg aataacgcgg ctggcccgca taaacagcat ctgcgccata
    42001 agcgaaagcg taacgcatat ttttcagcgt tcccgccggg gaaaggagtt ccggtttaaa
    42061 cataattctc tcgttctgat gacaggtcag atccgcttca cctgatgaag cggttagggg
    42121 agtgccccca ctttaagggc gggcattgta gcgcaatcgg cgggtttagc tccagtgtaa
    42181 atgtctggtg gcgtcctggt tttggttaag gatacgaatg acgataattt cccctggtga
    42241 ttgcaggaaa taaatgacgt ggcgttcaaa aggaagaaca aaaatacctt cgcctaattc
    42301 aggtctgggc gtacctatat tattgttact cagaatctgg agaacgtctg aaagatgttc
    42361 cacatatctg tcggcttgtg gttcgccgaa atggtgatag ctgtaatacc agatgccttc
    42421 taaatcctca ttggcttttg gcatgagttt aataatacgc acattatttc cttatccctg
    42481 ctttcacctt ccgtaagaac gcgtcctttt cccacggctg agcttcacca ctactcaacc
    42541 cttctgcgag catgtcacgt aacgcttgca ggcgagactc tgcttgcttt tctcgaagta
    42601 atcgcagtga ctcacggatc acttcgcttt gggtgcgata gtcgccggat tcaattaagg
    42661 actcgataaa ttcacgcaac tcatcgccga gatcaaccgt catcgttctg gccattttac
    42721 gcccgcctgt aagtaaaaat cttacatcag tgtatgtcat aaaccggata gcgtcacgaa
    42781 agtattagaa gatattttgg cacatgaaca tgttcaggag gataagtatg ggatggtaaa
    42841 caaagaaagc acaacgagtg ctcgtcagtg cccactattt taatggcggc ggttactctt
    42901 aattattaat aactaagcgt tatttggata taaagccgga cgccaacaac ccacgcccgg
    42961 catcgtaatt acgtattgtc tttcaccgtc tggcttgtgc cattggcttt aacggaggca
    43021 attccggttt cacgagattg cgcggtggcg tacatctggc tggagccgat aatttgatga
    43081 ttagcggctt tcagattgaa atagaatttg ccgttacttg ccgttttttt ctcatagcgt
    43141 tcttcttgcg ggctgttgct acgcaccgac gcgatgccct tttccgcaga ggcttttgag
    43201 gtatacagct cgctggtgag gatagtctca ccattgcccg cttttagcac aaaccggaac
    43261 tgattatcac tgctcttact gagttcaaac caaccagcca taatgactcc ttgattgaaa
    43321 ttgtgtatcg aaaacagcat ctaaaggtgc tgtttaccga ttaaagtatg agcgattttc
    43381 tgatgcctgc cagggcggag agataatgtc agtttgctga ccatgcgtgg gataatgttg
    43441 ccggatctgg tctgaacgcc atctccggct aacaaagtca tgtcgctaaa actaaacgat
    43501 gcggcaggcg tcggcttccc agcggtaacc aacgccgtaa acggcgcgga taaatgactg
    43561 ttcggcgtcg agagattcca gcttgcggcg caggttttta atgtggctgt cgatggtgcg
    43621 gtcggttact acgcggtagt cgtcataaag atgattgagc aaatgctcgc gggagaacac
    43681 ttttcctggt tcatgagaga gcgttttcag cagacgaaat tccgcaggcg tcaggtcaag
    43741 cattttaccg cgccatgaag cctgaaaacg accttcgtcg ataatcaacg ggctttcagc
    43801 atcctgttgc tgcaactcgc gctgcggttt gcaacggcgc aaaatggttt tgacgcgcgc
    43861 taccacttcc cgtgggctgt acggcttaca gatataatca tctgcgccaa tctccagccc
    43921 cagcaggcga tcgatctctt cgatttttgc cgtcaccatc acgatcggaa tgtcagaaaa
    43981 acgacgaatt tcccggcaca gcgtcaggcc atcggtgcca gggagcatca gatctaacag
    44041 gatcagatcc ggtggtgtct ggcgtacata cggcagtacc tgatcgccgt ggctgataag
    44101 cgtcggcgca tagctcgcag cacgcagata atcaatgagc aactgcccca gcttcggttc
    44161 atcttccacg atcaaaatac gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct
    44221 ctctgtaaat cccgttccag cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa
    44281 tgggcagcaa taatgcgacc attatgtgct tcaacaatgt tcaggcaaat cgccagcccc
    44341 agcccggaac cgccgctggc acggttgcgg gaaccttcgg tgcgataaaa acgttcaaac
    44401 aatttttgta gctgatcgtc actgacacct ggcgcactgt cggcaaaggt caggcgcacc
    44461 gttttgtcgc gctgaccggc agagatttgc aggctgccgc cgctgtcagt gtagcgcagg
    44521 ctgttttcca gtaagttatt gaataactgc attaaacggt cgcgatcgcc aaatacggta
    44581 atactgtctg gcagggaaaa ttgcagtttc aggccacgac tggcgaatcg ttcgcgaaat
    44641 gcgccgcccg ccacttccag cagtgggatc aaatctaccg gtgctttttg ataggcgaga
    44701 gcgccttcat cagacatcga caactgatgg agatcgtcaa ccagtttggt cagtgtaccg
    44761 acctccgcct gtaaagacgc caccgtctcc ggcgtgaatt tacgcacgcc atcctgaatg
    44821 gcttccagtt caccgcgcag caccgctaat ggcgtacgca gttcgtgaga aatatcggcc
    44881 ataaaatcgc gccgcatttg ctggtttttc tccagcgtgc tggcaagctg gttgaagtct
    44941 tgcgccagct tgcccagttc atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc
    45001 gccgccagtt tgtgcgtgcc atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc
    45061 agcagaaaag tggcgagtgc cgcgagtaac gttgccagag cgacaatcaa ccagctggtt
    45121 tgccgctgtt gtttatcgaa attgatatca gtattgcgcg ttaaccgctc aacgggggag
    45181 gcgatcaccg cgccaacttc cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt
    45241 ggaatcggcg ctcgcggacc aaccagcact ttgttgtttt gatcaaccac ccagaactgg
    45301 gtacgccagc cgtgcggtgg catacccggg ccgggtttat cttccgaatt atcgtgttca
    45361 aatgaacgca ggatctgaaa gacaaagcga tcattgttgc gcaggaagcg ccagttgcca
    45421 tgctgcgcat actgctcgcc aagcgcatca cttaacagtt gtaatcgctg ttcattacca
    45481 tgcttgatgt aatcaataaa gccacgctca aaactgatac gcaccgccca gtgcatactg
    45541 atcagcaaga caatgcaggt ggcgaaaatc gccagaaaca gtttgccggt aataccgggt
    45601 cgccagaact tcattgcgcg ctcctttttc gccgcgaaat agctacattt tgatgcgtat
    45661 cgttcggcac tctggcaaag atgaacgccg gaagggcgat gatcaacgcc atgctaagcc
    45721 aggtgtacat aaagacggtt tgtgtggtgc cgctgtcgac gctgacatgc tgtgaaccaa
    45781 aaagtcccag caacagcccg gcgatagtga cgccgatact catcgacaat tgcataatca
    45841 tcgacagcag gctgttgccg ctgctcgcca gattgtccgg gagatctttc agcgtcaggg
    45901 tgttcatgga ggagaaacgc gtcgagttga ccatcccttg taaaaacagg acgaacggca
    45961 aaacgtagta ccagcccagc agggcggtag tcataaacaa cagggtgacc agcgacagac
    46021 ccagcgtggt cgctaccagt acccgacgat aaccaaagcg attcaccaca tgtaccacaa
    46081 ttcgcttcat tcccatgctg ccaagcacca tcgggatcat catcagtccg gcatgaaacg
    46141 gcgagaaacc gaggccaatt tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc
    46201 caatacgtcc ggcaaagctc cccgccaggc ccagcgaaaa ggtacgagta cggaacagtt
    46261 tcagactgaa cagggcacgg ttgttatttc tggcgtgcag cagataaagc accagtgcca
    46321 ccacgccaac tgcgaccagg cctgcaatcg tcagcggcga taaacctgta cctttactgc
    46381 cgtccagcgc cagggttaat accgccatgc caaccgccag caataaaaat ccggagagat
    46441 caaagcgccg cgtctgcatg gtgtagtttg gcattaacag caatgtggcg atcgcaccga
    46501 taatccccac cggaatgttg atcaaaaaga tccagtgcca cgatgcgtac tccaccagca
    46561 gaccgccgag cgccggaccg agcagcggac cgacctgacc gggtaacgtg acaaaggtca
    46621 tcgccgccat atattgctcg cgcggtacga ttttcatcac cgtcaatctg ccgaccggca
    46681 ccatcatcgc gccgccaacg ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc
    46741 cggaaagcgc gcaaaacagt gaaccgagag taaacagcac gatggcggta aagaaaatat
    46801 tgcgcacgcc gactttgtcc gccagccagc cgctggcggg cagcatcacc gccacggtca
    46861 gcacataaga gacaatgacc atgtgcatat gcaacggact ttccccgagg ctttgcgcca
    46921 ttgagggaag ggcggtgttt acgatggtgg tgtccagcga ctgcataaag aagccgaaag
    46981 ccacaatcca caattgccaa cgggtgctgt cgggaagatc tgtcatttac tcggttaccg
    47041 tttgtttagg tttacgcgaa aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg
    47101 gcgtggtata cagcgtaagg agctggctca ttaccagtcc gccgacaatg gtgatcccca
    47161 ggggttgccg cagctccgag ccgtcgccgc ccgacaatac cagcggcagc gcaccaaaca
    47221 gcgccgccag ggtagtcatc ataatcgggc gaaaacgcag cagacaggcc tggaaaatag
    47281 cttcctgcgg cgtcaggtta ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa
    47341 tggcgttttt cttcacgatg ccgattaata gcatgatccc tatcagggcg attaggctga
    47401 acggggcatt gaacagctcc agcgccagca gcgctccaac gcccgccgag ggcagggtgg
    47461 agaggatcgt cagcggatgt acgtaactct cgtaaaggat acccagcacg atatacaccg
    47521 tggcgatggc ggcgataatc aggatcacct gcgagttcat cgtctcctgg aacacctgcg
    47581 ccgtgccggc aaaactgccg cgcaccgtcg aaggcacccc aagctgggtc attgcgcgat
    47641 cgatcgccgc actggcgtcc gagagcgatt ttccggtcgg caggttaaac gaaatggtcg
    47701 aggccgccga taatccctga tgattcaccg atagtggggc attcgccggt tgccatttag
    47761 cgaaatacga cagcgggatc gctttgcctt cgttattgat aacgaacatt ttttccagcg
    47821 cactgatgtc ctgggtatag cgcggatcca cttccatcac cactttatac tggttcatcg
    47881 gctggtaaat ggtcgagatt tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg
    47941 cttgtacgtc gattcccagc cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg
    48001 cgccgttatc ctgctgatcg gagttcacgt ccgccagttc cggcaacgtc gccagttttt
    48061 tgcggatttt cggctcccat tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact
    48121 gatagctggc gttcgactga cgtccgccaa cgcgaatatc ctgtaccgcc atcaggaaca
    48181 gattcgcccc cggttctttc gccagcttca cgcgcagacg gtcgataatt tgctgcgccg
    48241 tttcgctgcg ttcgtcgcgt ggcttgaggg tgataaacat catcccgctg ttcactcgcg
    48301 aaccgccggt aaatccggtg acattatcca ctgccggatc gtcacggata attttcatga
    48361 aatcctgcaa cttaccgcgc atcgcctgaa acgaaatact ctgatccgcc tgtatcccgc
    48421 ccatcaacac gccagtgtcc tgctccggga agaaggtttt cgggatcgag atatatagcc
    48481 agatattcag cgcaatggtg ccaagcagca ccacgcccac cagacgggta tgattgagca
    48541 cccatttcag cgacttgccg tagccttgtt gcagggctac caacatgcga ccaaaaccac
    48601 gcagtcgctt ttgctcacgc ggcttgctgg ctttcagcat ccagccacac atcatcggcg
    48661 ttagcgtcag ggaaaccagc aacgaaatac caatggcgac agaaagcgtc acggcaaact
    48721 cgcgtaacag tcggcccggc aatccgccca tcaacagcag cggcaggaac accgccacca
    48781 gtgacagact catcgacagc accgtaaaac cgacttcgcg agtaccttgc agtgcggcct
    48841 gtagtggttt catccccgct tccagatgac gggcaatgtt ttccagcacc acgatggcgt
    48901 catccaccac gaaaccagta gcgatggtga gcgccattaa cgacaggtta ttgagactga
    48961 atccgcacag gtacatcgcc gcaaacgtac caatcagcga aaccggcacc acaacggcgg
    49021 gaataatagt ggcgcgaccc gagcgcagga ataaaaacac caccagaatc accagcgcca
    49081 ccgagataat cagcgtttgc tcgacttctt ccagcgaggc gcgaatggtg ggggagcgat
    49141 cctgggcaat ttgcagatca atcgccgccg gaatggtctc ctgcaactcc ggtaattttg
    49201 cccggatgct gtcaaccgtc tggataatat tggcttccgg cagcttgcgg atcatcagca
    49261 aaatagctgg tttggcgttg gtcatcccgg cgttgcgcac atcctgcacc gagtcagtca
    49321 ccgtcgccac atcacccaga cgaaccgcgc cgccgttgtt gtaatgaata atcaacggct
    49381 gatattcagc ggcggttttt agctcatcat tggtctggat ctgccagcgg tgagtgccat
    49441 cttccagtgc cccctgtggt ttacgcacgt tggcattgct gatggcggtg cgtacgtcgt
    49501 ccagcgagac gccctgatta aacagcgcct gcggattcag cccgacgcgt acggcgggca
    49561 gtgagctgcc gccgacatcg acatcaccaa caccgtcgat ttgcgaaatc gtcggagcca
    49621 gctgcgtcga ggcgaaatcg tacagttcac cctgagaata agtatcggat gtcagcgtga
    49681 ggatcataat tggcgcatcc gacgggttcg ctttgcgata ggtcggacgg ctgggcatcc
    49741 cactgggcag caaactttgt gcagcgttga tcgccgcctg cacatcacgc gctgcgccgt
    49801 tgatatcccg gtcaaaatca aactgcaaaa taatccgcgt gctgccgagc gaactactgg
    49861 aggtcatttc actaaccccg gcaatgcgcc caagtgagcg ctccagcggc gtggcaacgg
    49921 aagacgccat tgtctccggc gacgcaccgg gcagcgaggc gctgaccata atcaccggaa
    49981 aatcgacctg cggcagcggg gcgaccggca gcatacggaa gcccagtatg ccgcagaggg
    50041 taatggcgac cgacagtaaa atcgtcgcca ccgggcggta aatgaagagg gcaaaaaact
    50101 tcacttacgc ctcctcttca tggcgagcaa agcgactttt ggtccacaat gccaggcggt
    50161 cgaacagcag ataaatcacc ggcgtggtga acagcgtcag cacctggctg acaatcagac
    50221 cgccgaccat gccgatacct aacggacgac gcagttccgc gccgaccccg gtactcaaca
    50281 tcagcggcag cgcgccaagc agagccgcca gggtggtcat caggatcgga cgaaaacgca
    50341 acagacaagc ctggtagatg gcatcgcgcg gcgacatgcc ttgctcgcgc tcagcagcca
    50401 gcgcgaagtc gatcatcatg atggcgttct tcttcacgat accgatcagc aaaataatgc
    50461 cgataatcgc aatcacatcc agctcacttc cggcaatcat taatgccagc agtgcgccga
    50521 cccctgcggt gggtagcgtc gagagaatgg tgatcgggtg aataaagctc tcgtacagaa
    50581 tgccgagcac gatatacatc gccaccaccg ccgcgacaat cagccagaca gtgctgccca
    50641 gcgccgactg gaaggcgagg gtgctgccct ggaattgcgt ggtgatatcc accggcagat
    50701 tcagcgtctt ctcggtgtcc ataatcgcct gcaccgcatc acccagcgaa tagttatccg
    50761 gcacgttaaa ggagatggtc gttaccggga actgatccag atggttgatg gagagcggcg
    50821 caaaacgctg ctcaattttg gcaattgagc ttagcggcac cacgccaccg tcgctgctgg
    50881 tcaggcgaat ggtatccagc gccgcgaggc ctggggtatt ttcggtgttg tgttccagca
    50941 ccacgcgata ctggttagcc tgagtataaa tagtggaaat cagccgctga ccaaacgcgt
    51001 tgtacagggc gttatcgaca tccgccatgc tgatccccag acggctggcg ctgtcgcgat
    51061 caacattgac atacgccacc agccctttgt cctgccagtc gctggagaca tcagaaagct
    51121 gtggcagttg ctggagtttt tccatcaact ctggcaccca ggtactgagc gcatccagtg
    51181 aggtggcctg caaggtaaac tggtactggg tgcggctgac ctgagtatcg atggtcaggt
    51241 cctgcgttgg ttgcaggaag agatcgacgc ccggcacttt atccaccgcc gtttgcagac
    51301 gggcgatgac tttttgcacc cgatcatcac gttcatccag cggtttgagg ttgatctgca
    51361 aacgcgcact gttcagcgac gggttagtgc catcaacgcc aacaaatgag gtcagacttt
    51421 gcactgccgg atcctgcaaa atcacgtccg cgacctggcg ttgccgctgg gccatattgg
    51481 caaaggagct ggattgcggt gcctgcaaag tgccctgaat aatgccgttg tcctgtaccg
    51541 ggaagaaacc tttcggaatg aacacccaca gcagcacgct aagcagcagc gtgctgagtg
    51601 ccacgcttaa ggtcagccac ggatgattca gcactttcgc cagtccacga ccataggcgg
    51661 cgattatcct gtcgaacatt ttttccgagg cacgggagaa gcggttctgt ttacgcaacg
    51721 actcctggct gagcatccgc gcgcacatca tcggtgtcag ggtcagcgac accaccgctg
    51781 agatcaaaat cgctaccgcc agggtaatag caaattcgcg gaacagtcgc ccgacgatat
    51841 cgcccataaa cagcagtggg atcaacaccg caatcagcga gaaggtcagc gagataatgg
    51901 taaagccgat ttcacctgcg cccttgagcg ccgccgccaa cggtttttcg cctttttcga
    51961 tatagcggga aatattttcg atcaccacga tggcgtcatc gaccacgaac ccggtggcga
    52021 tagttaacgc catcagcgtc aggttattga ttgaaaaatc gagaaacacc atgaccgcga
    52081 aggtgccgat taacgacagc ggcactgcga agccgggaat gatggtcgcc ggaatattgc
    52141 gcaaaaacag gtagataatc atgactacca gcgcgatagc catcatcaat tcaaactggg
    52201 tatcgttgac cgatgcgcgg atattggtgg tgcgatcgga aagcactgtt accttcaccg
    52261 atttcggcag actctcggtg agctgtggca gcatctgccg gatgctgtcg gcggtggaga
    52321 taatgttagc accgggctgg cgctgaacat tcatcacaat ggcctgttct ttgttcgccc
    52381 atgcgccaag ccagctgttt tctgcacctt gctctacggt tgcgacatcg ccaagacgaa
    52441 tcggcgcgcc gttctggtag gcgatgatta gctggcgata ctcttcggcg gattgcatct
    52501 ggtcgttagc ggaaagcgta accgcacggg aagggccgtc gaggctacct tttgccgagt
    52561 taacgttagc gccggtaatg gcggtgcgca cggtttcgct ggtcaggccg agggcggcaa
    52621 tcgcctgagc gttaagtttg acgcgaacag ccggacgctg accgccggaa agcgtcacca
    52681 gtccgacacc ggaaatctgc gagattttct gcgcgacgcg ggtttccacc atatcttcca
    52741 cttgcgtcat cggcatggcg gttgaggtga cggcgagcgt catgatcggc ggatctgccg
    52801 ggttcacttt gctgtaaacc ggcgggttag gcagatcgct cggcaacaag ttggtcgcag
    52861 cgttaatcgc ggcctgcact tcctgctcgg cgacatcgag cggtaatgtt agctggaact
    52921 gcaaagtgat aactgacgca ccgccggaac tttgcgacga catctgtttc aggccagaca
    52981 tctgcccgaa ctggcgttct agcggcgcgg taacggcaga ggtcatgaca tccgggctgg
    53041 cacctgggta gagcgtgacc acctgaatgg tcggatagtc cacttccggc agcgccgaaa
    53101 cgggcagggc gcgataaccg ataatcccgg cgagtaagat cgccaccatc agcagcgtgg
    53161 tggcgacagg acgcataata aaaaggcgcg acgggccgcc tgtgctgctc gggggtaaca
    53221 cctgcatcag gagcgtgctc cttttttcgc gtattcgcgg ctggtggctt tctcttccgg
    53281 agtggtggcg ctctgggctt ccaccacttc cactttcgcc ccttcggtca ggcgatcaat
    53341 gccgtctgtc accacgcgat cgcccgcaga aatacctgca cggatcacca ctttctgact
    53401 gtcctgaatg cccggcgtca ccagatgttt gctgaccttg ttttcgctat tcagcaccca
    53461 gacaaaatgg ccttcattgc ccatttgcag ggcggctgtt gggatcacta cggcgttttg
    53521 ttcggtgtcg actaacatgc gcgcgttaac aaactgattg ggaaacagcg catcatcctg
    53581 attattaaag cgtgctttca ctttaatcgt accggttgtg gcatcgattt ggttatcgag
    53641 gcttaacagc gtgccttcac ttaatttctt cgaattggtg cgatcccagg cttctaccac
    53701 cagcggtttt ccggcttttt gcgcctgcac tacagtagcg atatcgcttt ccggcagggt
    53761 aaagactaaa tcgataggat gcgtctgggt gatcaccaca attccggtgg tatcaccact
    53821 ggagatttgg ttaccaacat caacctgctt gagaccaacg cgaccatcga ctggtgcggt
    53881 gatgcggctc cagtcgagtt gcagctgcgc gctggcgacg cttgcttcat cagccttaat
    53941 ggtgccttcg gtttcactga ccagcgcctg ttgggcatcc agctcctgac gggagacgag
    54001 attggttttt gccagttgct gataacgcgc cagatcacgg cgggcgttag caagcgtggc
    54061 tttatctttt gccagttggc cctgggcttg tgctaatgca actttgaact ggctggggtc
    54121 aatttctgcc agtaaatcgc ctgctttgac ctgctggcct tcctggaaat gtaacgccat
    54181 cagttgaccg tccacgcggc tgcgcaccgt aacagtatta gcggcggtaa tggtgccaag
    54241 cccggtgagg taacgcggaa ctgcctgttc tacggcggtc gccgcctgaa ccggggctaa
    54301 tgggccggaa cgcataccac ggcgaccacc cgctggcgat tgctgcgctt gtttcgtcgc
    54361 ccctggggct gcactctggg agtcattgcg gccttgccag aaccagaatg cggcgatggc
    54421 ggcgataacc accacgatta cgattaccca acgggattta taactgcctt tcatcgttaa
    54481 gagtttctct tcctgaaacg tttcgcggaa tgatactagt gtagccagcc aataagggag
    54541 aaaaatggag gaattatgaa acactatcgg gaatcgtctg aatggcgggc acatttgcga
    54601 gcacgcatcc agtaataaca caggaaacta ttttatctac gcgttagcga tagactacat
    54661 acagggcgaa aggaggtaag ccgcagattt cagccggatg ctgaaacggg aaagcccctc
    54721 ccgaggaagg ggccttaaat aaggaaaggg tcatgatgaa gctactcatc atcgtggtgc
    54781 tcttagtgat tagctaccca gcatactaag actaccaggg cgggggaaac cccgctctac
    54841 cctcactcct gaaagtatgc cttcacgata agattgtcaa tccgcaggct ttgccgtctg
    54901 cgatcctgcc agcaaaaatt ctttgcgaga cgttacgcaa taatcacact ggaaactatt
    54961 ttatctgcaa gtcagcgata gactaagtgc atggcgaaag gagataagcc gacgatttca
    55021 gcgggacgct gaaacgggaa agcccctccc gaggaagggg ccttaaataa ggaaagggtc
    55081 atgatgaagc tactcatcat cgtggtgctc ttagtcataa gcttccccgc ttactaagac
    55141 taccagggcg ggggaaaccc cgctctaccc tcactcctga aagtatgcct tcacgataag
    55201 attgtcaatc cgcaggcttt gtagtctgcg atcctgccag caaatattct ttgcgagtcg
    55261 ttacgcaata atcacagagg aaactatttt attcacgcgc tagcgataga ctgcattcag
    55321 ggcgaaagga ggtaagccga tgatttcagc gggacgctga aacgggaaag cctctcccgg
    55381 agaagagggc ttttaataag gaaagggtta tgatgaagca cgtcatcata ctggtgatac
    55441 tcttagtgat tagcttccag gcttactaag aacaccaggg ggagggggaa acctctccct
    55501 aaccctcact cctgaaatta tgtcactccg cccttagtgt caatcgacgg ctctccgttc
    55561 ttgcgaggcg aatagcaaaa ttttctgcgc cgccgggcct aattctgaaa gtttcttcgt
    55621 ttgctcttat ttcattattg cagtcggtat gataccggac ctcgataatt ctctaagaaa
    55681 ggatttctta tgagcgaaca aatcacgttt gccaccagcg attttgccag taacccggaa
    55741 ccacgttgcc cctgcatttt gctgctggat gtttctggct cgatgagcgg cagacctatt
    55801 aatgaactta acgccggatt ggtgaccttt cgtgatgaac tgcttgccga tccgctggct
    55861 ttaaaaagag tggaactcgg cattgtgacg ttcggcccgg tgcatgtgga acaacccttt
    55921 accagcgccg ctaatttttt tccgcccatc ctgtttgccc agggcgatac accaatgggt
    55981 gcggccatta ccaaagccct ggatatggta gaggagcgga aacgtgagta tcgtgcaaat
    56041 ggtatttcgt attatcgccc gtggattttc ctgattaccg atggtgcacc aaccgatgag
    56101 tggcaggctg cagccaacaa agtgtttcgg ggggaagaag ataaaaggtt tgctttcttt
    56161 tccatcggtg ttcagggggc ggatatgaag acgctggccc aaatcagcgt tcgtcagcct
    56221 ttgcctctgc aaggattaca attccgtgaa ctgtttagct ggttatccag ttcgctgcgt
    56281 tcggtttccc gctccacgcc gggaacggaa gttgtgctgg aagcgccaaa aggctggacg
    56341 tcagtgtgag ctggcgtctg gtttatgcat ccgctgttgg aacatcgcac atcagcgcgg
    56401 acttgccttg ccaggatgcc tgccagatgc aggttgcctg gctaaatgac cagcagccat
    56461 tactggtgat gttccttgct gatggcgcag gtagcgtttc gcagggagga gaaggcgcga
    56521 cgctcgccgt caatgaagcg atggcttata tgtcgcaaaa agtgcagggt ggggaattgg
    56581 ggcttaatga tatcctcgcc acggatattg tgctgaccgt ttgtcagcga ctttttgccg
    56641 aggcggaagc caaagaatta gcagttcgcg attttgcctg cacgtttctg ggattgattt
    56701 catcgcctga cggcaccttg attatgcaga ttggcgatgg cggcgtggtg gtggattttg
    56761 gtcacggtct gcaattgccg cttacgccga tggtgggtga atatgccaat atgacgcact
    56821 tcattaccga tgaagatgcc gtttccagac tggaaacttt caccagcact gagcgtgtgc
    56881 ataaagttgc agcatttacg gatggtatcc agcgactggc gttaaatatg ctggataatt
    56941 ctcctcatgt acctttcttt accccgtttt tcaatggact ggcgtcagca acgcaggaac
    57001 aactcgattt attacctgaa ttgttaaagc agtttttgtc cagcccggcg gtaaacgagc
    57061 gtacggatga tgataaaacg ttggcactgg cgctgtggtt accatgaaac ccactttata
    57121 tactgctact ggtgagtgcg ttacgccagg ccgtgaactg ggcaaaggcg gtgaaggcgc
    57181 ggtttatgat atcaatgagt ttgtcgatag cgtcgccaag atttatcaca cgccgccacc
    57241 cgccttaaaa caggacaaac ttgcctttat ggctgcgaca gctgacgcgc aattgttgaa
    57301 ttatgtcgcc tggccgcagg caacgcttca cggtggacga ggcggaaaag ttatcggttt
    57361 tatgatgcca aaagtttctg gtaaagaacc gattcatatg atctatagcc cggcacatcg
    57421 tcgtcaacgt taccctcatt gtgcgtggga ttttctactc tatgttgcgc gcaatattgc
    57481 ttcatctttt gctacggttc acgagcacgg gcacgtcgtg ggtgacgtaa accagaacag
    57541 ttttatggta ggtcgcgaca gcaaagtggt attgattgat agcgactcct ttcagattaa
    57601 cgctaatggc acgttgcatt tatgcgaagt cggtgtgtcg cattttacgc cgccagagct
    57661 acaaaccttg tcgtcatttg tcggctttga acgcaccgcg aatcacgata attttggcct
    57721 tgccttgctg atatttcacg tcttgtttgg tggtcggcat ccttattctg gtgtgccgct
    57781 tatctctgat gcgggtaatg cgctggagac ggatattacc catttccgtt atgcctacgc
    57841 gtcagataat cagcgacgtg gtttaaaacc gccgccacga tcgattccgc tatcgatgtt
    57901 accgagtgat gttgaagcca tgtttcagca ggcattcacg gaaagtggcg tggcaaccgg
    57961 gcgtccgacg gctaaagcgt gggtagcggc acttgattct ctgcgccaac agttaaagaa
    58021 atgtcccgtt tcggcaatgc atgtttatcc cgctcatttg accgactgcc cgtggtgtgc
    58081 gctggataat caaggcgtta tctattttat tgatctcggc gaagaggtca ttaccaccgg
    58141 cggggatttt gtgctggcaa aagtctgggc gatggtgatg gcgtcagtag caccgccagc
    58201 attgcaactg ccattacccg atcatttcca accgactggc aggccgcttc ctttaggcct
    58261 gttacggcgc gaatacatca ttctgcttga gatcgcactg tcagcattat cgctgctgct
    58321 ttgcggcctt caggcagaac cgcgttatat tattttggtt cctgtgctgg cggctatctg
    58381 gattattggc agtctgacaa gcaaagcgta caaagcagaa gttcagcaac gccgtgaggc
    58441 atttaatcgt gcgaaaatgg actatgacca tttagtccgc cagatccagc aggtaggcgg
    58501 cctggaaggt tttatcgcca aacggacgat gctcgaaaaa atgaaggacg aaattctcgg
    58561 gttaccggaa gaagagaaac gcgctctggc agcacttcat gacactgcaa gggaacggca
    58621 gaagcagaag tttctggagg gattttttat tgatgttgcc tctattcccg gcgttggccc
    58681 tgcgcgtaaa gcggcgttac ggtcttttgg tattgaaaca gccgcagatg ttacccgtcg
    58741 tggcgttaag caagtgaaag ggtttggcga ccatctgacc caggcggtta tcgactggaa
    58801 agcgagctgt gaacgccgtt ttgtgttcag gccgaatgaa gcgataacgc cagcagacag
    58861 acaagcggta atggcgaaaa tgaccgccaa acgacatcgg ctggaatcga cgttgactgt
    58921 cggcgcgaca gagttgcagc gattccgcct tcatgctcca gcacggacca tgccgttgat
    58981 ggaaccgtta cgtcaggcgg cagaaaaact ggctcaggcg caggcagatt taagccgctg
    59041 ctgagccatt ttcttttaac gaaacaccac ttccgcccag cgtgccagcc cggcggtgac
    59101 ggagccaaag tcatcgccgc ctgcaatcgg aattcccggc aactgttctg ccagcgcttt
    59161 tttaatcagc ggcgaacggg cgctaccgcc ggtcaggtag ataacgtccg gtttttcctg
    59221 ggcgttatcc agggccagtt gcacctgttc cagaatccgc gccagtggct ggctgagggc
    59281 gctttccagc ccttgctggc taatcagcgt agccagttca tcgctgataa acggcagtga
    59341 ggcgcgggtt tccgctacgc ttgaaagagc aattttgcac tcttctgcgc tacgcaccag
    59401 gcgatagctt aaacgctgac gccagacttt ctgtagcagg gccacttttt ccggttcgcg
    59461 ggcatcgcgt accagatcgt taagcagacg accgttggca ctactgtaga aatcactctg
    59521 cgcaggtacg tcgttgatgg caaccgcatt ccaccacggc agaatcggca gggcgatgcc
    59581 tttttcggtt tcgccaccca tgcccagtaa cggcatcagg tttttaaacg ccagtgcgat
    59641 gtccagatcg ttaccgccaa tacggcaacc actgtgaccc agcaggctgg cttcacgatc
    59701 gagacgcgaa cgccactgcg gccccatcag cagcaatgaa cagtcagtcg taccaccgcc
    59761 gatatccacc accagcaccc gtttttcttc ctgcaaggtg gcttcgtaat ccagcccagc
    59821 cgcgaccggc tcgtactgga ataccacgtc cctgaatccg gcacgcttcg ccgcgcgttc
    59881 cagaatccct tgcgcctggg cgtttgcttc atcgccgccc agcccctgga agttgatcgg
    59941 gcgaccaatc accgcctgag taatcgcttc tggcagctgc gcctgcgcct gctggcgaat
    60001 gtgcaacatc attgcgcaga ccagatcctc aaacagcgct acctgctgcg gttttaagcc
    60061 gctggcaccg aggaacgatt ttggtgattt cacaaaccac acttcttctg gatcatcaat
    60121 gtactgtgcc agtgaggaaa gaccgaactg cacgcttttc gccgtaacat cgatatcttc
    60181 ttcgcggtta taacgaatcg cccgacgtaa cagcgcctgc gtttcatcgt cgtctgccgg
    60241 aacatcatga tggcggtaca gccattcact tactgcttca cgcgttggcg cgcaaagcat
    60301 tgaaggcagc agcgtgctgt cgttttccat tttcagtaat tgcggtttac cgtcacgcat
    60361 gaccgccact gaacagtttg ctgtaccgta atcaaaacca ataaacacga attaatcccc
    60421 atgccggtga agaaggggcg tgactttagc gaaatgttgc cgtcgcgaca accggaatat
    60481 gaaagcaaag cgtagcgtct gaataacgtt tatgctgaaa gcggatgaat aaggagatgc
    60541 gatgtatacc ctgaactggc agccgccgta tgactggtcg tggatgttgg gatttctcgc
    60601 cgcccgtgcg gtgagtggtg tggaaacggt cgctgaggat tattatgccc gtagtctggc
    60661 ggtgggcgaa tatcgcggcg tggtgactgc tattccggat atagcccgcc atactctgca
    60721 cataaattta agtgcaggtt tagaacccgt tgccgctgag tgtctggcga aaatgagccg
    60781 cctgtttgat ctgcaatgta acccgcagat tgttaacggt gcgttgggca agttaggcgc
    60841 ggcgcggccc ggattgcgtt tacccggctg tattgatgct tttgagcagg gtgtgcgggc
    60901 gattttaggc caactggtga gcgtggcgat ggcggcaaaa ttgactgcca aagttgtaca
    60961 gctttatggc gaacggctgg atgattttcc ggagtatatc tgcttcccca cgcctcagcg
    61021 gctggcagca gccgacccgc aggcattaaa agcgttaggc atgccgttga aacgggcaga
    61081 ggcactgatt catctggcaa atgcggcgct ggagggcagc ttaccaatga caattccggg
    61141 cgatgtggag caggcgatga aaacgctgca aacttttccg ggtatcgggc gctggacggc
    61201 gaattatttc gctttgcgtg gctggcaggc gaaagatgtt tttctgccgg atgattatct
    61261 gattaaacag cgatttccgg ggatgacacc ggcacaaatc cgccgttatg ccgagcgctg
    61321 gaagccctgg cgttcttatg cgctgttgca tatctggtat acggaaggct ggcaaccaga
    61381 cggaacggac gaattgtagg cctgatacgt cgcatcaggc aatgggcaaa cgctccctca
    61441 gttaatcgcg aaataactgc tattcaccag caaatccagc ggttgggcat cggcaatcac
    61501 atcaccataa atcagatcga cgccgatgcc agaaagcgta tccatcacta agggtaaaac
    61561 gactggcccg gcgatggttt tcatcccgag tcgctgagcg tgcccctgaa taatcgtaat
    61621 cagcatctca tccatcaaat taccctgcac gttggcgcat aactcaccat caagtagcag
    61681 gtaatctgcc atattcgctt tcagcgagtt gaagatttgc agatcgcggc ccacctgact
    61741 gagcactatc cgacatcccg ccagccgcag tttttgcacg ctttctgcgt gatctaaaat
    61801 cgcttctgcc ggaataatca gatgtaataa ccgtggtggt agagggctgt tttccagctg
    61861 ctcaagcagt tcattcacca gtgtggcgct actcaaaccg gcaacggaaa gggggagggc
    61921 gatgcttatg cctttactgg caaccgcttt tgcggcctgc tggaaaaatt cgtggaatac
    61981 ccgtcggtca agagcatggc taagtgccgg atcgctgaag ctacgacgaa atttttgttc
    62041 atcaataatc tcgccttcgc aactccagag cttaagtgaa atcagccaca aattacgcgc
    62101 ttccgggatc cgcggcgaag cgacaccgtg ggcgatcatc atcaactgat tctctttaat
    62161 catccgccac tgttcatcaa gcgacatcgc cgcccgttcg ctatgtgcgg cagcttgctg
    62221 tggttcgtaa accgtcaccc ggccccgtcc accatttttg gaggcataac aggcgatatc
    62281 agcctgcgac atcacttctg ccgcctgatg attgttgtca tcaatcaagg taatcccggc
    62341 actggcacct acccgatgca cacggccttc ccatataaag tgatagtcat tcacggcact
    62401 gataatgcgt gtagcgataa aacgcgcgct ttcaacatta cagtctggta gcagcagacc
    62461 aaattcatcc ccaccgagtc gcgccagcac gtcgctggaa cgcagcatac tcagcattaa
    62521 cgacgccagt tcgcgcagca aagcgtcgcc cgccgcatgc ccggcgctgt cattcaccgc
    62581 tttaaagcga tcaagatcga taaacaccag tgcatgtcgc tgatgcgtac tgtttaccgt
    62641 ttgcagcagg atacgcagtt gtttctcaaa actggcgcgg ttggcgagat gcgtcagtgc
    62701 atcatgggag gcgctgtagc tcagctggcg cagcattttg cgtgattcgg tgacgtcctg
    62761 aatcaccaga accgaaccaa tattgctgcc gtccagagta cttaacggcg taatactgta
    62821 atgcacgtcg tagctgccgc cgctccgaca gtgcaacacc acatcttgtt caatcgcgga
    62881 acgtgaggtg tcggcactgt aaatgttctc catcagggga ccattgtcgc caaaggtaat
    62941 atgcaacacc gtcaggagcg gaacacctaa tgcttcttcc tgcgtccagc cgctcatctt
    63001 ctccgccact ggattcataa aggtaatttt cattgccata tcaatacaga ccacggcttc
    63061 accgatggaa tcaagcgtaa tgtgcaggcg ctctttttcc tgaaacaatg cctcgttaag
    63121 ctgtttaacc tcggtcatat ccatattaat gccgagcagg cgttcgactt cgccttcttt
    63181 attcagtacc cggttggcga gggcgcggat atggcgaata ccgtctttta cggtaatgcg
    63241 aaattccagc ttaaagggcg agcgtgattg caacgaatca cgaatcactt tttcggcatg
    63301 ctggcgatct tccggcagca cgcactcgta ccacacctgc cagttcggtt tgatatgcgg
    63361 aggaatttca tacagctcga acatccgctt atcccagcta aaaatattcg gcttcagctc
    63421 ccactcccag atgccaatcc cgcccgcttc gttagccagc gtgatgcgct ccatcagttg
    63481 ctgattcacc tgttcggtgc gttttagctc gttaatgtct tcaatctgag cgataaaata
    63541 gagcggcgtg ccatccgtgt ggcgcaccag tgagacggca agcaacgccc agacaacatc
    63601 gccattgcgg ttgtagtagc gtttttccat tgaataggtg tttatttcac cgcttatcag
    63661 cttttcaacc tgttggagat ctttattgag atcctccggc caggtcagtt gctgaaaggt
    63721 gagtccgcgc agctcttcct gactgtaccc gagaaactgg cagagcgctt tgttggtttg
    63781 cagccattgt ccctcggtgc ccactaacgc cataccgata gcggaatatt ccatggcgtt
    63841 ccgaaaacgg gtttcgcttt cggaaatgtg tttgcgttcc gcacgaaagg catacatcac
    63901 catggtcatg atgttggcgg gcagcaggat cagcaaaaac ggtagccacg gcatatggct
    63961 catcaggtat gtacgcggcg tagcaagcag ggagggatcc gcggccatca tcagcgacac
    64021 catcatcacc gtggtaagga agatcaaaaa agcttccatt cgtggcaggc gcacggcgct
    64081 ccacatcaac agcacaataa tgaaagtaaa aggccacggc agatacagca tcgaaagcca
    64141 gcttaacgtc agtgtgatgg ctaacgtgag cagcgactca aaaagcaacc gtgggttgcg
    64201 atggcgcagc agatagtgtg gtttaaataa caatcccagc ggcaccagtg ccagagcgcc
    64261 gatggattct gacagtaccc atatcaaaaa tgccctgaga ggatcgtctc cgggcgtcag
    64321 caggacaacc agaacacccc ctagcagagg cggaacaatg gcgctgccga atgccagacg
    64381 cagccagtca gccagatttt gcaaggggtt ataccacggc agcaatttac gtagcagcac
    64441 tgccccgacc acggcttcaa caatattgat ggtcgtccag gtcatgttca gcgagctggt
    64501 ggaaaaaagc aggatggatg cggcgatatt tcccagcgaa cagctcagcg caattcccgg
    64561 ccacatgcgc ccggcatggc gataaaacgc caccatcatg atggacgtcg ggaaccacag
    64621 tggggcgagt tgggtgccaa actgcgaaag ctcaagcgag aaaagggtaa agataaacga
    64681 gactaaacct aaactgacca ggtgaagcag cgggtggggc agggcaatta atacatgctg
    64741 tgattgtttg ctcattaccg ctttatccgc aagacctggt cgccagtatt cgtttctgtg
    64801 tttatgctag tacaaaagtt ttacatttta tatgcgaatt gctcataaag tgacgtaaag
    64861 gcggataatt tgcgcaactg cgtataacat tttttacctt acataaaact gatcaacgta
    64921 atttgccccc gggaattctt tgtcaacaat taactctgag cgcgcggcaa cgctattcga
    64981 ctggtatcag acggatgaaa tccctataat tgccgcgttt ggcgcttcgt cgcccccttc
    65041 ctaacatcca ggttaatcag gtcgctaaat atatgactga tcagtctcat cagtgcgtca
    65101 ttatcggtat cgctggcgca tcggcttccg gcaagagtct tattgccagt accctttatc
    65161 gtgaattgcg tgagcaagtc ggtgatgaac acatcggcgt aattcccgaa gactgctatt
    65221 acaaagatca aagccatctg tcgatggaag aacgcgttaa gaccaactac gaccatccca
    65281 gcgcgatgga tcacagtctg ctgcttgagc atttacaagc gttgaaacgc ggctcggcaa
    65341 ttgacctgcc ggtttacagc tatgttgaac atacgcgtat gaaagaaacg gtgacggttg
    65401 agccgaagaa ggtcatcatt ctcgaaggca ttttgttgct gacggatgcg cgtttgcgtg
    65461 acgaacttaa cttctccatt ttcgttgata ccccgctgga tatctgcctg atgcgccgca
    65521 tcaagcgtga cgttaacgag cgtgggcgtt caatggattc agtgatggcg caatatcaaa
    65581 aaaccgtgcg cccgatgttc ctgcaattca ttgagccttc taaacaatat gcggacatta
    65641 tcgtgccgcg cggcgggaaa aaccgcatcg cgatcgatat attgaaagcg aaaataagtc
    65701 agttctttga ataagcttga taaattgtgt accgtttagt gataacctgg tatgcccttg
    65761 acgtaattgg cgttaaggga gtgatgcgcg aaaggagaaa atgccatgcg tctgtgtgac
    65821 cgagatattg aagcctggct tgatgaaggc cgtttgtcga tcaacccacg tccgccagtg
    65881 gagcgtatta acggcgcgac ggtggatgta cgcctgggca ataaatttcg taccttccgt
    65941 ggtcacacgg cagcgtttat cgatctgagc ggtcccaaag atgaagtgag cgccgcgctt
    66001 gaccgcgtga tgagcgatga gatcgttctc gacgagggcg aggcgttcta tcttcaccca
    66061 ggagagctgg cgctggcggt gacgctggag tcggtgacgc tgccagccga tctggtgggc
    66121 tggctggacg ggcgttcctc actggcgcgt ctggggctga tggtgcacgt caccgcgcac
    66181 cgcatcgatc cgggctggtc tggttgcatt gtgctggagt tctacaactc cggtaagctg
    66241 ccgctggcgc tgcgtccggg catgttaatt ggtgcgctga gctttgagcc gctttccggc
    66301 ccggcggcgc gaccttacaa ccgccgtgaa gatgcgaaat atcgcaacca gcagggcgcg
    66361 gtagccagcc gaatcgataa agactaattc aggcccattg aggataccat gagacgattt
    66421 ctgacgacgc tgatgatact cctggtcgtg ctggtggccg ggttatctgc gttagtgttg
    66481 ctggtgaatc cgaatgattt ccgcgactat atggtcaagc aagttgctgc acgtagcggt
    66541 tatcaattgc agctcgacgg gccactgcgt tggcacgtct ggccgcagct tagtatcctc
    66601 tccgggcgaa tgtctctcac cgcccagggc gcaagccagc cactggttcg cgccgacaac
    66661 atgcgtctgg acgtggcgct tttaccacta ctgagtcatc aactgagcgt taagcaggtg
    66721 atgctaaaag gggcagtgat ccaactgacg ccgcagacgg aagcggtgcg cagtgaagac
    66781 gctccggttg caccgcgcga caataccttg ccggatctgt cagacgatcg cggatggtcg
    66841 tttgatatat ccagtcttaa ggtggcggac agcgtgctgg tgttccagca tgaagatgac
    66901 gagcaggtga caatccgcaa tattcgcctg caaatggaac aagatcccca acatcgtggt
    66961 tcatttgagt tctccgggcg ggttaatcgc gatcagcgcg atctcacgat atcccttaac
    67021 ggtacggtag atgcttctga ttatccgcat gatttaacgg cggctattga acaaattaac
    67081 tggcagttgc agggtgccga tttaccaaaa caaggtattc aggggcaggg gagtttccag
    67141 gcccagtggc aggagtcaca taaacgcctt tcatttaacc aaattagttt gaccgccaat
    67201 gatagtacgc taagcgggca agcacaggtt acgctgacag agaaaccgga atggcagctg
    67261 aggctgcaat tcccgcaact gaatcttgac aacctcatcc cgcttaatga aaccgcgaat
    67321 ggtgaaaacg gtgccgcgca gcaggggcag agccaatcaa cgttgccgcg cccggtcatt
    67381 tcttcgcgta ttgatgaacc ggcctatcag ggactgcaag gctttatggc tgatattttg
    67441 ttgcaggcca gtaacgtgcg ctggcgcgga atgaatttta cagatgttgc cacgcaaatg
    67501 accaacaagt cgggtttgct ggaaattact caactgcagg gcaaacttaa cggtggacag
    67561 gtttcactgc cgggcacgct ggacgcgaca tcaataaatc cgcggataaa cttccagcca
    67621 cggctggaaa acgttgagat tggtaccatt ctgaaggcgt ttaactatcc gatttcgttg
    67681 accggaaaaa tgtcactggc tggtgatttc tccggtgctg acatagatgc cgacgcattc
    67741 cgccacaact ggcaaggaca ggcacatgtc gaaatgaccg acacgcgcat ggaagggatg
    67801 aacttccagc agatgattca gcaagcggta gagcgtaatg gcggtgatgt gaaggccgct
    67861 gaaaactttg ataacgtgac gcggcttgac cgctttacca ccgatttgac gttgaaggat
    67921 ggcgtcgtga cgttaaacga catgcaaggt caatcgcctg tgctggcgct gacaggggaa
    67981 ggcatgttga atctggcaga tcaaacctgc gacacccagt ttgatattcg ggtcgtgggt
    68041 ggctggaacg gggaaagcaa actgattgat ttcctgaaag aaacgccagt accgctgcgg
    68101 gtttatggca actggcagca actcaattac agcctgcaag tggatcagtt actgcgcaaa
    68161 catctacagg acgaagcgaa acgtcgcctg aatgactggg ccgagcggaa taaagattcc
    68221 cgtaatggca aagatgtgaa gaagttgctg gagaagatgt aggttaccag gtgaccagtc
    68281 ctgtaggcct gataagacgc gtaaagcgtc gcatcaggcg ttcgtggcgg catcattata
    68341 ttctgaatat gtaagatcga aggctgttcc gttcacttga tgttcctcgc tactatggaa
    68401 cgctcaagcc ggtgaacgtg ccagggtggc tcaatctcca ccaccctggc aacccgggct
    68461 cccggcggta aatcgccgat ggcgcggttc cctcatctta ttcctttcgg ctattgggta
    68521 aaatgaccga taccatcaca cctcgtactc catctcgcca tccttacgca gcggtataat
    68581 ctgcaccttc tgcacgcgat ggctttctac ctgcaacgtt ttaagcaaat aatcccccac
    68641 ctgaacttct tcgccaggtt ttggaatacg ctgcaaatac tccatcaaca gcccggcaat
    68701 ggtgtgatat tcacgttttt catccagcgg cagcggcaca tattgcacca gatcctccag
    68761 cggcatatga ccattcgccg tccaggaacc gtccgcattc ttctgaatat catggcgggc
    68821 gtcgatctct tccacttcgt tcggtaagtt accggcaatg gtttcagtga cgtcacttaa
    68881 tgtcacaatc ccttccaccg agccaaactc atccaccaca aaagcaaagt gcgtgcgggc
    68941 attacggaac tgctccaggg caggtaacaa cggcaaggtt tccgggaaca ccagcggctg
    69001 gcgaatcaac acccgcaggt tgagcggttc gccgcgcaga gactgttgta gcaggtcgat
    69061 aacgtgaaca acaccgagca aatcttctgc gtcatcaccg tcggtaacca ccagccgcgt
    69121 atgctgattt cgctccagca actggcggat ttcttcttcc ggcgcgttga gatcgatatg
    69181 ctcaatatcg tggcgcgacg tcataatgct gctgacggta cgctggttaa gattaagtac
    69241 ccgctcaatc atccgccgtt cctgcggatt aaagatctgc tggttaccat gatccaccag
    69301 catggacgcg gtttcggcgt ccagttccgc atcttctttt tgcccgctaa gcaggcgcat
    69361 taccgcttcg gtagtccgct ggcgcagcgt ctggttagcg gaaagaaaac gtcgccggtt
    69421 aaagatagcc aactgattga gcgcctcgat catcaccgag aaaccaatgg cagcgtacag
    69481 gtagcctttc ggaatgacga agccgaaacc ttctgccacc aggctaaagc caatcatcaa
    69541 caggaagctt aagcagagaa taacgatcgt cgggtgactg ttaacaaatt gcgttaacgg
    69601 cttgctggcc atcaacatca ggctgatagc gataaccacg gcggccatca tgaccagtaa
    69661 atggtcgacc atcccgacgg cggtaatcac tgagtcgagt gagaaaatgg cgtccagtac
    69721 cacaatttgc gtcaccacgc cccagaactt cgcacctttg cgttgtgtgg gattattgct
    69781 gtctttccct tccagccgtt cgttcagctc catcgtggct ttgaacaaca ggaagaaacc
    69841 accaaacagc atgattaagt cgcgggcgct aaaggtaaac gagcggaagc tgaacagcgg
    69901 ttgagtcagg gtgaccagcc aggagattga cgccagcagt aacaggcgca ttaacattgc
    69961 cagcagcagc ccggtaaccc gtgcgcggtc acgctgcttc ggcggtagtt tttcggcgag
    70021 gatggcaata aagaccaggt tatcaatgcc gaggaccagt tcgatcacaa tcagcgtgat
    70081 taacccggcc cagatagacg gatcggcaat ccattccata gtaagtacaa aacctattcg
    70141 ttgtatgaca aatgtcacaa ttcgatcatg gataaatcgg ggcaaaaatg caatgttgca
    70201 gcactattta tctcatatta tttgagggta ataacctcac attatccctg aattaaaagt
    70261 ggtaataata aaacattatt taagaaatat cgcatgtgga ggcgcaatat taaatattgt
    70321 caacctaaag aaactcctaa aaaccatatt gaatgacact taatataatt cttaaaaata
    70381 gccaattacc gaattgttac cttgcctgct attccgttag ctgtaacact tcctcctgca
    70441 ttattggaga gccaatattc aattaccata atgtcacctt atattcggaa gttaatttcc
    70501 gcaataaccc tttctcatca acgattgtat gcagagaggg tttgaaaaat taagctaaat
    70561 cagtgtattg gtagctaaaa agccaggggc ggtagcgtgt ctggatgcct gaaagaccag
    70621 tctgaattat tctgtcaata gcctgcggat gaaatcaatt tttttaggac tgatgccagt
    70681 taaattttta ttcaattaac tgcaaggtaa atgtgcatgg cgacattgct attaatagtg
    70741 cacaggataa ttactctgcc aaagtgataa ataaacaatg atgaaatcca aaatgaaatt
    70801 gatgccatta ttggtgtcag taaccttgat aagcggttgc acagtacttc cgggcagcaa
    70861 tatgtcgacg atgggcaaag acgtcatcaa acagcaggac gctgatttcg atctcgacaa
    70921 aatggtgaat gtttatccgc tgaccccgcg cctgattgac caattacgcc cacgcccgaa
    70981 tgtagcgcgc cccaatatga cgctggaaag cgagatcgcg aattatcagt atcgcgtcgg
    71041 gccgggggac gttcttaatg tcaccgtctg ggatcacccg gaactcacca cgccagccgg
    71101 tcagtaccgc agctccagcg acaccggcaa ctgggtacag cctgacggca ctatgtttta
    71161 cccgtatatc ggcaaggttc acgtagtcgg gaaaacgctc gctgaaatcc gcagtgatat
    71221 taccgggcgc ttagcgacgt acatcgctga cccgcaggtg gacgttaata tcgccgcctt
    71281 ccgctcacaa aaggcctata tctccggtca ggtgaataaa tccggtcaac aggcgatcac
    71341 caatgtgcca ctgaccattc tcgatgccat caacgccgca ggtggcctga ccgacaccgc
    71401 tgactggcgc aacgtggtgc taacacacaa tggtcgtgaa gaacgcattt ctttgcaggc
    71461 gctgatgcaa aacggcgacc tcaatcagaa ccgactgctt tatcctggcg atattctcta
    71521 cgtgccgcgt aatgatgatc tgaaagtatt tgtgatgggt gaagttaaaa aacagagcac
    71581 cctgaaaatg gactttagcg gcatgaccct gactgaagcc ctgggcaatg ctgaaggcat
    71641 cgacatgacc acctccaacg ccagcggcat ctttgtcatt cgtccgttga aaggcgaggg
    71701 cgggcgtaac ggcaagattg ccaatatcta ccagctggat atgtccgatg ccacgtcgct
    71761 ggtgatggcg acagaattcc gcctgcaacc ttatgacgtg gtgtatgtca ccaccgcccc
    71821 ggtttcccgc tggaaccgtc tgatcaatca gttgctgcca actattagcg gtgtccgtta
    71881 catgacggat acagccagcg acattcataa ctggtaatcg tcatgtttaa caacatctta
    71941 gttgtctgtg tcggcaatat ttgccgttcc ccgacggcgg aacgcttact gcaacgttat
    72001 cacccggagc tgaaagtgga gtccgctgga ctcggcgcgc tggtcggtaa gggcgctgac
    72061 cctactgcta tcagcgtcgc cgcagaacat caactgtctc tggaaggtca ctgtgcccgg
    72121 caaatcagcc gccgcctgtg tcgcaactat gacctgattt tgaccatgga aaagcgccat
    72181 atcgaacgct tatgcgagat ggcacctgag atgcgcggca aagtgatgct gtttggtcac
    72241 tgggataacg aatgtgaaat ccccgatccg tatcgcaaaa gccgggaaac gtttgcagcg
    72301 gtgtacacat tacttgaacg gtctgcccgc cagtgggcgc aggcattgaa cgcagagcag
    72361 gtataagaat gacagaaaaa gtaaaacaac atgccgctcc ggtaacgggc agtgatgaaa
    72421 tcgatattgg tcgcctggtc ggcaccgtca ttgaagcgcg ctggtgggtg attggcatca
    72481 ccgctgtatt tgccctctgt gccgtggttt acaccttctt cgccacgccg atttatagtg
    72541 ccgacgcact ggtacaaatc gagcaaaaca gcggcaattc gttagtgcag gatatcggat
    72601 cggcattagc caacaaaccg cctgcatcgg acgccgagat ccagttgatt cgttcgcgcc
    72661 tggtgcttgg taaaacggtg gacgatctcg acctcgatat tgcgctgagc aaaaacacgt
    72721 tcccgatttt cggtgcgggc tgggatcgcc tgatgggacg tcagaacgaa acggtgaaag
    72781 tgaccacctt taaccgtccg aaagagatgg cggatcaggt gtttacgctt aatgtgctgg
    72841 acgataaaaa ctacactctg agcagcgatg gcggctttag cgcccgtggg caagcgggcc
    72901 agatgctgaa aaaagaaggc gtcacgctga tggttgaagc tattcacgct agcccgggca
    72961 gtgagtttac catcaccaaa tactccacgc tggggatgat caaccaactg caaaacagcc
    73021 tgacggtaac ggagaacggc aaagacgcag gcgtactgag cctgacttat accggtgaag
    73081 atcgtgaaca gatccgcgac attcttaaca gcatcgcccg taactatcag gaacaaaata
    73141 ttgagcgcaa atcggcggaa gcgtcgaaaa gtctcgcttt cctcgcgcaa cagttaccgg
    73201 aagtacgtag ccgcctcgat gttgccgaaa acaaactgaa tgccttccgt caggataaag
    73261 attctgttga tctgccgctg gaagcgaaag cggtgctcga ttcgatggtg aacatcgacg
    73321 cgcagttgaa cgaactgacc tttaaagagg cggaaatctc caagctgtac accaaagttc
    73381 acccggcgta tcgcacgctg ctggagaaac ggcaggcgct ggaagaagag aaagccaaac
    73441 ttaacggtcg cgtaacggcg atgccgaaaa cccagcagga gattgtgcgc ctgacccgcg
    73501 atgtcgagtc tggtcaacag gtctatatgc aactgctgaa taaagagcag gagctgaaaa
    73561 tcaccgaagc cagcaccgtc ggcgatgtgc gcattgttga cccggcaatc actcagcctg
    73621 gtgtgctaaa accgaagaaa gggctgatta tccttggggc gattattctt ggcctgatgc
    73681 tctctatcgt gggtgtgctg ctgcgctcgt tgtttaatcg tggcatcgaa agcccgcagg
    73741 tgctggaaga acacggtatc agcgtctatg ccagcatccc gttgtcggaa tggcagaaag
    73801 cgcgcgatag cgtcaaaacc atcaaaggga ttaaacgcta taaacagagc cagctactgg
    73861 cggtggggaa tccaaccgat ctggcgattg aagccatccg cagtctgcgt accagtctgc
    73921 acttcgcgat gatgcaggcg cagaacaatg tgttgatgat gaccggggtt agcccgtcaa
    73981 tcggtaaaac ctttgtctgc gctaacctgg cggcggtgat cagtcagacc aataaacggg
    74041 tgttgttgat cgactgcgat atgcgcaaag gctacaccca cgagctgttg ggcaccaata
    74101 acgtcaatgg cctgtcggaa attctgattg gtcagggcga tattactacc gctgctaaac
    74161 cgacctctat tgccaaattt gacctgatcc cgcgcggtca ggtgccgcca aatccttctg
    74221 agctgttgat gagcgaacgc tttgccgaac tggtgaactg ggcgagtaaa aactatgacc
    74281 tggtgttgat tgatacgccg ccgattctgg cagtgaccga tgcggcaatt gttggtcgtc
    74341 atgtcggaac cacgttaatg gtggcgcgtt atgcggtcaa cacattgaga gaagtggaaa
    74401 ccagtctgag ccgctttgag caaaatggta ttccggtgaa aggggtgatt ctgaactcca
    74461 tcttccgccg cgccagcgcg tatcaggatt atggctatta cgaatacgaa tataagtcgg
    74521 acgcgaaata acgacgcctg cattcgcacc gccccgtagg ccggataagg cgctcacgcc
    74581 gcatccggca agcaaaccag ctcataagcc gggagaacaa cctatgaaag ataatccgct
    74641 gatctcaatc tatatgccaa cctggaaccg ccaacaactg gcgattcggg caataaaatc
    74701 ggttttgcgc caggactaca gcaactggga gatgatcatc gtggatgatt gctccacctc
    74761 ctgggaacaa ctgcaacagt acgtcagcgc cctcaacgat ccgcgcatta cctacattca
    74821 caacgacatt aacagcgggg cgtgcgcggt acgtaaccag gcgattatgc tggcgcaagg
    74881 ggaatacatc accgggatcg atgatgatga tgaatggaca cccaaccgtc tgagcgtctt
    74941 cctcgcccat aaacagcaac tggtcactca cgcctttttg tacgctaacg attacgtttg
    75001 ccagggtgaa gtctattccc aaccggcaag tttgccgctg tatcccaaat cgccgtattc
    75061 acgccgcctg ttctataaac gcaatatcat tggcaatcag gtctttacct gggcgtggcg
    75121 tttcaaagag tgcttgtttg ataccgaact gaaagcggca caggattacg acatcttcct
    75181 gcggatggtg gtggagtacg gcgaaccgtg gaaagtggaa gaggcgacgc agatcctgca
    75241 tatcaatcac ggtgaaatgc agatcacctc gtcaccgaaa aaattctccg gatacttcca
    75301 cttttaccgc aagcacaaag acaaattcga ccgcgccagc aaaaaatatc agctgtttac
    75361 cctctaccag atccgcaata aacgcatgac ctggcgcact ttgctgacac tgctgtcggt
    75421 gcgtaacggc aaacgtctgg ctgatggtat tcgggggcgc taaagatgct ggaagatctg
    75481 cgtgccaaca gctggagttt acgcccgtgc tgcatggttc ttgcctatcg tgttgctcat
    75541 ttttgttcgg tgtggcgcaa aaagaacgtc ctcaacaatc tgtgggcggc cccgctgctg
    75601 gtgctgtatc gcattatcac cgaatgcttt ttcggttatg aaatccaggc agccgcaacc
    75661 attggccgcc gctttactat acatcacggt tacgccgtag tgatcaataa aaacgtggtt
    75721 gcgggggatg atttcaccat tcgccacggc gtcactatcg gcaatcgggg tgccgataac
    75781 atggcatgtc cacacattgg caacggcgtc gaactcggtg ccaacgtcat tattcttggt
    75841 gatatcacga ttggtaacaa cgtcaccgtg ggcgcgggca gcgtggtgct cgactctgtc
    75901 ccggacaacg cgctggtggt gggagaaaaa gcgcgagtga aggtaattaa atgaatattc
    75961 tgcaatttaa tgtgcgactg gcggaaggcg gggcggcagg tgtggcgtta gatctccacc
    76021 agcgtgcgct gcaacagggg ctggcgtcac attttgtcta cggctacggc aaaggcggca
    76081 aagagagcgt cagccatcag aactatccgc aggtcatcaa acatacgccg cggatgaccg
    76141 cgatggcgaa tattgccctg tttcgtctgt ttaatcgcga tctgttcggc aatttaaatg
    76201 agttatatcg caccattact cgcacaccgg gtccggtggt cctgcatttt catgtgctgc
    76261 acagctactg gttgaatctt aagagcgtgg tgcgcttttg cgaaaaagtg aaaaaccata
    76321 aaccggacgt cactctggtc tggacgctgc acgaccactg gagtgttacc ggacgctgcg
    76381 cctttaccga cggttgcgaa ggctggaaaa caggctgcca gaaatgcccg accttaaata
    76441 actatccgcc ggtgaagatt gatcgcgcac accaactggt ggcgggcaaa cgccagttat
    76501 tccgtgagat gctggcgctg ggctgtcagt ttatttcccc cagccagcat gtggctgacg
    76561 ctttcaatag cctgtacggt tcagggcgtt gccggattat caataatggc attgatatgg
    76621 caactgaagc gattctggcg gacttgcctc cggtgcgcga aacccagggc aagccgaaaa
    76681 tcgcggtggt ggcgcatgat ctgcgttacg acggcaaaac taaccagcaa ctggtacgcg
    76741 agatgatggc gctgggcgac aaaattgaac tgcatacctt tggtaagttc tcgccgttca
    76801 ccgctggcaa cgtggttaat cacggctttg aaaccgacaa acgtaagctg atgagcgcgc
    76861 tcaatcagat ggatgcgctg gtattcagtt ctcgcgtcga taactacccg ctgattttgt
    76921 gtgaggcgct atcgattggc gtgccggtga ttgccaccca tagcgatgcg gcgcgggaag
    76981 tgttgcaaaa atccggcggt aaaaccgtca gcgaagaaga ggtgctgcaa ctggtgcagt
    77041 taagcaaacc ggaaatcgcg caggcgatat ttggtaccac gctggctgag ttcagccaac
    77101 gcagccgcgc cgcctacagt ggacaacaga tgctggagga gtatgtcaac ttctatcaga
    77161 atctgtagct acctgttgct gccgctgatt tatctgctgg ttaacgtcaa aatcgcccag
    77221 cttggcgaaa gtttccccat caccatcgtc acttttttac ctgtcttgtt actgctgttt
    77281 ttagaacgca tcagcgttaa aaaattgatg attgccttag ggattggcgc gggactcacg
    77341 gcgtttaact atctgtttgg tcagtcgctg gatgccagta aatacgtcac ttcaactatg
    77401 ctgtttgtct atattgtgat cattattggc atggtgtgga gtattcgttt taaaacaatt
    77461 tcgccacaca accatcgcaa gatattacgt ttcttttatc tggtggtcgg gctggtggtg
    77521 gcgctggcgg cggtggagat ggcacaaatt atcctcaccg gtggcagcag tattatggag
    77581 tcgatttcga aatatctgat ttacagcaac agctatgtgc tgaatttcat taaattcggc
    77641 ggcaagcgca cgacagcact ttatttcgaa ccggcatttt tcgctctggc attaatctca
    77701 atttggctca gcatcaaaca gtttggtatc aaaacgccta aaacagatgc tatgattctc
    77761 gcagggataa tattatccgg atcgttttca ggggttatga cctttatcct gttttatttg
    77821 ctggagtggg catttcaata tctgaataaa gaggcgatta agaaaaagtt accgttagca
    77881 ttgatttctc tggctgtatt cctggttggt gtggtaatcg cgtttcctta tatttccacc
    77941 cgtctgggcg atttaggtac ggaaggatcg tcatcatatt atcgtattgt cggtccgctg
    78001 gtgatggtcg gttattcttt gacccatatt gacggtgtag tcagatttgg ctcactttat
    78061 gaatatgtcg catcattcgg aatatttaac ggtgcggatg tcggaaaaac catagacaat
    78121 ggtttgtatc tgctgattat ttatttttcc tggttcgcgg tgtttttatc actgtggtac
    78181 atggggaaag tgataaaaat gataatcaac gcttttggtg ataaccgcaa ttttcgcgtg
    78241 caattatatc tttttactcc ggtttcgctg ttttttaccg gttcgatatt tagcccggaa
    78301 tatgcatttt taatcgtctg tccgtttatt ttgcgaaaag cgttaaatat tacgaggtaa
    78361 gaataagaac atgttgctta gcataatcac tgtcgcgttt cgtaacctcg aagggatagt
    78421 caaaacacat gcctcgctgg cgcatctggc gcaggcggac gatatcagct tcgaatggat
    78481 tgttgtcgat ggcggttcca acgacggtac ccgtgagtat ctggaaaatc tcaatggtat
    78541 ctataaccta cgctttgtca gcgagccaga taacggtatc tacgacgcca tgaacaaagg
    78601 cattgcgatg gcacaaggca agttcgcgtt gtttctcaac tcgggcgata tttttcatca
    78661 ggatgccgca aattttgtcc gtaagttaaa aatgcaaaaa gataacgtga tgatcaccgg
    78721 cgatgcgctg ctggattttg gcgacgggca taaaattaaa cgtagcgcca aacctggctg
    78781 gtatatttat cacagcctgc ccgccagtca tcaggcgata tttttccctg tatccggctt
    78841 gaaaaaatgg cgttatgacc tggaatataa agtttcttcc gactacgcgc tggcagccaa
    78901 aatgtataaa gccggttatg catttaaaaa actcaatggc ctggtgtctg aattttccat
    78961 gggtggggta tctaccacca ataatatgga attgtgtgct gacgcgaaaa aagtccaacg
    79021 acaaatatta catgtgcctg gcttgtgggc tgaattatcc tggcatttac gccaacgtac
    79081 tacctcaaag acgaaagcct tatataacaa agtctgaata taaggaaaac caatgcaaga
    79141 tttaagcggt ttctcggtgc cgaaagggtt ccggggcggc aacgctatta aagtgcaatt
    79201 atggtgggca gtacaggcaa caatatttgc ctggtcgcca caagtattgt atcgctggcg
    79261 ggctttttta ttacgtttat tcggagcaaa aataggaaaa aacgtagtta ttcgtccgtc
    79321 agtaaaaatt acctatccgt ggaaattaac cttaggtgat tacgcgtggg tcggcgatga
    79381 cgtcaattta tataccctcg gtgaaataac cattggcgca cattcggtga tatcgcaaaa
    79441 aagttattta tgcaccggta gccacgacca tgcaagtcaa catttcacca ttaacgccac
    79501 gcctattgtg attggcgaga aatgctggct ggcaaccgat gtctttgttg ccccaggcgt
    79561 cacaatcggc gacggcaccg tcgtgggtgc acgaagcagt gtttttaaat cgcttccggc
    79621 aaatgtggtt tgccggggga atcccgcagt ggtgatacgc gaacgcgttg aaactgaata
    79681 aattcaaaaa tacagaggaa taatacatgt caaaagtcgc tctcatcacc ggtgtaaccg
    79741 gacaagacgg ttcttacctg gcagagtttc tgctggaaaa aggttacgag gtgcatggta
    79801 ttaagcgtcg cgcatcgtca ttcaacaccg agcgcgtgga tcacatttat caggatccgc
    79861 acacctgcaa cccgaaattc catctgcatt atggcgacct gagtgatacc tctaacctga
    79921 cgcgcatttt gcgtgaagta cagccggatg aagtgtacaa cctgggcgca atgagccacg
    79981 ttgcggtctc ttttgagtca ccagaatata ccgctgacgt cgacgcgatg ggtacgctgc
    80041 gcctgctgga ggcgatccgc ttcctcggtc tggaaaagaa aactcgtttc tatcaggctt
    80101 ccacctctga actgtacggt ctggtgcagg aaattccgca gaaagaaacc acgccgttct
    80161 acccgcgatc tccgtatgcg gtcgccaaac tgtacgccta ctggatcacc gttaactacc
    80221 gcgaatccta tggcatgtac gcttgtaacg gtattctctt caaccatgaa tccccgcgcc
    80281 gcggcgaaac cttcgttacc cgaaaaatca cccgcgcaat cgccaacatc gcccaggggc
    80341 tggagtcgtg tctgtacctc ggcaatatgg attccctgcg tgactggggc catgccaaag
    80401 actacgtaaa aatgcagtgg atgatgctgc aacaggaaca gccggaagat ttcgttatcg
    80461 cgaccggcgt tcagtactcc gtacgtcagt tcgtggaaat ggcggcagca cagctgggca
    80521 tcaaactgcg ctttgaaggc aggggtgttg aagagaaggg cattgtggtt tccgtcaccg
    80581 ggcatgacgc gccgggcgtg aaaccgggtg atgtgattat cgccgttgat ccgcgttact
    80641 tccgtccggc tgaagttgaa acgctgctcg gtgacccgac caaagcgcac gaaaaactgg
    80701 gctggaaacc agaaatcacc ctcagagaga tggtatctga aatggtggct aatgacctcg
    80761 aagcggcgaa aaaacactct ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg
    80821 agtcataagc atgagtaaac aacgcatttt tatcgctggt catcgcggga tggtcggttc
    80881 tgccatcaag cggcagctcg aacagcgcgg tgatgtggag ctggtattac gtacccgcga
    80941 tgaactgaac ctgctggaca gccgcgcggt gcatgatttc tttgccagcg aacgtattga
    81001 ccaggtctat ctggcggcgg cgaaagtggg cggcattgtt gccaacaaca cctatccggc
    81061 ggatttcatc taccagaaca tgatgattga gagcaacatc attcacgccg cgcatcagaa
    81121 cgacgtgaac aaactgctgt ttctcggatc gtcctgtatc tacccgaaac tggcaaaaca
    81181 gccgatggca gaaagcgaat tattgcaggg cacgctggag ccgaccaacg agccttacgc
    81241 cattgccaaa atcgccggga tcaaactgtg cgaatcttac aaccgccagt atggacgcga
    81301 ttaccgctca gtcatgccga ccaacctgta tggcccgcat gacaacttcc acccgagtaa
    81361 ttcgcatgtg atccctgcat tgctgcgtcg cttccacgag gcgacggcac agaatgcacc
    81421 ggacgtggtg gtatggggca gcggtacacc gatgcgtgaa ttcctgcacg tcgatgatat
    81481 ggcggcggcg agcattcatg tcatggagct ggcgcatgaa gtctggctgg agaacaccca
    81541 gccgatgctg tcgcacatta acgtcggcac gggcgttgac tgcactatcc gcgagctggc
    81601 gcaaaccatc gccaaagtgg tgggttacaa aggccgggtg gtttttgatg ccagcaaacc
    81661 ggatggcacg ccgcgcaaac tgctggatgt gacgcgcctg catcagcttg gctggtatca
    81721 cgaaatctca ctggaagcgg ggcttgccag cacttaccag tggttccttg agaatcaaga
    81781 ccgctttcgg gggtaatgat gtttttacgt caggaagact ttgccacggt agtgcgctcc
    81841 actccgcttg tctctctcga ctttattgtc gagaacagtc gcggcgagtt tctgcttggc
    81901 aaaagaacca accgcccggc gcagggttac tggtttgtgc cgggagggcg cgtgcagaaa
    81961 gacgaaacgc tggaagccgc atttgagcgg ctgacgatgg cggaactggg gctgcgtttg
    82021 ccgataacag caggccagtt ttacggtgtc tggcagcact tttatgacga taacttctcc
    82081 ggcacggatt tcaccactca ctatgtggtg ctcggttttc gcttcagagt atcggaagaa
    82141 gagctgttac tgccggatga gcagcatgac gattaccgct ggctgacgcc ggatgcactg
    82201 ctcgccagtg acaatgttca cgctaacagc cgcgcctatt ttctcgctga gaagcgtgcc
    82261 ggagtacccg gattatgaaa atactggtct acggcattaa ctactcgccg gagttaaccg
    82321 gcatcggcaa atacactggc gagatggtgg aatggctggt agcacaaggt catgaggtgc
    82381 gggtcattac cgcaccgcct tactacccgc agtggcaggt gggcgagaac tattccgcct
    82441 ggcgctacaa acgagaagag ggggccgcca cggtgtggcg ctgcccgctg tacgtgccaa
    82501 aacagccgag caccctgaaa cgcctgttac atctcggcag ttttgccgtc agcagtttct
    82561 ttcctctgat ggcgcaacgt cgctggaagc cggatcgcat tatcggcgtg gtgccaacgc
    82621 tgttttgcac gccgggaatg cgcctgctgg cgaaactctc tggtgcgcgt accgtgctgc
    82681 atattcagga ttacgaagtg gacgccatgc tggggctggg ccttgccgga aaaggcaaag
    82741 gcggcaaagt tgcacagctg gcaacggcgt tcgaacgtag cggactgcat aacgtcgata
    82801 acgtctccac gatttcgcgc tcgatgatga ataaagccat cgaaaaaggc gtggcggcgg
    82861 aaaacgtcat cttcttcccc aactggtcgg aaattgcccg ttttcagcat attgcagatg
    82921 ccgatgttga tgcccttcgt aaccagcttg gcctgccgga taacaaaaaa atcattcttt
    82981 actccggcaa tattggtgaa aagcaggggc tggaaaacgt tattgaagct gccgatctcc
    83041 tgcgcgatga accgctgatt tttgccattg tcgggcaggg cggcggcaaa gcgcggctgg
    83101 aaaaaatggc gcaacagcgt ggtctgcgca acatgcaatt ttttccgctg caatcgtatg
    83161 acgctttacc cgcactgctg aagatgggcg attgccatct ggtggtgcaa aaacgcggcg
    83221 cggcagatgc cgtattgccg tcgaaactga ccaatattct ggcggtaggc ggtaacgcgg
    83281 tgattactgc agaagctcac acagaactgg gacagctttg cgaaaccttt ccgggcattg
    83341 cggtttgcgt tgaaccggaa tcggtcgagg cgctggtggc ggggatccgt caggcgctcc
    83401 tgctgcccaa acacaacacg gtggcacgtg aatatgccga acgcacgctc gataaagaga
    83461 acgtgttacg tcaatttata aatgatattc ggggataatt atggcgcagt cgaaactcta
    83521 tccagttgtg atggcaggtg gctccggtag ccgcttatgg ccgctttccc gcgtacttta
    83581 ccccaagcag tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca ccatctgccg
    83641 cctgaaaggc gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc accgctttat
    83701 tgtcgcggaa cagctgcgtc aactgaacaa actcaccgag aacattattc tcgaaccggc
    83761 agggcgtaac actgcacctg ccattgcgct ggcggcgctg gcggcaaaac gtcatagccc
    83821 ggagagcgac ccgttaatgc tggtgctggc ggcggatcat gtgattgccg atgaagacgc
    83881 gttccgtgcc gccgtgcgta atgccatgcc atatgccgaa gcgggcaagc tggtgacctt
    83941 cggcattgtg ccggatctac ctgaaaccgg ttatggctat attcgtcgcg gtgaagtgtc
    84001 ggcgggtgag caggatacgg tggcctttga agtggcgcag tttgtcgaaa aaccgaatct
    84061 ggaaaccgct caggcctatg tggcaagcgg cgaatattac tggaacagcg gtatgttcct
    84121 gttccgcgcc ggacgctatc tcgaagaact gaaaaaatat cgcccggata ttctcgacgc
    84181 ctgtgaaaaa gcgatgagcg ccgtcgatcc ggatctcgat tttattcgcg tggatgaaga
    84241 agcgtttctg gcctgcccgg aagagtcggt ggattacgcg gtcatggaac gtacggcaga
    84301 tgccgttgtg gtaccgatgg atgcgggctg gagcgatgtc ggttcctggt cttcattatg
    84361 ggagatcagc acccacaccg cggaaggcaa cgtttgccac ggcgatgtga ttaatcacaa
    84421 aaccgaaaac agctatgtgt acgccgaatc tggcctggtc accaccgtcg gggtgaaaga
    84481 tctggtagtg gtgcagacca aagatgcggt gctgattgcc gaccgtaacg cggtgcagga
    84541 tgtgaaaaaa gtggtcgagc agatcaaagc cgatggtcgc catgagcatc gggtgcatcg
    84601 cgaagtctat cgcccgtggg gcaaatatga ctctatcgac gcgggcgacc gctaccaggt
    84661 gaaacgcatc accgtgaaac cgggcgaagg cttgtcggta cagatgcacc atcaccgtgc
    84721 ggaacactgg gtggtggtcg cgggaacggc aaaagtcact attgacggtg atatcaaact
    84781 gcttggtgaa aacgagtcca tttatatacc gctgggggcg acgcactgcc tggaaaaccc
    84841 agggaaaatt ccgctcgatt taattgaagt gcgctccggc tcttatctcg aagaggatga
    84901 tgtggtgcgt ttcgcggatc gctacggacg ggtgtaaacg tcgcatcagg cgctgtttgt
    84961 cggatgcggc gtgaacgcct tatccgacct acggttcgga tttgtaggcc tgataagacg
    85021 cggcagcgtc gcatcaggcg ctggctgtcg gatgcggcgt gaacgcctta tccgacctac
    85081 ggttcgggtt tgtagtcctg ataagacgcg gcagcgtcgc atcaggcaat gaatgcgaaa
    85141 ccgcggtgta aataacgaca aaaacaaaat tggccgtttc ggtcagggcc aactattgcc
    85201 tgaaaaaggg taacgacatg aaaaaattaa cctgctttaa agcctatgat attcgcggga
    85261 aattaggcga agaactgaat gaagatatcg cctggcgcat tggtcgcgcc tatggcgaat
    85321 ttctcaaacc gaaaaccatt gtgttaggcg gtgatgtccg tctcaccagc gaaaccttaa
    85381 aactggcgct ggcaaaaggt ttacaggacg caggcgtcga tgtgctggat attggcatgt
    85441 ccggcaccga agagatctat ttcgccacgt tccatctcgg cgtggatggc ggcatcgaag
    85501 ttaccgccag tcataatccg atggattaca acggcatgaa gctggtgcgc gagggggctc
    85561 gcccgatcag cggtgatacc gggctgcgcg acgtccagcg cctggcagaa gccaacaact
    85621 ttcctcccgt tgatgaaaca aaacgcggtc gctatcagca aatcaatctg cgtgacgcct
    85681 acgttgctca tctgttcggt tatatcaacg tcaaaaacct cacgccgctc aagctggtga
    85741 tcaactccgg gaacggcgcg gcgggtccgg tagtggacgc tattgaagcc cgctttaaag
    85801 ccctcggtgc acccgtggaa ttaatcaaag tgcataacac gccggacggc aatttcccca
    85861 acggtattcc taacccgctg ttgccggaat gccgcgacga cacccgcaat gcggtcatca
    85921 aacacggcgc ggatatgggc attgcttttg atggcgattt tgaccgctgt tttctgtttg
    85981 acgaaaaagg gcagtttatc gagggctact acattgtcgg cctgctggca gaagcgttcc
    86041 tcgaaaaaaa tcccggcgcg aagatcatcc acgatccacg tctctcctgg aacaccgttg
    86101 atgtggtgac cgccgcgggc ggcactccgg tgatgtcgaa aaccggacac gcctttatta
    86161 aagaacgtat gcgcaaggaa gacgccatct acggtggtga aatgagcgcc caccattact
    86221 tccgtgattt cgcttactgc gacagcggca tgatcccgtg gctgctggtc gccgaactgg
    86281 tgtgtctgaa aggaaaaacg ctgggcgaac tggtgcgcga ccgaatggca gcgtttccgg
    86341 caagcggtga gatcaacagc aaactggcgc aacccgttga ggcgattaac cgcgtcgaac
    86401 agcactttag ccgtgaggtg ctggcggtgg atcgcaccga tggcatcagc atgacctttg
    86461 ccgactggcg ctttaacctg cgctcctcca acaccgaacc ggtggtgcgg ttgaatgttg
    86521 aatcgcgcgg tgatgtgccc ctgatggaag cgcgaacgcg aactctgctg acgttgctga
    86581 acgagtaatg tcattacttt ccccctcacc ctgacgctct actgcctgcg gagagaagtg
    86641 agcacggaac tttcttctcc ccctcgcccc tttggggaga gggccgtggt gaggggcgag
    86701 gcgacaatag aacaggaaca acgatgacaa atctaaaaaa gcgcgagcga gcgaaaacca
    86761 atgcatcgtt aatctctatg gtgcaacgct tttcagatat caccatcatg tttgccggac
    86821 tatggctggt ttgcgaagtg agcggcctgt cattcctcta catgcacctg ttggtggcac
    86881 tgattacgct ggtggtgttc cagatgttgg gcggcatcac cgatttttat cgctcatggc
    86941 gcggtgttcg ggcagcgaca gaatttgctc tgttgctaca aaactggacg ttaagcgtga
    87001 ttttcagcgc cggactggtg gcgttcaaca atgatttcga cacgcaactg aaaatctggc
    87061 tggcgtggta tgggctgacc agcatcggac tggtggtttg ccgttcgtgt attcgtattg
    87121 gggcgggctg gctgcgtaat catggctata acaagcgcat ggtcgccgta gcgggggatt
    87181 tagccgccgg acaaatgctg atggagagct tccgtaatca gccgtggtta gggtttgaag
    87241 tggtgggggt ttaccacgac ccgaaaccgg gcggcgtttc taacgactgg gcgggtaacc
    87301 tgcaacagct ggtcgaggat gctaaagcag gcaagattca taacgtctat atcgcgatgc
    87361 aaatgtgcga cggcgcgcga gtgaaaaaac tggtccatca actggcggac accacctgtt
    87421 cggtgctgct gatccccgac gtctttacct tcaacattct ccattcacgt ctcgaagaga
    87481 tgaacggcgt tccggtggtg ccgctgtacg atacgccgct ttccggggtt aaccgcctgc
    87541 taaaacgtgc ggaagatatt gtgctggcga cgcttattct gctgctgatc tccccggtgc
    87601 tgtgctgtat tgcgctggcg gtaaaactca gttcaccagg accggttatt ttccgccaga
    87661 ctcgctatgg catggatggc aaaccgatca aagtgtggaa gttccgttcc atgaaagtga
    87721 tggagaacga caaagtggtg actcaggcga cgcagaacga tccgcgcgtc accaaagtgg
    87781 ggaactttct gcgccgcacc tcgctggacg aattgccgca gtttatcaat gtgctgactg
    87841 gcgggatgtc gattgtcggt ccgcgtccgc atgcggtggc gcataacgaa cagtatcgcc
    87901 agctcattga aggctacatg ctgcgccata aggtgaaacc gggcattacc ggctgggcgc
    87961 agattaacgg ctggcgcggc gaaaccgaca cactggagaa aatggaaaaa cgcgtcgagt
    88021 tcgaccttga gtacatccgc gaatggagcg tctggttcga tatcaaaatc gttttcctga
    88081 cggtgttcaa aggtttcgtt aacaaagcgg catattgata tgagcttacg tgaaaaaacc
    88141 atcagcggcg cgaagtggtc ggcgattgcc acggtgatca tcatcggcct cgggctggtg
    88201 cagatgaccg tgctggcgcg gattatcgac aaccaccagt tcggcctgct gactgtgtcg
    88261 ctggtgatta tcgcgctggc agatacgctt tctgatttcg gtatcgctaa ctcgattatt
    88321 cagcgaaaag aaatcagcca ccttgaactc accacgctgt actggctgaa cgtcgggctg
    88381 gggcttgtgg tgtgcgtggc ggtgtttttg ttgagtgatg ccattggtga cgtgctgaat
    88441 aacccggacc tggcaccgtt gattaaaaca ttgtcgctgg cgtttgtggt gatcccccac
    88501 gggcagcagt tccgcgcgtt gatgcagaag gagctggagt tcaacaaaat cggcatgatc
    88561 gaaaccagcg cggtactggc gggcttcact tttacggtag ttagcgccca tttctggccg
    88621 ctggcgatga ccgcgatcct cggttatctg gtcaatagtg cggtaagaac gctgctgttt
    88681 ggctactttg gccgcaaaat ttatcgcccc ggtctgcatt tctcgctggc gtcggtggca
    88741 ccgaacttac gctttggtgc ctggctgacg gcggacagca tcatcaacta tctcaacacc
    88801 aacctttcaa cgctggtgct ggcgcgtatt ctcggcgcgg gcgtggcagg gggatacaac
    88861 ctggcgtaca acgtggccgt tgtgccgccg atgaagctga acccaatcat cacccgtgtg
    88921 ttgtttccgg cattcgccaa aattcaggac gataccgaaa agctgcgtgt taacttctac
    88981 aagctgctgt cggtagtggg gattatcaac tttccggctc tgctggggct gatggtggtg
    89041 tcgaataact ttgtaccgct ggtctttggt gagaagtgga acagcattat tccggtgctg
    89101 caattgctgt gtatggtggg tctgctgcgt tcggtgggta acccaattgg ttcgctgctg
    89161 atggcgaaag cgcgggtcga tatcagcttt aaattcaacg tattcaaaac atttctgttt
    89221 attccggcga ttgttattgg tgggcagatg gagggcgcaa ttggcgtcac gcttggcttc
    89281 ctgctggtgc agattatcaa caccattctg agttacttcg tgatgattaa accggttctt
    89341 ggttccagtt atcgccagta catcctgagt ttgtggctgc cgttttatct ctcgctgccg
    89401 acgctggtgg tcagttatgc gttgggcatt gtgctgaaag ggcaactggc gttggggatg
    89461 ctgctggcag tgcaaatagc cgcgggcgtg ctggcgtttg tggtgatgat tgtgctgtcg
    89521 cgccatccgc tggtggtgga agtgaagcgt cagttttgtc gcagcgaaaa aatgaaaatg
    89581 cttttacggg cggggtgagt tgctgttttc ggttccgaca acggttgccg gatgcggcgt
    89641 aaaacgcctt atccgtccta cggttctgtg ctcgggactt gtaggcctga taagacgcgc
    89701 cagcgtcgca tcaggcactg aatgccggat gcggcgtgaa cgccttatcc gtcctacggt
    89761 tctgtgctcg gatcttgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    89821 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    89881 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    89941 tacttgatgc catcaacatc ctcaatccac acgctgaagt ggacgtgatg agccgctatc
    90001 cggtcagttc ctcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaaa
    90061 tgaaacaaca caacagcgcg gcgggcgttg tcgggcgcgt taaaaaagtc ctccgtcgcc
    90121 gctatcaaca ccaggtattg ctctcacgcg tcaccgacac tggcaagctg cgtaatatcg
    90181 ccatcgccca gggattcacc gacttcgtgc gcctgctctc aggttacgac gccattattc
    90241 aggtcggagg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    90301 gcacgtttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    90361 atgagcaatt taaccaactg gcgaactacg tttttggtca ctgcgacgcg ctgatcatgc
    90421 gcgaatcggt cagccttgat ctgatgaaac gcagcaatat cactaccgca aaagtggaac
    90481 atggcgtcga taccgcgtgg ctggtcgatc accacacaga agatttcacc gccagctatg
    90541 ccgttcaaca ctggctggac gtcgccgcac aacagaaaac ggtggcaatt accctgcgcg
    90601 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    90661 ccggggtggt caatcgcatt ctcgatgaag ggtatcaggt gattgcgctc tccacctgta
    90721 cgggcatcga cagctataac aaagacgacc gcatggtggc gctcaacctg cgccagcata
    90781 tcagcgatcc tgcccgttat cacgtagtga tggatgaact caacgatctg gaaatgggca
    90841 aaattctcgg tgcctgtgaa ctcaccgtcg gtacgcgtct gcactccgcc attatctcaa
    90901 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    90961 agcagctggg actaccggag atgtcaattg atatccgtca tttattagac ggcagcctgc
    91021 aagcgatggt tgcggatacc ttaggccagc ttccggcgct gaacgcacga cttagcgaag
    91081 ccgtcagtcg tgagcgtcag acgggaatgc agatggtgca atctgtgctt ga
//
LOCUS       DAFATA010000005        90073 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFATA010000005 DAFATA010000000
VERSION     DAFATA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.005"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            743..2005
                     /locus_tag="LGJ20_000555"
     CDS             743..2005
                     /locus_tag="LGJ20_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HBK2384680.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDS
                     TNKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFK
                     TTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYN
                     IQFAFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEK
                     IIETIESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRF
                     VVSRVLKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKY
                     DMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAA
                     KIIKTARYVIMKLSENYPYKGVYEKCLV"
     gene            2293..3168
                     /locus_tag="LGJ20_000556"
     CDS             2293..3168
                     /locus_tag="LGJ20_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-1"
                     /protein_id="HBK2384681.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
     gene            complement(3215..3547)
                     /locus_tag="LGJ20_000557"
     CDS             complement(3215..3547)
                     /locus_tag="LGJ20_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBK2384682.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSAFAV"
     gene            4086..5240
                     /locus_tag="LGJ20_000558"
     CDS             4086..5240
                     /locus_tag="LGJ20_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBK2384683.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            5391..6215
                     /locus_tag="LGJ20_000559"
     CDS             5391..6215
                     /locus_tag="LGJ20_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK2384684.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            6300..7502
                     /locus_tag="LGJ20_000560"
     CDS             6300..7502
                     /locus_tag="LGJ20_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBK2384685.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            7562..8146
                     /locus_tag="LGJ20_000561"
     CDS             7562..8146
                     /locus_tag="LGJ20_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK2384686.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            8541..8999
                     /gene="traH"
                     /locus_tag="LGJ20_000562"
     CDS             8541..8999
                     /gene="traH"
                     /locus_tag="LGJ20_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HBK2384687.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            8996..9814
                     /gene="traI"
                     /locus_tag="LGJ20_000563"
     CDS             8996..9814
                     /gene="traI"
                     /locus_tag="LGJ20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HBK2384688.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            9811..10959
                     /gene="traJ"
                     /locus_tag="LGJ20_000564"
     CDS             9811..10959
                     /gene="traJ"
                     /locus_tag="LGJ20_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBK2384689.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            10956..11246
                     /locus_tag="LGJ20_000565"
     CDS             10956..11246
                     /locus_tag="LGJ20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK2384690.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            11261..11812
                     /locus_tag="LGJ20_000566"
     CDS             11261..11812
                     /locus_tag="LGJ20_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBK2384691.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            11901..15668
                     /locus_tag="LGJ20_000567"
     CDS             11901..15668
                     /locus_tag="LGJ20_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK2384692.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STASAPSPAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            15686..16033
                     /locus_tag="LGJ20_000568"
     CDS             15686..16033
                     /locus_tag="LGJ20_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK2384693.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            16030..16722
                     /locus_tag="LGJ20_000569"
     CDS             16030..16722
                     /locus_tag="LGJ20_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBK2384694.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            16733..17716
                     /gene="traN"
                     /locus_tag="LGJ20_000570"
     CDS             16733..17716
                     /gene="traN"
                     /locus_tag="LGJ20_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HBK2384695.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            17719..19008
                     /gene="traO"
                     /locus_tag="LGJ20_000571"
     CDS             17719..19008
                     /gene="traO"
                     /locus_tag="LGJ20_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBK2384696.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            19008..19712
                     /gene="traP"
                     /locus_tag="LGJ20_000572"
     CDS             19008..19712
                     /gene="traP"
                     /locus_tag="LGJ20_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HBK2384697.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            19712..20239
                     /gene="traQ"
                     /locus_tag="LGJ20_000573"
     CDS             19712..20239
                     /gene="traQ"
                     /locus_tag="LGJ20_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBK2384698.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            20290..20694
                     /gene="traR"
                     /locus_tag="LGJ20_000574"
     CDS             20290..20694
                     /gene="traR"
                     /locus_tag="LGJ20_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HBK2384699.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISTISIG"
     gene            20758..20946
                     /gene="traS"
                     /locus_tag="LGJ20_000575"
     CDS             20758..20946
                     /gene="traS"
                     /locus_tag="LGJ20_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HBK2384700.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            20930..21730
                     /gene="traT"
                     /locus_tag="LGJ20_000576"
     CDS             20930..21730
                     /gene="traT"
                     /locus_tag="LGJ20_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HBK2384701.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            21820..24864
                     /gene="traU"
                     /locus_tag="LGJ20_000577"
     CDS             21820..24864
                     /gene="traU"
                     /locus_tag="LGJ20_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HBK2384702.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            24864..25478
                     /gene="traV"
                     /locus_tag="LGJ20_000578"
     CDS             24864..25478
                     /gene="traV"
                     /locus_tag="LGJ20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HBK2384703.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            25445..26647
                     /gene="traW"
                     /locus_tag="LGJ20_000579"
     CDS             25445..26647
                     /gene="traW"
                     /locus_tag="LGJ20_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HBK2384704.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            26676..27260
                     /gene="traX"
                     /locus_tag="LGJ20_000580"
     CDS             26676..27260
                     /gene="traX"
                     /locus_tag="LGJ20_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HBK2384705.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            27357..29525
                     /locus_tag="LGJ20_000581"
     CDS             27357..29525
                     /locus_tag="LGJ20_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBK2384706.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGILPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            29599..30249
                     /gene="excA"
                     /locus_tag="LGJ20_000582"
     CDS             29599..30249
                     /gene="excA"
                     /locus_tag="LGJ20_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HBK2384707.1"
                     /translation="MQMVKTINRYPAWWTLYYALRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(30321..30530)
                     /locus_tag="LGJ20_000583"
     CDS             complement(30321..30530)
                     /locus_tag="LGJ20_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBK2384708.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            30922..31098
                     /locus_tag="LGJ20_000584"
     CDS             30922..31098
                     /locus_tag="LGJ20_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384709.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            31757..32008
                     /locus_tag="LGJ20_000585"
     CDS             31757..32008
                     /locus_tag="LGJ20_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384710.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(32080..32232)
                     /locus_tag="LGJ20_000586"
     CDS             complement(32080..32232)
                     /locus_tag="LGJ20_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBK2384711.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            32524..33732
                     /gene="trbA"
                     /locus_tag="LGJ20_000587"
     CDS             32524..33732
                     /gene="trbA"
                     /locus_tag="LGJ20_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBK2384712.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            33751..34821
                     /gene="trbB"
                     /locus_tag="LGJ20_000588"
     CDS             33751..34821
                     /gene="trbB"
                     /locus_tag="LGJ20_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HBK2384713.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            34814..37105
                     /locus_tag="LGJ20_000589"
     CDS             34814..37105
                     /locus_tag="LGJ20_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HBK2384714.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(37142..39841)
                     /gene="nikB"
                     /locus_tag="LGJ20_000590"
     CDS             complement(37142..39841)
                     /gene="nikB"
                     /locus_tag="LGJ20_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HBK2384715.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG"
     gene            complement(39852..40184)
                     /gene="nikA"
                     /locus_tag="LGJ20_000591"
     CDS             complement(39852..40184)
                     /gene="nikA"
                     /locus_tag="LGJ20_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HBK2384716.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            40418..40753
                     /locus_tag="LGJ20_000592"
     CDS             40418..40753
                     /locus_tag="LGJ20_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBK2384717.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTASKKTDSAR
                     KDGIDMNNN"
     gene            complement(40838..41686)
                     /locus_tag="LGJ20_000593"
     CDS             complement(40838..41686)
                     /locus_tag="LGJ20_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBK2384718.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREAQDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFIGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     SLPPRV"
     gene            42035..42232
                     /locus_tag="LGJ20_000594"
     CDS             42035..42232
                     /locus_tag="LGJ20_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384719.1"
                     /translation="MQGAPHPARLKKNDGATMACGGHWHTTPAQVKTEKTARHLQSKK
                     VKFLHFFTLIFRADLVVMVIK"
     gene            42266..42517
                     /locus_tag="LGJ20_000595"
     CDS             42266..42517
                     /locus_tag="LGJ20_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2384720.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(42548..42718)
                     /locus_tag="LGJ20_000596"
     CDS             complement(42548..42718)
                     /locus_tag="LGJ20_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384721.1"
                     /translation="MTIAERLIQKGFEKGALEVAREIACRLRDMGWTPERIQEATGLS
                     GEELKKLFPDEQ"
     gene            complement(42780..43721)
                     /locus_tag="LGJ20_000597"
     CDS             complement(42780..43721)
                     /locus_tag="LGJ20_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBK2384722.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRQRDMLMCLDNLVRALQDIQDEEQITVLFNYL
                     LNGSEHVTVEFLQTLAQRLPQHEDSIMTLAERLKQEGIQQGIQQGIQQGIQQGVQQGA
                     LQKAREIARELRNAGMPAAQICQLTGLSEAELKNITH"
     gene            complement(43786..44052)
                     /locus_tag="LGJ20_000598"
     CDS             complement(43786..44052)
                     /locus_tag="LGJ20_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384723.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(44144..44578)
                     /locus_tag="LGJ20_000599"
     CDS             complement(44144..44578)
                     /locus_tag="LGJ20_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBK2384724.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(45307..45807)
                     /locus_tag="LGJ20_000600"
     CDS             complement(45307..45807)
                     /locus_tag="LGJ20_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK2384725.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLATFDDERDFFAACRSL
                     HQDEADPELMFQDYEGFPGNMASECHINWAYVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(46270..46866)
                     /locus_tag="LGJ20_000601"
     CDS             complement(46270..46866)
                     /locus_tag="LGJ20_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384726.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(46863..47582)
                     /locus_tag="LGJ20_000602"
     CDS             complement(46863..47582)
                     /locus_tag="LGJ20_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBK2384727.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(47579..48013)
                     /gene="psiB"
                     /locus_tag="LGJ20_000603"
     CDS             complement(47579..48013)
                     /gene="psiB"
                     /locus_tag="LGJ20_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBK2384728.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(48068..50026)
                     /locus_tag="LGJ20_000604"
     CDS             complement(48068..50026)
                     /locus_tag="LGJ20_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK2384729.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAGSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRALAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVKDNTKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQMQKKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            complement(50085..50318)
                     /locus_tag="LGJ20_000605"
     CDS             complement(50085..50318)
                     /locus_tag="LGJ20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBK2384730.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            complement(50376..50903)
                     /locus_tag="LGJ20_000606"
     CDS             complement(50376..50903)
                     /locus_tag="LGJ20_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK2384731.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNGITRYITEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            51272..51340
                     /locus_tag="LGJ20_000607"
                     /pseudo
     CDS             51272..51340
                     /locus_tag="LGJ20_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(51673..51864)
                     /locus_tag="LGJ20_000608"
     CDS             complement(51673..51864)
                     /locus_tag="LGJ20_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384732.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(51861..52283)
                     /locus_tag="LGJ20_000609"
     CDS             complement(51861..52283)
                     /locus_tag="LGJ20_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBK2384733.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVVALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(52330..52632)
                     /locus_tag="LGJ20_000610"
     CDS             complement(52330..52632)
                     /locus_tag="LGJ20_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBK2384734.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHIVNGANWFDRTVSA
                     DACGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(53173..53943)
                     /locus_tag="LGJ20_000611"
     CDS             complement(53173..53943)
                     /locus_tag="LGJ20_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384735.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSCGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(53994..54428)
                     /locus_tag="LGJ20_000612"
     CDS             complement(53994..54428)
                     /locus_tag="LGJ20_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBK2384736.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            complement(54440..54661)
                     /locus_tag="LGJ20_000613"
     CDS             complement(54440..54661)
                     /locus_tag="LGJ20_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384737.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(54662..55345)
                     /locus_tag="LGJ20_000614"
     CDS             complement(54662..55345)
                     /locus_tag="LGJ20_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBK2384738.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(55422..55733)
                     /locus_tag="LGJ20_000615"
     CDS             complement(55422..55733)
                     /locus_tag="LGJ20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384739.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(55730..56632)
                     /locus_tag="LGJ20_000616"
     CDS             complement(55730..56632)
                     /locus_tag="LGJ20_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBK2384740.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            57050..57298
                     /locus_tag="LGJ20_000617"
     CDS             57050..57298
                     /locus_tag="LGJ20_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBK2384741.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            57295..57732
                     /gene="umuD"
                     /locus_tag="LGJ20_000618"
     CDS             57295..57732
                     /gene="umuD"
                     /locus_tag="LGJ20_000618"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBK2384742.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            57732..59006
                     /locus_tag="LGJ20_000619"
     CDS             57732..59006
                     /locus_tag="LGJ20_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK2384743.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(59008..59424)
                     /locus_tag="LGJ20_000620"
     CDS             complement(59008..59424)
                     /locus_tag="LGJ20_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBK2384744.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(59417..60397)
                     /locus_tag="LGJ20_000621"
     CDS             complement(59417..60397)
                     /locus_tag="LGJ20_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBK2384745.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            complement(60811..61119)
                     /locus_tag="LGJ20_000622"
     CDS             complement(60811..61119)
                     /locus_tag="LGJ20_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HBK2384746.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(61206..61850)
                     /locus_tag="LGJ20_000623"
     CDS             complement(61206..61850)
                     /locus_tag="LGJ20_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK2384747.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(62030..62809)
                     /locus_tag="LGJ20_000624"
     CDS             complement(62030..62809)
                     /locus_tag="LGJ20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBK2384748.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(62811..63224)
                     /locus_tag="LGJ20_000625"
     CDS             complement(62811..63224)
                     /locus_tag="LGJ20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384749.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            63756..64682
                     /locus_tag="LGJ20_000626"
     CDS             63756..64682
                     /locus_tag="LGJ20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384750.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADTDRVITDLASHARKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDEELASYLDAKVMEQRYQSHFITRWNQEYQK
                     LLGISTEEELRRLVLNNTPFLQVRADQVLKGWKNIPRGISLTFSLFAEIAGRDRETID
                     QAWKNIFYSQLREKKHRFYQDIEAIRALKKLPALTGDSWTRDGITVRIYRPENYARGA
                     WRVTLSLPENYATQMWNIPFPELEYRLFTADPGYSALISAEPDRWDKAFRFVDGVCEL
                     HLYTNGVEEDHNPTPLGDVAQALINVVEENLL"
     gene            64727..64981
                     /locus_tag="LGJ20_000627"
     CDS             64727..64981
                     /locus_tag="LGJ20_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384751.1"
                     /translation="MKGRQSRYVTGGESFAEIARLPSGAVVRLCLNTGLEDALREASK
                     SLKSAFTRSGRKCRLSAGTAQGPFTGRRQGVATHLFVSVL"
     gene            65480..65824
                     /locus_tag="LGJ20_000628"
     CDS             65480..65824
                     /locus_tag="LGJ20_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HBK2384752.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKVFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(66000..67001)
                     /locus_tag="LGJ20_000629"
     CDS             complement(66000..67001)
                     /locus_tag="LGJ20_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HBK2384753.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            67352..67609
                     /locus_tag="LGJ20_000630"
     CDS             67352..67609
                     /locus_tag="LGJ20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384754.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            67609..68199
                     /locus_tag="LGJ20_000631"
     CDS             67609..68199
                     /locus_tag="LGJ20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384755.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(68219..68567)
                     /locus_tag="LGJ20_000632"
                     /pseudo
     CDS             complement(68219..68567)
                     /locus_tag="LGJ20_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            68686..69033
                     /locus_tag="LGJ20_000633"
     CDS             68686..69033
                     /locus_tag="LGJ20_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HBK2384756.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
     gene            complement(69051..70931)
                     /locus_tag="LGJ20_000634"
     CDS             complement(69051..70931)
                     /locus_tag="LGJ20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B"
                     /protein_id="HBK2384757.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AINSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     NIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWAFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(71210..72556)
                     /locus_tag="LGJ20_000635"
     CDS             complement(71210..72556)
                     /locus_tag="LGJ20_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384758.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            72910..73512
                     /locus_tag="LGJ20_000636"
     CDS             72910..73512
                     /locus_tag="LGJ20_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBK2384759.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            73568..74062
                     /locus_tag="LGJ20_000637"
     CDS             73568..74062
                     /locus_tag="LGJ20_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384760.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            74062..74328
                     /locus_tag="LGJ20_000638"
     CDS             74062..74328
                     /locus_tag="LGJ20_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384761.1"
                     /translation="MLTGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(74805..75278)
                     /locus_tag="LGJ20_000639"
     CDS             complement(74805..75278)
                     /locus_tag="LGJ20_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384762.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(75395..76243)
                     /locus_tag="LGJ20_000640"
     CDS             complement(75395..76243)
                     /locus_tag="LGJ20_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBK2384763.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NSYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(76283..76564)
                     /locus_tag="LGJ20_000641"
     CDS             complement(76283..76564)
                     /locus_tag="LGJ20_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2384764.1"
                     /translation="MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(76561..76830)
                     /locus_tag="LGJ20_000642"
     CDS             complement(76561..76830)
                     /locus_tag="LGJ20_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384765.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            77188..78333
                     /locus_tag="LGJ20_000643"
                     /pseudo
     CDS             77188..78333
                     /locus_tag="LGJ20_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
     gene            complement(78509..78811)
                     /locus_tag="LGJ20_000644"
     CDS             complement(78509..78811)
                     /locus_tag="LGJ20_000644"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384766.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(78898..79713)
                     /gene="sul2"
                     /locus_tag="LGJ20_000645"
     CDS             complement(78898..79713)
                     /gene="sul2"
                     /locus_tag="LGJ20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBK2384767.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            80043..80219
                     /locus_tag="LGJ20_000646"
     CDS             80043..80219
                     /locus_tag="LGJ20_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBK2384768.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(80401..81156)
                     /locus_tag="LGJ20_000647"
                     /pseudo
     CDS             complement(80401..81156)
                     /locus_tag="LGJ20_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
     gene            complement(81209..81913)
                     /locus_tag="LGJ20_000648"
     CDS             complement(81209..81913)
                     /locus_tag="LGJ20_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBK2384769.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(82115..82903)
                     /locus_tag="LGJ20_000649"
     CDS             complement(82115..82903)
                     /locus_tag="LGJ20_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBK2384770.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(83034..83507)
                     /locus_tag="LGJ20_000650"
     CDS             complement(83034..83507)
                     /locus_tag="LGJ20_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HBK2384771.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            83665..84678
                     /gene="intI1"
                     /locus_tag="LGJ20_000651"
     CDS             83665..84678
                     /gene="intI1"
                     /locus_tag="LGJ20_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBK2384772.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            84881..85231
                     /locus_tag="LGJ20_000652"
     CDS             84881..85231
                     /locus_tag="LGJ20_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBK2384773.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            85407..85967
                     /locus_tag="LGJ20_000653"
     CDS             85407..85967
                     /locus_tag="LGJ20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2384774.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            85971..88385
                     /locus_tag="LGJ20_000654"
                     /pseudo
     CDS             85971..88385
                     /locus_tag="LGJ20_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            88462..88734
                     /locus_tag="LGJ20_000655"
     CDS             88462..88734
                     /locus_tag="LGJ20_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384775.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            88715..89923
                     /locus_tag="LGJ20_000656"
     CDS             88715..89923
                     /locus_tag="LGJ20_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBK2384776.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
BASE COUNT        22774 a        23868 c        22667 g        20764 t
ORIGIN      
        1 gtgccaatcc ggtcggtggt cagggggtaa taaaattaat tactcagcat gtaattggta
       61 caaatcatcc gtagcaatga atcactttat cggcggtaag agtggtggtt cgatctatta
      121 caagccgatt caatgtccga ctggatacat tatgactgga acacgcatgt acggtattgg
      181 tgatggtgtt gatgaggaac atgtcgatgc ctactgttgc ccctttaatt aagctccagc
      241 accaggaaga ttgaggcatg tgactgatat ggtcccacca gactcataat ttccccaagc
      301 ataattactc acataccaaa cgttgttttg gtcggaatat acctgttgaa aaccaccaaa
      361 gggtgagcca ttcaggtaag tccatgagca atatgcatgg accccgagcc ttaaattacg
      421 agtgttttta gctatgttgt aggtctgcgt tgtgaattga attttaggag cgccccaggt
      481 accggattgg cactggccta gtgccaatcc ggtacctgga aatcatcatc agcgtcgata
      541 tggacgaata taaaaaccta gattctacgt cagtacttca aaaagcataa tcaaagcctt
      601 gataaatatg cattccttcg aaattcagct ttcacccatt gggtgaaaga aaagtgctca
      661 aaaatatgtt aaattatcag cttttatgac tcgatatatg gtaaaataat agtaagaaaa
      721 gtagtaaaaa ggggttctaa ttatgattaa taaaattgat ttcaaagcta agaatctaac
      781 atcaaatgca ggtctttttc tgctccttga gaatgcaaaa agcaatggga tttttgattt
      841 tattgaaaat gacctcgtat ttgataatga ctcaacaaat aaaatcaaga tgaatcatat
      901 aaagaccatg ctctgcggtc acttcattgg cattgataag ttagaacgtc taaagctact
      961 tcaaaatgat cccctcgtca acgagtttga tatttccgta aaagaacctg aaacagtgtc
     1021 acggtttcta ggaaacttca acttcaagac aacccaaatg tttagagaca ttaattttaa
     1081 agtctttaaa aaactgctca ctaaaagtaa attgacatcc attacgattg atattgatag
     1141 tagtgtaatt aacgtagaag gtcatcaaga aggtgcgtca aaaggatata atcctaagaa
     1201 actgggaaac cgatgctaca atatccaatt tgcattttgc gacgaattaa aagcatatgt
     1261 taccggattt gtaagaagtg gcaatactta cactgcaaac ggtgctgcgg aaatgatcaa
     1321 agaaattgtt gctaacatca aatcagacga tttagaaatt ttatttcgaa tggatagtgg
     1381 ctactttgat gaaaaaatta tcgaaacgat agaatctctt ggatgcaaat atttaattaa
     1441 agccaaaagt tattctacac tcacctcaca agcaacgaat tcatcaattg tattcgttaa
     1501 aggagaagaa ggtagagaaa ctacagaact gtatacaaaa ttagttaaat gggaaaaaga
     1561 cagaagattt gtcgtatctc gcgtactgaa accagaaaaa gaaagagcac aattatcact
     1621 tttagaaggt tccgaatacg actacttttt ctttgtaaca aatactacct tgctttctga
     1681 aaaagtagtt atatactatg aaaagcgtgg taatgctgaa aactatatca aagaagccaa
     1741 atacgacatg gcggtgggtc atctcttgct aaagtcattt tgggcgaatg aagccgtgtt
     1801 tcaaatgatg atgctttcat ataacctatt tttgttgttc aagtttgatt ccttggactc
     1861 ttcagaatac agacagcaaa taaagacctt tcgtttgaag tatgtatttc ttgcagcaaa
     1921 aataatcaaa accgcaagat atgtaatcat gaagttgtcg gaaaactatc cgtacaaggg
     1981 agtgtatgaa aaatgtctgg tataataaga atatcatcaa taaaattgag tgttgctctg
     2041 tggataactt gcagagttta ttaagtatca ttgcagcaaa gatgaaatca atgatttatc
     2101 aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt gtgagaagca gtctaaattc
     2161 ttcgtgaaat agtgattttt gaagctaata aaaaacacac gtggaattta ggttagacta
     2221 taaatagaaa aaggcgtttt gacagactat tcatgttgtt gttaattcgt ctcttccaga
     2281 ataaggaatc ccatggttaa aaaatcactg cgtcagttca cgctgatggc gacggcaacc
     2341 gtcacgctgt tgttaggaag tgtgccgctg tatgcgcaaa cggcggacgt acagcaaaaa
     2401 cttgccgaat tagagcggca gtcgggagga agactgggtg tggcattgat taacacagca
     2461 gataattcgc aaatacttta tcgtgctgat gagcgctttg cgatgtgcag caccagtaaa
     2521 gtgatggccg tggccgcggt gctgaagaaa agtgaaagcg aaccgaatct gttaaatcag
     2581 cgagttgaga tcaaaaaatc tgacttggtt aactataatc cgattgcgga aaagcacgtc
     2641 gatgggacga tgtcactggc tgagcttagc gcggccgcgc tacagtacag cgataacgtg
     2701 gcgatgaata agctgatttc tcacgttggc ggcccggcta gcgtcaccgc gttcgcccga
     2761 cagctgggag acgaaacgtt ccgtctcgac cgtaccgagc cgacgttaaa caccgccatt
     2821 ccgggcgatc cgcgtgatac cacttcacct cgggcaatgg cgcaaactct gcgtaatctg
     2881 acgctgggta aagcattggg tgacagccaa cgggcgcagc tggtgacatg gatgaaaggc
     2941 aataccaccg gtgcagcgag cattcaggct ggactgcctg cttcctgggt tgtgggggat
     3001 aaaaccggca gcggtgacta tggcaccacc aacgatatcg cggtgatctg gccaaaagat
     3061 cgtgcgccgc tgattctggt cacttacttc acccagcctc aacctaaggc agaaagccgt
     3121 cgcgatgtat tagcgtcggc ggctaaaatc gtcaccaacg gtttgtaata gcggaaacgg
     3181 aatggggaaa ctcattccgt ttttgtttat cgccttagac ggcaaaagcg ctgtcgccca
     3241 cctgcgcttg cgcataccag gccataagct ccgtggttcc tggttctcct tccgctggcg
     3301 cccagtgcgc atagtcatcg gcagccacgg gttgatagcc accgtgtttt acttcaaaaa
     3361 ttatgccacc ggtatccagc gacagcacgg catgccaggt tcctgcggcc atctccagca
     3421 ccgtacaggt ttcccccaat atcgcccgat gggtgacggt accccgatcg tcaaaattca
     3481 gcaccacgaa acgacccctt aatggcaaca gtagttcgaa ggtgtgaggg tgtcggtgcg
     3541 ggcgcacgta ggttcctggt aaaaacattt acgttatatc ccaaaaacac acaggtctta
     3601 gggaggttta aactgtgtat taacacctat cgtattgacg ggcgtgaaat ggcagaaaca
     3661 gaagtcgttc ctattgacat gcctgactct aacggcgaga tgacttggca agccaaaaac
     3721 tacacacagt attcgtccta ctttatgaag attacttgct tgaaatagct tattggcaaa
     3781 tggcatatac agttattgaa ccattctgat cttcggttgt atcgcacgca gccgaccatg
     3841 agttattggc tgatgggaat gacctggtaa gccatatgta accagttcgt gaggtgcata
     3901 ttccgccgcc acctgtaaca cgttttccag ttggacaagt agctgttgct ccaggaaagc
     3961 gccaacctgt ggcctgtgct gtagcaattt gaagctcacc agatcctacc tttctccagg
     4021 taccggattg gcacgcttgc agagaatgat tgttggcatt attttttgcc tcttttgagg
     4081 ttagcatgcc gtctccacgc atccgtaaaa tgtccctgtc acgcgcactg gataagtacc
     4141 tgaaaacagt ttctgttcac aagaaagggc atcaacagga gttttaccgg agcaatgtta
     4201 tcaagcgata tcccattgcc cttcggaata tggacgaaat aacaaccgtt gatattgcta
     4261 catacagaga cgttcgttta gcagaaatca acccccgaac gggtaaaccc attacaggta
     4321 atactgtacg tcttgaactc gcccttctgt catctctgtt caatattgct cgtgttgaat
     4381 ggggaacctg tcgtactaac ccggttgaac tggttcgcaa gccgaaagta tcctcgggac
     4441 gagatcgccg gctaacgtct tcagaagaac gtcgcctttc tcgctatttc cgcgaaaaaa
     4501 atctgatgtt gtatgtcatt ttccatcttg cccttgagac agccatgcgg cagggcgaaa
     4561 tactggcctt acgttgggag cacattgatt tgcgccacgg tgtggctcat ttacctgaaa
     4621 ccaaaaacgg tcactcacgg gatgttcctc tgtccagacg tgcccgtaac tttcttcaga
     4681 tgatgcccgt taatctccac ggcaatgttt ttgattacac cgcatccggc tttaaaaatg
     4741 cctggagaat agccacacaa cgacttcgca tcgaggacct gcattttcac gatctacggc
     4801 atgaagcaat aagccgcttc ttcgaactgg gtagcctgaa tgtaatggag attgctgcaa
     4861 tatcaggaca tcgttccatg aatatgctga aacggtatac tcatcttcgt gcatggcaac
     4921 tggtcagtaa gcttgatgcc cgccggcggc agacacaaaa agtggcagca tggtttgtgc
     4981 cgtatcctgc ccatatcacg accatcgatg aagaaaatgg gcagaaagcg catcgtattg
     5041 agatcggtga ttttgataac cttcacgtca ctgccacaac gaaagaggaa gcagttcacc
     5101 gcgccagtga ggttttgttg cgtacactgg ccattgcagc acagaaaggc gaacgtgtcc
     5161 catctcccgg agcgttacct gttaacgacc ctgactacat tatgatttgc cctctgaacc
     5221 cgggcagcac cccgctgtaa acatcattca aaacacatac agaaaggcga gttgcaacaa
     5281 ctcgccaaac acattcgatc tgcatgatcg aaattattcg tttcaccaga aaaaacaccg
     5341 aagatacaat aaccacatga gctgaaaaga ctttttcagt cggaggaaca atgagactga
     5401 atacgaccgg tattgcggcc cgaatgatgc tctcgctgga caaaaaagca atcggcgaaa
     5461 aactcatcaa cggtcgtcag ataaacccga cacctctgca ggtccgttac ccccaagggg
     5521 taagagaagt tctggggata atgagcgaac aattagccat atccaccgct gatttaaccc
     5581 gaatcctgct tgaggatgct cttcataata tgtttatgcc tgcggataac accacaggaa
     5641 acataataag cagaattgaa tacatcatgc tgtcgcatga tataaatgcg ccattactgg
     5701 caacattgct gtcgccatgg aatatccgct ccacggttat acaggacccg gccagacttg
     5761 cagactatct ttcagctgac gctctcgaac atcttgctga atgttttaat ctcaatccgg
     5821 actggctgaa tggccatgaa aactatccga tagcgttgtc cggtgaatgg ccagatactg
     5881 ctgataattt cagaatgctt attagtgata gcagcaacac tgaagttatt ttctggcata
     5941 gtttcccctt cgcaggaaac acgaaaagag aatattgcgg ggtaatattg agacagaaaa
     6001 aagaaataaa tggctctgta atatatccgg cactatcatt atcaccaaca cttctgaacg
     6061 atgaaaaaag aaaatggtta actgaatata caaccaggca gaatacaaca atgtctttgc
     6121 gacgcgtcac gttgagacct ggccttgccg ggaaccttat tacagggcaa atactacctg
     6181 ttagcttatt taatacgtct ttattaccct ggtaaatacc agatagtacc aaagtaaaaa
     6241 ttacggcaca tccgtgtcat agcgttgttt tgtctaaatt ctgataaaca ggaaattaaa
     6301 tgatgaagaa aaatcatatt acccgcacca tcattgcatc tgctgtatta ttttccttta
     6361 acgctgcagc agcgacgtca tattttgaag cgcgtaacga tgcaatgggt ggtaccgggg
     6421 tagcatcttc ccattatggt gtggcacctc tggcaaaccc ggctttactg acaaagcaca
     6481 acagcaatga tgatttcagc cttcttctgc cgtctgttgg ggcacaagtt gctgatcctg
     6541 atgatgtatc aaataaagct gatgatgtta aagatgactg ggatttgttt gacagtgctg
     6601 tagataacca gcatggcgta caacaggcag cggcaaatct taaacatcgt ctgcaggaat
     6661 tccgcaatat caatgcagat gctcaggtag gtgtatcagc tgtagcagcc atggcaaatg
     6721 acactctgcc gtttgccctt atggtcaagt cttacggaac tgtcagcgta aacggaaaag
     6781 taaacgatgc agacctggat tatctggaca aagttgccaa tggaacaatt accgacgtcg
     6841 ataagaatgc cctgacctca cgcgcattcg ggcgtgcagc tgtaattacc gacgtcggaa
     6901 tttcatttgc caaagaactg gaaacggccg gacaaaaatg gtctcttggc gtcacaccaa
     6961 aatatcagcg tgtggacctg tttaactaca acgtaacggt tcgtgattac gacaaagacg
     7021 attttgacgg tgacaagtac cacaacacga aaaacggatt caacgccgat attggtgctt
     7081 atacagacct gaatgacaac tggactgtcg gtcttgttgc ccagaacatc attccacgca
     7141 gtattgatac taaagtcgtc aatggcttta aagaaacctt caaagttcgt ccgcaggcaa
     7201 ccgccggtgt atcctggcat aatgacctgt ttaccactgc tcttgacatt gatctgactc
     7261 ctgccagtgg cttcacttct gacagcaaac gtcagtttgc aagtgttggt gctgaattta
     7321 acgcatggaa atgggcgcaa ttgcgtgccg gttatcgtca gaacatggca tcaaactccg
     7381 gaagcgcctt caccgctggc gttggtatct ctccatttga cgtcgtacat atcgatgttt
     7441 ccggcctggt aggaactgac cacgattacg gtgcaatggc acaacttcag ttcactttct
     7501 gattactgtc cccgcccccg gcactaaacc cgggggtatt tttatattca gtttaaataa
     7561 catgatgaaa ccacgttctt cttattcaaa aacagcattc attcttttat tttctgtttt
     7621 cctggtagcg gcagtgacaa aagcaaaaag ctcgcttccc gatatcaccc tggaacaggc
     7681 aaaagaaatt aatgctgata acaccgttat tttccttttc cgccatggag aacgttgcga
     7741 ccgttcagat atgccatgct attcggataa aagcggaatc actattaccg gtacagagaa
     7801 agcacagcag gaaggtatta agttcgcaac aatcttcagt gaatatgata tttactccag
     7861 caatgcggta agaaccatac aaactgcaaa attcttctcc ggtaaagaac ccgtcgttat
     7921 ggacagttta tcagactgca ataatgatct ttataagacg cttgaatcaa tcgcaagaga
     7981 atctcataaa aggaacatag taataatgac acataatcat tgtttatcat ttctggccag
     8041 agataggctc ggcaagaaat tcaaaccggc gtaccttgat gccctgatca tgcattacga
     8101 tggtacccgg ctcatacttg acggaaaata caacaaagag gcatagagga tatttgaatt
     8161 tacataaatt ctgatatact cagtaagata caagagtgta cgggcacatg tgcctgtctg
     8221 aattgcaggt tatccggacg agggccggga actgtttcag ggacaagcgc cggtacgttc
     8281 gattcataaa tcgtcgagtc atccatcatg gatcgattcg gcgcggagcg ttaaagatta
     8341 cgctcatatt cgttttatgt ctgacgacac atcaaccagc cagtatcagg aataatcacc
     8401 ttcagtaaac tatgaggcta tttacctggc cacactggca tttatttccg gtaacgcaag
     8461 tatagccgga aacaaacatc atcctgtctg taccggagca ccggtatatc ccattctgat
     8521 ttaactgctc aggaattatt atgaaccgta ttatacctgc ggggtgtctt gccgcccttt
     8581 ttctggctgg ctgcaactcc ccttctcagc atactcctgt tccgcgtaac tacggtgctg
     8641 cctctgatgt ggcggttacc cggcacactc agtacacatt atgggacaaa ggtgtatttt
     8701 ccggccaact tccggtttca acggggtctc atcttctgac agctaacagc gatcgccttt
     8761 cctttgactg ggaaggtgat gcaattgaat tactgaacga actggccaga gtcaggggga
     8821 tgcagtttaa ctacaatggt gtccgcctgc cgttaccagt caaccttcac gtccgggata
     8881 tgacgttttc caatacgttg agacttattg aagcccagac agcctggaga gcaacaatcc
     8941 atcaatatcc gggcttgtta caggtcagct tcatgcagcc ggagaaccgg aaaaaatgaa
     9001 agcactcacc tctctccttt tgccagtaat actgcttact ggttgttctt cttcccctac
     9061 catctcgcca ggtggtgtca atacgccacc acctgacatt aatgcatggc ttaaaccacg
     9121 aagtgaaaag ccggatagtc tgtcagaaac acgctggaaa atgctgacag acgcaggtaa
     9181 aacgcttgga tttcgtggtg gcaaggctca gcgttcatgg gagctgattc aggcgctcaa
     9241 cgcccgggaa agcacactta atgccttata cgatttccgg ccactgatta gcccggaagg
     9301 ctggctacca ccggttattg atgaagcaca ggacgtagct catatcacgc cagatcaaat
     9361 ccggacctct tcccgggtct ggaccatcat tcgccctgaa cgctttgtca gcaatccacc
     9421 gggctggcgt gactggcttc tccgggggct ttcaacaaca gccactccgg gtacagaagg
     9481 cagcgtcgtt ccagaagaca gcgtgcaacg aaaagtgtgg gaaaccgcat tacggcaggg
     9541 atggcaggaa ggaagacaaa acgccgacct gacgcttgaa gccaaccaga aaaccctcac
     9601 ccgtgattac cggggcatga tgctgtactc acttctctgg cgtcagggca tgatcacccg
     9661 tccggatgtt tctgatcaaa tgcaaaccgt tacaggtgac gggaaaaaac tggtcactgg
     9721 agatcgtgtt cgtcgcctta aaaatcatgc ggaattcaat cttcagaaat ctcactggcg
     9781 tccgcttata ggcactgaag gaggctcccg atgaatattc ctgatgagtt tggtcttttt
     9841 ccgtttcagc gtttcactgc agatgaacta cggcatttct ttgtctggtg tgccgcacat
     9901 aaagtcagtg atgtggatct gaccggcggg tcacctgtat ctgtcagtcg ctttggccga
     9961 cgggtgcgtt gttccagtgc aacgttacca accacactca tgtcctccct gattgatgaa
    10021 ctgttcggtc gggaagtcat tccccgcgtt ctggcaggca atccggttga caggacgata
    10081 cagattaacg gggatgcatc cgggcgctat ggtcttaaac gcggagaacg catccggtta
    10141 cgctctcacc tcattcaggg cacatctggt gcggaggaga aagcaatctc aataaccatg
    10201 cgtgtcatcc cgacagaaat accggacatt ctctccatga acattgaacc agacctgctt
    10261 gaagcaatgg tctgcaaaag cggtctggga tttgtatgtg gtgaaaccgg gtcaggaaaa
    10321 tcaacgctgt gttcagcact gtaccgttac attatggaca atttccccga cgccaaaatc
    10381 gtgacatatg aagacccggt ggaatatatc ctgggaaatg aaaacgatct gctcccaccg
    10441 catcaggcag aaatcgggcg tgatgtggtc agttttgctg ccggactgcg ttctgctgta
    10501 cgacgtaatc ccgaaattat cggtgttggt gagatcaggg ataacgaaac cgccgacgca
    10561 gcggtacagg caggcaacac cgggcattac tgcctgagca ccatgcatac gaaatctcct
    10621 ggtgaaacgc tggcacgtct tcttgggctg tttccccctg tcatcaggga ttcgatggcc
    10681 tgggctgtcc tgtcactgtt gcagttcatt ctggttcagg ttctggtcag aacaaatgac
    10741 ggtggtcgta aagccgtccg ggaatatatc gtcattaacg atgagcttcg cgacaatctt
    10801 tccggaatgc cgcatgcaga gtgggggcat cacattgatg ccataatccg gcaggagaag
    10861 cgccgtatcc gggaccagat tctggaaatg tatatccgga atgaagttga ccgaagagaa
    10921 gcgattcttt ttatcccgcc aggggagtta cggtcatgaa aagagaaggt ttctggcgat
    10981 atgcaacacc gccagtcaac atccagggcg tacctctgcc gttcaccatg atttatctgt
    11041 ttatatgccc ctttccctcc aaaacaacct tctggatttg caccggcatc attttatttt
    11101 tcgttattct cgaccgttca ggctggactg tcaggactat ctgtacacgc attttcagca
    11161 ttctgagagg ggccatcgcc tccggtcgtc catggtggta tcgccaccat acggaatcac
    11221 cacgagactg gactggcctc gattaaattc tggagtcttt atgtcaggta aaaaaactat
    11281 cgttactctg ctccgggtat cgctactggc atgcccgtta ctgttcacga caccatcgtt
    11341 tgcaatgatc gacacgccat ccgtgaaggt cggcttttct cctgaaggaa gcgcatccgc
    11401 tctggtgctt gacacaatca acagtgcgga aagctccatc aggatgatgg catattcctt
    11461 caccgacccg gatgtaatgc atgcgctggc aaaagcgaaa aaacgcggag tggacgtccg
    11521 tattgttgtt gatgacaagg ggaatacgaa ccgggcaagt caggaagcga tgaaatatat
    11581 caacctgctg gacattcctc tccggactgt agatgccttc cccatccatc acgacaaagt
    11641 catcattgtt gacggaaaca cggttgaaac tggctcctat aacttctctc gtgctgctgc
    11701 acgcaagaat tcagagaatg tcgttgtgct caagaatatg ccggatgtag ccgcacaata
    11761 tcttgaacac tggcaggatc gctggaataa gggtacagac tggaaaccct gacaaacatt
    11821 tctcccccga ttttctatcc ccccagcccc cccggcttcc ggcggtggtt tttttccgtc
    11881 ttttttcagg agaagcactc atgccgtctg tatcccgcta ccgcacctgg cttgccgtgc
    11941 ctgcagatga aatagaagat ctcaaaaaag cacatccccc gatgaacggg cacacgcccg
    12001 tcatctggga caaggagcac aaactctggt tcgcccgccc gggggcggac ctgtccaggc
    12061 tggaccgctg gctgccccgg ccacaggacg tctccatgaa cggcagtgat ccggtcaccg
    12121 agtttgccca ggttctggag aatgccggac tggttctgaa agaactgccg gtcatggacg
    12181 ggaaaatcca ccgtgtcccg accgccgatg acaaaaaagg acaaaaaagt ggcgcatatc
    12241 gcggattcct ggacggcaga cctgccggct ggtacaggga ttatcgcagc gcggatgatt
    12301 cgccgatcac ctggaccttc tccggcggag aacagacaga tccccgcgca agactccacc
    12361 tgaaagcgca ttcaatgcaa cgcagggagg acgcggagcg ggaactgaaa gcacagtaca
    12421 accgacaggc cgcgtatgcc cgccgctatg tcaacaaatg gccgcaggcc acagcacatg
    12481 aatacctgac ccggaaaggc attcaggctg caccgggggt gcgggtaaac aacaaaaatg
    12541 aactggttat tcccttcagt aaccgtaacg gcgcgatacg ctcttaccag cgtattccgg
    12601 tcaccggggg gaaggatgcc cgcatcctga aagattcaga gaaaaccggt aactggtttg
    12661 cgctgggcac accgcgcaac ggtcagccgg tactgtttgc cgagggctat gccactgcgg
    12721 cctccctgca cgaagccacg ggccttccgg tactgatgac ggtggatgga ggcaacatga
    12781 ttgccgttgc cgaaaatgcc cggcaaaagt ggacgcaaag cccctttatc ttctgtgccg
    12841 acaacgatca tgcaatccgg gttaacaaag gtatcgtcag tgcaacaaaa gccgcagaac
    12901 tgacaggagg aacggtcatc tttcctgcgt tcaccgatgc cgaaaaagcg caggggctga
    12961 ccgatttcaa tgacctggac gcaagccggg gacgggccgc ttttcagcat gtcatcaacg
    13021 cccagttaga acacatcggg gtttccaccc ccaacagtaa caccccggaa atccgcgagg
    13081 cactggtgat cggcaacctc gtttttacac cggtccacac tgaggaaaaa accatgactc
    13141 cgacagaata tcctgaaaca tcacccgata cagggcacag ccatgaccag ggaccttctc
    13201 tgccgtccgc cacgcagcag gaacaaccag ctgcttccag cagtaacatt gctgatgaaa
    13261 cacagtcatt taccagtcac gccactgaaa ataacggcaa agatgagcgg cacgctgata
    13321 acgttcaggc gcaaacggaa agctctgtag ttcaggactc ccctgcagaa tcaccggcgt
    13381 ccccggaaag tacaacggca tctgcccctg atgaacccgc tcatccggcg gaacctccgg
    13441 aaaaagttgt ctcggtggcc acagataaag actggcgtga atttgaagcc gaactgtcac
    13501 aaccggaaaa aaacgagtca caacaggaat ccggcacttc acctgaaata ccggcccctg
    13561 ctgccgcccc cgcatcccct gaagacagtc cctcatctga aaccatgaat gaatcagccg
    13621 agccatcacc ttcccctgat gcagtcaaag aggaaatgac cgtgatgacg gagcctgatg
    13681 acaaacaaag caccggaata tccccggata ctccccggga agaggcggtc aaccatgtaa
    13741 ctgaagacat tccccttcag ccagagccgt tccttcctga gtcagatggc cccgaagaat
    13801 acgaaaacta cagcgcatat caggagctga tgaacaatga aaatcctgag catctgcagg
    13861 aaaacagtga tatgccgtcg caaccggaca cagaccacgt tcaggcagat gagtcagaac
    13921 ttcaggccac aacagatact gcagaaccgg ctgtccggga tgaacccact ccacagcaac
    13981 ctgttcagag cacatcacct tctgataata cttcatcgtt tctcgacaaa gcgcgtggct
    14041 tttttacgcg taaaaaaacg gattcacagg cacatgagaa cagtgatccc acgccctcag
    14101 cggaaaccac gacgactcca ccgatacctg acagtattgt ctacgccccg gaacgttctg
    14161 atgcaccgat atcgctcaac atggacgaaa ttcttaaaga gcttgactgg gaaaaacgag
    14221 cagacagaac cgtcctgtac aaactggatg gcaaacctgc cttcattgac cgggtaaacc
    14281 ggctggaaat ggtcaacggt gccagcaatg acgatcgcag cgttctggcc gcacttgctg
    14341 tggccaccaa attttatggg ggcgtcatag agctgacggg cagtgatgcc tttaagcaaa
    14401 aagcaatgca gttgattatc gagcacaaca tcgatgtccg gatgaaattg ccagatcaac
    14461 gggctgaact agaaaaatta cgcaaggaaa tggctgtcag taaggatgca gttgtcaccc
    14521 atcagccaac accggaactt aaccggaata ccccagaaca accggctgtc cctgaccccg
    14581 ttcaggaaaa agaagccaca caatccccgg cgtcccctgt tgcccctgaa gcatcaaccg
    14641 tcagcaccgc ctctgcacca tcaccggctg aaccaggcaa aacggcagat gtggcaccag
    14701 acgaagaacc accaggaaaa cttcgcccgg gagagagcgt gactgccgta ctgcacaatt
    14761 ttggtcgtgc tgaatatgca cccggcaaag gagagagttt ctttgttgag cttaaaaacc
    14821 gcagtggcag caaactctac tggggggaac aactggaaag tctggtcaaa aaccatcaga
    14881 aaggtgacgt ggtgacactg acacttcaga accgcgaaca attcatcctc ccgggagagc
    14941 aaaaagcacg tttccggaat aagtggtcca tggagtcagt taccaacggg atctcggtat
    15001 cacatgataa tccggataaa ggtcagcgta ttcaggcgat tccggtcgaa acctttatga
    15061 aagtggctgc gcaaatcagc caggggtggc ccgaagagat gaaagcgtta cgtatgccag
    15121 aaaacgtcgg ctcacatctg tttatcggtg aagaccgaca tccggtctcc gcgccacaga
    15181 acgcgaatca ggtaacagaa ataaccagcg cagcccctga caagctgaca cctgttctgg
    15241 gatcagtgga taaggatacg cgtgaactca acctgttact tgtccagagt gccgatgaac
    15301 atctgcaggg tgtggtccgg ctgaacggca cattgtatcc ggcactggcc accccatcgg
    15361 ccgacaacag tcagttggtt atcaatgcgc tgaccgacaa aggtttacgc tttgcaggtt
    15421 atggtgaagc ggtaaaccac gatgctgaca gcacgaatcg ccccgcaccg gagcttatgc
    15481 agtttcatct gaaaacccgg gaagaaccgc tttttgctgc tgtctacacg ccggaaaaac
    15541 aacctgatgc gttgtaccga aacctgggct tcgagcagtc atggcagcag tggtccaaca
    15601 gccaaaagcc ggaggacagg caggaaaaaa ctctgcatca ggatctctcc cacagccccg
    15661 gccgatgagc cggtaaggaa gccatatgaa catgcataag tctctggttg ccgtgctcgg
    15721 ccttatcctg atttcatcca cggcaagtgc tgcaacctgt cgtgcgggta gcgcggcaca
    15781 ggccggtagt aatacaggat atgagcgagc acgtgcagct gcggatgcat ggtcacaacg
    15841 tgaaaacgat gtttcctcat ccctgcagtc ctgtctgtca cgaatcaaaa aaatcagcat
    15901 aaacctcccc cagttcccca gccttgacga cattctgtcg cagctggaaa accaggtctg
    15961 cgacgcggtc gtggacaagg tgaatgaaaa gctgccgggg aatattgatc cctggaagga
    16021 ctacaaccta tgaccgaaaa taccgagcca tcctcgccat cacatccggc acctgacccg
    16081 gaaatactga agagcacaat cagggctatg aagcagtcag aacagcgggc aaataatgtc
    16141 cctgcgctga taaaagcgct tttatgtacg ggcacatgtc ttcttatcag tattactggt
    16201 aacgccattc agtactggca ctcaacgaat gtggagcgcg aatatttcgc tacagacaat
    16261 ggtcgtctgg tcaggctggc accaacaaat cagccagcat ggagtcagaa tgacgcaatg
    16321 gcctttggca gccaggcact ggcgaccgca ttcaacctgg attttgtgca ctatcgttca
    16381 cagatttcct ctctgtctcc gcgtttttct gacgaaggtt ttgccggata tgtgaatgca
    16441 ttacaggcat ctaacattct ggagacgatc aagaaagaga agatgaatct gaccgccacg
    16501 acaggcgctg gcgttctggt acgacaggga caaatgtccg acggcgtatg gttctggaca
    16561 tttcagtatc ctgtccgtat gcgtctggtc ggccagacaa ccagcaagcc agaacagtct
    16621 tttgttttcg aaatcaccat ccagcgtgtt gaccccagac tgaaaccatc cggtatggaa
    16681 atccggcaga tgatctcccg taacgccgga ccgaactcct gagataagta atatgcaatc
    16741 acgttattta ctgagcacac tgctcctggt gtgcagcgcc gccacgtctg cagataacgc
    16801 aggatggcag aacgcacgca caccacaaac gacaaattct gccagcgatc acgttcagaa
    16861 cgcctcccaa aacaccggag gtgttccggc aacactggta aaaggtgagc tgcctgcccc
    16921 cgggcaggcc agcccgctgg tacaggatgc ggcaagactt gattctgagc tctccgccga
    16981 tgagattcgc tcactacgca gtctcatggc ggataacgag cgggccatca atgcgcccat
    17041 caccagtgtt gtaccccgta tcagctctct gacagttaat ctgtcccccg gtgccagcct
    17101 tcctcttgtt cgcacagcca tgaacaacct gagcgtagtg acatttaccg atatcaacgg
    17161 ttcaccatgg ccacaatccg atcctcctta taacgcggca ccaaaactct ttgatgtgca
    17221 gtacaacgag aacatggtga caatcacacc attgcggcca tgggcatccg gaaacatttc
    17281 tgtctacctg aaaggtctgt cggtaccggt catcctgaac gtaaccagtg gcgaaacaga
    17341 tacgccgtca tccagtcagg aaatggacag tcgtctggat ctgcgaatac cacggcaggg
    17401 accaaccagc ccggttgtct ccatcccgac ggataaaatc gccctgcatg acgcaacatt
    17461 acaggcattt ctggatggta ttcctccccg ggacccttca gttaagaggc tgaaattcac
    17521 aggtaatgtc ccggatacaa cgatctggca acacggcgat gaccttctgg ttcgttcccg
    17581 ggcgatattg cgcgatgaat ttgaacagac cttgtcctct gccgacggaa ctcatctctg
    17641 gaagcttccc gtcaccccac tactgacttt ctctgtgaat ggccagtccg ttcacgtgac
    17701 accagaactg gagtaatcat gagtgctgaa caggatgccg gtaaatccgg aaagaaactg
    17761 gctgccctgc ttgggctgag cagcatcatc ttatttggcg gcagctatat cgcgttttcc
    17821 aaactatcag ggagtaacag cgatatgcag tcagccgtga acataaacag tgctgcaagt
    17881 ggcggtacgc gcagcgttac cgaaacgcct cactatcgtg aactgttaag agcggataat
    17941 gaacgagggg ctgcggcggc agcccgcaat aatcagacgt tcattgccag tcttccgcag
    18001 gggcttgata ttccggacac tccggaaaag caacaacagc ctgcagcaaa accggaaaac
    18061 tatgcttatc gccaggcttc cggcacacca caggaggatc gagcagccag tgaaaagcgt
    18121 atggaacggc tgcaaaagct gattgtaaga ataaaagatc agcatcctgc cggtagtaca
    18181 ccaaccatag caacgactat gtggaataaa tctccggcag aaacaacagg gcagaatggt
    18241 acgcagcaat tcgcattaca gaatgccagc ctgtccacgc ctgtagcaga aaaagggata
    18301 caactgatac ctgccctgac ccgtataccg gcatatattg atactgcagt ggattctgat
    18361 aacccgtcat ccaaggttat cgcaaccatt cccgctggcc cctgggcagg ggcaacgctg
    18421 ttcagtccgg gagtcaaact ggtgggtaac ggcgtggaga tccatttcga ccggatgagc
    18481 tggaacggca tggaccttaa ggtcaatgca tatgctcaac gggaagacaa cctgatgtca
    18541 tccgtggcca gcaatgtgaa tacacgctgg tttaaacata tcattctgcc gtcagtgtta
    18601 ggaggtgtgg gcagtattgg cacactttat aaagatgcca acacgcaggt tattcagggt
    18661 aactatggca cggttaccgg acgcgtggga atgcccagtg gtgaagctgt tgcgggcgtt
    18721 attgccggtg gtatggcgga aagaggaagt cagatattaa cccgccaggc agaaagtgag
    18781 ccctataaac aggtagaagt gtatcagcat gaagtcgtat caattctgtt tgttgacccg
    18841 gtaatgacaa acgacgccag gtcatcttcc ctttccagtg gcatttcccc ttcagttaat
    18901 cgtacatcac aggcggaaca acgctcgcag gcacgaatgc agacagccat ggagcaaaga
    18961 aaagctgtga tgcaacgtcg gtatgatgaa caaccggaga caccataatg aaacctgaaa
    19021 cagaaataga tgaaaccggc agcttcggtg aacccgaaga gaaacccgcc cccttctgga
    19081 aacgttccgt ctgggggata tctgtagcaa catgggggct atgtgcagtc gtgttgctcg
    19141 cagccatatg gtatctgttc ctgagagcgc catcggaaac aggcatgcct ccgtttaatg
    19201 atgcggatgc tggcgtacag acctggcaaa ccacacaaga atcatcacct tccgttcagt
    19261 ccactgagac tatgactgtc cggacaggag aagatatgag ccagcttgcc cgggatgtaa
    19321 aaacagagct ggataaccgc gatgaaaaaa ttcaggcaac actgaatatg cttcacgaca
    19381 gcattaacaa gctgggcgaa gctataaaaa aggatgaaga atacgcacag gaaacacgcc
    19441 gtcagctgga tgatatccgg agccgcctga acggaataat gacacagaaa agcgtcactg
    19501 aatcctcttc cacaccgcat ccagcaaaga aaaaaacgtc gtctgtactt aacggaatga
    19561 aaatcatgtc gatggaaact ggtatggcct ggattcgctg gcagggtagt acctgggctg
    19621 tcagagaagg tcagacgctg ggcaatgtgg tgatccagcg gattgatccg actacccgga
    19681 ccatcatcac cagtgcaggg acgttacgct gatgaacatg gatgccctga ccgccattga
    19741 gaactttgcc agcagtattt tcagtgccgg aatggatttc ctgtttacct ggggagaatt
    19801 catcggggtg atcagcatga ttaccctgtt tgcccgcgcc cgttcagccg gtccggtaaa
    19861 aatgtctccc ggaaaattta ttgcggggat gttaaccagc tgcatgctcg tatcactccc
    19921 ggcaatgatt aacgccggag gcgtacaaat gggattcagg gctgactcat ttggcccgat
    19981 tgcatatgtg caacctcaga cgtttggcgc tgctgctggc gcagcaaatg cagttctgtc
    20041 gctggccaag ctggccggtg tgggatttgt catgaacggg atatccatct ggcgcaaggc
    20101 cggactcgat ggacataccg cactgagtgc cagtgaatcc gtcagtaagg gaaacgtcaa
    20161 atttattgcc ggcgtcctgc tggtctttat cgatcgtgtc ctcaatgccc tgctggcaag
    20221 cattggaatc gtcttctgat ttttttcacc ttcatataaa ccactgatat atccatttaa
    20281 ggagaaacta tgtcacgtat tcgtgtcgta gcggcccgtc tgtacgccgc atttatccac
    20341 gcacaactgt ttgtaatgtc atgccggagc aaactgacta agttcctgct gctcattccc
    20401 atgatgttgc tgcccaaagc cgttcttgcc gatggagatc ttgccgatat ggttcgcaat
    20461 gtcgaacaag gtgccaaaac agcacagtca tcatcactga ctattgcgca gttcatcggc
    20521 gtaattgcgt ttctcggggg cctgattggg ctgaaaaaag ttgggaaaca aggggggatg
    20581 ggactggctg gttgcattct atctatcgtc attggggcgc tacttgccgc cggacctgaa
    20641 atgatgagtc gcagccagaa acagctgggg atttccacaa tttccattgg ctaatcttgt
    20701 ccagggcact ggcatttatc cggtgccctt cctgttcggc aatacaagga ggcacatatg
    20761 tctgcctgga gcccggagca cataacgtta cgacgaacag aaaacggcaa tgtgattgcc
    20821 cacgacagtc gggacgatca ggaattcatt tttgcagaat gctgttccga tgaaatcgca
    20881 aacatcattc tggaagccgt gaaacgttac accagtacgg agcctgctca tgcaacacag
    20941 cattaaagac ttgtggttgt atccctttcc agaaattgat gtggttcata cacaggagcc
    21001 actgttaccg gaaccggaat taacaacgcc cggtcgatgt atctgctgcc ggcaaaacgt
    21061 caggcaccgt ttcagactgg atgactcatg gccactcaga cagcttaccg acacaatcag
    21121 tgacacacga gttcgtctta ataaggccac agaacatctg gataagttaa aaaagcgtgg
    21181 tgaaccagtc gcaaccggcg aaaaggaaaa gtacaacacg gcagtaaaag ccgctgaacg
    21241 ggcgcttgag caggcccgtc tgtctgccag acgtctttca ctccgccatg tacagaaagc
    21301 tgaaatcacc agtacagagt cgttgtcaga gaaagagcag gaactgtttc atgaagacgg
    21361 cccgccttac agcttatgtg ctttctgcca cgcctggcat tcactgaatg gttatgccgc
    21421 agcacagggc gttatggtgt ggctacccga ccttcatcca tccacggtcg tggcccttaa
    21481 ccgtcgttca ttacaggaag ttttcagtaa tgataagttc cgggtccggc ggggacggga
    21541 agcgctctct gcactgatgc aaaacagact ggcggttgag gataaattcc gcagtttccg
    21601 cccggccgat ttcgctgacg tttttcgtcg ctatccacct tccggtcgtt cgcccctgcg
    21661 tgaaaaaatg aacgggattg ccctgattct gacaccagat tcattcatca aaaaagaata
    21721 cgttgattga tttttcttca tgccactcca gcgcggggtg gcattaaaaa aatcaaactg
    21781 cacaaacctc cctcctgtaa gtccggagtt ccctcaccta tgaacataaa ccgtttaatc
    21841 ttcactatcg aagactgtct gaacacgcta tcccgtttct ctgtcgcaag cagttttgtt
    21901 gagtattgcg atttacgtac tgtaataggt ctggacagac aggatcgtga acgtcgccca
    21961 tggctgaatt cgccctatat tgcagccaca aagcgtggag aatatctctc tgtctttgaa
    22021 gtcagcggtg ctttcaggga aatggatgaa gcatccgacc agactggccc aggttcactg
    22081 gaatcgctga tcaccagcat gagtgacagc ctgaatacgg catataaaaa cagcggtcat
    22141 aaaatcagct tcgtttttga gcgtgatcct gacatgggga aagaagaaat cgaagacatg
    22201 gtcgccccac aaaagcgctc actggctaat acaggtatcc agttacagga tgtggtcgat
    22261 gagaaagtca caacactgtc cccatggctg gtccgggagc gttgctggct ggcgatctgg
    22321 agtggacccg accttatcag caacagcgat cgcaccgccc atgatgagct tgtcagaaga
    22381 ctggctgaac gggtaccgaa agcacgcttt gcccagtccc cctggcagtg gaccttatct
    22441 gcactgaaaa tccgccacga agcttttctt gataatgtgg aacaggcact gagacattca
    22501 tctgatggcc tgattctccg gctactggat atacacgagg tcgggcgtga aatacgtcgt
    22561 cagacagaac ggcacagcac accgcgtaac tggcaacccc acctgcctga agatgcccag
    22621 ccagcgggat atcgctggac tgacgatgaa tccgtactac atgctccttc attgcatttg
    22681 cagttattca acactcaggt gaccacacag ggcaacctgg ttcaggcggg cggactctgg
    22741 cacggaatgg tatcgattac tttacctccg caaaatctgc agacgtttaa cgaactggtc
    22801 cgtgccgtcc cccgcgcagt cccctggcgc atcagaatgg atctgatgcc cggcgggatg
    22861 aaggctctta atctgaagaa aacgcttctg acttacagca gtttcatttc tgccgttcgt
    22921 ccaatgtatg aatccgttat ggccctggcg gcaacggatg aaaaagaacc tgtctgcata
    22981 atgaccatca tggcctccac gtggggaaaa acacgtgaaa tatgcacacg aaaccaggcc
    23041 attctgaaat cagccattga aggctggggc gtatgtggca ccacgacaac atttggcgat
    23101 ccccgacgcg cctgggtgaa taccattctc gcggcatccg gaggctccgg cccggttccc
    23161 ctgtatcccc cgttgtcaca tgccatctcg cttttcccgc ttaacagggc aggctctgtc
    23221 tggcgaggca aagggaatct gatgttacat acagaagacg gttccgcaat tgaggttggt
    23281 ctggcgtcat cccaacaaaa taaacataca gaactcgcac caggcgatcc gggactgggt
    23341 aaatcagttc tgattaatac attgtcggaa atacagatat catctgctca gaaaaacctg
    23401 cccttcattg cctatatcga taagggatac agtgctcagg gactggttca gcttattcgc
    23461 gacagtcttc ctccggaaag aaaagacgaa gctgtcggta ttattctgag caatgatccg
    23521 gaatacaccc gtaacctttt tgacgtgatg tacggcgcta aaaagcctat tacgccggaa
    23581 aagaatttca tgtcatcggt gctgtgtgct ctctgtgtcg ataccggtac cggtcaaccc
    23641 tgtaaccccg gagacacccg acaaattatc aaccagctta tcgagctggc ttttaaagag
    23701 tacggtgaaa ataatccgcg actttatcgc gcctcaacgg aagaactggt ggattcagca
    23761 ctgcaggact cgggactgta tgaaaaacat gatgcagcct ggtgggccag atcaacttgg
    23821 tttgaagtcc gggatatgct ccacaatgcc gggtatatca tggcggctca gcgggcacat
    23881 tatcaggcaa tgccccagct tcccgaagtg tcctcgatgc tgggccatac cagtctcagg
    23941 gatgttttcg gtactgttca gcgtgatggt tcaaacgagc ttctgctgga ctacatccgc
    24001 cgcgccctgg agcagggaca taacgactac cccatgatat ccgggtatac ccggtttatg
    24061 atcaacccgg aaacccgggt catcgctgtc gatctgaata atgttgccgg tgacaaaacg
    24121 cctgcaggca ggcttaaaac cggcatcatg tacctgcttg caggtcagat cgccggtggt
    24181 gacttcaccc tgccacaata cagggatgaa gtgttaaagc agcttccacg ggaataccat
    24241 gaaatcgctc ttaaacgtat taaccaactt gatcaggagg ttaagacaaa agtttatgat
    24301 gaactgcata acgcccgggg cattgacttc atctgggaaa atctggatac ccaggagcgt
    24361 gagcaacgca agtttgctat ccgcacggtt ctgagtacgc agtatctgag ggactacccg
    24421 gaaagcgttc ttaagtcagc aaatactctc tggttactgc gctacaaacc cgaagatatc
    24481 cccgtactga gggataactt caacgttcct gaatttatgc tgaaacgctt cctgaaaatg
    24541 ccggaaggcc cggcaccaga tggtagtggt gttcctgttc tgggagtatt cagggtaaaa
    24601 agtggcacgc ttgcgagaat actcaaattt accgtcggtc cactggagct atgggcgctg
    24661 aactcatcgc caaaagacag tgccctgcgt aaaacactga caaataagtt aggttcagtt
    24721 cgagcacgta agattctggc cgaaaacttc cccagaggtt ctgcaacgtc attaattgag
    24781 caccgggcgg gccaacataa cagtgacaat gttattgaag aactggcctc ggagctcatc
    24841 cgaaaacagg gatacaatct ctgatgtgta caattcatat caccccacgc tgccggagac
    24901 ctgccagagg gcaggtattt cttaaaaaac ttgaggtaaa aggtgacagt ttctcattca
    24961 ctctgccgtc acgtgtcttc aggttacatc cggctcccaa tcccagagtg atatacgcct
    25021 cggatgccag aacacaggga aaggttacag gcatgcggtt gttgttgccg tccagcacag
    25081 atgagctgat aacaacggca atatggcagg tttctgtccc ggatgaagca ccattcgacg
    25141 ttcagcatgt catccagtgg atactccctt caccgctggc ttcgctgatg tccgatgata
    25201 cctggttatg gccatccgtt cccggaacgc cggcactgag tgccggtgac tggcctgtta
    25261 tggatactga cccttccacc ttacggcgcg tcagacgtcg ttctttcagc atcatcgata
    25321 accggaacag gaaaggttat atcaccaggc gatttcagtg tttcccactg gccccactac
    25381 ctgaaaacag aaaatacgac agccttcctg accttgtgct ccatcccgag caggagacaa
    25441 taaaatgcaa agaaaaacat tactcgcagc ccttatagcc accctgagtg gaacggcatg
    25501 ccaggctcat gcttattcag tcaccgtcgt cgccagccgg cctgttgaag agcaggtcat
    25561 ccccagaatg gaagccatca aagatgtcct tggcaacata ctgagtaccc agacagccac
    25621 gggtaccgct atcaatcaga actctgaaaa gctggcaagt gtcattgccc agaacggaca
    25681 ggcgacacgt cagcagatga ttttcagtaa tgaaactcag cgcctcgagg aggcacggaa
    25741 aagcttcacc gtgccagact ccatctgcag tgaatccgca tcagggattg caacagaaag
    25801 caaaagtgcc tcagcatccg cagcctcaaa actcagtaaa ggtggcggtg tgagcaaccg
    25861 tagcatccgc gaccgtctgg ccagcgcagc aaactctccc gtgcgggaag cttatgatgg
    25921 cgcggccata cacgccagtt actgtacaga agcagaatat gcacgttttg gaggaaccgc
    25981 cgtttgtcct tctgtgggcg aaataccagg tggagacagt caggtacgct cgatttacca
    26041 cggcgcaggt acagcggaca cacctgcagc actgacatgg gatcagaagc aaatagatgc
    26101 agcgacggct tacatgaaaa acacttcccg cccctctgcc ggtcgtgctc tgggtaaagg
    26161 agaagtgaat acccagagtg ggcgaaccta cgtcggactc cagaacgaat acaacggcat
    26221 cattgattct gcgtcgaatc cccaactgac gctgattgct gacagcacgc ctaatgaaag
    26281 cacacgtaaa gcactggctg aaacactgca gtcagactca gcggccgctt acttcgacca
    26341 ggtggcttca ccagaagcaa aagcacgggg atatatgagt acccgtgagt ttgaagcttt
    26401 tgaggctggc cggcgctacg ccaacacagc atacctggtg gatttacagg aaatgcaggg
    26461 agataacctg ctccgtgaac tggtaagaat cactgcgcaa atgaactggc aactgaacga
    26521 cctgaaagaa cagatccgcc agggtaatgt catctctgga cagcaactgg ctctgaccgc
    26581 ccgccagtat tatgaaaaac aactcggtag ccttgagaaa acaatcaatc aggcaaacgc
    26641 acgttgaaag ctgacgtcag aacaggactg aaaaaatgaa taagttaccg gaaaattcag
    26701 accagacgaa aaacgtcaaa aaatcaccat ccataacccg catagccacc cgggggatta
    26761 ttaacatttt tgttcctctg atggagactc gcctgataac gaattctgta cagcatatgc
    26821 tggaacaaca aaaacgcagg ctggacagat ttcgtcaggt aaataataaa aaggcgcaac
    26881 tccacctcac gtgggaagaa gctctgcagg ccagccatat gtcagtagac gatctggatc
    26941 gtcgtttccg ccggcgcaga acagtatggc gattctgttg ctggagtctg ctggccatcg
    27001 ctttgtttct cagtggtatg ctctttgccg cctcttcatt acctcttacg actctggtac
    27061 gcgccatcag cactcttgtg ttgatactgt caggcgtcgc tctttgtgct tccagagcac
    27121 tgattgttac ataccgcctc tggcagttgc atgaacgaaa agtcagcgaa cccgagcagg
    27181 ggactttccg tgattttctg aacgaccgca atggctggcg caatgccaca ttaattgccg
    27241 taaccagtaa acaatactga tcacgtccag atgccagtac cactggcatg tcgccatccc
    27301 ccttaattaa atcctgtgtg gcctttgccc atacgcaatt ctattccgga gttactgtga
    27361 aaattttact tcgcgcactc tgtgcaggcc ttgctatatc ctcactgccg gcaatggctt
    27421 cagtgaccta tcaggatatt gtgtctgccg caaccaatcc tgacgatctg tctcgccagg
    27481 cgctggtcac gatattcggt gatgtagtca ccaatccact gtcaaccagc gcccccactc
    27541 tgatcggaag catgttcggg gcgttcaaca gtatcatcgc cgtacttgcc gttgtctggt
    27601 ttatgttcat tggtatccgt catgttgtgc gttcggggca tcagggacag gtattcagca
    27661 ccggacgcga tatcgtcggc acactgagtg tcgttgcagg ttttctgatg atcgttccaa
    27721 ccggaaatgg ctggagtatc gctcagctca ttatgttatg gggcgcatcg ataatggggg
    27781 ttgggtccgc caatgtgatg gtgcaacttg ccgctgataa tatcgcaaat ggttattcaa
    27841 tgactgtaca gccagttcag gcatccacac gaacagcagc gcgtggcatt tttgaaatgg
    27901 aactgtgtaa gtatgcggtt aacgcaggac taaatgattt taatcagacg gtcaaatcct
    27961 cgacttcact gatgacagaa tcagctaaaa ccgccagtgg taattacact gtcacggtat
    28021 ccaatggcag cggtatctgt ggttcagcca gcctgtcagt agaaggaaac ggaacaacag
    28081 atcaatcgac gattggaaaa ttctttaatc ccttcagtaa aaacgaatac agcggtgtta
    28141 tctcagcaca acgtgcagcc atggataata tgattagcga catggacaac gctgccagtg
    28201 agtttgtgac aacatttctg gaaaaaagaa attctggcaa cggaatattg ccggatatcg
    28261 agacacggat tcagagagca gccgacgagt atgaacgcgc tgtacaaaaa tcgctaccta
    28321 ttgataacgg agaacaatcc aggaaagaag ctctcaagtc ttatctcaca acctatggat
    28381 gggtgacgct tggcgcgtgg tatcagacct ttgccactgc taaccagcgt ctggcagagc
    28441 tggctgacag agctccagct gtaacttcaa tgtcatcact tggggaagtt ggcgatactg
    28501 atttatttag tgctgttatg ggggcataca agactcaatt acagaactcc acatatactc
    28561 caacgatagg aacgataaca actcaagatg aaagtaatgc agctaatagc acagacccac
    28621 aaagtgtggt catgaaaagt atagcgccaa ccatattaaa atggacaaac caagctgcta
    28681 ctgaatggtc aggaacagga acaaccagca accaagttaa cccattaatt aagatgaaaa
    28741 acatcggcga ttatacattg ggtacaacag aaattctctg gacaggatat acaactgtca
    28801 gagttctcgc gacaatgggt gataactcta ttttcggaaa ggctgttaat ttgttctctg
    28861 gattacctaa aggatttgtt gctctgctgg atgctgcagc tcccccaata tatttcttgt
    28921 tgttcctgtt gttttgtgcg ggtttttcct tatcaattta cctaccattt ataccattca
    28981 ttttctggat gacaggcatt gggaactgga tagtaagcgt attaatagga tgcactgccg
    29041 ggcctctgtg ggcagcaaca catctaggaa catcagagga cagaggaagt cgagcagctt
    29101 acggatatat atacttaatt gacagcatga taagaccacc tctcatggta ttcggtttct
    29161 tttttgcctc tgtcgccatc attgccgtcg gcacaatatt aaactcactg tttggcgcag
    29221 cactagttaa cgtacaggta aaatcattaa cagggatatt cagccttgcc gggttcctgc
    29281 taatttacgc ccgtatgtgt acaacgtctg tagctgcaat atttgcttta caagcatatc
    29341 ttcctgacca tgttatcaat ttcttgggtg gtagagatgg tgtaaatact cttggcaacc
    29401 tcacctcatc agtaaaagat atttttgcag gttctaatag aaatattaga cacagccctg
    29461 gagttagaga ggacagacta aaaaatgtca caaaggatga taataaagat ggaataaaag
    29521 gataaattta ataaaacaac cttgccataa acactcaatc gcattaaaat atcgatgagt
    29581 gtttatggag ggtttattat gcaaatggta aaaacaatca atagatatcc tgcttggtgg
    29641 acattatatt acgcccttag atttatatat ttcataagca taccagtgtt atccattttt
    29701 attcttttcg gagtgctctc cattacatct tcaagatatg tgacgcaaga agattatatt
    29761 tatacttgcg tctgtctgtt tttactaatt gcccctgcaa tattgatgta ttcccgagca
    29821 agctcaagaa aagacaaaat aaaaaaaatt gtcgcggaga taaaaaacac cggtttttat
    29881 tcacctgaca aagaatatga gggactgtct tttactcagg gcgtctattt tggcgtggat
    29941 accaaaaaag gcactatgct gtatgctcgt gcttaccctg gaaatataat ggatattatc
    30001 ggctttgaca ttgacaactt cacacgcact gtgaccgacg ccaagacact agagatttat
    30061 acaaaataca taaatatacc aatggtctca attccgtcag gatgcatcca tcccaaaatg
    30121 atggcggata caatgcatgc aatggcagaa agaggatatg attaccctgt cgactttcct
    30181 cgcctaattc aggagaagcg taaggagtgg gagcagattg caggcatgcc tgtagccgaa
    30241 gtattttaag ataagctccc tccataacag ccggttcaaa tgagccggct tttgttcatt
    30301 acaacagata acttatgccg ttaccgataa attcgaccaa gtgctttaat cgcagcaatt
    30361 tttatttcca tatcatcact ttgactcaat ctatgtaatt cacgagtaat gttctccgcc
    30421 tgacaccggc cttcacctaa tgcatagatc gcagcaagtg taacgtcttt tgatttactc
    30481 ttcatcattt caaacaagaa agtattagct atcgcaggag gcatgctcat aatcaatcct
    30541 cttcatgaaa taaacaatgg cactcactta caaacaggag tccactatcg caagcgactc
    30601 aacttagcaa tatctctcaa tttgaatgaa aaaaacagat ggcatgtgac agggaaccgt
    30661 ccgcatgatg tccgtgacca tttaacccca taagctaacc ttgtcacaaa cgggcgcaga
    30721 acagcctgcc agacagacaa caaaacaaaa gtccaagata aaacatgaat accggtatct
    30781 caatgcgaag aaataatctc cgtagaaacc cccatgagaa aaatatcata tgggagaaaa
    30841 tcgcaattat cacagccagc gcattcatta ttttcgctgt gctctggcat taattatttt
    30901 caacacaaaa aggaaaaaat aatgaatctg gtagacgcct ttgtcaaaaa agtaatatcc
    30961 gggccatata aagaatatgg aaaatggtgg atagatgttg aatacatttc atggggcgtt
    31021 cccgggaaaa caagactgat gtttgagtca aaggagcagg cactggaagt taaagaaggg
    31081 tacaaatttt taacgtgaga tctctttttg ttaaagggca ggaagcggta actccctgcc
    31141 cttagttacg tttccaggct tatcagtcga caagttcccg gatcagttcc agaacagcaa
    31201 tcagcgcgtt tgcaacggca actactgccc tgagaacgat gattaacaga ttaatcatcc
    31261 gcttctccat cactggagga aatcaccgtt gcttactctt acattgcctg tcacaacggc
    31321 actttcagtg ttagttgaaa agagacttcc tcctgccaga gcactgaaca cagcaccacc
    31381 tgtttgttca gccaggactt aaaatcttgc atccatagtc ccgatggata tcacggcaga
    31441 agtgtcgcca gacagagcta acctgcggat tataaggatg gttgctatca aatgaacagc
    31501 tcgctattat aagaacgtta gttgaaaaga gatttcctcc ggtcagggcc acccactctg
    31561 taacggaaca gtaaaaaaac cgacctcacc agtcggtttt tttacatctg ttgtttctca
    31621 aaatccgccc gacatgcccg tcacaccgag gaaactcatg ctctgaatca gaaccaatca
    31681 tctcaacttt gccgtctcct gaaaaaactg gcacattcag ttcgataact acccataaat
    31741 acagagagaa aatccgatgt cagaaaagtg gagtaaagaa ttcgactctt gctttcaggt
    31801 ggggagcata gaagaaattg ccgatgctct cgagaaacgc agcggaagcg aggaaaacgc
    31861 gttagccatg ctggcaatga cagccttcac actcatgacc cgacgtggta tctgcgaaat
    31921 gcagaacgtg gcccccgatg gcaaaggcat tcggctggag ctaatcggct tcagggaaaa
    31981 tgaaacaata ccggatacgc ttcattgaag aagaaagggc gcaatgaggt cagccttcgc
    32041 aacaaagcgg cggcagaaaa cctgccgccg tgttgcccgt taacgtttaa cttcgtaggc
    32101 taacgttgcc acaagcactg tgtttccctg ctggagccgg agtccgcaaa gcgaatccct
    32161 caccatccag acaaaacaca gaatcgtcac acagatgacg attaacatca ttaaaaacgt
    32221 tcgctgtggc atcatagccc ctccattgcc ttacggcggt tatcgggcta ctataatgtt
    32281 gtctaggcat tacagtagag cccttggttg attgataaaa aaatctccag gggctttctg
    32341 ctgtataccg tccacaaaac gctttcggca tacagcctca agcgcccgca gctattctaa
    32401 cgactttcgt atctgcaaca atccttttat cgcacggcta attcctctgg ttagctctta
    32461 tcgcctgcaa ataaattttt aacctttccg tttgatttaa attccagaaa tggagacttt
    32521 gtcatgtctt acaatcgaca gcctgtcgca gaagatccta tgcagatatg gggcgctgtt
    32581 ggtgtccttc ttatcctgct gctattcgtt atatggttat tcttgccgga agtggtttac
    32641 gccagctgcc tgattcttca taccttatgg ggactggttg actggggacc atttcataac
    32701 tatgcagcac cacgatacaa cctgcttgcc atgaccggta acaacgccgc caacataagc
    32761 tatagccagt gggttaatgt catggagcag accataggta ttctgtggat gtacctctta
    32821 cctgtgacac tctggtgttt gtgggagtgg tatcaacatc cgggacaaag tcgtttcacc
    32881 cgcagaccag tagatattac ccgactgcca cacatctttg cttcgttgtc ccctgctatc
    32941 gcccctgtac tggcagatgg tgatccggaa aaactctttc atggtggcaa acgacctgaa
    33001 cggcgggttg ctctcacccc ggaagcattt gtcgagcagc atactctcat caccaatatg
    33061 cagctcgatg ttgcagctgc aagacgatgt ttcatggccc agttgggtaa acctttaacg
    33121 tcctggaaag acatggcacc acatgaaaaa gcgctctttg ccatatttgg tcttcagtat
    33181 ttcctggatg atcgtaaggc agcattaaag ctcatggata cactgaacct ctcatgcaga
    33241 attaaaagca aacgggacag tgggaaattc tgtacccctg tttattcact ggccaaatca
    33301 gctttccaga gagtgattaa aagcaacgga gcacagcaat ggctgaaaca acatcgctac
    33361 gtccgcagtg ggctggtctg gctgtatgca catgacctgc ggcttacgcc acccaactgg
    33421 atttggctga aaggcgtgga caggacgctg ttttatgcat tacaccgcgc caataccacg
    33481 aaaggattta ttgaaggcgc tggcgtggtg gccgttgcac gtgcagaggc cgaagccatg
    33541 cgttttggtc ttccctgccc tgaaccctgt gttgatgaag cggtggaagg cttacggcgg
    33601 gatatgctca gtctcggtct tatctgggat gaaccacaac cagatcgcga ccggaaacgc
    33661 cgcatcctga ccaactggtc cctcaccgac gacatcctgc cccgcacacc tgctacagac
    33721 aacgaattct gattatccgg agaactgaaa atgacaatcg agtactttgc aacacgcatt
    33781 gcaaaacata tttccgtgca ggcaatctgg ccggatggca ggacagaaat tattgccgtc
    33841 cttccacggg atgccctgtc agggctggta acacgaaata atcaactgat tcatatagcc
    33901 tttgacgcca tgggcacccg taacagagaa accgtcctga ttgataaaca ggagcataac
    33961 gctgaccaga tacttacagc tattgattcc tgcctgcgcc ttgaatatct gatggaacgc
    34021 cgcttcagca gaacaccttt gttccgtggc ataatcgcca gcgttgtgct gttcgtaatg
    34081 gcaacgattg gcttctctct gttccgctat gttgatcgtg tattctggga tgatacaacg
    34141 cccgaagcag tacagacagc aggtgagcca cgactgctac cccctcacct gaatcacact
    34201 gtgccattaa atgaaggcat tcaactcccg gttcccccga aaaaagacgt ccaggtcccg
    34261 gagaaaacaa tcacaagcaa aaatccggaa gccgcagctg cgcgacataa tctggctact
    34321 gttctgaagc ggaacgccga tcggggcatg tttaccatca acctgtcatc cggtcatgaa
    34381 cgaaccctgt tcgcatttct cgatcctgcc tgtcctaact gcagacttct ggagccggcc
    34441 ttaaaacgcc tggccagcga cttcaatgtc gttatctacc cggtttcagt tattggtgga
    34501 gaggaatcca ccgaccgtgt ggcaccactg ctgtgtgaaa aagatgctca gaaacgtgct
    34561 gccggctggc accgactgta ttccgcagat aacggaatga tgacgccatc agaagaaaca
    34621 acgcctgcag atgaaacatg cctgaaagca gccagagctg cgattgatgt taacaatgtc
    34681 gccttccgta agttcggatt tgccggtacc ccatgggtgc tgtccgacac cggctggcat
    34741 ctgcctacag gaattctgca ggaaactggc acgcttaatt tattcctgaa aaccaccgac
    34801 agcgagtctg gccatgagtg aacatcgcgt taaccccgag cttttacacc gtacagcctg
    34861 gggaaatcct gtctggaatg cactccagag tctcaatatc tatggctttt gcctggtggc
    34921 aagtcttgtg gccagtttta tctggccact tgcattaccg gcatgtctgt tgttcacact
    34981 gataacaatg ctggtattta gtctgcagcg atggcgctgc cctcttcgta tgcccatgac
    35041 gctggaatgt gctgacccgt cacaggacag aatgattaaa agaagtcttt tcagcttctg
    35101 gccaaccctg ttccagtatg aggtcattct ggaatcaccg gccagcggca tattctatgt
    35161 tggttatcaa cgcgtcaggg atattggcag ggagttatgg ctgagtatgg acgatctgac
    35221 ccgccatata atgtttttcg ccaccaccgg tggcggtaaa acggaaacca tctttgcctg
    35281 ggcgattaac ccactttgct gggcaagagg attcaccctt gtcgatggca aggcacagaa
    35341 tgatacggca agaacgatct ggtatctggc cagacgcttt ggacgtgaag acgatgtgga
    35401 agtcattaac ttcatgaacg gaggaaaatc ccgcagtgaa atcattctga gtggtgaaaa
    35461 aacgcgtcct cagtcaaata cctggaatcc cttctgttac agcactgagg cattcacagc
    35521 ggagacaatg cagtcaatgc tgccccagaa cgtccagggg ggcgagtggc agtcccgggc
    35581 cattgccatg aataaggcac tagtatttgg tactaaattc tggtgtgtgc gcgaaggcaa
    35641 aacaatgtcg ctgcagatgc tccgtgaaca tatgactctc gaagggatgg ccaaacttta
    35701 ctgtcgtggc ctcgatgatc agtggccgga agaagctatc gcaccgttac gtaactacct
    35761 tcaggacgtt cccggtttcg acctgtcgct ggtcaggaca ccatcagcct ggacggaaga
    35821 acccagaaaa caacatgctt atctctccgg gcagttttct gaaactttca gcacattcac
    35881 ggaagcattc ggggatattt ttgctgaaga ttccggtgat atcgatatcc gtgacagcat
    35941 tcacagcgac aggattctga tggtaatgat ccccgctctg gatacatcag cgcatacaac
    36001 gtctgctctt ggacgaatgt ttatcaccca gaaaagtatg attctcgccc gtgatttggg
    36061 ctaccgcctc gaaggaactg attccgatgc actggaagtc aaaaaatata aggggcgttt
    36121 cccttatctg tgtttccttg atgaagtggg ggcatactat acggaccgta ttgctgtgga
    36181 agccactcag gtgcgttcac tggattttgc tctgattttg atggcacagg accaggaacg
    36241 tatagaagga cagacaacag ccacgaatac tgcaacactg atgcagaaca cggggacaaa
    36301 atttgccggc aggattgtca gcgagggtag tacggccaga acgctgaaaa gcgccgccgg
    36361 tgaagaagcc agggcgagaa tgaacaacct gcagcgtcag gacggcatat ttggtgaatc
    36421 atggattgac agcccacaaa tcagcatcct gatggaaagc aagataaatg ttcaggagtt
    36481 gattgaactt catcctggcg agttcttttc tattttccgg ggagaaaccg taccatcagc
    36541 atcattcttc atccctgacg atgagaaaag ctgcagcagc gatcccgttg ttataaacag
    36601 gtatatcagc gtggatgcgc cacgcctgga ccgtctccgc cgtcttgttc cccgtactac
    36661 tcaacgtcga ataccgtcac cggaaaatgt cagtgccatc attggcgtcc tgactgcaaa
    36721 accctccaga aaaagacgaa aaattcgcac agagccgcac acgatcgtgg atacattcca
    36781 gcaacgcatt gcaggacgtc aggcggctat ggcaatgctg gaagagtacg acacagatat
    36841 caacgcacgt gaaagtgcct tgtgggaaac agcggtgaat acgcttaaaa cgaccacacg
    36901 ggaagagcga cgaattcgct atatcacgct gaacagacca gaactacctg aaacaaaaga
    36961 ggaaaatcag atatcagtga gagcagaaag agcaggtata aacctgttaa cgcttccaca
    37021 ggacaataac cacccgacag ggcgtcctgt aaacgggttt catcataaaa aaaccaaccg
    37081 tccggactgg gacggaatgt actgaacagt ggccgcgccg gattctggcg cggccttttt
    37141 tttaacctgg agaaggcggt tcccagcgtt tatcctgctc cggcggaggc agtgatgcgt
    37201 cattgttgct gttcgccgcg atataatcgc ggtagtagcg ttcacttcct tcacggtgat
    37261 gatccacgtc cggtcgcgta atcctgtagt cttcgtgacc ggtacggcct gtcacattat
    37321 gccaggcaac catttcagca aagacctggt taaattgttt gtcgtttcct tctggttcaa
    37381 aacccatgcg tggatcacgc ccgaataata ccggtgcaat caaatccagt ttatctgcca
    37441 gcgcattgcg gtcatggtga ttacggaaaa caaccctgtc accataatcc gtacagacaa
    37501 actctcccgt ccgacggtca cggaaacgaa cactcccgtt cttctgcaga cgggcctcga
    37561 gatcaccggt attaccgtat accggcgttc cgcccggttc acaaagcacg acacagcggt
    37621 ctgtcgtcgt tgtgcgtgac ctgtctttac ggcgatcgcg ataatcccag cctctcagct
    37681 gcgatacagc agccctgtct ccctgagcag cctgaatttc gacccactga cggtaagagg
    37741 gtggatacca tttcccatca gcaataagtt tctgtcgctc atccctgatg tcttctttca
    37801 gcctgatcaa tgcctgcatt cgctgaactt ctgcaatgtg ataatgcagc ttacgtaatg
    37861 ccggatcatc gtactgcgca cgaatgtgtg acttccgcag acggcatgcc tgatgaattt
    37921 cccgacaacg ttccccataa cgcagatctg gtttacgcca ttgttcacgc catgccagat
    37981 agcgggcacg caggtcagca cgggcctcag ctctggcttc acgtcgctgc cggcgcagca
    38041 ttgggtcacg tttactgctg accccgtacc tgtcactgac agccagctcc gggttatagc
    38101 gactttcagg ttgcaccctg tcaaacaaat ctgctggggc actcacaaaa ggtccggcct
    38161 gtggctctgc acgccccagt gtcagatcag gatgaatgct gctggccttc accggcgttt
    38221 gctcatgatt aaaggcatca acgacaacaa gcccgtgatg ttgtttctgt agcatcagtc
    38281 cctgttgtgc aaacagctca tgacaacgct gccaggtaaa ctcaccctgt gtgatcattt
    38341 cttcaatctg agggctacaa tggctgatgc tgtatcccca cagactttct ttgtcagaaa
    38401 acagttcgcg tttgatagcc ccctgcggcc ccgtgcctgt acgcatacgg tgccattctt
    38461 tatcggtcgc aggacagctc tccatacgac gaccacggaa gcgttctgtt ggcgtaacct
    38521 ggcggaaaat atctgttggt acaggctgcc agccaccatc gagttgattc acattgtcga
    38581 gcgtcagcag cgaccatacg ctgtcagcac gaacgggagt ctggttatga tcataaccgt
    38641 cacagataac caggtgacca tgctgaacac gcatccataa tcccgcttca gccagtgtgc
    38701 ggtgaatagc ctggcagttt tccagccgtc cttcccgtgc catttccggc agacgttctc
    38761 caagatgacg acaggcatac tgctgcagac tttctgtttc agcgattttt tccgggcgaa
    38821 catcagcggc aatgaagtcc ggattatagc gtcctggtgc ttccacctgg ctgaaaatgt
    38881 cttttggtac tggcgtgtag tcacccattt ttttcatgag tttttctgca caccaggagg
    38941 gaccaaacga gtcaagctgt acaccttccc tgttgcgatc ccagccatcc atcaccagaa
    39001 attttccgtc catctgagac aggtacagac catcttccgc aagacgacga tgcagggata
    39061 accagtcatt tacaggttca ctgcgtaaac cagcgaccgc tgtctgcgcg acatactcgc
    39121 gaaaagtttg ttttttcccg cgtgtccacg cgttctggcg atcacgttca acggcggttt
    39181 tgcgaacgat acgatttccc ggtgcatgga cccagcaacc gttatccgga gcaaaaccat
    39241 gcttcagttc aagctcacgg caggcacgac tgagtttttc ctgactccag gataaccggt
    39301 tcagataacc cgtttcaggg tgtacccggt taacagccac atggacgtgc agattatctg
    39361 tatcagtatg cacggcagag acatactgat ggtcggcaag gccgagtgat ttcagcgtat
    39421 gacgtacact gtcataaatc tgttccggac gcgggctttc atgtgactgc caggaaagga
    39481 tgtagtgaaa aacaggatca gtgtcatctt ttgcatagtg tgcctgccgg gcaatgtact
    39541 ccatatctgc agcggcagtt tcaagagaag tacagttatg aaagcaggtg acaccataaa
    39601 agttgaccca ttcgcagccg tccttcatga catcaaccag cgccacgaat gactcatttc
    39661 gaagacgtgt cgcataatca acaaggcgac tgaaacggct tcggtgcggt tgttcagcct
    39721 gcgaagaaga agataaatcc agttcctcat cggtcatgtc atcccggaca gagacgtagg
    39781 ataccaggtc ttcgaacgaa gacttgccgt cccttctctt tttcggaatg actgcattca
    39841 ccggagaatc ctcaaccttc cattaactgt ttacgcaatg tatttgtaag ttcggttatg
    39901 gcaacaagca cttctgcgta ttccttgtca ccggttcttt ttccctgaac aaacagatgc
    39961 ttctgcatcc ttcccagtct cattaactcc ttcagaaaag catcatctgt acgggagtta
    40021 attcgcttat tcagtgctgc atttcggatg taagcagata cagtaagtcc ggaatcttca
    40081 gcttttttcc tgatggtttc atcctcaaca ggagagaatc tgagtgtcct gacaactgtc
    40141 ttttgccgta cctcactttt ctttcttacc gcactatcac tcacttcagg ctccttacgg
    40201 ggtgtcgggg cgaagccctg accagatggc aattgtaata gcgtcgcgtg tgacggtatt
    40261 acaattgcac atcctgtccc gtttttcagg ccattatagc ctgttatttt tgtgcatcat
    40321 agtcttatgc agacggcaga ggcagggcgc agccctgcca ggagtcagtt tttgatgata
    40381 aaatcagact tcaacatgaa acggagagaa agtgccaatg gcttctaaaa aattttactc
    40441 agatgatgac atccagctgg caaaagctgc tttatcggaa ttgccagatc tcaccgctca
    40501 acggaaaaca ttgcgtgatt ttcttgatgc cattcgtgat gacattattg ttctggtaag
    40561 gacaaagggt tatacactgg cagatgttcg tgacacgctc aaaaatgcag ggtatgaagt
    40621 aggagagaag gcacttcgtg acattattcg ggaagcagaa tcgaaaaagc catcccgccg
    40681 ttcttcctct aaaacagcat ctaaaaagac agattcagcc aggaaagatg gcatcgatat
    40741 gaacaacaat tgatgtgaca atgtcacatc atgttctgat attatgatga ttacagaaaa
    40801 aaagccccgg cataaatacc agggcttttc tgtttaatca gactcgtggc ggcaatgaac
    40861 caggataatg ccgtgcaata atatcattca gtttttcaac aaggtcagga cgcttaaaga
    40921 caatgtgtac ggtccctttc tggtagccac gcaccgtaaa atattcccct tctaacagct
    40981 taccgacatt gtcgccgttc ccgataaatt caccgtactg cgccccgatg ctgctccgga
    41041 agtcaggaac gttgcgcccc tccagcagat aaaacggacg taccaggtca tcctgtgcct
    41101 cccgtccgct gcagtccagc gtaacacgcc cgttactcca gcggtacagc aggttttcaa
    41161 taatgattct cttgcccaga cggcagggat tattggttct gtaatcccat gacaatttcc
    41221 ggtagacatc aatcagtccc tgttcaaatg tctgcatctt actggcattc agatggcgaa
    41281 aagttgccag tacattgtcc agggtgattt caggacaggt gtcgctcatc agctggctgt
    41341 tccactcatc acgctgacat gaactcatga aggtgtacat ccccgtatcc gtcatcaggc
    41401 ggtcccagat tttccggtcg gcaagacggg tgatattcct tttcgcttct tccggtttat
    41461 ccatgcaccg aacggagtcg gtcacgcagt cttcaaaacc gtaaagaaaa atatcacctg
    41521 acgcatcaag cagaatcctg cgcgctgtcg ccaggcattc catcgcctgc atgaacagtg
    41581 caataccttc gttacgctgc gcgatgatgc ggtcgatggc cacagacgga ataacatctg
    41641 tcgtgccggc agtattcagg tcagcagtgg gtaaagtctg attcatcaca ttgtctcctc
    41701 atcgtcacga ctgccgggtc agccaggtca tttcccggag gagcgaagct ccttctgccg
    41761 tccgggtctt cggggatttc cccgcagtgg acctttccga cgggggacgc tcaccacgtc
    41821 acggggcagt tgtccggggg cagacgcaaa agtttttgac gcctgcggca aaaagttttg
    41881 tggctggtac atctctcccc tggacgactg aaacgggatg tggtcgtgta tgtacccccg
    41941 gaaagtccat ctgcggagaa caggccgcag tgcggcggca gacaggcaga gacagctgcg
    42001 ccatcaggcg cagtggcctt acacaccgta aggtgtgcag ggagcgccgc atccggcgcg
    42061 actgaagaaa aacgacggcg caacaatggc atgtggtggt cactggcaca ccactcccgc
    42121 acaggtgaaa acagagaaaa cagcacgaca cctacaatca aaaaaagtga aattccttca
    42181 ctttttcaca cttatttttc gcgctgattt ggtggttatg gtgataaaat aaagtgaagt
    42241 tttttcactt tttcaaaggt gttttatgac cggctgggaa ctgcgaatct ggcgaaaaag
    42301 catgctctgg tcacgcgaaa aagcggccag agaatttggc gtgacacaga gaacatggca
    42361 cgcctgggaa aatgcggaac aggtggatgt caccgtctgg aggaccaccc aggcactttc
    42421 agtccgtgac ctgctgcctc acatgcaggg gatgcgcaag gcagatatca tcaggcgtct
    42481 ggaaaatgaa ctgggggaaa cggcagagga tgtgtgagcg tggttcacag cctggcagaa
    42541 tgccaggcta ctgctcatca ggaaacagtt ttttcagttc ttcaccggaa agtcccgtgg
    42601 cctcctgaat ccgttccggc gtccagccca tatcccgaag ccggcaggct atttcccgcg
    42661 ccacttcaag agcacctttt tcaaaacctt tctgtataag acgttctgca atcgtcatac
    42721 tgttttctct gctcctgctg gcaaaaggcc cggcattttg ccgggccttt tggctctcat
    42781 cagtgagtga tgttcttcag ttcagcttca gaaagtccgg tcagctgaca gatttgtgct
    42841 gccggcattc ccgcgttccg gagctcccgg gcgatttccc gtgctttttg aagcgccccc
    42901 tgctgaactc cctgctggat accctgctgg ataccctgct ggataccttg ctggatacct
    42961 tcctgcttaa gacgttctgc cagtgtcata atgctgtcct cgtgctgcgg cagtcgctgc
    43021 gccagcgtct gcaaaaattc aaccgttaca tgttcgctgc cattaagcag atagttgaaa
    43081 agaactgtaa tctgctcttc atcctggatg tcctgcagtg ctctgaccag gttgtccaga
    43141 cacatcagca tatcccgctg acggatatgc ttcatcagca gcgtcagtgc ggccatccgg
    43201 cgatggttca tgatttcatt gtcatcaacg acagtgatat caaccagcgg aaacggcttt
    43261 gtgtatattt tagccgcaag cgccggattc tcaaaacagt ccagccagtt catgctgtac
    43321 gggtacgggc tgcgctcacc gtgatagaac agcaccggaa tgaccagtgg caacgttttg
    43381 tgctgctcca gatgtctctg catcgccgcc acactgtagc gtaacatgcg aaaaggcatg
    43441 tacagatttt cggtgctctg gtgctcgatg agcgtataaa cataaccatc ttcacccccg
    43501 gtggttttca cgctccagag gatatcgctg gcgtactgat gcaggtccgg ctcaacaaag
    43561 gtggcgggtt ccagcttcag cgtggacagg tcgcacaact gccggtactc cgcaggaaga
    43621 tgcagttcca gaaaatctct ggcgacgtcg ggattcgcca ggaacgaccg gaacgcggca
    43681 tcatgcggcg tgggcgttgt ggtgtttttc ttcttactca cggttcctcc tggtgagatg
    43741 tctgaacgta tcctgccgcc tgcacaggca gacggcaagg gttaatcagt ggcagacccg
    43801 ctcaatttca agcgcgcgga gcaggtggcc ccattcgctg actttcatag cggggatacg
    43861 cacccgcttc tgctcactgc agcgtctggc gatatggccg ctcatcgcca ggacgtccgg
    43921 gttaatctca tcctgtgcca gctcctgacg tctgaagcgg gtatggcggt gcaggggttt
    43981 attctcccgc aggcactctt cacgcagacg ctttacatac tcggcggttg cgaagataat
    44041 tgatggtctc acaatattac tccttatgtt gttgatgact gccgctcttc ctggcgggga
    44101 agtaacggcg gagagaggca gggtttatcg ccctgccttt ttttcatttc ggacagcgac
    44161 gcagtgccag cgcgagcgct ttcatcttcc agccttccgg cgtgcggtac atcaccttcc
    44221 agtagccgtc gatgaacaca taatggaagc gcacctcttc agtgctttca gtggcaatag
    44281 cctccctgaa agaacggtac tcacgcgcct cactgtccat ccggaaacca ctgtcacgtc
    44341 cgtaataagc cgtcacaccg tccaccgggt tctgaagcgt atgcccggcg ggtttgtcac
    44401 aacggggacc gaggcaacgg atatcaccgt gttcaaccag ggcttcggcc tgctgaccgg
    44461 tggcatagtg agtatgcagg atgcgaccgt tccaggtcgg ctccccgtgc aggtggcagt
    44521 aaacggtacg gaacaccccg tcgcggcaaa aaacggaaat cgtgctgctg actgtcatga
    44581 aaacatctcc ttctgctgaa tcaggtcgcc gggccgaccg gttgctcccc ggaggggcga
    44641 aagccccttc tgccgtccac ttcttcgggg tttctccccg cagtggacct ttccgatggg
    44701 gcgtccgtca ccacgtcacg gggcagctgt ccgggggcgg acgcaaaatt ttttgccggg
    44761 tttatggcta aaaattttgt ggacggtata tcttcccctg gactgctgaa acgggatgtg
    44821 gttactccag cccccggaaa gtccacagcg ggaaacaggc cgcaggggtg gcggcggaag
    44881 ggttgactgc gccatcaggc gcagttgcct tacacaccgt aaggtgtgca gggagcgccg
    44941 tttccggcgc gacaggggcg gcgcgaccgg aacggtcgct gcaagcctgg tttttcagac
    45001 atcggaccgt agcggcagcc cgaaaccgcg ccagcgggtt cggcggagac tggcgggtgc
    45061 gccagcagct gcctgtcgga gtcgggcctg acgaccggat gccgccaggc agccgggagc
    45121 cgaaggtgtc cccgcctccg gaaatcagaa caacagaaac gacataacag aaaaatgcct
    45181 cacaccatga cgggcaggaa gggcagcgcc gcaggcgcgg ccctcctgca tttcaggcag
    45241 gcgtaagcct gccagggttt tccggcggcc tggcaggcca tacccccgcc ggagcggggg
    45301 agtaaatcac cgacggaaca catgaccgtc aataaaggtg aaggagtcca ggaataaatc
    45361 ccgcgcatac gcctcatagt caaaatagag cgccaccgtc tccggcacgt cagccagcag
    45421 gccggtatca ctggcgaact caaccgcaaa agcctcctca ctgtcagcct cgccccacca
    45481 ggcatcgcgg aaggtgtcaa aatccgtctc accggtatca tccacccaga gacgataagc
    45541 ctcttcgcag ccttcatccc gcgcctggcg gaagccttca acataggccc agttgatatg
    45601 gcattctgag gccatattcc ccgggaatcc ctcataatcc tgaaacatca gttcaggatc
    45661 ggcttcatcc tggtgaagag agcggcaggc ggcgaaaaag tcgcgttcat catcaaacgt
    45721 ggccaggtca aaccagcggc cagcgatgct tccacagttg tatttgtgcc aggttccaac
    45781 gtatacagca ggtgcaacaa cagacattcc cgaatcctcc atagtcacat gttctgcgac
    45841 accgggcggt gccgtgcctc cataaaagcc gggcgataag gcggggatgc acagggcgca
    45901 agggaagccg cagcctgacg cacacggctg gcgggagttg gagggtgaga ccgcgaaatc
    45961 tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg gtgaccgcag
    46021 cggcgaagcg tacccttgca tccctgccag cccggctaag atggaggcac ggcacagccc
    46081 gcacacgacg ctgaggccac agagctgaaa acccacaagg ccatgccccg catggccggg
    46141 taaaagcgga gccgccggcg acttccttat tccggcgagt gcagaggaca cgccgcatga
    46201 cctccagacc acccctgata tgaaacgatc atgatcggcg tgtccctgcg taaaatatac
    46261 atgtctggtt caccggagaa tatctgtgat atccacaagt tttctgtcgc acagccagtc
    46321 ctgcgcttca cgcaccacgc tactgtcggg tttcaccgct ttcagcgatg atgccagtcg
    46381 cttgatcaac ataaccagtt cccgtgtctg tatgtccggc attgtctccg gcggagcaga
    46441 gggactctca tttccgacgc ccatcccatg cgcttccagt gtggcacgga caaaccaggc
    46501 ggcatcgacc agttgctggc gaatccaggt cagatcggca tttctgtcac gggctgccat
    46561 tcgctcaccc gacaacgcct tgctgatatg gctgcgcacc agattatcca ggagttcctg
    46621 cgcttcttcc ggtgaaaagc cacctggtat ctctgtgcgc aacagtcctt tcctgtacag
    46681 tagctgcagc agatcaatct gcgaccgcac tgcaactatc agtacataac ggacaatatt
    46741 cgcactggcg gtggtttctg tatcattcag cagttcctcc ggatcattac caccggtatt
    46801 gcagagcaac ggcaacagag gtcgcatcca cacgggaacc tgatattgtt cgtctgattt
    46861 catcatattg cgttctccag cttgttcggg atcatccact gctcaagtgc acgtacctgt
    46921 accggtgcac cacgaccggc ctcaccggct tcaaaaatca gccgccagag tggttcctcc
    46981 tgccagaaaa accggtcaaa tgatgacagt gatggaaaac gcccgcacca cccccagaaa
    47041 atcgtttcca gatcatgctc ctcaacaaca ccagaccagc gggacaacca gcttccgacc
    47101 gtggcgggag tatgaaacgc aaggtcaatc tgcgcctgag caaaacatgt ctggtattcc
    47161 agttcccgtc gttctgcctc acgttcccgc tggcggctgt atttctgctc tgtcagcgtg
    47221 gttttctgac gacgccgctc accttcacga aaaggaacgg acgggaacag acgatgcccc
    47281 gcatcggatg gcaggggaag aacacagact tcaccaaaac tttcaatcag tttatccacg
    47341 gcagccagcc acaggagacg gttgccatga aattcctcct tcgtcagcgc aggagcaaaa
    47401 aagcggagat cggtcagcga aatccggcga ctgccattca ggcagcgaaa gaaagcgctg
    47461 gcataaggcc atgctgaaag tgtgcgtccc tgacgacgac gttgttcggt gacagccaca
    47521 gcctgcatgg cggcctgttg ctctgcgctg agggggatca gtgcccgtga acgtgcgctc
    47581 atgcctgacc tccttctgcc atcaggatat ggatgatgtc gttaacactg tacccctgcg
    47641 catcaagtgt cgcggcaagc gccagcgtgt gactgatagc gacaggcgtg aagatatgtt
    47701 gcacctgaac gacggcgaaa ggctgtccac cacaattcac cagaaccagc atccagagct
    47761 gagagacgtc tcccggcgaa cataatgcca ggtggaaacg ttcgtgggct ggcgtgaaac
    47821 gaacctggac ggggaaaaag cccccgaact cactgccata ctcgccattc atcatccagt
    47881 tagccggtgc gtccacttca cgcatcacct cgtgagtgag attacggcgc atatcgcttc
    47941 ccgcagcacg gatatcttca tattcctgtg cgttcatgga ctgcagggca ttcagggtca
    48001 gttcagtttt catcacggta tctcccttgc tgccgccccg tctccggcgg ggcggtgtgg
    48061 ttgttcatca ggcggcatca gccaggttat cggtgcgttc agtggcatct gtctgtggat
    48121 gtggtgcaca catccagcct ggaacccagc ggttgtcttt catatggtgc tctgcatgtt
    48181 cagccgcatc acctttcttc atcttctccg cgtcccgtgc ggcaccggac agtccggcct
    48241 cattcagggc tgcgataatc tgcggctttt tcaggtgtcc gaagaagttt gcttttgtcg
    48301 gctgccacca gtcgcgcatg tggaatccga tagcgctttc cagcgagtca agcggactgc
    48361 gtgacgtgtg accacactca cgggtctgga caccgttaag gctgcatgcg gtgcagaaac
    48421 tgagcaggga taacagcacc tcctgactga gggacaggaa tgtcgtcatg tcccgtgacc
    48481 atccctccgg cagcagggct gcaagacggg ctttttctgc catcagcgcc atgaatgcgg
    48541 caccttcctt ccccgatggc gcatcgctgg tcagcgaata atgttcacat tccaggctga
    48601 tttgtgctgg tttactgtac gctccgctgc caaacacatt caggcagagc gtccatgcca
    48661 gcagtgccag agatttgtcc ggttgctgca tgagtgccgc ctggactgcc agcgtgcgtt
    48721 cggaagacat tttcgtcagc agtggcagac tgatttcctc cactgacgct ttcttctgca
    48781 tctgttccgt gcggtcagcg tcgtcagtcg cgtcatcttc actgcgcaac tgcacaccac
    48841 gctggacaca tacgttgcca taacgccagg acaccaccac accgcttccg gcacgtatct
    48901 ccggcgtcca cgctctgacc ttcgccatgc agcgcatcag cttcatttct gcttccagca
    48961 ggtcggattc ctcacactgg ttttccagcg cgtcgtaacg cgtcatcagt tcgttcaggc
    49021 gttcgtcttc cgcctccgtc agcaccgctt ccggctccgg cagacagcgg tatgttccgg
    49081 catcctcacg gcactcaccg acaggctcca tgcgcccggc gcaccattcc cagccttcgg
    49141 cttcccgaag gaactctgca acagcctgga gtttttccag cagggcggca tcgagcgcca
    49201 cgcggtcgac ataaccgtcc ccgtcatcgc tgaacaaatc ggtgcgcaat tcgtctggcg
    49261 agaaggcatc agcccccaca aagcggaatt tagtgttgtc cttcactgcc acttcacttt
    49321 ccgtaatcag gttgcggata acccgcacat ccggtttacc gccccatcct gactggcagg
    49381 ccgcttcaaa cacctgcacc tgacgcgcgg tgtcgctctc cagcgccagc gcctgacagt
    49441 gctcggtggt gatgcggtcc tctgccagcg catcgaggat gacaggcgca aggtctgcca
    49501 gtttcagcat tcgctgaacg tggcggggtg aatagcccag caggtcgcca gtctgtgcag
    49561 gtgttttgcc ttcctgcgcc aatgcgcgga atccggcaat ctgttcggca gggtgcatat
    49621 cccgacgatg accgttctcg gtcatcgatg cggcagtcgc cagttcctgc gggatgacct
    49681 tcacacggac aggccagtca gccggaagga tgttgcgctc tgccagcatg ttgagtgcag
    49741 ccagtcggcg accacctgcg gcgacaccgt aacggtcacc aggcagggta tgcacgacca
    49801 gattctgcaa caggccaacg cccttaatgg acccagccag ttcgctgacg gactccacag
    49861 aatacggcac cgtgcgcaca ttcagcggtg atttaatcag tgaggccagc ggcacgctca
    49921 cttcctccgt ctgcgccagc agggcagaca gggcagtttc ctgcgttttt gcagatttac
    49981 ggcttgcttt gcgggtggtt tctggttcac acttcgttac aggcataatc atccccttat
    50041 aattgcttat gtcctgccgg acgttcgcgc gtccggcacc tctcttactt aagaatcccg
    50101 aaatcacgga tggcatggtt catccagtac ccggcaccgt cctcaaaatt ccaggtacgc
    50161 cagaccattg ttccgttatt acggacaacc aggcgaaaat gttctccgtg gtcatcctcg
    50221 ataaagacgt tccggtactg tgcggcaacg gcttccgcct gttcgcgggt aaaggagccg
    50281 tccggcagtc cctgaagtat tctgaaatat tcgctcatcc tcatatctcc ggtgatgccc
    50341 cgcacagggc ggggcggttg tcacagtcag cccgatcaga acgggatgtc gtctgaaaac
    50401 ccgtaatcct ccccctccgg cgtttgcggc tgaggctcgg gctgcgcggc cttacgtcca
    50461 cggcctttcg ttttcgcgcc accttttttc gtcgcgtcag cactctgtgg ctgcccattc
    50521 tgctgaggct tcggttgcgc ctgagcgttc tgctgtggtg cactccccag catctgcatg
    50581 gtgcccgtgg tcttaacaag aatttcagtg atgtagcggg tgatgccgtt gtcgtcccag
    50641 ctacgggtac ggagttgccc ctcgatgtag acctgcgcgc ccttgcgcag atattcacct
    50701 gccacttccg cgagcttgcc gaacagcaca acacggtgcc attccgtctg ctcccgcatc
    50761 tcccccgtct gtttgtcgcg ccagctttct gacgtggcca cctgcaggtt tgccactgcg
    50821 ccaccgttgg ggatgtaacg gacctccgga tcattgccca gacgcccgac gaggatgacc
    50881 ttgttgatac cacgtgcact cataagaacc tcctgactga aagatgtgag ataaaccgga
    50941 taccggagac gggacgttca tgtgcccctt cagaggagcg aaagctcctc ctgccgtcca
    51001 cttcacaggg gtttctcccc gcagtggacc tttccgatgg gggcccgttt accgcctcac
    51061 gggacagctg tccgggggcg gacgcaaaat tttttgccgg gttcatggct aaaaattttg
    51121 tggccggtgc atctcaccct ggacagatgg aacgggaggg ggttacgtct gccccccgga
    51181 aagtccacag cgggaaacag gccgcagagg tggcggcagg agggaagact gctgcgccat
    51241 caggcgcagt ttcctcacac accgtcaggt gtgcagggag cgcaggtcac ggcgcgacag
    51301 aggcggcgcg accggaacgg tcgctgcaag cccggttttt cacgcatcgg accgtagcga
    51361 cagcccgaaa ccgcgccagc gggttcggcg gagactggcg ggtgcgcaag cagctgcctg
    51421 tcgtagtcgg gcctgacgac cggatgccgc caggcagccg ggagccgaag tgggcgcaat
    51481 aaaagacagc cagaaacagt gacagtgtca cggaatatcg cacctgttcc tggcatacag
    51541 ccatacacta tcttctgacc ggaaagccgg gccggcagcg ccgcaggcgc tgcaccccgc
    51601 atttccggca ggcgtcagcc tgccaggggt tttcgcgcgg gctgacaggc ggcctccacc
    51661 gcctgccctg tatcacctgt ttctgctgcc ctgaaggata tagtgaccac cgaggaaaac
    51721 cgcctgctga ttcaccacaa aatcgcagat ttcatcaaca atctgcgcag tggtgagcgg
    51781 tttctgcccc atctcacgac gccgcgcatt aatcacggct ttgtaattgc gcagaatttc
    51841 gcgggtttct gtgctgatat tcatgacatg tgctccccgt ccagcgcctg taacacaacg
    51901 tccatcgctt cgcgttcttc cctgacgaat gcgtcaccgt cgcccccgtt ctcactcccg
    51961 acggcataca ggcgcttcat ttcactgcct gccagcgcca ccactttttc cagcatcacc
    52021 gccgggactg tcacctgacg gctgacgcgc ttttcttcca tcagttcacg aacaacgtcg
    52081 tgaacaacgc tgacatccgc accatgctca aaggcatcgt ccagccgctg catgacggtt
    52141 tccagctcat cgtctgacag gttccagtcc tgggctatgt gacgcacatc cccacgggtc
    52201 agcaccacgg caaaaacgca ttcactgtcc ggcacgtctg tatccagcac ttcgcggata
    52261 atttctttaa cagtacagta cattttctcg tctcctttac aggggcggca ttgccgcccc
    52321 gtccgttaat cagtccagag ccgcgtaaat cgcgttgcat tcagggtgaa attcgatgtg
    52381 acgccacaac tgcgcatcgc gcatccggta aagctgggtc agtcctgcat caccgctgtc
    52441 gtggtacagc cacaactggc ggttaatcac aagggaagtc agaatgatgc cgcaggcatc
    52501 agcactcacg gtacggtcaa accagtttgc accgttcact atgtggaaac ggtcgccatc
    52561 cggcatcatg tagccgccac cgtccggcag gcggatgaag tgccagaatc cgccttcata
    52621 ttccggcatc atttttttcg ccagcgcgta cacagacgct tcgacataca gaaaatcctg
    52681 gccaaacagg taaggcagaa aaaacagacg gtcgcactct tcaacattca gagtgacagg
    52741 tttcgcatat tgcatgatgt attcctccgt tgttcacata ttccgcgaca ccgggcggtg
    52801 ccgtgcctcc ataaaagccg ggcgataagg cggggatgca cagggcgcaa gggaagccgc
    52861 agcctgacgc acacggctgg cgggagttgg agggtgagac cgcgaaacct gagcgggtga
    52921 acgaccggaa aatcacggca gacgggaagg agggaccggg tgaccgcagc ggcgaagcgt
    52981 acccttgcat ccctgccagc ccggctaaga tggaggcacg gcacagcccg cacacgacac
    53041 ggtggccaca taactgaaaa cccacaaggc catgccccgc atggccgggt aaagcggagc
    53101 cggcggcgac ttcctttgtg ctctgtccgg ggaccggcag gaaaaaggcg gggatttacc
    53161 ccgccagtgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggt
    53221 ccgggcttcc cggaccacct cccgcagaac accacggtca aaccagcggc ggggcggttc
    53281 atcgggtata cgcagcacat cgcaggccag cccacaggac aggatatgct caatacaatc
    53341 gctgaccagg tacatttcca ccgacgaaaa acgtgctccc cggttcacga tggcatcgag
    53401 caccacgggc aggttttcgt catcttcatc gagccagcag gggtgatcgc tttccttcac
    53461 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccggtgg tggtcagcgc
    53521 cgcacggata cgccgcgcat cttctgccac ctggtaacag atgcttcgta ccgcatcgtg
    53581 caccgcatca agaacagccc tgatttcgtc ttcggtcagc cattctccgg catacgggaa
    53641 agacacctgc gttccgtctg ccggaagtac cggcagcgac tgccactgac tgccggacag
    53701 taccagttcc gccccggttc cggtcagtct ctgaatggcg gaggccagga gcggcggcag
    53761 tacgccgccg tgacggttga taagtaacgt cgccagccgg ggcgcatcca gccgcacgta
    53821 aatgctgatc cagcccggat agttcggatt tcgctggtca acatccgtct cttccggcca
    53881 gccctgcgcc agcagcaggt tacgggcgtg acgacaaacc agtgcattca gcgttttgtt
    53941 catgattaag cgttctccct gtatcgccgc ccgcagggca ggcggcgata acatcagttg
    54001 ttttcgtgtg tgttgttttt cagcagggtg cgggcctgat taatcactgc ctgacggcgg
    54061 gtaacacatt ccgggacggc aaggccgtta tcacgggcgg cccattcacg tttccacagt
    54121 gcctgttcag cggtctgaat cagggacgcc agcagttcag cagggacggt cacctggcgg
    54181 ttttctctca cgttgctgat aatctccatc gccacgccgg aagaaatacc ggactcaatc
    54241 cgctggtctt ccgggatgtc ctccaggcgc gccaggacgg tcctgatttc atgatcggac
    54301 agggaaatat ccattccgtc agcgagggcc tgaatctctt caggggacca gacaaccagc
    54361 atcagtggcg tgtctgccgg atactttgct gccagctccc ggcagagtat ttcgcatgtt
    54421 ccgtacatac tctctcccct caatctttga agcaggaatc cagttccaga atttcggtat
    54481 atgcctccat caggaggcgg tttgcgcgaa acaacgtctg acgcaccaga cgttccggca
    54541 gggtgtcagc attaaagctg taccgccgct ccatctcatt catggttgta cgcaggtcgc
    54601 acatggcatt ggccacctcc gccacttcgg cgcgaagttt ttcatgtccg gcatagttca
    54661 tttacgcagc ctccggcata aaccagtcat catttgcggc cccctgctgc atggcccgct
    54721 gtacggcagc cagacgttgc tgtccggcac ggtgatactg ctcaagcagc tcgataccga
    54781 tataccggcg tccggactga agggcagcca cgcaggttga gccgctgcct gcaaacgggt
    54841 ccagcacaat cgcgttcgga tgtgtgaagc tctcaatcag tggttgcagg ctggtaacag
    54901 gcttttccgt cgggtgatga cgattgcctg aatatttcca gcccagcacg tcgggtaatg
    54961 gcttctgagg cagcgccgga cggccttttg ccagaacgta ggcgcattcg tggcgataac
    55021 ccacatatgc ggccttcgat gtgtagtttt tggtgaagac caggtgccca accacactga
    55081 atcccgcatt tttccaggcg gccataaagc gatcgacgcg gttccagccg tagaagctga
    55141 ccatcagcgc atcttttttg agtacgcgat acatttcatt acaggctggt tgcagccatt
    55201 catcggtttt atcgccggcg atggtacgcc cctgtcggtc acgaaaaccg acgaggtatg
    55261 gcgggtcagt cagtatgaaa tcaatgccgt tatcaggaat acgcgccata acatcaatgc
    55321 agttaccgag gacaaaacgg gacataacaa cactccttct ttctcagagg gcaccgggag
    55381 ggcacccctg tggtgcccgt tccccgctgc ctgtgggctg ttcaccgcgt ccggtaacgg
    55441 gtcaggccga ccgtggaata tcgcagccgt cgtctttttg acggcgagaa tatgccgcga
    55501 aagcggcttg aagggcgttc atcagaaaag ggcgacaggc tggcgtacac tgtcaccgac
    55561 gccagccggg agcggtaaaa gcgcccgtcc cgtgcccgcc ggacgcggga cactgaccac
    55621 aggcagcgtt tatgccgcgc cgcatcagcc ggcgcagtaa acagaggagc acggcgacat
    55681 cctttgtcgg aaaagaaacg ggcggttgcc cgcccgtctc cgtcatactt catcccccgt
    55741 aatgcgccac cttgtcgact atccacgcag gtccggtgac ttccggcagc tcatcgtcat
    55801 ctgtcgggga agaccattcc agttcccccc agagcacatc aacgagctct ccgcgctcat
    55861 acaactgcca gccacagaaa ttgcagccct gttcggcgta ccagtgctcc agcgtgcagc
    55921 cgaaacggcg gcttaatgct gcaacaacgt cgctttccgg ctgacaccac ggcgtgtcga
    55981 aatccacctg aatgcagttg tctccctgac tactgatatt caggtcatac ccacaaggcc
    56041 acttcacgcc ataccgttct gtataccagt gatatgcaga aggaacaccg ttcagaacac
    56101 caccattaaa gccattcact tcaacatcca ggcgggtcgg cagaaccgtc agcaggtcaa
    56161 acggcatggt tttttccggc actacgttgt cacacagacg gttccaccac accgatacat
    56221 cctcgctacc ccagatgtca caccagtcac ctctttttgc ggtgaaatgc acagtgatgg
    56281 tttccctgac atcatccggt aatccttccc agcgacgctg tccagtaccg ctctgcagcc
    56341 agagttcatg cagcatcagg cagttttgct catccagcag tacaccgttc tgcaggtgtg
    56401 tcagccagcg ggtgaacgcg atattttccg tcgatacagc accacgtccg gcagccgtca
    56461 ctccggggca ctgttcagag cggatatttt cagtgatctg cagaagtccg gcacttccgg
    56521 ccagaaacag ctgaatacct tcatttgtgg cgcggcgata aagcggtgta actgcaccgc
    56581 tggccagtcg tttaatctca gcgatctgtg ccggttcacc agaaaaatac atacgattcg
    56641 agcaccagtt tggcatggga attctcctct cacatgcggc ctccatcagg ccgtgccgca
    56701 cctgttaccg ggcgaccgca tacgaatgca ggtgcggccc cgaagccgca gcgcgtggag
    56761 gaggaacgac gaaacgtgac cgggtgagga ggccggcgac agcgacacac ttgcatcgga
    56821 tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag gtattttgtc cacagaaccg
    56881 tgcacaaaat gctgtggata agctggacac agcagcccac agcaggcata cagccacaca
    56941 ctcagagaat tgagcgtttt ttagtcacaa caatgacatt tgactgtgtg cggattcagt
    57001 tattagaata ctgtatatac atacagcaaa agaaaaggga gatgagaaca tgatccgcat
    57061 tgaaattctt ttcgaccgcc agagcacaaa aaacctcaaa tcaggcacac ttcaggcgct
    57121 acagaatgaa atcgaacaac gtctgaaacc acactacccg gaaatctggc tgcgtataga
    57181 ccagggcagc gccccgtctg tatctgtcac aggagcccgc aacgacaagg ataaagaacg
    57241 tatcctgtct ctgctggaag aaatctggca ggacgacagc tggctgcctg cagcatgagt
    57301 accgtttatc accgtccggc tgacccttca ggcgatgaca gttacgtgcg accgttgttt
    57361 gccgatcgtt gccaggccgg ttttccttcg cctgccactg attatgctga gcaggaactg
    57421 gatctgaaca gctattgcat cagcagacct gcagccacct tctttctgcg cgccagcggt
    57481 gaatcgatga accaggctgg cgtgcagaat ggtgatctgc tggtagtgga cagggccgaa
    57541 aaaccacaac acggggacat cgttatcgct gagatcgacg gtgagttcac cgtcaaacga
    57601 ctgctgttgc gcccacgccc ggcactggag ccggtttcag acagcccgga gttccgcaca
    57661 ctgtatccgg aaaacatctg tatttttggt gttgtcactc acgtgataca caggacgcgg
    57721 gagttacgct gatgtttgca ctggctgata tcaacagttt ctacgcctca tgtgaaaaag
    57781 ttttccgccc ggaccttcgc aacgaaccgg tcatcgtact cagcaataac gatggctgtg
    57841 tgatcgcgcg cagcccggag gcaaaagccc ttggcatcag aatggggcag ccctggtttc
    57901 aggtgagaca aatgcgcctg gagaagaaaa tacatgtatt ttccagcaat tatgcgctgt
    57961 accacagcat gagccaacgg gttatggctg ttctggagtc gctttctccc gcagttgagc
    58021 cctactcaat tgatgaaatg ttcattgatt tgcgggggat aaatcattgc atctctccgg
    58081 agttttttgg tcatcagctc agggaacagg taaagagctg gacaggactc accatggggg
    58141 tgggcattgc gcctacaaaa acgctggcta aaagtgcaca gtgggcaaca aagcaatggc
    58201 cacagttttc cggagtggtc gcgctgacgg cagaaaaccg taatcggatc ttgaagctac
    58261 tggggctgca gccagttggt gaggtctggg gagtaggaca cagactgacg gaaaagctga
    58321 atgcgctggg tattaacaca gcactgcagc tggcgcaggc taacacggca ttcatccgga
    58381 aaaacttcag cgtcattctt gagcgtacgg tacgcgaact caacggcgag tcctgcatat
    58441 ccctggaaga agcaccaccg gcaaaacagc agattgtctg tagtcgcagt tttggtgaac
    58501 gaatcacaga caaagatgcc atgcaccagg ctgttgttca gtatgcagag cgggccgcag
    58561 agaaactacg tggggagcgt cagtattgcc ggcaggtgac gacatttgta cggacatcac
    58621 cctttgcagt aaaagaaccc tgttacagca atgccgctgt ggaaaagctt ccattgccca
    58681 cacaggacag ccgggacatt attgccgccg catgcagagc cttaaaccat gtctggcgtg
    58741 aagggtaccg ctatatgaag gcaggtgtca tgctggctga tttcacacca tcgggtatag
    58801 cgcagccggg attatttgat gaaatccagc cccgtaaaaa cagtgaaaag ttaatgaaaa
    58861 cactcgatga actgaaccag tcgggaaaag ggaaagtgtg gtttgcgggg cgaggaaccg
    58921 cccctgaatg gcaaatgaaa cgggaaatgt tgagtcagtg ttatacaact aaatggcgag
    58981 atattcccct ggccaggctg ggttagttca gtcatctccg aacatatttt cagcgtttct
    59041 tctggtctgg tcttcaccgt catttctcga cagactctgc tctgtaagag gagtactgca
    59101 atccacagac gtatggagac cagattgttt ttccagtaat tcaagcaatt tcttttcaac
    59161 cgaaaaagca cctggtatta cgctggaaat caccttatta atatcatcca gagtcgtatt
    59221 ttctgtcagt aactccgata tcagcaaagg aagacgttta tctatagctg caaacgccgc
    59281 accagtaact aacatcgcgc gcatgaaacg tccccgctcc ttcagttttt catttgcaat
    59341 gagctcatct gctatcttat ctcctgtccg ttcatccggg aaaagttcaa atgagcagcg
    59401 tctggattta ttttcatcac tcattagtca caccaccttt catttttatc attcccatga
    59461 ctaaatcaaa ctgcggggat gagctcagat aaaagcgctc atcaggaacc ttagtcaggt
    59521 tcttcacagc ctcggccaca atttcagccc caccaccaac acacattaca tgtgtatagt
    59581 cggtgaaacg gctgatgaca tcattaactc gtttaataag caacttctgc cgttcattga
    59641 taacattaat gatagactca cgctgttcag cattataaat gcgacgggaa aggtagtcag
    59701 gttcattacg gtgcacaatg atattgtcag cctggaatga gctcactctt gtattagcat
    59761 gtacagccat ctgttcctta accccggatg taatcaggga cacgccgata ttcggatcac
    59821 accaggtttt tgtaatccct gtcattttgc tacgaacatg cgacacatca agagttgtac
    59881 caccgagatc tacgatcaaa agggattcat catcctcaag ccccgcaaga acactgaaac
    59941 cagcgggaat actttcagga agaacggaaa cactgcgaat aacaaatgca tcactcccct
    60001 gcacccgtac ttcacgcatc acattttttt tctttctttc aatgttttgc ttatttttct
    60061 gattgttggc gtcaaggtat tcagagatcg gcaatgtgac gatgacatca acaggctgcg
    60121 ctttcaggcc ggtctgctgt agcgcatgct gaatggccac aacattcaca tcactgtact
    60181 gatagcgtgt ttcagtcgtt acgacagcat cagcacttaa tggatcaaat gaaaacttct
    60241 cgccgtcgat ctcgtaatta gccggagcag catcatccag taaactaaaa gaccattccg
    60301 gtttaaagct gttagggctg attaacgttt tcacgtcacc atcctccaac caggcaagtt
    60361 taatgttggt agaaccatca tcaataaaga ttttcataac cagtctccgt tgctcataaa
    60421 caaatagaaa aaacaaacat acttcataca tgcaatgtat attcaattat aatgaaaaaa
    60481 cggtcttttt gaaaggacaa atgcaacata aaaacacgat aaattgcatg tacattgtac
    60541 acacaacttt aagaccagag gaaatcatag atacaaaaac attatgaagg ttatatgtga
    60601 tacataaatg caatataaaa acatcgtgga catcatggtt caggttaagg gaaaaggaat
    60661 gaaatggtga aactgagagg aaaggaaagc ggtgacgaat catgtcacca taaacagaaa
    60721 gatgtctaat ggggactctt taggtacgtg aagaatcccc ataacgcatt taaaagacac
    60781 acatcagata cccttaaggt atccgatagt ttattgacct attgcaccat cacgttcaag
    60841 cttctctctt atagcctcaa taatatatga gctaaaatcc agagatgttt tactggtcgc
    60901 tttaagttcc gcatgagctt caaaatacga cagcggtatg gccttaagat tcttagcctt
    60961 cgtcctggca gccacgggac gacctgaaac ttcctctgca ccttcatttg gctgagacgg
    61021 ggctgttgct gttacggcag aagaagaagt ttgtaatctg gcagaatcca ccaggttttt
    61081 cagtttttca gcctgtgcac taaatttcgc tttgctcatg agataaccca ggatgtttgt
    61141 tagatactta aaagatatcc attaaaagcc caaaggatac tatatggata cccaatagac
    61201 acaccttact gtttgttatt ttcaatcaga tgattgattt cttcgatcag ggcaataact
    61261 tccttacctc ctgccgaata gcgcccatga acagactcag tgattcccat cccagtctca
    61321 atgacatcct caaattctgc acgagcagaa atgcggctct tcatcagctt cagatgtttg
    61381 aacgatggca ggattgcatc aagcttaggg aatttcttct tgttaggatt aactttacag
    61441 agatacaggt gagcagttat atcggtcccc atgatagagc tgatttcgtc cagtactcca
    61501 tcaaaatgca tcaaaccaat acgttcagtc agggagtctt ttgaaggaac aataacgcag
    61561 tctgcaaagg caatcgcagt acgggtaaga tctgagtcaa atcccccaca gtcaataagt
    61621 aacgtcttat cgctgttctt gtagggagtc ataatttcga ttaactcatc cactgttttg
    61681 ggtactttca cggaaatttc ttttccttca ggtctcagcc ccagaatgat agaaagcccg
    61741 gtatgagtat ccagatcaac tgtatcagac ttcatttcac caacaatgtt tacggatgaa
    61801 gttgtcttac ccacaccacc tttgggatgc gtattagcaa taacaggcat agtgtatctc
    61861 cagaatccac atgtaatacc cataaagacc ccataagaca cctttcggat atccaagagg
    61921 tactcaatag atgttttttt actctatacc ggtcctgcat gtcaacagaa tatgaaatac
    61981 cgataatata tccatgagat atccgaaagg gatttaaaaa agtcagagct tatggaattc
    62041 ttttgagcat agacaccgca tcggactcag gcatcgaaaa ctgcacccgg tgccgtgcag
    62101 ccacatccag tgcaaacacc ttcgtgtaga cctccgttga gctgatggac ttatgcccca
    62161 tcagactctg caggaccttc agcggtatac cggcatacag catatgcatc gcataggaat
    62221 ggcggaacgt atgtggcgtg accgggacag agaacgtcac tccatcagcg gcagcggcgg
    62281 caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag atccgcgctt
    62341 tctctgtcct gcctgttcgt ttattacgcc gttccatggg gattttcagt gtggccacca
    62401 tcgtctgcag ctggctgacg taccaggagt cggagagcgg aaccagccgg tgagtctgct
    62461 gcccggcggg cgttcttcct gccgtcctgg cggctttttc ggtccgttgt ttcagggtcg
    62521 caagctgcac aaacggatac ggaggcgtga gcgaaaaatc cccccgcgtc agcgccagtg
    62581 cttcattaat gcgcgcaccg gtgttccaca gtgtggccag cagcatcttg cggcgcagat
    62641 ccgggacgta atggagcagg gcgctcactt caggggccag caggtatttg ggcagttcat
    62701 catgaaccat cgacatctgg cggagtgcca gcgctgccgg ataatcgatg gcaacaggaa
    62761 gctgagcggc atcccgggca tggtggatcg gtgccggcag gtgctgcata tcagtgttca
    62821 ctccacgggg aaacgtcggg caggttttcc acaacccggt attttcccgg ataacggagc
    62881 tggttaccgc cacgcgtcca gacggtgaca acaaaacccc ctccctggtt gttttcgggg
    62941 agcaggcgat cgcactgctc actcagcatg tggatcacga acggcgcatc gctgccgtct
    63001 tcaaatacca gttccagcgc atcgcggcca tggagcgttc ccttactgat gatgacgtac
    63061 tcagctcccc gcatctccct gagtaaatgt tttgccgcat ccgggacaag cagcctggct
    63121 gcgccggcat tccagctgag gtagacatat ccggcctgcg cctgtacaga ctgccagtaa
    63181 tccgttctgg cgacagcctg accttcattc tcaataaaaa acatgggtga ctcctttttt
    63241 cttttccggg cagggacggt catgcgataa cgtcatccat catcactgcc cttaccgtca
    63301 cgccgtctga taacatcatg ccgttatcat catcaccatg ataacaagca tccatataac
    63361 cacgcagtta aattaccgga gagccgtttc cgtcagatcc gactttccgg aatatcacaa
    63421 ctttccggaa gtgtaccacg atgaggaaat aatcatatcg ctgttttccg cccctgcacg
    63481 gcgcatcaca ggcaagggaa ataacacaac atatagtgtc atcagcagct gaatgcagca
    63541 ctacatattg tgcttacagg aggtggcgtt gtgtgggggg agaggaagat ccgtgacctg
    63601 gggttttcgg atctcagaaa aggaagtgaa cagaaaacag acagcgaagc atacgaatta
    63661 taaaaacgta tgcttcgcag tatttaaaga cactcaaaac caggatagag tataagtatt
    63721 gcagcaatgc cccctcacat gaaaaggtac gcgggatgag tgaaaaagaa catgacatga
    63781 caaacgacgg tggagagagc gtaacgtata ccctgcggaa catccccgct gacaccgacc
    63841 gggtgatcac cgacctggca agccacgccc gtaagccaaa ggcgacgttt ctgcgtgagt
    63901 tcctggaaga cagcttccgc gacgtcatcg acagctttgc cctgaagaac ccgctcatcg
    63961 cctccctgga tgaggagctg gccagttatc tggacgctaa ggtcatggag cagcgctacc
    64021 agagccattt tatcacccgc tggaatcagg aataccagaa gctgctgggg atctcgacgg
    64081 aagaggagct ccgccggttg gtactgaaca acaccccctt cctgcaggtc cgtgccgacc
    64141 aggtgcttaa ggggtggaaa aatatcccgc gcgggatctc cctgaccttc tccctgtttg
    64201 ccgagatcgc cggccgggac agggaaacca tcgaccaggc ctggaaaaat atcttctact
    64261 cgcaactgag ggaaaaaaaa caccgctttt accaagatat cgaggccatc cgcgccctga
    64321 aaaaactgcc tgccctcacc ggcgacagct ggacgcggga tggcatcacg gtgcgtatct
    64381 accgcccgga aaattacgcc cgcggcgcat ggcgcgttac actgagcctc ccggaaaatt
    64441 acgccaccca gatgtggaac atcccgttcc cggagcttga ataccgcctc tttaccgccg
    64501 atccgggcta cagcgccctg atcagcgccg aacccgacag gtgggacaag gccttccgtt
    64561 ttgtggacgg ggtgtgtgag cttcaccttt acaccaacgg tgtggaagaa gatcacaatc
    64621 ccaccccgct cggggatgtc gctcaggcgc tgatcaacgt ggtggaagaa aacctgctgt
    64681 aacggacccg gatctgcggg cacaactgca tcatcaggag gatacaatga aaggacgaca
    64741 gagccgctat gttaccggcg gagagagttt cgcggagatt gcccgtctcc cttcaggggc
    64801 ggtggtgagg ctctgtctga acaccggtct tgaggatgcg ctgcgggagg cctccaaatc
    64861 gctcaagtca gccttcaccc gttccgggcg aaaatgccgg ctgtcagcgg gtacggcgca
    64921 ggggccgttt accggacgcc ggcagggcgt ggccacacat ctcttcgtct cggtactctg
    64981 agggggcagg gggcaaaaaa agtaaaaatg tattcgccag gttgcccgga ggtgaaggaa
    65041 aatagacata cagcagaacg acggatagca ctttttgcta aatggacatc agtattacta
    65101 tgctatagtt gctttaatgg ataagtgcgc cttgacaaag gcggtgattt ctgttaacat
    65161 tactctcata gtattgttcc gtcccgctcc accccaacaa gatccgttta tttcccgcca
    65221 gactggttat caccattccg gcccggattt ttttggattt ttttccgggg agcccccgga
    65281 acgagcttaa aatcggtatg acaaacagga ggatgcgaat gaacacatca taacagagct
    65341 gaaagataaa acattctgta cggcattaac agcgttcacg tgtgtgaggc gccgggtgcc
    65401 ttttgactta aaaacgaggt tattgagatg accaaaatca agacagttac ttttgtaaat
    65461 acttacccgg gagggttcta tgaaaaactt gttagacacc gagggaacgg ttctattccc
    65521 attccagact gaaatccatt ttatttggac gattttctcc accgttaaac gcctggttat
    65581 cggaaccagg gaccatattt gccagaagca atactggagc gcctgtctct gtattttgct
    65641 tcttatggcc tatgtgggtc tctgtgctgc ggtggtctgg tttgtagtgc cctggctgaa
    65701 ggtctttata gtgtcgaaat ttgcggtttc ggcactatgg gtcacgccag taaagcgcgg
    65761 actactctgg ggtatcggta aagtggttac cgccacttgc cgaagattta ctctgctaaa
    65821 gtaagtagcc gcaacgctac acgaactgat ggtgaatgtc aacagatact caccatctcc
    65881 ttacggcggt ggtccctgtg accactgccg ctttcgcgtg ggtgcaacac ggcaaaactc
    65941 ctctgtacaa caggggctcc cgccgtcatt ttccggcaca ggtgaggccg gaattcggac
    66001 taaaacgtaa accgcgggcc agtccggtag cgttcactat cggccagcat tctctcaacc
    66061 agagagaaat ccttttcacc gcagaacacg tactgctccg cgaactccac ctgcagggaa
    66121 ttgtaatgcg tgacatgctc cggggtaagc ggcataagaa gatagtccgt atacctccgg
    66181 atatgttcca gtctttcaaa aggccgaatg tgacggggga taagatgcgg cgccctggcg
    66241 agcagatgtt gaagatgctg cttttgacga accatctgtt cacggatgga ggggcaatac
    66301 atggccagca tcagcgtcga tgacaggggg aggtaaatct gtatcccgcg aacggccaga
    66361 ccaagattac cgtacggacc gaagtcattg ctgttcttaa gcactacggg attgtctgat
    66421 acgtaaaacg gacgttctgg cctggtttcc agaaggtacc agtccttttt cagcaggaga
    66481 tcaatatgtg acacctgctg gacgagctgc cgcagaaaaa tatccttggt atcgcccccg
    66541 gacgacaggc ccagggtttc catggcctgc tctttagtcg agccgatggc ctccatctta
    66601 tcggtaagca ccgaaatcat gcctgagata cgctccagtt caccgaaggt tcgtgcgcgc
    66661 tgcacggcaa caaacacggc cagcctgtag cgttcatcat ctgtcagcct gcgaatatcc
    66721 ctgtgcgcca gcagggctgc aattaccggt gccgcgtggt gctcgtaaat ccccagcagt
    66781 ggttcaagac tcagacggtc cgggtgatta tcgaggttgt agaacgtgtt gcggacggtc
    66841 gcgtcatccg gcgtggtggc ataggcgcgc ccggcagcct tgtcaaaggc gtataagcgc
    66901 tgccgcttac cctttcccgg cgtactgaaa tgcttcagta aaaaacgggg aaccgtatgc
    66961 tgcctgacca catcggtctg cagattgaat ggatcggcca taactttatc tccctgaaaa
    67021 gccacgcaag cgcatcggtg catctgtatt gacggcatgg gcagctggtt tgaagcttag
    67081 cattagaaaa cagaaccgtt cgataatacc tgtgtttaac gccctgtcag taccgatgga
    67141 atttaaatct ccggaaccgc cggaaaccgg atctgcgcgg cagaaaagac gcaaatgata
    67201 tgataataaa aaatacacat aaaatcatat cagatatgat tttcatcatt gatgataaaa
    67261 ccatatctga tattatcaga aatcaggagc acacaaatca ttcaggatat gattattacc
    67321 tgtcagaata ttatcagatg gagcgtgcag gatggatcag gaaatgacgt tttcactcag
    67381 ctatgaacaa ctgacccggt ttgcggaacg acgcatccgg gaatgcaatc tggacagcca
    67441 gggcgcgatt tatctctgcg agtcagccaa agccggagcc gtgctgatct tctggcatga
    67501 actggccata aatggctatg cctcaatgaa tgccataaag agacaggagc tcattgacgc
    67561 cgactttcag cgcctcagaa acctcatctg gccggaggat gacaggtaat gacccagagc
    67621 cgccgaccgt cgccactgca gcggagagtg ctgattgtgc tggctgccct tgatgaaaaa
    67681 cggccaggac ccgtgctgac gcgggacata gaacgggtgc tggagcgaag cggggaagcc
    67741 ccggtctacg ggccaaacct gcgcgcctcc tgccgtcgtc tggaggatgc cggctggctg
    67801 cgtaccctgc gcgctccgaa cctgcagctg gcagtagagc tgacggacgc cggccgggcc
    67861 gtggcacagc ctctgctgct ggcagaacag gaccgcctcc gggcagaaca gcgtgcggca
    67921 gaagtcgtcg tactgccgct ggtcccggca gcaggtctgc ccgcagatgg cacttctgcc
    67981 actgatctgg cggtgcaact gaacggcata acgtatcagg cctgccgtgg ggattttgtg
    68041 gtgcgtctag acggcagcac ctgtctgcag ttatggaata aagaaggcag ggtggtacgc
    68101 cgggagggcg atccgctgga agtggcgcag tggctgcagg cctgtcacga tgcaggaatg
    68161 gaggttcgcg tacagataaa tgaaagtgcc gctccgtaag aacacgcctg tgcacggttt
    68221 acacatcatc ccatggtggc agccggttaa ctttcctgcg ccaccagtgg aaagaggcca
    68281 tgcacagtcc ggacaaaacc cccccccgtg atacttataa taatggcctc acctgccacc
    68341 attcctgacg gcccccagta gataaaccac attgcacatc cccaggaaat gccccataag
    68401 ccccccgtca gcaccgtgac ctgccagaac ggcataaagg gtaatggcgg aagccggata
    68461 cccagccgcc agagaatacg cagaagagga ggggcataat tgctccgcca catctttttg
    68521 ctgtccatca gggcaatggc ccgagctttt ttctgctcaa aggtcacagg agcacctccg
    68581 ttccatgatg taaacgtgac acagcttatt gttattgatt attattatca tgctgtgatt
    68641 atgttatcac gcaccggtac acgataacaa taaaggagaa accagatgaa actggatata
    68701 tctgtaaagt atttactgaa aagcctgata ccaatcctca ttattcttac agttttttat
    68761 ctgggatgga aagataacca ggaaaatgca agaatgtttt atgcgttcat cggatgcatt
    68821 atcagtgcca ttacttttcc tttttcaatg aggataatac agaaaatggt aataaggttt
    68881 acagggaaag aattctggca aaaagacttc tttacaaatc cagttggcgg aagcttaact
    68941 gcaatatttg aattattctg tttcgttata tcagttcctg tggttgccat ttacttaatt
    69001 tttatactct gcaaagccct ttcaggaaaa tgaaagggca tcattatgtg tcagatacca
    69061 ataagtttat tgacctgctc aataaactta tcattaacaa gcgcactcac agccgccata
    69121 ataattgcaa aacccagaat acctacaggg gttcccagca tgacggaaaa cgcccatgct
    69181 gtaactgctg aagcagctct tcctgctgcc agtgattcaa gttttacaaa aaatggacgc
    69241 cagttgtccg tttttacagc cttttgtaac tccacagcaa catcataacg atcaataact
    69301 tttccggtaa aaccaaatgc cttactgaac agcttaaaat tctccgccat atggacctga
    69361 ttaatagctt ccagggcttt agaaatggcc atgcgatctt gtatgttata tttcttgttc
    69421 agattattac ggaatttatc aaaagcgttc agtgcatcat ccacactctt aatctgtttc
    69481 ccttgagata cagaagccag ctccttagca agttcggatg cttgtttacc gaactcatca
    69541 tatatcgttc tgtagaaatc agaggtgagt tttatggcat cctttaccat atttatttca
    69601 tccctttttc ttttctcttc agctattttc tgattgatgt cagcaagctg gctacggata
    69661 ttctcttttt ccttcaacag ggcattaaga gcgtcatttg catgcttttg ttcatttgtt
    69721 ctggcactga cgttatttct cgccgaattt atcgcagatt cagcagaggt gattttattt
    69781 ctggcatcaa gcaatcgttg ccgtaactta tcccattcag caacttcagc attataaatt
    69841 ttcttgtcac cttccacatc ctctgtcacc accggattcc ggtcattcag aatattgcgt
    69901 ttatagtcca gaccggaatg ggtcagaaga taattcaggt ttgcacggtt atcgatgacc
    69961 gcgctggttg tgatttttat cgtaccggca aacctcggat caccactcac aacaatagaa
    70021 tgatttgttg atgaacgccc cgggaatttt tcaggggaaa ctatcgtttt gccgtcaggc
    70081 gtgtttttct gttgcgtcag ctgttgttca gctctggtta atgcattacg ggccgtctta
    70141 agcgattccg tcaccgaact gagtttattc cgggcctctg aaatggccct gtcattccgt
    70201 gtctgctgcc cgagcagacg ggcctgttgt gcatcaagaa cggcaagggc tgccccgcca
    70261 tttttttcca gctctgacaa ccgcgtgtcc agaatacctt ttctgcgctc gtattcagca
    70321 agcatatcag cggccttctt gtctgcgttc tgcgcatcct tctgccgttt ttcagcctgt
    70381 tcgattaacg acctggcctc gcggggagat atacgaagag aatcagcatc agcaaacagc
    70441 atccggctct ctgtatgcag ccggagggca tcatatccgg cgatttcagt acggaatccg
    70501 tattctctca gctctgcctg caggagtctg aactcctgtc gtgtaatatc agcggcatct
    70561 gcgtttccgg cacggagtgt tttcagtgtg ttttcatctt tttcccttgc agcaattgcc
    70621 gcagaaaggc gttttccagc ttcagtacgc ttattctcat tctctttctg ctgcgcgctg
    70681 agcgtgtttt tgtacgctgt gatttccttg tccctttttt cgatatcact tcgggttggg
    70741 gaatcatcga cccactcgtt tccaccccag atggttttgt tcccgaagga actccatgca
    70801 ggcggggtac catgaaagac atcgacacgt ggagggttta cataaatatc aacggccata
    70861 atttcatggc catctgaatc atatcccagc gattctgcac cgggatttgt aatacgtaca
    70921 gggtcagaca ttcggtatct ccttcatcct ctgaggaaaa caaatgaatt aactgcttac
    70981 gtatatggat acatatacag tactaattta ttaaggagaa aatggcatgt caagatttaa
    71041 tgatagtaat tatcattaac atgaagtggt gttataccgt tcagaaagcc tgctgacact
    71101 gtggcagcat cacagtctgg gtattctctg gtgcaataca aaaaccggac tttcccgtaa
    71161 caacagacaa tgttacggga aaagttacag aactgagcca caaaatatct cagtacctga
    71221 gatgcataaa aacaccgtac atatgtttct taaaaggggg atgatcgtta tccagccatt
    71281 tcggtggatc ggtatgcaga acaccaatct gcttcatgcc ggtatgctgc ctggattttc
    71341 tcgcgcagta atcactgtct tcatatccca tcgtttcgat gaccactctg gccgtctttc
    71401 cgtcaggagc ccttgctgtg acaatgaaat ccggcagtac atatttcttt tcaccattaa
    71461 cataaacttc tgtatcaaac aggggccttt ccagacttaa ttccggcgta cctggctttc
    71521 ttttaagcca gctggccaca ttcagaagag cagtgagcgt gtttcgttcc agtttgctgt
    71581 ccacaggcac cggacatgtc agttgataaa gagcatgagc atacccttca ctgcagatga
    71641 ttctgccatc ggcactccgg caaaaagcaa gaataaccca gtaaggggga cgtattccgc
    71701 cctgcgcttc accattaata ctgatccccc gcacaggtct gatgacctga atattaccgt
    71761 tcgcccagtg aatttccaca gcatcacgac agatgtgctg cgccataaaa atctgaaaga
    71821 acatatgctt tcttctggca ggccagtgtc gttctgaatt ctccagcgtt tccattaacc
    71881 gctcctgaga cctttcagac attcctggcc ggaaatggat aatttcagat aaggctcttc
    71941 cccgaatata ctcctgctgt agtgtaacct gccgtatggc atcaagacac tcacgataat
    72001 tagtccggat acggggatta gccagggaat ccagtttatt cagccctgca tcttcaataa
    72061 gcgacaggag taaacggcca aggcgcggac gtttcttgcg aacggaggag cgctcaccat
    72121 tatggtaagc agagcgggcc tgcagtctga taacagaatt actgtcatca ggtggtaaaa
    72181 agtttctgta atttatacgg gtgctaacgg gatgccggct tgctccgaag gatgtttcct
    72241 cttcatttcc ggataactga cgatacatgg ggcattcagg tgcatgctca tgattaaaac
    72301 tggacagata aagagtaccc gtattgtttt taagagcagc aaaattcatg gccggcgaat
    72361 ctcccggaat acagtcacac tgtaaccatg catcatactc acgcatttcc cggagaagat
    72421 cattggccag gttatgttgt gccatggttg ctggcctttt cagtccacag accgtcttta
    72481 ctgcagtctc ttcctcactg ctaagctccc gaatcacttt gccccgggga ttcttcttta
    72541 caagtatcat tggcataatg aaagcactcc ttatgtacat gcttaaacag taagtaaaat
    72601 atacaaagaa ccccccatga aacaaaggca gagatgaact gtgcgccccg atatcttctt
    72661 ctgatatcct ttcaaatgaa ttgtttttat ttatatcatg acaacattaa agtgagagaa
    72721 tatatcaacc atgtttctgt cagggttcag tagaatatca attcgactgt cacgccataa
    72781 ctgtaactac ctcgagtata aatattcaga gaatggactt attagccatg aaaaatcacc
    72841 tgatgggaaa tttactaatt tatggattgt gatattatta aagagtaaaa ccagccagcc
    72901 gaggtattta tgggaaaaaa tcaggaagaa aggaaaactc ccgtcattgt ggtgaaaaaa
    72961 cgtcgtactt tttcgccacc gtccttatcc gaaaaaacag atattatcgc acctgtattt
    73021 actgaacaga cggcagaatc agcccccgcc ggaataaatt catctgccgt cgaaacacat
    73081 atccccgaag cgccagccag gaagaagaaa aagaaaagac accgttttcc acgtccctcc
    73141 cactggaccc gtgagtatac tcacgagtgc gtggaaaaaa taaaggccct gtttcctcat
    73201 cttcgtgctg aaggtggtgg atttattccc ctgaaaattg gcataagcaa tgatatttcg
    73261 gcatttctgg cagaacaccc ggagacagaa ctgaccatgg acgaatggct ctgtgcagtg
    73321 tcctgcatca catcccgccg cgtatatctg cagcggacag ctgtcgccgg cgtcccacgt
    73381 tatggcctgg atggtcaccc caaagggcag gtctcagact ctgaagcaca gagtgccggt
    73441 cgcagactgg cgacattaga acaaaagtgg ctcaggacgc aggcacagca ggagaatatt
    73501 tctgggcaat aaacactcct gataacaggt ggacattacg tatcagaaaa aatgggaaga
    73561 gaatgttatg tgtgattcag aagcggagaa gattttattc atcctgaata aacatgaacg
    73621 ggcatacaga cgcctgatcc ccgtatatat ttttttagct atagtattat ggattttacc
    73681 tattctcctg attgtatacc tgtatttctg tatagttgac aatgagtttc tcagaatcgg
    73741 tttctgtatt gcatggacag catactactt tctttcgctt ttcaagtatt ttgtaaaata
    73801 cttctttcct ctcgatcgtg aagatttgct taacctgatg gatctcgcca aagattctcc
    73861 tgatgcgaaa caggagcttc ttgagcgtct tttgtccgga aaaagaatga ccgggcggga
    73921 tgaaatggat atcaagcgca ttctaaaaac aaaggaagca cttgataaca tcaggaattt
    73981 tattgataaa cagcagttaa cgaatagtga tactccgaag aaaagcacgg tcccaaatga
    74041 agataaaaaa ggcataagat gatgctcaca ggaaatggaa taaacactgt aactgtcaat
    74101 ggaaaagtga aacacatcac agaactggat gatataacac tttgtttaga gtggacaaaa
    74161 ctacgagagg aaaacaatcg tctttatgaa attaataacc aggcaaacag aggatggcgt
    74221 ggtttcatcc taagattgat aggggttaac ctgccagata aaaggactga gtttacacaa
    74281 cgtctcttat tgacgcgcaa aatttcgggt tcggtcatga aaaagtaaca aaaccgcgca
    74341 acagacataa atatgacagt agccatgaaa aagcagagag agacagggtg atacagaaaa
    74401 gtaactattt tttagctata gtattattgg ttttacctat ttttgtgatt ttatttctgt
    74461 atatttgaca atgagtctct cagaatcggt ttctcgaagt gacgagcgat cagaatggag
    74521 acaaataaat tcgggaaacg aaccttcacg gtaagttttg tcacatttat catccctaaa
    74581 catgagaatt atcaccaaaa ctaaccaccc aaccaacagc agcgccggga gcagcattat
    74641 aaaatcatgt acagagttgc gtgacgacat gataagttct cattctcaat atgttcttta
    74701 ccagattata ccagtgatac tttaacgcac catcattaat gctaatgtat gcatacatta
    74761 acattaatga tgaaggagaa gaacaacaat ggcagtcaac ccacttattt tttggatttc
    74821 ttaattttcg tctgataaag ccgctttacc cgttcaattt ctgccagcgt tgcggaccag
    74881 tgtagttcgg cggcatgacg atatctggca gccatttttt ccaccatctc cggattgctg
    74941 tccgcgctgc ggtgctgacg ccccttctcc ccaaaaactt caacaaccag ccactggctt
    75001 ccggttctgc cgaacaatgc cttgcacaaa cgtctcacag ttagcggggt taaatctgga
    75061 tcgggatcgt aggcaatggt agcaaaaata aagcgcagca tttctgatgt cttgtaacgc
    75121 tttacgctgg cagccaggcg tgaggcacgt tgcggtgctt cccagcggtc gttactgtaa
    75181 ttagccactt cagccagata atccttttca agtttttcag ccgtgtagca cctgccgcct
    75241 aaaaaaagcc gcatggcctc cctggttttg tctctcacat atcatccctc attaatgcta
    75301 atgtacacat acattagcat taacatgctg acagcaacaa gcggcggatt tacgtctgga
    75361 taacgtaaat gattgcggta tacaggccag gcggtcagat tttcagccgt gcctgttcct
    75421 gcagcagttc cagatgatat gcagcaatag cgtttaccac ccctgccgtc atccgtgcgt
    75481 cggcgatcgc tgagtgtgcc tgcccttccc aggtcagtcc ggcctgactg gcagcacagg
    75541 ccagtgaaat cgtgccatag cggttggtgg ccccataata tcctgcagcc agctccatcg
    75601 cacaatatac cgtcagttct tccagccagt cagccggatc gctatgtgcg gcagcagtct
    75661 gtttcagaat gcggatgtcg aaccgggcat taaagataat tactggtcgg tccccgattg
    75721 catgacgcag ttgccgcgcc acatctgtcc atgatggtgc gccgcagaga gcctgctcac
    75781 tgataccatg cacggcggcg gcctgcgccc cgatggcgac cgtgggcttc agcctggttt
    75841 caaataccac ctgaccggcc gcatcggtca ggccgatttc cagggcttcc gcggtattat
    75901 ccagtcctgt cgtttcagta tccagaaaga gtggtgccag actcagccag tcatatgcct
    75961 gccgtgccat ccggccactg gtgctgttta gtcgggataa aacggaaagc cgctggccag
    76021 ccagctgttg tttactcgtc agagggcgtt tctcacgcat cggaacacag tctgcaattc
    76081 ggtatacagc gaacctgcca ccataactat ttttatacca cttaaccggc tcagcaccgg
    76141 gggctggttt cattctgaat tcatcgcgca aacgtccttt cgagaaacac ctgtcttcgg
    76201 caatcgcctg cgcacgtctg gtggccagct catcattatt catggttatt gcatctcctt
    76261 tccttatatc cggacagccc ctctatggcc acttctgtgc agtatgcaac acgcgcagga
    76321 tatacacttt tccccactgg ctgtcgacct cataaaccag aacaaaatgc gggtgaataa
    76381 ccagttcccg ggtaccttcc acacgaccgt tgcggccttt atacggctgc ccggccaggc
    76441 tgctggctga ataaccaatc cgttcgtcca gttcaatggc ggccatcagg ttctgctccg
    76501 caatgtactt acgaatgcgc ttcctgtcct ggctggccag catggtccag aatatctcca
    76561 tcatttaccg gcaaccttcc tgctcatttc atcccgccat gcagcagcgg aagattcgac
    76621 catgtcatgg ggaagcacat cgccgcgctc gagttgctga cgtcctgcct caacctggtc
    76681 acggaaccag gtattatgct gccgttcaac ggtctggcgc ataaaatccc gaattaactg
    76741 agagccatta cggtccatgc tttttgcagc ggccataaaa gcatccttca gttcagcgtc
    76801 gattcttaaa ctcatattaa cctgtgccat ttatcacctc gtcattacta cgtatataca
    76861 cagtatataa cgattcagat atcgtcacaa gcgaagcgcg gaggccgcag gccggaggca
    76921 ttagtggccg ccgcccgaag gggcgagacg cgtagcggct cgatgcgcag cacggcagaa
    76981 cggccccgca ggggtaatgc ccagtttaat tcaacgtgac agtcacgtgg aggaaaaata
    77041 tgaatgaccg acagcgtgaa caagcccgta ttcgccaggc ccggcgccgc gcgcgactca
    77101 aagaggaagg cgctagcgtg acagtcacgc taacaaaaca ggaagaagca atgttacagg
    77161 agctttgccg ggttcgccgt ccaggacgaa caacccatgc gtcagtgggt gttgagcttc
    77221 ccgtttcagc tgcgtttcct gtttgccagc cggcccgaga tcatggggtg ggtgctgggc
    77281 atcgtttacc gcgtcattgc cacgcacctg gtcaagaaag cgggccatac ccaccaagtg
    77341 gccaagacgg gcgcggtcac cctgatccag cgttttggat cggcgctcaa tctgaatgtt
    77401 cacttccaca tgctgtttct cgacggtgtg tatgtcgagc aatcccacgg ctcagcgcgt
    77461 ttccgctggg tcaaggcgcc gaccacccca gagctcaccc agctgacgca caccatcgcc
    77521 caccgggtgg gtcgctatct ggaacggcaa ggcctgctgg aacgggatgt cgaaaacagc
    77581 tatctggcct cggatgcggt ggatgacgac ccgatgacac ccctgctggg gcactcgatc
    77641 acttaccgta tcgctgtcgg ttcacaggcg gggcgaaagg tgttcacttt gcaaactctg
    77701 ccgaccagtg gtgatccgtt cggtgacggg attggcaagg tagccgggtc cagcctgcac
    77761 gccggcgtgg cggccagggc cgatgaacgc aagaagctcg aacggctgtg ccggtacatc
    77821 agccgcccgg cggtatccga gaagcggctg tcgttaacac gaggcggcaa cgtgcgctac
    77881 cagctcaaga cgccgtaccg ggacggcacc acgcacgtca ttttcgaacc attggatttc
    77941 attgcaaggc tggccgccct ggtaccgaag cccagagtca acctaacccg cttccacggg
    78001 gtgttcgcac ccaacagtcg gcaccgggcg ttggtcacgc cggcaaaacg gggcaggggc
    78061 aacaaggtca gggtggctga tgaaccggca acaccagcac aacggcgagc gtcgatgaca
    78121 tgggcgcaac ggctcaagcg tgttttcaat atcgacatcg agacctgcag cggctgcggc
    78181 ggcgccatga aagtcatcgc ctgcattgaa gaccctatag tgatcaagca gatccttgat
    78241 cacctgaagc acaaagccga aaccagcggg accagggcgt tacccgaaag ccgggcgcca
    78301 ccggctgagc tgctcctggg tctgtttgac tgacgagcct gaaggccaac gataccaatc
    78361 aaaatgctgc gttcacagcg ccgcggcagg gatccgccgt gctggttgtc ggaaaaggag
    78421 ccgctagtgg gaaagaggag ggtaaatttt cagcgttgct ggctccccgt cagccggatt
    78481 gggttgcatc gcaggggtgt cgaaagagtc aactgcggtc caaagctgtt ggacttgggt
    78541 gaaaagggcg tttattcttc ctatacgttg tcggcagcgg gccaaaaagg aatacgtcca
    78601 tgcccatcga ggtgaaaccg gctgtgagcg cgggttcaag catatagccc gacaggcgcg
    78661 tatccttgcc gatcacgaca cgatggcggt ggtcaccgcg acgaaagaca cggccagccg
    78721 ccatgccgac gcgcaaggcg gtttccgccg tcatcgcgcc ttcgttggct ttgccacgaa
    78781 taccgtctgt gccgaaatat ttgcgcacca taaggtcgat tatcctgtcg tcgggtcgcc
    78841 ctcaaagggg acatgcctgc tgaaccgcga atatagagaa atatcccgaa tgtgcagtta
    78901 acgaattctt gcggtttctt tcagcgccgc caataccgcc agcccgtcgc gcaaggggcg
    78961 cggctcgtgt gtgcggatga agtcagctcc acctgcggcg gcggcaagct ctgcagcgag
    79021 tgtcgcggcc ccgacatccc ccggaccacg gcctgtgagc gcgcgcagaa aggatttgcg
    79081 cgaaacagac agaagcaccg gcaaatcgaa gcgcagccgc aattcatcga accgcgccag
    79141 caccgagagc gaggtttcgg gagcagcccc cagaaaaaac cccatgccgg gatcaaggac
    79201 aaggcggttg cgtttgatac cggcacccgt cagcgccgcg atgcgcgcgt caaagaacgc
    79261 cgcaatgtga tccatgatgt cgccagcggg tgcctcgcgc cgatctgcct gcccgtcttg
    79321 caccgaatgc ataacgacga gtttggcaga tgatttcgcc aattgcggat agaacgcagc
    79381 gtctggaaaa ccgcgaatat cattgagata ggccacacca cgcgacaagg cataggcttg
    79441 cgtcgcgggt tgataactgt cgagcgagac gggaatgcca tctgccttga gcgcgtccag
    79501 caccggcgcg atacgcgcga tttctgtgtc ggacgaaaca ggcgcggcgt cgggattgct
    79561 ggatgccgga ccgaggtcga tcacatctgc cccctcggcc atcagcttac gcgcctgcgc
    79621 aatggctgcg tctggcgcca gataccggcc tccatcggag aaactgtccg aggttatgtt
    79681 gacgatgccg aaaatgatga gcgatttatt catgggggct tctataataa taataatcga
    79741 gcatgagtct catacggatg ctcgggtcga aagggaatcc ccaggcgagt aacctgtttg
    79801 cggtgatcca ttagctgcag gagcagaata gcatacatct ggaagcaaag ccaggaaagc
    79861 ggcctatgga gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt
    79921 ttctgagcat ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat
    79981 aaaagataaa aatgtcttgt ttacaataga gtgggtatcg gagacgaaga aggacaaggt
    80041 acatgctgac cgataccaag ctgcgcaatc tcaagcccag ggacaaactc tacaaagtga
    80101 atgaccggga aggtctctat gtgggggtcg cctcagaaaa cggaatctat ggtcactccc
    80161 gtttttgcaa caccgatttt gacgacaagt tggcttgctt gaatctatcc ggcgtctgaa
    80221 tgggatttta ttcccgcgcc tcgatgagtt ccgcgcctga tgaacctcca gaaaatatac
    80281 ggcttcaatg agcctttccg ttttacaggt tcctcaacag gccggtgggc cgttagtatc
    80341 atcaatatca gtattcgcaa aaccagatga atgattgttt aaactggtgt atttctgcct
    80401 ttatgcttcg taagtttgct gtcgcgccgt cagtgcccag gctattctgg ccagcttgtt
    80461 tgccagagca caggtgacga caaagttgct tttccgacac aacaactccc tgacccagtc
    80521 ggccaacttg ccagactggt gttccagttt ttgtatgaat accctggcac actgaaccaa
    80581 caaagttcgg atctttttgt tgccccgctt gctaatccct aacaatgtcg tccgacctcc
    80641 cgtgctgtac tgtcggggta ccagccctgt tgccgccgca aagtcacggc tgctggcgta
    80701 ctgcttcccg tcgccaatct cagttgaaat agtactggca gtcagcgttc caacgcaggg
    80761 aatactcagc aagcgctgtc caacctcatc ttcgtccaac tttcgtttca actgagattc
    80821 cagatcttta atctgctcaa caagatagtg ataatgctgt tgtaatttca gcagtaactg
    80881 gctgagataa agaggcaaac tactgtcctc aagaagggta ctcagtcgac taataacggc
    80941 agcacctcgc ggaacgctga taccaaattc cagcagaaaa gcatgcatct gattagttgt
    81001 tttcacctta tcctgaacca gggattcacg gacacgatgc agagctcgca ttgcctgctg
    81061 agattcggtt ctgggctgca cgaaacgcat agatggacgt gatgctgctt cacagatagc
    81121 ttcagcatca acgaagtcat ttttgttgct tttaacggca ctgttgcaaa gttagcgatg
    81181 aggcagcctt ttgtcttatt caaaggcctt acatttcaaa aactctgctt accaggcgca
    81241 tttcgcccag gggatcacca taataaaatg ctgaggcctg gcctttgcgt agtgcacgca
    81301 tcacctcaat acctttgatg gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg
    81361 cgccgattat ccgtttcagt ttgccatgat cgcattcaat cacgttgttc cggtacttaa
    81421 tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc
    81481 gaccataggc gggcgcttta tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt
    81541 tgttgaggat tttacccaga aaccggtatg cagctttgct gttacgacgg gaggagagat
    81601 aaaaatcgac agtgcggccc cggctgtcga cggcccggta cagatacgcc cagcggccat
    81661 tgaccttcac gtaggtttca tccatgtgcc acgggcaaag atcggaaggg ttacgccagt
    81721 accagcgcag ccgtttttcc atttcaggcg cataacgctg aacccagcgg taaatcgtgg
    81781 agtgatcgac attcactccg cgttcagcca gcatctcctg cagctcacgg taactgatgc
    81841 cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt
    81901 tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac
    81961 tatttgcaac agtgccgata gtttggctgt gagcaattat gtgcttagtg catctaacgc
    82021 atagttgagc ggcgggcgca gcccgtccgc ttgaacgccg agttaggcat cagatgccct
    82081 cggcgcgggt cgatgcactt ttcgcacatg ccgctcaacg caagattctc tcaatcgttg
    82141 ctttggcata tcgaacgaac gcggccgtct cttcgacgcg cattgctagg tcgtcgtcct
    82201 cgctacccag gtacgccgcg cgtgccttgc agatgagggg ccgatgctcg gcaggcaaac
    82261 gctccgatac ccatgcggca gcaacgtcct taggagcaat gagaccagtt gaagcgctgt
    82321 accaaatgcg agcaagagca agaacgacgt tccgctcgtc acccttccaa tccgactctg
    82381 cattccactg ggcaatagtg tcgaaaagcg ccttggagaa atgctccttc ggcaccggct
    82441 cgaaaaacgt ggctgcggat gggcctagaa gcgcaaggct gtgttgcctc gccttggtca
    82501 gcaaaatcgc aagatcgtga tccagaacgg caggctcgaa cgttccggaa aggatgtcgt
    82561 ggcggagcca ctcaccgaac tgaagctcac gccgcgccgg atagcgccaa ggcactactt
    82621 cgcttcgagc gacaacagtt agctccagcg gtcgccatgt tccgccatcg cctggcggtg
    82681 atgagacttt cagcaaatcg agcattagcg cctgccggag cgaatcgtta ggtgcggcgc
    82741 tgacggtcac gagcaagtct atgtcgctgt ccggcttcag ccctccatcg atcgcagatc
    82801 cgaacaggtg gattgtgtcc agtgtcgcag ccagatggcg ctcgatcacc gcgcgagcgt
    82861 gggacagctg cttgaaaact tgtgcaggga aaaattcacc catgatgcct aacgttaagt
    82921 tcagcggcag cttttaagtt gcggctttgt ggaatacttt tgcgcagcaa aaccacaaag
    82981 acgcgactta aaagctgtcc aaggagcgaa gcgactggtg ctgcaacgca ttgttagcct
    83041 tttttccaaa tctggtatgt ataatttata ttagacataa aaaactgttc aaaaaccaaa
    83101 ttgaaattct caggcattat agggaatttg atatcacctt cgacttcaac gtgaacagta
    83161 gacaaatgaa ttatatctgc tttttcaata aggctattat agatttgacc cccgccagag
    83221 acatatacat gatctgtaac ttttgatagc tctttcaaag cattttctat tgaaggaaaa
    83281 actaggacgt tttcatttga gcttgaaatt ccgttctttg acactactgc atatttgcga
    83341 tttggaagaa cacccataga gtcaaatgtt tttcttccga caaggagcca ttgattatat
    83401 gtgagcgctt taaagagtag ttgctcacct tttactgacc acgggatatc aggaccacta
    83461 ccgattacgc cattttctga cactgcagaa atcaatgata ttttcaattt aactccctta
    83521 atggctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
    83581 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
    83641 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
    83701 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
    83761 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
    83821 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
    83881 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
    83941 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
    84001 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
    84061 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa
    84121 ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc
    84181 tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcagctg
    84241 tcgcgtgcac gggcatggtg gctgaaggac caggccgagg gccgcagcgg cgttgcgctt
    84301 cccgacgccc ttgagcggaa gtatccgcgc gccgggcatt cctggccgtg gttctgggtt
    84361 tttgcgcagc acacgcattc gaccgatcca cggagcggtg tcgtgcgtcg ccatcacatg
    84421 tatgaccaga cctttcagcg cgccttcaaa cgtgccgtag aacaagcagg catcacgaag
    84481 cccgccacac cgcacaccct ccgccactcg ttcgcgacgg ccttgctccg cagcggttac
    84541 gacattcgaa ccgtgcagga tctgctcggc cattccgacg tctctacgac gatgatttac
    84601 acgcatgtgc tgaaagttgg cggtgccgga gtgcgctcac cgcttgatgc gctgccgccc
    84661 ctcactagtg agaggtaggg cagcgcaagt caatcctggc ggattcacta cccctgcgcg
    84721 aaggccatcg gtgccgcatc gaacggccgg ttgcggaaag tcctccctgc gtccgctgat
    84781 ggccggcagc agcccgtcgt tgcctgatgg atccaacccc tccgctgcta tagtgcagtc
    84841 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca atggaggtgg tagccgaggg
    84901 tgtggaaaca cccgactgcc ttgcgtggtt gcggcaggcg ggttgcgaca cggtgcaggg
    84961 tttcctgttc gccaggccga tgccggcggc ggccttcgtc ggcttcgtca accaatggag
    85021 gaacaccacc atgaacgcca atgaaccgag caccagttgc tgcgtgtgct gcaaggaaat
    85081 cccgctcgat gccgccttca cgccggaagg ggccgagtac gtggagcatt tctgcgggct
    85141 ggagtgctat cagcgcttcc aggcgcgggc cagcactgcg accgaaacca gcgtcaaacc
    85201 ggacgcttgt gattcgccgc cgtcaggttg aggcataccc taacttgatg tcaggcaggg
    85261 ccgcgccgct tcgtcagaat agagtctgct ttcccatttt ttgacacatg cccgcgaagg
    85321 ttatagattt cagcctgaca gaaatgggct ttgaggcaca acggaacaga aagtgcactt
    85381 aagccgcctt caaccaagga gacatcgtgc aggggcaccg catcggctac gtccgggtca
    85441 gcagctttga ccagaacccg gaacgccagc tggaacaaac ccaggtgagc aaggtgttca
    85501 ccgacaaggc atcgggcaag gacacccagc gcccccagct cgaagcgctg ctgagcttcg
    85561 tccgcgaagg cgatacagtg gtggtgcaca gcatggatcg gctggcccgc aacctcgatg
    85621 acctgcgtcg cttggtacag aagctgactc agcgcggcgt gcgcatcgag ttcctgaagg
    85681 agggcctggt gttcactggc gaggactcgc cgatggccaa cctgatgctg tcggtgatgg
    85741 gggccttcgc tgagttcgag cgcgccctga tccgcgagcg gcagcgtgag ggcatcgcct
    85801 tggccaagca gcgtggcgcg taccggggcc gcaagaaagc cctgtccgat gagcaggctg
    85861 ctaccctgcg gcagcgagcg acggccggcg agcccaaggc gcagcttgcc cgcgagttca
    85921 acatcagccg ggaaaccctc taccagtacc tccgcacgga cgactgacac atgccgcgtc
    85981 gcttgatcct ctcggccacg gagcgggaca ccctgcttgc gctgccggaa agccaggatg
    86041 acctgatccg ctactacacc ttcaacgact ccgacctgtc gctgatccgc cagcgacgcg
    86101 gcgacgccaa ccgcctcggc ttcgccgtgc agctctgcct gctgcgctac cccggttacg
    86161 cgctgggaac cgacagcgag ctgcccgagc cggtcatcct gtgggtggcg aagcaagtcc
    86221 aggccgagcc ggcgagctgg gcaaagtacg gcgagcgcga cgtgacccgt cgcgagcatg
    86281 cccaggaact gcgcacctac ctgcaactgg ccccgttcgg cctgtccgac ttccgcgccc
    86341 tggtgcgcga gctaaccgag ctggcccagc agaccgacaa aggcttgctg ctggccggtc
    86401 aggccctgga gagcctacgg cagaaacgac gcatcctgcc ggcgctgagc gtgattgacc
    86461 gggcctgctc ggaagccatt gcgcgagcca atcggcgggt ctaccgcgcc ctggtcgaac
    86521 cactcacgga ctcgcatcgg gccaagctgg acgagctgtt gaagctcaag gccggcagca
    86581 gcatcacctg gttgacctgg ctgcgccagg caccgctgaa acccaactct cggcacatgc
    86641 tggaacacat cgagcggctg aagacatttc agttggtgga cttgcccgaa ggcctgggcc
    86701 ggcacatcca ccagaaccgc ctgctcaagc tggcccgcga gggtgggcag atgacgccca
    86761 aagacctcgg taagttcgag ccgcagcgcc gctacgcgac cctggccgcc gtggtgctgg
    86821 agagcaccgc gaccgtgatc gatgagctgg tcgatctgca tgaccgcatc ctggtcaagc
    86881 tgttcagcgg cgcgaagcac aagcatcagc agcagttcca gaagcagggc aaggcgatca
    86941 acgacaaggt gcgcctgtac tccaggatcg gccaggcgct gctggaagcg aaggaaagcg
    87001 gcagcgaccc ctatgccgcc atcgaggcgg tgattccctg ggacgagttc accgagagcg
    87061 tcagcgaggc cgagctgctg gcccggccgg aaggcttcga ccacctgcac ctggtcggcg
    87121 agaacttcgc caccctgcgc cgttacacgc cggccttgct ggaggtgctg gaactgcgcg
    87181 ccgcgccggc cgcgcaaggc gtgctggcag ccgtgcagac cctgcgtgag atgaacgccg
    87241 acaacctgcg caaggtgccg gccgatgcac ccacggcctt catcaagccg cgctggaagc
    87301 cgctggtgat caccccggaa ggcctcgacc ggaaattcta cgaaatctgc gccctgtccg
    87361 agctgaagaa cgccctgcgc tccggcgaca tctgggtcaa gggctcgcgg cagttccgcg
    87421 acttcgacga ctacctgctg ccggccgaga agttcgccgc actcaagcgc gagcaggccc
    87481 tgcccctggc gatcaacccg aacagcgacc agtacctgga agagcgtttg cagctgctgg
    87541 acgagcagtt ggccaccgtc acccgcctgg ccaaggacaa cgagctgccc gatgccatcc
    87601 tcaccgagtc agggctgaaa atcaccccgc tggatgcggc ggtgccggat cgggcgcagg
    87661 cgctgatcga ccaaaccagc cagttactgc cgcgcatcaa gatcaccgaa ctgctgatgg
    87721 acgtggacga ctggacgggc ttcagccgcc acttcaccca cttgaaggac ggggccgagg
    87781 ccaaagacag gacgttgctg ctgtccgcaa tcctcggtga tgcgatcaac ctcgggctga
    87841 ccaagatggc cgagtcgagc cccggcctga cctacgccaa gctgtcctgg ctgcaagcct
    87901 ggcacatccg cgacgaaacc tattcggcgg ccttggccga gctggtcaac caccagtatc
    87961 gccacgcctt tgccgcccac tggggcgacg gcacgacctc atcctccgat ggccagcgct
    88021 tccgcgcggg tggccggggc gagagcaccg ggcacgtcaa cccgaagtac ggtagcgagc
    88081 cgggacggct gttctatacc catatctccg accagtacgc gccgttcagc acccgcgtgg
    88141 tgaatgtcgg cgtccgcgat tccacctatg tgctcgacgg cctgctgtac cacgagtccg
    88201 acctgcggat cgaggagcac tacaccgaca cggccggctt caccgatcac gtctttgccc
    88261 tgatgcacct gctaggcttc cgcttcgcgc cgcgcatccg cgacctcggc gaaaccaagc
    88321 tgtacgtgcc gcagggcgtg caagcctacc cgacgttgcg cccgctgatc ggcggcaccc
    88381 tgaaccacgg cagcctggcg gctgcaaatc gtggtgtggc agaatatgag ctttcagagt
    88441 aaccatccag aatgagttcc catgtcacac aacctggagc accagaaagt ccacaccaga
    88501 atggtgaaag aggttctgaa agccgtcgcc agggccaata atcaccctta tcagtctgta
    88561 tttacagact ttatcgccgg tcatccctcc tgtacggtct gtttctggga aaccttccac
    88621 aaaatgtacc cggattcacc gtacgaatac gtcacgttct gccatacctg ccgtcgtttt
    88681 gatttatacg aaactgaagc ggagatgaag gcggatgacc cgaagtggtg gtgattttca
    88741 gccccgaccg ctgaaacgcc tgttcacagc caaccagtgc tggacatcct tcctggatgc
    88801 gggcggcctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct gcggcacgcg
    88861 gatactgggt gtgaaggaat acaactgcga taaacctgag tgcccccacg tcagatacgt
    88921 cactaactca tgtggcagcc gtgcctgccc gtcctgcgga aaaaaggcca cagacctgtg
    88981 gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtgcacctgg tcttcaccct
    89041 gccggacacg ctgtggccgg tgttcgaaag caaccgctgg ctgctgaatg acgtgtgccg
    89101 tctggcggtg gagaatctgc tgtatgcagc ccggaaacgg ggtctggaac ccggtatctt
    89161 ctgcgccatc catacgtatg gccgtcgtct caactggcat ccgcatgtgc atgtgtcagt
    89221 gacctgcgga ggtctgaata aacacggaca ctggaaaaag ctgagcttcc tgaaagacgc
    89281 gatgcgttcg cggtggatgt ggaatatgcg gcagctgctt ctgaaagcgt ggtcagaagg
    89341 tctggcgatg ccggaatcgt tgtcacatat cacgacggaa tcacagtgga gaagcctggt
    89401 gctgaaagcc ggcggaaaat actggcatgt gtacatgtcg aagaagacag ccggagggcg
    89461 gaatacggca cgctacctgg gtcgttatct gaagaagcca ccaatagcgg cttcccggct
    89521 ggcgcattac aacggaggag cgagtctgag cttccgttac ctggaccaca aaacgggaga
    89581 aacggcgacg gaaacgctga cacagcggga gctggtcgcg aggctgaaac agcacatccc
    89641 ggagaagttt tttaagatgg tgagatactt cgggttcctt gccaaccggg tgtgtggaga
    89701 gaagctgccg caggtgtacc gtgctctggg gatggataag ccggaaccag tggcgaaagt
    89761 gtgctatgca caaatggtga aacagttcct gagtcgtgac ccgttcgaat gcgtgctgtg
    89821 tggcggccgg atggtatacc gccgggccat tgcggggctg aatgtatcag ggctgaagaa
    89881 aaacgcgcgg gatatcagtc tgctgaggta tatgccggcc tgacacaggc taagtgtgcc
    89941 cgggaatcgg tgcaggtata aaaaacagcc tgttacggta taaaaatcga ccatgcagag
    90001 agagaaacca ccggagtgga gggaaaagca gaaaggctca cgtggcataa ggcgtggaca
    90061 gcggtatcgt ctg
//
LOCUS       DAFATA010000006        89524 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFATA010000006 DAFATA010000000
VERSION     DAFATA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.006"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1533
                     /locus_tag="LGJ20_000657"
     CDS             <1..1533
                     /locus_tag="LGJ20_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2384777.1"
                     /translation="YYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEM
                     TELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYD
                     QESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYA
                     QCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGEN
                     AWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQ
                     PIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFR
                     HFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKETGLKANSLCVEITETVIERI
                     NEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKI
                     VRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLV
                     KE"
     gene            1541..2869
                     /locus_tag="LGJ20_000658"
     CDS             1541..2869
                     /locus_tag="LGJ20_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBK2384778.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2916..4241)
                     /gene="rimO"
                     /locus_tag="LGJ20_000659"
     CDS             complement(2916..4241)
                     /gene="rimO"
                     /locus_tag="LGJ20_000659"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBK2384779.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            4454..4837
                     /gene="bssR"
                     /locus_tag="LGJ20_000660"
     CDS             4454..4837
                     /gene="bssR"
                     /locus_tag="LGJ20_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBK2384780.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            4948..6063
                     /gene="yliI"
                     /locus_tag="LGJ20_000661"
     CDS             4948..6063
                     /gene="yliI"
                     /locus_tag="LGJ20_000661"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBK2384781.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6060..6686)
                     /gene="gstB"
                     /locus_tag="LGJ20_000662"
     CDS             complement(6060..6686)
                     /gene="gstB"
                     /locus_tag="LGJ20_000662"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBK2384782.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            6932..8134
                     /gene="dacC"
                     /locus_tag="LGJ20_000663"
     CDS             6932..8134
                     /gene="dacC"
                     /locus_tag="LGJ20_000663"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBK2384783.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8181..8939)
                     /gene="deoR"
                     /locus_tag="LGJ20_000664"
     CDS             complement(8181..8939)
                     /gene="deoR"
                     /locus_tag="LGJ20_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBK2384784.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(8997..9593)
                     /gene="ybjG"
                     /locus_tag="LGJ20_000665"
     CDS             complement(8997..9593)
                     /gene="ybjG"
                     /locus_tag="LGJ20_000665"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK2384785.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9878..11110
                     /gene="mdfA"
                     /locus_tag="LGJ20_000666"
     CDS             9878..11110
                     /gene="mdfA"
                     /locus_tag="LGJ20_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBK2384786.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11151..11435)
                     /locus_tag="LGJ20_000667"
     CDS             complement(11151..11435)
                     /locus_tag="LGJ20_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384787.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(11521..12336)
                     /gene="ybjI"
                     /locus_tag="LGJ20_000668"
     CDS             complement(11521..12336)
                     /gene="ybjI"
                     /locus_tag="LGJ20_000668"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBK2384788.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGCVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(12336..13544)
                     /locus_tag="LGJ20_000669"
     CDS             complement(12336..13544)
                     /locus_tag="LGJ20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2384789.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13628..14164
                     /gene="rcdA"
                     /locus_tag="LGJ20_000670"
     CDS             13628..14164
                     /gene="rcdA"
                     /locus_tag="LGJ20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBK2384790.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(14339..16024)
                     /locus_tag="LGJ20_000671"
     CDS             complement(14339..16024)
                     /locus_tag="LGJ20_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBK2384791.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHFGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16294..16671
                     /locus_tag="LGJ20_000672"
     CDS             16294..16671
                     /locus_tag="LGJ20_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBK2384792.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(16701..16958)
                     /gene="grxA"
                     /locus_tag="LGJ20_000673"
     CDS             complement(16701..16958)
                     /gene="grxA"
                     /locus_tag="LGJ20_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBK2384793.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17118..17405
                     /locus_tag="LGJ20_000674"
     CDS             17118..17405
                     /locus_tag="LGJ20_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBK2384794.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            17389..18111
                     /gene="nfsA"
                     /locus_tag="LGJ20_000675"
     CDS             17389..18111
                     /gene="nfsA"
                     /locus_tag="LGJ20_000675"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBK2384795.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            18172..19074
                     /gene="rimK"
                     /locus_tag="LGJ20_000676"
     CDS             18172..19074
                     /gene="rimK"
                     /locus_tag="LGJ20_000676"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBK2384796.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19162..19638
                     /locus_tag="LGJ20_000677"
     CDS             19162..19638
                     /locus_tag="LGJ20_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBK2384797.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            19990..21102
                     /gene="potF"
                     /locus_tag="LGJ20_000678"
     CDS             19990..21102
                     /gene="potF"
                     /locus_tag="LGJ20_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBK2384798.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            21197..22330
                     /gene="potG"
                     /locus_tag="LGJ20_000679"
     CDS             21197..22330
                     /gene="potG"
                     /locus_tag="LGJ20_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBK2384799.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIVSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            22340..23293
                     /gene="potH"
                     /locus_tag="LGJ20_000680"
     CDS             22340..23293
                     /gene="potH"
                     /locus_tag="LGJ20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBK2384800.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            23290..24135
                     /gene="potI"
                     /locus_tag="LGJ20_000681"
     CDS             23290..24135
                     /gene="potI"
                     /locus_tag="LGJ20_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBK2384801.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            24195..24683
                     /locus_tag="LGJ20_000682"
     CDS             24195..24683
                     /locus_tag="LGJ20_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBK2384802.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            24724..25851
                     /gene="rlmC"
                     /locus_tag="LGJ20_000683"
     CDS             24724..25851
                     /gene="rlmC"
                     /locus_tag="LGJ20_000683"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBK2384803.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(26026..26757)
                     /gene="artJ"
                     /locus_tag="LGJ20_000684"
     CDS             complement(26026..26757)
                     /gene="artJ"
                     /locus_tag="LGJ20_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBK2384804.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(27049..27717)
                     /gene="artM"
                     /locus_tag="LGJ20_000685"
     CDS             complement(27049..27717)
                     /gene="artM"
                     /locus_tag="LGJ20_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBK2384805.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(27717..28433)
                     /gene="artQ"
                     /locus_tag="LGJ20_000686"
     CDS             complement(27717..28433)
                     /gene="artQ"
                     /locus_tag="LGJ20_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBK2384806.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(28440..29171)
                     /gene="artJ"
                     /locus_tag="LGJ20_000687"
     CDS             complement(28440..29171)
                     /gene="artJ"
                     /locus_tag="LGJ20_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2384807.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(29189..29917)
                     /gene="artP"
                     /locus_tag="LGJ20_000688"
     CDS             complement(29189..29917)
                     /gene="artP"
                     /locus_tag="LGJ20_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBK2384808.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(30135..30650)
                     /locus_tag="LGJ20_000689"
     CDS             complement(30135..30650)
                     /locus_tag="LGJ20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2384809.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            30776..31099
                     /locus_tag="LGJ20_000690"
     CDS             30776..31099
                     /locus_tag="LGJ20_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK2384810.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            31096..31926
                     /gene="amiD"
                     /locus_tag="LGJ20_000691"
     CDS             31096..31926
                     /gene="amiD"
                     /locus_tag="LGJ20_000691"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBK2384811.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(31923..32936)
                     /locus_tag="LGJ20_000692"
     CDS             complement(31923..32936)
                     /locus_tag="LGJ20_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK2384812.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(33035..34465)
                     /locus_tag="LGJ20_000693"
     CDS             complement(33035..34465)
                     /locus_tag="LGJ20_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBK2384813.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(34476..35477)
                     /gene="ltaE"
                     /locus_tag="LGJ20_000694"
     CDS             complement(34476..35477)
                     /gene="ltaE"
                     /locus_tag="LGJ20_000694"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBK2384814.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(35514..37232)
                     /gene="poxB"
                     /locus_tag="LGJ20_000695"
     CDS             complement(35514..37232)
                     /gene="poxB"
                     /locus_tag="LGJ20_000695"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBK2384815.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPIVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(37365..38333)
                     /gene="hcr"
                     /locus_tag="LGJ20_000696"
     CDS             complement(37365..38333)
                     /gene="hcr"
                     /locus_tag="LGJ20_000696"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBK2384816.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPLADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(38345..39997)
                     /gene="hcp"
                     /locus_tag="LGJ20_000697"
                     /gene_synonym="priS"
     CDS             complement(38345..39997)
                     /gene="hcp"
                     /locus_tag="LGJ20_000697"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBK2384817.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(40141..41040)
                     /gene="lysO"
                     /locus_tag="LGJ20_000698"
     CDS             complement(40141..41040)
                     /gene="lysO"
                     /locus_tag="LGJ20_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBK2384818.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(41535..42230)
                     /gene="aqpZ"
                     /locus_tag="LGJ20_000699"
     CDS             complement(41535..42230)
                     /gene="aqpZ"
                     /locus_tag="LGJ20_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBK2384819.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTTDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            42656..44314
                     /locus_tag="LGJ20_000700"
     CDS             42656..44314
                     /locus_tag="LGJ20_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBK2384820.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(44311..45267)
                     /locus_tag="LGJ20_000701"
     CDS             complement(44311..45267)
                     /locus_tag="LGJ20_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBK2384821.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            45418..46533
                     /gene="macA"
                     /locus_tag="LGJ20_000702"
     CDS             45418..46533
                     /gene="macA"
                     /locus_tag="LGJ20_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBK2384822.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            46530..48476
                     /gene="macB"
                     /locus_tag="LGJ20_000703"
     CDS             46530..48476
                     /gene="macB"
                     /locus_tag="LGJ20_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBK2384823.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(48549..48773)
                     /gene="cspD"
                     /locus_tag="LGJ20_000704"
     CDS             complement(48549..48773)
                     /gene="cspD"
                     /locus_tag="LGJ20_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBK2384824.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            49178..50416
                     /locus_tag="LGJ20_000705"
     CDS             49178..50416
                     /locus_tag="LGJ20_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2384825.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHELNHTVQE
                     ALAPVTVKDALTYWLESYAKEKRTDHESLKSRINKHIISQIGAMPLDKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAVSNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIFPPYYSALIRLLIVFGCRTTELRRSEVREWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVAEYGRTAHRRINQAPWSL
                     HDIRHTFTTMLNDLGVDPHIVEQLTAHQMPGMQRVYNHSRYLDAKRDALNLWVDRLEL
                     LQNNDEKIVVMTPRIYTQNY"
     gene            50809..51018
                     /locus_tag="LGJ20_000706"
     CDS             50809..51018
                     /locus_tag="LGJ20_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBK2384826.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGSFPSRRNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAA"
     gene            51029..51970
                     /locus_tag="LGJ20_000707"
     CDS             51029..51970
                     /locus_tag="LGJ20_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBK2384827.1"
                     /translation="MTYITKATGGGRQWIHTKLERITNNATFAAGGQCDQSDLVRPKV
                     CNESFFLCSLRNFSRANEQGANLSDSCSIFLRRLFRAGDNVLVNSLSVIVLSCIAMRR
                     SYSHHGADSDYSDSLAFRRCSYFNLRPQASKTHAEKLALGLVNFSCSTLASIPSISSC
                     GNRIFFFADLLFVLPLDIDAPHLNGLYGRTQYSKKNEMLSIDMCAHMILICAHSLSFV
                     TQIKQRPAVLATHAGRLTTNDSKSIEVAMRNHTTHPQGRDSHNLNKYIWRFIALSTAQ
                     PRVITIEASSEQEARQQSPAGCVMVFAARIRQGVCHA"
     gene            51963..52283
                     /locus_tag="LGJ20_000708"
     CDS             51963..52283
                     /locus_tag="LGJ20_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384828.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEDYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            52290..52589
                     /locus_tag="LGJ20_000709"
     CDS             52290..52589
                     /locus_tag="LGJ20_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBK2384829.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFLSSLNVEIFICRFRREIPTSRNNHVSRVDVDKKLQELKNMIAAWREQK"
     gene            52586..54709
                     /locus_tag="LGJ20_000710"
     CDS             52586..54709
                     /locus_tag="LGJ20_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBK2384830.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLVELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERGELAEKKKVVELPVKKKEPCYQDDELKPRVESRVDGVF
                     WVTPKVDKQSGEIIRPETWLCSPLELLGTGTIGKEHYRVMRWKKLANHEVITMAIPCG
                     GIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGSHEEWQLSTTTGWHFGAYIMPD
                     GSIIGDSEKPILFTGKSAAVNGYSVAGTAEGWRDSVARLAGGNPSMMLGVATSLAAPL
                     IGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEAHND
                     GLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEMDVE
                     TFLKTEGIKVKAGQLVRLLNVPMEKATQFHEYSTGKAHADALKDAWTENHGAAGREWV
                     KWLADHQQEAKDTVRECRERWRNLIPDSYGEQVHRVGERFAILEAALVLSGHVTGWAA
                     QECRDAIQHNFNAWVKEFGTGNREFKQMVEQAEAFLSSFGFSRYLPYPNSDERDLPIK
                     DLAGYRKGSIRNEDDEFRFYTFPHVFEGEIAQGFNPSHFARALSAAGMLEAGNDRRYK
                     KKALGKIGGKQHVFYVMMFKPEAED"
     gene            54921..55343
                     /locus_tag="LGJ20_000711"
     CDS             54921..55343
                     /locus_tag="LGJ20_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK2384831.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMAMASMAVPLPCNQADDGQ
                     ATMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPSGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            55361..55609
                     /locus_tag="LGJ20_000712"
     CDS             55361..55609
                     /locus_tag="LGJ20_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384832.1"
                     /translation="MPVTFEEVQQHKKFHGIDDLETTTAKKYRRLLSSDALFVVDHHD
                     FLRSSLTGEIFATNREQVEAMIEYLWKIRRRMRDPVKQ"
     gene            55883..57052
                     /locus_tag="LGJ20_000713"
     CDS             55883..57052
                     /locus_tag="LGJ20_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK2384833.1"
                     /translation="MKKLIELRQQKNALKNQMRSLLEKADSENRSLNDDEGKQFDELR
                     AKADSLDTEISRLESVADEERSKPGTGIQKLSSDELRNYIVTGDVRSLSTSTDSGRDG
                     GYTVIPELDREVMRQLQDDSVMRVIATVKTAKSNEFQKLVSTGGATVGRGTEGSARSE
                     TNTPKIERVTIKLNPIYAYPKTTQEILDFSEVDILGWLSSEIADTFASTEEDDFVNGD
                     GNGKPKGFMAYTRAATSDKTRAFGTIEKMVAASGTAITADELIDILYKLKAKYRKNAV
                     WVMNSGTAGTLQKLKNENGDYIWRDSLKEGAPDMLLGRPVYCLESMPDIGAGKAPLAV
                     GDFSRGYFIVDHVTGIRTRPDNITEPGFYKVHTDKYLGGGVVDSNAIKILEMKAG"
     gene            57107..57667
                     /locus_tag="LGJ20_000714"
     CDS             57107..57667
                     /locus_tag="LGJ20_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBK2384834.1"
                     /translation="MKNTDFEIRTSELTASNKKLVGYAVRWNCLSEIIWDEFREQFTP
                     GAFADYLAAGNDVRCLYEHDYTRLLGRTKSGTLVLTEDNTGLRFELTPPDTQLGKDVL
                     TLVERGDITGMSFGFRALCEEWNIAQKPYLRTVTAAELREITITSMPAYPESGVEIAH
                     RSLFAQHPELCPTGNNRHRWAELAGL"
     gene            57669..58883
                     /locus_tag="LGJ20_000715"
     CDS             57669..58883
                     /locus_tag="LGJ20_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2384835.1"
                     /translation="MWWPFSRKKSEQRNLSIDDFLALSGVPNTGSGEYVSAGTAESLP
                     AVMNAVSVIAEAVATMPCYLYLVRNDKDREAREWLDSHPVDILLNEQPNSCQTPYQFK
                     RTMMRHCLLNGNAYAVIEWGRDGQPKSLHPYAPGCVVPERTGAHKYRYTITEPYTGTV
                     RTYLQEEVLHLRYASDDGFLGRSPVTICREALGLGLAQQRHGASIMKDGMMAAGIITS
                     GEWLDGVKGKQALDALERYKGAKNAGKTPILEGGMDYRQLGMSNQDAEWLASRRFSIE
                     DIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVP
                     GTRYLVEFDSADLLRATPTERYATYEKGIKNGIMNPNEAREREGMPPREGGDEFSQAW
                     KQTVEIKGRKDE"
     gene            58876..59178
                     /locus_tag="LGJ20_000716"
     CDS             58876..59178
                     /locus_tag="LGJ20_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK2384836.1"
                     /translation="MSEARITPDEVRAHLRLDDDLSGEGELLKMYTDAALEACQKHIG
                     KRFEDGLEFTPAMRVGCLMYIAFLYENREAVSPVEHSELPMAISALWSVYRDVGVY"
     gene            59178..59618
                     /locus_tag="LGJ20_000717"
     CDS             59178..59618
                     /locus_tag="LGJ20_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK2384837.1"
                     /translation="MPWQPLRRCTEPGCNKRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRALYLSKNPLCVRCLAKGIYTPALVVDHIIPINGGGDVLFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYREQEERAAHRNDWMYGDDD"
     gene            59602..59787
                     /locus_tag="LGJ20_000718"
     CDS             59602..59787
                     /locus_tag="LGJ20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384838.1"
                     /translation="MAMMTEQEQTRLIRGLIRQRDTWKTQETEHKANRTGRTKRTTAK
                     RLTDRDREVMECFRNRW"
     gene            59907..60263
                     /locus_tag="LGJ20_000719"
     CDS             59907..60263
                     /locus_tag="LGJ20_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBK2384839.1"
                     /translation="MARPPKAPAYLDEIAVRQWKEKSRQLSGREDLTPADWSNLELYC
                     VNYSIYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMIKMASLLGFDPVSRR
                     RNPPETEEEDELDCLA"
     gene            60247..61908
                     /locus_tag="LGJ20_000720"
     CDS             60247..61908
                     /locus_tag="LGJ20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK2384840.1"
                     /translation="MTAWHEYAEGVKNGKITACKRLKQAVKRYFSDLENPLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTVAAILANWFLVMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEMNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPWMGNGAWTECAGT
                     FAEADLYGQECYAGLDLSSTSDISSVCYAFPVGKKIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWVKAGWIRTTPGDCIDYDRIRDDIMADAENFNIRLVGFDTWNATHLRTQLQGAG
                     LEVEPFPQTYLRFSPAAKSFEVFVNRKVIVHRGDPVLAWSMSNVVMQSDANANIKPNK
                     KKSSNKIDPSVAALMAFGTFQAEHEEFAFDMSDSHKKRLAAFDGV"
     gene            61914..62195
                     /locus_tag="LGJ20_000721"
     CDS             61914..62195
                     /locus_tag="LGJ20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384841.1"
                     /translation="MTETDLLKIIRRITGISQQPDEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKKPERLRR"
     gene            63722..64042
                     /gene="clpS"
                     /locus_tag="LGJ20_000722"
     CDS             63722..64042
                     /gene="clpS"
                     /locus_tag="LGJ20_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBK2384842.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            64073..66349
                     /gene="clpA"
                     /locus_tag="LGJ20_000723"
     CDS             64073..66349
                     /gene="clpA"
                     /locus_tag="LGJ20_000723"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBK2384843.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(66693..66780)
                     /locus_tag="LGJ20_000724"
     tRNA            complement(66693..66780)
                     /locus_tag="LGJ20_000724"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66744..66746),aa:Ser,seq:gga)
     gene            complement(67034..67252)
                     /gene="infA"
                     /locus_tag="LGJ20_000725"
     CDS             complement(67034..67252)
                     /gene="infA"
                     /locus_tag="LGJ20_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK2384844.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(67537..68241)
                     /gene="aat"
                     /locus_tag="LGJ20_000726"
     CDS             complement(67537..68241)
                     /gene="aat"
                     /locus_tag="LGJ20_000726"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBK2384845.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(68283..70004)
                     /gene="cydC"
                     /locus_tag="LGJ20_000727"
     CDS             complement(68283..70004)
                     /gene="cydC"
                     /locus_tag="LGJ20_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBK2384846.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(70005..71771)
                     /gene="cydD"
                     /locus_tag="LGJ20_000728"
     CDS             complement(70005..71771)
                     /gene="cydD"
                     /locus_tag="LGJ20_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBK2384847.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(71894..72859)
                     /gene="trxB"
                     /locus_tag="LGJ20_000729"
     CDS             complement(71894..72859)
                     /gene="trxB"
                     /locus_tag="LGJ20_000729"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK2384848.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            73404..73898
                     /gene="lrp"
                     /locus_tag="LGJ20_000730"
     CDS             73404..73898
                     /gene="lrp"
                     /locus_tag="LGJ20_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBK2384849.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            74033..78139
                     /gene="ftsK"
                     /locus_tag="LGJ20_000731"
     CDS             74033..78139
                     /gene="ftsK"
                     /locus_tag="LGJ20_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK2384850.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGDFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            78294..78905
                     /gene="lolA"
                     /locus_tag="LGJ20_000732"
     CDS             78294..78905
                     /gene="lolA"
                     /locus_tag="LGJ20_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBK2384851.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            78916..80259
                     /gene="rarA"
                     /locus_tag="LGJ20_000733"
                     /gene_synonym="mgsA"
     CDS             78916..80259
                     /gene="rarA"
                     /locus_tag="LGJ20_000733"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBK2384852.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            80350..81642
                     /gene="serS"
                     /locus_tag="LGJ20_000734"
     CDS             80350..81642
                     /gene="serS"
                     /locus_tag="LGJ20_000734"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK2384853.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            81881..84325
                     /gene="dmsA"
                     /locus_tag="LGJ20_000735"
     CDS             81881..84325
                     /gene="dmsA"
                     /locus_tag="LGJ20_000735"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBK2384854.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            84336..84953
                     /gene="dmsB"
                     /locus_tag="LGJ20_000736"
     CDS             84336..84953
                     /gene="dmsB"
                     /locus_tag="LGJ20_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBK2384855.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            84955..85818
                     /gene="dmsC"
                     /locus_tag="LGJ20_000737"
     CDS             84955..85818
                     /gene="dmsC"
                     /locus_tag="LGJ20_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBK2384856.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(85854..86480)
                     /locus_tag="LGJ20_000738"
     CDS             complement(85854..86480)
                     /locus_tag="LGJ20_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK2384857.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            86794..87942
                     /locus_tag="LGJ20_000739"
     CDS             86794..87942
                     /locus_tag="LGJ20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2384858.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            88152..>89524
                     /locus_tag="LGJ20_000740"
     CDS             88152..>89524
                     /locus_tag="LGJ20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2384859.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFV"
BASE COUNT        22315 a        21908 c        23743 g        21558 t
ORIGIN      
        1 tattatcctg gcggtaattt attgaacgcc tcagataaac ttttttatct gccgtttgcg
       61 ttcattatta tcgtattgct ggtggtttat ttaatgacca ctcgtgtgtt ccgtcggcaa
      121 ttttctgaaa tgacagagct ggttaatacg ctggcatttt tgcccgactc aacggatcaa
      181 atcgaggctc tgaaaattcg cgaaggcgat gcgaaagaga ttatcagcat taaaaattcg
      241 atcgcggaaa tgaaagatgc cgaaattgaa cggtcaaata aattgctctc actgatctct
      301 tacgatcagg aaagcggttt tattaaaaat atggcgatta ttgagtccaa caataatcag
      361 tatctggctg tggggatcat caaactgtgt ggtctggaag ccgtggaagc ggtgtttggt
      421 gttgatgaac gcaataaaat cgtcaggaaa ttgtgtcagc gaattgccga gaaatatgcg
      481 caatgctgcg atatcgtgac attcaatgcc gatctctatt tacttctgtg tcgggaaaat
      541 gtacagacat ttacccgtaa aatagcgatg gtaaacgatt ttgacagcag ctttggctac
      601 cgcaatctgc gcatccataa gtctgccatt tgtgaacctt tgcaggggga aaacgcctgg
      661 agttacgcag aaaaactgaa actggcgatt tccagtatcc gtgaccatat gttctccgag
      721 tttattttct gtgatgacgc gaaactcaac gaaatagaag agaatatctg gattgcgcgt
      781 aatattcgcc atgcaatgga aattggcgaa ctattcctcg tctatcaacc gatcgttgat
      841 attaacaccc gcgccattct gggcgcggag gcgttgtgcc gttgggtttc tgcggagcgg
      901 gggatcattt caccgctgaa gttcattacc attgctgaag atatcgggtt tatcaatgag
      961 ctgggttatc agattattaa aacggcaatg ggtgaattca gacattttag tcagcgtgcg
     1021 tcgctgaagg atgatttctt actgcatatt aatgtttcgc cctggcagtt aaacgaaccg
     1081 cactttcatg agcgttttac caccatcatg aaagaaactg gcctgaaggc gaacagcctc
     1141 tgtgttgaga tcactgaaac cgtgattgag cgaattaatg aacattttta tctcaatatt
     1201 gaacaactgc gtaaacaagg ggtacggata tcgattgatg actttggcac cggtttgtca
     1261 aacctgaaac gtttttatga aattaatcca gacagcataa aggtggactc gcaatttacc
     1321 ggcgatattt tcggtactgc gggaaaaatt gtgcgcatta ttttcgatct ggcacgctat
     1381 aaccggatcc cggtgattgc ggaaggcgta gagagcgaag acgttgcgcg cgaattaatt
     1441 aaattaggat gtgttcaggc tcaggggtat ctgtaccaga aacccatgcc attctccgcc
     1501 tgggataaaa gtggaaaatt agtaaaagag tagtttacgt atgtccagaa tcaataagtt
     1561 cgtacttaca gtcagtctgc tgatttttat catgatttca gcagttgcct gcgggatcta
     1621 cactcaaatg gtaaaggaac gggtgtatag cctgaaacag tccgttattg atactgcttt
     1681 tgcggtggca aatattgctg aatatcggcg tagcgtggca attgatctta tcaacacgct
     1741 aaatcccacg gaggaacagc tgttggttgg tttgcgcaca gcttacgccg actcggtttc
     1801 cccctcttat ttgtacgatg tcggtcctta tctgatttcc agtgacgaat gtattcaggt
     1861 aaaggagttc gagaaaaatt attgtgcaga tattatgcag gttgtgaagt atcgacatgt
     1921 caaaaataca gggtttatct cttttgacgg taaaaccttc gtctattacc tctatccggt
     1981 aactcacaat cgtagtctga tatttttgct tggtctggag cgtttttctt tactgtcaaa
     2041 atcgctggcg atggacagcg agaacctgat gttctctcta tttaagaacg gtaaaccggt
     2101 gaccggtgat gaatataatg ctaaaaacgc catcttcacc gtttcggaag cgatggagca
     2161 cttcgcctat ttgccgaccg gattgtacgt atttgcgtat aaaaaagatg tttatttgcg
     2221 ggtctgtaca ttgattattt tctttgccgc attggtggca gtgatatcgg gtaccagttg
     2281 cctctatctg gtacgcagag tgattaatcg tggtattgtg gagaaagaag ccatcattaa
     2341 taaccatttt gaacgcgtac tggatggcgg gcttttcttt tcggctgccg atgtcaaaaa
     2401 actctacagt atgtataact cggcgttcct ggacgacctg accaaagcaa tgggcagaaa
     2461 atcctttgac gaagatttaa aagcgctgcc ggaaaaaggc ggttatttgt gcctgtttga
     2521 cgtcgataaa ttcaaaaaca ttaacgacac cttcggtcat ttgctgggcg atgaagtgtt
     2581 gatgaaagtg gtgaaaatcc ttaaatcaca gatcccggta gataaaggta aaatctaccg
     2641 cttcggcggt gacgaatttg cggtgattta tacgggtgga acgctggaag agttgctatc
     2701 gattctaaaa gaaatcgttc atttccaggt gggaagcatt aatttaagta ccagtatcgg
     2761 tgtagcgcat tcaaatgaat gtaccaccgt cgaacgcttg aaaatgctgg cggatgagcg
     2821 gctgtataag agtaagaaaa acggcagggc tcagataagc tggcagtaat cattattcgc
     2881 aggccggaca aatgattttg cccggcctga attaattaaa cccggctacc ccacaaatcg
     2941 tactcatcgg cgtgctcgac tttcacacgc aggatatcac ccggcttaac gttggtttca
     3001 ccattgagat aaaccgcgcc gtcgatttcc ggtgcatctg ccatgctgcg accaatcgcg
     3061 ccttcttcgt ccacttcgtc gataatcacc agaatttcac ggcccacttt ctcttgcagg
     3121 cgctcggcgg aaatctgctg ctgcagctgc atgaaacggt tccagcgttc ttctttaact
     3181 tcttccggaa cctggtcagg cagggcattg gcgtctgcac cttcaaccgg gctgtattta
     3241 aagcagccaa cgcgatccag acgcgcttct ttcaggaagt cgagtagcat ctggaaatct
     3301 tcttctgtct cgccagggaa gccgacaata aaggttgagc gtagggtcag ttccgggcag
     3361 atttcgcgcc actgtttgat gcgcgccagt tggcgatcta cagaacccgg acgcttcatc
     3421 agtttgagaa tgcgcgggct ggcgtgctgc aacggaatgt ccagatacgg caggattttg
     3481 ccttctgcca tcagtgggat gacgtcgtcc acatgcggat aagggtaaac gtagtgcaga
     3541 cgtgtccaga tccccagttt cgataactgt tcgcacaggc tgaccatgct ggtttttacc
     3601 ggctcgccgt tgtggaagcc agtacgatgt ttaacatcaa cgccataggc ggaagtatcc
     3661 tgcgagatca ccagaatctc tttaacgccc gcatctacca gacgtttcgc ttcacttaac
     3721 acttcgccaa tcggacggct caccaggtcg ccgcgcatag acggaataat gcagaaggtg
     3781 cagcggtgat tacagccttc cgaaattttc agataggcat aatgacgcgg cgtcagtttc
     3841 acaccttgtt ctggcaccag gctcaggaat gggttgtgtt tcggttttgg cacgtagtga
     3901 tgaacgtgct ccagaacctg ctcatagcta tgaggcccgg tgatttccag cactttcggg
     3961 tggacttcgc ggatctgatc ttcttttgcc cccagacaac cggtcacaat taccttgccg
     4021 ttttcattca acgcttcacc aatggcttcc agtgattctt gtaccgcgct gtcaataaag
     4081 ccgcaggtgt tgacgatcac catgtccgca tcgtcatagc tcggtaccac gtcataacct
     4141 tcagtgcgga gttcggtgag aatacgctct gaatcaacaa ggttttttgg acagccaagg
     4201 gaaacaaagc cgattttcgg ctggggagtt actttgctca tagcttaaaa aatattcagt
     4261 tacaggaaag gtcagggcag ggattctaca gagttctgca taaaatttgt atcgcaatct
     4321 cattcgctgg cggaggcgaa ggaaatgtaa attttgttaa ttcggcgtga agaattgatc
     4381 ctggacagca ttttgctcaa aaaatagcca tactatttaa ttgcaacaag gctggaaaga
     4441 ggaggatcga agtatgttcg ttgacagaca gcgaatcgat ctgctgaacc ggttgatcga
     4501 cgcacgcgtt gacctcgccg catacgtgca actgaggaag gcaaaaggat acatgtccgt
     4561 cagcgaaagc aatcatctac gagataactt ttttaaactg aatcgcgaac tgcacgataa
     4621 atcgctgcgg ttgaatcttc atctggatca ggaagagtgg agtgctcttc atcatgctga
     4681 agaagcatta gcgacagccg cagtatgttt gatgagtggg caccatgatt gcccgactgt
     4741 tattaccgtc aacgccgata agcttgaaaa ttgtctgatg agcttaacgc tgagtatcca
     4801 gagcctgcag aagcacgcca tgcttgagaa ggcctgaaaa ctaaggggga gaaagcgtct
     4861 cccccttcat gtttaagttt ttgtaaaaat gaatttgtta tctcctccac tgactacgct
     4921 ttaagccaga gtcaatccgg aggcgttatg catcgacaat cctttttcct tgtgcccctt
     4981 atttgtcttt cttccgctct ctgggcggct cctgcaacgg taaatgtcga agtactgcaa
     5041 gacaaactcg accatccctg ggcactggcc tttttacccg ataatcacgg tatgttaatc
     5101 actctgcgcg gcggcgagtt gcgtcactgg caagcaggaa aaggattatc tgcgccgctt
     5161 tccggagttc cggacgtttg ggcgcacggg cagggcggcc tgctggacgt ggttttagcg
     5221 cctgattttg ctcagtctcg ccgcatctgg ttaagttatt ccgaagttgg cgatgatggc
     5281 aaagccggaa ctgctgtggg ttatggccgc ttaagtgatg atctctcaaa agtgaccgac
     5341 ttccgcaccg tcttccgcca gatgccaaaa ctgtctaccg gcaaccattt tggcgggcgg
     5401 ctggtattcg acggtaaagg ttatcttttt attgctctgg gcgaaaacaa tcagcgcccg
     5461 acggcgcagg atctggataa attacagggc aaactggtgc gtctgaccga ccagggcgaa
     5521 atcccggatg ataatccttt tataaaggaa tccggtgtgc gcgccgagat ctggtcttat
     5581 ggcattcgta atccgcaagg aatggcgatg aatccgtgga gtaatgcact gtggctgaat
     5641 gaacatggcc cgcgcggtgg tgatgaaatt aatatcccgc aaaaaggcaa aaactacggc
     5701 tggccgctgg caacctgggg aatcaactat tcaggcttta agataccgga agcgaaaggg
     5761 gagatcgtcg ccgggaccga gcaacctgtt ttttactgga aagattcgcc cgctgtgagc
     5821 ggcatggcct tctataacag cgataaattc ccccagtggc agcaaaaatt atttattggc
     5881 gcgctgaaag ataaagatgt cattgtgatg agcgtcaacg gcgacaaagt gacagaagat
     5941 ggccgtattt taacggacag agggcagcga attcgtgatg ttcgcactgg acccgacggt
     6001 tatttatacg ttctcaccga cgagtccagt ggggaattac ttaaagttag cccacgcaat
     6061 tagctaacgg gaatcatcac cactttgcgc actgcggggc gttcagtgag ttgctgatac
     6121 cagcgttgca gatttggacg cggtgtccag gtcaggccaa cgttaaacaa attgtagatg
     6181 aacggtgcga tagcgatatc gcccacacca aactcgtcgc cagagaacca ttttactttt
     6241 gccagttccg catcgagcag ggcaaacagg gcgtcgcact ctttgcaact ggcatcaatg
     6301 gcggcctgat cgcgctcttc tggtggtgtt ctgactaatc ccatcaggat cccgcgatga
     6361 gcattactga gcgtctggtt tgcccagtcc atccattttt ccgcttccgc acgacgtgcc
     6421 ggtgagtcga tccacagacg tttttgcccg tactgtgccg ccagatagcg gacaatggcg
     6481 tttgattccc aaagaatgag atcactttcg tcgtcacgca acaacggcac cagcccgtta
     6541 gggttcatcg ccagaaaatc agcatcgtga tttatcccaa actcacggcc cgcgagaatt
     6601 tgctcataag gtagttccag ttcttcgagc gtcagcaata ctttttttac gttagttgaa
     6661 ttattccgac cccacagcgt aatcatactc accccttttc cgacaggcag ccacaggctg
     6721 aacaggactc cgatggtgaa gtaaagttaa cttttaagca acagctggca aaaaaatgac
     6781 accagaagca gcgaagcggc aggatattgc ataaacttta aaaactttac caacttacgg
     6841 tttctttaag tttgtgtgtg cgttattaat caccgaactt atcatacggc gatataacgt
     6901 atttttttga atggatactc gggtggcatt tatgacgcaa tactcctctc tccttcgtgg
     6961 tcttgcagcg ggttctgcat ttttattcct ttttgcccca acggcattcg cggcggaaca
     7021 aaccgttgaa gcgccgagcg tggatgcgcg tgcatggatt ttaatggatt acgccagcgg
     7081 taaagtgctg gcagaaggca acgcggatga gaaactggat cccgcgagcc tgactaaaat
     7141 catgaccagc tatgtggttg ggcaggcgct taaggccgat aagattaaac tcaccgatat
     7201 ggtgacggtc ggtaaagatg cctgggcgac gggaaatccg gcactgcgtg gttcatcggt
     7261 aatgttcctc aaaccgggcg atcaggtttc ggtggcagac ttgaacaaag gtgtgattat
     7321 ccagtccggt aatgacgcct gtattgcgct ggccgattac gttgccggga gccaggagtc
     7381 atttattggt ttgatgaatg gttatgccaa aaaactgggt ctgaccaaca ctaccttcca
     7441 gacggtgcac ggtctggatg cgccggggca gttcagtacc gcgcgcgata tggcattgct
     7501 gggtaaagca ttgatccacg atgtgccgga agagtacgcc attcataaag agaaagagtt
     7561 caccttcaac aaaattcgtc agcctaaccg taaccgtctg ctgtggagca gcaatctgaa
     7621 tgttgatggc atgaagacag gaaccacggc aggcgcggga tataatctgg ttgcttcggc
     7681 tacccagggc gatatgcgtt taatctccgt agtgctgggg gcgaaaactg accgtatccg
     7741 ttttaatgag tctgagaaat tattgacctg gggtttccgc ttctttgaaa ccgtgacgcc
     7801 aattaaacct gatgccacct ttgtgactca gcgcgtctgg tttggtgata agagcgaagt
     7861 gaatcttggg gcaggtgaag cgggctcagt gaccataccg cgtgggcagc tgaaaaacct
     7921 gaaggcgagc tacacgttaa cggaaccgca gcttaccgca ccgctgaaaa aaggtcaggt
     7981 tgtcgggacc attgatttcc agcttaacgg taaatccatt gagcagcgtc cgctgatcgt
     8041 gatggaaaat gtggaagagg gcggattctt tggtcggatg tgggatttcg tgatgatgaa
     8101 attccatcag tggttcggca gctggttctc ttaatcttct gataaccgga tggcgcgaaa
     8161 cgtcatccgg ttatacgtca ttaatacatc aacttaatac ggtgcgtctg cgcgtacttc
     8221 acatactcat cttccgggca acaatcactc accacaatat caaagcgttt caggtcaccc
     8281 atgcgcgccg gacgcacctt gccaaattta ctgtggtcga caaccagcac atgcttttgc
     8341 gccatcgaca tggcccagtg ttttaccggc aactcttcaa gattaaaaca ggtagcgcct
     8401 ttactgacat gcacgcccgc cgcggaataa aaagcgatat ccgggcaaaa attattcaac
     8461 gtttgctgaa aatcgatggg tttgaaaatg gcgttgctgg cgtgaaattc accaccgcac
     8521 agaaatgcgc ggcaatgggg tttctctttc agcgccagaa aggtatttag cgaataacaa
     8581 acggcggtaa aagggatttc attatcaatc gcttcaataa tccacggcgt ggtggtgcca
     8641 cagtcaaaaa agagagtctg atcgggttct accagtgtcg cagccatttt tgccgcccgg
     8701 cgtttttctt ccaccaggcg ggatttttga tcgcttaaca ggtaatggct ggcactgcgc
     8761 ggttccagaa caatatagcc gccgagcaaa acgacgggcg cactgtggtt gttcagatcg
     8821 cgacgaatcg tcatctccga aaccccaagc agggcggcgg cgtctttaag atgtaactta
     8881 tcgctgcgtt ttaattcttg cagcagctgc ccgatacgct cttcgcgacg tgtttccata
     8941 atccctctga atagttattg aagcgagccg ctcaatacta cactttttag cagagatcag
     9001 tcacgcaccc agcctttgcg gatcggtaat gcaaaacaga agcgatacca cgattgcaga
     9061 cgttgataga gtttatgccc catcgcttgc cagataatct gagcgctaag gcagccaatc
     9121 atacctgcca gcaatccacc gagcatatcc agcggccagt gcacgccaag ataaacgcgc
     9181 gaccaggcaa tgacgacggc cagcaccatt aaaagtgagc cggaccacag gcgatgccag
     9241 cataaaaatg ccagtgcaaa ggtgaaaatc accgtaccgt ggtcgcttgg gaatgagtca
     9301 tccgccgcat gatgcaggaa gttatagccg atattttcga caaagggtcg gtcgtgcgga
     9361 aaaagatgtc ccatcgtcca ggacacaaac aggctgaccg ccagcgcgat agcgattttt
     9421 atcaccagtt gccgttgtgc tgtaagcccc cacaaccaaa gcaccgcggc cagcagcggt
     9481 accacggtaa tcaaatcttt agcaataaaa atcgccaacg agatcatcca cggagccgag
     9541 tctggcgtcg cgttaataag agagaataga gagagattca aattttccag cataacttcc
     9601 cgacgcaaag tgattaaaag ggagccaata caggcaagtc gttgagaata aagtgcaggt
     9661 taaactgggt aaagcggcat cgtcttattt ccctcaagcg gcctgtttac ggtgggtaat
     9721 tgtaacgggc ataggttaaa taaaacttaa agaaagcgta gctatactcg caataatgta
     9781 agaatgtgct taaccgtggt ttcagctaca aaattcgctt tctcgttagc tgtgctttta
     9841 ttaaactctg cgcgattatt attggcgaag aaattgcatg caaaataaat tagcttccgg
     9901 tgccaggctt ggacgtcagg cgttactttt ccctctctgt ctggtgcttt acgaattttc
     9961 aacctatatc ggcaacgata tgattcaacc cggtatgttg gccgtggtgg aacaatatca
    10021 ggcgggcatt gattgggttc ctacttcaat gaccgcatat ctggcgggcg ggatgttttt
    10081 acaatggctc ctggggccgc tgtcggatcg tattggtcgc cgtccggtga tgctggcggg
    10141 ggtggtgtgg tttatcatca cctgtctggc aatattactg gcgcaaaaca ttgaacaatt
    10201 cactctgttg cgcttcttgc agggcataag cctctgtttc attggcgctg tgggatacgc
    10261 cgcaattcag gaatccttcg aagaggcggt ttgtatcaag atcaccgcgc tgatggcgaa
    10321 cgtggcgctg attgctccgc tacttggtcc gctggtgggc gcggcgtgga tccatgtgct
    10381 gccctgggaa gggatgtttg tcttgtttgc cgcattggca gcgatctcct ttttcggtct
    10441 gcaacaagcc atgcctgaaa ccgccacgcg tataggcgag aaactgtcac tgaaagaact
    10501 cggtcgtgac tataagctgg tgctgaagaa cggccgcttt gtggcggggg cgctggcgct
    10561 gggattcgtt agcctgccat tgctggcgtg gatcgcccag tcgccgatta tcatcattac
    10621 cggcgagcag ttgagcagct atgaatatgg cttgctgcaa gtgcctattt tcggggcgtt
    10681 aattgcgggt aacttgctgt tagcgcgtct gacctcgcgc cgcaccgtac gttcgctgat
    10741 tattatgggc ggctggccga ttattattgg tctgttggtc gctgctgcgg caacggttat
    10801 ctcatcgcac gcgtatttat ggatgaccgc cgggttaagt atttatgctt tcggcattgg
    10861 tctggcgaat gcgggactgg tgcgattaac cctgtttgcc agcgatatga gtaaaggtac
    10921 ggtttctgcg gcgatgggaa tgctgcaaat gctgatcttt accgtcggta ttgaaatcag
    10981 caaacatgcc tggctgaacg ggggcaacgg actgtttaat ctcttcaacc ttgtcaacgg
    11041 aattttgtgg ctgtcgctga tggttatctt tttaaaagat aaacagatgg gaaattctca
    11101 cgaagggtaa aaaaatgcct gattgctttg tgcgatcagg cattctcgaa ttaatggtga
    11161 tggtcgtcaa tctggtgttc gataaccatc ccttcaccta cgctggcaag atggcgaaca
    11221 taaggatgcg ggcggtaagc cggagctggc gcaggagcca catagacggt ttgcggcaca
    11281 gtcgcgacgc tgaccgcttg tggaacgctg actgaatcag gaaccacaac cactttgtat
    11341 ccactcggca catcgacggt gacactttgc gccatcgcca caccagtaaa gcccattaaa
    11401 agcgcgccca acagtagaca atttttcata aacatttcgc ctgaaataat gcagcgggtt
    11461 aatttccgcg aattatgcag ccttcattca ggtattgtgt tgccgggcgt gctgccgttt
    11521 ttattggtca aatggcgctt cgtgtttaag cactttatcg atcacatcca gtacgccttc
    11581 acggttattg gagcctgccc ggtattttgc cgctgcgacg accgcattgc cggcattttc
    11641 cattgcaaaa ctaaagcctg cctgacgcag catctcaata tcgttaccgc catcgccaaa
    11701 gaccaccact tcgctgtcgt ctattcccca taatttctgc agttggcgaa ggccattggc
    11761 tttatgtacg ccggggataa tcagatcgat gctgccgttg ccggtgtgga ccggcaccat
    11821 aatatcgccg atggcctcat gtaatgcttt ttgtacttgt ggaatcagtt catcggaaag
    11881 attcaggcca aacttaaaga agatatcctc taagttgtca aagttatcga cgtattccag
    11941 acggtgataa tacatttccg ccaccgtttt catggcatcg tcatattttt tgagcgtata
    12001 ggcgctattt tttccgcagg caataatttc cacttccggg cgcgtcagca aatgttccac
    12061 gacagtagta aacgcatcct tcgacagctc gccattaaaa acatctttgc cttcgctcac
    12121 tacacagccg ccgttttcag ccacaaaggc aatttcatta gcaatttcag ggaagaaaga
    12181 gatcaactga taatattgat tcccgctggc gactacaaag cgaattcctt gtgctttcat
    12241 ttgctgatac tgagccataa accgctcacg gttataggtt ttttgatcgc ttaagaaagt
    12301 accatccatg tctaccgcaa ttaatttaat gctcatcaac tattctccat cgccgtctgc
    12361 gttttggtat cgggtttggc gacggctttc gcgacaatag ccgcgagaat aaccagcgcc
    12421 agtacaacca gcattgcact acgtaatcca taatgttcgc cgagatagcc cagcagcggc
    12481 ggcccgacga ggaaagccag ataaccggtc gttgctacca cactgacgcg ggttggtgca
    12541 tcggggccgg tatcgctggc ggcagaaatg gtcagcggga agcccagcga ggcacccagt
    12601 ccccagagta caacagacac cccagcgacc caggcgctat cgacaaaaat aatcagccca
    12661 atacccaacg cccccattag tgcactggcc cgaaccacgg caacgcgact gtaacggtcg
    12721 atgaaccaac cgccggtgaa gcgtccaacg gtcatcccca gggtaaaacc ggcataaatc
    12781 agcgagccgg aagtagggct aaaaccgtga ccgtcaacca ttaataaggg taaccagtcg
    12841 ttggcagaac cttcggcaaa ggccatcgcc agcaccacaa cacctatcag cagcaactgg
    12901 atatcgcgat aaaaaggaat gcctttttcg ccatgctggg tgccatcggc agcatttttg
    12961 cccgtaccgt cagggattgc ctgaatggcg atataaatag gcgcgatacc taccagcgcc
    13021 gccaataaaa tgtgcaccgt tgccggaacg ccaaaggccg tcagtgccat cccgacacca
    13081 gcgcctgcca gcgtgcccag gctataaaaa ccgtgcatca ttggcaaaac cgttttattc
    13141 atttctcgct caacggcggc accttcaacg tttatcgcca cttccgcaga accaaaactt
    13201 gccccaaaga cgccgagacc aacggcaaag agcaggggcg atgtcagcca gagtgccaga
    13261 cttaatatca tcatcccgat caatgcgcag gacatcgtga ccaggatgac attacgcgtg
    13321 ccaaagcgtt tcactaacca cgccgagcag agaataccgc tcatcgaacc gatcgacaga
    13381 ccaaagagaa caccgcccat ttcagcgatc gagacagaga gaatatcgcg gatagcaggc
    13441 gtacgggttg cccaggacgc cattaacagg cctggcaaaa agaagaacat aaacagcgcc
    13501 caggttcggc gtttcaatgc attacgtgaa gaatttacgg tcatagatca cgtcaaaata
    13561 agaagaggga agacaacatt agcaaggttg tgtacatttg tacacaattg cagagagagg
    13621 aaatgacatg cgtcgcgcta acgatccgca acggcgagaa aaaattatcc aggccacgct
    13681 ggaggcggtg aaactttacg gaatacatgc tgttacgcac cgcaaaattg ctacccttgc
    13741 cggggtaccg ttgggatcga tgacctacta tttttcagga attgatgagt tgttgctgga
    13801 ggcgttcagc agttttactg agattatgtc ccggcaatat caggcatttt ttagcgatgt
    13861 tagtgatgct caaggcgcat gccaggctat caccgatatg atctacagct cacaggttgc
    13921 aacgccggat aatatggagc tgatgtacca gctctacgcg ctggctagcc gaaaaccgct
    13981 attaaaaacg gtaatgcaaa actggatgca gcgcagtcag caaacgctcg aacaatggtt
    14041 tgaacccgga accgcccgcg cgcttgatgc gtttattgag gggatgacgc tgcattttgt
    14101 caccgaccgt aagccgctat cgcgcgagga gattttgagg atggttgaga gggttgcagg
    14161 gtagtagata aattttagat aacaaaaaac ccatcaacct tgaaccgaaa tggcggggtt
    14221 gatgggctcc acaaaatggg gacatcaaag aaaagcagtg gcactaatta agactgatgc
    14281 cctgcggaaa agttctgcgg ttgtgcaaaa aaatttcatt ttcagggcaa cttcagtttt
    14341 atcctaatcc tggccatacc atgacgatga ttgtccctgc cagcgtcagc aggacgttgg
    14401 cgattgcgta ggtgcccgca tagcccagcg cagggatgtt actgcgagct gtatcactga
    14461 tgatctccat tgccggcgcg caggtgcgtg cgcccatcat tgcgccgaac aacagtgcgc
    14521 ggttcattcg caatacataa gcaccgaaca agaaacagat aaccacgggc acaagactga
    14581 caattaatcc ggcaatcaac atctgaccgc caatcgcgcc caggccgtta ttaataccgc
    14641 taccggcgct cagaccaacg cctgccataa acaccatcaa gccgaactct ttcaccatgc
    14701 ttaatgcacc ttgcggaatg taaccgaagg tcgggtggtt agcacgcata aagcccagca
    14761 taattccggc gaataacaac ccggcagcgt tccccatgcc gaaactgaat gtgctgaact
    14821 ggaaggtgat catcccgatc atcagcccaa taacaaagaa ggcgcaaaat gccagcaggt
    14881 cagtgacctg gctgtgaatc gagataaagc cgatgcgatc ggcgatggtt tttacgcgac
    14941 gggcatcacc gctgacttgt aaaacgtcac ctttgttaag cacgacgttg tcatctatcg
    15001 gcatctcaat ctggctacga atgacgcggt taaggaagca accgtgatcg gtcaacttca
    15061 gttgtgcgag acgtttacct acagcgttat ggtttttaac gaccacttct tcagtgacga
    15121 tacgcatgtc gagaaggtca cgatcgaaaa cttctttacc gttacggaag ctgggatcga
    15181 gtcgggcatg ggcgtcggga tagcctacca acgctatttc atcgcccatt tgtagcacgg
    15241 catcaccgtc tggatttgcc agaatcccgt tacgtcgaat acgttcaatg tagcagccgg
    15301 tttgtcgata aatacccagt tcacgcagat ttttgccgtc ggtccaggcc accagctccg
    15361 ggccgacgcg ataggcgcgg atcaccggta aataaacctt acggttggca tcagtgtcca
    15421 ggccacgttc gcgggcgatt tgctgggcgc tggtctgtaa gtcctgatgc tgcaatttcg
    15481 gcaagtaacg cgcaccaaca atcaaactca ccagaccgat taaataggtt aaggcatacc
    15541 cgaggctcag attatccagt gccagtgaga gctgcctgct ttccatgccg aaatgacgca
    15601 gtgtatcgcc agcaccgacc agaaccggtg tcgacgtcat agagcctgct aacataccgg
    15661 ccgtcaggcc aatatcccag ccaaacagct tacctaaccc taaggcgatc accagcgcac
    15721 tgccaaccat caccagtgct aacattaggt aatttttccc atcgcgaaaa aaaatggaaa
    15781 aaaagttcgg tccggcttcg accccgacgc agaaaataaa cagcataaag ccaagattaa
    15841 gcgcatcggt gttaatgctg aaatgttgtt ggcctaataa cagcgatacg actaaaacgc
    15901 caatggaatt acccagttgg atcgaaccaa gtcgtaactt tccgagacat agcccaagcg
    15961 cgaggaccac aaataataac agaatgtaat tcccatttaa caattcggcg acgtttatat
    16021 tcacggaggc taacttcttg tttactagta agctgttgaa agaaatggta atttacgata
    16081 atgtttttta ccagaattca gggcgcagat tcattcagcg cacctaaacg atagtaaagt
    16141 aacaatatat tttactagtg taatcacatt aggtatcaac ggctatatga attgcgttgg
    16201 cctatattag catggaatgc gaagcggctt tatcttactg aacgccacac tggcgaaaaa
    16261 tgtgttcgat agacgcagtg tcaggaggaa cgagtgaaac ataaacaacg ttgggcgggg
    16321 gcaatctgct gttttgtcct cttcattgtg gtgtgccttt ttctggcgac gcacatgaaa
    16381 ggcgcttttc gggctgccgg gcatcctgaa atcggcttgc tatttttcat tcttcctgga
    16441 gcagttgcca gtttcttttc acagcgtaga gaagtcctga aacctctatt tggcgcaatg
    16501 ctggcggcac cctgttcaat gctcattatg cggctgtttt tttcaccgac gcgctcattc
    16561 tggcaagagc tggcatggtt actaagcgcg gtgttctggt gtgcgctggg ggcactgtgt
    16621 ttcttattta tcagtagttt gtttaaacca cagcacagaa aaaatcagta aagccctcaa
    16681 cgcgagggct tgtcagacga tcaggcgtcc agattttctt tcacccatgc agcaaaatcg
    16741 gtatagccgc cgatatgttg ctgatcgaca aaaatctgcg gcacggtttc tacgggttta
    16801 cctgcctttt gttgtagatc ttctttagtg atcccttccg cacgaatatc tacatactga
    16861 tactgaaaat catcgcgttc attgctcaat ttctcagcca gatcttttgc acgcacacag
    16921 taagggcaac ccgaacgacc aaaaataacg gtttgcatta tttctctcct catagattta
    16981 tgcctgtaat gatcacgcta aaatgtattc gctgaaagta ggtttaacct gttgcattaa
    17041 ttgctaaaag ctataactgt taaacacaat acagtgaaaa gttttagact gaaggctcac
    17101 tttgcagagg gaagcgtatg cgcgcgatcg gtaaattgcc taaaggcgtg ttgatactgg
    17161 aatttatcgg aatgatgcta ctggcggtgg cgctgctgtc ggtaagcgac tccctgtcgc
    17221 tgcctgagcc attttctcgg ccagaagtgc agattctgat gatttttctc ggtgttttgc
    17281 tcatgcttcc cgctgcggtg gtggttattc ttcaggtggc aaaacgtctt gccccacagc
    17341 tgatgaaccg tccaccgcaa tattcacgtt cagaaagaga aaaagataat gacgccaacc
    17401 attgaactta tttgtggcca tcgctccatt cgccatttca ctgatgaacc catttccgaa
    17461 gcgcagcgtg aggcgattat taacagcgcc cgtgcgacgt ccagttccag ttttttgcag
    17521 tgcagtagca ttattcgcat taccgacaaa gcgttacgtg aagaactggt gacgctgacc
    17581 ggcgggcaaa aacacgtagc gcaagcggcg gagttctggg tgttctgtgc cgactttaac
    17641 cgccatttac agatctgtcc ggatgctcag ctcggcctgg cggaacaact gttgctcggt
    17701 gtcgttgata cggcaatgat ggcgcagaat gcattaatcg cagcggaatc gctgggattg
    17761 ggcggggtat atatcggcgg cctgcgcaat aatattgaag cggtgacgaa actgcttaaa
    17821 ttaccgcagc atgttctgcc gctgtttggg ctgtgccttg gctggcctgc ggataatccg
    17881 gatcttaagc cgcgtttacc ggcctccatt ttggtgcatg aaaacagcta tcaaccgctg
    17941 gataaaggcg cactggcgca gtatgacgag caactggcgg aatattacct cacccgtggc
    18001 agcaataatc gccgggatac ctggagcgat catatccgcc gaacaatcat taaagaaagc
    18061 cgcccattta ttctggatta tttgcacaaa cagggttggg cgacgcgcta aaaccgccac
    18121 gtcgatgtat gatacgcggg cttttgacca ggtctgacag agaggtgcag ggtgaaaatt
    18181 gccatattgt cccgggatgg aacgctctat tcgtgtaagc ggctgcgtga agccgctata
    18241 cagcgcggtc acctggttga aattcttgat ccgctttctt gctacatgaa cataaatcct
    18301 gcggcgtctt ctattcacta caaaggccgc aagttacccc attttgacgc agtgatcccg
    18361 cgtattggca ccgccattac cttttatggg acggcggcac tgcgccagtt cgagatgctg
    18421 gggagctatc cgctcaatga gtcggtcgcc attgcccggg cgcgtgacaa attgcgttcc
    18481 atgcaactgc tggcgcgtca gggcatcgac ctgcctgtca cgggcattgc gcattcgccg
    18541 gatgatacca gcgatttaat cgacatggtc ggtggtgcgc cgctggtggt caagttggtt
    18601 gaaggcacgc agggaattgg cgtcgtgctg gcggagacgc gtcaggcggc ggaaagcgtg
    18661 attgacgctt tccgcggtct gaacgcgcat attctggtgc aggaatatat caaagaggcg
    18721 caagggtgcg atatccgctg tctggttgtt ggcgatgaag tggtcgctgc gattgaacgg
    18781 cgggcgaaag agggcgattt tcgttccaat ttgcatcgtg gcggcgcggc aagcgtcgcc
    18841 agtatcacac cacaggagcg tgaaatcgcg ataaaagccg cgcgaacgat ggcgctggac
    18901 gttgctggtg tggatattct gcgtgctaat cgcgggccgt tggtgatgga ggtgaatgcg
    18961 tcgccggggc tggaaggaat agaaaaaacc accggtatcg acatcgcggg taaaatgatc
    19021 cgctggatcg aacgccacgc tacgacagaa tattgcctga aaacgggtgg ttagtcgcaa
    19081 tcacattact gatcatggtt ttgcctgcgc tttttgcgta agctgtgccg gtctttttat
    19141 cgaaagaggt tgtacaaaat tatgacatcg ctggtcgttc ctggtctgga tacgctgcgt
    19201 caatggctcg atgacctggg gatgagtttt tttgaatgtg ataactgtca ggctctgcat
    19261 ctgccccata tgcagaattt cgacggtgtc tttgatgcca aaatcgatct gatcgataac
    19321 acgatcctgt tttctgccat ggcggaagtc cgaccttcag ccgtattgcc gctggcggcg
    19381 gatttatctg ccatcaatgc cagttcgctg accgtgaaag catttcttga tatgcaggat
    19441 gataatctgc caaagctggt ggtttgccag tctttatccg ttatgcaggg cgtaacctat
    19501 gagcagtttg catggttcgt gcgtcagagc gaagagcaga tttcgatggt cattcttgaa
    19561 gctaatgccc atcaactgct gttaccgact gacgatgaag ggcaaaacaa cgttaccgaa
    19621 aactattttc tccactgata attcctttcg agcacgcagt cgctgccgca gtgactgcgc
    19681 gctgcaaaat tatctgctgt ttttaacctt ttcttaaaga ttatttcact tatcttgtgt
    19741 cgatttggct ttatcacata gagcaaatac gcataaaaat ttgttaaata ccgtttttta
    19801 atccgagcta tagtctcaaa ccctggctaa agttattctt gcgatgcttt tatatagcga
    19861 gcagtgctgg ccgggagaga gttctctttt cttacaccgc gccgataaaa aaatatgcac
    19921 gtttattgca tatctttcag tgtgacaact tttgttcgtt tgttaacgaa ctttcagaag
    19981 gaaagagata tgaccgcctt aaataaaaaa tggctatcgg gtctggttgc gggtgctctg
    20041 atggccgtct ctgtcggcac gctcgcggct gaacaaaaaa cactccacat ttataactgg
    20101 tctgattata tcgcccccga cacggtggct aactttgaaa aagaaaccgg tattaaagtc
    20161 gtctacgatg ttttcgactc taacgaagta ctggaaggca aattaatggc gggtagcacc
    20221 ggctttgacc tggtggtgcc gtcagcaagc tttcttgaac gccagttgac tgcgggagtt
    20281 ttccagccgc tggacaaaag caaattgccg gagtggaaga atctcgaccc ggagctgctg
    20341 aagctggtcg ccaaacacga tcccgacaat aaatttgcta tgccctatat gtgggcgacg
    20401 accgggattg gctataacgt cgataaagtc aaagcggtgc tgggcgaaaa cgcgcccgtc
    20461 gatagctggg acttgatcct caagcctgaa aatctggaaa aactgaaaag ctgtggcgtc
    20521 tctttcctgg atgcgcctga agaagttttt gctaccgtgc tgaattatct cggcaaagat
    20581 cccaacagca ctaaagcaga tgattacacc ggaccggcaa ccgatctgct gttaaagctg
    20641 cgcccgaaca ttcgttattt ccattcatcg caatacatta acgacctggc aaacggcgat
    20701 atttgcgtcg ctatcggctg ggcaggcgat gtctggcagg cgtcaaaccg cgcgaaggaa
    20761 gcgaagaatg gcgtgaatgt ctctttctcg attccgaaag aaggggcgat ggcgttcttt
    20821 gatgtcttcg ccatgcctgc ggatgccaaa aacaaagacg aagcctatca gttcctgaat
    20881 tacctgctgc gcccggatgt agtagcgcat atttccgacc atgtgttcta tgccaacgcc
    20941 aataaagcag ccacgccgct ggtgagtgcg gaagtccgtg agaacccggg tatttatccg
    21001 cctgcggatg ttcgtgcgaa gctgttcact ctgaaagtgc aggacccgaa aatcgaccgt
    21061 gtgcgcaccc gcgcgtggac caaagtgaag agcggaaaat aatccgcagc cttagatgcc
    21121 gggcgggcgc accacacccg ccggcaattc gcaccatcat ggtgcgcttg cacacattca
    21181 atgccggaga gctgccgtga atgacgctat ccctcgcccc caggcgaaaa cccgtaaggc
    21241 gctgacgccg ctattagaaa tccgcaacct gaccaaatcc tacgatggcc aacatgcggt
    21301 ggatgatgtc agcctgacca tctacaaagg tgaaatcttc gcgctactgg gcgcatccgg
    21361 ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttt gaacaacctt ctgccggaca
    21421 gataatgctt gatggcgtcg atttgtcaca ggttccgcct tacctgcgcc ccatcaatat
    21481 gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg
    21541 cctgaaacag gacaaactac cgaaagcgga aattgtcagc cgggtcaatg agatgctcgg
    21601 cctggtgcat atgcaggagt tcgccaaacg caaaccgcat cagctttccg gtggtcagcg
    21661 acagcgtgtg gccctggccc gaagccttgc caagcgcccg aaactattac tgctcgatga
    21721 gccgatgggc gcgctggata aaaagctgcg tgaccggatg cagcttgaag tggtggatat
    21781 tctggagcgc gtcggtgtga cttgtgtgat ggtcacccac gatcaggaag aggcgatgac
    21841 catggcgggg cgcatcgcca ttatgaatcg tgggaaattt gtccagattg gcgaaccaga
    21901 agagatctac gaacatccga ccacccgcta cagtgctgaa tttatcggtt cggttaatgt
    21961 ctttgaaggt gtactcaaag agcgtcatga agatggcctg gtgctttatt cgccggggct
    22021 ggtgcatcca ctgaaagtcg atgtagatgc ttcggtggtc gataatgtgc cggtgcatgt
    22081 ggcgctgcgc ccggaaaaaa tcatgctttg cgaggaacct cccgctgacg gttttaactt
    22141 tgcggtaggt gaggtgatgc acattgccta tctcggcgat ctttcggtgt atcacgttcg
    22201 tctcaagagc ggacagatga tcagcgctca gctacaaaac gctcatcgat atcgcaaagg
    22261 gttaccaacc tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct
    22321 gacggtttaa ggagcaaaga tgagtacact tgaacctgct gcccagtcga aaccgccggg
    22381 cggatttaag ctgtggttgt cgcagctgca aatgaagcat gggcgcaaac tggtcattgc
    22441 gttgccatat atctggttga tcttgctgtt tctgctgcca tttctgattg tctttaaaat
    22501 aagcctggcg gagatggcgc gcgctattcc accttatacc gaactgatgg agtgggctga
    22561 cgggcaactg tccatcactc ttaatctcgg taattttctg caactgaccg acgatccgct
    22621 ctatttcgat gcttatctcc agtcgctaca ggtggctgcg atttcgacat tttgctgttt
    22681 actgatcggc tatccgctgg cgtgggcggt ggcgcacagt aagccttcga cccgtaatat
    22741 tttattacta ctggtgatcc tgccgtcgtg gacctcgttt ctgatccgcg tttatgcctg
    22801 gatgggaata ttaaaaaaca acggtgtgct gaataatttt ctgctgtggc tgggggttat
    22861 agaccagccg ctgaccattc tgcataccaa tctggccgtt tatatcggca ttgtttacgc
    22921 ttacgtgccg tttatggtac tgccgattta taccgcgttg attcgtattg attattcgct
    22981 ggtggaagca gcgctggatc tcggtgcacg accgctgaaa acgttcttta ctgtgatcgt
    23041 gccgctgact aaaggtggga ttattgccgg atcgatgctg gtgtttatcc cggctgtggg
    23101 cgagtttgtg atcccggaac tgctcggtgg cccggacagc atcatgatcg ggcgcgtgct
    23161 atggcaagag tttttcaata accgcgactg gccggtggcc tcggcggtag cgatcatcat
    23221 gttgctgctg ctaattgtgc cgataatgtg gttccacaaa caccagcaaa aaagcgtggg
    23281 agaacacgga tgaataattt accggtagtt cgttcaccct ggcggattgt gattttgctg
    23341 ctgggcttta cctttctcta cgcgccaatg ctgatgctgg ttatctattc gtttaacagc
    23401 tcgaagctgg tgacggtgtg ggccggctgg tcaacgcgct ggtatggtga gttattgcgc
    23461 gatgacgcga tgatgagtgc ggttggttta agcctgacaa ttgctgcctg tgcggcaacg
    23521 gcggcggcga tcctcgggac tattgcggcg gtggtgctgg tgcgctttgg caggtttcgc
    23581 ggatcaaatg gctttgcctt tatgatcacc gcgccgctgg tgatgccaga tgtcatcacg
    23641 ggcttgtcgc tgttgttatt attcgtcgcg cttgctcatg ccattggctg gcctgcggac
    23701 cgcggtatgc tcaccatctg gctggcgcat gtcacttttt gtacggctta tgtggcggtc
    23761 gttatttctt caagattgcg ggaactggat cgctcgatag aagaagcagc gatggatctc
    23821 ggtgcgacgc cgctgaaagt gtttttcgtc attacgctac cgatgatcat gcccgcgatc
    23881 atttctggct ggttactggc ttttactttg tcgcttgatg atctggtgat cgccagcttt
    23941 gtttccgggc cgggagccac cacgttaccg atgctggtct tttccagcgt gcggatgggg
    24001 gtgaatccgg aaatcaacgc ccttgcaacg ttaattctcg gtgcggtcgg aattgtcgga
    24061 tttatcgcct ggtatctgat ggctcgcgca gaaaaacagc ggatacgcga tatccagcgt
    24121 gcaagacatg gctgaagaca ctaaaatttg ccaacctggc tacataatgc cgcgcatgcc
    24181 gcggcattgt tttcatggaa gacgaaacgt tgggattttt taagaaaaca tcttcatctc
    24241 atgctcgcct gaatgtgcct gcgctggtgc aggtggcggc gctcgccatt attatgatcc
    24301 gtggcctcga cgtgctgatg attttcaata cgctgggcgt gcgcggtatt ggcgagttca
    24361 ttcatcgcag cgtacaaacc tggagtttaa cgctggtctt tttaagcagt ctggtgctgg
    24421 ttttcattga gatctggtgt gcgttttcac tggtgaaagg gcgtcgctgg gcgcgctggc
    24481 tatatctgct gacacaaatc accgccgcaa gttacttgtg ggcggcttcg ctggggtacg
    24541 gttatccgga gctgttcagc attcccggtg aatcaaaacg tgaaatcttc catagcctga
    24601 tgctgcagaa gctgccggat atgctcatcc tgatgctgct gttcgttccc tcgaccagtc
    24661 ggcggttctt ccagttgcaa taatgtgtat aatcgtcgcc cctgatgatg tgaaggtcaa
    24721 tgtatgcagt gcgcacttta cgacgcgggt cgctgtcgtt cctgtcagtg gataacgcag
    24781 ccgattccag agcaactctc cgctaaaacc gccgatctta aaaatctgct cgccgatttt
    24841 ccggttgagg aatggtgcgc gccggtgtca ggcccggaac aagggtttcg taataaagcc
    24901 aaaatggtgg tgagtggtag cgttgaaaaa ccactgctcg gtatgctgca tcgcgacggc
    24961 acaccagaag acctttgtga ctgcccgctt tatcctgcct catttgcgcc cgtttttgcg
    25021 gcgctaaaac cgtttatcgc ccgtgcgggg ctgacaccct acaacgtggc gcgtaagcgt
    25081 ggcgaactga aatacattct gctgactgaa agccagagcg atggcggcat gatgctgcgc
    25141 tttgtactgc gttctgaaac caaactggca caactgcgta aggcgctgcc gtggttacag
    25201 gaacaactac cgcaactgaa agttattacc gtcaatattc agccggtaca tatggcgatt
    25261 atggaagggg agacggagat ctaccttact gaacaacagg cgctggcaga gcgttttaat
    25321 gacgtaccgc tgtggatccg tccgcaaagt ttcttccaga ctaatccggc ggtcgccagc
    25381 cagctgtacg ctaccgcgcg cgactgggta cggcagctgc cagttaaaca tatgtgggat
    25441 ctgttctgcg gtgtgggtgg ctttggttta cactgcgcga cgcctgacat gcagttaacc
    25501 gggatcgaaa ttgcatcaga ggccattgcc tgtgcaaagc agtcagccgc tgaactgggc
    25561 ttaacgcgtt tgcaatttca ggcgctggac tccactcagt ttgccaccgc tcagggggag
    25621 gtgccggagc tggtgctggt taatccgccg cgccgcggca ttggtaaacc gctatgtgat
    25681 tatctctcaa cgatggcacc gcgttttatt atctactcca gctgtaacgc ccaaaccatg
    25741 gcgaaagata tccgcgaact gccaggttac cgcatcgaac gggtacagct ttttgatatg
    25801 ttcccgcaca ccgcgcacta tgaagtgatg acgctgctgg tgaagcaata aaaaagccgc
    25861 aggtgcggct tcagattgct gatcaggtgc gctttgttca tgccggatgc ggcgtgtacg
    25921 ccttatccgg cctacaaaat catgctaaat caatacattg cagagttgat gtgggccaga
    25981 taagcgcagc gcatcaggca atcttacttt tgtcatcagt ctcaattact gcgggaacca
    26041 ctggtcattg attttctgat atgtaccgtc agctttaatt gctgccagcg cgttattcag
    26101 tttttccagc agagctttgt tatccggacg tacagcgata cccaggccgg tgccgaagta
    26161 ttgcggatcg gtcacttttt cagtcgcaac gcccagttgc ggattggttt tcagccattc
    26221 gtttaccacc gctgtgtcac caaatacccc atcaatacga ccatttttca gatcgataaa
    26281 ggcattctga taactgtcat aggagacagt tttcacttcg gggtgctgat cctgaatata
    26341 tttctggtgc gtggtgccgt tttccatccc aatacgtttg cctttcagat cggcaaacgt
    26401 tttgtaggta tcttttttgg caatcacgac ggctgagttt tcatagtagg gcgtggtaaa
    26461 cgatacctgc ttgctacgct cgggcgtgat atccataccg gagattacgg cgtcatattt
    26521 tctgaatttc agtgacggga tcaggctgtc gaacgcgtga ttagtaaaag tacattctgc
    26581 ctgcatttgt ttgcacaagg cttttgccag atcgatatca aagccgacaa tctcattatt
    26641 agcacctata gattcaaagg gtggataggt ggctgaaacg ccaaaattga ttttctctgc
    26701 ggcagaagca ccgaaagtaa aggaagcaag taaagcggca agaactaact ttttcatgat
    26761 ggaactcccg tctgtcaatc ttatgatttt tggccgtgtc tgcggcatgg gataacaatg
    26821 ccatcaagtg aatttatatg caataaatat gattaaataa tttaaatgaa ataaaaaaag
    26881 acggataact tagtgagttg tccgtcttca ttataagaat ttatgcacta tgtaggccgg
    26941 ataaggcgtc cacgccgcat ccggcacagg caccgtgctg atgtctgatg cgacgctggc
    27001 gcgtcttatc agacctacaa acccccccgg cgaatgtacg cagctacatt aattccgccg
    27061 ttcgaatgcc agtgctttgc gctcgatcag acgcatcatc agcgtcagca ggccgttgac
    27121 gaccaggtaa ataatccctg ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa
    27181 caactggctg tatcccatca cttccatcag cgtaatggtg taagccagag aggtactttt
    27241 gaacaccagc accacttcgt tggaataaga agagagcgag cgtttaaagg catacggcag
    27301 caggatcgcc agcgtatctt ttttgctcat tcccagggcg ctacaggact gccactgacc
    27361 ttctgggatc gcacgaattg caccgtaaaa cagctgcgtg gtatacgccg cactgttcag
    27421 cgataacgca atcagcgcac ataaccacgg ttctgacaac aaatgccaca gtgccggata
    27481 ctcctgcaaa gtcggaaact ggcccggccc gtaataaatc aggaagatct gcaccagcag
    27541 cggcgtaccg gtaaacagcg tgatataacc ccgcaccagc cacaccagca ccggcgtttt
    27601 cagcgtcagg atgatggtaa aaatcaatgc cagaatcagt gccacaatca gcgaggcaac
    27661 ggttagcgtc aggctggtgt gtagcccttt catcagttcg ggtaaatact caaacattag
    27721 ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg
    27781 agcagggtga tcaccaggta aatcgccgcc gccacaatgt accaggtaaa tggttcctgg
    27841 gtacgagtag cgatgctttt tgtttgcagc attaaatcat tcacactaat caaactgacc
    27901 agcgcggtat ctttcagcag caccagccac tggttaccga ggccaggcag cgcatgacgc
    27961 cacatctgcg gcatcaccag acggaaaaag atagccgatt tcgacagccc cagcgcctga
    28021 ccagactccc actgacccac cggcaccgct ttcaacgcgc cccgaagcgt ttgcgaggca
    28081 taggcggcat acagcagtga cagagcgatg acaccacaaa ggaacgggct aacgtcgaag
    28141 ttctcaatgt ccatctgcac tgggatctgc acgaacccaa gattgatagt gaagccatcc
    28201 gaaagcgtca gcagcagctg cgaggagcca aaatagataa acagcaccac cagaatttct
    28261 ggcaggccac gcagaatggt taccagcgct gaacctgccc acgcgacagg acgccatttt
    28321 gccgactccc ataccgcaaa gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg
    28381 gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa aaaattcatt catcaggaat
    28441 tacttctgga accatttgtt gtagatggtt tcgtaagtgc catctttctt cactttttcc
    28501 agcgcagtgt tgagtttctg ctgcagctca gtgttgccct gacgtaccgc gatgccgagg
    28561 ccagtgccga agtaatcttt atcggtcact ttgtcgccca ccgccgccag tttcgggtta
    28621 tctttcagcc actcagtgac cactgcggtg tcaccgaaga cgccgtcgat acgcccgttt
    28681 tgcagatcca gttttgcgtt ctggtagctg tcatacggaa cggtagtgat ttccgggtgc
    28741 ttatccataa tgaatttctg gtgtgtcgtc ccgttctgaa cgccgacttt tttgcctttc
    28801 agctgatcaa cactggtgta tttgccttgc tgacccacaa acagggcaga gttgtcatag
    28861 tacggggtgg taaacagtac ctgcttttca cgctccggag tgatatccat gcccgccatc
    28921 actgcttcta cgcgacggaa tttcaggctt gggatcaggc tgtcaaacgc ctggttagag
    28981 aaagtacagg ttgcatcaat ctctttacac agtgcctgtg ccaggtcgac gtcaaaacca
    29041 acgatctggt tgtttgcatc aatcgattca aacggaggat aggaggcttc ggtagcaaaa
    29101 cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga
    29161 acttttttca ttgttgttat cccgaatctt agtgagagag atagttttta aatgcttcgg
    29221 tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac gatatgacca ttttccatat
    29281 acaccactcg gctggcggtt ttacgcgcca cttcaacttc gtgggtgacg atcacctggg
    29341 taatattcgt ttctgccagc tcacgaatga tgctgacgat ttgtgccgta atttccgggt
    29401 ccagtgcggc ggtcggttca tcgaacagca gtacctgcgg ttccatcatc aacgcacggg
    29461 caatagcaac acgctgctgc tgaccaccag aaagatgcag cgggtaacga tcgctataag
    29521 gtttgagacg cagacgttcc agcagttttt ctgcacgggc cagcgcctga tctttactca
    29581 accccagtac acggcagggc gcttcaatca ggttttgctg cacggtcaga tgcggccaca
    29641 ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag
    29701 agggtgtttt ggtgaaatcg aaatggttgc ctgcaatgtt gagcgtaccg gagcgcggca
    29761 tctcaagcag attgagtaca cgcagcagcg agcttttacc cgcgccgctg gggccaagta
    29821 acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc
    29881 cgtagaagca attaatgccg tttaattgaa tactcattga cactcgtata ctggcagtct
    29941 gatagctatt gaggtcgaag atagtacctt tgacagaata attatgcaat atttctgctt
    30001 taaaagttaa aagcaaagcg cattattcaa taaacatagc acaaaataac gggggcggtg
    30061 gtcggcgagc ataaatgtcg gcattcctca cgaaatgccg gacaatttac ggggtttatt
    30121 ggttgatcaa ggcgttagcg attctcgatg gactgacgga gcgtacccgc cgtggcatga
    30181 acgctgccgc ctaaatagcg tacatcgtcg atcacccaac actggccttc ctgaatcatt
    30241 aacacttcat cctgccagct ctgatcaccc tgttttagat ccacacgcag aggaatattt
    30301 cgcgcatcac gattcgggat tgttgaggca ctggcaacac gggcgctctc tggcaaggtt
    30361 gtgcgactgg agaatggatc gttggtcagt agttcccgat ggttattatc ccgggaggca
    30421 tcgctaagca gtgtcgccag tttgtcgctc agataagggc gcaaggcggt gatgtcgttg
    30481 ctgcggtgca aaatgcggta gtcataaaat tgctgggcca cgttatccgg gcctccttca
    30541 acgcaaggac cactgcgtgt gccgttatct ttataagctg gagtgactgt ggtgcaggca
    30601 ctgaggagca gtgcgcaggg gataagcatt gtcaatttgc tgtagcgcat aatgatttcc
    30661 ttataagcga tcgctctgaa agcgttctac gataataatg atatcctttc aataatagcg
    30721 tatcagtctg ataatgcttt tgagatcgaa ggcttagcaa acaaggagat cgatcatgca
    30781 attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg gtgtggtgac
    30841 cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgctggta tccgcgatat
    30901 cgttggcgga cgctccggtg cctacgaaaa agaattacgt aaagcgcggg agatcgcctt
    30961 tgaggagctc ggctcccagg cgcgggcgct gggggccgat gccgtagtcg gtattgatat
    31021 cgactacgaa acggttgggc aaaacggcag tatgctgatg gttagcgtca gcggtacggc
    31081 ggtgaaaacg cgtcgatgag aagagtcttc tggctgattg ccgccgctct gttactggca
    31141 gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga tacccgacgc
    31201 caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac cgcagatgat
    31261 tttgatagct cgctggcgac actgaccgat aagcaggtca gctcgcatta tctggtccct
    31321 gcggtaccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc ggaacaagaa
    31381 ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa cgacacctct
    31441 attggcattg agctggaaaa ccgtggctgg caaaaatcgg ccggagtgaa atattttgcc
    31501 ccgtttgaac cggcacagat tcaggcgctt attccactgg cgaaagatat tattgcccgt
    31561 tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca gcgcaaagac
    31621 gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg tgcctggccg
    31681 gatgcgcagc gggttaactt ttaccttgcc gggcgcgcgc cgcacacacc ggtagatacc
    31741 gcgtcattgc tggagctttt ggcgcgctac ggttatgacg ttaaacctga tatgacaccg
    31801 cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac gttatataac
    31861 ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggaaaa atacgggcag
    31921 gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc gataccttca
    31981 tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat atccagcgta
    32041 aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt tgctgacttg
    32101 cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaggg gacggaacga
    32161 atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg cagtgtgcga
    32221 tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca ggcttcctgg
    32281 cttgccagcc acatggcgtg cacggcattt tcatagtagg tcatatcgac cagcgcactg
    32341 ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag acggggaata
    32401 aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa gcgcgtttgt
    32461 ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct gcgggcaaac
    32521 tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg gtgataatcg
    32581 aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc gacagcccat
    32641 tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg tgtcccccag
    32701 ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat cactttagct
    32761 tgtgatgaaa ccagctcggt cagatccgcc ggaacaaact ctgcgcccat tttttccagc
    32821 aatttgccca ttgcctcgtt gcgaccggtc gctcgcacgc tgatgccttt ctggcataaa
    32881 aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac cttcatatca
    32941 atccactgtt gttgagaaaa taacgtgcgc attcttccgt gatttccccc atgatgcaat
    33001 gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt tctgccagtc
    33061 tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc atcaataacc
    33121 agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg atagtacgat
    33181 agtcgccttt atcttccagg ctaaaacaca gtcgtcccag ccccggcgct ttcatgccaa
    33241 ataacaacgt aagttgtttt tccggttcaa cgacaatcac tttccagcta tctaccgcat
    33301 cgccagtctg taaatattcg cgctccgggc ggcctttcgc cagcttatga ccgatcgcgc
    33361 ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc tctttaccgc
    33421 cgatttggtt cactacctgc cataaagcag caaggctggc ggacgtttta acggtaaacc
    33481 ccgcctgttt ggcaaaataa ccgtactccg gtcgccagcg ggcaaaggcc tgagcgtcgt
    33541 agccccagtc gctggagttg accagttttt cctcctcttt caacgtgcta cgtaccgcgt
    33601 catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca tccgccagca
    33661 gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg gaagtaatca
    33721 cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc aaccagcggc
    33781 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
    33841 cggcggcttc gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc accagatagt
    33901 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
    33961 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa
    34021 cgataattcc ggcccgaagt tcggtcacag gtacattcgc ttcacgaaga atgtccgccg
    34081 tagcctgacg agcacgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga
    34141 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct
    34201 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg
    34261 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
    34321 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga
    34381 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
    34441 cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag
    34501 tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca
    34561 atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca
    34621 ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
    34681 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
    34741 acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg
    34801 ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt
    34861 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
    34921 tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
    34981 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca
    35041 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
    35101 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
    35161 gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac
    35221 agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc
    35281 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
    35341 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
    35401 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca
    35461 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
    35521 agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc
    35581 aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaac
    35641 tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc
    35701 agggcttcat caacttcaga cgctttttct acacggatac ccgtaatgcc gcacgcttcg
    35761 gcaatgcggg caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata gccaccagct
    35821 ttcatctcca tcgccacaaa gcccagcacg ctgttgttaa agacgacaat tttcactggc
    35881 agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
    35941 cacatggcga ccacctgacg ttctggctct gtcgcctgcg cacccagcgc ctgcggcatg
    36001 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttc
    36061 agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat ggcgtcatcg
    36121 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
    36181 ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc tttatccaga
    36241 aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt tgatttgata
    36301 tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg attgatatca
    36361 atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg agtaccgagt
    36421 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaagcc gattaacccg
    36481 gtcattccaa catcatacgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga
    36541 acaataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
    36601 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
    36661 aactcttctt cttccggcgt cacgattggt tgtggcgcat gataccagtg cgtggttgcc
    36721 ccttctggcg caggttttaa cgccacgtcg cctggtaaca cgacaaccga aacgccacgg
    36781 ttaagcaccg ctttgcgcat ggcaatcgcc agtacttgtg gaatctgctc cgggctggaa
    36841 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
    36901 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga
    36961 ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccggggcc gcacgatccg
    37021 gcacagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa ggccgccact
    37081 tcttcgtggc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcactaaga
    37141 ccgttcagag agtcgcctgt gactccccag atgcgtttca cccctgccga ttcgagtgtt
    37201 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
    37261 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
    37321 ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa
    37381 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
    37441 agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg
    37501 caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt
    37561 agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
    37621 ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
    37681 ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
    37741 acggtacgtg aagctaagtc aggtacacct gccagcagtt cgcgagtgag acgaccagcg
    37801 ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca
    37861 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggccagt
    37921 ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc
    37981 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
    38041 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg
    38101 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
    38161 gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg
    38221 cgatatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
    38281 gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
    38341 ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
    38401 caaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
    38461 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgacg atcgctttct
    38521 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt gacaccgcag cccagtttct
    38581 ctgccagagt gacagccaga ataatcgctg agtaagcatc gttacattga cctgcatcta
    38641 ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
    38701 aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt
    38761 ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
    38821 ggctcaccag atcaatcagc gtatccgctg cgccaagcag cgtctggcgg ccaaagccca
    38881 cggtgatcag gtgcggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga
    38941 tgaccgcaga aaaatcttcg ccatccagat gacgcacacc cggccagcca acgatgctgc
    39001 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
    39061 tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt
    39121 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
    39181 cgccgtgggt gtaaacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
    39241 ctttgagatc gtgaccggaa atcaggatgc atttacccgc cgtcgctttg acgttgacct
    39301 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
    39361 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
    39421 aggtccccag ccacgccatg attttatgat actgggcata aatatcgttg tcgtattgac
    39481 cgagaacatg cgcgtgttcc atataggccg ctgcaccttt caggccatac aggcacagca
    39541 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
    39601 ctgcctgacg ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt
    39661 tatcgacgcg cgcgttggca tctacagcca ggcattgcgc tttcagcgcc tcgcgcaggg
    39721 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
    39781 ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac
    39841 gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
    39901 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg
    39961 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
    40021 ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca
    40081 actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta
    40141 ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
    40201 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
    40261 cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg
    40321 gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat
    40381 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
    40441 ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc
    40501 aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
    40561 ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat
    40621 taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat
    40681 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
    40741 cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat
    40801 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
    40861 cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
    40921 aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg
    40981 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
    41041 gaaaacccag tccttgcaaa gatgaagtcg aaatgcgcga tgacacacta ctgaaagcgg
    41101 aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca taaggagcag tagagtattc
    41161 gttttcattt aaagatattc ttgcgcttta attacaaact gcaccgatgt tggtggcgtc
    41221 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    41281 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agtcgagggc
    41341 accgcgcagc ggcgattttg ttcgccagag cccgggggtg cagggggcgg cggcgattgg
    41401 ccgccccctg cgcgctcctt gcgccagtgg caatatgtgg cttagctcat gaaaggagcg
    41461 caacaagatg atgaatcaac atataacaac atcttaaaaa aaggcctgac attacgccag
    41521 gccttctgcg ttaattaatc acgcttttcc agcagggtcc ggtaaatcag accaccgata
    41581 atgccgccga caattggcac cacccagaag aaccacagtt gttctaatgc ccagccgccc
    41641 tggaagatag caaccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata
    41701 ctaattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa acctgccggc
    41761 gcgaatttgt cggttgtgcc gtggatcacc aacaggaaac ctgcactcaa taccagttca
    41821 actaccagcg cggaaagcat ggaataaccg cctggtgaat gctcgccata accgttagaa
    41881 gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa atacagcagc
    41941 gccgctgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc
    42001 ggaaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa
    42061 atatgaccaa cagcaaaggc catcgtcaga acggtcagac cgaacgccaa cgccacgccg
    42121 gcaaaaccaa tgcctaattc cgggaagcct gcggccagta cagcactacc acagccacca
    42181 aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca
    42241 attaaaaatt gaccctgtga aaaatatggt cgttttatag ggtcgtcgta aaaaagtgac
    42301 gacggaaata atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa
    42361 tccagtaaat ttgatttagg gcaacagcgg gttgccccat atagtcattt gtctgattga
    42421 cagtgtagtg caagcaaaag atttaatcct ttaggcgtaa taaaaaataa tttatcatgc
    42481 taattatttg attttgttgt ttttgcagac ttatcagcaa gagggagtat aacgcgatta
    42541 ttcgctcatt tttcagacat ttgccatgct taaatgtgat gtcatcacgt attagcaagg
    42601 cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag gggatatgat
    42661 tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt tgatgctcga
    42721 acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc tggacgcctt
    42781 aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg atttctggtt
    42841 cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga ccttccgcga
    42901 atcgctgcca ggccgacatc gggttcgccg ttatcggccg ctggaagcgt gctggacgcc
    42961 atgcaccgat ggctatcacc gtatttttta tcgtctggaa ggggagagtg cggaagacgg
    43021 cagcgtgatg acactgcgca gttttctcga taaagacgga catccgattg atgtcgagga
    43081 tattaacgat caggcacgcc atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc
    43141 ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gttcccaatg tggaagtcac
    43201 cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca catccgcaaa atctctctga
    43261 tggacagatt cgtcagggac tttccgcaat ggtgcagttg cttgagcatt atttctctga
    43321 gcagggggcc ggacaggcgc gatatcgttt aatgcggcgg cgggccagca atgagcaacg
    43381 aagctggcgc tatctggaca tcatcaaccg gatgattgac cgacctggag ggcgctcgta
    43441 tcgggttatt ttgctcggcc tgtttgctac tttattgcag gcaaaaggca cattgagact
    43501 ggataaagac gcgcgtccat tgttgctgat cgaagatcca gaaacccgtt tacaccccat
    43561 tatgctttca gttgcctggc atctgttgaa tcttctgcca ttgcagcgca ttgccaccac
    43621 caactcgggt gagttgcttt cgttaacgcc ggtagagcat gtttgccgac tggtacgtga
    43681 gtcctcgcgc gttgccgcct ggcgtctggg gccgagtggc ttgagtaccg aagatagccg
    43741 acgcatatcc tttcacattc gttttaaccg tccgtcatcg ctgtttgctc gctgctggtt
    43801 gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa ctggcgcgtc agtgcggaca
    43861 tcattttgat gccgaaggga tcaaggtcat tgagtttgcc cagtccgggc taaagccact
    43921 ggttaaattt gcccgccgaa tggggattga atggcatgta ctggtcgatg gcgatgaagc
    43981 agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg
    44041 agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt
    44101 ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctgcgcaa
    44161 aattatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat
    44221 ggaggccgga cgtcgtggtg tggactccgt accgacgctg ctgaaaaaaa tgttctcacg
    44281 cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg
    44341 ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat
    44401 ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca
    44461 tacgccgcca accgattccc agaaagcgtt gtaatcggca tggatcttgc cttctttatc
    44521 acgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac
    44581 ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg gaaatagccc
    44641 gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg
    44701 cagtccgcca ataaacatcg ttcttttccc ctgatattca cacagggtaa acgtgatctc
    44761 tgccagagga ataccttcgc tgttgcgaaa caggattgtg ctgtcacctt ctttatccat
    44821 tgagatcatc atggtcagct caagcgtgaa ctgctcgccg tttttgcctt ccagcttcgc
    44881 cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca tacatccgcg
    44941 gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc
    45001 aaggtaaggg cgatgcagac gcacaggcag tcgcggttgg cgcgttaaca acacattgag
    45061 attaggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca taatcaacga
    45121 gcgcagcaaa aatttctggc gaaaactacg gcgatgccag aatttacccg gccgacactg
    45181 tccacgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt
    45241 ccgttcagtt agctgcgaca tattcatgaa atcaatggtt atacatgacg tcgatttcac
    45301 cattgcgtat cttaaccaaa catcaatagt gtgattacta acgtaaattt tagggttttg
    45361 ttgatatttc gttgaagtta atgacccgga ttggcatatg gagtattcag aatatttatg
    45421 aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc tggtgatagt catcgccgga
    45481 ctgattacgt tatggagaat tcttaacgca cccgtgccga cttatcagac actgattgtg
    45541 cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag
    45601 gttgacgtgg gcgcgcaggt cagcggtcag ttgaaaactc tgtcggtggc gattggcgat
    45661 aaagtaaaaa aagaccagct tttaggggtt attgatcctg aacaggctga aaaccagatt
    45721 aaggaggtcg aagcaacgct gatggagcta cgtgcgcagc ggcagcaggc ggaagcggag
    45781 ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc tggcacaaac gcaggcggtt
    45841 tcacagcagg atctcgacac cgccgcgacg gagatggctg tgaaacaggc gcaaattggc
    45901 accattgacg cgcaaatcaa gcgcaatcag gcctctctcg atacggctaa aaccaatctc
    45961 gattacactc gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac cactctgcaa
    46021 ggccagacgg tgattgccgc acaacaagca ccgaacattc tgacgctggc agatatgagc
    46081 accatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa
    46141 aaagcctggt ttacggtact tggcgatcca ctgacgcgct acgaggggca aatcaaggat
    46201 gtactaccga cgccggaaaa ggttaacgac gctattttct attacgcccg ttttgaagtc
    46261 cccaacccca atggtttgct gcggctggat atgactgcgc aagtgcatat tcagctcacc
    46321 gatgtgaaaa atgtgctgac gatccctctg tcggcgttag gcgatccggt tggcgataat
    46381 cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc
    46441 gcacgtaacg ataccgatgt tgagattgtc aaagggcttg aagcgggcga tgaagtggtg
    46501 attggtgagg ccaaaccagg agctgcacaa tgacgccttt gctcgaatta aaggatattc
    46561 gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc accctcgata
    46621 tttatgccgg tgagatggta gcgattgttg gcgcttcagg ttccggtaaa tcgaccctga
    46681 tgaatattct cggctgtctg gataaggcca ccagcggcac ctatcgcgtc gccggtcagg
    46741 atgttgccac actggacgcc gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta
    46801 ttttccagcg ttaccattta ctttcgcatt taaccgccga gcagaacgtt gaagtacccg
    46861 ccgtctatgc tggtcttgag cggaaacagc gactgcttcg tgcccaggag ttgctgcaac
    46921 ggctggggct ggaagaccgt acagagtatt atccggcaca gctttcgggt ggtcagcaac
    46981 agcgcgtcag catcgcgcgg gcattgatga acggtggtca ggtaattctt gccgatgaac
    47041 caaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc
    47101 gcgatcgtgg gcatacggtg attatcgtca cccacgatcc gcaggtcgct gctcaggccg
    47161 agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag
    47221 tgaatgttgc tggcgggacg gagcctgttg tcaacacggt gtctggctgg cggcagtttg
    47281 tcagcggttt taacgaggcg ctgacgatgg catggcgggc gctggcagcg aataaaatgc
    47341 gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg
    47401 tggtgggtga cgccgccaaa caaatggtgc tggcggatat tcgttctatt ggtacgaata
    47461 ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc
    47521 tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg
    47581 ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgttgctgcc agtgccaatg
    47641 gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaagga aacaccttta
    47701 atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc
    47761 agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg
    47821 ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc
    47881 gtgtctggct accttacagc acgatgtccg ggcgagttat gggccagtcg tggctgaact
    47941 ccattactgt cagggtaaaa gaaggatttg acagcgccga ggcggaacag caactcacgc
    48001 gtttactttc actgcgccac ggaaagaagg atttctttac ctggaacatg gacggcgtct
    48061 tgaaaactgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg gtggcggtga
    48121 tttcgctggt ggtgggcggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc
    48181 ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac
    48241 agttcctgat cgaagccgta ctggtttgcc tggtcggtgg cgcgttggga ataacactgt
    48301 cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt ggtttttcac
    48361 cgttggcact gctgctggcg tttctctgct cgacggtcac cgggatttta tttggatggt
    48421 tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacga gagtaatttt
    48481 tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga
    48541 cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg
    48601 ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa
    48661 ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag
    48721 atgaaaccaa accctttggc attgttgaac cacttaacag tacccttttc catgcttcga
    48781 catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt
    48841 tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcgtt
    48901 tgacaggggc cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag
    48961 gaatgtgaca gatgtcgctg atgccaacga tagatgatag ttatttatca tctagtgttc
    49021 gcgggtgttc gtggtagtgt tttgtgtgca ttcatcgcta tgattataaa ggaaaatttt
    49081 attttccttg ttcgctcttg ttcgtcacgg tttgcccttg tactatgcac ggtgtagtca
    49141 atggtgtagt taattttgat gaccggaaac gcgaaacatg agcagcgcaa ttcatagact
    49201 tagcgacact cttttacgaa aattaagcgg atcaccaacc acaaaaaacg ccttttttaa
    49261 tgacggtggc aatcttagcg taagacattc cactagcggc ctgttaacct ggtatttcac
    49321 ttacagggcc ggaactggca ggcaggtatc accggaacgc ctgagactgg gcaactatcc
    49381 cgatctgagc ctgaaagcag ccagggaaaa agcggcacag tgtcgcgcct ggctggcgga
    49441 ggggaaaaat ccacgccatg agcttaatca cacagtgcag gaagcgttag cccccgtgac
    49501 ggttaaggac gcgctcactt actggcttga gtcgtatgcg aaggaaaagc gcactgacca
    49561 cgaatcactg aaaagccgta tcaataagca cataatcagc cagattggtg ctatgccgct
    49621 ggataaatgc gagctacgcc actggctggc ctgttttgac cagcttgcta agcgtaatcc
    49681 ggtatcagcc ggctttttgt tgcaggtatg caaacaggcg cttaagtact gccgtaaacg
    49741 tcgctacgcc gtcagtaacg ttcttgatga tatggtcgta ggtgatgtgg gaaaaaaagc
    49801 agaaataagc gagcgagtgc taacaaacaa ggagcttggc gagctactcc gcgcactgga
    49861 tgaaaaaatt ttcccgccgt actacagcgc tttaattcgc cttctgattg tcttcggatg
    49921 ccgtaccact gagctaagac gctccgaggt tcgggaatgg gattttaaag aaatgctctg
    49981 gactgtgccg aaagaacaca gcaaaacgaa ggtagcaata ttcaggccaa taccggaagc
    50041 gatcttgccg ttcgtcatga agctggtgga gcagaacagg cacacgggct tattgcttgg
    50101 agaactgaaa gggcaatcat ccgtagcaga gtacggaaga acggcacaca gacgcattaa
    50161 tcaagccccc tggtcgttgc acgacatccg gcacacattt acaaccatgc tgaacgattt
    50221 aggcgtggat ccgcatattg tcgaacagct tactgcccac cagatgccag gaatgcaacg
    50281 cgtctataac cactcccgtt atctcgatgc aaagcgtgac gctcttaatc tatgggtaga
    50341 ccgtctcgag cttctccaga acaatgatga aaaaatcgtt gttatgaccc cgcgaattta
    50401 cacccaaaat tattgacaaa ttacggctgt tttttcttta agagattaca gccgtgaata
    50461 aatcgtagaa tatcggaaaa tatctgcaaa tatcttaatt ggaatgtcgt cttctgggtt
    50521 tttattctct gtttcttgat tgttccaaca cttgatggtt tatctatttg tttttaatga
    50581 gataattcaa gtgtcatgca aaatgaagac atttttttgt tgcgtcgctt aaaaactcgt
    50641 gacacgattt taaacactaa cgagcttcta cgaaccttag cgaacaaagc ctttagcatg
    50701 gttgtgtttt taatttaaga ggtgaaaaag aatctaagta atatccgatc tcatatatct
    50761 acagctaaga gaaaagttta tatacgtaat ttttatgtag gtttttgtat gcgtgaaata
    50821 aatgaagatc gcgtaatccg tgaggatgaa tgtcgtaagt taactggagt gtgtcgcact
    50881 acacgctacg aactggagaa aaaagggagc tttccgtctc gccgtaatct tggtgggcgt
    50941 tctgtaggtt ggctgctgtc cgaggttatg gaatgggtta aaagccggga tcgcattaat
    51001 tcaggtaagg cagcgtaaag gggagcatat gacatatata acaaaggcga ccgggggcgg
    51061 tcgccaatgg atacacacta aacttgaacg catcaccaac aatgccacat ttgcggctgg
    51121 tgggcaatgt gatcagtcag atttggttcg tcccaaggtt tgcaacgaga gctttttcct
    51181 gtgctcttta aggaatttct caagagcaaa cgaacaaggt gcgaatcttt ctgattcatg
    51241 ctctatcttt ctgcgccgtc ttttccgtgc cggtgataat gttttggtca attctttatc
    51301 ggtcattgtg ttgtcctgca tagcaatgcg ccgtagttac tcacaccacg gcgctgatag
    51361 tgattattct gattctttgg ccttccggcg ctgttcatat tttaatttgc gtccacaggc
    51421 atctaagacc catgcggaaa aattagcact aggattagtt aatttttctt gttcaacgct
    51481 ggcatcaatc ccatctataa gctcatgtgg gaatcgaata tttttctttg ctgatttgtt
    51541 gtttgtgtta ccgctagaca ttgatgcacc tcacttaaat ggactttatg ggcgcacaca
    51601 atacagcaaa aaaaatgaga tgttaagtat tgacatgtgc gcacacatga ttttaatctg
    51661 tgcgcacagt ttgagttttg tcactcaaat taagcaacgc cccgcagtgc tcgcaacaca
    51721 tgcagggcgt ctaaccacca acgatagcaa gagtatcgag gtagctatga gaaatcatac
    51781 cacacacccg caagggcggg actcgcacaa cctgaataaa tacatctggc gttttatcgc
    51841 cctgagtacg gcacaaccgc gcgtgattac cattgaggcc agcagcgaac aggaagcacg
    51901 ccagcaatcc ccggctggct gcgtgatggt attcgccgcc cgtattcgtc agggggtatg
    51961 ccatgcctga tatgtcaaat taccagtacc tgattaatcc gcattttaac tgtgagcatg
    52021 atattgctaa aaaggtttat tccgctgcgg atggggctac tgacaatata tcaatggctg
    52081 ttgcgtcaat tggtagcctg atgtggcatg cgtcagaaaa tgaggactat gacgaaaagg
    52141 ctatgcgcat tgatatgggt aatatcggtt tgttactggc aatgcttggg cagtttgata
    52201 tttcgttacg gtgcaccatt gaaaatgcca cagatgcgtt aaatgccata aagaaagcga
    52261 atactgattc aaatcgggga taagtaatca tgagaacgta tttatctggc ttgactgcca
    52321 gcggttatgc acaccccaaa attatccccg gcgctattta tctggataag aacggtaaca
    52381 gagtaacggt aaaagaactg atgtttgacc gtgtgtattt tatccgtgat ggctattcat
    52441 ttcttagttc gctgaacgtg gagatcttta tttgtagatt ccggcgggaa atcccgactt
    52501 ccagaaataa ccatgtgtca cgtgtggatg tggataaaaa actacaggaa ctgaaaaaca
    52561 tgattgccgc gtggagagag cagaaatgaa aaaagcgcca aatttaaaac accagccgcg
    52621 tgacaaaatg acggaagtca tcatttttgc gggtagtgat gcgtgggcac atgcgaaaca
    52681 gtggcaggaa caggacgggc gactggctgg cgataacgtg ccacctgtct ggcttggaga
    52741 gcaacaactt gtcgaactgg acaacctgca aatcgtaccg gacggacgct atcgcgtgcg
    52801 tctctatcag gcggggttat tgcgtccggg gcttgttaat accatcgggc agaaactggc
    52861 agcggcaggt gtcagggatg ctgattatta ccctgaagga atgcacagcc agaaacggga
    52921 gaactggcgc gaatatctgg agcgtgagcg gggagagctg gcggaaaaga aaaaggtagt
    52981 tgaactgcct gtaaagaaaa aagagccatg ctatcaggat gatgaattaa agccccgcgt
    53041 tgaaagtcgc gtcgatggtg ttttctgggt aacgcccaaa gtggataagc agtcaggcga
    53101 aattatccgg cctgagacgt ggttatgttc tccgcttgaa ctactgggaa cggggacgat
    53161 cggtaaagag cattaccgcg tgatgcgctg gaaaaaatta gcaaaccatg aagtcatcac
    53221 aatggcgatc ccgtgtggtg gcattggcga ccgtgacggc tggcggttgc ttaaagatca
    53281 cgggctgaat gtgacaacaa acggcaaata cagggctatc ctggctgact ggatgcagtt
    53341 aagcggaagc catgaggaat ggcagttaag cacaacaacg ggctggcatt ttggcgcgta
    53401 catcatgccg gacggttcaa tcattggtga ttctgaaaaa ccgatcctgt ttaccggaaa
    53461 aagtgccgct gttaatggct attcggttgc gggtacggcg gagggctggc gtgacagcgt
    53521 ggcgcggctg gctggtggca atccgtccat gatgctgggt gttgccacgt cactggcagc
    53581 acctttgatt ggccttgttg gtgctgacgg cttcggggta catcttttcg aacagtcatc
    53641 ggcagggaaa accaccacgc agaacatcgc atcaagttta tggggagagc cggacgcaca
    53701 acggcttact tggtacggaa cggcgttagg tatcgccaac gaggcagagg cacacaacga
    53761 cgggctgtta cccctggatg aaataggtca ggccggaaac gcgcgggagg tgtccacgtc
    53821 agcctatacg ttgtttaacg gctccgggaa attacagggg gcgaaggacg gcggcaaccg
    53881 ggagataaaa cactggcgca cggtggcaat cagcaccgga gaaatggacg ttgagacatt
    53941 cctcaaaacg gaggggataa aagtcaaagc ggggcagctt gtccgcctgc ttaacgttcc
    54001 gatggaaaaa gccacgcaat ttcacgaata cagcaccgga aaggcgcacg cagacgcgtt
    54061 aaaagatgcc tggacagaaa atcacggggc ggcgggtcgt gaatgggtta aatggctggc
    54121 agaccaccag caggaggcaa aggatacggt aagggaatgc cgcgaacggt ggcgcaacct
    54181 gataccggat agctacggcg agcaggtcca ccgcgtgggg gagcgtttcg ccatactgga
    54241 ggctgcgctt gtgctttccg gtcatgtaac tggctgggcc gcgcaggaat gccgggacgc
    54301 aatacagcat aactttaatg cctgggtgaa ggagttcggc acgggtaaca gggaatttaa
    54361 acagatggtt gaacaggctg aggcgttttt gtcgtcgttc gggttcagtc gataccttcc
    54421 ttacccaaac agtgatgagc gtgatttacc gattaaagac cttgccggat acagaaaggg
    54481 gagtatcaga aatgaagatg atgagttccg tttttacacg tttcctcatg tgtttgaggg
    54541 tgagatagca cagggattta atccgtctca ctttgcccgc gcgttgagtg ctgccggaat
    54601 gctggaagcg ggtaacgatc gccgttacaa gaaaaaggcg ctcggcaaaa ttgggggtaa
    54661 gcagcatgtt ttttacgtga tgatgttcaa gcctgaggca gaagattaac tcccctgtgt
    54721 gaggtgaaaa gttgcgggtt atgcgggtta tcctgtgtat aagtgaatta actgtatgaa
    54781 taaaaaggaa atcaataacc cgcacgtaac ccgcaaaatg gcagttataa cccgcaaaag
    54841 tgcgattata acccgcagaa tttcaccgta agggtgtaaa caagcgtaac tatcaacgtt
    54901 aaaaccggag aacagacagc atgacagcac agatagcggc ttacgggcgg ctggtggctg
    54961 acccgcagtt aaagaccacc agcaagggca cacaaatggc tatggcaagc atggcggtcc
    55021 ccctgccgtg caatcaggca gatgacggac aggcgacgat gtggttatcc gtcctggcgt
    55081 ttggcagaca ggccgacgca ctggcaaaac accacaaagg cgagctggtg agcgtggcgg
    55141 gtaacatgca ggtaagccag tggacaggcc agaacgggga aacgcggcag ggctggcagg
    55201 ttatcgcaga cagcgtaatc agtgccagaa cggcgcgacc tagtggcaaa aaaggccagc
    55261 agggtcaggc tactgacgct ctaaataggg ctaagcaaca ggcggggaat gatgatccat
    55321 acggggataa tatacccttt tagcgataac agggggatac atgccagtga catttgaaga
    55381 agttcagcaa cataaaaagt ttcatggtat tgatgatctg gaaaccacga cagcaaaaaa
    55441 atatcgccgt ctgctttctt ccgatgcgtt atttgttgtg gatcatcatg attttctgcg
    55501 cagctcactg accggggaaa ttttcgcaac caaccgtgag caggtggaag cgatgatcga
    55561 atatttgtgg aaaataaggc gcagaatgcg cgatcccgtg aaacagtaaa gcgataaagg
    55621 cccggatttt tcccgggtct tttttcaggt tttgtaaatt atttgttcgc ggttgttcca
    55681 gattgttcgg tgattctggc tgatgtttac atactgattt ttatgtatat gttggcgtgt
    55741 ggcactcaga cgtgagccgc cacaatgccg cctgaccccc tgcgcgatgc cgggttgatc
    55801 tgcgagatgc cgagagtgtc tggcggcgct ccctccgtgt tggtttcacg tcctgaatct
    55861 taaccaatac gagaaaacct tcatgaagaa attaatcgaa ctccgccagc aaaaaaacgc
    55921 cctgaaaaac cagatgcgat ccctgctgga aaaagccgac agtgaaaacc gcagcctgaa
    55981 cgatgacgag ggcaaacagt ttgatgaact gcgtgcaaaa gccgattccc tcgacacaga
    56041 aatttcccgc ctcgagtctg tggctgatga agaacgcagc aagccaggaa cgggcatcca
    56101 gaaattatca tctgatgaat tgcgtaacta catcgtaacc ggagatgtgc gatcactgtc
    56161 caccagcact gacagcggca gggatggcgg atataccgta attcctgagc ttgatcgcga
    56221 agtcatgcgc cagctacagg atgacagtgt tatgcgcgtg atcgcgaccg tgaagaccgc
    56281 aaaatcaaat gagtttcaga aactggtttc cactggcggc gcaactgtag gacgaggtac
    56341 agaaggcagc gcacgcagtg aaaccaacac cccgaaaatt gaacgcgtaa ccatcaagct
    56401 gaatccgatc tacgcctacc cgaaaaccac gcaggaaatc cttgattttt cagaggtgga
    56461 tattctgggc tggttatcct ccgaaattgc cgacacgttc gccagcaccg aagaggatga
    56521 ttttgttaat ggcgacggta acggcaagcc gaaaggattc atggcttaca cccgtgcggc
    56581 gaccagtgac aaaacccgcg cttttggcac cattgaaaaa atggtagcgg caagtggaac
    56641 cgccattaca gcggacgaac tgatcgacat tctctacaag ctgaaagcga aataccgcaa
    56701 aaatgccgtc tgggtgatga actcgggcac ggcagggaca ctacagaagc tgaaaaatga
    56761 gaacggcgat tatatctggc gcgacagcct taaagaaggt gcgccggata tgttgcttgg
    56821 tcgtcctgtt tactgcctgg agtccatgcc ggacatcggc gcaggaaaag caccgctggc
    56881 ggttggcgat ttcagtcgtg gttatttcat cgttgatcat gtaacgggta ttcgtaccag
    56941 accggacaac attactgaac ccggattcta caaggtccat acggataaat atctgggcgg
    57001 tggtgtggtg gattcaaacg ccatcaaaat tctggaaatg aaagctggct agtcatgagt
    57061 aaggaggagg ctgcggcctc ctttttcagc tttatggagt acaccgatga aaaacaccga
    57121 ttttgaaatc cgcacatctg aactgaccgc cagcaataaa aagctggtgg ggtatgccgt
    57181 tcgctggaac tgtctttcag aaattatctg ggatgagttc cgcgaacaat tcacgccggg
    57241 ggcttttgct gactatctgg ctgcgggtaa tgatgtgcgc tgcctgtatg agcatgacta
    57301 tacccgactg ctggggcgca ccaaatccgg cactctggta ctgactgagg ataacaccgg
    57361 gttacgtttt gagctgacac cgccggatac ccagcttggg aaagatgtgc ttacgctggt
    57421 ggagcgaggc gacattacag gaatgagctt tggctttcgc gcattatgcg aggagtggaa
    57481 tatcgcgcaa aaaccgtatc tgcgtactgt taccgccgca gaactcaggg aaatcacgat
    57541 aacgtcgatg cctgcttacc ccgaatctgg tgtggagatt gcccaccgtt cgttgtttgc
    57601 acagcaccct gaattatgcc cgacaggaaa taatcgtcat cgctgggctg aactggcggg
    57661 gttgtgatat gtggtggcct tttagtcgta aaaaaagcga gcagcgtaac ctgtccattg
    57721 atgattttct ggcgctgtcc ggcgtaccga ataccggatc cggagaatat gtttctgccg
    57781 ggacggctga atcattgcct gcagtgatga acgcggtttc tgtcatcgct gaggcggtgg
    57841 ccacgatgcc gtgttatctg tatctggtac gtaatgacaa ggacagggag gcgcgggaat
    57901 ggctggacag tcacccggta gatattctgc tgaatgagca gcctaattcg tgccagacac
    57961 cttaccagtt taaacgcaca atgatgcgtc actgcctgct gaacggtaac gcctatgcgg
    58021 ttattgagtg ggggcgggac ggacagccaa aatcacttca tccttatgct ccgggttgtg
    58081 ttgtaccgga acgcacaggc gcacacaaat accgctatac catcaccgaa ccctatacag
    58141 gaacggtgcg cacgtattta caggaagaag tcctgcatct tcgctatgcc tcggatgatg
    58201 gttttctggg acgttccccc gtcacgattt gccgtgaggc gctggggctt ggccttgctc
    58261 aacagcgcca cggagccagc attatgaaag atggcatgat ggcggcaggg attatcacgt
    58321 caggtgaatg gctggacggc gtgaaaggta aacaggcatt agacgcactg gaacgctaca
    58381 agggggcgaa aaatgccgga aaaacgccaa tccttgaagg gggcatggat tacaggcaac
    58441 tgggaatgag taaccaggat gcggaatggc tggcctcccg tcgcttctcc attgaagaca
    58501 tcgccagaat gttcaacgta tcgcctattt ttctgcagga atacagcaac agcacctaca
    58561 gcaatttcag tgaggcaagc cgcgcgtttc tgaccatgac aatgcgcccg tggctggcga
    58621 acttcgaaca gcaaatcaag gccgctttgc tggtggcttc tcccgtacct ggtacccgtt
    58681 atctggttga gtttgattca gccgatttat tacgcgccac acccaccgaa cgttatgcca
    58741 cgtatgagaa agggattaag aacgggatca tgaatccgaa cgaagcccgt gagcgtgagg
    58801 gtatgccgcc gcgtgaaggt ggtgatgagt tcagccaggc atggaaacag actgtggaaa
    58861 ttaaagggag aaaagatgag tgaagccaga attacacctg atgaagtcag ggcacatctt
    58921 cgacttgatg atgatttatc cggtgaaggc gaacttctga aaatgtatac cgatgcggcg
    58981 ctggaagcct gccagaagca tatcgggaaa cgttttgaag acgggctgga atttaccccg
    59041 gcaatgcgtg ttggttgcct gatgtacatc gctttcctgt acgagaaccg ggaagcggtt
    59101 tcaccagtgg agcactcgga actgcctatg gctatttctg cgctctggtc agtttatcgt
    59161 gacgtagggg tgtactgatg ccgtggcaac cattaaggcg atgcactgag cctggctgta
    59221 ataagcgcgt gaagtccggc aagtgtgaag agcacaggcg ggctgcatgg cgtgcagagg
    59281 atgccagacg gggacaccgc cgcgcgcgcg ggtattcccg acagtgggac aaataccgcg
    59341 ccctgtactt gagcaaaaac ccgttatgcg tgcgttgtct ggctaagggg atttatacgc
    59401 cagctcttgt ggtggatcac atcattccca tcaatggcgg cggtgatgtt ctcttctggc
    59461 ctgagtggaa tcaccaggca ttgtgccaga cgtgccacaa ccgtaagacg acacgggaag
    59521 atccagccac gaaagcgaac cgtaaggcgg gcatgtatcg cgagcaggaa gaacgggcag
    59581 cacaccgtaa cgactggatg tatggcgatg atgactgaac aggagcaaac caggctgata
    59641 cgtggactga taaggcagcg tgacacatgg aagacacagg agacagagca caaagccaac
    59701 aggacagggc gcacaaaacg caccacagcg aagcgattaa ccgaccgtga ccgcgaggtc
    59761 atggaatgtt ttcgcaatcg ctggtgaggc cgtcagaggg ggtgggggtg gttttcagga
    59821 cgaaaccgtc cctgccggac accgaccgcc ccctcaaatt tttgtgcacg gtaatttttt
    59881 tgaaaataat tgggcgaaaa aagaacatgg caagaccacc aaaagccccc gcttacctgg
    59941 atgaaatcgc ggtcaggcag tggaaggaaa aatcgcgcca gctttccggg cgggaagacc
    60001 ttacccccgc cgactggagc aatctggaac tgtattgcgt taactactcc atataccgca
    60061 aagccgtcga agaccttgcg acgcgcgggt tcagcattgt taacagtcag ggcagcgaga
    60121 gcagaaaccc cgccctgagc gcaaaggctg acgcagaaag aataatgatc aaaatggctt
    60181 ctttgctggg ttttgacccg gtaagccgcc gcagaaatcc accggaaaca gaggaagagg
    60241 acgagcttga ctgcctggca tgagtacgca gaaggcgtaa aaaacggcaa aattacggcc
    60301 tgtaaacgac tgaaacaggc cgttaaacgg tatttttctg accttgaaaa ccccctttac
    60361 acgttcgatc cggaggtcgt tgagcggttt attgcctttt ccagggtgtg tccgcacgta
    60421 aaaggcgcaa tgcgtggtag ccccattgag ctggagccgt ggcagcagtt cgcctttgcg
    60481 tgcatcctgg gctttaaggt taaggccacc ggacggcgca aatacaccag cgcattcatt
    60541 gaagtaccgc gaaaaaatgc caaatccacg gtcgccgcta tcctggctaa ctggtttctg
    60601 gttatggaaa acgggcagca ggatatttac accgccgccg tgagtcgtga tcaggcgcgg
    60661 atcgtgtttg atgatgcgcg tcagatgtgc cttttatccc gaccgttacg aaagcgggta
    60721 aatattcagg cgcacaaggt gatacacccg aaaaccaaca gcctgttaaa gccactggca
    60781 gcaaaagcgg caaccattga ggggacaaac ccgagtcttg ccattgtgga tgaatatcac
    60841 ctgcacccag acaacggggt ttattccgca cttgagctgg ggatgggggc gcgtccggag
    60901 gggctgttat ttgccattac cacatcgggg agcaacgttg tttcagcctg taaacaacac
    60961 tatgactatt gctgtcagat actggatggt gaagagatga acgaatccat gttcgttctg
    61021 atttacgagc tggatgatga aagcgaggtt gacgatccgg cgatgtggat aaaggcgaat
    61081 ccaaatatcg atgtttccgt cgatcgtgaa aaactggcct caaccatcca gaaagcgcgg
    61141 ggtattccgt cgcagtgggt ggaaatgctc accaagcgat tcaatatctg gtgtcagggg
    61201 gctacgccgt ggatgggtaa cggtgcatgg acggagtgcg ccggaacgtt cgcggaggcg
    61261 gatttatacg ggcaggagtg ctacgcgggg ctggacttat catcaaccag cgatatttcc
    61321 agcgtgtgct atgcctttcc ggtcggtaaa aagattatgc tggtttcccg tcactatctg
    61381 ccggaatttc agctacagaa ccccgccaat aaaaaccgcg ccatctatcg ccagtgggta
    61441 aaggcgggct ggatacgcac aacaccgggt gactgcattg attatgaccg tatccgtgat
    61501 gacatcatgg cggatgcaga gaatttcaat atcaggctgg tgggcttcga tacatggaac
    61561 gccacgcacc tgaggacgca gctacagggg gcaggattag aagtggagcc gttcccgcaa
    61621 acataccttc gtttcagtcc ggcggcgaaa tcgttcgaag tttttgttaa ccggaaggtg
    61681 attgtgcatc gtggtgatcc ggtgctggcc tggtcaatga gtaatgttgt gatgcagagt
    61741 gacgcgaacg ccaatatcaa gccgaacaag aaaaaatcat ccaacaagat agacccgagc
    61801 gttgcggcgc tgatggcgtt tggcacattc caggcagagc atgaggaatt tgcatttgat
    61861 atgagcgaca gccacaaaaa gcgacttgcg gcgtttgatg gggtatgacg agaatgactg
    61921 aaactgatct actaaaaata attcgccgca ttaccggaat cagtcagcag cctgacgaac
    61981 aggctacaca gccggacagc gtgatagccg aaaattacgc gcgtgtggtg gctgaggtga
    62041 tgcgccgtga cggtattgag cttaacggcg tggatatgcg caacatacga accagagtcc
    62101 ttgagttgct ggcataccgt cgccgttctc aacaacggag ggagagcgcg aaaaatactt
    62161 accagtggaa gaagccggaa cgactgcggc ggtaacttgc tgatattccc gataacgcaa
    62221 aattgcgttg gctggcatag tgcaaatttg cacgatgacc taacccattg acttttcttc
    62281 aatcctcaat tttgaggagg ccggaagata aaaaactgtc aaaacttgtc atgcaaaacg
    62341 ttaccagcac ggctattgat ttattcggcc cctgcaactg ctcccagaat ggtggggatc
    62401 tgaaatccga ccctgaaaaa gtgcgcaaaa gtggtacgca aaaattagcg tccagccaca
    62461 gccaccagct agacagaacg cccagagcat tgtcagtaac tggttgatat ttccgacagc
    62521 cccaaaatgg gttggctggt gggtgagttg cagatttgca actcgaccat gaaactacgg
    62581 aaactacccg tagtttgggt agtaagagta acacccagat tttggggctt acttgcgata
    62641 cccaaatata gggtatcggt ggcgtcactg gccccatttt cctgatgtgt ttgcgggtta
    62701 tttgggggtg gttgcgggtt attttgaatg gcttgcgggt tacgatttgg ctaacatttg
    62761 aacgtgtcat ttttcaacgc attgatatta aagaataaaa aatacttagc acgcaaagat
    62821 aacccgctaa cccgcataac ccgcactgtt ttgtatatat atatacgaaa aattgcattc
    62881 aggaggggga gcaaaatccc taccgtctct tatctcttgg agtgcccgcc tcttcgaatt
    62941 tttacccccg tgaaattaca aaatttccct gactgatagc gagttgcaga tctgcaactc
    63001 gactatgaaa ctacggaaac tacccgtagt ttgggtagta agagtaacac ccagattttg
    63061 gggcttactc gcgataccca aataaagggt atcggtggaa gaaatatcgt ttctcatatg
    63121 tgagtaccga gggcggaatt ccgccttcgg ttacttattg tgctcatgca cagggagggg
    63181 cgggtcaaat ctctgtaccc tgacgtcttc cggactgcca gccccatcga ttttttatac
    63241 ccgcgaaaaa tgaaaaacgc ttcacgctgg taggcctgat gccgatatgg ggagccgcat
    63301 accggagaat atccaccagc acggcaacag cagaatagcc ccgacacaga aaaaccacga
    63361 atatggggtt tttgttatgg catggtcatg atgaccgccc ctgctctgaa tggtggaaat
    63421 caccacgcag atcatccacc agcttcatga cggatagccg gaaaatgata atgattaaag
    63481 cgtgttgatc tttgttcgcg gttgttcgat attgttcgtg aagcggtgta gttaatggtg
    63541 tagttaatat cactgttttt gacgggcttg ttatttgcaa cataatgaaa aataaagact
    63601 aattaactaa atgatataaa tgatagttat ctatcatgtg gagtagattg gtcaggcaaa
    63661 taagctcttg tcagcggcag ggcgttctgc cgataaccgt aaccgaagat gataactgac
    63721 aatgggtaaa acgaacgact ggctggactt tgatcaactg gcggaagaaa aagttcgcga
    63781 cgcgctaaaa ccgccatcta tgtataaagt gatattagtc aatgatgatt acactccgat
    63841 ggagtttgtt attgacgtgt tacaaaaatt cttttcttat gatgtagaac gtgcaacgca
    63901 attgatgctc gctgttcact accaggggaa ggccatttgc ggagtcttta ccgccgaggt
    63961 tgcagaaacc aaagtggcga tggtgaacaa gtacgcgagg gagaatgagc atccattgct
    64021 gtgtacgcta gaaaaagcct gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa
    64081 tcaagaactg gaactcagtt taaatatggc tttcgccaga gcgcgcgagc accgtcatga
    64141 gtttatgacc gtcgagcact tgttactggc gctgctcagt aacccatctg cccgggaggc
    64201 gctggaagcg tgttctgtgg atttggttgc gctccgtcag gaactggaag cctttattga
    64261 acaaaccaca cccgttctgc ctgccagtga agaggagcgc gacacacagc cgacgctgag
    64321 ttttcagcgt gtactgcaac gtgcggtctt ccatgtccaa tcctccggtc gcaatgaggt
    64381 aaccggtgca aacgttctgg tcgctatctt tagcgaacag gagtcgcagg cggcatatct
    64441 gttgcgtaaa catgaagtca gccgtctcga tgtggtgaac tttatctctc atggcacgcg
    64501 taaagacgag ccgacacagt cttctgatcc tggcagccag ccaaacagcg aagaacaagc
    64561 tggtggggag gaacgtatgg agaatttcac gacgaacctg aatcagcttg cgcgcgtggg
    64621 cggaatcgac ccactgattg gtcgtgagaa ggagctggag cgtgctattc aggttctctg
    64681 ccgtcgccgt aaaaacaacc cgctgctggt gggggaatct ggtgtcggta aaaccgcgat
    64741 tgcggaaggt cttgcctggc gaattgttca gggcgatgtg ccggaagtga tggctgactg
    64801 tacgatttac tctctcgata tcggttctct gttagcgggc actaaatatc gcggcgactt
    64861 tgaaaaacgt tttaaagcgt tgctcaagca gctggagcag gacactaaca gcatcctgtt
    64921 tattgatgag atccacacca ttatcggtgc gggggcagcg tctggtggcc aggtcgatgc
    64981 ggctaacctg atcaaaccgt tgctctccag cggtaaaatt cgcgtaattg gttcgacaac
    65041 ctatcaggag ttcagcaaca ttttcgagaa agaccgtgct ctggcgcgtc gcttccagaa
    65101 aattgatatt actgaaccgt cgatcgaaga aactgttcaa atcatcaatg gcctgaaacc
    65161 gaagtatgaa gcgcaccacg acgtgcgtta taccgcaaaa gcggtgcgtg cagcggtaga
    65221 gctggcggtg aaatacatta acgatcgtca tctgccggat aaagccattg acgttatcga
    65281 cgaagcgggc gctcgcgcac gcctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt
    65341 ggcggatatt gagtccgtgg tggcccgtat tgcgcgcatt ccagagaaga gtgtttctca
    65401 gagtgaccgc gataccctga aaaacctcgg cgatcgcctg aaaatgctgg tcttcggtca
    65461 ggataaagcc attgaggcgc tgactgaagc cattaagatg gcgcgtgcag gtttaggtca
    65521 cgaacataaa ccggttggtt cgttcctgtt tgccggccct accggggtcg ggaaaacaga
    65581 ggtgacggta cagctttcga aagcgttggg cattgagctg ctgcgctttg atatgtccga
    65641 gtatatggaa cgccataccg tcagccgtct gattggtgcg cctccgggat acgttggttt
    65701 tgatcaggga ggtttgctga ctgatgcggt catcaagcat ccacatgcgg tgctgttgct
    65761 ggacgaaatc gagaaagcgc atccggacgt gttcaatatt ctgttgcagg tgatggacaa
    65821 cggtacgctg accgataaca acggacgcaa agcggacttc cgtaacgtgg tgctggtgat
    65881 gaccaccaac gccggggtac gtgaaactga gcgtaaatcc attggtctta tccaccagga
    65941 taacagcacc gatgcgatgg aggagatcaa gaagatcttt acaccggaat tccgtaaccg
    66001 tctcgacaac attatctggt ttgatcatct gtcaaccgac gtgatccatc aggtggtgga
    66061 taaattcatc gtcgagttgc aggttcagct ggatcagaaa ggtgtttctc tggaagtgag
    66121 ccaggaagcg cgtaactggc tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc
    66181 gatggcgcgt gtcatccagg acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg
    66241 ttcgctggtg gacggcggtc aggtcaccgt cgcgctggat aaagagaaaa atgagctgac
    66301 ttacggattc cagagtgcac aaaagcacaa ggcggaagca gcgcattaat ctgattgtta
    66361 ggtaggttgg tcaagtccgt aatctcgaaa gaggttacgg actttttgtt tatggggtgg
    66421 aggaggttca gacccttttt ttaatgatga tggtaagttg ttgataatta gtgctgcggg
    66481 aaggtaagga taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg
    66541 ggctaaacgt gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg
    66601 cggtaaaacc tttgcgaacg cgttcactgc cgtacagaca gataaaatgc gaaaaaaaag
    66661 ctcgcacttt cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc
    66721 gatacgttgc cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc
    66781 aaattgtttt gtcgcctgac ctcatgggtg gcaacggggc gctactatag ggagttggag
    66841 taaaacggtc aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaagttg
    66901 tgcagtatat ctacatcgag acaagttacg gacttatact tccaaagtac ttcatacata
    66961 tcacaaaata aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca
    67021 ggcggtaaaa caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg
    67081 tcagttcaac agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac
    67141 cggagatgtg tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag
    67201 gcaacgtttc aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct
    67261 ctggggtatc actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt
    67321 aaagatttgc gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg
    67381 ctaagccgca ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc
    67441 tgacgcgaaa aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca
    67501 attataacac tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca
    67561 tcgtggtacc cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag
    67621 gtaatcccgg cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg
    67681 gcagtcgata agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt
    67741 tttagacgca ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc
    67801 cacgccgtac ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg
    67861 gccgagttcg tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc
    67921 ttcgcgatcg ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg
    67981 atagggcgag cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca
    68041 tagcaccgcg cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg
    68101 aaaaatacca cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc
    68161 cagcaggccg ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg
    68221 agaaagctga accaggcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata
    68281 gcttacaaac cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ctctgcgtga
    68341 gtaccttgct caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt
    68401 ccgcgaagtc gatgggtgac cattaacacc gttttctcac gcatcatttc tgcaagcaat
    68461 tcaaggatct ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc
    68521 agtggcgcat catgtaacag cgcacgggcg atagccagac ggcgcagttc accaccggag
    68581 agctggcgtc cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc
    68641 aggccaacgc gacgcaagct ctccgccaga gcctcatcac tactgccagg cgaggcgagt
    68701 aacagattat cacgcagcgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg
    68761 gtctgtcgta gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg
    68821 ccctgttgcg ggtcccaagc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg
    68881 gttcgcccga gaatcgcaat atgttccccg gcgtttacct gaagagaaat ccctttaagt
    68941 gcctgttggg attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga
    69001 tcggcaacac gagtttgggt atcagggaag gtgacttccg gtttttgatc cgttaaatca
    69061 gtgatccgaa cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt
    69121 gccagtgctt caaacgcggc taacgcgcag aagacaaaca gggcaattaa cgcgccgggt
    69181 tgagcattgc cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg
    69241 ccaatgagta gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct
    69301 tccagccatt gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa
    69361 atggtcagct cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga
    69421 agatgagtca gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg
    69481 aaaagcgtca gtaacataat gccgcccagc gtaaaggcga gggtgaaatc aaggaaactt
    69541 aacccgattg tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc
    69601 agataaagat gatcgagcgt atcaacatcc gccaccacgc gattgagcaa ttcgccctga
    69661 cgatagcgcg ccagtccggc aggggagagg ggcagcaatt tgctgaaggt gtaaatgcgc
    69721 agatgctgca acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg
    69781 gcagtacggg tgattgctgc gccacgcacg cccgcagcgg gtagcatata gttgaagctg
    69841 tacagtccgg caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac
    69901 agaccgatac tggcgagcag cgtgacaatt gccagcacga taccaagact taacatccat
    69961 ttatgacgtt tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga
    70021 cgatgggcca gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct
    70081 tgctcaataa tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct
    70141 aactggtggg tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt
    70201 acgcgctgtt cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa
    70261 cagggattta gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg
    70321 gcagcctggt cgccaacagg cgtatcaacg ccttgtggca ggagcggtag aaactcgctg
    70381 acccaggcgt tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt
    70441 agtacgttat cccgcaatgt tgctgccggt aattgtgggt tttgcccaac ccaggagaga
    70501 tgtttacgcc atgattctgg tgataaatcg cgtaattcta tcccgttgat tcgtaatgat
    70561 ccctgatatg aaagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg
    70621 cgaccaacca acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc
    70681 gttttacctt ccggcgacgt gataaacaga tcctcggcct caatggtcac cggatcggtc
    70741 gatgctaatt ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc
    70801 agactgtcag ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgc
    70861 aatggctgga aaaactctgg cgcaaggatc agggccagaa aacccgcagc cagcgtcaca
    70921 ccggtatcgt agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc
    70981 accagagcaa ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagc
    71041 cgtagcactt ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt
    71101 tcagcttcac cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa
    71161 tgcccactta agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatc
    71221 ccaaccagcg ccataaacaa cggaattaac ggtgctgtgc ccagcagaat gagcgccgca
    71281 gcccagttag aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc
    71341 atttgcggca ggtagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccaac
    71401 gtcgcccagc tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg
    71461 tcgagaacct gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt
    71521 tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca gaaccagtaa cgtaaaggga
    71581 agcagcaggg cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg
    71641 aaccaggcct gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc
    71701 agccaacgtt gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacgg
    71761 gatttattca ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac
    71821 aacggggaaa aaataaaggc gacccatagt cgcatggtgt caccttcttt acttttgtta
    71881 ctgatttgta aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg
    71941 ctgccatgca gcctgtaccg gccgaagtaa tggcctgacg ataaatatga tccatcacgt
    72001 cacccgcggc aaagacgcca gggatgctgg tctgggtggc attaccatga atacccgact
    72061 gcactttgat gtagccgttt tccagttcca gctgcccttc gaaaatggca gtattcgggc
    72121 tgtgaccgat agcaacaaac agaccagcaa cgtcgagtga ctcgatgtta tcgctgtttt
    72181 gcgtatcgcg cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg
    72241 tacggttggt gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa
    72301 ggattttttc cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt
    72361 tagacagata cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct
    72421 ggttgcgata gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg
    72481 cttcttcaga gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt
    72541 cgcaagtgta ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct
    72601 tgttgatatg atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgct
    72661 ccattaataa cggaccggtc agatcgtttg gatcgccagg ccagttttcc acttccgtgg
    72721 tggtggtcag ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc
    72781 gcgccgcgta gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac
    72841 tgtgtttggt cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta
    72901 gggaatttac agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc
    72961 agcatcgata agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg
    73021 ctttataagc cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt
    73081 actaaaaatc gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa
    73141 gaaaaaaaac agtattctta tatgcgcata accatgcatg taaataccat gtttaccgtg
    73201 ctagtgaaat ctacgtatgg cgtggacaga cgccattcgt gatgtcgata gctgccacaa
    73261 ggcaacggtc ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc atgatttcgg
    73321 tctatcgtga cgggtagcga ctctgaacag tgatgtttca gggtcagaca ggagtaggga
    73381 aggaatacag agagacaata ataatggtag atagcaagaa gcgccctggc aaagatctcg
    73441 accgtatcga tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg
    73501 agctttctaa acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg
    73561 aaagacaagg gtttattcag ggctatacgg cgctgcttaa cccccattat ctggatgcat
    73621 cacttctggt attcgttgag attactctga atcgtggcgc accggatgtg tttgaacaat
    73681 tcaataccgc tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta tctggtgatt
    73741 tcgactacct gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg
    73801 aaaccctgct gcgtctgcct ggcgtcaatg acacacggac atacgttgtt atggaagaag
    73861 tcaagcagag taatcgtctg gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg
    73921 cgtattttga ttacactcct gttaatccat acagcaacag tactggggta acctggtact
    73981 gttgtccgtt ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt tcttgagcca
    74041 ggaatacact gaagacaaag aagtcacatt gacaaagtta agtagcggcc gccgccttct
    74101 ggaggcgttg ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag
    74161 ctttaaccct tcggacccca gctggtcgca aacggcctgg catgaaccta tccataattt
    74221 aggtgggatg cccggtgcgt ggttggcaga tacgctgttc tttatttttg gcgtgatggc
    74281 ttacaccatt cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag
    74341 cgacgaatac attgattatt ttgccgtttc gctacgcatc attggcgttt tggcgctcat
    74401 ccttacctcc tgtggtctgg cggcaatcaa cgctgacgat atctggtatt ttgcctccgg
    74461 tggcgtcatt ggcagcttac taagtactac gctacaacca ctgctacaca gtagcggggg
    74521 aactattgcg ctgctctgcg tttgggctgc gggcctgacg ctgttcaccg gttggtcatg
    74581 ggtgaccatt gctgaaaaac tcggcggctg gattttaaac attctcacct tcgccagtaa
    74641 tcgtacccgt cgcgatgata cctgggtcga tgaagatgaa tatgaagatg acgaagagta
    74701 tgaagatgaa aaccacggca aacagcatga atcacgccgt gcccgtattc ttcgcggcgc
    74761 gctagcgcgt cgtaaacggt tggcggaaaa attcattaat ccgatggggc ggcaaacaga
    74821 cgctgcgttg ttctccggta agcggatgga tgatgacgaa gagattacct acactgcacg
    74881 cggtgtggct gccgacccgg acgacgtcct attttcgggc aatcgtgcaa cgcagccaga
    74941 atatgacgaa tacgatccat tattaaacgg tgcgccaatt accgaacctg tcgctgtagc
    75001 agctgctgct accacggcga cacaaagctg ggctgcgccg gttgaacctg tgactcagac
    75061 gccgcctgtt gcctctgttg atgttccacc ctcgcaacct acagtagcct ggcagcctgt
    75121 accgggtcca caaacgggag agccggttat tgctcctgca ccggaaggtt acccacagca
    75181 gtcacaatat gcgcagcctg cagtgcaata taatgagccg ctgcaacaac cagtacagcc
    75241 gcagcagccg tattatgcac ctgcagctga acaacctgcg caacaacctt actacgctcc
    75301 tgcggcggaa caacctgttc agcaaccgta ttacgcaact gcgccggaac agcctgcaca
    75361 acagccgtat tacgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga
    75421 agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca
    75481 accagccgct caggagccgt tgtaccaaca gccgcaatcc gttgaacagc agcctgttgt
    75541 ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt actattttga
    75601 agaagtggaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat
    75661 tccagaaccg gttaaagaac cagaaccgat caaatcttcg ttgaaagcgc cttctgttgc
    75721 agcagtacct ccagtagaag ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa
    75781 agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa
    75841 tagcggtgga ccgcgtccgc aagtcaaaga ggggattggc ccgcagttgc cacgaccgaa
    75901 acgtatccgc gtgccaactc gtcgtgaact ggcgtcttac ggtattaagc tgccctcaca
    75961 gcgtgcggcg gaagagaaag cccgtgaagc ccagcgcaat cagtatgatt ctggcgatca
    76021 gtataacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca
    76081 gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa acgcagaaga
    76141 tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg
    76201 ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc
    76261 gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ttgtttacgc caattgttga
    76321 acctgtacag cagccgcaac aaccggttgc accgcagcag cagtatcagc agccgcaaca
    76381 accagttccg ccacagcagc aatatcagca gccgcaacag ccggttgcgc cgcagccgca
    76441 gtatcagcag ccacaacagc aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc
    76501 ggttgcgcca cagccgcaat atcagcagcc gcaacaacca gttgcgccgc agccgcagta
    76561 tcaacagccg caacaaccgg ttgcaccgca gcagcaggat accctgcttc atccgctgtt
    76621 gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc cttctctgga
    76681 tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc ttgaacaaat
    76741 ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg tcgtcaatta
    76801 ctctccgggg ccggttatca ctcgctttga acttaacctg gctcctggcg taaaagcggc
    76861 gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt
    76921 cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccaaata aaaaacgaca
    76981 aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc cgtcgccatt
    77041 aacagtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc tggcgaaaat
    77101 gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat
    77161 gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca tgatcgaccc
    77221 gaaaatgctg gagctttcgg tttatgaagg cattccgcat ctgttaacgg aagtcgttac
    77281 tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg agcgtcgtta
    77341 taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga
    77401 agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg acagtatgga
    77461 tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggttg acgaatttgc
    77521 cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa
    77581 agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg ttgatgttat
    77641 tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat ccagtaagat
    77701 tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggga tgggggatat
    77761 gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga
    77821 tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga
    77881 tggcatcact tccgacagtg aaagcgaagg tggcgcgggc gatttcgatg gcgctgaaga
    77941 actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat
    78001 ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca
    78061 gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg aagtgctggc
    78121 cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg tcgcatccgg
    78181 cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta ttagcctgga
    78241 atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa
    78301 aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt tgggctgatg
    78361 ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc agcttcacac
    78421 aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga
    78481 aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt ctggtttctg
    78541 acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca acctggctga
    78601 aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc agcgactggc
    78661 agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa gccagcaatg
    78721 gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat cagtttagcg
    78781 cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa aatggggctg
    78841 tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta
    78901 agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac ttttcaacct
    78961 ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggccagca acatttgctg
    79021 gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttacattc tatgatcctc
    79081 tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac
    79141 gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagaaattcg cgaggcgatc
    79201 gagcgcgccc ggcaaaatcg caatgcaggt cgccgcacta ttctttttgt tgatgaagtt
    79261 caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga cggcaccatc
    79321 acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc actgctttcc
    79381 cgtgcccgtg tctatctatt gaaatccctg agtacagagg atattgagca agtactaact
    79441 caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct gccagatgaa
    79501 acacgacgcg ccattgctga actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg
    79561 gaaatgatgg cggatatggc cgaagtcgat gatagcggta agcgggtcct gaagcctgaa
    79621 ttactgaccg aaatcgcagg tgaacgtagc gcccgctttg ataacaaagg cgatcgcttt
    79681 tacgatctga tttccgcact gcataagtcg gtacgtggta gcgcacccga tgcggcgctc
    79741 tactggtatg cgcgaattat taccgccggt ggcgatccgt tatatgtcgc gcgtcgttgc
    79801 ctggcaattg cttccgaaga tgtcggcaat gccgacccac gggcgatgca ggtggcaatt
    79861 gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag
    79921 gcgattgttt acctagcctg cgcgccaaaa agcaacgctg tctacactgc gtttaaagcc
    79981 gcgctggcgg atgctcgcga acgtccggat tatgacgtgc cggttcattt gcgtaatgcg
    80041 ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc tcatgatgaa
    80101 gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca aacacgctat
    80161 tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc ctggctggct
    80221 gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg cggtaatgtt
    80281 gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt cgataagcac
    80341 aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa
    80401 ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga agagcgtcgt
    80461 aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg atcgaaatcc
    80521 attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga agtgaacaaa
    80581 ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc tgaaattcgc
    80641 gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg taaagacgaa
    80701 aatgacaacg tcgaagtcag ccgctggggc accccgcgtg agtttgactt tgaagttcgt
    80761 gaccatgtga cgctgggtga aatgcactct ggcctcgact ttgcagccgc agttaagctg
    80821 accggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg
    80881 cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta tgttccgtac
    80941 ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg
    81001 ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct gatcccaacg
    81061 gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga tgatctgcca
    81121 attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata tggtcgtgac
    81181 acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc
    81241 ccggaagact caatggcggc gctggaagag atgactggtc atgcagaaaa agtcctgcag
    81301 ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg ctttggcgct
    81361 tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg tgagatctct
    81421 tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg tcgcagcaag
    81481 tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc tgttggtcgt
    81541 acgctggttg cggttatgga aaactatcag caggctgatg gtcgtattga agtaccagaa
    81601 gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat ttttctgaat
    81661 ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga taccgaacaa taattactcc
    81721 tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga ttttcaaatt
    81781 attcgatgta tacaagccta tatagcgaac tgctatagaa ataattacac aatacggttt
    81841 gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga aaatccctga
    81901 tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag cgatcggcgg
    81961 cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc acgctgtcga
    82021 tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg agcgcctgta cagttaactg
    82081 tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac
    82141 ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct gtctgcgtgg
    82201 gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg aaatatccga tgaaacgagt
    82261 cggggcgcgc ggtgaaggca aattcgagcg cattagctgg gaagaagcct acgacatcat
    82321 cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gagtctatct atctgaacta
    82381 tggcaccggt acgctgggcg gcaccatgac ccgctcctgg ccgccgggaa ataccctggt
    82441 cgcgcgcctg atgaactgct gcggcggtta tcttaaccat tacggtgact actcttctgc
    82501 gcaaattgcg gaaggtttga actataccta cggcggctgg gcagatggta acagcccgtc
    82561 ggatatcgaa aacagtaaat tggtagtgct gtttggtaat aacccaggcg aaacgcgaat
    82621 gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta atgcccgcat
    82681 gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg agtggatccc
    82741 tattcgtcca ggaacagatg ccgcactggt taacggtctg gcgtatgtca tgatcaccga
    82801 aaacctggtg gatcaggcat tccttgataa atattgcgtt ggctacgatg agaaaaccct
    82861 gcccgctagc gcgccgaaaa atggccacta taaagcttat attctgggtg aagggccaga
    82921 tggcgtggct aaaacaccgc aatgggcctc acaaatcacc ggtgttccgg cagacaaaat
    82981 catcaaactg gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc agggatgggg
    83041 cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc tggcgattct
    83101 gaccggtaac gttggtatta acggaggcaa cagcggcgcg cgtgaaggtt catacagctt
    83161 accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt cgatgtttat
    83221 gtggaccgat gccattgaac gtggcccgga aatgacagcg ctgcgtgatg gtgtacgcgg
    83281 gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta actgcctgat
    83341 taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata agaagtgcga
    83401 gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg acatcctgct
    83461 gcctgactgc accgcttccg aacagatgga ctttgcactg gatgcatcct gcgggaatat
    83521 gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta agaccatcta
    83581 tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta ctgaaggccg
    83641 tacacaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga ttcctgaact
    83701 gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc cacaagggca
    83761 ccacgttgct tataaagctt tccgtgaaga tcctcaggca aatccactga ccacgccatc
    83821 gggcaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct gggaattgcc
    83881 agaaggcgat gtaatcgatc cactgccgat ctacacgccg ggctttgaaa gttatcagga
    83941 tccgttgaac aaacagtatc cgctgcagct tacgggtttc cactataaat ctcgcgttca
    84001 ctcaacttac ggcaacgttg atgtgctgaa agcagcttgc cgtcaggaaa tgtggatcaa
    84061 cccgcttgat gcgcaaaaac gcggtatcaa caacggcgat aaagtcagga tctttaacga
    84121 tcgtggtgag gttcatattg aggcgaaagt gacgccacga atgatgccgg gtgtggtcgc
    84181 actgggtgaa ggtgcctggt atgacccgga tacaaaacgt gtcgataagg gcggttgtat
    84241 caacgtactg accacccaac gtccgtctcc tctcgctaag gggaatccgt cacatacaaa
    84301 ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat ggatttttta
    84361 ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcctgtaaa gactacaaag
    84421 atttgacgcc agaagtcagc ttccgccgca tttatgaata tgctggcggc gactggcagg
    84481 aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt tcatgtaacc
    84541 actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag
    84601 atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc catatggctt
    84661 gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt
    84721 gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt ccgctgcggg
    84781 cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg
    84841 cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc aatgccaata
    84901 gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg tgagatggga
    84961 agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg tgtagcaggt
    85021 ggttttatcg ttctggcttt ggcgctgctc aaaggcgacc tgcgagcaga agcccagcag
    85081 cgtgttatcg cctgcatgtt tggtttatgg gtgctgatgg gcattggctt tatcgcctct
    85141 atgctccatc ttggttcacc aatgcgcgct tttaactcgc tcaaccgggt aggggcttca
    85201 gcactcagta acgaaatcgc cagcggttcg atcttttttg ccgtaggcgg catcggctgg
    85261 ttgctggcaa tgctgaaaaa gctgtcaccg gcattgcgta cgctgtggct gatagtgacg
    85321 atggttcttg gcgtcatctt tgtctggatg atggtgcgtg tgtataacag cattgatacc
    85381 gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat gtttatgggc
    85441 ggcccgttac tgggttacct gttgttgagt ctggctggcg tcgatggctg ggcgatgcgt
    85501 ctgctgccag caatttctgt actggcactg gtagtgagtg gcgtggtgtc agtgatgcag
    85561 ggcgcagagc tggcgactat tcatagttct gtgcagcagg ccgcagcgct ggtgccggac
    85621 tatggtgcgc tgatgtcctg gcggatcgtg cttttggccg ttgccctgtg cttgtggatt
    85681 gcaccacagc taaaaggtta tcagcctgcg gttccgctac tatccgtctc attcattctg
    85741 cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat gaccgtgggg
    85801 atggccgtcg caagctaatc agtaacaacc ggggtttcgg ccccggttct cttttatttc
    85861 tgcttcgtta acgtgtcata actggtcatc aaattacgat aatcaggaat gtggttagag
    85921 aacaacgtcg ccagtccttc aatatcatta cgccagtcgc gatgcagctc gcacgccacg
    85981 ccgaaccatg tcatcaattg cgcaccagct tgcgacatgc gatcccatgc cgaatgccgg
    86041 gtaatttcat taaaggtccc tgacgcgtca gtcacgacaa agacgtcaaa cccttcttca
    86101 atggctgaaa gcgccgggaa tgccacgcaa acttcggtta ccacaccggc aataattaac
    86161 tgttttttac ctgtcgcttt gacagctttt acaaaatctt cgttatccca ggcgttaata
    86221 tttcccgggc gagcaatgta aggtgtatcg gggaattgtg ctttcagttc tggaactaat
    86281 gggccgttag gaccggtttc aaaactggtg gtaagaatag ttggtaagtt gaaatactta
    86341 gccaggtcac ccagcgccag cacgttattt ttaaacttat caggttcgat atcccgtaca
    86401 agggaaagta aaccagcctg gtgatcaaca agcaaaacgg cagcatcatt tttatcaaga
    86461 cgaacatacg gtttggtcat cctcttctcc tttcgagagt cagtggtacg tctgaaatcg
    86521 atcagatgcg atgcattgct ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat
    86581 gaaatttttc gactgaacga agcgtatgaa atttgtgtta gttcaataaa aacaatcaga
    86641 tgagactaat catctcttca gcattagcgt tatgaatgag taatataaat cgataatacc
    86701 gccgctggga tatcgcgtat tttcacccat tgacaatgtt tttggcggtg gcatgatgcg
    86761 catgaaattt gaacttcctc acggttttaa ttcatgtcca cgtatacccg gcctgtcatg
    86821 cttttgctgt ctggcctgct tttgttgact ctggcgattg cggtgttaaa tacactcgtg
    86881 ccgctttggc tcgcccagga acacatgtcc acatggcagg taggcgttgt cagctcatcc
    86941 tattttaccg gcaaccttgt cggtacattg ctgacagggt atgtcattaa acgcattggc
    87001 tttaaccgca gctattatct ggcctccttc atttttgccg ctggctgtgc cggccttggc
    87061 ctgatgattg gtttctggag ctggttggcg tggcgttttg tcgctggcgt cggctgtgcc
    87121 atgatttggg tggttgttga gagcgcgctg atgtgcagtg ggacgtcacg taaccgtggg
    87181 cgtttgcttg ctgcatacat gatggtttat tacgtgggaa cgtttttagg ccagttactg
    87241 gtcagcaagg tctcaaccga gctgatgagc gtattgccgt gggttacagg tttgacattg
    87301 gcagggatct taccgctgtt gtttacgcgt gtgctgaatc agcaggctga aaaccatgat
    87361 tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc ggcttggcgt gaatggctgc
    87421 attatctcag gaatcgttct gggatctcta tatggcctga tgccgttgta cctcaatcac
    87481 aaaggggtga gcaatgccag tattggtttc tggatggcgg tactggtcag tgcgggtatc
    87541 ctcggacaat ggccgattgg acgtctggcg gataagtttg gtcgactgct ggtgttgcgt
    87601 gttcaggtct ttgtcgtcat tctcggcagt atcgcgatgc ttagccaggc ggcgatggcc
    87661 cctgcgttat tcatcctcgg tgccgctggc tttacgctat atccggtggc gatggcctgg
    87721 gcttgtgaga aagttgaaca tcatcaactg gtggcgatga accaggcctt actgttgagc
    87781 tataccgtgg gaagtctgct tggcccgtca tttaccgcta tgctaatgca gaatttctcc
    87841 gataatttat tgtttatcat gatcgccagc gtatcgttta tctatttgct gatgctgctg
    87901 cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt aaatgaattc aagcagagtg
    87961 tgaacttact gtttcacact ctgctttttt gtttcttcta tctgacttgc tttattccaa
    88021 attttattcg tttaaaaata aaatgtgcag caggttataa ttttgcattt cgctatttcc
    88081 gcacttctta tttgccgcgc ataatcccac gttttacaga tgccccttta attttggcga
    88141 aggatttgtc tatggctggg aatgttcagg aaaaacagct gcgatggtac aacattgcgc
    88201 tgatgtcttt tatcactgtc tggggttttg gcaacgttgt taataactat gccaaccagg
    88261 ggctggtggt tgttttttca tgggtgttta tctttgcact ctatttcaca ccttatgcgc
    88321 taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga
    88381 ttaaacacac gatgggaccc ggactggctt atctcgccgc gtggacctac tgggtggtgc
    88441 atattcccta tctggcacaa aaaccccagg caattctgat tgcgctcggt tgggcgatga
    88501 aaggcgacgg ttcgctaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg
    88561 tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg
    88621 gttctgtggc agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa
    88681 ccgcgcctgc gattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca
    88741 ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg
    88801 gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa
    88861 aagggatgct atgcctggcg gtgatggttg cggtttgtgc cattctgggc tcactggcga
    88921 tggggatgat gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcaatatt
    88981 acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg
    89041 ccattgcgaa taccctggga caagtagctg cgctggtatt ctcgattgat gccccgctta
    89101 aagtgctatt aggtgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg
    89161 cttctggtac gcccgttaat ggctattttc tgaccctggt gctggtggca attctgatta
    89221 tgctgccgac tctcggcatc ggtgatatga acaatctcta taaatggctg ttgaatctta
    89281 actcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagtcgttc
    89341 gcctggcgca gaaatataaa ccagaatatg tctttattcg taataagcct ctggcaatga
    89401 ccgtcgggat ttggtgtttt gcctttaccg cctttgcctg tttgacgggg atcttcccga
    89461 aaatggaagc cttcactgca gagtggacct tccagttggc gctgaatgtt gcaacgccgt
    89521 ttgt
//
LOCUS       DAFATA010000007        85083 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFATA010000007 DAFATA010000000
VERSION     DAFATA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.007"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..666
                     /gene="glpC"
                     /locus_tag="LGJ20_000741"
     CDS             <1..666
                     /gene="glpC"
                     /locus_tag="LGJ20_000741"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBK2384860.1"
                     /translation="PQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQA
                     ITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWRK
                     LDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAGT
                     YGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLAQ
                     ALA"
     gene            860..1762
                     /locus_tag="LGJ20_000742"
     CDS             860..1762
                     /locus_tag="LGJ20_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBK2384861.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETAGDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(1803..2273)
                     /locus_tag="LGJ20_000743"
     CDS             complement(1803..2273)
                     /locus_tag="LGJ20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBK2384862.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(2328..3530)
                     /locus_tag="LGJ20_000744"
     CDS             complement(2328..3530)
                     /locus_tag="LGJ20_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBK2384863.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(3630..4172)
                     /locus_tag="LGJ20_000745"
     CDS             complement(3630..4172)
                     /locus_tag="LGJ20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384864.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            4451..4876
                     /gene="nudI"
                     /locus_tag="LGJ20_000746"
     CDS             4451..4876
                     /gene="nudI"
                     /locus_tag="LGJ20_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBK2384865.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(4915..5517)
                     /locus_tag="LGJ20_000747"
     CDS             complement(4915..5517)
                     /locus_tag="LGJ20_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK2384866.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            5825..6964
                     /gene="arnB"
                     /locus_tag="LGJ20_000748"
     CDS             5825..6964
                     /gene="arnB"
                     /locus_tag="LGJ20_000748"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBK2384867.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            6968..7936
                     /gene="arnC"
                     /locus_tag="LGJ20_000749"
     CDS             6968..7936
                     /gene="arnC"
                     /locus_tag="LGJ20_000749"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBK2384868.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            7936..9918
                     /gene="arnA"
                     /locus_tag="LGJ20_000750"
     CDS             7936..9918
                     /gene="arnA"
                     /locus_tag="LGJ20_000750"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBK2384869.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            9915..10805
                     /locus_tag="LGJ20_000751"
     CDS             9915..10805
                     /locus_tag="LGJ20_000751"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBK2384870.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSSNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            10805..12457
                     /gene="arnT"
                     /locus_tag="LGJ20_000752"
     CDS             10805..12457
                     /gene="arnT"
                     /locus_tag="LGJ20_000752"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBK2384871.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            12454..12789
                     /gene="arnE"
                     /locus_tag="LGJ20_000753"
     CDS             12454..12789
                     /gene="arnE"
                     /locus_tag="LGJ20_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBK2384872.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            12789..13175
                     /gene="arnF"
                     /locus_tag="LGJ20_000754"
     CDS             12789..13175
                     /gene="arnF"
                     /locus_tag="LGJ20_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBK2384873.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(13169..13435)
                     /gene="pmrD"
                     /locus_tag="LGJ20_000755"
     CDS             complement(13169..13435)
                     /gene="pmrD"
                     /locus_tag="LGJ20_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBK2384874.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(13545..14900)
                     /gene="menE"
                     /locus_tag="LGJ20_000756"
     CDS             complement(13545..14900)
                     /gene="menE"
                     /locus_tag="LGJ20_000756"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBK2384875.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(14897..15859)
                     /gene="menC"
                     /locus_tag="LGJ20_000757"
     CDS             complement(14897..15859)
                     /gene="menC"
                     /locus_tag="LGJ20_000757"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBK2384876.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(15859..16716)
                     /gene="menB"
                     /locus_tag="LGJ20_000758"
     CDS             complement(15859..16716)
                     /gene="menB"
                     /locus_tag="LGJ20_000758"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBK2384877.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(16731..17489)
                     /gene="menH"
                     /locus_tag="LGJ20_000759"
     CDS             complement(16731..17489)
                     /gene="menH"
                     /locus_tag="LGJ20_000759"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBK2384878.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(17486..19156)
                     /gene="menD"
                     /locus_tag="LGJ20_000760"
     CDS             complement(17486..19156)
                     /gene="menD"
                     /locus_tag="LGJ20_000760"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBK2384879.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(19245..20540)
                     /gene="menF"
                     /locus_tag="LGJ20_000761"
     CDS             complement(19245..20540)
                     /gene="menF"
                     /locus_tag="LGJ20_000761"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBK2384880.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(20619..20924)
                     /gene="elaB"
                     /locus_tag="LGJ20_000762"
     CDS             complement(20619..20924)
                     /gene="elaB"
                     /locus_tag="LGJ20_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBK2384881.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(20979..21440)
                     /locus_tag="LGJ20_000763"
     CDS             complement(20979..21440)
                     /locus_tag="LGJ20_000763"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2384882.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            21505..22422
                     /gene="rbn"
                     /locus_tag="LGJ20_000764"
     CDS             21505..22422
                     /gene="rbn"
                     /locus_tag="LGJ20_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBK2384883.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            22610..23830
                     /gene="elaD"
                     /locus_tag="LGJ20_000765"
     CDS             22610..23830
                     /gene="elaD"
                     /locus_tag="LGJ20_000765"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBK2384884.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHAATN
                     SPLKPAGPDNSEDLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPKAVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSEFMGEMSNNNIKVHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPQNRASGKRVS"
     gene            24241..24847
                     /locus_tag="LGJ20_000766"
                     /pseudo
     CDS             24241..24847
                     /locus_tag="LGJ20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24950..25453
                     /locus_tag="LGJ20_000767"
     CDS             24950..25453
                     /locus_tag="LGJ20_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBK2384885.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(25520..26977)
                     /gene="nuoN"
                     /locus_tag="LGJ20_000768"
     CDS             complement(25520..26977)
                     /gene="nuoN"
                     /locus_tag="LGJ20_000768"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBK2384886.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(26984..28513)
                     /gene="nuoM"
                     /locus_tag="LGJ20_000769"
     CDS             complement(26984..28513)
                     /gene="nuoM"
                     /locus_tag="LGJ20_000769"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBK2384887.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(28677..30518)
                     /gene="nuoL"
                     /locus_tag="LGJ20_000770"
     CDS             complement(28677..30518)
                     /gene="nuoL"
                     /locus_tag="LGJ20_000770"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBK2384888.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(30515..30817)
                     /gene="nuoK"
                     /locus_tag="LGJ20_000771"
     CDS             complement(30515..30817)
                     /gene="nuoK"
                     /locus_tag="LGJ20_000771"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBK2384889.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(30814..31368)
                     /gene="nuoJ"
                     /locus_tag="LGJ20_000772"
     CDS             complement(30814..31368)
                     /gene="nuoJ"
                     /locus_tag="LGJ20_000772"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBK2384890.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(31380..31922)
                     /gene="nuoI"
                     /locus_tag="LGJ20_000773"
     CDS             complement(31380..31922)
                     /gene="nuoI"
                     /locus_tag="LGJ20_000773"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBK2384891.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(31937..32914)
                     /gene="nuoH"
                     /locus_tag="LGJ20_000774"
     CDS             complement(31937..32914)
                     /gene="nuoH"
                     /locus_tag="LGJ20_000774"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBK2384892.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(32911..35637)
                     /gene="nuoG"
                     /locus_tag="LGJ20_000775"
     CDS             complement(32911..35637)
                     /gene="nuoG"
                     /locus_tag="LGJ20_000775"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBK2384893.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(35690..37027)
                     /gene="nuoF"
                     /locus_tag="LGJ20_000776"
     CDS             complement(35690..37027)
                     /gene="nuoF"
                     /locus_tag="LGJ20_000776"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBK2384894.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(37024..37524)
                     /gene="nuoE"
                     /locus_tag="LGJ20_000777"
     CDS             complement(37024..37524)
                     /gene="nuoE"
                     /locus_tag="LGJ20_000777"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBK2384895.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(37527..39329)
                     /gene="nuoC"
                     /locus_tag="LGJ20_000778"
     CDS             complement(37527..39329)
                     /gene="nuoC"
                     /locus_tag="LGJ20_000778"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBK2384896.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(39423..40085)
                     /gene="nuoB"
                     /locus_tag="LGJ20_000779"
     CDS             complement(39423..40085)
                     /gene="nuoB"
                     /locus_tag="LGJ20_000779"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBK2384897.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(40101..40544)
                     /gene="nuoA"
                     /locus_tag="LGJ20_000780"
     CDS             complement(40101..40544)
                     /gene="nuoA"
                     /locus_tag="LGJ20_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBK2384898.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(41175..42113)
                     /gene="lrhA"
                     /locus_tag="LGJ20_000781"
     CDS             complement(41175..42113)
                     /gene="lrhA"
                     /locus_tag="LGJ20_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBK2384899.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            43033..44250
                     /gene="alaA"
                     /locus_tag="LGJ20_000782"
     CDS             43033..44250
                     /gene="alaA"
                     /locus_tag="LGJ20_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBK2384900.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            44334..44933
                     /gene="yfbR"
                     /locus_tag="LGJ20_000783"
     CDS             44334..44933
                     /gene="yfbR"
                     /locus_tag="LGJ20_000783"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBK2384901.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLESRRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(44992..46824)
                     /locus_tag="LGJ20_000784"
     CDS             complement(44992..46824)
                     /locus_tag="LGJ20_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBK2384902.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(46911..47561)
                     /gene="hxpA"
                     /locus_tag="LGJ20_000785"
     CDS             complement(46911..47561)
                     /gene="hxpA"
                     /locus_tag="LGJ20_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBK2384903.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEYIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(47572..48066)
                     /locus_tag="LGJ20_000786"
     CDS             complement(47572..48066)
                     /locus_tag="LGJ20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBK2384904.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(48149..48604)
                     /locus_tag="LGJ20_000787"
     CDS             complement(48149..48604)
                     /locus_tag="LGJ20_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBK2384905.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            48942..50144
                     /gene="ackA"
                     /locus_tag="LGJ20_000788"
     CDS             48942..50144
                     /gene="ackA"
                     /locus_tag="LGJ20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK2384906.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            50219..52363
                     /gene="pta"
                     /locus_tag="LGJ20_000789"
     CDS             50219..52363
                     /gene="pta"
                     /locus_tag="LGJ20_000789"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK2384907.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            52553..54073
                     /gene="yfcC"
                     /locus_tag="LGJ20_000790"
     CDS             52553..54073
                     /gene="yfcC"
                     /locus_tag="LGJ20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK2384908.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGLFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(54106..54648)
                     /gene="yfcD"
                     /locus_tag="LGJ20_000791"
     CDS             complement(54106..54648)
                     /gene="yfcD"
                     /locus_tag="LGJ20_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBK2384909.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(54706..55257)
                     /gene="yfcE"
                     /locus_tag="LGJ20_000792"
     CDS             complement(54706..55257)
                     /gene="yfcE"
                     /locus_tag="LGJ20_000792"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBK2384910.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(55313..55957)
                     /gene="yfcF"
                     /locus_tag="LGJ20_000793"
     CDS             complement(55313..55957)
                     /gene="yfcF"
                     /locus_tag="LGJ20_000793"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBK2384911.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            56093..56740
                     /gene="yfcG"
                     /locus_tag="LGJ20_000794"
     CDS             56093..56740
                     /gene="yfcG"
                     /locus_tag="LGJ20_000794"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBK2384912.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            56797..57159
                     /gene="folX"
                     /locus_tag="LGJ20_000795"
     CDS             56797..57159
                     /gene="folX"
                     /locus_tag="LGJ20_000795"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBK2384913.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            57180..58073
                     /locus_tag="LGJ20_000796"
     CDS             57180..58073
                     /locus_tag="LGJ20_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBK2384914.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIQIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(58121..59023)
                     /gene="rpnB"
                     /locus_tag="LGJ20_000797"
     CDS             complement(58121..59023)
                     /gene="rpnB"
                     /locus_tag="LGJ20_000797"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBK2384915.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(59220..59993)
                     /gene="hisP"
                     /locus_tag="LGJ20_000798"
     CDS             complement(59220..59993)
                     /gene="hisP"
                     /locus_tag="LGJ20_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBK2384916.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(60001..60717)
                     /locus_tag="LGJ20_000799"
     CDS             complement(60001..60717)
                     /locus_tag="LGJ20_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2384917.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(60714..61400)
                     /gene="hisQ"
                     /locus_tag="LGJ20_000800"
     CDS             complement(60714..61400)
                     /gene="hisQ"
                     /locus_tag="LGJ20_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBK2384918.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(61490..62272)
                     /gene="hisJ"
                     /locus_tag="LGJ20_000801"
     CDS             complement(61490..62272)
                     /gene="hisJ"
                     /locus_tag="LGJ20_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBK2384919.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(62493..63275)
                     /gene="argT"
                     /locus_tag="LGJ20_000802"
     CDS             complement(62493..63275)
                     /gene="argT"
                     /locus_tag="LGJ20_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBK2384920.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(63541..64110)
                     /gene="ubiX"
                     /locus_tag="LGJ20_000803"
     CDS             complement(63541..64110)
                     /gene="ubiX"
                     /locus_tag="LGJ20_000803"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBK2384921.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(64205..65722)
                     /gene="purF"
                     /locus_tag="LGJ20_000804"
     CDS             complement(64205..65722)
                     /gene="purF"
                     /locus_tag="LGJ20_000804"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK2384922.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(65759..66247)
                     /gene="cvpA"
                     /locus_tag="LGJ20_000805"
     CDS             complement(65759..66247)
                     /gene="cvpA"
                     /locus_tag="LGJ20_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBK2384923.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(66506..67168)
                     /gene="dedD"
                     /locus_tag="LGJ20_000806"
     CDS             complement(66506..67168)
                     /gene="dedD"
                     /locus_tag="LGJ20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBK2384924.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(67158..68426)
                     /gene="folC"
                     /locus_tag="LGJ20_000807"
     CDS             complement(67158..68426)
                     /gene="folC"
                     /locus_tag="LGJ20_000807"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK2384925.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(68496..69410)
                     /gene="accD"
                     /locus_tag="LGJ20_000808"
     CDS             complement(68496..69410)
                     /gene="accD"
                     /locus_tag="LGJ20_000808"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBK2384926.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(69566..70225)
                     /locus_tag="LGJ20_000809"
     CDS             complement(69566..70225)
                     /locus_tag="LGJ20_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2384927.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(70308..71120)
                     /gene="truA"
                     /locus_tag="LGJ20_000810"
     CDS             complement(70308..71120)
                     /gene="truA"
                     /locus_tag="LGJ20_000810"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK2384928.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(71120..72133)
                     /locus_tag="LGJ20_000811"
     CDS             complement(71120..72133)
                     /locus_tag="LGJ20_000811"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK2384929.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(72199..73356)
                     /gene="pdxB"
                     /locus_tag="LGJ20_000812"
     CDS             complement(72199..73356)
                     /gene="pdxB"
                     /locus_tag="LGJ20_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBK2384930.1"
                     /translation="MSETEKGLKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAQLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(73515..74519)
                     /locus_tag="LGJ20_000813"
     CDS             complement(73515..74519)
                     /locus_tag="LGJ20_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK2384931.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFDKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWMYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSQKIADGLKPSSANRYMNRLSGMFTVLKKIGLWDAEHPVRGISILYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWSEACKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTRETGGLFEVKYREFCLALKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQKILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHAVSTGD
                     "
     gene            complement(74616..74936)
                     /locus_tag="LGJ20_000814"
     CDS             complement(74616..74936)
                     /locus_tag="LGJ20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2384932.1"
                     /translation="MASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEVRGK
                     QVTEGALLKVTKHPKFKKYAYWLSTDETMPEVGQISPALSLDGSCNSEGDRVSTETIQ
                     KLSR"
     gene            75050..75337
                     /locus_tag="LGJ20_000815"
     CDS             75050..75337
                     /locus_tag="LGJ20_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384933.1"
                     /translation="MSESELGGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNEGMRKAFFEQPKERRDAWLLWLGL"
     gene            75344..75550
                     /locus_tag="LGJ20_000816"
     CDS             75344..75550
                     /locus_tag="LGJ20_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384934.1"
                     /translation="MTSHIISAILTLVFIEIGVIAIYLFRKLTGHEERFIELSIEYIA
                     AYTKGLFPAAIGAMFIAFVIWFIG"
     gene            75803..76144
                     /locus_tag="LGJ20_000817"
     CDS             75803..76144
                     /locus_tag="LGJ20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HBK2384935.1"
                     /translation="MKQQRNSRFRNGAERHANRFATSASRSNIRYSLSDTHATPDGYP
                     VKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALSSGENQFGQDFTLYEALRTVDRLL
                     RGQSFIKQADL"
     gene            76155..76442
                     /locus_tag="LGJ20_000818"
     CDS             76155..76442
                     /locus_tag="LGJ20_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384936.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVAPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISKAEKARQANGNEPYIEIICMDSEAEIQKAD"
     gene            76454..76696
                     /locus_tag="LGJ20_000819"
     CDS             76454..76696
                     /locus_tag="LGJ20_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HBK2384937.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGSHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            76693..76806
                     /locus_tag="LGJ20_000820"
     CDS             76693..76806
                     /locus_tag="LGJ20_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384938.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTGE"
     gene            76899..77315
                     /locus_tag="LGJ20_000821"
     CDS             76899..77315
                     /locus_tag="LGJ20_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384939.1"
                     /translation="MNTKEEGIINTLKKISEAEDEMAKDAVKRSQHMAALHALTIAKI
                     TADAAKIIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMPQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            77339..77542
                     /locus_tag="LGJ20_000822"
     CDS             77339..77542
                     /locus_tag="LGJ20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384940.1"
                     /translation="MAIKHFSVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            77539..77805
                     /locus_tag="LGJ20_000823"
     CDS             77539..77805
                     /locus_tag="LGJ20_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK2384941.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            77802..78101
                     /locus_tag="LGJ20_000824"
     CDS             77802..78101
                     /locus_tag="LGJ20_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384942.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDKVFDFFLNQ"
     gene            78113..78730
                     /locus_tag="LGJ20_000825"
     CDS             78113..78730
                     /locus_tag="LGJ20_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBK2384943.1"
                     /translation="MHTQKNRLPCRNRSGYISAAPHKTGAGILNPIQSKAHNRASGFF
                     VRTVLPRLFRVRIMAGRTGPTSVGPDSLLSGVENPVRLATPRFSTLDGELFLSPSKEA
                     TPWQTANNSAHTLRVVTSRLKSTVDFSAHHASRKSCTSICCMSAATRYRTSIPPLFSA
                     IWRMICASCNNSSSSKTNSINSCSGPFLHLAAGGLRTSVVKRIAA"
     gene            78727..79116
                     /locus_tag="LGJ20_000826"
     CDS             78727..79116
                     /locus_tag="LGJ20_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HBK2384944.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQEWQRVTGYQP"
     gene            79113..81953
                     /locus_tag="LGJ20_000827"
     CDS             79113..81953
                     /locus_tag="LGJ20_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBK2384945.1"
                     /translation="MNEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     ALEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDHRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     EVALKAYQMLARMALHLHAMPPHYDALTTDKDRKNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPDEIETIVSHVCDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWVRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPQPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHEQRISETTERARRKA
                     DLLRGDLTSGNKKQTETGFTNPVNEQKTRREI"
     gene            82030..82989
                     /locus_tag="LGJ20_000828"
     CDS             82030..82989
                     /locus_tag="LGJ20_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBK2384946.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDSVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYNSLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            82994..83305
                     /locus_tag="LGJ20_000829"
     CDS             82994..83305
                     /locus_tag="LGJ20_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBK2384947.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKTNPAEAYRLAAWADDEMLPVTQISSKKFEAQSAPVAKITSQMAGNIRALFP
                     E"
     gene            complement(84427..>85083)
                     /locus_tag="LGJ20_000830"
     CDS             complement(84427..>85083)
                     /locus_tag="LGJ20_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2384948.1"
                     /translation="ALAKYMRRGSDLESWWYVQDGKDAFQFRPGKVCHLMNPDINQEI
                     YGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQVNRESMDSLKETLQG
                     ARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASRDDVLAAHRVPPQLM
                     GAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVIRFNPYALLDTQPTS
                     "
BASE COUNT        21377 a        22962 c        21000 g        19744 t
ORIGIN      
        1 ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg tgtacaactg
       61 ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac cgataaagca
      121 cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt aaaaggcatt
      181 ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata cccggaagtg
      241 ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg ctggctgtgg
      301 cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa agtggtttat
      361 cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga gctgttgcgt
      421 aaaatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat tgcgggtact
      481 tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggtgcgcc actgttccgc
      541 cagatagaag agagcggcgc ggatctggtc atcaccgact gcgaaacctg caaatggcag
      601 attgagatgt ccacgagcct tcgctgcgaa catccgatta cgctactggc ccaggcgctg
      661 gcttaaccga ccttttgatg cccggtaagc acattgctta tcgggcattt ttgcgagcgc
      721 gattccacgc accatgtatg cgttgcaacg ctttgaaaat tcctctgaaa atgtctcgca
      781 aaggctgaaa ctgacagatg tcaaaggccc gggatagacg taatgtcgcg tcatcataaa
      841 tatcaggtga cggacaacca tgaccgaatc aacaacatcc tccccgcatg atgcggtgtt
      901 taaaaccttt atgttcacac ccgaaaccgc aggggatttt ctcgaaatac atttaccaga
      961 accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta gttttattga
     1021 aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca gcgacggtga
     1081 cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata tggcttttcg
     1141 gctaatgcgc tatgccactg ccgccatgca gcgtcacctg gataaaggct atgacagagt
     1201 tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc cgtactcact
     1261 taactggctg gatgagtttg acgatccgca acttgcccgg cagttgtaca ccgaagcttt
     1321 tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc ggcgtatagc
     1381 tctgctggaa ctgattcaaa agcatattcg cgaccgcgat ttaatcggca tggtcgacag
     1441 gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa cactgtttaa
     1501 ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattgagg agattgccga
     1561 acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac ggcaggaagg
     1621 gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg ctttgcgcat
     1681 gctggaacag ggcattgatc gtgaccaggt gctcgcggcc acccagctaa gcgaagccga
     1741 tctggcagcg aataaccact aattaacaca ggcccacagc cgatccccat gggcctttga
     1801 tatcaataac taccttttat gcgtggccca tttttgcctg atttaaacat cgccagtcgt
     1861 tgatccaggg catcgctgta gagcatcgtg tcaacgccaa cagcgacaaa gttcgctccc
     1921 cacgccaggc attgctgcgc catatcagga gccacagcca gaaaaccagc cgctttaccc
     1981 gcagcacgga tccgccgaat gctggtttca ataattcgct gcacttccgg gtgcccggcg
     2041 ttatccgggt agcccaacga cgcagaaaga tccgctggtc caataaacac gccatcaatc
     2101 ccttcgacgt cgaggatctc gtccaggtta tccagtgccg ttttactttc cacctgcacc
     2161 aacagacaaa gtgaatcgtt aacttgcgcc atgtaattct caatgcgtcc ccagcgcgca
     2221 gcccgtgcca cactggcccc aacaccacgc tcaccgtagg gagggttgga caagttttgc
     2281 cggaggggga atgtgccggt gcagtaaacc ggcacagagt ggcggaatca gacagataag
     2341 gtcatggact caacaacctc aatccagcca tgctcactgg cgatgtcctg gccgtttaac
     2401 caacggcgca gcatattcag tgccatcatt gcgcacactt cctgacggat agccaggctg
     2461 tagcgtgtag tgctgaaacg cacacgcaga gcgaaagtgc cgtctggcgt ggctagcgca
     2521 aagttgagat gctcgttctc gaaacccgaa acagccagtg ccagcccggc aaaatggttg
     2581 gcccgccgtt ctgtaatcca gtgcgcagtt tgcgccaggg tttcttcctg tgaaggaacc
     2641 acttcacacg ccagcaatgg agcacctgcg cgagaaagtt gcaaagccaa taaaccgccg
     2701 gtgaattgct cgctcaacgt caggctgaac tggcggtttt gcaattcgcg actgatctgc
     2761 gcgggcagtc cttcggtgcc ttcgaaaatc acgctctgtc cggcaacacg tttaacgtcc
     2821 agccacagtt tttccatcgc ctgttgctcg cttgccggtc cggtgagttt cagttcgata
     2881 atcggcattg aagagcgata gcccattgtt acgcccggcg gcagttgtag agtgtccagg
     2941 ctttgtgcca gatcgctttc cgaacgacca aaggtagtta aacgcagaca aaccggcggc
     3001 tgtggtaaag aaaaacgctc gcgcagacgc ggcaggattt cgtgctcgac catcacctta
     3061 aattctgacg gtacgccggg agtaaagaac atcaggcaac gattaagctg cacggcaaaa
     3121 ccacaggcgg tgcctaccgg gttattgata aattcagcac tggcaggcag ctccgcttgt
     3181 ttacggttgc tcggtgccat tactcgtcca cgttcgtgga aatagcgttc catctctttg
     3241 agccaggctt catgcagcac catgccttca ccttttgctg tcgcagcggc gagtgcgctt
     3301 aaatcatcgc tggtcggtcc cagcccgccg ttaacgatca gcacatcggc gtgctgacta
     3361 cgttcgcgaa gaatggtgac taagtcatca aggttatccc ccaccgtatt gcggcgagat
     3421 aatggcagcc cctgatgaaa gaaaaaatcg gccagccagg cagcgttagt gtcaacgatt
     3481 tgcccgtgta acacttcatc cccggtggat agcatttcca cttttaacat ggttatctcc
     3541 tgcttttgtt atcgaatcac tataacgcaa agggagataa ggcgtagtaa gaaactgaat
     3601 ggcgcgacag aatgccgcgc cggaagggat cagaaactgg cgttaacccc aacatacggg
     3661 ccgtcagcca cggcgttgtc gcggttaccg tctttacccg acagattcag gtagcgataa
     3721 cccgcctcaa tactgactgg acgcataatg gtgtaacgcg cgccagcatt cgcttcctca
     3781 taactttgaa taccgctgga gagcgaatcc ggagagtagt aatactcgcc aaacaaacgg
     3841 aagctgttgc caattttcca ctgcaaacca cctcctaccg ctgcggcata accttcatcg
     3901 ccgtaattcg ggttggtgta aacgcctttt ccgccaacgg tcgccattaa cggcccgaga
     3961 ggcagattca accccagccc cacgcccgcg acgtcaccgt cgtcgtcgtt atgtgtccag
     4021 ttaccgctta aagccaggcc cgtcgattca gtcccaaaac cgacaccaat attggtgtat
     4081 tctttacccg cctggccgct gatgctcatt gcattgaccg atgcagaaac caacagcatt
     4141 ccggccagac ctgctagcgc aatttttttc attatcccct ttcccacaag caattaatca
     4201 aaattcccaa agctgcggga ttgtactgcc tggcacagag gattcaatga gcgaaaaaag
     4261 ggatctagtc acttattgta actaaatact tcttaaataa ttaaacgccg aaaaaataaa
     4321 cacactttac cgatacacac aaaaataata ataagtgcag ttatcattgc aaaagagatt
     4381 actgtcactt tcacatattt ctgagttacc gtattctcat ggtttctttt tatttataag
     4441 gaatacccct atgcgacaac ggactattgt atgccctttg attcaaaatg atggtgctta
     4501 tttgctgtgt aaaatggccg acgatcgcgg cgttttcccc ggtcaatggg cgctttcggg
     4561 tggcggcgtg gagtctggcg aacgaattga agaggcacta cgccgcgaaa ttcgcgaaga
     4621 actgggagaa cagctgcttt tgacagaaat cacgccgtgg accttcagcg atgatattcg
     4681 caccaagacg tatgcagatg gtcgcaagga agagatttat atgatttacc tgatttttga
     4741 ctgcgtttct gccaaccgag aagtgaaaat aaacgaagag tttcaggact acgcgtgggt
     4801 aaaacctgaa gatctggtgc attatgattt aaatgttgcc acccgaaaaa cgttacgttt
     4861 gaaaggtctt ctttaacaat gatgacagcc atcactggct gtcagattga aaatttagtg
     4921 gttaacgaat tccccatcca gataaacttt gcctttttcc acatgcatga ctaaaccatc
     4981 cagataatca ggtttaaatg tcgcatcacg cttattttta gcaatatatg tcaggcaatg
     5041 attatgggtg aaaataacga tatttttatc aggcgctttg ctttgtaagt ccttaattgc
     5101 actataaatc tcattaccgc actgaagaag tcgtttatct accgtcagct ttttacccgc
     5161 ggaaaaccag gtcgccgact gaatggtccg gacggtatta ctggaataaa gatcgaaatc
     5221 agggatgtca gcgctaaaag cgttgcccag ttcacgggca tcctgggtac ctttaaccgt
     5281 aatacctgtt ttatctgaca agcattgatt ggttgaacga tcgcaacgtt cagcatggcg
     5341 aaacaaaacg acaaccgggt gctgctgtgc cagtctggcc aatgttttat tgtcgatacg
     5401 tggcaaacca ttgctactcc aggcggcatg agtacccagt ccggcaattg cagcgagcgc
     5461 cagtaaaatg atgaaatatt tttttgactt caatgatgag cggcaaaaag ctaacacgta
     5521 aactccacct atagacaagc gcaaccagac aattaccgta aaattgagcg acatttctgg
     5581 cgataattag cagttggtgt aatattaaaa atcctacgat gtcggcaaaa tgcctcaaaa
     5641 ttttgccaaa tgcaaagcct aaataagaaa aaatataaaa atttcaatat ttacgtctaa
     5701 tattagtttc ttaaggttaa gttaatattc tatccttaaa atttcgctcc aaatggcaaa
     5761 atatacacaa cactctttat agcaaatata agtggacagg tattcaatgg cggaaggaaa
     5821 agcaatgtca gaatttttgc ctttttcgcg accagcaatg ggcgtggagg aactcgctgc
     5881 agttaaagag gttctcgaat ccggttggct cacaaccggt ccgaagaatc aggcgcttga
     5941 gcaagccttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt cagccaccgc
     6001 cggaatgcat atcacgctaa tggcgttgga aattggcaag ggcgatgaag tgattacgcc
     6061 ttccctgacc tgggtttcaa ccctcaatat gatttccttg ttgggtgcaa cgccggtaat
     6121 ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa gcgatcgagt cagccataac
     6181 gccacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag atattgacgc
     6241 cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg cccatgccgt
     6301 cggtacgtat tacaaagggc gacatattgg cgcaaaaggt accgctattt tttcatttca
     6361 tgccattaaa aatattacct gtgcggaagg tggcctgatt gtaactgata atgaaaacct
     6421 tgcccgccag ctacggatgc tgaaatttca cggtctgggt gtcgatgcct atgacagaca
     6481 aacctggggc cgtgcaccgc aggctgaagt cttaacaccg ggctataagt acaatctgac
     6541 cgatattaac gccgcaattg ccctgacaca gttagcaaaa ttagagcacc tcaacacccg
     6601 tcggcgcgaa attgcccagc aatatcagca agcactggca gctctcccct ttcagccatt
     6661 aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc gtgtcgatga
     6721 acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaac gaggcattgg
     6781 taccgggtta catttccgcg ccgctcacac acaaaaatat tatcgcgagc gttttcccac
     6841 gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttctttgc cgctgttccc
     6901 ggatatgact accgccgatg ccgaccgtgt catcacagcc cttcagcaac tcgcaggaca
     6961 ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc ccgtttataa
     7021 cgagcaggaa agcttaccgg aattaatcag gcgcaccacc acagcctgtg aatcgttggg
     7081 gaaagagtat gagatcctgc tgattgatga cggcagtagc gataattccg cgcatatgct
     7141 ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct attttgctta accgcaatta
     7201 cgggcaacat tcagcgatta tggcgggatt cagtcacgtt accggcgact taattattac
     7261 ccttgatgcc gatctccaga atccgccaga agaaatcccc cgcctggtgg caaaagccga
     7321 tgaaggttac gacgtggtag ggactgtacg ccagaaccgc caggacagct ggtttcgtaa
     7381 aacagcttcg aagatgatta accggcttat tcagcgcacc accggcaaag cgatgggtga
     7441 ctacggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt tgcactgcca
     7501 tgaacgtagc acctttatcc cgattctggc gaatatcttc gcccgccgtg ccattgaaat
     7561 tccagtacat cattccgagc gtgagtttgg tgaatccaaa tacagtttta tgcgcctgat
     7621 taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta tgctgagtct
     7681 gctcggcagc attattgcga ttggaggttt tagcattgcg gtgctgctgg tgattttacg
     7741 cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg ccgtgctgtt
     7801 tacttttatt ggcgctcagt ttatcggcat gggattactc ggtgaatata tcggcaggat
     7861 ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc gtccatccag
     7921 caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg atgcctcggt
     7981 attgaagctc tgctggctgc cggttacgaa attagcgcca tttttaccca taccgataat
     8041 cccggtgaaa aagcctttta tggttcggtg gctcgtctgg cagcggaaag aggcattccg
     8101 gtttatgcgc cggatgacgt taatcatccg ctgtgggtgg aacgcattgc ccaactgtcg
     8161 ccagatgtga ttttctcttt ttattatcgc catcttattt gcgacgaaat tttgcagctc
     8221 gctcccgcag gtgcatttaa tctgcatggt tcgctgttac caaaatatcg tggtcgcgcg
     8281 ccgctgaact gggtgctggt caacggtgaa acggaaactg gcgttacatt gcaccgaatg
     8341 gtgaaacgtg ccgatgccgg ggccattgtg gcgcaactgc gcattgccat tgcgccagac
     8401 gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct actggaacag
     8461 acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc
     8521 acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa accggcatcc
     8581 gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt
     8641 ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag
     8701 ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg cgcgctggaa
     8761 atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt agcgcagacg
     8821 ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc ccgacgccgt
     8881 acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac agaacgcctg
     8941 ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc gataagccgt
     9001 tttctgaatc atccgcattt tcactttgtt gaaggcgata tcagtattca ttccgaatgg
     9061 attgagtatc atgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat agccacgccg
     9121 attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga gaatctgcgc
     9181 attatccgct actgcgtgaa gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt
     9241 tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat cgtcggcccg
     9301 gtgaataaac cacgctggat ttattcggta tcaaaacaat tacttgatcg ggtgatctgg
     9361 gcctatggcg aaaaagaggg tttacagttc accctcttcc gcccgtttaa ctggatggga
     9421 ccgcgactgg ataaccttaa tgcggcacga atcggcagct cccgcgctat tacgcaactc
     9481 attctcaatc tggtagaagg ttcaccgatt aagctgattg atggcggaaa acaaaaacgc
     9541 tgctttactg atattcgcga tggtatcgag gcgttatacc gcattatcga aaatgcggga
     9601 aatcgctgcg acggtgaaat tatcaacatt ggcaatcctg agaacgaagc gagcattgag
     9661 gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca tcatttccca
     9721 ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact acggcaaagg atatcaggac
     9781 gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg ggagccgaaa
     9841 attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac cgttgatctt
     9901 acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatacc tttcgtggca
     9961 cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt caggccagca
    10021 tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg gtgaagccac
    10081 agtttttgtg gaagatgctg cgctcaaatg cggcatcgct ttatggctgg gatattttac
    10141 tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat atcattcgtg
    10201 aagcggctaa acatcacgaa gtcggcctgc acgcctggga tcaccatgcc tggcaagccc
    10261 gtagcagtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt cttcgcactc
    10321 tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc
    10381 agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg
    10441 ccatgccgtt ccgtccattg ctcgaatcag gaaaccctgg cactgcgcaa attccggtga
    10501 ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat tttaacggtt
    10561 ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt catgcagaag
    10621 ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca gctcaggaag
    10681 gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc ggacaagttg
    10741 ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc
    10801 gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg tttattgcct gctattacct
    10861 gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg cggaaatcag
    10921 tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc tacgttattt
    10981 cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat ttggcgcgaa
    11041 taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg cgctggtgac
    11101 ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca cagtaattta
    11161 tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc
    11221 attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa
    11281 aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa
    11341 aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa
    11401 acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt
    11461 tctcccttgg ggactggcga tagcgcagcg ggagcctgac ttctggcact attttttctg
    11521 ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagctc cgttctggta
    11581 ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact
    11641 gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga gctggacgat
    11701 aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg cccacctata ttctttcctg
    11761 ctttgcatct ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa aaaataatcc
    11821 tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc
    11881 cacatttgtg gtctccccgt ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag
    11941 ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg gctggtacac
    12001 cttaacaaac gtcgaaaaga cctggccttt tgccgcgctt tgcccgctgg ggctggcgtt
    12061 gctggtagga ttttcaattc ctgacagagt tatggaagga aaacatccgc aattttttgt
    12121 cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata gcgtcggtgt
    12181 tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg
    12241 tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca gcggtgatga
    12301 gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attactctcg tgctttcggt
    12361 tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca tcgatcgtca
    12421 ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt agtctttgcc
    12481 agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgtggcgata
    12541 aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt
    12601 gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg
    12661 ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca cgaaccggta
    12721 tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat cctcgggagt
    12781 acggtgtaat gggcctgata tggggattat tcagcgtcat tattgcctca gttgcgcaat
    12841 taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg gattttatcg
    12901 cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc
    12961 tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc aaagcctatg
    13021 ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg
    13081 aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc
    13141 tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct gtacactgcc
    13201 gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta tgcagctttt
    13261 cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc actttcacgg
    13321 cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa
    13381 gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat tccattgcat
    13441 tatcctgttt gctaagagtt ttcacatcaa tttacagatt aacagataaa ctctttatca
    13501 acggcaatgg aatggcagaa atgagaagtg aaacggaggg ttaattattg ctgacgttgc
    13561 acccactctt ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag ctccggcggt
    13621 aaagtcagcc agcgcaccgg ttgttgaaaa cgagccagct tatctttcac ccattcgcca
    13681 agatcaacgg tttgctggtc atactccacc accgctaccg gccgatgacc aaactccttg
    13741 tcggcaacgg gaacgataaa cacctgcaaa accgcaggat gtgcagcaat aacacgctcg
    13801 acttcttccg gctgaatacc ctcaccgcca ctgaagaaca gattgtctaa acgtccgaca
    13861 atggtcagct tgccattatg catctcaccg cgatcgcgcg tagcgtacca gccttcgtca
    13921 ttaaccagtg aaaccagttg cccgttacgc cagtaacctt ctgccatact ggcagcccgc
    13981 agccacactt cattattaac gattttcact tcccgacccg gcagcggcga accaacgtct
    14041 gccaggccgt cggcttcttt cgcacacacc gtggaggcaa actcggtcag accatagccg
    14101 caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg
    14161 ccgccaagta acaccgcttt cagggaaacg gaactgcggt taaccagcaa acgccagagt
    14221 tgtgttggca ccagtgaagc gtgagtacag cctgccagca tttgctccaa tggctgttta
    14281 tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc ctgaccggag
    14341 acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg aatcaacgat
    14401 aacacccctt cggcactggc aagatgggcc tgataagtat ggacagcggc tttcggcaac
    14461 ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg tcggctgcca cgtagcggca
    14521 tgtgcgcctt caacccgctg aatgtgcagc gacgttaacg ccggaaacgt gttttcccca
    14581 tccggcacca gcgcaaattg cagcgtcaga ttgggcagca attcttcaag caacggttgc
    14641 ggcagctgag ggttcacggg caacacccgc gccccgcatt gcagtaacgc cagccaggcg
    14701 agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgccctc aacaacgccc
    14761 tgcgccgcaa agcctgaggc taattcatcg acgcgagcac aaagctcgcg ccagttaagt
    14821 tgctcgtcat taagacgtaa ggcgatggct tctccccgca cttgccgcca gtgacgccac
    14881 ggccagtcag agaagatcat aacaaccgct ccagtgcatc aacttccacg acaggcagcg
    14941 tgctacccgg ccagcgacgt acctgctgcg cctgcatcag atccagcgtg tccagccctg
    15001 gaatggtgtc cggcgttaac caggcggcaa tccgcgccag ttgcgttaag cctaagctcg
    15061 attcaatgga agaactgatc accgccgtca gccccagcgc gtgcgccgcc tgtacctgct
    15121 cgcgtacttt ctccagactg cccgtgagcg tgggtttgat aactaccgcg cgcacgccct
    15181 cttcagccac aaaggcaaaa tccggctcgc gcaggctttc atcccaggca atggcaatgc
    15241 cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg
    15301 cgatgcggtg gcgataatcc gggttaacat atttggcaaa ctgctggcct ttcagcggtg
    15361 tccaggcgcg atttgcgtca agacgcaaat gcagatccgg aattgcctcc agcaacagat
    15421 taaccaccat gccgtcgcgc acggcttcgt acaatcccac tttgaccttc gccactttct
    15481 cgcctggcat atcagcaagt ttgaggatca gatcgtcagg atcgccatta cacagtggtg
    15541 ccgcacggta attggccgcc tgcgggagcg tatctgccag ctccgccaat gcacagctta
    15601 cgccaaaggc aacggaaggc atctgcggta gctcgcaatc gcctgccagc cagttattta
    15661 cccaggcaag cagcacgctt tgcgcctctt cccaggtttc ctggctgaag cccggcagtg
    15721 gggagatctc cccccaccct tcgcgctcgc cctcacgcag gcaaacgtac agcccgtcgc
    15781 gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc catggggatc tgccagcggt
    15841 atacctgcgc gctacgcatt acggattccg tttaaatttg ctgaaatcag gctgacgttt
    15901 ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca gcatggtggc
    15961 gttgcccgcc agctcctgca gccccgcctg cccgtcacag tcggcgttca gcgcagcttt
    16021 caggcagcgc agcgccatcg ggctgttttg cagcatttcg cggcaccaac ggacggtttc
    16081 tttttccaga tccgccagcg gtaccacggt gttgacaagg cccatatcca gcgcctgttt
    16141 tgcgtcgtac tgacggcaca ggaaccagat ttctcgcgct tttttctgcc cgacgatacg
    16201 agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc cagtctgacc
    16261 gaagatggca ttatctgctg cgatagtcag gtcgcacatc atgtgcagaa cgtgaccacc
    16321 gccgatggag tagccagcca ccatcgcgac aaccggtttc ggacaggtac ggatctgacg
    16381 ctggaagtcc agcacgttca agtgatgtac gccggaatca tctttatagc cgccgtaatc
    16441 accacgcact ttctggtcac caccggagca gaacgcttta tcgcctgcgc cagtcagaat
    16501 gatcacgccg atattgtcgt cataacgcgc atctgccagt gcctggatca tctcttttac
    16561 cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt ttgcgatacc
    16621 gtcggtggat ttttcataac gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac
    16681 cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag tcagaaacgc
    16741 aagatctgcg ccagacttgc gataacgcca gcgggatttt cccgatgcgc gttatgtccg
    16801 gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccagggcgcg gaatttgctg
    16861 tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa
    16921 tcaggctgga cggcgagaga agtcgcctcc agcatggcgg caagggttgc gccattattg
    16981 ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc
    17041 tgttgatacc agtcggcaaa taccgccgtt aacggttctg tgcgaaaacg ctgcgcccat
    17101 tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat tttgcagccc cggatgcccg
    17161 ccttcgacaa taaccccaca aagccccgcc agcccctggc aagccgccat catcgccacc
    17221 cgtccgccaa gcgagtaccc caccagccaa aagtcaagga tgttgtaact aaccaaggtt
    17281 ttacgcagta agtcggtgac atcatcaaat ccatcgacgc taatcgccgc cgaaccacca
    17341 tgacctggga gatcaacata caaccgtgag tagtcggcaa acgcctcgcc cacttcttgc
    17401 cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa acctggtttt
    17461 ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccaga agttgctgga
    17521 gcgtttgcgc accatcggtg tcgttaacca ccatttcaat caccgtggtg gttggcgtgc
    17581 gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt ttgcggacga tgatatttca
    17641 gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga tagaaacgct
    17701 cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg cccgccgttg ttgttcacca
    17761 caattaatac cagcggcgca gaaacctgac gcaataacgc cagcgcgttg agatcgtaaa
    17821 gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc tgaacgcctg
    17881 cggcggtcga aagcagtccg tcgataccgc tggcccctcg gttgctgtac accgggtaac
    17941 ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac caggctgtta ccaacaaaca
    18001 attgcccctg ttcaggcaga tagtcgcaga tgcgatgcgc cagttgcgct tcgccaaacg
    18061 catcacggcg ggcaataacc gcctgcattg cctgttccgc caggcgcggg atttcaacgc
    18121 accagggctg gcgtttttct gccggatgca gctccagcca gtcggcaata ttggcaatta
    18181 agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc aatgtcatca acaatccagt
    18241 actcttctgg ttcacagctt gcctgccatt gcaggagccg tttgcccgtc aggctgcttc
    18301 ccagttgcac cacaatttgc gcctgctgca gctcgctggt cgctttggca ttgcctaacc
    18361 aaagatcggc acacggcagc ggctgcccgg tttgtgacag cacatcgcca atcagcggcc
    18421 agccaagagt ttgcgcccac agggcaactt ttttgccctc ttccgcactc atgcgcccgg
    18481 caaccaccac gccgcgcttt tgtcgccaga agaaccagtc gcgctgtttt tcactttcca
    18541 gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca ccagtcaccc agacgctgtt
    18601 gccagctaag cccggtatcg tccatttcgc catacagcgg ttcagcaaac gggcagttga
    18661 tatggactcc ccccgcatga agcgtaccga gagcgtggtc gatggtagaa accagccaac
    18721 gtgcggggat atcccgggtc gggcgcggca atgaaatact gtgcgtgggg tgagaggcga
    18781 acattcccgg ctggcgaatt gcctgattcg cgccgcagtc aattagctcc ggcgggcgat
    18841 cggcggttaa caggatcagt ttttctccgg ttaacccagc ttcaatcagt gccggataga
    18901 gatttgctac cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg ctgactttcg
    18961 ccagccccag cgccagatgt cccagtccac gctcatcgaa atgggtgtgg tgaatgaatg
    19021 tggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagccaggg gcgatacaga
    19081 tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag cgtcggttaa
    19141 atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat aaggagctac
    19201 aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc catctgtaat
    19261 aaagtacgca gccctgccgc tttgttgtcg atttcctgcc actcttgctc ggggtcggaa
    19321 ccacggacaa tgcccgcgcc agcatataat cgcacgacat tgccgctaat ttttgctgag
    19381 cgcagggaaa cgcagaattc gctttgttgt aatgagagat agcccgcaga accggcgtac
    19441 cattctcggg tgaacggttc gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag
    19501 ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc cgctttgttg
    19561 agtgaagtcc agatacagcg gcgaagatgc tgcactttac gcagacgcag tacctgcggc
    19621 ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt ggcagatatc ttccaccacc
    19681 agcatgttct cgcgctggtt tttatcatcc gccatcagcc actctcctaa ctgctgcgcc
    19741 tgcttatcat caggattatt tgctactgtt cccgccagcg cttcagtacg cagcgctttg
    19801 tcacgccgcc gccataaccg ttccggtgaa gagccaagaa aagcattttc gccatcaaag
    19861 gccatgtaaa aatggtagca attcagattc agtcgacgac tggcagccat catcgccgcc
    19921 gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag cgagcaccac tttgtcgagc
    19981 tcaccttcgg cgatggtttt cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc
    20041 ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag gcaagggctt gatactcacc
    20101 agtgtggcga taaattcttt tgcctgaatc gcatcgtgct gaagggagct ttcgctgaat
    20161 agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg gggtaaaagt
    20221 aaattgccct gcgacgggtc aaaagcattc agcccccaaa tgcgtaagtc ggcgtgttcc
    20281 gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc
    20341 aggacggcag cttcttcatc accattacgt tgttgccagt agaattgcgg gtacgtttgc
    20401 tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg aatatcgata
    20461 acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag attttccagc
    20521 gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat tatacggggt
    20581 actacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc cagcaacagt
    20641 cctagtacca gcccaacggc cgcgcccaca ccaattcctt gccagggttt ttcgtggacg
    20701 tagtcatcag cacgataaac tgcctgcttc gcccgatagt aataactgtc tgaagcctgg
    20761 ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag ctcaacatat
    20821 ttctgatcgg cgggatcgcc agaggagcgg agcacctctt ccagtgtttc actaagcagc
    20881 gtcaggtcgt catcgatacg tgtatcacca aactgattag acattctcgt tctccatttg
    20941 cgtaaaacct gtcactaact atagacaagg gttgttgatt acgcctgaat tacctcgcgt
    21001 gccatcccaa tgtgtgggat accatcttct tcatagacct ccgtcacagg gataaaaccg
    21061 aaactctggt agaagttttg cagatgcgcc tgcgccccga ggtaaacagg cttatcaggc
    21121 cagtgatgcg tacatgtttc gagtgtttta ctcatcagct gctgccccac tttttcgcca
    21181 cgcaacgctt cactgacaat cacccgacct ataacgaccg gctcaagatc atcatcactt
    21241 ttcagaatcc tcgcatacgc caccagttca tcatttttcc aaccgagaat atggcgatta
    21301 tcccccgtca aatcatcacc atcgatatcc tgataagggc aattctgctc gacaacaaac
    21361 accgcgcaac gcagttgtaa taaggcataa agctgggaaa ccgataattc agagtgatgc
    21421 agatcttgcc attcaatcat aatgctctcc ttttagatgt atgccttata ctagccggaa
    21481 tattttttga aacgtgatga actcatggaa ttaatttttt taggtacttc agccggtgtg
    21541 ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc tgcaacatcc gacccagagc
    21601 ggactttggt tgtttgactg cggtgaaggc acccagcatc agctactgca taccgccttt
    21661 aaccctggaa aactggacaa gatttttatc agtcaccttc atggcgatca tctttttggt
    21721 ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt aacgatttat
    21781 ggtccgcaag gtatccgtga atttgtggaa accgcgctgc ggattagcgg ctcatggacc
    21841 gattatccgc tggaaattgt cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc
    21901 aaagtaaccg cttatccgct ggaacaccca ctggaatgtt atggctatcg tattgaagaa
    21961 catgataaac cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt gccgcctggc
    22021 ccactgtttc aggaattaaa agcgggcaaa acaatcacgc tggaagatgg aaggcagatt
    22081 aacggcgcag attacttagc tgctccagtg ccaggtaaag cgctcgctat tttcggcgat
    22141 accggcccct gcgatgccgc acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa
    22201 gcgacgctgg atataaccat ggaagccaaa gccaatagtc gcggccatag ctctacacgc
    22261 caggctgcga cactagcccg tgaggctgga gtcggtaagc taatcattac ccacgtcagc
    22321 tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg aatgcaggtc aattttcccg
    22381 gcgactgaac tagcgaatga tttcaccgtg tttaacgttt aactctgctt attattaaaa
    22441 acagggcgaa acttgccctg ttatcgcaac ccgcgctaca tatatccacc cacacgggca
    22501 tattgagcga tttttgatat ttattcatct cctcacgcac tatattcaga gcaataagtt
    22561 gcctctattt atcttgattc agcacaacct ttgcaactaa aaggagccca tgatggttac
    22621 agttgtcagc aattattgtc aattatctca aacgcaactc agtcagacat ttgcagaaaa
    22681 atttactgtg accgaggaat tactgcagtc tttaaaaaaa acagcgttat ccggagatga
    22741 agaaagcatt gagttactgc ataatattgc gttaggctat gatgaatttg gaaaaaaagc
    22801 tgaagatatt ctttaccata ttgttagaaa cccaacaaat gagaccctat cgattatccg
    22861 acttatcaaa aatgcttgtt taaaattata taatttggca catgccgcaa ccaactcccc
    22921 ccttaaacca gctggtccgg ataattcaga ggatctcctg ttcaaaaagc tattctcccc
    22981 ttcgaaatta atgacaatta tcggtgatga aattcctctt atatctgaaa aacagtcgct
    23041 ttcgaaggtg cttttaaatg atgagaataa tgaactgagt gatggtacaa acttctggga
    23101 taaaaatcgt caattaacca cagatgaaat agattgctat cttcagaaga tcgccgctaa
    23161 tgcaaaaaat actgaagtca attatcctac tggtctctac gtcccctact ccaccagaac
    23221 tcacctggaa gacgctctca atgaaaatat taagagcgat ccatcatggc cgaaagcagt
    23281 ccagttattc cccataaata ctggcggaca ctggatatta gtttcgctac agaaaatagt
    23341 aaatgaaaaa aataatacac aacaaataaa atgcgtcata ttcaactcat tgcgcgcact
    23401 aggccatgat aaagaaaatt cacttaagcg tgtcattaat agttttaatt ctgaattcat
    23461 gggagaaatg tcgaataata atataaaagt tcatttaact gaacccgaga taatattttt
    23521 acatgccgat cttcagcaat atttaagcca aagttgcggt gcatttgtgt gcatggcagc
    23581 ccaggaagtg attgaacaaa gggaaagcaa ttctgacagc gccccctata cgttattaaa
    23641 aaactatgct gacagattta aaaaatattc agcagaagag cagtacgaaa ttgattttca
    23701 acatagactg gtaaacagaa attgttattt agataaatat ggcgatgcac gaatcaatgc
    23761 ttcttacaca caattagaaa taaaacactc acaaccccaa aatagagcat ccggcaaaag
    23821 agtgagttaa catcattgtt aattaattcg accagtcagc aaatatgctg actggtcgaa
    23881 catagtttgc taaacagtca tcactgactg atgtcagtca caccatccgc cagttcaatc
    23941 ttcgggtatt tccagcgtat ctcggcacgc gattgctggt ctgttgatgt agtggattgt
    24001 tgttgctgac ttttcttacc ctcagagttc tgccccctgt caactcaatc tgccaataaa
    24061 taacataatt atatttttcc ttatatgaat tcctttttaa aatagcgaag ccactgtaaa
    24121 agatactttt tgtatagatc taacaaccgt aataaaacaa acactcataa ctcattataa
    24181 ataatgataa aataacgtct tttattgata atttatatat gaaaaaaatc cttttgttat
    24241 attatttctg atttcactcc ctctgattat ttttaccaac cctgggtcac tgcattatcg
    24301 ccaacgcccc gacacgtcag tcctgagcaa ttagaaaaaa cagttcgtta tcttacacaa
    24361 actgtgcatc cacgtaatgc cgacaatatt gataatctga ataggtccgt agagtatata
    24421 aaagagttct ttgtcagtag cggtgccaga gttaccgcgc agggcgtccc cgattggcta
    24481 ttacgactct gcgcctggta gccaggatta cccttacccg caatgtccta gctttatccc
    24541 gatcgggggg actttattgc ctggtcggca gaatacagga tatcaacgcc gttcgtcagg
    24601 taaaaacggc attgttatca tcccgggatt tatctgttta ttctatgaat gccccatggt
    24661 ttattcccgg tattgatttc tctgaccacc tgaattattg gcaacacgat attcccgccg
    24721 taatgattac tgacaccgct ttttatcgta ataaacaata ccacttgccc ggtgatacag
    24781 cagacagatt gaattatcag aaaatggctc agatggcgtt ataaccttgt tacacaacag
    24841 taaataataa aaatttctcg aggcgggtcc agaaacagcg acatgtacat tttattttcc
    24901 ctcgtctgtt ttaagatgcg aaacataaaa ttcaatggag gaaaaataaa tgggaatgat
    24961 tggttatttt gcagaaattg actcagaaaa gattaaccaa ctgcttgagt ctactgaaaa
    25021 acctttgatg gataatatac atgacacgct ttcaggatta cgccgactgg atattgataa
    25081 acgctgggat tttttacact ttgggctgac aggcacctct gcttttgatc ccgcgaagaa
    25141 tgatccgctg agtcgtgccg tgcttggtga acacagcctc gaagatggca tagatggctt
    25201 cctgggattg acctggaatc aggagttagc cgcgacaatc gaccgactgg aatcactgga
    25261 tcgaagtgag ctgcgtaaac agttttccat taagcgactc aatgaaatgg agatttatcc
    25321 gggagtgacc ttttcagaag agttagaggg gcaactcttt gcttcgatca tgcttgatat
    25381 ggaaaagctg attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt
    25441 tattgttggg tgatggtgtc agggcaccaa aaaaaaccgc cgaacatgtc ggcggctttc
    25501 tgacttacaa actgatagct tacatcagcg gcattgctaa ccgcacaatg ctaatcagcg
    25561 gttgtggcca tacacccagc accagtacca acagtgcaga gatcagcacc acgataccgc
    25621 ctgcgctgta ctgccagttt gacggtgcat cgcgacccgg ttgttccggg gcgtgcaggt
    25681 acaggctcac cgccacgcgc aggtagtagt aaaggccgat tgccgaaccg acaaccacgg
    25741 cgcccaccag ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccaa
    25801 taaagcccag cgtcatcgga ataccggcca gagacagcat catcaccgtc atcaccgccg
    25861 cgaggatcgg gcgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc
    25921 cacggtacgg gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat
    25981 aaccggccag gtaaactcct accgcttcca tcgacatctc gcccgtttgc agcgcaatca
    26041 gcgccaccag cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatat
    26101 tggtctggct cagcgccatc aggttaccga agatgataga ggcaaaggcg ataatcgcca
    26161 gcactacccg aatcgcttcg ctgtcaccca ccggtgcgta gaggaacaga cgcatcacca
    26221 caccgaagat agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc
    26281 cctggtatac gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc
    26341 caacaatcat cagtccgaaa cctgccagca acagcggctc gttgagcata ccgtcgccga
    26401 ggtttttacc cagcgcgaca aacgacaggt cgccagactg tgcatacacc agcgccatac
    26461 caaacagcag gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca
    26521 gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt
    26581 caataccgag gaacaaagac gccagatggt tggcattcgc cagcaggatc ccgcccagcg
    26641 cggcaattaa caccaacagg tagaactcat ccttgttgtc gttatagcct tcaagccacg
    26701 ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg
    26761 cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccgacaaacc
    26821 agagcgaaac cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat
    26881 tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt caagccgacg atcagcaacg
    26941 gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag
    27001 taacggaatt aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa
    27061 tcggctgcgg atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca
    27121 gctcacgcag cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga
    27181 agtaagcgcg atgcaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag
    27241 agataacggt aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga
    27301 cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacagtgctg
    27361 gcagccattt catcttgctc cacagaccgc ccatcatgcg catatcgcgg gtatggatac
    27421 gttcataaag ctgaccacaa agaataaaca gacccgccgc cgacaagccg tgcgcaatca
    27481 tctggattac cgcgccctgg taggccaact ggctgccggt gtagatagca atcagcacga
    27541 agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaaag
    27601 ccatccacgc accgtagaag atgccgataa cccccagcca catagcgatt ggcgcaaact
    27661 ctgccgatgc gttcgggaac agcggcaggg agaaacgcag caaaccgtaa gcggcagttt
    27721 tcagcaagat ccccgcgagg tcaacggaac cggcggtcgg agcctgagag tgcgcatccg
    27781 gtagccagcc atgcagcgga accaccggca ttttgactgc gaaggcgatg aagaagccca
    27841 gcattaacag gtattccaca ccactggaca ttggcgtatt cagcagctct tcatagttga
    27901 aggtccagac gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcaatcaaca
    27961 tcaccaggcc actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt
    28021 taccgtcaga ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca
    28081 tttcccagaa gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc
    28141 ccaggatcca catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg
    28201 aacatagtac cgccagcaca ccgagcagac cggtcagcac gaccatcagc agcgacagcc
    28261 cgtcaatggc gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag
    28321 actgccactg cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt
    28381 gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt ggcaccttga
    28441 cgccaaagcg ttcggtctgc cagcacagga agccgccaat aaagggaatt aatattagcc
    28501 agggtagtaa catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa
    28561 cgaattctca cgacagaaaa ttctcttcgg actgggggta gtgtcggatg caccgcgaag
    28621 gcggcttatc cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa
    28681 cgcagtacca tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac
    28741 cagcgcagat agccgttctc acttaacagc agacctttac ctgcaaagcg ggaaagaacc
    28801 gccgggatgt tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccagg
    28861 aacggcttga cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag
    28921 gtactcagca ggcggcccgg cgcactgttg gcgatggagg tcaccagagt acgtttaccc
    28981 agccacagcc aggctgccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc
    29041 agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgcc ctgcagcggc
    29101 ggtacaatca gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg
    29161 tgagttaccc ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa
    29221 atcatacgga aggtgtagag cgaggtcata aacgcaccga ccagacctgc caccatcaga
    29281 ttgatatgac cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca
    29341 gtgaccagcg gcagtgccga cagtgctgcg ccgcccacca ggaagcagag ataaaccagc
    29401 ggaatagatt tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga
    29461 atgacggaac cggatgccag gaacagcagc gctttaaaga acgcgtgagt catcaagtgg
    29521 aaaatcgccg catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc
    29581 atggtagagt aagcgagaac acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc
    29641 agcagcagcg taaccgcccc gacaataccc accagatgca gaacttccgg cgtcatcagg
    29701 aacaggccgt gggtacgggc gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg
    29761 atcagcgcgg agacaggcgt cgggccagcc atcgcatcgg caagccatgt ctgcaacggc
    29821 aactgcgcag atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg
    29881 ttattgccgt cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg
    29941 cccagttcgt tgtaaagaat gaacagtgcg aaagcgagga acacgtcacc cacacgggtc
    30001 acgacgaacg ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaaaccgatc
    30061 agcagatagg agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttgtcg
    30121 gcaagcacca gaactaccat gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag
    30181 tagccctctt caccgcgcat gtaccaggag gcgtacatgt gaataaggaa acccacacca
    30241 gtgaccaccg agagcatggt cagcgacagg ccgtccagca ccagattgaa accgatgtta
    30301 aagtcgccta ccgacatcca cgtccacagc ggctggctgt atgactgctc gccgttagcg
    30361 aagaaatcaa cgccgataaa ggcggttacc agcgccgcca ggcccacaga gccaacgccg
    30421 acgatcgccg aaacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca
    30481 atcaatggca aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat
    30541 cgatgttcag gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg
    30601 cttctgccgc cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc
    30661 agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa
    30721 tcagcataaa cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga
    30781 ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt
    30841 cgcgctgtcg tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccgacgtg
    30901 gaaggccaca accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta
    30961 aggcccgaac agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg
    31021 atcattaaca ccaaggatgg cgtaaacaat caccaccagc atgatggccg acaaaattgc
    31081 cggaccaatc cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc
    31141 caggttgagc atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat
    31201 aatttccagc gcaccggcga agtaagcgcc cagtgagaag aacaccccgg aaatcgccag
    31261 cagcgaaata atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa
    31321 ggtcgcaagt atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct
    31381 tacggtaaca ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct
    31441 ttgccgtcga ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga
    31501 ccggagatca gcagatcctc tttttcgtaa accagatcct ggcgcttgta ttcccccatt
    31561 tcgaaatccg gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag
    31621 aaaatgcagc gtgagaagtt gatgcggaaa aattccgggt accagcgacc gtctttggtt
    31681 tctgcttttt gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct
    31741 acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc
    31801 ggcagataga ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg
    31861 ccgatcatcc agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc
    31921 atggtcttat tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt
    31981 tgatcagcgt cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg
    32041 gacgcggtaa cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcagcg
    32101 cgaaccagat gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca
    32161 tcaatgcaga gatggtcaca atcccgatgt actcacccac gaagaacaga ccgaacttca
    32221 taccggaata ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa
    32281 acgggtgacg gtgacatacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt
    32341 gcgggataac gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac
    32401 cggcctgcgc caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct
    32461 gcgcagaagc acgcatcgca cccagcaacg agtatttgtt gttacttgac cagcccgcaa
    32521 acagcaccgc gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca
    32581 ggtcggcaac cacccaaccc ggactgactg gcacaatcgc aaaggccagc agcagcgagg
    32641 taaaggcaat catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt
    32701 cttctttaaa gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa
    32761 cacggttagg tccgtaacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca
    32821 tgaatgcccc gcaggtgaca accaccagca ggatcaccac cgctttgagg atggtcagca
    32881 ggatctcaat cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag
    32941 atgcgcgcca gccagcaccg gagcaatgcc ggacataccc atcggcaaac ccacctgccc
    33001 tgccgtcagc ccttcagcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa
    33061 ggagacgcga gcgcctgcgt tcacgcccaa cttcgcggca tccgctgggt tgagtttgat
    33121 gtacggctgc ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc
    33181 aaacaggtgg taatacggcg cgatacgcca tttcccgtcc tgcggctgga agcgtgccgg
    33241 tacgctggtg aagtaatcca gaccattttc gctggtttca aacagacgca cgcccggatc
    33301 gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt
    33361 ccagcccggc gcccaggcaa acggcacttg cgaacggtgc gcagtcggct ggttgttacc
    33421 ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct
    33481 gatattggcg cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg ccagtttctg
    33541 cccacgaata cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat
    33601 tttcgccaca acagcgtcaa tcacatggtc gagctgcgtc cagtccactt cacggctcag
    33661 cagggtgctg tgcagcgagt gtaaccagcg ccagctttcc agcatgacag ttttgctgtc
    33721 gtaataagca ggatcgtaaa cctggaagaa acgttgggcg cggccttcgt tgttgatcac
    33781 cgtaccgtcg ctttcagcaa agctggcagc agaaagtacc agatgggcgt tttccataat
    33841 cgctgtgcgt tgatgatcaa ccaccatcac cagcggcgct ttagccagcg cagcattcac
    33901 gcgggtagcg gaagcgtgac gatgcagatc gttttccagc accaccaccg cgtcggcgcg
    33961 tccggtttcc agttcggtca acgcttcttc aagcgaaccg ccacccataa tgcccagccc
    34021 catgctgttg acggaacggg caatcatggt gataccaacg tcagcgccgc gacctttcag
    34081 ggctttcgcg acgttagccg cagcctgaat cacctctatg ctaccggcgt tcgtcccgga
    34141 gataatcaac ggtttcttcg cacctgccag tgcctgcacg atgacgtcga ttttgctttg
    34201 cagctcaggt tcgataccgt caaccgctgg cgcagagtta tccagcgcat gggcgatggc
    34261 aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc
    34321 cagacgggtg tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt
    34381 gaggattgcc gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt
    34441 acctttcaca gcctgacgca ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc
    34501 gcccagcacc agtaccgcat cgtaagattc gatttcgcgc agagccggag tataaatgcc
    34561 gccttcacgc agcactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc
    34621 ggtgtagaag ttttcttcgc ccaccagttc acgcagcgca aagttgcttt ccacgctggc
    34681 acgcggagaa ccaataccga tcactttctt cgactgacgc agaatatctg ccgcgccctg
    34741 cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg
    34801 cggacgatcc ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta
    34861 gtggtttacc gtaccgttgt aacggttttc gatacgacgc agttcgccgt aacgttcacc
    34921 ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc
    34981 ccatttacgg ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat
    35041 ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta
    35101 gacgttgtcg tgcgcaccgt aaacgcccag atctgtaccg tcagcgtaat ctttgtagta
    35161 acgcacacag cggtagcagg cgatgcagcg gttcatttcg tgagagatga atggccccaa
    35221 atcctgatta cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat
    35281 cacggtcata tcctgaagat ggcagttacc gccttcttca cataccggac agtcgtgcgg
    35341 gtggttggtc attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc
    35401 aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg
    35461 cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc
    35521 cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag
    35581 caggttgtcc gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag
    35641 catgcttcca gttttctcgg tcagggaccg agcgttaatc gaaattcggt taccagcgct
    35701 ctttcagcag gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga
    35761 ttcccgcctc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac
    35821 caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg
    35881 tttcgatatc gcccggctga ccttcaccac gctccagcgc acgcagaatt ttcacgctcc
    35941 acggcagacc gtcgcggcac ggcgtacacc agccgcagga ttcacgggcg aaaaactctt
    36001 ccaggttacg caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg
    36061 tacccagacg gcttcccgct ttaccgatac tttcgaattc catcggcaga tcaaggtgcg
    36121 cttcggtcag gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat ttcagaccat
    36181 cacgcatacc accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt
    36241 cccacagtcc cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt
    36301 tacttttcga gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac
    36361 acagggtttc gacgttgttg acacaggtcg gtttgcccca tacgccggag gttgccggga
    36421 aggggggttt cgagcgtggg ttagcacgac gcccttccag ggagttgatt aacgctgttt
    36481 cttccccgca gatgtagcgc cctgccccgg tatggacgaa cagttcgaag tcgaaacctg
    36541 ttcccataat gtttttgcca agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca
    36601 gatttactgc cgcttcgata tattcgccac gcaggaagat gtagccacgg taagctttca
    36661 gcgcaaacgc ggagatgagc ataccttcca ccagcaggtg cggcagttgc tccatcagca
    36721 ggcggtcttt ataggtgcct ggctccattt catcggcgtt acacagcagg taacggatgt
    36781 tcatggattc gtctttcggc atcaggctcc atttcaggcc ggtagaaaag cccgcgccac
    36841 cgcgcccttt cagaccagcg tcttttacct gattaacgat ttcgtccgga gacagcccgg
    36901 tcagcgcctt acgcgcgcct tcgtaaccgt ttttgctgcg atattcgtcc agccacactg
    36961 gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt
    37021 ttttcattta taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt
    37081 atcctcatcg atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag
    37141 cagcgtaaag cgaccgtcaa atgtcgtttg ccctggtttg atgtttagct ttttctcgag
    37201 cgccgcctga ataccctggt aaccgttgat atgacagacc acgctgtcgc aataacggat
    37261 cacatggcgg ccaaccggct ggcggaagat ctggctgtag aacgttgcca caccttcgac
    37321 gtcgcttgcc ggaataccca gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca
    37381 gccacgctgc ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta
    37441 gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt
    37501 ttgtggttgt tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata
    37561 ctgcccagat aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc
    37621 tgcaaatgcg cataactcgg cgtgcggata cgagtgcgat aactcatggt gctaccgtcg
    37681 ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg
    37741 gcaggcatca ccggccccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt
    37801 tgcagcgtgc gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct
    37861 tccggcatgt tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact
    37921 ttcagcatta cgcgggtgta gcagtcagaa acaccaccac caaccgggat ttcaaagtcg
    37981 aagttttcat agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca
    38041 cgcaggcccg cgccagtggt gccccactcc agcgcctctt tcgcgccata ggcggcaacg
    38101 ccctgggaac gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga
    38161 cgtttcggca tccagtcgag gaactcacgc agcaggcgat cccagccgcg cggcaggtcg
    38221 tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc
    38281 accagatcgt aaattttctg acgatcggta aaggcgaaga acacgggcgt cattgcgccg
    38341 acgtcctgaa taaaggtcga gatgtacagc aggtggctgt tgatacggaa cagttcggag
    38401 agcataacgc gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc
    38461 gccagcacgt aaggcatttc gttaacgcag ccgccgaggt attcaatacg gtcggtatac
    38521 ggaatgtagc tgtgccagga ctggcgctcg cccattttct ccgcaccacg gtggtggtag
    38581 ccgatgtctg gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac gatacggaaa
    38641 gccccgtgcg ccgacgggtg gttcggaccg aggttgagga acatgaagtc ctcgttttcg
    38701 gtgccgcgct tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc
    38761 tgtttggctt tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta
    38821 cgcagcgggt gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg
    38881 tcgaaagtaa tgccaaacag atcccaggtt tcacgctcat accagttagc gttcgggaac
    38941 agtttggtga aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg
    39001 tcgcggttac gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac
    39061 ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa
    39121 ggtttcggca gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac
    39181 acaacgggaa ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg
    39241 cgcagttcgc caatcaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc
    39301 gcggttaagt cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag
    39361 taagcttcgc gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca
    39421 aattaaatct cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt
    39481 tcgcgctctg attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc
    39541 ggacgacgtt ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca
    39601 ggacgcggcg ggcagcccgg gatatacaca tcaaccggga tgaatttatc gacgccctgc
    39661 acaacggaat aaatatcgta cataccgccg gagttggcac aggcacccat tgagataacc
    39721 cattttggtt ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag
    39781 caggttcctg caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg
    39841 ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag
    39901 gaaagaccga agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg
    39961 agcttgccca taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc
    40021 tgcttttgca gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa
    40081 tccatcttaa tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc
    40141 atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa
    40201 accagacctg ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg
    40261 ctttcgcgga tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg
    40321 aagaacatgg ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag
    40381 tcgataccgg attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac
    40441 caaccgccta ccagcatcag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat
    40501 gcccagtgat gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa
    40561 gtagcgccag attcactgct cttcacggca gatggacgcc acatcgattc atggggagga
    40621 ataaaaaaaa ccttacaatc actgtagaaa ttcttttata cagctaattg atgtggtctt
    40681 ttactccttt ctataacctt ttgtcaactt taacaaaagt ttcttcacat tagtttacat
    40741 aatatcaaca ccattagcat ttaatgccct ttcaccccag atccttgacg actccaggat
    40801 aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt
    40861 gacaggaatt tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg
    40921 cttttgtcat tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt
    40981 agcaggtgat ttttttcagg cgattatttg tgcgttcggg acgtgaatct ctggtgattg
    41041 aaaaatgaac agttttgtac gttctgcact atgcgaggaa ggatttttac taaaaaaaag
    41101 ccgctggggt ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat
    41161 ctatcgtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga
    41221 gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc
    41281 tgggccagct cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc
    41341 ggcggtaagc catctactcc actcagcaca cgcaggtccg ggctcatcat ctcaaccggc
    41401 cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg
    41461 acataagcca gtcgccatgg aatatctgct ttattaagcg tcgccagcac catatcgcga
    41521 aacggacttg gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat
    41581 tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa
    41641 ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag
    41701 gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt
    41761 aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc
    41821 tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg
    41881 gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg
    41941 aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga
    42001 gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat
    42061 gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat
    42121 cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc
    42181 atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata
    42241 tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag
    42301 aatatatctt cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg
    42361 cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt
    42421 catacgcgaa cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat
    42481 ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg aattatctga
    42541 tgcaaagtta tgttatgtta ggcaaagtaa tcttctatta ttaataagca catcaaaacc
    42601 tttttgaata ttaaataata attaattagc atcatcctca ttcattaatt ccgcttaaca
    42661 atagtttcac accttgcccc cattacgaca cctcggaaat catcacgctg ttaaactctc
    42721 cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat tcttaaatga ggcattttca
    42781 cacaattatc ttacagataa aaaaaccata cttacaatta agaatcagaa caattcacta
    42841 tataacattg catgtaaagc atatacacct cattattttg tcattattaa gttattaaca
    42901 gcacaatcga gccttcccct ctggcaaaat cttattctgc agaccttcaa aacaccgtcc
    42961 tgggggagta cattgttcta agctgacttc cacggcaggg agtggcgata acagcaaaaa
    43021 aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt ctgttatgac
    43081 atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa caaggtactg
    43141 aaactgaaca tcggcaaccc agccccgttc ggttttgacg cgccagatga aatcctcgtt
    43201 gacgtgatac gcaacctgcc taccgctcaa gggtattgcg attccaaagg tctttactcc
    43261 gcgcgtaaag ccatcatgca gcactaccag gctcgtggca tgcgtgatgt taccgtggaa
    43321 gatatttaca tcggcaatgg tgtatcggag cttatcgttc aggcaatgca ggcattgctg
    43381 aacagcgggg acgaaatgtt ggttcctgca ccagattacc cactatggac cgcggcggtt
    43441 tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg aatcctctga ctggttcccg
    43501 gacctcgatg atattcgcgc taaaattacg cctcgtacgc gtgggatcgt tattatcaac
    43561 ccaaataacc caaccggcgc ggtatattcc aaagagcttt taatggagat tgtggagatt
    43621 gcacgtcagc ataatctcat tatcttcgcc gatgaaattt atgacaaaat tctttacgac
    43681 gacgctgagc atcactcaat tgcgccgctg gcacctgacc tgctgaccat tacctttaac
    43741 ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg ggtggatggt gttgaacggg
    43801 ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa tgctggcttc aatgcgcctg
    43861 tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc tgggtggtta tcagagcatc
    43921 agtgaattta ttacccctgg cggtcgtctt tatgagcagc gtaaccgcgc gtgggaactg
    43981 atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg gtgcgctgta tatgttcccg
    44041 aaaatcgacg ccaaacgctt taacattcac gacgatcaga aaatggtgct ggatttcctg
    44101 ttgcaggaaa aagttctgtt ggtgcaaggg acggcattca actggccgtg gccggatcac
    44161 ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag caagttcgcg
    44221 cgtttccttt ctggttatca tcagctgtaa tcttaatttc actgccggag attgcatccg
    44281 gcagcgttat cccgccacaa tgacctgatg atgtcatcat acgtaaggtc actatgaaac
    44341 agagccattt ttttgcccat ctctcccgcc tgaaactcat taaccgctgg ccgctaatgc
    44401 gcaacgtgct gacggaaaat gtgtccgaac acagtttgca ggtagcgatg gtcgcccatg
    44461 cgctggcagc tatcaaaaat cgaaaatttg gcggtaatgt caacgccgaa cgtatcgctt
    44521 tactggcgat gtaccacgat gcctcagaag tgctcaccgg cgatctccct actccggtga
    44581 aatacttcaa ttcgcaaatc gctcaggagt acaaggctat tgaaaaaatc gctcagcaaa
    44641 aactggtcga tatggttccg gaagagctgc gggatatctt tgcgccgtta attgacgagc
    44701 atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg tgtgcatatc
    44761 tgaaatgtct ggaagaactc gcggccggaa ataatgaatt cttgctggca aaaacgcgac
    44821 tggaagcgac gcttgaatcg cgtcgcagcc aggagatgga ctacttcatg gaagtatttg
    44881 ttcccagctt ccatctttcg ctcgatgaga ttagccagga ttcaccgctg taagcagccg
    44941 gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg
    45001 aaacagcatc gggatcatca ccacacaaac cgccatcaca ataatggtga acggtacccc
    45061 caacttcaca aagtcactga agctgtaatt tcccggacct aaaaccagtg tgttaacagg
    45121 tgaagaaacc ggtgtcataa aggcggcgga tgctgccatc gccacgacca tcgcgaatgg
    45181 ataaggcgac acccccatcg ttttggcagc agccagcgca atcggagcca tcaacaccgc
    45241 cgtcgcggta ttagagataa atagcccaat aaccgccgac aagacaaaca aacaacccag
    45301 catcatatgt ggcccgtaac caccgccaat gtccatcagc cctttcaccg ccagcgcgac
    45361 acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc ccaacgatca aaataatgct
    45421 cggccagtga atggatttat aggcgctttc agcatctata cagcggaatt tccccatcag
    45481 caggcaggcg atgatagcgg caacaggatt aggaatttca tctgtcagca ttaacgccac
    45541 cattagcacc agacagaaaa tggcatgggg tgcctggctg tgcgcgggcg atgcttcact
    45601 cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc tgtttggcca gcataccgat
    45661 cagtttccag ttacccacaa ccaggatgat atcgcccagc agcagaggct catccgccag
    45721 cgaaccttcc agcgccacgc cattgcgctt tagccccacc acattcagtc cgtagcgggt
    45781 acgaaaacca atttcgcgca ccgatttacc aatcagttct gactcaggaa ttaatgaaat
    45841 ctctgccatg cccacatcaa gggcctggtc agaaaaatac tcgccgcgca gtaccatcgg
    45901 ctccagcaat tgctcactac aaaattgccg gagatcgaca tcagccgcag acatatcaat
    45961 aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta acgttcacga taacgcgacg
    46021 aaaacgccgc cagcgttcaa caccgatgac gttagcgcca taacgctcac gtaatttgag
    46081 atcatccagc cgttgaccaa tcattggcga tccggggcga atagccagac gtcgcgcacg
    46141 cccggtcagt cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg
    46201 ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc actaacatat acaaaatacc
    46261 cagcaccagc acgaccaggc caataggtgt tacgctaaag aaactgaagc cgtgatagcc
    46321 ttcacgcagc aattcactgt tgactaccag gttcggcggc gtcgccacca gcgtcatcat
    46381 gccgcttatc agcccggcaa aactcaacgg catcatcaga cgtgacggcg aagtttgcat
    46441 acgcatggca acgcttaaca ccacgggaat aaagatagcg acaacgccgg ttgaactcat
    46501 aaacgcgcca agccccgcga cggtcagcat caacaaaacc aacattttga tttcactgtt
    46561 gcccgcaact ttgaccagcc atgttcccat tacggtggca acaccggtac ggaccaaacc
    46621 atcgccaata ataaacaagg cggcaatcag gacaacgtta ggatcagaaa agccggaaaa
    46681 tacttcgggg actgtcagcg ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac
    46741 cgcatccata cgcactctgc ccgtcgcaaa caagacaatg gcaacggcca gtaatgaaag
    46801 aacccaaatc aattcaccgt tcacaactta tccttgttaa ttgagggaga tgtcttgatt
    46861 ctgccataaa aaagccccga cgagacgggg ctaaatcatg atcaggtgtt tcactgaata
    46921 ataacatcgc catttggctg tttggtcaca gtaatttgct ccagactgtg gaggaccaaa
    46981 tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa ccgcaatgac atggcaaccc
    47041 gccgccaggc cagaaagcac gccagcggga gcatcttcca ccaccacaca ctcctgcggc
    47101 gcaagcccca gcagctgcgc gcctaacaga tacgcatcag gttctggttt tccgcgcttc
    47161 actcgctcag cggttacaaa cacctctggt gcgggaagcc cagctatttt atggcgcgct
    47221 cgcgctaccg gcatggagcc agaagtcaca atggcccacg gaatacctgc tttattcaaa
    47281 tgattgagta aggcgattgc ccccggaagc gcggtaatac cttcggtttc cgtggcctcg
    47341 atgtactcca gacgcgtaaa ttcggcggca atatcagcct cggatttgcc cgccataaaa
    47401 tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc ttccggcgct
    47461 aacccatgac gtctggccca gttgctccac gcccgttcta ccgcaggcag ggaatccacc
    47521 agcgttccat caagatcaaa cagaaaacct ttgcaccgca cgcgggcctc ctcaggcgtt
    47581 gataatttga ttaatttcgt tggcgctcaa atggtactga cgcgggcagg catgccacac
    47641 attaagcatg cgctgatatt tttcccacat tggcgtctgg gcgttaaaac cgtgagttcc
    47701 ggcgtcaaaa tgggtatagc gcccttccac attaaccata aagcggacat aaccgaggta
    47761 acgtgcttca gtggcggcgt caaagccgag gaaggtgaca cgacgttcat cgatggattg
    47821 ctggtcctgc aaattagacc aggaaacatg caacgcatga tacatctcca taatgtcgat
    47881 gatagtgcgg caggtttctt ctttcagctc gccaaactcg cgatccaatt cacgcatctg
    47941 taatccgtaa ccacgctcaa taattgtttg caggcgacgg taacgttcag catttgccgg
    48001 atcgagcata gtcatcatct tgtactgatt agacaaaata agacgttgcg cgttggtcat
    48061 ttccattgtt gactcctgta tcactctact acggtgaaaa aaaagaaggc tgagtatgcc
    48121 ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt ttatccagtt
    48181 gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg ccttcaacgg
    48241 gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac cagttgagga
    48301 tagcaggggg taatggcgtt acagaacgct tgcccagcca ccataatccc tgcatgggta
    48361 aacttaaggc gaacagcgca gtggcaactg ccggcccaag ctgaccgccc agggcaatct
    48421 gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa cgcgtcatct
    48481 tgataacgcg attttcgaca aagaccgggg caaggcgttt ttccagcggc cacgtctttg
    48541 agtaatgctg tccccggcga aacaagctaa aaaaattaac agaacgatta tccggcgttg
    48601 acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat tcacaactca
    48661 gcgggacaac gttcaaaaca ttttgtcttc catacccact atcaggtatc ctttagcagc
    48721 ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat tatgccattg
    48781 gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg caaaaaaaat
    48841 ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc tctatggctc
    48901 cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt aagttagtac
    48961 tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca gtaaatggtg
    49021 aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt atcaaatgga
    49081 aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac agcgaagcgc
    49141 tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg cagctgactg
    49201 ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta gtgatcgatg
    49261 agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctgcac aacccggctc
    49321 acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac aaaaacgttg
    49381 ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc tacgccctgc
    49441 cttacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc accagccact
    49501 tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca
    49561 tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt aaatgcgttg
    49621 acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt tctggtgata
    49681 tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt gacgcaatca
    49741 acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc agcgactgcc
    49801 gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact
    49861 gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt cgtctggacg
    49921 ctgttgtatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa ctgtctctgg
    49981 gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct gcacgtttcg
    50041 gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg
    50101 aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac accgccagct
    50161 cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag gataaaccgt
    50221 gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct
    50281 tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca aacctatcgc
    50341 tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc gtgcgaactc
    50401 ttccaccacg acggccgctg aaccgctgaa aatgagctac gttgaaggtc tgctttccag
    50461 caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta acaccaaaga
    50521 cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt ttgcccagtc
    50581 tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg
    50641 cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg
    50701 tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac cggttgatga
    50761 acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca aagctaaagt
    50821 aaacaatgtt gatccggcga agctgcaaga atccagcccg ctgccggttc tcggcgctgt
    50881 gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc acctgaatgc
    50941 gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc
    51001 acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg tgacttccgc
    51061 agaccgtcct gacgtgctgg tggccgcttg cctggcagcc atgaacggcg tagaaatcgg
    51121 tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg
    51181 tgctttcgct accggcctgc cggtatttat ggtgaacacc aacacctggc agacctctct
    51241 gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgaacgta tcgagaaagt
    51301 tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga ctgccacttc
    51361 tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat cagctgactg aacttgcgcg
    51421 caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg ttaaagcagc
    51481 cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat
    51541 caaccgtgtt gcagcgtctc agggtgtaga actgggtgca gggattgaaa tcgttgatcc
    51601 agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga acaaaggcat
    51661 gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta cgctgatgct
    51721 ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg caaacaccat
    51781 ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat cttccgtgtt
    51841 cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca acccggatcc
    51901 gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg cggccttcgg
    51961 tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg caggtagcga
    52021 cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg acctgatgat
    52081 cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat ccaaagcgcc
    52141 gaactctccg gttgcaggtc gcgctaccgt gttcatcttc ccggatctga acaccggtaa
    52201 caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc cgatgctgca
    52261 gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg atatcgtcta
    52321 caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc atcatccgca
    52381 gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt ttattctctt
    52441 aatctgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat gaattgcgcc
    52501 atttcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc
    52561 aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg tgattatctt
    52621 ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtggggt tgtttgacag
    52681 tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg tagatccaca
    52741 ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg tacagctgtt
    52801 cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaaggat taacctcagg
    52861 atcgaaatac gggacagctg ttggcatcat catgtttatg ctggtgattg gcggcgcgtt
    52921 tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta ttcgccatac
    52981 ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac ttggcggcgc
    53041 ggtatttggt atgggagaag aggccgtcgc ctttgccatt atcatcgcac cgctaatggt
    53101 ccggctgggc tatgacagta ttaccaccgt cctggtgacc tatattgcca cgcaaatcgg
    53161 ttttgccagt tcgtggatga acccgttttg tgtggtcgtt gctcagggga ttgccggcgt
    53221 tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca ctctgattgg
    53281 cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt
    53341 gcatgagtcc gaccgcttct ttcgtgaaaa gcaggctgat gttgaacaac gtccgtttac
    53401 ctttggtgac tggctggtat tgattgtcct gaccgccgta atggtctggg tgatttgggg
    53461 cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca ccatgggtct
    53521 ggtgattggc atcatcggcg ttgttttccg ccttaacggc atgacggtta ataccatggc
    53581 ttcatccttt accgaagggg cgcgaatgat gatcgcccct gccctgctgg tgggtttcgc
    53641 caaagggatt ttgctgctgg tcggtaatgg tgaagcaggt gatgccagcg tgttaaatac
    53701 catcctcaac agcattgcca atgccattag cggtctggac aacgctgtcg cggcctggtt
    53761 tatgttgctc ttccaggcgg tatttaattt ctttgtgaca tccggttctg gtcaggcggc
    53821 gttaaccatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc gtcaggttac
    53881 cgtgctggca ttccagtttg gtgatggctt cagccacatc atttacccaa cctcagcttc
    53941 gttaatggcg acgctcggtg tttgccgggt ggacttccgt aactggctga aggtgggcgc
    54001 gacactgctt ggactgctgt ttattatgtc cagcgtcgtg gtgatcggcg ctcagttgat
    54061 gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt ctgccgtttc
    54121 agtctctacg gcttcatttt tggcattacg cttcatccac aacgccagcg ctttcagcga
    54181 gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc agcaaacttc
    54241 actgacttca tcttcctgta gggcgaaggg accgtgagag acgcagctga acaatgcgcc
    54301 ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcagcaa aggggacacc
    54361 ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat tccagcagtt gctcatcggc
    54421 ctggaccaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt
    54481 acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg
    54541 acgcagacac tgtgcccgca tttgttcccg gctggcttgt gcaatgactt cgttctcttc
    54601 attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta tcaggaaacc
    54661 ttctttttct ggcgctctta cggcgcattt tgagttgtgg gtaaattacg gattaatcgc
    54721 gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt cattatccag
    54781 catgccataa ctcgccggat taccgccttt cggaatactc accgagccgg ggttgaagtg
    54841 gaaaatctcc ccccgctgtt ctgccactgg cagatgggta tgaccgtaca ccagcacatc
    54901 gttctggttt aaagcaggta gattttccgg gccaaaaaga tgaccatgcg tcaaaaacag
    54961 acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat gcagcagcat
    55021 ttgatccact tcgctgtcgc agttgccgcg cacagcgata accttatgtg ccacttcatt
    55081 aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac gcgggccgtg
    55141 attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa acaactccag
    55201 aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca gtttcatcac
    55261 tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc tatcagccag
    55321 attgcttcgc cgaaagtgca ataaaacgct ggacagacgc tcgctgccac tggaatgtcg
    55381 cataatccac caggcgttct ggcacctcat cgccatgtag taccaggcgg ttaatcatta
    55441 gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga cccagtgcta
    55501 acaaatgttc tgccatcgcg aacagtttct ctgcactggc ttttccctcg gccgttagtg
    55561 gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg atgggcatca
    55621 gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc acgattttct aaatcaagcg
    55681 gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc gcaatggcag
    55741 aagattcact caactcaaaa tcatcgattt gtaataacgg cacacggcgt gtctgaccgt
    55801 aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag gtcgatggtc ttgatatgaa
    55861 acgacaggcc tttttcctgc aacgccaccc aggcggataa cacataaggg gagaaaaagt
    55921 gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca ataaaggggg
    55981 ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact cacgtaagtc
    56041 gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg caatgatcga
    56101 tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg aagaagcaga
    56161 gcttgattat cgcttgatta aggtagacct ggggaaaggc ggtcagtttc gtccggaatt
    56221 tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc ctgccgatgg
    56281 cggcgaaccg ttaagcctct ttgagtctgg tgccattttg ttgtatctgg ctgagaaaac
    56341 aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc acattacagt ggttattctg
    56401 gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc acgcagcccc
    56461 ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc tttaccatgt
    56521 actgaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca gcattgcgga
    56581 tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc tcgcaatgta
    56641 tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg ggcaggcact
    56701 gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg ttctcgtgta
    56761 ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc cgctattatt
    56821 cgtataaaga accttcgttt acgtacgttt atcggaatta aggaagaaga aattaacaac
    56881 cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc gcgtaccagt
    56941 gaagatatta atgacgcgct gaactatcgc accgtcacca aaaacatcat tcaacatgta
    57001 gagaataacc gtttctcttt gctggaaaaa ttaactcagg atgtgctcga tatcgcacgt
    57061 gaacatcact gggtgacgta tgctgaagtg gagatcgata aactacacgc gctgcgctac
    57121 gccgattcgg tatccatgac cttgagctgg cagcgttaac cgccatatcg ggaggctgca
    57181 tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt ccgcgtttgc
    57241 tggagttggg ccatcaaatc acggtagtga cgcgtaaccc gcagaaagcc agttccgttc
    57301 tcggccctcg ggtgacacta tggcaagggc tagccgatca aagcaacctc aacggcgttg
    57361 atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact cacgagcaaa
    57421 aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat ttgattaatg
    57481 ccagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat tatggcgact
    57541 taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact cataaactct
    57601 gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga gtgtgcctgc
    57661 tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg ctgccaccgt
    57721 ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca gtatcttgcc tggattcaaa
    57781 tcgatgatat ggtcaacggc attctctggc tgctggataa cgagctgcgc gggccattta
    57841 atatggtttc gccctacccg atacgcaatg aacaatttgc ccatgcgctc ggtcatgcgc
    57901 tgcatcgccc ggctattttg cgcgtccccg caaccgccat tcggttgtta atgggcgaat
    57961 cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa gaagcgggtt
    58021 ttgcgtttcg ttggtacgat ttagaagagg cgctggcgga tgtcgttcgc tgatgtggtt
    58081 tactgcaaac atccgccagt taactcccgg tgttacaggg ttagtggctt tgcgctataa
    58141 gatcgtctgg cgatagtcgg gtcaccatca taactaactc tctgtctaaa cctctatcca
    58201 gcatctcctg agcaatacgc agggcttctt cgcgtttgcc cagaattaat ccatcattcc
    58261 gcccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    58321 gttctgctat ctcaccaata aatgcacgga aacgctgggc atctcccgtt tgtaatacat
    58381 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    58441 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    58501 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    58561 gaaaagccga tgaatatatt ttacgggcaa tcgcaggctc agcaaattca tcaagccagc
    58621 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    58681 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    58741 tgcgaaaagc cattaattct tccggcttac tttggtgctc tatcactaca taaatataac
    58801 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtcgt
    58861 catcaataaa actgtttggt tccagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    58921 gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc tggatggcgt aaaaaagatt
    58981 taaataccgc atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccat
    59041 caaatttaag atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta
    59101 tctttggaca gcgaattcag aagtattttt agcggactgc aacttttaca tttcagcgga
    59161 agctgactcg caaattcccc atgaaaatac tccggcagcg ttagccaccg gagaggaaat
    59221 tatttcagcg atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac
    59281 tgctccggcg cgccctcttc ttctattttc ccctgatgga ggaaaatgac gtgagtagaa
    59341 acatggcgag caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc
    59401 agttgctgca taatacgtaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt
    59461 tcatcaaaca gtaaaacttc cggttccatc gccagcgccc gcgcgataga aacacgctgt
    59521 tgctgaccgc cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact
    59581 tttgccagat acttcaccgc ccgctcgcgc gcttcctgct tgctcaggcc caacacctga
    59641 atcggcgctt ccatgacgtt ttccagcacc gtcatatggc tccagagatt gaagtgctgg
    59701 aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat ttttatcggc gactttgagt
    59761 tgaccgtctt tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgacccttca
    59821 ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat
    59881 ccgatgatgc ttattacatc tccggcattc gcttgcagtg agaccccttt cagcacttca
    59941 tgttcgccgt agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc
    60001 tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag
    60061 gctgatcagg acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa
    60121 aggttgatac gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc
    60181 cgtaaacgcc agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa
    60241 cgcaatacgc agcgcagaag gcaaaataat gcaacgatac attttaaaag tcgagaagcc
    60301 ataggctctg gcggcttcaa tttccccatg cggaaccgaa cggattgccc cagcaaaaat
    60361 ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc acggtacagt tcaggccact
    60421 gcggaagaaa gcgttaagga attcggttcc cttaacaatc tcaagcgtgt acatgccgga
    60481 atagaacacc agcaactgaa catacagcgg cgtaccacga aaaatatagg taaataacca
    60541 gattggaaac tggatgtatt tattactgga gacacgacca atcgccagaa acagcgccag
    60601 gactccgcct atcactaccg acaaaataag cagccacaga gtgatcgcca caccagtaaa
    60661 gcgataaccg tcggtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg
    60721 tcagccctct tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa
    60781 acagtggtga aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag
    60841 gtacttttgc ctgccagttg ggtggctttg accacatctt ccaggccgag taacgaaacc
    60901 agtgcggtag atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc
    60961 atcatcgccg gaaacatgat ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc
    61021 gccgcctcta tatgcccttt cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa
    61081 taagcaccgt agataaaacc gagagtaata ataccagcga ccatcggatc gatatcaatc
    61141 tgcccgacgc ccatcgcctc cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatc
    61201 agcagcatca acaccaagtc cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc
    61261 agccccgaaa gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca
    61321 gcgagcacta cagaactgat agccagctcc agcgtgacga gcgcaccctg taaaataaca
    61381 cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg
    61441 tctccctccg gttacccgaa gggagcgggc tttctttcac cgatggcgat taaccgccat
    61501 aaacatcaaa atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt
    61561 cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc
    61621 cggtccctac gccaaacagt ttttcatctt taacagacgg gccaccgaat ttgtaatctt
    61681 taccgacagg ttgtttgagg aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat
    61741 caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt
    61801 caatgccttt tggtgcccaa tgttcattac cgaacgtctc ctgggtggtg ccctgcaata
    61861 cgcctacccg tttgcctttc agcgactcga ctgtcggctg aatgtcggaa tttttcgcca
    61921 ccaccaaacg agaatcggca gcgtacagtt tgtcggtgaa ggctatttct tgctgacgtt
    61981 tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga
    62041 ttaacgcatc cagcggattt tcgacaaacg tacattgcgt attgatgcgt ttgcataatt
    62101 cctttgccag atcgatatcg aagccaacca gttcgccttg tgaatttttt gattcaaatg
    62161 gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg aatcgcagca aacgccgcag
    62221 ttgcgctgga gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct
    62281 caactgacag tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa
    62341 attgaccatc aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc
    62401 ttaagtatgc atttcaatgc accacgatag tgcaacaaac ctggcgtgca ccatgatgat
    62461 cattccatca ggtacagctt cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa
    62521 tactttttcg ccatcttgtc gtaggtgccg tcctgacgca gctcgccaag cgccttattg
    62581 aaggcagccg tcagttcagc atcatcttta cgtagcccta caccggtgcc atcaccgaag
    62641 tattttttgt cttttactga tgagccagca aaggcgaaat ctttaccagc aggttgcttg
    62701 aggaatcctt cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc
    62761 agatcggaat agaccaaatc ctggttggca taggccacca catccacgcc tttgctacgc
    62821 caggtctcgt tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct
    62881 ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg cagcaatcaa acgagaatct
    62941 gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac gtttatcggt aatggaaagc
    63001 gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca
    63061 ctggcaaccc aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata
    63121 tcaaagccaa caaaatcacc tttagcatct ttcgacgaga acggcgcata ggtggtatcg
    63181 gttccgatac gtaccgtctc cggtagcgcc gcatagctgg aagccgctgc ggagagaccg
    63241 actaacaaag acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct
    63301 tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat
    63361 cttgacgaca acagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtcc
    63421 tgaagttgaa gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa
    63481 caaaatgtcg aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag
    63541 ttatgcgccc tgccagcggg caaagagatc ttcaggaagg gttattgcaa actggtcaag
    63601 aacacgatta accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc
    63661 cggaacggga ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg
    63721 gcctaagtgc aatggtgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac
    63781 cacatctgcc gcacgggtca gtaagccatc agtatagcta tggacaatgc cggaaagggt
    63841 tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg aaagaaccgg aagagatgct
    63901 ggcggaaata tcgcgcgcat cgtgcgtgac atcagctaat gcctgcactt cgcgcaggga
    63961 aaaatccgtt tcgagggata aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc
    64021 gatatctgtg acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc
    64081 accgctgatg cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag
    64141 actttgcagt atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctcagcgaa
    64201 ggcatcatcc ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcactgctt
    64261 tggcgtcatc attacgtaac gtatcgagga aatcgaggta gccctgatca acatctttgg
    64321 tgacgtagac gccgttgaac accgagcatt caaactgctg gatatccgga ttttcagcgc
    64381 gaacggcgtc gatcagatcg ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct
    64441 ggcgaatttc atcaacttcg cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa
    64501 taccataaac gttcgggaag cgaatttccg gtgccgcaga agcgaggtac actttcttcg
    64561 ctccggcttc gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt
    64621 cgtcgaccag caggacgttt ttatcgcgga actcggcgcg gttggcattc agtttacggc
    64681 gcacggactt acgacgcagc tgctggcccg gcatgataaa ggtgcggcca acatagcggt
    64741 ttttaacgaa gccctggcgg tacggtttgc ccagaatacg agcaatttcc agcgcgatat
    64801 cacacgaggt ttctgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg
    64861 caattttctc gcccagtttc gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt
    64921 cgatgaacga gtccgggcga gcaaagtata catactcaaa caggcacgga ttgctgaccg
    64981 gattgtcagc acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc
    65041 ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag
    65101 cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa
    65161 tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat aatcatcgcc acacaggcat
    65221 atgcgccgcg gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca
    65281 gcgggtagtg gcggaagttg tccagctcgc tagcgaagat attaagcaga atttccgagt
    65341 cggaagtggt gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag
    65401 cgttggtcag attgccgttg tgggcaagcg taatgccata cggggagtta acgtaaaacg
    65461 gctgcgcttc agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca
    65521 tattgccctg caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg
    65581 ctttacgcaa acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac
    65641 cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg
    65701 cgataccgac aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt
    65761 acgctctggg caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa
    65821 aactgaactg cgggatcagc tgtgatttgc tccagtcttc gcttttcgac accccggtaa
    65881 aggagtcgag aaagaataga atggcggcaa caatcaacac accgcgcaac gcaccgaaac
    65941 agacgcccag cacccgatcg gtgcctgaca accccgtttt ctccaccagc tggcctatca
    66001 cgaagttcac gatagcacca acgatcaggg tagcgataaa tagtaccgcg atggcaatcc
    66061 catttcgaac cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat
    66121 gactggcaac aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc
    66181 cgcggatcag gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc
    66241 agaccatatg tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag
    66301 aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat caataaaaat ggcgctgaaa
    66361 aaatattcaa cgccatcgac tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg
    66421 cggcgtaaac gccttatccg acctacggtt ctaaccctgc ccagtcctga taagacgcgc
    66481 cagcgtcgca tcaggcaagg ccgtattaat tcggcgtata gcccattacc acgccactta
    66541 agccagaaag ttgcttcaac tcacccagcg aacctttcag cttatctttc gaggcatccg
    66601 gcccaaccag aatacgggta attttaccct gcactggcgt ggatggcgac gtataaaccc
    66661 gataaccggc accgcgcagc ttaccgacaa tctcatttac tttatcggca tttttcagcg
    66721 cacccagttg cacaacatag gctttacccg ttggcgctgc tttttcttcc acgaccggct
    66781 tcggttctgg cttcggcgca ggtggtgctt caaccttcgg tttaggcggc tccaccggtt
    66841 tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa
    66901 tagtggccgg atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc
    66961 cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg catcatatca ggctcatcac
    67021 gatcgcccgc tttcggcacc agcgggatag ccgcgaactc atcctgataa tgttttttct
    67081 gcccgtccag cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca
    67141 ctaaccgatt ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca
    67201 tgtgcgaccg tgtggaaaga accacacacc agcacggtgt cttccgcttt agcgtccgcc
    67261 attgcggcat cccatgcctg cgcaacgcta tcaaatgatt tgccgttacc caaatgctca
    67321 agcagttgtt ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca
    67381 accacgcttt tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata
    67441 accgccagca cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc
    67501 gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa
    67561 cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacttccagc
    67621 ccgctggcac gcagtgccgc cagcgctgtt gcggcattcg gttgcgggac aagcggcaac
    67681 ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa
    67741 tagttccact caacgccccg acgttgtaac agtgcacctt tttcctgcgc cacatcagca
    67801 atggtagaag gcatttccgg ctcaccgaca attgccggtt ttgcgctgcg aaagatgcct
    67861 gctttctcgc gaccaatact ttcgcgatct ggtcccagcc agtcggtatg atccagcgca
    67921 atgctggtta ctacagccac atcggcatcg acaatattgg ttgcgtccag acgaccgccc
    67981 agccctactt ccagaatcac tacgtcaagt tgtgcctgtt tgaacagcca caacgccgac
    68041 agcgtaccgt actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca
    68101 aaagaggcgg tgtgggccga ttccggcaat tcctgcccct gcacacgtac gcgctcggta
    68161 taacgcacca gatgcggtga actgtagacg cccactttgt accctgccgc catcagaatc
    68221 gactccagcg tacggcaggt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat
    68281 ggcgctggtt tcaggacgcc aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga
    68341 tcgatagttt tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg
    68401 gcttgaggag tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa
    68461 aagggcagag ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatccggt
    68521 accgggggta ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc
    68581 aacttcgcca gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg
    68641 atggcgcctt tctcgatcag gaattcactg cgctggaatc ctggcggcag tttttcgcga
    68701 acggtctgtt cgataacacg cggaccggca aagccaatta acgctttcgg ttcagcgatg
    68761 ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc
    68821 acggagatgt acggcaagcc gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc
    68881 atctgcatca gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag
    68941 atcagcgggc agttatcttc cagcgcctgc tcaacggcac gcacgaaacg tgcacccaca
    69001 acagacccca ttgaaccgcc cataaaggcg aactcgaatg ccgcagcgac aaccggcatt
    69061 ccatacagag tgcctttcat caccaccagc gcatcttttt cgccagtttc tttctgcgca
    69121 gatgccagac ggtctttata cttcttggag tcacgaaact tcagcacatc tttcggctca
    69181 agctcgctac ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc
    69241 gctgtcatac gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca
    69301 gcgcggtata aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc
    69361 gccttgcggg tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt
    69421 tctgtctgaa cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc
    69481 ccccaacaga ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa
    69541 agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac
    69601 gaataaattc gaaaacccca ggcaatattg aaacaaaaat aatgccaaca atcatcagct
    69661 taaggttgct ctgaatgaag gggagcgtgc cgaagaaata acccgcgtag gtaaaaatca
    69721 gtacccacaa cagtgcgccg atcacgttat aagcggcgaa atgacggtac gacatgtggc
    69781 ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg tacaaaacgg gcgagaataa
    69841 tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt atcaagataa ctgcgacgaa
    69901 agattttcga atccgggtta ctgaacagtt tgtcaccaaa taaccgtcca atggtatagt
    69961 tcactgcatc accgacaata gcggcaatca acataagcac caccatcata tggacattga
    70021 gatcgttagt ttccagcgac gccaatgcac cagcaacaaa cagcagcgaa tcaccaggca
    70081 ggaatggcgt taccaccagc cccgtttcgc agaacagaat caaaaacaga atggcataaa
    70141 cccagacgcc gtactccgcg accagctctg ccagatgcac gtcaatgtgc aggataaaat
    70201 cgatgaggaa ataaatcagg tccatatttg cctatgcctt gtactcgtca tctttcaggc
    70261 tgtaactacg ttggctacag cctgaaatgt tccgagcatt attctcgtta gtccgccaga
    70321 aatagcgggc ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc
    70381 agatacagcc cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc
    70441 aactctgcta tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca
    70501 atattcctga ccatatgatg tacaaaggca ttcgctttga tatctaccac cacataagga
    70561 ccgtgacgcg tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc
    70621 accgcacgga acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc
    70681 cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc
    70741 agccgatgat tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga
    70801 aaatcatcag gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt
    70861 acgcccaagg tccacgccgc gtctttgcgc tgcgcggtcg tttcgaaatg cactacctgc
    70921 ccggtaccgt gtacaccagc gtcagtacgc ccggcgcaga agacggtaat gggttcgttc
    70981 gccacctggg agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt
    71041 tgccagccgt aatacttact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc
    71101 ggttgttgct ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta
    71161 ctgccatcag cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tggacttgct
    71221 ccggcatacc gtagtcatta cgcacgcagc caacagaaag atgcggcgta cccgaagcat
    71281 cccctacctg agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa
    71341 acgcatcacg cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg
    71401 catgaccgta gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct
    71461 gcaggatttt gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat
    71521 ccggcaacaa cggcagcata ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa
    71581 tcggaatgcc gttgagcaat ttcgcactct gccccgctaa cgcatcgacc gcttttttgc
    71641 cctgggcgga ggctgaaatc aggctggtaa cactgatacg cgacaaaccg ccctgatcga
    71701 ttaacggttt cagtgccgcc agcagctggc tggtcagact gtctggtacg gcgatgacat
    71761 tccggttgcg gtaatcggtc agtacaaacg ggtttacttc cggcaccacc agcggtacgt
    71821 cgggttcgag agcaaacagt ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt
    71881 cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg
    71941 tccagtcgaa ttcagcggca tcctgcacgg tgattgtctt accaccaaag cgcagttgtt
    72001 cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag
    72061 ccagcgtttc aagcagggct tcgcccacag cgccagttgc gcccaggacg gcaatgttcc
    72121 agccttcaga catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca
    72181 gagagcatga agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca
    72241 cagcaatgat gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg
    72301 ctcaagatag tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag
    72361 ctgtgcgtca tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg
    72421 atcgagcggg ccatgcagcg taatgcgacc aaactctggc gcgggcagta atgtatccag
    72481 cgcaacgtgc tgttcatgcc caataaactt gctataggct tcaaacactt gcgtagtacc
    72541 gcgtgcttta ccttccaggg tataacctgc gatatgcggc gtgccgatat ccacttttgt
    72601 cagcaactcc acgttaagtt ccggttcgcc ttcccagaca tccagcacta cgcttaactt
    72661 ctggccttca ttcaggcagg tcagcaacgc agtattatcg acgactgcgc cacggcaggc
    72721 gttaatcaga atcgctcccg gcttcaggct acggatcagt ttttcatccg ccagatgcag
    72781 cgttttgtac ggcccatctt tgaagagtgg cgtatggaaa gtcagaatat ccgcatgctg
    72841 aactaattca tccagcgagc ggaaatcacc ctcatcccca cggtcagcgc gaggcggatc
    72901 gcaaagcaag gttttaatcc ctaacgcttc cagtcgcgct tgcaatcgac gtccaacgtt
    72961 accaacgccc acgatcccca ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag
    73021 catcagcagg gaggagaaaa catattccac caccgcaatc gcattacagc caggtgccgc
    73081 ggaaaaacca attcccgcct gctttaacca tgcttcatcg acatggtcgg tccctgctgt
    73141 ggcagtacca acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac
    73201 cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcg acggggattg gacgcccggg
    73261 aaccgcggtc acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc
    73321 aacaaggatt tttaaccctt tttcagtctc tgacaatcta aggctccttt attaacaaac
    73381 gcccatttgc aaactgctgt ttaatctgaa aaaacattca taatcataca gataacaaca
    73441 taaccattta gacgggcaat actgaaaaat tgttattcag tatcgccctt taagccgttt
    73501 caggctatat aaaattaatc ccccgtggac actgcgtgga cactcacgcc acctttcagc
    73561 ggattaaggg ccaccgcatc ctgcaggtaa tccggtgcaa aatgtgcata tgccattgtt
    73621 tgctgaatgg ttgcgtggcc aagaatcttc tgaagcgcaa taatgtttcc tccgttcatc
    73681 acaaaatggc tggcgaacgt atgccgcagc acatgtgcag cctggccttt tggcaaatcg
    73741 ggcttaactc ttttcagcgc caagcagaat tcccggtact tcacctcaaa caagccgcct
    73801 gtttctctgg ttttgatcgc ctcacaaact gcctgcgaaa ttggcactgt tctctttcgg
    73861 ccatttttgg tttcaagaaa cgttacacgg ttatgaacta tctgttcacc acgaagctta
    73921 caggcttcac tccagcgcgc ccccgtgctt aaacacaaaa gcgcaacacg ccagtaatcg
    73981 ccctccagtg tatcgagcaa taacgccact tccttctggg acaggaaagc catttctcgt
    74041 ggagatacat aaagaataga aattcccctt accggatgtt ctgcatccca gaggcctatt
    74101 ttcttcagta cggtaaacat tccggataac cgattcatgt atctgttagc agatgacggt
    74161 ttcaatccat cagctatctt ttgagaacgc cacgcgataa tttttagctt atcaagatcc
    74221 acagcctgca tatcagcgcc aagctcattg attatgttgc gcagttgttt tcggtcttct
    74281 tccgccttac gcctgtgctg gccgtgatac atccaccaca actcaagcaa atcatttagc
    74341 gttcgacgat cacggtagcc ctgtatatat tcccgctttt cagcgttcgc catgatgtag
    74401 cgttcagtgg ccaccgctac cgattttttg tcaaatacct tacgcacgcg ctttcccttg
    74461 cgtccgttcg gcctgatgtc cagcaaataa cgaccatctt cgagcttctt aatcgacatt
    74521 gcgaagccct ccaatgaacc gctctacaat ttctccagcc tctttccagc aataatcaga
    74581 ccagacaaaa agcaggtcta accagttttc tggcctcagc gggataattt ttggattgtt
    74641 tcggttgaaa cacgatctcc ctccgaattg caggaaccat caagagagag agccggagaa
    74701 atttgcccaa cctccggcat cgtttcatct gttgataacc aataagcata tttcttaaat
    74761 ttagggtgtt tcgtaacctt aagtaaggca ccctctgtta cttgcttccc cctcacctca
    74821 tagttagtta ccgtcccata aggcagccca acacaatccg caaactcctg gcgggtcata
    74881 ccttctgctt cacgaatcag gcgaaatttt tcacccatgc ttgacaaaga tgccatatcg
    74941 ggcatatcct ccatcgcaac atgccacatc gggcacaaac ataaaaacac tcaaataagc
    75001 cgatataagc cattttgagc cattcgaacg aattagggag attaccacaa tgagcgaatc
    75061 agagcttggg ggcttcattc aggtagcacc atatccactt gaagcggtgc catatcaact
    75121 attcgccaag atgatcggcc gcaaggaatc cacagtcaga accatgattg acgcagcaaa
    75181 gctaccgaca attgactttg tgaaaccagg ttcagtaaag acgcgtgcat cagaaaactg
    75241 ggtatatctg ccagcattta acgaaggcat gcgcaaagcg ttttttgagc aaccgaaaga
    75301 acgccgcgat gcatggttgt tgtggctggg gctttagtca taaatgacca gccatatcat
    75361 cagcgccatt ctgacccttg tttttattga gataggcgta atagcgatct atttgtttcg
    75421 taagttaacg ggacatgaag aacgctttat tgaactcagc attgaatata tcgccgctta
    75481 taccaaaggg cttttcccgg cagctattgg ggcgatgttt atagcgtttg ttatctggtt
    75541 tatcgggtga gaaacctgat aaaccacaac tactgcaatg agggtaatta tgtgtggcat
    75601 ggacaaattg gaccttatgt taattgtaat actatcaatt aacttcggtt acctcttaag
    75661 cggcgtaatt ctcatgtgcg gaggcaaacg aaaatgaacc agcagtccgc acaacgtgaa
    75721 aacaatgcga tgcgatttaa tcgcaaatat tttgagttcg gccgctatgc cggaataatt
    75781 aaatctgtac agagtcttta aaatgaaaca gcaacgtaat tcacgctttc gtaatggtgc
    75841 tgaacgtcac gctaaccgtt tcgctaccag tgcatcacgc agcaacatcc gctacagcct
    75901 gagtgataca cacgcaacgc cggatggcta cccagtaaaa caaatcggcg agcatgcctg
    75961 gctgattgag aaagctggaa tcgtggtcca caaatgccca cgcaatccgt ttactggaaa
    76021 ccgcattttt gctctaagca gcggcgaaaa tcagttcggg caggatttca cattatacga
    76081 agcactacgc acggttgatc gtctgcttcg cggacaaagt tttattaaac aggctgattt
    76141 ataacaggtg ctttatgacc aaagaccatg cacaaggtgt atttatccgt tttattgatt
    76201 ttcgcggtga actgttatta cgcgcatccg ctattgatgg agtggctccg gcgggtaaaa
    76261 acggagccga cgaagccact tacgtttatc tgaacggcac gcgactgctt gtggaacttc
    76321 cgtaccagac cgtacgagaa atcattagca aagctgaaaa agcgcgccag gctaatggca
    76381 atgaacccta tatcgaaatt atttgcatgg attcagaagc tgaaattcag aaagcagatt
    76441 aaagggtgtt gcaatgggca aagaatataa aacactcatc aacaaagcac ttgagcgttt
    76501 ttattttcgc ttaagtgcat caggctctca tgctgaacgt gcagcccgtg actcattgac
    76561 cagggcaatc cggagtctgt atgacgtggc tttttacgct gatgatctgg atgcacttaa
    76621 cgaactttcc gagctaatct gtgccgcaga atgcggggaa catattgaac cgtataagct
    76681 ggggaatatc gcatgagtat atttatctca tggcttgttc tgattatttc tgtggcctgc
    76741 gccattggga ttatgcgaat tattcattca gtaaaaaaga ttgaacgctt tttcactggc
    76801 gaataacaga gcaaataaaa ccacaggtta aataagaaaa tgtaaaaaca atccgcattc
    76861 gcggaggtat tcgcacacgt aaataacgga gatataaaat gaacacaaaa gaagaaggca
    76921 ttatcaacac attaaaaaaa atatcagaag cggaagatga aatggctaaa gatgccgtga
    76981 agcgtagcca acatatggca gcacttcacg cactgaccat tgcaaaaatc accgctgacg
    77041 cagccaaaat tattgaggaa cagagcaaag aaatcgacac tcttaaaaca cagtcaacag
    77101 ttgcagccat gaatccgtcc agcattggac gctgcattta tattcttggt tcagcaatga
    77161 tgccgcaata caccattatt gccgaactgc acggcaaata cctgataacg ccttaccaca
    77221 caaaagagtc tgagcttctg acaaatctcc gcctgataga acgctctcaa gctgtattca
    77281 ttgatgacgc gcaacgtgcc gtatttaacg catagggtta ctggacaaag ggggcgcaat
    77341 ggcaattaag catttttccg tcgttcgctt tacctccaga gggcgcgaat acgaggtcga
    77401 cgaacgcctg attaccacta tcgacaaaca tcgttcggaa aaggatgcgc accacatcta
    77461 cctcactgac ggcacttatt tctgcgccac caacgtggcg cgggtgaatc ttatccgaca
    77521 agtacaggag ccacgcagat gaccattctg gactacatcg ctactcatcc ggggtgtagc
    77581 ggcggagaga tcgccgcagc actgaatact ccaaccacag ccattaatgc tgagttacgc
    77641 caactttggc gcggcggctt agtcatcaga acaaaccgca gcacaggtgg tcgcgctcgc
    77701 aaaactggag gccaggcttc ttaccacgta aacccgatgc cgttcgggtg tagcaatcca
    77761 cttactcaca tgtttaacca gctactgaag gaagccagaa catgagcacc atcaaccacc
    77821 agaagctacg cgaactggca tttgccctgc aacgaatggc aactcctcaa aaattactgg
    77881 catttcgcgc aatgctctcg ccgtctgctg tgctggcact gctggatcag ctggagcacg
    77941 ccagaaccac ggctcctgcc attcgcctga cgctccatca tgaaatcgct gatttctgcg
    78001 caacgctggg ggcacctggc gaaccggaaa cgccggaagc aatgcagcaa gagctgctgc
    78061 aacgcattga caaggttttt gatttttttc tgaaccagta agaaaccaga acatgcacac
    78121 acaaaaaaac cgcttgccat gccgcaatcg gtcaggttac atttccgctg cacctcataa
    78181 aacgggtgcc gggattctca acccgataca gagcaaagcg cataaccgcg ccagcggttt
    78241 ttttgtgcgt actgtattgc cacgtctttt tcgcgtcaga attatggcgg ggcgtacggg
    78301 gccgacttcg gtcgggccgg attctttgct ctccggtgtt gagaaccctg tacgtctcgc
    78361 caccccgaga ttctcaactc tggatggtga gctatttcta tcaccgagca aagaggccac
    78421 accatggcaa accgcaaaca acagcgcgca tacgctgcgc gtcgtcacat ccagactgaa
    78481 atcaaccgta gacttttccg cgcatcacgc gtcgcgcaaa tcatgcacat caatatgctg
    78541 catgagcgca gccacgcgct atcgaacatc tattccgcct ctgttttcag ctatctggcg
    78601 gatgatctgc gcgagctgca acaactcttc cagcagcaaa acaaactcca ttaattcctg
    78661 ttccgggcct ttcctgcacc ttgcggcggg aggccttcgc acatctgtag taaagagaat
    78721 tgcagcatga ttgacgctca tgacttcaca agatgggtgc gcacacagga cacccgtctg
    78781 gctcccgttc ttcagggatt atttgatctc tacatccgtg gtcgtgacaa cagagcacgc
    78841 accacaaaac cggagaatgc agacaccctt tatttcacag tagacgactg ctaccgcgtg
    78901 gacttcactc cacacgggct ggcgttgcac tgcctgacac cacacggaga atcactgctg
    78961 gcgtattacg actccccggc ctccgtattt gcggcaatgc tggcgcatcg cactgctggc
    79021 gggtgtgcct cgctgagtga atacaccgct gaatttaacc gcctttctgc cctcttctcg
    79081 caggagtggc agcgcgtgac gggataccag ccatgaatga gtttgcatgg agctggaatg
    79141 aaccacggcc agccattgat ccggccagat ttacggagca caggcaggaa actgaaaccg
    79201 acctgcaacg cgccatccgt tactaccttg aggcagacaa aaaggctctg gaagaacagg
    79261 aagcgaagga ggaagccttt ttcgcacaat ccaccgtggg taaaaaactc atggcatccc
    79321 ttgaggaagc cggacagcgt gaaaagctgg cacaaagcat catcagcaag cgtcaggcaa
    79381 cagaacaaga cccggtggcc cgtgcttttg ccacactgaa ggtgcttccc gtttatctgc
    79441 gtgaacctct gagccgccac ctctctttcc tgcgcaaaaa acaggaagcc gatcgccaga
    79501 aaggcaaaaa gagctggcag gctgaacgct acgcgcgcgg aaccctgcgc aaaatattcg
    79561 aacgcctgga ccgcaccgac caccgctggc tgacaccggg ttatcgctcc cttgccggac
    79621 gcgaacgcct ggatgatttg ctttacctgc cgcagctcaa caagcaccag atacagacgc
    79681 tggccaccat gacggcggcg atgttcagca gcaccttcga aaaactctgc gatggctttg
    79741 gcgcgaccga tggtgagctg accatggagg tggcactgaa ggcgtatcag atgctggccc
    79801 gcatggcgtt acacctgcac gccatgcctc cacattatga cgcactgaca acagacaaag
    79861 accgcaagaa cgaaccggac acggaactgc tgccgggcgc aatcctccgc ctgacctgtg
    79921 cggaatggtg gaaacgcaaa ctgtggctgt tacgttgcga gtggcgggaa gaacaactcc
    79981 gcgccgcctg tctggtttcc agaaaaacat cgccctatct gagccaggac gcgttaagcg
    80041 agtttcgcgc acagcgcgag aaaacacgcg atttcctgaa aagtttcatg ctggaaaacg
    80101 aagacgggtt cacgattgat ctcgagacgg tgtattacgc gggagtaagt aacccggtcc
    80161 accgtaaagc agaaatgatg gccaccatga aggggctgga acttctggcc gaagcccgtg
    80221 gcgacagagc ggtgtttctg actgtcacct gcccgtcaaa ataccacgcc acaacagaga
    80281 acggtcatcc gaatcccaaa tggaacgggg ccaccatgcg cgactccagc gattacctgg
    80341 ttaacacgtt ttttgcggcg gtccgcaaaa aactgaaccg cgatggcctg cgctggtatg
    80401 gcatccgcac ggtggagcct caccatgacg gcaccgtgca ctggcatatg atggtctttg
    80461 cacatccgga cgagattgaa accatcgtgt cccatgtctg cgatattgcc attcaggaag
    80521 accgccacga gctgggcgat gacataactc cgcgttttaa ggctgagtac gtagacggct
    80581 caaaaggcac gccaaccagc tacatcgcca cctacatcgg aaagaacctg gacagccgcg
    80641 ccgtggatgg catcgacccg aaaacgggca agccacgcgt tgaccacgaa accggaaaat
    80701 caatggcaga gagcgtggaa cgcgccatcg gctgggcgcg ccttcaccgg gtccgccagt
    80761 tccagttctt tggcattccc tctcgtcagg tatggcgtga actccgccgc cttgccagcc
    80821 agatggcacg caacccggaa ggcccacaac ggctgaagga tgatgcaatg gatgcggtac
    80881 tcgctgccgc tgatgccggg tgttttgcca cctacattga gaaacagggc ggcgtacttg
    80941 ttccacgcaa agactacctg attcgcaccg cctacgacct cgcagatgag ctgaacgatt
    81001 acggcgaaca gagcgtacag atttacggga tctggtcacc actcatcggg gagtcttccc
    81061 gtgtgtgcac acatccggat aactggaagc tggtaagacg taaaccggaa gcggaagaca
    81121 gcgcccgcga aaatggtttt gaccttcagg gcggccctgc cgccccttgg actcgtggca
    81181 ataactgtcc ccgtgtacag gaaacagaca acaacgggac agaacagccg gaagaacggc
    81241 cagcaccgtg gccgcagctt cctgacggcg ttgacgtgaa cgaatgggtg cgctcactga
    81301 aacggcacga acgccgggcg ctgatgcgtt cgctgcgtga caaacaggca aaaaacagca
    81361 gtgatgaaat gcagaactgg acacagagcc gcaaacagcc acagcctttg cctgataacc
    81421 acgaattact cgctaaagaa tggcgggagt ctgctgaatc tctcggcctg catatcggtg
    81481 aacagcagat gcagcacctg ttacggggcg gcagtctgta cgttgacggc agcatcattg
    81541 caccgcaggg atttgaaatt gtacgcaaac cggatacccg cccggacagc cgaatcacgc
    81601 agctctggca acgcctgagc cgtaaccacg gcgtaagcag cacggagatc cgccataacc
    81661 cagtcgccag ctatctggaa caactagggg catcagaccc tgaagccgcc gcacgcctgg
    81721 catccacact tcagcaggac cagaacacca tgaaaacacc cgttaccgta ctttctgaca
    81781 tgctgcgcgc catccgcgac gcagagcacg aacagagaat cagtgaaaca actgaacgcg
    81841 cccgccgcaa agcagacctg ctgcggggtg acctgaccag tggaaacaaa aaacagacag
    81901 aaacgggatt cacaaatccc gtaaatgagc aaaaaacgcg ccgcgaaata tgaagtgcgc
    81961 acaaaacagg caaaagcggg atttcagaat cccgtaaacg gttaattaat caacataagg
    82021 aaaatcgaca tgaaaatttg tatcgacgac ggctccacca acatcaagct ggcatggact
    82081 gagaacggcg aacgccgcaa cgccatcagc ccgaacagct tcaagtcgga atggtctgcg
    82141 ccgttcggtg gcacgcagcc cgcgaactac atgcttgata gcgtgcgcta tggttttgat
    82201 ccggtcagcg atcgctttgt ccagacgact gacacgcaat atcagtacag tgatgtgaat
    82261 gtcattgcca ttcatcacgc gctggtcaaa tcaggcatca cgccacagga ggtggatgtg
    82321 gttgtcaccc tgccactgag cgaatatttc gacacaaacg cacagccgga catggccaac
    82381 atcaaccgca aaaaagcgaa cgttatgcgc ccggtggaat accagaacgg cgaagcattc
    82441 actatccgta atgtacgggt tatgcctgaa tccattccgg ctggctttaa ggcactggct
    82501 gacatgagtc cgtttgaatc cctgctgatt gtggatttag gcggaaccac gctggatgtg
    82561 gcaaaggttc agggacaact ggcaggtatc agccaggtgt tttgcgatcc acacgtaggc
    82621 gtttccctga tggccgatgc cgtactgtcg gtgatggcca ctaacggtat gcgtaccagt
    82681 caccacatcg ccaataccat tattgagcac cgtcacgatg aagcctggct gcgccagcac
    82741 attcacaatg acgcgcatta caacagcctg atggcggtta ttcgtgaaaa ggaggagaca
    82801 ctgaaacaac gagtgatccg cgcgctggcg ggtttttccg gttacgggcg ggtgatggtt
    82861 gtcggtggag gggcggagat tgtggcaccc gctatccgcg aagcctgcgg agttaatgcg
    82921 actttcatcg cggacggggt gccacaattt gctctggtta atgggctgta cgcaatgaac
    82981 aaggagtaaa ccaatgacga caccaaccag aagaataagt ttctatctga agccagctgc
    83041 cgtcaagaac gaaggagaag catgcgcctg gctggacagc cttacaccag aagcccgcaa
    83101 aagcggccaa cgcgtggctt ttctggccgg gctggcactt ctgaagacga atccggcaga
    83161 ggcttaccga ctggccgcat gggctgatga tgagatgtta cctgtgacac aaatcagctc
    83221 aaaaaagttt gaagcacagt ctgcaccagt ggctaagata accagccaga tggctgggaa
    83281 tatccgggcg ttatttcctg agtaaaagca tctgcacgaa aaatgctcac gtttatagaa
    83341 acagtatcat tccatttggc acacttactt caataatcga tctgttaaac aaatagattg
    83401 tctattatct atcgattaaa acgatcaatt atcttgacag taatgtgcct ttttgtaaga
    83461 tcgttcgcat tgtgagcgac aagataattg cgcggcattg tccatgcaaa acgccccaat
    83521 agcagcaact attggggcgt aaaactcggt cggactcact taacctgata tgcgtgcctt
    83581 ccgaagtaaa tcaaaatgtg cgtcgtatat tttattgctt acacgcacct ctgtaaaggc
    83641 acgcatattt ttcctatgag gtaaataaag tgcgggctaa aacaggtatt tgcaagaacc
    83701 ctcatcgtta taacccaaca tttctgtctc tccctgaata tcaaggacaa gaaggtcggc
    83761 acaaatgcgc tgcctgtgct tttgagctag gcataaaaga tgcgcttgaa ggacgcgcta
    83821 tggctcagaa tgatttagtt ttagctaaca ttccgtttag ccaggctgga actgtaaggc
    83881 acagagatgc ctatgaagca tatgttcgtg gttggcgatt aatacacagc aacaattgat
    83941 acttttgaag cgccgataag gcgctttttt ttgtttgcac gatagtgcac aagtttgcac
    84001 aatttttttg aacgactttt tgtacttccg gcccgcatgg cggctggatc cgtcaaggat
    84061 ccgtgcgtgc acaaaaaaat gcgttttttc tgcgcgcagg tgacggggga acagcccgcg
    84121 tttcaggggg taaatagcgt tcccttaacg atgtcgcagc gacacgacag aatggccgta
    84181 tttctcacgc tgagcgtgaa aaatacgtga gggattctgg tttgatgggg tgaaaggtaa
    84241 ggccgtcaaa atcgcactga ggcagcgaga acatgcagtc agcgcagtgg gattgcgtaa
    84301 gagtctgacc gtcgatgatg gcaataaaca ggaaagcgtc gtgaaattat ctgactgata
    84361 caggagctgg agagtcgggg cataaatttt ttatgccccg gcgaagcagc agacaagcga
    84421 agcgcgtcag gatgtgggct gggtatctaa cagtgcgtaa gggttaaagc ggatcacctc
    84481 ttcgccaagc cagtcattga tgtgcttcat ggcctccatg acgggcatca gctcgttaat
    84541 tgcgtaaacc cgcgctgcct tctccacatc accaaacgca cttttttcgc ccggcatcgc
    84601 ccccatcagt tgcggcggaa cgcggtgcgc agccagcaca tcatcacggg atgccgcctt
    84661 aacattcatg aactcatctt ttgcggtgat ctgctggaac ggcaaaattt gcaccccctc
    84721 tttgcccccg ttgggcgcat gaatgagcac gtttttaaac gcaccgccac cacgtgcacc
    84781 ctgtagcgtt tctttcaggg agtccatgct ttcgcggttt acctgcgctg caccgatgta
    84841 gatgatgcac ccggcgtggg atccgttgtc gtaatacagt tttctgaaca tgtccgccga
    84901 atgagacagg ctggccgaga gtaatgcgcc gagatattcc ggcatgccgt agatttcctg
    84961 gttaatgtca ggattcatca ggtggcacac tttgccaggg cgaaactgga acgcatcctt
    85021 gccatcctgc acataccacc atgattcaag atcgctaccg cgtcgcatgt atttcgccag
    85081 tgc
//
LOCUS       DAFATA010000008        77005 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFATA010000008 DAFATA010000000
VERSION     DAFATA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.008"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            423..1376
                     /gene="ompT"
                     /locus_tag="LGJ20_000831"
     CDS             423..1376
                     /gene="ompT"
                     /locus_tag="LGJ20_000831"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HBK2384949.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1563..3047
                     /locus_tag="LGJ20_000832"
     CDS             1563..3047
                     /locus_tag="LGJ20_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2384950.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHYLITPQKIENIYCPGNSEAEIQ"
     gene            complement(3306..3536)
                     /locus_tag="LGJ20_000833"
                     /pseudo
     CDS             complement(3306..3536)
                     /locus_tag="LGJ20_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            3593..4261
                     /locus_tag="LGJ20_000834"
     CDS             3593..4261
                     /locus_tag="LGJ20_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK2384951.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            4627..4740
                     /locus_tag="LGJ20_000835"
     CDS             4627..4740
                     /locus_tag="LGJ20_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384952.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            4809..5042
                     /gene="ydfK"
                     /locus_tag="LGJ20_000836"
     CDS             4809..5042
                     /gene="ydfK"
                     /locus_tag="LGJ20_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HBK2384953.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            5359..5949
                     /locus_tag="LGJ20_000837"
     CDS             5359..5949
                     /locus_tag="LGJ20_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2384954.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(6047..6622)
                     /locus_tag="LGJ20_000838"
     CDS             complement(6047..6622)
                     /locus_tag="LGJ20_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2384955.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(6622..9582)
                     /locus_tag="LGJ20_000839"
     CDS             complement(6622..9582)
                     /locus_tag="LGJ20_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384956.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVTRNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQEASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSATAAGNSVKAAKT
                     SETNARSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNST
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMST
                     GWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
     gene            complement(9647..10246)
                     /locus_tag="LGJ20_000840"
     CDS             complement(9647..10246)
                     /locus_tag="LGJ20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBK2384957.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(10317..13730)
                     /locus_tag="LGJ20_000841"
     CDS             complement(10317..13730)
                     /locus_tag="LGJ20_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBK2384958.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQCCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTVNENCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTEIPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            complement(13791..14423)
                     /locus_tag="LGJ20_000842"
     CDS             complement(13791..14423)
                     /locus_tag="LGJ20_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBK2384959.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGTGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(14360..15103)
                     /locus_tag="LGJ20_000843"
     CDS             complement(14360..15103)
                     /locus_tag="LGJ20_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384960.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            complement(15109..15807)
                     /locus_tag="LGJ20_000844"
     CDS             complement(15109..15807)
                     /locus_tag="LGJ20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBK2384961.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLIGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(15807..16136)
                     /locus_tag="LGJ20_000845"
     CDS             complement(15807..16136)
                     /locus_tag="LGJ20_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384962.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(16133..18712)
                     /locus_tag="LGJ20_000846"
     CDS             complement(16133..18712)
                     /locus_tag="LGJ20_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2384963.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            complement(18705..19139)
                     /locus_tag="LGJ20_000847"
     CDS             complement(18705..19139)
                     /locus_tag="LGJ20_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBK2384964.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(19121..19543)
                     /locus_tag="LGJ20_000848"
     CDS             complement(19121..19543)
                     /locus_tag="LGJ20_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBK2384965.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQTEDAGPAEPVSAGKCSMVS"
     gene            complement(19559..20299)
                     /locus_tag="LGJ20_000849"
     CDS             complement(19559..20299)
                     /locus_tag="LGJ20_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384966.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            complement(20307..20702)
                     /locus_tag="LGJ20_000850"
     CDS             complement(20307..20702)
                     /locus_tag="LGJ20_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384967.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(20699..21277)
                     /locus_tag="LGJ20_000851"
     CDS             complement(20699..21277)
                     /locus_tag="LGJ20_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2384968.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(21289..21642)
                     /locus_tag="LGJ20_000852"
     CDS             complement(21289..21642)
                     /locus_tag="LGJ20_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBK2384969.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(21654..22049)
                     /locus_tag="LGJ20_000853"
     CDS             complement(21654..22049)
                     /locus_tag="LGJ20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBK2384970.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            complement(22091..23116)
                     /locus_tag="LGJ20_000854"
     CDS             complement(22091..23116)
                     /locus_tag="LGJ20_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBK2384971.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(23172..23504)
                     /locus_tag="LGJ20_000855"
     CDS             complement(23172..23504)
                     /locus_tag="LGJ20_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBK2384972.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(23514..24833)
                     /locus_tag="LGJ20_000856"
     CDS             complement(23514..24833)
                     /locus_tag="LGJ20_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBK2384973.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(24814..26415)
                     /locus_tag="LGJ20_000857"
     CDS             complement(24814..26415)
                     /locus_tag="LGJ20_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2384974.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMISPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(26412..26618)
                     /locus_tag="LGJ20_000858"
     CDS             complement(26412..26618)
                     /locus_tag="LGJ20_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HBK2384975.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(26615..28540)
                     /locus_tag="LGJ20_000859"
     CDS             complement(26615..28540)
                     /locus_tag="LGJ20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBK2384976.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(28515..29060)
                     /locus_tag="LGJ20_000860"
     CDS             complement(28515..29060)
                     /locus_tag="LGJ20_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBK2384977.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            29449..29643
                     /locus_tag="LGJ20_000861"
     CDS             29449..29643
                     /locus_tag="LGJ20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBK2384978.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            30003..30296
                     /gene="iss"
                     /locus_tag="LGJ20_000862"
     CDS             30003..30296
                     /gene="iss"
                     /locus_tag="LGJ20_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HBK2384979.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(30387..30569)
                     /gene="rzoD"
                     /locus_tag="LGJ20_000863"
     CDS             complement(30387..30569)
                     /gene="rzoD"
                     /locus_tag="LGJ20_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HBK2384980.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(30786..31283)
                     /gene="rrrD"
                     /locus_tag="LGJ20_000864"
     CDS             complement(30786..31283)
                     /gene="rrrD"
                     /locus_tag="LGJ20_000864"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBK2384981.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(31283..31498)
                     /gene="essD"
                     /locus_tag="LGJ20_000865"
     CDS             complement(31283..31498)
                     /gene="essD"
                     /locus_tag="LGJ20_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBK2384982.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            32088..33185
                     /gene="ompC"
                     /locus_tag="LGJ20_000866"
     CDS             32088..33185
                     /gene="ompC"
                     /locus_tag="LGJ20_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBK2384983.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(33375..33758)
                     /gene="quuD"
                     /locus_tag="LGJ20_000867"
     CDS             complement(33375..33758)
                     /gene="quuD"
                     /locus_tag="LGJ20_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HBK2384984.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(33877..34239)
                     /locus_tag="LGJ20_000868"
     CDS             complement(33877..34239)
                     /locus_tag="LGJ20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBK2384985.1"
                     /translation="MNTYHITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDVQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            complement(34236..34526)
                     /locus_tag="LGJ20_000869"
     CDS             complement(34236..34526)
                     /locus_tag="LGJ20_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBK2384986.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAYIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(34519..34689)
                     /locus_tag="LGJ20_000870"
     CDS             complement(34519..34689)
                     /locus_tag="LGJ20_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384987.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(34689..35144)
                     /gene="ybcN"
                     /locus_tag="LGJ20_000871"
     CDS             complement(34689..35144)
                     /gene="ybcN"
                     /locus_tag="LGJ20_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HBK2384988.1"
                     /translation="MNLSQDGIKLHRGNFTAIGQQIQPYLEEGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(35141..35242)
                     /locus_tag="LGJ20_000872"
     CDS             complement(35141..35242)
                     /locus_tag="LGJ20_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2384989.1"
                     /translation="MRTHNPNSLLPSQMQKCTCNSLHLTFDLCGGEA"
     gene            complement(35876..37849)
                     /locus_tag="LGJ20_000873"
     CDS             complement(35876..37849)
                     /locus_tag="LGJ20_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBK2384990.1"
                     /translation="MEPMITFMECLGIYMKIRPQQHLINILTSTKQHHPNFTLFLGAG
                     ASISSGVDSAGGMIRRWRDAYTLMYGEDALKKQVWYDKDNEYSELFEALYDQPTQRRE
                     FIESCITAAKPSWGYVYLTNLLDKGHFNTIFTTNFDDLVNEACFTFSNNLRPIVCAHD
                     SSINSIRLTTARPKIIKLHGDFLFDNIKNTIRELESLEDNMRAKFRQFATEFGMIVIG
                     YSGHDRSIMDTLNTLLHSGSCFPHGIYWCIRDSDTEKLSEQLKNLARFPHFHLIKING
                     FDEFMAELHYALGCNLQQEVVEPYSALSNKLDRYFSIAEEDDADVQHEIIKRDMNNLA
                     DHVRRVNTVSQFVKKIKVLLSEKDSSLDDNSITKSLEIMLKETKFAGSPEGIHFYNTP
                     NYLIANSAFRAGEYNECVEYATKHLANGKSLETEVLILRSYIHLDNDSEIQKSIERIT
                     SYNKMNDGEVGKLVNGLVDLMDAKKFKIANSLMDILESRTIPSKHKQLLLINRVLCNK
                     LQKQPLSAKQQQELEEVLNEVIDKNEAWLALGAAILLEKWDIAEEVLSALDETEIVTI
                     LTQSMPIFDLISTEFYEKVKLVAIERGYNVSFEEETELPEVVPAEDETQSLSPDVREI
                     PTDKKKAITHKSADNDEAFDGLRTGEESISKPA"
     gene            complement(37967..38668)
                     /locus_tag="LGJ20_000874"
     CDS             complement(37967..38668)
                     /locus_tag="LGJ20_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBK2384991.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQLVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREETSVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARA"
     gene            complement(38665..39684)
                     /locus_tag="LGJ20_000875"
     CDS             complement(38665..39684)
                     /locus_tag="LGJ20_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK2384992.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKRLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSRWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWI
                     YGVDL"
     gene            complement(39681..40220)
                     /locus_tag="LGJ20_000876"
     CDS             complement(39681..40220)
                     /locus_tag="LGJ20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HBK2384993.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSYMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(40290..40520)
                     /locus_tag="LGJ20_000877"
     CDS             complement(40290..40520)
                     /locus_tag="LGJ20_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2384994.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            40625..41314
                     /locus_tag="LGJ20_000878"
     CDS             40625..41314
                     /locus_tag="LGJ20_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2384995.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            41910..42116
                     /locus_tag="LGJ20_000879"
     CDS             41910..42116
                     /locus_tag="LGJ20_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBK2384996.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            42191..42487
                     /locus_tag="LGJ20_000880"
     CDS             42191..42487
                     /locus_tag="LGJ20_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBK2384997.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            42493..43278
                     /gene="bet"
                     /locus_tag="LGJ20_000881"
     CDS             42493..43278
                     /gene="bet"
                     /locus_tag="LGJ20_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBK2384998.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            43275..43955
                     /locus_tag="LGJ20_000882"
     CDS             43275..43955
                     /locus_tag="LGJ20_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBK2384999.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVYNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            43952..44113
                     /locus_tag="LGJ20_000883"
     CDS             43952..44113
                     /locus_tag="LGJ20_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HBK2385000.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGLYD"
     gene            44106..44663
                     /locus_tag="LGJ20_000884"
     CDS             44106..44663
                     /locus_tag="LGJ20_000884"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HBK2385001.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            44674..44955
                     /locus_tag="LGJ20_000885"
     CDS             44674..44955
                     /locus_tag="LGJ20_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385002.1"
                     /translation="MHFSGSGLHILCAYACRHGTCSMTPQHENALRSIARQANSEIKK
                     ARQHFPDKNVDDICRSVLKKHRETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            45054..45272
                     /locus_tag="LGJ20_000886"
     CDS             45054..45272
                     /locus_tag="LGJ20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBK2385003.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            45320..45598
                     /locus_tag="LGJ20_000887"
     CDS             45320..45598
                     /locus_tag="LGJ20_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBK2385004.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAENIKKLRDMRSKSRGNHGIIQPHTR"
     gene            45797..46960
                     /locus_tag="LGJ20_000888"
     CDS             45797..46960
                     /locus_tag="LGJ20_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2385005.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKELPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGITEDIKKA"
     gene            complement(46976..47052)
                     /locus_tag="LGJ20_000889"
     tRNA            complement(46976..47052)
                     /locus_tag="LGJ20_000889"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47016..47018),aa:Arg,seq:tct)
     gene            47295..47927
                     /gene="fimZ"
                     /locus_tag="LGJ20_000890"
     CDS             47295..47927
                     /gene="fimZ"
                     /locus_tag="LGJ20_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HBK2385006.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(47930..48445)
                     /gene="sfmF"
                     /locus_tag="LGJ20_000891"
     CDS             complement(47930..48445)
                     /gene="sfmF"
                     /locus_tag="LGJ20_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBK2385007.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(48456..49463)
                     /gene="fimH"
                     /locus_tag="LGJ20_000892"
     CDS             complement(48456..49463)
                     /gene="fimH"
                     /locus_tag="LGJ20_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBK2385008.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(49476..52085)
                     /locus_tag="LGJ20_000893"
     CDS             complement(49476..52085)
                     /locus_tag="LGJ20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBK2385009.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(52116..52808)
                     /gene="fimC"
                     /locus_tag="LGJ20_000894"
     CDS             complement(52116..52808)
                     /gene="fimC"
                     /locus_tag="LGJ20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBK2385010.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(53028..53570)
                     /gene="fimA"
                     /locus_tag="LGJ20_000895"
     CDS             complement(53028..53570)
                     /gene="fimA"
                     /locus_tag="LGJ20_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBK2385011.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRIDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            54051..54917
                     /gene="folD"
                     /locus_tag="LGJ20_000896"
     CDS             54051..54917
                     /gene="folD"
                     /locus_tag="LGJ20_000896"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBK2385012.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            54919..55131
                     /gene="ybcJ"
                     /locus_tag="LGJ20_000897"
     CDS             54919..55131
                     /gene="ybcJ"
                     /locus_tag="LGJ20_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBK2385013.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            55239..55760
                     /locus_tag="LGJ20_000898"
     CDS             55239..55760
                     /locus_tag="LGJ20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2385014.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(55796..57181)
                     /gene="cysS"
                     /locus_tag="LGJ20_000899"
     CDS             complement(55796..57181)
                     /gene="cysS"
                     /locus_tag="LGJ20_000899"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK2385015.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            57355..57849
                     /gene="ppiB"
                     /locus_tag="LGJ20_000900"
     CDS             57355..57849
                     /gene="ppiB"
                     /locus_tag="LGJ20_000900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBK2385016.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            57852..58574
                     /gene="lpxH"
                     /locus_tag="LGJ20_000901"
     CDS             57852..58574
                     /gene="lpxH"
                     /locus_tag="LGJ20_000901"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBK2385017.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            58692..59201
                     /gene="purE"
                     /locus_tag="LGJ20_000902"
     CDS             58692..59201
                     /gene="purE"
                     /locus_tag="LGJ20_000902"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK2385018.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            59198..60265
                     /gene="purK"
                     /locus_tag="LGJ20_000903"
     CDS             59198..60265
                     /gene="purK"
                     /locus_tag="LGJ20_000903"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK2385019.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(60402..61295)
                     /locus_tag="LGJ20_000904"
     CDS             complement(60402..61295)
                     /locus_tag="LGJ20_000904"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK2385020.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKKVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEDTLAGEAGTCISL"
     gene            complement(61292..62107)
                     /locus_tag="LGJ20_000905"
     CDS             complement(61292..62107)
                     /locus_tag="LGJ20_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBK2385021.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(62118..63377)
                     /locus_tag="LGJ20_000906"
     CDS             complement(62118..63377)
                     /locus_tag="LGJ20_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBK2385022.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(63387..65054)
                     /locus_tag="LGJ20_000907"
     CDS             complement(63387..65054)
                     /locus_tag="LGJ20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBK2385023.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            65371..66420
                     /gene="allD"
                     /locus_tag="LGJ20_000908"
     CDS             65371..66420
                     /gene="allD"
                     /locus_tag="LGJ20_000908"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HBK2385024.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            66442..67677
                     /gene="allC"
                     /locus_tag="LGJ20_000909"
     CDS             66442..67677
                     /gene="allC"
                     /locus_tag="LGJ20_000909"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBK2385025.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            67688..68473
                     /gene="allE"
                     /locus_tag="LGJ20_000910"
     CDS             67688..68473
                     /gene="allE"
                     /locus_tag="LGJ20_000910"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBK2385026.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(68602..69747)
                     /gene="glxK"
                     /locus_tag="LGJ20_000911"
     CDS             complement(68602..69747)
                     /gene="glxK"
                     /locus_tag="LGJ20_000911"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HBK2385027.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(69769..71070)
                     /locus_tag="LGJ20_000912"
     CDS             complement(69769..71070)
                     /locus_tag="LGJ20_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBK2385028.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(71127..72488)
                     /gene="allB"
                     /locus_tag="LGJ20_000913"
     CDS             complement(71127..72488)
                     /gene="allB"
                     /locus_tag="LGJ20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HBK2385029.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(72548..74002)
                     /locus_tag="LGJ20_000914"
     CDS             complement(72548..74002)
                     /locus_tag="LGJ20_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HBK2385030.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFHEQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(74171..75049)
                     /gene="glxR"
                     /locus_tag="LGJ20_000915"
     CDS             complement(74171..75049)
                     /gene="glxR"
                     /locus_tag="LGJ20_000915"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBK2385031.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(75149..75925)
                     /gene="hyi"
                     /locus_tag="LGJ20_000916"
     CDS             complement(75149..75925)
                     /gene="hyi"
                     /locus_tag="LGJ20_000916"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBK2385032.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(75938..>77005)
                     /gene="gcl"
                     /locus_tag="LGJ20_000917"
     CDS             complement(75938..>77005)
                     /gene="gcl"
                     /locus_tag="LGJ20_000917"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBK2385033.1"
                     /translation="MGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFA
                     NRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAG
                     RLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQI
                     AAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEEL
                     AVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKV
                     AEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSELDNVME
                     FEDIADNAADAPTETCFMHYE"
BASE COUNT        19106 a        20583 c        18233 g        19083 t
ORIGIN      
        1 tcctagaacg acattttaag tcaacaactt accgcgctat ctctgcgctc acacgtccca
       61 ctacctcaaa acatgtaaag ccttgcaagc cattgcgagg ccttatgtgt ctcagttttg
      121 tccctctttt ttgtactaaa aaacatagta attgaggata aacctcatgc tattttcgct
      181 tatatgcctc taaaggcatg gcacttaaat agataaaagc accacaaaag cataaaaaaa
      241 ccacacagta aaaccgcaat atgaaacaat aacagataat taaaccaaaa acagatagcg
      301 cattgtgata atcattcaat actaaacaaa atataaacag tggagcaata tgtaattgac
      361 tcattaagtt agatataaaa aatacatatt caatcattaa aacgattgaa tggagaactt
      421 ttatgcgggc gaaacttctg ggaatagtcc tgacaacccc tattgcgatc agctcttttg
      481 cttctaccga gactttatcg tttactcctg acaacataaa tgcggacatt agtcttggaa
      541 ctctgagcgg aaaaacaaaa gagcgcgttt atctagctga agaaggaggc cgaaaagtca
      601 gtcaactcga ctggaaattc aataacgctg caattattaa aggtgcaatt aattgggatt
      661 tgatgcccca gatatctatt ggggctgcgg gctggactac tctcggcagc cgaggtggca
      721 atatggtcga tcaggactgg atggattcca gtaaccccgg aacctggaca gatgaaagta
      781 ggcaccctga tacacaactc aattatgcca acgaatttga tctgaatatc aaaggctggc
      841 tcctcaacga acccaattac cgcctgggac tcatggccgg atatcaggaa agccgttata
      901 gctttacagc cagaggtggt tcctatatct acagttctga ggagggattc agagatgata
      961 tcggctcctt cccgaatgga gaaagagcaa tcggctacaa acaacgtttt aaaatgccct
     1021 acattggctt gactggaagt tatcgttatg aagattttga gctaggtggc acatttaaat
     1081 acagcggctg ggtggaagca tctgataacg atgagcacta tgacccagga aaaagaatca
     1141 cttatcgcag taaagtcaaa gaccaaaatt actattctgt tgcagtcaat gcaggttatt
     1201 acgtaacacc taacgcaaaa gtttatgttg aaggcgcatg gaatcgggtt acgaataaaa
     1261 aaggtaatac ttcactttat gatcacaatg ataacacttc agactacagc aaaaatggtg
     1321 caggcataga aaactataac ttcatcacta ctgctggtct taagtacacc ttttaacgaa
     1381 gttaaccaga ttttctcccc ggtggtattg tataaccccg gggaatttgt ttacagttac
     1441 aagtaggcaa tatccactaa ttttgtaagc atttaaccta tgtcataacg tgtaatctct
     1501 taccgcaaca caagacctca atatattcaa atcttatctt tattccgata ttcagagaca
     1561 caatgcgaaa aataatcact catttcaaag ttgttttaac gttacttcta ccagtaaccg
     1621 tatctgccca gcagatacag tggcaatcct gtatggccag tcaattcaac cactggtttg
     1681 gtgaggaaaa accgtctcct gacttactat gtggttattt gtctgttcca ttaaaatata
     1741 cagacacagg cggagatgct tcttatgaaa aaaaatcaca agtcaaacta gcgttgacaa
     1801 aattgccggc aaaaagcaag cataaaggaa gtatcctgat aataagtggt ggtcccgggt
     1861 taccaggcat aaatccttat attaactttg actggccagt cacaaatctt cgtgagtcat
     1921 gggatattat tggatttgat cctcgaggcg tcggacagtc cactccgaca ataaactgcc
     1981 ggcaatcaga tacagagact caggaaaaca taaccgaaaa gcaacaagta ttaaataaaa
     2041 ttaatgcctg tatccataat accggagccg aagtcattcg ccatatcggc tctaacgagg
     2101 ctgtatacga tattgatcgt attaggcaag ccttggggga taaacaactg acagccgtgg
     2161 cgtattcgta tggaactcaa attgcagcct tatatgcaga acgttttccc tacaacgtaa
     2221 gatctatcgt tcttgatgga gtcgtcgata tcgatgacct ggaggacaac ttcacatggc
     2281 aactcaaaca ggcacagagt tatcaggaaa cgtttgatcg ctttgcatcc tggtgtgcgc
     2341 gtacaaaaag ttgcccgctt tcttcagaca gagataaggc aataactcag ttccatgagc
     2401 tattatcaaa attacatcac aaacctttat tagacagtaa gggagaaaat atatcttcag
     2461 atgaactcat atcattaaca acagaccttc tgctatggcg ttcatcatgg ccaacccttg
     2521 caactgccat acgccagttc tctcagggga ttgtcagtaa tgaaattgaa actgcgctca
     2581 gtgctccgat agcctcagaa gagtcaagcg atgcttcggg ggtaatcctc tgtgtagatc
     2641 agggggatga gcaattaaca ccagaagagc gaaaatcccg aaaagacgct cttgcgaatg
     2701 ccttcccggc tattaacttt gacaatggac gttccgattc acctgatttt tgtgaattat
     2761 ggccaataca tagcgacctg aacaaaactc gcctgaaaaa tactgttctg ccctctggtt
     2821 tactgtttgt agcacacaaa tacgacccaa caacgccctg gattaatgcc cgtaagatgg
     2881 cagagaaatt ttccagcccg ttactaacaa taaatggtga tgggcataca ttagctctca
     2941 ccggagttaa tttatgtgta gataaagcag ttgtacatta cctgatcact ccacaaaaaa
     3001 tagaaaatat atactgccca ggaaattctg aagcagaaat acaataattt caaaatattc
     3061 cgctatttgc tcccaatgca aaacatatat tgcattggga gcatattcat atttttgtta
     3121 ttattgtatg aaatcgtctc atgtacacgg tccttttttc gccgaaaact gatattactt
     3181 taatcaaaag ttgacgtgcc agattcgaag taaatttata aaatatcaaa tcaatataca
     3241 ttttgcacct tcgagatagt aatgactgtt actgtaacat gagttacaaa cagcttcata
     3301 cccagagcta ctgatgtatc aactcgattc aacaataccc gatatttatt ccatgcctcc
     3361 agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg cagtggcgca
     3421 atatactcac tgaattcctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc
     3481 tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa
     3541 ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata acatggtgaa
     3601 attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct
     3661 ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga
     3721 acaccttctt ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg
     3781 aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa
     3841 cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat
     3901 taaccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg attccatttc
     3961 catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg
     4021 caatgcggcg caggccttaa ctgacgatgg aactctatac ggagccacaa ttcttggcga
     4081 tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat
     4141 cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga
     4201 gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata
     4261 gcatccaacc gcagcacgtt cttgcataag gcgtgtctgc ggtttttcaa ctattcagat
     4321 acatcactcc catcacattc attcctccgc atcataggca tataggctat atcaccttga
     4381 tatttttctt cttcagataa aaactgttat ctatgtatac ttttaaaccc aatccgtgta
     4441 gagtctctgc ataagatagt ttgcagttgc cacttcagct tgcgccataa accgcctgat
     4501 ttttgccgcc acctgttagc attcctgtat acctgaaacg acaatgttta tctacgaact
     4561 ttaagaacac ccaggataaa aattgtcaac tatatcatat ataacacatt actaattcga
     4621 ggctatatga acagcatact gataatcact tctctcctta tcatattcag catttttagt
     4681 catgccctaa taaaattagg gattggcata tccaataacc cagacaaaac cgatgtataa
     4741 gtcaacatat cctgaatcag acatacaata tcgcaatgaa aatcaataat attttaagga
     4801 atatcttcat gaaatcaaaa gacaccctaa agtggttccc tgcgcagctt cctgaagtaa
     4861 gaattatcct aggggatgct gtagtggaag tagcaaaaca gggaagacct atcaatacca
     4921 gaacattgct tgattacatt gaaggaaaca taaagaaaaa atcatggctg gataacaaag
     4981 aattattaca aacagcgata tcagttctta aagacaacca aaatttaaat ggtaaaatgt
     5041 aatataataa acttactttt ttatcatttt tccactttaa caacattttg ctccactttt
     5101 ccacgatcaa acaacttgaa atctggttaa aataacacgc aacactattc ttcttccttg
     5161 agtccgcccg gaactcgaaa aacaaaccga gttaaagcca tttttcacaa aatcgatttt
     5221 gggtctcacc aaaattacgg ggttgcatac gcattcgttt attttcgaac gtgtacatac
     5281 aaatatgcac aaaaataatc aaaattattt tctgagatgc attatgatat gaacaccaat
     5341 ttcgtataga gtctcactat gtctcgaatt tttgcttact gtcggatatc aacgctggat
     5401 cagaccatcg aaaatcaacg ccgggaaatc gaaagtgcag gttttaaaat caaacctcag
     5461 caaataatcg aagaacacat tagcggctca gcagcaacca gtgagcgtcc tggttttaac
     5521 cggttgcttg ctcgcctgaa atgtggtgat caattgattg tgacaaaact ggatcgcctt
     5581 ggttgtaatg caatggatat caggaaaaca gtggaacaac tgaccgaaac aggtatcaga
     5641 gtgcattgct tagcattggg gggcattgac ctgaccagtc caacaggaaa aatgatgatg
     5701 cacgtaattt cagcagtcgc tgaatttgaa cgagaccttt tacttgaacg cactcattcc
     5761 gggatagtaa gagcccgcgg cgcagggaaa cgttttggtc gaccacctgt gttaaatgaa
     5821 gaacagaaac aggtggtatt cgaacgaatt aagtcaggtg taagtataag tgccattgcc
     5881 cgggaattca aaacctcgcg gcaaaccatt ttaagagcca aagcaaaact tcagacacct
     5941 gacatataaa aaataatctc ggtgtgagat gctttacgtc ttccaagccc ccttccttgc
     6001 cgtaaatgga aagatacatc taattataga atttatatgt tttaccctac ggcagtgctg
     6061 gccattcaat atcctgtgca gttgacgtat caacacggtt cagcaacacc cgatactttt
     6121 tccaggcttc cagcaatgag gtttcttcct ccgttgcgat ttccagatct acagcatcct
     6181 gaagtggcgc aatatgctca ctggctacct gcatcaggct gttttttgtt tcttccgcct
     6241 ctcggatccg gaacagtttt tctgcttctg catctttcac ccaggctgtg ccgttccact
     6301 tctgaaactc cccttccggc gataaccagg taacattttc cggtaacgga ccgagttcag
     6361 aaataaataa cgcgtcccct gacgctacgt cataaaccgt tttaccccga tggtcttcaa
     6421 cgagatgcca cgatgcctta tcactgttga aaacagccac aaagccagca ggaatatctg
     6481 gtggtgcaat atcggtactg tttgctggca gacctgtatg aggcggaata tatgcgtcac
     6541 cttcaccaat aaattcatta gttccggcca gcagattata aatttttatg gtccgtggtt
     6601 gttcactcat tctgaatgcc attatgcaag cctcacaata tagttaaatg cgatgttttt
     6661 gacggtgttt tccgcgttac cagcagcgtt aacggtgatg gtgtgtccat gtgaaccaat
     6721 cgcaacggag tgcgtatgag caccaatacc gacagtatgc gcgtgtgcac ctgcagatgc
     6781 tgctgtgccg gacagtgaat ggctatgatt accatctgta ctggtattcg ctaaccaccc
     6841 cgtagacata cctactgagc cttgtacacc ccaggtattt tgacctgagc ttgtataacc
     6901 atattgataa gtatctttaa aaacactggg gttaaatcga cggccatctc tatggctgtg
     6961 attaccagct gcattcgtgc tgccacttaa actatgggta tgcgcaccag tgttattcgt
     7021 ggatttagtg ccgtaatcaa acgacgatgt ggttttcgtc cccaaatccg tactggatgc
     7081 gctggcgctg tgggtatgcg atttaatgcc gtcctgttcc tgagacaata cggcccgacc
     7141 actggcaggt ttgcccttaa tcgtccagcc acgcatatca gggatcacgc ctgacggata
     7201 agcggctgca agtttcgggt atgcagattt gtcaaaagtc tgcccctgca tcagggcata
     7261 gccagacgga acggtatctg atggccacgg gattggtgcg ccaggcggat aaaactgctc
     7321 tgatggcgta tagagtgaat aaactgtacc gtccgttaac ccttccggct tattagcaga
     7381 atatgctggt gacgtatgaa tcgtcacgct ggcattactg gtataatccc attgaatatt
     7441 tacaccagtc gcataatttc cgattgcaac gtaaatatcg taagtatcac cagatgtatt
     7501 gacccaggca aaatttgtaa accctgtcga tgtgcgctgc cataaagcac cagtaatccc
     7561 cttcggatta ccattacctg cacgtaaaac aagttcagat atacctgcct gttgaggtga
     7621 ccccacgtta aatccagcgc caccaatcaa cgtaattgaa acaacagaac tcgcctgtgg
     7681 catggttacc gttgctaatt tgaaccaacc agcaccaccg ctgaatgaca ttgttgttga
     7741 gttaagcgta ccaatatctt tcggcgtcag tgttatatcc gctgaaagcg ccttaccatt
     7801 caccttacgg gcagaaggta cccgaccatt cgcattgtca ttagctgctt tcactgcttt
     7861 cggtgtcgca gcaagcgttt cagatgtgct gttggttgca ctactgagct ggacaattcc
     7921 tttttgtgct gtcgtagcgt cctgtgcggt gtatttcccg ttagccaggt catacgcggc
     7981 cttaacggct tttggcgttg ccgcctgtga ctcggaagtg ctgttagtcg cactgctgag
     8041 ctgaactatc cctttctgtg ctgtcgttgc atcctgtgcg gtgtatttcc cgttagccag
     8101 gtcatacgcg gccttaacgg cttttggcgt tgccgcctgt gactcggaag tgctgttggt
     8161 cgcactgctg agctgtacta tccccttttt cgtcgtgctc gcatcctcaa gcgccacggc
     8221 ggatgcaata tcctctgccc gttttgctgc tgtctcggcg cgcgttgccg cggattccgc
     8281 cgtacttttg ctctgtgctg ccgctgccgc actgccagca gcctctgtcg ccttcgtgga
     8341 tgccgtcgtg gcgctgcccc tcgctgctga cgcctgtctg gtcgcctcat cttttgaagc
     8401 agacgcagat gatgccgatg acgccgccga actggcggac gatgcggcag ccgtttttga
     8461 ggattctgcg ctggtttccg acgctttcgc gttcgtttcg gatgtcttcg ctgcggaagc
     8521 agacctcgct gctgcgctgg cctgttcagt ggcttcgcca gccttcgttg tggctgttga
     8581 agcggatgat gcggcgcttt ctgccgattt tccggcggcg gtggcactga ctgctgcctc
     8641 ctctgcctta atggacgcct cacgggccga tgatgcagct gctgtctcag agtctgccgc
     8701 agctgaggcg ctctgttccg ctgccgtttc agaagacctg gcattcgtct cggacgtttt
     8761 tgccgccttc acggaatttc ctgccgccgt tgccgaggag gctgcactgc tggcgcttgt
     8821 ggaggcgttc gtttctgatg attttgccgc ctctttggag gccgccgcat ctctggctga
     8881 agtggcggct tctgacgctt tcgtcgtcgc agtggatgca gaagtggctg ctgatttttg
     8941 tgacgctgca gcattcgttt ctgacgtttt cgccgcaccg gcactggtgg ccgccgcgct
     9001 ttttgaggac tctgcagcgg cagcactttt tgatgcttca gtggcctttg ttgatgccgt
     9061 tcctgcgctg gaagacgcct cctgagccga agtcgcggcc tgtccggctg acgtgctggc
     9121 tgcacgcgct gagcctgcag catcagtcgc atgggttgcc gcctcacggg cagatgtgcc
     9181 ggcatcactg gctgacttct tcgcggctgc cgtgttctgt gccaccgcgg acgcgttacg
     9241 cgtcacctct tccaccatca gttcaaaacg gcgcagagcc tccggacggg catcatcctc
     9301 cgtcatggca ccgagaaaat cattcagcgt accgggtcgg gaatcttcat acacggtgat
     9361 ggtcccggca tgtgacggcg ggaatccttc caccaacaga ataacgctgt actgaccgta
     9421 ctcaacgtcc atgctgtaac gcccggcttc atccggattt tctgaggcca gcgtgttcac
     9481 caccaccgtg gtgctgttac gttttgcttt cagctggatt gtgcagttct gtaccggttt
     9541 tcctgtgccg tctttcagta cacctgaaat ctttactgcc atattcaccc cacaaaaaag
     9601 cccgcctgaa ccggcgggct gtcataacac tgtgttacct ggctaatcag aacttataac
     9661 cgacacccac gatgaaaccg tcagtgcgcc agtcgccact gccggagcct tcataagcaa
     9721 tatcaatggc cacggattcg gtcgggttaa actgcacgcc agctccccac gccagagacg
     9781 tgttgctgtg gcgaccgtca tcacttccgg tcagcacgtc gtgcgttttc cccttgttgt
     9841 cagttacgcg gagataatcc ccggagaaag tcgacacacg gctgtaagcc acacccgcca
     9901 tcgcatacgc gctgaaccat tcattcacgc gcacagacgg ccccgccatt acgctgaacc
     9961 agcggttacg aacggaatct tcatgccagc gagtatcgct gtaatgggtc agctggcgat
    10021 tcttgtctcc tgcatagctg aatgacgtca ccagccccag cgtatccgta aactcataac
    10081 ggtatttcac gttaatcccg ttcagatcat cactgccggg aacgttggtc cgggcatgaa
    10141 gataccccgc gctcagcgtg gactgatgtt cagacgccca tgcaggcgca ccggatacgg
    10201 ccagacaaat ggctgcggac aaaatggcgg cacaaacttt acgcataatt acctctcgct
    10261 tttctgcaat aaaaaaggcg tcattcctga cgccatttat tggggttata aatatttcaa
    10321 cgaatactga tgccggaagc cgcttttttg gtcacaatca ccgtacagtc ggtgatattg
    10381 cctgccccct gattgccttt ctggaaaatc ttaaactcca gagtgacgct accaccacca
    10441 ctaggcatat caataactgc actgtaacta ccgggaatgg cccctttagt ttctctggat
    10501 gcgattaata cgccgttttt gcgaacttca aaaccataac ccgtgtatcg cgtgcctcct
    10561 gggttatttc cgctccccgg atcgtcatac gctataccgt taaaaataat gggcggaata
    10621 ataatctggc ggtcaaagtt atgatcatcg ctgatggtga ctgtaaccgt accgtttggt
    10681 atttccgtgt taccccacgt accgactttt ttcgggaagg cttttgatac agctttaacg
    10741 aaatcccctc tgacctgggt cgcctccagc atgcccttaa tcgtacagtt ttcatttacc
    10801 gtgacattgt tgagcgttcc tgagttcgca ttcacactac cactgatatc cgcattttta
    10861 gcggtcagct ttccgtctga tgtcagggaa aataccggag gactgccacc gctggtaatg
    10921 gtgggggctg tcaggcgctt caggaacacg tcgttcatga atatctggtt gccctgcgcc
    10981 acaaacatcg gcgtttcatt cccgtttgcc gggtcaataa acgcgatacg attggcggca
    11041 accagaaact ggctcagctt gccttcctcc gtgtcctcca tgctgaggcc aatacccgcg
    11101 acataatgtt tgccgtcttt ggtctgctca attttgacgc cccacatagc attccattta
    11161 tcgttagcgt ccttccactc tttcgaaaac tcatccagtc tgctggcgtt atcctccgtc
    11221 aggtcgactt tttccagcag ctctttaccg agatgggatt cggttatctt gcctttgaaa
    11281 aaatccagat aaccttccgc atcatcgctc gcccgaccga cggcctccac gaatgccgat
    11341 ttgccaacgg tgttcacact gcggatataa aagtaataat catggcccgg tttgatattg
    11401 atactggcgg ctatccagta cagccccgcg ccaaggtagc gggctgtggt ttcaacctgc
    11461 ctgatatcgg taatccgcgt ttccgagaac cagaactcaa actgtaccgt cggatcataa
    11521 accgcaagat gcggcgtggc agttatctga aaatagcccg gcgtcagctc aatccgcgac
    11581 ggtgctaccg gtgcggcaat cctgaacgat accgacgccg gatcgccctg ctgtccccac
    11641 gcatttaccg cccggactgt cagcgtgtac cgtcccagcg ccagttgcgt gaagcggtat
    11701 gtggtttccg ccgtccgggc cgtgctgacc agccgctcac tgccgtcatc cgctgccacg
    11761 gtcaggcgaa gcaggaagct cacgcccttc accaccttcg gcgtgtccca tcgcgccagc
    11821 acctgatatt ccccgctgtc tgcagtgact tcggcggtca gatgctgcac tgctggcggc
    11881 gtgacaccat tcaccgtgcc gctctggtcg ccgtcaaagt gcgccccgtt atccacgatg
    11941 gcctcttttt ccggtacatg ctgcacggcg gtgatggcat acgtgccgtc atcgttctca
    12001 cggatactca cgcagcggaa caggcgctgg cgcagcgtcg gcagcttcag cccccacacg
    12061 ctgtatccgg caacgccgtc aggaacacgg ctcactttta ccttcacacc gtcggtgacg
    12121 gactggacct ccacgctgac cggattgcca cttccgtcaa ccaggcttat cagcgtggta
    12181 ccggaggatg gcagcgtgat ttcacggtcg agcgtcagcg tccgggtctg gctgttcacc
    12241 gccagcacgc gcccgccgat gctgataccc gcatagtcat catcgcagat ttcaatgaca
    12301 tcccccggta catggcgaag cccttccgca cccacgctga agtccacggt ctgcgtttcc
    12361 agcagttccg ttttaatcag ccacagcccg gcgcggtgcg cctgcccccg gctggtacag
    12421 ccaaaggcat ccatcttcgt gacgttacga ccgtaacggg caatggcctg cgtgtcctcc
    12481 acaagctctg tcgccgtctc ccagccgtta tccgggtcaa tccagttcac ctcaacggca
    12541 ttatggcggt ccttcagggc gctgaagctg tagcggaacg gcgcgccatc atccggcatc
    12601 accacattac tgcggttata ggtccacacc ttatctgatg gtcggtcctg cacgaacgtc
    12661 agcgtctgcc cgttccatac cggcatacag cgcatcgccg agcagaaatc actgagaaca
    12721 tcccacgcct tacgctgtgt ggtcagccag gcattacagg tgatgcgcgg ctccgtgccg
    12781 ccaaaaccgt ccggcaccga ctggtcgcaa cactggccga tgacatacag cgcccattta
    12841 tccacatccg ccgcaccaag acgtttcccc atgccgtagc gcgggtgggt cagcatatcc
    12901 cacagacacc aggccatgtt gttgctgtat gctggcttaa acgttccgtc ccagataccg
    12961 ctgtattgcc gcgtctgcgg gttatagttc gacggcacct gcagaatacg cccgcgaaga
    13021 tgataattac ggctcacctg ctggctgccg aactgctccg aatccacctg tacgccgacc
    13081 agtgccgtgt tcgggtagca ctgtttcaca tcgatgattt cggtgtatga cgaccagagc
    13141 gttttgttct gcagctggtc tgtggtgctg tccggcgtca tcctgcgcat ccgtatattg
    13201 aacgggcgcg gcggcaggtt acccaccacc accgaggcca gatactgcga ggtggttttg
    13261 cccttaatgg tgatgtcttt ttccgtcacc cagccaccat tacgctggat ctgaaccagc
    13321 aggcggactt ccgacggatt ccggtccccc tttgaggtgg tttccaccag tgcctgcacg
    13381 ccgaaggtaa agcgcagacg gtcgatgttt gcagacgtaa tggtgcgggt gatcggcgtg
    13441 tcatatttca cttccgtacc cagcaccgtc tcggagccgg aggattcaaa cccctccggc
    13501 ggtgtctgct cctgctcacc ggcacggaac accaccgtga caccggagat gttggtattc
    13561 ccctcagtgt ccagcaccgg cgtactgttc agcagcacgc tttttaatcc atccaccgga
    13621 ccttcaaccg gcccttcgct gatggcatcg atcacactca gcagctgcgt ggacttcagg
    13681 ttgtccttcg cttcgcgcgg ggtatgcccc ttactgctgc ctttacccat tcctcacgct
    13741 ccataaatga caaaaccgcc cgcaggcggt ttcacataaa acattttgca tcagcgacca
    13801 atcaccacaa cctgaccacc gtccccttcg tctgccgtgc tgatctcctg agaaaccacg
    13861 cgagacccca cgcgcatttc cccgtacaga acaggcagaa cattgccctg ggcaaccatg
    13921 ttatccagtg aggagaaata ggtgttctgc ttaccgttat ccgttgtctg tgtacgggga
    13981 gttctggctt tcggtgccag catctgcgcc acaccaccga gcaccatact ggcaccgaga
    14041 gaaaacagga tgccggtcat accaccggtc ccaatggctg ccccccatgc tgcaagggtg
    14101 gctccggcgg taaagaatga tccggcaatg gcggcagccc ccaggacaat ctggaatacg
    14161 ccacctgact tggccccggc gactctggga acaatatgaa tcacagcgcc atcaggcaga
    14221 gtctcatgta actgcgccgt taacccggac gtgctgacgt cccgcccggc aatccgtacc
    14281 tgataccagc cgtcgctcag tttctgacga aacgccggga gctgtgtggc cagtgcccgg
    14341 atggcttcag cccccgtttt cacacgaagg tcgatgcggc gaccaaatcg ttgtaaatcc
    14401 ccgtaaaggc agatgcgcgc catgcccggt gacgccagag ggagtgtgtg cgtcgctgcc
    14461 atttgtcggt gtacctctct cgtttgctca gttgttcagg aatatggtgc agcagctcgc
    14521 cgtcgccgca gtaaattgcg gcgtgattcg gcactgatga accaaaacag cacagcagca
    14581 catcgcccgg ctgtgccgct gacaacggca cctgatacag ccccgtcgcc tccagattat
    14641 ccagatagag attctggccg ttacgccacc agtcatcctc acgataaaag tccggcatct
    14701 caatccccgc cagatgataa gcatcccgga acagtgtgta acagtccgtc acaccgtgct
    14761 caaagcgccg cccggtgaga tgcggcacac agcggaactt atgaatcgtc ccccggcaga
    14821 ccagccacca cggcaaatca ctctgcacct gcagccgccg gtcggcctca ctcagccagg
    14881 gcagaccacc ggggtggctg tggaccagcg ccacaatctc accctgcatt tctgcctgca
    14941 gccagtcttc cggcgacata cggaaatacg cctccggctc accggagata ttcacgcagg
    15001 gaaaatatct ttccccctcc ggcgtgctta ccacgaagcc gcacgactcc gctggcgcac
    15061 atcgccgggc gtgcgccaga atcgctgatt ctgtctgtgt catgggattt actgcgaaag
    15121 tttgttaatg gaaaggaagc cgccaaagtt gccgacgtta ttgcggaact tacaaccgct
    15181 caggcatttg ctgcatttat ccttcgtgat atcggacgtt ggctggtcat attcatccgc
    15241 gacagccgga ccgctataac cgcactcgtc accgcgatag gtccaggtgc aggtgttggc
    15301 cagcatgata cgtcccggaa aaacagcgcc atccgtttcc gtcggcgtgg acagtacaaa
    15361 ggaggcactc accgcgctca gttcgctgca ctgctcaatg cgccagcggc tgatcacctc
    15421 ctgctccgga tcggcataac tgtttccgtt gacgaagttc accgcatcca gaaaacgggc
    15481 gtaaacctta cgccggacca ccgttccgcc gatcagactc tgcatatctt ccgccatccc
    15541 ggtgaccata ccgtacaggt tagaaaccgt cagcgtgggg cgcgtactgg tgcctttgcc
    15601 attcagttca aaaccgctcc cctgaatggg atacggctga tactgtcgcc cctgccaggt
    15661 gaccggctca cctttttcgt tctgctcatt acagaaaaaa taacgttctc caccgacctc
    15721 tgtcaggtcg atttcccaga gcaccacgct ggccgactgc tccgcacggg tgcattcatt
    15781 cagtgtttcc tgccggatat cctgcatcag ttcaccacct gttcaaactc tgcgctgaac
    15841 tcaacacgca gcatactgac ccgcgacgac cattttgcgc aggtcacctt tatctgccgc
    15901 cactcataag gcggcgtcca cagaaaggat ttccagcccc cgtgctcttc cagaaacgac
    15961 tccagtaccg tggcctcctc acgggggaca gaaagcgtca cgctgtacgt tttcaggttg
    16021 gcattcagcc cggcaggcgc tcgctgagaa tagccatcac caaagcgcac ctttcttaca
    16081 gaagggaccg aagccacatc cataccgggt ttcactttcc agcggaaggt cttcatcgtc
    16141 cacctccgga gaacaggcca ccatcacgca tctgtgtctg aatttcatca cgggcaccct
    16201 tgcgggccat gtcatacacc gccttcagag cagcgggacc tatctgcccg ttcgtgccgt
    16261 cgttgttaat caccacatgg ttattctgct caaacgtccc ggacgcctgc gaccggctgt
    16321 ctgccatgct gcccggtgta ccgacataac cgccggtggc atagccgcgc atcagccggt
    16381 aaagattccc cacgccaatc cggctggttg cctccttcgt gaagacaaac tcaccacggt
    16441 gaacaatccc cgctggctca tatttgccgc cggttcccgt aaatcctccg gttgcaaaat
    16501 ggaatttcgc cgcagcggcc tgaatggctg taccgcctga cgcggatgcg ccgccaccaa
    16561 cagccccgcc aatggcgctg ccgatactcc cgacaatccc caccattgcc tgcttaagca
    16621 gaatttctgt catcatggac agcacggaac gggtgaagct gcgccagttc tgctcactgc
    16681 cggtcagcat cgccgccata ttctgtgcaa taccatcaaa ggtctgcgtg gctgcacttt
    16741 ttacctgcga catactgtcc gtggcgctct cttcccactc actccagccg gacttcaggc
    16801 ctgccatcca gttcccgcga agctggtctt cagccgccca ggtctttttc tgctctgaca
    16861 tgacgttatt cagcgccagc ggattatcgc catactgttc cttcaggcgc tgttccgtgg
    16921 cttcccgttc tgcctgccgg tcagtcagcc cccggctttt cgcatcaatg gcggcccgtt
    16981 ttgcccgttg ctgctgtgcg aatttatccg cctgctgcgc cagcgcgttc aggcgctcct
    17041 gatacgtaac cttgtcgcca agtgcagcca gctggcgttt gtactccagc gtctcatctt
    17101 tatgcgccag cagggatttc tcctgtgcag acagctggcg acgttgcgcc gcctcctcca
    17161 gtaccgcgaa ctgactctcc gccttccaca aatcccggcg ctgctggctg attttctcat
    17221 ttgctccggc atgcttctcc agcgtccgga gttctgcctg aagcgtcagc agggcagcat
    17281 gagcactgtc ttcctgacga tcgcccgcag acaccttcac gccggactgt ttcggctttt
    17341 tcagcgtcgc ttcataatcc tttttcgccg ccgccatcag cgtgttgtaa tccgcctgca
    17401 ggattttccc gtctttcagt gccttgttca gttcttcctg acgggcggta tatttctcca
    17461 gcggcgtctg cagccgttcg taagccttct gcgcctcttc ggtatatttc agccgtgatg
    17521 cctcagactc ggcccagtcc tttgctgcca tctctctggc cttttcaaga tcggcctgca
    17581 acgtggcggc tgaaagccca agttgcgcat tcgctctgtc ctcccatgct ccccggagat
    17641 tggcaagaaa tgctgaggtt ttaccgcgcc ggtggctccg gctctgatac cactgccatt
    17701 ttttgtccgc ctcatcaaaa gccttttctg ctttctccag cattccctgg gcagtgtccg
    17761 ggcgaccaat atccagcacc gaatcccaca tggatttgaa tgcccgtgct gtcctgtctg
    17821 cccaggtctc cagcgtgccc atgttctctt tcaggcggcg ggtctggtca tcaaaccctt
    17881 tcgttgcggc ctcgttcgcc gcctgcaatg ccccggcttc atctccggaa cgctgcaact
    17941 gagcaacata cgcaatctgc tccgccgtca cgttatggaa ctgacgtgcc atcgctgtca
    18001 gtcccgacgt cgggtctgtg gtcagcttcc cgaaggcttc agcgaccttg tccacctcca
    18061 cgccggatgc agaggagaaa cgcgccacac tctggctgat cgcctcaaac tgctcaccac
    18121 cacgcacacc ggcattcacc agcgccgtca gtgactcgct ggtctggtta aacgtcagcc
    18181 ctgccgcctg cccggctctg gacaggacca gcatacgatc tgccgtcagt cccgcctgat
    18241 tgccggaaag gaccagcgtt ttgttgaaat cggacagggt tgagttgccc tgataccagg
    18301 catacgccag cgcaccggtc gccaccgcca gcgaggtggc ccccaccatc ggcagggtga
    18361 tcgcaccggc aagccccctg aacatgggga tcatcccgcc gaaggagtcc ttaacctgac
    18421 ccccctgttg cagcaggatc agccacgggc tttgcccgcc tgcaagctgc gtggccacgt
    18481 cggtgaactg tgcaggcagc atacgcatgg cggctttata ctgcccgacg gaaatccccg
    18541 ctttctgtgc agccagcgcc tgtcggctca gcgactgttc aacgactgcc gctgtttttt
    18601 tcgcatcagt ttccgtaccg gaaaaatgac gcctgactct ggccatctgc tcgtcaaatc
    18661 tggccgcatc cagactcaaa tcaacgacca gatcgcctac cggttcagcc ataccggact
    18721 cctcctgcga tcccttctga tactgtcatc agcattacgt catcctccgt catgtccgcc
    18781 acatccgggg aagcggggat aacttcattc ccgtccgggc caaagcggac acctccggca
    18841 agccctgccg ctttctgcat cagcacatca tcttcaggct cttcgtcagc ctcgcgccgg
    18901 ttcagcagac tgaaatccag cggatgcata tccggatcgc tgaaaaacag gctgagcacg
    18961 gtgtacgtca gcccggaaaa gtgcatatcc agcagaacat catgaaaata atgggtactg
    19021 taaaagcggt gccagtcggc atactccgtg gatgacatcc cggcaagcat ggcacgccag
    19081 tcgggtcgcc ccatctcacg cgccagtttc agggcaaaac tcagctcacc atcgaacact
    19141 ttcccgcaga aacaggctct gcaggcccgg cgtcctctgt ctgttcagga gcatcattca
    19201 ccacaaactc atacataccg gacagccggt acaccacgtt ttcagcatga gaaattgcct
    19261 ccgtgggcca ggtggtaagc acttcctgct caatctgttt aacggcttca ttcatggaag
    19321 gcagctttgt cttcttcgga tggttatgcc acagggacat cgccaccaga aacgcgccgg
    19381 ttctgatggc gtcttccaca gtaaacttcc ggttgctgtc tgactccgcc tgttctgcct
    19441 gccgtttcat cagggcgaga tgctcaattc gctgcagggc tgacagttca gaaagcgtga
    19501 cggtcacacc gttatgttca aatgattcgg ttttcaggaa catcgctgac tccccggatt
    19561 aactggcggt gacggtgatt tctgcaaccg cagcaagttc accattaccg gatacgaccg
    19621 gaatgttgac cttgcctgca gcaacacctt tcacggtgat ggtcatacca ctgaccgaca
    19681 cggtggcttt tgttttatcc gcagacaccg cacggaagct cttgtcggtt gcgccttccg
    19741 gctggaatgc cacggtcagc gtggtgctct gccctttcac caccgaggtg ctggcaggcg
    19801 tcacagtcat gccggttgtc gccgtcaccg tgctgcgatc ttctgccatc gacggacggc
    19861 ccacgttggt gactttcacc gtgcgggtga tcacttcctt cgccgtcacc gccttaccga
    19921 tactgctgac ccagccgcgg aacacatcga ccgtgccgtt cgggaagcgg attttatagg
    19981 ctcgggtatc cccttcatta aaccacgcca gcagcgcctg ctgcccctgc tctccgggca
    20041 tccacgccag cgtgaagctg gtatctccgg cagatttctg cccctgcccg gtcgcagtcc
    20101 agtctgcatc ttcatcatcg agatagctgt cgtcatagga ctcagcggtc agttcgccgg
    20161 gcgtcaggtc tttaactttt gccagacgcg accagtcaac gtctgaaagc ggattcgcat
    20221 aagggtcacc gcttccctta taaacccaca gggtggtccc ggcacctttc accggcatta
    20281 caggatttgg tacaggcata gcgtcctcac atttcatagg taatgacata agtcagatcg
    20341 gctgaactcc acaggcccgc atcatcgtcg cgccggtagt catagccgct ggccaccata
    20401 ctggtgatca aatctgacag tgccgggata tcactcatca ccggataaat ccgggactcc
    20461 atccacgcat ccagctctga atccggcacc tgagcaggca ggaaaacttc aatatgcagc
    20521 tccgcctgcc atgtatcgct gtccagctct tcgcccgtgt attcagcgcc ggtgagataa
    20581 acggcaactg ccggaaaatc cgcctcatca aaaacagcgg ggcgaccatc aaaaaacgtc
    20641 gccccggtgt catgcttctc cagtgcatcc agtacggctg cacggagttc agtatgtttc
    20701 atcgctttat taccattctc agttgatgct gcagcgcata gcccagctct ttcggaagac
    20761 gttcacgccg tatccgttca atattctgtt taaacgccgt ggtcagcggc accgccatcg
    20821 ggattttcac cacatcaatg gggtaacggt ttttcccggc cacacgctgc atgacatgcc
    20881 accggccatt tttcagttgc tgaataaacg cgccgggaat acgacggttt cccaccacaa
    20941 gcacgctgcc gccacctttc agggatgaac gctgcccctt tttacgacgt ctgcggcggg
    21001 aaaggacaac ccgcgcgtta cccagcttga ttacgggcaa atccccccgg ttaaccctga
    21061 ttctggcctg cggatttttg accgtggccc ttttcagcct ggccctttcc tttaccagtt
    21121 tccggcgtac ctttgtctca cgggcaacct gtgacgcaga ctgcgatatc gcggatgacg
    21181 caacgcggtt aatggccatt gcggcggcac caggcaccgc cgttttgctg atacggctga
    21241 ggttttcaac ggcctgctca agacctttta tggccataca tccccctttc agcggcgacg
    21301 gttaacggca ggcggcacgc cacgcccaag ccagagatga cagcttccgc catcatccgg
    21361 cgaaatccgg tccacccaga agttttcctc gccgatggtc agtgtgtctc cacgccgcag
    21421 ctgccgcaca tcatcagtcc ggacaaacag ggacgggctg gagccttcaa cgcgcacccc
    21481 ctgtccggca tagctgatat tttcagggtc atcaaaaaca ccacgtatca cagcaccgga
    21541 cagctcaccg gatgtcatgg tggctgacgt tcccatgtac ccgcgtatcg tttcatcggc
    21601 gcgggcaatg gcagcatcga acaggttatc ggaatcagcc acagcgcctc ccgttattgc
    21661 attctggcca ggccatgttc tgtcatttcg gctgccacac cggcagagac acgaaacgcc
    21721 gttcccggca gcacaaatgc cacaggctca tcccgcgtgg cgtgaagtgc atcagtatgc
    21781 agcgtcacca gtgccacgac cgtgaccaga gcagccgtat caatcacggt atccggctgc
    21841 gctgatacca cctcattttc atgtccggtc agcgcatttt ccgggctgac agatgtgtcc
    21901 tgaccggcag cgtcatccgt gtcatcaagc tcctcttcca gctctgccac acggagcacc
    21961 agttcttctt tcgtccccgt caggctgaca tcacggttca gttgctcacc cagcacctga
    22021 agacgggcaa tcagttcatc tttcgtcatg gactcctcca cagagagaaa atggccccga
    22081 agggccatga ttacgccagt tgtacggaca cgaacgcatc aggatcagcc agcagcatca
    22141 gcggtgctga ctgaatcatg gtgaactcac gcgccggatc gccggtggtc acccagtttt
    22201 tcgggtagcg ggcagaggcg ttaatacctt cgcgctgtgc gtccgcatcc tgaatgcagc
    22261 cataggtgcg cagaccgcgt gcctgagtgt tccccagcac catcgtgttg tccggcagga
    22321 agttcttttt gacgtcgttt tccacgtact gtccggaata cacgacgatc gccgtatcgc
    22381 catacatccc cttataggac accgcttcgc ccaggtcttt taccgctgtc tccagctcgg
    22441 aatgagagcc gcgacgggta tccagcttct ccttgacggc tttgaaggaa cggaacagcg
    22501 cccagccttt cggatcaaac acgatgatat tcaccacgcc gctggcgttc agcgcatagg
    22561 cttcgatatc gtcggtcggg tcatacgtgg acttgtcacg cttgctccac tccgtaccac
    22621 cggactgtgt gatgttgttc gccgcactgc ggcccatatc cacctcaacc ggatcgaagg
    22681 cttcaccggt catggtgtat ttgcccttaa gcacagcaga aactgcctgc atctcttcga
    22741 cctgagcaat ggccagctct tcgtctcgca tgttctgcat gatgatgcga cggcggcggt
    22801 aagccgggtc cgccagattc tgcggatctt catccggcag gcgacgcagg gtcatctgcg
    22861 gattcacctc atgcttgggt ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac
    22921 gggaacggat aacctcaccg gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc
    22981 ccggaatttg tgagagatag actttctccg tggtgaaggg atagctctca cggaaaaaga
    23041 gacgcagaaa cagcggatca aacttaaatt tctgctcatt tgccgccagc agctgggcgg
    23101 ttgtgtacat cgacataaaa aaatcccgta aaaaaagccg cacaggcggc ctttagtgat
    23161 gaagggtaag gttaaacgat gctgattgcc gttccggcaa acgcggtccg ttttttcgtc
    23221 tcgtcgctgg cagcctccgg ccagagcaca tcctcataac ggaacgtgcc ggacttgtag
    23281 aacgtcagcg tggtgctggt ctggtcagca tcaaccgcca gaatgccaac ggcagtaccg
    23341 tcggtggtgc catcccacgc aaccagctta cgggtggagg tgtccagcat cagcggggtc
    23401 attgcaggcg ctttcgcact caatccgccg ggcgcggttg ccgtatgtgc cgggtcactg
    23461 ttgcccagcg gctggtaatg ggtaaaggtt tctttgctcg tcataaacat cccttacact
    23521 ggtgtgttca gcaaatcgtt aacggcatca gatgccgggt tacctgcagc cagtggtgcc
    23581 ggtgccccct gcatcagacg atccagcgca gtgtcactgc gcgcctgtgc actctgtggt
    23641 gcagctgcca gaatgcggcg ggccgtttcc acggtcatac cgggggtttc ggccagcaca
    23701 cgtgcctgtt cttcgcgtcc gtgagcttcc tcacagttga ggatccccat aatgcggctg
    23761 ttttctgccg caaccgcagc ggtgatctgc gcgttcacgt ccggctgcgc cgcgctggcg
    23821 ttttcgccct ccgtcgcttg caccacgtca gtaacgtcag cctgcgaagc agtggctgaa
    23881 acagttgttg attgagtctc tttggtcatt cgccctcctg agagacggga tttacgtgca
    23941 tccagtgcat cacgcataac ggtgatcgca tcggtgctgt tgacaagttc atcagccagt
    24001 ccggcatcaa tggcctcctg accgctgtac actgcagcct cggtatccag cacagcctgc
    24061 acggacaggc cggtatatgc cgacaccttc tgcgcaaaca tccggcgggt tgcatccatc
    24121 cgggactgca gtgtctcccg gacatcaccc ggtagatggc tgtaggggtt gccatccacc
    24181 ttatggctgc cgctgtaaat cagcgtgatt tccacgccct gtttctccag cgcagcgccg
    24241 taattactgt gagccatcat gacgccgatg gagcctgtcc gggcggtctg cgtgaccaga
    24301 cgccgggagg cggcgctggc aagcagctga cctgcactgc agttcatgtc gttggccagc
    24361 gcccataccg gctttatgtc acgcacacgg gcgatgatgt cagcacagtc aaatgctccc
    24421 gccaccatcc cgcccggtgt gtccatatcg agcagaatgc cgtccaccat cggatcgctg
    24481 gcagcctgtt gcagacgggc gataatgccg ttgtaaccgg tcattcccga atacggctgc
    24541 agcgcccgcg tccggctgac cagcgtaccg gacaccggca gcacggcgat gccgttcatg
    24601 acctgataac tgcgggcctg tcgtggtccg tcatcatcac cggataacgc cagcgccgcg
    24661 ggtgcctctc cggcagtcag gctgtcgccg gatactgcat ccgtcaggcg actgatccca
    24721 agctggcctg caagcgcaca aaagaaaacc cgcgcatagg cgggttcaag catcagcggc
    24781 tcattaaaag ccatgctggc aatatgcggg agattacgca gctctgctgt cactcttctc
    24841 ctcctctgtt gattgtcgca gtccggattc aaatgccgca gccgcccagg cgggcggttt
    24901 aagaccggct gcacggcgct ccatcgtttc acggacctgc tgggcaaaaa tttcctgata
    24961 gtcgtcaccg cgttttgcgc actctttctc gtaggtactc agtccggctt ctatcagcat
    25021 caccgcttcc tgtacttctt tcagaccatc gatggccata cgaccggagc ctatccagtc
    25081 acagttcccc caggcgctgc gggcttcctg aaagctgaaa cgcgcttttg aaggtaacgt
    25141 caccacgcgg cgaacgatgg cctcttccag ccagcacaga aacatctggc tcgcctgacg
    25201 ggatgcgacg aattttcgcc gccccataaa gtacgcccac gactcgttcg cactggcccg
    25261 tgccgtggag tagctcatct gggcgtaatt ccgggaaagc tgctcatacg agacacccag
    25321 cccggcagcg atataccgca acagtgactg ttcaaacacg gagtagccgt tatccgtgtc
    25381 ctgagccgtc tgcaggttca gtgagtcccc cggcatcagg tgcggcactt ttgcgcctcc
    25441 cagacggacc ggtgctgcgg cgtaatacgc ggcaatttca ccaatccagc cggtcagcct
    25501 ttcccgctgc tcctgactgt tcgcgcccag aataaaatcc atcgctgact gcgtatccag
    25561 ctcactctca atggtggcgg catacatcgc cttcacaatg gcgctctgca gctgcgtgtt
    25621 ctgcagcgtg tcgagcatct tcatctgctc catcacgctg taaaacacat ttgcaccgcg
    25681 agtctgcccg tcctccacgg gttcaaaaac gtgaatgaac gaggcgcgcc cgccgggtaa
    25741 ctcacggggt atccatgtcc atttctgcgg catccagcca ggatacccgt cctcgctgac
    25801 gtaatatccc agcgccgcac cgctgtcatt aatctgcaca ccggcacggc agttccggct
    25861 gtcgccggta ttgttcgggt tgctgatgcg cttcgggctg atcatccgga actgtgtccg
    25921 gaaaagccgc gacgaactgg tatcccaggt ggcctgaacg aacagttcac cgttaaaggc
    25981 gtgcatggcc acaccttccc gaatcatcat ggtaaacgtg cgttttcgct caacgtcaat
    26041 gcagcagcag tcatcctcgg caaactcttt ccatgccgct tcaacctcgc gggaaaaggc
    26101 acgggcttct tcctccccga tgcccagata gcgccagctt gggcgatgac tgagccggaa
    26161 aaaagacccg acgatatgat cctgatgcag ctggatggcg ttggcggcat agccgttatt
    26221 gcgtaccaga tcgtctgcgc gggcattgcc acgggtaaag ttgggcaaca gggctgcatc
    26281 cacactttca ctcggtgggt tccacgaccg caactgccct ccaaatccgc tgccaccgcc
    26341 gtgataaccg gcatattcgc gcagcgatgt catgccgtcc ggccccagaa gggtgggaat
    26401 ggtgggcgtt ttcatacata aaatcctgca ggtcccctgc gtcgctgtgt catgccggtc
    26461 tgcacttcca gctctgcaat atattttttc aggtcagaca cggaagtggc cgtaaactcc
    26521 acccttcgtc cgtctttctg tactgttgcc acccgtttac ctgtcatcag gtcatgcagt
    26581 gccgcacggg cagcggcaag ttcttcctgt cgcgtcattc atcctctccg gataaggcac
    26641 gggcgtaatc tgccagtgtt ttcttgttgg ttgctgcacc atcctcttcc tgcaggctcg
    26701 ccagcagcgc actgagatcc agctgccagc gggaaatact gatgcgcagc gccgccagcg
    26761 cataaacgaa gcagtcgagt gcctcattgc gtcgcttttt gctgtcccac agtatttttt
    26821 tcctgccatc cacccatttt tcgacctgct cttcagcagt cagctgctgc gcttcggtca
    26881 gatcaaaaat atccgggtta ttcgggaagt gaacggcacc gggaagcggt tcatcccctt
    26941 ccggcgtcag tgtgaagcgg ttataaatct gctctttcgc ggtatccgta ccgatttcgg
    27001 taaggtaaac cccgtttttg tttcgcttac gtggcatgct ggccaccggc tttccgtaga
    27061 cggatgcccc tttaatgggg atcacccgga acagcccatg ttttttcgag cgttcataca
    27121 caatggtcgg gtcaatcccg ccagtatccc agcagatacg ggatatcgac atttctgcac
    27181 cattccggcg ggtataggtt ttattgatgg cctcatccac acgcagcagc gtctgttcat
    27241 cgtcgtggcg gcccataata atctgccggt caatcagcca gctttcctca cccggccccc
    27301 atccccatac gcgcatttcg tagcggtcca gctgggagtc gataccggcg gtcaggtaag
    27361 ccacacggtc aggaacgggc gctgaataat gctctttccg ctctgccatc acttcagcat
    27421 ccggacgttc gccaattttc gcctcccacg tctcaccgag cgtggtgttt acgaaggttt
    27481 tacgttttcc cgtatcccct ttcgttttca tccagtcttt gacaatctgc acccaggtgg
    27541 tgaacgggct gtacgctgtc cagatgtgaa aggtcacact gtcaggtggc tcaatctctt
    27601 caccggatga cgaaaaccag agaatgccat cacgggtcca gatcccggtc ttttcgcaga
    27661 tataacgggc atcagtaaag tccagctcct gctggcggat gacgcaggca ttatgctcgc
    27721 agagataaaa cacgctggag gggtcatccg gcgtccattt gaggccaaac ggcgtctctt
    27781 tgtcgccaaa tttaagatac tgctcctccc cgcaatgcgg gcaggcaaca tgaaaacgca
    27841 taaaatgcgg ggattcactg gctgcacgct caatctgaca ggtgcctctc acttttggcg
    27901 tggagccacg gatggacttt ggccagaccg agccttcaat acgcttgtca cccaggaacg
    27961 tcggagagcc ttcctgttca atatcatcat caaaagcagc aagttcatca taacccgcca
    28021 catccaccga cttttcacgg tagttttttg ccgctttacc gcccaggcac cagaagccac
    28081 gcccattagt gaaacgcttc atggtgagcg tgttatcccg gtgctttttg ccataccacg
    28141 gggccagcgc cagcagcgac ggaatatcac gaatagtcgg ctcaacgtgg gttttcataa
    28201 agttctcggc atcaccatcc gtcggcaacc agataagggt gttgcgctgc ttatgctcta
    28261 taaagtaggc ataaacaccc agcagcattt tggaataacc gacacgggca gacttcacca
    28321 cattcacctc acggatgtag tcgctgccca tcgcattcat gatggcccgc tgaaagggca
    28381 gtgtttccca gcgcccttcc tggtatgcgg attctttcgg gagatagtaa ttagcatccg
    28441 cccattcaac ggcggtctgt ggctccggcc tgaacagtga gcgaagcccg gcgcggacaa
    28501 aatgccgcag cctgttaacc tgactgttcg atatattcac tcagcaaccc cggtatcagt
    28561 tcatccagcg cggctgcttt gttcatggct ttgatgatat cccgtttcag gaaatcaaca
    28621 tgtcggtttt ccagttccgg aaaacgccgc tgcaccgaca gggggagccc gtcgagaata
    28681 ctggcaattt cacctgcgat ccgcgacaac acgaaagtac agaatgcggt ttccaccact
    28741 tcagcggagt ctctggcatt cttcagttcc tgtgcgtcgg cctgcgcacg cgtaagtcga
    28801 tggcgttcgt actcaatagt tcctggctgg agatctgcct cgctggcctg ccgcagttct
    28861 tcaacctccc ggcgcagctt ttcgttctca atttcagcat ccctttcggc ataccatttt
    28921 atgacggcgg cagagtcata aagcacctca ttacccttgc caccgcctcg cagaacgggc
    28981 attccctgtt cctgccagtt ctgaatggta cggatactcg caccgaaaat gtcagccagc
    29041 tgctttttgt tgacttccat tgttcattcc acggccaaaa acagagaaag gaaacgacaa
    29101 aggcccaaaa gttcgttttc agcacctgtc gtttcctttc ttttcagggg gtattttaaa
    29161 taaaaacatt aagttacgac gaagaagaac ggaaacgcct taaaccggaa aattttcata
    29221 aatagcgaaa acccgcgagg tcgccgcccc gtaacctgtc ggatcgccgg aaaggacccg
    29281 caaaatgata ataattatca tctatatgtc acaacgtgca tctacgccat caaaccacgt
    29341 caaataatca attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa
    29401 acaacttcag acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtcaacgaag
    29461 aacaaaaccc gcagaacaac aacccgcaac atccgctttc ctaaccaaat gattgaacaa
    29521 attaacatcg ctcttgagca aaaagggtct gggaatttct cagcctgggt cattgaagcc
    29581 tgccgtcgga gactaacgtc agaaaagaga gcatatacat caatccaaag tgatgatgaa
    29641 taaacatccc ggtttcttcc accatcgcac cggaaaagcg actatgaggg taaccctgcg
    29701 tctgtcagca cagtaaaacc cggtgtgcat cgtttttgat tattcccgca cactcacgca
    29761 gaaggaattc cccgtcgggc tacggtcatg gttaatgcgg gaatacggcg acgatacagc
    29821 gcagctaaaa gggtaatgga cagatagagc ggtttatttc attccacagg attctgagtg
    29881 cccccctcct ccaataggct gagcatccac ctatatagtt ttaattttca tcaatccatt
    29941 taactatcgt ttaattgttg tcacatagga ttctgccgtt tttaacaatg caggataata
    30001 agatgaaaaa aatgttgttt tctgccgctc tggcaatgct tattacagga tgtgctcaac
    30061 agacgtttac tgttggaaac aaaccgacag cagtaacacc aaaggaaacc atcacccatc
    30121 acttcttcgt ttcgggaatt ggacaggaga aaactgttga tgcagccaaa atttgtggcg
    30181 gcgcagaaaa tgttgttaaa acagaaaccc agcaaacatt cgtaaatgga ttgctcggtt
    30241 ttattacttt aggcatttat actccgctgg aagcgcgtgt gtattgctca caataattgc
    30301 atgagttgcc catcgatatg ggcagctcta tctgcactgc tcattaatat acttctgggt
    30361 tccttccagt tgtttttgca tagtgatcag cctctctctg agggtgaaat aatcccgttc
    30421 agcggtgtct gccagtcggg gggaggctgc attatccacg ccggaggcgg tggtggcttc
    30481 acgcactgac tgacagactg ctttgatgtg caaccgacga cgaccagcgg caacatcatc
    30541 acgcagagca tcattttcag ctttcgcatc agctaactcc ttcgtgtatt ttgcatcgag
    30601 cgcagcaaca tcacgctgac gcatctgcat gtcagtaatt gccgcgttcg ccagcttcag
    30661 ttctctggca tttttgtcgc gctgggcttt gtaggtaatg gcgttatcac ggtaatgatt
    30721 aacagcccat gacaggcaga cgatgatgca gataaccaga gcggagataa tcgcggttac
    30781 tctgttcatt gctgacccca caaacagatt tcacgctcaa tctcacgacg agtcatgaga
    30841 cctttccatt gcttaccgcc agcatatgtc cagcgacgta gctgatcaca tgcgcctttg
    30901 atatcgccct ggtttatttt gcgaagaagc gtcgatgttc tgaaattgcc agcacccacg
    30961 ttgtaaacga atgagtaaag agcgccgcgc gttgtttccg gtatatcgac tttgatgtac
    31021 gggttaattt gtctggcgac agtggcaagg tctttattca agagtgcttt gcattctgct
    31081 ttggtatacg ttttaccgag catgatgtct tttcctgtat gcccgtgaca tacagtccat
    31141 acaccaacaa tatctttgta tggtatgtag ctgacacctt ccagaccatc gttaccactt
    31201 gggccagtga ttaacactga tgctatagca attgctccgc caccaatagc agcagcaacg
    31261 gcttttcgta atgatggagg cattattcac ctctcgcagc cttgcgctta tcttctttaa
    31321 tcttgaaata aaggtttgtc aggtacgtca gcaggccaaa taccaggcta cccagcacac
    31381 ctattgctgc ccactgtgag ggcgtgactt tatcgagcag ctgtaaaaac cagtaaccgg
    31441 cactacctgc tgaggtgcca taggcgacac ccgttgttaa cttatccatg gatttcataa
    31501 ccccacctcg cagacaaagc gggtgtaaat tgagggaata caacgtatcg caaaaaagca
    31561 gaaacgtaac agactcggag tcagtgaata actcaggtat tgagttatca gctaatatcg
    31621 agactcaaaa aatggaaaaa ccagctcgac ggcgggttta agctgtgtga cgaagtaacc
    31681 actcttaaca gcataaccaa ttttttacgt acgtaaacca ctgaatgata tttatgagaa
    31741 tgctaccgag tgttcaaaac accaccacaa atacataaga aaatctcaac aaataaccaa
    31801 caaataattt ccagcgttat ttttagccaa tttaaattga accttcaaat catagagcac
    31861 ttataaataa caaccgttaa tataaattgg ctaatagatt tatttttatt cagccaagag
    31921 ccatgaatag gattcgatag aaaaaagttc agataaaaat agagatctac ttcacaaatc
    31981 aaacgagaaa ccaaaactta catcttgaaa taatcatatt gattagatga atatttatcg
    32041 cgcagtgaca tcatttttta ataatagttc aaaaaaaagg gctcacgatg aaaaaattaa
    32101 cagtggcaat ttctgctgta gctgcatcag tactgatggc gatgtctgct caggcagctg
    32161 aaatttataa taaagacagt aacaagctgg atctgtacgg gaaagttaat gctaagcact
    32221 acttctcctc taatgatgca gatgatggtg atactactta tgcccgtctt ggcttcaaag
    32281 gtgaaaccca aatcaacgat caactgactg gtttcggtca gtgggaatat gaattcaaag
    32341 gcaaccgcgc tgaatctcaa ggttcctcca aagataaaac ccgtcttgcc ttcgctggcc
    32401 tgaaattcgg tgactacggc tccatcgatt acggccgtaa ctacggtgta gcatacgaca
    32461 tcggtgcgtg gactgacgtt ctgccagaat tcggtggcga tacctggacc caaacagatg
    32521 tgttcatgac tggtcgcacc actggtgttg caacctatcg taacaacgac ttctttggcc
    32581 tggttgatgg tctgaacttt gctgctcagt accaaggcaa aaacgatcgt agcgatttcg
    32641 ataactacac cgaaggtaac ggtgatggct tcggtttctc tgctacctat gaatacgaag
    32701 gattcggtat cggtgcaact tatgcgaaat ctgatcgtac cgacactcaa gttaatgcag
    32761 ggaaagttct tcctgaagta tttgcttccg gtaaaaatgc agaagtttgg gccgcaggtc
    32821 tgaaatatga cgctaacaac atttacctgg ccactaccta ttctgaaacc cagaatatga
    32881 ctgtatttgc tgatcacttc gttgctaata aagctcaaaa cttcgaagct gttgcacaat
    32941 atcagttcga tttcggtctg cgtccgtccg ttgcttacct gcaatctaaa ggtaaggatc
    33001 ttggagtatg gggcgatcag gacttagtca aatatgttga tgtaggtgcc acctattact
    33061 tcaacaaaaa tatgtcaact ttcgttgatt acaaaatcaa cctgcttgac aaaaatgact
    33121 tcactaaagc actcggtgta agcactgatg acatcgttgc tgtaggtctg gtttaccagt
    33181 tctaatctga ttacgaaaaa gatatgttgc gggaggcttt gcctccccaa catataagtg
    33241 gctccctcaa gccacttcct ttaggagcac aaccttgctt ctaactatat aaaccttctg
    33301 ttatatatta ccctttattt ttgggggcgt ctcgacgccc catttttaat aatttttagt
    33361 aaacaattgg catattaatt agagttatta acaacgatat ccatctctaa ccggatatct
    33421 aatgccatta acatcccttc aattataccc tcagccttct gtaacctttt cccgatataa
    33481 ccatcagagc agcaatgctt acctgccagt gacatgaatg tcataccgac tacataataa
    33541 tctactaata aatcgtgcaa atcgctgttg ttctttttca gacgggccat gcacccgcaa
    33601 atgatcatcg cgtcatcgtc acaacattgc gggcgagatt ttacttttga aggaattaat
    33661 cccttaaaac cggcagcaat ggatgaccag gttacatcct cgtggttatt agccgcccat
    33721 gccccccaac gctcaagaac catctgaata tcacgcatca actttcccca caaaaatcag
    33781 gctagcacgc caattgccag caaaaatcaa taaaacgata taagcgcagg cgcgcccagc
    33841 ggcggcgaag acgttctgcc atataagact caaacatcat tcatctccca gttcagtgat
    33901 ggtcagttcc agcttcccac ctttggtaac aggcatcttc acaacgcggt aatcaacgac
    33961 ctgaacatca tccagccaga aacctgcttt ggtaagtgcg tcaaaagcag ctttttgcag
    34021 attatccagg tcacggcgac ggcgatccgg catgtgacac tcaatgcgga ttttcacagg
    34081 aatagccagg ccgatatcca gcattgcgtt tttaatgatt cgggcgacgt tatcgcggta
    34141 tgcctgcccc tctgcgctga tgtgcgtgcg cccgcgatta tggcggtaat agcgattatt
    34201 gctcggaggc cagggtaatg tgatgtggta agtattcacg ccttgattac cccctctttc
    34261 agccagataa cctgcgttct cgccatacct tccagcgcgc attcttttgc gtactcagca
    34321 tcgacaaaat gcgtgcggcg atcgatttcg tcgtggcagg cagaacatgc aatggtggca
    34381 atcaggtctg gcggtttaat accggtgccg cacaatccag ccagccggat atatgccagt
    34441 acagacgttt cagggttgcc attacatacg ccagggattc ttacctggca ttcccgacca
    34501 cgcgctgctt ttctcaaatc agccatgatt cctccttgct gccagtcgca accatttttt
    34561 atcaaccagg ctggcggtat atccgagcag tgttggtatt tcggatggct tcagctcagg
    34621 tttacgctta cgacgatttg gtactttgta gatgtgtccg ttcatgacac gaataagcgg
    34681 tgtagccatt acgcctcctg cttgtcgcgc agcagctgga actcgcagct ctgcggaata
    34741 gtcaggtgac agccaatatt catcgcccag gcttcaacct tacacaggaa gacatacatc
    34801 tctccggtat caagatcgga ggtatggcgt aacgactgga tagtagtgat ttcgccggtt
    34861 acgacatcaa ccaggtcctt ggtttcataa ccgaggtatg tgtgtttgag agcatctttt
    34921 acccatgctg cggtagcgaa cgatttcccc ctgctgatga ggtattcact gatttcgctg
    34981 taccacatgt ggctgagtgc attctgggaa agactgcgtc tctctcgcca cggtttaagc
    35041 accatgcgaa agcatttgcc ttcctccaga taaggctgga tctgctggcc gatagcggtg
    35101 aagttgccgc gatgtaattt gatgccgtct tgtgagaggt tcacgcttca cctccgcaga
    35161 ggtcaaacgt tagatgcaaa gaattgcagg tgcatttctg catctgtgac gggagaagtg
    35221 agtttggatt gtgtgtgcgc ataaacgtcc ccgtttagcg cagaagtcac cggagttgtt
    35281 caggctccga tgagatgatt atggcgggtt gattattgaa aatcaatttt gtggatttag
    35341 gcgcaatatg aaggatcatt agcagctcta ataccgttct gaatcgattg ctcgcaccct
    35401 attgtgagcc aggaaatatt tttgctcgat gtaagtcaag ccaccggctt cacgtcgctc
    35461 agatacttga ggaagtggag tgacattctg gcaggcggtt agcatcacct gctcgccata
    35521 accaagcgag tgtacaaagt tcgctgtcgg tgaatcacct ttttattaga gcgcgctgca
    35581 cttctcattg aggataattg gtacccagta gacgtatttt ttcgctactg agtacctgtg
    35641 acgatggcaa tcagatcggc actacgacaa gtgaaaaaaa tcacattgta ttaagcatga
    35701 tttcattaaa aactggccaa tagttattaa aagacccctt gcggggtctt ttaatttatt
    35761 gttttttaag catttccttg atttttttct tactttcttc agaaattctg ctttggttgt
    35821 tgatattttt ctgcttcata agccccttca ctctcagtta atcactgttc aattattaag
    35881 ctggtttgct tatactttct tcgccagtgc gtaatccatc aaatgcctca tcattgtctg
    35941 ctgatttgtg agttatggct ttctttttat ctgtaggtat ttctctcaca tcaggactga
    36001 gactttgagt ctcatcctca gcaggaacaa cctctggtaa ctccgtttct tcttcgaatg
    36061 aaacgttata accacgctct atagctacta gcttgacctt ttcataaaac tctgtagaaa
    36121 tcaagtcaaa aatcggcatt gattgcgtca atattgtaac tatctcagtt tcatctaatg
    36181 ctgatagcac ttcttcagca atgtcccatt tttctaaaag tatagcagcc cccagagcaa
    36241 gccaagcttc gtttttatct atcacttcat tcagcacttc ctcaagctct tgttgctgtt
    36301 ttgctgaaag cggctgcttt tgtaatttgt tgcaaagaac tctatttata agcaataact
    36361 gcttatgttt agacggtatt gttcgggatt caagtatgtc cataaggcta ttcgcaattt
    36421 tgaatttttt agcatccatt aagtccacta atccatttac aagcttgcca acttcaccat
    36481 cattcatttt gttataactt gttattcttt ctattgactt ttgtatttca gaatcattat
    36541 ccaaatgaat ataggaacga agtatcaata cttctgtttc gagagatttc ccattagcca
    36601 gatgtttagt agcatattct acacattcat tatactctcc agcacgaaat gcagagtttg
    36661 caatcaaata attgggggta ttataaaaat gaatcccctc aggagaacca gcaaatttag
    36721 tttcctttaa cattatttct aaagatttag tgatcgaatt atcatctaac gatgagtctt
    36781 tctctgaaag aagtactttt atttttttta caaactggct tactgtattc actctcctga
    36841 catgatcagc aagattattc atatctcttt taataatctc atgctggaca tctgcgtcat
    36901 cttcttcagc tatagagaaa tatctatcta gtttattaga taatgcagag tatggctcta
    36961 ctacctcttg ctgcaaatta cagcctaatg cgtaatgtaa ttcagccatg aactcatcaa
    37021 atccattaat tttaataaga tgaaaatgag gaaagcgggc cagattttta agttgctcag
    37081 aaagtttttc agtgtctgag tcacggatac accaataaat cccatgagga aaacaactac
    37141 cggaatgcaa taacgtattg agagtatcca taattgaacg gtcgtgtccc gagtacccaa
    37201 taacgatcat gccaaattca gttgcaaact gtctaaattt ggccctcatg ttatcttcaa
    37261 gtgactcaag ctcccttata gtattcttga tattgtcaaa taaaaaatca ccatgtaatt
    37321 ttattatttt tggccgagct gtggttaaac gtatgctgtt gatacttgaa tcgtgggcac
    37381 acactattgg tctcagatta ttagaaaagg taaagcaagc ttcattgact aaatcatcga
    37441 agttagtcgt aaaaattgta ttaaaatggc ctttatccaa tagatttgtg agataaacgt
    37501 atccccaaga tggtttagct gcggtaatac aactttcaat aaattctctt ctttgagttg
    37561 gttgatcata taatgcttca aaaagctctg aatactcatt atctttatcg taccaaactt
    37621 gttttttgag agcatcttct ccatacatta gagtgtaagc atctctccag cgccttatca
    37681 tcccaccagc actatcaaca ccacttgaga tactagcacc tgcaccaaga aaaagagtaa
    37741 aatttggatg atgttgtttg gttgaagtca ggatgttaat taggtgctgc tgtggtctaa
    37801 ttttcatata tataccgaga cattccatga aagttatcat cggttccata tgatacacga
    37861 tatccagttg gttcgatcat tttcgcaaca ataaaatgat gcattccact atcttatact
    37921 ccctatgtta cccctaatgt taacgagaac ttaacacagt tgtctttcat gctcttgctc
    37981 ctttcagtcc gaacttagct ttgatttctg cgatcttcgc cagagcctgt gcacgattta
    38041 aaggtctacc gcccatgaca ggaagttgtt ttactggttc aggtatcgtc tcaccacggt
    38101 taattcgcgc tgtcatacag gtcagttcat cggcagcctt gcgccgtaat tccgcgtcag
    38161 tcagcgcatt ggcccgcatg ttctggtaca agttggtaac caaccagtag tgcgcgtttg
    38221 atttccatgg ataagactct gcgtccggat acaggccacg cttccggcaa tactcgtaaa
    38281 ccatatcaac cagctcgctg gcgtttggca gcccggcggt aacggatgtt tcttcccggc
    38341 accaggcaac aaactgcccg ggtgatggca ggaatggtcg attctgccga cgggctacgc
    38401 gcattcctgc gttaacctgt tccattgtgg tgatcccgtt ttcccggaaa gccagcaccc
    38461 actggcggcg gatttcgttc agttcgttct ggtcccggtt agccaggctc gccgggaaag
    38521 ttgccagtaa ctggctgaac acaccattga tgatctgcgc taccagctgt acctgcggct
    38581 tttcgtcgta ctgttccggc atgttattgg cgatccgacg catctgctca cgatcaaagt
    38641 taaccatctg tgcggcgatg tttttcataa atccacccca taaatccagt cagtgtttgt
    38701 caggtcgagt tttgattttc cggctgtcac gcctgcctgt tgcttgttac ggttgatttc
    38761 gagttgggtc cacttgtcgc ggagtttggc cggacttagc acgttaccgg accagaagtt
    38821 gtcctggcat gcccagcgga acagcacgca catgtcgcgg tggttacgtc cgtcacgttc
    38881 acgcatcagg cggatatcgt tagcccaccc tgcaaaattc ggttttctgg ctgatggcgc
    38941 gatggtcttc accatgtcaa acatccactc tgcggcggtc aggtcttctg ctgtccccca
    39001 cctgctaccg ctctgaattg cagcatctgg tttcaccaca ggaagatcgt tttctggctg
    39061 gtcagaggat tcgccagaat tctcggacga aaaaggtttt atattgtctt ttgttagttt
    39121 gtcttttgtg tttacctgat tcgggtaaac gcctttacct gatttgggta aacttttctt
    39181 acctgattca ggtaaattta cctctttcag gtaaacttta tttttcttac ctgattcggg
    39241 taatgttgac cattcactga ccacattatt aatgccgata ttccgcccgc tctgaataag
    39301 aatcccacgc tttaccagaa cgctttttgc agcagaacac ttgtgcggca atatcccggt
    39361 caattcggaa agttgctcgt tgctcaccca atccagtctt ttattaaagc catatgtttt
    39421 gcgcatgaca gccaggaaga ccagaagctg gtgctgtgtt aatccggcca gcatcacagc
    39481 ttccagcaac tcatttgcaa tgcgcgtata accatcgtcg agatctgcca cgcgccgctc
    39541 cttttgtgcc acatccggca ctggaaaatt gaatatctca gcagtgtttg ccataattcc
    39601 tcccgcaatg agtgtgttac gatttgcacc tgaaagtcgg ttctgttcgc gcagaccggc
    39661 tttcgccatt tccgaacctg tcatattgcc cccagcatgg tggtgaccat cgccatcagt
    39721 ggaccagcca aatccgggtc cacacgaaac atcgacacaa tgccttcact catctccttc
    39781 agtttctggt ggcgtggtgc gttgagaatg acagcctgtt ttgcctcact gagttccttt
    39841 tccatttcag ccagccgagc catgtagcta tcctgctcaa ccaggtggcc gcgatattcc
    39901 agcggtagta ccgccagaat tgccggggtc agttcacgca cgttatttcg gtatttttca
    39961 gaatcgaatt tgttatcgag gaagcggaac agcttctggc gtgcacggct gacatcatca
    40021 gggaaatcga tggtgccgcc gccctgctcc cgatactcat tcacaatgag tgcggcaacg
    40081 acatcctgat tatctacagc cgaccaggcg cggacggcat cacggatttt ttcgtggcct
    40141 ggcacctgtt ttgtttgaga acgatttatc accgcagtcg ggctaaatcc gctagtctgt
    40201 tggtatgtaa gtggttgcat aattgactcc tttagtttga attgactgtt aagttgattg
    40261 cttattgtta aagagcgtga aatggaaatt taagctgcgt tcttttcggt gtgtggaaac
    40321 aacttcggaa gatccgggcg aatctggtat gccttcacta ctccaccagt agccgtaaca
    40381 atgctgccga catgttcagg ggataccttt gctttgttgt gaagccactt atagacggcc
    40441 tgctgtgaaa cttcgcaagc agcgcccagt ttcttttgtg aaccaacgat attgatcgct
    40501 gttttgatag ctgggttcat aacaacctcc gtggttaatt tgaatcaaga ttaaaactat
    40561 ggttgttttt agtcaacaac cattttcgtt tgatggaata aaaccttggt tgtacatttg
    40621 gactatgaaa acaacactct cagaaagact taaagaagcc agattagcgc gaggccttac
    40681 acaaaaggcg cttggggatt tggtcggggt tagccaggct gctattcaga aaatcgaaac
    40741 agggaaagct aatcaaacaa ctaaaatcgt ggagatcgcg aacgctttgg gtgtgcgcgc
    40801 agaatggtta tcttctggcg ttggaaatat gtcagacagt acagtgcaac caatacaatc
    40861 aactgtcagc cattccaaat acttcaagat tgacgttctt gatatagaag tcagtgctgg
    40921 gccgggagtc atcaaccgtg agtttgtaga agttctacgc tcggttgagt actcgtttga
    40981 cgatgctcgt cacatgttcg atggtaggaa ggcggaaaat atccgcatca ttaacgtgcg
    41041 tggtgacagc atgtcaggaa cgatcgaacc aggtgatctg ctgttcgttg atatcacagt
    41101 taaatctttc gacggtgatg gtatctatgc gtttctgtac gacgacacag cccatgtaaa
    41161 gcgcctgcaa atgatgaagg ataagctgct ggtcatctct gataacaaaa gctactcacc
    41221 gtgggacccg atcgagaaag acgagatgaa ccgggtgttc atcttcggta aggttattgg
    41281 gagcatgccg cagacatata ggaagcatgg gtagtaccaa ttaaaaatta tcaactgggc
    41341 attgtgctca ttcagtaaag aactaattcc tatctttgct ctaggtagta atattaagcc
    41401 accgcaataa tatctttacc taacggcgta agaatcccgg tcaccgtgcc gggttttctt
    41461 ttgccctccc ctcatcacac acaccgttaa aaaaaccacc ataacctcgc ttcagttatc
    41521 gctatgcgat tcaagtcaca aaataaatcc atcctaaata caaccagtta tatctaaaac
    41581 aaccaataaa acaacttttg ttgttgacga taaaacaact atagttttaa ataagttcat
    41641 cgcaacaaca caacgatacg gcaactacct gattcaccgt tgcgatgacc gcttagatcc
    41701 gcagtttgaa tttcagcagg cttcggggag tgcgaggggt gaaacggacg cgtgaacgtc
    41761 ggtgtgacca gctgaaatca actcaacatt tcatacctta gtcgcttcaa cgaggcggct
    41821 tagttatggc aaccggcggc catccaccgc ctgaatacgc gcagaagtct ctatatgttc
    41881 agcagcccag cttacgggca ggagttttta tggttcatca acattacgga acgcagaccg
    41941 ttaatcgcgg cgcggtcatg ccaggaatgc tggtcaaaca caaagatggt acctggactg
    42001 catcagctaa tttacgcgga cggctttatc tgcatcgcgg catcgagcgc acttataccc
    42061 gtgatttgct cgtggaagtt tttctcgacg gacgcggcaa cggcctgaat cactaatccc
    42121 ctttcctgtt tacctaatca gcccggcatt tcgcaggcga tattttcaca gctatttcag
    42181 gagttcagcc atgaacgctt attacattca ggatcgtctt gaggctcaga gctgggcgcg
    42241 tcactaccag cagatcgccc gtgaagagaa agaggcagaa ctggcagacg acatggaaaa
    42301 aggcctgccc cagcacctgt ttgaatcgct atgcatcgat catttgcaac gccacggggc
    42361 cagcaaaaaa gccattaccc gtgcatttga tgacgatgtt gagtttcagg agcgcatggc
    42421 ggaacacatc cggtacatgg ttgaaaccat tgctcaccat caggttgata ttgattcaga
    42481 ggtataaaac gaatgagtac tgcactcgca acgctggctg ggaagctggc tgaacgtgtc
    42541 ggcatggatt ctgtcgaccc acaggaactg atcaccactc ttcgtcagac ggcatttaaa
    42601 ggtgatgcca gcgatgcgca gttcatcgca ttgttgatcg tcgccaacca gtacggcctt
    42661 aatccatgga cgaaagaaat ttacgccttt cctgataagc agaatggcat cgttccggtg
    42721 gtgggcgttg atggctggtc ccgcatcatc aatgaaaacc agcagtttga tggcatggac
    42781 tttgagcagg acaatgaatc ctgtacatgc cggatttacc gcaaggaccg taatcatccg
    42841 atctgcgtta ccgagtggat ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc
    42901 agagaaatta cggggccgtg gcagtcgcat cccaaacgga tgttacggca taaagccatg
    42961 attcagtgtg cccgtctggc cttcggattt gcgggtatct atgacaagga tgaagccgag
    43021 cgcattgtcg aaaataccgc atacactgca gaacgtcagc cggaacgcga catcactccg
    43081 gttaacgatg aaaccatgca ggagattaac actctgctga ttgccctgga caaaacatgg
    43141 gatgacgact tattgccgct ctgttcccag atatttcgcc gcgacattcg cgcatcgtca
    43201 gaactgacac aggccgaagc agtgaaagct cttggattcc tgaaacagaa agcctctgaa
    43261 cagaaggtgg ctgcatgaca ccggacatta tcctgcagcg taccgggatc gacgtgagag
    43321 ctgtcgaaca gggggatgat gcgtggcaca aattacggct cggcgtcatc accgcttcag
    43381 aagtttacaa tgtgatagca aaaccccgct ccggaaagaa atggcctgac atgaaaatgt
    43441 cctacttcca caccctgctg gctgaggttt gcaccggtgt ggctccggaa gttaacgcta
    43501 aggcgctggc ctggggaaaa cagtacgaga acgacgccag agccctgttt gagtttactt
    43561 ccggcgttaa tgttactgaa tccccgatca tctatcgcga cgaaagtatg cgtaccgcct
    43621 gttctcccga tggtttatgc agtgacggca atggccttga gctgaaatgc ccgtttacct
    43681 cccgggattt catgaagttc cggctcggtg gtttcgaggc cataaagtcg gcttacatgg
    43741 cccaggtgca gtacagcatg tgggtgacgc gaaaagatgc ctggtacttt gccaactatg
    43801 acccgcgcat gaagcgtgaa ggcctgcatt atgtcgtgat tgagcggaat gaaaagtaca
    43861 tggcgagttt tgacgagatg gtgccggagt tcatcgaaaa aatggacgag gcactggctg
    43921 aaattggttt tgtatttggg gagcaatggc gatgacgcat cctcacgata atatccgggt
    43981 aggcgcgatc actttcgtct actccgttac aaagcgaggc tgggtatttc ccggcctttc
    44041 tgttatccga aatcccctga aagcacagcg gctggctgag gagataaata ataaacgggg
    44101 gctgtatgac tgatttcacc ggaagcaata ctcctgccga acatcgcgac agctggcgca
    44161 caccaccaga gatttttgct gcgcttaatg cagagttcgt ttttcaactt gatgctgccg
    44221 ccagcgaaaa aaaccgacta tgtcggcttt ttatctcaca ggagcagaac acattaacca
    44281 cttcatggcc tgaagcaatg ggatatgcct ctggttatgt ctggttgaat ccaccataca
    44341 gcaatatttc cccttttgtg aaaaaggcag ccactgaaaa caaattcagt agtgtgggat
    44401 gtgtaatgtt attgcctgct gacacatctg tcggatggtt tcatgaagcg atacaaaccg
    44461 ccagtgaggt cagattcatc acggcaggac gactggcatt tattaaccca ctcactgaga
    44521 aacccgtcag tggaaataat aaaggctcga tgctcattat ctggcaccca tacccccgta
    44581 cacactgcca ctttacgacc gttgatcgtg gagagttgat ggcgttcggc tcaaggattc
    44641 ttgcccgtcg ggaggctgca tgacaaccac ggaatgcatt tttctggcag cgggcttcat
    44701 attctgtgtg cttatgcttg ccgacatggg acttgttcaa tgacacctca gcacgaaaac
    44761 gcccttcgca gcattgcccg tcaggctaat tctgaaatca aaaaagccag acagcatttt
    44821 ccggataaaa acgtcgatga catttgccgt agcgtactaa agaagcaccg cgaaacggta
    44881 acgctgatgg gattcacacc gactcattta agcctggcga tcggcatgtt gaacggcgtc
    44941 tttaaggaac ggtgaacatg aaaagcaaaa tcatcaggga gctacaggct ccttttttat
    45001 tattcgcatt taccctcaag cgtattaacc aacaattcag ggattaatga aagatggcag
    45061 acatcattga ttcagcatca gaaatcgaag aattacagcg caatacagca ataaaaatgc
    45121 gtcgtctgaa ctaccagact gtatccgcaa ctcattgttg tgagtgtggc gatccgatag
    45181 atgagcgaag acgcctggct gttcagggtt gtcggacttg tgcaagttgc caggaggaga
    45241 tcgaacttaa gaacaaacaa tggggactgt gatggcctca aagcagcaaa tttcaacatc
    45301 gtccaactga ggtgtaaaaa tgttcagaat catttttcct aacacctggt acgtcgacca
    45361 ccacggcact ccctgcaaaa tcctgcgttc tacccacaac aaagttcact acatccgaaa
    45421 aggcagaaca tgtatcgcca gcatgttccg ctttaatcat gactttgaac ctgtgaataa
    45481 agctgatgca gatcggatag cagaagagat cgaaacggca gaaaacatta agaagttacg
    45541 tgacatgcgt tcaaaaagca gaggtaacca tggaatcata cagcctcaca ctcgatgagg
    45601 cctgtcagtt tcttaagata tccagaccaa ccgccaccaa ctggatacga acaggccgcc
    45661 tacaggcaac acgtaaagat ccaaccaagc caaaatctcc ttacctcaca acacggcaag
    45721 cctgcattgc ggcgcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
    45781 taacagagga aagaaaatgt cactcttccg cagaaatgaa atatggtatg cctcgtattc
    45841 gctcccgggc gggaaacgaa ttaaggaatc tcttggcaca aaggacaagc ggcaagctca
    45901 ggagttgcac gacaagcgaa aagcagaact ctggcgagta gaaaagctag gggatttacc
    45961 tgatgtcact tttgaagagg cctgcctaag atggcttgag gaaaaagctg ataaaaaatc
    46021 tctcgattca gataaaagcc ggattgagtt ctggcttgaa cattttgagg gtataaggct
    46081 taaagatatc tcggaggcaa agatttactc tgctgtaagc agaatgcata acagaaagac
    46141 gaaagaaata tggaaacaga aagttcaggc cgccatcagg aaaggtaaag aactgcctgt
    46201 ttatgaacca aagccagtat caactcagac aaaggcaaag catcttgcca tgataaaggc
    46261 cattctccgt gctgcagaac gcgactggaa gtggctggaa aaagcgcctg tcatcaagat
    46321 accagcggtc agaaacaagc gagtcagatg gctggaaaag gaggaagcaa aacgccttat
    46381 tgatgagtgc cccgaaccac tgaaatctgt cgtcaagttt gcgctggcaa ctggtctgag
    46441 aaagtcgaac atcataaatc tggaatggca acaaatcgac atgcagcgac gagttgcctg
    46501 ggtgaatcca gaagagagca aatcaaaccg cgccattggt gtggcgctga acgataccgc
    46561 ctgtaaagtg ttgcgtgatc aaataggcaa gcatcacaaa tgggtgtttg tacataccaa
    46621 ggcggctaag cgagcagatg gaacatcaac gcctgcggtc aggaagatgc gcatcgacag
    46681 caagacatca tggctatcag cttgtcgtcg tgcaggaatt gaagatttcc gtttccatga
    46741 cctcagacac acctgggcaa gctggctgat tcagtcaggc gtcccattat cagtgcttca
    46801 ggaaatgggc ggatgggagt ccatagaaat ggttcgtagg tatgctcacc ttgcgcctaa
    46861 tcatttgaca gagcatgcga ggaaaataga cgacattttt ggtgataatg tcccaaatat
    46921 gtcccactct ggaattacgg aggatataaa gaaggcgtaa ctaattgaat tgtaatggcg
    46981 cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg
    47041 agctaagggc gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct
    47101 tgctgagtca atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc
    47161 tgatttattt cgctatagcg gcaaacaaac acacaccgct gcgcgtctga atcaagaaaa
    47221 cccgtatttt catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta
    47281 tgagactggt aactatgaaa ccaacgtcgg tgatcattat ggatactcat cctatcatca
    47341 gaatgtctat tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg
    47401 atgattaccg cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg
    47461 atatagactt gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga
    47521 gcacagtgaa agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga
    47581 tacaagctgg tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg
    47641 ccgttcagat gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa
    47701 aaagcaataa atgtagtacg aatagttcaa cggtcactgt gctatctaat cgtgaagtga
    47761 ccatattacg ttatctggtt agcggattat ctaataaaga aattgccgat aagttattac
    47821 ttagcaataa aacagttagt gcgcataaat ctaatattta tggcaagcta ggtttgcatt
    47881 caattgtaga gcttatcgac tacgccaaat tatacgaatt aatataatat taattataat
    47941 tgatcataaa tatcgcatcc gctttcgcca cacctggccg aacaccgctg gctaacgctc
    48001 gataattggc aaaaaagttt agtgtgacat tgccatttgc atctacttcc tcagtcgggc
    48061 tcgccgctcc cagtgcgagc cgggagcgat cgctattacg taattcgatg gcgatggttt
    48121 gcgccattgc gggatcatcc agagccagca ggttggtatc ggatgccggc gttcccgtaa
    48181 ataaaatcgc aactgaaccc ggaggacatc cctccagccg caggctaaaa gggacgagtg
    48241 ccgtggtatc gccagcgttc agtaattgtg tcgtaggcca tctgcctaaa tctaccgtct
    48301 tatcaatatc cgcagtgttt acggtacagg agaaatcaac aacgttaccg tgcaaattga
    48361 tattaatcgt tcccaaaggg tcagctgccc atccactgga aatccacagt aacccgcaga
    48421 aacagctaaa gagtgctctt ctcattctcc gtacctcatt gataatcgac gcgtaaatac
    48481 cccagcgcgc taaacggccc ttcggtcggt ttttgaccgg taatactgat aggccaggcg
    48541 cgaagtgtga cattggctgc cgcagctgca tccagacgga aaggaataac gctattgaga
    48601 tcgttaggcg tgatcggcgt atcgttctga tcggcgacaa taaaacctaa atcctgattg
    48661 tccgacacca tcgcctgacc agaaacggca ctggcttcca gacgcattgt taaataagcc
    48721 tgcgcagcaa cattcgtaca tttgaccgca atgctcttgg tttgcggcat gacaccagca
    48781 ggtcgattac ccggccctgc cgcactaaat aacgatgcgc cgatatcacc aaaatcaaat
    48841 tcaacaatct gcccggcatt taattcgcag ttttgcggta cttcaacccg gccaccaaaa
    48901 ctaatggtat aaacaggtgt aaccaacgga tcgtaaggcg ttgatgtcac atacacggta
    48961 aacatcacct gtctggggat ctccaccatg ttaataaagg gacgaataac ctttaaacga
    49021 aatattaaac gagaatccat caccccgaat ggaaggtttt gtgaaacgtt aggatctgtt
    49081 cccatccgaa tataattttg cggggggaac tgaatatcca tcacactatc aaccagactc
    49141 atcgcaccta atagataatc atttaattgc atatatttat aattatcgat agtttcctga
    49201 acaataaagt tggtgacata actacggtat gtataattca ccagcgtgcc gggtggacaa
    49261 attgctgaca cacctaccca gccggatttt tccggtaaaa caacaatctg ccctggctgg
    49321 ttattggtgc tattaaagat atttgagaga tcatagtgtt catcttctgc tacgccagta
    49381 ctatttgcac aaacagtagc ccaggatatg tttgccagac acaaacatgc catcgccaat
    49441 aataatctgc agattatctt cattgataat ttcctttatg agccaggatg tgtgcaaact
    49501 gccgaaataa cagtgacggc ttgctgtaag ctttgcttcg gcaatacata gtgcgcaatg
    49561 cagcgtgagt tcgcgccgtc tccccactga accagtaatt caccagacaa tggcgcacca
    49621 cttaaataaa cttgcccgtc atcacccacc atactggtta ttccggtact gttttcccgt
    49681 accagtgatc caaacggcac cggttttccg ccctgggtaa cggtaatgag cgcccgcacg
    49741 cctatgcggg tatcaaaatt ggcacgaacc aacgcgcctt gcgtcggcac aacgctgcta
    49801 atattttttt caacatcgat ggaattcccc atcgtattgg tatcaagcgc gatacggtta
    49861 taccgataaa ccgtggcata cggcatcacc gcatagccgc gccagtcggt taaaatgcca
    49921 gtttgatttt caatgcgtac accgcctgcg ccaggcgctt taatcaaaac attggtatcc
    49981 cctaaaggct ggctcagcgt tatgccattt tcgtgcccga ccacaccgcc agacagctgc
    50041 cagttaacgt catgttgatc gcggtcgtag ttatacccga cccccagcgt accgtaagtg
    50101 gcctgccagt ttgccgtggc gctgcccgta tacccattat tcgaggtatc accctggctt
    50161 acgtgataac tcaggttgtg gccttccagt aaggtcccac ctacacctgc cagccagcta
    50221 ttttgcccgt tgctgttacg gttggcgtta aaggaggcat aagcgcggtc gagcgcattt
    50281 tcccgggtgt agcgacgttt ggtcaaaaca ttgaaaggca ctgaaacatt aagtccgaca
    50341 atacgttcgt tatcggggat ccctacagat tcattccacg aaaatgagag cgaataactg
    50401 atgccaaccc agctgctggt ataccccacc tgataccagg tatctgaatc cgaagtattc
    50461 cagtattttt gatgggtacc agagatataa agcgagccaa agtcattaag tgattgtgaa
    50521 atatttaact gcaaacggtc tttacggcta aagcgtaaat tgtggtaatt cacgattatc
    50581 ggttcatcgc gatgctcacc gtcgtaatcg taatcatatt cgtacccctc cattcgacga
    50641 tacgcaacat catcaagggt ataaaaacct tgtgtcgaat agcggtaacc cattaactga
    50701 aaattggtgc cgaaggtgtt catcgatttc gcatagagga agcgaatgga atccccctca
    50761 tgacgactgt cgtcggctaa ctgactgcgc gcatgggtca catccagcga cactgccccc
    50821 cagttcccga ggttgcgccc cagccctaat aaaaaggcgg tgtaattggc agataattgc
    50881 gtcccgccgt aggcagtaaa ttcctgtggt aaaccgccga gtgccgtgcc ctgaaaaaag
    50941 aaaggcgatg attgctgact attaccgcta cgaaaatcgc ccgccgtcag gtcaaatttg
    51001 aaacgccctt cgcgttgtaa aattggcact gttgaatacg ggatagtgta attctgctga
    51061 ttaccatcgc gctcgtcgat ggtgacgtcc agatcgccat ttgaagatgt cgggtgcaaa
    51121 tctgtaattt caaaagcgcc gggggaaaca tagctttgat agataataaa accattttgc
    51181 cgaatcgtta gctgggccgc cgtacgggca atcccacgta ccgttggggc aaacccttgc
    51241 tggctatcag gatacatatt atcggaagaa taaagccgta caccacgaaa tccaacacca
    51301 tcgaaaatat cacttcctgt attgccgtct cccattacca gttcactttt cagcggaata
    51361 atggcgcgct gtacccaggt gccaatatta ttccactgtt ctgaatgata tccatttccg
    51421 cgaaaatagt tccaggaacc attgttgcgt aaacgccacg ggccaatatt aatcccgctg
    51481 agctcactaa aaaaatagct atcgttacca ttacctctgt taccggtgaa attataattc
    51541 aggagtaaag caggaatacc ttcatcccac tcttcaggcg gaatgtaacc gtgagcgcta
    51601 ctcaacaacg ttatctgagg aatagtgatg ttcaggcgca tcgctgcaaa atcaaagtta
    51661 attgtggcat caggtacagc tttcagtaaa ttgatgcatt tattgttttg ctgctgggct
    51721 aactcgggaa atgcactgct attaaggcca attcgttcaa ggagtacctg gttaaaacag
    51781 ggcattaacc cacctgattt atcacctgta ttttctgttg tcgattcaaa tacgatatcc
    51841 tgtgaaccaa tgaactcatc attacgccag agatccaccc gatacactcc cgcaggttga
    51901 tgattgcctc tctcaaagcg cgataaatca gcaacggatg tgccattctc taataaaaat
    51961 gccgggttga aataactttc cgcatagctt aaaaaggagg gtaaaatggc aagagatgaa
    52021 taacaaattc cggccagaca ccaggtatac ctctgcggaa tatccgtagt agtgggtatt
    52081 ttcataatta gctctgatat tgtcagatga ttcggttagt ttaagttcac ttctctgacc
    52141 ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg cactcctgag
    52201 ggtaagggaa tttgtgaatt aactctgggt gcagccatag cattccccaa cttttggctg
    52261 cccacttgta agttaaccag ggtgacataa aaaggtgagg gattatgaac attgatatag
    52321 ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc ttgtaattga
    52381 attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac attcctgccg
    52441 ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa cagagactca
    52501 cgatctgccg ccagcggtgg accggtgtaa ataatacgca aggtattttc gcttttgggt
    52561 tcactaacaa acagtggcgg tgtaatgatg aatgactttt cttttacacc gctgctgttt
    52621 tcaatccacg aattgaccag aaagcgctca ttggtatggc tatttctaat ccataccgca
    52681 gtctgttgag catcagcggg ataaataata cgcgtggcac ctaatgcgat ccctccggca
    52741 gcatgggcgc tggccgaaat gattaaataa aatataatga gcatcaataa ctttatttta
    52801 gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aaataagaaa tataaagaaa
    52861 tgattgcttt ggtttcttac aacacatcat tcccttttca tttaataaag cggcatgata
    52921 caaagtgaac atgatttcga tttacattgc caccataccg cttcagacaa ccggtacatt
    52981 actttgttat aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc
    53041 ataataaaag tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta
    53101 taacgtgcag aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag
    53161 ctattcccat caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc
    53221 gttgctgttg ttgtattggt actggatgca atggccaata aattatcatt gattgcatca
    53281 gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac
    53341 tgaatggtga atggaattaa tgcagaggta ttaccaacag cattgaaaat atcgatacga
    53401 tattgaccga gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc
    53461 accagttcgc cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta
    53521 gcggcgaata gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct
    53581 tcaatctcat ttatcgactc cacatccgta tataaccgat tactttattt aagacactga
    53641 tagtagtaaa attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt
    53701 ctaaaaaata gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat
    53761 atagacctga agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct
    53821 taaattatct taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat
    53881 ggacgaaaaa tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca
    53941 agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca
    54001 aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa
    54061 agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc
    54121 aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta
    54181 accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg
    54241 tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg gagcttatcg
    54301 atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg
    54361 gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc
    54421 atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc
    54481 gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg
    54541 tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg ctcgcaggtt
    54601 gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta gaaaatgccg
    54661 atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg atcaaagaag
    54721 gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg
    54781 tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg
    54841 gcccgatgac ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg
    54901 atccacagga tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg
    54961 tgcgacttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt
    55021 gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc
    55081 gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg atccctttcc
    55141 cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc
    55201 catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg
    55261 ccgctgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt
    55321 tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg tttaaatttg
    55381 gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt
    55441 ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct gggctgattc
    55501 gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat tcggtaacca
    55561 ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct
    55621 ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg tacgggcatc
    55681 aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt atgggaatgt
    55741 tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag cgcaattact
    55801 tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac
    55861 ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt
    55921 gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga
    55981 acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg
    56041 ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca cgcgccatat
    56101 caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc
    56161 gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag
    56221 tgtagagacg ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcaact
    56281 ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca
    56341 gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca
    56401 tcaccatccc cgagtgcatc cagtagttca catactggcc atcatgggca caggtggact
    56461 gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa
    56521 agtggttacc cagctgcttg cagttcattg ccgaacattc aatgtgccag cctggacggc
    56581 ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga
    56641 agtccatcgg gttgcgtttg tcgtcgacca cgtcaacgcg cgcacctgcc tgcagctggt
    56701 cgagatcctg acgcgacagc acgccatatg tcggatcggt cgggacgtcg aacatcacgt
    56761 cgccgttgtc cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa
    56821 tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat
    56881 caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag ctttcgccat
    56941 tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca
    57001 gtttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc
    57061 cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacatacatg ccgacttccc
    57121 cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca
    57181 tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc
    57241 gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc
    57301 tatatatgac ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt
    57361 actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa agcacctgaa
    57421 acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac
    57481 cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat gaaacaaaaa
    57541 gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac ccgtggtacg
    57601 ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg
    57661 gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg
    57721 tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt
    57781 cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt
    57841 agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg
    57901 cgatcaccgc cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt
    57961 atattcttgg cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc
    58021 gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc
    58081 atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat
    58141 tgccggaaga aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca
    58201 cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc
    58261 tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc
    58321 gagcgaacag taaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa
    58381 acgcggtagt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac ggacataccc
    58441 atcgcccggc ggtacatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactcg
    58501 gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc
    58561 attttccgtt ttaaaaaacc cgcaactttg ctgatttcac agccacgcaa ccgttttcct
    58621 tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag
    58681 ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca
    58741 aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg aatgtcccgc
    58801 accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa
    58861 gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca gcgcacctgc
    58921 caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg
    58981 cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg
    59041 gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc
    59101 tcgcgactca cgataaagag ctacaccagc gtctaaatga ctggcgcaaa gcccagaccg
    59161 acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg
    59221 taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg
    59281 gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc
    59341 tgagatcgaa cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt
    59401 tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc agcttttcga
    59461 taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc
    59521 ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg
    59581 tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg
    59641 cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg ttggcgcgcg
    59701 cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat
    59761 tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga
    59821 gatgctgtcg gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt
    59881 tgtcaccccg caaggtctgt tgatcaacga actggcaccg cgtgtgcata acagcggtca
    59941 ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga
    60001 tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag
    60061 cgatgtgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga
    60121 agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct
    60181 gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat
    60241 ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa
    60301 cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt
    60361 cgcatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc
    60421 cctgcttcgc ccgccagcgt atcttcaatc cgcgataacg ccccaatcca cgcgggttta
    60481 ccacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct
    60541 ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcgcgttg ctgcggcgtt
    60601 ccccagtttt catataccgc atcagcatcg gtgaggatca ccagcccatc ggcgttaatc
    60661 tgctcggcaa gcaaggcagc ggcgagatct ttatcaatca ccgcttcact ccctgccccg
    60721 tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc
    60781 aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccgg
    60841 cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt
    60901 tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca
    60961 ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc
    61021 aagctctgcg ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc
    61081 ggatacggtt ctaccttttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc
    61141 ccgttgccgt gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca
    61201 gcactggcga tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag
    61261 gcgttgcccc caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc
    61321 caagccagaa ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat
    61381 caatcgcaac agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg
    61441 aggcgaaata tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga
    61501 gttgaagatt gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat
    61561 aataccaggc cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg
    61621 ggcctttacc cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac
    61681 agtgacgaaa ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggtc
    61741 cgaaaagtcc ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg
    61801 aaggcggcgt aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga
    61861 ggcgaatccc ttgagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg
    61921 catcgaattg cgcgcggcga agcacccatc ctccggggcc gaaaccacta ccctgacgat
    61981 gcagcgttaa cagttcgccg ctttccgtca tcaggttaat cgcccgtcgc cacactcccg
    62041 ctaaccgcca ggactggcga taattcggtg cactacggct ggcaagcaga ggatggatga
    62101 tcgtcattgc gcgttcctca accaatcccc atgctttcag ccagtgcttc cagcgcctgt
    62161 tcaaaacacg ccagcggtgc tcgcacagtg cctgcgccaa tctgcccgat ccccgcctct
    62221 ttatgggcga taccggtatt gatgagtggc gtaatgccgg tttctaccac gcgacgaatg
    62281 tccagtccca ggcacgcgcc ctgaaaatcc cagcctggga tctgcaactg catattgcgc
    62341 tcaaggtaaa tttccgccat ctcttcagat accgctctgg ccgcttccat gccacccgca
    62401 ccgacaaagc gcgttacgcc aggcgctgcg atcattgccg cacctccgat accaaaggtt
    62461 tcggtaatcg cgctatcgcc catatccggg ttcgcctgct cctgcgagaa gccggtgaaa
    62521 aacagacctt gcggagtgtt tacaggcgca gtaaaccagc gttcgcccag cccgcttacc
    62581 cgaatcccga acatattgcc gttgcgggtc attgccgtga cgatgctgcc tgcgcggatc
    62641 atcgcgccag catccatcgc cgccttgcag taagccatcg cgaggttgag gaagaactga
    62701 tcggtcacgc tgaggaaatc catcacttcg gcgatgtgct gtttatcatg atcgaggcga
    62761 gcaatttgtg gggccagcgc acgcattaac agtgcagagg aagcaatatt gcgttgatgg
    62821 aactcatcgc ccatcgtaat gccctgcgcc atcatcgccg tgagatcgat accgcgctcc
    62881 atgcgcccca gcgccgcgct taatactggc attaacacat cgcgcatcca gcgatggcga
    62941 gtcaggacat cttcgccgta agcgccaaaa cgcatcactt tgccgatacc ttcgttgagg
    63001 ttgcagtacg cccggttgcc gtcggtcacg ttctcgacca ccagcatcgg catactggca
    63061 gaagtaatgc cgcccattgg cccgacggca ttcacatggt gacaaggaat gaagttcact
    63121 tccccctgct ccagtattgc cagcgcctgc gcttcatctt tcgcccagcc ttcgaacaga
    63181 catgcgccca cgcacgcccc tttcatgggt ccggtcatct cctgccagcg cattggcggc
    63241 ccggcgtgaa gcagtgtttt gccctcgttt agttcgctga taagtgaaga agccggttgc
    63301 acatccagcc agtgtggacg agcgcgacga atttgttcga taaccgcagc attggcttgc
    63361 gccactgatg taaacatggg aaccccttat tgcaaacgtt ctaataaacg agccagtttt
    63421 ttattgccgc cggcgactgg cgaccattgg taatgcacaa ccggtttgct ggcgctttgt
    63481 agctccagcg caaagctgcg taatccgatg ttaatcacgg cgacgttttc cagtaatgac
    63541 ggtgtgtgtt gctgagtggc aggcgaaagc ggacgaatca gtgccgccgc cagcaaggtg
    63601 gcttccggca gcgaactcac taccgcgatc cctgcatctt ccagcgtggc gatttgctgc
    63661 gagcggcatt gcgggtcacg ttcagtgcct gtcaccgtgg caatggcata cagtggttga
    63721 ttgtctgaac gcgcggcaca tgctttttgc caggcgctta ccagcgaggc ggctggatcg
    63781 gcggtcgcac cgaagccaat cacgacatca agcagcaata cgcgcacttg cggtttagcg
    63841 ccgagatcgg caattaactg attgcgtaag gctgggtcga tcatcggatg gggacgcccg
    63901 acggtgtaga aatcatcgcc gaggtcaata atctggtgac catcggcgtc gagcatcatg
    63961 ccgtgatgat gggtatcgtc ggcttccacg ccaaggtgtc cagcaagtaa tcccgccgct
    64021 tcggcagcca gcgtaccgcc ggtatacaaa ccgcaaataa atccgctgct ggcaggcgtt
    64081 atcgcgttac gtcgcgccgt aacgcgtgaa agcaggcaag ccaggcgtgc ggcctcatcc
    64141 agcgaggagg caaaccagac attctcgtcg cgtgccaccg ccggggtata acctaaaaac
    64201 agcgccaccg tcggtttgcc ggttgctttc atggcattaa caattttcag acgcacagct
    64261 tcggcaggtg gttttgaaac aaatgccagc acttcgcttt tctcgtctgc actgagcatt
    64321 tccagcgctg ttagcgcact gatgccgccc acttcacggc tgaggtcgcg cccgccaagg
    64381 ccaatcgcgt gagtaattcc ctcccctgcc agcgcaatct gcgaacacag ctcctgaatc
    64441 ccggtaccgg aagcgccaat gacgccaata ttgccttctg gcatcacgtt agcaaaagcc
    64501 agcggtgtgc cggcaatcat cgacgtaccg cagtccggcc ccatcaccag caagcctttt
    64561 tcccgcgcgc gggttttaag ttggatttca tcttccagcg tgacgttatc ggagaacatc
    64621 atcacgttga ggttgcgatc cagcgcctgg tttgtcagct ccgccgcata ctcgccagcc
    64681 actgaaatca gcgccagatt ggcatcggat aatttctgac aggcactgtc ccagcgacgc
    64741 acctgcgtca aggactggct gctgcctgac ccctgcgcca gttgttttag cgcctcttca
    64801 agctgctgca taatcgcctg cgcgatcccc gcatccgccg cttcgctacg tattgccacg
    64861 caaatatcgt tcggcgtggc gttattaaaa tcgtcatgcc agaaacctgt ggtatctaat
    64921 aacgctttat tggcgggcgt acccatcatt acggaaacat catcaacatt ttctgattcg
    64981 ctgagttttc gtgaaataat cattaaactg accgaatcct gaaaacaccc ttttttaata
    65041 aaggcgtgga tcataacaac tccttaaaaa ttgcacccat ggcaattgca cgagcagtgt
    65101 taatgtggcg attacggtat cgatgacaac ggataaataa ggtgatgaat atcacacagg
    65161 gattattaac aaaagaattc aaataaccgc catgctatta cgtgttattt tttaataagc
    65221 gtggttgttt taaaaaccag gttaaaagcg agcggattat caaataaatt ctaactatat
    65281 ttttttgcct gtctggatca cataatccag atattttccc tgtatgttaa ttgcagtcat
    65341 gcttcacacc gtcgttaaaa aggaagacag atgaaaatca gtcgggaaac actccaccag
    65401 ctaattgaga ataaactctg ccaggctggg ttaaaacgtg agcacgctgc aaccgtggct
    65461 gaagtattgg tttacgccga tgccagaggg atccactctc atggcgcggt gcgcgtggaa
    65521 tactacgcgg aacgcatttc aaaaggcggc accaaccgcg aaccggagtt tcgtcttgag
    65581 gaaaccgggc cgtgctcggc aattttacat gccgacaatg ccgccggaca ggtcgcggcg
    65641 aaaatgggta tggaacatgc catcaaaacc gcccagcaaa atggcgttgc ggtggtcggt
    65701 atcagccgga tgggtcacag cggcgcaatc tcttattttg tgcagcaggc agcccgcgcc
    65761 ggattaattg gtatttcgat gtgccagtcc gatccaatgg tggtgccgtt tggcggcgcg
    65821 gaaatttact acggtactaa cccactggcc tttgccgcgc cgggagaagg cgacgagatc
    65881 cttacctttg atatggcgac taccgtacag gcatggggaa aagtgctcga cgcccgctcg
    65941 cgtaatatgt ctatcccgga tacctgggcg gtcgataaaa acggtgcacc aacaaccgat
    66001 ccgttcgcgg tacatgctct gctccccgcc gccgggccaa aagggtatgg cctgatgatg
    66061 atgattgacg tcctctcagg cgtcttactc ggcttaccgt tcgggcgaca ggttagttcg
    66121 atgtatgacg atttacacgc cgggcgtaat ttggggcaat tacatatcgt tattaacccg
    66181 aactttttct tctccagcaa attatttcgt caacatctta gccagaccat gcgcgaatta
    66241 aatgccatta cccccgcgcc cggttttaat caggtttatt atcccggaca ggatcaggat
    66301 attaaacaac gcaaagccgc cgtcgaaggc atcgaaattg ttgatgatat ttaccagtat
    66361 ttaatttccg acgcgcttta taacacgtca tacgaaacga aaaatccctt tgcgcaataa
    66421 ttattgagac aggactttct tatgattaca cattttcgtc aagccataga agaaacgctg
    66481 ccctggcttt cctcttttgg cgctgaccca acgggtggta tgacccgttt actttattcg
    66541 ccggaatggc tggaaactca gcagcaattt aaaaaaagaa tggcagcaag cgggctggaa
    66601 acacgtttcg atgaagtggg gaatttatat ggtcgcctga gtggcaccga atatccacaa
    66661 gaagtggttc tgagcggttc gcatatcgat accgtggtta acggcggtaa cctcgacggg
    66721 caattcggcg cgctggcggc gtggctggca attgactggc tgaaaacgca atacggcgcg
    66781 ccgctacgta cggtcgaagt ggtggcgatg gcagaagaag aaggcagccg cttcccgtat
    66841 gtcttctggg gcagtaaaaa tatctttggg ctggcgaatc ctgacgacgt gcggaatatc
    66901 tgtgatgcca aaggaaatag ttttgtcgat gcgatgaagg cttgcggatt tactcttccg
    66961 aacgccccac taactccgcg tcaggatatt aaagcctttg ttgaactgca tattgaacag
    67021 ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg tgaatgcaat tgtcgggcag
    67081 cgtcgttata cggtaacgct gaacggcgaa tcaaaccatg caggcaccac gccgatgggt
    67141 tatcgtcgtg atacagttta cgctttcagt cgcatttgcc atcagtcggt cgaaaaagcg
    67201 aaaaggatgg gcgatccgct ggttctgacc tttggcaaag tagagccgcg cccgaatacg
    67261 gtaaatgtgg tgccgggtaa aaccacgttc accattgatt gtcgtcatac cgacgctgcc
    67321 gtgctgcgcg atttcaccca acagttagaa aacgacatgc gggcgatttg cgatgaaatg
    67381 gacattggta ttgatatcga tttatggatg gacgaagaac ccgtgccgat gaataaggag
    67441 ctggtcgcca ccctgacaga attgtgtgaa agagaaaaac tgaattaccg ggtgatgcac
    67501 agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg taccaacctg catgattttc
    67561 attcccagca tcaacgggat cagccataac ccggcggaac gcaccaatat taccgacctt
    67621 gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac ttgcctggca gaaataagga
    67681 gtcataaatg ggatatttaa ataacgtcac cggttaccgc gatgatttac tggctaaccg
    67741 tgcgattgtt aaacacggta atttcgcact gttaaccccg gatggtctgg taaaaaatat
    67801 tattccgggc tttgaaaatt gtgacgcgac aatcctctcc acgccaaagc tgggtgcctc
    67861 ttttgttgat tatctggtca cactgcatca aaacggtggc aaccaacagg gcttcggtgg
    67921 cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat atcactgcca aagccgaagg
    67981 caaaacattt gccttaagcg aaggtggcta tctttattgc ccaccaggct ccttaatgac
    68041 gtttgttaac gcccaggccg aagacagcca aatcttttta tataagcgtc gctatgttcc
    68101 ggtagaaggc catgcaccgt ggctggtttc tggcaatgcc agcgaactgg aacgcattca
    68161 ctatgaaggt atggatgatg tgatcctgct ggattttctg cccaaagagt taggttttga
    68221 tatgaacatg catatcctct cttttgcacc aggtgccagc cacggttata tcgaaacaca
    68281 tgttcaggaa cacggtgcct atattctttc cggtcagggg gtttataacc tcgacaataa
    68341 ctggatcccg gtgaaaaaag gcgattacat ctttatgggc gcttattctt tacaggctgg
    68401 ttatggcgta gggcgtggtg aagcgttcag ctatatttac tcgaaagatt gcaaccgcga
    68461 cgtagagatt taagtcatac cactcgcctg attattttta gctatgttgg attttgccac
    68521 ggtttatacc gtggcttttt ttgtagtaaa ttcaggtaaa aaaagatgcg aaataaatcg
    68581 catccgcttt aaaggtaagg gtcagttttt aattccctga cctattttaa tggcgcaggc
    68641 aatatttcgc gcgctgttga agagattggt ttcaccgctg gcgagcactt cggctaaagg
    68701 tgccagacga ggcaaaatgc tgaataccgc gtcaatgccg tactggtgca ccacttccac
    68761 gccatcaccc aatacgccag caatcccaat caccgggaca ttaaactgct tcgccaccga
    68821 cgcaacgccc agcggcgctt taccgcctgc cgtttgcgag tcaatgcgcc cttccccggt
    68881 gatcaccagt gctgcgccct gcaccgcctg ctccaggttg accgcccgca gtacaatctc
    68941 aatacccggt ttaatatccg cattgaggaa taccgccgcc gcaataccca taccgcccgc
    69001 agcgccgccg cccgccatct ggcagacatt aatttcagtt tgctgttgta aaacacgggc
    69061 gtaattttgc agcccctgtt caagttcttc gaccatctcc ggcgttgccc ctttttgcgg
    69121 gccaaacacc gccgccgcgc cgcgtgcccc taccagcggg ttatcaacgt cacatgctac
    69181 ttcaatatgg caattcgcca gacgcggatc gcattcatcc atctcaatgc ttgccacgcg
    69241 cgctaaatta ccgccgttag ccgccagcac ctggccgtcg gcatcaagga aacgcacacc
    69301 aagcgcctga gccatgccca taccgccgtc gaccgtcgca ctgccgccaa tgccgagaat
    69361 aatatggcga atgccgttat ccagcgcatg acgaattaac tcccccgtac caaaactgga
    69421 ggccagcaac ggattacgct tttcaggcgc gaccagcatc aggccacttg ctgccgccat
    69481 ctcaatcacc gccgttttcc cgtcgccggt aaggccataa aaagcattca ctgtttcgcc
    69541 catcggcccg cagacttcaa gcgtcacgat gttgccgccc gtcgcggcga ccatcgcatc
    69601 caccgtccct tcgccgccat ccgctatcgg caaacagata tagttcgcat cgggaaagag
    69661 ggtcgaaaac ccggctttaa ttgcctgaca acatttttct gcacttaagc tctctttaaa
    69721 agagtctggc gcaatgacaa tcttcattat caatcctttt tcaacaaatt attcgatgcg
    69781 ttcccacgga ataagattat ccataagaac agccagtaaa attccgacca gtaagccgtt
    69841 actgagcaga ggacgcagcg ttaatggaag gtcttgcaga tacacaggcg gtaatgccat
    69901 taaaaatatg ccgacaaata acggcaatgc gagtcgataa atattgcgag cggtaaaggt
    69961 tatttgctgg ctaaacacca gcgcggaaaa gagtaaaggc agataagaaa ccagcatgac
    70021 cgcgctactc acgggtaaag ggatactgca aaacagtcgt gtgagtgccg gaaccagcgc
    70081 caccagcagg caaataacgc tgccataaat aaacgaacgc cgcgtgtaat cgcctgtctg
    70141 ggttaataaa ccaatggatg aaacaaacgg tgaaaatgga attaccgcca gcggtacggt
    70201 tatcagcgtc ataaatccgg tcgccacaaa gctgcgacga taacgcgtat tccccgcgcc
    70261 ctgctgcgga taaaaaacat ccgtgccccg aatcgcaccg taggtattgc tgatattcac
    70321 cagacctgta atcaccgccg tcagaataat tcccggagac aaagcacctc cactgccgag
    70381 cgggaaccac tgccagtgca actcaccgga gagcgagtgc gaagaaggaa agcaaaagta
    70441 ccacaacaac cagccggtta tggtgccgac cagcaggcca taacgggcaa aacgttgcgg
    70501 caggaagata atcatcccca gtaccaggca catcaccgcc accgagagcg cgaacggcgg
    70561 taactgaatt ttaaaatttg ggtcggctat gccaaacggc agcccgagca tacctttgaa
    70621 aaagatagtg gtcagctgcg cgcccagcat caacataaac aagaccatca ccgacggcgt
    70681 aaataaccgt gccaggcgat ggcctaatcc gctaaaacca atcaacatcg tcagcacgcc
    70741 ggagagcgca atccccaccg ccaggctggt agcgatatcg ttgatcggtg tcccgcgcga
    70801 tgcttcgcca agggtgatag taaggatggt tccccaccac aggccacccg gcccttccat
    70861 aatagcgcga cgatgaccgc agaacgcttg agcgaagcag gccagtgcgg tagcaagaaa
    70921 agcatattgc gtgagcgtaa gcaggctact ttgcggcaac tgaaaagcag aaagtagcgt
    70981 aggaggaacc acaaccgtgt tgcaaaaaat aaagaaaaac cactgaaatc ctgataacag
    71041 gctttcgcgg ctcactgcaa aattgaacat aaacacacct taaccggaga atgcccgccg
    71101 caaggacggg cattgcaggg gccagattac tgctgatgtt taaggataaa ttgacctttc
    71161 ggcgcaacag ggaagccctg ttcaatgtcg taaatcacat caccacgtaa gatggttttc
    71221 gtgatacgcg cgccaatggt acggccaaca tacgggctga ctttgtggcg atattccaga
    71281 tcgtcattgg taagaacata gctgctattc ggctgaatga agacgaagtc ggcatctttt
    71341 cctggggcga tacggccttt ttgctgcaga ccgaaaatat ctgctgcgtt agtcgccatt
    71401 aatttgccga acattggcag agacattccg cgtttctgta ccgcttcatc gaacatcacg
    71461 tccatacagt tttgcagacc ggcaataccg ccccatgctt ccatgatgtt gccggctttc
    71521 atttccggag ggcatggtga gtggtcggaa accaggcagt cgatttcacc gttaaacagt
    71581 ttttcccaca tgcctttctg gttttccaga tcgcggatcg gcggtgaaca cttcgccaga
    71641 gtgccgattt cttcgaactg atcggtatcc agtacaaagt aatgcgggca ggattcacaa
    71701 gtaacgtcct gaccttcctg acgtgcacga gtcacttctt caacgccttc cgggctgctg
    71761 atatggcaaa cgtgcagacg gcaaccggca actttcgcca gatacagcac gcggcgaatc
    71821 gcttccactt cggtaaatac cggacgcgaa gccacatagt catgggcagt tacgcgacct
    71881 tcacgtttcg cttcttcgcc cagtgcgtca cagatcagcg cgttttcgca gtgcaccagc
    71941 accggctgcc ccagttcgcc cagcttctgc gcacctttga agaactgcca gtcgtttaca
    72001 tcacggaagt cgttgtcgat accgcgatcg ccacaggtcg caacgaagca tttgaagccg
    72061 acaacgccca cttcatccag ctcatgcaga cggtcgatgt tgtaagacac taggccaccg
    72121 agttgtgccg catcgatagt cagcttgcct ttagcggcat cgaacttcag ttcaattgaa
    72181 gcgcggtcaa ccgttgcagg cagctggttg agcggcattt cgatcatggt ggtgatacca
    72241 ccttttgccg ctgcgcgagt accggtttca taaccttccc agtggctacg acccggttca
    72301 gaaatatggg tgtgcgcatc aaccatgccc ggcgaaacca ccagaccaga cgcatccata
    72361 acttcttttg catcgcccag atcctgaccg atagcagcaa ttttcccgcc tttaacggcg
    72421 atatctacaa cgcgagcttc gttttctaaa ataacggtgc cgtttttaat gattaaatca
    72481 aaagacataa caaactcctt gtaaaataca gattggtttt taactcaatg ttcaatatcg
    72541 ggattaatta acctatggtt ttttgctctc ctgttttttc tgctgttgta cgtttcttta
    72601 ataaggcgta ggccgcaaag gcgacgatga cgccgacaaa ccatgaaaca cgcgataacg
    72661 gttccataaa gggaataaac ttaccgccaa gagataaaat aacggccacc agagttactg
    72721 aaaacgcggt gaggttaaaa ccgttatcgt aatatttata atcgccaggt gcggtgtaca
    72781 gttcatcaag attaatttgc ccacgcatca ccacaaaata atgtgccatc atgacaccaa
    72841 ttaccggacc cagcattccg ccgatgatat cgaggaacag ataaatactg tcctgatttt
    72901 ccattaattt ccacgggcag atcagcaggc tgataatact ggcaatcagt acgccgtttt
    72961 tataggtcag ttttgtcggt gcaatggcag caatctgata accggccgga ataatattac
    73021 cggtggcgtt agtggaaata gtcgtcatca gaataaccag taccgcaaag aacgaggcaa
    73081 acaggctgtc ccaacgctga acaatatcca gcacgttcca ggtgtcagcg ccgtagtgaa
    73141 tactggctcc ggcaataata cataccccag cgaccgcaaa aagaatatag gccacaacta
    73201 aacccagcgt ttgccccagc gcttgctcat gaaacgagtg ggcgttttgc gtaaagtcgg
    73261 atgcgctcac cgccggtgcc gcccagaccg caactaccgc gttaatcacc accaggaaca
    73321 agaatccact gttttctgct ttctgaatac cgctcggaat gtagtcaaag attggaccga
    73381 gcccgaccag cgaaatcgcc caaatcgcca taccgccgaa aacgatatag atgcacgggt
    73441 taagaatggc agtgaattta tttaaaactt tgccaccgcc aaaacctata ccaacgttga
    73501 ccagccagaa gagtaagaaa gtaattaagc ccggtagaga aagccctaac agcgtgaaat
    73561 caccaccgag agttaaaaat cccggccaga ttttgccaat cagaatcaag caggccagtg
    73621 accccgcgta acattgcagg ccaaaccaca taatggcagc aataccgcct cttaataatc
    73681 cgggaaacag tgcgccacgt acgccgtaag aagcacgcag gatcatggca aacggtacgc
    73741 cgtatttact gcccgcagcg ccgtttaata ccattaccgc ggcaatgaaa aaggcgctga
    73801 ggataattgc cagcataata ctaaaggtag acaagccgag aataaaaaag ccgccgacca
    73861 tcacataatt gggaacgtta tgaaccgaac ccatccataa ggtaaaatag ttaaatgttt
    73921 tccaggtccg ctggctttgc gttttcggca atagatcttc gctatagccg cgttgctgga
    73981 atagttttct ctgatgttcc ataaatccct cttaatttca cgtatcgaat aacgttgttt
    74041 taatctagct atgaggaaca aataaaataa aaatattatt tttaatactg aagcaacatc
    74101 tttggctgct ttctttttta atattgctgg ccgattcatt ttctgatatc ggccattttt
    74161 attgcgggta tcaggccagt ttatggttag ccattaattc cagcgcctgc actaacgcag
    74221 agtgatccaa ctggctgcca ccgtttgccg cacaggtatt aaataactcc tggcaggtcg
    74281 cagtgtttgg caggttcagc gcaagtgctt tcgcactttg cagtgccagg ttgagatctt
    74341 tctggtgcag agcgattttg aagcccggat taaaggtgcg tttaatcata cgctcgccat
    74401 gaacttccag aatacgtgag gaagcaaagc cgcccatcag cgcctggcgc acacgtaccg
    74461 ggtccgcacc ggcttttgaa gcaaatagca gggcttcaga aaccgcttca atattgagcg
    74521 ccacaataat ctgatttgcc actttgcagg tttggccgtc gccgttaccg cccacgaggg
    74581 tgatattttt accgagcagt tcaaacagcg gtttaacacg ttcaaatacc gcttcatcac
    74641 cgccaaccat aatcgacaac gtcccttcac gcgcaccgat ttcaccgccg gagactggcg
    74701 catcgagata atcgccgccc agttcattca cctgacgagc gaaacgctta gtttcaatcg
    74761 gggaaatgga gctcatatca acaatggttt tgcccttcag cgaggctttg gtacaaccat
    74821 tttcaccgaa cagaacttct tcaacctgtg gtgtatctgg caccataata aaaatgatgt
    74881 ccgatgcttc cgttacctgg cgcgcagttt caacactgac ggcacccagt gacagtaatt
    74941 catcagcaac cggtccaatg gttgtgacat gcagttgatg accggcacgc gccagattaa
    75001 tggccatcgg tgtacccata atgcctaagc caataaatcc cagtttcata attaacctct
    75061 tttaaatttc gcttttcctg aattcagaca acacgatccc ctgccaactt agcggcctaa
    75121 aaaagcattg cctgaacagc gttacgtttt aacggtacgg atccatccag cgtaaaccgg
    75181 cttccgtggt ggtttggggt ttatattcac acccaaccca accgttgtag tcagaatttt
    75241 cgattacctt aaagagataa tcataattaa tttctccggt ccccggttcg ccgcgatgcg
    75301 gattatcagc aatttgcagg tggccaattt tatcggccca ctgagtcatg gtgttggtta
    75361 attcaccttc catccgctgc atgtgataaa tgtcatactg aattttcaaa ttgcagcaac
    75421 caacatcatc aatcaatttc agcgcctgcc gagttccggt gagatggaaa ccaggaatat
    75481 caaaatggtt aataggttca atcagtaata aaatatcttc tttcatcagc atattcgcgg
    75541 cataacgcag gttttctaca agcgttgcgt gaatctgttc actgctgaaa ccagccggcg
    75601 ttttaccgac cagacagtta atttttttat tacccagcgc acgggcataa cgaatcgctg
    75661 ctgctacgcc atcccgaaac tcttcttcac ggccaggaat acaggcaata ccgcgctccc
    75721 ccgccgccca gtcacccgcc ggtaaattgt gcagcgtatg ttcgagttta ttactcgcca
    75781 gcacctgttt taattcttca atgtcgtagt cataaggaaa cataaattca acgccgcgaa
    75841 aaccacactg cgcagctttc tcaaaacggg cgagaaaatc atattctcca aataacatcg
    75901 ataaattagc agagaaacgt aacattattt atctccctta ttcatagtgc atgaagcagg
    75961 tttcagtcgg tgcgtccgct gcgttatcgg cgatatcttc aaattccatg acgttatcca
    76021 gttcgctgcc catcgaaata ttggtcacac gctcgagaat aacttccacg actaccggta
    76081 cccgatattg cgccattaag gctttcgcct gttcaaaggc tggcgcaata tcttccggtt
    76141 tgaagacgcg aatcgcttta caacctaaac cttccgctac ttttacgtgg tcaacaccgt
    76201 agccattcac ttcactggag ttgatattct cgaaagcgag ttgcacgcag tagtccatat
    76261 caaacgcgcg ctgcgactgg cgaatcagcc ccagataagc gttattgacc agtacatgga
    76321 tgtacggaat gttgaactgt gcgccaacag ctaactcttc aatcaggaac tggaagtcaa
    76381 agtcgccaga aatcgccacc acattgcgtt tcggatcagc ggcacaaacc cctagcgcag
    76441 ccggaatcgt ccagcctaac ggaccagcct gaccacagtt gatccagtgg cggtctttaa
    76501 agacatgcag catttgtgcc gcagcgattt gtgacagacc aatggtggtg acatagcaaa
    76561 catcgcgacc aaaggctttg ttcatctctt catacacgcg ctgcggtttc accggcacgt
    76621 tgtcgaaatg ggttttgcgc agcaaagtgc gtttgcgctg ctggcagtcg gcgacccatt
    76681 ctttacgaca cggcagacga cccgcttttt gcatctcctg cgccacttca accagcagtg
    76741 tcagcgccgc tttagcatca gagacaatgc cgagatccgg acacagcacg cgaccaattt
    76801 gcgtcggctc aatatcgata tgaacgattt tgcgcccttc ggtgtatttc tctaccgaac
    76861 cggtatgacg gttagcaaaa cggttaccga taccaaacac catgtcggac gccagcaacg
    76921 ttgcgttacc gtaacgatgc gcggtttgca gaccaaccat cccggccatc agttcatgat
    76981 cgtccgggat acagccccag cccat
//
LOCUS       DAFATA010000009        76214 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFATA010000009 DAFATA010000000
VERSION     DAFATA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.009"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..870
                     /locus_tag="LGJ20_000918"
     CDS             <1..870
                     /locus_tag="LGJ20_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK2385034.1"
                     /translation="SDRITVLRNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAG
                     RTLLSDKPVAAFKNYGKKGTIAPFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPAD
                     SGTALIKGKPQTLRSPHQASVLGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLR
                     PISRKEQQEIAERFIRQLGIRTPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEP
                     TRGIDVGAHAEIIRLIETLCADGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLA
                     ELSVPAIMNAIAA"
     gene            884..1906
                     /locus_tag="LGJ20_000919"
     CDS             884..1906
                     /locus_tag="LGJ20_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2385035.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            1893..2888
                     /gene="yjfF"
                     /locus_tag="LGJ20_000920"
     CDS             1893..2888
                     /gene="yjfF"
                     /locus_tag="LGJ20_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBK2385036.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(2921..3919)
                     /gene="fbp"
                     /locus_tag="LGJ20_000921"
     CDS             complement(2921..3919)
                     /gene="fbp"
                     /locus_tag="LGJ20_000921"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBK2385037.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            4095..5468
                     /gene="mpl"
                     /locus_tag="LGJ20_000922"
     CDS             4095..5468
                     /gene="mpl"
                     /locus_tag="LGJ20_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBK2385038.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(5551..6102)
                     /locus_tag="LGJ20_000923"
     CDS             complement(5551..6102)
                     /locus_tag="LGJ20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBK2385039.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            6196..7548
                     /gene="pmbA"
                     /locus_tag="LGJ20_000924"
     CDS             6196..7548
                     /gene="pmbA"
                     /locus_tag="LGJ20_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBK2385040.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            7731..8117
                     /gene="cybC"
                     /locus_tag="LGJ20_000925"
     CDS             7731..8117
                     /gene="cybC"
                     /locus_tag="LGJ20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBK2385041.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(8162..8626)
                     /gene="nrdG"
                     /locus_tag="LGJ20_000926"
     CDS             complement(8162..8626)
                     /gene="nrdG"
                     /locus_tag="LGJ20_000926"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBK2385042.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(8784..10922)
                     /gene="nrdD"
                     /locus_tag="LGJ20_000927"
     CDS             complement(8784..10922)
                     /gene="nrdD"
                     /locus_tag="LGJ20_000927"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK2385043.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(11316..12971)
                     /gene="treC"
                     /locus_tag="LGJ20_000928"
     CDS             complement(11316..12971)
                     /gene="treC"
                     /locus_tag="LGJ20_000928"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK2385044.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(13021..14442)
                     /gene="treB"
                     /locus_tag="LGJ20_000929"
     CDS             complement(13021..14442)
                     /gene="treB"
                     /locus_tag="LGJ20_000929"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBK2385045.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(14561..15508)
                     /gene="treR"
                     /locus_tag="LGJ20_000930"
     CDS             complement(14561..15508)
                     /gene="treR"
                     /locus_tag="LGJ20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBK2385046.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            15693..15746
                     /gene="mgtL"
                     /locus_tag="LGJ20_000931"
     CDS             15693..15746
                     /gene="mgtL"
                     /locus_tag="LGJ20_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBK2385047.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            15887..18583
                     /gene="mgtA"
                     /locus_tag="LGJ20_000932"
     CDS             15887..18583
                     /gene="mgtA"
                     /locus_tag="LGJ20_000932"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK2385048.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(18628..19084)
                     /locus_tag="LGJ20_000933"
                     /pseudo
     CDS             complement(18628..19084)
                     /locus_tag="LGJ20_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19150..19536)
                     /gene="ridA"
                     /locus_tag="LGJ20_000934"
     CDS             complement(19150..19536)
                     /gene="ridA"
                     /locus_tag="LGJ20_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBK2385049.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(19609..20070)
                     /gene="pyrI"
                     /locus_tag="LGJ20_000935"
     CDS             complement(19609..20070)
                     /gene="pyrI"
                     /locus_tag="LGJ20_000935"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBK2385050.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(20083..21018)
                     /gene="pyrB"
                     /locus_tag="LGJ20_000936"
     CDS             complement(20083..21018)
                     /gene="pyrB"
                     /locus_tag="LGJ20_000936"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBK2385051.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(21022..21156)
                     /gene="pyrL"
                     /locus_tag="LGJ20_000937"
     CDS             complement(21022..21156)
                     /gene="pyrL"
                     /locus_tag="LGJ20_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBK2385052.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(21437..21832)
                     /locus_tag="LGJ20_000938"
     CDS             complement(21437..21832)
                     /locus_tag="LGJ20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBK2385053.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(21963..22676)
                     /gene="bdcA"
                     /locus_tag="LGJ20_000939"
     CDS             complement(21963..22676)
                     /gene="bdcA"
                     /locus_tag="LGJ20_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2385054.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            22747..23340
                     /locus_tag="LGJ20_000940"
     CDS             22747..23340
                     /locus_tag="LGJ20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK2385055.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            23485..23937
                     /gene="tabA"
                     /locus_tag="LGJ20_000941"
     CDS             23485..23937
                     /gene="tabA"
                     /locus_tag="LGJ20_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBK2385056.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            24060..25032
                     /locus_tag="LGJ20_000942"
                     /pseudo
     CDS             24060..25032
                     /locus_tag="LGJ20_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036524.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            25048..25401
                     /locus_tag="LGJ20_000943"
     CDS             25048..25401
                     /locus_tag="LGJ20_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385057.1"
                     /translation="MQYFGDSEKIKYACSVINHIDFSRDQSKDFSCDQSKIKIAETLF
                     FNLDKEHYKNSRKLQELIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFFEGFFHEAT"
     gene            25388..25618
                     /locus_tag="LGJ20_000944"
     CDS             25388..25618
                     /locus_tag="LGJ20_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385058.1"
                     /translation="MKQPDFAKWYFYQLLKDYEGEQLYLNELGYVYGNEEKTNEIVKN
                     NPGYVVKIFEEKMVNELKIRTRMMKILRKIYV"
     gene            complement(25719..26723)
                     /gene="argF"
                     /locus_tag="LGJ20_000945"
     CDS             complement(25719..26723)
                     /gene="argF"
                     /locus_tag="LGJ20_000945"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK2385059.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            26885..27301
                     /gene="rraB"
                     /locus_tag="LGJ20_000946"
     CDS             26885..27301
                     /gene="rraB"
                     /locus_tag="LGJ20_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBK2385060.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(27347..27849)
                     /locus_tag="LGJ20_000947"
                     /pseudo
     CDS             complement(27347..27849)
                     /locus_tag="LGJ20_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            28042..29238
                     /locus_tag="LGJ20_000948"
     CDS             28042..29238
                     /locus_tag="LGJ20_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBK2385061.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(29294..32149)
                     /gene="valS"
                     /locus_tag="LGJ20_000949"
     CDS             complement(29294..32149)
                     /gene="valS"
                     /locus_tag="LGJ20_000949"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK2385062.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(32149..32592)
                     /gene="holC"
                     /locus_tag="LGJ20_000950"
     CDS             complement(32149..32592)
                     /gene="holC"
                     /locus_tag="LGJ20_000950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBK2385063.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(32726..34237)
                     /gene="pepA"
                     /locus_tag="LGJ20_000951"
     CDS             complement(32726..34237)
                     /gene="pepA"
                     /locus_tag="LGJ20_000951"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBK2385064.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            34504..35604
                     /gene="lptF"
                     /locus_tag="LGJ20_000952"
     CDS             34504..35604
                     /gene="lptF"
                     /locus_tag="LGJ20_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBK2385065.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            35604..36686
                     /gene="lptG"
                     /locus_tag="LGJ20_000953"
     CDS             35604..36686
                     /gene="lptG"
                     /locus_tag="LGJ20_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBK2385066.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(36847..38349)
                     /locus_tag="LGJ20_000954"
     CDS             complement(36847..38349)
                     /locus_tag="LGJ20_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBK2385067.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANSVFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(38479..39498)
                     /gene="ahr"
                     /locus_tag="LGJ20_000955"
     CDS             complement(38479..39498)
                     /gene="ahr"
                     /locus_tag="LGJ20_000955"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBK2385068.1"
                     /translation="MSMIKCYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            39694..39778
                     /locus_tag="LGJ20_000956"
     tRNA            39694..39778
                     /locus_tag="LGJ20_000956"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39728..39730,aa:Leu,seq:caa)
     gene            39942..41210
                     /locus_tag="LGJ20_000957"
     CDS             39942..41210
                     /locus_tag="LGJ20_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBK2385069.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKVVQKEARAENSGAYTFEA
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MMQWWADYLDMNRNKYITPYDYSKYKEILK"
     gene            41317..42111
                     /locus_tag="LGJ20_000958"
     CDS             41317..42111
                     /locus_tag="LGJ20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HBK2385070.1"
                     /translation="MAELLREQEVKIALIEWLYKKGMLSDATIINEMVVANWSRRADL
                     AIANGSLQAFEIKSDFDSLKRLDGQLKIFKSSFEKVTIVCAPKFTSEVSKRVSSDVGV
                     VEFQSNNSSVRFKIIQKGRVCSRLNKFVYLGFLLKSELKDLLNQYGITFSNELHRDCL
                     EVLASKLSLNKIREFVITSIKNRYHATSNDFLSRLHGNQEISINDLALLSKAKRRNNS
                     LVKFEFKACSEKSLNEYHKVDVDSIVRKYGDDVSYIPEKVLKRVVG"
     gene            complement(42139..43206)
                     /locus_tag="LGJ20_000959"
     CDS             complement(42139..43206)
                     /locus_tag="LGJ20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HBK2385071.1"
                     /translation="MKVYFENYSYYPALRTRSAEMTGLNNLSYENKKKILPLISLGKW
                     PRSEEIQVSLDKSLEVMSNLPFILDVTKDNSHHCASSFELLSPENGFKNWIEFCSRND
                     NIIPVVQMPDSAKLRDISIQARVLEELKGSIAFRIRNLNTDINKTLTSLVSMNSPENA
                     IVFIDLGYIRGNVSAITAAAINSINQIRTEIPEAIISVLATSFPSSVTNFCRENGQSG
                     YIDVIERELHQNIGGSDVAIYGDHGSIHSVVYDNIIGRYVPRIDIALNDSWYFERRPG
                     MNKEGFIEAAKSILAEYPHYQREDSWGAAMIRNAAIGDIAGMGSPAKWIAVRVNLHLN
                     KQIELSEALQYGFDHDEEDLI"
     gene            complement(43209..43610)
                     /locus_tag="LGJ20_000960"
     CDS             complement(43209..43610)
                     /locus_tag="LGJ20_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein gop"
                     /protein_id="HBK2385072.1"
                     /translation="MLNHVYGLIGVVGTIITIATSFIRNQDISQWLLVCSGWLAALLI
                     GWFTHRTIKAISNNHTNVIKSNMEVIKSHNESNQNLMAENKELIKELARTSEQKEKME
                     SIAAYLATQNPQINAMPRTASRPENIDSEAN"
     gene            complement(44021..44593)
                     /locus_tag="LGJ20_000961"
     CDS             complement(44021..44593)
                     /locus_tag="LGJ20_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HBK2385073.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSVQDSDILTAIGFRPDVA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(44667..45167)
                     /locus_tag="LGJ20_000962"
     CDS             complement(44667..45167)
                     /locus_tag="LGJ20_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HBK2385074.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQKYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(45164..45898)
                     /locus_tag="LGJ20_000963"
     CDS             complement(45164..45898)
                     /locus_tag="LGJ20_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HBK2385075.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPDKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            46451..46717
                     /locus_tag="LGJ20_000964"
     CDS             46451..46717
                     /locus_tag="LGJ20_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBK2385076.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            46714..47313
                     /locus_tag="LGJ20_000965"
     CDS             46714..47313
                     /locus_tag="LGJ20_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBK2385077.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            47306..47593
                     /locus_tag="LGJ20_000966"
     CDS             47306..47593
                     /locus_tag="LGJ20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HBK2385078.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            47586..48041
                     /locus_tag="LGJ20_000967"
     CDS             47586..48041
                     /locus_tag="LGJ20_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385079.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPETTPDNSYRNYL"
     gene            48177..48497
                     /locus_tag="LGJ20_000968"
     CDS             48177..48497
                     /locus_tag="LGJ20_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HBK2385080.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            48512..50845
                     /locus_tag="LGJ20_000969"
     CDS             48512..50845
                     /locus_tag="LGJ20_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK2385081.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLIAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHILEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDTDPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            51613..51840
                     /locus_tag="LGJ20_000970"
                     /pseudo
     CDS             51613..51840
                     /locus_tag="LGJ20_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012304637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            52116..52301
                     /locus_tag="LGJ20_000971"
     CDS             52116..52301
                     /locus_tag="LGJ20_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385082.1"
                     /translation="MNEKKYQSLRKKSISKGGMACIVMLIHTLSFSSLQMDLDVRVGD
                     VNWKHSIVLKTTGGKGE"
     gene            52318..52920
                     /locus_tag="LGJ20_000972"
     CDS             52318..52920
                     /locus_tag="LGJ20_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK2385083.1"
                     /translation="MYKIVTLESVAEIRLGMPFKSAIQDAGEQGSCYLIQTKDIGLDG
                     ILDLGTLTSVIPEGNPEKHYLFPNDILLRLRGPVFSAGIIEGNLGKPIITSNQLAVIR
                     CNENLILPHYLHWYINSTSGSKYIRSLSEGTSISKINSKTVSKMAVKLPNLRVQEKIG
                     FINRNWIKQKVIYNSLIQNGDVLFDGICENIIDSEELENE"
     gene            52913..54394
                     /locus_tag="LGJ20_000973"
     CDS             52913..54394
                     /locus_tag="LGJ20_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBK2385084.1"
                     /translation="MSEMKALHKSLLLACDLFRGKVDSSSYKDYIFSMLLLKYISDIK
                     ADYLSDLINEDYSELLEIVSRVPEDASFYKIYHEAKNHGDYIGERIDNSLRLIDQAID
                     SVCQNRGGYLFESIQFATVNFGARSERDRMLNNLLAIFARPEMNFGYTHDGNDKIKVA
                     CRYLFERIASDSAMRGAEFYTPEGISLLFAELLQPKGGDSICDPTCGSGSLLITLGEK
                     IKKSFKSNNYTLFGQEVNRSSWALAKMNMIMHNEINSRIEWGDIISSPQFLDTDNRLM
                     KFDVVASNPPFNIIGWNHDDLVHNDYGRFNLGFPPQAKGDYAFILHMVSTLKSATGRM
                     AILVSHGVLFRGGQEENIRRNLVSEGLLDAVIGLPDRLLFGTGIPTAILIFRKDKKNS
                     NVVFIDASDLAKPVKGRNLITDEIIRKVSECYFERSSVCNFSHVASFDEIQKNDFNLN
                     ISRYVKKHEEPAFVDLKGLRQQREELLSTLYELEREMKGFIDN"
     gene            complement(54440..54923)
                     /locus_tag="LGJ20_000974"
                     /pseudo
     CDS             complement(54440..54923)
                     /locus_tag="LGJ20_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131862.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
     gene            54988..55170
                     /locus_tag="LGJ20_000975"
     CDS             54988..55170
                     /locus_tag="LGJ20_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385085.1"
                     /translation="MGAFLLGSDIATMSEHSSLEGLLSLDKERLMDVLVRHYDVGDAG
                     IEQIINDFWDGMVSDR"
     gene            55643..56665
                     /locus_tag="LGJ20_000976"
     CDS             55643..56665
                     /locus_tag="LGJ20_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385086.1"
                     /translation="MTEYWWKDLFDRNNNWQGLEFTIRAHQGVAQAMDMLSGHHGRMA
                     LQLHGETLFWASMLKDHSGVWLVFNVDHSGRQSLVPPVTSADVEQIKCKGAEVWTGEW
                     CRYFTRQLMNTPVPLLPPRRWLLRPVDTTRPCNQSISIENWRFDSPESTGNIGCDWTL
                     YGEDIPDLTNPEKIRLVDWWWGGNLLLGRYHVHFNAGRLKWWRKKCREETLPPILVWY
                     IAGLGSFVILDGHYRLQAAIEEGTPPSFLVLSELNERNFPVDQEHQARITRALERQSQ
                     NPGCSIDGVNQTLINLYDNRYLYASTYSRAILGKGESWAADVNSYLHKHNLEEYLDKI
                     VNRVND"
     gene            56687..57229
                     /locus_tag="LGJ20_000977"
     CDS             56687..57229
                     /locus_tag="LGJ20_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385087.1"
                     /translation="MKDFFREYRDTILASVGIIVAVFSLYFTQEQSKEQIKHNHISVE
                     PRINAYVSNDGRINKSGLYIINNGMGTAFVSNITVMVDGKKIDPVGDNIFLASVHALG
                     LDITCFVIGGPRPGDSFRVGDEVFLVEARGTDSRCDVDSLELRLAASQTGRLDFILDV
                     ESIYGDKFRYIYSQNKQVKL"
     gene            57249..57433
                     /locus_tag="LGJ20_000978"
                     /pseudo
     CDS             57249..57433
                     /locus_tag="LGJ20_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683738.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(57497..58585)
                     /locus_tag="LGJ20_000979"
     CDS             complement(57497..58585)
                     /locus_tag="LGJ20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385088.1"
                     /translation="MAQERSVIKFIRPCLSIHEDKKILTSEYCWQSLNKKRAVQINLD
                     NCSLQRMEELSTNSTLEDVRRVGLLPLVELLQSGTVLLTAIGVNEMPDIWVEKSMAAY
                     QNFCHLFWPGHIDDPEATFRDYNPDSENPKVDFKKLTDEARTVYGMHYISMLQIQNVK
                     LNYSHLSPEDKFETYLYSMISFIDLISAYDLEIAKYAFWDLDANTINQLPENIHKRRK
                     YIKQNFYKNGSNIDKCRWNAFDAAMDLHWLTGANFSEDIGATIDLNGTKFNIEHWVGT
                     TDHKLHFIAQDIHHVYHDGSTMKALHSIRESEMSKFLYWRNVDNIAESVLRFRSHLNL
                     NVDNNIAKRIDMAVEHIEHELMNYFIQH"
     gene            complement(58619..60703)
                     /gene="brxL"
                     /locus_tag="LGJ20_000980"
     CDS             complement(58619..60703)
                     /gene="brxL"
                     /locus_tag="LGJ20_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HBK2385089.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPAAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            complement(60714..63311)
                     /gene="pglZ"
                     /locus_tag="LGJ20_000981"
     CDS             complement(60714..63311)
                     /gene="pglZ"
                     /locus_tag="LGJ20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HBK2385090.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLELENVTLI
                     NMTHASQLSVKKRIEIDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDEASLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKRHCLDSVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADPQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKDAYDYCAQQ
                     MQAALRPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEVVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVADTAGVSDNSEFLIPKGIQRFHFSGGARFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNSTMEKRVREVTLKLIGANFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            complement(63488..67153)
                     /gene="pglX"
                     /locus_tag="LGJ20_000982"
     CDS             complement(63488..67153)
                     /gene="pglX"
                     /locus_tag="LGJ20_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HBK2385091.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLEHGFRMLSHPETPTAFEVLDHVPEVAEALLPESKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHAMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDDIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNILSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENITLAQTDSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQEGDFQQKAAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGSKGMNGELKEFAKNNFPDSKSDLFAMFIERGFLWLKNAGFNSMVTMQSWMFL
                     SSFENMRENILTNYTIETMVHMGNGVMKIAFGTNATVFRNNHISTYQGSFSYVENGNI
                     NQEGNPKQFPVINERLKTATTNDFKKIPGSPIAYWISHSLLNVFETSKPISEYFDTRL
                     GMSTANNDEFLRFWHEVSIQNIGFGCKDREEASNSLKKWFPYAKGGSYRKWYGNHDYV
                     VNWENDGEKIRNFGIENGKVRSHNYNLDFIFRKGITWSDVTSGSNAFRVLPQGFLFDG
                     RGSSGFCEDNLRPTILCLLNSKLSYNIINIINPTIAINVGEIAKIPLHNNLMNNKNNI
                     ENLTLEIIDLHKKDWDEKENSWNFYRNALIDKKSNNLKEALLRKYANDTIEINRCHSI
                     ESELNHQINITYDLESYVNQTVDLAQITLFKNMAYIFSDTANKESLYVTILISELISY
                     SIGCQMGRYSLDREGLVYAHEGNKGFSELVAEGAYKTFPADSDGILPLMDDEWFEDDV
                     TSRVKEFVRTVWGEEHLQENLEFIAESLCLYAIKPKKGESALETIRRYLSTQFWKDHL
                     KMYKKRPIYWLFSSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANIDRLND
                     QLDEASGGEATRLKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVNYGKFG
                     DLLADVKAITGNAPEVI"
     gene            complement(67199..70840)
                     /gene="brxC"
                     /locus_tag="LGJ20_000983"
     CDS             complement(67199..70840)
                     /gene="brxC"
                     /locus_tag="LGJ20_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HBK2385092.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLEASRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADIDAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKGDILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWISMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKSKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELRKKQEAQLRAANEAKRAAES
                     EQTYVVVEKPVIQPVPKKTHLVNVASEMRNATGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            complement(70852..71454)
                     /locus_tag="LGJ20_000984"
     CDS             complement(70852..71454)
                     /locus_tag="LGJ20_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HBK2385093.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIINMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            complement(71451..72053)
                     /locus_tag="LGJ20_000985"
     CDS             complement(71451..72053)
                     /locus_tag="LGJ20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBK2385094.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALTLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNNQVRQHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            72350..73348
                     /locus_tag="LGJ20_000986"
     CDS             72350..73348
                     /locus_tag="LGJ20_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385095.1"
                     /translation="MALYNIANKELHALEKTTFTLEGLQERYDLQEAIKKNIDIIAPD
                     CLVISEEFSDWEDSRRRIDLLAIDKQANLVVIELKCDETGAHMELQALRYAAMISTMS
                     FAKACEYFQTYLKKQNCDADAKEKILEFVELDETELVDFGKDIRIVLASSDFSKELTT
                     TAIWLRDKGVDIRCVRLTPYRFNDDVLINAEQIIPVPELEEYQVKFREKRDEQLISSQ
                     KKEKDYTWYIYKDKELNKRKLALELLRDWIRQFNPASYNDLINGLSEDFKKRTVMLVD
                     QIPEKQKSRYHINEDALITLPSGEIVAISNQWGIVNIELLIEFVRQNGFVVEKAEQ"
     gene            complement(73459..74331)
                     /locus_tag="LGJ20_000987"
     CDS             complement(73459..74331)
                     /locus_tag="LGJ20_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK2385096.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVEKLFWDFDGKTIALPQVRKNYPF
                     EGFVEWHRKEVFRG"
     gene            74413..74535
                     /locus_tag="LGJ20_000988"
     CDS             74413..74535
                     /locus_tag="LGJ20_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK2385097.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            complement(74543..75523)
                     /locus_tag="LGJ20_000989"
     CDS             complement(74543..75523)
                     /locus_tag="LGJ20_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBK2385098.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(75588..>76214)
                     /gene="nanM"
                     /locus_tag="LGJ20_000990"
     CDS             complement(75588..>76214)
                     /gene="nanM"
                     /locus_tag="LGJ20_000990"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBK2385099.1"
                     /translation="AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGE
                     SPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGV
                     AGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSG
                     ELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
BASE COUNT        18700 a        18384 c        18928 g        20202 t
ORIGIN      
        1 agcgatcgga tcaccgtctt acgcaacggc agtttcgtag gctgtcggga aacgcgcgag
       61 ctaccgcaga tcgaactggt aaaaatgatg ctggggcgcg agctggatac ccacgcgcta
      121 cagcgtgccg ggcgaacatt gttgagcgac aaacccgttg ccgcgttcaa aaattacggc
      181 aaaaaaggaa cgatcgcacc gtttgatctc gaagtgcgcc ccggcgagat cgtcggcctg
      241 gcaggcttgc tgggatcagg acgtaccgaa accgccgaag tgatcttcgg tatcaaacct
      301 gctgacagcg gcacggcgtt gatcaaaggc aaaccgcaaa ccctgcgatc gccacatcag
      361 gcttcggtac tgggcatcgg cttctgcccg gaagacagga aaaccgatgg catcatcgct
      421 gccgcctcgg tgcgggaaaa tatcatcctc gctctccagg cccagcgcgg ctggctacga
      481 cccatttccc gtaaagaaca gcaagaaatt gccgaacgct ttatccgcca gcttggcatt
      541 cgcacacctt caactgaaca accgattgaa tttctctccg gcggcaatca gcaaaaagta
      601 ttgctttcac gttggctact gactcgtccg caatttctga tcctcgatga gccaacccgt
      661 ggcattgatg ttggtgccca cgccgagatc atccgcctga ttgaaacgct atgcgccgat
      721 ggtctggcgc tgctggtgat ctcctccgaa ctggaagaac tggtgggcta tgccgaccgg
      781 gtgatcatca tgcgcgatcg caaacaggtg gcggagatcc cgctggcaga gctttccgtt
      841 ccggcgatca tgaacgccat tgcggcgtaa ggagaacagt gtgatgcctc aatctctccc
      901 ggacactacg ccgccgaaaa ggcgctttcg ctggccaacg ggaatgtcgc agctggtagc
      961 actattgctg gtgctgctgg tcgacagcct ggtagccccg catttctggc aggtagtgct
     1021 tcaggatggg cgtttgttcg gtagccccat agacattctt aaccgtgcag cacccgttgc
     1081 gttactggcg attggtatga cgctggtgat cgccaccggt gggattgatc tctccgtagg
     1141 ggcggtgatg gctatcgccg gagccacaac ggctgcgatg acggtcgcgg gattcagcct
     1201 gccgattgtt ttgttaagcg ccctgggcac cggcatcctg gcgggattgt ggaacggcat
     1261 actggtagcg atcctcaaaa ttcagccgtt tgtcgccacc ctgatcctga tggtcgccgg
     1321 gcgcggcgtg gcgcaactga tcaccgccgg gcagatcgtc acgtttaact cgccggatct
     1381 ctcatggttt ggcagcggat cgctgttgtt cctgccaacg ccggtcatta tcgcggtgct
     1441 gacgcttctc ctgttctggc tgttgacccg caaaacggcg ctggggatgt ttatcgaagc
     1501 cgttggtatc aacattcggg cggcaaaaaa tgccggggta aacacgcgga tcatcgtcat
     1561 gctcacctac gtgttgagcg ggctgtgtgc ggcgattgcg ggcattatcg tggcggcgga
     1621 tattcgcggt gccgatgcca ataacgccgg gttatggctg gagctggacg ccattcttgc
     1681 ggtggtgatt ggcggcggat cgctgatggg cgggcgcttt aacctgctgc tctcagtcgt
     1741 gggggcgctg attattcagg ggatgaacac cggaatttta ctttctggct ttccgccgga
     1801 gatgaaccag gtcgtaaaag cggtggtggt gctttgtgtg ctgattgttc agtcgcaacg
     1861 ctttatcagt ctgattaaag gagtacgtag ccatgataaa acgtaatttg ccactgatga
     1921 tcaccatcgg cgtctttgtg ctgggctatt tctactgcct gacgcagttt cccggttttg
     1981 cttccacaag agtgatctgc aatatcctga ccgataacgc ctttcttggg ataatcgccg
     2041 ttggcatgac ctttgtgatc ctctctggcg gtatcgatct ctccgtcggt tcggtgatcg
     2101 cctttactgg cgtgtttctg gcaaaagtgg ttggcgattt cggcctctcg ccgctgctgg
     2161 cgtttccgct agtgctggtg atgggctgtg ccttcggcgc atttatgggg cttctgatcg
     2221 acgccctgaa gatcccggca tttatcatta cgcttgcggg gatgttcttt ttgcgcggcg
     2281 tcagctatct cgtttcggaa gagtcgattc cgataaacca tcccatttat gacacgctct
     2341 caagccttgc gtggaaaatc cccggcggcg gtcgccttag tgcgatgggg ctgttgatgc
     2401 tggcggtggt ggttatcggt attttccttg cgcatcgtac ccgttttggc aatcaggtat
     2461 acgccattgg cggcaacgca acgtcggcaa acctgatggg gatttctacc cgcagcacca
     2521 ctattcgcat ttatatgctc tccaccggac tggcaacgct ggcggggatt gtcttctcga
     2581 tttataccca ggccggatat gcgctggcgg gcgtaggtgt ggaactggat gctatcgcct
     2641 cagtggtaat tggcggtacg cttttgagcg gtggcgttgg aacggtatta gggacgcttt
     2701 ttggcgtggc gattcagggg ctaattcaga cttatatcaa ctttgacggt acgctgagtt
     2761 cctggtggac gaaaatcgcc atcggcattt tgctgttcat ttttattgcc ttacagcgtg
     2821 gattaacggt gctgtgggag aatcgtcaga gttcgccagt gacaagagtc aatattgcgc
     2881 agcgataaaa acgcctctcc gtgtggagag gcgcaggaga ttacgcgtcc gggaattcac
     2941 ggataaagcg ttcgacatct tcaaccatat ggtcgttgcc gacaaagaac gaacggcgct
     3001 ggtgcagagt ttccgggatg atatccagaa tacgctcttt gccatcgctc gctttaccgc
     3061 ccgcttgttc cgccaggaat gccatcgggt tgcactcata cagcaaacgc agtttgccgt
     3121 ccgggtggct ggcggtgctt gggtagagat aaataccgcc tttcagcagg ttacggtgga
     3181 aatccgcgac cagtgaaccg atataacgtg aggtatacgg gcggttggtg gatttatctt
     3241 cttcctggca gaatttaatg tacttcttca ccccgttcgg aaacttaatg tagtttcctt
     3301 cgttgatgga gtaggttttg cctttctccg ggaagcgcat ccgttcctgg cacaggcaga
     3361 aaacgcccag cgaaggatcg taagtaaagg cgtgaacacc gcatccggtg gtgtaaacca
     3421 gcatggtaga ggagccgtat accacgtaac ctgccgcaac ctgtttgtta ccaggctgga
     3481 ggaaatcttc ttccgttacc ggcgtgccaa caggcgtaac gcggcggtag atggagaaaa
     3541 tggtaccgac agagacgtta acatcgatgt tggacgagcc atccaggggg tccatcagca
     3601 ccacgtattt tgcgtgttca cagccttcaa agacgacaat ctcatcttct tcttcagagg
     3661 caatgcccgc aacgatatcg cgtgctttca gtgcggcttt cagtttttca ttagcgaaca
     3721 agtcgagttt ctgctgaacc tcgccctgca cgttctcagc accgctggca cccaggatat
     3781 caaccagtcc tgctttgttg atatcgcgat ggataatctt ggcgcccagt tttattgccg
     3841 acagcaaagc agtgagctca ccggtagcat gagaaaactc gtgctgcttt tcgacaataa
     3901 attcacctaa cgttttcata aaactttccc tgcaatgttt atggagtaaa gcgaccgcaa
     3961 caatcttaac aaataatctc aatgttgcgc tcaggtgaat cgcgccagca aattgcggat
     4021 tatcctgaaa tgcgtttctc acttgcccga catatgcgta aaatgagcgg cagattaaaa
     4081 aaggatagtg acgtatgcgc attcatattt taggaatttg tggcacattt atgggcggtc
     4141 tggcgatgct ggcgcgccag ttaggccatg aagtaacggg ttcggacgcc aatgtgtatc
     4201 cgccgatgag caccttactt gagaagcaag gcattgagct gattcagggt tacgatgcca
     4261 gccagctcga tccgcagccg gatctggtga ttattggcaa cgccatgacc cgtggaaatc
     4321 cgtgtgtgga agcggtactg gaaaaaaaca tcccttatat gtcaggtcca cagtggctgc
     4381 acgattttgt gctgcgcgac cgctgggtgc tggccgttgc cggtacacac ggcaaaacta
     4441 ccaccgcggg aatggcgacc tggattctgg aacagtgcgg ttacaaaccg ggatttgtga
     4501 tcggcggtgt gccggggaac tttgaggttt cggcgcgtct gggcgaaagc gacttctttg
     4561 ttatcgaagc ggatgagtat gactgcgcct tcttcgacaa acgctctaaa tttgtccatt
     4621 actgcccgcg tacgctgatc ctcaacaacc ttgagttcga tcacgccgat atctttgacg
     4681 acctgaaagc gatccagaaa cagttccacc atctggtgcg tatcgttccg gggcagggcc
     4741 gtattatctg gccggaaaat gacatcaacc tgaaacagac catggcgatg ggctgctgga
     4801 gcgagcagga gctggtgggt gagcagggtc actggcaggc gaaaaagctg accactgacg
     4861 cttctgaatg ggaagtcttg ctggatggcg aaaaagtggg cgaagtgaaa tggtcgctgg
     4921 tgggcgaaca taatatgcac aatggcctga tggcgattgc ggcggctcgc catgttggtg
     4981 tagcgcctgc agatgccgct aacgcgctgg gttcgtttat taacgctcgt cgccgtctgg
     5041 agttgcgtgg tgaagcgaat ggtgtgacgg tatatgacga ttttgctcat cacccgacgg
     5101 cgattctggc aacgcttgcg gcgctgcgcg gcaaagttgg cggtacggcg cgcattattg
     5161 ctgtgctgga gccgcgctcg aataccatga aaatggggat ctgcaaagac gatctggcac
     5221 cttcattagg tcgtgccgat gaagtcttcc tgctgcaacc ggcgcatatt ccgtggcagg
     5281 tggcagaagt ggcagaagcc tgcgttcagc ctgcacactg gagtggcgat gtggatacac
     5341 tggcagatat ggtggtgaaa accgctcagc ctggcgacca tattctggtg atgagcaacg
     5401 gcggttttgg tgggatccat cagaaactgc tggatggtct ggcgaagaaa gcggaagccg
     5461 cgcagtaatt cggccttagc ctgagatagc attgccggat aaggcgttta cgccgcatcc
     5521 ggcaaaccat actcacatca acaacgaaaa ttacccttca ttctccgcca gttcgcgcag
     5581 atactggaaa atctggcgtg cggatttcgg cggcttattc ccttctttct ctttcttcgc
     5641 gttacggatc agagtacgca gctgctgacg atcggcatcg ggccacagat tcaatacttc
     5701 agcgatggcg tcatcacctt gatcgatcag acgatcgcgc aggttttcca gtttatggaa
     5761 cagtaccacc tgctggttgt gacggttttt cagcttatcc agcgcctgac gaataggctc
     5821 tacgtcgcgc tggcgcagca tcttaccgat gagctgcaac tggcggcggc gaccttccat
     5881 cttaatacgc tgggcgagct caatagccgc gcgcagatcc gcatctaacg ggatcttatc
     5941 cagcgcgttt ttccccagat caacaatttc cgcgccaagg cgttttagct cctcggcatc
     6001 acgtttaatt tcacttttac tgacccagat aatttcatcg tcttcgtctt cgatgtcatc
     6061 accgggaacg tcgtcgagcc agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa
     6121 agaggctaat gttaccagtt aagatgcgca ctgaaaaacg gttctctgtt agacttcaga
     6181 gaaactctct acattatggc acttgcaatg aaagtaatct ctcaagttga agcgcagcgc
     6241 aagattctgg aagaagcagt ttccactgcg ctggagttgg cctcaggcaa atcggacggt
     6301 gcggaagttg ccgtcagcaa gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg
     6361 gagaatgttg aattcaatag cgatggcgcg ctggggatca ctgtttatca ccagaaccgc
     6421 aaaggtagcg catcatccac cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg
     6481 gcgctggata ttgcccgtta tacctcgcca gatccctgtg ccggcgtggc agacaaagag
     6541 ctgctggcct ttgacgcacc agatctcgac ttgttccacc ctgcggaagt ttccccggat
     6601 gaagccattg aactggcggc ccgcgcagaa caggcagcat tacaggcgga taaacgtatc
     6661 accaataccg aaggtggcag ctttaacagc cactacggtg tcaaagtttt tggcaacagc
     6721 cacggcatgt tgcagggtta ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc
     6781 gaagaaaatg gcgatatgga gcgtgattac gcctacacca ttggccgcgc gatgagcgat
     6841 ctgcaaacgc cagagtgggt cggggccgac tgtgctcgcc gtactttatc gcgcctgtca
     6901 ccgcgtaaac tctccaccat gaaagcgcca gtcatttttg ccaatgaagt ggcaaccggg
     6961 ctttttggcc atctggtggg ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt
     7021 ctgctggatt cgctgggtaa acaaattctg ccggactggc tgaccattga agagcatccg
     7081 catctgctga aagggctggc gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt
     7141 cgcgatatta ttaaagatgg catcctgact cagtggctgc tgaccagcta ctcggcgcgg
     7201 aaactggggc tgaaaagcac cggacatgcg ggcggtattc acaactggcg gattgccgga
     7261 caagggctaa gcttcgaaca aatgctcaaa gagatgggca ccgggctggt ggtgacggag
     7321 ctgatgggcc agggcgtgag tgccattacc ggtgattatt cccgtggtgc agcgggcttc
     7381 tgggtagaga atggcgaaat tcagtatccc gttagtgaaa tcaccatcgc aggtaattta
     7441 aaagatatgt ggcgcaatat tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata
     7501 cagtgtggtt ctgtgctgtt gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg
     7561 acctgccgcg ctataaacta aaaaattaca caaaatcatt cgcactgcat cgagacggaa
     7621 agtgaatgag ggccggggag cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg
     7681 tcgatgaggc agtgtgaagg atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa
     7741 gcctgttagc tattcttgca gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg
     7801 atcttgaaga caatatggaa accctcaacg acaatttaaa agtgatcgaa aaagcggata
     7861 acgcggcgca agtcaaagac gcgttaacga tgatgcgcgc cgcagcgctg gatgcgcaaa
     7921 aagcaacgcc gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc
     7981 gccacggttt cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag
     8041 gtaaagtaaa agaagcgcag gctgctgcag agcaactgaa aacgacccgc aacgcctatc
     8101 accagaagta tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt
     8161 atcatcgcaa atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt
     8221 ttaaatcctg cacaaatttg ccgtcgacca gcacgttaat caaatcaaca acctgcattt
     8281 gcgcagcgtt gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac
     8341 actcggcgcg gatgcgtttt accagtttca gaatatccgg cacgttttgc ggatgcagcg
     8401 gatcgccgcc ggagagggaa atcccctggc gtttgatacg agtgtcattc agatctttaa
     8461 tgatcttgtc ttccattgct ttggtaaatg gctgaccaga atttacccgc catgtgcttt
     8521 tgttatagca accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc
     8581 cggggccgtt gacgatgtcg acaggatagt actgatgata attcattatt tcgcctggct
     8641 tatcagtccg cgtgtaggct gcatccggta atcaatgcct gatgcgacgc tgtcgcgtct
     8701 tatcaggcct acagccgttg ccaaatgtag gccggataag gcgtttacgc cgcatccagc
     8761 atttgcttag cgcagagtga agattaacct atctgcccat tccccaaatg tttaacgcgg
     8821 cgcttaactt cttcctgctt accagcgtta aacggacgtg catccgggct acctaaatat
     8881 ccgcacacgc ggcgagttac cgacacacgg gaggcgtcat ggttaccaca tttcgggcaa
     8941 gtgaagcctt tgctggtgca ctcgaactca ccggtaaagc cacactcgta gcactcatca
     9001 atcggtgtat tggtgccgta atacggtaca tgctgatagc tgtaatccca gacatcttcc
     9061 agcgccttca ggttgtgctg aatgtttgga tactcgccgt agcaaatgaa accaccgttc
     9121 gccagcggcg ggtaaggcgc ttcaaagtcg atcttgtcgt acgggttcac cttcttctcc
     9181 acatcgaggt ggaaactgtt ggtgtagtaa cctttatcgg tcacgcccgg caccacgcca
     9241 aactcagcag tatcgagacg gcagaagcgg tcgcacaggt tttcactcgg cgtgctgtag
     9301 agactgaaac cataacccgt ttcttctttc cactgatcca ctgcctgacg cagacgttca
     9361 acaatggcga tacctttcgc gcgaagctgc tcgttgtcgt aaacatgctc gccgccgaac
     9421 agcgcgttaa tggtttcgtg gatgccgatg taacccagcg aaatagacgc acgaccgttt
     9481 ttgaagattt cagaaacatc atcgtcagca ttcagacgta cgccacaagc accttccata
     9541 tagaggatcg gggcgacgcg cgctttcacg ccttcgagac gagcgatacg ggtcatcagt
     9601 gccttacgtg ccagcaccag acgttcatcc agcagcttcc agaaggtggc ttcatcgcct
     9661 tttgcttcca gagcaatacg cggcaggttc aggctgatca cgccgaggtt gttacgacca
     9721 tcgtggatct gctcgccgtt ttcattttcc cacacgccga ggaagctgcg gcagcccatc
     9781 ggggttttaa acgaaccggt gactttcact acctgatcgt agttcaggat atccggatac
     9841 atgcgcttgc ttgcgcactc cagcgccagc tgtttgatgt cgtagttcgg atcgcctttt
     9901 ttatggttca gaccatcgcg aatcgcaaac accagtttcg ggaacaccgc agttttacgg
     9961 tttttaccga ggcctgcgat acggttacgc aggatcgatt cctgaatcag gcgcgattcc
    10021 cagctggtgc ccagaccaaa accaaaggtt acaaacggcg tctgaccgtt ggcggtgtgc
    10081 agggtgttta cttcgtactc cagcgactgg aaggcatcgt agcactcttt gatggtacga
    10141 gagtttgcgt agccttcggc gtccgggatg ttccactctt ctgcggtctt gcgatgtttg
    10201 ttgtagctgg cggtaacaaa cggcgccagc acttcatcga tacggttaat ggtggtgccg
    10261 ccataaatat ggctggcgac ctgagcaata atctgcgcag ttaccgcggt tgccgtagag
    10321 atcgacttcg gcggttcaat ctcggcgttc cccattttaa agccctgggt cagcatgcct
    10381 ttcaggtcga tcaacatgca gttgaacatc gggaagaacg gcgagtaatc gagatcgtga
    10441 tagtgaatat cgccacgctc atgtgcctgc accacgtcac gcggcagcag gtgctggcgt
    10501 gcatagtgtt tagccacgat cccggccagc aggtcgcgct gggttggaat caccttgctg
    10561 tctttgttgg cgttttcgtt gagtaacgag gcgttggtct gctcgaccag accacggatc
    10621 tcctggttca ggcgaccgcg tttttcacgt tcaatgtcgc gatcgtgacg gtactcgatg
    10681 taagcacgag ccagttgttt gtatggaccc gacatcagct ggttttcaac tgcggtctgg
    10741 atctcgttga tatccacctg gttgcggccc tgcatctgct cgctgacaac cgcggcaaca
    10801 gtggcgcaat aatcggcatc atcgacttcc gctgctttag ctgcacgcag aatcgcttct
    10861 ttgatgcgct ctgatttaaa cggcactttg cagccgtctc gtttcatcac atgcggtgtc
    10921 atgatcgctc catattttta agaacaggtt atccacagaa attgggaaag gcgttttccg
    10981 gttttttcgt ttcgttttcc ggtgctttcc gcaatcgcca tccgcttatc cacaacgccg
    11041 cacctttttc gtggggagct gtagtagcaa ttatagtcga ttaatacaac atattgggtt
    11101 gggacgcatt ttaaagtcta tatatagtgc tttgcatcaa ggatgtttga actttttttg
    11161 atgtagctca aagtaaaaag cagagcgtac ggatgacggg cgctacagcg ttatgtaaat
    11221 tttttaatga atttgctggt tgaaaaatca acaaaaacaa catgctgaca gacaaaaccc
    11281 cggaatgaca ggcattcccg gggcttagcg gatacttact tctgtaacca ccagacagcc
    11341 tcaaaaggcc gtaaagtcat ggcacagggt tgtggtgagg cttcttcgta gttatgcatc
    11401 acaagctgcc agttgccgcg catttgccct ggctgccagg gttggatctc acggctaagg
    11461 ttggcaatga ccagcaaggt ttgccccttc cattcacggc gatagcacca caatacaggg
    11521 ctgtttggca gcagatcctg gtaattgccc catgtcagga tggcttcttg cttacgcagt
    11581 gcgattaact tttggtaggt gtaaaacacc gaggagtcat cggccagcgc ggcttctacg
    11641 ttgatttgtt gatagttatc gcccaggcca atccacggtt cgccagccgt aaacccggca
    11701 ttatcgccgt tgctccattg catgggcgtg cggctgttgt cacgggattt actggcgagg
    11761 attgccaata actcgtcggc atcacgacca tcgttgcgca gctcggcaaa catattgagg
    11821 ctctccacgt cgcgatagtc agtaatgcgc gtgaaatgcg ggttggtcat gccaatctct
    11881 tcgccctggt agatatacgg cgttccctgc atgccatgca gcaccatcgc cagcattttt
    11941 gccgcaggca cgcggtattc accttcatca ccaaagcgag aaataatgcg cggctgatcg
    12001 tggttacacc agaacaaggc attccatgcc acgttgtgca ttccttgttg ccagtggcgg
    12061 aacaatgttt tcaacgccac aaagtcaggt ttagccagcg tccatttttc accaccggga
    12121 taatcgacct tcaggtgatg aaaattaaag gtcatcgaca attcactgcc tgtcagtgcc
    12181 gcgtatcgct ggcaatgctc aaggctggtg gaggacattt cacctacggt cattaacccg
    12241 cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg tggcccgtcg
    12301 gtgtaaaagc gacgcccgtc gccatccagg tcttcaggga aacgcgggtc tttggagatc
    12361 agattcacca catccaggcg caacccgtcg accccacgat cggcccagaa ttcacagact
    12421 tttttcagct ctgcgcgtac cgctggattc tcccagttga gatccgcctg ttctggtgca
    12481 aagagatgca aatagtactg ttcgctttcc gcatgccagc gccacgcact accgccaaat
    12541 tttgaacgcc agttgttcgg tggcgtttct ggttcgccat cgcgccagat ataaaactgg
    12601 cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg catgttgggt agaggtatgg
    12661 ttaaacacca tatcgagaat gatacgaatc ccgcgcgatt ttgcctgcgt caccagttcg
    12721 tcaaaatcgt ccagcgtgcc gtaggtggga tcaatcgccg tatagttcgc tacgtcgtaa
    12781 ccgttatcga cctggggaga gatataaaag ggggttagcc agatggcatc aacgcccagt
    12841 ttatgcagat agtccaggcg ttggataacg ccacgtaaat cgccggtgcc gctacccgtg
    12901 gtgtcctgaa aactctttgg ataaatctgg tagataacgc cgttttgcca ccagtgggga
    12961 agattagtca ttacgttatt cctgcaaatg cgagggggcg caattgcgcc ccgaagaaaa
    13021 ttaaacaata tccagcgtgc ccaggcggta tttccgctga tagataaacg aggtgagtac
    13081 aatcgggatg atgatggcga taaccattgc cagcgcaaat acctgccagt agctcggttg
    13141 aatcgagaga attcccggca ggccgcctac gccgatacca ttcgccataa cgccgttcag
    13201 gccgcatagt aatcctgcca gaccagaacc aatcatcgcg cacagcatcg ggaagcgata
    13261 tttcaggttg atgccgtaca ttgcaggctc agtgacccca agccaggcgg agatagcggc
    13321 aggcacggag atctcgcgtt cattgtgctt gcggctggaa atgataatgc ctatcacggc
    13381 ggagccctga gcgatattcg acagcgcaat cagcggccac actggcgtgc cacccatgct
    13441 ttgaatcatc tgcaaatcaa tagcaagggt ggtctggtgt acgccggtga tcaccagcgg
    13501 cgcgtacagg aagccaaaca atgccgcacc aatcggagca aagctgccgg tcatcaggtg
    13561 acgtaccgca aaggcaacgc catcgccaat catgcgacca aacggaccaa tcagcgcatg
    13621 ggcgaggaac accgcgagga tcagcgaaca gacgggcacc accaccagat agaggtaatc
    13681 cggtacgatg cgtttaaggc gagtttcaat aacgcccagc gccagcccgg ctaacagtgc
    13741 cgggatcacc tgcgcctgat agcccacttt ggcgatgctg aacatgccaa agtcccacac
    13801 ttccggcagc tgctgcccga gcagataagc gttcatcagc tggggggaaa ccagtgtcac
    13861 gccaagcacg ataccaagga tcggcgtgcc gcccattttt ttcaccgctg accagcaaat
    13921 cccgaccggc aggtagaaga agatcgcttc accgatcaac cacagaaaat cgtagatcgt
    13981 ttgcagggaa gggtacattt gcgccagcgt ttgaccgttg ctcatgggca aatcgccgat
    14041 cacattacga aaaccgagga tcaaaccgcc gctaatcaac gcgggcagca acgggaagaa
    14101 gatctccgcg aaatgagaga tcaactgctc atgccatttc atattctgcc gggcggcttt
    14161 ttttacctgc tctttatcaa cctgcgcctg tccggttgac gctattagtg cttgatagta
    14221 atcacccacg ttggtgccaa tcaccacctg aaattgcccg gcattggtga aacagccttt
    14281 caccatgggg agttgctcaa tttctttcgg tctggcattg gccggttggt tgaggacaaa
    14341 gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc cgaccagttc
    14401 aatcaaccga tcgatatccg tttggtttat tttgctcatc ataaagcccc atggcagatg
    14461 acatttttgg ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg gaacgttccc
    14521 gaaacgcagc gaagatcaca atttatcgtt caggaaacga tcaggacagg gtggcgggga
    14581 tgatgatttg ttgcggttcg ctgcgcccgg ttacctgtgc gatcaattgg caggccgcct
    14641 ggcgtccagc ttcggcgtaa ccaggatcta cggtgacgat ctccggatga aggaatttca
    14701 ttaatggcgt attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc tcttgcaggt
    14761 atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag taaggcggta gtttcaggcg
    14821 taatcacttt tgctacgttc tcatagcctt gcttcatagc aaggcctggc agagcggcaa
    14881 cgggattcag tttatgcgct ttgcagaacg ccaggtaggc ttcgtgacgt cgcttcccgg
    14941 tcgtcacgtc actatgcggc acgccgagat aactgatatt acgatgcccc tggtcataca
    15001 gccgttgcat caggatcttg attgcccctt cgtcgtcata acagaccgaa gcaaaacctt
    15061 ttgcgtcacg cgccagcaga accagcgatg actgccagtg ggctaacatt tcttctgtta
    15121 tgccagtaaa accgaacagc actacgccgt cgatattacg ccgtttcagc acccccaaat
    15181 gttcggcaac taattgcggg gaaaactgac tttccatcat gattgggtcg taaccttgtt
    15241 catagaacgc tggcagcatg gtttgaacgg cgagattttc tgacaacgaa tccaggcggg
    15301 taacaatgat ggcgaccact ttatcgcttt gcccacgcat agcgcgcgca gagcgggaag
    15361 gggaaaatcc atgctgattc atcactgctt caacacgctc gcgggtgcgc tggctcacgc
    15421 cgctttcgtt attcagcacc cgggaaactg tagatttccc cacgccgctt aagcgtgcga
    15481 tgtctttgat ggtcagccga ttttgcatcc tgttgtcctg taacgtgttg tttaattatt
    15541 tgagcctaac tttacccgtg cattcagcaa tgggtaaagt ctggtttatc gttggtttag
    15601 ttgccagcag gtattatatc gccatagatg ctacgaatat tattggattc tccttattat
    15661 ttgcggcgct tttttcactt accggaggtt atatggaacc tgatcccacg cctctccctc
    15721 gacggagatt aaaacttttc cggtaagccc gtcttttcac ggcgttaccg gatgcgtaag
    15781 gccgtgacgt tttaacgtcc ctgctcagct ttattacctt caggtaaggc ttcgccacgc
    15841 ctgaagacat ttctgtactg tttcagacag tgcggaggga ctccttatgt ttaaagaaat
    15901 ttttacccgg ctcattcgcc atttaccttc ccgtctggtt catcgcgatc cattgcctgg
    15961 cgcgcagcag acagtgaata cggcggtccc gccgtcctta agtgcgcatt gcctgaaaat
    16021 ggcggtgatg cccgaagaag aattatggaa aacgttcgac acccatccgg aagggttaaa
    16081 tcaggcggaa gtggaatctg cccgcgaaca acatggtgaa aataaattac ccgcacaaca
    16141 accgtcgccg tggtgggtac atttatgggt ctgctatcgc aaccccttta atattttact
    16201 caccattctc ggcgctattt cttacgccac ggaagattta tttgccgcgg gcgttatcgc
    16261 gctaatggtc gctatttcta cgttgctgaa ctttattcag gaagcacgtt ccactaaagc
    16321 ggcagatgcc ctgaaagcga tggtcagcaa tacagcgacg gttctgcgcg taattaacga
    16381 caaaggcgaa aatggctggc tggagatccc gatcgaccag ctggtgcccg gcgatataat
    16441 aaaactggcg gcgggggata tgatcccggc agatttacgt atcttgcagg cgcgggatct
    16501 gttcgtcgct caggcgtcgt taaccggtga gtctctgccc gtagaaaaag ccgctaccac
    16561 tcgccagccg gagcacagca atccgctgga gtgtgacacc ctgtgtttta tgggcaccac
    16621 cgtggtgagc ggcacggcac aagcaatggt gattgctaca ggtgccaata cctggtttgg
    16681 tcaactggcg gggcgtgtta gtgagcagga aagcgagccg aatgcctttc agcaagggat
    16741 cagccgcgtc agtatgctgc tgattcgctt tatgctggtg atggctccgg tggtgctgtt
    16801 aatcaatggt tacaccaaag gcgactggtg ggaagcggca ctgtttgcgc tttcggtcgc
    16861 ggtaggctta acgccggaaa tgttgccgat gattgttacc tcgacgctgg cgcgcggcgc
    16921 agtaaagctg tcgaaacaga aagtgatcgt caaacatctg gatgctattc agaactttgg
    16981 cgcaatggat attctgtgca ctgataaaac tggcaccctg acacaggaca aaattgtgct
    17041 ggagaatcat accgatatct ccggtaaaac cagcgaacgc gtgctgcata gcgcgtggtt
    17101 gaacagtcat tatcagaccg gacttaaaaa cctgctcgat acagcggtgc tcgaaggtac
    17161 ggatgaagag tcagcgcgct cgctggccag tcgttggcaa aaaattgatg agattccgtt
    17221 tgatttcgag cgtcgccgga tgtcggtggt agtggcagaa aataccgagc accatcagct
    17281 ggtttgcaaa ggtgcattgc aggaaatcct caatgtgtgt tcgcaggtgc gtcacaatgg
    17341 cgagattgtg ccgctcgatg acatcatgct gcgtaagatt aagcgggtta ctgatacgct
    17401 taatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa tacctgccag cgcgtgaagg
    17461 agattaccag cgggcggatg aatccgacct gatcctcgaa ggatatattg ctttccttga
    17521 tccgccaaaa gagacaaccg ctccggcact gaaggcatta aaagcgagtg gaatcaccgt
    17581 aaaaatcctt actggcgaca gtgagttagt tgcagcgaaa gtgtgccatg aagtggggct
    17641 ggacgcggga gaggtggtca ttggtagtga tattgaaacg ctatctgacg acgaactggc
    17701 aaatctcgca cagcgcacca cgctgtttgc ccgcctgacg ccgatgcata aagaacgcat
    17761 tgtgacctta ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg gtattaatga
    17821 tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg gacggcgcgg tagatattgc
    17881 ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg atggtgctgg aagagggggt
    17941 tattgaagga cgtcgcactt tcgccaacat gctgaaatac atcaaaatga cggcgagctc
    18001 caacttcggt aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct tcctgccgat
    18061 gttgccgtta cacttgctta ttcagaacct gctgtatgat gtgtcacagg tggcgatccc
    18121 gtttgataac gtcgacgacg agcaaattca aaagccgcag cgttggaatc cggcggatct
    18181 ggggcgcttt atgatcttct tcggaccgat cagctcgatc ttcgatattc tgacgttttg
    18241 cctgatgtgg tgggtattcc atgccaacac gccggaaacg caaacgctgt tccagtcggg
    18301 atggtttgtg gtgggcttac tgtcgcaaac gctgattgtg catatgatcc gcacccgccg
    18361 tgtgccgttt attcagagct gtgcatcgtg gccgttaatg atcatgaccg tgatcgtgat
    18421 gattgtcggg atcgcattgc cgttttcacc gctggccagt tatctgcagc tgcaggcgct
    18481 gccgttaagc tatttcccgt ggctggttgc gattttggca gggtatatga cgttaaccca
    18541 gttggtgaaa ggattttata gccgtcgtta cggctggcag taagcaagtg atgaggctgg
    18601 cctgaaatac tctttcaggt cagtctttta cgaaaacacc tgaataaaaa aataaattac
    18661 tgcaaaaata aaaaaacaaa taaacgtcca gctcaaatta taaagggcgc tatagctgtt
    18721 atttactgaa cgagagagat agatatcttc ttttttaata tgtctgccag agatgttgcg
    18781 atactggcgg atataataaa cacaatagat taataaaggc ggaattaccc atccacctgt
    18841 aggtttgtca aatataagag tcagtagaat agcgatagca aagaaaacca gaaagacgat
    18901 ttgtgctttg gattctgttt tttttaattc gcttctgatg ccgtcagcgt gctgttcttg
    18961 ccagcgccgc agcgtgctaa cgctaaattg atagtgttca catagctcct tattagacgc
    19021 ccccctcttt tgactgctgt aaaatttcag caatttgttc gttagtgaaa cggggtttag
    19081 ccatcttcaa ccctccgtgg ttgaatcaga taacccaggc gcaatacctg gattatcttt
    19141 cagtagatat tagcgacgaa cagcgatcgc ttcgatctca atcttcacgt ctttcggcag
    19201 acgggcaact tcaacgcaag aacgtgccgg gaaggtggca ttgtgttcgg tgaagaaggc
    19261 ttcgtaggta gcattaacgg ttgcgaagtc gttcagatct ttcacaaaca cggtggtttt
    19321 aacgatgtcg cccactttca ggcctgcggc ttcgacgatc gcttttacgt tatccagcga
    19381 ctgacgtgcc tgtgcagcga cgtctgccgg tacttcgccc gttttcggat ttaccgggat
    19441 ttgaccggag gtgatgatca tattgcccag atcaacgccc tgtacgtaag gaccgatagc
    19501 tgccggtgca ttttccgtcg cgatagtttt gctcatgatt tctcctttat tacagcggta
    19561 aaaagtctgg ctcattatag ggagccagac ttttattacc aaccgcaatt aattggccag
    19621 caccacatta tgggaaaact ctttttcaca gtatttgcat ttgagcgcga tgtcattggc
    19681 gcgttttcgc acggcaaagc tggaagaaac cggttcggca tggctgatac agttgctgtt
    19741 cgggcagacc agcacattgt cgatgcgctc tggcagactg gggcgcgatt tacccaccac
    19801 ttcatagttg tcgatacggt taaccgtggc ttgcggcgca tacaatgcca gttgatctac
    19861 ctgatcttca ctcaaaaagg tattttcgat tttgatcaga tctttgcggc ccatctcgcc
    19921 agaaggcaag ttcagaccga tggtgatgcg ctgatccgtt tcggtcagct tgaacagact
    19981 caacagctta aaaccgatct gggcggggat atggtcaatt accgtgccgc gtttaatagc
    20041 ttcaacctgc aatttattat cgtgtgtcat ctctatttcc ccttacagta ccagatcgcg
    20101 attcagaacc agtgccagta acgcctggcg agcgaaaatc ccgttgcctg cctgctggaa
    20161 gtaccaggcg tgtggcgttt tatcaacatc cgtcgcaatc tcatcaacac gcggcagcgg
    20221 atgcagcact ttcatattgg ctttggcgtt gtggagatcg ctggcgcgaa gaacaaactg
    20281 cgctttcacg ttggcgtact cggacgggtc cagacgctct ttttgcacgc gggtcatgta
    20341 caggatgtct acttccgcca tcacttcttc aatagagctg tgcagactcc atgcgatccc
    20401 tttttcatcg agcatatcca gaatgtattg cggcattgcc agcgcgtccg gcgcgatgaa
    20461 gtaaaaacgg ttgccgtcga acttcgctaa cgcctgagtc agggagtgaa cggtgcggcc
    20521 atatttcagg tcaccaacca ttgcgacgtg gagattgtcc agacgcccct gggtttcctg
    20581 aatagtgaat aagtccagca aggtttgcgt cggatgttgg ttggagccat caccggcatt
    20641 cagtaccggt acattgccgg aaaactcggt ggccaggcgc gccgcacctt cctgcggatg
    20701 acgcatcact atcgcatcga cgtaagtgct gataaccgaa atggtatcgg ccagcgtttc
    20761 gcccttttta cccagtgatg tattggcgct gtcggagaag cccaccacgc tggcacccag
    20821 gcggtgcata gatgtttcga aagagaggcg ggtacgggta gaggcttcga agaaacagct
    20881 ggcaatgact ttgtgcttca acagctctgg ttgcgggttt gctttcagtt tcgccgctgt
    20941 cgccagcacc agattaaggt catcgcgact aaggtcgttt atggaaatga tatgtttctg
    21001 atatagcgga ttagccatct tttatctcct gacgcctggg caaaaaaaag cccctcgatt
    21061 gaggggctgg gaatgggtga tcaacgggaa gaaaaacggc aggccagcgt cttttttcag
    21121 acgcggtaag acaaaatgtc gaacacactg aaccatacat cctcccggca aattgtccgg
    21181 cattatactc atcgtcagaa gcggcgcaag catttgatgc aatattttgt cagcgcaaac
    21241 ggtttatttg aattaaaagt caaggtatat gcatttttat ttgatgtgat tctgcagggg
    21301 aactgtcgtt cggtatcaat aattgcagac attccctgct tttttctttt tcactttcac
    21361 gcaatcagat atgcatttta ttccattcat ccgacttata gggcggagtg tgaaagcgaa
    21421 cggctaacac tattgcttac tgctcaggga tgcgcgctat cactttaatt tcaaaatcaa
    21481 agcctgccag ccatgtaaca cccaccgccg tccagtttgg ataaggtggg gcgctaaata
    21541 tttcattttt caccgtcatg atgtcttcaa attggttttc tggatcggta tggaagctcg
    21601 taacatcaat gatatcgtca aaagtgcatc ccgcagctgc cagggtcgca tgcaaattat
    21661 caaatgccag tctgacttgt tgctgaaaat cgggttctgg tgttccgtcc tctcgacttc
    21721 ctacttgccc ggaaacaaac agcaaatcgc cggaacgaat agccgcagaa taacgatgct
    21781 cagcatatag tgaatgtcgg ccagcaggga aaacagcggt tctttctacc atttggttat
    21841 cctcaagatt tacgacatga acagaagatt tctctttacc gggagccgct tttagcggac
    21901 gacgtgagta aacaaaaccc agacatcatg ggaaatggct gggcttaatt gagcgtagtc
    21961 ggttatgcgc caaacgcgcc atcaatggta tgcatcgcgc cggtaacaaa actggcttct
    22021 ggccctgcta accatgcgac cataccagcg acctcttccg gttgcccatg tcttttgata
    22081 gccataaaac tatgcaacat atcgcgcatt ggcccgttgg cgggattagc gtcggtatca
    22141 attggccctg gctggacgac gttgatggtg atcccacgcg gtccgaaatc acgggccagc
    22201 ccgcgcgcca tgccttgtag ggcagatttg ctggcggcat aagcagccat gcctgcaaca
    22261 ggcatacgat cgccattcac ggagccgatg attaagatgc gcccgccttc gggcatctgc
    22321 cgggcggctt caacagaggc atgataagga gcatgaatat tgattttgaa aaggcgatca
    22381 atatcgtcgg catttaattc cagggcctcg ccaaagacgc caatacctgc atttaccacc
    22441 aggatatcca atgcgccgct cttacgaacg acatcaatga cagcgtctct gtcagcacta
    22501 tctgtgaata ctgctgtcgc tccagtctct tgtgccaggc gtttagcggc atctttcgac
    22561 cccgcatagg tgaatcgtac attggcccca tcggtgacga aacgacgtac gatagcggca
    22621 ccgataccac gactgccacc gaggatgaga actgtcttac ctgtaaaagc gcccataagg
    22681 actccttgat ttattatgta acatgcatta caaaactgtt ttaactttct gtcaacaggt
    22741 tttgtaatgg tcactaaaaa acaatctcgc gttccaggtc gtcccagacg tttcgctcct
    22801 gagcaggcaa tctctgcggc aaaagtgctt tttcaccaaa aaggtttcga tgctgtcagt
    22861 gttgctgaag ttactgatta tcttggtatt aaccccccga gcctctacgc ggcttttggc
    22921 agtaaagctg ggttatttag ccgtgtactc aatgaatacg tcggtacgga agctattccg
    22981 cttgccgata ttcttcgtga tgatcgtcca gtaggcgagt gcctggttga ggtattaaaa
    23041 gaagcggcgc gcagatatag ccaaaacggc ggctgcgctg gctgtatggt tcttgaaggt
    23101 attcatagtc atgatccact agcgcgtgat attgccgttc aatattatca cgccgcagaa
    23161 acgaccattt atgactatat cgccaggcgg catccacaaa gcgcacaatg tgtgactgat
    23221 tttatgagta ccgtgatgtc agggctttct gcgaaggcac gggaggggca ctcaatcgaa
    23281 caactctgtg caacagctgc actggcgggg gaagcgataa aaactcttct caaggagtga
    23341 tgctggcctt gatccgaaag gcgggaacgc gcctgccgat aagttgtgat aagacaataa
    23401 ttcacgcgtt aaggctagcg gaattgatca tcttttcgta taacgataga aatgaaacgt
    23461 tgttttaatt aaggagtgga aaagatgatc atcggaaata ttcataatct tcagccgtgg
    23521 ctaccccagg agttacgcca ggcgattgag catatcaaag cacacgttac ggcagaaacg
    23581 ccaaagggca agcacgatat cgaaggcaat cgtctgtttt atcttatctc ggaagatatg
    23641 accgagccgt acgaagcccg ccgtgcggag taccatgccc gctatctcga cattcagatt
    23701 gtgttaaaag gtcaggaagg catgaccttc agcacgcaac ctgcaggcac gccggatacc
    23761 gactggttag ctgataaaga catcgcattt ttgccggaag gcgttgatga gaaaacagtt
    23821 atccttaatg aaggtgattt tgttgtgttt tatccggggg aagtgcataa accgctgtgc
    23881 gcagtgggtg caccagccca ggttcgcaaa gcagtagtga agatgctgat ggcgtgatga
    23941 cttttcgccg taaataactc cagatttacg gcgagtttgt gaaaagagcg ttttttgata
    24001 tttttttgtg agtaaaattt gtaatgctta gacgttctta ttaactcaag gagtccgtca
    24061 tgagcaaaat atcaggttgg aatttttctc aaaacattac atcagccgac aattgtaaac
    24121 aaaaaaatga agacttagat acctggtatg tgggaatgaa tgattttgcc cgaattgccg
    24181 gagggcagaa tagcagaagc aatattcttt ctcccagagc atttttggag tttttggcta
    24241 agatatttac cctgggttat gtggatttta gcaaacgctc caacgaagcg ggtagaaata
    24301 tgatggctca tattaagtcc tcatcttata tcaaaaataa tgatggtagt gagataatga
    24361 agtttgttat gaataatcct gaaggggagc gagcggattt atctaaggtg gaaatagaaa
    24421 ttacacttgc ctcagctttt aataatggga ttcgtgaagg acatacagtc attatattta
    24481 cacaacctga tggttcgact aatcgttatg aagggaagtc ctttgaaaga aaagatgaga
    24541 gttcattaca cctgattact aacaagattc tggcgtgtta ccaacgcgaa gctaacaagg
    24601 aaatagctcg tctattaaat attccgcagg agttaaataa ttctcaggac ttgaataact
    24661 cgcaggtgag ttgtaaagat tcagttgatt ctacgattac ggatttatta gaaaaaccat
    24721 tgaataatgc attattagca ataaggaaag aacatctgct attaatgcct tatgtatgta
    24781 atgaatcgat ttcatacttg cttggtgaaa aagggatact gaaagaaata gatgacctta
    24841 acgcagtaaa taattatctt cttaataata agaaagctac tgacaatgaa attaatgaca
    24901 tcaaagttaa cctgtctcat attcttattg attccttaga tgatgcaaaa gttaacctta
    24961 caccggtcat cgattcgatt ctggagattt ttaaaattcc catatattaa tgatgtaaga
    25021 atactggatt gatgttttaa taaaagaatg caatattttg gtgattctga aaagataaag
    25081 tatgcatgct ccgtgataaa tcatattgat tttagccgcg atcaatctaa agattttagc
    25141 tgcgatcaat ctaaaattaa aatagctgag accttatttt tcaatctcga taaagaacac
    25201 tataaaaata gccgtaaatt acaggaattg atttgggata agttggttgc atatgtcaat
    25261 gattttaact taagcaatca agaaaaatca cgcttaatac taaggttatt tgatgatgtt
    25321 aagttgctat ttaatgaagt gcctgtcagc attttagtaa atgatatttt ttttgaagga
    25381 ttttttcatg aagcaacctg attttgctaa gtggtatttc tatcaattac ttaaagatta
    25441 tgagggcgaa cagctgtatc ttaatgagtt aggatatgtt tatggtaatg aagaaaaaac
    25501 aaatgaaatt gtaaaaaaca atccgggtta tgtcgttaag atttttgaag aaaagatggt
    25561 taatgaatta aaaattagaa caagaatgat gaaaatcctc cggaagattt acgtatagca
    25621 atagaaaaat atggatggaa taattgaact attactgcat aaaataatgt gatgccggga
    25681 tgtttttgta tttcccggca tctttatagc gatatcaatt atttactgag cgtcgcgacc
    25741 atcaccgctt tgatggtatg catgcggttt tccgcctgat caaaaacaat gctggcggca
    25801 gattcgaaga cctcatcagt cacttccatt ccgccatgta ggccaaactc ttccgccatt
    25861 ttcttaccaa gcgtcgtctg gtcgtcatga aacgcgggca ggcagtggag gaatttgacc
    25921 tccgggttac cggtcaactg catcatcttg ctgttcacct gataatcacg cagtaatgca
    25981 atccgttctg cccatttctc ttttgcttcc cccatcgaca cccatacatc ggtatagata
    26041 aagtcagcac cttcaactcc tttggctaca tcttccgtca gcgtaatatt cccgccattt
    26101 tgctgtgcca gggcgcggca ttccgtaacc agcgcagctt ccggccagca tgcctgtggc
    26161 gcgaccagac gcaaatccag accggtaagc gccgcagctt cgagcatcga attgcccatg
    26221 ttgttacgtg cgtcacctgc atagaccagc gtcatttcgt tgaatgcttt accgggtaaa
    26281 tgctcctgca tggtgaggag atccgccagc aactgcgtgg ggtgaaactc attggtcagg
    26341 ccgttccata ccggcacgcc agcgtattcc gccagtgttt cgacaatctc ctgaccatag
    26401 ccgcgatact gaataccgtc atacatgcga cccagcacgc gggcagtgtc tttaatcgac
    26461 tctttatgac caatctgact gccgcttggg ccgagataag taacgcgagc accctggtca
    26521 tatgcggcaa cttcgaaaga gcatcgggta cgggtcgagt ctttttcgaa gatgagcgcg
    26581 atgtttttac cagtgagttt ggcttcttct ttgccgcttt tcttatcggc tttcagcttc
    26641 gcggctaact gcagcaggct gttgagttca gctggcgtga aatcgagtaa tttcaggaaa
    26701 tgcttatgat aaaacccgga catagatccc tcctgtggct aacgccttaa tgaattaaaa
    26761 ttcaatttat atggatgatt attcatttgc aagtctaaag cataaatctt tgtcacaaag
    26821 gtggaggcaa tgtcagtggt gtgtgacaat aagagtatcg gcaggacatt aagaggaatg
    26881 agccatggca aacccggaac aactggaaga acagcgtgaa gaaacacgtt tgattattga
    26941 agaattactg gaagatggca gcgatccgga cgcgctgtac accatcgaac accatctttc
    27001 cgcagacgat ctggaaaccc tggaaaaagc agcagttgaa gcgtttaaac tcggttacga
    27061 agtgaccgat ccagaagagc tggaagttga agatggtgat atcgtgattt gctgcgacat
    27121 cctcagcgag tgcgcgttga atgccgatct gatcgatgcc caggttgaac aactgatgac
    27181 gctggcggag aaatttgacg ttgagtacga cggttggggc acttactttg aagatcccaa
    27241 tggcgaagac ggcgacgatg aagattttgt cgatgaagac gatgacgggg ttcgccacta
    27301 attaattgat taaaatgccc gatagcacac actagcgggc atttttttag agttcgcgta
    27361 gcatccgcac ttcacaatct acatggccgt gcagccaagt gcatagtcga tatgttgaaa
    27421 acctaaatgt tcatacaaac caatcgcttc tttcaaaaaa gcggtcgttt ccagatagca
    27481 gcgtttgaag cccatctctc gtgcctgctc catcgccatt aaggccagtt ttttcgccag
    27541 ccctttaccg cgaatagccg gaagaaaata catcttctgc agttcgcaaa tgtccgactc
    27601 actcccggtt aatggcgcaa tcccaccgcc gccgaccact tcaccatcgt actcaaccac
    27661 ccaatatgca tgacccggct gactatacac ttgatacaac tcgtccaggt tcggatcggc
    27721 gacggtgtag cctttatcag cggtaagacc gtattcggcg gatacctgac gaatgacgcg
    27781 ggcgatggcg ggattatcct gctgcgtcag gcgacgcatt acaggtgatt gcggcgcaat
    27841 gttattcata gtttaaccca ataaaaataa agtaattata catttgttta ataccactct
    27901 cttcttacgt gcgcaagcga cttataaagt aataaagttt ttgccagcct attactgata
    27961 agagtattgt aacgtggcag aacattttac tgtctgtata tataaattat atcaatggat
    28021 tttttaattc agggaatctt tatggctcaa gttattaatg aaatggatgt tccgtcccat
    28081 tcgtttgttt ttcatggtac aggtgagaga tattttctta tttgtgtggt gaatgtgttg
    28141 ttaacgatta taacgctagg tatctatttg ccatgggcat taatgaaatg taagcgttat
    28201 ctctatgcta atatggaagt taacggacaa cgattttctt atggaattac tggtgggaat
    28261 gtttttgtta gttgtcttgt ttttgttttt tgctatttcg caatcttaat gacagtgtca
    28321 gcagatatgc cacttgttgg ctgtgttttg actttgttac tgttggtttt gcttatattt
    28381 atggcagcaa aaggactgcg ttatcaggcc ttgatgacca gtctcaacgg cgtaagattt
    28441 agttttaatt gctctatgaa agggttctgg tgggtaacct ttttcttgcc gattttaatg
    28501 gccattggga tggggactgt tttctttatc tcgacaaaga tgctacatgc caatagttca
    28561 agtagtgtta ttatatctgt ggttctgatg gcaatagttg gtattgtttc cattggtatt
    28621 tttaatggta ctttatatag cctggtaatg agttttctct ggagcaatac cagtttcggt
    28681 atacatcgtt tcaaggtgaa attagatact acgtattgta taaaatatgc cattctcgca
    28741 tttttagctt tattaccttt tctcgctgtt gctggttata ttatcttcga tcaaatatta
    28801 aatgcgtatg atagttctgt gtatgcaaat gatgatattg agaatttaca gcaatttatg
    28861 gaaatgcaac gtaaaatgat aatcgcgcag ttaatctatt attttgggat tgctgttagc
    28921 accagttatt taacggtgtc gttgcgaaat cattttatga gcaacctgtc actgaatgat
    28981 gggcgtattc gttttcgctc aactttaacg taccacggta tgctttatcg catgtgtgcg
    29041 ttggtggtga tatccgggat tacgggcggt ctggcttatc cactgctgaa aatatggatg
    29101 attgactggc aggcaaaaaa tacgtatttg ctgggcgatt tggatgacct tcctttaatc
    29161 aataaagaag aacaaccaga taaaggcttc ttagccagga tttcacgggg aattatgcct
    29221 tctttaccat ttctgtaaga gataaaaaag gccggagcat gctccggcct tcgttttcat
    29281 tactgtgttt tgattacagc gcggcgataa cagcctgctg ttcaatcagc ttcgctttcg
    29341 cttctgcata gccttccagc ttttcacgct ctttcgcgat gaccgcttcc ggtgcgcggg
    29401 cgacaaagcc ttcattcgcc agcttgttct cgatacggct gatttcacct tcaatcttcg
    29461 ccacttcttt cgccagacgc gccagctcat cttctttgtt gatgaggcca gccatcggga
    29521 tcagcagctc tgcaccgtcg atgatcttag taacggaaac cggacctttg tcatcggcag
    29581 gcagcacggt gatactttcc agacgcgcca gcgtttgcag gaagccacgg ttttcattta
    29641 cgcgacgttc tgcatccgcg ctgcaaccac gcagtagcag ctccagcggt ttgcccggcg
    29701 cgatgttcat ttctgcacgg atgttacgta ccgcaacgat cgcctgcttc agccactcgg
    29761 tgtcggccag tgcggcttca tcaacctgag atgcatcgta ctgcgggaac ggctgcagca
    29821 tgatggtgtc ggcagtgata ccgcaaagta ctttcacacg ctgccagatg gtttcggtga
    29881 tgaacggaat gatcggatgc gcgaggcgca gcagaccttc cagtacagtc accagcgtat
    29941 ggcgagtacc gcgcagttct gcttcggtgc caccgttcat taccggcttg gtcagctcga
    30001 gataccagtc acagaactgg ttccaggtga actcatacag aatgcctgcg gcgatatcga
    30061 agcggaagct gtccagcgct tcgcggtacg ctttgatggt ctggttgaac tccgccagaa
    30121 tccagcggtc cgccagcgac agcgtcattt cgccgccgtt gaagccgcaa tcctgacctt
    30181 ctgtgttcat cagcacaaag cggctggcgt tccacagctt gttacagaag ttacggtaac
    30241 cttccaggcg cttcatatcc cagttgatgt cacgaccggt agacgccagt gccgccaggg
    30301 tgaagcgcag cgcgtcagta ccgtgcggtt caataccgtt cgggaactgc ttctcggtgc
    30361 gcttacggat tttgtccgcc agctgtggct gcatcatatt gccggtgcgt ttttccagca
    30421 gttctggcag cgaaataccg tcaaccatat ccagtgggtc aataacgtta cccttggatt
    30481 tggacatctt ctggccttcg tcgtcacgaa tcaggccggt catgtaaacg gtgtggaacg
    30541 gcacctgcgg tttgccattt tcatctttga tgaagtgcat ggtcatcatg atcatgcggg
    30601 caatccagaa gaagatgatg tcgaagccgg ataccatcac gctggttggg tggaactgac
    30661 gcagggcgtc ggtgttttcc ggccagccta gggtagagaa ggtccacagt gcagaagaga
    30721 accaggtgtc gagaacgtct tcgtcctgac gcagggcaac gtcggcaccg aggttatttt
    30781 ctttacgcac ttcgtcttcg ttgcggccaa cataaacgtt acccgcttcg tcataccatg
    30841 ccgggatacg gtgaccccac cacaactgac gagagataca ccagtcctga atatcgcgca
    30901 tccaggagaa gtacatgttt tcgtactgct tcggtacgaa ctgaatgtcg ccgttctcaa
    30961 ccgcttcaac tgccggtttc gccaggacat cggcacgcac gtaccactgg tcggtcagca
    31021 ttggttcgat gaccacgccg ccacggtcgc cgtaaggaac ggtcaggtca tgtggtttaa
    31081 tttcttccag caggccgagt gcgtcaaccg cagcaacgac cgctttacgt gcagcaaaac
    31141 gctccagttt ctggaattct gccgggatct cgctggaata aacgtcagat tcgttacctt
    31201 tggtatcgaa cacctgggca ctttcacgga tatcgccgtc aaaggtcagg atgttgatca
    31261 tcggcagggc gtgacgttta ccgacttcat agtcgttaaa gtcgtgcgcc ggggtgatct
    31321 tcacgcagcc ggtgcctttt tccatgtcgg cgtgttcgtc gccaacgatc ggaatacgac
    31381 ggttaaccag cggcagaatg acatatttgc caatcagatc tttgtaacgc ggatcttccg
    31441 ggttaacggc tacgccagta tcgcccagca gggtttccgg acgggtagtc gcaaccacca
    31501 gataatcttt accgtctgcg gttttcgcgc cgtcagccag cggatagcgg atgtgccaca
    31561 tcgaaccttt cgattcgcgg ttttccactt ccaggtcaga gatagcggtg cgcagtttcg
    31621 ggtcccagtt taccaggcgt ttgccacggt aaatcaggtc ttctttatac agacgaacga
    31681 aaacttcttt caccgcattg gacaggcctt cgtccatggt gaagcgttca cgctcccagt
    31741 ccacggagtt gccgagacgg cgcatctgac gggtaatggt gccgccagat tccgctttcc
    31801 attcccagat tttgtcgatg aaagcttcgc ggccgtagtc gtgacgggtt ttaccttctt
    31861 ctgcggcaat cttgcgctca acgaccatct gggtagcgat cccggcgtgg tcagtaccga
    31921 cctgccacag ggtgtttttg ccctgcatgc gctgatagcg gatcatggta tccatgatgg
    31981 tttgctggaa ggcgtgaccc atatgcaaac tgccggtgac gttcggcggc gggatcatga
    32041 tgcagaaact ttcctggctt tcatcgccat taggcttaaa gtagccctgc ttttcccagt
    32101 gctcgtaaag cggctgttcg atatcttgtg ggttatatgt cttttccatt atttccaggt
    32161 tgccgtattc aggttgaaac cagccacgcg gtaggcttta tagcgttcgc gcgccagttg
    32221 tttcagagaa tcttcataag gaacgaagtc taccacttct gtgaaagcgg tggcaaaatc
    32281 tgcaaagctt gttcgcagac taatcaatat atcgcgcggg ctgctgctgc gtttttgcgg
    32341 ccaggcgatc tccaccggcg caccgccgcg cggtccttct cccgctaaat tatgcggaac
    32401 aaagctttct gccggacgcg cccacagggc ttcatccagc cggtaagcct gcttttcatc
    32461 ttcacaggcg atgagtacgc gcttaccact gcgccaacgt tctgcggcaa tttcacacac
    32521 cagttgctca acggcgctta agccatcgac ggtggtgtca ttgtccagaa ggtagaacgt
    32581 cgcgtttttc ataaatgggg cttcttgtgg tggatttaaa tattgctgca ttcctcaccc
    32641 taaaagatga gcaatgaatt tctattgcgc tcgatcggtt ccctctccct tagggagagg
    32701 gttagggtga gggtaaaacg caaacttact cttcgccgtt aaacccggcg cggtttaaca
    32761 ggaactgtgc cagcaacgct accggacgac cggttgcgcc tttcgcttta ccagaacgcc
    32821 aggcggtacc ggcgatatcc aggtgcgccc agttgtactt acgggtaaag cgtgacagga
    32881 agcaacctgc ggtaatcgcc ccaccaggac gaccgccaat gttcgccata tcggcaaaat
    32941 tggactccag ttgttcctga tactcgtcac ccagcggtaa gcgccatgcg cggtcaccgg
    33001 attgttcaga cgcggcaatc agttcatggg ccagcggatt gtggttcgcc atcagaccag
    33061 taatgtgatg acccagcgcg atcacgcagg caccggtcag cgtcgccacg tcaatcaccg
    33121 cttccggctc aaaacgctca acgtaagtta acacgtcgca cagtaccagg cggccttcag
    33181 cgtcggtgtt gagcacttca acggtttgac cagacatggt ggttaacaca tcgcccggac
    33241 gataggcgcg accgcctggc atgttttcac aacctgccag cacgccgata acattaatcg
    33301 gcagttgtag ctccgcgacc atacgcatca cgccgtaaac cgccgccgcg ccgcacatat
    33361 cgtacttcat ctcatccatg ccttctgaag gcttgatcga gataccgccg gagtcgaagg
    33421 ttaaaccttt acccaccagc acgattgggc gtgcatcttc cgacgcgttg cctttgtact
    33481 caatcaccga catcagcgat tcgttttgcg aaccctgacc gaccgccaga taagagtgca
    33541 tccccagctc tttcatctgc tgttcgccga taacgcgggt aatgacattc ttgctgtagc
    33601 tgtcagccag ctggcgcgct tgtgaagcga ggtaagcggc gttacagata ttcggcggca
    33661 tattgccgag atcttttgct gctttaatcc cggcggcaat cgccagaccg tgctggatcg
    33721 cgcgctcacc gctggtcagt tcacggcggg tcggcacgtt gaacaccatc ttacgcagcg
    33781 gacgacgcgg ttcgctcttg ttcgttttca gctgatcgaa actgtagagc gtctcttttg
    33841 ccgtctcgac agcctgacgc actttccagt agttgttacg gcctttaacg tgcagttcag
    33901 tcagaaagca gaccgcttcc attgagccag tatcattcag cgtattaatg gttttctgaa
    33961 taacctgctt gtactgacgc tcatccagct cacgttcttt gccgcaacca ataaggagaa
    34021 ttcgctcgga aagtacattc ggaacatggt gcagcaacaa tgtctgcccc ggttttcctt
    34081 ccagttcgcc ccgacgtagc agggcgctga tgtacccatc gctgatttta tcgagctgtt
    34141 ctgcaatcgg agaaaggcga cgtggttcga agacgcccac gacgatgcag gcactccgct
    34201 gtttctccgg gctaccgctt tttacactaa actccatgca ctacgctcct gaatcttaaa
    34261 gacaacggcg gtggctacag atagaattgc aagctttcgt aactcatgtc cgctgttgcg
    34321 atgacttcgt gttaatctta acgttattac ggcattggca cgtcagaaca aaatctgaga
    34381 ggtgaatccg ttgagtataa ttatcttagc gacgatttcg acgactcaag agaataaatg
    34441 acgtttaagc catgaaacaa gctaaaatcc tgcaaaagac gagtttttac gggcgtattt
    34501 aaagtgataa tcataagata tctggtgcgg gagacgctca aaagccagct ggcgatactc
    34561 ttcatcttgc ttttgatctt cttctgtcaa aagttagtga ggatcctcgg cgcagcggtt
    34621 gacggcgata ttccggcgaa tctggtgctc tcccttctcg ggttgggcgt gccggaaatg
    34681 gcgcagctta tcctgccatt aagcctgttc ctcgggctgc tgatgacgct gggcaaactg
    34741 tataccgaaa gtgaaattac ggtaatgcat gcctgcggcc tgagcaaagc ggttctggtg
    34801 aaagcggcaa tgatccttgc ggtattcacg gcaatcgtcg cggcggttaa cgtgatgtgg
    34861 gcgggaccgt ggtcatcgcg tcatcaggat gaagtgttag cagaagcgaa agcgaaccct
    34921 ggcatggcgg cgctggcgca agggcaattc cagcaagcga ctaatggcag ttcggtgctg
    34981 ttcatcgaaa gcgttgacgg cagcgatttc aaagatgtgt tcctcgcgca aattcgacca
    35041 aaaggtaatg cacgtccttc tgtggtggtg gccgattccg gacatttaac ccagctgcgc
    35101 gacggctccc aggtcgtcac tctcaaccag ggaacgcgct tcgaaggcac tgcactgtta
    35161 cgtgatttcc gcattacgga tttccaggat tatcaggcga tcattggtca ccaggcggtg
    35221 gcgctcgacc cgaacgatac cgaccagatg gacatgcgta cattgtggaa cactgacacc
    35281 gatcgtgccc gcgcagaact gaactggcgt atcacgttgg tattcaccgt gtttatgatg
    35341 gcacttatgg tcgtaccgct gagcgtggtt aacccacgtc agggacgcgt actgtcgatg
    35401 ctgccagcca tgctgctgta tctacttttc ttcctgatcc agacctccct gaaatcgaac
    35461 ggtggtaaag gtaagctgga cccgacgctg tggatgtgga ccgttaacct gatttatctg
    35521 gctttggcga ttgttctcaa cctttgggac accgtgccgg tccgccgcct gcgcgccagt
    35581 ttttcgcgta aaggagcggt gtgatgcaac cttttggcgt acttgaccgc tatatcggta
    35641 aaactatttt caccaccatc atgatgacgc tgttcatgct ggtgtcgctg tcgggcatta
    35701 tcaagtttgt cgatcagctg aaaaaagccg ggcaggggag ttacgacgcg ttaggcgcag
    35761 gaatgtatac cttgctgagc gtgccgaaag atgtgcaaat cttcttcccg atggcggctc
    35821 tgcttggggc gttgcttggt cttgggatgc tggcgcagcg cagcgaactg gtggtgatgc
    35881 aggcttctgg ttttacccgt atgcaggtgg cgctgtcggt gatgaaaacc gccattccgc
    35941 tggtcttgct gacgatggcg attggcgaat gggtcgcgcc gcagggcgaa cagatggcgc
    36001 gtaactaccg tgcgcaggcg atgtacggcg gctcgttgct ctctactcag caaggcttat
    36061 gggcgaaaga tggcaacaac ttcgtctaca ttgagcgggt taaaggtgac gaagagttag
    36121 gtggcatcag tatttatgcc tttaacgaga atcgtcgtct gcaatccgta cgctatgccg
    36181 ctaccgcgaa gtttgacccg gaacataaag tctggcgtct gtcgcaggtt gatgaatctg
    36241 atctgaccaa tccgaaacag attaccggtt cgcagacggt gagcggcacc tggaaaacca
    36301 acctcacgcc agacaaactg ggcgtggtgg cgctggaccc ggatgcactc tctatcagcg
    36361 gtttgcacaa ctatgtgaag tatctgaagt cgagcggtca ggatgccgga cgttatcagc
    36421 tcaacatgtg gagcaaaatc ttccagccgc tatctgtggc ggtgatgatg ctgatggcgc
    36481 tgtcgttcat ctttggccca ctgcgtagcg taccgatggg cgtgcgtgtg gtcaccggta
    36541 tcagtttcgg ttttgtcttc tacgtactgg accagatctt cggtccgctg acgttggttt
    36601 atggtatccc gccgatcatc ggcgcactgt tgccaagcgc cagcttcttc ttaatcagcc
    36661 tgtggctgtt aatgagaaaa tcgtaaccga atgaacgaat aaagggagcg aaagctccct
    36721 ttattattgt tagcaaagtg tgcttcgttc attcctgaaa aattatgaaa atccaatata
    36781 ttgcagtggt gtaggcatga taagacgcgc cagcgtcgca tcaggcaaag acaaatcgca
    36841 cctggcttac ctttttctcc cccccagcaa actccccaac attccacgca caatctgatt
    36901 cgtcacctgg cgagcggcgc ttttggccat tgtttgcacc acaccatctt tcttcccgcc
    36961 gcgtggtccg gtagtgccaa acaaaatatc cttcaatcca ccgagaatac cgtcatccac
    37021 cgccacctct ttccctttcg cgggaggatt attttgctgc tcggtactgg cctgaaagcc
    37081 tttttgcaac atctcatagg cggattcccg gtccacgtca tcctcgtact tgccatacac
    37141 cggggagtga ttaatcaggc cgttacgctc atcttccgtc accggcccca ttcgcgaaca
    37201 gggggcaatc accatcgccc gttccaccac tgaaggactt cctttcgcat cgagaaacga
    37261 gattaacgcc tcgccggtgc ccagttcctg aatcgccttt tcggtatcaa acaccgaatt
    37321 ggcccgcatg gtttgcgccg cagcttttac tgctttctga tctttgggcg taaaagcccg
    37381 caaagcgtgt tgaacgcgat tacctagctg cccgagcaca ttatccggaa tatctgacgg
    37441 gttttgcgaa acgaaccaga cgcccacgcc ttttgagcga ataagcctta tcacctgctc
    37501 aatcttatcc agcagtacct gtggcgcatc gttaaacagc agatgtgctt cgtcgaagaa
    37561 aaacaccagt tttggtttct ccagatcgcc cgcttcaggc aattgttcat acaattctga
    37621 aagcatccac agcaggctgg cggcgtacag tttcggcatc tgataaagct tctcggcgct
    37681 gaggatattg ataacgcctt taccgttggc atcggtacgc atccagtctt tgatatccag
    37741 catcggctca ccaaaaaagt gcgccgcgcc ttgctgttcc agcgataaca gcccgcgctg
    37801 gatggcacca accgatgcgc tactgatatt accgtactga ttctggaaag atttggcgtt
    37861 atcgccgatg tactgggtaa tcgcccgcag atctttaaag tcgagcagca acaatccctg
    37921 atcgtcagca atacggaaga tgatattcag cacgccagat tgcacatcgt tgagattcaa
    37981 cagccgcgcc agcaacagcg gccccagatc cgaaaccgtc gcccgcaccg gatggccttt
    38041 ctcgccaaag atatcccaca ccaccaccgg attagcatgc ggttgccagt cattgacgcc
    38101 gatattttta agccttgcga gtagtttttc cgatgccgtt cctgcctgcg cgatgccggt
    38161 cagatcgcct ttcacatcgg ccataaacac cggaacaccg atttccgata gagactccgc
    38221 cagtttttgc agcgtgacag ttttacccgt ccccgttgcg ccagtaatca gcccgtgacg
    38281 gttagccatt cccggcagta aaaacagttc tgtgtccggc gtgcgggcaa ttaacagggg
    38341 ttcgctcatg agatatcctc cagtttcctg cctggagtat aggcaacggc tgtgcactct
    38401 tcaccgctaa attcagcaga ttgcgagacg ccttccaaag agatttaagt gccgggcgac
    38461 aggcacccgg caaagggatc agaagtcggc tttcagaacc acgcgataac gcgccttacc
    38521 gtcgcgcaca tgctggatgg cgtcgttaat tttcgacatc gggaacagtt cggtggtcgg
    38581 cgcaaccttg ctgcgggcgg caaaacgcat cagcttacgc agctcataag gtgtgccggt
    38641 tgctgaacca gagacgctgc ggtcgcccgc aattaacgta aaggccggaa cagacagcgg
    38701 cgtgagaacc gcaccgaccg tatggaaatt accgccatag gtcagcgcct caaaataagg
    38761 ctgccagtcg aggctgacgt tgacggtgtt gataatgaga tcaaactgcc ccgccagtgc
    38821 tttcagtgcc tgcggatcgc ggctattcac cactttatcg gcacccatcg ccagcacttc
    38881 ctgctctttc gccggattag aactaaaggc tgtcacctcg catcccattg cgtgcagaag
    38941 ttttatagcg atatgcccca gcccgccaat accaattacc ccaacgcggc tggtggcagt
    39001 gatatggtgc atcaacagtg gtttaaagac cgtgataccg ccgcacaaca gcggcccggc
    39061 agactcgata tcaatatttt ctggcagtgg aatcacccat tgccagtccg cacgcaactt
    39121 ctcggcaaaa ccgccgcgat tcataattgt tggcaccgca ccttgctcgc agttgatctg
    39181 attaccgcta atacaggcat cgcaatgccc gcagctgcgt gccgtccagc caatcccgac
    39241 acgctgaccg acctgcaaac ctttatcctg tgcggcgctc ccgagcgcca ccacgcgacc
    39301 aatcacctca tgcccggcaa ccagcggata ctgtgaaaat ccccattcgt tgtcgatcat
    39361 cgacagatcg gaatggcaga tcccgcagta atccacctgc acttcaacat cttgtggctt
    39421 cagttcaccg ggatcgtact cataaacttc cagttcgccg cccgcttctt ttgcggcata
    39481 gcattttatc atcgacattt tttgatcctt ctctggtgtt gtctgggaag tgtagagcat
    39541 ggcagggcgg ggtgcctgga gtgtgacaaa ggttacacat cgctgtatgc aatgctgaaa
    39601 atttcagcac ttagcgaagt gggagcaagc tggcgcttgc atggtggcgt gcgccaggta
    39661 taatccacaa cgttttccgc atacctcttc agtgccgaag tggcgaaatc ggtagacgca
    39721 gttgattcaa aatcaaccgt agaaatacgt gccggttcga gtccggcctt cggcaccatt
    39781 cttacttcca agaccatccg agaaagtcca aatatccctt taaaatcaat gcttgcagcg
    39841 attttttcgt cctgcgtctt ccgaggttgt ccgttgaaat ccggatatca ttaggggcat
    39901 aattgggggc atcttaactt cgattagaaa tgtgccccca aatgaagctc aacgccagac
    39961 aggtcgagac cgcaaagcca aaagacaaaa cctacaaaat ggccgatggt ggtggtttgt
    40021 atcttgaggt ttcggccaaa ggttccaaat actggcgcat gaaatacaga cgtccctctg
    40081 acaaaaaaga ggatcgtctt gcgtttggtg tttggcctac tgtgacgctt gctcaggcaa
    40141 gagcaaaacg cgatgaagct aaaaagcttt tagtacaggg catagaccca aaagtcgtac
    40201 aaaaagaagc tcgggccgag aattcggggg catatacttt tgaagctatc gcccgagaat
    40261 ggcatgccag taacaagcgc tggagtgaag accatcgatc gcgcgttctt cgctatcttg
    40321 agctttatat attccctcat atcggttcgt ccgacattcg gcagcttaaa accagccacc
    40381 tgttagcccc gattaaaaaa gttgatgcca gtggcaaaca cgatgtcgct cagcgcctgc
    40441 aacagcgcgt cacagccatt atgcgttatg ccgtacagaa cgattacatc gactcaaatc
    40501 cagccagcga tatggctggt gcgctatcga caaccaaagc gcgacattac cctgctttac
    40561 cttctagccg attccctgag tttcttgcac gtcttgctgc atatcgtggc cgtgtaatga
    40621 cacggattgc ggtcgagctt tccttgctaa cttttgtgcg ttccagtgaa ttacgtttcg
    40681 cacgttggga tgaattcgac ttcgataaat ctctttggcg tatacctgca aagcgagaag
    40741 aaattaaagg tgtgcgttat tcgtaccgag gcatgaagat gaaagaggaa catatcgttc
    40801 cgcttagtcg acaggcgatg attttattaa accagttaaa gcagattagt ggtgataaag
    40861 agctgctttt tcctggggat catgacgcaa ctaaggttat gagtgaaaac acggtaaaca
    40921 gcgcattgcg tgcgatgggc tatgatacta aaaccgaggt gtgtgggcat gggtttagga
    40981 ctatggcgcg tggtgcgttg ggggagtcgg ggttatggag tgatgacgcg atagagaggc
    41041 aactgagcca ctcagagcgt aataatgtac gtgcggcata tatccatacc tccgagcatt
    41101 tggatgagcg gcgtttaatg atgcagtggt gggctgatta tttggatatg aaccgtaata
    41161 aatatattac gccttatgat tattcaaaat acaaagaaat acttaaataa aaatagttat
    41221 tggttgattt ttaaaatgag tgttaagtaa cataatctgg agtttttaat tgttagttgc
    41281 tataataaaa atcaacgtga agtgagtgtg attaacatgg ctgagttatt gcgagagcag
    41341 gaagttaaaa tagccctaat tgaatggtta tataaaaaag gcatgcttag tgatgcaacg
    41401 atcattaatg aaatggttgt tgctaactgg agcagaagag ctgatttggc tattgccaat
    41461 ggaagcctac aagcttttga aataaaaagc gattttgact ctcttaagcg cttggatggt
    41521 cagttaaaaa tttttaaatc tagcttcgaa aaagttacaa ttgtgtgcgc gccaaaattt
    41581 accagtgaag tatctaaacg ggtttcatca gatgttggcg ttgtagaatt tcaatctaat
    41641 aatagtagcg tacgctttaa aataattcaa aaaggaaggg tttgctctcg cttaaataaa
    41701 tttgtttatt tgggtttttt gcttaagtct gagcttaaag atctgcttaa tcagtatgga
    41761 attacatttt caaatgaact acatagggat tgtcttgagg ttttggcatc taagttatct
    41821 ttaaacaaga ttagagaatt tgtcattaca tctattaaaa ataggtatca tgcgacatct
    41881 aatgattttt tatctagact ccatggtaat caagaaatat ctataaacga tcttgcctta
    41941 ttgagcaaag ctaaaagaag gaataattct ttggttaagt ttgagtttaa ggcttgttcg
    42001 gagaaatctt taaatgaata ccataaggtt gatgttgact ctatagttag gaaatatggc
    42061 gatgatgttt cctatattcc ggaaaaagta cttaaaaggg ttgtgggcta gggaaggcta
    42121 accttcccta gttttttatt agataagatc ttcctcatca tgatcaaagc catactgtag
    42181 agcttcagat agctcaattt gcttatttaa gtgtagattc actcgaacag caatccattt
    42241 agctggactt cccatacctg ctatatcccc tatagcagca tttcttatca ttgcagctcc
    42301 ccaagagtcc tccctttgat aatgtggata ctctgccaaa attgatttcg cagcttcaat
    42361 gaacccttct ttattcatcc cagggcggcg ctcgaaatac catgaatcat ttaacgctat
    42421 atcaattcta ggaacatatc gcccaattat gttgtcatac actactgaat gaattgaacc
    42481 atgatcgcca taaatagcga catcacttcc accaatattt tgatgcaatt ctctctcaat
    42541 aacatcaata tatccggact gtccattttc acggcaaaaa tttgtaactg aactagggaa
    42601 gcttgttgct agcacactaa ttatggcttc aggaatctca gttctaatct gattaatcga
    42661 atttattgct gcagctgtta tagcagatac attccctcta atgtagccca aatctatgaa
    42721 aactatggca ttttctggag agttcatcga tactagtgac gtcagggttt tattaatgtc
    42781 tgtatttaga tttctaattc taaatgcaat tgaaccttta agttcctcta aaaccctggc
    42841 ttggatagat atatctctaa gttttgctga atccggcatc tggactactg gaataatatt
    42901 atcatttctg gaacaaaact ctatccagtt tttaaaacca ttctcaggag agagtagttc
    42961 gaagcttgac gcgcaatgat gagaattatc ctttgtgaca tccaaaataa atgggagatt
    43021 actcatcact tctaaacttt tatctaacga tacctgtatt tcttccgagc gaggccattt
    43081 ccccaaagaa attaaaggta atattttttt cttattctca tatgataaat tattcagtcc
    43141 ggtcatttcc gctgatcgtg ttcttagggc tggatagtat gagtaatttt caaaatatac
    43201 tttcatattt aattagcctc cgaatcgata ttttctggtc ggcttgctgt tcttggcata
    43261 gcgttaattt gggggttctg agtagcaaga taagcagcta tactttccat cttctctttc
    43321 tgttctgaag ttcttgcaag ttctttaatc aattctttgt tttcagccat caagttttga
    43381 ttggattcat tatgactctt gattacctcc atattacttt ttataacatt cgtgtggttg
    43441 ttgctaattg ctttaatcgt tctgtgtgta aaccatccaa taagtaaagc agctaaccat
    43501 ccagaacaaa cgagcaacca ttgtgaaata tcttggttgc ggatgaagct agtagcaata
    43561 gtgattatgg tcccaacaac gccaattaat ccatatacat gattcagcat cctttaactc
    43621 tctcatgcca cgaattctta aggatcttgc gacactgaca agaaccacct tacatcaatc
    43681 tattttttgc atatttttca ggatagtgcc atcaaaaaag acagttttgg accataggcc
    43741 atagattttc ttcattatgc tccaatgtat taacgaggta tgtactgaag gcactttgac
    43801 tacacaaata attaactaac ttgtcggttt gcgcgcggtg ctttccccgc ctcgcccgcc
    43861 cgctttgcgg agcggtttta atgcagttgc actgacacgc tcaggccgtg ccgggcgtgg
    43921 cctggtatac agaaaatgag acgtgaaaac gcatgcaaag ccatgcacct tatcgatgca
    43981 tggctttttt cagtaaaaac gggcggattt tcggggaatt ttacacagac tgacgtgatg
    44041 ccagttgcgc acttttacgc gaaaaaatca tgttctgcgc aggagtgaat ttttcacggc
    44101 tgtcatccac cgaagccacg tcaggcctga atccgatggc cgttaaaatg tcgctatcct
    44161 gtacggaata attaattttt tcacccttcg caagccagga cagaagggct tcacgcaggg
    44221 catctgtggc acgctgtatg gcacagtttc gggcaatggc cgtcagctca ctgtagccca
    44281 tcagctccgg tgccagtgcc gccgccagtg ctgtgccgtg ctgctgcata aaatcattca
    44341 gccggtcgcg gatgctgatg tgctgaacgg cttcatgcga acgaatataa cgaccggcgg
    44401 cctgattcac ctgccatttt ctgacttcga taatattgcg taattcgtcc aggcgactga
    44461 cgtttctgcc ttctcctgac agaagccgca ggtattcctg ttcggccgct gccagctcat
    44521 ttttgcgttg cagccatgct gctttgttat tctgacaggt gtcaaaggcc tgctgtaagg
    44581 ctgtgctttc catcgttatc tctttctcat catgctgaag aataaaaata cggtgtgcgg
    44641 cgacggccgg tgttaaccgg cagccctcat tccagacgca gcgaatatga ttgtgttttt
    44701 aagcgtactg gcggcagttc ctgtttttca tgcaggcttt ctgccagttc gtccggcgtg
    44761 accgggcgga caatgaaacg gttgatggtc tgaagcgttt taaacaccag accacagccc
    44821 ggatccgtgc acacataaaa acgctcggtg acttcctgag acagacgccg cgatgttctt
    44881 gacagtgcaa ggcctttaca tctcctacaa caatatccgg taacaagcat tcttttcggg
    44941 cgtttcatgc taccggaggc tgacgtcagt gaatcgcggt atttctgttt gcctgaaatg
    45001 tattccattc ctgaatcttt acagtcagag aaaaagcttt cactcgcttc aaatgtcgca
    45061 gagcaataaa tattccggca ctgtgcaatc attatcttgg tgccatcgtc catgaaatgt
    45121 gcgcggcggg tgtgagcaac atgtccacac gacgggcagt aaatcatgac agcagtcctc
    45181 tggccttaag ctctgcaccc tgctggtcta ttttgtcctg ccacaccttg cgctgtgccg
    45241 gtgtgcctgc cacctcataa tccatgtgcg ggagtgttgc cgctgacagt ccggtcagcc
    45301 ggagaaccgg ctcgccggtg aggctgattt gcatctgttt aattttctgt tccagcgatg
    45361 atttcacctg ctgcatgaca gctttatcgg gtgcgacgta gccctgatgg ccggtggtgt
    45421 tggcgagcgg attttcctgt accaggatac tcagatgcat tgcccggaca agcgcctcac
    45481 aggtttcatt cagggcgtgt tccagctcat gctctgcata cagactcaga aggtgatgat
    45541 gtgccttccg gtaggcggtg gccgtgctgt cacacgcccc tttcaggcgt tcacgttcga
    45601 aattcagcac cacggccaga ttgtcatatt cctgtaccag ctcccggcgt gccacgcgct
    45661 caatgtggcg ctgtttcagc tcgtcgctca ggacaccacc ggctgcacga aaggccgtgc
    45721 gccagtcgtc agcgtcgttt ccgtcggcct gcgccagcgc atttttttcc tgctctgccc
    45781 gttcaatggc cgtgacggtc tcatccatca ggcgggcgtt ctcaaggtgg gcggctctgg
    45841 ccttttccag ttgtgccagt gcgggttgca gatattcagg gatggtgttg tcagacattt
    45901 tccggctcct cgtcacttca ggttaagaaa attgtgacgt acaccggaca acaacacgac
    45961 gcattgcaga tgtgccagcc ctgacacagg agactcatcc tcagaccggc aagccaggaa
    46021 aaggtcgcag gaaaaaccgg cttactgttt gtttttttat attttactgt tcacctctgt
    46081 tcatcataag aaaaaagata agtaatacag taagttaaag ggtgaacaat cgcagtaatg
    46141 actgttcacc gtctgttcac cactgttcac ccgttgatgg gctttttgtg ctgtttacta
    46201 ctgtttgttt ttattaattt gccgggaatg aataagaaaa aacgatttgt atttcactat
    46261 aaaaattacg aattgcttta agtggcttta aatgacttta agtggagatg aacaaaaaac
    46321 acacaagcat tgtaaggctg ccggaacaaa tcccccctgt tgcgtctgct gaaaatattc
    46381 acaaaataaa gcgctacccg aagccggacg gacttatccg gtgctgtatg gacattaacg
    46441 aggtagcccg atgcaagctg ttttttcttc cccgtctccc gcccctgtga cgccactgat
    46501 gccgctgccg gacatcacac aggagcgttt tttacgtctt ccggaagtga tgcacctgtg
    46561 cggcctgtca cgctcgacca tctatgaact catccgtaag ggggaatttc cgccgcaggt
    46621 gagtcttggc ggtaaaaatg tggcctggct gcactctgaa gtcaccgcat ggatggccgg
    46681 gcgcattgcc ggacgcaaac gggggtacga cgcatgatga tgcccgctct gcaaaaactc
    46741 cctttttctg gcttgccttt ttccggcatt tgcggatata gtttttccgc tgccgcaaaa
    46801 tcggcagccg ggcgtgagaa cccgagcaat tcacaggcga catatgacgc gccacgcgtc
    46861 tttttttgtg tcgcaatcaa tgccacagag cgccagatta tggtgtggtg tgtggtcagt
    46921 cgtgcagatg gcattccgtg catcctgccc gcgtccgctc attatgccgc agagtcaatg
    46981 gtagctcagg cggggcagcc tccgggctgg ccggtatcct gtgaagccgg tattctcacc
    47041 cccgtctggg ctatcgccat cgagcgtgag aactccggcg atagcgttat ttgctattca
    47101 caggaggctg ccatcatggc tacaaccctt accccctcac accctgaatt tgtctttgtg
    47161 tttgcggctg tccgtcgcgc agaccgtcat ccccgtatct gcatgcttcg caccgtcgcc
    47221 ggtgatgaac gcagcgcccg ccgttccctt gtccgtgact atgtgctctc ccttgctgcc
    47281 cgtctgccgg tggtggaggt gtcccgtgcg taataaaaaa gcccctcaga ccgtctcagc
    47341 gcgtcatgac gcccgtgaac acctcagcat tgaggcttac cataagctca accgcgccag
    47401 cgccgtatcc cagtttgttg ggggtgattt gattcaccgt gaactctccg gcctgcatca
    47461 gctctacatt ccgcatattt tcagctacct gaatgaagat attgattttg tgctgaatga
    47521 gctgaaagcc aaaggcctgt gccgcgattt tctcgcccag cagaaagacc ggggagacag
    47581 gacgcatgtt tgattttccc cagcccggtg agatttaccg ttctgccggt tttcccgatg
    47641 tggccgtggt cggcattctg gaagacggta ttccgtggga aatgccgtac cgctgcccgg
    47701 acattgtctg gaacccgtac cgccgtaaat tcagtatcct tgtgcgtatc ctcgctgacg
    47761 gacgcaccac agacatcccg ctggggcgtt ttctgcggga atttacctgt gaccgtcctg
    47821 acctgtttaa acgcagcccc gtaaaccggc atgcggtact gaaagaaatg gccggagacc
    47881 cggaattaca gaaatggcgg gaaaaatatc tggatattta cccgcaggac actgttccgg
    47941 tcagccgggc agcaccggtg gcgcgggaat ggcgggaaat tccccgcacg gagcctgacc
    48001 cggaaaccac cccggataac agttaccgca attatctgta attaaaaaac gacacccgaa
    48061 aaattaaatg tgcgtattcg cgcagggata cgcacgtctt caggagacgc agatatgcct
    48121 tatcagttaa tgcaaccggc acggaatgca gtcatctgtc acagggagga aagcaaatga
    48181 aaacaccctt accgcccgtc ttacgcgcag ccctttaccg tcgtgctgtc gcctgtgcct
    48241 ggctgaccgt gtgcgaacgt cagcaccgct acccgcatct cacccttgaa tcactggagg
    48301 cggccatcgc cgctgagctg gaaggctttt atctgcgcca gcacggtgag gaaaaagggc
    48361 gtcagatagc ctgtgccctg ctggaagatt taatggaatc cggccccctg aaggccgcgc
    48421 cgtcgctgtc ctttctcggg ctggttgtga tggatgaact ctgtgcccgt cacataaaag
    48481 cgccggtact gcactgaagg agaacaacac catgaaaatg aacgtaaccg ccaccgtcag
    48541 ccatgcgctc ggccactggc cgcgtattct cccggcgctg gggattcagg tgctgaaaaa
    48601 ccgtcatcag ccctgtccgg tctgtggcgg gagtgaccgc ttccgttttg atgacaggga
    48661 ggggcgcggc acctggtact gcaatcagtg tggtgccggt gacggcctga aactggttga
    48721 aaaggtgttt ggtgtctccc cgtccgacgc ggccgcaaag gtggctgccg tgaccggcag
    48781 cctgcccccg gctgacccgg cagtgacggc cgccgccggt gctgaaacag acgctgcccg
    48841 gaagaacgcc gccgcactgg cacaaaccct gatagcgaaa acccgtcccg gaaccggtaa
    48901 cgcctacctg acccgcaagg gctttcccgg ccgggaatgc cggatgctga ccggcacaca
    48961 cagagccggt ggcgtgagct ggcgcgccgg tgaccttgtg gtgccactgt atgacgacag
    49021 cggcgaactg gttaaccttc agttaatcag tgctgacggc cgtaagcgca ccctgaaagg
    49081 cgggcaggtc aggggcacct gtcacatcct tgaaggacag aatcaggccg gaaaacgtct
    49141 gtggatagcg gagggatacg cgaccgcact taccgtacat cacctgaccg gtgaaacggt
    49201 gatggtggcg ctttcttccg tgaacctcct ttctctggcc agccttgccc ggcagaagca
    49261 tccggcctgt cagattgtcc ttgccgcaga ccgtgacctc agcggtgacg gccagaaaaa
    49321 agccgccgca gccgcagatg cgtgtgaagg tgttgttgcc ctgccgccgg tcttcggtga
    49381 ctggaatgat gccttcacgc agtacggcgg ggaagccacc cgtaaggcca tttatgatgc
    49441 catccggcca ccggctgaaa gcccgttcga caccatgagc gaagcggagt tttccgccat
    49501 gagtaccagc gaaaaggcca tgcgtatcta tgagcattac ggcgaggcgc tcgcggtcga
    49561 tgccaacggc cagcttctgt cccgctatga aaatggtgtc tggaaggtgc tgccgccaca
    49621 ggactttgcc cgggatgtgg ccgggctgtt tcagcgtctg cgcgcgccgt tctcctccgg
    49681 gaaggtggcc tccgtggtgg acaccctgaa gctgattatt ccgcagcagg aagccccctc
    49741 ccgccgcctg attggctttc gtaacggcgt actcgacacg cagaacggta cgttccaccc
    49801 gcacagtccg tcacactgga tgcgcaccct gtgcgatgtg gatttcaccc cgccggtgga
    49861 cggtgaaacg ctggaaaccc acgctcccgc gttctggcgc tggcttgacc gtgctgccgg
    49921 tggtcgtgcg gaaaaacgcg acgtgattct ggccgcactg tttatggtgc tggcaaaccg
    49981 ctacgactgg cagctctttc tggaggtgac cggtcccggc ggcagcggca aaagtatcat
    50041 ggccgaaata gccaccctgc tggctgggga ggataacgcc acgtcggcca ccatcgagac
    50101 gctggaatcc ccgcgtgaac gtgccgcgtt aactggcttc tcactgatac gcctgccgga
    50161 ccaggaaaaa tggagcggcg acggtgccgg actcaaggcc atcaccggcg gcgatgcggt
    50221 gtccgttgac ccgaaatacc gggatgcgta ctccacgcat atcccggcgg tgattctggc
    50281 cgtgaacaat aacccgatgc gcttcaccga ccgcagcggc ggcgtgtcac gccggcgggt
    50341 gattattcac ttcccggaac agatagcccc gcaggagcgc gacccgcagc ttaaggacaa
    50401 aatcacccgc gagctggcgg tcatcgtgcg tcacctgatg cagaaattca gcgacccgat
    50461 gctcgcccgg tcactgcttc agtcccagca gaactcagac gaggcactga acatcaaacg
    50521 ggataccgac ccgacgtttg attttatcgg ctatctggaa accctgccgc agaccagcgg
    50581 catgtatatg gggaacgcca gtatcatccc gcgtaattac cgtaaatacc tctatcacgc
    50641 ctatctggcc tacatggagg caaacggcta ccggaatgta ctcagtctga aaatgttcgg
    50701 gctggggcta ccggtgatgc tgaaggaata cggactgaat tacgagaagc gccataccaa
    50761 acaggggata cagaccaacc tgacactgaa agaggaaagc tacggcgact ggctgccaaa
    50821 atgtgacgac cctgcgacaa cctgaccatc atgactcatc tgaccggcat ctgccggtct
    50881 tttttttatc cctgaattcc ccgcaggtga acaatccact gttcatcctt caccgtgtat
    50941 tcacccgtta tcacactgaa attaaaagag aaaaacgaaa ggtgaacagt gtgaacaatc
    51001 aaatcaaaaa aaactttttt cgccctgtgt gatttcagtg cggaggatta accccctgca
    51061 caagtcacac cggcagaatg ccggaggtga agaatcgaat gttcaccctt cacccattat
    51121 tcaccaccta tcactctgaa ataaaaggag aaaacagaaa ggtgaacagt gtgaacagtt
    51181 ctttcgaaaa aaaattttta tgtggataaa agatgcactg gtttggatcc gagctacggt
    51241 tccaaactta ctgattaaaa atgatttaac tttccgattt attgatgggc tgaaaataga
    51301 ttgctcagaa aaacagcggg ggcacaaaag ggggcatatt tcattgttgt gttttattat
    51361 ttgtttttta tcattaactt attttgatgt ttgagtcctg ccttcgcacc acatcgtatg
    51421 caaatcgagt cgctcaaggg cggctttttt tgtgcctgaa atccagtatc cgcaaggctt
    51481 tcctcgcttt ttcactcaac ctaagtcaac ctgattcaat ctacatcaac ttactgatgc
    51541 gggtacaact gcgggtattt cccggttcga taatgtttgt acccacacag aacctttgaa
    51601 aggacactca tcatggctct gagttatgtg aaggttcgtg ctgcccagcc tgaaataaaa
    51661 gtctataagc tggctgatga cgaaggcatg gtgttgctgg ttcaccctaa cggttcaaaa
    51721 tactggaggc tacgttaccg ctttggtggc aaagagaaga tactggcgct ggggaaatac
    51781 tccgaagtct cactggcgga tgccagggca cgcagggatg aggcccgtaa gctattagct
    51841 atttggaaag tggcaccaaa atatttcccc atcatgaatt cgtaaaataa atgagaataa
    51901 tcatgaactt aatgattcca tgatggatag cgacaacgat agctttagaa agtagtacga
    51961 cgctactgtt gcataaattc tctatatcct gttatagtct tttcactgac ctagtacaac
    52021 gctactacct aggtaaggga gggagtaaat agcagttgcc gctgctattt actcaacaca
    52081 ttctggctaa aaccgttacg agaggttcta acaaaatgaa cgagaaaaaa tatcaatccc
    52141 tgcgtaaaaa gtcaataagt aaggggggga tggcttgtat cgtcatgctg atacacacgc
    52201 tctccttttc ctcattacag atggatttag atgtacgtgt gggggatgtg aattggaaac
    52261 actcgattgt gcttaaaact actggaggaa agggagagta atctccctaa cttacttatg
    52321 tataaaattg ttacattaga gagtgttgct gagatccgct taggtatgcc ctttaaatcg
    52381 gcgatacaag atgctggtga gcaaggctcg tgttatctta tccagacgaa agatatagga
    52441 cttgacggca ttttagatct tggtacgtta acctctgtta tcccagaggg taatcctgag
    52501 aagcattatc ttttccctaa tgatatttta ttacgtctgc ggggacctgt gttttctgct
    52561 gggattattg aaggaaatct aggcaaacca atcattacgt ccaatcagtt agccgtgatt
    52621 agatgtaatg aaaatctaat actaccacat tatttacatt ggtatataaa ctctacttct
    52681 gggagtaaat atattcgcag tttgagtgaa ggtactagta ttagtaaaat taattcaaaa
    52741 acggtttcta aaatggctgt taaactcccc aatttgagag ttcaagaaaa gattggtttt
    52801 attaatagaa actggattaa acaaaaagtc atctataatt cactaataca aaatggcgat
    52861 gtgctctttg atggtatatg tgaaaatatc attgatagcg aggagttaga aaatgagtga
    52921 aatgaaggca cttcataaat cattgttgct tgcttgtgat ttatttagag gaaaagttga
    52981 ctcatcttct tataaagatt atatattttc aatgctattg ttgaaatata tttctgacat
    53041 aaaggctgac tatttatctg atttaattaa tgaagactat tctgagttgt tagaaattgt
    53101 ttctcgagta cctgaggatg caagttttta taaaatatat catgaagcta agaatcatgg
    53161 tgattatatt ggagagagga ttgacaactc gctgagatta attgatcagg ctattgattc
    53221 ggtctgtcaa aatagaggtg gttatttatt cgagtctata caatttgcaa cggttaattt
    53281 tggtgcaaga tcagaaagag atcggatgct taataattta ctcgctattt ttgcacgtcc
    53341 tgaaatgaat tttggatata ctcatgatgg taatgataag ataaaagtgg cgtgtagata
    53401 tctttttgaa aggattgcat ctgactcggc gatgaggggg gcagagtttt ataccccaga
    53461 aggtatttca ttactatttg cagagttatt gcagcctaag ggtggagata gtatttgtga
    53521 tcctacttgt gggtctggtt ctcttttaat taccctaggg gaaaagataa aaaaatcctt
    53581 taaaagcaat aattatactt tgtttggtca agaggttaat aggtccagtt gggcattggc
    53641 taaaatgaat atgattatgc ataacgaaat taatagtcgg atagaatggg gggatataat
    53701 atctagccct cagtttctgg atacagataa tagattaatg aagtttgatg tggttgcttc
    53761 taaccctcca tttaatatta tcggctggaa tcatgatgat ttagtacata atgattatgg
    53821 tcgttttaat ttaggttttc cacctcaggc caagggggat tatgccttta ttttgcacat
    53881 ggtttcaaca cttaaaagtg caactggtag aatggccata ctggtatctc acggtgtatt
    53941 gtttcgtgga ggtcaagagg agaatataag gagaaattta gttagtgaag ggttgttaga
    54001 tgcggtaata ggtctaccag ataggttgct ttttgggaca gggataccaa ccgccatttt
    54061 gatcttcagg aaagataaaa agaattctaa tgtcgtgttt atcgacgcat ctgatttggc
    54121 taaacccgta aaggggcgca acttaataac agatgaaatc attaggaagg tatcagaatg
    54181 ctactttgaa agaagtagtg tctgcaattt ttcgcatgtg gcttcttttg atgaaataca
    54241 aaaaaatgat tttaatttaa atatttctcg gtatgttaag aaacatgaag agccggcttt
    54301 tgttgatctt aagggcttac gccaacagcg agaagaacta ctttcaacat tgtatgaatt
    54361 agaaagagaa atgaaaggtt ttattgataa ttaatggaaa tggttgatgc gatagtatca
    54421 accattttct gattaaattt caatacttcc agtgattata agctaatggc attttattca
    54481 acttagctct gtgtccgcgc caatttggtt catcatagcg gtaaaacgct cgttatgctt
    54541 gcattcgtgt agatggacga gttcgtgaac tacaataaat tcaaggcatt caggtggttt
    54601 tttaccagtt cgagattgat ccagatgcgt gcggtctgtg gattacagcc accccatttg
    54661 gtcttcattc gcttgatgcc aacaaaactg gcttcagcct ttacctcttg ctgctatttt
    54721 tccattaact taggcagtct ttgttttaaa ataccgcaat accactggct gagaactatc
    54781 ttccgttaag ccgtgtcgtt atgtggggga actttcaagc gtatccagcc tccctgaagt
    54841 ttcacttaag ccggtttatt cactaaaact acatccagca gatatcctct tccccaaaga
    54901 tagtgtgttt cgccactaca tatcgagagt gaacgcttct atgaatcgcc cgtagaagag
    54961 gaacgcctga actgtgagtc catacgtatg ggcgcttttc tattgggaag cgatatagcg
    55021 acaatgagtg agcactcatc cctggaaggt ttactgagtc ttgataaaga acgcctgatg
    55081 gatgtgttag tacgacacta tgatgtgggt gatgccggga ttgagcagat catcaatgat
    55141 ttctgggatg gtatggttag cgatcgttga ggagagcttt gaatgaactg ttcataatac
    55201 tattctgttc atgaattatg aaccgtgtca tcgtgaatat gtatggatat tatgtcgaaa
    55261 aattgatttt ttaaacatat tttccagacc gatttaacca tttaatcagc ctcattcctg
    55321 ttacagagct acctgtaaaa tggcgcacca tgcaatctgg caagggagta ttgcgactac
    55381 tttatgccat tgcagattat catcctgatc ctggtggcgg cgttgactct ggggttggtg
    55441 ctaattgtgc tttggcggcg catatttggt tatcgcagct ggtgatcacg gtaaatatgt
    55501 catattaatt gtagagcatt tgagatggag gatggctgtg taggatgagt accttggcga
    55561 tgtttatgca gcgatatcag ttgctatctg gattattata aaggtcttag aataaacact
    55621 ggctggtcag ggaaagacga gaatgactga atattggtgg aaagatcttt tcgatcggaa
    55681 taacaactgg caggggctgg agtttacgat tcgggcgcat cagggggttg cacaggcaat
    55741 ggatatgctc agtggtcatc atgggcggat ggcgttgcaa ttacatggtg agacattatt
    55801 ttgggcatca atgttaaaag atcattcagg tgtctggttg gtgtttaacg ttgatcattc
    55861 tggacggcag agtttagtgc cccctgtgac atcagcagat gtagaacaaa tcaaatgcaa
    55921 aggggctgag gtttggacgg gcgagtggtg tcggtatttt acccggcagc taatgaatac
    55981 tccagtaccc cttctgcctc cgcgtcgctg gttgctaagg cctgtggata ccaccagacc
    56041 ctgtaaccaa tccatttcta tagagaactg gcgctttgat tctcctgaga gcaccgggaa
    56101 tatcggctgt gactggacgc tttatggtga agatatcccg gatctgacta atcctgaaaa
    56161 aatcaggcta gtggactggt ggtggggagg taatttgtta cttggacgtt accacgttca
    56221 tttcaacgca gggcgattga aatggtggcg taaaaagtgc cgcgaggaaa cattgccccc
    56281 gatactggtc tggtacattg ccgggttggg atcatttgtt attctggatg gtcattatcg
    56341 ccttcaggct gcgattgaag aaggtactcc cccctcattt ctggtactca gtgaacttaa
    56401 tgaacggaat tttcctgtcg accaggaaca ccaggcgcgt attactcggg cgctggagcg
    56461 gcagagtcag aatccaggtt gtagcattga tggtgtaaat caaacactga ttaatcttta
    56521 tgataaccgt tacctgtacg catcgactta cagccgcgct attctcggta aaggtgaatc
    56581 ttgggcggca gatgtaaata gttatttgca caagcataat ctggaagaat atctggataa
    56641 gatagtaaat cgggttaatg attgatgaat cagagcggag gttctagtga aggatttttt
    56701 cagggagtat agagatacca ttcttgcatc tgtgggtatc atcgtcgctg tattttcgct
    56761 ctactttact caggagcaaa gtaaggaaca gattaagcat aatcatatct ctgtcgaacc
    56821 acgtataaac gcttatgtct ctaatgatgg tcggataaat aaatcaggcc tttacatcat
    56881 aaataatgga atggggacgg cttttgtctc aaatattacc gtaatggttg atggtaaaaa
    56941 gattgatcct gtgggagaca acatctttct tgcttcagtg catgcgctcg gattggatat
    57001 tacgtgtttt gtgatcggag gtccaagacc aggtgattca ttcagagtgg gcgatgaggt
    57061 gttcctggtt gaggctcggg gaacggatag tcgctgtgat gtcgattcac ttgagttgcg
    57121 attagctgcc tcccaaaccg gcaggctgga tttcattctg gacgttgagt ccatttatgg
    57181 tgacaaattc cgctatattt attcgcagaa taagcaagtc aagctgtaat caaaagggaa
    57241 gagatcggcg ccatttgagc cgtcaggccg ttgatctcac catattaatc tttattcgtt
    57301 ccaatgagtt gtgcgttggt cagaaattga ttttgaaacg gcaatgtgga cgataccggc
    57361 tgaatgagaa ggtattgaag gagtgaagta ttcgccgcgt ggctcgaaaa tgcgtacacc
    57421 tcatcgggca ccctaactca acaggcacag tgacacagaa ttcgaaacgt tctcgtgacg
    57481 tttcgattaa taaattctaa tgttgtataa aataattcat taactcgtgt tctatatgct
    57541 caacggccat atctatacgt ttggcaatat tattatcaac atttaaattt aaatgactac
    57601 ggaacctaag tactgattct gcaatgttat caacatttcg ccagtaaaga aatttggaca
    57661 tttctgattc tctaatggaa tgcaatgctt tcatagtgga gccatcatga taaacatgat
    57721 gaatatcttg agcaatgaaa tgtaatttat ggtcagttgt accaacccaa tgttcgatat
    57781 taaattttgt gccatttaaa tcaattgtgg cgccaatgtc ttctgagaaa ttcgcacctg
    57841 ttaaccaatg caaatccatt gctgcatcaa aagcattcca gcgacattta tcaatattgc
    57901 ttccattttt ataaaaattt tgtttaatgt attttcgacg tttgtggata ttttctggta
    57961 gctgattgat tgtattagca tcaaggtccc agaaagcata tttggcaatt tccaggtcgt
    58021 aggcactgat caagtcaata aagcttatca tactataaag ataggtttcg aacttatctt
    58081 caggggagag gtgagagtaa ttaagcttta cgttttgaat ttgcagcatt gagatataat
    58141 gcatcccata cactgttctt gcttcatctg ttagtttttt aaaatcaacc ttggggttct
    58201 cagaatcagg attataatct ctgaaagtcg cttcaggatc atcaatatgg ccaggccaga
    58261 agagatggca gaaattctgg tatgcagcca ttgatttctc aacccagata tctggcatct
    58321 cattgacacc gatggcagtc aaaagtactg tccctgattg taaaagctca actaagggca
    58381 acaatccaac tcgacgaaca tcttccagtg ttgaattcgt tgatagctcc tccattctct
    58441 gtaaagaaca gttatccagg ttgatttgaa cggctctctt tttattcaag ctctgccaac
    58501 aatactcaga tgtaagtatt tttttatctt catgaatgga gaggcaaggc ctgatgaatt
    58561 taattacaga acgttcttga gccattgtcc tctccagttc attaaaatag ttactacgtt
    58621 aattcacacc cagcgcctta taaaccgcat caaccgggtc tgagtaaaaa ctcacctgaa
    58681 acttggtaaa taactctgcc ggaaccgttg gaatatccat agccgaggac atcggcaaca
    58741 gaacccgttt tgcgccgctg tcgaaggcga gctgtaaact ggcggcaaga tcctgtaccg
    58801 ggttaattac tccaccaagc gtcatactgc ccaacaccac catctgttcc tgtactggtt
    58861 ttgccagcaa tatcgaacat aaagcgataa gcgcagcaag actggttgcg gtgcttgggc
    58921 cagtattatg cagttcaacg acatgaaggt gatattcatg atcggagaat ttcgcggccg
    58981 cgcttacccg attcaaattg cctttgaagt aatcgaaacc gacgcggata gcttctttcg
    59041 cggaggtatt tgaacccaga cccgatacac tatgcttacc attaccggca gtcatctgtg
    59101 tttcaaaacg atacaacccg gtcatgccgc tttctgcctg agtgaccaga tgcacaacgc
    59161 ccggctttgg cattccggca ggaataagtt cgctgccgcc ctgttccggt acgctaacaa
    59221 aaaactcttc cagcgtttcg ttatcgatgt agctaaagtt cacatcgaag aactccaggc
    59281 cgcccagttt tttaagttgc tctttcacgc ggcgacgaac ttccatcgca taggtcaggc
    59341 agactcgcac atcttctttg ctgtacgcgc catcgggatg catgagtttt aacaatcccg
    59401 acacggtacg tcgaacggca atgacgtcac gctggttgag gttgttaccc agcttaaaga
    59461 atttatcaat cgcatcagag aaactgcgtt tgcgcatttc gcgcatatat tcagcgagat
    59521 aatccgtaat cagcccgtaa cggttggtaa agaattccgg gcgcattttg gggatttccc
    59581 aaccgggaat ataggcatga aagcggtcga aaaatgcggt atcaatcatc gcagccggaa
    59641 acggcgccag caaatggctg gttttaacta gcgtctctac gctttgattg atgttgccga
    59701 cgaaaaccat cgacgcttta ccttcaatcg aatctctgcc gcgagagaaa gatcctgacg
    59761 ccatgtaatc tttcatgatt tgcacgccgt ctttatcttt gaaagtgatc cccgcgactt
    59821 cgtcgaacgc taccacatcc cacataccaa ccaggccgat ctggcgactg gccatgttgt
    59881 agaacaggtt ggcaacggtc gtttgcccgc cagaaactaa cagggagtta ggagaacact
    59941 ctttatacac atggctttta ccggtgccgc gcggccccag ttcgcaaacg ttatagttgt
    60001 tctccacgaa cgggatcata cgggtgataa ggtgccattt ggtgcgttgc tcaatattgg
    60061 cgggctccat acccactgag cgcagcagca catcgatcca ctgatcacgg ttaaagtgtt
    60121 tacgcgcatc gaacacttct tccatatcca tattcggcat ctggataggc ttaagcgtca
    60181 tcaatgagaa gggcgaagtc ttctgccctt cctcaaagaa atagttgacg gtaatcatgc
    60241 accagatacc gcccgtcagt agcttctcgt tgtctttaac catctgcgat ggcaccagcg
    60301 cgtctttgat gccgaggtta gaaagctggg cttcgtaaac gtcttttttc tggtttagct
    60361 tcaccgacac tttatcgatg attttgtacg aaccacgctc gcggatcagc gacttcactt
    60421 tctctgcttc atccgggcgc acatagttat cagccagaat acgcttaacg ttttgcaacc
    60481 cttgctcgac cacgtcgtca tcgtcagagg cgcagtacat gccgagcaga tactcaagca
    60541 catacaccgg gacgtttgcc ccttccttga gttgcttggt gagatcttta cgcaccacac
    60601 gaccagcaaa atgctggttc agcagggcgt ccagatcgta accctcggag gcaatttccc
    60661 ctgcggatac gcctgaaaca ggtaaatcat gatgggtttg catatcgcct cacttagaag
    60721 aaatcatcct ggaacgccag atcgataatg accgggtact tctgataccc cgtttccgtt
    60781 tgtgcgtctt ccagtatcaa ccagtactca ttgcgacggt tgaagttagc gccaatcagc
    60841 ttcagggtaa cttcgcgcac gcgtttttcc atggtgctgt tatcgctgtc aaagctgatg
    60901 cgctctttgc ccgacaccac attattagcg ttatcgacaa tgtaaatgtt caggatacgc
    60961 ggttcataaa gttcgcccac tggatgcgtc tgcaacaggc tcactttatc gatattgttc
    61021 accagcttaa tcatgggatg gtaagcgaca atatccaccg ggcggcgctg cggctgtttt
    61081 tctgcggcgg ttttttgcag ggcttttatc tgcaataccg gaacgcaaac ctcctgcaac
    61141 atggtgccgc catgaacaaa gcgcgcgccg ccagagaaat ggaagcgctg gatccctttc
    61201 ggaatcagga actcgctgtt gtcgctcaca cctgcggtat ctgccacttt acctttccag
    61261 caaaaatcat cggcgggaag ctgatggccg ataataaagc gtttgtggtt cttgatagtg
    61321 ttttccggct taatttgcag agtggtttta tcctgaaccg aaagcgcctg ttgctggaac
    61381 aggaaaccgt gatccgccgt aacaaaaatg cgtgtgccgt ggagacggtt gattaccttg
    61441 gtgactaaat ctttcagttc agtcaccgcc gtgcggcacg cttcgaaggt tttatcttcc
    61501 gtggatgccg tgtcgccggt ggcatcaatc gtgttatgcc agatatacac cacttcgtaa
    61561 tcgcgaataa ggtctcgtcc ttcctgattt tttaactcca gaaggtcctt cgattttatc
    61621 gccattccct tatagttctt cagaatggta tcgcggttag gaatacccga ggtcgacagc
    61681 ccatcagcat aaacgatgtc gccgttaccg ggttgatagc aaagttgttc atggggcagc
    61741 aatgccgcca ttcccagttg ggtgtagctg gggagcacgc cgagctgcga gcgcagttct
    61801 gcggtaaagc gtttctcggt attgatttga ttccccagct cctccgccac ttcataacgc
    61861 agggcatcgg aaattatcac gaacacgcgt ttgatttgcg gattttggaa ctgtggcttc
    61921 accacctcat tgaagaagtt ctgctgacgc ggcacgccgg ggatttgcca cgcctgcata
    61981 cgattttccg cttccagcac ttcgttccag ttacggctta attctgccag ataccagttg
    62041 ctgtagagcg cctcgatata atcatccagg ctcttgagga tcatcgcccc tttgctgtga
    62101 accagcaggg catattcatt aaacaggcga taagcctggt cgaagcggaa cagttcttcg
    62161 cagtaggctt tccagaaggt ggcgctgtcc tggtagtgga aaccatcgat gtggcgattg
    62221 cgcagattca gcaaccgctc tgcctggcgc agtgcgtcat agatggcgta atactctggt
    62281 tgtgtctgac accagtattt actctggcgt tcagagagca attttttaaa ggcttcccga
    62341 tcgagcgtgg tgctctcttc cagcagctgc gttaccagcg catgaataat ggtttgttcg
    62401 atgctgaggg tggtttcgca ttcgtgcaaa tcatacggcg agctgagccg gtaatgatct
    62461 tccgggcgca gggcggcctg catttgctga gcgcagtagt cataagcgtc tttatagcga
    62521 cgatcggcac gccaggtcac cataaatgcc agggcggatg ctctgccgga tggcgtcagc
    62581 aggacatttt tttccagcca ggcgcgctgc tgtgggtcgg cctgggcaga gagatcggta
    62641 caaaacagtt tcaggagcag gttttccagc gatggctcct ctgcctggta gcccatttcg
    62701 tggttgagca tttcccacaa taccgagtcc agacagtggc gtttcagcat cgcctgcgtg
    62761 ttttccagtt cgctgtcgtc ttctgtttgt tggttaacgt actgggtaat cagattgaac
    62821 aaaatgtctt cggttttcgc ggtctttgcg ccagcgatca ccgcaatcat tttcttatcc
    62881 agcgaggctt catcttcctg ttctgtcacc aggtttttca gcgcctgcgt gcgtttagtg
    62941 ctgaagaagg ccttgcgtcg ctgaatatgc tcgcgcaggc caagctgcgg aatgcccagc
    63001 gtgttcaggg tgatggcggc aaaatcggca tggaattcgc tgctgtaaag gcggatatcc
    63061 agcagccagt cttgttcatg aggcggcgca tcatgaggaa accacagcag gaactgctgt
    63121 tctggctcat caatctcgat gcgttttttc accgacagct gtgacgcgtg ggtcatgttg
    63181 attagcgtga cgttttcaag ctcgagctgt tccagttcct caataaaacg tttatcggga
    63241 tcgtgccaga aaacgatgcg atgttcggca aatttagctt taaggccagc aataaagtcc
    63301 tgattttgca agttcacaat cccatgttgt tacgatgaat aagaaagcgc caaaacgcga
    63361 aaaggagatt ttgacacggt ggcgcatttt atcatgaagt gaggtggaga aatgccagca
    63421 gcggcgcgtt tcccggtggt atttgccacc gggcagaacc cggcggcagg ggaacgcgcc
    63481 atctggttta gatcacctct ggggcattgc cggtgatggc tttgacatct gccagcagat
    63541 cgccaaactt gccgtagtta accttaacgc catcgtcgag atcaatactg attctcatat
    63601 cagcatagtg acgcaggcgg tcgtcatagc tacgcagttc gctgaatttt ttgatcaggc
    63661 tgtcgcgttc gcgtttcagc cgtgtggctt caccgccaga agcctcatca agttgatcgt
    63721 tcaggcgatc gatattggcc tgataacgcg ccagcagcgg tacaacatat tcggtacgca
    63781 tcctcgacag cgtggcgtcg ttatagcgat gcagatacac caggcactcg aaggctttct
    63841 ctttaccgga gctgaacagc cagtagattg gacgcttttt atacatcttc agatgatctt
    63901 tccagaactg ggtggaaaga tagcgacgaa tggtctcaag cgcagattcg ccttttttcg
    63961 gcttgatcgc gtataaacat agactttcgg cgataaattc gaggttttcc tgcaggtgct
    64021 cttcgcccca gacggtgcgg acaaactctt tgacgcgaga ggtgacgtcg tcctcaaacc
    64081 actcgtcatc catcagcggc aggatgccgt cgctgtcagc tgggaaggtt ttgtaggcac
    64141 cttcggcgac gagttcggag aaacctttgt taccttcatg ggcgtagaca aggccttcgc
    64201 gatcgaggga gtagcggccc atttggcagc cgatggaata tgatattagt tctgatatca
    64261 gtatagtaac atacagcgat tctttgtttg cagtatcaga aaaaatataa gccatattct
    64321 taaataaggt tatttgagct aaatccactg tttggttaac ataactctct agatcataag
    64381 taatgttaat ttggtgattt aattccgatt caattgaatg acagcggttg atttcaattg
    64441 tatcattagc atattttcta agtaaagcct cttttaaatt gttacttttt ttatctatca
    64501 atgcgtttct gtaaaaattc caagaatttt ctttttcatc ccaatctttt ttgtgaaggt
    64561 caataatctc taaagttaga ttttcaatat tatttttatt attcattaga ttgttatgta
    64621 atggaatctt agcgatttct ccaacattaa tagcaatggt aggattgatg atattgatta
    64681 tattatagct tagtttggag tttagtaagc ataaaattgt tgggcgaagg ttatcttcac
    64741 aaaagccaga ggatcctcta ccatcaaaca gaaagccttg cggtaaaact ctaaaagcat
    64801 tactacctga cgtaacatca gaccaagtaa taccctttct aaatataaaa tctagattgt
    64861 aattatgtga tcgaacttta ccattttcaa ttccaaaatt tcttattttt tctccatcat
    64921 tctcccaatt aacaacgtaa tcatgattgc cgtaccactt tctatagcta ccacctttgg
    64981 cataagggaa ccattttttt aatgagttcg atgcttcttc tctatctttg caaccaaaac
    65041 caatattttg tatagaaacc tcatgccaga aacgcaagaa ctcatcattg ttagctgtag
    65101 acatcccaag acgagtatca aaatattctg aaattggttt tgaagtctcg aaaacattta
    65161 aaagagaatg actgatccag taagctatag gagaaccagg gattttctta aaatcatttg
    65221 tagtggccgt ttttaaacgc tcatttatta ccgggaattg ttttggattg ccttcttgat
    65281 ttatattacc gttttcgaca taagagaatg aaccttggta agtcgaaatg tggttgtttc
    65341 taaatacagt ggcattagtt ccgaaggcaa ttttcataac tccattgccc atatgcacca
    65401 tagtttcgat tgtataatta gtgaggatat tttcacgcat gttttcaaaa ctggaaagga
    65461 acatccagga ttgcattgtt accattgagt tgaaaccagc atttttcaac caaaggaaac
    65521 cacgctctat aaacatagca aacaaatcag acttactatc tgggaaatta tttttggcaa
    65581 actctttcag ctcaccattc attcccttac tgcccatata cggaggattc gccaccactg
    65641 catcatatcg ctgcgctaaa atccacgcct gctgaataaa cggaataaac gctttcgccg
    65701 ccgctttttg ctggaaatcg ccttcctgtt ccaggcgata cagcgcgtcc agaaatactt
    65761 tcagttccgc ttcttcttcc tgcggcacct gaatcagcga tcccagcgtt tttgcattca
    65821 caaagcgttt caacgtgcgc atcagcagct gatattctgc gctgtcggtt tgggctaacg
    65881 tgatattttc agcaaacata tcccccatac taccggtttg tacctgctgg tggaaattga
    65941 gctgctgcca gagcttggcg atatccagat gcaggctttc ctgtaaagag agaatattca
    66001 gacgtacatc gcgggtaaat attctgcggt catcctgacg tgccatcatt aataatgcaa
    66061 agccggaaag ctgtgccgct cggtcgtcga tatcaagacc aaaaatatta ttttccagaa
    66121 tcagttgtgg aatatcacgc ccgcgataac cacgctcttc gtagatattt ttcagtacat
    66181 tataggcttc aatcaaaata tgaccggagc cgcaggccgg gtcgagcact ttaatacttt
    66241 cgggttcaat gctggccggg gtaatcgccg ccagctgtgc ctgcacttcc ggcgtttgtt
    66301 ccgcaggctc gatgtagtac tccattttgt ctttcagcgg cgagtccggg taggtctgca
    66361 accactggcg gccaacggag ttctgtacca gatactgcac aatccagttc ggcgtaaaca
    66421 gctgggtggc ggcaggaata tcttcgctct tcaccacttt gccaatcaca gcatctttct
    66481 tttctgaaat atagaactga tacagccagc cgataacctc tacctgttcc cagtcttctt
    66541 ccggaatatc atcaaccagc ccacgcagaa tggagtcggt acgggtcagg ttatccggca
    66601 gcagcagttc cgcttcgtca tctaccgctt caaacaggaa cggcatcgcg tggtgcaggg
    66661 cgtggcactg ccccagcagc agttcgcggt acagagcttc atcctgatta ccggaaagct
    66721 tcatctcaac cagctgcgct ttactttccg gcagcagggc ttctgccact tccggcacat
    66781 gatccagcac ctcaaacgcg gtcggcgtct ccggatggga caacatacgg aagccgtgct
    66841 caagataacc gtgcagctcc atatagcgta tcgcacataa gcggttaaac caggtgtagg
    66901 cgcagtgctc aaccagcacc tcaaaaccct gctcacgggc gcgttttacc agccgttcgc
    66961 ggcggggaag ggtcgataac ggataatcaa actgaccgta acgcacggtc tcgccaatgg
    67021 tttcggcctc ggcaatctgc aaattgcctt ttttatctgc agcgatccca agcgtcgtta
    67081 gcttctgaat caccgcatcg cggaaatcgt tacgggcctg tggggcatat tttttaatgt
    67141 tattggtatt catagaaaat cctgcaaagg gcagcagagt gctgccctga aatgggagtt
    67201 actgaaggcg aatacgatcg cctgctttaa tggcggccag cagcgttgtg cgtaacgtgt
    67261 cgagcgcctt ttccacctgt tcggtcgttt ccagaacctc accaccggtg gcattacgca
    67321 tctcactggc gacattcacc agatgcgttt ttttcggtac cggttggata acgggttttt
    67381 ccaccacaac ataagtctgt tctgactcgg cagcgcgttt ggcttcattt gctgcccgga
    67441 gttgtgcctc ttgcttttta cgcagctcgt cgatccacag gttaatcttt ttatctgcat
    67501 ccatttgcag cgcttttgtc tcttgctgtt ccgccaaaat ttccggaatc gactggctga
    67561 cctcagcgcg ttttctggct ttttgcatcg gcagcagaac gctgttttgc agttctgacg
    67621 tggcgtgcgc ttccagcaag cgttgacggc tttcctcaat gcgggcgtca atgcgttcga
    67681 gggcgtgctg gcggtgctgt tcgactaact gatggttgac gttctggacc tgttcaatca
    67741 acggcgtgat gcgattgaga tgcttataag gttccggcat ttgccagatg ctttccagct
    67801 cgcccagcgc tttaaccgct gcggcgtctt tttccagcgc gctgcggtta gatttaaagc
    67861 tgccattcag cgcagccccc agtttttgcc aggtggcgaa ttgcttacgg tagaagtcga
    67921 ccaaatcttc acggtcttca ctgaaatcca gaagttcatc tttcagcgat gagacttttt
    67981 cgatcagggc aaaatcttct ttttcattaa gaatggcatt aagcaggcgc agaccggatt
    68041 caatctcatt tttccccgga ttgtttccgc cctctgcctt ggcgcggaat acgttcagct
    68101 cttgcttcca ctcttcaaat acctgacgaa tatgttcaac cagcgccggc tcttcccgtt
    68161 cgttaaacgg ctgctgagcg atgtcagcca tggtttgcgc ggctttacgc acctgcgctt
    68221 catcatgacg gcgaacttta tgcagacgca attcgctatg gcggcggctg ttattaaata
    68281 actcccacgc ctgttttcgc tcgacgttgt tgttttgctg gctgaagctg aatttacttt
    68341 tgcaggccag acgggctacc agcagtttca cttcgtcttc gggccagcca taagggcgac
    68401 gcgcgaaatg gttcagaata tcgcgtaaat acaccggttt attgtattcg atattcatgc
    68461 tgatccaggt ttctacctcg cgcatggcgt cggggttgga ctcttcgagg ttaccgagat
    68521 ccagttcggt gtcgttctca accgtcagta atgcatgaat ttcacgggag atgtcaccgt
    68581 taaaaggccg cagcatcttc agcttgccga aggtgttttc aatcacgtaa cggcaggctt
    68641 catcgacaat attcgatggc gtggaggatt ttttcggtaa gcgctcgcca atcgcccaga
    68701 catctgcttc tgcaagtagt gattccaact gaacacggag taatttttcc cggttgctgt
    68761 tttcacgcgc tttttctgag agcagggttg cctgttccgg acgttgcccg gcgttatctt
    68821 tgaggaactt ttcagtctgg acgactaaat caatatcgct ccaggtacgg ccctcttcgg
    68881 gcagacgaat caaaatacag ccgtcgcctt ctgacgtata agggcgacag gtcgcatcgc
    68941 tgttatagaa cgaataggtc ggatctttag gggtcaggat cttcaccacc agatcgttaa
    69001 gcactgcgcc gtctaatggg tgcccgttca ggaagcggct gatatcaaag tcttgcttgt
    69061 tagccggata acgatattta cggcttttca gaatgtcatc aaagatgatc gatgccagtt
    69121 ttttgttgat cgcagagaaa tcgacatcaa cgttacggat ctcgttttcg atctcttttt
    69181 cttcgttggt caggaacaca tatttatctt caacgcgcgc aatgagcatc aggcgttcaa
    69241 gcgtgttcag acttttttcg acccggcggc gcaactcaac tttatcggca tcgatcctat
    69301 cgatagacaa tgtgaccagg ttatccaggg tgcttttcag cgtttccaca taacggatca
    69361 ggaacagcgt tttcagcagg ttgccatcga actcatcaag aatgccattc tggcaagcct
    69421 gagtgatggt gcggctaacg gcaggctcca ggaagctctc aatggcggca tagaagcgcc
    69481 agaaaggcac cagagaatcc agcccttgcg ctgagatttg ctgcgccgca gtctggaatg
    69541 cctccagctg agaacgctca cccatggcca attgtttacc cgctgcacct ttcgtccgaa
    69601 tagattcaaa cactttttgc agaatctgat agtgccacgg gacaaacggg tagttgtcaa
    69661 cgaactcttc ttcgctggta aaaggacgta gtgcagtagt tgttgtaggg tcaaaagcca
    69721 gctggttacg caggatatcg cctttctctt gccacacttt tgccagcgcc gcttttgctt
    69781 cgtcagtttt taccaacaaa cgtttctgga taacttctga tgtgttagag ctggaaagtt
    69841 gcaggcgtgt tgaaaagcgc ccctggatct tggagaagtc ctgtccgtcg cgactgctca
    69901 taccaccgat tgccgcgtcg atatcagcct gcgaagtcac gataacccat gcgcggccac
    69961 cgcagattac cccaaggttt tcggtaatag tctgcagctt cagcatcatt tgcgtatttt
    70021 tgccaatgaa ctgaccgact tcatccacca taaagaggat gttcttaccg ttgtcatcca
    70081 gccactcttt tacccactgg caaaaattat tgatatccag cgggaagttt ttgtcgagtt
    70141 gttccaccca ttggcgggag gcttcaagac tctgctgtgt ggcctggctt aacgcttgtg
    70201 ccatgtcatc gctgataaag tagtaagcgt cgcgctcgtc ttcccagcgt gagccattga
    70261 tatcggcaaa cgcggcttta aaggtctcat actgaccgcg tttatctagc tcgcgctcaa
    70321 gatgggcaat atgcggaaaa tcagcgcagt agccaatgcg ttcgttgaaa actttcagga
    70381 agactttaag aatggcatct tctttgtcat ctacgttggc acgcgaatca atattgaaca
    70441 gaatgacttc agtcgggtaa tgcaccgctt tgttaatatc ggcaaggaat aatgcatctt
    70501 tgattttatc ttcaaagaag gagtaagcat tacgttccgt accgttatgt gtaactttgc
    70561 ggttagataa aagataagaa agaatcttaa taaagtgcga tttacctgaa ccgaagaagc
    70621 ctgaaaccca tacgccgatc ttgttttcca tacggatccg ttccgggcca gtggcaggaa
    70681 cataggattc gaagaaatgg cgaagatggt tttccagttc gcgggtgatg acatattcat
    70741 ctaactcgat gtacgcgctg gcatcatcgg tttgctctgc tttgactacc ccgtttatat
    70801 ttcgttttag aggtttttca aaaatctgtt caatattcat tgttatgctc ttcattgagg
    70861 attcaatgtt gctgcgggtc ccgtatctgg taccagtctg aaagcgcgat aataattttg
    70921 tgaaccggtg tctgtgagcg gggaaaggtt gtacccgctg taggtgccag gataaaacat
    70981 cagcagtggg gtgaacccca tgacatcctg caaggcactc atcagttcat gaccgcgtac
    71041 taatggccag gcgttgccca tgccggtaag gatgacaaaa tcctgggcag ccagatcgac
    71101 ttttttcgca ataaaatcag cgatcttttt ttgatttaac ggaccagcaa gctgcttttt
    71161 cagcgtctcg gtacccactt tgacttcctg ctggcagacg cgctcgaaaa ggccgcgttc
    71221 attgagcata ttgatgatga tttggaagac attcagacag gcaaatttat ggtccttttc
    71281 cagatgccgg agcagatatt tcaaatgctc ccgtacctgc agttcgcact gcgcgggata
    71341 atcaaagatc caaaaaccaa tttcatttcc ggagccgtta tttttgagga agcgatcttc
    71401 gttaatgcga ctctggattt gagacaggcg atattcaaga acgggatcta tcaccgtttt
    71461 ccctccagaa tagatatcaa gtcctgttgc cccaggcgct gtaacactgc ctgagtttcc
    71521 ggtaaaaggt aaactgcctg caggttacgt ctgcggggcg tatccacata acccgcttca
    71581 gcaagcgcct tcaccagatt gtttcccatt tttgcaatag atgaatctga gtagctggcg
    71641 agtaccggat gttggcgaac ctggttatta acaaattcgt tccaactatt gccaggcaac
    71701 ttttccttga actgcctgcg cagatcgttc accacttcag ccagaaaatc cttaactacc
    71761 ggcgaatgta gcgtcagagc cacaaacaga agttgctggc gttcccgctc gctaccttcg
    71821 gcaattaatg tccacgcgct tttatcaagc gtgttcaggc gaagcctgat tgttgccgcg
    71881 taacgttttg cggtattagg agaagaggct tgtaaaatgt tgtggccaac aatttgctct
    71941 tgccatgtct gttcatcggg atcggttagc aacagctcgg caatgacgcg gctttccctg
    72001 ctcatgagcg gcccgcccag caagtctcct atccatgcct tgtcattttt aatcatcgaa
    72061 aaaccctctg aagactggaa tgcagctcgc aaaaaacaaa aagtgattgt aatgggggac
    72121 tgggcgagat gccatgagaa aaggcaattt gtgagaggga tgatcgcgtt atgtacaaca
    72181 cataaacggt tttggcgaga gtcatgcttt agccatttct atgagtgaga ctggaaatta
    72241 ccctatatca ttcccattga atgcatcagc ttaatcgatt acagctcagg aaataaaatt
    72301 ttaactgtgt taaagtccct gtaaaattag ttaaaaaagg aagataaacg tggcgctgta
    72361 caatatcgca aataaagaac tacatgcttt agaaaaaacg acctttactc tggaaggatt
    72421 acaggaacgt tatgatttac aggaagcaat caaaaaaaac atagatatta ttgctcctga
    72481 ttgcctggta atttccgaag agttttctga ctgggaagat agtcgtcggc gaattgattt
    72541 attggcaatc gataaacagg caaatttagt ggttatcgaa ttaaaatgtg atgaaacggg
    72601 tgcgcatatg gagttacagg cactacgata tgcggcgatg atttccacaa tgagttttgc
    72661 gaaagcatgt gaatatttcc agacgtattt gaaaaaacaa aactgcgatg ccgatgcgaa
    72721 agaaaaaata cttgaatttg ttgagctgga tgaaactgag cttgttgatt tcggaaaaga
    72781 tatcagaatt gttttggctt catcggattt tagcaaggaa ctgactacca ctgcgatttg
    72841 gttaagagat aagggggtag acattcgatg cgttcgtctg acgccttata gatttaatga
    72901 tgatgttctt atcaatgcag agcaaatcat accggttcct gaattggaag aatatcaagt
    72961 taaatttcgg gaaaaacgtg atgaacagct gattagcagt cagaaaaaag agaaggacta
    73021 tacgtggtat atatataaag ataaagaact taataaacgt aaattagctc ttgaattatt
    73081 acgtgattgg ataagacaat ttaatcctgc tagttataat gatttaataa atggattaag
    73141 tgaagacttt aaaaaacgta cagttatgct tgtcgatcag ataccagaga agcaaaagag
    73201 tcgctatcat atcaatgaag atgcgttgat tacactccct tctggtgaaa ttgttgctat
    73261 ttctaatcaa tggggcattg ttaatattga actgttgatt gagtttgtac ggcaaaatgg
    73321 ttttgttgtt gaaaaagctg aacaataaat tttgatattg ctataaatgt ctacattgaa
    73381 aaatagggag aacatttgta gcttttaaca tctcaatgca cgagatggca agattatggg
    73441 ggcgcggtat tatcaacctc accccctaaa aacctctttc cgatgccact ctacaaaccc
    73501 ctcaaaaggg taatttttac gcacctgtgg taacgcaata gttttcccgt caaaatccca
    73561 gaatagtttc tcaacgatcc ctccgccgtt taccgcatct gataccagta cccgcatatt
    73621 ttcatccagg ccaatcgatc ctttatcaaa ggctttatgg tgaattgcgc aaagcgccag
    73681 gccattagga atttcacaag gacctccttg ctgtttccat tttatatgcg ccgcttccaa
    73741 cgcgacggtg gtgtcgtcgt ggcgcatatt aaaaccacat atcgcgcact ggtagttata
    73801 agcgcgtaat acatttttgc ggaataacgg gtcgcgctgt ttacggattt gctggagatc
    73861 aaaacccagc tcgtcagcaa tttcttcctg aatactctcg gtgaagtgcg cttcgagtat
    73921 ctgttgcgcc agagtattga tgagtttttt attgccggtt accagggcgt agtgctgttc
    73981 atcgaaaccg cctgcaacgt ggtattcgtt cagttccttc actggcggtt gtcggctgct
    74041 ccctgctgtt gagcaaagct cggcattgtg taattgccag aatccgtcgc cttgtaatcg
    74101 ccagaacggc atatcaggtc ggtactgtga acgctgtggc ccaaaacgtt ccagtaaact
    74161 gtgcagaggt tcgtagattt ccgatccata atcgaaaagg cgtggatgtc cattcaggta
    74221 tcccgctaat acgtatagca ataacaatgg tttatgcggt gcgcgctgtt cacccttatg
    74281 ccagattttt atattggcaa ttgcctgctg tagcgattta ctggaagcca ttgatatccc
    74341 tggcagtgaa ataattgcga tcatgcttgc aaattttctg ctgttcaagc ctgcaagtga
    74401 agatatatta cattgatctc gtttgttttt ttgctgcctg tgtaccctaa ttgccatgcg
    74461 atgctgcacc ggcgcaaacc accgtttacg ccggaggagt taaaagcgtt gatggatgcg
    74521 aataaatcaa attaatgaac gctcactttt cgcgccaaaa ctgcaaaatt gcttctacaa
    74581 acctgtcaga aataaccccc cgacgggctg ccgtgctgaa atgactgctt cgcagtgccg
    74641 tcgtccagtt ctccggtgac cggtacgctg aaccgtaata tcccgtgctt aaatcgtccg
    74701 ggtcttcatc aggcgcgttc gtcagtcctc tttcaccttg ttgctggaag tcgacgaaaa
    74761 taatattggc taaaacatta ttttgataat tgccataaat agcttcatac gaatgcggga
    74821 aattttcttt ccagtaccag gtggtatcgc cgcaaaacca cggcgcgtca gtaatattat
    74881 taagctgaga atggtattgt tttagatccc tacgaaaggc ttcaaccata tgattaaagt
    74941 gttgagggtg tgacgcgtag tcactggtca ttaagtcaaa ttcgccttgc atccagcata
    75001 cgccgaggaa tttgttctgc ggattttttg ccagtgcggc tcgcgttctg ctgactaaat
    75061 cctggtacag cggagtatcc gttccccaac gacaagcatc atggctggct ccgtgccgtt
    75121 ctgaatatgt cccttcgctg cccgcggtaa aagccgatcc gccacggcaa catggaacga
    75181 tgagaacccc tgcattatca ggaataaagg gtagtaattt ccgtgcaata tgcagtgcct
    75241 ggccaacggt gccgtactgt gtttgatgat tcgttgccag aggatggtga taaccctgca
    75301 tatcctgaac atcgtgtggg cagtgagtca gtggaataat gtcgttaaag tgacatgacg
    75361 ggcctccggg atgcgtatgc gcaaatctcg ctaattgttt aattctggga tgaggcgcat
    75421 cttccctgtc cggtaatggc agtccttcgc catacgccat ggcattagac tgaccagcaa
    75481 cggtaagaac atagtaataa tcgggcgata ttattgcgtt catataaatt tcgcgtaatt
    75541 gaaatgacaa attgatagca gggccccttt accggggccc tgaagcgtta attttgtact
    75601 gtgactttat tatccttcac agagatcaaa actgaatccg tcaccgcttt gccgcctgca
    75661 gtttcaccgc caataatcaa tagactatta ttccagggca atgatactcc gtaggcccga
    75721 ccttgcgata attcacccga tttatcccat ttcccgttat gccaaagatg aatatcagtg
    75781 ctatatgatt tttttaggcc ttcatgcgca tagttcttac cgttctggta attttctcgt
    75841 gaacctttga atccggcccc tccggcaaat ataagagaat cattgcttat ccccgcaaaa
    75901 ccgccagcga cgccatctgg tgatgagacg ggagcaagct tattccattt taaattatta
    75961 ccggtgaaat caagttcaaa tacggcatcc gttcgcaatc ctggtttggc ttcgccatta
    76021 ataagccagg ttttatcacc tttattcaca accgccgcac cagccgttcc gtaccaggga
    76081 gattcgccag cgtaactcca ttgctgtgtt gagggatcaa aagacaacag aaacttattg
    76141 aagaaataat cttctgcttt tttgtcaaaa taatgggcat tgatcttgtc tatagttgct
    76201 gaatcttttc cagc
//
LOCUS       DAFATA010000010        75550 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFATA010000010 DAFATA010000000
VERSION     DAFATA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.010"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..539)
                     /locus_tag="LGJ20_000991"
     CDS             complement(<1..539)
                     /locus_tag="LGJ20_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HBK2385100.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGI"
     gene            complement(561..1508)
                     /gene="cyoA"
                     /locus_tag="LGJ20_000992"
     CDS             complement(561..1508)
                     /gene="cyoA"
                     /locus_tag="LGJ20_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBK2385101.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(1968..3443)
                     /gene="ampG"
                     /locus_tag="LGJ20_000993"
     CDS             complement(1968..3443)
                     /gene="ampG"
                     /locus_tag="LGJ20_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBK2385102.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDT
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(3487..4065)
                     /locus_tag="LGJ20_000994"
     CDS             complement(3487..4065)
                     /locus_tag="LGJ20_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2385103.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            4370..4687
                     /gene="bolA"
                     /locus_tag="LGJ20_000995"
     CDS             4370..4687
                     /gene="bolA"
                     /locus_tag="LGJ20_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBK2385104.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            5031..6329
                     /gene="tig"
                     /locus_tag="LGJ20_000996"
     CDS             5031..6329
                     /gene="tig"
                     /locus_tag="LGJ20_000996"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK2385105.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            6575..7198
                     /gene="clpP"
                     /locus_tag="LGJ20_000997"
     CDS             6575..7198
                     /gene="clpP"
                     /locus_tag="LGJ20_000997"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK2385106.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            7324..8598
                     /gene="clpX"
                     /locus_tag="LGJ20_000998"
     CDS             7324..8598
                     /gene="clpX"
                     /locus_tag="LGJ20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBK2385107.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            8786..11140
                     /gene="lon"
                     /locus_tag="LGJ20_000999"
     CDS             8786..11140
                     /gene="lon"
                     /locus_tag="LGJ20_000999"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBK2385108.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            11349..11621
                     /gene="hupB"
                     /locus_tag="LGJ20_001000"
     CDS             11349..11621
                     /gene="hupB"
                     /locus_tag="LGJ20_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBK2385109.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            11813..13684
                     /gene="ppiD"
                     /locus_tag="LGJ20_001001"
     CDS             11813..13684
                     /gene="ppiD"
                     /locus_tag="LGJ20_001001"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK2385110.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEVVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            13835..14206
                     /locus_tag="LGJ20_001002"
     CDS             13835..14206
                     /locus_tag="LGJ20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK2385111.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            14300..14698
                     /gene="fadM"
                     /locus_tag="LGJ20_001003"
     CDS             14300..14698
                     /gene="fadM"
                     /locus_tag="LGJ20_001003"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBK2385112.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(14750..15445)
                     /gene="queC"
                     /locus_tag="LGJ20_001004"
     CDS             complement(14750..15445)
                     /gene="queC"
                     /locus_tag="LGJ20_001004"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBK2385113.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(15510..17210)
                     /locus_tag="LGJ20_001005"
     CDS             complement(15510..17210)
                     /locus_tag="LGJ20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBK2385114.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            17310..18128
                     /gene="cof"
                     /locus_tag="LGJ20_001006"
     CDS             17310..18128
                     /gene="cof"
                     /locus_tag="LGJ20_001006"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBK2385115.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            18281..18739
                     /gene="decR"
                     /locus_tag="LGJ20_001007"
     CDS             18281..18739
                     /gene="decR"
                     /locus_tag="LGJ20_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBK2385116.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            18769..20541
                     /locus_tag="LGJ20_001008"
     CDS             18769..20541
                     /locus_tag="LGJ20_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBK2385117.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            20534..22315
                     /locus_tag="LGJ20_001009"
     CDS             20534..22315
                     /locus_tag="LGJ20_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBK2385118.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            22496..22834
                     /gene="glnK"
                     /locus_tag="LGJ20_001010"
     CDS             22496..22834
                     /gene="glnK"
                     /locus_tag="LGJ20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK2385119.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            22864..24150
                     /gene="amtB"
                     /locus_tag="LGJ20_001011"
     CDS             22864..24150
                     /gene="amtB"
                     /locus_tag="LGJ20_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBK2385120.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(24198..25058)
                     /gene="tesB"
                     /locus_tag="LGJ20_001012"
     CDS             complement(24198..25058)
                     /gene="tesB"
                     /locus_tag="LGJ20_001012"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBK2385121.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            25276..25848
                     /locus_tag="LGJ20_001013"
     CDS             25276..25848
                     /locus_tag="LGJ20_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBK2385122.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(25879..26190)
                     /locus_tag="LGJ20_001014"
     CDS             complement(25879..26190)
                     /locus_tag="LGJ20_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBK2385123.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            26353..26449
                     /gene="ffs"
                     /locus_tag="LGJ20_001015"
     ncRNA           26353..26449
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LGJ20_001015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            26569..26922
                     /locus_tag="LGJ20_001016"
     CDS             26569..26922
                     /locus_tag="LGJ20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBK2385124.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(26964..28514)
                     /gene="pdeB"
                     /locus_tag="LGJ20_001017"
     CDS             complement(26964..28514)
                     /gene="pdeB"
                     /locus_tag="LGJ20_001017"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBK2385125.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEKLDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(28678..29148)
                     /locus_tag="LGJ20_001018"
     CDS             complement(28678..29148)
                     /locus_tag="LGJ20_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBK2385126.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(29264..29815)
                     /gene="maa"
                     /locus_tag="LGJ20_001019"
     CDS             complement(29264..29815)
                     /gene="maa"
                     /locus_tag="LGJ20_001019"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBK2385127.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(29987..30205)
                     /gene="hha"
                     /locus_tag="LGJ20_001020"
     CDS             complement(29987..30205)
                     /gene="hha"
                     /locus_tag="LGJ20_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBK2385128.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(30231..30605)
                     /gene="tomB"
                     /locus_tag="LGJ20_001021"
     CDS             complement(30231..30605)
                     /gene="tomB"
                     /locus_tag="LGJ20_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBK2385129.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(31151..34300)
                     /locus_tag="LGJ20_001022"
     CDS             complement(31151..34300)
                     /locus_tag="LGJ20_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBK2385130.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(34323..35516)
                     /gene="acrA"
                     /locus_tag="LGJ20_001023"
     CDS             complement(34323..35516)
                     /gene="acrA"
                     /locus_tag="LGJ20_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBK2385131.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            35658..36305
                     /gene="acrR"
                     /locus_tag="LGJ20_001024"
     CDS             35658..36305
                     /gene="acrR"
                     /locus_tag="LGJ20_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBK2385132.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            36433..39795
                     /gene="mscK"
                     /locus_tag="LGJ20_001025"
     CDS             36433..39795
                     /gene="mscK"
                     /locus_tag="LGJ20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBK2385133.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETDLRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(40007..40168)
                     /gene="rsmS"
                     /locus_tag="LGJ20_001026"
     CDS             complement(40007..40168)
                     /gene="rsmS"
                     /locus_tag="LGJ20_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBK2385134.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(40182..40709)
                     /gene="priC"
                     /locus_tag="LGJ20_001027"
     CDS             complement(40182..40709)
                     /gene="priC"
                     /locus_tag="LGJ20_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBK2385135.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            40779..41156
                     /locus_tag="LGJ20_001028"
     CDS             40779..41156
                     /locus_tag="LGJ20_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBK2385136.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            41309..41860
                     /gene="apt"
                     /locus_tag="LGJ20_001029"
     CDS             41309..41860
                     /gene="apt"
                     /locus_tag="LGJ20_001029"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK2385137.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            41989..43920
                     /gene="dnaX"
                     /locus_tag="LGJ20_001030"
     CDS             41989..43920
                     /gene="dnaX"
                     /locus_tag="LGJ20_001030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK2385138.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    43253..43317
                     /gene="dnaX"
                     /locus_tag="LGJ20_001030"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            43973..44302
                     /locus_tag="LGJ20_001031"
     CDS             43973..44302
                     /locus_tag="LGJ20_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK2385139.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            44302..44907
                     /gene="recR"
                     /locus_tag="LGJ20_001032"
     CDS             44302..44907
                     /gene="recR"
                     /locus_tag="LGJ20_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK2385140.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            45017..46891
                     /gene="htpG"
                     /locus_tag="LGJ20_001033"
     CDS             45017..46891
                     /gene="htpG"
                     /locus_tag="LGJ20_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBK2385141.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            47072..47716
                     /gene="adk"
                     /locus_tag="LGJ20_001034"
     CDS             47072..47716
                     /gene="adk"
                     /locus_tag="LGJ20_001034"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK2385142.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            47952..48914
                     /gene="hemH"
                     /locus_tag="LGJ20_001035"
     CDS             47952..48914
                     /gene="hemH"
                     /locus_tag="LGJ20_001035"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBK2385143.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(48911..49870)
                     /gene="aes"
                     /locus_tag="LGJ20_001036"
     CDS             complement(48911..49870)
                     /gene="aes"
                     /locus_tag="LGJ20_001036"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBK2385144.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            50022..51326
                     /gene="gsk"
                     /locus_tag="LGJ20_001037"
     CDS             50022..51326
                     /gene="gsk"
                     /locus_tag="LGJ20_001037"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBK2385145.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(51409..53085)
                     /locus_tag="LGJ20_001038"
     CDS             complement(51409..53085)
                     /locus_tag="LGJ20_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBK2385146.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(53323..54543)
                     /gene="fsr"
                     /locus_tag="LGJ20_001039"
     CDS             complement(53323..54543)
                     /gene="fsr"
                     /locus_tag="LGJ20_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBK2385147.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            54761..56413
                     /gene="ushA"
                     /locus_tag="LGJ20_001040"
     CDS             54761..56413
                     /gene="ushA"
                     /locus_tag="LGJ20_001040"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBK2385148.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(56450..56929)
                     /gene="ybaK"
                     /locus_tag="LGJ20_001041"
     CDS             complement(56450..56929)
                     /gene="ybaK"
                     /locus_tag="LGJ20_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBK2385149.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(57133..57927)
                     /locus_tag="LGJ20_001042"
     CDS             complement(57133..57927)
                     /locus_tag="LGJ20_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBK2385150.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            58065..58406
                     /locus_tag="LGJ20_001043"
     CDS             58065..58406
                     /locus_tag="LGJ20_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBK2385151.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(58520..61024)
                     /gene="copA"
                     /locus_tag="LGJ20_001044"
     CDS             complement(58520..61024)
                     /gene="copA"
                     /locus_tag="LGJ20_001044"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBK2385152.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRSSTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            61286..62218
                     /gene="glsA"
                     /locus_tag="LGJ20_001045"
     CDS             61286..62218
                     /gene="glsA"
                     /locus_tag="LGJ20_001045"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK2385153.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGEWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            62221..63513
                     /locus_tag="LGJ20_001046"
     CDS             62221..63513
                     /locus_tag="LGJ20_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2385154.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            63638..64045
                     /gene="cueR"
                     /locus_tag="LGJ20_001047"
     CDS             63638..64045
                     /gene="cueR"
                     /locus_tag="LGJ20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBK2385155.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(64046..64504)
                     /locus_tag="LGJ20_001048"
     CDS             complement(64046..64504)
                     /locus_tag="LGJ20_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK2385156.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(64501..65418)
                     /locus_tag="LGJ20_001049"
     CDS             complement(64501..65418)
                     /locus_tag="LGJ20_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBK2385157.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            65564..66241
                     /gene="fetA"
                     /locus_tag="LGJ20_001050"
     CDS             65564..66241
                     /gene="fetA"
                     /locus_tag="LGJ20_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBK2385158.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            66228..67007
                     /gene="fetB"
                     /locus_tag="LGJ20_001051"
     CDS             66228..67007
                     /gene="fetB"
                     /locus_tag="LGJ20_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBK2385159.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(67070..67924)
                     /gene="cnoX"
                     /locus_tag="LGJ20_001052"
     CDS             complement(67070..67924)
                     /gene="cnoX"
                     /locus_tag="LGJ20_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBK2385160.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(67985..68794)
                     /gene="ybbO"
                     /locus_tag="LGJ20_001053"
     CDS             complement(67985..68794)
                     /gene="ybbO"
                     /locus_tag="LGJ20_001053"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBK2385161.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTIRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(68784..69407)
                     /gene="tesA"
                     /locus_tag="LGJ20_001054"
     CDS             complement(68784..69407)
                     /gene="tesA"
                     /locus_tag="LGJ20_001054"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBK2385162.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            69378..70064
                     /locus_tag="LGJ20_001055"
     CDS             69378..70064
                     /locus_tag="LGJ20_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2385163.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPTQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            70061..72475
                     /locus_tag="LGJ20_001056"
     CDS             70061..72475
                     /locus_tag="LGJ20_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2385164.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGTIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(73188..74282)
                     /gene="mnmH"
                     /locus_tag="LGJ20_001057"
     CDS             complement(73188..74282)
                     /gene="mnmH"
                     /locus_tag="LGJ20_001057"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBK2385165.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(74351..75277)
                     /gene="allS"
                     /locus_tag="LGJ20_001058"
     CDS             complement(74351..75277)
                     /gene="allS"
                     /locus_tag="LGJ20_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBK2385166.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
BASE COUNT        18463 a        19178 c        20099 g        17810 t
ORIGIN      
        1 tctattcccg atagcggtgg ataggccagc cagccggtct gcgcaaattc gcccacgccg
       61 agagaaacgt taaccagaat cacaccaaca acagtaaacc agaagcttaa gttgttgagg
      121 aacgggaacg caacgtcacg cgcgccgatc tgcagcggaa ccaccaggtt catcagaccg
      181 ataacgaaag gcatcgccac gaagaagatc ataatcacgc cgtgcgcggt gaagatctga
      241 tcgtagtggt gaggtggcag gaagcccgct tcacccgccg aggcaagagc ctgctggcta
      301 cgcatcataa tggcgtcagc aaaaccacgc agcaacatca caatcgccac gatgatatac
      361 atgataccga ggcgtttatg gtcgacggag gtcagccact ctttccacag gtaggtccac
      421 ttaccgaagt aagtgatcag gccaacgagc gccagacctc ccaaaataat gccagcgatc
      481 gtaaccatga cgataggttc atggaacggg actgcatcaa gtgataattt tccgaacatc
      541 tttattcttc ctcaacccct ttaatgggcg gattccgcgt ggctcatgtc catgccttcc
      601 ataccttcgt gtgcactgtg ctcaccttct ggctgggtca tgtccatgct cttaccgtga
      661 gccataaact tgttaatcac atcggcaaac aagtctggtt tcacgttgga gaaatattcc
      721 acctggttgt attcgctagg cgcggccagt ttttcgaacg cagccatgtc agacatggtg
      781 ttcggcgact gtttcgcttt tgcgacccat tggtcgaatt cggcgcgatc cggtgttgca
      841 atagctttga acttcatgcc tgagaagccc ggcccgctgt agctggcgga gataccgtca
      901 taagtaccgg gttcgttggc gatcagatgc aggcgagtct gcataccggc catggcataa
      961 atctggctac ccagacgcgg aatgaagaag gagttcatca cggagttgga ggtcactttg
     1021 aagtacaccg gagtgttcgc cgggaaagcg atttcattca cggtagcaat gccctgttcc
     1081 gggtagatga agaaccattt ccagtccatg gaaaccactt cgatggtaat gggcttctcg
     1141 tcgtgtgcca gcggcttgct aggctcaaga gcgtgagtgg ttttccaggt cagtaccgca
     1201 aggaagatga tgattaagat aggcaccgtc cagaccacag cttccacttt attggagtgt
     1261 gaccagttcg ggctgtactt agcatcttta ttgctcgcac ggtacttcca ggcgaaacca
     1321 acagccatca agattgcggg aataacgaca atcaacatca ggccaaatgc cgtcagtatc
     1381 agtgaacgtt gctccagacc aatctgtcct ttgggatcta acagcgcaga attacagcca
     1441 ctgagcaata cagtgcctgc aaataatgac aaccatccca aacttttatt gtatttcctg
     1501 agtctcattt aacgacctca attccacggg atctggtggc gtttaaagtg tgtgggcatt
     1561 ttacgggaag gttacattac tgtaaacatg attaaattcc tgttacttgg atttggctgt
     1621 caggtcacat atgttgcaaa acatatcaag ttgattttaa gaagctgttg caaaagggcg
     1681 ttataacgaa aggggagtgc agaaggagtt tggacgggca attggtataa ccaatacaaa
     1741 ataaaacaat tatttaacaa ataattatca ttaaggtgac aactgggtag tgagaaaata
     1801 aaaactaatt agctgaatcg tgtaagaaaa atagaattca taaatagcgt caataatttc
     1861 caaacgctaa gccgcacaaa agaacaaata ttattaagcg atactgatgt ttatttagcc
     1921 gtaataatta cggcgagtga ttactacagc taaataatat ttacagatta cgtcagatgc
     1981 gtttttcgta gagccagata atccagcaaa ccaccaagca cgacgccaga aagggcgact
     2041 aaaaccccga cttccagcag agcaggcagg aaagagaagt gcgtcaaatc cagcgtgtcc
     2101 atcgtcaaca gcagtaacca cacggccaac aggctgacgc ccgccgccag tgtccacatg
     2161 gcaaaggcat aacctgccgg atattcggta cgggagataa agttgtcatt tactcgtgta
     2221 tattcaagcg tctggcggca aaccagcagc aaaataagcc ccggtacggc agcggcgacg
     2281 gagaatagat agaatgtcga ccagccgtgt gcttcaacaa accaacccgc cacggggccg
     2341 acataaactc gtcctacagc ggaaagcgct gagagcaggg caaattgagt agcggaaaat
     2401 gacttattac acagcgtcat taacagcgcg acaaaggctg atgtgcccat cccgccacag
     2461 aggttttcga aaaagacggc tgcgcccatg ctgtagagat gcttatcagt aatcgacagc
     2521 agccagtaac cagcgttaga cgcaccttgt aaaatgccga aaatcagcag cgcccggaac
     2581 agcgacaggc gctgcatcaa aatcccaccg tacaatgcgc caacaatggt cgccagtaag
     2641 ccaagcgttt tgttaaccac gcctacttca cccgcatcaa acccgacgcc gcgaatcaaa
     2701 aacgtggttg tcaggctcat ggcgaatgcg tcgcccagct tatacagcac gataagaagc
     2761 aaaataagcc aggcattatt gcgaccaaag aaatctcgca gaggtgcaac aaccgcttgt
     2821 tccagcgttt ttggcacagg aatggtgtcg gtcggttctg gtgcaagcaa cgtcgcgata
     2881 atacagggga tcaacagcgc cgccatcaac cagtacatgc cctgccagcc cagccattta
     2941 tctgccagcc acagggctaa gccgccggaa accagcatcc ctaaacggta acccagaacg
     3001 ctgattgccg cgcccgcacc acgttcttct gccgggagca catcggtttt ccacgcatcg
     3061 aagacaatat cctgggaggc cgagcaaaaa gcgatcacca ccgccagtgc cgccatccag
     3121 cggagttggg tgccaggttc gagaaaaccc atcgccgcaa tggcgactaa taacaggatt
     3181 tgcgtggcga gcagccaacc gcgccgccgc ccaaaaaatg ggggcgtgta gcggtccatc
     3241 agcggtgacc agaggaattt aaaaacgtaa gcctggccta ccagagagaa gaaaccaatg
     3301 gttttgagat caatattctc gaccgtcatc caggcctgta aggtgccgga tgtcagggcg
     3361 agcggtagcc cggaagcaaa acccaggatc agcaatatgg ctgaacgcgg ctgttgaaaa
     3421 atacgtaaat attgactgga catgggcgtc tacaggcccg gtttgcaccg ggccagaggg
     3481 cagaaattaa cgcgcgttct gcttgataaa ttcgtgaatg ctggtgtcct gagacatatc
     3541 agcgatggta tcggtcagca cactgttaac cgcatcggcg atatttttgt tggaggcctg
     3601 gaacgcacct tcaacgttgt agctggcacg atagtttttg gtcattttgt tgccattctg
     3661 cgcggtagcg atgatggcga tatccgcttt ggtagcgatg ttatagcgca cgttgccctg
     3721 ggacacgtca gcatacagtt ggctaacgat gatttgcaga ttaaccgggc cattcggacc
     3781 aaccatgtaa ccacgcgcgg tcatctgttt ttccagcact tcttgcagca ggaaacgcag
     3841 atctcgggag gcggtcaggg taacgatttg attatcgcgg gtgacttttg ccagcgcctg
     3901 atcggtacgc tgatcggcac cattaatgct tacggtgacg cccatcaggc ttggatcctg
     3961 ctgtggcagt gtaatcgtcg gggaaacttc aatagttgtt ggcggttttg cgcatcctgc
     4021 aagcataaac agagcaacta acgggaagag gatttttttg aacatgtttg ggctctcaga
     4081 gactcttaag cgtgtttggt aaaaattccc gccatcataa cattgccaac ggcgagggga
     4141 agtgggtaag gcatgtaaat tcatcatgtt gacgaaataa tcacccctgg taaaagaaac
     4201 gctgatgcga ggcctgtgtt tcaatcttta aatcagtaaa cttcatacgc ttgacggaaa
     4261 aaccaggacg aaacctaaat atttgttgtt aagctgcaat ggaaacggta aaagcggcta
     4321 gtatttaaag ggatggatga catctcagcg ttgtcggagg agatatttca tgatgatacg
     4381 tgagcggata gaagaaaaat taagggcggc gttccaaccc gtattcctcg aagtagtgga
     4441 tgaaagctat cgtcacaatg tcccagccgg ctctgaaagc cattttaaag ttgtgctggt
     4501 cagcgatcgt tttacgggtg aacgttttct gaatcgtcat cgaatgattt acagtacttt
     4561 agcggaggaa ctctctacta ccgttcatgc gctggctctg catacttaca ctattaagga
     4621 gtgggaaggg ttgcaggaca ccgtctttgc ctctcctccc tgtcgtggag caggaagcat
     4681 cgcgtaaaaa cgcatttgca actgtcggcg cttttccagt atgttgctaa agattttatg
     4741 aaaaacggcc tgcgggccgt tttgttttgt ctggattttg cgctttttgc ccagcattca
     4801 gacgaaaatt gcccgggaat tgtgaaaaaa tacgcgacag cgcgcaataa ccgttctcga
     4861 ctcataaaag tgatgccgct ataatgccgc gtcctatttg aatgctttcg ggatgattct
     4921 ggtaacaggg aatgtgattg attataagaa catcccggtt ccgcgaagcc aacaacctgt
     4981 gcttgcgggg taagagttga ccgagcactg tgattttttg aggtaacaag atgcaagttt
     5041 cagttgaaac cactcaaggc cttggccgcc gtgtaacgat tactatcgct gctgacagca
     5101 tcgagaccgc tgttaaaagc gagctggtca acgttgcgaa aaaagtacgt attgacggct
     5161 tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg ttatggcgcg tctgtacgcc
     5221 aggacgttct gggtgacctg atgagccgta acttcattga cgccatcatt aaagaaaaaa
     5281 tcaatccggc tggcgcaccg acttatgttc cgggcgaata caagctgggt gaagacttca
     5341 cctactctgt agagtttgaa gtttatccgg aagttgaact gcagggtctg gaagcgatcg
     5401 aagttgaaaa accgatcgtt gaagtgaccg atgctgacgt tgacggcatg ctggatactc
     5461 tgcgtaaaca gcaggcaacc tggaaagaaa aagacggcgc tgttgaagca gaagaccgcg
     5521 taaccatcga cttcaccggt tctgtagacg gcgaagagtt cgaaggcggt aaagcgtctg
     5581 atttcgtact ggcgatgggc cagggtcgta tgatcccggg ctttgaagac ggtatcaaag
     5641 gccacaaagc tggcgaagag ttcaccatcg acgtgacctt cccggaagaa taccacgcag
     5701 aaaacctgaa aggtaaagca gcgaaattcg ctatcaacct gaagaaagtt gaagagcgtg
     5761 aactgccgga actgactgca gaattcatca aacgtttcgg cgttgaagat ggttccgtag
     5821 aaggtctgcg cgctgaagtg cgtaaaaaca tggagcgcga gctgaagagc gccatccgta
     5881 accgcgttaa gtctcaggcg atcgaaggtc tggtaaaagc taacgacatc gacgtaccgg
     5941 ctgcgctgat cgacagcgaa atcgacgttc tgcgtcgcca ggctgcacag cgtttcggtg
     6001 gcaacgaaaa acaagctctg gaactgccgc gcgaactgtt cgaagaacag gctaaacgtc
     6061 gcgtagttgt tggcctgctg ctgggcgaag ttatccgcac caacgagctg aaagctgacg
     6121 aagagcgcgt gaaaggcctg atcgaagaga tggcttctgc gtacgaagat ccgaaagaag
     6181 ttatcgagtt ctacagcaaa aacaaagaac tgatggacaa catgcgcaat gttgctctgg
     6241 aagaacaggc tgttgaagct gtactggcga aagcgaaagt gactgaaaaa gaaaccactt
     6301 tcaacgagct gatgaaccag caggcgtaat ttacgcagca taacgcgcta aattcgcaca
     6361 aaggcccgtc accgccaggt ggtgggcttt tttttgtcat gaattttgca tggaaccgtg
     6421 cgaaaagcct ctttcggtgt tagcgtaaca acaaaagatt gttatgcttg aaatatggtg
     6481 atgccgtacc cataacacag ggactagctg ataatccgtc cataaggtta caatcggtac
     6541 agcaggtttt ttcaatttta tccaggagac ggaaatgtca tacagcggcg aacgagataa
     6601 ctttgcaccc catatggcgc tggtgccgat ggtcattgaa cagacctcac gtggtgagcg
     6661 ctcttttgat atctattctc gtctacttaa ggaacgcgtc atttttctga ctggccaggt
     6721 tgaagaccac atggctaacc tgattgtggc gcagatgctg ttcctggaag cagaaaaccc
     6781 agaaaaagat atctatctgt acattaactc cccaggcggg gtgatcactg ccgggatgtc
     6841 tatctatgac accatgcagt ttatcaagcc tgatgtcagc accatctgta tgggccaggc
     6901 ggcctcgatg ggcgctttct tgctgaccgc aggggcaaaa ggtaaacgtt tttgcctgcc
     6961 gaattcgcgc gtgatgattc accaaccgtt gggcggctac cagggccagg cgaccgatat
     7021 cgaaattcat gcccgtgaaa ttctgaaagt taaagggcgc atgaatgaac ttatggcgct
     7081 tcatacgggt caatcattag aacagattga acgtgatacc gagcgcgatc gcttcctttc
     7141 cgcccctgaa gcggtggaat acggtctggt cgattcgatt ctgacccatc gtaattgatg
     7201 ccagaggcgc aactgtgccg ctatacttat ccagggcggc acaacgctgt aagcggcttg
     7261 cgcctgagaa tggcatttgc gtcgtcgtgt gcggcacaaa gaacaaagaa gaggttttga
     7321 cccatgacag ataaacgcaa agatggctca ggcaaattgc tgtattgctc tttttgcggc
     7381 aaaagccagc atgaagtgcg caagctgatt gccggtccat ccgtgtatat ctgcgacgaa
     7441 tgtgttgatt tatgtaacga catcattcgc gaagagatta aagaagttgc accgcatcgt
     7501 gaacgcagtg cgctaccgac gccgcatgaa attcgcaacc acctggacga ttacgttatc
     7561 ggccaggaac aggcgaaaaa agtgctggcg gtcgcggtat acaaccatta caaacgtctg
     7621 cgcaacggcg ataccagcaa tggtgtcgag ttgggcaaaa gtaacattct gctgatcggt
     7681 ccgaccggtt ccggtaaaac gctgctggct gaaacgctgg cgcgcctgct ggacgtcccg
     7741 ttcaccatgg ccgacgcaac cacgctgacc gaagccggtt atgtgggcga agacgttgaa
     7801 aacatcattc agaaactgtt gcagaaatgc gactacgatg tccagaaagc acagcgtggt
     7861 attgtctaca tcgatgaaat cgacaagatt tctcgtaagt cggacaaccc gtctattacc
     7921 cgtgacgttt ccggtgaagg cgtacagcag gcactgttga aactgatcga aggtactgtt
     7981 gctgccgttc caccgcaggg cgggcgtaag catccgcagc aggaattttt gcaggttgat
     8041 acctccaaga tcctgtttat ttgtggcggt gcgtttgccg gtctggataa agtgatttcc
     8101 caccgtgtag aaaccggctc cggcattggt tttggcgcga cggtaaaagc gaagtccgac
     8161 aaagcaagcg aaggcgagct gctggcgcag gttgaaccgg aagatctgat caagtttggt
     8221 cttatccctg agttcattgg tcgtctgccg gttgtcgcaa cgttgaatga actgagcgaa
     8281 gaagctctga ttcagatcct caaagagccg aaaaacgccc tgaccaagca gtatcaggcg
     8341 ctgtttaatc tggaaggcgt ggatctggaa ttccgtgacg aggcgctgga tgctatcgct
     8401 aagaaagcga tggcgcgtaa aaccggtgcc cgtggcctgc gttccatcgt agaagccgca
     8461 ctgctcgata ccatgtacga tctgccgtcc atggaagacg tcgaaaaagt ggttatcgac
     8521 gagtcggtaa ttgatggtca aagcaaaccg ttgctgattt atggtaagcc ggaagcgcaa
     8581 caggcatctg gtgaataatt aaccattccc atacaattag ttaaccaaaa aggggggatt
     8641 ttatctcccc tttaattttt cctctattct cggcgttgaa tgtgggggaa acatccccat
     8701 atactgacgt acatgttaat agatggcgtg aagcacagtc gtgtcatctg attacctggc
     8761 ggaaattaaa ctaagagaga gctctatgaa tcctgagcgt tctgaacgca ttgaaatccc
     8821 cgtattgccg ctgcgcgatg tggtggttta tccgcacatg gtcatcccct tatttgtcgg
     8881 gcgggaaaaa tctatccgtt gtctggaagc ggcgatggac catgataaaa aaattatgct
     8941 ggtcgcgcag aaagaagctt caacggatga gccgggtgta aacgatcttt tcaccgtcgg
     9001 gaccgtggcc tctatattgc agatgctgaa actgcctgac ggcaccgtca aagtgctggt
     9061 cgaggggtta cagcgcgcgc gtatttctgc gctctctgac aatggcgaac acttttctgc
     9121 gaaggcggag tatctggagt cgccgaccat tgatgagcgg gaacaggaag tgctggtgcg
     9181 tactgcaatc agccagttcg aaggctacat caagctgaac aaaaaaatcc caccagaagt
     9241 gctgacgtcg ctgaatagca tcgacgatcc ggcgcgtctg gcggatacca ttgctgcaca
     9301 tatgccgctg aaactggctg acaaacagtc cgttctggag atgtccgacg ttaacgaacg
     9361 tctggaatat ctgatggcaa tgatggaatc ggaaatcgat ctgctgcagg ttgagaaacg
     9421 cattcgcaac cgcgttaaaa agcagatgga gaaatcccag cgtgagtact atctgaacga
     9481 gcaaatgaaa gctattcaga aagaactcgg tgaaatggac gacgcgccgg acgaaaacga
     9541 agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa gaggcaaaag agaaagcgga
     9601 agcagagttg cagaagctga aaatgatgtc tccgatgtcg gcagaagcga ccgtagtgcg
     9661 tggttatatc gactggatgg tacaggtacc gtggaatgcg cgcagcaagg tcaaaaaaga
     9721 cctgcgtcag gcgcaggaaa tccttgatac cgaccattat ggtctggagc gcgtgaaaga
     9781 tcgcatcctt gagtaccttg cggttcaaag ccgtgtcaac aaaatcaagg gaccgatcct
     9841 ctgcctggta gggccgccgg gggtaggtaa aacctctctt ggtcagtcca ttgccaaagc
     9901 caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg cgtgatgaag cggaaatccg
     9961 tggtcaccgc cgtacttaca tcggttctat gccgggtaaa ctgatccaga aaatggcgaa
    10021 agtgggcgtg aaaaacccgc tgttcctgct tgatgagatc gacaaaatgt cttctgacat
    10081 gcgaggcgat ccggcctctg cactgcttga agtgctggat ccagagcaga acgtagcgtt
    10141 cagcgaccac tacctggaag tggattacga tctcagcgac gtgatgtttg tcgcgacgtc
    10201 gaactccatg aacattccgg caccgctgct ggatcgtatg gaagtgattc gcctctccgg
    10261 ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac ctgctgccga agcagattga
    10321 acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat agcgccatta tcggcattat
    10381 tcgttactac acccgtgagg cgggcgtgcg tggtctggag cgtgaaatct ccaaactgtg
    10441 ccgcaaagcg gttaagcagt tactgctcga taagtcatta aaacatatcg aaattaacgg
    10501 cgataacctg catgactacc tcggtgttca gcgtttcgac tatggtcgcg ctgataacga
    10561 aaaccgtgtc ggtcaggtaa ccggtctggc gtggacggaa gtgggcggtg acttgctgac
    10621 cattgaaacc gcatgtgttc cgggtaaagg caaactgacc tataccggtt cgctcggcga
    10681 agtgatgcag gagtctattc aggcggcgtt aacggtggtt cgtgcgcgtg cggaaaaact
    10741 ggggatcaac cctgattttt acgaaaaacg tgacatccac gtccacgttc cggaaggtgc
    10801 gacgccgaaa gatggtccga gtgccggtat tgctatgtgc accgcgctgg tttcttgcct
    10861 gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt gagatcactc tgcgtggtca
    10921 ggtactgccg atcggtggtt tgaaagaaaa actcctggca gcgcatcgcg gcgggattaa
    10981 aacagtgcta attccgttcg aaaataaacg cgatctggaa gagattcctg acaacgtaat
    11041 tgccgatctg gacattcatc ctgtgaagcg cattgaggaa gttctgactc tggcgctaca
    11101 aaatgaaccg tctggtatgc aggttgtgac tgcaaaatag tgacctcgcg caaaatgcac
    11161 taataaaaac agggctggca ggctaattcg ggcttgccag cctttttttg tctcgctaag
    11221 ttagatggcg gatcgggctt gcccttatta aggggtgttg taaggggatg gctggcctga
    11281 tataactgct gcgcgttcgt accttgaagg attcaagtgc gatataaatt ataaagagga
    11341 agagaagagt gaataaatct caattgatcg acaagattgc tgcaggggct gatatctcta
    11401 aagctgcggc tggccgtgcg ttagatgcta ttattgcttc cgtaactgaa tctctgaaag
    11461 aaggggatga tgtagcactg gtaggttttg gtacttttgc cgttaaagag cgtgctgccc
    11521 gtactggccg caacccgcag accggtaaag agatcaccat cgctgctgct aaagtaccga
    11581 gcttccgtgc aggtaaagca ctgaaagacg cggtaaacta agcgttgtcc ccggtgggga
    11641 tgtgacgaag ttcaagggcg catctactga tgtgcctttt ttatttgtat tcggtgactt
    11701 tctgcgtctt gtgggctgac aattgccccc gtttcttgtc acaataggcc tttgcgcgca
    11761 tcgatacgtt gcgtgaggta cacagtcatc tacagcggag tgttgttaca ccatgatgga
    11821 cagcttacgc acggctgcaa acagtctcgt gctcaagatt attttcggta tcattatcgt
    11881 gtcgttcata ttgaccggcg tgagtggtta cctgattggc ggaggcaata actacgccgc
    11941 aaaagtgaat gaccaggaaa tcagccgtgg gcagttcgag aacgccttta acagcgagcg
    12001 taatcgcatg cagcaacagc tgggcgatca atactccgag ctggcagcga atgaaggcta
    12061 tatgaaaacc ctgcgtcaac aggtgctgaa tcgtctgatc gacgaggcgc tgttggatca
    12121 gtacgctcgt gagctgaaac tgggtatcag tgatgagcag gttaaacagg cgattttcgc
    12181 gaccccagcc ttccaggttg acggcaaatt tgataacagc cgctataacg gtatcctcaa
    12241 ccagatgggg atgaccgccg atcagtacgc ccaggcgctg cgtaaccagc tcactaccca
    12301 acagctgatt aacggtgttg ccggtaccga ttttatgctg aaaggtgaaa ccgacgagct
    12361 ggcggcactg gtcgcgcaac aacgcgtggt gcgtgaggcg actatcgatg ttaacgcgct
    12421 ggcggcgaag cagcctgtga ccgaacagga gattgccagc tactacgaac aaaacaaaaa
    12481 caatttcatg acgccggaac aattccgcgt gagttacatc aagctggatg ccgcaacgat
    12541 gcagcaaccg gttagcgatg cggatatcca gagctactac gatcagcatc aggatcaatt
    12601 cacccagccg cagcgtaccc gctacagcat catccagacc aaaactgaag atgaagcgaa
    12661 agcggtactt gatgagctga ataaaggcgg tgattttgct gcattagcca aagaaaaatc
    12721 tgccgatatt atctctgccc gtaacggcgg cgatatgggt tggttagaag atgccactat
    12781 ccccgacgaa ctgaaaaatg ctggtctgaa agaaaaaggg caactgtctg gtgtcatcaa
    12841 atcttcggtc ggtttcctga ttgtacgtct ggacgacatt cagccagcga aagtgaagtc
    12901 gttagacgaa gtgcgtgacg atgtcgcggc gaaagtgaaa cacgaaaaag ccctcgatgc
    12961 gtactatgcg ctgcagcaga aagtgagcga tgcggcaagc aacgacaccg agtctctggc
    13021 cggtgcagag caagcagccg gcgttaaagc cactcagacg gggtggttta gcaaagataa
    13081 cctgccggaa gagttgaact tcaagccggt tgccgacgct atctttaacg gcggtctggt
    13141 aggtgaaaac ggcgcgccag gcatcaactc tgacatcatc accgtagatg gcgaccgcgc
    13201 attcgtgctg cgcatcagcg agcacaaacc ggaagtggtg aaaccgttgg cagatgttca
    13261 ggaacaagtt aaggcactgg ttcagcacaa caaagctgaa caacaggcga aagtggatgc
    13321 tgagaaactg ctggttgatt tgaaagccgg caaaggtgcg gaagctatgc aggctgccgg
    13381 tctgaaattt ggcgagccga aaaccttaag ccgttccggt cgtgacccga ttagccaggc
    13441 ggcgtttgca ctgccactgc cagcgaaaga caaaccgagc tacggtatgg cgaccgatat
    13501 gcaaggtaat gtggttctgc tggcgctgga tgaagtgaaa caaggttcaa tgccggaaga
    13561 tcagaaaaaa gcgatggtgc agggtatcac ccagaacaac gcacaaatcg tctttgaagc
    13621 tctgatgagt aacctgcgta aagaggcgaa aatcaaaatt ggcgatgcgc tggaacagca
    13681 ataatcctga agccgcctcg caaaaaaatg cgttgcagct gtaacaagaa aaggtcgctt
    13741 tcgcggcctt ttccatttct gaacattgcc atttgtttac tgttttcact gccgttaagg
    13801 tgattccact gttaacaaac aaggagaaaa cagtatgaaa cacggaatta aagcactgct
    13861 cattaccctg tccctggcct gtgccggaat gtctcatagc gcgctggcgg cagcttctgt
    13921 ggcgaaaccg acggcggtag aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc
    13981 agtaccggcg aaagccagtg acgaagaagg cacccgggtc agcattaata atgccagcgc
    14041 ggaagagcta gcccgcgcga tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag
    14101 ttatcgcgaa gagtacggtc cgtttaaaac ggtggaggat ctaaagcagg tgccggggat
    14161 gggcaattcg ctggtggaac gtaatctggc ggtattaacc ctgtaattaa tttgcatagt
    14221 ggcaattttt tgccagactg aagaggtcat accagttatg acctctgtac ttataacaac
    14281 aacgtaaggt tattgcgcta tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt
    14341 ttaccaacac gtcaacaacg cccgctacct tgaatttctc gaagaagccc gctgggatgg
    14401 gttggaaaat agcgacagtt ttcagtggat gacggcccat aacatcgcct tcgtcgtggt
    14461 caatatcaat attaactatc gtcgcccagc ggtattaagc gacctgttaa ctattaccag
    14521 tcagttgcag caattaaacg gtaaaagcgg catcttaagc caggtcatta cactggagcc
    14581 ggaagggcag gtggtagcgg atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca
    14641 gaaagcatta gctctggaag gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac
    14701 gttttgtggt gccggatgct caagccgcat ccggcgacac ccggaataat tacctcaacc
    14761 cggttttctg cttcatcgct gccatcaccg tcggtttatc ggccagataa tgattcaaac
    14821 cgttggcgcg taaattacat gccgcacaat gaccgcaacc gtcgccttta atgccgttat
    14881 agcaggtcaa cgtttcgtta cggactaaat ccagtttgcc gtaataatct gccagcgccc
    14941 aggtttccgc tttatcaatc cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc
    15001 ccaaactgac ggcatggttt agtgctttca caaactcatc gcggcaatcc gggtagccgg
    15061 agaaatccgt ttcacagacg ccggtaatta cggcttctgc ttttacctga tacgcatata
    15121 ttgccgccag cgtcaggaac aaaatattac gccctgggac aaacgtattc gggatgccat
    15181 cggcttcagg ttcataatca ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca
    15241 gctcgttgag cagcgttaca tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca
    15301 gttcgcgtgc cacgtcgatt tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat
    15361 ggacttcatc atattgttgt aatgcctgca ccagacaggt ggtggaatcc tggcctccac
    15421 tgaacacaac gacagcacgt ttcatagata atcctgcttg aacaataaaa gcgttatggt
    15481 aacgcctgcg attaacccgg accagctatt cattgcgacg gcgcgggaag ccaggcttcg
    15541 gtaaattcaa accagccgcg cggtgtcagt cgcacaccgt tcacgccggg aggggcacta
    15601 atgcgatagt gatagttgaa cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa
    15661 accgctttca gggcattaaa tcggttttcc tcatcaggca ttacttgcac cgcgtccagc
    15721 gtcgattgca gatgtgcata tgctggagcg tcgaaaacat gtggccatag tggatcgcaa
    15781 cgtagccatt gctccagagt atattccggt gcttcgccaa ttaatctgtc gcccatcatg
    15841 aggtctgcgt gtgccagtag ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt
    15901 gtgagttcac agccttccgc tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt
    15961 tctatcggta agtgataaac cagcgtcaag gttttcggta gtttgacttc atccggtact
    16021 tgccagtgcg gaatagtcca gcctggcagt aatgcatgac tggcggtgat caggttttct
    16081 ccgacttcta acgtttgtaa taaaccggat tgatgaataa tggagatcac ttttcgcgcc
    16141 tgccagaggg agagtcgggg acttttgcgc aacgttaaat agcaaaaacc taaactgatg
    16201 ccactactga cctggctgac ccgttgcagc tcctccggtt tgccgatggt gatttgcacg
    16261 ggatgccgac aactggttcc caaatctttt tcgaaaagcg gcggagttat ccagtactca
    16321 accgctttaa gcagcggatg acgcaaatgg taataatcat ggctctccag gcgcaccagc
    16381 tccgctgtga attgtgttaa gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc
    16441 gccagatggc tgcaatagct cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa
    16501 aagaaggtca gacactgcgg atgggtgact tcaatacgct tcacgctaat aaataattga
    16561 tccagtgctg gcagttgtaa cagcatcaat aatcgctggt gtaagtgtga ggcttttact
    16621 gcatcgccgt tatgccagtg tagggttgaa cgaagataaa agtcccagcg taacccgtca
    16681 gtagaggttt cccagtgatg cgctaaatcg ccaatcgggc gctgagtatt attatcgaag
    16741 cgggtcaggc cggaaaatat ctgcccggcg agatgctgct cggcacggcc gggcaaaaag
    16801 cctggttgta gcggttcgag cgggcgatag tagggaatac gcaacgtggg tgtatcgttt
    16861 tgccattgtc cgcccataaa cggctgtaac agagtgcgca gctcacctgg ggccagttgc
    16921 gccagctcca gcacatcttg ctgctttccg gtttccagtg cctgttccat catcgcattg
    16981 cgtagcgatt ccggcgtgac cagaaagcgt aattgtccgc gctttccgcg tcctgactgc
    17041 gcctgccact ccagccatcc cgcctcctgt gcctgacgca acagcgtacg aacatggcgt
    17101 tcgctgcaaa aacagcgttc ggccagttcg ctgacggtga cggtttgcgg ctttccggct
    17161 gaaggttgcc acagacgttg atactggtta agacggttga gcaatcgcat ataaacccgg
    17221 aacaatatta tttaactatt cactattact tccgtatata tcaggtgata ctcaatcacc
    17281 attaaccgtg tcacagagtg gagaaagaaa tggctcgtct ggcagcattt gatatggatg
    17341 gcactttatt gatgcctgac catcatttag gtgagaaaac cctctctact ttggcgcgac
    17401 tgcgtgaacg cgacattacc ctcacttttg ccacggggcg tcatgcgctg gagatgcagc
    17461 atattctcgg ggcgctatcg ctggatgcat atttgattac cggcaacgga acgcgcgtgc
    17521 attctctgga aggtgaactt ttacatcgtg atgatttacc tgcggatgtc gcggagctgg
    17581 tgctgtatca gcaatgggat acccgagcca gcatgcatat cttcaatgac gacggttggt
    17641 ttaccgggaa agagatccct gcgttgttgc aggcatttgt ctatagcggt tttcgttatc
    17701 agataatcga tgtcaaaaaa atgccactcg gcagcgtcac caagatctgc ttctgtggcg
    17761 atcacgacga tcttacacgc ttgcagatcc agctatacga agcattaggc gagcgtgcac
    17821 atttgtgttt ttccgccacg gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg
    17881 ctgcattgac ggtgctgacc caacatttag gtttatcgtt gcgcgattgc atggcctttg
    17941 gtgatgcgat gaacgatcgc gaaatgttag gcagcgtcgg tagcggattt attatgggca
    18001 atgcgatgcc gcaactgcgc gcggagctcc cgcatttacc ggtgattgga cattgccgaa
    18061 atcaggctgt ctctcactat ttgacgcact ggctggacta tccacatcta ccttattccc
    18121 ccgaataacg agatcccttc cagcaccggg caattgcccg gttttttttg cgttgagttt
    18181 gtcattttgt gccatggtgt ttaaaccgca cagaataaat tgtcgcgatt tcacctttaa
    18241 aatagaatta aaagagaaaa aaattctctg tggaagggct atgttagata aaattgaccg
    18301 taagctgctg gccttactac agcaggattg caccctctct ttgcaggcac tggctgaagc
    18361 cgttaatctg acaaccaccc cctgctggaa gcgcctgaaa cggctggagg acgacggtat
    18421 ccttatcggc aaagtcgccc tgctggatcc ggaaaaaata ggcctcggcc tgaccgcctt
    18481 tgtgctgata aaaacgcaac atcacagcag cgaatggtat tgccgctttg tcacggtggt
    18541 taccgaaatg ccagaagtgc tggggttctg gcgcatggct ggtgaatacg attatctgat
    18601 gcgcgtccag gttgccgaca tgaaacgcta cgacgagttt tataagcgtc tggtaaacag
    18661 cgtgccgggg ctgtcggacg tcacttccag cttcgcgatg gaacagatta aatacaccac
    18721 ttctttaccc atcgaataaa tatccagaat caggtcagga cacaacgcgt gcgattattt
    18781 gctcaattaa gctggtattt ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg
    18841 cttgtcatta tcgcgatgct gcaactggtt ccgccaaaag tggttggtat tgttgtcgat
    18901 ggcgtgacag aacaacactt tactaccggg cagatcctga tgtggatcgc caccatggtg
    18961 ctgattgccg ttgtggttta tctcctgcgt tacgtctggc gcgtattgct gtttggtgcg
    19021 tcttatcaac tggctgttga actgcgtgaa gattattacc gtcagctaag ccggcagcat
    19081 cctgagtttt acctgcgtca tcgcacgggt gacctcatgg ctcgtgcgac taatgacgtc
    19141 gatcgcgtcg tgtttgccgc cggagaaggg gtgctgacgc tggtggattc actggtaatg
    19201 ggctgcgctg tgttgattat gatgtctacg caaattagct ggcagttgac cttatttgcc
    19261 ctgttaccga tgccagtgat ggcgatcatg attaagcgca acggcgatgc cttgcatgaa
    19321 cgctttaagc tggcacaggc ggcgttttct agtcttaatg accgcaccca ggaaagcctc
    19381 accagtattc gcatgatcaa agcctttggt ctggaagatc gccagtcggc gttatttgcc
    19441 gcggatgccg aagataccgg caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc
    19501 gacccgacca tctatatcgc gattggtatg gcgaacttgc tggcgattgg cggcggtagt
    19561 tggatggtgg tgcagggcag tttaacgctg ggccagctca ccagttttat gatgtattta
    19621 ggtctgatga tttggccaat gctggcgctg gcatggatgt ttaacattgt ggaacgtggt
    19681 agtgctgcgt acagccgtat tcgcgcgatg ctggcggaag cgccggtggt gaacgatggt
    19741 agcgaacccg tgccggaagg gcgtggcgaa ctggatgtaa atattcacca gttcacgtat
    19801 ccgcagactg accatcctgc gctggaaaac gtcaatttcg ccctgaaacc cggtcagatg
    19861 ctgggtattt gcgggccgac tggttccggc aaaagtaccc tgttgtcgct cattcagcgt
    19921 catttcgacg tcagcgaggg ggatattcgc tttcatgata ttcctctgac gaagttacaa
    19981 ctcgatagct ggcgtagccg cctggcggta gttagccaga caccgttcct tttttccgac
    20041 accgtagcga acaacatcgc gttgggttgc ccgaatgcca cccagcaaga gattgagcat
    20101 gtcgcgcggt tagccagcgt acatgacgat attttgcgtt taccgcaagg ttacgataca
    20161 gaggtgggcg agcgcggcgt gatgctttcc ggcgggcaaa aacagcgtat ctctattgct
    20221 cgtgcgttat tagtcaacgc ggaaatcctc atccttgatg atgcgctttc ggcggtggac
    20281 ggacgcacag agcaccagat cctgcataac ctgcgtcagt gggggcaggg aagaacggta
    20341 atcatcagtg cccatcgcct ttcggcactg acggaagcca gtgaaattat tgtgatgcag
    20401 cacggacata tcgcccagcg tggcaatcat gatgagctgg cgcaacaaag cggctggtat
    20461 cgcgatatgt atcgctatca acaactggag gcggcgctcg acgacgttcc ggaaattcgc
    20521 gaggaggccg tcgatgcgta gttttagcca actgtggccg actctcaagc gtctgttagc
    20581 gtacggttcg ccgtggcgga aaccgctggg gattgcggtc ctgatgatgt gggttgcggc
    20641 agcggcagaa gtcagtgggc cgctgcttat cagctatttt atcgacaata tggtagcgaa
    20701 aaataacctg ccgttgaaaa tggttgcagg gctggcggcg gcgtatgttg ggctacaact
    20761 gtttgccgcc gggctacatt acgcgcagtc gctgctgttt aatcgggcgg cagtaggcgt
    20821 agtgcaacag ttgcgtaccg acgtgatgga tgctgcgtta cgccagccat taagcgagtt
    20881 tgatacccaa cccgtcgggc aggtgatttc ccgcgtcact aatgacactg aagtgatccg
    20941 cgatctctac gttaccgtag tggcaactgt cctgcgtagc gccgcactgg tgggcgcgat
    21001 gctggtggcg atgttcagcc tcgactggcg aatggcgctg gtggcgataa tgattttccc
    21061 ggtggtgctg gtggtaatgg tgatatacca gcgttacagc acgccgattg tccgtcgtgt
    21121 gcgcgcctat ctagcggata tcaacgacgg ctttaacgaa atcatcaatg gcatgagcgt
    21181 tatccagcag ttccgtcagc aggcgcgatt tggcgaacgt atgggggagg ccagtcgttc
    21241 acactatatg gccaggatgc aaaccctgcg cctcgacggt tttctgctgc gtccgctgct
    21301 gagtctgttt tcatcgctca ttctttgtgg cttgttgatg ctgtttggct tctccgctag
    21361 cggcaccatt gaagtgggcg tgctgtatgc gtttatcagc tatcttgggc gacttaacga
    21421 accattaatc gaactgacca cgcaacaggc gatgctgcaa caggctgttg ttgctggtga
    21481 gcgcgtgttt gaactgatgg acggaccgcg ccagcaatat ggcaatgatg atcgcccgtt
    21541 acagagtggc accatcgaag tcgataacgt gtcatttgct tatcgcgatg acaatctggt
    21601 gctaaagaac attaatctct ctgtgccttc gcgtaacttt gtggcactgg tcgggcatac
    21661 cggcagtggc aaaagcaccc tcgccagttt attgatgggc tattacccgc taacggaagg
    21721 tgagattcgc cttgatggtc gtccattaag ttcgctaagt cacagcgcgc tgcgccaggg
    21781 cgtggcaatg gtgcagcaag atccggtggt gctggcggat accttcctcg ccaacgtgac
    21841 gctggggcgt gatatctccg aagaacgcgt ctggcaggcg ctggaaaccg tgcaactggc
    21901 ggagctggcg cgcagcatga gtgatggtat ttatacgccg ctgggcgagc aggggaataa
    21961 tctctccgtc gggcaaaagc aactgctggc actggcacgc gtgttggtgg agacgccgca
    22021 aatcctgatc cttgatgagg caaccgccag cattgactcc ggtactgaac aggcgattca
    22081 acatgctctg gcggctgtgc gtgaacatac cacgctggtg gtgattgctc accgcttatc
    22141 gaccattgtt gatgccgaca ccattctggt gcttcatcgt gggcaagccg tggagcaggg
    22201 cactcaccag caactgctgg cggcccaggg acgctactgg cagatgtatc aactgcaact
    22261 tgcgggcgaa gagctggcag ccagcgtgcg tgaagaagaa tcattgagcg cctgaatagc
    22321 gcaatatttc atcgttggtg caaaaatgta acgcactgtg cactgtcata gtgcgttttc
    22381 attttcaatc ttcttaactt cctgctctct ttctcgtttt tcatttctgg cacaccgctt
    22441 gcaatacctt cttcgtgtag cagaaccatt accgaattct gaccggaggg gatctatgaa
    22501 gctggtgacc gtgataatca aaccattcaa gctggaagac gttcgtgaag cgttatcttc
    22561 cattggtatt cagggcctga ccgtcaccga agtgaaaggt ttcgggcgtc agaaagggca
    22621 tgccgagctg taccgggggg cggaatacag cgtcaacttc ctgccaaaag taaaaattga
    22681 tgtggcgatt gctgatgacc aactcgatga agtgatcgat atcgtcagta aggcggctta
    22741 caccggaaaa attggcgacg gcaaaatctt cgtcgctgaa ttgcaacgcg tcattcgtat
    22801 tcgtaccggc gaagccgacg aagcggcgct gtaatctctg gcacacagca acaggaacga
    22861 aaaatgaaga tagcgacgat aaaaactggg cttgcttcac tggcgatgct gccgggactg
    22921 gtaatggctg cacctgcggt ggccgataaa gccgacaatg cgtttatgat gatttgtact
    22981 gcgctggtgc tgtttatgac tattccgggg attgccctgt tttacggtgg gttgattcgc
    23041 ggcaaaaacg tgctgtcgat gctgacgcag gtgacggtga cgtttgcact ggtctgtatt
    23101 ctctgggtgg tttacggtta ctcgctggcg tttggtgagg gcaacaactt cttcggcaac
    23161 attaactggt tgatgctaaa aaacatcgaa ctgacggcgg tgatgggcag catttatcag
    23221 tatatccacg tggcgtttca gggctcgttt gcctgcatta ccgtcggctt gatcgttggg
    23281 gcgctggcgg aacgaatccg cttctcagct gtgttgattt tcgtggtggt atggctgacg
    23341 ctctcttaca ttccgattgc gcatatggtg tggggcggtg gtttgctggc ttctcacggt
    23401 gcgctggatt tcgcgggtgg cacggtggtg catattaacg ccgcaatcgc cggtctggtg
    23461 ggcgcgtatc tgataggcaa acgcgtgggc ttcggcaaag aggcgtttaa accgcacaac
    23521 ctgccaatgg tcttcaccgg tactgccatt ctctatattg gttggtttgg ctttaacgcc
    23581 gggtcagcgg gcacggcgaa tgaaatcgcg gcactggcat ttgtgaatac tgtggtcgca
    23641 acggcggcgg caattcttgg ctggatcttc ggtgaatggg cgctgcgtgg taagccttct
    23701 ctgctgggag cgtgttctgg cgcgattgcc ggtctggtcg gcgtgacgcc agcgtgtggc
    23761 tacattgggg ttggcggcgc gttgattatc ggcgtggtag ctggtctggc gggcttgtgg
    23821 ggcgttacta tgctcaaacg cttgctgcgg gtggatgatc cctgcgatgt cttcggtgtg
    23881 cacggcgttt gtggcattgt cggctgtatc atgactggga tttttgccgc cagttcgctg
    23941 ggtggcgtgg gcttcgctga aggtgtgacg atgggccatc agttgctggt acagctggaa
    24001 agcatcgcca ttacgatcgt ctggtccggt gtggtggcat ttattggcta caaactggcg
    24061 gatctgacgg ttggtctgcg tgtaccggaa gagcaggagc gagaagggct ggatgtcaac
    24121 agtcacggcg agaatgccta taacgcgtaa caagcactgc aaaaaatagc cggacggttt
    24181 tacctccggc tattttttta attgtgatta cgcatcaccc cttcctgaac ggtcgaggca
    24241 accagcacgc cgtcctgggt ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc
    24301 gaggtgctct ccacgctata cagcagccat tcattcaaat taaacgggcg atggaaccac
    24361 atggaatggt caatggtggc aatctgaatc cccggttcga gaaaaccgat gccgtgcggc
    24421 tgtagagcta ccggcaggaa gttaagatca gaagcgtaac cgagcagata ctgatgaacg
    24481 cgcaggtcat ccggcacgct accatttgcg cggatccaca cctgacgatg tggttctgcg
    24541 acgtgacctt tcagtgggtt atgaaactcc accggacgga cttccagcgg acgatcgcag
    24601 atgaatttat ctttaagcac tggcggcagc aggtgcgcca gcgattgggc gatttgcgtt
    24661 tccgaaggca ggccatcagg cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc
    24721 gcttctggtg cctggaaaga ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca
    24781 gcaacccggc gggcgctgaa gctgttaccg tcacgcagcg tttcgacatc ataaataatc
    24841 ggcttcttac tatcgccagg gcgaagaaag tagctgtgaa acgaatgtac cagccgctct
    24901 tcagggacgg tctcttttgc agcatacaag gcctgaccca cgacctggcc gccaaacacc
    24961 tggcgtaaac ctaaatcttc actctggccg cgaaagagtc cttcctcaat tttttccaga
    25021 tttaacaatg tcagtaaatt ttttagcgcc tgactcatat aactctccag taacaaagct
    25081 gccgcagcaa gccaaagtga gttgagtata acgcaaattt gctactggtc cgatgggtgc
    25141 aatggtctga attacgggct aattacaggc agaaatgcgt gatgtgtgcc acacttgttg
    25201 acgttactat tttgttaacc actcttccgg cgaggaaagt tagcccgctg gtgcattgat
    25261 aataaggaga aatgaatgaa actcgtgcac atggccagtg gtttagcggt tgcgattgcg
    25321 ttggcggctt gcgcagataa aagcgcggat attcagacgc cagccccggc tgcaaataca
    25381 tctatttcag caacacaaca acctgctatc cagcaaccga atgtctccgg taccgtctgg
    25441 atccggcaga aagtcgcact gccgcctgat gctgtgctga ccgtgacact ttctgacgcg
    25501 tcgttagccg atgcaccgtc aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa
    25561 cagtcaccat tcagctttgt tctgccattt aatccggcag atgttcagcc gaacgcgcgt
    25621 attctgttga gtgcggcgat taccgtgaat gacaaactgg tatttatcac cgataccgtt
    25681 cagccggtga tcaaccaggg cggaactaaa gccgacctga cattggtgcc ggtacagcaa
    25741 accgccgtgc cggttcaggc cagcggtggt gcaacgacta ccgtaccttc gacttcacca
    25801 actcaggtga atccgtcttc ggcagttcca gctcctacgc aatattaagc gacgttaacc
    25861 ctctccgccc ggagagggtt agtagttcca gcgataacgc tgcaagtcga tttgcccgct
    25921 tcccgatacc atcacacctt ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc
    25981 ggttagcgaa attgtgccgt ggcgattaac cacccggtgc cagggtaagg tgctgccttc
    26041 agggagacgc tttaacacac cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt
    26101 cgccacatca ccgtaagtgg tgacatagcc ttcgggaata gcggcgacga tttgccagac
    26161 gcgttgggga aatgaatctt ctttttccat cttttcttcc tgaggtaatt tttcagcata
    26221 atctggaaaa acgcccgagt gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat
    26281 gggttgcaat tagccggggc agcagtgata atgcgcctgt gcgttggttc tcaacgctct
    26341 caatgggggc tctgttggtt ctcccgcaac gctactctgt ttaccaggtc aggtccggaa
    26401 ggaagcagcc aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc ccccacccat
    26461 ttctgcctcc caccgtttcg tcaaaaaacc ccaacatggc taaactttaa ccacaactga
    26521 cgtcgcaaga attgtctggc tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt
    26581 gatggttttg tggttgccgt tcctgccgat aaaaaggatg cctatcggga aatggccgct
    26641 aaggctgcgc cattgtttaa agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc
    26701 gatgtaccgg atggcaaagt gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa
    26761 gaggttgtct ttagctggat tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa
    26821 aagatgatgt cggacccacg gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag
    26881 cgaatgatct atggcggatt cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg
    26941 cggcgtgccg tcagcccaaa aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct
    27001 ttcggtaatg ccttgctgta gagccagccc tgaccgtagt gcacgccatg ctggcgtagc
    27061 cactcttctt gtttactggt ttcgattccc tccgctacca ttttcagttt cagtgttttt
    27121 gccatttcga tgatatgcgg cgtgacattt ttatattcca gcgcatcaac gaaagattta
    27181 tcgatcttca gaatgtcgac atccagatcc tgtaaataac ttaaacttga atatcccgta
    27241 ccaaaatcat caagataaat ttcatggccc gcctcccggt agcgagaaat tatcggggcg
    27301 ctggttttcg gatcggcaaa ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta
    27361 acctgatagt gattgatcat ttcacgcagc aattgcggga ttttttccga ggtgagcacg
    27421 gtggattcaa gattgatcga aatatgctgc tgtggatgct gacgcagcca gtcgcccata
    27481 tcttcaaaga cgcttcttat aatcagtagc gtcaatggct cagaaaggcc cgtttgctgt
    27541 gccagcggaa taaaactatc tggtgacaac caactaccgt ctgtctgcgg ccagcgcgcc
    27601 agtgcctcag caccgacaat tttgccattg gctaaggaga caatcggctg atagtgcacg
    27661 caaatatcac gattttcgat agcatcctgc agccgatgat gcggtgactg gatacggcgc
    27721 aaaatacgca gcacaaacat cgctgccagc aggccaatca ccaacccgag cggtaaccaa
    27781 ataaagactt gccgatgcca gcctttctgc aacattttcg ttgaagccca ggtgatgatc
    27841 gaaatattca tctccgggaa gggcaggata tcgtaaatga ttccattatt ttcgatatgc
    27901 tcaccgggtg ttttttgtag cctggtaata attccctgag caatttcatc gctgctggtt
    27961 atgacaacgt tatgggcatt gccaataatg gcggcatcaa tttgccatga gctataggga
    28021 atgacatcaa taaaggaagc ggggtcgatc atgacgacat aatgtgccgt tcccatggcg
    28081 accatgtaac ggataatgcc taaatcgtta tgcgatgtta accagacacg ataaccatct
    28141 ttcgaaattt tacctggctc ggggaaggta tcgggcggac tttcatgctc cagagacgaa
    28201 cactggggaa cgttgttatc gatataaacc acttcctgaa tatagcgata actgtaagat
    28261 acccgacgca tttccatgag atgggcttcg ctacaggcag cgccttgcca tctttccagc
    28321 tcttgcagcg catctttccc ttgtgtcgcc accttattgg cccgaatagc gacgcgggag
    28381 gaataggtat ccagcttttc aataaacgat gtttctacct gctgatgggc cagccagatg
    28441 cttaagccga caggcagcaa tactgaaaga atcagtactc ccgaaataag gccgaccaga
    28501 tgtcgtgttc tcaccagcat atccttatta acacagtgta gggaaagtat atctgattgt
    28561 ggaaaaaggc tggcaggaga taaggaggta gagctggtat ttcatgaagt gatgggtgga
    28621 aacgatcatt gtcatttgac caggcgcaga gctttacgcc tggtatgtaa ccctaactta
    28681 agatgttgat tcggcgcgag cgagggtaaa aatatcgtaa aaagacagtt cacctttacg
    28741 gacgatcatt ttttgcagtt gttccttatg aacatccgcg acacgcggag agttcaagat
    28801 ggcttcaacc agcgcggcag gtacaaagcg cgtgttgtac cattcgccgt tatagcaaac
    28861 gcggaaatcc agcacgtcga tatcttcata acggtagcgt gggtagacat cgaaaaagaa
    28921 gaaaccatta agcaggataa ataatacaac cagtagccag acagagcctg acagcgtttc
    28981 ggactgcaac atcaccgcca gggtggcaaa aaaagcaacg tacataccga taaacagccc
    29041 cggatgttta cggataaaac tgatactaaa gcggggtttg ttgtcgcgtt tttcgcgggt
    29101 attcagagac tcaatcgttt cggtcagcag gcgttgtatt tcggtcatcg tttgccttcg
    29161 tggtatctgc aactttccag ggaacaccct attttaacgt gggaatagta gaggggaagt
    29221 aacagattta ccacaatttt gcataacagt tgcgaaaaac cgattacaat tttttaatta
    29281 ttctggctgg attaccgccc acgacaacgt tgttcgggac atcttttgtg acaactgcac
    29341 ctgaggctac cacgacgtta tcaccaatgg tcacaccagg gttaatgacc gcgcgtccgc
    29401 caatccagac gttattaccg atggtgacgg gtttccccag ttcagcaccg ctattacgtg
    29461 ctacagggtc gatgggatgt gttgccgtgt agatatgaac gcctggtgcc aacatacagt
    29521 tatcaccgat gcgaataggg cagacatcaa gcatcacgca atcgaagttg gcgaaaaaat
    29581 tattaccgag aaaaatgtta tagccatagt cacagcgaaa cgttggctca atataagcct
    29641 ctgtcacctg accgaataga tcagcgagaa tttgctggcg taatgtgtgc tcttccgcca
    29701 gggaatgatt gtatcggtga ataagctgac gagcgcgcag gcgatcgcga gataacgtct
    29761 catctgccga gcgatacaac tcaccagcaa tcatcttttc tttttctgtg ctcattactg
    29821 acctccggtt gctgaaaatg cggcaacaca gtaagacgaa aagtcagtga agagaacgtt
    29881 cccattacat ctgtgtgata aagatcacat agggtatgtc ttcatggcga aaaagtataa
    29941 aattcttaat aaacagccgg ttatagctcc gaaagcgaat tatttattag cgaataaatt
    30001 tccatactga ggaagggatc ttgtcgtaca gtttattcat ggtcaattcg gcgaggcggt
    30061 gatctgcggc tgagtaaaat accgccagtt cattatctga taattcgtat ttatttttct
    30121 cgataacacg ctccagcgtg tcaattgtct ggcaacgacg taaacgcatt aaataatcgg
    30181 ttttcgttaa aggtttttcg gacatacttc tacccatggt tgtaataatt ctaacaagat
    30241 aaactcgcag gattctcttt cgtcgcattg acaaaacaac ggaatagtcg attacctgac
    30301 ttccgccatt tctgaagatc ctgcatatta ataccataac tactgaacaa cataaaggtg
    30361 tcatccagat attcgtcgat ctgctcaatg agcttattgt cttcattata cttaattttg
    30421 taattaagtg cgaaggtcgc aatatgctca atcagttcat tcaactggag gttgatcgcc
    30481 gaggttgggt cgttaaccca gccatgattg ctttcttcaa ggtttgcaag gcagtcatga
    30541 tacagggttt cacagagaaa cttaagctgt gcgatatcat gtcttttggg tgagtattca
    30601 tccataacgc gtccccttct tagcggttga actaacggac acctttcggg atggaaaaaa
    30661 cttactgacc tggacttgcc ctctcgtttg ttagcttaac tataagccac tctttgcagg
    30721 ttttcatcgc attattacga aaaattacaa ttaggaagga atcgggacag gactcacttt
    30781 ttcatactag cactaacccg cagcaggtaa aagcagtctg ctcgaactgt tcataacatt
    30841 tcaggattat cgccagatga aattggtgac actaatacca ggattgctct gaatatgacg
    30901 taataaccga ggaatgaata aagaattacc gcaaataatt aagaatagcc tctaaatgat
    30961 tatggattat taaatctatt agcgctactt aatataaatt aactaacaat cagtattttt
    31021 tatgaatttt atccgtggtt aatactggtt ttcgtatgag atcctgagtt ggtggttcaa
    31081 ttactcctta atgttcgtag gttatgcata aaaaaggccg cttgcgcggc cttagtgatt
    31141 acacgttgta tcaatgatga tcgacagtat ggctgtgctc gatatcttca ttcttgcggc
    31201 taaagcggcg gcgaaccaca acaaagaata ccggaacgaa gaagattgcc agtaccgttg
    31261 cggtcaccat cccgcccatt acaccggtac ctactgcgtt ctgcgcgccg gaaccagcac
    31321 cagtactgat aaccagcggc ataacgccga ggataaacgc cagcgaggtc atcaggatcg
    31381 gacgtaaacg catccgcact gcatcaagcg tcgcttcaat cagaccttta ccttctttat
    31441 ccatcaagtc tttggcgaat tcgacgataa gtatcgcgtt cttcgccgac aacccaatgg
    31501 ttgtgagcag gcctacctgg aagtaaacgt cattggtcag gccacggaag gtggcagcca
    31561 gcaacgcacc gataaccccc agcggaacga ccagcataac ggagaacgga atcgaccagc
    31621 tctcgtacag cgccgccaga cacaggaata cgacaatcaa cgaaatcgcg tacagtgaag
    31681 gtgcctggtt gccggagagg cgttcctgat aggacatccc cgtccagtca tagccaacac
    31741 cggtaggcag tttgctcgcc agttgttcca ttagctccat tgcttcaccg gtacttttac
    31801 ccggtgccgc ctggcctaag atttccatgg atggcaggcc attgtaacgt tccagacgcg
    31861 gcgaaccgta ctcccaacga gaagaggaga atgccgagaa cggcaccatc tgaccatcag
    31921 cagcacgaac ataccagtcg ccgatatcat ccggcagcat acggtatttc gcttctgaca
    31981 tgacgtaaac tttcttcaca cgaccgcggt cgataaagtc gttcacatag ctaccgcccc
    32041 atgcagcgcc cagagtggtg ttaatgtcgt tgatagaaac acccagcgcc tgcgcttttt
    32101 cctggtcgat atcaatctta aactgcgggg tatcttccag accgtttgga cgtacgctgg
    32161 tcagcatatc agggtgcttc gctgcttctg caagcagctg gttacgcgcc tgagtcagtt
    32221 tttcgtgacc aaggccagcc tggtcaatca gctcaaagtc aaagccggtt gcagtaccca
    32281 gttccacgat tgcgggcagg ttaaaggcga aaaccatcgc atctttgatt tgcgagaaag
    32341 cgcgtgttgc acgcatggta atcgcttcaa ctttgttttc ttcgcccgga cgatcggccc
    32401 agtccttcaa ggaaacgaac gcaataccgg tattctgacc acgtcccgca aagccgaagc
    32461 cgttaacggc gaacaccgac tcaacgttgt tcttttcttt ggtcagatag taatgcgtta
    32521 cctcattgag cactttctgc gtacgttcct gcgttgcacc tgctggcagc tgaaccatgg
    32581 tcataaatac gccctggtcc tcatctggca agaaggagct tggcagacgc acgaacagat
    32641 aggccatgcc gaccacgatg atcagataca gcaccaggta acgccccgta ctgcgcagaa
    32701 taccgcctac gctgtcggtg tagtggtgcg tgctcttctc gaacatgcgg ttaaaccagc
    32761 cgaagaagcc ttttttacct tccccgtgat cgcctttggc aatcggtttc agcatggtgg
    32821 cacaaagagc cggagtcagg atcaacgcta ccagtaccga cagcgccatt gctgaaacaa
    32881 tggtaataga gaactgacga tagatagcac cagtagaacc gccaaagaag gccatcggta
    32941 cgaataccgc cgacagtacc atcgcgatac cgaccagagc gccctgaatc tgccccatcg
    33001 acttacgggt agcttctttt ggcggcaaac cttcttccgc cataacacgc tccacgtttt
    33061 ctaccacaac gatagcgtca tccaccaaca agccgatagc gagcaccatc ccgaacattg
    33121 ttagcgtgtt tatcgagaag ccaaaggcgg caaggacggc aaaggtcccg agcaatacca
    33181 ccggtacggc aatggtcgga atcaacgtcg cgcggaagtt ctgcaggaac agatacataa
    33241 ccaggaacac gaggatgatc gcttcgacca gcgttttaac cacttcgtga atagagattt
    33301 tcacgaacgg cgtggtgtcg tacgggtaaa caattttcag acccgacggg aagaacggtt
    33361 ccatcttcgc cagttcagca cggattgccg cagcggtatc cagcgcgttt gcaccggtcg
    33421 ccagcttgat ccccagaccg gaagccggtt ggccgttaaa ctctgcgatg atgtcgtagt
    33481 tctcaccacc cagctcaatc ttcgccacat cacgcagcag tacgcgggaa ccatcttgat
    33541 tcactttcag caggattttg ccgaactctt cagtagaggt cagacgcgtc tgagcaataa
    33601 tagaggcgtt aagctgttgg cctttcaccg gcggcgtacc accgagctga cccgccgcaa
    33661 cctgggcgtt ctgcgctttg atggcggtaa tgacatcaac cggcgttagc tggaatttgt
    33721 tcagctcatt cgggttcatc cagatacgca tcgcgtactg tgaaccgaac aactgaacat
    33781 cacccacgcc cgacgtacgg ctgatggcat ctttcatatt cgccgctacg tagtcagaga
    33841 tatcctcctg cgtcatggtg ccatcggtgt tgataacgcc gacaaccatc aggaagctgc
    33901 tggatgattt ctcaacgctc accccttgct gctgaacttc ttgcggcagc aacggcatcg
    33961 ccagctgcag tttgttctgc acctgaacct gcgcgatatc cgcatcagta ccagactcaa
    34021 aggtcagggt gatctgcacg gtacccgtgg agtcactgtt agaggacatg tacatcaggt
    34081 tatcgatacc gttcatattc tgttcgataa cctgtgtcac cgtgtcctgc actgttttcg
    34141 catcagcgcc ggggtaggag gcggagatcg ttactgccgg cggtgcaatc gtaggatatt
    34201 gcgccaccgg cagtttgagg atcgccagcc cccctgccaa catgatgata atggcgatca
    34261 cccacgcaaa aatcgggcga tcgataaaga aattaggcat gtcttaacgg ctcctgttta
    34321 agttaagact tggactgttc aggctgagca ccgcttgcgg cttgctggtt attatcagcg
    34381 gtaacttctt gtgcttttac ctggacacca ggacgcactt tctgcagccc acttattact
    34441 acgcgatcgc ctgctttcag accttctgtc accagccact tatcgccaat agcctggctt
    34501 gcaacgatcg gacgggtttc cactttgtca tccgcgccaa ctaccagtac ggtggcatca
    34561 ccacgcggcg tacgggttac gccctgttgc gggactaaaa tagcgtttgg attaagccct
    34621 tcttccagac gtgcgcgcac gaacataccc ggcagcagag tgtgatccgg gttcgggaag
    34681 atagcgcgta gggtgataga cccagtggtc tgatcaacgg taacgtcaga gaattccagc
    34741 gtaccgtcct gcgggaactt aatgccgtca ctggtgatca gcgacacttt ggctttgccg
    34801 ttctcttgtt tcagcgtgcc attcgccagt tcctgtttca ggcgcaggaa gtcgttgctg
    34861 gactgggtca catcaacgta gatcggatca agttgctgca cggttgccag cgcagtcgcc
    34921 tgaccgttct gtaccaatgc gccttccgtc acgttcgact taccaatgcg accgctaatc
    34981 ggagaggtga ctttggtgta agccagattg atccgcgcag tttcaacggc agctttcgcc
    35041 gcagttaccg cagcattcgc ctgttgcgca tcagccagag cctgatcgta ctcttgctta
    35101 ctgatgtact gagtaccgag cagtttctga taacgattca ccgtcaattg cgcgatattg
    35161 gctgcagcct gggctttcgc cagatcacct ttcgcactgt cgtatgtcgc ctgataggtc
    35221 gcaggatcaa tctgatagag agagacacct gcttcgatgt cgctaccttc tttgaaatta
    35281 cgcttcagga taatcccgct aacttgagga cgaacttctg cgatccggta ggcactggtg
    35341 cgacccggaa gctcggttgt gatctgcaga ggttcagttt tgactgttac tacgccaacg
    35401 gcgggcatct gctggccacc ttgttgggcc tgtttgtcgt cacatcctgt tagggctaag
    35461 ctgcctgaga gcatcagaac gaccgccaga ggcgtaaacc ctctgttttt gttcatatgt
    35521 aaacctcgag tgtccgattt caaattggtc aatggtcaaa agttaataaa cccattgctg
    35581 cgtttatatt atcgtcgtgc tatggtacat acattcacaa atgtatgtaa atctaacgcc
    35641 tgtaaattca cgaacatatg gcacgaaaaa ccaaacaaga agcgcaagaa acgcgccaac
    35701 acatcctcga tgtggctcta cgtcttttct cacagcaggg ggtatcatcc acctcgctgg
    35761 gcgagattgc aaaagcagct ggcgttacgc gcggtgcaat ctactggcat tttaaagaca
    35821 agtcggattt gttcagtgag atctgggaac tgtcagaatc caatattggt gaactagagc
    35881 ttgagtatca ggcaaaattc cctggcgatc cactctcagt attaagagag atattaattc
    35941 atgttcttga atccacggtg acagaagaac gacgtcgatt attgatggag attatattcc
    36001 acaaatgcga atttgtcgga gaaatggctg ttgtgcaaca ggcacaacgt aatctctgtc
    36061 tggaaagtta tgaccgtata gaacaaacgt taaaacattg tattgaagcg aaaatgttgc
    36121 ctgcggattt aatgacgcgt cgggcagcaa ttattatgcg cggctatatt tccggcctga
    36181 tggaaaactg gctctttgcc ccgcaatctt ttgatcttaa aaaagaagcc cgcgattacg
    36241 tcgccatctt actggagatg tatctcctgt gccccacgct tcgtaatcct gccactaacg
    36301 aataaccctg aatctgactc caggattttt cctggacatt ttcgtcgttg ctattctggt
    36361 tcactgcgtc gtgatattct tgcggtttga ctttttcagg tcgttcttca ggttcagaaa
    36421 ccttcattca tcatgactat gttccagtat tacaaacgat cacggcattt tgttttttca
    36481 gcatttattg cttttgtttt tgtcttgtta tgccagaaca cggcgtttgc gcgggcgtca
    36541 tcgaatggtg atctgccgac aaaagcggac ctgcaggcgc aacttgactc actaaataaa
    36601 caaaaagatc tttctgctca ggacaaactg gtgcagcagg atctgacaga tacattagcc
    36661 accctcgata aaatcgatcg cataaaagaa gagacagttc agctacggca aaaagtcgct
    36721 gaagcgccgg aaaaaatgcg ccaggcgacc gcggcgttaa cagcacttag cgatgtcgat
    36781 aacgacgaag aaacgcgcaa aattctgagt acgctttcgt tgcgccagct ggaaactcgc
    36841 gttgcccagg cgctggacga tttgcaaaac gcacaaaacg atctggcgtc ttataacagc
    36901 cagctggttt cgttacagac gcagcccgaa cgcgtgcaaa atgcgatgta taacgcttcg
    36961 cagcagctgc aacaaattcg cagtcgtctg gatgggactg atgtcggcga gactgactta
    37021 cgtcccagcc agaaagtgtt aatgcaggct cagcaggcgt tgctgaatgc ggagattgac
    37081 cagcagcgta aaagcctgga agggaacacc gtcttgcagg ataccttgca aaagcaacgt
    37141 gattacgtga cggcgaacag cgcccgtctg gagcaccagt tgcagctgtt gcaagaagcg
    37201 gtaaacagca agcgcctgac tttaactgaa aaaacggcgc aggaagccgt ctccccggat
    37261 gaagccgcgc gtattcaggc taatccgctg gtgaaacagg aactggaaat taaccagcag
    37321 ttaagtcagc gtctgattac cgcgactgaa aacggtaatc agttgatgca gcaaaacatt
    37381 aaagtcaaaa actggctgga gcgggcgctg caatcggaac gcaatattaa agagcagatt
    37441 gccgtcctga agggcagcct gctgttgtct cgtatccttt accagcaaca acaaacgctg
    37501 ccctcggcgg atgaactgga aaacatgacc aaccgcatcg cggatttgcg tctcgaacag
    37561 tttgaagtta accagcagcg tgatgcactc ttccagagcg atgcgttcgt caacaaactg
    37621 gaagaaggtc acaccaacga agtcaacagc gaagttcacg atgcgttatt gcaagtggtt
    37681 gatatgcgtc gcgaattgct ggatcaactc aacaaacagt tgggtaacca gctgatgatg
    37741 gccattaacc tgcaaatcaa ccagcagcag ttaatgagcg tatcgaaaaa cctgaaatcc
    37801 atcctgactc agcaaatctt ttgggtgaac agtaaccgtc caatggactg ggactggatc
    37861 aaagcgttcc cgcaaagcct gaaagatgaa tttaagtcga tgaaaatcac ggtgaactgg
    37921 gaaaaagcct ggcctgccgt ttttatcgct ttcctcgctg gtttgccgct gctgttgatt
    37981 gccgggctga tccactggcg tctgggctgg ctgaaagcgt atcaacagaa actggcttcc
    38041 gctgtgggtt ccctgcgtaa cgatagccag ctcaacacac caaaagcgat ccttatcgac
    38101 ctgatccgcg cgctgccggt gtgcctgatt attctcgcgg ttggcctgat tctgttgacc
    38161 atgcagctca acatcagcga actgctatgg tcgttcagta aaaaactggc gattttctgg
    38221 ctggtgtttg gcctgtgctg gaaggtactg gagaaaaatg gcgttgccgt gcgtcacttc
    38281 ggcatgccgg agcagcagac cagccactgg cgtcggcaaa ttgtccgcat cagtctcgcg
    38341 ttgctgccta tccatttctg gtctgtggtg gcagaacttt ccccgctgca tctgatggat
    38401 gatgtgctgg ggcaggcgat gattttcttc aacctgctgc tgattgcctt cctggtatgg
    38461 ccaatgtgcc gcgaaagctg gcgtgataaa gagtcgcaca ccatgcgact ggtcaccatt
    38521 accgtgctgt cgattatccc gattgcattg atggtgctga ctgccacggg ttatttctac
    38581 accacgctgc gcctctccgg gcgttggatt gaaaccgttt atctggtgat catctggaac
    38641 ttgctctacc agacggtatt gcgcggctta agcgtagcgg cgcggcgtat cgcctggcgt
    38701 cgtgcgctgg cgcgtcggca gaatctggtg aaagagggcg cagaaggtgc cgaaccgccg
    38761 gaagaaccca ccattgcact ggagcaagtt aaccagcaga cgctgcgtat taccatgttg
    38821 ctgatgtttg cgctgttcgg tgtcatgttc tgggcaattt ggtccgattt gatcaccgtg
    38881 ttcagctatc tcgacagcat cacgctctgg cattacaacg gcactgaagc tggcgctgcg
    38941 gtggtgaaaa acgtcaccat gggcagtctg ttgtttgcga ttatcgcctc aatggtggcc
    39001 tgggcgttga ttcgcaacct gcctggttta ctggaagtgc tggtgctctc gcgactgaat
    39061 atgcgtcagg gcgcgtcgta tgccattact accatcctta actacatcat tattgccgtt
    39121 ggtgcgatga cggtgttcgg atcgctgggc gtctcttggg ataaactcca gtggctggcc
    39181 gcagcattat ccgtaggtct tggttttggt ttacaagaaa ttttcggtaa cttcgtctcc
    39241 ggtttgatca ttctgttcga acgtccggtg cgtattggcg atacggtaac cattggtagc
    39301 ttctcgggga cggtaagtaa gatccgtatt cgtgcgacaa cgattaccga tttcgatcgc
    39361 aaagaagtga tcatcccgaa caaagcgttt gttaccgagc gtctgatcaa ctggtcgttg
    39421 actgacacta ctacgcgtct ggtgatccgt ctcggcgtgg cctatggctc cgatctggaa
    39481 aaagtgcgta aagtgttact gaaggcggcg actgagcacc caagggtgat gcacgaacca
    39541 atgccggaag tcttctttac ggcgtttggt gccagcacgt tggatcatga gctgcgtctg
    39601 tatgtgcgtg aactgcgtga ccgtagtcgt actgtcgatg agctgaaccg tactatcgat
    39661 cagctgtgcc gtgaaaacga catcaacatt gcctttaacc agcttgaagt gcatctgcac
    39721 aacgagaagg gcgatgaggt gacggaagtc aaacgcgact acaaaggcga tgacccgacg
    39781 ccagcggtag ggtaataacc aaagggggcg acatttagtt gccccgagat tactgacaaa
    39841 gtgcgcgttg ttcatgccgg atgcggcgtg accgccttat ccggcctacg aaaccgcaag
    39901 aattcaatgt attgcaggag cggtgtaggc ctgataagcg tagcgcatca ggcagttttg
    39961 cgtttgccca caatcttagg ggacatttag cggccccatt tatttctcac ttttccgcct
    40021 catcatcgcg cgttaatttc ttttcataat cgcgttttac aatatccagc gcgcgcagca
    40081 cggtgcgggc tgggatctga ttttcttcca gcagcacaat caaatcgaca gccagtttga
    40141 catcgtcagg ggcattttcc agtgacatac tctctccatt gctagcgggt taagcgcgct
    40201 agcctgtttt cgattttttc cagcgcatgg cggcagcgtg ccaggcgcgc ttcataggct
    40261 tccacttcac gatgcagcgt ttgctgttcc acgagatcgg tcacccgcgc caggcgggct
    40321 ctgcgttcgg caaccatctc acgtagccgc cgctcaaaat cctgatgctg aatacgttta
    40381 cgctgccagc gggcaatttt cggtggcgca ctgtcccact cgcgcaatga ccaggcggag
    40441 gcttcacgcg cgatggcttc cagttgtgcc gccagatgtt ccgccagcca ggccacttgc
    40501 ggcagctgtt gctgctcaac tgcatgacga agcgcagcca gattatcgcc cgcctcatcg
    40561 agacaagcct gcagtgttgt cgcacgggtt tgaaaaaggt gtctgtcaaa acgggcactt
    40621 agcgtggcaa attgcgctac cggggcacaa cgctgacgca gcgtagcgag ctgtccttcc
    40681 agtttttcca gcagcagggc ggttttcacg ataagacctc aatgaaaatg ataattgtta
    40741 tgctaaagta gccactctgt aagctgacaa cactcaatat gcaacgaatc attttaatca
    40801 tcattggctg gctggcggta gtgctgggta cgctgggcgt ggtattaccg gtattaccga
    40861 cgacgccgtt tatcctgctg gcggcctggt gctttgcccg ttcttccccg cgctttcacg
    40921 cctggttgct gtaccgctca tggtttggca gctatctacg tttctggcag aaacatcatg
    40981 cgatgccgcg cggcgtcaaa ccgcgggcga ttttgcttat tttgctcacg tttgccattt
    41041 ctctgtggtt cgtccagatg ccatgggtgc gcatcatgtt gctggtaatt ctcgcctgtt
    41101 tgcttttcta tatgtggcga attccggtga ttgatgaaaa gcaagaaaag cactgaagca
    41161 caacaatcgc agttgcaatt attgcgtaca gccagtacat tctggcgttt tcgagcacag
    41221 gcgcaggcgg tcaaaggtta aacaactgtt acttttgata cgtttaaaac gcgccgtgag
    41281 taccaccgta acaagcaggc atacacttat gaccgcgact gcacagcagc ttgagtatct
    41341 caaaaatagc atcaaaagca ttcaggacta cccaaaaccc ggcattcttt tccgcgatgt
    41401 caccagctta ctggaagacc cgaaagctta cgctctcagc atcgacttgc tggttgagcg
    41461 ttacaaaaat gcgggcatta ccaaagttgt cggcaccgaa gcgcgtggct tcttgtttgg
    41521 cgctccggta gctctgggtc tgggcgttgg ctttgtaccg gtccgtaaac cgggcaaact
    41581 gccgcgtgaa accatcagtg aaacttacga cctggaatac ggcaccgatc agctggagat
    41641 ccacgttgat gccatcaaac cgggcgacaa agttctggtg gtggacgatc tgctggcaac
    41701 cggcggcact atcgaagcga ccgttaaact gatccgtcgt ctgggtggtg aagtggctga
    41761 cgctgcgttc attatcaacc tgttcgatct cggcggcgaa cagcgtctcg aaaaacaggg
    41821 cattaccagc tacagccttg tcccgttccc gggccattaa ttatcgccag tcttgtgctg
    41881 cccacgctac ggacagcaca agatgtgcat tcagcctcgc cgttctgacg gggctgtgtt
    41941 agcattaccc cttcgtgaat ccaccttcca gcgtttcaga gcctgccaat gagttatcag
    42001 gtcttagccc gaaaatggcg cccacaaacc tttgctgacg tcgtcggcca ggaacatgtg
    42061 ctgaccgcac tggcgaacgg cttgtcgtta gggcgtattc atcatgctta tcttttttcc
    42121 ggtacccgtg gcgtcggaaa aacctctatc gcccgactgc tggcgaaggg gctaaactgc
    42181 gaaaccggca ttaccgcgac gccgtgcggc gtgtgcgata actgtcgtga aatcgagcag
    42241 gggcgctttg tcgatctgat tgaaatcgac gccgcctcgc gcaccaaagt tgaagatacc
    42301 cgcgacctgc tggataacgt ccagtacgct ccggcgcgtg gtcgtttcaa agtttatctg
    42361 atcgacgaag tgcatatgct gtcgcgccac agctttaacg cactgttaaa aacccttgaa
    42421 gagccgccgg agcacgttaa gtttctgctg gcgacgaccg atccacagaa attgccggtg
    42481 acgattttgt cgcgctgttt gcaatttcat ctcaaggcgc tggatgtcga gcaaattcgc
    42541 catcaacttg agcacatcct caacgaagaa catatcgctc acgagccgcg ggcgttgcaa
    42601 ttgctggcgc gcgccgctga aggcagcctg cgagatgcct taagtctgac cgaccaggcg
    42661 attgccagcg gtgacggcca ggtttcaacc caggcagtca gtgcgatgct gggtacgctt
    42721 gacgacgatc aggcgctgtc gctggttgaa gcgatggtcg aggccaacgg cgagcgcgta
    42781 atggcgctga ttaatgaagc cgctgcccgt ggtatcgagt gggaagcgtt gctggtggaa
    42841 atgctcggcc tgttgcatcg tattgcgatg gtacaacttt cgcctgctgc acttggcaac
    42901 gacatggccg ccattgagct gcggatgcgt gaactggcgc gcaccatacc gccgacggat
    42961 attcagcttt actatcagac gctgttgatt ggtcgcaaag aattaccgta tgcgccggac
    43021 cgccgcatgg gcgttgagat gacgctgctg cgcgcgctgg cgttccatcc acgtatgcct
    43081 ctgcctgagc cagaagtgcc acgccagtcc tttgcgcccg tcgcgccaac ggcagtaatg
    43141 acgccaaccc aggtgccgcc gcaaccgcaa tcagcgccgc agcaggcacc gactgtaccg
    43201 ctcccggaaa ccaccagcca ggtgctggcg gcgcgccagc agttgcagcg cgtgcaggga
    43261 gcaaccaaag caaaaaagag tgaaccggca gccgctaccc gcgcgcggcc ggtgaataac
    43321 gctgcgctgg aaagactggc ttcggtcacc gatcgcgttc aggcgcgtcc ggtgccatcg
    43381 gcgctggaaa aagcgccagc caaaaaagaa gcgtatcgct ggaaggctac cactccagtg
    43441 atgcagcaaa aagaagtggt cgccacgccg aaggcgctga aaaaagcgct ggaacatgaa
    43501 aaaacgccgg aactggcggc gaagctggcg gcagaagcca ttgagcgcga cgcgtgggcg
    43561 gcacaggtga gtcaactttc gctaccaaaa ctggtcgaac aggtggcctt aaatgcctgg
    43621 aaagaggaga gcgacaacgc agtatgtctg catttgcgct cctctcagcg gcatttgaac
    43681 aaccgcggtg cacagcaaaa actggctgaa gcgttgagca cgttaaaagg ttcaacggtt
    43741 gaactgacta tcgttgaaga tgataatccc gcggtgcgta cgccgctgga gtggcgtcag
    43801 gcgatatacg aagaaaaact tgcgcaggcg cgcgagtcca ttattgcgga taataatatt
    43861 cagaccctgc gtcggttctt cgatgcggag ctggatgaag aaagtatccg ccccatttga
    43921 tcgtaagcac agcttacgtt cgtcatcctt aacgtgattg agagagaaac ctatgtttgg
    43981 taaaggcggt ctgggtaacc tgatgaagca agcccagcag atgcaagaaa aaatgcagaa
    44041 aatgcaggaa gagatcgcgc agctggaagt caccggcgaa tctggcgcag gtctggtaaa
    44101 agtgaccatc aacggtgcac acaactgccg tcgcgtagag atcgacccga gcctgctgga
    44161 agacgacaaa gagatgctgg aagacctggt ggctgcagca ttcaacgacg cagcacgtcg
    44221 tattgaagaa acgcagaaag aaaaaatggc ctctgtatcc tccggaatgc agctgccgcc
    44281 tggctttaag atgccgttct gatgcaaacc agcccgctgt taacacagct tatggaagca
    44341 ctgcgctgtc tgccgggcgt tggcccgaag tcggcgcagc gtatggcgtt cacgctgctt
    44401 cagcgcgatc gtagcggcgg gatgcgtctg gcgcaggcgc tcacccgggc gatgtcggaa
    44461 atcggccact gcgccgattg ccgcactttc accgaacagg aagtctgtaa catctgttcg
    44521 aatccgcgtc gtcaggaaaa cggtcaaatc tgtgtggtgg agagtccggc ggacatctac
    44581 gccattgagc agacggggca gttttcaggc cgttattttg tgttgatggg gcacctgtca
    44641 ccgctggacg gcatcggtcc ggatgatatc ggccttgatc gtctggaaca gcgtctggca
    44701 gaggaaaaaa tcactgaagt gatcctcgcc accaatccca cggttgaagg tgaagctacc
    44761 gctaactaca ttgccgagct ttgcgcgcaa tatgacgtgg aagccagccg aatcgctcat
    44821 ggcgtaccgg ttggcggcga actggaaatg gtcgatggca ccacgctgtc acactccctt
    44881 gccgggcgtc ataagattcg tttttaagca aacgagagca ggatcacctg ctctcgcttg
    44941 aaattattct cccttgtccc catctctccc tcatcctgtt tttaacctta aaatggcatt
    45001 attgaggtag acctacatga aaggacaaga aactcgtggt tttcagtcag aagtgaaaca
    45061 gcttctgcac ctgatgatcc attctctcta ttccaataaa gaaatcttcc tgcgtgagct
    45121 tatctctaac gcctccgatg cggcggacaa gctgcgtttc cgtgcgctat ctaacccgga
    45181 cctgtacgaa ggtgatggcg aactgcgcgt tcgtgtctct ttcgataaag acaagcgcac
    45241 gctgaccatc tccgataacg gcgtggggat gacccgcgac gaagtgattg accatctggg
    45301 gactatcgct aaatccggta ccaaatcatt cctcgaatcc ctgggttctg accaggcgaa
    45361 agacagccag ctgatcggtc agtttggtgt tggtttctac tctgcgttta tcgtggccga
    45421 caaagtgacc gtgcgtactc gcgcggcagg cgaaaaacca gaaaacggcg tcttctggga
    45481 atcggctggc gaaggtgaat ataccgttgc cgacatcacc aaagaagatc gtggtactga
    45541 aatcaccctg catctgcgtg aaggcgaaga cgagttcctc gatgactggc gcgtgcgttc
    45601 catcatcagc aaatactccg accatatcgc gctgccggta gagatcgaaa aacgcgaaga
    45661 gaaagacggc gaaaccgtta tctcctggga gaaaatcaac aaagcgcagg cgctgtggac
    45721 tcgtaacaag tcggaaatca ccgatgaaga gtacaaagag ttctacaaac acatcgccca
    45781 cgactttaat gatccgctga cctggagcca caaccgcgtt gaaggtaagc aggagtacac
    45841 cagcctgctg tacatcccgt cccaggctcc gtgggatatg tggaaccgtg atcataaaca
    45901 cggcctgaaa ctgtacgtcc agcgtgtgtt catcatggac gacgcagaac agttcatgcc
    45961 gaactatctg cgcttcgtgc gtggtctgat tgactccagc gatctgccgc tgaacgtttc
    46021 ccgtgaaatc ctccaggaca gcacggtaac gcgtaacctg cgcaatgcgc tgaccaagcg
    46081 tgtgctgcaa atgctggaaa aactggcgaa agacgacgcg gaaaaatacc agaccttctg
    46141 gcaacagttt ggcctggtac tgaaagaagg tccggcggaa gatttcgcta accaggaagc
    46201 gatcgccaaa ctgctgcgtt ttgcttctac ttataccgat tcttctgcgc agaccgtgtc
    46261 tctggaagac tacgtttccc gcatgaaaga agggcaggag aaaatctact acatcactgc
    46321 agacagctat gcggcagcga agagcagccc acacctggaa ctgctgcgta agaaaggcat
    46381 cgaagttctg ctgctttccg accgcatcga tgagtggatg atgaactatc tgactgagtt
    46441 cgacggtaaa ccgttccagt cggtgtctaa agttgacgag tcgctggaaa aactggctga
    46501 cgaagttgat gagagcgcga aagaagcgga gaaagcactg actccgttca tcgaccgtgt
    46561 gaaagccctg ctcggcgagc gcgtgaaaga tgtccgtctg actcaccgtc tgaccgatac
    46621 gccagcgatc gtttcgaccg acgcggacga aatgagcact cagatggcga aactgttcgc
    46681 tgcggcgggc cagaaagtgc cagaagtgaa atacatcttc gaactgaacc cggatcacgt
    46741 actggtgaaa cgtgcggcag atactgaaga tgaagcgaag ttcagcgagt gggtagaact
    46801 gctgctggat caggcgctgc tggcagaacg cggcacgctg gaagatccga acctgtttat
    46861 tcgtcgtatg aaccagctgc tggtttcctg atgtcatgcc ggatgacctt tgtgtcatcc
    46921 ggcatttttc ttttcatcat ctgcactttc cgcaaattat ctcgccatta accgtttcag
    46981 cctcaggtgc ctttcttgag gcaatcgcct gttggtggta tcgtttatcg ctttttcaaa
    47041 aaattcgaca cattttaagg ggattttcgc aatgcgtatc attctgcttg gcgctccggg
    47101 cgcggggaaa gggactcagg ctcagttcat catggagaaa tatggtattc cgcaaatctc
    47161 cactggcgat atgctgcgtg ctgcggtcaa atctggctcc gagctgggta aacaagcaaa
    47221 agacattatg gatgctggca aactggtcac cgacgaactg gtgatcgcgc tggttaaaga
    47281 gcgcattgct caggaagact gccgtaatgg tttcctgttg gacggcttcc cgcgtaccat
    47341 tccgcaggca gacgcgatga aagaagcggg catcaatgtt gattacgttc tggaattcga
    47401 cgtaccggac gaactgattg ttgatcgtat cgtaggccgc cgcgttcatg cgccgtctgg
    47461 tcgtgtttat cacgttaaat tcaatccgcc gaaagtagaa ggcaaagacg acgttaccgg
    47521 tgaagaactg actacccgta aagacgatca ggaagaaacc gtgcgtaaac gtctggttga
    47581 ataccatcag atgactgcac cgctgatcgg ctactactcc aaagaagcgg aagcgggtaa
    47641 caccaaatac gcgaaagttg acggcaccaa gccggttgct gaagttcgcg ctgatctgga
    47701 aaaaatcctc ggctaattcg aaagcgcgca cggacagtcc cctcgccccc tcggggagag
    47761 ggttagggtg aggggaacag gcccgcacaa gcaaacttat cagcaatctc aggccggata
    47821 ttcattcggc cttttacaaa aacctcaatc tgcgctattc ctttttctga tttgacctct
    47881 cacagcaatt agttcttctt cctcactttt ccgctacaat tatcaacaag ttgaatcgat
    47941 aagaggcggt aatgcgtcag actaaaaccg gtatcctgct ggcaaacctg ggtacgcccg
    48001 atgcccccac acctgaagcg gtaaaacgct atctgaaaca atttttaagc gacagacgcg
    48061 tggttgatac ctcacggttg ttatggtggc cgttgctgcg cggcgtgatt ttgccgctgc
    48121 gctcgccgcg tgtggcgaag ctgtacgcct cggtctggat ggaagacggc tcgccgctga
    48181 tggtttacag ccgccagcaa cagcaggcgt tggcacaacg tttaccggat acgcccgtag
    48241 cgctgggcat gagctacggc tcgccatcac tggaaagcgc cgtcgatgaa ctcctggcag
    48301 agcatgtaga tcatattgtg gtgctgccgc tttatccgca attctcctgt tctacggtcg
    48361 gtgcggtatg ggatgaactg gcacgcattc tggcgcgcaa acgtagcatt ccggggatat
    48421 cgtttattcg tgattacgcc gataaccacg attacattaa tgcactggcg aacagcgtac
    48481 gcgcttcttt tgccaaacat ggcgaaccgg atctgctgct gctctcttat catggcattc
    48541 cccagcgtta tgcagatgaa ggcgatgatt acccgcaacg ttgccgcaca acgactcgcg
    48601 aactggcttc cgcactgggg atggcaccgg aaaaagtgat gatgaccttt cagtcgcgct
    48661 ttggccggga accctggctg atgccttata ccgacgaaac gctgaaaatg ctcggagaaa
    48721 aaggcgtagg tcatatacag gtgatgtgcc cgggctttgc tgcggattgt ctggagacgc
    48781 tggaagagat tgccgagcaa aaccgtgagg tcttcctcgg tgccggcggg aaaaaatatg
    48841 aatatattcc agcgcttaat gccacgccgg aacatatcga aatgatggct aatcttgttg
    48901 ccgcgtatcg ctaaagctga gcggtaaaga actgagcgcc gtcgcgaaga gcctcgtcgg
    48961 cggttttcat cattcgtgaa taatgcaaaa aggcatgcag cgtacctggg tagagtttga
    49021 actcacaggg ctgctgatgc gccgctaacg tctggtaaag cagacggctg tcatccagca
    49081 gcggatcgaa ctccgccccg gcaataaaac agggcggaac ttcgcgagtg agatcattat
    49141 taaacagaca gtaatacggt gactcgcggt ccgcgtcgtt gcttaaatac gcctcttcat
    49201 acatctgcaa atcctgttgc gttaagccat cccagacacc gcccaacaga cgacgagtca
    49261 cggaatcccg taatccgtaa agcccatacc acagcaaaac acccgcaatt ttaccgcaat
    49321 cgatctgttt atcacgcaac cacaacgcac tggcgagcgc cagcatggca cctgcggaat
    49381 caccggcaaa gccaatgcgg gacatattga tttgataatc ctccgcctgc tggtggaaat
    49441 aacaacaagc agccacaatt tcctctatcg cttgcggaaa acgcgcttca ggtgaaaggg
    49501 ggtaatcaat accaatcacc gtacattggc tgtagcttgc cagcaggcgc atgatgcgat
    49561 cgtgggtatc gagattgccg agaataaaac cgcctccatg caaataaaat agcgtcgccg
    49621 ggctatctgg ctgcggacaa aagagacgtg tttccacctg cccatatttt gttggaacca
    49681 tgtaagctct ggttgccatt tctggagcgc ccgcattcca gaatcggcgc tcaagcgtgt
    49741 aatactgtcg ttgctcagca atcgttcccg ttgcgggcca ggacggtaaa tccggctgaa
    49801 gagtattcac aacggtcttc atttcagcag aaataaggtc cagaacaggt agtttgtttt
    49861 ccggcttcat acaaaaactc ctttcaaatt acgtcattgt aaggaaccac tgccatgaaa
    49921 atgcgatccc gcctgctgat attgaaactg gctgcgtctc gcgcgctccc gtcagattgt
    49981 gttaacattc gccgctcagt taaccacccg taaaaacaac catgaaattt cccggtaaac
    50041 gtaaatccaa acattacttc cctgtaaatg cacgcgatcc gctgcttcag caattccagc
    50101 cagaaaacga aaccagcgcc gcctgggtcg taggtattga ccagacgctg gtcgatattg
    50161 aagcgaaagt ggatgatgaa ttcattgagc gttatggatt aagcgccggg cattcactgg
    50221 tgattgagga tgatgtagcc gaagcgcttt atcaggaact aaaacagaaa aacctgatta
    50281 cccatcagtt tgcgggtggc accattggta acaccatgca taactactcg gtgctcgcgg
    50341 acgaccgttc ggtgctgctg ggcgtcatgt gcagcaatat tgaaattggc agctatgcct
    50401 atcgttacct gtgtaacacc tccagccgta ccgatcttaa ctatctacaa ggcgtggatg
    50461 gcccgattgg tcgttgcttt acgctgattg gcgagtccgg ggaacgtacc tttgctatca
    50521 gccctggcca catgaaccag ctgcgggctg aaagtattcc ggaagatgtg attgccggag
    50581 cctcggctct ggttctcacc tcttatctgg tgcgttgcaa gccgggtgaa cccatgccgg
    50641 aagcaaccat gaaagccatt gagtacgcga agaaatataa cgtaccggtg gtgctgacgc
    50701 tgggaactaa gtttgtcatt gccgagaatc cgcagtggtg gcagcaattc ctcaaagacc
    50761 acgtctctat ccttgcgatg aacgaagatg aagccgaagc gttgaccgga gaaagcgatc
    50821 cgttgttggc atctgacaag gcgctggact gggtagatct ggtgctgtgc accgccgggc
    50881 caatcggctt gtatatggcg ggctttaccg aagacgaagc gaaacgtaaa acccagcatc
    50941 cgctgctgcc gggcgctata gcggaattca accagtatga gtttagccgc gccatgcgcc
    51001 acaaggattg ccagaatccg ctgcgtgtat attcgcacat tgcgccgtac atgggcgggc
    51061 cggaaaaaat catgaacact aatggagcgg gggatggcgc attggcagcg ttgctgcatg
    51121 acattaccgc caacagctac catcgtagca acgtaccaaa ctccagcaaa cataaattca
    51181 cctggttaac ttattcatcg ttagcgcagg tgtgtaaata tgctaaccgt gtgagctatc
    51241 aggtactgaa ccagcattca cctcgtttaa cgcgcggctt gccggagcgt gaagacagcc
    51301 tggaagagtc ttactgggat cgttaagtta tcgaccagac cggacgacga tggcatccgt
    51361 aggccggata agatgcgcta gcatcgcatc cggcaataac gtcttgtttt accccgtcac
    51421 cacctcacct gccggtggcg tttccagcaa ttccagcata gtacgggcaa tttcacgttc
    51481 acccatcact acctgattcg cgccacgttc ggtgatatac gtcacttcat cgtcataatg
    51541 ggcgcgggcg ataatctcaa tatccgggtt tttcgcgcgg gcagacgcca caatctcacc
    51601 cgcttcataa ccgttgggga tcgtcaggat cagccatttt gcacattcca gatgcgccag
    51661 ttgcataatt tcttcgttcg ctgcattgcc caatacagcg cggaccccac gctctcgcag
    51721 ctcatcaaca cgggttcgtg acgtctcaat caccaccagc ggaatatcag aggcgagcaa
    51781 tttctccccc agcaggctgc ctacacgacc gtaacccacc aatagcgcat ggttgcaaat
    51841 atccactggg atctgtttct cttcttcgat tgcctcttcc agcgtctgct cttccagcgt
    51901 ttcggtcttc gccagatatt tctccagtag tgcgaacagt accgggttga gcataatcga
    51961 caggatcgcc cctgccagta ccaggttttg tccggcctgc ggcagtaaat tcaatgccat
    52021 tcccagtccc gccaggataa acgcgaactc accaatctgc gccaggctgg cggcgatggt
    52081 taatgccgta cgttgggagt gaccaaacag tcgcaccagg aaaaatgcgg ctaacgactt
    52141 accaaacaga ataatcgcca gcgtcgccag cactgccagc ggttgctgaa tcagaattaa
    52201 cggatcaaac aacatcccga cggagacaaa aaacagcacc gcaaacgcgt cgcgcaatgg
    52261 cagcgtatcg tgggcggcac ggtgactcag ttcagactcg ttcagtacca tcccggcaaa
    52321 gaacgcaccg agtgcaaagg agacatcaaa cagctctacc gcaccaaagg caatccctaa
    52381 cgccagcgcc agcaccgaca gggtaaacag ctcgcgagaa ccggttgccg cgctgcgtgc
    52441 cataatccac ggcaccagac ggcgacctac cagcatcata atggcgataa atgcgatcac
    52501 tttgccgatg gtgatcccca tatcgactgc aagagtggca aagcccacat cgccctgttc
    52561 catcattcct gccactgcgg gcagcaacac cagcgtcaga accattacca ggtcttccac
    52621 aatcaaccaa ccgatggcga tttgcccacg ctgactgtca attaattgcc gttcttcaag
    52681 tgcgcgcagt aacaccacgg tactggcggt ggaaagacat aaaccgaaca cgataccggt
    52741 cattaacgac cagcccagca cggcagagag cgccataccc agcagcgtcg ccacggctat
    52801 ctgggcgatc gcaccgggaa tggcgatggc ctttaccgcc atcaaatcct tcagcgaaaa
    52861 gtgcaaaccg acgccgaaca tcagcaaaat aacgcccagt tcagccagtt ccggcgcaag
    52921 cttggtatcg gcaacaaagc ccggagtgaa tggtcctgcc agcacacccg ctaacagata
    52981 tcccaccaga ggagaaatac gtagtttatt ggccagcatg ccgaggataa aggcgagcac
    53041 aaggccgcca acaatggtgg tgataagcgg ggtggcgtga tgcattccgt ctccttttcc
    53101 tggtggttat tgtccatttt tggccgggga aaccaaaatt acaggtaata gtttatgaca
    53161 atttcattga tgatgttcat gaataattat tgaattttgc agaaaaatgg aattagctgc
    53221 aaaaaaagca cggatcggaa aacagaaggc ggtttaacag aggaaaggct tatggcgact
    53281 gtaaaacgat gcagccaaag tttgcctttg gctgcaatga aatcagtctt tatgccggtt
    53341 atcaggcagg aatatggtca acatccccaa tagtggcagg aaagcacaga ttttatagac
    53401 taactcgata ctggtgtgat cggcgataag ccccagaact gccgctccca gacctcccat
    53461 accaaaagca aaaccgaaaa agagtccaga aaccataccg atacgtcccg gaagcagctc
    53521 ctgagcgtaa accagaatgg cagagaatgc cgaagcgagg ataaatccaa taatcaccgt
    53581 taaaaccccc gtccagtgca ggctggcgta gggtaaaatc agcgtaaacg gcgcaacgcc
    53641 gaggatagag ccccaaatca catatttccg cccaatttta tcccctacag gcccgccgat
    53701 caccgtacct gccgcaacgg caaacaggaa ggcaaacaga tgaagctgag cattctggat
    53761 agataatccg aatttttgca tcagataaaa ggtgtaatag ctgctgatgc tcgccatata
    53821 gaaatatttc gagaaaatga ggattaacag aatgctgacc gccagtacca ctttgttgcg
    53881 cggcagtgga ttgataatcg tcgctttggg ttttccttta ttcattcggt gctgtgccga
    53941 gtaccaacgg ctgatttgcg ccaacaccac gatcgccagc agtgccgcaa gcacaaacca
    54001 ggcaacgttg cctttgccat aaggcgcgat aatcaccgcc gccagcaagg gtcccaggga
    54061 actgccaaag ttgccgccga cctgaaagat agattgcgcc aggccatgcc gcccgccgga
    54121 agccatacgg gctacgcgag aagattccgg atgaaagacc gatgaaccgg taccgaccag
    54181 cgccgccgcc agcagaactg cgccaaaact gcccgccagc gcaagcagca ccagaccact
    54241 taaggtaaag cacatgccaa ttggcaacga ccacggcatc ggatatttat cggtccagta
    54301 gccgaccact ggttgtagta gcgaagaggc gagctggaag gtgagggtta tcatgccaat
    54361 ctgcataaat gtcagagaaa attctgactg aagcagcgga taaatcgcca gaatcagcga
    54421 ttggatcatg tcgttcagca gatgtgagag gctgatagca cctaaaatac caaacgatgt
    54481 tcgggccttg gtcgttgacg cagccgcgcc cgccacaggc tggggttgtt cactcattgc
    54541 cataggaaag tcactttttc agggttgcga tgtaaagaat gatcttattt gtgattatta
    54601 cccgactaac atacctgtat gcgtcgcctg aaggaagtct caacgccgaa tacagaattt
    54661 ctaatctgga tgcagattta tcttcaccgg acgcagactt gtctatgatg tcgcgtcata
    54721 ctatttttca acacgttgaa atcaggtcag ggagagaagt atgaaattat tgcagcgggg
    54781 cgtggcgtta gcgctgttaa ccacatttac actggcgagt gaaactgctc tggcgtatga
    54841 gcaggataaa acctacaaaa ttacagttct gcataccaat gatcatcatg ggcatttttg
    54901 gcgcaatgaa tatggcgaat atggtctggc ggcgcaaaaa acgctggtgg atggtatccg
    54961 caaagaggtt gcggctgaag gcggtagcgt gctgctactt tccggtggcg acattaacac
    55021 tggcgtgccc gagtctgact tacaggatgc cgaacctgat tttcgcggta tgaatctggt
    55081 gggctatgac gcgatggcga tcggtaatca tgaatttgat aatccgctca ccgtattacg
    55141 ccagcaggaa aagtgggcca agttcccgtt gctttccgcg aatatctacc agaaaagtac
    55201 tggcgagcgc ctgtttaaac cgtgggcgct gtttaagcgt caggatctga aaattgccgt
    55261 cattgggctg acaaccgatg acacagcaaa aattggtaac ccggaatact tcactgatgt
    55321 cgaatttcgt aagcccgccg atgaagcgaa gctggtgatt caggagctgc aacagacaga
    55381 aaagccagac attattatcg cggcgaccca tatggggcat tacgataatg gtgagcacgg
    55441 ctctaacgca ccgggcgatg tggagatggc acgcgcgctg cctgccggat cgctggcgat
    55501 gatcgtcggt ggtcactcgc aagatccggt ctgcatggcg gcagaaaaca aaaaacaggt
    55561 cgattacgtg ccgggtacgc catgcaagcc agatcaacaa aacggcatct ggattgtgca
    55621 ggcgcatgag tggggcaaat acgtgggacg ggctgatttt gagtttcgta atggcgaaat
    55681 gaaaatggtt aactaccagc tgattccggt gaacctgaag aagaaagtga cctgggaaga
    55741 cgggaaaagc gagcgcgtgc tttacactcc tgaaatcgct gaaaaccagc aaatgatctc
    55801 gctgttatca ccgttccaga acaaaggcaa agcgcagctg gaagtgaaaa taggcgaaac
    55861 caatggtcgt ctggaaggcg atcgtgacaa agtgcgtttt gtacagacca atatggggcg
    55921 gttgattctg gcagcccaaa tggatcgcac tggtgccgac tttgcggtga tgagcggagg
    55981 cggaattcgt gattctatcg aagcaggcga tatcagctat aaaaacgtgc tgaaagtgca
    56041 gccattcggc aatgtggtgg tgtatgccga catgaccggt aaagaggtga ttgattacct
    56101 gaccgccgtc gcgcagatga agccagattc aggtgcctac ccgcaatttg ccaacgttag
    56161 ctttgtggcg aaagacggca aactgaacga ccttaaaatc aaaggcgaac cggtcgatcc
    56221 ggcgaaaact taccgtatgg cgacattaaa cttcaatgcc accggcggtg atggatatcc
    56281 gcgccttgat aacaaaccgg gctatgtgaa taccggcttt attgatgccg aagtgctgaa
    56341 agcgtatatc cagaaaagct cgccgctgga tgtgagtgtt tatgaaccga aaggtgaggt
    56401 gagctggcag taatccgaaa gtgccggatg tttgcatccg gcacaatgct taatcgcggc
    56461 gggcgatatc agcaaatttg gcatcgagga tctttgccag atcgcctgcc gccagttcga
    56521 tatccagtcc gcgcttgccg ccggaaacat aaatagtggc aaattcttgt gcgggggcgt
    56581 cgataatcgt tggcagacgt tttttctgcc ccagtgggct aatcccccca accaggtatc
    56641 ccgtcgaacg ctgcgcgacc atcggatcgg ccatctcaac tttcttggca cccagcgctt
    56701 ttgctacttt tttaagatcc agttgacctg cgaccggcgt aacggccacg gcaaggtgtt
    56761 tcatatcacc gttcactgcc accagcagcg ttttgtagac ctgatccgga ttcaaaccta
    56821 attttttgac gacttcatcg ccaaaattgg tttcagccgg atcgtgctcg taggtatgga
    56881 tttgaaacga aatcttgttt ttttcgagta atttaactgc gggtgtcatg gtaattttcc
    56941 tgccttaaac aaaaaaacga agcaagcata cgcctgatgg cggtccaaaa aaaagagtca
    57001 tcttgcctaa gagtattggc aggatggtga gattgagcga caatcgagtt acaccgtcgc
    57061 ttaaagtgac ggcataataa taaaaaaatg aaattcctct ttgacgggcc aatagcgata
    57121 ttggccattt ttttagcgca acatttgcgg caaattccct tctccataca ggtgtagtgc
    57181 accgaccgcg accacatatc gccccggcgg catggcgcgt aatttatccc gccaggcgag
    57241 atttcgctga tgcatcagca catcgtacag cgactgactg aacgtattgg gcagcgttat
    57301 atcattattt tgcggaggtg cattcagcca ccagctcatc atttgttgca gcaaccgtgc
    57361 gttggtatgc cagtgggtca gcgtatcgtc cagcagcgcc agtcctttgt cagggagctg
    57421 gagcaacatg gtaatctggt tttcagcccc ttccagttca atcacgggtt tatgttgttg
    57481 cttcgccgcc tgcaataact ggtaatcgat accgtattct gcccgcagcc ccagtttttg
    57541 tgcctgcgtc gcctgaagaa ccatcgcgat ttgccacagc ggttgagtag aaaagagcga
    57601 cggagaaatg cccatctcct ggctaatgtg ctgcagattt tggagttgct cctcgttaat
    57661 gcgctcttcc agcgcctcgc aagtaggcaa attagcaaaa ggcgtatcgc tggtggaaac
    57721 atccgcctcg acgatcagcg catcggcgtt tttgagcttt ttgagcaaac gggtgggcag
    57781 gggagccata tcgtggctac ccatatgaat actgccaatc agatgaaaat ggcgattgcc
    57841 gggaagggtg atatcgatgg caggccaggt gtaatgatta ccgcgcagcg cggcccaaag
    57901 cgtttttacc cggtacaaca gatccatacg acctcccttt gtgaaatatc atgctagcgc
    57961 gcggtgaggg atggcgcaaa tggagttggg gacttgttta atgtgtttgt atgattcagt
    58021 atgttcttgc atcgctattc acagggaagc aacagttaaa aaccatgaaa caggcaacaa
    58081 gaaaaccgac gacacccgga gatattcttc tctatgaata tctggaaccg ctcgatttga
    58141 aaatcaatga gttagcagag ttgctgcatg ttcatcgtaa tagcgtcagt gcactgatca
    58201 ataacaatcg taaactcacc actgagatgg cattccgtct ggcgaaagtt tttgatacca
    58261 cagtcgattt taggctaaac ctccaggcgg cagttgatct ttgggaagtt gaaaacaaca
    58321 tgcgcaccca ggaagaattg ggacggattg aaacagtggc tgaatatttg gcacgccgtg
    58381 aagagcgtgc aaaaaaggtc gcgtaaggcc aaaacatcgc ttcatctcgt aggccggata
    58441 aggcgtttac gccgcatccg gcagtcatgc gtcgatgcca aatgcgccgc cctaaagcag
    58501 cgcatccgca atgacgtact tattccttcg gtttaaaccg cagcaaccgg ttggcgttac
    58561 tcactacggt aatcgacgag agcgccattg ccgctccggc aactaccggg ttaagcagtg
    58621 ttccagtaaa cggccacaaa ataccggcgg cgaccggaat accgatgctg ttgtagataa
    58681 aggcaccgag caggttctgc ttcatgttgc gcagcgttgc gcgggaaatc gcgagcgcat
    58741 ccgcaacgcc catcaggcta tggcgcatca gggtaatcgc cgcggtttca atggcaacat
    58801 cactgccgcc acccatcgca atgccgacat ccgcctgggc cagcgctggc gcgtcgttaa
    58861 tgccgtcgcc caccattgcc acctgacgtc cttcactttg cagacgtttg atcgcttcgg
    58921 ctttaccgtc cggcagcact ccggcaatca cttcatcaat tccagcttct ttggcgatcg
    58981 cattggcggt ggttgggtta tccccggtca acatcaccag acgatatccc gctttatgca
    59041 ggcgttgcag cgccgccacg ctatcactac gcaacggatc gcgtacagcc agcagggcta
    59101 ctgctttccc gtcaatcgcc agcagcacag gcgttgctcc ttgcgatgcc tgagcagtaa
    59161 tctccgcttc gatagctttg gtaccaacct gttgctcatt taacagcgcc tgattgccca
    59221 gcaataacgc atgaccttca gcttcaccgc tcacgcccag cccgcgcaat gtgcggaaac
    59281 cgttgacctg cggcagctgc atatcacctg ctttatcgag gatcgcccgt gccagcggat
    59341 ggctggaacc ttgctccagt gccgccgcca gacgcaatgc ctgcgcttca tcaacatcag
    59401 caaacgtttt cactgcgaca acctgcggct tcccttcggt cagcgtcccg gttttatcga
    59461 acacgacggt atcgagtgta ctggagcgtt gcagcgcgtc ggcgtcccgc accagcacgc
    59521 caaactcagc cgcccgcccg acgccggaaa taatcgacat cggcgtcgcc agccccagcg
    59581 cacacggaca ggcgataatc agtaccgtgg tggcaatcac cagggtataa acaatttgcg
    59641 gtgccggacc aaagaagtac cagattgccg cactgaccag cgcaatcacc accaccacag
    59701 gtacaaatac ggctgagatt ttatccgcca gctgaccgat ttctggcttg ctgctctggg
    59761 cctggcgcac catgcgaatg atccgtgaca gcgtggtatg gctgccaacc gcactggcac
    59821 gaaacagcac gctaccgtcc tgtaccactg tcccggcatg gacgctttcg ccttcgcctt
    59881 tttgctgcgg gattggttcg cccgtcagca tcgcttcatc cagccatgct tcgccctggg
    59941 taatctcgcc atctaccggc acgcgatcgc cggtcgtcag gcgcagcaac atacctggct
    60001 gcacttccgc cagaggcacg cttttttcac cttcgtcagt aaccaggcgc gccgtcggtg
    60061 gggttaaatc gagtaatttc tccagcgcct tagaagaacg ctgacgtgcg cgtgcttcca
    60121 gcatatggcc gagattgatc agaccgataa tcatcgcgct ggcttcgtaa taaagatgtc
    60181 gcgcttccat cgggaaccac tgcggccaca ggttgacgct catcgaatag agccacgcca
    60241 cgccagtacc cagcgccacc agcgtatcca tcgtcgctgc accgttcagc aggcttttcc
    60301 atgcactgcg gtaaaaatgg ccgccggcga aaaccatcac cgccagggtt atcaggccga
    60361 taaccaacca caggctgcgg ttgtcagcgg tgaccatcat gttatcgcca atcatccccc
    60421 agaccatcac cgggataccc accgccagtg cgacaattgc ctgccagcgg aagcgcttca
    60481 tcgtagcaac ggcggtttcc tgctggcgct cacggcgttt cgcgtcatct tcaatcgctt
    60541 ccgcgccgta gcccgctttt tccaccgcct gcactaaatc ttgtggggag gcactgccca
    60601 tcaccagcgc agtacgctcc gccaggttta cccgtgcctg agtgacgccc ggtacgcttt
    60661 gcagcgcatt ttgtacgcgg gtgacacagc tggcgcagct catgccgctc agcagcaact
    60721 gctggctgtc atcgtcatcg gcggtcgctg ccggaagcgc ctcagaaacc gctgtcagtg
    60781 cttccgacgg gattgatgac tccgccagcg gtttagcctt tgggtggctt acagatgcgt
    60841 cataacccgc ttgtttgatg gtttcgatca gctgttctgc actggcagtc ccggtaacgt
    60901 gcgcttcagt gatagacaca tccgcttgtt ccacatccgg gcgctgttcg agactttctt
    60961 tcacgcgttt aacgcagtga ccgcaggaca ggccgtccag ggtcaggtcg ataatttgtg
    61021 acataaaaca ctcctttaag acagttttga ctggctgtga taaaggttaa accttccagc
    61081 aaggggaagg tcaagaaatt aataaaccag gcgagtaaaa gtccgtaaag attaaaaaat
    61141 tggctcaatt tgcatcagga ttagacattt atctctttgt tttcctgtag ttaagttgcg
    61201 ggtgctaagt taaatcagga tgcctcaaaa tcggcaccgg ggtgaggaat tacctcccgc
    61261 atctataaaa aggagttaac aaaagatgtt agatgcaaac aaattacagc aggcagtgga
    61321 tcaggcttac acccaatttc actcacttaa cggcggtcaa aatgccgact acattccctt
    61381 tctggcgaat gtgcctggtc aactggcggc agtggctatc gtgacctgcg atggcaaggt
    61441 ctatagtgcg ggtgacagtg attaccgctt tgcgctggaa tccatctcga aagtctgtac
    61501 gttagccctt gcgttagagg atgtcggccc gcaggcggta caggacaaaa ttggcgctga
    61561 cccgaccgga ttgcccttta actcagttat cgccttagag ttgcatggcg gcaaaccgct
    61621 ttcgccactg gtaaatgctg gcgccattgc caccaccagc ctgattaacg ctgaaaatgt
    61681 tgaacaacgc tggcagcgaa ttttacatat ccaacagcaa ctggctggtg agcaggtagc
    61741 gctctctgac gaagtcaacc agtcggaaca aacaaccaac ttccataacc gggccattgc
    61801 ctggctgctg tactccgccg gatatctcta ttgtgatgca atggaagcct gtgacgtgta
    61861 cacccgtcag tgctccacgc tcctcaatac tgttgaactg gcaacacttg gcgcgacgct
    61921 ggcggcgggg ggagtgaatc cgctgacgca taaacgcgtt cttcaggccg ataacgtgcc
    61981 gtacattctg gccgaaatga tgatggaagg gctgtatggt cgctccggtg agtgggcata
    62041 tcgcgttggt ttaccgggca aaagcggtgt aggtggcggt attctggcgg tcgtccctgg
    62101 tgtgatggga attgccgcgt tctcaccacc gctggacgaa gaaggcaaca gtgttcgcgg
    62161 tcaaaaaatg gtggcatcgg tcgctaagca actcggctat aacgtgttta agggctgatc
    62221 atgatgaaca cggaaggtaa taacggtaac aaacccctcg gtctatggaa tgtcgtttcc
    62281 atcggtattg gggcaatggt gggggcgggg atcttcgcgc tgctggggca ggctgcgttg
    62341 ctaatggaag cctcgacctg ggtcgccttt gcttttggcg gtattgtggc gatgttttcc
    62401 ggttatgcct atgcgcgtct gggggcgagc tatcccagca atggcggcat tatcgacttc
    62461 tttcgtcgcg gattaggcaa cggcgtcttt tcgctggcgc tctcgttact gtacctgttg
    62521 acgctggcgg tgagcatcgc catggtcgcc cgtgcttttg gcgcttatgc cgtgcagttt
    62581 ttgcatgaag gcagccagga ggagcacctt attttgctct acgcgttggg gatcattgcg
    62641 gtgatgacgc ttttcaactc cttaagcaac catgcggtag ggcggctgga agtgatcctc
    62701 gtcggcatta aaatgatgat cctgttattg ctgattattg ccggtgtctg gtcgctgcaa
    62761 ccggcgcata tttccgtctc tgcgcccccc agctccggtg cgttcttctc ctgtatcggg
    62821 ataaccttcc ttgcctatgc gggctttggc atgatggcga acgcggcgga taaagtgaaa
    62881 gatccgcagg tcattatgcc gcgggcgttt ctggtggcga ttggcgttac cacgttgctt
    62941 tatatctcgc tggcactggt tttgcttagc gatgtatcgg cattagagtt agaaaaatat
    63001 gccgataccg ccgtagcgca ggctgcttct ccgctgctcg gacatgtggg ttatgtgatc
    63061 gtcgtcatcg gcgctttact ggcgacggct tcagccatta acgcgaacct gttcgccgtg
    63121 tttaacatca tggacaacat gggcagcgaa cgcgaactgc cgaagctaat gaataaatcc
    63181 ctgtggcggc agagtacctg gggcaacatc attgtcgtgg tgttgattat gctgatgacg
    63241 gcggcgctaa atttaggctc actcgccagt gttgccagcg ccaccttttt gatttgctac
    63301 ctggcggtgt ttgtggtggc gatccgcctg cgtcatgata ttcacgcctc gttgccgatt
    63361 cttatcgttg gtacgttggt gatgttgttg gtgatcgttg gctttatcta cagtctgtgg
    63421 tcccagggta gccgtgcgtt gatatggatt attggctcac tcttactcag ccttattgtg
    63481 gcaatggtca tgaagcgcaa taaaaccgta taacatctct ctgtgcgcag tacttcctgt
    63541 attattgtgg tggcggtcga tattcgcact ggcaaaaaaa cgtgcttgaa tatctgttga
    63601 aaccctttaa caaagcacag gaggcgttgc gcgaacgatg aacatcagcg atgtagcaaa
    63661 aattaccggc ctgaccagca aagccattcg cttctatgaa gagaaggggc tggtgacgcc
    63721 gccgatgcgc agcgaaaacg gttatcgcac ctacacgcag cagcatctca acgaactgac
    63781 cttactgcgc caggcacggc aggtgggctt taacctggaa gagagcggcg agctggtgaa
    63841 tctgtttaac gacccgcagc ggcacagcgc cgacgtcaaa cggcgcacgc tggagaaggt
    63901 ggcggagatc gaacgacaca ttgaggagct gcaatccatg cgcgaccagc tgctggcact
    63961 ggcgaatgcc tgccctggcg atgacagcgc cgactgcccg attatcgaaa atctctccgg
    64021 ctgctgtcat catcgggcag ggtgattaag acgagacggc tcggatatgt agggttatcc
    64081 cttctatcgc aatgacttca acatgcgtac ctgcaccgag atcctcgctg gcgctgacag
    64141 gccatgaact gtcaccgacg cgcatatgac cgcgcccgtt gaccagcgga gattccagca
    64201 caaaacgtcg gccaatcagc tgctgcccgc gctggtttaa atgactatcg ctgtgctttt
    64261 gttcgcgcac ccgccgcgac aaccatttcc accacagcca ggcggcgagc agcgtcagga
    64321 tggcaaacat taccccttgc cactcccaac ccagcggcac cagccagacc accaggccgg
    64381 taatcaccgc tgccacgccg ctccacaaca aataaccatt tccgcccagc atctcggctg
    64441 ccagcagcaa accgccgaga ctgagccaga aaatatgtgg atgaacgacc attaactcca
    64501 tcatggctga gtccgtttgt tggcgctgtc tttcaccagc tcggcaatcc cggcaatcga
    64561 ccccatcagg ctgctggcct ctaatggcat catcactact ttgctgttac tggaggaacc
    64621 gatctgctgt aacgcttcgg tgtatttctg cgctacgaag tagttcaccg cctgaatatc
    64681 accggaggcg atggcttcag acaccatttt ggtggcgcgg gcttctgctt cagcggaacg
    64741 ttcacgcgct tcagcctgta aaaacgccga ctgacgttcg ccttccgctt tcaggatttg
    64801 cgactgtttt tcaccttcgg ctttgaggat ttccgcctga cggatccctt ccgcttcaag
    64861 aatgtaagcg cgtttggtac gttccgcttt catctgcgcg ttcattgaag agataagctc
    64921 tgccggtggg cgcacgtcgc gaatttcaat acgggtgact ttaatccccc acgggttggt
    64981 ggcttcatcg acaatacgca gcaggcgtga gttgatgctg tcgcgctgag agagcatttc
    65041 gtcaagttcc attgaaccca acacggtacg gatgttagtc atggtcaggt tgatgatcgc
    65101 cagctccaga ttgctgactt cataagccgc gcgcggcgcg tcaatcacct gaataaaaca
    65161 gactgcgtcg atggtaacgt tggcgttatc tttcgagata acttcctggg aagggatatc
    65221 gagcacttgc tccatcatat tgatcttgcg accaatgcga tccataaacg gcaccaccag
    65281 actgagcccc ggctgtaacg ttttggtata gcgaccaaaa cgttctaccg tccattgata
    65341 gccctgcggc acgattttga cgcccgcgcc gacaatgacc agcgcgacaa aaatgagaat
    65401 cgggataaag ataagcatcg gaaaaacctc ctgttgtacc gtccataatc agcaaaattg
    65461 ctgcttgatt aaacaaatta tacctgatta ctgaaagaga gttccccctt attcctgcga
    65521 aggataaact gtttttagta aaaatcagaa aaagggaaca gtgatgcagg aaaatagtcc
    65581 tttgcttcag ctacaaaacg taggatatct ggcgggtgat gcgaagattc ttaataacat
    65641 caatttttcg ctgcgtgctg gcgaatttaa gttaattacc ggtccttccg gttgtggcaa
    65701 aagtacgctg ctaaaaatag ttgcttcatt gatcagccca accagcggaa cgttactgtt
    65761 tgaaggtgag gatgtcagca cactaaagcc agaaatctac cgccagcaag tctcttactg
    65821 cgcccagacg ccgacgctgt ttggcgatac ggtatacgat aatctgatct ttccctggca
    65881 gatccgtaac cagcagcctg acccggccat ttttctcgat tttctcgaac gcttcgcttt
    65941 gccggacagc attttgacga aaaatatcgc cgagctatct ggtggtgaaa aacaacgcat
    66001 ctcactgatt cgtaacctgc aatttatgcc gaaggtttta ttgctggatg aaatcaccag
    66061 tgcgctggat gaaagtaata aacataacgt taatgagatg atccatcgtt atgtgcgcga
    66121 gcaaaatatt gccgtgctgt gggtgacaca cgataaagac gaaattaatc atgcggataa
    66181 agtgattaca ctgcaaccgc atgccggaga aatgcaggaa gcacgctatg aactcgcata
    66241 atattactaa cgaatcattg gcactggcat taatgctggt ggtggtggca atcttaatta
    66301 gccataaaga aaaactggcg ctggagaaag atattttctg gagcgtcggg cgagcgataa
    66361 ttcagctgat tattgtcggc tatgtgctga agtatatttt cagcgtggat gatgccagcc
    66421 tgacattatt gatggtgtta tttatctgct ttaatgcggc gtggaacgcg caaaaacgca
    66481 gtaaatatat tgctaaagct tttatctcat cgtttattgc cattacggtc ggggcgggaa
    66541 ttaccctggc ggtgctgatt ctctctgggt cgattgaatt tatcccgatg caggtgatcc
    66601 ctatcgccgg gatgattgcc ggtaacgcca tggtagcggt ggggttgtgt tacaacaatt
    66661 tagggcaacg ggtcattagc gaacagcaac agattcagga gaaactgagt cttggtgcga
    66721 cgccgaagca ggcttcagcg atattgattc gcgacagtat tcgcgcggct ttaattccga
    66781 cggtcgattc agcaaaaacg gttggcttag tgagtttacc aggaatgatg tccgggctga
    66841 tatttgccgg gattgatccg gtgaaggcga ttaaatatca gattatggtg acctttatgc
    66901 tgctctcaac cgccagcttg tcgaccatta ttgcctgcta tttaacctat cgtaagtttt
    66961 ataattcgcg ccaccagttg gtggtgacgc aattgaagaa gaaatgatgc gatgccggat
    67021 gcgcagcatc gcatccggcg ttgtggttca tgtgccgatc aacggaatat caatacaaca
    67081 atgcatacag ctggcggcga tacttcgacg ccagtgcatc acccgtaccc agcgcagcga
    67141 ggatctcctg gaacgtttta cgcgtctgac cgtctgcggc ggtgagatct ttacgcagat
    67201 gcccgaacag caactccagc gcctcttcat tgcgcccaac ctggtgcagt tgcagagcca
    67261 gttgcgtcgc cagtgcggca tcttctggat tctccgccac ctgctgttgc aactgttgaa
    67321 tttccggcgt atcagccgcc tgcttcagca gttcgatttg cgccaccagc ccctggtagc
    67381 gggtgtcctg atcctgcaac ggaatggttt tcagcaccgc ttccgcatct tcagaacggt
    67441 tcagcgcaat cagcgtttct gccagcagca ggccgatctc cccgttctga ttcgacaact
    67501 gccaggcgtc tttcagcaat ggcagggcat cggtgtaatt gccttcctgc atcagttgca
    67561 tcgcctgctg cgctttcagc tcttcttcgc gcggcagcac tttatccagc agggcgcgga
    67621 tcgcctcttc cggttgcggc ccctggaagc catctaccgg ttgcccgttc tggaacagat
    67681 acacggtcgg aatcgcgcgc agaccaaact gggcggcaat catctgctcc gcgtcgcagt
    67741 ccagcttcgc cagaataaat tgcccgttgt actgcgccgc aaggctttcc agaattgggg
    67801 ttaactgcaa acagtgctgg ctacgttcag accaaaaata gaacagcacc ggagtggtca
    67861 tcgactgttc aagaacctgt tgcaggttag attcgttaat gttgacaata ttttctacgg
    67921 acatggagtc gctctctgtt gtcgatattt tctttgacat gggggcttaa gcgcgcgctt
    67981 caactcaccc ctgcaatatt ttgtccatca cgcgccccgg cagcaggcgc ttaagcacca
    68041 ttactgccca ggtcaccagc gttaccggat agcgcatctt cggcttctcg ctaataaaag
    68101 catggcgcac tttgtccacc accgcttccg gtcccaacgt aaagcgggcg gcgatgccgg
    68161 gattttcgac tggtttatca ctttgcgtct ggttgacgtt gtcggtgaag cgggtacgaa
    68221 taggaccggg ttcgatcagg ctaactttaa ttccgctgtg gcgcagctcc atgcgcagtg
    68281 cgtctgacca cgcctccagc gcatatttac tggccgcgta agcgccgcga cccggcgtgg
    68341 agattaatcc catcaccgat gatgtcatca caatacgccc ttcaccgtgc ggtaacatcg
    68401 cgggtaacag gcgtatggtg agctggtgtg cgccgaaaaa gttggcggaa aactgctgtt
    68461 ccatctgcgc gcggctgatg gtggaaaggg ggccatacat gccgaatccg gcattgttaa
    68521 agatcccata cagacaatta tcggtcaggg cgatcacttc gtcggctgcg cgatcaacac
    68581 tttctggcga atccagatcg atcaacacgc cggtaaatcc catgctgttc atgcgctcaa
    68641 catcatccgg tttccggcaa cctgccagca catgaaaacc ctggcgtttt aattcgagcg
    68701 cgctttccag gccaattcca ctggaacatc cggtaattaa gaccgatttt tgcataactt
    68761 tacctgtcag gatctccgtt gctttatgag tcatgattta ctaaaggctg caactgcttc
    68821 gccatccagt cggcaataaa cggctgggcg tcgcggttgg gatgaatacc gtcatcctgc
    68881 atccattgtg gcttgaggta gacctcttcc ataaaaaagg gcagcagcgg aacatcaaac
    68941 tctttggcga gtttggggta aatggcgcta aaggcttcat tataacggcg accatagttt
    69001 gcaggcagac gtatttgcat taacaatggt tcagcgttgg cggctttgac atcctgcaaa
    69061 atctggcgca gcgtttgctc ggtttgctgt ggctgaaaac cacgcaaacc gtcattgccg
    69121 cccagttcaa ccagcaccca acgcggctga tgctgtttca gcagagccgg aaggcgcgcc
    69181 agtccttgtt gcgaggtgtc gccgctgatg ctggcattaa ctaccgacgt tttactctgc
    69241 cacttatcat tcaacaaggc aggccaggcc gcgctggcag acattcgata cccggcgctc
    69301 aggctatcac ccagaatcaa taacgtgtcc gctgcggcgg cacggaaggt taacaggacc
    69361 aggaacagga agggcaaatg ccagcggaaa acattgttga agttcatcat cttaagaagt
    69421 ccgtcggtca gggggagcat gaactctcca tcctcaccgg agttgagctg gttgtcaaac
    69481 gtggcgagac cattgcactg gtgggcgagt cgggatcggg taagtcaacc ttgctggcga
    69541 tcctcgccgg gctggatgac ggcagcagtg gcgaagtgag tctggtggga caaccgctac
    69601 ataatatgga cgaagaagcg cgggcaaagt tgcgcgcgaa gcacgtaggc tttgtttttc
    69661 agtcatttat gttaattcct acccttaacg ccctggaaaa cgtcgagctt ccggcgctgc
    69721 tgcgcggtga gagtagcgcg gaaagtcgta acggggcgaa agcgttgctc gaacagttag
    69781 ggctgggtaa acgtcttgat catcttccga cacagctttc cggcggcgaa cagcaacgag
    69841 tggcgctggc gcgagccttt aatggtcgac ctgatgtgct gtttgccgac gaacccaccg
    69901 gcaaccttga ccgccagacg ggcgataaaa ttgccgactt gctgttttcc ctcaaccgtg
    69961 agcatggcac cacgttgatt atggtgaccc acgacctgca actggcggca cgctgcgacc
    70021 gctgcttacg gctggtgaac gggcagttgc aggaggaagc atgattgcac gttggttctg
    70081 gcgcgaatgg cgctcgccgt cgctattaat tgtctggctg gcgctaagcc tggcggtggc
    70141 ctgcgtgctg gcgctgggca atatcagcga tcgcatggag aagggcttaa gccagcaaag
    70201 ccgggagttt atggcgggcg atcgggcgtt gcgcagttcg cgcgaagtgc cgcaagcgtg
    70261 gctggaggaa gcgcaaaagc gcggcctgaa agtcggcaag cagctgactt tcgccaccat
    70321 gacatttgca ggcgacacac cgcagctggc gaacgtcaaa gcggtggatg atatctatcc
    70381 gatgtatggc gatctgcaaa ccaatccccc tggcctgaaa ccgcaggcgg gcagcgtatt
    70441 gctggcccca cgcctgatgg cactacttaa cctgaaaacg ggcgacacca ttgacgtggg
    70501 cgatgccacc ttgcggattg ccggagaagt gattcaggaa ccggattccg gttttaaccc
    70561 cttccagatg gctccgcgtc tgatgatgaa tctggcggat gtcgataaaa ccggagccgt
    70621 gcagccgggg agtcgggtca cctggcgtta taaattcggc ggcaacgaga accagctcga
    70681 cggctatgag aaatggttgt taccccagct taaacccgaa caacgctggt acggtctgga
    70741 acaggacgaa ggcgcgctgg ggcgatcgat ggaacgctcg caacagttcc tgctgctttc
    70801 ggcgcttctg accttgctgc tggcagtggc agcggtggcg gtagcgatga atcattactg
    70861 tcgcagtcgc tacgatctgg tggcgatcct caaaacgctg ggggcagggc gagcgcaact
    70921 gcgtaagcta atcgtcggtc agtggttgat ggtgctgacg ctttcagccg ttaccggtgg
    70981 gaccataggc ctgttgttcg aaaacgtgtt gatggtgctg ctcaagcccg ttctgcctgc
    71041 tgcactaccg ccagccagcc tctggccgtg gctgtgggcg cttggcacca tgacggtcat
    71101 ctcgctgctg gtggggctac gaccataccg cttgttgctg gcaacgcagc ctttacgcgt
    71161 attacgtaat gatgtggtag cgaacgtctg gccgctgaag ttttatctgc cgattgtcag
    71221 tgtggtggtt gtgctgctgc tcgccggatt aatgggtggc agcatgctgc tttgggcggt
    71281 gctggcgggc gcggtagtac tggctttgct gtgcggtgtg ctgggctgga tgcagctgaa
    71341 tgtacttcgc cgcatgacgc tgaaatcgct acctctgcgc ctggcggtta gccgcctgtt
    71401 acgtcagccg tggtcaacgt taagccagct ttcggcattt tcgctctcct ttatgctgct
    71461 ggcgctgttg ctggtattgc gtggcgatct gctcgaccgc tggcagcagc agctaccgcc
    71521 agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac cgctaaaagc
    71581 gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc gggcgcggct
    71641 gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta atcgcgaact
    71701 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    71761 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    71821 cggtgatacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    71881 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc ctgaaggggc
    71941 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    72001 gttgacacaa ctcaaccgcc agttcccgac cattagcctg ttagatattg gcgcgatttt
    72061 aaaacaggtc ggtcaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    72121 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg
    72181 tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac
    72241 attgtggtgt gagttcgcca tgcttgggtt tgtttccggc ctggtggccg caattggtgc
    72301 ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat ttcccgtggg agccagactg
    72361 gcgattgtgg attgtgctgc cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg
    72421 gctgggcgcg agactggtta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaca
    72481 ggcgttagat tgcctgatgc gctacgctta tctggtctac gaagatcgtg caatatattg
    72541 attttgcaag attgtgtagg ccggataagg cgtttacgcc gcatccggca tgaacagtgt
    72601 gcattttggc aataaatatg aaaccaactg tcatgtcggt ttttttaaag gccgaaggtg
    72661 gtaaaccttc ggccttagct acgtttcctc agttattcaa taaactgacg gatcagttcc
    72721 agcagcgcga ttagcgcctt aagaacgatg agaactttat cgagaaaagt tttcatccgt
    72781 tgctccctta agggagaaag acaatcacag cttaccctta cgttgcctgt aactatgatt
    72841 ttcttcatgt gctagatgag gactgcctct ctccaaatta gagcaccaga atcagcacca
    72901 cctgtatcct gaccaggact taaaagtact tttccgcact gcggtaatcc cttgaggcag
    72961 tatcactact agcgtcggga ttgtatcgcc aacatagctg tttgaatatg taggcatcct
    73021 gacttataat tcgcttgcta gatgaggact gtttcatttc tgcccggaac caccaagtct
    73081 tgggcggaaa aaataaaaaa accgactgtc atgtcggttt ttttatatct gtattctggt
    73141 gcaaccattc tgttctcacg acaatcgccg tttcagaata ccgcccatca ctgcgcctta
    73201 acccattccg ccacttccgc ccactcaccg cgaaagacaa ctttttccgc ttttttctca
    73261 agctgatagc gatacattgg gtcgtaatat tctttaagta acggcaccag ccaggccaga
    73321 tgaccgtcgg tgctgccggt ggtgagttgc gttgtcagcg ccgtatccag ctgggcagcg
    73381 agttcgttat agcgctgtaa ccccagccga cgcttaatcg ccgaaagtcc gtgatgcagg
    73441 tattcgcaat attcctgcca gccctgttcg tcgccataag cgtgggtaaa atcatgatgc
    73501 atacgcaaga aatactcttc gttcaggcgt tcaagacgga tctcaaacgg atcttctacc
    73561 accgcaatcg ccgcctgagt cattcgctcg cgcagacatt ccggcaggtg attcgaaccg
    73621 atcatccggc tttcgtcttc cagcacccac aggcgcaaat cctgacgggc gtcggttttc
    73681 agcatttcgg cagccagcag gttttcaaaa ctcgcctggc taagttgtgg ttgtaacgtg
    73741 cgaccaaacg ccgaaccgcg atggcgcgcc agcccttcca gatcaacacc gttcggctgt
    73801 tgctgcacta acagcgtttt accgctgcca gtacaaccgc caatcagcac tatcggtttt
    73861 tgtgccagtt caatagtcgc ctgaatcgcg gtctggcgca gtgccttata accgccttcc
    73921 accagcggat aatcaatccc cgctgcatac aaccagcttt gcacaatatg tgagcgctga
    73981 ccgccacggg cgcagcagag aataccttgc ggattttgca ggcacgctgc ccgccaggcg
    74041 tccatgcgct gctgacgaat ttcacccgcc accagtttat gccccagcgc cagcgctgcg
    74101 tctgagcctt gctgtttata gcaggtgcca acggcggcgc gttcatcgtt attcattaac
    74161 ggcagattga tagcggcggg cattgcgcct tgctcaaact cgataggggc gcgaacatca
    74221 ataatgggcg tatcagcaat cagcagggca cgatagtcct gttccgtgtg tctctcttgc
    74281 atagttaaaa gtgaacctca aatcagcttg cgcgctattt tacgcgccaa cgcgcaagga
    74341 aacttgattt ttaactgcgt gggttgccga aaatttctaa aaatccgctg atttccggcc
    74401 tgcgctgggt aaacaaggtc acaatatctt ctaccgcttt gccgctgcca aatttgcgcc
    74461 atgccagact caatggcgaa ggagggcgca tcgttgggat tacccggcta accagttgtt
    74521 gattatcgat cattgactgg caaagcgatt ttggcaaaaa accaatgcca acgcccgcaa
    74581 gatgggcggc gattttcgtt tccatatcag gaacaataat ctctttttgc cctggcaatc
    74641 gccaggcgac gcgtttggtt aaggtgcggg cgctgtcttc aatattgacc gccggaaagc
    74701 gccgcaactg cgcttctgtt agcggctctt caacgttcgc cagcggatga tccgccgcca
    74761 tgacaaagcg ccattgcacc gatcctaagg gatcaagact aaaggtattt gccagcgcct
    74821 cggttcccgt gacgccgata gccagtgaaa aaccttcgta caatagcgaa tcccagacgc
    74881 ccatatagat ttgtcgggag atgtgaaact gggtaaaggg gtaacgttca ttcagccacg
    74941 ccagcaactg ggcgacggcc tgggggttgt agagcaggtt gttgatgaca atattcacct
    75001 ggcgttccac gccatcattc acctgttgca gctcgcttgg catactttcc agccagctca
    75061 gccagtctct ggcctgggaa agtagatgct cgccagccgc tgtcaacgtc acgcttcgag
    75121 tcgtacggaa aaacagcgct actccggtat tctcttccag aagtttaatg cgataactga
    75181 tcgtcgccgt ggttttacat aatcgttctg ccgcttttga aaaacttcct gtttcagcaa
    75241 ccgcaatgaa agtccgcaag gtttctggat cgaacatctt caggtatccc cttttaaatc
    75301 cgcaagttgc gtgattttct tatcctctga tttatcagta tttttacatg ataaccctgt
    75361 tcaatttgtg gactaaatct agttttggaa aaatattcca acttttgtat tgatgttgtt
    75421 ctcttaaggt tttagattgc ctgttattga aaccaagctg accggtcggc gatggttgaa
    75481 cggaattatg ttacaaggac aaaaagatga aacttcaggt attaccgtta agtcaggaag
    75541 cctttagtgc
//
LOCUS       DAFATA010000011        69174 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFATA010000011 DAFATA010000000
VERSION     DAFATA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.011"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1217
                     /gene="wcaL"
                     /locus_tag="LGJ20_001059"
     CDS             <1..1217
                     /gene="wcaL"
                     /locus_tag="LGJ20_001059"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBK2385167.1"
                     /translation="VGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQNTH
                     AAWTKYNLAARTRWLQDEPTGKVAKLRHRASHTLRGIHRKNTWQALNLKRYGAESRNL
                     ILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREVLN
                     HYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPA
                     TPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLE
                     DVVEMPGFKPSHEVKAMLDNADVFLLPSVTSADGDMEGIPVALMEAMAVGIPVVSTLH
                     SGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQV
                     INRELASLLQAL"
     gene            1228..2622
                     /gene="wcaM"
                     /locus_tag="LGJ20_001060"
     CDS             1228..2622
                     /gene="wcaM"
                     /locus_tag="LGJ20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBK2385168.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQMVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            2797..3690
                     /gene="galF"
                     /locus_tag="LGJ20_001061"
     CDS             2797..3690
                     /gene="galF"
                     /locus_tag="LGJ20_001061"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBK2385169.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            3998..5041
                     /gene="wzy"
                     /locus_tag="LGJ20_001062"
     CDS             3998..5041
                     /gene="wzy"
                     /locus_tag="LGJ20_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O126 family O-antigen polymerase"
                     /protein_id="HBK2385170.1"
                     /translation="MYIKFYGFSLMENITIYDYVIVNNGLFNKDAVIVFFIIFYLFGC
                     LINIVSNYVSCIYVLLVLIPLSLLFALNGPDKASYLSLYNAIDTVSDVFKYYNTIDSP
                     LYYITMVLFKNIGLTFYSFMVVQNLIISFSLSYLIYKYKWGYHIAMWLYFTHLLVPFS
                     VRGHLVIITILFANRSVASKIISSLGLVAIHPLISALPITYQLRGLIRGKRSILIIVL
                     TICALVFSSSYLISKYTSYAINGDEYVKNTGLSLIYIVDAIELLLFWSIIRPIALIDK
                     LFLLFPVVLSFGTIIIPMLGRFSYACDLVYILILINNANRLKNKKTLFMCLSLIALIR
                     FVKNYYSIEYFIL"
     gene            5038..6039
                     /locus_tag="LGJ20_001063"
     CDS             5038..6039
                     /locus_tag="LGJ20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 1"
                     /protein_id="HBK2385171.1"
                     /translation="MMLFFKNPKNKKSTGILYLQTLLHERLSNVRVITSTIMIIKRYF
                     NIMKSTEVAIFLPFTFSCIFAKKNDLVIMPDDSIRSITSVSKVRYENKKILKWLFQNI
                     RLIITKNIISLKKDVVYLTVSKDDQWSLQSKFPTHKIVYIPHPIQSASNKEDVLDRCE
                     NISTICFMNLQEHYSVNPSAFIRPKTKVNLNDLHNKVNIIFHGSYSQAWYGKYMYYYG
                     KANCKAVRYVEDFEQFYNNIDLIILPLAAGAGIKNIVLNSVYRNKLILGTEEAFSGIP
                     KEFTRKLIIDSNDDILTKINSEVECLAIKESCNILRDYLVVEHTEKAFMDTIFKNII"
     gene            6049..6942
                     /locus_tag="LGJ20_001064"
     CDS             6049..6942
                     /locus_tag="LGJ20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HBK2385172.1"
                     /translation="MSIIRLQGGLGNQLFQFSFGYALSKINGTPLYFDISHYAENDDH
                     GGYRLNNLQIPEEYLQYYTPKINNIYKFLVRGSRLYPEIFLFLGFCNEFHAYGYDFEY
                     IAQKWKSKKYIGYWQSEHFFHKHILDLKEFFIPKNVSEQANLLAAKILESQSSLSIHI
                     RRGDYIKNKTATLTHGVCSLEYYKKALNKIRDLAMIRDVFIFSDDIFWCKENIETLLS
                     KKYNIYYSEDLSQEEDLWLMSLANHHIIANSSFSWWGAYLGTSASQIVIYPTPWYDIT
                     PKNTYIPIVNHWINVDKHSSC"
     gene            6967..7719
                     /locus_tag="LGJ20_001065"
     CDS             6967..7719
                     /locus_tag="LGJ20_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK2385173.1"
                     /translation="MKRPYFSFLLCVNKDEGFLNDALESIINQDYSYEYEIIVIANNC
                     DDNLWAYLNSVESDKLKIHRTNIGQLAFNLNYGANIARGEYIVRMDADDVSTPNRLKI
                     LEAAIQENNYPDVIGTSVYFISETGRIIKKYVPPLGEVKSALIKNPFVHPTVAIKKVS
                     LFKIGGYLGGFQSEDNDLWIRMLRAHEFKLVNIDSVTLFYRINDHQTRGRVLPYAEVA
                     SYALREFLLKKRIKYFAALLINIGKALILPKR"
     gene            7732..8853
                     /locus_tag="LGJ20_001066"
     CDS             7732..8853
                     /locus_tag="LGJ20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK2385174.1"
                     /translation="MKIMHTVLNTFTAKKFVEPLLDNVNEQHNKEKIIHKILIDPVNG
                     DERFLKELHVHYEIKKFDIDYLFIKHIIELYFYFRKERPDIVIAHMSRGAILPLISAF
                     IARVPKRIYFNHGVPYIGYKNLPRMCFKLLEKLNCFFSNQVLTVSQGMIEPLTAITKK
                     PVSILGPGSACGIDHNEFQRKSKYDIENIKQQYAASDTFIFTFVGRPVARKGFDITLK
                     AFTKAFSGADNVILLLAGATSEDIFRVLGSKPNNIKPLGFVMDLDNVYLATDVVVVPS
                     EHEGFCYALLEGAARECALICSDIPGPDAIVINKRNGILITPKNIDSLMVSMSELFND
                     REMTKKYGEQAYMIASEFERKKIMLYYSYYIRSLFIEQR"
     gene            8926..10047
                     /gene="gmd"
                     /locus_tag="LGJ20_001067"
     CDS             8926..10047
                     /gene="gmd"
                     /locus_tag="LGJ20_001067"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBK2385175.1"
                     /translation="MKKKALITGITGQDGSYLAEFLLDKGYEVHGIKRRASLFNTNRI
                     DHIYQDLHHEKQSFFLHYGDLTDSLNIVRLVQEIQPDEIYNLGAQSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRICGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KMYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRALANIAYGLEPCLYL
                     GNMDALRDWGHAKDYVRMQWLMLQQETPDDFVIATGKQISVREFVRMAAANLGIELEF
                     IGCGLKEVGVVKAIHGNEAPSVKIGDVIVQVDPRYFRPAEVETLLGYPEKAKIKLGWE
                     PEITIEEMCKEMIRNDLNIAKQHSLLKLNGYDVSLSVEK"
     gene            10052..11017
                     /locus_tag="LGJ20_001068"
     CDS             10052..11017
                     /locus_tag="LGJ20_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBK2385176.1"
                     /translation="MKMKKIYIAGHRGMVGSAIKRQLMKNSSVELIFRSSHELDLMNQ
                     DAVQKFFNTERIDEVYLAAAKVGGIYANNKYPADFIYNNILIQANVINSAHRAGVQKL
                     LFLGSSCIYPKFAEQPIKEESLLCGSLEPTNEPYAIAKITGIKLCESYNRQYGRDYRS
                     VMPTNLYGPNDNFHHENSHVLPALIRRFHEAKINNLPEVSVWGSGNALREFLHVDDMA
                     AASIYIMNLPNERYKEYTKPECSHINVGSGKDYSIRQLAEMVAEITNYKGNIYFDSTK
                     PDGTPRKLMSNERLSKLGWSPKIELYDGVKDTYQWFLNNIENIRK"
     gene            11104..12495
                     /locus_tag="LGJ20_001069"
     CDS             11104..12495
                     /locus_tag="LGJ20_001069"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HBK2385177.1"
                     /translation="MLFPIIMAGGTGSRLWPLSRESFPKQYLKLYGDMTMLQSTIARL
                     NTLDCSTPLVICNEEHRFIVAEQLKKINKLDKNILLEPQGKNTAPAITLCAFAALKYA
                     KNDEDPLLLVLAADHVIGNISSFTKSVKNALPHAHAGKLVTFGIIPTTPETGYGYIHR
                     GEKFNDAYKVSSFTEKPDIYRAQEFLKTGEYYWNSGMFLFSAKRLLAEMEKYRPDIFS
                     ACEQAFLSPSNDLDFIRVDRDKFSLCPSESIDYAVMENTDDAIVVPMDAEWSDVGSWS
                     SLWDISDKDVNGNAVSGDLISFNSYNNFIKAEDKLVALVGVNDLVLVHTDDAILVSSK
                     YEVQNVKKIVEVLNQNGRDESKKHKEVYRPWGEYHYITEAEKYNVRRVLINPGQKIST
                     QTHNHRSEHWVIVSGIAKITINKDINIIKENESIYIPPGTMHTIENSEMLPLEIIEIQ
                     TGQLLTEDDVIRP"
     gene            12566..14005
                     /locus_tag="LGJ20_001070"
     CDS             12566..14005
                     /locus_tag="LGJ20_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HBK2385178.1"
                     /translation="MSKKFNTLSIIRNSGVAFGTSGARGLVTEFTPEVCGAFSHAFIN
                     VMKQKYKFHGVALAIDNRPSSYSIAQACASVIAQYGLKVNYYGVIPTPALAYKALEDK
                     IPCIMVTGSHIPHDRNGLKFYSPIGEITKEDEIRIKDVSIDFTLDSSIPNLYPCPDAA
                     SCYFKRYIDFFPEGILKGKRVGFYEHSSAGRDLYIPIFEKLGAEIVSIGRSDKFIPID
                     TEAVGHDLVIQAKKWALEYKLDAIFSTDGDGDRPMLSDENGKWLRGDILGLLCAETLN
                     IKSIAIPISCNSLIMQRKCFEEVVLTKIGSPYVLDAFSSLKDKYQNYAGFEANGGFIL
                     GSDITVDNRKIKALPTRDALLPILVLLSANKKISSLVHSLPSRFTYSDRIANFLPEKR
                     DRLFTICKNNPHTIVDLLGENEAGIEVKDINNLDGYRFTLNNNVIIHLRSSGNAPELR
                     CYCEADSEFCAQKYVERILSSVACLIKIN"
     gene            14109..15593
                     /gene="wzx"
                     /locus_tag="LGJ20_001071"
     CDS             14109..15593
                     /gene="wzx"
                     /locus_tag="LGJ20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O126 family O-antigen flippase"
                     /protein_id="HBK2385179.1"
                     /translation="MQNNKRVVFMNVSLNIMQQLINTVYTFIIPPLIIINYGSDINGL
                     ISSVKQCLVYLALVEAGVGGAAIVALFKPLSEGDRVLQSQIISEAKYFYSKIGVVFII
                     LLLLFSIIYAQIISDQINIILAISLFVIMGLINAVDFLLISKYQVFLTADNKLYVTSA
                     TKILTIILDMGCAYILITEGMNIILVQICSVFIYMIRYVIYYFYFLKKYSYISFDLKN
                     KTALLHQKSAVLIHQISGALVFNSPMILITIFLNLKAASIYSIYMLIFMAIKVFLSTI
                     TNGMQSVFGRMIVNENINAVREYFSKFEILYFSCLGWFYSCALILSYDFIYLYTLKFN
                     DPDYVQTILIYLFVIIGIADNIRVPGMTLIAAKGHYEQTKYRSIIEALLNIVFSIVFI
                     KIYGLPGVLLGSIISYSYRTIDIIYYSSNKILKQSIIFKMIMLFFLVFFILANYFIVE
                     SLKMPFFINDFFKWILYSTFVAVLSALLWLLFYKIMRLMNDKTY"
     gene            15700..17106
                     /gene="gndA"
                     /locus_tag="LGJ20_001072"
     CDS             15700..17106
                     /gene="gndA"
                     /locus_tag="LGJ20_001072"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK2385180.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKAFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
     gene            17355..18521
                     /gene="ugd"
                     /locus_tag="LGJ20_001073"
     CDS             17355..18521
                     /gene="ugd"
                     /locus_tag="LGJ20_001073"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBK2385181.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            18667..19644
                     /gene="wzzB"
                     /locus_tag="LGJ20_001074"
     CDS             18667..19644
                     /gene="wzzB"
                     /locus_tag="LGJ20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBK2385182.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(19740..20351)
                     /locus_tag="LGJ20_001075"
     CDS             complement(19740..20351)
                     /locus_tag="LGJ20_001075"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBK2385183.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(20345..21121)
                     /gene="hisF"
                     /locus_tag="LGJ20_001076"
     CDS             complement(20345..21121)
                     /gene="hisF"
                     /locus_tag="LGJ20_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBK2385184.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(21103..21840)
                     /gene="hisA"
                     /locus_tag="LGJ20_001077"
     CDS             complement(21103..21840)
                     /gene="hisA"
                     /locus_tag="LGJ20_001077"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBK2385185.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(21840..22430)
                     /gene="hisH"
                     /locus_tag="LGJ20_001078"
     CDS             complement(21840..22430)
                     /gene="hisH"
                     /locus_tag="LGJ20_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBK2385186.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGSAGAKLLKNFLEM"
     gene            complement(22430..23497)
                     /gene="hisB"
                     /locus_tag="LGJ20_001079"
     CDS             complement(22430..23497)
                     /gene="hisB"
                     /locus_tag="LGJ20_001079"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBK2385187.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPSVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLTADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(23497..24567)
                     /gene="hisC"
                     /locus_tag="LGJ20_001080"
     CDS             complement(23497..24567)
                     /gene="hisC"
                     /locus_tag="LGJ20_001080"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBK2385188.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALQEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(24564..25868)
                     /gene="hisD"
                     /locus_tag="LGJ20_001081"
     CDS             complement(24564..25868)
                     /gene="hisD"
                     /locus_tag="LGJ20_001081"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBK2385189.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            complement(25874..26773)
                     /gene="hisG"
                     /locus_tag="LGJ20_001082"
     CDS             complement(25874..26773)
                     /gene="hisG"
                     /locus_tag="LGJ20_001082"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBK2385190.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGICFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(26820..26943)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(26919..26969)
                     /gene="hisL"
                     /locus_tag="LGJ20_001083"
     CDS             complement(26919..26969)
                     /gene="hisL"
                     /locus_tag="LGJ20_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBK2385191.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            27252..27503
                     /gene="yefM"
                     /locus_tag="LGJ20_001084"
     CDS             27252..27503
                     /gene="yefM"
                     /locus_tag="LGJ20_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBK2385192.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            27500..27754
                     /gene="yoeB"
                     /locus_tag="LGJ20_001085"
     CDS             27500..27754
                     /gene="yoeB"
                     /locus_tag="LGJ20_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBK2385193.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            27837..28661
                     /locus_tag="LGJ20_001086"
     CDS             27837..28661
                     /locus_tag="LGJ20_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2385194.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEASRMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            28707..29636
                     /locus_tag="LGJ20_001087"
     CDS             28707..29636
                     /locus_tag="LGJ20_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2385195.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            29851..29913
                     /gene="yoeI"
                     /locus_tag="LGJ20_001088"
     CDS             29851..29913
                     /gene="yoeI"
                     /locus_tag="LGJ20_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBK2385196.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            29903..31261
                     /gene="plaP"
                     /locus_tag="LGJ20_001089"
     CDS             29903..31261
                     /gene="plaP"
                     /locus_tag="LGJ20_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBK2385197.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPEASQPEI
                     MLYVAGKTFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            31440..32498
                     /gene="yeeE"
                     /locus_tag="LGJ20_001090"
     CDS             31440..32498
                     /gene="yeeE"
                     /locus_tag="LGJ20_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBK2385198.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHVSGLNQTLQHYSTEHNSIAETFNLSVWPLIAVL
                     LVITFWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            32512..32739
                     /locus_tag="LGJ20_001091"
     CDS             32512..32739
                     /locus_tag="LGJ20_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBK2385199.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(32782..34209)
                     /gene="sbcB"
                     /locus_tag="LGJ20_001092"
     CDS             complement(32782..34209)
                     /gene="sbcB"
                     /locus_tag="LGJ20_001092"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBK2385200.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLNNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            34418..35584
                     /gene="dacD"
                     /locus_tag="LGJ20_001093"
     CDS             34418..35584
                     /gene="dacD"
                     /locus_tag="LGJ20_001093"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBK2385201.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            35703..36176
                     /gene="sbmC"
                     /locus_tag="LGJ20_001094"
     CDS             35703..36176
                     /gene="sbmC"
                     /locus_tag="LGJ20_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBK2385202.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            36375..37433
                     /locus_tag="LGJ20_001095"
     CDS             36375..37433
                     /locus_tag="LGJ20_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK2385203.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            37605..37934
                     /locus_tag="LGJ20_001096"
     CDS             37605..37934
                     /locus_tag="LGJ20_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBK2385204.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(38035..38217)
                     /locus_tag="LGJ20_001097"
     CDS             complement(38035..38217)
                     /locus_tag="LGJ20_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBK2385205.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTNAVNNH
                     GVTELFALLHTEDVCR"
     gene            38271..38417
                     /locus_tag="LGJ20_001098"
                     /pseudo
     CDS             38271..38417
                     /locus_tag="LGJ20_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(38706..38819)
                     /locus_tag="LGJ20_001099"
     CDS             complement(38706..38819)
                     /locus_tag="LGJ20_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBK2385206.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(38832..39026)
                     /locus_tag="LGJ20_001100"
     CDS             complement(38832..39026)
                     /locus_tag="LGJ20_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385207.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            complement(39023..39396)
                     /gene="cbtA"
                     /locus_tag="LGJ20_001101"
                     /pseudo
     CDS             complement(39023..39396)
                     /gene="cbtA"
                     /locus_tag="LGJ20_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            complement(39485..39853)
                     /gene="cbtA"
                     /locus_tag="LGJ20_001102"
     CDS             complement(39485..39853)
                     /gene="cbtA"
                     /locus_tag="LGJ20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HBK2385208.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(39927..40148)
                     /locus_tag="LGJ20_001103"
     CDS             complement(39927..40148)
                     /locus_tag="LGJ20_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBK2385209.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(40217..40693)
                     /locus_tag="LGJ20_001104"
     CDS             complement(40217..40693)
                     /locus_tag="LGJ20_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBK2385210.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(40709..41188)
                     /locus_tag="LGJ20_001105"
     CDS             complement(40709..41188)
                     /locus_tag="LGJ20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBK2385211.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(41270..42091)
                     /locus_tag="LGJ20_001106"
     CDS             complement(41270..42091)
                     /locus_tag="LGJ20_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBK2385212.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(42312..42722)
                     /locus_tag="LGJ20_001107"
     CDS             complement(42312..42722)
                     /locus_tag="LGJ20_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385213.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(42738..43421)
                     /locus_tag="LGJ20_001108"
     CDS             complement(42738..43421)
                     /locus_tag="LGJ20_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385214.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            complement(43557..44627)
                     /locus_tag="LGJ20_001109"
     CDS             complement(43557..44627)
                     /locus_tag="LGJ20_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK2385215.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLCDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(44624..45529)
                     /locus_tag="LGJ20_001110"
     CDS             complement(44624..45529)
                     /locus_tag="LGJ20_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBK2385216.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITQVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(45526..47922)
                     /locus_tag="LGJ20_001111"
     CDS             complement(45526..47922)
                     /locus_tag="LGJ20_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HBK2385217.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            complement(48140..49011)
                     /locus_tag="LGJ20_001112"
                     /pseudo
     CDS             complement(48140..49011)
                     /locus_tag="LGJ20_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            complement(49095..50246)
                     /locus_tag="LGJ20_001113"
     CDS             complement(49095..50246)
                     /locus_tag="LGJ20_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385218.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            complement(51514..51783)
                     /locus_tag="LGJ20_001114"
                     /pseudo
     CDS             complement(51514..51783)
                     /locus_tag="LGJ20_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            51869..52891
                     /gene="istA"
                     /locus_tag="LGJ20_001115"
     CDS             51869..52891
                     /gene="istA"
                     /locus_tag="LGJ20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HBK2385219.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            52891..53670
                     /gene="istB"
                     /locus_tag="LGJ20_001116"
     CDS             52891..53670
                     /gene="istB"
                     /locus_tag="LGJ20_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBK2385220.1"
                     /translation="MMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            53709..53861
                     /locus_tag="LGJ20_001117"
     CDS             53709..53861
                     /locus_tag="LGJ20_001117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385221.1"
                     /translation="MDHFSSVVDICGQSFWLAVQGMFSGYRVPQSRDIAPIVTFQIDR
                     GLKMWH"
     gene            complement(54599..55201)
                     /locus_tag="LGJ20_001118"
     CDS             complement(54599..55201)
                     /locus_tag="LGJ20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385222.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(55295..55501)
                     /locus_tag="LGJ20_001119"
     CDS             complement(55295..55501)
                     /locus_tag="LGJ20_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBK2385223.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            57028..57597
                     /locus_tag="LGJ20_001120"
     CDS             57028..57597
                     /locus_tag="LGJ20_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBK2385224.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            57857..58258
                     /locus_tag="LGJ20_001121"
     CDS             57857..58258
                     /locus_tag="LGJ20_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385225.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            58246..58653
                     /locus_tag="LGJ20_001122"
     CDS             58246..58653
                     /locus_tag="LGJ20_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBK2385226.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPSCGTGVYSREGGQEGNWKDRN"
     gene            58937..60556
                     /locus_tag="LGJ20_001123"
                     /pseudo
     CDS             58937..60556
                     /locus_tag="LGJ20_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            60636..60928
                     /locus_tag="LGJ20_001124"
                     /pseudo
     CDS             60636..60928
                     /locus_tag="LGJ20_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(60968..62002)
                     /locus_tag="LGJ20_001125"
     CDS             complement(60968..62002)
                     /locus_tag="LGJ20_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HBK2385227.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQ
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(62005..62970)
                     /locus_tag="LGJ20_001126"
     CDS             complement(62005..62970)
                     /locus_tag="LGJ20_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2385228.1"
                     /translation="MKKNKYSTPLWVLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKEIRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            complement(63027..63785)
                     /locus_tag="LGJ20_001127"
                     /pseudo
     CDS             complement(63027..63785)
                     /locus_tag="LGJ20_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            complement(63799..65013)
                     /locus_tag="LGJ20_001128"
                     /pseudo
     CDS             complement(63799..65013)
                     /locus_tag="LGJ20_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            complement(65209..65569)
                     /locus_tag="LGJ20_001129"
                     /pseudo
     CDS             complement(65209..65569)
                     /locus_tag="LGJ20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            65631..66321
                     /locus_tag="LGJ20_001130"
                     /pseudo
     CDS             65631..66321
                     /locus_tag="LGJ20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(66509..67009)
                     /locus_tag="LGJ20_001131"
                     /pseudo
     CDS             complement(66509..67009)
                     /locus_tag="LGJ20_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            68681..>69174
                     /gene="cobU"
                     /locus_tag="LGJ20_001132"
     CDS             68681..>69174
                     /gene="cobU"
                     /locus_tag="LGJ20_001132"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBK2385229.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAA"
BASE COUNT        18121 a        15646 c        16640 g        18767 t
ORIGIN      
        1 aggtcggctt ctttttactg aaatttccgc tgtcttcgga aaccttcgtc ctcaatcaaa
       61 ttaccgcgtt tattgatatg ggctttgagg tggagattgt cgcgctgcaa aaaggcgaca
      121 cccagaacac ccacgcggca tggacgaaat acaaccttgc cgccagaacc cgctggttac
      181 aggacgaacc tacgggcaaa gtggcgaagc tacgccaccg tgccagccat acgttacgag
      241 gcattcatcg taaaaatacc tggcaggcgc tcaacctcaa acgctatggc gcagagtcgc
      301 ggaatctgat tttgtctgcc atttgcggcc aggtcgcaac accgtttcgc gccgatgtgt
      361 tcatcgctca ttttggtcca gcgggggtaa ccgcagcaaa actccgcgaa ctgggtgtca
      421 ttcgcggcaa aattgccact atcttccacg gcattgatat ctccagtcgg gaagttctca
      481 accactacac ccccgaatat caacaactgt ttcgccgtgg cgacctgatg ctaccgataa
      541 gcgatctgtg ggccggaagg ctgcaaaaaa tgggctgccc gagggaaaaa atcgccgtat
      601 cgcgcatggg cgtggacatg acgcgtttta gtccgcgtcc ggtgaaagcg cccgcaacac
      661 cgctggaaat catttccgtc gcacgcttaa ccgagaaaaa aggcctgcat gtggcgatcg
      721 aagcctgccg tcagttgaaa gagcagggcg tggcatttcg ctatcgcatc ctcggcattg
      781 gcccgtggga acgacgcctg cgcacgctca tcgaacaata tcaactggaa gatgtggtgg
      841 agatgccggg ctttaaaccg agccacgaag tgaaagcgat gctcgacaac gcggatgttt
      901 tcttgttgcc atcggtaacg agcgcggacg gcgatatgga aggtattccg gtggcgctga
      961 tggaagcgat ggcggtcggc attccggtgg tttctactct gcatagcgga ataccggaat
     1021 tggtggaggc cgataaatcc ggctggctgg tgcctgagaa cgatgctcgc gcactggcgc
     1081 aacgactggc ggcgtttagc caactggaca ccgacgaatt ggctccggtc gtcaaacgcg
     1141 cgcgcgaaaa agttgaacac gattttaacc agcaggtgat caatcgagaa ctcgccagct
     1201 tgttacaggc tttatagagg agaacgtatg ccatttaaaa aactctcccg acgcaccttc
     1261 ctgacggcaa gctcggcgct tgccttcctc catacccctt tcgctcgcgc acttcccgcc
     1321 cgacaaagcg ttaacattaa cgactacaac ccacacgact ggatcgcctc atttaaacaa
     1381 gccttcagcg aagggcaaat ggtcgtcgtg cctgccggat tcgtttgtga caatatcaac
     1441 accggcatct tcattcctcc tggcaaaacg ttacacatcc tcggaagcct gcgaggcaac
     1501 ggcagagggc gatttgtctt acaggacggc agccaggtga caggggagga gggcggcagt
     1561 atgcataaca tcaccctgga tgtgcgtggt tctgactgca ccatcaaagg gctggcgatg
     1621 agcggctttg gtccggtaac gcagatttat atcggcggca aaaacaaacg ggtcatgcgc
     1681 aacctgacca tcgataacct cactgtcagc cacgctaatt acgccatctt acgccaggga
     1741 tttcataacc agattatcgg tgccaacatc accaactgta aatttagtga tttacagggc
     1801 gacgctattg aatggaacgt ggcgattaac gacagtgata ttttgatctc cgaccacgtt
     1861 attgagcgca tcaactgtac caacggcaaa atcaactggg gcatcggcat aggccttgcg
     1921 ggaagtacct acgataacaa ctacccggaa gaccaggcag tgaaaaactt tgtcgtggcg
     1981 aatatcacgg gatcggattg tcggcagttg atccatgttg aaaatggtaa acattttgtt
     2041 attcgtaata tcaaagcccg caatatcacg ccggatttca gtaagaaagc aggtattgat
     2101 aacgccacag tcgctattta cggttgtgac aattttgtga ttgataatat tgaaatgatt
     2161 aatagcgccg gaatgttaat cggctatggg gtaattaaag gcaaatatct ctcgataccg
     2221 caaaatttcc gagtgaataa tattcaactg gataataccc accttgctta taaattgcgc
     2281 ggtatccaaa tctctgccgg gaatgccgtc tcctttgtgg cactgactaa cattgagatg
     2341 aagcgtgcct cgctggagct acacaacaag ccgcaacatc tttttatgcg taatatcaaa
     2401 gtgatgcagg aatcctctgt tggaccagca ttgagcatga acttcgacat gcgcaaagac
     2461 gttcgcggcg tctttatggc gaaaaaagaa acactgctgt ctcttgcaaa tgttcatgcg
     2521 gtgaatgaga aaggacaaag ctccgtcgat atcgacaggg ttaatcacca tattgttaat
     2581 gtggaaaaga ttaactttag attgccggaa cggagagagt agatttgcga ccattcctgg
     2641 aaaaatggag ccatacttag gaacaatgct actgcaatcc acaacgaagc ggcgtaacat
     2701 cacaagtaat tcagtaatca attcagggta attgatgctg gcgaaaaaaa tcgaacaagc
     2761 tataattcag caaccatttt acaggtggat gaaataatga cgaatttaaa agcagttatt
     2821 cctgtagcgg gtcttgggat gcatatgttg cctgccacta aggcgattcc caaagagatg
     2881 ctaccgatcg tcgacaagcc aatgattcag tacattgttg acgagattgt ggctgcaggg
     2941 atcaaagaaa tcctcctggt aactcacgcg tccaagaacg cggtcgaaaa ccacttcgac
     3001 acctcttatg aattagaatc tctccttgaa ctgcgcgtga agcgtcaact gctggcggaa
     3061 gtacagtcca tttgcccgcc gggcgtgaca attatgaacg tgcgtcaggg tgaaccttta
     3121 ggtttgggcc actccatttt atgtgcacga cctgctattg gtgacaatcc atttgtcgtg
     3181 gtgctgccag acgttgtgat cgacgacgcc agcgccgacc cgctgcgcta caaccttgca
     3241 gccatgattg cgcgcttcaa cgaaacggga cgcagccagg tgctggcaaa acgtatgccg
     3301 ggtgacctct ctgaatactc cgtcatccag accaaagagc cgctggatcg tgaaggcaaa
     3361 gtcagccgca ttgttgaatt tatcgaaaaa ccggatcagc cgcagacgct ggactcagac
     3421 atcatggccg tgggccgtta tgtgctttct gccgatattt ggccggaact tgaacgcacg
     3481 cagcctggtg cgtggggacg tattcagctg actgatgcta ttgccgagct ggcgaaaaaa
     3541 cagtccgttg atgccatgct gatgacaggt gacagctacg actgcggtaa aaaaatgggc
     3601 tatatgcagg cgtttgtgaa gtatgggctg cgcaacctga aagaaggggc gaagttccgt
     3661 aaagggattg agaagctgtt aagcgaataa tgaaaatctg accggatgta acggttgata
     3721 agaaaattat aacggcagtg aagattcgtg gcgaaagtaa tttgttgcga attttcctgc
     3781 cgttgtttta tataaacaat cagaataaca acgagttagc aataggattt tagtcaaagt
     3841 tttccaggat tttccttgtt tccagagcgg attggtaaga caattagcgt ctgaattttt
     3901 cgggtttagc gcgagtgggt aacgctcgtc acatcgtaga catgcatgca gtactctggt
     3961 agctgtaaag ccaggggcgg tagcgtgcat aattgaaatg tacatcaaat tttatggttt
     4021 ttctctaatg gaaaatataa ccatttacga ttatgtaatt gtaaataacg gtctatttaa
     4081 taaagatgct gtaatcgttt tttttataat attttatttg tttggttgtc ttattaatat
     4141 tgtatccaat tatgttagct gtatttatgt tctattggta ctaataccat tatcactatt
     4201 attcgcatta aatggacctg ataaagcatc gtatttgtct ttatataatg caatagatac
     4261 agtgagcgat gtttttaaat attacaatac aatagattct ccattatatt atataacaat
     4321 ggtcttattt aaaaatatag ggcttacatt ttacagtttt atggtagtgc aaaatttaat
     4381 tatatcattt tcattatcat atctaattta taaatataaa tgggggtatc acatagcaat
     4441 gtggctatat tttacccatc tactcgtacc attttcggtt cgtggacatt tggtcataat
     4501 taccatttta tttgcaaata ggagtgtggc atctaaaatt attagttcgt taggattagt
     4561 ggcgattcat ccactaatat ctgcattacc aatcacttat caattgcgtg gcttgataag
     4621 aggtaaacgt agtattctaa taatcgtgct aacaatatgt gcgctagtat tttcatcatc
     4681 ctatttgatt agtaagtata ctagttatgc tataaacggt gatgagtatg ttaaaaacac
     4741 ggggttatca ctaatatata tagttgatgc aatagagtta ttgctatttt ggtcaataat
     4801 tagaccaata gctttaatcg ataagttatt tttacttttc ccggtcgtat taagttttgg
     4861 tacaataata atcccaatgc ttggtcgatt ttcatatgca tgtgatttgg tatatatttt
     4921 aattttaatt aataacgcaa ataggctaaa aaataaaaag acacttttta tgtgtttatc
     4981 acttattgca ttaatccgat ttgttaaaaa ttattattca attgagtatt ttatattatg
     5041 atgctttttt ttaaaaaccc caaaaataaa aaatcaactg gtattctata tctccagacg
     5101 ttattacatg aaaggttatc aaatgtaaga gtgataacat caacgataat gattattaaa
     5161 cgttatttca atataatgaa atctacagag gtagcaattt ttcttccctt tactttctct
     5221 tgcatatttg caaaaaaaaa tgatttggta ataatgcctg atgactcaat tcgatcaata
     5281 accagcgttt caaaagtgag gtatgaaaat aaaaaaattt taaaatggct atttcagaat
     5341 atcaggttaa ttataactaa gaatataata tctttaaaaa aagacgttgt ctatttaacc
     5401 gtctctaaag atgatcagtg gtcattgcaa tcaaaattcc caacgcataa aattgtctat
     5461 attcctcatc ctatacaatc tgcaagtaat aaagaagatg tgttggatag atgcgaaaat
     5521 ataagtacta tatgttttat gaatttacag gaacattatt ctgttaatcc aagtgctttt
     5581 attcggccta aaacaaaagt taatctgaat gatttgcata ataaagttaa tattattttt
     5641 catggttctt attctcaagc ctggtacggg aagtatatgt attattatgg gaaagcaaat
     5701 tgcaaagcag taaggtatgt cgaggatttc gaacagttct ataataatat agatctaatc
     5761 attttgccat tagcagccgg tgcgggaatt aagaatatcg ttttaaattc agtatacagg
     5821 aataaactga ttttaggtac cgaagaggca ttttcaggaa tacctaagga atttacaaga
     5881 aaattaatta ttgattcgaa tgatgacatc ttaacaaaaa ttaactcaga agtagagtgt
     5941 ttagccatta aggaaagttg taatatttta cgtgactatc tagtcgttga acacacagaa
     6001 aaagctttta tggatacaat ttttaaaaac ataatttaaa gagcaaatat gtctattata
     6061 agattacaag gcggacttgg aaatcaactt tttcagttct catttgggta tgcgctttcc
     6121 aaaattaatg ggacaccatt atattttgat ataagtcatt atgctgaaaa tgatgatcat
     6181 ggtggttaca ggctaaacaa tctacaaatt ccagaggaat atttacagta ttacacacca
     6241 aaaattaata atatttataa atttttggtt cgtgggtcaa gattatatcc tgaaatcttt
     6301 ctttttttag gtttttgcaa tgaatttcac gcctatggtt atgattttga atatatagcg
     6361 caaaaatgga aatccaaaaa atatataggg tattggcaat ctgaacactt tttccataaa
     6421 catatattag atctaaaaga attttttatt ccaaagaatg tgtctgaaca agcaaattta
     6481 cttgcagcaa aaattcttga atctcaatca tcactttcta ttcatataag aagaggagat
     6541 tatataaaaa acaaaacagc tactttaact catggcgttt gttcgttaga gtattacaaa
     6601 aaagctttaa ataaaatacg cgatttggca atgatacgtg acgtgtttat tttcagtgat
     6661 gatatttttt ggtgtaaaga aaatatcgaa acattactca gtaaaaaata taatatatat
     6721 tattcagaag atttatcaca agaagaagat ttatggttaa tgagcttagc taaccatcat
     6781 attatagcga atagtagttt tagttggtgg ggggcttatt taggtacatc agcgtcacag
     6841 attgttattt atcctactcc ttggtacgat ataactccaa aaaatactta tatccccata
     6901 gtcaatcact ggataaacgt ggataaacat agctcgtgtt agggatagat tataaaggag
     6961 cttgtagtga agagaccata tttttcgttt ttattatgcg ttaacaaaga tgaaggtttt
     7021 ctcaatgatg ctcttgagag tattataaac caagactatt cttatgaata tgaaattatt
     7081 gttattgcaa ataattgtga tgataatcta tgggcatatt tgaactccgt tgagagtgac
     7141 aaattgaaaa tacatcgtac caatattggg cagttagctt ttaatttaaa ctatggagca
     7201 aatattgcta gaggggagta tattgttagg atggatgccg atgatgtatc caccccgaat
     7261 cgattaaaga ttcttgaggc ggctattcaa gaaaataact atccagatgt aataggaact
     7321 tcagtttatt ttataagtga gacaggtaga atcattaaga aatatgttcc tcctttagga
     7381 gaggtaaaaa gtgctctcat taaaaatcca ttcgttcacc caactgtggc catcaaaaaa
     7441 gtgtcattat ttaaaattgg gggctatctt ggtggatttc aatcagaaga taatgattta
     7501 tggattcgga tgcttagagc gcatgaattt aagttagtaa atattgattc tgtaacatta
     7561 ttttatagaa taaacgatca tcaaactcgt ggtcgagttc taccatatgc agaagttgcg
     7621 tcttatgcgt tgagagaatt tcttttaaaa aagagaataa aatattttgc ggcgcttctt
     7681 atcaatattg gaaaggcgct tatacttcca aaaagataag gtatgaaact tatgaaaata
     7741 atgcatacgg tattaaatac atttactgca aagaagtttg ttgagccgct tttagataat
     7801 gttaatgagc aacacaataa agaaaaaata attcataaaa ttttgattga tcctgttaat
     7861 ggtgatgagc gttttttaaa agaacttcat gtacattatg aaataaaaaa atttgatata
     7921 gattatttat ttatcaaaca tataattgag ctttatttct attttagaaa agaaagacca
     7981 gatatagtta tagcccatat gtcaagaggg gctattctac cgttaatttc tgcttttata
     8041 gcccgagtac ccaaacgtat atattttaat catggggtgc catatattgg ttataagaac
     8101 cttcctagga tgtgttttaa attacttgaa aagctaaatt gttttttttc taaccaagta
     8161 ttaactgttt ctcaaggtat gattgagcct ttaaccgcta taactaaaaa accagtatca
     8221 atacttgggc ctggctctgc ttgtggtatt gaccacaatg agtttcaaag aaagtcaaaa
     8281 tatgatatag aaaatataaa acagcaatac gctgcatcag atactttcat ctttaccttc
     8341 gtaggaaggc cggttgcacg aaaagggttt gatataacgc tgaaagcgtt tactaaggca
     8401 tttagtggtg cagataatgt aattctttta ttagcagggg caacttcgga agatattttt
     8461 cgcgtattag gaagtaaacc taataatatc aaaccattag gttttgtaat ggatttagat
     8521 aacgtatatt tagctacaga tgtagttgtc gtaccaagtg agcatgaggg tttttgctac
     8581 gcacttttag aaggagctgc aagagagtgc gctctcattt gtagcgatat ccctgggcct
     8641 gacgcaattg ttattaataa aagaaatggg attttaataa cccctaaaaa tattgatagc
     8701 cttatggtta gtatgtctga attatttaat gatagagaaa tgacaaaaaa atatggagaa
     8761 caagcatata tgatagcttc cgaatttgaa cgaaaaaaaa tcatgttata ttatagttac
     8821 tacataagaa gtttatttat tgaacaaagg tgatttttaa atgtaattat ccgtatacaa
     8881 ctaatactga tataattttt gtttaatctc attagcaggt attttatgaa aaagaaagct
     8941 ctgattactg gaattactgg acaagatggt tcttatcttg ccgaattttt attagataaa
     9001 ggttatgaag ttcatggcat aaaaagaaga gcctctcttt ttaacactaa tagaattgat
     9061 catatttatc aggatctaca tcatgagaaa caaagttttt ttcttcatta cggtgatctt
     9121 accgattcgt tgaatatagt tagattggta caagaaatac aacctgatga aatttataac
     9181 ttaggggcac aatcgcacgt agcagtatca tttgaatcac cagaatatac agctgatgtt
     9241 gacgcaattg gtacgttacg attgttagaa gctattcgta tatgtgggct tgagaaaaaa
     9301 actcgttttt atcaagcctc tacctcggaa ttgtatgggt tagtgcaaga aatacctcaa
     9361 aaggagacta caccatttta tccaaggtca ccttatgcag tagctaagat gtatgcatat
     9421 tggattacag tcaattatag agaatcttat gggatatatg cttgtaatgg aattttattt
     9481 aatcatgaat cacctcgacg tggtgaaaca tttgtaacaa gaaaaatcac ccgagcatta
     9541 gcaaatattg cgtacggatt agagccatgt ttgtatttag gcaatatgga tgctttgcgc
     9601 gattgggggc atgcaaaaga ttatgtaaga atgcaatggt taatgttaca acaagaaaca
     9661 cctgatgatt tcgtaatagc tacgggcaag cagatctctg taagagagtt tgtgagaatg
     9721 gctgctgcta atttaggtat tgagcttgaa tttattggtt gtggtttgaa agaagtagga
     9781 gttgtgaaag caattcatgg aaatgaagct ccttcggtta aaattggcga tgttattgtt
     9841 caagtggatc ctcgttattt tcgacctgcc gaagttgaaa ctttgctcgg ttatcctgaa
     9901 aaagccaaaa taaaactagg gtgggaacct gaaataacga tcgaagaaat gtgtaaggaa
     9961 atgataagaa atgatcttaa catagcaaag caacattctc tgttaaagtt aaatgggtat
    10021 gatgtgtcct tgtcagtgga gaaataatga aatgaaaatg aaaaaaatat atattgcagg
    10081 gcatagaggg atggttggtt ctgcaatcaa acgtcagtta atgaagaata gttctgttga
    10141 gttaattttt cgtagtagtc acgaacttga cttgatgaac caagatgctg tccaaaaatt
    10201 ttttaataca gaaaggatcg atgaagtcta tttagcagct gctaaagttg gtgggatata
    10261 tgcgaataat aaatatccag ctgactttat atataataat atactcatac aagccaatgt
    10321 aatcaattca gctcatagag ctggggttca aaaattatta tttttaggct catcatgtat
    10381 ctatcctaaa tttgcagaac aacctataaa agaagaatcc ctactttgtg gttcattaga
    10441 accaacaaat gaaccttatg cgattgcgaa aataacaggg attaagttgt gtgagtctta
    10501 taatagacaa tatggtcgtg attataggag cgttatgcct acaaatcttt atgggcctaa
    10561 tgacaatttt catcatgaaa attcacatgt attacctgcc ttaatacggc gtttccatga
    10621 agcaaagatt aataatcttc cagaggtcag tgtttggggt tctggcaatg cgttacgaga
    10681 atttcttcat gtagatgata tggcagctgc atccatatat ataatgaatt tacctaatga
    10741 acgttataaa gaatatacta aaccagagtg ctcacacata aatgttggtt ctggtaaaga
    10801 ttattcaatt cggcaattgg cagaaatggt tgcggaaatt actaattata aaggaaatat
    10861 ttactttgat agtactaaac ctgatggaac acctcgtaaa ttaatgagca atgaacgatt
    10921 gagtaaacta ggctggtcac ccaaaatcga gttatatgat ggggtaaaag atacttacca
    10981 gtggtttttg aataatatag aaaatataag aaaataaaaa tcttacttaa aaaattatgg
    11041 gtaggtccta ctggaagaaa tattttataa atgacattta tatttgtaca gaggttatag
    11101 tagatgcttt ttccgataat tatggctggt ggcaccggta gtcgactttg gccactgtct
    11161 cgagagtctt ttccaaagca atatctaaaa ctttatggtg acatgacaat gttacaatct
    11221 acaattgcga gattaaatac attggattgt tcgaccccat tagttatttg taatgaagag
    11281 catagattca tcgttgcgga acagttaaag aaaattaata aattagataa gaatattctg
    11341 cttgaaccac aaggtaaaaa tacggcccca gcaataacct tgtgtgcgtt tgctgcttta
    11401 aaatatgcaa aaaatgatga ggatccatta ctacttgtat tggctgctga tcatgtaata
    11461 ggaaacatat cttcttttac taagtcagtt aaaaatgcat taccgcatgc tcatgctggc
    11521 aagcttgtaa catttggtat aatacctaca acacctgaaa ctggttatgg atatattcac
    11581 cgaggagaaa aatttaatga tgcgtacaaa gtttcatctt ttacagaaaa gcctgatatt
    11641 tatcgtgcgc aagaattttt aaaaacaggt gaatattatt ggaacagtgg tatgttccta
    11701 tttagtgcta aacgactgct tgcagagatg gaaaagtatc gcccagatat tttttcagca
    11761 tgtgaacaag cttttttatc gccatctaat gatctagatt ttattcgtgt ggatcgtgat
    11821 aagttttcgt tatgtcctag tgagtcaatt gactatgctg tgatggagaa tacggatgat
    11881 gccatcgttg tacctatgga tgcagaatgg tctgatgttg gttcgtggtc ttcattatgg
    11941 gatatttcag ataaggatgt aaatgggaat gctgttagtg gtgatttgat tagttttaac
    12001 tcttataata attttattaa agctgaagat aaactagttg ctttggttgg cgttaatgat
    12061 ttagtcttgg tacatacgga tgatgctata ttagtgtcaa gcaaatatga agtgcaaaat
    12121 gtaaaaaaaa tagttgaagt ccttaaccaa aacggcagag atgagtcaaa aaaacataaa
    12181 gaagtatatc ggccttgggg tgaatatcat tatattacgg aggcagagaa atacaacgtt
    12241 agacgcgtac taattaatcc aggtcaaaaa atctcaacgc aaacacacaa ccatagatca
    12301 gagcactggg ttatagtttc aggaatagcg aaaattacaa ttaataagga tataaatata
    12361 attaaagaaa atgaatctat ttatatacct cccggtacta tgcataccat agaaaactca
    12421 gaaatgttgc cattagaaat aattgaaatt caaaccggtc aattattaac tgaagatgac
    12481 gttattcgtc cttaaacttt aatgtaaaag agaaatgaag atttgtaatt ataagtagtt
    12541 attaagctaa ttttaatgag gttttatgag caagaaattt aatactttat ctatcatcag
    12601 gaatagtggc gttgcctttg gtaccagtgg tgctcgaggc ctcgttacag aattcacccc
    12661 cgaagtatgc ggagcttttt ctcatgcttt tattaacgta atgaagcaaa agtacaagtt
    12721 tcatggagtc gcattagcaa ttgataatcg gcctagtagt tactctatag ctcaagcatg
    12781 tgcatctgtg attgcgcaat acggcttaaa ggtaaattat tacggtgtta tcccaacacc
    12841 ggcattagct tataaggcac tggaggataa aataccgtgt ataatggtaa cgggtagtca
    12901 cataccacat gatcgtaatg gattaaagtt ttatagccca attggcgaga tcactaagga
    12961 agatgaaatt cgcattaaag atgtttctat agattttaca ttggactcat ccataccaaa
    13021 tttatatccg tgtcctgacg cagctagttg ttattttaaa agatacattg acttttttcc
    13081 tgaaggaatt ttaaaaggta aaagagttgg cttttacgag cattcaagcg ctgggcgtga
    13141 cttatacatt cctatttttg aaaagttagg agcagagata gtttccatcg gtagaagtga
    13201 taaatttatc ccaatagata cagaagctgt tggtcatgat cttgttatcc aggcaaaaaa
    13261 atgggcgtta gaatataaat tagatgctat ttttagtact gatggtgatg gcgatcgccc
    13321 aatgttaagt gatgagaatg gaaaatggtt gcgaggagat atacttggac tgttatgtgc
    13381 tgaaacattg aatataaaat ctattgcgat tccaattagt tgtaactcat taataatgca
    13441 acgtaagtgt tttgaagaag ttgttttaac aaaaattggt tcgccttacg tattagatgc
    13501 tttttcttct ttaaaagata aatatcaaaa ttatgcaggt tttgaggcta atggtggttt
    13561 tatattaggt tcggatataa cagttgataa tagaaagatc aaagccttac ctacacgaga
    13621 tgcattactc cctatattag ttttactttc tgcaaataaa aaaatttcaa gcttggtaca
    13681 tagcttacca agtagattta cttattctga ccgaattgct aattttcttc cagaaaaaag
    13741 agataggtta ttcacaatat gtaaaaataa tccacataca atagttgatc ttttgggaga
    13801 gaatgaggcg ggcattgaag ttaaagatat aaataattta gacggttata gatttacact
    13861 aaataataat gtcatcattc atctaagatc ttccggaaat gctccagagt tgagatgtta
    13921 ttgtgaggct gacagtgagt tttgtgctca aaaatatgtg gagagaattt taagttcggt
    13981 tgcttgcctt ataaaaataa attaataatt taaggttgtt tagtttttag tgcagtttca
    14041 ccttttcatg actgcactat cattttatat caaaacttaa caagctagac agcaaggttg
    14101 acgaggcaat gcagaataat aaacgcgttg tatttatgaa tgtgtctcta aatataatgc
    14161 agcaactgat taatacggta tataccttta tcataccccc attaattatt ataaattatg
    14221 gttcggatat aaatggtttg atttcttctg ttaaacaatg ccttgtatat ttagctctcg
    14281 tagaggctgg tgttggaggg gcagctattg tagcactatt caaaccattg tcagaagggg
    14341 atcgtgtgct acaaagtcag atcatatcgg aggctaaata tttttactca aaaattggtg
    14401 ttgtatttat tattttactt ttattatttt caataatata cgcacaaata atttctgacc
    14461 aaattaacat cattctagca attagccttt ttgttattat ggggctaata aatgcggtgg
    14521 attttttatt aatatcaaag tatcaggttt ttcttactgc tgataataaa ttatatgtaa
    14581 cttcggctac aaaaatatta acaatcatat tagatatggg atgtgcttat attctgataa
    14641 ctgaaggtat gaatattatt ttggtgcaaa tttgctccgt gtttatctat atgattagat
    14701 atgttattta ttatttttat tttctgaaaa aatacagtta tatatcattt gatctaaaaa
    14761 ataaaactgc attgcttcat cagaaatcag cagttttaat tcatcagata tcaggagcat
    14821 tagttttcaa tagcccaatg atattgatta ctatttttct taacctaaag gcagcaagta
    14881 tatattctat ttatatgctt atatttatgg ctattaaggt ttttttatcg accattacca
    14941 atggtatgca atccgtattc ggccgtatga ttgttaatga aaacattaat gctgtaagag
    15001 aatatttttc aaagtttgaa attttatatt tttcatgttt gggatggttc tattcatgtg
    15061 ctttgatttt atcgtatgat tttatatatt tatatacttt aaaatttaac gatcctgatt
    15121 atgttcaaac tatattgatt tatctttttg ttattatagg tatagctgat aacattcgcg
    15181 tcccaggaat gacattaata gcggctaaag gtcactatga acaaacaaaa tacagatcta
    15241 ttattgaggc tttattaaat atcgtttttt caatagtttt tattaaaata tatgggcttc
    15301 ctggtgtatt gctaggttcc attatctctt attcatacag gacaatagat attatttatt
    15361 atagtagcaa taaaatttta aaacaatcga taatatttaa aatgataatg ttattttttt
    15421 tggttttttt cattttggct aattatttta tcgttgaaag tttgaaaatg ccatttttta
    15481 taaatgattt ttttaaatgg attttatatt caacttttgt tgcagttcta tcagctttgt
    15541 tatggttatt gttttataaa attatgaggc taatgaatga caaaacttat taaatttttt
    15601 atgcgtaaat tgtaactttt atattgcata ctggtatatt ttaaagattt tcatacaagc
    15661 cgctacttat atgcggtaac cccctgacag gagtaaacaa tgtcaaagca acagatcggc
    15721 gtcgtcggta tggcagtgat ggggcgcaac cttgcgctca acatcgaaag ccgtggttat
    15781 accgtctcta ttttcaaccg ttcccgtgaa aagacggaag aagtgattgc cgaaaatcca
    15841 ggcaagaaac tggttcctta ctatacggtg aaagcgtttg ttgaatctct ggaaacgcct
    15901 cgtcgcatcc tgttaatggt gaaagcaggt gcaggcacgg atgctgctat tgattccctt
    15961 aagccatacc tcgataaagg tgacatcatc attgatggtg gtaatacctt cttccaggac
    16021 accattcgtc gtaaccgtga gctttctgca gaaggcttta acttcatcgg taccggtgtt
    16081 tccggtggtg aagagggcgc gctgaaaggt ccttccatta tgcctggtgg ccagaaagaa
    16141 gcctatgaac tggttgctcc gatcctgacc aaaatcgccg cagtagctga agacggtgag
    16201 ccatgcgtta cctatattgg tgccgatggc gcaggtcact atgtgaagat ggttcataac
    16261 ggtattgaat acggtgatat gcagctgatt gctgaagcct attctctgct taaaggcggc
    16321 ctgaatctct ctaacgaaga actggcacag acctttaccg agtggaataa cggtgaactg
    16381 agcagctacc tgatcgacat caccaaagac atcttcacta aaaaagatga agacggtaac
    16441 tacctggttg atgtgatcct ggatgaagcg gctaacaaag gtaccggtaa atggaccagc
    16501 cagagcgcgc tggatctcgg cgaaccgctg tcgctgatta ccgagtctgt gtttgcacgt
    16561 tatatctctt ctctgaaaga tcagcgcgtt gccgcatcta aagttctctc tggtccgcaa
    16621 gcgcagccag ctggcgacaa ggctgagttc atcgaaaaag ttcgtcgtgc gctgtatctg
    16681 ggcaaaatcg tttcttacgc ccagggcttc tctcagctgc gtgctgcgtc tgaagagtac
    16741 aactgggatc tgaactacgg cgaaatcgcg aagattttcc gtgctggctg catcatccgt
    16801 gcgcagttct tgcagaaaat aaccgatgct tatgccgaaa atccacagat cgctaacctg
    16861 ctgctggccc cgtacttcaa gcaaattgcc gatgactacc agcaggcgct gcgtgatgtc
    16921 gttgcttatg cagtacaaaa cggtatcccg gttccgacct tcgccgctgc ggttgcctat
    16981 tacgatagct accgtgccgc tgttctgcct gcgaacctga tccaggcaca gcgtgactat
    17041 ttcggtgcac atacttataa gcgcattgat aaagacggtg tgttccatac tgaatggctg
    17101 gattaatctg atttaaccaa caataaaatt gaggcccggc gtatattgca ccgggctttt
    17161 ttttgccatt tatcttatag acttaatttc actgcttaat attaacttaa taaatatcag
    17221 ctattcttat aaagaaaatc tgaattgttt ttcgttgcgt tgaccatcga acaacgtagc
    17281 gttaaaactt ttagctctta tcaggatgct aaaaacatca tgattcacag ttgagttaat
    17341 tctgagagca tgaaatgaaa atcaccattt ccggtactgg ctatgtaggc ttgtcaaacg
    17401 ggcttctaat cgcacaaaat catgaggttg tagcattaga tattttaccg tcacgcgttg
    17461 ctatgctgaa tgatcggata tctccgattg ttgataagga aatccagcag tttttgcaat
    17521 cagataaaat acactttaat gccaccttag ataaaaatga agcctaccgg gatgctgatt
    17581 acgtcatcat cgccactcca accgactatg atcctaaaac taattatttc aatacatcca
    17641 gtgtagaatc agtaattaaa gacgtagttg agataaatcc ttatgcggtt atggtcatca
    17701 aatcaacggt tcccgttggt tttaccgcag cgatgcataa gaaatatcgt actgaaaata
    17761 ttattttttc tccggaattt ctccgtgaag gtaaagccct ttacgataat ctccatcctt
    17821 cacgtattgt catcggtgag cgttcagaac gagcagaacg ttttgctgcg ttgttacagg
    17881 aaggagcgat taagcaaaat atcccgaccc tgtttaccga ctccactgaa gcagaagcga
    17941 ttaaactttt tgcaaacacc tacctggcga tgcgcgtggc gtactttaac gaactggata
    18001 gctacgcaga aagtttaggt ctgaattccc gtcaaataat cgaaggcgtt tgtctcgatc
    18061 cgcgtattgg caaccattac aacaatccgt cgtttggtta tggtggttat tgtctgccga
    18121 aagataccaa gcagttactg gcgaactacc agtctgtgcc gaataacctg atctcggcaa
    18181 ttgtcgatgc taaccgcacg cgtaaagatt ttattgccga tgccattttg tcacgtaagc
    18241 cgcaagtggt gggtatttat cgtctgatta tgaagagcgg ttcagataac ttccgcgcgt
    18301 cttctattca ggggattatg aaacgtatca aggcgaaagg cgttgaagtg atcatctacg
    18361 agccagtgat gaaagaagac tcattcttca actctcgcct ggaacgtgat ctcgccacct
    18421 tcaaacaaca agccgacgtc attatctcta accgaatggc agaagagctt aaggatgtgg
    18481 cagataaggt atacacccgc gatctctttg gcagcgacta acatcttgtt atcagggcta
    18541 tttacgccct gattgtcttt tgttacttac gcaacaattc attattttta tcacttatcc
    18601 tatagcattc gcgaggatta tcgctaaact atgcggactt ggaaatttcc gtcagttagg
    18661 gtaatgatga gagtagaaaa taataacgtt tctgggcaaa accatgaccc ggaacagatt
    18721 gatttgattg atttactagt gcagttgtgg cgtggcaaga tgacaatcat catttccgtc
    18781 attgtggcta ttgccttggc tattggatat ttggcagtag cgaaggagaa atggacgtca
    18841 acagcaatta tcactcagcc cgacgtgggg caaattgctg gctataacaa tgccatgaat
    18901 gttatctatg gtcaggctgc accgaaagta tcggatttgc aggaaacgtt gattggtcgc
    18961 ttcagttctg ccttctctgc attagcagaa acgctggata atcaggaaga accagaaaaa
    19021 cttaccatcg aaccttctgt taagaaccag caattaccat tgactgtttc ttatgttggg
    19081 caaactgcag agggagcaca aatgaagttg gcccaataca ttcagcaagt tgatgataaa
    19141 gtgaatcaag agctagaaaa ggatctcaaa gacaacattg ctctgggacg gaaaaacttg
    19201 caggactctt taagaacgca ggaagtagtc gcgcaggagc agaaagatct gcgtatccgt
    19261 cagattcagg aagcgttgca gtatgcgaat caggcgcagg tgacaaagcc acaggttcag
    19321 cagactgaag atgtgacaca agatacgttg ttccttttgg ggagcgaagc gctggagtcg
    19381 atgattaagc atgaggcgac ccgtccgttg gtgttctcac ctaactacta tcagactcgt
    19441 caaaacctgc tggatatcga aaacttaaag gttgatgatc ttgatattca tgcttaccgt
    19501 tatgtgatga aaccgacgtt acctattcgt cgcgatagcc cgaaaaaggc aattaccttg
    19561 attctggcgg tgctgctggg cggcatggtt ggcgcgggga ttgtcttggg gcgtaacgct
    19621 ctgcgtaatt acaacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtaa
    19681 caccttgccc gttttttttg ccggatgcga caacaacatc gcatccgatc acccgcaact
    19741 cactgatgcc gtttacgcag attttcaatt accgtcgtta aatccagatc ctgatcctgc
    19801 aacagcacca gcaggtgata catcaagtca gatgcttcgt tggtcagttc aaagcggtca
    19861 tgcaccgttg ctgccagtgc ggtttccacg ccttcttcgc ccactttctg cgcaatgcgt
    19921 ttagtgccgc tggcatacag tttggctgtg taagaggttt ccgggtcggc agatttacgt
    19981 tcggcgagca gttgttccag ttgatacagg aacagccact ggtgagcggt gtcgccgaag
    20041 cagctgctgg tgcctttgtg gcaggtcggg ccgatgggat tcgccagtac cagtagcgtg
    20101 tcgttgtcgc agtccggggc aatgctcact acgttgagga aattgcccga cgtttcgcct
    20161 ttggtccaca gtcgctgttt agtgcgcgag aagaaggtga ctttgccgct ttcgatggtt
    20221 ttgtctaagg cttccgggtt catatagccc agcattaaca cttcgccgga taccgcgtgt
    20281 tgcacaatta ccggcattag tccgtcggtt ttttcccagt ccagttcgcg gcgttgttgt
    20341 tctgttaaca tatcctgatc tccacgccct gtgttgccag gtacgctttt aattcaccaa
    20401 tattgattat ttgtttgtgg aatacggaag ctgccagcgc accgtcaacg tcggcatcgc
    20461 ggaaagcttc gaggaagtgt tccatggtgc ccgcgccacc ggaggcaatc agcgggacgt
    20521 ggcaaacttc acgtactttt ttcagttgtt cgaggtcgta accgttacgc acgccgtcct
    20581 gattcatcat gttgaggacg atttctccgg caccgcgttt ttgtacttcc tgtacccagt
    20641 cgagcgtttc ccactgagtg acgcgggtgc ggctttcatc gccggtatat tgattcacat
    20701 gatatttacc ggtttcagcg tcgtaccagg tatcaatacc aaccacaata cactgcacgc
    20761 caaagcgatc cgccaggcga gtaattaacg tcgggtccgc cagcgcaggg gagttaatgg
    20821 agattttatc cgcgccaaag gaaagaattt tggcggcgtc atccagcgat ttaatcccac
    20881 ccgccacaca gaacggaatg tcgatcactt ccgccacgcg agatacccag cttttatcta
    20941 ccacacggcc atcgctggaa gcggtgatat cgtagaacac cagttcgtct gcaccttctt
    21001 cggcgtagcg ttttgccagc ggcacgatat cgccaatgat ttcatggttg cgaaactgta
    21061 cgcctttcac cacctgacca tcacgaacgt cgagacacgg aattatgcgt tttgccagca
    21121 tgcgatggcc tccttcacgg tgaatttacc ttccagtaat gcacgaccaa ctattacgcc
    21181 gcgtacacca gtaccacgca gggcagcaac atcgttaatg tcgccaatgc cgccggatga
    21241 ctgaaatgcc acctgcggat atctggcgca cacttcttca tataaagaga cgttagaacc
    21301 tgccagcgtg ccgtcgcgcg agatatcggt acacagcaca tgtttcaggc cgacgggcag
    21361 ataggtttcc accaattgtt ccagtgaaac gcccgagttc tcttgccagc cgctgaccgc
    21421 cacctgcttg ttgccttgct cgtcaatacg gacatccagc gccagcacta aggcatcggc
    21481 accgaagcgt tcaaaccagc ctttcaccat ttctggtgat ttcaccgcag tggagccaac
    21541 caccacgcgc gcaaccccag cttccagtaa cgccgccaca tcctcttcgc tacgcacgcc
    21601 gccgccaacc tgcaccggta cgttaacgcc cgccaccagg gttttaatca gcgggatttg
    21661 acgtttagcc ggatcttttg ccccggtcag atccaccagg tgcagcactt cggcaccctg
    21721 cgcggcgtaa tcctgtaagc gcggcagcgg gtcgttaccg taatcgcgct gtttgccgta
    21781 atcaccctga tggagacgca ccacagtgcc gtcgattaaa tctaatgccg gaataatcat
    21841 cacatctcca ggaagttttt cagcagctta gcgccagcgg aaccagaacg ctccgggtgg
    21901 aactgcacgc cgtagaagtt atctttttgt accgccgcgg tgaacggttc gccgtaatta
    21961 cactgggcga tggtccacgg attgaccggc attgcgtaac tgtgaacaaa gtaaaagtac
    22021 gcgccgtctt caatcccctg aaacaggcgg ttgcctgcct gcgggtaaac gcggttccag
    22081 cccatatgcg gcagtggcag accaaagtcg gtcattttcg gcacgtcttc gtcgatgatg
    22141 cccagcaagt cgacgccgtt gctctcttcg ctgcgccgcc ccagcaattg catccctaag
    22201 cagatgccca gcaccggttg ggtacaggct ttgatgagat caaacagctc gcgctcacgc
    22261 acctgatcca tcgctgcttg cgcagtgcca acgccgggta aaaacagttt atcggccagc
    22321 aacacgacgt ccgggtcacg gctgactttg ggttcataac cgtgacgcgc aatggcagat
    22381 ttcaccgagt tcaaattggc gcagccagta tcaaggatca ccacgttcat tacagcactc
    22441 ctttcgacga gggcagggtg tcgccttcca cgcggatggc ctggcgtagg gtgcggccaa
    22501 acgctttgaa caggctctct acacggtgat gatcgttttt acctttggtt ttcaggtgca
    22561 gcgtcacgcc catggtgtaa gagagtgagc ggaagaagtg ttcgatcatt tcagtgctga
    22621 gatcgcccac acgctggtag gtaaattcag ctttatattc cagatgcggg cgaccagaga
    22681 tatccagcgc gcagcgggca aggcattcgt ccatcggcag cacaaaaccg aagcggcaaa
    22741 taccgcgttt gtcgccaaga gctattttta acgcttcgcc cagcgccaga ccggtatctt
    22801 cgacggtgtg gtgatcgtcg atatagaggt cgcctttgac gttgatttcc atacggaaac
    22861 cgccgtgggt agcgatctga tccagcatgt gatcaaagaa gccaacgccg gtgttaatct
    22921 tgctgccacc ttcgcgatcc agccacacct ggacgtcaat ttgcgtctct ttggtgttgc
    22981 gcactacatg ggcgtaacgg tctcgtttcg tgagttgctc gccaatcatc ggccagttca
    23041 ggatttcgcg gtcgtagcgt aaaccattaa tgcccatgtt ttcagcgagt tgaatatcgg
    23101 tcgcgcgatc gccaatcaca taactattgg cacgatccat cgcttgctca gcgagataac
    23161 gctccaccag ttttactttc ggcttacggc agtcgcactc atcggcggtc aggtgcggac
    23221 aaatcagcac ttcatcaaac tgcacgcctt gcgaggtgaa gatctgcatc atcaggttgt
    23281 gcgggccatc aaaatccgcc tgcgggaaac tttgtgttcc cagaccatcc tgattagtga
    23341 tcatcaccag cttgtagccc gctttttgca gcttcagcag ctccgggatc acgcttggtt
    23401 caaaggcgag tttgtcaaaa cggtccacct gaaaatcact cggcggttcg ctaatcaggg
    23461 ttccatcgcg atcgataaaa agatacttct gactcatcaa acttgctccg cacgtaaggc
    23521 gtcaatgacg cgctggcttt cttcacgggt tccgacggta attcgcaggc agccgcttaa
    23581 agagggttgt ttattctgat cacgtaagat aatgccctga tcccacaaag atttaaacac
    23641 tgcactggag gctttaaagc gcgccagaat gtagttggtt tcagagtcga aaacctgctc
    23701 cacgcagggg atctcttgca gtgcggcaat caggtattcg cgttctgcaa taatttgcgc
    23761 cacccgttcg cgcatggcga cgatcccctg cgggcttaac gcctgtgcgg caatgtcggc
    23821 aaccggcgtc gagagcgggt agggggcgat cactttcatc agcaggttga tgacttcttc
    23881 gtttgccagc gtaaatccgc aacgaagccc cgccagagca aaagctttcg acagtgtgcg
    23941 caaaatcgcc aggtgcggat attccgccag ccagccagcc agcgatgcct gcgggcaaaa
    24001 ctcaatatag gcttcatcgg caaccacaat cgccttaccg cgggttaact ccagcagggt
    24061 gcgaaaatcc tgcgggttga tcagttgccc ggtggggttg ttggggctgc aaacatagac
    24121 cacttttacg ccatccagct tgtcggaaat gccctgtaag tccagttgcc agttgtccag
    24181 cgtcggcact gtgcggcact cgacgccaat ggtttcagcg ctgacgctgt acatgccgta
    24241 cgttggcggg cagtagagga tggcatcttt acctggttcg caaaaagcgc ggatcagcag
    24301 ttcaatacct tcgtccgcgc cacggctgac cagcacctgt tccggtttta cgcctgcata
    24361 ttgcgcgtaa ttttcaatca ccgctttcgg ctgacattcc gggtagcggt tgagcgtttg
    24421 ctgagtgagc tgaaactcca cggctgtggg gtattcgttg gcgttcagcc agacgtcgcc
    24481 gttaccgccc agacgacgcg ccgactgata cggcgtcagg ttgcggacgt tttcacgcgc
    24541 taaatcggta atagtcacgg tgctcatgct tgctccttaa gggcgttaac acgcaaagta
    24601 acggcatttt tatgggcggt caggcgctcg gcggaggcca gtgtttcaat ggtcgaagcc
    24661 agcgcggaaa agccttcttt cgagagttcc tgcacggtca tgcgcttctg gaaatctgcc
    24721 agcccgaggc tggaacaggt ggcggtgtaa ccgtaagtcg gtagaacatg gttagtgccg
    24781 gaggcatagt cgcctgccga ttccggtgac cagtcaccaa gaaataccga accggcgctg
    24841 gtgatgccat cgaccagttc gcgggcgttg cgggtctgaa tgatcaggtg ctccgggccg
    24901 tactggttgg aaatctctac gcactgcgct aaatctttag tcacgatcag gcggctggcg
    24961 ctcagtgcct gacgtgcggt ttcggcacgc ggcagttctg ccagctggcg ttcgacagct
    25021 tcggcaacgc gacgcgccat atcagcatcg ggcgtcagta aaatcacctg agagtccggg
    25081 ccgtgttcag cctgagaaag caaatcagaa gccacgaaat ctggcgtagc gccgctgtcg
    25141 gcaatcacca gcacttccga cgggcctgcg ggcatatcga tcgccgcacc gtccagacgc
    25201 tggcttacct ggcgttttgc ctcggtgaca aaggcgttgc ctggcccgaa gattttgtcc
    25261 acttttggca cagattcagt accaaacgcc agtgcggcaa tggcctgtgc gccgccgacg
    25321 ttaaacacgt cctgtacacc gcacagctgc gccgcataaa ggatctcatc ggcaatcggc
    25381 ggcggtgagc acagcaccac ttttttacag cccgcaatac gcgccggagt tgccaacatt
    25441 aataccgttg agaagagcgg ggcggagccg ccaggaatat acaaacctac cgaagccacc
    25501 gggcgcgtga cttgctgaca acgtacgcct ggctgagttt ctacatctac cggcggcagt
    25561 ttttgcgcag tgtggaaggt ttcaatattc tttactgcca ccgccatcgc ctgttttagc
    25621 tcgtcgctca ggcgttcgct ggcggcggca atttcctcag cagacacctt cagtgcggta
    25681 accgtggttt tatcaaactt cgcgctgtat tcccgcaggg cctcatcgcc gcgtgctttc
    25741 acgttatcga gaatatcgtt aacagtgcgg gtaatgcttt cagaagcgga gatcgccggg
    25801 cgcattaaca gctggcgttg ttgctccgca gtacagctat tccagtcaat gattgtgtta
    25861 aagctcatgg cgatcactcc atcatcttct caatcggcag gaccaggatt gaactggcac
    25921 ccagcgcttt cagtttttcc atcgtttccc agaacagggt ttcgctactg accatgtgca
    25981 tcgctacgcg ttgttggtca cccgccagcg gcagaatggt tgggcgttcg gcacctggca
    26041 gcagggcgat gacttcatcc agacgttcgg tcggtgcgtg catcatgatg tattttgatt
    26101 cgcgcgcctg aatcacaccc tgaatacggg tcagcagttt gtcgatcagt tgctgtttgg
    26161 attcttccat ttcgccatcg cgctggatca ggcaggcttt cgagcgatag ataacttcga
    26221 cttcgcgcag gccgttagct tccagcgtgg caccggtgga aaccagatcg caaatcgcat
    26281 ccgccagtcc ggcacgcggg gcgacttcaa cagaaccgtt cagtaagcag gatttaaaac
    26341 agataccttt ctggtcgaga taacgcttga gcaggtgagg ataagaggtg gcgatacgtt
    26401 taccgtttaa ggagagcggg ccatcccagg cttcatcaac cggcgttgcc agtgaaagtc
    26461 ggcagccgcc gaaatccaga cgacgcaggg taaagtagcg tggatcttca ccctgggcgc
    26521 ggcggttaag cagctcttct tccagcacgt tttcgccgat aatcccgagg tcaaccacgc
    26581 cgtccattac cagaccggga atgtcgtcgt cacgcacgcg cagaatatca atcggcatgt
    26641 tttctgccat cgcgatcagg cgctgggtgt gaagattgat tttaatgcca cagcgcgcca
    26701 gcaattcgcg tgagtcatca cttaaacggc cggatttctg catagctatg cgtaaacgag
    26761 tgttgtctgt cattttctta ttcctcttta aacctgtctg aaccgttatc aaccgcgcaa
    26821 taaaaaagcc cccggaaggt gatcttccgg gggctttctc atgcgttcat gcaccactgg
    26881 aagatctgaa tgtcttccag cacacatcgc ctgaaagact agtcaggatg atggtgatga
    26941 tggtggtgtt taaattgaac gcgtgtcata aaagtctctg tgaatgttta ttcaactgat
    27001 gtcttttaac ctaaaccact ttcacgttag aaagcaaggg cttttttata tattttatgg
    27061 caatggattt gtttaatgaa tgagcgggga aaggagggcg ctaagaggac tgtcctctaa
    27121 gctgacggca ccaaaaatag cgaagcctgg attttcgtct cactgacaat accttgatct
    27181 acaaactaat taataaatag ttaattaacg cccatcattg tacaatgagc tgtacaaaag
    27241 aggagattga catgcgtaca attagctaca gcgaagcgcg tcagaatttg tcggcaacaa
    27301 tgatgaaagc cgttgaagat catgccccga tccttattac tcgtcagaat ggagaggctt
    27361 gtgttctgat gtcactcgaa gaatacaact cgctggaaga gacggcttat ctactgcgct
    27421 cccccgctaa cgcccggaga ttgatggact caatcgatag cctgaaatca ggcaaaggaa
    27481 cagaaaagga cattattgag tgaaactaat ctggtctgag gaatcatggg atgattatct
    27541 gtactggcag gaaacagata agcgaattgt taaaaagatc aatgaactta tcaaagatac
    27601 ccgcagaacg ccatttgaag gtaaggggaa gccagaaccc ctgaaacata atttgtcagg
    27661 tttctggtcc cgacgcatta cagaggagca ccgtctggta tacgcggtta ccgacgattc
    27721 actgctcatt gcagcatgtc gttatcatta ttgaacagat tcattcatag aatgaattgt
    27781 cagttttgat acgctagcgt agccttatag atctaaggtg tatcaggaga taacggatga
    27841 aaaaggtcgc aattgtcggg ttagggtggt taggcatgcc gctggcgatg tcactttcag
    27901 cgcgaggctg gcaagtcacc gggagtaaaa ccacacaaga tggtgtcgaa gcgtcccgaa
    27961 tgagtggcat tgatagctat ctgcttcgca tggagcctga gttagtttgc gattctgacg
    28021 atctggatgc cctgatggat gtcgatgcgc tggtcattac gcttccggca cgtcgtagcg
    28081 gccccggcga tgagttctat ttacaagcgg tacaagagtt agtggatagc gcgctggccc
    28141 atcgtattcc ccgcattatt ttcaccagct caacatctgt ctatggcgac gcgcaaggca
    28201 cggtgaaaga aaccaccccg cgtaatccgg tgaccaacag tggacgagta ttagaagaac
    28261 tcgaagactg gctgcacaat ttacccggta cttcggtcga tattctgcgt cttgcggggc
    28321 tggtcggacc gggacgtcat cccggacgct tctttgccgg aaaaaccgcg cctgatggtg
    28381 aacatggtgt taatttagtc catttagaag atgttattgg cgctatcact ctgttgttac
    28441 aggcacctaa aggcggacac atctataata tatgtgcgcc agctcaccct gcgcgtaatg
    28501 ttttctatcc gcagatggcc cgtttactgg ggctggagcc tccgcagttc agaaatagtc
    28561 tggacagcgg caaaggtaag attattgatg gcagtcggat ttgtaatgaa ctgggatttg
    28621 aataccagta tcccgatccg ctggtaatgc cgctggagta aacgatcacg ccagcaggac
    28681 gccgcatgca ctgcaagggg gcgtgtatga aaccactgct ggatgtgttg atgatcctcg
    28741 atgctttaga aaaagaaggc agttttgcgg cggcgtcggc caaactctat aagacgccgt
    28801 ccgctctgag ttacaccgtt cacaagctgg aaagcgacct taatattcaa ttgcttgatc
    28861 gtagcggtca ccgcgctaag tttacccgta ccggaaaaat gttattagag aaagggcggg
    28921 aagttctgca taccgtgcga gaactggaaa agcaggcgat caaactgcat gaaggctggg
    28981 aaaacgagct ggtgattggc gtggacgaca cgtttccttt ttctctgctt gcgccactga
    29041 ttgaagcttt ttatcaacat catagcgtga cgcgcctgaa gtttatcaat ggcgtactcg
    29101 gtggttcctg ggatgccctg actcaggggc gagcggatat tattgttggc gcgatgcatg
    29161 agccaccatc ttccagtgag tttggttttt cacggttggg cgatctggaa caagtctttg
    29221 cggtcgcgcc tcatcatccg ttagccctgg aagaagagcc gttaaaccgt cgtattatta
    29281 agcgatatcg ggcgattgtg gtgggggata ctgcgcaggc aggcgcttct acggcctcac
    29341 aacttctcga cgaacaagaa gccattaccg ttttcgattt taaaaccaag ctggagctgc
    29401 aaattagcgg cctcggctgc ggctatttac cccgttatct ggcacaacgt tttctcgata
    29461 gtggcgcgtt aatagagaag aaagtggtcg cccaaactct ctttgaaccc gtctggattg
    29521 gctggaacga acagaccgca gggcttgcca gcggctggtg gcgggatgaa attttagcaa
    29581 atagtgcgat cgccggtgtt tatgcaaaat ctgatgacgg aaaatcagcc atttaaagaa
    29641 aaattattat gacaagcctc tcattctctt gtcatttccc ccccatttag gcacaatgcg
    29701 ccgctgtcaa aaaatgacta aaaaccgacg tttcatcagc gtcggttatt ttttgcttca
    29761 aaccaatcat tcataccaag aggccgggct tcgtaccgga tagatattta ctaaaaatcg
    29821 acagttgttg tcgctgagga atccaaaaaa atggggcaat tttttgctta cgcgacggtt
    29881 atcaccgtaa aggagaatga ccatgtcgca taacgttact ccaaacacct ctcgcgtgga
    29941 attgcgtaaa acgcttacgt tagttccggt tgtaatgatg ggtcttgcct atatgcagcc
    30001 gatgacgctg tttgatactt ttggtatcgt ttcaggcctc acggatggtc atgtgccgac
    30061 agcctatgcg ttcgcattga ttgcgatcct gtttacggct ctgagctacg ggaagctggt
    30121 tcgccgctat ccttctgctg gctctgcata cacttacgcc cagaaatcca ttagcccgac
    30181 cgtcggcttt atggtgggtt ggtcttctct gctcgactat ctgttcgcgc cgatgatcaa
    30241 cattctgctg gcgaaaatct attttgaagc tctggtgcct tccatcccat cgtggatgtt
    30301 tgtggtggcg ctggtggcct ttatgaccgc ctttaacctg cgtagtctga aatccgtagc
    30361 gaacttcaac actgtaatcg tcgtgttgca ggtagtgctg atcgcggtga ttctgggcat
    30421 ggttgtttat ggcgtatttg aaggtgaagg cgctggtacg ctggcgagca ctcgtccgtt
    30481 ctggtctggc gatgcgcatg ttatcccgat gattaccggg gcgacaatcc tgtgcttctc
    30541 ctttaccggc tttgacggca tcagcaactt gtcggaagaa accaaagatg cggagcgcgt
    30601 gatcccacgt gcgattttcc tgaccgcgct gatcggcggc atgatcttca tctttgcaac
    30661 ttacttcctg caactgtact tcccggatat ctctcgtttt aaagatccgg aagcatcaca
    30721 gcctgaaatc atgctgtacg ttgcgggtaa aacgttccag gttggcgcgc tgatcttctc
    30781 caccattacc gtactggcgt ccggtatggc ggcgcacgca ggcgtggcgc gtctgatgta
    30841 cgtaatgggt cgtgacggcg tattcccgaa aagcttcttc ggttatgtgc acccgaaatg
    30901 gcgtactccg gcaatgaaca tcatcctggt tggcgcgatt gccctgctgg caatcaactt
    30961 tgacctggta atggctacag cgctgatcaa ctttggtgcg ttggtggcgt tcaccttcgt
    31021 taacctgtcg gtcatctcgc agttctggat ccgtgagaag cgtaacaaga cgctgaaaga
    31081 tcacttccag tatctgttcc tgccgatgtg tggtgcgctg accgttggtg cgctgtgggt
    31141 taacctggaa gaaagctcaa tggttctggg tctgatctgg gcggctatcg gtctgattta
    31201 cctggcttgc gtcaccaaga gtttccgtaa tccggttccg cagtacgaag acgtagcgta
    31261 atcatctttt gtaataataa accggagtcg aatgaatatt gggctccggt ttttttattc
    31321 ttttttatcg acatcctcat taataaccat ttgttctaat ttagagaatt tggttattgg
    31381 tctgcattcc accagtcatg aataatttcc ctattaacat tcatctggtt aattaattta
    31441 tgttttcaat gatattaagc gggctaattt gtggtgctct gctgggattt gtcatgcagc
    31501 gtgggcgttt ttgcctgaca ggtggttttc gcgatatgta tatcgcgaaa aataatcgca
    31561 tgttttacgc cttgctgatt gcaatttcgg tacaaagcgt gggggttttt gcgttaattc
    31621 aggcgggcct actgacttac gaagccgggg cgttcccgtg gcttggtact gttataggtg
    31681 ggtatatctt cgggctggga attgttctgg cgggtggatg tgccaccggg acctggtatc
    31741 gcgcgggtga gggattgatc ggcagttgga tcgcgctttt cacttatatg gtgatgagtg
    31801 cggtgatgcg ttctccacat gtcagtggtt taaatcaaac cttgcagcac tacagtactg
    31861 aacataactc cattgccgag acgtttaatc tgtctgtgtg gccgttgatt gccgttttgc
    31921 tggtgataac tttctgggtg gtgatgaaag agttgaagaa gccaaaactc aaagtcgcga
    31981 ccttaccgcc gcgccgaacc gggatcgctc atattctgtt tgaaaaacgc tggcatccct
    32041 ttgtcactgc tgtactcatc ggtttgattg cgcttttagc ctggcccctg agtgaagcaa
    32101 ccggacgcat gtttgggtta ggaatcactt ccccaacggc caatattctg caatttctgg
    32161 tcgcgggtga cgtgaaatac attaactggg gcgttttcct ggtgttaggg atcttcgtgg
    32221 ggtcatttat tgcagccaaa gcgagccgtg agttccgcgt tcgcgcagct gatgcacaaa
    32281 caacattacg tagcgggtta ggtggtgtac tgatgggctt cggtgccagt attgcaggtg
    32341 gttgctctat cggtaatgga ctggttatga ctgcaatgat gacctggcag ggctggattg
    32401 gccttgtatt tatgattctc ggagtctgga ctgcgtcctg gcttgtgtat gttcgaccgc
    32461 agcgtaaggc gcgactggca actgctgcgg caaattaagt aaggtgtaaa gatggcgatt
    32521 aaaaagctgg atgtggtgac gcaggtctgt ccgtttccac tcatagaagc aaaagcagca
    32581 ctggcaggaa tggccagcgg cgatgagttg gtaattgagt ttgattgcac ccaggcaaca
    32641 gaagcaatcc cacaatgggc ggcagaagaa ggacatgcca tcaccgatta tcagcagatt
    32701 ggcgatgctg cgtggagcat caccgttcaa aaagcctgac ccaacattgt cctccgccgt
    32761 atcagtggcg gaggcttcaa actagacgat ctcttccgcg tactgccaaa gcgcttttaa
    32821 cagcgccact ttttctttat cgtcggcata ctgctgcgcc agcatctgaa tctcttctgc
    32881 atagccttgt aaaaactcgg gtgtgaatac ctggcggcgg tgctccagcc aacgttgctg
    32941 ctcggcataa tccagcgtcc ccgggaagtt gcgcgcccga taattgaaca acagcttttc
    33001 aatccgttta tcaacaaaag tgatatccag tgccggtaaa ttacgcggct cggtttccag
    33061 cacaattttc attgctgcac gatcggcgtc actgaaaaag ccgttataaa gctgtgcatc
    33121 cacgttatct gaaggcgtaa acggttcggc ttccgcgaat atcgccacca ctttttcgcg
    33181 cacttgcgga ttttcacgca gaattttcag gttattgagg caatgctgac gattaattcc
    33241 cagtcggtcg gcatcttccg ggcgtagcgt attcgcctgg gccagcaccg gacatttatt
    33301 gatatgcacc agcttaaccg gaacggcggc gttatcgcca agatcggttt ttgcggtata
    33361 taaacgctcg cgcaatgtgt cgctatccag ttccagtaat ggcgaaatgt ctcctgccaa
    33421 atccaccata attacggcat tgcgattttc aggatgccac gccagcggtg ccacccagct
    33481 ggtgttgccg cgccatgccc caaacattcc ggaaacgtgc accaggggtt tcatctgcgg
    33541 aacatcaatc aacgccatca gtttgtgttt attacgatgg gtaaagagat attcaaacag
    33601 gcgtggctga cgcgttttta ccagtttcgc catcgcaata gtggcgtaca catcagccat
    33661 cgcatcgtgg gcgttgctat gttcaattcc attcgctttg gttaaatgct caaggcgaaa
    33721 gctcggtaga ccgtcatcat tttcaggcca gtttattcct tccgggcgca gggcataaca
    33781 ggcacgcata acatccagta aatcccagcg cgagttatca tgctgccagc tccaggcgta
    33841 aggatcgtag aaattacgat aaaaaacgtt gcgtgtgact tcgtcgtcga aacgcacatt
    33901 gttgtagccc agaatacagg tcttcggtac gttaaaaagc gagtgaatac gggcggcaaa
    33961 cgcggcttcg ttttctcctt tcgcccgtgc ttcctgcggg gtaataccgg taattaatac
    34021 tgctccaggc tggggcaaat agtcatccgc gggcttgcag taaaagactt cgggttcgcc
    34081 gatgacattg aattcgctat cggtgcgaat ggctgcgaac tgtgcagggc gatctaacgc
    34141 ggggtgcgtg ccaaaggttt cgtaatcgtg aaacaaaaag gtagattgtt gcttaccgtc
    34201 attcatcatt aggttaaatc cgttatttct gctgtctgcc agagtatcaa atatcaccgt
    34261 gctaatcagc tttagcgcca caatttggca gctagtggca acagatcatg tcagataaaa
    34321 atgagagggt agtcacattt tcttgcactt tattccagcc agttcataag tatttccgta
    34381 aaaagaacag ctatttgaaa ctcctgaggg tttgctgttg aaacgccgtc ttattattgc
    34441 tgcttctttg ttcgttttta acttatcgtc tggttttgcg gcggaaaaca ttcctttttc
    34501 acctcagcct ccagagattc atgccgggtc ctgggtactg atggattaca ccaccggaca
    34561 gatcctcacc gcgggtaatg agcatcaaca gcgcaatccc gccagcctga caaagctgat
    34621 gacgggctat gtcgtggatc gcgctatcga tagtcatcgc attacgccag acgatattgt
    34681 caccgtaggg cgcgatgcgt gggcgaaaga taatccggtg tttgtcggtt cttcactgat
    34741 gtttttgaaa gagggcgatc gcgtatcggt acgtgattta agccgtggtt taattgtgga
    34801 ttccggaaat gacgcttgtg ttgctctggc tgactatatt gccggtgggc aacggcagtt
    34861 tgttgaaatg atgaacaact atgccgagaa gctgcatctc aaggatacgc attttgaaac
    34921 agtgcatggt ctggatgcac ctggccagca tagctcggct tatgatttag ctgtgctttc
    34981 tcgcgctatc atccacggcg agcccgagtt ttatcatatg tacagtgaga aaagtctcac
    35041 ctggaacggt atcacccagc aaaaccgtaa cggattattg tgggataaaa ccatgaatgt
    35101 tgacggcctg aaaacgggtc atacttctgg tgccgggttt aatctcattg cttcggctgt
    35161 agatgggcag catcgtctca ttgcagtgat aatgggggct gacagtgcaa aaggtcgtga
    35221 ggaagaggca agaaaattac tgcgttgggg gcaacaaaac tttactacgg tgcaaatttt
    35281 gcaccgtggg aaaaaggtcg gaacggaacg catctggtat ggcgataaag aaaatatcga
    35341 cctgggaacg gaacaagagt tctggatggt gctaccgaaa gccgaaattc cacatatcaa
    35401 agccaaatat acccttgatg gcaaagagct caccgcgcca attagcgccc atcagcgggt
    35461 aggggaaatt gaactttacg accgtgataa acaggtggcg cactggccgc tggttaccct
    35521 ggaatctgtc ggggaaggca gcatgttttc tcgcctgagt gattatttcc accataaggc
    35581 ctgacctttc ttttgcagca gactggcagg agtgcgagtc tgctcgcata atcaacactc
    35641 attccttgtg gttttaatat tgcaactata ctgtatataa aaacagtatt aatggaggcg
    35701 tcatgaacta cgagattaag caggaagata aacgtaccgt tgcaggtttc catctcgttg
    35761 gcccgtggga gcagacggta aagaaaggtt ttgagcagtt gatgatgtgg gtagataaca
    35821 aaaatattgt gccgaaggag tgggttgctg tctattacga caatccagat gaaacacccg
    35881 ccgaaaaatt acgctgcgac accgtcgtga cggtaccgaa taactttacg ctccccgaaa
    35941 acagtgaggg cgtcattctg acagaaattt caggtggtca gtatgcggtt gcggtggctc
    36001 gtgtagtcgg tgatgatttt gctaaaccct ggtatcagtt ctttaatagc ctcttgcagg
    36061 acagtgctta tgaaatgtta ccaaagccct gcttcgaggt ttatttgaac aatggcgcgg
    36121 aagatgggta ctgggatatc gaaatgtatg ttgcggtgca gccaaaacat cactaattca
    36181 tctcagggcg gtgtgttgac gcgatgacca ctcttttttt tgaaagcgaa aagagtaaga
    36241 tgcgcctttc aattttttcg ctcctgccgg gaaattacac tgttcccggt ttgtccgtcg
    36301 gataattcag aggcgcgcct tctggccgac agatgagtta tgagcgcttt taatcttcat
    36361 tacggagttt ctgcgtgcgt gccgataagt cattaagccc gtttgaaatc cgggtatacc
    36421 gccattaccg cattgtgcat ggtactcggg tcgcgctggc attcctgctc acttttctca
    36481 ttatccgcct gtttactatc ccggaaagca cctggccgct ggtcaccatg gtggtgatta
    36541 tggggccaat ctcgttctgg ggtaacgttg tccctcgcgc cttcgaacgt attggcggta
    36601 cggtgttggg ttcgatttta ggtcttatcg ctctgcagct ggagttaatt tcgttaccgc
    36661 tgatgttagt ctggtgcgcg gcggcgatgt ttctttgcgg ttggctggcg ctgggcaaga
    36721 aaccgtatca aggattattg attggggtga cgctggcaat tgttgttggt tccccgacag
    36781 gtgaaattga tacggcgtta tggcgaagcg gcgatgtgat cctcggctct ttactggcaa
    36841 tgttgtttac cggtatctgg ccacaacggg cgttcatcca ctggcgcatt caactggcga
    36901 aaagtctgac cgagtataat cgggtctatc aatctgcatt ctcaccgaac ttactcgaac
    36961 gcccacgtct ggaaagccat ctacaaaaac tcttgaccga tgccgtgaaa atgcgtgggc
    37021 tgattgcgcc cgccagcaaa gaaactcgta ttccaaaatc gatatatgaa ggtatccaga
    37081 ccattaatcg caatctggtt tgtatgctgg agttgcaaat caatgcgtac tgggccacgc
    37141 gccccagcca tttcgtgtta ttgaacgcgc aaaaacttcg tgatacccag catatgatgc
    37201 agcaaatact gctgagcctt gttcatgcgc tgtacgaagg taatccgcag ccggtttttg
    37261 ccaatacgga aaaattgaac gatgctgtgg aagagctgcg tcagttgctc aataaccacc
    37321 atgacctgaa ggttgtggaa acaccaatct atggttatgt gtggctgaac atggagacgg
    37381 cacatcagct tgagttgcta tcgaatctga tttgccgggc cttgcgcaaa taattcctga
    37441 acttcagaat catcttgctg ctgcttcgat tcagcaagga taaagggtat gatagtgaaa
    37501 agggataaaa gcattgtcat ctgcggcagc tatgagtaat gttggcccta acgaatagcg
    37561 gttgcttaaa cgaatccgac tctcacatta tcaggggtat aaaaatggaa actaccaagc
    37621 cttcattcca ggacgtactg gaatttgttc gtctgttccg tcgtaagaac aaactgcaac
    37681 gtgaaattca ggacgttgag aaaaagatcc gtgacaacca gaagcgcgtc ctgctgctgg
    37741 acaacctgag cgattacatc aagccgggga tgagcgttga agcaatccag ggcatcatcg
    37801 ccagcatgaa aggtgactat gaagatcgtg ttgacgatta catcatcaaa aatgccgagc
    37861 tctccaaaga acgccgcgat atctccaaaa agctgaaagc tatgggcgaa atgaaaaacg
    37921 gcgaagcgaa gtaattcctg ttttattcaa tgagggttgc ccggcaaccc tcattgctca
    37981 ttgattcttt tctgtgtatc accgtcatca ttctcatctg agaacctgtc gaaattagcg
    38041 acagacatct tctgtatgca gcaaggcaaa aagttctgta actccatggt tattaaccgc
    38101 attggttact aacacgttgt gcgctccggc ttcccgtaac caacttttca ccaaagatat
    38161 ttgttccatg ctggctaaat ctgctttggt tactactcca atgaccgggt gattcatggc
    38221 ccggtagggc gtttttacct ctaattcctg cttcagcgct gagattcctg cttgttgccg
    38281 gaaaagcagg cacctgccgt ctgaacggta tcgatccgga agcgtatctg cgccatattc
    38341 tgagcatact gccggaatgg ccctccaacc gtgttgacga actcctgcca tggaacgtag
    38401 ttctcaccaa taaataagcg tcaatacggt gctccgttga cgcttacttt aatccgtttt
    38461 ctgagcttag atttttctgt tcctacccag accgtggatt tggaattcat tttttgtcag
    38521 gtcaatgcta atcgttaaaa cggccataac gcacctccac tggaaggttt atcagtaata
    38581 tcctaccgtt gcttcatggt cggggcgtcc attccattat tatcggtttt cctttctgtt
    38641 tataaatgaa aacgccagct gtattcaggc tggcgtcagg ggaaatgaag cctgttgagt
    38701 gagattcacc ggttctggtg cagaagctgc agatggcgca gggtaaggac atcttctctt
    38761 gcctgcttaa tacaggacaa cagtgctgtt tctgtggtta atggcactat accctgcatc
    38821 actgcattgt cttacagcaa ctggtgaggg ctggcataag ttgccggtgt ttcggtagag
    38881 tcacggtcat ccaccacgcg gagtgaataa cctttttcag cggccttatt aatcagttca
    38941 gtgatattaa caccatcaat gtcaacgaca atgtgcccca tatccagcgc ctgtacgtta
    39001 acgctgtcgg cttccagtgt catcatttcg cctccggata cttacccagg gtaatgttat
    39061 ttaccgttct gtaattgtcg cgggtcatca ggccggtcgc cctgcgagcc cggaggatat
    39121 cgatgctgtt tattaactga gagcgggtac aggcgctgaa tcccggctgg tcggtagcac
    39181 cagcgcgtat ttttccacga gaaagttcac cgcatcacac agtgaaatgc ctgcctcaat
    39241 atgctgctca atcacacgtt catcggcaaa aggtgtgtca ttcagtgtga ggccatagtg
    39301 ctggtccagc agtcgggaca gcagtatctg ccagatttca acaggagacg ggcgagaact
    39361 ggtcgcctgc ccgggtaata caggtaatgt tttcatactg aagattttcc tgatatgcag
    39421 atataaaaat ggaaaagtgg cgtggtgaaa acaccaggcc gtagcagaag gctattctgg
    39481 agagttaatt tttcatttcg ggcgtcggat aaacagccag ataaacgtaa ccacaactgc
    39541 tgagggtatc ggctttgcag gtcagccctt ttgcatacag cgtgacggta tgctgatggc
    39601 ggggattcag ttcaccgctg gtgagcatga gttccagttg tttcatcagc agcggaaagg
    39661 cctggtccag gtggtacgca tctgcatcgc tgaacaggcc tctgataccg gcgcggtcgg
    39721 caaggtaatg caaccggtta ccctcctgca ccagacgtgc cccgaaacag ggcgtcacgg
    39781 tgcagggcag cccccaccag gggcggtcgt gattgtcgtc gggaagtgtt gtcccgggga
    39841 gtgtgtctga cacgataaaa tccttacaga aaatcggcta agaatggtcc ggtagtggcg
    39901 ataattctgc tcatcagaat tcccactcag ttcagggtga cgctcatcag ccggacatac
    39961 ggaccaaaac tgtccttacg gcgttcagca aacacggcca gcacaccggg aatatcctgc
    40021 acttcacgac cggtatacgc ttcagcactg ccgtgccagc ggtatttacc ggtacagaac
    40081 ggaaaaagac gggatgtcgg atgttgttgg tgaatacgca tggcttcacc acgggtgata
    40141 attttcataa taggatacct ctgaagacgg aagataaaag tgaaaacagg tgtgatgtga
    40201 ttgtggcggt gacgggttaa agcagaccgt gttccgcaaa ggagaaaacc tggctgccac
    40261 cgactatcag atggtccggc acccggatat ccaccaggcc cagtgcctgt accagacgtt
    40321 ccgtgataag gcggtctgcc ttactgggtg tgacttcacc ggaggggtga ttgtgtgcca
    40381 gcaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg ggatggactt
    40441 ccgtgcggtt gatggtgccg gtaaagaggg tttcaccggc aatcagttga ttctggttat
    40501 tcagatacag cacccggaat tcttcacgct ccagtcccgc catgttcaga atcagccatt
    40561 cccgtgctgc acgggtggag gtgaaggcca cgccgggttc atgaagatgg cggtccaggg
    40621 ttttcagggc ccgttgaatg aggctgcgct cgccgggcgt catctctccg ggcagaaagg
    40681 aaagttgttg cattgtgctt ctctccattc agtcgatgat gcgcataatg gcgctgcatt
    40741 ccggatgctg cagggcgtaa tcccgcaacc ggtaataatg gaccgtcatg gcataattct
    40801 ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc ggcaataccg gcggcttccg
    40861 ggctcatttc tgcgcggtta ccgttcatgg cattgaacag tacccatgtt tcgtcatcat
    40921 cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt cagggtgtac agattccaga
    40981 taccaccgca gtagtcttcg cacagacggt ccatccagcc gaagacacgg ggctccaggg
    41041 tcacccactg tggaatgagg ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag
    41101 ggacgaggct ggcaaccaac tgaggcggat tattctctgg tgttgtagct gaataaattg
    41161 tgggggtatt ctgagaaacg gttttcatga gataactcct taaataacaa ataaatgtgt
    41221 gtttttcagg aacgaaagct gaatgattcc ctcactcagg gaaacggcat cagcgcaggc
    41281 tctccagcat cgtttctgcc atcacccaca atgcgcgatt gagcttaatg tcggtatcaa
    41341 tgctgtgaat ggcccgggta tggatacgtt ttccttttgc actgcgaccg gaaatcccgc
    41401 ctttcagcat attctcctgg atggtctgat atgcgctcca caggtcctta ccgtaatcct
    41461 cccggcgtcg cggcgtcaga atgtcggcgg tggtgacggg ctgatgttcg tcaccataac
    41521 ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg tggcggcagg accagcgact
    41581 gcatggcatc acgcttctcc tctatccggt caaacacgcc caccacctcg taagcccctt
    41641 cgataacttt ctccactaca tttccccggt gtggaacacg cacttccccc agagactggc
    41701 cacagacgca cccgttctgg cagacgaacc tgaagtaacc cggcagcatc tggtagctgg
    41761 aggtaccgtc atgagagttg agcagaataa tttcagggac atgttctccg tttatctctc
    41821 cggcccgccg cagacgcagc atgtgttttg tgtatccccg gcggcccggg tcgcgcacac
    41881 gggtctggca ggcgaagaac ggctgaaagc cttcccgctg cagattttcc aggacggtaa
    41941 tggtgggaat gtacgcatac cgttcactgc gggaggtgtg ccggtcttcc ccaaaaatac
    42001 tgggtacatg gcgtatcagt tcttcatgtg tcagcggacg gtcacggcgt atctggtttg
    42061 cagcataacc aaaacgactt gctaatcgca tactttgctc cttatcggtg gttaagattt
    42121 actggtgcaa taaatgaaaa aagccgcgtc tcccggagaa gacgcggcct gacgggtgaa
    42181 atgaataagg tttattgtct gagaggcctt tcactggcga gccacgcatt aagcggcttt
    42241 ccggtaacaa tggctggggc tgtatttacc tttgatatta ataatgtgca gagaaacgca
    42301 tggtttactc cttagatttt aagtttgtcc tgggctacaa cgacaggttt tccggatgtg
    42361 tcgatgactc ctaccgtcgg tacgccattt ttgatttcga agaacggttt gttgctacct
    42421 gagcacagtt gattgtcccc gtattcaatc atttgtgctt tcccggtttt caggttaact
    42481 accgagacag tgtagtcgta gcaccaggca ccaccacgtt caagcacgaa aacatagctg
    42541 ttatctttat agttgaactg attctttgtc tggcaattca taccgtcaat ttcggtacag
    42601 gaaaacgctt ctgtagtatt aacgtatttg atgttatttg gcagttttac ttgtttcgca
    42661 gtagcgaaga cagagaacag aatagttaca gcaacgagta aagttttgat aaaagttttc
    42721 atgatagact ccttaaatta gttaaccgca gctacgtagt ttttcaccag atacgcgaat
    42781 tcatgcacgc caatttcacc gaaccaaacc aggtaggccg ggtcatctaa accaaagtaa
    42841 cggtagccaa caacttcatg ggtttttggg ttatagagtg ttacgataaa gtcccagccg
    42901 caggtattgg ctttgcaacc gtcgccctgg aaccagactg taccgttaat gtttactggc
    42961 gtgatacctc caccagggcc atccatatag ccgcctttac cgaacatgcc gctcagtccg
    43021 accggtttta atgctttgtc gatagcggct ttgtaggtag cgtcaccttc gtacaactcg
    43081 tacgttgatg gacacgctcc aggttgttca gcccaggctt ccgggttaca actaagagct
    43141 tgttttacgg tagccgcttc tgcgttaaag ttaaagcccg tgaacagaac cgccagaaat
    43201 aacataaggg tatgtaaacg tttcattgta aatattcttc cgttgtaaat ggcgctggaa
    43261 aatttctcat cagtgaaacc ttccggaagt ttccccccct tgtatgatgt atatcgccag
    43321 tattttattt catcccggct gtaattttta tattccccgg cacgtgcaat aacgctgacc
    43381 agagatataa ggctcattaa taactttaca ggtaatttca taattaaatt tccagtgagt
    43441 gaattgacag gtgaggggta aaactttctc tcaggtaata tatatctgaa aatattttca
    43501 ataacaccag acaggaaaaa caccaccaca ggcggtggcg tttctttggg gcgtctttat
    43561 ttaatccggg ccatcgcgtt tctcagtgcc aaatctgcct cttccgttat atccaggctt
    43621 ttctgcaata ccgcctctga ccgggtcagt gcctgacgtg ctgccagcgg tttccggata
    43681 tgctccatcg ccagcatcgt gtcttcatag ccctgctgac gccaggcgtc ggtatgtgcc
    43741 gatgtaaaac tgagcagacc gccgctgttg ccaccatcac ctgcatattt cagccttttc
    43801 cggggacgga tctcaaggat tgtggtgtcc ggaaaagcct ggcgatccca taaagaaccg
    43861 tcacacagat gcgtcacaat caccatattg catccggcat cgaccagagg cgtcacaggg
    43921 gtatttccct gcatatttcg ccatcctccc ataccaccat caccgaacat tgtcccctga
    43981 acctcacggg gacggaacag caggggcagc gcggctgacg caagcagagc ctctttctgc
    44041 tgtccgcggg gcaggctctg gatatgctga aaaacggcgt ttttcgtggt tccgacaccc
    44101 agttcagcac gaatgcagtc aataatatcc tgcatgcccc cttctgtggg gtacagcgac
    44161 acatacagcg gtagcccgtc tgccagagca tcagtatcaa gataatgatc catcagcgct
    44221 gtcaggggct catctgacaa caacggctga gccatcagat tgtcaaaccc gttgattgtc
    44281 gatatatggc gaagaagcgt cgtaagcagt gctcctgcac gtccggggat ctggcagagg
    44341 ttcattgcct ggaacaattt tttcagcaat gaaaagtaaa ccaatctgtt taccgacagc
    44401 acctgattat tccccagatg atcccagagc gcctccaggc gtacggcagc ttctgacaga
    44461 tcgggagagg ccgcgataat ggcaccattg aatgcgccaa tgctggcccc tgaaaccatg
    44521 ctgatctgtg taccacactc tgccagtgcc ttaaccactc ccgcctgata agcgcccacc
    44581 gccccgccac cggacagcac cagccccgtt ttcatttctg tactcatgca gcccccttct
    44641 gaaatatttc atcgaccata ctttcagaga ggcgttctgc agagcagagg cgaggcatgc
    44701 gaaccggtaa gtgttctgca gcctgcgtgg ttgaccagat caccggatgc tgctcttcac
    44761 tgcaccagtc tcccagccag ttaatcgcgt catgccactc gttatctgcc tgctcccggc
    44821 cctgaccacc aagccagtcc agagtgttca gacgcacggt tgcactaccg ccactctcct
    44881 gtgccatctg cgttaatgct ttatgtctga gaatatccag tagctggtta acctggcctg
    44941 tttcgctctg acgtatcaca tcaccaaacg cgccccagcg aagctcttca agtgccacat
    45001 aacaacgtcc ggcaggggaa aatgacgcat atcgcccggc gctccaccat gagaatatct
    45061 gctcaagatg taactgcgcc cgctgaacct ggtcaacacg gtggagtttt tcttcaagat
    45121 gattcagttt atggtgaacc tgatcggcca gggtgagtaa ctgctcccgc gtatctgcgg
    45181 aataatgcgc cagcctggct gtatcactga ccagtgaact gaccctgtcg tttacctggg
    45241 taatcgcgta gtttgtggtg gcaagtgacg ttgtcatctc cgtcagccag cgcagggacg
    45301 cctcaacgcc ctgtgcaagc gacgcattga tggcattctg ccgtgcagcg ccttcgccgg
    45361 tcagcccctc ttttaaacgc cggaagaacg tgcgctctcc ggccgtgcgg atatgctcct
    45421 gtgcaacatc gattccgttt gcgaaatcaa ccacaaattt ttcaggaaga caggtgagta
    45481 cctgacatac ccggttatca gcaatttgtt gaataaatgg tgaagtcaca gcattgtctc
    45541 cagctcttcc ctcagcgcct tactgtcagt aatgagctca ggaacccttt catttaatgc
    45601 ctgaagtcgc ccggtaagtc gttcctgcgc ctgctgatcg cgtttatgat cttcagagct
    45661 ctgaatcagc gtgttacgca gttgttcaac tttctccctg aatgcgcaga aaaaggcatc
    45721 gatttcctgg cggacgggct ggttaatatc ctgctcaatg gatgcctgca gctccccgaa
    45781 atatgcccgg gtcagtgaca taacttcctc tctgaccttg ttgatattga tcacgctgcg
    45841 actcacatcc tcgtcatagt tttcccagcc caggtcactg gtgccaaacc atcgacaaac
    45901 ggttccccat acgccactct gtcgacggcg acgggtgacg gtctccgttc tttcctccat
    45961 aagcgcactc agatttattt ttaccgcgag ccgggtctgt aactggctga ctgccggaaa
    46021 actgattttt acggtaaagc cctcatcctc caggcgggca ttgatgcgac cggcaatgtt
    46081 ttctgtggcg tacagggccg tgccacgaaa acctgtttca atcccgctga cgattttctc
    46141 cagctcaggc cggaactgtg cttcagcctc acggtgcagg ctggtcacgg tcgattcgat
    46201 ttgcgtcatc agttcaaggg cttccctgcg atcactgaat tttatttcgg ggctgtccgg
    46261 ggcaaaatct cgtttcctgt aatcctcccg gccctggttg ttgataccgg accattgaga
    46321 aattttcccc cataatccgg actgagacct tcgttgttct gcgtcttctt cctccagcat
    46381 ttgctgttct ttgcgtttac cgcttctgaa atagtcattc agctcctctt ccagtgaggt
    46441 gcagaccccc gtcagtaatt cccgggtttt agtatcgatc tcctccatgg ccattctgac
    46501 atcagtggtc atctgctcct gacagtccgc gatttcccgg atatccgcaa gcaggctggt
    46561 gatatgcgac tgcagtgact gaatgctttg ctgtagcccc tgatggcgta acaacaggta
    46621 ttcactgata ttttctgcat tctgcatcag ttttgcggcg gcagagtcaa ccgccagtgc
    46681 cgcagctctg gaatgtgagc tctgtatgac ttccgttatc agttgatcga taagtgagcc
    46741 ttcccacagg tctgtcgccc cttcagttgc cagttcactg tctttacaga cgtattcttt
    46801 tttcatgcaa ccgaaggcac ctctgataaa atcatcgacc caggcttcat caacaggaag
    46861 ggttccgttt ttccgtaact catgaagcgc acggttagcc aggtatgcct ggcgtgagga
    46921 tccgggataa acgcgggagg cgggcagcac atcgctgttc agcattgccg gaactttctg
    46981 gcgtacggca tctgccccat cgccattgcg gtctttctca tcaaatttat tgaccagtac
    47041 aaacaggcgg ccggctgata cgtcagcaat ggcattaagc tctttacgga cgtcttcatc
    47101 cgcttttgag ttcatctggg tgtaatccat cacagccaga accgcagagg ctttctgcag
    47161 ttgatcccgc atcatcactt ccatctgcgg ttgtccggcc tcattagggc cgggggtatc
    47221 cagcagtgtg agtgttccct ggcatgcatc catccccacc agatggctga attccacgtc
    47281 gatcaccggc agtttctgca cttctgcgta ttcatcaaaa ggaaattcag tccccattgc
    47341 cgcagcaaga cgaaccagat cgtttaacga tgccagtccg gtaaagattt cctcctcccc
    47401 gtggtattca tttttgagcc agccatcatc cgtcagaata tccagcagtt cgcgggtatc
    47461 cccggtttgc tgcagggtct gtgcgacctg ctgtcctgcc ggggtggcga gtttttcctg
    47521 cagtgtgatt aataaattgc ggacaggctg aatatgttcc agatggagaa ccggctcagt
    47581 ttttccggga acgtggcgga tgagcgtcgg tacagaggtc atggggcggt tacggttagg
    47641 cagaatttcc tgccccacaa tggcgttgat ggtggttgat ttgcctgctt tcatcgtgcc
    47701 gacaacagcc agcaccatct ccctgcgggc ggttttgatt tgttcgccct ccagctcctc
    47761 aattcttttc agtgctttct gtttatcaaa aagcagtctg ttttcgttct tactgtcaga
    47821 taacacatct ggctcctcaa ccatttgccg gagcagatta atgttcagtt gcaaaagtcg
    47881 gtcggcttca tcacaaagca gggcgatgtt cttttcgtgc attatttaac cttcctaaga
    47941 cgcattttca tgacaaccac ggaggaggtc atttactaca aacgatcgtt tataacgatc
    48001 gatacgatag taacgatcga tgtattctat taacttgaga aacacaacgc aaggggacaa
    48061 cccgggtatg gctgcgtttg catagtgttt tcatggagtt aaaaatctgt aacaacagcc
    48121 tggtacatca ccaccgggtt cagaagaaaa tccagttcca taccgcgcgg gcaacagaga
    48181 ccaccgagtc acggacggcg cgcagaaccg tgcgtgcaac agaggcaatg cagacgctct
    48241 ccgccgtgtc aaatatccgg tccaccgcac cggtaaactg ttcacgggcc tgagaccgga
    48301 cagactccgt gcgcagctcg tcctgcaggc gggtcatcag gggactggcg gcatggtcgg
    48361 gaagtgctgt catgagcgca ctgaccagcg tatccagttc ccagccggtg cgggccgata
    48421 cggcaacaac ccgatgtacg ggccggaaca gacggaatac cgcctccgtt ttttcgcgaa
    48481 tgttctgtgc ctctgcggga gaaggctgaa taccggccat atcccattca tggcagggct
    48541 ccgttttatc ggcctgcatc accacaaaca gtacccgctg atgtccccgg tgcaggatgt
    48601 gtcgccagaa atactcatcc acagacaggg cacggtcatc ggctttaatc agccacagta
    48661 ccaggtccag ttcgagcaga atgtcacggt acagggcttc atactctgca tccctgtccc
    48721 ggctctcgcc caccccgggc aggtcagtga taaccatgct gtgaccatgg ccattcagac
    48781 ggaagcgctg cacttcccgg gtgccggcgt gaacatcact gaccggggtg acctccccct
    48841 gaaacagcgc attacagagt gaggatttac gggcccgctt ttacccataa tgccaggcac
    48901 gggttcgtga ctggtgagtt tgcgcagatg ttccaggatg tgacgggaaa gtgagtaagg
    48961 cagggaggag agcggttttt caattgcctc aatggcatca gacggattca tacatgtatt
    49021 cccggtgaaa aaagacaaaa ccccgcgcac cggaaaggtg gcgggggatt cacggaaata
    49081 atatctgtct gaaattatct ggaatcgatt tggctgttct gcctggttgc caccaaatca
    49141 tggtacgcct gctcaatcat ttcattcagc agtttttttg ggaggacacc acgacttcgg
    49201 gcaatacttt caagctgttt gtggattttt ccggacagtt tcagagggac ggaaccatca
    49261 gcacgttcat cacgcctttt ttgttgctcc cgggcacttt tcagctcatc aagaagaatc
    49321 gctttatgct gaatcctggg ggcctggaag gagacctgcg taaacttcgc tctgttccgt
    49381 ctgggattac tttccagtaa aaactgtacc tgttgctcat tccagccctc ccagttatca
    49441 aacgtggcgc aggcaaacca gtatttctgc tccggggtaa ggggattcag cggggttccc
    49501 acacaccgga cctgcatggc gttccataac cagtcacact gttcactgtc cctgctgtcc
    49561 acccacgaaa agggggaggg gtaatcattg tttatgctgg cccattcaga gaatactgag
    49621 ttgataatcg tcctcgtagc ctctggtccg gatttcagca ggtaactgga gcagaaccat
    49681 ataatttcac ggtagatttt atccggatgc acggtgcgca tcggcttttc tctgggaccg
    49741 gcaatcttcg gttgcgatgg aaaccccgtg gcaaaccgca gatgttccgg gggcattcct
    49801 gaacgttttt caggaatggg tgtgagtcct gcgtttctgg tcttttccgc ttgtccggta
    49861 ttgcctccgg ccggagtgtt gtcgggagta tcttccggag gcgaggtgtt ttcattagtt
    49921 ggtggaacaa cagcagaact gtccatctcc ggaatgggtt gaggggcata atcactgtgg
    49981 tagccagggg aaagcgttaa gtcccgaagg gtgacatcag gttccgggca gtgagtgcca
    50041 ccgggagtga atgggagttg ttgcttttgg gagaaaaatg tattgtgaat gcgggtatac
    50101 agctggaaca gaaaaaaata agatgcccgt ggttcttttt ttaaccactg tgtatattcc
    50161 tctatggcac cgagctgttg tttgtgccgt tttacagcat cgatatacgg acagctggtg
    50221 cagatatcat ccagttcctt tcccacaata cacctcacct gcttttctca catttgcaga
    50281 ttttaaaaaa gcaaacaaaa acctccgtac atgatgtaca gaacaatcta ccaggtagtg
    50341 gcgtcagaaa acctccgggc cgcctgcact ttccggaatg cgccggttat gaaaaaccgg
    50401 aaattcagtc tctgttgtaa gatgtaccct ctgatactac gttttctctg tgtatttaac
    50461 tcactgtatt tattgtcttt ttaagcttga ctgatattct ccggtcatag tgtgccggat
    50521 acacatcccg taaacttctc atttttatat aaaaatacct gcactctctt cagcgcatct
    50581 gtctccttta agtaaaaaat gtttttatgt attgctgtaa attcatttta aatcagtata
    50641 ttaccttgat attgctgtct cctggtttga tgcgtaatcg ttctgccttt ctggatgatt
    50701 ctggtgcttc cgggtgcatt ctcctctgca catgcaacag ttcgtcatcg ccacgctccc
    50761 aaaaagcata taaaaacgaa tcgaatttga tatttttttc aatcgttttt cataggaatt
    50821 tcgtagggta ttctccgcta taacctccat atttgctgtc tttttcttca taaatcgtat
    50881 gcaatgtagc ctgtctgtca agacctcttc tctgctgttg aggagacgat ctcatttttg
    50941 gtcttttccg ggagagaggc tgtcagatga gcgagccgga tatactgtca gaggtacacc
    51001 ccgtagcaga cctgtgctcc catttccgcg atccggaacc aacaacaccc tacggttaat
    51061 accatttgaa gcgtcaggta ctgcaggaag ttcccccctg cgcggacatc ggttcctgcc
    51121 gacatctcac aatacggcca gactgtcatg cagtaacacc cgcatgcggt ctgcggtttt
    51181 aagcgcactc tcccgttttc agtgcaccga taatgcgtca tagtaagtct gggggaagtt
    51241 acggaatgcc tgttaagtgg tgccgtgggt ggagacagct tacttttcta cagcaactgc
    51301 agtcattcag gatgatcagg agatattctg tcaatgtcac gatcctgcca ttttcgtacc
    51361 cgctaagata cttccgttac tgatgctgtc cgcttttcac catttcatcc tcactgacag
    51421 gcgttgcgtt ttgggtcatc accacagctt tacgtttctc ttttaaccgg taacgctctc
    51481 ctttgatatt cagcgtggtt gagtgatgca gcagtcaatc caggagcgct gttgtccggc
    51541 aaccaccata tcaacttctt cccgggtgtg gcggagaggt ggtatcctgg ctgagtttcg
    51601 aagcgaaccg ttctttttga cgggcgcatt ttacgtccgg gctgaatgta gtaacgcggc
    51661 atggaacgtc ggcccgtata ccattgcctt aatctccgca aagataacct cgtcattccg
    51721 gacattctct gccaggcata tgtcgatgta atccataaac gatttcagct tacccctttt
    51781 gttgtcacga acggtgcaat agtgatccac acccaacgcc tgaaatcaga tccagggggt
    51841 aatctgctct cctgattcag gagagcttat ggtcactttt gagacagtta tggaaattaa
    51901 aatcctgcac aagcagggaa tgagtagccg ggcgattgcc agagaactgg ggatctcccg
    51961 caataccgtt aaacgttatt tgcaggcaaa atctgagccg ccaaaatata cgccgcgacc
    52021 tgctgttgct tcactcctgg atgaataccg ggattatatt cgtcaacgca tcgccgatgc
    52081 tcatccttac aaaatcccgg caacggtaat cgctcgcgag atcagagacc agggatatcg
    52141 tggcggaatg accattctca gggcattcat tcgttctctc tcggttcctc aggagcagga
    52201 gcctgccgtt cggttcgaaa ctgaacccgg acgacagatg caggttgact ggggcactat
    52261 gcgtaatggt cgctcaccgc ttcacgtgtt cgttgctgtt ctcggataca gccgaatgct
    52321 gtacatcgaa ttcactgaca atatgcgtta tgacacgctg gagacctgcc atcgtaatgc
    52381 gttccgcttc tttggtggtg tgccgcgcga agtgttgtat gacaatatga aaactgtggt
    52441 tctgcaacgt gacgcatatc agaccggtca gcaccggttc catccttcgc tgtggcagtt
    52501 cggcaaggag atgggcttct ctccccgact gtgtcgcccc ttcagggcac agactaaagg
    52561 taaggtggaa cggatggtgc agtacacccg taacagtttt tacatcccac taatgactcg
    52621 cctgcgcccg atggggatca ctgtcgatgt tgaaacagcc aaccgccacg gtctgcgctg
    52681 gctgcacgat gtcgctaacc aacgaaagca tgaaacaatc caggcacgtc cctgcgatcg
    52741 ctggctcgaa gagcagcagt ccatgctggc actgcctccg gagaaaaaag agtatgacgt
    52801 gcatcttgat gaaaatctgg tgaacttcga caaacacccc ctgcatcatc cactctccat
    52861 ctacgactca ttctgcagag gagtggcgtg atgatggaac tgcaacatca acgactgatg
    52921 gtgctcgccg ggcagttgca actggaaagc cttataagcg cagcgcctgc gctgtcacaa
    52981 caggcagtag accaggaatg gagttatatg gacttcctgg agcatctgct tcatgaagaa
    53041 aaactggcac gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccggcg
    53101 gtgaaaacgt tcgaagagta tgacttcaca ttcgccaccg gagcaccgca gaagcaactc
    53161 cagtcgttac gctcactcag cttcatagaa cgtaatgaaa atatcgtatt actggggcca
    53221 tcaggtgtgg ggaaaaccca tctggcaata gcgatgggct atgaagcagt ccgtgcaggt
    53281 atcaaagttc gcttcacaac agcagcagat ctgttacttc agttatctac ggcacaacgt
    53341 cagggccgtt ataaaacgac gcttcagcgt ggagtaatgg ccccccgcct gctcatcatt
    53401 gatgaaatag gctatctgcc gttcagtcag gaagaagcaa agctgttctt ccaggtcatc
    53461 gctaaacgtt acgaaaagag cgcaatgatc ctgacatcca atctgccgtt cgggcagtgg
    53521 gatcaaacgt tcgccggtga tgcagcactg acctcagcga tgctggaccg tatcttacac
    53581 cactcacatg tcgttcaaat caaaggagaa agctatcgac tcagacagaa acgaaaggcc
    53641 ggggttatag cagaagctaa tcctgagtaa aacggtggat caatattggg ccgttggtgg
    53701 agatataagt ggatcacttt tcatccgtcg ttgacatttg tggccagtct ttctggctgg
    53761 cggttcaggg gatgttttct ggttatcgtg taccacaaag cagggatatt gccccgatag
    53821 tcacatttca gattgatcgc ggtctgaaga tgtggcactg aggtaacgtg ctggtgtcac
    53881 tggtattgct gaagttatgg tttttcgggg tcaggtgagc ctgttgtgtg tatgcttttt
    53941 gttcaggtca gagggggact gtgtggacag cggcttttgt ctccgggcat tcacgcctga
    54001 acgatgaatg cgattttcag caatatttgt ctttgtgaca ctctctggtt gagaagctaa
    54061 tgcgcgcccc tgtttccaca gagaatgagg cttgccatca gccgaaacat cagtattcca
    54121 cgccatacag acggcgcctg accatgtgct gatgcaggta aaatgtttca ctacaactgt
    54181 tcgggttgct atgcaacgaa acagcgattc tcacgatctt taactgcaac gtttttccgc
    54241 ctgaaagctg actgcctcct gctgaggtgg gaggctatga ttcagtgcgt ttatagtctc
    54301 cggggtatgc atgtaccgga gcataccatt tctggtggat aacaagccgg cacattcaga
    54361 ctgcagtgaa gatggaggca cctggttgtg aagaacgttt ctgcccgtgg ctgcttcgca
    54421 tcatcaatgg tattcatacc cgctcagaaa tgaccgggtg ctgaagccct ccgttatcgc
    54481 gcagtataat gacgtatatg atctgtggac gagtgagcgg cgtgcctgat gtacgccgct
    54541 attactgtat tttaccacag ggctggtttt ctgctgccag ccctgtggtt ctcagacatc
    54601 aactgtgagc ggcatcaacc gatgtacggt atgccataag cacgtactga tccatcagat
    54661 ttgtcagtgg ttcactctga ccctgacgtg caatgatcca ttgctccaga tctttttctg
    54721 atttgtgttt caggtttttc gtccagtcgt caaggctgtt tccggtcatt aagtcaagcc
    54781 agcagagcca gaccagtttg agacgtaact ccgtgccgcc aaactgacgg aagaggctga
    54841 tcagtttgtt tgccagagaa tgttgcttca ggcgctcaca ttcagacagc aggatccctt
    54901 ccagcatcgg atgatgaacc agcaggtgtt cgctggtgca taaattcacc accagatcat
    54961 catccaggga cggggagctc cgtttgtccg gaccataaat cagccggtgc agactctggg
    55021 gaacgatggc aatctccggt ttttcgccat tgaatgacac ccacccgggc gtcagattat
    55081 ggaaatagta gatattcgga tgacacacgc cccatgatac ggcgtgccgg gtgaagccgg
    55141 tcagccattt ctggtagttt tcatggagca gctcgtaacg ggtctgatac tgttgtttca
    55201 ttttcatatt cctcacgggg gataacaggc agcagagcgc cgccggcagg ccggagattg
    55261 cgtctgctgt gggcgaaggg ttagtggtta aaaattacgg acgggactgt gcaatacgcg
    55321 cctgcagcca ggcttccact tcacttttca gccagcggga gctgcgcccc attttgatgg
    55381 gcgcaggaaa gcccccatct ttgatgagtt tgtcaaacca cttatcggtt aggccggtca
    55441 gttgagtgat aaacgccatg tcgaccatct ggtcatccat cagcgaaact ggggtagcca
    55501 tgagaatgtt cctccggatg ttgttaaacc agccggtgtg gtcatgaaaa cagcgcgcca
    55561 ccttcaccgg cataacaacc ggggagaaac gtaaaaaata atcgggaatg cggctgtccg
    55621 gagataaacg gggggattgc gatggcagag ccgtagcgga atcccggagg ggggtgttgc
    55681 gaaaagatgc ttccgcacgc acgcgcgata aagaggggat agttttcgtg aggacaggga
    55741 gagaagcagg cgttctgata attgcagagt tgttttcgga ggtggttaca gtgagtcagc
    55801 ccgttcctgg cgagcaggag actgatatca ccgatgggat aaggttgtct gattggtatt
    55861 ataaatataa gaagtcatca gagagtaacg taaaggattt gccagaataa cctaagagag
    55921 taaatctctc cggtaacaga gcgctgtgaa gcattctgta tcagagtgca gatacatgaa
    55981 cgctgatgcg taaaatcagc agtgacacgc cagctccccc tatgcccatt cctgtataag
    56041 acattaactg agtgtattaa tgcttctgcc ctgagactga gccggtgagt gaggggctga
    56101 gaaatccctc ttgacacagc tctaaccaga aatacttccc cggttgttgc ggtgcgtccg
    56161 gatccccgat aacggaacac taaggctgta ttgcgggaca ccgggtaagt agcagcccgt
    56221 aaggcgttct gtcaacgcca gaaaaaatga tttcccacac gcgttataga ctcactcctg
    56281 tggacctgga agcccgtaac tctgcggttg cccactgact gacggaactg accgcaaatt
    56341 ttacgagtgc gtgctgggct tcagtctggt cacctgagcg ttcggtaacg gaggcgcggg
    56401 cggtaatagg ctgattttca ggtattaccg gcttacagcg tgtggttgat attgaggggc
    56461 tgtgagtttc gcgtatttgt cagaaaccca gacgagtttt cctgtatggg acgaaataag
    56521 gcagcggcaa tacctggaaa aatttaatat ggtatgaagg agaacctgtc ggttacgctt
    56581 aatatacccg gtacactgtc agctcagggg atacatgaac cgttatctca ccagctacac
    56641 agtatataca cactgacgct gacagtagtc agcaggacca caataatcag tgccagcagt
    56701 atggtaacga tacggtctgt attccggtaa tagccgaagg ggtcgacaga accacaatac
    56761 gggcaacaca ctgtattatg agtaatcgcg tttccacagt ccctgcactt ctttttgcgt
    56821 atcaccatca ctccctcctc acagccttac ccgtaaccgg ttttttacat taaaaaaaca
    56881 acgctgaaaa ttcaaccgtc agtccggaga cgaccgttcg ggttatcaca gagtgcctga
    56941 gacagtgtcc tgccggaggt cacttcaggg actctgcgca ttttttacga cgtggctatt
    57001 ccgtatgaac catacggaga tttaaccatg acctacaaat acaacccctt ctggcagcaa
    57061 cgtattcgtg agacggtgcg gcacgcactg aatgttcatc cccgcctgac ggcattgcgg
    57121 gttgacctgc gtttcccgga tgtaccggca gcaacggacg cagctgtgat atcccgcttc
    57181 atcaatgccc tgaaagcccg aatcgacgct taccagaaac gtaagcatcg ggaaggtaaa
    57241 cgcgtgcatc ccacaaccct gcattacgtc tgggcccggg agtttgggga gtgcaaaggt
    57301 aaaaaacact atcacctgat gctgctggtt aaccgggata cctggtgtcg tgccggtgat
    57361 taccgtgctc cgggatcact ggccgggatg attaaacagg cgtggtgcag tgccctggga
    57421 gtggatgtcg ggtgccatgc cacgctggtg cattttccgg cctggccggc ggtgtggctg
    57481 gaacgcgatg atgacactgg ctttcagcag gtgctggaac gtgctgacta tctggcgaag
    57541 gaacatacca aagctcactg caccggtgag cgcaattttg gctgtagccg gagttgagca
    57601 cgactggcgc gccaataccc ggcaaaccaa aagtatgggt tgttgtctgg cggcaacgat
    57661 gtatccactc gcccactgac tgttttttac gtcagtgttc cattgtgtga caaccacagt
    57721 gctgtaacag caaccagcct gctttaccca aactccagtc cagattttaa ctgaaccgcc
    57781 attcacaccc tgaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctggcg
    57841 gctgaggaat atgactatgt acgcaaaatc ctttatcgct cttgatggca acggacgtct
    57901 gacgggcgcc cgtactgcac aggccgcacc ttatgctaac tacacctgcc acttgtgtgg
    57961 cagtgcactc agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga
    58021 cgacaggctg acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat
    58081 acagttgatt aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc
    58141 cagctggcac tgcagacaat gtcaccacga ttattatggg gagcggtact gcacacactg
    58201 ccagaccgga ggattcagta ttccccggac aactcaggag gaaatatgcg aattctgaac
    58261 tgttatatgg cgaatgacag caaaggccat tttgttacgg cgaaagaagc agcgaagcac
    58321 aaccgccagg acgttttgtg ctgtgtgtcc tgtggatgcc cgttaacact tcagcggggc
    58381 aatgacggac aaccaccgtg gtttgaacat gatcagatga ctgtcgctga gaaaatcctg
    58441 ctgcgatgca cctggcttga cccggcagag aaagaggccc gtcgtttgca tctgcagggt
    58501 atgacggttc cggattatac ggtgaaggtg agaaagtggt tttgtgtgat gtgtgacgaa
    58561 aattatgagg gggaaaagtg ctgcccgagt tgtggtaccg gggtatacag cagggagggg
    58621 gggcaggagg gcaactggaa ggataggaac tgagcggttc agtcgcggaa atcagttgca
    58681 gagctgtaat gatatctccg ggagtgctgc gttggtaggg gcatcccggg atagagaaat
    58741 tttagcactt tgccctgaat gattgtagac gtcccgagta ctcgtaccgg tctgagttat
    58801 tacagttaga tattcacttt ttctcccgca tacttcacat ctgtcaacga cggatgaaaa
    58861 gtggttcttc cgcaacctgg gtggaagaaa atggatgaaa ctttctggcg aacaaggatc
    58921 taagctgttt taagttatgg gcaacgcaat gcactctctt aagacacttc tacagttacc
    58981 ttgcggatgg cgatgcagtc gacaaattat tagctctgac ggtataaccc tccatctcca
    59041 cggaaaacgc aaaacagcac aatgtcctga atgctttaag cgtagcgact ctgttcatag
    59101 ttgtcgtcgg cgccggatac agcatctacc ctgctcaggg cagacgctat ggcttgtatt
    59161 ttccgtccgc cactggtact gccgtaaccc ggcttgttca cgtaaaattt ttgccgagtc
    59221 gcttgctccc ttcgccagtt cacaccagca gtcttcacag gcgttacaaa attaacaacg
    59281 tcaactggga ttaatagctg gaggtgaggc tggaaaacgg gctgcaacgg cagtgggtct
    59341 ccgttgcagt gctgatactc ttcttcgcag gcttatcaat accccggaga cgaaacagtc
    59401 aggcgcgcct catgtcggta ttgatgagtg ggcgtggcat cggggccact gctgcggtat
    59461 gttaatagtc aatcctgata ctcaccgtcc cctcgtcctg cttcccggcc gtgatcagcg
    59521 tacgctggcg acctggttca gaaaatatcc ggaaatacag gttgtctcgc gtgatcgcag
    59581 tggagtctat gcgacagcag cacgtgaagg tgcacctcag gccagacagg tggccgatcg
    59641 atggcacctg ctaaaaaata ttggtgatga gcctgaacga atgatgtaca gatatatgcc
    59701 tctgatacgt cttgttgtcc gagagttatc actgaagaaa tcacctgagc cagaaatatc
    59761 tgtgcctgta gcatcgctcc gtcgtctgga acgccttaaa cagcacatcc gcaaaaaacg
    59821 gcatcagcgt tggacagagg ttatggccct gcataacaag ggatgtagtt tcagggaaat
    59881 atcccgtatt acaggcctgt cgcgagtgac agtcagtcgc tgggtgggtt caggaacatt
    59941 ccctgaaatg tcaactaggc ctccaaagcg agggcttctg gacccatgga gggagtggtt
    60001 aaaagagcaa cgagaatgtg gtaattataa ctccggccgg atatggcggg aaatggtggc
    60061 caggggggtt acaggcagtg aaaccatcgt cagggatgct gttgccaaat ggcataaagg
    60121 ctggatccca ccggttacta ctgccgcaag acttccttca gtgtcccggg taagccgctg
    60181 gttgatgccc tggagaataa tcaggggtga agaaaattat gctttccgat ttattagtct
    60241 gatgtgtgaa aaagaaccgg agttgaaaat agcgcagcaa ctggtactcg agttctaccg
    60301 tattctgaaa acctaaaata aatcacagct aagcagctgg ttcactcggg tccacgaaag
    60361 cggctcagca gaacttcggt gcgtagctgc ggggatggaa gctgatgctg cggctatatg
    60421 tgaggcaatc agcagtcgct ggagtactgg tgttgtcgaa ggtcatgtaa atcgcctgaa
    60481 ggtgctgata cgccagatgt atggtcgagc cggacttgaa ctacttaggc ggagggtaat
    60541 gagtccactg gcatgaaaat cttcacctag gttgaggaag agccactttt gcactgttcg
    60601 tgacagtatg gcctttatgt actcactttg ttaccggtag ctggatcatc actaccgtgc
    60661 cgcaaatggt ggtgaacagc atcaattgtg gaatgaatgt tgccgaagcg accaatgcgc
    60721 ctcgtttcca ccatcagtgg ttgccggacg agctgcgtgt tgaaaagggt ttagtccgga
    60781 tacgctcaag ctgctggaag caaaaggtca gaaagtggcg ctgaatgagg cgatgggcag
    60841 cacgcaaagc attatggttg ggtcggatgg tgagttgtac ggcgcatccg actcgcgctt
    60901 ggtggatgat ttaacggcgg ggtattaatt gacatccccc ggcgcaatgt gccgggggat
    60961 aatggtttta tgcggtcaat aatcgtccgg gattatcgat acagagtttt ttgaggatcg
    61021 ttttatcgac gccgcgctcc gccagatagg ccagaaaaac atctggaaca aatccccagc
    61081 cattaccgcc atttttagcc cacatttgtt taaggaagac atcgtggctg agaacaagct
    61141 gatcggcgta tcccaactca atgagatgag cgacggcatc ggcagtctcc tgcacccctg
    61201 gcgctattcc ctctttcggg aaggtaatgt ctaggccaat catgtcgaat tccagccaga
    61261 cacctttatc aagcattttc cgctgatacg ccacgtcttt tcctgacgga tccgagtgtg
    61321 cgagagagac tttatttggc gagacgccca tttcacctaa cacaatgtcg agtacttcat
    61381 caccgcgacg aagccagccc ggcatgtgaa tattcatcgc cacatgagga ttgttaatct
    61441 gtgccagcga ggcagcccgc aagctgttat gctcggcttc ggtaaatgtc ggtgagacac
    61501 ctatttcacc gatcattccg gcacgaatat ccgtatcgcc aatcccttga ttcaattctt
    61561 tatcgatcgt tgtcgctagt tcatcaaccg ttttatgaat tctctggctt tcaaattttt
    61621 ccaggtaggg accggaggaa gcaacaatat tcagacctgt ttttaatgcg acttctcgta
    61681 atgcctgcgc atctcgtccg atagactcag accctgtggc atcaacaatg gtgcgaccac
    61741 cgagcgagat aaagttatta atttcgaaga tcacatcttc aataggtttg cggtccatat
    61801 tatcagcgca gcaataagga tcgtgtttta atgcccactg gatttctgcc gtgacttttt
    61861 tatcaacaag ctgttgtgaa aaaggatagc aaggagcatc cacaacagag gaaagatcgt
    61921 tgaacagatg ttcatgagga agtgttagcc ccatatcttc acgggcgacg gggcccgtca
    61981 cagtttgtag ataatctttc atgattatac ctccattttc ttcattgttt tcgaggctct
    62041 gtattccgga atagtggtaa caattgcacc gaccagtgca aacagtgttc cgatgatcgt
    62101 gaccagataa acggtattgc ccagcgatgg aattaatacg tcaatcaata ctgatcccag
    62161 taactggccc gccgttgaag caacccccag catcagtaac cccagacccc tgaccaaaat
    62221 ggccattaaa ccgatagaca ttagtccaag cgggccgcca agatacatcc accagacatc
    62281 tggtaattga acagtgacgt gccctaatgc gacacgaata gctagcgctg tgcctaaaac
    62341 acaaaatcca acgatgaaat tccaggtgat tgaaaccagc atggatcccg tagcttcagc
    62401 tacttttgca tttccagcgg gctgccatcc tgcaagcaaa ccagctaaga acggaagaat
    62461 ggcaagtaaa atgaaagagg tagaatgcca ctgcggtgac acaacaaaaa tcgtagcaat
    62521 aaccgcaaat aatgcaccga tgatgcgata aggggtaaag tattttttct cgtcgacacc
    62581 gataccaaag cgatcgcaaa gtaaaccaga cagcaatagc gcggagatca gtgcggtctg
    62641 aaacgttgcg accccgagtg cgctggctga tgcgccttca gaaaatacca ccatcgctcc
    62701 acagagaccg gcaaaccagt tccagagagg aacttttcgg tttttaatca gagaaggaat
    62761 tgccgcaaat tgttgccgga tttcttttcg cgccataata ataaaaaaca tcacgactaa
    62821 accactggca aaggagataa ctgcgcatgc attaccgtcc tgtaaccagt gacctaattg
    62881 accattcacc gctgattgca taggggagag catacccgca agaatcgtgg caagcaccca
    62941 taagggagtt gagtacttat tctttttcat acatcaatcc ttctattata gtaaatggag
    63001 taactggtgt taccccatta ttattattat cttgcaatat gagtcagtat atttacccac
    63061 aacgcggtat agaacggcca ggacataaac tgctgtgtac cccagtgcgg tgaacagtca
    63121 ctcataaagc aggctatttt cccgttgtga tagttaccaa agaccagtaa tggatcgttg
    63181 tttattgtta ataccacctc agcattttct ttagcgatcg cccggttata acctaggaaa
    63241 aaaggatatt cagagaaccc tttaataacc ggatggtcag gttgtgatgg ctgtgcaata
    63301 acaccctctg gtttttcgac acgatcgtca ccatctaaca tcgtaaccgg gagcacatca
    63361 gccagaactg tatttttata attggctttt gcttcaattc ccatgaatga gagatagccg
    63421 ccaatcatca ataacccacc accgttattc acatattatt taatcagttc gagagcgttt
    63481 ggttttatgc gaagctgata aaaagtatca ttttgtagca ggaaagtatt actaccaata
    63541 tcactaataa cgatggcatc atattgttct aactgtgcca catcttcagg gaaggcaatt
    63601 tgtaccgtat gtgccggcat ataggtgaca tctacctgac tatttttcag acattggagt
    63661 aaccaggtcg caccttcttc atatttactt gaggtaaaac tgtcatatcc ttttgaatga
    63721 atcatatgaa tatgccagga ttctccaata aagagcacct ttagtgattt tttggtgtta
    63781 ttcattctct gtcccttctt aatgcgtaaa aatagtttgc tgataatgta tactctgaat
    63841 tcgatgtatc acagattcat gttcaggcat gtcggaagca ttgcttgtct ccaccgccag
    63901 ggatgcaaag gcgctggcat aacacagtgc tgattccagt gattttcctt tcgcaagtcc
    63961 tgatgccaga gcgccgttaa aagcatcccc ggctcctgcc gtatttttaa cgaccgctgg
    64021 aaacgctggc gaatgaatga attttttgcc atcaaatgcg agcgagccct tgctaccgag
    64081 cgtgataacg gtgtttttta cccctttatt gtggatagct tctgcagctc gtttagctga
    64141 ttcaagatcg tgtacgtcta tgccggacag taaaccagct tctgtttcat tgggggtgat
    64201 ataatcaaca tcctgaataa gttcgttgac gatatcatta tagggtgccg ggttaataat
    64261 gaccgggata ctgttttttt gcgccaatgt gatcgcttgt tgcagagcgg tataatttgt
    64321 ttcgagctgc agaagtatga catcagaatt aataatcgct tctttctgaa tagttatttc
    64381 atcggacgac attgtcatgt tggcaccggg atagatcgca ataacattat cgccagtacc
    64441 ttcattaaca aggatcgtgg cagttccggt ttgcgtttct ttcgtttgat aaattatact
    64501 tttataaatc ttagacgaat tcataaagtt gatcgcataa tcactaaagt gatctgtacc
    64561 tacttttgtt atgaaataaa catcagtatc ggcaaagctt gctgccagcg cctggttaca
    64621 accttttccc cccggtgaaa atataaaatt acttgccaga agcgattctc cgatagtggg
    64681 aagcctcggc aaataactga tgatatcaac attaaacgaa ccaataacgc atacttgact
    64741 tttcatagtt gagtggcctt tttttattcc ctaggctgta tcataatttt caagcgcata
    64801 agcgatttca tttttcgcaa tagtatcaag cgagtctagg acgataaaag caccgccatg
    64861 atatcgagtc agatagccaa gtctggcaag ttcattaaga tctgatcgaa tagtttcttt
    64921 cgttacattg aggctattgg acagatcgga tacttttaca gaaccgaatt tactgagtcg
    64981 tttcaatatt tcatgatggc gttcaaattt catgcttcac ctcacttcag ttacgttaat
    65041 atctgtcgat gtatattgat gtgataatca ccataaagtg gcggggaaaa gcaaaaaaaa
    65101 tcaaaaatag ccggagaaaa ggtaagcgtt aatggagaat catattgatg cttatttatt
    65161 ggtgagaact acgttccatg gcaggagttc atcaatacgg ttggaaggtt attccggcag
    65221 tacgctcagg atatggcgca gatactcttc cggatcaatg tcgttcagaa ggcaagtgcc
    65281 gataagccca tacaacagtg cactgcgctc gccgtcgaag aacataaagt ttttatttcc
    65341 gagacagatg gcacaaaggg ctttttccgc ggcattatgt ctgcctccgc ctgaccgtca
    65401 acactgtaat aacagagtgc gccccactga ttcagtacac agctgaacca ttctcccagt
    65461 ctggattttt ggacagcgtg gcatttttct ccaccatcca cgcatgcagt gagtccagta
    65521 acagtttgct cttcctctgc ctgaccacaa gatgttctgg ttctggcata ctgaatttgg
    65581 ccacctgagc ataggtaata tgctcacctt agaacaacac agatgctcca atgaaaaaag
    65641 gaaatttcag cgcagagttt aaacgcgaat ccactcaact agttgttaac cagaacttta
    65701 cggtggcagg taccatccaa agctatggat gtcggtcttt ccacaatgac aagataagtc
    65761 aaacaactgc gtaatgagca tcagagcaaa acaccaaaag cctctctgat gacaccagaa
    65821 caaatcgaaa tacgtgagct gaggaaaaag cggcaacgta ttgaaataga gaatgaaata
    65881 ttagaaaagg ctacggcgct cttgatgtca gactccctaa acagttctcg ataatcgaga
    65941 agctcagagc gcattatctg gtgatagcac tctgccatgt gtgcaggatt catcgaagca
    66001 gctacagaga ttggaaaaac cgtcctgaaa aaaagacggt agacgggctg tattacgtag
    66061 tcaggtaatt gagcttaatg gcggagcaag gaacatcgcc acaattgcaa cccggagaga
    66121 ctaccagatg ggacgcaagc ttgctggcag tctcatgaaa gaacaggggc tggtcagctg
    66181 tcagccgttg accctccggt acaggcatgg cagtcatgaa cttgtagcta tcctaagaac
    66241 ttgaagggcg cttagctgtg acacagctaa atcaagtatg gtgacgtgac ctatatctgg
    66301 actggtaaac gatggggata cccgcttacg cgagagcaat aagtggctgg ccaccattct
    66361 cgatacaaaa ctctgctgaa tagcagcgat tttcccagcg tggtgcgtct tttgcatttt
    66421 actggttctg agttatcaat tatctgttca ctaatgacta ctctaaaaag ttgtatcaat
    66481 ttaagtgata tagggagtcc ggagattcac cctttttggt gatgaagtta acaataccgc
    66541 caattgcctg tgaaccgtac agtacggaat atggaccttt cactacctca acacgctcca
    66601 gcgcagactc atctatcaac agtcccacac cataattatc tccggcgcgc tgataagtta
    66661 cctcctgacc atcaatgaga attaaaacac gggaggatgc ttcgccacga atgcggattt
    66721 gtttacggcc tgccaaggag ttgtctgtta tctctactcc ggggatgtcc tgcaaattat
    66781 cggctatgga ggcattggtg gagttctgca atgtttgttg gtcaataacc tgtatagtcg
    66841 ccgggctttc ccaaaggttg cttgccgaac gattcgtggt cacgacgagc gtctcttcgt
    66901 tgctgatttt tgtttctgaa gaagttgcgg ttgcagtaga aactatccca gaaatgcata
    66961 atgacagagt ggaaaggata taaattcgac aaggtatatt atataacatt tcatccctca
    67021 ttaatacaaa gtacaaatga ttgctgtaat gataattatt ttcatttatg ttctcatata
    67081 tctataatta ttgtcatctt cataaattgt aaatatcaaa catttttgac cttaatcatg
    67141 ttttttgata gatattttac gactgaacat atccgtcgtt tcctctgact cgtgtcagtc
    67201 ggtctctggt tgtatccacg gaccgcactc ttgacataag agtgcggtcc gtggaaaacg
    67261 gagttgaaaa aatggtgtct gaaagaaggt accatcattg tgatgacaaa tactttggcg
    67321 tggataatgt aattaattgc tgaaattgac cgtatatgcg gtaagactca tatcagtatc
    67381 aggtccggtt aattgctgat actaatataa actttttaca aaaggatgaa tgcagaaggt
    67441 tactctaatg gccagagtga tgacaatgat ggtcagacgt tgacctgcag aaactgcatt
    67501 ttaattggtc tataaagtct gcaagaggaa tcagggtcag atcagtgagt cttatcgcgg
    67561 cccgaagcta tgactgaaag aaggctggat acagaattgc aacaatttgg caatgtcaga
    67621 acacagaagc tttgcaagca ggtgaaaggt tatgtctagc aaaaggagta tatcagaata
    67681 tatctactga tgtacaaaac acaacaatag cttcatgata tataaatacg tgatgacgag
    67741 tggtactccg catggttatt taacttctca gcaccactaa tgatcctgag caacttcgta
    67801 tttgggccat taccatggag tagaaagctg tgtctgagaa tgcagaattt gagaaaagaa
    67861 ttcgctttct tggagagctt ctgaaaatgg cacgctaaca acgttttgtt aaaaaatgtg
    67921 aatcacttgc cggggctgca ttttctattc gaggagaatg ttgacgtaga tattacagaa
    67981 atctcagcgc atcaggataa actgctacct taggtcaacc accaaaagaa aatacaatac
    68041 gtccagtttg tatgccttta ataacacatc ttctaaatgt tgaagaatta tcccgcctgc
    68101 tgaagaacgt tgctctgagt gtgataaacc tttttattct tgatgatgtc agcgagaaga
    68161 tggaatatat ccccgactgg gtcgtggtta cccggttaaa catctggtgg cgctaataaa
    68221 tctggcaagt cacattctgt tgaatgctgt gacagtgcct taccgagaga gcgaaattgt
    68281 ggtgttctag tgcatagtcg ccaccattcc ggataattct attacgttgt tttacaagtt
    68341 cttttacagc gcagatctgt cgctgacgtt gaatcaatag cctggatttc agaacacaat
    68401 gcccgtttgc tggttgttcc ggcagtagca ttaattcatt atctgaatga aatgtgtcaa
    68461 tccacagaaa ccgctgtcaa tgacgcattt tgtcggacgt taacacgaga aagtctggct
    68521 attccgtaat gccatcttat ttcgtaatgg attgagaatg ctggattttt aattatatta
    68581 cgcgcagtat taatcgcgta aatgttttgc ggtcagcaac ggtgggggat ccattgttct
    68641 gttcaatgaa taccatcata catttgaatc aggagtcggt atgatgattt tggtgacggg
    68701 gggcgcacgg agcgggaaga gtcgccacgc agaggcgctt attggggact cttcacaggt
    68761 tctgtatatc gctacctcgc aaatccttga tgatgagatg gctgcacgga tagaacatca
    68821 tcggcaaggc cgcccggagc actggcgcac agtggagcgc tggcaacatc ttgatgaatt
    68881 aattcatgca gacattaacc cgaatgaggt tgtgttgctt gaatgcgtta ccacaatggt
    68941 gactaatctg ttgtttgatt atggcggcga taaagaccct gatgaatggg attatcaggc
    69001 gatggaacag gcgattaatg ctgagattca gtcgttgatt gctgcctgcc aacgttgtcc
    69061 cgcaaaggtt gtattagtga ctaacgaagt gggaatgggg attgtgccgg agagtcgtct
    69121 ggcacgacat tttcgtgata ttgccgggcg ggtaaatcag cagctggccg ctgc
//
LOCUS       DAFATA010000012        65365 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFATA010000012 DAFATA010000000
VERSION     DAFATA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.012"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            84..497
                     /locus_tag="LGJ20_001133"
     CDS             84..497
                     /locus_tag="LGJ20_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK2385230.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(542..859)
                     /gene="hspQ"
                     /locus_tag="LGJ20_001134"
     CDS             complement(542..859)
                     /gene="hspQ"
                     /locus_tag="LGJ20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBK2385231.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(917..2107)
                     /gene="rlmI"
                     /locus_tag="LGJ20_001135"
     CDS             complement(917..2107)
                     /gene="rlmI"
                     /locus_tag="LGJ20_001135"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBK2385232.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2202..2480
                     /gene="yccX"
                     /locus_tag="LGJ20_001136"
     CDS             2202..2480
                     /gene="yccX"
                     /locus_tag="LGJ20_001136"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK2385233.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(2477..2806)
                     /gene="tusE"
                     /locus_tag="LGJ20_001137"
     CDS             complement(2477..2806)
                     /gene="tusE"
                     /locus_tag="LGJ20_001137"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBK2385234.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2897..3556)
                     /gene="yccA"
                     /locus_tag="LGJ20_001138"
     CDS             complement(2897..3556)
                     /gene="yccA"
                     /locus_tag="LGJ20_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBK2385235.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3763..3850)
                     /locus_tag="LGJ20_001139"
     tRNA            complement(3763..3850)
                     /locus_tag="LGJ20_001139"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3814..3816),aa:Ser,seq:tga)
     gene            4277..5395
                     /gene="hyaA"
                     /locus_tag="LGJ20_001140"
     CDS             4277..5395
                     /gene="hyaA"
                     /locus_tag="LGJ20_001140"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBK2385236.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5392..7185
                     /gene="hyaB"
                     /locus_tag="LGJ20_001141"
     CDS             5392..7185
                     /gene="hyaB"
                     /locus_tag="LGJ20_001141"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBK2385237.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7204..7911
                     /gene="hyaC"
                     /locus_tag="LGJ20_001142"
     CDS             7204..7911
                     /gene="hyaC"
                     /locus_tag="LGJ20_001142"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBK2385238.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7908..8495
                     /locus_tag="LGJ20_001143"
     CDS             7908..8495
                     /locus_tag="LGJ20_001143"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBK2385239.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            8492..8890
                     /gene="hyaE"
                     /locus_tag="LGJ20_001144"
     CDS             8492..8890
                     /gene="hyaE"
                     /locus_tag="LGJ20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBK2385240.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVESQQERAS"
     gene            8887..9744
                     /locus_tag="LGJ20_001145"
     CDS             8887..9744
                     /locus_tag="LGJ20_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBK2385241.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            9878..11422
                     /gene="appC"
                     /locus_tag="LGJ20_001146"
     CDS             9878..11422
                     /gene="appC"
                     /locus_tag="LGJ20_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBK2385242.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            11434..12570
                     /gene="appB"
                     /locus_tag="LGJ20_001147"
     CDS             11434..12570
                     /gene="appB"
                     /locus_tag="LGJ20_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBK2385243.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12583..12675
                     /gene="cbdX"
                     /locus_tag="LGJ20_001148"
     CDS             12583..12675
                     /gene="cbdX"
                     /locus_tag="LGJ20_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBK2385244.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12755..14053
                     /gene="appA"
                     /locus_tag="LGJ20_001149"
     CDS             12755..14053
                     /gene="appA"
                     /locus_tag="LGJ20_001149"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBK2385245.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(14168..16348)
                     /gene="etk"
                     /locus_tag="LGJ20_001150"
     CDS             complement(14168..16348)
                     /gene="etk"
                     /locus_tag="LGJ20_001150"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBK2385246.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(16368..16814)
                     /gene="etp"
                     /locus_tag="LGJ20_001151"
                     /gene_synonym="yccY"
     CDS             complement(16368..16814)
                     /gene="etp"
                     /locus_tag="LGJ20_001151"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBK2385247.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(16802..17941)
                     /locus_tag="LGJ20_001152"
     CDS             complement(16802..17941)
                     /locus_tag="LGJ20_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBK2385248.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(17987..20083)
                     /locus_tag="LGJ20_001153"
     CDS             complement(17987..20083)
                     /locus_tag="LGJ20_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBK2385249.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(20083..20829)
                     /locus_tag="LGJ20_001154"
     CDS             complement(20083..20829)
                     /locus_tag="LGJ20_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBK2385250.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(20826..21470)
                     /gene="gfcB"
                     /locus_tag="LGJ20_001155"
     CDS             complement(20826..21470)
                     /gene="gfcB"
                     /locus_tag="LGJ20_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBK2385251.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(21577..21882)
                     /locus_tag="LGJ20_001156"
     CDS             complement(21577..21882)
                     /locus_tag="LGJ20_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HBK2385252.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(22324..22536)
                     /gene="cspH"
                     /locus_tag="LGJ20_001157"
     CDS             complement(22324..22536)
                     /gene="cspH"
                     /locus_tag="LGJ20_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBK2385253.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            22822..23034
                     /gene="cspG"
                     /locus_tag="LGJ20_001158"
     CDS             22822..23034
                     /gene="cspG"
                     /locus_tag="LGJ20_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBK2385254.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            23045..23233
                     /locus_tag="LGJ20_001159"
     CDS             23045..23233
                     /locus_tag="LGJ20_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK2385255.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            23208..23438
                     /gene="ymcE"
                     /locus_tag="LGJ20_001160"
     CDS             23208..23438
                     /gene="ymcE"
                     /locus_tag="LGJ20_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBK2385256.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            23428..23601
                     /locus_tag="LGJ20_001161"
     CDS             23428..23601
                     /locus_tag="LGJ20_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBK2385257.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(23649..24722)
                     /locus_tag="LGJ20_001162"
     CDS             complement(23649..24722)
                     /locus_tag="LGJ20_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK2385258.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLATNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(24794..27538)
                     /gene="torS"
                     /locus_tag="LGJ20_001163"
     CDS             complement(24794..27538)
                     /gene="torS"
                     /locus_tag="LGJ20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBK2385259.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            27621..28649
                     /gene="torT"
                     /locus_tag="LGJ20_001164"
     CDS             27621..28649
                     /gene="torT"
                     /locus_tag="LGJ20_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBK2385260.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(28622..29314)
                     /gene="torR"
                     /locus_tag="LGJ20_001165"
     CDS             complement(28622..29314)
                     /gene="torR"
                     /locus_tag="LGJ20_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBK2385261.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            29444..30616
                     /gene="torC"
                     /locus_tag="LGJ20_001166"
     CDS             29444..30616
                     /gene="torC"
                     /locus_tag="LGJ20_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBK2385262.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            30616..33162
                     /gene="torA"
                     /locus_tag="LGJ20_001167"
     CDS             30616..33162
                     /gene="torA"
                     /locus_tag="LGJ20_001167"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBK2385263.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            33159..33758
                     /gene="torD"
                     /locus_tag="LGJ20_001168"
     CDS             33159..33758
                     /gene="torD"
                     /locus_tag="LGJ20_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBK2385264.1"
                     /translation="MTTLTAQQIACIYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNALENRIAALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCHQHDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(33851..34156)
                     /gene="cbpM"
                     /locus_tag="LGJ20_001169"
     CDS             complement(33851..34156)
                     /gene="cbpM"
                     /locus_tag="LGJ20_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBK2385265.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(34156..35076)
                     /gene="cbpA"
                     /locus_tag="LGJ20_001170"
     CDS             complement(34156..35076)
                     /gene="cbpA"
                     /locus_tag="LGJ20_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBK2385266.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            35336..36595
                     /locus_tag="LGJ20_001171"
     CDS             35336..36595
                     /locus_tag="LGJ20_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBK2385267.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQNVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKVRLVLDTDPEQRV
                     NEVKNFLTALSDSICSLFIVNSNMASISLGNESFSTDEDLEYGYLMNTGNHYDVYLPP
                     ELFAQAYKLNNKEMNAQLDYLNRYAIY"
     gene            36884..38125
                     /gene="agp"
                     /locus_tag="LGJ20_001172"
     CDS             36884..38125
                     /gene="agp"
                     /locus_tag="LGJ20_001172"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBK2385268.1"
                     /translation="MNKTLIAAAVAGIVLIASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(38163..38390)
                     /locus_tag="LGJ20_001173"
     CDS             complement(38163..38390)
                     /locus_tag="LGJ20_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBK2385269.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(38411..39007)
                     /gene="wrbA"
                     /locus_tag="LGJ20_001174"
     CDS             complement(38411..39007)
                     /gene="wrbA"
                     /locus_tag="LGJ20_001174"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBK2385270.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            39380..39553
                     /locus_tag="LGJ20_001175"
     CDS             39380..39553
                     /locus_tag="LGJ20_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBK2385271.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(39636..40964)
                     /gene="rutG"
                     /locus_tag="LGJ20_001176"
     CDS             complement(39636..40964)
                     /gene="rutG"
                     /locus_tag="LGJ20_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBK2385272.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(40985..41479)
                     /gene="rutF"
                     /locus_tag="LGJ20_001177"
     CDS             complement(40985..41479)
                     /gene="rutF"
                     /locus_tag="LGJ20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBK2385273.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(41490..42080)
                     /locus_tag="LGJ20_001178"
     CDS             complement(41490..42080)
                     /locus_tag="LGJ20_001178"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBK2385274.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            complement(42090..42890)
                     /gene="rutD"
                     /locus_tag="LGJ20_001179"
     CDS             complement(42090..42890)
                     /gene="rutD"
                     /locus_tag="LGJ20_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBK2385275.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(42898..43284)
                     /gene="rutC"
                     /locus_tag="LGJ20_001180"
     CDS             complement(42898..43284)
                     /gene="rutC"
                     /locus_tag="LGJ20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBK2385276.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(43296..43988)
                     /gene="rutB"
                     /locus_tag="LGJ20_001181"
     CDS             complement(43296..43988)
                     /gene="rutB"
                     /locus_tag="LGJ20_001181"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBK2385277.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(43988..45079)
                     /gene="rutA"
                     /locus_tag="LGJ20_001182"
     CDS             complement(43988..45079)
                     /gene="rutA"
                     /locus_tag="LGJ20_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBK2385278.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            45367..46005
                     /gene="rutR"
                     /locus_tag="LGJ20_001183"
     CDS             45367..46005
                     /gene="rutR"
                     /locus_tag="LGJ20_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBK2385279.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            complement(46045..50007)
                     /gene="putA"
                     /locus_tag="LGJ20_001184"
     CDS             complement(46045..50007)
                     /gene="putA"
                     /locus_tag="LGJ20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBK2385280.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            50062..50271
                     /locus_tag="LGJ20_001185"
     CDS             50062..50271
                     /locus_tag="LGJ20_001185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385281.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            50430..51938
                     /gene="putP"
                     /locus_tag="LGJ20_001186"
     CDS             50430..51938
                     /gene="putP"
                     /locus_tag="LGJ20_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBK2385282.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            52480..53310
                     /locus_tag="LGJ20_001187"
     CDS             52480..53310
                     /locus_tag="LGJ20_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBK2385283.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            53368..54495
                     /gene="efeO"
                     /locus_tag="LGJ20_001188"
     CDS             53368..54495
                     /gene="efeO"
                     /locus_tag="LGJ20_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBK2385284.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            54501..55772
                     /gene="efeB"
                     /locus_tag="LGJ20_001189"
     CDS             54501..55772
                     /gene="efeB"
                     /locus_tag="LGJ20_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBK2385285.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            56391..57179
                     /gene="phoH"
                     /locus_tag="LGJ20_001190"
     CDS             56391..57179
                     /gene="phoH"
                     /locus_tag="LGJ20_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBK2385286.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSTLCQRTLHAYS"
     gene            complement(57229..57642)
                     /gene="pgaD"
                     /locus_tag="LGJ20_001191"
     CDS             complement(57229..57642)
                     /gene="pgaD"
                     /locus_tag="LGJ20_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HBK2385287.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(57644..58969)
                     /gene="pgaC"
                     /locus_tag="LGJ20_001192"
     CDS             complement(57644..58969)
                     /gene="pgaC"
                     /locus_tag="LGJ20_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HBK2385288.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(58962..60980)
                     /gene="pgaB"
                     /locus_tag="LGJ20_001193"
     CDS             complement(58962..60980)
                     /gene="pgaB"
                     /locus_tag="LGJ20_001193"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBK2385289.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            complement(60989..63412)
                     /gene="pgaA"
                     /locus_tag="LGJ20_001194"
     CDS             complement(60989..63412)
                     /gene="pgaA"
                     /locus_tag="LGJ20_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HBK2385290.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            63999..65357
                     /locus_tag="LGJ20_001195"
     CDS             63999..65357
                     /locus_tag="LGJ20_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2385291.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENTLNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT        15930 a        16390 c        16937 g        16108 t
ORIGIN      
        1 ttttgctctc attcattcgt attagctgca tggttggcat gtcggctttg tcgtacactt
       61 tgttaaacag tctggaggaa ataatgaaag aaaccgatat tgcaggcatt ttaacgtcca
      121 ctcacaccat tgcgctggtg ggggcaagcg acaaacccga tcgtcccagc tatcgcgtga
      181 tgaaatatct actcgaccag gggtatcacg tcattccggt ttcgccaaaa gttgccggca
      241 aaacgctgct gggccagcag gggtatggca cgctggcaga cgttccggaa aaggtcgata
      301 tggtcgatgt tttccgtaat tcagaagcgg cgtggggcgt ggcacaggaa gctattgcca
      361 ttggcgcgaa aaccctctgg atgcaactgg gggtgattaa tgagcaagcg gcagtactgg
      421 cacgggatgc cgggttaaat gtggtgatgg atcgctgccc ggctattgag atccctcgcc
      481 tgggcctggc caaataaaaa atccccggaa ggcaaaaacc ttccggggat tcgttcaggg
      541 attagttacg cagacgcggg gcctggagtt gtttgcggat ggtctgcgcc agctcatcca
      601 tggatggctg ttcaggatgt tcatcctgca attcgctact tagctgggct tcagccaggt
      661 aggtatgaac cggtaggccg ttatcgtcct ccatcaccac gtgataccac ggagcggcgc
      721 gaagctcgtc attcaccgcc agctcatcag gcgacggttc agagagcgaa taaaccgggt
      781 cgatatccac gaccactccg agataaccta acagggaatg gcggacctgc tggccgatac
      841 cgaatttgct ggcaatcata gtcacctccc gggaaatctt tacctttgat ataagggtaa
      901 aattccacat ttcaagttac atgacgcgac aggcaaaccc tttcagatat agcccttccg
      961 ggtaggtagc gatcaccgga tgatcggctg cctgacggaa ctgctctata aattgtacat
     1021 cacggccggc atcaattgcg gcatccgcga tgattttctg aaataaatcg ctggtcatca
     1081 gaccggaaca ggagaaagtc aggagaatac cgccttcatt cagcaactga atcgccagca
     1141 tgttgatatc tttatagcca cgacacgcgc ccatcaactg gcttttattc tcaacaaact
     1201 tcggcgggtc catcacgata acgtcaaatt tttcaccgcg atcgcgataa gtacgcagca
     1261 atttaaagac atcatcacgg acaaactcag ccttgctcag atccagtttg ttcagctcaa
     1321 cgttctgccg tgcaatatcc agcgcttcct gggaggtatc aacgctgaca acctggctgc
     1381 aaccgcccat cagtgccgat acggcgaaac caccggtata ggagaaacag ttcagcacac
     1441 gtttattttc aacgtagcgg cgggtagcca ggcggctatc acgctggtcc aggtagtagc
     1501 ccgttttgtg tccgtgctga atatccacca gcagtttcat gccgtgttct tcaatcggca
     1561 gcagggcagg tggcaactcg ccggtgacga ggccctgggt cagctccatc ccttcttttt
     1621 tacgtaccgc aacatcgctg cgatcgtaaa tcgcacattc cgggtacagc gtttgcaggg
     1681 cactaattaa tgccgcgcgc tgatattctg cgccagcact cagcagttgc agcaccagaa
     1741 aattaccgaa acgatcgata gtaatacccg gcaggccatc agattctccg gcgattaaac
     1801 gatagctgtc gaggccatct ttttgcgcca gccagtcacg ccatttttgt gcttgttgca
     1861 aacggcggga aaaaaaagca atgtcgatag actcagacgg gtcaaacgtc cagacgcgcg
     1921 cccggatttg cgaagctggt gaataagcgc cgcgtgctaa ccattttccc tgatgatcaa
     1981 caatatcgat ggtttcaccg aggctggctt taccttccat gcgggcaacg gccccggaaa
     2041 agacccacgg atggcgacga agtaatgatt tttcgcgccc tttggctaac actaaacgta
     2101 cactcataat ttacttttct gtcgatgcaa agaaatgggc gtcattgtcc ggagttttcc
     2161 atggatttgc aacgcgtcca ttaaggataa ggagagcagc aatgtcgaaa gtctgcataa
     2221 ttgcctgggt ttacgggcga gttcagggcg taggatttcg ctacaccaca cagtatgaag
     2281 cgaaaagact gggtttaacc gggtacgcca aaaatcttga cgatggcagc gtggaagtgg
     2341 ttgcctgcgg tgaagaaggg caggtggaaa aattaatgca gtggttaaag agtggcggcc
     2401 cgcgttctgc gcgtgtggaa cgggtgctta gcgagccgca tcatccctcg ggggaattag
     2461 ccgattttcg tatccgttaa atacatttta ccggttttgg caggccagca attttggtgg
     2521 cttgcttagc cggacctttc ggaaacagtc gatataaata gcggctgtta cctttttctt
     2581 cgccaaattt attcgccatc gcttttacca gcatacgaat cgccggagaa gtattgaatt
     2641 ccagatagaa atcacgcaca aaacgcacca cttcccagtg ttctggcgac agcgaaatcc
     2701 cttcgttctc tgcaatcacc acagccagcg gctcactcca ctggctgctt tctttgagat
     2761 agccttcggt atccgtttct atctctttac cttcgaagat cagcataatt attactacct
     2821 taatcagact ggcggcagtg taacaaaaaa caaagccccg cataaagcga ggctatgaaa
     2881 gtgttagcgg gtgagattaa tcgcggctag cgaagcccag aatgctcagc aggctgacga
     2941 agatgttgta cagcgaaaca tacaggctaa ccgtggcacg aatatagttc gtctcaccgc
     3001 catgaatgat gttgctggtt tcaaacaaga tagcgccaga ggagatcaga atgaagaccg
     3061 cgctgatcgc cagatgcaga gcaggcagtt gcaggaagat attagcaacc ataccaatca
     3121 gcaccaccac aatacccgcc atcagcatac cgccgaggaa cgacatatct ttgcgggtgg
     3181 tcagcacata tgcagagcag cagaagaaca ctaacgccgt tccgcccagt gccatagcga
     3241 ttacgtcacc cattccggca gacagatagg tgttcagaat aggtccgagg atataaccca
     3301 gaaaaccggt aaaggcgaat gcggagataa tcccggtcgg cttattcgcc gttttatagg
     3361 tcaggaacat caaaccatac atacccacca gcgtcagaat cagacccgga gatggcagca
     3421 tcagcaccgt actggcagtt gcggtaatcg ccgaaaaggc cagcgtcagg ctcagcagaa
     3481 aataggtatt acgcagcacc ttatgggtgc taagcagtga tgtacggtca tgtgaagaac
     3541 taacaatacg atccattgag tcactctcta tgacagatgt aattaattaa gcagcataat
     3601 gataatgcgt aagggcaccc agaagtttta cccatcttta cgcatttgat ctggaacagg
     3661 tttaacagcg gattatcagg tcattaagca aatataacgc cctgagaatt tcgacaggca
     3721 aaagaaaaag gggttagcat ttagctaacc ccttatctta tttggcggaa gcgcagagat
     3781 tcgaactctg gaaccctttc gggtcgccgg ttttcaagac cggtgccttc aaccgctcgg
     3841 ccacacttcc ggaatgacgc gcactataaa catcccgatg cggcgtgtaa acccctaatt
     3901 tgtttgtttg cctgaaaaac agccaaaagt gcattgatag cgtgaaataa cagcagattg
     3961 atcatttcat caccatgaat tccttctctt ttactcgttt agcaaccggc taaacatccc
     4021 caccgcccgg ccaaaagaaa aataggtcca tttttatcgc taaaagataa atccacacag
     4081 tttgtattgt tttgtgcaaa agtttcacta cgctttatta acaatacttt ctggcgacgt
     4141 gtgccagtgc agaaggatga gctttcgttt tcagcatctc acgtgaagcg atggtttgcc
     4201 ttgctacagg gacgtcgctt gccgaccata agcgcccggt gtcctgccgg tgtcgcaagg
     4261 agaagagacg tgcgatatga ataacgagga aacattttac caggccatgc ggcgtcaggg
     4321 cgttacccgg cgcagctttc tcaaatattg tagtctggct gccacgtcgc tgggattagg
     4381 cgcgggaatg gcaccaaaga ttgcctgggc gctggagaac aaaccccgca ttccggtggt
     4441 atggatccac ggtctggaat gcacctgctg taccgaatct tttatccgct ccgctcaccc
     4501 actggcgaag gacgtcatcc tttccctgat ttccctcgat tatgacgata ctttgatggc
     4561 tgccgccgga acccaggcgg aagaagtctt cgaagacatc atcacgcaat acaatggcaa
     4621 atatatcctc gcagtagaag gtaatccgcc gctgggcgag caggggatgt tctgtatcag
     4681 cagcggtcga ccgtttattg agaaactcaa acgtgccgct gccggagcca gcgcgattat
     4741 cgcctgggga acctgcgcgt cctggggctg cgtgcaggcc gcgcggccca atccgacgca
     4801 ggcaacgcct atcgacaaag tcatcaccga caaacccatt atcaaagtac ctggctgccc
     4861 gccgatcccg gatgtgatga gcgccatcat tacttacatg gtgacctttg atcgcttgcc
     4921 agatgtcgac agaatgggcc gtccgctgat gttctatggt cagcgaatcc acgataaatg
     4981 ctatcgccgc gcccacttcg acgccggaga gttcgtccag agttgggatg atgacgctgc
     5041 ccgcaaaggt tactgcctgt acaaaatggg ctgcaaaggg cctaccacct ataacgcctg
     5101 ttcctccaca cgctggaatg atggcgtttc tttcccaatc cagtctggtc acggctgcct
     5161 gggctgtgcg gaaaatggtt tctgggatcg cggttcgttc tacagccgcg tggtcgatat
     5221 tccgcaaatg ggtactcatt ccaccgccga taccgtcggt ttaaccgcgc ttggcgtggt
     5281 ggcagcggct gttggtgtgc acgcagtcgc cagcgccgtt gaccagcgca gacgtcataa
     5341 ccagcaacct acagaaaccg aacatcagcc aggcaatgag gataaacagg catgagcact
     5401 cagtacgaaa ctcagggata caccatcaat aatgccggac gccgcctggt ggtcgacccg
     5461 attacgcgca tcgaaggcca catgcgctgc gaagtgaata ttaacgatca gaatgtgatc
     5521 accaatgccg tctcctgcgg caccatgttt cgcgggctgg agatcattct gcaagggcgc
     5581 gacccgcgcg atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac tggcgtacac
     5641 gccctggctt cggtttacgc catcgaagat gctatcggta ttaaagtgcc ggacaacgcc
     5701 aatatcatcc gcaacattat gctggcaacg ctctggtgcc acgatcatct ggtgcacttc
     5761 tatcagcttg ccgggatgga ctggatcgat gtgttagatg cgctgaaagc cgacccgcgg
     5821 aaaacctccg aactggcgca aagtctctcc tcttggccga aatcatcccc tggctatttc
     5881 ttcgacgtac aaaaccgcct gaaaaaattt gttgaaggcg ggcagttggg gatcttccgc
     5941 aatggctact gggggcaccc gcagtacaaa ctgccgccag aagctaacct gatgggcttt
     6001 gcccactatc tcgaagctct cgatttccag cgtgaaattg tgaaaatcca cgcggtcttt
     6061 ggcggtaaaa acccgcatcc aaactggatt gtcggcggga tgccttgcgc cattaacatt
     6121 gacgaaagcg gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt gcagtcgatt
     6181 atcacccgca cggcagactt cattaacaac gtgatgatcc ccgacgcctt agccatcggt
     6241 cagttcaaca aaccgtggag cgaaatcggc acgggtcttt ctgataaatg tgtcctcagc
     6301 tacggcgcat tcccggatat tgccaacgac tttggtgaga aaagtctgct gatgcctggc
     6361 ggcgcggtga ttaacggcga cttcaacaat gtgctgccag tggatttggt tgatccgcag
     6421 caggtgcagg agtttgtcga tcacgcctgg tatcgttatc ccaacgatca ggtcgggcgt
     6481 catccattcg atggcattac cgacccgtgg tacaaccccg gcgatgtcaa aggcagcgat
     6541 accaacattc agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc gccacgctgg
     6601 cgcggtaacg cgatggaagt ggggccgctg gcacgcacgt taatcgctta tcacaaaggc
     6661 gatgctgcga ccgttgagtc ggtcgatcgc atgatgtcgg cgttgaacct gccgctttcc
     6721 ggtatccagt caacgttagg ccgcattttg tgccgcgcgc acgaagcgca gtgggccgca
     6781 ggtaagttgc agtatttctt cgacaagctg atgaccaacc tgaaaaacgg caatctcgcc
     6841 actgcttcca cggaaaaatg ggaacctgca acctggccga cagagtgccg tggtgtcggt
     6901 tttaccgaag cgccgcgcgg ggcgttaggc cactgggccg ccattcgcga tggcaagatt
     6961 gatctctacc agtgcgtggt gccgaccacc tggaacgcca gcccgcgcga tcccaaaggg
     7021 cagattggcg cttatgaagc ggcgctgatg aacaccaaaa tggcgatccc cgagcaaccg
     7081 ctggagatcc tgcgtactct gcacagcttt gacccgtgcc tcgcctgttc aacacacgtg
     7141 ctgggcgacg acggtagcga gctgatctcc gtgcaggtgc gttaacagcg aaggagaatc
     7201 atcatgcaac agaaaagcga caacgttgtc agccactatg tctttgaagc gccagtccgc
     7261 atctggcact ggttgacggt gttatgcatg gcggtgttga tggtcaccgg atactttatc
     7321 ggcaagccgc taccttccgt cagcggcgag gcgacgtatc tgttctatat gggctacatc
     7381 aggttaattc acttcagcgc cgggatggtt tttaccgtgg ttttgctgat gcggatctac
     7441 tgggcttttg ttggcaatcg atactcccgc gagctgttta tcgtgccggt atggcgtaaa
     7501 agctggtggc agggcgtgtg gtatgaaatc cgctggtatc tgtttctggc aaaacgtccg
     7561 agtgccgata taggccataa tcccatcgcc caggcggcga tgttcggcta tttcctgatg
     7621 tcggtcttta tgatcatcac tggttttgcg ctgtacagcg aacacagcca gtacgctatt
     7681 tttgcgccgt tccgttatgt ggtggaattt ttctactgga cgggtggcaa ctcaatggac
     7741 attcacagct ggcatcggct ggggatgtgg ctgattggcg cgtttgtgat cggtcatgtc
     7801 tacatggcac tgcgtgaaga catcatgtcc gacgacacgg tgatctccac catggtcaac
     7861 ggctaccgta gccacaaatt tggcaaaata agtaacaagg agcgttcatg agcgagcaac
     7921 gcgtggtggt catggggctg ggcaacctgc tgtgggccga tgaaggcttc ggcgtgcggg
     7981 tggcggaacg gctgtatgcc cattaccact ggcccgagta tgtggagatt gtcgatggcg
     8041 gtactcaggg actgaacttg ctggggtatg tcgaaagcgc cagccatctg ttgattctcg
     8101 atgccattga ctacgggctg gaacctggaa cgctgcgaac ctatgccgga gaacgcattc
     8161 cggcttatct cagcgcgaag aaaatgagcc tgcatcagaa cagtttctcc gaagtgttgg
     8221 cgctggcgga tatccgcgga catctgccag cacatattgc cctcgtcggt ctgcaacccg
     8281 caatgctcga cgactacggc ggtagcctga gcgaactggc acgggagcaa ctgcccgctg
     8341 cggaacaggc ggcgctggcg cagctggctg cgtggggaat tgtgccgcaa ccggctaatg
     8401 aatcgcgctg tctcaattat gactgtctgt cgatggaaaa ttacgaaggc gttcgcttgc
     8461 gccagtaccg gatgacacag gaggagcagg gatgagcaac gacacgccat ttgatgcgtt
     8521 gtggcaacga atgctggcgc gcggctggac gccagtcagt gaatcccgtc ttgacgactg
     8581 gcttacgcaa gcgccagacg gcgtggtgtt attaagcagt gacccgaaac gcacgccaga
     8641 ggtcagcgat aatccggtaa tgattggcga attactgcgc gagtttcccg actatacatg
     8701 gcaggtggcg attgctgacc ttgagcagag cgaagccatc ggcgatcgct ttggcgtctt
     8761 tcgctttcct gccactttag tgtttaccgg cggaaactat cgcggcgtgc tgaatggtat
     8821 tcacccgtgg gcggaactga taaacctgat gcgcgggctt gtcgaatcgc agcaggagcg
     8881 tgcctcatga gcgaaacttt tttccatctg ctggggccag gaacgcaacc gaacgatgac
     8941 agtttcagca tgaatccact gccgatcacc tgtcaggtga atgatgaacc gagtatggcg
     9001 gccctggagc aatgtgctca cagcccgcag gtgattgcgc tgttaaacga gttacaacat
     9061 caactaagcg aacgccaacc gccgttgggc gaggtgctgg cagtcgatct gttaaatctc
     9121 aacgccgacg atcgtcactt tatcaatacg cttctcgggg aaggggaagt gtcagtgcgc
     9181 attcagcagg ctgacgacag tgaaagtgaa atacaggagg cgatcttctg cggattatgg
     9241 cgggtgcgca gacgtcgcgg cgaaaagttg ctggaggaca aactggaggc tggctgcgcg
     9301 ccgctggcgt tgtggcaggc ggcaacgcaa aatctcttgc cgacagattc gctgttaccg
     9361 ccgcccattg atggcctgat gaatggccta ccgttggcgc atgagttact ggcacatgta
     9421 cgtaaccccg acgcgcagcc gcacagcatt aatctgacgc aattacccat cagcgaggct
     9481 gatcggcttt ttctctcacg tctctgtggg ccgggaaata ttcagattcg taccattggc
     9541 tatggcgaga gctatatcaa cgccacgggg ttacgccatg tctggcattt acgctgtacg
     9601 gacaccttaa aaggcccgtt actggaaagt tatgaaatct gcccaatacc ggaagtggtg
     9661 ctggcagcgc cagaagattt ggtcgactct gcgcagcggc ttagcgaggt atgtcagtgg
     9721 ctggcggaag ctgcaccgac gtaaaaagac ggtaagtatc gctttcagtc ttatgaatat
     9781 cgcaatcggc gaatacctct ggtcgtagag tttcaggata aagagggaga tctaccatta
     9841 tcgggttatt tttctctctt cgcctacagg agtgcgcatg tgggatgtca ttgatttatc
     9901 gcgctggcag tttgctctga ccgcgctgta tcacttttta tttgtacccc ttaccctggg
     9961 gctgattttt ttgctggcta tcatggaaac catttacgtg gtcaccggca aaacaatcta
    10021 ccgcgatatg acgcgcttct ggggtaagct cttcggtatc aattttgctc ttggcgtggc
    10081 taccggcctg accatggagt ttcagtttgg tactaactgg tcattctatt ccaactatgt
    10141 gggcgatatt tttggcgcac cactggcgat ggaagcatta atggccttct tcctcgaatc
    10201 cacctttgtc gggctgttct tcttcggctg gcagcggctg aataaatacc agcacctgct
    10261 ggtgacgtgg ctggtggcgt tcggttcaaa tctctctgca ttgtggatat tgaatgccaa
    10321 cggttggatg caatacccga ccggtgcgca ttttgatatc gacaccctgc gtatggagat
    10381 gaccagcttc agcgagctgg tctttaaccc ggtcagccag gtgaaatttg tgcacaccgt
    10441 aatggcgggc tacgtgaccg gggccatgtt tattatggcg atcagcgcct ggtatttact
    10501 gcgcggacgg gagcgcgatg tcgcattacg ttcgtttgcc atcggttccg tcttcggtac
    10561 tctggcgatt atcggtaccc tgcaactcgg agacagttct gcgtatgaag tcgcgcaagt
    10621 acaaccggta aaactggcgg cgatggaagg ggagtggcaa acggaacctg cacctgcacc
    10681 gttccatgtg gttgcctggc cggaacagga tcaagagcgt aacgcctttg ccatcaaaat
    10741 tcccgcgctg ctagggatcc tcgccactca ctcattagat aaacccgtgc cgggtctgaa
    10801 gaatttgatg gctgaaacct acccacgctt gcaacgcgga cgtatggcct ggctgttaat
    10861 gcaggaaata tcgcaaggca atcgtgagcc gcatgtgttg caggcattcc gggaactgga
    10921 aggtgacctg ggctacggca tgttgctctc ccgctatgcg ccggatatga atcatgtcac
    10981 agccgcacag taccaggcgg cgatgcgtgg cgcgatacct caggttgcgc cggtattctg
    11041 gagtttccgc atcatggtgg gctgtggttc cctgctgcta ctggtgatgc tgattgcgct
    11101 tgtccagacg ctgcgtggca aaatcgacca gcatcgctgg gtgctgaaaa tggcgctctg
    11161 gagtttgccg ttgccgtgga ttgcgattga agccgggtgg tttatgaccg agtttggtcg
    11221 tcagccgtgg gcgatacagg acatcttacc gacatactcc gcgcactccg ctttaaccac
    11281 aggacaactg gctttctcac tgatcatgat cgtagggctt tacaccctgt tcttaatcgc
    11341 cgaagtctac ctgatgcaga aatatgcccg tctggggccg agcgcgatgc agagtgaaca
    11401 accgacgcag caacaggggt aaaggagaaa atcatgtttg attatgaaac attgcgcttc
    11461 atctggtggc tgctgattgg cgtgatcctg gtggtcttta tgatctccga cggatttgac
    11521 atggggatcg gctgtctgct gccgctggtg gcgcgtaatg atgatgaacg ccggatagtg
    11581 ataaacagcg ttggtgcaca ctgggaaggc aaccaggtct ggttgatcct cgctggtggg
    11641 gcattatttg ccgcctggcc cagagtgtat gcagcggcgt tttccggctt ttatgtggcg
    11701 atgatcctgg tgctgtgctc actgttcttc cgcccgctgg cctttgatta tcgcggaaaa
    11761 atcgccgatg cacgctggcg taaaatgtgg gacgccggtc tggtcatcgg cagtctggtg
    11821 ccgccggtag tcttcggtat cgccttcggc aacttgttgc tcggcgtgcc gtttgccttc
    11881 acaccgcaat tacgcgtgga gtatcttggc agcttctggc aactgctgac gccattccct
    11941 ttattgtgcg gattgctcag ccttgggatg gtgattttgc aaggtggcgt ctggttacaa
    12001 ctgaaaactg ttggtgtgat tcatctgcgt tcacagctgg ccaccaaacg cgctgcactg
    12061 ttggtgatgc tgtgcttttt gctggcgggt tactggctgt gggtcggtat tgatggcttt
    12121 gtactgctcg cccaggatgc taacggtcct tccaatccgt taatgaaact ggtggcagtg
    12181 ctacctggtg cctggatgaa taatttcgtc gaatcgcccg ttttgtggat cttcccgctg
    12241 ctggggttct tctgcccatt gctgacggtg atggcgattt atcgtggtcg cccgggttgg
    12301 ggatttttaa tggcgtcatt gatgcaattt ggcgtgattt tcacggcagg catcacgctg
    12361 ttcccctttg tcatgccgtc aagcgtgagt ccaatctcca gcctgacgtt gtgggacagc
    12421 acttccagtc agctgacgct gagcattatg ttggtaatcg tgctgatatt tttgcccatt
    12481 gtgttgctct acactctctg gagctactac aaaatgtggg gacgcatgac aacagaaact
    12541 ctccgccgta acgaaaacga gttgtactaa ggagcaggaa caatgtggta tttactttgg
    12601 ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg gctggacccg
    12661 cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat tagcatcgca tcaggcaatc
    12721 aataatgtca gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa tcccattttt
    12781 atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc cggagctgaa
    12841 gctggaaagt gtggtgattg tcagtcgtca tggtgtgcgt gctccaacca aggccacgca
    12901 actgatgcag gatgtcaccc cagacgcatg gccaacctgg ccggtaaaac tgggttggct
    12961 gacaccgcgc ggtggtgagc taatcgccta tctcggacat taccaacgcc agcgtctggt
    13021 agccgacgga ttgctggcga aaaagggctg cccgcagtct ggtcaggtcg cgattattgc
    13081 tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc tggcacctga
    13141 ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt tatttaatcc
    13201 tctaaaaact ggcgtttgcc aactggataa cgcgaacgtg actgacgcga tcctcagcag
    13261 ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc gcgaactgga
    13321 acgggtgctt aattttccgc aatcaaactt gtgccttaaa cgtgagaaac aggacgaaag
    13381 ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca atgtctcatt
    13441 aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc aacaagcaca
    13501 gggaatgccg gagccggggt ggggaaggat caccgattca caccagtgga acaccttgct
    13561 aagtttgcat aacgcgcaat tttatttgct acaacgcacg ccagaggttg cccgcagccg
    13621 cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac cgcaaaaaca
    13681 ggcgtatggt gtgacattac ccacttcagt gctgtttatc gccggacacg atactaatct
    13741 ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt cccggtcagc cggataacac
    13801 gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata acagccagtg
    13861 gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa cgccgctgtc
    13921 attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag agcgaaatgc
    13981 gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac gcataccggc
    14041 gtgcagtttg taatgcataa aaaagagcat tcagttacct gaatgctctg aggctgatga
    14101 caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt tcacgccgca tccggccact
    14161 ttcagtttta ctctttctcg gagtaactat aaccgtaata gttatagccg taactgtaag
    14221 cggtgctggc gcgtttaatc acaccattga ggatagcgcc tttaatattg acgcctgcct
    14281 gttccagacg ctgcattgac aaactcacct ctttggcggt gttcaagcca aaacgcgcaa
    14341 ccagcaggct ggtgccaaca gaacgcccca cgaccgcggc atcactcacc gccagcatcg
    14401 gcggcgtatc gacaatcacc agatcgtaat ggtcgttcgc ccattccagt aattgacgca
    14461 tccgatcgcg catcagcagt tcagacgggt taggtggcac ctgaccgcga gtaatcacat
    14521 caaagcctcc tttgccaaaa tgctggatca ctttgttgag ctcatcttta cctgccagat
    14581 attccgacaa gccatgttca ttactcacgg taaacaggtt atgcgaataa ccacggcgta
    14641 agtcggcatc aataaataac actttttgat cggactgggc gatcaccgct gccagagttg
    14701 aactgacaaa cgttttacca ctgtctggcg tcgcaccggt gatcatcaga atgttattct
    14761 ccgtctccat catagcgaaa tgcagactgg ttcgtagcgc acgtacggct tccacagcag
    14821 aatccgccgg gttatccacc gccaggaagg ggatattttt agtacgatgg cgctgctgat
    14881 tagaaaataa atttttctta cgcagacggg tgcgtttatc cagccactcg gacattggga
    14941 tagtggcata aacgctgatg ccgtgctctt ccagttgttc cggggcttct acaccacgac
    15001 gcaacatcgc acgcgccagc acggcaccca cagaaataaa caggccaaga ataaaaccaa
    15061 gcaccacatt caacgctttt ttcggtttca ctggctgcgg ctgagtgact gccgggtcga
    15121 taatccgcac gttaccaatg gcactggatt tcgaaatact caactcctgc tggcggttaa
    15181 gtaattgcag atataccgca cggcccgctt ctacgtcacg acttaaacgc aacacttcct
    15241 gttgggtgga aggcattgcc gatacccgct tattcaggcg tttgcgttct tgctccagcg
    15301 tctggcgttt ttccagcagc gcacgataag ttgggtgatc tttcttatac agctgggaga
    15361 tctctgcctc gcggaaggtc agctcattga gttgattatc aacgttcaca atctgctcaa
    15421 gaacggcttt ggcttccagg ttaaggtcaa ccgaatcgcg ctgctggcga taaacgttga
    15481 gtttttcttc cgcctggtcc agctcgctgc gcacttcagg taactggcgc tgtaagaatt
    15541 caaggctttg tgaatcctgc gccgcctggc gagcgatatt ctgttgcaaa tagttgttag
    15601 cgatgctgtt cagaatacga gtaatcaact ggggatcatc accagtcatg gtaagttcca
    15661 gcatcccgct ttctttactg cgttcgctaa cggtaaaggt ttcctgcaat gcgttaatcg
    15721 cttccagttc ggtacgctgg ctcaggacaa actgtgttcc tggtttggcc ttaatgtccg
    15781 cgatagtcag cgcaacgcca tctttttcca gacgctggcc gaccatacca ttgacggtga
    15841 actcttcacc ttccagtgta tagtggccgt tttccccaac cgtgagtgtc agttgctgat
    15901 cctgaccatt cagttgtgga atatgcatcc agctgatcgc cagctcacct ggtttttctt
    15961 tggttaatct cgcccagccg cgacccacaa tcggaaaata cttctgctca actatgtcgc
    16021 gcagattcag ttcagcaatg gttttaccga gaatcatgcg cgattgcagc agttggatct
    16081 ccggtgcaga ctcgggcgat gagttaggga tcatatcgct caggccgctg agaatggcgt
    16141 tgccctgttt ttgctcaacc tggaccagag tatctgcctg ataaattggt gtacttaaca
    16201 gcgagtaagc gacagcgatc agcgtgaata acgcggtcac gctgataata aacttacggt
    16261 gatcccataa ctcgccgacc agacgaagca gatcgatctc attttcctga gtgctgcctg
    16321 gtggcgtatt catattttta gttgtcatcg tattttataa actcaaatta ccggctgagg
    16381 cgcttcgccc attcctgact ggcgcgctcc aacataccgt agacatgttc aaatgcgtcc
    16441 tgacttttac gatagggatc cgggatctct ttctgttcca gccattgccc aaacagcatt
    16501 gtcttcccgc ggacctcggg agcgattgcg gtaacctgag caatatgctc tgactccatt
    16561 gccagaatca gatcgtaatt tcgtgccatc tcagcagtga gcttacgtcc ggcatgtcct
    16621 tcaagagata cgccatgatt agcggcaaca tctgctgctg tcgcatctgc agggtgtttt
    16681 accagaccat gaacacctgc cgatttgact tttacgcctg gcagacgttt acgcagcaag
    16741 cgctcgccaa tgggcgaacg gcaaatgttg ccggtacaaa ccaccaggat tgagttaaat
    16801 tttagttggg ccatgtttga atccaccgtg aagtctctgt caggtcatgc accccggaaa
    16861 tcgtcggtac cagcagagaa atcacacggt tccaacgagc cagcggcgca gtagtgacat
    16921 aaacaatgtc gtaaggttca agctggaatt ccgttccgag tatcattgcc gatgcgtcct
    16981 tcgcattcag ctgataaata ttggcgattt tgccattctg cttgttttgc gttgcacgaa
    17041 taacaaaaat ccccgtcgca tcagcgacat cctgattcat accctcagcg ttacccagcg
    17101 cttccgccag ggtcataccg ctgcggtcca ttttcagcgt gctctgttta cccacttcac
    17161 ccatcacgaa gactttcaga tcatcgttgc gcggaataaa taaaatatcg cccgggtgca
    17221 gcagtttgtt ctggcgcaag tcgccacgct gcatcagcgc atagaggttc actttggtct
    17281 ttacgccatt ctgggtgagc accacgttgc gccagtctgc atctgcggtc agaccgcccg
    17341 cggcgttaat cgcatccatg atggtcagag gaatattggt aatcggctgc tgacccgatt
    17401 tgctgacttc gccggtgacg tacgcttttt gcgagcggaa cgccgcgacg ctgacatcga
    17461 cttgcgggct ttcaatgact gaatccagac gcgctgtaat ctcgttgcgt acctgggtaa
    17521 gtgttttccc ggccactttc aggcgaccaa tataggggta gaaaatcgca ccatcggcgt
    17581 taacccagtt gccggtatca ctggcgctac ggtattgccc tgcgggggtg gttagttccg
    17641 ggtgatccca gaccgtaacc atcaacacat ccccaatgcc gatacgatat tcatagtttg
    17701 cgatttgctg ctccagctcc gggttagcct gcgacataat cggtttggcg cgcaactgat
    17761 caatcaatcc tggcgtgacg ggatagatat tgaccatttt gtccaggtca tactgattgt
    17821 caggtagttc aatgacatcc ttgttactgg tcgataaatt ctgaccagga acaagggtac
    17881 atgctgtcaa agacagaaca gccaatgtca gtaccgaaaa cttaaaaatg ttctttttca
    17941 ttggtttata tttatcagtc aatgtaatgt tatgaattac gtcaggttat tgtccaaccg
    18001 gtacttctcg ctctgccgtc attggataga gctggtattt gcgtgacagc atttgtccac
    18061 catcacgcgt caatggtgtc cacgaaacca ccgcgcggtt gcggttaggt ccaatggtca
    18121 tcaagtcgaa tggaatcgag atataaaaac ctttactaaa gccgccttcg ccgtagtcat
    18181 ctttagatac gttactgatt gccgcccata cccctaccgc cacgccgctg tcaaagcgtt
    18241 tggcgacgtc gatcgttgcg cctttatctt ttgccagata ttgcccaacg ctaagtttca
    18301 tcagtacgcc attgagcgtc ggcgggttcc agtaagccgt cacgaaacct gttggcgtgg
    18361 aataatcggt gaaacgcatc atgttgtccc agtcacgctg cttcacgtag ttaacgtcca
    18421 cacccagcgc ccagctggca tctagcgggc gataaagcag ctcggaaccg acaccggcat
    18481 acatcgtttc cagataaccg ccatacacct ggccatagaa accattgcct aagtcagcaa
    18541 aatagttcgc ctgcaagttg ttgagataaa cgtcattgcg aacgtagtca cggatatgcg
    18601 tgcgcacgcg gggcagggta gagtccgcgg gcaacagcga agacttaaac ttgtcgtagt
    18661 tgttgtaaat attggtgaaa ataccgccat caagcagcag gtggtcggta aaccagtagc
    18721 gggcactgga catcagcccc agctggaaca tatagaaatc ttccgggccg cccagcgact
    18781 gtgaaagtgt tgggttgaaa gagtagctaa agcgatcttc acgaatacgg tagccccgac
    18841 caaaggcaga aagatcttct gcttcaacac gttgttgttg aagttgctct gattgaccag
    18901 gcgctgtacc agccagctgt ttgcgcaggc tggctacgtc ggtttcggta gtcaccatcg
    18961 ccatatgctc gcgcttttgc gtcacgctaa tcttctcaac gccttgcggc aggttattca
    19021 ccagaatccg gttggcacga tcgaccgctt cgcgagaatc acggtatttg tattgttgac
    19081 cagacatata cagcgtctta tcgcgcagct gaatttccgg tgcttcaaaa cctgcgttgt
    19141 acttcagcgc ggtaagttgg ttcgctactg tggtgtactg caatccttca gattcaggcg
    19201 caggttgata tgccggtttt ggcgtatcgc gcagggcagg gcgcagatcg ttgaaattgg
    19261 tccgtaacgt gaagccaaac atcaacgtgt taccgcgttc ataactcagg ttgagatctg
    19321 cccagctggc agcgcgataa actgcgccga cgttgaaatg gcttgcctga ggcagtttgc
    19381 cagcgaaatc attctggtaa ttgttgccat cgtattcgag tttcagacgc aggggattcc
    19441 acggcgtttg atactcaatg ccgccaaaga tggaagccgg gccacgaaag atatcgctaa
    19501 agctgatatc gcccgcatcg tgagactctg cgcgatgaca atatttatcg ctcacccggc
    19561 aaaacgggtt ggtaatattg cccgcattac cggcgtagcc ccatgccatc ccgagggtga
    19621 aatcaaatgg ccccgcttgc ttgctggcca ccagatactc accgtcaaac agacccgtac
    19681 cagcaatatc acgtttacca aacgccactt gcggtagcca gtaaccttct tcccacaggc
    19741 gaagtttaaa atcgaatgat ttgtctttgt atgactgatc gccgctgaaa tcttcccact
    19801 ggctatattt gcgtgtgcgc acatccgtat aacgaatggt gccttccagc catgggaaca
    19861 gcgccacgga ggtggagtag aaccggtatt gatcgttatc ccggtagttg acgctgaatt
    19921 caccttccgg tgcgatgcgc gcatttggca tctgcaacaa tcctgtgccg ccaaagtctg
    19981 attgcgacga acccagcgga tccgggtagg ttaatacttc agcatgacag gcactggaga
    20041 cggcaatggc caggcagctt aaaagataag aattcttctt catcagtcag gaacacgttg
    20101 cgttaaaaca gacacaatct ggtcatttaa atcagcatat ttttcgggca gtacatgcgc
    20161 agaaaagccc aaccaaagct ggctgccagg cggtggttcc acatgtcttt tattccacag
    20221 ggcaacaggg gcgacgacag tctcaccctc aggcgtaatc accataacgt tattcttgtc
    20281 cgcgcctgca aggcgggggt gatcttgcag ataatcggta acgctacggc ctgcctgcca
    20341 gggcaattgc ccagccccag aaaccgcacc cagcagagta atagtcaccg gacgctgtac
    20401 ggtatagagc gtatagtccc cgaccaacgg gggattgctg ttctcatcca ctcgaacaaa
    20461 atcagggtcc agtttgaccg gtaagcgacc agtgatattg agattgagta actgctggcg
    20521 cacggattta atcgttgccg ccacatcgtc gtcagcttct gcttcccagg aagcaagctg
    20581 tgccatgaca tgttggtagt ctttaagcgc tttagctttc gccgcagagt cggtcagcaa
    20641 ggcaccaggc caccagaggc gatcgcgcag ttgcggttgt gtcaccaact ggacaacatt
    20701 ttccacgggg ccaacggaaa gtgtctgttg ttcgccaggc agataaatag tcaccgttcc
    20761 ttgcgcaaag gcatggggtg tcattacgta aagtacgctg gcaatgaaat acgactgtaa
    20821 tttattcatg gtgcgggttt aagaaacgtc atttctacgg gaataacccc tgcgcctaac
    20881 atttgccgac tttgacgaac ctggccagta gccgaatcga cccagaatgt gttttgccac
    20941 tgtttttctg gccgcgtgga agtcacctct tcctgccaga cattacacag cacattgcga
    21001 cccgcaatat tcagcgtctc taatccagca aaggaaaaag tagaactcag ggtcgcagag
    21061 cgaaaatggt tgtcttcact ccagagaata tcgcgcgtcc agcggctgcc atcttttatt
    21121 gccagcgcgt tacgcagagg gtcctgcccg gaatttgtca cttccagcag attattatta
    21181 agtttgacgg tttttaaaag acgtccattg tgggtaacca gcatggcgtt atcctgggat
    21241 aaccatttgc tttgttcttg ttctatatat cccagtacca caaagattcg ttgcccttca
    21301 ttaaggcgta aatacatcgt ggaataaggc aacgcctgga tctgctgatc cgctacggtg
    21361 atatcctgat tatcgaaaag gctggcgcga aatgtatcga ccatactttg ctggctgtgc
    21421 gtacatcccg caagaaatag tgcgaaaatc gataaaataa gagggcgcac ctttaatcct
    21481 tgaaaggtta attaaaaaaa tgcaaatctc taaagcccgg tacgaatatc gcaaccgggt
    21541 atcatttaac tttaatttcg acgcgttaaa gcagtattag cgtgtagtgg acgtggtggt
    21601 tgaggtgtta tgagaaccgc catcgccact gtcatttgct gcaacaactg ccaacgtcgc
    21661 cacaccaaca gccgttgcca caccgacaac tgtcgccgtg ccgacagcgc cagcagccac
    21721 gccaccagtg gtagtagcag tcgtggttgc gccggtagtg gtcgttgtgg tgccagtagt
    21781 acccgtcgtt gcctcggtcc cgtttgttgc ttcaggggca gcaaacgcag ctggcgtcgt
    21841 caacatgaag gccataagga ttgcagaaag cttgtgtttc ataacttttc ctttattcat
    21901 cgcatggaca atacgtacgc ttgagatcag gattgtctac tttgagtata acatccttgt
    21961 gaaatctccc tttcctcata ccgcttgcac tatatactgc gggtaggaaa ggcgcgcaga
    22021 gggaaatata agattgttaa ctatccccct ctggaaatac gacttgtgag cataagataa
    22081 atcttagcaa aatacaaatt aaagataagg aaattgttac gaaagctatt aattattgtt
    22141 ggtaatatag tttcaagtgg tactataact ggctgttaaa tggcatttat aactattagg
    22201 tgcagagata ttcgcttaaa tggattagtt tctttgtgga aaagtaactg atgttataat
    22261 gtatgatgtt gactattgca ctattccaat aaaaccagtc agctttaaac aagcagcgtc
    22321 atattaagag agataaacat ttgccgctgt tggtcctcgc aggccattta cgcggcaaaa
    22381 ttccacacgt aatcctggta taagcacttc tgcgtcgcgg ggagtgaatg cggaaatatg
    22441 gacctgaact tctttacgac cgtcggaggg gataatgaat cctttgccgc ttttgcgatc
    22501 aaaggttttg acaattcctg tcattttacg ggacaaacaa attccttact gaaaatactg
    22561 cgctgcacta tacggggtta ataaaataaa gccagcgata tttaagaccg ccggacggct
    22621 aaaataaaat ttgcttaatc tcaattatca tgcgttaata gctgcgtcgg tttgaaagac
    22681 agacagcata caaagtagtt tactaaagca gttctcatta tcaggcatta tccccttctt
    22741 ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa agccctgttt gccgaaaggc
    22801 ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg gtttagtaaa atggtttaac
    22861 gcagataaag gttttggctt tatcactcct gatgatggca gcaaagacgt tttcgtccat
    22921 ttcaccgcca tccagagcaa tgaattccgc acgctgaacg aaaatcagaa agttgaattt
    22981 tctattgagc aggggcaacg tggccccgcg gcagcgaacg ttgttacgct ctaaggttgc
    23041 cgttattact caacatctcc atttccgctg tccatgttgt catggttcac agtaccgcac
    23101 atcggcattc gatgtgacgg agcgaaaccc tttgggcgct aagtgtattt tttgtaaatc
    23161 gacgatgatc acctttgata acgtcgcgct gcaaatacgc actgaccatg tgccgctgga
    23221 tttcacaaaa taatatcagg ctccctcgtg gagccttttt tatatctgcc ttatttttct
    23281 tcaacgctgt atgtatagta agcgataacc tgttgattat tgaatctttc ggggagatgg
    23341 cttataacat ttcttacctg accagggtac cgggaaccaa caccttactg gcgtgttgct
    23401 gtctttcaag accagaagag gttaacagtg aatattgaag agttaaaaaa acaagccgaa
    23461 acggaaatcg ccgactttat cgcgcaaaaa atcgccgagc tgaacaagaa tacagggaaa
    23521 gaagtctctg aaattcgctt caccgcacga gaaaaaatga ccgggcttga aagttatgat
    23581 gtcaaaatca aaataatgtg attttgtgaa catcacccgt gcgaggtgat gttccgcttg
    23641 ttgctaattt agtgaccaat cattggcgct tgtggaatta agcgtcggta caattcatcc
    23701 ggtaccgggc tttgccatac tcccgcatac attgcgtaac caatcaccgc aaacataatc
    23761 cccagaacca gtagcgtcat taaccagcca gacaacgcaa aggctttttt atttgccgca
    23821 ggtttttgca gtgaaaaggt caatgttgag gctaccggac atgactctac gcaagtcata
    23881 cagccggtac attccactgt tcgtacctga attaatttat cgaccgggat ccgtgatggg
    23941 caattttttg cgcatttgcc acagtcgata caactttcgg cattgcgacg aatcttaaac
    24001 ggcgacaata gcgaaaccac gcccatcagc gcgccatatg ggcaaagata acgacaccag
    24061 gcatggcgaa taaacaggct ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc
    24121 cccatatgac gaaagaaatc gagcatttta acgtccatca ccacgctgta gggcgacaac
    24181 ataaaatagt gaatcgcctg agcgggcatc aataacgcga tatagagaaa aaaactcaac
    24241 agcaaatact tcacgccgcg cagaggaata tccagccagc ggggaaggac acattgccga
    24301 ccaaacagtt tgttaccgag atcgccgatt aattcagaaa gcgtaccaac cgggcataac
    24361 catgagcaaa aggccttttt gagtaataga ctgatgacga taaaagcgac caataacagc
    24421 atcgcggcgg cgtggacgga cggtaactga cctgttacaa ggctatattt cagattcatc
    24481 agcccggcaa tcggtagcca gccttcgata cctcccggtc tggcgacaaa tgtcgtgcta
    24541 cttgccgttt cgtaatagcg cacccaatac cagaacgtga tggcaatata aatattcgtt
    24601 gccaacagta ataattgcgt cgctttacgc caggtcgtgg cattacgcca gtcattccac
    24661 ggtaatttgc cgcccgtcgt gcctggccgc cgctgccagc gggttctttt attctctgcc
    24721 atgattttgc cagtccgtta agttgtatac caaatgccac tattctagtt gttcttaact
    24781 ggctgatatt gattcaaatc gcgttcaggt ctttcttatg caaccatgct tccagagcgg
    24841 caacactgcg tgtaatttct tcgtgtggaa ggggggcaga taatggctgc tgctccagtt
    24901 gtgcgcatag ctggctggcg atacgcattc ccagactcga gcaactgctt tttagctgat
    24961 gcgcggcacg ctttattttc tcgctatcct gactggcgcg ggcaatgtcg atttcatcga
    25021 gaagcggcag ggcatgttgt gtaaataata ccagccattc gtggatcttc tccgtcccca
    25081 ttaactgagc atcttcattg agttgcgata catccagcga ttgatcgtta ttgacttgca
    25141 gttggagata gtgcgccagt aactgaccga gcacttcacg cggcaccggt ttagggataa
    25201 tcccgcggaa taatgaactg gtacgctggc gcagcgtttc gtcaatgaca tgggcgctaa
    25261 agccaatcaa aaccagcgac ggatattgct gtgccagttg tcgggcaagc gtaatgccgt
    25321 cgacatccgg cagatcaaaa tccaccagtg cggcagcaaa cggttcgcta ttttgcagtg
    25381 tctctaaagc ctgcgcggca ttgccaacag caacaacctg cgcaccactg gtgttcagca
    25441 tctcaacggt aattcgctgg gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt
    25501 caagacgcac cgcctgattg actgtttttg gcacgggtgc cgtggcaaca cgtaacggca
    25561 agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt tcgccgccca
    25621 tcgcctgggc cagacggcta ctgatagtca gccccagccc ggtgccgccg cgtttgccgc
    25681 ttacctgcac aaatggctgg aagatttctg ccagtttcgc gggatcaata ccgcagccgc
    25741 tgtcttccac ttcgaccagc cattgctcgc catcagtgcg actacgcagg ataatgtacc
    25801 cttcgtcagt aaaacgcagg gcgttgctca acaggttggt tataacctga cgaatacgtc
    25861 gtggatcgcc cattaacgcg cacggcatat catcggcaat tgccgttgcc aggcgaatcg
    25921 ggcgaccttt cacccgtccg ctcattaatt gcagggtact ttccagcagc gggcgcggtt
    25981 caaagggctc atcgctgacc gaaacattct tgccacctgc ttcgatagcg gaataatcga
    26041 gaatatcgtt gaggatggtc agcaacgatt caccagagtc agtaattgcc cgcaaatcat
    26101 cacgctgggc gttaagtgcg gggttatctg ccagcagttg agcagtgccg agaataccgt
    26161 acagcggtgt gcggatctca tggctcatcg ccgccagaaa cgccgatttt gcctggctgg
    26221 ctttttctgc ttccgcccgt gcctgtcggt gttctatcac cagttcctgc aattcagctg
    26281 tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg attcagtgca tgaacattgc
    26341 tgcgaaacgc atccatcagc cgcccgatgg tatccagctc ccgtacgcca gcggtttccg
    26401 ggaaagggga gtcaatatca ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa
    26461 gtggacgcgt gactgagcga taaaccacgc gccaaaggat cagaatcagt gcgcaaagtg
    26521 aaaccatccc cagcaataac aggctgtatt gcccgcgtgc actggctttt tccagatgtg
    26581 ccagtccatg ctgattgcgc agctcaatgg tgtcgaccag ctgactgact tcgctactaa
    26641 actgcgcgaa ctgggcgata ttattttggg cgagagtttg caggtgattg ctgatttcac
    26701 tgtcctgctg gaacagcgcc aacagatcgc tatattggct aacggtagtt aacgttgttg
    26761 cgacctgcgc acgaacaccc ggatcttcaa tgcgtatttg ccgacgttgc agaattttca
    26821 ccgcattatt gagctgcttt tccagcgttg gcgcattttt ctggatctgc tccagcccca
    26881 gattcatcac catttgctgc acccgcaggg cgctaaggcg cagttcattc atctggttaa
    26941 catactcaag atcgatatca atcagccgat cgagtgcact ttctgcggcc tgacgctgat
    27001 cttgttcgat caaatcgtaa atcccggcct gggtcgctcc ggcggaggtt gccgcattat
    27061 tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc agcgactatc tgctgactga
    27121 gttgctgttg ttgctggcgc agttgcaaac gctgccccac cagttcccct tgctgacgta
    27181 acgaacggga gatctcctgc tcctgttgtt caatagcggt ggtatcaaaa ccttgttccc
    27241 gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc ggtgagcatt cgcccctgcg
    27301 cctgccacat cttttcgtta tcggcactgg tcaggttctg cgcggcgaaa agttcccagg
    27361 cgctggcttc gctcaactgg cgcgccatat tcatggtagg aatcaatgcc tgagtattgt
    27421 ctttttccac ctggctgata aagcgcaggt tgtaccatcc caccagggta ctggtcaggg
    27481 ttaacagcgc catcagggca aagcccatcc agagtcttcg ggtcagggtt aaattcacgg
    27541 tcggtgcact ttaggtgaaa aaggttgagt cgcaaagcgg aatgcatcta gcataaagcc
    27601 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
    27661 tgccggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
    27721 aagcctctac gccgcttaaa gccaaacgcg catggaaact gtgcgcgctt tatcccagcc
    27781 tgaaagattc atattggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
    27841 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
    27901 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacct
    27961 catttcccga cctgcaaaag caggtagcaa gtctgccggt gatcgaactg gtaaatgcta
    28021 ttgatgctcc ccaggtgaaa agccgcgttg gtgtgccttg gtttcagatg ggctatcaac
    28081 cggggcgata tctggtgcaa tgggcgcacg gtaaaccact gaatgtgctg ttgatgcccg
    28141 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
    28201 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgta
    28261 acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc ggaacggcca
    28321 ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt accgtggtgt
    28381 cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg attatggctg
    28441 ccagcgatca aatggtctgg cagggggaac tggcagttga gcaggccatc aggcaattac
    28501 aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg ccgaaaaatg
    28561 ccgaccgaga acatatccgc cgctcgctgt caccaggggg atttcgtccg gtctattttt
    28621 atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc agtaaatccg
    28681 cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg tcagggtttt
    28741 ccacccgacg cgcagaaagc atacgtagca gacgttcacg gctgagaatt tcgcccggat
    28801 tcgtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcgccat
    28861 cccgctccag cgtatggcgc gacacattca ggcaataacc ggcaaagcga tagcagttgt
    28921 cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga tttttcaccc
    28981 gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca
    29041 gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc cccaccgttg
    29101 agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag ttgatatcca
    29161 gcagaattaa atctaccggc tgattctgca taatttcccg cagcccggca ccgctcgctg
    29221 taacggaaac ggtatacccc tcctgagtga agtaagattg taatcgcgcc tgggtaaccg
    29281 gctcatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat
    29341 atctgttcat attctgccgt aagccgttca tcctgaccag tgccgctgtt catatttgct
    29401 cattaagatc gcttcactaa accataattt tacaggggct attatgcgga aactctggaa
    29461 cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg ctggtcgcaa ttgggattgt
    29521 gattggcatt gcgctgattg tattgccaca cgttgggatc aaagtcacca gcacaaccga
    29581 attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa gaatataaac agtcggtgca
    29641 tttccagaac gcctccggcg tgcgagctga atgccatgac tgtcatatcc cgccggatat
    29701 tccaggcatg gtgaagcgca aactggaagc gagcaatgat atctaccaga cctttattgc
    29761 tcactccatt gatacacctg aaaaattcga agccaaacgc gcggaacttg ccgagcgtga
    29821 atgggcgcga atgaaagaaa acaactcggc aacctgccgc tcctgccata actacgatgc
    29881 gatggatcat gcgaagcagc atcctgaagc ggcacgtcag atgaaggtgg cagcgaaaga
    29941 taatcaatcc tgcatcgact gtcataaagg tattgcccac cagttaccgg atatgagtag
    30001 cggcttccgt aagcagttcg atgagctgcg cgccagtgct aatgacagtg gtgacacgct
    30061 gtactctatt gatattaagc caatttatgc ggcgaaaggc gataaagaag cctctggttc
    30121 tctgctgcct gcttcggaag tgaaagtcct taaacgtgac ggcgactggc tgcaaattga
    30181 aattaccggc tggacggaaa gcgccggacg tcagcgtgta ctcacccaat tcccaggtaa
    30241 acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag caggtaaaaa cgctggagaa
    30301 aaccaccgtt gccgacacca ataccgagtg gagcaagttg caggccactg cgtggatgaa
    30361 gaaaggcgac atggtgaacg atatcaaacc gatctgggct tatgcggatt cgttgtacaa
    30421 cggcacctgt aaccagtgcc acggcgcacc ggaaatcgcc cactttgacg ctaacggttg
    30481 gatcggcacg ctcaacggca tgattggctt taccagtctc gataaacgtg aagaacgcac
    30541 cttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca ggtaaggctc acggcgataa
    30601 gaaggaagaa aaataatgaa caataacgat ctctttcagg catcacgtcg gcgttttctg
    30661 gcacaactcg gcggcttaac cgtcgccggg atgctggggc cgtcattgtt aacgccgcgc
    30721 cgtgcgactg cggcgcaagc ggcgactgac gctgtcatct cgaaagaggg cattcttacc
    30781 gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg gtcgctttgt ggcggcaaaa
    30841 ccgttcgaac tggataaata tccgtcgaaa atgattgccg gattgccgga tcacgtacac
    30901 aacgcggcgc gtattcgtta tccgatggta cgcgtggact ggctgcgtaa gcgccatctc
    30961 agcgatacct cccagcgcgg tgataaccgt tttgtgcgcg tgagctggga tgaagccctc
    31021 gacatgttct atgaagaact ggaacgcgtg cagaaaactc acgggccgag tgccttgctg
    31081 accgccagtg gttggcaatc gacggggatg ttccataacg cttcggggat gctggcgaaa
    31141 gctattgcct tgcatggtaa tagcgttggt acgggcggag attactctac cggtgctgcg
    31201 caggtgatcc tgccgcgcgt ggttggctca atggaagtgt atgaacagca aacctcctgg
    31261 ccgctggtat tgcagaacag caaaaccatt gtgctgtggg gctctgattt gctgaaaaac
    31321 cagcaagcga actggtggtg cccggatcac gatgtttatg aatattacgc gcagctaaaa
    31381 gcgaaagtcg ccgccggtga aattgaggtc atcagcatcg atccggttgt cacatccacc
    31441 catgagtatc tggggcgcga gcatgtgaag cacattgcgg ttaacccgca aactgatgtg
    31501 ccgctgcaac tggcgctggc gtatacgctg tacagtgaaa acctgtacga caaaaacttc
    31561 ctcgctaact actgtgtggg ttttgagcag ttcctgccgt acctgctggg tgagaaagac
    31621 ggtcagccga aagatgccgc atgggctgaa aaactgaccg gcattgatgc cgaaaccatt
    31681 cgtgggctgg cgcggcagat ggcggcgaac agaacgcaaa ttattgctgg ctggtgcgtg
    31741 cagcgtatgc agcacggtga acagtgggcg tggatgattg tggttctggc ggcgatgctg
    31801 gggcaaattg gcctgccagg tggtggcttt ggttttggct ggcactataa cggcgcaggc
    31861 acgccggggc gtaaaggcgt tattctgagt ggtttctccg gctctacgtc gattccgcct
    31921 gttcacgaca acagtgatta caaaggctac agcagcacca ttccgattgc ccgttttatc
    31981 gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg gtaaatcggt aaaactgccg
    32041 ccgctgaaaa tgtgtatttt tgccggaact aacccattcc atcgccatca gcagatcaac
    32101 cgcattattg aaggctggcg caagctggaa acggttatcg ccatagataa ccagtggacc
    32161 tcaacctgcc gctttgccga tatcgtgctg cctgcgacca cgcagtttga gcgtaacgat
    32221 ctcgaccagt acggcaacca ctccaaccgt ggcattatcg ccatgaaaca ggtggtgccg
    32281 ccgcagttcg aggcgcgcaa cgactttgat attttccgcg agctgtgccg tcgctttaat
    32341 cgcgaagaag cctttaccga agggctggac gaaatgggct ggctgaaacg catctggcag
    32401 gaaggtgtac agcaaggcaa aggacgcggc gttcatctgc cagcgtttga tgacttctgg
    32461 aataacaaag agtacgtcga gtttgaccat ccgcagatgt ttgttcgcca ccaggcattc
    32521 cgcgaagatc cggatctcga accgctgggc acgccgagtg gcctgattga gatctactcg
    32581 aaaaccatcg ccgatatgaa ctacgacgat tgtcaggggc atccgatgtg gtttgagaaa
    32641 atcgaacgct cccacggtgg gcccggctcg cagaagtatc cgttgcatct gcaatctgtg
    32701 catccggatt ttcgacttca ctcgcagtta tgtgagtcgg aaactctgcg tcagcaatat
    32761 acggtagcgg gtaaagagcc agtattcatt aacccgcagg atgccagcgc gcgcggtatt
    32821 cgtaacggtg atgtggtacg cgtctttaac gctcgcggtc aggtgttggc aggggcagtg
    32881 gtttctgacc gctatgcacc cggtgtggca cgaattcacg aaggggcatg gtacgatcca
    32941 gataaaggcg gcgagcctgg tgcgctgtgc aaatacggta accccaacgt gttgaccatc
    33001 gacatcggta catcgcagct ggcgcaggcg accagtgcgc acactacgct ggtggaaatt
    33061 gagaagtaca acggaacagt ggagcaggtg acggcgttta acggccccgt ggagatggtg
    33121 gcgcagtgcg aatatgttcc cgcgtcgcag gtgaaatcat gaccacgctg acagcacaac
    33181 agattgcctg tatttacgcc tggctggcgc agttgttctc ccgtgaactg gacgatgaac
    33241 aactgacgca aatcgccagt gcgcagatgg ctgaatggtt ttcgttgctg aaaagcgaac
    33301 cgccgctggc tgcggcggtg aatgcgctgg aaaaccgtat tgccgctctg acagtacgtg
    33361 acgatgcccg tctggaactg gccgctgact tttgcggcct gtttctgatg accgacaaac
    33421 aagcggcgct gccgtatgca tcggcctaca aacaggacga gcaagagatt aaacgcttgt
    33481 tagttgaggc agggatggaa accagcggca atttcaacga accggcagat catctggcga
    33541 tctatctcga gttgttgagt catctgcatt tttcgctggg agaggggacc gttcctgcgc
    33601 gaagaatcga cagtttgcgg caaaaaacac tgacggcgct gtggcaatgg ttaccagagt
    33661 ttgttgcgcg ttgccatcag catgacagct ttggttttta cgcggcacta agccagttat
    33721 tgctggtgtt agtggagtgc gaccaccaaa acagataacg tcgtttgtgc gcctgaaaag
    33781 acgcgtttag cgtcgcatca ggcattatgg ggcagttgcc ggatgcgtca tcacatccgg
    33841 caaacacaca tcacggatga gttacaaacc gggaaagtcg ctggcgcagc aggcggtttt
    33901 cctgcttcag gtgcgcaata tcatccatta acgtcagcgc caccgcgatc ccaggccagt
    33961 ccagagccag ttcatgacgc aggcgtactg cgcgttgcac cacgatggcg gcatggtcgt
    34021 caaataccca ggttgtttcc tggatctcac gcggttcaac cacccccaaa ccgacaattt
    34081 cattcaactc ctcttcagag atgccggtat gcaggcaaaa ttcggtaata gtaaaagtca
    34141 ccgtaacatt agccattatg ctttccccca atctttacgt ggatcaaaag acgactgggc
    34201 gtctgccagt tgctgccaca gcgcggcagt gttttcatcc ggtttcggcg gcatcacgat
    34261 tttcagtacc gcatacagat cgccggtctg ttttttgctc accagacctt tgcctttaac
    34321 gcgcaatcgt tgcccggcct ggctgcctgg cgggatagtc agcaaaatgc tttctttcag
    34381 tgttggaacg gtgactttag cacccagcgc cgcttcccac gggctaaccg gcactacaat
    34441 ttccagatca tggccgacaa tatcgaacag cggatgtggc gcaatatgaa tcaccagcca
    34501 caaatcgcca tttggaccgc cgttttcgcc cggcgtcccc tggcctttca gacggatgcg
    34561 ttgaccattg ccgacgcccg ccgggatctt gacattcagc gttttcggaa tttcctgttc
    34621 gatcatgcca aaggcgttat aaaccggcag gttatagctg atggtacgct tatgctcagt
    34681 cagcgtttct tcgaggaata ccgccacttc gatttcaata tcgtggccgc gtgtggcggg
    34741 gcgttgacgg ctctggcggg catgctgacc gaaaattgac gagaagatat cgtcaaaatc
    34801 ttcggcgtta aaactctgac cgtcgctatg atggaactga cggttaaatt gcggatcgtt
    34861 gcgatgttgc cacatctgat catactcagc gcgacgttgt tcatcactta acacttccca
    34921 ggcttcagcg acctctttga agcgggcttc ggcatccggt tctttgctga catcaggatg
    34981 gtatttgcgg gccagtcgac gataggcggt cttgattgtc ttgagatcgt ccgtcggttt
    35041 cacgcccatg atggcgtaat aatcctttaa ttccatagcg ttatctcgcg taaatcaaca
    35101 caaattgaag gaacccctgt aaggtaactc ctataagtgt agggtaatcc tcaaaatttc
    35161 atatgccaac acagaatatg ttattgaaat catcgcggag aggaggtcgc ctcaaggtgg
    35221 ggtgctgaac atattttaaa caggtgaaaa agggtgagcg atttttgata gttgaaccag
    35281 gcactttaag tttaactggg gcgtcattat ttattaaatt ttatagacgc tatatatggg
    35341 tagtaatata catggaatta gttgcactgc aaataattat ttgaaacagg cctggaacga
    35401 tataaaaaat gagtacgaaa aaaatcaaac atattcaatc acgctttttg aaaacacact
    35461 ggtgtgtttt atgcggttat acaatgaact cagacgtaaa gtaaatgaag aggatactcc
    35521 atgtctggaa tgtgaatcac tagaaaaaga atttgaggaa atgcagaatg ataatgatct
    35581 atcattattt atgagaacat tgcgtactaa tgatacacaa atttattcag gggtttcagg
    35641 aggtattaca tatactatac aatatgttca aaatgttgat attgttagag tgtccttgcc
    35701 gggcagaggt tcagagtcta tcacagattt taaaggttat tattggtatg gctttatgga
    35761 atatattgaa aacattactg cgtgtgatga tgttttttct gagtattgtt tagatgatga
    35821 aaatatgagt atccagccag aatggataaa tacgccggga atatctgatt tagatactgg
    35881 tattgatttg tctggtatat cttttattca gagtgaaatt aataaaactt atggattaaa
    35941 atacgctcca gtagatggcg atggctattg tctgttaagg gccatactgg ttttaaaaga
    36001 acatgagtat tcatgggcgc tgggcagcca taagacgcaa aagcaagtat acgaagagtt
    36061 tattaaaatt gttgataaac aaacgatcga ggctcttgtt gatacggcat tcaatgatct
    36121 cagggaagat gtaaagacat tattcggcgt taatctacaa tctgacaaca aaattcaggg
    36181 gcagggtggt tttttatcat ggagtttttt gtcttttaaa aaagaattta ttgatagttg
    36241 cttgaatgac aaaaaatgca tactgcattt acccgaattt atatttaatg ataacaaggt
    36301 acgccttgtt ttagacaccg acccagagca gagggttaat gaggtgaaaa atttcctcac
    36361 tgctctttca gatagcattt gctcattatt tattgttaat agtaatatgg catcaatctc
    36421 cttggggaat gaatcctttt caacagatga agatcttgag tatggttatt taatgaacac
    36481 tggcaatcat tatgacgttt acctccctcc tgaacttttt gctcaggctt acaagttaaa
    36541 caataaggaa atgaatgcgc aactcgacta tttaaatcgt tatgcaattt actgacaagg
    36601 gcatgtgcta aaaaccattg ttattagcct cacttctctt attattggta ctattgcctc
    36661 tgtattagta acgccgctga tatcctgttt agccacaggt gtaatcatca gcggtgtacg
    36721 cgaggcaggg gataagcaga catagcttgc atcaaacctt acctgttttt aaagatgaat
    36781 ataaaaacag ccgctcaaca agctgttttg cgagcctgtt cccggaaaaa tgtcatattt
    36841 ctgtcacact ctttagtgat tgataacaaa agaggtgcca ggaatgaaca aaacgctaat
    36901 cgccgcagct gtggcaggga tagttttaat cgcttcaaac gctcaggcac aaaccgtacc
    36961 ggaaggctat cagctacagc aagtgctcat gatgagccgc cataacttac gtgcgccgct
    37021 ggcgaacaat ggcagtgtgc tggagcagtc gacgccgaat aaatggccag aatgggacgt
    37081 ccccggtggg caactcacca ccaaaggtgg cgtgctcgaa gtgtatatgg gccattacat
    37141 gcgtgaatgg ctggcagagc aggggatggt aaaatcgggg gaatgcccgc cgccggacac
    37201 cgtttatgcc tatgccaata gtctgcaacg tactgtcgcg accgcacagt tctttattac
    37261 cggcgcattt ccggggtgtg atattcctgt gcatcaccag gaaaaaatgg gaactatgga
    37321 cccaaccttt aacccggtga tcaccgatga ttccgccgca ttcagtgaac aggcggtggc
    37381 ggcaatggag aaagagctca gcaaactcca gcttaccgac agctaccaac tactggaaaa
    37441 aatcgttaac tataaagatt cccctgcctg taaagagaaa caacagtgtt cgctggtgga
    37501 tggcaaaaat acctttagcg ccaagtatca acaagaacct ggtgtttccg ggccgctgaa
    37561 agtcggcaac tcgctggtag atgcgtttac tttgcaatat tacgaaggtt ttccgatgga
    37621 tcaggtggcc tggggagaaa tcaaatctga ccagcagtgg aaggtgttgt cgaagcttaa
    37681 aaacggctac caggacagcc tgtttacctc accggaagtg gcgcgcaatg ttgcgaaacc
    37741 gctggtcagt tatatcgaca aagctctggt caccgatcgc accagcgcac cgaaaattac
    37801 agtgttggtt gggcacgact ccaacattgc ctctctgtta acggcgctgg atttcaaacc
    37861 gtatcagttg catgaccaga acgaacgcac gccgattggc ggcaaaatcg ttttccagcg
    37921 ttggcatgac agcaaagcca atcgcgattt gatgaaaatt gaatatgtgt accagagtgc
    37981 ggaacagtta cgtaatgccg atgcgttaac cctgcaggca cctgcgcagc gcgtgacgct
    38041 ggaattaagc ggttgcccga tagatgccaa cggtttctgc ccaatggata agtttgatag
    38101 cgtgttgaat gaggcggtga aataacagaa aactcccccg cgagaagtgg gggagtcgct
    38161 ggttaaacgt ttttacgttc gatggtctgt tcgccccaaa aaagcgaatc tttatcggtc
    38221 ttagcaaagg ctttgactaa cacttcatcg ctaccttctt cccaaatctt ttccgccatt
    38281 ttttcgtcgt acccggcgac ttcgaaaatg gcctcggcta tttccggcga cgtattgcgc
    38341 agagatgccc attcaccgac gtgatgagct ttcgcttctt gagttggcat gcgtatcctc
    38401 ctgttgaaga ttagccgtta agtttaactg ccagacctgc gacatattcc ccttgataac
    38461 gagcaataga cagttcttcc tggcttggct ggcgtgagcc gtcaccgcct gcgatggtgg
    38521 ttgcgccgta cggcgtaccg ccgcgaacct gtgaaacgtc aaataattcc tgcgctgcgt
    38581 agccaatggg gacaattacc atgccgtgat gcgcaagggt cgtccaggtt gaagtaatag
    38641 tttgttcctg accgccgcca gtaccggtgg aactaaagac gctcgccagt tttccgtata
    38701 gtgcgccgga agcccacagg ccgcccgtct ggtcgaggaa ggtacgcatt tgaccggaca
    38761 tgttgccaaa gcgggtaggt gtaccaaaaa taatggcgtc gtaatcggcc agttcttgcg
    38821 gggttgcaac cggtgcagtt tgcgttttac cgcctgcttt ttcaaataat tgcggcggca
    38881 tggtttccgg tacgcgctta acgacaactt ctgcgccatc gactttgctt gcaccctcag
    38941 cgactgcgcg tgccatcgtt tcaatatgtc cgtacatgga ataataaagc accagaactt
    39001 tagccatttc taaccactcc tcgtgttatc tctattccgt agcgattcgc taccacttat
    39061 ttaaagataa gacgtccttt tcagagtgca aatttcacaa ccacttattt gatttataac
    39121 aactttcaca agcacgtaat tttgtcgcaa aatgacacat ttttatctca tcgcgttttt
    39181 ttaatcataa gagcggctta tggataatta ttggagatga tatctattct cgctaataag
    39241 ctgttgcagg atattaccaa acgcgggtct gcccgcgtca gttcactaag cttagtccca
    39301 cgtagcgaaa atatggcagc cgccatacgc cgcgttaatt ctatgcaata tgatgtctat
    39361 acccagacgg aggtcagtaa tggcaaacca tcgaggcggt tccggcaatt ttgcagaaga
    39421 ccgcgaaaga gcatcagaag caggtaaaaa aggtggacag cacagcgggg ggaatttcaa
    39481 gaatgacccg cagcgcgcat ctgaagcagg taaaaaaggt ggcaagagca gtcacggtaa
    39541 aagtgacaac tagccggact aatcagtgac gaatgcattt ttgtctgaag ctcgtcaaaa
    39601 agtcatcacc gccggttacc ccggcggttt tctgtttatg gctcctgatg aacaacttct
    39661 ggcggtggaa cgtcaaccaa ccttctgctt aacaacgcat tgagtaaaat cgcgccaaag
    39721 gttgctgtac cgatccctcc caacgtaaaa ccgcccagcg tgagagcaaa atcacccgcg
    39781 cccagcacta aggttactgc gaccataatc aaattaccgt tctggcttaa atcgacacgg
    39841 ttttgtaccc agatccttgc gcctgcgacg gcaatcagcc cgaacacaac aattgaagca
    39901 ccaccaatca ccggtgcagg aatggtatgg atcagcgcgc caaatttcgg cgaaaagccc
    39961 aacagcatgg cgatgacggc agcagcaaca aacaccagcg tcgagtagac tttggtcacg
    40021 gccatcacac cgatattttc agcataggtg gtcacgccgc taccgccgac agagccggaa
    40081 agcatcgttg ccagaccatc gcctacgaat gcccgcccca tatacgggtc catattgcgt
    40141 ccggtcatcc cggcgactgc cttgagatga cctaagtttt ccgccaccag aatcaccgcc
    40201 acgggcgcaa tcagcatcat tgcctgacca ttaaaagcag gagtggaaaa atgtggcaaa
    40261 ccgaaccagg cagcatggct gacgagagta aaatcgacgg cttttcccag ccctaaaacg
    40321 ttggtcatca cgccatacag cagacaggcg acaattaatc ctacgagaat caataaccgc
    40381 tggatcatgc cacgggtaaa caccgccacc agcccaatac acagcaccgt cattaccgcc
    40441 atccagctat caaaggccga agccgataca cttttcactg cgataggcgc taagttcagg
    40501 ccaatcgcca tcaccaccgc acccgtcacc accggcggca ttagtcgttc aatccagcgt
    40561 gtaccaattt tcatcaccac caggccaatg acggtataaa ccagcccaca ggcgataatc
    40621 ccgcccagcg caatgctgat attcgggtta atgccctgac cgttaaagcc cgtcgcggca
    40681 atcaccacgc cgacaaaagc cgcgctggaa ccgagataac tggggacgcg cccgccggta
    40741 ataaagaaaa acagtaacgt gccgatcccc gacattaaaa tggaaagatt gggatccagc
    40801 cccatcagaa tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg ttgaacgccc
    40861 attactgccg tctgagcaaa cggcaatcgt tcatacggcg cgaccacgcc gctctctgta
    40921 gaggtcgatt ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg tctcctttag
    40981 gaggttaaca agcagggcgc atcagcgcgt gataactgcg atcgaaccac accagcccgt
    41041 agggtgtggc gtgacgatga atcgcttcga tggcgcaaaa cagaatgtcg tgggtgccga
    41101 cgctcaccac ctggctgata cggcaatcaa acgaaaccag agcctcttcc agttgcgggc
    41161 atccggtcac acccgtctgc cagcgggcgg cggcaaagcg gtgttccatg ggcgttttgc
    41221 cgccaaaaag atttgaaagc ggctcctgcc cggcgctcag tgtatttaca cacagcgttc
    41281 gattttcatt gaataccggc cagacggacg ccccacgatt caggcacacc agtaatgtgg
    41341 gcggcgtatc ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc ccggctggac
    41401 cgtccgtggt gatgatattg accgccgcgc ccatacagga catcgcatcg cgaaaggttt
    41461 gttgatcaac tatgttcata gtttgctcct tacaacagcc cgcaggcttc ttcaaaggac
    41521 agacgtggca ggcgcgcata aagtttgctg ctatcgccat agccgatatt aatcagcaga
    41581 ttgctcttca gcgtgctgcc tgcaaaaaaa gcgtcgtcaa cgtattgacg gtcaaaaccc
    41641 gacatcgggc cggtatccag tcccagcgca cggcaagcga cgatcagata ggccgcctgc
    41701 atggagctgt tgcgaaacgc tgtttcttcg gcaagttgtg ggctggaggt aaaccaactg
    41761 cgggcatcac cgtggggaaa cagtagtggt aaccgttcat aaaattcact gtcccaggca
    41821 acgatagcgg tgacgggcgc ggtcagggtt ttttgtagat tgccgctgga aagcgccggg
    41881 cgcagacgtt cttttccttc tacagtgcgg ataaacacga tccgtgccgg agaacagtta
    41941 gctgatgtcg gcccccattt catcagggca taaagctccc gtaacgtctc atcgctgacg
    42001 ggtgtctccc gccagccgtt gtgagtgcgg gcatcggtga acagggtgct aagcgcacct
    42061 gggctaacgg cttcgttcat agcaattcct tacagggcgg cttcgcggtg atgtaacagg
    42121 ctggcaagcc cgttgagtaa aagagcatta aacgtttcgg ggtcggtcac gttgcaggcg
    42181 tgtccgccat agcgcatcac cattttctgg ctatcgggca gggcggcatg aagctcactg
    42241 gaacatgctg atggcaccag cagatcatca ctggcgcaga tgatttgcac cgggcagcgg
    42301 atgcgatccg catggtgact aaagtcagcg cgtttgaggg cgttaagtcg acgcagtaaa
    42361 ttatttttgc cctgaaaatg cgccagtgcc agcgcgtctt ctgcctcaag gcgaggtgcg
    42421 cgggccgcca tccagtcggc gggatagagg aacaacggct gcgcttccac ccatgcctgc
    42481 gcgccgccgc tatacagtaa tcgttcgcga acctgaaaac agcggcgcgt atgggcgttt
    42541 attcgtagcc agccgttaac gctgatcagc acagttaccg acgcgggata atccagcgcc
    42601 agctgcattc ccaccagcgc accgagcgca tggccgacca ctgcgtaatg ctcaatccct
    42661 gcggctacca gcgcctgatg cagttccgct gccatctggg cgatactgta atcttctgcc
    42721 agcgtgtcgg gattattgcc ggtgccgcgc tggtcgtaac agactacctg atactcctgc
    42781 tccagcaccg ccagttgcgg taaccagtaa ctgccgctac ccccaagacc cgaaatcaac
    42841 accactacgg gcgcatcagc ataaggggga ggtgagagtg aaagtttcat cgcggcctca
    42901 cttggcgata tgcgcaattg tggcgatttc caccagcgcg tcaggtttta ccagtccgca
    42961 ctgaatgcag aatcgcgccg gtttatcacc cggaaaaaac tcggcgtaga tttcgttaat
    43021 cgcggcgtaa tttttccagt cggtaataaa gatgctgttg aaggtcacat ccgccatcgt
    43081 gccacccgcc gtctcgatca ccttgcggat agtttccaga acgtggcggg tttgcgcctt
    43141 tgggtcatcg gcaaacagca cgttattatg ttgatcaaaa gccagcgtac cggagacata
    43201 caccacgcca tcagccagcg tgccgggaac gaagggggcc agcggtgcgc tgctgccagc
    43261 gggaataatt acggattttg gcatcgttaa actccttaag cgatacgagc aaaggatgtg
    43321 ggagaaagcg cgtcgcagaa cgtttcgacg tcgctgaccc agccaaaaaa ggtttcgata
    43381 ttgaacaacg cggctttctg tgcaaattcc ggccccgcct ggtgtgttgc gtcttccagc
    43441 accacgccga aatactccag aaaaaagcca tcgcgtagcg tcgattcgac gcagacgttg
    43501 gtagcgatac cggtgaaaac cagatggcgt attccacggc tgcgcaaaat gctgtccagc
    43561 ggtgtattga agaaaccgct gtagcgcggc ttcggcagca caatatcgcc aggctgcggc
    43621 accagttcat ccaccagttg ataatcccag gagcctttcg ccagcaattt cccctgcagc
    43681 tgcggctgct tacgcatggt tttcagggcg ttcgatttat gaaaattcgg tgagccgggg
    43741 ccgccagctt cgacatactg ttcatcccag ccattttgaa accagatgat cagcatccct
    43801 gccgctcgcg ctgcggtcac ggcggtttga atgttggcaa tgaccgggcg agtggttgag
    43861 acatcaaacc cggcgagatc taagtagccg cctggcgtgg cataagcgtt ttgcatatcc
    43921 accacgatca gcgcactttg ctgcggatcg aaggtaatgg cttccggtcg agcggttaag
    43981 gttgtcatca tgccacctcc tgagtcagcg caggtagatg ggcgcggcac tgcatcagtg
    44041 gttgaatgcg ctcgccgaag gtttcgattc ccgacagaaa atcatcgaag gtcaacagca
    44101 cgccttcggc accaggcacg cttgcgactt catctaacat gcgcgcgaca ctggcgtaag
    44161 aaccgactaa cgtccccata ttgatgttta ccgccgaagt gggatcggcc atctgacgga
    44221 cgttggtatc agtaccggag cgggtatctt tctgactttg ttcggttagc cagcttaacg
    44281 cctcttcatc cgcgcccgct ttgtagtgtt cccatttggc gcgagcggca tcgtcggttt
    44341 catcggcaat caccataaac aacacataag aaccaacgtc gcgcccggtt tgctctgcgg
    44401 cctgtttcat ccgcgcagcg gtcggggcga aagccgtggg cgtatttacg cctttgccga
    44461 aacagaagtt gaaatcggca tactgggcgg agaacgccat gccagcgtcg ctttgcccgg
    44521 cgcagatcac tttcatgggg acactcggtt gcggactgac gcgacaatca ttcatggtga
    44581 aaaaatcgcc tttaaaatcg cttttccccg tgccccacag gtcgcgcagc acctgaacat
    44641 attcggtgag atagtcgtaa cgacgggaga aatagtcatc gccaggccag atacccatct
    44701 gctcatactc gggcttttgc cagccagtca cgaggttgac gccaaaacgc ccgccagaga
    44761 tggagtcgat ggttgcggcc atacgggcga cgattgccgg aggtaacgtt aaggtggcag
    44821 cagtggcgta aatctgaatg cgcgaggtca cggccgccag ccccgccatc aaggtgaacg
    44881 actcaaggtt gtgatcccag aactcagttt tgccgccaaa gccacgcagt ttgatcatcg
    44941 acagggcgaa atcgaaatgg tagtgctccg ctttttgcac aatggcttta ttcagttcaa
    45001 aggtcggcat gtactgcggc gcgtgggtcg aaatgagcca gccgttgttg ccaataggta
    45061 cgaatacgcc aattttcatc atcaacctct cttcgtctcg taaagtgaaa gtcagacggg
    45121 gcgctgcatc ctgcatatcc ttttcagccg cgtaatggct tgtttgcaaa gcggatgcca
    45181 gtttttaaaa agtgaatgtt attaatgtgt taacattgtg ttgtctaaaa tatctggtaa
    45241 aaagtggact aaacggtcaa aacagttgca cataaaacat gcatctgtgc gcgatgagag
    45301 tgcagaaggt cgaggccggg cgggggtttt gctatcctgt tgccaatcta caagagggga
    45361 gagcgcatga cgcaaggcgc agtgaaaaca acgggtaaac gttcgcgcgc agtaagcgcg
    45421 aagaaaaaag cgattcttag cgcagcactg gacacttttt cacaattcgg ttttcacggc
    45481 acaaggctgg agcagatcgc agagttggcg ggtgtttcaa aaaccaatct gctgtattac
    45541 ttcccgtcaa aagaggcgct gtatattgcc gtgctgcggc agattctcga tatctggctg
    45601 gcaccgttaa aagcgtttcg tgaagatttc gccccgctgg cggcgatcaa agagtacatc
    45661 cgtctgaagc tggaagtctc acgcgattat ccgcaggctt cgcgcctgtt ctgtatggag
    45721 atgctggcag gcgcgccgct gttaatggat gaactgacgg gcgatttgaa ggcattaatt
    45781 gatgagaaat cggcgctgat tgccggttgg gtcaaaagcg gcaaactcgc gccgattgat
    45841 ccgcagcatt tgattttcat gatttgggct tccactcaac attacgccga tttcgcccct
    45901 caggtggagg cggtgacagg cgcgacgttg cgcgatgaga tatttttcaa tcaaacggtt
    45961 gaaaacgtgc agcggattat tattgagggg attcgaccac gttaaagatg ccggaggagg
    46021 ttgtaacatc ctccggctac ctgtttaacc tatagtcatt aaactggcgt taccgccagc
    46081 ggcagcggta ttcacactca gcgaacgttc gatatacagc cgttccagaa ggatattgct
    46141 ttcgccacgg gcaaaaccct gcaccgaaac aattgcgcca tcccgcgcag caacggcttc
    46201 acacaatgcg cgaagctgat ccgaatcacc gtggaagatc accgcatcaa agggttgagc
    46261 ggttatattt tccgctttcg ccagttgaat acgttcgctg actgccgatg gcaatgcctt
    46321 tactagctga cgatgcagcg cgtcatccgg ccacagtacc tggctgccca ccgccagcac
    46381 ggcggcgagc tgagtcagcg catcctgctc atcatcggca atacacaaca cgcgctcacg
    46441 cggcagcagc gtccaggtgt tgcgttcacc cgtcggcccc ggcagcaatc gttgtgttcc
    46501 tgcctgcgcc agctcgccat attgcgtaca taacgcctgc aattctggac gatttgctgc
    46561 ccattcccgc agtgcattta gcggctgagt caatgcggct ttcaactgtg catcgaccgg
    46621 atactctgca tcctgacgcg cgagcgtcac tgccagcgca ctttccgggc gattcgccag
    46681 cagacggtag agatagagcg gaccgcctgc tttcggcccg gtaccggaca acccttcgcc
    46741 gccgaatggc tgcacaccca ccactgcgcc caccatatta cggttaacat acaggttacc
    46801 aacatgggcc gagccagtga cctgggcgat ggtttcatca atacgcgtat ggacaccaag
    46861 cgtcagacca tagccggaag cgttaatctg ctcgatcagc tctggtagct ggttacggtt
    46921 gtaacgcacc acatgcagca ccggaccaaa gacctctttt tgcaattcgg caaagtcatc
    46981 cagttcgatc agcgtcgggg cgacaaaggt gccgctttgc cattcacggg catcttcgct
    47041 gttttcccgc accgcctgga acaccggacg gcctttgcta cgcatggtct gaatatggcg
    47101 ctcaatattg gctttcgctt cgctatcaat caccggaccg atatcggtgg tcaggcgacc
    47161 cggattaccc atccggcatt cggccattgc gccgcgcagc attttcaacg tgtggtcggc
    47221 aatctcatct tgcaggcaca gcacgcgcag cgccgaacaa cgctgacccg cactgtcgaa
    47281 cgccgaggcc agtacatcca cgacgacctg ttcggtcagt gctgaagaat cgacaatcat
    47341 cgcgttcatg ccgccggttt cagcgatgag cggaataggg cgaccctgag cgtccaggcg
    47401 gctggcgata ttgcgctgca gtaacgtagc gacttcggtt gaaccggtaa acatcacccc
    47461 gcgtacgcga tcatcacccg tcagttgcgc gcccacggtt tccccctgac ctggcagcaa
    47521 ctgcaccacg cctggcggta cacccgcttc cagcaaaatg gcgatccctt gcgcggcaat
    47581 cagcggcgtt tgttctgccg gttttgccag cacgctgtta cctgccgcca gtgcggcagc
    47641 aatctgcccg gtgaaaatag ccagcgggaa gttccacgga ctgatacaca ccacaggccc
    47701 taatggacgg tgggtttcgt tagcgaaatc atcccgcacc tgtccggcat agtagtggag
    47761 aaaatcgacc gcttcgcgca cttcggcaat ggcgttactg aaggtttttc cggcttcacg
    47821 caccagaata ccaatcagtt gctgcatctg gctttccatt agcacggcag cgcgatgcaa
    47881 aatcgccgcg cgttcaaccg gaggagtggc aaaccagatt ggcgcgttat taaccgcact
    47941 ttccagcgcc tgttctacct cacgcggcgt ggcttcacgc acaaagccca caatatcttt
    48001 cggttccgca gggttaataa cgggcgacat ctcacctgcc gctaccggtt gttccagcat
    48061 tggcaaggcc tgccattttt gcagtgcact attgagcagg gcagaagaga gcgaggccag
    48121 ccggtgttcg ttagcgagat ccagccctgc cgagttgtcg cgcccgtgac cgtaaagatc
    48181 gcgcggcagg ggaattttcg gatgcggtaa tccagtttgc ccttcctgtt gcgccagttt
    48241 ttctacagca gtgaccggat cggcgaccag ttcatccagt ggcaaagagg tgtcggcaat
    48301 acggttaaca aacgaggtgt tagcaccgtt ttccagcagg cgacgcacca gatacgccaa
    48361 cagcgtttca tgggtaccga ccggagcata aatacgacac ggacggttaa gtttgccgtc
    48421 ggcaactttc ccggtgacct gctcatacag tggctcgccc ataccatgca ggcactggaa
    48481 ctcgtactga cccggatagt agttctgacc tgccagttga taaatcgctg ccagcgtatg
    48541 ggcgttgtgc gtcgcgaact gcgggtagat tagattcggc accgccagca gctttttcgc
    48601 acaggcgaga tatgaaacgt cggtatacac cttgcgggta taaaccggat agccttcaag
    48661 gccgtccatc tgcgcacgct tgatttcact atcccagtac gcgcctttca ccaggcgaat
    48721 catcagacgg cgacggctgc gggtggcgag atcaatcagg taatcaatca ccagtgggca
    48781 gcgtttttga taagcctgaa taacaaaacc gataccgttc cagcctgcca gctcaggctc
    48841 gaaacagagt ttttccagca gatcgaggga gatctccaga cgatcggcct cttcggcgtc
    48901 aatgttgata ccaatatcgt actgacgcgc cagcagggtg agtgatttca gacgtgggta
    48961 aagctcttcc attacccggt catactgggc gcggctgtaa cgcggatgca gcgccgacag
    49021 cttgattgaa atgcccggac cttcatagat gccacgacca ttggacgctt taccaatggc
    49081 gtgaatcgcc tgctgatagg aaaccatata cgcttgtgca tctgcggcag tcagtgcagc
    49141 ttcgccaagc atatcgtaag agtaacggaa acctttctct tccagcttgc gggcattggc
    49201 taacgcttcc gcgatggttt cgccagtgac gaactgctcg cccatcaggc gcatcgccat
    49261 atccacacct ttgcggatca gcggttcacc gcttttaccg ataatgcggt tcagcgagcg
    49321 ggagaggctg gcttcgttat gggtggaaac cagtttgcca gtaaacagca gcccccaggt
    49381 ggcggcatta acaaacaaag acgggctacg accaatgtgt gattgccagt taccgttgct
    49441 gattttgtcg cgaattaacg catcacgggt ggctttgtcg ggaatacgca acaacgcttc
    49501 cgccagacac atcagcgcca cgccttcctg cgatgacaac gaaaactcct gcaataaccc
    49561 ctgaaccata cctgcgcgac cactggcgtt tttttgatta cgcagtttat cggccagttg
    49621 atacgccagt ttgtgcgcct gttcagcaac tggctgcggc aggcgggctt gttccagcag
    49681 catagaaacc gcttcggttt ccgggcggcg ataggccgct gtgatcgcgg cgcgggaaac
    49741 cgactggggc aaaatttgct cggcaaagtc gaggaatggc tggtgtggtt cctctgccgg
    49801 agtcggtgct tcatcgctct cattggcagc gccagaaagc agcgcaggta gctccggcag
    49861 agtatcgctg ttttccagtt gttcgagata agaaaaaatc gcctgcttaa ttaaccagtg
    49921 tggcgtgcga tcgatacgtg tcgcggcaga cttaatacgc tcacgcgtcg cgtcgtccag
    49981 cttaaccccc atggtggtgg ttcccatgcc attactcctg ttgttctgaa aggtgcaact
    50041 taacgttatc gtgaaatatc catgatgttg caactttgtg caaccatgtt aaatgtgaca
    50101 tgcgtagcaa gcttaaaaat gaatgaaatg ttaataaaag aaatcgatat gacagggatt
    50161 aaaaaaataa ctcagacttt ttctctgcgg cagttaacat ttttgaaagg tgcaaccgca
    50221 aaaaatgtga gagagtgcaa cctgatgaaa aatagtgtcg ctgagcacta aaatttaatg
    50281 taaatggtgt gttaaatcga ttgtgaataa ccagcgcttc cggcaggata cggtcgccct
    50341 ggtaaaacat aaactctgtt accccgttcc ggtggcagat ataacggcaa gtttcgacat
    50401 tgccgataat aattttttgg agactttaga tggctattag cacaccgatg ttggtgacat
    50461 tttgtgtcta tatctttggc atgatattga ttgggtttat cgcctggcga tcaacgaaaa
    50521 actttgacga ctatattctg ggcggtcgta gccttgggcc attcgtgacg gcattatcgg
    50581 cgggtgcgtc ggatatgagc ggctggctgt taatggggtt gccgggcgcg gtttttcttt
    50641 ccgggatttc cgaaagttgg atcgccattg gcctgacatt aggcgcgtgg atcaactgga
    50701 agctggtggc ggggcggttg cgtgtgcata ccgaatacaa caataacgcc ttaacattgc
    50761 cggattattt caccgggcgc tttgaagata aaagccgcat tttgcgcatt atctccgcgc
    50821 tggtgatttt gctgttcttc accatttatt gcgcttcggg cattgtggca ggcgcgcgtc
    50881 tgtttgaaag tacctttggc atgagctacg aaacggctct gtgggcggga gctgcggcga
    50941 cgatccttta cacctttatt ggcggtttcc tcgcggtgag ctggactgac actgtacagg
    51001 ccagcctgat gatttttgcc ctgatcctga cgccggttat cgtcattatc agtgtcggtg
    51061 gctttggtga ctcgctggaa gtgatcaaac aaaagagcat cgaaaacgtt gatatgctca
    51121 aagggctgaa ctttgtcgcc attatctcac tgatgggttg ggggctgggt tacttcgggc
    51181 agccgcacat cctggcgcgt tttatggcgg cagactctca ccacagcatt gtccatgcgc
    51241 gtcgtatcag tatgacctgg atgatcctct gcctggcagg ggcggtagct gtcggcttct
    51301 ttggtattgc ttactttaat gagcacccgg cggtagcagg tgcggtaaac cagaatgctg
    51361 agcgcgtgtt tatcgaactg gcgcaaattc tgtttaaccc gtggattgcc gggattctgc
    51421 tgtcggcaat tctggcggcg gtaatgtcaa ccttaagttg ccagctgctg gtgtgctcca
    51481 gtgcgattac cgaagattta tacaaagcgt ttctgcgtaa acatgccagc cagaaagagc
    51541 tggtgtgggt agggcgtgtg atggtgctgg tggtggcgct ggtggcgatt gcgctggcgg
    51601 caaacccgga aaaccgcgtg ctgggcttag tgagctacgc gtgggcaggc tttggcgcgg
    51661 cgtttggtcc ggtggtgctg ttctcggtga tgtggtcacg catgacgcgc aacggtgcgc
    51721 tggcggggat gatcatcggt gcgctgacgg ttatcgtctg gaagcagttc ggctggctgg
    51781 gactctacga aattattccg ggcttcatct tcggcagtat cgggattgta gtgtttagtt
    51841 tgctgggtaa agcgccgtca gcggcgatgc aaaaacgctt tgccgaggcc gatgcgcact
    51901 atcattcggc tccgccgtca cggttgcagg aaagctaagg gacttagcct gcggcggttt
    51961 tgtttggctt cagcagcggg ttgcgctccc ttaatgtgcc tcgccatata aattgaatgg
    52021 tgcagggagc gcgcaggggg cggccaatcg ccgcggcccc ctgctgtccc ggccttcggg
    52081 gaacgcttca gcgattttga cgccaccaac acccgagctg ttattatgtt ccgggcaaaa
    52141 agttagattt gataatcgag gatggacgaa attgcttgat acacccgctt atcagtttta
    52201 catggaagtt ttgatgtatt gagtcaggac ggttttgtcg gctggatacg gcgtttacgc
    52261 ggcatccggc aagaacacat ggctcgttgt aaacaatccc atctttgtac ctggaataat
    52321 cgtttatatc ccctggcatt acctctcttt gtttacattt caacatcatt ttataaacat
    52381 tccgcttgta tttttcttta ccgtaatgat aatcgctatc actgcgattt acttttcttt
    52441 gcatagattg actcagaaaa acgtttaagg gtgggtggca tgtttgttcc gtttctcatt
    52501 atgttgcgcg aaggacttga agccgcgctg attgtcagtt tgattgccag ctatcttaag
    52561 cgtacccagc gaggccgatg gattggtgtg atgtggattg gcgtgttgct tgccgctgcg
    52621 ttgtgcctgg gcttggggat cttcattaac gaaaccaccg gcgaatttcc gcaaaaagag
    52681 caggaactgt ttgaaggtat cgtggcggtg atcgccgtgg tgatccttac ctggatggtt
    52741 ttctggatgc gcaaagtgtc gcgcaacgtc aaagtacaac tggagcaggc agtcgatagc
    52801 gcattgcagc gtggaaatca tcatggctgg gcgctggtga tgatggtctt ttttgccgtt
    52861 gcaagggaag ggctggagtc ggtctttttc ctgctggcgg catttcaaca agatgtcggg
    52921 atctggccgc cgctgggtgc aatgctcggt cttgctactg ccgtggtgct aggcttcctg
    52981 ctctactggg gcggtattcg cctcaatctt ggtgcatttt ttaaatggac cagcctgttt
    53041 attctcttcg tcgccgcagg gctggcggct ggtgccattc gcgcatttca tgaagccgga
    53101 ttgtggaacc actttcagga aatcgccttc gatatgagtg cggtgctctc aactcactcg
    53161 ctgtttggca cgctgatgga agggattttt ggctatcagg aagcgccgag cgtcagcgaa
    53221 gtcgccgtct ggtttattta tctcatcccg gcgctggtgg catttgctct gccaccacgc
    53281 gcaggggcga cagcgtctcg ctccgcgtaa caaatacgac gcaaactctt gcttagttac
    53341 aacatacttt aaagagatag tctcgtcatg accattaact tccgccgtaa cgcattgcag
    53401 ttgagcgtgg ctgcgctgtt ctcttctgct tttatggcta acgccgctaa tgtgccgcag
    53461 gtcaaggtga ccgtgaccga taagcagtgc gaaccgatga ccattacggt taacgccggg
    53521 aaaacacagt tcattattca gaaccacagc cagaaggcgc tggagtggga gatcctcaaa
    53581 ggcgtgatgg tggtggaaga gcgggaaaat atcgcccctg gctttagcca gaaaatgacg
    53641 gcgaatttac agcctggcga atacgatatg acctgcggtc tgctgactaa cccgaaaggg
    53701 aagttgatcg tcaaaggtga ggcaacggcg gatgcggcgc aaagtgatgc gctgttaagt
    53761 cttggtggtg caattactgc atataaagcg tatgtcatgg cggaaaccac gcagctggtg
    53821 accgacacga aagcctttac tgacgctatt aaagcaggcg atatcgaaaa agcgaaagca
    53881 ctgtatgcgc cgacgcgcca gcactatgag cgcatcgaac cgattgctga actgttctcc
    53941 gatctggatg gcagcattga cgcccgtgaa gatgattacg agcaaaaagc cgccgatccg
    54001 aaattcaccg gtttccaccg tctggaaaaa gcattgtttg gcgacaacac caccaaaggc
    54061 atggatcagt acgctgacca gctttatacc gatgtggtcg atttgcaaaa acgcatcagt
    54121 gaactggctt tcccaccttc aaaagtggtc ggcggtgcag ccggactgat tgaggaagtg
    54181 gcagccagca aaatcagcgg tgaagaagat cgctacagcc acaccgatct gtgggatttc
    54241 caggctaacg ttgaaggctc gcagaaaatt gtcgatctgc tgcgtccaca actgcaaaaa
    54301 gccaacccgg aactgttggc aaaagtcgat gccaacttta aaaaggtcga taccattctg
    54361 gcgaaatacc gtactaaaga cggttttgaa acctacgaca aattgaccga tgccgaccgg
    54421 aatgcactga aaggaccgat tactgcgctg gcggaagatc tggcgcaact tcgcggtgtg
    54481 ctgggattgg attaagcatt atgcagtatg aagataaaaa cggcgtgaat gaaccgtcac
    54541 gccgacgttt actgaaaggc attggtgcgc tggcgctggc gggaagttgt ccggtcgctc
    54601 atgcacaaaa aacgcaaagt gcgccgggta cgctttcacc ggatgcacgc aatgagaaac
    54661 agccgtttta tggtgagcat caggcaggga tcctgacgcc acaacaggcc gcaatgatgc
    54721 tggtggcgtt tgatgtgctt gccagcgata aacccgatct tgaacggttg tttcgcttgt
    54781 tgactcagcg ttttgctttt ctgactcagg gcggagcggc accagaaacg ccaaatccgc
    54841 gcctgccacc actcgattcc ggcattcttg gcggctacat tgcgcccgat aatctcacca
    54901 tcacgttatc ggtgggtcac tcattgtttg atgagcgctt tggccttgcg ccacagatgc
    54961 cgaaaaagtt gcagaagatg acgcgttttc ccaacgactc gctggatgcg gcgttatgtc
    55021 atggtgatgt gttgctacaa atttgcgcca acacccagga cacggtgatc catgcgctgc
    55081 gcgatatcat caaacatacg ccagatttgc tcagcgtgcg ctggaagcgg gaagggttta
    55141 tttccgatca cgcggcgcgt agtaaaggca aagagacgcc gattaatttg ctgggtttca
    55201 aagacggcac tgccaatccc gatagccaga atgataagtt gatgcaaaaa gtggtgtggg
    55261 taacggcaga tcagcaggag ccagcgtgga caatcggtgg cagctatcag gcagtacgct
    55321 tgattcagtt tcgagtggaa ttttgggaca gaactccgct gaaagaacag cagacgattt
    55381 ttggtcgtga taagcaaacc ggtgcgccgc tgggaatgca gcatgagcat gatgttcccg
    55441 attacgccag cgatccggaa gggaaggtga ttgcgctgga cagccatatc cggctggcga
    55501 atccccgcac ggcggagagt gagtccagcc tgatgctgcg tcgtggctac agttattcac
    55561 tgggcgtcac caactccggg caactggata tggggctact gtttgtctgt taccaacacg
    55621 atctggaaaa aggcttcctg actgtacaaa aaaggctcaa tggcgaagcg ctggaggaat
    55681 acgttaaacc tatcggcggc ggttattttt ttgcgctgcc gggagtgaag gacgcgaacg
    55741 attatctcgg aagcgcgtta ttgcgggttt aatgtttata gacggataag gcgttcttgc
    55801 gcagatgcct gatgcgatgc ttgcgcgtct tcttatgcct acaatcggtg cgggttcggt
    55861 aggcggataa ggcgttcacg ccgcatccgg cgttcgtgcg cagatgcctg atgcgaatcc
    55921 tgctgaaagc acacagcttt tttcatatct gtcatcactc tgtcatcttt ccagtagaaa
    55981 ctaatgtcac tgaaatggtg ttttatggtt aaatataagt gaatatattg ttgcaatgaa
    56041 tgcgagatct gttgtaatta ttaagtagca gcggaagttc ccggcagtga tagtcagtca
    56101 ctatggagat cgcggatggt aacgtcctgt tctggacaat gtcttgttaa gctacgctcc
    56161 accacttgcg gagatttctc ctccggtagc aacttcgtca ctctgtattt ttctcacttc
    56221 ctctctttac tcgtgttacc gacgccgcaa acggtgtgcg tagccgtttt tcggcatttt
    56281 atccaaaagc aaactatggc ttacaaggaa gccaaccctc tgatgttcgt gcgcataatc
    56341 gcgctgccaa cggcgcgtgt gatgaataca aacaactcaa ggtgctctcc atgggaagac
    56401 aaaaagcagt gatcaaagct cgtcgtgagg caaaacgtgt gctgagacgg gattcgcgca
    56461 gccataagca gcgtgaagaa gaatcggtca cctcgcttgt gcagatgggc ggcgtagaag
    56521 ccattggtat ggcccgcgac agtcgcgata cttcgcccat cctcgcgcga aatgaagcgc
    56581 aattgcacta tctgaaggct attgagagta agcagctgat attcgccacg ggcgaagccg
    56641 ggtgcggaaa aacctggatc agcgcagcaa aagcggcaga ggccctgata cataaggatg
    56701 tcgacaggat tatcgtcacc cgtccagttc tgcaagccga tgaagatctt ggcttcttac
    56761 ctggagatat cgcagaaaag tttgctccct attttcgccc ggtctacgac gtgctggtcc
    56821 ggcgcttagg ggcttccttt atgcagtact gcctgcgacc ggaaataggg aaggtggaaa
    56881 ttgcgccgtt cgcctatatg cgtggacgta cctttgaaaa tgcagtcgtc attcttgacg
    56941 aggcgcagaa tgtgactgcc gcgcaaatga aaatgttttt aacccgcctc ggggagaacg
    57001 tgacggttat cgtcaacggt gatatcacgc aatgcgattt gcctcgcggc gtgtgctccg
    57061 gattaagtga cgcgctggaa cgtttcgaag aagatgaaat ggttgggatc gtccggttcg
    57121 gtaaagagga ctgcgtacgt tcgacacttt gccaacgtac gctgcatgca tacagttaag
    57181 tgtattatcg gtgcagagcc cgggcgaacc gggctttgtt ttgggtgttt atgcccggac
    57241 tagcgctttt tctgaaacaa ccatttttat ttgcccctgg ctggtgaagt gtacgctcat
    57301 cctgtggctt ttttgtagtt gctgatagag ctcatcaggt attgctaagc tctctgcata
    57361 ttcttgcggc gtatattggt aggctgcatg atgctgctgt ttttgaaaac gcagcttatt
    57421 gtacagcgcc cagacaatta acacgacggc attcgccact gccagcaaaa aatagaactg
    57481 aagtcggctt ctggcctcgc tttgccagta ataacccgtc agcagatcca tggcgaataa
    57541 gaatatgaac aacgcaaata atgtccacaa gatggttgtg gcaacataat caaccagtaa
    57601 acgtactggt gattgtcggg tcgtaataat taaattgttc atattaacct ctcagaatcc
    57661 cgcgatcggg acttacccaa cgggcgcgtt gctttttagg catcaacatg actcgtgtaa
    57721 atgataccaa tgtcgttgcc aggctcagca tccagaaaat aaccgggaac caaataatcc
    57781 agaaaagcga tgaagtcaga ttatgctcat agcgattctc gatcatcagg ctgacaataa
    57841 attgcagtaa acataacgta cacaataata ttccggcagt atgtgtcgca gcgatatgtg
    57901 tcaattcaat atttaacggt acaccggcaa gttggactgc gtaaataatg aaaccgacca
    57961 ggcaggtgaa ggcccatatt gtcgtcaggc agtattcaaa aaacagcggc cacattcgaa
    58021 agttttcttt gcgccacaac cttgtcatat ttttgaggaa tacttctgca ccgccctgag
    58081 cccagcgcag gcgctgtttc cacagccctt ttaacgtttc aggcattaat atccagcaca
    58141 gcgcccgtgg ctcgtaaaaa atcgtccact gattcaactg cagcttccag ctaatatcaa
    58201 tatcttcggt gatcatatcg tcactccagt aacccacttc tgccagggcg ctgcgacgaa
    58261 atgcggcaat aacaccggaa acggtaaata cgtttccata gatacgctgg gttcgcttga
    58321 tcaaaccaat aattgaggaa tactcgccaa cctgaatttt acccaccagg gtagaacgtg
    58381 ttcgaatacg aggattaccg gttacggcac ccacacgcgg gttgtacaac atcggttcca
    58441 caatatatgc cgccgcatcg cggtctaata acgcatcgcc atcaatgcac accagatatt
    58501 cacttttcgc cgcggcagct ccggttttaa gcgcaatggc tttcccctgg ttttgcgcca
    58561 gatgaatgac ccgcaaatgg ggaatttgtg cagccatgcg atccaggatg gcacgggttt
    58621 tatctgttga accgtcattt acggcaataa cttcaatgtt ctcataacgc tgtgctaaag
    58681 cggcgtgtat ggtttcctca acgtttttct cctcattaaa acagggaatg ataatggaga
    58741 tagacggatt atctttcaac tggggagctg gtgcgttttc tccccacggc cagtggcgtt
    58801 cacgatagac ccagaaatag acgccgccaa caatccacat aatggacata aaaaacggcc
    58861 agaagaaaac gaacctcatc atcagttcac cagagtgaaa gtacgctacg cataggggta
    58921 tgcataacac cagacataat ataaaaaacg atacgatgcg attaatcatt tttcggatac
    58981 caggctgttg aaaactcagg acgaataagg tctatttcag gttggttatg cagaaaattg
    59041 tcgggataat aaccatagtt tttcactcca ttcagttgta ataggctcat ccagtgagcg
    59101 agttgttgcg aagaaatagc ctgatgctga ccatttttct gccagttttg tgcctgtaat
    59161 tctaaaatag atttgtcttt agcctgaggg atgtttttaa tttgattggt caattgtatt
    59221 aaccattggt cagccgattt ttctgcgaca ccttccagat aaggcatagc cataatagcg
    59281 gtccagtcat agctttttag gaaatcagca taattctgtg caaaccaggc ttcactttca
    59341 ggttgtatta ccggaagtgc aaaaatattt cgtgcagttt taatatgtgg accgcgaatg
    59401 gcttttacgc gcgcactaag ttctaaagtg aagtcagtta acgcacgact tttaaagcgg
    59461 gcccactgtt taaattgctc cgggttttgt cgaatttcgc tcagactccc gctaaagcct
    59521 gcttgctgat aagccgtgat agccggtgca ctggcatctt cataatctga aagcaaagca
    59581 tcatcgtgga acaatatgcc atcaaaagca gcatgtccgg caagatcttc atataacatg
    59641 ccaacttgtg ctctgactct gtcatcgaaa ggagagagac ggtgatattg ttcaggatga
    59701 atttgtgctt ttttctcccc tgttggtaag tattttactc gcgttaatgt gggatctaaa
    59761 tcccagctta ataccggcat ccacgcatag atgtttacac ctgagcgggt acgtaattgc
    59821 caggcaaccc gactaaaaat atctgctttc attggtagca aacgatttgg aaaccagacc
    59881 tctttgacca gcccatcacc atcgggatca gcaaatgcct gcaaatacac ggttgatatt
    59941 tgcatatctt tcacccgctg aattagcaca tcaatattgc gatccatttg ctggaggttt
    60001 tcgtcataaa cgtaatcaag atcgatatgc attatccgtt gtggtgattt ttcctgtacg
    60061 gtaataattt gctgggcaaa ctcttttaat gagggattat tggcgattaa tacccgcgga
    60121 atggaatcca attgcgacgc atttgccaaa cctgattcaa gggtgaagaa catgtcataa
    60181 ccgagttttt ttaattcctc tatcgctatg ccattcgctt cgccataagg ccaaacaaaa
    60241 acgtgtggat ttacctcaac ctttgtacgc aggtattccg tcatttttac agcatccaga
    60301 cgaattcttt cccggtattc tgctgcggtt tcataccgtg cgtggtcagt aaaatatgca
    60361 cgatttacat atacaggcaa taagctgccg gtggcattag cctgaatacc gtagtgagaa
    60421 ttccatgtat gagaagcgag ctcaacgagc cgggaacgcg caacttctcg cacttgttgc
    60481 cacgtggcaa aatattctcg atcgaccaac tcatcgccaa attttacttg tttatccgct
    60541 ggcgtatcga cccaactgcc gacgggggcc catacagcag gccactggaa ggcctgaaga
    60601 attgggaaga cgcgggtata aaaactctgg tagccgtcat caaaagtcag cactacagct
    60661 ttttccggta gcggttttcc tcctcgatgt gcttcacgaa tttgagcaat actgaccggt
    60721 tgataaccgt tctcgcgcag ccaggcaaat tgttcacgca gtgctgatgt ccgcactgac
    60781 ataaaacgct ggtcggcagc ttcgtcttca acgttatgcc atgaaatcgc tacaaaacca
    60841 ttatgcggcc acggttgctc ggcgagtaaa gattcgcgat cctgtggcgg tataaatgat
    60901 gttcttgact ggctaatgca cgcggtgagc ataattatac tcaccagcat caggagatat
    60961 ttatttccat tacgtaacat atttatcctt aaaatctgaa tgtcatatcg aattcaacgt
    61021 ataagttgtg ttctctgtca ccgtcataag gtcgtttttc ccagcgtagc gttgcgccag
    61081 catcaatcac gtcattccag ctaatgcgtt gcccgtagcc gagttgggtg acgacatccg
    61141 tgccataatg tttttgccag gaggcaccaa cacctgcgct gaatatttgc tcccagctat
    61201 tttcatagct tcgccataac aaatggcttg cctcaaatgc cggaacaata tcgaacgttt
    61261 ttatagggtt gtagtatggg gtatcgtgtt ctgtattttg ttcgtaatac agactgggta
    61321 ggaaatcgac aatcaaatat ggtgaagacc agatgcgttc ctgaccctca agtgagactt
    61381 catgacgctg gttactgtcg gaaaaatcag tgaaagccca ggagacaccg tacttacgcc
    61441 gctcattttg ataccagcga acataagcct gagcactgtt gcctgtaaca ccatttttca
    61501 ttgcccgtaa tggaacgcgg tgagagaggc gttccagttg cgaaccaata cgccagttat
    61561 cattaaaatc ataccagcca gacaggcgcg cgccgggttt atgctcatga ttgaaaacgc
    61621 gttcagcgta ctctgcctcg agccagatat tacgtgaccg ccactcaaca cccgcaagcc
    61681 agtcgcgaac aatccctttt ccttcgctaa attgtccatc ggcataaccg aatccagcaa
    61741 aaccgcgcca gttatccttc agcggtggtg aataaacgat ggtggttaag tcgacatcat
    61801 gtttaccact atccgggcct tcggcatcaa ttcctgttga gccagcgata cgaagctctg
    61861 caagattatg tacatcaacc gcacgtttta atcgtacaac gccgggatct tgcggttcac
    61921 gttcgacaac atcgtgcgtt aagacagctg cctgctgcca ttcttgtaac gttaatgctg
    61981 tccaggcttg ttcaacctcc agattaatat tacgtggctc gatcacttct gcttttttta
    62041 attcattttc tgctgcacga ggccaaccgc gggcttgtaa cacactcgcg taatcaatgc
    62101 gcagtccctg atttcctggt gcgttataag caagctctct ggctgtcatt tcagcctgag
    62161 gaagatcatt actatatttt gctacggttg agagaaacga atgcccctgt aaccaggtat
    62221 cattcgggat gctcgtaggc gtgcccatta accgaaggaa aggcggcgaa gtattaatgg
    62281 tatgttgggt gacagttagc gcgcccggat aattttcact ctccaggtgg ctgtaaaaga
    62341 gatccgcaag ttcttcatcg gataaatccg gggcaatggt ctccttgtga taaaagagct
    62401 cggtcattat tgactgtgct tttttcggct gatgatcttt gagataagcc gatgcaaccc
    62461 aatattgccc ccagggcgga ataatttgcc ccgtcttttt taatcgctga tagtgagaaa
    62521 taacgtcttt ataacgatcg cgagttaata acgcgccaag atgatcaacc tgaatacgct
    62581 ggtactgggc agtacggtct gggttatcgt gccacagaat ttctaatgca gcgtattggg
    62641 cgagggcgcg atcggcaatg gcataacgtt cactttcact gcgcgtaggc ataaacgaca
    62701 gtctgaccag ttcggcatga atatcagcgc gaatatctgg cgttaaattg gcatcgtcaa
    62761 tcgcggcagc aagttgatta ttacgtaatg cctgcacgta ttctgtggga tattgttgcg
    62821 tagatgcatt ttcaggtaat gactctgtca tcgcccgtaa ttcatcctga tgacgccccg
    62881 ccagtttata gatataggct tctgcgagta aattggcttt gtccggtgct ccagagttaa
    62941 gctgcttaag tttaaccagc gcagtatcat agtgaccagc atctgccagg gttaaaattt
    63001 gtccccgttg ataatcctta ttttgcggct ccagagagag cgccttttgc cacagtgtaa
    63061 gcgagttttg ccattgttgc aggttacgat aagcgacggc gacagctgca taaccacgcg
    63121 ctggtaattg ctgatgacgg tagcggttgt aaacggtaat aacctgttta tcttgcccgg
    63181 cccataaggc aatctgtaac cagtcagcaa tttggttatt gctgagtgct gattttagtg
    63241 caaaccatga caaagctggc tgagtattac ccttgcgagc ttcaataatc aatgcatcat
    63301 aggcgttatt aacagcactc ttcgccgcgg gactcgctgc taaaaatgcg gcagtaagaa
    63361 gtttcaaagc ccatttggtt ttcgggcacc tttttctgct acttgaatac atcctgtatt
    63421 actccatgta ttgccaaaat ctctctctgt atctaattac aggtaactga aaagaaagat
    63481 atttttgcac ctcataatcc gttattaaac gcggaagaga gacgtgaatt gttgatgatg
    63541 agaagaagaa atgatgagca gagtgtccat ataaaatcct tttctcgccc gaaaatccat
    63601 tccaatgatg aggatcttca ggaatacggc ataaatccca atgccttttt caaaataaat
    63661 taggattaaa ataattaaat cagtaaattc cgatgcatga tttcggattt ttcgaaagtc
    63721 ctgatgaaag gctgcgctac ggcactcacg gatttaattg ttattcctaa tgtatctatg
    63781 aattaatgtt ttataaggat tttctataac catttataag tattttcaag cctggcttgt
    63841 tgcaaacaat gtataaagca cttaggcaat aataattaca ttcagcaact atcatcatcg
    63901 gtattgtttg tgggcggaat ttcatattga caacagtaca gttcttatat ctattaataa
    63961 tagaaaggga tctacaacct acagattggt gtagctttat ggaaaaagac tatttgagaa
    64021 ttagtagtac tgtattagtg agcttattgt ttgggcttgc tttggttttg gtgaatagtt
    64081 ggtttaatca gccaggcgtt gaggaagttg ttccacgatc aacctatctg atggtaatga
    64141 ttgctttatt ttttatcgat actgttgcat ttatttttat gcagttgtat ttcatttatg
    64201 accgtaggca attttcaaac tgtatactta gtttggcttt tctgagttgt ttgatttact
    64261 ttgttaaaac cgtcattatc attcagcaaa ttattgaggg gcgtttgaca agcagtgttg
    64321 tccaaaatga cattgcaatc tattatttgt ttcgtcagat gagtttgtgc atattaatat
    64381 ttctggcatt ggtgaataaa gttagtgaaa acacaaaaca gcgcaattta ttttccaaaa
    64441 aaatgacttt gtgcataagt ttgttttttg tttttggggg gccgatagtt gctcacatac
    64501 tttctagtca ttatgagagc tacaatttac acattgccga gttaaccaat gaaaatggtc
    64561 aggtggtttg gaaagcctca tatgttacca taatgatttt catgtggtta accttgcttt
    64621 cggttaatct atactttaat ggtttacgct atgatatctg gaatggagtg acggtgattg
    64681 ctttttgtgc cgtactgtac aatatctctc tgttatttat gagtaggtat agcgtgtcca
    64741 cttggtatat tagtcgcact attgaagtag tcagtaagtt aactgttatg gtaatattta
    64801 tgtgccatat attcagtgcg ctacgagtaa caaagaacat tgcacatcgc gatcccttaa
    64861 ccaatatatt taacagaaat tattttttta atgaactgac agttcaatca gcatcagcca
    64921 aaaaaacgcc ttattgcgtc atgattatgg atatcgacca cttcaaaaaa gtcaacgaca
    64981 cctgggggca tccggttggc gatcaggtga taaaaacagt ggtgaatatc attgggaaaa
    65041 gcatacgacc agatgacctt ttagcgcgcg tcggcggcga agagtttggc gtcttgctga
    65101 cggacatcga tactgaacgt gcgaaagctt tagcggaaag gattcgggaa aatgttgagc
    65161 gtttaactgg cgataatcct gaatacgcta taccacaaaa agtgacgatt agtattggcg
    65221 ctgttgtgac tcaggaaaat acgttaaacc caaatgagat ctatcgactg gctgataacg
    65281 cactttacga ggcgaaagag accgggcgta ataaggtggt tgtgagggat gtggtgaatt
    65341 tttgtgagtc accataaagc ggcat
//
LOCUS       DAFATA010000013        64539 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFATA010000013 DAFATA010000000
VERSION     DAFATA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.013"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..599)
                     /gene="rcsB"
                     /locus_tag="LGJ20_001196"
     CDS             complement(<1..599)
                     /gene="rcsB"
                     /locus_tag="LGJ20_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBK2385292.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIA"
     gene            complement(616..3288)
                     /gene="rcsD"
                     /locus_tag="LGJ20_001197"
     CDS             complement(616..3288)
                     /gene="rcsD"
                     /locus_tag="LGJ20_001197"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBK2385293.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDNWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEIASALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            4027..5151
                     /gene="ompC"
                     /locus_tag="LGJ20_001198"
     CDS             4027..5151
                     /gene="ompC"
                     /locus_tag="LGJ20_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBK2385294.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGYATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNFGLNGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGW
                     ANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVVAGRNYDDEDILKYVDVGATYYF
                     NKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            5263..6318
                     /gene="apbE"
                     /locus_tag="LGJ20_001199"
     CDS             5263..6318
                     /gene="apbE"
                     /locus_tag="LGJ20_001199"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBK2385295.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            6392..7456
                     /gene="ada"
                     /locus_tag="LGJ20_001200"
     CDS             6392..7456
                     /gene="ada"
                     /locus_tag="LGJ20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBK2385296.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            7456..8106
                     /gene="alkB"
                     /locus_tag="LGJ20_001201"
     CDS             7456..8106
                     /gene="alkB"
                     /locus_tag="LGJ20_001201"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBK2385297.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            8182..9825
                     /gene="yojI"
                     /locus_tag="LGJ20_001202"
     CDS             8182..9825
                     /gene="yojI"
                     /locus_tag="LGJ20_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBK2385298.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            10043..11689
                     /gene="mqo"
                     /locus_tag="LGJ20_001203"
     CDS             10043..11689
                     /gene="mqo"
                     /locus_tag="LGJ20_001203"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBK2385299.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(11838..12326)
                     /gene="eco"
                     /locus_tag="LGJ20_001204"
     CDS             complement(11838..12326)
                     /gene="eco"
                     /locus_tag="LGJ20_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBK2385300.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(12461..12625)
                     /locus_tag="LGJ20_001205"
     CDS             complement(12461..12625)
                     /locus_tag="LGJ20_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385301.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            12734..13228
                     /gene="napF"
                     /locus_tag="LGJ20_001206"
     CDS             12734..13228
                     /gene="napF"
                     /locus_tag="LGJ20_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBK2385302.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            13218..13481
                     /gene="napD"
                     /locus_tag="LGJ20_001207"
     CDS             13218..13481
                     /gene="napD"
                     /locus_tag="LGJ20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBK2385303.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            13478..15964
                     /gene="napA"
                     /locus_tag="LGJ20_001208"
     CDS             13478..15964
                     /gene="napA"
                     /locus_tag="LGJ20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HBK2385304.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            15971..16666
                     /gene="napG"
                     /locus_tag="LGJ20_001209"
     CDS             15971..16666
                     /gene="napG"
                     /locus_tag="LGJ20_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBK2385305.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            16653..17516
                     /gene="napH"
                     /locus_tag="LGJ20_001210"
     CDS             16653..17516
                     /gene="napH"
                     /locus_tag="LGJ20_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBK2385306.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            17513..17962
                     /gene="napB"
                     /locus_tag="LGJ20_001211"
     CDS             17513..17962
                     /gene="napB"
                     /locus_tag="LGJ20_001211"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBK2385307.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            17972..18574
                     /gene="napC"
                     /locus_tag="LGJ20_001212"
     CDS             17972..18574
                     /gene="napC"
                     /locus_tag="LGJ20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBK2385308.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            18593..19210
                     /gene="ccmA"
                     /locus_tag="LGJ20_001213"
     CDS             18593..19210
                     /gene="ccmA"
                     /locus_tag="LGJ20_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBK2385309.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            19207..19869
                     /gene="ccmB"
                     /locus_tag="LGJ20_001214"
     CDS             19207..19869
                     /gene="ccmB"
                     /locus_tag="LGJ20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBK2385310.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            19911..20648
                     /gene="ccmC"
                     /locus_tag="LGJ20_001215"
     CDS             19911..20648
                     /gene="ccmC"
                     /locus_tag="LGJ20_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBK2385311.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            20645..20854
                     /gene="ccmD"
                     /locus_tag="LGJ20_001216"
     CDS             20645..20854
                     /gene="ccmD"
                     /locus_tag="LGJ20_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBK2385312.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            20851..21330
                     /gene="ccmE"
                     /locus_tag="LGJ20_001217"
     CDS             20851..21330
                     /gene="ccmE"
                     /locus_tag="LGJ20_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBK2385313.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            21327..23270
                     /gene="ccmF"
                     /locus_tag="LGJ20_001218"
     CDS             21327..23270
                     /gene="ccmF"
                     /locus_tag="LGJ20_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBK2385314.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            23267..23824
                     /gene="dsbE"
                     /locus_tag="LGJ20_001219"
     CDS             23267..23824
                     /gene="dsbE"
                     /locus_tag="LGJ20_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBK2385315.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            23821..24873
                     /gene="ccmH"
                     /locus_tag="LGJ20_001220"
     CDS             23821..24873
                     /gene="ccmH"
                     /locus_tag="LGJ20_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBK2385316.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSLQESK"
     gene            complement(24908..25555)
                     /gene="narP"
                     /locus_tag="LGJ20_001221"
     CDS             complement(24908..25555)
                     /gene="narP"
                     /locus_tag="LGJ20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBK2385317.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            25875..28466
                     /gene="yejO"
                     /locus_tag="LGJ20_001222"
                     /pseudo
     CDS             25875..28466
                     /gene="yejO"
                     /locus_tag="LGJ20_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(28570..28646)
                     /locus_tag="LGJ20_001223"
     tRNA            complement(28570..28646)
                     /locus_tag="LGJ20_001223"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28610..28612),aa:Pro,seq:ggg)
     gene            complement(28721..30481)
                     /gene="pbgA"
                     /locus_tag="LGJ20_001224"
     CDS             complement(28721..30481)
                     /gene="pbgA"
                     /locus_tag="LGJ20_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBK2385318.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(30501..30728)
                     /locus_tag="LGJ20_001225"
     CDS             complement(30501..30728)
                     /locus_tag="LGJ20_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBK2385319.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            30910..31917
                     /gene="yejK"
                     /locus_tag="LGJ20_001226"
     CDS             30910..31917
                     /gene="yejK"
                     /locus_tag="LGJ20_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBK2385320.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(32056..32340)
                     /gene="rplY"
                     /locus_tag="LGJ20_001227"
     CDS             complement(32056..32340)
                     /gene="rplY"
                     /locus_tag="LGJ20_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBK2385321.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(32465..34225)
                     /locus_tag="LGJ20_001228"
     CDS             complement(32465..34225)
                     /locus_tag="LGJ20_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK2385322.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            34374..35069
                     /gene="rsuA"
                     /locus_tag="LGJ20_001229"
     CDS             34374..35069
                     /gene="rsuA"
                     /locus_tag="LGJ20_001229"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBK2385323.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            35097..36287
                     /gene="bcr"
                     /locus_tag="LGJ20_001230"
     CDS             35097..36287
                     /gene="bcr"
                     /locus_tag="LGJ20_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBK2385324.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            36620..36964
                     /locus_tag="LGJ20_001231"
     CDS             36620..36964
                     /locus_tag="LGJ20_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385325.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(36968..38557)
                     /gene="yejF"
                     /locus_tag="LGJ20_001232"
     CDS             complement(36968..38557)
                     /gene="yejF"
                     /locus_tag="LGJ20_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBK2385326.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(38559..39584)
                     /locus_tag="LGJ20_001233"
     CDS             complement(38559..39584)
                     /locus_tag="LGJ20_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2385327.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(39584..40678)
                     /locus_tag="LGJ20_001234"
     CDS             complement(39584..40678)
                     /locus_tag="LGJ20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBK2385328.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(40679..42493)
                     /locus_tag="LGJ20_001235"
     CDS             complement(40679..42493)
                     /locus_tag="LGJ20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2385329.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFRKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKASKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(42575..44131)
                     /locus_tag="LGJ20_001236"
     CDS             complement(42575..44131)
                     /locus_tag="LGJ20_001236"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBK2385330.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(44312..44878)
                     /gene="mepS"
                     /locus_tag="LGJ20_001237"
     CDS             complement(44312..44878)
                     /gene="mepS"
                     /locus_tag="LGJ20_001237"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBK2385331.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRVVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(45290..46003)
                     /gene="lpxT"
                     /locus_tag="LGJ20_001238"
     CDS             complement(45290..46003)
                     /gene="lpxT"
                     /locus_tag="LGJ20_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBK2385332.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(46042..47028)
                     /gene="yeiR"
                     /locus_tag="LGJ20_001239"
     CDS             complement(46042..47028)
                     /gene="yeiR"
                     /locus_tag="LGJ20_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBK2385333.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(47146..48612)
                     /locus_tag="LGJ20_001240"
     CDS             complement(47146..48612)
                     /locus_tag="LGJ20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK2385334.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(48835..49407)
                     /gene="yeiP"
                     /locus_tag="LGJ20_001241"
     CDS             complement(48835..49407)
                     /gene="yeiP"
                     /locus_tag="LGJ20_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBK2385335.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            49562..49816
                     /locus_tag="LGJ20_001242"
     CDS             49562..49816
                     /locus_tag="LGJ20_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBK2385336.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(49813..50994)
                     /gene="setB"
                     /locus_tag="LGJ20_001243"
     CDS             complement(49813..50994)
                     /gene="setB"
                     /locus_tag="LGJ20_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBK2385337.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            51362..52492
                     /gene="fruB"
                     /locus_tag="LGJ20_001244"
     CDS             51362..52492
                     /gene="fruB"
                     /locus_tag="LGJ20_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBK2385338.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            52492..53430
                     /gene="fruK"
                     /locus_tag="LGJ20_001245"
     CDS             52492..53430
                     /gene="fruK"
                     /locus_tag="LGJ20_001245"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK2385339.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            53447..55138
                     /gene="fruA"
                     /locus_tag="LGJ20_001246"
     CDS             53447..55138
                     /gene="fruA"
                     /locus_tag="LGJ20_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBK2385340.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            55562..56503
                     /locus_tag="LGJ20_001247"
     CDS             55562..56503
                     /locus_tag="LGJ20_001247"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBK2385341.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            56491..57429
                     /gene="psuG"
                     /locus_tag="LGJ20_001248"
     CDS             56491..57429
                     /gene="psuG"
                     /locus_tag="LGJ20_001248"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBK2385342.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            57523..58773
                     /locus_tag="LGJ20_001249"
     CDS             57523..58773
                     /locus_tag="LGJ20_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK2385343.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(58844..59542)
                     /gene="yeiL"
                     /locus_tag="LGJ20_001250"
     CDS             complement(58844..59542)
                     /gene="yeiL"
                     /locus_tag="LGJ20_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBK2385344.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            59672..60613
                     /gene="rihB"
                     /locus_tag="LGJ20_001251"
     CDS             59672..60613
                     /gene="rihB"
                     /locus_tag="LGJ20_001251"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBK2385345.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            60713..61963
                     /locus_tag="LGJ20_001252"
     CDS             60713..61963
                     /locus_tag="LGJ20_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK2385346.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(62019..63107)
                     /locus_tag="LGJ20_001253"
     CDS             complement(62019..63107)
                     /locus_tag="LGJ20_001253"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK2385347.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(63111..63968)
                     /gene="nfo"
                     /locus_tag="LGJ20_001254"
     CDS             complement(63111..63968)
                     /gene="nfo"
                     /locus_tag="LGJ20_001254"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK2385348.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(64042..>64539)
                     /locus_tag="LGJ20_001255"
     CDS             complement(64042..>64539)
                     /locus_tag="LGJ20_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HBK2385349.1"
                     /translation="YPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAISPDAENAAVI
                     SKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAIFNSFHLLPQS
                     VVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLIVGGGAINYVI
                     QSVIA"
BASE COUNT        15555 a        15800 c        17293 g        15891 t
ORIGIN      
        1 gcgatatcgt tctcgacacc cagcttcatc atcgcagatt tcttctggct actgatggtt
       61 ttaatactgc ggttcagctt tttagcgatc tcggtcacca ggaagccttc cgcaaacagg
      121 cgcagaactt cactctcttt tggcgagaga cgcttgtcac cgtaaccacc agcactgatt
      181 ttttccaaca ggcgagaaac gctttccggg gtaaatttct tccctttctg cagcgcggcg
      241 agagctttcg gcagatcggt cggtgcacct tgtttcagca cgatcccttc gatatccaga
      301 tccaataccg cactaagaat cgccgggttg ttgttcatgg tcagaacaat gatcgacagg
      361 cttgggaaat ggcgcttgat gtacttgatt aaggtaatgc catcgccgta cttatcgcca
      421 ggcatggaga gatcggtaat caacacatgc gcatccagtt tcggcaggtt gttgatcagt
      481 gctgtagagt cttcaaattc gccgacaaca ttcacccact caatttgctc aagtgatttg
      541 cgaataccga acaagactat cggatggtca tcggcaataa ttacgttcat attgttcatg
      601 taataggcta ccttgctaca gcaagctctt gacataactg tcaatgtcgc tgatgtattt
      661 ttctattcct ggaacatcct tctcacgaat cagatgttcc agcgtttcac ataactgctt
      721 gccgggtacc agatttagca tggcaaatac gcctttaaga cgatgagccg tttgggctaa
      781 cgcagcaaag tcactggttg ctgcttcagt atacagcctc ttaacatcat ccggtactgt
      841 gtctacaaag agcgcataat agccgctggc atggagttgc gcattttcat ctccgcccag
      901 aggcgattct gtcacctctt cctgcgccag ttgcacttca attaattgta agaccgcttc
      961 ctgcatagcg ttgctcatat tgaagttgac gcacaattga ccaggcccaa tttcccgtac
     1021 gccagactca tcatcgctta aaagcaagcc agaggcagta agattagacg gattatccgt
     1081 taaaaagata tcataatctt gactaattaa tctttcatcg ggtgtgatac aggttgcacc
     1141 ccaattttct aactggcgag tgacaatatt ccgaatttct gccgaagtaa catccaccat
     1201 tacgcagaca tcatccagta aacgctcttc ttcctcttca acttccgggt cagctgcgag
     1261 cattttgata tgcacagagt agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg
     1321 accgcccagt ttacgtgcca gttgatcgct cagccagaat gccagcgggt ccgccttgcc
     1381 atagcgatcg ttttgggtct ggttgataaa cgggaagtgc aaattatcca tttcatgaat
     1441 acttacgcct tctcccgtgt ccagaatgcg gaacgtcagg cggtcttcgg aggactcatc
     1501 ctgatcaacc tcaagggtga tttttcccaa ttgcgttgag gtcacggcat attgcatcag
     1561 tagcagcaaa atacgtcgta aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag
     1621 atgattgtta atcagcagtt gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac
     1681 aacttcatca attaaatcct gcacggagaa cagcaccgtc tcacttttcc agctatcatc
     1741 cgcaagcatg ttcgctaact gtatttcatc aaccagccgc accagcacat ctgcctgatt
     1801 cgccagttgt ttgctttccg gggcgttgag tttagccgcg ctctccgcca gggactgtgc
     1861 gggttctttc agcgcatcgc caatgttttt cataaaggtc atccgcccct gctggttttt
     1921 ctcatacaga cgctgcgcct gcttgagttt cttgtttacc agcacttcgc gatcctgatc
     1981 gcgaataatg aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc ggatctcata
     2041 cagctcgtta ttgatcgtcg cctgaataat cccctgatgc tgttccgcca tggtggtgat
     2101 gttttgcaga ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta tgacagtgcg
     2161 gttcgattcc tgatcgtgaa ccagcaggcc gagcggcagc agtgagacta tctcttcatt
     2221 aattgcccgt aaaatgcgca attcgttatt gaccgccgtg ctgggtagac tttctgtact
     2281 gcggctggag aaatggcgga atgtggtata gccaaataac gccagcgcca gcaaaccgat
     2341 gttcagcagc agtggcagca gaatgttttg caacgtatcc agcaataagg tgccataagg
     2401 aacctgccag accaggcgca tatcggtaga gttgagtgcc gaggcgattt caatcttcgt
     2461 actgttaaag tggatactga cgctatccgt tccttctttc tcattatcat tgtctcccgt
     2521 tgccgtcgtg tctggctcaa ggcggaaact gtccagcggc atacccggtg ggatcaaatc
     2581 attaatcggc agatcaaaag ccacgaccgt tgccagatgt cctggctggt taaaggtagt
     2641 acgcaatgta aagtaatgac cgttctgcca ggccaggcgg cgcatgttag aaaagctttc
     2701 gcgttcatcg agggcgttgg cctgttgcaa catctctgca cgacgtgaat caacaatgtc
     2761 gctgacggtc gattctttaa atccggaggt gagatcttta aggggtaggg ttgagatcag
     2821 caccagactg ttatcctgac cattcaggta atacatcgac cacggtacat tttctgcgcc
     2881 ccacaatgta tccagataag tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg
     2941 agagccaaag attaacgctt ccgttttgcg gcgtggtttc tccagatagt agacatcctg
     3001 tttcaggcgc gtctcttgta aaccttcgcc ggaggagggg gaggtcgtcg cggcaatgtt
     3061 gtcgtagatc tgccaggtca cgtaacgcca gttatcgacg cgcttttgga tagcatgggt
     3121 aatgtcgaca atctggtaac ttttatcttt cagccaggcg ttaacggcgc tttgtaccat
     3181 tacacccatc gtcaccagta acacaatgat caacagtaaa aagaagcggg taatgctccc
     3241 cggtaggagt gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc gtgtttatga
     3301 cctgttaaaa cttcgcgaag tgatagggca gtataaaggg tacagtgtga aattccagac
     3361 tcttacctga ttgatgattc tctattctcc cgtgatattt atcatcctgc attgggggaa
     3421 atagatgtac aagcgccatt ttgctgtaca aataacctac aaaaagccca acaaaaataa
     3481 ttgcaaataa acaaagattg ctggaaatta tgcggatgtt aattatttgt gaaatagtta
     3541 acaagcgtta tagtttttct gtggtagcac agaataatga aaagtgtgta aagaagggta
     3601 aaaaaaaccg aatgcgaggc atccggttga aataggggta aacagacatt cagaaatgaa
     3661 tgacggtaat aaataaagtt aatgatgata gcgggagtta ttctagttgc gagtgaaggt
     3721 tttgttttga cattcagtgc tgtcaaatac ttaagaataa gttattgatt ttaaccttga
     3781 attattattg cttgatgtta ggtgcttatt tcgccattcc gcaataatct taaaaagttc
     3841 ccttgcattt acattttgaa acatctatag cgataaatga aacatcttaa aagttttagt
     3901 atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttgcc gactgattaa
     3961 tgagggttaa tcagtatgca gtggcataaa aaagcaaata aaggcatata acagagggtt
     4021 aataacatga aagttaaagt actgtccctc ctggtcccag ctctgctggt agcaggcgca
     4081 gcaaacgctg ctgaagttta caacaaagac ggcaacaaat tagatctgta cggtaaagta
     4141 gacggcctgc actatttctc tgacaacaag tcagaagacg gcgaccagac ctatgtacgt
     4201 cttggtttca aaggcgaaac tcaggttact gaccagctga ccggttacgg ccagtgggaa
     4261 tatcagatcc agggcaatac ctctgaagac aacaaagaaa actcctggac ccgtgtggca
     4321 ttcgcaggtc tgaaattcca ggatgttggt tctttcgact acggtcgtaa ctacggcgtt
     4381 gtttacgacg taacttcctg gaccgacgta ctgccagaat tcggtggcga cacttacggc
     4441 tctgacaact tcatgcagca gcgtggtaat ggctacgcaa cctaccgtaa caccgacttc
     4501 ttcggtctgg ttgacggtct gaactttgct gttcagtacc agggcaaaaa tggtagcgta
     4561 agcggcgaag gcatgaccaa caatggtcgt ggtgctctgc gtcagaatgg cgacggtgtc
     4621 ggcggatcta tcacttatga ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc
     4681 aaacgtactg atgatcagaa cttcggcctg aacggttatg gtgagcgtta tttgggtaac
     4741 ggcgaccgcg ctgaaaccta cactggtggt ctgaaatacg acgctaacaa catctacctg
     4801 gctgctcagt acacccagac ctacaacgca actcgcgtag gttccctggg ttgggcgaac
     4861 aaagcacaga acttcgaagc tgttgctcag taccagttcg acttcggtct gcgtccatct
     4921 gtagcatacc tgcagtctaa aggtaaaaac ctgggtgtcg ttgctggtcg taactacgac
     4981 gacgaagata tcctgaaata tgttgatgtt ggtgcgacct actacttcaa caaaaacatg
     5041 tccacctacg ttgactacaa aatcaacctg ctggacgaca accagttcac tcgtgacgct
     5101 ggcatcaaca ctgataacat cgtagctctg ggtctggttt accagttcta atctcgattg
     5161 atatcgaaca aagggcctgc gggccctttt ttcattgttt tcagcgtaca aactcatttt
     5221 tttggtgtac tcttgcgacc gttcgcatga ggataatcac gtatggaaat aagctttacc
     5281 cgcgtggcac tgctggctgc cgcgctcttc tttgttggtt gcgatcaaaa accacaaccc
     5341 gccaaaaccc acgctactga agttaccgtt cttgaaggca aaaccatggg taccttctgg
     5401 cgtgccagca tcccgggcat tgatgccaaa cgcagtgccg aacttaaaga aaagattcag
     5461 acccagctgg acgccgacga tcagctgctt tcgacctata aaaaagattc cgcgctgatg
     5521 cgctttaacg actcgcaaag tttgtcgccg tggccagtaa gtgaagcgat ggccgaaatc
     5581 gtcactacct cgctgcgcat tggcgcgaag accgatggtg cgatggatat taccgtcggg
     5641 ccgctggtga atctgtgggg ctttggcccg gaacaacagc cggttcaaat tccgagccag
     5701 gaacagatcg atgcgatgaa agccaaaacc ggcttacagc acctgacggt catcaatcag
     5761 tcgcatcagc aatatctgca aaaagacctg ccggatttat atatcgatct ctccaccgtc
     5821 ggtgaaggtt atgcggcgga tcacctggca cgcttgatgg agcaggaagg tatttcacgt
     5881 tatctggtgt cagttggtgg tgctctggat agccgtggta tgaacggtga aggccagccg
     5941 tggcgggtag cgattcaaaa accaaccgat aaagaaaacg cggttcaggc cgtggtggat
     6001 atcaacggtc atggcattag cacctctggc agctaccgta actattacga actggacggc
     6061 aaacgtcttt ctcatgttat cgatccgcaa accgggcgtc ctatcgaaca caatctggta
     6121 tccgtgacgg tgattgcccc gacggcgctg gaagccgatg cctgggatac tggcttaatg
     6181 gtactcgggc cggagaaagc caaagaggtt gttcgccggg aagggctggc ggtctatatg
     6241 atcaccaaag aaggtgatag ctttaaaacc tggatgtcgc cgcagtttaa aagcttcctt
     6301 atcagcgaaa aaaattaaag cgcaagattg ttggtttttg cgtgatggtg accgggcaga
     6361 ctaaaggcta tccttaacca gggagctgat tatgaaaaaa gccacatgct taactgacga
     6421 tcaacgctgg caatctgtct tagcccgcga cccgaatgcc gacggcgaat tcgttttcgc
     6481 cgtgcgtacc acaggcatct tttgccgtcc gtcttgccgc gccagacatg ctttgcggga
     6541 aaacgtctcc ttctacgcaa atgccagcga ggcactcgcc gctggctttc gcccctgcaa
     6601 acgttgtcag ccagacaaag ccaatgccca gcaacatcgg ttggataaaa ttacccacgc
     6661 ctgtcgactg ctggaacagg aaacgcctgt aacgctggaa gccttagccg accatgtggc
     6721 gatgagtccg ttccatctgc atcggttgtt taaagcgact accggaatga cgcctaaagc
     6781 ctggcaacag gcctggcgcg ctcgccgttt gcgcgaatcg ctggcgaaag gggagagcgt
     6841 gacgacgtct attcttaacg ccggattccc cgacagcagc agttactacc gcaaagccga
     6901 cgaaacgctg ggcatgacgg ctaaacaatt ccgtcacggt ggcgaaaatc tggcggtgcg
     6961 ttacgcgctg gctgattgtg agctgggtcg ttgcctggtg gcagaaagcg agcgggggat
     7021 ttgcgcgata ttgctgggcg atgatgacgc gacactaatc agcgagttgc agcagatgtt
     7081 tcccgctgcc gacaacgcgc ctgccgatct gatgtttcag caacatgtgc gtgaagtgat
     7141 cgccagcctc aatcaacgcg atacgccgct gacgttaccg ctggacattc gcggcactgc
     7201 ttttcagcaa caagtctggc aggcactgcg cacgatacct tgcggtgaaa ccgtcagtta
     7261 tcagcaactg gctaacgcca tcggcaaacc gaaagcggta cgggccgttg ccagcgcctg
     7321 tgccgccaac aagctggcta tcataatacc ctgtcatcgg gtggtccgtg gtgatggcac
     7381 actttccggt taccgctggg gcgtgtcgcg taaagcgcaa ctgctgcgcc gcgaagctga
     7441 aaatgaggag aggtaatgtt ggatctgttt gccgatgctg aaccgtggca agagccactg
     7501 gcggctggag cggtaatttt gcaccgtttt gcttttaacg ctgcggagca actgatccgc
     7561 gatattaatg acgttgccag ccagtcgccg tttcgccaga tggtcacccc cgggggatat
     7621 accatgtcgg tggcgatgac caactgtggg catctgggct ggacgaccca tcggcaaggt
     7681 tatctctatt cgcccattga tccgcaaaca aataaaccgt ggcccgccat gccacaaagc
     7741 tttcatgatt tatgtcaacg tgcggctacg gcggcgggct atccggattt ccagccagat
     7801 gcttgtctta tcaaccgcta cgctcctggc gcgaaactgt cgctgcatca ggataaagac
     7861 gaaccggatc tgcgcgcgcc gattgtttct gtttctcttg gcttacccgc gatttttcaa
     7921 tttggtggcc tgaaacgtaa tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg
     7981 gtggtatggg gcggtgaatc gcggctgttt tatcacggca ttcaaccgct gaaagcgggg
     8041 tttcatccac tcaccggcga ctgccgctac aacctgacat tccgtcaggc aggtaaaaaa
     8101 gaataaaaat aagaattatt attgctgtgc gcgcgaagat gtttaaactg cgggctgtaa
     8161 ttcattgtcc gggttttctg catggaactt cttgtacttg tctggcggca gtatcgctgg
     8221 ccatttatca gtgtgatggc gctaagcctc gccagtgcgg cattaggcat tggcttaatt
     8281 gcttttatca atcagcgcct tatcgaaacg gcggatacca gtctgctggt attgccggag
     8341 tttctgggat tattgctgct gttgatggca gtcactctcg gatcgcaact ggcgctcacc
     8401 actttggggc atcacttcgt ttaccgactg cgtagtgaat ttatcaagcg gattctggat
     8461 acccacgtcg agcgcattga acaactcggt agcgcctcgt tgctggcggg attaaccagc
     8521 gatgtgcgca atatcaccat tgcttttgtg cgtcttccgg aactggtgca ggggatcatt
     8581 ctcactatcg gttccgcggc gtatctgtgg atgctgtcgg gcaaaatgtt gctggtaacg
     8641 gctatctgga tggcgatcac catctggggt ggttttgtac tggtggcgcg ggtgtacaaa
     8701 catatggcga ccctgcgtga aaccgaagac aagctgtaca cggattttca aaccgtactc
     8761 gaagggcgca aagagctgac tctgaaccgg gaacgcgccg agtatgtgtt taacaacctc
     8821 tacatccccg atgcgcaaga gtatcgccat catattattc gcgcagacac cttccatctt
     8881 agcgccgtga actggtcaaa catcatgatg ctgggcgcta tcggcctggt gttctggatg
     8941 gcgaacagtc tcggttgggc tgataccaac gttgctgcga cctattcgtt gacgctgtta
     9001 ttcctgcgta cgccgctgct ttcggcggtt ggcgcattgc cgacgctgct gacggcgcag
     9061 gtggcgttta acaagctgaa caaattcgcg ctcgcgcctt tcaaagctga gtttccgcgc
     9121 ccgcaggctt ttcccaactg gcaaacgctg gagctgcgta acgtgacgtt tagctatcag
     9181 gataacgcgt tttccgttgg gccgattaac ctcaccatta aacgtggcga gctgctgttt
     9241 ctgattggcg gcaacggtag cggaaaatcg acgctggcga tgttgttgac gggcttgtat
     9301 cagccacaaa gcggcgaaat cttgctggat ggcaaacctg tcagcggcga acaaccggaa
     9361 gattatcgca aactgttttc ggcagtgttt accgatgtct ggctgtttga tcaactgctg
     9421 gggccggagg gtaaacccgc taacccgcaa ctggttgaga agtggctggc gcagctgaaa
     9481 atggctcata agcttgagtt aagcaacggg cgtattgtta acctgaagtt atcaaaaggg
     9541 cagaaaaaac gcgtggcgct gttgctggcg ctggcagaag aacgcgatat tatcctgctg
     9601 gatgaatggg cggcggatca ggatccacac ttccgtcgtg agttttatca ggtgttgctg
     9661 ccgctgatgc aggagatggg taaaactatt ttcgctatca gtcatgatga tcattacttt
     9721 atccacgccg accgcctgct ggaaatgcgc aatgggcaac ttagcgagct gacgggcgaa
     9781 gagcgcgatg ccgcttcgcg tgatgccgtt gcccggacgg cataatatta cgacgctgac
     9841 ctggcggact gctgccgtca ggtcaatatg ctaaaaatcc cttcatttct aataacccta
     9901 taattaatca acgaaattat aatgtttcta aaattagaat ataatttata aacattattt
     9961 aaatgttgtt acttaagtgt taccgttgat gccgcgcaaa tctcacctgc aataaagcga
    10021 ctaaaagtaa ggcattaaca agatgaaaaa agtgactgcc atgctcttct cgatggccgt
    10081 ggggcttaat gccgtttcga tggcggcaaa agcgaaagcg tccgaggagc aggaaactga
    10141 tgtactgttg attggcggcg gcattatgag cgccacgttg gggacctatt tacgcgagct
    10201 ggagcctgaa tggtcgatga ccatggtgga gcgcctggag ggtgtcgcgc aggagagttc
    10261 gaacggctgg aataacgccg gaaccgggca ttctgcactg atggaactga actacacccc
    10321 gcaaaacgcc gatggcagca tcagtattga aaaagcagtc gccattaacg aagcatttca
    10381 gatttcccgc cagttctggg cgcaccaggt tgagcgcggc gtgctgcgta ctccgcgttc
    10441 atttatcaat accgttccgc atatgagctt tgtctggggc gaggataacg ttaatttcct
    10501 gcgcgcccgt tacgccgcgt tgcaacaaag ctcgctgttt cgcggtatgc gttactctga
    10561 agatcacgcg cagatcaaag agtgggcacc gttagtgatg gaagggcgcg atccacaaca
    10621 gaaagtagcg gcaacacgca cagaaattgg cactgatgtg aactatggcg agatcacccg
    10681 ccagttaatt gcttccttac agaagaaatc taacttctcg ctgcaactca gcagcgaagt
    10741 ccgcgcccta aagcgtaatg acgataacac ctggaccgtt accgttgccg atctgaaaaa
    10801 cggcactgca cagaacatcc gcgccaaatt tgtctttatc ggcgcgggcg gtgcggcgct
    10861 gaagctatta caggaatcgg ggattccgga agcgaaagac tacgccggtt tcccggtggg
    10921 cggacagttc cttgtttcgg aaaacccgga cgtggttaat caccatctgg cgaaggttta
    10981 cggtaaagca tccgttggcg caccaccgat gtcggttccg catatcgata cccgcgttct
    11041 ggacggtaaa cgcgtagtgc tgtttgggcc atttgccacc ttctcaacca aattcctcaa
    11101 aaacggttca ttgtgggatc tgatgagttc caccaccacc tctaacgtga tgccgatgat
    11161 gcacgtcggg ctggataatt tcgatctggt gaaatatctg gtgagtcagg tgatgttgag
    11221 tgaagaggat cgttttgaag cgttgaaaga gtactatccg caggcaaaaa aagaggactg
    11281 gcgtttgtgg caagcggggc agcgcgtgca gattatcaag cgtgatgccg agaaaggtgg
    11341 cgtactgcgt ctgggtactg aagtggtcag tgaccagcaa gggaccattg ccgcgctgct
    11401 gggggcatcg ccaggggcgt caaccgccgc gccgattatg ttggatctgc tggaaaaagt
    11461 atttggcgat cgcgtttcca gcccgcaatg gcaggctacg ttgaaagcga tcgttccgtc
    11521 ttatggacgc aagctgaacg gtgatgtagc ggcaacagaa cgcgagttgc agtacaccag
    11581 cgaagtgctg gggctgaaat acgacaagcc gcaggcagca gatagtacgc caaaaccgca
    11641 gttgaaaccg caacccgttc aaaaagaagt ggcggatatt gcgttgtaat gatgtgccac
    11701 atccggcatg gtatgccgga tgtggcgtat gctgataaga cgcgccagcg tcgcatcagg
    11761 caccggctcg ggcgttagat gtcggatgcg gcgtgaccgc cttattcggc ctacggggtg
    11821 ccgcacttca cagcatttta gcgaactacc gcgttgtcaa ttttctcttc cgccttccag
    11881 acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag cttgctgttg
    11941 taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc tttcttgcca
    12001 tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac ataataatca
    12061 tagccccagc cttccagcgt tttgctttcc agcttcccgc cgagacgatg caaattgcaa
    12121 tcgacttcca gcgtctgacc gattaacagt tctactttca gggtagattc atcttcttgc
    12181 ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata aggcgcgatt
    12241 ttttccagtg gctggacgct ttctgccgcc caagcggaag tggtagcgaa agcggcaaac
    12301 aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta taggttttca
    12361 ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct ttatgaataa
    12421 cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg atgagggagc
    12481 aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta tagccatttg
    12541 aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat cctgctacag
    12601 gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta gcggctataa
    12661 aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag cgcgttaatg
    12721 atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg gtcgctggcg
    12781 caaagccagt aacggtatcc gtccaccctg gtccggtgat gaatctcatt ttctgaccca
    12841 ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc aacgcggcgc
    12901 gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt acgcctgcgc
    12961 gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg atttgcagtt
    13021 caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct gtcaggatag
    13081 ctgtgagcca atggcaatta tctttcgccc gacgctctcc ggaatctacc agccgcaact
    13141 taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg tatcagccat
    13201 aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc gtgcaggcca
    13261 aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc tgtgaagttg
    13321 ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa gacagcgaaa
    13381 cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg gtgtcgctgg
    13441 tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc gtcgtagctt
    13501 tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg tgccgggcgt
    13561 tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc cgtgccgttt
    13621 ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg tggcctgtca
    13681 gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct atttcctgcc
    13741 caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga aaaacggtaa
    13801 atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg atgtgatgga
    13861 agagaaattc aaaaccgccc tgaaagaaaa aggaccggaa tcgatcggta tgttcggttc
    13921 tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgttcaaag cgggcttccg
    13981 ttcgaacaac atcgacccga acgcacgtca ctgtatggcg tcggcagtag ttggctttat
    14041 gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc aggctgacgc
    14101 gtttgtgctg tggggcgcta acatggcgga gatgcacccg atcctctggt cacgcatcac
    14161 taaccgtcgt ctctctaacc agaacgtcac cgtggcggtg ctttctacct accagcatcg
    14221 tagcttcgag ctggcggata acggcatcat ctttacgccg caatctgacc tggtgatcct
    14281 gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact tcttcagcaa
    14341 gcacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc cgacccatcc
    14401 gctggaaaaa gcagcgaaga atccgggttc tgacgcctcc gaaccgatga gctttgaaga
    14461 ttacaaagcc ttcgttgctg agtatacgct ggaaaaaact gccgaaatga ccggcgtgcc
    14521 gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga aagtcatctc
    14581 ctactggacg atgggcttca accagcatac tcgtggcgtg tgggccaaca acctggtcta
    14641 caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt tctccctgac
    14701 cgggcagcct tccgcgtgtg gtactgcgcg tgaagtgggc acctttgctc accgtctgcc
    14761 tgcggacatg gtggtaacta acgagaaaca tcgtgatatc tgcgagaaga agtggaatat
    14821 cccgagcggc accattccgg cgaaaatcgg tctgcatgcg gtagcacaag accgtgcgct
    14881 gaaagacggc aagctgaatg tttactggac catgtgtacc aacaacatgc aggccgggcc
    14941 gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca tcatcgtctc
    15001 cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga ccgcaatgtg
    15061 ggtagagaaa gagggcgctt acggtaacgc cgaacgccgt actcagttct ggcgtcagca
    15121 ggtacaggcg ccaggcgaag cgaaatcgga tctctggcag ttagtccagt tctcccgccg
    15181 cttcaaaact gaagaagtat ggccggaaga gctgctggcg aagaaaccgg aactgcgtgg
    15241 caaaacgctg tacgaagttc tgtatgccac ccccgaagtg agcaaatacc ctgtatccga
    15301 actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc tgcaaaaagg
    15361 gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctgg caccgttcga
    15421 tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag aaacgcagtg
    15481 gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctaca agttctacgg
    15541 taaaccggat ggcaaagcgg tgatcttcgc gctgccgttc gaaccggcgg cggaagcacc
    15601 ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact ggcacaccgg
    15661 cagtatgact cgccgtgtac cagaactgca ccgcgccttc ccggaagcgg tcctgtttat
    15721 tcacccgctg gatgcgaaag cgcgcgatct gcgtcgtggc gacaaagtga aagtggtttc
    15781 tcgccgtggc gaagtgatct cgattgtcga aacgcgcggt cgtaaccgtc cgccacaggg
    15841 cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactga cgctggatgc
    15901 gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac tggagaaggt
    15961 gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct ttctgcgcga
    16021 tgtcgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt tacaacagca
    16081 aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg agaacgcctt
    16141 tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg acaccttaaa
    16201 actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg cacgggatat
    16261 tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccaagcg gtgcgctgga
    16321 tcgtgagatt gaatcgatcg acgacgcgcg gatggggctg gcggtactgg tggaccagga
    16381 aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat gcccgaaaat
    16441 tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc acgcccgctt
    16501 tctgccgacg gttcacagcg acgcctgtac cggttgcggt aagtgcgaaa aagtatgcgt
    16561 gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg agttaggtca
    16621 ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt gacgccgggc
    16681 gcgaggcgct ggagaaaaaa ggctggtggc gcagtcaccg ttggctggtg ttacgtcgcc
    16741 tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt gtgtggatct
    16801 tacacggtaa ctacagcagt agcctgttat tcgacaccgt tccgctgacc gatccgctga
    16861 tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg accggtgcgg
    16921 tgattatcac cgtgctctac gccctggcgg gaaagcgatt attttgcagc tgggtctgcc
    16981 cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac ctcaatcagt
    17041 ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg gtgggatcag
    17101 ccttaactgg cacgctcatt tgggaatgga tcaaccccgt ttctctgatg gggcgcagcc
    17161 tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta tttgatctac
    17221 tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg tatggcgtgc
    17281 tgggtagcaa aggtgtgatt accgttgcgg ccaccgatcg gcagaaatgt aaccgctgta
    17341 tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg ctggatgaac
    17401 aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc gtggatgtct
    17461 gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa tcatgaaaag
    17521 ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg tggtaagcgg
    17581 cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat ccgggacaca
    17641 ggaaggggcc attcggatgc caaaagagca ggaacggatg ccgctgaact atgtgaatca
    17701 gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata ccaaccgctg
    17761 cttgcagtgc cacggtgtcg aaagttatcg caccactggc gcgccgcgta tcagtcctac
    17821 tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc gttatttctg
    17881 tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata cctttacccc
    17941 atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta agcctggtct
    18001 gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg ggacgctgct
    18061 gttgatcggt tttgttggcg gcatcgtctt ctggggtggc tttaacaccg ggatggaaaa
    18121 agccaatacc gaagagttct gcattagctg ccacgaaatg cgcaacacgg tgtatcagga
    18181 atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct gtccggattg
    18241 tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa gtaaagagct
    18301 gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc atcgtctgac
    18361 gatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt gccgtaactg
    18421 tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga agatgcatga
    18481 ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag cgcacaagct
    18541 gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg gtatgcttga
    18601 agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct tgtcatttac
    18661 gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga agacaacgct
    18721 tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct ggcaagggca
    18781 gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag gccatcagcc
    18841 ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc gcgatggcga
    18901 taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg aagatattcc
    18961 tgtaaatcag ctctcggccg ggcaacaacg ccgcgtcgct ttagcgcgtc tgtggctgac
    19021 ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta acggcgtcga
    19081 tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga ttctgactac
    19141 ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga cgcaaacgag
    19201 ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt tcgccatagc
    19261 gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt tccgctcagt
    19321 atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg ggttgctgcg
    19381 ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca ggacggcagt
    19441 cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc gaaggtgatg
    19501 gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt agcaatgcta
    19561 ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgcttctgct gggaacgcct
    19621 acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa gcgcggtggt
    19681 gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt tgccaccgcc
    19741 gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat tttaggcgcg
    19801 ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt acgaatcagc
    19861 attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact atgtggaaaa
    19921 cactgcatca actggcgatc cctccacggc tgtatcaaat ctgtggctgg tttataccgt
    19981 ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga ttcggctttg
    20041 ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat gtgcctgcgg
    20101 cgatctggtc gatgggcatt tatgcatcaa tggcggtggc ggcttttatt ggccttgtct
    20161 ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt gccgtgttta
    20221 cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc acctggtggg
    20281 tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg ggtgtgattg
    20341 ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt atcctggtgc
    20401 tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg aacaccctgc
    20461 atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg ccgctgcgct
    20521 ggtcgatttt tggcttcctg ctgctgtctg ccacgctgac gctgatgcgg atgcgtaatt
    20581 tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg aaaagaggcc
    20641 gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg gttacgcctt
    20701 ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg tcgtgcactc
    20761 ggtgatgcag catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc gtgaggcgcg
    20821 tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa ccgcttgtgg
    20881 attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct atatgcgctg
    20941 cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa gcgtgaaact
    21001 cagcaaatgc cggaagtcgg gcagcgtctg cgcgtcggcg ggatggtgat gccgggtagc
    21061 gtgcagcgcg atcccaattc gctaaaagta actttcacca tttacgacgc tgaaggttca
    21121 gtagatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca gggcgttgtg
    21181 gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct ggcgaaacac
    21241 gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca ccgtcgcccg
    21301 gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg ctgctgtgcc
    21361 tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg gcgcgcggag
    21421 atgcgcgcat gatggcgtct tcccgcttgt ttgcctggct gctgtttatg tctgtggctg
    21481 gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc acctatgttg
    21541 ccagcaactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc tggggcgcgc
    21601 atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt gcggtggcga
    21661 tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata atggggatgg
    21721 tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca cgcacgctgc
    21781 cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggatccg gggctgatct
    21841 tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt gcttttgcca
    21901 ttgcttcttt gttgtgcggg cgtctggaca gcacttatgc gcgttttact cgtccgtgga
    21961 cgctggcggc gtggatcttc ttgacgctcg gcattgtgct cggttccgca tgggcctatt
    22021 acgaactcgg ctggggcggc tggtggttct gggatccggt agaaaacgcc tcgtttatgc
    22081 cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa cgcgccagct
    22141 tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg ctggggactt
    22201 tcctggtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcctctgat ccggcacgcg
    22261 gtatgtttat cctcgccttt atggtactgg tgattggtgg ttcgctgctg ctgtttgccg
    22321 cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg gaatctctgc
    22381 tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtattactg gggacgctgc
    22441 tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa ccgttcttca
    22501 acaccatgtt tacctggctg atggtgccgt ttgcgctgct gcttggtgtc ggtcctctgg
    22561 tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc gccttcatct
    22621 caacgctggt gctgtcgctg ctgctgccgt ggctgttcga aagcaaagtt gtggcgatga
    22681 cggtgctcgg cctggcaatg gcctgctgga ttgctgtgct ggcaattgcg gaagctgcgc
    22741 tgcgtatttc acgtggcacg aaaaccacct tcagttattg gggaatggtg gcggctcacc
    22801 tggggctggc agtgacaatt gttggtattg cctttagcca gaactatagc gttgagcgtg
    22861 atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc accttccgtg
    22921 atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc ggcgtaacgc
    22981 gcgatggcaa gccggaaacg gtgctgtatg cggaaaaacg ttattacaac actgccgggt
    23041 cgatgatgac cgaagcggca attgacggcg gcatcacgcg tgacctgtac gccgcgctcg
    23101 gtgaagagct ggaaaacggc gcgtgggccg tgcgtcttta ctacaaacca tttgttcgct
    23161 ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg tttgatcctc
    23221 gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggccgtatga agcgcaaagt
    23281 attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt ggcagctggc
    23341 gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg gcaagcctgt
    23401 gccgaagttt cgtctcgaat cactggacaa tccggggcag ttttatcagg cggatgtgct
    23461 gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga cctgccgtgc
    23521 ggaacatcaa tatctgaatc agctttcagc gcagggcatt cgcgtggtcg gcatgaacta
    23581 taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc cttacgcgct
    23641 aagcctgttt gatggcgacg gcatgttagg gctggatctc ggtgtctatg gcgcgccaga
    23701 aaccttcctt attgacggca acggtatcat tcgctatcgc catgcgggcg atctcaatcc
    23761 tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg aggccgcaca
    23821 atgaggtttt tattgggcgt gctgatgctg atgatctccg gctcagcact ggcgaccatc
    23881 gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct cactgaagaa
    23941 ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat gattgccacc
    24001 gacctgcgtc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa agagattgtc
    24061 gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt aacgccgctg
    24121 accgtgctgc tgtgggtgct tccggtagtg gctattggca ttggcggttg ggtcatatac
    24181 gcccgctcgc ggcgtcgggt acgcgtagtg ccggaagcgt ttcctgaaca aagcgtgcag
    24241 gaaggtaagc gtgccggata tattgtttat ctgccgggta ttgtggtggc gttaattgtg
    24301 gctggcgtca gctactacca gactggcaat tatcagcagg tgaaaatctg gcagcaggcc
    24361 acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagccga tccgctcaac
    24421 gaagaagaga tgtcgcgtct tgcgctgggg atgcgtactc aactgcaaaa aaatccggga
    24481 gatatagaag gctggattat gttgggccgc gttggcatgg cgctgggtaa cgccagtatc
    24541 gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga tgccgcactg
    24601 ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct cggcggcgaa
    24661 ctgctgcgcc agttggtgag aacggaccat agcaatatcc gtgtgctaag catgtatgcg
    24721 tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga gatgatgttg
    24781 aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat cgcgcaggcg
    24841 atgcaacatt tgtcgctgca ggagagtaaa taaaaagagg cccgatggtg atgccatcgg
    24901 gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc acgcgtgagc
    24961 ggacattgag tttacgcagc agattgcgaa tatgtacttt tactgtctgc tcggaaatat
    25021 tcaataccga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc agaacatcca
    25081 gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt tcacgttcac
    25141 gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg gcacgaatcg
    25201 cttccagcaa tacttccggg tcgctgtctt tcaacagata accgtctgcg cctgcgtcta
    25261 tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt tgcgcggtaa
    25321 cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc atattgagat
    25381 ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc gcgccgtcgc
    25441 ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga acaccgcgtc
    25501 gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca ggcatagtag
    25561 tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac gctaccaggc
    25621 gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac cttgtcgttc
    25681 gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca tcaagaaaaa
    25741 caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta aaaactgact
    25801 ccgggtatgg agctatgggt attttccgta cccaatgctt ttaacagcaa ttaatttcat
    25861 aggatgaaag ctcaattcat caatctggtt ctgtttctct ttgtcgttcc gcaatatctg
    25921 ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt gagtatggtg
    25981 agacagttga tggtgttgtc ctggaaaaag atatccagct ggtttatggg accgccaata
    26041 atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta agtagtaata
    26101 ctgaaattaa aggagggtat cagtacattg aaatgaatgg taccgcagaa tactcagtat
    26161 taaatgacgg ttatcaaatt gttcaaatgg gtggcgcggc aaaccagact accctcaata
    26221 atggtgtgct acaggtttat ggcgcagcga atgatcccac gattaaaggc gggcgcttaa
    26281 tcgttgaaaa agatgggatt accgtccttg ccgctatcga aaagggagga ttactggagg
    26341 ttaaagaggg gggattagcg attgcggtag atcagaaagc aggcggtgct attaaagcaa
    26401 gcacgcgggt catggaggca ttcggaacaa accgtctcgg tcagttcgaa atcaagaatg
    26461 gtattgctaa caatatgctg ttggaaaacg gcggaagttt gcgagttgaa gaaaatgact
    26521 tcgcttataa tactactgta gatagtggcg gcttactgga ggttatggat ggcgggactg
    26581 taactggcgt tgataaaaaa gcaggcggaa aattaattgt ctcaacgaat gcgctggaag
    26641 tgagtggtac aaacagtaaa ggccaattta gtataaaaga tggtgtgtca aaaaattatg
    26701 aactggatga tggttccggg ctcattgtta tggaggacac gcaggccatt gatactatcc
    26761 ttgatgagca tgccactatg caatcgctgg gaaaggatac tggtacgaaa gtgcaggcaa
    26821 atgcggtata tgatctcggt cgatcagatc agaatggaag tatcacgtat tcctcaaaag
    26881 ccatttctga aaatatggtt atcaacaatg gccgcgctaa cgtctgggct ggcacaatgg
    26941 ttaacgtttc agtcagaggg aatgatggca ttcttgaggt catgaagccg caaataaatt
    27001 atgcacccgc aatgttggtg ggtaaggtag tggtttctga gggcgcttct tttagaacgc
    27061 atggtgccgt ggataccagc aaagcggacg tttcgctcga aaatagcgta tggaccatca
    27121 ttgccgatat cactacgacg aaccaaaaca ccctcctcaa cttagccaac cttgcgatgt
    27181 ctgacgcaaa tgtgattatg atggatgagc cagtgactcg ttcatcagtg acggcaagtg
    27241 cggaaaattt cattacgttg accaccaata ccctgtcggg aaacggcaat ttttatatgc
    27301 gtaccgatat ggctaatcat cagagcgatc agctcaacgt caccggtcag gcaacaggtg
    27361 atttcaaaat attcgtgacg gacaccggtg ccagcccggc agcaggagat agccttacac
    27421 tggtaacaac gggcggcggt gatgctgcat ttacgttggg caatgccgga ggcgttgttg
    27481 atatcggtac gtatgaatat accttgctgg ataatggcaa ccatagctgg agtttggcag
    27541 agaatcgcgc gcaaattacc ccttcaacca ctgatgtgct gaatatggcg gcagcacaac
    27601 cgctggtatt tgatgcagaa ctggacaccg tgcgtgagcg tcttggtagc gtaaaaggcg
    27661 ttaattacga tacggcgatg tggagttcgg caattaacac ccgcaacaac gtgaccactg
    27721 atgcgggcgc tggctttgag caaacattga ccgggctgac gctcggtatc gatagccgtt
    27781 tctcccgtga agaaaccagc acaattcgcg gcttgttctt tggttactct cattctgata
    27841 ttggttttga tcgcggcggc aaaggcaatg tcgatagcta taccctgggg gcttatgccg
    27901 gttgggagca tcagaacggt gcctatgttg atggagtggt gaaagttgac cgttttgcca
    27961 acaccatcca ttgcaagatg agtaatgggg caacagcgtt tggcgattac aatagtaacg
    28021 gcgcgggtgc tcatgtcgag agcgggttcc gttgggttga cggattgtgg agtgttagac
    28081 cctatctggc ctttaccggc tttaccacag atggtcagga ctacacgtta tcaaacggca
    28141 tgcgcgcgga tgtgggaaat acccggatat tacgcgctga agcgggaacg gcggtaagct
    28201 atcacatgga cctgcaaaac ggtacgacgc tggaaccctg actgaaagcc gccgtgcgtc
    28261 aggaatacgc cgattctaac caggtgaaag ttaatgacga tggcaaattt aataatgatg
    28321 tggctggaac ccgtggcgtt tatcaggctg ggataaggtc atcgtttacc ccgacgttaa
    28381 gcggtcattt gtcagtcagc tatggcaatg gcgcaggggt agaatcgccg tggaatactc
    28441 aggcgggtgt ggtctggacg ttctgataac aaaaaataaa caggctgtga tgtgtcacgg
    28501 tctgtttatc gaattaattg cagatataaa aaaaaccaac cgtaaaggtt gattttttct
    28561 tgggattttt ggtcggcacg agaggatttg aacctccgac ccccgacacc ccatgacggt
    28621 gcgctaccag gctgcgctac gtgccgactc gtggctgcta atactaccgt tttccacacc
    28681 gattgcaagt aagatatttt gctaactgat ttataattaa tcagttagcg ataaaacgct
    28741 tctcgtctgt cagcacttgc aataacaaac ttaactgtgg tttttcatct ttcactcttt
    28801 caccacgtaa gttgtaagtg cggtatttac cgttattgtt cagcaccagc gtctttttcg
    28861 gggtggtaat tgccagcgta tcgttatccg ctgcagtaac ccagtaatga cggcgttgag
    28921 ggttgaacaa atcctgacct tgcgaatatt cgctggcagg tgtgctgaca tgtagcaggc
    28981 gttgcatcag cgtcgtcatc agatcggtat gatcagtcag agcattaata cgctgcgccg
    29041 gcgtgcctgg ccagtgaatc actaatggca cctgcagatg accgtgggac cagtcaaagg
    29101 tttcttcgtc ttcgctcagc ggaatacccc gaccggcagt gataatcacc accgtattgt
    29161 ccagtttgcc agaatcacgc agtgcattga gcacgcggtt gatctggtca tcgacattgc
    29221 ctgccgcccg gctatatttc cgtgcaaatg cctgctgatt gctgtcgtca atgttagtgc
    29281 cattgaaaga gacccacgag aaccagcggt tatcttcttg tgcgtagcgg cccagccagt
    29341 tgatccactg cgtggcggtc tgctcgtcgg attgggtgcg tacgctcggc atcgagaaat
    29401 ctgacaacaa tgcctggcga tacagcgggc tggtaaagcc atctgatgag aataatccca
    29461 gctgatagcc ttgctgatta agcgcagtaa ttaacgccgc aggggtacgg gtcgacagaa
    29521 tgccgtccat atagctcggc gagatgccat agaacaggcc aaagatgccg ttgtctgtag
    29581 tgttgccgga gctcatatgg cgcgtgaacg aaatattttg ctcagcaaaa cctgccagcg
    29641 caggcatctg cttctcgaag cgtgagtagt tcaggccatc gacagtaatc aacagcacat
    29701 tctgcccggt gcccatatcg cgatagcgca gttcgcttaa cggatactga acggaaacgg
    29761 cgtctggatt accttgctca ataagacggc gttgatactc ctgcgcatca agcagaccat
    29821 gcttctcaag aaaacgtcgc gccgtcatcg ggtacgaaag cggcaggtta gcgcgctgca
    29881 tggtgatcgg gcgatagaag ttggcatcgg cccagatata caccacatgc gaggcgataa
    29941 aggcgataaa taagaatgcg gccagcgggc gcgcgaagcg ccgacgacgc gtcaggctgc
    30001 gcagcttttg ccagctccac gtcgcaaaca ccagttcaag caataaaata accggcacgc
    30061 tgatgaacat cagctgccag tcgcgcgcca tctcattttc gtctgggttg ataaccagtt
    30121 gccagacgat gggattaaga tggagatgga aacgagtaaa gacttcgcta tcgatcagta
    30181 atagcgtcat tcccgccgtt gccagaatga cggacaaaaa cctcatcagc ctctgggagc
    30241 cgacgataaa ggtcagcggg aagaggatca gcaagtaggt ggcgaacacc aggaagctga
    30301 aatggccgat aatgcttacg taggaataaa tgcgaccagc aagcgttgtc ggccagtcgg
    30361 cgataaacag gtaacggctg ccaatgacga gcgaaagcag aatattgaac agtgcaaacc
    30421 agtgccccca actgaccatc tgggagactt tttcacggta gcgctgacga tgagttacca
    30481 taacctgtta tctgtttccc ttagtgcgct ttgtcttcgt tgatagagga ctgtaaggcg
    30541 cgggcaaaag agttggcaat tgcctggcgt tgggccgggg caatgctggt attgataagg
    30601 ttagtgacca tgttgcctaa caccatcagg gaaaggtcgg tcggagcctt atgtttttcc
    30661 agtacgttga gcagctctgc gagcaattgt tcaacctgtt catcactgta gcgggaaatt
    30721 tgtggcataa atcgaaatca gttagttgat gaaagggcaa catattaccg tagcaacggt
    30781 tttttttctg cttttttatg cgttaaatca ttctcgttac gctgccttct gtaaaatgac
    30841 cgccatcttt tattgcgtcg cttaaccggc ggtggttgaa taccgcccgg tcttaaagga
    30901 gagtttatca tgagtctgga tatcaaccag attgctctgc accagcttat caagcgtgat
    30961 gagcagaacc ttgagctggt gttgcgcgat tcattgctgg aaccgacaga aaccgtcgtt
    31021 gagatggtgg cagaactgca tcgggtctat agcgccaaaa ataaagctta cggcttgttt
    31081 agcgaagaga gtgaactggc gcagacgctg cgcttacagc gtcagggcga agaggacttt
    31141 ctggcattta gccgcgcggc aaccgggcgc ctgcgtgacg aattagcgaa atatcctttt
    31201 gctgacggtg gattcgtgct gttttgccac taccgttatc tggcggtgga gtatctgctg
    31261 gtggcagttc tgagcaacct gagcagtatg cgcgttaacg aaaatctcga catcaatcca
    31321 acccattatc ttgatatcaa tcatgcggat attgttgcgc gtatcgattt aaccgaatgg
    31381 gaaaccaatc cagagtccac ccgctatctc actttcctga aagggcgagt agggcgcaaa
    31441 gtggcggatt tctttatgga tttcctcggg gccagcgaag ggttaaacgc gaaagcgcaa
    31501 aaccgtgggc tgttgcaagc ggttgatgat ttcactgccg aagcacaact ggataaagca
    31561 gagcgacaga acgtgcgcca gcaggtttat agctactgta atgagcagct gcaggcaggc
    31621 gaagagatcg aactggagtc gctgtcgaaa gagctggcag gcgtcagcga agtgagtttc
    31681 acggagtttg cggctgaaaa aggctacgaa ctggaagaga gcttcccggc agaccgtagc
    31741 acactgcgtc agttgacgaa gtttgccggt agcggcggtg gcttgactat taactttgat
    31801 gcgatgctgt taggtgaacg catcttctgg gatccggcaa ccgataccct gaccattaaa
    31861 ggcacgccgc cgaatttgcg tgaccaattg cagcgccgga cgtctggcgg caattaatcg
    31921 gttttgtgtt ctgaatgcca gatgcggcgt gaacgcctta tctggcctgc aaaaaatagc
    31981 aataccgtaa tcaggcataa tctgcagata caaaaaaccc cgccggagcg gggtttttta
    32041 caacttattc agcaattaag cgcgaacgaa gtcgatgtgc tgcagcttcg gtttgtacgg
    32101 gtgacgctgt acgtcctgag ctttaacttt gatttcttta ccgtcaacaa cgatggtcag
    32161 aacttcgctg tagaattcag ctttagcttg catgttcatg actttgtcgt gatccagctc
    32221 gatagccagc ggcgcttctt tgccaccgta gatgattgcc gggaacttgt tagcggcacg
    32281 caggcggcgg ctcgcaccct taccctgctc tttacgtact tctgcgttga tagtaaacat
    32341 ttctttctct cttaaattaa tcctgctaca ggcgacccag caacaggtaa gtgatctgct
    32401 ttgcgtatgc aaaagcgggc gagattctat actcaatcgc tttatacatc aatcaaaact
    32461 accattaacc gcgtaattcg tgcgcccgac gaaaacgacc ttcgtaatcg aacacttttt
    32521 cacgcacttg ccagtactga cctttcatgc gggcaacgac aaaatccggg tgtcgcaata
    32581 aggcttgctg ggcgaggata tcggcggcgg tgatccagcg cagagggatg cccggtgtgc
    32641 gcgtatgcgg gcggataaaa agctgctcga aggcagttcg ctgggcgggc gtttgcagac
    32701 ggaaacgctc actcacatcc gcgccgtctt catcgtaata ggtgattttc aaccattcgc
    32761 ctttctcgtc gtgctcatgc tgcaaagaca tgccgctaca gcgtaatacc agcgcatctt
    32821 tcagtcgtag cgccgctttt aacatatcat ccggatcaac cagtaccgtg tcacattcgc
    32881 ggcagcggcg agcggcaata tcattttctg cgttacattg cgggcaattt ttaaaacgga
    32941 aacggaagtc acactgttcg cgatgaccgt cgtcatcttc aaaccatccc tgacagcggc
    33001 gaccaaagtg ttcaatcaat gtcccgtcgg cggtcgtttt cccccaaaag gtgttggcaa
    33061 aaccgcaggc agggcagaaa acctgtaccg gaacgttgtc acttttgcct ttcggtgtac
    33121 caacttccgg cgcgtagaga tcgtgaggat tacccgcata atcaagaatt aaacaatcag
    33181 tcttgcccgg agcgagacgc agaccgcgcc cgacaatttg ttggtaaaga ctcactgatt
    33241 cggtagggcg cagaatggcg ataagatcga cgtgcggggc gtcaaatccg gtggtcagta
    33301 ctgcgacgtt gaccagatag cgaaaacgct gggctttaaa atcttcaatt aacacatcgc
    33361 gctcagcgcc gggggtgtcg ccagtaatca gtgcagcatc ttcggcaggc agtaatccca
    33421 caatctcttt tgcgtgttca acggtggcgg cgaaaatcat tacccctttg cgcttttcgg
    33481 cgaactccat gatctggctg atgatatgtg gcgtaatgcg ttgctgtttt ttcagctcac
    33541 ggttgagatc agcttcgctg aacagcccgt tactctgtgc ctgcaagcgg ctgaaatcgt
    33601 attgcactac tggcatatcc agtcgttctg gcggcgtcag atagccgtgt ttaatcatat
    33661 aacgcagcgg cagctcataa atgcagtcac ggaaaagggc tttctcatcg ccgcgtacca
    33721 tgccgtgata atggaactgg tagatccagc ctttgcccaa tcgaaaaggc gtggcagtca
    33781 gccccagcag gcgtaagtgg ggattcactt tagtcaggtg agtgaggatt tgctgatact
    33841 ggctctcttc atcgtcacca atacggtggc attcatcgac aatcaacagc gaaaattccc
    33901 cctgaaaggc atcaagatta cgagcgaccg actgcacgct gccaaatacc actttaccgt
    33961 ggctctcttt gcgctttaac ccagcggcaa aaatatcggc ttccagcccc agcgcctgat
    34021 actttgcatg gttttgcgcc accagttctt tgacgtgtgc cagcaccaga acacgaccac
    34081 gcgccagccg tgccagttcg gcaatcacca ggcttttacc tgcgccggtg ggcagcacga
    34141 taacggcagg ggttttatga cgacgaaaat ggttgagcgt ggcatccacg gcttcttgct
    34201 gatatgggcg aagtgtaaaa atcatggtct cgcgtaggta gattattctg ctaatagtat
    34261 gccatggatc tttttccctt gagggatgtt attagaccgc tatactgggc agacaacagt
    34321 tcagattttc gggtcaatgc ccgacctccg tttttacagg caaaatcaca cacatgcgac
    34381 ttgataaatt tatcgcacag caactcggcg ttagccgtgc tattgccggg cgtgaaatcc
    34441 gcggcaatcg tgtcaccgtc gatggcgaaa tcgtccgtaa tgcagcgttc aaactgcttc
    34501 ctgaacatga tgtcgcttac gatggcaacc cgctggcgca gcaacacggt ccacgttact
    34561 tcatgctcaa taagcctcag ggctatgttt gctccacgga cgaccctgat cacccaacgg
    34621 tgctctattt tcttgatgaa ccggtagcgt ggaaactgca tgcggcgggg cggttggata
    34681 ttgataccac cggtctggtg ctgatgactg atgatggtca gtggtcgcac cgcattactt
    34741 ctccgcgcca tcattgcgag aagacctatc tggtgacact ggaatcacct gtagctgacg
    34801 atacggcaga gcaatttgct aaaggcgtgc agctgcataa cgaaaaagat ctcactaagc
    34861 ctgcggtgct ggaagtgatt accccaacgc aggttcgtct gaccatcagc gaagggcgtt
    34921 atcatcaggt gaaacgcatg ttcgccgccg tgggtaacca cgtggttgag ctgcatcgtg
    34981 aacgtattgg cggtattacg ctggatgctg atttagcccc cggtgaatat cgtccgttaa
    35041 ctgaagaaga aattgccagc gtcgtctaac ttttcaatga ctttcaggag cccgttgtga
    35101 ccacccgaca gcattcgtcg tttgctattg tttttatcct tggcctgctg gccatgttga
    35161 tgccgctgtc gattgatatg tatctgcccg cgctaccggt aatttcagcg cagtttggcg
    35221 taccggcggg cagtacgcag atgaccctca gtacttatat tctgggcttt gcgttggggc
    35281 agttaatcta cgggccgatg gcagacagct tcgggcgtaa gccggtggtg ctcggcggta
    35341 cgctggtgtt tgccgccgcc gcggtggcgt gtgcgttggc aaacaccatc gatcagctga
    35401 ttgtgatgcg tttcttccac gggctggctg cggctgcggc cagcgtggtc attaacgccc
    35461 tgatgcgcga tatttacccg aaagaagagt tctcgcggat gatgtcgttt gtcatgctgg
    35521 tgaccactat tgcaccgctg atggcaccga ttgtcggcgg ttgggtgctg gtgtggctga
    35581 gctggcatta catcttctgg atcctggcat tagcggcgat tctggcttcg gcaatgattt
    35641 tcttcctgat taaagaaacc ttaccaccgg agcgtcgtca gccatttcac attcgtacca
    35701 ctattggtaa ctttgcggcg ctgttccgtc ataagcgtgt cctgagttat atgctggcca
    35761 gcggtttcag ctttgccgga atgttctcgt tcttaagcgc cggaccgttt gtctacattg
    35821 aaattaacca cgtcgcgccg gaaaactttg gttattactt tgcactaaac attgttttcc
    35881 tgttcgtgat gaccattttt aacagccgct tcgttcgccg cattggcgcg ttaaatatgt
    35941 tccgctcggg gttgtggata caatttatta tggcagcgtg gatggtcatc agtgcgctgc
    36001 tggggctggg attctggtcg ctggtggttg gcgttgcggc gtttgtgggc tgcgtgtcga
    36061 tggtgtcatc caatgcgatg gcggtcattc ttgatgagtt tccccatatg gcgggaacgg
    36121 catcttcgct ggcaggaacc ttccgttttg gcataggggc aattgttggc gcattgcttt
    36181 ctcttgcgac ctttaactct gcatggccga tgatttggtc aattgcattc tgcgcaagca
    36241 gctccattct cttctgtctg tacgccagtc gaccgaaaaa acggtgatct attgcacaac
    36301 gaggaagcta aaaggcttcc tttgttgatg catgtcaacc acaaatctat cattcccccg
    36361 atatatgttt attttatgta aaatcaattt atgtaaaaag tcacatcatt gtagttaaaa
    36421 aggttgagtt agatcgcaga aacgggtaca tatagccccg caaacgtgac cacgcccgca
    36481 gatattactt aaatcagagc catagaggcc acgcaggcga ggcatcaatc tttacgatct
    36541 gtataaagac ggattgttga tgatgtgtta aaattgatgt aaacaaattg tgaagtgaat
    36601 gtacttccgg ggaaaataag tgacttcatt acaactctca atcgtccatc gactgccgca
    36661 gaactatcgc tggtctgctg gtttcgcagg ttcgaaggtt gaaccgattc cgcaaaatgg
    36721 accgtgcggt gacaacagcc tggtggcgct taaattgctt agcccggatg gtgataatgc
    36781 atggtcggtg atgtataaac taagccaggc gttaagcgac atcgaagttc catgttcggt
    36841 gctggagtgt gaaggtgagc cgtgcctgtt tgtaaatcgc caggacgagt ttgctgcaac
    36901 atgccgattg aaaaattttg gtgtggcaat tgccgaaccc ttttcaaact acaatccttt
    36961 ttaagcgtca gctcaacgcc agtagctgac gcgtatactc ctgctgcggt gtggcaaaca
    37021 cgcgcgcgca cggtccttgt tccactactt ccccttgtcg cagtacgata acctgatgac
    37081 ataacgcgcg gacaacgtgc aaatcgtggc tgataaacaa ataggccagt tgatgctttt
    37141 gttgcaatga tttcaatagc gtcaatattt gcgcctgtac cgttttgtcg agtgatgatg
    37201 tcggttcatc aagtatgatc agcgagggct taagaattaa tgccctggca atcgcaatac
    37261 gttgtcgctg accaccagag aactccgccg gataacggtg gcgtgtttca ggatctaatc
    37321 ccacttcatg catcacggct atcacttgtt gttcgcgttg tgcggcagaa agcgtcggct
    37381 ggtgaacccg taagccttcc tcaataatct gcaaaacgtt gagtcgtggg ttgagcgagg
    37441 agtttggatc ctgaaatacc acctgaatgc gatgacgaat aggtaacagc tggcggcgat
    37501 ttaaattttg cagtggctga ccgtcaaaga tgatgctgcc ctgagaatta atcagtcgca
    37561 gcagcgccag tcccgtcgta cttttcccgg aaccggactc gcccactaaa cccagtgttt
    37621 cacccgctcg tagcgtaaaa ctgatgtttt tcaccaccac attatgatcc acaatgcgct
    37681 tcaaaatccc tttgcgaatg gggaaggcaa cctgaagctg ttcaacatcc agcaacgttg
    37741 aggcaggttc tggcaacggc actggatcgc ctgacggttc actgttgagt agcttttgtg
    37801 tgtaaggatg agtgggtgag gcaaatagcg tagcggcgta attttgctcg acacagcgac
    37861 cgttttgcat taccgccacg cggtgggcca gttttctgac aatgctgagg ttatgagtaa
    37921 taaacagcat gcccatattc agctcgcctt gcagttcgcg caacagctgt aaaatctgcg
    37981 cctggacaga gacgtccagt gcggtggtcg gttcatcggc aattaataat tccggtcgcg
    38041 ttaacagcgc catcgcaatc atcacccgct ggcgttcccc gccggagagc tgatgcggat
    38101 aatctgtcag ccgttttgcc gcctggcgga taccaacgcg atcaaggcag ttaagaattt
    38161 cgccacgagc cgcttcccga cgcatcccgc ggtggagtga aagcacttca taaagctgtt
    38221 tttccagggt atgcaatgga tttaacgaca ccatcggttc ctgaaaaatc atggcgatct
    38281 tattaccgcg tacaccgcgc aacgtttgat cgctggcgtg aagcagcgat tcgccatgaa
    38341 aacgaatatc gccggagaga tattcaaccg gcggggaagg gagcaggcgt aaaattgaca
    38401 gcgcggtaac gcttttgcct gaacctgact cacccaccag cgccagcgtt tcgccagcct
    38461 caatctgtag tgaaacatca ttgactactg tacgtacggt ttgctgatgg cgaaaaccca
    38521 ccgacaaatt ttcaatcgct aacagagttt gcgtcatgct acaccgcctt attaggatca
    38581 aatgcgtcgc ggacggcttc accaataaag atcagcaaag acaataatat cgccaccgac
    38641 aagaaggcgg tgatcccaag ccacggggcc tgaaggttat ttttcccttg taacagcagt
    38701 tcaccgagtg acggtgaacc gagcggtaga ccgaagccga ggaaatcgag cgaggtcagg
    38761 gtggttatcg aactacataa aataaacggt aaaaaggtga gggtcgcgac catggcatta
    38821 ggcaacatat gacgcaggat gatactgcga tcgctgacgc caagcgcctg tgccgcacga
    38881 atgtagtcga aattacgagt acgtaaaaac tccgcccgca ccacgccgac cagactcatc
    38941 cagccaaaca agacagtaat tgccagcagc caccagaagt taggctgtac gacgctggaa
    39001 agtaaaataa tcaaaaacag cgtcggcatc cccgaccata cttcaataaa gcgttgtccc
    39061 cagagatcga ctttaccgcc gtaatagcct tgtagcgccc ccgccagcac gcccatcacg
    39121 ctggaacaga gagtcagcat caggccaaac agaaccgaga tccgcgtgcc atagagaatg
    39181 cgtgccagca catcgccgcc gttggcatcc gttcccagcc agttttgccg ggagggtgga
    39241 gaagggaagg gcttatcggt agcaaagttg atactggtag caccaaagcg aatcggtgcc
    39301 cacagtaccc agccgttatt ttccagccgt tgtttcagcc acgggtcctg ataatcagcc
    39361 tgacttgcca gcgggccgcc aaaatcgctt tcgctgtagt ttttcaacaa cgggaaatac
    39421 caactgccgt cataacgtac cagcaacggt ttatcgttgg cgataagttc agaacacaaa
    39481 ctcaaaccaa acaaaacgag gaaaatccat aacgaccagt agccgcgacg gttatggcga
    39541 aaacgcgccc aacgggcctg attgacgggg ctgagtcgcg acattaacgt ccctcaaaat
    39601 ctatacgcgg atcgaccagc gtatagctga tatcactgac aatattcagc agcaggccaa
    39661 tcagggtgaa aatatacaag gtaccaaaca ttacaggata atcgcgcgag acggtcgctt
    39721 cgtagcccag taagccaagg ccattgagtg aaaacatcac ttcaatcagc agcgagccgg
    39781 taaaaaacat gctgataaac gtcgccggaa aaccggcaat caccagcagc atggcgttgc
    39841 ggaacacatg tttccagaga atattttttt cacttacccc tttcgcacgc gcggtcacca
    39901 cgtattgctt gcgcacttca tcaaggaatg agtttttggt cagcatggtc agcgccgcaa
    39961 agccaccaat cactgtcgcc agcaccggca gcgtgatatg ccacagataa tcggtgattt
    40021 tctgatacca cggcagcgaa tcaaagttag cggaaaccag gccgcgtaga gggaacaggt
    40081 cgaaatagct gccgcccgcg aagaagacaa tcagcaggat ggcaaacaaa aaggccggaa
    40141 tggcgtagcc gatgatgata aatgcgctac tccagacgtc aaagcggctc ccattataaa
    40201 cagctttgcg aatgcctaac ggaatcgaca ccagatagat aatcagcgtg ctccacaatc
    40261 cgagggtgat ggaaaccggc agactgtctt taatcagcgt cagcaccgag gcgctgcgaa
    40321 aaaggctatc accaaaatca aagcggatgt agtcccagag cattttgaag taacgttcgt
    40381 ggatcggctt atcgaaaccg tagcgatgag tgatctcggc gatcacttct ggatctaatc
    40441 cgcgtccgcc acggtaatta ctgtcgctga tattgccgac gcccgtttgc gcatggctgg
    40501 cgcgaatacc ttcaccgcct gcgccgggta atactccggc attaccaaac tcaatggcgg
    40561 cgatggcctg gtcgaccgga ccgccaggcg caatttgcac gatgaaaaag ttgatagtga
    40621 taatcgccca taacgttggg atcaccagca acagacggcg aatcagataa gcgcccatct
    40681 actctccctg ttgtctggct gacggcagtt tggccgcttt attaacgtca taccaccagg
    40741 tatcgatacc caggctgtaa acagggcgta cagcgggttg ggagaattta tcccaccagg
    40801 cgagacggtc ttccgccatg taccacattg gcagcatgta ataattccac gttaatacgc
    40861 gatccagtgc tcgccctaac ggcagtaatt tttctttatt tccctgcgcg gcaataattt
    40921 gattgatcag cgagtcgata accggacttt gcacgccggg ggcattataa gtggaattga
    40981 tatattccga tgaccaggaa atctgtaaat cggaactggg ccacggcatc gcccgccata
    41041 cgcgcggcat catgtcatag tcgcgactgc gcatgcggtt ggtgatttgc gagttatcca
    41101 ccttgcgaat gtccatgttg atacccagcc gttgcaggct gtgctggaac ggcaataccc
    41161 actgactatt gctgcttgcg ggaagcaata attcaaagct gagtggctga cccgttgtgg
    41221 cattaacgcg ttgctgaccc ttcagcaccc agcccgcttc gttgagaagt ttgtcggctt
    41281 ttaacaggtt gtcacgatcg tagccatcgc ctttagatac cggcggctgg tagatttgtg
    41341 tgaagacttc tggcggtaga tcttttttca ttggtgccag aagcaccagc tccgcggcgt
    41401 cggggtaatt tctggccgcg tattcggtat tctgaaaata actgttcgtg cgactccagg
    41461 cgttgtaaaa caacgccttg ttcatccatt caaagtcaaa ggcgagggtg attgcttccc
    41521 ggacccggcg atcgctgaat accggacgtt ggatattaaa cgccagccag cgcgtatcct
    41581 gggctgattc gttcttttgc tcatctttga tgatgtattt tttatcgaaa tttttaccgg
    41641 tataacgcgt ggcccagttt ttagcgtcgt tttccatacg caaatcaaag gcacctgctt
    41701 taaacgcttc aaaggcgaca ttatcatcga ggtaataatc gtagcgaatg gtgtcgaaat
    41761 tccagcgtcc acggtttacc ggtaagtttg ctgcccagta atctttcacg cgggaataga
    41821 caatattttg ccccattttc caggacgtaa cgcggtacgg accactggca agcggaggcg
    41881 tggcgagcgg gtcgctaagt ttgtgatcct tccagtactt ttctggaaat accggcagcg
    41941 aaaacagact cagcatatct tctttgctgg ctttagctaa ctcaatgcgc acggttaacg
    42001 gtgcaatggc tttgacggtg gtgcctttgt agaccagacg aaattgcggt acgccttcgg
    42061 tcataaattt tcgaaaagta aactctacat cgcgggcagt aatgggcgaa ccatcatgaa
    42121 aacgggcgcg cggattaata gcgacctcca cccaggaata atcgtcagca tagcgtgcgc
    42181 tttcagcaat cagcgggtaa taactgcctg gttcgtcatc ggaagtcgta aatagcgtgt
    42241 cgtacagctg ctcggtgcgt gcgcccgggt tgccgcgcag tgcatagcgg ttgaaattat
    42301 cgaaggtgcc gagggctgac aacgttatct gcccaccttt tggcgcggcg gggttcacat
    42361 aatcaaaatg gttgaaatta aacgcgtacc ggggttcgcc cagcacggca aaggcatagc
    42421 tttccttgat agcctgcgcc tgcgcaccaa aggtgaacag agcgataaac agcagcagta
    42481 tgcgcacaat catctgcaac ggatttcctt ttactcatca ttagccgcaa tccggcatgc
    42541 tttcactatg aataataagg gacgtaagca caccttacca ctgcggcgta tacggtttct
    42601 tcagccagcg aacaaaatcg tccagcggta acgggcgact aatccagtag ccttgcatga
    42661 aattaacgcc gcgttcgctt agccatcgcg cttgttccgg cgtttcgacc ccttcagcaa
    42721 ccgtcagcat attgaggcgt ttcgccagcg tcagcaccgc gtcaagtacg ggtgaagtga
    42781 tcgtttccgt accgatggcg ttgataaatc cacggtcaat tttcagataa tcgagcgtaa
    42841 aacgctcaag atagataagc gcgctgtgcc cggtgccgaa gtcatcaata gcaatttcta
    42901 cgcctaccga gtgcagccag gcgaagagtt gtgtggcttc ttgctctttc agcatatcgc
    42961 gctcggtaat ttccagcaca atctggaaat ggtgtgcggg tagggaagtg agcagtttct
    43021 ggatatctgc tttaaagctt tcgctgtgca gatggtccgg cgcaatgtta atgccaaatt
    43081 tgacgcctac cggcagcact ttttctaatt ctgcggcatc gcgggcaatt aactcaaaca
    43141 gatgctgagt cagcggcaca atcatctttt gcgattcggc aaagttaatg aaggcatccg
    43201 ggggaatttc tcccgcgaca ggatgccgcc agcgtagcag tacttccagg cccgttactc
    43261 gcaaagcttg tgtatccacc accggttgat acgccacgta aaattgttcg cgcttgatgg
    43321 cggtcatgat ttctctgccg gggcgcatac gtacgctcat caggtaatag cagagcaggc
    43381 caacgacagt tccactcatg ccgcccagta aaaatgcgta ccacacatcg ttccatgtcc
    43441 agtcatctgc ataaagccga acggtcagag gaatgcccgc aattttagtt tcacggactg
    43501 gcatgtcggt taattcgtta acgttcatca aacgtgaaga aaaggtagat agcgcagtat
    43561 tgccaataat gagggcgacg ccatcgtaat cttcctgccg tgaactataa aagagtgaag
    43621 gcgtcaggtt gagattcaga gcggcaaaga cgccgctatt tttcagcaaa gggttgcgat
    43681 accagatgac gattgcgggt ttgttcggca ccatcggcgt gccgggtaag atcgccatat
    43741 cgacattttt attaatgtcg agcgccggaa tcaattcatt gagtggaatg tccatctcac
    43801 cggtcgcaga tgaacagaat gtttttttat ctttcaccag cacaaacgta cggacattca
    43861 tgctaaacgc cgcgcgggcg gtcagttcgg ggttagcctg ctggcaggta tctaaggtca
    43921 gcggctggag ccggtcagta gtggatttca ggtcggcaaa ataggtatcg agatactttt
    43981 gtacgtcggt aatcagtgtg tcgtatttga cttcgtgctt atgccaggcc actaaaaact
    44041 gaaggcaact caccagtatc gcaatcatca cgcctgcaac aatgcaggta agcaggagct
    44101 tacgtccaga attgggagcg cgtatgaaca tgattccgtt aatttcctga aattacgaca
    44161 acatttaaaa gataacaatg accggactat agcctggatc aagacgcgga aacatcattt
    44221 tctctgatga atgaaaacaa aaaagcactg cctaagcagt gctttcagtt aactcgttag
    44281 gatagccaag ggattgcatc caaacggttt attagctgcg gctgagaacc cggcgtgctt
    44341 cgttgtaacg cttcttccag tacggttcat tcatgctgga aataataaca ccactgctgg
    44401 tggaagcatg gacaaactga ttgttgccga tataaatacc gacatggcgt cccgttgaac
    44461 cggcacggaa cagaactaaa tcacccgtac gcaaattact gcgggaaaca gatttaccca
    44521 tttcctgctg ttcgtaagtc gaacgtggaa gttctaagcc aaattgctca cggaatgtac
    44581 gctgtacgaa accagaacaa tcgatacctt ttttagtgct gccgcccaga cgataacgta
    44641 cgcctttcca gtcagcatac tgatccataa ttcgcgattt tacgtcgaca ttacgaacca
    44701 ggttttcaaa ttcatcctga gaagcttgca gtgatgatgt ttcactaccc actacacgtg
    44761 tctcaggatg catattcttt gcggtgttat ttgcactaca tgcagaaagc agaaccgcta
    44821 ctgcaatcgc ggggatcccg cgcaagatat atctcaaaat cggttgagat ttgaccatgt
    44881 tgtttgtttt cccttgaagt ccttaacgac aaatatcgtt ataaaaaaat gccaagcgac
    44941 acgagactaa ttacctgggc atgttgaaac aattccttaa ggccaaatct gtggactaac
    45001 gcacaaaatt atatgctgtg aggataatct accgccgagg caaaacgagt tcgagattac
    45061 ccgattggtg cggtcttggc gagcggtttt acatgatttt ttataagaaa aagtgataca
    45121 gaaagttaat aagcgggatt ggtcaattaa agtacgattg agtgaaacgc atgcaatcca
    45181 ttcatttact tagagaatga tttttgcgcg cgatggatga aagcacggtg cgcatcatca
    45241 ggattatcct cactataaaa ataaacctga tgatgttaat tactgtgagt tatttgtttt
    45301 ggaaatgttt gttttttccc ggtagtgatt tgtcaaaaaa ggtgattaat cgatcactta
    45361 atggcgtcag caacacccag ggcaaaccga tcaatatcac ggtcatcgaa ccgacaatga
    45421 tgtcagtaaa ccagtgtgcg ccaatcatta ctctgggaaa cgcaaaaacc acaaaaataa
    45481 taagggcgat aagacctgca actttgccga aataacgcca catgaatgcc gaaaaaataa
    45541 gcagcatcat gccgtgatcg ccgggaaagc tatctcgtga ggcatctttc gtgggaacag
    45601 agagcagttc gctgacgcgg ttaatgtcgg taaaagtcaa tgttgggctg gcccgtttta
    45661 caggaattaa tgcctgaccc agctggttta ataccactgc agttaatagc atgaccagac
    45721 caataatcac gatacgtcgt ctgcctgggg cgttttcttt cagccagaaa ctcagcatca
    45781 acatacccat cgccagcagt gaacaaccgt cgaaggcgcg attgttggtc aatgcaacca
    45841 gccacaaaaa ggccttactt tcgaccagtt tctgattaaa gaaataaaaa atatccgcat
    45901 caatcggcaa ccagaatcca tgattaacgg gaatatacca ggaaagaaac agcgcgaggc
    45961 cgacaatatt caacaacact atttgcggca aatttttaat catattttct ctaattatct
    46021 tactgaaaaa cgcaggcaac cttacgcggt agtcgctaaa cgaagcttca acagcgcgct
    46081 ctgcaaagca ttccagtcag cttcgctgct ggaaatcagc tcaatacgac tgtccggtgg
    46141 cgcaacgttt tgcgtttcaa tgtgcaggtc atcgccctga cggttgattc gtaccagccc
    46201 ttcgggaata cgcagcacgc ctttgacgcg ttccaccggt gcaagtcgcg cccattccag
    46261 aatgccaatg gtgtcgaata ccgtatcagc gtcgaatatc cagccgcagg cctgatatcc
    46321 ttgcccgctg ttcagactgc gacgccagcg ttgatgctct ggcaggctta acgctgctaa
    46381 cccttttttc acgacatgct gatgagaatg cgcggcgctg gcgggcaact cggctaaatt
    46441 gcgacgcggc aaatccagaa gatgaccgtc aactttccca tgctcactgt gaattaattg
    46501 tcgatcgcca ccattttgct gccaccaacg ctgtagcgct tgctcacttt cgggcgtcgc
    46561 acggtcggat ttattggcga caatgatgtc tgcggcagcc agctggtcac ggaagttttc
    46621 attgctggcg cttttttcgt ccagcagcaa gcgcggatcg agaatgcaca aggtggcgcg
    46681 cagatctatc cacggttcat agactggtgc ggttaacaga tcgaggatct gtttcggatg
    46741 gcccagcccg gtcggctcta tcaacaagcg gtctggtttt ccctgacgca gtaaggtatt
    46801 caaccctacc tgcatgggta aaccattaac gcagcacatg cagccgccgg ggatctcttt
    46861 cagcaatgcg ccgctatcgg cgagcaaagc accatcaatt ccgacttccc caaattcatt
    46921 aaccagtacc gcccattttt cgttgggatc tttatgggct aacagatgaa gaatcgacgt
    46981 ggttttcccg ctgccgagaa aaccggtgat gaggttggtc ctggtcacaa cggctccgca
    47041 atactctggt aagttaactg agcgttaatc ctggctaaaa aaggcagaaa agagaagggg
    47101 agagcggacg aaggtgagct tcatccgctt taaccgcaga aatcgttaaa ttttgagggt
    47161 gtttaatagc gcctgatgtg cgccgaattg tacgatttgt tgccaggctt gttcgatggc
    47221 ctgcacaaaa tgcgggttat ctggcagatc atcaccgaaa acttcacgca gggaaagcag
    47281 ggcggttacg cgttgttcac tgctgctgcc cgcaacaagt tcgcgaattt tatcgctaag
    47341 cggatcgcga acatcaatgg catttccggc atcatcaacg ccgctgacgt aacgcatcca
    47401 gccagcaacg cctaatgcca gcaacgacca gtccgtttcg caccccagat gtatgcgaat
    47461 acctgccagc atacgttgcg gtaatttctg gctaccatcc attgcgattt gccaggtctt
    47521 atgtttcagc gccggattag caaaacgtgc aattaacttg tccgcatatt gtgttaaatc
    47581 gacatcttta atttgtagtg tcggcgcttg ctcattcagc attaatgttc tggcggcatg
    47641 gcgaaatgcg cgatcctgca tgcaatcact gatatgggcg aatcctgaaa ggtaacccag
    47701 ataagcgaga aaagagtggc tgccattaag catacgcagt ttcatctctt cccatggcag
    47761 gacatcattc accatttgta cacctgcgac ttcccaggca ggacgcccag cgacgaagtt
    47821 atcttccact acccactgga taaacggttc gcagctaatc gcgcagggat catttacccc
    47881 cagatgctgg ctgatttctg ccagtgattc gtcggttgca gccggaacaa tgcggtcgac
    47941 catggttccc ggaaaactga cgtgttcttt tatccacccg gcgagttctg gcgaacgttt
    48001 ttctgccatt cccagcaccg cgtttttcac cacatggcca ttgtcgggaa tattgtcgca
    48061 ggagagcacg gtaaacggtg taaggccgcg ctcacggcgg cgtttcagtg cttcgacgag
    48121 aatacccggt gcggagtgag gttcttcagg ggtttgtaga tcgtgaataa tccgcggatt
    48181 actggtgtcg agtgcaccgg tggccgggtc aatacaatag cctttttcgg taatcgtcag
    48241 ggaaacaatt gccacctgtg gctcgcaaaa tttctcaata attgccgcta aggaatccag
    48301 ttttgcatta aggcattcgt gaacggcacc gacaattatc acctgattgc cgtccgcacc
    48361 tttttccagc acggtatata aatggttctg tgcgcggagc tggctcatca gttgatcacc
    48421 gctgaacaag ctgatttcac agatccccca gtcgccgccc tgggcattca gcacacgatc
    48481 ggtcagtaac gcctgatgag cgcggtgaaa ggcgccaaag ccaaaatgaa cgatacgtga
    48541 ttgcaactgc tggcgatcgt agcgtggagc gtgtacatga tgcgggagcg taacggaggc
    48601 aattgtcttc ataggagtca cgtcttagta acgataaatt aacaatgaca gtgtaaagtt
    48661 atatgacagc aattgaaatt ggtctggatt gttaatgcga tgaagtgtga gggcgatcaa
    48721 tcaacgagtg aaaattgagg gcgtaaggtg ttcaggaagc gcaaaataga agattggtat
    48781 aataaattaa aaaagggaca gcctgagcta tcccttttct gcatgcggct gaagttagtc
    48841 agcacgcccc atataacggc gttcttcgat atggatacga attttttcgc ccgggcttaa
    48901 gtattctggt acctgaatca ccagaccagt gctcaatgtc gccggtttgt tacgggcgct
    48961 ggcagatgcc cctttgatgc ctggtgcggt ttcaacgatt tccagatcaa cggtctgcgg
    49021 cagctcaagc gccagcagtt gaccgtccca ggtcagcacc tgcatgtccg gcatgccgcc
    49081 ctctggcata aacagcaact cttcttcaat ctgatctttg gtgaaggtat acggggtata
    49141 gtcttcttta tccataaaga catattcgtt gccatcgaca taggagaagt caacgtaacg
    49201 gcgggtcagc gtcacggtgt caacgatatc atcacctttg aaacgctctt ctactttcag
    49261 cccggtacgg acatcagaaa aacgcatttt gtacagcgta gcggcaccgc gggcagtggg
    49321 cgactgaata tcaatatcct taaccagcag cagtttgccg ttgtaattca gtaccatacc
    49381 ttttttaatt tcgttcgctc ttggcatcga aaaattcctg tagtcgaaaa tgtcaaaaat
    49441 atcgcgacaa agtactcgcg acgggccttt cgggcaagtg gtattcgcac ttttgctggt
    49501 gcaaaaaagg tggtactgtg cgcgctcatc aatccggtgg ttaaccttaa gagaacaacg
    49561 tatgaaatgc cgtccgggtt gcggggcgtg ttgcactgcc ccttcaattt ccagccctat
    49621 tcccggtatg ccagatggca agcccgccaa tacgccctgc attcagcttg atgaacagca
    49681 acgctgtaaa attttcacct cgccactgcg accgaaagtc tgtgcaggct tacaagccag
    49741 cgctgaaatg tgcggtaact cgcgtcagca ggccatgacc tggcttatcg atctggaaat
    49801 gctgaccgcg ccttaaacat ctttaatccg cagtaagcaa aacagagtgg cgataatcat
    49861 caccatcgca aaccagaaca cagcgtgata attccagatc tcggcgacga tgcccgccac
    49921 tgatcctgcg atgatccagc ccacgcgcga cgtgttggta tagagcgtgg tggctgaacc
    49981 cgcctgaccg ggcatcagat cctgaaaata gagcatcccg atgccgccca gaatgccaat
    50041 aaaaatagca tttagcagct gtaagcccaa cagaatgaca ggtgaatgcg ccatcagcat
    50101 tcctgcgtaa aaacagacgc cacccacggc agcaacgcgc attaagaaac gcttacccag
    50161 acgtttggcg aaatatccgg caatcaacat cgtcgggatt tccagcccgg cggcggtccc
    50221 catcatcaca ccggccagtt tctcgggaag atgcagttcg ttgataataa atagcggcat
    50281 gttgatgatg tacaggctgt tcgagcccca catcaatgta caaatgacaa acagcagcag
    50341 cgtatcgcga cggttacggc gcggcgcttc gatcgtgccg gtcgccagcg gaagctcttt
    50401 ttgcatcgac ggtaaaaaca gccacaccat cacaccgcaa acaataaacg ctaccgctgc
    50461 gctcagatac attaccgtaa agctgaaacc catcgctaag gcataagcca gcggtgggcc
    50521 aatgacccat gccagtgaaa cctgagcgcg taaaaaagag ctgaacatca ccgcctcacg
    50581 tccggttttg tcggcatgtt cacgggcaag ggcaaacatt tgcgggttag cggtcgagcc
    50641 aaagctgcta agaaagacgc caacgaatag caaaacaaag tagttgcgat tccaggcaaa
    50701 aagggtgcag gccagcacgc ctaacaggca gcaaaagaca atcagcgatt tgcgatcgcc
    50761 gcgcttatca gagcgcccgg cgagaaactg acttaccaga atcccaatga cagcgctgcc
    50821 ggtaaagaag aatcccacca tcgccggacg agcatgtact tcatcagtaa gaaaaatact
    50881 gagtgtcggg gtttgcagag cgcccgcaat accggtgaga aaggcaacga ttaaaaacgc
    50941 cgtcgaggtc aggtcaaacg atttcgcgct ggagactgcg ggggagttat gcatgctggt
    51001 cgatcactgg aagaggaaga cgcggagttt acgctgcttg tcagaaaata aacagacagc
    51061 gaaatcaaaa gcgccacgaa taatcaaaat gccatttcac tgttgctgat tggctgctga
    51121 cgctgctgaa atgaaattgc tgataacgga ttttcccatc agcaattagg aaaaatggca
    51181 aaaaattgtg cagcacatca aacttttgct cataacttta cggctttcct tgcgtgctga
    51241 aacagaagta ttatgctttc ttgaaacgtt tcagcgcgat cttgtcttta accctaagcc
    51301 aggttggcgc tttttttctc atagaggctg aatcgtttca attcagcaag agaggagaac
    51361 tatgttccag ttatccgtac aggacatcca tccgggcgaa aaggccggag acaaagaaga
    51421 ggcgattcgc caggtcgctg cggcgctggt gcaggccggt aatgtagcag aaggctacgt
    51481 caatggcatg ctggcgcgcg aacagcaaac ctcaacgttc ctcggcaatg gtattgctat
    51541 tccacacggc actaccgaca cccgcgatca ggtgctgaaa accggcgttc aggtatttca
    51601 gttcccggaa ggcgtcacct ggggtgacgg tcaggtagcg tacgtggcaa tcggtattgc
    51661 tgccagctcg gatgaacatc tgggcctgct acgccagctg acccacgtac tgagcgatga
    51721 ttccgttgct gaacaactga agtcagcaac aacagcagaa gaacttcgcg cattactgat
    51781 gggcgaaaag cagagtgagc agctgaagct cgacaacgaa atgctgacgc tggatatcgt
    51841 cgccagcgat ctgctgactc ttcaggcgct gaacgctgcg cgtctgaaag aggcgagggc
    51901 agttgacgcc actttcgtca ccaaagccat caatgaacaa ccgctgaacc tcggacaggg
    51961 tatctggctg agcgatagcg ccgaaggcaa tctgcgtagc gcgattgcgg taagccgtgc
    52021 ggcaaatgct tttgatgtgg acggcgaaac ggcagccatg ctggtgagtg tggcgatgaa
    52081 tgacgatcag cccatcgcgg ttcttaagcg tctcgctgat ttattgctcg acaataaagc
    52141 tgaccgcttg ctgaaagcgg atgcggcaac gttgctggcg ctgctgacca gcgatgatgc
    52201 gccgaccgac gacgtgttaa gcgcggagtt tgtggtgcgc aatgaacacg gcctgcatgc
    52261 tcgtccaggt accatgctgg tcaataccat taaacaattt aacagtgata ttaccgtgac
    52321 aaaccttgat ggtaccggca aaccggcaaa cggacgtagt ctgatgaaag ttgtggcact
    52381 tggcgttaag aaaggtcatc gcctacgctt taccgcccag ggtgcagatg ctgaacaggc
    52441 gctgaaagca atcggcgacg ctatcgctgc tggtcttggg gagggcgcat aatgagcaga
    52501 cgtgttgcta ctatcaccct taatccggct tatgaccttg ttggtttctg cccggaaatt
    52561 gaacgcggcg aagtgaacct ggtgaaaacc accggtctgc atgcggcggg taaaggcatc
    52621 aacgtggcca aagtattaaa agacctggga attgatgtca ccgttggcgg cttcctgggt
    52681 aaagacaatc aggatggttt tcagcaactg ttcagcgagc tgggcattgc caaccgtttc
    52741 caggttgtac aggggcgcac ccgaattaac gttaagctga cggaaaaaga cggcgaagtg
    52801 accgacttca acttctcggg ttttgaagtc acccccgccg actgggaacg ctttgtgact
    52861 gattctctga gctggctcgg tcagttcgat atggtctgtg tcagcggaag cttaccgtca
    52921 ggcgtcagcc cggaagcgtt caccgactgg atgactcgcc tgcgtagtca gtgtccttgc
    52981 attatctttg atagtagccg tgaagcgtta gtagcaggtt tgaaagcggc accgtggctg
    53041 gtgaaaccta accgccgcga gttggaaatc tgggcaggcc gtaaactgcc tgaaatgaaa
    53101 gatgtgattg aagctgcaca tgcgctacgt gaacaaggta ttgcgcatgt tgttatttca
    53161 ctgggtgccg aaggcgcgct ttgggttaat gcctccggcg aatggatcgc caaaccaccg
    53221 tcagtcgatg tcgtaagcac cgttggcgca ggggattcta tggttggtgg cctgatttat
    53281 ggcttgctga tgcgtgaatc cagtgaacac acactgcgtc tggcgacagc tgttgcagcc
    53341 ctggcggtaa gtcaaagcaa tgtgggtatt accgatcgtc cgcagttggc cgcaatgatg
    53401 gcgcgcgtcg acttacaacc ttttaactga cagcaggaga ggcataatga aaacgctgct
    53461 gattattgac gctaatctcg gtcaggcacg cgcctatatg gcgaagaccc tgctgggcgc
    53521 ggcggcgcga aaagcaaaac tggaaatcat cgacaatccg aacgacgcag aaatggcgat
    53581 tgttctcggt gattccatcc cgaatgacag cgcgctgaac ggtaaaaatg tctggctggg
    53641 tgatatttcc cgggcagttg cgcaccctga gctgttcctg agtgaagcca aaggccatgc
    53701 gaaaccttac actgcgccgg tcactgcgac agcaccggtt gccgccagcg gtccgaaacg
    53761 cgtagttgcg gtgactgctt gcccgactgg cgtagcacac acctttatgg cggctgaagc
    53821 cattgaaacc gaagcgaaaa aacgtggctg gtgggtgaaa gttgaaaccc gtggttctgt
    53881 tggcgcgggt aatgcaatca ctcccgagga agtagccgca gcggatctgg tgattgtggc
    53941 ggcagatatc gaagtggatc tggcgaaatt tgctggtaaa ccgatgtatc gtacctctac
    54001 cggtctggcg ctgaagaaaa ccgcgcagga actggataaa gcggttgctg aagcaacgcc
    54061 gtatgaaccg gcgggcaaaa ctcaaacggc gaccactgaa ggtaagaaag agagtgcagg
    54121 cgcttatcgt cacttgctaa cgggcgtctc ttacatgctg ccgatggtcg ttgctggtgg
    54181 tctgtgtatc gcgctttctt ttgcttttgg tatcgaagcg tttaaagagc cgggtacgtt
    54241 ggcagcggcg ctgatgcaga ttggtggtgg ttcagccttt gcgctgatgg tgccggtact
    54301 ggcaggttat attgcctttt ccattgccga tcgtccgggc ctcacgccgg gtctgattgg
    54361 cggtatgctg gcggtcagca ccggttctgg cttcattggt ggtattattg cgggcttcct
    54421 ggctggttac attgcgaagt taatcagtac gcaattgaaa ctgccacaga gtatggaggc
    54481 gctgaaaccg atcctgatca ttccgctaat ttccagtctg gtggtcggtc tggcgatgat
    54541 ctacctgatc ggtaaaccgg ttgctggcat tctcgaaggg ttgactcact ggctgcagac
    54601 catggggact gcgaatgcgg ttctgctggg ggcgatcctc ggtggcatga tgtgtactga
    54661 catgggcggt ccggtaaata aagcagcgta cgcattcggt gtgggtctgc tgagtactca
    54721 aacctatggc ccgatggcgg cgattatggc ggcaggtatg gtgccaccgc tggcaatggg
    54781 cctggcaaca atggtggcgc gtcgcaaatt cgacaaagcg cagcaggaag gtggcaaagc
    54841 cgctctggta ctgggactgt gctttatttc ggaaggtgca attccgtttg ctgctcgtga
    54901 tccgatgcgt gtgctgccgt gctgtatcgt gggtggggcg ctgactggcg caatctcaat
    54961 ggcgattggt gcgaaactga tggcaccgca cggtggtctg tttgttctgc tgatccctgg
    55021 cgctattacg ccggtattgg gttacctggt agcaattatt gccggtacgc tggtggcggg
    55081 tttggcctat gccttcctga aacgtccgga agtggacgca gtagcgaaag cagcgtaata
    55141 aaaggtgtgt tacagggcag aaatttcctg ccctgtatca ttggatgagc gcaaagggaa
    55201 tgaccggttt gtaaatgtaa ttttatgaat ttaaatggtt ttatctttca gtagaaacct
    55261 gaaacgttca tcaaacacta aggttatttc ctcaagggca gattattttc tgcccttttt
    55321 tagtgttcct gttctgtgca tcccgtcaca aattcctcgc ttttgtaatt caggcattta
    55381 atcgcatttt gtgagcctgc tcaaatctgc ccattttgcc tctctggcgg taatttactt
    55441 tacgtaaaac atgcatacta tgagcacatg tttaaatatg aacacatgtt taaaccgtct
    55501 ttgcagtatc tatattcttg tggattgaca gggttggtaa ttttttacca gaggacgtgc
    55561 tatgcgcgaa aaggattatg tcgtaattat aggttcggcg aatattgatg tcgccggata
    55621 ttcacatgaa tcattaaatt atgcggattc aaatccaggt aaaataaaat ttacgcctgg
    55681 tggagtaggg cgcaatattg cacaaaacct ggcgttgctg ggtaacaaag cctggctact
    55741 gagcgcagta ggcagtgatt tttatggtca atcgctgcta acgcaaacca atcaatctgg
    55801 cgtttatgtc gataaatgcc tgattgtgcc gggagaaaat acgtctagtt atttatcatt
    55861 actcgataat accggtgaaa tgctggttgc tataaatgac atgaatatta gcaacgctat
    55921 tacagctgaa tatctcgcac agcaccgtga atttattcag agggcaaagg tcattgtcgc
    55981 agactgtaat atcagtgaag aggcactggc atggattctg gataatgccg ccaacgtacc
    56041 cgtatttgtc gatccggttt cagcgtggaa atgtgtcaaa gtgcgcgacc gtctaaatca
    56101 gatccacact ctcaagccaa accgccttga agcggaaacc ctgagtggga ttgcgctgtc
    56161 agggcgtgac gatgtggcaa aagttgctgc ctggttccat caacatggcc tgaaccgact
    56221 ggtattgagc atgggcggcg atggcgttta ttacagcgat atcagcggtg aaaatggctg
    56281 gtctgcgccg atcaaaacca atgttattaa tgttaccgga gcgggcgatg ccatgatggc
    56341 gggacttgct tcgtgttggg tagacggaat gccgtttgcc gaatctgttc gtttcgcaca
    56401 gggatgttcg tcaatggcgc tctcctgtga atacaccaat aaccccgatt tatcgattgc
    56461 caacgttata tcgttagtgg agaacgcaga atgtctgaat taaaaatttc ccctgaatta
    56521 ttacaaattt ccccggaagt gcaggacgct ttaaaaaaca aaaaaccggt tgtggcgctg
    56581 gaatcgacca ttatttctca cgggatgccg ttcccacaaa atgcccagac cgcaattgaa
    56641 gtagaagaaa ctattcgtaa acagggcgca gtacctgcca ctatcgccat tattggcggc
    56701 gtgatgaaag tgggtttaag caaagaagaa attgaattac tgggtcgtga agggcataac
    56761 gtgaccaaag ttagtcgtcg cgatttacct tttgttgttg ccgccggaaa aaatggcgca
    56821 accactgtgg cttcaacgat gattattgcg gcgcttgccg gaattaaagt atttgccacc
    56881 gggggaattg gtggtgtgca tcgcggggcg gaacatacct tcgatatttc tgccgatttg
    56941 caagaactgg caaatactaa tgtcaccgtt gtttgtgccg gggcgaaatc tattctcgat
    57001 ttaggattaa ccactgagta tttagaaacc ttcggtgtgc cgttaattgg ctatcagact
    57061 aaagcgctgc ctgcgttttt ctgccgtacc agctcgtttg acgtcagcat tcgtctcgac
    57121 agcgccagtg aaattgcccg tgcaatggcg gtgaaatggc aaagcgggct gaacggtggc
    57181 ctcgtggtag cgaacccgat cccggaacag tttgcgatgc cggaggaatc tatcaatgca
    57241 gccatagatc aagccgtcgc cgaagccgaa gagcagggcg ttattggtaa agaaagtaca
    57301 ccgttcctgc tggcgcgcgt tgctgagctg accggcggtg acagcctgaa atccaatatc
    57361 cagctggtgt tcaacaacgc cattctggcg agtgaaattg ccaaagaata ccagcgtctc
    57421 gcgggttaat ttctgacggg cagaaagccg tcgcccgtac tcatcaagca taccaaaacc
    57481 tggcgctgac gccgggttta ctggcatgaa gggaaaatca ttatggatat aatgagaagt
    57541 gttgtgggga tggtggtgtt actggcaata gcatttctgt tgtcagtgaa taaaaagagc
    57601 atcagtttgc gcacggttgg agccgcactg ctgctgcaaa tcgctattgg tggcatcatg
    57661 ctctacttcc caccgggaaa gtgggcagta gaacaggcgg cattaggcgt tcataaagtg
    57721 atgtcttaca gtgatgccgg tagcgccttc atttttggtt cgctggttgg gccgaaaatg
    57781 gatgtcctgt ttgacggtgc gggttttatc ttcgcctttc gcgtacttcc ggcgattatt
    57841 ttcgttactg cgctcatcag tctgctgtac tacattggcg tgatggggct gctgattcgc
    57901 atccttggca gcattttcca gaaagctctt aacatcagca aaatcgaatc ttttgtcgca
    57961 gtcacaacta ttttcctcgg gcaaaatgag atcccggcga tcgttaaacc gtttatcgat
    58021 cgcatgaatc gcaacgagtt gtttaccgca atttgtagcg ggatggcgtc cattgctggt
    58081 tcgatgatga ttggttatgc cggaatgggc gtaccaattg actacctgtt agcggcatcg
    58141 ctgatggcga tcccaggtgg tattttgttt gcacgtattc ttagcccggc caccgagcct
    58201 tcacaggtca catttgaaaa tctgtcgttc agcgaaacgc cgccaaaaag ctttatcgaa
    58261 gcggcggcga gcggtgcgat gaccgggctg aaaatcgccg ctggtgtagc gacggtggta
    58321 atggcgtttg tcgcaattat tgcgctgatc aacggcatta tcggcggaat tggtggctgg
    58381 tttggtttcg ccaatgcctc tctggaaagt atttttggct atgtgctggc accgctggcg
    58441 tggatcatgg gtgtggactg gagtgatgcc aatcttgcgg gtagcctgat tgggcagaaa
    58501 ctggcgatta atgagtttgt tgcttatctc aatttctcgc cctatctgca aacgagcggc
    58561 acgctggatg tcaagaccat tgccattatc tcgttcgccc tctgtgggtt tgcaaacttt
    58621 ggttctatcg gtgttgtcgt tggcgcattt tcggctattt cgccaaaacg tgctccagaa
    58681 atcgcccagc ttggtttacg ggcgctggca gcagcaacgc tttccaacct gatgagtgcg
    58741 actattgccg ggttctttat tggtctggcg taatgctcga tttgtagacc ggataaaacg
    58801 tattaagcgt tgcatccggc aatcgcgcta ttgcaaataa tttttactgc atcatcccgg
    58861 agaatttatt ctccgggtcc atctccagcg ccagtcctga caactgcttt ctgtttttaa
    58921 tgagataccc tttcttgctt ttagttaata aaccgtcgtg aatgaattgc gcgagaacat
    58981 ataaaagatg tcgataagaa acgcctaaat actctgccgc ttgcgtatgc ttttcgtgat
    59041 aaagatcacc ttcctgcgag agtaatataa aagctgccag gcgattaact aacggaaatg
    59101 attgattctg agttaaagaa acaatattac gataattttt atgacttaag gtgacgcaga
    59161 gttttcgtaa aaatagcgtg tcgtttaata acagcgggcg gtaatgtttc atagggagcg
    59221 caaggcacca acactcttca atagcctgca ccgcacgcgg ttcatggtct ttgtcgatta
    59281 actcaatctc gccaataaaa cagggggcgg caaagaaatc gatcagcgac acgcgaccat
    59341 tagctagcgt ggcgtaaagc ctggcgcggc ctcgcgtcag gtaaaacagc caggacggct
    59401 gttgcccttc ctgcacaatg tagtcacgcg ctaaaaaatg aaacagccgc gtatcggctg
    59461 aaacatccgt taaaaagcaa tccttaaacg cggattcact catcaattgc tgcttaagat
    59521 cattattgtg gatttctttc acgatgttta tccgctatga gatttctcat atttagcata
    59581 cgtacgcggt tggtaagatt caatcatcgg cccggtcgaa ttttctttgc tgtagaaaaa
    59641 ataacgcaac tggaaacaga ggaaataaac aatggaaaag agaaaaatta ttctggattg
    59701 tgatccgggt catgatgatg ctattgcaat gatgatggca gcgaaacatc cggcaataga
    59761 tttattaggc atcactattg tagcgggtaa tcagacgctt gataaaacat taattaatgg
    59821 cctgaatgtt tgccagaaac tggagattaa tgttccggtt tatgcgggga tgccgcaacc
    59881 cattatgcgt aaacaaatcg ttgccgataa tattcacggt gaaaccggac tggatggccc
    59941 ggtattcgag ccgctgaccc gccaggcaga aagtacccac gcggtgaaat acatcattga
    60001 taccctgatg gcaagcgatg gcgatatcac tctggtgccg gttggtccgc tttcaaatat
    60061 cgcggtggca atgcgtatgc aacccgcgat cctgcccaaa atccgtgaaa ttgtgctaat
    60121 gggcggcgct tacggtacag gcaacttcac gccatctgcc gagttcaaca tctttgctga
    60181 tccggaagca gcccgcgtgg tgttcacctc cggcgttcca ttagtgatga tgggcctcga
    60241 tctcaccaac cagaccgttt gcaccccgga cgtgattgca cggatggaaa gggcaggcgg
    60301 ccctgccgga gagctgttca gcgacatcat gaacttcact ctcaaaacgc agttcgaaaa
    60361 ctacggcctg gctggcggcc cggtgcacga cgccacctgc atcggttatc tgattaaccc
    60421 tgatggcatt aaaacacagg agatgtacgt cgaagtggac gtcaacagtg gcccttgcta
    60481 tgggcgtacc gtctgcgacg agctgggcgt tcttggcaag cccgctaata ccaaagtcgg
    60541 catcactatt gataccgact ggttctgggg cttagtcgaa gagtgcgtgc gcggctacat
    60601 caaaacccat taagtgttga taacaagccg ggtcagtatc gcaagatcca agcccggcta
    60661 tttctgatgg gcagaaagcc gtcgcccaat atgacatgaa gagataataa ctatggatgt
    60721 catgagaagt gttctgggaa tggtggtatt gctggcaatt gcatttttgc tgtcagtaaa
    60781 caagaagaag atcagcctgc gtaccgttgg cgcggcgtta gtgttacagg ttgtgattgg
    60841 cgggattatg ctttggttac cgccagggcg ttgggtcgct gaaaaagtcg cttttggcgt
    60901 gcataaagtg atggcgtaca gcgacgcggg tagcgcattt atcttcggtt cgctggtcgg
    60961 gccgaaaatg gacacgctgt ttgatggcgc aggatttatc tttggtttca gggtattacc
    61021 ggcaattatc ttcgtcactg cactggtgag tattctctac tacatcggtg tgatggggat
    61081 tttaattcgc attctcggcg gtatcttcca gaaagcgtta aatatcagca agattgagtc
    61141 attcgtcgcg gtcaccacca ttttcctcgg gcaaaacgaa attccggcga tcgtcaaacc
    61201 ctttatcgat cgtctgaatc gcaatgaatt atttacagcg atttgtagtg gcatggcctc
    61261 gattgctggt tcgacaatga ttggttacgc cgccctgggc gtacctgtgg aatatttgct
    61321 ggcggcatcg ttaatggcga tcccaggcgg gatcttgttt gcccgcctgt taagcccggc
    61381 tacggaatct tcgcaggttt cctttaataa cctctctttc accgaaacac cgccaaaaag
    61441 cattattgaa gccgctgcga caggggcaat gaccgggctg aaaatcgccg ccggtgtagc
    61501 gacagttgtt atggcatttg tcgccatcat tgcgttaatt aatggtatta tcggcggcgt
    61561 tggcggctgg tttggttttg cacatgcctc gctggagtcc attttaggtt acctgttggc
    61621 cccattggcg tgggtgatgg gggttgactg gagtgatgca aatcttgccg ggagtttgat
    61681 tgggcagaag ctggcgatca atgaatttgt cgcttatctc aatttctcgc catatctgca
    61741 aacgggtggc actctggatg ctaaaaccgt ggcgattatt tctttcgcgt tgtgcggttt
    61801 cgctaacttt ggttctatcg gggtggtggt gggggcgttt tctgcggttg cgccacaccg
    61861 tgcgccggaa atcgcccaac ttggtttacg cgcgctggcg gcggcgacac tttctaacct
    61921 gatgagtgct actattgcag gattctttat tggtttagcg tagtgcgcaa tttatgagcc
    61981 ggataagatg cggtcaagcg tcgaatccgg caacattgtt acaccattgg cactaatgaa
    62041 agcgcgttat cggcagacag ggtagggttg tttaagctgc cgctggcgcg cgaaattgcc
    62101 gcgcaggcta ccgcaaaacg ggcgctgtcg cggaaactgt atccttcaag aaagctatat
    62161 accaggcccg ccataaaacc atcgtcagca ccaaaactgt ctactgtcgt atgcgctggc
    62221 gcggtcagca aaaattgttc tccatcctta tcgctgcaaa atacagattc atctttcaga
    62281 taaacaaaaa tgcgtttaac accttgttga tgcagcgaat ttacagctct gatgcgatcg
    62341 ttgtcgtctt taatcggttg cccccacaaa atttccagtt cattttgtgt tggtttcaga
    62401 gtgtggatcc ggctgtacca gttttttact ttgttcgctt taaattcgga aacggtatcg
    62461 acaaacatcg gaatttcatc agcaatggta aagacccact ccagggcttc aggtgtgaga
    62521 ttacaatcag ccagcaccac accagcatgg cgaatcaagt cccgtgaagt attgagcagt
    62581 tgaggcgtta gctgttgcag aatatgtgtg tcattgattg ccaggatagt ttcttcctgc
    62641 ttattagcga tagcgagata agttgcagtg ctgtgtccat gcagacgaat gcagtttgaa
    62701 acgttcacgc ccgcgcggcg cgtttcttcc agtaatgtct cgccgtaaaa atcgttgcca
    62761 atagctgata ttaaatgcac atcccgacca agcagagcca ggttatgcgc gatattacgt
    62821 ccgacgccgc ctgcggaaca gtgaacagaa cctggatgag aggctgcttg cgggtaacgg
    62881 atatccgcca ttccgcgaat atccatattg attgcaccca caaccacaca gtaatcctgc
    62941 tcggtaagga tatatccttt gcctttaatc aaacccttgc gcatcaaatc cataatatga
    63001 gcggcaacac gtgagcggct gatttgcaga atatcggcga tttcattctg ttgaatcagc
    63061 gggttacgcc gtaaaatttt aagaatctgc ttttccctgt cgttcatcga tcaggccacc
    63121 gctttttcag tttgttgcgc tttcagccag gcgatctctt ctgcccagat atccgggttg
    63181 atggtttcaa ggatcagcgg aatgccgtcg aaacggtcgt cctgcatgat ccagcggaac
    63241 gcatcatgac cgatattgcc ttcaccgagg ctatgatggc ggtcaacgcg gctgccaaag
    63301 gtgcttttcg catcgttaag gtgcatcccg cgcagatact taaagccaac aatacgggca
    63361 aaatccgcga acgttttctc gcattcggct ggagtacgca aatcataccc ggcagcgaaa
    63421 gcatggcagg tatcaatgca gacgccgaca cgggatttat cttccacgcc gtcgataatc
    63481 gccgcgagat gttcgaattt aaaccctaag ttactgccct gaccggcggt gttttcaatc
    63541 accgctgtta caccttgagt tttatccagc gcaatgttga tggattcggc aatacgcgca
    63601 aggcaatcct cttctgaaat ctgcatcaga tggctgccag ggtggaagtt gagcaaagaa
    63661 agccccagtt gttcgcaacg ctgcatttca tctataaagg catcgcgcga tttttccaga
    63721 gcttcagtga ccggatgtcc gaggttaatc agatagctgt cgtggggaag aatttgcgcc
    63781 gatgtgtagt gatatttttc acaggccgct ttgaattcat cgatggtttg cgtcgtgagc
    63841 ggtgcggcac gccactgacg ttggtttttg gtgaacaagg caaacgcggt tgcgtcgatt
    63901 tcggcggcgc gaattgcggc atttgccaga ccgccagcag cactaacgtg cgctccaatg
    63961 tatttcatgc gaggactcct gttaaacccg ctggaggaaa acggtaatga tagcgggtta
    64021 acaggagcaa gatgtagtgg tttatgcgat gacgctttga atcacatagt taatcgcacc
    64081 accaccaaca atcagccagg caaacagtac cagtgccatc agcagaggtt tcgccccggc
    64141 ttttttcagc gcgctgacgt gagtggtcag acccagcgcc gccatcgcca ttgccagcag
    64201 gaaggtatcc agcgttacca gcatgttcac cacgctctgc ggtaacaggt ggaacgagtt
    64261 aaagatggca acgacgatga acaagatggc aaaccacgga atagtgattt tgcttttctc
    64321 gccgctgttc gccccagaca gctgtttaac acgcgccgcc agcaggatga ggaacggagc
    64381 cagcatcatc acgcgcagca ttttggaaat aactgctgcg ttttccgcat ccgggctgat
    64441 ggcatgacct gccgccacca cctgcgccac ttcgtgcaca gtagaaccaa tgtagatacc
    64501 gaaagtttcc ggactaaacc attgagacat cagcggata
//
LOCUS       DAFATA010000014        63923 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFATA010000014 DAFATA010000000
VERSION     DAFATA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.014"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..183
                     /gene="rcnB"
                     /locus_tag="LGJ20_001256"
     CDS             <1..183
                     /gene="rcnB"
                     /locus_tag="LGJ20_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBK2385350.1"
                     /translation="DTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDT
                     DGKIIKAYDGEIFYHR"
     gene            complement(265..1299)
                     /locus_tag="LGJ20_001257"
     CDS             complement(265..1299)
                     /locus_tag="LGJ20_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBK2385351.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRRSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(1313..3793)
                     /locus_tag="LGJ20_001258"
     CDS             complement(1313..3793)
                     /locus_tag="LGJ20_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2385352.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(3809..4483)
                     /locus_tag="LGJ20_001259"
     CDS             complement(3809..4483)
                     /locus_tag="LGJ20_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBK2385353.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(4564..5106)
                     /locus_tag="LGJ20_001260"
     CDS             complement(4564..5106)
                     /locus_tag="LGJ20_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2385354.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            complement(5399..5680)
                     /locus_tag="LGJ20_001261"
     CDS             complement(5399..5680)
                     /locus_tag="LGJ20_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBK2385355.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(5943..7052)
                     /gene="apbC"
                     /locus_tag="LGJ20_001262"
     CDS             complement(5943..7052)
                     /gene="apbC"
                     /locus_tag="LGJ20_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBK2385356.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            7184..9217
                     /gene="metG"
                     /locus_tag="LGJ20_001263"
     CDS             7184..9217
                     /gene="metG"
                     /locus_tag="LGJ20_001263"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK2385357.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            9358..13152
                     /locus_tag="LGJ20_001264"
     CDS             9358..13152
                     /locus_tag="LGJ20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBK2385358.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKVNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPRLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            13162..16794
                     /locus_tag="LGJ20_001265"
     CDS             13162..16794
                     /locus_tag="LGJ20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBK2385359.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     MMQWLSTQARAVLNSYLSAPPKPVIDSTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGERERLAATEPARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPIARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     IFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPVKDNWAFLALSLFGDESTARDLTTQILGWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     AYPANEPRCLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLARCTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            16855..17172
                     /locus_tag="LGJ20_001266"
     CDS             16855..17172
                     /locus_tag="LGJ20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385360.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            18412..19500
                     /locus_tag="LGJ20_001267"
     CDS             18412..19500
                     /locus_tag="LGJ20_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK2385361.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            19511..21790
                     /locus_tag="LGJ20_001268"
     CDS             19511..21790
                     /locus_tag="LGJ20_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385362.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            21783..22919
                     /locus_tag="LGJ20_001269"
     CDS             21783..22919
                     /locus_tag="LGJ20_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK2385363.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            22916..24916
                     /locus_tag="LGJ20_001270"
     CDS             22916..24916
                     /locus_tag="LGJ20_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBK2385364.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            25041..25502
                     /locus_tag="LGJ20_001271"
     CDS             25041..25502
                     /locus_tag="LGJ20_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBK2385365.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(25543..26013)
                     /locus_tag="LGJ20_001272"
     CDS             complement(25543..26013)
                     /locus_tag="LGJ20_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBK2385366.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(26060..26779)
                     /gene="yehT"
                     /locus_tag="LGJ20_001273"
     CDS             complement(26060..26779)
                     /gene="yehT"
                     /locus_tag="LGJ20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBK2385367.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(26776..28461)
                     /gene="btsS"
                     /locus_tag="LGJ20_001274"
     CDS             complement(26776..28461)
                     /gene="btsS"
                     /locus_tag="LGJ20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBK2385368.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            28683..29414
                     /gene="mlrA"
                     /locus_tag="LGJ20_001275"
     CDS             28683..29414
                     /gene="mlrA"
                     /locus_tag="LGJ20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBK2385369.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            29474..29581
                     /locus_tag="LGJ20_001276"
     CDS             29474..29581
                     /locus_tag="LGJ20_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBK2385370.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(29562..30293)
                     /gene="yehW"
                     /locus_tag="LGJ20_001277"
     CDS             complement(29562..30293)
                     /gene="yehW"
                     /locus_tag="LGJ20_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBK2385371.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(30298..31224)
                     /gene="yehX"
                     /locus_tag="LGJ20_001278"
     CDS             complement(30298..31224)
                     /gene="yehX"
                     /locus_tag="LGJ20_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBK2385372.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQSCGTLHFQDLLEEA"
     gene            complement(31217..32374)
                     /gene="yehY"
                     /locus_tag="LGJ20_001279"
     CDS             complement(31217..32374)
                     /gene="yehY"
                     /locus_tag="LGJ20_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBK2385373.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGFGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(32381..33298)
                     /gene="osmF"
                     /locus_tag="LGJ20_001280"
     CDS             complement(32381..33298)
                     /gene="osmF"
                     /locus_tag="LGJ20_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBK2385374.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(33509..35806)
                     /gene="bglX"
                     /locus_tag="LGJ20_001281"
     CDS             complement(33509..35806)
                     /gene="bglX"
                     /locus_tag="LGJ20_001281"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBK2385375.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            36002..37717
                     /gene="dld"
                     /locus_tag="LGJ20_001282"
     CDS             36002..37717
                     /gene="dld"
                     /locus_tag="LGJ20_001282"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBK2385376.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(37755..38684)
                     /gene="pbpG"
                     /locus_tag="LGJ20_001283"
     CDS             complement(37755..38684)
                     /gene="pbpG"
                     /locus_tag="LGJ20_001283"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBK2385377.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAGQTAQND"
     gene            complement(38858..39445)
                     /locus_tag="LGJ20_001284"
     CDS             complement(38858..39445)
                     /locus_tag="LGJ20_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBK2385378.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            39615..40193
                     /locus_tag="LGJ20_001285"
     CDS             39615..40193
                     /locus_tag="LGJ20_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2385379.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(40323..41084)
                     /locus_tag="LGJ20_001286"
     CDS             complement(40323..41084)
                     /locus_tag="LGJ20_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2385380.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(41137..42573)
                     /gene="mdtQ"
                     /locus_tag="LGJ20_001287"
     CDS             complement(41137..42573)
                     /gene="mdtQ"
                     /locus_tag="LGJ20_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBK2385381.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            42797..42880
                     /gene="yohP"
                     /locus_tag="LGJ20_001288"
     CDS             42797..42880
                     /gene="yohP"
                     /locus_tag="LGJ20_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBK2385382.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(43253..44200)
                     /gene="dusC"
                     /locus_tag="LGJ20_001289"
     CDS             complement(43253..44200)
                     /gene="dusC"
                     /locus_tag="LGJ20_001289"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBK2385383.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            44439..44837
                     /locus_tag="LGJ20_001290"
     CDS             44439..44837
                     /locus_tag="LGJ20_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK2385384.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            44834..45529
                     /locus_tag="LGJ20_001291"
     CDS             44834..45529
                     /locus_tag="LGJ20_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBK2385385.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            45659..46543
                     /gene="cdd"
                     /locus_tag="LGJ20_001292"
     CDS             45659..46543
                     /gene="cdd"
                     /locus_tag="LGJ20_001292"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK2385386.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAENADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            46693..47412
                     /gene="sanA"
                     /locus_tag="LGJ20_001293"
     CDS             46693..47412
                     /gene="sanA"
                     /locus_tag="LGJ20_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBK2385387.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            47415..47654
                     /locus_tag="LGJ20_001294"
     CDS             47415..47654
                     /locus_tag="LGJ20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBK2385388.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            47973..49211
                     /gene="preT"
                     /locus_tag="LGJ20_001295"
     CDS             47973..49211
                     /gene="preT"
                     /locus_tag="LGJ20_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBK2385389.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            49205..50440
                     /gene="preA"
                     /locus_tag="LGJ20_001296"
     CDS             49205..50440
                     /gene="preA"
                     /locus_tag="LGJ20_001296"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBK2385390.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(50511..51521)
                     /gene="mglC"
                     /locus_tag="LGJ20_001297"
     CDS             complement(50511..51521)
                     /gene="mglC"
                     /locus_tag="LGJ20_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBK2385391.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(51537..53057)
                     /gene="mglA"
                     /locus_tag="LGJ20_001298"
     CDS             complement(51537..53057)
                     /gene="mglA"
                     /locus_tag="LGJ20_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBK2385392.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(53118..54116)
                     /gene="mglB"
                     /locus_tag="LGJ20_001299"
     CDS             complement(53118..54116)
                     /gene="mglB"
                     /locus_tag="LGJ20_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBK2385393.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(54396..55436)
                     /gene="galS"
                     /locus_tag="LGJ20_001300"
     CDS             complement(54396..55436)
                     /gene="galS"
                     /locus_tag="LGJ20_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBK2385394.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(55578..56735)
                     /locus_tag="LGJ20_001301"
     CDS             complement(55578..56735)
                     /locus_tag="LGJ20_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBK2385395.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(56752..57420)
                     /gene="folE"
                     /locus_tag="LGJ20_001302"
     CDS             complement(56752..57420)
                     /gene="folE"
                     /locus_tag="LGJ20_001302"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBK2385396.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            57678..58514
                     /gene="yeiG"
                     /locus_tag="LGJ20_001303"
     CDS             57678..58514
                     /gene="yeiG"
                     /locus_tag="LGJ20_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBK2385397.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(58546..60537)
                     /gene="cirA"
                     /locus_tag="LGJ20_001304"
     CDS             complement(58546..60537)
                     /gene="cirA"
                     /locus_tag="LGJ20_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBK2385398.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNAVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(60830..62299)
                     /gene="lysP"
                     /locus_tag="LGJ20_001305"
     CDS             complement(60830..62299)
                     /gene="lysP"
                     /locus_tag="LGJ20_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBK2385399.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(62504..63385)
                     /locus_tag="LGJ20_001306"
     CDS             complement(62504..63385)
                     /locus_tag="LGJ20_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2385400.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            63484..>63923
                     /locus_tag="LGJ20_001307"
     CDS             63484..>63923
                     /locus_tag="LGJ20_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK2385401.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAA"
BASE COUNT        15951 a        16716 c        15636 g        15620 t
ORIGIN      
        1 gataccgtcc cagagatgta ccgcaccgat gagtacaaca ttaagcagtg gcagttacgt
       61 aacctgcccg cgcctgatgc cgggacgcac tggacctata tgggtggcgc gtacgtgttg
      121 atcagcgaca ccgacggtaa aatcattaaa gcctacgacg gtgagatttt ttatcatcgc
      181 taaaaaaagc cccctcatca tgagggggaa atgcagacac cttgttattt tttattatta
      241 gccacttgct cgtcttgctt gttattagtc gtatttcacg ttgattaatg cggttgcctc
      301 cagtgcgcca gatttaactt tgtttgtatc gtagacgtag taactggcag ttatcggaat
      361 tgaaaccgtt gtatctgtaa gttttgactt tgccgcaatt tcttttaaat ttgtgtatcc
      421 tttactgctg ttagtgatat ttaaaagtgt gactgacgaa ccattacttt caagaataaa
      481 tcctacgcca ttatcttccg cacctttcag tattgtgttg ctgttggtta acaagtcaga
      541 acttgataaa tacacctgca ttttccgtga tgtttgattt tgttgaccta ataaattttt
      601 acaacgcaga ataatatccc cttgtttact atttattgtt gctttgttac ctgtggctgg
      661 taattgtgaa acgggaatac tgtctaaagt aattaataaa tcgtccggtt tgcaggttgt
      721 ggttatcggg ttgtatgaca atgtcagatt aaaggaatag acacctgcat tttgtgccaa
      781 tgcatttctg tagctattta cacatgcatc ccatgacttg ccacctaata aacacagtag
      841 aaattgtact gcggcactcc tgcgtgtcga tgatgaagca ctggataatg tcgcaactcc
      901 attaatcatc actgaaccgc cgccccccgc agaaacattc acttttgcat tactggccgc
      961 aggtgtcacc gttaccgaat aaccactggt gaaactggtc tgattatttg ataacgtgat
     1021 gttgttttta ttggtccatt caaaatgtaa atttaattta atggtatcgt tataaagtcc
     1081 aacaataata tccgacgggc tcataggggt gatagtattt gtactcacgc atgacgaatc
     1141 attaaaatta tgctcatatg tcgccatttg tgtttgagta agtgcaaaat ccccctgaac
     1201 aaccaaattg tgctgaacag ataactcact ataacatgcc gcatagctca caggcataac
     1261 catcatcatt actataaata acattatacg aatattcata accacctgac tattactggc
     1321 aaatatagtt tctactttca tcaatctctt taccgaaggt gattgtgcat gaaagtcctt
     1381 gttgcttatc aattgctaca ttaaccgatg gcggtacttc attggtgcga ataaataact
     1441 ggcttccctg accgacaacg ccaatattat gaccatggat atcattgact tcataaccaa
     1501 acattaatgg ttgcccatcc gctcttaacg cttttataaa ccagggcttg cgctgatcgg
     1561 tatcaaaatt aaccagtaca accgcgccgc gataaggggc ggcaattttc cggttgccac
     1621 gtaattctgc ttcgctatcg ctttgcgaca catccagcat caggtgattt tcccgataag
     1681 gtgtcattcc gtcgtatacc accactccat tacggtttgt tgtgcgatat ttttgcccat
     1741 tgacataagc atctttaatt cctggcgcat tcatcacagc aaacgtttcg gaaagacggt
     1801 tcgccagatt aacgcccccc gaccaggcga caatgccccc tgaaacactg gctccagcct
     1861 gtcgataagt actcgactga ctataactgc cattcactgt cgcaaccggc gcgttccagg
     1921 tcaaattcgc cccagctgtc gtttcatttc cctgattctg ataactcagg ttgaccccat
     1981 agttaaactg atcccggctt ccaacggttc ctgataatcc cgtattattt gaggcaaacc
     2041 cctgatcatc aaacgtcgtt gagttagaca tatatatttg ccgacgaggc gtcgtaacgt
     2101 catcacccca atcaaaggga atcgatataa aaatattaaa acgtttctct tcatgatgat
     2161 tctcgtcata cgcctggctt gccgcgagga tatagcttat ccgccgccag ttattggaat
     2221 aactcaactg ataatcctta ctgctgccgc tacgccccca gtaatctcgc cataacgtac
     2281 ttaaggacac agacccccag ccttctggca atgactggct catattggca gagaagctat
     2341 ttttacggcc aaaatcgttc tggtaataat cggcaatgtc atagacatcg ttttcatcac
     2401 ggcgataatt atctttattg tttgcccaaa cgtgatcatt aaatgtccgg taatcacgcg
     2461 atgaataacg ccaggccgcc agaccaaaac gcgtcgacgt ttggctcaca aatttgttgt
     2521 aggcaatttg ataactttgc ccgtcaaaca catcgccgtt gtcttgttta ctatgcgact
     2581 tcgtggcatc gacggaaatg gcaccaaagc gtgtattcca gccagtcccc aaagtaaacg
     2641 cgtaataatt attcgcgacc atcgagccac catacagcgt caataaatta ttaaaaccat
     2701 actgataacc cgcctggaca aaatcacttt gtttgctcgc cccttcaata tggctacgac
     2761 ccgccgcaaa atcatatttc gatacgccgg gttgcagcat atttggcact gctgcataag
     2821 gcaccagata ggtggttacc gagccgtccg cctctttcac gctgacatca agatctgctc
     2881 caccaccggc caactgcaaa tctgtaatcg cgaacgggcc aggaggaacc tctttctgat
     2941 aaaccacaaa accgttctgt tcaatagtta ccagcgcatt actctgagca atcccctgca
     3001 cccgtggcgt aaaattttgt ttcgagttag gcaacatctg catatcacga aacaaccgca
     3061 caccgctgaa gcgaacagaa tcaaaaatat cgcttgatgt gtacatatca cccacgcgaa
     3121 gcgtgccgag aagttgggca aatccacgtt ccagatacag ggtattgctt ttccacaccc
     3181 ctggattgtt atttgtttta ctgaaactgg catcagaatg cagttgccac cccagtaaat
     3241 ttaacccgct gttaaaacgt acatatgtac tcttactatt acccgacgct ttatagtcac
     3301 tgtaatactg actcacataa taagaggtat aaaacgcatt aataccccgc tcccagtttt
     3361 ccggtggaac atagccactt tccagttctt ccacccaggc ctgcgggaca ctgaaatcga
     3421 gacgaaaaac cccgatatcc caggtatagc tcccaccctg aacaagttgc tcaaatgtta
     3481 aacattgctt accgctggcg aagttatcgc tattaatgcc taaccgcttg ataacttcta
     3541 ttgataaaca tgtttcttgc gggttgtctt taacaataat ctcatatttc ccgcgccatt
     3601 gcttattgac ataaatatcg atgtcatact gccccggtaa gggttgatta tcatcaagac
     3661 gaatatttgc aacctgctgg tctttcattc cacctatcat aaaatgggta tcaaaggttt
     3721 cttcagctgc ataagcttca atgccgagca ataacgctac gattgctgat gcaagtgggg
     3781 tcattctcaa cataggcagc tcctgcaatt aaattttgtc actaatatag ttgccgtggt
     3841 catcgataat ggtcagatac cagttatttg cattattact tttgacatta acactctgac
     3901 tttctaaggg ggcaatcata atagtttcat aattgacttt aacattatta gctttgacat
     3961 ccgaaatcgt cacccaatta gctgagtcat tttttatcac caatccattg ccactgcgat
     4021 ttaccagtaa ctttttaaag gtcgctttat ttaccggagc aatacccgct ggccggtaaa
     4081 acaacttaat tctgttttgc atcgcaaact tcagtgcatt tttgccttct tgctctggac
     4141 tatttggtgg aatatccaga acattcagat aaaaaatgct ttctttatta gtgggcagtt
     4201 tattcggcat aattttgatt tttacttgct gcccggaatt tgcccctatt tttgccactg
     4261 gtggcgttaa catgaaagga acctgaattt tttctggtgg taacgacgta tcgccatcat
     4321 caatccacgc ctgcagcagc gaagaacggt ttccctggtt catcaactgc accataactt
     4381 ctttattttc tgccgggtaa ataatgcgcg tcccgtagat aacaattccg gcatgtgcag
     4441 gaaggaccat agaaaaaatg agtaaagata ataatccttt catcttaatg cctcttaaat
     4501 taaaaagccg ccatggggat agcggccatg tttattacct ggagaaactt acaattttat
     4561 ttattaatcc tgcgccacac gcataaccag agaagataac acatcacctg attgcactga
     4621 gttatctgtc agtttcaggt attttgcttt aaacggaata gcatattgac cttccgctgt
     4681 tttataactc tcaatgttaa aaggtgcaga aacatccagg acatcgctgt tatcacctaa
     4741 atagagatag taacccacgg tatcgttgct actggctatc gccttatcgt taccggttat
     4801 tttatcgttg gtaatcaacg agagttttac accagcaact tctttcgggc agctaatttt
     4861 taacatgctg ctggttacac cgactgcatc atcataggta gaatatttag ctttaccttc
     4921 aaagacagtg gttggtacag tcggcatatt aatacttact gaatcaccac ccgcttcgaa
     4981 tttacacgga gattctgcaa tattcccctt aatagttaat gttccggtat caacgtctgc
     5041 agcaaatact ccagcgctca taaaaaaaga agaaaagaca gcggcagcaa taattgaacg
     5101 tttcataata tgttccttaa taaaatttat ttttggttgt taagagtctg tatctcttgg
     5161 aaacaaattt ctcacatcaa aaaagttgcc gcaatgtaaa cacattgttt tattgattta
     5221 aaacaaatta accattgcaa tataaataca aaaaatgttt aagagtatta actatttatc
     5281 gcatctatca attaatgtag atttatgtaa atggtattgt tgatatcaat aaaaaagccg
     5341 aaatcattta tataaatgat ttcggctttc taaatgcaat taaaatcagg ttgccaaatt
     5401 aatcgtagcg atttaacacg attcgacgtt tgctatcgct acctgcaaca accacttctg
     5461 tcgttacacc tttgacgggc gaagaaaaca gatgacgggt gtcaacatta tacgttaact
     5521 gaccacaatg ctgttgaggt tgaccattaa ccatagccat actgcatgtt gggggactga
     5581 tcctgccatt aatcgctaat gtggcagtct ctgaagagaa tgcgggagaa gccaatccat
     5641 aggcaagaaa aataattcct gacagccaat acttattcat tgaacgttat ccctgtagta
     5701 aaggttatgc ctggatagaa tgagtgcata acaaactata gctgtacatc cactacacag
     5761 ccacgaagga tgataatgaa gcattgcctg tatgatcaat cgactttgta gagtttcgga
     5821 cgaaggtccg cagaatattc gcagtattaa ataagtgttc attaatgcgg caaaaactaa
     5881 tacaccgcat caatgtaaca tctctgcggt gtatgaagaa taaccagaat ggttaatagg
     5941 cgttagaccg cgcggaagga aatctcgcct ggaatgactt caccctgcca gtagagctga
     6001 gctgcaacgc ggtcagccag ctggcggtag atagcggtaa attcgctctc cggacggctg
     6061 ataaccgttg gcgtgccttt atcgagatcc tcacgtaagg agatgtgtaa cggcatctgc
     6121 cccagcagct gtgtgtggta tttttcagcc agtttctcag caccaccggt accgaaaatc
     6181 ggttcatggt gaccacagtt actgcaaata tgcacactca tgttctcgac aataccaagc
     6241 accggaacct cgactttctc gaacatcaca atgccttttt tcgcatcgat caacgcgatg
     6301 tcctgcgggg tggtgaccac gactgcacca gttactggaa tgttttgcgc cagcgtcaat
     6361 tgaatgtcac cggtgcctgg cggcatatca agcaccagat agtcgagatc cggccacaag
     6421 gtttcctgca acatctgcat taacgccttg ctcgccatcg gtccacgcca caccatcgca
     6481 ttgtcgtcag tgaccagata accaattgaa ttggttgcca ggccatgaga cataatcggt
     6541 gccatgtggg taccgtccgg cgacgttgga cgctgatttt ctgcgcccag catggtggga
     6601 attgatgggc cgtagatatc ggcgtccaga atgccgactt tcgccccttc cgccgccagt
     6661 gccagcgcca ggtttaccgc cgtggaggat ttacccacgc cgcctttacc ggagctgacc
     6721 gcaatgatgt tcttcacacc attaataccc ggctgattct tcacgcgttt taaggtggcg
     6781 atattgtgcg acagtttcca gtcgatagct ttcgccccgg tgatacgcag cagttcggcg
     6841 ctacattgct cttttaactc ttcaaacgcg ctatgccaga cgaatggcat aaccagttcc
     6901 acatgcaacg tgtcgtccat ccaggcgaca tggtgcaacg ctttcagcgt ggtgaggtta
     6961 tgcttcaggg ttgggtgctg aaaattggcc agtgtcccgg cgaccattgc gcgcaaggct
     7021 tctggtgatt tggcctggga ttgttcgttc atcccgactc cttttttgta tagataaacc
     7081 atcagctgat agtttacctg aagaatatag agaagtactt acttaacatt ttcccatttg
     7141 gtactatcta accccttttc actattaaga agtaatgcct actatgactc aagtcgcgaa
     7201 gaaaattctg gtgacgtgcg cactgccgta cgctaacggc tcaatccacc tcggccatat
     7261 gctggagcac atccaggctg atgtctgggt ccgttaccag cgaatgcgcg gccacgaggt
     7321 caacttcatc tgcgccgacg atgcccacgg tacaccgatc atgctgaaag ctcagcagct
     7381 tggtatcacc ccggagcaga tgattggcga aatgagtcag gagcatcaga ctgatttcgc
     7441 aggctttaac atcagctatg acaactatca ctcgacgcac agcgaagaga accgccagtt
     7501 gtcagaactt atctactctc gcctgaaaga gaacggtttt attaaaaacc gcaccatctc
     7561 tcagctgtac gatccggaaa aaggcatgtt cctgccggac cgttttgtga aaggcacctg
     7621 cccgaaatgt aaatccccgg atcaatacgg cgataactgc gaagtctgcg gcgcgaccta
     7681 cagcccgact gaattgatcg agccgaaatc ggtggtttct ggcgctacgc cggtaatgcg
     7741 tgattctgaa cacttcttct ttgatctgcc ctctttcagc gaaatgttgc aggcatggac
     7801 ccgcagcggt gcgttgcagg agcaggtggc aaataaaatg caggagtggt ttgaatccgg
     7861 cctgcaacag tgggatatct cccgcgacgc gccatacttt ggttttgaaa ttccgaacgc
     7921 gccgggcaaa tatttctacg tctggctgga cgcaccgatt ggctacatgg gttctttcaa
     7981 gaatctgtgc gacaagcgcg gcgacagcgt aagcttcgat gaatactgga agaaagactc
     8041 caccgccgag ctgtaccact tcatcggtaa agatattgtt tacttccaca gcctgttctg
     8101 gcctgccatg ctggaaggca gcaacttccg caagccgacc aacctgtttg ttcatggcta
     8161 tgtgacggtg aacggcgcaa agatgtccaa gtctcgcggc acctttatta aagccagcac
     8221 ctggctgaat cattttgacg ctgacagcct gcgttactac tacactgcga aactctcttc
     8281 gcgcattgat gatatcgatc tcaacctgga ggatttcgtt cagcgtgtga atgccgatat
     8341 cgtgaacaaa gtggttaacc tggcctctcg taatgcgggc tttatcaaca agcgttttga
     8401 cggcgtgctg gcaagcgaat tggctgaccc gcagttgtac aaaaccttca ctgatgccgc
     8461 tgaagtgatt ggtgaagcgt gggaaagccg cgaatttggt aaagcagtgc gcgaaatcat
     8521 ggcgctggct gatctggcta accgctatgt cgacgagcag gctccgtggg tggtggcaaa
     8581 acaggaaggc cgcgatgccg acctgcaggc aatttgctca atgggcataa acctgttccg
     8641 cgtgctgatg acttacctga agccggtact gccgaaactg accgagcgcg cagaagcatt
     8701 cctcaataca gaactgacct gggatggtat ccagcaaccg ctgctgggcc acaaagtgaa
     8761 tccgttcaaa gcgctgtata accgcatcga tatgaagcag gttgaagcac tggtggaagc
     8821 gtctaaagaa gaagtgaaag cagctgccgc gccagttact ggcccactgg cagatgatcc
     8881 gattcaggaa accatcacct ttgacgactt cgcaaaagtt gacctgcgcg ttgcgctgat
     8941 cgaaaacgca gagtttgttg aaggttctga caaactgctg cgcctgacac tggatctcgg
     9001 cggtgaaaaa cgcaatgtct tctccggtat tcgttctgcc tacccggatc cgcaggcact
     9061 gatcgggcgt cacaccatta tggtggccaa cctggcaccg cgtaaaatgc gctttggtat
     9121 ctccgaaggc atggtaatgg ctgctggtcc tggcgggaaa gatatcttcc tgttaagccc
     9181 ggatgctggt gctaaaccag gccatcaggt gaaataatcc ccctttaagg cgctgcatcg
     9241 acagcgcctt ttctttataa attcctaaag ttgttttctt gcgattttgt ctctctctaa
     9301 cccgcataaa tactggtagc atctgcattc aactggataa aatcacaggg atgcagaatg
     9361 agacacttta tctatcagga cgaaaaatca cataaattct gggcagttga gcaacaggga
     9421 aacgagttgc atatcagttg gggaaaagtt ggcaccaaag ggcaaagcca gataaaaagt
     9481 ttttcagatg ctgcggcagc ggcaaaagcg gagcttaagc tgattgcgga gaaggtgaag
     9541 aaggggtatg tggagcaagc gaaggttaat tctttgcaac cttcccaaac ggtaacgggc
     9601 tctctcaagg tagcggactt atccaccatt attcaggaac aaccctcttt cgtagcagaa
     9661 acccgtgcgc ctgacaaaaa tacagatgct gttttaccgt ggctggcgaa agatattgct
     9721 gtcgtttttc cgcccgaagt tgtacacacc acgttaagtc atcgccgctt tcccggagtt
     9781 cctgttcagc aagcagacaa attgacccaa ttacgtcgct tagcctgtag tgtgtcgcaa
     9841 cgggataata aaacagccac atttgacttc agcgcctgtt ctttagaatg gcaaaacacc
     9901 gtcgcccagg cgatcagtca gatcgacggc ctgaaaacaa cacagctacc atcaccagta
     9961 atggctgtac tcacggcact tgaaatgaaa tgcacaagat ataaagtgcg tgaggatgtt
    10021 atggatcaga tcgtccagga aggcggtctg gaatatgcta ctgatgtaat aatacacctt
    10081 caacagattg atattaaatg ggattatgcg aataatgtca ttattattct accgtctggc
    10141 attgcacctg actacttgga gcaatattcc agatttgaat tacgcctacg taaacattta
    10201 tcactggcgg aagagtctct ctggcaaaaa tgtgcacaaa aacttattgc cgcaattcca
    10261 catattccag aatggcggca accattaatt gctttgttat tacccgaaaa accagaaatt
    10321 gcacatgaaa ttgcccagcg tctactgggg caaaaaaaat taccctcgct tgagtggtta
    10381 aaaatagtgg cgactgatga gcacattctt gcctcattag aaaaatatca cgagccatat
    10441 gccatttttg atgattacta ttgtggtgcg atatggtcag ccaccgtatt acaggagcaa
    10501 ggtgttgcag ccctgccccg atttgctccc tatgccgcaa gtgactactg cgccgatgtg
    10561 ttgcgtcata tcaatcatcc gttcgcatta acactgctta tacgtgtagc cgggcatact
    10621 aaacgctgtc acgatcggat gacgaaagcc attgctgcgt tcccacatgc agcaatggcg
    10681 gcactgacgg aacttcttgg gcaaaaagaa gagaacagtt ggcgcattat gctaatgaca
    10741 atgcttatct cacaaccagc actggcagaa caggtcattc cctggctctc gacacccgca
    10801 gttgccgtac tgaaatcatg ccagcaacaa ctgacacagc cctcaaacca tgccagcgcc
    10861 gatctactgc cagccgtagt agtctcccct ccctggcttt cgaaaaagaa aaaatcgccg
    10921 attccggtgc tggatttagc gccattaggc attgagccaa tctgttatct gacagaagaa
    10981 atcagtaatc aacttttggc gaaatatatc tggtattcaa aacacatcac ggttagccat
    11041 gaagaaagta ctaccaacct gttggcaagg atgggttttc aacgacggat cgctggtaca
    11101 tatattaaag ctcccgaagc ggtagttgag gcatggctaa atgaagatta ttcaacctta
    11161 ctaagtgaat ttaaggtgtt tcattcacct accgggcatt attggcagtt ggggattttg
    11221 acaacattgc cgctggagaa agcagtaaaa gcatggaatg cccttaccct atctccacat
    11281 accgataccg aatacgccat gttacatttt ggactcaaag ggttacctag gttagtaaac
    11341 tcacttgcac gctatccaca agaagccttg cccatcacga attacttcgc agcgagtgag
    11401 ctggctcctg ccgtcgcccg tgccttcaac aaactgaaaa cgctacgcga aaacgcccgt
    11461 agctggctgt tgaaataccc ggaacatgcc cttaccggcc tgctgcctgc agcgctcggc
    11521 aaagccggtg aagcacagga taacgcccgc gctgccttgc gtatgcttac cgaaaacggt
    11581 catcagccat tactgcaaga aatcgcccga cgttataacc agccggaagt aaccgatgcg
    11641 gtgaacgctc tgcttgcgct cgatccctta gataatcacc cgacaaaaat ccccactctt
    11701 ccggcctttt atcagccatc gctctggacg cgcccggtat taaaagcaaa tgcccaatca
    11761 ctgccagata gcgccctcct ccacctcggt gaaatgctcc gcttccctca ggaagaggct
    11821 ctgtatccgg gattattgca ggtgaaagac gtctgttccg ccgactcact ggcgggattt
    11881 gcctgggatc tgtttaccgc ctggcagacc gctggcgcgc cgtcgaaaga gagttgggcg
    11941 ttcactgcgt taggcgttct cggtaacgat gacaccgccc gcaaactgac gccattaata
    12001 cgcgcctggc ctggtgaatc ccagcataaa cgcgccaccg ttgggttgga tattctcgct
    12061 gctatcggta gtgatatcgc ccttatgcag cttaacggca tcgcccagaa actgaaattc
    12121 aaagcattac aggagcgggc aaaagaaaaa attgccgaca ttgccgagag ccgcgaactc
    12181 acggtggcgg agcttgaaga tcggttagca ccggatctcg gtctggatga taacggttcg
    12241 ctgctgctgg attttggccc acggcagttc accgtcagct ttgatgaaac cttaaaaccg
    12301 tttgtgcgtg atgtttccgg cagccgcctg aaagacctgc ccaaaccgaa caaaagcgat
    12361 gatgaatcgc ggtcgaacga tgcggttaac cgctacaaat tgctgaaaaa agatgcgcgt
    12421 accgtcgccg cccagcaggt agcaaggctg gaatccgcca tgtgcctgcg ccgccgctgg
    12481 tcgccggaaa acttccagct cttcctggtt gagcatccgc tggttcgcca cttaacccgc
    12541 cgtctgattt ggggcgttta tagcgccgaa aaccagctac tggcttgctt tcgcgtagca
    12601 gaagataaca gctacagcac cgctgacgat gatcttttca ccctgccgga aggcgatatc
    12661 tctatcggca ctcctcacgt tctggaaata tcaccaacgg atgctgccgc ctttggtcag
    12721 ctttttgccg actacgaact gctaccaccg ttccgccagc tcgaccgtaa cagctacgcc
    12781 ctgacagaag ccgagcgcaa tgccagtgaa ctgacccgct gggcaggcag aaaatgcccg
    12841 agtggtcggg tcatggggct ggcgaataaa ggctggataa agggcgaacc acaggatgga
    12901 ggctggatcg gatggatgat caaacctttg ggtcgctggt cgttaatcat ggaaatcgac
    12961 gaaggttttg cggtgggtat gtcaccagcc gaacttagcg ctgagcagct cttaagcaag
    13021 ctgtggttat gggaaggcaa agcggaaagc tatggctggg ggagtaattc aacacaggaa
    13081 gcgcagttct ccgtactcga tgccatcacc gccagcgagc taattaacga tattgaagcc
    13141 ctgtttgaat aaggaaagag catggacaag gaattaccgt ggctggcgga taacgcccaa
    13201 ctggaactga aatataaaaa aggcaaaaca ccactcagtc atcgcaaatg gccgggcgaa
    13261 ccggtgcctg ttatcactga aagcatcatt cagacactgg gtgatgaatt gctacaaaaa
    13321 gctgagaaga aaaaaaacat tgtctggcgt tatgaaaatt tttcactgga gtggcagtcc
    13381 gccatcacgc aggccatcaa cttgatcggc gaacacaaac cctcaatccc ggcccggaca
    13441 atggcggcgc tagcctgtat cgcgcaaaat gacagccaac agttgctcga cgaaatcgtc
    13501 caacaagagg ggctggaata tgcgactgag gtggtgattg cacgccagtt tattgcgcgg
    13561 tgttatgaga gtgatcctct ggtagtgaca ttgcagtatc aggacgagga ttatggctat
    13621 ggttatcgct cagaaaccta taacgaattc gatctccgac tgcgtaagca tctctctctg
    13681 gcagaggaaa gctgctggca gcgttgcgcc gacaaactca ttgccgcact accaggaata
    13741 accaaagttc gccgcccttt tattgcgctg atcctcccgg aaaaaccaga aatagccaat
    13801 gagttggtag gccttgaatg cccgcgaact cattttcatt ctaaggagtg gttaaaagtt
    13861 gttgctaatg accccacagc ggtgagaaaa ctcgagcact actggagcca ggatatattt
    13921 agcgatcgag aagccagcta catgtcgcat gaaaaccact tcggctacgc ggcctgcgcc
    13981 gcccttttgc gcgaacaagg actggcagcc attccgcgcc tcgcgatgta tgcccataaa
    14041 gaagattgcg gcagtctgct ggtacaaatt aaccatccgc aagtcatccg caccttgcta
    14101 ctggtggctg ataaaaacaa acccagcctg caacgtgtag ctaaatacca taaaaacttc
    14161 ccccatgcga cgctcgccgc actggcagaa ctgctggcgt taacagaacc accagcccgc
    14221 ccaggttatc caatcatcga agacaaaaag ctgcctgcac agcaaaaagc acgcgatgaa
    14281 tactggcgta cgctgttaca aacgctgatg gcatcgcagc cacaactggc agaagagatg
    14341 atgcagtggt taagtactca agccagggca gtgctgaata gttatttatc ggcaccgccc
    14401 aaaccggtta ttgatagtac cgataacagc aatctgcctg aaatcctcgt ttcaccaccg
    14461 tggcgtagta agaaaaaaat gacagctcca cgtcttgatt tggcaccgct cgaattaacg
    14521 ccgcaagttt actggcaacc tggcgaacga gagaggcttg ccgccactga gcctgcccgt
    14581 tatttcagca cggaatctct tgcggaacgc atggaacaaa aaagtgggcg agttgtatta
    14641 caggaactgg gttttgggga tgatgtatgg ctgtttttga attatatact ccccggaaaa
    14701 ctggatgctg cacgcaattc actcattgtt cagtggcatt actaccaggg gcgggttgaa
    14761 gagatcctga atggctggaa ctccccggaa gcacaattag cagaacaggc gctccgcagc
    14821 ggtcacatag aagcgttaat taacatatgg gaaaatgaca actactcacg ttatcgtccg
    14881 gaaaagagtg tctggaacct gtatttattg gcacagttgc cgcgtgagat ggctttgacc
    14941 ttctggctgc gtatcaatga gaaaaaacat ctgttcgcgg gtgaggacta ttttctcagt
    15001 atcctcgggt tggatgcgct accaggtctg atgttggctt tttcacatcg tccaaaagaa
    15061 acatttccgt taattttaaa tttcggcgca acagaactgg ccctgcccat tgcccgcgtc
    15121 tggcaccgtt ttgcgggcca gcgtaatctg gctcgccagt ggattttaca atggccggaa
    15181 catacggcta ctgcacttat ccctcttatc ttcaccaaat ctagcgataa aagcgaagct
    15241 gcattacttg ccctgcgttt actttacgaa catgggcatg gcgaattgct acaaactgta
    15301 gccaatcgtt ggcagcgtac agatctatgg cctgccctgg aacaattact taaacagagt
    15361 ccaatagaga tttacccggc acgcattcca aaagcccctg atttctggca tccgcaaatg
    15421 tggtccaggc cgcgccttat cactaataat caacctgtta ccgatgacgc tctggaaatt
    15481 atcggcgaaa tgctgcgctt tacccagggg ggacgttttt atagcgggct ggaacaactg
    15541 aaaacgttct gccagccaca aacgctggca gcttttgcct gggatctctt cactgcgtgg
    15601 caacaagctg gtgcccccgt aaaagacaac tgggcatttc tggcgttaag tctctttggt
    15661 gacgaaagca cggcacggga tctaacgaca cagatcctcg gctggccaca agaaggcaaa
    15721 tctgcccgtg ctgtcagtgg cctgaacatc cttaccctga tgaataatga tatggcgctg
    15781 atacagctgc atcatatatc gcaacgggcg aaatcccgcc ccttacgtga taacgcggcg
    15841 gaatttcttc aggtggtcgc agaaaatcgc gggctaagcc aggaagagct agcggacaga
    15901 ttagtcccaa ccctgggcct tgatgatccg caggcgttga gttttgattt tggtccccgg
    15961 cagtttaccg ttcgcttcga tgaaaacctc aacccggtta tctttgatca gcaaaacgtt
    16021 cgccagaaaa gcgttccccg gttgcgcgcc gatgacgatc aactgaaagc gcccgaggca
    16081 ctggcccgac taaaagggct aaaaaaagat gccactcagg tgagcaaaaa cctgctcccg
    16141 cgtcttgaaa cggccctacg taccacccga cgctggtcgc tggcagattt tcattctctg
    16201 tttgttaatc atccctttac ccgtctggtt acccagcgat taatatgggg ggcttatccg
    16261 gcaaatgaac cgcgttgttt actcaacgcc tttcgtgtgg ccgcagaggg ggagttctgc
    16321 aatgcgcaag atgagccaat tgacctgcct gcggacgctc tgattggcat tgcccacccg
    16381 ttagaaatga cagtagaaat gcgcagtgaa tttgcacagc tttttgccga ttacgaaatt
    16441 atgccgcctt ttcgccagtt ggcgcggtgc acggtgctgc tcacacctga tgagtcaacc
    16501 agtaacagcc tgactcgctg ggaaggtaaa tccgctaccg ttgggcagct tatgggaatg
    16561 cgatacaaag gctgggagtc aggctatgag gatgcatttg tctatgacct gggcgagtac
    16621 cggctggtcc ttaagttttc acccggtttt aaccactaca atgttgatag caaagcgcta
    16681 atgagcttcc gttctcttcg agtgtaccgt gacaataaat ccgtcacttt tgccgaactt
    16741 gatgtgtttg atttgagtga ggcgttaagc gcacctgacg tcattttcca ttaaaacaca
    16801 gcgggcagtg atgcgactgc ccgttatcta cacgacttac cagcggggaa agcgatgatc
    16861 gtgcaaaaag agctagttgc tatttacgat tatgaggtcc ctgtacctga agatccgttt
    16921 tccttcagac ttgagatcca taaatgctct gaattattta caggttccgt ctatcgactg
    16981 gagcgattcc ggctacatcc aacatttcat caacgtgatc gagaagatgc tgacccgcta
    17041 ataaatgatg cgttgattta tataagagat gagtgtattg atgagcggaa attacgaggt
    17101 gaatcacctg aaactgtaat agcaattttt aatcgtgaac tacagaatat attcaaccaa
    17161 gaaatagaat aatatactct agataattaa agtcgcagca catgcaactt gaagtatgac
    17221 gagtatagct ttcttaaagg gggaaacggt attccccctt agatgcgttt caatttgccc
    17281 gttacttcaa caaatcaatc aaccgtaaca gagccactaa taactccagt acggcaatga
    17341 gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc ttattaagga gcgcaacacc
    17401 agcaccttac ccttacactg cctgttgata ctggtttacg tatgttggta cgaaacgttg
    17461 ctctccggat cagggcaccg cctgcggcac cacccgtccg gcagccagga cttaaaattg
    17521 gtccgatccg gcacaacctc ccaacgggca ggattataaa tctcactcac tgaattgcaa
    17581 tggagtgtgg atatggttat aatttattcg ttggtacgaa atgttgcaca atcagcaagt
    17641 tagcgtcact gtagtaaata aaagaacctg cctcaccagc aggttttttt attttctgcg
    17701 atctactttc cagatatttt ttgccaatag cccaacataa atattcacat cccggtcaaa
    17761 tggtgcgttg aatataaaat cgatgaattt ataaataaaa atgagttata tgatgaatta
    17821 ataaaaacag taatcatttt attaaagggg gaagcggttt tcccccttag atgcgtttca
    17881 atttgcccgt tacttcaaca aatcaatcaa ccgtaacaga gccactaata gctccagtac
    17941 agcaatgagc acggttaact tttgtgtcag cgtgtccatc cgctctcctt attaaggagc
    18001 gcaacaccag caccttaccc ttacactgcc tgtcgatact ggtttacgta tgttggacca
    18061 cgatgttgca ctccggatca gggcaccacc tgcagcaccc cccgtccggt agccaggact
    18121 tgaaataggt tcgatccggc gcaacgtccc aacggtctgg attataaatc tcattatctt
    18181 aattgcaacg gggtccagcc gtggttataa ttcgttcgtt ggaacacgat gttgcacagg
    18241 ctgtggtgtt aggcctgaaa atagtaaata aaagaacctg cctcaccagc aggttttttt
    18301 atttactgcg atctgctttc cagatatttt ttgctcaaac aactaatgcg ccaaacattt
    18361 attgcgcgta aaatatcgtt tatttcatta atacatttca gggatgaata tatgtcaccg
    18421 cagaataatc atctgcagcg tccgcctgct gctgtgttat acgccgatga actggcaaaa
    18481 ttaaaacaaa atgataacgc tccttgcccg cccggttggc agttaagttt gcctgcggcc
    18541 cgtgctttta tccttggcga tagcgcgcaa aatattagcc gtaaagtggt gattagtccc
    18601 tccgctgtcg aacgtatgtt agtcactctt gccaccggac gcggtttgat gttggtgggg
    18661 gaacctggca ccgcaaaatc tctcctgtct gaattactgg caaccgctat tagcggcgat
    18721 gccgggttaa ccatccaggg cggcgcatct accactgaag atcaaatcaa gtacggctgg
    18781 aactacgctc tgcttatcaa tcatggccca tcaacggaag cgctggtccc tgccccgctt
    18841 taccagggaa tgcgcgacgg caaaatcgtc cgttttgaag agataacgcg tacaccgctg
    18901 gaagtgcaag actgtctgtt aggtatgctt tctgacaggg tgatgacggt gccggaactc
    18961 actggtgaag ccagtcagct ctatgcgcga gaaggcttta atattattgc cactgccaat
    19021 acccgagatc gcggcgtcaa tgaaatgagt gctgcgctaa agcgtcgctt cgattttgaa
    19081 actgtctttc cgattatgga tttcgcccag gagctggagc ttgtcgccag tgcttcggcg
    19141 cgtttgctgg cccatagcgg tattccgcat aaagtgcctg acgcggtact ggagttactg
    19201 gtccgcacct tccgcgatct gcgtgccaac ggcgaaaaga aaacgtcaat ggatacacta
    19261 acagcgatta tgtccaccgc tgaagccgtg aacgtggccc acgccgtagg cgtccgcgcc
    19321 tggtttttag cgaatcgcgc aggtgagcca gccgacctgg tggactgcat tgccggaacc
    19381 atcgtcaaag ataacgaaga agatcgcgct cgtctgcgcc gttacttcga acaacgagtc
    19441 gccacacata aagaagctca ctggcaggct tattatcaag cccgccaccg cctgccgtga
    19501 ggaaagatgc atgagcgagc cgttaattgt cggcatccgg catcatagtc cggcctgcgc
    19561 ccggctggtg aaatcgttaa tcgaaagcca gcggccacga tacgtattga ttgaaggccc
    19621 ggctgatttt aatgaccggg tagacgaact gtttttagcc caccagcttc cggtagctat
    19681 ttacagttat tgccagtatc aggacggtgc agcccccggg cgtggtgcct ggacgccatt
    19741 tgctgaattt tcgccggagt ggcaggcgct acaagccgca cgtcgtattc aggcacaaac
    19801 ttacttcatc gatttgcctt gctgggcgca gagtgaagaa gtggacgatt cgcctgatac
    19861 gcaagaggaa agccaggcct tgctgctgcg tgccacccgc atggataaca gcgacaccct
    19921 gtgggatcac ttgttcgaag atgaaagcca gcaaactgca ttaccctctg cactggcgca
    19981 ctattttgcc caactgcggg gcgactcccc cggagatgct ctcaatcgtc tgcgcgaagc
    20041 ctttatggcc cgctggattg gatgggcgat gcagcaaaat aatggcgacg tgttagttgt
    20101 ctgcggtggc tggcacgctc cggtactggc aaaaatgtgg cgcgaatgcc cgcaggaaat
    20161 taacacgcca gaattgccgt cgctggcaga tgccgttaca ggttgttatc tcacccccta
    20221 cagtgaaaag cgccttgatg tgctggcagg atacctttcc ggaatgcctg ccccggtctg
    20281 gcaaaactgg tgctggcagt ggggcttaca gcaggcggga gaacaactgc taaaaacggt
    20341 tctcacccgt ttgcgccagc acaagttgcc tgcttcgacc gcggatatgg ctgccgctca
    20401 tctgcatgcg atggcactgg cacagttgcg cggtcataca ctaccgttac gcactgactg
    20461 gctggatgcc atagcaggat cgctgattaa agaagccctg aatgcgccgt tgccgtggag
    20521 ctatcgcggc gttattcatc ccgacaccga tccgattctg ctaacgttga tagacacatt
    20581 agcgggtgac ggattcggta aacttgcccc ttctacgcca caacctcctc tgccaaaaga
    20641 tgtcacctgc gaactggaac gtaccgcaat ctcccttccg gcggagctta ccttaaatcg
    20701 ctttaacccc aatggactag cgcaaagtca ggtgttacat cggctggcaa tactggagat
    20761 cccagggatt gtgcgccagc agggaagcac actgacactt gcaggcaacg gtgaagaacg
    20821 ctggaaatta acccgcccgc ttagccagca tgcggcattg attgaggccg cctgctttgg
    20881 tgcaacactc caggaagccg cacgccataa attagaagcc gatatgctgg acgcgggtgg
    20941 aatcggcagt atcaccacat gtcttagcca ggcggcgtta gcgggtctgg cgtccttcag
    21001 tcaacaatta ctggagcaac tcacattatt aatcgcccag gaaaatcaat ttgccgaaat
    21061 gggccaggcg ctggaagtgc tatatgcctt atggcggctg gatgaaatta gcggtatgca
    21121 aggcgcgcag atattacaaa cgacgttatg cgcggctatc gatcgcacgc tgtggctatg
    21181 tgaatctaac ggcagaccag atgaaaagga gtttcacgct cacctgcata gctggcaagc
    21241 gttgtgtcat atcctgcgcg atctacatag cggtgtgcaa ctccccggca tttctctttc
    21301 tgcggcggta gccttactgg agcgacgcag tcaggcaatt catgccccgg cgctggatcg
    21361 cggcgcggct catggcgcac tgatgcgtct ggaacatcct aacgccagtg ccgaagcggc
    21421 gctgacgatg ctggcgcagt tatccccggc acaatccggc gaagcgctgc acggtttgct
    21481 ggcgctggcc cgccatcaac tggcctgtca gccgacattt atcgccggtt tcagcagtca
    21541 tttaaatcaa ctaagtgatg ccgattttat caatgccctg cccgatttac gcgcggcgat
    21601 ggcctggcta ccaccacgag aacgcgggac gctggcgcat caggtgcttg agcattatca
    21661 actggcgcaa cttcccgttt cggcgctgca aatgccgttg cattgtccac cacaggccat
    21721 tgcacatcat caacaactcg aacagcaggc actggcatcg ctgcaaaact ggggagtttt
    21781 ccatgtctga actgaacgat cttctgacca cccgtgagct acaacgctgg cgattaattc
    21841 ttggcgaagc ggcagaaacg acgctttgtg ggctggatga caacgcccgg cagatagacc
    21901 acgcgctgga gtggctgtat gggcgcgatc ctgaacggct ccagcgtggt gaacgctctg
    21961 gtggattagg tggctcaaat ctcaccaccc ctgagtggat caacagtatt cacactctgt
    22021 ttccgcaaca ggtgattgag cgactggaaa gcgatgccgt actgcgctac ggcattgaag
    22081 atgtggtgac gaatctcgac gtgctggaac gtatgcagcc ttcggaaagc ctgctacgcg
    22141 ccgttttgca caccaaacat ctgatgaatc ccgaagtact ggctgccgcc cgccagatag
    22201 tgcgccaggt tgttgaagaa attatggcgc gactggcaaa ggaagttcgt caggcttttt
    22261 ctggtgtccg cgatcgccgt cgccgctcat taattccact ggcgcgaaac tttgatttca
    22321 aaagtactct gcgcgccaac ctgcaacact ggcacccgca acacggcaag ttgtatatcg
    22381 aatccccccg ctttaacagc cgcattaaac gccaaagcga acaatggcaa ctggtcttac
    22441 tggttgatca aagcggatcg atggtggact cggtgatcca ctctgcggtg atggcggcct
    22501 gtttgtggca gttacccggc attcgtaccc atctggtggc gtttgacaca agcgtggttg
    22561 atctcacggc agacgttgcc gatccggtag agttattaat gaaagtacag ttgggcggcg
    22621 ggaccaatat cgccagtgcc gtggagtatg gtcggcaact tattgaacaa ccagcaaaaa
    22681 gcgtcattat cctcgtgagc gatttttacg aagggggttc atcatcattg ctgacgcatc
    22741 aggtgaaaaa gtgtgtccag agcggcatca aagtgctggg actggcagcg ctcgatagca
    22801 ccgcaacgcc ttgctatgac cgcgatacgg cccaggcgct ggttaatgtc ggcgcacaaa
    22861 tagccgccat gacgccgggc gagctggcat catggcttgc ggagaatctt cagtcatgaa
    22921 ttcactacgt ccggaattat tagaactgac accgcaggca ctgacggcgt taagcaatgc
    22981 cggttttgtt aagcgcagtc ttaaggaact ggaaaatggc aacgtcccgg aaatcagcca
    23041 tgagaacggc gctttaatcg ccaccttcag tgacggtgtc cgtacccagc tggcgaacag
    23101 ccaggcactg aaagaggctc agtgcagctg cggggccagc ggcatgtgcc gtcatcgcgt
    23161 gatgctggtg ttaagttatc aacgactttg tgccaccgtc cagcccacgg aaaaagaaga
    23221 agagtgggat ccggcaatct ggctggaaga actggctacc ctgcccgatg ccacccgcaa
    23281 acgcgcgcag gcgctggtcg ctaaaggcat caccattgag ttgttctgtg cgccgggcga
    23341 aattccctct gcccgcttac cgatgagcga tgtgcgtttt tattcccgta gcagtattcg
    23401 tttcgcccgt tgtgattgta ttgaaggcac actttgcgaa catgtcgtac tggctgtgca
    23461 ggccttcgtc gaggccaaag cgcagcaagc agaatttaac catttaatct ggcagatgcg
    23521 cagcgaacac gtcacatcat ctgacgatcc gtttgccagc gaagaaggcc aaacgtgtcg
    23581 tcaatatgtt cagcaattaa gccaggcatt atggcttggc ggcatcagcc agccgcttat
    23641 tcactacgag gcagcattca accgcgcatt gcaggcggca gaggcctgca actggcgctg
    23701 ggtgagtgaa tcactacgac agctacgggc aagcgttgat gccttccaca ctcgcgccag
    23761 ccactatcat gccggagaat gcttacgtca gcttgcggca ttaaacagtc gattaaattg
    23821 cgcacaagag atggcccggc gcgacagtgt tggtgaagtt cctcctgtgc cgtggcgcac
    23881 ggtcgttggc tctggcattg ccggagaagc aaagcttgat catctgcggc tggtgtcttt
    23941 aggtatgcgt tgctggcagg atattgagca ttatggttta cgcatctggt ttaccgatcc
    24001 cgacaccggc agtattttgc acctttcgcg cagttggccg cgaagtgaac aggaaaactc
    24061 accggcagct acgcgtcggc tgtttagttt tcaggctggc gcactggcgg gtgggcaaat
    24121 tgtttcacaa gcagcaaaac gcagtgccga tggcgagctg ctgttagcta cccgcaaccg
    24181 cttaagcagc gttgtgccgc tgtcgcctga tgcctggcaa atgttgagcg cgccgttacg
    24241 ccagccgggc attgtggctt tgcgggaata tttacgccag cgtccccccg cctgcatacg
    24301 gcctcttaat caggtcgata acttatttat tctgccggtc gctgagtgta tttcgctcgg
    24361 ttgggatagc agccgccaga cgctggatgc gcaggtaatc agcggcgaag gtgaagataa
    24421 tctcctgacg ttatcccttc cggcatctgc ctgttctcct tttgccgttg aacgcatggc
    24481 ggcgcttttg caacaaacag acgaccccgt gagtctggtt tctggctttg tcagttttgt
    24541 tgatgggcaa ttgacactgg aaccacgggt gatgatgaca aaaacccgcg cctgggcgct
    24601 ggacgcggaa actgcgcctg tggcaccgct accttctgcc agcgttttgc ctgtgccgtc
    24661 taccgctcat cagttgctga tgcgctgcca ggcgttactt attcaactgc tccataacgg
    24721 ctggcgctat caggaacaga gcgctattag tcaggcagag ttgctggcga atgacctctc
    24781 cgcggtcggt ttttatcggc tggcacatgt gttgggacaa tttcgtaata cagaaagcga
    24841 ggcacgggta gaagcaatga ataacggtgt tttactttgc gaacaattat tccccatgct
    24901 tcagcaacaa ggatgaaata gtgcttttta ctaagagttc tactccagtt ccggactgct
    24961 cacgtcacgg tattaggcat attctatata gcccctggtg agagtcacca gtttcttgat
    25021 taaataaaat ggagttttac atgaaggctt tcaataagct gttttccctc gttgttgcat
    25081 ctgttctggt tttctctctt gctggctgcg gtgacaaaga agaatcgaag aaattcagcg
    25141 ccaatctgaa cggcactgaa attgccatta cctatgtcta caaaggtgac aaggtgctta
    25201 agcaatcttc tgaaaccaaa attcaatttg cttctattgg tgcaaccacc aaagaagacg
    25261 ctgccaagac acttgagccg ttaagcgcca aatacaaaaa catcgcgggt gttgaagaaa
    25321 aattaaccta tacagatacc tacgcgcagg aaaacgtgac tatcgatatg gaaaaagtgg
    25381 attttaaagc cctgcagggt atttcaggaa tcaacgtttc tgctgaagat gccaaaaaag
    25441 gtatcactat ggcgcaaatg gaactggtga tgaaagccgc tggttttaaa gaagtgaaat
    25501 aatctgtcgg cggccatgtt tcgcatggcc gccatacccg tcttagcttt tcttcacatg
    25561 ctggcgcgct gccagtccac gcagaaaata acgtaaaaat tgatcgccgc attcgcggaa
    25621 gtttttatga tccggtgcgc gcatcatcgc tgtaatttcc ggcatcgaaa cgcggaactg
    25681 ctgttcggtg aggatagcca gaatgtcatc ggttttcagc gaaaacgcga tgcgtaattt
    25741 tttcagcacg atgttgttat taatgcgacg ttccggctcc agtgccggag cagactcatc
    25801 cttgccgcgt ttttcataaa tcaggccgtt gaggaatgac gacaaaacaa tgtccggaca
    25861 acgctgaaaa ccctcttcgt cttctttacg tagccagacg gcgatctgtt ccgcggtggc
    25921 ttcgacatta cccagcgcca gaatacgcac caggtcatta ttattggctt tcaaaatgta
    25981 gcgcacgctg cgcagaatat cgttacttag catgaggcct tcaggtgttg atgaggcaaa
    26041 aagccatttt agcagtcttt tacaggccaa tcgcctcttt taagcttttc agataacggc
    26101 ggctgaccgg cacggttaag ccattacgca aaatcaactc ggcctggccg ttatcttcca
    26161 gacgaatctc ctgtaaatgc gcgagattaa ccagatactg acgatggcag cgcagtagtg
    26221 gtgtacgact ttccagggta cgtaatgtca attcggtaaa gccctctttc ccttcgtggc
    26281 tggtaacgta gacaccgctc atccgactgc tgacaaatgc cacatctttc atttgcagca
    26341 aataaatccg actatgcccc gtacaaggga taaatttcag cgcctgttga ttttccggta
    26401 acagcgaaac atcctgcttg ctgcgctcct gacgcaatcg cgccagcgtt ttctccagtc
    26461 gcgcttcatc aattggcttc agcagataat caaaagcatg ttcttcaaag gctttaatag
    26521 cgtattcgtc aaacgcggtg agaaaaacaa tatacggacg atgttccgga tcgagcatcc
    26581 ccaccatttc cagaccactg atgcgcggca tctggatatc gagaaacagc acatccgggc
    26641 gcagtttatg caccgcgccg atcccttcca cggcgtttga acactctcca acgatttcaa
    26701 tatcgctctg ctcctgcaaa aatacgcgca ggttctcccg tgctaacggt tcatcatcga
    26761 caattaagac tttaatcatg cctcgtccct ccatggtagt cgtaacgtta ttcgggtgta
    26821 actatcaggc tcacaggcga cgcttatccc atagtcatcg ccaaaccgtt cacgtaaacg
    26881 cttatccacc agattcatcc ccagcccact ggcattggtt accggttgat acaaaccggc
    26941 attgtcttcg atctccagca tcaaatgttg cccctcacgt cgggcgctga ttgccactcg
    27001 ccctgtatcc agcagttgtg atgtcccatg tttaatggcg ttttccacta tcggttgcag
    27061 ggtaaacgcg ggcaattgct gctgggataa ttcttgcgga atagcaatgt tgacctgcaa
    27121 ccgcgactgg aagcgcgcct tttcaatttg cagataagcg ttcacatgtt caatttcgtc
    27181 ggcgagagta acaaactccg aaggccgctt taagtttttg cggaaaaaag tggaaagata
    27241 ctgcaccagc tggctggcct gttcgctgtc gcggcggatc accgctttaa tggtgttaag
    27301 cgcattaaac aaaaaatgag gattcacctg ggcgtgaagc agtttgattt ctgactgggt
    27361 gagcatcgct ttttgccgct catattgccc ggcaagaatc tgcgccgaaa gcagttgcgc
    27421 aatcccctcg cccagcgtgc ggtttattga actgaataaa cggtttttgg cttcatacaa
    27481 tttgatggtg cccatcacgc gctgattttc gccacgcaac ggaattacca gcgtcgaccc
    27541 cagtttgcat tgcggatgca aagagcaacg ataaggtact tcgttgccat cagcgtagac
    27601 cacttcaccg gtttcaatcg cttttaaggt gtaagtcgaa gaaatcggtt tgccgggtaa
    27661 atggtggtcg tcaccaattc cggtaaaggc cagcaatttc tctcgatcgg taatcgcgac
    27721 tgcaccaata tccagctcct gatacagcac ctgagccact ttcatgctgt tcacttcgtt
    27781 aaacccctgt cgcaaaatgc cttccgtcga ggctgccact ttcagcgcag tggcagaaaa
    27841 agcagaagtg tatttttcaa acatcgcgcg tttatcgagc aatatacgca taaacagcgc
    27901 cgcgccgacg gtattggtga ccatcattgg tgcagcaata ttactcacca gacgcaccgc
    27961 atcttcataa ggtcgggcga tcgcaaggat gatcagcatt tgcaccattt cagcgacgaa
    28021 cgtgacggca ccggcggtaa tggggttaaa gactttatca gtgcgcccgc gacggatcag
    28081 gatgctgtgt accaggccgc cgagcaatcc ttcaacgatg gttgagatca tgcaacttag
    28141 cgcggtcatg ccccccatcg aatatcgatg taagccgccg gtcagaccaa ccagcccacc
    28201 gacgaccgga ccgccgagta agccgcccat taccgcgcct atcgcacggg tattggcaat
    28261 agaatcgtca atgtgcaacc caaaccaggt gcccatgatg cagaagatgg aaaagacgat
    28321 gtagcagaga aatttatgcg gcagacgaac cgtgacctgc attaacggta tgaataatgg
    28381 cgttttactc attaaccacg caatgactaa aaaaacgcac atctgctgaa gcagcagcaa
    28441 caccagatta aaatcgtaca tacccgcaaa ccacacttcc ctttaaaacg cgtaacatac
    28501 attgcctgcg tttaactttc tttgaactct tgcagaaaaa tgagaattcg tgagtacgat
    28561 cactcaaaat cgcctggcaa aaataaaatc accctataga tgcacaaaaa acgggcaaaa
    28621 ctacctggtt cgcaaaactg cgtctaaagt taaaccggga cctcgcgagc aagggtgaga
    28681 cgatggcgct ttacacaatt ggtgaagtgg cgttgctttg tgatattaac cctgtcacgt
    28741 tacgcgcgtg gcagaggcgt tacgggttgc tgaaaccgca acggacagac ggcggtcatc
    28801 gactgttcaa cgatgccgat attgaccgta tccgcgagat caaacgctgg atcgacaacg
    28861 gcgtgcaggt cagcaaagtt aaaatgctgc tcagtaatga aaatgttgat gtgcagaacg
    28921 gctggcgcga tcagcaagaa acattactga cctacctgca aagcggcaat ctgcatagcc
    28981 tgcgaacgtg gatcaaagag cgcggtcagg attaccccgc ccagacactc accacacatc
    29041 tgtttattcc tctgcgccga cggcttcagt gccaacaacc gactctccag gcgctgctgg
    29101 cgatcctcga cggcgtactg atcaactaca tcgccatttg tctggcttcg gcacgtaaaa
    29161 aacagggtaa agatgcgctg gtggttggct ggaatattca ggataccacc cgtctgtggc
    29221 tggagggctg gattgccagt caacaaggat ggcgcattga tgtcctcgcc cactcgctca
    29281 atcaactacg ccctgaacta ttcgaaggcc gtacattgct ggtgtggtgc ggtgaaaatc
    29341 gaacctccgc ccaacagcag caactcacca gttggcaaga acaaggccat gatattttcc
    29401 cactcggcat ttaatgattc gttaacaaat gcgctttact gtacaatcct ttcgttaaca
    29461 taaggagtgc aatatgcgca tagctaaaat tggggtcatc gccctgttcc tgtttatggc
    29521 gttaggcgga attggtggcg tcatgctcgc aggttatacc tttattttgc gtgctggcta
    29581 agcgcctgca ccagcctttc aaacaggcgg tctgcgatga tcgccgccag tgccaccagt
    29641 aacgccccct ggatcacata cgcggtatta aatccgctaa gcccgatgat gatgggcgta
    29701 cccagcgtgc tggcccctac cgttgaggcg atcgtcgccg taccaatgtt gataatcacc
    29761 gaagttcgca cgcccgccag aatcaccgga gccgccagcg gtagctcgac cttacgcagt
    29821 cgctgaccac gactcattcc catacctttc gcaacttctg tcacgctggc atcaatcgct
    29881 cccagcccgg caagtgtcgc ctgcaggacg ggcagcacac cgtaaaggat caaggcgata
    29941 atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa tcgccagcac tgcgacaggc
    30001 ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg ggcgaaattc cgcgccccac
    30061 gggcgagtga cagcaattcc ggcaccagtg ccaatgatca ccgcaaacaa actcgaaatt
    30121 cccaccagcc agaaatgagc cagtgccaga gctgcaaaac tttcttgctg ataaacgggt
    30181 cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc tgtaaggcag ccagaaaatc
    30241 agtgccacaa acaaagcaat gagccagaac agcggatcgc gcaacatttt catacgctta
    30301 cgcctcctcc agcagatcct gaaaatgcag cgtgccgcaa gactggccct gcgtgttcat
    30361 caccggcagc acctcgcatc cccgcgcgac aaacagggag agcgcatcgc gtagcgtcat
    30421 ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga cgcacgtaat ccgccacact
    30481 acgtaacgaa agcaggcgca cccccagttc actacgtcca aaaaactggc ggacaaaatc
    30541 attcgccgga cgagtcagca tcgtcagcgg attcccctgc tgcaccactt caccgtgatc
    30601 catcaatacc agatgttctg ccagccttag cgcctcatca atatcatgag tgaccagcac
    30661 aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct tgttgcaacg cgccgcgcgt
    30721 taccgggtcc agtgcgccaa aaggttcatc catcagtaag acttgcggat cggcagccag
    30781 tgcgcgcgcc actcccacac gttgctgctg accaccggaa agctgatgcg gataacgctc
    30841 acgcaaattt gactccagcc ccagtagcgt cattaattcg tcgatacgat cgtcaatccg
    30901 tgcccgcgac catttttgta attgcggcac ggtagcgatg ttttgcgcca cgctccagtg
    30961 ggggaacagg ccaatagatt gaatggcata gcccatccgg cggcgcaact ccagtactgg
    31021 cagcgagcga atttcttctc cggcaaagcg gatctctccg ctgtcatgtt ccaccaggcg
    31081 gttaatcatt ttcaaggtgg tggatttgcc ggagccagat gtgccaatca gcaccgaaaa
    31141 actcccttcc tgaaaattga gattaagatc gttaacggct ttttgtgcgc caaacagttt
    31201 gctgacatgg ctaaattcaa tcattacgtt tcaccttcag cagtgcgata agcaaatcga
    31261 acagggcatc gatcagcacc gccagaacaa ttaccgggat cacccccagc aacactaaat
    31321 caatggcgct gcttagcagc ccctggaaaa ccagcgcacc aaaaccgcct gcgccgatta
    31381 acgccgcaat caccgccata cctacagttt gcaccatcac cacccgcagg ctgcgcagaa
    31441 ataccggtaa cgccagcggt aactgaacat gcaggaatcg ctgcgccccg ctcatcccca
    31501 tcgctctggc gctctccagc acatcgcgcg ggatctggtt caagcctact accacgccgc
    31561 gcaccagcgg cagcaaggca tagagcacca gcgcaatcag tgcgggtgtc attccggttc
    31621 ctgctatgcc gagcttcccc agccacggaa aggccgtcac cagcgcggca agcggcgcaa
    31681 tcaacaggcc aaagagcgcc accgaaggca cggtctgaat gacattgagc agagaaaaaa
    31741 ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc caacggcaca ccaatcacta
    31801 acgcaggcag caccgcacca aacagcaacg tcagatgttg tgccagcgcg tcgtcaaaca
    31861 catcctgacg gttggcgtat tctttcatta gcgagagatc gttaagcgtg ccggagtaca
    31921 gcaaccacag cggaataatg gcaatctgca tatgcaacaa ccagcgccac agcggatgcg
    31981 tggagattcg gcggatggca tcgctacagg ccagcaatgc cagcgccgca gccagccaga
    32041 aaccactgcc gaggctggta cgcgccagcg cactgccatt ttgcgccagt tgggtcgccg
    32101 cttttccagc tccccacacc agcaatacga agacgaattg cgccagaatg agtgcaaaaa
    32161 tgctgccttt tttagcggga ataaaacagg ctgtcagcca ggcgcaacca aagcccacca
    32221 gcatccagat cgtttgcggc cacagctgcc agagatgtcg cccttcaccc gagactaaac
    32281 gattaggcgc gtagctgata aacggcagcg ctgctgcgat tgccgtcagc aacagcagca
    32341 gcgccagaac aggattaata cgtagataag tcacgggaaa ttacttcgtc cacccttttt
    32401 gtttcaggta gtcggcagcc acttttttgg catccagtcc ttccactgca atgctggcat
    32461 tcagttgctg caatgttttt gcatcgaggc tggcgaagac tggctgtagc cactgtgcca
    32521 tttgcggata ctctttcagc accgactcac gcaccactgg tgcaggcgcg tagataggtt
    32581 gcacgccttg cggatcgctt aaggtttgca gccccagcgc cgctaccggg ccgtcagtgc
    32641 cgtaagccat tgcagcatta acgccagagg tttgctgggc agcggctttg atcgtcaccg
    32701 ccgtgtcgcc accagccagt gacagcaact gatcctgacc gagcttaaag ccataggctt
    32761 tttcaaacgc gggtaacgca tcggcgcgtt cgataaactc tgccgaggct gccagtttga
    32821 aggtgccgcc ctcttgcaga taacgactca ggtcggcaag cgaagtgagt ttatttttct
    32881 ctgccacatc ctgacgcacg gcgatagtcc aggtgttatt tgcaggcgcg ggcgtcagcc
    32941 agattaactt gttgtgctcc gaatcgagct ttttgacttt ctcgtaacct tgctgcgcgt
    33001 ttttccacgc tgcatcgttt tcatctttaa agaagaaagc gccattgccg gtatattccg
    33061 gatagatatc cagttcaccg gaagtaatcg ccccgcgcac cacaggagtc gttccgagtt
    33121 gcactttatt caccgtcggt acgccgtggc tttccagcac ctgcaaaatg atattgccga
    33181 gtagcgcacc ttcggtatcg atttttgaac cgactttaac gggggaagcc gcctgtagcg
    33241 gcaggctcac ggctgccaac ataaccagtg aacctgccca gagctttaag agtggcatga
    33301 tgctttcctc attcttttac tgttgttttc agcgaattaa gagaaaagca tagttgataa
    33361 tggcggggtt agcttgagcg gaaggattca gttgcagaat cagataaata cttaagaggc
    33421 atattcggtc cggcgtttcc tgccggatgc ggcgcgagcg ccttatccgg cctacaaagg
    33481 gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc aaactcgcct ttcttaacgc
    33541 gtgcggaatc agtgccgata aatacattga acttgccagg ctcggcgtca tatttcatct
    33601 gttgattcca gaacttcagc gcctcaatat cgatcgggaa gctgacagtc tgagtttcgc
    33661 ccggtttcag ggtgattttc tcaaagcctt tcagctgttt cacagggcga ctcatggaag
    33721 ccgtcacatc ctgcaagtac atctgcacta ccgtggcccc ctcgcgctta ccggtgttcg
    33781 tcacctgcac gctggcagtc actttgccgt cacgcttcat ggtcggcgca gaaagtttca
    33841 catcagagac ggtgaaagtg gtgtagctca gcccatagcc gaacggatac aacgccccgt
    33901 tagcttcatc aaaataacgc gaagtgtatt tgttcggctt gtcggcatta tacgggcgac
    33961 cggtattcag atggctgtag tacaccggga tctgcccgac agaacgcggg aaggacattg
    34021 gcagcttgcc ggacgggttg taatcgccaa acaatacatc ggcaattgca ttaccgcctt
    34081 cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgatcttct ttcaccagcg
    34141 ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct ttcagcgccg
    34201 caatcaagtc acgttggctt tgcggaatag tgatatcggt ccggctggag gcttcgtgcg
    34261 ccatcccctg tgcttcaccg actacagcca ccaccacatc agattgtttc gccgtctgca
    34321 ccgcttcatc aatcatctct tgcggcgagc gcggatcgac tttgaccgct tcttcatact
    34381 gattcaggaa atcgataatg cctttgtcac tggtaacgtt cgcccctttg gcatacagca
    34441 ctttaccgtt ttcaccgacg gcatttttga tcccggtcag tacggtcacg gattgatcgg
    34501 caacaccggc tgcggaccag ctgcccatca cgtcacgttt actgtccgcc agtggcccaa
    34561 ccaccgcaat ggtggccgat tttttcagcg gtaacgtttc gagacggttt ttcagcaaca
    34621 ccaggctttc gcgcgccact tcacgcgctt ctttacggtg caggcggctt tcggcattgg
    34681 tatccaccgg gtcagactct ttcggcccca aatggctgta tgggtcgtta aacaatccca
    34741 tatcatattt aacgttcagt acatggcggg cagcatcgtc cagctcttcc atcgtcactt
    34801 tgccggattt gatcaaccca ggcagatact tcgagtaata ctcgtcgctc atactcatgt
    34861 tgattccgga tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc gtgccatgtt
    34921 taatcagctc tttgattgca ccgtgatcgg aaacggtgat gcctttaaag ccccactggt
    34981 cgcgcagaac atctttcagc agccaagaat cggaggtggc tggcgtgccg ttcagcgagt
    35041 tcagcgccac catcaccgcg ccgctgcctg cgtccagccc cgctttgtac ggcggcatat
    35101 aatcattaaa caggcgctgc ggactcatat cgacggtgtt gtactcttta ccgccttcta
    35161 ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga tctgccgggc
    35221 ttttaccctg catcgcttcc accatggttt tacccattgt tgaggtgaga tacgtatctt
    35281 cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg cgagacatcg accatcggtg
    35341 cccaggtcat attcaggcca tcatctgccg cttcataagc agagacacgt ccgaccgttt
    35401 tcactgcatc gaggttaaaa gacgaggcca gaccgaggct aatcgggaac accgtgcgct
    35461 gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt aattccatca
    35521 cctgatcctg catggcgcgg atatcctgac gggttacggt gttgaaaatc gccccaacct
    35581 gaccgtcttt gatcatctcg cggatcgcct ctttcgggtt atccgggccg acgctgatta
    35641 agcgcagctg accaattttc tcatcaactg tcattttctt aagcagttcg gtgacgaacg
    35701 catcccgcgc ttcgggcgtt aatggatggt tgccgaataa atcatccgcc agtgctggct
    35761 gcagggccag actcaccgcg attcctactg aacatagcca tttcatatgg atttttcttc
    35821 ctctgttgcc cgacgaggca gcgaaaatgg tgcaaaaacc gtagtttgcc ataagcatga
    35881 tggagagaga aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt cactcgctga
    35941 attgtcatac aaggcgctat tctagtttgt gatatttttt cgccaccaca aggagtggaa
    36001 aatgtcttcc atgacaacaa ctgataataa agcctttttg aatgaacttg cccgtctggt
    36061 cggtcattca cacctgctca ccgatcccgc aaaaacggcc cgctatcgca agggcttccg
    36121 ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc tcactactgg aattgtggcg
    36181 ggtgctgaaa gcctgcgtca ccgccgacaa aattattctg atgcaggctg ccaatacagg
    36241 cctgaccgaa ggatcgacgc caaacggtaa cgactatgat cgcgatatcg tgatcatcag
    36301 caccctgcgt ctcgacaagc tgcacgttct cggcaagggc gaacaggtgc tggcctatcc
    36361 gggcaccacg ctctattcgc tggaaaaagc cctcaaaccg ctgggacgcg aaccgcactc
    36421 agtgattgga tcatcgtgta taggcgcatc ggtcatcggt ggtatttgta acaactccgg
    36481 cggctcgctg gtgcaacgtg gcccggcgta taccgaaatg tcgttattcg cgcgtataaa
    36541 tgaagacggc aaactgacgc tggtgaacca tctggggatt gatctgggcg aaacgccgga
    36601 gcagatcctt agcaagctgg atgatgatcg catcaaagat gacgatgtgc gtcacgatgg
    36661 tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt gatattgaag ccgacacgcc
    36721 cgcacgttat aacgccgatc cggatcggtt atttgaatct tctggttgcg ccgggaagct
    36781 ggccgtcttt gcagtacgtc ttgatacctt cgaggcggaa aaaaatcagc aggtgtttta
    36841 tattggcacc aaccagccgg aagtgctgac cgaaatccgc cgtcatattc tggctaactt
    36901 cgaaaatctg ccggttgccg gggaatatat gcaccgggat atctacgata ttgcggaaaa
    36961 atacggcaaa gacaccttcc tgatgattga taagttaggc accgacaaga tgccgttctt
    37021 ctttaatctc aagggacgca ccgatgcgat gctggagaaa gtgaaattct tccgtccgca
    37081 ttttactgac cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt taccgccgcg
    37141 catgaaaaac tggcgcgata aatacgagca tcatctgctg ttaaaaatgg cgggcgatgg
    37201 cgtcggtgaa gccaaatcgt ggctggtaga ttatttcaaa caggccgaag gcgatttctt
    37261 tgtctgtacg ccggaggaag gcagcaaagc gtttttacac cgtttcgccg ctgcgggcgc
    37321 agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa gacattctgg cgttggatat
    37381 cgctctgcgg cgtaacgaca ccgagtggta tgagcattta ccgccggaga tcgacagcca
    37441 gctggtgcac aagctctatt acggccattt tatgtgctat gtcttccatc aggattacat
    37501 agtgaaaaaa ggcgtggatg tgcatgcgtt aaaagaacag atgctggaac tgctacagca
    37561 gcgcggcgcg cagtaccctg ccgagcataa cgtcggtcat ttgtataaag caccggagac
    37621 gttgcagaag ttctatcgcg agaacgatcc gaccaacagc atgaatccgg ggatcggtaa
    37681 aaccagtaaa cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg cagagtatcg
    37741 ccatccgaat tcacttaatc gttctgtgcc gtctgccccg ccgccatttg ggcggctttt
    37801 tgttttttat agctcaacgc tgctgccggc acaggcatca ctttaccggt ttcaatccag
    37861 gtacgcaggc ggctggcatc ggcaaaatgg gtatatttgc caaacgcgtc catcactacc
    37921 agcgccaccg gtttattatt gataaccgta cgcatcacca gacaatggcc cgccgcattg
    37981 gtaaagccgg ttttggttaa ctgaatattc cagttatcgc gatacaccag atgattagta
    38041 ttgcggaacg gcagcgtata cgtcgggtta gagaaggttg ccatatcttc ccgggtagta
    38101 cttaactgcc cgatcaacgg atattgtttg ctggcaatga gcagtttggt taagtcacgg
    38161 gcagttgaaa cgttatgcac cgacaatccg gtaggttcaa caaagcgcgt gttgttcatt
    38221 ccgagcgatt tcgctttcgc attcattgcc ttaataaagg ctttgtaacc accgggataa
    38281 tggtgcgcaa ggcttgccgc cgcgcggttt tctgaagaca tcagcgccag caacagcata
    38341 tctttacggc tgatttcgct attcagtcgt acgcgcgaat agaccccttt catctccggc
    38401 gtctggctga tatccacttt tagtttttca tccagcggca gtcgtgcatc cagcacaacc
    38461 atcgcggtca ttaatttgct gatagacgca atcggacgca ccagatccgg gtggttcgaa
    38521 tagatcactt tgttggtatt cagatcaaca atcatcgcgc taccggaggc aatttccggt
    38581 tgtgaagcgg tggtagcggc tgccgttttc gcaacggcct gcggtgccaa aggcacagcc
    38641 agcatcaggg ccaggctaaa taaagaaact cgaaatttcg gcatgatgag cattcagata
    38701 gtggttcacg cgcacgggtt gcgcaccgcc ggagtaagga tttactgagg ctagcgacgc
    38761 catcataacg agcaaaaagt gcgatcgtca aaggagaatc gtgaggaaat gctgcattgc
    38821 tgacatttac gccagcaatg caacgtcaaa gaactttcta gaacaaacga taaccgtagc
    38881 cccatagtat aacggttagg gcgagcagca cttccagtac cagcacgcca atcgccagcg
    38941 tcgaactaga gaagctaagg ccttcctctt tgttgatatt caggaagctc ggaataccaa
    39001 ggtaaagcag gtagccggtg taaaacagcg ccaccgtgcc gaccagcgca cacaaccaga
    39061 ccagcggata aagcgccacc agaccgctta aaaacagcgg agttgcaacg tagcccgcga
    39121 agaccatgca gtgcgcaagt gacggacgct gcggataatt acgcgccatc caccagatga
    39181 cccgccccat taccgcaacc ccagccagca taacgccata aaacaggaca gccagcgcca
    39241 gtccggtaaa ccaggataac ttcaggatag tgccatcgcc aaaattccag ccaatctgtg
    39301 tagtgccaat gaaggcgcaa atcaccggaa tcgccgccat cagcaaaacg tggtgggtgt
    39361 aatggtgaga aatcgtttcg ttttcgcgat taatcacctg catttcacga tcgggatggg
    39421 aaaacagtcc ccagacatgg ctcataccgc ccccttgttg tgagttcatg aacctgacag
    39481 ttcaagtata agtcagcttg tgattatttt ttgttcgctc atgtaatttg caccgtctga
    39541 tgtacgtcct ttttcagcgt gaatgatttc cagggtgtat gcttacaggc aaccaaaggg
    39601 agacagactg gcctatggat ctcaatacac tcatctcaca atatggttat gccgcgctgg
    39661 tgatcggtag cctagcggaa ggtgaaaccg tgactttgct gggaggcgtt gcggcgcatc
    39721 aggggctatt aaagttcccg ctggtggtac tttctgtggc gcttggcggc atgattggcg
    39781 accaggtgct ttatctgtgc gggcggcggt ttggcggcaa gctgttacgc cgtttctcga
    39841 aacatcagga taaaattgag cgggcgcaga aacttatcca acgccatccg tatctgtttg
    39901 tcattggtac gcgctttatg tatggctttc gggtgattgg cccgacgctg attggtgcca
    39961 gccagctgcc gccgaaaatc tttctgccgc ttaatattct cggcgcattt gcctgggcgt
    40021 tgatttttac cactatcggt tacgctggtg gtcaggtgat tgcgccgtgg ttgcacaatc
    40081 tcgatcagca tttgaagcac tgggtctggt tgattctggt cgtggttctg gtggtaggcg
    40141 tgcgctggtg gctgaaacga cgcgggaaga aaaagccgga taatcaggcg taaaaccatt
    40201 gccctggata aggcgttcgc gccgcatccg acatccggtg caccatgcct gatgcggcgc
    40261 ttgtgcgtct tatcatgcct accaaaattt tacccattga aatatccaca cacctcactg
    40321 cgctattctg ggttgaactg cggattcgcc aacataaagc cgccatccac tatcaacgac
    40381 tgcccggtgg tgtaatttgc gccctccgaa caaagccacg ccaccaggct ggcaatctca
    40441 tacgttgcgc caaaacgccg cagaggaatc gaaggctccg cgtcgggctt cacgtcgcta
    40501 tcatccatgc cgttcattgg cgtagcgatc gccccaggtg cgactgcgtt caccaaaatc
    40561 ttatgcctga ccaactccag cgccattgct ttggttaacc cgccgagcgc atgtttagcg
    40621 gctgtgtagg cgctggcatc cggcagcggc gtatgttcat gtaccgacgt aatgttgatg
    40681 atgcgaccac cctgcccttg tttcaccatc tggcgggccg caatttgtga gcataaaaat
    40741 gcaccatcga catcaacggt aaaaatcttg cgccactcat cgaaagccat atcaagaaac
    40801 ggcgctttgg tcattgcgcc cgcattattc accagcacat caatacgccc cagccgttga
    40861 atgagtttct ccagcgcctg tgcaccttct ggcagtttac cgagatccag ctgcacaatc
    40921 tccgcacgta cgccgtggct aactacctca cgcgcggtat cttttgcccc ttcttcatct
    40981 gagtgccagg taatgccaat atcaaacccc tgctgcgcca gtaataacgc gcactctttg
    41041 ccgatccccg aatcggaggc ggtaataatc gcaacctgtg ccatcgagtt ctccacttaa
    41101 cgctgaataa acgttaagta tagaaggcgc atatcatcag cgtttgtaac ccccgcccaa
    41161 cgcaccagtg agttgaatgg aagcatccag ccactgccct tgcaataaca ggccattggc
    41221 ccgctcacgc agcgcgggga ttctggcttc gctgacgcgg gaaccagcaa tgatgcccgc
    41281 gttaaagcgc gcctgcgcaa gacctaccac acgcaaggca tcgcgctcaa tttgcgcctg
    41341 atgctggttt ttctccgcaa gtgtctgaac ctgactggct gcccgcgcca cgtcattcac
    41401 cgcttcaacc accgctttgt tgtagctggc gatagacaag ttgctttcgg cttttgcgat
    41461 atcgagattg gcgttaagac gaccactatc gaaaatgggt agcgtcaggc ctgccgtaac
    41521 gcccatttgc tgcgcggaat gacggaacag atcgcttaag tgcaacgcat cctgttgcag
    41581 gaaggccatc aggttgatgt caggataaaa tgccgctttt gccgcatcaa tggtgcttag
    41641 cgatgactca acgtaccagt gcgccgcctg caaatctgcc cgccgggcca gtaaggagta
    41701 ccccagttca tcaggaagct ggcttgccac tttcggcaac gcgaccggtt taagcttcaa
    41761 tgactttgtc tggttatttg taagtgcgct taaccgtgcc tcaataattt tcattttccc
    41821 cgcgacatcg ttgagctgct gccgggtttt gctggcatta atatcggttt ccacaccttc
    41881 aactgaagaa gtaatcccgt tctgatatag ctggcgatcg gtcgcgataa tggtgttctg
    41941 ctctttttct atttgctgca agaccgtgtt taacgccgcc tgggtttgcc actcccagta
    42001 caggcgggct acgctgccag ccagcaattg gcgggtttgc tcgcgttccg ccgcccgtgc
    42061 tttaaccgta cccaggcggg cagtaacctc cgcccgattc tttccccaga tatcgagatg
    42121 ccagcccgcc gttaagccaa aagttccgtt ggtgtaccac ggaccggtcg tacctgcggc
    42181 cggatcgttc agagcaaacg gccccattaa gccttctgcc gacatttttt gccgctccat
    42241 atccgcagaa aagtcgatct gcggaccatc ctgagtagca attgccttcg cctgggcttc
    42301 agctagctga atgcgctgtt cagccacctg catatccggt gcgttctgta gtgcattgtt
    42361 aattaaggaa gtgagttgat tatcgtgata ctccagccac cattggctgt ctggccaacc
    42421 atttttcagc gccgtgggta atgcggtgtc aacttgtgca gcgggcgttt gctggcttaa
    42481 tgcctggcgg gtttcatgca taggcgcaca cccggccagc atcagtaaca gcggaaaaca
    42541 ggcgatggcg ggataaaagg aatcacgatt catgggggaa taatcaggta agaaaaggtg
    42601 cgcggagatt accgtgtgtt gcgatatatt ttttagtttc gcgtggcaat acatcagtag
    42661 caataaaacg acatatccag aaaaatatac actaagtgaa tgatatcttc cgatttatct
    42721 taatcgttta tggataacgg caaagggctt cgttttttcc tatacttatt cagcactcac
    42781 aaataaagga acgccaatga aaattatact ctgggctgta ttgattattt tcctgattgg
    42841 gctactggtg gtgactggcg tatttaagat gatattttaa aattaattaa tgtcatcatg
    42901 tccgaaaata acgagaatat ttcagtctct catcctgttg cgctcctgtc atgtgcattg
    42961 cttcatataa tcactggcgc aaggagcgcg cagggggcgg ccaatcgccg ccgccccctg
    43021 cacccccggg ctctggcgaa caaaatcgcc gctgcgcggt gccctcggct tatcccttac
    43081 ggctaccggg tcgggcgcga ggtaacatcc ctgtaaaacg cgccctcagc ccacatccat
    43141 gtgggctgcc ccggccttca gggaacgcct cggcaatttt gacgccacca aacaaccgtg
    43201 cggcctattg ataaagagct aacacattgt caaaaaacat cactatggtt ttttagagtt
    43261 tctcgatatc aattgcctga atagcccttg caatatcagg ggaattattc aacacccgaa
    43321 catgctgaaa taatcccgtt gcttcatcgt attctttacg caaataactc aaccactgtt
    43381 taatccgcgc aacgtgatat aacccggtat cgccctgctt ttccagacgg gtatattttt
    43441 gcagcaaagc aaccacctcc ggccacggca ttcgcggttc gttatatttt actacccggc
    43501 tcaggttggg aatattgagc gccccgcgac caatcatcac tgcgtcgcag ccgctgatcg
    43561 ccatgcattg ttgcgcactc tgccagtccc agatttcacc gttggcaatc accggaatat
    43621 tcagccgctg gcgaatctcg ccaatcgcct gccagtcaat atgctccgcg cggtaaccct
    43681 gctcttttgt ccgcccatgc accaccagct ccgtagcgcc agcctgttga accgcatcgg
    43741 cgatttcaaa tttcttctca ccgctgtccc agcccagacg cacttttacg ctgacgggca
    43801 aatgcgctgg tacagcttca cgcatcgctt ttgcaccctg gtagatgagt tcaggatctt
    43861 tgagtaacgt cgccccgccg ccgctgccgt taaccgtttt cgacgggcag ccgcaattga
    43921 gatccacgcc ccaggaacct aattccaccg cacgggcggc gttctctgcc agccattgtg
    43981 ggaactgacc taacaactga acgcgcacca gcgtaccgga tggtgtccgg ctggcgtttt
    44041 gtagctcagg gcaaatgcga tgaaagactt ttaccggcag cagttgatcc accacgcgga
    44101 caaactcggt gatgcacaga tcgtagtcgt taacttcggt cagcaattca cgcaccagag
    44161 agtcaagcac tccctccatc ggtgccagta acacacgcat atcatcaccc gcaaaataat
    44221 gaggcgctat gttagcgcct atggtcagcg gattaaaggt ctacaaagag ggattgctgc
    44281 aaactgcaat ccctctctat gcttgatgta tgcctgagtt cgttgcgcat tgcgtaatgc
    44341 cggggagcga aaaaataagc gctgatttct taatgtgatc ggtagcacgt tttacgaatt
    44401 aattgtatga tgaatccatc tcatctgggg tgttgattat gagtaagaca ctgaacatta
    44461 tctggcaata tttacgcgct ttcgtcctga tttatgcctg cctgtatgca ggcattttca
    44521 ttgcttccct gctaccggta accattccgg gcagcatcat cgggatgctg atcctgtttg
    44581 tcctgctggc cttgcaaatt cttccggcaa aatgggtcaa tccggggtgc tacgtactga
    44641 ttcgctatat ggcgctattg tttgtgccga ttggcgtagg cgtcatgcaa tattttgatt
    44701 tgctccgcgc acagtttggc ccggtagtgg tttcctgtgc aatcagtacg ctggtggttt
    44761 ttctggtggt gagctggagt tcacaactgg tacacggtga acgtaaagtc gtaggtcaga
    44821 aaggatcaga agaatgatgg cgaatatctg gtggtcatta ccgctgacgt tgattgtctt
    44881 ttttgccgcc cgcaaactgg cggcacggta taagtttccc ctgcttaacc cgttgctggt
    44941 agcaatggtg gtgatcattc cttttttaat gctgaccggc atctcttacg acagctactt
    45001 taaaggtagc gaagtgctaa acgatctgct gcaaccggcg gtcgtcgcgc tggcctatcc
    45061 tttatatgag cagctacacc agatccgcgc gcgctggaaa tcgatcatca ccatctgttt
    45121 tatcggcagc gtggttgcga tggtgacggg gacttccgtg gcattgttga tgggcgcttc
    45181 accggaaatc gccgcgtcaa tcctgccgaa atcagtcacc acgcctattg caatggcggt
    45241 tggcggcagt attggcggta ttccggcaat cagcgcagtt tgcgtgattt tcgtcggcat
    45301 cctcggcgcg gtatttggcc atacattgct taatgcgatg cgtattcgta ccaaagctgc
    45361 gcgcggtctg gcaatgggga ctgcctcgca cgccctcggt acggcgcgct gcgccgagct
    45421 ggattatcag gaaggtgcat ttagttcgct agcgctggtg ttatgcggga taattacttc
    45481 gctgatcgca ccgttccttt tcccgattat tctggcagta atgggctaaa atttgcgatg
    45541 cgtcgcgcat ttttgatgta agtttcacgc gttgcataat taatgagatt cagatcacat
    45601 ataaagcctt aacgggttcg taaactgtta tcccattaca tgattatgag gcaacgccat
    45661 gcatccacgt tttcaaaccg cttttgccca acttgcggat aacttgcaat ctgcactgga
    45721 acctattctg gcagacaagt acttccccgc tttgttgacc ggggagcaag tctcatcgct
    45781 gaagagcgca acggggctgg acgaagacgc gctggcattc gcactacttc cgctggcggc
    45841 ggcctgtgcg cgtacgccat tgtcgaattt taatgttggc gcaattgcgc gcggtgtgag
    45901 cggaacctgg tatttcggtg ccaatatgga atttattggt gcgacaatgc agcaaaccgt
    45961 tcatgccgaa caaagcgcga tcagccacgc ctggttgagt ggtgaaaaag cgcttgcagc
    46021 catcaccgtt aactacacgc cttgtggtca ctgccgtcag tttatgaatg aactgaacag
    46081 cggtctggat ctgcgtattc atctgccggg ccgcgaggca cacgcgctgc gtgactatct
    46141 gccggatgcc tttgggccga aagatctgga gattaaaacg ctgctgatgg acgagcagga
    46201 tcacggctat gcgctgactg gtgatgcgct ttctcaggcg gcgattgcag cggcaaaccg
    46261 ttcgcacatg ccttacagta agtcgccaag cggtgtcgcg ctggaatgta aagacggtcg
    46321 tattttcagc ggcagctacg ctgaaaacgc cgcattcaac ccgactctgc caccgttgca
    46381 gggagcgtta attctattga atctcaaggg ttatgattac ccggatatcc agcgcgcggt
    46441 tctggcagaa aacgccgatg cgccgttgat tcagtgggat gccacctccg caacgctgaa
    46501 agctctcggc tgtcacagta tcgaccgagt gcttctcgct taagcctggt gccggatgcg
    46561 gcgtgaacgc cttgtccggc ttgccagccc tctcctggtg tcgaaattcc cggcaaacag
    46621 tttgccgttt cttgcgcaaa accagcgggt aaagtagcct gatggaaatt ttccttagat
    46681 cgagtctcct gcatgttaaa gcgcgtgttc ctcagcctgt tagtcctgat cggcttgctg
    46741 ctgttgactg tgctcggcct cgatcgctgg atgagctgga aaaccgcgcc ttatatctac
    46801 gacgaattgc aggatctccc ctaccgccag gtcggtgtgg tgctcggaac agcaaaatat
    46861 tatcgtactg gcgtaattaa tcagtattat cgctaccgca ttcaaggagc gattaatgcc
    46921 tataacagcg gtaaggtaaa ttatctatta ctgagcggcg ataacgcatt gcaaagttat
    46981 aatgagccga tgactatgcg caaagattta atcgctgctg gtgtcgaccc atcagatatt
    47041 gttctcgatt acgcaggctt tcgtacgctg gattccatcg tgcgtacacg caaagttttc
    47101 gatactaatg atttcattat tatcacccaa cgtttccact gtgagcgggc attatttatt
    47161 gcgctgcata tggggattca ggctcagtgt tatgccgtac cgtcaccgaa agatatgctg
    47221 tcagtacgta ttcgcgaatt tgccgcccgt ttcggtgcgc tggctgacct ttatattttt
    47281 aaacgtgaac cgcgtttttt agggccgctg gtccctattc cggctatgca ccaggtgccg
    47341 gaagatgctc aggggtatcc cgccgtcaca cccgaacagt tacttgaatt acaaaagaaa
    47401 caaggaaagt agttatggat gtacagcagt tttttgtcgt tgccgttttt ttccttattc
    47461 cgatattttg tttccgcgaa gcatggaaag gctggcgcgc aggcgcgatt gataaacggg
    47521 ttaaaaatgc accggaaccg gtgtatgtct ggcgagcaaa aaatcccgga ctctttttcg
    47581 cttatatggt ggcatatatc ggcttcggaa ttttatctat cggcatgatt gtttatctta
    47641 ttttctatcg ttaatatttt ctcttttctg ttaattattg agaataatta ttacttcact
    47701 caataagctg cggatataac attctgaacc gcagctattt gtgaatcctt tcacagtata
    47761 aatttcccca cgcgcatagc cttaatatca gcacgcaata tttatactct aaataattcg
    47821 agttgcagga aggcgacaag tgagtgagtc cccaggagct tacataagta agtgactggg
    47881 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat ttagcgttcg
    47941 ccttaattac tcatagcatt aaggaagatc acatgccgca acaaaattat ctggatgaac
    48001 tcactccggc ttttacgcct ttactggcga ttaaagaagc ctctcgctgt ttattatgtc
    48061 atgacgctcc ctgtagtcag gcttgcccgg cacagaccga tccggggaaa tttattcgct
    48121 caatctactt tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat aatgccctcg
    48181 gtgccgtttg tgccagagtg tgcccgacgg agaaattatg tcaaagcggt tgtacccgtg
    48241 ccggtgttga tactcccatt gatatcggtc gcttacagcg ttttgttact gattttgaac
    48301 aacaaaccgg gatggaaatt tatcagcccg gtactaaaac gctcggcaaa gtcgcgatta
    48361 ttggcgcagg tcctgccgga ttgcaggcca gtgtaacact gacaaaccag ggttatgacg
    48421 tcacgattta tgagaaagaa gcgcaccccg gtggctggtt gcgtaacggt attccgcaat
    48481 tccggttacc gcaatcagtg ctggatgcag agatcgcccg tattgaaaaa atgggcgtga
    48541 ccattaagtg caacaacgaa gtcggtaaca cactcaccct ggagcaactg aaagcagaaa
    48601 accgcgcggt actggtcacc gtggggttat cgagcggttc cgggttatcg ctgtttgagc
    48661 atagcgacgt tgagattgcc gtcgacttcc tgcaacgtgc acgacaggcg caaggcgata
    48721 tcagcattcc acaaagcgca ttaattatcg gcggcggtga tgttgcgatg gacgtagcca
    48781 gcacgctgaa agttctcggc tgtcaggcgg taacctgcgt agcgcgtgaa gagttagatg
    48841 agtttccggc aagcgaaaaa gagtttgcca gcgcccggga gctgggcgtt tcgatcatcg
    48901 atggattcac gccagtagcc gtggaaggca ataaagtcac gtttaagcat gtacggttac
    48961 cgggcgaact gacgatggcg gcagataaaa ttattctcgc cgtcggtcag catgccagac
    49021 tggatgcctt tgcggagtta gagccgcagc gtaacaccat caaaacacaa aattatcaga
    49081 cccgcgatcc gcaagtcttt gctgctggcg atattgttga gggtgacaaa accgtggtct
    49141 atgccgtgaa aaccgggaaa gaagccgccg aggcgattca tcactattta gagggagctt
    49201 gctcatgtta acgaaagatc tttcgattac tttttgcggc gtgaagtttc ccaacccgtt
    49261 ctgcctctct tcttcgcctg taggcaactg ctatgagatg tgtgccaaag cctacgacac
    49321 aggttggggc ggcgtggtgt ttaaaacgat cggctttttt atcgccaacg aagtctcgcc
    49381 gcgttttgat catctggtga aagaagatac cggttttatc ggcttcaaaa atatggagca
    49441 gattgctgaa catccgttgg aagagaatct ggccgccctg cgtcagctga aggaagatta
    49501 cccggacaaa gtattgatcg cttcgatcat gggggaaaat gagcagcaat gggaggagct
    49561 ggcgcgcctg gtgcaagaag ctggcgcgga tatgatcgag tgtaacttct cctgtccgca
    49621 aatgacttct catgcgatgg gtagcgatgt cgggcaaagc ccggagctgg tagaaaaata
    49681 ttgtcgggca gtgaaacggg gttccacgct gccaatgctg gcgaagatga cgccgaatat
    49741 cggtgatatg tgcgaagtgg cgctggcggc gaagcgcggc ggcgcagatg gcattgcggc
    49801 gattaacacc gttaaatcca tcaccaatat cgatcttaat cagaaaatcg gtatgccgat
    49861 cgttaacgga aaatcgagta tttccggata ttccggtaaa gcggtaaaac cgatcgccct
    49921 gcgcttcatt cagcagatgc gcacccatcc agaactgcgc gatttcccaa tcagcggtat
    49981 cggcggcatt gaaacctggg aggatgcggc tgagttttta ttgctcggcg cagcaacgtt
    50041 acaggtgacc accggcatca tgcagtacgg gtatcggata gtggaagata tggcaagcgg
    50101 gttgtcgcat tatctcgccg atcagggatt tgattcgctg caggagatgg taggtctggc
    50161 gaataacaat attgtcccgg cggaagattt agaccgcagt tatattgtct atccccgtat
    50221 caatcttgat aaatgtgttg gctgtggacg ctgttatatt tcctgttacg acggcggtca
    50281 ccaggcgatg gaatggagcg agaaaacccg cacaccgcat tgtaataccg agaaatgtgt
    50341 gggttgtctg ctttgtggtc acgtctgccc ggtgggttgt attgagctcg gggaagtgaa
    50401 gtttaagaaa ggcgagaaag aacacccggt aacgttgtaa aaccagagac gcatccggca
    50461 tttggcaccg attgccggat gcagcgcatc cggcaatatc cacaatcaca tcacttctta
    50521 cgcgcgtatt tcagtgaatc cagcgctacg gcgaagataa taatcgcccc tttgatgatg
    50581 tactgccagt atgggtttac gccgatatac gtcaggccat agttgatgac ggtaaaaata
    50641 attaccccag tcaccacgcc aatcaccgtc cccacaccgc cgctgaacga tacaccgcct
    50701 accacgcacg ccgcgatagc atccagctca tacataaagc cgaggttgtt ggtggcagag
    50761 ccgatacgtc cggcttctaa catcccgcca aaggcataga acacgccaga caacgcgtag
    50821 atcatcagca ggttcaggcc gacgttgaca ccagatactt ttgccgcttc cgggttaccg
    50881 ccaatggcaa aaatgttctt accgaagcgg gttttgttcc acaacaccca gacgaacgcc
    50941 accgcaatca acgcgtagaa ggtgatgtaa gagagacgga aactccccag cgcgacaaag
    51001 ccctgagcaa aggtagaaaa gccactgtca aaaccagaaa ttggcgacgc cccgacaaag
    51061 tcgtaataga gcgagttgat tccatagacg atgatcatcg tgccgagcgt ggtaatgaac
    51121 ggcgtcacgt tgagataagc gataatcaga ccgttgatca aaccgatcac cgcaccaatg
    51181 gcacagacaa tcagaataac cagcgcaatc ggcatcgtcg ccatttccgg gaacactttg
    51241 ttggcgttat ccatggactg caataatgtc gcagccacca ctgccgccag ccctacctga
    51301 cgaccagcag aaagatcggt cccctgggtg acaattaacc ctgccacacc gagcgcgata
    51361 ataatacgca ccgatgactg ggtgagaata ttacttaagt tcaacagact taaaaatgtt
    51421 gggtcctgga aaataataat cgccagcaaa actaaaagaa cgacgtaaat accgccctct
    51481 ttcaggtaag taagaaaact tttcttattt aacgcactca tgggaagccc ctaatcttaa
    51541 aggtgcaaag acgcaagacg cagaatttcg ttttgcgttg ttgtttttgt atcgacaatt
    51601 ccggaaacga gaccattgct catgaccaga atacggtctg ttatccctaa caactcaggc
    51661 atttcagagg agataataat aatccccttg cctttcttcg ccagttcggc aattaactga
    51721 taaatttcaa acttcgcccc gacatcaata ccgcgcgtcg gttcatcgag cattaatatt
    51781 tctggttgcg ttagtagcca gcgaccaata atcacctttt gctgattacc accggagagc
    51841 gaaccaattt gcgtccgatg acccggcgtt tttacccgca tcgaatcaat cacccactgg
    51901 gtatcgcttt tcatccgcga gttatccagt aaaccaactt tatttttgta gttgcgaata
    51961 ttggaaatta acgagttaaa accaatatcc agataggcat aaattcccgt tgagcggcgc
    52021 tcctcagtta ccagtgcaaa tccgtggttt atggcttcgt tggcattatg gttattgatc
    52081 tgtttgccgt gcaacgtaat ggtgccagcc gatttctcgc gaataccaaa taacgtctca
    52141 acaatatcgg tacgtttcgc ccccaccagc ccggcaatac cgaggatctc ccctttatgc
    52201 agatcaaacg agacatcgcg aatcgacggc tggcgcagtg acgttaggtt acgcacctcg
    52261 aggatgactt cgcccggctt gttttctttg tcaggaaaac gctgattaag agaacgccca
    52321 accatcatgg cgatgatctt gtccatcgtc agtcctgcca gcggttcggt ggcgatccac
    52381 tgaccgtcgc gcaatacggt aacttcatca cataactgga agatttcttc cattttatgc
    52441 gagatataaa caataccgca gccgcgctct tttaatttac gaataatagt gaacagatga
    52501 ttgacctctt tttcggttaa cgaagaagtc ggttcatcca taatcacaat tttcgcgtta
    52561 taggaaaacg ctttggcgat ttcgatcatc tgcatttgcg aaacggataa tgtgccgacg
    52621 cgcgcacgcg gatcgatatc aatatccagt tcatcaaaaa tcgctttggt ttcgcggtac
    52681 attttgtcct gatcgacaaa catgcctttg gtgggatatc gccccagcca catgttatcc
    52741 atcaccgaac gttgtaatac caggtttaac tcctggtgta ccatcgaaat accattttcc
    52801 agtgcttctt tggcggaatg gaaatcgatc tctttaccct ggaataaaat ggtgccggag
    52861 tctttttgat aaataccaaa caggcatttt aataatgttg attttcctgc gccgttttcc
    52921 cccattaatg catggataga atgtggccgg acttttaaat taacgttatc aagtgcctta
    52981 acaccaggaa aagacttgtt gataccgctc atttccaaca agtattcccc ggaagacgga
    53041 gtcgttgagc tgaccatata attttacctt gttggccata caataagggc gcagtaaacg
    53101 actgcgccca atcagtctta tttcttgctg aattcagcca ggttgtcttt atctacgcca
    53161 acataaggta cgcggaccac tttgttgtcg attttccagt tggtgccatc agccgcacct
    53221 ttgccatcgg ccaagttttt cgccagatca aaggtcgctt tcgcctggtt gttagcatcg
    53281 ttcagtactg tgcccgccag tgcaccggat ttcaccagcg ccagcgcttc tggcagcgca
    53341 tcgacgccaa acaccggaat gctggacttg ttgtgtgctt tcagtgcttc taccgcgccc
    53401 attgccatcg catcgttgtt ggcgataacc acttcgattt tgttggcgtt cgggccagac
    53461 agccaggcgt ccatcttatc tttcgcctga gcggtatccc acatagcggt atctaactgt
    53521 aactgttcag ttttaatgcc tttgtcgttc agctctttaa tcacgtaagt ggtacgtgct
    53581 tctgcatccg gatggcccgg ttcacctttc agcaatacga attgaatctg accgtctttg
    53641 ttcagatccc aaccctgatt cgccgcccag tgtttagcaa tcaaatcgcc ctgaataatg
    53701 ccggactctt tggaatcagt gccaacgtag taggctttgt cgtagctatc cagcgcctta
    53761 cgagacggtt ctttgttgaa gaaaaccacc ggcacgtttt gcccacgcgc tttctcaatc
    53821 accgtacccg cagctgccgg gtcaaccagg ttgattgcca gtgccttcac ccctttcgcc
    53881 agcaatacgt cgatctgatc gttctgcttg gactggtcat tctgagaatc attcatcagc
    53941 agctgaacat ctggcgcggc tttcgcatct tgctcaatag ccttgcgcac tacagacata
    54001 aagttatcgt cgtacttata gattgttaca ccaatgcgag tatcagcagc gtgtgcagcg
    54061 gcaccgaata acatgctggc catcacagca gacagggtta acaccttctt attcatggta
    54121 tctccggttt ttcttatgca gggtagtgct tgagataaat gctcggcggg gcagtagagt
    54181 taatgaagtg ttactgaacg ccgaagctca ctttttaaaa ttcgttcttc catgcccggt
    54241 aacgctccag aaaacggctt taattgttgt tatgacgcta ttacctcggt gaaagtgatt
    54301 attcactgtt acatacgggt tacaacgtta aaacggtgca atcatagcta tcacattgtt
    54361 aagatactgt gaaatcactc acagattgaa agcggttaca tcgcctgatt tgttgagtta
    54421 gtgatcgtcg ccgcattctg gcgtgttgcg acagaatggc gacgcactaa cgtcggcata
    54481 aaacagtggc tggcacgagg atcaatattg cctgctgccc cctgcaaggc cagttcggtg
    54541 gctaatttcg ccattgaagc aatgggataa cgcacggtcg ttaattgcgg gtcggtgtaa
    54601 cgggcgatgg gaatatcatc gaaaccgatg attgagagat gtaacggaat cgcaatgcca
    54661 ttatctttta atgctgtcag tgcgccagcg gccatattgt cgttataagc aaatacagcg
    54721 gtaagttgta gattgcgccc cagcagttca accatcgccg cctcaccgcc cggcatgtcc
    54781 ggcgtaccag tgccaatcca gctttccggc ggaataatat cctgctcttt caacgcactc
    54841 atccagcctg ctttacgcat ggcgtcatct tcaatgccgt ggctggaaga aagataacca
    54901 atacgttgat gaccgttatt cagcaacatg cgcgtcgcca ttcgggcacc gctgagatta
    54961 tccaggcaaa cgcaacgatg ggcgtacccc ggcacaacgc ggttgattaa caccataccg
    55021 ggaatgttat ccataaattg cgccagttca tcgtcactca atgcttttga gtgaacaatc
    55081 aacgcattac aacgctggcg aattaacacc tcaatggcgt gacgctcttt ttccgcttca
    55141 tgatagctat tgccgattag cacgtatttc tgatgctgct gggcgaccag atccaccgct
    55201 tttaccagcg cgccgaaaaa agcatcagaa acgtccatca ccaccacgcc aatggtatcg
    55261 ctaacctgag ttgccagcgc ctgggcattg gcatttggcc gataatccag ctcactcacg
    55321 gctttcatta ctgcttcacg cgtgtcggca ctgaccagcg tgctgttatt gagcacccgg
    55381 gaaaccgttg ccacagagac gccagcctga cgcgctacat cacgaatggt gatcatattc
    55441 actacctgtt atgagattgc agtaaatgac tgcttgctgg cggctatttt gtcagcactg
    55501 aatacaggac ttcgtgaatc gagtcacagc aatggaaacg gttacagccg ttttgttaac
    55561 gaatgtgatc cagatcgtta tcttgatgtt ttagatattg ccgttccggc agcacgcaaa
    55621 gttaactgac gccagagcca ttccaccggc ccctggcgga agtaacgcag ccagataaca
    55681 gagaagagga tattcgccag ccataccgga ataacaaacg ccagcagctc caggcgatca
    55741 aaatgcataa acaaaccgag gtggtaaaaa agcgtggtac aaatcagcgt ttgcaataga
    55801 tagttggtca gcgccatccg tccgacgcag gcgattgcca gcaccagctt aaagcggctt
    55861 aattgcggcc agaagccata aaacagcgac gcatagccaa tcgcctgaaa cggcgcactc
    55921 agttcccgcg gcatttgaag taagaaggca caccagcgat acgcccagtc cagctgccat
    55981 tgcagggcga tggcaggaag gttaatggtc accccaatcg ccaccagcac aaaaccagta
    56041 cgacgatagt gacgtaagct gaactgccct ttcagccagc cgctgcgcat cagcgccgca
    56101 ccaatgagca tcatcccagc cagttgccag ccatactgtg cgcccagtgc cagtaaactg
    56161 ttgccaacac catcggcacg attactgatc gcatcaacgc cgccgtgaag cttccagtat
    56221 ttttcataca aaatagccga tgcatccggc gtccaggcgc ggctggtctg gctgtcggaa
    56281 atcagcccca ataacagcaa aacgccaagc cccaccagat aaagcatgac gccggtatta
    56341 aacaggcttt ttaccgatgg cgcatcgcgc accagccgcc agcagattaa gcccaccagc
    56401 ccgtaagcca gcagaatatc gccgtcccag aacaataaac cgtgaataaa gcccaataag
    56461 actaacagcg ttaaccgcga ctggatccag cgtctgccac ggggcagcaa catttgcagt
    56521 ccagcaccaa acagcagcgc aaaaagcgtg aggaatttca cctggccgat gagatcaaga
    56581 aatgcccagg tccatgcatc ctgcggcgta atagcaccgt accaggcggg attgagataa
    56641 gccgcctttg gtagcccaaa ggcgctgatg tttaatagca ggatccccag aatggcgacg
    56701 ccgcgaacaa aatcgagcgt gacgttgcgc tccatggttc ctgcctttta atcagttgtg
    56761 atgacgcaca gcgcgcagaa actcgtggcg cgtattctga ctggatttga acaatccacc
    56821 aagagaggtc gttgttgtgg cactggttgc atcgcggatg ccacgcgcct tcacgcagta
    56881 atgcaccgcg tcgatcgaga cagccacgtt attggtgccc agcagcgttt gtagcgcaat
    56941 aagaatttgc tgcgtcagac gttcctgcac ctgcggacgc tgggcaaaga actgcacgat
    57001 acggttaatt tttgacagac cgatcaccga atctttcggg atataagcca ccgtcgcttt
    57061 gccatcgatg gtaacaaaat ggtgttcaca ggtactggtc agagtgatat cgcgcacggt
    57121 gaccatttca tcgaccttca ttttgttttc aatgagggtg attttcggga agttggcgta
    57181 atccagaccg gagaaaattt catcgacata cattttagcg atgcgatgcg gcgtttccat
    57241 caaactgtca tcagccaggt cgagattcag cagctgcatg atttcggtca tatgaccagc
    57301 aataaggctt ttgcgcgttt cgttatccat ttcatgcacg ggcgggcgca gcggtgtttc
    57361 cagtcctcgc gcaactaacg cttcatgaac cagggccgct tctttactga gtgatggcat
    57421 ttatgatttc tcctgcaggt gtgacgcctc cgccctgcgt gggggcaaag ttattaagct
    57481 gatttacagc ctgattattg tgcgtgaggc ggcgcacata atccagtatt cacagcgata
    57541 attattgtaa ttgccgctgc ctttcatcag cagatgttaa aacatcgtta tgcaaatacg
    57601 gaagtgaaag ttactcacag cacattgaat aaacggtatg atgaagaaat tgcaaacaac
    57661 acaacaagga gccacgcatg gaaatgctcg aagagcaccg ctgttttgaa ggctggcagc
    57721 aacgctggcg acacgactcc agtaccttaa actgcccgat gacgttcagt atctttctcc
    57781 caccccctcg tgatcacacc ccgccaccag tgctgtactg gctttccgga ttaacctgca
    57841 atgacgagaa cttcaccacc aaggcgggtg cccagcgcgt ggcggcagaa ctggggattg
    57901 tactggtgat gccagacacc agcccgcgcg gcgaacaggt tgccaacgat gatggctacg
    57961 atttaggcca gggcgcgggg ttttatctta acgccacgca accgccgtgg gcgacgcatt
    58021 accggatgta tgattatctg cgcgatgaat taccggcgct ggttcagtcg caatttaatg
    58081 tcagcgaccg ctgcgccatt agcggtcatt caatgggtgg tcacggtgcg ctgattatgg
    58141 cgctgaaaaa tccgggtaaa tacaccagcg tttcggcctt tgcgccaatt gtgaatccgt
    58201 gcagcgtccc gtggggaatc aaagcgttta gcagctattt aggtgaggac aaaaatgcat
    58261 ggctggaatg ggacagttgc gcgctgatgt atgccagtaa cgcgcaggat gcgatcccga
    58321 cgcttatcga tcagggcgat aatgatcagt ttcttgccga ccagttgcaa cctgcggtac
    58381 tggcagaagc cgcgcgccag aaagcgtggc cgatgacgct gcgtattcag ccgggatatg
    58441 atcacagtta ctacttcatc gcctctttta tagaggatca cctgcgcttc catgcgcagt
    58501 atttactgaa gtgaaagtcc gcccggttcg ccgggcatct tctcatcaga agcgataatc
    58561 cactgccata aagtaacgac gtccgtcttc gttatagctg tagtcgtcac gactgagatc
    58621 cttgtcgcca aggttcagca cgcctgcgcg cagtttgacg tctttagtca cctgccaggc
    58681 cgcgccggta ttccagatgg tataaccgcc cggtgtttta gccgtcgcgc tgtcggcgcg
    58741 tttctgcccg gtatagtgac cagaaacata gaatgaccag tcttccagcg ccagcggttt
    58801 ccagtccagc gtaccgttag cagtatggaa cggcagatcg gatagcggtt tgttttcgcc
    58861 gttgctgaca tcacgaccat cgttgtaggt gtagttgatc gacagtttcc attcatcgtt
    58921 gaacggaatt ttcagttcgg tttccacgcc ctgaatacga gctttgttaa cgttgtagta
    58981 gctaaatacc ggtatgcgcc gtccgttagc gcccgtctca aaaccaacaa agttttggta
    59041 gcccggtgca gcgttgacgt cagacgtacg gctgatgctg atacgatctt tcacatcgtt
    59101 acggaaaacg gtaacgctgg attcaacccc ttccagccag ccttcttcac ccatgtagta
    59161 aagccccagc tcccaacttt cgctggtttc tggtttcaga tccgggctac ccacaatctt
    59221 acatgcgcca cggcaggaat tgctcgtcca gtcagggcta agttgcaaca gagaaggtgc
    59281 tttaaatgcc gtcgcccagc cccctttcac cgttacggtg tcggtggcgt tataaaccag
    59341 gtaggcacgc ggactccagt gttcaccgta ggtttcgtga tcgtccatac gcacgccggt
    59401 cgtcagcgcc agcggctcga agatccgcca ttcatcttcc acaaacagcg cgtactggct
    59461 ggcagacgtt ttggagctgg ttcccccggt caggttcacc gcatcgctaa gtttgtcgtg
    59521 acgccattca ccgccaaccg tgagaaactg attaattgcc gtcagcggca acgtgtattt
    59581 gccgtcgacc gcattgcttt cggaagttat cgggctgctg ttgccagggt ttttgttctc
    59641 gactttctca ccgtagtatt tcagttcgct ggtgccgtaa tcccaacgcc cattatggct
    59701 gacggagtag ttctggcgtt ccaggcggtt tttgtccagc gagtcggaat cacgatcctg
    59761 acggtcgaaa ccgtatccgg cagtaaaatc gtgattttga ttcggtgtcc aggcaaattc
    59821 gacattgccg tcgcggctgg agaatccttc aatacgcggc gtttctccgg tatcggtggt
    59881 cgttgagttt tgcgggtcat ccttttcacg ttttgccagg ctgccgtaag ctttcattcc
    59941 cagcacacca tcaattaatg gtccactggt aaagaactga ccgttatagg tatcaccgcg
    60001 atcgcgatgt tcctgaatgg tggtatcgac ggtaacggta cccgaccatt tctgaccgat
    60061 ttttttggtg atgatattca ctacaccgcc gagcgcatcg gagccgtata gcgacgacat
    60121 cgggccacga accacttcaa tacgttcgat ggaatcgacc gggatccagt tcagatcgaa
    60181 atcattgtgg cggaagacgg cattgcggga gttaacgcgt ttaccgtcga cgagaatcag
    60241 ggtgtagctg ctgtccagac cacgaatact aacgccctta cggttatccc cttcgttcgt
    60301 cagttgtacg ccaggcactt ctttgaggac atccttcaga ttctgtaccg gttttcgctg
    60361 caggtcttcc tgggtaatga cgctgatact ggcgggtgca tctttgaggt tttgttcaac
    60421 ggaagatgca gtgacaacca tcgtttcgcc atcatcatcg accgctaaca caggccatgc
    60481 acaagaaata gcggacaaac acagcccgac ccgtacgaaa gggttcaacc taaacattcc
    60541 atatctccat gaggtaacta cgaaaataaa atgggttatc gctcacatct tcttcacgtc
    60601 cccttgcgta cggcagcatc gcggtggact tatttgtatg caggtgatca tcccagaaag
    60661 ccggaacagc ctctgatttg tcagcctttt ttgattgcgg cgtaacgata atgcaaacga
    60721 taacaattat caatccaaat gttaaatttt atatccgcat gatttgtcag ggaaatggaa
    60781 ttgaaaaagc cctctcggtt gagagggctt agcaaggaag ggaggaaact tatttcttat
    60841 cgttctgcgg gaacttcatt tcgctgtaac gtacgaagtg agttccttta atcagcttgt
    60901 agccgaacca aataatcagg aacagcggga taccaatata cgttgccgct acgccgcccc
    60961 agtcaatggt atctttcagg aacgcttcgt agttctggcc caaagtgata atcagacaca
    61021 gaatgaatgc gaagatcggc cccagtggga agaaacctga acggtacggc agatcgttaa
    61081 tgtcgtgtcc ctgcaatacg taaccgcgac ggaagcgata gtggctaatg gcaatcccca
    61141 gccaggcgat aaaacccgtc atcccggagg tgttcagcag ccacaggtat accgtctggt
    61201 tgccaaacat ggaggtcagg aagcacagac cggcaatcac cgtcgtcgca tacagcgcat
    61261 tacgcggcac gccaccacgc gacagtttag cgaaaatgcg cggcgcttta ccgtcacacg
    61321 ccagggtgta cagcatacga gtagacgcat acataccgga gttacccgct gacagcaccg
    61381 ccgtcagaat aactgcgttc atcaccgccg ccgcagagag cagacccgcg tgctggaaca
    61441 ccagggtgaa cggactaacg ctgatgtctt taacatcgtt acgcagcagg ctcggatcgg
    61501 tgtacggaat aatcaggctg ataatcagga tcgcgaacac atagaacaac aggattcgcc
    61561 agaacacctg acgtaccgcg cgtggaatgt ttttcgccgg atcttcggac tcgcctgcag
    61621 caataccgat cagctcggtt ccctggaaag agaagccgac aatcatagct acgccgatca
    61681 tcgccgcaaa accaccagca aacggcgctt cgccgattgt ccagttgctc cagcccgcag
    61741 gctgcgcgcc tttgaagata ccgataatca tcagcacgcc aacgatgata aagacaataa
    61801 ctgtcgtgac tttgatcagt gagaaccagt attccgcttc accaaagcca cgaactgaga
    61861 tgtagttcag caggaagata acgccgagga acaacgcact ccagatccag cccggtgtat
    61921 ccgggaacca ccagctcatg accagctgag ctgcaaccag gtcaacggcg atagtcaccg
    61981 cccagttgta ccagtagttc cagcccagcg cgaagccaaa gccttcttca acatagttct
    62041 gaccgtaagt ggcaaacgaa ccggaaaccg gcatatatgc agccagttca ccgagactgg
    62101 tcatcaggaa gtaaaccatc aggccaatca gcatatacga gagcaatgcc ccgcccggac
    62161 ctgcctgaga aatcgttgcg ccagaggcaa caaaaagacc tgtaccgatg gaaccgccaa
    62221 tggcaatcat cgtcaggtga cgcgccttta attcacggcg taagcccggc gcttctgtgg
    62281 ttttagtttc ggaaaccata caaaaatgct atccatctta aaaatgaggc gcgattgtag
    62341 cagacgattg gcaatccttc cggcagaaat acacggttat aagacacctt catgatcgcc
    62401 cagggattat aagtaaagca gccaatctct tttctggcga atgcatgctg agggcgctgc
    62461 gtcacgaaat cgacacagca ccagcatgtt cttgtacagc aacttaacgc ggtacattcg
    62521 cgggatcgca atagtccaga aagcgccgta gcgcgttgga aaggtgtttt tgccgatgat
    62581 gtatacgcca caacgtacgc atcaggcgcg gcagagggac cgcaacttca cttaatgtgc
    62641 ctgcctgcaa ttgatcttca atcacccgtc gcgacaggca actaattccc aacccatgac
    62701 gcaccgcatg tttgattgcc tcggagttac ctaattccat cgccatctca aacttcggta
    62761 aatgtgacag caatagataa tcgacaatct cccgcgtgcc ggaaccgcgt tcacgcagga
    62821 tccacggcgc agcggccagc tgctctaagg tgaccggacc acgggccaac ggcgaagtcg
    62881 gcgcggcgaa aaccaccagc tcgtcttcca gccacggttc agaaataatt tcagtgctgt
    62941 ggcacggtcc ttcaataaaa ccaatatcaa cgcggaaatc cagcaccgct tgcatcacgt
    63001 cctggctgtt cccaacgcta agttcaatcg gcaactgcgg ataatcatgg cgataacggg
    63061 cgataactgc aggcagaatg tagttaccga tggtactact ggcatagata cgaatcgcgc
    63121 cgttgtcttc gcgaaacagt tgttcgattt caaccgcctg ttcaagcaat gccaacgcac
    63181 gcggatagag cagccgcccg tgttcattaa caaccagtct tttccccacg cgatcaaaca
    63241 gttgcacgcc aagctgccct tccaggtcgg tcaaggctgc gctcactgct gattgcgaca
    63301 acgccagcat caccgacgcc tgggtggttg atccactttt caatacttct gcaaaaactt
    63361 ccaactgccg gagggtgatg tgcatagtcg cttaccactt ataaagatta attataaata
    63421 tataatcaat tttattttta aaccagttag tcgtaacctt atgcccggtt aaaggagagg
    63481 gttatgacga atatcacctt acagaaacaa catcgtacac tgtggcattt tattccgggg
    63541 ttagccctga gtgcagttat caccggggtc gccctgtggg gtggttccat tcccgcggtt
    63601 gcgggtgccg ggtttagtgc cctcaccctc gcaatcttgt tggggatggt tttaggcaac
    63661 accatctatc cgcacatctg gaaaagctgt gacggtggtg tgctgtttgc caagcaatat
    63721 ttattacgtc tgggtatcat tctttatggc ttccgtctga cgttctcgca aattgccgat
    63781 gtcggtatca gtgggatcat cattgacgtg ctgacgctgt ccagtacctt cctgctggct
    63841 tgcttcctgg ggcagaaagt gtttggtctg gataagcaca ccagctggtt gatcggtgcc
    63901 ggtagcagta tctgtggtgc tgc
//
LOCUS       DAFATA010000015        61144 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFATA010000015 DAFATA010000000
VERSION     DAFATA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.015"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="LGJ20_001308"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="LGJ20_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBK2385402.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="LGJ20_001309"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="LGJ20_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBK2385403.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="LGJ20_001310"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="LGJ20_001310"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBK2385404.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="LGJ20_001311"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="LGJ20_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBK2385405.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3241..4635
                     /gene="ychO"
                     /locus_tag="LGJ20_001312"
     CDS             3241..4635
                     /gene="ychO"
                     /locus_tag="LGJ20_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBK2385406.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSLWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4636..5286)
                     /gene="narL"
                     /locus_tag="LGJ20_001313"
     CDS             complement(4636..5286)
                     /gene="narL"
                     /locus_tag="LGJ20_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBK2385407.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPAITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5279..7075)
                     /gene="narX"
                     /locus_tag="LGJ20_001314"
     CDS             complement(5279..7075)
                     /gene="narX"
                     /locus_tag="LGJ20_001314"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBK2385408.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7414..8805
                     /gene="narK"
                     /locus_tag="LGJ20_001315"
     CDS             7414..8805
                     /gene="narK"
                     /locus_tag="LGJ20_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBK2385409.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9198..12941
                     /locus_tag="LGJ20_001316"
     CDS             9198..12941
                     /locus_tag="LGJ20_001316"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBK2385410.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLTGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            12938..14476
                     /gene="narH"
                     /locus_tag="LGJ20_001317"
     CDS             12938..14476
                     /gene="narH"
                     /locus_tag="LGJ20_001317"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBK2385411.1"
                     /translation="MKIRSQIGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14473..15183
                     /gene="narJ"
                     /locus_tag="LGJ20_001318"
     CDS             14473..15183
                     /gene="narJ"
                     /locus_tag="LGJ20_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBK2385412.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15183..15860
                     /gene="narI"
                     /locus_tag="LGJ20_001319"
     CDS             15183..15860
                     /gene="narI"
                     /locus_tag="LGJ20_001319"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBK2385413.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16044..16175)
                     /locus_tag="LGJ20_001320"
     ncRNA           complement(16044..16175)
                     /ncRNA_class="other"
                     /locus_tag="LGJ20_001320"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16222..16306)
                     /locus_tag="LGJ20_001321"
     tRNA            complement(16222..16306)
                     /locus_tag="LGJ20_001321"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16270..16272),aa:Tyr,seq:gta)
     gene            complement(16466..17308)
                     /gene="purU"
                     /locus_tag="LGJ20_001322"
     CDS             complement(16466..17308)
                     /gene="purU"
                     /locus_tag="LGJ20_001322"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBK2385414.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17358..17816)
                     /locus_tag="LGJ20_001323"
     CDS             complement(17358..17816)
                     /locus_tag="LGJ20_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBK2385415.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            17929..18834
                     /gene="rssA"
                     /locus_tag="LGJ20_001324"
     CDS             17929..18834
                     /gene="rssA"
                     /locus_tag="LGJ20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBK2385416.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            18926..19939
                     /gene="rssB"
                     /locus_tag="LGJ20_001325"
     CDS             18926..19939
                     /gene="rssB"
                     /locus_tag="LGJ20_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBK2385417.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20141..21049
                     /gene="galU"
                     /locus_tag="LGJ20_001326"
     CDS             20141..21049
                     /gene="galU"
                     /locus_tag="LGJ20_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK2385418.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21193..21606)
                     /gene="hns"
                     /locus_tag="LGJ20_001327"
     CDS             complement(21193..21606)
                     /gene="hns"
                     /locus_tag="LGJ20_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBK2385419.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22211..22828
                     /gene="tdk"
                     /locus_tag="LGJ20_001328"
     CDS             22211..22828
                     /gene="tdk"
                     /locus_tag="LGJ20_001328"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK2385420.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYISVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23130..25805)
                     /gene="adhE"
                     /locus_tag="LGJ20_001329"
                     /gene_synonym="adhC"
     CDS             complement(23130..25805)
                     /gene="adhE"
                     /locus_tag="LGJ20_001329"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK2385421.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            26282..26929
                     /gene="ychE"
                     /locus_tag="LGJ20_001330"
     CDS             26282..26929
                     /gene="ychE"
                     /locus_tag="LGJ20_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBK2385422.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(27086..27382)
                     /locus_tag="LGJ20_001331"
     CDS             complement(27086..27382)
                     /locus_tag="LGJ20_001331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385423.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            27666..29297
                     /gene="oppA"
                     /locus_tag="LGJ20_001332"
     CDS             27666..29297
                     /gene="oppA"
                     /locus_tag="LGJ20_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBK2385424.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            29383..30303
                     /gene="oppB"
                     /locus_tag="LGJ20_001333"
     CDS             29383..30303
                     /gene="oppB"
                     /locus_tag="LGJ20_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBK2385425.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            30318..31226
                     /gene="oppC"
                     /locus_tag="LGJ20_001334"
     CDS             30318..31226
                     /gene="oppC"
                     /locus_tag="LGJ20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBK2385426.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            31238..32251
                     /gene="oppD"
                     /locus_tag="LGJ20_001335"
     CDS             31238..32251
                     /gene="oppD"
                     /locus_tag="LGJ20_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBK2385427.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            32248..33252
                     /gene="oppF"
                     /locus_tag="LGJ20_001336"
     CDS             32248..33252
                     /gene="oppF"
                     /locus_tag="LGJ20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBK2385428.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(33305..33634)
                     /locus_tag="LGJ20_001337"
     CDS             complement(33305..33634)
                     /locus_tag="LGJ20_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBK2385429.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(33669..35129)
                     /gene="cls"
                     /locus_tag="LGJ20_001338"
     CDS             complement(33669..35129)
                     /gene="cls"
                     /locus_tag="LGJ20_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK2385430.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            35272..35445
                     /locus_tag="LGJ20_001339"
     CDS             35272..35445
                     /locus_tag="LGJ20_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBK2385431.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(35500..36753)
                     /gene="kch"
                     /locus_tag="LGJ20_001340"
     CDS             complement(35500..36753)
                     /gene="kch"
                     /locus_tag="LGJ20_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBK2385432.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(37052..37348)
                     /locus_tag="LGJ20_001341"
     CDS             complement(37052..37348)
                     /locus_tag="LGJ20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBK2385433.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            37572..38291
                     /gene="tonB"
                     /locus_tag="LGJ20_001342"
     CDS             37572..38291
                     /gene="tonB"
                     /locus_tag="LGJ20_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBK2385434.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(38331..38729)
                     /gene="yciA"
                     /locus_tag="LGJ20_001343"
     CDS             complement(38331..38729)
                     /gene="yciA"
                     /locus_tag="LGJ20_001343"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBK2385435.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(38834..39373)
                     /locus_tag="LGJ20_001344"
     CDS             complement(38834..39373)
                     /locus_tag="LGJ20_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBK2385436.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(39403..40146)
                     /locus_tag="LGJ20_001345"
     CDS             complement(39403..40146)
                     /locus_tag="LGJ20_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBK2385437.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(40174..40263)
                     /locus_tag="LGJ20_001346"
                     /pseudo
     CDS             complement(40174..40263)
                     /locus_tag="LGJ20_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            40503..41141
                     /gene="ompW"
                     /locus_tag="LGJ20_001347"
     CDS             40503..41141
                     /gene="ompW"
                     /locus_tag="LGJ20_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBK2385438.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(41201..41707)
                     /locus_tag="LGJ20_001348"
     CDS             complement(41201..41707)
                     /locus_tag="LGJ20_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBK2385439.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(41753..42253)
                     /locus_tag="LGJ20_001349"
     CDS             complement(41753..42253)
                     /locus_tag="LGJ20_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBK2385440.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(42339..42518)
                     /locus_tag="LGJ20_001350"
     CDS             complement(42339..42518)
                     /locus_tag="LGJ20_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBK2385441.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(42899..43705)
                     /gene="trpA"
                     /locus_tag="LGJ20_001351"
     CDS             complement(42899..43705)
                     /gene="trpA"
                     /locus_tag="LGJ20_001351"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBK2385442.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(43705..44898)
                     /gene="trpB"
                     /locus_tag="LGJ20_001352"
     CDS             complement(43705..44898)
                     /gene="trpB"
                     /locus_tag="LGJ20_001352"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBK2385443.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(44910..46271)
                     /gene="trpCF"
                     /locus_tag="LGJ20_001353"
     CDS             complement(44910..46271)
                     /gene="trpCF"
                     /locus_tag="LGJ20_001353"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBK2385444.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPRASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(46272..47867)
                     /gene="trpD"
                     /locus_tag="LGJ20_001354"
     CDS             complement(46272..47867)
                     /gene="trpD"
                     /locus_tag="LGJ20_001354"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBK2385445.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(47867..49429)
                     /gene="trpE"
                     /locus_tag="LGJ20_001355"
     CDS             complement(47867..49429)
                     /gene="trpE"
                     /locus_tag="LGJ20_001355"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBK2385446.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARSVLR
                     AIATAHHAQETF"
     misc_feature    complement(49451..49544)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(49521..49565)
                     /gene="trpL"
                     /locus_tag="LGJ20_001356"
     CDS             complement(49521..49565)
                     /gene="trpL"
                     /locus_tag="LGJ20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBK2385447.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            49703..50584
                     /gene="yciV"
                     /locus_tag="LGJ20_001357"
     CDS             49703..50584
                     /gene="yciV"
                     /locus_tag="LGJ20_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBK2385448.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            50581..51201
                     /locus_tag="LGJ20_001358"
     CDS             50581..51201
                     /locus_tag="LGJ20_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK2385449.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            51302..52174
                     /gene="rluB"
                     /locus_tag="LGJ20_001359"
     CDS             51302..52174
                     /gene="rluB"
                     /locus_tag="LGJ20_001359"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBK2385450.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(52214..52804)
                     /gene="cobO"
                     /locus_tag="LGJ20_001360"
     CDS             complement(52214..52804)
                     /gene="cobO"
                     /locus_tag="LGJ20_001360"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBK2385451.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(52801..53559)
                     /locus_tag="LGJ20_001361"
     CDS             complement(52801..53559)
                     /locus_tag="LGJ20_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBK2385452.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            53779..54828
                     /gene="sohB"
                     /locus_tag="LGJ20_001362"
     CDS             53779..54828
                     /gene="sohB"
                     /locus_tag="LGJ20_001362"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBK2385453.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(54864..55115)
                     /locus_tag="LGJ20_001363"
     CDS             complement(54864..55115)
                     /locus_tag="LGJ20_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBK2385454.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            55495..58092
                     /gene="topA"
                     /locus_tag="LGJ20_001364"
     CDS             55495..58092
                     /gene="topA"
                     /locus_tag="LGJ20_001364"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK2385455.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            58302..59276
                     /gene="cysB"
                     /locus_tag="LGJ20_001365"
     CDS             58302..59276
                     /gene="cysB"
                     /locus_tag="LGJ20_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBK2385456.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            59571..59735
                     /locus_tag="LGJ20_001366"
     CDS             59571..59735
                     /locus_tag="LGJ20_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBK2385457.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            59738..59905
                     /locus_tag="LGJ20_001367"
     CDS             59738..59905
                     /locus_tag="LGJ20_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385458.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            60019..60114
                     /locus_tag="LGJ20_001368"
     CDS             60019..60114
                     /locus_tag="LGJ20_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385459.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            60278..>61144
                     /locus_tag="LGJ20_001369"
     CDS             60278..>61144
                     /locus_tag="LGJ20_001369"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBK2385460.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYG"
BASE COUNT        15376 a        14726 c        15648 g        15394 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagcgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcatcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattcct atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgggcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc tcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagctc cacaagagta
     2761 ccgatttcca ccccatcaat aagaggaagt gtgctaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catcaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacattca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt ttttcattat taccggaagt taccgacgtt
     3241 ttgagccgtt tcgttcctcg cattatcccg ttttatttac tcttgcttgt agcaggcggt
     3301 acagctaacg cacaatctac cttcgagcaa aaagcggcaa atccctttga taataacaat
     3361 gatggtctgc cggatttagg catggctccc gaaaatcatg atggggaaaa acactttgct
     3421 gaaattgtga aagatttcgg cgaaaccagt atgaatgata acgggctgga tactggcgag
     3481 caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc ttagtcaaca ggttaatcag
     3541 cacgtagagt cctggctatc actgtgggga aatgccagtg ttgacgtcaa agtggataac
     3601 gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc cgttacaaga taatgatcgt
     3661 tatctcacct ggagccagct tggtcttact cagcaggatg atgggttggt gagcaatgtg
     3721 ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg gttataacac tttttatgac
     3781 aacttgctgg acgaaaatct tcagcgagcg ggctttggtg ccgaagcgtg gggcgaatat
     3841 ttgcgactat cggcaaactt ttatcagccg tttgctgcat ggcatgaaca gacagccacg
     3901 caggaacaac ggatggcgcg cgggtatgac ctgacagccc ggatgcgcat gccgttctat
     3961 caacacctca ataccagtgt cagcgtagaa cagtattttg gtgatcgtgt tgatttgttt
     4021 aactctggta cgggttatca caatcccgtc gcgttgagtc tgggattaaa ttacacccct
     4081 gtgccattag tcactgtgac ggcccagcat aaacagggtg aaagtggcga gaatcaaaat
     4141 aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc tcaaaaaaca actttctgcg
     4201 ggtgaggttg ccgaaagtca gtcgttacgt ggtagtcgct atgacaatcc gcagcgaaat
     4261 aatctaccga ctcttgagta ccgacagcga aaaacgttaa cggtgtttct ggcgacaccg
     4321 ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat tacaaatccg cagtcgttac
     4381 ggtattcggc aactgatttg gcagggcgat acgcagatat taagtttgac gccgggcgca
     4441 caagccaaca gcgcggaggg ctggacgctg atcatgcctg actggcagaa cggggaaggg
     4501 gcaagcaatc actggcgatt gtcggtggtt gtggaagata accaggggca gcgtgtctcc
     4561 tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg cattgtcaaa cgacgaactg
     4621 cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc cataccgctg cttccacgcg
     4681 agacttgagc ttcattttct tcagcatgtg cttgacgtgc acttttactg tgctttcggt
     4741 gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc tgggcaatca gcttgagaat
     4801 atcgcgctcg cgtggggtta actggttaac atcgcgctca gtagtggcac ggttagcgcg
     4861 caagctggcg gccagaacag gcgttaatgc ttcgcttaat accatttcgc cagcagctgc
     4921 ctgatgcaat gctttcagca gatcttccgg ttccatatct tttaacagat agccatccgc
     4981 gccgcgtttc agtgcggtga ccacatcttc ttcatggtta gagacgctga ataccacaat
     5041 gcgccctgag agggactttt cgcgcagttt atccagcgtt tccagaccgt tcatgccggg
     5101 catattgaga tctaacagga tcagatcggg atcaagagat tccgccagtt caataccctg
     5161 ttcgccatta ctcgcttcgc caaccacggt gatagctggt gccatactga taagctgttt
     5221 tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg atagtagccg gttcctgatt
     5281 actcatgggt atctccttgg acgtctgtga aagttttttc gggaataaag gtgaccacca
     5341 cttcggtgcc acctgattca cgacggcgga cgcggcaatc gcctcgtaaa ctttgcgcac
     5401 ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat ggcattttca ggcacgccgc
     5461 agccgttatc ctgtacggtc agtttgacct gattatcgtt ttgcgccacc gtcaccacga
     5521 cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc ctcacgggca atttgcaaca
     5581 agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa ttgataatcc agcttcaccg
     5641 ggaagccaaa tttggcgctg tactcttcgc aactcgcctc cagcgccgga cgtaatccag
     5701 gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg caactgcgcc caggatgcat
     5761 tcagttcgtt acggatctga cttaacagtt cgcggctgct ttctggcagc gcatcgccct
     5821 gcatctgtaa acaactcacc tgcatcttca tacaagagag agattgggca atagaatcat
     5881 gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat caactgttgc tgacgttcct
     5941 gatggcgatc caacgccagc gtggcggtga gttgttcaac cagggtatcc accagttgtt
     6001 gttgatcatg gctaagatga cgcccctgcg gcagggtcgc cagcaaaata ccgtactgcg
     6061 tatgagagtc agccagccgc cacttcaggg ttgtgccgcg atcgccaacg ggtaatacgc
     6121 cgcgcgggca gagctggcag cctttatcat cacaagtcat atctggttgg caggtaaact
     6181 cctgatgatt ctcttcatca tcagtatcat aaacccgcag ttcgatatca cgtagcaggg
     6241 ttaaattctg taagccgttg agtacaggtg acaggcgttc acacagcggg gcttgggaat
     6301 gcaaacggcg gttagcctgc cataaaaaag agaggatctg atttttatgc tccagcccgg
     6361 cggttttctc ctgaacccgc tgctcaagta cggcataact ttcggccagt tctgcagaca
     6421 tattgttcaa cgcagttcca agcatcgcca tttcgttgcg cccgctgatg tttgcgcgtt
     6481 gggtaaaatc gcgatgactg acggcactcg ccattgccag cagttgccgc cacggttgta
     6541 gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa aagtgccata aataccgcca
     6601 ttacccgatg gaccagtacc actgtctcga tgcgcatttc cgtggtgcgg tcaaaaccag
     6661 ataccagttg atcaagcccg gcaacaaact ggctgacatc cgctgacacc gtttctcggt
     6721 tttgtgcacg catcaacgca gggatcagtt cattacgcca gtaatcttgt aaaccctgta
     6781 attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa ctcggcgcta aatgccgttt
     6841 gttccatctc tttaattaag ggcttgtctt tctcgcttaa tggcactgct gccaacagac
     6901 ggtaactttg catgcgcagc gatcccgctt tgttgatcgc atgggcgctg ccctgaacgc
     6961 cttgcaccag ccagccagaa accgccatcc ctgccagtcc aatagcagta gaaagcaaca
     7021 caataagcgc aacctgatta accagggtga gcggagagag acaacgttta agcatgtaaa
     7081 cctcttcctt caggctttaa atgagcaata accttaatga atgtgacgat acattctgga
     7141 atggcagtat tctcggctat tggctgaagt atacccatac ccggaaagag ttactcctta
     7201 tttgccgtgt ggttagtcgc tttacatcgg taagggtagg gattctacag caccgtgaaa
     7261 aatctcataa tttttatgaa atcactgtac tcactatggg taatgataaa tatcaatgat
     7321 agataaagtt atcttatcgt ttgatttaca tcaaattgcc tttagctaca gacactaagg
     7381 tggcagacat cgaaacgagt atcagaggtg tctatgagtc actcatccgc ccccgaaagg
     7441 gctactggag ctgtcattac agattggcga ccggaagatc ctgcgttctg gcaacaacgc
     7501 ggtcaacgta ttgccagccg taacctgtgg atttccgttc cctgtctgct gctggcgttt
     7561 tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac cgaaagtcgg ttttaatttt
     7621 acgaccgatc agctatttat gttgactgcg ctgccttcgg tttctggcgc gttattacgt
     7681 gttccatact cctttatggt tcctatcttc ggtggtcgtc gctggacggc gttcagcacc
     7741 ggtattctga ttattccttg cgtctggctg ggttttgccg tgcaggatac ctccacgcct
     7801 tatagcgtct tcatcatcat ctctctgcta tgcggctttg ctggcgcgaa cttcgcatcc
     7861 agtatggcaa acatcagctt cttctttccg aaacagaagc agggtggcgc gctgggtctg
     7921 aatggtggtc tgggaaacat gggcgtcagc gtcatgcagt tggttgctcc gctggtggta
     7981 tcactgtcga ttttcgcagt atttggtagc cagggcgtca aacagccgga tgggactgag
     8041 ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc ttgccatctt caccattgca
     8101 gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct ccatcaagga gcagttgccg
     8161 gtactcaaac ggggtcatct gtggattatg agcctgctgt atctggcaac cttcggctcc
     8221 tttatcggct tctccgcggg ctttgcaatg ctgtcaaaaa cgcagttccc ggatgttcag
     8281 attctgcaat acgctttctt cgggccgttt attggtgcgc tggcgcgttc tgcaggtggt
     8341 gcattatctg accgtctggg cggaactcgt gtcacgctgg tgaactttat tctgatggcg
     8401 attttcagcg gcctgctgtt cctgacctta cctactgacg ggcagggcgg aagcttcatg
     8461 gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg ggctgggtag tggttccact
     8521 ttccagatga tttcagtgat ctttcgtaaa ctgacaatgg atcgcgtgaa agcagaaggg
     8581 ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca cggcggcggc gttgggtttc
     8641 atctctgcga ttggcgcgat aggtggcttc tttatcccga aagcgttcgg tagctcgctg
     8701 gcattaacgg gttcgccagt tggcgcaatg aaagtatttt tgattttcta tatcgcctgc
     8761 gtagtgatta cctgggcggt atatggtcgg cattctaaaa aataaatctt aatatttgat
     8821 tatcctttgc gcggcatgat gtcgcgcttt ttttatgcgt catttagtta caacatactc
     8881 atattatatg gcttatttcg ccggatttca ttaagagcca ttaatatgtt acccatgggg
     8941 aatactcctt aatacccatc tgcataaaaa tcttaatagt ttaaataact acaggtataa
     9001 aacgtcttaa tttacagtct gttatgtggt gtctgttaat tatcctaaag gggtatctta
     9061 ggaatttact ttatttttca tccccatcac tcttgatcgt tatcaattcc cacgctgttt
     9121 cagagcgtta ccttgccctt aaacattagc aatgtcgatt tatcagaggg ccgacaggct
     9181 cccacaggag aaaaccgatg agtaaattcc tggaccggtt tcgctacttc aaacagaagg
     9241 gtgaaacctt tgccgatggg catggccagc ttctcaatac caaccgtgac tgggaggatg
     9301 gatatcgcca gcgttggcag catgacaaaa tcgtccgctc tacccacggg gtaaactgca
     9361 ccggctcctg cagctggaaa atctacgtca aaaacggtct ggtcacctgg gaaacccagc
     9421 agactgacta tccgcgtacc cgtccggatc tgccaaacca tgaacctcgc ggctgcccgc
     9481 gcggtgccag ctactcctgg tatctttaca gtgccaaccg cctgaaatac ccgatgatgc
     9541 gcaaacgcct gatgaaaatg tggcgtgaag cgaaggcgct gcatagcgat ccggttgagg
     9601 catgggcttc tatcattgaa gacgccgata aagcgaaaag ctttaagcag gcgcgtggac
     9661 gcggtggatt tgttcgttct tcctggcagg aggtgaacga actgatcgcc gcatctaacg
     9721 tttacaccat caaaaactac ggcccggacc gtgttgctgg tttctcgcca attccggcaa
     9781 tgtcgatggt ttcttacgca tcgggtgcac gctatctctc gctgattggc ggtacttgct
     9841 taagcttcta cgactggtac tgcgacttgc ctcctgcgtc tccgcaaacc tggggcgagc
     9901 aaactgacgt accggaatct gctgactggt acaactccag ctacatcatc gcctgggggt
     9961 caaacgtgcc gcagacgcgt accccggatg ctcacttctt tactgaagtg cgttacaaag
    10021 ggaccaaaac tgttgccgtc acaccagact acgctgaaat cgccaaactg tgcgatctgt
    10081 ggctggcacc gaaacagggc accgatgcgg caatggcgct ggcgatgggc cacgtaatgc
    10141 tgcgtgaatt ccacctcgac aacccaagcc agtatttcac cgactatgtg cgtcgctaca
    10201 ccgacatgcc gatgctggtg atgctggaag aacgcgacgg ttactacgct gcaggtcgta
    10261 tgctgcgcgc tgctgatctg gttgatgcgc tgggccagga aaacaatccg gaatggaaaa
    10321 ctgtcgcctt taataccaat ggcgaaatgg ttgcgccgaa cggttctatt ggcttccgct
    10381 ggggcgagaa gggcaaatgg aatcttgagc agcgcgacgg caaaactggc gaagaaaccg
    10441 agctgcaact gagcctgctg ggtagccagg atgagatcgc tgaggtaggc ttcccgtact
    10501 ttggtggcga cggcacggaa cacttcaaca aagtggaact ggaaaacgtg ctgctgcaca
    10561 aactgccggt gaaacgcctg caactggctg atggcagcac cgccctggtg accaccgttt
    10621 atgatctgac gctggcaaac tacggtctgg aacgtggcct gaacgacgtt aactgtgcaa
    10681 ccagctatga cgatgtgaaa gcttataccc cggcctgggc cgagcagatt accggcgttt
    10741 ctcgcagcca gattattcgc atcgcccgtg aatttgccga taacgctgat aaaacgcacg
    10801 gtcgttcgat gattatcgtc ggtgcggggc tgaaccactg gtatcacctc gatatgaact
    10861 atcgtggtct gatcaacatg ctgattttct gcggctgtgt cggtcagagc gggggcggct
    10921 gggcgcacta tgtaggtcag gaaaaactgc gtccgcaaac cggctggcag ccgctggcgt
    10981 ttgcccttga ctggcagcgt ccggcgcgtc acatgaacag cacttcttat ttctataacc
    11041 actccagcca gtggcgttat gaaaccgtca cggcggaaga gttgctgtca ccgatggcgg
    11101 acaaatcccg ctataccgga cacttgatcg actttaacgt ccgtgcggaa cgcatgggct
    11161 ggctgccgtc tgcaccgcag ttaggcacta acccgctgac tatcgctggc gaagcggaaa
    11221 aagccgggat gaatccggtg gactatacgg tgaaatccct gaaagagggt tccatccgtt
    11281 ttgcggcaga acaaccagaa aacggtaaaa accacccgcg caacctgttc atctggcgtt
    11341 ctaacctgct cggttcttcc ggtaaaggtc atgagtttat gctcaagtac ctgctgggga
    11401 cggagcacgg tatccagggt aaagatctgg ggcaacaggg cggcgtgaag ccggaagaag
    11461 tggactggca ggacaatggt ctggaaggca agctggatct ggtggttacg ctggacttcc
    11521 gtctgtcgag cacctgtctc tattccgaca tcattttgcc gacggcgacc tggtacgaaa
    11581 aagacgacat gaatacttcg gatatgcatc cgtttattca cccgctgtct gcggcggtcg
    11641 atccggcctg ggaagcgaaa agcgactggg aaatctacaa agccatcgcg aagaaattct
    11701 ccgaagtgtg cgtcggccat ctgggtaaag aaaccgacat cgtcacgctg cctatccagc
    11761 atgactctgc cgctgaactg gcgcagccgc tggatgtgaa agactggaaa aaaggcgagt
    11821 gcgacctgat cccaggtaaa accgcgccac acattatggt cgtagagcgt gattatccgg
    11881 cgacttacga acgctttacc tctatcggcc cgctgatgga gaaaatcggt aatggcggta
    11941 aagggattgc ctggaacacc cagagcgaga tggatctgct gcgtaagctc aactacacca
    12001 aagcggaagg tccggcgaaa ggccagccga tgctgaacac cgcaattgat gcggcagaga
    12061 tgatcctgac actggcaccg gaaaccaacg gtcaggtagc cgtgaaagcc tgggctgccc
    12121 tgagcgaatt taccggtcgt gaccatacgc atctggcgct gaataaagaa gacgagaaga
    12181 tccgcttccg cgatattcag gcacagccgc gcaaaattat ctccagcccg acctggtctg
    12241 gtctggaaga tgaacacgtt tcttacaacg ccggttacac caacgttcac gagctgatcc
    12301 catggcgtac gctcactggt cgtcagcaac tgtatcagga tcaccagtgg atgcgtgatt
    12361 tcggtgaaag cctgctggtt tatcgtccgc cgatcgacac ccgttcggtg aaagaagtga
    12421 taggccagaa atccaacggc aacccggaaa aagcgctcaa cttcctgacg ccgcaccaga
    12481 agtggggtat ccactccacc tacagcgaca acctgctgat gctgacttta ggtcgcggtg
    12541 gtccggtggt ctggttgagt gaagccgatg ccaaagatct gggtatcgcc gataacgact
    12601 ggattgaagt attcaacagc aacggtgctc tgactgcccg tgcggttgtc agccagcgtg
    12661 ttccggcagg gatgaccatg atgtaccacg cgcaggaacg tatcgttaac ctgcctggtt
    12721 cggaaattac ccaacagcgt ggtggtatcc ataactcggt cacccgtatc acgccgaaac
    12781 cgacgcatat gatcggcggc tatgcccatc tggcatacgg ctttaactac tacggcaccg
    12841 taggttctaa ccgcgatgag tttgttgtag tgcgtaagat gaagaacatt gactggttag
    12901 atggcgaagg caatgaccag gtacaggaga gcgtaaaatg aaaattcgtt cacaaatcgg
    12961 catggtgctg aatctcgata agtgcatcgg ctgccacacc tgttcagtta cctgtaaaaa
    13021 cgtctggacc agccgtgaag gcgtggaata cgcgtggttc aacaacgtgg aaaccaagcc
    13081 gggccagggc ttcccgactg actgggaaaa ccaggaaaaa tacaaaggcg gctggatccg
    13141 taaaatcaac ggcaaactgc agccgcgcat gggtaaccgt gccatgctgc tgggtaaaat
    13201 cttcgctaac ccgcatctgc cggggatcga cgattattac gagccgttcg attttgacta
    13261 tcagaacctg cataccgcgc cggaaggcag caaatcgcag ccgattgccc gtccgcgttc
    13321 gctgattacc ggcgaacgga tggcgaaaat cgaaaaaggg ccgaactggg aagatgacct
    13381 gggcggtgag tttgacaagc tggcgaaaga caagaacttc gacaacatcc agaaggcgat
    13441 gtatagccag ttcgaaaaca cctttatgat gtatttgccg cgcctgtgcg aacactgcct
    13501 gaacccggca tgtgtggcga cctgcccgag cggtgcgatt tacaagcgtg aagaagatgg
    13561 catcgtcctg atcgaccagg ataaatgccg tggctggcgt atgtgcatca ccggatgccc
    13621 gtacaaaaaa atctacttca actggaagag cggtaagtct gagaagtgca tcttctgcta
    13681 tccgcgtatt gaagcgggtc agccgaccgt gtgctcagaa acctgtgtcg gtcgtatccg
    13741 ttatcttggc gtgctgctgt acgatgccga cgcgattgaa cgtgcagcca gcaccgagaa
    13801 cgagaaagat ctttaccagc gtcagctgga ggtgttcctc gatccgaacg atccgaaagt
    13861 catcgagcag gcgattaaag acggtattcc gctgagcgtt attgaagccg cacagcagtc
    13921 gccggtttat aaaatggcaa tggaatggaa actggcgctg ccgctgcatc cagaatatcg
    13981 cacactgccg atggtctggt acgtgccgcc tctgtctccg attcagtctg cggcagacgc
    14041 gggtgagctg ggtagcaacg gcattctgcc agacgtcgaa agcttgcgta ttccggtaca
    14101 gtatctggcg aatctgctga ccgccggtga taccaaaccg gtactgcgcg cactgaaacg
    14161 tatgctggcg atgcgtcatt acaaacgtgc tgaaaccgtt gacggtaaag ttgatacccg
    14221 tgcgctggaa gaggtcggtc tgaccgaagc ccaggcacag gagatgtacc gttatctggc
    14281 gattgctaac tacgaagatc gctttgtggt gccgagtagt catcgtgaac tggcacggga
    14341 agccttcccg gagaaaaatg gctgcggctt tacctttggt gatggctgcc acggttcaga
    14401 taccaaattc aatctgttca acagccgtcg tatcgatgcc atcgatgtga ccagcaaaac
    14461 ggagccgcat ccatgatcga actcgtgatt gtatcgcgtc tccttgaata tccggatgct
    14521 gccttatggc agcatcaaca agagatgttt gaggcgattg ctgcgtcgaa aaatctgtcg
    14581 aaagaagatg cccatgcgct gggcattttc ctgcgcgatt taacggcgat ggatccgctc
    14641 gatgcccagg cccagtacag cgaactgttc gaccgtggcc gcgccacgtc actgttgcta
    14701 tttgaacatg ttcacggtga atcccgtgac cgcggccagg cgatggtgga cctgctggcg
    14761 cagtacgagc agcacggctt gcagttaaac agccgcgaat taccggacca tctgccgctg
    14821 tatctggagt acctgtcgca gctgccgcaa agcgaagccg tggaaggttt gaaagatatc
    14881 gcgccgattc tggcattgct gagcgcgcgt ctgcaacagc gtgaaagccg ttatgccgtg
    14941 atgtttgatc tgctgctgaa attggccaat accgctatcg acagcgacaa agtggcggaa
    15001 aaaattgccg acgaagcgcg cgatgatacg ccgcaggcgc tggatgctgt ctgggaagaa
    15061 gagcaggtta aattctttgc tgacaaaggc tgcggtgatt cagcaatcac tgctcatcag
    15121 cgtcgctttg ccggtgccgt cgcgccgcaa tatctgaata tcaccaccgg aggacagcac
    15181 taatgcaatt cctgaatatg ttcttctttg atatctaccc gtacatagcc ggggcggtct
    15241 tcctgattgg tagctggctg cgttatgact acgggcagta cacctggcgc gcggcgtcca
    15301 gccagatgct ggatcgcaaa gggatgaacc tggcgtcgaa cctgttccat atcggtattc
    15361 tggggatttt tgttggtcac ttcttcggta tgctgacgcc gcactggatg tatgaagcct
    15421 ggctgccgat tgaagtgaaa cagaaaatgg cgatgtttgc tggtggtgcc agcggcgtgc
    15481 tgtgtctgat tggcggcgtg ctgttgctga agcgtcgtct gttcagccca cgcgtgcgtg
    15541 caaccactac cggagcggat atcctgatcc tgtcgctgct cgttatccag tgcgcgctgg
    15601 gcctgttgac cattccgttc tccgctcagc atatggacgg tagcgagatg atgaaactgg
    15661 ttggctgggc gcagtcggtg gtgaccttcc acggtggcgc ttctcaacac ctcgatggtg
    15721 tggcgtttat cttccgtctg cacctggtgc tggggatgac gttattcctg ctgttcccgt
    15781 tctcgcgtct ggtacacatc tggagcgtac cggtggagta tctgacacgt aagtaccagc
    15841 tggtgcgcgc tcgtcactaa gcgaatttta gttcacatag accctgcttc ggcggggttt
    15901 ttttatgggc acggtgcggg gtgagttgtc ggatgcgctt cgcttatccg acctacaggg
    15961 gaggatattg taggcccggt aaacgtggtg ccatcgggca aaattagctc ggagtaacag
    16021 gttttgatta tttaaggaag tgatggtggt gggggaagga ttactcaacg ctgcgcgctt
    16081 cgcccttcgg gtcgttgcct gcggcaacgc tctctcgctg gcgctcgagt cgaaccttgg
    16141 tcgaggcttc tcatccttcc ccgcatgggc agaatattta atcgcggatt cgtttgagag
    16201 ttcacggact tttgaaagtg atggtggtgg gggaaggatt cgaaccttcg aagtcgatga
    16261 cggcagattt acagtctgct ccctttggcc gctcgggaac cccaccacgg ggtaatgctt
    16321 tttactggcc tgctccctta tcgggaagcg gggcgcatca tatcaaatga cgcgccgctg
    16381 taaagtgtta cgttgagaaa aatgaactgg ttgcgtaatt ttcatccgta acggattaaa
    16441 ggtaaccagt tatttttgct ggcgattaaa gaataatcgt ccgattaccg taaacaaaga
    16501 cgcgctgtgc cagtactttg tatagcgcac gacttaagac gtttttctcg acgtcacgac
    16561 ctgcgcgcat catatcttca gctgtgtagg tatgatcgac atgaataacg tcctgcatga
    16621 tgattgggcc ttcgtccaga ttgtcattca catagtgagc ggttgcgcca ataatcttca
    16681 caccacgttc ataggcctgg tgataaggac gtgcgccgat aaacgcaggc aggaatgaat
    16741 ggtgaatatt gatgatttta ttcgggaagc gtgccacaaa ttccggcgtt aatacccgca
    16801 tatacttcgc cagcaccaca tagtcaggtt gataagcatc aatggcgtcc gccatctttt
    16861 gatcgtgctc gttgcggctt aacccttcat ggcttaccag ctcaaacgga atatcaaaac
    16921 gctcaaccag agaacgtaaa gtatcgtggt taccaataac tgccgcgatt tcgacatcca
    16981 ggccgccgta attggctttc atcaacaaat cgccaaggca atgcgcttct ttagtgacca
    17041 gaatcactat ccggcgacga ccggcaggat tcagctcacg cacggagcct tctggcaatg
    17101 cgctatcgag atccgccagc agggtggaat cattaaaaat cccttccagt tccgtgcgca
    17161 taaaaaagcg cccggtacgg tgatcaacaa attcattgtt ctgtacgata tttaactcgt
    17221 gcttgtagca aatattggta atacgtgcga tcagaccttt ttggtccgga caaatagtac
    17281 gcagaacttt acgttggagt gaatgcattg ctggaaaaac cttgttgaga gtgtttgcta
    17341 aaccgtaccg tcaaccatta ttggccgcag cactttttaa attttttacc tgaaccacaa
    17401 gggcagggat cgttgcgacc aaactgcgga cgtgtaccgt caatatagta ccattgaccg
    17461 ttttctttca aaaatcgcga acgttcaatt attgcacctg ttttaccacc ttcggtaaat
    17521 cgagcaacaa aactgacaaa tccgatattg tcggcgtcct gccaacaatg ttcaaatacg
    17581 gtcaatccca accattcagt atgcgcaaat ccggccatca attcggcacg taacgccgct
    17641 gctccacaag agggatgcca ggtctttatt aaataatctg cgtcttgcat cacaaaagcg
    17701 cagtaacgcg aacgcatgag atgttctgga tcaggtgcaa ccttttcacc agacacataa
    17761 gggtggcaac ataggctata ctcgacagca ctaccacagg gacaaagctg agacacaaat
    17821 aatctccctg gaaacaataa cggcgtatta accgcctgag tagcactatg ttaaccgagc
    17881 agtagcgatg tggctacgat tgcattccag gggaatcttg cgggaataat gagaaagata
    17941 aaaatagggc tggcgctggg atctggcgcg gcgagaggtt ggtcgcatat tggcgttatt
    18001 aatgcgctaa aaaaagtggg tattgaaatt gatatcgttg caggatgttc aattggttcg
    18061 ctggtgggcg ctgcctatgc atgcgatcga ttatctgcgc tggaagattg ggtgacctct
    18121 ttcagttatt gggatgtttt acgcctgatg gatctctcct ggcagcgcgg tgggttactg
    18181 cgcggcgagc gtgtcttcaa tcaataccgc gaaataatgc cggaaacaga gatcgaaaat
    18241 tgttcccgtc gctttgcggc tgttgccacc aatttaagta cgggacgtga attatggttt
    18301 actgaaggcg atctccatct tgctattcgc gcatcatgca gtattccagg actcatggca
    18361 cctgttgcac ataacggcta ctggctggtt gatggagcag tcgttaaccc aattcctatt
    18421 tccctcacgc gtgcattggg ggctgatatt gtgatagcgg ttgacctgca gcacgatgct
    18481 catttgatgc aacaagattt gctctccttt aatgtcagtg aagaaaatag cgagaatggt
    18541 gattctctgc cgtggcatgc gcgtctgaaa gaaaggttag gcagcataac gacacgtcgg
    18601 gcggtgacag cgccaacggc aacagagatt atgaccactt ctatccaggt gctggagaac
    18661 cgccttaaaa ggaaccgcat ggcaggtgat ccgcccgata ttctgattca acctgtttgc
    18721 ccgcaaatat ctacgcttga tttccatcgc gcgcacgctg ccattgcggc cggacagctg
    18781 gcagtggaaa agaaaatgga cgaacttttg ccgttggtac gcaccaacat ttgaccagaa
    18841 tttttatcta cacttaagtt aattctgaca ggcgcaggtg gcaatagcat gccactattg
    18901 agtaaagcca gtcaggggag agaacatgac gcagccattg gtcggaaaac agattctcat
    18961 tgttgaagat gagccggtat ttcgctcgct tctggattcg tggttttcct cattgggagc
    19021 gacaacggta ctggcggctg atggggtgga tgcccttgag ttgctgggag gtttcactcc
    19081 agacctgatg atatgtgata tcgcgatgcc acgaatgaac gggcttaaac tgctggaaca
    19141 tatacgcaac agaggtgacc agacaccggt tctggtgata tctgccaccg aaaatatggc
    19201 agatattgcc aaagcgttac gtctgggcgt tgaagatgtt ttgctgaaac cagttaaaga
    19261 tctgaatcgc ttgcgcgaga tggtttttgc ctgtctctat cccagcatgt ttaattcgcg
    19321 cgttgaggaa gaggaaaggc tttttcgcga ctgggatgca atggttgata accctgccgc
    19381 agcggcgaaa ttattacagg aactacaacc gccggttcag caggtgattt cccattgccg
    19441 ggttaattat cgtcaattgg ttgccgcgga caaacccggc ctggtgcttg atattgccgc
    19501 actttcggaa aacgatctgg cattttattg ccttgatgtc acccgagctg gacataatgg
    19561 cgtacttgct gccttgttat tacgcgcatt gtttaacgga ttattacagg aacagcttgc
    19621 acaccaaaat caacggttgc cagagttggg cgcgttattg aagcaggtaa accatttact
    19681 tcgtcaggcc aatctgccgg ggcagtttcc gctattagtt ggctattatc atcgcgaact
    19741 gaaaaatctc attctggttt ctgcgggtct gaatgcgacg ttaaataccg gcgaacacca
    19801 ggtgcaaatc agtaatggtg ttccgttagg cactttaggt aacgcttatt tgaatcaatt
    19861 gagccagcga tgcgatgcct ggcaatgcca aatatgggga accggtggtc gactgcgctt
    19921 gatgttgtct gcagaatgag caaacgataa cgcgggctaa atttgcatta cctgctaatg
    19981 tcggctggtg gtactatcgt cgccattcgt ataagtaatt gtcttaatta tgctaactcg
    20041 cctccttttc agaacttagc cccttcgggg tgctgatata ctgggatgcg atacagaaat
    20101 atgaacacgt tcaaaacacg aacagtccag gagaatttaa atggctgcca ttaatacgaa
    20161 agtcaaaaaa gccgttatcc ccgttgcggg attaggaacc aggatgttgc cggcgacgaa
    20221 agccatcccg aaagagatgc tgccacttgt cgataagcca ttaattcaat acgtcgtgaa
    20281 tgaatgtatt gcggctggca ttactgaaat tgtgctggtt acacactcat ctaaaaactc
    20341 tattgaaaac cactttgata ccagttttga actggaagca atgctggaaa aacgtgtaaa
    20401 acgtcaactg cttgatgaag tgcagtctat ttgtccgccg cacgtaacta ttatgcaagt
    20461 tcgtcagggt ctggcgaaag gcctgggaca cgcggtattg tgtgctcacc cggtagtggg
    20521 tgatgaaccg gtagctgtta ttttgcctga tgttattctg gatgaatatg aatccgattt
    20581 gtcacaggat aacctggcag agatgatccg ccgctttgat gaaacgggtc atagccagat
    20641 catggttgaa ccggttgctg atgtgaccgc atatggcgtt gtggattgca aaggcgttga
    20701 attagcgccg ggtgaaagcg taccgatggt tggtgtggta gaaaaaccga aagcggatgt
    20761 tgcgccgtct aatctcgcta ttgtgggtcg ttacgtactt agcgcggata tttggccgtt
    20821 gctggcaaaa acccctccgg gagctggtga tgaaattcag ctcaccgacg caattgatat
    20881 gctgatcgaa aaagaaacgg tggaagccta tcatatgaaa gggaagagcc atgactgcgg
    20941 taataaatta ggttacatgc aggccttcgt tgaatacggt attcgtcata acacccttgg
    21001 cacggaattt aaagcctggc ttgaagaaga gatgggcatt aagaagtaac atccgtatcg
    21061 gtgttatcca cgaaacggcg ttgagcaatc gacgccgttt ttttatagct tattcttatt
    21121 aaattgtctt aaaccggaca ataaaaaatc ccgccgctgg cgggatttta agcaagtgca
    21181 atctacaaaa gattattgct tgatcaggaa atcgtcgagg gatttacctt gctcatccat
    21241 tgcttttttg attacagctg gagtacggcc ttggccagtc caggttttag tttcgccgtt
    21301 ttcgtcaacg tagctatatt ttgccggacg ctgagcacgt ttagctttgg tgccagattt
    21361 aacggcagca aggctattca gcagttcgtt cgggtcaata ccgtcagcga tcagcatttc
    21421 gcgatattgc tgcagtttac gagtgcgctc ttcaacttca gcagcagccg cgctttcttc
    21481 ttcgcgacgt tcgttaacaa caacttctaa tttttccagc atttcttcca gcgtttcaag
    21541 tgtacattct cttgcctgcg cacgaagagt acggatgttg ttcagaattt taagtgcttc
    21601 gctcattgta gtaatctcaa acttatattg gggtggtttg ttgaggtaat aatagagcct
    21661 taaattcagt tgtgcaatag ccaggaatgt aaggaattca aaattgttct ttattttgtg
    21721 ccgccaataa atatcttttc ataaaattag ccagaaaaga cgcggcatat agccctattt
    21781 acaccgatga tttcgcagca cgtgaggtta aaacttcctg attcatgtca cattttatgg
    21841 ggagattatc gtaggctgac gacctttcag tcttctgtat tagttgtgtt tacgagaatt
    21901 ccctattaag cgaatgatga aaagtagaac agtcgcaata agagcatgga cttagtattg
    21961 cactatctcc tggaggtcaa cagagggcta ttacttgcgc aacaggttaa agattgtgaa
    22021 tagttaccag cagtcattta cccgcttata acaagcgagg cagttgtaat gatagctcag
    22081 aaggattatg caaggcttcg taagggagaa cgcatatacc cacttctgtg catactgttg
    22141 agctgaaaaa ctgacgaatt atgataaact ccagccaact ttatttcata tcattgaggg
    22201 cctgtggctg atggcacagc tatatttcta ctattccgca atgaatgcgg gtaagtctac
    22261 agcattgttg caatcttcat acaattacca ggaacgcggc atgcgcactg tcgtatatac
    22321 ggcagaaatt gatgatcgct ttggtgccgg gaaagtcagt tcgcgtatag gtttgtcatc
    22381 gcctgcaaaa ttatttaacc aaaattcatc attatttgat gagattcgtg cggaacatga
    22441 acagcaggca attcattgcg tactggttga tgaatgccag tttttaacca gacaacaagt
    22501 atatgaatta tcggaggttg tcgatcaact cgatataccc gtactttgtt atggtttacg
    22561 taccgatttt cgaggtgaat tatttattgg cagccaatac ttactggcat ggtccgacaa
    22621 actggttgaa ttaaaaacca tctgtttttg tggccgtaaa gcaagcatgg tgctgcgtct
    22681 tgatcaagca ggcagacctt ataacgaagg tgagcaggtg gtaattggtg gtaatgaacg
    22741 atacatttct gtatgccgta aacactataa agaggcgtta caagtcggct cattaacggc
    22801 tattcaggaa aggcatcgcc acgattaata agaatttctt tactgacagg gtgagcaggg
    22861 cacttttatc ctgtcagttc gttttacgca cttcttccgg gctatatacc cttctcggca
    22921 gttttttaac gccgctatac gcctcacagg gctcttaagc accgacgttg acttgtgacc
    22981 tgtaaagtac aatatccctg tgtttaggcg ttatacatcg tcgcaaatat gatgaaggct
    23041 aatgctgtcg gtttatggaa aagttgcttt gggtaaacaa aaaaacggcc ccagaagggg
    23101 ccgtttatat tgccagacag cgctactgat taagcggatt ttttcgcttt tttctcagct
    23161 ttagccggag cggcttcttt tttcgctgca gtttcacctt ctacataatc acgaccgtag
    23221 taggtatcca gcaggatctg tttcagctcg gagatcagcg ggtaacgcgg gttagcgccg
    23281 gtgcactggt catcgaacgc atcttcagac agtttatcca cgttcgccag gaagtctgct
    23341 tcctgaacgc cagcttcacg gatagatttc ggaataccca gttcagcttt cagcgtttcc
    23401 agccatgcca gcagtttctc gatcttagca gcagtacggt cgcccggtgc gctcagaccc
    23461 aggtggtcgg caatttcagc ataacgacgg cgagcctgcg gacggtcata ctggctgaat
    23521 gcagtctgct tggtcgggtt gtcgttcgca ttgtagcgaa taacgttaca aatcagcagg
    23581 gcgtttgcca gaccgtgcgg aatatggaac tgggaaccca gtttgtgcgc cattgagtga
    23641 catacaccca ggaaggcgtt cgcaaacgcg atacccgcga tagtcgctgc actgtgaaca
    23701 cgttcacgcg ctaccggatt tttagaccct tcgtggtagg acgctggcag atattctttc
    23761 agcagtttca gtgcctgcag agcctgacca tcagagaact cagatgccag tacagaaaca
    23821 taagcttcca tggcgtgagt tactgcgtcc agaccaccga aagcacacag ggacttcggc
    23881 atgtccataa ccaggttggc gtcgacaatc gccatatccg gagtcagcgc atagtctgcc
    23941 agcggatatt tctgaccagt agcgtcgtca gttacaaccg caaacggagt gacttcagaa
    24001 cctgtaccag aagtggtggt gacagcgatc attttcgctt tcacgcccat tttcgggaac
    24061 ttgtagatac gtttacggat atccataaag cgcagcgcca gctcttcgaa gtgagtttcc
    24121 ggatgttcgt acataaccca catgatcttc gcggcgtcca tcggggaacc accacccagc
    24181 gcgataatca cgtctggttt gaaggagttt gccagttctg cacctttacg aacgatgctc
    24241 agggtcgggt ccgcttctac ttcgaagaag acttcagttt caacgcctgc tgctttcagt
    24301 acggaagtga tctgatcagc ataaccattg ttgaacagga agcggtcagt cacgatgagc
    24361 gcacgtttgt ggccatcagt aatcacttca tccagcgcga ttggcaggga gccacggcgg
    24421 aagtagatag atttcggaag tttgtgccac aacatgtttt cagctcgctt agcaacggtt
    24481 ttcttgttga tcaggtgttt cggaccaacg ttttcagaga tggagttacc accccaagaa
    24541 ccacaaccca gagtcaggga aggtgcgagt ttgaagttat acaggtcacc gataccaccc
    24601 tgagacgctg gggtgttaat caggatacga gccgttttca ttttctgacc gaagtaagaa
    24661 acgcgagccg gttggttatc ctggtcagtg tacaggcaag aggtatgacc gataccgccc
    24721 atagcaacca gtttctctgc tttttctacc gcgtcttcga aatctttagc gcggtacatt
    24781 gccagagtcg gggacagttt ttcatgtgcg aacggttcgc tttcatcaac aacggtcact
    24841 tcaccgatca gaatcttggt gttttctggt acagagaagc ctgccagttc agcaatttta
    24901 taggctggct gaccaacgat agccgcgttc agcgcaccgt ttttcaggat aacatcctga
    24961 acagctttca gctctttacc ctgcaacaga tagccgccgt gggttgcaaa acgttcacgt
    25021 acagcgtcat aaacagagtc aacaacaaca acagactgtt cagaagcaca gattacgccg
    25081 ttgtcgaagg ttttggacat cagtacagat gcaactgcac gtttgatatc agcagtttca
    25141 tcgataacaa ctggagtgtt gcccgcgcct acaccgatag ctggtttacc ggagctgtat
    25201 gcggctttaa ccatgcccgg accaccagtc gcgaggatca ggttgatgtc tgggtggtgc
    25261 atcagtgcgt tagacagttc aacagaaggt tgatcgatcc agccgatcag atctttcgga
    25321 gcaccggcag cgatagcagc ctgcagaacg atatcagccg ctttgttggt ggcatctttt
    25381 gcacgcgggt gcggggagaa gataatggcg ttacgggtct tcagactgat cagcgatttg
    25441 aagatagcag ttgaagtcgg gttagtggtc ggaacgatac cgcaaataat accgattggt
    25501 tcagcgatag tgatggtacc aaaagtgtcg tcttcagaca gaacaccaca ggttttttca
    25561 tctttatagg cgttgtagat atattcagaa gcaaagtggt ttttgatcac tttatcttcg
    25621 acgataccca tgccggattc ggcaacggcc attttcgcga gtgggattcg agcatctgca
    25681 gcagccagag cggcggcgcg gaagattttg tctacttgct cttgagtgaa actggcatat
    25741 tcacgctggg ctttttttac acgctctacg agtgcgttaa gttcagcgac attagtaaca
    25801 gccataatgc tctcctgata atgttaaact tttttagtaa atcatctgct cgaatacgag
    25861 agtatagtca gtgcggtgat gatttgctta acctatgaaa atcaaaagct tactcgcgct
    25921 cacactcact gtgatttact aaaagagttt aaacattaga gttattatct ctaatgcgtc
    25981 acttccaggt ggcgtaagca agattactca cttctgggta ctgattacgt gatccaaatc
    26041 aaatttttgc aaagctgaca cctttcagca tcgcttttcg ccattatagc taacagttaa
    26101 taaattgtag tatgatttgg tggctacatt agcatgtttt gcacaactag ataacaataa
    26161 cgaatgatag caattttaag tagttaggag gtgaaaaatg ctgtcaaaag gcgtattgtc
    26221 agcgcgtctt ttcaacctta tttatggcta acattatccg gcttttgctt cggagctaac
    26281 cgtgattcag accttttttg attttcccgt ttacttcaaa tttttcatcg ggttatttgc
    26341 gctggtcaac ccggtaggga ttattcccgt ctttatcagc atgaccagtt atcagacagc
    26401 ggcagcgcga aacaaaacta accttacagc caacctgtct gtggccatta tcttgtggat
    26461 ctcgcttttt ctcggcgaca cgattctgca actttttggt atatcaattg attcgttccg
    26521 tatcgccggg ggcatcctgg tggtgacgat agcgatgtcg atgatcagcg gcaagcttgg
    26581 cgaggataaa cagaacaagc aagaaaaatc agaaaccgcg gtacgtgaaa gcattggtgt
    26641 ggtgccattg gcgttgccgt tgatggcggg gccaggggcg atcagttcta ccatcgtctg
    26701 gggtacgcgt tatcacagca ttagctatct gtttggtttc tttgtggcta ttgcattgtt
    26761 cgctttatgt tgctggggat tgttccgcat ggcaccgtgg ctggtacggg ttttacgcca
    26821 gaccggcatc aacgtgatta cgcgtattat ggggctattg ctgatggcat tggggattga
    26881 atttatcgtt actggtatta aggggatttt ccccggcctg cttaattaat tcctttcaaa
    26941 tgaaacggag ctgccatgct ccgtttactt cgtcattatt tttactttgt tcccgcgcag
    27001 ttatcaaaag caaaaggaat aggtaaaata ttcttctcaa attacagtta gttataagga
    27061 tttccttaac tgcttctcct caccatcatg ttattttcgc cacatcataa tcctgggctt
    27121 gctgaagaat aattgaaatg atattagtaa ttccactgcc tttggtagag gaaagtgcta
    27181 aataataatc aattgttaaa ttattgtgca tttcactact ggaactgtaa tcagaaaaga
    27241 tagacatgct tagccaatct ctatttgatt gaattgaaag atgtttgtta aggcatggat
    27301 gcaagctata gattctgata cggtcaataa aagagaattg cttaacaatt ttgcaaaatg
    27361 tattggcgag taagaaccgc atttggtact ttccgggcaa ccgccagacg attctttatt
    27421 ggtaatgaga ataattaaca attaaagagc gtcgcgaaag aataatgtgt ctcgacaggg
    27481 gagacacagt acgaatcgac ataaggtgat cgtctgaatc accagaataa ataaagtcgg
    27541 tgatagtaat acgtaacgat aaagtaacct gacagcagaa agtctccgag cctgtgcagg
    27601 gtcccaatcc gggattacac atgctggtta ataccagtaa ttataatgag ggagtccaaa
    27661 aaacaatgac caacatcacc aagagaagtt tagtagcagc tggcgttctg gctgcgctaa
    27721 tggcagggaa tgtcgcgctg gcagctgatg tacccgcagg cgtcacactg gcggaaaaac
    27781 aaacactggt acgtaacaat ggttcagaag ttcagtcatt agatccgcac aaaattgaag
    27841 gtgttccgga gtctaatatc agccgagacc tgtttgaagg cttactggtc agcgatcttg
    27901 acggtcatcc agcacctggc gtcgctgaat cctgggataa taaagacgcg aaagtctgga
    27961 ccttccattt gcgtaaagat gcgaaatggt ctgatggcac gccagtcaca gcacaagact
    28021 ttgtgtatag ctggcaacgt tctgttgatc cgaacactgc ttctccgtat gccagttatc
    28081 tgcaatatgg gcatatcgcc ggtattgatg aaattcttga agggaaaaaa ccgattaccg
    28141 atctcggcgt gaaagctatt gatgatcaca cattagaagt caccttaagt gaacccgttc
    28201 cgtacttcta taaattactt gttcacccat caacttcacc ggtgccaaaa gccgctatcg
    28261 agaaattcgg cgaaaaatgg acccagcctg gtaatatcgt caccaacggt gcctatacct
    28321 taaaagattg ggtcgtaaac gaacgaatcg ttcttgaacg cagcccgacc tactggaaca
    28381 acgcgaaaac cgttattaac caggtaacct atttgcctat tgcttctgaa gttaccgatg
    28441 tcaaccgcta ccgtagtggt gaaatcgaca tgacttataa caacatgccg atcgaattgt
    28501 tccagaagct gaaaaaagag atcccggacg aagttcacgt tgatccatac ctgtgcactt
    28561 actattacga gattaacaac cagaaaccgc cattcaacga tgtgcgtgtg cgtaccgcac
    28621 tgaaactagg tatggaccgc gatatcattg ttaataaagt gaaagcgcag ggcgacatgc
    28681 ccgcctatgg ttacactcca ccgtatactg atggcgcaaa attgactcag ccggaatggt
    28741 ttggctggag ccaggaaaaa cgtaacgaag aagcgaaaaa actgctggct gaagcgggtt
    28801 ataccgcaga caaaccgttg accatcaacc tgttgtataa cacctccgat ctgcataaaa
    28861 agctggcgat tgctgcctct tcattgtgga agaaaaacat tggtgtaaac gtcaaactgg
    28921 ttaaccagga gtggaaaacg ttcctcgaca cccgtcacca gggtactttt gatgtggccc
    28981 gtgcaggctg gtgtgctgac tacaacgaac caacttcctt cctgaacacc atgctttcga
    29041 acagctcgat gaataccgcg cattataaga gcccggcctt tgacagcatt atggcggaaa
    29101 cgctgaaagt gactgacgag gcgcagcgca cagctctgta cactaaagca gaacaacagc
    29161 tggataagga ttcggccatt gttcctgttt attactacgt gaatgcgcgt ctggtgaaac
    29221 cgtgggttgg tggctatacc ggcaaagatc cgttggataa tacctatacc cggaatatgt
    29281 acattgtgaa gcactaatgg caatacgtgg ggcaggagtg tcctgctcca cggtgtctga
    29341 tttttatcgc attacagaag gcacaggcca gaaggtaggg caatgttaaa atttattcta
    29401 cgtcgctgtc tggaagcgat tccgacgcta tttattctta ttactatttc gttctttatg
    29461 atgcgcctcg cgccgggaag cccttttacc ggcgaacgta ctttaccgcc tgaagtgatg
    29521 gccaatatcg aagcgaaata tcatcttaat gatccgatca tgacacagta tttcagctac
    29581 ctgaaacaac tggcgcacgg tgatttcggt ccatcgttta aatataaaga ttattcggtc
    29641 aatgacctgg tggcatccag ttttcccgtt tctgccaaac tgggagccgc agcatttttc
    29701 cttgcggtaa tactgggtgt tagtgctggc gttattgccg cattaaaaca aaacaccaaa
    29761 tgggactata ccgtgatggg gctggcaatg accggggttg ttatccccag ttttgtggtt
    29821 gcgccattat tagtcatgat atttgcgatc attttgcatt ggctgccggg cggtggctgg
    29881 aatggtgggg cgcttaaatt catgatattg ccgatggtgg cgttgtcact cgcctatatc
    29941 gccagtattg cgcgtattac ccgtggctct atgattgaag tattacactc caattttatt
    30001 cgtactgccc gggcgaaagg gttacctatg cggcggatca ttttacgcca cgcattaaaa
    30061 cctgctctgt tacccgtgct ctcctatatg ggccccgcat ttgtcggcat tattaccggt
    30121 tctatggtta tcgaaaccat ttatggtttg ccggggattg ggcaactgtt cgttaatggc
    30181 gcattgaacc gtgactattc cttagtgtta agcctgacca tcctggttgg tgctttaacc
    30241 attttgttta atgccattgt cgatgtgcta tatgcggtta tcgacccgaa aatccgttac
    30301 tgatactgga gctcgcgatg atgttaagta agaaaaacag cgagacgctg gaaaatttca
    30361 gtgaaaagct ggaggtcgaa gggcgcagct tgtggcagga cgcacgtcga cgttttatgc
    30421 ataaccgtgc ggcggttgcc agtctgatag tgctggtgct gatcgcgtta tttgtaatcc
    30481 tggcaccgat gctttcgcag tttgcctatg acgatactga ctgggcgatg atgtccagcg
    30541 ccccggatat ggagtccggt cactactttg gtactgactc atccggtcgc gacctgcttg
    30601 tgcgcgttgc gattggcggg cgtatctcac tcatggtcgg tgttgctgcg gcactggtgg
    30661 cagtggtcgt cgggacactt tatggttcgc tttccggtta tctgggcggt aaagtggatt
    30721 cggtaatgat gcgtctgctg gaaatcctca actccttccc attcatgttc ttcgtcattt
    30781 tgctggtgac ctttttcggt caaaacatcc tgctgatttt cgtggcgata ggcatggttt
    30841 cctggctgga tatggctcgt attgtgcgtg ggcaaaccct gagtctgaag cgcaaagagt
    30901 ttattgaggc ggcacaagtt ggcggtgtat cgacatcggg cattgttatt cgccacattg
    30961 tgccgaatgt actcggtgtg gtggtggtct acgcatcact tctggtgccc agcatgatcc
    31021 tctttgaatc tttccttagc ttcctggggt tgggtacgca agagccgtta agcagctggg
    31081 gtgcattgct gagtgatggc gcgaactcga tggaagtctc tccatggtta ttgttgttcc
    31141 cagcgggatt cctcgtggtg acgctgtttt gtttcaactt tatcggcgat ggcttgcgtg
    31201 atgccctcga cccgaaagat cgttaaggag tgcagccatg agcgtaattg aaactgcaac
    31261 tgtgccgctc gcacaacaac aggctgacgc actgctgaac gtgaaagatt tgcgtgtcac
    31321 ctttagtacc ccggacggcg acgtcacggc ggtcaatgat ttgaattttt ccctacgtgc
    31381 cggagaaacg ctgggtattg taggtgagtc tggttcgggt aaatcgcaaa ctgcatttgc
    31441 gttgatgggc ctgttggctg ccaacggacg tattggcgga tcggcaacct tcaatgggcg
    31501 tgaaatcctc aatttgccag agcatgaact caataaactg cgcgctgaac aaatctcaat
    31561 gatttttcag gacccaatga cttcgttgaa tccctatatg cgcgtcggtg agcagttaat
    31621 ggaagtgctg atgctgcata agaacatgag caaagctgag gcgtttgaag agtcggtgcg
    31681 gatgctcgat gcggtaaaaa tgccggaagc gcgtaaacgc atgaaaatgt acccgcacga
    31741 attttctggc ggcatgcgtc agcgagtcat gattgcgatg gcattgctat gtcgacctaa
    31801 gctactgatt gcggatgaac ccactactgc gctggacgtc accgtacagg cgcagatcat
    31861 gacgctatta aatgaactga agcgggaatt taataccgcc atcattatga tcacccacga
    31921 tcttggcgtg gtggcgggga tctgcgacaa agtgctggta atgtacgccg ggcgcacgat
    31981 ggaatatggc aatgcgcgcg atgtctttta tcaacccgtt catccttatt ctatcggttt
    32041 gctcaacgcg gtgccgcgtc ttgatgcgga aggtgaaaca atgttgacca tcccgggtaa
    32101 tccgccaaac ttgctgcgat taccgaaagg ttgcccgttc cagccacgtt gtccacatgc
    32161 gatggaaatt tgtagtagcg caccgccgct ggaagagttt acgcctggcc gtctgcgtgc
    32221 ttgctttaaa ccggtggagg aactgttatg aatgctgtaa ctgaaggaag aaaagtcctc
    32281 cttgaaatcg ccgatcttaa agtgcacttt gaaatcaaag atggcaaaca gtggttctgg
    32341 caaccgccga aaacgctcaa agccgtcgat ggtgtcactc ttcgcctgta tgaaggggaa
    32401 acattaggtg tggtagggga atcgggatgc ggtaagtcca cctttgctcg cgccatcatc
    32461 ggtttggtca aggcgaccga cggtcatgtt gcctggttag gtaaagagtt gctgggcatg
    32521 aagcccgatg aatggcgtgc cgttcgcagt gatattcaga tgattttcca ggatccgttg
    32581 gcatcgctaa acccgcgtat gaccatcggc gagatcatcg ctgaaccact gcgtacttat
    32641 catccgaaaa tgtcacgcca ggaagttcgc gagcgcgtga aggcgatgat gctgaaagtc
    32701 gggttattgc ctaacctgat taaccgctat ccgcatgagt tctccggtgg gcagtgccag
    32761 cgtatcggga ttgctcgtgc tcttattctt gaaccgaagc tgattatctg cgatgagccg
    32821 gtttcggcgc tggacgtgtc aattcaggcg caggtggtca acctgctcca gcagctgcaa
    32881 cgtgagatgg gattgtcatt aatttttatc gctcatgacc tggccgtggt aaaacacatt
    32941 tccgatcgtg tgttggtgat gtatctcggc catgcggtag aactggggac ctatgatgag
    33001 gtctaccaca atccactaca tccttacacc agggcattga tgtcggcagt ccccatacct
    33061 gatccggatc tggagaagaa caaaaccatc cagttactgg aaggggaatt accgtcgccg
    33121 atcaacccgc cttccggttg tgttttccgt acccgttgcc cgattgccgg tccggagtgc
    33181 gccaaaacac gtcctgttct ggaggggagt ttcagacacg ccgtttcttg cctgaaagtc
    33241 gatccgcttt aaacacaata agggctgaca gttgtcagcc ctttttcacg ctaaaagcga
    33301 ttatttattc ccgccagatg atatggcaaa gtttgtgatc tttttcgcgg cataacagaa
    33361 tgcgggcgaa aacatcgttg atttcaccat cttcactgtc cgccagacca atcaccactt
    33421 cggcaaaaaa gtctgggttc aaatcgaaat caacatgttc ctgccagtct tccgccggat
    33481 cgaataactc cgcgccgccg cgctcttcaa actgaagatt gaacagcaga acatcagctg
    33541 gatcgagatt gtcggcagcc agttcgagaa aaatatcgta ggcctgctca agcgtttcat
    33601 cttcagtcag gcgattgttt agatccatat ccatagtcac tacctgttta acctctgttg
    33661 gcgacgtttt acagcaacgg actgaagaag taaaacagtc gctcggcgac acgttgccat
    33721 aatggacgtt ttagccataa acgggcatcg agcagacgtg aacgcgaaat ataatcgtcc
    33781 tgaacggcgg caaggtctgc accaaaacct ttatcgtcga ttgccagggt aatctcgaaa
    33841 ttcagccaca gactacgcat atcaaggtta acggtgccaa ccagacttag ttcgccatcg
    33901 accagcacgc tcttggtatg cagtaacccg ccttcaaact gataaatttt aaccccagca
    33961 gcaagaagtt ccgtaaagaa tgcgcgactg gcccagccga ccagcatcga gtcatttttt
    34021 cgcggaagga taatactgac atccaccccg cgctgcgccg ccgtgcaaat cgcatgaagt
    34081 aaatcatcgc ttggcacaaa gtagggcgtg gtcatgatca aatattcacg cgccgaataa
    34141 gccgcagtca ataatgcctg gtgaatgaga tcttccggaa agccggggcc agaagcaatt
    34201 gtgtgaatgg tgtgaccgct ggcctgttca aacggcataa tattgacatc tggtggtggc
    34261 ggcagaatac gttttccggt ttcaatctcc cagtcgcagg aataaataat ccccatcgcg
    34321 gtggcgatgg ggccttccat acgcgccatc agatcaatcc attgccctac gcccgcatct
    34381 tgtttgaagt agcgaggatc gaccatattc atgctgccgg tgtacgcgat gtaattatcg
    34441 atcatgatca tcttgcgatg ttggcgcagg tccatacggc gtaaaaacac acgcatcaga
    34501 ttgaccttta aggcttcgac cacttcaata ccggcattac gcattagctc gggccacggg
    34561 ctgcggaaaa aagccacact cccggcggag tcgagcatca atcggcaatg aataccacgc
    34621 cgtgcagccg ccattaatga ttcagccacc tggtccgcca tgccgccggg ctgccagata
    34681 taaaacacca tctcaatatt atggcgcgcg agctggatgt cgcggattaa cgcctgcatc
    34741 acatcatctg actcggtcat cagttgtagc tgattccctt tgaccccagc gatcccctga
    34801 cgacgctcgc aaagcttgaa taatggcgca gcgacactgc tattttcttc ggcgaagata
    34861 tgcttacagg ctttaaggtc gttaagccat tttgcggtgg aaggccacat cgctctggcg
    34921 cgctcagcgc ggcgtttgcc taaatggagc tcgccaacgg caagataggc aataattccg
    34981 actaacggca gaatgtaaat aatcaacagc caggccatcg cggagggaac tgcgcgtcgt
    35041 ttcattagaa tgcgtaaagt tacgcctgca atgagcaacc agtatcccag aatggccaac
    35101 caactcacca acgtataaac ggttgtcata gattagaaat ccttttgaaa gcgcattgtt
    35161 atgagtttac gcatatggat tcatctggca aataaaaacg cgggaaaagc actggtcagc
    35221 gtcgggggtg ggggtataat gaccattctg ttattgcata gagtagttaa catgaagcgg
    35281 agtagaacgg aagtggggcg ctggcgcatg cagcgtcagg ctagccgacg taaatcgcgt
    35341 tggcttgagg ggcaatcgcg ccgaaatatg cgtatccaca gcatcaggaa gtgcattcta
    35401 aacaaacagc gtaactcgtt attgtttgcg atctacaata tctaaatata ctgggcacca
    35461 ttgcggtgcc caataagttt actctttcgt ctttaaactc tatttttgca ctgattcttt
    35521 actgtcagct ttcgtttctt gttcatcgtt atcgctgaaa atgccatgac cggagttcaa
    35581 caacattgaa acgagcatat cattattaat ctcttcacca tttaaaactc gcgccagaat
    35641 ttcgctgcca aacagttgcg gtgagagaat gatatccgga tgtaccatct taatcttatt
    35701 caggtttttg ctatcactga cggcgagaac tgttttgaca tcactgctca tatcttttgc
    35761 cgagagtaca acaaacgcgt tatctgcatc gttatcactc agcgccagaa tggcccggca
    35821 acgatcgatt cccgctttct ttaatactga actgtcatta ctgtcaccgg ggataacatc
    35881 agcgttatcg cctaagcgtt gctcaagttg cttgatatca tcttcaggca agttgctgat
    35941 aaccgttacg ttttgtccgc gttgattcag ttgcaggatc gtattgatgg cgagaatcga
    36001 atgtccgcaa acaataaaat gatctttacg atgcattgta tgattgtttc cttttacaag
    36061 tttgttgaat cccccgcgga taagcggacc aaaaattgag gtcatagatg tggcaaaaac
    36121 ggtaatgccg gaaataatga ccgaaatagt gaacaatcgt gctgattcag aaacagggac
    36181 aatatcgcca tagccgacgg ttgacatggt ttctatcgaa aaatagaacg cggtcatcaa
    36241 actttctatt cgcggattaa aaccttcgct taaataaagc gcaccgtagg ttgagtaaaa
    36301 cagtaatgtc gtgaaactaa taaatgcaaa aattgtcccg gctgcggcgc tactgtggga
    36361 gaagtctttg cgcagtatca gcaaaaacac cagcgtaaaa atgcaaaatc caatactaaa
    36421 tttcagccag ggataaaaat gcagggtgta aattagcgct atcaacaaca aaatgatact
    36481 gattgcccat gcgagctttg cccggaacag taagccaatg gagttcagga caagaaagac
    36541 gccgagcata aatagtgggg cgtttgccag caaggagaga tccaactctg aaaatgcttt
    36601 gatatggaaa atatcaagca aattgaccga cataccatag attgttttaa aaataagcaa
    36661 tccatttaaa aagacggcca gcgcgagaat gtcgtgccgt agggtcaccc ataaatttgt
    36721 tgctgtttgt ttgaatgtag cccagtgact cactgatacc tctgtaacca caattttgtt
    36781 taattatctt agcgtgcaca ttttctgaac atacatgcag cgcgtgaatg tgttaaacaa
    36841 tagtagatga ctttatcggc agtaatcaaa ggttttgagc gagcaactct gcgtcaggaa
    36901 agggcatttt cctgaatatg ttcacatcat ataaagaaat acagataaag ttattatctg
    36961 cttgtggtag tgaatgcact gaccggctat aaggaaaggc caaacaagaa cacggttgca
    37021 aaaaccgtgc ccttaaatat tgaatctcta ttcagaacac tttcttaaat ggtttcactg
    37081 aaacgtgttc atagacacct gccgctacgt acgggtcagc atcggcccag gcctgagctg
    37141 cttccagcga ttcaaattca gcaataacgg ttgagccagt aaatcccgca gcccctggat
    37201 cgttactgtc taccgctggc attggaccag ctgtcaacaa acgaccttca tcatgcagta
    37261 actgtaaacg tgctaaatgt gccggacgaa cggaaaggcg tttttcgagg ctatcagctt
    37321 tgtcttgagc gtaaataaca tacaacacgg gcacaactcc ttgttcggga aagttgtaaa
    37381 gtacgttatt tgaaagggcg aagatctgca acggaaagat gatgtctttg ttaagaccat
    37441 gcataaagta aggtcaatta cgccaaaaat gacattttca ctgatcctga tcgtcttgcc
    37501 ttattgaata tgattgctat ttgcatttaa aatcgagacc tggtttttct actgaaatga
    37561 ttatgacttc aatgaccctt gatttacctc gccgcttccc ctggccgacg ttactttcgg
    37621 tctgcattca tggtgctgtt gtggcgggtc tgctctatac ctcggtacat caggttattg
    37681 aactacctgc gcctgcgcag ccaatttctg tcacgatggt tgcgcctgct gatctcgaac
    37741 cgccacaagc cgttcagccg ccaccggagc cggtggtaga gccagaaccg gaacctgagc
    37801 cgatccccga accgccaaaa gaagcaccgg tggtcattga aaagccgaag ccgaaaccta
    37861 agccaaaacc gaagccggtg aaaaaggtac aggagcagcc aaaacgcgat atcaaacccg
    37921 tagagtcgcg tccggcatca ccgtttgaaa atacggcacc cgcgcgcctg acatcaagta
    37981 cggcaacggc tgcaaccagc aagccggtta ccagtgtggc ttcaggacca cgcgcattaa
    38041 gccgtaatca gccgcagtat ccggcacgag cacaggcatt gcgcattgaa gggcaggtta
    38101 aagttaaatt tgacgtcacg ccggatggtc gcgtggataa cgtacaaatc ctctcagcca
    38161 agcctgcgaa catgtttgag cgtgaggtga aaaatgcgat gcgcagatgg cgttatgagc
    38221 cgggtaagcc aggcagtggg attgtggtga atatcctgtt taaaattaac ggcaccaccg
    38281 aaattcagta agcagaaagt caaaagcctc cgaccggagg cttttgacta ttactcaaca
    38341 ggtaaggcgc gaggttttcc ttcaggatca accgcgacat acttaaataa tgcttctgtc
    38401 gctttatagc gttgcccgat tggttcagac gctacttttt tcacccacac ttcaatatta
    38461 atgctgaccg atgtcgtccc tttctggaca cagcgtgcat agcagcacac cacatcgccg
    38521 accgcaaccg gccgtaagaa agtcattcct tcaacccgca cagtcactac gcgaccgtgg
    38581 gcaatttctt tcgccagaat agcgccgcca atatccattt gtgacattaa ccaaccacca
    38641 aagatgtcac cattggcatt ggtatcggcg ggcatggcta aagtacgtaa aacaagatcg
    38701 ccctgaggga cgttatgtgt tgtagacatg gtaaaaccga cttaaaagga aaatcacagg
    38761 cgcgatgcta ctatgatttt cctgaggaga acagaggaga tacagcgcca gccccgaagg
    38821 actggcagtc tggttaggat ttatcttcct gcggcatgtg gcggtagata tagataccgc
    38881 ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa gactttaaag ttgacccaaa
    38941 tattttgcgg cagccagaat gcgatgtaga tgtttgccag accgcaaagg ataaagaaaa
    39001 cagcccaagc cagatttagc ttcgaccata ccggttgcgg cagcgtgagt tctttaccca
    39061 gcatccgctg aattagcggc tttttcatca cccattggct gactaacagg gctcccgcaa
    39121 acagagcata aatgactgta accttccatt taataaactc atcattgtgg aagaacagcg
    39181 tcaagccacc gaagacgacc accagaacaa aagtgatcag ggccatcttc tcaaccttac
    39241 gaaagcgaac ccagctatag ataagcacaa tcgccgtggc gacgatcagc gccgcagtag
    39301 ccgcgtagat gtcataaatc ttgtaaaacg cgaaaaagac aaccagcggt aaaaaatcaa
    39361 gaaactgctt cattttacga ttccgtaatc aaatgcttaa ggttattggc gaatcaacat
    39421 atacaggcgg aacagataga tgagcaatat ggcagaaatc aggttgctca aggtgttcgc
    39481 cagtacggca ccaatttccg gggttaatgc ggcaaaagaa gaggcaaaaa gcagcaacag
    39541 tgtttttgcc agcaaccagc tcagtactgc gggtgccacc agacgcatat tcgcccaagt
    39601 cagccgcata ctgctacgca tcgaggcaaa aatgcccatt ttgtcctgta ccagcatcac
    39661 cggtgccagg gccagtaaaa tggccatgat aattcccgga acgaccacca gcatgatgcc
    39721 aatctgtact aaaagggtag tcagaaaaat cagaataaat aacttgggca atatcggcgc
    39781 actggcacca atagctcgca gcgcactgac tctctgaccc gcagacacca gctggataat
    39841 taatattacg cctccggcga gaatggcgtt accgattaat cctgaaaaag tggacgccgc
    39901 tgaagcctgc agcaaaattt gttgctgttc cggtgacata ttctgaacca ggtcgaacaa
    39961 cccactactg ccgctaacgg gcacgccgtc attgagctgc gcaagctgtg catcactggg
    40021 tgagaaaaca tgccctaaca ccactgtgat aaacgcacat agcaacgata ccaacagaat
    40081 ggtcataaat tgattacgga agaaatttcc ggtgtcacgg tatacggact gcgccgtgat
    40141 agacatgcac tctccttgag ttttatgcag gtgttaatta gcgggcaatt gtaccctggt
    40201 taagcctcgg gtggcagcat caggtcttgt atggaaaagc atatctttgt aaagcggagg
    40261 taattgctgg ccttcaggcg tgggcaaacc gcggcaagtg gcgatcgagt taatatttgc
    40321 gtagagaaaa tatttaaaaa ttgatttaaa tcacattaac caggattctc aatgcaactt
    40381 ctaaattaat ccagatcaat aaatggtgaa ttatcatatg taatgtgatc tatgtaggat
    40441 catttgttac tccaatgtag gtatattcgt cacgttttta taaccataac gacggagcgg
    40501 atatgaaaaa gttaacagtg gcggctttgg cagtaacaac tcttctttct ggcagtgcct
    40561 ttgcgcatga agcaggcgaa ttttttatgc gtgcaggttc tgcaaccgta cgtccaacag
    40621 aaggtgctgg tggtacgtta ggaagtctgg gtggattcag cgtgaccaat aacactcaac
    40681 tgggcttgac gtttacttat atggcgaccg acaacattgg tgtggaatta ctggcagcga
    40741 cgccgttccg ccataaaatc ggcacccggg cgaccggcga tattgcaacc gttcaccatc
    40801 tgccgccaac actgatggcg cagtggtatt ttggtgatgc cagcagcaaa ttccgtcctt
    40861 acgttggggc cggtattaac tacaccacct tctttgataa tggatttaac gatcatggca
    40921 aagaggcggg gctttccgat ctcagtctga aagattcctg gggagctgcc gggcaggtgg
    40981 gggttgatta tctgattaac cgtgactggt tggttaacat gtcagtgtgg tacatggata
    41041 tcgataccac cgccaattat aagctaggcg gtgcacagca acacgatagc gtacgcctcg
    41101 atccgtgggt gtttatgttc tcagcaggat atcgttttta attccgcaca aaaacgaccc
    41161 cgtaatatac ggggtcaata aggacatggt ataaagggta ttatttcttc gcttctacgc
    41221 catcagtttc agaacgaatt aaaaatttct cagttgtttg cggaatatgc tgaatcagcc
    41281 agtcggccat ttgcttttcc tcatttaaaa tcgcttcgat gatcggaatt gagactgtat
    41341 caccggcatt ttttgctgct gctaatagtg aggtgtaaca ggcgatttca aattgctcga
    41401 agacatatcc gctaatagag cctttaacta tttcatcaga agggaatata ccaccgattg
    41461 actgcccaag cgcagccatt ttactcatgg aatctttaat gactgaacgt gaaatgtcat
    41521 tacgatcaag aatagtttcc agttgaacaa tctggttttt ggtttcacta agatgttgtt
    41581 caatacgagc gcgtagttca ggataattat ctatacgact ggccatggat tcaagcatag
    41641 attcggcttg cttttccatt gcgtgggcgt cacgtaacca gtcatgataa tgttcaatac
    41701 gattcatgaa aatgtcctct ttataaaatg actaaaagtt aaaattcata tttcaggctt
    41761 tattttcggc tttcttattt acgttattaa gggccagatc agtcagtttg atgtcggtgg
    41821 ccttttcttc ttccagggtt tctttcagaa gcttcgctgc tttacggtaa cctaattgtt
    41881 cagccagcgt cgctaatgtc ccgtaactgg caatctcata atgctcgact ttctgtgctg
    41941 cggcaatcag tgcggcatca cgcacttcgt ttttctcggt actttcgatg acctcattag
    42001 cttcttcaat aagaccttcc attgccacac atttcatgcg cttaattttc aggttcgatt
    42061 cggactccac aacttgatca atacgttcaa tctgtccatg agtttcctcg aggtgcgcat
    42121 gaaaagcctg acttaatttt tcatttgatg ttgctcttgc gagttttgcc agtgcccggg
    42181 ttaattgttt ttctgcgctg taggtatctg aaagcaggtg aataaataca tcttcaatgg
    42241 tcttcatatt catatttttc tccagtgaaa tcactgcgag ccatacggcc cgcagatgca
    42301 attatcttta tcagctgata ttaataatta acggtgaatc aggatttacc tgatttatta
    42361 ccaccgcttt gttgaccgcc ttttttaccc gcttcagatg cgcgttgcgg atcattttta
    42421 aaattaccgc cgctatgctg accgccttta cggcctgcgt cggatgcctt ctcacggtct
    42481 tcggcgaaat ttcctgaacc accacgatgt tcggccatgt tatttctccc gttgcgttgc
    42541 attgtttcat taatatgagt gttgtgtgtc gacactcatt aaaattagtc gctaatgaga
    42601 attagtcaaa ttaagcgcaa cgagaagata gagggaaaat atattttgag gaacattctg
    42661 gatatattaa caattacctg aggaataagt gacttagaga aaattattga ttttactggt
    42721 gttatgttgc gggattaatt tgtttgatta tattactgtt gggcggaaaa atgacgtaag
    42781 ttgacgttcg accggggtaa gcgaaacggt aaaaagataa atattaaatg aatttaggat
    42841 ttttccggct tcattaaaga aagttaaaat gccgccagcg gaactggcgg ctgtgggatt
    42901 aactgcgcgt cgccgctttc atcggttgta caaaaacttt cagtgccgcc agcattttct
    42961 ctggctcatt aatatgttgc tcgatgattt taacaatggc cgaaccagaa atcgcgcccg
    43021 cagctcctgc atcaatcgct gcttttacct gatccggggc ggaaatacca aacccctgca
    43081 atggaggtgc agcgttgtac tctttcagct tcgcaaccag atgattgagg ggtaacgcgg
    43141 cgcggttttc tgcgccggtc acgcccgctc gtgacagcaa ataggtgtaa ccacgaccgt
    43201 aagaggctat ctggcgcagc aggtcgtcat cggcatttgg cgggcagatg aagataggtg
    43261 cgacattatg gcgcaacgcg gcctggcgga agggcgcgga ctcttcaatt ggcacatcgg
    43321 caaccagcac cgaatcgacg ccgactttct cgcactgggc ataaaactca tcaatgcctt
    43381 tgttaaacac cagattggca tacatcaaca ggccaatggg aatggtcggg tgtttctggc
    43441 gaatcagtgc cagcatttca aaacattgtg ccggagtcac acctgccgca aaggcgcgca
    43501 gagtggcgtt ttgaatcgtc gggccatccg ccagtgggtc ggagaagggg atacctaact
    43561 ccagcgcgtc agcaccggct tcaattagcg tatcgataat tttcaatgac tgctcaatgc
    43621 ccggatcacc gagcgtgacg aaaggaacga atgcgccttc tttgcgctcc ttcaactggg
    43681 caaacagaga ttcgtagcgt tccatcagat ttcccctcgt gctttcaaaa tatcgtgaac
    43741 ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc accagtagct gctctttatc
    43801 cgggttttcg cgcatcattt tcaacgcatg ggccagggcg tgggaggatt ccagcgccgg
    43861 gatgatccct tcgtgcaggc acagcgtttt gaaggcttca agggcttcat catcggtaat
    43921 agacacgtaa tcagcgcgtc cagtgctgtt aagatacgcg tgttgtgggc cgacagacgg
    43981 gaaatccagt ccggcggaga tggagtaaga ttcttcaatc tgcccgtctt cggtttgcat
    44041 catcggcgct ttcataccga aatagatgcc cacgcgacca tgttttaacg gtgcgccgtg
    44101 ctcgccagtt tcgataccgt gaccacctgg ctccacacca atcaggccga cgttggtttc
    44161 attgatgaaa tcagcaaaca tgccgatggc attcgaaccg ccgccaacac aggcgataac
    44221 ggcatccggc aggcgacctt ctctttccag aatctgcgct ttggtttctt cgccaatcat
    44281 ccgctgaaac tcacgcacaa tggtcggata aggatgcggg ccagctgcgg tgcccagcat
    44341 atagtgcgcg gtttcgtaac taccggacca gtcgcgcagc gcctcgttac aggcatcttt
    44401 cagcgtcgcg gaaccgctat gcaccgggat cacttccgca cccattaagc gcatacgaaa
    44461 aacgttaggc gactggcgtt ctacgtcttt ggcacccata taaatacggc atttcaggcc
    44521 gagcagggcg ctggcaaggg ccgacgccac gccatgctga ccggcaccgg tttcggcgat
    44581 gatttcggtt ttacccatcc gcttcgccag taaagcctga ccgagcacct ggttagtttt
    44641 atgcgcgccg ccgtgcagca aatcttcacg cttgagatac agagtggtgt tcgtcccggc
    44701 tgtaatgttc tggcatttgg tcagcgcggt tggacgcccg gcatagtttt tcagcaggtc
    44761 gttgaactga gcctgaaatt caggatcttt ttgcgcactg acaaaagctt cttccagctg
    44821 gcgcagagca ggcatcagga tttgtggcac gtacatgccg ccaaactcac caaaataggg
    44881 gttaagtaat gttgtcattg ttcctttcct taatatgcgc gcagcgtctg gaaaaccgag
    44941 gccaaaagac gtgcgtcttt gatgcccggt tgcgactcta cagcagaatt aaaatcaagt
    45001 ccggcgcagc cggtttgtgc cgcttccacg cagttatctg cgcctaagcc ccccgccagc
    45061 agaacgttgc caagcgattg accatttaat agtgaccagt cgaaacgttg cccgcttcca
    45121 ccctggccgt tgtctaaaac atatttatca acgtgctgaa gctcgcgggc gggcagggtt
    45181 tcaccgacgc ttaatgcttt ccagatggca acgtgtgctg gcagagcttc acgcagcgta
    45241 tcgatataca gctgatcttc attaccatgc agttgcactg ccgccagcga taacacctta
    45301 gctttgtcca ccacatcggc aatatcgtga ttgcggaaca cgccaacata ctgcaacggt
    45361 gccgcagcca tcacttcctg cgcctgttca acgttgacgc aacgcggtga tgtcgcaaca
    45421 aaaatcaacc caccgtaaat cgcgcccgcg tcataagctg ctttagcatc ttgcccacgc
    45481 gtcaggccac atactttatt ctcacccagc aacacccggc gcacggcggc gtgcaaatcg
    45541 tcatgggcca tcaacgccga accaatcaga aaaccgttag cgaagtggct taactcgcgc
    45601 acctgagcgt aagtattgat gccggattcg ctgattaccg ttacgttgtg ccccagtttc
    45661 ggcgcaagct cgcgggtacg gttgagatca atcgacaaat cccgcagatc gcggttgttg
    45721 atgccaacga cctttgcccc caatgcaatg gcacgctcca gttcctcttc attactgact
    45781 tcggtcagca cacccatctc cagactgtgg gcgacggcgg caagctggcg atattgttcg
    45841 tcatccagta ctgaaagcat taataagcag gcatcagcct ggtaatagcg cgccagatag
    45901 atctggtaag gatcgataat gaagtcttta cataaaatcg gctgcggggc gatttggctg
    45961 acgatgggga ggaaatcaaa gctcccctga aaatatttct catcagtcag caccgaaatt
    46021 gccgaagcgt aatgtttata aatggcggca atgcgtgctg gatcgaaatc atcacggatc
    46081 acgccttttg acggcgacgc tttcttgcac tccagaataa acgccgtgcg cgcaccctgt
    46141 agcgcatcat aaaaatgtcg cgtgctcggc tgaacctcat tctgaaaact ggcccgcggt
    46201 tgctgctgtt tgcgggtttc tacccaaatc gccttgtctg cgacgatttt cgctaaaacg
    46261 gtttgcatca tttaccctcg tgccgccagc gcggtaactc tgtcgtaagc ggaaccactg
    46321 cgcagtacct caagaacggt ttgcgcattg gcttgcagat cttcatggcc atgcaggcgc
    46381 attaacatgg cgacgttcgc agcgacggct gcttcatggg cggcgtcgcc tttaccttgt
    46441 aacaaacgtg ttaaaatgtc acggttttct tccggtgttc cgcctgccag ttgctcctgg
    46501 tggtagggtg tcaggccaaa atcttcagcg gtcaattgat agctcttaat ttcgccgtca
    46561 tgcagctcgg caacgattgt cggcgcgtgt aatgaaactt catccatccc gccgctgtgc
    46621 accaccgccg cgcgttgata ccccagcacg cgcaaggttt cggcaatcgg cagcaccagt
    46681 tccggactat aaacaccaat taacgccagc ggcggatgcg ccgggttaat caatggcccc
    46741 agcacattga acagggtgcg ggtttttagt tgctggcgaa ccggcattgc atggcgaaat
    46801 ccggtgtgat atttcggtgc aaagaggaaa catacgccta actcatccag cgcctggcgc
    46861 gatttatcgg cgttcatatc aagattaata ccgaacgccg ccagcagatc ggacgaacca
    46921 gatttactgg agacgctacg gttgccgtgt ttcgccactt tcagcccaca ggccgcggcg
    46981 acaaacgcac tggcggtaga aatattgata ctgttgctgc cgtcaccgcc agtaccgacg
    47041 atatcagcaa acagataatc cgggcgcggg aacggcgctg cgttttccag tagcgcggtt
    47101 gctgccccgg cgatctcgtt cgggtgctca ccgcgaattt tcatgctcac cagcgccgcc
    47161 gccagttgtt ccggcttcag ctcgccacgc accaccgctg aaaacagctg gtggctttct
    47221 tgttggctaa gcgtctgcgc ctgatacagt ttttccagaa tcggttgcag cgtgttggct
    47281 ggctctagtt tctgctgcgc ccaggccagc gtttgttcca gcaggcgagc gccctgggtg
    47341 gtgagaatgg attccggatg gaactggaat ccacaaacgc gatccgcatc gtgacgtact
    47401 gccatcacca tgccattaaa atgggcgttg atggttaaac cggccggaat gttactgcca
    47461 accagcgagt gataacgcgc caccggcagc gggtttgtta atccggcaaa catcgcctga
    47521 ccgtcatgtt caatgctcga cgctttaccg tgaagaattt cgcccgcctg accgacatag
    47581 cccccgtaag cttcgacaat tgcctgatgt ccgaggcaaa tgccaataat gggcagcttg
    47641 ccacgcaagc gggtgaggag ttccggcata caaccggctt cgctcggcac accggggcca
    47701 ggagaaagca tcagtaccgg attgctcatg gtcgccaggc gttcaattaa ggtctgcgcc
    47761 ggaatatggt tgcggtaaat caccacgtta tgcccattgc tgcgcaactg atctgccagg
    47821 ttgtacgtaa aagagtcgat attatcgagc agcagtatgt cagccatcag aaagtctcct
    47881 gtgcatgatg cgcggtggca atagcgcgca gtacagagcg ggctttatta cgggtttcgt
    47941 cggcttccga ctgcggaata gaatcaagga ctacgccagc accggcttgc acggtggcga
    48001 taccgttttc caccagcgcc gagcggatca caatgcaggt gtcgagatcg ccgtgcgcgg
    48061 tgaaataacc taccgcgccg ccgtagctgc cgcggcgacg accttccgcc tcggcaatta
    48121 actgcatggc gcgcactttc ggcgcaccgc ttaacgtccc catattcata caggcgcggt
    48181 aagcgtgtag ggcgtcgaga tcgtggcgca gctcaccgac cacgcgggag accaggtgca
    48241 tcacgtaaga gtaacggtca actttggtga ggtcggcgac gtagcggctg ccgggggtgc
    48301 aaatgcgtgc cagatcatta cgggcaagat ccaccagcat cagatgttca gaaagctctt
    48361 tatgatcggt acgcatttcc agttcgatgc ggctgtcgag gtctctgtcc agcgagccat
    48421 cggcacgacg accgcgtgga cgtgttccgg caatcgggta gatctcaatc tggcggctgg
    48481 tggcgtcata cttgagcgaa ctttccggcg acgcgccaaa cagggtgaaa tcattatcct
    48541 gcataaaaaa catgtacggg ctgggattac tctttttcag cacgtaatag gccgccagcg
    48601 gtgacgggca gggcagagag aaacggcgag atggcaccac ctggaaaatt tctccagcgc
    48661 gaatcgcttt ttgcaacaaa cgcactacgc caccgaactc ttcatcgctc tgattacatt
    48721 cacaacgcat atgcggcacg gaaaccaccg gcagcggcgg cgcggcttcg gtcagttgct
    48781 gacgtagttc gttcaggcga gcagtgagac gttgtttttc ttcttcattc ggagcaaaca
    48841 ggctggcctg aatacgggtg ctttttttct gatggtcaat caccatcaga gtttcagcga
    48901 gataaaaaca gaaatcaggg cagttatttt ccgctgacag ttgcggtaaa tcttcaaatc
    48961 ccgccacaag gtcataagag aacaggccgc cgaagaacat tgcttctcgt tcttccttcg
    49021 gtacattcaa cagattctgc aataaacgga aagcgtcaaa aaccgaaagg gagcataagc
    49081 gagcgtcttc atccagcagt ggactgacag gggggaagcg cagcacacgg cagtttggta
    49141 attgttcatt ttccacaccc gcaggcaggg cgttatccag tagtgtcagc agggcttcgc
    49201 cgttgccgga aagtgcctgg attgtgacag tgtcacctaa agctgtaatg cgcagcgcac
    49261 tgtctaccag tagcaggctt tttaaatcat ctttgctgtc gatatctgcg gattccagca
    49321 gcagcgttgc cggacgatcc ccacacaact ggtgaaaaag cgcggtggga ttgtcgcgat
    49381 aagcgccttc gcaggttagc agttcgagag tcggtttttg tgtttgcatt gttattctct
    49441 aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg gtatctgatt gctttacgca
    49501 tggtgaatac actgcccgtt tcaggaagtg cgccaccaac ctttcagtac gaaaattgct
    49561 ttcattgtcg ataccctttt tacgtgaact tgcgtactag ttaactagtt cgatgattaa
    49621 ttgtcaacag ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca
    49681 ttatccagaa cgggagtgcg ccttgagcga cacgaattac gcagtgattt acgacctgca
    49741 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
    49801 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc
    49861 agcaagagag gaaatttcac gttcgggact ggcgctgaat cttattcccg gcgtggaaat
    49921 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
    49981 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagct
    50041 gattgccgaa agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact
    50101 ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgttg agtgcggcaa
    50161 agccagttca atggcggatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
    50221 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
    50281 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
    50341 tgcgcatttt gccgaacacc acggtgacgc gatggaagtc gcgcagtgcc agcaatcgcc
    50401 caatgaacgt acccagctgg cggcccttgc gcgtcagcat catttatggg catcacaagg
    50461 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
    50521 aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
    50581 atgagccagt ttttttatat tcatcctgat aacccacagc aacgtctgat caaccaggcg
    50641 gtggagatcg tgcgtaaagg cggggtgatt gtttatccaa ctgattccgg ctatgcgctc
    50701 ggctgtaaaa ttgaagataa aaacgcgatg gagcgtattt gtcgtattcg ccagctgccg
    50761 gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattt
    50821 gttgataacg ttgcgtttcg tttaatgaaa aacaacacgc cgggcaacta taccttcatc
    50881 ttgaagggga cgaaggaagt gccacgccgt ctgttgcagg aaaaacgcaa aaccatcggt
    50941 atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg
    51001 ctttccactt cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt
    51061 aaagaccgtc tggaaaaaca ggtggatttg attatccacg gcggctatct cggacagaaa
    51121 ccgacaacgg ttattgatct taccgatgat acgccggtcg tggtgcgtga aggcgtaggt
    51181 gatgtgaagc ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc
    51241 tatacggcgc cattgattct cctgttcgac gcctgtgaag gcgacaccaa aaggaagctc
    51301 tatgagcgaa aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat
    51361 cgaatctatt attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga
    51421 tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg
    51481 tgagtccgct gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg
    51541 tacccgtaac gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg
    51601 cgcacgctgg attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac
    51661 caccgatggt gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata
    51721 cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt
    51781 gcaactggaa gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat
    51841 caaccagtgg tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg
    51901 ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact
    51961 gccaaaaggt ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct
    52021 gcgcgaactg gtggagcttc cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg
    52081 tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg
    52141 cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg
    52201 gcatttttac agattaataa tcaatcccta tctgcgcttt tacaccggca tcaaacgcat
    52261 gtttgacggg gcgtaattca cttaccgtat ctgccagttc aagaatatcc cgatgacaac
    52321 cacgacccgt gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt
    52381 cttccagtgg caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat
    52441 ccagtgagga atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg
    52501 tatcagactc gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa
    52561 cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca
    52621 cgacgcctac tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg
    52681 ttttgccttt tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg
    52741 ccacacgagc atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac
    52801 tcattgggaa attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg
    52861 gcggctgtca tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag
    52921 tttctgtgga tcttcggtcg ggaaggcgct ggcacgcatt gcggtgcgcg taccgcctgg
    52981 gttaatgcag ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc
    53041 ttcggtggca aatttcgacg ctgcatatgc gccccagttg gctcgtccct gacgtccaac
    53101 gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc
    53161 ctgggtgagc ataaaggtgg cattaacgtt gacctgtatg acgtcctgcc agacctgtgg
    53221 attttgttcg ctcattgggc aaacatcgcc gagcaatccg gcattatgca aaacaccatc
    53281 cagacgcgga taattaacgg taatgcgctg tgccagttgt tggcaatctt cggacgtgca
    53341 ggtcagcaaa tcgagaataa accactgtgg ctgacgtcca gtttcttcgt ttatgtggct
    53401 ggctacctga cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg
    53461 tgcatacgtc atcgcggctt cacggccaat cccatcgctg gctcccgtca ccaggataat
    53521 gcgatcattg agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg
    53581 tctgtcgtgc tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca
    53641 tttcaatcag cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca
    53701 ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt
    53761 taaccaaggt ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggcgaaaatc
    53821 gttaccgttg tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt
    53881 aataaacgcc agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg
    53941 aaagaagaac tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg
    54001 cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag
    54061 gtggcaactg acagtaaacc tcgcgtctgg gtgctggatt ttaaaggcag catggacgcc
    54121 catgaagtga actcgctacg tgaagagata acggcggtac tcgcagcatt caaaccgcag
    54181 gatcaggttg tgctccgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg
    54241 gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa
    54301 gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg
    54361 tttgctattg tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg
    54421 aaaagcaaag atattgatat cgaactgcac actgccgggc agtataagcg tactctgacg
    54481 ttgctgggtg aaaataccga agaagggcgg gagaaattcc gcgaagagtt gaacgaaacg
    54541 catcagttgt ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg
    54601 gcaacgggtg aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc
    54661 aacaccagtg atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc
    54721 tatatgcagc gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat
    54781 cgattgttgc tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg
    54841 aggctaagac ctcgcgtttt gctttaatca accagatgat atttttctga aagcacatgg
    54901 gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca
    54961 tctaaaaagt agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct
    55021 tcaatccctg cgagcgtatc ctcatgctca cgaatgattt tgttggcctc tttcaacagc
    55081 gtttcgcggt cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt
    55141 agtctacgcc aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac
    55201 tggtaacttt ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg
    55261 tgacgctttc gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc
    55321 tatagcgcct gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc
    55381 acgacaaggg gttgatatcc gcagagaacg agtccatatc ggtaactcgt tgccagtgga
    55441 aggtttatca acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt
    55501 aaagctcttg tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt
    55561 agtgactacg tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca
    55621 gctgccaaaa agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat
    55681 gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcac
    55741 tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa
    55801 gccgaccaca tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac
    55861 ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt
    55921 actaaaaacg cgatccgcca ggcatttaac aaaccgggtg agctgaatat tgatcgtgtt
    55981 aatgcccagc aggcgcgtcg ctttatggac cgcgtggttg ggtatatggt ttcgccgctg
    56041 ctatggaaaa agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggttcgc
    56101 ctggtggtcg agcgtgagcg tgaaattaaa gcgttcgtgc cggaagagtt ctgggaagtc
    56161 gatgccagca cgaccacgcc atctggtgaa gcgttggcgt tgcaggtgac tcatcagaac
    56221 gacaaaccgt tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa
    56281 aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct
    56341 ccttttatta cctctacgct gcaacaagct gccagcaccc gtcttggatt tggcgtgaaa
    56401 aaaaccatga tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc
    56461 gactccacta acctgagtca ggacgcggta aatatggttc gcggttatat cagcgataat
    56521 tttggtaaga aatatctgcc ggaaagtccg aatcagtacg ccagcaaaga aaactcacag
    56581 gaagcgcatg aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat
    56641 atggaagcag atgcgcagaa actgtaccag ttaatctggc gtcagttcgt agcctgccag
    56701 atgaccccag cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg
    56761 aaagcacgcg gtcgtatttt gcgttttgat ggctggacaa aagtgatgcc tgcgttgcgt
    56821 aaaggcgatg aagatcgcat cttaccagca gttaataaag gcgatgctct gacgctcgtt
    56881 gaacttacac cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg
    56941 gttaaagagc tggaaaaacg tggtatcggt cgtccgtcta cctatgcgtc gatcatttcg
    57001 accattcagg atcgtggcta cgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg
    57061 ggcgaaatcg tcaccgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt
    57121 accgcgcaga tggaaaacag cctcgaccaa gtggcaaatc acgaagcaga gtggaaagct
    57181 gtactggatc acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg
    57241 gaagagggtg gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt
    57301 ggtcgcaaaa tggggattcg cacagcgagc accggggtat tccttggctg ttctggctat
    57361 gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg
    57421 ctgaacgtgc tggaaggcga agatgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc
    57481 ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc
    57541 tgtggtaata acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa
    57601 ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg
    57661 gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt
    57721 ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg
    57781 tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct
    57841 gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc
    57901 ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg
    57961 gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg
    58021 gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa
    58081 ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc
    58141 cttttttatt gcatattatt ttttcttcac acctatacac taaggctata aatgatatag
    58201 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
    58261 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
    58321 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
    58381 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
    58441 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
    58501 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
    58561 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
    58621 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
    58681 accagggctc gccgacacaa attgctgatg ccgtctctaa aggcaatgct gatttcgcta
    58741 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
    58801 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
    58861 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
    58921 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg
    58981 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
    59041 cggtggatcc ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc
    59101 acagtacaac caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt
    59161 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
    59221 gctctaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
    59281 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
    59341 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
    59401 aaagtagtgt ctaattaccc gcctcgcaaa ctccagtttt caaatatcta tttcaggtca
    59461 aaagattgaa ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt
    59521 ctttaggatt tgtctcatcg tatgattaat tacaccaatc gttcaacgtc ttgctgataa
    59581 gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgcgatc
    59641 ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg
    59701 ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga
    59761 gtcgtcacca ttatgccagc atagtgacaa tgaaacagaa acgaaacggg aatgttccgt
    59821 cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc
    59881 tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt
    59941 aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa
    60001 ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg
    60061 ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg
    60121 accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt
    60181 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
    60241 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
    60301 cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
    60361 tgctaaatca ctgggcgata tcacccgtct gcccaagtca ctcaaagttt tgctcgaaaa
    60421 cctgctgcgc tggcaggatg gtaactcggt taccgaagag gatatccacg cgctggcagg
    60481 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat
    60541 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
    60601 acgcctcggc ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga
    60661 ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct
    60721 ggaaatggag cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag
    60781 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
    60841 cggcaaagca gtgtggagtg aattgcagga cggtgaatgg attgcttatc cggatacact
    60901 cgttggtact gactcgcaca ccactatgat caacggcctt ggcgtgctgg ggtggggcgt
    60961 gggtgggatc gaagcagaag ccgcaatgtt aggccagccg gtttccatgc ttatcccgga
    61021 cgtagtgggc tttaaactta ccggtaaatt acgtgaaggt attaccgcca cagacctggt
    61081 tctcactgtt acccaaatgc tgcgcaaaca tggcgtggtg gggaaattcg tcgaatttta
    61141 tggt
//
LOCUS       DAFATA010000016        59292 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFATA010000016 DAFATA010000000
VERSION     DAFATA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.016"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..231
                     /locus_tag="LGJ20_001370"
     CDS             <1..231
                     /locus_tag="LGJ20_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBK2385461.1"
                     /translation="GYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVL
                     NKAGQIQWAKDGSLTPEEVDHVIALAQKLINE"
     gene            262..1332
                     /gene="lptF"
                     /locus_tag="LGJ20_001371"
     CDS             262..1332
                     /gene="lptF"
                     /locus_tag="LGJ20_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBK2385462.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            1329..2408
                     /gene="lptG"
                     /locus_tag="LGJ20_001372"
     CDS             1329..2408
                     /gene="lptG"
                     /locus_tag="LGJ20_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBK2385463.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     EILDNLAWQSTFTSMNLEELSMPGNSFSVRQLNHYINYLQETGQPSSEYRLALWEKLG
                     HPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VAALGLPMAFVLAALILVYLFDRQH"
     gene            complement(2443..3615)
                     /locus_tag="LGJ20_001373"
     CDS             complement(2443..3615)
                     /locus_tag="LGJ20_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK2385464.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(3615..3863)
                     /locus_tag="LGJ20_001374"
     CDS             complement(3615..3863)
                     /locus_tag="LGJ20_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK2385465.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(3895..4809)
                     /locus_tag="LGJ20_001375"
     CDS             complement(3895..4809)
                     /locus_tag="LGJ20_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK2385466.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(4806..6536)
                     /locus_tag="LGJ20_001376"
     CDS             complement(4806..6536)
                     /locus_tag="LGJ20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBK2385467.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
     gene            6898..8040
                     /locus_tag="LGJ20_001377"
     CDS             6898..8040
                     /locus_tag="LGJ20_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2385468.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(8047..8811)
                     /gene="glcC"
                     /locus_tag="LGJ20_001378"
     CDS             complement(8047..8811)
                     /gene="glcC"
                     /locus_tag="LGJ20_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBK2385469.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            9062..10561
                     /gene="glcD"
                     /locus_tag="LGJ20_001379"
     CDS             9062..10561
                     /gene="glcD"
                     /locus_tag="LGJ20_001379"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBK2385470.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            10561..11613
                     /gene="glcE"
                     /locus_tag="LGJ20_001380"
     CDS             10561..11613
                     /gene="glcE"
                     /locus_tag="LGJ20_001380"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBK2385471.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            11624..12847
                     /gene="glcF"
                     /locus_tag="LGJ20_001381"
     CDS             11624..12847
                     /gene="glcF"
                     /locus_tag="LGJ20_001381"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBK2385472.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLERARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            12852..13256
                     /locus_tag="LGJ20_001382"
     CDS             12852..13256
                     /locus_tag="LGJ20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBK2385473.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            13278..15449
                     /gene="glcB"
                     /locus_tag="LGJ20_001383"
     CDS             13278..15449
                     /gene="glcB"
                     /locus_tag="LGJ20_001383"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBK2385474.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQLERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            15805..17487
                     /gene="glcA"
                     /locus_tag="LGJ20_001384"
     CDS             15805..17487
                     /gene="glcA"
                     /locus_tag="LGJ20_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBK2385475.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            17972..22540
                     /gene="sslE"
                     /locus_tag="LGJ20_001385"
     CDS             17972..22540
                     /gene="sslE"
                     /locus_tag="LGJ20_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HBK2385476.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRV
                     TGDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDA
                     QELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFK
                     KLVNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIIL
                     SEGRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKS
                     TIALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLS
                     NGATLDEGEQVVNLPNAFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQ
                     IQGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARND
                     KNYWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVI
                     GNPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKA
                     SMTVGTNLDTVYFKRHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNG
                     FEYVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESA
                     SGFVRLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTID
                     SKTGEVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKE
                     KIFAAFPGLKECTNPAYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQ
                     AADLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKND
                     ELGFKTFTEFLNCYANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNY
                     MEKPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQST
                     GLWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTV
                     KFKVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAF
                     VYTTPKKNLNASNYTGGLEQFANDLDTFARSMNDFYGRDETSGKHRMFTYKALTGHKH
                     RFTNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPG
                     ATEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQL
                     KEWAEKNFDIKKWYPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGDKTFGGKNY
                     CAESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYN
                     TLASLNLPKPKQGPETINKVTEYSMPAE"
     gene            22680..23489
                     /gene="pppA"
                     /locus_tag="LGJ20_001386"
     CDS             22680..23489
                     /gene="pppA"
                     /locus_tag="LGJ20_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HBK2385477.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVIIKKGSTTLPFGPCLSLGGIATLYLQALF"
     gene            23555..23965
                     /gene="gspS2"
                     /locus_tag="LGJ20_001387"
     CDS             23555..23965
                     /gene="gspS2"
                     /locus_tag="LGJ20_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HBK2385478.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLISYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            23983..24942
                     /gene="gspC"
                     /locus_tag="LGJ20_001388"
     CDS             23983..24942
                     /gene="gspC"
                     /locus_tag="LGJ20_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBK2385479.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            24972..27032
                     /gene="gspD"
                     /locus_tag="LGJ20_001389"
     CDS             24972..27032
                     /gene="gspD"
                     /locus_tag="LGJ20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBK2385480.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            27032..28525
                     /gene="gspE"
                     /locus_tag="LGJ20_001390"
     CDS             27032..28525
                     /gene="gspE"
                     /locus_tag="LGJ20_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBK2385481.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDGAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            28525..29748
                     /gene="gspF"
                     /locus_tag="LGJ20_001391"
     CDS             28525..29748
                     /gene="gspF"
                     /locus_tag="LGJ20_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBK2385482.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            29765..30220
                     /gene="gspG"
                     /locus_tag="LGJ20_001392"
     CDS             29765..30220
                     /gene="gspG"
                     /locus_tag="LGJ20_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBK2385483.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            30224..30787
                     /gene="gspH"
                     /locus_tag="LGJ20_001393"
     CDS             30224..30787
                     /gene="gspH"
                     /locus_tag="LGJ20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBK2385484.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPAAPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            30784..31155
                     /gene="gspI"
                     /locus_tag="LGJ20_001394"
     CDS             30784..31155
                     /gene="gspI"
                     /locus_tag="LGJ20_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBK2385485.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            31152..31757
                     /gene="gspJ"
                     /locus_tag="LGJ20_001395"
     CDS             31152..31757
                     /gene="gspJ"
                     /locus_tag="LGJ20_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBK2385486.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            31754..32731
                     /gene="gspK"
                     /locus_tag="LGJ20_001396"
     CDS             31754..32731
                     /gene="gspK"
                     /locus_tag="LGJ20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBK2385487.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLTQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDDGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVLLEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            32728..33906
                     /gene="gspL"
                     /locus_tag="LGJ20_001397"
     CDS             32728..33906
                     /gene="gspL"
                     /locus_tag="LGJ20_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBK2385488.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            33908..34444
                     /gene="yghD"
                     /locus_tag="LGJ20_001398"
     CDS             33908..34444
                     /gene="yghD"
                     /locus_tag="LGJ20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HBK2385489.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(34725..35567)
                     /locus_tag="LGJ20_001399"
     CDS             complement(34725..35567)
                     /locus_tag="LGJ20_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBK2385490.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAHARDTWYRSLEYDNFPEISKANILSTFEQLHRSKDEVFERGVINVFRGLN
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLISGMQADRLADLERMLHLLSGKPIPNNR
                     GNITINLDDHIRSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARHFPS
                     VLSA"
     gene            complement(35652..35849)
                     /locus_tag="LGJ20_001400"
     CDS             complement(35652..35849)
                     /locus_tag="LGJ20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385491.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRILRQLQFLQQNR"
     gene            complement(35869..36360)
                     /locus_tag="LGJ20_001401"
     CDS             complement(35869..36360)
                     /locus_tag="LGJ20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385492.1"
                     /translation="MMKLALTLEADSVNVRALNMGRIVVDIDGVNLAELINNVAENGY
                     SLRVVEEFDQQSTCTPPPFTALAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGLPT
                     FDW"
     gene            complement(36357..36731)
                     /gene="cbtA"
                     /locus_tag="LGJ20_001402"
     CDS             complement(36357..36731)
                     /gene="cbtA"
                     /locus_tag="LGJ20_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HBK2385493.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAK"
     gene            complement(36821..37189)
                     /locus_tag="LGJ20_001403"
     CDS             complement(36821..37189)
                     /locus_tag="LGJ20_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBK2385494.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGSRLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELILTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            complement(37352..37573)
                     /locus_tag="LGJ20_001404"
     CDS             complement(37352..37573)
                     /locus_tag="LGJ20_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBK2385495.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(37642..38118)
                     /locus_tag="LGJ20_001405"
     CDS             complement(37642..38118)
                     /locus_tag="LGJ20_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBK2385496.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(38134..38613)
                     /locus_tag="LGJ20_001406"
     CDS             complement(38134..38613)
                     /locus_tag="LGJ20_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBK2385497.1"
                     /translation="MKIVSQNTTTLSSATAPGNNQPQLVATPVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGKTWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACHTECYAMTVHYYRLRDYALQHPECNAIMRIID"
     gene            complement(38876..39694)
                     /locus_tag="LGJ20_001407"
     CDS             complement(38876..39694)
                     /locus_tag="LGJ20_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBK2385498.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDRHTSRSERY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFICQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGNEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(39785..39994)
                     /locus_tag="LGJ20_001408"
                     /pseudo
     CDS             complement(39785..39994)
                     /locus_tag="LGJ20_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(40095..40550)
                     /locus_tag="LGJ20_001409"
     CDS             complement(40095..40550)
                     /locus_tag="LGJ20_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385499.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRATDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(40626..43142)
                     /locus_tag="LGJ20_001410"
     CDS             complement(40626..43142)
                     /locus_tag="LGJ20_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385500.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLA
                     VASSSVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            complement(43263..46382)
                     /gene="ag43"
                     /locus_tag="LGJ20_001411"
     CDS             complement(43263..46382)
                     /gene="ag43"
                     /locus_tag="LGJ20_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBK2385501.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNMVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLMRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASSD
                     NNDFRVRGRGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHGS
                     AQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRVD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGYN
                     GQATLNVTF"
     gene            complement(46754..47626)
                     /locus_tag="LGJ20_001412"
     CDS             complement(46754..47626)
                     /locus_tag="LGJ20_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBK2385502.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            48857..50341
                     /locus_tag="LGJ20_001413"
     CDS             48857..50341
                     /locus_tag="LGJ20_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBK2385503.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(50663..51265)
                     /locus_tag="LGJ20_001414"
     CDS             complement(50663..51265)
                     /locus_tag="LGJ20_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385504.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(51352..51558)
                     /locus_tag="LGJ20_001415"
     CDS             complement(51352..51558)
                     /locus_tag="LGJ20_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBK2385505.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            52296..52439
                     /locus_tag="LGJ20_001416"
                     /pseudo
     CDS             52296..52439
                     /locus_tag="LGJ20_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(52780..52977)
                     /locus_tag="LGJ20_001417"
     CDS             complement(52780..52977)
                     /locus_tag="LGJ20_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385506.1"
                     /translation="MVIRKKKCRDCGKTITHNTVCCPYCGSVDPFGYYRNTDRLLCLL
                     ALLLVLILVTVSGVSVFVLLP"
     gene            53177..53746
                     /locus_tag="LGJ20_001418"
     CDS             53177..53746
                     /locus_tag="LGJ20_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBK2385507.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRDPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERDDDTGFQ
                     QVLARADYLAKESTKAYGTGERNFGCSRS"
     gene            54005..54388
                     /locus_tag="LGJ20_001419"
     CDS             54005..54388
                     /locus_tag="LGJ20_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385508.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEVVAG"
     gene            54385..54810
                     /locus_tag="LGJ20_001420"
     CDS             54385..54810
                     /locus_tag="LGJ20_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385509.1"
                     /translation="MNGIKMGLGITPGEHIISANSALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLYQDCGGAST"
     gene            55059..55256
                     /locus_tag="LGJ20_001421"
     CDS             55059..55256
                     /locus_tag="LGJ20_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBK2385510.1"
                     /translation="MHQDDFCIVNPGGCIIHFPANGRYIRDIHDPDFSEQYQRLLERL
                     DYLKKPGTKASGQRNSGYSGF"
     gene            55284..55568
                     /locus_tag="LGJ20_001422"
     CDS             55284..55568
                     /locus_tag="LGJ20_001422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385511.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPPPFILSLEQGGCG"
     gene            55561..55851
                     /locus_tag="LGJ20_001423"
     CDS             55561..55851
                     /locus_tag="LGJ20_001423"
                     /inference="COORDINATES: protein motif:HMM:NF023154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3296 domain-containing protein"
                     /protein_id="HBK2385512.1"
                     /translation="MANDTTPYLMVLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDN
                     SITHLVVYPEVLSLVFDAANTYNAEPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            56201..56698
                     /locus_tag="LGJ20_001424"
     CDS             56201..56698
                     /locus_tag="LGJ20_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385513.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCTSFYELITVRNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSVCHPRASV
                     TVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLMPRGI
                     ATIIR"
     gene            56787..57410
                     /locus_tag="LGJ20_001425"
     CDS             56787..57410
                     /locus_tag="LGJ20_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK2385514.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRAPQQIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQITREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKIPAYHL"
     gene            57506..57739
                     /locus_tag="LGJ20_001426"
     CDS             57506..57739
                     /locus_tag="LGJ20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385515.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLADNRNRLAEVVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(57792..57983)
                     /locus_tag="LGJ20_001427"
     CDS             complement(57792..57983)
                     /locus_tag="LGJ20_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBK2385516.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTVSSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            58659..>59292
                     /locus_tag="LGJ20_001428"
     CDS             58659..>59292
                     /locus_tag="LGJ20_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HBK2385517.1"
                     /translation="MSVLLSPLSLQAADIRRSGEDTFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLENMQRLPGVQADMEIVPGEQPGESDILITRKQ"
BASE COUNT        14091 a        14838 c        16088 g        14275 t
ORIGIN      
        1 ggctattttg tcgttggcaa aatcgaaaaa aataaacgcc gctatccgtg ggcgcaattc
       61 gttattgacg gcaatggaca gggacgcgtt gcctggcgct tgccggagca gagttcaacc
      121 attctggtgc tgaataaagc cgggcaaatt cagtgggcaa aggacggcag cctgacgccg
      181 gaagaggtag atcatgtcat tgcgttggcg cagaaactga tcaatgaata aaaaactcaa
      241 aggattgagg gcgttagtta gatgaagctg gttgaacact acatcatgcg tggtacgcgc
      301 cgtctggtgc tgattatcgt cggttttctg atctttattt tcgccagcta ctccgcacag
      361 cgttatctga ccgaagcggc aaatgggaca ttagctcttg atgttgtgct ggatatcgtt
      421 ttctacaaag tactgattgc actggagatg ttgttacctg ttggtctgta tgtgtcagtt
      481 ggcgtgacgc tagggcagat gtataccgac tcggaaatta ccgctatctc tgcggcgggg
      541 ggcagtccgg gacgcttgta caaagccgtt ctttatctgg cgataccgct aagtattttt
      601 gtcacccttc tgtcgatgta tggtcgtccg tgggcttatg cgcagattta tcaactggag
      661 cagcagtcgc agtcggagct ggatgttcgc cagttgcggg caaagaaatt taacactaac
      721 gataacggac gaatgatcct ttcgcagacg gttgatcagg ataataatcg cctgactgac
      781 gcgctgattt atacttctac tgccaatcga acccgtattt tccgcgcccg ttcggttgat
      841 gtggttgacc catcacccga gaaaccgacc gttatgttgc acaacggcac tgcctatctt
      901 ctcgatcatc aggggcgtga cgacaacgaa cagatctacc gtaatctgca attacatctg
      961 aatccgctgg atcaaagccc taacgtcaaa cgcaaagcaa aatcggtcac ggagctggcg
     1021 cgctccgcct ttcctgccga tcatgccgaa ctgcaatggc gacaaagccg tggcctgacc
     1081 gcattgttga tggcgctgct ggccatttca ttaagtcggg taaaaccgcg gcaagggcga
     1141 ttttcaacgt tattgccact gacgttgctg tttattgcca ttttttatgg cggcgacgtc
     1201 tgccgtacgc tggtggctaa cggcgcgatt cccctcattc ctggtttgtg gttagtaccc
     1261 ggactcatgc taatgggcct gctgatgctg gtcgcacgcg acttctcttt gctgcagaaa
     1321 ttttcccgat gaatgttttc agtcgctatt taatccgtca tctctttctc ggttttgccg
     1381 ccgccgcagg gctattgctg ccgcttttta ccaccttcaa cctgattaac gaactggatg
     1441 atgtcagccc gggcggttat cgctggactc aggcggtgct ggtggtgcta atgaccttac
     1501 cgcgcacact ggtcgaactt tcgccgttta tcgcgttatt gggtggtatt gttggactgg
     1561 ggcagttatc gaaaaacagt gagcttaccg ccattcgcag cacggggttt tctatcttcc
     1621 gtattgcact ggtggcgctg gttgccggga tattgtggac tgtttcgtta ggcgcgatag
     1681 atgagtgggt ggcgtcgcca ttgcagcaac aggcgttgca aatcaaatcg accgccaccg
     1741 cgttggggga ggacgatgat attaccggca atatgctgtg ggccaggcgc ggtaatgaat
     1801 ttgtgacggt gaaatcgctg aatgaacagg gccagcctgt gggcgtggag atatttcatt
     1861 atcgcgacga tctctcgctc gaatcctaca tttatgcacg cagtgccacc attgaagacg
     1921 acaaaacgtg gattctgcat ggtgtgaatc ataaaaaatg gctgaatggc aaagaaacgc
     1981 tggaaatatt agataatctt gcctggcaat cgacattcac cagtatgaat cttgaagaac
     2041 tgtcgatgcc gggcaatagc ttttccgttc gtcagctcaa ccattacatc aattacttgc
     2101 aggaaaccgg acaaccgagc agtgagtatc gcctggcatt gtgggaaaag ctggggcatc
     2161 ccattttaac cctggcgatg attttgctgg cagtgccttt tacctttagc gccccgcgct
     2221 cgccggggat gggcagccgt cttgctgtag gcgtcatcgt tggcttactt acctggataa
     2281 gctaccaaat aatggtcaat ctgggattat tatttgcgct gagtgcgccc gttgctgcac
     2341 tcggtttacc tatggcattt gtgctggcgg cgttgattct ggtgtatcta tttgataggc
     2401 aacattaaac ccgccgtggc gcaacaccac ggcgggctca cattactgct tcagagtagc
     2461 aaatgcctga atgatggcat cgatttgctc acgactgtgg gcagcattta cgctacagcg
     2521 cagcagcgtg atgcctgcag gcgcggcagg cggcaggata agattcacat acacgcccag
     2581 cgagataagc tcgcgccaga tgcgtaaacc ttcctctttc gaaccgatta ttactggcac
     2641 taccgggcta atatgtgaac ttaactcata acccagtttt tgcagaccgt catacagatg
     2701 gttggcgtta tccatcagct tttgccgcag ctctggttgt gtggcaatag ttcttaacga
     2761 cgagcgcacg gtcgcaatgc aggacggaga aggagaggcg gtaaaaatat acgggcggct
     2821 gccgtagcgc agcacttcca tcgcttccga accgacagca aaaccgccaa tcgaggccag
     2881 acttttactg aaagtaccaa cgataatatc cacatcatct tcaacgccga cagcttcagc
     2941 cagtccgcgc cccgttgcgc ctaacacgcc aaacgaatgc gcttcatcga caatcaaata
     3001 accaccgaga cgacgtttga tatcaacaat ttccgccagc ggcgcaacgt caccgagcat
     3061 gctgtagatg ccctcaacga tgataatcgc ctctttcgcc cgttcaccga ggcgaaccat
     3121 gcgccgttcc agatctttag catcgttatg gcggaaacga ataatttccg cgccaccgag
     3181 agaacaggcg tcataaatac tggcgtggct gtcggcatcc agtaacacca cggcgttatg
     3241 gtcggcaagt gcgctgatga cgcctaaatt agcggtataa ccggttgaaa aaacgatagc
     3301 ggtagggcga ttgaaaaact cggcaatctc tttctctaac gccaggtgcg gcgcataact
     3361 gccgtttgcc atacgagaac cggtggtgcc ggtaccttgg gccgctaacg cggcctgacc
     3421 ttcgctgatg gcatcatgat taaaggttaa accgagataa ttattggtcc cggccagaat
     3481 cactttttta ttgccgatcc ggccttcggt ggcggaatac acttcatcaa tacaggtgcc
     3541 aaacggtgtt aacccgttgt cactaaattg cagtcgctcg ccagcaaggc gcgcatattt
     3601 atcgtatagc cccattattt attctccagc catggaagta gagtttccat taattgcgca
     3661 ggggttttga catccagcag aatattaata ggaatggaaa tatcgaatct gtcttccagc
     3721 atcattaaga gatccatcac tttgatcgac tccaggccaa gatcgttgac gagatcgctg
     3781 tcgggcttaa tttccacacc gttttcgacc aaatcctgta aacaggaaag gatgtaatcc
     3841 attactattt cacgatttac catagaaacg ttaacacacc tttaaacaag acaatcaaaa
     3901 tagcccgttg cgtaatgcgt gttccaggct aatcccggga aaccaattaa tatcttcaga
     3961 aatacggtta ttacttgccg accagtcggc gtgggttaat tcacgaattt tcgagcgggt
     4021 cagcataggt tctttacctg ccaggcgact caacgcggta ctgatatccg caaggcaggt
     4081 gagtaccggc agaggaatac caaccattcg cacggaaccg caacggacgt tggcggcaag
     4141 ttgctgtatg cgttgccagt catagccgcc agcgacgcca tcgcataatt cataggtttg
     4201 cgtctgtaca gtttcggcgc ttaaccactg acccactgct tgcgcaaaat cggtgacgtg
     4261 caggaaagag agctgtgtat ctggtgcacc aagtcgtggc agcaggccgc gcagcatcca
     4321 gtcaaacagc ggttttaact ctttatcgcc gggaccatac acggctgtcg ggcgaaaaac
     4381 gcccagcgta atttcgtcag ccattgcagt cagccgttgt tcggcgatgt gtttggaatt
     4441 tgcgtaccag gagagctcgg gatggcgcgc cgccagcgaa gagataaaca gaaaacgttg
     4501 gcaaaagcag ctctcttttg ctgcttgcat caggcgcagg ctgccgtcaa cgttacagcg
     4561 ggtgaaaatc tcttctttgt gcccgcgcac ttgcccggcg caatggacca ctgcgctggc
     4621 tccggcaacc agcttgctaa gtgaatgagt atcttccagc gaaccgcgca cccaggtgag
     4681 attatcattg acgtgagcgc gggcagtacg cgtcaatgcg cgaacgtgaa agccgcgggc
     4741 gagcaggtta tcgataatat atttaccgat aaacccggta gcgcccgtca ccgcgacagt
     4801 ttgattcatg ccagggattc ctgcacatga aggcttgcag cataagcctt ctgataacgt
     4861 tttttcgctt ccgcacgggc aggcttgccg gaggatgttc ggggaatact gtggggcggc
     4921 aacagatcga tagccgctgt tacgccaaat tcgctttgga tccgtgctgc cagcgcgtgg
     4981 ataagctgcc cgcgacgttc ttcgtcgctg atccgacact ggatctgcaa aatgattttt
     5041 tcctgggcgg taacaaaagc aatcgcatcg ccagaatgaa tttccggttc ttgttccgca
     5101 atatattcaa tatcctgcgg ccagatatta cggccacgaa taataatcag atctttaata
     5161 cgtccggtga catacagata accgtccagc agataaccga ggtcgccggt gtctaaccag
     5221 cccgtcgcgg caatctcgtc ttgcgaaacc tggtcgccaa agtaaccgct catcaggctg
     5281 gggccggaga tgcaaatatg gcctaccaca cgttccgcga ccggcatacc tgcttcattg
     5341 cggatttcaa tgccatgttc cggcaacgct ttgccgcagt tgacgaaagt cgatacggcg
     5401 cgtgtttcag cacccggcgc gacggctttg ccctgatatt ctaggatgtc gcgatccact
     5461 tcgttaacca caaccccaga gccttcatca gagaagctga cagccagcgc attttctgcc
     5521 agtccgtagc acggcatgaa cgttttatcg tcaaagttaa cctgacggaa acattcagcg
     5581 aattgatgga gttgttctgc ggagatcggc tcagcaccaa taccagcgac gcgccagcag
     5641 gaaagatcca gttcagcgag atctttttca ttcacgcggc gttggcacaa ttcatagcca
     5701 aacggcggcg caacggaaac ggtgccgcga tttttactga tcaatttaag ccattgcaaa
     5761 ggacgcatgg caaaatcctg agtgcgcaaa taatctactg aaagttgcgt ggcgacgggg
     5821 gtcaggagaa agccgaccag tcccatatca tggtagaaag gcagccagga gacgcagcgg
     5881 tcgccagggc gtaatttaat gccatcgtgg cttatagcac gtagattagc catcacttcg
     5941 cgatgggtga taatgacgcc acggggaaaa cgggtgctgc cggaggtgta ctggaggtag
     6001 gcgatatcgt tcggaactgg acgctggagc gcaacatcgg cttccggtaa tgccttaaac
     6061 caggcgtggc ttaaaacatg taattcgggg ttgttatgcg tcgcggcatt gaccagtggc
     6121 aaccactcat caccagtgat aatggctgcg ggctggcaac ttgccagtaa accctggagt
     6181 ttggcgctcc aggaatcccg ctgaccgacg cccattggaa tcgccaacgg gacggcgact
     6241 aagccagcat actgacaggc aaaaaaagcc tctacaaacc cgctactcgt ttcggcaatc
     6301 agcgccacgc gttcgccttt tttcaggttc agcgataaca accgcttcgc accagcttcg
     6361 gcacgcgttt ttaacgtctg atattccagt tgatcttcaa gttggcaacg tctgtcataa
     6421 aaattcattc cggcgctact cagagcggcg tagtccaaag catcaaccag cgttggaaaa
     6481 tcggcatagc gcataggtag ggaatgcgta aagattttat tagacatata caccacgaaa
     6541 aaattggata tcaaaaataa aatgacaata atgcggtgga ttatataggc ataaaagata
     6601 aatgagatag tattttcatc ggtgtgaaaa tgaacattga tttaaataat gagtttaagt
     6661 tttttgtttt tattgtgaat gatatttaat ttatgaatat attaagaaga agccttgtcc
     6721 ggtcgcctta aatcgacaaa aggcattgag gcgcttctgt gtagtctcta accttatcat
     6781 aatcattaat aaatactatt tattctttgt ttgtattcat tgggttgatt attaaggtgt
     6841 cgttcgttgc tgttgcaacc tgtttttcac cgttaattat ttgttatcca cagagatatg
     6901 gaatgcgatc tgctgatgat taaaattgaa aaagtaatta ataaaaatga ccttaaggca
     6961 tttatcgctt ttccgtcatc actttatccc gacgatccaa actggatacc ccctttattc
     7021 atagaacgca acgagcattt gtctgcgaaa aatccgggga cggaccatat catctggcag
     7081 gcgtgggtgg caaaaaaagc ggggcagata gttgggcgta ttaccgcgca aatagatacc
     7141 ttgcaccgtg agcgttacgg tgaagatacc ggtcatttcg gcatgattga cgccattgat
     7201 gatccgcagg tttttgccgc gttgtttggc gcagcggaag cgtggttgaa gtcacaaggt
     7261 gcaagtaaga tcagcgggcc tttcagcctg aatatcaatc aggaaagcgg attactgatt
     7321 gaaggttttg acacaccacc ctgtgcgatg atgccacacg gcaaaccgtg gtatgccgcg
     7381 catattgaac aactgggtta tcacaaaggc attgatttac tggcgtggtg gatgcagcga
     7441 accgatctca ctttctctcc ggcgctaaaa aaactgatgg atcaggtgcg caaaaaggtg
     7501 accattcgct gcatcaatcg tcagcggttt gccgaagaga tgcagatcct gcgtgagatt
     7561 ttcaactctg gctggcagca caactgggga tttgtgccgt ttaccgaaca tgaattcgcg
     7621 accatgggcg atcaacttaa atatctggtg ccggacgata tgatctacat cgctgagatt
     7681 gattctgcac cctgcgcgtt tattgtcggc ttgccgaaca tcaacgaggc gattgccgat
     7741 ctgaacggat cgctctttcc cttcggctgg gcaaaattgc tgtggcgttt gaaagtcagc
     7801 ggtgtgcgaa ctgcgcgagt accgctgatg ggcgtgcgcg acgagtatca gttcagccgc
     7861 atcggcccgg tgattgcgct gttattgatt gaagccttac gcgatccgtt tgcccgccgg
     7921 aagatcgatg cgctggagat gtcatggatc ctcgaaacca atacaggcat gaataacatg
     7981 ctggaacgca ttggggcgga gccgtacaag cgttaccgat tgtatgaaaa gcagatttaa
     8041 taccagctaa ctcaggttca tctccagcgg caccgccgag cgaatcaaat ggtggccttc
     8101 cagctcgata tcgtggagat tctttttcac ggtccgcaca tgatcgcgtg ctgcgcgctg
     8161 ggcgacgtgc ggcagccgct gcaacaccgc gttgtagatc ctcgcatgct ggcgatcgat
     8221 ctgctttttt tgctgtggtc gatggtagag attatttacg ctggcaaaca ctgaattaaa
     8281 catcagatcg gtcaatgatt gcagcgtaaa caccagcacc tgattgtgag aagcctgaca
     8341 aatggcgaga tggaaagcgt gatccaactg cgcatgttcg atcagcgaaa tctctttgtt
     8401 gttctcactg gcggcgagca ttttttcata acagcgggtt atcacaacaa aatcagcctg
     8461 cgttcccagc gttgccgcca gccttgccga ttcgccctcc agtaatgcgc gaacgtcgag
     8521 cagatcgtac agcgttcgcg gctgcgtact gaacagatgg atcagcgggc tggtgtcctg
     8581 cacccgatta agccgtgcga cacgagaatc gcgaccctgc gccgtttcaa taatcccgcg
     8641 cccgcgcagc acggtcagcc cttcacgcag tgcggagcgt gagaagccga gcttttcaca
     8701 cagtcgacgt tccgagggaa gtggctgacc gaccttcagt acgccgtcga taattaaccg
     8761 ttcgatactc tctgcaacca cttcgcaaat agggcgacgt tcatctttca ttcccggacc
     8821 tcgtgcacag gtaggaccaa tttttctgtt cttaactcgc aaaacacgca catcacgtaa
     8881 gtgattatgt tacatcaatt taacattgag ttaaccaagg caaggtcaca gagctggaaa
     8941 aaaaatggtc tgaccggtag ctaaagagat agacgaaaac gaaaagcccg cttaataact
     9001 gttcacagaa gcagcgcgca aaaatcagct gccacacaac acaacaaagc gaagcctact
     9061 catgagcatc ttgtacgaag agcgtcttga tggcgcttta cccgatgtcg accgcacatc
     9121 ggtactgatg gcactgcgtg agcatgtccc tggacttgag atcctgcata ccgatgagga
     9181 gatcattcct tacgagtgtg acgggttgag cgcgtatcgc acgcgtccat tactggttgt
     9241 tctgcctaag caaatggaac aggtgacagc gattcttgct gtctgccatc gcctgcgtgt
     9301 accggtggtg acccgtggtg caggcaccgg gctttctggt ggcgcgctgc cgctggaaaa
     9361 aggtgtgttg ttggtgatgg cgcgctttaa agagatcctc gacattaacc ccgttggtcg
     9421 ccgcgcgcgc gtgcagccag gcgtgcgtaa cctggcgatc tcccaggccg ttgcaccgca
     9481 taatctctac tacgcaccgg acccttcctc acaaatcgcc tgttccattg gcggcaacgt
     9541 ggcagaaaat gccggcggcg tccactgcct gaaatatggt ctgaccgtac ataacctgct
     9601 gaaaattgaa gtgcaaacgc tggacggcga ggcgctgacg ctgggatcgg acgcgctgga
     9661 ttcacctggt tttgacctgc tggcgctgtt caccggatcg gaaggtatgc tcggcgtgac
     9721 caccgaagtg acggtaaaac tgctgccgaa gccgcccgtg gcgcgggttc tgttagccag
     9781 ctttgactcg gtagaaaaag ccggacttgc agttggtgac atcatcgcca atggcattat
     9841 ccccggcggg ctggagatga tggataacct gtcgatccgc gcggcggaag attttattca
     9901 tgccggttat cccgtcgacg ccgaagcaat tttgttatgc gagctggacg gcgtggagtc
     9961 tgacgtacag gaagactgcg agcgggttaa cgacatcttg ttgaaagcgg gcgcgactga
    10021 cgtccgtctg gcacaggacg aagcagagcg cgtacgtttc tgggccggtc gcaaaaatgc
    10081 gttcccggcg gtaggacgta tctccccgga ttactactgc atggatggca ccatcccgcg
    10141 tcgcgccctg cctggcgtac tggaaggcat tgcccgttta tcgcagcaat atgatttacg
    10201 tgttgccaac gtctttcatg ccggagatgg caacatgcac ccgttaatcc ttttcgatgc
    10261 caacgaaccc ggtgaatttg cccgcgcgga agagctgggt gggaagatcc tcgaactctg
    10321 cgttgaagtt ggcggcagca tcagtgggga acatggcatc gggcgagaaa aaatcaatca
    10381 aatgtgcgcc cagttcaaca gcgatgaaat cacgaccttc catgcggtca aggcggcgtt
    10441 tgaccccgat ggtttgctga accctgggaa aaacattccc acgctacacc gctgtgctga
    10501 atttggtgcc atgcatgtgc atcacggtca tttacctttc cctgaactgg agcgtttctg
    10561 atgctacgcg agtgtgatta cagccaggcg ctgctggagc aggtgaatca ggcaattagc
    10621 gataaaacgc cgctggtgat tcagggcagc aatagcaaag cctttttagg tcgccctgtc
    10681 accgggcaaa cgctggatgt gcgttgtcat cgcggcattg ttaattacga cccgaccgag
    10741 ctggtgataa ccgcgcgtgc cggaacgccg ctggtggcga ttgaagcggc gctggagagc
    10801 gccgggcaaa tgcttccctg cgagccgccg cattatggtg aagaagccac ctggggtggg
    10861 atggtcgcct gcgggctggc ggggccgcgt cgcccgtgga gcggttcggt ccgcgatttt
    10921 gtcctcggca cgcgcatcat taccggcgct ggaaaacatc tgcgttttgg tggcgaagtg
    10981 atgaaaaacg ttgccggata cgatctctca cggttaatgg ccggaagcta cggttgtctt
    11041 ggcgtgctca ctgaaatctc gatgaaagtg ttaccgcgac cgcgcgcctc cctgagcctg
    11101 cgtcgggaaa tcagcctgca agaagccatg aacgaaatcg cccagtggca actccagccg
    11161 ctacccatta gtggcttatg ttacttcgac aatgcgttgt ggatccgcct tgagggcggc
    11221 gaaggatcgg taaaagcggc gcgtgaactg ctggggggcg aagaggttgc gggtcagttc
    11281 tggcagcaat tgcgtgaaca acaactgccg ttcttctcgt taccaggtac cttatggcgc
    11341 atttcattac ccagcgatgc gccgatgatg gatttacccg gcgagcaact gatcgactgg
    11401 ggcggagcgt tacgctggct gaaatcgaca gccgaggaca atcaaatcca tcgcatcgcc
    11461 cgcaacgctg gcggtcatgc gacccgcttt agtgccggag atggtggctt tgccccgcta
    11521 tcggctcctt tattccgcta tcaccagcag cttaaacagc agctcgaccc ttgcggcgtg
    11581 tttaaccccg gtcgcatgta cgcggaactt tgaggagcag gctatgcaaa cccaattaac
    11641 tgaagagatg cggcagaacg cgcgcgcgct ggaagccgac agcatcctgc gcgcctgtgt
    11701 tcactgcgga ttttgtaccg caacctgccc aacctatcag cttctgggcg atgaactgga
    11761 cgggccgcgc gggcgcatct atctgattaa acaggtgctg gaaggcaacg aagtcacgct
    11821 taaaacacag gagcatctcg atcgctgcct cacttgccgc aattgtgaaa ccacctgtcc
    11881 ttctggtgtg cgctatcaca atttgctgga tatcgggcgt gatattgtcg agcagaaagt
    11941 gaaacgccca ctgccggagc gaatgctgcg cgaaggattg cgccaggtag tgccgcgtcc
    12001 ggcggtcttc cgtgcgctga cgcaggtagg gctggtgctg cgaccgtttt taccggaaca
    12061 ggtcagagca aaactgcctg ctgaaacggt gaaagctaaa ccgcgtccgc cgctgcgcca
    12121 taagcgtcgg gttttaatgc tggaaggctg cgcccagcct acgctttcgc ccaacaccaa
    12181 cgcggcaact gcgcgagtgc tggatcgtct ggggatcagc gtcatgtcag ctaacgaagc
    12241 aggctgttgt ggcgcggtgg actatcatct taatgcgcag gagaaagggc tggaacgggc
    12301 gcgcaataat attgatgcct ggtggcccgc gattgaagca ggtgccgagg caattttgca
    12361 aaccgccagc ggttgcggcg cgtttgtcaa agagtacggg cagatgctga aaaacgatgc
    12421 gttatatgcc gataaagcgc gtcaagtcag tgaactggcg gtcgatttag tcgagcttct
    12481 gcgcgaggaa ccgctggaaa aactggcagt tcgcggcgat aaaaaactgg ccttccactg
    12541 cccgtgtacc ctacaacatg cgcaaaagct gaacggcgaa gtggaaaaag tgttgcttcg
    12601 tcttggattt accttaacgg acgttcccga cagccatctg tgctgcggtt cagcgggaac
    12661 atatgcgtta acgcatcccg atctggcacg ccagctgcgg gataacaaaa tgaatgcgct
    12721 ggaaagcggc aaaccggaaa tgatcgtcac cgccaacatt ggttgccaga cgcatctggc
    12781 gagcgccggt cgtacctctg tgcgtcactg gattgaaatt gtagaacaag cccttgaaaa
    12841 ggaataacaa aatgaaaact aaagtcattc ttagccagca aatggcgagt gcaattattg
    12901 ccgcaggtca ggaagaggcg cagaaaaata actggtcggt ttccattgct gttgccgatg
    12961 acggcggtca tctgctggcg ttaagtcgca tggacgattg cgcgccgatt gcggcttaca
    13021 tctcccagga gaaagcgcgt accgccgcgc tggggcgtcg tgaaactaag ggctatgaag
    13081 agatggtgaa caacggacgt accgcgttcg tgactgcgcc gttactgacg tcgctggaag
    13141 gcggcgtacc agttgttgtg gatgggcaaa ttattggtgc cgtgggcgtt tctggtttaa
    13201 ccggagcaca ggatgcgcag gtcgcgaaag cggcagcagc ggtgttggcg aaataagcga
    13261 aaacgaggag ataaacaatg agtcaaacca taacccaggg ccgtttacgc attgacgcca
    13321 attttaaacg ttttgtggat gaagaagttt taccgggaac agggctggac gctgcggcgt
    13381 tctggcgcaa ttttgatgaa atcgttcacg atttggcacc agaaaatcgt cagttgctgg
    13441 cagaacgcga tcgcattcag gcggcgcttg atgagtggca tcgcagcaat ccggggccgg
    13501 taaaagataa agcggcctat aaatctttcc tgcgtgaact gggctacctg gtgccgcaac
    13561 tggagcgcgt gacggtggaa accacgggca ttgacagcga aatcaccagt caggcggggc
    13621 cacagctggt ggttccggca atgaacgccc gctacgcgct gaacgcggcg aacgctcgct
    13681 ggggctcact gtacgatgcg ttatacggca gcgacatcat cccgcaggaa ggggcgatgg
    13741 tcagcggcta cgatccgcaa cgcggtgagc aggttatcgc ctgggttcgg cgtttcctcg
    13801 atgaatctct accgctggaa aacggcagct atcaggatgt ggtggcgttt aaggtggtcg
    13861 ataaacaatt acgcatccag ttgaaaaatg gtaaagaaac cacgttacgt accccggcgc
    13921 agtttgtcgg ttaccgtggc gatgccgctg cgctgacctg cattttgctg aaaaataacg
    13981 gcctgcatat tgaactgcaa attgatgcca acgggcggat tggcaaagac gatccggcgc
    14041 acatcaacga tgttatcgtc gaagcggcca tcagtaccat tctcgactgc gaagattcgg
    14101 tcgcggcggt tgatgcggaa gataaaatcc tgctgtaccg caatctgctg ggcctgatgc
    14161 aggggactct gcaagagaaa atggagaaaa acggtcgaca aatcgtgcgt aaactgaatg
    14221 acgatcgtca ttacaccgcc gccgatggct ctgaaatttc tctgcacgga cgctcgctgc
    14281 tgtttatccg caacgtgggt catttgatga ccattcctgt gatttgggac agcgaaggca
    14341 atgaaatccc ggaaggcatt cttgatggcg tcatgactgg cgcgatcgcc ctctatgatt
    14401 taaaagtgca gaaaaactcg cgcactggca gcgtctatat tgtgaaaccg aaaatgcacg
    14461 gtccgcagga agtggcgttc gccaacaaac tgtttacccg cattgagaca atgctcggta
    14521 tggcaccgaa taccctgaaa atgggcatta tggatgaaga acgtcggacc tcgctgaact
    14581 tgcgtagctg tatcgctcag gcgcgcaatc gcgtggcgtt catcaatacc ggtttcctcg
    14641 accgtaccgg cgatgaaatg cattcggtga tggaagctgg cccgatgctg cgtaaaaacc
    14701 agatgaaatc gacgccgtgg atcaaagcct acgagcgtaa taacgtgctt tccggtctgt
    14761 tctgtgggct gcgcggtaaa gcgcaaattg gtaaaggcat gtgggcaatg ccggacctga
    14821 tggcagacat gtacagccag aagggcgacc aactgcgtgc cggggcaaac acagcctggg
    14881 ttccgtcacc aaccgctgct acgctccatg cgctgcacta ccaccaaacc aacgtacaga
    14941 gcgtgcaagc caacattgcc cagaccgagt tcaatgctga atttgaaccg ctgctggacg
    15001 atctgctgac tattccggtg gctgaaaacg ctaactggtc ggtggaagag atccaacaag
    15061 agctggataa caacgtgcag gggattctgg ggtacgtggt gcgctgggtg gagcagggga
    15121 ttggttgttc aaaagtgccg gatattcaca atgtggcgct gatggaagac cgcgcaacgc
    15181 tgcgtatctc cagccagcat atcgccaact ggttacgtca cggtattctg accaaagaac
    15241 aggtgcaggc gtcgctggag aatatggcga aagtggttga tcagcaaaac gctggcgatc
    15301 cggcttatcg tccgatggcg gggaatttcg ctaactcgtg tgcttttaaa gctgccagcg
    15361 atttaatctt cctcggcgtg aaacagccaa acggctatac cgaaccgtta ttacacgcct
    15421 ggcgtttacg cgaaaaagaa agtcattaat agcgtcaaaa aaagccccgg caagttttat
    15481 tgtcggggct atgatagtaa ctgcggtagt acatttatcc cggaaataac cggagcgatt
    15541 atcagggttt aacaatatta aatacccgta gcgcattact agcgggccta aaacaacaat
    15601 atggacatct taacgatact ctgcgcctgc gtgtgcggaa cagaaaccct tacccgatat
    15661 acttgttatg ccagaggtag caattgcgtt agcggcgtac tgttgctgta aagtataacg
    15721 cctatatttt acagttcagt cctcttttgc ttattacaac cttaagtata tcaagcatat
    15781 aaagataata agagactgaa caatatggtt acctggaccc aaatgtatat gccgatggga
    15841 ggactggggc tatccgctct ggtcgccctg atcccgataa tattcttctt cgttgcactc
    15901 gcggtattac gtctgaaagg acatgtcgct ggagcaataa cccttatctt atctatcctg
    15961 attgcgatat tcgcctttaa aatgccgatt gatatggcat ttgctgctgc aggctatggc
    16021 tttatttatg gattatggcc aattgcgtgg attattgtcg cggcggtgtt cctctataaa
    16081 ttaaccgttg ccagcggtca gttcgatatt atccgcagct cggttatctc catcaccgac
    16141 gatcagcgtt tgcaggtgtt actgattggt ttctcctttg gtgcgttgct ggaaggagcg
    16201 gctggctttg gtgcgccggt ggcgattacc ggtgcgctgc tggtgggcct gggcttcaaa
    16261 ccgttatacg cggcggggct gtgtctgatt gccaacactg cgccagtggc gtttggtgcg
    16321 ttgggcgtgc cgattctggt cgccggtcag gtaacgggaa tcgatccgtt ccacattggc
    16381 gcaatggcgg gacgtcagtt accgttcctg tcggttcttg tgccgttctg gctggtagca
    16441 atgatggacg gctggaaagg ggtgaaagag acgtggccag cggcgctggt tgctggggga
    16501 agcttcgctg tcactcagtt ctttacctct aactatattg gtccggaact gccggatatt
    16561 acttcggcgc tggtgagtat cgtctcactc gctttattcc ttaaagtctg gcggccgaaa
    16621 aataccgaaa cggcaatcag catggggcaa tccgcaggtg cgatggtggt aaataagcca
    16681 tcttctggcg gtcccgtgcc ttcagaatat agtctggggc aaatcattcg tgcgtggtca
    16741 ccgtttttaa tcttaacggt gctggtcacc atctggacca tgaagccgtt taaagcgtta
    16801 tttgctccgg gcggcgcgtt ttattcactg gtgattaatt tccagatccc tcatttgcat
    16861 caacaagtgt tgaaagcggc acccattgtc gcccaaccaa cgccaatgga tgcggtgttt
    16921 aaattcgacc ctctctcggc tggcggcacc gctattttta ttgcggcgat tatctctatc
    16981 ttcatcctcg gcgtgggcat caagaaaggg attggcgtct ttgccgaaac gctaattagt
    17041 ctgaagtggc cgatattgtc gattggcatg gtgctggcgt tcgccttcgt caccaactat
    17101 tccggcatgt ccaccacgtt ggcgctggta ctggcaggta caggcgtgat gttcccgttt
    17161 ttctcaccgt tcctcggctg gctgggggta ttccttaccg gctcggacac ttcttcaaac
    17221 gccctgtttg gttcactgca atcgaccacg gcgcagcaaa tcaacgtctc tgacactctg
    17281 ctggtggcag caaataccag cggcggcgta actggcaaga tgatctcccc gcaatctatc
    17341 gccgtggcct gcgccgcgac gggcatggtg ggccgagaat ctgaactatt ccgctacacc
    17401 gtgaagcaca gtctgatttt tgccagcgtt atcggcgtta tcacgttgtt gcaggcgtat
    17461 gtgtttactg gaatgttggt ctcgtaaatc aataatgccg gatgatcgtt aatatcatcc
    17521 ggcttcattt acattattct gtcttattaa ttgaaaatta tttctcgctt tattatccat
    17581 gcggcaataa tgttttgtgt tcagataacc tgctaaataa ttaggagcgt gagaacatct
    17641 atttttttat cacgcaaaac aagtctgtat taattatgtt cgcttgatga gcaaacaccg
    17701 ttctatctgt tgcttttgca atcttaatcg ttaaaactac caacgaattt atatagttgc
    17761 gttgtgaaat gttgttaatt ttttgtttat tgaaatttcg taaaaatagc cagccagaca
    17821 tcaaataggg gttattctta taaatttcac ctgttttcta tcaatgatgt cgttttctta
    17881 agaatggagg aaattattaa agaagataaa gcataaaact atttctccca gttacgaatt
    17941 ttttaacatt gttttgtcac ttgcgttatt aatgaataag aaatttaaat ataagaaatc
    18001 gcttttagcg gctattttga gtgcaaccct gttagccggt tgtgatggcg gtggctccgg
    18061 atcttcctcc gatacgccgc ctgtagattc tggaacaggg tctttgccgg aagtgaaacc
    18121 tgatccaaca ccaaacccgg agccgacgcc tgagccaacg ccggacccag aacctacgcc
    18181 ggaaccgaca cctgatcctg agccaacacc agaaccggag ccagaacctg ttcctacgaa
    18241 aacgggttat ctgactctgg gcggaagcct gcgggtaact ggtgatatca cctgtaatga
    18301 tgaatccagc gatggcttta cctttacacc aggcgacaaa gtcacctgtg tggcagggaa
    18361 caacacgaca attgctacct tcgacaccca gtcagaagct gcgcgtagcc tgcgtgcggt
    18421 tgaaaaagtg tcgtttagtc ttgaggacgc gcaagaactg gcgggttccg acaacaagaa
    18481 aagcaatgcg ctctcgctgg tcacctccat gaacagttgc ccggcgaata cagaacaggt
    18541 gtgcctggag ttctcctcgg tgatcgagag taaacgtttc gactcgctgt ataagcaaat
    18601 cgatctggca ccggaagagt tcaaaaagct ggtcaatgaa gaggtggaaa acaatgccgc
    18661 gactgataaa gcgccatcca ctcatacttc accggtagtc cccgtcacga ctccgggaac
    18721 aaaaccggat ctgaacgcct ccttcgtgtc ggctaacgcg gaacagtttt atcagtatca
    18781 accttcggaa atcattctct cagaaggtcg cctggtggat agccagggat atggtgttgc
    18841 tggcgtcaac tactacacca attcaggccg tggcgtgaca ggggaaaatg gtgaattttc
    18901 ttttagctgg ggcgaaacca tctcctttgg tatcgatacc tttgaactgg gttcagtgcg
    18961 cggcaataag tcgaccattg cgctgactga actgggtgat gaagttcgcg gggcgaatat
    19021 cgatcagctt attcatcgct attcgacgac cgggcaaaat aatacccgtg ttgttccgga
    19081 tgatgtacgc aaggtctttg ccgaatatcc caacgtgatc aacgagatta tcaatctctc
    19141 gttatccaac ggtgcgacgc tggatgaagg tgagcaagtt gttaatctgc caaacgcatt
    19201 tatcgagcag tttaagacgg gtcaggccaa agagatcgat accgcgattt gtgcgaaaac
    19261 cgacggttgt aacgaggctc gctggttctc gctgacgacg cgcaatgtta atgacggcca
    19321 gattcagggc gttatcaaca agctgtgggg cgtggatacg aactacaaat ctgtcagcaa
    19381 gttccatgta ttccatgact ctaccaactt ctatggcagc acgggtaatg cgcgcggtca
    19441 ggcggtggtg aatatctcca acgcggcctt cccgattctg atggcgcgta atgataaaaa
    19501 ctactggctg gcgtttggcg aaaaacgcgc ctgggataaa aatgagctgg cgtacattac
    19561 ggaagcgcct tctcttgttg agccggaaaa cgttacgcgc gataccgcca ctttcaacct
    19621 gccgtttatt tcgctggggc aagtcggtga gggcaaactg atggttatcg gtaacccaca
    19681 ctacaacagc attttgcgtt gcccgaacgg ttacagctgg aacgggggcg ttaataaaga
    19741 tgggcagtgt acgctcaaca gcgacccgga tgacatgaag aacttcatgg agaacgtgct
    19801 gcgctatctg tccgacgata aatggacgcc ggacgcgaaa gccagcatga ccgtaggcac
    19861 caacctggat actgtctatt ttaaacgtca tggtcaggtt acaggaaaca gcgctgcgtt
    19921 cgacttccat ccggattttg cgggcatctc tgttgagcat ttaagtagct atggcgatct
    19981 cgatccgcag gaaatgccgc tgctgatcct taacggcttt gaatatgtga ctcaggtggg
    20041 taacgatcct tatgcaatcc cgctgcgtgc agataccagc aaaccgaagc tgactcagca
    20101 ggatgtgacc gatctgatcg cctatctgaa caaaggtgga tcggtgctga tcatggaaaa
    20161 cgtgatgagc aatcttaagg aagagagcgc gtctggtttt gtgcgtctgt tggatgccgc
    20221 aggtctgtcg atggcactga acaagtcggt agtaaataac gatccgcaag ggtatccgaa
    20281 ccgcgttcgt cagcagcgcg caacgggcat ttgggtctat gaacgttatc ctgccgtaga
    20341 tggtgcgctg ccgtacacca tcgatagtaa gacaggggaa gttaagtgga aatatcaggt
    20401 agaaaacaaa cctgatgaca aaccgaagct ggaagttgcc agctggctgg aagatgtaga
    20461 tggcaaacag gaaacgcgtt atgcctttat tgatgaggcc gatcataaaa cagaggattc
    20521 tctgaaggct gcgaaggaga aaattttcgc cgcgttcccg gggctgaaag agtgtactaa
    20581 tccggcatat cactatgagg tcaactgtct ggaatatcgt cctggcacgg gggttccggt
    20641 tactggtggc atgtatgttc cacagtatac gcaactaagc cttaacgccg acactgcaaa
    20701 agcgatggtg caggctgcgg atttaggcac caacattcag cgtctgtatc agcatgagct
    20761 ctacttccgg accaatggtc gcaaaggtga gcgtctgagc agcgtcgatc tggaacgtct
    20821 gtaccagaac atgtcggtct ggctgtggaa cgatacgagc tatcgttatg aagaaggcaa
    20881 aaatgacgag ctgggcttta aaacgttcac cgagttcctg aactgctacg ccaatgatgc
    20941 ctatgcaggc ggcaccaagt gttctgcaga tctgaaaaaa tcgctggtcg ataacaacat
    21001 gatctacggt gacggtagca gcaaagcggg catgatgaac ccgagctacc cgctcaacta
    21061 tatggaaaaa ccgctgacac gcctgatgct gggccgttcc tggtgggatc tgaacatcaa
    21121 agttgatgtc gagaagtatc cgggagcagt atcggaagag ggacagaacg ttactgaaac
    21181 catcagcctg tactcgaatc cgaccaaatg gtttgcaggt aacatgcagt caactggcct
    21241 gtgggcaccg gctcagaaag aggtcaccat taagtccaat gcgaacgttc ctgtgaccgt
    21301 caccgtggcg ctggctgacg acctgaccgg acgtgagaag catgaagttg cgctgaaccg
    21361 tccgccaaga gtgactaaaa cgtactctct ggacgctagc ggtacggtga agttcaaggt
    21421 gccttacggt ggcctgattt atatcaaggg caatagctct accaatgaat ctgccagctt
    21481 cacctttact ggcgtggtaa aagcaccgtt ctataaagac ggcgcatgga aaaacgatct
    21541 gaactcaccg gctccgctgg gtgagctgga atcagacgct ttcgtctata ccacaccgaa
    21601 gaagaacctg aatgccagca attacactgg cggactggag caattcgcta acgatctgga
    21661 cacctttgcc agatcgatga atgacttcta cggtcgtgat gaaacgagcg gtaagcaccg
    21721 gatgtttacc tataaagcat tgacgggaca taaacatcgt ttcacaaacg atgtgcagat
    21781 ctccatcggt gatgcgcact cgggttatcc ggtaatgaac agcagcttct cgacgaacag
    21841 caccacgctg ccgacgacgc cgctgaacga ctggttgatc tggcacgaag tagggcacaa
    21901 cgccgcagaa acaccgctga ctgtaccggg tgcaactgaa gtcgcgaaca acgtgctggc
    21961 gctgtacatg caggatcgct atctcggcaa gatgaaccgt gtcgctgacg atattaccgt
    22021 cgcaccggaa tatctggagg agagcaacaa ccaggcatgg gcacgcggcg gtgcgggtga
    22081 ccgtctgctg atgtacgcgc agctgaagga atgggcagag aaaaactttg atatcaagaa
    22141 atggtatcca gatggcactc ctctgccaga gttttacagc gagcgtgaag ggatgaaagg
    22201 ctggaacctg ttccagttga tgcaccgtaa agcgcgcggc gatgacgttg gtgacaagac
    22261 ctttggtggc aagaattact gtgctgaatc caacggtaac gctgctgaca cgctgatgct
    22321 gtgtgcatcc tgggtcgctc agacggatct ttcggagttc tttaagaaat ggaatccggg
    22381 tgcgaatgct taccagctgc cgggggcgag cgagatgagc ttcgagggcg gtgtgagcca
    22441 gtcggcgtac aacacgctgg cgtcactcaa tctgccgaaa ccgaagcaag ggccggaaac
    22501 cattaataag gttaccgagt attcgatgcc tgctgaataa cccggataag gcgttcacgc
    22561 cgcatccggc atgaaataag gcgcaccggg ttgcgccagc ttattgggcc tggaggaaag
    22621 ccatatggca tacacctgca ggcccgcatc cggcaagtta caacaaacaa cctttaacca
    22681 tgctttttga tgtttttcag caataccccg cggcgatgcc catactggct accgtcggag
    22741 gattgattat cggcagtttt ttgaatgtgg tgatttggcg ttaccccatc atgctgcgcc
    22801 aacaaatggc ggagtttcat ggtgaaatgc cgagtgcgca gtcaaaaata agccttgcgc
    22861 taccgcgttc gcactgccca cattgtcagc agaccatccg gatacgtgac aatattccgc
    22921 tgctctcctg gctgatgctc aaagggcgct gccgcgactg tcaggcgaaa atcagcaagc
    22981 gttatccgct ggtggagtta ttgacagcac tcgctttttt gctggcgagt ctggtctggc
    23041 cggaaagtgg atgggcgctg gcggtgatga tattatccgc ctggctgatt gccgcgagcg
    23101 tcattgacct cgatcaccaa tggctgcccg atgtttttac tcagggcgta ttgtggacgg
    23161 gactaattgc ggcatgggcg cagcagagcc cgctcacgct acaagatgca gtcaccggcg
    23221 tcctggtggg gtttatcact ttttactctc tgcgctggat agccggaata gttctgcgta
    23281 aagaagcatt aggcatgggc gatgtattat tgttcgctgc gttaggtagt tgggtggggc
    23341 cgttgtcact gcccaatgtt gctttaatcg cctcatgctg cggcctgata tatgccgtta
    23401 ttataaaaaa aggatcaacc acactgcctt ttggaccgtg tttaagtctg ggcggtatag
    23461 caacacttta tctacaggca ttgttttaat gataaccacg tcattatcaa agtgacattt
    23521 taactcttat taataacctt agagattatt taccatgtcg ataaaacaga tgccagggag
    23581 ggtattaata tcgctattgt tgagcgttac aggattatta agtggctgtg ccagccataa
    23641 tgaaaacgcc agtttgctgg cgaaaaaaca ggcgcaaaat atcagccaaa acctgccgat
    23701 taaatctgcg ggatatacct tagtgctggc acaaagtagc ggcacgacgg taaaaatgac
    23761 cattatcagc gaagcgggta ctcagaccac gcagacgcca gacgcctttt taatcagcta
    23821 tcaacgacaa atgtgcgctg acccaacagt gaaattaatg ctcactgagg gaattaatta
    23881 cagcataacg attaatgata cacgtacagg taaccagtat cagcggaaac tggatcgtac
    23941 cacctgtgga atagtcaaag cataacgtcg ggtagatata aattggcgcg ggttgttttt
    24001 cgtgacgcac gaatttatct cattcaatgg ctgacaaaaa ttcgtcacac tcttaaccag
    24061 agacaatctc ttaatacaga caaagagcat ctgcgaaaaa ttgtacgcgg gatgttctgg
    24121 ctgatgctgc ttattatttc tgcaaaagtg gcgcattcac tctggcgcta tttctccttt
    24181 tctgcggaat atacggctgt ttccccatcg gcgaataaac cgccccgtgc ggatgcaaaa
    24241 acgttcgata aaaatgacgt gcaattaatc agccagcaaa actggtttgg caaatatcag
    24301 cccgtcgcca cgccggtaaa acaacccgaa cctgtgcctg tggcagaaac gcgtcttaat
    24361 gtggtgctgc gcgggattgc ctttggtgcc agacccggcg cggttattga agaaggcggt
    24421 aaacagcagg tctatttgca gggtgaaacg cttggctcgc acaatgcggt gattgaggaa
    24481 atcaatcgcg accatgtgat gctgcgctat cagggaaaaa tagagcgcct gagcctggct
    24541 gaagaggagc gttccactgt tgccgtgacc aacaaaaaag ctgtcagtga cgaagcaaag
    24601 caagctgttg ctgagcctgc tgccagtgcg ccagttgaga tcccgactgc cgtgcgtcag
    24661 gcactggcga aagatccgca gaaaattttt aactatatcc agcttacgcc tgtgcgtaag
    24721 gaggggattg tcggttatgc agtgaagccg ggggcagatc gttctctgtt cgatgccagc
    24781 ggtttcaagg aaggcgatat cgccattgcg ctaaatcagc aggatttcac tgatccacga
    24841 gcaatgattg ctctgatgcg gcagttacct tcaatggatt ccattcaact tacggtttta
    24901 cgcaagggtg cgcgccacga catttccatc gcgctgcgct aaccgcattt aatccaggag
    24961 aatcattcat cgtgttttgg cgtgatatta cgttgtcggt ctggcgtaag aagacaactg
    25021 gcctcaaaac aaaaaagcgt ttactgccgc tggtgctggc agcggcatta tgcagttcac
    25081 cggtctgggc ggaagaagcc actttcaccg ctaattttaa agataccgac ctgaaatcgt
    25141 tcatcgaaac cgtcggcgct aaccttaata aaaccatcat tatggggccg ggcgtacagg
    25201 ggaaagtgag tattcgcacc atgaccccgc tcaatgaacg ccagtattac cagctattcc
    25261 ttaacctgct ggaagcacag gggtatgccg tcgtaccgat ggaaaacgac gtgctgaagg
    25321 tggtgaaatc cagcgctgcg aaagtcgagc cgctgccgct ggtcggtgaa ggcagcgaca
    25381 actacgcggg cgatgaaatg gtcaccaaag tagtgccggt acgtaatgtg tcggtacgcg
    25441 agctggcacc gattctgcgc cagatgatcg acagcgcagg ctcaggcaat gttgttaatt
    25501 acgatccctc caacgtgatt atgctcaccg gacgcgcctc cgttgtggag cgcctgacag
    25561 aagtgatcca gcgcgtggat cacgcgggta atcgcaccga agaggtgatc ccgctggata
    25621 acgcctcggc ttcggaaatc gcccgcgtgc tggaaagcct gacgaaaaac agcggcgaga
    25681 accagcctgc gacgctgaaa tctcaaattg tcgccgacga acgcaccaac agtgtgattg
    25741 tcagtggtga cccggccacg cgggacaaaa tgcgccgcct gatccgtcgg ctggactcag
    25801 aaatggaacg cagcggcaac agccaggtgt tctatctcaa atacagcaaa gccgaagatc
    25861 tggtcgatgt gctgaagcag gtcagcggca cgctcacggc ggctaaagaa gaggcggaag
    25921 gcacggtcgg tagcgggcgt gagattgtat ccatcgccgc cagcaaacac agtaatgccc
    25981 tgattgtaac cgcaccgcag gacatcatgc agtcgctgca aagcgtgatt gaacaactgg
    26041 atattcgccg tgctcaggtg cacgtcgagg cgttgatcgt ggaagttgcc gaaggtagca
    26101 atatcaactt cggcgtgcag tgggcgtcga aagatgccgg attaatgcag tttgctaatg
    26161 gtacgcagat ccctatcggt acgctggggg cagccatttc tcaggcgaaa ccgcagaaag
    26221 gctcgacggt gatcagtgaa aacggcgcta ccaccattaa tccggatacc aacggcgatc
    26281 tctccacact cgcccagctt ctttctggat ttagcggtac ggcggttggc gtggtgaaag
    26341 gcgactggat ggcactggtg caggcggtta aaaacgactc cagctcgaac gttctctcca
    26401 cgccgagcat cactacgctg gacaaccagg aagccttctt catggtgggc caggacgttc
    26461 cggtattaac cggctctacc gttggctcca ataacagcaa tcctttcaat acagtagaaa
    26521 ggaaaaaagt cggcatcatg ctgaaagtca cgccgcagat taacgaaggt aacgcggtac
    26581 agatggtgat tgagcaggaa gtctcgaagg tggaaggaca gaccagcctc gacgtggtgt
    26641 ttggtgagcg caaactgaaa accaccgtgc tggctaacga tggtgagctg attgtgcttg
    26701 gtggtctgat ggacgatcag gcgggagaaa gcgtggcgaa agtgccgctg ctgggcgata
    26761 tcccattgat tggtaacctg tttaaatcga cggcggataa aaaagaaaaa cgtaacctga
    26821 tggtgtttat ccgtccgacc attctgcgtg acggtatggc ggcagacggc gtgtcgcagc
    26881 gcaaatataa ctacatgcgc gccgagcaga tctatcgcga tgagcaaggt ttaagcctga
    26941 tgccgcacac cgcgcagccg gtactgccag cgcaaaatca ggctctaccg ccggaagttc
    27001 gtgcattcct taatgccggg agaacgcgtt aatggtgcct gtagcacagg aaaccaccgc
    27061 taacaccgtg cgtctgccct acagtttcag ccgtcggttt agcctggtgg catggtgcga
    27121 agcgtcgctg gagatcctcc atgtgcatcc gttgtcgctc tctgttttgc aggagctgca
    27181 gcgggggctg aacgcgccct ttacgctgcg gcaaatcgac ggggccgaat ttgaacagcg
    27241 gctgaatgcg gtctggcagc gggactcttc cgaggctcgc cagctgatgg aagatctcgg
    27301 ttctgctgag gactttttta ctctcgcgga agaactgcca gaaacggaag atctgctgga
    27361 aagtgacgac gatgcgccga tcatcaaact gatcaacgcc atgctggcag aggcgattaa
    27421 agaaggcgct tcggatatcc acatcgagac gtttgaaaag agtctggtga tccgttttcg
    27481 cgttgacggc acattacatg aaatgctgcg cccggggcgc aaactggcct cgctgctggt
    27541 gtcgcgtatc aaggtgatgg cgcggctgga cattgccgaa aagcgcgtgc cgcaggatgg
    27601 acgtattgcg ctgttgctgg gcggccgggc gattgacgtg cgtgtctcca ccatgccttc
    27661 tgcctggggc gagcgcgtgg tgctgcgact gctggacaaa aaccaggccc gcctgacgct
    27721 ggagcgtctg gggcttagcc agcaactgac cgcgcagttg cgccagctgt tacacaaacc
    27781 gcacggcatc tttctggtga cggggccgac gggttccggc aaaagcacca cgctgtacgc
    27841 cggattgcag gagctgaaca accattcgcg caacattctc acggttgaag atcccatcga
    27901 atacatgatt gaagggatcg gtcagacgca ggttaacacc cgcgtcggca tgacctttgc
    27961 ccgtgggctg cgcgcgattt tgcgtcagga cccggatgtg gtgatggtcg gtgaaattcg
    28021 cgataccgaa accgcagaaa tcgccgtcca ggcttcactt accggacacc tggtcctttc
    28081 cacgctgcat accaacacag cggtgggggc gatcacacgt ttgcaggata tgggcgtgga
    28141 gcctttcctg ctctcttcca gtctgacggg cgtgatggcg cagcgactgg ttcgcacgct
    28201 gtgtcccgac tgccgccagc ccgcaccagc cactgacgaa gaaaaacgcc tgctgggaat
    28261 taccgacgcc cgtaccgtca ctctgtacca tccacagggc tgtcccgcct gtaatcacaa
    28321 aggttttcgc ggacggactg ccatccatga gctgatcgtg gtggatgcca cattgcgtga
    28381 tttgatccac cgtcaggccg gggagctgga gctggaacgt tatgtccgac aacactctgc
    28441 gggtatccgc agcaacggca ttgagaaagt gctcgccgga gaaacctctc tcgatgaagt
    28501 tctgcgggta accatggagg cgtaatggca ctgttttact atcaggcgct ggagcgtaat
    28561 ggtcgcaaaa ccaaaggtat gattgaggcg gattccgcgc gtcatgcccg ccagttgttg
    28621 cgcggtaaag agcttatccc cgtgcatatt gaagcccgga tgaatacttc gtcagggggg
    28681 atgttgcagc gtcggcggca cgcacatcgt cgcgtggcgg cggcagatct tgcgctgttc
    28741 acgcgccaac tggcaacgct ggtacaggca gcaatgccgc tggaaacctg cttacaggcg
    28801 gtcagtgagc aaagtgaaaa actgcatgta aaaagcctcg gaatggcgct gcgcagccgg
    28861 attcaggaag gttacaccct gtcggacagc ctgcgcgaac atccccgcgt ctttgattct
    28921 ctgttttgtt cgatggttgc tgccggagaa aaatccgggc atctcgacgt ggtgcttaat
    28981 cgcctggcag attacaccga acagcgacag cgcctgaaat cacgtctatt gcaggccatg
    29041 ctctatccgc tggttctgct ggtggtggca acgggcgtag tcactatttt gctgacggca
    29101 gtggtgccga aaatcatcga acagtttgat catctcggac acgcgctacc cgcctcaacc
    29161 cgcacgctta ttgccatgag cgacgcgtta caggccagcg gcgtttactg gctggcggga
    29221 ttgctggcgc ttctggtgct ggggcaacgg ctacttaaaa atcctactat gcgcctgcgc
    29281 tgggataaaa ccgtgctgcg tctgcccgtg acagggcgtg ttgcgcgcgg gctgaatacg
    29341 gcgcgttttt cccgcacctt aagcatcctc accgccagca gtgttccgct actggaaggt
    29401 attcagaccg ctgccgctgt gtcggcaaat cgctatgtcg aacaacaact actgctggcg
    29461 gcagatcgcg tccgcgaagg aagcagtctg cgcgccgcgc tggcggagtt gcgcctgttc
    29521 cccccgatga tgctgtacat gatcgcctcc ggcgaacaga gcggcgaact ggaaaccatg
    29581 cttgagcagg ccgctgttaa ccaggaacgg gaatttgata cccaggtggg gctggcgttg
    29641 gggctgtttg agcctgcgct ggtggtgatg atggcgggcg tggtgctgtt tattgtcatc
    29701 gccatcctcg agccgatgct gcaactgaat aatatggttg gaatgtaatt tacggagtta
    29761 tcacatgaat tcgttatccc gcacacaaaa accacgggca ggttttaccc tgctggaagt
    29821 gatggtggtg attgttattc ttggcgtcct ggcaagtctg gtggtgccta acctgttggg
    29881 caacaaagag aaagccgatc ggcaaaaagc catcagcgat atcgtggcgc tggaaaacgc
    29941 actggatatg taccgactgg ataacgggcg ttatccgacc acagaacagg ggcttgaggc
    30001 gctgatccag cagccagcca atatggcgga ctcccgcaac taccgtactg gtggatacat
    30061 taaacgactg ccaaaggatc cgtggggcaa tgattatcag tatctcagtc cgggtgaaaa
    30121 agggctgttt gatgtttata ccctgggggc ggatggtcag gaaaacgggg aaggcgctgg
    30181 cgcagatatc ggtaactgga atttgcagga gtttcagtaa gcagtgcctg aacgcggatt
    30241 cacacttctg gaaatcatgc tggtgatttt tcttatcggc cttgccagtg cgggcgtggt
    30301 acagactttt gcgaccgatt ctgaatcgcc cgcgaaaaaa gcggcgcagg attttctgac
    30361 tcgctttgcg cagtttaagg acagggcagt gatcgaaggg aaaacactcg gtgtgctaat
    30421 cgacccgcct ggctaccagt ttatgcagcg tcgtcagggg cagtggtcgc ccgtttcctc
    30481 gacccggtta tcggcacagg ttacggtgcc gaaacaggtg cagatgctgt tacaacccgg
    30541 aagtgatatc tggcagaagg aatatgcgct ggagctgcaa cgtcgtcgac tgacgctgca
    30601 cgatattgaa ctggagctgc aaaaagaggc gaaaaagaag actccgcaga tccgtttttc
    30661 gccttttgaa cccgccgcgc cgtttacgct gcgcttctac tcggcggcgc aaaacgcatg
    30721 ttgggcggta aaactggcgc acgatggcgc gttatccctc aatcaatgtg atgagaggat
    30781 gccatgaagc gcggatttac cttgctggaa gtgatgctcg cgctggcgat ttttgcgctg
    30841 gctgccacgg cggtgttaca gattgccagc ggtgcgctga gtaatcagca aattcttgag
    30901 gaaaaaacgg tagcgggctg ggtggctgaa aaccagaccg cgctgctcta cctgatgacc
    30961 cgcgagcagc gggcggtcag gcagcagggc gagagcgata tggcaggaag ccgctggtac
    31021 tggcgaacca caccgctgag tacaggcaac gcgctgctcc aggcggtgga tattgaagtc
    31081 agccttcacg aagacttttc gtcggtgatt cagtcacgac gcgcctggtt tagcgccgtg
    31141 ggaggccaac agtgagaagg actcgcgctg gtttcacgtt actggaaatg ttggtggcaa
    31201 ttgccatttt tgcctcgctg gcactgatgg cgcagcaggt gacaaacggt gtcacacgcg
    31261 tgaataacgc cgtcgccgga catgatcaaa aactaaacct catgcagcaa acgatgagtt
    31321 ttctgaccca cgatctgacc caaatgatgc cgcgtccggt aagaggcgaa cagggccagc
    31381 gtgaaccagc gttactggcg ggcgctggcg tgctggcgtc tgagagtgaa ggcatgcgct
    31441 ttgtgcgcgg cggcgtggtt aacccgttga tgcgtctgcc acgcagcaat ctgctcaccg
    31501 tcggttaccg cattcatgac ggttatctcg aacggttagc ctggccgctg accgatgccg
    31561 caggcagcgt gaagccgaca atgcaaaagt tgattccggc ggattcgctc cgtttgcagt
    31621 tctatgatgg tacgcgctgg caggagagct ggtcgtcagt acaggcgatc cctgtggcag
    31681 tgcgcatgac tctgcattcg ccgcaatggg gcgagattga acgcatctgg ttgttacgcg
    31741 ggccgcaatt atcatgatca cctcaccacc aaaacgcgga atggcactgg tcgtggtgct
    31801 ggtattgctg gcggttatga tgctggtaac catcacgctt tccgggcgga tgcagcaaca
    31861 acttgggcga acgcgcagcc agcaggagta ccagcaggcg ctgtggtaca gcgccagtgc
    31921 ggaaagcctg gcgctgagcg cgctcagtct gagcctgaaa aatgaaaagc gcgtgcatct
    31981 gacacagccg tgggcatccg gccctcgttt cttccctctg ccgcaggggc aaattgcggt
    32041 caccctgcgt gacgcacagg cttgctttaa cctgaatgcc cttgctcagc cgacaacggc
    32101 gtcgcgtccg cttgcggtac aacagttgat tgccctgatc tcgcgcctgg atgtgcctgc
    32161 ttatcgggcc gaactgatag ccgaaagcct gtgggagttt attgacgagg accgcagcgt
    32221 ccagacacgt ctgggccgtg aagacagcga gtatctcgcc cgttcggtgc ctttctacgc
    32281 cgcgaatcaa ccgctggccg atatcagcga gatgcgcgtg gtgcagggaa tggacgacgg
    32341 gctttatcaa aaactgaaac cgctggtctg tgcgctgccg atgacccgcc agcaaatcaa
    32401 catcaatacc ttagacgtca cgcaaagtgt gcttcttgag gcgctgtttg acccgtggtt
    32461 aagccctgtt caggcgcggg cattattaca acaacgtccg gcgaagggct gggaagatgt
    32521 cgatcagttt cttgcacagc cgctacttgc tgacgtcgat gagcgtacta aaaaacagct
    32581 aaaaaccgtc ctgagcgtgg acagcaatta cttctggctg cgttcagata tcaccgtgaa
    32641 tgagattgaa ctgacgatga attcgttaat tgtccgcatg ggcccacaac acttttcggt
    32701 tctctggcat cagacaggag aaagtgagtg agttccatcc ttgagatttt tttcccgctt
    32761 tgcgcctccg ataccgtccg ctggcagcgc cgtacacccg acgtggaaca cggtatctgg
    32821 cctgacgttg ctgacgaaca gcttcagcaa tggctgcaaa ccgatgcgat tcgactctac
    32881 attcccggcg aatggatcag cgtctggcag gttgaactgc ctgatgtcgc ccgcaagcag
    32941 ataccgacta ttctgcccgc cttgctggaa gaagagctga accaggatat cgatgaactg
    33001 catttcgccc cgttgaaaat cgaccagcaa ctggcaaccg tagcagtgat tcaccaacag
    33061 catatgcgca acattgcgca gtggttgcag gaaaacggca tcacccgcgc taccgtcgcg
    33121 ccggactgga tgtccattcc ttgtgggttt atggctggcg atgcgcaacg ggttatctgc
    33181 cgcatcgatg aatgccgggg atggagcgcc gggctggcgc tggctccggt catgttccgc
    33241 gcccagctca atgagcagga tttaccgctt tcgctaaccg tagtcggcat tgcaccggaa
    33301 aagctatcag catgggctgg cgcagacgct gaacgcctga ccgttacagc tctgcccgcc
    33361 gttaccactt atggcgaacc ggaagggaac ctgctgacag ggccgtggca gcctcgcgtc
    33421 agctaccgaa aacagtgggc gcgctggcgg gtgatgattc tgccgatatt gctgattctg
    33481 gttgcgctgg cagtggagcg gggcgtgacg ttatggagcg tcagcgaaca ggtggcgcaa
    33541 agccgcaccc aggcggagga acagttctta acgttgttcc cggagcagaa gcggattgtg
    33601 aatttacgct ctcaggtgac gatggcgctg aaaaaatatc gcccacaggc cgacgatacc
    33661 cggctgctcg ccgagttgtc agcgatagcc agcaccctga aatcagcgtc actttccgac
    33721 atcgaaatgc gtggtttcac ctttgatcaa aaacgccaga cgcttcacct ccagttgcgg
    33781 gctgctaact ttgccagttt cgacaaactg cgtagtgcac tggcaaccga ttatgttgtg
    33841 caacaggacg cattacagaa agagggtgat gcggtttccg gcggcgtaac gttgcggagg
    33901 aaataacatg ttacgcgata aatttattca ctattttcag caatggcgtg aacgccagtt
    33961 aagccgtggc gaacactggc tggcacaaca cctggcgggg cgttctccgc gtgaaaaggg
    34021 catgttactg gcagcggtgg tgttcctgtt tagcgtcgga tattacgtcc tcatctggca
    34081 gccgttgagc gaacggatag aacaacagga gacaatgttg cagcaactgg tggcgatgaa
    34141 caccagactg aggagcgccg cgccggatat tatggcggcg cgaaaatccg ccacaacaac
    34201 gcctgcgcag gtatcgcggg ttatcagtga cagcgcttcc gcgcactcgg tagtcatcaa
    34261 gcggatagcc gagcgtgggg agaatattca ggtctggata gatccggtgg tgtttaatga
    34321 cctgctgaac tggttaaacg cgctggatga aaagtatgcg ctgcgggtga cacaaattga
    34381 tgccagtgct gctgagaaga ctgggatggt gaatgtgcag cggctggagt ttggacgggg
    34441 gtaaagtatc aggagattag tatcaataaa attactctcg ttaacgtggc gagagtaatt
    34501 cccccagact ttataagcaa acttttttcg tgaaggttat tgtcagatcc aaaagcaaaa
    34561 acccgccttg tgggcgggtt cttaagaatt agtggtgccg gactcggaat cgaagattgc
    34621 ttctatcatg ttgatattag tttattttgt ttggtttggt gaaagatgta ccctcttttg
    34681 tacccgcagt cccttttgat atcgaaaggg aatcaaactc ggggtcaggc tgagagcaca
    34741 gaaggaaagt gcctggctat tatatcgtta attctgtcaa tcagctccag ccttttgaaa
    34801 gtgatgtgtg cagatccctt cttaaagtat ttaatgatga acatctcatc ttcatagcgt
    34861 tctttaccct gaacggaccg tatgtggtca tccagattaa tggtgatatt cccccggttg
    34921 ttggggatcg gtttaccgct gagcagatgc aacattcttt ccaggtcagc caggcgatct
    34981 gcctgcattc cactgataag atgaaatccc catctgtccc atctcaccag gttgttgacg
    35041 ataattttac tgccaaattt acagggggaa ttggttttgt aattccagtt cagccctctg
    35101 aagacgttga tcaccccgcg ctcaaacacc tcatccttac tccggtgcag ttgttcgaat
    35161 gtactcagga tattcgcctt gctgatttcc ggaaagttat cgtattccag tgaccggtac
    35221 catgtgtccc gggcatgcgc atccatcaga ctcagcatcc cggaacgttg catcaggtca
    35281 cgccagattt cgcgatcgag attgcgtgtg atggctttca ttgccgtttc aggtttttcc
    35341 atgagccagc agccacagcg aaaatcctgt ttcatcgccc agtccagtgc ggttttaccg
    35401 ccaatacttt ctgtcattgc tgagatttca gccagttgcc ggataagtgt gtcgatttgc
    35461 agcagtgtac tctttcgtac agccagaatg tggcggatac tggttgagca aataacgtcc
    35521 gtatgcccgg tgaccacttc cggacgggag actgtgattg cagacataat cgatattcct
    35581 tttcgcagat aaatgaaaac gccagccctg ttcaggctgg cgtcagtgga atgaagtatg
    35641 gtgagtgaga ctcaccggtt ttgttgcaga aactgcagtt ggcgcaggat acggatatct
    35701 tccctggcct gttcaataca gggcaacagt gctgccgaat ccgttttatc ctcatcgttg
    35761 tcgaagataa tccctgattc gcagtcaata ttgtcctgca gccacgcaat cagaatatcc
    35821 agcgccgttt ctgtggttaa taatttcatc cagtgtcttt tgtcctgatt accagtcgaa
    35881 agtgggtaaa cccggcagac attctgcgcc ggcgtccaga tgaattagac tgacgccata
    35941 acgccgggtg agagtgacga ccagacgacg gaacgttctt gacaggccca ggcgtttaag
    36001 ccgcagaacc gggtatgacc acgcatcggt acgtaacaga tatccgctac ctgtgaaatg
    36061 aatccattct gattcaccaa cgtcactggt ctgatgcgac agcgagtata gccaggcgtt
    36121 gtccttttcc gtgatatgtg cggtactgca gcgtatgccg gcaagggccg taaacggtgg
    36181 tggtgtgcag gttgactgtt ggtcgaattc ctccaccacg cggagtgaat aaccgttttc
    36241 agcgacattg ttaatcagtt cagcgagatt aacaccatca atgtcaacga caatgcgccc
    36301 catgttcagc gcccgcacgt taacgctgtc agcttccagc gtcagggcca gtttcatcat
    36361 ttcgcctccg gatgcttacc ccgggtaatg ttatttaccg ttctgtaatt gtcgcgggtc
    36421 atcaggccgg ttgcccggcg agcccggagg atatcaatac tgtttattaa ctgagagcgg
    36481 ggacaagtgc tgaaccgggg ctggtcggta cgcaccagcg cgtatttttc aacgagaaag
    36541 ttcaccgcgt cacacagtga aatgcctgcc tcaatatgct gctcaatcac acgttcatcg
    36601 gagaacggtg tgtcattcag cgtaaggccg taatgctggt ccagcaggcg ggacagcagt
    36661 gtctgccaga tttcaacagg agacggacgc gaactggccg cctgcccggg taatacaggt
    36721 aatgttttca tactgaagat tttcctgata tgcagagatg aaaaatggga aagtggcgtg
    36781 gtgaaaacac caggccggag cagaaggtta ttctgatgat ttactttttc gtttcgggcg
    36841 tcggataaac agccatataa acgtaaccac aggagccgag ggtgtcggct tcgcaggtca
    36901 gtcctctggc atacagcgtg acggtatgct gatggcgggg attcagttca ccgctggtga
    36961 gtatgagttc cagttgtttc atcagcagcg gaaaggcctg gtccagatgg tacgcatccg
    37021 cgtcgctgaa ccggcctctg ataccggcgc ggtcggcaag gtaatacaac cggttaccct
    37081 cctgcaccag acgggagcca aaacagggtg ttatggtgca gggcagcccc caccaggggc
    37141 ggttgttata gtcgtcggga taatttgtct catggtgcgt gtctgacacg ataaagtcct
    37201 cacagaaaat cggttaagaa ggggttaacg atgacaggtc agccggtgtg gagaccggct
    37261 tcggtgaaga tgtattcatt gatgagaagg gcctcatcca cagcggcgtc tgaggtcatc
    37321 tggtcgtact cgttttccgg gtgacggaac attagttcag agtgacgctc atcagccgga
    37381 catacgggcc aaaactgtcc ttacggcgtt cagcaaacac ggccagcaca ccgggaatat
    37441 cctgcacttc acgaccggta tacgcttcag cactgccgtg ccagcggtat ttaccggtac
    37501 agaacggaaa aagacgggat gtcggatgtt gttggtgaat acgcatggct tcaccacggg
    37561 tgataatttt cataatagga tacctctgaa gacggaagat aaaagtgaaa acaggtgtga
    37621 tgtgattgtg gcggtgacgg gttaaagcag accgtgttcc gcaaaggaga aaacctggct
    37681 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    37741 gcgttccgtg ataaggcggt ctgccttact gggagtgact tcaccggacg ggtgattgtg
    37801 tgccagcacc acggctgcgg cattgtgata cagggcgcgt ttaatcactt cccggggatg
    37861 gacttctgtg cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg
    37921 gttattcaga tacagcaccc ggaattcttc acgctccagt cccgccatgt tcagaatcag
    37981 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    38041 cagggttttc agggcccgca gaatgagact gcgctcgccg ggcgtcatct ctccgggcag
    38101 aaaggaaagc tgctgcattg tccctgcctc cattcagtca atgatgcgca taatggcgtt
    38161 gcattccgga tgttgcaggg cgtaatcccg cagccggtaa tagtggaccg tcatggcata
    38221 acactccgta tgacaggcgt gatgactgta cgtcatcaga caggccgcaa tgccggcggc
    38281 ttccggactc atttcagcgc ggttaccgtt catggcattg aacagtaccc aggtttttcc
    38341 atcatcttca tccggttcgg gtgccataaa tgcgccaccg ttgttcaggg tgtacagatt
    38401 ccagatacca ccgcagtagt cttcgcacag acggtccatc cagccgaaga cacggggctc
    38461 cagggtcacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tacgttgttc
    38521 atcagggacg ggagtagcaa ccagttgggg ctggttattc cctggtgctg tggctgaaga
    38581 aagagtagtt gtgttctgag aaacgatttt catgagtgaa atccttacaa tgtgtataaa
    38641 tgagaggaaa taaaggccgg gagctggcaa catcacgttc atcactgacc gtgagacccg
    38701 gtaatatcag ggatgaatga tattcggctg aaattccgga cagccgtacg cgtaaccgtt
    38761 ccggtcacag acggatggcc aaatgccatt gcctgtactc agtctgcaca gactgagttc
    38821 tccgggacgt cgggtgattc tggtacctga atcacgcctt cagggatatg ttttatcagc
    38881 gcatgctctc cagcagcgtt tctgccatta cccacaatgc gcggttgagc ttaatatcgg
    38941 tgtcgatgct gtgaatggca cgggtgtgga tacgttttcc ttttgcactg cggccggaaa
    39001 tcccgccttt cagcatattc tcctgtatgg tctgataagc actccacagg tccttaccgt
    39061 aatcctcccg gcgtcgcggt gtcagaatat cagcggtggt gacgggctga tgctcattac
    39121 cataacggta agtcagtgcc gcctgtgcca gggcctggcg tgccggtggc ggcagaatca
    39181 gcgactgcat ggcatcacgc ttctcctcta tccggtcaaa aacgcccacc acctcgtaag
    39241 ccccttcaat aactttctcc actacgtctc cccggtgtgg aacacgcact tcccccagag
    39301 actgaccaca gacgcagccg ttctggcaga tgaacctgaa gtaacccggc agcatctggt
    39361 agctggaggt accgtcatgg gagttgagca gaataatttc agggacatgt tgtccgttta
    39421 tctctccggc ccgccgcaga cgcaacatgt gtttggtgta ttcccggcgg cccgggtcac
    39481 gtacgcgggt ctgacaggcg aagaagggct gaaagccctc ccgctgcaga ctttccagca
    39541 cggtgatggt ggggatatac gtatagcgtt cactgcggga ggtatgccgg tcttccccaa
    39601 aaataccggg tacataatgc atcagctctt catgtgtcag cggacggtca cggcgtatct
    39661 ggttcgcata accaaaacga cttgctaatc gcatgatctg ctccttatga atgattaagc
    39721 ggtataaacg aaaaagccgc atctcccgga gaagacgcgg cctgatggat gaaatgaatg
    39781 atgtttattg tctgaggtac ccttcacggg cgggccacgc attaagctgt tttccggcat
    39841 cagcttcaga gttcaggcag cgtcagcgtc gctgaccttc cgcgttgcgg ataacccgac
    39901 ggaaatgcat tcccgggtta tcctggataa acacattgcg gtaagcggtt atgacagcaa
    39961 ccgcctgccc gtatgagaaa gatccttcag ccaggacata ctctgtgtgt aacccggcat
    40021 atctggtttc tcctgataaa tagcctctgc catacgttgt ggcagaggct gaagcatgaa
    40081 actgacttca gggatcagtt aacatttttt ccggaaacgg taatcagcag tggatggtag
    40141 tcctggggat cgaaaaccga taacggcaga ctgacacgat ggccgttact ttcttcagtt
    40201 gctttaatga tttcggttgt ggcgacattt tccacgcact ccgtttccag aaatgcgtct
    40261 gtggctcgcg tggcattact gtcaccaaag gcttccgttt ccatttttct ggtcaccagc
    40321 gtctgaccat atttgtcttt gagttgcaga gtgatggtga gggggccaaa tccttcatcg
    40381 tttccgccat tatccagccg gaactggtaa gcacaaatat ttcccgggag ccatatcgta
    40441 tctgtattgc gtatactgat gtaacgttga tcctgtgccc ggagtggggc agaccacgtt
    40501 aaccccagaa tgaaggcggt aatcatgcag gttttgaaca ggtgaatcat ggtatttacc
    40561 tctctgagtc atgacgatga cactgacaaa tcaggtgata aagcgtaaaa ggcgcagaat
    40621 agccgttatg ccggtaactc cgggggtaat gcttcttcca gtcggttaac catattgccg
    40681 agatgggatg catcatattc catgacgggg cgttgcctga tgatactgac caccagtggt
    40741 ttgattaaca tattggtcgc ggcccgttgt tgtataccgg cggcgaaaat gatctccaga
    40801 gcattctgat cgataacacg ggccagattc gcgttcagat tttcaatctc atgtccggtc
    40861 agcaggaaac tgccggcaag ccatgtcacc tgctcctgaa tcattgccag aattttgacg
    40921 cgatcttctt caaccagcgc tccggcaatt tttcctgata cagcggaggc aatcattccc
    40981 cccagaacac cgccaaccac acgccctgtc agtgcaccaa atggtcccag tggactgaac
    41041 agtattccgc ccgccactga cccgacagca ccacctgcca cactggaaga tgccacggca
    41101 agattcctga tgaactgcgt accggagatc cgtccccgca acattttgat catgtcaggg
    41161 ccggttgtga cagctaccag tgccagagat gtcaccagcg tacccttcat cactttgttc
    41221 agggcactga tatttcctgc tccggtcccc ttctgaagag ccagtcggac agtcgggctg
    41281 gcagtcgaaa aatcaatgtg cttcagcata ccctgaacaa cactgagccg gtggagttgc
    41341 tgggttgtga cgtagacagc cagtgtgcgg gtgaaggttt ttcctgcctg gacagcagct
    41401 gtctgcaggg cagcatcgcg atcgccggtg ctgagccaga agaccgatgc cgtcagggca
    41461 aaactgatcc ctccggccgc cagactgaca accgaaccct cggcaatatc ataagtgacc
    41521 gattcgatgg tcccgaaccg ggtgatatta cgggcctgag tataagtcag atgtccccga
    41581 cgaatcagcc gggacgcttc tgcgggatcg gttacaccgg gtactttacc ttcgcggatc
    41641 ttattcctca tggtttcaac agcgccggca tactgatcac gggggacttc cagttgcatg
    41701 gggccattat ttcccatgta acgatactgt ccgttttgtc cgtcgaatgc cgcaccgacg
    41761 ctacgggctg cagttgaaca atatttcgtc tggatttctg ttccgctgac cagtctgtct
    41821 gctccatttc tggcattatt atcaccgagg atccgtgcgt ttttcagacg gactctgtcg
    41881 acaatatggt ttccccgttc agcggcaaac ccatgtccct gccctccgga agcaaacatt
    41941 ttctcatttg tactccaggt ttccggcagt ttcatgagaa tcagaccatt cgccgttgca
    42001 tcggaaataa agctgttgta agggcgttca taaatttcct gacacagtgt ttctgtttcg
    42061 cgacaaagag aggagcagta ccattccgga tccggcttat gattttccgg ggcatgatgt
    42121 acttctgtca gcccgcagga ggcacagata cgaagctcat ccacgtgatg gaatgataca
    42181 aaacggcgac cttctgaaaa gggataccag tcaccgccgt ccaggcaacg atcgagaaaa
    42241 tcgtcattcg cttccggtga taaattcagg cggttccgga agagcgtttt cagttcatca
    42301 ggtgtgaatg acaggttctc tttcaactgt tcgttaaacc ctgccatgtt tctttcatac
    42361 caggcattgt tcagtaacca gtttccgtct gccagttcaa attccctcag ctctccccgg
    42421 gaaatccgtt gagaaatgag cgtcagccag atgcgctccg tcagtgagtc aggaaggctg
    42481 taatcgtttt gcaaactggt cagccacgat ttactgttga gcaccggttg ttcttccagt
    42541 gtctggctga tggcacaggt cagttttttc agcgcctgga tttggctggc cgcccatagc
    42601 agcgtcacta atgcagccag ccagtacaac aacccgcccc accacatcag tgacggtgaa
    42661 atatcgtact gtaacgcgat caatggtgcg tatgcagaac acgccataag gaaaaaggca
    42721 gtataaaaac agcagtaata cccgttaatt tccttgctga tatcttctgg tgtgatcccg
    42781 ttctgctgaa tgtttttgat ctgcagggga acagccagca gaccaccggc gagagcggtg
    42841 gtcagaataa atttcacaga gtcaaaggtg tgagcctcca gcgcgatatg tttaccatca
    42901 agtccccaca tgaagctgat gatcattccg gcaatcgtca gaaatacgcc tgccagccag
    42961 cgaatatgga acaaccgggt tatgcgttcg gtgatatagc cagccagtaa ggccgggccg
    43021 aacagacaca gcaatatgag ccctgccagc gcagaagccg tgctgattaa tgctctgaac
    43081 aaaaggcgaa gaatggcaaa tcctgcgatc agcaggatca gcgcgccgac tatctgtaac
    43141 atagtcactc cgtgatgaat atcatgttat tgttaatgcc agcgaatgtg gcgttgaata
    43201 tccgttgcgc cggtccgggt cccggtcatg atgaccggga ccacagagag gcgatggttc
    43261 tgtcagaagg tcacattcag cgtggcctga ccgttatagc cttcagcgct gctgccgctg
    43321 acgctgtgga cataacctgc ctgaacgccc agggtgatat tttcccggac acgggcttcc
    43381 agtccggcct gcaggtccag tgacgtacca ttctgtgacg gtgagaacgt catgttactg
    43441 ccggctgcgg ctgtacccat gctcatgtct acccgggagc tgaaggtgcg gataacagaa
    43501 ggctgtaccc accagttcac cggcagttca cgcacactgt gtttggcact gccgcgcagg
    43561 gtgtcacggg atgaggtgcc tttaccaaag ttcatatcgt ggtggctgcc cagacggaag
    43621 ccggcacgca catgttgtgc actgccatgc ccgaacttca cataactggc gttatcctgg
    43681 ccgtcatcca gggagagtcc ctgccaggtg tactgcagtt gcggctccag catcagattg
    43741 tcagtgatac tgaagggcag accggtttcc agtgaaccca gccagccccg gccccggacg
    43801 cggaagtcgt tattgtccga tgaggctttc atgctgtggc gggttccctg ggccacaatg
    43861 tcagcccaca ggccggaggc gttgtgtatc agattcaggt atccgcccag gctgccggca
    43921 tcatcccgga ccgtgccggc acgggagccg tcatcatcct taacatcaac ggaagaatgg
    43981 cctgcagcac catacacccc tgtcgtcaga gacataccgg caacctctgt tctcatcagg
    44041 tcaccctcca gacggacgaa gccatagctg ccgctgcttt ccggcgtggc cccacggaca
    44101 ataccgccgt tgttatcgtg accgagatgg ccgccctgaa tgctgagacg gacgctgtta
    44161 ttttcaccgt ttacaccggt ctgatggctg cgggagcctg ccagaatccg gtcatagtcc
    44221 attgcctgtg tcagcatgga ggcatacagg gggacttctg cacgataagc attttcactg
    44281 cgcagatacc agctctcatc actgtcccgg ttgagggagt agttaaaggc cccggcctgc
    44341 agcctgttcc cctggacaaa ggccccttcc tccgtggtgg caccgttaat ggcttccacc
    44401 acctgaatac ccttaccgct ggtcgccagc cccgacgcac tgttgccggc gttcaccatg
    44461 ttcaggatgg tttttccggt tgccctgccg ccgtcaatga ccagtctgtc agcattgttc
    44521 tgtgccatat ccgggcgtac acgcaggctg atggtgccat tctgtccgtt caggtttttc
    44581 actttcaggg ttgccggtac gaacttccct gtgcgggtgg aggtgaaatg aatctgtccg
    44641 gcatggctga ggtcatccac caccgactgc accgtggcgt tatcgggaat attccaggtg
    44701 gcaccagagg cgagagtgac attcgtgggg tcaatggcac cgttcagcac agtgctgccg
    44761 gtcagcttca gggctgtacc gcgctgagca atgacatccg tggtgacggt actgtcgttc
    44821 agcgtcagcg tgccttcatt caggttgacg gctttctggg tgagtgtggt gttgctgaca
    44881 gtgagtgtgc cacttcctga tttttccacg ctgccgttac cggtgagaga gcctccctgc
    44941 aggagtgcat cgccttcacg gatggtcagg gtatcgttgt tcaccgtctt cccggaaagg
    45001 gtaagtatgg cgccgttatt cagcgtggtg gtgcccgcca gtgtgccgcc cctggcggtg
    45061 aacagtccgc catttaccgt ggtatccgtg gccgtatcac cggcgttcag cgtgaatgaa
    45121 ctgccttttt ccagcatcag ggcatccgcc tgaccacctc cgatactgaa tgcctttccg
    45181 tcgctccggg taccactgac agcggcaccg gaatcggcca gcagtacacc gccatttccc
    45241 atggatacgg tggtggcggt gccaccgttg cggacatcca gcgttccgcc atcatccacg
    45301 cgggtattag tggctgtgtg tccggtcagc acatccaggc gtccgccatt ttccagtacg
    45361 acattatcag ctttaccctc cacaacagag aatgctccca ggcggtttat gccggtaacg
    45421 gttgcagccg tactggtaac cagtgcgccg ccctgcttca gggtgacatt cgtggctgta
    45481 ccaccggcgt ccacctgcag tctgcccttc tgattaacgg tggtattccc ggccacaccc
    45541 ccgtttttga caatctgcca gccgtcactg tttacaacag tgccagacgc cgtaccgccg
    45601 ttgtgcacat actggtaccc cccattcagc gtggtatcca gtgcgtgacc atgtacagtc
    45661 tggtcgccgc cggcataaac cacagtggta tttgccgttc cttcagcagc cacaatctga
    45721 cgaccatttt tattgatggt ggtgcgtacg gcatctccgt agacggtctg cccggtatca
    45781 ccattttccg catccggtcc cccttccgcg ccggtattca caaccgtgtc ggttgccact
    45841 gcgccggatt tgatggcctg ccagcccttc tcattaatga cggtacccgt tgcaatcccg
    45901 ccttcatgta cccactgctc accgccgttc agagtggtgt tcactgcctg cccctgaagg
    45961 ctctgtccgc ctccggcact gataaccgtg tctgaaacgc ttcctccggc attcactctc
    46021 tgaagaccac caccggtgac agtagtgttg ttggcgatac cgccattttg tatccattgt
    46081 ccgccggtat tggcctcgtt atccggccca tactccagac cggtactgat ggtcattccg
    46141 ttggccgtac cgaggacaat ctggttgtca tgatttgtca gtgttccgtc gttcacggtt
    46201 tctcccgcct gtacaacctt gtcagcagcc agtgccggga ctgatgtgac agcagcaaga
    46261 gacagcgcaa ccgccacacc agcgcgtttt ccccgtgagc gggccagttc ggaggccacc
    46321 accagggtgc ccgtaatgtg attccatacc agcctgtagc tggtgttcag atgtcgtttc
    46381 atcagctttt ccttacagag ggtgaataaa aaagccggta cccacaaatg tggataccgg
    46441 caggtacaaa acagcatgct cagataaagt ggtttttccc ggacataaat gcttcccctc
    46501 tctcatccgc cagagaagta tcagtccgtt gtgtgcaatt ccgttctgca tgatgaagag
    46561 attcagctgc cttttcgcag accattaacc actataaacg atcgatagca acgatcgata
    46621 cctgttttat cgatcgtttt attctattaa cgacaaggcg gtcacgcaag agaaacagga
    46681 ggagtatgag gggtttgtgt catgttttca gttggttaac gtaatgttat caggcatttc
    46741 cctggcagac tgttcagaag aaaatccagt tccatacagc gcgggcaaca gagaccaccg
    46801 tgtcacggac ggcacgcaga accgtacggg caacagaggc aacacagacg ctctccgccg
    46861 tgtcaaatat ccggtccacc gcaccggtaa actgttcacg ggcctgagcg cggacagatt
    46921 ccgtgcgcag ctcgtcctgc aggcgggtca tcaggggact ggcggcatgg tcgggaagtg
    46981 ctgtcattag cgcactgacc agcgtatcca gttcccagcc ggtgcgggcc gatacggcaa
    47041 caaccggatg tacgggccgg aacagacgga ataccgcctc cgttttctcc cgaatgttct
    47101 gtgcctgtgc gggagaaggc tgaatgccgg ccatatccca ttcatggcag ggctccgttt
    47161 tgtcggcctg cgtcaccaca aacagcacct gctgatgtcc ccggtgcagg atgtgtcgcc
    47221 agaaatactc atccacagac agggcgcggt catcggcttt aatcagccac agcaccaggt
    47281 ccagttcggg cagaatgtca cggtacaggg cttcatactc tgcatccctg tcccggctct
    47341 cgcccacccc gggcaggtca gtgataacca tgctgtgacc gtgaccactc agacggaagc
    47401 gccgcacttc ccgggtgccg gcgtgaacat cactgaccgg ggtgacctcc ccctgaaaca
    47461 gtgcattaca gagtgaggat ttaccggccc cgcttttacc cataatgcca atcacgggtt
    47521 cgtgactggt gagtttgcgc agatgttcca ggatgtgacg ggaaagtgag taaggcaggg
    47581 aggagagcgg tttttcaatt gcctcaatgg catcagacgg attcatacat gtattcccgg
    47641 tgaaaaaaga caaaaccccg cgcaccggaa aggtggcggg ggattcacgg agataatatc
    47701 tgtctgaaat tgttttgaat cgatgggatt gttgcgtatt aacatgaagg agatcaggac
    47761 ttatccgtta ctgggtgatc tccttccatg aaaatacgcc gttattgtta aacagcaggc
    47821 tgatttctcc gtaaagggga aaatatttat cggctgcatg attatcaggt gtgtctgtgt
    47881 ttactgctgt gagtattttt cagtgtattg tgggggagat gtcagtggga gatattaaat
    47941 ttacgtttat aattatctta cttcttctgt gtgtttttta taagagtgca cacgtaatga
    48001 tatctgttga taccatgtgg ttaagtgttt cccttcctgg tcatgcgtaa tgattaaatg
    48061 cttttgtgac accattcttc cggtctgaat atttatcccg ttctttttta tttgtgcagt
    48121 gatactgtcg gaagagaatt cagtaatgtg aggtgtcctg gtcaggataa tatctgctga
    48181 gatattattg gttgagtgtg tttttactga gcaaaagctg gcttttaatt cctgtactga
    48241 aacgctgtcg ctcgtccggg gttaactgaa actggatgat gatgtaaaat gaaggctgtt
    48301 tttgtgtttg tcgaagaagt gttattttaa tccattctca tcattggctg gcgattatac
    48361 agaagtattt gaagaaaaaa tggtgcattt tatctgcgtc agcagggagg aagtatttta
    48421 ttgatgtatc agtattaaat ggtttttgtt atttataaat atgtcatcct gtccatagaa
    48481 tattgtactc agcattcagt gcaggaactg tagtcttctt tattttattc ttcttcagga
    48541 ggggggtgat tcctgaaggc gttattcccg gtgttctgtt tatgattctt cccggcagga
    48601 aaccggtatc tgcaataccc gggatgatgc tttttgttcg tacgtggttg tgtaaaattt
    48661 gaatagtgga tgtgctctga agggaaggag gggacacaca gattcatttt gtctttcttc
    48721 agcagcaaaa tacactgtgg ttattattta accattaaat caacaaaaaa ttttacctca
    48781 tattgattta actgctattt ttgctattaa tagccagaat tgcagtgctt ttatatctgt
    48841 gtatagtgag gttactatgt cattcggtca gccttgtgat gagtttcccc tgagttcgct
    48901 gcctccactc attcgtgatg ccgttattga agctcagcaa attacgcagg ctccccttgg
    48961 gcttgtggca gcgtctgccc tgggcgctgt ttcacttgtc tgccagaatc tgattgatgt
    49021 ctgcagactg aatacactgc gggggccggt ctctcttttt ttcctgacgc ttgccgagtc
    49081 cggagaacga aaaaccgcag tggacaaatt attaatgaag cccctgtacc agcaggagat
    49141 gcagttatat tccagataca aaagtgaact ggctgtctgg aaaaacaagg aagagctttt
    49201 aaaggctcag aaaaaagcat tgttgtcaaa actgaataaa gaactccgca aaggcgcgga
    49261 tgagtcagaa accctcagac aactggaagt acttcagaaa aacagtgcgg aagaacctgt
    49321 gaggtataaa ttcattttta atgatgccac gactgcggca attaagaatc agctttgtgg
    49381 taaatggcgt tcagtgggta ttatgtcaga tgaagccggt attatatttg atggatatac
    49441 cctgtctgag ttgcctttta ttaacaaaat gtgggatggc tctgtattgt ctgtggacag
    49501 aaagaatgaa cccgaacaga tgattgaaaa tgccagaatg acgttatccc tcatggtgca
    49561 acccggtctt tttgaccgat atatggagcg aaaaggttct gttgccaggg acagtggttt
    49621 tcttgcccgt tgtcttatca gtaaacctgc aaccacgcag gggaaacgct ttattaacgg
    49681 agctgtgatt ccgggaggat cattaacggc atttcatgaa cgattaatgg aactggccag
    49741 aggcagtatt gaaaaatcca gtgaggatga acggtattgt ctgcattttt cgcctgaagc
    49801 gcaaaaaata tttattgagc actataatgt tctggagcag gatttatccc cttccggacc
    49861 cttaagcccg tttcggggac atgtttcaaa aaaaacagaa aacattgcaa gaattgcagc
    49921 gttatttcag tatttcagct atggtgaagg gaaaatctct gccgatatca tgacgtctgc
    49981 tgtcgtaata agttcgtggt acacagatga atataaaaaa ttatttgcgt tacctgatga
    50041 atctgaactg cagcagaagg acgccgagga gctgttcgac tggctcattg aggagtgccg
    50101 cggagaatgc ccgccccgtg tcaggaaaaa ttatattctc cagtgtggcc cgggccggtt
    50161 cagaaacagg aaaaaactga acgcattgct gaacattctt gaatctcagt tcaggctttc
    50221 tgtcgtgccg gaaggaaaaa ccatgtatgt tctgcttccc cagatagcca gtttaaagtt
    50281 gtctgatgtc tcaggtattt ttacctcggg ttatcactac aataagctga gagcgaagta
    50341 accggtgagc agtttttctc cattatgctt cagacttaga tgattgatta ttatggggat
    50401 ttcatcaccg atattcagat gtatgaatat cggtgatgaa ctaaagctca ggagctgcgt
    50461 ggtggtgatg aatcaacagg catataactg atacccgccg gtcagaagtt ccagcctgcg
    50521 actttcctgt ctacgtgccc gcagtccggc gttcaggtgc agcgtgtgac agaacgcacg
    50581 gagagcgaaa cggcaggcag acaggccgtt cagtcagccc tggcaccctg cccggatacc
    50641 agcttccctg aaacaactgg catcaggtgc gcttatcgtc aaccgttgtg cggtatgcca
    50701 ggagcacgta ctgatccatc agatccgtca gtgctgcgct ctgcttctga cggttgatga
    50761 tccattcctc cagctctttt tctgatttcc gtttcagatt ctccgtccag tcgtcaaggc
    50821 agtttccggt cattaaatca agccagcaaa gccagaccag tttgaggcgc aactccgtgc
    50881 caccaaactg acggaagagg ctgatcagtt tatttgccag cgaacgctgc ctcagacgtt
    50941 cacattcaga caggagaatg ccttccagca tcggatgatg aaccagcaga tgttcactgg
    51001 tgcataaatt cactatcaga tcatcatcca ggggcggcgt ggtccgtttg tccggaccat
    51061 aaatcagccg gtgcagactc tggggtacga tggcaatctc cggtttttca ccattgaatg
    51121 acacccaccc gggcgtcaga ttatggaagt agtagatatt cggatgacac acgccccagg
    51181 atacggcgtg ccgggtgaag ccggtcagcc atttctggta gctttcgtgg agcagctcgt
    51241 aacgggtctg atactgtggt ttcattttca tattcctcac gggggataac aggcagcaga
    51301 acgccgccgg caggccggag actgcgtctg ttgcagatga acggttaaga attacggacg
    51361 ggactgtgca atacgcgcct gcagccaggc ttccacttcg cttttcagcc atcgggagct
    51421 gcggcccatt ttgatgggcg caggaaagtc accatccttg atgagtttgt aaaaccactt
    51481 atcggtcagg ccggtcagtt gagtgataaa tgccatgtcg accatctggt catccatcag
    51541 tgaaactggg gtgatcatga gaatgttcct ccggatgttg ttaaaccagc cggtgtggtc
    51601 atgaaaacaa cgcaccaccc tcaccggcat aacaaccggg gagaaacgta aaaaataatc
    51661 gggaatgtga ccgccaggag ataaacgggg aggttacggt ggctgagccg taccggtatg
    51721 ccgggtggtt gttacgaaac gatgcttccg cacgcgcgcg cgataaagaa gggatgattt
    51781 tcgtgaggaa gaggggaaaa ctggcgttct gataattgca gagttattta cggggcggtt
    51841 acggtggggc agcccgtgcc ttgtgggcag ggacaggtat cactggtggg ataaggctgt
    51901 ctgattggta ttatataaat ataagaagtt atcagagagt aacgtaaagg atttgccaga
    51961 ataacctaag agagtaaatc tctccggtaa cagagcgctg tgaagcattc tgtatcagag
    52021 tgcaaataca tgaacgctga tgcgtaaaat cagcagtgac acgccagctc cccctatgcc
    52081 cattcctgta taagacatga actgagcgta ttaatgctcc tgccctgaaa ctgagccggt
    52141 gagtgagggg ctgagaaatc cctcttgaca ctgctctaac cagaaatact tccccggttg
    52201 ttgcagtgcg tccggatccc cgataacgga acactaaggc tgtattgcgg gacaccaggt
    52261 aagtagcagc ccgtaatacg tcaggagacg cgggaaaagt gaattcccac acgcgttata
    52321 aacttaccct agcggagctg gaagccttta actctgccgt tgacaaccgg ctggcagaac
    52381 taaccatgaa caaactttac gatcgcgttc cggcttctgt ccggaaatat gtcacctgaa
    52441 cgttcggtaa tcacagcggt attgcgccag gtgttatctg ctctgtgtga ttctttttag
    52501 ggtttctcag attgttaaaa gctgaatatg ttcctgttgt cgggaaccag gataatacag
    52561 ggaacattaa gcggtatagc gacaacaaag cattcgaaat caaaagggaa tggtgtacgg
    52621 agttgtgggc agtaatgggc cgattttcgg gcattaccga tctctgttga ggttttctcg
    52681 tatttgccgg ggatccggtg cgttactgtc tggcacagca aacgcagagg tggcggggcg
    52741 ctctgctccg ctgattccgg ataatccctc ccctgtaacc tacggcaaca gtacaaatac
    52801 actgacgcct gaaacggtaa ccagaatcag caccagcagt aacgccaata aacagagcaa
    52861 acgatcggta tttcggtaat aaccgaaggg atcgacagag ccacaatacg gacaacacac
    52921 cgtattatga gtgatggtct taccacaatc cctgcacttc tttttgcgta tcaccataac
    52981 tccctcctca cagccttacc cgtaaccggt ttttgacatt aaaaaaacaa cgctgaaaat
    53041 tcaaccgtca gtccggagac gctcgttcgg gttatcacag agagcctgag acagtgtcct
    53101 gccggaggtc acttcaggga ttctgcgcat tttttacgac gtggctattc cgtatgaacc
    53161 atacggagat ttaaccatga cctacaaata caaccccttc tggcagcaac gtattcgtga
    53221 gacagtgcgg catgcactga atgttcatcc ccgcctgacg gcattgcggg ttgacctgcg
    53281 tctcccggat gtaccggcag caacggacgc agctgtgata tcccgcttca tcaatgccct
    53341 gaaagcccga atcgacgctt accagaagcg taagcgccgt gaaggtaaac gcgtgcatcc
    53401 cacaacactg cattacgcct gggcccggga gttcggggag ttaaaaggca aaaaacacta
    53461 tcacctgctg ctgctggtca accgggatac ctggtgtcgt gcaggtgatt accgcgatcc
    53521 ggggtcactg gccgggatga ttaaacaggc ctggtgcagt gccctgggtg tggataccgg
    53581 agatcatacc acgctggcgc attttccggt caggccggcg gtgtggctgg aacgcgatga
    53641 tgacaccggc tttcagcagg tgctggcgcg tgctgactat ctggcgaagg agagtaccaa
    53701 agcttacggc accggtgagc gtaattttgg ctgtagccgg agttgagcac gactggcgcg
    53761 ccaatacccg gcaactcaaa agtatgtgtt gttgtctggc ggcaacgatg tatccactcg
    53821 cccactgact gttttttacg tcagagttcc attgtgtgac aaccacagtg ctgtaacagc
    53881 aaccagcctg ttttacccaa actccagtcc agattttaac tgaaccgcca ttcacaccct
    53941 gagcacactg cccgtaccgg gacggctgtt gcctgcgtgt cgtctggcgc tgaggaatat
    54001 gacgatgtac gcaaaatcct ttattgcatt tgatggcaac ggacgtctga cgggcgcccg
    54061 tactgcacag gccgcacctt atgctcacta cacctgccac ctttgcggca gtgcactcag
    54121 ataccacccg caatacgaca ctgaacgtcc ctggtttgaa cacactgacg acgggctgac
    54181 tgagcacggt catgagtgcc cttatgtcag accggaacgc agagaaatac gtttgattaa
    54241 acgtctgcag caattcgtac cggatgcctt acccgtagta cgtaaagcca gctggtactg
    54301 cagacaatgt caccacgatt attatgggga gcagtactgc acacactgcc agaccgggcg
    54361 cttcagtgag gaggtggtag caggatgaac ggcataaaaa tggggctggg gatcacgccg
    54421 ggtgagcaca ttatcagtgc caactctgcg ctcagcagaa atatccggca ttgtttctgc
    54481 ctttcctgtc gtggccggtt gattttgcag acagatgctc agggggcgtg gtttgagcat
    54541 gatttacatg ccctgagcgc gcaacagaag gcggcctgcg tggtgctgaa tccggaaaag
    54601 tcccaccctt acattgagga tatggcgatg tttctatcgc cattaccggt cgtgctggag
    54661 tggcattgtg tgatgtgtga gcagtttttt catgggaaga aattctgtga ggcctgtggt
    54721 acggggattt attgcagagc ggtatgtacg aagtctgttt acaggtacca gccagatttg
    54781 taccaggact gcggaggtgc gagtacttaa tccaaaatat ccgctctgcg cagggcgttc
    54841 agaggtattg ccagcctgca ttttatccgg tgtttcgcac ctgccccgtt cgtgacacaa
    54901 aggcgcgaca ccggatatcc gcagtcctgc ttacctgaat aaaacccggg gtcatgattg
    54961 tgtgctcgtg gaacgaaatc gcaccgtgat cgcggattcg ccgaatctgc actgtggtgt
    55021 caataaacca cacgcgagag tcctctccgt attgatcagt gcatcaggac gatttctgca
    55081 ttgttaatcc agggggctgc attattcatt tccctgctaa tggacgttac atccgggata
    55141 ttcatgatcc tgatttctct gagcagtatc agcgcttatt ggagcgtctg gattatctga
    55201 agaagcccgg tactaaagca tcaggccagc gtaactctgg ctacagcggt ttttgacctg
    55261 ttgtaactgt aaggtgattt attatgtata cagaatcaaa cgttaagctt tcctgtaacg
    55321 ccctctggca aaagtgtgtc cttgcaactc tgtatcgggc tgcagccaca catcctgaaa
    55381 ttaaattatt acaatttaaa ttaataccag ggcagggtat taataaggcc attccctctc
    55441 tgatggctga ttttactggt gaactgcata gactcatttc tgaatatttg cagtgtaagc
    55501 gacgtaaaga taaatctgtt gagccccccc cctttatatt atctctggag cagggaggct
    55561 gtggctaacg ataccactcc ttatcttatg gtgctgctac ttaataacga tgtctggtgt
    55621 aagagtcgtg aggtggtggt tctcagaaat ctgattgagc aggcgtgggg aaaagtattg
    55681 ggtgaagata actccattac acatctggtt gtgtatcctg aagtgctgtc gttggtattt
    55741 gatgcagcta acacatacaa cgctgagcct ggtgtactgg aacagacagc ccgggcttat
    55801 attgaatcgc atggtgctgt tgaacgtcat cttgggtgta gtgactttta acgtcaggtg
    55861 acgcattgtc acttctgacc tgctacctgt acgccggaat ataatggtca gagaggcata
    55921 tttcgacgcc cctaatctgg ggcgtttgat tcaggatgtc tggaacaatc cgggctttgt
    55981 gtcaccgggc gcatcaaagc agtaccaccc gggatgtggc gctgttccct ggtgctggtc
    56041 cccccgtgaa tcttcctgcg tcccctccgg tcagccggca ctttgtcatc ctgactgatt
    56101 catcattcct ttcatattgc cagtcacagc tgtttgcatc gaaccgtact tgtgttgatg
    56161 agtctgcacc gacttaacca aattcaacag agataaaata gtgcaaataa ccgaagcctt
    56221 actttcagag ccgggggaaa tccggcgttt tgttcagcag gcggtggatc actggccaca
    56281 tctgctggca atccacttca cacttcattc gacagaaggc gtctgtggtc aacagattca
    56341 gacattctgt acttcctttt atgaacttat tactgtgcgt aatcacactg ccagcccatc
    56401 atcgccggtg gtattacgct ggttgcggga acaacatgga ggagcaacaa tacgatgcct
    56461 gttactgctc agccagacga gtgtttgcca tccgcgggcc agtgtcacag ttgatgaagc
    56521 atgttcgcaa gtggcggatt tactgcaaca gacctggccg gtgataagcg ctggcggaca
    56581 atgccgggtg gaaaggtgtt ttcgggttgt ccggcctgat acatcagagc aatacgttgc
    56641 gttaaaaacg gctgttcagt ctctgatgcc acggggtatt gcaacaatca tccggtgatg
    56701 gacgggagaa atatcgactc ttccgttagt ttttacagac tgataaagtt ttgccagctg
    56761 aatctttata cggaatactc ttcagtatgc atacacgaat tgactatctg gcggataaat
    56821 actgctttac agaactgaat gagtcttccc gtcttcgcct gcagtggcag gatgtcctgg
    56881 aggagtgccg gcagacagaa gccggaccag aagaacgact tcgcattgcc ctgttgaatg
    56941 tggattatgt caccagtttt gaactgcctt ttcgcttgtt gcttatccgt gcaccacaac
    57001 agattgccgc gctgcgtgaa gaatgggaca tcagccagaa aaatgtgatg ttcaacgata
    57061 aacgctttgg ctgcgtgtac agcctgaagg ccagcctttc cggtgtgccg gatacatacc
    57121 ggtatcatct gtctcatcgt attcgccggg tgattgggaa tgaaaacaca tcatcgccat
    57181 accagcagat tacccgggaa gtgaaagcgc cccgtgaacg gctgaagcat gcgctggagg
    57241 cggggttgct ggtgactgca ctggacggcc tgttctggtt cggcagtcag cgtattgcag
    57301 ctgatgtttt gaggctgaga aaggccggaa tgccggtggt gactacaacc gtggaggtac
    57361 acgataacct gacgggaacc acccgcaaaa taccggcata ccatctctga tacggccatc
    57421 agggggaatc ttgctctggt agctgcagta cgacattctt tatactttat ccccgtgcct
    57481 gaaaacatgt gctaaggaaa cgggaatggc aaggtccgat tatgatatta tcaatctgtc
    57541 tctggaacat gaactgaatg agtggctggc agagagaggt tatgccggac tggcggataa
    57601 ccggaatcgc ctggcagaag ttgttacccg caaattgcag gacagctttt atataaacgt
    57661 ttcctgggat gcgctgaaca cggcatacag tgaacacccg gagtggtttt ctgggcttgt
    57721 ctccggggat gagaattaat aaatggatta tgctgccatc aggcagtgtg attgccggca
    57781 gggatttcgt tttattctgt ttcttcccgg ggttcgggtt tgaattttcc cagaaagttg
    57841 tacatcggtc gcatgcaggg atgcggataa ccgtcccgca taaacaccac ccgggtttcc
    57901 tcaacactgc tcacggtgac aagcgcacca tgatcgtctt tgtatcgctg attgactttc
    57961 ggtaatggaa catcttctga cataaatttt agtacggtaa cagttaacta aattttcatg
    58021 tggtagcact gaatcaggag actgagtttc ctgatgtgcg atgttaattt tctgttttta
    58081 cgaaacattt tccccttctt cctgtatatt ttattgcgct cctcctttca tgtccttccc
    58141 atatcctgaa actcacttaa atgctgttga atcgtcacgc attgcaattt tcaatgactg
    58201 aaatcaaaaa atgatatttt tttagctaac caactgtatt aaaagcattc agtggaataa
    58261 ttttctgcac ttttccatta attatggtta taagttttgc gatatgctgt ttttgcgata
    58321 ttctgcagaa taaagatctg tttatttgtg ttatatcgat cgataaatct gtcatattgc
    58381 tgtagctcag atcattattc tataatgatc aaaattaagt caattctgaa cggatttgtt
    58441 actcagcatt ctggtaccgg ttggtgtggg taaataccgg aagggcggta gcgtaccact
    58501 gcaaaacatg gtgggaggta tcgtcaggag gctggtgatt gttggtatgg catgaataaa
    58561 aggtttttcg tttgactgga aaattatgtt gcaggaacga gagggctgag gaacaaagcg
    58621 tattccggcg ctgtccgtcc ttgctgtcac tgacactgat gtctgtttta ctatcgcctt
    58681 tatctcttca ggcagcggat atccggcgta gtggagagga tacatttatc attcagcagc
    58741 agcgtcagga agcccttgag caacaactga cgccttcagc ccctgatgtt cgcctttctg
    58801 cacctggctc ttttgcccat aagattaatt ttcctgttga aacgccctgt tttcagatta
    58861 aacagacgga actgaagggg gctgatgcgt taccacactg gctgccttta caaaaaatcg
    58921 ccaacggggc ggtcgggcat tgcctggggg cgaaaggaat taatctgctg atgagtacat
    58981 tgcagaaccg tctggtcgat catggttatg tcaccacccg tgttctggca ccttcgcagg
    59041 atttaaaaag cggtatcctc cggctggtta ttattcccgg tgttgtgcga catgtgcgtc
    59101 tgacaccgga cagtgatgac tatattcagt tgtattcctc attcccggca cacgaaggtt
    59161 ctctgctgga tttacgggac attgagcagg ggctggagaa tatgcaacga ctgccgggtg
    59221 tgcaggctga tatggaaatt gtgcccggtg agcagccagg ggaaagtgat attcttatta
    59281 cccgtaaaca gg
//
LOCUS       DAFATA010000017        58736 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFATA010000017 DAFATA010000000
VERSION     DAFATA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.017"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..387
                     /locus_tag="LGJ20_001429"
     CDS             <1..387
                     /locus_tag="LGJ20_001429"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Has polymerase, DNA-binding and 3'-5' exonuclease
                     activities. In Aeropyrum pernix this protein is sensitive
                     to aphidicolin and stable at 95#C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBK2385518.1"
                     /translation="IFRNEPYQEYIRETIDKLMAGELDARLVYRKRLRRPLSEYQRNV
                     PPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPLDYQRSPLDYEHYLTRQL
                     QPVAEGILPFIEDNFATLMTGQLGLF"
     gene            552..3458
                     /gene="rapA"
                     /locus_tag="LGJ20_001430"
     CDS             552..3458
                     /gene="rapA"
                     /locus_tag="LGJ20_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBK2385519.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            3470..4129
                     /gene="rluA"
                     /locus_tag="LGJ20_001431"
     CDS             3470..4129
                     /gene="rluA"
                     /locus_tag="LGJ20_001431"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBK2385520.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(4246..5061)
                     /gene="djlA"
                     /locus_tag="LGJ20_001432"
     CDS             complement(4246..5061)
                     /gene="djlA"
                     /locus_tag="LGJ20_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBK2385521.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            5316..7670
                     /gene="lptD"
                     /locus_tag="LGJ20_001433"
     CDS             5316..7670
                     /gene="lptD"
                     /locus_tag="LGJ20_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBK2385522.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYVVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            7723..9009
                     /gene="surA"
                     /locus_tag="LGJ20_001434"
     CDS             7723..9009
                     /gene="surA"
                     /locus_tag="LGJ20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBK2385523.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            9009..9998
                     /gene="pdxA"
                     /locus_tag="LGJ20_001435"
     CDS             9009..9998
                     /gene="pdxA"
                     /locus_tag="LGJ20_001435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBK2385524.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            9995..10816
                     /gene="rsmA"
                     /locus_tag="LGJ20_001436"
     CDS             9995..10816
                     /gene="rsmA"
                     /locus_tag="LGJ20_001436"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK2385525.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            10819..11196
                     /gene="apaG"
                     /locus_tag="LGJ20_001437"
     CDS             10819..11196
                     /gene="apaG"
                     /locus_tag="LGJ20_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBK2385526.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            11203..12045
                     /gene="apaH"
                     /locus_tag="LGJ20_001438"
     CDS             11203..12045
                     /gene="apaH"
                     /locus_tag="LGJ20_001438"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBK2385527.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(12123..12602)
                     /gene="folA"
                     /locus_tag="LGJ20_001439"
     CDS             complement(12123..12602)
                     /gene="folA"
                     /locus_tag="LGJ20_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBK2385528.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(12794..14656)
                     /gene="kefC"
                     /locus_tag="LGJ20_001440"
     CDS             complement(12794..14656)
                     /gene="kefC"
                     /locus_tag="LGJ20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBK2385529.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(14649..15179)
                     /gene="kefF"
                     /locus_tag="LGJ20_001441"
     CDS             complement(14649..15179)
                     /gene="kefF"
                     /locus_tag="LGJ20_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBK2385530.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(15287..16618)
                     /locus_tag="LGJ20_001442"
     CDS             complement(15287..16618)
                     /locus_tag="LGJ20_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2385531.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(16677..16964)
                     /gene="fixX"
                     /locus_tag="LGJ20_001443"
     CDS             complement(16677..16964)
                     /gene="fixX"
                     /locus_tag="LGJ20_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBK2385532.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(16961..18247)
                     /locus_tag="LGJ20_001444"
     CDS             complement(16961..18247)
                     /locus_tag="LGJ20_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK2385533.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(18298..19239)
                     /locus_tag="LGJ20_001445"
     CDS             complement(18298..19239)
                     /locus_tag="LGJ20_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBK2385534.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(19254..20024)
                     /locus_tag="LGJ20_001446"
     CDS             complement(19254..20024)
                     /locus_tag="LGJ20_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBK2385535.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLAADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            20498..22012
                     /gene="caiT"
                     /locus_tag="LGJ20_001447"
     CDS             20498..22012
                     /gene="caiT"
                     /locus_tag="LGJ20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBK2385536.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            22043..23185
                     /gene="caiA"
                     /locus_tag="LGJ20_001448"
     CDS             22043..23185
                     /gene="caiA"
                     /locus_tag="LGJ20_001448"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBK2385537.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            23314..24531
                     /gene="caiB"
                     /locus_tag="LGJ20_001449"
     CDS             23314..24531
                     /gene="caiB"
                     /locus_tag="LGJ20_001449"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBK2385538.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            24605..26158
                     /gene="caiC"
                     /locus_tag="LGJ20_001450"
     CDS             24605..26158
                     /gene="caiC"
                     /locus_tag="LGJ20_001450"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBK2385539.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            26267..27052
                     /gene="caiD"
                     /locus_tag="LGJ20_001451"
     CDS             26267..27052
                     /gene="caiD"
                     /locus_tag="LGJ20_001451"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBK2385540.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            27058..27648
                     /gene="caiE"
                     /locus_tag="LGJ20_001452"
     CDS             27058..27648
                     /gene="caiE"
                     /locus_tag="LGJ20_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBK2385541.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(27734..28129)
                     /gene="caiF"
                     /locus_tag="LGJ20_001453"
     CDS             complement(27734..28129)
                     /gene="caiF"
                     /locus_tag="LGJ20_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBK2385542.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(28390..31611)
                     /gene="carB"
                     /locus_tag="LGJ20_001454"
     CDS             complement(28390..31611)
                     /gene="carB"
                     /locus_tag="LGJ20_001454"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK2385543.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(31629..32777)
                     /gene="carA"
                     /locus_tag="LGJ20_001455"
     CDS             complement(31629..32777)
                     /gene="carA"
                     /locus_tag="LGJ20_001455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK2385544.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(33233..34054)
                     /gene="dapB"
                     /locus_tag="LGJ20_001456"
     CDS             complement(33233..34054)
                     /gene="dapB"
                     /locus_tag="LGJ20_001456"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK2385545.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSSKESGLFDMRDVLDLNSL"
     gene            complement(34221..35135)
                     /gene="rihC"
                     /locus_tag="LGJ20_001457"
     CDS             complement(34221..35135)
                     /gene="rihC"
                     /locus_tag="LGJ20_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBK2385546.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTLDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(35201..36151)
                     /gene="ispH"
                     /locus_tag="LGJ20_001458"
                     /gene_synonym="lytB"
     CDS             complement(35201..36151)
                     /gene="ispH"
                     /locus_tag="LGJ20_001458"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBK2385547.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAITLEGREENIVFEVPKELRVDIREVD"
     gene            complement(36153..36602)
                     /gene="fkpB"
                     /locus_tag="LGJ20_001459"
     CDS             complement(36153..36602)
                     /gene="fkpB"
                     /locus_tag="LGJ20_001459"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK2385548.1"
                     /translation="MSESVQSNSAVLVHFTLKLEDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(36690..37184)
                     /gene="lspA"
                     /locus_tag="LGJ20_001460"
     CDS             complement(36690..37184)
                     /gene="lspA"
                     /locus_tag="LGJ20_001460"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK2385549.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(37184..40000)
                     /gene="ileS"
                     /locus_tag="LGJ20_001461"
     CDS             complement(37184..40000)
                     /gene="ileS"
                     /locus_tag="LGJ20_001461"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK2385550.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(40043..40984)
                     /gene="ribF"
                     /locus_tag="LGJ20_001462"
     CDS             complement(40043..40984)
                     /gene="ribF"
                     /locus_tag="LGJ20_001462"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBK2385551.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(40992..41210)
                     /locus_tag="LGJ20_001463"
     CDS             complement(40992..41210)
                     /locus_tag="LGJ20_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBK2385552.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            41313..41576
                     /gene="rpsT"
                     /locus_tag="LGJ20_001464"
     CDS             41313..41576
                     /gene="rpsT"
                     /locus_tag="LGJ20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK2385553.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            42060..43481
                     /gene="espX1"
                     /locus_tag="LGJ20_001465"
     CDS             42060..43481
                     /gene="espX1"
                     /locus_tag="LGJ20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HBK2385554.1"
                     /translation="MTNGISTSPHCLYKSNIVDDVIINKTRQNELVKVFCEYKTEFLI
                     LFDDFFRSHNLPKPSPVLHHFFQYTHLRDAYFYRCKLIEHTVQFYFFKHKGITLLRLD
                     VFDDRTSECLSEEIKIYQECHEKFVNFLKANFNQEIYPQLYTPEIFYEACRNLQSFYD
                     HQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQ
                     NFKTSNIAYSQLCKKLSLFKSIPSRLIKKSAYCSTENTITNKFDVVFSYCGNNVKEFI
                     LLLPYNKSLEMHELNEQNIQYLTALNINIHKMLLSNITIEKSDLSYGYYFGCVLSNIL
                     CFESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLVNKHL
                     GYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSS
                     EMRKYLKSINECMFLMKNIAHQN"
     gene            43728..45059
                     /locus_tag="LGJ20_001466"
                     /pseudo
     CDS             43728..45059
                     /locus_tag="LGJ20_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            45072..46030
                     /locus_tag="LGJ20_001467"
                     /pseudo
     CDS             45072..46030
                     /locus_tag="LGJ20_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(46069..46968)
                     /gene="nhaR"
                     /locus_tag="LGJ20_001468"
     CDS             complement(46069..46968)
                     /gene="nhaR"
                     /locus_tag="LGJ20_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBK2385555.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(47034..48200)
                     /gene="nhaA"
                     /locus_tag="LGJ20_001469"
     CDS             complement(47034..48200)
                     /gene="nhaA"
                     /locus_tag="LGJ20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBK2385556.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            48786..48938
                     /gene="mokC"
                     /locus_tag="LGJ20_001470"
     CDS             48786..48938
                     /gene="mokC"
                     /locus_tag="LGJ20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBK2385557.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(49042..50172)
                     /gene="dnaJ"
                     /locus_tag="LGJ20_001471"
     CDS             complement(49042..50172)
                     /gene="dnaJ"
                     /locus_tag="LGJ20_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK2385558.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(50261..52177)
                     /gene="dnaK"
                     /locus_tag="LGJ20_001472"
     CDS             complement(50261..52177)
                     /gene="dnaK"
                     /locus_tag="LGJ20_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK2385559.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            52554..52958
                     /locus_tag="LGJ20_001473"
     CDS             52554..52958
                     /locus_tag="LGJ20_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBK2385560.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            52984..53697
                     /gene="msyB"
                     /locus_tag="LGJ20_001474"
     CDS             52984..53697
                     /gene="msyB"
                     /locus_tag="LGJ20_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBK2385561.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            53846..54412
                     /gene="satP"
                     /locus_tag="LGJ20_001475"
     CDS             53846..54412
                     /gene="satP"
                     /locus_tag="LGJ20_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBK2385562.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(54447..55034)
                     /gene="mog"
                     /locus_tag="LGJ20_001476"
     CDS             complement(54447..55034)
                     /gene="mog"
                     /locus_tag="LGJ20_001476"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBK2385563.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(55149..56102)
                     /gene="tal"
                     /locus_tag="LGJ20_001477"
     CDS             complement(55149..56102)
                     /gene="tal"
                     /locus_tag="LGJ20_001477"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBK2385564.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANSDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            56381..57811
                     /locus_tag="LGJ20_001478"
     CDS             56381..57811
                     /locus_tag="LGJ20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBK2385565.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLWMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            57881..58657
                     /gene="yaaA"
                     /locus_tag="LGJ20_001479"
     CDS             57881..58657
                     /gene="yaaA"
                     /locus_tag="LGJ20_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBK2385566.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT        14604 a        15448 c        14572 g        14112 t
ORIGIN      
        1 atcttccgta acgagccata tcaggaatac atccgcgaaa ccatcgacaa actgatggcg
       61 ggtgaactgg atgcgcggct ggtttaccgt aaacgccttc gccgtccgct gagcgaatat
      121 cagcgtaatg ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga aaaccaaaag
      181 cgcggtcgcc ccttgcaata tcagaatcgc ggcaccatta agtacgtatg gaccaccaac
      241 ggcccggagc cgctggacta ccaacgttca ccactggatt acgaacacta tctgacccgc
      301 cagctacaac ccgtggcgga gggaatactc ccttttattg aggataattt tgctacactt
      361 atgaccgggc aacttgggct attttgagca aaaaaaagag ttcgccagat accattttga
      421 tgcgtgacga atgctttgcc atccagtacc atagcgccct ttccattcct ggacctgaat
      481 aacgccacta cctcataagc acggtagcgg gtggttattg cctgcaatta aagatataga
      541 gccgaacaca tatgcctttt acacttggtc aacgctggat cagcgataca gaaagcgaat
      601 tgggacttgg aaccgttgtc gcggtggatg cgcgaactgt cactttactt ttcccatcta
      661 ctggtgaaaa ccgtctgtac gcacgcagtg attcccccgt gacccgcgtg atgttcaacc
      721 ctggtgatac cattaccagc catgacggct ggcagatgca agtcgaagaa gtaaaagaag
      781 aaaatggctt gctgacctat atcggtactc gcctggatac tgaagagtcc ggcgtagccc
      841 tgcgtgaagt tttccttgat agcaaactgg tgttcagcaa accgcaggac cgtctgtttg
      901 ccgggcagat tgaccgtatg gaccgctttg cgctgcgtta tcgcgcgcgt aagtattcca
      961 gcgaacagtt ccgtatgccg tacagcggcc tgcgcggtca gcgtaccagc ctgattccgc
     1021 atcagctcaa catcgctcat gatgttggtc gccgccacgc gccgcgcgtc ctgctggctg
     1081 acgaagtggg tttagggaaa accattgaag ccgggatgat cctgcatcag caactgctct
     1141 ctggcgctgc tgaacgtgtg ctgattatcg tcccggaaac cttacagcat cagtggctgg
     1201 tagaaatgct gcgccgtttc aacctgcgct ttgcgctatt tgatgatgag cgttatgccg
     1261 aagctcagca cgatgcttac aacccgtttg acaccgaaca gctggtgatt tgctcgctgg
     1321 attttgcccg tcgtagcaaa cagcgcctgg aacatctctg tgaagccgaa tgggacctgc
     1381 tggtggtcga tgaagcgcat cacctggtgt ggagcgaaga tgcgccaagc cgtgaatatc
     1441 aggccattga acaactggca gagcacgtgc cgggcgttct gctgctgacc gcgaccccgg
     1501 aacagctggg gatggaaagc cacttcgccc gtctgcgtct gctggacccg aaccgtttcc
     1561 acgattttgc gcagttcgtt gaagagcaga aaaattatcg tccggttgcg gacgccgttg
     1621 ccatgctgct ggcaggtaac aaactgagca atgacgaact gaacatgctc ggcgagatga
     1681 tcggcgagca ggatatcgag ccgctgttgc aggcagcaaa cagcgacagc gaagatgccc
     1741 agagcgcccg tcaggagctg gtttcgatgc tgatggatcg ccacggcacc agccgcgtgc
     1801 tgttccgtaa cacgcgtaac ggtgtgaaag gattcccgaa acgcgagctg cacaccatta
     1861 aactgccgct accgacgcag tatcagacgg ctattaaagt ctccggcatt atgggcgcac
     1921 gtaaaagtgc ggaagatcgt gctcgcgata tgctctaccc ggagcgtatt tatcaggaat
     1981 ttgaaggtga taacgccacc tggtggaact tcgatccgcg cgttgagtgg ctgatgggct
     2041 acctgaccag ccatcgctct cagaaagtgc tggtgatctg cgccaaagct gccactgcgc
     2101 tgcaactgga gcaggtactg cgcgaacgtg aaggtattcg cgctgcggtg ttccacgaag
     2161 gtatgtcgat tatcgaacgt gaccgcgctg ccgcctggtt tgccgaagaa gacaccggcg
     2221 cacaggtact gctgtgctca gaaatcggtt ctgaaggacg taacttccag ttcgccagcc
     2281 acatggtgat gtttgacctg ccattcaacc cggatctact ggagcagcgt attggtcgtc
     2341 tggatcgtat cggccaggcg cacgatattc agatccatgt gccttatctg gagaaaaccg
     2401 ctcagtcggt gctggtgcgc tggtatcacg aaggtctgga tgcatttgag cacacctgcc
     2461 cgaccggacg cactatttac gatagcgtat acaacgatct gattaactat ctggcttcac
     2521 cggatcaaac cgaaggcttt gacgatctga tcaaaaactg ccgcgagcaa catgaagcgc
     2581 tgaaagcaca gctggaacag ggtcgtgacc gcctgctgga aatccactcc aacggtggcg
     2641 aaaaagccca ggcactggca gaaagcattg aagagcagga tgacgatacc aacctgatcg
     2701 ccttcgccat gaacctgttc gatattatcg gtatcaatca ggacgatcgc ggcgacaaca
     2761 tgatcgtgct gacgccgtcc gatcatatgc tggtgccgga cttccctggc ctgtcggaag
     2821 atggcatcac catcaccttt gatcgtgaag tggcgctggc gcgtgaagat gcacagttta
     2881 ttacctggga gcatccgctg atccgcaacg gtctggatct gattctttct ggcgataccg
     2941 gtagcagcac gatttcactg ttaaaaaaca aagcgttgcc ggtaggtacg ctgttggtgg
     3001 aactgattta tgtggttgaa gcccaggctc cgaagcagtt gcagctcaac cgcttcctgc
     3061 caccgacgcc ggtacgtatg ctgctggata aaaacggcaa caacctggcg gcgcaggtag
     3121 agtttgaaac ctttaaccgc cagcttaacg cggttaaccg tcacaccggc agcaaactgg
     3181 ttaacgccgt gcagcaggat gttcacgcta tccttcaact gggtgaagcg cagatcgaga
     3241 aatctgcccg tgcattgatt gatgcagcgc gtaacgaagc cgacgaaaaa ctgtctgccg
     3301 agctgtctcg tctggaagct ctgcgtgcag tgaacccgaa cattcgtgac gacgaactga
     3361 ccgccattga gagcaaccgt cagcaggtaa tggaaagcct ggatcaggca ggttggcgtc
     3421 tggatgccct gcgtttgatc gttgtaacgc atcagtaacg gagccgaaaa tggggatgga
     3481 aaactacaat ccaccgcagg aaccctggtt ggttatcctg tatcaggatg accatattat
     3541 ggtggtcaac aagccgagcg gtttgttgtc agtgccgggt cgtctggaag agcacaaaga
     3601 cagcgtgatg acgcgcattc agcgtgatta tccgcaggca gaatcggtgc atcgtctgga
     3661 tatggctacc agcggcgtga ttgtagtggc gctgaccaaa gccgcggagc gggagttaaa
     3721 acgccagttc cgcgagcgcg agccgaaaaa gcagtatgtg gcccgcgtct gggggcatcc
     3781 atcccccgca gaaggtctgg tggatctgcc gctgatttgc gactggccaa accgcccgaa
     3841 acagaaagtc tgttacgaaa cgggtaaacc tgcgcagacg gaatatgaag tggtggagta
     3901 tgcggcggat aacacggcaa gagtggtgtt aaaaccgatt accgggcgtt cgcatcaatt
     3961 gcgtgtgcat atgctggcgc tggggcatcc gattctcggc gatcgttttt atgcatcacc
     4021 agaagcgaga gcgatggcac cgcgtttgtt gctacatgca gagatgttga cgattaccca
     4081 tccggcgtat ggcaatagta tgacgtttaa agcgccagcg gatttttaat cgtcacgctg
     4141 gcgcagttcg gtaggcggat aaggcgttca cgccgcatcc ggcaattgtg cgccgattgc
     4201 ctgatgcgac gcgggccgcg tcttatcagg cctacagttt aaaacttatt taaacccttt
     4261 ctgctgcttt atcagctcat atgcctgctg aatttcctgc gctttctgct tcgccatctc
     4321 catcatctca ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt cactcatcag
     4381 cttacggtag gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt
     4441 acaggcatct tccagcgttg ggccacgctg cgcttgctgc cagttaccac cgccagtttg
     4501 ctgctgataa ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa
     4561 ctgggcgcgg gagatcccta attcttcagc aatgacatac agcaccgccc gttcattcgg
     4621 gtgcagtgaa ccatcagcaa acgccgcctg aatctggatc tccagaaaca tacgaattaa
     4681 gtcaaaacga ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt
     4741 gtctgatttt cccacccgga acgcattttg cgccgcagta cgggaagcgc catgaagatt
     4801 cattcggtcc atcaactggc tggcgatatg aatatcagcc tccgtgacgc gacctttgga
     4861 tttggttaaa tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg
     4921 ctggttggcg aaccacgcca ttttacggct acgggcttta tcaaacatat ggccaattaa
     4981 caggcctaac actacgcccc aaaagccgcc gcccatcagt aaggccacgg ccacgccaat
     5041 gatttttccc caatactgca tatattcccc aaatcgacac acggatatca gggctatctc
     5101 ccacaatata aaggtgcttt taccgttttc cggctgcggt caattgtggg acatcgccta
     5161 taatttgcat tatcatacct gtcattcaat gccgtgccta acaccactga cgctattcgg
     5221 acaggattaa cactagcgta gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg
     5281 caacgttacc gatgatggaa caataaaatc aacgtatgaa aaaacgtatc cccactctcc
     5341 tggccaccat gattgccacc gccctttata gtcaacaggg actggcagcc gacctcgcct
     5401 cacagtgcat gttgggcgtg ccaagctatg accgtcctct ggtacagggc gataccaatg
     5461 acttacccgt gactatcaat gctgaccacg cgaaagggga ctacccggat gacgccgtgt
     5521 ttactggcag cgtggatatc atgcagggta acagccgtct gcaggccgac gaagtgcagc
     5581 tccatcaaaa agaggcacca ggacaaccgg agccggtacg taccgttgat gcgctcggta
     5641 atgtccatta cgacgataac caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga
     5701 acaccaaaga taccaacgtc tgggaaggtg attaccagat ggtgggtcgc cagggtcgcg
     5761 gtaaagcgga cctgatgaaa caacgtggcg aaaaccgcta taccattctg gataacggta
     5821 gctttacctc ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc gaaattattc
     5881 atgaccgcga agaacaagtt gcggagatct ggaacgcccg ctttaaggtg ggtccggtac
     5941 cgatctttta tagcccctat ttgcagttgc cggtgggtga caaacgtcgc tctggtttct
     6001 tgatcccgaa cgccaagtac accaccacca actactttga gttctacctg ccatattact
     6061 ggaacatcgc gccaaatatg gatgccacca tcacgccgca ttatatgcat cgtcgtggca
     6121 acatcatgtg ggagaacgaa ttccgctacc tctcccaggc gggcgctggc ttgatggaac
     6181 tggactatct gccttcagat aaagtctatg aagatgaaca cccgaacgat gacagttcac
     6241 gtcgttggtt attctactgg aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg
     6301 tcgactacac caaggtcagc gatcctagct acttcaatga tttcgataac aagtacggtt
     6361 ccagtactga cggctacgca acgcaaaaat tcagcgttgg ctatgtggtg caaaacttca
     6421 atgccaccgt ttcaaccaag cagttccagg ttttcagcga acagaacacc agtagctact
     6481 cggcagagcc gcagttagac gttaattact accagaatga tgttggtccg tttgatacgc
     6541 gtatttacgg ccaggcagtg cactttgtta acaccagaga cgacatgcct gaagcaaccc
     6601 gtgttcacct ggaaccgacc atcaatttgc cgctctctaa taactggggc agcatcaata
     6661 ccgaagcgaa gttgctggca acccattatc agcaaactaa tcttgactgg tataactcca
     6721 gaaacacgac caagctggac gaatccgtta accgcgtaat gccgcaattc aaagttgacg
     6781 gcaaaatggt ctttgaacgc gatatggaaa tgctggctcc gggttatacc caaacgctgg
     6841 aaccgcgcgc gcagtatttg tacgtgccgt atcgcgatca gagcgacatc tataactacg
     6901 actcatctct gctgcaatct gactactctg gcctgttccg ggaccggact tacggcggtc
     6961 ttgaccgtat tgcctccgct aaccaggtga cgaccggtgt cacatctcgc atatatgatg
     7021 atgctgccgt tgaacgtttt aatatttccg ttggtcaaat ctactatttc acggagtctc
     7081 gcactggcga tgacaacata acatgggaga atgacgacaa aacgggctca ctggtgtggg
     7141 caggcgatac ttactggcgt atctccgagc gttggggatt gcgtggcggg attcagtacg
     7201 atacacgtct ggataacgta gcgaccagta actccagcat tgaataccgt cgggatgaag
     7261 accgtctggt acagctgaat taccgttacg ccagcccgga atatattcag gctacgctgc
     7321 ctaagtacta ttccactgct gagcaatata agaatggtat ttcgcaggta ggtgctgtcg
     7381 ccagctggcc aattgccgat cgttggtcca ttgttggggc ctactactac gacaccaatg
     7441 cgaacaagca agccgactct atgttaggtg tgcaatacag ctcctgctgc tatgcaattc
     7501 gcgtcggtta cgagcggaag ctgaacggtt gggataacga taaacaacat gcggtatatg
     7561 acaacgcaat cggctttaac atcgaacttc gcggcctgag ctccaactac ggtctgggta
     7621 cgcaagagat gctgcgttcg aacattctgc cgtatcaaaa cactttgtga tttgttgatt
     7681 taccacgtaa tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg
     7741 ctgcttctcg gtatcgccat gatcgcgaat accagtttcg ctgcccccca ggtagtcgat
     7801 aaagtcgcag ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga tggattaatg
     7861 cagtcggtaa aactgaacgc tgctcaggca aggcagcaac ttcctgatga cgcgacgctg
     7921 cgccaccaaa tcatggaacg tttgatcatg gatcaaatca tcctgcagat ggggcagaaa
     7981 atgggagtga aaatctccga tgagcagctg gatcaggcga ttgctaacat cgcgaaacag
     8041 aacaacatga cgctggatca gatgcgcagc cgtctggctt acgatggact gaactacaac
     8101 acctatcgta accagatccg caaagagatg attatctctg aagtgcgtaa caacgaggtg
     8161 cgtcgtcgca tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac
     8221 caaaacgacg ccagcactga gctgaacctg agccacatcc tgatcccgct gccggaaaac
     8281 ccgacctctg atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg
     8341 cgtaacggcg ctgatttcgg taagctggcg attgctcatt ctgccgacca gcaggcgctg
     8401 aacggcggcc agatgggctg gggccgtatt caggagttac ccgggatctt cgcccaggca
     8461 ttaagcaccg cgaagaaagg cgacattgtt ggcccgattc gttccggcgt tggcttccat
     8521 attctgaaag ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac cgaagttcat
     8581 gctcgccata ttctgctgaa accgtcgccg atcatgactg acgaacaggc ccgtgtgaaa
     8641 ctggaacaga ttgctgctga tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa
     8701 gagttctcgc aggatccagg ttctgctaac cagggcggcg atctcggttg ggcaacagcg
     8761 gacatttttg atccggcttt ccgtgacgcc ctgacccgcc tgaacaaagg tcaaatgagt
     8821 gcaccggttc actcttcatt cggctggcat ttaatcgaac tgctggatac ccgtaatgtc
     8881 gataaaaccg acgctgcgca gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc
     8941 tcggaagaag cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg
     9001 agcaactaat ggttaaaacc caacgtgttg tgatcactcc cggcgagccc gccgggattg
     9061 gcccggactt agttgtccag cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg
     9121 ccgatgccac tctccttacc gaccgggcag cgatgctcgg tttgccgctc accctccgca
     9181 cttattcccc caactctcct gcacaaccgc aaactgcggg cacattaacg ctacttcctg
     9241 tcgcgctacg tgaatctgtc actgcggggc agttagcggt tgaaaatggg cattatgtgg
     9301 tggaaacgct ggcgcgagcg tgcgatggct gtctgaacgg tgaatttgct gcgctgatca
     9361 caggccccgt gcataaaggc gtcattaacg acgcaggcat tccgtttacc ggtcataccg
     9421 agtttttcga agagcgttcg caggcgaaaa aagtggtgat gatgctggcg accgaagaac
     9481 ttcgcgtggc gctggcaacg acgcatttac cgctgcgcga tatcgcagat gctatcaccc
     9541 ctgcgctttt gcacgaagtg attgctattt tgcatcacga tttgcggacc aaatttggta
     9601 ttgccgaacc gcgcattctg gtctgcgggc tgaatccaca cgcgggcgaa ggcggtcata
     9661 tgggtacgga agagatagac accattattc cgctgctcga cgagttgcgg gcgcagggga
     9721 tgaaacttaa cgggccgctg cctgccgata ccctgtttca gccgaaatat ctcgataacg
     9781 ccgacgccgt gctggcgatg taccacgatc agggtcttcc cgtgctaaaa taccagggct
     9841 tcgggcgcgg tgtgaacatt acgctgggcc tgccctttat tcgcacatca gtggaccacg
     9901 gcaccgcgct tgaactggcg ggacgtggcg aagccgatgt cggcagtttt attacggcgc
     9961 ttaatctcgc catcaaaatg attgttaaca cccaatgaat aatcgagtcc accagggcca
    10021 cttagcccgt aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga tcgacagcat
    10081 tgtttctgcc attaacccgc aaaagggcca ggcaatggtc gaaatcggcc ccggtctggc
    10141 ggcattgacc gagccggtcg gcgaacgtct ggaccagctg acggtcatcg aacttgaccg
    10201 cgatctggcg gcacgtctgc aaacgcatcc attcttaggc ccgaaactga cgatttatca
    10261 gcaggatgcg atgaccttta actttggcga actggccgag aaaatgggtc agccgctgcg
    10321 tgttttcggc aacctgcctt ataacatctc cacgccgttg atgttccatc tgtttagcta
    10381 tactgatgcc attgccgaca tgcactttat gttgcaaaaa gaggtggtga atcgtctggt
    10441 tgcaggaccg aacagcaaag cgtatggtcg attaagcgtc atggcgcaat actattgcaa
    10501 cgtgatccca gtgctggaag taccgccgtc agcctttaca ccaccaccca aagtggattc
    10561 cgccgtcgtg cgcctggttc ctcatgcaac gatgcctcac ccggttaaag atgttcgtgt
    10621 gttgagccgc atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct
    10681 cggcaacctg tttagcgtcg aggtgttaac gggaatgggg atcgacccgg cgatgcgagc
    10741 ggaaaatatc tctgtcgcgc aatattgcca gatggcgaac tatctggcgg agaacgcgcc
    10801 tttgcaggag agttaacgat gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc
    10861 tacattgagg cacaatcttc acctgataat gaacgttacg tttttgctta taccgtaacc
    10921 atacgcaatc tggggcgagc gccagtgcag ttgttggggc gttactggct gatcaccaat
    10981 ggcaatggcc gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca gccacttatc
    11041 gcgcctggcg aagagtacca gtacaccagc ggtgcaatca ttgaaacccc gctgggcacc
    11101 atgcagggtc actacgaaat gatcgatgaa aatggcgtcc ctttcagcat cgacattccc
    11161 gtattccgac tcgccgttcc cacactcatt cattaaaaga atatggcgac ataccttatt
    11221 ggcgacgttc atggttgtta cgatgaactg atcgcattgc tgcataaagt agaatttacc
    11281 cctgggaaag ataccctctg gctgacgggc gatctggtcg cgcgcgggcc gggttcgctg
    11341 gatgttctgc gctatgtgaa atccttaggc gacagcgtac gtctggtgct gggcaatcac
    11401 gatctgcatc tgctggcggt atttgccggg atcagccgca ataaaccgaa agatcgcctg
    11461 acaccgctgc tggaagcgcc ggatgccgac gagctgctta actggctgcg tcgccagcct
    11521 ttgctgcaaa tcgacgaaga gaaaaagttg gtgatggccc acgcagggat cacgccgcag
    11581 tgggatctac agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct atcgagtgac
    11641 tcctatccct tctttctcga cgctatgtac ggcgatatgc caaataactg gtcaccggaa
    11701 ttgcgggggc tgggaagact gcgttttatc accaacgcct ttacccgtat gcgtttttgc
    11761 tttccaaacg gtcaactgga tatgtacagc aaagaatcgc cggaagaagc ccctgcccca
    11821 ctgaaaccgt ggtttgcgat tcctggccct gtcgctgaag aatacagcat cgcctttggt
    11881 cactgggcat cgctggaagg caaaggtacg ccggaaggta tttacgcgct ggataccggc
    11941 tgctgctggg gtgggtcatt aacctgcctg cgctgggaag ataagcagta ttttgtccag
    12001 ccgtcgaacc ggcataagga tttgggcgaa gcggcggcgt cttaaacaca gcctgatata
    12061 ggaaggccgg ataagacgcg accggcgtcg catccggcac tagccgtaaa ttctatacaa
    12121 aattaccgcc gctccagaat ctcaaagcaa tagctgtgag agttctgcgc atcagcatcg
    12181 tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa atgggtgtcg
    12241 ccttccactt ctgcgtcgat atgcgtcaga tacagttttt gcgcttttgg caggaactgc
    12301 tcataaacgc gaccgccgcc aatcaccatg atttctggta cgtcaccaca cgccgcgatg
    12361 gcttcatcca ccgacttcac ccacgttacg cgatcgtccg tacccggttg actgctgagg
    12421 ataatatttt tgcgtcctgg caacggacga ccgattgatt cccaggtatg gcggcccata
    12481 atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg caggttccac
    12541 ggcatggcgt tttccatgcc gataacgcga tctaccgcta acgccgcaat cagactgatc
    12601 attgagattt cccgataaaa aaaattgtcg ccactatacg taaagcgtaa accgtcgtcg
    12661 actggtgcga ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc cacgctctct
    12721 ccctggactc gccgcattac aatgaaacaa aaacaaacag ttagctgtaa agtgtgattt
    12781 acgtcactct ttattaggat gagggtttcg tttccggttc atccgccatg ttgccggtat
    12841 gtttaccttc ttcggttccc tgccagccat gtcgttgaat taatgacaga tgttcgcggt
    12901 cctcggtaat gatctcactt aacatcgcgc tggtgcgttt atagaccgcc gcgcgggctt
    12961 tggtgtcgtt ctcaaccatt gccatctctt ccaccatctg aatattaaag cggcggaaca
    13021 catcggcacg ttctcgcgct tcatacggcc ccagacctaa actttccagt gccagacgcc
    13081 cggttttcag cgcaccttcg aaggtttcac gctccggctt ttcaacgcct gcctgacgca
    13141 aacgaatgta gtggtcgaca tcgcgggcgc gggcaataat ctgcaaatgc gggaaatgtt
    13201 ctttcaccat ctctgtcagt tgcaggttgg tttgcggatc gtcgatggcg ttaatcagca
    13261 cttccgcttt cgccgctccg gcagattcca gtaaatccat ccgcgtggca tcgccataaa
    13321 acactttcat accaaattta cgcaaggttt cgatatggtc cggatcgtga tcgagtacca
    13381 ccattttcac cccgctggag agcagtaaac gtccggtaat ctgcccaaaa cgaccgaatc
    13441 cggcgataat cacgcgcggc tgttcttcgt cgatctcatc ggcttcacgc gcttcctcag
    13501 tagaagattg ctcaaggcga ttgaggatca ccagcagaat cggcgttgct gccatcgaca
    13561 gcgccaccgc cagggtcagc gatttcgccc actccggctc cagcacattc gccatctgcg
    13621 ccgcgccaaa taccacaaag gcaaactcac tgccctgccc taacaacacc gcaaaccaac
    13681 gacgctgttt atttggcact tgcaacggtc gggcaatcag ccacagcatg gcgattttga
    13741 tgatgaggaa accgagcagc aaaatgacaa tgcgcaatgg gttttcaagc agcgtgccaa
    13801 agtctatcga catgccaaca ccgatgaaaa acagccccaa cagcaaacct ttaaatggtt
    13861 cgatatcgct ctccagcgca tgacggtatt ccgaacttgc cagcagtacg cccgccagaa
    13921 acgcgcccat cgccatcgac aagccgacct cttccagcag caaaccaaag ccaaacacga
    13981 ggaataacgc cacggcacta aacacttccc gcaagccaga gcgggctaca aaacgcagcg
    14041 ccggacgcgt gacatagcgc cccagcaata ccaccagcac cagcgcaccc gccactttta
    14101 acgccgagag agcaaatgcg cccatcgtcg tcgaggcact gctcgttgcc agtagcggaa
    14161 tcatcgccac cagcgggatc gccgcgatat cctggaacag cagcaccgca aaggcactgc
    14221 gacccatttg cgtcaccatc agattgcgtt cattcatcgc ctgcatggca atcgccgtag
    14281 aggagagcgc cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc ccaagtaaca
    14341 tgcagaacag ccccagcagg ccgccgcaaa tcaccatctg caatgcgccg ccgccgaaca
    14401 ctgccgcacg cagcttccat agcctttgtg gatcgagttc gaggccgata ataaacagca
    14461 tcagcaccac gccaatctcg gcaaagtgca ggatagattc ggcatcggtc accagtcgca
    14521 gcccccacgg gccaataatg cagccggcaa tcaggtagcc aagtaccgat cccagaccaa
    14581 gacgtaccgc aatgggtaca atcagcgccg ccgatccgag gtagatcagc gcctgaacca
    14641 gcgtatggct atccatgatg ggcctcctgc cattccagca gacgttgctt atagtgacgc
    14701 gcctgccctt cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc aaacggtggc
    14761 agccagttca gcccgcagta gattgccgtc gcctgtagcg gctgcgacag cacatcaaag
    14821 cccggatgcg caccaatttc aaaatggctt tccccgccgc cggtcgtcac cgcccacagc
    14881 aaatgtttgc catgcagcgc cgtgccgccg tgaccgtaag cccagccgtg cgagaaaact
    14941 ttatcgatcc aaagtttgag gagcggtgga atgctgtacc actgcatcgg gtgctgccag
    15001 acgatcagat cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat attgaagtca
    15061 ggatagagtt gataaagaga gcgaatttcg acgccttcca gcgtccttgc ctgttcaagc
    15121 atccgtttat tcgcatggga atgatgcgga tacggatgcg cataaattat aagaatcata
    15181 ggtagcccgc gtgattacgc ctttgttttc ccacaaagtg tagtcatata ttcacgcggc
    15241 ttatagtcaa taatattgat tcagaaaaga gagaaatctg aacaatttac cccattctct
    15301 gcccgcggat cgtcatattg ctggtttgcg ccagtgacat ccctcgagtc tccggggcaa
    15361 acgctacgga aatcaacaag ccaaacagcg agatacccgc ccccattagc atcgtgttac
    15421 tgataccgta attattgata aagatcggca gtgcccaggt cgaaacaatg gtgccaatac
    15481 ggcttaagga cataatcacg cccacggcag aggcgcggat atctgtcggg aagagttcat
    15541 taggatagag ccactgcaaa ttacccggcc cgccagagaa aaaggcatac accgcaaagg
    15601 ccatcactac cagccagatc cccatatccg ggatcagccc caaaaccgcc agcgccagcg
    15661 tcatcatggc aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt agccacagca
    15721 tcggcggaat acagccgagc ataaagaaca ggctaatcac cacgttcccc agtgccgcgt
    15781 ttttgccaac ccccaatccc aacaaaccaa cgatttgcgg gccaaaggtg taaatggcga
    15841 acattgggat cacctggcag gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc
    15901 ggcgattaaa cagatcgcga aaacgggttt gctgcggctg ctcttcatcg aaagccaccg
    15961 gttcgccaaa cagttttatc atcatctctt cgcactcttt tactcgccct ttgcgtaata
    16021 accagcgggg agattcaggc agttcgaatc gaccaatcaa aatcaacaaa caggggatcg
    16081 ccgcgctacc cagcatccag cgccagccgc cttccacatc ataaagccag taaccgacca
    16141 gatcggcgca ggtcgcgccg acataccaca tggcggcgat aaagctgatg gaaaacgccc
    16201 gctgacgggt actggagaac tcggtgatca ttgaggtggc gatgggataa tctgcaccga
    16261 tgacaatgcc gataagtacc cgcatcacca acagttcgac aggggatgag acaaacatcg
    16321 tcgccaccga tatcacgccg atggcgatga tatcaatgag gaacattttg cgccgtccga
    16381 ctttatcgga aatatagcca aacagcgatg tgccaacgaa cagcccggcg agcgttcccg
    16441 cgcccaacaa gccaatccag tcagcgtcca gttttagcgc cggagtcagt tgctccagcg
    16501 ccacgccaat cattaccagt acataaccat ccagaaacgg accaccgctt ccccacagca
    16561 aaatgcggcg gtgaattgag gagaatttga gatcgtcaaa gtttctggac ggttgcatgg
    16621 gttattcctg tttttttgca atgagagtca gtacggcgtt acgtatcaaa ccaacatcag
    16681 ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc cagcgccgac
    16741 cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc gaagcgcaca
    16801 ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag cagctccagg
    16861 gcctgtttat cggcatcggc cttcacaaca atgtgcggat gctcttcatc gacattgaat
    16921 ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg gtcactcctt
    16981 tcatgccatc cttgatcaga ttgatgaagc ccactttctt gccgtggcgg aggattttct
    17041 tgcgcatcag ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc acgcccaccg
    17101 ccagttccgg gtagccgcta aacatgcgtg ggttatcaag gaaggccggt agtttctggt
    17161 acatacgcat atcgcgcagc gggccactct caagatgctg acgatattcc gccagttttt
    17221 gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt ttttgctgcg gcttccccgg
    17281 cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac attccggcgg
    17341 catcaccggc aatcaatacg ccgtcaccaa ccaactccgg cagcatgttg atgcctgctt
    17401 ccggcactac gtgagcggaa tattccacca gcttgccacc cgcgatcagc ggtgcaacgg
    17461 ccggatgttg tttgaaatct tccagcattt gcggcaccga tttttttgcg tcatgcagat
    17521 gatgcagacc acaaaccagc cccagcgaca gggtattttc attggtataa aggaagccgc
    17581 cgcccatcag gccatcggtg ggtgatcccg caaacagaca agccgcgccc tgattaccct
    17641 gcaactgaaa acggtcttcg attaccgact tcggtaactc gatcagttcc ttcacgccaa
    17701 ccgccacatc cgtcggttta acgcgttttg ccatccccag cttttcggca aggatggagt
    17761 tcactccatc agcaaggatc accgttttcg cttcaatcac atcgccatcg gcttctacac
    17821 cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta attaactgcg
    17881 cgcccgcttc ttcggcctgc tccatcagcc aggcatcaaa tttactgcgc aaaacggagt
    17941 aagaacgctg ggatggcgag gtttcgtcac cattgcagta gtccatagtc atcgctgact
    18001 tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg gcggagtcgg
    18061 cgaaaccagg aataatgtgt tccaggctgt gggcatagag acgcccgccg gtgacgttct
    18121 tggcacctgc ggaattgcca cgctcgataa ctaacacttg cgccccttcg cgggcgagca
    18181 ccagtgcggc aaccgaaccg gcaagcccag caccgacgat gatggcgtca aagatatctt
    18241 cggacataag agctccctgt cagcggcagg gccaaaatgc agccctgcca gagtggatca
    18301 acgcgctaaa gctgcggtca gcgccggaag gatcttcacg gcgtcaccaa caatgccgta
    18361 atccgcgtac tggaagatcg gcgcattttt atctttattg atggcgaaaa tggtttgcga
    18421 tgcattagca ccaaccatgt gctggatctg cccggagatc cccaccgcca ggtacagttc
    18481 aggtttcagc atcaggttgg agataccgac atagcgttcg tgctccatcc atttttcgtt
    18541 ttccgccacc ggacgagaac aggccaactc cgcacctatc gccttgcaaa gctgttctgc
    18601 cagcgcaatg ttctctttgc tgccaatacc gcgaccgacg ctgaccacca gacgggcttt
    18661 gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc gtgcgggtaa tcgccacagc
    18721 cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg gctgagccgc
    18781 atcgaacgtg ccgctgctga tggtcagtac cgcatacggc gtggcaatgc gttcttcgcc
    18841 aatcgccaga ccaccgtaaa ccatgtgttt cactgtcgct ttaccgtcct gtacgctgac
    18901 ggtgctggca tcgttagaca ccgccgcttt aaggcgataa cccagttttg ccgccagtaa
    18961 tttgccgcga cgggtgtttg gcagcagcac caggccgtct gcgccgtgct ggcgaatagt
    19021 gtcagccatg acaccggcgt aatcttcgat catccgatcg tccggtttgc cgtttaattt
    19081 ccagacatga ttagcgccga gctggattgc ctgtgcgccg tcggcatcat tgaggacaaa
    19141 ggtgttgatt tgattagcta aagcctgcgc accgttcatc agttccggca gacgagaagg
    19201 ggtatcgctg aatacccaga cttgagaaaa cgtgttcata gcatcccctg taattaaatg
    19261 actttgcgaa gattttcagc aaatgcggcg atctgttctt cgccgtcgcc ttcaatcacg
    19321 atgcgctgac gttcgcgctg tttcggcgcg gcaacctgtt gttctgacca ggctgcctct
    19381 gcgttaaaac caatatccgc cgccgaccat acctggacgg gctttttcgc cgcgccgaga
    19441 atggctttca tcgaaggaat ttgtggggaa ttgatatcag tggaaacagc aacaaccgca
    19501 ggcagcggaa tgcttaaggt ttcggtttca tcttccagtt cgcgctcaac ggtgagggta
    19561 tctgcagcca gggagataat tttgctgacg ccgttaactg ccggaatatt gaggatttcg
    19621 cccaccagca gaccaacctg ctgggcataa aggtcggaag aaccatcgcc acagaggatc
    19681 aaatcaaagc ctgctttctg ggcggctgca gccagtgcgc tcgccgtttg ttgcggcagt
    19741 gcctgctcga actggtcatc aatcaccaca atcagttcat ccgggccgcg cgatagcaca
    19801 tctttacgcc ctttggcgtt ggtcagggct ttaccgccca cacttaaggc cgtcacctgc
    19861 gcctctgctg cctgttgctt tagctggcaa gccgcttcaa tagcgttcag atcgtattgg
    19921 cttattttgg catcggcttt gctgaagtct aatgaaccat cagcattatt gaccgcaata
    19981 tcctgttcat caggcacgca cttatagcaa gtaataatct tcattgcatc tccagaaatc
    20041 atgaaggaaa cgtcgttcat atcggtggtg ttaattacag atatcacgcc ctttaatttg
    20101 gaaataaaac tttaatcacc aatattgaaa atgtcacacg cagataaatc caactttcaa
    20161 tattgttaag ttcctcacca atattgaaaa catggcgtag caaaaagaaa ttttcaatat
    20221 tgctttatgg atcaccaata ttgaaagtca gatgctattt ttttactcac aacacaacat
    20281 aacaaactga ttaataatca aaattaacga gaaaagcgcc ctctattcac ccgaaacaaa
    20341 aatgtgataa caatcacaga atgcagctta ttgaataccc attatgagtt agccattaac
    20401 gcgtccacga ggttaataat aattatatta aatgttaaca aaaataaaac aaacgggaac
    20461 gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa agagaaaaac
    20521 gggaatagaa ccgaaggttt tctttccgcc gttaataatc gtcggcatac tttgttggct
    20581 tacagtcaga gatctggatg cagcgaatgt cgttattaat gctgtattca gttacgtcac
    20641 caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg gttggttctg
    20701 gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccacctg aatttagtac
    20761 cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac tgttctgggg
    20821 atcgattgag atctactact acatctccac cccgccgttt ggcttagaac cgaactcgac
    20881 aggggcgaaa gagttggggc tggcttacag cttgttccac tggggacctc tgccgtgggc
    20941 cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca aaatggaagt
    21001 gattcgcccc agctcgacac tggtgccgct ggtaggtgaa aaacacgcca aagggttgtt
    21061 cggcactatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg gtaccagtct
    21121 gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca ttccgcatac
    21181 cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca tttgcgtcgc
    21241 ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc tgagcttcct
    21301 gatgctgggt tgggtgttca ttgtcagcgg tgccagcttc atcatgaact acttcaccga
    21361 ttcggtgggg atgttgctga tgtatctgcc gcgaatgttg ttctataccg atcccatcgc
    21421 taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg tgatttacgc
    21481 catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc gtgaactgtg
    21541 cttcggcatg gtgatggggc tgacagcatc cacctggatc ctgtggaccg tgctcggtag
    21601 taacactctg ctgttgatgg ataaaaacat catcaacatt ccaaatctga tcgaacagta
    21661 cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca ccgccaccat
    21721 gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg cctgctctta
    21781 taccctggcg atgtccactt gccgcgaagt acgcgatggt gaagaaccac cgctgctggt
    21841 gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg cgctcggcgg
    21901 cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct tcgtcaacat
    21961 tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt aattaacccc
    22021 caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg aactgtttgt
    22081 cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg ccgagtgcga
    22141 ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg gtatcgacag
    22201 tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc tcgccgccgt
    22261 gtggatggag ctgggacgtc tgggggcacc aacctatgta ctgtaccagt tgccgggcgg
    22321 gttcaacacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta tggctttccg
    22381 cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg gctccgacgt
    22441 gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta atggtagtaa
    22501 gtgttttatt accagcagcg cctacacccc gtacatcgtg gtgatggcgc gcgacggggc
    22561 ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac cgggcatcaa
    22621 agtgaccaaa cttgaaaagc tcggtctgcg tatggatagc tgctgtgaaa tcacctttga
    22681 cgacgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct ttaaccgcgt
    22741 caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg gtacggcgat
    22801 gtgcgccttt gaagatgcgg cgcgctacgc caaccagcgc gtgcagtttg gcgaggctat
    22861 tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat taaactccat
    22921 gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca cctctggcga
    22981 tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg atagcgcaat
    23041 gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct tctggcgtga
    23101 cctgcgtgta gaccgcgtct ccgggggatc tgacgaaatg cagatcctga cgctgggtcg
    23161 tgcggtgctg aagcaatacc gctaagtcaa tgcgctgcct gatgcgacgc tgatgcgtct
    23221 tatcaggcct acgggagcac gaatgtaggc cggataaagc gtttacgccg catccggcag
    23281 tcatgcgcta cataaatttt tcaggagcaa ataatggatc atctacccat gccgaaattc
    23341 gggccgttgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg accgtttgcc
    23401 gggcaaatgt tcgcagaatg gggcgcggaa gttatctgga tcgagaacgt cgcctgggcc
    23461 gacaccattc gcgttcaacc gaactacccg caactctccc gccgcaattt gcacgcgctg
    23521 tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat ggaaaccacc
    23581 gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat taccgatgaa
    23641 gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt tggtcagtac
    23701 ggcaccgagg agtacaccaa tcttccggcc tataacacta tcgcccaggc ctttagtggt
    23761 tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta taccgccgat
    23821 tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa agtgcgtgaa
    23881 accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg tatgggccag
    23941 tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag caaaggtaaa
    24001 gatccctact acgccggttg cggtctgtat aaatgcgccg acggctacat cgtgatggaa
    24061 ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc acatctactc
    24121 ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg cccttacggc
    24181 ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc agaagttaaa
    24241 gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa
    24301 agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat ggatggtcgc
    24361 acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca aatctggcgc
    24421 ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg ctacagcgaa
    24481 aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc
    24541 gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa tggatagagg
    24601 tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc ttgcggacgt
    24661 ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta accggtatag
    24721 ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata cgctggggat
    24781 tcgcaaaggc gacaaggttg cactacatct cgacaactgc ccggaattta tcttttgctg
    24841 gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc tgttacgcga
    24901 ggaaagcgcg tggatcctgc aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt
    24961 ctatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc acatttgcct
    25021 gacagatgtg gcacttcccg ctgatgatgg cgtgagttcg tttactcaac tgaaaaatca
    25081 acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg cggaaattct
    25141 cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt acaacctgcg
    25201 cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg tctacctgac
    25261 ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg cgttttctgc
    25321 cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg gacaggtgca
    25381 gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt tgatggtgca
    25441 gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt atctcaactt
    25501 gtcggagcag gaaaaagatg cgttttgtga acgcttcggc gttcgcttgc tgacgtctta
    25561 tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctggcgata aacgacgctg
    25621 gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg atcacaatcg
    25681 cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat
    25741 cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag ccgacggctg
    25801 gctacatacc ggcgataccg gatactgcga cgaagagggc tttttttatt tcgtcgatcg
    25861 ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc tggaaaatat
    25921 catcgccacc catccaaaaa ttcaggatat cgtggttgtg ggtattaaag attcgattcg
    25981 cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga
    26041 atttttccgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat
    26101 cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat
    26161 gaaacagcag ggaactaccc tgccagctaa taatcacgct ataaaacaat atgcattttt
    26221 tgcagggatg ttgtcgtccc tgaaaaagca aaaatggaga aaaggaatga gtgaatcatt
    26281 acatctgacc cgcaatggat caattctgga aattaccctt gatcgtccaa aagcgaatgc
    26341 tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc
    26401 gcaattacgt gtcgccatta ttaccggtgc cggagagaag ttcttttccg cgggctggga
    26461 tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gtggatttgc
    26521 gggattaacc gaaattttca atctcgacaa accggttatc gcagctgtga acggctatgc
    26581 ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc
    26641 cagcttcgcc ctgccggaag ccaagctggg catcgttcct gacagcggcg gcgtgctgcg
    26701 tctaccgaag atcctgccgc ctgccatcgt caatgaaatg gtgatgaccg gcagacgaat
    26761 gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc aggcggaact
    26821 gatggataac gcccgcgaac tggcgcagca gctggttaac agcgccccgc tggcgattgc
    26881 ggcgctgaaa gagatcttcc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta
    26941 tattcgcagc ggcgtgttga aacactatcc gtcggttctg cattcggagg atgccattga
    27001 agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt aacaaccgtg
    27061 agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt tgtccatccc
    27121 agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc
    27181 tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat tcaggatggc
    27241 tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg ccatatcggg
    27301 cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc
    27361 gtgattatgg atggcgcggt cattggcgaa gagagcattg ttgccgccat gagctttgtc
    27421 aaagcaggct ttcacggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta
    27481 cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat
    27541 cttgtcgggc gctgtcatgc atcgttacat gaaacacagc cactgaggca aatggaggaa
    27601 aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc ttgccggatg
    27661 cgacctggtt gctggataag atgctacaac atcgcatctg gcaagcagga cgcccggtat
    27721 caacgagata aaattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc
    27781 ggttaagttc cgtgagcgga acggcaggaa tacgcggcgt ctttcttacg ccgactaatt
    27841 tatcccggct gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttta
    27901 ccagtcgctg gcactggcat ccccgtcctt ccagcttatt agggatcatc ttaacttcgc
    27961 agcttatttc tgcgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt
    28021 caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc
    28081 attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata cgaactccag
    28141 tttcacaaat taaaagtctg gctatgactc gttttgaagc aattaaacaa ggccatatta
    28201 aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg
    28261 cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa aaaaaacggt
    28321 cctcatcaga gaaccgtatt acttattcga tcaaattagc ggatgaaaaa tatccgccat
    28381 gacacgctat tatttgatct gcgcgtgcat ttcctgcacc gaaattactt tttcggtcgc
    28441 gtcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    28501 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcgc gacggcctga
    28561 agtggtgttg atgatgtagg tatattcgcc attcttgatg cggtcctgaa tgtgcggacg
    28621 gccttcatgc accttgttca ccagacgcgg attaatgccc gcttcgccca gcacaatcgc
    28681 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    28741 cacgcgttct ttatcgcctt cgcgcacgga aagcaacgca cgaccgtgtt tcttcatggt
    28801 ggagttgctg cccagctgcg ctttggcaaa cgcttcagcg aaggtgcggc ccacgcccat
    28861 cacttccccg gtggagcgca tttctggccc taacagcggg tcaacacccg ggaatttatt
    28921 gaacggcagc accacttctt tcaccgagta gtacggcggg ataacttctt tggttacgcc
    28981 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    29041 ggtggctttg gagacgaacg gaacggtacg cgccgcacgc gggttaactt caatcaggta
    29101 gacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    29161 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtaggc
    29221 tggcagagaa catgcggagt caccggagtg cacgcccgcc tgctcgatgt gctccatgat
    29281 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctaccgcgtc
    29341 atcaaggaag tgatccagca acactggcgc atcgttagac acgctgaccg ccgtctggaa
    29401 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    29461 agacggacgt accaccagcg ggtagccaat ctctttcgcc ttctctaccg ccatttcaat
    29521 agcggtaacg gtggcgttcg ccggttgttt cagtttcaga cgctcaaccg catgctggaa
    29581 gcgttcacgg tcttctgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    29641 agcagcttcc agcgcgcgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    29701 gcctttcggc ttctcaatac gcacgatttc tagcacatct tccagagtta ccggctcgaa
    29761 gtagaggcga tcggaagtgt cgtagtcggt ggagacggtt tccgggttac agttaaccat
    29821 aatggtttcg taaccgtctt cgcgcagcgc cagcgaggcg tgtacacagc agtagtcgaa
    29881 ttcgataccc tgaccgatac ggttcgggcc gccgccgaga accatgattt tttcacggtc
    29941 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    30001 ggcgaactct gccgcacagg tatccacgcg cttataaacc gggtgcaggt catactggtc
    30061 gcgcagcttg cggatttccg cttcgcgcac gcccgccagt tttgccagac gcgcatcggc
    30121 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    30181 cgctactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    30241 attggtcagg ttgaacacgc cgtccacaga caggcccgca cggaaggcat cggcgatgta
    30301 ccagatacgc tctgcgccag catctttcag ttcgcgacgg attttggtta acgcttccgg
    30361 gtcatcgagg ctcactttcg ggtcgaatcc agtcgcaccg acttccaggc cgcgcagcgc
    30421 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    30481 ctgagtggtc agacggtcgt tagcaccagc gaatttttcg aagttgaagc gaggaatttt
    30541 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    30601 gttcatcagt tcgtcgaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    30661 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    30721 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    30781 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    30841 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtatggatgc ccatcgcatc
    30901 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    30961 ctcgtactct ttccagccga tcagcgactc atcaatcagc agctctttgg ttggggagag
    31021 atccagaccg cgggcgcaaa tttcttcaaa ctcttcgcgg ttataagcga taccgccgcc
    31081 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    31141 cgccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    31201 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcggtcgc
    31261 accaatcata gtcacgccga actcttccaa cacgccctga cgctccagct ccagcgcgca
    31321 gttcagcgcc gtctgaccgc ccatcgttgg cagcaccgcg tccgggcgct ctttttcaat
    31381 aatcttgcgt actacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    31441 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    31501 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    31561 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    31621 actcctgatt acttagcggt tttacggtac tgctcaatta actcgataaa gtggtcgaac
    31681 aacggcgctg cgtcgtgtgg cccagggctg gcttccgggt gaccctggaa gctgaacgcc
    31741 ggtttatcag tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    31801 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    31861 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    31921 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag gcagatgccg
    31981 aataccggaa tatcggtttc gaggaatttc tggatggcgg taatggcgta atcgcacggg
    32041 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    32101 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    32161 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    32221 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    32281 tctgcggtgg tcacttcttt tgccagatcc atgccattca gacctgggaa cgcgcgggct
    32341 ttttctaacg ccaacgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    32401 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    32461 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    32521 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    32581 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    32641 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    32701 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    32761 agcgctgact taatcaaaac accctccaga gaatattcac tcactttatt tgcatattaa
    32821 ttcacaatga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    32881 ggcattctgg agatatgggc gctaaaagtc aacttaatgg cgggattttt tatattattt
    32941 tgtggctcat gcatgtttta caggcattaa gcggcaaaaa gatcatataa cctgttctag
    33001 aaaacgcttg cgcagcaaga agattgcatg aaataaaagc gagtagggaa aagtggacca
    33061 aatggtcaaa atttacatta aaacaacaaa aaccgaagac aaaacatgta atttttggca
    33121 cttatagaca atttaaaaac ataacactaa aaataaaagg gccaaaaatc aaagcccttt
    33181 ataatcaata aattaaatgt gttattcttg caccataaca aatattttgt ggttacaaac
    33241 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacta ctcaaccaca
    33301 aagccgatct taccgcgccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    33361 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    33421 gcacggtggc aaaaccaatg gtgccaggca cacgttcacc ggtgtggcct tcacgactgt
    33481 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    33541 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    33601 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    33661 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaatcgcct
    33721 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctggcgac
    33781 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    33841 catcttttac cgcatcgagg ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    33901 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    33961 gcacgccttc taatgccagc gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    34021 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    34081 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    34141 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    34201 tgccgataac atgtgacagg ttacgacgcc agagccagca cctcagccac ccactgctgg
    34261 aagcctttca catccagatc caatgccacc tttacattgg ctggtttgcc caggcaaccg
    34321 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    34381 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    34441 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagtgc atgaagcatt
    34501 tttccggtgc gattcaactc cggcaaagta gcgagatagt caagggttaa tattgcctga
    34561 ttggtgacat ccagaccgca catgacgatt tcgattccgc tacggaagac acaggcagcc
    34621 gcttctggat cggcagcgat attaaactcg gcgtttggcg tacagttgcc gcgtccggca
    34681 gaaccaccca tgatcaccag acggcgaata tacggcttgc attccgggca ttgtgaaagt
    34741 aacagcgcaa tattggttaa cgggccaatg gccaccaggg taacaggctc tggtgcacgc
    34801 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gcggctttcg gttgtgctca
    34861 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgccgcatc acgcggtgcg
    34921 cgtaccagcg gcacagcggc cccttgggcg agcggaatct ccgcattcca gaaatgcagc
    34981 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    35041 agttgcaggt cgagttcggg tgcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    35101 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt ctccataaaa aatgccggta
    35161 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    35221 cgacacgcag ctctttcggc acttcgaaaa caatgttttc ttcacgtcct tccagcgtaa
    35281 tggcttcacc accgcccagc tgttgcaaac gtgccaccac attctgcacc agaatatccg
    35341 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    35401 tatctttcgc atcatcaatc aaaaacgcgc ttttgcccat acgctgggcc agctccgcca
    35461 gacggttgga gttagaggag tttttcgaac cgaccaccag cacaacttcc gcctgttcgg
    35521 ccagggcacg taccgcttcc tgtcggttag tcgtggcgta gcagatgtca tctttgcgcg
    35581 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    35641 ccgacagcgt ggtctgggtc ataaaggaga gtttctcttc gtttttgacc gtcagtttcc
    35701 acacatcgtc tggtgattca accagataca ttcccccttc cgggttgctg tactgaccca
    35761 tcgtgccttc cacttccggg tgcccggcgt gaccgatgag aatagattct tcgccacggc
    35821 gactagcgcg ggcgacttcc atatgcactt tggtcaccag cggacaggtc gcgtcaaata
    35881 ccgtcaaatc gcgacttttt gcttcgttac gtaccgcctg agaaacaccg tgtgcggaga
    35941 aaatcaggat cgcgccgtcc ggtacttcgc taatctgctc aataaagata gccccacgct
    36001 cacgcaagct atcgaccaca tagcggttat gtaccacttc gtgacggaca tatatcggtg
    36061 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    36121 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    36181 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    36241 atggagtcgc cgttaatttc gcggatcacg ccaggcatct cactgccatc cattgcggta
    36301 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    36361 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccaa cgagaaggtg
    36421 gttttatcgc ccactttcag ccccaacagg tgttgctcca gcccttcaga aagagaagca
    36481 tcacccaggc ggaacagcgc cggtttaccg ttgttgcggg tagactcggc ggtggtgcca
    36541 tcctcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    36601 atgcaggttt gctcttatat tttgaggatt tttgccggat gcggcctacg atttcgcagc
    36661 aatctgtagg ccttatccgg cagggtttat tattgttttt tcgctttaga aggcaaaaaa
    36721 ccttccagca caatcagtgc cgcaccgaca caaatggcag tatcggcaag gttgaaggtg
    36781 gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga caacgaagcc gtgccacagg
    36841 cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg cataagcgat attgtttagc
    36901 ttctgcgtgg ccttcgagcg atacatcatc actgccagga tcacgctaat accaatcgca
    36961 ataccggcaa agaaccaacg ctgccagccg ccgctatcgg caaggaaact aaacgccgcg
    37021 ccatagttac gcgcataatg cagattaagc gacgggaaca gcgggaccgt atcccccaga
    37081 gcaaagttct ggaggatcag gtatttgctg cccagatcga taatcagcac gactaccacc
    37141 agccacagcc agcgtagccc tgttgaacag atcgattgac tcatcaggca aacttacgtt
    37201 tttcaccgtc accggcgacg ttgctgacac agcggccgca gatttctgcg tgttccgcca
    37261 ccttgccgac atcctgggtg tagtgccagc agcgtgggca cttctcacct tcggctttac
    37321 tcaacgcgac tttcagccct ttgagtactt cgctctgctg agcatcagca ggtgcgtcgt
    37381 tatagtctgc aacggtagcg ccggaggtca acaggacaaa tcgtaattca tcgcccagcg
    37441 cggtcagttt cgccgacagt tccggttctg catacaaggt tactgccgct tccagcgagc
    37501 cacccacttt cttgtcggca cgcgcttgct caatgacttt gttcacttcg ccacgcactt
    37561 tcaacagctc gtcccagaac gcatcgttca tcgcttcact gtctgccaga ccaaacaggc
    37621 cttcgtacca ctcaccggta aagacgtatt tttcacgttc gcccggcagg tagccccaca
    37681 cttcatcagc ggtgaaggag aggattggtg ccatccagcg caccagcgct tctgcgatgt
    37741 gatacagcgc agtctggcag ctacgacgcg ccacactgtc cgctttggcg gtgtactgac
    37801 ggtctttgat gatgtcgagg tagaaggaac ccatctcaac ggagcagaag cgcatcagac
    37861 gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt gaggatgtct tcctgtgccg
    37921 ctttcgcaca acctacggcc cagcgatcca gtaccaccat ctcttccggt ttcaccatat
    37981 cttttgccgg atcaaaaccg ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac
    38041 gacgatagct gtcggcagca cgtttcagga tctcatcaga aacggccatt tcaccggtgt
    38101 agtcagttga tgccacccac agacgcagaa tatccgcgcc cagtttgttc atcacatcct
    38161 gcggcgaaac ggtgttgccg atggatttag acatcttgcg accctgacca tccacggtaa
    38221 agccgtgggt cagtacctga cgatacggcg ctttgccttt catcgcggtg gagatcatca
    38281 gggaagacat gaaccaacca cggtgctggt cagaaccttc cagatacatg tccgctgcgt
    38341 gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg ggtagatccg gagtcaaacc
    38401 atacatccaa tgtgtccggc actttcacgt actgatcagc ttcatcgccg aggatctctt
    38461 tcgcatcaag atcccaccac gcctggatgc cgtcaacttc aacgcgtttt gccacttctt
    38521 ccattagttc gagagtacgc ggatgcagct cttccgtgtc tttgtgcacg aacagtgaca
    38581 tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc aggacggtta gcaaccatcg
    38641 actcgatacg cgcctggccc cagtccggga tccactgcac gcctttgatc tctttcagtg
    38701 actgcgcacg cagacctttc tgatccatgc tgacgaacca ctgcggcgtc gcgcggaaga
    38761 tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg ctgcattttc tcaacgtgca
    38821 gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc gttcgctttg aagacgttca
    38881 cgccatccag cgtcggataa gtgcccggca gataagtgcc gtccgggcca accgggttag
    38941 cggtttccag gccgtatttc tgaccgatca catagtcgtc cgggccgtgg ccaggcgcgg
    39001 tgtgaacggc accggtaccg gcatccaggg taacgtgatc gccgaggatt gccggaacgt
    39061 cgaagcccat aaacggatgg gtaaagcgca gcaactcaag ctccgcacct tttaccgtgc
    39121 cgagaatggt gtaatcggtc acgccgatac gctgcattac gctttcaacc agatctttcg
    39181 ccagaatcac ggcctgaccg tcgatctgca ccagcgcata gtcgaaatca ggtgcaatag
    39241 agattgcgcg gttcgcaggc agagtccacg gcgtagtggt ccagatcacc agcgagattg
    39301 ggccgttaac gttgcttacg gcaaattttg ctttcagtgc atcctgatcg accgcctgaa
    39361 aagcaacgtc gatggacgga gaagttttgt cgtaatactc aacttccgct tccgccagcg
    39421 cagaacggca gtcaacgcac cagtgaactg gcttcgcgcc tttgtgcagg tgaccgttgc
    39481 cgatgatttt gcccagcgcg cggatgatgt tggcttcagt tttgaagtcc atggtcaggt
    39541 acgggtgcga ccagtcgccc agcacgccca gacggataaa gtctttgcgt tgaccgtcaa
    39601 cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc ggcggcggtg aatttctcac
    39661 ccggcttacc gtattcttgc tctactttca gctcgatcgg cagaccgtgg cagtcccagc
    39721 caggcacata cggcgagtca taaccggaaa gccctttgga cttcacgata atgtctttca
    39781 gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt cgcataagga gggccatcat
    39841 gcagaatgaa ggtttttttg ccttttttag ccgcacggat gatgccgtac agatcatcat
    39901 cagtccaacg cgccagcatt cccggttcgc gcttggcgag atcgccacgc atcgggaacc
    39961 ctgtttccgg caaattcagg gttgatttat agtcactcat cagattctcg gttccgtgtt
    40021 tcggtttgat tacataacag gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg
    40081 ttaattcatc acgcgcaatc tgcgctttca gttcgtccag cgacgcaaat cgctgctcat
    40141 tgcgtatttt tttacgcagc actacttgta tatggcgacc gtaaaggtcc attgcaacat
    40201 ctaacaaatg cacttccagc tgctggcgaa taccggcaac cgttgggcgt gttccaatgt
    40261 ttgccacgcc gggtaacggc ttttcaccga ggcccagcac ttctaccgca taaacccctt
    40321 tcaccgggga aacctgacgg cgtagcggta cattcgccgt cgggaaacct atagtgcgcc
    40381 ctaattcatc accgtggact acacgcccgg agatggcaaa cgggtgcccc agtaaacttt
    40441 ctgccagagc cagattgtca tccgccagcg cctgacgcac agcggtgctg ctgatacgca
    40501 cgccaccttc gcaaaaagtt tgcgtactgg tgatatcgaa gccgtattcc atgccagctt
    40561 tctgtaataa caagaaatcg ccttcacgac cagcgccaaa gcggaaatca tcacctacgg
    40621 caagaaattt tacgcgcaaa tgcttcacca gaagatcgct gatgaaattt tgcgcggtta
    40681 acgccgcgaa acgcctgtcg aaacgcacgc acagcacgta atcaacgcca cactctgcaa
    40741 ggtaacgcag tttttcccgc agccgggtca gccgtgccgg ggctttatcg gtagcaaaca
    40801 gttccagtgg ttgaggttca aaaagcatca ccatcaccgg taagttgcgc ttgcgccctt
    40861 cttcctgcaa gccctgtaac agcgcgcgat gaccgcgatg cacgccgtcg aaattaccaa
    40921 tagtcagcac acacccttct tgcggggcct ggctgagatt atgtatgccg cgtatcagct
    40981 tcatgtctgg ctcaaaacag tgaaaatcgt ccgagtatac cttgtacagc ggtcaaggtt
    41041 aaccggcgat tgagtaccga aatcagaaag aagtggcttt tcattgccat ggcgcaaatc
    41101 acgggaagaa agtgaccgcc tgctgcaatt tttatcgcgg aaaagctgta ttcacacccc
    41161 gcaagctggt agaatcctgc gccatcacta cgtaacgagt gccggcacat taacggcgct
    41221 tatttgcaca aatccattga caaaagaagg ctaaaagggc atattcctcg gcctttgaat
    41281 tgtccatata gaacacattt gggagttgga ccttggctaa tatcaaatca gctaagaagc
    41341 gcgccattca gtctgaaaag gctcgtaagc acaacgcaag ccgtcgctct atgatgcgta
    41401 ctttcatcaa gaaagtatac gcagctatcg aagctggcga caaagctgct gcacagaaag
    41461 catttaacga aatgcaaccg atcgtggacc gtcaggctgc taaaggtctg atccacaaaa
    41521 acaaagctgc acgtcataag gctaacctga ctgcacagat caacaaactg gcttaatcgc
    41581 caatttgctg aagctttgtg aaaaagcccg cgcaagcggg tttttttatg cctgctgctt
    41641 ttatgatgcg tgagagaaca cactaccggt tgccagatag tcaaaagatg ctggcttttg
    41701 cagacatgcc gttacacttt ctgaactctt cgtggcttaa gaatcgtaga accatcggct
    41761 gatacattct gaacaagaat atctactcaa tctccagact cccctacaaa gaatgtggat
    41821 attgtgatac acattgaggt attaaactta agaccactct atttttgaat taaattccta
    41881 tacactttaa ccattcataa atatctcctt attagatctt gtaaataatt aacaacaatc
    41941 aacacaggtt aaatacctat ttatattcta cttcataaaa ttaatgagct atttttgata
    42001 gtagtcacat tcactcataa attaaattaa taaaaattca tcaacttcaa ggactgataa
    42061 tgacaaatgg tatctcaact tcgccacatt gtctttataa atcaaatata gtagatgatg
    42121 taataataaa taaaacaaga caaaatgagt tagtaaaagt attttgcgag tataaaacag
    42181 agttcttaat tttgtttgat gactttttta gaagccataa cttgcctaaa ccatctccag
    42241 tccttcatca tttttttcaa tatactcatc tgagagatgc atatttttat agatgtaaac
    42301 tcatagaaca tacagttcaa ttttattttt ttaaacacaa aggtatcaca ttacttagat
    42361 tggatgtttt tgatgacaga acaagcgaat gtctttctga agagattaag atataccaag
    42421 aatgccatga aaaattcgtt aattttttaa aggcaaactt caaccaagaa atatacccac
    42481 agctatatac tcctgaaata ttttatgagg cctgcagaaa tctccagtca ttttatgatc
    42541 atcaggaaac gtcacaaaac gcaaaataca gtgcaataga caagaaaaaa agtcatctta
    42601 accaggaaat caaaaagctt attaaaaaaa atattttccc tgagctatac aatgagcaat
    42661 gcaataaaat cccctcctca tcgatagatg ataaccaggg aataatgtgg cagaacttta
    42721 aaactagcaa tattgcatat tcacaactat gtaaaaaatt atctttattt aaaagcatac
    42781 ccagtcgatt aataaaaaaa tcagcttact gctcaactga aaacacaatc accaataaat
    42841 ttgatgttgt cttttcttac tgcggaaata atgtaaagga atttatactt ttattaccct
    42901 ataataaatc tctggagatg catgaactta acgagcaaaa catccagtat ttgactgccc
    42961 ttaatattaa tatacataaa atgttgctat caaatataac aatagaaaag agcgaccttt
    43021 catatgggta ttattttggt tgtgtattat ccaatatatt atgttttgaa agcgacttat
    43081 caaacaccat cttttcaaat ggagaaatta ataatctctt cattaaaaaa agcaacatat
    43141 ttggagcatc attcactaat accagaataa aaaatttgct ctgtgaagat attatgcctg
    43201 gtcgatggac cactcaactt gtcaataagc acttaggtta cagatacaca ggagtattca
    43261 aaaccctggc aagtattgat gacaaaccgt cccgtttcga aattttgata ccattggtac
    43321 aaactttagt tcgcgacaat gtaaagctaa ataatgatgt gtataaagaa cttaataagt
    43381 ttatgcacga ttacgacaaa acatcatcag aaatgagaaa ataccttaaa agcatcaacg
    43441 agtgtatgtt ccttatgaaa aacatagctc atcaaaatta aaaaatctca aaaaagaagt
    43501 aatttcagaa tgaaattaac tgtgatgatt attaattcac tacagaggaa taaaaaataa
    43561 tctgaagcat actctgaaac atcctactct ctcactctaa taatggcacc ttgctcttct
    43621 tataaagaag ggaataatta agaatattaa actttcatca acttcacatc aagaaattaa
    43681 aaattttaaa cattaatcta aataataaca acctgatcac ataatcgggt acgattttat
    43741 ccgataacta taatgctata actctgggaa atggttggaa tacaccgctg ggtgccatct
    43801 ctttttacgc tgcccgatcg agtagtaagt taaataatga taccagacac gaaggtacga
    43861 gctatcaggt cgcctataat aaatatctag tacaaacagc cactcgattt agcgtggccg
    43921 cctggcgtta tgcttcgcag gattacagga cattcagcga ccatctttac gaaaatgata
    43981 aacactatca tcagagtgat tatgatgatt tttatgatat tggcagaaaa aatagccttt
    44041 ctgctaatat tatgcaacct ttatccaata atctgggaaa tgtatcatta agtgcgcttt
    44101 ggcggaatta ctgggggcga agtggaaatg ctaaagatta ccaattcagt tactccaata
    44161 actggcaaca cattagttat actttctctg ccagccaatc ttatgatgaa aataataaag
    44221 aagaggagcg ttttaatctg tttatctcca ttcctttcta ctggggggat gatattgcca
    44281 aaacacgtca ccaaattaac ttatcgaatt cgacctcatt ttccaaagat ggctattcct
    44341 ccaacaatac tggaattact ggcatagccg gtgaacatga tcagttaaat tatggtatat
    44401 atgttaatca gcaacaacaa aataatgata cctcgcttgg tacgaattta agctggagaa
    44461 ctcccatcgc cataatagat ggcagctata gtcattctaa aaacgcctgg caaagtggtg
    44521 gaagtattag tagtggatta gttgtctggc ccggtggtat taatatcact aaccagttat
    44581 ccgatacatt tgcaattctg gatgcgcctg ggttagaagg tgcacatatt aatggacaaa
    44641 aatacaatcg aacaaacagc aaaggccagg ttgtttacga cctgataata cctcatcgtg
    44701 aaaaccatct ggtacttgat atagcaaaca gtgaaagtga aacagaattg cagggcaatc
    44761 gtcaaattat tgcaccttac cgtggagcag tttcttatgt gcagtttaca actgaccaac
    44821 gtaagccctg gtatatacag gcactgcgtc ccgatggttc gccattaacc tttggctatg
    44881 acgtactgga tctccaggaa aataatattg gagtcgttgg ccagggtagt cgccttttta
    44941 ttcgcgtaga tgaaattccc actggaataa aagttgctct caatgatgaa cagaacttat
    45001 tctgtactat tacttttcaa cacgttatcg atgaaaacaa aacatatata tgccagtaaa
    45061 aggagtttac tatgaagtgg cttttattaa tcaccttgag tctatattct tttattgtgc
    45121 aaagtgctcc ctgtgcattg accaatgtcg gtgaacaacg tggaacatat atactcaagc
    45181 cactgagtat gaaaggtaat ctaaccgccg aaaaattgaa aacaccggaa atatcattga
    45241 gtgtacagac ttgcaagaga caaccaacgc cattaaagtt cattttttaa cacagaacgc
    45301 tttaaggttt aaagtcggaa gtcgtcatta tattattaat ttcccctttg aatctgacgg
    45361 taaaaaaata gagcttccag ggaagtcatc ttataattta gaagaaatgc tatctgcgat
    45421 aaatttcacc attccatatt ctatcgcatc tgtatcagat agttctaaca ccgcagacat
    45481 ttttcctgga actccttttc ctttgttgtt cgaaattgaa gtgagttcat gtacagggaa
    45541 taatactaat tgtaatagcg tccgctttaa ttataattta aatactattt tacaggtaaa
    45601 tttaatgacc tgtggttttg aagatcaatc catcgacact ggacgatttg tactgtctcg
    45661 tatcaataat gaaaattatc agtttcatca tataagattt gactgcatcc agggcgagca
    45721 aggtgaatta gtttttgaac ccagtgatgt tgaatattac tttgaaccag tgacgataca
    45781 ggagtcagga accagcatat taaaaaatga gttagaaaac agtgaggacg gtgcgggtca
    45841 aataggcttt caactctcca atgatggtac gaatgaaatc cagtatggta aaagcaatta
    45901 ctactctttt cagcatcccc atgaaggaag taatcagatt cctctattta ttcgcccgcg
    45961 tacatatgga aataatgtct caagcgggca aataatgagc cgagtcaaaa tcgtggttat
    46021 gtacaactaa tctatctccc ccaccttaac tttgggggga ggtgccgatt aacgcgccgc
    46081 tggactaaaa agcgcagaat aatccgtatt gcagattcgc tgtaccgccg ggtgctgaat
    46141 catccgctca gcaaaaatag catggtactc ttccatcaca ttctcgacgc gaccaatttc
    46201 tacgacagtt ttatcggcat aaaagtcata tgcataaagc gttggggcaa cgaagattgc
    46261 attgtgcatc gcaccaaaag ctttcatcaa cgcggcatca tcaaactcgc cgaggatttc
    46321 tacgtttaat ccctgggagt taaaccagtt aagcaatttg cgccctaaca ttgaacgtcg
    46381 cccaggaatc aaaagtcgcc gttcttccag acaagccggg aacggttttt ctggtggtgg
    46441 atttgtacac cagaaactca cgccacattc gccaattctc acggagaaca ggccttcctg
    46501 ctgcgtagag tctatcggac agtcagaaat gatcatatcc agtttatgct gacttaattg
    46561 ctccagcagc atttcgtggg tggattcgaa gcagcgaaga tgaatgggct cgccttctac
    46621 cactgcggcg ttaagtacgc tactgaccag gcgtttggaa agtgcatcag ccacaccaac
    46681 gtcaaacaat aaattggatt ctttgcgata gttcacaata tccagcattt cctggcttaa
    46741 ggtgaacatt ttatcggcat agcgatagac cagttctccc agctcgctgg gttcgagacc
    46801 acgtcccttg cgtttaaata atttgccttg caggcgctct tccagcgctc gaatctgtcc
    46861 ggtaatggtt tgtggagtta aataaagcgc ctccgctgcg ccaaccacgg aaccttcttt
    46921 atagacatgc cagaagtaat acaagtggtt gtaattgata tgagacatgc tcatttctct
    46981 ccctgataac aatgaaaagg gagccgttta tggctccccg gtaaaccgtc ctgtcaaact
    47041 gatggacgca aacgaacgcg taaccagctg tatccaatta ccgccgaaga gatagaaccg
    47101 accaggatac cgagtttcgc ccagttaatc agttctggat ctacgctacc aaaggccagg
    47161 ctggcaataa agatagacat agtaaaaccg ataccgcaca ggatccccac cgccataatt
    47221 tgctgataag tcgttccctc aggcagatgc gccagtttca aacgcagcgc caaccagcag
    47281 aacagactaa tccccagcgg tttgccaatc agcaagccag cgatgatccc caatggcaga
    47341 atggaggtca agccatccag cgtgacacct tgcagtgaaa cgccagcatt agcaaatgca
    47401 aacagcggca aaatcagata cgccacccac gggtgtaaca catgctccag tcgcttcgct
    47461 ggagaacgcc catgcttctc tttcaaagga ataaagaagc cgacaattac ccccgccagg
    47521 gttgcgtgaa cccccgattt caacaccgca gtccacaaca ctacgccaac cagaatatag
    47581 acgcccgtgc ggcgtacacc acacagattc aataccgcga gtaccgcaat tgctacagcc
    47641 gcgacgccaa gagaggccat cgataagtca ttagtgtaga acaatgcgat gataatgatg
    47701 gccccaagat cgtcgataat agccagagcc atcaaaaaga tcttcagcgc taacggaaca
    47761 cgacttccca acagcgccag cacaccaagt gcaaaggcaa tgtcagtagc cgccgggatc
    47821 gcccaccctt cgcgggtaat cggatcggca tagttaaagg ccagatagag taatgccggc
    47881 acaatcatcc caccaatagc ggcgataact ggaaatgcgg cctggcgtaa gctggctagc
    47941 gatccttgca tcagttcacg tttaacttcc agaccgacta acaggaaaaa taccgccatc
    48001 agcgcgtcat ttatccataa cagcatgttt ttgttgattt cgagtgaacc aacccggagc
    48061 tgaaccggcg tctccagaaa gtcgtgatac catccactgg ttgcgccgct attggccata
    48121 atcatcgcca ggatagcggc aatgataaga ataatgcctc ccgaggcatc actgctaaag
    48181 aatcgatgca gatgtttcac tttttatttc tctttcaggt gaatagatcg tttttactat
    48241 tttaccccgc acaaatcatc gttaaaatca gattatagtg taggaataac tcgtttttta
    48301 cgatccattg aagaatttgc gccatcagcg gtgagatgag tttttgacgg ctgtaagcgc
    48361 tcacgagtga ccctaagccc agataatcat agcttaaggt gaggatttct gagagattaa
    48421 ttaggaaaag taataattaa tttgttgtca gtcggcgatg tttgttaggc caatatttca
    48481 aaaataatcc agcttgtcgt caataattgc gatgaagaga taaattaaag ttaaataatt
    48541 aaatcattaa aaaattacgc cataaaaatg cgaggcgtct tcaggattag aatcgataaa
    48601 acagaatggg ttaaagagag cgatataata gcggcgggtg cttgaggctg tctgtctcag
    48661 gcattagctg aacggcagat agagaaaagc cccgagtgat attttaccat caacccgagg
    48721 ccccctatat gctgaacaca tgtagagtgc ctcttactga ccgtaaggtc aaggagaaga
    48781 gagcaatgaa gcagcataag gcgatgattg tcgccctgat cgtcatctgt atcaccgccg
    48841 tagtggcggc gctggtaacg agaaaagacc tctgtgaggt tcacatccga actggccaga
    48901 cggaggttgc tgttttcacg gcttacgaat ccgagtaaga gcaacggcgg ggagtgatcc
    48961 ccgccacttt acaggtgctc gcatatcttc aacgcaccct atttttaccc aggcctgccc
    49021 acgggcaggc ttttggggag gttagcgggt caggtcgtca aaaaacttct tcacaccatc
    49081 aaagaagctc tttgagcgcg ggctgttgtg ctcgccggtt gggccaccga agctttcttg
    49141 cagctcttgc agcagctgtt tctgcttctc gttcaggcct accggtgttt cgacgacaac
    49201 gcggcacagt aaatcaccct gtgcgccacc gcggacagac ttgacgcctt taccgcgcat
    49261 acggaacagc ttaccagtct gggtttcgcc aggcactttc agtttgacgc gaccatcaag
    49321 ggtcggtact tcgatttcgc cacccagcgc cgccatagcg aagttgatcg ggacttcgca
    49381 atacaggttg ttgccttcac gctcgaaaat cgggtgctgt ttaacctgaa cctgaacgta
    49441 cagatcgcct gccggtgcgc catgctcgcc cgcttcacct tcgcccgcaa gacggatgcg
    49501 gtctccagtg tccacccctg ccgggatttt aacggacagc gttttgctgc gctcaacacg
    49561 accatgacca tgacatttgt tgcacggatc tttgatcagc gtaccgcgac cctgacagtg
    49621 tggacaggtc tgctgcacgg caaagaaacc ctgacgcatc tgcacctgac cagaaccatg
    49681 acaggtcgga caggtctgcg gctgtgtacc tggttttgca ccgctaccgt ggcaaacgtc
    49741 acactcttcc agagtcggaa tgcggatctc tttggtcacg ccacgtacag cttcttcgag
    49801 ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc gcacgttgac gaccacgtcc
    49861 gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg ctgaagtctg cgccgccgcc
    49921 aaaaccgccg ccgcccatac caccttgctc aaacgcagca tgaccatact gatcgtatgc
    49981 cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc ttgatctctt taaatttcgc
    50041 ctcggcctct ttgtcaccct ggttacggtc cgggtggtat ttcatggcca ggcgtttgta
    50101 ggcctttttg atttcacgct cttccgctgt tttggaaacg cctaaaatct cgtaataatc
    50161 ttgcttagcc atctttttta aattgcccct agatgaatgc acgggcggag aggaaattcc
    50221 ccttcgcccg tgtcagtata attacccgtt tatagggcga ttattttttg tctttgactt
    50281 cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt gtttgcagaa gcatcagcac
    50341 cggcagtctg ctgctgggca tgttgctgct gggcgatttc catcagtttc tgggaaacct
    50401 gtgccagttc ctgcattttc gcttcgatag cggctttgtc ttcacctttc agagcagttt
    50461 ccagtgcagt cagcgcagac tcgatagcag ttttgtcgtc agccggcagt ttgtcgcctg
    50521 cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc gccctggttg cgagtctgta
    50581 ccagctcttc aaacttacgg tcagcttcgg cgttagcttc tgcgtcgcgt accattttct
    50641 ggatttcatc ttcgttcaga ccagaagaag ccttgatggt gatcttctgc tctttaccgc
    50701 tgtttttatc tttcgcggaa acgtgcagga taccgtcagc atcgatatcg aaggtaactt
    50761 cgatctgcgg catgccgcgc ggtgccgggt tgataccatc caggttgaac tgacccagag
    50821 atttgttatc agccgcacgt ttacgttcac cctgcagcac atggatggtt accgcagact
    50881 ggttgtcttc agcggtagag aacacctggc tgtgcttggt cgggatagtg gtgtttttcg
    50941 cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc cagagacagc ggggtaacgt
    51001 ccagcagcag tacgtctttc acgtcaccag tcagaacacc accctgaaca gcagcaccga
    51061 ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc tttaccaaag aactcagcaa
    51121 ctttcttctg aaccattggc atacgagtct gaccaccaac gaggataacg tcgtcgatat
    51181 cagatacgga caggccagcg tcctgcagtg caactttcag cggctcaatg gaacggttta
    51241 ccagatcttc aaccaggctt tccagtttcg cacgagtcac tttgatgttc atgtgtttcg
    51301 gaccggtcgc atctgcagtg atgtacggca ggttaacgtc ggtctgctga gcggaagaca
    51361 gttcgatttt cgctttttct gccgcttctt tcaggcgctg cattgccagc ggatcgttgc
    51421 gcaggtcaat gccctgatct ttcttgaatt cttcaaccag gtagttgatc agacggctgt
    51481 cgaagtcttc accacccagg tgggtatcac cgttggtagc cagaacttcg aaggtttttt
    51541 cgccgtcaac ttcgtcgatt tcgataatag aaatatcgaa agtaccacca cccaggtcat
    51601 aaaccgcgat agtacggttg ccagtacctt tgtccagacc gtaagccagc gcagctgcgg
    51661 tcggttcgtt gatgatacgt tttacttcca gaccagcgat acggcctgcg tctttggttg
    51721 cctgacgctg agcatcgtta aagtatgccg gtacggtgat aacagcttca gttaccggtt
    51781 cacccaagta atcttcagcg gttttcttca tttttttcag cacttcagca gagatctgcg
    51841 gcggtgccat tttctggcct ttaacttcga cccatgcgtc gccgttatca gcagcaataa
    51901 ttttgaacgg catgatggaa acatcacgct gtacttcttc gtcctggaag cggcggccaa
    51961 tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt cactgcctga cgtttagccg
    52021 gctgaccaac cagagtttca ccatcctggg tataggcaat gatagaaggc gtggtgcgat
    52081 cgccttcggc gttctccagc acgcgaggag tggtgccatc cataatcgct acacaagagt
    52141 tggtagtacc caggtcgata ccaattattt tacccatcta aacgtctcca ctatatattc
    52201 ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg tctggtttca actgcccaat
    52261 tccatttttt tgcagactca ctcactgcgg ttgactacta aatggggtcg taacccacgt
    52321 catcaagggg gagatgcaaa aattttttgt gcttttgcgc agaaatttcg cgatatggca
    52381 ggacaagacg taaaattgac agcaatttta ttcatataaa gaatgaatcg cagccaaggc
    52441 attcatcaaa aaattgtaat aaaaagaaaa gattacgtac ctgaatcttc tctttatcag
    52501 cagtaaacta gtgggtattc atccctctac ctcttcccac taagagaatc cttatgaaat
    52561 ccgtttttac gatttccgcc agcctggcga ttagcctgat gctgtgctgc acggcgcagg
    52621 caaacgacca taaactcctc ggcgtcattg caatgccgcg taacgaaacc aacgatctgg
    52681 cgctgaaact tcctgtttgt cgcattgtga aacgcataca actctctgcc gaccatggcg
    52741 atttacagtt aagcggtgca tcggtttatt tcaaagccgc ccgtagcgcc agtcagagtc
    52801 tgaatattcc ttcagaaata aaagaagggc aaaccactga ctggatcaac attaacagtg
    52861 ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc cggtcatacg gtgaactcat
    52921 cggatatggc cacgctgaaa attatcggcg acgactaacg ccagaatata gaagccacaa
    52981 aaaatgaatg ttaattacct gaatgattca gatctggatt ttctccagca ttgcagtgag
    53041 gaacagttgg caaatttcgc ccgattgctc acccataatg aaaaaggcaa aactcgcctc
    53101 tccagcgtac tgatgcgcaa cgaactgttt aaatcgatgg aagggcatcc cgagcaacat
    53161 cgccgcaact ggcagctgat tgctggagaa ttacagcatt ttggtggcga tagtatcgcc
    53221 aacaaactgc gcggacacgg taaattgtat cgggccattt tgctcgatgt ttcaaaacga
    53281 ttgaagctga aagccgacaa agagatgtct acgtttgaga ttgagcaaca gttactggaa
    53341 caatttctgc gtaacacctg gaagaatatg gacgaggaac ataagcagga gtttctgcac
    53401 gcgatcgatg ccagggtgaa tgagctggaa gagctgctgc cgctgctgat gaaagacaaa
    53461 ttattggcaa aaggcgtgtc gcatttgctt tccagccaac tgacccgcat tttacgcacc
    53521 cacgcagcaa tgagcgtact tgggcatggt ttgctgcgcg gcgcggggct gggaggccct
    53581 gtaggtgcgg cactaaatgg ggttaaagcg gtcagcggca gcgcctatcg cgtgacgatt
    53641 ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta gcgccactca ggtctgatac
    53701 cggaaaccgg ggaaaatttt cattttcccc tcaaaagatc gtagacactg ccccactggc
    53761 tgattattat gccgcgccct gaaaacacta cagttattca gggaaattat ttcaccattc
    53821 attcgatgat gatttttgag gaattatggg caacactaag ttggctaatc cggcaccgct
    53881 gggcctgatg ggcttcggca tgaccaccat tctgcttaac ctgcacaacg tgggttattt
    53941 cgctctggac ggtattattc ttgccatggg cattttctac ggcggcatcg cgcaaatttt
    54001 tgctggtctg ctggagtaca aaaaaggcaa cactttcggt ttaaccgcat tcacctctta
    54061 cggttctttc tggctgacgc tggttgcgat tctgctgatg ccgaaactgg gtctgaccga
    54121 tgcgccaaat gcacagttcc ttggtgtcta cctgggtctg tggggcgtat ttacgctgtt
    54181 tatgttcttc ggcacgctga aaggcgcacg cgttctgcaa ttcgttttct ttagcctgac
    54241 cgtgctgttt gccctgctgg cgatcggtaa cattgccggt aacgccgcaa tcatccactt
    54301 tgccggctgg attgggctga tctgcggtgc cagcgcaatc tatctggcga tgggtgaagt
    54361 actgaacgag cagtttggtc gcaccgttct gccgattggt gaatcccact aattgttttg
    54421 agatcccccc gctcgggggg atttttttat tcgctaacgt cgcgtcttgc actcttcggt
    54481 ctgaatgctg caaccacttc cggtgccgtt tcaacgtatg gcccttccag caactgaatg
    54541 cagtacggta cgctggcaaa aataccgtgt accacaacgt tacccgcagc gtccttcaca
    54601 ccttccagcg tctctttaat agacttcggc tgaccgggta agttaaggat cagcgcctgt
    54661 ttgcgaatca cgcccacctg acgcgaaagg atcgcagttg gtacaaaatg caggctgatc
    54721 tggcgcatct gttcaccaaa gccaggcatc tcgcggtccg ctactgccag cgtcgcatcg
    54781 ggcgttacgt cacgacgcgc cgggccagtt ccgcccgtgg tgagcaccag atggcaactc
    54841 atttcatcca ctagctcaca caacgtttgt tcgatgatcg cctgctcatc ggggattaag
    54901 cgggtttcca gttcaaacgg cgtggttagc gccgatgtca gccattcttc cagcgcaggg
    54961 atgcctttat cctgataaac gccgctggat gcgcgatcag agatggaaac taagccaata
    55021 cgtaaagtat tcatcaagat ttttccgttt aaacagtctc gttaaacaga atgatacact
    55081 gcgaagggag tgacagacag gcttcagaag aggtagcgtg accgacttcc cggtcacgct
    55141 aagaatgatt acagcagatc gccgatcatt ttttccagtt tttcctggtc aatagcaaac
    55201 ttacggatac cttccgccag tttatctact gccattggat cctggttgtg ctgccacagg
    55261 aactcggact cagtgatacg cgccggacgc gctttcactt cgccggtgta agacagttta
    55321 cgttcgatag ccccttcgct ctccgccagc tctttcagca gtgccggtgc gatggtcaga
    55381 cggtcgcagc ctgccagttc cagaatttcg ccgatgttac ggaagcttgc gcccataacc
    55441 actgtttcat aaccgtgctc tttgtagtac tggtagattt cagatacaga aaccacgccc
    55501 ggatcttctg ccggagcgta ctctttctta tcactattcg ctttgtacca gtcaagaata
    55561 cggccaacaa atggcgagat caggaacacg cctgcttccg cacaagcacg agcctgagcg
    55621 aaggagaaca gcagggtcag gttacagttg atgccttctt tttccagctg ttctgcagca
    55681 cggataccct gccaggtaga agccagtttg atcagaatac gatcgttgct aataccagca
    55741 tcgttgtaga gtttgatcag gcgttttgct ttcgcaattg acgcttcggt gtcataggaa
    55801 agacgcgcat caacttcagt tgagatacgg cccggaacca gtttcaggat ttccagacca
    55861 atatttactg ccagtttgtc ggtcgcgtcc acgatctgct gcgcgcgatc gttgctctgc
    55921 tgtttcgccc aagcgacagc atcatcaatc aacttacggt attccggaat ctgcgctgcg
    55981 ttaagaatga gagaagggtt ggttgtggca tcctgcggtt gatacagctt cattgccgcg
    56041 atgtccccag tgtcggccac tacggtggtg tactgacgaa gggaggtcaa tttgtccgtc
    56101 atgatagtat ttctctttaa acagcttgtt agggggatgt aaccggtctg ccctgatgat
    56161 atcacgacgc aacgtcagcg caaccgccga caagacggtt atcgccaggc gagactgttt
    56221 cggatttctg acactgcgtt gatatcgccc gccattttta tacaaaacct catgtatgct
    56281 acgcagaagt tatcaagtac ctcgtagcgt atatacttct taaacaattg gtaacgttta
    56341 cacaggaaag tcatcgcgac cggcaataag agggatatgc atgccagatt ttttctcctt
    56401 tattaacagc gtcctttggg gatcggtaat gatttacctg ctcttcggcg caggttgttg
    56461 gttcactttt cgcaccggat ttgtgcagtt tcgctacatc cgccagtttg gcaaaagtct
    56521 taaaaatagc attcatccac agccaggcgg tttaacctca tttcagtcat tgtgtaccag
    56581 tcttgcggcg cgcgtgggta gcggcaacct ggccggcgtt gcgctggcaa ttaccgccgg
    56641 tggacctgga gccgtctttt ggatgtgggt tgccgcgttt atcggcatgg cgacctcgtt
    56701 tgccgaatgt tctctggcac aactttataa agaacgtgac gtcaatggac agtttcgtgg
    56761 cggaccggca tggtatatgg cgcgcgggct gtggatgcgt tggatgggcg ttctgttcgc
    56821 cgtctttttg ctcatcgcct atggcataat tttcagcgga gttcaggcga acgccgttgc
    56881 ccgagccctg agtttttctt ttgattttcc cccgctggtg acaggcatta ttctcgctgt
    56941 ctttactctg ctggcaatca ctcgcggtct tcatggcgtc gcccggctca tgcagggttt
    57001 tgtcccgttg atggcgataa tctgggtact gaccagcctg gtgatttgcg taatgaatat
    57061 cgggcaactt ccccacgtca tttggtctat ttttgagagt gcttttggct ggcaggaagc
    57121 ggcaggcggc gcggcgggat ataccttaag ccaggcgatt actaacggtt ttcagcgcag
    57181 tatgttttcc aatgaggcgg gaatgggttc tacaccaaac gcagcagcgg cagctgcgtc
    57241 ctggcctccg catccggcag ctcaagggat tgtccagatg attggcattt ttatcgacac
    57301 cctggtcatc tgtacggcaa gcgccatgct gatattactg gcgggtaacg gcacaaccta
    57361 catgccgctg gaaggtattc agcttatcca gaaggcgatg cgggtgctaa tgggttcctg
    57421 gggtgctgag tttgttaccc tcgtggttat tctgtttgcc ttcagctcca tcgttgccaa
    57481 ctacatttat gccgaaaaca atctcttctt tttacgcctg aacaacccta aagcgatctg
    57541 gtgtttgcgg atctgcacct tcgcaacggt catcggcggc accttgctaa gtcttccgct
    57601 gatgtggcaa ctggcagata tcataatggc ctgcatggct attaccaatt tgaccgccat
    57661 tttactgctc tcgcctgtgg ttcataccat tgccagtgat tatctacgcc agcgtaaact
    57721 cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat atcggtcgcc agctttctcc
    57781 ggacgcgtgg gatgatgttt cgcaggagta atcacaacta tcgatcaact cattctcttt
    57841 ttttgctaaa gtcggcataa atttcctgca aggactggat atgctgattc ttatttcacc
    57901 tgcgaaaacg cttgattacc aaagcccgtt gaccaccatg cgctatacgc tgccggagct
    57961 gttagacaat tcccagcagt tgatccatga agcgcggaaa ctgacgcctc cgcagattag
    58021 cacgctgatg cgcatcagcg acaaactggc gggtatcaac gccgctcgct ttcatgactg
    58081 gcagccagat ttcacgccgg agaatgcccg ccaggcgatt ctggcgttta aaggtgatgt
    58141 ctacaccggc ttgcaggccg aaaccttcag cgaagacgat ttcgattttg cccaacagca
    58201 tttgcgaatg ctttccggct tgtatggcgt actgcgcccg ctcgatttaa tgcagcctta
    58261 tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg aaagatctgt atcaattctg
    58321 gggagatatc atcaccaaca agctgaacga ggcgctcgca gcacaaggcg ataatgtggt
    58381 gattaatctg gcgtcagatg agtattttaa atcggtgaag ccgaagaaat tgaatgccga
    58441 gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa tttaagatca tcagcttcta
    58501 cgctaagaaa gcacgcggtc tgatgagtcg tttcattatt gaaaatcggc tgaccaaacc
    58561 agagcaactg actggtttta atagcgaagg ttacttcttt gatgaagctt cctccagcaa
    58621 tggtgaactg gtgtttaaac gctacgagca gcgttaagtt tgtagccaat gccggatgcg
    58681 gcgtgaacgc cttatccggc ctacaaaagc atgcagattc aatatattgc agaatt
//
LOCUS       DAFATA010000018        56205 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFATA010000018 DAFATA010000000
VERSION     DAFATA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.018"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..386)
                     /locus_tag="LGJ20_001480"
     CDS             complement(<1..386)
                     /locus_tag="LGJ20_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBK2385567.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCG"
     gene            complement(402..1136)
                     /locus_tag="LGJ20_001481"
                     /pseudo
     CDS             complement(402..1136)
                     /locus_tag="LGJ20_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            complement(1177..1464)
                     /gene="sctI"
                     /locus_tag="LGJ20_001482"
     CDS             complement(1177..1464)
                     /gene="sctI"
                     /locus_tag="LGJ20_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HBK2385568.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKEGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            complement(1484..1724)
                     /locus_tag="LGJ20_001483"
                     /pseudo
     CDS             complement(1484..1724)
                     /locus_tag="LGJ20_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1738..2920)
                     /locus_tag="LGJ20_001484"
                     /pseudo
     CDS             complement(1738..2920)
                     /locus_tag="LGJ20_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
     gene            3178..3609
                     /locus_tag="LGJ20_001485"
     CDS             3178..3609
                     /locus_tag="LGJ20_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HBK2385569.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLIHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTV"
     gene            3680..3973
                     /locus_tag="LGJ20_001486"
     CDS             3680..3973
                     /locus_tag="LGJ20_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBK2385570.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(4034..4426)
                     /locus_tag="LGJ20_001487"
     CDS             complement(4034..4426)
                     /locus_tag="LGJ20_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HBK2385571.1"
                     /translation="MKSDVSNSRLMISNPTHIAIGIYFKPHLSPIPLISVRVTNEVAL
                     GVRKYAKEIGIPIITDKKLARKNYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPV
                     QDEQLSSEDKFIEGEDTKIENKDDNLKN"
     gene            complement(4423..5154)
                     /locus_tag="LGJ20_001488"
     CDS             complement(4423..5154)
                     /locus_tag="LGJ20_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HBK2385572.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERIRKFFLSN"
     gene            complement(5163..5930)
                     /gene="sctT"
                     /locus_tag="LGJ20_001489"
     CDS             complement(5163..5930)
                     /gene="sctT"
                     /locus_tag="LGJ20_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HBK2385573.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(5932..6192)
                     /locus_tag="LGJ20_001490"
                     /pseudo
     CDS             complement(5932..6192)
                     /locus_tag="LGJ20_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
     gene            complement(6202..6867)
                     /locus_tag="LGJ20_001491"
                     /pseudo
     CDS             complement(6202..6867)
                     /locus_tag="LGJ20_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
     gene            complement(6857..7804)
                     /locus_tag="LGJ20_001492"
                     /pseudo
     CDS             complement(6857..7804)
                     /locus_tag="LGJ20_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(7804..7923)
                     /locus_tag="LGJ20_001493"
                     /pseudo
     CDS             complement(7804..7923)
                     /locus_tag="LGJ20_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(7936..9546)
                     /locus_tag="LGJ20_001494"
     CDS             complement(7936..9546)
                     /locus_tag="LGJ20_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385574.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            complement(9780..9853)
                     /locus_tag="LGJ20_001495"
     tRNA            complement(9780..9853)
                     /locus_tag="LGJ20_001495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9819..9821),aa:Gly,seq:ccc)
     gene            complement(9932..10687)
                     /locus_tag="LGJ20_001496"
     CDS             complement(9932..10687)
                     /locus_tag="LGJ20_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK2385575.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            11102..13399
                     /gene="xdhA"
                     /locus_tag="LGJ20_001497"
     CDS             11102..13399
                     /gene="xdhA"
                     /locus_tag="LGJ20_001497"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBK2385576.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQNGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVIINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            13410..14288
                     /gene="xdhB"
                     /locus_tag="LGJ20_001498"
     CDS             13410..14288
                     /gene="xdhB"
                     /locus_tag="LGJ20_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBK2385577.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            14285..14764
                     /gene="xdhC"
                     /locus_tag="LGJ20_001499"
     CDS             14285..14764
                     /gene="xdhC"
                     /locus_tag="LGJ20_001499"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBK2385578.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14804..16582)
                     /locus_tag="LGJ20_001500"
     CDS             complement(14804..16582)
                     /locus_tag="LGJ20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBK2385579.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNI"
     gene            17061..18248
                     /gene="ygeW"
                     /locus_tag="LGJ20_001501"
     CDS             17061..18248
                     /gene="ygeW"
                     /locus_tag="LGJ20_001501"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBK2385580.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMFLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            18306..19502
                     /gene="dpaL"
                     /locus_tag="LGJ20_001502"
     CDS             18306..19502
                     /gene="dpaL"
                     /locus_tag="LGJ20_001502"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBK2385581.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            19560..20771
                     /locus_tag="LGJ20_001503"
     CDS             19560..20771
                     /locus_tag="LGJ20_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBK2385582.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            20824..22209
                     /gene="hyuA"
                     /locus_tag="LGJ20_001504"
     CDS             20824..22209
                     /gene="hyuA"
                     /locus_tag="LGJ20_001504"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HBK2385583.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            22257..23189
                     /gene="arcC"
                     /locus_tag="LGJ20_001505"
     CDS             22257..23189
                     /gene="arcC"
                     /locus_tag="LGJ20_001505"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK2385584.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(23230..24855)
                     /locus_tag="LGJ20_001506"
     CDS             complement(23230..24855)
                     /locus_tag="LGJ20_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBK2385585.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWFGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(24903..25673)
                     /gene="yqeC"
                     /locus_tag="LGJ20_001507"
     CDS             complement(24903..25673)
                     /gene="yqeC"
                     /locus_tag="LGJ20_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBK2385586.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            25776..26354
                     /gene="mocA"
                     /locus_tag="LGJ20_001508"
     CDS             25776..26354
                     /gene="mocA"
                     /locus_tag="LGJ20_001508"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBK2385587.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            26676..29774
                     /gene="ygfK"
                     /locus_tag="LGJ20_001509"
     CDS             26676..29774
                     /gene="ygfK"
                     /locus_tag="LGJ20_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBK2385588.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPVGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            29777..31105
                     /gene="ssnA"
                     /locus_tag="LGJ20_001510"
     CDS             29777..31105
                     /gene="ssnA"
                     /locus_tag="LGJ20_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBK2385589.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            31156..31935
                     /locus_tag="LGJ20_001511"
     CDS             31156..31935
                     /locus_tag="LGJ20_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBK2385590.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            31932..34802
                     /locus_tag="LGJ20_001512"
     CDS             31932..34802
                     /locus_tag="LGJ20_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBK2385591.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            34967..36367
                     /gene="xanQ"
                     /locus_tag="LGJ20_001513"
     CDS             34967..36367
                     /gene="xanQ"
                     /locus_tag="LGJ20_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HBK2385592.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGIWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            36385..37701
                     /gene="guaD"
                     /locus_tag="LGJ20_001514"
     CDS             36385..37701
                     /gene="guaD"
                     /locus_tag="LGJ20_001514"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HBK2385593.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            37737..39104
                     /gene="ghxQ"
                     /locus_tag="LGJ20_001515"
                     /gene_synonym="ygfQ"
     CDS             37737..39104
                     /gene="ghxQ"
                     /locus_tag="LGJ20_001515"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HBK2385594.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(39140..39628)
                     /locus_tag="LGJ20_001516"
     CDS             complement(39140..39628)
                     /locus_tag="LGJ20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBK2385595.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(39628..41547)
                     /gene="ygfT"
                     /locus_tag="LGJ20_001517"
     CDS             complement(39628..41547)
                     /gene="ygfT"
                     /locus_tag="LGJ20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBK2385596.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNACEEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            41983..43431
                     /gene="uacT"
                     /locus_tag="LGJ20_001518"
     CDS             41983..43431
                     /gene="uacT"
                     /locus_tag="LGJ20_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBK2385597.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAY
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            43433..43558
                     /locus_tag="LGJ20_001519"
     CDS             43433..43558
                     /locus_tag="LGJ20_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385598.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVTMAIESRFKYMMLLF"
     gene            complement(43555..43626)
                     /locus_tag="LGJ20_001520"
     CDS             complement(43555..43626)
                     /locus_tag="LGJ20_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385599.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            43681..44229
                     /gene="idi"
                     /locus_tag="LGJ20_001521"
     CDS             43681..44229
                     /gene="idi"
                     /locus_tag="LGJ20_001521"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK2385600.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(44272..45789)
                     /gene="lysS"
                     /locus_tag="LGJ20_001522"
     CDS             complement(44272..45789)
                     /gene="lysS"
                     /locus_tag="LGJ20_001522"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK2385601.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(45799..46897)
                     /gene="prfB"
                     /locus_tag="LGJ20_001523"
     CDS             complement(join(45799..46821,46823..46897))
                     /gene="prfB"
                     /locus_tag="LGJ20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK2385602.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(46988..48721)
                     /gene="recJ"
                     /locus_tag="LGJ20_001524"
     CDS             complement(46988..48721)
                     /gene="recJ"
                     /locus_tag="LGJ20_001524"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK2385603.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(48727..49437)
                     /gene="dsbC"
                     /locus_tag="LGJ20_001525"
     CDS             complement(48727..49437)
                     /gene="dsbC"
                     /locus_tag="LGJ20_001525"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBK2385604.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTIVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(49462..50358)
                     /gene="xerD"
                     /locus_tag="LGJ20_001526"
     CDS             complement(49462..50358)
                     /gene="xerD"
                     /locus_tag="LGJ20_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK2385605.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            50470..50991
                     /gene="fldB"
                     /locus_tag="LGJ20_001527"
     CDS             50470..50991
                     /gene="fldB"
                     /locus_tag="LGJ20_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBK2385606.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(51031..51438)
                     /locus_tag="LGJ20_001528"
     CDS             complement(51031..51438)
                     /locus_tag="LGJ20_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBK2385607.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(51419..51685)
                     /gene="sdhE"
                     /locus_tag="LGJ20_001529"
     CDS             complement(51419..51685)
                     /gene="sdhE"
                     /locus_tag="LGJ20_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBK2385608.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            51928..52908
                     /gene="ygfZ"
                     /locus_tag="LGJ20_001530"
     CDS             51928..52908
                     /gene="ygfZ"
                     /locus_tag="LGJ20_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBK2385609.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(52985..53644)
                     /locus_tag="LGJ20_001531"
     CDS             complement(52985..53644)
                     /locus_tag="LGJ20_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK2385610.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(53808..54119)
                     /locus_tag="LGJ20_001532"
     CDS             complement(53808..54119)
                     /locus_tag="LGJ20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK2385611.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTITLETLTEKHAEQENMTLPELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            54164..55597
                     /gene="bglA"
                     /locus_tag="LGJ20_001533"
     CDS             54164..55597
                     /gene="bglA"
                     /locus_tag="LGJ20_001533"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBK2385612.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            complement(55654..>56205)
                     /locus_tag="LGJ20_001534"
     CDS             complement(55654..>56205)
                     /locus_tag="LGJ20_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2385613.1"
                     /translation="QVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAERINRVL
                     STNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASKGAIDTL
                     TTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQAEEVAQA
                     IVWLLSDKASYVTGSFIDLAGGK"
BASE COUNT        14506 a        14046 c        13116 g        14537 t
ORIGIN      
        1 aaaccacatg tgatgatatt gtgatagcta gcgatgccgg gtttgtatcg tgccttttca
       61 tttattttca ccggtatagc tgataaataa tcccttagca cagcaggaac cttataaaaa
      121 aatccgcgtt ctgcaaacct ttctcgataa aagtgataac cactgaataa acagaacaat
      181 ggaaataaat tccaattgtt tatgtagtaa ataagtgaac tattcagatt aaaatgttca
      241 actgaaagat tgtactgcag aacaatcatt tcattggtaa tagatctaag caccggattg
      301 ttaataggtg tattattcaa gcttactcgt tgaggatgaa tataactaat tggatcatat
      361 ataatctttc ttagggcaag attcataacg ctctcatatg tctacttaat tttcaacctg
      421 actaaattag ttagaatggc cctatacttc cataacagcc agcaagtcgc tacggatatt
      481 aatgcaagta agatagaaac cggcatagcc ttatcataag caaaaacagg ttcgctaatt
      541 tcatatgttg gtgcttgctc aataatgtct cttcgttttg acaatacaac agaaatattt
      601 tcatattgta cgcttgcaga gctattaaca ataaatctct tgatatcatt tatttttatt
      661 tctgggttga tatctttttc atatactgca agtacagaaa tatgaattgg taaagcagtt
      721 ttaccactat cgccattatc aacatcgtaa ctaacatgta ctctcgaaga aataatgcca
      781 tccataattt tgagagattg ctctaaccgc tgctcaatag cagaatatag ccttgctttt
      841 tccgctcgtg gagatgaaac gagtgcatct gcagggaaca tctgcgatat ttgaatatca
      901 ggcttacccg gtagattgta gatttttagc caatccaccg cagaagcaaa atccgttggt
      961 tcgacaaata ttgaaaatcc tgttttgcct taatccttct tttcagcatt aatattatgt
     1021 ctttgtaaaa cagcaaggac atcattagct tgctgttgat caagatggtt caataattcc
     1081 tgctgcttgc agccgcacaa cagcaggata aacaataata atgatatata tttcatgagc
     1141 gggtcaacgt ctcaactgca gcgacaatct ttcttgctaa tgttccacca atgaaacctc
     1201 gacattataa ttaagcacat gatcctgcaa gactaataat ttttcaggag ttaacccctc
     1261 tttatcagac cttaatgctt gtaacgcatt ttgctgccaa ttttcagttt gtacagcata
     1321 gttggcaaaa gcggagatca ttttatcatc cgggttgtca agattgagaa cccggtcatt
     1381 taaatcaatc actttcgctt gctgtatttc atgagcagtt gattgctcag ctctatcaat
     1441 aggtggcata ttgcttactg acataataaa atccttatcc agtttagcgg aagttttgta
     1501 tgattgctga atcaagatct ttatacgctt ttacaacgtt agattgcgca tttcgatata
     1561 atgtatactc agctaatgca ctttgatatt cggcaagaaa tttcgtgtcg gagggattgg
     1621 ttggctaaat cctccaacgc tttttcaact tgctggttca gatcatcaac gccctgatca
     1681 aattgattgc tgatatccat aatataacca ttccaatctg ccataagtcc tcctcaatta
     1741 ttcctgaaat gtccagttgc taccttgaga cttctcgaat cggttatctc cgtagctaaa
     1801 tgaactatta tttgtgtcct gatttttcaa attcacatta aatcttacat attgtttacc
     1861 ccattgttta taatattcgt caacatagtt attaatttta tttaattcac tgtcacttaa
     1921 cgtaccaatg atattgaaat acacattgtt agctgtttta tatttttcat aagaaagacc
     1981 tatcgctgac aacgtttcaa tagcctgact ttccaataca ttatcatcta ataccgcaat
     2041 acttatattg ctggcatatg gcatagtttg cagtaatcct ttaataagat tatcgattag
     2101 ttgctgtgtg atagcgttcc gttgcttact cactgtcaat cttagattac tggcatctaa
     2161 tagttgaagt ttaaaatatt gtaaatcagg gaaattatca gcaagccatg aaaaaattcg
     2221 tatcgattca tcatcaggat ttatcacccg tactggcttt cctggttgat ttttttccag
     2281 tgcttgtttg acccagactg cttcatccaa aatattgata ttaatataga taacatgatc
     2341 tctaccatat aaaacttcat aattcctgtt tttgtttaca agaattttat cgatatgtct
     2401 cagctgcgga tcattactag cattaacaat ataaaaagca ataaaaatga tagcaaaaag
     2461 aaaaaaaaag cattacatac ttaaacagct tactgctaaa gaaatgttgt tttgtagaaa
     2521 taatgttatt ttcaataatg cctggcaatg aatactgcca aacttcattt ttttctttta
     2581 cagcaaattt gagacctaaa acttctattt gctgattcaa tgttaacgtt ctatcttcag
     2641 gttcttgtgc tgttaattct ctgaatgtta ctaccagatc agggtcggta gtaattatta
     2701 tctcaaaact acctgtaagc tcgccataag gaacgactat tgtgttttct ggtaactcag
     2761 aaaaagcatc acttcttcct aacgaaacat cattaccaat gataacaagt agacgcccat
     2821 taagtatttc atactcgcat ccattcaatg gtccgctcaa caaacgaatg gcataagatt
     2881 ccaataagtc ttgtgataag aatttatcat tattttccat gaataccacc cgctaattcc
     2941 ataagctcta gtttagagta gtgtaacaag acggaaattt catgaccaaa ttatgatgac
     3001 acagaataaa aaaattataa tttcgagaac tattttaaat gcctctatgc ataactttat
     3061 ttacaattta aggtataaac cctattccct caatgttaag caaatataac aatcactctt
     3121 attttaaatt ttacacgtaa tatttaccct aaatgatttt agagtacagg tgggcatatg
     3181 caagtctttt ccagtgatgt ctacttcact gtaggcacta atgcattatt agcatcccaa
     3241 aaagaatatt acagcgattt agttgcgttg gttgatcttg gacattcgtt cgttgttatt
     3301 gatgagcatc aacatcgaaa tctcaaacca aatacagaac ctgtaaacat ccttttgagc
     3361 aacaatttca tcaggatcaa taagaatatt acattatctg atttaacgca tttcctggtt
     3421 tcaaatcttc atacacaaaa tgtgtattcc acacaagaac cacttatcca tgatgagata
     3481 gatatcctgc ggttatgtgt ttcttactcg ttaaaacaaa tagccattat taaaggaatt
     3541 gattataaaa caatatcata tcacaaaata agagccctaa ataaacttaa cataaaaggt
     3601 actgtttagt tgtttatagc actctgtgaa tgggataaac attattttaa actccaatct
     3661 tgcgtaagag aaagttgata tgtcattagt aggatataat agtaacatca taataaccat
     3721 cgcgctgata tcgttgctct ctgcatgcac cagagaaaat tcattaaagg agaatcaaaa
     3781 taaaagaagt ggcgaatccc aagcgatagt agatgcaaga cagttcttta gcgaacaccc
     3841 ggaatatata tcatcagcag aactggagca aaagctatat cgcgaatttg cacaggttac
     3901 tacagttcca gcttataaag agatgagtat gtatcaattg cttatcattt cacaagatcg
     3961 tttaactcat taaataagga tctacacgct ttttgcgtgt agattcttat aaatttctga
     4021 ctacgacaga tgtttaattt tttaaattat cgtctttatt ctcgattttt gtgtcttcac
     4081 cctcaatgaa tttatcttct gaagagagct gttcatcttg aacaggttgc ccagcattct
     4141 ccacatcttc aagccacagc agaagacgta atatttcatc tatattttcg aagctaacat
     4201 aatcgtagcg acgatgggta gcataatttt ttcgtgctaa ttttttatct gtgataattg
     4261 gtattccgat ttcctttgca tatttcctta cgcctaaggc cacctcatta gttactctta
     4321 cagaaatcaa tggaataggt gaaagatgtg gcttaaagta tatccctatt gcaatgtgag
     4381 tagggttgga aatcatcaaa cgactattac tgacatcaga cttcaattgc tcagaaagaa
     4441 tttcctgatg cgctctctgc gtttagactt aatttctgga tttccttctt gctccttgta
     4501 ttctctttta acttcttgtt tatccatttt catatctttc ataaataaaa aatattcagc
     4561 gataaaatca aaaattaaga caattatcat actgccgaga caatacagta tgagaaggaa
     4621 tagcatctct ccccaatctg ataataaaga tatgatatct ccatcaagag ttttaaaaag
     4681 cagtgattta tgattactcc aaaatacttt gatcgccaat gcaaaaaaaa caatataaag
     4741 aattgcttta acaaattctt ttacggtttt taagccaaat atacgcttta agccatttac
     4801 tggattcaat gaatcaaact taaatttaac agcttcagtt gctaattgca ttttactttg
     4861 aaaccaggag acaagtattg ttgtgaacat gcaaaccagc aaaaaaggaa tgttcgtttt
     4921 caaccaacca acccccatag ctttaaaata gtcccaaata tctggtttag cgttatgatc
     4981 aaggatgtat tcaagaatcg acataatgtg atgcacatca aaaacatatc cgagatataa
     5041 agtacccaca agcattatta cagagatggt taagtctcta ctttttagaa tttgcccttt
     5101 tttagaagca tcctggagtt tcttttgtgt tggcttttca gttttattgg ccatgaaaca
     5161 ctctatttct caatgtatag ttgcaattgt tctgggaaaa aggataacgg cattactcgt
     5221 tcagccagta ttggggcaaa ataaataatt aatattaaaa atgccagcgc acttttgaca
     5281 gtcagtgaaa tggcaaaagc atttagttgt gaagcataac atgccagcaa tcccaggaca
     5341 gcctcaccgc ccagcataac ggcaatcacg gggcttgcgt aaacgatagt atgagtcatt
     5401 atgttattaa tatatgagaa taaaggctct aatttaggaa aattaaaatt gcctgaaggc
     5461 cataaattat aactttgcca aagagtttcc agaataaagt tcattccacc tttagtaaga
     5521 taaaccgcag cagagaaaag gttaaataat cttgctaatt ccgaggtatc gacacctgta
     5581 gccggatcca aagtactact taatgttgca ccgcgttggt tatcgataaa gctacctata
     5641 gcaaggaaca tccaaaacgg ggatgctaat aagcatgcaa taaacagccc tatgataatt
     5701 tctctggcag cgaacaaagc aatatcgatt tcatttaagt tcaataattc atagtggtaa
     5761 tgctgaacta atgcacatga taccactatt attattgggt gacgaactac tgttggtata
     5821 ttcccactgg cgaaaaatgg taacaaatag aaagttggtg ccaatcggca aaaaccgagc
     5881 gctgtagcag ccagcagact atgcaactga tagagtatcg cctcccccat gctatcctct
     5941 cgcgaatgct aaattcagca tttcgatacc aaatgaatat aatttttcac cataccaccc
     6001 gctcattaaa aaaaagcaaa tacttacgca caataatttc acgccaaatg gcaacgtctg
     6061 ttcttgcaac tacgttaccg tttgaaaaag ccctaccaat aaaccaacaa atgtcgcaac
     6121 tgcaataggt cctgcagaca taactaaaat cagatacagc gccctattcc ccgcaaaaac
     6181 aatatcgtcc atattttttc ctcagggatt aatggatagg tcaaaatatt gtaaaatgag
     6241 cccttttgat aacattgtcc aaccgtccat cgcgacaaac aaaattaatt ttatcggtgt
     6301 agatatggtt accggactca tcatcatcat acctaacgtt aacagaacgc tggatataac
     6361 cagatccact acgacaaagg gtaaatagat ataaaaacct ataataaatg cactttttat
     6421 ctcactcaat gcatatgcgg gcagtaacga gaaaattgaa atattatcat catcgattat
     6481 ctcttcattg tcttcagaac tattcacctt ctaaatcttt tcaaaaaagc taaccaactc
     6541 aggttcagaa tattttatca aatatgattt ataaccactc atgcctgtct caacaaaatt
     6601 aacaaccgat gctacattat tgaatgaaag attctcattc tgactgttat tatatatttc
     6661 cttcccaacc ggcatcatta cgaacattga caatagcaaa gcaaccccat taagtgtcat
     6721 atttgatggc acttgctgaa gtcccagcgc gtttcggaca ataacaaaaa caatcgagaa
     6781 tttaataaaa catgtcccgg atgctattat aaacggcaat agtgtaaata atgataatat
     6841 cgctatcagc gaaatgctat ttgacattat tattccctga taaccaagaa tgaatttcta
     6901 cgcccaattt atcatccact tcaacaagtt cgccatagcc agttagcgca ccatttacac
     6961 gtatctctat atttttctca gattcaggaa gcagagatat tattcttttt gcacagagat
     7021 cgtctatttc atatagattc attattttct taccaagaac aaactcaatt tttacaggca
     7081 actcttcaaa actattaatc atctgttcgt cattttcatc ataaatctca ctctcgtttt
     7141 ttttgatatc aaaatcatct gtttcaaaat cttcctcgta ctcaaaatga tcagccattt
     7201 ttaactcctc tggataaatt aaatcacaaa tttttgtatt ataaataacc gcataagcca
     7261 aattatttga gattaataaa acatcaccaa cttcaatatc aacaagactt ctgtaacatg
     7321 ttttactaaa tccaattaca aaatgaatgg accaatataa tgattttttg tctaacactg
     7381 gagcgtttcc gtattggcag actgtgactt tatctaacca aagattcaat tcccctgttc
     7441 atattcttat tagtggttga gcagggctca atactccatc aaatacttta cctacgtgca
     7501 aacgattata ttttagttca ggatgcttta gatttaacgg ggaactggat aaactgagcc
     7561 attcccctat taacctttcc gtttctggca ttaaagccat cccagcgagc tcagatgaaa
     7621 ttaagggaaa tatttctcca agcaataccc atccagacca gctgccactc tcttctgcta
     7681 tcggtaacca tttagcacta aattcgggca ttaataatgc aacatcttcg ccattttctt
     7741 tccaattttg aaaagtggtt tcaaacgtat gagtgtttcg atttactttc ctcagaccga
     7801 acattaactt tcatccttct gatcgttttc gaatttgctt tcattttctt gctcctcata
     7861 aggctgatgt cgttgtcctt gtcgttcatc ttgctcagtc aatacccaac gttgtgctgt
     7921 aactcattat gaaaactatt taattccctc atcattgata aggttggaca tctctttcaa
     7981 catgagagtc attgaagctt tcgaccaatt tagcggaatg atagggcttg gcgcttcatg
     8041 caaagttccc gacttgtgga tatagttata gctttcggga agtgccattt caggtacact
     8101 cctgccattt gcaccaatca tattctcgcc tgttatttgg gccaatgatc tattcataaa
     8161 ttgcactgag tcattcaaat actcttcctt tcttgattct ttataaacaa ttgctgctga
     8221 ttttgcatac catgagtcaa aaaaccattc tgcttctgtt gatggaatga agctcttttc
     8281 tcttttcgca tgtgaatttt gatcggtatc ggttttaata tcattgaacc aaaaatttgc
     8341 cgactgataa ttatccttct cgtatctttt tataccatac ggacctgcta gttttttaac
     8401 tatttttaga acctgctctt ttcttctggt gtttatcttt gcaagatttg caggataaat
     8461 aacattaagt aaagccgcat cggcttctct gtaatgttta tctttttcca gataacctgg
     8521 ggattctccc cctaaatcaa gctgtaacgt tattctttca taacctttat ctatgaggtg
     8581 gttcaacctc gttgttgaaa gaggttcatc cagttcattg gctttagctt cgcgtagtaa
     8641 atcagagaca aaaacagagt ctttcttcga gagtaagttc gacaaacgtt ccagtccaga
     8701 ggtcaccagt gcaacggaag atgtgtttaa tcgggcatct tcttcccatg ctccggaatc
     8761 ttccataaca tagaaattgg ctttatccag ataagcaatc agcagcgcaa ccgattttaa
     8821 tcggtcacct ttttgccaat cttcagcgtt aattgtatca gtgtcgattg cttgtataag
     8881 gagatcaaga taaagaccta acgcatcatt ttgtttatgg ttccacagct gcggcttgcc
     8941 ttcttcctgc acatctgcca tcacggggga gttgctgtca aatcggatat gcacaacatt
     9001 catttgcccg ggaactccat ccagtcgttt ggggttgcta atgacgtcct gcatccgctt
     9061 aatctggtcg ggcgtagaca tataatccca cagggtgagc agaacctttt ttgcggctac
     9121 actatttccc tgatctgata ctaatgccat atagccccat aaactatccc tcagccagac
     9181 agcatcatag tgggtttctg ccgctgtttt gtcatcttcg aaagtagaag caagaataaa
     9241 cgttccccag ggtttctccg tcaacgttaa ctttcgtgtg ttttctgtta atgttagcaa
     9301 tccttgaatt tcgtctttgg tatagtggct ctttatttca ttttgtaatt tggctgagtg
     9361 aatcaaaaga cgataattag gatacttatc tggatttaac gataaataac ttttatctgt
     9421 tgctgccccc gtggaattaa gaatgttaaa tacagattca ctcgtcactt ctgcccctgt
     9481 cgcctggcat gtgccgcacg aaaatatacc agcgagcaga agagttaatg cagatttccc
     9541 tttcataaaa tattccatca atcgtttgtt aaaagcgtag ataaccactt atttaacaac
     9601 ttgaaaataa ggtgttttac ctgggttgtt acaaaaggat tgcattgcgt aaacgctttt
     9661 tatttacaac aaaatgggga agtattacgg cgattgcgaa aagtctgaag cgattgaatt
     9721 aagataatat agaaaatcag atacagcaaa aatggcagat tgatcaggtg aagataaatt
     9781 ggagcgggcg aagggaatcg aaccctcgta tagagcttgg gaagctctcg ttctaccatt
     9841 gaactacgcc cgcttcgaga tgcgtaaggc attataaacc ttacgctctc cttagcaagt
     9901 gccgcgctgc tgactgctga ttaattcgcc atcagcattt tggcttgctg ccctgcggcg
     9961 gcaagtaacg tagcggatca attgccgttg cacggtaacg aatctggaaa tgcaggcgaa
    10021 cagatgccgc atccgtgctc cccatagtgg cgattttttg cccagccttc acgctttgcc
    10081 cattatttac cagcatcgtg tcattatggg cgtaagccgt aatgtaatct tcactgtgtt
    10141 taatcatgat gagattaccg tagccacgca gctggttgcc cacatacacc acctttcctg
    10201 cacccgcggc gtaaataggt gtaccccgtg gagctgagat atcaatccct ttattgccgc
    10261 catctgctgt cgaatacggc atgataactt tccctgtcgt tggccataac caacaacgtt
    10321 gccctactgg cggccaggaa gatttcggta ccgctgatga cggtgtaacc gatgcggttt
    10381 tggtcgttga tttggcggtt gatttacgtg tactactgct acttttcgcc ccacctagtt
    10441 ttagtttctg accaacttca atggtgtaag ggggggaaat gccgttcagt cgcgccagtt
    10501 cttttacgct ggttcccgtg gtgcgcgaaa tacgatatag cgtatccccc cgtttcacgg
    10561 tgtaaacgga gccggaatac gttcctgtat cggatgattt gctacccgaa cagcccgcca
    10621 aaagcagtcc aaccgataac aacatcacga tacccagaga ttttttattc aggcgtcccg
    10681 cactcaaaat ctttcctcgc ttaaaaaaca agacgcctta tcatagcagc cagcctcttg
    10741 atacccaatc tcttgcttct ggaaaaacgt ggtaatagag aaagaggtta atttcatttg
    10801 gcaaagggaa gtatcatcag caaaatattc gctgtagagg tatcgaactt cctgactttt
    10861 taccctattt cccatcctga tgcgtaataa tcccaattta tcacattgat tttatcgcca
    10921 ctcatattga tacgtatcac tgcccctatt cagagtcctg tttcgtatca tatctgtgtt
    10981 tttttaacgt catcacactt cctacctccc ctaacgctta tcgtcgtttc tggcgtaaat
    11041 cttgcctgct tagactaaat ctttgccata agaaccaaat gttcaaatcg aggggatttc
    11101 tatggaagcg cgggaagcaa ccgctacggg tgaatcatgc atgcgcgtcg atgccattgc
    11161 taaggtcacc gggcgggcac gatatactga cgattatgtt atggcgggca tgtgttatgc
    11221 gaaatatgta cgtagcccta tcgcacatgg ttatgccgta agtattaatg atgaacaagc
    11281 cagaagtttg ccgggcgtac tggcgatttt tacctgggaa gatgtgcctg atattccatt
    11341 tgctactgct gggcatgcct ggacacttga cgaaaacaag cgcgataccg ccgatcgcgc
    11401 actgctaact cgccatgttc gtcatcatgg tgacgccgtt gccatcgtcg tggcccgcga
    11461 tgaactcacg gcagaaaaag cggcgcaatt ggtcagcatt gagtggcaag aattacccgt
    11521 tatcaccacg ccagaagcgg cgctggcaga agacgctgca ccaatccata acggtggcaa
    11581 tttactgaaa caaagcacga tgtcgacggg taatgtccaa caaacaatcg atgccgccga
    11641 ctaccaggta caggggcact atcagacccc cgttattcaa cattgtcaca tggaaagcgt
    11701 aacatcgctg gcgtggatgg aggatgactc gcgaattacc atcgtttcca gcacccagat
    11761 cccgcacatt gttcgccgcg tggttggtca ggcgctggat attccctggt catgcgtacg
    11821 agtcatcaaa ccgtttgtcg gtggcggttt tggtaataaa caggatgtac tggaagagcc
    11881 aatggcggca ttcctgacca gcaagcttgg cggcattccg gtgaaagttt cccttagccg
    11941 tgaagagtgt ttcctcgcaa cccgtacccg ccacgctttt accattgacg ggcaaatggg
    12001 cgtgaaccgc gacggaacat tgaaaggtta tagtctggat gttctgtcta acaccggcgc
    12061 ttatgcatct cacgggcact ccatcgcttc tgcggggggg aataaagtcg cttaccttta
    12121 tcctcgttgt gcctacgctt acagttcaaa gacctgctat accaacctcc cctcggctgg
    12181 tgcgatgcgt ggttatggcg cgccacaagt cgtatttgcc gttgagtcta tgcttgatga
    12241 cgccgcgaca gcgttaggta ttgatcctgt tgaaattcgt ttacgcaacg ccgcccgcga
    12301 aggagatgct aatccgctca cgggcaaacg tatttacagc gcagggttgc cggagtgtct
    12361 tgaaaaaggc cggaaaatct ttgaatggga aaaacgccgt gcagaatgcc agaaccagca
    12421 aggcaatttg cgccgcggcg ttggcgtcgc ctgttttagc tacacctcta acacctggcc
    12481 tgtcggcgta gaaatagcag gcgcgcgcct tctgatgaat cagaatggaa ccatcaacgt
    12541 gcaaagcggc gcgacggaaa tcggtcaggg tgccgacacc gtcttctcgc aaatggtggc
    12601 agaaaccgtg ggggttccgg tcagcgacgt tcgcgttatt tcaactcaag ataccgacgt
    12661 tacgccgttc gatcccggcg catttgcctc acgccagagc tatgttgccg cgcctgcgct
    12721 gcgcagtgcg gcactattat taaaagagaa aatcatcgct cacgccgcag tcatgctaca
    12781 tcagtcagcg atgaatctga ccctgataaa aggccatatc gtgctggttg aacgaccgga
    12841 agagccgtta atgtcgttaa aagatttggc gatggacgct ttctaccacc ctgaacgcgg
    12901 cgggcagctc tctgctgaaa gctccatcaa aaccaccact aacccaccgg cgtttggctg
    12961 tacctttgtt gatctgacgg tcgatattgc gctgtgcaaa gtcatcatca accgcatcct
    13021 caacgttcat gattcagggc atattcttaa tccactgctg gcagaaggtc aggtacacgg
    13081 cggaatggga atgggcattg gctgggcgct atttgaagag atgatcatcg atgctaaaag
    13141 cggcgtggtc cgtaacccca atctgctgga ttacaaaatg ccgaccatgc cggatctgcc
    13201 acaactggaa agcgcgttcg tcgaaatcaa tgagccgcaa tccgcatacg gacataagtc
    13261 actgggtgag ccaccaataa ttcctgttgc cgctgctatt cgtaacgcgg tgaagatggc
    13321 taccggtgtt gcaatcaata cactgccgct gacgccaaaa cggttatatg aagagttcca
    13381 tctggcagga ttgatttgag gataacatca tgtttgattt tgcttcttac catcgcgcag
    13441 caacccttgc cgatgccatc aacctgctgg ctgacaaccc gcaggccaaa ctgctcgccg
    13501 gtggcactga cgtactgatt cagctccacc atcacaatga ccgttatcgc catattgttg
    13561 atattcataa tctggcggag ctgcggggaa ttacgctggc ggaagatggc tcgctacgta
    13621 tcggctctgc aacgacattt acccagctaa tagaagatcc tataactcaa cgtcatctcc
    13681 cggcgttatg tgctgcggcc acgtccattg ctggaccgca gatccgtaac gtcgctacct
    13741 acggtggaaa tatttgcaac ggtgccacca gcgcagattc tgccacgcca acgctaattt
    13801 atgacgcgaa actggagatc cactccccgc gcggtgttcg tttcgtcccg attaatggct
    13861 ttcacaccgg gccgggcaaa gtgtctcttg agcatgacga aatcctcgtc gcctttcatt
    13921 ttccgccaca gccgaaagaa cacgcgggca gcgcgcattt taaatatgcc atgcgcgacg
    13981 caatggatat ttcaacgatt ggctgcgccg cacattgccg actggataac ggcaatttca
    14041 gcgaattacg cctggcattt ggtgttgccg cgccaacgcc gattcgctgc caacatgccg
    14101 aacagactgc acaaaatgcg ccattaaacc tgcaaacgct ggaagctatc agcgaatctg
    14161 tcctgcaaga tgtcgccccg cgttcttcat ggcgggccag taaagagttt cgtctgcatc
    14221 tcatccagac gatgaccaaa aaagtgatta gcgaagccgt cgccgcggcg gggggaaaat
    14281 tgcaatgaat cacagcgaaa caattaccat cgaatgcacc attaacggga tgccttttca
    14341 gcttcacgcc gcaccaggca cgccgctctc ggaattactc cgcgaacaag gactgctaag
    14401 tgtcaaacaa gggtgctgcg tgggtgaatg tggtgcctgt acggtgttgg tcgacggcac
    14461 agcaatagac agttgcttat accttgccgc ctgggctgaa ggaaaagaga tccgcacgct
    14521 ggaaggtgaa gcgaaaggcg gaaaactttc tcatgttcag caggcttatg cgaaatccgg
    14581 cgcagtgcag tgcgggtttt gtacgcctgg cctgattatg gctaccacgg caatgctggc
    14641 gaaaccacgc gagaagccat taaccattac ggaaattcgt cgcggactgg cgggaaatct
    14701 ttgtcgctgc acggggtatc agatgattgt aaatacagtt ctggattgcg agaaaacgaa
    14761 gtaaaaggat atccggcctg aattcaggcc ggattcactg aggttatatg tttaacaact
    14821 catatttctt aatcttgcga tagagcgtag caatgccgat gcccagttca tcagcaactt
    14881 gcttcttgct gttatgacgt gaaagcgcct cgcggatcat ttgcttttcc atctcctcca
    14941 gcgccgtgcc gcccgcatca tcgagtgaca ggtgcgcctc actgacctct gttacatcac
    15001 tttgctccgt tgtgccatta ttcagcagat ttggcggcaa tagcgtgctg tcgataactt
    15061 cacctgaagg aactacgtta accagatatt ccatcaaatt gcttaactcg cgcaggtttc
    15121 cgggccaacg atgcttacgc aatatttcga cgacatcggg agcaatgcca ggataaaccg
    15181 atcccagacg acgggtatgc agatgtaaaa agtaatgcac caatagttca atatcttcct
    15241 gacgttcacg cagcggtggc agagttatcg ggataacatt aagtcggtag aagagatctt
    15301 cgcggaattt accttcggca atgaactggg ccaaattctg attagttgca gaaatgatgc
    15361 gaatgtcgac ttgtattggg ctactggcac caatcggcag aatttcacgc gcctcaatag
    15421 cgcgcagtaa tttagcctgc aacattaatg gcatatcacc tatttcatcg agaaacagcg
    15481 tgcccgtatt cgccgcctga atcaaccctg ttttaccgtt ggcagaagcg ccagtaaatg
    15541 cgcctttaac ataaccgaac agttcgcttt ccagaagctg ctccggaatc gcggcacagt
    15601 tgatagcaat aaagggttta ttccgtcttc cgctcaactt atggattgct cgggcgacga
    15661 cttctttacc cgtgccgctt tcaccaacca ccataacgct ggatgggctg ggtgcaatac
    15721 ggctaatgag tcgttttaat tgccgcataa cacggcactc gccaaccaat tgttcaatat
    15781 gtggttcatc aggtgcattt gctacagaaa aactggtatg cgattggtga aatgccatta
    15841 aaaataattg tcggccctga atgttatgca attgaccaat gattaattca cttttatcgt
    15901 cccacgaaac aatatgctgc atatgtccat gagtaaaatt actctcaaat gttaatggtc
    15961 tgaaacggat aggtttccca ataatattat tttgtacaac accaagtgtt tttaaggcag
    16021 tctgattaac aaactgaacc cgactttcat catcaacaac taatacgccc tgatccatat
    16081 tatcgatcat ggtcgcgaat attttactga tgttatctcc tggcccctga tcctccagaa
    16141 gtttcgaaac aaaaatggtg gatatatggc gaacataatc agaaaattcg cgtaaattat
    16201 cactgatatg ctcttgttgc tcgtgggtaa cggcaatcaa acttatcacc ccaacacaac
    16261 gatcctgtaa aatgacaggc gtacccagaa atgctttttc gcggcaattt tctttactat
    16321 cgcaaccttc gcaaagggga tcgaagcgag actgtgtcac aactttttca gttttcgttt
    16381 ccaggacgtg gcggagcagg cgtgagttgc cgctcaactg gcgaccaaga aacttcccat
    16441 acgcgcccgt tccggcgacg cgacacaagt tttcatcaac gatctcaacc tcaagctgca
    16501 aaacgctggc aagcattctg gcaaaacgct gaattgtcgg ttgaatttgc atcaatactg
    16561 actgcgtagt agcaagctcc atagctttac cttccagact tacttaaaag tcgatcattg
    16621 aagacgttga tggttcacag atcatgatga tattaactca ggcgaaattg gctttgataa
    16681 aaacataaga tttttatcat tttctaatga aattatggaa gagatatcac atttctatat
    16741 caatatgaga attacggcgg tgagtttatc aaactgaaga gagatagcct gtccctttat
    16801 cttatttctg atacttagca gcaaataaat aacgcgataa aaaaagccaa acgttttcgt
    16861 attttacaaa caaccggaag ctggcatcaa tttgtgatca accccacaca ttatccgtca
    16921 aattagtctt ttgcagcccc gtggataatt ctggcacact tattgttagt cccaggtata
    16981 gctgtgaaaa caccaatcac tttggcaagt cacagtgaaa taaaccactt tgcctgtcat
    17041 tccactaccg ggactttatg atgaaaactg ttaatgagct gattaaggat atcaattcgc
    17101 tgacctctca ccttcacgag aaagattttt tgttaacgtg ggaacagacg ccagatgaac
    17161 tgaaacaagt actggacgtt gccgcagcat taaaagcact gcgtgctgaa aacatctcaa
    17221 ccaaagtctt taatagtgga ttaggtattt ccgtattccg cgacaactcc acccgtaccc
    17281 gcttctctta tgcttccgcg cttaacctgc tcggccttgc acaacaagat ctcgacgaag
    17341 gcaaatcaca aatcgctcac ggcgaaaccg tgcgtgaaac cgccaatatg atctccttct
    17401 gcgccgacgc tattggtatt cgcgacgata tgtttctggg cgcaggcaac gcctatatgc
    17461 gtgaagttgg cgctgcactt gatgacggtt acaagcaggg ggtactgcca cagcgtccgg
    17521 ctttagtgaa cctgcaatgc gatattgacc acccgactca gtcaatggct gacctcgcgt
    17581 ggttacgtga acactttggt tcactggaaa acctgaaagg taaaaaaatc gccatgacct
    17641 gggcctactc tccaagctat ggcaaaccgc tctctgtacc acaaggcatc atcggtctga
    17701 tgactcgctt cggtatggat gtcaccctgg cccatccgga aggctacgac ctgatcccgg
    17761 atgtggttga agtggcgaaa aacaatgcta aagcatctgg cggtagcttc cgtcaggtca
    17821 ccagcatgga agaagcgttc aaagatgcag acatcgttta tccgaagtca tgggcacctt
    17881 acaaagtgat ggaacagcgt accgaattgc tgcgcgctaa cgatcacgaa ggcttaaaag
    17941 cactggaaaa acagtgtctg gcacagaacg cgcaacacaa agactggcat tgtactgaag
    18001 agatgatgga actgacccgt gatggcgaag ccctgtacat gcactgcctg ccagctgata
    18061 tcagcggcgt atcctgtaaa gaaggtgaag tgactgaagg cgtattcgaa aaataccgta
    18121 tcgcaaccta caaagaagcc agctggaagc cttatatcat cgccgcgatg atcctgtccc
    18181 gtaaatacgc caaaccaggt gcactgctcg agcaactgct gaaagaagcg caagaacgcg
    18241 tgaaataacc cctcgggccg atcagctggt cggccctcta tttccagagg ccaaaaggat
    18301 aggatatgtc cgttttctca ttgaagattg atatcgccga taacaaattt ttcaacggcg
    18361 aaacatcacc gctcttttcg caaagccagg ccaaactggc gcgccagttc caccagaaaa
    18421 tagctggtta tcgcccaaca ccgctttgtg cgctggacga tctcgcaaac ctttttggtg
    18481 tgaagaaaat tcttgtcaaa gacgaatcaa aacgattcgg tctgaacgcc ttcaaaatgc
    18541 tcggcggtgc gtacgccatc gctcaattat tgtgtgaaaa atatcatctt gatatcgaaa
    18601 cgctgtcatt tgagcacctg aaaaatgcca tcggcgaaaa aatgactttc gcgaccacca
    18661 ccgacggcaa ccacgggcgc ggtgtggcgt gggcagcgca gcaactcgga cagaatgcgg
    18721 tgatttacat gccgaaaggt tctgctcagg aacgcgttga cgccattctg aacctcggtg
    18781 ccgagtgcat cgtcacagat atgaactatg acgataccgt tcgcctgacc atgcaacacg
    18841 cgcagcagca cggctgggaa gtggtacagg acacggcatg ggaaggttac accaaaatcc
    18901 caacctggat catgcaaggc tacgcaaccc tggcagatga agccgtcgag caaatgcgtg
    18961 aaatgggcgt aaccccgacg cacgttctgc tgcaagccgg tgtcggagca atggccggtg
    19021 gtgtgctggg ttatctggtc gacgtctata gcccgcaaaa tctgcacagc attattgttg
    19081 aacctgacaa agctgactgt atttatcgct ccggcgtcaa aggcgacatc gtcaacgttg
    19141 gcggtgatat ggccaccatc atggcaggcc tggcctgtgg cgaacctaac ccgctgggct
    19201 gggaaatcct acgtaactgc gccacccaat tcatctcctg ccaggacagc gttgccgcat
    19261 taggtatgcg cgtgctgggt aatccgtacg gcaacgaccc gcgcatcatc tccggtgaat
    19321 ccggcgctgt cggtttgggc gttctcgcag cggttcatta tcacccgcaa cgtcaaagcc
    19381 tgatggaaaa actggcgctg aacaaagatg ccgtggtgct ggttatcagc actgaaggcg
    19441 acaccgacgt gaagcactac cgcgaagttg tctgggaagg caaacacgct gtagcacctt
    19501 aatcacccta ttggaactgc tccctgcaaa acggggagta aaaaatctgg agaaaaataa
    19561 tggctaagaa tattccattc aaactgattc ttgaaaaagc aaaagattac caggcggaca
    19621 tgactcgctt cctgcgcgac atggttgcta ttcccagtga aagctgcgac gaaaaacgcg
    19681 tagtacatcg tattaaagaa gagatggaaa aagtcggctt cgataaagtt gaaatcgacc
    19741 cgatgggcaa cgttctcggt tatatcggcc acggcccgcg tctggtggca atggacgctc
    19801 atatcgatac cgtcggcatt ggcaacatca aaaactggga cttcgatccg tacgaaggca
    19861 tggaaactga tgagctaatc ggtggtcgcg gtacttccga ccaggaaggc ggcatggcat
    19921 ctatggttta tgccggtaaa atcattaaag acctcggtct ggaagatgaa tataccctgc
    19981 tggttaccgg tactgtgcag gaagaagact gcgacggtct gtgctggcag tacattattg
    20041 aacaatccgg cattcgcccg gaatttgtgg tcagtaccga accaaccgac tgccagatat
    20101 accgtggtca gcgcggtcgt atggaaattc gtattgatgt tcagggtgtt agctgccacg
    20161 gttctgcgcc agaacgcggt gacaacgcca ttttcaaaat gggtccgatt cttggcgaat
    20221 tacaagaact ctcccaacgt ctgggttatg acgaattcct cggcaaaggc accctcaccg
    20281 tttctgaaat cttcttcaca tccccaagcc gttgcgctgt agcagatagc tgcgccgtct
    20341 ctattgaccg ccgtctgacc tggggcgaaa cctgggaagg cgcgctggac gaaatccgcg
    20401 ccctgcctgc agtacagaaa gctaacgcgg ttgtttctat gtacaactac gaccgtccgt
    20461 cctggactgg cctggtttac ccaaccgaat gctacttccc gacctggaaa gtggaagaag
    20521 atcacttcac cgttaaagca ctggtgaatg cctacgaagg gctgtttggc aaagcgccgg
    20581 ttgttgataa gtggaccttc tcaactaacg gcgtatctat catgggccgt cacggcattc
    20641 cggtgatcgg ctttggcccg ggtaaagaac ctgaagcgca tgcacctaac gaaaaaacct
    20701 ggaaatctca cctggtgacc tgtgccgcga tgtacgctgc aatcccgtta agctggctgg
    20761 caacagagta attactcttc acttattccc ctccggttcg ccggagggtt tttggagttt
    20821 gctatgcgcg tattgatcaa aaacggcact gtcgttaacg cagatggaca agccaaacag
    20881 gatttgctga ttgaaagcgg gattgttcgc cagttgggca acaatatttc gccgcagctc
    20941 ccgtatgaag aaattgatgc cactggctgt tacgttttcc ctggcggcgt ggatgtccat
    21001 acgcatttca atattgatgt cggcatcgcg cgcagttgtg atgatttttt taccggtacc
    21061 cgcgcagctg cgtgtggcgg tacaacaacc attattgacc atatgggatt tggcccaaac
    21121 ggctgtcggt tacgccatca actggaggtt tatcgtggtt atgccgccca taaagcggtc
    21181 atcgattaca gctttcacgg tgtgatccag cacattaatc acgcaatcct cgacgaaatc
    21241 ccgatgatgg tcgaggaagg actgagcagt tttaaactct atttaaccta tcaatacaaa
    21301 ctcaacgatg acgaggtttt gcaggcatta cgccgtctgc atgaatccgg cgcgctgacc
    21361 accgtgcacc cggaaaatga tgcggctatc gccagcaagc gggcggaatt tatcgccgca
    21421 gggttaaccg cgccgcgcta tcatgccttg agtcgccctc tggaatgcga agcggaagcc
    21481 atcgcccgca tgattaacct ggcacaaatt gccggtaacg ccccgctcta tatcgtgcac
    21541 ctgtctaacg gcttaggtct ggattatctg cgtcttgccc gtgcgaatca ccagccagtc
    21601 tgggttgaaa cctgcccaca atatctcctg ttggacgaac gcagttacga tacagaagac
    21661 ggcatgaagt tcattcttag cccaccgctg cgtaacgtac gcgagcagga caaactgtgg
    21721 tgtggcatca gcgatggtgc gattgacgtg gtggcgaccg atcactgcac cttctcgatg
    21781 gctcaacgcc tgcaaatttc taaaggcgat ttcagtcgct gcccaaatgg cttacccggt
    21841 gtggaaaacc gcatgcagtt actgttttcc agtggcgtga tgacgggacg tataacaccg
    21901 gaacgctttg ttgaattaac cagcgcaatg cccgccaggc tgtttggcct gtggccgcaa
    21961 aaaggattat tagcgcccgg ttccgatggc gacgtggtga ttatcgaccc acgtcagagc
    22021 caacaaattc agcatcgcca tctccacgac aacgccgact actcgccatg ggagggtttt
    22081 acctgtcagg gcgcgattgt cagaacctta tcccgtggtg aaacgatttt ctgtgacggc
    22141 acctttacag gcaaagccgg gcgaggtcgt ttcctgcgac gcaaaccgtt tgtccctccc
    22201 gtgctctaac cactgtcagg gatctataca caatcaagaa tgatgagtga ggaaaaatga
    22261 gtaagaaaat tgttctcgcc ctgggcggga atgcgctggg cgatgacctg gccggacaaa
    22321 tgaaagcggt aaaaattact tctcaggcaa ttgttgattt aattgcccaa ggacatgaag
    22381 ttatcgtcac tcatggtaac gggccgcagg tgggcatgat taaccaggcc tttgaagctg
    22441 ctgcaaaaac tgaagcgcac tcgccaatgc tgccgatgtc tgtctgcgtt gccttaagcc
    22501 agggttatat tggttacgat ctacaaaacg ccttaagaga agaactgctt tctcggggca
    22561 ttaataaacc ggtagcaacg ctggttactc aggtcgaagt cgacgctaac gatccggcat
    22621 tcctcaaccc gaccaagccg atcggttcgt tctttaccga gcaagaagca gagcaactga
    22681 caaaacaggg ttacactctg aaagaagatg ctggtcgcgg ctatcgccgt gttgttgcct
    22741 cgccaaaacc ggttgatatt attgaaaaag aaacggttaa agctctggta gatgcaggcc
    22801 aggtggtgat caccgttggc ggtggtggta ttcctgttat tcgtgaaggt aaccatctgc
    22861 gtggtgccag cgcggttatc gataaagact gggccagcgc ccgtttagca gaaatgattg
    22921 atgccgatat gctgatcatt ctgactgcgg tagaaaaagt ggccattaac ttcggtaaag
    22981 agaatgaaca gtggctcgat cgcctgtcat taagtgatgc tgaacgcttt atcgaagaag
    23041 gtcattttgc gaaaggttct atgctgccta aagtggaagc tgccgcctcg tttgctcgct
    23101 ctcgcgcagg tcgtgaagca ctgattaccg tattgagcaa agcgaaagag gggattgaag
    23161 gcaaaaccgg aacggtgatt tgtcagtaat tttaaggccg ggtgggtaat accatccggc
    23221 cattttcatt taagccactt ccagcacttc ttttgtcgct ttcacgccct gatgcatcaa
    23281 catcattaac gcctcaagta cgccgccgcc aatcgcccgg gctttatcag aaacgctggt
    23341 gaaatcagcc gtttcaccac gaggatcgat atcaccaatt ttaaaaccac cgaccactgc
    23401 caggccgtcg ttcaacaagc cacgcaccat ccccgtcaac ggtgctttaa tttcatgctc
    23461 accaatccag gcaatcacat cgccctcttt caccagatcg cctaatttca cgttggatcg
    23521 cataatgcct gcagcaggag cacggatcac ccgtcgggtg gtatgcccca taatattgcc
    23581 aggaacaccg gtattctcct gcgcacaacc agagtaaatc acctgaccga gccagtgccc
    23641 gcgatttgtt tcaattaccg catgacaatc cttccctgca gtaaagcccg gcccaagcgc
    23701 gattgttact ggtgccatat ctgcccgcgt tcccaaattc tgtttcgcca gaatagcgtc
    23761 caccacgcaa agcggtttca gttcatcaag caatgaacag gcgggatcta ccatcacagg
    23821 gatgaatccg cgttcggtaa gtttcatcgc ttccgcagag ctggttgcca ggcgagcagt
    23881 gacgccttcg accgtcattt cgccatcgaa cacggcctgg gcaaacgcca ccgtacaacg
    23941 aatcactgtc ggtttttcca cttccagcat gatcacttta aaacccgcat gatacagacg
    24001 tagcgccaca ccactggcga tatctcccgc accgcgaatc accaccagct ggtgtccgga
    24061 aggatgagaa ggtttcatca tcagcccacc cggcgcgtta tttttcacct gtaatatttc
    24121 tgccaacacg ctgatggcga tctcctgcgg cgtttccgca cctatgttgt aaccaacggg
    24181 cgcatgtaaa cgggcaatat gttcttcagc cacgcctttc tcacgcaatt gacgcaggaa
    24241 aagctgaacc ttgcggcgac ttgccagcaa cccaaaccat gcaatgggct gttcaatgag
    24301 tttgtcgagg gcttcacgat cctgattatt cgtggcaatc aggacaaaat tatcagggcg
    24361 aatatccagt gcttccactg ctgcaccaaa cgactcagca tggagaagcg tggttgatgg
    24421 agggaatagt tcaggattga ggctttcgcg ataaatatcg gcaacggcaa tatcaaatcc
    24481 taatagcgcc gcactctggg ctatcgcccg gttgacatgc cccgcgccaa tcaacaccag
    24541 acgtggacgc ataccatgga cgctgataaa tactgacatt gcacctccac aatctgaccc
    24601 gacagcatcc gcaccgttac gagccatacg tccatggaat aatcgcggct tacgttcctg
    24661 caatgcctga agtgactctt caatcacctt ccgctcaacc attccgccac caattgtacc
    24721 gacgatagaa ccatcggcgc gcactaacat ttgtgcagaa tggcggggag tcgagcctcg
    24781 gctatcaaca atttgcgcca tcgcaaacgg acaattttgc tcttcgagtt ttgcagcctc
    24841 tgtgaaaata ttcataccaa cctcaatgga tatcctttca gtaacccgga atacccgggc
    24901 cgctaattca caaatctgcg cgcgattgca ggatgttctt gtatatcgcc cagccagatt
    24961 gcctctacgt cgtgttgttg cagcggttga aggagctcgc tttgtgcaat cgcattctca
    25021 cattgagaaa aacggttaat gaaccagact cgccgacaac cttgcggtac gtttttaaac
    25081 gccccctgag ggtggcgaac cagcgcaacg agatcgctca gttgcaaggt tgcatcaggt
    25141 gttaacccgg taatatcagc aaactgcgac cagcgatgga cattttctgt gctcactttc
    25201 gcgcccaaag tatgtcctcc catcacagca atcacgcagc aactgctttt gggtatgcaa
    25261 ggttcgtgct catcaggcgc ttttaacggc attccacgcg agccatctgc ttcaatgaga
    25321 attacgtcac actctggtcg ttgcaccagt gcatcaatcg cttctggcgt aaatccctgc
    25381 acttttccct ggttcgcttt ccagctgtga aaacaaaatg aaatggggga tgtaagagac
    25441 gcatgaggaa gcatggcggg atcacgacag aaaaccacgg gccagtgaga cgttggcata
    25501 aacatatgtg tcgtcgtcgt gattaacaca cgcctgccgc tagcctggag cagttctgcc
    25561 agccaaaaaa gcaagctggt tttcccccca gcaccaacaa cagaaatcac agtgggacgt
    25621 ttctgcgcac ctaaatcaat gactaatgcc gatgggtcaa ctatactttt cacgagtctt
    25681 tatgacctct ctgggataaa ttatccccaa cttaatccat caggaagtaa cgcaattatc
    25741 aggcgttatt agcccctata aataatggaa ccactatgtc agccatcgac tgtataatta
    25801 ccgctgccgg attatcatca agaatggggc aatggaaaat gatgttaccc tggcaacagg
    25861 ggacaattct tgatacaagt atcaaaaatg cgttgcagtt ttgtagccga attattttag
    25921 tcaccggcta tcgtggtaat gaactgcacg aacgttatgc gaaccagagc aatatcacta
    25981 ttatccacaa cccagattat gcgcagggtt tactaacatc agtaaaggcc gcagtacccg
    26041 cagtgcaaac agaacattgt tttctcaccc acggtgacat gccaaccctc accatcgata
    26101 tttttagaaa aatctggtcg ttacgaaatg atggcgcaat actgccactc cataatggca
    26161 tccccggcca tccgatttta gtgtcaaaac catgcttgat gcaggcaatc caacgaccca
    26221 atgtcaccaa tatgcgtcag gcactcctta tgggagctca ttattccgtc gagatagaaa
    26281 atgcagaaat aattttagat attgatactc cggacgactt tattacagcg caaaaaaggt
    26341 atactaaaat ttagaaaatg tagttaaacg attgcgttca aatatttaat ctctccagca
    26401 ctctttatca taatgataag gaatcactga ttttgagaaa agagaaaaag tgataaatgg
    26461 gctgacaata ataattttca ggagtgattt taattctcat ttggcacctg tgagttcatt
    26521 cacaaaaagc ctttaagttt cgagccgcct cacatttttt tatatttccc gccaaacctg
    26581 gcaagagtgg tgcgattgtt gctctatccc cctaaaccac cggatttctc aacaccggtc
    26641 actcaatgat atctgtataa gctaaggaga gggttatggg ggatattatg cgtcccattc
    26701 cgtttgagga acttttgacg cgcatatttg atgaatacca acaacaacgc tcaatctttg
    26761 gtattcccga gcaacagttt tactcacctg taaaaggtaa aactgttagc gtcttcggtg
    26821 aaacctgtgc cactcccgtc ggccctgccg ctggcccgca cacgcagctc gcgcaaaata
    26881 ttgtcacttc ctggctgact ggcggacgct tcatcgaact aaaaaccgtc caaattcttg
    26941 accgcctgga gctggaaaag ccctgtatcg atgccgaaga cgagtgcttt aacaccgaat
    27001 ggtctaccga atttaccctg cttaaagcct gggatgaata cctcaaagcc tggtttgccc
    27061 tgcaccttct cgaagcgatg ttccagcctt ctgattccgg taaatcgttc atctttaata
    27121 tgagcgtcgg ttacaacctc gaaggtatta agcaaccgcc aatgcaacag ttcatcgaca
    27181 atatgatgga cgcatctgac catccgaaat tcgcgcaata tcgcgatacg ctgaataaat
    27241 tactccagga tgacgcattt ttagcccgcc acggattgca ggaaaaacgc gaaagcttgc
    27301 aagccttacc cgctcgcatc cccaccagta tggtgcaggg cgtcaccctc tccaccatgc
    27361 acggctgtcc tccgcatgaa atcgaagcca tttgccgcta catgctggaa gaaaaagggc
    27421 tcaacacctt tgtgaaactt aacccgacct tactgggata cgcgcgtgtt cgtgagatcc
    27481 tcgatgtctg cggtttcggt tacataggct taaaagaaga gtcatttgat cacgacctca
    27541 agctgacgca agcactggaa atgctggaac gcctgatggc actggcaaaa gaaaaatcac
    27601 tcggctttgg cgtaaaactg actaacactc tcggcaccat caacaataaa ggcgcactgc
    27661 ctggtgaaga gatgtatatg tcaggccgtg cgctgttccc gctctccatc aatgttgcag
    27721 cagttctctc tcgcgccttt gacggcaaac tgcccatttc ttattccggt ggtgccagtc
    27781 agctgactat ccgcgatatt tttgatacag gtattcgccc tattactatg gcaaccgacc
    27841 tgctgaaacc tggcggctat ttgcgcttaa gtgcctgcat gcgcgagctg gaaggctccg
    27901 acgcctgggg acttgaccat gttgacgtcg aacgactgaa cagactggca gcagatgcgt
    27961 taaccatgga atacacccag aaacactgga agccagaaga gcgtattgaa gtggcagaag
    28021 acctgccgct gaccgattgc tacgttgccc cctgtgttac tgcctgcgct atcaagcaag
    28081 atattccgga atacatccgt ctgcttggcg aacaccgcta tgccgacgcg ctggaactca
    28141 tctaccaacg caacgctctg cccgccatta ccggtcatat ttgcgatcac cagtgccaat
    28201 acaactgtac ccgcctggat tacgacagtg cgctgaatat ccgcgaactg aaaaaagtcg
    28261 cgctggaaaa aggttgggat gaatataagc aacgctggca caaacccgtc ggttctggtt
    28321 cacgccatcc ggttgccgtg attggtgcag gtccggcggg tctggcagca ggttacttcc
    28381 ttgccagagc gggccatccg gttacgctgt ttgaacgcga agccaatgct ggcggcgtgg
    28441 tgaaaaatat cattcctcag ttccgtattc ctgcagagtt aattcagcac gatatcgatt
    28501 ttgttgccgc tcacggcgtg aaatttgagt atggctgctc acccgatttg accgttgaac
    28561 agttaaaaaa tcagggcttc cactatgttc tgattgccac cggcactgat aaaaatagcg
    28621 gtgtgaaact ggcgggcgac aaccaaaatg tctggaaatc actccccttc ctgcgtgaat
    28681 acaacaaggg tacagcgctc aagctgggca aacatgtggt cgttgtcggg gcgggtaaca
    28741 ccgcaatgga ctgcgctcgt gcggcgttac gcgttccagg cgtagaaaaa gcaacgatcg
    28801 tttaccgtcg ttcactgcaa gagatgcccg catggcgcga agagtatgaa gaagcgttgc
    28861 acgacggcgt ggagttccgt ttcctgaata atccggaatg tttcgatgct gatggcacct
    28921 taaccttgcg cgttatgtcg cttggcgaac ccgatgagaa aggtcgtcgc cgtccggttg
    28981 aaaccaacga aacagtaaca ctgcatgtag acagtctgat caccgccatc ggtgaacagc
    29041 aggatactga agccctgaat gcgatgggcg taccgctgga caaaaacggc tggccagacg
    29101 tcgaccataa tggcgaaact cgtctgactg acgtctttat gatcggcgac gtacagcgcg
    29161 gaccatcctc cattgtcgct gctgtcggaa ccgcgcgtcg ggcgaccgat gccatcctta
    29221 gtcgggaaaa tatccgttcc caccagaaca ataaatactg gaacaacgtc aatccggcgg
    29281 aaatctatca acgtaaaggc gatatctcta tcacgctggt gaacagtgac gatcgtgatg
    29341 cgtttgtcgc gcaggaagct gctcgctgcc ttgaatgtaa ctacgtttgc agcaagtgcg
    29401 tggatgtctg cccgaaccgc gccaacgtct ccattgcagt ccctggcttc cagaatagat
    29461 tccagacgct gcacctcgac gcttactgta acgaatgcgg caactgcgct cagttctgtc
    29521 cgtggaacgg taaaccatac aaagacaaaa tcaccgtctt cagcctggcg caagactttg
    29581 ataacagcag caacccaggc ttccttgtgg aagattgccg ggtacgagta cgtctgaata
    29641 accaaagctg ggtgttaaac atcgacagcg aaggtcagtt taacaacgta ccaccggagc
    29701 tgaacgatat gtgccgcatc atcagccatg tccaccagca tcatcattat ctgctgggcc
    29761 gcgtggaggt gtaatcatgt tgattctgaa gaatgtcacc gcagtgcagt tacacccggc
    29821 gaaagtgcag gaaggcgttg atatcgctat cgaaaatgac gtgattgtcg ctatcggcga
    29881 tgccctgacg caacgctacc ccgatgccag ctacaaagag atgcatggtc ggattgtgat
    29941 gccgggaatt gtctgctcgc acaaccattt ttactcgggg ctttcccgcg gaattatggc
    30001 aaacatcgcc ccctgcccgg atttcatctc aacgctgaaa aatctctggt ggcggctcga
    30061 tcgcgccctt gatgaagagt cgctctatta cagcggactg atttgttccc tggaagcgat
    30121 taagagcgga tgtacatcgg ttatcgatca ccatgcctct ccggcgtata tcggcgggtc
    30181 gctctccaca ttgcgcgacg catttttaaa agttggcctg cgcgcaatga cctgttttga
    30241 aactactgac cgtaacaacg gcatcaaaga gttgcaggaa ggtgtagaag aaaacatccg
    30301 ctttgcccgt cagattgatg aggcgaagaa agcagcaacc gagccgtatc tggtggaagc
    30361 acatatcggt gctcacgcgc cgtttaccgt gccggatgcc ggtctggaga tgctgcgtga
    30421 agccgtgaaa gccacaggcc gtggtttgca tattcacgct gcggaagacc tttacgacgt
    30481 ttcctacagt caccactggt acggcaaaga cctgctggca cgactggcgc aattcgatct
    30541 catcgacagc aaaacgctgg tcgctcatgg gctgtacttg tcgaaagatg acatcaccct
    30601 actcaatcag cgcgatgcgt tcctggtgca taacgcccgt tcaaacatga acaaccatgt
    30661 cggctacaac catcacctta gcgacatccg caatctggcg ttgggaacgg acggcattgg
    30721 ttcggacatg tttgaagaga tgaaatttgc cttctttaaa catcgcgatg cgggtggtcc
    30781 gctgtggcct gacagttttg ccaaagccct gactaacggt aacgaactga tgagccgcaa
    30841 ctttggcgcg aaatttgggc ttctggaagc cggttacaaa gctgatttaa ccatttgcga
    30901 ttacaactcg ccgacgccgc tgctggcaga caatatcgcc gggcatatcg ctttcggtat
    30961 gggctcaggc agcgttcaca gcgtaatggt caatggtgtg atggtctatg aagaccgtca
    31021 gtttaacttc gattgcgatt ccatttatgc acaagccaga aaagccgctg ccagtatgtg
    31081 gcgtcggatg gatgcgctgg cataaatgac agatgccctc ttcccgcagg gaagagggtt
    31141 aaataaggaa agaagatgat tgaacaattt ttcaggcccg actctgtcga acaggcgctg
    31201 gaactgaagc gccgctacca ggatgaagcc gtctggttcg ccgggggcag caaactcaat
    31261 gctacaccaa cccgtaccga taaaaagatt gccatttcct tacaggatct ggaactggac
    31321 tgggttgact gggataacgg tgcactgcgg attggcgcaa tgtctcgctt gcagccactg
    31381 cgtgatgcgc gatttattcc tgcagcgctg cgtgaagccc tcggttttgt ttactcacgc
    31441 catgttcgta atcagtcgac cattggtggt gaaatcgccg cccgccagga agagtcggtg
    31501 ctgcttcccg tcctgttggc actggatgct gaactggttt ttggcaacgg cgaaacgctg
    31561 tcaatcgagg actacctggc ctgcccatgc gatcgcctgc taaccgaaat tatcattaaa
    31621 gatccgtatc gcacctgtgc gacccgcaaa attagccgtt ctcaggcagg tttaaccgtc
    31681 gtgacggcag ccgttgcaat gacagaccac gacggtatgc gaattgcgct ggatggcgtc
    31741 gccagtaaag cactgcgtct gcatgatgtc gaaaaacaaa atctggaagg caatgcactt
    31801 gaacaggctg tcgccaacgc cattttcccg caggaagatt tgcggggcag cgtggcctat
    31861 aaacgctata tcacgggagt tctggtagcc gacctgtatg ccgactgcca acaggctggg
    31921 gaggaagccg tatgatcatc cactttactt taaatggcgc gcctcaggag ctaaccgtta
    31981 atccaggcga aaacgtgcaa aagctgttgt ttaacatggg aatgcactct gtacgcaaca
    32041 gtgatgatgg tttcgggttt gccggttctg acgcaataat ctttaacggt aatatcgtta
    32101 acgcgtcctt gcttattgcc gcacagttag agaaggcaga tattcgtacc gcagaatctc
    32161 tgggcaaatg gaacgagtta agtctggttc aacaggcaat ggttgatgtt ggcgtggtgc
    32221 agtctggtta taacgatcca gctgcagctc tgattatcac cgatcttctc gatcgcatcg
    32281 ccgcacctac ccgcgaagag atcgacgacg cgctttctgg tttgttcagc cgcgatgctg
    32341 gctggcagca atactatcag gtcattgaac tggcggttgc acgtaaaaat aatccgcagg
    32401 ccaccattga tatcgctccg actttccgtg acgacctaga agtcattggt aagcattatc
    32461 ctaaaactga tgccgcgaaa atggtgcagg cgaaaccctg ctatgttgaa gaccgcgtaa
    32521 cggctgacgc ctgcgtcatt aaaatgttac gtagcccaca cgctcacgca ctgattactc
    32581 atctggatgt cagcaaagct gaagccttac cgggcgtcgt tcacgttatt actcacctga
    32641 attgcccgga tatctactat accccgggtg gtcagagcgc accggaaccg tcaccgcttg
    32701 accgccgtat gttcggcaag aaaatgcgtc acgtcggcga tcgtgttgct gcggtcgtcg
    32761 cagaaagtga agaaattgcg ctcgaagcac tgaagctcat cgacgttgaa tatgaagtgc
    32821 ttaagccggt aatgtcgatc gacgaagcaa tggcggaaga tgcgcctgtc gtgcacgatg
    32881 aaccggtggt gtatgttgct ggtgcgccag atactctgga agacgataac agccatgcag
    32941 cccagcgcgg cgagcatatg atcatcaact tcccgatcgg ttctcgccct cgcaaaaata
    33001 tcgccgccag tattcatggt catattggcg atatggacaa aggctttgcc gatgccgatg
    33061 tgatcattga gcgaacctat aactcaacgc aagcgcagca gtgcccgact gaaacacata
    33121 tctgctttac tcgtatggac ggcgatcgtc tggttatcca cgcctccacc caggtaccat
    33181 ggcacttacg ccgccaggtc gcgcgcctcg tgggcatgaa acagcataaa gttcatgtca
    33241 ttaaagagcg agttggcggc ggttttggtt ccaaacagga catcctgctg gaagaagtgt
    33301 gcgcctgggc aacctgcgtg accgggcgtc cggtactgtt ccgctacacc cgtgaagaag
    33361 agtttattgc taacacctct cgtcacgtcg cgaaagtcac cgtcaaactg ggagcgaaaa
    33421 aagatggtcg cctgacggca gtgaagatgg atttccgcgc caacactggc ccttacggca
    33481 accactcact caccgtaccg tgtaacggac cggcgctgtc gctgccgtta tatccgtgcg
    33541 ataacgtcga tttccaggtc accacctact acagcaacat ttgcccaaat ggtgcttatc
    33601 agggttatgg cgcaccgaaa ggtaacttcg ctatcaccat ggcattagcg gaactggctg
    33661 aacagttaca gatcgaccaa ctggaaatta tcgaacgtaa ccgggtacac gaagggcaag
    33721 agctgaaaat tctcggtgca atcggtgaag gtaaagcgcc gacctccgtt ccttccgccg
    33781 ccagctgcgc actggaagag atcctgcgtc agggtcgcga gatgatccaa tggtcttcac
    33841 cgaaaccgca aaatagtgac tggcacatcg gtcgcggcgt cgccattatc atgcagaaat
    33901 cgggtatccc ggatatcgat caggctaact gcatgatcaa actggaatcg gacggcacct
    33961 ttatcgttca ttctggcggt gcggatattg gtactggtct ggatactgta gtgacgaaac
    34021 tggcagcaga agtgctgcac tgcccaccgc aggacgtgca tgttatctcc ggtgataccg
    34081 atcatgcgtt gtttgataaa ggcgcatatg cctcgtccgg tacttgcttc tcgggtaacg
    34141 cggcgcgttt ggcagcggaa aatctgcggg agaaaattct gttccacggc gcgcaaatgt
    34201 tgggtgaggc agtggcagat gttcaactgg caacgccggg cgtcgtgcgc ggcaagaaag
    34261 gcgaagtcag cttcggggat attgctcata aaggcgaaac cggcaccggc tttggttcac
    34321 tggtgggaac tggcagttat atcacgcctg atttcgcctt cccgtatggc gcaaacttcg
    34381 ctgaagttgc cgtcaacacg cgtacgggtg aaatccgcct ggataaattc tacgccttgc
    34441 tggactgcgg tacaccggtc aacccggaat tagcattggg acaaatctac ggtgctaccc
    34501 tgcgagctat cggccacagt atgagcgaag agatcattta tgacgccgaa ggtcacccgt
    34561 taacgcgtga tttacgcagt tacggcgcac cgaaaattgg tgacattccg cgtgatttcc
    34621 gcgctgtgct ggtgccgagc gacgataaag tcggcccgtt cggggcgaaa tcgatctcgg
    34681 aaatcggtgt aaatggcgca gctccggcga ttgctaccgc aattcacgat gcatgcggca
    34741 tctggttacg cgaatggcat ttcacaccgg agaaaatact cactgcgctg gaaaaaatat
    34801 aaataataaa tggccgcttc ggcggcctgt ttctctgttt ggacgatgac taaatagtga
    34861 ctgcgaaaat gagataagcc gttctcaagg cgtcctgcgc cccaaattcg tgggaattta
    34921 ttttactaat tcagatgatc aaatttactt taaaaggagt gaagggatgt ctgatataaa
    34981 caatgcaggt tctgacctta tatttgaact ggaggatcgc cctccctttc atcaggctct
    35041 cgttggtgcc attacccatc tgttggcaat tttcgttccg atggtaaccc ccgcgttaat
    35101 cgtgggggcg gccttacagc tttccgctga aacaactgcc tatcttgttt ctatggcgat
    35161 gatcgcctct ggtattggta tctggttaca agtaaaccgc tacggcatcg tcggttctgg
    35221 cctactctca attcagtcag tcaatttttc atttgttacg gtcatgattg cgctgggcag
    35281 cagcatgaaa agcgacggtt ttcacgaaga gttaatcatg tcgtcgcttc tcggcgtctc
    35341 cttcgttggc gcatttctgg ttgtcggatc ttcatttatc ttgccctatt tacgtcgggt
    35401 tattacgcct accgtcagcg gtattgtggt actgatgatc ggcttaagcc tgattaaagt
    35461 cggcattatc gattttggtg gaggatttgc agccaaaagc agcggtacat tcggcaatta
    35521 cgaacatctc ggcgttggtt tattggtttt aattgtggtg atcggcttta actgctgtcg
    35581 cagtccgttg ctacgcatgg gagggatcgc cattgggcta tgtgtcggct atatcgcatc
    35641 gttatgcctg ggcatggtgg atttcagcag tatgcgcaat ttgccgttaa tcaccatccc
    35701 gcatccgttc aaatacggct ttagttttag cttccatcag ttcctggtgg ttggcacgat
    35761 ttatctgctt agcgtgctgg aagcagtcgg cgatatcacc gccacggcaa tggtttcccg
    35821 ccgccccatt cagggggaag agtatcagtc ccgactgaaa ggcggcgtgc tggcagatgg
    35881 tctggtttct gttatcgcct ccgctgtcgg ttcattacca ttaaccacgt ttgcgcaaaa
    35941 taatggggtt attcagatga ctggcgtcgc ttcacgttat gtcgggcgaa ccatcgcggt
    36001 aatgctggtt atcctcggct tatttccgat gattggcggc ttcttcacga ccattccctc
    36061 ggcagttctg ggaggcgcaa tgacgttgat gttttccatg attgccatcg cagggattcg
    36121 catcatcatc accaacggtt taaagcgccg tgaaacactt attgtcgcca cttctttagg
    36181 tttagggctt ggcgtctcct acgatcccga aatttttaaa atattgccag cctctattta
    36241 tgtattagtt gaaaacccta tttgtgctgg cgggttaact gcgattttat taaatattat
    36301 cctccctggt ggctaccgac aggaaaacgt tctgcctggt attacctcag cggaagagat
    36361 ggattaacag taaaggagtc aatgatgtca ggagaacaca cgttgaaagc ggtacgaggc
    36421 agttttattg atgtcacccg tacggtcgat aacccggaag agattgcctc tgcgctgcgg
    36481 tttattgagg atggtttatt actcattaaa cagggaaaag tggaatggtt tggcgaatgg
    36541 gaagacggaa agcatcaaat tcctgacact attcgcgtgc gcgactatcg cggcaaactg
    36601 atagtaccgg gctttgtcga tacacatatc cattatccgc aaagtgaaat ggtgggggcc
    36661 tatggtgaac aattgctgga gtggttgaat aaacacacct tccctactga acgtcgttat
    36721 gaggatttag agtacgcccg cgaaatgtcg gcattcttca tcaagcagct tttacgtaac
    36781 ggaaccacca cggcgctggt gtttggcact gttcatccgc aatctgttga tgcgctgttt
    36841 gaagccgcca gtcatatcaa tatgcgtatg attgccggta aggtgatgat ggaccgcaac
    36901 gcaccggatt atctgctcga cactgccgaa agcagctatc accaaagcaa agaactgatt
    36961 gaacgctggc acaaaaatgg tcgtctgcta tatgcgatta cgccacgctt cgccccgacc
    37021 tcatctcctg aacagatggc gatggcgcaa cgcctgaaag aagaatatcc ggatacgtgg
    37081 gtacataccc atctctgtga aaacaaagat gaaattgcct gggtgaaatc gctttatcct
    37141 gaccatgatg gttatctgga tgtttaccat cagtacggcc tgaccggtaa aaactgtgtc
    37201 tttgctcact gcgtccatct cgaagaaaaa gagtgggatc gtctcagcga aaccaaatcc
    37261 agcattgctt tctgtccgac ctccaacctt tacctcggca gcggcttatt caacttgaaa
    37321 aaagcatggc agaagaaagt taaagtgggc atgggaacgg atatcggtgc cggaaccact
    37381 ttcaacatgc tgcaaacgct gaacgaagcc tacaaagtat tgcaattaca aggctatcgc
    37441 ctctcggcat atgaagcgtt ttacctggcc acgctcggcg gagcgaaatc tctgggcctt
    37501 gacgatttga ttggcaactt tttacctggc aaagaggctg atttcgtggt gatggaaccc
    37561 accgccactc cgctacagca gctgcgctat gacaactctg tttctttagt cgacaaattg
    37621 ttcgtgatga tgacgttggg cgatgaccgt tcgatctacc gcacctacgt tgatggtcgt
    37681 ctggtgtacg aacgcaacta ataataaaac tttaacatcc tcgtgaggac atcattatgt
    37741 ctggagacat cctacaaaca ccggacgcac caaagccaca gggcgcgctg gataattatt
    37801 ttaaaattac cgctcgtggc agtaccgttc gtcaggaagt actggctggc ttaacgacct
    37861 ttctggccat ggtttattcc gttatcgtcg ttccgggaat gctgggcaaa gcaggttttc
    37921 ctcccgcagc tgtgtttgtt gccacctgtc tggtcgcggg cttcggctcg ttgctgatgg
    37981 ggttgtgggc caatttgcca atggcgattg gttgtgcgat ttccttgacg gcatttaccg
    38041 cattcagtct ggtactcggg caacaaatta gcgttcctgt cgcactgggc gcggtctttc
    38101 tgatgggcgt catcttcacc gctatttccg taaccggtgt gcgtacctgg atcttacgta
    38161 atttaccgat gggtatcgct cacggtacag gtatcggtat cgggctgttt ctgctgctga
    38221 ttgctgctaa cggtgtgggt atggttatca aaaacccgat tgaaggcttg ccagtggcgc
    38281 tcggtgcgtt tacctccttc ccggtgatga tgagcttgct ggggctggcg gtcatcttcg
    38341 gtctggagaa gtgtcgcgta cccggcggga tcttgttggt gattattgca atttcgatca
    38401 tcggcttaat ctttgaccca gcggtgaaat accacggtct ggtggcgatg ccaagcctga
    38461 ctggcgaaga tggtaagtct ctgattttca gcctcgatat tatgggcgca ctccagccaa
    38521 ctgtacttcc gagtgtactg gcattggtga tgaccgcagt gttcgacgct actggcacca
    38581 tccgtgccgt cgccggtcag gcgaatttgt tggataaaga caaccagatc atcaacggcg
    38641 gcaaagccct gaccagtgac tcagtaagtt caatattctc cggcctggtg ggcgcagcgc
    38701 ccgcagcggt ttatatcgaa tcagcggcag gaaccgccgc cgggggtaaa acagggttaa
    38761 ccgcaaccgt agtgggggcg ttattcctgc tgattctgtt cttatcaccg ctgtcatttt
    38821 tgatccctgg ttacgccact gcacccgctc tgatgtacgt aggtttgctg atgttaagta
    38881 acgtctcgaa gctggatttc aatgatttta ttgacgctat ggctggcctg gtgtgtgccg
    38941 tgttcatcgt tctgacttgt aatatcgtta ccggtattat gctgggcttt gtgacactgg
    39001 tcgtaggccg cgtctttgca cgcgaatggc aaaagctgaa tattggtacg gtgatcatta
    39061 ctgccgcact ggtcgcattt tacgcgggtg gttgggcaat ctaatagatt ctccgcgcct
    39121 ttcagcgcgg agatttcttt cacagaggat tctcaccgct ggcaacaata cgctgctggc
    39181 ggactcgcct gagttctctt tcagtaagca actgcagcgc ctgtgttggg caagattcaa
    39241 cgcaggcagg cccctcttcc cgctgttcac agagatcgca tttcacaatt tgttgtcggg
    39301 tatcccccgg caacgactga atggtgatca ttccaaacgg gcacgcactg acgcaactct
    39361 gacagccaat acaacgggca gaattggtct gcaccacctg ctcgcccatc gtcagcgccc
    39421 ccacggggca agcgccaaca caaggggcgt tttcacactg atggcacatc accggcgcac
    39481 tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa gacatcggca ttcaactcct
    39541 gttctgaagg gtgagcgacc acacaggcta cttcacaagt acggcagcca atacagtcag
    39601 ccggattaac gataattaac gatttcatca cgatgccttc gtatcaaaca gagttaacat
    39661 atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca accagatccg cgccatgaac
    39721 tgcatcccca ccagcaaaga cttttttcag atgcgtctgg gtaggtaaat acccgacgtc
    39781 accggtttga atcaggcccc atttatcgag tttaattccg ctgccctgca accacggcat
    39841 ggcatgcgcc tggaaaccaa aggccataat cagaacatcg gcgggcaatt caaactctga
    39901 acccgctacc ggacgaggac gacggcgacc atccggcccc ggctcaccca tggcggtacg
    39961 aatcaggccc accgcagtta aacgcccatc ttcgtcacaa gcgatatatt gcggttgaac
    40021 attgaactga aactcgacac cttcctcgca cgcattaacc acctctttgc gcgagcccgg
    40081 catactgact tcatcacgac gatacgcgca ggtcacgctg gcagcattga ggcgaatgga
    40141 agtccgcaaa caatccattg tcgtatcgcc accgcccaat accacgactc gcttaccttc
    40201 cacgtccgtc agcggatact cttcagactc cggcaatccc atgagctggc gggtatgggc
    40261 agtcaggaac ggtagagcct gaatgacacc gggtgcatct tcatgcggca gatctgctcg
    40321 catcatcccg taagtcccca cgccgatgaa aactgcatca tattcagaag ttaaatcgct
    40381 aaaggtaata tcgcggccaa tttcacagtt aagatgaaag tcgattccca ttgcggtgaa
    40441 tatctctcgc cgctggctta ataccgtttt atcgagtttg aaaggaggaa tgccaaaagt
    40501 cagcataccg ccaatttctg gatggcgatc aaagacatca acctgaactc ccgcgcgcgc
    40561 cagaatatca gcacacccta accctgctgg cccagcgcca atcaccgcca ctttttcgct
    40621 acggggaaca actttgctga catcgggacg ccagcccatc gccagcgcgg tatcggtgat
    40681 gtagcgttcc agattaccga tagagactgc gccagagtga tctttcaaag tacatgcgcc
    40741 ttcacaaaga cggtcctgtg ggcataccct gccgcagatt tctggtaagg aactggtctg
    40801 gtggcaaagt tctgccgctt caataatctt tccttcctgt accagacgga tgtaatccgg
    40861 aatagcgtta tgcagcgggc aatgccagtt gcagttagct ttttcggcac aataaacaca
    40921 gcggtcactc tcataagtcg cttgcagtgg atccagcccg caatagattt cgccaaagtg
    40981 ggttttccgt tcactcgctg aaattttatc tgcgccttta cgcgagttaa cggggagcaa
    41041 cgctgcactg cgagatggct gagcgactga tgacgctttt cctgctgccg ttttgcgctg
    41101 gcgggccacc tttatctgct gtaacccttt atcgtccatc agtcgtaacg cctgtgttgg
    41161 gcagacttca atacaggctt gcgtgccaga actgcgctgg ttacaaaggt cgcatttctg
    41221 cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa gcgattgcgc atcttttaca
    41281 accaatacat ttttgctcgt ccagttgtac gctatcggac tggaaagtca gagcattaac
    41341 cggacaagcc gtaacgcaag gggcattgtt gcagtgatgg caggccaccg gattcgcagc
    41401 ctggcctttc cctacaacgt ggatacgcgg tctaaagtca ctgtgactca gcggccagtt
    41461 ttcttgattg tgtgccaccg cacaggcaat ttcacaagca tgacagccta tacattccgc
    41521 agcttcagca gcgataaact tattcatttg catccctttc atttaatgag ttatgtcttc
    41581 tcaaatttcg cgatgcaata aacgggcata tattaggtag catgaccgtt ttaattataa
    41641 ttttgcgact gagttcaaat tattgcccct caatctatgc cgctttcatt atttcaacat
    41701 tgccgaccaa aagagcgcta tccagataaa aacgattaaa tgcgagagtg cgacatgcca
    41761 gaatgattaa tgaaatatca atatgagaaa attacagata atttcattgt caggttctct
    41821 tctcattttc gtggtttaac cacgattatg tgattgcctc cgcaattcct gtctctaact
    41881 ccccttcctc gcaaaaactg gcactccacg agcatgtgtt tagacagttt cattaacgta
    41941 aacggttgct ttttactctg gcgggcgaaa ggagaaacac tgatgagcgc catagattcc
    42001 caacttccct catcttctgg gcaagaccgc ccaactgatg aggttgaccg catattatca
    42061 ccaggaaagc tgatcatact cggtctgcaa cacgtccttg tcatgtacgc aggtgcagtc
    42121 gctgttcctc ttatgattgg tgaccgactg ggcctctcaa aagaagctat tgcgatgctc
    42181 attagctcgg atctcttttg ctgcgggatc gtcacattat tgcaatgtat cggtatcggc
    42241 cgctttatgg ggatccgcct gccggtgatt atgtcggtga cctttgctgc tgtaacacca
    42301 atgatagcca ttgggatgaa cccggatatc ggcctgctgg ggatatttgg tgccactatc
    42361 gccgcgggtt ttatcaccac attattagcg ccacttatcg gtcgcttgat gcctttattc
    42421 ccgccactgg ttaccggtgt ggttattact tctattgggc ttagcatcat tcaggtgggt
    42481 attgactggg ccgccggagg taaagggaat ccgcaatatg gtaatcccgt ttatttaggt
    42541 atctcctttg ctgtcttaat ttttatcttg ctcattactc gctatgcgaa aggatttatg
    42601 tccaacgtcg ccgtattact ggggattgta tttggctttt tactttcgtg gatgatgaat
    42661 gaagtcaatt tatccgggct acatgatgct tcatggtttg cgattgtcac gccgatgtca
    42721 tttggtatgc cgattttcga tcccgtttcc attctgacca tgactgccgt gttaatcatc
    42781 gtgtttatcg agtcgatggg gatgttcctg gcactgggtg aaatagtcgg tcgtaaactc
    42841 tcttcacacg atattattcg cgggctgcgt gtcgatggcg tagggacaat gataggcgga
    42901 acgtttaaca gcttccccca cacgtcattt tcacaaaacg ttggcctggt tagcgtgacg
    42961 cgcgttcata gccgctgggt gtgtatttct tcgggaatta tattaatcct gtttggcatg
    43021 gtgccaaaaa tggcggtgct ggtcgcctcc attccgcaat ttgtgctggg cggtgctggg
    43081 ctggtgatgt tcggcatggt actggcgaca gggattcgaa ttctgtcgcg ctgtaactac
    43141 accaccaacc gttacaacct ctatattgtg gcgatcagtc tcggcgttgg catgactccg
    43201 acgctctctc acgatttctt ttctaagtta ccggccgtac tgcaaccgtt gctgcatagc
    43261 ggcattatgc tcgcaaccct tagcgccgtt gtgctgaatg tcttctttaa tggctaccag
    43321 catcatgctt acctggtgaa ggaatccgtc tctgataaag atttaaaagt caggacagta
    43381 cgtatgtggc ttctgatgcg caagctgaag aaaaatgagc atggagaata atatgaattt
    43441 attaatgcgc gctatattca gtctgctgtt gctttttact ctctctattc ctgtcatttc
    43501 tgactgtgtt acaatggcca ttgaaagtcg cttcaaatat atgatgctac ttttttaaat
    43561 ggtttttacc tgtcggcatc cgctcaaaac gggcggttgt cgataaacgc tcacttggtt
    43621 aatcatttca ctcttcaatt atctataatg atgagtgatc agaattacat gtgagaaatt
    43681 atgcaaacgg aacacgtcat tttattgaat gcacagggag ttcccactgg tacgctggaa
    43741 aagtatgccg cacacacggc agacaccctc ttacatctcg cgttctccag ttggctgttt
    43801 aatgccaagg ggcaattatt agttacccgc cgcgcactga gcaaaaaagc gtggcctggc
    43861 gtgtggacta actcggtttg tgggcaccca caactgggag aaagcagcga agacgcggtg
    43921 atccgccgtt gccgttatga gcttggcgtg gaaattacgc ctcctgaatc tatctatcct
    43981 gactttcgct accgcgccac cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta
    44041 tttgccgcac gcaccaccag tgcgttacag atcaacgatg atgaagtgat ggattatcaa
    44101 tggtgtgatt tagctgatgt attacacggt attgatgcca cgccgtgggc gttcagtccg
    44161 tggatggtga tgcaggcgac aaatcgcgaa gccagaatac gattatctgc atttacccag
    44221 cttaaataaa aaaccccgac atttgccggg gttgtgagca taacgtaatg cttatttcac
    44281 cggacgcatc gccgggaaca gaataacgtc gcggatggta tggctgttgg tgaacagcat
    44341 taccatacgg tcgatgccaa tccccagacc tgctgtcggc ggtaagccat gttccagtgc
    44401 ggtaacgtaa tcttcatcgt agaacatcgc ttcgtcgtca cctgcgtctt tcgcggcaac
    44461 ctgatccagg aagcgttgcg cctgatcttc cgcgtcattc agctcgctaa agccgttacc
    44521 gatttcacga ccaccgatga agaactcaaa gcggtctgtg atttccgggt taatgtcgtt
    44581 acgacgcgcc agcggagaaa cttctgccgg atattcagta atgaaggtcg gctgaatcag
    44641 atgtgcttct gccacttctt cgaagatctc ggtaacgata cggcccagac cccagctctt
    44701 ctcaacgtgg ataccgatag attcagcaat cgctttcgca gagtcgaagt tatccagatc
    44761 cgccatgtcg gtttccgggc gatatttctt gatcgcttca cgcatggtca gtttttcgaa
    44821 cggtttaccg aagtccagcg tcacgtcgcc ataggtcact tccgtcttac cgagaatatc
    44881 ctgtgccaga gtacggaaca gcgattcggt cagctcgatc agatctttgt aatctgcgta
    44941 agccatgtag agttccatca tggtgaactc tgggttatga cgtacggaga taccttcgtt
    45001 acggaagtta cggttgattt cgaatacacg ctcgaagcca ccgaccacca gacgcttgag
    45061 gtacagttcc ggcgcgatac gcaggtacat gtcgagatcc agcgcgttat ggtgggtgat
    45121 aaacggacgc gcagcggcac cgccagggat cacctgcatc atcggcgttt caacttccat
    45181 aaagccgcgg ttcaccatga actggcgaat accagagagg atctgcgagc gcactttaaa
    45241 ggtgttacgg gattcatcgt tagagatgag atccagataa cgctgacgat agcgcgcttc
    45301 ctgatcctgc aagccgtgga atttatccgg cagcggacgc agtgctttgg tcagcagacg
    45361 cagctcggta cagtggatag acagttcgcc ggttttggtt ttgaacagtt tacctttcgc
    45421 gccgaggatg tcaccgaggt cccatttttt gaactgctcg ttgtaaacgc cttccggcag
    45481 atcgtcacgg gcaacgtaca gctgaatgcg gccacccaca tcctgcaggg taacgaaaga
    45541 cgctttaccc ataatacggc gggtcatcat gcggccagca acggcgactt cgatgttcag
    45601 cgcttccagt tcttcgttct ctttaccgtc gaattctgcg tgcaattggt cagaggtatg
    45661 atcgcgacgg aaatcgttcg ggaaggcaat cccctgctcg cgcaggttcg ccagcttctc
    45721 acgacgcgtt ttcagttcat tgttaagatc gactaccgcg tcagcgccct gtgcgtgttg
    45781 ttcagacatg ttggttcctc ataaccctgc tttcaaactt gcttcgataa attgatccag
    45841 gctgccgtcc agcacggcct gcgtgttgcg ggtttctacc ccggtacgca gatctttaat
    45901 gcgggagtca tcaaggacat aagaacgaat ctggctgccc cagccgatgt cggatttgtt
    45961 atcttccatc gcctgtttct cggcattttt cttctgcatc tccagttcat aaagcttcgc
    46021 tttcatctgc ttcatggcct ggtctttgtt cttgtgctgg gaacggtcgt tctggcattg
    46081 ggtcacgatc ccggttggga tgtgggtaat acgcaccgca gattcggtac ggttaacgtg
    46141 ctgaccgccc gcgccggacg cgcgataaac gtcaatgcgc agatccgccg ggttgatttc
    46201 gatatcaata tcatcatcaa cttccgggta aacaaacgca gagctgaacg atgtgtggcg
    46261 acgaccgccg gagtcaaacg ggcttttacg caccaggcgg tgaacgccag tttctgtacg
    46321 cagccagccg taagcgtaat cgccggagat tttgatcgtc acggatttaa tacccgccac
    46381 ttcaccttcc gactcttcga tgatttcagt tttgaaacca cgcgattcag cccagcgcag
    46441 atacatacgc tcaagcatgc tcgcccagtc ctgtgcttcc gtaccgccag accccgcctg
    46501 aatatcgagg tagcagtcgg cgctgtcata ttcgccagag aacatacggc ggaactcaag
    46561 ctgcgccagt ttttcttcca gggcgtcgag ttcagcaacg gcttcgttaa aggtttcttc
    46621 gtcgtcagct tctacagcca gttccagcag accagaaaca tcttccagcc cctgtttcat
    46681 ttggtcgagg gtgtcgacaa cggcttcgag ggaggaacgc tctttaccca gcgcctgtgc
    46741 gcgttcgggt tcgttccaga catccggctg ttccagctcg gcgtttactt cttccagacg
    46801 ctctttcttg gcgtcgtagt caaagatacc ccctaagaac gtcggagcgt tccgtgaggt
    46861 cctgaatgcg attatttacc ggattaattt caaacatggt ctgatttctt ttattgagct
    46921 agtcaaaatg cggtgataag agcgggattg tacccaatcc acgctctttt ttatagagaa
    46981 gatgacgcta aattggccag atattgtcga tgataatttg caggctgcgg ttgccgcgaa
    47041 actcgttgat atcgagctta taagccagtt gcacttcacg cacgccgtta tccggccaga
    47101 gggcggtatc gacattaaaa gcaataccat ccagcagtgg accgccgccg accggttcga
    47161 ccatcacctt caaatgacgt tcgcccacca gccgctgttg cagcagacgg aaatgaccgt
    47221 caaacagcgg ctccgggaac atctgccccc acgggccagc atcgcgcagc aactgcgcca
    47281 cttccatggt catttcggcc gggcttaacg gaccgtctga caccacttcg ccttgcaata
    47341 gcgaagggtc cagccactct gtaaccagtt cgccaaaccg ttgttgaaag agttcgaatt
    47401 tatcctcttc cagcgacaaa cccgccgcca tcgcatgacc gccaaacttc agcatcatgc
    47461 cagggtagag tgtgtctaat cgctccagtg catcacgcat atgcagcccc tgaatggagc
    47521 gacctgaacc tttcagcgta ccatcacctg ctggcgcaaa ggcgataacc ggacggtgaa
    47581 aacgctcttt gatgcgcgaa gccagaatac cgacaacgcc ctgatgccat tcggggtggt
    47641 acattgccag cccgccgggt agcgtgtcgc gacttcgctc cagtttctcg cacagggtca
    47701 gggcttcaac ttgcattcct tgttcgatct cttttcgcgt ctggtttagc gcatcgagtt
    47761 catttgccag cacgcgcgct tcgccgatgt tgtcgcacag caacagcgcc acaccgacgg
    47821 acatatcgtc cagtcgcccg gcagcattga gacgtggccc cagcgcaaaa cctaaatcgc
    47881 tggcggcgag tttttgcgca tcgcgatttg ccacttccag cagcgcttta atccccggac
    47941 ggcactttcc ggcacggatg cgactcatcc cctgccaggt cagaatgcga ttattagcgt
    48001 ccagcggcac gacgtccgct actgtcccca gcgctaccag atccagcagt tctgccaggt
    48061 taggaattgc gatgccacgc tcatcaaacc agccctgatc gcgcaaaaag gtgcgcagcg
    48121 ccagcatcag ataaaacgcc acacccacgc ctgccagtga tttcgacggg aaattacagt
    48181 cgcgcaagtt agggttaatg atcgcttccg ccgcgggtaa tgtttcgcct ggcaaatggt
    48241 gatcggtaac aataaccgga atgcccaacg agcgagcgtg ttcaaccccc gcatgggagg
    48301 aaataccgtt atccaccgtg acaattaact gcgcgccacg ggcatgggcc tgatcgacca
    48361 cttccgggct taagccgtaa ccgtcttcga aacggtttgg taccaggtag tcgatattgc
    48421 tgcaaccaag cgagcgcatc gccagcacgc ttagagccgt gctggtcgcg ccgtcggcgt
    48481 caaaatcgcc gaccacaata atccgcgttc cttcgcgaaa agcgttgtaa aggatctcaa
    48541 cggccttttc gacgccgctc agttgctgcc agggcaacat acctttaaca ctgcgttcca
    48601 gttcttgcgc actgcgcacg ccccggctgg cgtataaacg gcgcagcaag ggaggcaatt
    48661 cagcgggcaa gtctgccgtt tcatcgactt cacggcgacg aagttgtatc tgttgtttca
    48721 cgcgaattat ttaccgctgg tcattttttg gtgttcgtcg aggaattctt tcatctcttt
    48781 cggcggctgg taacccggaa caattgtgcc attgctcagc acaactgccg gagtaccgct
    48841 aacgccaagc tggacgccaa gtgcgtaatg gtcggcaata tccacgtcgc agctggctgg
    48901 tgcgacgctt ttacctgcca tcacatcatc aaacgctttg tttttatctt tcgcacacca
    48961 gatagctttc atttctttct ctgcatcgct gtccagcccc tggcgcggga aagcaagata
    49021 acgcacggtg atccccagcg cgttatagtc tgccatttgc tcatgcagtt tgtggcagta
    49081 accacaggta atatcagtaa acacggtgat gacgtgtttt tcctgcggcg ctttataaac
    49141 gatcatctct ttttcaagcg cattcaactg ctttaacagc atcttattgg tgacattgac
    49201 cggagccgtg ccgctaacgt catacattgg cccctgaatg atatgtttac catcatcggt
    49261 gatgtacaac acgccgctgt tagtcagaac tgtcttcatg ccagctacgg gcgcgggctg
    49321 aatatcgctg cttttgatgc ccattttggc taacgtttgt tgaattgccg cgtcatcagc
    49381 ctgagcaaaa cctgaaaacg ccgctaacaa agtaaacaac ataaaacctt tcttcataaa
    49441 tcttcccgtt cttttcagac atcacgcccg cgggtgatgc tgttgatgaa gttgccgcag
    49501 acgctcggta gcgacatgcg tataaatttg cgtggtggag aggtcgctgt ggcccagtag
    49561 catctgcacc acgcgtaaat ccgcaccatg atttaataaa tgggtggcaa aagcgtgacg
    49621 caatacatgc ggtgacagct tttcgctgtc gatacccgcc agcacagcat aatgtttaat
    49681 gcggtgccag aaggtctgtc gcgtcatctg ctgcgcacgc tggctgggaa acaacacgtc
    49741 aattgacaca ccattcagca gccacggacg cccatgttcc agataggttt ccagccagta
    49801 aaccgcctct tcacctaacg gtaccaggcg ctctttgttg cctttaccaa tgacccgcac
    49861 tacgccctga cgcaggctga tatcactcat tgtcagtccg accagttcag agacacgcag
    49921 cccggtagca tacaacactt caagcatggc tttatcgcgt agctccagtg gctgatcaat
    49981 taatggtgcc tgtaataaac gttcgacctg cgcttcgctt aaatcttttg gcaaacgctg
    50041 gggcaatttc ggtgaagcga gatgcgcact gggatcgtct tcacgaaact tttcgcgata
    50101 aagatactgg aacaatcggc gcactgcact cagcaaacgc gctgagctgg tggctttata
    50161 cccgccctcc agccgttctg ccagtaatgc ctgcaaatcg tcactttgcg ccgtcgccag
    50221 cgtcaacccg cggtgatgca accactccac catcattgac agatcgcgac ggtaagcgtt
    50281 caacgtattt tcagccagat ttttttccag ccacagagca tcaagaaact gctcgatgcg
    50341 tgccagatcc tgtttcactt gcgcccctta tggtcactca tttgatccat tatgccttat
    50401 tgtgccgtga ctaaagcgat tctgatacac tagccgcaaa agccacagca gaatcgagaa
    50461 gcttacgtta tgaatatggg tcttttttac ggttccagca cctgttacac cgaaatggcg
    50521 gcagaaaaaa tccgcgatat tatcggccca gaactggtga ccttacataa cctcaaggac
    50581 gactccccga aattaatgga gcagtacgat gtgctcattc tgggtatccc gacctgggat
    50641 tttggtgaaa tccaggaaga ctgggaagcc gtctgggatc agctcgacga cctgaacctt
    50701 gaaggtaaaa ttgttgcgct gtatgggctt ggcgatcaac tgggatacgg cgagtggttc
    50761 ctcgatgcgc tcggtatgct gcatgacaaa ctctcgacca aaggcgtgaa gttcgtcggc
    50821 tactggccaa cggaaggata tgaatttacc agcccgaaac cggtgattgc tgacgggcaa
    50881 ctgttcgtgg gtctggcgct ggatgaaact aaccagtatg accttagcga cgagcgtatt
    50941 cagagctggt gcgagcaaat cctcaacgaa atggcagagc attacgcctg atctcactga
    51001 cggcttagcg catatgcttt gccgtcatct ttatctttgc gtctcttgtt gcaacaaaat
    51061 ccgccgtaaa tcccgccatt cggcttcgtc catgctgtcg gctgccagcc ataaatgttg
    51121 ccgtttaccg ccatcagaac gtaaacgcag catcatgccg ctcttaatca tccacggtgc
    51181 tttgacgatg caccactcct gcccttgcca acgcaaacgc ccgtccatca acaagcgaat
    51241 ttccccctgg cgagcattaa tacgccgctg gctgcgaacg caatcaaaca ccaccagcga
    51301 aagtaacacc atccataacg gggtgtaact gagtggccag ggcatgagta aaataacagc
    51361 ggcaaccagc ccatgaatca gcaaggaaag ccactgtgcg cgccaggaga cgcgcaaatc
    51421 agattgccac aggaccacgt tcccggttcc gtgtctggat gagtcggacc atcatttcca
    51481 gttctgcatc ggccggttta ccgtgattca tcagccagtt aaacaggtcc ggatcgtcac
    51541 attccagcag acgaataaag atgcgttttt cgtcatcgct taagctgtcg tactcatgtt
    51601 cgaaaaacgg catgattgaa atatcgagtt cgcgcatacc acggcggcat gcccaatgaa
    51661 tgcgggcttt gttgttaatg tccatcttct tcctgtctca cgaaaatcca gtacccggct
    51721 attgtaacgt gtttttcgcc ttcttttacg ggaatatcag taaacaccat cgcgatcgcg
    51781 aaattaattc acaacaattc aatggcttca tttttttgga agtcgcctcg cagaaggcac
    51841 agatcgcgta gtgaaagcac ttgcattgcc tcatagctct tttaccatta gtcattaata
    51901 cgccgttaag caactcagga ctctattatg gcttttacac cttttcctcc ccgtcagcct
    51961 acggcttctg cccgtttacc gctgacgctg atgacgcttg atgactgggc gcttgccacc
    52021 attactggcg cggacagcga aaaatatatg cagggtcagg tgacagcaga tgtcagccag
    52081 atggcagaag atcagcacct gctcgccgcc cattgcgacg ccaaaggtaa aatgtggagc
    52141 aatttacgtc tgttccgcga cggcgatggc tttgcatgga ttgaacggcg cagcgtgcgt
    52201 gaaccgcagc tgactgaact gaaaaaatat gcggtattct ctaaagtgac catcgcgcca
    52261 gacgacgagc gtgtgctgct tggtgttgcc ggttttcagg cgcgcgccgc gctggcaaat
    52321 ctctttagcg aactgccttc gaaagaaaaa caggtagtca aagaaggcgc gaccactttg
    52381 ctatggtttg aacacccggc agaacgtttc ctgatcgtaa ccgatgaagc tactgctaat
    52441 atgctgaccg ataaactgcg cggtgaagcg gaactgaaca atagccaaca gtggctggca
    52501 ttaaacattg aagcgggttt cccggtgatt gatgccgcca acagcgggca gtttatccca
    52561 caggcgacca atctccaggc gctgggcggt atcagcttta agaaaggctg ttataccgga
    52621 caagagatgg tggcgcgggc caaattccgc ggtgccaaca aacgtgcgct ctggttactg
    52681 gcaggtagcg ccagccgact gccagaagca ggtgaagact tagagctgaa aatgggcgaa
    52741 aactggcgtc gtaccggtac ggtgctggct gcggtcaaac tggaagatgg tcaggtcgtg
    52801 gtgcaggtgg tgatgaataa cgatatggaa ccggacagca tcttccgcgt gcgcgacgat
    52861 gcaaatacac tgcatatcga gccgctgccg tattcgctcg aagagtaaat ttctatatcg
    52921 cttcgggcat ctattgcctg aagcgacact gatgtgtctt ttcatgcaga caaaggcaca
    52981 gcaattacgc ctgcccaata tacaaataga tcgccagaaa gtggcacaca ctaccgccga
    53041 gtacaaagcc gtgccagatg gcatgattgt acggaatgcg tttgcagaca tagaaaatca
    53101 ccccgagcga ataaaccacg ccgcctaccg ccagtaaggt aacgctgcct gccgcgagct
    53161 taactgccat ttcataaatt accaccagcg acaaccagcc catcgccaga taagtcacca
    53221 gagataaaat tttaaatcgg tgcgcgatgg tcagtttgaa cagaatcccc agcaatgcca
    53281 ggctccagat aacaatcatc aatccgcgcg ctaacggaga atctaacccc accagcaaaa
    53341 acggtgtata ggttccggca atcaacaggt aaatggcgca atggtcaaat ttcttcagcc
    53401 acatttttgc ccgttgatgg ggaatggcgt gatagagcgt cgaagcgagg aacagcagga
    53461 tcatactgcc gccatatagg ctataactgg ttatcgccgt ggcgctggca ttgagatcca
    53521 ccgcctgaac cagtagcaac accagcccaa cgataccaaa caccagccca atgccgtgac
    53581 tgacgctgtt ggcaatttcc tctgccagcg aatatccctg cttaatgagg ggcttctgaa
    53641 ccataactta ctccggagaa acgtacacgc acatgtatac ctctccagcg taactgagaa
    53701 tagttccagt gaacacctgt tagctaaaat aaattctgat tttggactaa tctccgaaaa
    53761 atcatgaaat taaatgcgaa atttcaacta acaggcgtga gttcaattta aaggcattta
    53821 aattcaatca cataaaactg tgtctggccg ggatagatgt cggcaatgac ctttttcagt
    53881 tcaggcaggg tcatattttc ctgctctgca tgtttttccg tcagcgtctc cagcgttatg
    53941 gttgaggttg cggtgacttc gatggtgcaa aaatagccat catcttcaaa gcgcccaacg
    54001 cgcagcacat cgcccgtttt aaagtgcgat tcagattcgt cgcggatggt gatggtttta
    54061 cgtccagcca gaatgtcatc ctggaaacgt tgaaaaaaag tgatgtcgtt tggctgcatg
    54121 gtactatttc ctgtaagaat tgattcatct ggagcctatg atagtgaaaa aactcacctt
    54181 accgaaagat ttcttatggg gcggcgcagt tgccgctcat caggtcgaag gcggctggaa
    54241 caaaggcggc aaagggccga gcatttgtga cgttctgacc ggtggcgcac acggcgtgcc
    54301 gcgcgaaatc accaaagaag tcttgccagg caaatactat ccaaaccatg aagccgttga
    54361 tttttatggt cactacaagg aagacatcaa gctatttgcc gaaatgggct tcaaatgttt
    54421 tcggacttcc atcgcctgga cgcgtatttt tccaaaaggt gatgaagctc agccaaacga
    54481 agaagggctg aagttctacg atgatatgtt cgatgaactg ctgaaataca acatcgaacc
    54541 ggtgatcacc ctctcccact ttgaaatgcc gctgcatctg gtgcagcaat acggtagctg
    54601 gaccaaccgt aaagtggttg atttctttgt ccgtttcgcg gaagtggtgt ttgaacgcta
    54661 taagcataaa gtcaaatact ggatgacctt caacgaaatt aacaaccagc gtaactggcg
    54721 tgcaccgctg ttcggttact gctgctccgg cgtggtttat accgagcatg agaacccgga
    54781 agagacgatg tatcaggtgc tgcatcacca gtttgtcgcc agcgccctgg cggtgaaagc
    54841 tgcgcgtcgc attaacccgg agatgaaagt cggctgtatg ctggcgatgg tgccgctcta
    54901 tccctactgc tgtaacccgg acgatgtgat gttcgctcag gagtcgatgc gcgaacgcta
    54961 cgtctttacc gatgtgcagc tacgcggcta ttacccgtcc tatgtgttga acgagtggga
    55021 gcgtcgcgga tttaacatca aaatggaaga cggcgatctc gatgtactgc gtgaaggcac
    55081 ctgcgattat cttggtttca gctattacat gaccaacgca gtgaaggccg aaggcggcac
    55141 cggcgatgcg atctctggtt ttgaaggcag cgtaccgaac ccgtatgtta aagcgtctga
    55201 ctggggctgg cagattgatc cagtaggtct gcgctatgcc ctttgcgaac tgtatgagcg
    55261 ttatcagagg ccgctgttta ttgtcgaaaa cggttttggc gcttacgaca aagtggaaga
    55321 agatggcagc atcaacgacg actaccgcat tgactacctg cgcgcccata ttgaagagat
    55381 gaaaaaagca gtgacttacg atggtgtgga tctgatgggc tacacaccgt ggggctgcat
    55441 cgactgcgta tcgttcacca ccgggcagta cagcaaacgc tacggcttta tctatgtgaa
    55501 taaacatgac gacggtactg gcgatatgtc gcgttcacgt aagaagagct ttaactggta
    55561 caaagaggtg attgccagca acggcgagaa gctttaagtc gatgaagtac cggatgcaat
    55621 acttgttgca tccggtcaga caacttccct gttttatttc ccgcccgcca aatcgataaa
    55681 acttcccgtg acgtaagagg ctttatcact tagtagccag acaatggcct gcgcgacctc
    55741 ttctgcctgt ccaccacgct gcatggggat gttcgactta acgcgatcga cgcgtccagg
    55801 ctcgccgccg ctggcgtgca tttcggtata aataaaccct ggccgcacgc agttaacccg
    55861 gatcccctgc gcggcgactt ccagcgatag tccggtggtt aacgtatcaa tcgccccttt
    55921 cgatgccgcg taatcaacat attcccctgg cgaacccaac cgtgaggcca ccgaagagac
    55981 attgacgata gcgccgccac tgccaccatt tttaagcgcc atgcgtttta ccgcctcgcg
    56041 gcagcagaga aaatatcccg tcacgttggt ggaaagtact cggttgattc gctctgcggt
    56101 aaggttttca acggtgcact gggtaaacaa gatcccggcg ttattgacca gcgctgctag
    56161 cggttcatcg tgctgatcga ttgctgtaaa catcgcaacg acctg
//
LOCUS       DAFATA010000019        54172 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFATA010000019 DAFATA010000000
VERSION     DAFATA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.019"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(48..212)
                     /locus_tag="LGJ20_001535"
     CDS             complement(48..212)
                     /locus_tag="LGJ20_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385614.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(235..936)
                     /locus_tag="LGJ20_001536"
     CDS             complement(235..936)
                     /locus_tag="LGJ20_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBK2385615.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHELTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1041..1937
                     /gene="yhaJ"
                     /locus_tag="LGJ20_001537"
     CDS             1041..1937
                     /gene="yhaJ"
                     /locus_tag="LGJ20_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBK2385616.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(1988..2344)
                     /locus_tag="LGJ20_001538"
     CDS             complement(1988..2344)
                     /locus_tag="LGJ20_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBK2385617.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(2586..2951)
                     /locus_tag="LGJ20_001539"
     CDS             complement(2586..2951)
                     /locus_tag="LGJ20_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBK2385618.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(3244..4230)
                     /gene="yqjG"
                     /locus_tag="LGJ20_001540"
     CDS             complement(3244..4230)
                     /gene="yqjG"
                     /locus_tag="LGJ20_001540"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBK2385619.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(4300..4692)
                     /locus_tag="LGJ20_001541"
     CDS             complement(4300..4692)
                     /locus_tag="LGJ20_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBK2385620.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(4878..5177)
                     /locus_tag="LGJ20_001542"
     CDS             complement(4878..5177)
                     /locus_tag="LGJ20_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBK2385621.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(5167..5571)
                     /locus_tag="LGJ20_001543"
     CDS             complement(5167..5571)
                     /locus_tag="LGJ20_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK2385622.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(5574..5879)
                     /locus_tag="LGJ20_001544"
     CDS             complement(5574..5879)
                     /locus_tag="LGJ20_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBK2385623.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(5917..6285)
                     /locus_tag="LGJ20_001545"
     CDS             complement(5917..6285)
                     /locus_tag="LGJ20_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBK2385624.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(6432..6815)
                     /gene="mzrA"
                     /locus_tag="LGJ20_001546"
     CDS             complement(6432..6815)
                     /gene="mzrA"
                     /locus_tag="LGJ20_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBK2385625.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(6819..7481)
                     /locus_tag="LGJ20_001547"
     CDS             complement(6819..7481)
                     /locus_tag="LGJ20_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2385626.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(7826..8602)
                     /gene="exuR"
                     /locus_tag="LGJ20_001548"
     CDS             complement(7826..8602)
                     /gene="exuR"
                     /locus_tag="LGJ20_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBK2385627.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            8898..9614
                     /locus_tag="LGJ20_001549"
     CDS             8898..9614
                     /locus_tag="LGJ20_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBK2385628.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            9644..10168
                     /locus_tag="LGJ20_001550"
     CDS             9644..10168
                     /locus_tag="LGJ20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015366620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2385629.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVAF
                     ICLRISNPDNRINT"
     gene            10235..12919
                     /locus_tag="LGJ20_001551"
     CDS             10235..12919
                     /locus_tag="LGJ20_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBK2385630.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            12916..14004
                     /locus_tag="LGJ20_001552"
     CDS             12916..14004
                     /locus_tag="LGJ20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBK2385631.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(14092..15390)
                     /gene="exuT"
                     /locus_tag="LGJ20_001553"
     CDS             complement(14092..15390)
                     /gene="exuT"
                     /locus_tag="LGJ20_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBK2385632.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            15873..17285
                     /gene="uxaC"
                     /locus_tag="LGJ20_001554"
     CDS             15873..17285
                     /gene="uxaC"
                     /locus_tag="LGJ20_001554"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK2385633.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            17300..18787
                     /gene="uxaA"
                     /locus_tag="LGJ20_001555"
     CDS             17300..18787
                     /gene="uxaA"
                     /locus_tag="LGJ20_001555"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK2385634.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            18870..19421
                     /locus_tag="LGJ20_001556"
     CDS             18870..19421
                     /locus_tag="LGJ20_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBK2385635.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(19426..20670)
                     /gene="sstT"
                     /locus_tag="LGJ20_001557"
     CDS             complement(19426..20670)
                     /gene="sstT"
                     /locus_tag="LGJ20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK2385636.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(21286..22251)
                     /gene="alx"
                     /locus_tag="LGJ20_001558"
     CDS             complement(21286..22251)
                     /gene="alx"
                     /locus_tag="LGJ20_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBK2385637.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(22534..23520)
                     /locus_tag="LGJ20_001559"
     CDS             complement(22534..23520)
                     /locus_tag="LGJ20_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK2385638.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(23599..24282)
                     /locus_tag="LGJ20_001560"
     CDS             complement(23599..24282)
                     /locus_tag="LGJ20_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBK2385639.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(24359..24862)
                     /locus_tag="LGJ20_001561"
     CDS             complement(24359..24862)
                     /locus_tag="LGJ20_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBK2385640.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            24947..26083
                     /gene="rlmG"
                     /locus_tag="LGJ20_001562"
     CDS             24947..26083
                     /gene="rlmG"
                     /locus_tag="LGJ20_001562"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBK2385641.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            26274..26357
                     /locus_tag="LGJ20_001563"
                     /pseudo
     CDS             26274..26357
                     /locus_tag="LGJ20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            26354..26770
                     /gene="higA"
                     /locus_tag="LGJ20_001564"
     CDS             26354..26770
                     /gene="higA"
                     /locus_tag="LGJ20_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBK2385642.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(26815..28833)
                     /gene="fadH"
                     /locus_tag="LGJ20_001565"
     CDS             complement(26815..28833)
                     /gene="fadH"
                     /locus_tag="LGJ20_001565"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBK2385643.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(29159..31510)
                     /gene="ygjK"
                     /locus_tag="LGJ20_001566"
     CDS             complement(29159..31510)
                     /gene="ygjK"
                     /locus_tag="LGJ20_001566"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK2385644.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(31527..32597)
                     /gene="ygjJ"
                     /locus_tag="LGJ20_001567"
     CDS             complement(31527..32597)
                     /gene="ygjJ"
                     /locus_tag="LGJ20_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HBK2385645.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYDSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(32731..34164)
                     /locus_tag="LGJ20_001568"
     CDS             complement(32731..34164)
                     /locus_tag="LGJ20_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2385646.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(34227..34676)
                     /locus_tag="LGJ20_001569"
     CDS             complement(34227..34676)
                     /locus_tag="LGJ20_001569"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBK2385647.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(34673..37765)
                     /gene="ebgA"
                     /locus_tag="LGJ20_001570"
     CDS             complement(34673..37765)
                     /gene="ebgA"
                     /locus_tag="LGJ20_001570"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBK2385648.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(37949..38932)
                     /gene="ebgR"
                     /locus_tag="LGJ20_001571"
     CDS             complement(37949..38932)
                     /gene="ebgR"
                     /locus_tag="LGJ20_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBK2385649.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFICGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            39151..39483
                     /locus_tag="LGJ20_001572"
     CDS             39151..39483
                     /locus_tag="LGJ20_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBK2385650.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(39525..40904)
                     /gene="ygjG"
                     /locus_tag="LGJ20_001573"
     CDS             complement(39525..40904)
                     /gene="ygjG"
                     /locus_tag="LGJ20_001573"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBK2385651.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            41322..42842
                     /gene="aer"
                     /locus_tag="LGJ20_001574"
     CDS             41322..42842
                     /gene="aer"
                     /locus_tag="LGJ20_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBK2385652.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(42996..43619)
                     /gene="nfeR"
                     /locus_tag="LGJ20_001575"
                     /gene_synonym="yqjI"
     CDS             complement(42996..43619)
                     /gene="nfeR"
                     /locus_tag="LGJ20_001575"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBK2385653.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            43907..44671
                     /gene="nfeF"
                     /locus_tag="LGJ20_001576"
     CDS             43907..44671
                     /gene="nfeF"
                     /locus_tag="LGJ20_001576"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBK2385654.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(44725..44800)
                     /locus_tag="LGJ20_001577"
     tRNA            complement(44725..44800)
                     /locus_tag="LGJ20_001577"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44765..44767),aa:Ile,seq:cat)
     gene            44925..45431
                     /gene="mug"
                     /locus_tag="LGJ20_001578"
     CDS             44925..45431
                     /gene="mug"
                     /locus_tag="LGJ20_001578"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBK2385655.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(45510..47351)
                     /gene="rpoD"
                     /locus_tag="LGJ20_001579"
     CDS             complement(45510..47351)
                     /gene="rpoD"
                     /locus_tag="LGJ20_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK2385656.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(47546..49291)
                     /gene="dnaG"
                     /locus_tag="LGJ20_001580"
     CDS             complement(47546..49291)
                     /gene="dnaG"
                     /locus_tag="LGJ20_001580"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK2385657.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(49402..49617)
                     /gene="rpsU"
                     /locus_tag="LGJ20_001581"
     CDS             complement(49402..49617)
                     /gene="rpsU"
                     /locus_tag="LGJ20_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK2385658.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            49854..50867
                     /gene="tsaD"
                     /locus_tag="LGJ20_001582"
     CDS             49854..50867
                     /gene="tsaD"
                     /locus_tag="LGJ20_001582"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK2385659.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(50910..52373)
                     /gene="ttdT"
                     /locus_tag="LGJ20_001583"
     CDS             complement(50910..52373)
                     /gene="ttdT"
                     /locus_tag="LGJ20_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBK2385660.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(52421..53026)
                     /gene="ttdB"
                     /locus_tag="LGJ20_001584"
     CDS             complement(52421..53026)
                     /gene="ttdB"
                     /locus_tag="LGJ20_001584"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBK2385661.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(53023..53934)
                     /gene="ttdA"
                     /locus_tag="LGJ20_001585"
     CDS             complement(53023..53934)
                     /gene="ttdA"
                     /locus_tag="LGJ20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBK2385662.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
BASE COUNT        13244 a        13809 c        14082 g        13037 t
ORIGIN      
        1 ttttgtaggc cggataaggc gttttcgccg catccggcaa cctgtcttta cgcggtagct
       61 tcatcctcgg ctaaacgcgt ttcagcatcc gcgacgatgg cttccagctc gctcaacatc
      121 tcttcatagc caaaattttt ggcggtgcga gcaggttctt tcgccaccac gggcttcact
      181 ggctggcgct ttttggccga ttttttactc atcatcatct ccctgtaaaa acaactagac
      241 aggcaaatct atcagcaaag cgcgcagtgg ggaatcggca accagcgtaa tgttagcctc
      301 gtcacgaata aacgccccat caccgcaggt cagcgccgct ttctcttcat gatgcgtaag
      361 ctcatgaaat ttcccgtgaa tcgattgcaa atacgcgcgt ggcccatgca actggaaatt
      421 cgcactttcg cctttgtcga gcacgatatg gtgcagccac acttgctggc gtaattgcag
      481 gcttcccatc gtaccctctg gcgaggcgat taattgctgc ttgcccatat taagcgccag
      541 tttttgaatc agcggattct ctcgctgcgg gcaggcgtcc agccaaagct gcattcgtgt
      601 taacggtttg tctttgctga gattgtgttc gctgtagctg acacccggct gggtagagag
      661 cagcaacgcc tcaccggcgc tggcctgaac atgattgcct tcgctgtcgc gatactctgc
      721 ttctccatcc agaatcacat ttaaaatatc gactttggga taggttcgcg gctgaaaggc
      781 ggcacctggg gccagcactt cctggttaag cacacgcagg gaggcatagc ctaacaattt
      841 cgggtcgaag tagtgtccaa aggaaaaagt ataccgggcc tgcaaccatc cgtagtctgc
      901 ttgtccacac tgcctggcag ttcgggtagt aatcataatt cttgacctct ctttacttcc
      961 ttttatggta aggggctgga cgctacattg ttagccagat attctgcccg gtatgttcaa
     1021 atttcctgaa tgagaacgaa atggccaaag aaagggcatt aacgctggaa gcactacggg
     1081 ttatggatgc gatcgatcgc cggggcagtt ttgcggcggc ggcggatgag ctgggacgcg
     1141 tgccttccgc acttagctac accatgcaaa aactggaaga agagctggat gtggtgctgt
     1201 ttgaccgctc gggccatcgc accaaattca ccaatgtcgg gaggatgttg ctggagcgag
     1261 ggcgcgtttt gctggaagcc gcagataaac tgactaccga tgcggaagcc ctcgcgcgcg
     1321 gttgggaaac gcatctcacc attgtgaccg aagcgctggt accgacacct gcctttttcc
     1381 cgttaatcga caaactggcg gcaaaagcca atacccaact ggcaatcatc accgaagtgc
     1441 tggcgggggc gtgggaacga ctggagcagg ggcgggcgga tattgttatc gcgccggata
     1501 tgcattttcg ttcctcgtcg gagatcaact cgcgcaagct ctatacgtta atgaacgtct
     1561 acgttgccgc gcctgatcac ccgattcatc aggagccgga accgttatct gaagtgacgc
     1621 gcgtgaaata tcgtggaatt gcggtagcgg ataccgctcg tgagcgcccg gtgttgaccg
     1681 tacagctgct ggacaaacag ccgcgcttaa cggtgagcac gattgaagat aaacgtcagg
     1741 cattactggc ggggcttggc gtggcgacga tgccgtatcc gatggtcgaa aaagatattg
     1801 cggaagggcg gttgcgtgtc gtcagcccgg aatcgaccag cgagatcgat attattatgg
     1861 cctggcgtcg tgacagtatg ggggaagcga aatcctggtg tctgcgggaa attcccaaac
     1921 tttttagcgg aaaataatat ttgttattat taataaaaag gggcgaaatg tcgccccaat
     1981 tctaagatta atttgaacca aacttaggat cgtttccgta acgattgctg ccagaattgc
     2041 cttctaaaca ggcaaataca aacagaacta accagccaat gatcggtacc agatagagaa
     2101 gcgcccacgc acctgaacgg tcagtatcgt gcagacgacg aacacacaga gcaatgaccg
     2161 gaatgattgt tgctgccagg taaatgagag aaaggaatgg aaactctaaa cctaaaatca
     2221 attgaatgac attgataata gcgccgacga tggcgttaat cagagtaaac atccagtact
     2281 ctttacgacg tgcgcgacca gagaaaccaa cataattttt taatacggct aaataccact
     2341 gcataaaaac atccctttaa ttaaaagtga cgaaagtcta ttccgggtat tgtgtaaatg
     2401 ctattcgtat ctttcttttt cggatgttat aaaggttttg atatttaaga taaatacatg
     2461 gttgtttttt ataggtaatt tatataacga cattgaaata ttttaaataa acattagtaa
     2521 attattcatt catcaacatg atgataaaag ccggatgagt cagacatccg gcgagcagat
     2581 ttgttttaag gttccagttt cggatctggt ccaaagcggt tttcgcctgg cgtacccgcc
     2641 tggcagttaa agacgatgat aataagccag ccgataaacg ggattaagaa caacagtgcc
     2701 caccacgccg agcggtcggt gtcgtgcagg cggcggaact gaactgccca ccacggtaaa
     2761 aacaccagaa taccgtagat tgtcgtcagg atgccttcgc caccagcgcg ttgccagcct
     2821 aacattttat ccagcagccc cagcacgaac gtaaagatga tgttgaccag aataaacatc
     2881 cagtactctt tgcgccgcgc acgaccccgg aaaccgacat aattttttag tactttcaga
     2941 taccagtcca tttttgcctc aattttgctc aatcacttgt ttttaaagca gcaaattaag
     3001 aatagacgtg aatgaaatgg cagtaaatat tcgttcaagg aattaataaa gtgctgtgaa
     3061 ggcgcgctaa gactgatgac aagcgcaaaa tcgccagatg cgctacgctt atctggcctg
     3121 cgcggtcatt gcaacatatt gaatataaat gttttgtagg tttgataagg cgttcacgcc
     3181 gcatccgaca tgaacaacat gcacttagct aattatcaga ggcacctcgt aaggtgcctt
     3241 tttttaaccg aagcgaacat ctcgtccatg cggttcatcg agatcctgcc atggaccaat
     3301 tgaaataatc cccgtagggt tgatggtctt atggctgcgg aagtaatgat tacggatgtg
     3361 atcgaaattg actgtttcgg caattcccgg catctggtag atatcacgca ggaagccata
     3421 cagattaagg taatcgctga tgcggtgctt atcacacttg aagtgggtca catacactgg
     3481 atcaaaacgg accagcgtgg tccacaggcg aatatcggct tcggttagct ggttgccggt
     3541 caggtaacga tgctggccta aaatctgctc cagtcgcgcc agcgattcaa acactttcgc
     3601 caccgcctcg tcgtaggctt gctggctggt ggcaaaacca gctttataca cgccgttgtt
     3661 aacggtgtca taaatccagc cgttaagttc gtcaattttc gtttgcaggg ctggtgggta
     3721 gtaatcaccc gcttttgcgc ccagcgcatc aaacgcggta ttgaacatgc ggatgatttc
     3781 tgctgattcg ttgctgacga tggtgtggtt ctttttgtcc cacagcacgg gaacagtaac
     3841 tcgtccgctg tagtgtggat cggcgtggag ataaagctga tacagaaatt catgttgata
     3901 gagcgtatct ccggttgctc ccggaaaact gtcatcaaag gtccagccgt tttccagcat
     3961 cagcgggttc actacggaaa cggaaataaa cggttccagt cctttgagtt tgcgcatgat
     4021 cagcgtgcgg tgcgcccacg ggcaggcgag tgaaatatag agatgataac gatctttctc
     4081 tgcgataaaa ccgcctgtgc cagtggggcc aggtgcgcca tcggcagtga gccagttacg
     4141 aaatgcggaa gctgaacgtt gaaatttacc gccggtagat ttggtatcgt accaggtgtc
     4201 atgccagacg ccgtcaatca gttgacccat ttttctctcc tccaatagca aaaacgagga
     4261 gcttcctcct cgttttttat cgttaagtat agcgcgtgct taccactttt tattcagcag
     4321 gcggtcgatg ctatacgcgc ccggaccggt aattgccagc agcaggaatc cgccagaaat
     4381 tgtcaggttt ttcatgaaca tcagcgagtt gacgccttca gcaaagttgc tgtgaaataa
     4441 aaatgccgtc agcagcgtaa agcccgcagt aaacagggct gtggtgcgag tcaggaaacc
     4501 gaacaggatt gccagaccac caccgaactc aagcagaatc accagcggca gcataaaacc
     4561 cgggacaccc attgcttcca tatattgttg ggtaccagag taagcggtga tttttcccca
     4621 gcctgcggta ataaacagaa tcggcattaa aatgcgcgct accagtacac caacatcttc
     4681 taattttttc atcatactct ccactgcgcc actcaggctg ctgattgttt ttttgtgcaa
     4741 gggcgcggta ttagcgtcgt tgctgtcgat ggagagaatc ataaacgtgg tgaatgatga
     4801 ttgttagcaa ggaaaactgt caaaaatctt caaaaaattt gagggataag gccggaattg
     4861 ctccggccag agggaagtta accgcgaagc tgttgctgct tgagggtcgt tttaaccaga
     4921 cgccaggcgc tccatacgcc aaaaccgcgt ctggcccagc ggaccagcat attaggatgg
     4981 cgaatcgtcc agatcgccat cacgctactg ccaaccagcg cccaggagcg cagacttagc
     5041 agcatattcc agcgacgatc gtaagcgcct gttgcctcca gccattcacg acgactggcg
     5101 gaaagatcca gccgttgttg ctggatctgg ctaagcagtt gcgccttacg tcgttcacgt
     5161 tcgactttac tgctcacggg actcctcctc gagcagttgc cgatcgtttg ccagctcatg
     5221 gcgtgtatgg cgcagcaagg tggatttacg cgatttacgt aacgtccaga taccgccaat
     5281 cagtgccagt agcagcaaca ccacggtggt ggcaatcatc gcattcaggc gatattgcgg
     5341 atcaaccgcc caaataatta gcaccatcag gctcataaga ccaaatgcag cgaaaagcat
     5401 cgtcaggccc agcatcagta aaagttgaaa gagattagct ttttcctctt ccagctccac
     5461 caccgccagc cgcagacgtg tttccaccat ttcaaccatg atggaaacaa ttcgctgccc
     5521 gatgcccaga acgcttttac cgggcccttg cgcgtgatga gtgtccgcca taattaacga
     5581 cgcgacagca gaacgccgag cactacaccg attgcagcgc caattcccac gccggtccac
     5641 ggattttcac gcacatactc atcagcacgc gccgccgcga cacgggtttg tttggcgatg
     5701 gcatcaccgg tttcacccag gcgataacgg ctctgtttca gtgcctgctc cgctttgcta
     5761 cgaatcttac tcaactcttc tttcgacttc tcgccagatg agctaagcac ctcttccagc
     5821 gtatcggaaa gggatttcaa ctcagcacgc agatgttccg tagtgtgttc tttcgacata
     5881 gttttctcca ggtgagtaag tagtgactat tgtgagttag tagtcgcgcg cttccagctt
     5941 tttcagctct tcctgcgctt ctgccagttt ccgttcgcgt ttggcaatct tatcggcatc
     6001 gcctttttgc ttcgcctcgg ctaaatcttg ctggcgttcc gccacctcat ctttctgctt
     6061 tgcgattttc ttgtgatgat cggcacgcag ctggctatct gaacagttgg cccggacttc
     6121 actcagggct ttattcagac cgtcaatacg attctggttt tggtgttttt cggcatagct
     6181 gatctcctta aggatatttt gctccttttc ctgacacagg gtagtggcat aactaccggc
     6241 actaagagca aagagagaaa cagctaaagc gatgcggtat ttcattcttg aaccttccat
     6301 tgtgattcgg acctattaca aaggaacgat tgtccagaca acgaagcggt agtgttaacc
     6361 accgcctcat taatcataga cagttacggt gaaatcacca aagtgagtga tatgtttcgg
     6421 ataacaggaa gttatccgaa gcgatgagag ttatcccgta accgggtcag ccactgcata
     6481 gcctgactgt tattgtcctg ctgcgcaatg atgtagccat ggggcaatgt tctgtcgagg
     6541 accgcttttg cggcggcgct ctggtcgctg gagtcaaatg taattaacag agtgtcgttt
     6601 ttgggggtga tacttttgaa aggaatgcca tgagcgtcaa ggtgatgcca gatcgaaaaa
     6661 ccgtctggca tcgttgtgcc ttgatgaacg gcacgaatcg ccagcgtaga ctcttgctgg
     6721 cgaaccgcgg accaggccag caacagcgtg ccgaccagaa gtaaaacagt gccggaccag
     6781 gctagctggc gaagcgacat gcgaggtatt tgcatccctt acccccgatt tccatatttc
     6841 tttttccata acacgaccag agaacctgcc aggccaaaca ccagcagcac caccgggagc
     6901 agcatcaggc atgacatcag ctggtcctcg tactttaaaa ataccggcgt tttgccgagc
     6961 atgtaaccca gagttgtcag gatcaatacc cacagcagac cgctcatcca gttgaaaaac
     7021 tgaaagcgcg cgttattcag ccctgataac ccggcaatcg tcggcagcag tgttctgaca
     7081 aacgcaataa agcgaccaat taacagcgcc gataaaccgt gtttatgaaa aagatggtgt
     7141 gcgcgttgat gataatgcgc gggtaaatga gatagccagt tttgtacggt gcgggtattg
     7201 cccagccatc gcccctgaat atagctgacc cagcagccga ggctggcggc aacggtcagc
     7261 agcagaatcg tttgcgggta acccatcgcg cctttcgcaa tcaacacgcc gaccaatacc
     7321 agtaaactgt cgcccggtaa aaaggccgct ggaagcaagc cgttttcaag gaacaaaatt
     7381 acaaacaaga caaaatacaa catgccaatc atcgatggat tggccagggt ttcaaaatcc
     7441 tgcgcccaca gggcttgcag caattgggtc aaaagttcca ttcattattc ctggtgaatc
     7501 ggttaacgcc acgctggttt taaaagatgc actaccgcaa tgttattgct gttttggtat
     7561 gttctgcggg tttgatacat caacacagcc tgaactattc cctgttaaat cgtgagataa
     7621 gaaagcattc aaaaacataa attcaaattg aatctaattg taacaaaggt caacgttgtt
     7681 cgttatcaaa attacatgtc cgtggagtct gattttgctg aatgcagggg agggtggcga
     7741 gaggatttac acaggctgac actttatact tttcgcttac cgacgagaca aacaggcgcg
     7801 tcgcagcgcc tgtcggacag gagtttcatt tactgccact ggtggccgta tcgagatgca
     7861 ctaccgggtt ttcggcgaac agatagcggt cggcattgaa ctcgaagtcg tcgctggttt
     7921 cgttgaataa catgatcttg gtgttttcca ggtgctgcca cattgccagc ttagcagcat
     7981 gaggatcttt gcgaatcagc gccttgagga tttgatcgtg gtcatcacac cagttatcga
     8041 cggtacggga atcaatgtgt tcgtgcagtt ttttccagta cgggttatgg ctacgttggg
     8101 tccacatttt ttcaacgatg gccgccaggg cggagttctg cgtcgccagg gctacctgaa
     8161 tgtggaactg taaatcccac tcggaatcac ggaagcattg ttcgccgcgc gcctgttcct
     8221 gaatggccat cagtttcatg atgtcttgtt tcgttacctg agttgccgcg aactcggcaa
     8281 tattactttc gatgagctgg cgagcctgaa gcaactcaaa cggaccgtaa ttggcgaatt
     8341 ccatattatt gtcagccgcc tgctgatggc gcggctggtt ggaaaccaca tgaatacctg
     8401 aacctttacg cacttcgaca tagccttcaa cttccagcat gatgatggct tcacgaacta
     8461 ccgtacggct gacgttcttt tcatcggcaa taaagcgttc tgcaggcagt ttatcaccta
     8521 ccagatagac gccctgttcg atgcgctctt tcaggtcagc ggcaagttgt tgatacaaac
     8581 ggcgtggttc agtgatttcc atatgcgtct cccggagtgg aatgcggacg aagttatcat
     8641 accactttat tcatgggtat gccattggtc atatataaat ttggtgattg aattaatgta
     8701 ttgaattaat attgtgaata tttataaatt cctaaaaaat aagtatcgtc attgtgaaag
     8761 ctactaaatt tcagtaagtt aattcccagc ctttaaattt aatattaact aaaaatgctt
     8821 ttttatattg acttacaata aatctacatc tctaatacac caactttttg gaaacaatat
     8881 tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt
     8941 tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt
     9001 ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt
     9061 acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg
     9121 agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc gctttaccag
     9181 ccggtagtag caaaaccgta cgttttgttg cgatggaacc agagcaaaaa gagaaaaatt
     9241 atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc
     9301 tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc
     9361 aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaatcaac
     9421 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
     9481 agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa tcttgaaagc atatctggct
     9541 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
     9601 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
     9661 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
     9721 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
     9781 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
     9841 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
     9901 cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt gttccgctga cagtaacctg
     9961 gggcggtgaa gaaattaaag ctgatgcggc aaccactttt accgcaacca aaatctttgc
    10021 ttccgatgcg ttaactaacg gttctctggc taaaaacctg atgtttgctc aaaccaccaa
    10081 aggtgttctg gagacaggca tctaccgtgg tgtagtagca tttatctgtc tcaggatatc
    10141 taatccagat aacagaatta acacgtaaca aagggagggg tagccctccc ttattttaac
    10201 tgttttagta tttgttatgg atttcaggat tgccatggat aaaaaattac tggctctttt
    10261 gatcctggcg agtctcagcc cggcagaggc gacattaacc aaaattcccg cagggtttga
    10321 ggttattgct cagggacagc aggagtatat cgaggtttat ttttcaggga aaagtctcgg
    10381 taaatattat gcaatggtta atcttgatac cgtaacattt cttgatccag caagtctata
    10441 taacaagctg gaactggatg tagacgatca gaaaatcgcg catatagtga aagaaaaatt
    10501 atcgcagccg ctagctcgcc acggtgaatt ggcttgcggt tatgtacgta ctgactcagg
    10561 gtgtggtttt ctgaataccg atacgctgga aataatctat aatgatgaag aaagttcggc
    10621 aacgttgttt attaatccgc aatggaattc agctttcgat gcgaagtcat tatatttaaa
    10681 tccagacaaa aatactgtta atgcttttat acatcagcaa gacatcaatg ttctggcaca
    10741 ggatgattac caatcgttgt ctattcaggg aaacggtgcg ctgggaataa cagaaaatag
    10801 ttatattggt gcacactgga atttcaacgg ttatgatgca gatgatgtca gtgacagtaa
    10861 tgctgacgtc agcgatctct attatcgtta tgatttttta cgtcgttatt atgtgcaggc
    10921 gggtcgcatg gacaaccgca cactatttaa tgcacaaggc gggaacttta cctttaactt
    10981 tctgccactc ggtgcaattg atgggatgcg tatcgggtcg acactgagct atttaaacca
    11041 ggcgcaaagc cagcaaggaa ccccagtaat ggttctgctt tcgcgcaatt ctcgtgttga
    11101 cgcttatcgt aatgagcaac ttttgggatc gttttatctc aatagtggtt cgcaatttat
    11161 tgataccagt tcctttccgc ctggcagcta cagcgtggca ttaaaagtct acgaaaataa
    11221 ccaactcacc cgcaccgagc ttgtgccgtt taccaaaacc ggcggtctga ctgacggaaa
    11281 tgcgcaatgg ttcttacagg caggtaaaac tacatcacag gtttctgatg atgaaagttc
    11341 agcttatcag ctaggagtac gcctgccatt acatccgcaa tatgagctct acgcagggct
    11401 ggcgaatgcc gatgatgtga gtgctttcga gttaggtaat aactggacgg cagatttagg
    11461 cggggtgggg aatcttgcaa tcagcgccag cgtgttccgt aacgatgacg gcggcaaagg
    11521 tgatatgcaa caggccaact ggagtaatcc gggatggccg acgttgggct tttatcggac
    11581 caactctgac ggcgatgctt gtacaaccga cagcagagag agctataacg ccttaagctg
    11641 ttatgaaagt atttccgcga cggtttcaca gaattttgtc ggctggaata tgatgctggg
    11701 ttatacccgc acacaaaata acactgatga tagtttgcgt tgggataaac agcagagctt
    11761 tgaaaataac tatcttcgcc agacaacagc gcaaagtatc tctgaaactg tacaacttag
    11821 cgcttcccgc gcttttgtga tgcgtgactg gattttgagt acgtcagtcg gtgttttcca
    11881 tcgtaatgac aacggtggcg ataacgacga caacggcttg tacttatcgt tttcgttatc
    11941 tgacacgcca acgatggata gcaataacaa cagccattca acaaatgtgt ctacggatta
    12001 tcgttatagc gaacaggatg gcgatcaaac gtcatggcag ttatcgcata ccttttataa
    12061 cgattcattc agccataaag aacttggcgt aaccgtcgga ggcctgaaca ccgataccat
    12121 aaacagcgcg gttaacgggc gttgggatgg gcaatacgga aatgtctacg ctaccgtatc
    12181 tgatagttat gaccgtaaga atcatgatca tctttcggcc tttacgggca cttatagctc
    12241 tacgctggct gtcagtcgct atggcgttaa tttgggagcc agtggtacag acgatttgct
    12301 gggtgcggta ctggtggatg tgaaaggctt ctctgaacag gatgaagaga gtcaggatct
    12361 gcaactcgaa gcgcgggtgg caggcagccg aactttgcag cttggtcaaa gtgacagtgt
    12421 gttgttccct tatcctggat ttcagtctgg ttttgttgag gttaacgaca gtagccaggg
    12481 caatcagcag gggacaacaa acatcattaa cggtgcaggg aatcgtgaat taatgttgtt
    12541 gcctggcaag ctgcgctatc gcgaagtgtc tgccagcttt aactacaact atatcggtcg
    12601 tttattactg ccggcagcgg tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt
    12661 actggtagct gaagatggcg gatttacact tgaaattaac ggcagcgaaa aagagctcta
    12721 tctgctttcc gggcagcaat tccttaagtg tccactgagt gttgtaaaga aacgcgccag
    12781 cattcgttac agcggagatg ttacttgtag tgtggtgact tattcacaat taccggagtc
    12841 gattcaggtt caggcacagt tgaaacagcc taaattacgt ggaaacgttc agacggcgca
    12901 aagggaggtt gcaccatgag aaatcgattg attgcagcga tattgggctt gtttggcacg
    12961 ctcactggcg ttcaggcagc tcctgacgtg accagtgaaa ttacgtatga tttggcatct
    13021 ggtagagcgg attattactt ctggaaggat gaagcgtcgg caggaaataa tggatatatg
    13081 tggtatgaat gttcgtatcc tgacctccaa caaacctgta cagctaatgg aaatatatcg
    13141 acagtacaaa tctatttaac tgaacaacgc agtgggatgc gttggccggt aaaactcaaa
    13201 ggatttaaaa cagccattgt aagtagcgat gaagcgccgc caggatgcaa ggggggcaaa
    13261 gggcttcaga cgaatcttaa ggattctaat agatcttcat gtacagaaga tggtcaacat
    13321 tattatatat acgatacaaa gtttcttacg ctctaccttg agcagacaga gatgaagaat
    13381 ttgccgattg gtggcgtctg gaaggggaaa gttaaattac attcgaacag cccggcccag
    13441 gactatttcg caaatattac cctgaatacg ctcgacccca accatattga cgtgttcttc
    13501 ccggagttcg cccacgccac gccaagggtg cagttagact tgcatccaac aggaagcgtt
    13561 aacggcagca actacgcgca agatctgacc atgttggaca tgtgcctgta cgatggtttt
    13621 aacggtaatg ccatcagtta tgaaatcatg ctcaaagatg aagggcgacc agctgcaggg
    13681 cgcagagacg gttacttctc tatctatcgt cagggaggga ccaccaccga cgagggagaa
    13741 cgcattgatt accgggtcaa aatgtacaac ccggaaaccg gtgggcaaat tgatgtgcgc
    13801 aataatgaaa atatggtctg gaacagcatt aacctgaaac gtgtgcgtcc ggtggtactg
    13861 cccggtattc gctatgccgt gatgtgtgtg ccgacaccct tgactctggc agtcgataaa
    13921 ttcagcgtga tggataaaca ggccgggtac tacatgggca aattgtcagt aatctttacg
    13981 ccttccttgc caaccatcaa ttaaccctgc aaaccggaca aacgcttttt tctatataaa
    14041 cgacgaagcc gtctgataag gcggcttcgt cattacaact taagttaaac gttaatgttg
    14101 cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc gccggtttgt tctgcaacac
    14161 ggtccagata accagcgcac ccagcaggtc aaatactgcc agaactgcga acagcgggct
    14221 gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca aacagcgtac ttgccagcca
    14281 tgcggacatc ccggttaaac cgttcgccgt cgccacttcg ttacgaccga acacgtcaga
    14341 agagagcgta atcagcgcgc cagacagtgc ctggtgggca aaaccaccga tacacagcag
    14401 cataattgcg acatacgggt tggtgaacag accgatcata cccgggccaa tcatcagcac
    14461 agcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa accaacgctg
    14521 gaacagcggc ggcaggtaac caccgaggat acaaccgagg tcagcaaaca gcatcggcat
    14581 ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg
    14641 gatccacgcg ttaaaagtac cccaggccgg ttctgccagg aaacgcggca gcgcgatacc
    14701 ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgccg tgctcacctg
    14761 gtgctgggct tcctgaccat taataatata gtcgcgttct tcatcggtca gatgcttctg
    14821 gtcgcgtgga tgtttataga aaatcagcca tgccatcgcc cagataaagc tcaatgcacc
    14881 ggagatgata aatgccatct gccagctgtg cattacgatt gcccatacca ccagcggcgg
    14941 cgcaatcatc gcaccaatcg aagaacctac gttaaagtaa cctaccgcga tggaacgctc
    15001 tttcgccgga aaccattcgg agctggcttt cagacccgcc gggatcatcg ctgcttccgc
    15061 ggcaccgact gcaccacgag caatcgccag gccaccccag ctaccagcca gcgcggttgc
    15121 accacagaac acggcccaca gcacagcaaa cattgcataa ccgattttcg tacccagcac
    15181 atccagtaca taacctgcta ccggttgcat gaccgtataa gcagcagaat aggctgcgat
    15241 gatataggaa tactgttggg tggagatgtt taactcttcc atcagagttg gcgcagctgc
    15301 cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat
    15361 ataccaacgt aaccctttaa ttttacgcat gtaaaacctc atcgttttgt tatatccgca
    15421 ggaagcgcgg ccatcatccc gccagaggct gaagatgtta gttacgaccg ggtaaccgaa
    15481 aaaaaaccgg cacgccccga acgttgccat gaaagtttcg tgctaattcg gcttccgtac
    15541 cggttaaccc gattcatcta acaaatgaat gaatcggcaa tttgcattcc gtcgacttat
    15601 gatttgcgac ggcagaaaga taacttgtca tacaacttta aaaggtgaga gccatcacaa
    15661 atgtgcgaat atttgtaggg acattacctg acgacagcga ggccagtact ggcgcggcct
    15721 gcagagagat ttaccacttt gagagtaatt tttttaacta cgtttattga tctaactcac
    15781 gaaaatatct tcggactctg gaaattggtg tgataacttt gtcagcatcg caccataagc
    15841 aagctagctc actcgttgag aggaagacga aaatgactcc gtttatgact gaagatttcc
    15901 tgttagatac cgaatttgcc cgccgtctgt atcacgacta cgcaaaagac cagccgattt
    15961 tcgattacca ttgccatttg ccgccgcagc agattgcgga agactatcgt tttaaaaacc
    16021 tgtatgacat ctggctgaaa ggcgatcact acaaatggcg cgctatgcgt accaacggtg
    16081 tggccgagcg tctgtgtacc ggtgatgcgt ctgaccgtga aaaatttgac gcctgggcgg
    16141 cgactgttcc gcacactatc ggcaacccgt tataccactg gacgcacctc gaactgcgtc
    16201 gtccgtttgg tatcactggc aaattgcttt ctccgtcaac tgccgatgaa atctggaacg
    16261 aatgtaacga actgctggcg caggataact tctccgcgcg cggcatcatg cagcagatga
    16321 acgtgaaaat ggtcggcacc accgatgacc cgatcgattc tctggagcat cacgcagaga
    16381 tcgccaaaga cggctctttc accatcaaag tgctgccgag ctggcgtccg gacaaagcct
    16441 tcaacatcga acaggcgacc tttaacgact acatggcgaa gctgggcgaa gtttccgata
    16501 ccgacattcg ccgctttgct gacctgcaaa ctgccttgac caaacgtctg gatcacttcg
    16561 ccgctcacgg ctgtaaagtg tctgaccatg cgctggacgt ggtgatgttt gctgaagcga
    16621 acgaagcgga actggacagc attctggcgc gccgtctggc tggcgaaccc ctgagcgagc
    16681 acgaagtggc acagttcaaa actgcggtac tggtgttcct tggtgccgaa tatgcacgtc
    16741 gcggctgggt acagcagtac cacattggcg cgctgcgtaa taacaacctg cgtcagttca
    16801 aactgctggg gccggatgta ggctttgact ccatcaacga ccgtccgatg gcggaagagt
    16861 tgtctaagct gctgagcaag cagaacgaag aaaacctgct gccgaaaacc attctctact
    16921 gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat cggtaacttc cagggcgaag
    16981 gtatgccggg caaaatgcag ttcggttccg gctggtggtt taacgatcag aaagacggta
    17041 tggaacgtca gatgacccaa ctggcgcagc tcggtctgct gagccgcttt gtcggtatgc
    17101 tgactgacag ccgtagcttc ctgtcataca cccgtcacga atacttccgc cgcattctgt
    17161 gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc ggcggacatc aacctgctgg
    17221 gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga ctacttcgcc attgaactga
    17281 actaaggtct ggggttgata tgcaatacat caagatccat gcgctggata acgtcgcggt
    17341 cgctttagca gatttggctg aaggcacaga agtcagtgtc gataaccaga ctgttacgct
    17401 gcgccaggat gttgctcgtg gacataaatt tgcgttaacg gatatcgcaa aaggggccaa
    17461 cgtcatcaaa tatggcctgc cgattggtta tgcattggcg gatattgcgg cgggggaaca
    17521 cgtccacgcc cacaatacgc gcacgaatct gagcgatctg gatcagtatc gctatcaacc
    17581 tgattttcag gatctgcctg cgcaagcggc agatcgtgaa gtgcagatct atcgtcgcgc
    17641 taacggcgat gtcggggtgc gtaatgagct gtggatcctg ccaaccgtgg gctgtgtcaa
    17701 cggcatcgcg cggcaaatcc agaaccgttt cctgaaagag accaacaacg ccgaaggtac
    17761 cgacggcgtg ttcctcttca gccacaccta cggctgctca caactgggcg acgatcacat
    17821 caacacccgc accatgctgc aaaacatggt gcgccacccg aacgcgggcg cagtgctggt
    17881 gattggtctg ggctgtgaaa acaaccaggt tgccgcattc cgtgaaacgt tgggcgatat
    17941 cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag gatgatgaga tcgaagccgg
    18001 gatcgagcat ttgcatcagc tgtataacgt gatgcgcaac gataaacgcg agccaggcaa
    18061 actcagcgaa ctgaagtttg gtctggagtg cggtggttct gacggtcttt ctggtattac
    18121 tgctaacccg atgctggggc gtttctctga ctacgtgatt gctaacggcg gtactaccgt
    18181 actgaccgaa gtgccggaga tgtttggcgc agagcagttg ctgatggacc attgccgcga
    18241 cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac ttcaaacagt actttattgc
    18301 ccatgaccag ccgatctatg aaaacccatc gccggggaac aaagcgggcg gtatcaccac
    18361 gctggaagat aaatcccttg gctgtaccca gaaagcgggt tccagcgtcg tggttgacgt
    18421 gctgcgttac ggcgagcgtc tgaaaacgcc ggggctgaac ttgttaagtg cgccgggtaa
    18481 cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc catatggtgc tgttcagtac
    18541 tggtcgtggc acgccatacg gcggctttgt accgacggtg aaaatcgcta ccaacagtga
    18601 actggcggcg aagaaaaaac actggatcga ctttgacgcg ggtcagctga tccacggtaa
    18661 agcgatgccg cagttgctgg aagaatttat cgacaccatc gttgagtttg ccaacggtaa
    18721 acaaacctgc aacgagcgta acgacttccg cgaactggcg atcttcaaaa gcggcgtaac
    18781 gctataaagt cgaaatgagt tgttaataaa aaacggcgtt tcataatgtg aagcgccgtt
    18841 tgcttttcca tacctgataa ggatttatca tgaccgcgta ttggctggcc cagggcgtgg
    18901 gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca
    18961 aaaagcagct ttcggtctat agcgccgtta ttggcgtaca tttttttctt ctgggcacct
    19021 atcccgctgg tgccagtgcc atccttaatg ccattcgtac attgattacc ttacgcacgc
    19081 gcagcttatg ggtaatggcg atttttattg tgctgactgg cggaattggc ctcgcgaagt
    19141 tccatcatcc tgtcgaacta ttgccggtta tcggtacgat tgtcagtacc tgggcgctgt
    19201 tccgctgtaa agggctcacc atgcgctgcg tgatgtggtt ttcaacgtgt tgctgggtga
    19261 ttcacaactt ctgggcgggg tcgataggcg gcacaatgat tgagggtagt tttctgctta
    19321 tgaatggctt gaatatcatt cgtttctggc ggatgcagaa aaggggaatt gatccgttta
    19381 aagtagagaa aacccctccc gccgtagacg aaaggggtta aacaattaat tacgcagggc
    19441 gctatttgcc agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt
    19501 tgaagagttc agcgcggttt cgcaagagtc ctgtaatacg ccgatgataa agccgacggc
    19561 aaccacctgc atggcgatat cgttcgaaat accgaacata ttacaggcca gcgggatcag
    19621 cagcagagaa ccccccgcca cgccggatgc gccacaggca cacagagaag ccaccacgct
    19681 caacagcagc gccgtgggca gatcgaccgg gatacccagc gtattaaccg cagccagcgt
    19741 caacacggta atggtgattg ctgcgcccgc catattgatg gtggcaccca gcggaataga
    19801 aacggaatag gtatcgcgat ccagattcag cttttcacac agtgccatat tcaccggaat
    19861 gttcgccgca gagctgcggg tgaagaaggc atacacgccg ctttcgcgca ggcacagcag
    19921 caccagcggg aacgggttac gacgaatttt ccaccacacc agcaatgggt taaccaccag
    19981 cgccaccagt aacatacagc caaccagcac gaccagcagt tgcgcgtagc cccacagtgt
    20041 ggagaaaccg gtggttgcca gggtagaaga aaccagacca aaaataccaa tcggtgcgaa
    20101 gcgaatgacc agtttcacca taaaggtgac ggcattcgac atatcgttga ccaggttttt
    20161 ggtggtctcg ttaccgtgac gcagtgcgaa accgaggcca attgcccaca ccaggatccc
    20221 gatgtagtta cctttcagca gcgcgtcgat ggggttggaa accatgctca ttaccagccc
    20281 gcgcatcact tcgacaatgc ctgacggcgg cgaaatatca cccgcgctac tggataaatg
    20341 cagggtagaa gggaaggcaa agctgaagac tactgcggcc agagcagcag agaaggtacc
    20401 cagcagatag aggaacaaaa tagggcggat attggttttc tgcccgtgct ggtggttagc
    20461 aatagatgcc atcaccagca tcaacaccag gatgggggca acggctttca gtgcgccgac
    20521 gaacaaagta cctaacagac caacagcttc cgccgcgggt tttgagatcc atgccagaag
    20581 aatccccaga acaaggccga ccaggatttg ttttaccagg ctgccatgag ccagacgccg
    20641 gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca
    20701 tccctggtgc gttctgtcga cgcatctttc aggatgtaac aaagtgtttg caaggtcgag
    20761 tataaggaaa gttacagctt cgggaagcga aaaatgctgg attttaaaga gaggtcatat
    20821 tttgcaactt aattgttaca tttgtttgac ataaacagag ggaaggactg gctactaagg
    20881 ctgattagat aacatatggg caaaactatt tggaatatga gaacggttgc gctcctttaa
    20941 tgtgttatgc catataaagt cactggcgca gggagcgcgc agggggcggc caatcgccgc
    21001 cgccccctgc acccccaggc tctggcgaac aaaatcgccg ctgcgcggtt ccctcagctt
    21061 atcccttgcg gctaccgggt cgggcgcaag gtaacgtccc tgtaagatgc gccctcgccg
    21121 cgcatccatg cgcgtcgccc cggcctccag ggaacgcttc ggcgattttg acgccaccaa
    21181 cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat agctcacctt
    21241 tgtctggaat gagttatctc tagtaatggc ttaatataca gcggatcatt ctccccgctg
    21301 cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca gaatgccaaa
    21361 caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca gcatcttgat
    21421 accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac gctctgctac
    21481 gcccgccagc aggaaataca tcgcacgcag gccgaggatt gcaaacaggt ttgaggtcag
    21541 cacaataaac ggatcagtgg tcacggcgaa gatagccgga atgctatcca cggcaaaaat
    21601 cacgtcgctc aattccacca gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc
    21661 attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg
    21721 tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg cgagcgccat
    21781 cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt cgaactgtga
    21841 aatcagccag ctgcctgtga agatcatgat ggtacgcaga acaatcgccc cgagcacgcc
    21901 atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga acaacatcag
    21961 ccagacaaag acgttatcga ccgccagcga tttctcaatc agatagcccg tgagaaaggc
    22021 cagtgcctgt ggatcggcaa cggcgcgacc ttcagtttgc accagatacc accagaaagc
    22081 ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag cctgtttcat
    22141 ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca gcataatggc
    22201 gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca tagaagttcc
    22261 ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt atgcatagac
    22321 ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg tattcctttc
    22381 gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg accggcaatg
    22441 aagttactcc cctttgcagg taacaaagta tgacagacca ttcagccagt caatggtctg
    22501 ttacatacct tttacactgt tttactttgg caattatagt tttacgctat ctgccggaaa
    22561 aatcacccca gtctggcggc ggatctcggt cagcagtttg gcggtgatgc gactgaccgc
    22621 cagccccgga tgatccacca gatgctcatc caccagggta gcgaacagct ctgcttcgta
    22681 gagcatggta ttaatatgct gcggctgggt gagatcctgc atttggctgc cacgcggcac
    22741 gaagcacact ttctggcatt cagacagttt ttcaatcacc agcgatcctg cttcgccctg
    22801 aatctcgctc gccaggacag aatcactgac ttttgagtgc tgcaaggtga cgctgaaatc
    22861 accgtaatcc atcaccacca cgccgtgggc atctacaccg cttgccagca aactggcggt
    22921 tgcctgcacg cttttcggct caccaaataa cgccaccgcc gacgccaggc agtaaaagcc
    22981 gatatccata attgaaccgt tagagaatga cggattaaag gtgttgggat tttcaccgtc
    23041 caggtaacgt tgatagcgcg aggaatactg gcaatagttg aaaaagactt tacgcaattt
    23101 gccgactttc ggcagcgcct ggcgcagcaa atgaaagttc ggcaggcagg cggttttaaa
    23161 tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat ccacttccgc
    23221 cagattcgac gccagtggtt tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg
    23281 tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa tggcatcgct
    23341 ttccgccatc gcttccagcg aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg
    23401 ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac cgctctcatg
    23461 ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga aacgtatcat
    23521 aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt tttcatcttc
    23581 gacgtgcgca gatacgcatt attcttttgc cggacagcca tgctcactaa acggcccaat
    23641 catgaccgat ggcccgagat atttcggctg gcgatggagg attttggcgt caatcacggc
    23701 gctgacacga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca tattgaggtc
    23761 aggcgcgtta aaaccgatag cgtcgatgcc gtactgtttt gccagccaga tggcgcgttg
    23821 attatgaaat tcttgcgaga taatggtgat atggttttca ccaaaaacct ttttggcacg
    23881 cactaccgaa tccagcgttg agaatccggc atagtcgcag aagatcactt tcgccggtac
    23941 gcctttagcg atcaaagcct gctgcatacc tgaggcttca tcgtaatttt tacgtccgtt
    24001 atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg ctgcggtatc
    24061 aatacgtcgg gtaaagtagc gattacccgg cctggccccc agcaataagc ccacgttacg
    24121 cgccggaact gcgttgacat cgctccaggt cagctgcttg ctggcgttga ccataacgcg
    24181 atcggcaata aaaatggtcg ctcctgctat aagcaacaac aaacagccaa tcttaagagt
    24241 ccggcgagaa aaacaaatac ggagaagaag acggggctgc atggtcgtta tccttaacga
    24301 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    24361 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    24421 ggtggtactg cggctccata tggcagcaca actggtaaaa cgctttgtta tgctctttct
    24481 ccttcaggtg cgccagttcg tgcaccacga tcatacgtaa aaacggttcc ggcgcgttgc
    24541 gaaacacagt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcacacgag
    24601 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    24661 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    24721 agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    24781 ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg ggataaccct
    24841 gaagataagt aagattgttc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    24901 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    24961 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    25021 acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca cagaaatccg
    25081 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    25141 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    25201 cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    25261 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    25321 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    25381 tgacattcac acttccacgc tggagctgtt cgagaaagtg ctcggcccga ccaccactac
    25441 gctggcatgg aaaaaagcgc gcctgattaa ttgcactttc aatgaaccgc cgctggccga
    25501 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc acaaccatgc
    25561 gaatgtcttc tcccgcaccg ggctggatat tggcgcacgc ttctttatgc aacatctgcc
    25621 agagaatctc gaaggtgaga ttgtcgatct cggctgtggt aacggcgtta ttggcctgac
    25681 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    25741 cgcttccagc cgaatgaatg ttgaaaccaa catgccagag gcgttggatc gctgcgagtt
    25801 tatgatcaac aacgcgctct ccggagtgga gcctttccgc tttaatgctg tgctctgcaa
    25861 cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga tgttccacca
    25921 cgcccgccgc tgcctgaaaa tcaacggcga actgtatatc gttgccaacc gtcacctgga
    25981 ttacttccat aaactgaaga agattttcgg caactgcacc accatcgcca cgaataataa
    26041 atttgtggtg ctgaaaacag tgaagctggg gcgtcgtcgg taagattggg agttacctgc
    26101 cggatgcggc gttaacgcct tatccggcct acgttcagca cgttactccc aggccggata
    26161 agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa
    26221 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttgtt caacgtttat
    26281 gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta
    26341 ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc
    26401 tgtggcacct tttctggcgg gtattcagaa cgaggaacaa tacacccagg cgctggaact
    26461 ggtagatcat ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgcaaa
    26521 aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc
    26581 catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt taaccctttc
    26641 cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa
    26701 attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt
    26761 tattgattaa cgcgacgtgc cggatgcgtt gcatccggcg cttcaaataa cgatttaaat
    26821 ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac
    26881 atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc
    26941 gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc
    27001 gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat
    27061 cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa
    27121 cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg
    27181 gatctgcatt ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca
    27241 ctcattacag aacctggcga tattctggct ggtggattcg cccggctgac ttaaatacat
    27301 cgccgtatca aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc
    27361 tttgtcgcgc agtacatcga gataactcaa taccttcgga tgatcgatcc cgtcgatagg
    27421 cggaacgcgc ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc
    27481 cgccgtcacg gtgtgattga gtttcagcgt cacgcccgtc acttcgatca tccggtggta
    27541 atagcgcagc gtttcgtaaa actcctcttt gccggggatc tgtttggcga tattaaactg
    27601 cccgccgatc tcgctgtggg catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt
    27661 aatggcaaac gccagcccag caggtcccgc accgaccacc gccagatttt ttttctgcac
    27721 ggcaggaagg attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt
    27781 gactttgcca acgaagatct gatcgaggca ggcctgattg cagccaatgc aggtgttgat
    27841 ctcatcggct cgccccgatt gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg
    27901 tgccatcgat accatatcgg catcgccgcg cgagagaatg tcgtcggcaa cctgcggatc
    27961 gttaatccgg ttggtggtca ccagcggcag cgagacgtgg cctttcagtt tgcgcgtgac
    28021 ccaagtaaat gcgccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca
    28081 gccaatgccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg ccagttctac
    28141 cgtttcggca aaagtgccgc cgccttcgac caggtcgagc atcgacaacc ggtagataat
    28201 aatgaagtcg ttgccgacgc gttcgcgcac cgtacgcact acttctacgg caaatcgcat
    28261 ccggttgcgg taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa
    28321 ttcgttgatc aaataccctt cggaccccat tacctctaca ccgtcgtatc ctgcctcccg
    28381 tgccagttgc gcgcagtggg cgaaatcgtc gatcagttgc aggatctctt catgcgtgag
    28441 ttcgtgagga acgaaacggt tgatgggggc ctgcaatgcg gagggagcga ccagatgcgg
    28501 ttggtagctg tagcgcccgg tatgcaaaat ttgcagggct attttgccgc cttcctgatg
    28561 taccgcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc
    28621 accttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac
    28681 gccgtgacgg gcgcgttcgg cataaaacgc tgccagccgc tcggcaccgt ccgggtattc
    28741 ctccagcccg gtgtgcattg agcccatcaa cacgcggttt tttaacgtgg taaaacctaa
    28801 atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt aaaataattg
    28861 ttatgtggtc ggatgagttc taatttagcc gtcgccggag aaaagggaaa aggggagtgc
    28921 ggggtttgtg atgggattca aaaagaatac gcccggtggt aacgccggat gcgacgcttg
    28981 ccgcgtttta tccggcctgg gggattgcac atgtagggcg gataaggcgt tcacgccgca
    29041 tccgccagtg gcgcggtgca gttgccggat gcaacgcttg ccgcgtttta tccggccagg
    29101 aggattgcac atgtagggcg gataaggcgt ttacgccgca tccgccagca gttgcaaatt
    29161 attgcttacg gaaaaaatcg ttatacaaca tatacaaatg cgcggcactc caggagaaat
    29221 ttggtgcgcc ttgctgtgcg cctgtcagcg ggttgtaatt ttcctgaatt ggtccatcgg
    29281 cggttaaccc tttggcgtgc cggaagaacg tatccgccag cttcagggca tcatcacgat
    29341 aaccgtaacg ctccatccct ttcagaccaa accagaactg atccacccat acgcgcccac
    29401 gccagtagat atcagcgcca aaagccggat tggttaacgc cgccgttccc agcgggacaa
    29461 aggtgttgaa ctctttagga tcgagcatca ccttcaccac cgcgtcggca ttggcctgcg
    29521 ttgccgcacc gttaaacagc ggcgaccagc cttccggccc tttaccgcgc tcaacaatcg
    29581 gtttgcccgc gcagccgttc gccagtggtt tatcttcaat acgtacgtca tagtagaact
    29641 gggtagtcgg gtcgaacata caggtgttga tgtagtccgc gagctgctgt gccaactggc
    29701 gatagcgttt ggcctcttct ggcttaccga ggatggttgc catctccgcc agataatggt
    29761 tatcgctgta catatagctg gcctgatcca ccgactcctg caatagcgag tagcccagca
    29821 acgttccgtc ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta cgtttgccgc
    29881 cgcttgcaac atatttatcc agctgttctt tgtcgataaa accaaagacg gcggcatcat
    29941 cacgccccga ttcccacgac gcagcgacct gtgccggaat ttccagactg tcgtactggc
    30001 ctttctccac cacgcgggcg tagttgttca gcccagactg cgtctcttct ttgttgcctt
    30061 tcttcaccgt aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg cgggtcgcgc
    30121 catattccgg cacgccgttg ccgttgtgat cgcggttacg taaccaccag tcgtgatagg
    30181 ccaccagttt cgggtacatc tctgccaacc aggctttatc ctgggtgacg ttgtacactt
    30241 ccatcaccga ccaggcggca aggctgggtt tggtattacg ttcgttccag ttgccgccat
    30301 cgccaccacg ctcggggcta aggttccacg ctatcaggtc ggggacaaag cccacatcct
    30361 gcggacgcac gctatcgcca ggctggatct gccaggagaa gaccgcgcgg atattctctt
    30421 tggcgatgtc cggattgaaa tgcgccatcg caaacgcctg cttccaggta tcccacggcc
    30481 aggtctgatt gccggagaac cagcgcccgg tcaccgacgg tgtaacggtg ttaaatttca
    30541 ccgcaccgcc cggtgagcgc cagttaccgt tgagcgtttc gatcgctttc accgcgacgc
    30601 gcgtctgttc cggcgtcgca tccggattgg ttaacccttt cttcaggtat tcttcccagc
    30661 gttgctgcga ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg atctgcattt
    30721 gctctttgct aacttcctga gcggtcagca gatgggaata ggtggtgtag agcgtggtcg
    30781 aaccgttgat atgcgcctta ctggtaaagc gattaccgtt gatttcagtc tgcaccggca
    30841 gggatttatg cacctgatat tccgattcgc cggaggtcag cagatcccag gtggcgcgca
    30901 ctttgccaaa ggtgactttc aggccatcac gggtggcgct gattttgcgc tgatagtcag
    30961 ggtattcgcc agcaatggtt ttatcggaaa gcggtttccc ttctttcgct tccagttttt
    31021 ccagcagttc gccgtcccac accagatcca gcggtttatt gctggtgatt ttggtttcca
    31081 gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc gacttgcaca tcttttgctg
    31141 tcagtttttg caccagcgca ccgggaatac tgtatgcctc cagcgtaaag tcgactttct
    31201 tgccgtcctg ccagacggtc aggcggtcga aattgctggc cataaagttg atgtactctt
    31261 ccgtcagcag cgcaacgccc ggaaagccgc ccatggtgtt agggccgtct ggcaacagat
    31321 gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg ctggtgatcg tcgtaatcgt
    31381 aatccttcat gtactgcggc gcgccagtac ggttaatcac gtttttataa ttgtcggcgt
    31441 tagcggcatg ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt aaaatagttt
    31501 ttattttcat gaaattacag tccttattac cagtagaaat attgcatcag gaagatttgg
    31561 tcgctgggat cgtgagaggc atttttcatg tagaagcggc tatcaaaagc cgtggcgaaa
    31621 cgtccgccgc cgtattcgta ccagatacca aactgcatga aagaggtggt ttcatcgtca
    31681 gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg agagataaac gccctttaac
    31741 gcttcataca tggcgctcac catcatccca ctttcacttc ccggtacgtc cagcccgtcg
    31801 gcgcgtccgg tatggtgcca ggcgcgagcg gcaaagttag gagttagcag gcccgtgaga
    31861 tagagctgtc ctgtgccgtc ggtgatttcc agaccgttca tccaggtgat gccttcggca
    31921 aggtcatact ggatgtaacc gttcatcatt gccggatagg tgtatttgtc gtcatagcga
    31981 tcgtacttgc caaaatgtag ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg
    32041 gtgacgctat aacgaaagtt gccgcttaag ttctgcacct tcagcgcggt cataatgtcg
    32101 cgggtattgg gaatgacgta gcccagatcg ggggtgaagt cgccccacca ttgcaggtca
    32161 tccagcgagg aatcctgacg cagactgagc atgatttcgg tgccatcatt ggtacgataa
    32221 ccgccataga aacggttaat tccgccctcg aaaccacccc agctatggtc tggcacccag
    32281 gcattgccgt cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag
    32341 gagtcgtact tcatctccag gccaccttca atataggtac cgtcgctgta tttatgttta
    32401 ttctcaccct ctaaatccat atatccacca acccctaatt cgccgtaaaa acgaaatgaa
    32461 ggagaagatg tcgaggtaat tgcaggtgtt tttgtgtcgt catgttctgc cggacgtgct
    32521 tcttctcctg ccgcagaaaa ggcgtagcaa aacggcagag caagtaatgc tctgcatggt
    32581 gcagtgatta atttcattgt gttaatccat gagctaaaga aaaagtacag ccttttatcg
    32641 ttgtaattat aaaaatataa aggcagaaac tattttccct ctcctcatcc aggaagaggg
    32701 aaaatgcctg ctcatttttt attattctgc ttaatcaaca ttgctggctg gcgtcgcttc
    32761 aatatggcgc tcttcagccg ttaatccctt tatatattta ctgtatttcc accacgcaaa
    32821 gccaaggaag ataacaatac cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc
    32881 agtcgggaat gtcgaagcga caaaccctac ggcaaatatc gcaatcagca ttgaaacaac
    32941 gataataccg gtgcggcggg agcccatacg gaaatcgcgt ggcaggtgat ctaatttggc
    33001 gcgtaaattc aggtaagcca gcatgatgaa taacggcgga agcatggacg ctgcggcggt
    33061 catattaata atagtattca tcagatcctg cacggtattg gaaccgagca tcgggataat
    33121 catcagcggg atgacgatca ggaactggat ccacgctgcg cgcgccggaa cgccgttttc
    33181 gttcagttcg acggttttct taccaaagat gccttccggg atttcggaga agaaaatttt
    33241 caccggcgtt gcggtccaca tcagcaggga accgaacatc gcggtaaagg acaccagacc
    33301 gacaaagcga ttcatcaacg cttccggtag accaaaatac gccgccatgc cgtggaatac
    33361 ctgcaccgat ccgccggtaa atttcaactc tttgctgctg acgaagacgt taatcagcac
    33421 cgaggagacg gaatacagta cgccgataaa aatcccggcg aggatgatca ctttaacgaa
    33481 cgatttcgaa ccgcctttga cgtcgttaac gtacacagcg acggactccg cgccacctgc
    33541 ggccataaag atccaggtgg taacgccgag gaacgcccag ttgaagttcg ggatcatcgc
    33601 gtcaacggtg atggggtcag caggctgtac gccgccaacc agcgccgtac ctgccagtaa
    33661 aatgtaggag agcgttaaca gcagcatcag cgttgaagtg acggaggtaa ttggccccag
    33721 cattttcgcc ccgttggtgg aaacccaggt ggagaaggcg aacagcacca tactgataat
    33781 ggtggtggca accggcgtca taatatattc gtagccgagg aaggcatacg aagcataagc
    33841 aataacgcgc ggcaacagtg aggtgaaaaa gaacaggttt acgaaccagt aggtataggc
    33901 agtaataaat gcccaacgtc cgcccagcga actttttacc cacgcgtaga caccggcttc
    33961 tgagttttta tttaacgaaa caaattctgc gatgatcaga cagaagggaa taaaataaaa
    34021 aatcgtcgcg aggaaaaaca tcggtgccga ggccagtcca agctcaatat tattattgat
    34081 aacgttgtta aagctgtaaa cggcggcaaa agtcagcgag agcaagccga atttgccgat
    34141 tgtattacgt ttggtatcag acataacaca tctccgaaaa tgtgtagggt agcggtggcg
    34201 gcaaactcct tttgccgccg tagttgttat ttgttatgga aataaccatc ttcgatggtg
    34261 actttgagaa ccactttttt gaccgcgtta ttgcagataa accgatacgc ttcatggata
    34321 tcgcaaataa cgatttgccc ttcgtggacc tcaacggttt ctccgcagcc ttttaaatat
    34381 tcacggtcag tttcatcacg ataatattcc actacctgta atgtctcttt cggcgcatat
    34441 tcaatttttt gctgcccttg caggtagtaa tgcacttcaa aatagcgtcg atgcccggta
    34501 aatagcgcat cggtcgcgga gtctgtctcc acgcggtaag tcaatgagtc accgatggag
    34561 tgggcgacgc caggctgaat gttgtcgata ttttcaatcg cttcaacgca gcgttgccac
    34621 ttcttgccag aggcgtaaat ctggcggaac tgttctaagt tatcgatgat cctcattgct
    34681 tattctcgct gtgcaaattc gtggaaaaga accctgcgcc gaactcatac gacgccaggc
    34741 tttgcgcggt agcttctccg ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac
    34801 ggaaccagac gcgccaggag tccagcacct cgctgcccca ggagttggag ccgaggccaa
    34861 gcagctggtg gtcgaggttc agggtgatgt catcactgcg ctgcagctcg ttacagtgct
    34921 gggcagcgtg gatgttttcc tgggtatagc gccaggcgct gaagttaatt gggcgctgcg
    34981 gaaccaccag cagaccgttg ccgtggcggt tagtcagtgc cgtccagcgg acatgctgac
    35041 ggttgccgtt gttctgcggg aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc
    35101 agatatcgat gatgttagcc tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt
    35161 aatacgccac ctgatcgtat tcgccgttaa tccccatggt gaaaccgatg cacggaatga
    35221 tgtgcggata gtcgccgtaa cgctcgccgg aaagcgccac gttaacctgg ccatcggcag
    35281 cgatgcgcca gatgtaggtg cagcgcatcc cgaagtcaaa caccggcggg gcaataactg
    35341 tgcggctgat gatcaacact tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt
    35401 cctgcatgat ctgcaaatga ttcggttgcc acagcccttc gtactcctgc ttgtggttgt
    35461 cgatcattgg cttgaagaag ttgatctttg gctcgcgggt cagcagcgac tcgccattca
    35521 cctgccagga tgtcggtttg ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc
    35581 gaacggtgca gctcaaacga tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga
    35641 aaggcactgg ttgcgcggtg ttttccttca gcgggaactg ataagtggcg atagaatgcc
    35701 cggcttcgct gtagcgggtg cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc
    35761 gggcgtccag ctgcggcagc gtgatctgca agggggcttc gctgttcggc gcaacgtcgc
    35821 gcagtttaat ctgctgcgtc gcgagcgttt caccttcgac gcgcacctct gcgtgcaggg
    35881 tgtagtcatc aagcgtggta aaccacagtt tattttcgac tttcagctcg ccgcgagtca
    35941 gatccagcgc gtggattttt accggcgcga taacctgttt gtactctttc aggcccggtc
    36001 ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt
    36061 cgccgtagtc gccgccgaat ttataccaga cgttgccgtt gtcatcctgc gcctggatcc
    36121 cgtggtcgca ccactcccaa acataatgtc cctgaataca atcgtgctta tagaagacgt
    36181 tctggtactc cgtcagcccg cctggtccgt tccccatcgc atgagcatat tcacagatga
    36241 tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca
    36301 tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatgc accagtcgcg
    36361 tgtcatccag cgccttcgct gcgtggtaca tcgcgcggat gttacagcca tagccggatt
    36421 cattgcccag cgaccagatg atgatcgacg gatggttttt ctgcgcgtga atatggcgaa
    36481 caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc
    36541 cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac aagccgtaga
    36601 tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga
    36661 tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc
    36721 ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga
    36781 tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggcaccactt
    36841 ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt
    36901 ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt
    36961 caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacagggtat
    37021 attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact tcgcaggaaa
    37081 gtgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg
    37141 ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgctgaccac cacatatcct
    37201 ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac aggttgtcgc
    37261 cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac
    37321 ccacatactg accgttaaca tagacttcaa aataggtttc gacgccgtca aatttaatca
    37381 gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg
    37441 ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt
    37501 gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag
    37561 ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa aagtggaaat
    37621 tccactgacc gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt
    37681 gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga
    37741 gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca
    37801 tggtgatggt agtttgtgta attgctgggg agtttagtaa aatttttact aaaaagtagc
    37861 aggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg tccggcaaaa
    37921 acgattgccg gacagtgtgt aaggggattt aacgggtcgt gccgcgcagt tttaatttgc
    37981 tgggaacgaa gactaacagc ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt
    38041 taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc ggcggaaagg
    38101 taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcctgt gggatgttca
    38161 ggcctcgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca
    38221 gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa cccgacgaac
    38281 tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt cggccatatt
    38341 ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg caaataaaac
    38401 caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg atgcgtgcca
    38461 gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata aaacagatat
    38521 tgtcggtcaa cgcacaggcg gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa
    38581 taccggtgac gtttttaatg tctggtaatc cgctgtgttc ataacagttg gtgagctcaa
    38641 tgcccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag taaggatcgt
    38701 tgatctccag ctcctgttgg tagctgtaga tagccagaat atggtgttgg ttgactgcac
    38761 ctgtctggag tttacgggca ctactggtct tgtactccag cttttcggcg atctcgagaa
    38821 tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag accctggata
    38881 ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt tagtgttgcc atgtttacct
    38941 tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt
    39001 ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc tcacgaatgt
    39061 gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt tgctatagtt
    39121 tagtaaaatt ttacccgaag ggaggaaaca atggaaaccg tggcttacgc tgattttgca
    39181 cgtctggaaa tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag
    39241 ctgtacatcg tacaggttga tgtgggggaa aaaacactgc aaaccgtgac cagcctggtg
    39301 ccgtactaca gcgaagaaga gctgatgggg aaaacggtgg tggtcttatg caatctgcaa
    39361 aaagcgaaaa tgcgtggtga aacgtcggaa tgcatgctgt tgtgtgcgga aaccgatgac
    39421 ggcagtgaaa gcgtgttgtt aacgccggag cggatgatgc ctgcgggcgt acgcgtcgta
    39481 taaaaagatc ggatggcgac gtcgtatcgc catccgattt gatattacgc ttcttcgaca
    39541 cttactcgca tggccgccag cgccttacgc gccgctttga tcaccagttc acactgttca
    39601 atggtcagtg tcagtggcgg ttcaatgcgg atcgttttgg cgttattgag cgttccggcc
    39661 accagtacgc gctggcggaa catctcgctg gcaaagttat agccgatttc gttatcaaca
    39721 aactcaattg ccatcaacat ccctttacca cgcgcttcct gtaccagatc gggatattcc
    39781 cgcgccagtt gacggaaacc gtccagcaac atatcgcctt tttgctcagc ctgagccggt
    39841 aagttctgct ccagcaacac attgatggtc gccagcgccg ccgcacaggc cagcgggttg
    39901 ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca gaactgaaaa cacctcttca
    39961 gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga gcgctttggc aaggcagagg
    40021 atatccggct gtacgttctc atgctcgcag gcgaacatct tgcccgtgcg ccccatgccc
    40081 gtttgtactt catcgaggat catcagtgcg ccgaactcat cgcatagctt acgtacggcg
    40141 gtgagatagc ccggcggcgg cagaattacg ccaccttcac cctgaatcgg ttcgaggatc
    40201 accgcagcca catcatcacc ggtttttttg cactcgttaa gagccgtgcg catggcttcg
    40261 atattgccaa acggcacatg acggaagccc ggcagcagtg gcataaacgg tttgcggaag
    40321 gtcgatttcg ccgtggccga cagcgcgcca agtgatttac cgtggaacgc gccgctggtg
    40381 gcaataaaag taaacttgcc gcgcggtgac tggtaagctt tcgccagctt cagcgctgct
    40441 tcaacggact cggtgccgct attacagaag aagctgtatt tcagtttacc gggcgttagc
    40501 gcagcaaggg ttttcgccaa catcgcccgt aacggatcga gcagctcctg gctgtgcagc
    40561 ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa ctggattacg gtgccccacg
    40621 ttgaaaattc caaaacctcc caggcagtcg ataaactcct gtccctgggt gtcgacaagc
    40681 gtatttaaac ctcccgcttg ccactctacg gctccgtaat ccccgccggc ggtaacagat
    40741 ttgcgatact ctaaaaaccc cggattgaca tgctctttga agtattcaat cacctctcgg
    40801 ttaagtgctt tcatctcctc atgatccagc gttcgcttct caatgagatt cagggcgtgg
    40861 gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt tcaaaatatg ctccggaggc
    40921 tcgcgtatca catgataccg atttaagtat tgcagggatt gcgccactcc ggctgcgatc
    40981 gcgaaaatcg ggataaacac aaattcggtt atcatttgtg caatatttaa tgctatctgt
    41041 taacatttgt tgttattttg cacggttttt gtcgggtatg gatttgatga ttgctgcgca
    41101 aatgcacgtt ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt
    41161 atagtaacag agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat
    41221 tgcgatctaa atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg
    41281 acaagaagtt aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg
    41341 tcacccagca aaataccccg ctggcggacg ataccactct gatgtccact accgatctgc
    41401 aaagctatat cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag
    41461 agttacaagg gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg
    41521 cggatatgtg gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc
    41581 gcaaaaatgg cgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa
    41641 aaatcagtgg ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg
    41701 agccgctgta caaagcgctg aacgccggac gtaccagtaa gcgtattcat aaaggcctgg
    41761 tggtgcgtaa aggctggctg ggtaaactgc cttcattacc gcttcgctgg cgggcgcgtg
    41821 gagtgatgac cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg
    41881 tggtgacgta tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc
    41941 agattgtgcg cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc
    42001 gtaatagtgt tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag
    42061 ggcagcttgg cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg
    42121 tcagaaatgg cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc
    42181 agacagttga taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga
    42241 aacagaactc tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg
    42301 cagtgcaggg tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca
    42361 gtacccagcg cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata
    42421 ttctggccct gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg
    42481 cagtggtggc aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata
    42541 ttcgcaagct gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg
    42601 ccgccggacg gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg
    42661 cccagattag ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag
    42721 tggatgagct taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg
    42781 tgtcggcgat ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt
    42841 aatcggtatg ccggatccgg cggttggagc acaatgcctg atgcgatgct ggcgcatctt
    42901 atcaggcctg cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc
    42961 cggcaattgc accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa
    43021 gcggcgcggt cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg
    43081 ttgacgcgta aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc
    43141 tggcgcagcg ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt
    43201 tcgcggtttt cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca
    43261 ccttcctctt cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgttgggtag
    43321 atgacgcccg ggcttggggt gtaattcccc tgggttaggt tctcaatcgc tttaatcaat
    43381 tcgtaaccgt ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg
    43441 tgaccaaaga aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcagcattga
    43501 ccgtgttcat gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag
    43561 cagccctcat ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata
    43621 tcagcctcct tttgcttgat tagatatatc taatttatat ctgatgtgag cactcttgca
    43681 agaataaaaa tgatttttaa atgtggactc atctcacacg taggccggat aaggcgttca
    43741 cgccgcatcc ggcgttgagt aatgatgcct gagaagaggc ggttcgtctt gtcagactat
    43801 agacatatca gttctacaaa tcgcttgcat ttatcatgat taacaatcat tatcatttgc
    43861 gaaaattatt tagatatatc tgattaccat cacgaaggcg ataacaatga ataactcccc
    43921 ccgctacccg cagcgcgttc gcaatgatct gcgcttccgt gaattgactg tgctacgcgt
    43981 tgagcgtatt agcgccggtt ttcagcgcat tgtccttggc ggcgaggcgc tggacggttt
    44041 tacatcgcgc ggctttgacg atcacagcaa actcttcttt cctcaatctg atgctcactt
    44101 tgtgccgcca acggtaacgg aagagggcat cgtctggccg gaaggaccac gcccaccgtc
    44161 gcgtgactat acgccgctgt atgacgaact acgccatgaa ctggcgattg atttctttat
    44221 tcacgacggt ggggtcgcca gcggctgggc gatgcaggcg caaccgggcg ataaacttac
    44281 ggtggcaggt ccgcgcggtt cgctggtggt gccggaagat tacgcgtatc agctgtatgt
    44341 ctgcgatgaa tccggaatgc ccgcactgcg ccgccgcctg gaaacgttga gcaaacttgc
    44401 cgttaaaccg caagttagtg cgctggttag cgtgcgggat aacgcctgtc aggattatct
    44461 cgcgcacctt gatggtttta atatcgaatg gctggcacat gatgagcagg cggtagatgc
    44521 gcgtctggcg cagatgcaaa tccctgccga tgattacttc atctggataa ccggcgaagg
    44581 taaagtcgtt aagaatttaa gccgccgctt tgaagcggaa cagtatgacc cacagcgggt
    44641 acgtgcagcg gcttactggc acgcaaagta actgatatat aaaaagaaaa aggctgacga
    44701 tttctcgtca gcctttgcta tatctggtgg cccctgctgg acttgaacca gcgaccaagc
    44761 gattatgagt cgcctgctct aaccactgag ctaaggggcc atggtagcgg attataaagt
    44821 aactccgtgt cgcaatccag ccattaccgt gcgcctgctg tttttataaa caatgtattt
    44881 tcaatccgtt atacttttcc tggtgatgtt agaaaggagt aaatatggtt gaggatattt
    44941 tggctccagg gttacgggtc gtgttttgcg gtatcaaccc tgggctttca tccgccggga
    45001 ctggttttcc ctttgctcat ccagcaaatc gcttctggaa ggtgatatat caggccgggt
    45061 ttaccgaccg tcagttgaag ccgcaggagg cacagcatct gctggattat cgttgtggcg
    45121 tcaccaaact ggtagaccgt ccaacggtgc aagccaatga agtttcaaag caggagctgc
    45181 acgcaggtgg gcgtaagctg attgaaaaaa ttgaagatta tcagccgcag gcgttggcga
    45241 ttctgggcaa acaagcatat gaacagggat tcagccagcg cggtgcacag tgggggaaac
    45301 aaacgctcac cattggttcg acgcagattt gggtgctgcc aaatcccagc ggtttaagtc
    45361 gcgtttcact agagaaactg gttgaagcgt atcgcgagct ggaccaggcg ctggtagtgc
    45421 gtgggcgata aaaaacgcca ccgaaaaggt ggcgtttgtg caaagggact agtgccgggt
    45481 gcggcgtaac gcctgatccg gcctaccgat taatcgtcca ggaagctacg caacacttca
    45541 gaacggctcg ggtgacgcag tttgcgcagc gccttcgctt cgatctgacg gatacgttcg
    45601 cgggtaacgt cgaactgttt acccacttct tccagcgtgt ggtcggtgtt catatcgata
    45661 ccgaaacgca tacgcagaac tttcgcttca cgcgcggtca ggccagccag cacgtcgtgc
    45721 gttgccgcac gcaggctttc ggtggtcgca gaatccagcg gcagctcgag ggtggtatcc
    45781 tcgatgaaat cccccagatg cgaatcttca tcatcaccga tcggcgtttc catggagatt
    45841 ggctctttgg cgatcttcag cactttgcgg atcttgtctt ccggcatcag catacgttca
    45901 gccagttctt ccggcgtcgg ttcacggccc atctcttgca gcatctggcg agaaatacgg
    45961 ttgagtttgt tgatagtctc aatcatatgc accggaatac ggatggtgcg cgcctgatcc
    46021 gcgatagaac gggtgatcgc ctgacggatc caccaggttg cgtaggtgga gaacttgtaa
    46081 ccacggcggt attcgaattt atcaaccgct ttcatcagac cgatgttgcc ttcctgaatc
    46141 aggtcaagga actgcaagcc acggttggtg tatttcttgg cgatagaaat aaccagacgt
    46201 aagttcgctt caaccatctc tttcttcgca cggcgggctt tcgcttcacc gatggacata
    46261 cgacggttga tatctttaac ctgctcgatg gtcaggccgg tttcttcttc aatctgctgc
    46321 agtttctgca gggcgcgatg cacttcttca gagacatcgt gcagtttttc cgaccacggc
    46381 ttgttcatcg caattgccgc gttgaaccag gtatcgctgg tttcgttgcc ggtaaacagg
    46441 gtaatgaagt ttttcttcgg cattttgcac tgctcaacgc agagcttcat gatcagacgt
    46501 tcttgcgtac gaacgcggtc catcatgacg cgcatgctgt tgaccaggta gtcaaactgc
    46561 ttcggcacca ggcggaactg tttgaatact tcagacagtt tcaggatctc ttcctgagcg
    46621 gcagcgtgac tgcgaccttt cgctttgatg gtgtcacgcg ttacaacgta ctgagcgcgt
    46681 agttccgcaa atttttcgcg agccagttcc gggtcgatgc tgttgtcatc atcggcgctg
    46741 tcgtcatcgc catcttcttc gtcttcatct tcgtcatcgt ccagatcttc ctgggaaagc
    46801 tcagaaccga cgtgagtggc ggtaggtgcc agatcttctt ctgcgttcgg gtcaacaaag
    46861 ccggtgatca gatcggacag acgcgcttct tctgcttcaa cacgatcgta ctgttccagc
    46921 agataggtga tcgcttccgg atattcagca acggagcatt gaacctggtt gatcccgtct
    46981 tcaatacgct tagcgatgtc aatttcgcct tcgcgggtca acagttcaac ggtgcccatt
    47041 tcacgcatgt acatgcgtac cgggtcagtc gtgcgcccga tttcagattc cacgctggaa
    47101 agcacctgcg cggcggcttc ggcagcatct tcgtccgcgg tgttttcagc cagcatcaga
    47161 tcatcggcat ccggtgcttc ttccatcacc tgaatgccca tgtcgttgat catttggatg
    47221 atgtcttcga tctgatctga atcgacgata tcttccggca gatggtcatt gacctcggca
    47281 taggtcagat agccttgctc cttaccacgg gtgacaagaa gtttcagctg tgactgcggg
    47341 ttttgctcca taagacggta tccacactta tttcatgagg ttggtgttgg gcgattgacc
    47401 cgatgtcctt cagccgttaa cgctgaaatc caacggttgt ttaccgccaa cgataattac
    47461 gagggcgtac ttatatattt gccgctgcct ctcagtgcgg ctgtcggggg cttcccgatc
    47521 gctcttcggc acttaagccg ttaaatcact ttttcgccag ctcctggttt aatgtccaga
    47581 gctccaggcg ttcttcgttg cttaaaccat gcgtgcgctc acgagcgatt aactcttcct
    47641 ggcgcagttc aagcagcgaa tcaaacatat ggttgagtga gtcggtgaag gtttgctcag
    47701 caatattctt atctgctata tcgtcccaca tcgacagttt ttcaagggtg gcagcattat
    47761 ttgtaccacg atagtgctct aaaagttgcc cggtggtcag acctggctgg gagagacaag
    47821 tgttgaccag ttctctgaat aagccaagtc cagggagctt attttcatcc agattctcaa
    47881 gcggcgggac caacgtcgct aattctggat tttgcaccag caaccctata agtatacgca
    47941 tggtcgtgcg ttttagctgc ggaacagggc gagaaacgcc gctctctgcc gcttttggca
    48001 ttaatcgttc aagctggctg tcatcaagta tgcctaattt gttgcctaat tcctgacgaa
    48061 gatatattcg cagcgtttcg cccggcactt gcgatatcaa tggcagtgcc agcgtactca
    48121 aacgtgcgcg cccgtcaggg gtactcagat caacttgcgg catcaggctg ttaaacagaa
    48181 atgcggaaag cggcatcgcc tgctccatcc gcgcttcaaa cgcttcttta ccctcttttc
    48241 gtaccagcgt gtcagggtct tcgccatccg gcaaaaacat aaagcgtagc tgacggccgt
    48301 ctgtcatgta aggcagcgcc gtttccagcg ctcgccatgc ggcatcgcgg cctgcacggt
    48361 cgccgtcata acagcaaatg acattgttgg tcgcgcggaa caacagttgt atgtgatcgg
    48421 cggtggttga cgtacctaac gacgcaacgg cgtaattaat gccgtattgc gccagcgcca
    48481 ccacgtccat atagccttcg accacaagca gacgattggg ttcagcgtta tcctgctgcg
    48541 cttcataaag accgtaaagc tggcggcctt tatggaaaat gtctgtttcc ggcgagttca
    48601 ggtatttggg ggtatcgttg cccagcacgc gcccgccaaa accaatcacc cgaccgcgtt
    48661 tatcgcgaat ggggaacatc acccgctcgc ggaaacgatc gtaactgcgt ccctgatcgt
    48721 tagtgaccaa catccccgca tcaatcaatg actggcgatt ttctggattg ccgccaaacc
    48781 gcttcaggac gttgtcccag ccggggggcg caaaaccaat cgcaaagcga gcgataacct
    48841 cgtggcttaa tccgcgtttt tccagatact ggcgcgcaga cgtggcaaca ggttgttgta
    48901 aagattgttg gtaaaacgta ttcagaccgt ccatcaactg ataaagcgtt tgcctctgat
    48961 ggcgctcgat ctggctgggg ccgctgcctg cttcaaatgg cacttcaaga ttgtgcattg
    49021 ctgccagctc ttcgaccgtt tcaacgaact cgagcttgtc gtagttcatc aggaagtcga
    49081 tcgcgttgcc gtgcgcgcca catccaaagc agtggtaaaa ctgtttctca ccgttaacgg
    49141 tgaatgacgg ggttttctcg ttgtggaatg gacaacacgc gtggaaattc ttgccctgct
    49201 ttttcagctt cacacgggca tcgatcagat cgacgatgtc agtgcgtgcc agcagatcat
    49261 taatgaatac gcgtgggatt cgtccagcca taggccccga tttttagcaa ttcataaacg
    49321 aaaataagcc gcgcattcct ttcggaagca cggccttaca actacaactc ggtctgatcg
    49381 gagagcaacg ctctcgggga attagtacag acgagtgcgg cgtgcgtttt cgcgagccag
    49441 tttcttcgcg tgacgtttca ctgcagaagc tttagcgcgc ttacgttcgg tagtcggttt
    49501 ttcatagaac tcacgacgac gaacttccgc cagaacacct gctttttcgc aggaacgctt
    49561 gaagcgacgc agagctacgt cgaacggctc gttttcacgt actttaatta ccggcatgtg
    49621 cctctcacct ttgattaatt cggtttgccg ctggcatcaa cgccagctta tttcaaaatg
    49681 gtgcggaatt ttactgcaat tgctgctgct ttgtaaagca ccgcggcctt tttgaaaggg
    49741 acttttataa gggtgaggag tatacacgag gctttctcca ggggcgaaca aagttttaca
    49801 tcaacccgca ttggtcctac actgcgcggt aataaagcga ggtaaaacaa gtcatgcgtg
    49861 tactgggtat tgaaacttcc tgcgatgaaa ccggcatcgc catttacgac gatgaaaaag
    49921 gtttgttagc caaccaattg tatagtcagg tgaaattgca cgctgactac ggcggcgtcg
    49981 tgcctgaact ggcctcccgc gatcacgtgc gcaaaaccgt accgttgatc caggcggcgc
    50041 taaaggagtc tggcttaacg gcaaaagaca ttgatgctgt ggcctatacc gcaggccctg
    50101 gattagtcgg cgcactgctg gttggcgcaa ccgtggggcg ttctctggcg tttgcctgga
    50161 acgttccggc aatcccggta caccatatgg aagggcatct gttagcgccg atgctggaag
    50221 ataacccgcc ggaatttccg tttgtcgcgc tgctggtgtc cggcggtcat acgcagttaa
    50281 tcagcgtgac tggcattggt cagtacgagc tgctcggcga gtctatcgat gatgccgccg
    50341 gtgaagcgtt tgataaaacc gcgaagctgc tggggctgga ttatcctggc ggaccgttac
    50401 tgtcgaaaat ggcggctcag ggtactgccg ggcgctttgt tttcccgcgt ccgatgaccg
    50461 accgtccggg gctggatttc agcttttctg gtctgaaaac ctttgcggcg aacacgattc
    50521 gtgacaacgg caccgacgac cagacgcgtg ctgatatcgc ccgcgccttt gaagatgcgg
    50581 tggtcgatac gttgatgatt aagtgtaagc gagcgttgga tcagacgggc tttaagcgac
    50641 tggtcatggc gggcggcgtg agtgctaacc gcacgttacg ggcgaagctg gcggaaatga
    50701 tgaaaaaacg ccgcggcgaa gtgttctacg cgcgtccgga attttgtact gataacggcg
    50761 cgatgatcgc ctatgccgga atggtgcggt ttaaagcagg cgcgacggcg gatctcggcg
    50821 ttagcgtgcg tccgcgctgg ccactggcag agttaccggc tgcgtaaaac tatccggtgc
    50881 cggagagcaa tttccggcac cgtcctcact taaagcaaca ccacgggcat ccacagcaag
    50941 ccggtaatca ccagcaacac gaggaatatc agtccaaaaa tcgcccccag tcgccagtaa
    51001 tccaccgttg gcagataacc actaccgtag taaatcggac tgggtccggt ggcgtatggt
    51061 gtaagaatgc tccccagacc aattgccgcg ccaaccatca ggcagaacac cggcagcggg
    51121 atttccggca tcgccagcgc agcggcaatc atcatcggtg cgagggcgga ggtatacgcc
    51181 gtggcgctgg cgaaaaagta gcgcagcaga taaaacacca caatcaacgc caccatcacc
    51241 atcgttggcg aataaccgct taagctgcct gctaacagtt tgccaaacca gctaataaaa
    51301 ccggtgttgt tgagtccggt agcgagggtg ataagcgagg ccagccagaa gaaaacgttc
    51361 cacgccgctt tattactgac aatgtcgtcc caactgataa tgcgcagaag cagcatcagt
    51421 gccaccacgc tgtaaccgac catcgcggca tcgatataat caccgccgaa aatccacagc
    51481 accagcgcgc ctaccatcag ccccagcatc cgtttttcac gcgaacagag cgggcccatt
    51541 gcctgcagtt ccgtctctgc ccagcgcggc acctgatcgc cagacttcag taccggcggg
    51601 tacagcacat aagccagcca gggaaccagc agcaccagca aaatactgag cggcaacatc
    51661 ccgaggaacc agtcgcccca actcagcgtg gcgtgagatg cgcttttcat cagtccaatt
    51721 aacagcaagt ttggtgccat cgccgtcaag aaaatggcgc tggtcacgca gtcggcaaca
    51781 atccccatcc acatgatgta cgagccaatg ctgcgcgaac tgctgtcgtt tggttgtgat
    51841 tgatagagcg gtggcaggtt acggatgatg ggatagataa tccccgcacc acgcgccgag
    51901 ttggacggtg ttacaggtgc gaggatcagc tcggagaaca tcaccgcata gccgagaaac
    51961 agcgtgcgat gtcccatctt tttcaccaga aacagcgcaa tgcggcgtcc aagcccggtt
    52021 ttttcatagc ctgtgccaaa cataaaggcg gcgaaaatca gccagataac cgaattagaa
    52081 aaaccggaaa ccgcccacga gagggatttt gcagtgaatt taaagcctgg ctgggcgagc
    52141 tgttcggggc tgaacagcag ccagggagag agtatggcga tgatggagat acccaccatc
    52201 gccaccacgg cacccggcac gggttcgagg atcagtccaa cgatcacgcc agtaaaaacg
    52261 gcaaagtaga gccaggtatg gttctccagc cctgcgggaa ccggaagtag agcaataatg
    52321 gcgatgaccg ccagcggcgc aagatatcgc caccattcag tggaaggttt catcgtgtta
    52381 tgcctgcgaa aaaggagagg ggtgagcctc tccgggaggg ttatttgatg taatggacgt
    52441 gctcgcagat ctcttccacg atgggatcgc ggcgttcggc gaacagcttt ttgttttcgg
    52501 ctatcaggtt gttgccgtgg gtatcaatag agacaatcag cgggccgaac tctttgaccc
    52561 ggcagaccca cagtgactcc ggcatcccga gttctgtcca gtgcacttct tcaatctctt
    52621 ctacctgggt tgccgccagc accgcgcagc ctgccgggaa aatcacatgt agcgccttga
    52681 atttctggca gccttcttcg gtcagcggcc ccataccacc tttgccaacc accagtttca
    52741 cgccggtctg ctcgataaat tcacgttcaa aactttccat acgcatactg gttgtcgggc
    52801 caacggaaac catctcccat ttatcgccgt ttttgcgcac gatggggcca gcgtggaaaa
    52861 tcgctttgcc gttgagatcg taagggatcg gacgtttcag ttcgatcaag cggcggtgac
    52921 aaacgtcacg gcaggtcacc agcgtaccgg tcaggtagat cacatcgccg acgcgaatat
    52981 cttgcagatc ttcagctttg atcggggttg tcaggatctt tttcataacg cgctccgggt
    53041 gtgagacaga ttttcaaagg tgagatcggc atgaaccagc agtgtgccgc gacgatgcgc
    53101 ccagcagcct gtagaaacag caacgccgat ggttgacgga tggcgggcgg cagattcgat
    53161 atgcacaccc atcactgaac tgttgccagt cagcccttgt gggccaattc ccagacggtt
    53221 gagtccttct tccaggcgta gctccagctc tgccgctttt ggattgggat ggcggctgcc
    53281 aatcgggcgc aaaatggctt ttcgcgagag cacggcggcg gtttccaccg aggtggcgat
    53341 gcccacgccc accagtaccg gtggacaggc gtttacggcg agggtggaga tattttcgaa
    53401 gacgaatttc actacgcctt cgtagccttc tgacggcatt aacactttcg agcggccagg
    53461 tagcgtgcag ccgccgcctg ccatgtaaac ttcgatttcc gcatcgtcat tgtcggggat
    53521 gatgtcccag gtgacccacg gtacgccgct accggtattt ttgccggtgt ttacttcgtc
    53581 aaaaatttct accgcattgt gacgtagtgg cgctttgacg gttgcctctt ccacggcttg
    53641 tttgagtatg ctttgcagct cgccaagcag tgggaagcgg gaaccgactt taacgaagaa
    53701 cataatctcc ccggtgtcct gacaggcagg acgattcagg tcaatcgctt tttgcatgtt
    53761 gtcgaacatc gtatggtaga taattttccc catcgacgac gtttcggcat cctttagctg
    53821 ttttagttta tccaccacgt catcaggcat tcgggtagaa atcatggcgg taaagttagc
    53881 gacaatctct gtcaacttat tcactgcctg ttgcttatta ctttcgctca tcatggcaac
    53941 tccagttttt gtatttgtct ggaaaaattc accgccacaa actaccacta aaaatgtggt
    54001 tttatatttt ttgccaccgt gaatatgtaa ttaacagttc gtgaatcaac gcgaaaataa
    54061 gaatgaatat tgcgccagag cagggaatgg caatgttttg cgaaatacac tgcgcttatc
    54121 acaactgcgg ataattaacg atgctgaata gctggccttt agccaaagac tt
//
LOCUS       DAFATA010000020        52379 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFATA010000020 DAFATA010000000
VERSION     DAFATA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.020"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..321
                     /locus_tag="LGJ20_001586"
     CDS             <1..321
                     /locus_tag="LGJ20_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="YdeF; uncharacterized member of the major
                     facilitator superfamily (MFS) of transporters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBK2385663.1"
                     /translation="GFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKA
                     SYFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQ
                     PALC"
     gene            448..543
                     /gene="mgtS"
                     /locus_tag="LGJ20_001587"
     CDS             448..543
                     /gene="mgtS"
                     /locus_tag="LGJ20_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBK2385664.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(762..1652)
                     /gene="dgcZ"
                     /locus_tag="LGJ20_001588"
     CDS             complement(762..1652)
                     /gene="dgcZ"
                     /locus_tag="LGJ20_001588"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBK2385665.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1907..2299)
                     /gene="ydeI"
                     /locus_tag="LGJ20_001589"
     CDS             complement(1907..2299)
                     /gene="ydeI"
                     /locus_tag="LGJ20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBK2385666.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2575..3093
                     /locus_tag="LGJ20_001590"
     CDS             2575..3093
                     /locus_tag="LGJ20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBK2385667.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(3138..5183)
                     /gene="dcp"
                     /locus_tag="LGJ20_001591"
     CDS             complement(3138..5183)
                     /gene="dcp"
                     /locus_tag="LGJ20_001591"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBK2385668.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            5320..6066
                     /gene="ydfG"
                     /locus_tag="LGJ20_001592"
     CDS             5320..6066
                     /gene="ydfG"
                     /locus_tag="LGJ20_001592"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBK2385669.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            6155..6841
                     /gene="ydfH"
                     /locus_tag="LGJ20_001593"
     CDS             6155..6841
                     /gene="ydfH"
                     /locus_tag="LGJ20_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBK2385670.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            7019..7222
                     /gene="ydfZ"
                     /locus_tag="LGJ20_001594"
     CDS             7019..7222
                     /gene="ydfZ"
                     /locus_tag="LGJ20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBK2385671.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(7258..8718)
                     /locus_tag="LGJ20_001595"
     CDS             complement(7258..8718)
                     /locus_tag="LGJ20_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK2385672.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(8807..10090)
                     /gene="ydfJ"
                     /locus_tag="LGJ20_001596"
                     /pseudo
     CDS             complement(8807..10090)
                     /gene="ydfJ"
                     /locus_tag="LGJ20_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            10807..11004
                     /locus_tag="LGJ20_001597"
     CDS             10807..11004
                     /locus_tag="LGJ20_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385673.1"
                     /translation="MDGESFNFDDNDIEFIASMYASCKLSGSIAPIMHIMVSIPRNKK
                     KQFYERVEHYCSLYSNKNPPS"
     gene            complement(11824..12405)
                     /locus_tag="LGJ20_001598"
     CDS             complement(11824..12405)
                     /locus_tag="LGJ20_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2385674.1"
                     /translation="MAFRMSEQPRTIKIYNLLVGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQAANEHIAPLQDAVDLEIATEEE
                     NSLLEAWKKYRVLLNRVNTTTAPDIEWPVAPIG"
     gene            complement(12405..15803)
                     /locus_tag="LGJ20_001599"
     CDS             complement(12405..15803)
                     /locus_tag="LGJ20_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBK2385675.1"
                     /translation="MAVRISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGIITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSVG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATATANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRRVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYLKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASVSSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(15868..16467)
                     /locus_tag="LGJ20_001600"
     CDS             complement(15868..16467)
                     /locus_tag="LGJ20_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBK2385676.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            complement(16535..20014)
                     /locus_tag="LGJ20_001601"
     CDS             complement(16535..20014)
                     /locus_tag="LGJ20_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBK2385677.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSDGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLITQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRPSDDASGYLDFFKGEIGKSHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            complement(20075..20677)
                     /locus_tag="LGJ20_001602"
     CDS             complement(20075..20677)
                     /locus_tag="LGJ20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBK2385678.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALAMQIPAFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPKTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(20614..21357)
                     /locus_tag="LGJ20_001603"
     CDS             complement(20614..21357)
                     /locus_tag="LGJ20_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2385679.1"
                     /translation="MTETESAILAHARRCAPAESCGVVVRTPEGERYFPCVNISGEPE
                     EYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASICV"
     gene            complement(21363..22061)
                     /locus_tag="LGJ20_001604"
     CDS             complement(21363..22061)
                     /locus_tag="LGJ20_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBK2385680.1"
                     /translation="MQDISQETHHETTRLTQSAQVVLWEIDLTVVGGERYFFCNEQND
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(22071..22400)
                     /locus_tag="LGJ20_001605"
     CDS             complement(22071..22400)
                     /locus_tag="LGJ20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2385681.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(22400..25465)
                     /locus_tag="LGJ20_001606"
     CDS             complement(22400..25465)
                     /locus_tag="LGJ20_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2385682.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAEQNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTREPRNDEATRLLL
                     QYSQQQAQVEGQIAAVRQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQNFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(25437..25766)
                     /locus_tag="LGJ20_001607"
     CDS             complement(25437..25766)
                     /locus_tag="LGJ20_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBK2385683.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(25775..26161)
                     /locus_tag="LGJ20_001608"
     CDS             complement(25775..26161)
                     /locus_tag="LGJ20_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBK2385684.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGVIENAGDLEHEVLTPEKS"
     gene            complement(26222..26965)
                     /locus_tag="LGJ20_001609"
     CDS             complement(26222..26965)
                     /locus_tag="LGJ20_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2385685.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVAAVAVSAAG"
     gene            complement(26977..27378)
                     /locus_tag="LGJ20_001610"
     CDS             complement(26977..27378)
                     /locus_tag="LGJ20_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2385686.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(27375..27953)
                     /locus_tag="LGJ20_001611"
     CDS             complement(27375..27953)
                     /locus_tag="LGJ20_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2385687.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKVPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(27965..28240)
                     /locus_tag="LGJ20_001612"
     CDS             complement(27965..28240)
                     /locus_tag="LGJ20_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HBK2385688.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(28233..28556)
                     /locus_tag="LGJ20_001613"
     CDS             complement(28233..28556)
                     /locus_tag="LGJ20_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBK2385689.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(28643..30670)
                     /locus_tag="LGJ20_001614"
     CDS             complement(28643..30670)
                     /locus_tag="LGJ20_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK2385690.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQMLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSGYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSFTRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(30615..32123)
                     /locus_tag="LGJ20_001615"
     CDS             complement(30615..32123)
                     /locus_tag="LGJ20_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2385691.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYETDGNDSKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            complement(32123..32335)
                     /locus_tag="LGJ20_001616"
     CDS             complement(32123..32335)
                     /locus_tag="LGJ20_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385692.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(32332..34431)
                     /locus_tag="LGJ20_001617"
     CDS             complement(32332..34431)
                     /locus_tag="LGJ20_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBK2385693.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            complement(34440..34979)
                     /locus_tag="LGJ20_001618"
     CDS             complement(34440..34979)
                     /locus_tag="LGJ20_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBK2385694.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATVRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            complement(35529..35735)
                     /locus_tag="LGJ20_001619"
     CDS             complement(35529..35735)
                     /locus_tag="LGJ20_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385695.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            complement(36031..36203)
                     /locus_tag="LGJ20_001620"
                     /pseudo
     CDS             complement(36031..36203)
                     /locus_tag="LGJ20_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
     gene            complement(36376..36531)
                     /locus_tag="LGJ20_001621"
     CDS             complement(36376..36531)
                     /locus_tag="LGJ20_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385696.1"
                     /translation="MREYPNGETTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAMRLLN"
     gene            complement(36679..36867)
                     /locus_tag="LGJ20_001622"
     CDS             complement(36679..36867)
                     /locus_tag="LGJ20_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK2385697.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSHLSLDLRK"
     gene            complement(36878..37090)
                     /gene="cspI"
                     /locus_tag="LGJ20_001623"
     CDS             complement(36878..37090)
                     /gene="cspI"
                     /locus_tag="LGJ20_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HBK2385698.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            complement(37454..37951)
                     /locus_tag="LGJ20_001624"
     CDS             complement(37454..37951)
                     /locus_tag="LGJ20_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HBK2385699.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAVRIIEQGRSISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYESLRD
                     KKYQM"
     gene            complement(37948..38481)
                     /locus_tag="LGJ20_001625"
     CDS             complement(37948..38481)
                     /locus_tag="LGJ20_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBK2385700.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(38478..38789)
                     /locus_tag="LGJ20_001626"
     CDS             complement(38478..38789)
                     /locus_tag="LGJ20_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HBK2385701.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEAAARMSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            complement(38794..39009)
                     /gene="essD"
                     /locus_tag="LGJ20_001627"
     CDS             complement(38794..39009)
                     /gene="essD"
                     /locus_tag="LGJ20_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBK2385702.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            complement(39261..39635)
                     /locus_tag="LGJ20_001628"
     CDS             complement(39261..39635)
                     /locus_tag="LGJ20_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HBK2385703.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(39807..40235)
                     /gene="tolA"
                     /locus_tag="LGJ20_001629"
     CDS             complement(39807..40235)
                     /gene="tolA"
                     /locus_tag="LGJ20_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBK2385704.1"
                     /translation="MGFRSASILTLIISGIIIGCTDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
     gene            complement(40602..40733)
                     /locus_tag="LGJ20_001630"
     CDS             complement(40602..40733)
                     /locus_tag="LGJ20_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBK2385705.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(41233..41309)
                     /locus_tag="LGJ20_001631"
     tRNA            complement(41233..41309)
                     /locus_tag="LGJ20_001631"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41273..41275),aa:Arg,seq:tct)
     gene            complement(41410..41485)
                     /locus_tag="LGJ20_001632"
     tRNA            complement(41410..41485)
                     /locus_tag="LGJ20_001632"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41450..41452),aa:Ile,seq:cat)
     gene            complement(41629..42450)
                     /locus_tag="LGJ20_001633"
     CDS             complement(41629..42450)
                     /locus_tag="LGJ20_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBK2385706.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPLVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(42447..42821)
                     /locus_tag="LGJ20_001634"
     CDS             complement(42447..42821)
                     /locus_tag="LGJ20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBK2385707.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMNEEQVKK"
     gene            complement(42834..43883)
                     /locus_tag="LGJ20_001635"
     CDS             complement(42834..43883)
                     /locus_tag="LGJ20_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBK2385708.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQLADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(43885..44163)
                     /locus_tag="LGJ20_001636"
     CDS             complement(43885..44163)
                     /locus_tag="LGJ20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385709.1"
                     /translation="MARNVKYYNSDNSPVLACTHERYSHAFKSEWFLHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(44230..44481)
                     /locus_tag="LGJ20_001637"
     CDS             complement(44230..44481)
                     /locus_tag="LGJ20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385710.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            complement(44698..44853)
                     /gene="hokD"
                     /locus_tag="LGJ20_001638"
     CDS             complement(44698..44853)
                     /gene="hokD"
                     /locus_tag="LGJ20_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBK2385711.1"
                     /translation="MKQQKAMLIALIVICLTVIVTVLVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(45536..46468)
                     /locus_tag="LGJ20_001639"
     CDS             complement(45536..46468)
                     /locus_tag="LGJ20_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385712.1"
                     /translation="MEINKEKLSGLLNYFESRKKYAATRISSLSSQNEICFALSGLTF
                     RNGPYVFQDVGELREVLHPPGEIELAKALKSKSLLSAVARHMPAVTHELALQSSESKF
                     SQSDLTIGWWIISAIRCRTLTDILVPAVASASWDVFPALQANSCEVQLLEDVPAVRRI
                     SPSVEISVESLDWVQENLKRWIDLLEQPAFRLAIDSLTTHNQHANLRMAAAALWAGFE
                     ALFGINSELRFRLALMAAAYLEERGPDRLATYRRVKKLYDYRSKAVHGGATSDNFLVE
                     HIIEVRVLLSRLVCRMTEIGKMPTTDDYEERLLT"
     gene            complement(46658..47059)
                     /locus_tag="LGJ20_001640"
     CDS             complement(46658..47059)
                     /locus_tag="LGJ20_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBK2385713.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKTAVIDPDLIWSLPDG
                     EIRRYDSRLNIICRECRMSEVMQRILAFYQG"
     gene            complement(47100..48065)
                     /locus_tag="LGJ20_001641"
     CDS             complement(47100..48065)
                     /locus_tag="LGJ20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385714.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSSVQRCVNHISEPDTEIPPGFRG"
     gene            complement(48046..48567)
                     /locus_tag="LGJ20_001642"
     CDS             complement(48046..48567)
                     /locus_tag="LGJ20_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385715.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(48551..48778)
                     /locus_tag="LGJ20_001643"
     CDS             complement(48551..48778)
                     /locus_tag="LGJ20_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2385716.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            48856..49263
                     /locus_tag="LGJ20_001644"
     CDS             48856..49263
                     /locus_tag="LGJ20_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2385717.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            49456..49611
                     /locus_tag="LGJ20_001645"
     CDS             49456..49611
                     /locus_tag="LGJ20_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBK2385718.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            49613..50188
                     /locus_tag="LGJ20_001646"
     CDS             49613..50188
                     /locus_tag="LGJ20_001646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385719.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRTNKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            50675..50863
                     /gene="dicB"
                     /locus_tag="LGJ20_001647"
                     /gene_synonym="ftsT"
     CDS             50675..50863
                     /gene="dicB"
                     /locus_tag="LGJ20_001647"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBK2385720.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            50860..51051
                     /gene="ydfD"
                     /locus_tag="LGJ20_001648"
     CDS             50860..51051
                     /gene="ydfD"
                     /locus_tag="LGJ20_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HBK2385721.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            51144..>52379
                     /locus_tag="LGJ20_001649"
     CDS             51144..>52379
                     /locus_tag="LGJ20_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK2385722.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDNLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARA
                     RANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLD"
BASE COUNT        13071 a        14158 c        11668 g        13482 t
ORIGIN      
        1 ggttttattt tttccggcaa cagcctgcta ttgtggggta tgtcagctgc ggtatttact
       61 gtcggtgaaa tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca
      121 ggaatgaaag ccagctattt ttccgcccag tctttaggct ggcttggtgc cgcgattaac
      181 ccattagtga gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt tgtcatctta
      241 gcgttggtga tcattgctgc gtgggtgctg atgttaaaag ggattcgtgc aagaccgtgg
      301 gggcagcccg cgctttgttg atttaagtcg aacacaataa aaatttaatt cagccttcgt
      361 ttaggttacc tctgctaata tctttctcat tgagatgaaa attaaggtaa gcgaggaaac
      421 acaccacacc ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta
      481 ctgggaataa ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac
      541 taatgaacgg agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc
      601 ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga
      661 tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg
      721 acacagtagc atcagttttc tcaatgaatg ttaaacggag cttaaactcg gttaatcaca
      781 ttttgttcat caataaacat gcagcgattt cttccggttt gcttaccctc atacattgcc
      841 cggtctgctc ttccaatgac cacatccaga ggctcttcag gaaatgcgcg actgacacct
      901 gctgtcacgg taatgttgat atgcccttca gaatgcgtga tggcatggtt atcgactaac
      961 tggcaaattc tgacacctgc acgacatgct tcttcatcat tagccgcttt gacaataatg
     1021 ataaattctt cgcccccgta gcgataaacc gtttcgtaat cacgcgtcca actggctaag
     1081 taagttgcca gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc
     1141 aatttaaatc ggtcaatatc caacaacatt aaataaagat tcagaggctc agcgttgcgt
     1201 aactgatgat caaaggattc atcaagaacc cgacgccccg gcaatcccgt caaaacatcc
     1261 atattgctac ggatcgtcag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa
     1321 agcaacccct cctgaaaggc gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc
     1381 agcattaatt cccgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg
     1441 taaggtaatt catcgttatc gagtggcccc agatgatcaa tccaccgacc aaactggcac
     1501 agtccataag aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg
     1561 tgaaacatac tcaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag
     1621 atggcatcaa tttccgttgt tttcttgatc attgccactc ctttttcaca gttccttgtg
     1681 cgcgctattc taacgagaga aaagcaaaat tacgtcaata ttttcataga aatccgaagt
     1741 tatgagtcat ctctgagata acattgtgat ttaaaacaaa atcagcggat aaaaaagtgt
     1801 ttaattctgt aaattacctc tgcattatcg taaataaaag gatgacaaat agcataaccc
     1861 aataccctaa tggcccagta gttcaggcca tcaggctaat ttatttttat ttctgcaaat
     1921 gagtgacccg aacgacggcc ggcgcgcttt tcttatccag actgccgcta atgttgatca
     1981 tctggtccgg ctgaacttct cgtccatcaa agacggccgc aggaataacg acattaattt
     2041 caccgctctt atcgcgaaaa acgtaacggt cctctccttt gtgagaaatc aaattaccgc
     2101 gtagtgaaac cgaagcgcca tcgtgcatgg tatttgcgaa atcaacggtc attttttttg
     2161 catcatcggt tccgcgatag ccatcttcta ttgcatgagg cggcggtggc gctgcatcct
     2221 gttttaaacc gccctggtca tctgccaacg cataaggcat gacaagaaaa cttgctaata
     2281 caatggcctg aaatttcata ctgactcctt aattgcgttt ggtttgactt attaagtctg
     2341 gttgctattt ttataattgc caaataagaa tactgccaat tgttataagg catttaaaat
     2401 caggcaacta gctgtcaaat attcagagaa tttaactcac taaagttaag aagatcaaat
     2461 agtcttaaat atattttcag aataatcgga tttatatgtt tgaaaattat tatattggac
     2521 gagcatacag aaaaagcaaa tcacctttac atataaaagc gtggacaaaa aacagtgaac
     2581 attaatagcg ataaaattgt acaacttgca gataccgaca ctattgaaaa cctgacatcc
     2641 gcgttgagtc aaagacttat cgcggatcaa ttacgcttaa ctaccgccga atcatgcacc
     2701 ggcggtaagt tggctagcgc cctgtgtgca gctgaagata cacccaaatt ttacggtgca
     2761 ggctttgtta ctttcaccga tcaggcaaag atgaaaatcc tcagcgtaag ccagcaatct
     2821 cttgaacgat attctgcggt gagtgagaaa gtggcagcag aaatggcaac cggtgccata
     2881 gagcgtgcgg atgctgatgt cagtattgcc attaccggct acggcggacc ggagggcggt
     2941 gaagatggta cgccagcggg taccgtctgg ttcgcgtggc atattaaagg ccagacctat
     3001 actgcggtta tgcattttgc tggcgactgc gaaacggtat tagctttagc ggtgaggttt
     3061 gccctcgccc agctgctgca attactgcta taaccaggct ggcctggcga tatctccagg
     3121 acagccattg gtggtgttta tatgttcaag ccacgatgtt gcagcatcgg cataatctga
     3181 ggagccttac cgcgccattg tcgatacagg cgttccagat cttcgctgtt acctctggaa
     3241 aggatcgcct cgcgaaaacg cagcccattt tcacgcgtta atccgccctg ctcaacaaac
     3301 cactgataac catcatcggc caacatttgt gtccacagat aagcgtaata acctgcggca
     3361 tatccgccac caaaaatatg ggcgaaataa ctgctgcgat agcgtggcgg tatagcagga
     3421 agatccatat tttccgccac cagcgcccgc aattcaaaat catcgacatc ctgcattgct
     3481 tcgttttctt ccaggcaatg ccagcgcata tcgagaagtg cggcgctaag cagttcgctc
     3541 atctcatacc ctttgttgaa caggctggca ttacgcattt tctgttgcag ttcgtcaggc
     3601 attgctgccc cgctctgata atgccgggcg tagcgagcga atacctgcgg atgcgttgcc
     3661 cagtgttcgt tgatttgcga cggaaattcg acaaaatcac gcggcgtgtt ggtgccggaa
     3721 agcgtggcat aacgctggcg ggcaaaaagg ccgtgcagcg tatgaccaaa ttcatggaat
     3781 aaggttatga catcatccca gagtaacaac gcaggctcac cggcagcggg tttctgataa
     3841 ttgcaaacgt tataaattac cggatgcgtt tcattaagcg ttgattgctc aacaaaattg
     3901 cccatccatg caccgccgct ttttgaatca cgggcgaaga aatcaccgta aaataacgcc
     3961 agtcccacgc cattatgatc aaaaatttcc cacacacgaa cgtcaggatg gtagacagga
     4021 atatcaaaac gttcgacaaa cttaataccg aagagctgat tcgcggtcca gaatacacct
     4081 tcattcaaca ccgtgtttaa ttcaaaatat ggcttgagct gcgcctcatc aagatcaaat
     4141 ttctcccgcc gtacctgttc ggcataaaat gcccagtccc acggctgcgc gctaaacccg
     4201 ccctgctgct tatcaataac cgcctgtatg gaggctaatt catcgctcgc acgttgacgc
     4261 gccgctggaa caatttcccg cataaagttg agcgctgctt caggtgtttt tgccatctga
     4321 tcggcgattt tccatgcggc ataatgagga aaaccaagta atgtcgcctg ttgtgcacgg
     4381 atctccacca gacgttgaat gatagcgcgg gtatcattgc catcattttt ttccgctcgc
     4441 gtccagcccg caataaacag tttttcacgc gtcgcacgat cgcgcatttc ggcaagcgcc
     4501 ggctgttggg tggtattcag cagcggaatc agccatttgt tatccagacc tttctcgcga
     4561 gccgcctctg ccgccagcgc aatctcttgc tcactcattc ctgccagctg cgcgatatcg
     4621 ttcacaacca gaccgccgga tttatttgct gccagtaacc gctggttaaa ctgactggtc
     4681 agtgtcgcag cttctgtatt cagtactttt aattttgctt tatcagcttg cgcaagtttg
     4741 gctccggcaa ggacaaaacg ttgatgaatc acctccacca ggcggatgga ttcactatca
     4801 agccccaggg attcacggcg ctgccagaca gcatctaccc gcgcgaataa ttcaccgttc
     4861 agatagatat cattagccag ttccgccagt tcagcggaaa actgttcatc aagacgctgt
     4921 aattcatcat tggtatgcgc cgcagtcatc gcaaaaaaga cgctggtaac gcgggtaagt
     4981 aattctccgc tttgttccag tgccagaata gtattgttga aatcaggcgt ttgcgggtta
     5041 agcgcgatgg cagcaatttc tgcccgcttt tgctgcattc cctcatcgaa tgccgggcga
     5101 tagtgatgat tggcaatttg atcaaaatga ggagccagat acggcagtgt gctttgcaca
     5161 aggaaaggat tcattgttgt cattttcttc tcctgaacgc gaggtgttcc atagcgtagg
     5221 cttactgata acggagtgca atcttgcaat ccagtattac ccgctcttaa gcatcccgtg
     5281 ctatgttatt gacacacaaa agcgttgagg aacagtgaga tgatcgtttt agtaactgga
     5341 gcaacggcag gttttggtga atgcattact cgtcgtttta ttcaacaagg gcataaagtt
     5401 atcgccactg gccgtcgtca ggaacggttg caggagttaa aagacgaact gggagataat
     5461 ctgtatatcg cccaactgga cgttcgcaac cgcgccgcta ttgaagagat gctggcatcg
     5521 ctccctgccg agtggagcaa tattgatatc ctggtaaata atgccggcct ggcgttgggc
     5581 atggagcctg cgcataaagc cagcgttgaa gactgggaaa cgatgattga taccaacaat
     5641 aaaggcctgg tatatatgac gcgcgccgtc ttaccgggta tggttgaacg taatcatggt
     5701 catattatta acattggctc aacggcaggt agctggcctt atgccggtgg taacgtttac
     5761 ggtgcgacga aagcgtttgt tcgtcagttt agcctgaatc tgcgtacgga cctgcatggt
     5821 acggcggtgc gcgtcaccga catcgaaccg ggtctggtgg gtggcaccga gttttccaat
     5881 gtccgcttta aaggcgatga cggtaaagcg gaaaaaacct atcaaaatac cgttgcattg
     5941 acgccagaag atgtcagcga agccgtctgg tgggtgtcaa cgctgcctgc tcacgtcaat
     6001 atcaataccc tggaaatgat gccggttacc caaagctatg ccggactgaa tgtccaccgt
     6061 cagtaatttt tatacccggc gtaactgccg ggttattgct tgtcacaaaa aagtggtaga
     6121 ctcatgcagt taactcactc acaagcaaga acgaatgacc gtcgaaacgc aacttaatcc
     6181 cacacagcct gtcaatcagc agatttatcg tattcttcgt cgcgacattg tccattgcct
     6241 gattgcacca ggcacaccgt tgtcggaaaa agaagtttct gttcgtttca atgtgtcacg
     6301 ccagccggtt cgtgaagcct ttattaaact ggcggaaaac ggcctgattc aaattcgtcc
     6361 gcagcgtggc agctacgtca acaaaatttc catggcccag gtgcgaaacg gcagttttat
     6421 ccgtcaggcc attgagtgcg cggtggcgcg tcgggcggcg agcatgatta ccgaaagcca
     6481 gtgctaccaa ctggaacaaa atcttcacca gcaacgcatt gccattgagc gcaagcaact
     6541 ggatgatttt tttgaacttg atgacaactt ccatcaactc ctgacgcaga ttgccgactg
     6601 tcaactggcg tgggatacca ttgagaacct gaaagcgacc gttgatcgcg tgcgctatat
     6661 gagtttcgac catgtttctc caccagaaat gctgttacgc cagcatcttg atattttctc
     6721 tgccctgcaa aaacgtgatg gcgatgcggt agaacgtgca atgacgcaac atttgcagga
     6781 aatcagcgaa tccgtgcgcc agatccgcca ggaaaacagc gactggttta gcgaagagta
     6841 attcatttac tctcatccca tccgggatga gaggcttttc cccccgcctt acggctcatg
     6901 catgcatcaa aaaagatgtg agcttgatca aaaacaaaaa atatttcact cgacaggagt
     6961 atttatattg cgcccgttac gtgggcttcg actgtaaatc agaaaggaga aaacacctat
     7021 gacgacctac gatcgtaacc gtaacgcaat caccactggc agccgtgtta tggttagcgg
     7081 caccggtcac actggcaaga tcctgtcgat tgatactgaa ggtctgaccg ctgagcaaat
     7141 ccgccgcgga aaaaccgtag ttgttgaagg ttgtgaagag aaactggcac ctctggacct
     7201 gattcgtctc ggcatgaact aagtgtgtga atgccgccga tggcggcatt gcttttttta
     7261 cttcacggaa tatttcgcca cggtcgcttt cgcgccatgc gctaataaag acaagtacgc
     7321 ttccgtcact tttgcagtaa acaagctatt gtctggcaaa tcatcaccaa agattgcctt
     7381 aatcgccagc aatgactgga cgcgcgcttt cccttcggca ctactttgta cagccttctg
     7441 aataacaggt aacagtgggt cactgatttc tatcggattt ccctgttcat caacaccacc
     7501 gacataacgc atccaacccg cgacgcccag cgccagcaga tcgaacttgc tgtcatgcgc
     7561 cagatgccag cgaacagaat ccaacatccg ctgtggcaat ttctggctac catccatcgc
     7621 aatctgccag gttcgatgac gtaacgccgg gttgctatag cgtgcaatta atcggttagc
     7681 gtaatcttgc aaatcaacgc cctgcacttt cagcgtcggc gcttgttcct gcaacatcaa
     7741 gccatacgcc gcataacgat aatgttcatc ttccatgcag tcattaatgt gctgatatcc
     7801 agcaagataa cccaaatacg ccaggaatga atgactgccg ttgagcatgc gcaacttcat
     7861 ctcttcataa ggcagcacat cactaaccag ttcggctccc gctttttccc attctggacg
     7921 tccggcaaca aagttatctt ctattaccca ctggcggaaa ggttcacagg caacgcccgc
     7981 aggatcgccc acaccggtaa gttgttcgat tttcgccagc gtatcctctg tcactgcggg
     8041 cacaatacgg tccaccattg ttgatgggaa agtcacgtta tcttcgatcc attgtgccag
     8101 tttttcatca acggctttcg cgtatgaagt gacaacgtca cgcataacat gaccgttttc
     8161 tggcatgttg tcacatgaca tgacggtaaa tgcgggaagt cctgccgctt tacggcgggc
     8221 cagcgcctca acaatcaccc ctgttgctgt tttcggctgg tggggatttt gcacgtcggc
     8281 agcgaccatc gggtgatcga gcattaactg tccggtcgcc ggagagtgga aatacccttt
     8341 ttcggtgatt gtcagagaga caatcgcgat ttgcggttca cacatcgcag ccaacacggt
     8401 ttctaagcca tccatctgta cgtgcaaggc ttttttaacg acgccaacga cgcgagccgt
     8461 ccacgcatcg gccgacattt ccgcaacggt ataaagatta tcttgctgtt gtaaatcggc
     8521 aatttgctgt tcgccgccga ttaagttgac ctcataatat ccccagtcac tgaaatgttc
     8581 tgtagcaaga atatcggcat acacgccctg atgcgcacgg tgaaatgcac caaagcctaa
     8641 atgaataatt cttggggcca ggttattacg atcataaaca gggagtgtcg cttttgctga
     8701 taacaaatta tttcccataa caattcctta aatttaaata tggcaagcta tatgttttgt
     8761 tatatgaata aaaatcccct ctccggtaag agaagggatt gagggtttac agacttctgg
     8821 aaggttgcgc agctcttaca acacgcggtt gatcttccgc agcgccttcc agcgcactta
     8881 aatcacggtc tttcacctct ggcattttca gcgcagagat taaaccaatt actgaatatg
     8941 ccatgatcat aatggcgatc ggataccagg attccgtcat ggtgcagaaa atacccgcca
     9001 ggataggacc aaaaccggaa gcgataagac caccaatttc tttagaaata gccatccggg
     9061 taaagcggtt tttacagccg aacatttctg ccatggtaat gttttccaga gcaaataatc
     9121 ccagcaccgc acagttatga atcacaatca gtgcaaccat aatggtgctc ggggcatagc
     9181 ttttatctac aatgatagaa agcattggcc atgccagcac aatcgcggag gtattcataa
     9241 taatatacgg gatccggcga ccaattttat cggataacca accaaggaac ggaatggtca
     9301 taaagccgag aatcgaacta atcatcaatg catctgttgg aattgctttg ttaaacaata
     9361 acgtctgcac taaatagcct gcaaggaaag tctgaattaa cccggagtta cccgcctgac
     9421 caaaacgcag ccctgttgcc agccagaagg atttgctctg gaacatgcta ccagcaggtg
     9481 caggttttgc tgtgggttgg ttgctgtcat taaccttctc aaagaccggg ctttctttca
     9541 gattcatacg taaccagata gcaaagacca tcacgacaac gctcgccagg aacggtatac
     9601 gccatcccca cgccagcagt tcctctttac tgagaatgaa gaacataaag gcccagattg
     9661 ccgttgcgct caaggttccg cagttagttc ccatagctac aaatgaggag ataattccgc
     9721 gcttaccttt tggtgcatat tccgccagca tcgtaccggc accggaaatt tccgcacctg
     9781 cacccaaccc ctgaataata cgcagcgtca ccagcaagat gggggcaaaa acaccaatct
     9841 gtgcataggt cggtaacaca ccaattaagg tggtacagat ccccatcatg gtgatggtaa
     9901 taaagagcac ttttttacgc cctattctgt cgcccatttt gccgaaaata aatgctccga
     9961 caatacgcgc cacataacct gcaccgtagg ttcccattgc caaaattaac gccattgccg
    10021 ttgatgattc aggaaaaaat atttcatgaa acactaacgc tgcgccgagc gaatataact
    10081 ggaaatccat aattcacaga tgttttttct cattctgtgg tttccttggc gctttctagg
    10141 ttttttcaga tagttacatt ttttcaaaag catcctgagt tcgatcttac tatctatctg
    10201 tggcctattt ctgtcccata tatgccccaa aaaacacccc aacagataag cagtcttttc
    10261 atggtgttac gcgtaaaaac aataacggct tgaaattatt caatacactc tcgaaagtgc
    10321 atcagccaac cgcagaacgt tattgcatac aacgtttctg cggcatatca cgaaacgatt
    10381 acttaatgac tgggacttca gaccactcaa tatccggtac aattatgtat caacgcagtt
    10441 taacaacacc ctatatttaa tccagagtgc gagcaacgtt gtccctttct cttttgcact
    10501 cttaaaatat acgattactt aagtgaagat gcttgacgaa tcgccaaata aaacatcttc
    10561 atcagcataa aaatccatat atgattgaaa atttagtcat taataccatt catatctgaa
    10621 tactgaccta atatgcttac taaccgtgag aaatcttttt tttcgactta atcactcgaa
    10681 ataaactttc actcaattga aaacaattag ctacaattat cattgcaata tcaaatcaaa
    10741 cataacaact tgcataaatt ctcatgggat acagcatcag ttgtgccaaa cacataagga
    10801 attgaaatgg acggagaaag ctttaacttt gatgacaatg atattgaatt tattgcttca
    10861 atgtatgcat catgtaaatt atcaggaagt atcgccccca taatgcatat aatggttagc
    10921 attccaagaa ataagaaaaa acaattctac gaacgagttg agcattattg tagtttatat
    10981 tccaataaaa acccaccttc ttaaattcgc tgcattctag tggattccat taagaaaaca
    11041 atgacataca ctcaaacaca tcttttttat caacagtgaa tttcagttcg gccagttttt
    11101 accagctatt ataaaaatag catccatata aagtttttac cccacaggta cagctggcca
    11161 ttcgataacc gcactaagga agtgccaaca cgatatcagc aagacccgat atttcttcca
    11221 tgccgccagc aacgatgtta aggttaaatt aattataaaa aaatatatta taattccatc
    11281 ttcgcattaa attttaacct ttaatctcca cacggaacaa gttgcatagg tgactatatt
    11341 aggaaaactt ataaccacca aaacatttgc tttgcaaaca aaaacagtga ccattgactc
    11401 taagaaaatt ccgatctatt gttagggtca tcaaagttac ttaacagtat tccgaaatga
    11461 aaatacaaag caagctaatt gtcttattaa acattatcat tgcatttata ttatttgagc
    11521 ttttagctca aaaatttggt ttgagtacat tattttaaag caagataaac aatatcgggc
    11581 actctagttt ttataattaa caattaaacg tccataaaat gttatttact tttgttcgct
    11641 attgtcatat cttaatatag tggcttacgc cactctactt tcatcacatt ttgtacgttt
    11701 atgtactcgc catgaagctc tttcatggcg attttttatt cttaatccaa gaacatactc
    11761 cctacctata gcaaaatcac tttttgaaca aaagaataac gtatttaggt aatcattacg
    11821 aatttaccct ataggtgcta ctggccattc aatatccggt gcagttgttg tattaacacg
    11881 gttcagcaac acccgatact ttttccaggc ttccagcaac gagttttctt cctccgttgc
    11941 gatttccaga tctacagcat cctgaagtgg cgcaatatgc tcattcgctg cctgcatcag
    12001 gctgtttttt gtttcttccg cctcccggat ccggaatagt ttttctgctt ctgcatcttt
    12061 cacccaggct gtgccgttcc acttctgaaa ctccccttcc ggggataacc aggtgacatt
    12121 ttccggtaat gagccgagtt cagaaataaa taacgcgtcg ccggaagcca cgtcataaac
    12181 cgttttaccc cgatggtctt caacgagatg ccacgatgcc tcatcactgt tgaaaacagc
    12241 cacaaagcca gcaggaatat ctggtggtgc aatatcggta ctgtttgctg gcagacctgt
    12301 atgaggcgga atatatgcgt caccttcacc aataaattca ttagttccga ccagcagatt
    12361 ataaattttt atggtccgtg gttgttcact cattctgaat gccattatgc aagcctcaca
    12421 atatagttaa atgcgatgtt tttgacggtg ttttccgcat taccagcagc gttaacggtg
    12481 atggtgtgtc catgtgaacc aatcgcaacg gagtgcgtat gagcaccaat accgacagta
    12541 tgcgcgtgtg cacctgcaga tgctgctgtg ccggacagtg agtggctatg attaccatct
    12601 gtactggtat tagctaacca ccccgtagac atacctactg aaccttgtac accccaggta
    12661 ttttgacctg agcttgtata accatattga taagtatctt taaaaacact ggggttaaat
    12721 cgacggccat ctctatggct gtgattacca gctgcattcg tgctgccact taaactatga
    12781 gtatgcgcac cagtgttatt cgtggattta gtgccgtaat caaacgatga tgtggttttc
    12841 gtacccaaat ccgtactgga tacgctggcg ctgtgggtgt gcgatttaat gccgtcctgt
    12901 tcctgagaca atacggcacg accactggcg ggcttgccct taatcgtcca gccacgcata
    12961 tcagggatca cgcctgacgg ataagcggcc gcaagtttca ggtaagcaga tttgtcaaaa
    13021 gtctgcccct gcatcagggc ataaccagac ggaacggtat ctgatggcca cgggattggt
    13081 gcgccgactg ggtagctttc tggtggaaga tttttcgagg tataaacttc tgcccagtct
    13141 tcctcaaaac cataaccgtc tcttgaagaa cggtagaaca gaccaccatt tctgtaatgc
    13201 gccttcatct gcagggtccg gcaacttccg actccggtat agaagttaac cagaatatag
    13261 ctgtcgccag agcgggtgac attataagcg cctgattcgg cattccaggg aacgccacca
    13321 tccgcatcgg catatgtatc cgttgccctt ctggcaaaag cagccacatg cgcggcggtt
    13381 aaagtaatat ctttggaacc atcaaactca acaccagaaa cccgtcttgg cgtttgcagc
    13441 tttgttgctg ttaatgcatt accgttcaga cttgcggaca gtttggttcc aataaccagt
    13501 tcgccggttg cgttatcaat agcaaacggt cttaatgtat tccagccacc ataaacatca
    13561 ccttgattgg taagcagcag gtaagtttta gcgccatcat tacgccataa tgccccatac
    13621 tccccaccta tcattcgaat ctgattacca ccacgcgcta caatttcgtc tgtggcaaaa
    13681 agttttttgc acgacaagtt atcgttaacg attaacgaat gagactcata aaaaccacgc
    13741 ccactcttaa aatcaaggat aacgtccgcc gcgatacatt cagtcgccgg atttgttgcc
    13801 ccaaacttat aggtcgtatc attaacaacg agatcagcac caggtgcgga tattgacagg
    13861 ccatcttcga taaacgcaaa aacagggaaa gcagcgccat caacatagaa cacagagcgc
    13921 aaatcatcgc ccttattact catcattatt gagtggatgg ctcgttcatt gttttgatat
    13981 tgccagaaca ttccataagc ataacgcccc ctgtcagtcc agccaccagg cataacaaat
    14041 ccgttaaact cgcagttatt catcggatcg cctgcggttc gcgttgccgt ggtgataatg
    14101 acccttgatg ccagttcgct tactgagcca gcagaacgca taacaacaac agggtaatat
    14161 tttccagatg ttgcacctgc aggagcgtta acccgcacat aacgcatacc acgcttatca
    14221 gcaaagtctg ttttactgac cgcgttaatg ttgttcagga agcgtccctt atcgggtata
    14281 tcagcgccgt tctggtcttt ctgcagacgt ttctctgcat tgtcataggc tgattttact
    14341 gcctttggcg ttgccgccag cgtttcagac gtactgttgg tcgcactgct gagctgtact
    14401 atcccctttt tcgtcgtact tgcatcctca agcgccacgg cggatgcaat atcctctgcc
    14461 cgttttgccg ctgtctcggc gcgcgttgcc gcggattctg ccgtactttt gctctgtgct
    14521 gccgccgtcg cactgccagc agcctctgtc gccttcgtgg atgccgtcgt ggcgctgccc
    14581 ttcgctgctg acgcttgtct ggtcgcctca tcttttgaag cagacgccga tgatgccgat
    14641 gacgccgccg aactggctga cgatgcggca gccgtttttg aggattctgc gctggtttcc
    14701 gacgctttcg cgttcgtttc ggatgtcttc gctgcggaag cagaactcgc tgctgcgctg
    14761 gcctgttcag tggcttcgcc agccttcgtt gtggctgttg aagcagacga tgcggcactt
    14821 tctgccgatt ttccggcggc ggtggcactg gctgaggcct gcccggcact tgttgacgcg
    14881 gcactggcag acgacgcagc cgctgttttt gagcctgccg cagccgaggc gctctgtccc
    14941 gctgccgttt cagaagacct ggcgttcgtc tctgacgttt ttgccgcctt cgcggaattt
    15001 gctgtcgccg ttgccgagga agctgcgctg ctggcgctcg aggctgcgct cgtttctgat
    15061 gattttgccg cctcttttga agctgacgca tcccgggctg aggtggcagc ttctgacgct
    15121 ttcgtggtcg cggtggatgc agaagtggct gctgattgtt gtgacgctgc cgcattcgtt
    15181 tctgacgttt tcgcggcact ggcactggta gctgccgcgc tttttgagga ctctgcagca
    15241 gcagcacttt tcgatgcttc agtggccttt gttgatgccg ttcctgcgct ggaagacgct
    15301 gactgagccg acgaagcggc ctgtccgact gacgtgctgg ctgcgcgtgc tgagcctgca
    15361 gcatcagtcg catgggttgc cgcctcacgg gcagatgtgc cggcatcgcc ggctgacttc
    15421 ttcgcggctg ccgtgttctg tgccactgcg gacgcgttac gcgccacctc ttccaccatc
    15481 agttcaaaac ggcgcagtgc ctctggccgg acatcatcct ccgtcattgc accgaggaaa
    15541 tcattcagtg tgcccggctt tgagtcctca tatacggtga taatcccggc gtgtgacggc
    15601 gggaatccct ccaccaacag actgacgctg tactgaccat actcgacgtc cattgtgtaa
    15661 cgcccggctt catccgggtt ttctgaggcc actgtgttca ccaccaccgt ggtgctgttg
    15721 cgcctggcct ttagctgaat ggtgcagttt tgtatcggct tacctgcgcc atctttcagt
    15781 acacctgaaa tccgtactgc catattcccc ccacaaaaaa gcccgcctga accggcgggc
    15841 tgtcataaca ctgtgttacc tggctaatca gaatttataa ccgacaccaa cgatgaaact
    15901 gttggtacgc cagtcaccgc tgccggagcc ttcataagcg acgtcaacgg ccacggattc
    15961 ggtcgggtta aactgcacgc cagctcccca cgccagagac gtgttgctgt ggcgaccgtc
    16021 atcacttccg gtcagcacat cgtgcgtttt ccccttgttg tcagttacgc ggatataatc
    16081 cccggagaaa gtcgacacac ggctgtaagc catacccgcc atcgcatacg cgctgaacca
    16141 ttcattcacg cgcacagacg gccccgccat cacgctgaac cagcgattac gcacggaatc
    16201 ctcatgccag cgggtatcgc tgtaatgcgt tttttgctca tcttcagcgt tggcataact
    16261 gaatgacgta atcagcccca gcgtgtccgt aaactcataa cggtatttca cgttaatccc
    16321 gttcagatta tcgctgccgg gagcgttcgt acgggcatga agataccctg cgctcagtgt
    16381 ggcctgctgc tcagacgccc atgcaggcgc accggatacg gccagacaga tggctgcgga
    16441 caaaatggct gcacaaactt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc
    16501 gccatttctg gcgcccgtat atgggttata aaattcagct gatactgatg cctgcggtgg
    16561 ctttcttcat caccacaacc agcaaatcgc tgatacttgc tgtgggatac cagtcattta
    16621 ccagccatgc tgacaccgaa aactccagcg tcatgtggcc gtgaccagct ggcatatcaa
    16681 taacaccact gtaaatcagc gtattatcca gcgcggtacg gttataaatt tcagcaccat
    16741 ttttcttcac tatcaggcgg catgaggagt aaatatcagt atgctctctc tcatgtttag
    16801 caccgctgaa tgccaccgcc ggaataacaa tctgccggtc aaacggctga tcgtcataaa
    16861 tcctgacggt aatggtccct gatggccacc gctccggtgc ccgggagtcc cgcgggaaag
    16921 ccttacccac tgttttgaca atatcgcctt caatctggtt ggctgacagt ttccctttaa
    16981 tctgacagtt ctcatttatc gtaacgttgt tgagcgtccc ggagttcgca ttcacactgc
    17041 cactgatatc tgcattttta gcggtcagct ttccgtccgg tgtcagggaa aatgcaggag
    17101 gattaccgcc gctggtaatg gtgggagccg tcagatattt caggaacact tcattcatga
    17161 atatctgatc accctgacca acaaacatcg gttttgtgtt gccatttgca ggattaatca
    17221 tcgcaatcct gtctgccgcc agcagcacct gactctgcat tcctgctggc gtattctcaa
    17281 taccggcacc gatacccgcg atataaaggc gtccgtcctg catctgctgc agcttcacag
    17341 cccacatgct gttcaggtta ttatttgtat caacctgaac cttctgtatc tgctggatcg
    17401 ctgcactctg atcttccagt ttcttattga cggtctgtgt gatttcattg ctgacatccg
    17461 taatggacgt cctgatttca gccaggtcag gcgcaagctg accgttatca atctgcgtcc
    17521 acaactcctg agccagatgg cttttcccta tctcgccttt gaaaaaatcc agatagccgg
    17581 aagcatcatc actcggccga ccaacagcct ccacgaatgc cgatttgcca acggtattca
    17641 cactgcgaac gtaaaaataa taatcatggc ccggcctgat attgatactg gcggctatcc
    17701 agtacagcgc cgtaccaaga taacgcgcgc tggtttcaac ctgcctgata tccgcaatcc
    17761 gcttttccga gaaccagaac tcaaactgta ccgtcggatc ataaaccgca agatgcggcg
    17821 tggcggttat ctgaaaatac ccgggtgtca gctcaatctg cgacggcgct gccggtgcgg
    17881 caatccggaa cgataccgat gccggatcgc cctgctgtcc ccacgcattt accgcccgga
    17941 ccgtcagcgt gtaacgcccc agcgccagct gcctgaagcg gtatgtggtt tctgtcgtcc
    18001 gggccgtgct gaccagccgc tcactgccgt cgtccgctgt tacggtcagg cgaagcataa
    18061 agctcacccc cttcaccacc ttcggcgtgt cccagcgcgc cagcacctga tattccccgc
    18121 tgtctgcggt gacttcggca gtcaggtgct gcactgctgg cggcgtgaca ccattcaccg
    18181 tgccgctctg gtcgccgtca aagtgcgccc cgttatccac gatggcttct ttttccggta
    18241 catgctgcac ggcggtgatg gcatacgtgc cgtcgtcgtt ctcacggata ctcacgcagc
    18301 ggaacaggcg ctggcgcagc gtcggcagct tcagccccca cacgctgtat ccggcaacgc
    18361 cgtcaggaac acggctcact tttaccttca cgccgtcggt gacggactgg acctccacgc
    18421 tgaccggatt gccacttccg tcaaccaggc ttatcagcgt ggtaccggag gatggcagcg
    18481 tgatttcacg gtcgagcgtc agcgtccggg tctggctgtt caccgccagc acgcgcccgc
    18541 cggtgctgat ccccgcatag tcatcatcac agatttcaat gacatcacct ggtacatggc
    18601 gaagcccttc ggcacccacg ctgaagtcca cggtctgcgt ttccagcagt tccgttttaa
    18661 tcagccacag cccggcgcgg tgtgcctgcc cccggctggt acagccaaag gcatccatct
    18721 tcgtgacgtt acgaccgtaa cgggcaatgg cctgcgtgtc ctccacaagc tctgtcgccg
    18781 tctcccagcc gttatccggg tcaatccagt tcacctcaac ggcattatga cggtccttca
    18841 gggcactgaa gctgtagcgg aacggcgcgc catcatccgg catcaccaca ttactgcggt
    18901 tataggtcca caccttatcc gacggtcggt cctgcacgaa cgtcagcgtc tgcccgttcc
    18961 ataccggcat acagcgcatc gccgagcaga aatcactgag aacatcccac gccttgcgct
    19021 gtgtgatcag ccaggcatta caggtgatgc gcggctccgt gccaccaaag ccatccggca
    19081 ccgactggtc gcaattctgg ccgatgacat acagcgccca tttgtccaca tccgccgcac
    19141 cgagacgctt ccccatgccg tagcgcggat gggtcagcat atcccacaga caccaggcca
    19201 tgttgttgct gtatgctggc ttaaacgttc cgtcccagat accgctgtat tgccgcgtct
    19261 gcgggttata gttcgacggc acctgcagaa tgcgcccgcg aagatgataa ttacggctca
    19321 cctgctggct gccgaactgt tccgagtcca cctgcacgcc gaccagtgcc gtgttcgggt
    19381 agcactgttt cacatcgatg atttcggtgt atgacgacca gagcgttttg ttctgcagct
    19441 ggtctgtggt gctgtccggc gtcattctgc gcatccggat attaaacggg cgcggcggca
    19501 ggttatccac caccaccgag gccagatact gcgaggtggt tttgccctta atggtgatgt
    19561 ctttttccgt cacccagcca ccattacgct gtatctgaac cagcaggcga acttccgacg
    19621 gattccggtc cccctttgag gtggtttcca ccagtgcctg cacaccgaag gtaaagcgca
    19681 gtcggtcgat gtttgccgac gtgatggcgc gggtgatcgg cgtgtcgtat ttcacttccg
    19741 tacccaacac cgtctcggag ccggaggatt caaatccctc cggcggagtc tgctcctgct
    19801 caccagcccg gaacaccacc gtgacaccgg cgatgttggt attcccatca ctgtccagca
    19861 ccggcgtact gttcagcagc acgcttttta atccatccac cggaccttca atcggccctt
    19921 cactgatggc atcaatcaca ctcagcaact gcgtggactt caggttgtcc ttcgcttcgc
    19981 gcggggtatg ccccttactg cttcctttac ccattcctca cgctccataa acgacaaaac
    20041 cgcccgcagg cggtttcaca taaaacattt tgcatcagcg accaatcacc acaacctgac
    20101 caccatcccc ttcgtctgcc gtgctgatct cctgagaaac cacgcgtgac cccacgcgca
    20161 tttcaccgta cagaacaggc agaacattgc cctgggcaac catgttatcc agtgaagaaa
    20221 aataggtgtt ctgtttgccg ttatccgttg tctgtgtgcg gggggttttg ggtttagggg
    20281 ccagcatctg tgcaacaccg ccaagcgtca tactggcacc gagagaaaac agcagattac
    20341 tcgccataat tcctaccccc ggcatccata tagcaaccgc cataacagcc gcccccagca
    20401 cagcctgaaa cacaccgcca cttttggctc ccgccagacg cggcacgata tggatcacag
    20461 caccatttgc cagcggttca ttaagacggg ctgataattc cgtttcacct gtatcacgcc
    20521 cggcaatgcg tacctgatac cagccgtcgc tcagcttctg acgaaacgcc gggatctgca
    20581 tggccagcgc ccggatggct tcggcccccg ttttcacacg cagatcgatg cggcggccaa
    20641 atcgttgtaa atccccgtaa aggcagatgc gcgccatgcc cggtgacgcc agagggagtg
    20701 tgtgcgtcgc tgccatttgt cggtatacct ctctcgtttg ctcagttgtt caggaatatg
    20761 gtgcagcagc tcgccgtcac cacagtaaat ggcggcatga ttcggcaccg atgaaccaaa
    20821 gcagcacagc agcacatcgc ccggttgtgc tgatgacaac ggcacctgat acagccctgt
    20881 ggcctccaga ttatccagat agagattctg accgtgacgc caccagtcat ccccgcgatg
    20941 aaaatccggc atctcaattc ccgccagatg gtaagcgtcc cggaacagcg tgtaacagtc
    21001 cgtcaccccg tgctcaaagc gccgcccggt gagatgcggc acacagcgga atttatgaat
    21061 cgccccccgg cagaccagcc accacggcaa atcactctgc acctgcagcc gccggtcggc
    21121 ctcactcagc cagggcagac caccggggtg gctgtggacc agcgccacaa tctctccctg
    21181 catttctgca ctcagccagt cctccggcga catccggaaa tactcctccg gctcaccgga
    21241 tatattcacg cagggaaaat atctttcccc ctccggtgtt ctcaccacga cgccgcacga
    21301 ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct gattctgtct ctgtcatggg
    21361 attcactgcg aaagtttgtt aatggaaagg aagccgccaa agttgccgac gttattgcga
    21421 aacttacagc cgctcaggca tttgctgcat ttatccttcg tgatatcgga cgtcggctga
    21481 tcatattcat ccgcgaccgc cggaccgtga taaccgcact catcaccgcg ataggtccag
    21541 gtgcaggtgt tggccagcat gatacgtccc ggaaaaacgg caccgtccgt ttccgtcggc
    21601 gtggacagta caaaagaggc actcaccgcg ctcagttcac tgcactgctc gatgcgccag
    21661 cggctgatca cctcctgctc cggatcggcg tcactgtttc cgttgacgaa gttcaccgca
    21721 tccagaaaac gggcgtaaac cttacgccgg accaccgttc ctccgaccag actctgcagg
    21781 tcttccgcca tcccggtgac catgccgtgc aggttagaaa ccgtcagtgt cggacgggca
    21841 gcactgcctt tgccgttcag ttcaaatccc gtcccctgaa tgggatacgc ctgatactgt
    21901 cgcccctgcc aggtgaccgg ctcaccttta tcgttctgct cattacagaa aaaataacgt
    21961 tcaccaccga ccactgtcag atcgatttcc cagagcacca cctgggctga ctgagtgagg
    22021 cgtgtcgtct catgatgtgt ttcctgtgaa atatcctgca tcagggcctc ctatgccacg
    22081 acctgttcaa aatctgccgt tatggttacc cacagcgccc ccacgcttgc cgaccattta
    22141 cgacaaacca ccctgatcgg cttccagtca taaggtggcg tccactgaaa tgcgcggacg
    22201 ccaccgtgcc gttccagaaa ggcttttaaa gatgggtgtt cacatttacg aacacgtatc
    22261 gtcacgctgt aaatcgacaa ctggttattc agtcccgccg cacgacgctg ttcataacca
    22321 tcgcccagct tcactgtcac cactttcggc tctgatacca cattcatatc cgggcgcact
    22381 ttccagtgaa acgtctccat taccgatatg ctccacttaa ccgaccacca tcacgggcct
    22441 gctgttgcat aaagtccgct gccgcttttt tcccgaggtc ataaaccacc ttcagggcag
    22501 ccggacctat ctgcccgttc gtgccatcgt tattgatctc gatgttgtac tgcggggcaa
    22561 acatcgccat acctgaacca ccaatatccg ccacaacccc cagcttaccg tcagcaccac
    22621 gacgcagtgg cagaatggct tcaggtcccg cttcccccat cacacccgcg ccttttgcaa
    22681 aagcaaaaaa cgtcggacgg ttaaccaccg tgccactgta acgactcaaa tcagcagact
    22741 gataaatccc cccattggca ttcagggaaa ggctgctgag atcaaatccc agtacactgc
    22801 caatcccttt tatcgatttc atcatggttg cctgcgccag gattttcgcc atgtctgaca
    22861 gcacagaaga ggtgaaggat ttgaaattga gtttgccggt agtgacaaaa gttgccaggc
    22921 catttcccat gttgctgaag gctgacgaga acacctgttc tgctgttcct gccgtattat
    22981 ctgcatccac ggtaaaattc tgaaatgcac gcaggactcc gtttttccag ttaccctgca
    23041 caacttcaag ctgttgccag taacggcgat tctcattcag ttgtcggttc aggctctccg
    23101 tcagcgcctg ctcggccttt ctgtagtcat ccgtgttata tgttcctttc tgctcactat
    23161 cccgcctcaa ctgctccagc tgttgctggt atttctggcg aagactcagt tgtacctgat
    23221 atcgctgccg ctgctgatca cccataccca ccgtggcgat atccaggtca tgttgctgac
    23281 gctgagcgcg ctcttcttca gccagttgac tggtcagctg aattgttttt ttcttcagct
    23341 cgttgagtgc cgtctgtttc tgaagctcct gctgttttac atccagcagc gtcagtgcct
    23401 gaatcagttc atctttacgg gccagcacac tcttttcatc tgccgtcagt tttttcccgt
    23461 ccaggtcgct gatgcgctgc tgcagagcca gaagctgttt atgcgcttct gtcatccttt
    23521 ccgtggcaat gcctgctgac tgtctgacag cagcaatctg tccttccacc tgtgcctgtt
    23581 gctgactgta ctgcagcaat aaccgggtgg cctcatcatt acggggttcg cgtgtttttt
    23641 tcttaccgga tgccagggct ttctcgtaac gttcattttc acgttgtatc gccgcatccc
    23701 tgacagcctg atcggcgtac tgcatggaat taatacgcgc aatttcacgc tgatgtcgtg
    23761 ctgcttccgt ttcattcatc cggttcagtg cagcattttc agcattacgg cgtttctgtt
    23821 gctcctgata attctgctct gcctgctctt ttgcatcctg caaatccttc tggcgttttt
    23881 tctcctgaag ctcgttaaga cgctgctgat cgtactcaac ctgagaagat gatgccgtcc
    23941 aggggagtct tttcgcccgc gacactttct cctgtaaagc ggaaatctgt tcatccagcg
    24001 agtcttcacg accaatattc atggccgcat cccagaaacg actccataaa tcagacagat
    24061 acttcagcgt actgcccagc gcattgaggt tattatcaat atccgcagta cgccgaccgg
    24121 tttcctctgc cagtgcagac atggctatcc gtgccgcatc actggaccgc ccctgttccc
    24181 caaggacgcg tatctgctca agctgagtgg cagtaagaaa atgcagctca ttgtccagag
    24241 ccttcgcggc atttacagga tcatccttca gccgcttaaa ctgatttatg gtatcgctga
    24301 ccgactggcc aaccgatcgc tccatctgtg cggcagctct cgccaccata ccgatatcgt
    24361 ttccacgaaa tgcaccactc cccaccacct gagccagcgc accggctgca gcatgttgcg
    24421 tgataccatt cccggaaata gcacgactga gcgtccacag ctgcccggca gtgactccgg
    24481 cataatgccc cgtcagcgac aactggcggt taaattcttc cccctccttc tgaccatcat
    24541 accaggcttt acccagacca tagacggccg cggcaatacc gccaataacc ccgcccagca
    24601 tcatgccttt cggtgacatc agtgtgtcta tccatccggc acggttagcc agcgttatcc
    24661 cggatcccct cagcgcacct aaattgccgc gagccagttc acctatcaga acgcctatct
    24721 cctggcgggc cgctgcactt ttcagaccca gcgaatgcgt ggcttttcct gcctgctcca
    24781 ttttgcggat atacacttct gcagcactgc ttacccccag ctgggcagcc ttagcacgaa
    24841 gcaactcaga agaagaaaga ttctggcggg ttgcctgctc tttaagctga cggataaacg
    24901 ccactttctg tcgggtagcc tcttcctcag cctgtgtaag aacacgagtt ttcgcagtaa
    24961 cctcagaaat cagcgccaga taatcctgct gaccaatccc gccactgttt ctggcctgtc
    25021 ggatctgctg ctgaatacgc tgtaattcct gcagccccgc actggcctgt ttcacactgt
    25081 caatctgacg ataaaacgca gcagccgctt tatcctgagc ctccgccaga gccatggcct
    25141 gcgcctgttc ctcgcgcatt ttctggctca gtgcctccat gcgctggcgg gttttctcca
    25201 cctcgcgggc catgcgttca tgagcctgtg cgttcttctc caccgtctgc gcatggacgg
    25261 atgcggctgt tgcagccgaa gaagctgcct gcgttgtctg ccgggcggcc tgagtctgac
    25321 gctccataaa acgctgcata cgggcagaag accgttctgc atcgctggct gcaccattca
    25381 gaaggttttt gatacgggga atttcatttt taaactctgc cgcatcaatc cccaaatcaa
    25441 tgaccaggtt ggctatctgg tccataacgc acacctccgg aaataccttc cccaagatgc
    25501 atcagttctt cgtccgttcg ctccggtatc ccgttctctt ccggtaaaag gctgaaatca
    25561 gccaccgcag catcactgct accggacacc attctcacga tcaatgcctt cagcgaggca
    25621 aactgcgcat ccatccacac atcactgaag ctctgcatcc ggaaataatc gccccactca
    25681 ccaagctcag tggccgacat ttccgacagc atccgccgcc agtctgcccg ccggaactcc
    25741 cgggcaagcc gcatgacaaa ctgcatttcc cgcgtcagga cttttccggc gtcagcacct
    25801 catgctccaa atccccggca ttctcaatga ctcccatacc gctcagcgac agaaccatct
    25861 ccgcccccgc tcccagggca tcatacgacc atgttgtaat aacggatgcg caaagcgtct
    25921 caacatcctg agactgttcc gcattccaca gtgagcggga aaccagccag gcattgatat
    25981 ccatccccat ccgcagaaaa gcaatctgtc gttcagcctc cggcagttct ccctcttcgg
    26041 catcaaactt tgccgttcgc tgctgaacaa acgccagata ttcaattctc tgcagcccgg
    26101 acagctcact gagcaccacg gactgctttt cataattaaa cgtgccctgt ttcagaaaca
    26161 tcatgttctc cacctgcaaa aaagccccgg ataaccgggg caaatgatga gtatcgtcct
    26221 gttaacctgc ggcgctgaca gccaccgcag ccactgccac aaaatcgccg tcagaagtca
    26281 tgcccacaat gctgacactg ccctgcttca cgcctttcac cgtggccaca agcccgttca
    26341 gggtcaccgt ggcagtctgt ggatctgtcg aatgcacact gatcgctttg tcactggctc
    26401 cgtcaggttt tactgtaaag gtcagcgtgg tggttgctcc cacttttaca ctggcagatg
    26461 ccggtgccac cgtcagcccg gtaacgctca ctgtttcagt gccttcctct gccagatacg
    26521 gacgccccac accgctgatt ttcactgtgc gggtcatcac gtcttttgag gcaatggttt
    26581 tacccagtga gctcagccag ccacggaaaa catcaacagt gccgttggga tatttgatac
    26641 gaaacgcgca gacttcaccg gagtcgaaca actgaaccag ttttttctgc ccgctgtcac
    26701 ccggacgcca ggccagcgtc gccgaagtat caccaacgga tttctgcccc tgggttgtcg
    26761 ttttccagtc tgcatcttca tcatcgagat aagtgtcatc ttctgcatca gcggtcattt
    26821 cgccaggttg cagatccttc accatcgcaa gacgcagcca gtcagtgtcc gataaagggt
    26881 tcgcaaacgc atcgcccttg ccggtgtaca tccagaacgt cgttcccgca cctttcgttt
    26941 ttgccagtgg atttggtgtg gtcattgcca cctcctttaa ttcgtgtacg tgatctggta
    27001 cgtgatttcc gccatcgccc aggtagccat ctcattatca cgttgatagt taaaaccgag
    27061 agggatcagg gtgtcgatga gtccggaaag tgccggtata tcattcaggg ccgggaaaat
    27121 ggtgctctcc atccacatat ccagctctga atccggtgcc tgtgcccgga tgaagacagc
    27181 aatatgcaga acagcctgcc agtcatcttc atccgtcatt tttccggtgt actgagcatc
    27241 actcagccac accgccacgg caggcagttc ctgcgcatca ataaatgccg gaagcccgtc
    27301 aaaaaacgtg gcgctgtctc cacactgttc ccgaaggcgt gccagtacgg cctggcggat
    27361 ttgtgtatgt cggttcatcg ggtcagccat aacctcagtt gttgtttcag tgcatacccc
    27421 agctgtttcg gcatttccgc agcaataatg cggtcgcggg catcttcaaa tgcctgtgtc
    27481 agcggtccgg acagcgggac tttcaccaca tcaatggggt aacgattttt gccatcaata
    27541 cgccgcatca catgccagcg accattcgcc agttgctgaa taaacgcatc ccggaaaaga
    27601 tatttaccca ccttcagcac actgccacgg tactgcagtt ttccaccacg ccgggccagt
    27661 ctgacccggg ctgtccccag cttaatagcg ggcagattgc cccggttaac gcggatcctg
    27721 gccgtcattt ttcctgacgg actggcttta aacacccgga cacgctgacg taccagtttc
    27781 agggggatcc ctttcacctg gttatctccc gcaacggtat tcccggcaac ctgccgggtg
    27841 gcgaccgaga ccgctttctg tgccacacgg tttatcgccc atgcgctggc ctgtggcacc
    27901 atacgggtat caaggctgtt cagattacgg atggcattct caagcccctt catcccacac
    27961 ctctttactc aataaagatc attggcttac cgttaaaacg ttcatgccgt gtgaccgtcc
    28021 attgttgtcc gtcataaaca acgcgatccc cgcgccgtgg gcggtatccc gaagaaaaca
    28081 ccaccagaga gaccgcaggt ccggacagag cattcagctc tgccagtgtt tctcccggga
    28141 tcacagtcat atcgacatca ttaatcgagg ctgtctttcc catctttcta accgtgatag
    28201 catccatacg cgctgccagc cgggaaaagg gatcagacat tgagttttac cggcacttct
    28261 tctgcactgg ttccggcatc tgcccagaca accccgacca gcggatcaga gccgctgtta
    28321 gtcagctgaa cttttccgga cttcagataa accttcttac ccgttttcat gtcatccgtt
    28381 ttcagcttag gcagcataaa cacaccttcg gtcatgccgt cgcctgtttc accctgtgga
    28441 atatcggtca gcgccaccgc aaaaacatcg cccacctgca ccagatctcc gctgctgatg
    28501 gctgcactgg caacaatcgc caccgttttt ccttcttcta caaaattctt tgccataact
    28561 gtctccgcac agccccgttc aggggctgat ttcaggtaca aaaaaagccc ttacgggcca
    28621 tcagagttgt tgtctgcgac gtttacgccg tacatttcac cagaccgcgg tgatcaactg
    28681 gcgcgacacc ggcgtcaata cgcactttcg ttgtcacgcc atccacactg aagccctcca
    28741 tctgatcaat atatggcgta tccacaccgt tgagataagc cacttcaatc gtatcggagc
    28801 cttttgacgc agccaggtag aaggtggtct ggctgttatc atcaagacga ggctctgcaa
    28861 taacggtcgc aaaatctttc accgggttaa taataccggc gttaatgtca gcccccttga
    28921 cacttgagga gcgaatgacc tggttagcaa cagactccat cgccgtcggt accagtacga
    28981 acgcaggacg aatattcaga tgacgctccc cctctttctg aacgcgcatc aactggcggg
    29041 ctttatccag cgatgccacg tccattgcag cgctctccag tacgtttgca tgtttcgctt
    29101 tatcgaacag acttacatta tctgtggaga ttttcgggtt agacgtcaga atggcataaa
    29161 ccagatcggc aatagtggat ttcgccgcac ggcccagttt catcgggaca tcggtcagca
    29221 tattcagatc atcattgata atggcctgac gggtgaaact gaacagctcg ccataggtcg
    29281 ccagtgcaat agtggcctgt ttatctccgg tggtgacgta tttatattcc gccccttcac
    29341 gcacctgacg cagagcactg aagcccccca tacccacacg atgggcaatt ttaaaatcag
    29401 acaactgacc tttccgcgtc cactgttcat aggtttcagg ggcatcttcc cagccctgca
    29461 gaatggcttt gttcgcaaca tccagcagaa tattaccgaa gtcagacgta ctgtgtgtga
    29521 acgccgcacc gaccatctgc atcgggttat aaccggaaac cccaataccc cgttcagtta
    29581 gtgacatacg ggcatattca cgcagggtca tcccgttgta gacattatca cgttcggttt
    29641 tttcaaatcc ggcacgcgcc atcagcgcct ggcggatccc gtcccccaca aaattaccgt
    29701 taccggcata aatatgagcc ggggtatttt tattggatgg cgtggactcg cgccccatct
    29761 cgttcaacag cttttcacgg gcctgctcca gcgaacattc aggatcggca agacactggg
    29821 cctgcagcat ctgataacgc ccgccaaaca tggcaaacag atcattaata ccgtttacac
    29881 gcgctttttg ctctgccagt acctgcgcac ggatactgtt ttcatccacc acgggtgctg
    29941 ctgcctgcac tggcgtccgg gaggctgcag gttcatcatc ctgtacgcgt ggagcactgt
    30001 tgcgtggcgg agtaatcatg tttcgaatgg attccggcat ctttttaaat tcctctgtac
    30061 gttttgactg aatacatgcc attgccttaa cggctggtgt cacctgatca gcaaatccct
    30121 gtgccagaca ttcggcaccg gacatccagg tctcatccgc cagcatggca gcaatttcat
    30181 cggtggtttt cccggttttc tgtgcataag cgggtaacag aaccgcctca actttatcga
    30241 gcaggtcggc ataggtgcgc atgtcctccg catcaccgcc cgtaaagcca aatggtttat
    30301 gaatcatcat gaaggagttt tccggcataa tgaccgggtt tcccaccatc gcaatgaccg
    30361 acgccattga cgccgccaca ccgtcgacat aaacggtaat ggacgcacca tgtgttttca
    30421 gtgcattaaa aatggcgatg ccttcaaaga catcgccacc cggtgaatta atatggagat
    30481 taatgtgggt gatatcgccc agtgcattca gatcactgat aaactgcttc gctgtaacac
    30541 cccagaaacc aatctcgtca taaatataaa tatccgcgtc actctggtga cctgcctgca
    30601 tcctgaacca ggaattattc ttcggactgg tcgtcggtgt gctgcggctc ctgtcgtttc
    30661 gttgcggcac tgctgcctcc tttatcactg gccggatcgg tatcaaatac cagatccagc
    30721 ttgcggtttt catcaatttc ggccttgcgc cgacgtttga catcatccgg attacgacca
    30781 cctgcacgta cccagtctga ttctgtcgcc gctccaccac gaatctgaat tttccaggcc
    30841 tcagcctcct taacagggtc aatccacggc atcaccggtc cggaatacac cgcggtatac
    30901 agtgaagatc ggtccagatc gcggggtagc ctgataacac cggatgccac agcctgtttc
    30961 agccaggcac ggtacatcgg gcgggtgacg gcaccaataa accagtcctg caggatcagg
    31021 tagccatcag tagattcaac cagctcctga cgctgggcgc tgtaagtgcc gttatagttg
    31081 cgcgctgtac tggaaaaact cagacgactg cccgcagcca cggcacgcaa ctgaccatta
    31141 cgaaaagttt caaggttagg attggggcga tccgacttca ccattccgat ttcttcgccg
    31201 ggtttcagat cgtcgtaaat aatgcctggc tgaatggtaa gctcgcgttc attctccttg
    31261 ctgtcattac catccgtttc atagctctgt ccgtcgcctt tccggatgta catccccaga
    31321 gcagcggcga tccttgctgc agtcagctca gaatcttcat actctttcag agcactgagg
    31381 cggatcagca caccggacaa caaagacgtc ccgcgcatct ggtgcagacg gcgaacaaat
    31441 ttaagatgca gcattcgctc tgcatccact tctttggttt ccatctgccg cccggatacg
    31501 ggacggcttt tatacaccag atatttttcg ggacgccccc agtcatcaac aaacacgccc
    31561 tgattcagcc tgttgctctc atcactggtc atgggaataa agtccggctc gagcgcctcc
    31621 agccagaaat gaacaccggc agaaggcgtc aagctgttta tgcgcccgga aaccatctgg
    31681 gcaaacacct caccatcgcg cagccaggta cgcagcatca gacgttccag catcggacgg
    31741 gtaaactgcc cggtgacttc cggactgaca gaccattcac tccatcgggt gcgtatctcc
    31801 gctgccagat cacgggcaat ggccccattg cgtaataccg gatgtggctc gacaataatc
    31861 ccgtttttcc ccaccacccg ttcttccagc ttgtcaaata caccaatgac cagatcgtgg
    31921 ttgttatcaa ggtaacgggc ctgctcacgt aacgacacgg ccccgtactg gcttaactgg
    31981 tcggcagttc ggttctcccg tcgggctttg tgtgtccgcg tcgtttttac ggcctcataa
    32041 gcctgaatca ccgcacggga acgcagcctt gccgctttcc atcctggtga aaaaacgcca
    32101 atcacatcat caagaattgc catcagaacc tcgccagccg gtacccggga tgcccccgtc
    32161 gtcgtgtaat cagagccgca aggcggcgct cccactcctg ccgtccctgc cggatctcag
    32221 ataagttttc catggtcatc tgctgaccat taaaagtgac ggattttccg tccagcaccg
    32281 ccatttcagc ttccgtataa cgctgaatca tggcttcaat atcattctga ttcataacca
    32341 tcctccggaa gtcagccagg ggttaacatc gtcagttact gttttcttcc gtttttgttt
    32401 tttaacaggc gtggataccg gctccggtga gggtgacggt tcggtactgt ccgggacaca
    32461 ctccagccag gtttcccgac tcgcccactc cggtgcatcc ggccagcgga tcttttcgta
    32521 tccatgcaga atgaccagcg cctcggcata caccatcagg tcaaaagctt cgttggcacc
    32581 gcgacccggc ttactccatt tcccgtcact gctccgctct tcatacgtca gttcgtcgta
    32641 aaaccagctc cccagccagt cagggaaatg cacatagcct ggacctggcg agtcacgcca
    32701 taacgcgtta ttcacccggt ctttcagggc atccgtctga agaagccaga gcggcacatc
    32761 acctgcggcc tgcgcccggc ggcccgttcg tccggtgtta tcagggaatg tacggctgat
    32821 cagttttgcg cgccggatgc tgtcgccctt aaacaggtaa atacgtttac caaggccatc
    32881 acgacggcaa cgacgccaga atttataggc attatcagtg accccgtctt caccgccgga
    32941 atccaccgcc attgccatca gtcgcatttg ttgagaagga tcggaggcca gcggccagct
    33001 tttatgaaaa acatccgtca gcaggacatc ccagtcttcc ggatagctgg ccggatcaat
    33061 tcgctggctc tccccgtcgc agtcactgcg caatgactgc gtgatgttgt aacgatcaat
    33121 aatccagcgt tcgccacggc tgccatagcc cgttacctga accacaaaac ggcgatgacg
    33181 tcccgcctgc acatccactg tcgccacaag gaaattaacg ccatccggaa cactgcggga
    33241 aggaactggc tctgcccgct gctcaagcag ttcacttttt cgttgctcca tgctggcacg
    33301 aggaagataa ggtaatcccc agtcggtgtt gataaccgcc ctgagtgttt cttcgcttcc
    33361 tgtcgcttca tactcctgtt ctgcagtcag taatttgtaa accagttgcg cccaggtctg
    33421 atacgcagca gctggccctt ccatccagaa actggcgata cgggagcggc gcggttcacc
    33481 ggaaacgttg ccgttacgat caatgacctg accttcacgt aaccagactc ctgcactatt
    33541 gagctcacgc tttttctccg cagtgataat gccgctgcag tgcgggcaaa gtagatacgc
    33601 cgcctcactg gctttaaagg gatccggttc attacggtag ccggtcatgg catccatagc
    33661 tggctgaaaa tattcaccgc agtgcggaca tggccagtac cagcggcggc ggtcaccacg
    33721 attgtaaagg gaaagaatac cagtcgtcgg tggcgcttca tgaggcgact tacgtcgcca
    33781 tttgctgtcg cagatgtcac gtcccggcga gctctccacc agagtcatcc cggcggacat
    33841 aaatgtggtg gtacgttttg aggccaggga gaaaccatca ccctcgctgt cgatattctc
    33901 cggaaaacgg tcgtaatcgg ttaaggcgac aaaccggtaa tccgacgacg acataatgtt
    33961 gaccgagggc caaccaattt taaggaacga gccatccctg aacgtcttat catggacatt
    34021 attgtcgtta cgacgtggac tcattctttt ctttaccgcc gcactgcttc tgaacgttct
    34081 gtcgaggcgc tttttagaat gctcgcgggc cttatcttcg gtcatctgca caacgagcat
    34141 gtccgaagga tcgcaaacga tggtatagac aatccatcca tcgatcagac caatggtctt
    34201 ccctgttcgc gcaggaccaa caaaaatcac cgcatcgtat tcacgcgatg ccaggcagtt
    34261 catgggctca atgatgtagg gtgtcagttc aggatcccat ggcaccgagt taccagcccc
    34321 cttgggaaca cgcatgaatt ttttaacagc ctccgaaatc ggcatgcgac gtggtgggga
    34381 aaatcctgcc gatatgtccc ttcccaaatt tcgggctgat gaaaaaccca ttattcctcc
    34441 tagagactct ctccttcctc atcaggaatt aattcagcag cacaagcctc gtaggatttt
    34501 tcctgaagag tgtatcgcag gtcatcaatg gcctgctgta caacgccgac ggcctgagga
    34561 gtcagggcgc aatcgcgttc aagaacatcc ggaattgtct ccagaacctg gacgacagcc
    34621 tttctcatgg acgaatagac gatgactact tcatcaactg ggatgagttt tcgctgctcc
    34681 ttttccagct tgatcctttc attttcagac tggtaccagt cttttctctc tttcggctcc
    34741 atacgggatg gatcatgaac agagtctgct gcctcatgct tcacactaaa cagggcgggc
    34801 ccgacatgct gcagggcgta aacggggttc cccctgacag tcgcagccac aggagtgttg
    34861 gccgcgagga gccgtttttt tactgtgtcc cggtgaagcc caaaggcctc ggcgatttta
    34921 aaaacactcc agtaataagc atcaccgatc ccgctcacat ttgacataag caactccatc
    34981 tggcaggtga aaatcaggtt tatttatata tttcaattaa ttgcaaactg gtctaatgac
    35041 agggagaaaa aatattgtac aggtgaaaag agaaataact tttaattatc aataaattac
    35101 caaacatgct gccgccgcca tggaaatgca aaaactagtc tttttccgcg acgctcccgc
    35161 cccgtggcag ggacccccac tgggaggacc cgacagcctg acaaccgtga cgagcatctg
    35221 aaacagagct ttacataatg gcatggggat aattcagaag gacatcacag catgcccaca
    35281 caaattagtg tgagtgtcct gtttcttact acgcgcagga ctgacgagca tgaggggaaa
    35341 atacgcgaac cgaaatgcct tatcttcaga aatgcagccg gcatccgaac tggacgatat
    35401 tgtaaattca cctaaatacg aggcattgct gtagttgaat tgcagtactg tattagcctt
    35461 acagtgacag aatacgatat tgactctgtc acttgtgaaa tggtcagaat ggttagcagt
    35521 tatggtgatc agtcaaccac cagggaataa tccttcgtat tcttatcgtg ctttactaac
    35581 gcggcctcaa ttgccctgaa tgcttccaga gatacttgat gttctataca tgcaattaca
    35641 acatccgggt aacccatcga aatggtgcta ttaagcatag tttttacacg aacctgctcc
    35701 tgggaggggg catcagtgta ttgatcttta tgcattttgt cgctccattc gtttgctctt
    35761 cacttgcggc taaaaatata acgtcaaatc actcagcatg aaagagtgaa agtttcagca
    35821 cgtttccctt acataatctg tcgtttgtat tctcttcagg cataccggaa agactaggta
    35881 ttgtcacaca accaatacca tcgacaatcg agatttatat ctattcggta atgtttaaat
    35941 ataacaaaac cccgtaaaaa cgaggtttat gggtaatttg tattgttgaa tagcatctga
    36001 tgagaaattg atgctaatac tataaacatg ctagattaaa tcaatcttga tatcatagct
    36061 ttcaagacca gtcatttttt cccgtgcagt aaactggata ctggtaactt ctttcccggt
    36121 ctttttctta agctcaatga tttttttgtt atatattcag aaatatctgc ttctgttttt
    36181 gtttttaact cttcaatgtt catcatttcc tcttttagtc tgttatgact gtcctggtac
    36241 acagtagtgt caattatatg gagcaaacgt ataaaagata agatgaaaca tcgcaataat
    36301 caacatacga cggtctaaat ttcacacaaa cagataaaga tgattatcat ttattatcaa
    36361 tgcattagaa tcaaatcaat tcaagagtct cattgctgct tccagaattt cttctgaagt
    36421 tacatgtcga tccgctgcta cataaatgac tttatgatct ccggtcagag atggaaaccc
    36481 tgcagccatt acagtgaggt gtgttgtttc gccatttgga tattcacgca tgatggtgtt
    36541 aactccggtc atcactggca ctaccattgc tggttcagag ttaaaaaaaa ctattatttt
    36601 tttcataatg ttaccgtaat atgtgagtat ccatcgacta gacactaagc aaaaaagctc
    36661 ccgaaggagc cttcattttc actttcttaa atctaacgac agatggctaa catttaagta
    36721 ttgtgaaata ttatcaaatg taatcatcat tgatttacaa aagatacatt ttgccccgaa
    36781 aggatttctg tcagaaacat caaaagatga tgttctatac tgagaaccat ggcaacacgg
    36841 gcatctaaag tgaatatggt ttgtaatatt ttctacctca aagtgccact acatgaacag
    36901 ctgcagggcc tttaggtccg ttctcaatac caaattcgac ttcctgattc tcagttaacg
    36961 ttttgaaatc gttgctctga atagctgaga aatgcacaaa cacatctttg ctaccatctt
    37021 tcggcgtgat gaaaccaaaa cctttttcag ggttaaacca tttcactaaa ccagtcattt
    37081 tgttagacat cattattacc ttttgaagaa gttagccctt gggcagaatg gtccgaaaaa
    37141 aaatatcaga gagaaaaacc aacaaggaaa tctcaagagg tacaaataat aaaattataa
    37201 caatgactgc ttcagataat tttgtaacaa accagaacac cattaacgca tgattaacca
    37261 accatagcaa ggattacttt tgtaaagaaa aacacacgaa tgaaacaata gctttattta
    37321 ttaataaaac gtgtcattct gtctaatgac cttttatctt acccttaaga tttcaaggat
    37381 tttgactcat ggaagagtcc tttttattta aatttcacat tcagcgctaa aaataatccg
    37441 atttaatatt aatctacatc tgatattttt tatctcttaa agattcataa atccgttgac
    37501 aagtcactcc tgcgatgtag cgtttgtcag caatttcagc ataaagctga gcttctgctg
    37561 caatatctcc gagcatgttg gtgagcattc cttcggcggc tttggttgtt ttgcctctga
    37621 cggcagcggc aagatttgcg gtatgctgcg ctgcgtcaag gcgtatggca tattttgttg
    37681 cttcggcacg cagctggtta acactatcag acagatcagc agccctggca gaaatttcag
    37741 cggctttctg ttgtgcatct ttaacagcct catcacggga tatacttcgc ccctgttcaa
    37801 ttattcgaac agcaaattgg acatttacct cttgagataa ttcggcagca tcacgctccg
    37861 cccatttttt ttgccatcct ctgtcgctcc agacattacc tacgacaaat cctaccaaca
    37921 cgagcaaaat caccgtgaat atctgattca ctgttctatc ccccagcagg ttaatgcact
    37981 ctcctggtca cgacgaataa cctgaccgta acaattattt gaacgaatgc ggcaatcacg
    38041 tccaccgtcc tttatccacc agcgaatcgc ctcgcatgcg cctttacgat caccggcatt
    38101 aagccgctta taaaacgtcg acggaaaaca cttaccaggg ccaatgttat agggacagaa
    38161 tgacgcgata ccggctttct gtggctcggt cagtggaact tttatattgc gctccaccca
    38221 tgccagcgcc ttatcacgct caatggcgtt gacctggtcg catttttcct tcgacagttt
    38281 catattggga aaaacggttt ttccatccac cactgtggca ccccgacaga tggtccatat
    38341 gccagaacca tcgcggtatg ccattgtgtg gttaccttct ttttcgtcca gaaactggtc
    38401 aagtatctga ggagcagatg cgccagcacc aatcagcgcc agaacggcag ccgacaggcc
    38461 gtatctgatt tttgtgttca tagatattta tgatgaggac gctcgtgctt attggcagga
    38521 ttttcaatct taaaggagta ctgatgctgc agataagact caactttttc tgacaatttt
    38581 tctgctactt ccaggaagac ttgccggacg ctcattctgg ctgctgcctc ataaaactcc
    38641 agcgcagctc cttcaacacg gtccatggcg acatccaggc caaaaatttc accgtcaaag
    38701 cgttctttgt cctgtaaggc tacagttacc gtaactttat tctcaaaatt acggactcct
    38761 ttcacaacca gttcatagtc ttgagtcatt ggattactct cctctcgcag ccttacgcct
    38821 gtcttcttta atcttgaaat aaagatttgt caggtacgtc agcaggccaa ataccaggct
    38881 acccagcaca cctattgctg cccactgtga gggcgtgact ttatcgagca gctgtaaaaa
    38941 ccagtagcca gcactgcctg cggaggtgcc gtaggcaatg cctgttgaaa ttttgtccat
    39001 ggatttcata gcctcacctc cgcacggaac ggatggcata gttattatgt gtaggcttcc
    39061 agacacatca atcagagcct taattgatat atatgctgga gacgatgcaa tataaaaagc
    39121 tcgccgtagc gagctaataa aatgtatttc tctgatatta tgtttatttg tattagctca
    39181 gacttgacat cacaggtttc gtatatagaa catcatcaaa tctgtcagtt tgctatgaat
    39241 gagatatagt aattgaagag ctaacctcgc atgtcaaagc cagatttctg aaaatctctg
    39301 tagacttccg gattgttgaa ggccggaaat ttggctttat gagctgcgga ctttatcgct
    39361 tcgcaatagg ctttatcacc gttactggta gatattttta acgccgtgcc atcctgagag
    39421 aattccatat gcaacctgca ttttttccct ttccagttat gcggctcatc aagtttggca
    39481 ttaattgcag ctctgattcc ccgcgcttgc gccccccatt catcctgatc atcccagcgt
    39541 cctgaactgc aactacctgt agcagtagtt ttgtggcaat ctgaagggtg taaaggtgtg
    39601 catcccgcaa caaaaccgac ccaaaaagtc aacataacga ttttctttaa tcccacttct
    39661 tgctcctcaa tccattaaaa tctcagcaat agtagttgtt acgtccgcca ctggctcaga
    39721 gctgactatc cgctagattt agctcagtgc cgtagttgtg tcagaacaaa cctaagccga
    39781 aaccgtttat tacaaaacaa taaatatcag ggtttaaaat ccagcacccc attttgaaat
    39841 actttatata cttccggcga agggggggca ggtatatcag cattctttat cgcattcatc
    39901 gcttcacgac ataaatcgag gtctccactt tctcttttaa cctccagtag aaggccattc
    39961 ggggccatat gcattctcag tgtacactct tttcctgaat acttactcgc atccccgaac
    40021 tgtttttcga tggcgctctt gatttgatgg gcatacagac ggatatcctc actaacatca
    40081 gaagtacgtt cagatgaact cacatactgt gtctctattg ctttatcgga gtaatatgat
    40141 gtacggtcat gataatttgt cgatacagca tcagtgcacc cgataataat cccactaata
    40201 atcaacgtaa gaattgatgc gctacgaaaa cccatttttc ctcacatatg tcatatagta
    40261 aaggattata tataccgttg ttttggacgc tcaaacagcg aatcagatca aataaaacgc
    40321 acatttgtta acatttacac aaagtctgcg tgggatattc tgaaagaata tccataatgt
    40381 ggagagaatc tattgaagtg catggtgccg ggtgcctccc ggtgaattca gtaccagcac
    40441 ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac agggggattc
    40501 accatgccag tttcttttaa caaactcccc gcaaaccaga gaacagtcaa ccgcctgaat
    40561 tgtgaagtat ttaaaaattt ctcccgctaa ctgatacccg gctaacagtc tggcgttttc
    40621 tttttcagca acgggaaagc aacaactacc acacccgcca ccagcacacc gtcagccagc
    40681 actgacatta tccggctgct gcaatgccat tcacaaaaac agtaagcaat cactttttac
    40741 cgtaacaggt gataatccag atatgtatct accccagatg agtaatccga agttcatcca
    40801 taccacaggt cctggctatt ctgttgtact cctgaacaag agcaaataat tctgaattag
    40861 caaccatgaa ctcatcgcaa accctctgta tagcatcact attcagaata ataacgtctc
    40921 ttcccgaaag acgatcagga gcacagaaca aaactgtcaa acggctgaag gcctttgctc
    40981 gtgctgcatt gactatatca atacgctgcc taaggatgaa acaccccgac gcctcatcaa
    41041 tattcactct acccacacca tatgaatgat aaatatttaa tgctgaaaaa accattagac
    41101 cgtataacaa acactcaatc aatacttaac agaactttta tttttgacaa acatattatg
    41161 ttttcaacaa tatcctgagc caggtatatt ccagtataag gctctgccgg aaggaatctg
    41221 gaagaatgaa tatggcgcgc tgtactggat tcgaaccagt gaccgattgc ttagaaggca
    41281 attgctctgt ccggctgagc taacaacgct gaataccgat aatggaccgc catcggggac
    41341 ccgcccccgc accaacaacc ctgttatcgt gtcgtctgct cttcctgata agctaatggc
    41401 ggtttgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta tgagtcgctc
    41461 gctctcacca ctgagctaaa gggccgggag cagaataata acggtccgta attaattccg
    41521 caataaaaaa cccgctcggc ggcgggttgt agaaactctt ctaacgtcag gcataaaaag
    41581 cccatcatta tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac
    41641 cttttttaac tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc
    41701 atagaatttc ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca
    41761 tatagcatta aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg
    41821 gcagtctctg ataacaggca taccacgttt taccgactct tcacggtgaa tggcgacacc
    41881 acgcccacgg caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca
    41941 ggcaactttt accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc
    42001 tttcgtacac tttgcccata ccggcggctt accatccgga tactggacct tgtttgtaaa
    42061 aactacgctt tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc
    42121 gctgcgggca taatcctcaa aagcgtacga agccataatg cgcatcacta acggttttat
    42181 ttctgccgga agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc
    42241 cagtaattct gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc
    42301 agaaaacccc atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat
    42361 actcagcgca tcttttgacg ttgaggccga tgcatcggtc aggcaagggg attttgggga
    42421 gtagtatttc ggtaaatctt ccagtttcat tttttgacct gctcttcatt cattatgggg
    42481 taaatcttca cccccatacg tccaccagat actggctgac cacgaacgat attgatttca
    42541 tcaaactgct catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc
    42601 aggatattgt ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc
    42661 agccttccgg acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg
    42721 cgataacgct ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg
    42781 ttcaccgtcg gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca
    42841 agcacgccag ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca
    42901 tgcttttctt cgaacgccag cggatccgca tgaagctcgt tgtgatgctc ccgacacagc
    42961 ggtagcgtga aaatatcgtg agattttgtc cccattccgc cctgaccatg accaatcagg
    43021 tgatggggat cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt
    43081 gtgtatttct cgttaaccca gcggcgacgt ttaggtcgtt tcataaaaga ttccggagac
    43141 tcaggatcaa cggcaatgct gaccaccgtc ttttcctgtg gtgggttctg ttgctggtgg
    43201 gcgtgaggca gcggcgcaag attttttgtg cgctgtttca gtatgctggt ggcggtctgc
    43261 tctcccggta cgatgtcgct ttcacggtac attgagcgga ttttttccgc acgcaacccc
    43321 agcgaacgac gtaataccgc ttccggtagc gcgtccgcca gctgattgcg gaccgcccac
    43381 caggataatt cagccagaga taattcacgc tcctgcgtac cgcttattgc gtgaccgatg
    43441 acgtcaatca tccatgctga caggttttga tgagcaagtt gctcgagtga ttcggatgtc
    43501 tggtcacgca gctggttgtc gcagtgccag cacaacacca ttgcgccggt accatatcgg
    43561 tgaataacgg tttcgctgtg atgataatcg ccgtgtggcc actggcagga tttaatatgg
    43621 cgcaacagcc agtcagacaa tgcaccagca ccaccagcag cacgaattac ccgtgcgtta
    43681 ctgaaaaacg gcagcaatgt tttgtcttcc accagcggct ggcgaacggc aggaacgacc
    43741 ccggacggca gattacgcat gcttttcggt tccggctcca ccagtacccg ggtattgtgg
    43801 aataccggca tggattcacg gcccggctta acgaccacca gcccgagttc cggtaccaga
    43861 acaggtcgaa gtaatacccg cacgttacct ccagatgcgt tgctggaatg tacgggacgg
    43921 acgcggtggg cgttcggagt aaggcaatct gactgagatt atccagtgac ggtagtcgag
    43981 actaagggct ttcttaacct cgtatccgcg cctgcggtaa cactgaatta tccattcagc
    44041 ctgctcttca gtgcatggag ggtgcaggaa ccattcagac ttgaatgcgt gagaataccg
    44101 ctcgtgcgtg caggcaagaa cgggcgaatt atcagaattg taatatttta cgttgcgtgc
    44161 catcggtttt ctccggtggc acggtgttac tcagcgggag ttcagccccg cgcaagattg
    44221 tagatgagtt tattcttctg caaaagctga aaagcctgct tttattccga tctctttcag
    44281 tgcctgtaat gaagtgacaa actcaccttc gcgcaagata aatccgtctg tcactcgacc
    44341 atccacaaaa ttaattaacg cagccccatt ctttcgcaaa cacataatgc ggtaatgact
    44401 aacaagattt ccattttcaa cgcacacagc atagaggcca tcttcacaaa aaattttacg
    44461 cagttcttcg atgttcatca tcagaatcct tccggataat tagctctccc ctttaaggga
    44521 ccatccctct tatccctgcg cgctacttaa gtatttttga ttctattccg gcaccgtcca
    44581 gaacttcaaa cgcgttgaaa ataaaaacaa aaacccgccg aagcgggtta agtgcgggtg
    44641 cgttgaggat gcctgccaca tcagaggtgg cgagggattt ctccctcgcc gggtctctta
    44701 ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc tgcccggttc ggattcgtac
    44761 ctcgcagagg tctttcctcg ttaccagtac cgtcactatg acggttaaac agatgacgat
    44821 cagggcgatt aacatcgcct tttgctgctt catagcctgc ttctccttgc ctttcggcac
    44881 gtaagaggct aacctacatg tgtctagcat gaaattggcc tcagattaat gttaagcgtc
    44941 ttgcaggacg cgtaatgtta actggggctt ttctctatct gccgttggtg ttcatgcccg
    45001 aggcagatag cctcaagcac ccgcagccat tctacttaac taccgttacc tcgccaatgt
    45061 gaaatcagtc agaaaggcga tccataagaa caatagcaag acaataaatc gccattacag
    45121 ccgtaatagc cagcgcacat ttgagaacca gcaccacaac ctcctgtatt ggacgtacac
    45181 cagtcctgat gaatatgagg ctgtctcgtc agtgattcaa tacaactatt gggtatagtt
    45241 tctctgattt tttctgtgga aatggggctc aaccactagt caccacctgc acttctttta
    45301 atacgcaaag tccgacacaa gctaaccttt tagtccgctt tgagcgaaca acggaagttc
    45361 ggaataaata ctgacataga cgtgttttct tgcggtggcc agtttttgtt gaccacttca
    45421 ttgtacctaa tcttggaggt tgatcagtta accctcatta ttgcgggtta actgaagtta
    45481 ggcttactgt gtatagataa aacaagcgtt tttccaagtt accttagaaa aagccttaag
    45541 ttagaagccg ttcctcgtaa tcgtcggttg tgggcatttt tccgatctct gtcatgcgac
    45601 acactaatcg ggataaaagc actcgtacct caattatatg ctcaactaaa aagttatctg
    45661 acgtagctcc cccatgcact gccttcgaac ggtagtcata cagcttctta accctacgat
    45721 aggtggctaa acgatcaggc cctcgctcct caagatatgc tgcggccata agagcaagac
    45781 gaaaacgaag ctctgaattg attccaaaga gcgcttcaaa tcctgcccat aaagcagcag
    45841 cagccattct caaattcgcg tgttggttgt gagtagttaa agaatcaata gcgagccgga
    45901 atgctggttg ctctagcaga tctatccaac gcttgagatt ctcttgaacc cagtcgagag
    45961 actctactga tatctctaca cttgggctta ttctccgaac tgccggcacg tcctcaagaa
    46021 gttggacttc acacgagttg gcttgaagag cagggaatac atcccaagat gcacttgcta
    46081 ctgcaggaac aagtatatcg gtgagtgtgc ggcatcggat cgcggatatg atccaccatc
    46141 cgatagtcag gtctgattga gaaaatttcg actcactgct ttgcaacgca agctcatgcg
    46201 taactgcggg catatgacga gcaacagcgc tgagtagcga cttgctctta agcgctttgg
    46261 ctaactctat ctctcctggt ggatgaagta cctcccttaa ttcacctacg tcttggaaaa
    46321 cataaggccc gtttcgaaac gtcaatcctg acaaagcgaa acagatctca ttctgactgg
    46381 ataaactaga tattcgagtt gcagcgtatt tcttgcgaga ctcgaagtag ttaagtaaac
    46441 ctgacaattt ttctttgttg atttccatat tcccaagctt agaattttcg ctattgtgta
    46501 ccgtatatta cacaatagct tagttagggc gtaggcgtcc gcttttggca caaagcggac
    46561 aaccacgcca gatctaccct gtgccatgaa aaatgagaat gtcaattcac tcctgaacta
    46621 atgcttttta atctagtaac gtctaaaata cctaacatta tccctgataa aatgccagta
    46681 tgcgctgcat aacttcactc atccggcact cgcgacagat tatgtttagg cgactgtcgt
    46741 agcgacgtat ttctccatca ggtaacgacc agataaggtc cggatcaatc acagctgttt
    46801 tcttcacctt tgccctcgag agttttttgc gggcgttttg ccagtcctta cgcgcctgtt
    46861 cagacgggaa taacccgtaa ccagagctgt atacatcgcc actggcaacc agctctctgg
    46921 ccagaacgct catcagatat cttgttgccc cagttttagc ttccagttgt cgtaacgtct
    46981 cgcgcccact ctggcgtacg agttccacca cctgcccttt aattttttcc cgctcttctt
    47041 gtgtaaaaac ttttgccaca agtcctcctg aacattactt tatgccctaa aatcaacact
    47101 tatcccctga aaccaggcgg aatttctgta tccggttcag aaatatgatt cacacaacgc
    47161 tgtacagacg aacgccccag acggatgacc agttcatccc atttttcacg aagttttgac
    47221 gggctcatga tgttttttac ccagaatgga tctcgctgta cccgaccaaa catttcgcaa
    47281 atttgtctgt ggcttctgcc atccagcatc cgcattgtgc gcacgtcatt ggcccaggca
    47341 gtccagttag gctcttttgg tcgcatgatc tcgccatcat cactggctgc ctgttcgtag
    47401 agacccacga tccgccccca gatccactgc gcacacgcca aatcctccct gctaccccac
    47461 tggcgttttt tcgcactaaa cacaaccgcg tcggagttcc gggttaaaaa atcctgttca
    47521 gtcgtctgcg ggtccggttg cgaagcttcc ggacgaaaag tgtttttatt ctctgtagta
    47581 atctctgttg tattctctgt aagatcatca ggccattttg acccgatgac attgggtcgt
    47641 tttgaaccaa tggagcgttt cattttgacc tcttccatcg tgtcattttg acctgatgga
    47701 gcggcgcatt ttgaaccgat ggattcgctc aatttgccac catctaaaag ctcgctccca
    47761 tagttgatcg tgtagaaatt ggtcatatcg cgctttgatt tattgagctt ttcacaacgc
    47821 aaaagcccca gcgttttcag acttgcaaac gcgcgcttta acgttgactc tgaccagaat
    47881 gggaactgtt ccagccattg ttccgttgtg ttgtaaatcc agcgaacacc atcacattcc
    47941 atgccggaat tggtatctct caaccagtaa tgcaactgct gcaacacaat ggcttcattt
    48001 aagccaatct tcatcgcaag ctgtgtgttt ataaccagtg ggcgttcagc aaaaagaagg
    48061 ctcataattc catccagctt tttgttggta ttgctgtcga tacgcaagtt tgaaagcaat
    48121 tgctttttct ataagttcgt cagtttcacg atccactacg gcaggatcag caaaaagcag
    48181 tccggactcc accacatcgc catattcttt gtttaacccg gcgatcatgt acgtgatgct
    48241 ttttccgtca ctaatttcac gatacaacct gaaatcatta atccggatag cctccataat
    48301 tgcaggcact agcgccgtga acttttcacg cttatccctg gtgtcgatag ccttccagcg
    48361 ttcgaatatc ttcactcgat taacgccaag cgctcgctga tcaaccgcgc caccttcatc
    48421 tgtgacacgc tgaacatcga tgttcgggcg ctctttcaaa gcccagaatg cttcagtgat
    48481 taatatcgtc gcctgctcct gtgtcattcc tggtcgacat atccaggcat ccagagcctc
    48541 acgagcctgt tcaggagtga ttttcattgt tcaaccgccc cgcccgcttc gtcttacgat
    48601 attcgtcata aaccttggga tcatactgaa gctcgccgcc agatgcctcc tgtagacgca
    48661 tcgcgcgacc ttcaggaacc agctcttgcc attgagaaac agcagatggg tcaacgccag
    48721 cagctttcgc tactttggct ttcgtaccgt aaaaattaat tacgtctgat ttaaacatcg
    48781 cacctccaat attgagtttt ctcaatgcta atcactcaag gaatctcaag tcaagggtta
    48841 ttaagatatc taaatatgaa cgagaaaact ttaggtcaac gcattagaga aagacgtaag
    48901 caggtaggct taagtcaaaa cggtttaagc aaagctgctg gcgtatctgg ctcatcaatt
    48961 tcattatggg aaagtgacca tacagccccg cgcgggcaaa atttgcatcg cctcgccgag
    49021 gtattgcaat gttcaccaac ctggatactg tttggcgacg aggacaaaac accagctccc
    49081 cctgtttcac tcgatagtgc cttagactta tcggaagatg agttagagat gctgcgtttg
    49141 tatcgcgcac ttcctaaatc agagcaacaa gcacaactca gcgaactccg cgcccgcgtt
    49201 gagaatttta atcgcctgtt caccgagcta ttagaggctc gcaaacgcaa caagcatcaa
    49261 taatcatccc ttcacaaaat tttaaagcct tacatttcaa tgtattggct ttatcttgcg
    49321 ccaatacttg agttttctca tcaaaaaacc ttgacgaaaa ataatgagaa aactaaatta
    49381 cctccatcaa gacaccgcac ggtgttctca gcaaacagtt ccgctacccc ggcgttaagg
    49441 ggaaatgagg tcagcatgga tactatcgat cttggcaaca acgaatctct ggtgtacggc
    49501 gtgtttccaa accaggacgg cacgttcacc gcaatgacgt ataccaaaag caaaacgttt
    49561 aaaaccgaaa atggtgcccg tcgctggctg gaaagaaact caggtgagtg atatggattt
    49621 cgacacaatc atggaaaagg cttacgaaga atacttcgaa ggccttgccg aaggcgaaga
    49681 agctctcagc ttcagtgagt ttaaacaggc actcagaata agaatgtgct ctcacaatga
    49741 cgcggagcac aaatatgaga agcaaaatca gaccgcagaa aattttgttc tggaacccgg
    49801 agaaacgctt ttcaaaattc ccgttacgtg ccccatttgc ggttttacat cagaagaact
    49861 tgacgactcc tgtaacaatc aggaaacaac caagtatgtc gaagatgata ccgagtgcgc
    49921 acgaagaacg attatatcca caagtccaaa ctccaggacc aataaatctc actttgagag
    49981 ggtgattaat ccactccccc aaaccaataa aaaagatgcc ggaggacaaa aaacccaatg
    50041 gaacaacggg atatctgtca aaaaaagacg ttccattaaa gacaaacaac gcagcgccaa
    50101 caactgtaag cgctttatac cagtaatcaa ttttcatgtt cttaagcgga tttattggtg
    50161 gttgcgacat tgcttaatga atccttaaaa ctgtggtgat tttaaggata ccacctcgcc
    50221 tgacgtggtt aaaagcaggc acacaacacg aaagcgcacg gcgaagttcg tctcactgta
    50281 cggtgtcgtt aaatttaatt cgaccgtgcg cttccggttg tggcaacccg caaaatggcg
    50341 cggcggtaag tatggcgggg ttattccttc cccgttgagg acaccgagtt gtcaggttga
    50401 ccatacgctt aagtgacaac cccgctgcaa cgccctctgt tatcaatttt ctggtgacgt
    50461 ttggcggtat cagttttact ccgtgactgc tctgccgccg tttttaaagt gaattttgtg
    50521 atgcggtgaa tgcggctaag cgcacgcgga acagttaaaa ccaaaaacag tgttatgggt
    50581 ggattctctg tatccggcgt taattgttaa ctggttaacg tcacctggag gcaccaggca
    50641 ctgcatcaca aaattcattg ttgaggacgc gataatgaaa acgttattac caaacgttaa
    50701 tacgtctgaa ggttgttttg aaattggtgt cactatcagt aaccctgtat ttactgaaga
    50761 tgccattaac aagagaaaac aagaacggga gctattaaat aaaatatgca ttgtttcaat
    50821 gctggctcgt ttacgtctga tgccaaaagg atgtgcacaa tgaattcagc atttgcgctt
    50881 gttctgacag tttttcttgt ttccggagag ccagttgata ttgcagtcag tgttcacagg
    50941 acaatgcagg agtgtatgac tgcagcaacc gaacagaaaa ttcccggtaa ctgttacccg
    51001 gtcgataaag ttattcacca ggataataac gaaatcccgg caggtcttta aaacagttcc
    51061 gtaataaaca tccgatttca ttcttatatg ccagcaatgg cagggatttg ttcaccctta
    51121 aatctgtaat gaggtaaaac aaaatgagta aagtctttat ttgcgccgcc attccggacg
    51181 aacaggcaat aaaggaagaa ggtgccgtcg ctgtagccac tgccattgaa gccggtgatg
    51241 aacgtcgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg gctgctcagg
    51301 actgcgctta taaatttctt gtttgcgagg ataaacccgg tataccccgc cctgccctcg
    51361 attcctggga tgctgaatat atgcaggaaa accgctggga tgaggcgtct gcttcctttg
    51421 tcccggtcga gactgaatcc gatccgatga acgtcacttt tgacaaccta gcccctgaag
    51481 tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcactgtt gatatggtga
    51541 ttagcgcaca ggaattgttg caggaagaca tggcaacatt cgacggccat atcgttgaag
    51601 cgttgatgaa aatgccagaa gttaacgcta tgtatccgga gcttaagctg catgccatcg
    51661 ggtgggttaa gcataaatgt aagcctggtg ccaaatggcc cgaaattcag gcagagatgc
    51721 gcatctggaa aaaacgtcgc gaaggtgaac gcaaggaagc cggaaaatac acgtctgttg
    51781 ttgatctcgc ccgcgccaga gccaatcaac agcacactga aaattcaaca ggaaaaatca
    51841 acccggtcat tgctgccatt catcgcgaat acaagcagac atggaaaaca ctggatgacg
    51901 aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac ggcagcatcc
    51961 atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag cgtatctcgg
    52021 catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt tttggccttg
    52081 tccgtgagcg tccgatcgac attcacaaag atcccgtagc actgaacaaa tatatctgcg
    52141 aatacctgac gacaaagggc gtgtttgaga atgaagaaac agacctgggc actgttgatg
    52201 ttctccagtc atcagaaaca caaactgatg cagtggaaac tgaggtatct gatatcccaa
    52261 aaaatgaaac cgcaccggaa gctgaaccat ctgtagagcg tgaggggccg ttctatttcc
    52321 tcttcgcaga taaggacgga gaaaaatacg gtcgcgcaaa caaactttct ggtctggat
//
LOCUS       DAFATA010000021        46417 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFATA010000021 DAFATA010000000
VERSION     DAFATA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.021"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..758)
                     /gene="msrP"
                     /locus_tag="LGJ20_001650"
     CDS             complement(<1..758)
                     /gene="msrP"
                     /locus_tag="LGJ20_001650"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBK2385723.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPVDKVSGYNNYYEFGLDKADPAA
                     NAGSLKTAPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKS"
     gene            complement(867..1280)
                     /gene="hiuH"
                     /locus_tag="LGJ20_001651"
     CDS             complement(867..1280)
                     /gene="hiuH"
                     /locus_tag="LGJ20_001651"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBK2385724.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            1413..2084
                     /gene="hprR"
                     /locus_tag="LGJ20_001652"
     CDS             1413..2084
                     /gene="hprR"
                     /locus_tag="LGJ20_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBK2385725.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKED
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2084..3442
                     /gene="hprS"
                     /locus_tag="LGJ20_001653"
     CDS             2084..3442
                     /gene="hprS"
                     /locus_tag="LGJ20_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBK2385726.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(3550..4401)
                     /gene="hchA"
                     /locus_tag="LGJ20_001654"
     CDS             complement(3550..4401)
                     /gene="hchA"
                     /locus_tag="LGJ20_001654"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBK2385727.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(4993..6174)
                     /gene="ompC"
                     /locus_tag="LGJ20_001655"
     CDS             complement(4993..6174)
                     /gene="ompC"
                     /locus_tag="LGJ20_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBK2385728.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDKSYARIGFKGETQIADQFTGYGQWEFNIGANGTESDKGNTATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANGVATYRNNGFF
                     GQVDGLNFALQYQSNNENGGPFGQEGSGKGRDIAQENGDGFGMSTSYDFDFGLSLGAA
                     YSNSDRTDNQVHKGTHNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAETRN
                     MTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGNHH
                     YTNKDLVKYVDIGMTYYFKKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLVYQ
                     F"
     gene            6741..7105
                     /locus_tag="LGJ20_001656"
                     /pseudo
     CDS             6741..7105
                     /locus_tag="LGJ20_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            7145..7840
                     /locus_tag="LGJ20_001657"
     CDS             7145..7840
                     /locus_tag="LGJ20_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBK2385729.1"
                     /translation="MDLQHWQAEFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTMEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            7907..9325
                     /gene="dcm"
                     /locus_tag="LGJ20_001658"
     CDS             7907..9325
                     /gene="dcm"
                     /locus_tag="LGJ20_001658"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBK2385730.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            9306..9776
                     /gene="vsr"
                     /locus_tag="LGJ20_001659"
     CDS             9306..9776
                     /gene="vsr"
                     /locus_tag="LGJ20_001659"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBK2385731.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(9765..10685)
                     /gene="yedA"
                     /locus_tag="LGJ20_001660"
     CDS             complement(9765..10685)
                     /gene="yedA"
                     /locus_tag="LGJ20_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBK2385732.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            10858..11775
                     /locus_tag="LGJ20_001661"
     CDS             10858..11775
                     /locus_tag="LGJ20_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBK2385733.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            11854..12036
                     /locus_tag="LGJ20_001662"
     CDS             11854..12036
                     /locus_tag="LGJ20_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBK2385734.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            12274..13968
                     /gene="yedQ"
                     /locus_tag="LGJ20_001663"
     CDS             12274..13968
                     /gene="yedQ"
                     /locus_tag="LGJ20_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBK2385735.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISNSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(13965..14780)
                     /locus_tag="LGJ20_001664"
     CDS             complement(13965..14780)
                     /locus_tag="LGJ20_001664"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBK2385736.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15078..15305)
                     /gene="yodD"
                     /locus_tag="LGJ20_001665"
     CDS             complement(15078..15305)
                     /gene="yodD"
                     /locus_tag="LGJ20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBK2385737.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15468..15656
                     /gene="dsrB"
                     /locus_tag="LGJ20_001666"
     CDS             15468..15656
                     /gene="dsrB"
                     /locus_tag="LGJ20_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBK2385738.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(15700..16323)
                     /gene="rcsA"
                     /locus_tag="LGJ20_001667"
     CDS             complement(15700..16323)
                     /gene="rcsA"
                     /locus_tag="LGJ20_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBK2385739.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16613..17398)
                     /gene="fliR"
                     /locus_tag="LGJ20_001668"
     CDS             complement(16613..17398)
                     /gene="fliR"
                     /locus_tag="LGJ20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBK2385740.1"
                     /translation="MLQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(17406..17675)
                     /gene="fliQ"
                     /locus_tag="LGJ20_001669"
     CDS             complement(17406..17675)
                     /gene="fliQ"
                     /locus_tag="LGJ20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBK2385741.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(17685..18422)
                     /gene="fliP"
                     /locus_tag="LGJ20_001670"
     CDS             complement(17685..18422)
                     /gene="fliP"
                     /locus_tag="LGJ20_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBK2385742.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(18422..18787)
                     /gene="fliO"
                     /locus_tag="LGJ20_001671"
     CDS             complement(18422..18787)
                     /gene="fliO"
                     /locus_tag="LGJ20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBK2385743.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            complement(18790..19203)
                     /gene="fliN"
                     /locus_tag="LGJ20_001672"
     CDS             complement(18790..19203)
                     /gene="fliN"
                     /locus_tag="LGJ20_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBK2385744.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(19200..20204)
                     /gene="fliM"
                     /locus_tag="LGJ20_001673"
     CDS             complement(19200..20204)
                     /gene="fliM"
                     /locus_tag="LGJ20_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBK2385745.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(20209..20673)
                     /gene="fliL"
                     /locus_tag="LGJ20_001674"
     CDS             complement(20209..20673)
                     /gene="fliL"
                     /locus_tag="LGJ20_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBK2385746.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPTPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(20778..21905)
                     /gene="fliK"
                     /locus_tag="LGJ20_001675"
     CDS             complement(20778..21905)
                     /gene="fliK"
                     /locus_tag="LGJ20_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBK2385747.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGVDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(21902..22345)
                     /gene="fliJ"
                     /locus_tag="LGJ20_001676"
     CDS             complement(21902..22345)
                     /gene="fliJ"
                     /locus_tag="LGJ20_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBK2385748.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(22364..23737)
                     /gene="fliI"
                     /locus_tag="LGJ20_001677"
     CDS             complement(22364..23737)
                     /gene="fliI"
                     /locus_tag="LGJ20_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBK2385749.1"
                     /translation="MTTRLTRWLTTLDNFEAKMTQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISTE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(23737..24423)
                     /gene="fliH"
                     /locus_tag="LGJ20_001678"
     CDS             complement(23737..24423)
                     /gene="fliH"
                     /locus_tag="LGJ20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBK2385750.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(24416..25411)
                     /gene="fliG"
                     /locus_tag="LGJ20_001679"
     CDS             complement(24416..25411)
                     /gene="fliG"
                     /locus_tag="LGJ20_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBK2385751.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(25404..27062)
                     /gene="fliF"
                     /locus_tag="LGJ20_001680"
     CDS             complement(25404..27062)
                     /gene="fliF"
                     /locus_tag="LGJ20_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBK2385752.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LIAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            27277..27591
                     /gene="fliE"
                     /locus_tag="LGJ20_001681"
     CDS             27277..27591
                     /gene="fliE"
                     /locus_tag="LGJ20_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBK2385753.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(27985..28653)
                     /locus_tag="LGJ20_001682"
     CDS             complement(27985..28653)
                     /locus_tag="LGJ20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBK2385754.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVRNQGAEL
                     IQPV"
     gene            complement(28762..28995)
                     /gene="yedF"
                     /locus_tag="LGJ20_001683"
     CDS             complement(28762..28995)
                     /gene="yedF"
                     /locus_tag="LGJ20_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBK2385755.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(28992..30197)
                     /gene="yedE"
                     /locus_tag="LGJ20_001684"
     CDS             complement(28992..30197)
                     /gene="yedE"
                     /locus_tag="LGJ20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBK2385756.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMVVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPVGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            30384..30797
                     /locus_tag="LGJ20_001685"
     CDS             30384..30797
                     /locus_tag="LGJ20_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2385757.1"
                     /translation="MKKLAIAGALLLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(30831..32318)
                     /gene="amyA"
                     /locus_tag="LGJ20_001686"
     CDS             complement(30831..32318)
                     /gene="amyA"
                     /locus_tag="LGJ20_001686"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBK2385758.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKLLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(32396..32761)
                     /gene="fliT"
                     /locus_tag="LGJ20_001687"
     CDS             complement(32396..32761)
                     /gene="fliT"
                     /locus_tag="LGJ20_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBK2385759.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDSSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(32761..33171)
                     /gene="fliS"
                     /locus_tag="LGJ20_001688"
     CDS             complement(32761..33171)
                     /gene="fliS"
                     /locus_tag="LGJ20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBK2385760.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(33187..34602)
                     /gene="fliD"
                     /locus_tag="LGJ20_001689"
     CDS             complement(33187..34602)
                     /gene="fliD"
                     /locus_tag="LGJ20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBK2385761.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFAATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKISQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGNDNAMKLSVSGDSALESFMGYNGTSGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDALEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKKDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            34848..36179
                     /locus_tag="LGJ20_001690"
     CDS             34848..36179
                     /locus_tag="LGJ20_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HBK2385762.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGQTISIDL
                     KKIDSSTLGLNGFSVSKNSLNVSNAITSIPQAAGNEPVDVNFGDTDESAAIAAKLGVS
                     DTSSLSLHNILDKDGKATADYVVQSGKDFYAASVNAASGKVTLNTIDVTYDDYANGVD
                     DAKQTGQLIKVSADKDGTAQGFVTLQGKNYSAGDAADILKNGATALELTDLNLSDVTD
                     TNGKVTTTATEQFEGASTEDPLALLDKAIASVDKFRSSLGAVQNRLDSAITNLNNTTT
                     NLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLSKANQVPQQVLSLLQG"
     gene            36499..37218
                     /gene="fliA"
                     /locus_tag="LGJ20_001691"
     CDS             36499..37218
                     /gene="fliA"
                     /locus_tag="LGJ20_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBK2385763.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            37264..37815
                     /gene="fliZ"
                     /locus_tag="LGJ20_001692"
     CDS             37264..37815
                     /gene="fliZ"
                     /locus_tag="LGJ20_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBK2385764.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSNFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLAQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            37903..38703
                     /gene="tcyJ"
                     /locus_tag="LGJ20_001693"
     CDS             37903..38703
                     /gene="tcyJ"
                     /locus_tag="LGJ20_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2385765.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            38808..39794
                     /gene="dcyD"
                     /locus_tag="LGJ20_001694"
     CDS             38808..39794
                     /gene="dcyD"
                     /locus_tag="LGJ20_001694"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBK2385766.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            39809..40477
                     /gene="tcyL"
                     /locus_tag="LGJ20_001695"
     CDS             39809..40477
                     /gene="tcyL"
                     /locus_tag="LGJ20_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBK2385767.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            40474..41226
                     /gene="yecC"
                     /locus_tag="LGJ20_001696"
     CDS             40474..41226
                     /gene="yecC"
                     /locus_tag="LGJ20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBK2385768.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            41456..42178
                     /gene="sdiA"
                     /locus_tag="LGJ20_001697"
     CDS             41456..42178
                     /gene="sdiA"
                     /locus_tag="LGJ20_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBK2385769.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(42246..42470)
                     /locus_tag="LGJ20_001698"
     CDS             complement(42246..42470)
                     /locus_tag="LGJ20_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBK2385770.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(42457..42633)
                     /locus_tag="LGJ20_001699"
     CDS             complement(42457..42633)
                     /locus_tag="LGJ20_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385771.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            42929..43585
                     /gene="uvrY"
                     /locus_tag="LGJ20_001700"
     CDS             42929..43585
                     /gene="uvrY"
                     /locus_tag="LGJ20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBK2385772.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            43582..45414
                     /gene="uvrC"
                     /locus_tag="LGJ20_001701"
     CDS             43582..45414
                     /gene="uvrC"
                     /locus_tag="LGJ20_001701"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK2385773.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYIGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            45471..46019
                     /gene="pgsA"
                     /locus_tag="LGJ20_001702"
     CDS             45471..46019
                     /gene="pgsA"
                     /locus_tag="LGJ20_001702"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK2385774.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            46171..46246
                     /locus_tag="LGJ20_001703"
     tRNA            46171..46246
                     /locus_tag="LGJ20_001703"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46204..46206,aa:Gly,seq:gcc)
     gene            46301..46374
                     /locus_tag="LGJ20_001704"
     tRNA            46301..46374
                     /locus_tag="LGJ20_001704"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46333..46335,aa:Cys,seq:gca)
BASE COUNT        11478 a        11384 c        11899 g        11656 t
ORIGIN      
        1 atcgatttaa tccctttaaa gccatatttc cacggcacaa tcagtcgcac cggcgcgcca
       61 ttttgtggcg gtaacgcctt gccataaaca cccacggtca tcagtgtgag cggatgcatt
      121 gcttcgtcga gacgcaatcc ttcgacataa ggatatttca gcccgccgcc gataaagcgg
      181 tcctgctggc ctggcatctg ttccggtgca taaattgttt cgaaagcgac atacttcgca
      241 ttgctggtgg gttcggcaag cgccagcaat ttgtgcagcg gaaaaccaat ccacggcacc
      301 accatcgacc atgcttctac gcagcgcata cgataaatac gctcttccag cggaaagcga
      361 cgggttaaat catcgtgatc gagggtcaat ggttttgcca cttcgccgct gattttcagt
      421 gtccatggag cggttttcag gctaccagca ttagcggcgg gatcggcttt atccagcccg
      481 aattcatagt agttgttata accggagact ttatctactg gcgtcagtgg cagattattt
      541 tgccaggcgg caggcttgct gaactccagc gcttttccgg cgggggcggg cgggcgatcg
      601 ttccctttaa accagctaag cagatcggca tgcgcagcgt gaggcaaaga aagtgcagct
      661 gcgctgatgc ccagtgcttt taacacctgc cgacgcttca taaagaatac cgactcggcc
      721 gtaacatctg attcttttaa aaattgattc tttttcataa gccatcacac ttttttgtcg
      781 ttagccagca agagccgttt actgttaatc tgtcagggag tgtcctccct aacagtagca
      841 gagatctgct tttacatatt tatgcattaa ctgccacgat aggttgaata cccatattgg
      901 ctaagtaata aaggcacatg ataatgctcg ttcactttat taatatgaaa ttcaacgggg
      961 atctcaggga agaaactttc aagattttgt ttcttgaaat agtccccggt tttaaatacg
     1021 acacggtaat cgcccgtagt tgcagtttgc tcgggccaca gtgccttaat tcgtccatcc
     1081 ttatctgttt tggcggtatt aagttgtaac cagccgttgt ccgctttctt ttcaagagtg
     1141 actgtcacgt cggcagcggg ttttcctgtt tgctggttca aaatgtgcac gctaagaatg
     1201 ttttgttgtg cagcatagac caaagaaggt aatgaaaatg cagccgttac tacggagagt
     1261 actaaataac gctttaacat gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg
     1321 aaaaagcgtc cagggtaacc ttacagcagc attacaattt tgtaatgaac caggctgttt
     1381 ctataacata tgatttatgg catattattt tcatgaagat tttacttatt gaagataatc
     1441 aaaggaccca ggagtgggta acgcaggggc tttccgaagc gggttatgtc attgatgccg
     1501 tttctgatgg cagagatggg ctttatcttg cgctgaagga agattatgca ttgatcattc
     1561 tggatattat gcttccgggt atggatggct ggcagatctt acaaacgtta agaacggcaa
     1621 agcaaacccc tgttatttgc cttactgcaa gggattctgt cgatgacaga gtcagaggac
     1681 tggacagtgg agcaaatgat tatctggtaa aacctttttc attttctgag ttgctggcaa
     1741 gggttcgggc acaattaagg caacatcacg ctttgaattc aacattagaa atcagcggct
     1801 taaaaatgga ctctgttagt caaagtgtga gcagggacaa tatcagtatt acactgacgc
     1861 gcaaggagtt tcagttactt tggctactgg cctccagagc tggcgaaatt atacccagaa
     1921 cggttattgc gagtgaaatt tggggaatca actttgatag tgataccaat acggtggatg
     1981 tcgccattcg caggctccgc gcaaaagttg atgatccttt tcctgaaaag ctaattgcca
     2041 caatccgtgg gatgggatat tcgttcgtag cggtaaaaaa ataatgaaaa gattgtctat
     2101 aaccgtccgt ttaaccttgc tttttatatt gctactgtct gttgctggtg ctggaattgt
     2161 ctggactctc tataatggcc tggcaagtga gttgaaatgg cgcgatgata caacactcat
     2221 taaccggaca gcacagatca agcagttgtt aattgatggg gtaaatccag atacgttacc
     2281 tgtgtacttt aaccggatga tggatgttag tcaggatatc ttgattattc atggtgatag
     2341 catcaataaa attgttaacc ggacaaatgt cagtgatggc atgttaaata acatacctgc
     2401 tagtgagaca atcagcgcag ctggcattta cagaagcatt attaatgata cagagataga
     2461 tgctttacga attaatattg atgaagtttc gccatcatta acggttactg tggctaaatt
     2521 ggcttcagcc agacataaca tgcttgaaca gtataaaatc aatagcatta taatttgcat
     2581 tgtcgccatt atactgtgct cagtattaag tccgctgtta atccgaacgg ggttacgaga
     2641 gatcaaaaag ttgagtggtg taacggaagc gctgaattat aacgatagcc gggagcctgt
     2701 tgaggttagc gcattaccga gagaactaaa acctcttggg caggcgttga ataaaatgca
     2761 tcatgcttta gtcaaagatt ttgagcgtct aagtcaattt gctgacgatc tcgctcatga
     2821 acttagaacg ccaattaatg cattactggg tcagaatcag gttacgctca gtcaaaccag
     2881 aagtatcgct gaatatcaaa aaacaattgc cggtaacatt gaagagctgg aaaatatttc
     2941 gcggttaaca gagaacatac tgtttcttgc cagggcagat aaaaacaatg ttttggtgaa
     3001 actggactcg ctttctctca ataaggaagt cgaaaatttg ttggactatc ttgaatatct
     3061 ttcagacgag aaagagattt gctttaaggt cgagtgcaat cagcaaatct ttgcggataa
     3121 aattttactg caacgaatgt tatcgaatct tattgttaat gccattagat attcaccaga
     3181 aaaatcgcgt attcatataa ccagttttct tgataccaac ggatctctta atattgatat
     3241 cgccagccct ggaacgaaaa ttaatgagcc tgaaaaactc ttccgtagat tttggcgggg
     3301 agataattcg cgtcattccg taggtcaggg actaggcctt tctttagtca aagcgattgc
     3361 cgaattacat gggggaagtg ctacgtatca ctatctcaat aagcataatg tgttccggat
     3421 tacgttgccg caaagaaatt aacatagggc ttcagtacgc cataaattaa ccgtgaaaaa
     3481 acgcagccac gtcgtgagta ctaacgcggc cgcgattgat tatgcgctta cattcaaacg
     3541 taacagggat taacccgcgt aagctgccag catttcctgc gccgccagtt tacccaacgc
     3601 attcgctgca aaaggactgt cgccggtgag aagtttacgg tccttatgta ctcgcccggt
     3661 gatgtcgtca ttaatgatat tcatgcccat tttcttcagt tcttcgccga agtaccaggt
     3721 gagatgaccc ggcatatagc caatctctgg cgtttgtttg tctgcggcgt ctgggaatgc
     3781 gcaaatggaa taaccattca gtgggttatc gccgtggcga agcgccagaa aagccgccgg
     3841 gccgtggcaa agggagataa caaaacggtc atttttgatt gcccactgta aagcggcggc
     3901 tacgtcctgg ctttcaggta aaccaataag tgcgccatga ccaccaggaa caaagattgc
     3961 tgcatattcg ctatcagcgt tgaggctggc gacaacatcc gcgagtttct tcggattgcg
     4021 gaacaacgat ttatgctgct caaagaatgg cattactttt tcatctttgt gcggcatagc
     4081 ccagtattca aacttggtca tcagaccgga aatggtcgcc acttcgaatt cgaaacctgc
     4141 agcatggaga tgatacaacg gcagcaacgt ttcaatcgga tggttaccgg tcgagaacag
     4201 ctttccgtta tcggtcggca aataacgttc gtccgcggcg atcaccagaa ttttatgttt
     4261 accgcgatac ggttttggat agtccacgcc atcaagatca gagacaggac tggtatattg
     4321 gctaagcgaa tattctgaag ggaagaatgc attatcttca gcaatatcga cctgcggatt
     4381 tttacttgtt tgaacagtca tagtgtattc cttattgttg cttagttagg gtagtcacta
     4441 tatttgcgac tgagctggtc gctattttta accaggctag aaaattgagt caatatatcg
     4501 aaagcggtag tcggcgggtg taagtatgta atgatgatgc tgcactggtg ttgaatatta
     4561 ttcacgccaa attttcctta ttgagcaacg tcgtcgcaag tggcctcaat gattttagac
     4621 cattatcaga ggtacggggc gacggggaga gatttagtgc tgtggaatat tgtggtgctg
     4681 atgaatattt atatcccgga ttggtagaga aaaagcttat ttcatgcact atttttgcaa
     4741 atatttctat atgggaggcg gttttgttgc agattaattc ataaagaaat gcatttgggt
     4801 cttggctgga agagtcgctg gtagatgtta ttgtgccagc aggggcaggg taaggtgctg
     4861 tatcgatatg ctttggcaat tgcgcgtgcc gcagagggag ataaaatgcc aataaaaaag
     4921 cctgagttcg atgagggacg aactcaggct agtgttaagt tttacagtaa cacgccgcgg
     4981 cgcgttggct gattagaact ggtagactaa gcccacagcc acgatatcat cggtagcgat
     5041 gccgttttct ttgtagaagt cgtcgtcgtt gtccagcagg ttgattttgt aatcaacata
     5101 agtggacatg tttttcttga agtagtaggt cataccaata tcaacatatt taaccagatc
     5161 tttgttggtg taatgatgat taccctggct gtcgaagtta tcactattta caccgcctaa
     5221 atccttacct ttagattgca ggtaagcgat ggacgggcgc agaccgaagt cgaactgata
     5281 ctgagctaca acttcaaagt tttgggtttt gttagcgatg gcatcagcat caccgtaacc
     5341 agtcatgtta cgggtttcag catacatagc tgctaagtaa acgttattag cgtcgtattt
     5401 agcaccaacg gtccatgctt ctgctgtttc accgccagca gttgcatcga agcgatctcc
     5461 ataacgagtg ttatgagtac ctttgtgaac ctgattgtcg gtacggtcag aattagaata
     5521 tgcagccccc agacttaaac cgaagtcgaa gtcataggaa gtagacatac cgaaaccatc
     5581 accattttct tgagcaatgt cacgaccctt accagaacct tcctgaccga atggaccacc
     5641 gttttcgtta ttactttgat attgcagagc aaagttcagg ccatcgacct gaccaaagaa
     5701 gccattgtta cggtaggttg caacaccatt agcacgaccg ttcatgaagt tgtcagcgcc
     5761 agcataggta tcgccaccaa attctggcag catatcggtc catgcttcta cgtcatatac
     5821 gacaccgtag ttacgaccat agtcgaaagt accattctga ccaaagccta aacctgcaaa
     5881 tgccagacgc gttgcagtat tacctttgtc gctttcagta ccgtttgcgc caatgttaaa
     5941 ttcccactga ccgtaaccag tgaattgatc agcgatctga gtttcacctt tgaaaccgat
     6001 acgggcatat gacttgtcgc catcgctgcc agcatcatca gagaagtagt gcaggcccgc
     6061 tacttttccg tacaaatcca gtttattacc atctttatta tagacttcag ccgcatttgc
     6121 tgcgccagca actaataacg ccgggaccag cattgccaga acttttcttt tcattatgta
     6181 ttcccttgtg attataatct tcatgaatat atcaataagt gccgttatcc aaaaaaaaag
     6241 cacattttga tactattcta tgaagttcat tttattttaa agattacatg taacaaatgt
     6301 atttaaaaga tttcaaaata tttctaatct atttaacata attcaaaatt atgaattcta
     6361 aatataagca ttcatgaaaa ttataaatgt atatcaatat gttatgagtt gaatggaaag
     6421 atagggagta taaatgattg ttgcgccaaa cggcaggcgt gatcacgcgc acattaatga
     6481 aggtataaaa aaaagccggc atcatgccgg cccaaaaata taaacaagac gataaaaggc
     6541 taataacgag gttatcatga cacagaaatc aaaaaaggta taattgtatt cagtgctaat
     6601 atattgtttg ggtgaattaa taaaactatt accaaggata taaataaatc tttattgctg
     6661 tttttccagt aattattgcc gttgcgcaaa atttatctat ttgttcaaaa aatgagtgag
     6721 tcttgactgg ctcatctaat gtggaaaaat gtgactttta tcacataata gtactaagtc
     6781 tgaattttcc gggttatctc aaaatggaat acggttcgac aaagatggaa gagagactct
     6841 ctcgcagccc tggcggcaaa ctggctttgt gggctttcta tacatggtgt ggctattttg
     6901 tctgggcaat ggcgcgttac atctgggtga tgagccggat ccccgatgcc ccgtgtcggg
     6961 atttgaaagc gatttaggct ctaccgccgg aaagtggtta ggggcactgg tcggattttt
     7021 atttatggct ctggtcgggg cattgttagg cagcattgcc tggtacaccc gacctcgtcc
     7081 cgcacgtagc agacgctatg aataaaaact gctaaggtgg cgactctttg gcaacggagc
     7141 gcgtatggac ttacaacact ggcaggcaga gttcgaaaac tggttgaaaa accatcatca
     7201 gcaccaggac gcggcccatg acgtgtgtca ttttcgccgc gtctgggcta cggcacagaa
     7261 gctggcggca gatgatgacg tcgatatgct ggtgatttta acggcctgtt attttcacga
     7321 tattgtgagc ctggcgaaaa atcatccgca acggcagcat tcttcaatcc tggcggcaga
     7381 agagacgcgt cgcctgctcc gtgaagagtt cgtgcaattt ccggcggaga aaatcgaggc
     7441 cgtttgtcat gccattgccg ctcacagttt cagcgcgcaa atcgccccct taacaatgga
     7501 agctaaaatc gtccaggatg cggatcggct ggaagccctg ggggcgattg gcctggcgcg
     7561 cgtgtttgcc gtttcagggg cattgggcgt ggcgctgttt gatggcgaag atccgctcgc
     7621 acagcatcgc ccgcttgatg ataaacgcta cgcgctggat catttccaga ctaagttgct
     7681 gaagctaccg caaaccatgc aaaccgtaag aggcaagcag ctggcgcagc acaacgcgca
     7741 ttttttagtc gagtttatgg cgaagctcag tgccgaactg gcgggggaga atgaaggtgt
     7801 cgatcacaag gtgatagatg cgttttcacc cgcgggataa gcgcgtggcc ctaaatggct
     7861 gtaattatgt taacctgtcg gccatctcag atggccggtt aaatctatgc aggaaaatat
     7921 atcagtaacc gattcataca gcaccgggaa tgccgcacag gcaatgctgg agaaactgct
     7981 gcaaatttat gatgttaaaa cgctggtggc gcagcttaat ggtgtgggtg agaatcactg
     8041 gagcgcggca attttaaaac gtgcgctggc gaatgactcg gcatggcatc gtttaagtga
     8101 gaaagagttc gcccatctgc aaacgttgtt acccaaacca ccggcacatc atccgcatta
     8161 tgcgtttcgc tttatcgatc tatttgccgg aattggcggc atccgtcgcg gttttgaatc
     8221 gattggcgga cagtgcgtgt ttaccagcga atggaacaaa catgcggtac gcacttataa
     8281 agccaatcat tattgcgacc cggcgacgca tcattttaat gaagatatcc gcgacatcac
     8341 cctcagccat aaagaaggcg tgagtgatga ggcggcggcg gaacatattc gtcaacacat
     8401 tcctgaacac gatgttttac tggccggttt cccttgtcag ccattttcgc tggctggcgt
     8461 atcgaaaaag aactcgctcg ggcgggcgca cggttttgcc tgcgataccc agggcacgct
     8521 gttttttgat gtggtgcgca ttatcgacgc gcgtcgtccg gcgatgtttg tgctcgaaaa
     8581 cgtcaaaaac ctgaaaagtc acgaccaggg taaaacgttc cgcatcatca tgcagacgct
     8641 ggacgaactg ggctatgacg tggctgatgc agaagataac gggccggacg atccgaaaat
     8701 catcgacggc aaacattttc tgccgcagca ccgtgaacgc atcgtgctgg tgggttttcg
     8761 tcgcgatctg aatctgaaag ccgattttac cctgcgtgat atcagcgaat gtttccctgc
     8821 gcagcgagtg acgctggcgc agctgctgga cccgatggtc gaggcgaaat atatcctgac
     8881 gccggtgctg tggaagtacc tctatcgata tgcgaaaaaa catcaggcgc gcggtaacgg
     8941 cttcggttat ggaatggttt atccgaacaa tccgcaaagc gtcacgcgta cgctgtctgc
     9001 gcgttattac aaagatggcg cggaaatttt aatcgatcgc ggctgggata tggccaaagg
     9061 tgagaaagac tttgacgatc cgctgaatca gcaacatcgt ccacgtcggt taacgcctcg
     9121 ggaatgcgcg cgcttaatgg gttttgaagc gccgggagaa gcgaaattcc gcattccggt
     9181 ttcggacact caggcctatc gccagttcgg taactcggtg gtcgtgccgg tctttgccgc
     9241 ggtggcaaaa ctgcttgagc caaaaatcaa acaggcggtg gcgttgcgtc agcaagaggc
     9301 acaacatggc cgacgttcac gataaggcca ctcgcagcaa aaatatgcgc gcgattgcca
     9361 cgcgtgatac ggcgattgag aagcgcctcg ccagtctgtt aaccgggcag ggcctggcgt
     9421 ttcgcgttca ggatgccagt ctgcccggac gcccggattt tgtcgttgat gaatatcgtt
     9481 gcgtgatatt tacccacggc tgcttctggc accatcatca ctgctatctg tttaaagtgc
     9541 ctgcgactcg aaccgagttc tggctggaga agataggtaa aaatgttgag cgggatcgcc
     9601 gtgatatcag tcgcttgcag gagcttggct ggcgcgtact gattgtctgg gagtgtgcgt
     9661 tacgtgggcg cgagaagctg acggatgaag cgcttagcga gcgtctggaa gagtggatct
     9721 gcggcgaagg tgccagcgcg cagatcgaca cgcaggggat tcatttactc gcttgatgcg
     9781 tcctgaataa ctggcgcaac tacgggtttt gccgggaaga gatattttcc caacgtgacc
     9841 agtaccaccg cgaagacaat cacgccgagc gccaaccatt caatcttcga cagcgtttct
     9901 ccgcccagtc ccgtacccag caagaccgcg accaccgggt taacgtaggc gtagctggtg
     9961 gcgagagccg gactgacgtt gcggattaaa tacatataag cgttaatggc gataatcgaa
    10021 ccaaacagcg ccagatagcc gaccgcaagg aagcctgaaa gggaagggag cgccgtcagt
    10081 ttttcacctg caatcatcga cgcgatcatt aacaccacgc ctgccgccag catctcaatc
    10141 gcacccgcca tcatccctac aggtaaggta atgcgcgagc cataaaccga gccaaacgcc
    10201 cagctaatcg agccgattaa aatcagaatc gcgccccacg gattgccgct taaatttcca
    10261 ccgctattga gcatgatgat tccggcaagc ccaatggcga tacccaccca ttccagtttg
    10321 cgcgttttaa tgccaaacag gcggctgaag cacagggtaa agaggggcac ggttgcaacc
    10381 acaacggcgg cgatgccgga aggaacattt tgatgctcgg caaccgtcac catgccatta
    10441 ccgacagcca gcaataacag gccaatcagc gcggcattga gcagcggacg tagcgggggg
    10501 agtttgtgtc cgcgcagtag caaaaatgcc agcaataaaa taccggctgc caggaatcga
    10561 acgcccgcca tcattaacgg aggccagctt tccacgccaa tccgaatgac aaaataggtt
    10621 gagccccaaa tgatatacaa cgcaaacagc gcgccaaaaa gcggtaacaa ctggcggaaa
    10681 cgcataatcc ctcacggtgg aaataaaaag gtggttcata gtaaacgtga aaatcattct
    10741 gctggcgaga gatataattg cacttgattg ttaaaaaaat gttgacctgt gaagcaagta
    10801 tcagagctgc cgtttttgct tcatacttac acccttcaac aataaaaatg agagggaatg
    10861 cttttggccg ggagtagttt actgacgttg ctcgatgata tcgccacact actggacgat
    10921 atctccgtga tgggcaaact ggcggcgaag aaaaccgccg gtgtattagg ggatgactta
    10981 tcgctcaatg cgcaacaagt ttcaggcgtg cgggccaacc gggaacttcc cgtggtctgg
    11041 ggcgtggcga aaggatcgct gattaataaa gtgattctgg tgccactggc gctgatcatc
    11101 agtgcgttta tcccgtgggc gattacgcct ctgttgatga ttggtggcgc gtttctctgc
    11161 tttgaagggg tagagaaagt gctgcatatg ctggaggcgc gtaaacataa agaagatccg
    11221 gcgcagagtc agcagcgtct ggagaagctg gcggcgcagg atccgctgaa gtttgaaaag
    11281 gacaaaataa aaggggcgat tcgtaccgat tttatattgt ctgccgaaat cgtcgccatc
    11341 acgctgggga ttgtggccga agcgccgttg cttaatcagg tgctggtgct ttcaggcatc
    11401 gcgctggtag tgaccgtggg cgtctacggt ctggtagggg ttatcgttaa gattgatgac
    11461 ctggggtatt ggctggcgga aaaatccagc gcgctgatgc aggcattagg taaagggtta
    11521 ttgatcattg cgccctggct gatgaaagcg ttatcgattg tcggcacgct ggcgatgttc
    11581 ctcgtcggcg gcgggattgt ggtacatggt attgcgccgc tgcatcacgc cattgaacat
    11641 ttcgccgggc agcaaagtgc agtggtggcg atgatattac cgactgtttt aaatctgatt
    11701 cttggattta tcatcggcgg catcgtggtg ctgggagtga aagccgtagc gaaaatgcgc
    11761 ggtcaggcac attaatactc gcacaggcta ttatgcaaaa ttcgctatct tcaactaagg
    11821 tgaacaagtt tcactcgaaa aaaggaggcg agtatgagct ttatggttag tgaggaagtt
    11881 acggttaaag agggcggccc gcggatgatt gtcactggat actccagcgg tatggttgag
    11941 tgtcgttggt atgacggtta cggggtcaag cgggaagctt ttcatgaaac cgagcttgtt
    12001 ccaggggagg ggagtcgtcc tgcggaagaa gtttgaaagg gtatcattaa cacccgaccg
    12061 gatgtggcgc tcacgtcgca tccggtcggg tgtgccagtg tctgcactga attgcctgat
    12121 gcgacgctat cgcgtcttat catgcctacg ttataaaact atcgatgtct gtcgcgtctt
    12181 atcatgccac cattataaaa ttatcgctgt tttgcggtac gctatcgcca ggttatgcca
    12241 gaatcataaa aaagcaggtt gggagtcgtc agggtgcagc acgagacaaa aatggaaaac
    12301 cagagctggt tgaaaaaact cgcacgccgc ctggggcctg gtcatgtcgt taatctctgc
    12361 tttatcgtgg tattgctttt ttccaccttg ctcacctggc gtgaagtagt ggtgctggaa
    12421 gatgcctata tctccagcca gcgtaatcat ctggaaaacg tagccaacgc gctcgataag
    12481 catttgcagt ataacgtcga caaactgatc tttttgcgta atggcatgcg cgaagctctc
    12541 gtagcgccac tggatttcac ctctctgcgt aatgctgtaa ccgagttcga acagcatcgc
    12601 gacgagcacg cctggcaaat cgaactcaac cgacgacgca ccctgccagt caatggcgtg
    12661 tcggatgcat tagtcagcga ggggaatctc ctgtctcgcg aaaatgaaag cctcgacaat
    12721 gaaattaccg ctgcactgga agttggttac ttgctgcgac tggcgcacaa cacctcgtcg
    12781 atggttgaac aggcgatgta tgtctcgcgt gccggatttt acgtttcgac gcagccgacc
    12841 ttgtttacgc gcaatgtacc aacgcgttat tacggctatg tcacccaacc ctggtttatc
    12901 ggccattcgc aacgagaaaa tcgtcaccgc gcggtacgct ggtttacttc gcaaccggaa
    12961 cacgccagca atactgaacc gcaggttacc gtcagtgttc cggtagacag taataactac
    13021 tggtatggcg tgctggggat gagtattccc gtgcgtacca tgcagcaatt tttaagaaac
    13081 gccatcgata aaaacctcga tggtgagtat cagctctatg acagtaagct gagatttttg
    13141 acctcttcca atcctgatca tccaacaggg aatatttttg atcctcgtga actggccttg
    13201 ctggcgcagg caatggaaca tgacacgcgg ggcggcattc gtatggacag tcgctatgtt
    13261 agttgggaac gtctggacca tttcgacggt gtgctggtgc gcgtccatac gctaagcgaa
    13321 ggcgtgcgcg gcgatttcgg cagtatcagc attgcattaa ccctgctgtg ggcgctcttt
    13381 accaccatgt tactcatctc ctggtatgtg attcgccgga tggtcagcaa catgtatgtt
    13441 ctgcaaagct cgttgcagtg gcaggcgtgg cacgacacct taacccgttt atataatcgt
    13501 ggcgcactgt tcgaaaaagc ccgtccgctc gctaaattgt gtcagacgca ccaacatcct
    13561 ttttctgtca ttcaggtcga tcttgaccat tttaaagcga ttaatgaccg ctttggtcat
    13621 caggcgggcg accgtgttct ttctcatgct gcaggattaa ttagcaattc cttgcgtgcg
    13681 caggacgttg ccgggcgggt cggtggtgag gagttttgtg tgattctgcc aggcgcgagt
    13741 ctgacggagg ctgcggaggt cgcagaacgt attcgcctga agctaaatga aaaagagatg
    13801 ttgatcgcca agagtacgac gatacgtatc agtgcctcgc tgggggtaag tagcagcgag
    13861 gaaaccggtg attatgattt tgaacaactc cagtcactgg ctgaccgtcg gctttatctc
    13921 gctaaacagg ccgggcgtaa tcgggtatgc gcgagcgata acgcttaacg agcggagaaa
    13981 aaatggtcca gcccttcacg ccagccttcc ggtccttcac gctgcgttcg ccagacgcgg
    14041 gtcggatcct catcatgcag atgcacccct tcacggttta gccctttcac aatcaccgcg
    14101 taatccatta cctccagtaa gggcgcatcg tttggcccat cgcccaggcc aagtgtggtt
    14161 gggcgtttgc ctgataattg ttgataggtc gcgataatcc agttggcggc ctgatctttt
    14221 ccggcagagg catccaggac gtgccagaag cgcgcaccct gcataaactg taagcccagt
    14281 tcgttcagac gagcggtaaa ttgtgccata cgctcgtcac tgtcgcgcca gattagcgtt
    14341 accgacgcct catgcagctg cgtcagcgcc gcctggctac ggcttaatcc cgtccattcg
    14401 gcgatggttg catcgtcgac atcatcgaaa gtcgtgaatt taaaatgttc tttctcgcgt
    14461 agcgtattta aaacctggct gatttcgcca tggctaatac ctgagatgat gcgtggaaaa
    14521 ccgtctatat cctgccattg ttcagcaagc tggatcactg cgccattttc ggcaatcagc
    14581 ggtaaccctt gtagccccaa cgttttttgc aagtacagca tttccgctga agttttactg
    14641 ctacagagaa tgacgggaac attcgcttcg cgtaaacggc tgagccaggg ggcagccggt
    14701 tgccagtcat aactatgact gtccagcagg gtgccatcaa gatcgctaaa aaccagtagt
    14761 ggttgttgaa ttgaaaacat ggtcgcgcag ttctcccctt accaggatga ttctcaaaac
    14821 agcacaaata atcaaagatg attttttcgg gagcgatccc gttgacgcca gagccaccaa
    14881 ggggtgacgc acgtcacatt tctattcata agtagcgtta atcattcata tcgcgaatat
    14941 tttcttgtca gcgaaaaaaa ttgcggataa ggtgatgaac acatcagatt tcctggtgta
    15001 acgaattttt taagtgcttc ttgcttaagc aagtttcatc ccgcccccct cagggtcggg
    15061 atttttttat tgtgcattca acgattcact tcagagacgc tgaagtcatg aatatccagt
    15121 tcgaaggcat ccgccaattc acgccatgta tgatattcac gtccatcgac gctaacgtgt
    15181 tcagaatcgt tctggctgcg atgaacttcg ctttcgctaa tttcattgat cgccgccagc
    15241 agggcatcga catcgacgct gacctcacgt tttgcggtat cgctgtactc ttttgcggtt
    15301 ttcatcatct ttcctcgcaa ccgttactct gttgttgccg ccagtcgtta agtccgcctg
    15361 gcatctctta agtatagacc gtcgagaaaa tcagcactcg cgcgcgcctg gcgctgccag
    15421 aaaaacgaaa ttgttctaca ctggcacaaa gccacaggag gaaaacgatg aaggtgaatg
    15481 atcgggtaac agtcaaaacg gatggcggtc cgcgtcgtcc tggcgtggta ctggcagttg
    15541 aggagtttag tgaaggcaca atgtacctgg tttcgctgga agactacccg ctcggcatct
    15601 ggttctttaa tgaagcaggg catcaggacg gtatctttgt ggagaaagca gagtaatctt
    15661 attcagcctg actggtggga aaccaccagt cagaatgtgt tagcgcatgt tgacaaaaat
    15721 accattagtc acattatccg tcagtcggac gacatggtag ataacctgtt tattatgcgt
    15781 tttgatctta cgtttaatat tacctttatg cgatgaaacg gtcttggctt tgatattcat
    15841 ttggtcagag atttgaatgg ttccctgacc tgccatccac attcgcaaca tactcgattc
    15901 ggttcggctc aatgataacg tcggcatatt taaaaaagag gttatcgttg tctctttttt
    15961 cagaatatcg ccaaggatat cgtcgagaga ttccggttta atcgatttag aacttatcaa
    16021 taaatttttt ctgaccaata gatattcatc aaaatgaaca ttggcaattg ccataaaaac
    16081 gataaataac gtattgggat gttgattaat gatgtgcttg atgtgctgac tgttagaagc
    16141 atcatggatg aaacagtcct cattaataaa caccactgaa gggcgctgtg aatcacaagc
    16201 tatggcaagg tcatcaacgg tttcaatgtc gttgatttct ctttttttaa cccctctact
    16261 caacagatac ccggttaaac ctagtcgggt gtaactacat aaatccataa taatcgttga
    16321 catggcatac cctcactcaa tgcgtaacga taattcccct tacctgaata tttcatcatg
    16381 actaaacgga acaacatggg tcacctaatg cgccactctc gcgatttttc aggcggactt
    16441 actatcccgt aaagtgttgt ataatttgcc tggaattgtc ttaaagtaaa gtaaatgttg
    16501 cgatatgtga gtgagcttaa aacaaatatt tcgccgcagg agtatcctgg aagatgttcg
    16561 taggtattat ttttcggata atccttagga taacatgata aacgttacgg aattatatta
    16621 atggcaattc actaataata tcagccagca aattaaacat ctcactgaat aaatgttcgc
    16681 aaaaaggcgc aattaacggc attaatgccg ccattaaaga gataccgaca gtcagagtta
    16741 atggaaatcc aataacaaaa atggataatt gcggggccat acgattcagt aaacctaatg
    16801 ccagattcag ggtcagcagc agagtaatga gcggtaacgc cagcatcagc ccgttaagga
    16861 aaatcaaact ccctgctttg gtgagtgcca gaaacgcatt gctgttcaac ggttcgccac
    16921 caatcggcag ggtgtgaaag gtatcgacca gcagtgaaat caaccataaa tgaccgttaa
    16981 atgtcaggaa cagcagtaac gccagcatat ccatgatacg cgctagaacg ggcatgttga
    17041 gatggctggc cggatcgaca aacgtcgcaa atgacagccc catttgcaga ccgataattt
    17101 cgccagcggt tcgcacagcg gcaaaggcaa attgcatggt gaaaccaagc gcaatgccga
    17161 tcaggatctg ctgcacggcc agccacagag caaagaacga aaaaacagga acatcgttgg
    17221 caggtaatga tggggcaata gcgaacgtga tcatcattgc cagacccagt tttacccgtt
    17281 tcggcacgct gcgttcgctc agaattggcg cggtagaaat gagcgccagc acgcgcagta
    17341 acggccagaa gtacagactt agccaggaaa gccattgatc gcttgtcacc tgcaacatag
    17401 tacggctacc cgatgatata cggcaggtta gtgaacaagg tgcggacgta atccagcaac
    17461 agattgagca tccacggtcc ggcaataata atggcaataa atacggcgat gattttcgga
    17521 ataaacgaca gcgtcatttc gttaatctgg gtggcggcct gcaaaatact gatgataagg
    17581 cccgtgacca acgctaccaa caatagcggg gcagccagtg ccagcgcgac tttcatcgct
    17641 tcagtcccca tcatcatgac cgattcaggt gtcattttgc ctctctagct gtaaaagctc
    17701 tgcgccagcg aaccgaccag caattgccag ccatccacca gtacaaacag catcagttta
    17761 aagggcagag caatggtggc ggggggaacc atcatcatcc ctaatgccat caacacgctg
    17821 gctatcacca ggtcgataat caaaaaaggg atgaaaatcg tgaagcctat ctgaaatgcg
    17881 gttttcaact cgctggtcac gtaggccggg agcaaaatgc gcatcggcac ggcttcaggt
    17941 ccctgcaacg ggccagtatt cgccagtctg gcaaacaacc ctaaatccgc ctcacgggtc
    18001 tgacgcagca taaactcacg cagcggctgc gccccttttt ccagcgcctc ctgcattgat
    18061 attttctctt cgctgaatgg ctggtacgca tcgacataaa ttttgtcgat caccggcgac
    18121 ataataaaaa aggtcaaaaa cagcgccaac cccagcaata cctggttagg tggcgcggag
    18181 ggtgttccca gcgcgttacg caataaacca aaaacaataa tgatgcgggt gaaactggtc
    18241 atcatcagta aaattgccgg aataaacgtc aacgaggtga tgaacaccag cgtctgcacc
    18301 gggagcgacc agctttgtcc accgccaggc agcggctggc tggtgatacc cggcagttgc
    18361 gcgaaggcga ggggcgtaat cagccagaga aggacagggg cgacagacaa caaacgacgc
    18421 atcaggatct cccgctacgc ttaagcaagt ttttcatgac cgactgaaaa tcgggctgcg
    18481 gtatctcttc cgttggtgct gaagggggaa gtttatgcag cagattgatt tgccctgcgg
    18541 taacgccgag caccagccgt gcatcttcca catcgaccac cacaacccgt tcacgcgcgc
    18601 ccagcgaagc actggcgcta attttcagac cgttaacgcc agtgcgttta ggggcaaatc
    18661 ccaaccgttt taccagccag gcagcagcga ggatcagggc aataatggcg atgagtgcgc
    18721 cgctcacctg cagcagtggc gcagcagaaa ccggcgcgga agattgcaca gtagcgtggt
    18781 tattcatcac taacggctca ggcggcgcat tcgctcagac ggagtaatga tatcggtgat
    18841 ccgcacgcca tatttatcgg caacgaccac cacttcgccc tgggcaatta aataaccgtt
    18901 gatcagaata tccagtggtt cgcccgccag accgtccagc gccacgacgg acccttgcgt
    18961 cagacgcaac agctctttga tggtcatccg cgtacggccc agctcgacgg tcagcttgac
    19021 cggaatatcc ataatcaggt cgatatcctg caacgttccg ctgacatcac caccgccaaa
    19081 ttgctggaac accgcgtcgg cagcgctttt gctgctggtt gatttttgtt cgctcaaagc
    19141 ttcagcccac agatcgtcca ttgcgccgtt gttgtcatcg gccggattat tcatgtcact
    19201 catttgggct gttcctcgtt cagagaattt aaaatcgggt taatcaaatg ttctatccgt
    19261 aacgcatact gaccgttgag ggtgccgtac tgactggtca gtaccggaac gccgtcaaca
    19321 tgagcgatga tgcgatcggg tttttctatc ggcaggacgt cgccgggttt cagttttaaa
    19381 atctgcgaca ggcgtagcga gatatcggca aagttggcga ccagctccag ctgtgaatgc
    19441 tgcacctggc gcaccaggtt atcgcgccag ttctgatctt cattacgcga gttttccagc
    19501 ggcgggttaa ccaacaattc ccgtagcggc tcgatcatgc tgaatggcag gcagatatta
    19561 aattcgccgg tcaggttgcc aatctccaca tggaacggcg tgttaaccac aatgtcgttc
    19621 ggcgaggtgg tgatattggt aaatttcacc tgcatttccg aacgcacgta ctcaacttcc
    19681 agcggattaa tcgccttcca ggcgtcgcta tagccttcaa gcgccagttt caacatgcgg
    19741 ttgatgacgc gctgttcggt atgggtaaac tcgcgacctt ccactttggt cgggaagcgt
    19801 ccatcgccgc caaacaggtt atccacggcg ataaacacca gactcggcga gaacaccacc
    19861 agccctgtgc cgcgcagcgg tttcagatgg ataaggttca ggttggtcgg caccggcagg
    19921 ttgcgggcaa attcatggta cggctgaatg cggatggccc cgacggtaat atccgggcta
    19981 cggcgcagca ggttgaacag ccccatacga aaatggcgag caaagcgctc attaatgatt
    20041 tccagcgcct gcaaacgttc gcgcacaacc cgtcgttggg tattcggatc gtacggacga
    20101 atgtcacttt cgccgctaac actggctgtc ggttcgtctt tgacttcgct gtcgccattc
    20161 aacagcgcat caatttcagc ttgagaaaga atactatcgc ccatgtcgtt atcgcagaat
    20221 aaaagcggta tacagcacgt cggtgacatc ctgtttcggt tgcccggcaa caagcggggt
    20281 ggaaagtgtg gttttaatct cggcaatcag gtttttcttg ccttcttctg tcgccagtac
    20341 ggcagcatcc tgacgcgaaa acagtaacag caaacggcta cggacttccg gcaaatactc
    20401 actcagccgc gagcgggtag cttcatcttt caggcgcagg gttatgccga tataaagcac
    20461 gcgatccgca tcgcccagat tgaccgtgaa ggtatccagc gcatagaaga ccggtgtggg
    20521 cacgacgcgt tgctgcgctt tgtcgtcagc ggcaacctga tgcgaatgcc agtagctgta
    20581 acctgcgctg gcacaggccg cgagggtaat gaataccaga atcgggatcc aaagcgatcg
    20641 cttgcttttc ttgcttatcg cgtaatcagt catgtgttgc ggtcttcctg tgtcgctact
    20701 gcttatcggc taattatcct gcgtcttgag cgcaacaaag cggggaaaag acgcggatta
    20761 cggtgctacc tctgacgtta ggcgaaaata tcaacgccgc tgttgcctgt tacacgccct
    20821 tgtaaagaga cgggaaccgg aagcgtatcg tcgtcttccc ccgccagagg ttcatggttt
    20881 actgtgcgtt ggctttgctg ttgttgggaa gcggcctgct gctgaccact aaagctttcg
    20941 ccactgatgt tgctttgccc taactgaatg ccactttcgg ccagctgcgt acgcagtacc
    21001 ggcagcgctg cttccagggc ggcgcgtaca tgctgatgcg gtgaaaccat ctggatttgc
    21061 gcctggttat catccacttt gagggagatt tgcacttcac ctaaatcctg cgggtgcaga
    21121 cgcaactctg cactttgttg cccctggcgg gtgaacagcg aaatatgctg gcttaatgat
    21181 tgttgccatt cgtgagaacc cagcggtgca ctcaaaacag gcgcggcgac ggtgggcagt
    21241 ggctgtgtct ggtgtggagt gattagcggg ctggcggcag cggtcaccgg tgaaggtgtg
    21301 ctgatgactt ccgctttact ctgggcttct gctaccagcg gtgttaatgg ctgagctggt
    21361 gtgccggggg catcatcagg ctgtgctgtt gtgagttgcg cagaagtcag ttttgtgaag
    21421 agcgttggtt tctctgccgg taacacggtt gacggcacat cagtcacttt gggcgtattg
    21481 tcaacacccg gcaacatcgc aaaaagggcg ctcaggcttg cggtgacgtc ttcattcaga
    21541 tcatccgctt tttcgtcttt tgtcgtattt ttgtcagcca ccgcagccaa cgccatcgtc
    21601 tgagcggtgg ttaacggtgt tgatgtggat tgttcgtcgt tgatgacagg cggtgtttca
    21661 tccacaggga tcagtaaatt agcttgttgc gcgtcggaaa caatatcgct gatcagcggc
    21721 tcgccttttg tcgtgggctt atctgttgcc accagcaact ggggggccgc tttgtcggta
    21781 gttgtctcgc ctgctaatgc ttcgctcaac aacgcgagaa aatcttgtgc agcatcgctg
    21841 gctttgccgc caggcaatgt ggtggtgtca acgtcggcgg taatcaaggg cgctaagcga
    21901 atcattcagg tttcctcatg gcggcgcgct gggcgaactc atccatcttt ttctgatcga
    21961 ggcggttttc tgcaagcagt gccgccgtgg attgccgttc ctgcagtgtc tgccaggcct
    22021 gcaaacgttg tttcttttct cgccagctgt tcagggcaat gtcaactttc tgcgtccact
    22081 gattaagttg ctggcgatgc tgagtaatgg ctttttccag cgtctggata aactgctgat
    22141 agttgatcca gcggttgctg gtcatcccgg cactcatatc gctgttgagg ttattgcgat
    22201 attcattctg ataatcgatc agcattttga gctgttcttc cgcctgctga cacccgcgac
    22261 gcatttcacc cagcagacgc gcggcatcct ctacctcttt ttctgccaga tctttcaggg
    22321 tcgccagcgc gccatgttct gccatctgcc gttatctcct gggttatgac actgtcggga
    22381 aaatacgctc cagcccctgg agagacgctt cccagtccgc gcgttcaaaa atgccttgtt
    22441 gtaaatagcc ctccagctgc ggccacaggg cgatggcttt atcgagcatc ggatcgctgc
    22501 ctttggcata cgcgccgacg ctaaccagat cgcggttacg ctgaaaactc gacaacagct
    22561 gtttaaaggt gcgcactcgc gcgtaatgtt gctcactgat caacgccgtc attgcgcggc
    22621 tgatcgacgc ttcaatatcg atagccggat agtgcccggc ttccgccagt cggcgagaca
    22681 gcacaatgtg accgtcgagg atcgcccgcg cggagtcggc aatggggtcc tgctggtcat
    22741 cgccttcagt gagcacggta taaaacgcgg taatcgagcc gccgccgcta atgccatttc
    22801 cggcacgctc gaccagtgcc ggtaatttgg caaacaccga cggtggataa cctttggtgg
    22861 cgggtggttc gccaatcgcc agcgcaattt cacgctgggc catcgcgtag cgggtgaggg
    22921 agtccataat cagcaacaca tgctgaccac gatcgcgaaa atcttcggca atgcgcgtgg
    22981 cataggcggc accttgcatt cgcaggagcg gagacacatc cgccggagcg gcaatcacca
    23041 ctgatcgtgc acgcccttcg gcaccgagga tattctcaat aaaatctttt acttcgcgcc
    23101 cacgttcacc aatcaaaccc acgacaatga catcggcgcg ggtgtaacgg gccatcatcc
    23161 ccagcagcac acttttaccg acaccagaac cagcaaacag acccatacgc tgcccgcgcc
    23221 caacggtaag cagggcattg attgggcgca cgccggtgtc cagcacatgt tcaatcggtg
    23281 tacgttgcaa tgggttaaat ggtggggtaa tcagcgcacc ggtttccgtc gtatcggggg
    23341 agggcaggcc atcgagcggt ttaccgctgc cgtccagaac gcgacctaat aacgccggac
    23401 cgagcggcaa ctgcttgccg ctttgcagcc cttctgtcga aatgtttttg gcataaacac
    23461 gcgcgccggg caggacacct tccacttcct ccagcggcat taaaaacagc cgttgaccgt
    23521 taaagccaac gacttcgctt tctacttcgt gcgtttcgct gccgttctgg cgctcaatga
    23581 cacaggttgc gccgagcggc aattgtaatc cggtggcttc cagcaccagc ccggtagcgc
    23641 gagttaatcg cccgtagcga cgtaccgcag gcaactgcgt cattttggct tcaaagttat
    23701 ccagcgtggt tagccagcga gtcaggcgcg tggtcattac accactcctg gtgctgccag
    23761 acggcagagt tcttgccagc gagtggcgac actggcgtcg agatcgcctt catcggcgga
    23821 gactttgcaa ccgccaggat ggagggtggg atcgccccgc aagcgccagc catgcaaact
    23881 taaggttgcg ccgagcatat catccacacg ttgcaggtca tccggatgca cgcgcagctg
    23941 tggtttaccg ctgaataacg gttcttgctg caacagctgt tggatctgtt tgatcagtgc
    24001 cgagttatcc accgttggcg tctgaccgat cacctgacgt gccgcctcca gcgccatctg
    24061 catcaggcgc gaagcaatca cactatcaag cgcatcaagg gtagtttgaa attcgctgac
    24121 cagttgctgc atccgggcat gaattggcgc ttgttgagac ttcgcctctg ccagaccttg
    24181 ttccagcccc tgggccagtc cttcctgata gccctgctta tgaccttgct ggcgaccttc
    24241 ggcaatccca gcctgataac cttgctcgtg ggcctgcatt tgcagttgcg ccagttgctg
    24301 ctcaaggctg ggttcggcct cttcaatgat ggtttcttcc ggctcaacta ttggcacaaa
    24361 ctctgcctgt ggtggcgcga gatcgtccgg cgtccaggtt ttccagggca gattatcaga
    24421 cataggtatc ctcgccgctg ccaattacca tctcgccagt ttcggcaagg cggcgcacaa
    24481 tcagcagaat cgctttctgt tcgttttcca cctgcgacag acgcaccgga ccacggttgg
    24541 cgagatcgtc gcgcagaata tcggcggcac gttgcgacat attgcgcagg aatttctcgc
    24601 gcagtggctg ctcggcccct ttcagcgcga tcaacagcga ttcggaatcc acttcctgca
    24661 acagacgctg gatgctgcga tcgtcgacat ccaccagatt ctcgaacagg aacatctcgt
    24721 cgatgatttt ctgcgccagc tcgccgtcga attcacgcac ggcggtaata acggcttctt
    24781 cctgctgagt tttcatcagg ttgataattt cggctgccgt tctcacgccg cccattttgc
    24841 tgcgcttgag attctgaccg tcgagcaagc cattcagtac ttcggtcagc tccgccagcg
    24901 cggctggctg cacgccgcca aaggtggcga tacgcaacat cacgtcgtgg cgcagacgtt
    24961 catcgaacaa cgccagaata tcggcggcct gggcgcgctt cagatgcacc agaatggtgg
    25021 cgataatttg cggatgctca tcgcgaatca gatcggcggc gctctgcggc tccataaagt
    25081 tgagcgtttc aataccgctg gcggtatcgc gagtttcgag aatatcttcc agcaggctgg
    25141 cggcacgttc ctcacccaga gctttgacca ataccgaacg cagataatcg ttggcgttga
    25201 tattcagtgc ggcaaactgt tcagcttctt gctcaaactc tgccagcaca tcggttagct
    25261 gcttgttgga gatctgcgtg acgttcgcca ttgcagcgct cagggtttgc acttcgcgct
    25321 gggagaggtg cttgaacacc tctgccgccc ggtcttcgcc aatggtcatc agcaggatga
    25381 cgcttttatc tgtgcctgtc aggttactca tgatcgttat ttatccactg gcgaatgacc
    25441 agcgccacca cgcgcggatc gttatcagac atttcacgga tacgctggct catgacttct
    25501 gcacccagcc gttggttagc gcgccgttgt tgtagttgtt cgtctttgct caggcggact
    25561 tccaccgcat cttccacttc ctcgcgggcc tgcgcctgtt gctgtacagc tttcatcgcc
    25621 tcagcgcgac gtgttagctg cggacgtacc gctttccgcc acagcagcca cgccaccagc
    25681 agtaccagca accagcgacc ggcagcaatt aactgatcga taaacgcttg ctgttgccag
    25741 aatggcagtt ctccgccgct ttcgtcactg ctattgaacg gcgagttaac gacattgagc
    25801 gaatcaccgc gtttttcaga aaagcccatc gcctcgcggg tcagatcttc aatttgcttc
    25861 atctgttcgt tgctgagagg caacggtttg ccatctggca aggttttgta attcaccaca
    25921 accgcgactg acagacgttg cacatcgccc acgttcattt tggtatgacg aatggtgcga
    25981 tcgacttcgt agttactggt ttcattccgc tgtgtgctac gcggcccact attgctggtg
    26041 gtgctcgcct gctgctggcg gttattttga tttgccggag gcgtgctgat tggcgcgtta
    26101 ttcgcaggtg ccggttgatt cgacaacgcc cccggtacgc cgcccggata accggaaccg
    26161 ctttgctcgc tctcattaag ctggcgtgaa cgaagcgccg cctgagattc atcaccatta
    26221 gggcgatact gttcttccgt ttgttcttta ctggcgaagt ccaactgcgc cgtaacctgg
    26281 gcgtgaatat taccgttgcc gacaataggc gacaggatcg cttcaatacg ccgctgaata
    26341 cggccttcga catcgctggc atatttcaac tgagcgtcat taagatcgcg cccgctggtg
    26401 ttggactggg ttaacagatg tccgccctga tccaccagcg tgacgttccc cggcggcaga
    26461 ccagccaccg cgctggaaac cagatgcaca atggcgctaa tttgcccttc atcgagtgcg
    26521 cggccgggta acagatttac cgtcaccgat gcagaagggg atttttgttc acggacgaat
    26581 aaagacggtt tcggcattgc cagatgtacg cgcgcccctt ttaccgggcc gatagtttcg
    26641 atggtacgag aaagctcgcc ttccagcgcc cgctgataat tcacctgttc gctgaactgg
    26701 ctgataccaa acttttcctg atcgagcagt tcgaaaccga ccgcgccgcc tttgggcaaa
    26761 ccttgttgcg ccaggcgcag acgcagctcg tgaactttat ctgccggaac ttcaatagcg
    26821 ccgctggctt cgctgaagcg gtaaggaata ttcatttgcg tcagttggct gacaattgcg
    26881 ccaccatcct gatcggaaag attgctgaat aatgtgcggt agtcgggggc tttcgcccac
    26941 aggatcagtg cgaccatgac cgccactgcc gcggaaccgg caacaatcaa tggaattttc
    27001 ggattcgcac gcaggcgatt aagccactca agagattttg tctgggctgc agtcgcattc
    27061 atcgcgcacc tcgtggctga ttatggacgg cgttattggc aaagtggaac aagactcact
    27121 cccgggtcag caaactcgaa aaattgacgc tgccattatt tgtgctttcg tttttttcta
    27181 tccgccaata aacccgtttt tttgttgcta tttagcgcct ttgtcttatt gacttactgg
    27241 taggctttgc taccagaaat tatccaggag acgaaaatgt cagcgataca ggggattgaa
    27301 ggggttatca gccagttaca ggctacggcg atgagtgcgc gtgcgcagga ttcactgccg
    27361 caaccgacca ttagttttgc cgggcagctg cacgccgcgc tcgatcgcat tagcgataca
    27421 caaacagctg cccgcacgca ggcagaaaaa ttcaccctcg gtgaacccgg cgtggcgtta
    27481 aacgatgtga tgaccgatat gcaaaaagcc tcagtttcta tgcaaatggg gattcaggtg
    27541 cgtaataagc tggtggcggc gtatcaggaa gtgatgtcca tgcaggtgta ggttttgtaa
    27601 cctgttgtta attacttcca atctcaccgc ttggggcatg gatggggcaa actcactcaa
    27661 tttctggttt aaaatggata cctgatcctg aaagttttct gccatccacg atccgtaaac
    27721 gcgataaccc ctctgaacat cagagtgtct cttttaacac tcatcaccac gtcggatgtt
    27781 gctacgaccg cagacgcaac agtaacgttt gaataattta atctttgttg atgtttttat
    27841 tcatattccg ggctagggaa ataatcaggc gtaattgtgt cactggaata gaggtttctt
    27901 gtggcgcatg tggaaggagc gtctactttg tctaatagag atttcttatg acgcagtatt
    27961 attgatcaat agtctcctgg cttatcaaac cggttgaatt aactccgcac cctggtttct
    28021 aacattaccc accgcgcgcg ataccgggtg ccaggagaac tggtttgctg gaacacagcc
    28081 actggccgcg atttctgagg cttctttacc gctaatctct tgccgcatcc attcccgcgc
    28141 agcttctggc gacagtacca gtgggcggcg gtcatgaata tctaccagac cttgatctgc
    28201 cgcagctgtg acgatcaaaa atccttcggc ttcgtcacca cgctcaaatg gtgtgctacc
    28261 tatcgcggcc ataaaaatag gttgtccatc agcacgatag ataaaataag gctgcttttt
    28321 gtcgccttct tttttccact caaaccagcc atcggcaaaa cagattgccc gaccatgttg
    28381 ccagagcggt ttaaacatac gactggttgc cgcagtttct acgcgggcgt taatcagcgg
    28441 cggtttatcc caccatccgg gagcatatcc ccagaaaacc ggatccagat gaaggtgttc
    28501 atcacgttca ctgagcagta ggactttggt tcccggcgcg acgttgtatc tgccaatggg
    28561 ttcgggatca tagggaatat cgcgttcaat atcttccgcg agaagcgcaa ggtaatcttc
    28621 acgcgtttgg gattgggcaa agcgtccaca catagaaacc tcctgagatc atactgaaaa
    28681 gaataggaga gggggtgggt aaaatctatg tgatacaaag gtaacgatgt tcccgctgtg
    28741 ggtcacagcg ggaatgaggg attacttctg aattaaataa cgaatggtcg gcccgtcttg
    28801 ctgaatatcc agcaccgtat agccgtgatt acgcgcatcc agtggaatat tattgatcga
    28861 ctgcggacag tcgctcacca cttccaggat ttcccctttt tttaactgcg gcatcgcctc
    28921 aagggttgcg actgccggat aagggcaggg ttcacccacc atatcaaggc ggtaatcagg
    28981 aacgatattt ttcatgcgat ctccttagca gtctgcggtg ccgcacggcg gaagaagcgt
    29041 ttttcccagc cgataatcaa catcagcgca gcaaacaaca gcaaatatgt caccagcagg
    29101 ccacccaccg gaccaaaggt tttcagcagg ttgattttgt cccagtcggt ggccagcgcc
    29161 ggagcgaaat catcccagta atacgccaga atcgttgagc cgatcacatt gcccagaccg
    29221 acccaccagt agtgcacctg gccttctacc gcgcggtaca tccagccggt ttcgcagccg
    29281 ccagccagca cgatgccaaa accaaacagt aaaccaccaa ttaccgcgtt tggtcccgcc
    29341 cacatgattt tgggttcaac gcctaactgt acgtaactga agatcccgat ggcactcacc
    29401 gccataccaa taatgattgc tttcgccata tgggtacgtc cggtgatcca catatcgcgg
    29461 aacgccgaag taaagcagat ttgcgcacgt tcaatcagta aaccaaagcc gacgccaaac
    29521 agcattgcca gccccagttt gggttggttc atcgctgtga gcagcgccca gcccagcatg
    29581 ccgaaaaaga ccaccatccc gagacgaaaa cgacgccgcg cctgatccgg tttttgcgtc
    29641 agcggtgagg cggcagaaac tttctgcatt ttcacgggaa tacggaagat gggcagcagg
    29701 gtaaagcgcg cgccaaacca tgaaccaatg gcagtggcga tggcaaagaa ccaggcatgc
    29761 agcgagaact gaggaatacc ggtaaagaac gccgccaggt tacagcccat tgccagacgc
    29821 gcgccaaaac cggcgataat gccgccaatg atggcctgca taatgcggat acggctgcgc
    29881 ggcattcgca gtttgacatt gttggcccac agcgctgcgg caaagcagcc gccaaacata
    29941 ccgaggatca tcatcccgtc gatgcgggtt aatggcgatc cttccagatg gataatttta
    30001 aagtaacccc actcttcagc atggacgccg aacagttgca ggagctggcc gccccaacgg
    30061 gtaaattcac ccgtgacagc ccaaaaggtg ccagtaatgc caaaatagta agtagagaga
    30121 atacccgccg cgatgaccgc agggatgggg gcccagaatt taatcaacca ggcgtgtttg
    30181 aattgctgcc atgacatgaa tgtagcctct gaactcagaa ggtttgaaac aagagtcgcg
    30241 aatcatacac catgcaaatt tattttcggg gtaataattc acaaaaataa tagttagatc
    30301 aaatttctct ctgaaccttg aaaggctggc gacatcaggg ctttaatgcc acaatcctct
    30361 tttcttctca tgcaggacga atgatgaaaa aactcgcaat tgcaggcgcg ctcttgttgc
    30421 tggcgggctg tgccgaagtc gaaaactata acaatgtcgt gaaaaccccc gcgccggact
    30481 ggctggcggg ctactggcag accaaagggc cgcaacgtgc gctggtcagc ccggaagcga
    30541 taggcagcct gattgtcact aaagagggcg ataccctgga ttgccgtcag tggcagcggg
    30601 tgattgccgt gccgggtaag ttgacattga tgtctgatga tctcactaac gttaccgtaa
    30661 aacgtgagct gtacgaggtt gagcgtgacg gtaacaccat tgagtacgat ggtatgacga
    30721 tggagcgtgt ggatcgcccg acagcagagt gtgccgccgc gctggataaa gcgccgttac
    30781 cgacgccact gccgtaatga aatctccccg gcttgctgcc ggggatgaat ttaaatcacc
    30841 tcttcgataa cccacacgct aacgctgccg ccgttgcaaa agaaggtcgc ttcgccgttt
    30901 tcgtcggtta ctacactctc ttgccggttc ccaagaaaat cacgccaggt tttattgccg
    30961 taattctctc ccagatgaat ggttttttcg ccatcatccc cgttcgacat gaccaccacg
    31021 cagccgggat attcgtcagt gccactgcgg ctaaaggcaa tgcagttcgg atggtcgaaa
    31081 aataacgtct gtacaccgtg ggcgaaacgc tgacgggcga gaattaactc atcaagctgt
    31141 tcgattattg gcatatctat cggataggtt tgcccgtcac caccgacatc ttcgtaatgc
    31201 gcaccgtaga ggtccggata gaataccgaa ggaacgccat tttcccgcaa caaaattaag
    31261 gcatacgcca gcagtttaaa ccacggttcg actggcgctt cgagggcttg caacggttgg
    31321 gtgtcatggt tggcaacgag ggttacggcg tgaaaaggat cggcttccac taatgtaccc
    31381 gtgaaaatct gcgtcatgtc gtagtcgcgc cccatgcgcg atgcttcatg gaatttcatc
    31441 tgcagcggcg catcgaacag catggttttg ccttccacct gatcaatata cgtttgcagt
    31501 ttatcgactt catgcgacca gtactctgcc acaataaaca gcggctttgg cgcaacttcc
    31561 tgtacgtgtt cgatccactc tttataaaac cacgccggaa tatgtttgac cgcatcaaga
    31621 cgaaaaccgt cgcattgtgt ttgttccatc acccagcgcg cccagtattt aatctcttcc
    31681 gtcacggcat gattgcggaa atcgatattc tcacccatca gataatcgaa attacctaat
    31741 tcatcatcaa cctgatcgtt ccagccttcg ccggtgtagt cgttaacaat tttaaatatg
    31801 ccatcttcat cagggttttc gatatggtcg ataccgctaa aacatttgaa atcccagata
    31861 aactgtgagt attgcccggc acgggcgggg aaggtgtaac gcgtccagcc ttcgcactca
    31921 atgatttctt catcaatttg cgtacggtcg tcagcattta cacgctgcac gcgaatagct
    31981 tctttttcat ccgcgcccat tttgtggttg actaccacgt ccaacagcac cgcaatgtca
    32041 ttacgtttca gggcgtcaat ggcggccagc agttgtactt tatcgccata tttagtaggg
    32101 atgctgcctt tctgatcaaa ctcacctaaa tcaaataaat cataggagtc gtagccgacc
    32161 gaatacccgc ccgatgcgcc tttataggcg ggcggcaacc agaccatatt gataccaata
    32221 tcattaaaac cgtcggcgcg ctcggccagt tcaggccaga gcttaccgcc ttccgggtaa
    32281 taccagtgaa aacattgtaa cagcgtggga ttacgcattc ttcgactcca ttcaagggga
    32341 acattagaag cgtagccgta atcggattat tcgcgagcca tcgactcatt cagattcaaa
    32401 agaggttatc ctgcggagcc agcacaaagc cgccctgatc gccataggca cttaacaccg
    32461 atttttgcac cgatgactga ccgaccagtt tcgccagttc atccatccga atctgtaata
    32521 actgctttac cttgctttcg ttgtcgagaa tcaggcgcag catcgggcgg agctgctcct
    32581 gcatcgtggt ggacgagtca acctcttcag tcaaatgtgc aatctcctgc accgcattaa
    32641 catacgccat ttcgctggcg atgagttcgt cccattgttc ttccgttgcc aggcgtaaca
    32701 tgagctggct tttttcgacg agttgttgcc aggcgaaata taaatgcggt gcatggttca
    32761 tcagactggg tcctgaatca aagaagggga gagtaacgac tctttccagg catcggcaat
    32821 attacgcatt aatgcttcca cttcttcgac tgcggagaca tcgttgcgta aattggcttg
    32881 cagcaagcgc ctgaccatat agctataaag agcaatcaag ttttgggtta attcgtcttt
    32941 gctctcttca tcaagactca cccgcagtcc gttctcaatg atgttgatcg cttttgacaa
    33001 agagacgcct ttgccttgct gattgttgtc ctgcataaac aggcgcgctc taaccagtgc
    33061 gctcagcact ccatcaaata gcatggtgac cagttgctgc tggctggcgc tcattacggc
    33121 gctttcgacg ccaatttgtg cataggcctg ggtgcctttt gccgcgtaca tagtttctcc
    33181 tgaagattat ttcgagttgc tatttgaggt gtcgaactgc tgtgtcagat agctactggt
    33241 ggagttaagc gagttcatta acacatctaa ttgggtgaac tgggctttat agcgcgtcat
    33301 taaggtatcg atacgctcac tggctgcgtt gtactggtcg gtcaaattat tcagcgtttt
    33361 gctgacgcca tcttttgcgg cctggattat cccggtggaa gagagccaac tggtcaggtt
    33421 gctactaatg ccggtagtta cccctgtact tttaccgtca cctataaaca tttcaccgat
    33481 gccagcggca tcttttttta gtgccgtttg cagtttgtcg gtcgcgattt ccagcttgcc
    33541 ggtactggca tcgctggtaa tgccaatctg tgccagcgtt ttatagtttg aactgctgtg
    33601 agtattggcg agcaaggttt taagctgagt ctggatggta cgcagtgtgg agtcaccaag
    33661 taatgctcca ttactggagt cctgactttc tgctccggca tcgacagcgg tgtatttggt
    33721 taagctactg aatgtatcct gcaatgtgtt ataggcatcc acccatgctt tgactgcatt
    33781 ttcagctttg gatgtgtctt tactgatggt cagtgtctga ttaccggtgg tgacgtcatt
    33841 caggttgagt gtgatatctt ccagcgcgtc gctgatagtg ttactgctgt tttcaatctc
    33901 aacgttattg actgtcagtt tggcgttttg cgcggtgacg ctttcaatca tgccattgct
    33961 gctatcaccc gacgttccgt tgtaacccat aaaactttcc agggcgctgt cgccgctgac
    34021 gctaagtttc atagcgttat cgttgcccgt atcgttagcg gtgatggaca gacgatattc
    34081 gccgtttccc acattgatga tactggcgct gacaccagct ttggcgttgt taatggcatc
    34141 gcgaatgccg gaaagtgacg agttagccgc gctgatatca atcgtgaccg gatctttacc
    34201 gccgccttgc tggatagtca gtacgctatc gctggtggcg atggcagctt tactgtcttt
    34261 ttgactaatt tttgtggtca gcgtttgtgc ctgcgccagt tggctaacgc taatggtgta
    34321 tttcccggca atcgcgctgc ccgtggtggt ggcgctgaaa gccgatgaac tgctggtggt
    34381 tgaagtggcg gcaaataaat cggctttatt cagtgctgtg ttggctgtct ggaaactctc
    34441 tagtgcactt ttcagcgtcc cgtaggcgct cagtttcgcg gtgtaagagc tttgctgttt
    34501 tgatatcggc gtcagggtag atttttctgc cgcctccaga ctgtcaagga tgctgcttaa
    34561 gtccagcccc gacccaaccc caagcgttga aatactagcc atataaattc cttaatcatg
    34621 cgacgtatag aacgaatacc ggggttatcg gcgtaagcgg ggcaaagttt acgatttatt
    34681 ttttggctta atgacacgaa cagcaacgag gaaggggagt atttcgaccg ctagaaaaaa
    34741 attctaaagg ttgtgagtga ccagacgata acagggttga cggcgacgaa gccgaagggt
    34801 ggaagcccaa tacttaaacc gtagacttga aaacaggaaa atgaatcatg gcacaagtca
    34861 ttaataccaa cagcctctcg ctgatcactc agaacaacat caacaaaaac cagtctgcgc
    34921 tgtcgacttc tatcgagcgc ctctcttctg gtctgcgcat taacagcgct aaagatgacg
    34981 ctgcgggcca ggcgattgct aaccgcttca cttctaacat caaaggtctg actcaggccg
    35041 cacgtaacgc caacgacggt atctctctgg cgcagaccac tgaaggcgca ctgtctgaaa
    35101 tcaacaacaa cctgcagcgt gttcgtgaat tgaccgttca ggccactacc ggtactaact
    35161 ctgattctga cctgtcttca atacaggacg aaatcaaatc ccgtctcgat gaaattgacc
    35221 gcgtatccgg tcagactcag ttcaacggcg ttaatgttct ttccaaagat ggttcaatga
    35281 aaattcaggt tggtgcgaat gatggtcaaa ctatctccat cgatctgaag aaaattgatt
    35341 cttcaacttt ggggctgaat ggcttctcag tttctaaaaa ctctcttaat gtcagcaatg
    35401 ctatcacatc tatcccgcaa gccgctggca atgaacctgt tgatgttaac ttcggtgata
    35461 ctgatgagtc tgcagcaatc gcagccaaat tgggggtttc cgatacgtca agcctgtcgc
    35521 tgcacaacat ccttgataaa gatggtaagg caacagctga ttatgttgtt cagtcaggta
    35581 aagacttcta tgctgcttct gttaatgccg cttcaggtaa agtaacctta aacaccattg
    35641 atgttactta tgatgattat gcgaacggtg ttgacgatgc caagcaaaca ggtcagctga
    35701 tcaaagtttc agcagataaa gacggcacag ctcaaggttt cgtcacactt caaggcaaaa
    35761 actattctgc tggtgatgcg gcagacattc ttaagaatgg agcaacagct cttgagttaa
    35821 ctgatctgaa tttaagtgat gttactgata ctaatggtaa ggtaaccaca actgcgactg
    35881 agcaatttga aggtgcttca actgaggatc cgctggcgct tctggataaa gctattgcat
    35941 cagtcgacaa attccggtct tctctaggtg ccgtgcagaa ccgtctcgat tccgctatca
    36001 ccaacctgaa caacaccacc accaacctgt ctgaagcgca gtcccgcatt caggacgccg
    36061 actatgcgac cgaagtgtcc aacatgtcga aagcgcagat catccagcag gcaggtaact
    36121 ccgtgctgtc caaagctaac caggtaccgc agcaggttct gtctctgctg cagggttaat
    36181 cgtcgtaacc tgattaactg agactgacgg caacgccaaa ttgcctgatg cgctgcgctt
    36241 atcaggccta caaggtgaat tgcaatttat tgaatttgca catttttgta ggccggttaa
    36301 ggcgtttacg ccgcatccgg caacatgaag cgcaatttgt cagcaacgtg cttccccgcc
    36361 accggcgggg ttttttctgt ccgcaattta ccggtaaccc ccaaataacc cctcatttca
    36421 cccactaatc gtccgattaa aaaccctgca gaaacggata atcatgccga taactcatat
    36481 aacgcagggc tgtttatcgt gaattcactc tataccgctg aaggtgtaat ggataaacac
    36541 tcgctgtggc agcgttatgt cccgctggtg cgtcacgaag cattgcgcct gcaggttcga
    36601 ctgcccgcga gcgtggaact tgacgatctg ctacaggcgg gcggcattgg gttacttaat
    36661 gccgtcgaac gctatgacgc cctacaagga acggcattta caacttacgc agtgcagcgt
    36721 atccgtggcg ctatgctgga tgaacttcgc agccgtgact gggtgccgcg cagcgtgcga
    36781 cgcaacgcgc gtgaagtggc acaggcaata gggcaactgg agcaggaact tggccgcaac
    36841 gccacggaaa ctgaggtagc ggaacgttta gggatcgata ttgccgatta tcgccaaatg
    36901 ttgctcgaca ccaataacag ccagctcttc tcctacgatg agtggcgcga agagcacggc
    36961 gatagcatcg aactggttac tgatgatcat cagcgagaaa acccgctaca acaactgctg
    37021 gacagtaatc tgcgccagcg ggtgatggaa gctatcgaaa cgttgccgga gcgcgaaaaa
    37081 ctggtattaa ccctctatta ccaggaagag ctgaatctca aagagattgg cgcggtgctg
    37141 gaggtcgggg aatcgcgggt cagtcagtta cacagccagg ccattaaacg gttacgcact
    37201 aaactgggta agttataacg tcagtaaatg ccgcacttta attttgacta ccaggagttc
    37261 ttaatgatgg tgcagcacct gaaaagacgg ccattaagcc gctatcttaa agactttaaa
    37321 cacagccaga cccattgcgc gcattgccgt aaattactcg atcgcattac cttagttcgc
    37381 gacggcaaaa tagtgaataa aatcgagatt tcccgcctgg acacgctgct tgatgaaaat
    37441 ggctggcaag tggaacaaca atcatgggcg gcattgtgcc gattttgcgg tgatttacat
    37501 tgcaaaacgc agagtaattt tttcgatatt atcggcttta agcaatttct ttttgagcaa
    37561 actgaaatga gccctggtac ggtgcgtgaa tatgtcgttc gtctgcgccg tttggggaat
    37621 catctacacg agcaaaatat ttccctcgct cagctgcagg acggtttcct tgatgaaatc
    37681 ctcgccccgt ggctgcccac caccagcacc aacaattacc gcatcgcgtt acggaagtat
    37741 caacactatc agcgtcaaac gtgtaccgga cttgtgcaga aatcctccag ccaacctgct
    37801 tctgatatat attaaataag aataagatgt agcggagttg tttttgtgtt tacaaacaat
    37861 ggctctacac tgcaaacaga cataacaaca ttcggggtaa atatgaaatt agcacatctg
    37921 ggacgtcagg cattgatggg tgtgatggcc gtggcgctgg ttgcgggtat gagcgttaaa
    37981 agttttgcag atgaaggtct gcttaataaa gtcaaagagc gcggcacgct gctggtaggg
    38041 ctggaaggaa cttatccgcc gttcagtttt cagggggatg acggcaaatt aaccggtttt
    38101 gaagtggaat ttgcccaaca gctggcaaaa catcttggcg ttgaggcgtc actaaaaccg
    38161 accaaatggg acggtatgct ggcgtcgctg gactctaaac gcattgatgt ggtgattaac
    38221 caggtcacca tttctgacga gcgcaagaaa aaatacgatt tctctacccc gtacaccatt
    38281 tctggtattc aggcgctggt gaaaaaaggc aacgaaggca ccattaaaac ggctgatgat
    38341 ctgaaaggca aaaaagtggg tgtcggtctg ggcaccaact atgaagagtg gctgcgtcag
    38401 aatgttcagg gcgtcgatgt gcgtacctat gatgatgacc cgaccaaata tcaggatctg
    38461 cgcgtaggcc gtatcgatgc gatcctcgtt gatcgtctgg cggcgctgga tctggtgaag
    38521 aaaaccaacg atacgctggc agtaaccggt gaagcattct cccgtcagga gtctggcgtg
    38581 gcgctgcgta aaggaaatga agacctgctg aaagcggtga atgatgcaat tgcggaaatg
    38641 caaaaagacg gcactctgca agctctttcc gaaaaatggt ttggtgctga tgtgaccaaa
    38701 taatcagcat aatgacaaaa aagggcgctt tcactagcgc cttttttatt tacgcgtttt
    38761 cagcgtgcat aataagaaaa tcacaacaac acacatcgga ggctgttatg ccactgcata
    38821 atttaacccg ttttccacgg ctggagttta tcggcgcgcc aacgccgctc gaatatctgc
    38881 cgcgcttttc tgattatctt ggacgggaaa ttttcatcaa acgggatgac gtcaccccca
    38941 tggcaatggg cggcaataaa ttacgtaagc tggaatttct cgcggcagat gctctgcgtg
    39001 aaggtgccga tacgctgatt actgccgggg cgatccagtc taaccatgtg cgccagactg
    39061 ccgcagtcgc tgcgaaactc ggtctgcact gcgtggcgct gctggaaaat cctattggta
    39121 cgaccgcgga aaactattta accaacggca atcgtttgtt gctggatctg ttcaataccc
    39181 agattgaaat gtgcgacgca ctgaccgatc ccaatgccca actggaagag ctggcgacgc
    39241 gagtcgaagc acaaggcttt cgcccgtatg tcattccggt tggcggttct aatgctcttg
    39301 gcgcgctggg ttatgtggag agtgcgctgg aaatcgcgca acagtgtgaa ggggcggtta
    39361 atatttcgtc ggtggtagtc gcatcgggca gtgccggaac tcacgccgga ctggctgttg
    39421 ggctggaaca cctgatgcct gaaagcgaac tgattggcgt gaccgtgtcg cgttccgttg
    39481 ccgatcaatt gccgaaagtg gttaacctac aacaggcgat tgcgaaagaa ctggagctga
    39541 ccgcatcagc ggaaattata ctctgggatg actattttgc acctggctac ggcgtgccga
    39601 acgacgaagg catggaagca gtgaaattgc tggcgcggct ggaaggcatt ctgcttgatc
    39661 ctgtgtatac cggaaaagcg atggcggggc tgattgacgg tatcagtcag aaacgcttca
    39721 aagatgaagg gccgattctg tttattcata ccggcggcgc gcctgcgctg ttcgcctatc
    39781 atccccacgt ttagctccgg gaacgctcat gcaagaaagt atacaactgg ttattgattc
    39841 cttgccgttc ctgttgaaag gggctgggta tactctgcaa ctcagtatcg gcggcatgtt
    39901 ttttggctta ctactcggat ttattctcgc gctgatgcgc ctgtcgccaa tctggcccgt
    39961 gcgctggctg gcgcgttttt atatctctat ttttcgtggt acgccgctca tcgcacaact
    40021 gtttatgatc tattacggcc tgccgcagtt tggtatcgaa ttagatccca ttccgtcagc
    40081 aatgattggt ctgtcgctga acactgccgc ctatgccgcc gaaacgctgc gcgccgccat
    40141 ttcttctatt gataaaggtc agtgggaagc cgctgccagt attggtatga cgccgtggca
    40201 gaccatgcgt cgcgcgattt tgccacaggc ggcgcgggtg gcgttgccgc cgctgtcgaa
    40261 cagctttatc agtctggtaa aagatacgtc gctggccgcg acaatccagg tgccggagct
    40321 gttccgtcag gcgcagttga ttacctcgcg cacgctggag gtgttcacca tgtacctggc
    40381 ggcttcgctc atctactgga tcatggcgac tgtgttatcg acgttgcaga accattttga
    40441 gaatcaactt aatcgccagg agagagaacc aaaatgagtg ccattgaagt taagaacctg
    40501 gtgaaaaaat ttcacggtca gacggtgctg cacggtatcg acctcgaggt aaagcctggc
    40561 gaagtggtgg caattatcgg tccgagtggt tccggcaaaa ccacgttgct acgcagcata
    40621 aatctgctgg aacaaccgga agcgggaacg atcaccgttg gcgatatcac tattgatact
    40681 gcacgttcat taagtcagca aaaatcgctg attcgccagt tgcgtcagca cgtcgggttt
    40741 gtcttccaga actttaattt gtttccgcat cgtacggtgc tggaaaacat tattgaaggg
    40801 ccggtgatcg tcaaaggcga accgaaagaa gaggccacgg cgcgcgctcg cgagctgctg
    40861 gcgaaagttg ggctggccgg taaagaaacc agctatccac gtcgtttgtc tggcggtcaa
    40921 cagcagcgtg tagcgattgc gcgtgcgctg gcaatgcgtc ctgaggtgat tttgtttgac
    40981 gagccaacgt cagcgctgga tccagagctg gtgggcgaag tcctgaacac aatccgccag
    41041 ctggcgcagg aaaagcgcac gatggtgatt gtgacgcacg aaatgagctt tgcccgggat
    41101 gttgcggacc gggcgatttt tatggaccag gggcggatag tcgagcaggg ggcagcaaaa
    41161 gcgttatttg ctgatcccca gcagcctcgc acccgccagt tcctcgagaa gtttctgctg
    41221 caataataag aaaaaatcag ccccgacgat tcacctgtcg gggcgggacg ccatttcaag
    41281 cctgataaaa ctgcttaaca aatcagcata actcattaat aacataagag aatgctatgg
    41341 cttgcaaaag taattcattg cctgaataat ataaattata tataaatctt atttatgtga
    41401 tagtttgaat tatcattata aatgatactc actctcaggg gcgttgcggt ttactatgca
    41461 ggatacggat tttttcagct ggcgtcgcac gatgctgttg cgttttcaga ggatggagac
    41521 cgcagaagag gtctaccatg aaattgagct tcaggctcag cagctggagt acgattacta
    41581 ttcgttatgt gtccgccacc cggtaccatt cactcgacct aaagtggctt tctacaccaa
    41641 ttaccctgag gcgtgggtta gttattatca ggcaaaaaac tttctcgcaa ttgatccggt
    41701 gctgaaccct gaaaacttta gtcagggcca tctaatgtgg aatgatgact tattcaacga
    41761 agcacagccg ttatgggaag ccgcgcgcgc acatggttta cgccgcggtg tcactcagta
    41821 tttaatgctg ccaaaccggg cgctgggctt tttgtccttt tcccgttgca gcgcgcgcga
    41881 aatacccatt cttagtgatg aactgcaatt aaaaatgcag ttactggtgc gcgaaagtct
    41941 gatggctctg atgcgtttaa atgatgaaat agtgatgacg ccagagatga atttcagcaa
    42001 gcgcgaaaaa gaaattttga aatggacggc ggaagggaag acatcagcag agatagcgat
    42061 gattttgtca atctctgaga atacggtcaa tttccatcag aaaaacatgc agaaaaaaat
    42121 caatgcacca aataagaccc aggtggcctg ttacgcggcc gctactggct taatttgatc
    42181 tcttttctgt tctgcgtgcc agatgcaaaa accggctgaa aggcacgctc tcagccggtt
    42241 ttatattact gacggtaggc ttgtttaatt tgcttaaccg ttttggaaaa tactgcagcc
    42301 tgggtttcgt cttcgatcag cgcaagctgt ttttccatct ttaacatcac gcggcccgcg
    42361 tcagcttgtc ccatcgcctg cagcatcagc gtcagcatcg ctttcaggca ggagacttca
    42421 tttgccagtt cttgattatt ctcggcagta gaaaaatcag gcgtactcat ttattttcct
    42481 cgtcatgttg caatgaaaat ttgcggtgaa aaatgttaag gcggcggagt ataccataag
    42541 ctttgctaaa aatagcagtg gttgtttttt gagcgtgata tcggcagtgc tataaaatac
    42601 gttaatataa tgaccaataa atatttttat catgaatgtt ttttgcccgt attgttcggg
    42661 ttaattaatg ttacatattc agcgggctga ttttcatttt tgctgaataa aggtaatttt
    42721 tgtcacattt cgtcgcaggg cttactgtga aacgatccgg taagccgttg gtgacgggcg
    42781 tgaccataac tgtggacaat cgaattgaca aaaacgagag aaaaatcgaa tacccaccat
    42841 ttttaacgtt tcaaagttgc aataaaaacc gctaatatac gaatgactaa ctatcagtag
    42901 cgttatccct atttctggag atattccttt gatcaacgtt ctacttgttg atgaccacga
    42961 actggtgcgc gcagggatac gacgcattct ggaagatata aagggtatta aagtcgtcgg
    43021 tgaggcatcg tgcggtgaag acgccgttaa gtggtgccgg acaaatgccg ttgacgtggt
    43081 gctaatggac atgagtatgc cgggcattgg cggtcttgag gcgacgcgta aaatcgcgcg
    43141 ttccacagct gatgtcaaaa tcatcatgct taccgtccat acagaaaacc ctttaccagc
    43201 taaagtcatg caggccggtg ctgcgggcta cctcagcaaa ggcgcggctc cgcaggaagt
    43261 cgtgagtgcg attcgttctg tctattcagg gcagcgttac attgcttctg acatcgctca
    43321 acaaatggcg ttaagccaga tcgaaccaga aaaaacagaa agcccatttg ccagtttgtc
    43381 tgaacgtgaa ttgcagatta tgctgatgat caccaagggc cagaaggtca atgagatctc
    43441 agaacagctc aatctcagtc cgaaaacggt gaacagctac cgctatcgta tgttcagtaa
    43501 actaaacatt catggcgatg ttgagctgac tcacctggca attcgccatg gtctgtgtaa
    43561 tgcggagaca ttatcaagtc agtgagtgat cagtttgacg caaaagcgtt tttaaaaacc
    43621 gtaaccagcc agccaggcgt ttatcgcatg tacgatgctg gtggtacggt tatctatatc
    43681 ggcaaagcga aagacctgaa aaaacggctt tccagctatt tccgtagcaa cctcgcttcg
    43741 cgcaaaaccg aagcgctggt cgcccagatt cagcaaattg atgtaacggt tactcataca
    43801 gaaaccgaag cgctgttgct ggaacacaac tacatcaaac tctatcagcc gcgttacaac
    43861 gttttgctac gcgatgataa atcttatcct tttatcttcc tgagtggcga tacccacccg
    43921 cgtctggcga tgcatcgtgg tgcgaagcat gccaaaggtg aatatttcgg cccgttcccg
    43981 aatggctatg ccgtacgtga aactctggcg ctactgcaaa agattttccc cattcgccag
    44041 tgcgaaaata gtgtttatcg caatcgctcg cgtccgtgtc tgcaatatca gatagggcgc
    44101 tgtctgggac cgtgcgttga aggactggtg agtgaagaag aatacgctca gcaggtcgag
    44161 tatgtgcgcc tgtttttgtc tggcaaagat gatcaggtgc ttacgcaact cattagccgt
    44221 atggaaactg ccagtcagaa tctggagttt gaagaagcgg cacgtattcg cgaccaaatt
    44281 caggcggtgc gacgcgtcac cgaaaaacaa ttcgtttcca ataccggcga cgaccttgac
    44341 gttattggtg tggcgttcga tgcgggcatg gcttgtgtcc acgtattgtt cattcgtcag
    44401 ggcaaagtgc tcggcagccg cagctatttc ccgaaagtgc ctggcggtac ggaactgagc
    44461 gaggtggtag aaaccttcgt aggtcagttc tatttacaag gcagccagat gcgcacctta
    44521 ccgggtgaga tcctgctcga ttttaatctt agcgataaaa cgctgctcgc cgattccctt
    44581 tcagaactgg cgggacgcaa gattaatgtt caaaccaaac ctcgcggcga tagggcgcgt
    44641 tatctgaaac tcgcgcgcac caatgcggcg acggccttaa ccagcaaact ttcgcagcaa
    44701 tctaccgttc accagcggct gaccgcgctt gccagcgtgt tgaaattgcc ggaagtgaag
    44761 cggatggagt gctttgacat cagccatacc atgggcgaac aaaccgtcgc ttcctgtgtg
    44821 gtgtttgatg ctaacggccc gctgcgtgcg gagtatcggc gctataacat tacaggcatc
    44881 acgccgggcg atgattatgc ggcgatgaat caggtgctgc gtcggcgtta tggtaaagcc
    44941 attgacgaca gtaagatccc ggatgtgatc cttatcgacg gcggcaaagg ccagcttgcg
    45001 caggcgaaaa atgtcttcgc cgaactggat gtctcatggg ataaaaatca tccgctgcta
    45061 cttggcgttg ccaaaggtgc agatcgtaag gctggactgg aaacgctgtt ctttgagccg
    45121 gaaggtgagg gatttagttt gccgccagat tcacccgcgc tgcatgttat ccagcatatt
    45181 cgcgatgaat cacatgatca cgcgattggc gggcaccgta aaaaacgggc gaaagtcaaa
    45241 aataccagtt ccctggaaac cattgaaggc gtcgggccaa aacgtcggca aatgttgttg
    45301 aaatatatgg gcggtttgca aggtttacgt aacgccagcg tcgaggaaat tgcaaaagtg
    45361 ccgggtattt cgcaaggtct ggcagaaaag atcttctggt cgttgaaaca ttgatgtctc
    45421 tgtagcaaca taggggtaat cttactgaca acagatagtt acccgtcatt atgcaattta
    45481 atatccctac gttgcttaca ctgttccgtg tcatccttat cccattcttt gtattggtct
    45541 tttatctgcc tgtcacctgg tcgccgtttg ccgccgcgct cattttctgc gtcgcggcgg
    45601 tgactgactg gttcgatggt tttctggcac gccgctggaa ccagagtacc cggtttggtg
    45661 ctttccttga ccctgtagca gataaagttc tcgtggctat cgccatggtg ctggtaaccg
    45721 agcattatca cagctggtgg gtgaccctac cggcggcaac gatgatcgcc cgtgaaatta
    45781 ttatttctgc gctacgcgaa tggatggcgg agttgggtaa acgcagtagc gtggctgtct
    45841 cctggattgg gaaagtgaaa accactgccc agatggtggc gttggcctgg ctgctgtggc
    45901 gtccgaacat ttgggttgag tacgccggta ttgcactttt ctttgtggct gcggtactga
    45961 ctctgtggtc aatgttgcaa tatttgagcg ctgcgcgtgc agatttgctt gatcagtgat
    46021 cgtttcggcg caattttcag caaacgatca aaagtggtga aaaatatcgt tgactcatcg
    46081 cgccaggtaa gtagaatgca acgcatcgaa cggcggcact gattgccaga cgataataaa
    46141 atcaagtgat taactgattg cttgatgaat gcgggaatag ctcagttggt agagcacgac
    46201 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccagttt aaaagacatc
    46261 ggcctcaagc ggatgtctgg ctgaaaggcc tgaagaattt ggcgcgttaa caaagcggtt
    46321 atgtagcgga ttgcaaatcc gtctagtccg gttcgactcc ggaacgcgcc tccactttct
    46381 tcccgagccc ggatggtgga atcggtagac acaaggg
//
LOCUS       DAFATA010000022        44151 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFATA010000022 DAFATA010000000
VERSION     DAFATA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.022"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(59..724)
                     /gene="yieH"
                     /locus_tag="LGJ20_001705"
     CDS             complement(59..724)
                     /gene="yieH"
                     /locus_tag="LGJ20_001705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBK2385775.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            891..2228
                     /gene="adeP"
                     /locus_tag="LGJ20_001706"
     CDS             891..2228
                     /gene="adeP"
                     /locus_tag="LGJ20_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBK2385776.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            complement(2282..2848)
                     /gene="yieF"
                     /locus_tag="LGJ20_001707"
                     /gene_synonym="chrR"
     CDS             complement(2282..2848)
                     /gene="yieF"
                     /locus_tag="LGJ20_001707"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBK2385777.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(2870..3619)
                     /locus_tag="LGJ20_001708"
     CDS             complement(2870..3619)
                     /locus_tag="LGJ20_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBK2385778.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            complement(3776..4735)
                     /gene="yidZ"
                     /locus_tag="LGJ20_001709"
     CDS             complement(3776..4735)
                     /gene="yidZ"
                     /locus_tag="LGJ20_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBK2385779.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            complement(4710..5885)
                     /gene="mdtL"
                     /locus_tag="LGJ20_001710"
     CDS             complement(4710..5885)
                     /gene="mdtL"
                     /locus_tag="LGJ20_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBK2385780.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(6018..7265)
                     /gene="tnaB"
                     /locus_tag="LGJ20_001711"
     CDS             complement(6018..7265)
                     /gene="tnaB"
                     /locus_tag="LGJ20_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBK2385781.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(7356..8771)
                     /gene="tnaA"
                     /locus_tag="LGJ20_001712"
     CDS             complement(7356..8771)
                     /gene="tnaA"
                     /locus_tag="LGJ20_001712"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBK2385782.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(8992..9066)
                     /gene="tnaC"
                     /locus_tag="LGJ20_001713"
     CDS             complement(8992..9066)
                     /gene="tnaC"
                     /locus_tag="LGJ20_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBK2385783.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            complement(9309..10673)
                     /gene="mnmE"
                     /locus_tag="LGJ20_001714"
     CDS             complement(9309..10673)
                     /gene="mnmE"
                     /locus_tag="LGJ20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK2385784.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(10779..12425)
                     /gene="yidC"
                     /locus_tag="LGJ20_001715"
     CDS             complement(10779..12425)
                     /gene="yidC"
                     /locus_tag="LGJ20_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK2385785.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(12428..12685)
                     /gene="yidD"
                     /locus_tag="LGJ20_001716"
     CDS             complement(12428..12685)
                     /gene="yidD"
                     /locus_tag="LGJ20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK2385786.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(12649..13008)
                     /gene="rnpA"
                     /locus_tag="LGJ20_001717"
     CDS             complement(12649..13008)
                     /gene="rnpA"
                     /locus_tag="LGJ20_001717"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK2385787.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(13025..13165)
                     /gene="rpmH"
                     /locus_tag="LGJ20_001718"
     CDS             complement(13025..13165)
                     /gene="rpmH"
                     /locus_tag="LGJ20_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK2385788.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(13395..13475)
                     /locus_tag="LGJ20_001719"
     CDS             complement(13395..13475)
                     /locus_tag="LGJ20_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385789.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            13772..15175
                     /gene="dnaA"
                     /locus_tag="LGJ20_001720"
     CDS             13772..15175
                     /gene="dnaA"
                     /locus_tag="LGJ20_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK2385790.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            15180..16280
                     /gene="dnaN"
                     /locus_tag="LGJ20_001721"
     CDS             15180..16280
                     /gene="dnaN"
                     /locus_tag="LGJ20_001721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK2385791.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            16280..17353
                     /gene="recF"
                     /locus_tag="LGJ20_001722"
     CDS             16280..17353
                     /gene="recF"
                     /locus_tag="LGJ20_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK2385792.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMVDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            17382..19796
                     /gene="gyrB"
                     /locus_tag="LGJ20_001723"
     CDS             17382..19796
                     /gene="gyrB"
                     /locus_tag="LGJ20_001723"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK2385793.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVRTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            20036..20434
                     /locus_tag="LGJ20_001724"
     CDS             20036..20434
                     /locus_tag="LGJ20_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBK2385794.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            20549..21361
                     /gene="yidA"
                     /locus_tag="LGJ20_001725"
     CDS             20549..21361
                     /gene="yidA"
                     /locus_tag="LGJ20_001725"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK2385795.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(21407..22063)
                     /locus_tag="LGJ20_001726"
     CDS             complement(21407..22063)
                     /locus_tag="LGJ20_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385796.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            22341..23030
                     /gene="dgoR"
                     /locus_tag="LGJ20_001727"
     CDS             22341..23030
                     /gene="dgoR"
                     /locus_tag="LGJ20_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBK2385797.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            23027..23905
                     /gene="dgoK"
                     /locus_tag="LGJ20_001728"
     CDS             23027..23905
                     /gene="dgoK"
                     /locus_tag="LGJ20_001728"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBK2385798.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPC
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            23889..24506
                     /gene="dgoA"
                     /locus_tag="LGJ20_001729"
     CDS             23889..24506
                     /gene="dgoA"
                     /locus_tag="LGJ20_001729"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBK2385799.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            24503..25651
                     /gene="dgoD"
                     /locus_tag="LGJ20_001730"
     CDS             24503..25651
                     /gene="dgoD"
                     /locus_tag="LGJ20_001730"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBK2385800.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            25726..27063
                     /locus_tag="LGJ20_001731"
     CDS             25726..27063
                     /locus_tag="LGJ20_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2385801.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27060..28124)
                     /gene="cbrA"
                     /locus_tag="LGJ20_001732"
     CDS             complement(27060..28124)
                     /gene="cbrA"
                     /locus_tag="LGJ20_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBK2385802.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            28225..29439
                     /locus_tag="LGJ20_001733"
     CDS             28225..29439
                     /locus_tag="LGJ20_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBK2385803.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(29441..29773)
                     /locus_tag="LGJ20_001734"
     CDS             complement(29441..29773)
                     /locus_tag="LGJ20_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBK2385804.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    30013..30085
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            30079..30492
                     /gene="ibpA"
                     /locus_tag="LGJ20_001735"
     CDS             30079..30492
                     /gene="ibpA"
                     /locus_tag="LGJ20_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBK2385805.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            30604..31032
                     /gene="ibpB"
                     /locus_tag="LGJ20_001736"
     CDS             30604..31032
                     /gene="ibpB"
                     /locus_tag="LGJ20_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBK2385806.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            31229..32890
                     /locus_tag="LGJ20_001737"
     CDS             31229..32890
                     /locus_tag="LGJ20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBK2385807.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(32887..33603)
                     /locus_tag="LGJ20_001738"
     CDS             complement(32887..33603)
                     /locus_tag="LGJ20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2385808.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPVIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            33899..35515
                     /locus_tag="LGJ20_001739"
     CDS             33899..35515
                     /locus_tag="LGJ20_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK2385809.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            35515..36153
                     /locus_tag="LGJ20_001740"
     CDS             35515..36153
                     /locus_tag="LGJ20_001740"
                     /inference="COORDINATES: protein motif:HMM:NF014148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385810.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHWWSAISRSFRKG"
     gene            36153..36329
                     /locus_tag="LGJ20_001741"
                     /pseudo
     CDS             36153..36329
                     /locus_tag="LGJ20_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(36326..37219)
                     /locus_tag="LGJ20_001742"
     CDS             complement(36326..37219)
                     /locus_tag="LGJ20_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK2385811.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            37386..39101
                     /locus_tag="LGJ20_001743"
     CDS             37386..39101
                     /locus_tag="LGJ20_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBK2385812.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            39098..40591
                     /locus_tag="LGJ20_001744"
     CDS             39098..40591
                     /locus_tag="LGJ20_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBK2385813.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(40638..41087)
                     /locus_tag="LGJ20_001745"
     CDS             complement(40638..41087)
                     /locus_tag="LGJ20_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385814.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            41197..41544
                     /locus_tag="LGJ20_001746"
     CDS             41197..41544
                     /locus_tag="LGJ20_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBK2385815.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            41534..41896
                     /locus_tag="LGJ20_001747"
     CDS             41534..41896
                     /locus_tag="LGJ20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBK2385816.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            41893..42390
                     /locus_tag="LGJ20_001748"
     CDS             41893..42390
                     /locus_tag="LGJ20_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK2385817.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QLRRDCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(42398..43582)
                     /gene="emrD"
                     /locus_tag="LGJ20_001749"
     CDS             complement(42398..43582)
                     /gene="emrD"
                     /locus_tag="LGJ20_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBK2385818.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(44001..44090)
                     /gene="tisB"
                     /locus_tag="LGJ20_001750"
     CDS             complement(44001..44090)
                     /gene="tisB"
                     /locus_tag="LGJ20_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBK2385819.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
BASE COUNT        10594 a        11226 c        11627 g        10704 t
ORIGIN      
        1 ccctttactt acctttgagc gccaatttaa gcggggtaag tgaagggagt gtgaagaact
       61 atgccgtaat atcccaacca cgcgctttcc acagttccgg taactgcgaa agatgggtaa
      121 aggtggtgac tttcgggtga acgatcggct tattgtgcgg gtcggcgcag aagtagaaca
      181 cttccatacc tgcgtcgata ccagattgcg caccggcaac tgagtcatca accagaatgc
      241 agttttctac atttacattc atcgcttttg ccgcatggaa cattaacgcc gggtctggct
      301 tccagcgctg aatatcgtag ccgctgaaca gtttatccgg gaagtagtgc aacatattca
      361 gcttgcccat agaatgctgc attttgttat ttgggccgtt agataccaca cacattggcg
      421 cagtgatcgc tgacaggagc gctccagccc cttcgatggc ttccagttct gaatcgaaca
      481 gccgagcgac ttctgcacgg taaacgtgtt cagcttctgt tttcgctaac gtaacaccat
      541 gttcaaggga aacaatatcg ataatttcgt acagttttac acctttgaaa cgtttgaata
      601 cctcttcagg atcgagcgta ataccaaatt cctgaaacat cgttacatat gcgcgggagc
      661 aaatgacttc actgtcgacc agcgtaccgt cgcagtcgaa aaataccgct tctatctggg
      721 acatgtcttt cctttatcgt tagtttaacg ttttcgtcat gccaattcgc atgacgcaat
      781 cgttgccgta taagcaaata caatgaaaaa aagttatctt cagccgtcca tattgtctca
      841 ttttggtata cgatagcgac ggattttccc tccttgttcg gaaatagata atgagtcaac
      901 aacacacaac ccaggcttct ggccagggga tgctggaacg cgtgtttaaa ctgcgcgaac
      961 atggcacgac ggcacggacc gaagtgatcg ccggttttac caccttcctg acgatggttt
     1021 acatcgtttt tgttaacccg caaattcttg gcgttgctgg catggatacc agcgccgtct
     1081 tcgttactac ctgtctgatt gctgcattcg gcagtattat gatgggactg tttgctaacc
     1141 tgccagttgc actggcaccc gctatgggcc tgaatgcatt cttcgctttt gtcgttgtac
     1201 aggctatggg cttgccgtgg caagtcggga tgggcgcaat cttctggggc gcgattggtc
     1261 tgctgttact gacgattttc cgcgttcgtt actggatgat tgctaacatt ccggtaagtc
     1321 tgcgtgtggg catcaccagc ggtatcggtc tgttcattgg tatgatggga ctgaagaatg
     1381 caggtgtgat tgtcgctaac ccggaaacgc tggtcagcat cggtaacctg acttctcaca
     1441 gcgtgctgct ggggatcctc ggcttcttca tcattgctat tctggcctcg cgcaacattc
     1501 acgcggcggt gctggtttcc atcgtggtga caacgctgct gggctggatg ctgggtgatg
     1561 tccactacaa tggcatcgtt tctgcgccgc cgagcgtaat gactgtcgtg ggccatgtag
     1621 atttagccgg gtcgtttaac ctcgggctgg caggggtgat tttctctttc atgctggtca
     1681 acctgtttga ctcctccggt acgctgattg gcgtgaccga taaagcaggc ctggcggatg
     1741 agaagggtaa attcccgcgc atgaagcagg cgctgtatgt cgacagtatc tcttccgtga
     1801 ccggttcgtt tatcggtact tcttccgtta cggcttatat tgagtcctct tccggcgtat
     1861 cggttggcgg tcgtaccggt ctgacggcag tggttgttgg tctgctgttc ctgctggtta
     1921 tctttctgtc gccgctggcg ggaatggtgc caggctacgc tgcagctggc gcgttgatct
     1981 acgttggcgt gttgatgacc tcaagtcttg ctcgcgtgaa ctggcaggat cttactgaat
     2041 ctgttccggc gtttattacc gccgtgatga tgccgttcag cttttcgatt accgaaggta
     2101 ttgcgctggg ctttatctcc tactgcgtga tgaagattgg taccgggcgt ctgcgtgacc
     2161 ttagcccgtg cgtaatcatc gttgcgctga tgtttatcct gaagattgta tttatcgacg
     2221 ctcactaaaa tcgatagcgc attaaaaacc cgccatgcat gattactggc gggtttttta
     2281 tttagatctt aactcgctga ataaactcac caaatgcggt caattgcccg gtcaggtggt
     2341 ccagcgtacc ctgatcaatc acttctccgg tttgcggatc aactttgttc tgaatcacgc
     2401 cgcccataaa ttccggcttg ttcatcacca ttgcatcgag gaaaaccaga atctggcgca
     2461 ggtgatactg acagcgcgcg ccgccaatca cgcccattga gctggtctga atcaacaccg
     2521 gtttacctgc cagcggttga tccggcaggc gggaaagcca gtcgatggca tttttcagcc
     2581 cacccggtac cgagtagtta tattccggcg tgacgatcac cacaccgtca gcctgacgga
     2641 tctgttccgc cagagcttca accgttgctg gaaaaccgtc ttcctgctgt acgtcagcgt
     2701 catacagagg aatgtcggca atggaaggta acgcattgac ttccatgctt gccggagcaa
     2761 ttttcggcag ggtacgtgca accatgccat taaatgagcc tttgcgcagg ctccccagta
     2821 acgtaaccac ctgcaatttt tcagacatga tgactcctgt ggttgtctat caatttggcg
     2881 aaaaagcctt cacggtagag agctgggcaa aacgcatcat ccggctggta ggattgttgg
     2941 cgcggctgtg aatatccggc aggcttttcc agccgtgttt gccatctaac tcccagacac
     3001 tgagcttttc aatcgtgggc gtgaccgcca cggaccacac cagcacgtct tccgcgtcgc
     3061 ataacgcttc tacatgattt acatgagcac atttcaggcg tccggcaatg ggcagcagct
     3121 gcagatcgcg cggatcgtca ttcaaaacat caccggttag ttttagcacg cttcggcgca
     3181 gcgtgatgag ctgcgcccgc gcttcgttag gatcgttttg tttactgatc catagcgatt
     3241 cattgcttga aaaggtgtgg ttatcgggcg cgtgtgggct atgaaacccg cgcgtcgcac
     3301 gctgtagttc catatcgagg ccacattcac cttcggttgt taacgccacg ccaaccatat
     3361 tcccggcata ggaaatagaa aacgaaggca aatttttatc gctgaaaacc ggtttacctt
     3421 tcggcagggt gacaatttcc ggcaattcgc caatgccata cagcatgaac atcagttctg
     3481 cgagtaaacc tctggacgcc agaaaacggc tttgacgatg aaggggaatg tttcgggctt
     3541 cttgatgaca ctgggagggg agacgaacag aaatcagatg tccttccgtt aaaatccctc
     3601 gggcaaagtg agtcgccatt tttcgctcca tgcttaatgg tccattatga atacaagagt
     3661 aatcatttac tggaatgatg gacagactaa agcggctaaa agttcgattc agacaagctt
     3721 attacaactt ttttacattt tattgtgaaa ttgggtcgcg tttcatacga ttcggttatg
     3781 ccatcgacgc gtaaaggttt ttaatggttt cccgtaacca gacgatcttc ggattatggc
     3841 tgttccgttt atgccacagc agggtaaaag gaacttccag ctttttttgc tggctttcgt
     3901 caaacgggag aggaagagca accaacggca gttgatggag ttgattgtag tactgacagt
     3961 agcgcggcgc ggtcgccagt agcagattgt cgggttgcgc tgccataaac agtgactgct
     4021 caaattccgg caggctcata gcaatcgtgc gttcgcgccc cagctcctgt aacacattgt
     4081 ccagcgccca ggtgtcgctc tgttcccagc aaatgctgat atgcggataa cgtaaaaagg
     4141 tgtccagatt ccacgtttca tgcagtgccg gatgatcttt gcgtaaccag acgcagggta
     4201 catcactaaa cagcacttca taatcaatgg ctaacggtag cgagcttaac agctcccgcg
     4261 agcgaggatg gctttcgcga ccggaaaaac caatatccac ttcaccacga gtaatggcat
     4321 ctaaggaatc gtaatcccag ttacgtagtt tgatggtcgc ctgcgggtaa cgttggtaga
     4381 tccgtttcga cagcgcatta agcatgatca tcatcagcgg cgattccgcc gccaactcaa
     4441 acttcaggcc gcgcggtgtc tggtggtgcg gtttatccag cagcagattg ctcatttgca
     4501 tccattccgc cagattttgc tccatgctga ccattagcgg tgtgggcgac aggcccagcg
     4561 gtgagttcac aaagagcggg tcgtcaaacc acgctcttaa ctttgccagc gacttactca
     4621 ccgctgaagg ggtcacgttc atccgcttcg ccgctttggt cacgctgcgc tcctgcatca
     4681 gcagttgcag acagagcagc agattgagat caagcgtggt gatggatttc ttcatgagcg
     4741 gcaacggggc gtccaggcgc gacgaacata atcagcaaca ggctcactat gctacaggca
     4801 atcagaatcc cgatcagcat attccatgcg ccgataccaa ccaccgctgc cagccaaatc
     4861 cacagtgacg aaccgcaaac ctgcgcaata cctaaggtcg agctggctac gcccgcgcgt
     4921 aatgaaaatg gtcctaacgc ctgactcatc gctacgccaa aacctaccga gaaaccggcg
     4981 caaatcagcg tgataccaaa cagagaaacc gcatgggaag gtgaaacggc aagggtgatc
     5041 cccgctgcaa ggaataacac ctgcgaggtg atcatcaacg tacgtggctt aaaaattccc
     5101 agcgcaaatg gcgtggagaa tgaaacggtc atgctgacgc cagcagtcaa cgccataatg
     5161 gtggcgtatt ctccgcgctc aaaacccatg atttccatca gcaataccgg cgatgtattg
     5221 acgaaagtga ggatcaccga aacgctgagg gtggtgataa caacacggct gaggaaaaaa
     5281 cggttaagca gcgactcgct attttctcgt ggtttgtccg aagctgcggg ggccgcaggg
     5341 cgcgtttctt ttaaaataaa caaagacaac atcagtaccg cgatgcccat cattgccatc
     5401 gcccagaaca gactctgcca cgggaattta agcataatca gatgtccgag cactggcgct
     5461 aacaccggaa tgatgcaggt aataccgttg agtaatgaca gcacttttgc ccgacgtcga
     5521 tcatccagcg tgtcgcgcaa aatggcgaac gccaccacgt aacaacagcc cgcgcccaac
     5581 ccctgtagaa agcgccctgc aagaaataac gtgctggttt cagccagtga acagaacacc
     5641 gaggcgataa taaatagcgc cgcgccgggg atggcgaccg gctttctccc tgaacgatcg
     5701 gccactttac cggcaaataa catcgcagct gccatcccag ccagataaac agagaacgca
     5761 atatgcaact gcgcttcgct ggcattgaga tcggcggcga tgcgcggtaa accaacgagg
     5821 tacatatcaa tcccggcggg ataaagtaaa accagggcaa aactacaaat caaaaagcgg
     5881 gacatgggct aaagcgtcct gatagtaagg tgacgcaaag catacgtggt gaaggagaat
     5941 caggcgagtt gccatttgga cataagtgat ttcctgacag gcaagaagcc agcgaaatgg
     6001 ctggcttcct gaaggattta gccaaattta ggtaacacgt taaagacgtt accgaaccag
     6061 cataaaatta cggttatacc aaagagaatg acaatcgccg gaataagatt accgccccag
     6121 accgtgaaca tctgattggg aaacttcttg cgagctttga ttgccagcac tgcgggaata
     6181 atgaccgccc agatggtggc gcacagtccg gcaccgccga tcccgtaaat aaagccgttc
     6241 gggaagatca gatacaacaa cgcgggtggc aggaaggtta acagcacggt tttgaaacgc
     6301 ccgccgtggg agttatcaat cttaaacaga tccgccagat aatcgaacaa tcccagcgtg
     6361 acaccaaaga acgaactggc aacagctaag ttggagaaca ccagcaggca aaactcgata
     6421 atcccatgct gtttggtgcc gaggaacgat ttcaccagcg aatcaacgtt gccgcctgag
     6481 gagattatcg ccttaaagct ttcgcgcgga atattcccca tcgtgcaata gagccagaag
     6541 agataaatca ccagcgccag cagcgaacca aataccacgc ttttgattaa cttatctttg
     6601 cgttttccat agcaaataat caggctggga atattgccgt ggaaaccaaa tgacgccaga
     6661 cacaccggca aagccataaa gatatacggg aagtaagacg ttcccgcagt ggtgctggtg
     6721 gcatcgcgca ggatggagta atcgacctgg aagaagaaag aaccaaacac gatcacaaag
     6781 gagataatct tcagcccgag gaacaatgag gtaatgcgac tggcggctaa cgaacttatc
     6841 cacaatacgc tggcaacgaa aatggctgtg cagatcccga caatacgtgg attagcgtga
     6901 tagcccaaat tcattgatat cgtttcacta atgatcgcac cattagcaga gatataggca
     6961 taagtgagga tatagagaac gaaggcaacg gtaataccgc tgataatgtt ccaggtgtta
     7021 ccgattaaat ctttggtgat ggtgttaaaa ctggagccga caggataatt taaatttgct
     7081 tctaataaca ataacccgga atgaagcatt gaaaaccagg caatgataag gataaaggca
     7141 ccccagaaaa accaggcacc ggcaagatca acaggtaaag caaacatacc tccaccaatt
     7201 actgtacctg ctataaccat aacaccccaa aatgcagagt gctttttttc agcttgatca
     7261 gtcatgatgc cacctttaga ggaaggctat ttttgttatt gaggatgtag ggtaagagag
     7321 tggctaacat ccttatagcc actctgtagt attaattaaa cttctttcag ttttgcggtg
     7381 aagtgacgca atacttttgg ttcgtaggta aaggttaatc ctttaatatt cgccgcgttc
     7441 tctttcacat gtttaaaggc ttcaataatg aagtccatat gtgtttgagt atatgttgcg
     7501 cgcggaatgg ttaaacgcag cagttcagcc gggcatggca gttgtttacc ggttttcgga
     7561 tcgcggccta acaggaaaga gccaatttct accgcacgga taccggcgac tttatacagc
     7621 tcgcacgcca gcgcctgtgc cgggaactgg tctgccggga tatgcggcag cagtttaccg
     7681 gcatcaacga atgccgcgtg accgcccgcc tgctggcaga caacgccaat ctcttccaga
     7741 ccatcgacca gatactgtac ctgcgcgata cgataagcca gccagtcgag attcatgccg
     7801 tcatacagac ctaccgccag acgctccatc gcgccgcctt ccaggccgcc atatgtcggg
     7861 aacccttcct gcaccacgca aagggttctg cactcggtgt acacatcaaa gaagctgtcg
     7921 tctttcatgc acagcaagcc gcccatcggt accatcgcat ctttcttggc ggacatcgcc
     7981 agcatatcgg catatttgta ggtttcgcgg gtgatctgct cgatggtcca gtctttgtat
     8041 tctgcttcac gctgcttgat gaaataggcg ttttcagcaa agcgtgcgga gtccattacc
     8101 accggaatat cgtatttctt cgcgatgctg tacatcgctt ttaagtttgc cagtgaaacc
     8161 ggctgaccac ctgcagagtt actggtgatg gttgcaacga tatacggcac gttattcggg
     8221 ccaacttctt caataccgcg ttctaatcct tcgaggtcaa agttgccttt aaagtcgtaa
     8281 cgcacgcccg tatcgaaggc ttctttgata tagacgttac gcacggtaca gccgttaatc
     8341 tggctatggc cctgcgtggt atcaaagaaa tagttagaga atgccaccat tttgctgcga
     8401 tccaggcctt tttcctgctc gcgtttttta atcagtaccg gaatatagat ttgctctgcg
     8461 ccacggccct ggtgagtcgg aatagtatat tgataaccaa agatattttt cactgactcg
     8521 gctaacgcat agtagctgcg gctgccgctg taggcttcgt cgccgcgcat catcgctgcc
     8581 tgcatacttt gggttaccgc cccggtgccg ctgtcggtca gtaaatcgat aaacacatct
     8641 tcgctatcca gcaggaacgg gttcataccg gatttaataa ttgcttcttc acgataagcg
     8701 cgagtagtac gttttactgg ctcaataaca cgaatgcgga acggttcagg gagatgttta
     8761 aagttttcca ttacataatc cttcatttat tttaattaca gtgatccctg tgaatattac
     8821 atctgcaata tccattaatt cgccatgtat atatacaagc accatcattt actaaaataa
     8881 gtaaataaac gagagatggc ctttgcaaaa ggcaaaatta agatatagca aactaatcgt
     8941 tcacattgaa tctaatcggt ttggctctgg tgatggctac agaagggtaa attaagggcg
     9001 gtgatcgaca atcttattgt cgatattgaa ccattttgag gtcacacata tatgtaagat
     9061 attcataatg cacttatcct cgaaagacac gaggctacag tataatactc aagaattttg
     9121 tctattctaa aattgtttaa atgtgaatcg aatcacaatc gttcggggag caatactaaa
     9181 tgcttttttt taagattata aattacgtaa gaattttaat gtttaaaaaa agggttgagg
     9241 aataacagga gtgctaacaa tgaagcgaag ccgcatctga cagtcagaat acggcttcgt
     9301 aagcgcggtt acttaccaat acagaagctg gagaaaatcc gccccagcag gtcgtctgaa
     9361 gtaaattccc cggtgatttc gcttaagttc tgctgtgcca ggcgcaactc ttccgccagc
     9421 agttcacccg cccaggcacc taacagttgc gctttacctt gctgcaaatg ctccgctgcc
     9481 tgttccagcg cctgtaggtg gcgacgacgc gccaggaagc cgccttccat gttggtgtca
     9541 aagcccatgc tctgtttgag atggttacgc agcacctcca cgccttcacc tgtccttgcc
     9601 gagagacgaa ttaacgcgtg accgttcact tcactcattc ccagcgtttc gccggtgata
     9661 tcggctttat tgcgcaccac ggtgatcggc agtttcgctg gcagacgggc gataaattcc
     9721 ggccagatct ctgccggatc cacggcgtct gttgtggtgc catcgaccat aaacagcacg
     9781 cggtcggcct gttcaatttc ctgccacgcg cgctcgatac caatacgttc cacttcgtca
     9841 ctggcttcac gtagcccagc ggtatcgatg atatgcagcg gcattccgtc aatgtggata
     9901 tgctcacgca gcacgtcacg tgtggttccg gcgatatcag ttacgattgc tgcttcacgg
     9961 cccgccagcg cgtttaacag gctcgattta ccggcgttag gacgtccggc aatcaccacc
    10021 ttcatccctt cgcgcaacaa actaccctga cgtgcttcag cacgcactgc atcgagatcg
    10081 gcaatcacgt cattaagctg ggcttcaatt ttgccgtcgg agaggaaatc gatctcttca
    10141 tcagggaaat caattgccgc ttcgacgtaa atgcgcaagt gggtgagggc ttccaccaga
    10201 tggttgaccc gtgcggagaa tgcgccttgc agcgagttaa gtgccgaacg ggccgcctgt
    10261 tccgaactgg cgtcgataag atcggcaatc gcctcggcct gggctaagtc gagtttatcg
    10321 ttaagaaacg cgcgttcgga aaactcacca gggcgagcaa tccgcaggcc gggaatggtc
    10381 agaatgcgtt ttaacagcag gtcgaggatc accggaccgc catgaccttg cagttccagc
    10441 acatcttcgc cggtgaacga gttcgggcca gggaaccata gcgcaatccc ctgatcgagc
    10501 acgctgccgt cggcgtcttt aaacggaaga taatcggcgt agcgcggctt aggtagttta
    10561 cccagcacgg tttcggcaac ttcacgggct ttgaagccgg agatgcgcag gatgccaacg
    10621 ccgccacgtc ccggaggcgt ggcctgggct acgatagtgt cattatcgct catgatgttc
    10681 ctgttgcttt gtgtggcgga tgcgcggtgc ttatccgccc tacgaaaaga aaaaaggcgg
    10741 tcaactgacc gcccttattt tagcgaaaac tcaccgaatc aggatttttt cttctcgcgg
    10801 ctatgcaggc cacgtttttc cagaccacgg taaatcagct gctgctgaat aatggttacc
    10861 aggttgctga cgatatagta cagcaccaga cctgacggga accacaggaa gaacacggtg
    10921 aagatgaccg gcataaaggt catgatcttc tgctgcatcg ggtcggtcac tgtggtcggc
    10981 gacatcttct gaatgaagaa catcgttacg cccatcagga tcggcaggat gtagtacggg
    11041 tcctgtgccg acaggtcgtg gatccacagt gcaaacggtg cctgacgcag ttcaacggaa
    11101 cccatcagca tgtagtacaa cgccaggaag attggcatct ggatcagcag cgggaagcag
    11161 ccgcccagcg ggttaacctt ctcagctttg tacagcgcca tcatttcctg gctgatacgc
    11221 tgtttgtcat cacccagacg ctcacgcatt gcctgaatct tcggctgcag catacgcatc
    11281 ttcgccatgg aggtgtactg cgctttggtc agcgggtaca tgatgccacg aacgataaag
    11341 gtgatgatga taatggagaa gccccagtta cccacaaagc tatggatcca tttcagcagt
    11401 ttgaacagcg gctgagagat gaaccacaac caaccgtaat caacggtcag atccaggtgc
    11461 ggagcaacag ctgccatttt gtcctggatt tccgggccaa cccacagggt gctgttcatc
    11521 gcgccagtct gaccaggctg aaccagtacc ggctgagatt tatagccgat agcggcgatg
    11581 ccgttaccca gattagcggt atagaagttg ttggtaccgt cgttatgcgg gatccacgcc
    11641 gtcgcgaaat actgttgcag catcgccacc caaccacctt tcgaagagat gttcaggttt
    11701 tcgttatcgg caatggtatc gaacttgtat ttctcatact tctcgtcagg cgtggagtac
    11761 gccgcgccac ggaaggtgtg cagtgcgaag ttgctgcttc cggtatcgag atgcggtggc
    11821 agagtgatgg attgcttcaa ctgaccaaag gtggagattt ccagcggttt ctcgccagcg
    11881 ttctgcacgt tgtagttgac gttgacagcg taatcaccac gtttcaggac aaacgttttg
    11941 gtaaacgtgt tgcctgccgc gtcggtatac gtcatcggca cctgcagttc gttttgacct
    12001 tcagccagca cataagcgtc tttttcaacg ttatacagcg gacgcgggcc gttagccggg
    12061 ttatccgggc catcacgacc ggtcagaccg ctctgtgcct gataaataaa ctgcggtgaa
    12121 gtttccagca gctggaacgg ctgggtagag ttcagctctt tcgggtaagc cggcagcaga
    12181 gcttgctcaa catcaccacc acgggtgttg atggtcagat caagcacgtc ggtcttaacc
    12241 gagatcagtt tcccctggcc actggccggt acgccctggt cggcggcgct acccgctgcg
    12301 gtggtcgttg tctgcgtggt ctgttgggcc tgaggttgcg ggtttttatc ctgctcccag
    12361 gcttgccaga tcatgaaaga cacgaacagc aaagcgatga ctaaaagatt gcgttgcgaa
    12421 tccatcgtta gtgttctctg gtatcaaatg gtccgggcgg gacgggatcg tcaccaccag
    12481 ggtgtaaagg gtggcatttt aatacgcgtt tcaccgtcaa ccaactgcct tttatcactc
    12541 caaacctgcg caatgcctca attccgtagc ttgaacaggt tggagtgaaa cgacaatgcg
    12601 gcccgagtag cggactaatc aggcgttgat agacccgaat gagggctatc aggacccgcg
    12661 agccaggcga cagtggcggc gccataattt ttccaacgct tccgagagag cacggttatc
    12721 gaggtcggca accccttttt tcgccaccac cacgaaatcc atagccggga gttcatgttg
    12781 gcgcagacgg aagctttcac gcgtcagacg tttaatccga ttgcgttcat gggcgcgtcg
    12841 aacgtttttc ttggcgactg taagaccgat acggggatgc cccagcgaat tcaggcggcc
    12901 gagaatggta atttgcggcg tgccagcccg ttgtggctgc tggaagacga atgtgaattg
    12961 actgggagtt aacaagcgta actccctggg aaatgcgagc ttaaccactc aggggttagc
    13021 tttattactt agaaacggtc agacgagcgc ggcctttagc acgacgacgt gccagaacct
    13081 gacgaccatt tttagtagcc atacgagcac ggaagccgtg agaacggttg cgcttcagta
    13141 cagacggttg aaaagtgcgt ttcatggcga tttctaccta aacttgaata aattcaatgg
    13201 ctttattgga tatccgccga aaaatgaaac gatggacacc gaagccatgg gtgattaaag
    13261 aggccggatt gtaataattg tacactccgg agtcaattct ctttccttat ttaccgcgct
    13321 tttccgcacc ttttcgcagg gaaaatgtac gacctcacac cagtggaaac cagcatggcg
    13381 cgccgggtgg aggattatac gggctgatgg gtaaagcgca aggatcgtcc tggatcttta
    13441 ttagatcgat taagccaatt tttgtctatg gtcattaaat tttccaatat gcggcgtaaa
    13501 tcgtgcccgc ctcgcggcag gatcgtttac acttagcgag ttctggaaag tcctgtggat
    13561 aaatcgggaa aatctgtgag aaacagaaga tctcttgcgc agtttaggct atgatccgcg
    13621 gtcccgatcg ttttgcagga tcttgatcgg gcatataacc gcagacagcg gttcgtgcgt
    13681 caccctcaag cagggtcttt tcgacgtacg tcaacaatca tgaatgtttc agccttagtc
    13741 attatcgact tttgttcgag tggagtccgc cgtgtcactt tcgctttggc agcagtgtct
    13801 tgcccgattg caggatgagt taccagccac agaattcagt atgtggatac gcccattgca
    13861 ggcggaactg agcgataaca cgctggccct gtacgcgcca aaccgttttg tcctcgattg
    13921 ggtacgggac aagtacctta ataatatcaa tggactgcta accagtttct gcggagcgga
    13981 tgccccacag ctgcgttttg aagtcggcac caaaccggtg acgcaaacgc cacaagcggc
    14041 agtgacgagc aacgtcgcgg cccctgcaca ggtggcgcaa acgcagccgc aacgtgctgc
    14101 gccttctacg cgctcaggtt gggataacgt cccggccccg gcagaaccaa cctatcgttc
    14161 taacgtaaac gtcaaacaca cgtttgataa cttcgttgaa ggtaaatcta accaactggc
    14221 gcgcgcggcg gctcgccagg tggcggataa ccctggcggt gcctataacc cgttgttcct
    14281 ttatggcggc acgggtctgg gtaaaactca cctgctgcat gcggtgggta acggcattat
    14341 ggcgcgcaag ccgaatgcca aagtggttta tatgcactcc gagcgctttg ttcaggacat
    14401 ggttaaagcc ctgcaaaaca acgcgatcga agagtttaaa cgctactacc gttccgtaga
    14461 tgcactgctg atcgacgata ttcagttttt tgctaataaa gaacgatctc aggaagagtt
    14521 tttccatacc ttcaacgccc tgctggaagg taatcaacag atcattctca cctcggatcg
    14581 ctatccgaaa gagatcaacg gcgttgagga tcgtttgaaa tcccgcttcg gttggggact
    14641 gactgtggca atcgaaccgc cagagctgga aacccgtgtg gcgatcctga tgaaaaaggc
    14701 cgacgaaaac gacattcgtt tgccgggcga agtggcgttc tttatcgcca agcgtctacg
    14761 atctaacgta cgtgagctgg aaggggcgct gaaccgcgtc attgccaatg ccaactttac
    14821 cggacgggcg atcaccatcg acttcgtgcg tgaggcgctg cgcgacttgc tggcattgca
    14881 ggaaaaactg gtcactatcg acaatattca gaagacggtg gcggagtact acaagatcaa
    14941 agtcgcggat ctcctttcca agcgtcgatc ccgctcggtg gcgcgtccgc gccagatggc
    15001 gatggcgctg gcgaaagagc tgactaacca cagtctaccg gagattggcg atgcgtttgg
    15061 tggtcgtgac cacacgacgg tgcttcatgc ctgccggaag atcgagcagt tgcgtgaaga
    15121 gagccacgat atcaaagaag atttttcaaa tttaatcaga acattgtcat cgtaaaccta
    15181 tgaaatttac cgtagaacgt gagcatttat taaaaccgct acaacaggtg agcggtccgt
    15241 taggtggtcg tcctacgcta ccgattctcg gtaatctgct gttacaggtt gctgacggta
    15301 cgttgtcgct gaccggtact gatctcgaga tggaaatggt ggcacgcgtt gcgctggttc
    15361 agccgcacga acctggtgcg acgaccgttc cggcgcgcaa attctttgat atctgccgtg
    15421 gtctgcctga aggcgcggaa attgccgtgc agctggaagg tgaacggatg ctggtacgct
    15481 ccgggcgtag ccgtttttcg ctgtctaccc tgccagcggc ggatttcccg aacctcgatg
    15541 actggcagag tgaagtcgaa tttaccctgc cgcaggcaac gatgaagcgt ctgattgaag
    15601 cgacccagtt ttctatggcg catcaggacg ttcgctatta cttaaatggt atgctgtttg
    15661 aaaccgaagg tgaagaactg cgcaccgtgg caaccgacgg ccaccgtctg gcggtctgtt
    15721 caatgccaat tggtcaatct ttgccaagcc attcggtgat cgtaccgcgt aaaggcgtga
    15781 ttgaactgat gcgtatgctc gacggcggcg acaatccgct gcgcgtacag attggcagca
    15841 acaacattcg cgcccacgtt ggcgacttta tcttcacctc caaactggtg gatggtcgct
    15901 tcccggatta tcgccgcgtt ctgccgaaga acccggacaa acatctggaa gctggctgcg
    15961 atctgctcaa gcaggcgttt gctcgcgcgg cgattctctc taacgagaaa ttccgcggcg
    16021 tacgtcttta tgtcagcgaa aaccagctga aaatcaccgc caacaacccg gaacaggaag
    16081 aagcggaaga gatcctcgac gttacctata gcggtgcgga gatggaaatc ggcttcaacg
    16141 tcagctatgt gctggatgtt ctgaacgcgc tgaaatgcga aaacgtccgc atgatgctga
    16201 ccgattcggt ttccagcgtg cagattgaag atgccgcatc acagtcggct gcctatgttg
    16261 tcatgccaat gagactgtaa tgtccctcac ccgcttgttg atccgcgatt tccgcaacat
    16321 tgaaaccgcg gatctcgctt tatctcccgg ctttaacttt ctggtaggtg ccaacggcag
    16381 tggcaaaacc agcgtgctgg aagccatcta tacgctcggc catggtcggg cgtttcgcag
    16441 tttgcagatt ggtcgcgtca ttcgccatga gcaggaggcg tttgttctcc acgggcgatt
    16501 acagggcgaa gagcgcgaga cagcgattgg cttaaccaaa gacaaacagg gcgacagcaa
    16561 agtccgcatc gacggtacag acgggcataa agtcgcggaa ctggcgcacc tgatgccaat
    16621 gcagttgata acgccagaag ggtttacttt actcaacggc ggccccaaat acagaagagc
    16681 attcctcgac tggggatgct ttcacaacga acccggattt ttcaccgcct ggagcaatct
    16741 caagcgattg ctcaagcagc gcaatgcggc gctgcgccag gtgacacgtt acgaacagct
    16801 acgcccgtgg gataaagaac tgatcccgct ggcggagcaa atcagcacct ggcgcgcgga
    16861 gtatagcgcc ggtatcgcgg ccgatatggt cgatacctgt aagcaatttc tccctgagtt
    16921 ttctctgact ttctctttcc agcgcggctg ggagaaagag acagaatatg ctgaggtgct
    16981 ggaacgtaat tttgaacgcg atcgccagct aacctacacc gcgcatggcc cgcataaagc
    17041 ggacttacgc attcgcgccg acggtgcgcc ggtggaagat accttatcgc gtgggcagct
    17101 taagctgttg atgtgcgcct tacgtctggc gcaaggagag ttcctcaccc gtgaaagcgg
    17161 gcggcggtgt ctctacctga tagatgattt tgcctctgag cttgatgatg agcgtcgcgg
    17221 gctgcttgcc agccgcttaa aagcgacgca atcacaggtc tttgtcagcg cgatcagtgc
    17281 tgaacacgtt atagacatgt cggacgaaaa ttcgaagatg tttaccgtgg aaaagggtaa
    17341 aataacggat taacccaagt ataaatgagc gagaaacgtt gatgtcgaat tcttatgact
    17401 cctccagtat caaagtcctg aaagggctgg atgcggtgcg taagcgcccg ggtatgtata
    17461 tcggcgacac ggatgacggc accggtctgc accacatggt attcgaggtg gtagataacg
    17521 ctatcgacga agcgctcgcg ggtcactgta aagaaattat cgtcaccatt cacgccgata
    17581 actctgtctc tgtacaggat gacgggcgcg gcattccgac cggtattcac ccggaagagg
    17641 gcgtatcggc ggcggaagtg atcatgaccg ttctgcacgc aggcggtaaa tttgacgata
    17701 actcctataa agtgtccggc ggtctgcacg gcgttggtgt ttcggtagta aacgccctgt
    17761 cgcaaaaact ggagctggtt atccagcgcg agggtaaaat tcaccgtcag atctacgaac
    17821 acggtgtacc gcaggccccg ctggcggtta ccggcgagac tgaaaaaacc ggcaccatgg
    17881 tgcgtttctg gcccagcctc gaaaccttca ccaatgtgac cgagttcgaa tatgaaattc
    17941 tggcgaaacg tctgcgtgag ttgtcgttcc tcaactccgg cgtttccatt cgtctgcgcg
    18001 acaagcgcga cggcaaagaa gaccacttcc actatgaagg cggcatcaag gcgttcgttg
    18061 aatatctgaa caagaacaaa acgccgatcc acccgaatat cttctacttc tccactgaaa
    18121 aagacggtat tggcgtcgaa gtggcgttgc agtggaacga tggcttccag gaaaacatct
    18181 actgctttac caacaacatt ccgcagcgtg acggcggtac tcacctggca ggcttccgtg
    18241 cggcgatgac ccgtaccctg aacgcctaca tggacaaaga aggctacagc aaaaaagcca
    18301 aagttagcgc caccggtgac gatgcgcgtg aaggcctgat tgcggtcgtt tccgtgaaag
    18361 tgccggaccc gaaattctcc tcccagacca aagacaaact ggtttcttct gaggtgaaat
    18421 cagcggttga acagcagatg aacgaactgc tggcagaata cctgctggaa aacccaaccg
    18481 acgcgaaaat cgtggttggc aaaatcatcg atgctgcccg tgcccgtgaa gctgcgcgtc
    18541 gtgcgcgtga aatgacccgc cgtaaaggtg cgctcgactt agcgggcctg ccgggcaaac
    18601 tggcagactg ccaggaacgc gatccggcgc tttccgaact gtacctggtg gaaggggact
    18661 ccgcaggcgg ctctgcgaag caggggcgta accgcaagaa ccaggcgatt ctgccgctga
    18721 agggtaaaat cctcaacgtc gagaaagcgc gcttcgataa gatgctctct tctcaggaag
    18781 tggcgacgct tatcaccgcg cttggctgtg gtatcggtcg tgacgagtac aacccggaca
    18841 aactgcgtta tcacagcatc atcatcatga ccgatgcgga cgtcgacggc tcgcacattc
    18901 gtacgctgct gttgaccttc ttctatcgtc agatgccgga aatcgttgaa cgcggtcacg
    18961 tctacatcgc tcagccgccg ctgtacaaag tgaagaaagg caagcaggaa cagtacatta
    19021 aagacgacga agcgatggat cagtaccaga tctctatcgc gctggatggc gcaacgctgc
    19081 acaccaacgc cagcgcaccg gcattggctg gcgaagcgtt agagaaattg gtgtctgagt
    19141 acaacgcgac gcagaaaatg atcaaccgca tggagcgtcg ttatccgaaa gcaatgctga
    19201 aagagcttat ctatcagccg acgctgacgg aagccgacct ctctgatgag cagaccgtta
    19261 cccgctgggt gaacgcgctg gtcagcgaac tgaacgacaa agaacagcac ggcagccagt
    19321 ggaagtttga tgtccgcacc aatgccgaac aaaacctgtt tgaaccgatt gttcgcgtgc
    19381 gtacccacgg tgtggatact gactatccgc tggatcacga gtttatcacc ggtggcgaat
    19441 atcgtcgtat ctgcacgctg ggtgagaaac tgcgtggctt gctggaagaa gatgcgttta
    19501 tcgaacgtgg cgagcgtcgt cagccggtag ccagcttcga gcaggcgctg gactggctgg
    19561 tgaaagagtc ccgtcgcggc ctctccatcc agcgttataa aggtctgggc gagatgaacc
    19621 cggaacagct gtgggaaacc accatggacc cggaaagccg tcgtatgctg cgcgttaccg
    19681 ttaaagatgc gattgctgcc gaccagttgt tcaccacgct gatgggcgac gccgttgaac
    19741 cgcgccgtgc gtttattgaa gagaacgccc tgaaagcggc gaatatcgat atttaatggc
    19801 gctaaccatg cgagcgtgcc tgatgcgcta cgcttatcag gcctacgaaa atcttgcaat
    19861 gtattgaatt ttcatgattt tgtaggccgg ataaggcgtt cacgccgcat ccggcaagaa
    19921 gctataagaa aagggcggag atcatctccg ccctttttat ttctgcaatc cgcaattatc
    19981 atcccgcaaa tctatacttt ttgggtcaac acgtaacgct ttacaggtga ttatcatggg
    20041 gctttttgat gaagttgtcg gtgcctttct gaaaggcgat gcggggaaat atcaggctat
    20101 tttaagttgg gttgaggagc agggcggcat tcaggtgctg ctggaaaaac tgcaaagtgg
    20161 cggcttaggg gccattctct caacctggct gagtaatcaa cagggcaatc aatcggttag
    20221 tggcgagcaa ctggaatcgg cgctcggcac aaatgcggtg tccgatctcg ggcaaaaact
    20281 tggcgtggat accagtacag cttccagttt actggcagag caattgccga agattattga
    20341 tgcgctctca ccgcaaggtg aagtgtcacc acaagccaat aacgatctgc tttccgcagg
    20401 tatggaactg ctgaaaggga aactcttccg ctaagcaaat ggggagcatg caacccgcgt
    20461 tgcatccccc ttttccctcg ggtgttgtac tgatttttga gcggaatcgc gttagcatgg
    20521 gtcaggaacc aatctacctg gggaactcat ggctatcaaa ctcattgcta tcgatatgga
    20581 tggcaccctt ctgctgcccg atcacaccat ttcacccgcc gttaaaaatg cgattgccgc
    20641 agctcgcgcc cgtggcgtga atgtcgtgct aacgacgggt cgcccttatg caggcgttca
    20701 caactacctg aaagagctgc atatggaaca gccgggcgac tactgcatta cttataacgg
    20761 cgcgttggta cagaaggccg ccgatggtag caccgtggcg caaaccgctc tcagctatga
    20821 cgactaccgt ttcctggaaa aactctctcg cgaagtcggt tctcatttcc acgccctgga
    20881 ccgcaccacg ctgtatacag caaatcgcga tatcagctac tacacggtgc atgaatcctt
    20941 cgttgccacc attccgctgg tgttctgcga agcggagaaa atggacccca atacccagtt
    21001 cctgaaagtg atgatgattg atgaacccgc catcctcgac caggctattg cgcgtattcc
    21061 gcaggaagtg aaagagaaat ataccgtgct gaaaagtgcg ccgtacttcc tcgaaatcct
    21121 cgataaacgc gttaacaagg gtacgggtgt gaaatcactg gccgacgtgt taggtattaa
    21181 accggaagaa atcatggcga ttggcgatca ggaaaacgac atcgcaatga ttgaatatgc
    21241 aggcgtcggt gtggcgatgg ataacgctat tccttcggtg aaagaagtgg cgaactttgt
    21301 caccaaatcc aaccttgaag atggcgtggc gtttgctatt gagaagtatg tgctgaatta
    21361 atcagtggac gggcaaacag cataaacgcg tttgcccgct tactgattat tggtttactg
    21421 catatacaac cagaaattgc agcggtgcag taatgatgtc cagcgtaaca gtcactggca
    21481 gtaatgctgc ggaaacacca tccggaatgc ctctgggtga atagtattca taaaatcgca
    21541 ctttaaatgg atggtagaac gccatcacct ttgagtaatc catgttttta tttttctcat
    21601 ggattgtgcc ttttaggttt tccagtgacc tggtacagtg ttgaacacca caggcaaaac
    21661 cataatgcgt tgccagtgcc ttttcttctt cgttattcca gtagtagtaa agcgatattg
    21721 tgccggtaaa tttctttttc cccgcattta aaacaaatct tgcgtcatcg gcaacctgaa
    21781 tattgtgccg gttaagtgct ggatcattga gcatcttaac gatgtcatcg gcaccatcag
    21841 tgataaggta atccagcgat tcccctacaa aaacatagcc ttttgttcca ttactatctt
    21901 ttgcctgcga tagcccagtg atggtgtctg actgcgattg atactttcct gaactaccct
    21961 ttgcggtatg ggaaaccaga gcatacgaga tacagcctga aagcatcagc gccagtggga
    22021 ataaaccggc agccttaaaa aatcccttaa aattcaactt catgattaat cctttaaacg
    22081 agtagctaag ccgttgaaag catactcttc tttccctcct gtttagcgcc tgatatccct
    22141 tttcagattt ctgcccgacg catgtcattt ttttatgcat tgttcttttt gtgatctaaa
    22201 ttgtagtaca acaatataag tttgtactac attacgcaca cggcaaacgc gaacgtcatc
    22261 acgctggtac tacaaagttg ccgcgttatg catcgatcgg ggtaaagtag agaagaacat
    22321 acagagcaca aggactctcc atgactctca ataaaaccga tcgcattgtc attacgctgg
    22381 gtaaacagat cgttcacggc aaatacgtac ctggctcgcc acttccggct gaggcggagc
    22441 tctgtgaaga gtttgcaacc tcgcgcaaca tcatccgtga ggtgttccgt tcgctgatgg
    22501 cgaagcggct gattgaaatg aaacgttatc gcggggcgtt tgtggcaccg cgtaaccagt
    22561 ggaattacct cgacactgac gtactgcaat gggtgttgga aaacgactac gacccacggc
    22621 ttatcagtgc catgagcgaa gtgcgaaatc tggtggaacc ggcgattgcc cgttgggcag
    22681 cagagcgcgc gacttccagc gatctggcgc agattgaatc ggcgctgaac gagatgattg
    22741 ccaacaatca ggaccgcgaa gcgtttaacg aagcggatat tcgctaccac gaggcggtgc
    22801 tgcagtcggt acataacccg gtgttacagc aacttagcat tgcgatcagt tcgttgcagc
    22861 gggcggtttt tgaacgaacc tggatgggcg atgaggccaa catgccgcaa acgctccagg
    22921 aacataaggc gctgttcgat gcaatacggc atcaggacgg cgatgcggca gagcaggcgg
    22981 cgcttaccat gatcgccagc tcgacacgaa ggttaaagga aatcacatga cagctcgcta
    23041 catcgcaatt gactggggat cgaccaatct gcgcgcctgg ctttatcagg gcgaccactg
    23101 cctggagagc aggcaatcag aagcaggcgt cacgcgcctg aacggaaaat ctccggctgc
    23161 ggtgttagca gaagtcacga ccgactggcg tgaagaaaat acgccagtgg tgatggcagg
    23221 aatggtcggc agcaatgtcg gctggaaagt tgctccgtat ttatctgttc ctgcccgttt
    23281 ttcgtctatt ggcgaacaat taacgtctgt tggcgacaat atctggatta ttcccggatt
    23341 atgcgtctct catgacgata accacaatgt gatgcgcggc gaagaaacac aattgatcgg
    23401 cgcgcgagct ctggctcctt cctctcttta tgttatgccc ggtacgcatt gcaaatgggt
    23461 gcaggccgat agccagcaaa tcaacgattt tcgcaccgtg atgaccggtg aattacatca
    23521 tttactgtta aatcactcat tgattggcgc aggtttgccg ccgcaggaaa actctgccga
    23581 tgccttcgcg gctggccttg agcgcggcct taatgcgccc gccatattgc cgcagctttt
    23641 tgaagttcgc gcctcgcatg tgctgggaac acttccctgc gaacaggtca gcgaatttct
    23701 ctctggtttg ttgattggcg cagaggttgc cagtatgcgc gactatgtga cccatcaaca
    23761 cgccatcacc cttgtcgccg gaacatcgct gaccgcgcgc taccagcaag cctttcaggc
    23821 gatgggttgc gacgtgacgg cggtggcggg cgacacggca tttcaggctg gtataaggag
    23881 catcgctcat gcagtggcaa actaaacttc cgctgatcgc cattttgcgc ggcattaagc
    23941 ccgacgaggc gctggcgcat gttggcgcgg tgattgacgc cgggttcgac gcggttgaaa
    24001 tcccgctgaa ttccccacaa tgggagcaaa gcattcccgc catcgttgat gcgtatggcg
    24061 acaaggcgtt gattggcgca ggtacggtac tgaaacctga acaggtcgat gcgctcgcca
    24121 ggatgggctg tcagctcatc gttacgccca atatccatag tgaagtgatc cgccgtgcgg
    24181 tgggctacgg catgaccgtc tgccccggct gcgcgacggc gaccgaagcc tttaccgcgc
    24241 tcgaagcggg cgcgcaggcg ctgaaaatat ttccgtcatc ggcttttggt ccgcaataca
    24301 tcaaagcgtt aaaagcggta ttgccatcgg acatcgcagt ctttgccgtt ggcggcgtga
    24361 cgccagaaaa cctggcgcag tggatagacg caggttgtgc tggggcgggc ttaggcagcg
    24421 atctctatcg cgccgggcag tccgtagaac gcaccgcgca gcaggcagca gcatttgtta
    24481 aggcgtatcg agaggcagtg caatgaaaat caccaaaatt accacgtatc gtttacctcc
    24541 ccgctggatg ttcctgaaaa ttgaaaccga tgaaggcgtg gtcggttggg gcgagcccgt
    24601 gattgaaggc cgcgcccgta cggtggaagc ggcagttcac gagctgggtg actatttgat
    24661 tggtcaggat ccttcgcgca tcaatgactt atggcaagtg atgtatcgcg ccggatttta
    24721 tcgtggcggt ccaatcctga tgagcgccat tgccgggatc gaccaggcgt tatgggatat
    24781 caaaggcaaa gtgctgaatg cgccggtctg gcaactgatg ggcggcctgg ttcgcgacaa
    24841 aattaaagcc tacagttggg tcggcggcga tcgtccggcg gatgttatcg acggcattaa
    24901 aaccctgcgc gaaatcggct tcgatacctt caaactgaac ggttgtgaag aactggggct
    24961 aattgataac tcccgcgcgg tagatgcggc agtcaacacc gtggcacaaa ttcgtgaagc
    25021 ttttggcaat cagattgagt ttggtcttga tttccacggt cgcgtcagcg cgccaatggc
    25081 gaaagtgctg attaaagaac tggagccgta tcgcccgctg tttattgaag agccggtgct
    25141 ggcggaacaa gccgaatact acccgaaatt ggcggcacaa acgcatattc cactggcggc
    25201 gggtgagcgc atgttctcac gtttcgattt taaacgcgtg ctggaggcag gcggtatttc
    25261 gattctgcaa ccggatctct cccatgcagg cggtattacc gaatgctaca aaattgctgg
    25321 aatggcagaa gcctatgatg tgacccttgc gccgcactgt ccgctcggac cgattgcact
    25381 ggcggcttgc ctgcatatcg actttgtttc ctataacgcg gtacttcagg aacaaagtat
    25441 gggcattcat tacaacaaag gcgcggagtt actcgacttt gtgaaaaaca aagaagactt
    25501 cagtatggtt ggcggcttct ttaaaccgtt aacgaaaccg ggcttaggtg tggaaatcga
    25561 cgaagctaaa gttattgagt tcagtaaaaa tgccccggac tggcgtaatc cgctctggcg
    25621 tcatgaagat aacagcgtag cagagtggta attcctgcca cgtaagcccc tcatcgggca
    25681 ctaaaacagc aatacaaaaa tataaccctc tgtaaattac agggcatggt gagcggcttc
    25741 gctatgccca aaatctggag aaagctcgct atggatattc ccgttaatgc agcaaagccg
    25801 gggcgtcggc gttatctgac gctggtgatg atctttatta cggtggtcat ttgttatgtt
    25861 gaccgcgcta acctggccgt ggcttccgcc catattcagg aagagttcgg cattacgaaa
    25921 gcggaaatgg gctatgtatt ttcggccttc gcctggcttt atacgctatg ccagatcccc
    25981 ggcggttggt ttttagatcg cgtaggttct cgcgtgactt attttattgc gatatttggt
    26041 tggtcagtgg cgactttatt ccagggcttt gccacgggat taatgtcatt aattggtctg
    26101 cgtgcgataa ccggtatttt cgaagcgccc gctttcccga ccaataaccg gatggtgacc
    26161 agctggttcc cggaacatga acgcgcttcc gccgttggtt tttatacgtc tggtcagttt
    26221 gtcggtctgg cgtttttgac tccgctgctg atctggattc aggagatgtt gagctggcac
    26281 tgggtgttca ttgtcactgg tggtatcggc attatctggt cgctgatttg gtttaaggtt
    26341 tatcagccgc cgcgcctgac caaaggtatc agcaaagctg aactggatta cattcgtgat
    26401 ggcggcggtc tggtggatgg tgatgcgccg gtgaagaaag aggcgcgtca gccgttaaca
    26461 gccaaagact ggaaactggt gttccatcgt aaactgatcg gcgtttatct tgggcaattt
    26521 gcggtggctt ctacactgtg gtttttctta acctggttcc cgaactattt aacccaggaa
    26581 aaaggaatca cggcgctgaa agcaggcttt atgaccacgg tgccattcct cgcggcgttt
    26641 gtcggcgtcc tgctctctgg ctgggtcgcg gatctgctgg tacgtaaggg cttttcactg
    26701 ggctttgcgc gtaaaacgcc gattatctgc ggcttgctga tctccacctg cattatgggc
    26761 gctaactaca ccaacgatcc gatgatgatt atgtgcctga tggcgctggc attcttcggc
    26821 aacggttttg cttcgattac ctggtcgctg gtctcttctc tggcaccgat gcgcctgatt
    26881 ggtttaaccg gcggcgtgtt taacttcgcc ggtggtctgg gcggcatcac cgttccgctg
    26941 gtggtggggt acctggcgca gggttacggt ttcgcacctg cactggttta tatctccgcc
    27001 gtcgcgttga ttggcgcgct ctcttacatc ctgctggtgg gcgatgtgaa gcgcgttggc
    27061 taatctttca actgtggaat atgcgccata ccactgcgca taatccactt tcttaaagcc
    27121 ggtgcggtca ggcatcggct ttttactatc ttgccgaaga gttttaaacg cagtttgcgg
    27181 gtggcgcgcc agtaagcggt attgagcttc tctggctgct tcagtaacac cgaacgcaga
    27241 atgtctgcgc tatccagcgc atagctaatc ccctccagcg aactggcgct gataaatcct
    27301 gccgcttcgc caatcagaaa ggcgttgtcc ttaccgcaga caaaatcctg ccagcgtgag
    27361 ggaaacagca ccgtgcattt ttcgcttttc accgccttac caaactggaa ctgaaaggcg
    27421 ctcattttct ctttcagcgt cgtgaaacgc gtctgaccgt ctttcattgg ataagcaccg
    27481 ccaaagataa aataaccgtc tttgctgata ctccatgaat aacagttagt tatcgaatta
    27541 tcaaagatgc aggagtagaa cggcaccgga tgtttctctg cgaaccactg ctggatagcg
    27601 acatatttac ggatttgatg atccgggtag agatgtcgcc gcaccatcga gtttgcgcca
    27661 tcggcaccga ccagatagcg ggcagtaata tgttgctccc agccgtctgc acgaaaaatg
    27721 acatgccatt tatcatcctc acgccagatt tttcggcaca ggctatcgtg ataaacctca
    27781 acgctggcgg ggatcagcga tttcatccac aagtcgaaag catggcgatt aatattgata
    27841 tagcttcgct ggtagttacg cgtgagcgat gcggcgacgt cgacagtttt gacgctgaaa
    27901 atctgtggat tggcgatcac atcgacagga agcgtcagtc catcgcgaat aaaagatcgc
    27961 tgcgcgtccg gtgccagcag accgccgcaa ggtttgctga aaccttcagt accacactgg
    28021 tgctttttat ccagcgcgat cacctgcatt ttgcctgcta attttcgcgc caacgccgat
    28081 cctgccgggc cgaggccaat aatcgccacg tcgaaatgtt ccatcttttc actcacattc
    28141 atcacgatgg caaaagacta ttgtctgaaa gtgaagtcct tgtgaagtat gatggcggga
    28201 cccgttttgt atcaggaccg cgccatgaaa cagataacct ttgctccccg taatcacctg
    28261 ctcaccaata ccaatacctg gacgcccgac agccagtggc tggtatttga cgtgcgtcct
    28321 tctggggcgt cgtttaccgg cgagaccatt gagcgtgtga atatccatac cggcgaggtc
    28381 gaggttatct atcgcgcgtc acagggcgca cacgtcggcg tggtgaccgt tcatccgaag
    28441 tcagagaaat atgttttcat ccatggcccg gaaaatcctg atgaaacatg gcattacgac
    28501 ttccatcatc gacgcggagt cattgctgaa ggcggcaagg tgagcaatct cgatgcaatg
    28561 gatattaccg cgccgtatac gccaggagcg ctgcgcggcg gcagccatgt gcatgtcttt
    28621 agcccgaacg gagaaagggt gagctttacc tataacgacc atgtaatgca tgaactcgat
    28681 ccggcgctgg atttgcgaaa cgtcggtgtt gccgcaccgt ttggcccggt caacgtacaa
    28741 aagcagcatc cgcgtgaata cagcggtagc cactggtgcg tactggtgag caaaaccacg
    28801 cccacaccgc agcctggcag tgatgaaatc aatcgtgctt atgaagaagg atgggtagga
    28861 aatcacgcgc tggcgtttat tggcgacaca ctttcgccaa agggcgagaa agtgccggag
    28921 ctgtttatcg ttgagttacc gcaagatgaa gctggctgga aagcggcagg tgatgcgccg
    28981 ttaagtggaa cggaaaccac cctgcccgcg ccaccgcgcg gcgtcgtgca gcgacgttta
    29041 acctttaccc accatcgggc ttatccgggg ttagtcaacg tcccgcgcca ctgggtgcgc
    29101 tgtaatccgc agggtacgca aatcgcgttt ttaatgcgtg atgataacgg cattgtgcaa
    29161 ctgtggctta tctcgccaca gggcggcgag ccgcgccagt taacccataa caaaacggat
    29221 attcagtctg catttaactg gcatccgtca ggagaatggt tgggctttgt gctggataat
    29281 cgaattgctt gtgcccatgc gcaaagcggc gaggttgagt atttaaccga aaaccacgcc
    29341 aatccacctt ctgcggacgc cgtggtcttc tcgccggatg gtcagtggct ggcgtggatg
    29401 gaaggcggcc agctgtggat caccgaaact gatcgctaaa tcagttatca ggcattctgg
    29461 ccggtggaat aaccgcattg gtggcttgcg cgttggcttc gcttttttca acgcgcgagc
    29521 gcaccgagct gtctttgcgg aacacatccc acggcagtaa aagcgtgtcc atcacagcgg
    29581 taaacggcat atcgagaatc gccagcgact tagtgcccca gttggtttca tcgtcgccaa
    29641 tcatggttgc gctggcgcgg gtgccgggat acgttccttc tttccctccg gtatgggaca
    29701 tcacgctgga gcatcctccg agtaatgtca ttccgctgca tatcatgaac gctaacaaca
    29761 catttcttat cataataaat tcatctgttg atcgtgggtg ttggcctgat gagttatagc
    29821 gatcccttgc tgaaaataac atcatcatta cgtcgcactg tggcggctat cgcactttaa
    29881 cgtttcgtgc tgccccctca gtctatgcaa tagaccataa actgcaaaaa aaagtccgct
    29941 gataaggctt gaaaagttca tttccagacc catttttaca tcgtagccga tgaggacgcg
    30001 cctgatgggt gttctggcta cctgacctgt ccattgtgga aggtcttaca ttctcgctga
    30061 tttcaggagc tattgattat gcgtaacttt gatttatccc cgctttaccg ttctgctatt
    30121 ggatttgacc gtttgtttaa ccacttagaa aacaaccaga gccagagtaa tggcggctac
    30181 cctccgtata acgttgaact ggtagacgaa aaccattacc gcattgctat cgctgtggct
    30241 ggttttgctg agagcgaact ggaaattacc gcccaggata atctgctggt ggtgaaaggt
    30301 gctcacgccg acgaacaaaa agagcgcacc tatctgtacc agggcatcgc tgaacgcaac
    30361 tttgaacgca aattccagtt agctgagaac attcatgttc gtggcgctaa cctggtaaat
    30421 ggtttgctgt atatcgatct cgaacgcgtg attccggaag cgaaaaaacc gcgccgtatc
    30481 gaaatcaact aattccttaa ggccgcctgg cgcggcctga catctccatg ctcgccgtca
    30541 gggagcatat gcgaatcttc ggatttgcag gtacttactc gcttcttaga aggagaaatg
    30601 actatgcgta acttcgattt atccccactg atgcgtcaat ggatcggttt tgacaaactg
    30661 gccaacgcac tgcaaaacgc cggtgaaagc cagagcttcc cgccgtacaa cattgagaaa
    30721 agcgacgata accactaccg cattaccctt gcgctggcag gtttccgtca ggaagattta
    30781 gagattcaac tggaaggtac gcgcctgagc gtaaaaggca cgccggagca gccaaaagaa
    30841 gagaaaaaat ggctgcatca agggcttatg aatcagccat ttagcctgag ctttacgctg
    30901 gctgaaaata tggaagtctc tggcgcaacc ttcgtaaacg gtttactgca tattgattta
    30961 attcgtaatg agcctgaacc catcgcagcg cagcgtatcg ctatcagcga acgtcccgcg
    31021 ttaaatagct aactagctat tctctttgcc ccgccattct ggtggggctt tttttgtctt
    31081 tcttcttcaa caatgcgtta aatttcgcct gtttaagata tttcggcacg ttctttcccg
    31141 tgataatgtg cgctataagg caaatctgct tcaccgattc agccataatc cgtagcaatt
    31201 aaaaatgtta tctcaagaga aatggacgat gagtgatata gcattaacgg tcagtattct
    31261 ggctttggtg gcagtcgtcg gtttgtttat cggcaatgtc aaatttcgcg gcataggatt
    31321 aggtattggc ggcgtgctgt ttggtgggat catcgtcggc cattttgttt ctcaggcggg
    31381 gatgacatta agtagcgata tgctgcatgt tattcaggaa tttggcctga tcctgttcgt
    31441 ttatactatc gggattcagg tagggccggg cttctttgcc tcattgcgcg tctccggatt
    31501 acgcctcaac ctgtttgctg ttctgatcgt catcatcggt ggtctggtta ccgccatcct
    31561 gcataaactg tttgatattc cactgccggt agtgctgggg attttctccg gtgcggttac
    31621 caatacgcca gcgctggggg cagggcagca gattttgcgc gacctgggta caccaatgga
    31681 aatggtcgat cagatgggga tgagttacgc gatggcgtat ccattcggca tttgcgggat
    31741 tttgttcacc atgtggatgt tgcgggttat tttccgcgtc aatgtcgaga cagaagctca
    31801 gcagcacgag tcttcacgca ccaatggcgg cgcgctgatc aagactatca atattcgcgt
    31861 tgagaaccct aacctgcatg atttagccat taaagatgta ccgattctca acggcgacaa
    31921 aattatctgc tcgcgtctga aacgcgaaga aaccctaaaa gttccttcgc cagataccat
    31981 tattcaactg ggcgatttgc tgcatctggt gggtcagcca gcggatttac ataatgcgca
    32041 actggtgatt ggtcaggagg tcgatacttc gctgtccacg aaaggcactg atttgcgcgt
    32101 cgagcgtgtg gtggtcacca atgaaaacgt gctcggaaaa cgtattcgcg acctgcactt
    32161 taaagaacgc tatgacgttg ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc
    32221 cagtggcgat atcagcctgc agttcggcga tatcctcaat ctggtggggc gtccgtccgc
    32281 aattgatgcc gttgccaatg tgctggggaa tgcgcagcaa aaactgcaac aggttcagat
    32341 gctgccagtg tttattggca tcgggcttgg cgtattgtta ggttctattc ccgtctttgt
    32401 gcctgggttc ccggccgcgt tgaaactggg gctggcgggc ggtccgctga ttatggcgtt
    32461 gatcctcggg cgtatcggca gtatcggcaa gctgtactgg tttatgccgc caagtgccaa
    32521 cctcgcgctg cgggagctgg ggatcgtgct gttcctctcg gtcgttggtc tgaaatctgg
    32581 tggggatttt gtgaataccc tggtcaatgg cgaagggcta agctggattg gttatggtgc
    32641 cctgatcacc gccgttccgc tgattactgt tggcattctg gcgcggatgt tagccaaaat
    32701 gaattacctg accatgtgcg ggatgctggc aggttccatg accgatcctc cggcgctggc
    32761 gtttgctaat aatcttcatc caaccagcgg tgcggcggcg ctctcttacg ccactgtcta
    32821 tccgttagtg atgttcctgc gcattatcac cccccaatta ctggcggtgc tcttctggag
    32881 tatcggttaa ctcttttccg ggtggagtcg ccgtaaatgg tactccacct ggtattcgct
    32941 ggcatttctg aacatcactg aataattcaa aaactccccg ctctcgctat atgacaacga
    33001 ggtgatccgc agcagtggcg tgtgttccgc taccttcatt tgccgcgcca gcagtctatc
    33061 ggcgagcact ggcgtcaggc tttcataatt accgccaatc aaaatcccgc actcttgttc
    33121 aatatattca aacttcgacc cttccagatg ttgcagcgaa agattacgga acagttttac
    33181 cggcatatag ctgtcttcca gcatcagcgg tttcccttcc acaaaacgaa cgcgacggga
    33241 gaagtagatc tgctcgttga tttgaatccg taactggctg gcaatgaccg gaggcgcagg
    33301 catgatttca aaaatcagca cctggctggt gaccgttttt ccctgccgtt ttaacacctc
    33361 caccagcccg gtcaggctgg cggtttgatg cagtacatct ttgcgcacca ggtaagtgcc
    33421 gctgccgtgg cggcggacca ccaatcccca cgctaccagc aggtcaatgg ctttacggat
    33481 ggtcatccgc gataccgcaa attcttccgc caactttttt tcaccgggaa gaaggctatt
    33541 tagcgtgaaa tctgcagaat taagtcgaat tcttaaccgc tccgcaatgc ttttgtagat
    33601 catctctaga tctctttttt gcctgcaagc acgggtaatg cgcccacaag ttctgcttaa
    33661 tcgattgaaa aaacatcttt tttttaaaga tgtgttcgat gctgtgtacc tgtgctcaca
    33721 gatgtctact ttttcgcgaa aacgtagatc tctaccgccc aacgaaaagc atgaaagcga
    33781 tcacgaatcc cattgggtcg tcatgttctg ccgatcgcat cttcctatcc tcgctccagg
    33841 cctgccgcat aaccaatcag gcttcctact tacagaattg agaaaagagg atgtggaaat
    33901 gctcagtcaa attcaacgct ttggcggcgc gatgttcacg ccagtgctgc tgtttccctt
    33961 cgccgggatt gtggtgggtc ttgccatctt gctgcaaaac ccgatgtttg tcggggaatc
    34021 actgaccgat ccgaacagtt tattcgcgca aatcgtacac attattgaag agggcggttg
    34081 gacggtgttc cgtaatatgc cgctgatttt tgctgtcggt ttacccattg gccttgctaa
    34141 gcaagcgcag gggcgtgctt gtctggcggt gatggtgagt ttcctgacct ggaactattt
    34201 catcaacgcg atgggaatga cctggggaag ctacttcggc gtcgatttca ctcaggacgc
    34261 ggtggcaggt agcggtctga caatgatggc cgggattaaa accctcgata ccagcattat
    34321 cggcgcaatt atcatttccg gcattgtgac ggcgctgcat aaccgtctgt tcgataaaaa
    34381 actgccggtt tttctcggca ttttccaggg gacgtcttat gtggtgatta tcgccttcct
    34441 ggtgatgatc ccctgtgcct ggctgacgtt gctcggctgg ccaaaagtac aaatggggat
    34501 tgaatctctg caagcgttcc tgcgttcggc gggtgcgctt ggggtgtggg tttacacctt
    34561 cctcgaacgt attctgatcc caaccggttt acaccacttc atctacggac cgtttatctt
    34621 tggtccggca gctgttgaag gcggcattca gatgtactgg gcgcagcatc tgcaagagtt
    34681 cagtctgagc gccgagccgc tgaaatcgtt gttcccggaa ggcggttttg ccctgcacgg
    34741 taactcaaaa atctttggtg ccgtgggcat ttctttagcg atgtacttca ctgccgcacc
    34801 ggaaaatcgg gtaaaagtgg cgggcttgct gattcccgca accttaaccg ccatgctggt
    34861 gggaattacc gaaccgctgg aatttacctt cctgttcatt tcaccgttgc tgtttgcggt
    34921 acacgctgtg cttgcggcct caatgtcgac cgtgatgtat ctctttggtg tggtgggcaa
    34981 catgggcgga ggtctgattg accaggtttt accgcaaaac tggatcccga tgttcagcaa
    35041 ccacgcggat atgatgctga cccaaatcgc cattgggttg tgctttaccc tgttgtactt
    35101 cgtggttttc cgcacactga ttctgcagtt caacatgtgc acgccgggac gtgaagatgc
    35161 ggaagtgaaa ctctactcaa aagccgaata caaagcctcg cgaggccaaa ccaccgcggc
    35221 agagccaaaa aaagagctgg atcaggctgc cggtatcctg caagccctgg gcggggtcgg
    35281 caatatctcc agcattaaca attgcgcgac gcgtttacgt attgcactgc atgacatgtc
    35341 acaaacgctg gatgacgaag tctttaaaaa gctgggagcg cacggcgtct tccgtagtgg
    35401 cgatgccatt caggtgatca ttggtctgca tgtatcccag ctgcgtgaac agctcgatag
    35461 cttaattaat tctcatcaat cagcagaaaa tgttgccatt acggaggcag tataatgacc
    35521 aaattctcag tggttgtcgc aggcggtgga agcaccttta cgccaggcat cgtgttgatg
    35581 ctcctggcga atcaggaccg tttcccgctt cgtgcactga aattttatga taacgatggt
    35641 gcgcggcagg aagtgattgc cgaagcctgt aaagtcatcc ttaaagagaa agcgccggac
    35701 attgcgttta gttacaccac cgatcctgaa gtggcattca gcgacgttga ttttgtcatg
    35761 gcgcacatcc gcgtcggcaa gtacccgatg cgcgaactgg atgaaaaaat cccactgcgc
    35821 cacggtgttg ttggtcagga aacttgcgga cccggcggaa tagcgtacgg catgcgttcc
    35881 attggcggcg tcctggaact ggtggattat atggaaaaat attcaccaaa cgcctggatg
    35941 ctcaactact ccaacccggc agcaattgtc gcagaagcca cgcgtcgtct gcgcccgaat
    36001 gcgaaaatcc tcaacatctg tgacatgcca atcggtattg aaagccggat ggcgcaaatt
    36061 gttgggctgc aagatcgcaa acagatgcgc gtgcgctact acggcctgaa ccactggtgg
    36121 tcggcgatat cccgcagttt cagaaagggt taatgagtca gcaagtggcg gtggaaaaac
    36181 tggtcgtgga cgcctgggaa cagcgttcat atcagcacct gtggtaggcg attacgttgt
    36241 cgaaaacggt accgagcgcc tcggtcgcca aagctattct ggatgagttg ctggaggcca
    36301 acaaagcgta ctggccagag ttacgttaat tgtgtggact ggcatccgtt gggtgctggt
    36361 ccgtattctt tagcacggct gacggcgcat aaccaaattt atgtttaaag gctttgctga
    36421 aatgaaagga gtcgaagaag ttgagcgtat cagcgacttc gccaaccgag tttccttgtt
    36481 gctttaatag cgatagcgcc agatccagac gcgcatcgag gtaatattct ttcggcgtct
    36541 tgccggtata gcgtaaaaac agacgacgca accaggcttc gctacagggg atcgtggcag
    36601 ccatatcagc tacgctccag cgttgttgca gactggcatg taaagtggca attaattttt
    36661 caatttgccg ccgttgtgga tcttttttac catctgcgta cataagacaa atccactgat
    36721 aaataatttt cgtcagaaaa gcgactgcca gattattttt tattgcttct ggtgaagtta
    36781 ttaactcagc aacttcggtg agttcctgat tataaatttc gccattataa ataacgcttt
    36841 gctgaccaac gggaatatcc atcatgctgg tgggggtaaa ttccatccag tactgttccc
    36901 agactaaacc ttcacagtga taagagtgaa tatccattgg ctttaagaat ataatacagt
    36961 tgccatgcag tgttatttgt tcgccattct ttaaaagaat tttcccacat ccttgtacgg
    37021 tatacaccgc aacccatgag ttggcgataa gcggtttctt cttgtcccgc ggccagatca
    37081 cccggtaatg ctcgtcggcg taggtatgcc agagcgagat cagagaaatt ccgctggaga
    37141 tcacattttc gttaatcaat aagggaatat tgtacggagt ttcgtttttt acatctgaac
    37201 tttgcaattt tccattcatt ttaatatccc tccggcgaat tatgctcaca acctgaactt
    37261 aacagaaaac aatccgttca gggatacgac gtttgtattt taagaatctg actgcctgac
    37321 ccgacgcatt ttacctctcc ctatattcat gcgtccggga cataaacata aataagggct
    37381 atgagatgaa ttcgttacaa atcttgagtt ttgtcggttt tacgctgctg gtggcggtga
    37441 tcacctggtg gaaggtccgc aaaacagata ccggatcgca acaaggctat tttcttgccg
    37501 gacgttcact aaaagcgccg gttattgccg cttcgttaat gctaaccaac ctttccacgg
    37561 aacaactggt cggcctttcc gggcaggcct acaaaagcgg catgtcggtg atgggctggg
    37621 aagtgacttc agcggtgacg ctgatcttcc tcgcgctaat ctttttaccg cgctatctga
    37681 agcgcggcat tgccaccatc cccgattttc tggaggaacg ttatgataaa acgacgcgta
    37741 ttatcatcga cttctgcttc ctcattgcca ccggcgtctg ctttctgccg attgttctct
    37801 actccggcgc gttggcgctc aacagcctgt ttcacgtcgg ggaatcgcta cagatttctc
    37861 acggtgcggc tatctggcta ctggtaattt tgcttggtct ggcgggaatt ttgtatgcgg
    37921 tgatcggcgg actgcgcgca atggcagtgg cggactccat caacggtatt gggctggtta
    37981 tcggcgggtt gatggtgccg gtatttggcc tgatcgcgat gggcaagggc agctttatgc
    38041 agggcattga gcaactcacc accgttcacg ccgagaaatt aaactcaatc ggtggcccga
    38101 ccgatccctt gccgattggc gcggcattta ccggtttgat tctggtgaac accttttact
    38161 ggtgtacaaa tcagggcatc gtgcaacgca cgctggcgtc aaaaagcctg gcggaagggc
    38221 aaaagggggc gctgttaacg gcggtgctga aaatgctcga cccgctggta ctggtgctgc
    38281 cagggttgat tgcgtttcat ctgtatcagg atttaccgaa agccgacatg gcctacccga
    38341 cgctggtcaa taacgttctg ccagtgccaa tggtgggttt cttcggcgcg gtgttatttg
    38401 gtgcggtgat cagtaccttc aacggctttc tgaatagcgc cagtacgtta ttcagtatgg
    38461 gtatttaccg tcgcatcatt aaccagaatg ccgagccgca gcagctggtc accgtcgggc
    38521 gcaaatttgg tttctttatc gctatcgttt cggtgctggt cgcgccgtgg atcgccaacg
    38581 cgccgcaggg gctgtatagc tggatgaaac agctcaacgg catttacaac gtgccgctgg
    38641 ttaccatcat cattatgggc tttttcttcc cgcgcatccc ggcgctggcg gcaaaagtgg
    38701 cgatggggat tggcataatc agctacatca ccatcaacta tctggtgaag ttcgacttcc
    38761 atttcctcta tgtgctggcc tgtacgttct gcatcaacgt ggtcgtgatg ctggtgatcg
    38821 gttttatcaa accgcgcgcc acgccgttca ccttcaaaga tgcgtttgcg gtggacatga
    38881 aaccgtggag aaacgtcaag atcgcgtcaa ttggcatcct gttcgcgatg attggcgtct
    38941 atgccgggct ggctgaattc ggcggctacg gtacgcgctg gttagcgatg atcagttatt
    39001 tcattgccgc cgtagtgatt gtctacctga tttttgacag ctggcggcat cgtcacgacc
    39061 cagccgtaac ctttactccc gacgcgaagg atagcctatg aaacgcccca attttctgtt
    39121 catcatgacc gatacccagg ccaccaatat ggtcggttgc tatagcggta aaccgctgaa
    39181 tacgcaaaat attgatagtc tggcggcgga aggtattcgc tttaattccg cctacacctg
    39241 ttcaccggtt tgtacgcctg cacgcgccgg actatttacc ggtatctacg ctaaccagtc
    39301 cggcccgtgg accaacaacg tcgcgccagg caaaaacatc tccactatgg ggcgctactt
    39361 taaggatgcc ggctatcaca cctgttacat cggcaaatgg catctcgacg gtcatgacta
    39421 tttcggcact ggcgagtgtc cgccggagtg ggacgctgat tactggttcg atggggcgaa
    39481 ctaccttagc gaactgacgg aaaaagagat cagcctgtgg cgcaatggcc taaacagcgt
    39541 tgaggattta caggcgaacc atatcgacga aaccttcacc tgggcgcacc gcatcagcaa
    39601 tcgggcggta gattttctgc aacagcccgc gcgcgccgag gaacccttcc tgatggtggt
    39661 ttcgtatgat gagccgcatc acccgttcac ctgtccggtg gagtatttag agaaatacgc
    39721 tgatttttac tacgatctgg gcgagaaagc tcaggatgac ctggcgaaca aaccggaaca
    39781 tcaccgctta tgggcgcagg cgatgccatc gccagtcggt gatgacgggc tttatcacca
    39841 tccgctctat tttgcctgta atgactttgt tgatgaccaa atcggacggg tcatcaatgc
    39901 cttaacgcca gagcaacgtg aaaatacgtg ggtcatttat acttccgatc acggcgaaat
    39961 gatgggcgca cataagctga tcagtaaagg agcggcgatg tatgacgaca tcacccgtat
    40021 tccgctgatc atccgttcgc cgcaagggga gcggcgacag gtcgatacgc cagtcagtca
    40081 tatcgattta ctgccgacaa tgatggcgct ggcagatatt gaaaaaccag agattctgcc
    40141 gggggaaaat atccttgccg tgaaagagcc acgcggtgta atggtggaat ttaaccgcta
    40201 cgagattgag catgacagct ttggcggttt tattccggtg cgttgctggg tgacggatga
    40261 ctttaaactg gtactcaacc tcttcaccag tgatgaactt tacgatcgcc gtaatgaccc
    40321 aaatgaaatg cataacctga tcgatgatat ccgttttgca gacgttcgca gcaaaatgca
    40381 tgacgcctta ttggattaca tggacaaaat tcgcgatccg ttccgcagtt accaatggag
    40441 cctgcgtccg tggcgtaaag atgcactgcc gcgctggatg ggggcatttc gtccacgccc
    40501 acaagatggc tattcgccgg tggtacgtga ctatgacacc ggcctaccga cgcaaggagt
    40561 gaaagtggaa gagaaaaaac agaagttctg acgataatgc ccgctgatga acacccggcg
    40621 ggcattattc aggctgctta aaccagtgaa ccgccggtca ggaaggaaaa aatcaggaat
    40681 atcagcaaaa tgctgcacac gatgccgata ccaaaaagca aggtatggaa agccagcgtc
    40741 ccgccgccaa aaaccagcgc gccgcgaatg ccccagcgat tttccttacg cataccgcca
    40801 ataaatgcgc aaagtagcgc agcaatggcc agtgcgatac cgctttgatc gaggatttta
    40861 tcgacatcta cgtcctcttt tttctccacg gtggtaattt tttcgccttt tattccggcg
    40921 agaagacctc ttttaatttc agccgttttg tccgccacga tgctttcaaa cgttggcggt
    40981 ggtgcggaaa aagggccgaa tgaaaagtgg ataatcccca tcattaaagc gatggcaccg
    41041 aacagcatgc ccaatgaact gattttattt tgagctatga tacccatctg acctccttgt
    41101 agcggatggc gaaagcatat caggaaacgg gcggacgaaa ttcttaaaaa aaaaccgcta
    41161 aaggtagagc gttagtgaaa ttgcaaagga gcaacaatga aaatttcccg ccttggagaa
    41221 gcgcccgatt accgcttctc gctggctaat gagcgtactt ttctggcgtg gatccgtacc
    41281 gcactaggat ttctggcggc gggggttggg cttgatcaac ttgcgccaga tttcgccacg
    41341 cccgtcattc gcgaactgct ggcgttgttg ttgtgtctgt tttccggcgg gttagcgatg
    41401 tatggctact tgcgctggtt acgcaatgaa aaagcgatgc gtctgaaaga ggacctgcct
    41461 tacaccaaca gcttattaat catcagttta atattgatgg tcgtggcggt gatcgttatg
    41521 ggactggtgt tgtatgccgg atagccgcaa agccagacgc attgccgacc caggactgca
    41581 gccggagcgt acatcactgg cgtggtttcg taccatgctg ggctacggcg cgttgatggc
    41641 gttggctatc aaacacaact ggcaccaggc gggcatgtta ttctggattt cgattggcat
    41701 cctcgccatc gtggcgctga tcctctggca ctacactcgt aatcgcaatt taatggatgt
    41761 cacgaatagc gatttttccc aatttcacgt agtccgtgac aaatttttga tctccctcgc
    41821 ggtgttatct ctcgcaatac tgtttgctgt aacgcatata catcaactta tcgtatttat
    41881 tgagagagtc gcatgacagg aagtcaggtc attgatgctg aagaggacag gcataagctg
    41941 gttgtagagt ataaggatgc tctgcaacct gccgattttt atcacaattt caaacaacgg
    42001 ggcatccgct ccgtacagct cattccccat cttgaatttg atgaccgggg cgatctgacg
    42061 gccgcttcgg tgacagcaga gctgtggggg aaatttttaa ttgccctgtt cgagtgttgg
    42121 gtgcgggcag atatcagtcg tatatcgatt gagctttttg acgctaccct acaaaaatgg
    42181 tgtggcagcg aaaacccgca actgcgccgt gactgccagg cgtgcgactg gcatcggctg
    42241 tgtccgcacg ctcgggagga gacgccagac agcgtgctat gtgcgggcta tcaggcgttt
    42301 tattcttact ccgcaccgca tatgcgggta atgcgtgatc tgataaagca gcatcgttct
    42361 cccatggagc tgatgacgat gctgcggtga cgtgcgctta aacgggctgc ccctgatgcg
    42421 acatccgcgt cgccagcggc agccagcaca gcacgatcaa caatcccatt aaggtcatca
    42481 ataaccccag gctgccctga ccggtttgcg gcaacatcgc agagagcgac gccagcacgc
    42541 cggaaccaat gttttgcaga ccgccgacca gcgcgccagc cgtgcccgcc aggaagggga
    42601 acggctccat cgcgccgctg gtcgccagcg gaaacagcat cccggcaccg aaaaagaaca
    42661 gcgcggcggg aacgagcagc gtccagacat tcatcacgcc aaaccagtct gggatccaca
    42721 tcagcaagcc agccagcagg cagcagataa ccgactgcca cattaacgtg gagaagcgtt
    42781 tattgggacg tccggcaaac catgcgccaa aaaacgctgc cggaatcggc agaataaaca
    42841 aaatactgac cgtcatactg ctcagcccta acaccgcgcc catcagcacg ccggagcagg
    42901 cttcaaaggc ggcaatcccg gccagaccgc caatcagcat cagcaaataa cagttaaaac
    42961 cgctgttacc gaaaagcgtt ttataactgg taagcaggcg cgtgcgcggt gcatcgaccg
    43021 gacgcgtttc cggcatccag cgggccatac tgaaggtcac accagcacaa agaaccaaca
    43081 aaaagagata acaggcgcgc cagttccaca tcgtatccag cagaccgccg attagcggtg
    43141 cgagcaacgg actgacgaga atccccatgt ttaacaggct gttagcatgg cgcaactgtg
    43201 tccgttcata taaatctcgc ggcaaagtac gcgccattac gccgccaacg ccggtgccca
    43261 tcccctgcat cgcgctggcg gcaatcaaca ccgtcaaact ggaggtcgtg accgcgacca
    43321 gcgttgccag cataaaaatg gacattccga cgaggatcac cggtcggcgg cccacgcggt
    43381 cggaaatcgg gccataaaac agctgtgaga caccgtaagt cagcagataa gcgcccatta
    43441 cgctctgcac cgccccttca cggacgttga gatcgcgcgc catatcggca atagctggaa
    43501 tataaatggt ttgcgccatc tgaccgacgg ccacgagtaa taccaacatc aataacaaat
    43561 tgacgtttct ttgccttttc attatcacgg atgcttttat aaaaataatt agaataagtc
    43621 actacgcatt cccataaatg cgggagagga atctatcaca ttgatgaatg ttagccagat
    43681 atacgccagg aatggggaat tgtttagcgg cagaatatgt aaacaaaagc ggcaataaat
    43741 gttgccggga gggattacgt gcgaactgta accctctccc caaaggggcg agggggaaac
    43801 caccgttccc tttcagaaaa tgagctaaac attgcacggg acggtcccct cgcccctttg
    43861 gggagagggc tagggtgagg ggcacaggca gcgcaggtgc aaacattctg ctggtagaag
    43921 aagcccccaa ctccaacccg cacgctaaat ataaaagggg agcggttacc cgctcccctt
    43981 tggtgcgact tgaatctgaa ttacttcagg tatttcagaa cagcatcaag cagttgcagt
    44041 gctgcaacaa tgagtttgag gataagaatg gcgatatcca ccaggctcat acgcgtctcc
    44101 tgtggttcag gagcactgcc agctgacgta cctttccgct gcctgttgcc a
//
LOCUS       DAFATA010000023        43516 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFATA010000023 DAFATA010000000
VERSION     DAFATA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.023"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..881)
                     /locus_tag="LGJ20_001751"
     CDS             complement(<1..881)
                     /locus_tag="LGJ20_001751"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK2385820.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTN"
     gene            complement(881..2044)
                     /locus_tag="LGJ20_001752"
     CDS             complement(881..2044)
                     /locus_tag="LGJ20_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBK2385821.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(2128..2490)
                     /locus_tag="LGJ20_001753"
     CDS             complement(2128..2490)
                     /locus_tag="LGJ20_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK2385822.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(2507..3412)
                     /locus_tag="LGJ20_001754"
     CDS             complement(2507..3412)
                     /locus_tag="LGJ20_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385823.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(3681..4685)
                     /gene="trpS"
                     /locus_tag="LGJ20_001755"
     CDS             complement(3681..4685)
                     /gene="trpS"
                     /locus_tag="LGJ20_001755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK2385824.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(4678..5436)
                     /gene="gph"
                     /locus_tag="LGJ20_001756"
     CDS             complement(4678..5436)
                     /gene="gph"
                     /locus_tag="LGJ20_001756"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBK2385825.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHAADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(5429..6106)
                     /gene="rpe"
                     /locus_tag="LGJ20_001757"
     CDS             complement(5429..6106)
                     /gene="rpe"
                     /locus_tag="LGJ20_001757"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK2385826.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(6124..6960)
                     /gene="dam"
                     /locus_tag="LGJ20_001758"
     CDS             complement(6124..6960)
                     /gene="dam"
                     /locus_tag="LGJ20_001758"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBK2385827.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(7067..8353)
                     /gene="damX"
                     /locus_tag="LGJ20_001759"
     CDS             complement(7067..8353)
                     /gene="damX"
                     /locus_tag="LGJ20_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBK2385828.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(8445..9533)
                     /gene="aroB"
                     /locus_tag="LGJ20_001760"
     CDS             complement(8445..9533)
                     /gene="aroB"
                     /locus_tag="LGJ20_001760"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK2385829.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(9590..10111)
                     /gene="aroK"
                     /locus_tag="LGJ20_001761"
     CDS             complement(9590..10111)
                     /gene="aroK"
                     /locus_tag="LGJ20_001761"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBK2385830.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(10512..11750)
                     /gene="hofQ"
                     /locus_tag="LGJ20_001762"
     CDS             complement(10512..11750)
                     /gene="hofQ"
                     /locus_tag="LGJ20_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBK2385831.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(11662..12066)
                     /gene="hofP"
                     /locus_tag="LGJ20_001763"
     CDS             complement(11662..12066)
                     /gene="hofP"
                     /locus_tag="LGJ20_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBK2385832.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(12056..12496)
                     /gene="hofO"
                     /locus_tag="LGJ20_001764"
     CDS             complement(12056..12496)
                     /gene="hofO"
                     /locus_tag="LGJ20_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBK2385833.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(12480..13019)
                     /gene="hofN"
                     /locus_tag="LGJ20_001765"
     CDS             complement(12480..13019)
                     /gene="hofN"
                     /locus_tag="LGJ20_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBK2385834.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(13019..13798)
                     /gene="hofM"
                     /locus_tag="LGJ20_001766"
     CDS             complement(13019..13798)
                     /gene="hofM"
                     /locus_tag="LGJ20_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBK2385835.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            13918..16470
                     /gene="mrcA"
                     /locus_tag="LGJ20_001767"
     CDS             13918..16470
                     /gene="mrcA"
                     /locus_tag="LGJ20_001767"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBK2385836.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVKIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(16638..17198)
                     /gene="nudE"
                     /locus_tag="LGJ20_001768"
     CDS             complement(16638..17198)
                     /gene="nudE"
                     /locus_tag="LGJ20_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBK2385837.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            17518..19653
                     /gene="igaA"
                     /locus_tag="LGJ20_001769"
     CDS             17518..19653
                     /gene="igaA"
                     /locus_tag="LGJ20_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBK2385838.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            19718..20386
                     /gene="yrfG"
                     /locus_tag="LGJ20_001770"
     CDS             19718..20386
                     /gene="yrfG"
                     /locus_tag="LGJ20_001770"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBK2385839.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            20397..20798
                     /gene="hslR"
                     /locus_tag="LGJ20_001771"
     CDS             20397..20798
                     /gene="hslR"
                     /locus_tag="LGJ20_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBK2385840.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            20823..21701
                     /gene="hslO"
                     /locus_tag="LGJ20_001772"
     CDS             20823..21701
                     /gene="hslO"
                     /locus_tag="LGJ20_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK2385841.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(21836..23560)
                     /locus_tag="LGJ20_001773"
     CDS             complement(21836..23560)
                     /locus_tag="LGJ20_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBK2385842.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMVIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            23939..25561
                     /gene="pckA"
                     /locus_tag="LGJ20_001774"
     CDS             23939..25561
                     /gene="pckA"
                     /locus_tag="LGJ20_001774"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK2385843.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            25678..25995
                     /locus_tag="LGJ20_001775"
     CDS             25678..25995
                     /locus_tag="LGJ20_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2385844.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            26054..26350
                     /locus_tag="LGJ20_001776"
     CDS             26054..26350
                     /locus_tag="LGJ20_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2385845.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(26382..27734)
                     /gene="envZ"
                     /locus_tag="LGJ20_001777"
     CDS             complement(26382..27734)
                     /gene="envZ"
                     /locus_tag="LGJ20_001777"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBK2385846.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(27731..28450)
                     /gene="ompR"
                     /locus_tag="LGJ20_001778"
     CDS             complement(27731..28450)
                     /gene="ompR"
                     /locus_tag="LGJ20_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBK2385847.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            28678..29154
                     /gene="greB"
                     /locus_tag="LGJ20_001779"
     CDS             28678..29154
                     /gene="greB"
                     /locus_tag="LGJ20_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBK2385848.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            29251..31572
                     /locus_tag="LGJ20_001780"
     CDS             29251..31572
                     /locus_tag="LGJ20_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK2385849.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            32010..32237
                     /gene="feoA"
                     /locus_tag="LGJ20_001781"
     CDS             32010..32237
                     /gene="feoA"
                     /locus_tag="LGJ20_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBK2385850.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            32254..34575
                     /gene="feoB"
                     /locus_tag="LGJ20_001782"
     CDS             32254..34575
                     /gene="feoB"
                     /locus_tag="LGJ20_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBK2385851.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            34575..34811
                     /gene="feoC"
                     /locus_tag="LGJ20_001783"
     CDS             34575..34811
                     /gene="feoC"
                     /locus_tag="LGJ20_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBK2385852.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            35014..35892
                     /gene="rpnA"
                     /locus_tag="LGJ20_001784"
     CDS             35014..35892
                     /gene="rpnA"
                     /locus_tag="LGJ20_001784"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBK2385853.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(35921..36691)
                     /gene="bioH"
                     /locus_tag="LGJ20_001785"
     CDS             complement(35921..36691)
                     /gene="bioH"
                     /locus_tag="LGJ20_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBK2385854.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            36729..37412
                     /gene="gntX"
                     /locus_tag="LGJ20_001786"
     CDS             36729..37412
                     /gene="gntX"
                     /locus_tag="LGJ20_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBK2385855.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            37471..38046
                     /gene="nfuA"
                     /locus_tag="LGJ20_001787"
     CDS             37471..38046
                     /gene="nfuA"
                     /locus_tag="LGJ20_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBK2385856.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            38407..39723
                     /gene="gntT"
                     /locus_tag="LGJ20_001788"
     CDS             38407..39723
                     /gene="gntT"
                     /locus_tag="LGJ20_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBK2385857.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(39768..41852)
                     /gene="malQ"
                     /locus_tag="LGJ20_001789"
     CDS             complement(39768..41852)
                     /gene="malQ"
                     /locus_tag="LGJ20_001789"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBK2385858.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(41862..>43516)
                     /gene="glgP"
                     /locus_tag="LGJ20_001790"
     CDS             complement(41862..>43516)
                     /gene="glgP"
                     /locus_tag="LGJ20_001790"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HBK2385859.1"
                     /translation="NAEKLTKVLYPNDNHTAGKKLRLMQQYFQCACSVADILRRHHLA
                     GRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAWAITSKTFAYTNHTLM
                     PEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEKVWAKLAVVHDKQVHM
                     ANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTNGITPRRWIKQCNPAL
                     AALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANKVRLAEFVKVRTGIEI
                     NPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVPRVFLFGAKAAPGYYL
                     AKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIPAADISEQISTAGKEA
                     SGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVEQVKAILAKGYDPVKW
                     RKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYLVMADFAAYVEAQKQV
                     DVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        10341 a        11901 c        10952 g        10322 t
ORIGIN      
        1 atgttggtgc aaataaacgc cgtcggatgg gtgttaaatt cgttgagggt gatatccata
       61 atccgctggc tatccaccag attttgccag ctcacgccat aaggattgtt gacgatatct
      121 gccaccttac ccaccagcac ggttggcacg cctgcttcat acagtttttg tggcacctgc
      181 actttttcat cgacgccata gcccatatgc acgacctgga aaccgttgtc ataagcgcca
      241 gaacgcggcg cattgatacc aataaagcgc ccttctttgc tttctgcggc atcgagaatg
      301 cgttgactgt cggttaacag gccaccaaat gtaatgaccc gaccgacctg tacctgctca
      361 cgcacgatac gaccaatttt gattgcgtcg tcaaaagaga tcacagagag attggcggta
      421 atgttataga cctggcctaa atccgcctcg agattatcgc caatcgcaac cgcctgattg
      481 acccacagaa attgcagttc atcgccacgg cgctccacct gccaaccggc ggatactaat
      541 gcctgctcaa cacggtcaat cacatcgcaa aaaggcatcc gcagcggcgg taacgggcgc
      601 gtgcctaaaa tttcctgatg ccccataaag gtatcgccac cttcatgttg cagctctgcc
      661 acgccccagg ttgcggaatc tgacggctgc atatcgcctg gcgcataacc caatgcgttg
      721 attagcccca gcttctccag cgttggtagc tgcaaatgcg gcaactggct caggatgtga
      781 ccacaggtat tcgctcccgc atcttgcgga cgcaccagcg tgacatcttt cattgcccct
      841 acgccaaagc tatcaatcac taacaccaca aatcgcgcca ttacgccccc agagagtttc
      901 caagactgtc ataacgacca acgattttcg gttcgccacg gtgaataccg gacaccagca
      961 ccacatcgct acgggtgaca aaaatctgcg tgcggaaaca gagcaccact gcactgctca
     1021 ccggatactc gcctgccagc ggcagggtgt agtcgatact gctgtcatcc acagttttga
     1081 gattggtttc agtaatcttt tgattttctg gcgtaaacac cagcgcatgt tgcgcatgac
     1141 cacgacggta gtaaccgccg ccgtagcagt agctgtcgcc acggaaatga tgggagattt
     1201 cacttaacca gagcatcgcg atacgttcag gctgatcgcc ctgctggttt gccggaatag
     1261 tgcccgtcag cgcatgaccg ggttcggcat gagtcacacc gtattgcgcc agcaatggca
     1321 gcgaagtgca gctggtcgct gaaggcgcgt tcagttgctc aatcgcaata ccagatttcg
     1381 ccagttgatc ccgtgcttgt accagcgtgt gaagattcgg tgtcggcaaa actttcccgg
     1441 cagcctcatc ccaaagcagg caagggaaat gggttagtcc cgccaaatgc agccccggca
     1501 gtttcttaat ttcagcaacc acctcgtgca gcgaatgctg tacaaaaccg ctctcctgcc
     1561 ccggataaag aaaatcatcg tcgctataaa ctttgagcag cacagactgg attcgcccgg
     1621 ccttcaccgc cgccgcagaa acttcccgcg ctttgtcgag agtaaacacg gtgatgacgt
     1681 cggtgccctg ttcaacggcg tcagcaacct gatgacaagg gatttgtacc agatgcccct
     1741 gatgcgccac cggcaaacca gcgcggcgca tgactcgcgc ctctttgtaa tccaccgcca
     1801 caatgccgct gtagcccaac gccagcagtt tttccgccag ccatgggtta cgaccgaatt
     1861 gtttggtcat cagatacagt tcaatgccgt aaagccgcgc cgtctcaatc agccgcttac
     1921 cgttttccag tatctgatcc acgtcgatca cccagctgtc cggggcgatt ttgccctgct
     1981 gccacaaact tagtgcggcg gaaatcagcg ccgggttctg gcgtttcaat gcttctacaa
     2041 acatcttgtt gcctcgtttc tgttccttga attaaatatt catttttttg aatatttagg
     2101 ttgtgcgacg tggatttgtc aggagcttca aacctcttcg ttagcagcca tccataatcc
     2161 ataaaggttg gccagcaaat agccttcttc acacgggtta acttccagtg cgaattcctt
     2221 caacaacacc tgatgcaatt gcacaacggc ttgccagtgg ctggattgcg ccagctctgc
     2281 cagcaattcg ttatccagcg gctctatttc ttcaccacgg cgactgcgca tcagtgcact
     2341 cgccatatgt gtcatcgcca tcgttccttg ctcactgcgc accggcagat ggcactctgt
     2401 ttccagtacg ttgacgacct gcatcatgcc tttgcagatg tccttatcaa taacgcctgc
     2461 ctcgcaaagc aggttgagtc tggtttccat atcagatgcc tttcgtttaa taactcggtt
     2521 cctgttcccc actcactacc cgttgttgat gggcgagcag ggcgtgttta tctacaacgg
     2581 cacgcagcag ttgttgcatt gggtaatcat cgactcgcgt caggaccacg gcttcggtgg
     2641 cctgtaaaaa tcgcggatcg tctgtgagcg gcgtcgcctc taatcccagc atggtcagtt
     2701 cgttttccgc caccacgttc cagatcaccg catcgacatc gcctttaaca atgcgttgta
     2761 aactctcgtg ataagagaga tcgactcgtt ccacatcact accgccaaaa aaaacatcgg
     2821 tcatgatttt ctgatccgcc gaacggttat ccagccccac gcgcttcacg tttgcggact
     2881 cgcctttacg gcaaatcaac tggtgctcgc caacgtaggt gtgcggcccc aactccagcg
     2941 cgaggcataa ccctttttgc gtgagataac tttccgccgc cagtcgggaa accaccgcca
     3001 tgtcatacac gccgttaagt aaacactcca cgcgaatatc cgcgccgcgc atatgcgcat
     3061 agtaaaaagg aatgccatca aactgagctt tcagcccgct cgctaaacct tcgtacaagc
     3121 gggtataggg caaaggcatc gcacatacca cgttgttgat atccacatga ctcagcaatg
     3181 ctttgttatc catctcgacc agataactgc cattgcgccc acggcgttca atccgtatcg
     3241 ctccgcttga ttccagcgtt ttcaacgcgg cctgcgtcag gccaaccgat gaacggcatt
     3301 cagttgccag ctcatctatg gttttcaacc tattaccgca tttttcaccc aacaaataac
     3361 gggccagcgt cgtgataacg acgccttctt ttttgataaa cgttcgacgc ataatatatt
     3421 ttcaataaaa tgaatgttta tatcttcatt aaaatgaaga taagtggcaa ctgtgagatg
     3481 tggaaacggc gaggcgcttc acattttttc ggattattgc aaatgaggat aaaaaaaccg
     3541 ggtttccccg gtttcagagt gatgacgaag tgatttattc gacaatgcga gcaaagggta
     3601 attcccactg gcgcttgcct gctcccctca ccctaaccct ctccccaaag gggcgagggg
     3661 accgtccgag cgttttgata ttacggcttc gccacaaaac caatcgcttc gtacaccgct
     3721 ttcagcgtac gggaagcgtg tacactggct ttttctgcgc cgtctttcat cacctgttgc
     3781 aggaaggctt catcgttgcg gaaacgatga tagcgttcct gcaattcagt cagcataccg
     3841 gaaacggcat cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt
     3901 tccagttctg ggatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg
     3961 cccgctttgt tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgcg
     4021 cgtttgattt tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg
     4081 ttatcgtcag acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca
     4141 gatttcggaa taaacggctc cggcacctta aagatctcgc catacagcgc gttgaaacgc
     4201 tgggcgatat cgcgactcag ttccaggtgc tgtttctggt cttcacccac cggtaccaga
     4261 ttagtttgat acagcaggat gtccgcagcc atcagcaccg gatagtcaaa cagaccagcg
     4321 ttgatgttct cggcataacg cgcagattta tctttaaact gggtcatgcg gctcagttcg
     4381 ccgaagtagg tatagcagtt cagtgcccag cctaactgcg catgttctgg tacgtgggac
     4441 tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta
     4501 tccagcgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca
     4561 acgatacagt aaatgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc
     4621 atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta
     4681 gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt
     4741 tatagactga taaattacat caggctggct gagatcgata gcctcgccgt agttatatcc
     4801 gtaggttaag ccaacggatg ggcaacctgc cgcttttgcc gcctgaatat cattgcgtga
     4861 gtcgccgaca aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag
     4921 cgggtccgga tgaggttttt tgttttgcac atcatcgccg ccgataacca cgctaaagta
     4981 tttggcaata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac
     5041 caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcag cgtgcgggaa
     5101 caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat
     5161 acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttaccca ttgttttacg
     5221 ctgagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc
     5281 gttaccaatc caggtaataa cgcgttcttc acctgcgacg ggcaactcca gcgcatacag
     5341 cgccatatct accgcagcag caagaccggg agcactgtcg accagcgtac catcaagatc
     5401 aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc
     5461 gcatttcatc aatgactttt ttgtagtctg gctggtcgaa gattgccgaa ccggcgacga
     5521 acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcta
     5581 cttccagacg aatgtcaaag ccagactcgt cgatacgacg acgtacttcg cgcagtttat
     5641 ccagtgtttg aggaatgaac gactgaccgc cgaaaccagg attgacggac atcagcagga
     5701 tcacatccag cttatccatc acgtaatcca gatagctcag cggtgtcgcc gggttaaata
     5761 ccagtcccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg
     5821 aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc
     5881 gatcgacagg tttcaccatc aggtgtacgt cgataggggc ggtaatgccg tagttacgca
     5941 aggatttcag caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt
     6001 caaaatgcac gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg
     6061 caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga
     6121 gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac
     6181 ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt
     6241 tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat
     6301 atggcgctca accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa
     6361 actgtttgtg tgatacgccg taaagttggc ggtcgcagac agcggtgcat aaggcggatc
     6421 gcaatagacg acggatgcat catctgcgcg cgccatgcta tcggcgtaag actcacaata
     6481 gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg
     6541 ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc
     6601 gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct
     6661 tttgttgaac tcttcgcgga actgatagta gacctcggcg caatttgttt cgggaacaaa
     6721 cagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact
     6781 gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac
     6841 cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat
     6901 atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat
     6961 gctgactaac taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct
     7021 tcaggcaact cccgcaacga taacccattg cgggagataa ccttgattac ttcagatcgg
     7081 cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctgaacatct gctggcaatg
     7141 tagataccgc ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc
     7201 acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg
     7261 cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg
     7321 acggtgccga tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg
     7381 ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg
     7441 ctggcgcaga agtcgcagcc ggtgccttcg tgctcgccac aggagcagct ggttcagtac
     7501 gcttcggcgt ctgtgctacc ggcttcggtt ccgttttcac ggttgcttgc ggttttttcg
     7561 gttcaatcac cgcctgctga cgagctggac gcgtagtggc cggacgttca gcagtttgcg
     7621 ttttcgccgt gtcacgcgat gcattgccat tgcgtactgg cgcgacagtt gctggttcag
     7681 tcggcaatgt agaattgacc gccacattgt tcaactgttg ctgattttgt ggctgggtca
     7741 gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccgccgggg
     7801 tttgcccttg agtcggcgta gaagagatcg gtggcagaga aacatcctgc tgagtatttt
     7861 ccgcagacgt ggttcccggc gctggctgca ccccattcgc ctgatcggtc gcattgcctg
     7921 caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagaggtc gagggggctt
     7981 ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg acgcccatca
     8041 tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct
     8101 ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg
     8161 gttcctcatt gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt
     8221 caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag
     8281 attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt
     8341 tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct
     8401 gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa
     8461 tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca
     8521 attgccaacg gaagaattaa gcgtatctct cccgcaagga ctttcttgtc acgcagcata
     8581 tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc
     8641 ttgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac
     8701 gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac
     8761 cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac
     8821 ccggtttcgc gctcgtcggc ggcgacaact tctgccttca gttcacaaca acggcgaata
     8881 cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag
     8941 ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct
     9001 aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggctgg
     9061 tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg
     9121 gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac accgcgctga
     9181 taactcgccg ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc
     9241 gtagtatcgc gaccatgcgg cttttgtaac aacgccgtaa agacggtatc gagtacagcc
     9301 aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt
     9361 tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgtt ggtgaccaac
     9421 atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat
     9481 gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga
     9541 catccacctt aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt
     9601 ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg
     9661 tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt
     9721 cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa
     9781 gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac
     9841 gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg
     9901 tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga
     9961 aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt
    10021 ccatattgag ttgttgagct aactggcgcc caatagtgct ttttccggca cccataggcc
    10081 caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta
    10141 atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata
    10201 ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat
    10261 cacccatccc tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc accgctctgg
    10321 cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa
    10381 atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta
    10441 tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcataaaaa cattaccgct
    10501 tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt
    10561 cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc
    10621 aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac
    10681 gtttctccgc ttttgacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact
    10741 tcgccatcgg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc
    10801 cggatgcgac ctttttgtaa caccgtgggc gtgacctcca tccccaggac ggcctcttta
    10861 aattccaccg acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctcccc
    10921 tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc
    10981 agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gcaagcgtcc gttgatgcgc
    11041 ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg
    11101 gtgacttgcc caacaccacc agcgtgttgc gcatcggcca gcgtccattt cacgcctaac
    11161 tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac ctgcccgacc
    11221 ggaagatcca tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgt
    11281 agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc
    11341 tcccccgctt tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc
    11401 agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat attgtcattc
    11461 tgccaggcaa tagaatgcac tgagagaatg ttgccctcct ggcgcgttat cagcccggcg
    11521 cttttcacta cagtttgtag tgcctgcttc cagggaacat ctgttagatg taacgacacc
    11581 gtaccgctga cgtctggcga cacgaccagg ttcaacttct cctgttcagc cagcgcctgc
    11641 aacacctgag ccaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc
    11701 tgtacgccgg gtatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc
    11761 ttgccgttgc cacaaccatt gtggcggttc gcagtttgtc ccggtaccca gcgttagtac
    11821 gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc acatcgtttt gctgcacccg
    11881 tcgccatttc ttttgcccgt cttttattac accgatgatg cgctcgcctc gccctaccat
    11941 cccctgatag cgccactggc taagttcgct aatccggcat agatcttccg gcggtttaaa
    12001 agggtcacgc ataccggtta aaaggcacaa tgcaatacct gccaacaacc agcgtttaac
    12061 cctcatgcgg cgtctccagt tgtagcgtga acaaaagatc atcaccttcc acgcttaacg
    12121 aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt
    12181 cccacagcgt tttcaacgcc aactcgcctc cctgcgcgga tggatgccag gaaaccagtt
    12241 gcgcgccgga taactgaaaa tccagtggcg aaaagggcag cgttttttcc tcgctgaagg
    12301 gagtggtgtc taccaggcga tacagtgcgg cccactgctg gtgatgactt gcccgtagtc
    12361 gaattaatgc gtcgcgctct tcatggtgtg tcgatgatag aaaaatgagc gtaactaaca
    12421 tcagcaacca gaatgcccag caaagctggc ggaggcgggg tgatgtggcg aaccaccagt
    12481 caaagaacat gttcatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc
    12541 tgcgcatcct gctgcgtggc tccccgctga ttgagatgaa aagacgcatc ctggcggagt
    12601 gacgtttcta gtgcgtttaa cgcggtaatg cttgttgtca gccccttgat ctccagcgtt
    12661 ccctgctgcc agcttatcgt tgtcagccag gcgtgctcgg gtaaaagcgc cgccagtgct
    12721 tccagcgcag attgccagtc gcgggtaaat tgtcgctggc gctgccgttg cgaacgctgt
    12781 tcgcgtaact gctgttgctc cagcaaacgt ggcttcgtta tctgtaagct gcgggcgagt
    12841 tgttgttccg cctgaagcaa aactgcgtct atgcgcgctt cggcgctgcc tgtcagacgc
    12901 agtattagcg ttatcccgac ggccagcagc agaggcgcaa cgaacatcag caaccagaaa
    12961 cgcagaaaag cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc
    13021 agtacgcctt cccaagcgcc agccccagcg cgatggcaaa gtctccacct ggcggcggta
    13081 gcggcggctg acgaacagaa acggcctccc agggatcaaa tcccccttcg ccacatatcg
    13141 cgacgctttc gggatcaacg gataacgctg ccgccagctc cttcgcgcta gtcatcccca
    13201 ccgccagttt gcgcccccag cgacagcgtg tcgcccacag ccactgttcg ttatcacgcc
    13261 aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa ttgctgtaat gcactggcat
    13321 ccggggtgat cgcgctcaca tgaacacgca acctttctgc cagagtaagt agcgttgcca
    13381 gctctttgct ttgcgcggca gtcacgttat aggcggggct gagtgagtct tcgctgtaat
    13441 cgaagcgcag ggagtccgga tccatatcca gctcgcgggc catcgtccct gacagccagg
    13501 ccgtttgctc ccgctcacca agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc
    13561 gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcgcga ctccacggta
    13621 acaacgtttt agccagctgc tgcgcatcaa caatccgccc atctttgata atgtcgttct
    13681 ccagcggcaa ccgccaccag cgttgcaaaa agcattcttt tgctccccgc acgatcgcaa
    13741 ccgctaccgc ttcttgctgt tgtaaatgca aaccaatttg ccagatctta aatgccattg
    13801 tgatgatctc cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt
    13861 atactaccgc gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg
    13921 aagttcgtaa agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg
    13981 atttatggcc tataccgcta catcgagcca caactgccgg atgtggcgac attaaaagat
    14041 gttcgcctgc aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac
    14101 ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc caccggagat ggtgaaagcc
    14161 tttatcgcga cggaagacag ccgcttctac gagcatcacg gcgttgaccc ggtggggatc
    14221 ttccgtgcgg caagtgtggc gctgttctcc ggccacgcgt cacaaggggc gagtaccatt
    14281 acccagcagc tggcgagaaa tttcttcctc agtccagaac gcacgctgat gcgtaagatt
    14341 aaggaagtct tcctcgcgat tcgcattgaa cagttgctga cgaaagacga gatcctcgag
    14401 ctttatctga acaagattta cctcggttac cgcgcctatg gtgtcggtgc tgcggcacaa
    14461 gtctatttcg gtaaaacggt cgaccaactg acgctgaacg aaatggctgt gatcgccggg
    14521 ctgccgaaag cgccttccac ctttaatccg ctctactcaa tggatcgtgc cgtcgcgcgg
    14581 cgtaacgtcg tactgtcgcg gatgctggat gaagggtata tcacccaaca acagttcgat
    14641 cagacacgca ctgaggcgat taacgctaac tatcacgcgc cggagattgc tttctctgcg
    14701 ccgtacctga gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat
    14761 gaagacggtt atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcacagcag
    14821 gcggtacgta ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat
    14881 gtgctgtgga aagtgggcga gtcggcgtgg gataacaaca agattaccga tacgctgaag
    14941 gcgctgccaa cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcagcaa
    15001 gcgacggcga tgctggcgga cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg
    15061 gcgcgtcctt accgttcgga tactcagcaa ggaccgacgc cgcgtaaagt gaccgatgtt
    15121 ctgcaaacgg gtcagcaaat ctgggttcgt caggttggcg atgcatggtg gctggcacaa
    15181 gtgccggaag tgaactcggc gctggtgtcg atcaatccgc aaaacggtgc cgttatggcg
    15241 ctggtcggtg gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt
    15301 caggtgggtt ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctgacg
    15361 ctggcaagta tgttgaacga tgtgccaatt tctcgctggg atgcaggtgc cggttctgac
    15421 tggcagccga agaactcacc accgcagtat gctggtccaa ttcgcttacg tcaggggctg
    15481 ggtcagtcga aaaacgtggt gatggtacgc gcaatgcggg cgatgggcgt cgactacgct
    15541 gcagaatatc tgcaacgctt cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg
    15601 gcgctgggtt cagcgtcctt caccccaatg caggtggcgc gcggctacgc ggtcatggcg
    15661 aacggtggct tcctggtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc
    15721 gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc agtgatttac
    15781 ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc
    15841 cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta
    15901 gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca tcaacactcc gctggcattc
    15961 ctgattaaga gtgctttgaa caccaatatc tttggtgagc caggctggca gggtaccggc
    16021 tggcgtgcag gtcgtgattt gcagcgtcgc gatatcggcg ggaaaaccgg taccactaac
    16081 agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt
    16141 ggctttgatg atcaccgtcg taatctcggt catacaacgg cttccggagc gattaaagat
    16201 cagatatcag gttacgaagg cggtgccaag agcgcccagc ctgcatggga cgcttatatg
    16261 aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc cgccgccagg tattgtgacg
    16321 gtgaaaatcg atcgcagcac cgggcagtta gctaatggtg gcaacagccg cgaagagtat
    16381 ttcatcgaag gtacgcagcc gacacaacaa gcggtgcacg aggtgggtac gaccattatc
    16441 gataatggcg aagcacagga attgttctga ttaaaaaggc gcttcggcgc ctttttcagt
    16501 ttgctgacaa agtgcacttt gtttataccg gatacggcgt gaacacttta cccgtctagc
    16561 aacatctaga aaaatcaata tattgcagaa acttcgtaag cctgataagc attatgcatc
    16621 aggcgaactt tacgcattta cactcgcccc tgccctttca accattcacg cacgaggaac
    16681 agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc
    16741 gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg
    16801 tagagatctt gcgctaccac gatattcatt ttgctggaaa aataagacgg tgccatgctg
    16861 agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt tagctcgcgg
    16921 ttagcggctt cgtagacgct ttcacccgga tcaattaaac ctttcgaaaa acctaattcg
    16981 taggattcag ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc cacaatcggc
    17041 acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca
    17101 ttgctgaact ccagatccac gctctcgacg gtaaacagtc gggaacgggc tacagtttca
    17161 acattcagaa tggtgggttt ttgtaatgat ttgctcatcg tgggatctat gctgtgaaat
    17221 cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca
    17281 tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata
    17341 actccaggaa tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt
    17401 gatgtgctta atgatgctca agttaaactc cacgcttgcc gatagccaac cgcagaatca
    17461 tgtattgtgt ctggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg
    17521 agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg
    17581 ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa
    17641 acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg
    17701 caggtattac aggtgcctgg cccaacggga gctagcgcgg caccgatctc tctggcgctg
    17761 aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacgcgtta cggcatctct
    17821 accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgccccct
    17881 ttctgggaac aatacatcaa cgatgagaat accgttgaac tgatttatac cgattcgctg
    17941 ccgctggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga aacgcgcggc
    18001 tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga gagtgagcaa
    18061 atcgagctac tcaatattcg caaagaaacg catgaagaat atgcgctgag tcgtccgcgc
    18121 gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt ctgcctgatt
    18181 accccggatg tatttgttcc gtggctggca ggcggcgcat tactgctgct gggcgcaggt
    18241 ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc gagaaattca ttgtctgcgc
    18301 ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt
    18361 tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat tgctcaggat
    18421 ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg tcaggggcga
    18481 tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg
    18541 attatcgctg cgggctcgct gctggtgctg tttatgctgt tattctggat cccgctggat
    18601 atgccgctga aattcactct ctcatggatg aaaggcgcgc agaccattga agccaccagc
    18661 gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat tagcggtacg
    18721 ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc acctttttta
    18781 ccgtttgact gctcgcagat catctggaat gacgcccgct cattaccatt accagaatct
    18841 gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct gcaccctaaa
    18901 ccggaagatg aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa atccggcatg
    18961 gtattgcttg atgattttgg cgacattgta ctgaagacag cggatttatg ttctgccaaa
    19021 gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac
    19081 gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt attacgcccg
    19141 gtgagtgcgg aatcgctgga taacctggtg gcaacctcca ccgcgccgtt tatcacgcat
    19201 gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc
    19261 agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac
    19321 ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg
    19381 tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatatt
    19441 ggccttcatc cgatcccgga tcgttccggc ctgtggcgct atctcagcac cacattgctg
    19501 ctactgacga tgctgggtag cgccatttac aatggcgtac aggcctggcg tcgttaccag
    19561 cgtcatcgca ctcgcatgat ggagattcag gcctattatg aaagctgcct gaacccgcaa
    19621 ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc
    19681 ctgtcgcgca aattgcttca ctctggagat ttccctcatg catatcaaca ttgcctggca
    19741 ggacgtagat accgttctgc tggatatgga cggcacattg ctcgacctcg ccttcgataa
    19801 ctatttctgg caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg ttacgccaca
    19861 ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta
    19921 ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat
    19981 gggaccgcgt gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag
    20041 cggtaagcag cgaattttgc tcaccaatgc gcatccgcac aacctggcgg taaaacttga
    20101 gcataccggt ctggacgcac accttgattt attactttcc acccacacat ttggttatcc
    20161 gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag
    20221 aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat ttggtattcg
    20281 ttactgcctc ggcgtgacta atcctgattc cgggattgcc gagaaacagt atcaacgcca
    20341 tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga gacgccatga
    20401 aagaaaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc cgtttttata
    20461 aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc
    20521 gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg
    20581 acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag
    20641 ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgatggcac
    20701 gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa aaagagcgcc
    20761 gcgacctgtt acgatttaaa cacggcgaca gtgaataact gtcacctgca agagagatga
    20821 ttatgccgca acatgaccaa ttacatcgct atctgtttga aaattttgcc gttcgcggcg
    20881 aactggtaac cgtttcggaa accctgcaac agatccttga gaaccacgat tatccgcagc
    20941 ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga
    21001 agtttgatgg tgatatcacc gtacagctgc aaggcgacgg tccgatgaat ctggcggtta
    21061 ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc gaaattccag
    21121 aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc attaccccga
    21181 gcgaaggcga acgctatcag ggcgtggttg gtctggaagg tgataccctg gcggcctgcc
    21241 tggaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg
    21301 acgtagacgg caaaccggct gcaggcggta tgttgttgca ggtaatgcct gcgcaaaatg
    21361 cccagcagga cgactttgac cacctggcga cgctaaccga aaccatcaaa accgaagaac
    21421 tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg
    21481 tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgatg
    21541 cgctgaaaac gctgcctgat gaagaagttg atagcatcct ggcggaagat ggcgaaattg
    21601 acatgcattg tgattactgc ggtaaccact atctgttcaa tgcgatggat attgctgaaa
    21661 tccgcaacaa cgcgtctccg gcagatccgc aagttcatta atgagtatgt ccggcagaga
    21721 gggctctgcc ggattttacg acgtaatggc ctgatgtgct gcgcttacca ggcctgtgaa
    21781 ataaatcgca aattattgtt ggtcagatac agcgtcgtat ccgttaaaag agttgtcact
    21841 tacttaagtt tatttccagc gtttcaccat ctaccgtcag atcgcgccac gctttcggcc
    21901 tcgtccccag ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt atttcgttcg
    21961 gaagtaaact catatcaagc gcgtcccatt cttttctgtc tgagtcaaag ccatagacaa
    22021 tgactctgtc atcattaaaa gcaaacagga tccgctccgc ctgaccatca ctattcagat
    22081 cttgctcgac caggacgcag gcgtcttttt caatacaggt catcacgtta tagcgatcct
    22141 ggattaaggc cgaccagaat gtcgcatccg gtttaacaga accaggggca attaacacat
    22201 tttcggctaa tactttttcc gatacctgtt cctgaagatg ttgctctcca tctaacgcca
    22261 tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc ggcatcgctt ttcagcgact
    22321 caagcgccgc acgtccatag cgaccgctct gttcgagcat gtagatagtt acctggtctg
    22381 gcgtgttttt gccgctctga taacgcgcca tatgactgtt cacgctaata cgcatactgt
    22441 ccagcaccgg cgaattaaga agcaccagta tcaccaacac caataacgaa accgcaaggt
    22501 tcactttgcc ctgaagaaca aggggatttt gcccattacg ccagacgata ctgacaaaat
    22561 accccagcga ccacaccagt aacaccagca ccgccagcgc gccctgcaag cggtcgacag
    22621 tccagccgta ctgagcgacc cgcagccata acgcccaggc ggcgacgaac acatacagcg
    22681 gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg ccccgtccag ggaagtgacg
    22741 ctttttgcgg atcgcggaca ataaccatta agatcaattg caaaaaggcc agcgttaaca
    22801 gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac ggcagagtga
    22861 tgataaacat cagggttagc aatgatacta acggcagcaa ccccgtggcg attaacgtga
    22921 acaacttttg aatagagtcg attaaacgtg actgtgtgcg cgcgagaatc accgccagtg
    22981 cggtaaccag acctaatgta agatacataa accagtcggt tgcaaaaaag agcgtattga
    23041 aaaacttgat gccaacaagt ttaaacaact caccccacag taaaagcacc agccacgtca
    23101 agccattggc gagaaaaatc accagtaata taagtacgtt atgccatact gactggtaaa
    23161 aatagcgata gcggctactg tcattgcgga tgcgaaggct ttgttgtatc cacggtagca
    23221 acaacattgc catcaacagc agatagcagc caaaatccca aatcgccttt tcagctctcc
    23281 aggggttcat gccatcagtt tgccacttca accagccgct catccccagc gtggcaataa
    23341 acaccagcgc cagccatccc caaaggcgct tctgtttaaa agagatcacg gagaagagta
    23401 aaacggatga aaacgcaacg gtcgcaggca cgccataaac aatccaactg tgattttttc
    23461 cggcaagcca ggcgatcaac aggtagcaaa ccagcccctg aagtaatccg gtagcaatca
    23521 tcccccagcg tgttgccggt gaaagttcaa cgttatccat gttggttatc cagaatcaaa
    23581 aggtgggtta attatcgcat ccgggcagta gtattttgct tttttcagaa aataatcaaa
    23641 aaaagttagc gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattaagga
    23701 atgcgattcc actcacaata ttcccgccat ataaaccaag atttaacctt ttgagaacat
    23761 tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg
    23821 gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt
    23881 aacaccccca aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat
    23941 gcgcgttaac aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca
    24001 tgatatcgtt tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct
    24061 gacaggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat
    24121 cttcaccggt cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac
    24181 tttctggtgg gcagacaaag gcaaaggtaa aaacgacaac aaacctctct ctccggaaac
    24241 ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt
    24301 cgacgctttc tgtggtgcga acccggatac tcgtctttcc gtccgtttca tcaccgaagt
    24361 ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc
    24421 aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa
    24481 agaacagggt ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct
    24541 gattggcggc acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta
    24601 cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gtgagaaagg
    24661 cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc
    24721 gaaacgtcgc ctgattggcg atgacgaaca cggctgggac gatgacggcg tgtttaactt
    24781 cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta
    24841 caacgctatc cgtcgtgatg cgttgctgga aaacgtcacc gtgcgtgaag atggcactat
    24901 cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat
    24961 cgataacatc gttaagccgg tttccaaagc gggtcacgca acgaaagtta tcttcctgac
    25021 cgcagacgcc tttggtgtgc tgccgccggt ttctcgtctg actgccgatc aaacccagta
    25081 ccacttcctc tctggcttca ccgccaaact ggccggtact gagcgtggca tcaccgaacc
    25141 gacaccaacc ttctccgctt gcttcggcgc ggcgttcctg tcgctgcatc cgactcagta
    25201 cgcagaagtg ctggtaaaac gtatgcaagc ggcaggtgcg caggcgtatc tggttaacac
    25261 tggctggaac ggcactggca aacgtatctc gattaaagat acccgcgcta ttatcgacgc
    25321 catcctcaac ggttcgctgg ataacgcaga aactttcact ctgccgatgt ttaacctggc
    25381 gatcccaacc gaactgccgg gcgtagacac gaagatcctc gatccgcgta acacctacgc
    25441 ttccccggaa cagtggcagg aaaaagccga aaccctggcg aaactgttta tcgacaactt
    25501 cgataaatac actgacaccc cagcgggtgc tgcgctggta gcggcaggtc cgaagctgta
    25561 atcagctgat atctcattga ccccttatcc aaacggataa ggggtttttt tatatctgat
    25621 tagctatcaa tatgtacacg gtacacttat aataatcaat agaccgcagg atgcattatg
    25681 ttcaccttta ttgaactaca aggatttagc aaacgccgcc agttattgtt acctgatgat
    25741 gagttccggg cttttcagga actactcatt caagatccca ctgccggtga cattatcgca
    25801 ggcacaggca gttttagaaa aattcgctgg aaccgttctg gaatggggaa acggggtggc
    25861 attcggataa tttattacaa cgtaacacgc aatggccgaa tttatctggc gttgatttat
    25921 cctaaaaatg aacaggatga tttaacagaa gaacaaagaa aggcattgaa actattgtca
    25981 gagaagctgc tttaatgttg aagctccaca aattccacca tggtcgaaat gccaacggag
    26041 acattgaaaa cagatgaaag atgaattatt tgctgatctt cttgccagcg cagaagaaat
    26101 ggtacgcatc gaaaagggtg aagaaacgcc tgaaccagag cacgtccata cttttagcga
    26161 gatcgatgta aaagcgatcc gtgaagcaac gggtttacgt cagcaagatt ttgctgtcgc
    26221 cgtaggggta agttatgacc tggtcaaaag ctgggaaacc aaacgccgtc agcctaccgg
    26281 agcacctcga aaattgctcc tgcttttgca agcgaatcct ttcattatca atcaattgaa
    26341 aacaatctaa aaacgggaga caccttcgcc tcccgtttta tttacccttc ttttgtcgtg
    26401 ccctgcgccc gcgttaccgg cactggcagc caggcgcgaa tggaaagccc gccccgctcg
    26461 ctggtgccaa gctccagcat cccgttatgg ttatccacga tacgctgcac aattgccagc
    26521 cctaatcccg tgccgctaat ggtgcgcgca ctgtcgccgc ggacaaacgg ctggaacagg
    26581 tgcttacgtt gttccggcgc aattcccgga ccgtcatctt ccacctggaa ccaggcgcga
    26641 ttcggctccg ttccgctgct gactttgatc cagccattgc cataacgggc ggcgttgacc
    26701 accatattcg ccaccgcgcg tttgatcgac agcgggtgca ttttcacttc aatgctgccg
    26761 gggtaaagcg cggtttcaat ttcccgctca tagccacttt cggcagcaat cacctcaccg
    26821 agtactgcat taagatccgc catttccatc ggcatctcct gcccggtgcg caggtagtcg
    26881 ataaactgct caatgatggc gttgcactct tcgatatctt tattgatcga ttctgccaga
    26941 tagccatcct gctcgctcat catctcagtc gccaggcgaa tacgcgtcag cggcgtgcgc
    27001 aagtcgtgac ttacccccgc catcagcagc gtgcggtcat ccgccagttg cttaacacca
    27061 gccgccatat ggttaaaggc acgggtaacg gaacgcacct ccgaagcgcc atattcacgc
    27121 agcggcggcg gaataatccc tttaccaacc tgcaaggctg cgtgttcgag atcgaccaac
    27181 ggtcggttct ggatacgaat aaacagccac gccccgccta tcgccaatag cataatcgcc
    27241 agcgtatagc ggaacagcgg agagaaatcg ccctgatgaa tttcggtcag cggcacgcgt
    27301 acccagatat tgggcgacag ccaggttttc agccagacga caggcgaact tttgttgacc
    27361 tcaacgcgca cttccgtcgg gccgcccagt tgctgtgcca tctgatggct taagaattca
    27421 tagtgttgcg cccaacgcag acctgcctct tcggcagcct cgttggagta gagagagatc
    27481 cccagctcac ggtagatctc ccgacggaaa gcgggaggca caaccaactg cgtgccgtcc
    27541 tccagttgca gtttgtcggt catcaacata cgcacttcgt acgcgaggac tttattaaac
    27601 tgctggaggc tcggcaaaat cgcgaagttc agcaccacca gataagttgt caccaggctg
    27661 gcgaacagca aggtgacgat gagcaataac gtacgggcaa atgaacttcg tggcgagaag
    27721 cgcaatcgcc tcatgcttta gagccgtccg gtacaaagac gtagcccagg ccccaaacgg
    27781 tctgaatgta acgcggatgc gctggatctt cttctaccat gcggcgcaga cgcgaaatct
    27841 gcacgtcgat ggagcgttcc attgcggaat attcacgacc acgggcaagg ttcatcagct
    27901 tatcgcggga gagcggctca cgcggatggc tgaccagtgc cttcagtacc gcaaactcac
    27961 cgctggtgag cggcatcggc tcgtcttcgc ggaacatttc gcgcgtaccg aggttaagtt
    28021 tgaacttacc gaaagcaatt accgcctctt cctgtgatgg tgcgcctggt agctcgttcg
    28081 cctgacgacg cagcaccgca cggatacggg ccagcagttc acgcgggtta aacggttttg
    28141 gaatgtaatc gtcagcgcca atctccaggc caacgatacg gtccacttct tcccctttcg
    28201 ccgtcaccat aatgatcggc atcgggttgc tctgactacg aagacgtcgg caaatcgaca
    28261 agccatcttc accaggtaac atcaaatcca gtaccataag atggaaagat tcacgagtca
    28321 gcaggcgatc catctgttct gcattagcga cgcttcgaac ctggaagcct tgttcggtga
    28381 gataacgttc cagcagcgca cgcaggcgca tgtcgtcatc gaccaccaga atcttgtagt
    28441 tctcttgcat tgtttgtact cccaaaggtt cgcaacaatt tgtaagcgtg tattcttaaa
    28501 aaagctcacg ttcgtcacca gctaaatctg gtatgaattt cagcctaaat tgttacaaag
    28561 catattaaac agcagcttaa gtatacaatt tattcggcga aacattattg attctgttga
    28621 tatgatcacg ttatacccaa tgtgcgcatt atcaaacaga caaagggaat caacgagatg
    28681 aaaacgcccc tggttacccg ggaagggtat gaaaaactca aacaagagct taattatctc
    28741 tggcgtgaag aacgcccgga ggtcacaaaa aaggtgacct gggccgcaag tctgggcgac
    28801 cgcagcgaaa atgctgacta tcagtataat aaaaagcgcc tgcgtgaaat cgaccgtcgc
    28861 gtgcgctatc tcactaaatg catggaaaat ctcaaaatcg tcgattactc cccgcagcag
    28921 gaaggcaagg tcttttttgg cgcatgggtg gagattgaaa acgacgatgg cgtgactcac
    28981 cgtttccgta ttgtcggcta cgatgaaatt tttggccgta aagattacat ctctatcgat
    29041 tccccgatgg cccgcgcatt gctgaaaaaa gaagtcggcg atctggcggt ggtgaatacc
    29101 cctgccgggg aagcgagctg gtatgttaat gctatcgagt acgtgaaacc gtaaggaaga
    29161 gtcttaacct cctgccgatg gctggcattt ttgccagcca gtccgtataa ctatcccctg
    29221 atttttgatc cgaaaagatg aactcaaacc atgatgaatg attcgttctg ccgcattatt
    29281 gcgggtgaaa ttcaggcgcg cccggaacag gttgacgctg ccgttcgcct gcttgacgaa
    29341 gggaataccg tgccgtttat cgcacgttat cgtaaggaaa tcaccggcgg tctggatgac
    29401 acgcagctgc gtaatctgga aacgcgtctg ggatacctgc gcgaactgga agagagacgt
    29461 caggcgatcc tcaaatccat ttccgagcaa ggcaaactca ctgacgagct ggcgaatgcc
    29521 atcaacgcca ccttaagcaa aaccgaactc gaagacctct acctgcccta caaacctaaa
    29581 cgccgcaccc gcgggcaaat cgccattgaa gcagggcttg agccgttggc tgacctgctg
    29641 tggagcgatc cgtcacacac gccagaagtc gccgctgcac aatatgttga tgccgataaa
    29701 ggcgtggcag ataccaaagc cgcgctggac ggcgcgcgct atatcctgat ggaacggttt
    29761 gccgaagatg ccgcgctgct ggcaaaagtg cgtgattatc tgtggaagaa cgcgcatctg
    29821 gtctcaaccg tggtgagcgg taaagaagag gaaggggcaa aattccgcga ctatttcgat
    29881 catcacgaac cgttgtccac ggtgccttct caccgcgcgc tggcgatgtt ccgtgggcgt
    29941 aacgaaggcg tactccagct ttcgctgaat gccgatccgc agttcgatga gccgcccaaa
    30001 gagagctatt gcgagcaaat catcatggat caccttggcc tgcgcctgaa caatgccccg
    30061 gcggatagct ggcgcaaagg cgtagtgagc tggacctggc gcatcaaggt gctgatgcat
    30121 ctggaaaccg aactgatggg taccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc
    30181 tttgcccgta acctgcacga tctgctgatg gcggcccctg ccggactgcg tgcaacgatg
    30241 ggcctcgatc cgggtctgcg tactggggta aaagtggcgg tggtcgatgc cactggcaaa
    30301 ctggtagcga ccgataccat ttacccgcac accggacaag ccgcaaaagc agcgatgact
    30361 gttgctgcgc tgtgtgaaaa gcataacgtt gaactggtgg cgatcggcaa cggtacagct
    30421 tcccgcgaaa ccgaacgttt ctatctcgac gtgcagaagc agttcccgaa agtgaccgca
    30481 cagaaagtga tcgtcagcga agctggcgcg tcggtttact cggcttccga gctggcagcg
    30541 caggagtttc cggatctcga cgtttcgctg cgtggcgcgg tgtctatcgc ccgccgtttg
    30601 caggatccgc tggcggagct ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat
    30661 cagcatgacg tcagccagac gcaactggcc cgcaaactgg acgcagtagt agaagactgc
    30721 gtaaacgccg ttggcgtcga tctcaacact gcttctgttc cgctgttaac ccgcgtggcg
    30781 ggcctgacgc gcatgatggc gcaaaacatc gtggcctggc gcgatgagaa cggtcagttc
    30841 cagaaccgtc agcaactgct gaaagtgagc cgtctggggc cgaaagcctt cgagcagtgc
    30901 gcgggcttct tgcgcattaa ccatggtgat aacccgctgg acgcgtctac cgtccacccg
    30961 gaagcctatc cggtggtgga acgcattctg gcagcaacac agcaggcact gaaagatctg
    31021 atgggtaaca gcagcgaact gcgtaacctg aaagcgtctg actttactga tgaaaaattc
    31081 ggtgtgccga cagtaactga catcatcaaa gagctggaaa aaccgggtcg cgatccgcgt
    31141 ccggaattta aaaccgctca gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg
    31201 ggtatgatcc tcgaaggtgc ggtgaccaac gtcaccaact ttggcgcgtt tgtcgatatc
    31261 ggcgtgcatc aggacggcct ggttcacatc tcttcattgt cgaacaagtt tgtagaagat
    31321 ccgcataccg tggtgaaagc gggcgacatt gtgaaggtga aagtgctgga agtggatctt
    31381 cagcgtaaac gtatcgccct gaccatgcgt ctggacgagc agcctggcga aaccaacgcc
    31441 cgtcgcggcg gcggtaatga acgcccgcaa aacaaccgcc cggcagccaa accgcgcgga
    31501 cgtgaagcgc agcctgccgg taacagcgcg atgatggacg cgctggcggc agcaatgggc
    31561 aaaaaacgtt aaacgccctg attgacccct aaacctgcgc gataaggtgt ccacgccgca
    31621 tctggcaacg aacgccttgt ctgacataaa agtgccggag aatatctccg gcatttttat
    31681 tccacagcca aactcacaat atattccggc aacatttatc acttcattaa caactgaaac
    31741 cttaattaaa cattagccag tcctggtaat tcgctattcg aattatattt tcgctgcgat
    31801 ataaccttga gccacatcaa cattgagtca gattattatt caaaccaaca ttcgcacaca
    31861 ttttaagtat tgctgataga aaccattctc attatcattg tgttgttgat tatttaatct
    31921 ctcctttgtt ggcaaatcat ctggtctcat gtcgctgtca aacgccccat gaggtagtta
    31981 tccagttaat gagaaacaag taggcaccta tgcaatacac tccagatact gcgtggaaaa
    32041 tcactggctt ttcccgtgaa atcagcccgg catatcgcca aaaactgctt tctcttggca
    32101 tgttacctgg ctcctctttt aatgtggtgc gcgtcgctcc actcggcgac cccattcata
    32161 tcgaaacccg tcgtgtgagc ctggtattac gcaaaaaaga tctggcctta ttagaagtgg
    32221 aagcggtttc ctgttaatac ggtgataaca acaatgaaaa aattaaccat aggcttaatt
    32281 ggtaatccaa attctggcaa gacaacctta tttaaccagc tcactggcgc acgtcagcgt
    32341 gtaggtaact gggctggcgt taccgtcgaa cgtaaagaag ggcaattctc caccaccgat
    32401 catcaggtca cgctggtgga cctgcccggc acctattctc tgaccaccat ctcatcgcag
    32461 acctcgctcg atgagcaaat cgcctgtcac tacattttga gtggcgacgc cgacctgctg
    32521 attaacgtgg tggatgcgtc taaccttgag cgtaacctgt acctgacgct acaactgctg
    32581 gaactcggca ttccctgcat cgtggcactg aacatgctcg acattgccga gaagcaaaat
    32641 attcgtattg aaattgatgc tctgtcggcg cgtcttggct gcccagtgat cccgctggtt
    32701 tcaacccgtg gtcgcggtat tgaagcgctc aagctggcga ttgatcgcta taaagctaac
    32761 gagaatgtgg aactggtgca ttacgcacag ccgctactca acgaagcaga ttcactggca
    32821 aaagtgatgc cttccgacat cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg
    32881 gaaggcgata tctacagccg cgcctacgcc ggtgaagcgt cgcagcatct ggatgccgcc
    32941 ctcgcccgtc tgcgtaatga gatggacgat ccggcgctgc acattgccga tgcgcgttac
    33001 cagtgcattg ctgccatctg tgatgtggta agcaacaccc tgacggcaga acccagccgt
    33061 ttcaccactg cggtagataa aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc
    33121 tttgtgatgt acctgatgtt cctgctggct atcaacatcg gcggggcgtt acagccgctg
    33181 tttgacgtcg gctccgtggc gctatttgtg catggtattc aatggattgg ctacacgctc
    33241 cacttcccgg actggctgac tatcttcctc gcccagggcc tgggtggcgg cattaacacc
    33301 gtgctgccac tggtgccgca gattggcatg atgtacctgt tcctctcctt ccttgaggac
    33361 tccgggtata tggcgcgtgc ggcgtttgtg atggaccgtc tgatgcaggc gctgggcttg
    33421 ccggggaaat cctttgtgcc gctgatcgtc ggtttcggtt gtaacgtacc gtcggtaatg
    33481 ggtgcacgta cgcttgatgc accgcgtgaa cgtctgatga ccatcatgat ggcaccgttt
    33541 atgtcctgcg gcgcgcgtct ggctatcttc gcagtattcg cggctgcctt cttcgggcag
    33601 aacggtgcgc tggcggtctt ctcgctgtat atgctgggta ttgtgatggc ggtgctgact
    33661 ggcctgatgc tcaagtacac catcatgcgc ggtgaagcga cgccgtttgt catggagttg
    33721 ccggtctatc atgtaccaca cgttaaaagc ctgattatcc agacctggca gcgtctgaaa
    33781 ggcttcgttc tgcgtgctgg taaagtgatc atcatcgtca gcattttcct gagcgctttc
    33841 aacagcttct cgctgagcgg gaaaatcgtc gataacatca acgactcggc gctggcgtcc
    33901 gtcagccggg tgatcacccc ggtcttcaag ccaattggcg tgcatgaaga taactggcag
    33961 gcaacggttg gcctgtttac aggtgccatg gcgaaagaag tggtagtggg tacgctcaac
    34021 accctctaca ccgcagaaaa tattcaggac gaagagttca atccggcaga gtttaacctc
    34081 ggtgaagagc tgttcagtgc gatagatgaa acctggcaga gcctgaaaga caccttcagc
    34141 cttagcgtac tgatgaaccc cattgaagcc agcaaaggcg acggcgaaat gggtaccggg
    34201 gcgatgggcg tgatggatca gaaattcggt agcgcagcag cagcttacag ctacctgatt
    34261 ttcgtcctgc tgtatgtacc atgtatctcg gtgatgggag ccatcgcccg tgaatcaagc
    34321 cgtggctgga tgggcttctc catcctgtgg gggctgaata tcgcttactc gctggcaaca
    34381 ttgttctatc aggtcgccag ctacagtcag cacccaactt acagcctggt gtgcattctg
    34441 gcggttatcc tgtttaacat cgtggttatc ggtctgctgc gccgcgcgcg tagccgggtg
    34501 gatatcgaac tgctggcaac ccgcaagtcg gtaagcagtt gctgcgcagc cagcaccacc
    34561 ggtgattgcc attaatggct tcacttattc aggtgcgcga tttgctggcg ttacggggcc
    34621 gtatggaagc ggcccagata agccagacat tgaacactcc acagccaatg attaacgcca
    34681 tgctgcaaca actggaaagt atgggcaaag ccgtgcggat tcaggaagaa cctgacggct
    34741 gcctctctgg cagttgtaaa agctgcccgg aaggaaaagc ctgtctgcgc gagtggtggg
    34801 cgctgcgtta accttactcc atcgcctgtt ttggaaagcc cggtatgcgt ctgcatccgg
    34861 gcttttttgc gtgcggcttt ccataaaaat gcaactcttg cagcacggcg taagttcctt
    34921 tgaaagcatc tcgcagggat gaaaactcgc taatgcacag atgtggagtg gcgcgtagag
    34981 tcgcggcatt caaacaacag gtgaaggaac gccatgagta aaaagcagag ttccacccca
    35041 catgatgcgc tgttcaaact ctttttacgc caaccggaca cggctcgcga ttttcttgcg
    35101 ttccatttac cggcccccat tcacgcgctc tgtgatatga aaaccctcaa gctggagtcg
    35161 agcagcttta ttgatgacga tctgcgtgaa agctattccg atgtgctgtg gtcggtgaaa
    35221 acggaacaag gaccaggata catctattgt ctgattgaac atcaaagcac ctcaaacaaa
    35281 ctgatcgcat ttcgcatgat gcgttacgct attgccgcaa tgcaaaatca ccttgatgct
    35341 ggatacaaaa cgttgccgat ggtggtgcta ttgttgtttt accacggtat tgaaagcccc
    35401 tatccctatt cgctgtgttg gctggattgt ttcgccgatc ccaaactggc aaggcagctt
    35461 tatgcctccg catttccgct gattgatgtc accgtcatgc ctgatgatga aatcatgcag
    35521 caccgacgta tggcgctgct ggagttaatt caaaaacata ttcgtcaacg cgacctgatg
    35581 gggctggtag agcaaatggc ctgcttatta agtagtggat acgctaatga cagacaaatc
    35641 aaagggctgt ttaattacat actgcaaacc ggcgacgcgg tacgttttaa cgattttatc
    35701 gacggcgttg ccgaacgttc accgaaacac aaggagagtt taatgacaat tgcggaaaga
    35761 ttgcggcagg agggggaaca atccaaagcc ctgcatatag ccaaaataat gcttgaatcc
    35821 ggagtccctc ttacagacat catgcgcttt accggggtgt cagaagaaga gttggctgcg
    35881 gcgagccagt aaatttctgt ctcgccattt caaaagttgc ctacaccctc tgcttcaacg
    35941 ccaccagcag gtgacaaaac tcgaccggat gcgaaataaa cggcgcatgg gccgctttgg
    36001 cgaagatata tgattcgctg tgaggccaaa gtttatccag catcggcacc actttgcgcg
    36061 gcaccagacc gtcgagatag ccatacaatc gcaaaaacgg catgggcacg ttttgcagcg
    36121 gctgacggag atcgaccgtt ttcaggattt ccagcccgcc attaagcacg tcaacctccg
    36181 gcatcggtaa cgccagaacg gttttcttca acgcccgcgc atcctggcgc gccgtttcag
    36241 tgcccatggt ttgtaacgcc aggaaccgct ccactgtacg ctgaaaatca tcactgagtt
    36301 gctgctgaaa tcccgccagc acgtccggtt ttatccccgg ccactcgtca cgagcactaa
    36361 aacaaggtga cgacgccacg gtgaccagcg cctgaacacg ctcgggatgg gttaacgcaa
    36421 tctggcttgc caccagcccg cccagactcc agcctaacca aatggcttta tcaggtgcct
    36481 gttgcagcac ggcttcggcc atatcagcaa gtgacagcgc accaaatccc cggctacgcc
    36541 cgaagccggg caggtcaaca aggtgcagcg taaaatgcga gctaagttcc tcgtcaatgc
    36601 aacgccacac ttcggcattc agtccccatc cgtgcagcag cacaagatga acattcccct
    36661 gacctttggt ctgccaccag atgttattca tccgctattg ttctcttttg acttacaagg
    36721 atgaacatat gctaacagta ccgggattat gctggctatg ccgaatgcca ctggcgttag
    36781 gtcattgggg gatttgttcg gtctgttcac gcgccacccg cacagataaa acgttatgcc
    36841 cacaatgtgg attacccgcc acacactccc atcttccctg cggtcgctgc ctgcaaaaac
    36901 cgccgccctg gcaaagactg gtcacagttg ccgactatgc gccgccgtta agtctgctca
    36961 tccaccagct taaattttcc cggcgcagtg aaatcgccag cgccctgtca cgtctgttac
    37021 tattggaagt cttgcacgct cgtcgcacca ccgggttgca attgccggat cgcatcatca
    37081 gcgttccgtt atggcagcgg cgtcactggc gtcggggatt taatcagagc gatttgctgt
    37141 gtcagccgtt atcacgctgg ttgcactgcc aatgggatag cgaagccgtc acacgtacac
    37201 gggccactgc gacccagcat tttctcagtg cccggctgcg caagcgcaac ctgaaaaatg
    37261 cctttcgtct tgaattgccc gtgcaaggtc gccatatggt gattgtggat gatgtcgtta
    37321 ccaccggaag taccgtcgca gagattgcgc agttgctttt acgcaatggt gcggcgactg
    37381 tccaggtctg gtgcctttgt cgaaccttgt agagcctcga tgatgggcgt attataacca
    37441 actaaaatag tcaactatta ggccattact atgatccgta tttccgatgc tgcacaagcg
    37501 cactttgcca aactgctggc aaatcaggaa gaagggacac aaatccgcgt atttgtgatt
    37561 aaccctggca cgcctaacgc tgaatgtggt gtttcttatt gtccgccgga tgccgtggaa
    37621 gccaccgaca ctgccctgaa atttgacctg ctgaccgcgt atgttgatga gttaagcgca
    37681 ccatacctgg aagatgcaga gatcgacttt gttaccgacc agttgggttc tcagttaacg
    37741 ctgaaagccc cgaacgccaa aatgcgtaaa gtggcagacg atgcaccgct gatggagcgc
    37801 gtggagtata tgctgcagtc gcagatcaac ccacagcttg ctggtcacgg tggtcgcgtt
    37861 tcgctgatgg aaatcacaga agacggttac gccattctgc aatttggcgg cggctgtaac
    37921 ggttgttcca tggtcgatgt gacgctgaaa gaagggatcg agaagcagct gctgaacgaa
    37981 ttcccggagc tgaaaggtgt gcgcgatctc accgaacacc agcgcggcga acactcctac
    38041 tactaagtta tcccctcttt tgtagattgc ccgatgcgac gctaaagcgt catttcgggc
    38101 ctacgttgaa catgtgccag acgttggcac agcattgctt tggcaaatcc ctcccttcgt
    38161 ttccccgcga taatatgacc aacctctcat aatttaaatt taccccgctc tggtgattct
    38221 caaacgccag atgttacccg tatcattcac atgggtacca aacatactcc tgacatctga
    38281 ctacaataat tagttttagt gggtatcagt cgtggtgccg caatatctct gttcccgatt
    38341 gggataatta gagtttgtcg tcagaaaatt gacgttaccc ataacaaatg aaaggccagg
    38401 taaatcatgc cattagtcat tgttgctatc ggtgtaatct tgttgttgct cctgatgatc
    38461 cgcttcaaaa tgaacggctt catcgctctc gtcctcgtgg cgcttgctgt tggattaatg
    38521 caaggaatgc cgctggataa agttattggc tccatcaaag ccggtgtcgg cgggacgctc
    38581 ggtagccttg ccctgatcat gggttttggc gcaatgctgg gcaaaatgct ggcagactgc
    38641 ggtggcgcac aacgtatcgc caccacgctg attgccaaat ttggtaaaaa gcacatccag
    38701 tgggcggtgg tgttaaccgg ttttaccgtt ggttttgccc tgttctatga agtgggcttc
    38761 gtgctgatgc tgcctctggt gtttaccatc gcggcttccg cgaatattcc actgctgtat
    38821 gttggtgtac caatggcggc tgcactgtct gtgacccacg gcttcctgcc accgcatccg
    38881 ggcccgactg cgattgccac cattttcaat gccgatatgg gtaaaaccct gctgtacggt
    38941 actattctgg caatcccgac cgtgattctc gccggtccgg tttacgctcg cgtgttgaaa
    39001 ggtatcgata agccaatccc ggaaggtctc tacagcgcga aaaccttcag cgaagaagag
    39061 atgccgagct ttggcgtcag cgtctggact tctctggtgc cggttgtgct gatggcgatg
    39121 cgtgcgattg ccgaaatgat cctgccgaaa ggtcacgctt tcctgccggt agcggagttc
    39181 ctcggtgacc cggtaatggc aacgctgatt gccgtgctga ttgcgatgtt cacctttggt
    39241 ctgaaccgtg gtcgttcaat ggatcagatt aacgacacgc tggtttcttc catcaaaatc
    39301 attgcgatga tgctgttgat catcggtggt ggcggtgcgt tcaagcaggt gctggtagac
    39361 agcggcgtgg acaaatacat tgcttccatg atgcacgaaa ccaacatttc tccgctgctg
    39421 atggcctggt cgattgctgc cgtactgcgt atcgcgctgg gttccgcaac cgttgcggca
    39481 atcactgcgg gtggtatcgc ggcaccgctg attgcaacga cgggtgttag cccagagctg
    39541 atggttattg cggtgggttc cggtagtgtg attttctctc atgtgaacga tccgggcttc
    39601 tggctgttca aagagtactt taacctgact atcggcgaga ccatcaaatc ctggtcgatg
    39661 ctggaaacca ttatctcggt gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt
    39721 tgataacact gactgccgga tgcatcgcat ccggcatcaa ccgcactcta cttcttcttc
    39781 gctgcagctc tgcgccgtct gtccaaatcc ttcagcaact tgttcacgcc atcatcggca
    39841 aacatcgtct caagcgttgc ggaaagcttg cgtcgccagt ttttatactg gtaactggtg
    39901 ccaggaatat tcaccggttc ggccatatcc agccagtctt ccggctgtag tcctaacaga
    39961 gcactgttac tgtcggcaat gtagcgctgc aaaccacggt tcagcgtcgg cgtcatcgac
    40021 atcaacgatg ccttatgccc ggcacgtttt ggcagacaac catatttatg cagtgcatcc
    40081 agcagccctt gcttcgccag ttcgcgatcc tgatacagac cgcgcagtac cacttcatcc
    40141 ggatacagcc ccagggtttt gcccagcgtt agatccccgc tctcccagta accgcgtagc
    40201 gtaggcaggt catgtgtcgc cgcaaccgcc atcgactgct ccggatacgc tttcggtgca
    40261 cggaacgtct tctcgtggtc gttttcgaaa tagagcactt tgtaggagta cacaccgctg
    40321 ctgcgcagct taccgacaat ctctaccggt acggtaccga gatcttcacc aatcaccata
    40381 cagcgatgac gtttactttc gagtgccaga atcgagagca gatcatccac cggatagtga
    40441 acatacgcgc cctgatctgc cgtctcgcca tacggtatcc accacaaacg cagcatcgac
    40501 atcacatggt caattcgtaa tgcgccgcag ttttgcatat tggcacgcaa cagctcgata
    40561 aacggttcat aggcacgcgc ggtgatgata tgcgggtcca ttggcggtaa tccccagttc
    40621 tgccccaacg ggccgaggat atccggcggc gcgccaaccg acgctttcag gcaatatagt
    40681 tcacggtcac accaggtttc cgccccacct tccgctacgc caaccgccag atcacgatac
    40741 aagccaatcg gcatttcata gccctggctt atctcccagc aggcggcaaa ctggctgtaa
    40801 gccagccact gcaaccagag ataaaaatcg acgtcattac gatgttcttc gcagaactga
    40861 cgcacttctg gtgaatccac gttctgatac atctctggcc atgcaggcca gccccagcgc
    40921 atttcgtcct ctttcacttg ctgggcatgt agcgcatcaa aggctgcctg ccagaacagg
    40981 ctgtcgccct gctctgcaac aaactggcga aacgcggtca tctgctcatc atcacgttgc
    41041 gcgaaacctt tccacgccat tcgtaatgct gtcattttta gggcggtaac cgtggagtaa
    41101 tcgacccagt cggcatcgcg cgcctgttgc agcgtctgtt gcgtggtcgg caactgccac
    41161 caggcctgag cctcttcgct aagatggaaa tcttcaacgg cgttaacgtc gatataaatc
    41221 acattcagcc aacggcgaga agacgggctg tatgggctgg cactctccgg atttgccgga
    41281 tagagcgcat gaatcgggtt caggccgatg aacgacccgc cacgttttgc cacatccacc
    41341 agcatcgctt tgagatcgcc aaaatcccca ataccccagt ttttttccga tcgcagcgta
    41401 taaagctgaa cgcaggcacc ccacagcttt tgtttattca gcaacgcctg cggttcgtaa
    41461 cagcgtttcg gggcgacaat cacccggcaa tgcgcgcgct ggtcgtcctg ggtgagtgtc
    41521 agcgtgtgat aaccttccgg cagcttcgtc ggtagattga acgctttgcc ccccgttaca
    41581 tggcctttgt actgtgttcc ttcttcggtg gtcagcagcc agctatattc gccgctgccc
    41641 tccaccacca tcggcatttt tttgccgctg gtataaacca tgacattcgg gactggcgtt
    41701 accgccactt tcgtggcggt acgttgatgc atcgcgtcaa gcaaacgccg tttggtttcg
    41761 gcgctaatcg actgcggttt accgtgggca ttgatgtaat tggggctaat ccccgccgcc
    41821 agcgcggcat tatccagacg tttgctttcc atcgagcttc cttagcgttt tgcctgccag
    41881 atacgagcct gataatcgcg aatagagcga tccgagctaa acataccgca gcgggcggta
    41941 ttgaggatcg ccgcgcgagt ccaggcctcc tggtcgcggt acagcacatc cacctgcttt
    42001 tgtgcctcaa cataggctgc gaaatccgcc atcaccagat acggatcgcc gccttgtttg
    42061 ccgatactgt gcagcatctg gtcgaaggca tgcttatcgc cgtcgctgta tttaccgctt
    42121 tccagctctt tcaatactgc gtccagcacc ttatctttct tccgccattt caccgggtcg
    42181 tagcctttgg ccagaatagc cttcacttgt tccacggtat gaccaaaaat aaagatattt
    42241 tcttcaccga ctttctcggc gatttcaacg ttcgcaccat ccagcgtacc gacagtaagc
    42301 gcaccattga gcgccagttt catattgccg gtaccggaag cttctttacc tgcagtcgaa
    42361 atttgttcgg agatatccgc cgccgggatc agtttttccg ccgccgaaac gcaataatcc
    42421 ggcaggaaca ccaccttcaa cttatcgcca accagtggat cgttgttgat cacgtcagcc
    42481 actttgttga tcgcaaagat aatattcttc gccaggtagt agcccggtgc cgctttcgcg
    42541 ccgaagagga agacgcgcgg tacgcgatca gcctgcgggt tttcacgaat ttctttgtac
    42601 aacgccagaa tatgcagcag attcaggtgc tggcgtttgt actcatgcag acgtttgatc
    42661 tgaatatcga aaatcgcctg tggattgatc tcaataccgg tacgaacttt cacaaactcc
    42721 gccagacgga ctttattcgc ctgcttgatc tcgcgatatt gctgacggaa tttcgcatca
    42781 tcagcgaatt tttccagatt gatcagctga tcgagatcgt tagcccactc tttttgcagt
    42841 gatttatcca acagagccgc cagtgcaggg ttgcactgtt tgatccagcg acgtggggta
    42901 ataccgttgg tgacgttatg gaatttgttc ggccatagct ggtgatattc cgggaacaga
    42961 tctttcacca ccagatccga gtgcagcgcc gcaacaccgt tcaccgcgaa accgccaacc
    43021 acacacaggt tcgccatatg cacttgttta tcgtgcacca ccgccagttt ggcccacact
    43081 ttttcatcgc ccggccaggt tttctccacc agcgttttaa agcgagtatt aatttcgtta
    43141 ataatctgca tatggcgcgg cagtaagcct ttcaccagtt tcacatccca gcgttccagc
    43201 gcttctggca tcagggtatg gttggtgtaa gcgaaagttt tgctggtaat ggcccaggcg
    43261 tcatcccagc tcatctggtg ctcatcgatc agcacgcgca gcagttctgg aatcgcgata
    43321 gttgggtggg tatcgttcag ctgaataact tcgtaatccg ccagttcgtg cagtttacgc
    43381 cccgccagat gatggcgacg caaaatatcc gctaccgaac aggcacactg gaagtactgc
    43441 tgcatcaagc gcagcttttt accggcagta tggttgtcgt ttggatagag aactttggtc
    43501 agtttttccg cattga
//
LOCUS       DAFATA010000024        43204 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFATA010000024 DAFATA010000000
VERSION     DAFATA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.024"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..460
                     /locus_tag="LGJ20_001791"
     CDS             <1..460
                     /locus_tag="LGJ20_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385860.1"
                     /translation="RVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQVLPLF
                     LLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIVHIKGM
                     ASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            509..1096
                     /locus_tag="LGJ20_001792"
     CDS             509..1096
                     /locus_tag="LGJ20_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBK2385861.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(1158..1880)
                     /locus_tag="LGJ20_001793"
     CDS             complement(1158..1880)
                     /locus_tag="LGJ20_001793"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK2385862.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(1895..3064)
                     /locus_tag="LGJ20_001794"
     CDS             complement(1895..3064)
                     /locus_tag="LGJ20_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK2385863.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGGPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDECVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(3091..4707)
                     /gene="bglH"
                     /locus_tag="LGJ20_001795"
     CDS             complement(3091..4707)
                     /gene="bglH"
                     /locus_tag="LGJ20_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBK2385864.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(4793..6187)
                     /gene="bglB"
                     /locus_tag="LGJ20_001796"
     CDS             complement(4793..6187)
                     /gene="bglB"
                     /locus_tag="LGJ20_001796"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBK2385865.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(6206..8083)
                     /gene="bglF"
                     /locus_tag="LGJ20_001797"
     CDS             complement(6206..8083)
                     /gene="bglF"
                     /locus_tag="LGJ20_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBK2385866.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAAKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(8216..9052)
                     /gene="bglG"
                     /locus_tag="LGJ20_001798"
     CDS             complement(8216..9052)
                     /gene="bglG"
                     /locus_tag="LGJ20_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBK2385867.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(9338..10063)
                     /gene="phoU"
                     /locus_tag="LGJ20_001799"
     CDS             complement(9338..10063)
                     /gene="phoU"
                     /locus_tag="LGJ20_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK2385868.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(10078..10851)
                     /gene="pstB"
                     /locus_tag="LGJ20_001800"
     CDS             complement(10078..10851)
                     /gene="pstB"
                     /locus_tag="LGJ20_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBK2385869.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10942..11832)
                     /gene="pstA"
                     /locus_tag="LGJ20_001801"
     CDS             complement(10942..11832)
                     /gene="pstA"
                     /locus_tag="LGJ20_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK2385870.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11832..12791)
                     /gene="pstC"
                     /locus_tag="LGJ20_001802"
     CDS             complement(11832..12791)
                     /gene="pstC"
                     /locus_tag="LGJ20_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBK2385871.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12878..13918)
                     /gene="pstS"
                     /locus_tag="LGJ20_001803"
     CDS             complement(12878..13918)
                     /gene="pstS"
                     /locus_tag="LGJ20_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK2385872.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(14166..15239)
                     /locus_tag="LGJ20_001804"
     CDS             complement(14166..15239)
                     /locus_tag="LGJ20_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2385873.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(15250..17772)
                     /locus_tag="LGJ20_001805"
     CDS             complement(15250..17772)
                     /locus_tag="LGJ20_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2385874.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(17797..18528)
                     /locus_tag="LGJ20_001806"
     CDS             complement(17797..18528)
                     /locus_tag="LGJ20_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBK2385875.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(18576..19148)
                     /locus_tag="LGJ20_001807"
     CDS             complement(18576..19148)
                     /locus_tag="LGJ20_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBK2385876.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            complement(19450..21279)
                     /gene="glmS"
                     /locus_tag="LGJ20_001808"
     CDS             complement(19450..21279)
                     /gene="glmS"
                     /locus_tag="LGJ20_001808"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK2385877.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(21441..22811)
                     /gene="glmU"
                     /locus_tag="LGJ20_001809"
     CDS             complement(21441..22811)
                     /gene="glmU"
                     /locus_tag="LGJ20_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK2385878.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(23162..23581)
                     /gene="atpC"
                     /locus_tag="LGJ20_001810"
     CDS             complement(23162..23581)
                     /gene="atpC"
                     /locus_tag="LGJ20_001810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK2385879.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(23602..24984)
                     /gene="atpD"
                     /locus_tag="LGJ20_001811"
     CDS             complement(23602..24984)
                     /gene="atpD"
                     /locus_tag="LGJ20_001811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK2385880.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(25011..25874)
                     /gene="atpG"
                     /locus_tag="LGJ20_001812"
     CDS             complement(25011..25874)
                     /gene="atpG"
                     /locus_tag="LGJ20_001812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK2385881.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(25925..27466)
                     /gene="atpA"
                     /locus_tag="LGJ20_001813"
     CDS             complement(25925..27466)
                     /gene="atpA"
                     /locus_tag="LGJ20_001813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK2385882.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(27479..28012)
                     /gene="atpH"
                     /locus_tag="LGJ20_001814"
     CDS             complement(27479..28012)
                     /gene="atpH"
                     /locus_tag="LGJ20_001814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK2385883.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(28027..28497)
                     /gene="atpF"
                     /locus_tag="LGJ20_001815"
     CDS             complement(28027..28497)
                     /gene="atpF"
                     /locus_tag="LGJ20_001815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK2385884.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(28559..28798)
                     /gene="atpE"
                     /locus_tag="LGJ20_001816"
     CDS             complement(28559..28798)
                     /gene="atpE"
                     /locus_tag="LGJ20_001816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBK2385885.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(28845..29660)
                     /gene="atpB"
                     /locus_tag="LGJ20_001817"
     CDS             complement(28845..29660)
                     /gene="atpB"
                     /locus_tag="LGJ20_001817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK2385886.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(29669..30049)
                     /gene="atpI"
                     /locus_tag="LGJ20_001818"
     CDS             complement(29669..30049)
                     /gene="atpI"
                     /locus_tag="LGJ20_001818"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBK2385887.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(30666..31289)
                     /gene="rsmG"
                     /locus_tag="LGJ20_001819"
     CDS             complement(30666..31289)
                     /gene="rsmG"
                     /locus_tag="LGJ20_001819"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK2385888.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(31353..33242)
                     /gene="mnmG"
                     /locus_tag="LGJ20_001820"
     CDS             complement(31353..33242)
                     /gene="mnmG"
                     /locus_tag="LGJ20_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK2385889.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(33621..34064)
                     /gene="mioC"
                     /locus_tag="LGJ20_001821"
     CDS             complement(33621..34064)
                     /gene="mioC"
                     /locus_tag="LGJ20_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBK2385890.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(34154..34612)
                     /gene="asnC"
                     /locus_tag="LGJ20_001822"
     CDS             complement(34154..34612)
                     /gene="asnC"
                     /locus_tag="LGJ20_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBK2385891.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            34764..35756
                     /gene="asnA"
                     /locus_tag="LGJ20_001823"
     CDS             34764..35756
                     /gene="asnA"
                     /locus_tag="LGJ20_001823"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBK2385892.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(35761..37212)
                     /gene="viaA"
                     /locus_tag="LGJ20_001824"
     CDS             complement(35761..37212)
                     /gene="viaA"
                     /locus_tag="LGJ20_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBK2385893.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(37206..38702)
                     /gene="ravA"
                     /locus_tag="LGJ20_001825"
     CDS             complement(37206..38702)
                     /gene="ravA"
                     /locus_tag="LGJ20_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBK2385894.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            38925..40793
                     /gene="kup"
                     /locus_tag="LGJ20_001826"
     CDS             38925..40793
                     /gene="kup"
                     /locus_tag="LGJ20_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBK2385895.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            40960..41379
                     /gene="rbsD"
                     /locus_tag="LGJ20_001827"
     CDS             40960..41379
                     /gene="rbsD"
                     /locus_tag="LGJ20_001827"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK2385896.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            41387..42892
                     /gene="rbsA"
                     /locus_tag="LGJ20_001828"
     CDS             41387..42892
                     /gene="rbsA"
                     /locus_tag="LGJ20_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBK2385897.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            42897..>43204
                     /gene="rbsC"
                     /locus_tag="LGJ20_001829"
     CDS             42897..>43204
                     /gene="rbsC"
                     /locus_tag="LGJ20_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBK2385898.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     A"
BASE COUNT        10700 a        11023 c        10404 g        11077 t
ORIGIN      
        1 tcgggtaata catcacggac tttattttgc ggttttagga ccgttaattg gtgttctgtt
       61 tcttgtcctc tacatattct tcgcaaaaga accgctggtt cttttggtaa taatacaggt
      121 acttccattg tttctcttat tgtcgattac tacgggagct attcctgcat tgttaaccgg
      181 cgtgatggtc gcctgtctgc cggaaaagat cgggtcacag aaaaattatc gttgtctggc
      241 tggtggcata ggtggcgtcg ttatcaccga gatctattgt gcagttattg tacatattaa
      301 gggcatggct tcctcggagt tgtttgaaaa cattctttct ggtgacagtc tcgttgtccg
      361 catcattcct gcattgctgg caggtgtggt gatgagcaga atcattaccc gtctgcccgg
      421 attggatatt tcatgtcctg aaacagactc tttaagttaa gcgggatact ttatctttgg
      481 gctactcaaa agcagacagg atgttactat gactcaaaat atcaggccgt taccccaatt
      541 caaatatcat cccaagccac tggaaacagg cgcatttgaa caggataaaa ccgtagagtg
      601 cgattgctgt gaacaacaga cgtcagttta ttactcgggt cccttttatt gcgttgatga
      661 agttgaacat ctctgtccgt ggtgtattgc ggacggttct gctgctgaaa agtttgcagg
      721 tagttttcag gatgatgcca gcatagaagg tgttgaattt gagtatgatg aagaggacga
      781 atttgccggt attaagaaca catatcctga tgaaatgctg aaagagttgg ttgaacgcac
      841 gccaggttat catggatggc aacaggaatt ctggcttgcg cattgtggcg atttctgtgc
      901 ttttatcggc tatgtgggct ggaatgatat aaaagatcgc ctcgatgaat ttgccaacct
      961 tgaagaagat tgtgagaatt tcggtattag aaattctgat ctagctaaat gcctgcaaaa
     1021 gcgtggtgat tgtcagggtt atctcttccg ctgtctccac tgcggcaagc tgagactgtg
     1081 gggtgacttt tcgtagttat ttaaataatg agaacaggcc ggagcgtaat tcatgcatcc
     1141 ggccttattt cttcagctta accgagcgct aattctgcgg cagcggcttt atcagcgatt
     1201 accatcagcg aaggatgcag ctgcaacacg gaagctggaa catcttcggt aaccggacct
     1261 tgcagcacat ttttcagtgc ctgcgcttta cctgcgccac tgacaattat cagcaagttt
     1321 ttcgccgcca tgatgctttt cggccccatc gtcacgtagc tgtcaggcac taacgaaaag
     1381 tcgccgccca actcaccatg cgccacaata tcaaccatct ctccctggat cgggaactcc
     1441 acggtttgct catggaaatg ggtcgtattc ggcaaattgc cacaaaaatg accatctgca
     1501 cctaatccca gcaccaccaa atccaggccg ccttcacgcg ccagtttctg atcatgctcg
     1561 cggtagttat caatagtgag cttctggata ttctcttctt tgatccccgc aggggtgaaa
     1621 aacagattac gcagattggt aatcgttacg ccttctccct ctttgccacg aaagggaatt
     1681 tcatcgaaat tatagaaata gcagttatcg taccagggct tacctttaac cagggtggtg
     1741 aggtattcat acatgccttt gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc
     1801 gtcttcgaca tataaccaag cagatggtgt gccgcgacac ggctcatttc ctggtaatct
     1861 tcggtaatga ttaatttcat cttaatgcct tattttattt aaataatttc tgtacgaagg
     1921 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
     1981 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
     2041 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
     2101 gatcgttcag ccgggccgcg acacattcat ccggtacggt tgtcgtggta acaccactga
     2161 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc
     2221 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ttaaaacgct
     2281 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagcg
     2341 gataaaagac tttacgtctt tcctgaggaa cgaaatcttc gggaataatg cccttcgcat
     2401 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
     2461 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccggta tcaccaaaac
     2521 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
     2581 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
     2641 caccatgcgc aacagaacgc gtatccagat aactgcctgg caccagaatc atactggagt
     2701 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
     2761 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
     2821 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaccaccc accactacag
     2881 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
     2941 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
     3001 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
     3061 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
     3121 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
     3181 tttgatatac gtggcgtaga aacgaatttc cggacgcgag gtcaacatac tggtattgac
     3241 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
     3301 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
     3361 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
     3421 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
     3481 gccgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
     3541 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttact
     3601 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
     3661 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatagc cgttattatc
     3721 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
     3781 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat tggtattaat
     3841 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
     3901 atcaatttta cccggaccga ctttccagtt ttccagacct acacccgctg cggcatcagt
     3961 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
     4021 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
     4081 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
     4141 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
     4201 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
     4261 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
     4321 gccgttgtaa ctaaagccaa tttcatcttt cacaaactta ctgaaatctt tcagagtcat
     4381 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
     4441 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
     4501 acttacgcta cgattcaccg tcgctggcgt atattttttc ttctcttcat ctttatactt
     4561 tttgagttcg ctttgcgttt ctcttaacgc tttttctaat aactcaaggc gatgttccac
     4621 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagatggc
     4681 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
     4741 gtatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttatt ggttattttt
     4801 ttaatgacag cccccgcgtt ttgatcactt ctgcatacca tccgaagctt ttcttacgcg
     4861 ttcttgtgag gcttccttcg ccattatcat cacgatccac ataaataaag ccgtagcgct
     4921 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
     4981 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatcg tttaaatagg
     5041 caattcgata atcgtcctgt atcgaaccat ccgcttcaac gctgtctttt gcgcctaatc
     5101 cgttctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaaccc
     5161 gtaatccaac cgaatcaatt tgccaccccc actctgaact tttcagatgc ggattgggga
     5221 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
     5281 cagtcatgta ataactaaaa gagatgaaat cgacggtatg ttttaaatct tctgcgtcac
     5341 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
     5401 actggccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
     5461 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
     5521 cgattttcgc ttcggggagc aggctatgac aggctttaac tgcccgcgca ctggcaacca
     5581 gttgatggtg gatagcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
     5641 ccgtgaatgg ctcgtgtaac gacatgttga tttcattaaa cgtcagccat aacgccactt
     5701 tatgttggta gcgagtaaag accgtgcggg cgtaatgctc gaagtgatcg atgaccgctc
     5761 gattagccca accgccgtag tttttcacca gcccatatgg catttcgtaa tgggataacg
     5821 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta
     5881 accccgcttc attcggttcg acttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
     5941 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
     6001 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
     6061 ttcccattac gccatgaggc tgtaaatctg aggtcgagat ccctttgcca tcttcctgcc
     6121 aggcaccttc cacctgattg gcagctgttg cgccacccca aagaaatgtt tctggaaatg
     6181 ctttcataat taacttcttt tatcgttagc gaatgatgga taacagcggt tcacctgcgc
     6241 ttatctgcgc cgtgccgtgg ggtaatacgt ccgtaaaatc atcgctatta ctgattaata
     6301 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
     6361 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
     6421 gttttacggt gtcgataccg acatgaatca ggagctccac accatcatct gactcaatgc
     6481 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgcaaccgga gaacgcactt
     6541 caccaaccga gggcagaatg gcgatacctt tacccaacag gccactggca aacgtggtat
     6601 cagcgacgtg aatgagcggc acaatctctc ccgtcatcgg tgaacagata ccgccctgct
     6661 caggtggtgt aataacctct ggtgttttct cttgcggggc accctgcgct ggctgacgtt
     6721 tagcggtgat gaaatgaagc atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac
     6781 cgccaataac gctggcccag acggtgaaat caattcccgt tgacgggatg gtttgcatga
     6841 aggtgaaaat acttggcaaa ccaaaggagt acactttcgt ttgcgcgtag ccaatgatgg
     6901 tggcccccaa agccccactg atacaggcga taacaaaggg gtacttacgc ggcagattaa
     6961 cgccatatac cgctggttcg gtgataccaa acaggcccgt caacgccgct gatcccgcca
     7021 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg
     7081 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
     7141 tgatacacag cggcaccagg ccccagtgca gtccgaacat gacgaagatt tgccagaagc
     7201 cgcccattac cgcgcctgca aatgcaggaa ccgcctgata aagccagaga taaccggcgg
     7261 caatcagttc gcttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga
     7321 taaccatcag acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat
     7381 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
     7441 acgagtaatt caacaatgtg accggaatac ccaggaaatc cagccccagc tcatccgctt
     7501 tttgcccgtt ctcgaaagca gtcagaatta atggatgcac taacgctcca ccaatcacca
     7561 tggcagtaaa tggattaccg ccgaagcgtt tcgccgcggt gtatcccagg attatcggga
     7621 agaaccaaaa caaggcatca ctggcgctga ataaaattaa ataagtacca ctttgttcgg
     7681 tcgtccactg aaaagtgagc gccagagcca gcataccttt caggatcccg gttgccgcca
     7741 tcaaaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttagcagat
     7801 tacctttatc atcattttcc ggtgcctgtt gcgctttttc gtcaaggcct gccacactgt
     7861 taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac tggccaccgc
     7921 tttccaccac cataataata ccgggggtct ttttcagtac ctctgcttgc gctttgcttt
     7981 catcctttaa cttaaaacgt aatcgcgttg cgcaatgcat cagactcaca atgttatctg
     8041 cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc cctctaccgc
     8101 tttgcggcaa aactcaaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
     8161 ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata atatttcagt
     8221 gttctttgcg cacgcgctct atatttatgg ctaaaaacat aatctctgcg ggtgagattt
     8281 tacgttgata ctgcaaacca ataaaaatgg cgatccgctc cgcacattgc catgcttgcg
     8341 ggtaattttg ttttactgct tgttgtaatg actcatcact atcgttaatt gaagcatgtc
     8401 caagaatacg ccaggataaa aacttcagat gcgtaaccag tcgctgataa ctcaagcttt
     8461 cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg cgcattaatt
     8521 gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc agatgcatgg
     8581 caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca ataatggtta
     8641 acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc cacagcagcg
     8701 ggttgggcag taacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
     8761 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataatac
     8821 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
     8881 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
     8941 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
     9001 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
     9061 ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg tcaggttttg
     9121 cctgcgaatg cagtaacaat ccagtcattt attaatggtt tttataacga acatccaggt
     9181 tcggaaatta atttaattgc gtgcagagaa aatagcaatg cgctattgat aaaaatatga
     9241 ccatgctcgc agttattaac tttgtgtaat tttaggaatt tataaagtta tatataacaa
     9301 atcccaataa ttaagttatt gggatttgtc tggtgaatta tttgtcgcta tctttccccg
     9361 ccagcagttt atccagctca tcgccaccga cgtgacggaa atcctgcccc ttcacgtagt
     9421 agaagataaa ctcacaaata ttctggcagc ggtcgccaat acgttcgata gaacgcgcgc
     9481 agaacagcgc agtaagtacg ctcggaatgg tacgcgaatc ttccatcatg taggtcatca
     9541 gttgacgaac aataccttcg tattcctgat cgactttttt atcttcacga taaatacgta
     9601 ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg
     9661 tatgacggcc cagcgactcc agacttacca gcaacggctg atgctgctgg gagaatttct
     9721 ccagcgcagt acggcagatt ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg
     9781 ttttactgat caccataacc agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa
     9841 tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt gacgttcttg tcgccttcga
     9901 tcacgcgctt cgccagatcg ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct
     9961 gctgctccac catgccgccc atggtcatca cctgcgtgcg gatactttcc agttcggcgt
    10021 tgaactggcc ggaaatatgt ttattaagat tgagactgtc cataacgcac tcctgaatca
    10081 accgtaacga ccggtgatgt agtcttctgt ttgtttcttc gctggcttgg tgaacagatc
    10141 gtccgtgttg ctgaactcaa tcaattcgcc caggtacata aacgccgtgt ggtcggaaca
    10201 acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc
    10261 ggtgatcagc tcttcaatac gcccggtaga gatagggtcg agcgccgaac acggttcgtc
    10321 gagcagcagc acttccgggc gaatggcgat accacgcgca atacacagac gctgttgctg
    10381 accaccagag agagagtaac cgctctggtg caatttatct ttggtttcgt tccacaatgc
    10441 ggctttggtc aatgcccact gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa
    10501 cagacgaacg ccaaaagcga tgttgtcgta gatggacatc ggaaacggcg tcggtttctg
    10561 gaacaccatg cccactttcg cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt
    10621 gtcgccatca agcagaattt caccttccgc acgctgctcc gggtacagtt caaacatttt
    10681 gttgaaggta cgcagcagcg tcgatttacc gcagccggac ggcccgataa acgccgtaac
    10741 ctggttttta gcgatatcca ggttgatgtt tttcagggca tggaatttgc cgtagtagaa
    10801 gttcaaatta cgaacctgaa ttttactcgg ggcagtttca accatactca ttgcaatctt
    10861 ttcctcatcg tgcctgatgc acttcgctaa tcaggcttac cgtaggccgg ataaggcgtt
    10921 cacgccgcat ccggcaaagt gttaaccgtg tttattcttc gcaaaaacaa cgcgcgccag
    10981 aatgttcagc agcagtacgc acagggtaat aatcaatacc ccggcccagg ccagttgttg
    11041 ccattccgca aacgggctca tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg
    11101 ctgcatcatg tccgtgctcc agaactggtt ggagagcgcg gtaaacagca gcggcgcggt
    11161 ttcaccagca atacgggcaa tcgccagcag gataccggtc atgatcccgg acaccgacgc
    11221 tttcagcgta atcgcggaga tcatcttcca cttcggtgta cccagcgcat aagccgcttc
    11281 acgcaggctg tccggcacca gtttcagcat gttctcggtg gtgcggataa caatcggcac
    11341 ctgcaacagc gccagggcaa tcacgcccgc ccagccggag aagtgctcca tctgcgccac
    11401 cacgatggtg taaacaaaca gaccaaccac aatcgacggc gcggagagca gaatgtcgtt
    11461 aatgaagcga atcacttctg ccagccagga tttacgacca tattccgcca gataaatccc
    11521 cgccataatg cccagcggcg taccgaatac cgtggcccac aaaattaaca gcccgctacc
    11581 cgccagggcg ttcgccagac caccaccttc cgtattgggc ggcggcgtca tttcagtgaa
    11641 cagcgccagc gacataccgt cgataccgcg agtgatggtg gacattaaaa tccagatcag
    11701 ccagaacagg ccgaaggcca tcgtcgccat cgagagcgtc agcgcaatac ggtttttgag
    11761 gcggcgacgc gcctgcattt tgcggcgaga ttcagccagc gccgcagtgg tttgcatttc
    11821 aaccatagcc attagcgtgc cccctcattc ttagccaggc gcataatcat aaacttcgat
    11881 gcggcgagga cgatgaaggt aatcacaaac aggatcaggc ccagttccat cagtgcggca
    11941 acgtgcagac cggattccgc ttccgcaaat tcgttcgcca gcgcagaggt gatactgttg
    12001 cccggcatat acagcgaggc gctgtcgagc tggtaggtgt taccgatgat aaaggtcacc
    12061 gccatggttt caccgagcgc gcggcccagc cccagcatga tgccgccgat aacaccattt
    12121 ttggtgaacg gaagaacgat acgccagata acttcccagg tggtgcagcc aataccgtag
    12181 gccgactctt tcatcatcac cggggtttgt tcgaacacat cacgcattac cgccgcaatg
    12241 tacggaataa tcatgatggc gaggatcacg cctgccgcga ggataccgat accaaatgcg
    12301 gggccagaga acagcgcgcc aacaatcggg atattcgaca tgatattgcc gaccggctcc
    12361 tggaagtaaa cggcgaatag cggcgcaaag ataaacaggc cccacatgcc gtaaacgata
    12421 cttggaatgg ctgccagcag ctcaatggcg atacccagcg ggcgtttcag ccagccaggc
    12481 gcaagctcag tcaggaacag ggcgataccg aaactcaccg ggacggcgat cagcagcgcg
    12541 ataaacgaag tcaccagcgt accgtagatc ggcaccagcg ccccgtagat atcgttcggt
    12601 gcatcccact ctttggtcca gaggaaagcc agaccaaatt tctgaatgct cggccaggag
    12661 gagatgatca gagagacaat aatgccaccc aacatcaata gcacaatcag cgccgccagt
    12721 tttaccagca cgctgaaaat tatgtcgccc tttttacccg gtgggttaaa agcaggcttg
    12781 gttgcagcca taagttactc ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg
    12841 atgcggcgta aaacaccgta cccggcctgg agttttatta gtacagcggc ttaccgctac
    12901 tgtctttaat attggtcttc cacgcagcgc gaacctgttc aactacacta tccggcaggc
    12961 tggcgtaatc caggtcgttc gcctgtttag ccccggtttt gtacgcccag tcgaagaatt
    13021 tcagcacttc tgtgccttgt tctggtttct tctgatcttt gtggatcaga atgaacgtgg
    13081 tagaggtaat aggccatgca tcttcgcctt tctggttggt cagatcctga gcgaaggttt
    13141 tgctccagtc tgcacctttt gctgcattag cgaagttttc ttcggtcgga ctaaccggtt
    13201 taccatcagc ggagatcagt ttggtgtacg ccaggttgtt ctgcttcgcg taagcatatt
    13261 caacataacc aattgcaccc ggcagacgct gaacgaacgc ggcgataccg tcgttacctt
    13321 taccgcccag accgatcggc cattttacgg tagagccagt accaacgttg tttttccact
    13381 cttcgttcac tttcgccagg tagctggtga agacgaagga agtcccggag ccatctgcgc
    13441 ggcgtactac agcaatgttt tgtgaaggca gtttcagacc cggattcagt ttggcgatgg
    13501 cttcatcatc ccacttcttg attttgccca ggtagatgtc gccgagggtt ttaccatcca
    13561 gcaccagttc gccagacttc agccctggaa tgttaaccgc cagcaccacg ccgccaatca
    13621 cggtcgggaa ctggaacaga ccttcctgag ccagtttttc gtcagacagc ggcgcgtcag
    13681 aggcaccaaa atcaacggta ttagcgataa tctgttttac gccaccggaa gaaccgatac
    13741 cctggtagtt aactttatta ccggtttctt tctggtaagt gtcagcccat ttggcataca
    13801 ccggcgcagg gaaggttgca cctgcacctg tcaggcttgc ttctgcaaac acagagaaag
    13861 cgctcatcga taaggtcgcg gcgacaacag ttgcgacggt ggtacgcata actttcataa
    13921 tgtctcctgg gaggattcat aaagcattgt ttgttggcta cgagaagcaa aataggacaa
    13981 acaggtgaca gttatatgta aggaatatga catttttatg acagaaagga attaccaaat
    14041 aacttactga ttttaaatgt gaagatgaaa atcccacatg tcatcttttt gtcatgatgc
    14101 atttaaaagt gataaagaaa tgggtgagat gacaacgatg tgatgaacaa tcgttgtcag
    14161 acctattagc gcacaatctc aacagttatc gtcgctgttg cagtaaattg ccccggggct
    14221 gggcgagctc cagtaaggct cgcaggagca gctgaaaaag ttacactacc tgatttttca
    14281 ccatacactg ttgatttacc catgtctgtt ggcaactcac caccgttcac tttgatttcc
    14341 ttattatttt tgtcgtaaat ttttatcccc acgttagagt ttgaagttac gaccattgaa
    14401 ttgtccgtat tgggttcggt agcataaaaa gacgtactga tatcatcggt attcagtatc
    14461 cccaggtttt cacactcaat agtgaccgtt ttagtaattt tatgtgttgt taccgcactt
    14521 ccaacaacag atgaaaaatc tgcagctggt atttctttaa aatcaaaata aactggctgt
    14581 aaattattta tttcgcaact ttgcggagct gtaatatcgc cccgaatata aacatcagcc
    14641 atcggctgtg agtaatccac cggggtgtca cggctaattg ttccatacaa tgtcactatc
    14701 tttgtttcag gaataatcaa ttttcctgca aatgtcttct tgatataaaa atatactatc
    14761 gctttagccc cactagatat acttaaaggt tcaatgcggt tgcaattgta ctttgtatta
    14821 ccagacgggt tgttagcatg atattcaaaa ggtgcattca catacccaac cccaagaata
    14881 tctatagcaa tcccaacatc aaggttcgga ttaagatcat aataattaat attagagtga
    14941 accccagcat aagttaatgc ctggtttatt tttgacgtat aatagatata ggggaattct
    15001 cctacgttac cagtatcaca atgacagcgt ccaggatatg aggatccatt agttacagtg
    15061 gtgttaaatg tcgttccaac cttattttcg ttcgcggtaa taagatatcc cgagaaatca
    15121 agcgtactgt gaaatacgcc attatcaggg gtacagatac ctttaggagc tgcaagtgct
    15181 acactactta acgagagcat aaacacagca aagcaccact gtttgatata cttgttcata
    15241 aaaatccttt taatgacatg tcacaccctg ttcaattaat ccgctctcac tttctggtgt
    15301 cgaaagtgaa taattgaaga tgcattttgt tgaggcgttt tctccccagg ataacgtaac
    15361 ctgtcctttt tctggtagac cagaaagata aagttcgcca cggtcaccga caattcctga
    15421 atctgccaaa cttgcatctt gtatcgcagc aatggctcca aaaggtaccg gtttaccatt
    15481 tgcatttaac actctgaaga gaacgcgata acccacatga gtcacaaact ccgctttaac
    15541 cactgcacca cgcgtgggaa ccatcgtaac gacggtatta tcaagttcta tattcgactt
    15601 cgagaaatag ttatcgctga ttgctacccg attttcatca taagggttta gctgtgtctt
    15661 gaccgtatag ccgcgccagt ccgtcgcagc accgcgcata ttatctattt ctaacccggc
    15721 ggccccaggt gctttaacaa gagcaatagt ttcacctaac tcctgactta acgttacccc
    15781 ttcgctgtgt gccagaattc cccctctgat accataattc agcctctggg aattttggct
    15841 gtagttatag ctgccgttca ccgtgccata agagccatca taggtcaggg acatattacc
    15901 gccaacctca tcatcatgac tgttatagga ctgcattaaa ttccatgaaa ggttatttct
    15961 ttccagcgcc gtaccgctga gtccgagttg atgtgagcta ccggaattat cgttttgcga
    16021 aacagaatag gtggcgtaac tattacccca ccagcccgat aaaggaacag ataaagagag
    16081 catgtagatc tgctcactgg tgttatcact ggcatggttg ttccccgtcc cctgtctggc
    16141 agtattggaa taactccaag agacgttcca cgagagacgc ttccagctac tgctgtagcc
    16201 aaattgcatt aatcgttcga catcatcggt atgccagtat tgttgccggc ttaatgtcag
    16261 gtataaattg ccataatctc tccccaacga ctgcgttagc gttccctcca cgcgactacg
    16321 tcggttacct gtttcccaaa agtcttcagg gctgttgcgc cgcgatgccc actcattgag
    16381 ggtatagtag ccctcggtgg agtaacgata gccaaccagg cgcaagctag tgtctgtgtc
    16441 gtcaaatcgt tttgaataga gaaagcgata tgactgaccc gtttctgtat catcatggct
    16501 aaaattcgca cgagcatgtg tcacatcgaa agaaattgcg cccaaagcgc caagatcttt
    16561 accgacaccc agagctgcag aaccataatt ttcagcagcc tgcatacctc catataaagt
    16621 gatcccgtgg gaaaaaccat gaagtatttg cgcctgaagg acgtccggtg taaatccgtc
    16681 ttcatcgcgc aattcaccca cgctgacatc aacatctgtc tgaccttcac gtttaagaat
    16741 cgccaatgaa gcataaggtt gggtgaatcg ttgttcgcta ccgtcttctt ctttgatggt
    16801 tacgtccaaa tcgccacctg tactggcggt gttgagatcg gtgatctcaa aagcaccttg
    16861 tggaacggca gactgataaa tcacgtagcc attctgccga atgatgacgg tggcgttgga
    16921 ctttgcaatc ccacgaacca caggagcaaa gccattctgg ctggcgggta gcatatcgtt
    16981 atcagtatac aaacgcgcgc cgcgaatttg cgtactgtcg aaaatatcgc tcgccgtcca
    17041 cgtatcacca atcataatct ggcttcttaa cgcggcaata tcgcgctgta tccaggtact
    17101 aatggagttg tattccgcac ggccatcgct ggtgtttaaa gtactgtaat tacgtagccg
    17161 ccacggtcca atattcagtc cattgcgcag attgagataa ctggtatctg tctcttgtga
    17221 accataatct gaacgtagtg tatgtgaacc agaaaaatcg tagttgatgg tgagcgcgtt
    17281 aatcccctca tcccaacgcg tggggctgat gtaatcataa gccatcgcgt ccatgtagat
    17341 ttggggaatg gatatattca gctgtaattt atcactgtca aactgccagg atgagccttc
    17401 aatacgtgga gcaggatcga ggcaaaccgt ctcatccaca tcattgaacg atttgagcgc
    17461 atcaactttt atgccaaagg aggacagtgt ttttttaatg gcaataaagc aagcgtgcag
    17521 atcagcatct ggattatcct gaacaaatgt cacatcctga cgttcaaaca ttttaccgtt
    17581 gataaaaata tcaacgctat aaacacctgg gggcatcagg tttgcccgat taaactggcg
    17641 aatatcgact ccggattgat ggtcgatttc cagaagcgcc ggattgaata cactgacctc
    17701 ggccatcagg ttttgcgaga aaatcagaca cgtccctgcc gtgaggccaa ccactattct
    17761 ggtcgtcatc ataatacgcc tatccttatc ccttgcttac agcgtcattt ttatcgggtc
    17821 actgactgcg ccgtaatcat taataaatcg ccaggtcatt tggcctcccg cagaattagc
    17881 aggtaaaggt aacgtgagcg tttcaaaagg acggatcata ttgaggggca catcatggcc
    17941 atcaacgatg acatccatca cactgatatg aattgggttg ggattctctg ctatcagatt
    18001 tcgcccttcc cggcgccatt tcactttcgg cgcagcatct tcgggaataa ggcggatatt
    18061 tgctggtcgc cagaacagct tgatacgcgt ttttactgct atttccagcg agttcgtcgc
    18121 tttgttgctc gctgatggcg gaatattttt aatattcagc cagaagactg actcgcgatc
    18181 atcaggcaat gacacacctt tggtgtgaat aatgcgtaat attttttctt gcgcaggttc
    18241 taatcgtgat actggcggaa taacggtaaa cggcgctttg cttttatcct ctttattaaa
    18301 tggatctacc caagcctgga tgagataagg tattttttct tctttgctaa atacagaaat
    18361 agatttatca gcattattac ttaagtaaac cacccgcgta ccaccaatga caacgcctgc
    18421 ataagttaat gctggttggc ctaaaaaaac gctgagtata atcgcggtaa ataacttaca
    18481 tgaaactgtt gttttaaaga gcctgattaa acaacttttt ctggacataa cgactccctg
    18541 gccaaagcca actgaataaa aaggcccttg ataaattatt tcttatattc gacagtaaat
    18601 tgtgaatcag cgttcgctgt accagccgtt acggtcgcat tggttgccac atagcgtgca
    18661 ataaacttca tttcagcctt gccttcagca tcgatagcgg tataagcaga gtcgttataa
    18721 agtttaacct gcgaattatc ggactggtta aaaatacgaa taccaacgcc ggttgccgca
    18781 atcgcttgtc ctgtaccggt aaaatcacca gggttgcctg ctgtaagcgg cgtacccact
    18841 ttcaggtcgc cgtcgccgcc aggcgcttct gtaccatcaa aacgaacggc tgcttgagtg
    18901 tacgttgccg ggcaattttc gagcttaata ctgaacttct gtggcgacgc cagactacct
    18961 acacctgaaa atgcattttt actgacttta cccagtggga caatttgatc ggctgtatcg
    19021 gaggtaactt cacaagtagt gtcgacaatt tcaccgacga agtgaacaac accatcttct
    19081 gccagtgcat gcccggccag cattagagat gccagagtga atgcaccgcc tataatatta
    19141 cgcttcataa aaactccatt gatataatat atcctttacc atgaatcatc acttatatta
    19201 ataagtgtat ttttgattca aaggaatagt acaccaaaga gacagcttta tctaaaaata
    19261 atattaatta cgcaaaaata aaatataaat ttattgttta aatattattt aaacagtaaa
    19321 aaatcaagtt tgttatcaga cattaatgat aattcagaat atatttatat agaaagatag
    19381 atgaatttat tgatgtcagt tataaaaaag ggccaccggt cggcagccct tttttatcta
    19441 tgatagtgat tactcaaccg taaccgattt tgccaggtta cgcggctggt caacgtcggt
    19501 gcctttgatc agcgcgacat ggtaagccag cagctgcagc ggaacggtgt agaagatcgg
    19561 tgcaatcacc tcttccacat gcggcatctc gatgatgtgc atgttatcgc tacttacaaa
    19621 acccgcatcc tgatcggcga agacatacaa ctgaccgcca cgcgcgcgaa cttcttcaat
    19681 gttggatttc agtttttcca acagttcgtt gttcggtgca acgacgataa ccggcatatc
    19741 cgcgtcaatc agcgccagcg ggccgtgttt cagctcgcct gcagcgtagg cttcagcgtg
    19801 aatgtaagag atctctttca gcttcaatgc gccttccagc gcgattgggt actgatcgcc
    19861 acggcccagg aacagcgcgt gatgtttgtc agagaaatct tctgccagag cttcaatgcg
    19921 tttgtcctga gacagcatct gctcaatacg gctcggcagc gcctgcagac catgcacaat
    19981 gtcatgctca atggaggcat ccagaccttt cagtcgagac agttttgcca ccagcattag
    20041 cagcacggtg agctgggtgg taaatgcttt ggtggaggcc acaccaattt ccgtaccggc
    20101 gttggtcatc agcgccagat cggattcgcg caccagagaa gaacccggaa cgttacagat
    20161 tgccagcgaa ccaaggtaac ccagctcttt cgacagacgc aggcccgcca gggtatccgc
    20221 ggtttcgcca gactgtgaca aggtgatcat caggctgtta cgacgcacgg cagatttgcg
    20281 atagcggaat tcagaggcga tttcgacgtc gcacggaata cctgccagcg attcaaacca
    20341 gtagcgggaa accataccgg agttataaga agtaccacag gcgaggatct gaatatgctc
    20401 aaccttcgac agcagttcgt cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg
    20461 gctgatgcgc ccggtaaggg tgtttttgat cgcgtttggc tgttcgtaga tctctttctg
    20521 catgtagtga cggtaaatac ctttatcgcc cgcgtcatat tgcagattgg attcaatatc
    20581 ctgacgtttt acttccgcgc cagttttatc gaagatgttt accgaacggc gagtaatttc
    20641 cgcaatatcg ccctcttcaa ggaagataaa gcgacgggtc accggcaaca gcgccagctg
    20701 gtcagaagcg ataaagtttt cgcccatacc caggccaatc accagcggac taccagaacg
    20761 tgccgccagc agggtatccg ggtgacggga gtccataatc actgtaccgt acgcaccacg
    20821 cagctgcggg atagcacgca gaacagcctc acgcagagtc ccgccttgtt tcagctccca
    20881 gttcaccaga tgggcaatca cttcggtgtc agtttcagaa acgaaggtat agccacgcgc
    20941 ttttaactct tcacgcagcg gttcatggtt ttcgatgatg ccgttatgca ccaccacaat
    21001 gtgttcagaa acatgcggat gcgcattcac ttctgaaggt tcaccgtggg tcgcccagcg
    21061 agtatgagca ataccggtgc cgccatgcag aggatgttct tccgctgcct gagccagcat
    21121 ctggacttta ccgaggcgac gcaggcgggt catatgacct tctgcatcaa caacggccag
    21181 accggcagag tcatatccgc ggtattccag acgacgtaaa ccttcaagaa ggatttctgc
    21241 tacatcacgt tgcgcgatcg cgccaacaat tccacacata gtttttgatt ccgatttata
    21301 tcgttgtcgg tcaacctgta tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg
    21361 ggttattttt atagttactg cttgtgggag ggagattatg ttatctcctc atcccagatg
    21421 accggattac ccggcctgaa tcactttttc tttaccggac gacgccagcc ttctttctga
    21481 gtctgcggca cacggctgat agctaatgca ttttcgccga cattacgcgt cacagttgta
    21541 cccgcagcaa tggtcgcgcc tttgcctact gttaccgggg ccaccagctg agtgtcggaa
    21601 ccgacaaaca catcgtcgcc gataatggtc ttaaatttat tcgcaccatc gtagttgcag
    21661 gtaatggttc ccgcgccgat gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc
    21721 agatgcccgg ctttcgagcc tttacccaga cgcgcttttt tcatctcaac gaagttaccg
    21781 acgtgtgcac cttccagcaa ctcagcaccg ggacgcagac gggcaaacgg gccaatagta
    21841 caggccgctg ccagattcgc gtcttccaca acggtatacg gactgatttc gcaatcatcg
    21901 ccaatcacgc tgtttttaat cacgcaaccg gtgccgattt tcacgcgatg accgagagtc
    21961 acgttgccct cgatgataac gttagtatca atttcaacat cgcgcccgtg agtaagcgta
    22021 ccgcgcagat caaaacgcgc cggatcgcgc agcataacgc ctgctaacag cagtttttca
    22081 gcctgttcgg actggtaaac gcgctccaga cgagagagtt gcaggcggtt attcacgcct
    22141 tcaacttcgc ttaaacgttg cggatgaacg gcgacgattt cacgtccttc ctgatacgcc
    22201 agcgcaataa tgtcggtgat gtagtattca ccctgagcgt tattgttggt cagcttcgcc
    22261 agccagcgtt tcatatctgc gccgttagcg atcagaatgc cggtgttgat ctcctgaatc
    22321 tgacgctgct cgtcggtggc atctttgtgc tcaacaatgc cggtaacttt gccgttttca
    22381 cgggtgatac gtccataacc ggtcggatca tccagtttca ccgtcagcag accaatgcca
    22441 ccctgcggtt tagcatcacg cagacgctgg agtgtttcca cagagatcag cgggacgtcg
    22501 ccgtagagca ttaaaatgtc ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca
    22561 tgacccgtac ccagctgctc tgcctgaagc acccagttca ggttgtcgtc tttcagcgcc
    22621 tgttttagca gatcgccgcc gtgaccgtac accaggtgaa cgtgcgctgc gcctaattca
    22681 ttcgcagcat caatgacatg ctgaaccatc gctttcccgg caagggtatg cagcactttc
    22741 ggaagatcgg aatacatgcg cgtgcctttg cctgcggcaa ggatcactac gctcatagca
    22801 ttattcaaca tacgcgtcct gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga
    22861 aaaaactaca tttttttcat cgtgaaatgg acagaggata aattgttcaa taacggatta
    22921 tccccgctga cgaaagtgcc attttcgacc atcgtcacgc cttttgtctg cagaaaatta
    22981 aggcagtacc ttcatttaag cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat
    23041 aaacgggaaa acaggggtgt tttatatcag caggatctat gtgaacgctc ttcaggacgg
    23101 atcacgcgta aaaaaaagcc agcctgtttc cagactggct tttgtgcttt tcaagccggt
    23161 gttacatcgc ttttttggtc aactcgataa cgcgcagctg cgcgatcgct ttggccagtt
    23221 ccgcagacgc ctgagcgtaa tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct
    23281 tacgtttcgc ttccatggct cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg
    23341 ccagaacggt cacgttgcca ggctgcactt caagaatgcc gccagacaga tagataaact
    23401 cttcgtgacc gtgctgtttc acgatgcgaa tcataccagg cttaatggcg gtgagcagcg
    23461 gtgcgtggcc agggtagatc cccagttcac cttcgctacc cgttacctgg attttctcga
    23521 ccagaccaga gaacatttgt tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca
    23581 tatcaccctc cgattaaggc gttaaagttt tttggctttt tccacagctt cttcgatgga
    23641 accgaccatg tagaacgcct gctccggcag gtgatcgtat tcgccttcca tgatgccttt
    23701 aaagccacgg atggtgtctt tcagggagac gtatttaccc ggagaaccgg tgaatacttc
    23761 tgccacgaag aacggctggg acaggaagcg ctggatctta cgagcacgcg ctaccaccag
    23821 tttgtcttct tcagacagtt catccatacc caggatggcg atgatgtctt tcagttcctg
    23881 ataacgttgc aggatggact gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac
    23941 cagcgggtcc agctgacggc tggtggagtc cagcgggtca acggccgggt agatacccag
    24001 agacgcgatc tgacggctca gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg
    24061 agacgggtca gtcaagtcat ccgcaggtac gtatactgcc tgtacggagg tgatagaacc
    24121 agttttggtg gaggtgatac gttcctgcag aacgcccatc tcttccgcca gggtcggctg
    24181 ataacctacc gctgaaggca tacggcccag cagtgcggat acttccgtac ctgccagggt
    24241 gtaacgatag atgttgtcaa cgaacagcag aacgtcacga ccttcgtcac ggaatttctc
    24301 agccatggtc agaccggtca gagcaacgcg cagacggttt cccggcggct cgttcatctg
    24361 gccatacacc agggatactt tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc
    24421 gttaccctca cgagtacgtt cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat
    24481 cgcgatgtta cgaatgagct ccatcatgtt tacggtttta cctacacccg caccaccgaa
    24541 cagaccaact ttaccgccct tagcgaacgg acacatcagg tcgataactt tgataccggt
    24601 ttccagcagt tcctgagagt ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc
    24661 ccaacgctct tcttcaccga tctcgccttt catgtcgacc ggttcaccca gtacgttcat
    24721 gatacggccc agagtcgctt tacctaccgg gacttcgatc gggtgttcga ggtcttttac
    24781 atccagaccg cgacgcagac cgtcggagga acccattgcg atggtacgca cgataccgcc
    24841 gccgagctgc tgctgaactt ccagcaccag acgctcatta ccattttgca cctcaagagc
    24901 atcgtacacg cgcggtacgg catcctgagg gaattcgacg tcaactacgg cgccgattac
    24961 ctggacaatc tttccagtag ccatcttaaa tcctctacga aataacctgt ttaaaccgcg
    25021 gcggcccccg agacgatctc ggtgagttcc tgagtaatgc tggcctgacg agctttgttg
    25081 tataccaact gcagctcttt aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc
    25141 accatacggg cggcctgctc gctggccagg ttttcaacca cgccctgata aacctgagat
    25201 tcgacataac gacgcagcag ggtatccagc aacgccttcg gatcgggttc gtacaggtaa
    25261 tcccaggatt tatgtttcag atcatcatca tctgatgccg gtaacggcag cagctggctg
    25321 atggtcggaa cctgagacat ggtgttaata aatttgttgc tgacaatgta aagcttgtcc
    25381 agacggcctt cgtcgtaggc ctgcaacatc acttttaccg gaccgatcag ttcggacagg
    25441 gaagggttat cccccatgcc ggtgacctgg gcaacaacat tgccgcccac ggagttgaag
    25501 aacgacacgc ctttcgagcc gatcattgcg aggtcgcatt gaacgccttt gtcggtccag
    25561 gtcttcattt ccgccagcag ttttttgaac aggttaatgt tcaaaccacc gcacaaacca
    25621 cggtcggtcg acaccaccag gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg
    25681 tgcttatatt ccagattacc gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca
    25741 taaggacggc tggccgccat gcgatcctgc gatttacgca ttttggaagc ggcgaccatc
    25801 tccatcgctt tagtgatctt ttgcgtgttc tggacgcttg cgatcttact acgtatctct
    25861 tttgcgccgg ccatgagctt ctcctcaatg ccttgcggcc tgccctaagg caagccgcca
    25921 gacgttacca ggattgggtt gctttgaagg aatcgaggat gcctttcagc ttgccttcga
    25981 tttcgtcgtt gtagccaccg gtctggttga tctcttgcat caacggagcg tgatcacggt
    26041 cgacgtaagc cagcagagcg gcttcgaagc tgccaatttt cgacagttca acatccgcca
    26101 ggtaaccacg ttctgctgcg aacagaacca gagactgctg cgcaacggac atcggcgcat
    26161 actgtttctg tttcagcagt tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg
    26221 catcgtcaag gtcggatgca aactgagaga acgctgccag ttcacgatac tgtgccagag
    26281 cggtacggat accaccggac agttttttca tgatcttggt ctgtgctgca ccaccaacac
    26341 gggatacgga aatacccggg ttaaccgcag gacgaatacc ggcgttgaac aggttggttt
    26401 ccaggaagat ctgaccatcg gtaatggaga ttacgttggt cggaacgaac gcagaaacgt
    26461 cacccgcctg agtttcgata ataggcagtg cggtcagaga accggttttc cctttcactt
    26521 cacctttggt gaaggcttca acgtattcgg cgttaacacg tgcagcacgc tccagcagac
    26581 gagagtggag gtagaaaacg tcgcccggga atgcttcacg tcctggcgga cgacggagca
    26641 gcagggagat ctgacggtaa gcaacagcct gtttagacag gtcatcgtaa atgatcagcg
    26701 catcttcacc gcggtcacgg aagtattcgc ccattgcgca accggcatac ggtgccaggt
    26761 attgcagtgc agcggattca gacgcggttg ctaccacaac gatggtgtta gccagtgcgc
    26821 cgtgctcttc cagtttacgt accacgttag aaatggtgga cgctttctgg ccgatagcga
    26881 catagataca tttgataccg gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg
    26941 ttttacctgt ctgacggtca ccgatgatca attcacgctg accacgaccg attgggatca
    27001 tggagtcaac ggctttataa ccggtctgta ccggctgatc tacggactga cgttcgataa
    27061 cgcccggagc gattgcttct acagcagaga agccgtcgtg atccagcgga cctttaccgt
    27121 cgattggtgc acccagagtg ttaaccacac ggcccagcag gccacggcca accggaactt
    27181 ccaggatacg gccagtacac ttaactttca tgccttcggc aaggtcagcg tacggaccca
    27241 taacaaccgc acctacagag tcgcgctcga ggttcagtgc gatagcgtaa cggttacccg
    27301 gcagggagat catttcaccc tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt
    27361 cacttacaga aacaatagta ccttcgttgt gagcttcact cacaacattg aactgagcaa
    27421 tgcgctgctt gatcagttcg ctgatttcgg tggaattcag ttgcatgctc cagtcccctt
    27481 aagactgcaa gacgtctgca aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca
    27541 tatcacccgc tcggatgata acgcctgcca ttacagactt atcgattttg caattcagct
    27601 taactttgcg tgacagacgt ttttccatcg cagcagaaat tttcgcgagc tgttgttcac
    27661 tcagtgcggc agcggaaatg acgtctacct cagcggtagc ctcactcacg gcacgaaggt
    27721 gaataaactg ctccagaaca tccgggagcg cgttaagacg accattttca gccattaccc
    27781 gaatcaggtt ctgaccgttt tcgtccagtt gctcaccaca aactgcgata aacgactcgg
    27841 cgagcgtttc tggcgcaagc gcgccagaga gaagctctgc catttgttcg tttttggtta
    27901 cctcggcggc aaacgccagc atgtcctgcc agcgttctac actttggtgt tcgacggcaa
    27961 agtcaaaagc tgctttggcg taggggcgag ctaccgtaat aaattcagac atcagcccct
    28021 ccctccttac agttcagcga caagtttatc cacgatgtcg ctgttagcag cttcatccac
    28081 ggaacgttcg atgatcttct cggcgccagc aacagccagg atagcaactt gcttacgcag
    28141 ctcttcacgg gcacgtttac gctcggcttc aatttctgcc tgcgcctggg ccacgatttt
    28201 agtacgttcc tgttctgcct cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt
    28261 cgcctgctcg atgattacct gggcttccgc tttcgctttt ttcagctggt cggtcgcgct
    28321 ggcctttgca aggtcaaggt ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat
    28381 ttctttttga cgtttttcga tggctgccat taatggcggc catacgtact tcatgcagaa
    28441 cagaacgaac aggacaaacg cgatggcctg gccgaggatt gttgcgttaa gattcacagc
    28501 acaatgcctc tatttagtta acgttctgat attgctcttt aaataaaagc aacgcttact
    28561 acgcgacagc gaacatcacg tacagaccca gacctacagc gatcatcggg atagcatcca
    28621 ccagacccat aacgataaag aactgagtac gcagcagagg aatcagatca ggttgacgcg
    28681 ctgcgccttc caggaattta cccccgagga tgccgatacc gatcgcagca ccgattgccg
    28741 ccagacccat catcacagcg gcagccatgt acagcagatc catattcagg ttttccatga
    28801 cagtctccag tttgtttcag ttaaaacgta gtagtgttgg taaattaatg ttcttcagac
    28861 gccatcgaca gatagacgat cgtcagaacc atgaagatga aggcttgcag cgtaatgatc
    28921 aggatgtgga aaatggccca cggcacattc aggatccact gtgaccacca cggcaacaga
    28981 ccagcaatca gaatgaaaat cagctcaccg gcatacatgt taccgaacag tcgcaaacca
    29041 agtgaaactg gtttggacag caggcttacc ccttcaagga ttaagttgac aggaatgaac
    29101 gcccagtgat tgaacggctg cagcgtcaac tctttcgtga agccgccgat gcctttcatt
    29161 ttgatgctgt agaacagaat caggataaat acgcccaatg ccatagacag cgttacgttc
    29221 acgtccgcag acggaaccac acgcagtgca ggcagaccca gtacatgttc agcaatgtac
    29281 ggcagcaggt cgataggcag taaatccatc aggttcatca ggaataccca gacgaagatc
    29341 gtcagggcca gcggagcaat cagcttgctt ttgccatggt acatgtcttt cacgctacca
    29401 ttaacaaagc cgatcaccag ctcaatcgcg gtctgaaact tacctggcac accgctggtc
    29461 gcctttttgg ctacgctacg gaataaaacc aggaacaaca gacccagcac caccgagaag
    29521 aacatggagt caatattgat tgtccagaag gtggctgggg ggttttgtgg atccaccagc
    29581 gagaatgtac gcaggtccag ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc
    29641 gtcatatttt ctgaagccat gatgcctttt accctttgtt gttaattaca gccggtgcca
    29701 gtatctgaac caccagcacc aaaacccacg taacgatcag cggcaagaat accgccttta
    29761 aaaccgccaa cgccaccacc agtaacacca acatcgccag aactttgaaa gcttcgccaa
    29821 atgcgaatgt ccaggccacc cggcctttcg ctggtgtatg cgcctggtga cgccaggcaa
    29881 atatcataaa caaaacgtta ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc
    29941 cccagaaggg gtctttgagg ctgaacagca atccacttgc tatcaccacc agtaactgaa
    30001 cgagcagaag cttccgagca acgtttcgac tcacgagcga cacagacatc acgtttttca
    30061 ctcctgctcc cttcgaggta tgccgcgtgt cgtataaaac tttctttaag gcttagagtc
    30121 aagcatcaaa aagcggtcaa attatacggt gcgcccccgt gatttcaaac aataagtagc
    30181 caaaaggtga ataaatgttt aaatattttt ccagtgcata caattgcgac ttttctgcta
    30241 accctgttcg atcatgaaaa actgtaaata acgcgtaaac actggtgata aagcgtgctt
    30301 cagatcacat attgcgcatg ttcgcgcaca gcatatttat ttacttggca aatgatgcct
    30361 ttgcaagttt atgatatttc agtctaaaaa cagatactgt tttaataaat gacatttaca
    30421 caacaaaaac cacccattga catttttaat aatgttttaa cagccaatga tggttcttag
    30481 cgccgatttt tagcagactg atattttcac taatgactta ttttctgctt accaaaaaaa
    30541 gccacgttat cttgttgatg caaaagagtg aacgtggcgt taaatgtaac cagttatatc
    30601 agtagaaaac ctggttgttg ttaacagtct aaccggtcaa ttttttatga tttttttgat
    30661 aaaaattaaa ttttatttgc tttaatcacc accagatgac gttcgccatc cagggctgga
    30721 acctgaagtt taaccactga ttcgacctga tattcttcgg gcaacaaagc gatttcatct
    30781 tccggcattt gccctttcag cgcgtagaaa cggccttgct caccaggaag atggtggcac
    30841 cagctcacca tatcgttcag agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc
    30901 tctgaaggaa actcttctac cctgctctgt actggttcaa tattctccag tttaagctca
    30961 tgttgcacct gacgaaggaa acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg
    31021 gcttcaggac gcacgataga gagtggaatg cctggcagtc ctggtccggt gccgacatcg
    31081 ataaaccgtt caccttgcag atacggtgcc accacaatgc tatcgagaat atggcgtacc
    31141 agcatctcat taggatcgcg gaccgaagtc aggttgtacg ctttgttcca tttatgcagc
    31201 atattcacgt aggcaataag ctggtttttc tggtgatcgg taagcgaaat acctgcgtct
    31261 ttcagcagta aggagagttt gttgagcacg gtgattacct gttcttgatg cgttgcctgg
    31321 taagcgggtg cttaccaggc atttttaatg cgttatgcgc tacgacgcag cataccctgt
    31381 tttttcagcc acaccagcag aatggagatg gccgcaggcg tgacgccaga aatacgcgaa
    31441 gcctggccga tagaggctgg tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga
    31501 ccggatacct ggcggtaatc cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc
    31561 ttttcgatct catcttgctg gcgcgcgata taaccttcgt atttaacctg aatctcaacc
    31621 tgttccgccg cctgttcgtc tgtcaatgca ggggcaaacg gcgtcagcgt ggttaatttt
    31681 tcataagtca tttccggacg acgcagcaga tcttcaccac tggcttcacg ggaaagcggc
    31741 gcagtcaggt gagcattcac ttcggctgca gcttccgccg acggggttac ccaggtcgat
    31801 ttcagacgct gacgctcacg ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt
    31861 tcgtcatcca ctaggcccag ttcacgaccg atttcagtca aacgcagatc cgcattatct
    31921 tcgcgtagca tcagacgata ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt
    31981 cctaaagtgc acaggtcatc aactagtacg ccgagatacg cctgagaacg tgccggagcc
    32041 caaccttctt tgtcagcaga cagacgggca gcgttaagac cggccagcaa accttgcgca
    32101 gcggcttctt cgtaaccggt agtgccgtta atctgaccag caaagaacag cccctggata
    32161 aacttgctct ccagcgtcgg tttcaggtcg cgaggatcga agaagtcata ctcaatggca
    32221 taacccggac gcacgatctt cgcgttttcc atcccctgca tagagcggac gatttgcatc
    32281 tgcacatcga acggcaggct ggtggagata ccgttcggat aaatttcatt agaggtcagt
    32341 ccttccggtt caaggaagat ctgatgctga tttctgtcgg cgaagcgcat gactttgtct
    32401 tcgatcgacg ggcagtagcg tgggccgaca ccttcgatca cccctgcata cattgggcta
    32461 cgatcgaggt tactgcggat cacatcatgg gttttctcgt tggtatgagt gatataacac
    32521 ggcacctgct ggggatgctg ggacgcattg cccataaacg agaataccgg cattgggtta
    32581 tcgccatgct gttgcgccag cacactaaag tcgatagttc gagcatcaat acgcggtggt
    32641 gtcccggttt tcagacgacc aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga
    32701 atggacggcg gatcaccagc acggccaccg ctgtaattat ccagaccgat atgaatttta
    32761 ccgtcgagga acgtcccaac ggtgagcact acggctttgg cacggaactt cagtcccatt
    32821 tgggtaacag caccgaccac gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg
    32881 aagatcatca ggttcggttg gttctccagc gccgtacgta ccgcctgacg gtagagcaca
    32941 cgatccgcct gagctcgggt agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta
    33001 aactggatac ccgcttgatc gatcgctttc gccatcagac cgccgagtgc atccacttct
    33061 tttaccagat gtcccttccc aataccgccg atcgccgggt tgcagctcat ctgccccaga
    33121 gtgtcgatat tgtgtgtcaa aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg
    33181 gcctcggtgc ctgcatgacc cccgccaatg atgatgacgt caaaaggatc cggataaaac
    33241 atggtgattg cctcgcataa cgcggtatga aaatggattg aagcccgggc cgtggattct
    33301 actcaacttt gtcggcttga gaaagacctg ggatcctggg tattaaaaag aagatctatt
    33361 tatttagaga tctgttctat tgtgatctct tattaggatc gcactgccct gtggataaca
    33421 aggatccggc ttttaagatc aacaacctgg aaaggatcat taactgtgaa tgatcggtga
    33481 tcctggaccg tataagctgg gatcagaatg aggggttata cacaactcaa aaactgaaca
    33541 acggttgttc tttggataac taccggttga tccaagcttc ctgacagagt tatccacagt
    33601 agatcgcacg atctgtatac ttatttgagt aaattaatcc acgatcccag ccattcttct
    33661 gccggatctt ccggaatgtc gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt
    33721 tttgcaccgg aatttttgag ttctgcctcg agtttatcga tagccccaca aaaggtgtca
    33781 tattcacgac tgccaatacc gattgcgcca aagcggaccg cagaaagatc gggcttctgt
    33841 tcctgcaatg cttcatagaa aggagaaagg ttgtccggaa tatctccggc accgtgggtg
    33901 gagctgataa tcagccagat ccccgaggca gataaatctt ccaacagcgg accgtgcagc
    33961 gtttcggtgg taaaacccgc ctcttccagc ttttcagcca ggtgttctgc tacatattcg
    34021 gcaccgccga gggtgctgcc gctgataaga gtgatatctg ccataaaccg ccacctttat
    34081 taagagtggc gtattgtacg ctgtgaacgc gttgggatct acctgtggaa aagtatggga
    34141 ttaaaaaagc cgattatggc ttgatggtac gcatgatcgg gttttgcagg acgatcaatg
    34201 tctcggtgga ctgaatttca tcaattgttt ggatcttgtt gataagtaca tgctggagag
    34261 cgtcgatcga acggcacatc acttttataa agatgctgta gtggccggtt gtgtaatagg
    34321 cttcagtgac ttcatcaagg ctttccagct ttgccagcgc ggaagggtag tctttggcgc
    34381 tctttaatat aatgccgata aagcagccta cgtcataacc gagctgcttc gggctgacat
    34441 caatacgcgc cccggtaatg atccccgcct gcttcatttt ctctactcga acgtgaatcg
    34501 tccccggact gacgccaaat tgtttcgcca gttcggcgta agcggtgcgc gcattgccca
    34561 ttaatgcttc caggatgcca cggtccagat tgtcgatcag ataattttcc ataggatttt
    34621 cttatgcaga ttgatgattc attctatttt agccttcttt tttaatgaat caaaagtgtg
    34681 ttaggctttt tattgaatga ttattgcatg tgtgtcggtt tttgttgctt aatcataagc
    34741 aacaggacgc aggagtataa aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta
    34801 gcttcgtgaa atctcacttt tctcgccaac tggaagaacg tctggggctg atcgaagtcc
    34861 aggcgccgat tcttagccgt gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa
    34921 aagcggtgca ggtaaaagtg aaagctctgc ctgatgccca gttcgaagtg gttcattcac
    34981 tggcgaagtg gaaacgtcag accttagggc aacacgactt cagcgcgggc gaaggactgt
    35041 acacgcacat gaaagccctt cgccccgatg aagaccgtct ttctccgttg cactcggtct
    35101 atgttgacca gtgggactgg gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc
    35161 tgaaaagcac ggtagaggcg atctgggcgg gaattaaagc aaccgaagct gcggttagcg
    35221 aagagtttgg cctggcaccg ttcctgccgg atcagatcca cttcgtacac agccaggagt
    35281 tactgtctcg ttatccggat ctcgatgcca aagggcgtga gcgggcgata gcgaaagatc
    35341 ttggcgcggt attccttgtc gggattggcg gcaagctgag cgatggtcat cgccacgacg
    35401 tgcgcgcacc ggattatgat gactggagca ccccgtcaga gctgggtcat gcgggtctga
    35461 acggcgatat tctggtgtgg aaccctgtac tggaagatgc gtttgagctt tcttccatgg
    35521 ggatccgcgt ggatgccgac acgttgaagc atcagttggc gctgaccggt gatgaagatc
    35581 gtctggagct ggagtggcat caggcgctgc tgcgcggtga aatgccgcag accatcggcg
    35641 gcggtatcgg ccagtctcgt ttgaccatgc tgctgctgca actgccgcat atcggccagg
    35701 ttcagtgtgg tgtatggcca gctgctgttc gcgagaacgt cccttctctg ctgtaataat
    35761 ttatcgccgc cagcgtctga gcaggcggct tcgcatcccg gtatcaaagc gccagatatg
    35821 atcgaaaatg cgcatgatgc cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg
    35881 atgctgatgt acccgctgca gctctttcac tttactcgtc acgtcgtcag gcaaccgctg
    35941 agcgataaaa tcagaaatca ccaccgcgtc ggcatcaaac cattccctgc tttgcaagcg
    36001 ttccataatg gcgcgaaaac aactggcaag atcggtgccg ccacgaaact gctggcttaa
    36061 aaaacggatt gcttgttcga tgccttgtgg gcctgaaagc tcataacgga cgatctcggt
    36121 ggaaaatagc ataatatagc agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag
    36181 gcagaacgct ttcgcacact gttcattaaa gccgcccatt gagccggaag tatccacaca
    36241 gacaataaac ggcccgcgcg gctgttcatc gtaatcttta tgtaccaccg gacgttcgat
    36301 cactttttca cgccacgact caccgtgcag gcgataggtg agcaactgtt tttccaccag
    36361 ccgacggtaa aactcatact ccagttccgt tatccctagt gtcgccagtt ctggcggcag
    36421 gagacgtaaa atatcatcgc tttgttgcag accatcaacc tgctcaggaa cagttgccgg
    36481 ttcgcgcacc atggtgcgga aggcttccat ctgcgcatcg ttacgcggta ttgatttggc
    36541 ttcccgggaa cgccccagct gttctgccag gcgtttcagt tccggctgtt cgttaagaaa
    36601 ttcaccgtat ttcacaatca actgatagtc gccacgttta agctgaccgg cgctcatatc
    36661 ccacagacga ccagctgcgg tattgttatc tgcgagaatc ggttcaagtt gtccgctcag
    36721 cgtcatgcgt tcctgaactt cacttaacag ttgttcgcgt tcttcttcta atagctgttg
    36781 atttaacgtc gttgcttgca cgatcagact taaacgccaa cgctggagga aaagcgtatg
    36841 taacgctgaa gtgatcgtgc tgttagcatc aaccaactgt cgggcttgtt ctgcccatgg
    36901 ggaattcaga cgatgcagta agtccaggat ctgtggtagc tgcacaataa actgcggcgt
    36961 ggagaggagc tggctttgct gatagcacat cacctcttcg gtgagttccg gcgggactcg
    37021 ggcatctttc agccgactgc gcagcgcctc acgccagcgg ggaacatcat cagtgattgc
    37081 cgccttcagt cgtgggaatt tttcaaagaa gactgccagc tgcggtgagg ccagcagcgc
    37141 gatgatcatc tcttcgatca atccctcttc gctgacggcc agcatcacat taagcgtatc
    37201 cagcgttagc attgttgtgc ctggcgaatc tgtgcgccga catcctgtag gctggcttca
    37261 atgcgaccta accagtcgcc agggataaac agacattttt gctgttcgct gaataaagcg
    37321 tgttgcttgc gccagtcact ttcaagctct tccagttgtt gttttatttc acctggcaga
    37381 ccttcagccg atgaaccggg aagtgccagc gtactgcctt gtaaacttac atcgcgtaca
    37441 acaagatgtt gggcgctatc cacttccaga ttcaatttct gggcaaagcc gataccgttc
    37501 agtttgccgc gaatttcacc acctttactc agccactgtt ccagcgcgtt acgctcaaag
    37561 gagatatgaa ccacttccat atcatgcaat tttaacggtt tttgcagcag cagagtcaga
    37621 gtggaagcag taacgttaac aggaagttga tactgctgac gacggctgaa aatgccgccc
    37681 agacgaatta ccgttaaggc tgttttatcg ctttgttgct gctggagttg caggtgacgt
    37741 tgcacaatcg cgccaaggcg agtcaacatc ccttgctgtt gccaggcgtg accggtcatc
    37801 aatacatcaa tttgttgttg tatcaaattc aggctttgcg cgtcatacca caggcaatct
    37861 ttcagcaaaa tgagatctac cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc
    37921 tgcaataagc ggatcgcttt tttccagcga cgatccgaga cataaggcgc atccggtaat
    37981 ttatccagtt gctggcgcag cataaaaatc agctcaaata catgatcggg cagcgtaatt
    38041 tcaccaatct ctttctgcca gcgttcatat tcttcatctg tgacctgcaa ggcatcagga
    38101 accggattgt cgttttcatc ctgttgactg gtcagcatgg agcggaaatt cgctttatcc
    38161 tgcactttat ctaaccacag acgaatcagc atgcggtcat ataacgcttc cagactgctg
    38221 tctgcttccg gcagctcgtt ggaggccgcc accagcagac gcatcgggat tttttctacg
    38281 tgtgcaccgt tgcggaactg gcgctcgtta atggcggtga gcaaggtatt aagaattgcc
    38341 gggcccgctt tccagatctc atccagaaag acaatttctg cttccggcag gtaaccgctg
    38401 gttaaacgtt catagcgccc ttcatctttt agcgcctgaa tagaaagggg accaaaaact
    38461 tcttccggcg tggagaagcg ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg
    38521 aattttaagc gccgggcgat caaacttttg gcaatacctg gcgggccaag gaggaacaca
    38581 ctttcaccac ttaatgccgc taataaacac aagcggatgg cgtggctacg ttcataaagc
    38641 cccttttcca acgaactgct caggcgggaa attctttccg ctaataaatg agggtgagcc
    38701 ataatgaagt ggcgtccttt cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct
    38761 tgctggtggc taaaatagtc tcaaaggggg ggtatttttc tttgagccag gttaatgtgg
    38821 ccgcatttag gagtacgatt ttgccgttaa tcgtgcatac tgtgcgcttt tttgtgggcc
    38881 aagggactaa gcacacattt catatttcaa cgaaagacta gtctatgagc actgataata
    38941 agcaatcatt gcccgcgatt accctcgcgg cgattggagt tgtctacggc gatattggta
    39001 ccagcccgtt atatacactt cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac
    39061 gcgatgccgt gtttggcttt ttatcgctga tcttctggct gctaatcttt gtggtttcca
    39121 ttaaatatct caccttcgtg atgcgggcag ataacgccgg tgaggggggg atcctgacgt
    39181 tgatgtcgct tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg
    39241 ggctaatcgg cggcagcttt ttctatggtg aagtcgtcat aacacccgct atttcggtga
    39301 tgtcagccat tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg atagttcccc
    39361 tctcaattat cgttctcact ttattattta tgattcaaaa acatggcacg gctatggtcg
    39421 gtaagctgtt tgcgccgatc atgctgacct ggtttttgat tctggcagga ctggggttac
    39481 gtagcattat tgctaacccg gaagtgctgc atgcgctgaa tccaatgtgg gcggtgcatt
    39541 tcttccttga atacaaaacg gtttctttta ttgcattagg ggcagtggtg ttgtcgatta
    39601 cgggggtcga ggcgctgtat gctgatatgg ggcactttgg taagttccct attcgcctgg
    39661 cgtggttcac cgtcgtatta ccttccttga cccttaatta cttcggtcag ggagcgctgt
    39721 tgttaaagaa cccggaagcg attaagaacc cgttcttcct gttggcaccg gactgggcgc
    39781 tgatcccgct gctgatcatc gccgcactgg cgacggtaat tgcctcgcag gcggttatct
    39841 ctggcgtctt ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg ccgatgcgca
    39901 ttattcacac ctccgaaatg gagtcagggc aaatctatat tccgtttgtg aactggatgc
    39961 tctatgtcgc ggtcgtgatt gtgattgtca gctttgagca ctccagcaac ctggcggcgg
    40021 cgtacgggat tgcggtgacc ggaaccatgg tgctgacgtc tattctctcg actaccgtgg
    40081 cacgtcagaa ctggcactgg aataagtatt ttgttgcgct gatcctgatt gctttccttt
    40141 gtgtcgatat tccattgttc accgctaacc tcgataaact gctctccggc ggctggttgc
    40201 cattgagcct cggtactgtg atgtttatcg tgatgaccac ctggaagagc gagcgtttcc
    40261 gcttgctgcg gcggatgcat gaacatggta actctctgga agcgatgatt gcttcgctgg
    40321 agaaatcacc gcctgttcgc gtgcccggga ccgcggtgta tatgtcgcgt gcaatcaacg
    40381 tcattccctt tgcgctgatg cataacctta aacataacaa ggtattgcat gagcgggtga
    40441 ttctgttaac tctgcgtacc gaagacgcgc catatgtcca taatgtccgt cgggtacaga
    40501 ttgaacaact gtcgcccact ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc
    40561 caaacgtgga agaagttttc caccgctgcg gtctggaagg attaagttgc cggatgatgg
    40621 aaacctcctt ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg tggtatttgc
    40681 gtctgcgcgg caagctgtac ttgctgctgc aacgtaatgc gctgcgtgca ccagatcaat
    40741 ttgaaattcc gccaaacagg gttatcgaac tgggtactca ggtcgaaatc taacgccaga
    40801 cgcctccttt ctccataagg gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt
    40861 ttcgaggttg atcacatttc cgtaacgtca cgatggtttt cccaactcag tcaggattaa
    40921 actgcgggtc agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt
    40981 aattctgata tttcatcggt gatctcccgt ctgggacata ccgatacgct ggtggtgtgt
    41041 gatgctggtt tacccatccc caaaagtaca acgcgtatcg atatggcatt aacccagggt
    41101 gtaccttctt ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgcc
    41161 attatcgcgg aagagatcaa acaacataat ccgcaactcc acgaaacgtt gctcactcac
    41221 cttgagcagc tgcaaaaaca ccagggaaat accattgaaa ttcgttacac cacgcatgaa
    41281 caattcaaac aacaaaccgc agaaagtcag gcggtaattc gcagcggaga atgttctccg
    41341 tatgcgaata tcattctctg tgctggcgtg acgttctgag gccgtcatgg aagcattact
    41401 tcagcttaaa ggcatcgata aagccttccc gggcgtaaaa gccctctcgg gcgcagcgtt
    41461 aaatgtctat ccgggccgcg tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac
    41521 catgatgaaa gtgcttactg gtatctatgc tcgcgatgcc ggcacgcttt tatggctggg
    41581 gaaagaaacg acatttaccg ggccgaaatc ttcccaggaa gccgggattg ggattatcca
    41641 tcaggaactg aacctgatcc cgcagttgac cattgccgaa aacattttcc tcggtcgtga
    41701 gtttgttaat cgctttggca aaattgactg gaaaaccatg tatgccgaag cggataaatt
    41761 gctggctaaa cttaacctgc gctttaaaag cgacaagctg gtgggcgatc tttccatcgg
    41821 tgaccagcaa atggttgaaa tcgccaaagt gctgagcttt gagtcgaaag tcatcattat
    41881 ggatgaaccg accgatgcgc tgaccgatac cgaaaccgaa tccctgttcc gcgtcatacg
    41941 cgagctgaaa tcgcaaggcc gcggtattgt ctatatctcc caccgcatga aagaaatctt
    42001 cgagatttgc gatgacgtta ccgtttttcg tgacgggcaa tttattgctg agcgcgaagt
    42061 ggcatcactg accgaagatt cgctgattga gatgatggtg ggccgcaagc tagaagatca
    42121 atatccacac ctggacaaag cgccgggaga tatccgcctg aaagtcgata atctctgcgg
    42181 acctggcgtt aacgatgtct cttttacttt acgcaaaggc gaaattcttg gcgtctctgg
    42241 tttgatgggc gcaggtcgta ccgaactgat gaaagtgctc tacggcgcac taccgcgcac
    42301 cagcggttac gtcaccctgg atgggcatga agtcgttacc cgttcaccgc aggatggcct
    42361 ggcaagcggc attgtgtata tctccgaaga ccgtaaacgt gacggtttag tgttgggcat
    42421 gtcagtaaaa gagaacatgt cgctgacagc gctgcgctac ttcagccgcg ctggcggcag
    42481 tttgaagcat gccgatgaac agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa
    42541 aacaccgtcg atggaacagg caattggtct gctttccggt ggcaatcagc aaaaagtggc
    42601 gattgcccgc ggtctgatga cacgccccaa agtgttgatc cttgatgaac ctacccgtgg
    42661 cgtagatgtc ggcgcgaaaa aagagatcta tcaactgatt aaccagttca aagccgatgg
    42721 cttgagcatc attctggtgt catcggagat gccagaagta ttaggcatga gcgatcgcat
    42781 catcgtcatg catgaagggc atctcagcgg ggaatttact cgtgagcagg ccacccagga
    42841 agtgttaatg gctgccgctg tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga
    42901 caacccagac tgtctctggt cgccgttatt tcacgaaagc gtggctgatg gagcagaaat
    42961 cgcttatcgc tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg aactttttca
    43021 ccatcaataa cttattcaat attctccagc aaacctcggt gaacgccatt atggcggtcg
    43081 ggatgacgct ggtgatcctg acgtcgggca tcgacttgtc tgtaggttct ctgttggcgc
    43141 taaccggcgc agttgctgca tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg
    43201 ctgc
//
LOCUS       DAFATA010000025        42264 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFATA010000025 DAFATA010000000
VERSION     DAFATA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.025"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1413
                     /gene="msbA"
                     /locus_tag="LGJ20_001830"
     CDS             <1..1413
                     /gene="msbA"
                     /locus_tag="LGJ20_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBK2385899.1"
                     /translation="VSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGL
                     FIMMFYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGH
                     KEVLIFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASF
                     PSVMDSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDE
                     GKRVIERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASL
                     ITRFYDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYS
                     REQIEEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILIL
                     DEATSALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTH
                     NDLLEHRGVYAQLHKMQFGQ"
     gene            1410..2396
                     /gene="lpxK"
                     /locus_tag="LGJ20_001831"
     CDS             1410..2396
                     /gene="lpxK"
                     /locus_tag="LGJ20_001831"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBK2385900.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            2433..3665
                     /locus_tag="LGJ20_001832"
     CDS             2433..3665
                     /locus_tag="LGJ20_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBK2385901.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            3717..3899
                     /gene="ycaR"
                     /locus_tag="LGJ20_001833"
     CDS             3717..3899
                     /gene="ycaR"
                     /locus_tag="LGJ20_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBK2385902.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            3896..4642
                     /gene="kdsB"
                     /locus_tag="LGJ20_001834"
     CDS             3896..4642
                     /gene="kdsB"
                     /locus_tag="LGJ20_001834"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBK2385903.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            4796..5689
                     /locus_tag="LGJ20_001835"
     CDS             4796..5689
                     /locus_tag="LGJ20_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBK2385904.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(5666..6445)
                     /gene="elyC"
                     /locus_tag="LGJ20_001836"
     CDS             complement(5666..6445)
                     /gene="elyC"
                     /locus_tag="LGJ20_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBK2385905.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPHEQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            6581..7366
                     /gene="cmoM"
                     /locus_tag="LGJ20_001837"
     CDS             6581..7366
                     /gene="cmoM"
                     /locus_tag="LGJ20_001837"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBK2385906.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7363..8685
                     /gene="mukF"
                     /locus_tag="LGJ20_001838"
     CDS             7363..8685
                     /gene="mukF"
                     /locus_tag="LGJ20_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBK2385907.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            8666..9370
                     /gene="mukE"
                     /locus_tag="LGJ20_001839"
     CDS             8666..9370
                     /gene="mukE"
                     /locus_tag="LGJ20_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBK2385908.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            9370..13830
                     /gene="mukB"
                     /locus_tag="LGJ20_001840"
     CDS             9370..13830
                     /gene="mukB"
                     /locus_tag="LGJ20_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBK2385909.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            14091..15938
                     /gene="ldtD"
                     /locus_tag="LGJ20_001841"
     CDS             14091..15938
                     /gene="ldtD"
                     /locus_tag="LGJ20_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBK2385910.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            16119..16667
                     /locus_tag="LGJ20_001842"
     CDS             16119..16667
                     /locus_tag="LGJ20_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBK2385911.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            16694..17341
                     /gene="gloC"
                     /locus_tag="LGJ20_001843"
     CDS             16694..17341
                     /gene="gloC"
                     /locus_tag="LGJ20_001843"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBK2385912.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(17563..18753)
                     /gene="aspC"
                     /locus_tag="LGJ20_001844"
     CDS             complement(17563..18753)
                     /gene="aspC"
                     /locus_tag="LGJ20_001844"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBK2385913.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(18938..20026)
                     /gene="ompF"
                     /locus_tag="LGJ20_001845"
     CDS             complement(18938..20026)
                     /gene="ompF"
                     /locus_tag="LGJ20_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBK2385914.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(20628..22028)
                     /gene="asnS"
                     /locus_tag="LGJ20_001846"
     CDS             complement(20628..22028)
                     /gene="asnS"
                     /locus_tag="LGJ20_001846"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK2385915.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(22197..23399)
                     /gene="pncB"
                     /locus_tag="LGJ20_001847"
     CDS             complement(22197..23399)
                     /gene="pncB"
                     /locus_tag="LGJ20_001847"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK2385916.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            23665..26277
                     /gene="pepN"
                     /locus_tag="LGJ20_001848"
     CDS             23665..26277
                     /gene="pepN"
                     /locus_tag="LGJ20_001848"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBK2385917.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(26484..27251)
                     /gene="ssuB"
                     /locus_tag="LGJ20_001849"
     CDS             complement(26484..27251)
                     /gene="ssuB"
                     /locus_tag="LGJ20_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBK2385918.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(27248..28039)
                     /gene="ssuC"
                     /locus_tag="LGJ20_001850"
     CDS             complement(27248..28039)
                     /gene="ssuC"
                     /locus_tag="LGJ20_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBK2385919.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(28050..29195)
                     /gene="ssuD"
                     /locus_tag="LGJ20_001851"
     CDS             complement(28050..29195)
                     /gene="ssuD"
                     /locus_tag="LGJ20_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBK2385920.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(29192..30151)
                     /gene="ssuA"
                     /locus_tag="LGJ20_001852"
     CDS             complement(29192..30151)
                     /gene="ssuA"
                     /locus_tag="LGJ20_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBK2385921.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEIAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(30144..30719)
                     /gene="ssuE"
                     /locus_tag="LGJ20_001853"
     CDS             complement(30144..30719)
                     /gene="ssuE"
                     /locus_tag="LGJ20_001853"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBK2385922.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            31075..31617
                     /locus_tag="LGJ20_001854"
     CDS             31075..31617
                     /locus_tag="LGJ20_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2385923.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            31700..32401
                     /locus_tag="LGJ20_001855"
     CDS             31700..32401
                     /locus_tag="LGJ20_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2385924.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            32426..35026
                     /locus_tag="LGJ20_001856"
     CDS             32426..35026
                     /locus_tag="LGJ20_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBK2385925.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            35017..36087
                     /locus_tag="LGJ20_001857"
     CDS             35017..36087
                     /locus_tag="LGJ20_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2385926.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKSSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            36099..36641
                     /locus_tag="LGJ20_001858"
     CDS             36099..36641
                     /locus_tag="LGJ20_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2385927.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            36649..37164
                     /locus_tag="LGJ20_001859"
     CDS             36649..37164
                     /locus_tag="LGJ20_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2385928.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            37130..37867
                     /locus_tag="LGJ20_001860"
     CDS             37130..37867
                     /locus_tag="LGJ20_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBK2385929.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADKESIAILLTNTSQESWLINSKINRPTRWAGGEVSTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            37978..38988
                     /gene="pyrD"
                     /locus_tag="LGJ20_001861"
     CDS             37978..38988
                     /gene="pyrD"
                     /locus_tag="LGJ20_001861"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBK2385930.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLIENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICMD
                     KIYPYAGYIAINISSPNTPGLRTLQYGEALDDLLIAIKNKQNDLQKIHQKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGATLVQIYSGFIFKGPPLIKEIVS
                     NI"
     gene            39162..39704
                     /gene="zapC"
                     /locus_tag="LGJ20_001862"
     CDS             39162..39704
                     /gene="zapC"
                     /locus_tag="LGJ20_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBK2385931.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     NPSGFCVDDAALYFSFEEKCRDLPLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMQGPAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(39701..40810)
                     /gene="ycbX"
                     /locus_tag="LGJ20_001863"
     CDS             complement(39701..40810)
                     /gene="ycbX"
                     /locus_tag="LGJ20_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBK2385932.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADDSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            41054..>42264
                     /gene="rlmKL"
                     /locus_tag="LGJ20_001864"
     CDS             41054..>42264
                     /gene="rlmKL"
                     /locus_tag="LGJ20_001864"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBK2385933.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNL"
BASE COUNT        10501 a        10403 c        11173 g        10187 t
ORIGIN      
        1 gtttcatttt ttgacaaaca gtcaacgggg acgctgttgt cacgtatcac ctacgattcc
       61 gaacaggttg cttcttcttc ttccggcgca ctgataacgg ttgtgcgtga aggtgcgtca
      121 atcatcggcc tgttcatcat gatgttctat tacagttggc aactgtcgat cattttgatt
      181 gtgctggcac cgattgtttc gattgcgatt cgcgtagtat cgaagcgttt tcgcaacatc
      241 agtaaaaaca tgcagaacac catggggcag gtgaccacca gcgccgaaca aatgctgaag
      301 ggccacaaag aagtattgat tttcggtggt caggaagtgg aaacgaaacg ctttgataaa
      361 gtcagcaacc gaatgcgtct tcaggggatg aaaatggttt cagcctcttc catctctgat
      421 ccgatcattc agctgatcgc ctctttggcg ctggcgtttg ttctgtatgc ggcgagcttc
      481 ccaagtgtca tggatagcct gactgccggt acgattaccg ttgttttctc ttcaatgatt
      541 gcactgatgc gtccgctgaa atcgctgacc aacgttaacg cccagttcca gcgcggtatg
      601 gcggcttgtc agacgctgtt taccattctg gacagtgagc aggagaaaga tgaaggtaag
      661 cgcgtgatcg agcgtgcgac tggcgacgtg gaattccgca atgtcacctt tacttatccg
      721 ggacgtgacg tacctgcatt gcgtaacatc aacctgaaaa ttccggcagg gaagacggtt
      781 gctctggttg gacgctctgg ttcgggtaaa tcaaccatcg ccagcctgat cacgcgtttt
      841 tacgatattg atgaaggcga aatcctgatg gatggtcacg atctgcgcga gtataccctg
      901 gcgtcgttac gtaaccaggt tgctctggtg tcgcagaatg tccatctgtt taacgatacg
      961 gttgctaaca acattgctta cgcacggact gaacagtaca gccgtgagca aattgaagaa
     1021 gcggcgcgta tggcctacgc catggacttc atcaataaga tggataacgg tctcgataca
     1081 gtgattggtg aaaacggcgt gctgctctct ggcggtcagc gtcagcgtat tgctatcgct
     1141 cgagccttgc tgcgtgatag cccgattctg attctggatg aagctacctc ggctctggat
     1201 accgaatccg aacgtgcgat tcaggcggca ctggatgagt tgcagaaaaa ccgtacctct
     1261 ctggtgattg cccaccgctt gtctaccatt gaaaaggcag acgaaatcgt ggtcgtcgag
     1321 gatggtgtca ttgtggaacg cggtacgcat aacgatttgc ttgagcaccg cggcgtttac
     1381 gcgcaacttc acaaaatgca gtttggccaa tgatcgaaaa aatctggtct ggtgaatccc
     1441 ctttgtggcg gctattgctg ccactctcct ggttgtatgg cctggtaagt ggcgcgatcc
     1501 gtctttgcta taaactaaaa ctgaagcgcg cctggcgtgc ccccgtaccg gttgtcgtgg
     1561 ttggtaatct caccgcaggc ggcaacggaa aaaccccggt cgttgtctgg ctggtggaac
     1621 agttgcaaca gcgcggtatt cgcgtggggg tcgtatcgcg gggatatggt ggtaaggctg
     1681 aatcttatcc gctgttattg tcggcagata ccacaacagc acaggcgggt gatgaacctg
     1741 tgttgattta tcaacgcact gatgcgcctg ttgcggtttc tcccgttcgt tctgatgcgg
     1801 taaaagccat tctggcgcaa caccctgatg tgcagatcat cgtaaccgac gacggtttac
     1861 agcattaccg tctggcgcgt gatgtggaaa ttgtcgttat tgatggtgtg cgtcgctttg
     1921 gcaatggctg gtggttgccg gcggggccaa tgcgtgagcg agcggggcgc ttaaagtcgg
     1981 ttgatgcggt aatcgtcaac ggtggtgtcc ctcgcagcgg tgaaatcccc atgcatctgc
     2041 tgccgggtca ggcggtgaat ttacgtaccg gtacgcgttg tgacgttgct cagcttgaac
     2101 atgtagtggc gatggcgggg attgggcatc cgccgcgctt ttttgccacg ctgaagatgt
     2161 gtggcgtaca accggaaaaa tgtgtaccgc tggccgatca tcagtctttg aaccatgcgg
     2221 atgtcagtgc gttggtaagc gccgggcaaa cgctggtaat gactgaaaaa gatgcggtga
     2281 aatgccgggc ctttgcagaa gaaaattggt ggtatttgcc tgtagacgca cagctttcag
     2341 gtgatgaacc agcgaaactg cttacgcaac taacctcgct ggcttctggc aactagttac
     2401 gccgcggcag cgttcgattg atggagtcat gaatgtcgct gccgcacctc tcccttgctg
     2461 atgcgcgtaa tcttcacctt gccgcacaag gcctgttaaa caaaccccgc cgtcaagcgt
     2521 cgttggagga tattccggca acgatctccc gcatgtcctt gctgcaaatc gataccatca
     2581 atattgttgc ccgtagtcca tatctggtgc ttttcagtcg tctgggaaat tatcctgccc
     2641 agtggctgga tgagtctctg gcgcgtggcg aattaatgga atactgggcg catgaagcct
     2701 gctttatgcc gcgtagcgac tttcgtctta ttcgccaccg catgctggca cctgaaaaaa
     2761 tgggctggaa atacaaagac gcctggatgc aggaacatga ggcggaaatt gcacagttaa
     2821 ttcagcatat tcatgataag gggccggtac gttcagccga ttttgagcat ccccgtaaag
     2881 gtgcaagcgg ctggtgggaa tggaagccgc ataaacggca tctggaaggt ttatttactg
     2941 ccggaaaggt gatggtgatt gaacggcgca acttccagcg cgtttatgat ttaacccacc
     3001 gtgtcatgcc tgactgggat gatgagcgcg atctcgtttc gcaaacagaa gcagaaatca
     3061 tcatgctgga taacagtgcg cgtagcctgg gaatattccg cgaacagtgg ctggcagatt
     3121 actatcggct gaaacgtccg gcactggcgg cgtggcgcga agcgagggct gaacagcagc
     3181 aaatcattgc tgtgcatgtt gaaaaattgg gcaatctttg gctgcatgct gatttgctgc
     3241 cgctactcga gcgtgcgctg gccggaaagc tcactgcaac gcacagcgcg gtactttcgc
     3301 cttttgatcc tgttgtctgg gatcgcaaac gcgcagagca gctttttgat tttagctacc
     3361 ggctggagtg ctatacccca gcgccgaaac gccagtatgg ctattttgtt ctgccattat
     3421 tacatcgtgg gcaattagtt gggcgaatgg atgccaaaat gcatcgccag acaggcatcc
     3481 ttgaagttat ctctctgtgg ttacaggaag gcattaaacc aacgacaatg ctgcaaaaag
     3541 ggttacgtca ggcgattact gatttcgcga gctggcagca ggcaacgcgg gtgacattag
     3601 gacgctgccc gcaaggtctc tttactgatt gccgcactgg ctgggaaata gaccccgtcg
     3661 cataaatgat tatgataaga tttatgcatt cataacccga tccatctgga ggaactatgg
     3721 atcatcgtct gcttgaaatc attgcctgcc cggtttgcaa cggaaaactt tggtataacc
     3781 aagaaaaaca agaacttatt tgcaaactgg acaacctggc tttcccgctg cgtgatggca
     3841 ttccagtgtt gctggaaacg gaagcccgcg tgctgactgc tgatgagagt aaatcatgag
     3901 ttttgtggtc attattcccg cgcgctacgc gtcaacgcgt ctgcccggta aaccattggt
     3961 tgatattaat ggcaaaccca tgattgttca tgttcttgaa cgtgctcgcg aatcaggtgc
     4021 cgatcgcatc atcgtggcaa ccgatcatga ggatgtcgcc cgcgccgttg aagccgctgg
     4081 cggtgaagta tgtatgacgc gcgccgatca ccagtcaggg acagaacgcc tggcggaagt
     4141 tgtcgaaaaa tgcgcattca gcgacgacac ggtgatcgtt aatgtgcagg gtgatgaacc
     4201 gatgatccct gcgacaatca ttcgtcaggt tgctgataac ctcgctcagc gtcaggtggg
     4261 tatggcgact ctggcggtgc caatccacaa tgcggaagaa gcgtttaacc cgaatgcggt
     4321 gaaagtggtt ctcgacgctg aagggtatgc actgtacttc tctcgcgcca ccattccttg
     4381 ggatcgtgat cgttttgcag aaggccttga aaccgttggc gataacttcc tgcgtcatct
     4441 tggtatttat ggctaccgtg caggctttat ccgtcgttac gtcaactggc agccaagtcc
     4501 gttagaacac atcgaaatgt tagagcagct tcgtgttctg tggtacggcg aaaaaatcca
     4561 tgttgctgtt gctcaggaag ttcctggcac aggtgtggat acccctgaag atcttgagcg
     4621 cgttcgcgct gaaatgcgct aatttcactt cacgacactt cagccaattt tgggaggagt
     4681 gtcgtaccgt tacgattttc ctcaattttt cttttcaaca attgatctca ttcaggtgac
     4741 atcttttata ttggcgctca ttatgaaagc agtagctttt atgagggtaa tctgaatgga
     4801 acagctgcgt gccgaattaa gccatttact gggcgaaaaa ctcagtcgta ttgagtgcgt
     4861 caatgaaaaa gcggatacgg cgttgtgggc tttgtatgac agccagggaa acccaatgcc
     4921 gttaatggca agaagcttta gtacgcccgg aaaagcccga caactggcat ggaaaaccac
     4981 catgctggca agaagtggga ctgtccgtat gccgactatt tatggtgtga tgacgcatga
     5041 agaacacccc ggccccgatg tcctgctact ggagcggatg cgtggtgttt cggtggaggc
     5101 accagcccga acaccagaac gctgggaaca actcaaagac caaatcgttg aagccttact
     5161 ggcctggcac cgtcaggaca gtcgcggttg cgtcggcgcg gtcgacaata ctcaggaaaa
     5221 tttctggccc tcatggtacc ggcaacatgt tgaagtgcta tggaccacgc tcaatcagtt
     5281 caataacacc ggtctgacga tgcaggataa gcggatcctg tttcgcactc gcgaatgtct
     5341 cccggcatta tttgaaggct ttaacgacaa ttgtgtgctg attcacggta acttctgttt
     5401 acgcagcatg ttgaaagatt cgcgcagcga tcagttactg gcgatggtcg ggccgggact
     5461 aatgctttgg gcaccgcgag aatacgaact gttccgacta atggataatt ctctggcgga
     5521 agatttgctc tggagttacc tgcaacgcgc gccagtggcg gagtcgttca tctggcggcg
     5581 ttggttgtat gtgttatggg atgaagttgc gcaactggtt aataccggac gatttagtcg
     5641 gcgcaacttc gatctggcat caaaatcact cttgccgtgg ctcgcctgac gaacctttta
     5701 gccactgcca gatacggcca agcgtttcat agccaacacg atcgctatgc atcaaccaga
     5761 ccggagaagg gattgcccgt tcccacgggt taagcggcga gtcgatggcc aactgattcg
     5821 ccggggctgg tagcggattt aacccttcct gctgaaaaaa gatcatcgcc cgcggcagat
     5881 gtgatgcgga ggtcaccagc aggaaagggg cgtcaccaat cgcctgtttc actgccgcag
     5941 cttcttcttc ggtatctttt ggcaaatcca gggtgataat ttgctcgtgc ggcacgccca
     6001 gcgattgcgc aactctggca cctacttccg ctgtactcac cgtattggtt tttgctacgc
     6061 ctcccgtgaa gatcagtttt gatcccggat tttcgcgcca taagcgaata ccttcattca
     6121 ggcgtggcag actgttattg attaaattag agctcggtgc ccactgtggg ttccaggtat
     6181 aaccacctcc gagcaccacg atatagtcca ctttttgcga attattccat gtcggatagg
     6241 tgctttcgat gggacgcagc aggcggtcgg ctaccggttg caaactcaat aacaagagtg
     6301 ccagccaccc aatgctaata aaaatcttcc cggttttttg aaaacggcta aaccacagca
     6361 gcgccaggcc agcgccaata attaacaaca tcagcggaag tggcagcaac atattgccaa
     6421 tcactttttt cagtgtaaaa agcatccttt ctggttcctt ttttaaccat atagcaaggg
     6481 atcgccagcg atattacacc agacgggttc attctcgccg tggctgtgac aaaatagcgt
     6541 ctttatcaga aaacgtctta gccaaccggt ggagaaagca atgcaggatc gcaattttga
     6601 tgatattgcg gaaaagtttt cccgtaacat ttacggcacc accaaagggc agcttcgaca
     6661 ggctattctg tggcaggatc tcgatcacgt gctggcggaa atgggcccgc aaaaactgcg
     6721 tgtgctggat gctggcggtg gtgaagggca gaccgcaatc aaaatggccg agcgtgggca
     6781 tcaggtcatt ttatgcgatc tttctgcgca gatgatcgac cgcgcaaaac aggcggcaga
     6841 agcaaaaggt gtgagcgaca acatgcaatt tatacattgc gccgctcagg atgttgcttc
     6901 gcatttggaa acgcccgttg atctgatatt gttccatgcg gtgctcgagt gggtggccga
     6961 tccccgcagt gtattgcaga ccctgtggtc agtgttgcgt ccaggcggcg tgttgtcgtt
     7021 aatgttctac aatgcgcatg gtttgttgat gcataacatg gtcgccggga attttgatta
     7081 cgtgcaggcg ggaatgccga aaaagaaaaa acggacgctt tcgccagatt atccacgcga
     7141 cccggcgcag gtttatctgt ggctggaaga agctggttgg caaattatgg gtaagacagg
     7201 cgttcgcgtg tttcatgatt atctgcgcga gaagcaccag cagcgcgact gctatgaagc
     7261 attacttgaa ttagaaacgc gttattgccg tcaggaaccg tatattaccc tggggcgtta
     7321 tattcatgtc accgcgcgca aaccgcagag caaggataaa gtatgagtga attttcccag
     7381 acagtccccg aactggttgc ctgggccaga aaaaatgact tctccatctc gctgccggta
     7441 gaccgactct cttttctgct ggcggttgcc acgctgaacg gcgagcgtct ggatggtgag
     7501 atgagtgaag gcgagctggt ggatgcattc cgccatgtga gtgatgcgtt tgagcaaacc
     7561 agcgaaacca tcggcgtgcg cgccaataac gcgatcaacg acatggtgcg tcaacgtctg
     7621 ctgaaccgct ttaccagcga gcaggcggaa gggaacgcaa tttaccgtct gacgccgctc
     7681 ggcatcggca ttactgacta ctacatccgt cagcgcgagt tttctacgct gcgtctttct
     7741 atgcagttgt cgattgtggc gggtgagctc aaacgcgcag cagatgccgc cgaagagggc
     7801 ggtgatgaat ttcactggca ccgtaatgtc tatgcgccac tgaaatattc ggtagcagaa
     7861 attttcgaca gtatcgacct gacgcaacgt ctgatggacg aacagcagca gcaggtgaag
     7921 gacgatatcg cccagttgct gaacaaagac tggcgggcgg cgatttccag ctgtgaattg
     7981 ttgctttcgg aaacttccgg gacgctgcgt gaattgcagg atacgctgga agcggcaggc
     8041 gacaaattgc aggctaatct gttgcgcatt caggatgcga cgatgaccca tgacgatctg
     8101 catttcgtcg atcgtctggt gttcgatctg cagagcaaac tcgatcgtat tatcagttgg
     8161 ggccagcaat ccatcgactt gtggattggc tacgaccgcc acgtacacaa atttattcgt
     8221 accgcgatcg atatggataa aaaccgcgtc tttgctcagc ggttacgtca gtcggtacaa
     8281 acctattttg atgagccgtg ggcgctaact tatgccaatg ccgatcgtct gctggatatg
     8341 cgtgacgaag agatggcact gcgcgatgaa gaagtgactg gggaacttcc tgaggatctg
     8401 gaatacgaag agtttaacga gatccgcgaa cagctggcgg cgatcatcga agaacaactt
     8461 gccgtgtaca aaaccagaca agtgccgctg gatcttggtc tggtggtacg cgaatatctg
     8521 tcacagtatc cgcgtgcacg tcactttgac gttgcgcgta ttgttattga tcaggcggta
     8581 cgtcttggcg tagcgcaagc agatttcacc ggactgccag cgaaatggca gccgattaat
     8641 gattacggag ccaaggtaca ggcgcatgtc atcgacaaat attgaacaag tgatgccggt
     8701 taagctggcg caggcgctgg cgaatccgtt atttccggcg ctggacagcg ccttacgttc
     8761 aggccgccat attggcctcg acgaactgga taatcatgca ttcctgatgg attttcagga
     8821 atatctggaa gagttttacg cgcgttataa cgttgagctt attcgcgcac cagaagggtt
     8881 cttctattta cgcccacgtt ccaccacgct gatccctcgt tccgtcttgt cggaactgga
     8941 tatgatggtc gggaaaatcc tctgttatct ctatctcagc ccggaacggc tggcgaatga
     9001 ggggattttc acccagcagg aactgtacga cgaactgctc accctggccg atgaagcaaa
     9061 actgctgaaa ctggtgaaca accgttcaac cggttcagac gttgaccgtc agaagttgca
     9121 ggagaaagta cgttcttcgc tcaaccgtct gcgtcgttta ggcatggtgt ggtttatggg
     9181 ccacgacagc agcaagtttc gcattaccga atcggtgttc cgcttcggag ccgatgtgcg
     9241 tgctggcgac gatccccgtg aagcacagcg tcgcctgatt cgtgatggcg aagcaatgcc
     9301 gattgaaaat catctgcaac tcaacgatga aaccgaagaa aatcagccag atagcggaga
     9361 ggaagaataa tgattgaacg cggtaaattt cgctcactga cgctgattaa ctggaacggc
     9421 ttttttgccc gaacttttga ccttgacgag ctggtcacga cgctttctgg cggtaacggg
     9481 gcgggtaaat ccaccaccat ggcggcgttc gttacggcgc tgatccccga cctgaccctg
     9541 ctgcatttcc gtaacactac ggaagccggg gccaccagcg gttcgcgcga taaaggtctg
     9601 cacggtaagc tgaaagcggg tgtctgttat tcgatgctcg acaccattaa ctcgcgccac
     9661 cagcgcgtgg tggtcggtgt gcgtctgcaa caggttgccg gacgcgatcg taaagtggat
     9721 atcaagccgt ttgccattca gggactgccg atgtcggtgc agccgacaca gctggtgacc
     9781 gaaaccctga acgaacgcca ggcgcgcgtg ctgccgctta acgagctgaa agacaagctc
     9841 gaggcgatgg aaggcgtgca gtttaaacag ttcaactcca ttactgatta ccactcgctg
     9901 atgttcgatc tgggcatcat cgcgcgtcgt ctgcgctccg catctgaccg tagcaaattc
     9961 tatcgtctga tcgaagcttc gctgtatggc gggatctcca gtgccattac ccgttctctg
    10021 cgcgactacc tgttgccgga aaacagcggc gtgcgtaaag cgttccagga catggaagcg
    10081 gcgctgcgtg aaaaccgtat gacgctggaa gcgattcgtg tcacccagtc ggaccgtgac
    10141 ctgtttaagc atctgatcag cgaagctacc aactacgtgg cggcggacta catgcgtcac
    10201 gccaacgagc gccgtgtcca tctcgacaaa gccctggagt ttcgtcgcga gttacatact
    10261 tcgcgtcagc aactggcggc tgagcagtac aaacacgtcg atatggcgcg tgagctggca
    10321 gagcacaacg gtgccgaagg cgatctggaa gcggattatc aggcggccag cgatcacctg
    10381 aacctggtgc aaaccgcact gcgtcagcag gagaagatcg aacgctacga agcggatctc
    10441 gatgagctgc agatccgtct ggaagagcaa aacgaagtgg tggcagaagc catcgaacgc
    10501 caggaagaga acgaggctcg cgcggaagct gccgaactgg aagtggacga gctgaaaagc
    10561 cagcttgctg actaccagca ggcgctggac gtccagcaaa cgcgcgcgat ccagtataac
    10621 caggcgattg ctgcgcttaa tcgcgccaaa gaactgtgcc atctgccgga cttaaccgcc
    10681 gacagcgccg ccgaatggct ggaaaccttc caggcgaaag agctggaagc gactgaaaaa
    10741 atgctctctc ttgagcagaa aatgagcatg gcgcaaaccg cgcacagcca gtttgagcag
    10801 gcttatcagc tggtggtggc aatcaacggc ccgctggcgc gtaacgaggc gtgggatgtc
    10861 gctcgcgaac tattgcgcga aggggtcgat cagcgtcacc tggcagagca ggttcaaccg
    10921 ttgcggatgc gcttaagtga actggaacag cgtctgcgcg agcagcaaga agccgaacgc
    10981 ctgctggcag acttctgcaa acgtcagggc aagaattttg atattgatga actggaagct
    11041 ctgcatcagg agctggaagc gcgcattgcc tctctttccg atagcgtgtc taacgcccgt
    11101 gaagagcgca tggcactgcg ccaggaacag gaacagctgc agtctcgcat tcagagtttg
    11161 atgcagcgtg cgccggtttg gctggcagcg caaaacagtc tcaaccagtt gagcgagcag
    11221 tgcggtgaag agtttacctc cagccaggac gtcaccgaat atctgcaaca gttgctggag
    11281 cgtgagcgag aggcgattgt tgaacgcgat gaagtgggcg cgcgcaaaaa cgctgtcgat
    11341 gaagagatcg aacgtttaag ccagcctggc ggctctgaag atcagcgtct gaacgcgctg
    11401 gcggagcgtt ttggcggcgt gctgctgtca gaaatttatg acgacgttag cctggaagat
    11461 gcgccgtact tctcagcgct gtatggcccg tcacgccacg ccatcgtggt gccagatctg
    11521 tcacaggtaa ctgaacacct ggaaggcttg accgattgcc cggaagatct ctatctgatc
    11581 gaaggagatc cgcagtcatt cgatgacagc gtgttcagcg ttgatgagct ggaaaaagcg
    11641 gtagtggtga aaatcgccga tcgtcagtgg cgttattcac gtttcccgga agtgccgctg
    11701 tttggtcgtg ctgcgcgtga aagccgtatt gaaagcctcc atgccgagcg tgaagtgctt
    11761 tccgaacgct tcgccacgct ctcctttgat gtacagaaaa ctcagcgtct gcatcaggcg
    11821 ttcagccgct ttatcggcag tcatctggcg gttgcgtttg agtctgaccc ggaagcagaa
    11881 atccgtcaac tgaacagccg tcgcgtcgaa ctggagcggg cgttgagtaa tcatgaaaat
    11941 gataaccagc agcagcgtat tcagtttgag caggcgaaag aaggcgttac ggcgctgaac
    12001 cgcattctgc cgcgtctcaa cctgttggct gatgacagcc tggcggatcg cgtcgatgaa
    12061 atccgcgaac gtctggatga agcccaggaa gccgcgcgtt ttgttcagca gtttggcaat
    12121 caactggcga aactggaacc gatcgtttcg gtattgcaga gcgacccgga acagttcgaa
    12181 cagctaaaag aagattacgc gtactctcag cagatgcagc gcgatgcccg tcagcaggcg
    12241 tttgccctga cggaagtggt gcagcgtcgt gcgcacttta gctattctga ctcggcagaa
    12301 atgctgagcg gtaatagcga tctcaacgaa aagctgcgtg aacgtctgga acaggcggaa
    12361 gcggagcgta ctcgcgctcg tgaagcgttg cgtggtcacg cagcgcagtt gagtcagtac
    12421 aaccaggtgc tggcttcgct gaaaagttct tacgacacca aaaaagagct actcaacgat
    12481 ctgcaacgtg aattgcagga tatcggcgtg cgcgccgata gcggggcaga agagcgggcg
    12541 cgaattcgcc gtgacgagct gcatgcgcaa ctgagtaata accggtcccg ccgcaaccag
    12601 ctggaaaaag cactgacttt ctgcgaagcg gagatggaca atctgacccg caaactgcgc
    12661 aagctggagc gggattactt tgagatgcgc gagcaggtag tgaccgccaa agcgggctgg
    12721 tgtgcagtga tgcgcatggt gaaagataac ggcgttgaac ggcgcttaca ccgtcgcgag
    12781 ctggcttatc tctccgctga tgatttgcgc tctatgtcgg ataaggcatt gggtgcgctg
    12841 cgtctggcgg tggcggataa cgaacatctg cgcgacgtgc tgcgcatgtc ggaagatcca
    12901 aaacgtccgg aacgtaaaat tcagttcttc gtggcggttt atcagcatct gcgtgaacgt
    12961 attcgtcagg atattattcg taccgatgat ccggtggaag ccatcgagca gatggaaatc
    13021 gaacttagcc gactgaccga ggagttaacc tcccgcgaac agaaactggc gatcagttcc
    13081 cgcagcgtgg cgaacatcat tcgcaaaacc attcagcgcg agcagaaccg tatccgtatg
    13141 ctcaaccagg ggttgcagaa cgtatcgttt ggtcaggtga acagcgtacg tctgaacgtg
    13201 aacgtgcgtg aaacgcacgc catgctgctg gatgtgctct ctgaacagca cgagcagcat
    13261 caggatctgt ttaacagcaa ccgtctgacc ttctcggaag cgctggcgaa attgtatcag
    13321 cgtcttaatc cgcagattga tatggggcag cgcacgccgc agaccattgg cgaagaactg
    13381 ctggattacc gcaactatct ggaaatggaa gttgaggtta atcgtggttc cgatggctgg
    13441 ttgcgcgcag agtctggtgc attgtcgacc ggtgaggcga ttggtaccgg tatgtcgatt
    13501 ctggtgatgg tggtacaaag ctgggaagat gaatctcgcc gcctgcgcgg taaagatatc
    13561 tctccttgcc gcctgctgtt cctcgatgaa gcagcgcgac tggatgctcg ttctatcgcc
    13621 acgctgtttg aattgtgtga acgtttgcaa atgcagctca tcatcgcagc gccggaaaat
    13681 atcagcccgg agaaaggcac cacctataaa ctggtgcgta aagtcttcca gaataccgaa
    13741 cacgttcacg tcgtcggcct gcgaggattt gcgccgcaac tccctgaaac gcttccagga
    13801 agtgacgaag cgccttctca ggcgagttaa aattaaggcg gcagcaatgc cgccttttct
    13861 ttttccgaaa actccgtttc tgcactaaaa aagtggcaca ttacggcgcg cttttcttta
    13921 aacttcttta cattaggtta tgtaaaaacg tatcggcgtt tatatactga agataagcct
    13981 gatgagtaac aggcttgctc gtcatacttt cgtgagtatt ggcgttgtac aggcaagtcg
    14041 taaaataaca gcctggctat tcagagtatg ataaaaacag ggggcaaggg atgttgctta
    14101 atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg cctggccgta acattcgctc
    14161 cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc tggcgacagc ccggtggctg
    14221 tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac ggcaataatg gcggggatcc
    14281 agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca aatcgaatct caattacccg
    14341 caggttacaa gccggtttat cttaaccagc ttcaactgtt gtatgccgca cgcgatatgc
    14401 aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg gcagaggtgg
    14461 cgattgccgg tttccagccg cagtttaata aatgggtaga gttactgacc gatcctggtg
    14521 ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat gatgggctat ctccatttca
    14581 ttgcaaatat tccggtcaaa ggcactcgct ggctatatag cagtaaacct tatgcacttg
    14641 caacgccgcc gctctcggtg attaaccaat ggcagctggc gctggataaa ggtcaattgc
    14701 ctacgtttgt tgctggactg gcaccgcagc atccgcaata tgcggcgatg catgaatcgt
    14761 tactggcctt actcagtgac accaaaccgt ggccccaact gaccggcaaa gcaacgttgc
    14821 gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa cgcacaggca
    14881 tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact gacgcggtag
    14941 tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac aagcaaacga
    15001 catctcgtag taaacctgcg cctgccgttc gcgccgccta cgataatgaa ctggtggaag
    15061 ccgttaaacg ttttcaggca tggcaaggat tgggggcaga tggtgctatt ggcccggcaa
    15121 cacgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggct ctcaacatcc
    15181 agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac attccggcct
    15241 attcgctggt ctactatcag aacggcaatc aggtgctgga ttcgcgagtc attgtcggtc
    15301 gccccgatcg caaaacgccg atgatgagca gtgcccttaa caacgtagtg gtaaacccgc
    15361 cgtggaacgt acctccaact ctggcacgca aagatattct gccaaaagtg cgcaacgatc
    15421 cgggatatct cgaaagccat ggctatacgg tgatgcgcgg ctggaacagc agagaagcga
    15481 ttgacccatg gcaggttgac tggtctacaa tcacggcctc gaatttaccg ttccgcttcc
    15541 agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg ccgagttcag
    15601 aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat acacgcgcat
    15661 tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg ctgttgcagg
    15721 atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa gcagggtgat acacgttacg
    15781 tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt gttggtgcag
    15841 atggtcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg cgatccagct
    15901 cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc ggggaaatca
    15961 gttgtcgtaa taattagcat gattgggggc gattctctgc agcctccgtc actgctgggg
    16021 ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc gccagaagtg
    16081 catataaacg ataacattga cctgtagact tgattatcat ggacaaattc gacgctaatc
    16141 gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg ccgacccctg
    16201 cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat cttcataccg
    16261 gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa gaattggcaa
    16321 aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc gacccaggat
    16381 tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg gtgcaactca
    16441 tttccggtta tcgttctatt gataccaaca atgaactacg cgcccgcagc cgtggagtag
    16501 cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa ggtatcgcgt
    16561 taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga tattatccac
    16621 gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagtaa ttgcttaacg
    16681 aaacaggggc agtatgaact atcgtattat tccggtcacc gcattctccc agaactgttc
    16741 attaatctgg tgtgaacaaa cccgtctggc cgcactggtc gatcctggcg gcgatgcgga
    16801 aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc tgctgacgca
    16861 tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg gcgtgccggt
    16921 ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc aaagtcgtat
    16981 gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg aaggcgatac
    17041 catcagcata gggaatgtga ctttacaggt gttacattgc cctgggcata cgccgggtca
    17101 tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta ttttcaaagg
    17161 cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt cttcaatcaa
    17221 agataaattg ctgccactgg gggatgacgt tacatttatt ccgggtcacg gaccattatc
    17281 cacacttggt tatgaacgcc tgcataatcc cttcctgcaa gacgaaatgc ccgtctggta
    17341 aggcacataa aaaagcccgc ttttaatgcg ggcctggatt tctggcaaag tgcgctttgt
    17401 ttatgccgga tgcggcacga gcgccttatc cggcctacaa aatcgtgcaa attcaaaata
    17461 ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag gcaatgttgc gtttgtcatc
    17521 agtctcagcc cgcttttcag cgggcttcat tgtttttaat gcttacagca ctgccacaat
    17581 cgcttcgcac agcggagcca tgttatctgg tgtcatcccg gccacattta cgcgaccaga
    17641 agcaaccgca tatacgccaa actcttcgcg cagacgcagc acttgttctt ttgtcaggcc
    17701 actgaaggag aacatgccgt tctgtttgat gataaagctg aagtcgcggt ttgcgccttt
    17761 ttcctgcagc gtattgacga acaactgacg catacgctga atacgctggc gcatatcagt
    17821 cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc aggatggtgg caacaacaga
    17881 agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc gccgctttca tttggctgaa
    17941 tgcgcgatca acggtttcac tgtcggcagc aaccagagta caagcgccaa cacgctcgtt
    18001 gtacaggcca aagtttttag agtaggaact ggcaacaatc agctctttat gcatagccgc
    18061 gaaagcgcgc agtccttcag catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa
    18121 gtcaaacagc ggtaaccagc ctttctcaac tgagagttgt gccagtgtct gccattgttc
    18181 cagcgtaggg tcgataccgg ttgggttatg gcagcagcca tggaacagca ctacgtcgcc
    18241 agcctgagct tcattcaggc tgttaatcag tgcatcgaag tcaagagtgt gattttccgc
    18301 atcataataa gcgtattcac gaacttccag acctgcagag ttaaagacgc tcttatggtt
    18361 cggccagctt gggttgctca cccacactcg cttaacgctg gtattttttg ccaggaaatc
    18421 ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt gccgtgcgag cacgtttgtc
    18481 attgatcagg gcgctacctt taccaaacag cagttcctga gtgcagcgac caaattcagg
    18541 gatgccgtca atgccgaggt aatttttggt ggtttcattt tcgagcagat actgttcagc
    18601 ctttttcacg ctggtcagta ccggggtttt gcccgtctca tctttataga caccaatccc
    18661 gaggttaatt ttgccgggac gttcatcggc acgaaacaga tcggccaggc ccagaatcgg
    18721 gtcggcagga gcggcggtaa tgttctcaaa catgacgagg ttccattatg gttacagaag
    18781 ggaagtccgc tatcagggta acgggagatt tacaaaattc caactattac tgatgaaaac
    18841 gcaggctgtt tttgcaagac gtgagattgc tctggaaggt ataaaaaaaa caggaccaaa
    18901 gtcctgtttt ttcggcattt aacaaagagg tgtgctatta gaactggtaa acgataccca
    18961 cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc agaatcgatc tggttgatga
    19021 tgtagtcaac ataggtggac atgtttttgt tgaagtagta ggttgcgccc acttcaaagt
    19081 agttcaccag atcaacatca ccgatacctt ctacgtcttt cgctttagat ttggtgtaag
    19141 cgatggacgg acgcagaccg aaatcgaact ggtattgcgc aactaacaga acgtcttgcg
    19201 ttttgttggc gaagccgctg gtgtttgtaa atttattagt gatcggcgta gcgttacggg
    19261 tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc gtacttcaga ccagtagccc
    19321 actgttcagc ttttttaccg ttgccaagag gttgagcttc ttgcaggttg gtacggtcag
    19381 ctgcaccata agcaccaacg ataccaaagc cttcgtattc gtagctgata gaaccgccaa
    19441 caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt tttacccagg tactgaacag
    19501 cgaagttcag gccatcaacc agaccaaaga agttggagtt acgataggta gcaacgccgc
    19561 caacacgacc aacgaagaag tcatcgctgt atgcagtatc accaccaaat tctggcagca
    19621 tatcggtgta acccagtgca tcataaacca caccgtagtt acggccgtaa tcgaaagaac
    19681 caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt tttgttacca gtttgagcgt
    19741 cagcgccttc agagttgtta ccctggaagt tatattccca ctgaccataa ccggtcagat
    19801 cggaattgat ttgagtttcc cctttaaaac caagacgggc ataggtcatg tcgccattgc
    19861 caccgtaact gttttcaccg ttacccttgg aaaaataatg cagaccaaca gctttaccgt
    19921 acagatctac tttgttgcca tctttgttat agatttctgc agcgtttgca gtacctgcta
    19981 ctaacagagc agggacgatc actgccagaa tattgcgctt catcattatt tattaccctc
    20041 atggtttttt ttatgacacc tgccactgcc ttcaataagt tctgtcaata aaaatttacg
    20101 gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc attcaaacta
    20161 acgtttcgct accgtgaaag tgctacaaag ataaagattt agtttcaaaa agaaaaaata
    20221 tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg tcatatttcc atagaaacgt
    20281 gatgatattt acagcaattt ttatctattt atatgattta cttatattta aattaactaa
    20341 acggaaattt tgtttctgat ggaaacttta tcgacctggc acaaaatcct ctttcagaca
    20401 tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa actaatccgc
    20461 aataagaatg cgggttattt tttgtgcgta aatgttgcaa tgtaatattc cgtaacagga
    20521 tgatcgttat ggcggggttg caaatagatt gcttgactta acaaacagat aacttgacag
    20581 aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca gaagctggcg
    20641 ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc agttacgtaa
    20701 gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc gtagcgacgc
    20761 agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat acgctcgtcc
    20821 agaacgtcca gacgttcttc acgctgggag ccaccaatga tctcaccgat gcccggagcc
    20881 agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata gaacgcttta
    20941 atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc cgccagataa
    21001 cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa cttcctgccg
    21061 cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc ggcctcaatg
    21121 aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa tttcatgtcg
    21181 tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc agccagaccc
    21241 gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa ttccgccagg
    21301 tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata aattttggat
    21361 aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa agactcttta
    21421 ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag gttttccaga
    21481 tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc ggtaatcagt
    21541 ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc ctgcgccagc
    21601 gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag gtgagcgact
    21661 tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc tggatcttca
    21721 acccaaccag caacttcaac tttgctggcc tgaatttcaa attgttgccc ctggcccggc
    21781 gacgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg caggacgtct
    21841 tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa gcaggaaccg
    21901 tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg tacccatccg
    21961 cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc tacaggcaca
    22021 acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc cacccgtaaa
    22081 tggggggata cctatgttac ctggcatctg caatcagaca agcagaattc gcaaatgcag
    22141 cgaaagattt cggaattaaa ggtaaaaaaa gggagccgat tagctccctg atgatattaa
    22201 ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac aaacgcttta
    22261 tcgtggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt accgttacac
    22321 tcaaccaact taatcacaat attcaggggt tttacctggg ggatatcgca ggtcaggcga
    22381 gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag ctcaaccgct
    22441 ttgcgtaaat ccagattgtc agagaaaacc agagttttac tctgtggatc aattcccagc
    22501 ttttcataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga gtcatgacgc
    22561 aggccctgat agcgactggc gaactcgaca ccgaaatcac gcaggaaagc atccatagtg
    22621 atgcagtcgg ttaatgcaat gccaagttgg tcgggatatt cttccagcca ggcagcaagt
    22681 gcagctcgct ggctgttggc tagatccggg ctgatttgct gatgtgcctg gaaccattcg
    22741 tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta gttgctggtg
    22801 cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac ttcgcgagaa
    22861 aaacggcgac gggtgccaaa atccataaga tggaagcgcg acatatcaag gccggcggtt
    22921 aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc aacgtcggcc
    22981 tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg aacttcccag
    23041 aggatgactt cacgccacgg gccgcttaaa cgaatatcca gcttgccatt atcgttggat
    23101 acggtgactt gttccgggtt aaagcggaac tcgcgtaacc agttaagata gtcggcctta
    23161 aagaaaggca gagcagagag ccactgatat tcatcatcct gcaggcgcag gtgctgcatc
    23221 gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc acctcggcaa
    23281 cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg ctgcatatgc
    23341 aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa ttgtgtcata
    23401 ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa taaacatctt caggaactgc
    23461 tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc cacctttctg
    23521 gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt ttgtacctga
    23581 aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa caccagactc
    23641 tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata ccgtcacgat
    23701 tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct cgacgcgcaa
    23761 aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga tgctccgctt
    23821 cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga gccgtggacc
    23881 gcctggaaag aagaagaggg cgcactggtc atcagtaatt tgccggagcg ttttacgctt
    23941 aagatcatta atgaaataag cccggcggcg aatacggcgc tggaagggct ttatcagtca
    24001 ggcgatgcgc tttgcaccca gtgtgaagcc gaaggtttcc gccatattac gtattatctc
    24061 gaccgcccgg acgtgctggc gcgttttacc accaaaatta ttgccgataa aatcaaatat
    24121 cccttcctgc tttccaatgg taaccgcgtt gcgcaaggcg aactcgaaaa cggacgccat
    24181 tgggtacagt ggcaggaccc gttcccgaaa ccgtgctacc tgtttgcgct ggtggcaggc
    24241 gactttgatg tactgcgcga tacctttacc acgcgttctg gtcgcgaagt agcactggag
    24301 ctgtacgtcg atcgcggcaa ccttgatcgc gcgccgtggg cgatgacctc gctgaaaaac
    24361 tccatgaaat gggatgaaga acgctttggc ctggagtatg acctcgacat ctatatgatc
    24421 gtcgcggtgg atttcttcaa tatgggcgca atggagaata aggggctgaa tatctttaac
    24481 tccaaatatg tgttggcccg caccgacacc gccaccgaca aagattatct cgatattgaa
    24541 cgcgttatcg gccatgaata tttccataac tggaccggta accgagtgac ctgccgcgac
    24601 tggttccagc tcagcctgaa agaaggttta accgtcttcc gcgatcagga attcagctct
    24661 gaccttggtt cccgcgcggt aaaccgtatc aataatgtgc gcaccatgcg cggattgcag
    24721 tttgcggaag acgccagccc gatggcgcac ccgatccgcc cggatatggt cattgagatg
    24781 aacaacttct acaccctgac cgtttacgag aagggcgcgg aagtgattcg catgatccac
    24841 accctgctgg gcgaagaaaa cttccagaaa gggatgcaac tctatttcga gcgccatgat
    24901 ggcagcgcgg caacctgcga cgactttgtg caggcgatgg aagatgcgtc gaatgtcgat
    24961 ctctcccatt tccgccgttg gtacagccag tccggcacgc cgattgtgac cgtcaaagac
    25021 gactacaatc cggaaaccga gcagtacacc ctgaccatca gccagcgcac gccagccacg
    25081 ccggatcagg cagaaaaaca gccgctgcat attccatttg ccatcgaatt gtatgacaac
    25141 gaaggcaaag tgatcccgtt gcagaaaggc ggtcatccgg tgaattccgt gctgaacgtc
    25201 acccaggcgg aacagacctt tgtttttgat aatgtctact tccagccggt gcctgcgctg
    25261 ctgtgcgaat tctctgcgcc agtgaaactg gaatataagt ggagcgatca gcaactgacc
    25321 ttcctgatgc gtcatgcgcg taatgatttc tcccgttggg atgcggcgca aagtttgctg
    25381 gcaacctaca tcaagctgaa cgtcgcccgt catcagcaag ggcagccgct gtctctgccg
    25441 gtacatgtgg ctgacgcttt ccgcgcggtg ctgctcgatg agaagattga tccggcgctg
    25501 gcggcagaaa tcctgacgct gccttctgtc aatgaaatgg ctgaactgtt cgatatcatc
    25561 gacccgattg ctattgccga agtacgcgaa gcactcactc gtactctggc gactgaactg
    25621 gcggatgagc tgctggctat ttacaacgcg aattaccaga gcgagtaccg tgttgagcat
    25681 gaagatattg caaaacgcac tctgcgtaat gcctgcctgc gcttccttgc ttttggtgaa
    25741 acgcatctgg ctgacgtgct ggtgagcaag caataccatg aagcaaacaa tatgaccgat
    25801 gcgctggcag cgctttctgc ggcggttgct gcacagctgc cttgccgtga cgcgctgatg
    25861 caggagtacg acgataagtg gcatcaggac ggtctggtga tggataaatg gtttatcctg
    25921 caagccacca gcccggcggc gaatgtgctg gagacggtgc gtggtctgtt gcagcatcgc
    25981 tcatttacca tgagcaaccc gaaccgtatt cgttcgttga ttggtgcgtt tgcgggcagc
    26041 aacccggcag cgttccatgc cgaagatggc agcggttacc agttcctggt ggaaatgctt
    26101 accgacctca acagccgtaa cccgcaggta gcttcacgtc tgattgaacc gctgattcgc
    26161 ctgaaacgtt atgatgccaa acgtcaggag aaaatgcgcg cggcgctgga acagttgaaa
    26221 gggctggaaa atctctctgg cgatctgtac gagaagatca ccaaagcact ggcttgatac
    26281 ttttgagcca aaatgcctga tgcgacgctt gacgcgtctt atcaggccta caactccgtt
    26341 gaaattgctg gttttgtagg ctggataagg cgttcacgcc gcatccggca gccgtgccca
    26401 actgcctgat gcgatgcttg acgtgtctta tcaggcctac tagtccgttg aaaatgctgg
    26461 ttttgtgcgc cacatccggc atattaacca tgacgccgaa tcagttgttc agaatgccca
    26521 cgccgcatta cccgttgcaa tacttccgct tccagctccg ctaatcgcac cgaccctaac
    26581 cggcgcggac gagggatatc caccgtcaaa tccagaccaa tttttccctc ttcaattaac
    26641 agcacccggt cagccattgc taccgcttcg ctcacatcat gcgtcaccaa cagtacggta
    26701 aagccgtgct gctgccaaag tgacacaatc aaatcctgca tctcgagtcg cgttaaggcg
    26761 tccagcgccc cgagcggttc atcaagcaac aataaacccg gtcgatgaat caacgctctt
    26821 gccagcgcca cacgctgttt ctgcccgcca gaaagtgcgg caggccattc cccggcgcga
    26881 ttctccagtc ctactgcagc caacgcccga cgcgcggcat cgcgccactg gcctttaagg
    26941 cctaacccaa cgttatcaat caccgatttc cacggcagca gacgcgcatc ctgaaacatc
    27001 attcgcgtgt cttcctgaat ctcagccagc ggtgtggtgc ccgctaacac atcgcctgcg
    27061 gttggcgttt ccagacctgc cagcaggcgc agcagggtac ttttgccacc accgctgcgg
    27121 cccaccaccg ccacaaactg acctgccgga atatgtaaat ccagttggtt caggacgata
    27181 ttttccgcgt aatgtttgct tactgcattg agcaacaatg gtgtgccctg gttcagacga
    27241 gcagtattca taccgtggcc tccttcaaat gataagccgg gttccagcgc aaccagaggc
    27301 gctctaacag ctgcgcgctg acgtcagcaa gtttgccgag cagggcgtaa agaataatag
    27361 cgactaccac cacgtccgtt tgcaaaaact cccgcgcatt catcgccaga taaccaatgc
    27421 ctgaattggc agaaatggtt tcggcaacaa tcagcgtcag ccacatcagg cccaacgcaa
    27481 aacgcacgcc gaccataatt gagggcaggg caccaggcag gatcacatgg ataaacagcg
    27541 gtatgccgga taatccatag ctacgcgcca tttccaccag accgcgatcg atattacgga
    27601 tgccatgcca ggtgttgata taaatgggga atagcgtgcc gagcgccacc agaaagattt
    27661 ttgcggattc atcaatgcca aaccacaaaa tcaccagcgg gatcagcgcc agatgcggta
    27721 cgttgcgcaa catctgaatt gaggtatcca gcagccgttc tccccagcga gatagcccgc
    27781 taatcagtcc caaaatcaac cccagcgatc cgccaattga aaagccaatc agcgcccgcc
    27841 aggaactgat cgccagatgc tgccacagtt cgccgctggc ggagagcgtc cagaacgccg
    27901 tcaccacccc ttccggtgag ggcaaaatac gcgtcgacag ccagccaact gaggaggcca
    27961 gttgccacac cgccacgatg cccaccggta aaaaccaggg ggcaacgcgc aataaccact
    28021 tcttcactgg cgttgccatt atttcttcct taactttgcg cgactttacg ggggataaaa
    28081 tcattcgcca ccgcttcgcc ttgcggattc agcggctgcg gctggggaat ttccggaatg
    28141 gcgacatcca gatgtgggaa cagcaactcg ccgacccgat acgcttcttc cagatgcgga
    28201 tagcccgaaa gcacaaagct gtcgatgcca agcgcggcgt attcgttgat tcgcgcagcg
    28261 accgtaggac catcgcccac cagcgccgtt ccggcaccgc cgcgcactaa gccaacgccc
    28321 gcccataaat tggggctgat ctccagattg tcgcgcttgc cgttatgtaa cgccgccatt
    28381 cgctgttgtc ctacggaatc cgtccgggcg aatgcggcct gtgctttggc gatagtttca
    28441 tcatcaagat gcgagattaa ccgctcggcg gcctgccacg cttcgtcgtt agtttcacga
    28501 acaatcacat gcagacgaat accgaaacga attttgcgtc catgcgcggc agctttcgcc
    28561 cgcacttgtt cgatcttctc ttttactagt tctggcggtt cgccccaggt gaggtagaga
    28621 tcaacttgtt ctgccgccag ctcctgggcg acgtctgacg atccgccaaa gtaaagcgga
    28681 ggataaggct gttgaatcgg cgggaagagc agttttgctc cgcgcacatg aatatgttta
    28741 ccgttgaaat tgacggtttc ccccagcaat aagcgccgcc agacctgggt aaattccgcc
    28801 gaggcttcgt agcgctcgct atgatcaagg aacacgccgt cgcctgccag ctcttgtgga
    28861 tcgctgcctg tgaccagatt aaacaacgca cgaccatttg agagacggtc aagcgtggcg
    28921 gcctggcgag cggcaacggt aggtgaggtt acgctggggc gcagggcgac aagaaacttc
    28981 agccgctgcg tcaccgggat catcgacgcc gccaccagcc acgcatcttc acacgatcgc
    29041 cccgttggga tcagcacacc ggtatagccc agacgatccg ccgcttgcgc aatttgttgc
    29101 agataaccgt gatcaaccgg gcgtgaacct tcttccgttc ccagatagtg cccgtcaccg
    29161 tgggtcggta aaaaccagaa catattcaga ctcataattg ttttccttcc agttgagtgg
    29221 gctgccagat gcgctggcga atatcgactt ttttcggcac cagacgattt tcataaaaca
    29281 gatctgccgt ttgctgctgt aaggcggcaa tctcggcgtt aaccggtttg atggtggtag
    29341 gagggcgatg atctaagtaa gaggcaatca ccggtgccgg taagcccatc gtttttgcca
    29401 gtaaagcgat gctttgctcg cgctggctgc gggttaacgc atcggcctca ctaaaggttg
    29461 ccagtacgcc ctgaataaaa gcgccgtttt tttctgcata ggggcgagct gccagataaa
    29521 acgatccggt ttgattgaga tcggtgccgt ctttcaacac ccgcacgccg ccctgtaata
    29581 acgcagcgga gtagtaggga tcccagatag cccaggcgtc aacgttacct tgctggaacg
    29641 cggcgcgggc atcggcaggt gtcaggtaag tgggttgtat atcggtaaac ttaagtccgg
    29701 cctgacgcag tgcacgcagt aaaaggttgt gtgaactgga acctttctga aaggcaactt
    29761 tgtgaccttt aagatcggct acggttttga tcgggctgtt ttctgccacc agaatcactt
    29821 cggctttggg cttcggtggc tcgacgccca catataccaa atcagccccg gcagcctggg
    29881 caaagattgg cggaatatcc ccggtactgc cgagatcaat actgccaacg tttaacgctt
    29941 ccaacatttg cggacccgcg gggaactcca cccaggagat ttttgattgc ggatagcgtt
    30001 tttccagtaa ctggtggctt tttgccagca ccataccaat actgcctttc tgatagccta
    30061 tacgtaacgc ttcaggcgag gattctgcag caaccgcaaa agtagagacg ctaagcaatc
    30121 ccgccagcgc cagtttaatg atgttacgca tggacattac ctcgcagaga cagaaggtca
    30181 ggaacctgaa catcgcggcg gtgcaatgcc tgccagaaag tttctagcgc ggtatcaaga
    30241 cgggtttgca gatttggcgt gaactggggt ctgtgatggt aatcaatgac ttgtgagtca
    30301 tcggcaaaca cgccgtgcag gatctcctgg gctttcagtg cgcttaaaac tggtttaagg
    30361 gcataatcga ccgccagcag atgggccacg gtaccgcccg tcgccagcgg tagcaccact
    30421 ttgccttgca aagcgcgctc tggcagcagg tcgagcaggg ttttcagcgc gccggaatag
    30481 gcggctttat acacaggtgt agcgactatc agtccatcag cctgttgcag ctgttcggtg
    30541 aaggtcttga gtgccggact atcgaaacga gcataaagca gatcttccgg ggcgaagttt
    30601 tgcagattcc agtgataaac ctctacatcg aggccattta gtttttcccg cgcatattcc
    30661 agcaaggagc tggagcgaga aggaaagcga ggactacccg ccagggtgat gacacgcata
    30721 ctctctcctt ataaccaatt gttctttttt tgttaacatt gataacaatt cggtcagtct
    30781 gtcggagaga caagaaaatg ccaaatataa attttgtgta tctttttctg aaaataaact
    30841 aaagaaaggg ctttatatat cacgcatatt tatttgttgg atagtcatta gatgttgaat
    30901 caatgaatta ctcgttcagt taatatttaa atattcatga aatctataaa ttaaagattt
    30961 gtcacttatt ggatttagta tgcttgattt aaatggcggt caattattgt tattattaag
    31021 tctaatgtca tttaaaggca ttatcatatt tacgaagaga tcaggatgat gacgatgaaa
    31081 aaaagtgtat tgacggcgtt tataactgtg gtatgtgcaa cgtccagcgt tatggcagct
    31141 gatgataatg ctatcacgga tggcaaagta acatttaatg gtaaagttat tgctccagct
    31201 tgtaccctgg tagctgcgac gaaagattca gtagtcacat tgccaaatgt cagcgccacc
    31261 aagttgcaaa ctaatggagc agtctctggc gttaaaactg atgtaccaat tgccttagaa
    31321 ggctgtgatg ttactgtaac taaaaatgcg acgtttacct ttagtggtac ggctgacggc
    31381 gtgcaaccta cagcatttgc caaccaggct accaccgatg cagcgacgaa tgttgctcta
    31441 caaatgtatc tgccagatgg ttctacatcg gtaactcccg gtaccgaaac gtcgaatatt
    31501 cagttagcgg attctgctga gcaaacagtg acctttaaag ttgattatat cgctacgggg
    31561 aaagcgactt ctggtaatgt gaatgcggta acaaatttcc atattaacta ttattaatag
    31621 aactcattaa ttgttttatt aattagtacc cctctagtgt tctggagggg atattcatat
    31681 tttttaagag tgactattta tgaaaacttg cataacaaag ggaattgtga ccgtaagttt
    31741 aacggcaata ttactcagct gctcatccgc atgggcggca ggtaaaggcg ggattggact
    31801 tgcagcaaca cgtcttgttt attcagaagg tgaagagcaa atttcactgg gtgtacgtaa
    31861 caccagtccg gatgttcctt atcttattca gtcatgggtg atgaccccag ataataaaaa
    31921 atcagcagat tttattatta caccaccgct atttgtgctg aatccggcta atgagaatct
    31981 gttacgcatt atgtacattg gtgcgccgtt ggcgaaagac agagaaaccc ttttcttcac
    32041 tagcgtacgg gcagtccctt caacaacgaa gcgggaagag ggaaataccc tgaagattgc
    32101 cacacaaagc gtcatcaaac ttttctggcg accaaaaggt ttagcgtatc ccttaggcga
    32161 ggctccggcg aaactgcgtt gcacttcgtc agctgacatg gttacggtca gtaacccaac
    32221 accttatttc attaccctga cagacctgaa aataggtgga aaagtagtta aaaatcaaat
    32281 gatttccccc tttgataaat accaattttc tctgccaaag ggggccaaaa atagcagcgt
    32341 aacgtatcga accatcaatg actacggggc ggaaacgccg caactcaact gtaaatcgta
    32401 agccgtcttc agttaagaga gcgagatgta tagaactcat cgacaacaca gcctgttaag
    32461 ctctggtgga gtgccatcgt ttattggtgg gctggtggtg tttgtgtcgg cagcgttcaa
    32521 tgcacaagct gaaacctggt tcgatcctgc ttttttcaaa gatgatccct caatggtggc
    32581 cgatttgtct cgtttcgaaa aaggacaaaa aataacgcca ggggtttatc gtgtcgatat
    32641 tgttctgaat cagacaattg tagatacgcg caacgtcaat tttgttgaga taacgccaga
    32701 gaaggggatt gccgcctgtt tgacgactga aagcctggat gcaatgggtg ttaatactga
    32761 tgcgtttccg gcttttaaac aactggacaa acaagcgtgt gtgccattgg cggagattat
    32821 tccggatgcc agcgtaacgt ttaatgtgaa taaactccgt ctggaaattt cagtaccgca
    32881 aatcgccatc aaaagtaatg cccgtggtta tgtcccccct gaacgttggg atgaagggat
    32941 caacgcgcta ttactgggat attcgtttag cggggctaac agtattcata gcagcgcaga
    33001 cagtgattct ggcgacagct attttctgaa tttaaacagt ggcgttaatt taggcccatg
    33061 gagattgcgc aacaattcaa catggagtcg cagtagtggc caaaccgcag aatggaagaa
    33121 tctcagcagc tatttgcagc gggcggttat tccactaaaa ggcgaactga ccgtaggtga
    33181 tgattatact gcaggcgatt ttttcgatag tgtcagcttt cgtggtgtgc agctggcgtc
    33241 agatgacaac atgctgccag acagcctgaa agggtttgcg cctgtggtgc gtggtatcgc
    33301 caaaagcaat gcccagataa cgattaagca aaatggttac accatttacc aaacttatgt
    33361 atcgcctggt gcttttgaaa ttagtgatct ctattccacg tcgtcgagcg gtgatttgtt
    33421 agttgaaatc aaagaagcgg acggcagcgt caatagctac agcgtaccgt tttccagcgt
    33481 gccattactc cagcgtcagg ggcgaatcaa atacgcggtg acactggcga aatacagaac
    33541 caatagtaat gaacagcagg agagcaaatt tgcccaggcc acgttgcagt ggggcggacc
    33601 gtggggaacg acatggtatg gtggtggaca atatgctgaa tattaccgtg ccgccatgtt
    33661 tggtctggga tttaaccttg gcgatttcgg agcaatttcg ttcgatgtga cccaggcgaa
    33721 gagtacgctg gcagaccaaa gcgaacataa aggtcagtca tatcgttttc tgtatgccaa
    33781 aacgctcaac caattgggca ctaattttca attgatgggc tatcgctatt cgacgtcggg
    33841 tttctacacc ctttccgaca ccatgtataa acatatggat ggctacgaat ttaatgacgg
    33901 tgatgatgaa gatacgccga tgtggtcgcg ttattacaat ttgttttaca ccaaacgtgg
    33961 caaactgcag gtcaatatct cccagcaatt aggcgagtac ggttcgtttt atttaagtgg
    34021 tagccagcaa acttactggc ataccgatca acaggatcgg ctattacagt ttggctacaa
    34081 cacgcaaatt aaagatctct cgctgggggt ttcctggaac tacagtaagt cccgtggtca
    34141 acctgatgct gatcaggtgt ttgcactaaa tttttccctg ccgctcaatc tgttgctccc
    34201 cagaagtaat gatagctata ccaggaaaaa aaattacgca tggatgacct ctaacaccag
    34261 tatcgataac gaagggcaca ttacacaaaa cctgggttta acggagacac tactcgatga
    34321 cggtaacctg agctacagcg tgcaacaggg atataacagc gaggggaaaa cggctaatgg
    34381 tagcgccagc atggactaca aaggggcgtt tgcagatgcc cgagtgggct acaactacag
    34441 cgataacggc agtcaacaac aactgaacta cgctctttca ggcagtttag ttgcccattc
    34501 acagggcatt accctggggc aatcgctggg ggaaactaac gtcctgattg cagcaccagg
    34561 cgcagagaat actcgtgtgg cgaacagcac cgggctgaaa actgactggc gcggatatac
    34621 cgttgtcccc tatgccactt cttatcggga aaatcgaatc gcacttgatg cggcatcgtt
    34681 aaaacgtaac gtggatcttg aaaatgcagt agtcaacgtg gttcccacca aaggggcgtt
    34741 ggttctggcg gagttcaatg cccatgcggg agcaagggta ttaatgaaaa catcaaagca
    34801 gggtataccg ctgcgttttg gcgcgatagc gacgctggac ggcatacaga ctaatagcgg
    34861 cattattgat gatgatggct cgctctatat gtccggtttg cccgctcagg gagcgataac
    34921 tgtacgctgg ggcgaagctc ccgatcaaat ttgtcatatc agttaccagc ttaccgaaca
    34981 acaaattaac tctgcgatta cgcgaatgga tgccatatgc agataatctt tggagaaaaa
    35041 tgcgtgttat tactacgact attttttgcc gccgtcttaa tgctatggtg cgctcaaact
    35101 gctgcttata gcgggcagtg tcataccact caggggaatc cgtatattgg cgtcaatttt
    35161 ggcgttaaaa ccctggagga agaagaaaat acggctggag tagttaaaga caaattttat
    35221 cagtggaacg aatcgaatga ttattatgtt tcctgtgatt gcgataaaga caatgtcaga
    35281 agtggccgat gggcattcgc cgcggattca ccgttagtct atttaggcga caactggtac
    35341 aaaattaatg actatcttgc cgccaaagtt ttattgcagg ttaaaagcag ttctcctact
    35401 gcggttcctt tcgaaaacgt gggcacaggg gcagatacac gatggcatat ttgcgatccc
    35461 ggcggtcaac gtttaggtgg ccagggggct agcggtaata gcggtagctt ttccctgaaa
    35521 atattgcagc cgttcgttgg ttcggtcgtc attcctccta tggcgctggc gcgattattt
    35581 gaatgctaca acatacccgc aggtgattcc tgcacgacta caggtacatc ggttttagtg
    35641 tattacctgt ctggtacgat caattcactt ggctcatgtt ccgtcaatgc cggagagact
    35701 atcgaggtcg atctgggcga cgtatttgcg gctaactttc gtgttgtagg gcataaaccc
    35761 cttggggcca gaacggcaga actcgcaatt ccagtcaggt gtaacacggg aaacgcgggg
    35821 ttagttaacg tcaacctgag tctgacggca accacagacc ccagctatcc ccaggcgatt
    35881 aagacgtcac gtcctggcgt gggcgtggtg gtgaccgata gccagaacaa cattatttcc
    35941 cctgctggtg gaacattacc gctctctatt cctgatgatg cagacagtat cgcgcgaatg
    36001 aatgtctatc cagtcagcac gacaggtgta ccaccagaaa ccgggcgatt tgaagccacg
    36061 gcaacggtga gaataaattt tgattaacgc gtgaacgtat gaagaaaaaa acgatatttc
    36121 agtgcgttat cttgttcttt agcattctta acatccatgt cgggatggct gggcctgaac
    36181 aagttagtat gcatatttat gggaatgtgg tcgatcaggg ctgtgatgtc gccaccaaaa
    36241 gtgcattaca aaatattcat attggtgatt ttaatatcag tgattttcag gccgcgaata
    36301 ccgtaagcac tgctgctgat ttgaatatcg atatcaccgg ttgtgccgct ggtattactg
    36361 gcgcggacgt cctttttagc ggcgaggctg acacccttgc gccgacactg ctcaaactaa
    36421 ctgacacagg cggaagcggt ggtatggcaa cggggattgc cgtgcaaatt cttgatgcgc
    36481 aaagtcagca agaaatcccg ctcaatcagg tccagcctct tacgccctta aaagccgggg
    36541 ataacacact caaatatcaa cttcgttata agtccacaaa ggcgggagca acgggcggta
    36601 atgcgacggc ggttctctat tttgatctgg tttaccagtg aaggagtgat gttgaaacgg
    36661 ataatctgga ttctgtgctt actgggatta acggggagta gtgagttatt tgctcatgat
    36721 ggcacggtca acattagcgg atcgtttcgt cgtaatacat gcgtgctggc acaggatagc
    36781 aagcaaatca atgtgcagtt aggcgatgtc tcgctgacac gtttttctca cgggaattat
    36841 ggcccggaga aatctttcat cattaatttg caggattgcg gaacagatgt cagcacggtt
    36901 gacgtcacct tttcaggaac ccccgacgga gtgcagagcg aaatgctgtc cattgaaagt
    36961 ggtatggatg cagccagcgg gctggcaatt gcgattctgg atgatgcgaa gatattgatt
    37021 ccgcttaacc aggcgagtaa agactacagc ctacacagcg gtaaggtgcc attaactttt
    37081 tatgcgcaat tacgacctgt caatagtgat gtgcagtcgg gtaaagtgaa tgccagcgcg
    37141 acatttgtac ttcattatga ctaatacgtg gaacagattg gcgctcttga tttttgccgt
    37201 tttatcgctg ctggtggcgg gtgaattgca ggcaggagtg gtggtcggcg gaacgcgatt
    37261 tatctttccg gcagacaaag aatcgatagc tattttactg actaatacca gtcaggaatc
    37321 ctggcttatt aacagtaaaa tcaatcgccc aacgcgttgg gccggggggg aagtgtcgac
    37381 agtgccagca ccattactgg ccgctccacc gcttattctc ctgaagcccg gtactacggg
    37441 cacgttgcgc ttgctgagaa cggaaagcga catcttgcca gtggatcgcg aaacgctatt
    37501 tgagttaagc attgccagcg tgccatccgg caaagttgaa aatcagagcg taaaagtggc
    37561 gatgcgctcg gtatttaaac tgttctggcg acccgaaggt ttgccgggcg acccgctgga
    37621 agcttatcaa caattacgct ggacacggaa ttcgcagggt gtgcaactca ctaacccaac
    37681 gccttactac attaacctga ttcaggtgag cgtaaatggt aaagcgttaa gtaatgcggg
    37741 agttgtgcca cctaaaagcc agcgtcagac aagctggtgt caggcaatcg caccgtgtca
    37801 tgtcgcctgg cgggcgataa atgattacgg cgggttgagt gcaaagaagg agcaaaatct
    37861 gccctgaaac aggttcggaa aacgtttgcg ttttttttgc cgcaggtcaa ttcccttttg
    37921 gtccgaactc gcacataata cgcccccggt ttgcacaccg ggaatccagg agagttcatg
    37981 tactacccct tcgttcgtaa agcccttttc cagctcgatc cagagcgcgc tcatgagttt
    38041 acttttcagc aattacgccg tattacagga acgccgtttg aagcactggt gcggcagaaa
    38101 gtgcctgcga aacctgttaa ctgcatgggc ctgacgttta aaaatccgct tggtctggca
    38161 gccggtcttg ataaagacgg ggagtgcatc gatgcccttg gtgcaatggg gtttggctcg
    38221 attgagatcg gcactgtaac gccgcgtcca caacctggaa atgacaagcc gcgtcttttc
    38281 cgcctggttg atgccgaagg tctgatcaat cgaatgggct ttaacaatct cggggtggat
    38341 aatcttattg agaatgtaaa aaaagcccat tatgacggcg tcctgggtat taacatcggc
    38401 aaaaataaag atacgccggt ggagcagggc aaagatgact acctgatttg tatggataaa
    38461 atttatcctt atgctggtta tatcgccatc aatatttcct cgccgaatac accgggatta
    38521 cgcactctgc aatatggtga agcgctggat gacttattaa tcgcgatcaa aaataaacaa
    38581 aacgatctgc aaaaaatcca tcaaaaatat gtgccgatcg cagtgaagat cgcgccggat
    38641 ctttctgaag aagaattgat ccaggttgct gacagcttag ttcgccataa tattgatggc
    38701 gttattgcaa ccaataccac actcgatcgt tctctggttc agggaatgaa aaattgcgat
    38761 caaaccggtg gcttaagtgg tcgtccgctt cagttaaaaa gcacagaaat tattcgccgc
    38821 ttgtcacagg aattaaacgg tcgcttaccg atcatcggtg ttggaggcat cgattcggtt
    38881 atcgctgcgc gtgaaaagat tgctgcgggt gcaacgctgg tgcaaattta ttcgggattt
    38941 attttcaaag gaccgccttt gattaaagaa atcgttagca atatctgatt aatttctcct
    39001 ttttaacgtt atgagacaac cggggcttta tttccagccc tgattgtttt atattcctta
    39061 cctgttgctt atttatacat tttagtcttt tcttatttag ggcaataaac gaagcggcaa
    39121 aggacaatgt tgcgcagaat gatttaggtg ggagaatccg catgcgaatt aaacctgacg
    39181 ataactggcg ctggtattat gatgaagagc acgatcgaat gatgctcgat ttagccaatg
    39241 gtatgttatt tcgctcgcgt tttgctcgta aaatgctcac accagatgcc tttaatccct
    39301 ccggtttttg tgttgatgat gcagcactct atttttcttt tgaagaaaaa tgccgtgatc
    39361 tgccgttatc caaagaacaa aaagccgaac tggtgctgaa tgcactggta gcaattcgtt
    39421 atctcaaacc gcaaatgccg aaaagctggc attttgtctc ccatggtgaa atgtgggtgc
    39481 ccatgcaggg ccctgcggcc tgcgtttggt tgagcgacac taacgagcaa gtgaatttac
    39541 tggttgttga atctggcgag aatgccgcgc tatgtctgct ggcgcaaccc tgcgttgtta
    39601 ttgcgggtcg cgcgatgcag ttgggcgacg ccattaaaat catgaacgac aggctgaaac
    39661 cgcaggttaa tgttgacagc ttcagccttg aacaggcagt ctaacgcgtc agcttaaggg
    39721 cagtcttcgg tacacagcta cagcaaagaa tggtgccatc atcgcccatt gctgattttt
    39781 tcagcggcgt gacttcgcct tctaaaagct gaacacggca acttccacaa atgcccgcgc
    39841 ggcaagaata agggatacga attccctgat tttctaattg ctccagcaac acttgttggt
    39901 tatttccacg aaatgcctgt ccctgccagt caatatctac attggcgtcc ggttgttgcg
    39961 tgatgttggc ggtatcatca gcggcagctg cgccgtaaat ttttgccgga gccgttgcca
    40021 gaatttccac ctcatcgcca acgcgaatca cgccgctatt acgggcaatt aaattctgac
    40081 caaaatcgac atcgccgtta tcctgggcag tgcggaaaga ttgcaatgtt tttaatggtt
    40141 cgcctgccgg atgtttttgc cctttttctg ggctgacggt ggtgaaaata cagcggctac
    40201 aaggtttaac cacatcaaac accacatcac caatgcgaat cactttccag cgatcttctt
    40261 cccaggctga cgcgccggaa accaccagat tggggcggaa ttgctccatt tttacactgg
    40321 ccggacaacg ttgttggaga tcacgtaacg aggcttcgtt agcaagaagg taaggatagc
    40381 catcagcaaa tgacaggggg acagtgttgt ggcgtttcac gcgccgggtc atttgtggcc
    40441 ccacccagcg taattgcact tcgcgggaga aaaatccact taaccatttg ttgatcgcgt
    40501 ctggcgcaat tcgcgcggta aaatgtgtgc cccaaacttc ggttggtgcg tcttgtgtgg
    40561 cgaaatcagc aaaacggaca tatgcgctac tgccatctgg tgcggtgaga tgcaagccat
    40621 catgcacagg tgaaggagta aaccgtacca tctggggaaa ctggcgagcg gtaataaacg
    40681 taccatcagg ttccgtaatc ataaagatgc gatcgaaggc cagaccactg tcatctgcca
    40741 gagcatgtgt aagaccaatg ccgcgcatcg atttaacagg atgaataaaa agccgggtta
    40801 atgtcgccac ggtgcggtcc tcaaatgaaa ataagccctc aactttatga catgacgcgc
    40861 ttattagcta taatgcgcaa caattttctt agcgcctgtc ccattaggct attttatttg
    40921 tcagtttggc cctgggttgt gctcgaaatc ctcacgtact tgtgtacgct ccggtttctc
    40981 cgcgctgtcc atgttcagac tgactgcaac aattacgcct actgcggtag gctcttagag
    41041 taaaagtgac gatatgaatt ctctgtttgc cagtacggcc cgtgggctgg aagagctgtt
    41101 aaaaactgaa ctggaaaacc tgggggccgt tgaatgccag gtggttcagg gtggggtcca
    41161 tttcaagggc gacacacggc ttgtttacca gagcctgatg tggagccgcc tggcctcgcg
    41221 tattatgttg ccgctgggcg agtgtaaggt ttacagcgat ttagacctct atctcggtgt
    41281 tcaggcgatc aactggacag agatgtttaa tcctggcgcg accttcgctg tccacttcag
    41341 tggtttgaat gacaccatcc gcaacagtca gtacggcgcg atgaaagtga aagacgcgat
    41401 cgtcgatgct ttcacgcgga aaaatctgcc gcgtccaaat gttgatcgcg atgcgccgga
    41461 tatccgcgtt aacgtctggc tgcataaaga aaccgccagt atcgctcttg atctgagtgg
    41521 tgatggttta catctgcgtg gctatcgcga tcgtgctggt attgcgccga tcaaagaaac
    41581 cctggcagcc gctattgtga tgcgatccgg ctggcagcca ggaacaccgc tgctcgatcc
    41641 gatgtgtggt tccggtacgt tgctgattga agcggcgatg ctggcgaccg atcgcgcacc
    41701 aggcttgcac cgtgggcgtt ggggctttag cggctgggcg cagcatgatg aagctatctg
    41761 gcaggaagtg aaagcggaag cgcaaactcg tgcccgtaaa ggcctggctg agtatagctc
    41821 tcatttttac ggttcggaca gcgacgcacg ggtgattcaa cgtgcgcgca ctaacgcccg
    41881 tcttgcgggg attggtgaac tgatcacctt tgaggtgaaa gatgtcgcgc aactgaccaa
    41941 tccgctgccg aaagggccgt acggcacagt gttgagcaac ccgccatacg gggaacgtct
    42001 ggacagcgaa ccggcgctga ttgcgctgca tagcctgctg ggtcggatca tgaaaaacca
    42061 gtttggtggc tggaatctct ctttgtttag tgcctcgccg gatctgctaa gctgcttgca
    42121 gctgcgtgca gacaaacagt acaaggcgaa aaacggcccg ctggactgcg tacagaaaaa
    42181 ttaccacgtt gccgaaagca ctccagacag caaaccggcg atggtagcgg aagactacgc
    42241 caaccgcctg cgtaagaacc tcaa
//
LOCUS       DAFATA010000026        41177 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFATA010000026 DAFATA010000000
VERSION     DAFATA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.026"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..563)
                     /locus_tag="LGJ20_001865"
     CDS             complement(<1..563)
                     /locus_tag="LGJ20_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK2385934.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFY"
     gene            complement(691..1191)
                     /gene="recX"
                     /locus_tag="LGJ20_001866"
     CDS             complement(691..1191)
                     /gene="recX"
                     /locus_tag="LGJ20_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK2385935.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(1434..2495)
                     /gene="recA"
                     /locus_tag="LGJ20_001867"
     CDS             complement(1434..2495)
                     /gene="recA"
                     /locus_tag="LGJ20_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK2385936.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(2575..3072)
                     /gene="pncC"
                     /locus_tag="LGJ20_001868"
     CDS             complement(2575..3072)
                     /gene="pncC"
                     /locus_tag="LGJ20_001868"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBK2385937.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(3217..4301)
                     /gene="mltB"
                     /locus_tag="LGJ20_001869"
                     /pseudo
     CDS             complement(3217..4301)
                     /gene="mltB"
                     /locus_tag="LGJ20_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
     gene            4557..5120
                     /gene="srlA"
                     /locus_tag="LGJ20_001870"
                     /gene_synonym="gutA"
     CDS             4557..5120
                     /gene="srlA"
                     /locus_tag="LGJ20_001870"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK2385938.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            5117..6076
                     /gene="srlE"
                     /locus_tag="LGJ20_001871"
                     /gene_synonym="gutE"
     CDS             5117..6076
                     /gene="srlE"
                     /locus_tag="LGJ20_001871"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK2385939.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            6087..6458
                     /gene="srlB"
                     /locus_tag="LGJ20_001872"
                     /gene_synonym="gutB"
     CDS             6087..6458
                     /gene="srlB"
                     /locus_tag="LGJ20_001872"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK2385940.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            6462..7241
                     /gene="srlD"
                     /locus_tag="LGJ20_001873"
     CDS             6462..7241
                     /gene="srlD"
                     /locus_tag="LGJ20_001873"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBK2385941.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            7346..7705
                     /gene="gutM"
                     /locus_tag="LGJ20_001874"
     CDS             7346..7705
                     /gene="gutM"
                     /locus_tag="LGJ20_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBK2385942.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            7772..8545
                     /gene="srlR"
                     /locus_tag="LGJ20_001875"
                     /gene_synonym="gutR"
     CDS             7772..8545
                     /gene="srlR"
                     /locus_tag="LGJ20_001875"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBK2385943.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            8538..9503
                     /gene="gutQ"
                     /locus_tag="LGJ20_001876"
     CDS             8538..9503
                     /gene="gutQ"
                     /locus_tag="LGJ20_001876"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBK2385944.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(9500..11014)
                     /gene="norR"
                     /locus_tag="LGJ20_001877"
     CDS             complement(9500..11014)
                     /gene="norR"
                     /locus_tag="LGJ20_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBK2385945.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            11201..12640
                     /gene="norV"
                     /locus_tag="LGJ20_001878"
     CDS             11201..12640
                     /gene="norV"
                     /locus_tag="LGJ20_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBK2385946.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            12637..13770
                     /gene="norW"
                     /locus_tag="LGJ20_001879"
     CDS             12637..13770
                     /gene="norW"
                     /locus_tag="LGJ20_001879"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBK2385947.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(13996..16248)
                     /gene="hypF"
                     /locus_tag="LGJ20_001880"
     CDS             complement(13996..16248)
                     /gene="hypF"
                     /locus_tag="LGJ20_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBK2385948.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSTAVATLRARKHRPAKPLAVILPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(16401..16928)
                     /gene="hydN"
                     /locus_tag="LGJ20_001881"
     CDS             complement(16401..16928)
                     /gene="hydN"
                     /locus_tag="LGJ20_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBK2385949.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(17077..18087)
                     /gene="ascG"
                     /locus_tag="LGJ20_001882"
     CDS             complement(17077..18087)
                     /gene="ascG"
                     /locus_tag="LGJ20_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBK2385950.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            18347..19804
                     /gene="ascF"
                     /locus_tag="LGJ20_001883"
     CDS             18347..19804
                     /gene="ascF"
                     /locus_tag="LGJ20_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBK2385951.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            19813..21237
                     /gene="ascB"
                     /locus_tag="LGJ20_001884"
     CDS             19813..21237
                     /gene="ascB"
                     /locus_tag="LGJ20_001884"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBK2385952.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(21396..21866)
                     /gene="hycI"
                     /locus_tag="LGJ20_001885"
     CDS             complement(21396..21866)
                     /gene="hycI"
                     /locus_tag="LGJ20_001885"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBK2385953.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(21859..22269)
                     /gene="hycH"
                     /locus_tag="LGJ20_001886"
     CDS             complement(21859..22269)
                     /gene="hycH"
                     /locus_tag="LGJ20_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBK2385954.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(22266..23033)
                     /gene="hycG"
                     /locus_tag="LGJ20_001887"
     CDS             complement(22266..23033)
                     /gene="hycG"
                     /locus_tag="LGJ20_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBK2385955.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIIPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(23033..23575)
                     /gene="hycF"
                     /locus_tag="LGJ20_001888"
     CDS             complement(23033..23575)
                     /gene="hycF"
                     /locus_tag="LGJ20_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBK2385956.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(23585..25294)
                     /gene="hycE"
                     /locus_tag="LGJ20_001889"
     CDS             complement(23585..25294)
                     /gene="hycE"
                     /locus_tag="LGJ20_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBK2385957.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(25312..26235)
                     /gene="hycD"
                     /locus_tag="LGJ20_001890"
     CDS             complement(25312..26235)
                     /gene="hycD"
                     /locus_tag="LGJ20_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBK2385958.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(26238..28064)
                     /gene="hycC"
                     /locus_tag="LGJ20_001891"
     CDS             complement(26238..28064)
                     /gene="hycC"
                     /locus_tag="LGJ20_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBK2385959.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(28061..28672)
                     /gene="hycB"
                     /locus_tag="LGJ20_001892"
     CDS             complement(28061..28672)
                     /gene="hycB"
                     /locus_tag="LGJ20_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBK2385960.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(28797..29258)
                     /gene="hycA"
                     /locus_tag="LGJ20_001893"
     CDS             complement(28797..29258)
                     /gene="hycA"
                     /locus_tag="LGJ20_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBK2385961.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            29470..29820
                     /gene="hypA"
                     /locus_tag="LGJ20_001894"
     CDS             29470..29820
                     /gene="hypA"
                     /locus_tag="LGJ20_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBK2385962.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            29824..30696
                     /gene="hypB"
                     /locus_tag="LGJ20_001895"
     CDS             29824..30696
                     /gene="hypB"
                     /locus_tag="LGJ20_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBK2385963.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            30687..30959
                     /gene="hypC"
                     /locus_tag="LGJ20_001896"
     CDS             30687..30959
                     /gene="hypC"
                     /locus_tag="LGJ20_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBK2385964.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDKNGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            30959..32080
                     /gene="hypD"
                     /locus_tag="LGJ20_001897"
     CDS             30959..32080
                     /gene="hypD"
                     /locus_tag="LGJ20_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBK2385965.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            32077..33087
                     /gene="hypE"
                     /locus_tag="LGJ20_001898"
     CDS             32077..33087
                     /gene="hypE"
                     /locus_tag="LGJ20_001898"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBK2385966.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            33161..35239
                     /gene="flhA"
                     /locus_tag="LGJ20_001899"
     CDS             33161..35239
                     /gene="flhA"
                     /locus_tag="LGJ20_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBK2385967.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(35276..35629)
                     /locus_tag="LGJ20_001900"
     CDS             complement(35276..35629)
                     /locus_tag="LGJ20_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBK2385968.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(35661..35840)
                     /locus_tag="LGJ20_001901"
     CDS             complement(35661..35840)
                     /locus_tag="LGJ20_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385969.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            35916..38477
                     /gene="mutS"
                     /locus_tag="LGJ20_001902"
     CDS             35916..38477
                     /gene="mutS"
                     /locus_tag="LGJ20_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK2385970.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            38583..39239
                     /gene="pphB"
                     /locus_tag="LGJ20_001903"
     CDS             38583..39239
                     /gene="pphB"
                     /locus_tag="LGJ20_001903"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBK2385971.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNNNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(39290..40057)
                     /locus_tag="LGJ20_001904"
     CDS             complement(39290..40057)
                     /locus_tag="LGJ20_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2385972.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            40253..41161
                     /locus_tag="LGJ20_001905"
     CDS             40253..41161
                     /locus_tag="LGJ20_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK2385973.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
BASE COUNT         9682 a        11290 c        10867 g         9338 t
ORIGIN      
        1 tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt cacccatctg ccagaagttg
       61 tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc
      121 cctacttctt tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac
      181 agacgctctt tcggcagggc aaaccatttt tcgctggtca gcagttccca tgcaaactga
      241 atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta
      301 tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg
      361 cagcgttggg aagtggtagc gcgggaataa ttacgcttgt cgagcccaag gaacacatcc
      421 ttgaactggt tcatcccggc gttggtaaac aacaaagttg ggtcgttatg gggtaccagg
      481 gagctgctgg caactacctg atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg
      541 atctcagcgg tgctcttgct cataattatc ctgaaatcaa gctaacgaaa tatcgccacc
      601 agctccagcg tgtcttaacc gccgggctgg taactgaaaa gtgggaataa gataagtttt
      661 cttgactggg aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg
      721 gatatcttcc atcagatagc cacgatagag caaaaaacgc tggatcttaa ctttttctga
      781 aaagacagtt ggcaaaggtt caccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca
      841 ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg
      901 attcagttcc tggcgaatac gcgcaggtcc ataacctttg cggctacggc tggcgataaa
      961 gcgcgcaaca aatcggctgt catcgagata gccatgttca tggcaccagg caataacgcg
     1021 ctcgtaatct tctgccgtag catcaatctc ttctgggcca tttttgccca taatcggtgc
     1081 cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc
     1141 acgatccaac aggcgagcat atgccgggcg acgggacgtt gattctgtca tggcatatcc
     1201 ttacaactta aaaaagcaaa agggccgcag atgcgaccca ggttttgctg ataaacagat
     1261 ttaatagtca atgagtgtgc agaatgttcc cgcaggccca attcgttaga ggatcgaggt
     1321 gagagtaatt tttcacgcca gtgctggcct gttcccctca ccctaaccct ctccccaaag
     1381 gagcgagggg accgtccgtg cacacattat ctattatcat caataacgaa cgattaaaaa
     1441 tcttcgttag tttctgctac gccttcgctg tcatctacag agaaatccgg cgttgagttc
     1501 gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt ttccgggtta
     1561 tctttcagcc aggcagttgc attcgcttta ccctgaccga tcttctcacc tttgtagctg
     1621 taccacgcgc ctgctttctc gatcagcttc tcttttacgc ccaggtcaac cagttcgccg
     1681 tagaagttga taccttcgcc gtagaggatc tgaaattcag cctgtttaaa cggtgcagcg
     1741 attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc gccctctttc
     1801 accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag cgcgttacca
     1861 ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat ctggttgatg
     1921 aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat cgcctggctc
     1981 atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat ttccgctttc
     2041 ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg
     2101 gcgtcacaga tttccagtgc ctgctcgccg gtgtccggct gggagcacag caggttatca
     2161 atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc atcgataaac
     2221 gcacaggttt taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta
     2281 ccggaagatt ccggtccgta gatttcgacg atacggccca tcggcagacc acctgcccca
     2341 agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat ggaacggtct
     2401 tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc cagtgctgcc
     2461 gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat gccgggtaat
     2521 accggatagt caatatgttc tgttgaagca attatactgt atgctcatac agtatcaagt
     2581 gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct gacgacgcac
     2641 cgcatcacgg tcaccgctga agcattcccg ccgggtaatg ccttcaccgc gggcagtggc
     2701 aaaagcaaac cagacggtgc cgacaggctt ctcttcactg ccgccatccg gcccggcgat
     2761 accactaata gacacggcat aatcagcacg agccgctttc agtgcaccta tcgccatttc
     2821 caccacgacg ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc gtacgccgat
     2881 catctgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc aggcggagct
     2941 accggcaata tcggtaatca ctttcgctac ccaaccaccg gtacaagact cggcagttgt
     3001 tacggttgcg ccacgggctt tcagcgcctg cccaacctgt tcacttaact gcatcagttc
     3061 actgtcagtc atcacaacct ctgtcagtca aaaatttatg ccggacaaga tagcactttc
     3121 gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa aagctgatta
     3181 gccagaggga agctcacgcc cccctcttgc aaatagttac tgcacgcgcg ccagcgccac
     3241 ggcttgtcct aactgccaga ccgccattgc gtaatgggtg ctgtggttat aacgggtaat
     3301 ggtgtagaag ttcggcagac cgtaccagta ctggtaaccg gtaccgacat ccagacgcag
     3361 caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg ccgtggcaag
     3421 ctgcgaaatg ctgtacctgg ttttgaagcc atttggcaag cctggagcct gaccgtttgc
     3481 cattaccgcg acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt tcgccacgct
     3541 accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga aatctaccgc
     3601 atattgtttg taagacgacg gcataaactg tccgtagccc atcgccccgg caaaggaacc
     3661 tttcagattg agcggatcgt cctgctcgtc gcgcgcatca acaggaaggt ttccagctcg
     3721 ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg
     3781 atgcgagttt tccccatcac gcgcccccag cgggtttcaa cgccgataat cccgacgata
     3841 atttccggcg gtacgccata cacctgccac gcgcgattca acgcatcttc atactgattc
     3901 cagaaaacca caccgttctg cacgttgtcc ggcgtaataa attttttgcg ataacggagc
     3961 catgcgccgt tcggacctga tggcggtttc accgatgtgg ttggtgcctg gttatccatc
     4021 agccgcagta ccgaatccag acgcttcgcc tgggagagaa tttcctgcaa ctgctgacga
     4081 tcgaaaccgt gtttgttcac cattttgtcg atgaactgct gggcattcgg gttattagcg
     4141 aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc gccagacggc
     4201 gttccggtgg tcgtttcagt ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac
     4261 acaaaaaggg gaagcaatgt tacataacga cgcttgaaca tgaggggtcc atttaacaga
     4321 ttcaaccagg ggcaagtatg gtaaagcatc acgccccaca caaggaagcg gtagtcactg
     4381 cccgatacgg actttacata actcaactca ttcccctcgc tatcctttta ctcaaacttt
     4441 caaattaaaa tatttatctt tcattttgag atcaaaataa cacttttaaa tctttcaatc
     4501 tgattagatt aggttgccgt ttggtaataa aacaataaat cctgaaggag agaacaatga
     4561 tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag ggcggagagg
     4621 tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgctg gttatcatga
     4681 acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa cgttgcgccg
     4741 gtaaccctgt ttcccgttac ctactgttac cgtgcattgg cacgtttgtc ttttgcaatc
     4801 cgatgacctt aagcctgggt cgctttatgc cggaaaagta caaacccagc tactacgcgg
     4861 cggcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac cctggcgaac
     4921 tgtttgttta tcttggcatt gccagcggtc tgacaacgct gaacctgcca cttggcccac
     4981 tggcggtgag ttatctgctg gttggtctgg tcaccaattt cttccgcggc tgggtgaccg
     5041 atctgaccac cgccattttt gagaaaaaga tgggcattca acttgaacaa aaagttcacc
     5101 tggcaggagc aacatcatga cgcatattcg gatcgaaaaa ggaacgggtg gctggggcgg
     5161 cccgcttgag ctgaaagcca cgccgggaaa aaaaatcgtc tatatcaccg ccggtacccg
     5221 gcctgcgatt gttgacaaac tggcacagct tactggctgg caggctattg acggatttaa
     5281 agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta atcgactgtg gcggcacatt
     5341 acgctgcggc atctatccga aacgacgtat tcccaccatt aatatccact cgacgggcaa
     5401 gtccggtccg ctggcgcagt acattgtgga agatatttat gtctctggcg taaaagaaga
     5461 aaacatcact gtagtgggtg atgcgacacc acaaccctct tccgtgggcc gtgactatga
     5521 caccagtaag aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg gaatgggcat
     5581 ggggtccgcc gttgcggtgc tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa
     5641 aaccattctg ccgtttatgg cattcgtctc ggcgctcatt ggcatcatta tggcttctgg
     5701 ccttggtgac tggattgccc acggtcttgc tccgctggcg agccatccac tgggtctggt
     5761 catgctggcg ctcatctgct ccttcccact gctttcacct ttcctcggcc caggcgcagt
     5821 tatcgcacag gttatcggcg tattgattgg cgtgcagatt ggtctcggca atattccgcc
     5881 gcatctggct ttaccggcac tgtttgccat caacgcgcag gcggcctgcg acttcatccc
     5941 ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt
     6001 actggtgagc cgctttttaa ccggcgcgcc gactgtactg atcgcctggt ttgtctccgg
     6061 ttttatctat caatagaggc tgaaacatga ccgttattta tcagaccacc atcacccgta
     6121 tcggcgcgag tgccactgac gccctcagcg accagatgct catcaccttt cgtgaaggcg
     6181 cgccagcgga cctcgaagag tattgcttca ttcattgcca cggcgagttg aaaggtgcac
     6241 tccatcccgg tttgcaattt tcactcgggc agcatcgcta tccggtgacc gctgttggca
     6301 gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa
     6361 acgaagcgga atttccgggc actgtccatg tggcaggccc tgtccccgac gatatcgcgc
     6421 cgggatcggt tttgaagttt gaatctgtta aggagtaaaa aatgaatcag gttgccgttg
     6481 tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt
     6541 atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc aaatgtggca caagaaatta
     6601 acgccgaata tggtgaaagt atggcgtacg gttttggtgc tgacgccact agcgagcaaa
     6661 gcgttctggc gctctctcgt ggggtagatg aaatctttgg tcgcgtggat ttgctggtct
     6721 acagcgccgg aatagcaaaa gcagccttta tcagcgactt ccagctcggc gattttgacc
     6781 gttcgctaca ggtgaatctg gtgggttatt tcctgtgtgc gcgtgaattt tcgcgtttga
     6841 tgatccgcga cgggattcag gggcgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg
     6901 gcagcaaaca caactctggc tacagcgcag cgaaatttgg tggcgtcggg ctgactcaat
     6961 cactggcgct ggatctggcg gagtacggca ttacggtgca ttcactgatg ctcggtaacc
     7021 tgctgaaatc gccgatgttc cagtcactgt tgccacaata cgcgaccaag ctgggtatca
     7081 aaccggatca agtcgagcag tattacatcg acaaagtacc gctcaaacgc ggctgcgatt
     7141 atcaagatgt gctgaatatg ctgctgttct acgccagtcc taaggcgtcg tactgcaccg
     7201 gacagtcgat caatgtcacc ggcggtcagg tgatgttctg atcaacagcg gagatccatt
     7261 aaggatctcc gtgagactat agaatgcctg atgcgctacg ctcatcaggc atacaggact
     7321 tccgccacta cattaaggaa aagttatggt atccgcactc atcaccgtcg ccgttatcgc
     7381 ctggtgtgcg caactggcct taggcggctg gcaaatttct cgttttaacc gtgccttcga
     7441 cacactatgc cagcaagggc gggttggcgt gggccgttcc agcgggcgct ttaaaccgcg
     7501 ggtcgtggtc gccatcgcgc tggacgatca gcagcgcatc gtcgacacct tgtttatgaa
     7561 aggactgacc gtcttcgccc gaccgcaaaa aattcccgca attaccggta tgcatgcggg
     7621 tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg ctctatcatt
     7681 ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg cgaagttatc
     7741 attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc aggccgccat
     7801 tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc aatactttga
     7861 caccacaggc acaaccattc gcaaagatct ggtcattctg gaacatgccg gaaccgtcat
     7921 tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccta tcgatcataa
     7981 aacactcatc aacacccaca agaaagagct gattgcagaa gctgccgtta gttttatcca
     8041 tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg ttcccctgct
     8101 ctcgcgcttt aataacatca cggtgatgac caacagcctg catatcgtca atgcgctatc
     8161 cgaactggat aacgaacaaa ctatcctgat gccaggcgga acgtttcgta aaaaatcggc
     8221 ctcatttcac ggacagctgg cagagaatgc cttcgaacat ttcagtttcg ataaattgtt
     8281 tatgggcacc gacggcatcg atctcaatgc gggcgtaacc accttcaacg aggtttatac
     8341 cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa
     8401 gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata agctgattac
     8461 cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga tcgatgtgat
     8521 cataaccgga gagagcaatg agtgaagcac tactgaacgc gggacgtcag acgttaatgc
     8581 tggagttgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt gttcgcgccg
     8641 ccaatatcat cctgcactgc gaaggcaaag tggtggtttc gggaattggc aaatcgggcc
     8701 acattggtaa gaaaatcgcc gcaacacttg ccagtaccgg cacaccggct ttttttgtcc
     8761 atccggcaga agcactgcac ggcgatctgg ggatgattga aagccgcgat gtgatgctgt
     8821 ttatctctta ctccggtggc gcgaaggaac tggatctgat tattccgcgt ctggaagata
     8881 aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc ctggcggcaa
     8941 aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt gcgccgacct
     9001 ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc atgcaggcgc
     9061 gcggatttaa tgaagaagat tttgcccgct cccacccagc cggggcactg ggcgctcgct
     9121 tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccacag gtggcgttaa
     9181 ccgccagcgt gatggatgcg atgctggaac tcagccgcac cggtctgggg ctggtggcgg
     9241 tatgtgacgc tcaacaacag gtacaaggcg tctttaccga cggcgattta cgtcgctggc
     9301 tggttggcgg cggcgcactc accacgccag ttaatgaagc gatgacgaca ggcggcacca
     9361 cattacaggc acaaagtcgc gccatcgacg ccaaagagat cctgatgaag cgcaaaatca
     9421 ctgccgcacc ggtggtggat gaaaacggca aactcaccgg cgcaattaac ctgcaggatt
     9481 tctatcaggc cgggattatt taatccttca atcccagacg tttcgccagc cgatgcaggt
     9541 tggcgacgtc ggtttccagc atccgcgcgc aggcagccca gttgtgatga ttttgtgcca
     9601 gtgcctggcg aatggtttca cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa
     9661 caacgggcac cgccgccgct tctggcggcg gcaacgtcac ctcaggaaaa gcaaaatgtt
     9721 gtgcctcaag aatcacttca tcgccgctgc gagtggctct cgccagcact accgcccgat
     9781 gaatagcatg ttccagttcg cgcacgttcc ccggaaaacg atagtgttgc agtaaatttc
     9841 gcgctccggc acttaatacc acgcgggaga gccccagccg caaacgacac tgctcgcaga
     9901 aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc accgaaagtg
     9961 gaaacacgct caggcgatga aacaaatcgg cgcggaatcg ccctgccagc acctcttcgc
    10021 gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaacta cggtcatcgc
    10081 caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc aatgccaacg
    10141 acaactcgcc gatctcatcg agaaacagag tgccgttatc cgccatttca aacttcccgc
    10201 tgcggttact gatagcgcca gtaaacgctc ctttcacatg cccgaacaac tcactttccg
    10261 ccacactttc cggcagtgcg gcacagttga gatagaccag cggattcacc gcccgtggcg
    10321 aggcttcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca ccgctgatca
    10381 ggacgttgag atcggacgcc gccacaatct caatctcttt tttcagttgc gtcatgccag
    10441 gggacaagcc aatcatctgc gtctgtttca ccgcttcaaa cggcgtggca tcgcctggca
    10501 tcatattctg gctttccagt tgttcaatca gcaacgcatt gcttaacgct cccgccgcca
    10561 gcgcagcaat cagccgtagc tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc
    10621 cgtcgagcgt cagtgcgccg atcaggtttt gcccggcaaa caatggcaga ccaacgcagg
    10681 cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc atagggatcg ggcaattcgc
    10741 tgtctgcagg aaagcgcacc acgtccccgg cgcgggcaat cgcttcaagc cgtggatgcc
    10801 cttccagcgc aaagcgtcta ccgagtacat cctttgccag accgtcgatg gcaagcggaa
    10861 taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc gcactccagc acctgacgta
    10921 gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc aattcgatgg
    10981 cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca atttgactat
    11041 agatattgtc atatcgacca tttgattgat agtcattttg actacccatt aatgagcata
    11101 cttttattta tatagtaaaa acaatcagat aaaaaactgg cacgcaatct gcaattagca
    11161 agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg tggtgaaaaa
    11221 taacattcat tgggttggtc aacgtgactg ggaagtgcgt gattttcacg gcacggaata
    11281 taaaacgctg cgcggcagca gttacaacag ctacctcatc cgcgaagaaa aaaacgtgct
    11341 gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc gtaatgaaat
    11401 cgatctggcg gatatcgatt acatcgtgat taaccatgca gaagaggacc acgctggggc
    11461 gctgaccgaa ctgatggcgc aaattcccga tacgccgatc tactgtactg ccaacgctat
    11521 cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg tgaaaactgg
    11581 cgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacac caatgctgca
    11641 ctggccggac agcatgatga cttacctgac aggcgacgcg gtgctgttca gtaacgatgc
    11701 tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc agacggagct
    11761 tttcgagcag tgccagcgtt actacgccaa tatcctgacg ccgttcagcc gcctggtaac
    11821 gccgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag ccacttccca
    11881 cggcgtggta tggcgcgata acccgacgca aattgtcgag ctgtacctga aatgggcggc
    11941 tgattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata acacccgcat
    12001 gatggctgac gctatcgccc aggggattgc ggaaaccgac ccacgcgtgg cggtgaaaat
    12061 tttcaacgtc gcccgaagcg ataaaaacga aatcctgact aatgtcttcc gctcaaaagg
    12121 cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg ccgggctggt
    12181 ggaagagatg actggtttac gcttccgtaa caaacgcgcc agtgctttcg gctctcacgg
    12241 ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg gtttcgaaat
    12301 gtcgcttagc ctgaaagcca aatggcgacc agaccaggac gctctggagt tatgtcgtga
    12361 acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa
    12421 tacggtggtt aaagaagaaa cctctgccac cacgacggct gacctcggcc cacggatgca
    12481 gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc
    12541 gccaggaacg ccgtggagtg aagtcccgga taacttcctc tgcccggaat gctccctcgg
    12601 caaagacgtc tttgacgaac tggcatcgga ggcaaaatga gtaacggcat tgtgatcatc
    12661 ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga cgccactatt
    12721 ccattaaccc tgattgccgc cgacagcatg gatgagtaca acaaacctga cctcagccat
    12781 gttatcagtc aggggcaacg tgccgatgac cttacccgcc agacggcggg tgaatttgcc
    12841 gagcagttta atctgcacct gtttccacaa acctgggtga cggatatcga tgccgaagcc
    12901 cgtgtggtga aaagccagaa taatcagtgg caatacgaca agctagtact ggcaaccggt
    12961 gccagtgcct ttgtcccgcc tgtgcctggg cgtgagttaa tgctgacgtt aaatagtcag
    13021 caagagtatc gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt gattgttggc
    13081 ggtggtttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa agcggtcacg
    13141 ctaatcgaca acgctgccag tattctggcg tcattaatgc caccggaagt aagcagccgc
    13201 ttgcagcatc ggttgacgga gatgggcgtt catctgttgt tgaaatctca gttgcagggg
    13261 ctggaaaaaa cagattctgg cattctggca acgctggacc gccagcgcag tatcgaagtg
    13321 gatgcggtaa ttgccgccac cggactgcgc ccggaaaccg ccctggcacg acgcgccggg
    13381 ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa tgccgatatt
    13441 tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct ccagccgatt
    13501 caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc actgaaacta
    13561 ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc aggcgaaacc
    13621 cagcgtcagg atttacgctg gcaaattaat accgaacgcc agggaatggt ggcgcgcggc
    13681 gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg aggatcggat gaaagaggca
    13741 tttggattgt tgaaaacatt gccgatgtag gtgggctact gtgcctaaaa tgtcggatgc
    13801 gacgctggcg cgtcttatcc aacctacggg gagcgcatgt gttggtcaca tactgcgttc
    13861 atgtcgcatc cacctactgt gcccaaaatg tcggatgcga cgctggcgcg tcttatccga
    13921 cctacgggga cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg caatggtgtc
    13981 caaatgcaac acgttttatc cgttctggac ttcacccgct aaccaacgcg ccgcagcaat
    14041 aaccccctgc cccagagaca aaccgccatc acccgccggt aaactctgtg gaaagagcaa
    14101 tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat gaataacccc
    14161 gccgctaaat accagcgtag tgataccacg catcgtggcc tgctcacgca tcaacgcggc
    14221 aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca
    14281 gttcagccac tgctgccaga aagtggcgag atccagttga ttgtccacca gcggcattgt
    14341 caccggatgc gtcactccgt ggcacgaagc tgcgagtgcc tccagagcac aagccgcttc
    14401 accttcataa cttaacgtgg ctggcgcaca gcccagtgcc gccgccacgg catcgaacaa
    14461 ccgcccacac gatgacgcca gcggcgcgtt aattccacgc tcaatggccc gcgccagcac
    14521 gctccagttt tgctgttgca cacttgctgt ttcagaataa ttctgccact ccggcacaaa
    14581 gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc gccgccaaat cgccacccgg
    14641 aagcgccact gcaggcaagc cgcccaggtg ctcacattcg cgatagttca cccgcagaca
    14701 ctcgccgccc cacaaagcgc cgttctcccc cataccgata ccatcgagcg tcaaagcaat
    14761 gacatcaccg ccatccagcg gccactgatg ctctgccaga cacgccgctg cgtgggcatg
    14821 atgatgcagc accgtttgcg tcggcagatt catttcgcgc gcccactggc tggagacata
    14881 gcccggatgt gcgtcatgca caacgtattg cggggtgaaa tcgtagatgt tttgcatcag
    14941 gcgtaacgct tcgcgccact gcatctggat gccatcgtca cttaaatcgc ccagatgctg
    15001 actcaacact gcttgttcac cgcgcaccag gcagaaggtg tttttcagat ctgcgccgag
    15061 acacagcaca ggcggcacat ttttaaagcc cggaggcaaa gccagtgcgt ccggtacata
    15121 cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc accgaatcat ccatccgctg
    15181 cacgatgtcg cggttatgta tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc
    15241 ctgttcgttg ctgatagccg gtggtttacc gctcaggttg ccggaggtca tcaccagcgg
    15301 gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca tcacgccgac
    15361 ttcgttaagg ccaggggcga tatcatcaca aagctcagga acgtattttt tatccaccag
    15421 cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta aaccttctgc
    15481 cactggcaag atgaccgcca gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc
    15541 caccgcggta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc ctttgatggc
    15601 gacaatgttg cccattttta actgtgcgat agccgcctgt aatgccgcct cttgttccgc
    15661 atgttcacca tgacttaccc attcaagatg tgggccacac tccgggcagg ccaccggctg
    15721 ggcgtggaag cgacgatcga gcgggtcgcg gtactcttta tcacaggctg gacacagcgg
    15781 aaactccgcc attacggtaa acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg
    15841 cgggccgcag tgggtacagt tgataaacgg ataacgataa cgccgttcgc ctggggtatt
    15901 catttcggca aggcaagcag ggcaagtagc ggcatccggg acaatttgcg tattcatggc
    15961 acctcccgcg ctctggcgga tagtgaactc ggtgggcagt tgtgaccaga taaacggctc
    16021 acgctcgacg ctatcaatac gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa
    16081 cgtttccggg tcttccagca gccggacttc tacgccatcg ccgtcattac agacatcgcc
    16141 gtgaagattt aattgctgtg ccagctgcca gacaaacgga cgaaaaccga cgccctgcac
    16201 tttgccacga atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc
    16261 gccatcatga attgcgtaac ccgtccagcc ggacacgaca gcgtcgcatc cggcagtcac
    16321 aggtcggcga taccgcccgc ttcgtattct acgaatattt ccgggaattc ctttgatgcc
    16381 agaacagttc tgtaagattt ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc
    16441 agttgttcaa gtttattacg atcgacacaa attagcgcat gggtcgggca agccgccata
    16501 cacgccgggc cgtcttcacg atggttgcac aggtcgcatt tattggcttc agctttgtca
    16561 gcccgtacat tcagccccgc gccgctgttg cggatcaccg gacgtaccac cacttccatc
    16621 gcgccatacg ggcaagccac cacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca
    16681 tgaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca
    16741 tcttcacact gacggcagac tgtcgccgtg gaaatgttca cacctttaat gacatggatt
    16801 cgcggtaaaa aggtttccgg ggtcagcgat gcacagtcct gattttcctg atgagaaacc
    16861 acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa
    16921 cggttcatca aattctccag caatgacagt taatgcgccg atacattcac aaatcatgcc
    16981 agtttttaat ttgctgttat ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc
    17041 atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaaggg caatgagtga
    17101 gtcgcggcgg atcagttttc cgctgaaggt tttcggcggt gagaaatccc cgccatcgag
    17161 cataaaaatc agccgtccaa taatttcctg aatcatctct gttaccggaa tttttacgct
    17221 ggagagcgcc ggaacggtgt agggggcaat tgcgatatca tcgaatccga taactgacac
    17281 ctgctctggc accgctacgc cgcgctcgtg caacgctttc atcgcaccta tcgccatatc
    17341 gtcgttactg gcaactaacg cgctaaattt agccccacgt tcgagcaacg tttctacccc
    17401 ttcggccccg ctggcgggcg tccatttacc gttagcgata agtttttcat tgagcgcaat
    17461 accatgctgc gccagcgcgt ctttataccc agcaagacgt tcaatgctgg tgggggaatc
    17521 catcgagccg gtaaggaaag caatctcctg atgtccggcg tttatcaact ctgccacggc
    17581 attaaagctg gtctgtttat gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg
    17641 gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcaa tctcatccac
    17701 gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca gatactgaat
    17761 cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact gccgcccttt
    17821 ctcttccgcc attcgcgcgg catgaaacag taattcacta aaataaatgc cgtgataaag
    17881 cgtgttggtc actaccagcc ccagcgtctg agtactcttc gccgacagat tgcgggccag
    17941 caaatttggg cggtaaccgc tctcttctac cgcctgaaac acgcgatctt tagtctcctg
    18001 gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg
    18061 cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt
    18121 gtactgcacc gtttgccgat tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg
    18181 gtttcacaaa tataaaaaat gaacaattaa ctctcttgct tatttagtga caactattca
    18241 tgattttgtg aaaccggttt cctaattccg tttcagcatc ggcatttttc cgttacgtcg
    18301 actgataaca actacatcta ccctactgat aacaggataa aatccgatgg ccaaaaatta
    18361 tgcggcgctg gcacgctcgg tgatagcggc actgggcggc gttgataaca tctcggcggt
    18421 cacgcactgt atgacgcgtt tgcgctttgt tatcaaagat gaccagctta tcgacagccc
    18481 gacgttaaaa accatccccg gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt
    18541 gattatcggc aataccgttt cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga
    18601 tatgcagccc gcacagcccg tgggtaaacc caaactcacg ctgcgtcgca ttggtgcagg
    18661 gatcctcgat gcgctgatcg gcaccatgtc accgctgatc ccggcgatta tcggcggatc
    18721 gatggtcaaa ctgctggcga tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc
    18781 gaccttaacc attctgaatg tgattggtga cggtgctttc ttcttcctgc cgctgatggt
    18841 cgcggcatct gccgccatca aatttaaaac caatatgtcg ctggcgattg ccatcgcggg
    18901 tgtgctggta catccgagtt ttattgaact gatggcgaaa gcggcccagg gtgaacatgt
    18961 cgaatttgcc ctgattccgg tcaccgcggt gaaatacacc tacacagtga tcccggcgct
    19021 ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta caccggcggt
    19081 gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc tggcaatcct
    19141 gctgattggc ccgattggta tctggatcgg tagcgccatt tcggcgctgg tttacaccat
    19201 tcatggttat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc ctctgctggt
    19261 aatgaccggg atgcaccgcg tctttacgcc aaccatcatt cagaccattg ccgaaaccgg
    19321 caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc
    19381 actggcggtg gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc
    19441 ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg cgatccgcct
    19501 gaaacgtccg cttatcgcca gtcttatcag cggctttatt tgcggcgcgg ttgccggtat
    19561 ggcggggctt gccagccact caatggcagc gccggggcta tttaccagcg tgcagttctt
    19621 cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtcat
    19681 gctgtcgttt atcctcacac tgttgctcgg cttcgaggat attcctgttg aggaagcgac
    19741 tgccgaggcg cgaaagcatc agagcgtaca accgaccgtc gccaaagaag taagtcttaa
    19801 ttgaggatga aaatgtcagt atttccagaa ggttttttat ggggcggcgc gcttgccgcc
    19861 aaccagtctg aaggtgcgtt ccgtgaaggt ggcaaagggc tgaccactgt cgatatgatc
    19921 ccacacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc
    19981 gatgacgagt tttatcccag ccatgaggcg acggattttt atcatcgtta taaagaagat
    20041 atcgccctga tggcagagat gggattcaag gttttccgta cctcaattgc ctggagccgt
    20101 ctctttccgc agggcgatga aatcacgccc aatcagcagg gcattgcttt ttatcgttct
    20161 gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat
    20221 gtgccgatgc atctggtcac cgaatatggc tcctggcgta accgcaagct ggtggagttt
    20281 ttcagccgct acgcccggac ctgctttgaa gcatttgatg gtctggtgaa atactggctt
    20341 accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg tctggtgttt
    20401 gaagaaggtg aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc
    20461 agtgcgctag ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt gggctgtatg
    20521 ctggcgggcg gtaacttcta cccttacagt tgcaagccgg aagatgtctg ggcggcgctg
    20581 gagaaagacc gggaaaacct gttttttatc gatgtgcagg cgcggggcgc gtatccggct
    20641 tactctgccc gcgtattccg cgaaaaaggg gtaaccatcg acaaagcacc gggcgatgat
    20701 gaaatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc gcgctgcgcc
    20761 tcggcggaga tgaacgccaa caatagcagt gcggcgaacg tagtgaaatc gctgcgtaac
    20821 ccgtatctgc aagtgagcga ctggggctgg ggtattgatc cactcggtct gcgtatcacc
    20881 atgaacatga tgtacgaccg ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc
    20941 gcaaaagatg aacttgctgc caatggcgag attaacgatg actatcgcat cagctattta
    21001 cgcgaacata tccgcgcaat gggcgaagcg attgcagatg gcattccgct gatgggctac
    21061 accacatggg gctgtattga tttagtttcc gcctctacgg gtgaaatgag caaacgctac
    21121 ggctttgtct ttgttgaccg tgacgacgca ggcaacggta cgctgacgcg cacgcgtaag
    21181 aaatcattct ggtggtataa aaaagtgatt gccagtaatg gggaagattt agagtaggta
    21241 acagtgccgg atgcggcgtg aacgccttat ccggcctaca tgtcgcacga accatgaagg
    21301 cccgataagc gcagcgcatc gggcaattta gcatttggca tcagagctgg ttcaccccat
    21361 caagaacatc cctgtcctga ttccttaatg aaaaactact cttcttccac cgctaactgc
    21421 gcgaagccgc cattcccttc ccagccttcc agtcgttgat aaacggtttc taccgcatct
    21481 ttaatcggct gagtcatcgg gtagtaaaag ccgacgatat ccggctggat gccgaggaaa
    21541 atcacttcgc caatgtcttc tttcaactgg tcgataaggt agttcaacgg catgttatgg
    21601 gtagtcatca taaacatctc ggcgatatca tccgggtcga tgatgcggat ttcgccgggg
    21661 tttagcccca tatcagtagc gtcaacaatc agtaaccgcg tagggcgcag ttcacggatg
    21721 gcgacgatgt cgttttccgg tgcactaccg ccatcaatca ccacccagtt acctttcggc
    21781 gcggcggcgc acttttccgc cagcagcggg ccagcgccat catcgcccat catgctattg
    21841 ccaacacaga gtaaaacgtc agtcacgcag tctcctcacc atcaaataga tggcgttttc
    21901 ctgatgaata tcatgcagca tacttagcag cattgtactc cattcctgct gctgcgaagt
    21961 ttgcgctgcc cgggctttat cgaatgcgtt ggccagcatc ggcacatggt taatgtcgat
    22021 gacgatctca ccatatttcg gcacgccttc cattttgcgg cgggcttcgc tgcccgcctc
    22081 cagagtggca atccatgcca gatattcatc ccacgggcag gtgagcgccg cttccaggca
    22141 atcgataacc ccaaggtggt gaccaatcgc caggctgtaa tagaccacct gctgcgcctc
    22201 ggcgggcgtg gcatcgttct catcaataaa tttacggctc agttgactga acaccacctt
    22261 ttcactcatc ggatacgcgc ctcttctaca acatgattca gatggctgac aatctcgttc
    22321 agacgcggat cgttttccgc ttccagccag cgtgcaacct gttcttcgcc ctgccctaac
    22381 tgtgtcagga agtcatcggc aatctgacga ccgtaacgat aacctgccag gcggcgtgct
    22441 tcgcgatcca ctttcacacg cagcggctgc accatatcgc catgcaggat ttccgccggt
    22501 tgttcatcct gctcacccgg gccacgggcg tgaattttct gctccagcag gccgagcgcc
    22561 attgcaaagc cgtacagcgt ggcggcaggc gttggcgggc aacccggaat gtagacatct
    22621 accgggatga ttttatccgt accgccccac acgcagtaga gatcgtggaa aataccaccg
    22681 ctgttaccgc aggcaccgta ggagatacaa attttcgggt ccggcgcgga ctgccacgca
    22741 cgcagcgcag gggatcgcat tgcacgggtg accgcgccgg taaacagtaa aatatccgca
    22801 tgacgcggtg aaggaacgac tttaatgccg aagcgttctg catcaaacag cggcgataaa
    22861 gtggcgaaaa tttcgatttc gcagccgttg cagccgccgc agtccacgcg gtaaacgtag
    22921 gcagaacgtt tgattttttt cagtaacgac gccttcatgc tggcgatgga ttcatccacc
    22981 gtcatcggga ccggaatgcc gttggcgtca cgggggccta ataaattgct catcagatgg
    23041 cctctttcat atggcgagtc agttcaatac ggtcggacgg caccaggcat ttctggcgct
    23101 tacattccgg gcaagtctca aagctttcgc ggtggttttc cgcgcggctg tcaccattgt
    23161 gcttaagcag cgcaatggcg tagtcgatct ctttctggac ggcgaaagga cgattgcaga
    23221 cgcgacagct gcacagcaca aaacgggact gttgcaggaa atcttctttc ttccacaccg
    23281 ccagttcgta ctcttgcgac agtttgatcg ctgccgtcgg gcagacttct tcgcagcgtc
    23341 cacagaagat gcagcgcccg agattaaact cccacgcaag ctctcctgtg gcgaggtcag
    23401 tttcaaccgt taaggcgttt gacgggcagg cattgacgca ggccgcgcag ccgatgcact
    23461 gctgcgggtt ctgctctggc ttaccacgga agtttttatc aaccgcaatc ggctccagcg
    23521 gataagacga ggtcgccgtg ccggttttga tgactttttt gataaaggta aacatggcga
    23581 ttccttattt cagcggcgag tttttacgct caatgctgta acgctcgagt tctttgtacg
    23641 gcaccacttt gctcttcttc ttacgcacat cgaccacggt catgcggtcg gtacaggagt
    23701 agcaagggtc caggctaccg ataatcagcg gcgcatcgga aacggtgttg ccgcgcagca
    23761 tgtagcgcag ggtcggccag ttcgcgtagg tcgcggcacg gcagcgccag cggtacagct
    23821 tctggttgtc gccggtcatg ctccagtgga tatcgtcgcc gcgcggcgct tcggcaaagc
    23881 ccagcgcgaa acggtgcgga atgtaggtaa agccttccac catcagcggg ccgcccggca
    23941 ggttatccag accgtagtcg atcatgttca gcgcggtata gacttcattg atacgcactt
    24001 tcagacggga aataacgtcg cagccctgct cgctgtgcac ttccatcggc agcaggccat
    24061 aaccaacaaa cgggtgatcg gcgcgggtat cgcgagcgtg accgctggca cggaccatcg
    24121 ggccaacgtt actgaagtcg cgggcaattt ccgggtccag acgaccaatg ccgacggtgc
    24181 gctgttccat gttcggcgta ctcagcagca catccaccag ctcctgcact tcacgacgca
    24241 tctgttgcgc cagctggcgg gtctggatca tgtcatcttt cagcagatcg cgacgaatcc
    24301 cgccgatcag gttcaggccg taggttttac gcgcaccggt aaggatctct gccattttca
    24361 tggaggtttc acgcacgcgg aagaactgca taaagccgga gtcgaagccg gtaaagtgac
    24421 aggccaggcc gaggttgagc agatgcgagt gcaggcgttc cacctccagc agaatggcgc
    24481 ggatcatctg cgcacgttct ggcacctgaa tacccatcgc gttttccacc gacgtggtgt
    24541 aggcggtgct gtgagcaaag ccgcagatcc cgcacacacg gtcagagagg aaggtcactt
    24601 cgttgtaacc catacgggtt tccgccagtt tttccatgcc gcgatggacg tagaacaggc
    24661 ggtagtcggc gtcgataatg ttttcgccat cgacgaacag acggaagtgc cccggttcgt
    24721 cagaagtgac gtgcagcgga ccaatcggca cgacgttgtt tttcttgtcg cccagttcat
    24781 tgatgaactc gtaggtttca gcatcggtag tcggtgccgg acgctgacga taatccatgc
    24841 tgtctttacg cagcggataa agttcatccg gccagtcatc cggcagcacc agacgacgtt
    24901 catccggcag accaaccgga atcaaaccgt acatatcgcg cacttcgcgc tcgccccaca
    24961 ccgccgccgg aacgcgcggc gtcacggacg gatactccgg tttgttggcg tcaacttcga
    25021 cgcgaaccgt tacccaacac ttggtgccct tctccatcga cagcacgtaa taaacggcgt
    25081 aatgaccatt cagtttgcgt tcgtcgttac caaacagcac cgacagccag ccaccctgct
    25141 tgtagtagag aaactccacc acttccggca ggtagttcac cttcacggtc acggtcagct
    25201 gatctttggt ctgccaggcg tggtccagca cgacgcccgg aaatgcctca ttcagcgcgg
    25261 cgagataatg ttgacctaat ttttcttcag acatgctcaa actctcttta atcacgccgc
    25321 cagcaaggag acgaacgcta aaaatgcaaa gccaaaccca gcccaggtaa tgcgcggagt
    25381 aatatcaaga cgcagacggg ccatgctgtt ttcgaacagc gcgataacca ggacgccgac
    25441 gaccagtttg acgatggcaa tcaccagcgc cagcagcagc ccaccggcgg tgaaggtttc
    25501 catctgtccc cacgggataa atacgccgac gaacatctgc agcaccacca gctgtttcag
    25561 gctgataccc catttcatga cgccaaagcc gctgccgctg tattcagaga gcgggccttc
    25621 ctgcagctcc tgctcggctt ccgccaggtc gaacggcagt ttgcccattt cgataaaggt
    25681 ggcgaacgca caggcacaaa gcgccagtac cagcgggatg ctctggctca gcggccagtg
    25741 ataaacggtg tcggtgatgt tgctgatgtt ggtggaaccg gcaacctgtg cggcaaccca
    25801 cagaccaagc agcagcatcg gttcgaccag cacgccaagc atcgcttcac ggctcgcgcc
    25861 gatagcggta aacgggctac cggtatccag accagaaatg gcaaagaaga aacgcgcgat
    25921 ggcaaagaga tacagtaagg tgattaaatc acccagttgc ggcagcggag aaccgacggt
    25981 caccaccggc agcgcagtag cgatagtcag catgacgccc accatcacat acggcgtcag
    26041 gcggaacacc cagccggagg catccgggcc aacgctctgg cggcccagca gtttgaaaat
    26101 gtcacgatac tcctgcaaca cacccggccc acgacggtta tgcagacggg cgcgcgccac
    26161 gcgggtaata ccggagagca gcggcgcaac ggcaaataac accagcgcct gaattaacgg
    26221 atataaaaca ctcattctca ggctcctcgt gaaacaataa tcaccaccag taccgccagt
    26281 tcaaccagcg ccatccggcg gaacagcaac gcactacctt cgcactgcca gcccggcacc
    26341 agcgacaccg gattcagcca tttgcgcagt ttcagcactg gcgcaaacgc ctgtttcacc
    26401 ggcatggcaa aaccgtgagc ggtaatcacc attgattttt catggtcgta gccgcacacc
    26461 caggccgcac cgcgggaacg tgacggcagg cggtcgcctt tgcaaatcgc cataatgatg
    26521 aatggcagca gcgggcaggc aatcagcagc aatgtgatca ttggttgaga aacggtggtg
    26581 ttagcaggct ccagaggcag aggtacagca gcagagagca tcggcagcag ccacggcgca
    26641 gcaacaccgc caattacgca gcaaatcgcc agtgccacta cgcttacgct catcaggagc
    26701 ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg cgccgaggaa cgtgacgcca
    26761 tagactttcg ccatacacat caccgccagc gcaccggtaa ttgccagacc cacagcgagc
    26821 agcggcccca gcagacgggc aacaaacgcg ccgctattgc tcagtttgaa gaaggattga
    26881 tagataaccc attccccggc aaacccgttc agcggcggca gcgcagccat tgccattaac
    26941 ccgactaaca tggcgatgga gataaccggc attttcttgc cgataccacc gagtttttcg
    27001 atatcgcgat gaccggtacg gaaccagacg ctccccgctc cgaggaacag tacgcttttg
    27061 aacaggctat ggttaagcag atggtacaga ccaccgacca ggccaagagc aatcagtgcc
    27121 ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc ccagcagaat aatgccgata
    27181 ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gttccatcaa cgcatacaga
    27241 ccaccgacga acgcggtgat catgccgagc accagcagcg cgatccccca ccacagcggt
    27301 gcattaccgc caagcagtga cagggttaac atgcccagca ggccaatttt catgactacc
    27361 gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg cacccagccg
    27421 tgcagcggaa taatcccggc cagcaggcca aagccaatca caccgagcag ccagatatcg
    27481 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccataa
    27541 cgctgccaca gcagccagca agcaatcgcc agcagcagag tgccaagacg ccccagcgca
    27601 aaccacagtt tgccctcttt gctgttgctg gtaaggaaca ccgcacacaa ggccatgact
    27661 tcagccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac ggcggcagcc
    27721 atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg
    27781 ttgtagaggc tgacaaacag accgcacaga ccgagcgtaa tcagccaaat cgcgttaagc
    27841 ggagagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca
    27901 gtcagcacag taaagcccgc ggctgccgta tacaggctac caaccgcgcc gccaattccg
    27961 gctatccagc cgcttagcgc cttttgaaaa gaaaagagaa atgccagaac agcggcggcg
    28021 acaaaccacg ccacgccgct attgatcagg gaaattgcgc tcatttagcc tctccacttt
    28081 gagcctgctg aaacaaggtg agatcgccaa agtccgtgtt aaaggtcagc tcacgcttac
    28141 gtttgctaac gcgggcgata tcggtgttat ccaccagatg cagggctttg gtcgggcaca
    28201 tccgcacgca ggccggacct tgttcatcaa agctacaaag gtcacatttg acggcgatcg
    28261 cgcgaatacc cggcacccag tcaagcagcg tactgatacg cgccggagca ggcggtgccg
    28321 gtggcgcttt cggggtattg gcatttgcag gaatatccag cggacggctg ccggaaaatt
    28381 caattgcgcc aaacgggcag gcgatgccgc acagcttgca gcttacgcac aggctttcat
    28441 tcaactgcac ggccccatca acgcgggtga tggcgttaac cgggcagacc accgcgcagg
    28501 gtgcatcttc acagtggtga cagagctgcg gcgcagattc tttttcattc agcatcactc
    28561 tcaggcgcgg cattgattgc aggccgtgct ggcgatgcgt ctctgaacag gcggcctcac
    28621 aagtgtggca gccgatacag agcgtggagt cagcaattac aaaacgattc accaggcatt
    28681 cctcaggtga ttgtcatttt tgacgaaaac atgccgatga aatgtcattt tcgacactca
    28741 tcgacacgcc catccccaaa caggcgtaac gcctgcaaaa cgggcaaagc ctcagctcat
    28801 gctgccgggc tttgtccctt taccagttgg cttaaattca ccggcacatt gttttcaatg
    28861 gcggtgtata agctgtcgta aacgccagcg attttttcga gatagtgttc cagaacctgt
    28921 tcgcgatcgc ggatgttgac cttgccatca atcatgccgt caatgctcag ttgcgcctgc
    28981 gcaatccgct gtttgtcttc gccatctttt gtttcgacgt aatactcgat ggtgtggctg
    29041 ttaaagccat ccgccagggt gacgtaaata cgaaaatgtt caaaaaggac gaaacagagt
    29101 tctttgtccg gcgcgcagct catggcgtga tgcaggcgcg ctttcgataa cgtgatcccc
    29161 tgaaccagcg aattgcagta gatgtgccac tggtcctgta ggcgacgatg ccgctgtgcg
    29221 atgtaatcgg ctttctcgct tatttcccaa atagtcattg tcaggttacc cgtttaacag
    29281 agatgccagc tttaagcatt ttttgtgcca acttttaatt tattgttatt aaagagattt
    29341 ttaagataaa gatgaatttc gtcgccgtgt cgacgtgtca tttcgacatc atcgacatta
    29401 ttcaccgcag ggataatcaa cactggcaca attattgctt gtagctggca atagttaatg
    29461 ggaggcgata tgcacgaaat aaccctctgc caacgggcac tggaattgat cgaacagcag
    29521 gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc tcaaaattgg cgcattttct
    29581 tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg tttgccgcgg cagcgtggcg
    29641 gaaggttgta aactgcacct cgaagaacaa gaggccgaat gctggtgtga aacatgccaa
    29701 cagtatgtga cgctactgac ccagcgcgtc cgccgctgtc cacagtgtca tggtgacatg
    29761 ctacagattg tggcagacga cggtttacag attcggcgga tagaaataga ccaggagtga
    29821 gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctgt atatcgaggg tgatgaacat
    29881 aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg cggcacgccc gaagatgaaa
    29941 atcaccggca ttaaagcgcc tgaatttacc cccagccaga ctgaagaagg cgacctgcat
    30001 tacggtcatg gcgaagcggg cactcacgcg ccgggcatga gccagcgtcg gatgctggaa
    30061 gtcgaaattg acgtgctgga caaaaataac cgtctggctg aacgcaaccg cgcgcgcttt
    30121 gctgcccgca agcaactggt gctcaacctg gtttccagcc ctggttccgg taaaaccacc
    30181 ctgctgacgg aaaccttaat gcgcctgaaa gacagcgttc cgtgcgcagt tattgaaggc
    30241 gatcagcaaa ccgtgaacga tgccgcacgc attcgcgcta ccggcacacc agcgattcag
    30301 gtgaacactg gtaaaggctg ccaccttgac gcacagatga ttgccgacgc cgcaccgcgt
    30361 ctgccactgg acgataacgg tattctgttt atcgaaaacg ttggcaacct cgtatgcccg
    30421 gccagcttcg atctcggtga aaaacacaaa gtagcggtcc tttccgttac cgaaggtgaa
    30481 gacaaaccgc tgaaatatcc gcatatgttt gccgccgcct cgctgatgct gctcaacaaa
    30541 gttgaccttt tgccgtatct caactttgac gttgagaagt gcatcgcctg cgcacgcgaa
    30601 gtcaatccag aaattgaaat catccttatt tccgccacca gcggcgaagg gatggaccag
    30661 tggctgaact ggctggagac acagcgatgt gcataggcgt tcccggccag atccgcacca
    30721 ttgacggcaa ccaggcgaaa gtcgacgtct gcggcattca gcgcgatgtc gatttaacgt
    30781 tagtcggcag ctgcgataaa aacggtcagc cgcgcgtggg ccagtgggta ctggttcacg
    30841 ttggctttgc catgagcgta attaatgaag ccgaagcacg cgacactctc gacgccttac
    30901 aaaacatgtt tgacgttgag ccggatgtcg gcgcgctgtt gtatggcgag gaaaaataat
    30961 gcgttttgtt gatgaatatc gcgcgccgga acaggtgatg cagttaattg agcatctgcg
    31021 cgaacgtgct tcacatctct cttacaccgc cgaacgccct ctgcggatta tggaagtgtg
    31081 tggcggtcat acccacgcta tctttaaatt cggcctcgac cagttactgc cggaaaacgt
    31141 tgagtttatc cacggtccgg gttgtccggt gtgtgtactg ccgatgggta gaatcgacac
    31201 ctgcgtggag attgccagcc atccggaagt catcttctgt acctttggcg acgccatgcg
    31261 cgtaccaggg aaacagggat cgctgttgca ggcaaaagca cgcggtgccg atgtgcgcat
    31321 cgtttactcg ccgatggatg cgttgaaact ggcgcaggag aatccaaccc gtaaagtcgt
    31381 attcttcggc ttaggttttg aaaccaccat gccgaccacc gccatcaccc tgcaacaggc
    31441 gaaagcccgc gatgtgcaga atttttactt cttctgccag catattacgc ttatcccgac
    31501 gctgcgcagt ttgctggaac agccggataa cggtatcgac gcgttcctcg cgccgggcca
    31561 cgttagtatg gttatcggca ctgatgccta taattttatc gccagcgatt ttcatcgtcc
    31621 gctggtggtc gctggtttcg aaccccttga tctactacaa ggcgtggtca tgctggtgga
    31681 gcagaaaata gcggcccaca gcaaggtaga gaatcagtat cgtcgagtgg taccggatgc
    31741 cggtaacctg ctggcgcaac aggcgattgc cgatgtgttc tgtgtcaacg gcgacagcga
    31801 atggcgcggc ttaggcgtga ttgaatcttc tggcgtacac ctgacgccgg attatcaacg
    31861 attcgatgcc gaagcacatt tccgcccggc accgcagcag gtctgcgatg acccgcgcgc
    31921 acgttgtggt gaagtattaa cgggcaaatg taagccgcat caatgcccgc tgtttggtaa
    31981 cacctgtaat cctcaaaccg catttggtgc actgatggtc tcctccgaag gagcgtgcgc
    32041 cgcgtggtat cagtatcgtc agcaggagag tgaagcgtga ataatatcca actcgcccac
    32101 ggcagcggcg gccaggcgat gcagcaatta atcaacagcc tgtttatgga agcctttgcc
    32161 aacccgtggc tggcagagca ggaagatcag gcacgtcttg atctggcgca gctggtagcg
    32221 gaaggcgacc gtctggcgtt ctccaccgac agttacgtca ttgacccgct gttctttcct
    32281 ggcggtaata tcggcaagct ggcgatttgc ggcactgcga atgacgttgc ggtcagtggc
    32341 gctattccgc gctatctctc ctgtggcttt atcctcgaag aaggattgcc gatggagaca
    32401 ctgaaagccg tagtgaccag catggcagaa accgcgcgca cggcaggcat tgccatcgtt
    32461 actggcgata ctaaagtggt gcagcgcggc gcggcagata aactgtttat caacaccgct
    32521 ggcatgggcg caattccggc gaatattcat tggggcgcac agacgctaac cgcaggcgat
    32581 gtgttgctgg tgagcggcac gctcggcgac cacggggcga ctatccttaa cctgcgtgag
    32641 cagctggggc tggatggcga actggtcagc gactgcgcgg tgctgacgcc gcttattcag
    32701 acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg ccacccgtgg tggtgtaaat
    32761 gcggtggttc atgagttcgc ggcagcctgc ggttgcggta ttgaaatttc tgaatcagcg
    32821 ctgccggtta aacctgccgt gcgcggcgtt tgcgaattgc tgggactgga cgccctgaac
    32881 tttgccaacg aaggcaaact ggtgatcgcc gttgaacgca acgcggcaga gcaagtgctg
    32941 gctgcgttac attcccatcc actggggaaa gacgcggcgc tgattggtga agtggtggaa
    33001 cgtaaaggtg ttcgtcttgc cggtctgtat ggcgtgaaac gaaccctcga tttaccacac
    33061 gccgaaccgc ttccgcgtat atgctaataa aattctaaat ctcctatagt tagtcaatga
    33121 ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa atgtcatata caccgatgag
    33181 tgatctcgga cagcaagggt tgttcgacat cactcggaca ctattgcagc agcccgatct
    33241 ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg ccgacaacgc
    33301 ggctattgtg ttgtggcaag cgcagactca acgtgcgtct tattacgcat cgcgtgaaaa
    33361 agacaccccc attaaatatg aagacgaaac tgttctggca cacggtccgg tacgcagcat
    33421 tttgtcgcgc cctgatacgc tgcattgcag ttacgaagaa ttttgtgaaa cctggccgca
    33481 gctggccgca ggtgggctat acccaaaatt tggtcactat tgcctgatgc cactggcggc
    33541 agaagggcat atttttggtg gctgtgaatt tattcgttat gacgatcgcc cctggagcga
    33601 aaaagagttc aatcgtctgc aaacatttac gcagatcgtt tctgtcgtca ccgaacaaat
    33661 ccagagccgc gtcgtcaaca atgtcgacta tgagttgtta tgccgggagc gcgataactt
    33721 ccgcatcctg gttgccatta ccaacgcggt gctttcccgc ctggatatgg acgaactggt
    33781 cagcgaagtc gccaaagaaa tccattacta tttcgacatt gacgatatca gtatcgtctt
    33841 acgcagccac cgtaaaaaca aactcaacat ctactccact cactatcttg ataaacaaca
    33901 tcccgcccac gaacagagcg aagtcgatga agccggaacc ctcaccgaac gcgtgttcaa
    33961 aagtaaagag atgctgctga tcaatctcca cgagcgggac gatttagccc cctatgaacg
    34021 catgttgttc gacacctggg gcaaccagat tcaaaccttg tgcctgttac cgctgatgtc
    34081 tggcgacacc atgctgggcg tgctgaaact ggcgcaatgc gaagagaaag tgtttaccac
    34141 taccaatctg aatttactgc gccagattgc cgaacgtgtg gcaatcgctg tcgataacgc
    34201 cctcgcctat caggaaatcc atcgtctgaa agaacggctg gttgatgaaa acctcgccct
    34261 gaccgagcag ctcaacaatg ttgatagtga atttggcgag attattggcc gcagcgaagc
    34321 catgtacagc gtgcttaaac aagttgaaat ggtggcgcaa agtgacagta ccgtgctgat
    34381 cctcggtgaa actggcacgg gtaaagagct gattgcccgt gcgatccata atctcagtgg
    34441 gcgtaataat cgccgcatgg tcaaaatgaa ctgcgcggcg atgcctgccg gattgctgga
    34501 aagcgatctg tttggtcatg agcgtggggc ttttaccggt gccagcgccc agcgcattgg
    34561 tcgttttgaa ctggcggata aaagctccct gttcctcgac gaagtgggcg atatgccact
    34621 ggagttacag ccgaagttgc tgcgtgtatt gcaggaacag gagtttgaac gtctcggcag
    34681 caacaaaatc attcagacgg acgtgcgtct aatcgccgcg actaaccgcg atctgaaaaa
    34741 aatggtcgcc gaccgagagt tccgtagcga tctctattac cgcctgaacg tattcccgat
    34801 tcacctgccg ccactacgcg agcgtccgga agatattccg ctgctggcga aagcctttac
    34861 cttcaaaatt gcccgtcgtc tggggcgcaa tatcgacagc attcctgccg agacgctgcg
    34921 caccttgagc aacatggagt ggccgggtaa cgtacgcgaa ctggaaaacg tcattgagcg
    34981 cgcggtattg ctaacacgcg gtaacgtgct gcagctgtca ttgccagata ttgttttacc
    35041 ggaacctgaa acgccgcctg ccgcaacggt tgtcgcccag gagggcgaag atgaatatca
    35101 gttgattgtt cgcgtgctga aagaaaccaa cggcgtggtt gccgggccta aaggcgctgc
    35161 gcaacgtctg gggctgaaac gcacgaccct gctgtcacgg atgaagcggc tgggaattga
    35221 taaatcggca ttgatttaac tgcaaattgc cggacagatc tgcctgtccg gcatactatt
    35281 cacgaggttt tttcggacga tatttttccg gcagttctgg caccggacgc ttgtcatcga
    35341 tgagatgacg cacggttaag atcggatgac gccacagcat tctcggcccg gcccaacgca
    35401 taatctgttt catctcttca cgctttgcag gctggtaaca gtgcaccgga cactgcttac
    35461 aggctggttt ctcttcgccg aacacacatt tatccagccg cttttgcgcg taaacaaaca
    35521 acgcctcgta atgctccggc tccgctgacg cctgcgggca tttcgcttga taaagatcga
    35581 tcattttttt aatcgtcagt ttttcacgag agatacgctt gccggacatg ctgcctccac
    35641 ctcattaaga tgtatttata ttacatctta atcttaaagg gcactatgac tctaaagaag
    35701 aaaggttcgc caaccgacgc aattttgcgt acttgcttca taagcatcac gcaaaagctg
    35761 caaaacagca tctttcccgg aaccagcatc aagaactcgc cgttcgcttc ttcccctgaa
    35821 atgattaact ccggtatcat gtgcgcctta tgtgattaca acgaaaataa aaaccatcac
    35881 accccattta atatcaggga accggacata accccatgag tgcaatagaa aatttcgacg
    35941 cccatacgcc catgatgcag cagtatctca agctgaaagc ccagcatccc gagatcctgc
    36001 tgttttaccg gatgggggat ttttatgaac tgttttatga cgacgcaaaa cgcgcgtcgc
    36061 aactgctgga tatttcactg accaaacgcg gtgcttcggc gggagagccg atcccgatgg
    36121 cggggattcc ctaccatgcg gtggaaaact atctcgccaa actggtgaat cagggagagt
    36181 ccgttgccat ctgcgaacaa attggcgatc cggcgaccag caaaggtccg gttgagcgca
    36241 aagttgtgcg tatcgttacg ccaggcacca tcagcgatga agccctgtta caggagcgtc
    36301 aggacaacct gctggcggct atctggcagg acagcaaagg tttcggctac gcgacgctgg
    36361 atatcagctc cgggcgtttt cgcctgagcg aaccggccga ccgcgaaacg atggcggcag
    36421 agctgcaacg cactaatccg gcggaactgc tgtatgcaga agatttcgcc gagatgtcgc
    36481 tgattgaagg ccgtcgcggc ctgcgccgtc gcccgctgtg ggagtttgaa atcgacaccg
    36541 cgcgccagca gttgaatctg caatttggca cccgcgatct ggtcggtttt ggcgtcgaga
    36601 acgcgccgcg cggactttgt gctgccggtt gtctgttgca gtatgcgaaa gatacccaac
    36661 gcacgaccct gccgcatatt cgttctatca ccatggaacg tgagcaggac agcatcatta
    36721 tggatgccgc gacacgtcgt aacctggaaa ttactcagaa cctggcgggc ggtgcggaaa
    36781 atacgctggc ttctgtgctc gactgcaccg tcacgccgat gggtagtcgt atgctgaaac
    36841 gctggctgca tatgccagtg cgcgataccc gcgtgttgct tgagcgccag caaactattg
    36901 gcgcattgca ggatttcacc gccgagttgc agccggtact gcgtcaggtc ggcgacctgg
    36961 aacgtattct ggcacgtctg gctttacgaa ctgctcgccc acgcgatctg gcccgtatgc
    37021 gccacgcttt ccagcaactg ccggagctgc gtgcgcagtt agaaactgtc gatagtgcac
    37081 cggtacaggc gctacgtgag aagatgggcg agtttgccga gctgcgcgat ctgctggagc
    37141 gagcaatcat cgacacaccg ccggtgctgg tacgcgacgg tggtgttatc gcatcgggct
    37201 ataacgaaga gctggatgag tggcgcgcgc tggctgacgg cgcgaccgat tatctggagc
    37261 gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac gctgaaagtt ggctttaatg
    37321 cggtgcacgg ctactacatt caaatcagcc gtgggcaaag ccatctggca cccatcaact
    37381 acatgcgtcg ccagacgctg aaaaacgccg agcgctacat cattccagag ctaaaagagt
    37441 acgaagataa agttctcacc tcaaaaggca aagcactggc tctggaaaaa cagctttatg
    37501 aagaactgtt cgacctgctg ttgccgcatc tggaagcgtt gcaacagagc gcgagcgcgc
    37561 tggcggaact cgacgtgctg gtgaacctgg cggaacgggc ctataccctg aactacacct
    37621 gcccgacctt tattgataaa ccgggcattc gcattaccga aggccgccat ccggtggttg
    37681 aacaggtgct gaatgagccg tttatcgcca acccgctgaa tctgtcgccg cagcgtcgca
    37741 tgttgatcat caccggtccg aacatgggcg gtaaaagtac ctatatgcgc cagaccgcgt
    37801 tgattgcgct aatggcctat atcggcagct acgtaccggc gcaaaaagtc gagattggcc
    37861 cgattgaccg tatctttacc cgcgtaggcg cggcagatga cctggcttcc gggcgttcga
    37921 cctttatggt ggagatgacc gaaaccgcca atattttaca taatgccacc gaatatagtc
    37981 tggtattgat ggatgagatc gggcgtggaa cgtccactta cgatggtctg tcgctggcgt
    38041 gggcgtgcgc ggaaaatctg gcgaataaga ttaaggcatt gacgctgttt gccacccact
    38101 atttcgagct gacacagtta ccggagaaaa tggaaggcgt cgccaacgtg catctcgatg
    38161 cactggagca cggcgacacc attgccttta tgcacagcgt gcaggatggc gcggcgagca
    38221 aaagctacgg cctggcggtt gctgctctgg cgggcgtacc gaaagaggtt attaagcgcg
    38281 cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa cgccgccgct acgcaagtgg
    38341 atggtacgca aatgtctttg ctgtccgtac cagaagaaac ttcgcctgcg gtcgaggcac
    38401 tggaaaacct ggacccggat tcactgactc cgcgtcaggc gctggagtgg atttatcgct
    38461 tgaagagtct ggtgtaataa aaattcccga tagtcttttg ctatcgggaa tattaacgac
    38521 aactgacgaa tcaaataaaa ataccctgta taataggaaa gcttatttta cagggtaaaa
    38581 ccatgccatc tacacgctat caaaaaatca atgcccatca ctatcgccat atatgggtcg
    38641 ttggtgatat tcatggtgaa tatcagttat tacaatcccg cttacatcaa ctctcttttt
    38701 tccccgaaac cgacttactt atttctgtcg gcgataatat tgatcgtggg ccggagagtc
    38761 ttgacgtcct gcgcctgcta aaccagccct ggtttatctc cgttaaaggc aaccacgaag
    38821 caatggcgct ggatgcgttc gcgaccggcg atggcaatat gtggcttgcc agcggtggtg
    38881 actggttttt cgatttaaat gattcagagc aaaaagaagc tacagatctg ttgctgaaat
    38941 tccatcacct tccacatatt attgaaatca ctaacaacaa cataaaatat gtcatcgcac
    39001 atgcagatta tccggggagt gaatatctct ttggtaaaga aatagcggag agcgaattac
    39061 tctggcctgt tgatcgtgtg cagaaatcgc ttaatggcga gttacaacaa ataaacggcg
    39121 ctgattattt tatatttgga catatgatgt ttgataacat tcagacgttc gctaaccaga
    39181 tttatattga taccggatcg ccgaaaagtg ggcggctgtc attttataaa ataaggtagt
    39241 ctcatgcttc ttctgtgaag catgagtaac cagatgttat tgcaggccat tattcatttt
    39301 tcgctaccag caaagagaga tcctgcttcg ccagcgcgcg actggcactc tccggcaaac
    39361 cgtcgtctgt aataatctga tcaaactcgc ttaatggtaa cgccagccat gtcgccacct
    39421 gaccatattt cgtcgcatca cagaccaaaa ctcgctggcg gctggcactg gcaatcgccc
    39481 gtttcaccgt gactttatct tccgctggcg tagaaatccc ccgcacactc catgacgatg
    39541 cagaaataaa agcctgatca atcatcaggc tgcgcagcat ggtcgcagcg gcttccccga
    39601 cacaggaacg gttttcccga cacactgcac cgccagtgtg aataattgtg caattactgt
    39661 tgtcgagcaa gtagttcgca ataacgaaat cgtttgtgac cacagtcagt gactccatgt
    39721 gaatcagatg ctgtgctatc gctaacgtgg tcgttcccgc atccagatag atacaacttc
    39781 ccggctgaac aagacttgcc gccagcttgc caatagccgc tttttgcgtc attgccagcg
    39841 cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc cggagactgg acgcctccgg
    39901 acaccagcac aacggctccc tgctgctcca gtttttgtaa atcccgacga atggtcatat
    39961 gtgacacatt cattctgtcc gttagttcag caatactgac aatgcctttt tcagctacca
    40021 tctcaaggat gatttggcga cgctctacgg gtatcaactt ttgctccttc ctttgtcctg
    40081 ctgacattct acgctatttg cctgcgaaac gtgcgcggcg caactaacgc ttagttcaca
    40141 taaaataaca cacaatgtta atttatgtga atcagctcac catatcgtta tcttccagcg
    40201 cttatattca caatatcaaa caaaatatca cttaaattaa caaggagagc agatgaaaac
    40261 gggatctgag tttcatgtcg gtatcgttgg cttagggtca atgggaatgg gagcagcact
    40321 gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac ctgaacagca atgcctgcgc
    40381 tacgttgaaa gaggcaggtg cttgcggggt ttctgataac gccgcgacgt ttgccgaaaa
    40441 actggacgct ctgctggtgc tggtggtcaa tgcggcccag gttaaacagg tgctgtttgg
    40501 tgaaacaggc gttgcacaac atctgaaacc cggtacggca gtaatggttt cttccactat
    40561 cgctagtgct gatgcgcaag aaattgctac cgctctggct ggattcgatc tggaaatgct
    40621 ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac ggtgaaatga ctgtcatggc
    40681 ctccggtagc gatattgcct ttgaacgact ggcacccgtg ctggaagccg ttgccggaaa
    40741 agtttatcgc ataggtgcag aaccgggact aggttcgacc gtaaaaatta ttcaccagtt
    40801 gttagcgggc gtacatattg ctgccggagc cgaagcgatg gcacttgcag cccgtgcggg
    40861 gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc gccggaaatt cctggatgtt
    40921 cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc ccgcattcag ccgtcgatat
    40981 ttttgttaag gatcttggtc tggttgccga tacagccaaa gccctgcact tcccgctgcc
    41041 attggcctca acagcattga atatgttcac cagcgccagt aacgcaggtt acgggaaaga
    41101 agacgatagc gcagttatca agattttctc tggcatcact ctaccgggag cgaaatcatg
    41161 atcaagattg gcgttat
//
LOCUS       DAFATA010000027        40937 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFATA010000027 DAFATA010000000
VERSION     DAFATA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.027"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     repeat_region   32..426
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=32..60
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(523..816)
                     /gene="cas2"
                     /locus_tag="LGJ20_001906"
     CDS             complement(523..816)
                     /gene="cas2"
                     /locus_tag="LGJ20_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBK2385974.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(813..1736)
                     /gene="cas1e"
                     /locus_tag="LGJ20_001907"
     CDS             complement(813..1736)
                     /gene="cas1e"
                     /locus_tag="LGJ20_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HBK2385975.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPTIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(1733..2383)
                     /gene="cas6e"
                     /locus_tag="LGJ20_001908"
     CDS             complement(1733..2383)
                     /gene="cas6e"
                     /locus_tag="LGJ20_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBK2385976.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(2365..3111)
                     /gene="cas5e"
                     /locus_tag="LGJ20_001909"
     CDS             complement(2365..3111)
                     /gene="cas5e"
                     /locus_tag="LGJ20_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBK2385977.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDHFHDLKVSLPKLQDECWWEGEHDGLVVSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(3122..4177)
                     /gene="cas7e"
                     /locus_tag="LGJ20_001910"
     CDS             complement(3122..4177)
                     /gene="cas7e"
                     /locus_tag="LGJ20_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HBK2385978.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLSLLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(4192..4728)
                     /gene="casB"
                     /locus_tag="LGJ20_001911"
     CDS             complement(4192..4728)
                     /gene="casB"
                     /locus_tag="LGJ20_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBK2385979.1"
                     /translation="MSIVKDGHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVAANVKAIDERQPFAAQLAAVMSEGRFTCL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLADDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(4725..6287)
                     /gene="casA"
                     /locus_tag="LGJ20_001912"
     CDS             complement(4725..6287)
                     /gene="casA"
                     /locus_tag="LGJ20_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HBK2385980.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGIRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQDDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHRFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADGLLSKWNKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(6385..9084)
                     /locus_tag="LGJ20_001913"
     CDS             complement(6385..9084)
                     /locus_tag="LGJ20_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBK2385981.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGQPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPTSSLSLFHSRFAFSDRQRIETKTLARFGKE
                     GSSQRAGKALISTQVLEQSVDCDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPDEEWFGSAMRNSAYIYPDHGRIWLTQRVLRQQGAIQM
                     PHSARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYTTGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRQDPEMANVILVTDNESCGYSATEGLIGKVG"
     gene            complement(9278..9430)
                     /locus_tag="LGJ20_001914"
     CDS             complement(9278..9430)
                     /locus_tag="LGJ20_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBK2385982.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(9695..10429)
                     /gene="cysH"
                     /locus_tag="LGJ20_001915"
     CDS             complement(9695..10429)
                     /gene="cysH"
                     /locus_tag="LGJ20_001915"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBK2385983.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(10503..12215)
                     /gene="cysI"
                     /locus_tag="LGJ20_001916"
     CDS             complement(10503..12215)
                     /gene="cysI"
                     /locus_tag="LGJ20_001916"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBK2385984.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(12215..14014)
                     /gene="cysJ"
                     /locus_tag="LGJ20_001917"
     CDS             complement(12215..14014)
                     /gene="cysJ"
                     /locus_tag="LGJ20_001917"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBK2385985.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGELRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            14333..14695
                     /gene="queD"
                     /locus_tag="LGJ20_001918"
     CDS             14333..14695
                     /gene="queD"
                     /locus_tag="LGJ20_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBK2385986.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            14735..15289
                     /locus_tag="LGJ20_001919"
     CDS             14735..15289
                     /locus_tag="LGJ20_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBK2385987.1"
                     /translation="MLGCCVLDIWGMTWKTTATLLLLVPVLQAPLARYAWIYRSILES
                     VALMLKNNYDNMRNEMNHFAKHVIITGGGSNSDLFMQIFADMFNLPARRNAINGCASL
                     GAAINKAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRYDAMNKGIFKDLTKHNDVI
                     LKKSYEVMHGELGNVDSIQSWSNA"
     repeat_region   15928..16627
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=15989..16017
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(16967..17638)
                     /gene="queE"
                     /locus_tag="LGJ20_001920"
     CDS             complement(16967..17638)
                     /gene="queE"
                     /locus_tag="LGJ20_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBK2385988.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGSASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            17811..18413
                     /locus_tag="LGJ20_001921"
     CDS             17811..18413
                     /locus_tag="LGJ20_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HBK2385989.1"
                     /translation="MLRTFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSNHEKDVLEAVTLARSQANRASSDLQQTPGNEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNASAH"
     gene            18427..19332
                     /locus_tag="LGJ20_001922"
     CDS             18427..19332
                     /locus_tag="LGJ20_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBK2385990.1"
                     /translation="MKNLILLLVLICSSAFAVQVPVPEFAKYINDLTGTLTREEVSTL
                     TSQIKTLTQKSHAQLIVLVVETTGDETIEQYATRVFERWQPGHKNLDDGILLVVAWQD
                     HTVHIEIGYGLEGILTDAQSGQIIRNSIIPAFKKGDLAVGLQQGIGDIETRLSENDLV
                     NNTSPDHPLPFSGWWALLVWAIVLTFISARGYVKTLGVICFAAIILAFVLPIAGYSGS
                     WGVLATLLCFATPFLVVAIAFTPFGRKVRDSMRSANRPSRYERSNSASRHSSSSSSSS
                     SSSFNNDNFSGGGGSSGGGGASGRW"
     gene            19368..19730
                     /locus_tag="LGJ20_001923"
     CDS             19368..19730
                     /locus_tag="LGJ20_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2385991.1"
                     /translation="MSKILANHPRLSKFVFGLFGAAGMIWLNKAFQGDELFQLITSLL
                     ALDTFVYFCIYSLINPIPEANDFLWDYKVHQRKYSSNISHNDFVSNVRNSRKIVRILI
                     LVGITFINVIALLTKLKM"
     gene            complement(19786..21084)
                     /gene="eno"
                     /locus_tag="LGJ20_001924"
     CDS             complement(19786..21084)
                     /gene="eno"
                     /locus_tag="LGJ20_001924"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK2385992.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(21172..22809)
                     /gene="pyrG"
                     /locus_tag="LGJ20_001925"
     CDS             complement(21172..22809)
                     /gene="pyrG"
                     /locus_tag="LGJ20_001925"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBK2385993.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(23037..23828)
                     /gene="mazG"
                     /locus_tag="LGJ20_001926"
     CDS             complement(23037..23828)
                     /gene="mazG"
                     /locus_tag="LGJ20_001926"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBK2385994.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(23899..24234)
                     /gene="mazF"
                     /locus_tag="LGJ20_001927"
     CDS             complement(23899..24234)
                     /gene="mazF"
                     /locus_tag="LGJ20_001927"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBK2385995.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(24234..24482)
                     /gene="mazE"
                     /locus_tag="LGJ20_001928"
     CDS             complement(24234..24482)
                     /gene="mazE"
                     /locus_tag="LGJ20_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBK2385996.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(24560..26794)
                     /locus_tag="LGJ20_001929"
     CDS             complement(24560..26794)
                     /locus_tag="LGJ20_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBK2385997.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(26842..28143)
                     /gene="rlmD"
                     /locus_tag="LGJ20_001930"
     CDS             complement(26842..28143)
                     /gene="rlmD"
                     /locus_tag="LGJ20_001930"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK2385998.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            28200..30956
                     /gene="barA"
                     /locus_tag="LGJ20_001931"
     CDS             28200..30956
                     /gene="barA"
                     /locus_tag="LGJ20_001931"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBK2385999.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(31188..32528)
                     /gene="gudD"
                     /locus_tag="LGJ20_001932"
     CDS             complement(31188..32528)
                     /gene="gudD"
                     /locus_tag="LGJ20_001932"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBK2386000.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGRTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(32549..33889)
                     /locus_tag="LGJ20_001933"
     CDS             complement(32549..33889)
                     /locus_tag="LGJ20_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBK2386001.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHSAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHIGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIVTNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(33891..35243)
                     /gene="gudP"
                     /locus_tag="LGJ20_001934"
     CDS             complement(33891..35243)
                     /gene="gudP"
                     /locus_tag="LGJ20_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBK2386002.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(35678..36127)
                     /locus_tag="LGJ20_001935"
     CDS             complement(35678..36127)
                     /locus_tag="LGJ20_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK2386003.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(36145..36927)
                     /gene="truC"
                     /locus_tag="LGJ20_001936"
     CDS             complement(36145..36927)
                     /gene="truC"
                     /locus_tag="LGJ20_001936"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBK2386004.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(36927..37256)
                     /locus_tag="LGJ20_001937"
     CDS             complement(36927..37256)
                     /locus_tag="LGJ20_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBK2386005.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(37878..38423)
                     /gene="syd"
                     /locus_tag="LGJ20_001938"
     CDS             complement(37878..38423)
                     /gene="syd"
                     /locus_tag="LGJ20_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBK2386006.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            38491..39339
                     /gene="queF"
                     /locus_tag="LGJ20_001939"
     CDS             38491..39339
                     /gene="queF"
                     /locus_tag="LGJ20_001939"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBK2386007.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            39451..40815
                     /gene="ppnN"
                     /locus_tag="LGJ20_001940"
                     /gene_synonym="ygdH"
     CDS             39451..40815
                     /gene="ppnN"
                     /locus_tag="LGJ20_001940"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBK2386008.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT        10087 a        10830 c         9766 g        10254 t
ORIGIN      
        1 aaaaatgatg ctgtatttca ctattgggtt ccggtttatc cccgctggcg cggggaacac
       61 ccgttaaaat attcgcccgt tgtgtaagcg tacggtttat ccccgctggc gcggggaaca
      121 catctccagc gccttttcga cgcaacggtg cgccggttta tccccgctgg cgcggggaac
      181 actgaccgat tttattgatc gcccgataga cctgcggttt atccccgctg gcgcggggaa
      241 cacccagatt ccgctttctc taccttctct ttttccggtt tatccccgct ggcgcgggga
      301 acacgggttg tgcgccctgg tgggcgaaaa cggtggcggt ttatccccgc tggcgcgggg
      361 aacacatttt cctgatcgag ttttgagaat tttgtcgcgg tttatccccg ctggcgcggg
      421 gaacacacta agcatacata tctgttttta aacaaattta ttccacatca acaatctacc
      481 aactaaattc aaacatttcc ttatttttaa agaacacata acctattgat tatcaacagg
      541 aagaaaagaa accaaacgta acccatccaa atccaccgga atacgtctgt tttctcccca
      601 ggtctgaaat tcaaaacccg attcggtatt ggtcgcccag gccatcacca catttccgca
      661 accagccagt tgggtaattt gttgccagat catctcccga atacgttttg atgtatcacc
      721 aacatacaca ccggcacgca cttccagtag ccagattgcg agccgtccac gtaagcgcgg
      781 agggacattt tctgtaacaa ccacgaccat gctcatccgc cgcgcccccg gtgaccacta
      841 tcacccagcg tttcaggttc agggatggca ggcggtaaca tatccggcgc gggttgtggt
      901 ggttcaattt cacctgcagc aaggacttcc tcaattaacg gtattaattt gcccgttaac
      961 ttagtgctac ggaaaatatc gcgacaggct aatctgactt ctttatcagg ttctgcgggt
     1021 tgcctcgctg ctatttcaaa tgcctttggc acaaccgaat caaatttaat gatatcggct
     1081 atgtcataaa caaatgaaag cggtttgcca ctatgaataa atccaatagt gggcgcatat
     1141 cccgcggcta atactgccgc ttcagaaata ccgtacagac atgatgtggc agcactgatg
     1201 cagcgattca caacatcgcc tttttcccag tctttaggat cgtatttgcg accattccat
     1261 ttcacaccat attgtttcgc cagtaatgca taggtctggc gaacgcggga tccctcaatt
     1321 ccccgtagct gatccactga acggcgagct ggcggtggct cacgaaaacg taattcatac
     1381 attttgcgca ccaccttcag gcgtagatct tccgttaaag ccagctttgc ctggtagagt
     1441 aatttatctg cccgcgcccc tccgggttgt ccggaagagt aaacgcgaac gcccgcttca
     1501 ccgacccaga ccagcagtgt tcccaccgtg gcggccagat gcaccgccgc gtgggaaact
     1561 ctcgttcccg gttcgagcat aatgcaggcg accgatccca ccggaatgtg cgtgcggatc
     1621 ccggttttgt cgatcagcac gaaagcgccg tccagtacgt cgatttgacc gtactggagg
     1681 aagatcatag aggtgcgatc ttttaacggg atcggactca gtggtacaaa cgtcacgcct
     1741 ctgctccggg tttgatcagc atcagaccac agccgaacgc gcggcttttg ccatacccct
     1801 gactgagacg ttgcagaaat aacccgggat cggtgaccgt aagcatcccc gtataatcca
     1861 cgctactgaa ctggatcagt tgccgggagt tttcccgccg cagttgctgt tgtcggtagg
     1921 catcaaccga ggtatccagc agtgtaaaac cgcttctctc cccctgcgct gccagccagt
     1981 ccagcgccgc ctgttgttga tgcaaccaga catcacgccc ttccgcctgt cccctcacct
     2041 gccgtttcgc ctccatcagc agatcgtggc gcttgcccgc tttacaaatt gtgggattcg
     2101 cccgcaggtt gaaacaaagg gattgtccgg tacgcagctc cggagcaaat gaccggcatt
     2161 cgatagtgaa agtttcactt tccgccgggc gttcctgcga gaggacaaaa aagcgaaacg
     2221 caccctgaag ttcctcccga cgataaagaa attgcctttc tttgccgcca gggaagagat
     2281 cccacagcca ctgatgcatc acatattcac cgcgatccac cagatgcagc aactgcgcag
     2341 gcaaaagctg agcggtatgt aaggttattc ttgagaggta catggttcct ccttgctgag
     2401 ccacggcccc tgattgacgg tgcgttcccc aaacagccac tgctggcgat ttaaaggaac
     2461 atcccggcgg cgtaatattt tgctcacgac caggccgtcg tgctcccctt cccaccagca
     2521 ttcatcctga agtttcggga gtgagacttt caggtcgtgg aaatgatcct gatactgttg
     2581 gtatgcgtta cgtaaaacat cagacgcgtt gccttcgagc agtaacggcg caagtggtaa
     2641 tgccagcgga tgactttttc gccccagata aagaggaaaa actggatggc gtaaaccgcc
     2701 ctgcaactgt tcgaggctgt aaggcgcatc ggctgttgtt gccaccgcca ccatccacca
     2761 ggcgtcggtg tagtagtcgc gccgggagat aatcgcgctc agaagatcag ggtcgctcaa
     2821 ctcttcgcga cggctgaaat aacgcgcttt acgcacctct tttggcatct ggatcgtgtg
     2881 ataatcccgt gcccagcgcg ggttacggct ggcgcaaacc accagtgaat agtggcgatt
     2941 aaacgcgttt aagcgttcgg tatcatcacg ccgaatccct accccggcag ccagcagacc
     3001 cagcaatgct gagcgcgaag gcagttcatg ggtatgacgc acttcgccgg gggcatcgac
     3061 gccccaggat gccattggcc catgaagctg aaaaatcaga tattggctca taagccgccc
     3121 cttacgcgca gataaagtcc agcacgtcct tcatgcttcc ctgtttgttc atcacgtcaa
     3181 aacttgcgca ttcggtcttc tgttcgtaga ccgtattcat attttcgcga agcgttgtaa
     3241 tacgctgcac cgccacgtct aactgccggg tgccattaat gggttcatag aaggccgcag
     3301 ccagagaacg cggttgatcg gtgccttttt ccgccagcgc ccaggaagca taggcacggc
     3361 tggcaaagct attttgttta ccggttgggg aaactttaag cgcggcttcc gtaaaagcgc
     3421 gtatggtctg attagctaac gcttcgtcac cgccgaggtt ttctaccagc agatctttat
     3481 caatgcagat ataggtgtag aaaagtgctg aaccgaatcc ggtttctccg agatgcccgg
     3541 caccggcatc ttcagaggcc tggcgcaaat catcaacggc ggtgaaaaaa tcatcttcga
     3601 caatcgtttc actgacacca aacgcatgcg cgacctggca ggcggcttca acattaaact
     3661 caggtttatt cgccagcata cgaccaaaca tggcgatatc taccgccata cggtctttgc
     3721 gtaacagact gagatcttcc tcttttggcg cgcgcttttc ttctgccagt tgatgggcca
     3781 gcgcttttac ggcgtcaaat tctgccgggc tgatatggac taattgttca gtttcggcgt
     3841 tagtgagcgg atcttttggt tttttgtcgt ttttagcttt cccaagataa tctgcaattt
     3901 ttgccgccca ttcgatggct tttttatctt cgattccttt ctcaatcagg atagttgccg
     3961 cctcacgcgc gatacgccca ctgcgaatac caatatggcc cgccagcgcc tgttcaaaaa
     4021 gtgcagaagt gcgccacgca cgtttcagac tttgcgagga aacgcgcaga cgcgttgctc
     4081 cacccaacac cacggttttt ggcgcaccgg tgtcatcacg gttaaggttg gcagcagggt
     4141 aagcggttaa caaatgaagc tgaataaatg tcgtcatgag atagtccttt attaattttg
     4201 ttcgttatcg gcgtcaccgg cctggtaata ttccagcgcc cagcgaatac ggataaattc
     4261 ggttggcctc tgctggcggc ggtgatgatt gagcaggtcg tcgctctcct ggcaccagcg
     4321 gaaaatatcg tctgccaaag agataagatt gacggaaccg tttaataatt tgaccgcgcg
     4381 acgtaactga cgcagtaaat catccggcgt ttttactgcc gacaggcaag taaaacgccc
     4441 ctctgacatt accgccgcca gctgtgcggc aaaaggctgc cgttcatcaa tggctttaac
     4501 attggcggca acggcggcga ccagtgccag tgcggtaaaa cgccactctt gtgcttcaat
     4561 tttccacaaa gtgtgggtct gcatcagcag agaacgaaac ccttctgaca agcaaacatc
     4621 attaaccgtt gtgctacgcc gcagactggc acgattgccg cgtttagcct gcaactcttc
     4681 gtgccattta cgcagcgtgg ctttgtgccc gtcttttaca atactcattc agcagcctcc
     4741 tgctttttct ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttt
     4801 ttgcgcgcgg tcatgacgcg ttttaaatca acgggctcat aaggattggt gaatacacgc
     4861 tcgtcaaaat cctgacgcgc gaataaccaa atctccttat tccatttgct gagtaatcca
     4921 tccgcatcct gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg
     4981 ttccagaagt cgatatccac aaaactgaaa tcaccccgtg caccttttgg atcggagaac
     5041 catgcttctt tcaatgcact ccgtaacaga cttagaatgc gtgaagccgt ttgcgcagcc
     5101 agccgcagtt tcggtatctg gccttctttt ttattgagca gcagcgggaa acggtgttcg
     5161 taccaacagc gggctttcat gttgtcgaaa tcatagccaa atccccatag gcctactttt
     5221 gcctgcttga gactgctggc attaaagagt ttcaccacca gagcgggaag ttccgtattg
     5281 ttttctgact tacccgtttc gataaggcct aaccagtcgc gccaaattaa accgcctggt
     5341 tgtggtttaa ctgagtaaaa ctcaccaccc tctttaagtg gtacacggta aggcgttaat
     5401 ggatgctgcc acatggcata attcgcaccg taatttttgg tcgtcatcag gctcagaagc
     5461 gcgtcgtcct gctcaccgca aatatcgcag ttgccgactg tcgtggtatt aaaatcaata
     5521 cgaatacgcc gcggcattcc ccagtacgcc tggagtttat tgacctgatc atcggttacc
     5581 accgcaccgg ccagttcgct ggtacgcgtc gggccaagcc aggggaaaac cagatcgtca
     5641 aattttttgg gtagcggtaa gtcggcttca tcctgcggca tcacgttgag ccacagtttg
     5701 cgccacaagg gggcttgttg attgccctga tactcctgca attcaatcag agtcgtcatc
     5761 ggcccaccgc cgcgtatacc ggtgcgatag cctttgccac ctgacggcgc atttaactgt
     5821 agggagaaca gagctaacgc agaacaatga gagcatacgt gttcagtcac gccacgctta
     5881 ataaagtggt ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg cagtagcgaa
     5941 gcgacctgaa ctttatcgcc catgagcacc tcgaaatcct gcataaatga aggtgaatct
     6001 gggccaaact ggaaagcgtg ttctaatgac agcaatgctt cccgtagctt ttcagcttcc
     6061 agcccgtctt cccagatatc atcccaacga cgataatctt ttggcgcgaa actgctttgt
     6121 agtaacccca gcaaaaactg ccatgccgcc ccctggagat ctgcccgcgg cgcagcgata
     6181 tcgacaacat tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta
     6241 aaacgaacgg gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg
     6301 taaaaaagac catccctgat cggttatcca tcattccatc tttttaggtg tatcaactat
     6361 tcagagagct ctatcgcagg catctcaacc aaccttgcca atcaatccct ccgtagccga
     6421 atacccacaa ctttcgttat cggtgactaa aatcacgttt gccatctctg gatcttgccg
     6481 ctgttcaatg caccactgcc tgaacgcttc cccttccagt aaagaaaact catcgcgatg
     6541 ttttttccac cagcttcgac gcactctgac aacactcatt tcccatgcgt gagcgccagt
     6601 ggtataaggc ttcaccacac cgtcaataca ggtagccagc cacagggaaa cagattcctc
     6661 agccagacgt gtcgacagtt tttcagggag gtaatcgttg atattggcgg catagccggg
     6721 cctgaagttg agtacaaact ttttagccat tgcacgatcg cagtaatatt tgcccacttg
     6781 ctcctgctcg gagcgagcaa acccttccgg catcaccacg tcctcaccgt agactgattc
     6841 aataagaagc cgggctgagt gtggcatttg aatagcgcct tgctgacgca gtacacgctg
     6901 cgtcagccag attcgtccat gatcgggata gatatatgca ctgttccgca tggcactgcc
     6961 aaaccattct tcatcaggtg aatcgtccca gacgggggcc agaatcagca attcaggagg
     7021 ggaacgctcg tcttttccat cacgctttaa ctgaccatta atatcgcgga tatgccgctg
     7081 taatcgcccc gctcgctgga tcagcaaatc aatgggggca aggtcggaaa tcatttcgtc
     7141 caggtcgcaa tcaacgctct gctccagtac ctgagtgcta ataagggctt ttccggcacg
     7201 ctgtgagcta ccttctttac caaagcgcgc cagcgttttc gtttcgattc gctggcgatc
     7261 gctaaaagca aagcggctat gaaagagtga aaggctggaa gtgggaatga cgccgcgggc
     7321 aagcagctga cgatgaacct taatagcgtc atcgacagaa ttccggatcc aggcgatgca
     7381 ttttccctga cttaccgccg actcgatacg cgcaatactc tcttgttcac tatgaagcca
     7441 acccacgctg acgctacgct caacgtcttt gcgcgtcgct acccggtgtg agttcacatc
     7501 ggatttcgtg acatgcgtca gccaggggta atcatccttt tccaggaacg gagcttcttg
     7561 ctggccctct gtaccccgcg caaaggcagc gacgagtttg tcgcgctgct gttgagataa
     7621 cgtggcagaa agcaaaataa cgctgtttcc gccacgcgcc tgccgctcga tcaacccttc
     7681 aagaatgcac gacatgtaag cgtcacaggc gtggatctca tcagccagca ggattttgtt
     7741 actcagcccc agaagccgca gattattatg tttgaacggc atcaccgcca tcatcgcctg
     7801 atccagcgtg ccgacgccaa tttcagccag caacgccttc ttgttactgt cggcaaacca
     7861 ggccgcacat ccctgactga acgtttgttc atccggttct tctgacccga ctaaatcacc
     7921 agaccagagt gattcattaa agcggtccat taatgtgcgg gcactatgtg ccagcaccag
     7981 gctggggcgg gattctggcg aatagaaagc aagccaggtt ttgaccagcc gatcgtacat
     8041 ggcattggcc gttgccattg ttggcagacc aaaaaacagc ccctgagctt tccccgcagc
     8101 tatcaacctg tgcgccagga taagcgccgc ttctgtttta cctgcgccag tcacatcttc
     8161 cagaataaat aactgcggtc cttgctggct gatatccaga tccagcacct tttgctgtaa
     8221 cggtgtcgga ttctcaataa aaggaaacag cgtattaatt ccgttgaaag gcgcgacttt
     8281 tgcttttaga ggaaagacgg ttaaggcgtt ttgagcctga attaaagttt tctgccagta
     8341 acctttaata tccattgggt gtgcgacgcg tggaaaaaat cgcgttgacg aacccgtcca
     8401 gtctgcgagc acgacggttg cagagatata ccaggaaagg tgttttataa gttcaatgcc
     8461 ctcatcatca tcccagaatg cggggatctc tatgagcgga aacagtggtt tgatttcaag
     8521 gagaaaatct cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg
     8581 atcagggggc tgaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct
     8641 aagtttgcgc ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg
     8701 attccacaac cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc
     8761 ttcctgaatc ttgagttcag ggggcaggta aagttgttga aaagagcggg caaattttcc
     8821 aatatcgtgc cagcacagca accaggcgaa aaattgagcc gcctgttcct tgtcagaaat
     8881 ccctaattga cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc
     8941 catcgcggct acatccagcg aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt
     9001 ctcgccgcgt cgggtttttc cccagaaatc aaaaaaagtc acaatatttt tatccttcag
     9061 taaacttaaa ggatatttac gcatatttta aaaattatct gtgatatata tcagccaata
     9121 aaacatttac attatgaata aattaatgat tttcatttga aattcataat aataaataga
     9181 gactatatat tcacataaaa aatataaatt ttaaaaaagg gtgcaggaga ccaaaccatc
     9241 acaattcagt acgcggggtt ttacccccgc gtggtggcta cttcttcgat tcgtaagcga
     9301 gaactgcctt aaactccata ccacgttctc tgatactcaa ctcacacaac gagtcaccta
     9361 ccatcagcgt gaatcccagt actgtacaac acagtacgat gattgccaca agggcatatt
     9421 ttgtcagcat gtcttgcctc cctgcaaaga agagactaac attcgaattg tttgggttcg
     9481 aacgtaagcc tcaggttgat agaaatatcg cctggggctt ttgtccatct ggaacctcgc
     9541 gaatgcttaa cgccagacag cctcaagcac ccgacgccat tctatacctg ataattcttc
     9601 cagcggcgtt cacgtttaaa atcaggaata ctgctcgcaa aataaaaacg cccccatcat
     9661 ttctgacaga ggcgtttaat ttatccggca atatttaccc ttcgtgcaat ccacattccc
     9721 tttttaagcc aaagaaacgt gtttcttctt ccgacatgcc aggttcccat ttacgggttg
     9781 tatgggtatc accgaccgat aaatatcctt catcccataa tgggtgatat ttcaggccat
     9841 gtttttgcag gtactgataa atagttcggt tatcccagtc gataatcggc agcactttaa
     9901 atacgccacg ctgaattgcc agcaccggta aattggcgcg gctgccggat tgttcgcggc
     9961 gcaggccagc aaaccacgtt tgcgcattca gttctttcag cgcccggttc atcggttcga
    10021 ctttgttgat gtcattgtac ttttcaatgc cttcaacgcc ctgttcccac agtttgccgt
    10081 agcgtgcttc ctgccaggcg gcgctttcgg tagcgcggta cactttcagg ttgagcttga
    10141 gtttgtccgt taactcgtca ataaagcggt aggtttccgg gaacagatag ccggtgtcgg
    10201 tgaggatcac cggaatatcc gggtgaatct gattcaccag atgcaggctc accgccgcct
    10261 gaatgccaaa gctggaagaa agtacatatt caccgggcag attatccagc gcccaggcaa
    10321 cgcggccttc agcgtccagt ttttccagtt cggcgttagt ttctgccagc gccagaatgc
    10381 gatctacctt cggcagttcg ttcagggcgt ttagatcgag tttggacata gattcctcac
    10441 tgtttgcctt gcctgatgcg acgctcacgc gtcttatcag gcctacaaga ccgggctgat
    10501 ggttaatccc acaaatcgcg cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc
    10561 gtaaagtcgc cgaagccttc acccgcttcg cgctctttcg cccagcgccc tatcagttca
    10621 tcaagcgacg ccaggatttc cggctcggtg atgttttctt tatacatccg tgggatacgt
    10681 gtcccaatgc ggttgccgcc aagatgcaga ttgtagcgac ccggtgcttt acccaccagg
    10741 cccacttccg ccagcatcgc gcgaccacaa ccgttcgggc agcctgttac acgcatcacg
    10801 atatgctcat cgctgacacc atgtttcgcc attaaattat cgatgttgtc gataaaagac
    10861 ggcaggaaac gctctgcttc cgccatcgcc agcgggcaag tcgggaatga cacgcaggcc
    10921 atcgagtttt cacgctgcgg cgtgacggca ttcattaacc cgctctcttt ggcgatcttc
    10981 tcgatcttcg ctttctcgct ttccggtaca ccggcgatga tcagattctg gttcgccgta
    11041 atgcggaaat cgcctttgtg gatcttcgcg atctccagca ggccggtttt cagcggacgc
    11101 cccggataat caaggatgcg accattttcg ataaacagcg tcaggtgcca gttatcatca
    11161 atgcccttaa cccagccaat acgatcgcct cgtccggtga actcatatgg acggatcggt
    11221 tcaaatttga tccccgcgcg acgctccact tccgctttaa acgtctcaac ccccacgcgc
    11281 tccagcgtgt atttggtttt ggcattttta cgatcggttc ggttacccca gtcacgctga
    11341 gttgtcacga cggcttcggc caccgccagc gtatgctcca gcggcagata gccaaactcg
    11401 ctcgccgtgc gggcgtaggt tttcttgttg ccgtgttcga tagaaagccc accgcccacc
    11461 agcaggttga agcctaccag cttgccgttt tcggcgattg ccacgaagtt catgtcgttg
    11521 gcatgcagat cgatatcgtt ctgcggcggg atcactaccg tggttttgaa cttacgcggc
    11581 aggtaggtct gaccaaggat cggttcttca tcagtagtgg cgactttttc ctggtcgagc
    11641 cagatctccg catacgcgcg ggtacgaggc aacagatgct cagaaatctt cttcgcccac
    11701 tcgtacgctt ccgcgtgcag ctgcgactcg taagggttcg aggtgcagag tacgttacgg
    11761 ttcatgtcgt tagctgtcgc cagcgcatca agaccgaccg agtgcagcat ctggtgcacc
    11821 ggtttgacgt tctttttcag aatgccgtgg aactgaaacg tctggcggtt ggtcaggcga
    11881 atgctgccat agatggtgtt ttcaccggca aatttatcga tcgcttgcca ctgtttggtg
    11941 gtgatcacgc cacccggcag acgacagcgc aacagcatcg catggcgcgg ctccagcttc
    12001 tgttcagcac gttcggcgcg gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg
    12061 atcagcagga agttatcgcc cttaaagccg ccggtcagac cgtcgtttaa atcttccgca
    12121 atggtgccgc gcaggtagtt gctttcaagc ttcatgcgct cggcgtctgt cagtttacct
    12181 tcgaccacta aaggccctgg atgtttttcg ctcattagta gacatctcgc tgataacggc
    12241 gctctacgcg cagctcactt aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt
    12301 cggcaatcac ttccagaagt gcctgctcaa cgtctttcgc catgcgatta gcgtcaccgc
    12361 agacataaat atgggcaccg tcattgatcc agcgccacag ctccgcgccc tgttcgcgca
    12421 gtttgtcttg tacgtaaact ttttcttttt ggtcgcgcga ccaggcgaga tcgatccgcg
    12481 tcagcacacc ctctttgacg tagcgctgcc actccacctg gtagaggaaa tcttccgtaa
    12541 agtgcgggtt gccaaagaac agccagtttt tacccggtgc ttcgtcggca gcacgttgct
    12601 gcataaaggc gcggaacggc gcgatgccgg tgcctgggcc aatcataatc accggggttt
    12661 ccgggttagc gggcaggcgg aagttatcgt tatgttcgat aaatacgcgg agttcgccct
    12721 cttcttccac gcggtccgcg aggaagctgg aggcaccacc ggcacgggcg cggccttcca
    12781 cgtcgtaacg caccacacca acggtgacgt gtacttcgtt ctcgacttcc gcctgcgagg
    12841 aggcgatgga atacagacgc ggcgtcagcg ggcgcagcag attaattagc gcttcggcat
    12901 caagctgtgc cggggagaaa cgcaccatgt caacaatcgg cgtcgtcgcg gcgtaatgct
    12961 gtaacttcgc tttatcgccc accagcggca gcagtgtttc actgcgggta agcgtggcgt
    13021 aattctcaac aatgttggcg gtgttgacgg tcagttcgaa gtgccactgt agcgcttcgt
    13081 tcagaggcaa cgttttgccc tcgacggtga caggttcatc gcctgtcagc cacagcagtt
    13141 cgacaagttc tttcaccagt gccggatcgt tctgatacca gacgcccagc gcgtcacccg
    13201 gctggtaacg caggcccgag tcacctaagt caatttcgat atggcgaacg tctttttcag
    13261 agttacgccc ggtaattttc tggttaacag agaggctagc caccagtggc gcgtctttgc
    13321 tgtacgggct ggtgtggatt tcatttaccg cgccagtagc gacggattgc gaaggtgccg
    13381 cgacaggcgc acgcgattta agcgcatcaa ccacgcgggc gcgccactcg ctggcagcag
    13441 cctggtattc aacatcggca tcgacacggt cgagcaggcg ttcaccgccc agttccgcca
    13501 gcttgctgtc gaaatctttc ccggactggc agaaaaattc ataagagcta tcgccgaggc
    13561 taaacacggc aaacgcggtg ttttccagct ttggcgcttt tttggagaac aggaacttat
    13621 gcagcgcgac ggcttcttcc ggcggttccc cttccccttg cgttgacgtc accacgataa
    13681 gcagtttttc gctggcgatt tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt
    13741 tcagttttgc cgtcaataaa tcatcgcgta atgcttcagc aacccggcgc gcattgccgg
    13801 tttgcgaagc agagataata gttatacccg gcatttctgc ggctggcgct ggcgtcgctg
    13861 caagcgcagc aggctgctga ttgagcacgc cccagaaata gccagaaacc caggcaagct
    13921 gggtgggagt taaatcggtc gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg
    13981 gaagcaacgc ggaaggtggg acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag
    14041 ctgtttctgc gccctgtcag cgcccataaa acagaagaga aggtaaggtt aacggggcaa
    14101 atggtgtgga ttaaagacgg gatagcgata actaataacc aaatcgacta acctgtttta
    14161 gcaatagtct ttaacaacaa aatagattaa ccaacccaat gaaaaacaaa tgaatttagc
    14221 caatcattaa gataaatcag cgattttgcg caacaaagtc gttttagata atgcgaaaaa
    14281 acagcctttc cggtactcta cggcggtttt atcgtcctgt agagaaatta tgatgtccac
    14341 cacgttattt aaagatttca ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg
    14401 gcataaatgt ggtcgcctgc acgggcattc ctttatggtg cgactggaaa ttaccgggga
    14461 agtcgatccg catacgggct ggattatcga tttcgctgaa ctaaaagcgg cgtttaaacc
    14521 aacctacgag cgcctcgatc accattatct caatgatatt ccaggtctgg aaaacccaac
    14581 cagcgaggtt ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc cgctgttaag
    14641 tgcggtgatg gtaaaagaaa cctgtaccgc aggttgtatc tatcgcggcg aatgataaga
    14701 gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta gggtgttgtg ttctggatat
    14761 ctggggcatg acatggaaga cgactgcgac attattatta ttggtgccgg tattgcaggc
    14821 accgcttgcg cgttacgcat ggatatatcg ttcgatactg gaaagcgtgg ctctgatgct
    14881 gaagaacaat tacgacaata tgcgtaatga aatgaatcac tttgcaaagc atgtgatcat
    14941 tactggcggc ggttcgaaca gcgatctgtt tatgcagatt tttgccgaca tgttcaacct
    15001 tccggcacga cgtaacgcca ttaacggttg tgcaagtctg ggggcagcga ttaataaggc
    15061 ggtaggtctg ggactatacc cggattacgc aacggctgtc gacaaaatgg ttcgcgtgaa
    15121 agatatcttt ataccgattg agagcaatgc caaacgctac gacgcgatga ataaaggcat
    15181 tttcaaagac ctaaccaaac ataatgatgt aatactgaaa aaatcgtatg aagtgatgca
    15241 tggggaattg gggaatgtgg attcgatcca gagctggtcg aatgcgtaag tgaggaaggc
    15301 cgggcgggaa actgcccggc ctgaacatac ctgaatggtt atccccgctg acgcggggaa
    15361 cataagttcg acaacttaaa aaatgataac aagtccggtt tatccccgct gatgcaggga
    15421 acataataaa aactttaact agttcggttt atccctgttt ttacaaggaa tattaactac
    15481 actcgatgtc tttagaataa aagacaatac gaatcgcact ttatcaattt tcacttaaag
    15541 tatgaactgg atatctttat tcataagaac tattcatcaa cagcgtatag aaacggtaat
    15601 gtaaaaccac tcattagcct caaaacaaag acaaattttc agatttatta cttaatcaat
    15661 gaataacaag cttaaaaaac aaactattaa cctaaagaaa aaattaatag atattaattc
    15721 aacattaaaa aaataataga ttaaaatttc ttaacgattt aagaatcata caaataacac
    15781 tttgattaaa tttaattttt tgtatcgata attgtgaact tctctggcat ggagaactat
    15841 tttgaacatg aggggttacg tggatatgtt gcttattaca agtactgcta atataaaaac
    15901 ttgagaaaga gataacgggt tatatggtgg tttatccccg ctggcgcggg gaactctttt
    15961 gtgccctggc gttttgctgg ctctcgcgcg gtttatcccc gctggcgcgg ggaacacttt
    16021 gccgctgtca gcattgctgg cgcgtaatac ggtttatccc cgctggcgcg gggaacacaa
    16081 aattcatatt gataaacacc gcgtttgtat cggtttatcc ccgctggcgc ggggaacact
    16141 ttactacaat aggtggacac gctacggcgc acggtttatc cccgctggcg cggggaacac
    16201 aaatatgggg agcgccgtta atgaaatcaa aacggtttat ccccgctggc gcggggaaca
    16261 ccgccgcgga ctcacgagca ggccatcaca gaccggttta tccccgctgg cgcggggaac
    16321 accagttgtc tgatgtgtcg ctggcctggc ggcacggttt atccccgctg gcgcggggaa
    16381 cacggaccaa atacccgtcc ctcaatccac gccgtcggtt tatccccgct ggcgcgggga
    16441 acacaattga gacgttccac gcaattaaac aagccgcggt ttatccccgc tggcgcgggg
    16501 aacaccggtc gtcaggtact aatctaaagg ctgttttcgg tttatccccg ctggcgcggg
    16561 gaacacgtgg cgtccgtcgt cgtttaaagc gtcaatgccg gtttatcccc gctggcgcgg
    16621 ggaacactct aaacataacc tattattaat taatgatttt ttaagccagt cacaatctac
    16681 caactttata gtatcacaca aacaacacat ccattatgtt aaagagtact taatccattg
    16741 attaaaaagg taaatattta aaataactct atacaaacta aaatctacca aactttaccg
    16801 caataatttt cactccagcg aaaaattaat gccacagaat ttgtagagaa tgctaatgat
    16861 tagccctgga tgggtaaaat ggaggagttt tcagaggcga caaacaatat aaatgagtag
    16921 aagtctttac aggttccacc catttataac gcttataaat gtttaatcag gcaatattta
    16981 gatatttatg tgtttgcatc gacaaacgcc aattacgcgc aatgcaggtt tcaatgcaca
    17041 aacgtgtggc atcatccttt tggctaatcg gctgcagtgc aatgactcgc ggtttatcat
    17101 cggtcagcgt tgccaatagt tcatctagtg cttcaatatc gcgtacgcgc cccactggat
    17161 gcttgatttc gttggctcgc tccagtgcct gtgacaacac gtcatagccg ccgcgcatgt
    17221 tcagctttgg cgatacggta acccaggtat tcggtgtgca gcgtacctca tgagtaccac
    17281 tggtttcgat ctggcagcta aaaccgttct tttcgagcag atcagtcagt ggtagcaaat
    17341 catgaatgca aggctcacca cccgtaatca ccacatgccg cgcggtgtat ccctggcgac
    17401 caatgacagc cagcaaatct tcactgctcg ccgaccccca cttatcactc tctttggtct
    17461 tcgccagaat gctgaaaagg gagacttccc gatcctcaag cttttcccag gtgtgtttgg
    17521 tgtcgcacca ggcacagcca accgggcatc cctgtaaacg aataaaaatg gcgggaacgc
    17581 cggtaaagta accctcacct tgcagggtct ggaacatctc gttaatcggg tactgcatag
    17641 cattctctgt gaagtggata attgttaatt attgcagatc ctgccgcaac aatcatgtct
    17701 tattaacatt ctgttacagg caggtttaag gcaaaaagtg cattagatca taaagaataa
    17761 aatgatgcga cgtattatgt tttaaggaat taatgaaaaa ggatttaaac atgctcagga
    17821 cattcatcgt aattatattt attttcaatt tatcaggctg tggatataac gatattcaaa
    17881 cctatgatga gcaggttaac gcctcctggt ctgaggtatt aaatcaatat caacgtcgcg
    17941 ccgatcttat tcctaatctt gtcgccagta ttaaaggtta ttccaaccat gaaaaagacg
    18001 ttttggaagc cgtaacattg gctcgtagcc aggctaatcg cgccagcagc gatcttcagc
    18061 aaactccggg aaatgaacaa aaattacaag cctggcaaca ggctcaagcg caggtgactc
    18121 gcactctcgg tcagttaacg ataattagtg agcgttaccc tgagttaaag gctcaagagc
    18181 tctatcaaaa cttaatggta cagctcgaag gaagtgaaaa tcgaattgcc gttgcgcgag
    18241 gaagatatat taaagccatt gaacaatata atgttactat ccgcaaattc cccgctgtat
    18301 taaccgcaaa ggtcatggat tacacaccga agaaaaatta tttgccggat gatgtcgccg
    18361 ctgtgagtaa agcaccaaca atagatttta gccagaatgc tagtgctcat taggcgagtt
    18421 aattatatga aaaatttaat cttacttctt gttctgatct gttcatctgc gtttgcagtg
    18481 caagttccag tacctgaatt cgctaagtat ataaatgatt taacagggac attaaccagg
    18541 gaggaagtaa gtacgctgac cagtcagatt aaaaccctga cgcaaaaaag ccacgcacaa
    18601 ctgatcgtgt tagtagtaga aacaacaggc gatgaaacca tcgagcaata tgcaacacga
    18661 gtattcgagc gttggcagcc cggtcataag aatcttgatg acggtatttt actggtagtt
    18721 gcctggcaag atcatacagt acatattgaa attggctacg gacttgaggg tattctgacg
    18781 gatgcgcagt ccgggcaaat tatacgcaac agtattattc ctgcgtttaa aaagggcgat
    18841 cttgctgtcg gcttgcaaca aggtattggt gatattgaaa cccgattatc agaaaatgat
    18901 ttagtcaata atacatcgcc cgatcatcct ttacccttta gcggttggtg ggctctgtta
    18961 gtctgggcga tagtactgac gtttatttct gccagaggat atgtcaaaac gctgggagtg
    19021 atttgctttg ctgccataat actggccttt gtacttccca tcgctggata tagcgggagc
    19081 tggggagttc ttgccacatt attatgtttc gccactccat tcctggtcgt agctatcgct
    19141 ttcacgcctt tcggcagaaa ggtgcgtgat tcaatgcgta gcgccaaccg accgtcacgt
    19201 tacgaaagaa gcaactcggc ctcccgacat tcttcatcat cgtcatcttc ctcttcatcc
    19261 tctttcaaca atgacaattt cagcggtggt ggcggttcat ccggcggtgg cggcgcgtca
    19321 ggaagatggt aagacttaaa aacagattac agggaaggta aggaaatatg agtaagattt
    19381 tagcgaatca tccacgatta agtaaatttg tatttggctt attcggtgct gctggaatga
    19441 tttggcttaa taaagccttc cagggcgatg aattatttca actgataaca tcactgcttg
    19501 ctttagatac gtttgtgtac ttttgcatct acagtctgat taacccaata cctgaggcaa
    19561 atgattttct ttgggattat aaagtccatc aacgaaaata ttcatccaat atttctcata
    19621 atgattttgt cagcaatgta aggaatagca gaaaaattgt tcggatactt atcctggtgg
    19681 gaataacatt tattaatgtt atcgctctgc ttactaaact caaaatgtga agataaaaaa
    19741 acccgcttca agaagcgggt tttttattaa gacgttaagt cagtcttatg cctggccttt
    19801 gatctcttta cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag
    19861 ctggttgtat ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc
    19921 agcagtacca acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga
    19981 gataactgca gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag
    20041 agaaccgatc tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc
    20101 tttcaggatc ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc
    20161 cagaactttg gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat
    20221 agaaacgatc gggtactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt
    20281 gaacgctttg ttgccttcgc cagccagaac gtatttacca tctttgtaga attcagaagc
    20341 tgcgcagtcc atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc
    20401 ttcagcgata acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc
    20461 gtcaccaaca gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac
    20521 ttcagaaccc atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa
    20581 ttcctggata tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg
    20641 aaccggcata gagtatttgc ccggagtacc gttcagttca gcgatgtgct cgtacagcgg
    20701 cataccttta gcagctgcag cagctttggc gttagccaga gatacagcca ggattgcgtt
    20761 cgcgccgaat ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc
    20821 agcctgatct ttagcatctt tgccaatcag cgcctgagcg atcgggccgt ttaccgcagc
    20881 aacagctttg gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag
    20941 agcttcacgg gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc
    21001 ctccagatgt acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc
    21061 gatgattttt acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc
    21121 cagacaaaca acgcgtaccc agggtacgcg ttgccgttct aactttttta cttacttcgc
    21181 ctgacgcttc tggaactcgc tggcggcttt tacaaagcca gcaaacagcg ggtgaccatc
    21241 acgtggagta gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg
    21301 aacttcgatg atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc
    21361 atcttcaatc tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac
    21421 aattgtcggc gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg
    21481 tgcgccgaga cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc
    21541 gttttcatcg cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa
    21601 ttccgtagag ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac
    21661 ctgcataccc aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt
    21721 cgtaatcatg ccttctacgc cacgatagcc aaaaccgcca ggtactagga ttgcgtccag
    21781 acctttaagg atttcaacgc cgcgcgtttc aacatcttgt gaatcgatta gtttgatgtt
    21841 gacgctgaca cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc
    21901 atccggcagt tcaatgtact tgccgaccat accgatggtg acttcactta ccgggttcgc
    21961 ttcttcgaag ataacctgtt cccattcgga cagattcgct tccgggcagt ttaagctgaa
    22021 tcgtttacaa atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat
    22081 ggaatcgacg tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat
    22141 cttcgcacgt tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg
    22201 gataccgatg gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc
    22261 agacgctgcc atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat
    22321 ttcaacagcc atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt
    22381 accgccgatt tctaccagta cgacgtcatg accttcgcca ccttccagca cgcgctcttt
    22441 gattgcgtta gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg
    22501 ttctttacgc agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat
    22561 tttggtacga atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc
    22621 agtaacgaac acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta
    22681 cggatccagt ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga
    22741 ggctgcggca atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt
    22801 cgttgtcatg ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga
    22861 aagcagtata ccctaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag
    22921 cgcactattg aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa
    22981 atcaagcgct tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag
    23041 agatcaattt cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca
    23101 cctgtcattt ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg
    23161 aatttttcgt tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga
    23221 ttaaccgtgg caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca
    23281 gcctgccgcg cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg
    23341 ccaagcgtcg tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc
    23401 attaaagccg gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg
    23461 cgctcttcgg ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa
    23521 ctatcagcaa aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata
    23581 tcattaaagt caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat
    23641 agcagatcgc ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc
    23701 acttcgtagg tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc
    23761 cacgggcagc cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt
    23821 tgattcattg aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat
    23881 agaagtgagt tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa
    23941 ttcctctggg gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt
    24001 tacctgatca gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg
    24061 atatcctttt gattgcgttg tataaggaac acacagacac atacctgttt tgttgttgta
    24121 catgaaagga ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg
    24181 gtcaaaatca acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag
    24241 acttccttat ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg
    24301 tcgttgacca gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat
    24361 ttgccatcca ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt
    24421 aacgtagccg ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc
    24481 ataacccttt cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta
    24541 gatacgagca aatttcggcc taactcccgt gcaaccgacg cgcgtcgata acatccggca
    24601 cctggttgag tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca
    24661 tgtcgatggt cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca
    24721 ccttctcgtt ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca
    24781 ccacgcggac caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa
    24841 tgcgttctgg cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg
    24901 aaataccgcg cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc
    24961 agcgcgcgat gtggtgcatc aggttaccga caccttcaac caccacgcga ccgttatctt
    25021 tactgcggtt ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct
    25081 gctcttcggc actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac
    25141 ggatatcccc gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg
    25201 gcagcagatg tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa
    25261 ggatttgccg cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa
    25321 ttttcgaacg cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc
    25381 tggggttcgg ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg
    25441 tgaacggcac aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt
    25501 ggatgtggta agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt
    25561 tcggcgtaaa gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt
    25621 cgccggaatc agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt
    25681 cttcatgtcc cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag
    25741 caacacccaa ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt
    25801 tgccacctgg ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag
    25861 cgacataatc gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg
    25921 cggcatagca atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct
    25981 catcaaaggc gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac
    26041 gaccatacac ttcagcttta acgccttcag ctttcatctc agcgcgcaga tgaccgacaa
    26101 actcttcgat atagtgttcg cggtcgagac ggcgttcatg cagcagtttt gcgatgcgtt
    26161 tgtattcggt cgggtgcaga taacggaagc agtaatcttc cagctcccat ttcagttgcc
    26221 caatcccaag acggttcgcc aacggcgcgt agatattggt gcactctttt gccgccagta
    26281 cgcgttcatc ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt
    26341 tgatgaccac gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga
    26401 cctgttcgga ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat
    26461 cacgcacgcc gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt
    26521 cgctaactac gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt
    26581 ccatacttaa tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg
    26641 catccggatg cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac
    26701 acgacttctg gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag
    26761 ccttattgat atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct
    26821 gccgaaatcg gcaaatcgta actatttaac gcgcgagaaa agtaccatcg attccagatg
    26881 tcccgtgtgt gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa
    26941 taacgcttcg ctatcccgag ccagcgttgc aggattacag gatacataaa ctatacgaat
    27001 aggttccagt tttataattt gctgcataac acctgcggca cctgctcgcg cggggtccag
    27061 caacacttta tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt
    27121 ttcgtgataa aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc
    27181 caccagcgcc ggaacacctt ctacacccac cacactggca gcttgtgtcg ccaatggcag
    27241 tgtaaagttg cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag
    27301 ccattccaac gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc
    27361 gcgcgggcta aaagttaagc gcaacccgtt tgagtcatac cagggcatct caccagagac
    27421 ggtttcgagt atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa
    27481 gcgttccagt ttttcgcgat cagccgaact tagcggtgcg gtatggcgca aaatcatcag
    27541 cgtgccgctg gttgcctgta ccagttcaac atgaccaaga tggcgtatag cttgtaagct
    27601 gcccaggcat gccctgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca
    27661 ttgtttgacg tcgacaatgt cactggagcc cgctttgcga aagcccatct gaagttgctg
    27721 tgttttcggt aagtagttca aacttaaacg cgcgcgacgg cgatagcccc agggaacatc
    27781 ggcgatcact tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg
    27841 ctgctgtaaa tccacgctgg cgtgttgttg ctgacagcca ccgcatacgc caaaatgagg
    27901 gcagcgtggc gtttcgcgtt ccgggctatc gcttaaccgg cgcacgactt tagcgcgggc
    27961 gtactgtttt ttatcttcag taacagtaac ttccgcgttt tcctgtggca gtaatccagg
    28021 aataaataac gctttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt
    28081 gactgaaacg gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc
    28141 cattggcgag actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca
    28201 tgaccaacta cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta
    28261 ttggtttatt gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac
    28321 tggaagatgc cggtgccagc attattgagc cgcttgcagt ttctactgaa tatggcatga
    28381 gcctgcaaaa tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg
    28441 atattgttcg cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt
    28501 ttcatcttga tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca
    28561 ctgtcactcg tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact
    28621 cccccgacga atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg
    28681 cgcttgagct ggatcttaaa tcggttcgtt tgcagcaata taaagagatc tttatttcca
    28741 gcgtgatgat gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg
    28801 atgtaaccgg tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac
    28861 tcgacagccg ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca
    28921 actcgatggc aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg
    28981 cgacgtccga tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc
    29041 tggcgaaaaa gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt
    29101 cacacgagct gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa
    29161 cagaattaac accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt
    29221 tgctggcaat tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc
    29281 tggaaagtat tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac
    29341 attcttctca cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata
    29401 acgtgatcgg cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa
    29461 ttaaattcac cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata
    29521 ccaaagtgca gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc
    29581 aatcgcgctt attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg
    29641 gcaccggtct ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt
    29701 cgttccatag ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga
    29761 acccgaacat tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct
    29821 atgtcgaacc aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc
    29881 tggaagtggt ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt
    29941 tgttaggcat cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga
    30001 aagcggtatc gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg
    30061 cagaaaaact caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc
    30121 gcctgttgcc tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa
    30181 ccgatgaaag caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga
    30241 aacttatcgg cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc
    30301 atcaggcggt tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa
    30361 tgcctgacat ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac
    30421 aaacgccggt tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg
    30481 gcgcagggat gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt
    30541 tgttgcgcta caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg
    30601 aaattgtggt gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag
    30661 gaaaaaccga tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc
    30721 gcaacaaagt tgaggaacag ctggttggag aaaacccgga aggcctggtt gatttgattc
    30781 ataaactgca tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac
    30841 tgatcgaaca acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg
    30901 aactgttgga cgagatggat aatgtagcgc gcgaagccag caaaattctc gggtaacgga
    30961 cgtcttgtcg agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc
    31021 ctgtgtttat gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc
    31081 aatgaattaa aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc
    31141 atcagtctca agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg
    31201 cacgggcgct tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg
    31261 tcacgcgcgc caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata
    31321 tcgatttcta cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca
    31381 aacggttctt tggtcaggcg ctgattacct tcctgccaga tccagtgcgt atcaatagca
    31441 gtaattttac ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag
    31501 tggttgtttg agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt
    31561 accgaacctt gcatcgtcca gaaatgcgga tccgccagcg ggatatcaac ggattgcagg
    31621 gagagcgtat ggcccatctg ccgccagtcg gtggcgatca tattggttgc ggtcggcagg
    31681 ccagttgcgc gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca
    31741 cacggatctt ctgcataagc cagcgaccct ttcaggtatt taccaatttt aatcgcttcg
    31801 ttcagcgacc aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc
    31861 agtgccacaa tagactcggc ctcttcttcc ccggccagta caccgccttt cagtttgaaa
    31921 tcgttgaagc catatttttc atacgccgct tccgccaggc gcaccaccgc atccggcgtc
    31981 atcgcttctt catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac
    32041 ggcagcggtg tggctttgcg atcaccgacg aagaacagat aaccgagcat ttcgacttcg
    32101 ctacgctgtt gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc
    32161 agatccagca ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat
    32221 gtctgcaaac cgcgcccgcc ggcatcacga tcggcaaatg tattacgcac cagcgtcaga
    32281 acgtttttgt attcacccag cgttctaccc accaccagcg gaatcgcatc ttccagcgtt
    32341 ttacggattt tctcgccgcc gggaatttcg cctacgccgg tgtgaccgga attatctttg
    32401 ataatcacaa tattacgcgt gaagaacggt gcgtgtgcac cactcagatt catcagcata
    32461 ctgtcatgac ccgccaccgg gatgacctgc atttcagtaa caacaggcgt cgtaaattga
    32521 gaactcatat ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt
    32581 caaaggtcca gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag
    32641 gcagacgttt ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca
    32701 gaccgggcgc atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca
    32761 ggcgacaatc gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg
    32821 ccgccgcgcc cacatgggta aacatcgcca gagaaatatc gaaatggtta ttagaatggc
    32881 agccccaggt tagcccccag tcgtcgcaaa gctgtgccac gcggactgca ccggaaagcg
    32941 tccagaagtg cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatggccca
    33001 tttcgcgcca gttggtgacg atcatgttag tcgcgacggg caagccggtc gcccgtcgaa
    33061 actccgccat cacttcacga ccggagaaac cctgctcagc gccgcacgga tcttcggcat
    33121 aggtaagaac atcattcagc cctttgcaca aagaaatggc ttcatcaagc agccatgcac
    33181 cgttgggatc aacggtaatc cgcgcatccg ggaagcgttt cttcaatgca cgaacagtgt
    33241 cgatttcttg ctcgccaggt aacactccgc ccttaagttt gaaatcttta aagccatagc
    33301 gatcctgtga ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc
    33361 gcaactgata ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg
    33421 tccgatcacc gatataaaac agataaccga ggacggtaat agcatcgcgt tgcttgcctg
    33481 gccctaacag ttcgcagacc ggaacattca acgccttacc tagcaggtca agcaaggcgg
    33541 cttccagcgc cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg
    33601 tatcaaaatc ggccgcctga ttacctatat gcacctgctg aaccacttta ttcaggcgcg
    33661 caacttcctg gcccagcacc atcggaatag catcgacaag cgtctgataa atcacctctc
    33721 cgcccggcgc ttcaccaata ccggtatgcc cggcattatc ggtgagtacc acaatattgc
    33781 gggtgaaata tgcgctatgt gcaccaccaa tattaagcaa catgctatcg tgcccggcga
    33841 ccggaatgac tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc
    33901 gcgacaggtt tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc
    33961 gcgattaagg catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca
    34021 acgatataac caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac
    34081 aggccaccgg aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc
    34141 agcgccccga tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca
    34201 acgttgacgt agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt
    34261 gttttacgcg caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataatccca
    34321 cccagcacac cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc
    34381 agaatcgaca tcccacgcgc ctgcaccaga taaaccggga accaggtaat aaagaagtaa
    34441 gtcagggcgt tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc
    34501 agctgtttga tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga
    34561 tccatattga tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc
    34621 ggatgttgat ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga
    34681 ccgcccataa agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc
    34741 ggcgcgaaga tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc
    34801 ctttcctgcg ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct
    34861 tcagcaagcc cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta
    34921 aagatatcga cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag
    34981 tagacgcgtt ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca
    35041 taagcccatg agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct
    35101 ttggccattt ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg
    35161 acgataaaca acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc
    35221 gcagcctgac ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac
    35281 tctggactgc gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta
    35341 taggtcgttc gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg
    35401 ccatagcggc tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg
    35461 cattagccct ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag
    35521 ggttaaaacg aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc
    35581 agtaaaaatc cgcgctatga agcagttttt actggcattt gcctgaaaat attcgattca
    35641 gcaccgctaa aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact
    35701 gttcgaccca cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca
    35761 gcatttcgcc aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc
    35821 cattgcagaa atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg
    35881 gctggaagcc cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt
    35941 cgccctgccc ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt
    36001 cgcttaattc aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt
    36061 ctgcttcttc cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt
    36121 ccgccatacc gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc
    36181 actgaactca accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc
    36241 ctgcatccag gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg
    36301 cgtcaatgac aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc
    36361 gctgcgattc tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg
    36421 ggcaagatgt cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc
    36481 gtagcgcgtg gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg
    36541 ataatgcgtc actgctggct gcgagccttt atcttcgcgg gcaaatttat cagcgatttt
    36601 gtccagttct tccaccagtg gataatccag aactgcttct tccatcaacc agccgcgcac
    36661 aatcgcatgg taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc
    36721 ggcctcgctg gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc
    36781 ggtaaaaacg tgctggccaa tctgatcacg cacggtttgc atgaccacta ctttctcgtc
    36841 gcgatccagc cagctgcggt gaaccagcca gccggagggt ttatttaccg caaccagcca
    36901 ttcatcctga tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt
    36961 ttttccagct cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca
    37021 taatagggtg caacggcaaa agcgcccggt aacggctgtt tgttatctaa caaatcgtgc
    37081 attcgcggga tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac
    37141 ggttgggtac tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca
    37201 cgcagtaacg cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag
    37261 caacctcaat aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta
    37321 ttcacagcgc tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat
    37381 ccttgacaac ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg
    37441 ctcacaccac cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg
    37501 aatcatcctg accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc
    37561 atcctcttcg cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga
    37621 agtgtcatca tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg
    37681 tctttttcgc gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg
    37741 aaagttaaga attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt
    37801 ttaagtattc gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac
    37861 cagatggggt atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt
    37921 gaggctaaat gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga
    37981 ttacacaccg agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc
    38041 agttttagcc ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag
    38101 tcgtcttcac tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata
    38161 tccccggcaa actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa
    38221 gcgcgttcaa ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg
    38281 gcatcttcgg tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac
    38341 ggccagcttt tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg
    38401 gcttgtgcgg tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg
    38461 tttagtttat ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt
    38521 gcgggcctga ctcttggaaa atcaaccgat taccgggata cctatgacgc caacctactg
    38581 caaggcgttc cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct
    38641 tttcacggta cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg
    38701 ccgcaggtcg ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg
    38761 aagagtttta agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag
    38821 gtgcgccaga cgctggagcg cgacttaagc acttgcgccc agggtaaggt tagcgtggcg
    38881 ttatatcgtc ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacctgcatt
    38941 gatgaccagg atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc
    39001 accagcggtg aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc
    39061 ctgatcaccc atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt
    39121 gacagagaaa aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa
    39181 cagtgcgtgg aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattaagc
    39241 gtttacgcac gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc
    39301 gattttgtcc catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg
    39361 ctggattcac gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa
    39421 tgacgatatt cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc
    39481 atggatatgt tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc
    39541 tatcaactgt ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac
    39601 agcaaagaat tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc
    39661 ggcgtaaagc tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt
    39721 cgcgctttgc aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa
    39781 atccataata ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac
    39841 ctggtctttt ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg
    39901 gtggtctgct ggggcggtca ctcaattaac gaaaatgagt atttgtatgc ccgtcgcgtc
    39961 ggaaaccagc tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg
    40021 gaagcgccga tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt
    40081 tttattggta tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac
    40141 gaattgatca tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac
    40201 ggcatcatta tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga
    40261 attttaatga acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa
    40321 gagagcgccg actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac
    40381 gcgcgccgcc attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa
    40441 aaatcgatgc cgctggtgaa agaaaatcgc cgcgacactg gtgacgctta cagctttaac
    40501 tggtcaatgc gcattgcacc agatttgcaa atgccgtttg agccgtctca cgagaatatg
    40561 gctaatctga agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt
    40621 gcgttctccg gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag
    40681 tttggtcctt acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta
    40741 cagggttttg ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac
    40801 gaaatctgca cgtaatctcc cctcccccgg tgacgtttgt tgccgggttt tctcgttttt
    40861 ggtcacttac tcatcaactc atttcatttg ttatatgaat gtttcttacc accctcacgc
    40921 gacaaatatc atcacag
//
LOCUS       DAFATA010000028        40002 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFATA010000028 DAFATA010000000
VERSION     DAFATA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.028"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(303..1214)
                     /gene="hlyE"
                     /locus_tag="LGJ20_001941"
     CDS             complement(303..1214)
                     /gene="hlyE"
                     /locus_tag="LGJ20_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HBK2386009.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYIFLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            1587..2006
                     /locus_tag="LGJ20_001942"
     CDS             1587..2006
                     /locus_tag="LGJ20_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBK2386010.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            2006..3274
                     /gene="umuC"
                     /locus_tag="LGJ20_001943"
     CDS             2006..3274
                     /gene="umuC"
                     /locus_tag="LGJ20_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBK2386011.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(3320..3850)
                     /gene="dsbB"
                     /locus_tag="LGJ20_001944"
     CDS             complement(3320..3850)
                     /gene="dsbB"
                     /locus_tag="LGJ20_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBK2386012.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(3996..5537)
                     /gene="nhaB"
                     /locus_tag="LGJ20_001945"
     CDS             complement(3996..5537)
                     /gene="nhaB"
                     /locus_tag="LGJ20_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBK2386013.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            5759..6478
                     /gene="fadR"
                     /locus_tag="LGJ20_001946"
     CDS             5759..6478
                     /gene="fadR"
                     /locus_tag="LGJ20_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBK2386014.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(6530..8062)
                     /locus_tag="LGJ20_001947"
     CDS             complement(6530..8062)
                     /locus_tag="LGJ20_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBK2386015.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            8392..9690
                     /gene="dadA"
                     /locus_tag="LGJ20_001948"
     CDS             8392..9690
                     /gene="dadA"
                     /locus_tag="LGJ20_001948"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBK2386016.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            9700..10770
                     /gene="dadX"
                     /locus_tag="LGJ20_001949"
     CDS             9700..10770
                     /gene="dadX"
                     /locus_tag="LGJ20_001949"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBK2386017.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQEAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(11156..12892)
                     /locus_tag="LGJ20_001950"
     CDS             complement(11156..12892)
                     /locus_tag="LGJ20_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBK2386018.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12987..13901)
                     /gene="ldcA"
                     /locus_tag="LGJ20_001951"
     CDS             complement(12987..13901)
                     /gene="ldcA"
                     /locus_tag="LGJ20_001951"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBK2386019.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            14001..14612
                     /gene="emtA"
                     /locus_tag="LGJ20_001952"
     CDS             14001..14612
                     /gene="emtA"
                     /locus_tag="LGJ20_001952"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBK2386020.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(14614..15348)
                     /locus_tag="LGJ20_001953"
     CDS             complement(14614..15348)
                     /locus_tag="LGJ20_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBK2386021.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            15549..15803
                     /locus_tag="LGJ20_001954"
     CDS             15549..15803
                     /locus_tag="LGJ20_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK2386022.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            15981..16421
                     /locus_tag="LGJ20_001955"
     CDS             15981..16421
                     /locus_tag="LGJ20_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386023.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(16500..18197)
                     /gene="treA"
                     /locus_tag="LGJ20_001956"
     CDS             complement(16500..18197)
                     /gene="treA"
                     /locus_tag="LGJ20_001956"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBK2386024.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(18517..19935)
                     /gene="dhaM"
                     /locus_tag="LGJ20_001957"
     CDS             complement(18517..19935)
                     /gene="dhaM"
                     /locus_tag="LGJ20_001957"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBK2386025.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(19946..20578)
                     /gene="dhaL"
                     /locus_tag="LGJ20_001958"
     CDS             complement(19946..20578)
                     /gene="dhaL"
                     /locus_tag="LGJ20_001958"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBK2386026.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDDVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(20589..21659)
                     /gene="dhaK"
                     /locus_tag="LGJ20_001959"
     CDS             complement(20589..21659)
                     /gene="dhaK"
                     /locus_tag="LGJ20_001959"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK2386027.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            21887..23806
                     /gene="dhaR"
                     /locus_tag="LGJ20_001960"
     CDS             21887..23806
                     /gene="dhaR"
                     /locus_tag="LGJ20_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBK2386028.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(23906..26791)
                     /locus_tag="LGJ20_001961"
     CDS             complement(23906..26791)
                     /locus_tag="LGJ20_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HBK2386029.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFDAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHNDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(27632..28723)
                     /gene="ychF"
                     /locus_tag="LGJ20_001962"
     CDS             complement(27632..28723)
                     /gene="ychF"
                     /locus_tag="LGJ20_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK2386030.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(28840..29424)
                     /gene="pth"
                     /locus_tag="LGJ20_001963"
     CDS             complement(28840..29424)
                     /gene="pth"
                     /locus_tag="LGJ20_001963"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK2386031.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            29702..29980
                     /gene="ychH"
                     /locus_tag="LGJ20_001964"
     CDS             29702..29980
                     /gene="ychH"
                     /locus_tag="LGJ20_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBK2386032.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(30035..31714)
                     /gene="dauA"
                     /locus_tag="LGJ20_001965"
     CDS             complement(30035..31714)
                     /gene="dauA"
                     /locus_tag="LGJ20_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBK2386033.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(31839..32786)
                     /gene="prs"
                     /locus_tag="LGJ20_001966"
     CDS             complement(31839..32786)
                     /gene="prs"
                     /locus_tag="LGJ20_001966"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK2386034.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(32937..33788)
                     /gene="ispE"
                     /locus_tag="LGJ20_001967"
     CDS             complement(32937..33788)
                     /gene="ispE"
                     /locus_tag="LGJ20_001967"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK2386035.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(33788..34411)
                     /gene="lolB"
                     /locus_tag="LGJ20_001968"
     CDS             complement(33788..34411)
                     /gene="lolB"
                     /locus_tag="LGJ20_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBK2386036.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            34625..35881
                     /gene="hemA"
                     /locus_tag="LGJ20_001969"
     CDS             34625..35881
                     /gene="hemA"
                     /locus_tag="LGJ20_001969"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBK2386037.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            35923..37005
                     /gene="prfA"
                     /locus_tag="LGJ20_001970"
     CDS             35923..37005
                     /gene="prfA"
                     /locus_tag="LGJ20_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK2386038.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            37005..37838
                     /gene="prmC"
                     /locus_tag="LGJ20_001971"
     CDS             37005..37838
                     /gene="prmC"
                     /locus_tag="LGJ20_001971"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK2386039.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            37835..38227
                     /locus_tag="LGJ20_001972"
     CDS             37835..38227
                     /locus_tag="LGJ20_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBK2386040.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            38231..39040
                     /locus_tag="LGJ20_001973"
     CDS             38231..39040
                     /locus_tag="LGJ20_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBK2386041.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            39076..39930
                     /gene="kdsA"
                     /locus_tag="LGJ20_001974"
     CDS             39076..39930
                     /gene="kdsA"
                     /locus_tag="LGJ20_001974"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBK2386042.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         9976 a         9881 c        10212 g         9933 t
ORIGIN      
        1 ggtgaacgta tctctgtgcg tcatatcgca gtgaaagaat cagaagtgat tgactggcag
       61 gatcatatct taaataaacc tgactgggcg cagtgataaa tttaatagat gttgatgggg
      121 gctttcttaa tatacgtctg gatgctcgcc atttcgtagc cctgtttttg aaagcgctct
      181 gtcataatga gagttacccg ataccttatt atcaacaggt aactctcaat ctttaaaaaa
      241 cgtctgtgtt tttaaatgtg ataaacctta taccttgagt acatggagag aataatcgct
      301 tatcagactt caggtacctc aaagagtgtc tttttaccgt gtcttttctg atactcatta
      361 caggtgttaa tcattttttt ggccgcttct tttagcaaag aaagcattaa atcatcataa
      421 tcaacataga atctggttgt ttcagtttcc gtttttatct ccccgatggc ggctatttcg
      481 gtggttaatt tcaatttggc ggcatcgata tctttattcg cttgtttaac cgtgttagac
      541 agggtggtaa agaaactctg cacagatttt aacttgttct tcaattctgg gatcagtttc
      601 ccttcaacta cgcccgcagc aatagaatag gaaatgatta atccaaatgg accggcgacg
      661 acaccggctg cggcaccggc atacgcttcc ttcctgattt tatctacctg tgactggaaa
      721 tagctgcttt tttctgaaaa atcattggtt aactggctat ctaacgccag cagtttcccg
      781 gaagcgttgt tgaaactttg tgagcttacc agcagggatt tttgcgcttc attcagcttc
      841 gtgatgccgt catccagtac cttaatgaga atgtctttct gggcggatgc tttcttctca
      901 ttgtactcat caaatagaaa aatatacgct gcgagcaatt gcgtcgcaac accacaccat
      961 tcatacactg tttgggttgc ttcaaaatac ttatcctggc tatccataag taaggtttta
     1021 atatcgccga ctaaaacgga ggctgcctgt gaatactcct gtttaaagcg acttaactct
     1081 tttatggttt catcaaaggt ctgccagggg atgacctgat cgagatattt attataaaga
     1141 tctaatgctc catctgcagt ttcgattgcg tttttaacta cttctaccgt tttatctgca
     1201 acgatttcag tcataatcat tcgcctcttt aaatatataa attgtaatga aactcctgtt
     1261 ttacaactat taataaattt tacttcatct aattcatagt tagccgggcg ggatgcgtca
     1321 atgtctttat ttctattaat atgataaata tcaaacaatg tttaatgtca ttatggcgaa
     1381 tgcttctatt ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc
     1441 gccagaaggc cacgtgagca caggataaga gaacgaaaaa tcagcagtct atgcagcgac
     1501 aaatattgat agcctgaatc agtattgatc tgctggcaag aacagactac tgtatataaa
     1561 aacagtataa cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa
     1621 ttgtgacttt tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag
     1681 attacgttga acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt
     1741 acttcgtcaa agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac
     1801 tgattgtcga tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg
     1861 gcgagtttac ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca
     1921 gtgcgtactc gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga
     1981 tccacgtcgt taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc
     2041 cagctgtgag acggtgtttc gccctgattt atggggtaaa ccggtggttg tgctatcgaa
     2101 taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg
     2161 cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt ggcgtggttt gctttagcag
     2221 caattatgag ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc
     2281 gccccgcgtc gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa
     2341 ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca
     2401 tcttactgtt ggtgtgggga tcgcccagac caaaacgttg gctaagcttg ccaatcatgc
     2461 ggcaaaaaaa tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca
     2521 gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag
     2581 caaaaaactg gacgcgatgg ggatcaaaac cgttcttgat ttggcggata cagatatccg
     2641 gtttatccgt aaacatttta atgtcgtgct cgaaagaacg gtgcgtgaac tgcgcggcga
     2701 accctgtttg caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc
     2761 gtttggtgaa cgcatcacgg attatccatc gatgcggcag gccatttgta gttacgctgc
     2821 ccgggcggcg gaaaaacttc gcagcgagca tcaatattgt cggtttatct ccacgtttat
     2881 taagacgtca ccatttgcgc tcaatgaacc ttattacggc aatagcgcgt cggtaaaact
     2941 gctgacgccc actcaggaca gcagggatat cattaacgct gctacgcgat ctctggatgc
     3001 catctggcaa gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag
     3061 tcagggagtc gcgcagctca atttattcga tgacaacgca ccgcgccccg ggagtgagca
     3121 attgatgacg gtaatggata cactgaatgc taaagagggc agaggaacac tctattttgc
     3181 cgggcagggg atccagcaac aatggcagat gaagcgtgcc atgctttcac cacgttatac
     3241 aacgcgaagt tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagcgctcc
     3301 cgcaggagcg ccgaagggat tagcgaccga acaggtcacg ttttttcgct ttaaacggct
     3361 gggaaatcac caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact
     3421 gcggcatttc cagacctaaa aaatcccact gacgctcggc acaatcgcca gaggcgacaa
     3481 acacttgcgg cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac
     3541 aggtggcaaa cggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac
     3601 cgcggaacgc actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg
     3661 cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa cgcacaacgt tcataaatac
     3721 agagcacgca aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg
     3781 ccagagcagt aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa
     3841 atcgcaacat aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt
     3901 cgctgcgcca ccaggaggca gcaaaaatag gaagcaattg gacaattatc gggcgaaaag
     3961 gcaaaccggg catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt
     4021 tgcataaacc attcggttac aggggcaagc gtaaactcga cgcagagcaa gccgacgagt
     4081 gtcagtacga gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc
     4141 aatggcgcga gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg
     4201 acggagggca gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac
     4261 gtgatagcgc cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag
     4321 acgttatccg aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca
     4381 tgttccgatg cctgcaacac aaactgaata attggcgaaa acagttgttg gtcgataatc
     4441 accgcgacta ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag
     4501 gctttaccga tagcatgttc atcggtgaca ccggtcaatg atgtcgccag aataatgact
     4561 gacaaaccaa tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg
     4621 ccaataatcg cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt
     4681 tgatcgtcaa actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca
     4741 aaccaacgca gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg
     4801 gtcaccggcg acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg
     4861 atcaggttct gtggttcgcc caccatggtc atcacgccgc ctaatgcagt gccgacaccg
     4921 gcatgcatca tcaggctacg cagaaagcca cggaactgtt ccagaaccac tttgtaatgc
     4981 ttgtcgatat gactatcgtc ttgcaggtcg gtatcttcag tacgggaaga ggctacgcgg
     5041 tgataaatac cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca
     5101 tcgaggaacg cggagaggaa cgccgccgcc acgcaaaaag agagcgacag cagcattttg
     5161 gagcgaatgc ttaacagcaa acgggtaaat atgaacagca atagctgttt cataaaatag
     5221 atacccgcca ccataaacat cagtaacagc aagacttcaa gatttgccgc cacctcttca
     5281 cggacgtgtt ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg
     5341 gggagcagcg ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc
     5401 aaccagcccg cgacgaaagg gctgatgagg aaaattaacg ggtttacgat taagaaaatg
     5461 atgagggcga gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg
     5521 ccccaggaga tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttatg
     5581 tttaaacgca aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa
     5641 agagatgcgg aaaatgaagc cttgatccct tttttcttct ttttgtctgc tatcagcgta
     5701 gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat
     5761 ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg
     5821 gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat
     5881 tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt
     5941 gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaacct ctggtttaaa
     6001 tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt
     6061 gctgtcggtg cgtaccaata tttcaactat ttttattcgc accgcgtttc gtcagcatcc
     6121 cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgacgcctt
     6181 tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta
     6241 cggtctgatt cttaacggga tgaaagggct gtatacgcgt attggtcgtc actatttcgc
     6301 caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag
     6361 tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga
     6421 gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc
     6481 ccttccgttt aaagagcaaa cccctcaaac gaggggtttt ttgttgtttt tacagatttc
     6541 ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga
     6601 gcatcacatc aaatccccac aggcgatgca catgcttcag cacttctttg cgcccccgat
     6661 ccagcggtgc gcgattatgt ggaatgtaac gcagcgtcag cgaacggtcg ccgcgcaaat
     6721 ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc
     6781 ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat
     6841 gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa
     6901 actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc
     6961 agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa
     7021 tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc
     7081 acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca
     7141 taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga
     7201 aggtcgccca gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca
     7261 ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct
     7321 ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg
     7381 ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat
     7441 actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt
     7501 tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt
     7561 catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat
     7621 cgacaatcga actggcgtcg gtccaactgc ggaataagta attgttttta aagaaagagt
     7681 tatgcccata gcaggcgtgt gccatcacca gcgcctgcat ggtaatggtg ttctcttcca
     7741 tcaggtaagc gatacacggg ttagagttaa tgacgatttc ataggccagt ccttgctgac
     7801 cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt
     7861 taattggcat gccgacgctg gagtaggcat ccatcatctg ttctgaggtt atcacttcaa
     7921 tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat
     7981 aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat
     8041 tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag
     8101 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     8161 tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat
     8221 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
     8281 tgagccagct caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag
     8341 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
     8401 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
     8461 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat
     8521 gccgggcaaa tctcccccgg atatgctgca ccgtgggcgg caccaggtgt gcctttaaaa
     8581 gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
     8641 ttccagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
     8701 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
     8761 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
     8821 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
     8881 tatcagctgc tggaatccag ccgcctggcg gaagtggagc ccgcgctggc agaagtggcg
     8941 cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg tcagctattt
     9001 acccagaatc tggcgcggat ggcggagcag gcgggggtta aattccgctt taatacgccc
     9061 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg cgatgaagtg
     9121 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
     9181 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag
     9241 gaagatggtg cgccagtatc cactattctt gatgaaacct acaaaatcgc cattacccgt
     9301 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgaactg
     9361 ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt
     9421 catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg
     9481 gttgtcgggc gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc
     9541 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
     9601 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
     9661 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
     9721 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
     9781 gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg
     9841 agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta
     9901 cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat
     9961 ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa
    10021 gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg
    10081 atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg tctggcagca gttgcgggca
    10141 atggcgaatg ttggcgagat gaccctgatg tcgcattttg ccgaagcgga acatcctgat
    10201 ggaatttcca gcgcgatggc gcgtattgag caggaggcgg aagggctgga gtgtcggcgt
    10261 tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg
    10321 cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc
    10381 ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg
    10441 ggtgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt
    10501 gtcgccgcag ggtacgccga cggttatccg cgccacgcgc ctaccggtac ccctgtttta
    10561 gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct agcggtcgat
    10621 ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc
    10681 aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcgctg
    10741 gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg agacagcgtc
    10801 ttctggttgc gaaaaaatca tcaatccggc tggtcagcaa ctgtagttgt taatgtgaca
    10861 gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa
    10921 ctcaccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc gaactcgact
    10981 tcagataaat cacgctttac ccttgatgga gcctgtacat agatttgtgt aattgcctga
    11041 ttttgatatg ttcaattcaa catcaaatga aggttaaatt atggacgaca aacaattgca
    11101 ggctcaggct gcgttcagca aagcatcgca accggcgata gatgcttcat taaatttaag
    11161 attcagcttc ctcttcagcc acccgtacgc caatcttcaa cacttcatta tcttctttct
    11221 cggccaccgt ccagatcatc ccggcaaact ctacctggtc accgacaacc ggtgccgcgc
    11281 ctaacaactg ctggacaatt tcacccagcg tttgctgctt atcacgatat tctcgcccgt
    11341 cttccaggcc atatatcagc gcaacatcag catatttggc gctggcttcg agaatgaagt
    11401 caccaaagaa gcgttgatcc agcgcgaccg gcggcgactg gctgaacagt ttaccgagcg
    11461 ccggaagatc gcgttcccga ccaattacac acaacacatc gccttcgcgc agtcgggtgc
    11521 tgccggtagg atgaagcaac tggttatcac gaaacagtgc cgcaatacgc gtctcttttg
    11581 gcatatgcaa atcgcgcagt gccgcgccca cgcaccattt atcggcactc aattgataaa
    11641 caaactgctc ccacggattt tccggatgaa tatccaggcc aacgcgtgac accggacgtc
    11701 ccactggcgg aaccaccact ttggcttttt tagccgccca cgagagtgat gttccctgca
    11761 agagcagtga aaccagaacc acaaagaagg cgacattaaa gaacagacgt gcattctcca
    11821 gccccgccat catcgggaac acggccagga tgattggcac cgcgccgcgt aatcctaccc
    11881 agctgataaa cacgcgctca cgcagattga aaccgcggaa ggggagcaat ccagcaaata
    11941 ccgaaagggg acgggcgaag aatatcatcc atgccgacaa aatgagcgcc ggaatggcaa
    12001 taggcagcag atcgcttggg ttaaccaata gccccagcac caggaacatg gcgatttgcg
    12061 ccagccaggc gaggccgtcg aaattttgca ggatgccgta gcggttgcga atcggccgat
    12121 tacccagcag aaaaccgcac agataaacag ccagaatacc gctgccttcc agcgccgtgg
    12181 ttaaagcaaa aattaaaata ccgccgctta atgccagcaa tggatataat ccggcaggca
    12241 gggcgatgcg attaatcatt tgcagcagta aataaccacc gccaagtccg atgacaatcc
    12301 cgaggccaaa ttgctgcaga atatcgacca cgaacatcca actgacactg ctttcatgtt
    12361 gctggatcat cgcaatcagg gtaatcgtca gaaagaccgc cattggatca ttactgccgg
    12421 attcaatttc cagcgtcgag ccaacacgtt cgttaagccc cttaccaccc agcagagaaa
    12481 agaccgctgc agcatcggta gagccgacga tagcgccgat taataagcct tcaatcaaat
    12541 caagattaaa cagccacgcc gccatcatgc cggttaaacc agaggtgata agcacgccca
    12601 gcgtcgccag cgacagtgcc ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca
    12661 tcccgccgtc gagcaaaata atcgccagtg ccaggttact taccatgtag gcgaaggggt
    12721 aattatcaaa cgggataccg ccgacgccat cgactcctgc cagcatgccg atcgccagaa
    12781 aaataaccag aataggaatg ccaagacggg aagaaaatga actaagtaat atactgctgg
    12841 tgacaaggat ggatcctaag atgaaaaggc taattattgt tgtggcatcc aacggtcggt
    12901 tactcctgat tacgctgtct cttatataaa ccctaccata ttagcggcag agacagcgtt
    12961 ttacttagcc ctgaagcgtg atttttttac attttaagaa caggatgacc gctaatcgtc
    13021 aactgagtgc cttcccgggt gttattcaga atggcatgtg cgcccaacgg cagcgtaacc
    13081 gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc
    13141 aaaaacgcgt acactgactc caggttgtaa cctgcgtcat aatcattggg cgtgctgccg
    13201 ctaaagctac cgagaataat cgccttctga cgtggcaaaa ttcccgcatg atagagctgc
    13261 aacagcatac gttcgacgcg gaaagggtgc tcgttaatat cttccagcac cagaataccg
    13321 ttctcaattt ttggcatcca cggtgtacca atcagtgaaa tcagcatcgc aagattgcct
    13381 ccccacaacg tgccttcggt ctgacatgtc gggccttccc cttgccattc aatggtgaag
    13441 gttttattgc gtaacgccag ccagaaatgg tgctcggtaa aggcgttcag ttcatccgca
    13501 ccaaagtttg ctaccagcat ggggccgcta aaggtgatga cattgccttg cgccagaaga
    13561 ccgcactgaa tggcggtaaa atcgctatgt ccgcaaatga gtaacgggtc atgttgttgg
    13621 cgagcaacca gtgcctgcca gtcaatatct gccagtaaac gactggcacc gtaaccgccg
    13681 cgtacagcca gcacgatggt gttgggggta gtcagtctgg caagggaatt gagatcttcc
    13741 aggcgctccg tttccgtacc cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta
    13801 ttgacctgat gcccctcgtc ggtaaggcga tggataccac gcagcgcggc gtgctgttta
    13861 atgcagtaac ccgatggggc aattaagtga aacagagaca tggcaattcc ttgctgacaa
    13921 cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc
    13981 aggctataag gatagaagaa gtgaaattga gatggtttgc ctttttgatt gtgttattag
    14041 cgggttgttc atcaaagcat gactatacga acccgccgtg gaacgcgaaa gttccggtgc
    14101 aacgtgcgat gcagtggatg ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc
    14161 cacaattgat cacggcgatt atcgctatcg aatcgggtgg taatccgaat gcggtgagta
    14221 aatcgaatgc cattggtttg atgcagttaa aagcttcaac ctccggacgt gatgtttatc
    14281 gccgtatggg ctggagtggt gagccgacga ccagcgagct gaaaaatcca gagcgtaata
    14341 tttcaatggg ggcggcttac ctgaatattc tggaaaccgg cccgctggca ggcattgaag
    14401 atccgaaggt actgcaatat gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc
    14461 tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg
    14521 acgagttcct cgatcacgta gcgcgaaatc atcctgcgcc gcaggctccg cgctatatct
    14581 acaaacttga gcaggcactg gacgcgatgt aaatcagtcg cgcactttgt ccgctttttc
    14641 ccgggcttct cgctcgagag agaaaataat ccgctgtaat tgccgctcca ccgtcgggct
    14701 gacgttaaga aaacggaagc tcagacgggg agtggtgatg gtttcattct tgccatcaat
    14761 cactttgcgc tcgctgatgg agattaactg ggcgtcaaag tgaaaaacac cccattgccc
    14821 catgttgact tcaatctgag cgaagcgcat gccttcttgt aattcggcag gctttgctgt
    14881 ttccagtaat gcgcccatgc cgcctaacga caaatcatac aggcggaaac gtaacgtact
    14941 gttatccgcc agtttggtct ggcaaaaata aggcggatgg agtggggcgg agatgcggaa
    15001 atatcggcgt cgttgtacaa accataaggt gggaggcggt acggtgataa atgccggaag
    15061 ctgcaagtat tcactctgct gtagttgttc aacagtaaac tcgactttcg caccctgagt
    15121 ttcggcggta atggtaatgt gctgtgcctt tagcacggcg atgttgtctt cggcttgact
    15181 gccgaaatcc agcaccagtt tatccggggt tattgccagt attttgctga tcagctgccc
    15241 gccattccaa ctgagacgca aaggaattgc ggctttgtgc aaatcgcgta acacgcccag
    15301 gacggctaac ggattttgtt tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc
    15361 ctgactgaca aactgtcttt ggtttatcgg tcacagttaa caaaacttaa tacaaatgcg
    15421 tgaatatttt ttacatgttg ttcttaaatc agccgacata cgcccagcat cgatcccgcc
    15481 cttgcctata cttagagcgt tgacgtaagc atttcttgcg tccgattcat cgaaacgagg
    15541 gcctgaacat gggaattatt gcctggatta tttttggcct gatagccggc attatcgcca
    15601 agctaatcat gccggggcgt gatggtggtg gatttttcct gacctgtatt ctcgggatag
    15661 tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg cattggcggc tccatcagtg
    15721 gttttaatct gcacagcttc ttggtggcgg tggtgggagc tattctcgtt ctgggcatat
    15781 tccgcctcct gcgaagagaa taagattttc ataaggcgga tagcgataca gatgccgcta
    15841 tccgctttca catcagaacg tatactcgac acctgcttta cgggtgaaaa aaatcaatat
    15901 agcacttcga tatccgataa agtcggttcc cggtaacaac aacagaatcc ttttttaatt
    15961 attgtttcgt tgtttttggt gtgattcaga aaataaatat tttagacagt agccagtcag
    16021 gcgttaaaaa tggggcggat agtgaggcgg ttttggctgc gcttattgaa catttagaat
    16081 tgatcaatcc ttctggcaga ttaagccaaa atacccgttc ggcaatgctt cagttacgcg
    16141 aagaggaatg gtctgaattc tttttttggt tgctaaattc tctggagtgt ctggattatg
    16201 tgattattaa tttaactcca gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata
    16261 ttcaggtagc aattgatgct ttatatagcc aacgtcggcg taaatcgccg ggtgatgaaa
    16321 gtgaaacact tacccgtaga aatgacgcta tcttcggcaa ccacgtatgg caaacatttg
    16381 cgcaatactt cccgccagga cttgaaaaac cgtctgtgta gaaaaaacca tagctatggt
    16441 gttatctgcc gacaacgcca tccggagaag ccgaatgacg ttgtcggagt aagcgctggt
    16501 taaggtgtgg gttgtgcctc tttggttgag ggttgcgtcg ttgctgactt aacggtcgga
    16561 cgcgtcgccg gaacattgtc acacggttgc tctttcgggc agatcaaatc cagcattttc
    16621 agcgtcacgc cattggtcca gccaaagcca tcctgtaatg gatattcgcc accgccgccc
    16681 cccgttccgg tggtgctgac atcatatttt tccaccagct ttttctcccg gtcataggtg
    16741 tgctgaacat tggtcaggaa gtgccagcta atgtccatcg ccacctcttt ttgcccgtag
    16801 ttttgtaatc cttctgtcgc gacccactgt aacggtgccc agccatttgg cgcatcccat
    16861 tgttgcccac ttttcaccga cgtggtgttc aggccgccgg gttgcagcag atgtgttttc
    16921 gtcgccgtcg ccattttgtt ggcgcgatct ttcgctgccg cattgacgta cagcgggaac
    16981 agggcggccg cggttaactg attgcgcact ttatgacttt tcaggtcgta atcggcatac
    17041 cagccttgtt gatcgttcca catgtatttt tcgatccctt tttgacgggc atttgccagc
    17101 gtttcgtact ggtttgccat cgcgttatct ccggcagctt tgctggcgcg ggcgaggatt
    17161 ttttccattt taaacatcag gctgttcaga tcgaccggta cgatgctggt ggtgcgtaag
    17221 gtatttagtt gatgcgggtt gtccatccag cgcgagctga aatcccagcc agacgcagcg
    17281 gcagagcgca ggtcgcggta aatttcagtg gcaggtcgat tcggattgct tttggcggtg
    17341 gcaatatctt ccacccatga ctctggtcgt ggcgtatcgc gatcgtccca gtagcggttg
    17401 agaagggtac catcctgaag ttttacaacg cgtttttcct gttgtccggc ttgcaggttt
    17461 tcaacaccgt ccatccagta agcatattct ttttgcattt gcggcaggta ttgcttcaac
    17521 gcggcatcgc cttcatgctg cgccagtaac tctaccatca gggcaaagaa gggcggttgc
    17581 gagcggctta aatagtaact gcggttgccg ttgggaatat gaccgtaagt gtctatttca
    17641 tgagcaaaat tggccaccat atccgcgact ttatcccagt gaccgctttc ggcaagtcct
    17701 aacatggtga agtaactgtc ccagtaatat acctcgcgaa aacgtccgcc cggcacgaca
    17761 taaggttccg gcagcggtaa cagagaatcc catttttcgg tgttttcggt agaacgcgtt
    17821 aataccggcc aaagtccgtc aatatgttcg cgcagtgact gcccctctgg cggaacatat
    17881 ttctcgccct ctttcggcag ggtgaaattg acgttaacga aatggcgcag atcaaatccg
    17941 ctctggtttt gctgcatccg ataatcagca aggatcatca gcggatcgct gttcggcacg
    18001 gcatcggcaa aggttttttg gtccggaaaa agtttggcgt tttgcacatc attaaacagc
    18061 ggccctaata aaatatcagg cggctgtggt gttaccggtg tttcttctgc ctgcaccgat
    18121 agcgcagcga aacacaaaaa gatacaggct ggaattaacg ccattttttg cgggcgagaa
    18181 ggtgcggggg atttcatcaa tcattctcct ttggcgaaac cgaataaaac ggtattcaag
    18241 tatcagaaaa ccttagttca ggatcgcact agctgcatga tcgaaatctc attctgcgaa
    18301 ctgtcaggca ttttagaaaa gggaattaaa gggataaata acggtgaaat agagtgatca
    18361 aataacgtct gtttattagc cagtcagcga cgttgggttc gcgattagcg aagaaaaatc
    18421 atcataaatt ggtaaaaaat ataacaggta gttaaaaaac atcagtgctg ggtaaattgc
    18481 cggatgacat cagaacgatg ccatccgaac ggtggcttaa ccctgacggt tgaaacgttg
    18541 cgttttaacg tccagcgtta gcgtttcttc tggttgtatc gcatacagtt tctcaccctg
    18601 ctggcaaatc cagccaatcc ccagttcacg ggcgattagg gcgctgtggg ataccggact
    18661 tccggcgcta aggcagatac cttttacaac cgccggatcc agttgcagta ctgtggaagg
    18721 ataaatgttc tccgccagta gaatagttgg cgagttaaac tgcgggagtt cttctttcgt
    18781 ttgggtcagg tggaccaggg tgcgatgcag aagatcgtcc acatcaatat agcgagcttg
    18841 tagatattca tcatccagtt gctggtattg ctggctaagt tctttaagaa cttgctgcca
    18901 ggcatattct gccgtgcaat gttcatgctg aaggagttcg cttgccgccg ccagcagttc
    18961 cggatcatct aacagtgtat ggtgaccaga aaagattgcg gcaatatcgt caagcccgct
    19021 ggcttctgct tttgctgtta acgtcatcag atctaataac gtgaagtcaa tagcctggcg
    19081 taatcgatct tgttcttctt ccacggtcag ggttgatttt gcctgtaccg tacataaaac
    19141 tggttgataa taaaaggctt tacccgaaac aggcggaacg ggacgcagag taggtggagc
    19201 gacttcctcc gtttcaccaa agttatcttc agccagctga cggaaagcga tcagtgcctc
    19261 ttcagcttct ggccctttcg caatcaggcg cagcgtatcg ttatagcgaa cttgtagtaa
    19321 cgcaatctgg ttaatactct ctggtgtgac gcatttgccg tttttttcca gcaacatatc
    19381 ggcattaaat gtcgataagg tataaaccag ccgggaggcc ggacgtacat gcaggccgtt
    19441 acggtttttt atgaccaccg ccagagaacg ggcttcttca tcgtacgcag gacatgtgtc
    19501 agagatttta gtgtcggagg acggtaaacc cagttgttca cgtttggctt ccagcgcatg
    19561 catagcgtca aagataactt tgtcgatatc cgctcccgag gccgcgctga ccgttgctgc
    19621 cagtgtacct tcgaccaacg gcgcagcaca caaacgtact tttgcggcga tcttgggagc
    19681 cagcaattcc agcgcagttt cagcactcaa taatgcgcta cccatatcca tcatgaccag
    19741 cacatggtcg gcatcagcaa cagattcgat ggcctccatc actttgacgg catcggtacc
    19801 aataggattt tgtggatcgt caattcccgc ggcaatggcg attttacaac tatcactcat
    19861 taacatctga cgggctaatt caccgacacc ttctcccagt cggctgctat gtgaaactat
    19921 gaccaggttt accatcacca attccttact cttttgcggc taacgccaac atttgcatca
    19981 taaacatcac cgaggtcgcg ccgggatcct ggtgaccaat actgcgttca ccgagataac
    20041 tggcgcggcc tttgcgggct tgcatcgtaa tcgtactttg tgcagcggat tcggcgattc
    20101 tactggcagc ttcgagcgcc accggaacag agagattttg ctcgctggac tgacgtaacg
    20161 attccaccac cggcacccac acatcacaca tggttttatc gccaggttcg gctttcccgc
    20221 gactgattac gtcgtccacg ccatcgcgga acatctgata aagctcttcc agtgtcaggc
    20281 tttgccgtgc ctgggtcgcc tgtgcggcgc ggataaagaa ggtaccgaac agcggaccac
    20341 tggcaccacc gacgctggaa agcagcgtca taccggtatt cttgagaatg aaaccgatat
    20401 ctttatctgc gatagcaggg agtttttcca ccactttgct aaagcctcgg ttcatattta
    20461 gcccgtggtc agcgtcgcca atttcgcgat ccagtccggt aagatactcg ctctcggtgc
    20521 tgaaaatatc gccacaacga gtgagccagt taacaatttg agttctgctc agtgacattg
    20581 ctttctcctt atttacccca gttaagggcc ggggtgtgga ccggggcgtc ccagagtgcc
    20641 agcgtttcgt catcaacttt cagtaaggtg attgagaaac cggtcatatc cagtgaggtg
    20701 cagtacgcgc caattaaatt acgttcgata gtcaatcccg cttgctggca acgtgtggtc
    20761 aggcggttat agacgccgta cagctcagaa agcggagttg cgccaagatt gttaaccagc
    20821 gcaatcaccc gatcgccaga ctggagcggt tgtttggttt gttgttcttc ctgccaactg
    20881 ccttgttgat aatcccagaa acgcaaagtg cgatggtatg agccattttc cagcagggtg
    20941 tcgaacattt catcgacggt ttgatcaagg gaagagaagg agcggcggtc aatacccggc
    21001 tcaccatgaa tgccgacgcc aaactccatc tcattatccg ccagggtaaa agaaggtttg
    21061 cccgcggcag gaacggtaca ggcaccgaga gcgataccta ttgagtggcc ttgattattc
    21121 aacttacgcc ccagttccgc acaggcgtcc agtgagtcgc cacgctccgc cgctgcgcct
    21181 acgagttttt caattaatac ggtgttggca acgccgcgtc gcccggcggt ataaagactg
    21241 tcttttaccg caacgtcgtc atcaatgacc acagtggtca cttttacgcc gctatcgtgc
    21301 agtaactcgg tcgctgtttc aaagttaaga atatcgccgg tgtaattttt gataatcaac
    21361 agtacacctt cgccgccatc aatttgcatg gagcattcaa agattttatc gggcgtcggt
    21421 gaggtgaaaa tttcgcccgg acaggccccc gaaagcatcc cctgaccgat ataaccacag
    21481 tgcatcggct cgtgtccgct gccgccaccc gacagcaggg cgacttttcc tgcaacaggg
    21541 gcatcagctc gggtgacata caccggatcc tgatgcagtg tcagcgatgg atgcgctttc
    21601 gccagtcctg ccagttgttc gtccagtacg tcttgcacat cattgatcaa ttttttcatt
    21661 attttgctcc agcaattacg gtagggcatg gatgatgttc aacgacacgg cgaccactga
    21721 ctgccgatga atccattgtg catcaggcaa gggaaaagaa aattagccca aattatgttt
    21781 catagtgaaa catatgcttt aatgaatgtt ccatattgaa acttttacgt gtattaatac
    21841 ttaaaattgc gagccggaac accttttgtc ataagggatg cgggatatga gtggcgcttt
    21901 taacaacgat ggtcggggca tatctccctt aattgcaacc tcctgggagc gatgcaataa
    21961 gctgatgaaa cgggagacat ggaacgtacc acatcaggcc cagggcgtga catttgcttc
    22021 tatttatcgg cgtaagaaag cgatgctgac gctcgggcag gctgcgctgg aagatgcctg
    22081 ggaatatatg gcaccgcgag agtgtgcgct gtttatcctc gatgaaaccg cctgcattct
    22141 cagccgtaat ggcgatccgc aaaccttgca gcagctaagt gcactgggat tcaatgacgg
    22201 cacgtattgc gccgagggaa ttattggtac ttgtgcacta tcgttagcgg ctatctctgg
    22261 tcaggccgtg aaaacgatgg ccgatcaaca tttcaaacag gcactctgga actgggcctt
    22321 ttgtgcaacg ccgttgtttg acagcaaggg ccgattgacg ggaacaatag cgctggcgtg
    22381 tccggttgag caaactaccg cagctgattt gccgttgacg ttagcaatcg cccgcgaggt
    22441 cggtaatttg ctgctgacgg acagtttgct cgctgaaact aaccgtcatt taaatcaact
    22501 taatgccctg ttagaaagta tggatgatgg cgtgattagc tgggacgagc agggtaattt
    22561 gcaatttatt aatgcccagg cggcgcgggt cttgcgcctt gacgcgacgg caagtcaggg
    22621 aagggcaatc actgaactct taacgttacc cgccgtattg caacaagcaa taaaacaggc
    22681 acatccgctc aaacacgtag aagcaacctt tgaaagtcag caccagttta ttgatgcggt
    22741 gataaccctt aaaccgataa tagaaacgca gggaaccagt tttattttgt tgctccatcc
    22801 tgtggaacag atgcggcagt tgatgaccag tcaattagga aaagtcagcc ataccttcgc
    22861 tcatatgcca caggacgatc cgcaaacccg ccgcttgatt cattttggtc gccaggcggc
    22921 gcgcagtagc tttcctgtcc tgctttgtgg agaagagggc gtgggcaagg cactgctaag
    22981 tcaggcaatt cataatgaaa gcgagcgtgc tgcaggtcct tatatcgccg tcaattgtga
    23041 gttatatggt gatgcggcac tggcggaaga atttattggt ggcgatcgca cggacaatga
    23101 aaatggccgt ctgagtcggc tggaactggc gcacggcggc acgctgtttc ttgaaaagat
    23161 tgaatatctg gcggtggagt tacagtctgc tttgcttcag gttatcaagc agggtgttat
    23221 cacgcgactg gatgcgcggc gcttaatacc aattgatgtc aaagtgattg ccacaacgac
    23281 cgcggacctc gcaatgctgg tggaacaaaa tcgttttagt cgccagctgt attacgcgtt
    23341 gcatgcattt gaaattacca tcccgcctct gcgtatgcgg cgtggcagca ttccggcgct
    23401 ggtgaataac aaattacgca gtcttgaaaa acgcttctct acgcggctga aaattgatga
    23461 cgatgccctc gctcgcctgg tttcctgtgc atggcctggc aacgattttg aactttacag
    23521 cgtcatcgag aatcttgctc tgagtagtga taatgggcgc attcgcgtca gtgatttgcc
    23581 ggaacatctg tttaccgagc aggcaacaga tgatgtcagc gccacacgcc tttccaccag
    23641 tctgtcattt gcggaagttg aaaaagaggc aattattaac gcagcccagg tcacaggcgg
    23701 tcgcattcag gaaatgtcgg ctttacttgg gatcggccgc actacgctgt ggcggaaaat
    23761 gaagcaacat ggcattgatg cagggcagtt taagcgccgg gtatgaaaga cagagacgat
    23821 ttctgataca tcagagtgat ctatatttca ttccggcgca cgctaacatt tttcagcatc
    23881 gtttaagggc tggtctatcc cgcacttaaa agctgaagcg atatcctccg ttgacttgct
    23941 tctgatcaaa gaggttactc tgcgtgtaat ccgcttcgag gtagaatgtg tgctgtttgt
    24001 tatactgtgc actgactccc acgccgttat tccagccatt acctttgaaa ctgtacttct
    24061 cccgggagtt gttcaacaga tagtctgtat cccctgaaaa ctcgcggata gctccagtct
    24121 tcacatacat attcagctgg ctgttgcctg cggtgatgtc atatcccagt atcatgctgg
    24181 cacgccccag cagcgattcg tagtgattca gatgtatatt gagaccatta ctcgccttca
    24241 tagccatctc attctggtgg ctgtatgtga gctgggtttg cggctctata tagaacccat
    24301 aaccagtagg ggtcaggttg aacctctgcc cggcttccag ggagatgctc aatccattcg
    24361 cagtgccgtt ggcgttaacg ccgttgttct gactgtccag tacgtgaaaa ctatttttct
    24421 ggcgcgatgc ttttacaacg agatcgctgt aaaaaccgtt gtgtgccatg taactggcat
    24481 acattcccat gtagtctgaa cgtgcggtac cgtcgcctcc gctatagtcc ggcgatgcat
    24541 gtgttgagcc aatatacaga ccgacataca acggcattac atcagagaga cgtttatccc
    24601 caccaaactg gataccgctg taacccatgt caaagccgct cagtttgcca ctggcgaagg
    24661 agtccaggct tcccccataa ctgcgcagcc agatattacc gtctttactc tgatttcgca
    24721 ggtcacccat tcgttgcatc agcgtacggt tttcaacata gtttaataag taaccgacat
    24781 tgagataatt gccgccagca tctgcggtcg tggtgggttt aggtgtggga gtggggttag
    24841 gcgtaggatc gggttcaggc gtaggatcgg ggttgactat gggaggctga gcgggagccg
    24901 gtgttggttc aggattagga gtcggttccg gaactgtccc ggaagcgtaa agctcccagt
    24961 tagtgccatt tttacgcaca tcgtacagat atccccccaa ctcaacctga gaagacgcgc
    25021 tgaacgaggc cgcaccgtca gtggttttta ccactgtcag aacttcattt cccgtagtgg
    25081 cctcgctgcc ctggttgcgg atagccaata cgtgattacc agcactactt ccgctgatat
    25141 tcaataaatc ccctttatta ttaacgccat taccctcgcc aacaacatca gcacgcataa
    25201 taaaggtact gttaccgctc aggttctcta cgtttaatgt ggcaaatgtg ccggcagttg
    25261 acccgtggct ggcaaaatcg acagttgaat ggctcagcgc cagcgtgtcg agattagaat
    25321 tacttgttac gttccagacg ctgttattca ttgcaacgtc cagtttcccg ccattgacat
    25381 tatcgctgag ggaggaacct gtccaaaccg accctgggtg catatctaga ttgattagcc
    25441 cacctttgga aagaacgcta ccgttgataa gcatacgacc cgaggcgttg atgcggctgg
    25501 cggcataacc atcgttatgt tgcgttgcaa tggccatctg gtcgggtgta ctcatatcaa
    25561 tgaccagatc gctcgtgagg tcgatctggc tgttggtcat ggcataaatt cccctggctc
    25621 ctgccgcgcc ggtgatagcc aaactgtctc cggttattct accgccgctg agcgcccaca
    25681 aacccagcgc cagactgcca ttacgatcaa ctgtaatatc ggtattttgc atgttgacaa
    25741 cagccgttgc cgtctgggcc gaggcaccat aagaaccgcc ggaaaagatg ctgtttcgtt
    25801 gcgctgccgt gccagaaaaa ttgattgtcg cgtctgaacc actggtgacg aggccaccgc
    25861 cctgcgcgga agaaatatgg ctatctgcac cgatagtggt tgtaccacca cgaacttcga
    25921 cgccattggc cgcggctcca gttacatcaa cagtgagtgc attcgcgcta acctggccaa
    25981 agctccagag gccgtgtgca ttatcgccat tggttttaat ggtactgttt gttccgagat
    26041 cgacaacaga gtttttctgt acgtttatcc ccatggcgct gtagccctga acatcgattg
    26101 tcaggtctgt cgccgtaaaa cgcgcagcac cattggcgtt attgccgttg agaccaccga
    26161 tataaacacc tgtacttcca tcggtcgtga ttttacttcc gcttccaaga tcgacactgg
    26221 tgccatagtc attgatggtt aggcctatac cgttcgagtt ttcaatggtg aattgagtgg
    26281 ctgttagtgt tgagctatgc ccaataatga tgccgtcatc gttactctta atggtgctgc
    26341 ctgtgcctaa gtcagcatgg gtatagtcac caattaagtt aatgccgatg gcagaggttt
    26401 gaccaacaac atctactgtg agtcggttag ctgtcaggga gctgtttttg ccctgaataa
    26461 tgattccttt tgcatatcca gaggcgtcgt tgacattgac ggtgacatca ttacccaggt
    26521 tgatattgcc aggcgtgttt cctgctgggg tcattacacc atagaggttt cctgatggat
    26581 cggcggtatc tcctgtaatt ctgtcaccat cattaatagt aatattggtg ccatattgac
    26641 ttcctgtatc agcagctatc agcagggggg aaaagataat tgcattcaaa ccaatagcgt
    26701 caaattttat cagttgaatt gagggcgaac ggatttttaa ttcagcgaga cgatctgctt
    26761 tggtattccc attgtgttgt ttgatgccca taccactcct atatagtacc cagactatgg
    26821 gtctatggat tttgtcattt tgtttaaatc aatgaataat atcctcttat catcatgata
    26881 tttgtatggt gcaatcatga tgagcttctc agagaataat tctctattac cgggcaaaat
    26941 caggaacaca ggaattaata tatatttaac atccccctta caaaggtggg tttatatcga
    27001 gttattccct aagttgtcgt tgcttattcc aattcgttac tataaatatg gcaaaaatat
    27061 tacaacaaac agcggttaaa aaatttggtg aagatttttt gtaataatcc taattagtta
    27121 tgggaatttc gcttgatgta tctgcataac aaaaacaata tgcgccattt ttgcattact
    27181 aataagaaaa agcaaaaaac atcgatattc atttttggtc aatgggtttt ctggcctatc
    27241 gttgttattg tgctactggt tatcattttc atcttgccag gatcgcacgt aatcacgtta
    27301 ttcggggact ttatctgcgg gatattaccc ttcaggtaag tccttttctc gccagcatat
    27361 tggagcgtga tcaatattga tcagctgtga agggggtgat gtgtagtgga ccaaaactta
    27421 cgttatatca ctactaatga gatatccgtc atctgacact gatgtcttta attattctcg
    27481 tggttcactg taggcaataa gcacaaaagt gtaggatgtt acaagaatga ttaggactcg
    27541 gtgaaatgaa aaatccacgc aattgcgtgg attatatata cttttgcgct cttcatgaga
    27601 tttagcgaaa cctcatgaga caacaaatta attagacgtt gaaaaggaag ttcatcacat
    27661 cgccatcttt aacgatgtaa tctttacctt ctgcacgcat tttgcctgct tctttcgcgc
    27721 cttgttcacc tttgtaagtg atgaaatctt caaacgagat ggtttgtgca cggataaagc
    27781 ctttttcaaa atcagtatgg attttgcccg ctgcctgcgg cgcggttgct ccaaccggaa
    27841 tggtccatgc acgcacttct ttcaccccag cggtgaagta agtttgcagg ttcagcagct
    27901 tataaccggc acggatcaca cggttcaggc ccggctcttc cagcccaagc tcctgcataa
    27961 actcgtcacg ttcttcgtcg tccagttcgg caatgtctgc ttcaacagca gcacaaaccg
    28021 gaaccacaac agaaccttct ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt
    28081 tttcaaaacc gtcttcgttg acgttggcga tgtacattgt tggtttcagc gtcaggaagc
    28141 tcaggtaacg aatagccgct ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg
    28201 cattttccaa ctggggcagg catttttcca ggaccgccag ctcagctttc gcgtctttat
    28261 cgccaccttt ggctttcttc tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg
    28321 ccagcgccag ttcggtgttg ataacttcaa tatcgtcagc cgggttaact ttgccggaaa
    28381 cgtgaatgat gttgtcattt tcaaagcagc gaacaacgtg accgatcgct tcggtttcac
    28441 ggatgttggt caggaactgg ttacccagac cttcgccttt cgatgcgcct tttaccagac
    28501 cggcgatatc gacaaattcc atggtcgtgg gaagcgtacg ttgcggtttt acgatttcag
    28561 ccagttgatc caggcgaggg tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc
    28621 agaatggaaa gttggccgct tcaataccgg ctttggtcag cgcgttgaac agggtagatt
    28681 tcccgacgtt gggcaaaccg acgataccgc atttgaatcc catgatttaa atcaccttaa
    28741 tatcttaata atcaacctgt tattgctaac agattgcaga aatggaaata actttgccta
    28801 ttatacacgg cactcggcaa aaatgccgca gacaacgact tattgcgctt taaaggcgtg
    28861 caatcggttc gttgctttgg tcaagccatc tgtaaaccac atttcagtac aacgcgccgc
    28921 ttcgtcgatg gcttcatcaa ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa
    28981 accgacaact ttatttttat cgcccggatg accgattccg atgcgtaaac ggtgaaagtt
    29041 agggttatta cccaatttac tgatgatgtc tttcagtcca ttgtgaccac catggccacc
    29101 gcccaattta aatttggcga cgccaggagg cagatccagt tcgtcgtggg ccaccagaat
    29161 ttcgtccgga ttaatgcgga aaaaactggc catcgccgca acggctttgc cgctgagatt
    29221 cataaatgta gtcgggacta acaggcggac atcttcgcct ccaagagtga ctcgcgaagt
    29281 ataaccaaag aatttagcct cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc
    29341 aacgaaccag gcaccagcat tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag
    29401 gccgacaatc aatttaatcg tcacgttttt ttgtcctgag tgtgtacata actggcgcgt
    29461 agtttactgg ttgcggcccc gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat
    29521 aattgcatga gtggactatt agaaagtcaa ggtgttcagg cgtttatttg taaagttttg
    29581 ttgaaataag ggttgtaatt gtgatcacgc ccgcacataa cccacagggt gttgtctata
    29641 ctttacacat aaggaagagg ggtattccct gttacaaccc agaaagttcc ggaggtgaca
    29701 tatgaaacgc aaaaacgctt cgttactcgg taacgtgctc atggggttgg gtctggtggt
    29761 aatggtggtc ggcgtggggt attcaatcct caaccagtta ccacagttta atatgcccca
    29821 gtatttcgca catggtgcag tgctaagtat tttcgtcggt gccattctct ggctggcggg
    29881 tgcccgtgtt ggcgggcatg aacaggtgtg cgaccgttac tggtgggttc gccactatga
    29941 caaacgttgc cgccgtagcg ataatcgccg tcatagctaa caaataatgc agtttgctga
    30001 ccagtcagtt tgcgctgact ggtcaatctc gtacttataa atccgccatc gccgcgcgac
    30061 gattcgggaa gaacgccagg cgtcccggga tcggttgaat gccagcgcga gccatagtgc
    30121 gcagtggctg gaattccacg ttgcacacgc gcagttcaca tccctcgggc agacgcttca
    30181 caaaacgctg gaacgcatca agaccaccag catcaagtac cggaaccgca tcccacttca
    30241 gaatcacaat ccgtttgcct tcaagacgtg actccaggtc cgtgaacaag ccttcagcag
    30301 cagcaaaaaa cagcgggcca ataacgcgca gaaccaggac atcgtctgga acatctacga
    30361 ctaccggtgc caggcgagtc atacgtgcga tacgacgcat aaacagcagc gatgccagca
    30421 cgatccccac gctgatggca ataaccatat caaacagcac ggtcagcgac atgcacagca
    30481 gcatgacaat gatgtcatct ttcggcgcat gacgcagcaa gtcgaccact ttgtgcgctt
    30541 cactcatgtt ccacgccacc atcaacagca gggcagccat ggcggaaagc ggcagccagg
    30601 agagcagcgg tgccagtacc agcagggcaa gaataaccag aatagagtgg atcaccgccg
    30661 agatagggga agttgccccg gcacggacgt tagcggcaga acgcgcgatg gcagctgtag
    30721 cggtaatgcc accaaagaac ggagcgatga tattccccag tccctgtcca accagttcgc
    30781 tattcgcctt gtgtttcgtc ccggtcatac catccagcac cacggcgcag agcagagatt
    30841 cgattgcgcc gagcattgcc attgagaatg ccgcaggcag cagtgtgcga atagaatccc
    30901 aggttagcgt gaattctgaa ttaggcagat cccacggcag caccagttgc ggcagcagtt
    30961 gcggaatacc gttaccctga gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg
    31021 tagcaacatg tccgccgagc aggttaacaa tccccatcac cgcgcaacca gccagcaagg
    31081 ccggaaggtg accgggtaaa cgaatgccca gacgcggcca gaaaacaaga atacctagcg
    31141 tcacaatgcc aatggcagca tcacccacat taatggtcgg cagcgccata aataatgcgc
    31201 cgactttttg tagataatgt tccgggacat gggccatttg cagaccgaga aaatctttaa
    31261 tctgcatggt accgatggtg atcccgatac ccgaggtgaa acctaaggtg acggaaaccg
    31321 gaatatactc aatcaggcga ccaaagcgtg ccagacccat cagaatcaaa aaaatccccg
    31381 acagcaaggt cgcaaccagc agtcctgcca gtccaaactg ttgcgacacg ggatagagaa
    31441 ttaccacaaa tgccgcagtc ggaccggaaa cgctaaagcg tgacccaccc gtcagagcaa
    31501 tgacaatccc cgcaacagct gcggtatata aaccgtactg gggtgccaca ccactaccaa
    31561 tagccaacgc catcgccagc gggatagcaa taatcccgac ggttatcccg gcaatcaggt
    31621 cacgggtaaa ccgtgcggca gtatattttt ctttccagca agcgtcgatc agagcgcgga
    31681 aaggcatcac atgtgaggaa aatattttgt tcacaataat gtttcatccg tgagcgcatc
    31741 atctgtcaac taaatggcag gtgaaggagg cataggtcat acaaatggat attacagaca
    31801 aaaaaacccg ccgcagcggg tctttgagcc gggttcgatt agtgttcgaa catggcagag
    31861 atcgattctt cgttgctgat acgacgaatc gcttcggcca gcatacctga cagggtcaga
    31921 gtacgcacgt tcggcagtga tttgatttca tcgctcagcg gaatggtatc gcagacaacg
    31981 acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga
    32041 gtcgcgtacg caaatacacg tttagcacca cgttctttca gagcttcagc agctttacac
    32101 agcgtaccgc cagtgtcgat catatcatcg accagtacgc agtcacgacc tgcaacgtca
    32161 ccgatgatat gcatcacctg ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc
    32221 atatcggtat cgttcagcag cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc
    32281 ggagaaacca caattgggtt atccagattc agctgcagca tgtcttccag caggatcggg
    32341 ctaccaaata cgttatcaac cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga
    32401 tccactgtca gcacacggtc aacaccgacg ctggagagga agtctgcaac cactttcgca
    32461 gtgattggta cacgagcgga acggacgcga cggtcctggc gcgcatagcc aaagtagggg
    32521 ataacagcgg tgatacgacc tgcggaagca cgacgcaggg catcaaccat aacgactaat
    32581 tccatcaggt tgtcgttagt aggggcacaa gtggactgga tgatgaaaat atcaccaccg
    32641 cgtacatttt cattaatttg tacgctgact tcgccatcgc taaagcgacc tacagcggcg
    32701 tcgccgagtg aagtgtacag gcggttggca atacgttgtg ctagttccgg ggtggcgtta
    32761 ccagcaaaaa gcttcatatc aggcacgaga agaacctcag gcatgcgtcc attggtggaa
    32821 agaatctgcc gaaaactgtg cgggccaggc atgatccttt ccaggcggtg tattaaagag
    32881 cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa
    32941 agcatggctc tgtgcaatgg ggaaagatta acgcctttcg ccacaaagcc attgagccat
    33001 tccggggctt gctctagcac ctggcgggct tcagactctg tatcaaattc agcaaagaca
    33061 caggcccctg tcccagtcag gcgcgacggg gcgtattcta acagccagga aagcaccgca
    33121 tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat cattgctgaa ttcacatttt
    33181 agcaacgttt ctattgacct ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc
    33241 accggagtcg gaatacttac accagggtgc gccaccagat accacttctc tggcggatcc
    33301 accggcgtta gtatttcacc aacgccttcg gcaaacgcgg catgcccccg aacaaagaca
    33361 ggaacatctg cgcccagcgt cagccccatt tccgccagct catccatgct tagcccgcat
    33421 tgccagagat gatttaatgc caccaggacc gtcgcggcat tggatgaacc accgccgaga
    33481 ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga
    33541 cgcccgctgt ctgccgcagt tttcatcaac aatcgcgctg cgcgaacgat caggttatct
    33601 tcatgttcca cgccttcaac gggcgttaac agacgaatat ccccatcgtc acgaagctca
    33661 atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca
    33721 tccgcacgct gaccggtaat gtataaaaac agattaagtt ttgccggaga gggccactgt
    33781 gtccgcatta tttcactatc cagttatcca tttttaactt gatgcgttga ccaccgtcgg
    33841 tgagttccat attggctggc atcgcaggtt gcgttttggt gtcataacca ccataaacaa
    33901 ccttccagtt tttgccattc tggctgtagg taatttcgct caggcggtac tggtcgtcca
    33961 gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca ctggcgcaag ctgttgagcg
    34021 gaattggcat tccggtcaat ttgccaatca tctcttcggc gtcatcggcg gtataacgct
    34081 gacctttatt gtcgactaac tgcacgttac ccggttgagc attcagctcc agttccgtgc
    34141 tgcccaatgg gttagtgagc agcagacggt agcgatcctg gccggtttgc tgccagaaaa
    34201 agcgggcgta cactttttgt tggtcagaaa tataagcgaa cgcgccgcga gtctgatact
    34261 gattaagatt gcgcacgtct tgctgatgct gacgccattg tggcgaatcc gggcttttgc
    34321 caggaccttt gggcgtggta acggaacagg cagtgagcac aagagcagcc agcggtagca
    34381 ggcggataag acgaaaatcg ggcaggggca tagtgatgac aagtccttga gatacgttgc
    34441 agttataacc cttaatgcta gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa
    34501 ttgccagaat ctaacggctt tcggcaatta ctccaaaagg gggcgctctc ttttattgat
    34561 cttacgcatc ctgtatgatg caagcagact aaccctatca acgttggtat tatttcccgc
    34621 agacatgacc cttttagcac tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga
    34681 acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc
    34741 gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt
    34801 tgaagagcag gataacctgc aagaggcgtt aatccgctgg ctttgcgatt atcacaatct
    34861 taatgaagaa gatctgcgta aaagcctcta ctggcatcag gataacgacg cggttagcca
    34921 tttaatgcgt gttgccagcg gcctggattc actggttctt ggggagccgc agatcctcgg
    34981 tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat atgaaggcca gcgaactgga
    35041 acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg
    35101 tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc
    35161 gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg
    35221 tcatctgcgc gaacataaag tacagaagat gattatcgcc aaccgcactc gcgaacgtgc
    35281 ccaaattctg gcagatgaag ttggcgcgga agtgattgcc ctgagtgaga tcgacgaacg
    35341 tctgcgcgaa gccgatatca tcatcagttc caccgccagc ccgttaccga ttatcgggaa
    35401 aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa ccaatgctgt tggtggatat
    35461 tgccgttccg cgcgatgttg agccggaagt tggcaaactg gcgaatgctt atctttatag
    35521 tgtggacgat ctgcaaagca taatttcgca caacctggcg cagcgtaaag ccgcagcggt
    35581 tgaggcggaa actattgtcg ctcaggaaac cagcgaattt atggcttggc tgcgagcaca
    35641 aagcgccagc gaaaccattc gcgagtatcg cagccaggca gagcacgttc gcgatgagtt
    35701 aaccgccaaa gcgttagcgg cccttgagca gggcggcgac gcgcaagcca ttatgcagga
    35761 tctggcatgg aaactgacta accgcttgat ccatgcgcca acgaaatcac ttcaacaggc
    35821 cgcccgtgac ggggataacg aacgcctgaa tattctgcgc gacagcctcg ggctggagta
    35881 gcagtacatc attttctttt tttacagggt gcatttacgc ctatgaagcc ttctatcgtt
    35941 gccaaactgg aagccctgca tgaacgccat gaagaagttc aggcgttgct gggtgacgcg
    36001 caaactatcg ccgaccagga acgttttcgc gcattatcac gcgaatatgc gcagttaagt
    36061 gatgtttcgc gctgttttac cgactggcaa caggttcagg aagatatcga aaccgcacag
    36121 atgatgctcg atgatcctga aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa
    36181 gaaaaaagcg agcaactgga acagcaatta caggttctgt tactgccaaa agatcctgat
    36241 gacgaacgta acgccttcct cgaagtccga gccggaaccg gcggcgacga agcggcgctg
    36301 ttcgcaggcg atctgttccg tatgtacagc cgttacgccg aagcccgccg ctggcgggta
    36361 gaaatcatga gcgccagcga gggtgaacat ggtggttata aagagatcat cgccaaaatt
    36421 agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatctg gcggtcatcg cgtgcagcgt
    36481 gttcctgcta cggaatcgca gggtcgtatt catacttctg cttgtaccgt tgcggtaatg
    36541 ccagaactgc ctgacgcaga actgccggac atcaacccag cagatttacg cattgatact
    36601 ttccgctcgt caggggcggg tggtcagcac gttaacacca ccgattcggc aattcgtatt
    36661 actcacttgc cgaccgggat tgttgttgaa tgtcaggatg aacgttcaca acataaaaac
    36721 aaagcgaagg cgctttcagt gctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc
    36781 caacaggccg aagcgtctac ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt
    36841 aaccgtactt acaatttccc gcaggggcgc gttaccgatc accgcatcaa cctgacgctc
    36901 taccgcctgg atgaagtgat ggaaggtaag ctggatatgc tgattgaacc gattatccag
    36961 gaacatcagg ccgaccaact ggcggcgttg tccgagcagg aataatggaa tatcaacact
    37021 ggttacgtga agcaataagc caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa
    37081 tcctgctgga gcatgttacc ggcagagggc gtacttttat tctcgccttt ggtgaaacgc
    37141 agctgactga cgaacaatgt cagcaacttg atgcgctact gacacgtcgt cgcgatggtg
    37201 aacccattgc tcatttaacc ggggtgcgag aattctggtc gttgccgtta tttgtttcgc
    37261 cagcgacctt aattccgcgc ccggatacgg agtgtctggt ggagcaggca ctggcgcggt
    37321 tgcctgaaca accttgccgt attcttgatc tcgggacggg taccggggcg attgcgcttg
    37381 cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg
    37441 tctccctggc acaacgtaat gcccagcatc tggcgatcaa aaatatccac attctgcaaa
    37501 gcgactggtt tagcgcgcta gccgggcagc agtttgcgat gattgtcagc aatccgccgt
    37561 atattgacga gcaggaccca catcttcaac aaggcgatgt ccgctttgag ccgctcactg
    37621 cgctggttgc ggcagacagt ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta
    37681 acgcgctggt atccggcggc tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg
    37741 tgcgacaagc atttatcctc gcggggtatc atgacgtcga aacctgccgt gactatggtg
    37801 ataacgagcg cgtaacgctc ggccgctatt atcaatgaca agtttttcta cactgcttag
    37861 tgttcatctt attagtctcg cgctttctgt tgggctatta accttacgtt tctggctacg
    37921 ttatcagaag catccacagg catttgctcg ctggacgcgc attgtgccgc cggttgtcga
    37981 tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa gcgcacatcc tgccattttc
    38041 cgggcaggca cagtggctga ctgaaaagct gtttggagtt atcatttata tcgttttggg
    38101 ttttattgca ctcgattatc gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc
    38161 gctggcgttg gtggtgctgt acatcatcat taaactcgcc accacaaaag taccgttact
    38221 ggggtaagtc atgagatcgt tagctgattt cgaatttaat aaagcgccat tgtgcgaagg
    38281 catgatcctg gcttgcgaag caatccgccg cgattttccc tcgcaagatg tttacgacga
    38341 actggagcgt ctcgttagtc tggcgaagga agaaatcagc cagcttctgc ctttagaaga
    38401 gcagctggaa aaactgatcg cgctgtttta cggcgactgg ggatttaaag cctcacgcgg
    38461 cgtttatcgt ctttccgatg cattatggct ggaccaggtg ttaaagaatc gacagggcag
    38521 tgcggtatca ttaggtgcgg ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct
    38581 gccggtgatt ttccctacgc agctgatatt gcgcattgaa tgtccggatg gcgaaatttg
    38641 gctgattaat ccttttaacg gtgaatcgtt aagcgaacat atgctggacg tatggttaaa
    38701 gggaaatatc agcccgtcgg cggaactgtt ttatgaagac cttgatgaag ctgataacat
    38761 tgaggtaatc cgcaaattgc tggatacact caaagcctcg ttgatggaag aaaatcagat
    38821 ggagctggcg ttacgcacca gcgaagcttt attacaattc aaccctgaag atccctatga
    38881 aattcgcgat cgcgggttga tttatgcgca actggattgc gaacacgttg cgttgaacga
    38941 tttaagttat ttcgttgaac agtgtccgga agacccgatc agcgaaatga tccgtgcgca
    39001 aataaataac atcgcgcata aacatattgt gctgcattaa ttaatcgaca ttttactcaa
    39061 gattaaggcg atcctatgaa acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat
    39121 gacctgccgt tcgtactgtt tggcggtatg aacgtgctgg aatctcgcga tctggcgatg
    39181 cgcatttgcg agcactacgt aactgtgacc cagaaactgg gtatccctta cgtgttcaaa
    39241 gcctcttttg acaaagccaa ccgctcctcc atccactctt atcgtggacc gggcctggaa
    39301 gaagggatga aaatcttcca ggagttgaag cagacttttg gcgtgaaaat tatcaccgac
    39361 gttcacgaac caagtcaggc acagcccgtt gctgatgtcg tggatgtgat tcagttgccg
    39421 gcgtttcttg ctcgccagac tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt
    39481 aacgtcaaga aaccacagtt tgtcagcccg ggacagatgg gtaatatcgt tgataaattc
    39541 aaagaaggcg gcaacgaaaa agtgattctt tgcgatcgtg gtgctaactt cggctatgac
    39601 aacctggttg tcgatatgct gggcttcagc attatgaaga aagtgtctgg taactcgccg
    39661 gtgattttcg acgtgaccca cgcactgcaa tgccgcgatc cgtttggcgc agcttccggt
    39721 ggtcgtcgtg ctcaggtggc tgagctggca cgagccggta tggcggtagg tctggcgggg
    39781 ctgtttattg aagcgcatcc ggatccggaa catgcgaaat gtgatggtcc atccgcgctg
    39841 ccgctggcta aactggaacc gttcctcaag cagatgaaag cgattgatga tctggtgaaa
    39901 ggtttcgaag aactggatac cagcaagtaa tcttttttgc ttgaaaaata aagtcttagc
    39961 gttctgcgtt aagacttttt tcatgggtgc cagatacaaa aa
//
LOCUS       DAFATA010000029        39943 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFATA010000029 DAFATA010000000
VERSION     DAFATA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.029"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            403..1692
                     /gene="sdaC"
                     /locus_tag="LGJ20_001975"
     CDS             403..1692
                     /gene="sdaC"
                     /locus_tag="LGJ20_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBK2386043.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1750..3117
                     /gene="sdaB"
                     /locus_tag="LGJ20_001976"
     CDS             1750..3117
                     /gene="sdaB"
                     /locus_tag="LGJ20_001976"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBK2386044.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3229..3984
                     /gene="xni"
                     /locus_tag="LGJ20_001977"
     CDS             3229..3984
                     /gene="xni"
                     /locus_tag="LGJ20_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBK2386045.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4039..5187)
                     /gene="fucO"
                     /locus_tag="LGJ20_001978"
     CDS             complement(4039..5187)
                     /gene="fucO"
                     /locus_tag="LGJ20_001978"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBK2386046.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5215..5862)
                     /gene="fucA"
                     /locus_tag="LGJ20_001979"
     CDS             complement(5215..5862)
                     /gene="fucA"
                     /locus_tag="LGJ20_001979"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBK2386047.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6409..7725
                     /gene="fucP"
                     /locus_tag="LGJ20_001980"
     CDS             6409..7725
                     /gene="fucP"
                     /locus_tag="LGJ20_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBK2386048.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7758..9533
                     /gene="fucI"
                     /locus_tag="LGJ20_001981"
     CDS             7758..9533
                     /gene="fucI"
                     /locus_tag="LGJ20_001981"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBK2386049.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9642..11060
                     /gene="fucK"
                     /locus_tag="LGJ20_001982"
     CDS             9642..11060
                     /gene="fucK"
                     /locus_tag="LGJ20_001982"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBK2386050.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            11062..11484
                     /gene="fucU"
                     /locus_tag="LGJ20_001983"
     CDS             11062..11484
                     /gene="fucU"
                     /locus_tag="LGJ20_001983"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBK2386051.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11542..12273
                     /gene="fucR"
                     /locus_tag="LGJ20_001984"
     CDS             11542..12273
                     /gene="fucR"
                     /locus_tag="LGJ20_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBK2386052.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12317..13417)
                     /gene="rlmM"
                     /locus_tag="LGJ20_001985"
     CDS             complement(12317..13417)
                     /gene="rlmM"
                     /locus_tag="LGJ20_001985"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBK2386053.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13410..13805)
                     /locus_tag="LGJ20_001986"
     CDS             complement(13410..13805)
                     /locus_tag="LGJ20_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBK2386054.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13824..14741)
                     /gene="gcvA"
                     /locus_tag="LGJ20_001987"
     CDS             complement(13824..14741)
                     /gene="gcvA"
                     /locus_tag="LGJ20_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBK2386055.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15092..15319)
                     /locus_tag="LGJ20_001988"
     CDS             complement(15092..15319)
                     /locus_tag="LGJ20_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBK2386056.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15511..16716
                     /gene="csdA"
                     /locus_tag="LGJ20_001989"
     CDS             15511..16716
                     /gene="csdA"
                     /locus_tag="LGJ20_001989"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBK2386057.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16716..17159
                     /gene="csdE"
                     /locus_tag="LGJ20_001990"
     CDS             16716..17159
                     /gene="csdE"
                     /locus_tag="LGJ20_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBK2386058.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(17210..18016)
                     /gene="tcdA"
                     /locus_tag="LGJ20_001991"
     CDS             complement(17210..18016)
                     /gene="tcdA"
                     /locus_tag="LGJ20_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBK2386059.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18093..19190)
                     /gene="mltA"
                     /locus_tag="LGJ20_001992"
     CDS             complement(18093..19190)
                     /gene="mltA"
                     /locus_tag="LGJ20_001992"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBK2386060.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19399..19475
                     /locus_tag="LGJ20_001993"
     tRNA            19399..19475
                     /locus_tag="LGJ20_001993"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19433..19435,aa:Met,seq:cat)
     gene            19509..19585
                     /locus_tag="LGJ20_001994"
     tRNA            19509..19585
                     /locus_tag="LGJ20_001994"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19543..19545,aa:Met,seq:cat)
     gene            19619..19695
                     /locus_tag="LGJ20_001995"
     tRNA            19619..19695
                     /locus_tag="LGJ20_001995"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19653..19655,aa:Met,seq:cat)
     gene            complement(19770..21023)
                     /gene="amiC"
                     /locus_tag="LGJ20_001996"
     CDS             complement(19770..21023)
                     /gene="amiC"
                     /locus_tag="LGJ20_001996"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBK2386061.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21255..22586
                     /gene="argA"
                     /locus_tag="LGJ20_001997"
     CDS             21255..22586
                     /gene="argA"
                     /locus_tag="LGJ20_001997"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBK2386062.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22648..24474)
                     /gene="recD"
                     /locus_tag="LGJ20_001998"
     CDS             complement(22648..24474)
                     /gene="recD"
                     /locus_tag="LGJ20_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBK2386063.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24474..28016)
                     /gene="recB"
                     /locus_tag="LGJ20_001999"
     CDS             complement(24474..28016)
                     /gene="recB"
                     /locus_tag="LGJ20_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBK2386064.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28009..30897)
                     /gene="ptrA"
                     /locus_tag="LGJ20_002000"
     CDS             complement(28009..30897)
                     /gene="ptrA"
                     /locus_tag="LGJ20_002000"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBK2386065.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPHKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31073..34441)
                     /gene="recC"
                     /locus_tag="LGJ20_002001"
     CDS             complement(31073..34441)
                     /gene="recC"
                     /locus_tag="LGJ20_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBK2386066.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPTTGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34454..34777)
                     /locus_tag="LGJ20_002002"
     CDS             complement(34454..34777)
                     /locus_tag="LGJ20_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK2386067.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34762..35169)
                     /locus_tag="LGJ20_002003"
     CDS             complement(34762..35169)
                     /locus_tag="LGJ20_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBK2386068.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(35166..35729)
                     /locus_tag="LGJ20_002004"
     CDS             complement(35166..35729)
                     /locus_tag="LGJ20_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBK2386069.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35720..36190)
                     /locus_tag="LGJ20_002005"
     CDS             complement(35720..36190)
                     /locus_tag="LGJ20_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBK2386070.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(36375..37169)
                     /gene="thyA"
                     /locus_tag="LGJ20_002006"
     CDS             complement(36375..37169)
                     /gene="thyA"
                     /locus_tag="LGJ20_002006"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK2386071.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37176..38051)
                     /gene="lgt"
                     /locus_tag="LGJ20_002007"
     CDS             complement(37176..38051)
                     /gene="lgt"
                     /locus_tag="LGJ20_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK2386072.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38202..>39943)
                     /gene="ptsP"
                     /locus_tag="LGJ20_002008"
     CDS             complement(38202..>39943)
                     /gene="ptsP"
                     /locus_tag="LGJ20_002008"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK2386073.1"
                     /translation="RIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLT
                     GALEEAANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWA
                     VKTVIEKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADE
                     LSATTLAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVD
                     GYRGELLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSP
                     EHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVG
                     ADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTS
                     LDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGT
                     NDLTQYILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMC
                     VAILIGLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFM
                     ERRGMGGLIRGGL"
BASE COUNT         9588 a        10873 c         9960 g         9522 t
ORIGIN      
        1 aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat tatcgccatg
       61 attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc cccggaagaa
      121 gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt ttgatttaca
      181 gagtaaagcg ttgggataat ctatcttcca agtagattat tgtatttgag atcaagatca
      241 ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg cggtaaatga
      301 ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc cgggctgaac
      361 tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac gactcaaacc
      421 agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac catgtggatg
      481 ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt
      541 gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat gacgtttttt
      601 gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga agacatcacc
      661 gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct gctctatttc
      721 ttcgctatct acccgatcct gctggtttat agcgtagcaa tcaccaatac agttgaaagc
      781 ttcatgtctc accagctggg tatgacacca ccgccgcgtg cgattctgtc gctgatcctg
      841 atcgtgggta tgatgaccat cgttcgcttc ggtgagcaga tgatcgttaa agcgatgagt
      901 attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct gatcccgcag
      961 tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac cgggaacggt
     1021 ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa ccactctccg
     1081 atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa
     1141 tgctcgaaga tccttgcatt cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc
     1201 ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga gcagaacatc
     1261 tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg gatggctccg
     1321 attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg cgcacgtgaa
     1381 ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat cgaaatcaac
     1441 aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt tgccaccctg
     1501 aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc gatgatcctg
     1561 ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac
     1621 atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat cttctactct
     1681 ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat
     1741 gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc ttccagttct
     1801 cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat tgcccgtaat
     1861 ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc tctgaccggt
     1921 aaaggccacc acactgatat cgccattatt atgggcctgg cgggtaacct gccggatacc
     1981 gtggatatcg attccatccc tggttttatt caggatgtga atactcatgg tcgcctgatg
     2041 ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa cttccatgca
     2101 gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg cgataaagtc
     2161 gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga agagcatttt
     2221 ggccagcaga atagcgcacc ggttgaagtt ccttatccgt acagttcagc agccgatctg
     2281 caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa aaatgagctg
     2341 gcgctgcaca gcaaagaaga gctggaacag cacctggcga acgtatggga agtcatgcgt
     2401 ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca
     2461 cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac cactgacccg
     2521 atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga gaacgctgct
     2581 ggcggacgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc ggcagtgctg
     2641 gcgtactacg acaagtttat ccgcgaagtg aacgctaact cactggctcg ttacctgctg
     2701 gtagccagtg ccattggttc tctttataag atgaacgcgt caatttctgg tgctgaagtc
     2761 ggctgccagg gtgaagttgg tgtggcgtgc tcaatggcgg cggctggtct ggcagaactg
     2821 ttaggtgcaa gcccggcgca ggtgtgcatc gcggcggaaa tcgccatgga gcacaacctc
     2881 ggtctgacgt gtgacccggt cgccggacag gtccaggtgc catgcatcga gcgtaacgcc
     2941 attgcggcag taaaagcggt gaacgccgca cgtatggcgc tacgccgtac cagcgagccg
     3001 cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc
     3061 aagtaccgcg aaacctctcg cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg
     3121 ctctccagag gcctcgtttt gcgaggcctc ttcccgattt ctcatccagc cgtagcctgt
     3181 tccggcatcg aatgttaccc ttatcgcctg atctttaagg gggttatcgt ggctgttcat
     3241 ttgcttattg tcgatgcact gaatcttatt cgtcgcattc atgccgttca gggatcgccc
     3301 tgtgtcgaaa cctgccagca tgcgctcgat cagctcatta tgcacagcca gccaacccac
     3361 gcggtcgccg tttttgatga tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca
     3421 gattacaaag cgggtcgacc gccaatgccg gaagagttgc acgacgagat gcctgcatta
     3481 cgcgccgcct ttgagcaacg cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat
     3541 gacttagccg ccacgctggc ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt
     3601 tcgacagata aaggctactg tcagttactt tcaccgacat tacgtattcg tgattacttc
     3661 cagaaacgtt ggctggatgc gccatttatc gataaagaat ttggcgttca accgcagcag
     3721 ttgcccgatt actggggact tgcggggatc agcagttcaa aggtaccggg tgttgcggga
     3781 atcggaccaa aaagcgccac gcagctgctg gtcgagtttc agagtctgga agggatatat
     3841 gagaatctgg atgcggttgc cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg
     3901 gcgtttctgt gccgcgatat tgcccgctta caaaccgatt tgcatatcga cggcaattta
     3961 cagcaattgc ggttggtacg gtaacggcga gccggatacg ccgcaaacgt cgtatccggc
     4021 attatcacat cagcgcattt accaggcggt atggtaaagc tctacaatat cctcaagcgt
     4081 tgcttcacgc gggttgccac cggtacaaac atcatccagt gccgcctgcg ccagtgccgg
     4141 aatgtcttcc ttgcgtaccc caacatcacg caaatgtggc ggaataccga catcacggtt
     4201 gagagcaaac accgcttcaa cagcggcatt acgcgcctct tccaggctca taccttccac
     4261 tttcacgccc ataacgcgcg cgatatcgcg gtacttctca ccggtaaagt cagcgttata
     4321 gcgcatgaca tgcggtagca ggatggcgtt tgcaacaccg tgtggagtgt tataaaacgc
     4381 gcccagtgga tgcgccatac catgcaccaa ccctaaccca acattcgaga agcccatacc
     4441 cgcaacatac tgcccgagcg ccatttcttc tccggcatcc ttatcaccag caaccgatcc
     4501 tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc agtgcatcgg ttagcgccca
     4561 cgcgccacgg gtaatatacc cctcaatagc atgagtgagc gcatcgacac ccgtcgcagc
     4621 tttcagcgct ggaggcatac catccatcat gtcagcgtca ataaacgcca cctgcgggat
     4681 atcatgcgga tcaacgcaaa caaacttgcg ccgtttttct tcgtcagtga tcacgtagtt
     4741 aatggtcact tctgccgcag tgcctgctgt ggtggggatt gccagaatcg gtacactggg
     4801 tttattggtc ggggaaagtc cttccaggct acgcacatcg gcaaactccg ggttgttgct
     4861 gataatgcca atcgctttac aagtatcctg tggagaacca ccaccaatag cgatcaggta
     4921 atccgcgccg ctattctgga atacaccgag cccttctttg acgacagtaa ttgttgggtt
     4981 gggcactacg ccgtcgtaaa tcgcccatgc cagccctgca gcatccatct tatcggtcac
     5041 tttcgccacc acgccgcatt gcaccagcgt tttatcggtg acgatcagcg ccttctgata
     5101 accacggcgt ttcacctcat cggttaaagc cccaacagca ccccgaccaa accatgccgt
     5161 ttcgttcaga atcattctgt tagccatcat ccttctcctt gttgctttac gaaattactc
     5221 ttcaattcgt aacccatagg ttttgaattt ctccagcact acggcaatct cttcatcgct
     5281 cagcactggc accgggtccg taatcgccag ggtcgtcagg taaagttgcg ccagcacttc
     5341 aacttcatgc gccagccata acgctttttc cagattcacc tcacaagcga taagcccatg
     5401 atgttgtaac aaagttgcct tacgattttt gagagcctgc gcaacatgtt cagaaagttc
     5461 acgtgttcca aaggtcgcat aaggcgcgca aggaatagaa ttaccgccag ccgccgcaat
     5521 catgtagtga atagcgggga tcgatcggtt aagaatggaa actgccgtgc aatgaacggc
     5581 atgattgtga acaaccgcgt tggcatccgg tctgctttga taggccgcca tatggaaacg
     5641 ccattcgctt gaggggagct ttccttcctc atgtttaccg ttgccatcaa taaagacaat
     5701 atgcgactcc gtcagttttt catatggaat gcctgtaggc gtaatcagca tcccatcctg
     5761 ataacgtaca ctgacgttcc ccgctgtccc ctggttcagt cccaggcggg tcatttccag
     5821 gcaagtgtca ataatctgac gagcaagttt atttcgttcc attagctacc tctctctgat
     5881 tcaaaacagg gcaataatgt tgttcctttc acactattga attagccgtt taattaccca
     5941 ccatcttctt cctgattaac cagaaagaaa ttcacaagct tatattttgt gacctggttc
     6001 aactaatcac agtaaataac tgcaagttct ctttttataa ccccattaaa aatgaccgct
     6061 cttaaaaata tttatcaaaa cggtcatttt tctattcact caagccagga atgcccgttt
     6121 tcggcataaa caattaatac ggtcatctgg tttgtgtttt ttatgattta ttttctgaaa
     6181 cgggcatgaa atttcgatta ttaaagtgat ggtagtcaca taaagtcacc ttccagctaa
     6241 taagtgtgac cgccgtcata ttacagagcg ttttttattt gaaaatgaat ccatgagttc
     6301 atttcagaca ggcaaatatt cactgatatg aagcccgaac tcgctggttt tgcacttttg
     6361 aaaacataac cgattacgtg cttaagcttc tgaacctaag aggatgctat gggaaacaca
     6421 tcaatacaaa cgcagagtta ccgtgcggta gataaagatg cagggcaaag cagaagttac
     6481 attattccat tcgcgctgct gtgctcactg ttttttcttt gggcggtagc caataacctt
     6541 aacgacattt tattacctca attccagcag gcttttacgc tgacaaattt ccaggctggc
     6601 ctgatccaat cggcctttta ctttggttat ttcattatcc caatccctgc tgggatattg
     6661 atgaaaaaac tcagttataa agcagggatt attaccggat tatttttata tgccttgggc
     6721 gctgcattat tctggcccgc tgcagaaata atgaactaca ccttattttt agttggccta
     6781 tttattattg cagccggatt aggttgtctg gaaactgccg caaacccttt tgttacggta
     6841 ttagggccgg aaagcagtgg tcacttccgc ttaaatcttg cgcaaacatt taactcgttt
     6901 ggcgcaatta tcgcggttgt ctttgggcaa agtcttattt tgtctaacgt gccacatcaa
     6961 tcgcaagacg ttctcgataa aatgtctcca gagcaattga gtgcgtataa acacagcctg
     7021 gtattatcgg tacagacacc ttatatgatc atcgtggcta tcgtgttact ggtcgccctg
     7081 ctgatcatgc tgacgaaatt cccggcattg cagagtgata atcacagtga cgccaaacaa
     7141 ggatcgttct ccgcatcgct ttctcgtctg gcgcgtattc gtcactggcg ctgggcggta
     7201 ttagcgcaat tctgctacgt tggcgcacaa acggcctgct ggagctattt gattcgctac
     7261 gctgtagaag aaattccagg tatgactgca ggctttgccg ctaactattt aaccggaacc
     7321 atggtgtgct tctttattgg tcgtttcacc ggtacctggc tcatcagtcg cttcgcacca
     7381 cacaaagtcc tggccgccta cgcattaatc gctatggcac tgtgcctgat ctcagccttc
     7441 gctggcggtc atgtgggctt aatagccctg actttatgca gcgcctttat gtcgattcag
     7501 tacccaacaa tcttctcgct gggcattaag aatctcggcc aggacaccaa atacggttcg
     7561 tccttcatcg ttatgaccat cattggcggc ggtattgtca ctccggtcat gggttttgtc
     7621 agtgacgcgg cgggcaacat ccccactgct gaactgatcc ccgcactctg cttcgcggtc
     7681 atctttatct ttgcccgttt ccgctctcaa acggcaacta actgaacata ttttccgaat
     7741 aaagtgagga atctgtaatg aaaaaaatca gcttaccgaa aattggtatc cgcccggtta
     7801 ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga acaaacaatg aatatggcga
     7861 aagctacggc cgcactgctg accgagaaac tgcgccatgc ctgcggagct gccgtcgagt
     7921 gtgtcatttc cgatacctgt atcgcgggta tggctgaagc cgctgcttgc gaagaaaaat
     7981 tcagcagtca gaatgtaggc ctcaccatta cggtaacgcc ttgctggtgc tatggcagtg
     8041 aaaccatcga catggatcca acccgcccga aagccatttg gggctttaac ggcactgaac
     8101 gtcccggcgc tgtttacctg gcagcggctc tggcagctca cagccagaaa ggcatcccgg
     8161 cattctccat ttacggtcat gacgttcagg atgccgatga cacatcgatt cctgccgatg
     8221 ttgaagaaaa actgctgcgc tttgcccgcg ccggtttggc cgtcgccagc atgaaaggta
     8281 aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc cggttctatt gttgatcaca
     8341 acttctttga atcctggctg ggaatgaaag tccaggcggt ggatatgacc gaactgcgtc
     8401 gccgtatcga tcagaagatt tacgacgaag ccgaactgga aatggcactg acctgggctg
     8461 ataaaaactt ccgctatggc gaagatgaaa ataacaaaca gtatcaacgc aatgccgagc
     8521 aaagccgcgc agttctgcgc gaaagtttac tgatggcgat gtgtatccgc gacatgatgc
     8581 aaggcaacag caaactggcc gatattggtc gcgtggaaga atcacttggc tacaacgcca
     8641 tcgctgcggg cttccagggg caacgtcact ggaccgatca atatcccaat ggtgacaccg
     8701 ccgaagcgat ccttaacagt tcatttgact ggaatggcgt gcgcgaaccc tttgtcgtgg
     8761 cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat gggtcaccag ctcaccggca
     8821 ccgctcaggt atttgccgat gtgcgtacct actggtcacc agaagcaatt gagcgtgtaa
     8881 cggggcataa actcgatgga ctggcagaac acggcatcat ccatttgatc aactccggtt
     8941 ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga aggtaacccg acgatgaagc
     9001 cgcactggga aatctctcag caagaggctg acgcttgcct cgccgctacc gaatggtgcc
     9061 cggcgatcca cgaatacttc cgtggcggcg gttactcttc ccgcttcctt accgaaggcg
     9121 gcgtcccgtt caccatgacc cgtgtcaaca tcatcaaagg cctgggaccg gtactgcaaa
     9181 tcgcggaagg ctggagcgtg gaattgccga aggatgtgca tgacatcctc aacaaacgca
     9241 ccaactcaac ctggccaacc acctggtttg caccgcgcct caccggtaaa gggccgttta
     9301 cggatgtgta ctcggtaatg gcgaactggg gcgctaacca tggggttctg accatcggcc
     9361 acgttggcgc agactttatc actctcgcct ccatgctgcg tatcccggta tgtatgcaca
     9421 acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc tgcgcacggc atggatattg
     9481 aaggccagga ttaccgcgct tgccagaact acggcccgtt gtacaagcgt taatacatta
     9541 ctccctgatg tgatgcccgg tcgctccggc taccgggcct gaacaagcaa gagtggttag
     9601 ccggataagc aatgttatcc ggctatattg caggagcgat tatgaaacaa gaagttatcc
     9661 tggtactcga ctgtggcgcg accaatgtca gggccatcgc ggttaatcgg cagggaaaaa
     9721 ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc gatggaaaac aacacctggc
     9781 accagtggtc tttagacgcc attttgcaac gctttgctga ttgctgtcgg caaatcaata
     9841 gtgaactgac tgaatgccac atccgcggta tcgccgtcac cacctttggt gtggatggcg
     9901 ctctggtaga taagcaaggc aatctgctct atccgatcat tagctggaaa tgtccgcgaa
     9961 cagcagcggt tatggacaat attgaacagt tgatctccgc acagcggttg caggctattt
    10021 ctggcgtcgg agcctttagt ttcaatacgt tatataagtt ggtgtggttg aaagaaaatc
    10081 atccacaact gctggaacgc gcgcacgcct ggctctttat ttcgtcgctg attaaccacc
    10141 gtttaaccgg cgaattcact actgatatca cgatggccgg aaccagccag atgctggata
    10201 tccagcaacg cgatttcagt ccgcaaattt tacaagccac cggtattcca cgccgactct
    10261 tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct acagaacagc gccgcagcaa
    10321 tgctcggctt acccgttggc ataccggtga tttccgcagg tcacgatacc cagttcgccc
    10381 tttttggcgc tggtgccgac caaaatgaac ccgtgctctc ttccggtaca tgggaaattt
    10441 taatggtccg tagcgcccag gttgatactt cgctgttaag tcagtacgcc ggttccacct
    10501 gcgaactgga tagccaggca gggttgtata acccaggtat gcaatggctg gcatccggcg
    10561 tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac accctggcaa atgttgattg
    10621 aagaagctcg tctgatcgcg cctggcgcgg atggcgtaaa aatgcagtgt gatttattgt
    10681 cgtgtcagaa cgctggctgg caaggagtga cgcttaatac cacgcggggg catttctatc
    10741 gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa tctacagatg ctggaaaaaa
    10801 tcgggcactt taaggcctct gaattattgt tagtcggtgg aggaagtcgc aacacattgt
    10861 ggaatcagat taaagccaat atgcttgata ttccggtaaa agttctcgac gatgccgaaa
    10921 cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt aggggaattc aacagcccgg
    10981 aagaagcccg tgcacagatt cattatcagt accgttattt ctacccgcaa actgaacctg
    11041 aatttataga ggaagtgtga aatgctgaaa acaatttcgc cgttaatttc tcccgaacta
    11101 ttgaaagtgc tggcagagat gggacatgga gatgaaatta ttttttccga tgctcacttt
    11161 cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg gcctgttggt gagcgacttg
    11221 ctccaggcga ttatcccgtt atttgagctg gacagttatg caccgccgct ggtgatgatg
    11281 gcagcggtag aaggtgacac tctcgatcct gaagtagaac gacgttaccg taatgcgctt
    11341 tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc gttttgcgtt ttatgaacgg
    11401 gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag cgaagtacgg gaatattctt
    11461 ttaaaaaaag gggtaacacc gtaatctcat accggtacgc ccgcatgacg cgggcggtta
    11521 tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc aagcgatagt cgacctgctg
    11581 ctgaaccata ccagcctgac cacggaagct ctctctgaac agctaaaggt cagtaaagaa
    11641 accattcgtc gcgatctcaa tgaattacag acgcagggta aaattctgcg caatcatgga
    11701 cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg atccctttca catccggctg
    11761 aaaagccatt atgcgcataa agcagatatc gcgcgcgagg cgctcgcgtg gattgaagaa
    11821 gggatggtga tagccttaga cgccagttca acttgctggt atctggcacg ccagttgcct
    11881 gacatcaaca ttcaggtctt caccaatagc catccgattt gccatgaact cggtaaacgc
    11941 gaacgcattc aactgatcag ttccggcggc acacttgagc gcaaatatgg ctgttacgtc
    12001 aatccctcgc tgatttccca acttaaatcg ctggaaatcg atctgtttat tttttcttgt
    12061 gaagggatcg atagcagcgg cgcattgtgg gactccaatg ccatcaacgc tgattacaaa
    12121 tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga ttgataaaag taaatttaat
    12181 cgttcagggg aagcccgcat cgggcatctg gatgaggtaa cgcacattat ttctgatgag
    12241 cgccaggttg caacttcttt ggtaacagcc tgacggaaag ggtagcaggc cggagacgac
    12301 gccccggcct tgcctgttat cgctcgtcgc gacgaccacc caccgcagcc cagatgcggc
    12361 ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg ccgcgcctga atctgagcat
    12421 ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag attgtgtgac acttcttcgt
    12481 agcgtttttt catcggcagt ttgaggttga aaatggtttc acggcaccag ccattaacca
    12541 gccactgcgc catcaatgcc gcaactttcg ccggtttttc aaccatatcg cataccatcc
    12601 aggagatatt gctgcgcgtc ggacggaatt tgaaaccgtc ttcccgcagc cacgtcacct
    12661 gtccggtatc catcagactt tgcgccatcg ggccgttgtc gacggaataa acccacatgt
    12721 tgcgcttcac cagttggtag gtccagccgc cagggcaagc gcctaaatcc accgcccaca
    12781 tcccgttcgc caggcgttca tcccactcat ccgcaggaat aaacacatga aatgcctctt
    12841 ccagtttgag cgtggaacga ctcggcgcat ctgccggaaa tttcaggcgc ggaatgccca
    12901 tatagaacgg cgaattattg ttgctgtatg agtaaccggt atagcagcag cctggtgcaa
    12961 taaagaatac atgcacaacc ggacgcttcg gcgtttcata gttcgccagc accccggcat
    13021 cgcgcaaggc agcgcgtagc ggaacagtaa atttacggca gaatttcagt aactctttgc
    13081 tttcgttggt atcggcaact tcaacacgca gttcaccgcc cttctctact acgccctgta
    13141 acatgccgac aatgggggta atacgatctt ctggcggcaa atgctgcagg agttccccca
    13201 ccacaaacca ctggcgggca aaaattaatg aactgaacgg cagctcacgg attaacttat
    13261 cgccatcatc aggttgataa cattcataaa tgacataacc cgcattctct ttcacgcggg
    13321 caaaaccgaa aatttcccgc tggccggctt tatcggtaat ttctgcggcg cactcttttt
    13381 caaagcccgg acggcacagc aatacaacct tattcatgac ttacgccctt gcgctttaaa
    13441 cggatagcac caactaacat taacgcccag cccgcgagga agctcacgcc gccaaccgga
    13501 gtgacaaacg cccacaaacg cagatgggac agcgccaggc aataaaggct gccgctgaac
    13561 aacaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa accagatact gatgcgacgc
    13621 tgcattgcca ccgccagacc taagatcgcc agcgtatgaa acgcctggta ttcgaggccg
    13681 gtctggatcc agcccatctc aacggccccc atggttttac ttaacacatg cgcgccaaaa
    13741 gcgcccagag ccacaaaaat gaagccgcta atggcggcga aaatcagcat gaaacggctg
    13801 gtcatggtcg taccctacgt aaattattgt tcataacgaa agcggaattt ttcttgttca
    13861 gcagcggctt tcgccaggat ccattggcga aaggcggcta ttttacccag ttctgcctgg
    13921 ctgtcatgac aaaccagata aaaagcgttt ttactgacca gaacatcatt aaacgggcaa
    13981 acaagacgtc cggcctcgat ttcagattgc gccatcacgt tatttgccaa cgccactccc
    14041 tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac taaaaattgg cccttgctga
    14101 acgttgatat gatttaaccc caactgtcgg gtatatgtct gccagtcacg gcgtgacgca
    14161 tcatgtaata acgtatgttt agccagatct tccggggtct tcaagggttt ttcgcctgtc
    14221 agcagtagcg gcgaacacac cggcaataaa tattcggcgt acagtttttc cacccgtagc
    14281 cccggccagt tgccccgacc ataaaatatc gccacatcaa catcatccgc cagcttatct
    14341 tcctgacgat caaccgcctg gattcgaacg tcaattcccg gataagctga attaaagctg
    14401 gaaagtcgcg gaaccaacca atgaatggcg aaactgggga gtaaactgac cgtcaacgcc
    14461 cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg ttaattgcga aaatatctct
    14521 ttgatatcga ggaaatagct ttgcccttcc tcggtcagca ggagtgaacg attacggcgg
    14581 cggaacagtt ttagccccaa aaaatcctca agagacttga tttgatgact tactgcggcc
    14641 tgggtcacaa aaagctcttc tgctgcgcga gtgaaactta aatggcgtgc tgcggcatca
    14701 aaaactcgta aggcatttag cggtggtaat cgtttagaca tggcttttaa actttgatgt
    14761 taaatgaatt taacaattag atcacactgt gtaacctatt agttttttta atctgagcca
    14821 ttataaattg tccgttgagc ttctaccagc aaatacctat agtggcggca cttcctgagc
    14881 cggaacgaaa agttttatcg gaatgcgtgt tctggtgaac ttttggctta cggttgtgat
    14941 gttgtgttgt tgtgtttgca attggtctgc gattcagacc atggtagcaa agctaccttt
    15001 tttcacttcc tgtacattta ccctgtctgt ccatagtgat taatgtagca ccgcctaatt
    15061 gcggtgcttt tttttacctt gcgatcgcga attactgatc cagttcgacc atctctttca
    15121 cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc tttatacgaa atcataccgg
    15181 tatcgttatc agtctgtggt ttgccgtcag agacgatggt acgtccgtca ttggtgtgca
    15241 tcacatagtt cgaaccggaa caggcgctca gggcaaaagt cagcatacag gcagaaataa
    15301 ttgcggcagt ctttttcatc atcttctcct taaagcgatt aataaatctt aatagtattg
    15361 attaacaggc taaaattaac gcctaacact attcagcata tgttacttgc gcggcttcgc
    15421 caggatatcc agataattct gatggttagc actctccttg tatcaaagtg aattttgcgt
    15481 cacgatcggt gcatcaagcc gaggagtacc atgaacgttt ttaatcccgc gcagtttcgc
    15541 gcccagtttc ccgcactaca ggatgcgggc gtctatctcg acagcgccgc gaccgcgctt
    15601 aaacctgaag ccgtggttga agccacccaa cagttttaca gtctgagcgc cggaaacgtc
    15661 catcgcagcc agtttgccga agcccaacgc ctgaccgcgc gttatgaagc tgcacgagag
    15721 aaagtggcgc aattactgaa tgcaccggat gataaaacta tcgtctggac gcgcggcacc
    15781 actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc cgcgtctgca accgggcgat
    15841 gagattattg tcagcgtggc agaacaccac gccaacctcg tcccctggct gatggtcgcc
    15901 caacaaactg gagccaaagt ggtgaaattg ccgcttaatg cgcagcgact gccggatgtc
    15961 gatttgttgc cagaactgat tactccccgt agtcggattc tggcgttggg tcagatgtcg
    16021 aacgttactg gcggttgccc ggatctggcg cgagcgatta cctttgctca ttcagccggg
    16081 atggtggtga tggttgatgg tgctcagggg gcagtgcatt tccccgcgga tgttcagcaa
    16141 ctggatattg atttctatgc tttttcaggt cacaaactgt atggcccgac aggtatcggc
    16201 gtgctgtatg gtaaatcaga actgctggag gcgatgtcgc cctggctggg cggcggcaaa
    16261 atggttcacg aagtgagttt tgacggcttc acgactcaat ctgcgccgtg gaaactggaa
    16321 gctggaacgc caaatgtcgc tggtgtcata ggattaagcg cggcgctgga atggctggca
    16381 gattacgata tcaaccaggc cgaaagctgg agccgtagct tagcaacgct ggcggaagat
    16441 gcgctggcga aacgtcccgg ctttcgttca ttccgctgcc aggattccag cctgctggcc
    16501 tttgattttg ctggcgttca tcatagcgat atggtgacgc tgctggcgga gtacggtatt
    16561 gccctgcggg ccgggcagca ttgcgctcag ccgctactgg cagaattagg cgtaaccggc
    16621 acactgcgcg cctcttttgc gccatataat acaaagagtg atgtggatgc gctggtgaat
    16681 gccgttgacc gcgcgctgga attattggtg gattaatgac aaacccgcaa ttcgccggac
    16741 atccgttcgg cacaaccgta accgcagaaa cgttacgcaa taccttcgca ccgttgtcgc
    16801 aatgggaaga taaatatcgc cagttgatca tgctggggaa acagcttccg gctttgccag
    16861 acgagttaaa agcgcaggct aaagagattg ccggatgcga aaaccgcgtc tggctgggat
    16921 ataccgtgac tgaaaacggt aaaatgcact tctttggcga cagcgaaggg cgcattgtgc
    16981 gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa aaccgtcgcc gaattgcagg
    17041 cacagtcgcc gctggcattg tttgatgagc tgggattgcg tgcacagctt agcgcctcac
    17101 gcagccaggg gttgaatgcg ttaagcgagg cgattatcgc tgcggcgaag caggtttaag
    17161 cacagaacca ggtcggataa gacgttcgcg tcgcatccga cctgattgtt taaccctgac
    17221 gcgccgcttt cgccatcatc ttcttcagcg catgagaaac cgcaacaaac ccaaaggtgg
    17281 cggtcaccat cgttgccgcg ccaaatccag atgcacaatc catccgcttc ggcccttctg
    17341 ccgtggcttt catcgcacac acagtaccgt ctgactgcgg gtacaccagc gcttcagtag
    17401 aaaacacgca atcgacgccg agcttacctt tactgttttt cactacgcca aaatcgcttt
    17461 tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat cgttttcgcc agatcggtaa
    17521 cctgaatctg cgtcgggtca atctgcccac ccgcgccacc ggtcgtgacc agcgggattt
    17581 tattgcgccg acaataagca atcagcgccg ctttgggccg tacactatca atggcatcaa
    17641 tcacgtacga ataacccacg ctcatatact gcgctacgtt atccggcgtc acgaaatcat
    17701 ccaccaccgt tacacggcac tccgggttaa tctggcgaat acgctccgcc atgacttccg
    17761 cttttgccag cccgacgtta tcgcgcagag cgtgaatttg ccgattggta ttggtgacgc
    17821 acacatcatc catatcgata agcgtgattg cgccaatccc cgtgcgcgcc agcgcttccg
    17881 ccgcccagga accgacacca ccgataccaa ccacacaaat atgcgcgtca gcaaacagtt
    17941 gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa acgctgacgc catgcatcac
    18001 taattaccac agacatagca cctcagaatt aaaaagggtg aggttatccc tcaccctggc
    18061 gtatccgtaa aaacggtata cagaataaca catcagccgc taaagacgtt acctgcgccc
    18121 ggggcggttt tcagcaccca gacacgtcca tagtggttgt accaacctgc gcgatgtccg
    18181 gcttccggcc cgatcccttg atagatatcg aagtgttggc ctttgattgc gccaccgaca
    18241 tccagcgcca ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc
    18301 agcggcactt ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca
    18361 gaagcacggc caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt
    18421 ttaaagaaaa cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt
    18481 gtttcgcccc aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg
    18541 tcgatcagca ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa
    18601 aagttaagcg gactgccatc accaaagtcg atatacccgc taccctgcac atccataatg
    18661 aagttatcca tcagggagtt gctgtaagcg agaatatatt tatcactcag cgcccctgcg
    18721 tagatctccg cacgagacgg cagacgacca cgttttggcg gcatacggta aatcggatac
    18781 tggaactcgc cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac
    18841 tgcacgttac catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc
    18901 atattgcggg tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc
    18961 tggttgccat acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca
    19021 ccggcgttaa tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta
    19081 aatttcccgt ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc
    19141 attgccacaa ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc
    19201 ttcaggttaa aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc
    19261 agcctctccc tgacgcaaat tttgcacaaa aaataggctt tagtgatttg tttttgttca
    19321 aaatcatgcc aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt
    19381 atagtgcgca tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc
    19441 gaaggtcgtc ggttcaaatc cggcccccgc aaccaattaa aatttgatga agtaaagcag
    19501 tacggtgacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19561 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcaa tacggtgacg
    19621 cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19681 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgctctccca
    19741 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt cgccccatcg
    19801 gcaaaatacg ctttaattcc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    19861 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    19921 ggaatatcag gcgctttcag cacggcaaac ccggcctgtt caacctgatt tttatgcagc
    19981 ttgttgattt taccgagctt attcagcacc gctttaccaa acttaaggct gtcggcaatg
    20041 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20101 acgccaccaa tcaagtccga ggcgttctgg gtctgtgcca gatattttgc cgcagtactg
    20161 gttgcgcctt tggttgagag cgcaaacact gaggaaccgc tcggctggcg actggtaaag
    20221 gcgtcggcat ggatagagac aaacaggtcg gcacgctgtt tctgggcttt tgctacgcgc
    20281 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20341 ttctcgatca gagagcgcag acggcgagct atttgcaata ccacgtcttt ttcgcgcgtt
    20401 ttgtatttcc ccaccgcacc ggagtcttcg ccgccgtggc cagggtcaag cataataaca
    20461 ataggacgat cgcgccctgc tttacccggt tgtggaccac tttgtgctgg cggcacctgc
    20521 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20581 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20641 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatccg tacggtctgc
    20701 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20761 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttcc
    20821 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    20881 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    20941 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21001 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21061 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21121 acggtaaaca ttctttttat attcagggca ttactgataa aaaagtcgct ctcgcataaa
    21181 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttcg aataataatg
    21241 caaaggggtg tgccgtggta aaggaacgta aaaccgagtt ggtcgaggga ttccgccatt
    21301 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21361 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21421 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21481 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21541 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcgatgagtc
    21601 tcaataacac gccgctacag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21661 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21721 ttgatgaaga cgcgatccat cgtcaactgg acagcggtgc aatagtgcta atggggccgg
    21781 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    21841 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    21901 atgacgacgg tgatattgtc tcagaacttt tccctaacga agcgcaagcg cgggtagaag
    21961 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22021 aagcctgccg cagcggcgtg cgtcgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22081 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22141 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22201 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22261 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22321 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22381 cacggggcga ggttctgctg gaacgcatta ccgctcaggc gaagcagagc ggcttaagca
    22441 aattgtttgt gctgaccacg cgcagtattc actggttcca ggaacgtgga tttaccccgg
    22501 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22561 aagtgttgat ggcggattta gggtaagcga tgaaaattgt cggatgcgac atgcgtaaca
    22621 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22681 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22741 cgacagacgg cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22801 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    22861 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    22921 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    22981 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatta ccggtcgacc
    23041 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgctc
    23101 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagttg
    23161 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23221 ataacgtcca taacccgcaa gagcttcctc aagcatcgca atataatctt ctccgctctg
    23281 taaaagccgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23341 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcactgc cgaaacgata
    23401 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cagttcctgc
    23461 cggaacgtga gttcccgtca ggcggcttag ctgcccggca cgctcggcgg taaagccggc
    23521 attggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23581 acgatcgccg agaaagatca ctcgcgcatg atcgggcaag gcgtcgatca gtctcgacat
    23641 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23701 accggcatga tgacgtaaac gctggctacc cggctgcgca cccagcaatc ggtgcaaagt
    23761 actggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    23821 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    23881 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agtaacttcg ccacggtggt
    23941 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24001 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24061 cgccagtaga gcttcatcaa cctcaatggc atgattcacc tcattgaaga agcgcgctac
    24121 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24181 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24241 tagctcaccg atttcactga cacaggtcgc caacagcgga tgcgacgcct cgttattttc
    24301 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24361 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24421 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24481 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24541 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24601 ataacgccgc caaagtgacg atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24661 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24721 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24781 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    24841 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    24901 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    24961 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25021 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25081 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25141 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25201 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25261 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25321 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25381 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25441 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25501 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25561 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25621 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcgttaaga
    25681 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25741 ttggtgataa acggcagcca gaccaatgga tattccagcc ctttcgattt gtggatcgtg
    25801 acaatctgca ccagatgttt atcactttcg agacgcattt gttggctgga ggcattactg
    25861 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    25921 gttccggctt cttgtagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    25981 cctgccgttg ccagcaagtt ttcagcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26041 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccaca
    26101 tcccacgcat gttcgtcatt gttcagcgtt tcgatatcca gcgcgttcag ccccatcatt
    26161 gacgttgcca gcgcactacg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26221 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26281 acggaaggga tttccagcaa cgttaaggcg tcgcgcacct gggcggcctc ctggcggctg
    26341 cgcaccagca cactgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26401 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26461 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26521 atcgcaggct gcgtttcacc tttaaacaca aaacgtaacg cctgattttt cccggctgat
    26581 ttcactggaa taaacggtat ttcgcgaaac atgaacgcgt catcagtctg gctgaaaagc
    26641 ttgttcacac tgttcaccat tcctggtgcg gaacgccagt tggtgtctaa agtgtagtgg
    26701 gcgtgaactt cgctacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26761 atggcctgtt tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    26821 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcaat cattgccacc
    26881 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    26941 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27001 actgtttcgc ggatctcagc caatgcgcgg gttatcacca gatcacggat cgacaatggt
    27061 tctgcaagca gttgttcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggct
    27121 ttcgtgcgat cttctaagaa acgctgagag aatttttcca gcgactccgg taactgataa
    27181 ctgttggtct cttcttctgc ccaggcgctg atcttctcga tccatttagc ctgattgcta
    27241 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27301 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27361 gccagcgttt catcatcggg cggcggtgct ttgataaccg gtgcttcgcc ttgcagataa
    27421 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac caccagggca
    27481 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27541 agcagagact catcttcaat cagctgctgc tcaaacagca taccggattc aaaggcattc
    27601 aggttgagca tgcgctggca aaaaccgtga atagtaaaga ctgccgcttc atccatctgc
    27661 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27721 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27781 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    27841 agttcttcaa cggtcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    27901 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    27961 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28021 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28081 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acaggatagc
    28141 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28201 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28261 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28321 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28381 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28441 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28501 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28561 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28621 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28681 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28741 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28801 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    28861 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    28921 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    28981 ggacttcgcc tgctcaagct gatcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29041 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaacca taaggccgtt
    29101 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag
    29161 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29221 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggttcgctgg aaaaataacg
    29281 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcata
    29341 tttcttctct gacttaatca gcgagaaatc gtcaggaata taggggttaa gctccggtaa
    29401 ggagagcgca atattggcag ctttttgctg ccagtcagcg aaagtttgtt cgctgatttt
    29461 atcgacctga tacggcgcat cgacaaaata agccgtttta ttgtgcggct ctttcgggct
    29521 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttctt ttactgcttt
    29581 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaaccg gaacgcgaat
    29641 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29701 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29761 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaggccttt
    29821 atcggttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    29881 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcggaaa gtgtacctgg
    29941 gctgcgatta ccaatcagat aggtaatcaa ttcatccgtt ttactacgga acttcgctga
    30001 gttgttatcg atgcgaaact caacgcgcag aactttatgc ggcagtgccg ggacgtaatg
    30061 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30121 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30181 tttattgctg taaataaccg ccttcatcag attggcggaa tagtacttct cgtggaaatc
    30241 tttcagcgcc tgctgcaccg gattaccagg tttatcgctt aaagtttcga ggttaccacc
    30301 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30361 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc accgcattac gctcacgttc
    30421 ggcatatttc ttgtcgagca aaggttcagc aatagcatcg gccaggcggt ctaccgcacc
    30481 aggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30541 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30601 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30661 atcttccagc gacccaacgg gcaccaccag cgccgagagc gatttaactg cctgcggatc
    30721 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30781 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    30841 gggtgcccaa agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    30901 cggacctcat aatcaacttt attttctggc cagattcaac aggttaacgc aaatcatctg
    30961 ccagaattta atcttgtgct gcacgagtca gcctatgtct atatatccat cagtccgtga
    31021 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacagc actcatgact
    31081 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31141 tctctggtgt taattgccgc cagagccttt gataccagat atcatcacct tcgccacgca
    31201 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31261 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31321 tttcaggtaa caccagcaat ggcgcggaca tcccttcacg atacccctca atcagttgtg
    31381 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc
    31441 gtagaaaaag gcgactttca ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31501 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31561 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31621 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31681 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31741 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31801 tgatttgata acggctaagc ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    31861 cttcagtacg gaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    31921 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    31981 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32041 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32101 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32161 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32221 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32281 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32341 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32401 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32461 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgcgcca
    32521 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32581 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32641 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32701 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32761 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    32821 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    32881 gtccggtggt ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    32941 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33001 ccgccagcac cggcacatcc agtaatgcca gcacgtcttc cgacacaaag cggctgtctg
    33061 gcaacgataa caggctgata aacgcttcca gtaccggatg cgactgccgc gcccgacggt
    33121 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacaca gcctgaataa
    33181 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33241 ggtcttcttc cagcatcgcc agcaggcgat cgtgcaaaac ttcaacttca cgctgcgggc
    33301 tatggcaaac gtggaaggtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33361 tacgggaaaa ctcttcgatg ttcacaccag caacggcacg gttttccagt tccagaatgt
    33421 cagactgaat gttatgcagc aggttatctg gcgtgacatc gacaaaggcg tccagctcct
    33481 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagctta ccccatgaag
    33541 ccagcagcgg gttgccgaca tcctgttcac catcgctgtt aaagagctgc ccggcatttt
    33601 cgctatcacg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgggtca
    33661 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33721 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33781 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    33841 tcgcggactc cagcgtttcg ataaagcgct gatagagatt ggcgcggtgc caacgcggtt
    33901 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggcgcttgc caggcctgtg
    33961 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccac tccgggcgat
    34021 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34081 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcga tccagcaatt
    34141 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34201 ggatctccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34261 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34321 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34381 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34441 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34501 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34561 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34621 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattaat gttcgctgga
    34681 tacccgataa cgcagtgaca atcatcacca tcagcaccat cgcaaccatt acctccggca
    34741 ggctaaagcc ttgttgattc ttcagggaag ctgacataac gccctctctt tcagcggaca
    34801 aaaatcgctc cagccgcgtg gcgaaaaaac aatgtttcca tcaatgactt cgcctgttcg
    34861 ccacaacgaa acgccttcat aaccggcaac caataaggct tcattatctg ccagtaaacg
    34921 caaacaaacc cgggcatcgg ttccagcgta ctgcgagcac tgaactgcgg gctgcgtctg
    34981 ccaggagtgc attttccccc acgccagcgc cgactgaacg atggcctggc ggcgcaatga
    35041 ctgactttcc atgctcacgc gagaggcaaa actgcgatcc tgctgactca ttccttgcaa
    35101 tagcaagcta cccaaaacca gcagcatcag aaccagagcc agtgacgaaa cacctttttc
    35161 gcggttcaca ggttgaatcc tgtcacgcta tagctggcat ccaccacggt ttgcggttca
    35221 gacttactgg cagcacgcat attaaccgtc aacaccggcg agaagccgct gacatcctga
    35281 cgtacgacct gaaaagtgtc gataatgatg gcatccggat tagtgacttt atcccagccc
    35341 ttaccttcac aggatgtcgc accgcgtagc gtttccagca catgctcctt cagacgaaat
    35401 ccaatctggt cagactcttt taccggttcg cgatcccaga taccgttact gttcgcatcc
    35461 cactgcacaa tgatacagtc accctgtccg acaatttcca gcccttcgcc ggtacagctg
    35521 ccatgacaat aacccgccct ctggagatgc ttcgcgacgg taaatacccg cagccagatt
    35581 tcatcttcca gcgccagctt acgggtgctc gttaaacttt cacgctgtaa cgcaggcaga
    35641 aagcgtgcag cccccagcaa caatacgcta ctgatcgcca tagcaatcaa cacttccagc
    35701 agagaaaaac cttgctcttt tacaggcatc cttctgtttc tccttgctga caaagccgga
    35761 gtcttcccca cggcgaaacc accagccacc actcgcccgt tgagtttttg aagcgaatat
    35821 gcccggccca tgcggtattg cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca
    35881 tttcgacttc gggccagcgt ggcacaaaga ccaatggtga actgccatga caggtattgg
    35941 ccccagcagc ggaactcaca aggcaccata acgtcccctc cctgataacg ctgatactgt
    36001 ggtcgcggtt atgccagttg gcatcttcac gtaaatagag caaatagtcc cgcgcctggc
    36061 tggcggtttg ccatagccgt tgcgactgct gccagtattg ccagccatag agtccacttg
    36121 cgcttagcat gaccaaaatc agcatcgcga ccagcgtttc aatcagcgta taaccacgtt
    36181 gtgttttcat gccggcagta tggagcgagg agaaaaaaag acgagggcca gtttctattt
    36241 cttcggcgca tcttccggac tatttacgcc gttgcaggac gttgtaaaat ttcgggaagg
    36301 cgtctcgaag aatttaacgg agggtaaaaa aaaccgacgc acactggcgt cggctctggc
    36361 aggatgtttc gtaattagat agccaccggc gctttaatgc ccggatgtgg atcgtagcct
    36421 tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg tttgataatc
    36481 aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc catatgattg
    36541 ctgtacaggt gcgtgtcgcc ccccgtccag acaaaatcgc ccacttccag atcacactgc
    36601 tgcgccatca tatgcaccag taacgcgtag ctggcaatgt taaacggcag gccgaggaaa
    36661 acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac atagaactgg
    36721 aagaacgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa
    36781 acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg
    36841 tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata cactggtccg
    36901 aggtcgccgt tttcatcggc ccattcgtcc cagatggtga cattgttttc gtgtagataa
    36961 gcaatgttag tgtcgccctg cagaaaccac agcagttcgt ggatgatgga acgcaggtgg
    37021 caacgtttag ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat ctgatgacca
    37081 aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc gtcgagcact
    37141 ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg
    37201 cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat ggaaagaatt
    37261 tgccccatgc tgatgtactg cacccaggca ccggtaaact gcgcgtcggg ctggcggaaa
    37321 aactcaacaa tgatgcgaaa cgcgccgtag ccaatcagga acaagccaga gacagctccc
    37381 attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac accttccagc
    37441 agcagctcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat
    37501 tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc tgggaacagc
    37561 atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat aaagttgccc
    37621 agacgcccgg caccaagacc aaacggaatg agtggtgcga taaaatcaga aacctggaag
    37681 aaggaacgtt tagtacggcg ggcaaagata atcatcacca cgataacgcc aatcaggccg
    37741 ccgtggaaag acatgccgcc gtcccagaca cggaacagat acagcggatc ggccataaac
    37801 tgcgggaaat tgtagaacag aacataacca atacgtcccc cgaggaagac gccgaggaag
    37861 cccgcataga gtaagttttc aacttcattt ttggtccagc cgctgcccgg acgattcgcc
    37921 cgtcgtgttg ccagccacat tgcaaaaatg aaacccacca gatacatcag gccgtaccag
    37981 tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa atgcagatag
    38041 ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac agcgattgaa
    38101 atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag gggacctggc
    38161 tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc tccgcgaatc
    38221 agcccgccca tgccgcgacg ctccataaag gctgcaacct gatggcgaac ttcggtcgcc
    38281 agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcataatc aatgcgccgc
    38341 agcaggtatt ttacccgcgc tacagaacgt ccgttcatag acagatggcg atacccaagc
    38401 ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa acggagatcg
    38461 attccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat tgcaggatga
    38521 agactgtcat aaatgttcgc cacccgggta ttgttgcgat caacggccag aatgtattga
    38581 gtcagatcgt tggtgccaac agagatgaaa tcgacccgct ttgccagatg cggcagcata
    38641 aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg
    38701 atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc atcaacttca
    38761 tcgaggcttg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc attagcacgc
    38821 agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat gcgaatccca
    38881 cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg cttatctgct
    38941 ccgacatcca gcgtacgcaa ggtgacgggt ttatcattga acatttgcag catcccctga
    39001 tactgcgcca cctgttcttc ttccgacggg aaaccacttt gcagcatgaa tgggatttca
    39061 gtgcgataaa gaccgatgcc atcaatacgg ctgcccagtt tttcttcatg ttccgggctt
    39121 aaaccagcat tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc gggtaaattg
    39181 acgtcatctt ccgccagacg gctaagctca atctcttcac taattagccg ctgatattct
    39241 tgcagcagta ccggctccgg atcgaccagc aattcaccgc gatagccatc aacgatcagc
    39301 gtcctacgat gcagcaccga aggctgaata tccgcgccca tcacggtagg gatccccagc
    39361 gcacgtacca tgatcgcagc atgggagttg gctgcgccat ctcgcacgac aacaccgact
    39421 aagcgatcct ggggcagctc agcaagagtt gtcgctgaca gttcatctgc caccagaatg
    39481 aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa cagcaatcgc
    39541 tgacccagcg cacgtaaatc gccagcccgc tctttgagat agttatcgct tagcgcggca
    39601 aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac cgagccttta
    39661 tcaacctcgg caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg cgagtaaaga
    39721 tcgaaaatag ccgccgtttc tttttgtgca ccggcggcaa agcgtttgct gtagcggcga
    39781 aactcgtttg ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag agccggatcc
    39841 agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg ccagccttcg
    39901 gcaatcgcca caccaggtgc tgccggtaat gcgcggattc gcg
//
LOCUS       DAFATA010000030        39935 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFATA010000030 DAFATA010000000
VERSION     DAFATA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.030"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..248)
                     /locus_tag="LGJ20_002009"
     CDS             complement(<1..248)
                     /locus_tag="LGJ20_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="septal ring protein, suppressor of prc, minor
                     lipoprotein; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA"
                     /protein_id="HBK2386074.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLA"
     gene            complement(259..1371)
                     /gene="mrdB"
                     /locus_tag="LGJ20_002010"
                     /gene_synonym="rodA"
     CDS             complement(259..1371)
                     /gene="mrdB"
                     /locus_tag="LGJ20_002010"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBK2386075.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            complement(1374..3275)
                     /gene="mrdA"
                     /locus_tag="LGJ20_002011"
     CDS             complement(1374..3275)
                     /gene="mrdA"
                     /locus_tag="LGJ20_002011"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBK2386076.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            complement(3306..3773)
                     /gene="rlmH"
                     /locus_tag="LGJ20_002012"
     CDS             complement(3306..3773)
                     /gene="rlmH"
                     /locus_tag="LGJ20_002012"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK2386077.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            complement(3777..4094)
                     /gene="rsfS"
                     /locus_tag="LGJ20_002013"
                     /gene_synonym="rsfA"
     CDS             complement(3777..4094)
                     /gene="rsfS"
                     /locus_tag="LGJ20_002013"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK2386078.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            complement(4354..4965)
                     /locus_tag="LGJ20_002014"
     CDS             complement(4354..4965)
                     /locus_tag="LGJ20_002014"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBK2386079.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            complement(4989..5630)
                     /gene="nadD"
                     /locus_tag="LGJ20_002015"
     CDS             complement(4989..5630)
                     /gene="nadD"
                     /locus_tag="LGJ20_002015"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK2386080.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            complement(5632..6663)
                     /gene="holA"
                     /locus_tag="LGJ20_002016"
     CDS             complement(5632..6663)
                     /gene="holA"
                     /locus_tag="LGJ20_002016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK2386081.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            complement(6663..7244)
                     /gene="lptE"
                     /locus_tag="LGJ20_002017"
     CDS             complement(6663..7244)
                     /gene="lptE"
                     /locus_tag="LGJ20_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBK2386082.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAAAIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            complement(7259..9841)
                     /gene="leuS"
                     /locus_tag="LGJ20_002018"
     CDS             complement(7259..9841)
                     /gene="leuS"
                     /locus_tag="LGJ20_002018"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK2386083.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            10076..10558
                     /locus_tag="LGJ20_002019"
     CDS             10076..10558
                     /locus_tag="LGJ20_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBK2386084.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            complement(10628..11605)
                     /locus_tag="LGJ20_002020"
     CDS             complement(10628..11605)
                     /locus_tag="LGJ20_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBK2386085.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLSAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            11769..12476
                     /locus_tag="LGJ20_002021"
     CDS             11769..12476
                     /locus_tag="LGJ20_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBK2386086.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            12473..13900
                     /locus_tag="LGJ20_002022"
     CDS             12473..13900
                     /locus_tag="LGJ20_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBK2386087.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(13910..14464)
                     /locus_tag="LGJ20_002023"
     CDS             complement(13910..14464)
                     /locus_tag="LGJ20_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBK2386088.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            14565..15272
                     /locus_tag="LGJ20_002024"
     CDS             14565..15272
                     /locus_tag="LGJ20_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBK2386089.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15269..16720
                     /gene="djlC"
                     /locus_tag="LGJ20_002025"
     CDS             15269..16720
                     /gene="djlC"
                     /locus_tag="LGJ20_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBK2386090.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(16780..18450)
                     /gene="hscC"
                     /locus_tag="LGJ20_002026"
     CDS             complement(16780..18450)
                     /gene="hscC"
                     /locus_tag="LGJ20_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBK2386091.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(18534..19469)
                     /gene="rihA"
                     /locus_tag="LGJ20_002027"
     CDS             complement(18534..19469)
                     /gene="rihA"
                     /locus_tag="LGJ20_002027"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBK2386092.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(19587..20312)
                     /gene="gltL"
                     /locus_tag="LGJ20_002028"
     CDS             complement(19587..20312)
                     /gene="gltL"
                     /locus_tag="LGJ20_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBK2386093.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(20312..20986)
                     /gene="gltK"
                     /locus_tag="LGJ20_002029"
     CDS             complement(20312..20986)
                     /gene="gltK"
                     /locus_tag="LGJ20_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBK2386094.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(20986..21726)
                     /gene="gltJ"
                     /locus_tag="LGJ20_002030"
     CDS             complement(20986..21726)
                     /gene="gltJ"
                     /locus_tag="LGJ20_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBK2386095.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(21896..22804)
                     /gene="gltI"
                     /locus_tag="LGJ20_002031"
     CDS             complement(21896..22804)
                     /gene="gltI"
                     /locus_tag="LGJ20_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBK2386096.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            23259..25136
                     /locus_tag="LGJ20_002032"
     CDS             23259..25136
                     /locus_tag="LGJ20_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK2386097.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLFSSVFLHSSFSHLLMNMFALLVVGAVAERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAALATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGAMGWQRARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQIDESVLQVRQSLREEFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(25214..26752)
                     /gene="lnt"
                     /locus_tag="LGJ20_002033"
     CDS             complement(25214..26752)
                     /gene="lnt"
                     /locus_tag="LGJ20_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBK2386098.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(26777..27655)
                     /gene="corC"
                     /locus_tag="LGJ20_002034"
     CDS             complement(26777..27655)
                     /gene="corC"
                     /locus_tag="LGJ20_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBK2386099.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(27745..28212)
                     /gene="ybeY"
                     /locus_tag="LGJ20_002035"
     CDS             complement(27745..28212)
                     /gene="ybeY"
                     /locus_tag="LGJ20_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK2386100.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(28209..29249)
                     /locus_tag="LGJ20_002036"
     CDS             complement(28209..29249)
                     /locus_tag="LGJ20_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK2386101.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(29402..30826)
                     /gene="miaB"
                     /locus_tag="LGJ20_002037"
     CDS             complement(29402..30826)
                     /gene="miaB"
                     /locus_tag="LGJ20_002037"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBK2386102.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            30972..32147
                     /gene="ubiF"
                     /locus_tag="LGJ20_002038"
     CDS             30972..32147
                     /gene="ubiF"
                     /locus_tag="LGJ20_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBK2386103.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(32301..32375)
                     /locus_tag="LGJ20_002039"
     tRNA            complement(32301..32375)
                     /locus_tag="LGJ20_002039"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32341..32343),aa:Gln,seq:ctg)
     gene            complement(32413..32487)
                     /locus_tag="LGJ20_002040"
     tRNA            complement(32413..32487)
                     /locus_tag="LGJ20_002040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32453..32455),aa:Gln,seq:ctg)
     gene            complement(32536..32612)
                     /locus_tag="LGJ20_002041"
     tRNA            complement(32536..32612)
                     /locus_tag="LGJ20_002041"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32576..32578),aa:Met,seq:cat)
     gene            complement(32628..32702)
                     /locus_tag="LGJ20_002042"
     tRNA            complement(32628..32702)
                     /locus_tag="LGJ20_002042"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32668..32670),aa:Gln,seq:ttg)
     gene            complement(32737..32811)
                     /locus_tag="LGJ20_002043"
     tRNA            complement(32737..32811)
                     /locus_tag="LGJ20_002043"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32777..32779),aa:Gln,seq:ttg)
     gene            complement(32835..32919)
                     /locus_tag="LGJ20_002044"
     tRNA            complement(32835..32919)
                     /locus_tag="LGJ20_002044"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32883..32885),aa:Leu,seq:tag)
     gene            complement(32928..33004)
                     /locus_tag="LGJ20_002045"
     tRNA            complement(32928..33004)
                     /locus_tag="LGJ20_002045"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32968..32970),aa:Met,seq:cat)
     gene            complement(33385..35049)
                     /gene="asnB"
                     /locus_tag="LGJ20_002046"
     CDS             complement(33385..35049)
                     /gene="asnB"
                     /locus_tag="LGJ20_002046"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBK2386104.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(35306..36058)
                     /gene="nagD"
                     /locus_tag="LGJ20_002047"
     CDS             complement(35306..36058)
                     /gene="nagD"
                     /locus_tag="LGJ20_002047"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBK2386105.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(36106..37326)
                     /gene="nagC"
                     /locus_tag="LGJ20_002048"
                     /gene_synonym="nagR"
     CDS             complement(36106..37326)
                     /gene="nagC"
                     /locus_tag="LGJ20_002048"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBK2386106.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(37335..38483)
                     /gene="nagA"
                     /locus_tag="LGJ20_002049"
     CDS             complement(37335..38483)
                     /gene="nagA"
                     /locus_tag="LGJ20_002049"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK2386107.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            complement(38543..39343)
                     /gene="nagB"
                     /locus_tag="LGJ20_002050"
     CDS             complement(38543..39343)
                     /gene="nagB"
                     /locus_tag="LGJ20_002050"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK2386108.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            39676..>39935
                     /locus_tag="LGJ20_002051"
     CDS             39676..>39935
                     /locus_tag="LGJ20_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK2386109.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKA"
BASE COUNT         9677 a        10118 c        10152 g         9988 t
ORIGIN      
        1 gccagtcccg cctggctaaa tcgagacgga tcctgcacga ttttgtagct tttaccgtcg
       61 cgctggtaat cctgatttgc cgtcgcgttc agtggttcga aacgcgggtc cgccccgcta
      121 atttcaacta tggggccgtt acataccgca ggctgcggta cacttaccgt ctgttgctga
      181 ccatcatcgc ttgtacatgc cgcgagcatt cctgccgcga tgcagatccc gagccactgc
      241 ttacgcattg cgcacctctt acacgctttt cgacaacatt ttcctgtggg tgtggattga
      301 cattacaatc ccgaacccag ccatcagcac aattagcgct gatcctccat aactgaccag
      361 tgggagcgga acccctacaa ccggcagaat accgcttacc ataccaatat ttacgaagac
      421 ataaacgaat aatatcagca ttaagccgcc agccatgacg cgaccaaagg tggtttgcgc
      481 tctggcggct atccacagcc cgcgcatgat cagcagaatg tagagagcga gcagaatcag
      541 aatgcccact aatcccagct cttccgccag taccgcgaag ataaagtcgg tatggcgttc
      601 gggtagaaat tcaagttgtg actgagtgcc atgcagccag cctttgccgc gtaatccgcc
      661 ggagccaata gcaattttag actgaataat gtgatagccc gcgccgagtg ggtctgattc
      721 cgggtccagg agcatcatta cgcgctggcg ctggtaatca tgcatcagga agaaccacag
      781 aatcggaatg aacgccgcca ccagcactac tgcgacgcca atcagacgcc agctaaggcc
      841 agagaggaac agtacaaaca gaccggaaag cgcaacgagg attgatgttc ccaggtcagg
      901 ctgtgcagcc accagcagcg tgggcataaa tatcagcacc agcgcgatgc cagtgttctt
      961 caacgatggc gggcaaacgt cgcggttgat aaagcgcgca accatcagtg gtacggctat
     1021 tttggcaatt tccgacggct gaaaacgaac aataccgagg tccagccagc gttgagcacc
     1081 tttagagatg gcaccgaaag catctaccgc caccagcaaa ataatacaga tgatatagag
     1141 atagggggcc cagccttcat aaacgcgtgg aggaatttgc gccatcacca ccatgatgac
     1201 cagacccatc gcgatttggc cgattttacg ctccatcatg ccaatatcct gaccgctggc
     1261 gctccagata accagggcgc tgtaaaccag caatgccagt aagatcagca gcattgtggg
     1321 atcgagatgg actttatccc agaatgtttt tttattcgga ttatccgtca tgattaatgg
     1381 tcctccgctg cggcaaccgc tggattttcc gcaggcagat cggtgttgtt atcacccagc
     1441 ataatgtggt cgaggatctg gcgcatcagt gtcccaaccg ccggacccgc accaccgttc
     1501 tccagaatca tggcgacagc cacttgcgga ttgttgtatg gcgcaaaggc ggtcatcagt
     1561 ttgtggtcac gtaaacgctc ggcaattttg tgcgcattat aggtttcgtt cgctttcaga
     1621 ccgaagacct gagcggtacc ggatttcgcc gcaattttgt acggtgcgct ggcaaagtat
     1681 ttatgcgccg taccgttagg gcggttagca acaccgtaca taccgtcttt cgccagctcc
     1741 cagtaaccgg aatgaatatc gccgacgggc ggttcatgcg gctgtaccca tggcacctgt
     1801 ttgccgtctt cggcggtgct catcagcaaa tgaggaacct tcacgatacc gtcattaatc
     1861 aggatcatca gtgccttact catctggatt ggggtcgctg tccagtaacc ctgaccgata
     1921 ccaaccggaa tggtgtcacc ctgataccac ggttttttaa agcgtttctg tttccattcg
     1981 cgggtaggca tgttgccgga acgttcttcc gccaggtcga taccggtgta atgaccataa
     2041 ccgaatttac ccatccattc ggagaggcga tcgatcccca tatcgtaggc cacctgatag
     2101 aagaaggtat ccgcagattc ttccagcgat cttgtgacat tcagacgccc gtggccccat
     2161 tttttccagt cacgataacg tttttccgaa cctggcagtt gccaccagcc tgggtcaaac
     2221 agcgtcgtat tgcgcgtgat caccccggcg ctcaatgccg aaaccgccac atagggttta
     2281 actgtagacg cgggaggata aaccccctgt gtggcgcggt tcaccagcgg tgtattcggg
     2341 tcgttcaaca aggcggaata atctttgctg gagataccgt caacaaacag gtttgggtca
     2401 taactaggcg tggaaaccag cgccagcacc ccacctgtac gcggatcggt gacaaccaca
     2461 gctgcgcggc tacccgccag cagcgtttca atatattgct ggagtttgag atccagcgtc
     2521 aggtaaatat cgtgtccggc ttgcggtggt acttctttta actggcggat aacacgccca
     2581 cggttgttaa cttcaacctc ttcataaccg gtctgaccgt gcagcacgtc ttcatagtaa
     2641 cgctcaatgc ccagcttacc gatatcatgc gttgccgcat agttggccag tttgccgtca
     2701 ttattcaggc gttcgacgtc tttatcgttg attttcgaca catagccgat gacgtgggtc
     2761 aacgccgaac cgtaaggata gtaacgacgt ttatagcctt taacttcgac acccggaaaa
     2821 cggtactgat tgacggcaaa gcgagctact tgtacttcgg tcaggttagt tttcaccgga
     2881 atagaggtga aacggtgtga acgtgcgcgc tcttttcgga atgcagcaat atcgtcatcg
     2941 gtcagatcaa ccacgctgcg caaagcgtcc agcgtttgct gcacgttatc gactttctct
     3001 ggcatcattt ctatctggta gatagtgcgg ttgagggcca gagggatacc attacggtcg
     3061 tagataatgc cgcggctggg cgcgataggc accagcttaa tgcggttttc attagagcga
     3121 gtctggtagt cggtaaagcg aacaatttgc agattataca ggttggcgat aagcacgccg
     3181 gtcagcagca aaatccccaa aaaggcgacc agcgcccggc gcacaaacag cgcggactct
     3241 gccgtatagt cgcgaaaaga gttctgtagt ttcatccgct gcgttttcta ctcaaagctc
     3301 ccttatcact cacggtgata aggatggttg gtggtgatgc tccacgcccg gtacagactc
     3361 tctgcgacca gcacgcgaac cagcggatgg gggagggtaa gcgccgacag cgaccagctc
     3421 tgctccgccg ccgctttaca ggcaggcgac aacccttcag gcccgccaat cagtagactg
     3481 acgtcgcgac catccagctt ccagcgttcc agctcagcgg ctaactgcgg cgtatcccag
     3541 ggcttgcctg gaatatcgag ggtgacaatg cggtttttgc ctgcggccgc caacatctgc
     3601 tcaccctctt tgtcgagtat gcgcttgatg tccgcattct tgccgcgttt tccggccgga
     3661 atttcaatca gctcgaaggg catatctttc ggaaaacgac gcaggtactc ggtaaaaccg
     3721 gtttgtaccc agtccggcat tttcgttccc acggcgacaa gttgcagctt cacgcattaa
     3781 ctccagagtt tttccagttc atacaggcga cggctctctt cctgcatgac atggacaatc
     3841 acatcgccca aatccacgac aatccagtcg gcgctgtttt caccttctac gccgagcggt
     3901 aacaggcccg ctgcgcgaga ctcctgcaca acgtggtcag caatggacat aacatgacgg
     3961 ctggacgtac ccgtacagat gatcatgcag tcggtgatgc tggatttgcc ttgaacgtct
     4021 aaggcgatga tgtcctgacc tttgaggtca tcaattttgt cgataacaaa atcctggagt
     4081 gctttaccct gcaagttttc cccctgggtg aatcaaatag ataaaaatgg tctgtcagta
     4141 tacctgaacc agaggcgatt tcgggacaat tgtcgccgaa tcggctttcg aaagtgggct
     4201 atcatcccac cccgcgccgc agattgcatc gccatttttg taaaacaatt tctacaaagt
     4261 cgtgtctggc gaaaaaagtc tggctgcgga gaatatcagc ctgcccgggt ctgtcaatgg
     4321 tcgttgcggc ttacccgtaa aaaaacagaa aagtcatgca ttctcgaccc cgatggcacg
     4381 gctattgagg acgcgtagcg tcgcgaattt ttggttgata tcaatggtgc tccaacaccc
     4441 ctggtcaacg cgaaaatgcc acatagcctc ggcaggcatg ccaattaaac gggcgattaa
     4501 cagactcagt acaccctgat ggctgacgac taaaatattc tgatagtgct gaaattcact
     4561 aagccttgcg ataaagcgtt ccacacgttg cgaaaatgcc tgaaatcctt caccgttcgt
     4621 ggggattgca tgctgccagt cattgcacca cgcgctatag ttttcggcat cttcttgcat
     4681 gaggtcgcga tgatgtcgca tctcccagtc gccaaaaaac atttcgttga gttcagggat
     4741 gatttgcacg gggagctggc ggtcactgag aaccagtcgc gcggtatgct gtgcccgttc
     4801 cagttcactg cataaaacca gatcaaagga aacaccatgt agcagcgtat gcagattttg
     4861 cgcttgctca ataccgcgtg cggtcagggg tgtgggcgca tgaccgctgt acagaccatc
     4921 gacattcgct tgcgtttcgc catgacgaat taaccacagt cgcatcatgc cctccgtaac
     4981 gacaggtatc agcgatacaa gccttgttgg ttaatgtaag tcagtaccgg ttccggcaat
     5041 aaatcctcac acgattcacc gttttgcaaa cgttcgcgga tgatggtcgc cgagatgtta
     5101 aaccacggcg tttcagccag ataaatttta ccggcaggct gaaggtgaag atcttccggg
     5161 ttatgtgtca aatgatcttc cagccattgc tggtattgcg gttgcgccat ttcaagtggg
     5221 taacctggac gccgacagac gatcaaatgt gcattgtcga gtatcgtttc gtattcgtac
     5281 caggtcggaa aggtcagcaa tgaatcctga ccaataataa acgccagcgg cacgtccggc
     5341 ccttgttcct gccgccactc tttcagtgtt tgcgcagtgt aagagggggc attgcgcttt
     5401 agctcgcgtt catcaagagt aaataatggc ttgtcggcaa tcgccagttc aagcatgtgt
     5461 ttacgctgca cgctgttcgc ttccggctgg ggacgatgcg gaggaacatt attagggatg
     5521 attgtgaccc gcgtcagacc aatcaaattc gccagcgttt ccacgggttt aagatgacca
     5581 tagtgcaccg gatcaaaggt gccgccaaac agagcctgta aagatttcat atcaaccgtc
     5641 gataaatacg tccgccaggg gtttatggca caacagaaga gataaccctt ccagctctgc
     5701 ccacactgac tgaccgtaat cttgtttgag ggtgagttcc gttcgtgtca ggagttgcac
     5761 ggcctgacgt aactgcggct ggcttaagcg atttaacgcc tcgcccatca tgccccggcg
     5821 gttctgccat acccgatgct tatcaaacaa cgcacgcagt ggcgtatggg cagactggcg
     5881 tttcaggtta accagtaaca acagttcacg ttgtaatgtg cgcaacaaaa taaccggctc
     5941 gctgccttcc agacgcagtt gctgaagaat atgcaacgcg cgcttacttt ttcccatcaa
     6001 caaagcatca acccaatgaa aaggggtgaa atgcgcggca tcattcaccg cctgttcaac
     6061 gcgcggtaat gtcaatttgc cgtctggcca gagcagcgat aaacgctcca gtgcctgagc
     6121 cagcgccagc aggttacctt cataacagta gcagagcacc tgatttgcag cgtcatccag
     6181 ttctaagttg agctgttttg cgcgcgcagc aacccagcgg ggaagctgag cctgctccgg
     6241 tgtctgacag gtcacctgca cgctgcgatt cgcaagagca gtaaaccagg cggcattttc
     6301 ttgcgctttg cttaatttat taccgcggac gatcaacagc aggtcgtcat gcagaagtcc
     6361 ggtgagtgtg agaagttgtt cattgatcgc cgcattcggt ccgttttctg gtaacaacag
     6421 caatagcgtt tgtcgactgg caaacagact catagcctgg cataacgaaa agatcgcatt
     6481 ccagtcagtg ttgggatcaa tggaaaaagt gtggtgttct tcgaatcctt gtgccgcagc
     6541 tacctgacga acagcgtcct ggctttcctg caataacaga ggatcgttac caagtaaaag
     6601 atacgccgcg cgcagccctt cattgagctg cgcgcggagt tgttccgggt acaaccgaat
     6661 catcagttac ccagcgtggt ggagacgcgt gcaggcgttg ccggagtatc cgttgtggtc
     6721 gacgtctgtt cttcgtcgga acgaatagcc gcagcacgga tgcttggcag cttacgaatc
     6781 agctgttcgg cagcacggtc gtacatctct tttacgatca tgtcttgttc gttatctttc
     6841 gctaacgcca tttgcgggtt atcaaagaac gaacggaaga ctttggcact aatcgggtag
     6901 atatcacggc cggggatcaa cacggtcgca ttcaccgtca tgatcatctg atactctgct
     6961 gtttgaccgt tacggaatac cgatgcggta tctttcgcga tgctcacttt acccaaacgc
     7021 agggatggaa cgtccttacg cgtggtttct ttatcaagca actcgacacc attcagacgt
     7081 aactggttac gcaccgcacg gcttaatggc ccgttcggat cgcctgagtc caggatcatg
     7141 accttcatag tggaaggaac ctgcgtggta tcacgcagat gccagccaca cccggcggtg
     7201 attaacaccg ccagagataa caacaatgtt gccagatatc gcacgcttcc tcccgcgctt
     7261 agccaacgac cagattgagg agtttacctg gtacgtaaat cactttacgc acagtaacgc
     7321 catcaagata ttttgctacc agatgttcct ggccagcacg ttcgcgaacc tgttcttccg
     7381 ttgcgtccac cggaacggtg attttggcac ggactttacc gttaacctgc accacgacca
     7441 gcgtggagtc ttccaccatc gctttttcgt cagcaaccgg ccacggcgcg ttgtcgatat
     7501 cgccttcgcc tttcagttcc tgccacagcg tgaagcagat gtgcggggtg aacgggttaa
     7561 gcatacggac aacggccagc agcgcttcct gcatcagagc gcgatcctgc tcgccatcgg
     7621 ttggtgcttt cgccagtttg ttcatcagct ccataatcgc cgcaattgcg gtgttgaagg
     7681 tctgacgacg gccgatatca tcggtcactt tagcgatcgt tttatgcaca tcgcgacgca
     7741 gcgctttctg atcttcagtc agcgcatcaa cgttcagtgc cgcaacatca ccttttgctg
     7801 tgtgctcgta aaccagtttc cagacacgtt tcaggaagcg gttagcccct tccacaccgg
     7861 attcctgcca ttcgagagtc atatcagccg gagaagcaaa catcataaac agacgaacgg
     7921 tgtccgcgcc gtaacgttca accatcacct gcgggtcgat accgttgttc ttcgacttgg
     7981 acattttgct catgccggta taaaccagtt catggcctgc cgcatctttc gctttcacga
     8041 tacggccttt ctcgtcacgt tcaacgatag catcaaccgg ggaaacccag ttacgttcgc
     8101 cgttttcgcc aacatagtag aaggcatctg ccagcaccat accctgacac agcaactgtt
     8161 tcgctggttc gtcagagttc accatgcctg catcacgcat cagtttgtgg aagaagcgga
     8221 agtagagcag gtgcataatg gcgtgttcga taccaccaat gtagatatcc accggcagcc
     8281 agtagttagc cgcttcggaa tccagcatac cttctttgta ctctgggcaa gtgtagcgcg
     8341 catagtacca ggaggactcc ataaaggtgt cgaaagtgtc ggtttcacgc agcgctggca
     8401 taccgttaac ggtagttttt gcccactccg gatctgcttt aatcgggctg gtaatgccgt
     8461 ccatcaccac atcttccggc aggatcaccg gcagctggtc gtccggggtc ggcattacgg
     8521 taccgtcttc cagcgtcacc atcggaatcg gcgcgcccca gtaacgctga cgagaaacac
     8581 cccagtcgcg caggcggtag ttcactttac gctcgccaac gcccatcgca gtcagtttat
     8641 cggcgatggc gttgaaggcc gcttcatggt caagaccgtt gaattcgcca gagttgaaca
     8701 gcacgccttt ttcagtcagg gcttgctgag aaagatctgg ctcagagccg tcagcagcca
     8761 ggataactgg tttgatgttc aggccgtatt tagaggcaaa ctcgtagtcg cgctggtcgt
     8821 gacccggaac cgccataact gcgcccgtgc cgtactccat caatacgaag tttgctgccc
     8881 aaacgggaat ttcttcgccc gttaatgggt gaacagcttt aaagccagta tcgacgcctt
     8941 ttttctccat cgtcgccatt tcagcttcgg caactttggt gttacggcat tcgtcaataa
     9001 aggccgccag ttcaggatta ttttccgccg ctttctgcgc cagcggatga cccgcagcta
     9061 ccgccaggta ggtacaaccc ataaaggtgt ccgggcgggt agtgtaaacg gtcagcgtgt
     9121 tgtcatagtc gttaacgttg aaggtgatct ccacgccttc ggaacgaccg atccagttac
     9181 gctgcatggt tttaacggtg tctggccagt gatccagttt atccagatcg ttgagcagct
     9241 cgtcagcgta agcagtgatt ttgataaacc actgcgggat ctctttacgt tcaactttgg
     9301 tatcgcagcg ccagcagcag ccgtcgataa cttgttcgtt cgccagtacg gtctggtcgt
     9361 tcgggcacca gttgaccgca gaagtcttct tatataccag gcctttttta tacagctcgg
     9421 tgaagaattt ctgttcccaa cggtagtatt ccggcgtaca ggttgccagc tcgcggctcc
     9481 agtcataacc aaagcccagc attttgagct ggtttttcat atacgcgatg ttgtcgtacg
     9541 tccacggtgc cggagcggtg ttgtttttca ccgccgcgcc ttccgcaggc agaccaaacg
     9601 cgtcccagcc gatcggctgc aggacgtttt tgcccagcat acgctggtag cgggcgatca
     9661 cgtcaccgat ggtgtagtta cgtacgtggc ccatgtgtag tcgaccagaa ggatagggaa
     9721 gcatagacag gcagtaatac ttctctttgc tctcgtcttc ggttacttca aatgtgcgct
     9781 tctcatccca atgaagctgt actttggatt ctatctcttc cgggcggtat tgctcttgca
     9841 tggcagccag tggtcctgtt ttcaatacgg ctacaaatgt agcgttgagg tggtttttca
     9901 gatccgcata gcatagccca aacgtccgcg tcaaaacagc ctttcgcgca ctcgacgttg
     9961 aaatgatgcc cggattattc atacattaat ttacagagtt tgtgggcgta ttagcaaagc
    10021 aaggaacaaa gaacgtctat tattatagtc agttaacgac ccgggagatg aaacgatgaa
    10081 caaggttgct caatattacc gtgaactggt cgcgtcactg agcgaacgcc tgcgcaatgg
    10141 cgaacgtgat atcgacgcac tggtggaaca ggcgcgcgag cgcgtaataa aaacagggga
    10201 gttaacgcga accgaggtcg atgagctgac gcgagctgta agacgtgacc tggaagagtt
    10261 cgccatgagc tatgaagaga gcctgaaaga agaatctgac agcgtcttta tgcgggtgat
    10321 taaagaaagc ttatggcagg agctggcaga tatcaccgat aaaacgcagc ttgaatggcg
    10381 cgaagttttc caggacctca atcatcatgg ggtttatcac agcggagaag tggtcgggct
    10441 gggaaatctg gtctgcgaga aatgtcactt ccatctcccg atctacacac cggaagtgct
    10501 gacgctatgc ccgaaatgtg gtcatgacca gttccagaga cgcccgtttg agccgtaata
    10561 gtctgatttc gcacagcaat aaagagaata tgggccgaat gaaattcccc ggcccttgtt
    10621 tttatctcta cagtaaatct tcagtctcac gaatttcctg actgacatct ctggttccgg
    10681 atgactgaac tttacgatac aggtcgagag cctgtcggag atcgacaggt cttccttctc
    10741 cgtagtgata cataatggcc aggttatacc aggcagtggc gtcattgcat tcagcggctt
    10801 tcgtaaacca ttcaaaggca gcctgataat ctttctcaac gccttgtccg tgtttataca
    10861 tataaccgat gttgacgtaa gcgccgacgc tttcctgagc ggcagccttc agataccagt
    10921 acatcgcttg cttatagtcc tggtccaccc cttcgccagc gttataatcc catgcaattt
    10981 gaaactgggc gtgccgatta ccctgctgtg cgcttttcaa ataccagaat gcggcgagtg
    11041 tcttgttttg agcaacgcct tttccgtctt cataaagatc ggccagattg ttttgcgcgt
    11101 cactatggcc ttgtaatgca gcttgtttgt accagaaaaa tgccagcgca tagtcttttg
    11161 cgactccgtt gccatttctg tacatccacc caagattatt ttgcgcataa gacattccgc
    11221 tctccgcggc tatctgatac cagacaaccg cttctttata attgggggct tctccacgat
    11281 ccagtgtcca gccaagggcg ttggaagcga aagtatggcc ttgtagggca gcttttttca
    11341 gccagaagat ggcttgctca ttatctttat gacggctttt atcctcggta tacttttgcc
    11401 ctaaggaata ctgtgcttca caatgacctt gctcagcgga cagcttcagc cagtaaaagg
    11461 ctttttcgtc gtccggagaa tcaattgcgc tatcgcgatt ataataaaac ccaacaatat
    11521 attgagcctc gcaatcgcct ttttcagcac gttcgataat ctcatctatt gataaattat
    11581 cgcagcaact tgacgtgaaa atcattatca tttccttatg gttgcagata aaacatatta
    11641 tatttttatg tgttccattt ttgcgtaagc cttattttat gcgtattatg tcgcgtcaga
    11701 aaagtataaa taaacgagcc tcgcaatgcg gctaatattc atttaatgaa tatttaagga
    11761 taaattatat ggacatggag tcgcaaaaaa tattgtttgc actttccact ccgatggaaa
    11821 tacgcaatga atgctgttta ccttcgcatt catcacccaa aatgtattta ggtacatgct
    11881 tttttgacct ttcttcctcc tggggaattg atgcccgtga tgacctgctg cgcacaattc
    11941 accgaatgat cgataacggt catgccgctc ggttggcagg gttttatcac cgctggtttc
    12001 gttattcgcc atgtgaatgg cgtgactatc ttgctgaact aaatgagcaa ggtcaggctt
    12061 atgcgcaatt tgtcgccagc accgccgaat gctgtggtga gggaggaatt aaagcctggg
    12121 attatgtccg gatgggtttt ttaagccgaa tgggcgtact taataactgg ttgagcgaag
    12181 aagaaagtct atggatccag tcgcgcattc atctacgggc tctacgttat tatcgtaact
    12241 ggcggcaata ctttgccggt tatacctttg gtcggcaata ttggcagtct cccgaagatg
    12301 atcatctgca gttattacgc gaattcttag cgcgtaaaga atacgacgat tccggcaatg
    12361 atatgtttta tcaattattt gccagtgatg atgcgtatta ccctaccttg tcctggcaac
    12421 cattggctta ctattctgca tgcccggaaa cgcttaagga tatgagcgac ctatgaaaaa
    12481 ttgctggaag attctcgaaa tagaggaaac gactgacgtc gatattatcc gccgcgctta
    12541 tctggcgctg ttaccgtcct ttcatccaga aaccgatccg cagggtttta aacaacttcg
    12601 tcaggcgtat gaggaggcgc tacggattgc gcagtcgcct gctaaatctg tttggcaacc
    12661 agaagaatat gaggtagcag aacatgaaat tctgctcgcc tttcgtgcgt tacttgcctc
    12721 tgatagcgaa cgttttctgc cctccgcctg gcagcgattc attcagcaat taaattattg
    12781 ctcgatggaa gagattgatg aattacgctg gtcgctgtgc acaatcgcca tgaacactgc
    12841 ccatttatcc ttcgagtgcg tggtgttatt agcagaaaga ttgcggtggt tgcaggagga
    12901 aaacgtcggg gaaatagacg aagaagaact ggaatccttt ttatatgcca ttgcgaaggg
    12961 gaatgttttt aacttccaga ccattctgca tctgcccgtt gccgtgcaaa atgacaccat
    13021 cgatttttac caaatgttcg ctcggatttg gtcatcgcat ccagaatggc tgacattgta
    13081 tttagcgcaa catcgcgcag tgattatccc cgatgatgca aaactgcaca gaaatttact
    13141 ccgctggtat agcgcaggtc gcctgggaat tcccgaactc ctggattacg cccggtcgtg
    13201 gcgggaagct gaatctgata atgaagatgc gcgttattat gaatacgcac aacgcgtcta
    13261 ttgtggagaa ggcgaaagct tgctggcaga actttgtgac tactggcgcg agtatccctc
    13321 cacccaggcg gatgctttaa tattgcaatg gtgccgtcag catcgggtcg attattaccc
    13381 gttagtggtg atgatgattg aagctcgtga actggttaac gatcagggaa aaccgctact
    13441 ttatatcccc ggcgacagcg cccgtacgcg ttttcattta tacgaaatac tcagcgatga
    13501 aaaactctct gctctggggc gttcactggt cgagatggtt ttgcacaaag gacgtaagcc
    13561 gcggatctca ctcacgcgtg atacagaaca tcccttatgg ccattatatt tagttgccaa
    13621 acaattagtg caggcgaacc aaccaaccga agaatcatta atgccgattg tgagccgcct
    13681 tgatgcagaa gatcgttgtc cactggaagc attaattatt cgtcgattat taattcaggc
    13741 ggcgaatttt accgagaagc aaactgttga accggagccg caaccgcagc caatgcccgt
    13801 tgacgatggt gggccaggct gtctgggcat cattaaaatt attttctata tttttatctt
    13861 tgctggtttg atagggaaaa tactccatct gttcggttga cagtcgacgt taatgtgcgg
    13921 gaagcccggc aagtaatctt tccgcatttt tgtaatgttg tttggcggcc agttttaacc
    13981 atttttgaga ttcataatgg ttaacattaa tacctgagcc atttctgtag atcaaaccta
    14041 aagtatattg agcatgagca tacccttgtt cagctgcttc cttataccag agaattgctt
    14101 tacgggtgtc gggtttgacc cccagacctt tgaggtagat ccaccccagt ttgctttgtg
    14161 cgcgcggatt ttcctgttct gctgattgct cataccagta tctcgcctgt gtataatcca
    14221 gtggtttacc gtcgctacca tgaagataaa attctccaag gcgatattgc gacgttgagt
    14281 gaccatgaat cgcggaaagc tccgtggccg ccgtggtaaa cggtagactg cccggtgagc
    14341 ttaaataatc aaaggcataa ttcaccatgg ctttatcacg caacgttgtt ttcttgggtt
    14401 ggctaataca ggtaattgct gcaactataa atattgcgaa tatatacatt aattttttat
    14461 tcatatgtga atgcttcgat tttcttttta ttttgaaagc gtcaagttta tactcatcaa
    14521 taataaaaag tagtcagtta atgcacttaa gggattatta acaaatgaat aaggaagaac
    14581 aatatcttct ttttgcgctt tctgcgccca tggaaatact taatcagggt tgcaaacccg
    14641 cgcatgactc gccaaaaatg tataccggga taaaagaatt cgatctctca tcatcctggg
    14701 gaataaataa tcgtgatgat ttaattcaga ctatttatca gatgactgat gatggtcatg
    14761 ccaatgattt agctggctta tatctgacat ggcatcgctc ttcccctgaa gagtggaaag
    14821 cattaattgc tggtggctcg gagagagggt tgatttatac ccaattcgtt gcccaaaccg
    14881 ctatgtgctg tggagaaggg ggaatcaaag cctgggatta tgttcgaatg gggtttctgt
    14941 cacgggtcgg tgtgctcaac aactggctga cggaagaaga gagtttatgg ctgcaatcgt
    15001 gcgtctatgt acgagctcac cattactatc acagctggat gcactatttt tccgcctatt
    15061 cgttaggacg tctctactgg caatcttctc agtgcgaaga caacacttcg ctacgcgagg
    15121 cgctaaccct gtacaaatac gatagcgccg ggagtcggat gttcgaagag ctggcagcgg
    15181 gaagcgatcg tttctatgcc acgcttccct ggcagccatt aactgtccag cctgaatgcc
    15241 cggtaacgct taaggatgtg agcgacctat gaaaacatgt tggcaaatcc tcgaaattga
    15301 aagcacgacg caaatagaca ttatccgcca ggcttatctt gctcgcttac cgttatgtca
    15361 tcccgaaacc gatccgcagg ggtttaaagc attacgccag gcctatgaag aggcgctgcg
    15421 actggcggta aatcctgtcg aggaagcaga tgatgaagaa aaagatgccg ccgctgaaca
    15481 tgaaatactg cgtgcattca ggacattact ggattcagaa agtgatcgtt ttcagccctc
    15541 cgcctggcag aaatttattc agcaattaaa tacctggaac atggaggatg tcgatcaatt
    15601 acgctggccg ctgtgtgcaa tcgctataga agcgcgatat ctttcattaa attgtgcttc
    15661 tttgctggca gagcgtttga actggcattc gtttaatgac agcgaaggaa tggatgagga
    15721 agaaagggag gcttttcttg aggccattca ggctggtgat tgtttcgatt tccttagcct
    15781 tctggaatat cccgttgcgt tgcagaacca gactgttgag tattacttcg cgctggaacg
    15841 ttgctgccgt taccatcctg actacgtcac tgcgtttttg gcgatggaag gtccgtggtt
    15901 cattcctgat gatgcaaagt tacatcgcaa actgttgcgc tggtacagct cggtgcaaac
    15961 aggtatggcg gaactcattc ctgtagccaa acagtggcaa atggaagaac cagaaagcga
    16021 agatgcccga tattacttgt gtgctcaaag gttgtactgc ggcgaggggg aaagcctgct
    16081 tgccgatctc tgcgcgtacc gggaaagtta cccatctaca caagctgata atttgttgtt
    16141 gcagtggagc aagcgtcatt gcccggatta tttcgcgtta ttagtgatgg ttatcgaagc
    16201 gcggagcatg gtagatgcgc aaggtcaacc gctgaaatat gttcctggtg agagcgcccg
    16261 gacgcggctg ttatgggcgg aaattttaca tagcggaaaa ttatcgccgt taggtcaatc
    16321 gtttattgag tcgttattct tcaaacgcaa agcatgggcg tggtggaaat cgagagtcgg
    16381 tagcgagaca gaagaagatt caccgttact ggatttgtat cgggtagcgg aacaggtagt
    16441 acttgaagcg tttccgaaac aagagatgct ggcccgtctt aatacaaggc tggaaggcgg
    16501 agatgctcat ccattagagg ccattatcac ccggttgctg ttgacgaaag taaaactcga
    16561 gccggaggat gaagatgtcg atgagccaac acctgaaaac catgaagaaa aaaatgatga
    16621 gggtgaaaaa ccacagagca ttaccagcat tatcaaaatc agtttaacgg tgctggttat
    16681 aggttatgct ctcggcaaaa tcgcgatgtt gtttagttga cgatgtgttt tttgcgccga
    16741 tgccggatgc agcatttacg ccgcatccgg cagccgtgat tatgaggtct caatggctaa
    16801 ataaacgctc gcccgccgcc tgacctcatc gacgcgggcc atatcgtttg actgcagcgc
    16861 cgcatcaaaa tcggtgatca cccggccaat ctcttctcgc tcgtcaccca gcgcccgcgc
    16921 ccacaactct tccagtttgg ctttaaaggt gcgattgatg agcatatcgc gcggataaat
    16981 tttcaatgcg gataaccgcg tccgactctc ttcaatctgc tgcgcgctca atgttaccgg
    17041 gctgtggtta atcactctgg acttaacgct gccgtcttcc agaagcacgt caacttcaag
    17101 caacccgttg atatcataac taaagcgaat atcaatcgac tgataagccc cggttttctt
    17161 caacggcaca tcgaaggatt ccaccagaat gttgttttta actttgtggt tttctccctg
    17221 atagacgtta accgtaattg aatcctgttc cgggtgcatg gttgaataag tttctacacg
    17281 cgacacgggc acagtggtgt ttcgttcaat aatcggcgag aaaatgccgg aaacgccctg
    17341 gcggttaact tcaacgccca acgagtaagg gcaaatatca gtgaggatta cctcttcaat
    17401 atcttcactg cgtaagcggc aggcggcctg gattgctgcg cccagcgcga caatggtgct
    17461 cggatcgtaa ctttgatacg gtaatttgcc aaacagacgc acggcgattc gctgcaccag
    17521 cggcatttgt gacgcgccgc caaccagcac cagactgtcg atttgactcg gcttcagacg
    17581 tgcatcgcgc aacgcctgtt caatcggcac tcgcaagcga ttgagcagcg gcaaccacaa
    17641 atcttccagt tcgttctcgt aaaattcgca ctcccgcgtt tcatcctggt actgccagcg
    17701 aatgtgcaat ggcgattgat tgctacattt tgccgcttcc acacaggcgt acaaggctcc
    17761 cagttcactc tcgttaagcg tggtcctggc gacatccgcg cgttttaaaa cctcatcgac
    17821 cagcatatgg gtaaaatctt cgccaccaag aaagttgtcg ccagcggagg cgtgaacttc
    17881 aatcaccggc gtggcgtact caagcaccgt aacgtcaaac gtgccgccac cgagatcaaa
    17941 caccagcgaa cgggtatttt gttgggtatg caggccatac gccatcgcag ctgctgtggg
    18001 ttcattaatt aagcgtaccg catttaaccc ggctaactcc gctgctaaac gggtatgctt
    18061 gcgttgttca tcgctgaaat aagccggaac ggagatcacc acatctttaa tcggacgttg
    18121 cagaaattct tcggcatctt cttttaatga gcgtaatacc aaagaggaca gttctggcgc
    18181 gttaaatgtg tcgctgccta accgccagtt ggtattactg cccattgcac gtttaaataa
    18241 cgctgccgtt ttatccggat gcgaagtacg ccgtgataca gccggttttc cgactaaaat
    18301 atgattattt tcatccatgc taattatgga tggtgttaaa tattcaccga acttatttgg
    18361 aattaattgc gcggcaccgt ctttccagac ggcaattaaa ctattggtag tgccgagatc
    18421 gataccaatg gcgagttctg cattatccat tgcatttata tcttgaaaga aaaagagata
    18481 aacagattaa gaccacccag gtaacgcgtg gtgatcttaa tcaatgacgt gtgttaagcg
    18541 taaaatttca gacgatcagc cagtaaatca acaaagccct gacgatcaac atcgaccatt
    18601 acagtggcat tcggtttatt gccagtcaga taataataat caacaaccgt cataccctgg
    18661 ctatatttcc cctgtgtttc cacgccaacc cagcgctcaa cagaggtaaa caactcgggt
    18721 ttcaacagcc aggcgatggt gcatgggtca tgcagcggtg caccgacaaa gccccatttt
    18781 tcgtctttat gatattcgag gaagaaatcc agcagttcgg caacaatggt tgaaacaggg
    18841 ttgccaatcg cgcggaaacg ctcggtgtct tcaacgtgga tttgtgcttt gtgagtaaca
    18901 tccagaccgg ccatcaccac tgggatccct gactggaaga caatttctgc cgcttccggg
    18961 tcaacgtaaa tattaaattc agccgcaggc gtccagttac caagccccat tgcgccgccc
    19021 ataatcacga tacgggcaat tttgctatgc agttccggat ggctattgag cagcaaggca
    19081 acgttagttt gcggtccggt agacacaatc gtgacaggtt ccgcactttc gcgcagcgtt
    19141 ttcgccatca gctctaccgc cgtacagttt tgcggtgcga acgtcggttc cggtaatgcc
    19201 gggccatcca gaccgctttc gccgtgcaca ttgtccgcga taatcaactc acgcattaac
    19261 ggttttaccg cgccgcctgc taccggaata tcggtgcgat taagcaaggt cagcatacga
    19321 agaacattgc gtaaggtttt ttctggtgtc tggtttccgg cggaagacgt aattgctttg
    19381 acatcaagct ctggtgaggc gagggcgaga actattgcga tagcgtcgtc atgacctggg
    19441 tcgcaatcta acagaattgg cagtgccatt gttgctcctt gttgtgtgct tctttgcgac
    19501 aagggtaacg ccaggatgta acagatacga ggggcgaaac gataaagcgt gagatggcgc
    19561 gcacttgggt atgcgcgcca gagtgattaa tgcaggattt tcgcgaggaa gtctttcgcg
    19621 cggtccgatt tcggatcatc gaagaaagcg tctttcggcg agtcttcgac aattttaccc
    19681 tcgtccataa agatcacccg attcgccact ttacgggcaa aacccatttc gtgggtcacc
    19741 accatcatgg tcattccttc gtttgccagt tccaccatca cgtccagcac ttcgttgatc
    19801 atctccggat ccagcgccga tgtcggttcg tcgaacagca tcgcaatagg atccatacac
    19861 aacgcgcgag cgattgccac acgctgctgc tgaccgccgg aaagctgcgc cggaaattta
    19921 ttggcatgtg cggaaagtcc cacgcgctcc agcaatttca atgctttttc acgagcgggc
    19981 gctttatcgc gtttaagcac tttcacctgc gccagggtca ggttttcgat aatcgacaga
    20041 tgagggaaca gctcgaaatg ctggaatacc atcccgacgc gggaacgcag ctttgccaga
    20101 tcggttttct tgtcattaac cacgatacca tcgacggtga tttcaccttg ctgcactggt
    20161 tcgaggccgt tgacggtttt aatcagcgtt gatttgccgg aaccagacgg gccgcaaacc
    20221 accaccactt cgcctttttt cacttcggtt gagcagtcgg tcagcacctg aaagtgacca
    20281 taccattttg aaacattttt cagggtaatc attatgctgt ccttcttttc aagtagctga
    20341 ccaacaacga cgcgctaaga ctaataacga aataaacaaa tccggcaaac aggatcatct
    20401 caacctgcgt accatcacgc tcaccaatgg ttgaggcggt acggaagaaa tcggccagag
    20461 ataacacata caccagtgag gtatcctgga acagtacgat gccctgagtg agcagaagcg
    20521 gcaccatcgc gcggaacgcc tgcggcagaa taatcagttt catcgactgc cagtgagtca
    20581 ttcccaacgc cagcgcggcg ctcgactgac cacgagaaat actttgaata ccagcacgga
    20641 taatctctga atagtaggcc gcttcaaaca tcgaaaacgc caccatcgcc gaaattaaac
    20701 ggatatcatt ttttggtgat aatcccagca cgttttgcag aaaacctggc acgatcaggt
    20761 aaaaccacag caaaaccata actaaaggaa tcgagcggaa tacgttaacg taggctttgg
    20821 caaaccacgc tacgggcgca aagctggaca agcgcatcac cgccagcatg gtgccccaca
    20881 aaataccaat cacgactgcc gtgacggtga ttttcagcgt gatcaccagc ccgtcgagca
    20941 gatatggcag ggaagggaca atggaactcc agtcaaactc gtacattatt tgccccccat
    21001 gttgccaggc aggcgaactt tacgttcaac cagcgtcatt accagcatga taaccgcgtt
    21061 aatcaacaca tacgccagcg taatagcggt aaacgactcc caggcgtggg ctgagtaatc
    21121 aagcaattta cccgcctgcg ccgccatatc caccagacca atagtcgagg cgatggcgga
    21181 gtttttcacc aggttcatca tctcggaagt catcggcggg acgataacgc gatacgcgtt
    21241 aggcaacagc acgtatcgat aagcctgcgg tagcgtcagg cccatcgcca gcgcggcatt
    21301 tttttgccca cgcggcaacg actgaatggc ggcgcgtacc tgttcgcaaa cacgggcggc
    21361 ggtaaacagc cccaggcaga gcatggatga aaggaaaaac tgaatattgg gatccagctc
    21421 ggccttaaac cacatgccga ttttctccgg caacagctcc gggatcacca gataccaggt
    21481 aaagaattgc acaatcaacg gcacgttgcg gaacagttcg acatacaacg tacccagacc
    21541 ggagagaaaa cggtttggaa cggtacgtaa aatgccgaaa aatgaaccga cgaggaaagc
    21601 gataatccag gcgcagatcg acaaagcgat cgtcacctga aaaccactcc agatccaacc
    21661 aagataggtg gtgttgccga acggggcttg ttgtaaaaaa ataccccagt tccagtctat
    21721 agacataaat ctactccaga aaaaagaggg tagcagcgtt aactgctacc ctcgaagatt
    21781 gttacccagc gtattgcggt tttcaggccc gatggggaac gaccatcagg cgtatagtct
    21841 gtccgtgcta cgtaacaatc gagagggctg gaatttccgc ccctggttct tgtaattagt
    21901 tcagtgcttt gtcattcggt tctttgaaca gtgctttcat ttcgtctgac agttcgaaat
    21961 tcatgttcag gtttttcggc ggaattggat ttttgaacca tttatcaaac catttttccg
    22021 cttcaccgga agtctgcacc tgagcgatag tgtcatccat cagctttttg aactgcggat
    22081 catctttacg caacatgcaa ccgtaggctt cctgagactg cggcttgccg acgatatccc
    22141 agttgtctgg tttcttcgct ttcgcacgtt caccggccag cagagcgtca tccatcataa
    22201 aggcaacggc acgaccgctt tccagggtac ggaaagagtc accgtgatct ttggcgctaa
    22261 tgatgcgcat attcattttt tgctcttcat tcagtttgtt gagcaaaact tcagaagtag
    22321 tgccggaagt gacgactacg gctttgcctt tcaggtcggc aaaatctttg atatcgccgc
    22381 cctttttggt cagcaggcgc gtaccgacca cgaaaatagt atcagagaaa gccgcctgtt
    22441 tttggcgttc gacgttgttg gtggtagaac cacattcaaa atcgaaagtg ccgttttgca
    22501 gcagtggaat acggttttgt gaggtaatcg gaatcagttt tacctgcaag tccggtttgt
    22561 tgagtttctt tttcactgct tcaacaatgg cgttggagta atcctgcgag taacccacca
    22621 ctttttgctg attgtcgtaa taagagaaag gtactgaaga ttcacggtga ccgacgacaa
    22681 tcacgccgtt tttggcgatt ttgtccagcg tactgcccgc taccggggcg gcgtcatctg
    22741 cctgtgccag tcctgcggaa agcgccaggg cgaggattgc tgtggcaggt ttacgtaatt
    22801 gcatatccaa ctcctttatc ttctgcgtta agaacgcatg gatacccgtt gtgagtgttt
    22861 gtgttgttat cgtctgcaac tttattgtgc agtgttgtgc ttgttaggta acatttagtt
    22921 tggctaaatg taaagatatt gatgttttat tgtttgtttt tgcgaggtgc accgcaccat
    22981 tccgaagcaa aattcttaaa atgcactctt ttggtgctac cgctgatttg tggtggtgca
    23041 acccggttgc gtcgcggctg atttcattgc ttacacagta aataaagcaa cttacatgcc
    23101 aaaaaatact tatgggtgaa ttaattacga ttgtaatttc aggctgaaaa agtcatattt
    23161 cggacagagt tatcttttcg gcatgggacg tttgccggga tgataactgc acacgggaaa
    23221 aatgcttttt acctgtcttt tcaaggaaga aacaccttat gtctgcatct tcggttaagc
    23281 cgttaaatgt tcaattaccc gcaataaccc ttatcctttt tgcgctctgt gttgggatat
    23341 tttgttacct cgcacaatgg atgagttatg aagaagtcga tcaatccgca ctcatccatc
    23401 tcggtgctaa cgttgcttct ctcacgttgt cgggtgaatc ctggcgctta tttagcagtg
    23461 tctttctgca cagtagtttt tcccatttgc tgatgaatat gtttgcactc ctggtggtgg
    23521 gggcagtggc ggaacggata ctgggaaaat ggcgacttct gattatttgg ttattctccg
    23581 gcgtctttgg tgggctcatc agcgcctgtt atgcgttacg cgatagtgat cagatagtca
    23641 tcagcgttgg ggcgtccggg gcaattatgg gaatagctgg cgctgcgtta gcaacacagc
    23701 ttgcttcagg tgcgggcaca taccataaaa accagcggcg agtattttct ctgttgggta
    23761 tggtggcgct gacactgttg tatggtgccc ggcaagcagg aatagataat gcttgtcaca
    23821 ttggcggcct gattgcgggt ggcgcaatgg gttggcagag agcgcgttta tctgggcaaa
    23881 accggcttgt tacggaaggt gggattattg ttgcgggtag tcttcttctg accggggcta
    23941 tctggcttgc gcagcagcag attgatgagt cagttttaca agtcaggcaa agcctgcgtg
    24001 aagagtttta tccgcaggag attgaacaag agcgacgaca aaaaaaacaa cagttagcgg
    24061 aggaacgcaa cgccctcagg gaaacattat ccgctccggt aagtcgtgaa caggccagtg
    24121 gtgatttgct cgctgagatt gccgatatcc atgatatggc gatcagtcgg gatggtaata
    24181 cgttgtatgc cgcaattgaa gacaccaaca gcattgttgt tttcgacctc ggacaaaaga
    24241 aaatcctgca tacctttaca gcgcccatag cgaaagaaaa gtcagtcaaa cattgtggtg
    24301 gctgtaaaga tcagggcgtc agatcgctgg cattaagccc ggatgaaaag ttgatttatg
    24361 cgacttcatt tgaagcgaat gcgttatcgg tcattaacgt ggcgacaggg gagattattc
    24421 agtcgattac caccggtgca catcctgaca gtcttatcct ctcgcgtgat ggtacaaaag
    24481 cctgggtgat gaatcgcacc agtaatagtg tgtcagcgat tgatctggtg acttatcagc
    24541 atgtggcgga tatcccgctg gagaaatacg acgggacggg gacgagtaat aaacctggtg
    24601 cctgggttat ggctctttcc ccggatgaaa aaatattgtt gatacccggt atggtcagag
    24661 gtgacattgt acgcatcaat accatcacgc atcagaaaga aagctatccg gctagtgatg
    24721 cgcgaggaac gatatcggcg atgcgttttc gacctgaaaa cggggatgta atttttgccg
    24781 acagccaggg gatttcacgt ataagcgtag gggatcaaca agccagcatt atgacgcaat
    24841 ggtgtagcag gagcgtttat tccgttgagg gtattagccc ggacggtcag tatttagcgt
    24901 tggtgtcata tggcctgcaa ggttatgtca tcctgctcaa tattaatgcc gggcagatta
    24961 ttggcgttta tcctgccagc tacgttaatc accttcgttt ttcagcggat ggtagaaaaa
    25021 tatttgttat ggcgaagaac gggttgatcc aactggacag gacgcgctcg cttgatccgc
    25081 aggcaattat tcgtcatccc caatatggca atgtggcttg tatccctgaa ccgtaagaaa
    25141 aaggggcatg ataaccatgc cccaaagctc aatgagaaag tacgttttgc tcatgcagga
    25201 tacatcaggc acatcaacgt ttacgtgctc gcagactcat cagcacagcg gcaaaaccaa
    25261 acagtgccgt cagcacccac agcggccagt tgccggtacg tgcgtatggt gtgagtccgg
    25321 tggtcggcgt cacattagtg gttaacacct cgcgggtgaa ctgcgggatc atcgcctgaa
    25381 tctcaccctg cgggccaatc accgccgtaa tgccgttgtt ggtgctgcgc aacagtgggc
    25441 gcgccagctc cagcgcacgc attcgcgcca tctggaagtg ttgccatgga ccaatagatt
    25501 taccaaacca cgcatcgttg gagatagtca gcagatagtc ggtatccggg cggaagttat
    25561 cgcgcacttg ctcgccaaga atgatctcgt agcaaatggc cgcagtaagc tcaataccat
    25621 ttaccgacag cggcggctgg atatatggcc cacggctgaa cgacgacatc ggcagatcaa
    25681 agaacggtgc taacggacgc agaatcgact ccagcgggac aaactcgcca aacggcacca
    25741 gatggttttt gttatagcga tcggctgatt cgtagctgta cggtgcacct ttacccagtg
    25801 tgatgatggt gttgtaggta tcgtagcggt tctgcttatt gagacgcgcg tcgacaatcc
    25861 cggttacgag cgagctacct ttatcgcgca actcaccgtc cagtgctttg aggaacggtt
    25921 gctgattaat ttccagatcg gttatcgccg actccggcca gataatcaac gatgatttgc
    25981 ccatcagcgg agccgttgcg ttgtagtaaa tcttcagcgt attaagaagc tgatctccat
    26041 cccatttcag tgattgcgga atatcgccct gaaccatcga aacctgaatg gttttctccg
    26101 gttgtggggt aaaccattgg atgtaacgca gcgggaaggg tagggcaaac agcacgacgg
    26161 ccaccaccag cggacgccag ttgcgtttga ccaacgccag tgccagcagg ccactaacca
    26221 tcatcagcag gaagttaatg gcttccacgc ccattatcgg tgccagccct tttaacgggc
    26281 catcaatctg gctatagccg aactgtaacc acgggaagcc tgtcagtacc caaccgcgca
    26341 gaaactcggt cacttgccag agagaagggg cggcaatcgc cacgcgcagc caggtggttt
    26401 tcggccacag acgcgacagc acgccagcaa acagtccggt atacagcgac aaatacgccg
    26461 ccagcagcac caccaggaag atgttaaccg ggccaggcat cccgccaaag gtcgcgatgc
    26521 tgacatagac ccagttaata ccgctgccaa agaggccaaa tccccagcaa aagccaatag
    26581 cggcagactg gagtggacgg cggttaaagg tcaacgcctg aagccccatc aacgaaataa
    26641 tcgccgcagg ccagacgtcg taaggagaga aggccagcgt tccgcaggca ccgaataata
    26701 acgccagcag caggcgaatg cgctggcgtt caattaatga ggcaaaagcc atgtagttat
    26761 ctatccagtt tcggttttat tcatccagct tcggctgggg tgagtcatcc gggattttga
    26821 catgaacctg aataatacgc cgactgtcgg ccatcgccac tttgaactgg taaccgtcga
    26881 tgtcgatagt ttcgccacgc gccggaagat gcccaaatgc ctgcatcacc agaccaccga
    26941 tagtgtcaac ctcttcatcg ctaaagtggg tgccgaacgc ttcgttgaag tcttcaatgg
    27001 aagccagtgc gcgcacggtc caggtatgac gactcagctg acggaagtcg atatcatctt
    27061 cttcgtcata ctcgtcttca atctcaccaa caattagttc caggatgtct tcaatggtca
    27121 ccagaccgga aaccccaccg aattcgtcaa taacgatcgc catgtggtaa cgctgagagc
    27181 gaaactcttt cagcatccgg tctacgcgct tactttcagg aacgacaacc gcctgacgta
    27241 acactttgtc catgctgaag gcttcagcat cgctgcgcat aaacggcagc aagtctttcg
    27301 ccatcagaat cccttcaatg tgatctttgt cttcgctaat caccgggaaa cgtgagtggg
    27361 cggactcgat gatgacatca aggcattcgt ccagcgtctg gttgcgtttc agggtaatca
    27421 tctgggagcg ggggatcatg atgtcgcgaa cgcgttggtc tgcgatgtcc atcacccctt
    27481 cgagcatatc gcgcgtatct tcgtcgataa ggtcgttctg cccggaatca cggatcagcg
    27541 ccagcagttc gtcacggttt ttcggttcac cgtggaaaag ttggctgagt aacagggaga
    27601 aaaatccctt cttgttgctt atcgtgtcac tactgtgtga attgtcgtcg ctcatggcgt
    27661 cgtatgggtt ctcatgttag ttaatcaaaa cgccgtcgtt aatcaccaac ggcggggacg
    27721 tctgccagtc aaatgcctgg caaattattc tttctcggca atgtacggat cctcatagcc
    27781 cagagcaagc ataatctctg tttcgagggc ttccatttct tctgcttcgt catcttcgat
    27841 gtgatcgtaa cctaacaaat gcagactgcc gtgcaccacc atatgcgccc agtgcgcctc
    27901 cagtggtttg ccttgctcct gagcttcctt ctcaaccacc tgacggcaga taaccagatc
    27961 gcccagtagc gacatctcca tgccaggcgg cacttcaaac gggaaggaga gcacgttggt
    28021 cggcttatcc ttaccgcgat aggtcagatt cagactgtgg ctttcggcgg tatcgaccac
    28081 gcgaatagtc acttccgatt cttcctgaaa ctgcgggatc accgcattca gccatgtttg
    28141 aaactggctc tcttccggta acccggaatt atcttcacat gccagttgta aatcgaggat
    28201 cacctgactc atttttgttc ctgttcttcg cgcttgcgtt ctgccgccag cgccgctttt
    28261 cgtttttgtt cggcttcttc ccaggcttca taggcgttaa cgatacgcgc caccacaggg
    28321 tgacgaacca cgtcttcgct gtggaagaag ttaaagctga tctcttcgac atcggccagc
    28381 acttcgatgg cgtgacgtaa gcctgattta gtattacgcg gcaggtcgat ctgtgtgacg
    28441 tcgccggtga taaccgcttt tgagttaaaa ccgatacggg tcaggaacat cttcatctgt
    28501 tcgatggtgg tgttctggct ctcatcgaga atgataaacg cgtcgttcag cgtacgacca
    28561 cgcatatagg ccagcggcgc gacttcaata acgttgcgct caatcagttt ctcaactttc
    28621 tcaaagccca gcatttcaaa cagcgcgtcg tacagcgggc gcagatacgg gtctactttc
    28681 tggcttaaat cgccaggcag gaagcccagt ttctcaccgg cttcgaccgc cggacgagtc
    28741 agcagaatac ggcgaatctc ctgacgctcc agggcatcaa ctgccgcagc cactgccagg
    28801 taggttttac ccgtacccgc cgggccaacg ccgaaggtaa tgtcatggtc gagaatattg
    28861 gcgatgtact gcgcctggtt tggcgtgcgc ggcttaatta cgccgcgttt ggttttgata
    28921 ttgaccgctt tgccgtactc cggcacgctc tccgcacttt gctccagtac tcgcgcttct
    28981 ttaatcgcaa ggtggatctg ttccggttcg atatcctgaa tctgaccgcg catcggggct
    29041 gtatcgacat acaggctacg cagaatgtct gccgcagcgg tgacgcaaat cggacggccg
    29101 gtcagtttaa agtggttgtc gcggcgattg atctcgatgc cgagacggcg ttcgagctgc
    29161 ttgatgttgt catcaaacgg gccgcacagg ctcaacagac gcgcgttgtc tgctggctcc
    29221 agggtgattt cgcgagtgtc tatgttcaaa ccgttcctct tatctgtatg tcgccggaag
    29281 ctgaacattc accggcctat aaggaaatta ttcacgccac aggaaaaagg cgcaagcatt
    29341 gcgataaaga tggggataaa gagagaaaaa acaaggccca ccggaacggc gggcctgaga
    29401 attacggctg ataataaccc acgccaaggt cgttttcttt gcgggtacgg gcaatcaccg
    29461 attccggtgt ttctgccacg cgcagaccca tttcatcttc agtacgcacc actttaccgc
    29521 gcagagagtt cgggtagacg tcggtaattt ctacatcgac gaatttaccg atcatatccg
    29581 gtgtgccctc gaagttgacc acgcggttat tttcggtacg cccggaaagc tccatgatgc
    29641 tcttacgcga agtcccttct accagaatac gctgggtggt gccgagcatc cggcggctcc
    29701 acgccatcgc ttgctgatta atgcgctctt gcagaatata cagacgctgc ttcttctctt
    29761 cttccggaac atcatccacc atatcggcgg cgggcgtccc cggacgtgca gagaagataa
    29821 agctgtagct catgtcgaaa ttgacgtcgg caatcagctt catcgttttc tcgaagtctt
    29881 cggtggtttc gccagggaag ccaacaatga agtcagaact gatctgaata tccggacgcg
    29941 ccgcacgcag tttacggatg atcgctttgt actccagcgc cgtgtgagta cggcccatca
    30001 ggttcagaat gcgatcggaa ccgctttgta ccggcagatg caggaagctc accagctcag
    30061 gcgtgtcgcg gtacacttcg atgatatcgt cggtgaattc gatcggatgg ctggtggtaa
    30121 agcgaatacg atcgatcccg tcgatcgcag caaccagacg cagcagatcg gcaaacgatc
    30181 cggtggtgcc gtcgtagttt tcaccacgcc aggcgttcac gttctgaccg agcaggttga
    30241 cttcacgcac gccctgagca gcaagctggg caatctcaaa cagaatatcg tcagacggac
    30301 ggcttacctc ttcaccacgg gtgtaaggca ccacgcagta ggtacaatat ttattgcagc
    30361 cttccatgat ggagacaaac gcggtcggcc cttcggcgcg cggttccggt agacggtcaa
    30421 acttctcgat ttccgggaag ctgatatcta caaccgggct gcggtcgccg cgcacggagt
    30481 tgatcatctc cggcagacgg tgcagcgttt gcggcccaaa aataatatcg acatagtggg
    30541 cgcgctggcg aatgtgctcg ccttcttgcg atgccacgca gccaccgacg ccgataatca
    30601 ggtctggatt cttctctttt aacagtttcc agcgacccaa ctgatggaag actttttcct
    30661 gagccttctc gcggattgag caggtgttca gcagcagcac atccgcttct tccgccacgt
    30721 cggtcagttg atagccgtgg gtggcatcca gcagatcggc catcttcgat gaatcgtact
    30781 cgttcatctg acagccccag gttttaatat ggagtttttt ggtcatcgac ttgctcttgc
    30841 gaaatagtag ccaggaatgc agggcgcata gtgtaatgct ttgctgccgt tgtgaccagt
    30901 atgagcgtta tcagcccttg ggggtaaaaa tcctgtaaac ttaaagcata ttgctaacag
    30961 gatgattgac catgacaaat caaccaacgg aaattgccat tgtcggcgga ggaatggtcg
    31021 gcggcgcact ggcgctgggg ctggcacagc acggattttc ggtaacggtg atcgagcacg
    31081 cagaacctgc accgtttgtc gctgatagcc aaccggacgt gcggatctcg gcgatcagcg
    31141 ccgcatcggt atcattgctt aaagggttag gggtctggga tgcagtacag gctatgcgtt
    31201 gccatcctta ccgcagactg gaaacgtggg agtgggaaac ggcgcatgtg atgtttgacg
    31261 ccgctgaact taagctgccg ctgcttggct atatggtgga aaacaccgtc ctgcaacagg
    31321 cattgtggca ggcgctggaa gctcatccga aagtaacgtt acgtgtgcca ggctcgctga
    31381 ttgcattaca tcgagataat gatcttcagg agctggaact gaagggcggt gaagtgattc
    31441 gcgcgaagct ggtgattggt gctgacggcg caaattcgca ggtgcggcag atggcgggaa
    31501 ttggcgttca tgcctggcag tatgcgcagt cgtgtatgtt gattagcgta cagtgtgaga
    31561 acgatcccgg cgacagcacc tggcagcaat ttaccccgga cggaccgcgt gcgtttctgc
    31621 cgttgtttga taactgggca tcgctggtgt ggtatgactc tccggtacgt attcgccagt
    31681 tgcagaatat gaatatggcg caactccagg cggaaatcgc gaagcatttc ccgtcgcgtc
    31741 tgggttacgt tacaccgctt gccgctggtg cgtttccgct gacgcgccga catgcgttgc
    31801 agtatgtgca gccggggctt gcgctggtgg gcgatgccgc gcataccatc catccgctgg
    31861 cggggcaggg ggtgaatctt ggttatcgtg atgtcgatgc cctgattgat gttctggtga
    31921 atgcccgcag ctacggtgaa gcgtgggcca gttatcctgt cctcaagcgt taccagatgc
    31981 ggcgcatggc ggataacttc attatgcaaa gtggtatgga tctgttttat gccggattca
    32041 gtaataatct gccgccgctg cgttttgtgc gtaatctcgg gttaatggcg gcagagcgtg
    32101 ctggcgtgtt gaaacgtcag gcgctgaaat atgcgttagg gttgtagcct tacaacattg
    32161 ccggatgcgt gccaaccgta ggtcggataa gacgcggcag cgtcgcatcc gacattgaag
    32221 gataagacgt gtcaacatcg cattcgacat tgaatgaacg cagaaaagca aaaagctcgc
    32281 cgaagcgagc ttttttaatg tggctggggt acgaggattc gaacctcgga atgccggaat
    32341 cagaatccgg tgccttaccg cttggcgata ccccaactgg gcgcacttac gatgtaagcg
    32401 tcttgaataa attggctggg gtacgaggat tcgaacctcg gaatgccgga atcagaatcc
    32461 ggtgccttac cgcttggcga taccccaaca aattggtttt tgaatttgcc gaacgtattc
    32521 gacacattca gaatttggtg gctacgacgg gattcgaacc tgtgacccca tcattatgag
    32581 tgatgtgctc taaccaactg agctacgtag ccagattgtt tcttcgatgg ctggggtacc
    32641 tggattcgaa ccagggaatg ccggtatcaa aaaccggtgc cttaccgctt ggcgataccc
    32701 caataaccgg gcggtgaacc gcttactcga agaagatggc tggggtacct ggattcgaac
    32761 cagggaatgc cggtatcaaa aaccggtgcc ttaccgcttg gcgatacccc atccgtacaa
    32821 cgctttctgg tgaatggtgc gggaggcgag acttgaactc gcacaccttg cggcgccaga
    32881 acctaaatct ggtgcgtcta ccaatttcgc cactcccgca aaaaagatgg tggctacgac
    32941 gggattcgaa cctgtgaccc catcattatg agtgatgtgc tctaaccaac tgagctacgt
    33001 agccatcttt tttttcgcga taccttatcg gcgttgcggg gcgcattatg cgtatagagc
    33061 cttgcagcgt caacctcttt ttcaaggaaa attgctcgaa agtgactgtt tggttaggtt
    33121 gcgaacagcg tggcgctata ttcgtcaatt attgtttact ttgtgttttt tccccaccct
    33181 acagccattc ttttgtcgta caggatgaaa ttcggaattc aacaatagtg gtgggaaatt
    33241 aatctatgaa atactggcct acagtgatga gttgtcaaac agtgatgtgg caaacccgga
    33301 acatttcctt actgcatatc agaattaaca agcacctcaa taactgaaac agccccggat
    33361 ttcaccgggg ctgtttcgca tttcttactt atacgccgac tggtgaacgc caaccgcacg
    33421 accagacgga tcgtccattt tcttgaacgc ctcatcccac tcaatcgctt tagcggaaga
    33481 acacgcgacg gaaggaccgc ccggtacgca ctcagcggcg ctcggaagtg ggaacagttc
    33541 ttcaaagatc tcccggtaca gatacgcttc tttggacgtc ggcgtgttgt acgggaagcg
    33601 gaagcgggca gtttccagct gttgatcaga aacctgctgc gccgccactt ctttcagggt
    33661 gtcgatccaa ctgtaaccga cgccatcgga gaactgctct ttctgccgcc aggccacgct
    33721 tgcaggcagg tatgactcaa aacattcacg caggatgtgt ttttccattt tgccgttacc
    33781 gcacatttta tcctgtgggt taatacgcat cgccacatca aggaattttt tgtcgaggaa
    33841 cggaacgcgc gcttccacgc cccaggcgga catcgctttg ttggcgcgcg cgcagtcata
    33901 catatgcagg gccagcagtt tacgcaccgt ctcttcatgc agttctttag cgttgggcgc
    33961 tttatggaag taaagataac cgccaaacac ttcatcagaa ccttcaccgg acagcaccat
    34021 tttaatgccc atcgccttga tcttacgcga cattaaatac atcggtgttg aagcgcgaat
    34081 agtggtcaca tcataagttt cgatgtggta aatcacgtcg cggatggcat ccagaccttc
    34141 ctgtacagtg aagtgaattt cgtgatgcac cgtgcccaga tggtttgcca cttcctgggc
    34201 tgctttaaga tccggtgaac ccggcagacc tacagcaaag gagtgtaact gcggccacca
    34261 ggcttcagag cgttcctgat cttccacgcg acgggctgcg tatttcttgg tgatagcgga
    34321 aataattgag gaatccagac caccagaaag cagcacaccg taaggcacat cagacatcag
    34381 atggctttta acggaatctt ccagagcctg acgcagctcg tttttgtcgg tcacgttatc
    34441 tttcactgca tcaaagtcga accagtcgcg atgataataa gaacggattt cgccgtcctg
    34501 gctccacaaa tagctccccg ccgggaactc tttaatcgtg cggcaaactg gcaccagcga
    34561 tttcatttct gaggccacat acagctgacc gtgttcgtca tagcccatat acagtgggat
    34621 gatccccaga tggtcgcgac caatcaggta ggcatctttt tcgctgtcgt acagggcaaa
    34681 ggcaaacatg ccctgcaagt cgtcgagaaa ttccggccct ttttcctgat acagcgcgag
    34741 gatcacttca cagtcagacc ctgtctggaa ctgataacga tcgccatatt cagcgcgcaa
    34801 tgcctggtgg ttgtagattt caccgtttac cgccagtacg tgggtttttt gttggttgta
    34861 gagaggttgc gcccccgcgt taacgtcaac aattgacaga cgttcgtgag cgagaatggc
    34921 gttatcgctg gcataaatac cggaccagtc cgggccacga tgacgcatca ggcgtgacag
    34981 ctcgagtgct ttcttacgca gctcaactgc gtctgttttg atatcgaata cgccaaaaat
    35041 tgaacacata accttctccg ttaacttggt atttgttgct tgttgtgttt gcttgtttaa
    35101 aaaaatgccg caaagcagca ctgtgcgcaa gcgatttggc ggtgaaaaaa taaaaaacgt
    35161 aatggtgatt gctgatttgt gaaaaagaat agggtgtggt ggcgtattta ttgatggatc
    35221 gataattttt gacgggtttt attgaatggg atgattttaa tgatgggtaa aaataagaaa
    35281 ccacgtccgc agacgtggtt gctattcaga taacgtcgat ttcagcgacc gatgggtaaa
    35341 tccaacttgg gcggaacggc atactgtcga tatcgtcgag cgacgaaaca ccagaaagca
    35401 ccagaatcgt ctccagaccc gcctggaagc ccgccagaat atcggtacgc aagttatcgc
    35461 cgacaatcac cgtttcttcc gaatgcgcct gcattttgtt taatgccgcg cggatgatcc
    35521 acgggctggg cttaccaaca tagaacggtt tgcgcccgga gattttctca atccctgcac
    35581 acaacgcgcc acaagcggga taaaaaccgc gcccgtgggt gtccgggttg gtggcgataa
    35641 agcgtgcacc gttagcgacg aaataggccg ctttatgcat catgtcccag ttgtaggaac
    35701 gcgtttcgcc aacaatcaca aaatcagggt tcacatcggt aatagtgaaa ccggctttgt
    35761 acagttcatg aatcagtgcg ccttcgccca ccacatacgc tttcttgcct tcctggcgac
    35821 gcaggaaatc ggcagtcgcc atcgcagagg tataaaacac gctatccggc acatcgacac
    35881 cggcagtggc aaagcggttc gccagatctt gcccggtctg cgaaggatag ttggtcagca
    35941 acaccagcgg caggccttta tccataatcc cgtgcaaaaa ttccgctgca cccggtacgg
    36001 cgacgttatc gtgcatcagc acgccgtcga tatcgcaaat tacattttta atggtcatgg
    36061 actacccaga atattgacaa caataagcgc cactataaaa gcacattaat tttccagcaa
    36121 atgctggagc aaaataccgt tgagcatggc gcgttttacc agcgcaaaag cgccgattgc
    36181 cgagcggtga tccagctcag aacgtaccac cggcagatta gtgcgaaacg ccttcagcgc
    36241 ctgggtatta atgcagcttt caatagcagg gagcagcact ttatcggctt cggtgatttc
    36301 accggcaata acaatttttt gcggattaaa taagttgata gcaatggcga tggttttgcc
    36361 cagatgacga ccgacatact caatcacttc cgaggccagg ctatcgcctt tgttcgcggc
    36421 tttgcagata gttttgatgg tgcagtcgtc cagcggcacg cggctctggt agccctgctt
    36481 taacagattc aacacccgtt gttcaatagc ggcgttggca gcgatagttt ccaggcagcc
    36541 aaagttgccg cagtggcagc gttcacccag cggttcgacc tgaatatggc caatttcacc
    36601 gacgttgccg ttgcggccaa taaaaatgcg cccgttagag ataatcccgg ccccggttcc
    36661 gcgatggaca cgcaccagaa tggagtcttc gcaatcctga cttgcaccga agtagtgctc
    36721 cgccagcgcc agactacgga tatcgtgacc aacgaaacag gtcactttaa aacgttcttc
    36781 cagtgcttct accagccccc agttttctac ctgaatatgc ggcatgtaat gaattttgcc
    36841 gctgtccggg tcaacaagcc ctggcaggat caccgaaatc gcgatcagct cgcgcagttt
    36901 gcgctggtag ctatcaataa actgagcaat ggcattcaac agggcatgtt ccagcgtttg
    36961 ctgggtacgt tccggcagcg ggtaatgttc ttctgccagc actttgctgc tgagatcaaa
    37021 cagggtgatg gtggcgtcgt gacgaccaag ccgtacgccg attgcgtgga aattgcgggt
    37081 ttcggtgacg atggagatag cgcggcggcc cccggtggag gcctgctgat caacttcttt
    37141 gatcagcccg cgttcgataa gctgacgcgt aattttggtt acgctggcgg gggcaagctg
    37201 gctttgctcg gcaatctgaa tccgcgagat tggcccgtac tggtcaatca ggcgataaac
    37261 cgccgcgctg ttaagctgtt ttacgagatc aacattacct atctgagctt gtccgcctgg
    37321 tgtcatactt tctcttattg agttacgacc tcgttaccgt taacgatggt cttggtgatt
    37381 ttaaaatcag gtgtgaatgc ggtcaggttg gctactttac ctgcggcgag tgtgccgaga
    37441 cgtttctcaa cgccaatcgc acgcgccgga tagagcgtcg ccatacgcag cacttcatcc
    37501 agtgcgatac cgcaatgttc gaccagatta cgcacgcctt caatcatggt taaggatgaa
    37561 ccgcttaacg taccgttctc atccacacaa agtccgttac ggtagtatat tgttttaccc
    37621 gcaaaaatga actgttcaat gttggcacct gctggcgcgg tggcgtcggt aaccagacac
    37681 agtttgtcgc ctttaaggcg tttagcgtta cgaatgttgg cgtaatcaac atgcaggcca
    37741 tcagcgataa taccgcaata aatgtcagct tcgtcgagga tcgcgcccgc caggcccggt
    37801 tcacgaccgg taatatacgg catcgcgttg tacagatggg tggcaaaggt aatccccgcg
    37861 cggaaaccgg ctttcgcttc tttcaacgtc gcgttggagt gaccggcaga aaccacaatc
    37921 ccggcatttg ccagtttgct gatgacttcc gcaggaacca tttccggtgc cagggtcact
    37981 ttggtaatga cgtcggcgtt ttcacacagg aaatcgacca gcgcggcatc aggcttacgc
    38041 acaaaattcg gattatgggt gccttttttt accagattca gccacggacc ttccagatgc
    38101 agacctaacg cctgattcgg atgttttgcc aggtactcgc gcataacgcg cacgccctgt
    38161 ttcatcagct catcgctggt ggtgataagc gtcggcagat agttagtaca gcctgatttc
    38221 tcattggctt tctgcatgat ttccagcgtt tccacgctga ccgcttcagc ggtgtcgtta
    38281 aactgtacgc cgccgcagcc gtttaactgc acatcgataa aaccggggga gagaatggcc
    38341 ccgttcagtg aacgttgttc gatctctggc ggcagttccg ctaccggaca gacgctttta
    38401 atcaggccat cagcgataac aaccgcgtgg tcatcaagaa attcgtggcc ggtaaagatc
    38461 cggccctggg ttaatgcata cattctgacc cccgattttt aaaaatattg ccctgagcaa
    38521 ggagccaggg cagggataac aattacagac ctttgatatt ttctgcttct aattcattga
    38581 aatatcttaa agtcttaact ttcagctcca tggtggaagg ttcatcgcac accatgatcg
    38641 cttttggatg cagttgcaga cagctgatgg tccacatgtg gttcacgcaa ccttcaactg
    38701 cagcctgcag tgccagtgct ttctggctac ccagcaccag aatcatcact tcttcggcat
    38761 ccagcaatgt accaacaccg acagtcaggg catattttgg cacctgattg acatcgttat
    38821 caaagaaacg agagttcgcg acgcgagtgt catgagtcaa ggttttgata cgagtacgag
    38881 aagccagaga agacgccggt tcgttaaatg caatatgacc gtcgttacct acaccgccca
    38941 taaacagatg aatttttccg taagaacgga ttttttcttc atactggcgg cattcggcgt
    39001 cgatatccgg ggcgttgccg ttgagaaggt tgatgttttc tgctggaata tcaacgtgat
    39061 cgaagaaatt acggtgcata aagctgtagt agctttccgg atgctctttc ggcagaccga
    39121 catattcgtc catgttgaag gtgacaacgt gcttaaagct gacctggcct gctttatgca
    39181 tttcgactaa cgctttatag gtggtcatcg gcgtgccgcc agtcggcagg cccagaacaa
    39241 acggacgatc ggcagtcggt ttgaacgcat tgatacgatt gacgatatgg cgagcagccc
    39301 atttgccgac ctgttcagcg gtagtcaggg ggatcagtct cattattcac ctcaataagt
    39361 aaaatgtaag ccgttggcgg attaggcatc tttaagcgta acctggattt gcgcagacag
    39421 gcgtcaatcc gacctgattt tttgaatgat aaaataagtt ttctggttta gccagtaaaa
    39481 gggagtgatg ataacgatat ttggtgacaa aactcacaaa agacacgcgt ttaatttgcg
    39541 atacaaatta aattttcaca cactctgtag cagatgatct aacaatctga ttacagaaca
    39601 tcggcagtac aatttgcagc aaaataaaaa tacggcttta aacgagccaa atagggttct
    39661 cgtaggggga ataagatgaa tattttaggt tttttccagc gactcggtag ggcgttacag
    39721 ctccctatcg cggtgctgcc ggtggcggca ctgttgctgc gattcggtca gccagattta
    39781 cttaacgttg cgtttattgc ccaggcgggc ggtgcgattt ttgataacct cgcattaatc
    39841 ttcgccatcg gtgtggcatc cagctggtcg aaagacagcg caggtgcggc ggcgctggcg
    39901 ggtgcggtag gttactttgt gttaaccaaa gcgat
//
LOCUS       DAFATA010000031        39730 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFATA010000031 DAFATA010000000
VERSION     DAFATA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.031"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..365)
                     /locus_tag="LGJ20_002052"
     CDS             complement(<1..365)
                     /locus_tag="LGJ20_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386110.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RGINENFVGMDFTCEFRPE"
     gene            complement(654..917)
                     /gene="cedA"
                     /locus_tag="LGJ20_002053"
     CDS             complement(654..917)
                     /gene="cedA"
                     /locus_tag="LGJ20_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBK2386111.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRSKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            1100..3360
                     /gene="katE"
                     /locus_tag="LGJ20_002054"
                     /pseudo
     CDS             1100..3360
                     /gene="katE"
                     /locus_tag="LGJ20_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
     gene            complement(3407..4165)
                     /gene="chbG"
                     /locus_tag="LGJ20_002055"
     CDS             complement(3407..4165)
                     /gene="chbG"
                     /locus_tag="LGJ20_002055"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBK2386112.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVVRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRRERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(4178..5530)
                     /gene="celF"
                     /locus_tag="LGJ20_002056"
                     /gene_synonym="chbF"
     CDS             complement(4178..5530)
                     /gene="celF"
                     /locus_tag="LGJ20_002056"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK2386113.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMICKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5635..6477)
                     /gene="chbR"
                     /locus_tag="LGJ20_002057"
     CDS             complement(5635..6477)
                     /gene="chbR"
                     /locus_tag="LGJ20_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBK2386114.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6485..6835)
                     /gene="chbA"
                     /locus_tag="LGJ20_002058"
     CDS             complement(6485..6835)
                     /gene="chbA"
                     /locus_tag="LGJ20_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBK2386115.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKIKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6886..8244)
                     /gene="chbC"
                     /locus_tag="LGJ20_002059"
     CDS             complement(6886..8244)
                     /gene="chbC"
                     /locus_tag="LGJ20_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBK2386116.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8329..8649)
                     /gene="chbB"
                     /locus_tag="LGJ20_002060"
     CDS             complement(8329..8649)
                     /gene="chbB"
                     /locus_tag="LGJ20_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBK2386117.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8948..9286)
                     /gene="osmE"
                     /locus_tag="LGJ20_002061"
     CDS             complement(8948..9286)
                     /gene="osmE"
                     /locus_tag="LGJ20_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBK2386118.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9488..10315
                     /gene="nadE"
                     /locus_tag="LGJ20_002062"
     CDS             9488..10315
                     /gene="nadE"
                     /locus_tag="LGJ20_002062"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK2386119.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10545..11432
                     /gene="cho"
                     /locus_tag="LGJ20_002063"
     CDS             10545..11432
                     /gene="cho"
                     /locus_tag="LGJ20_002063"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBK2386120.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11392..11967)
                     /gene="ves"
                     /locus_tag="LGJ20_002064"
     CDS             complement(11392..11967)
                     /gene="ves"
                     /locus_tag="LGJ20_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBK2386121.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(12170..12655)
                     /gene="spy"
                     /locus_tag="LGJ20_002065"
     CDS             complement(12170..12655)
                     /gene="spy"
                     /locus_tag="LGJ20_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBK2386122.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12985..13953)
                     /gene="astE"
                     /locus_tag="LGJ20_002066"
     CDS             complement(12985..13953)
                     /gene="astE"
                     /locus_tag="LGJ20_002066"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBK2386123.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13946..15289)
                     /gene="astB"
                     /locus_tag="LGJ20_002067"
     CDS             complement(13946..15289)
                     /gene="astB"
                     /locus_tag="LGJ20_002067"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBK2386124.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15286..16764)
                     /gene="astD"
                     /locus_tag="LGJ20_002068"
     CDS             complement(15286..16764)
                     /gene="astD"
                     /locus_tag="LGJ20_002068"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBK2386125.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16761..17795)
                     /gene="astA"
                     /locus_tag="LGJ20_002069"
     CDS             complement(16761..17795)
                     /gene="astA"
                     /locus_tag="LGJ20_002069"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBK2386126.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17792..19012)
                     /gene="astC"
                     /locus_tag="LGJ20_002070"
     CDS             complement(17792..19012)
                     /gene="astC"
                     /locus_tag="LGJ20_002070"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBK2386127.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19458..20264
                     /gene="xthA"
                     /locus_tag="LGJ20_002071"
     CDS             19458..20264
                     /gene="xthA"
                     /locus_tag="LGJ20_002071"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK2386128.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20431..21141
                     /locus_tag="LGJ20_002072"
     CDS             20431..21141
                     /locus_tag="LGJ20_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK2386129.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            21146..21823
                     /gene="ydjY"
                     /locus_tag="LGJ20_002073"
     CDS             21146..21823
                     /gene="ydjY"
                     /locus_tag="LGJ20_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBK2386130.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21838..22545
                     /locus_tag="LGJ20_002074"
     CDS             21838..22545
                     /locus_tag="LGJ20_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK2386131.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22545..23093
                     /locus_tag="LGJ20_002075"
     CDS             22545..23093
                     /locus_tag="LGJ20_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK2386132.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            23103..24269
                     /locus_tag="LGJ20_002076"
     CDS             23103..24269
                     /locus_tag="LGJ20_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2386133.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            24242..25777
                     /locus_tag="LGJ20_002077"
     CDS             24242..25777
                     /locus_tag="LGJ20_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK2386134.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25777..26430
                     /locus_tag="LGJ20_002078"
     CDS             25777..26430
                     /locus_tag="LGJ20_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK2386135.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDSFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26497..27804
                     /gene="ynjE"
                     /locus_tag="LGJ20_002079"
     CDS             26497..27804
                     /gene="ynjE"
                     /locus_tag="LGJ20_002079"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBK2386136.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27813..28433)
                     /locus_tag="LGJ20_002080"
     CDS             complement(27813..28433)
                     /locus_tag="LGJ20_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK2386137.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            28520..28927
                     /gene="nudG"
                     /locus_tag="LGJ20_002081"
     CDS             28520..28927
                     /gene="nudG"
                     /locus_tag="LGJ20_002081"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBK2386138.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28893..29165)
                     /locus_tag="LGJ20_002082"
     CDS             complement(28893..29165)
                     /locus_tag="LGJ20_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBK2386139.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29401..30744
                     /gene="gdhA"
                     /locus_tag="LGJ20_002083"
     CDS             29401..30744
                     /gene="gdhA"
                     /locus_tag="LGJ20_002083"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK2386140.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30861..31901)
                     /locus_tag="LGJ20_002084"
                     /pseudo
     CDS             complement(30861..31901)
                     /locus_tag="LGJ20_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(32029..33990)
                     /gene="topB"
                     /locus_tag="LGJ20_002085"
     CDS             complement(32029..33990)
                     /gene="topB"
                     /locus_tag="LGJ20_002085"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK2386141.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(33995..35038)
                     /gene="selD"
                     /locus_tag="LGJ20_002086"
     CDS             complement(33995..35038)
                     /gene="selD"
                     /locus_tag="LGJ20_002086"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBK2386142.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(35155..35706)
                     /locus_tag="LGJ20_002087"
     CDS             complement(35155..35706)
                     /locus_tag="LGJ20_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBK2386143.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35867..37723
                     /gene="sppA"
                     /locus_tag="LGJ20_002088"
     CDS             35867..37723
                     /gene="sppA"
                     /locus_tag="LGJ20_002088"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK2386144.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRLIPAQQVFPGAQGLLVGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37890..38906
                     /gene="ansA"
                     /locus_tag="LGJ20_002089"
     CDS             37890..38906
                     /gene="ansA"
                     /locus_tag="LGJ20_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK2386145.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38917..39558
                     /gene="pncA"
                     /locus_tag="LGJ20_002090"
     CDS             38917..39558
                     /gene="pncA"
                     /locus_tag="LGJ20_002090"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBK2386146.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
BASE COUNT         9753 a        10491 c         9769 g         9717 t
ORIGIN      
        1 ttttcgggtc ggaattcaca ggtaaaatcc atcccaacaa aattctcatt aatacctctt
       61 atccgaaact cactgccata aagattatta attatcactc tatatttaac attgtcctgc
      121 agatacgtat caaaactaag tgctggcgct cctggctttg aggcatcata gttaatgtta
      181 gacatatagg caatcatacg attgaagttc gactctgcta tttcatcgca gtcacattga
      241 cccgttgata taaagaaagt gagtgccgcc atggtagact caagcctttc tttaaaagag
      301 tcgaaagcca ggcttttaat atttaaatca ccataattac tctgtattaa gtttgtagaa
      361 aacatctccc gccccatatt gttaacaaaa ttattatctc atttaaatct aagtcactta
      421 caatataagt ttaagagcga cgccacagga tgaactatca aaaatagctc atcatgatta
      481 gcaaaactta accattttaa aataaataaa caattaaaga aaaaagatca cttatttata
      541 gcaatagctc gtcaaaggca gctttttgtt acaggtggtt tgaatgaatg tagcaacgaa
      601 atgcagaatt tcaggtcatg taactcccgg caaaaccggg aggtatgtaa tccttactca
      661 gtcacttccc cttcctggcg gatctgattt gcccaacgtt gggcagattc aggcacagta
      721 aacgccggtg agcgcagaaa tgactctccc atcagtacaa acgcaacata tttgctacgc
      781 agcatccaga catcacgaaa cgaatccatc tttatcgcat gttctggcgg cgcgggttcc
      841 gtgcgtggga catagctaat aatctggcgg ttttgctggc ggagcggttt cttcattact
      901 ggcttcacta aacgcatatt aaaaattaga aaaactgtag tttagccgat ttagcccctg
      961 tacgtcccgc tttgcgtgta tttcataaca ccgtttccag aatagtctcc gaagcgggat
     1021 ctggctggtg atctatagtt agagagtttt ttgaccaaaa cagcggccct ttcagtaata
     1081 aattaaggag acgagttcaa tgtcgcaaca taacgaaaag aacccacatc agcaccagtc
     1141 accactacac gattccagcg aagcgaaacc gggatggact cactggcacc tgaggacgga
     1201 tctcatcgtc cagcggctga accaacaccg ccaggtgcac aacctaccgc ccctgggagc
     1261 ctgaaagccc ctgatacacg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc
     1321 agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca tcgccgatga tcaaaactca
     1381 ctgcgtgccg gtaaccgtgg tccaacgctg ctggaagatt ttattctgcg cgagaaaatc
     1441 acccactttg accatgagcg catcccggaa cgtattgttc atgcacgcgg atcagcagct
     1501 cacggttatt tccagccata taaaagctta agcgatatca ccaaagcgga tttcctctca
     1561 gatccgaaca aaatcacccc agtatttgtt aggttctcta ccgttcaggg tggcgctggc
     1621 tctgccgata ccgtgcgtga tatccgtggt tttgccacca agttctatac tgaagagggt
     1681 atttttgacc tcgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc
     1741 cccgattttg ttcatgcggt aaaaccagaa ccacactggg caattccaca agggcaaagc
     1801 gcccacgata ctttctggga ttatgtttct ctgcaacctg aaactctgca caacgtgatg
     1861 tgggcgatgt cggatcgcgg catcccccgc agttaccgca ccatggaagg cttcggtatt
     1921 cacaccttcc gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa
     1981 ccactggcag gtaaagcctc actcgtttgg gatgaagcac aaaaactcac cggacgtgac
     2041 ccggacttcc accgccgcga gttgtgggaa gccattgaag caggcgattt tccggaatac
     2101 gaactgggct tccagttgat tcctgaagaa gatgaattca agttcgactt cgatcttctc
     2161 gatccaacca aactgatccc ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg
     2221 ctcaatcgca atccggataa cttctttgcc gaaaacgaac aagcagcatt ccatccaggt
     2281 catattgttc ctggtctgga cttcaccaac gatccgctgt tgcagggacg tttgttctcc
     2341 tataccgata cacaaatcag tcgtcttggt gggccgaatt tccatgagat tccgattaac
     2401 cgtccgacct gcccttacca caacttccag cgtgatggca tgcatcgtat ggggatcgac
     2461 actaacccgg cgaattacga accgaactcg atcaacgata actggccgcg cgaaacaccg
     2521 ccgggaccga aacgcggcgg ttttgaatca tatcaggaac gcgtggaagg aaataaagtt
     2581 cgtgagcgca gtccatcgtt tggcgaatat tattcccatc cgcatctgtt ctggctaagt
     2641 cagacgccat tcgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc
     2701 gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg cgcatattga tctcactctg
     2761 gcccaggcgg tggcgaaaaa tctcggtatc gaactgactg acgaccagct gaatatcacc
     2821 ccacctccgg acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct
     2881 gacggtgatg tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca
     2941 gaccttctgg ccattctcaa ggccctgaag gccaaaggcg ttcatgccaa actgctctac
     3001 tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc cgctaccttt
     3061 gccggtgcac cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat
     3121 atcgctgaca acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg
     3181 attgcgctgg cgggagacgc gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt
     3241 gaagaaggga ttgtggaagc tgacagcgct gacggtagtt ttatggatga actgctaacg
     3301 ctgatggcag cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa
     3361 atatggataa ggtcacgtcc cctggggagt gaccttacca ccttacttac acatcaagat
     3421 aactacccaa ccgataacca cgctcagcaa ttgcatattt caacgacgct gatgtcagca
     3481 cttccagctc cgttaaccgt gggaaacagt aagcactctt gcgaatcaaa ttatcgacaa
     3541 acgccggatg gcacatgact tccagcgagc gttccctgcg atggctggaa tcatccagca
     3601 cttgcaggaa cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgcg
     3661 aactgcgcag attagccggt aaatcaccag cgttagatag cggctgacgg tcgatgcgca
     3721 acgcaatccc ctcttcagcc gcaaacctga caacaatcgg gaaaatctgc gggaacatat
     3781 gcacatgatg atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca
     3841 aatactgact ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca
     3901 actgccagat ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg
     3961 gcttgcccat agtaaggaca aagtgcatcc ctatggccag actcggttca tcacgactca
     4021 actgcaccgc atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc
     4081 cattgcgaca ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg
     4141 cattaacaat cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg
     4201 cgatgcagtc ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca
     4261 gcagctcagc atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat
     4321 cgttaaattc tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt
     4381 gaatcagccc catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat
     4441 cgcgccccag cttacagatc atttctaccg cccagtctgc cggaatatta tcaatatgcc
     4501 catgatgcgg gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca
     4561 cttcgcacgc tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa
     4621 ctttcaactc cggattttta tacagctcaa aaagttgttt ctcgactttc tgtactacct
     4681 gtgctcgtgc gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttctgct
     4741 tgaagtaata cagcagataa gaacatggca gcagatttaa cgaacgaatt aagccctcac
     4801 taaatggcag atcgaaaata ttttttacag aggacgcttt taactgccct gacgccacac
     4861 catcaagcaa ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca
     4921 tatggttgag gccgaacaga tcgatagata aatcatcgct gtctttcagc atcagaacat
     4981 cgcgaataaa catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc
     5041 cggtatgacg ataaacggct tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc
     5101 atgcattcgg acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac
     5161 gcagaccttt aaacagaccg cccgcgccgt tggtttcctg accaagataa ccatgactta
     5221 atggaatacg ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa
     5281 cgaaatcagc atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga
     5341 cgccagcgtt atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac
     5401 catcttcgac atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa
     5461 atccttccag taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt
     5521 tctggctcat aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc
     5581 ggacataaat aatccagcaa cagtacagat atgtgaattg tcaggtataa cgacttactg
     5641 attaaattca gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa
     5701 cgttttaata aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga
     5761 atagttggtc atttccagtt gttttttggc aaaattaata cggatctcat taataatctg
     5821 cattggtgtt ttgccataat atcgttgagt cgctcgcgtc aaatattcct gtgatttggc
     5881 tgacaacgtc accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt
     5941 ttctaccgta cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg
     6001 acgtaaacgg ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc
     6061 gcggaaattc aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg
     6121 gtatacctgc gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa
     6181 gcgtttactg atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg
     6241 gtgcgaacct aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt
     6301 aatctcctgg aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg
     6361 cagcccgctg atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa
     6421 caactgctgt tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga
     6481 ctccttatgc cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa
     6541 gcatggacgt cattaaatga tcctgagcgt ggaccagcac cagactcact ttaatcttac
     6601 cttcgcccgc atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca
     6661 tccgtgactg atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca
     6721 gtgccgcata cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt
     6781 cttccagctc ttcagcttct gtttgcgtat caggaatatt atcgagatcc atcatacatc
     6841 gttcctctct cttcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc
     6901 gttagcgata tcttcttcgc tttcttcttt atcaatcgca ttttgtgctt tgttagccac
     6961 cacaacaaag ggcagataaa ttaacgttgc gatgccaagg ttgaagagtg cgaccagcaa
     7021 tgcggcgacg ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg
     7081 tgcaatattg gtcaccggag gaataatgcc catgtagtac gctgcgaggg tgattgccgc
     7141 cagaatcggt tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag
     7201 accaaacaga atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac
     7261 ctgacgataa tcagcacggc gagatgcgat aaagatagcc aggatcaggc ctaaagtcgc
     7321 accactgcca ccgaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt
     7381 cttaccggct gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc
     7441 cagcgcccac ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat
     7501 accgaagaac cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa
     7561 cgatgccagt ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt
     7621 attcaacgcc caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc
     7681 cgagaaggaa cgcgatactg aagcaggtac gctgtcgggc agtttaatga cccaattgcg
     7741 gcggacaata aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat
     7801 attcgcccca cctaaccagt ttgcgccaac cgcataggcc tcaccgacac tatatggggt
     7861 gacggtcata aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc
     7921 ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc
     7981 gttatatacg ttgccgccaa tacctttcag accattaagt gtttcaatgg ttgaggcgtc
     8041 gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa aaacgttgtt
     8101 aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt
     8161 gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc
     8221 aagcgatgca ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatattg
     8281 cggtattgat ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc
     8341 ggctttttta atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata
     8401 aagcagcgag tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa
     8461 catataagca atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc
     8521 cagtgtttcc ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg
     8581 catttttgat accagtaaag aggtagacat gcccgcagaa caaaacagat aaatgtgttt
     8641 cttttccata aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt
     8701 aacctgcttc catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa
     8761 ggagtatacg gtatagacct gagcaatagt atttctttga ccttgtaaat tgtctctcct
     8821 tgacctgcgg ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga
     8881 actcaggcca aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac
     8941 gaagtgatta cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg
     9001 agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac
     9061 gttgacccag aatgtaggtc tggcaagtac cacgagcatg gatcatgctc acttcagacg
     9121 aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca
     9181 ccacaggctg tacaaactgg tctttggtac gatcataagc cgtacaaccc gccagcatgg
     9241 ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg
     9301 tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc ccgtgaacga
     9361 aacggctccg ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc
     9421 gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag
     9481 gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa
     9541 tgccgaagag gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc gaacttatcc
     9601 gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggtaa
     9661 gctgtgccag atggcgatta atgagctgcg ccaggaaacc ggcaacgaat cactgcaatt
     9721 tattgccgta cgcctgccct atggtgttca ggccgacgaa caagattgcc aggatgccat
     9781 tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggctag
     9841 cgagcaggca ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa
     9901 agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt
     9961 gggcaccgat catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg
    10021 tacggatatt aatccgctgt atcgtctcaa caaacgccag ggtaaacagt tgctgacggc
    10081 attaggttgc ccggaacacc tttataagaa agccccaacg gccgatctgg aagatgatcg
    10141 cccttctctg ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct
    10201 ggaagggaaa aacgtacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac
    10261 cgaacataaa cgccgtccgc caattaccgt tttcgatgat ttctggaaaa agtaataatt
    10321 tgcacataat ggtttgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc
    10381 cttattcgac ctacaaaact atgcaaattc aatatattgc aggagtcgaa taggcctgac
    10441 aggcgtagca cgtcagacgg tgtaaccttt gtcatcgacc cgcctctttt ttaatcgctt
    10501 cccgcctgtt acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa
    10561 cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacgtt
    10621 cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca
    10681 ccatgccgct ctatatcggc aaaagcatta acatccgcag ccgcgtcctt tctcatttac
    10741 gtaccccgga tgaagccgcc atgctacggc aatcccgacg catcagctgg atctgcaccg
    10801 caggcgaaat cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt
    10861 ttaataaacg gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg
    10921 tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt
    10981 ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa caaaaacttt
    11041 gttatggcct gttgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc
    11101 taaaacgttg cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct
    11161 tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt gcggtggcac
    11221 tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg
    11281 gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc
    11341 acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac
    11401 ttgatccggc gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg
    11461 ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag
    11521 caatttatcg cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc
    11581 aaaggtagta aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgtgt
    11641 catgatgttg aaatccatcg acatctgccc tgccgtcagt ttcgctttta ccacctggtc
    11701 cgccgcaaag gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc
    11761 aaggaacatc tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga
    11821 aaactcccca ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc
    11881 cggtgggaaa gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca gattcaccga
    11941 cattttacgc atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc
    12001 gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg
    12061 ctttttttac aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac
    12121 tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag
    12181 ttgcaggcat tttacctttt gccgctggac gttctgtcag acgcttctca aaattagcat
    12241 taaattgctt tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg gtttccatgt
    12301 gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct tcagcttttg
    12361 ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcggcgttct tccagcggcg
    12421 gacgtttcat ctggtcacgc tggcctttca tgatttcgcg gatctgctgt ttctgcgcgt
    12481 cggtcaggtt caggtctttg aacatcatgt cctgatgcgg accgaacttg cctttgtggt
    12541 gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt gtctgcggca tgggccaggt
    12601 tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta cgcatattct
    12661 atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt gttgacgaga
    12721 ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa
    12781 aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag tgatgagaga
    12841 ataatacaac acgatgattt tgcagagatt atgaagaact ataccggatg actggtgata
    12901 aataaagcaa ataaccagga ttaatctgta ttaatttata agaaagcaac ttaatacccg
    12961 cagaatgatt tctgcgggta agtattagct tattttttcg agcattaatc ccgcgcgtaa
    13021 tcccaacgct accaacggat tagggaataa cacatactct acatcatggg ttacggtaaa
    13081 acgttcctct ccgtcctgcg ccagcaatgt tcctttctca aacggcataa aattcagcgt
    13141 gtcacttgcc atatgcattt cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg
    13201 ataacggagc ggcggtgttc tcacgatacc gacactctca ccagatagca gcgcagcaat
    13261 tgcgctggca gttactgcaa actggcgaag atcgttttgc ccaaagggca acgctttgcc
    13321 aagttccagc gtacaggcca gcgcgccaaa atgtctggcg ctgaaatggg taaacgtacc
    13381 accaggttcc tgatggaaca ccagcgcctc cagccccgcc gcacccagcc acgtcagaaa
    13441 tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg aactgcggat gcaaggagcc
    13501 acgaattgcg gtatgtagat caaggtgcca gcgcacagat tctttgccca ggtcataaaa
    13561 atcttccagg cactgttcca gttcgcgcgc ccgacaggtt tctccgcttt cagcaaatag
    13621 ctgccaacga ccgccaaaca ttcgattcat atcgctatgg caataacgtt tcccttgctt
    13681 cagcgcagga ggattcccga ggatcaccag caaccgccaa cgtaacggga tctcgccgtg
    13741 agatatcgcg ccaagcaacg cgtccagcat ctccacaggt gccgtctcat taccgtgtat
    13801 tcccgctgaa atcaccagtg cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc
    13861 atcgcccagc cagcgccagc gaacgccgtt gatttctcgc tcggtgataa ccggtttttt
    13921 acccgttaag gtcagagcaa gaaaattatc cattgccccc tccctcgcgc tggaacggat
    13981 aaaccgaacc gagattcagt aattggctca atacatccag tgcttcccgc cctccgcgca
    14041 gcaattgcgg gtcggccaga tcggcagcag taaggcgatc gcggtagtaa cgatccaccc
    14101 agtcattgag cgcattaaac agcgtatcgt tcatcatcac cgccggattc accgcccggc
    14161 gttcttcttc tgtcaatacc acccgcaacc gcaggcacgc cgggccgccg ccattcgcca
    14221 tgctttcacg taaatcaaag acttttagtt cgctaatcgg gttgtcagcg gcaaggagtt
    14281 cattgagata accccatact ccggcgtgtt cccgacactc ctgaggcagc accaacatca
    14341 tggaaccatc atcgcggctc aacagttggc tgttaaacag ataggtagac accgcatccg
    14401 acacggaaac ctgagttgcc ggaacttcta tcgccataaa accgttcacc cgcgcacgca
    14461 ggtttgccag taactgtgac tggcgagcga acgcctgttg gtggcaaaac agcacctggc
    14521 ggttactcac ggcaatcacg tcattatgaa aaacgccctg gtcgataacg tccgggtttt
    14581 gctgggcgaa aatcacctgt tggggattca cctgattcag ccttgccacc gcctcgctgg
    14641 cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt atcattgcct tcttctcgcc
    14701 cgtagacaaa aagttgcata cccggctcac cgtaatgacc gccgagacga ttgtggtttg
    14761 ccgccccctc atcaccgagc aacgctacct gcggcaacgc cgaatgaacg ctaaatttct
    14821 cttcgtcgtt aaaaatcgct tttaaaagtg attcggtgac gtgcgcttcc agcgagcggt
    14881 gaaatttatt gttcaggttg gcaacggtga gatgcacttt gccatccaga gtatcggcag
    14941 atggcgcgat cgttgccgca ttggctaccc acattggcga agcggaactg acgctggaaa
    15001 gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg ctcatcgcta ccactgaatc
    15061 ccaactgacg cagcaccgga ataaacggac gctcgtgcgg cgggatcacg gcctggggga
    15121 atcccgcatc ggcaagggct ttcattttca gtaagccctg ttttgccgcc tgtcgcgggt
    15181 tagacacctg aaaacggtga cgggtagagg cttcattacc aaacgacagg ccagcgtaat
    15241 gatgcgtcag ccctaccagc ccgtcgaaat taacttccca ggcgttcatc gcaccacctc
    15301 atcggaaaaa tccagcccgg ggttaagcgt tgcgggcaaa gttaacgagt ccgactccag
    15361 gctcgccata ggccatgcgc agtaatctgc ggcataccag gcgctggggc gatggttacc
    15421 ggatgcacca atgccgccga atggcgcggt actggcagca ccagtaagcg gtttgttcca
    15481 gttaacaatc cccgcccgcg cctccagcaa cagttgatcg aatttttccc gctcggggga
    15541 aaccagaccg caagagagtc cgaagcgagt gttattcgcc attagaatcg cttcctcgaa
    15601 agaatcataa cgccagacgc gcaataacgg tccgaacacc tcttcatctg gtacgccagc
    15661 aacgcctgtc atttcaatga tccccggcgt cagcaacgat gtctctgatt gtaataagcg
    15721 cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc tgccaggcag taaccacctg
    15781 ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc tgcggttcgt catcccagtt
    15841 gcccggcgtt aatcgctggc tgacggcaac cagacgagca agaaacgcat cgccctgcgc
    15901 tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt tgcccggctg tgacaaacgc
    15961 cgactgaatg gtcagatgga cagccgcgtc gatatccgcc acctcatcga taattagcgg
    16021 attattaccg cccatctcaa gggcgagaat tttctccggc tgaccggaga gctggcgatg
    16081 cagctggtag ccggtattgg cgctaccggt aaacagcaaa ccgtcgagat cctccagcgc
    16141 actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg ttcagtacgc ccggcggcaa
    16201 gccagcctgc tgccataaac gcattaccgc ttcgccactc cacggtgtca gttcgctggg
    16261 tttaaagata atggtgttac ctgccagcaa tgccggaacg atatgtccgt tcggcaaatg
    16321 accagggaaa ttatacggcc caaacaccgc cagcacgccg tgcgggcgat gtcgcaggct
    16381 cgccgcaccg tccggcattt cactacgctg ctcgccggta cgaacgtgat acgccttaat
    16441 tgatatcgcg attttattga tcatcgccgt cacttcggtt gccgcttccc agcgcggctt
    16501 acccgtttct ctggcaataa tcgcggttaa ttcgccttta ttcctttcca gcaatccggc
    16561 aaagcgttcg acaacgacct gacgttcagc caatgagagc cgcgcccagc gcggaaacgc
    16621 cgcacgggct gcgcgacaag cctgcccgac ctgagcggca tcggcatcat tgccttgcca
    16681 taacacctcg cccgataccg gattacgctt cacacgcgat gcgccctggc ccgttatcca
    16741 gtcaccgtta atccataaag tcatgctgtt ttctcctctg cgcacaggcg caccagacga
    16801 acgcgatccc ctgcgtggca tttgagggca tccagttgtg cggcggttaa aatcaaacgc
    16861 tcggttgccg gatcggtacg cgccagcacc acgcggaaat ggtgataatt ttcattggcg
    16921 accaggcagg ccggaaaatc gccctgcgca ggctgccctt ctgccacttc caccagccga
    16981 cttttacgaa tggcgcgcac gcggtcgatg tcacactcaa gcgttggccc gccgtcgaag
    17041 atgtcgatat agttgcggta acgaaaacct tctttctcca gcaccgcgcg ggcaggcgcg
    17101 gtttgcggat gtacctgacc gatgacgtcc tgggcttcct gggataaaaa gtgggtatag
    17161 atcggatgtt tcggcatcag ttctgcaata aatgcctttt gcccggtgcc gcagagaaaa
    17221 tcggcgcggc taaaatccat cgaaaagaag cgtttaccga ggctttgcca gaacggtgaa
    17281 tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca ctttgtcatt aaacttgtcg
    17341 cgaaaagccg ccataaacat aaagcgcgat ttcgacagca aatagccgtt gccctctttg
    17401 cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc tgccggtgtg atcgttactg
    17461 agaaacagcg tcggcaatgc gttatagaca ttcagctctt ttgaggcgtg aaccaacgtg
    17521 ccgacgcgat agttgtacca gggatcgttc agcccaaccg ccacctcaat ggcacaaatc
    17581 cccgccacgg tgcctgtctc gctatcttcc agcacgaaca catagccctg ctcacttttg
    17641 ggcagttcgc cttgccaggt tttgattgcc ctttcgatac gcgccgaaag cgtggcttca
    17701 ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg caagctgcat cagcgccgag
    17761 acatctgatc gctcaacggg acggatgacc atcatgatga acctcggcta acaaagtgtt
    17821 cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc ttcttcgctg acattgagcg
    17881 caggcgcaaa acgcaccacg ttgccacccg caatcagtac catcacgcct gctttcgccg
    17941 cttcctgaga gatctgtttc gcttgcccgg cgtaatcggc attcagtaca cagccaatca
    18001 gcaaacctaa gccgcgaact tcactgaaca aaccatagcg gtgattaata gtattaagac
    18061 gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag catctctggt gtgttgatga
    18121 gctccagcac tttgcctgcc accgccgagg ccagcggatt accgccatag gtggtgccat
    18181 gagtgccaac ggtcatcacg cgggcgcact cttcggttgc caacaacgca ccgaccggga
    18241 aaccgccgcc cagcgctttg gcggtagtta acagatcagg cgtcacgccg tagtgcatat
    18301 aggcatacag ttccccggtg cgcccgacgc cggtttgtac ttcatcaaaa atcaacagcg
    18361 cattgtggcg gttacacaat tcacgcagac cttgtaaaaa cgcgttgctg gctggcacca
    18421 caccgccttc cccctggatg ggttcgacaa tcaccgcaca ggtagagtcg tcaatcagcg
    18481 cgctggcaga gttaatatcg ttatatgcag catgacgaat atccgccggc agtggcgcaa
    18541 aatcctgtga ataggctggc tgcccacccg cactgacagt aaacagcgtg cgaccatgaa
    18601 acgcattttt gaacgccacg atgccgctct tatggctgcc gtagcggtcg tgagcgaatt
    18661 tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc ggagttacaa aagaagacgc
    18721 gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg cagtaccggc tcgttggtgt
    18781 aaccgttgcc ggtatgccag aacttactcg cctgttcgtt cagcgcttca cgcagttccg
    18841 gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc gaagtcgata tactctttcc
    18901 cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg tataaagggt gccggagcgt
    18961 aaacaggtat catccattca tcaaagtttt cacgcgtaat tggctgagac atagcgacct
    19021 ctacagtaaa taattcgtta tttatatgtt aacaataagt aatgtttgcg ctgtaaatgt
    19081 agattgcagg gttcgtgcca gccagtgata aaagtgcata aacggcggag gctaactgga
    19141 aatcaaggag ttataaccaa accatatgca tttaaagtgc atataaagtg aatacgtttg
    19201 cgatgtgggt gaataaaaag aataaaaaac gcaatgttat gcagaagtaa aatataattc
    19261 tggaattgtg atcattgacg aaatttactg gaaattactg cgccattctg acgcagcgcg
    19321 caccaaaagc gggcattttt tgcgccatcg ttgacatcat taacaaccat cgatcaaatc
    19381 acttaacaac aggcggtaag caacgcgaaa ttctgctacc atccacgcac tctttatctg
    19441 aataaatggc agcgactatg aaatttgtct cttttaatat caacggcctg cgcgccagac
    19501 ctcaccagct tgaagccatc gtcgaaaagc accaaccgga tgtgattggc ctgcaggaga
    19561 caaaagttca tgacgatatg tttccgctcg aagaggtggc gaagctcggc tacaacgtgt
    19621 tttatcacgg gcagaaaggc cattatggcg tggcgctgct gaccaaagag acgccgattg
    19681 ccgtgcgtcg cggttttccc ggtgacgacg aagaagcgca gcggcgaatt attatggcgg
    19741 aaatcccctc gccgctgggc aatgtcaccg tgatcaacgg ttacttcccg cagggtgaaa
    19801 gccgcgacca tccgataaaa ttcccggcaa aggcgcagtt ttatcagaat ctgcaaaact
    19861 atctggaaac cgaactcaaa cgtgataatc cggtactgat tatgggcgat atgaatatca
    19921 gccctacaga tctggatatc ggcattggcg aagaaaaccg taagcgctgg ctgcgtaccg
    19981 gtaaatgctc tttcctgccg gaagagcgcg aatggatgga caggctgatg agctgggggt
    20041 tggtcgatac cttccgccat gcgaatccac aaacagcaga tcgtttctca tggtttgatt
    20101 accgctcaaa aggttttgac gataaccgtg gtctgcgcat cgacctgctg ctcgccagcc
    20161 aaccgctggc agaatgttgt gtagaaaccg gcatcgacta tgaaattcgc agcatggaaa
    20221 aaccgtccga tcacgccccc gtctgggcga ccttccgccg ctaatttagc agctctcctg
    20281 gctcaaactg ggtcaggaga attaaccttg agaaaaatca acaaactgtc agtaatgatt
    20341 tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt agttgtctga ccacttctct
    20401 attatcaagt ttgatatagg aaactccacg atgaacgctg agcgtaaatt tcttttggcc
    20461 tgtcttattt ttgcgctggt catttacgct atccacactt tcggtttatt cgatctgctc
    20521 accgatttac cccacttaca gacactcatc cgccagagcg gattgttcgg ttatagcctc
    20581 tatattctgt tattcattat tgccactctc tttctgttac caggaagcat actggtgatc
    20641 gctggaggaa tagtttttgg cccgctctta gggacactac tctcattaat tgccgccacg
    20701 ctcgcctcct cgtgctcatt cctgctggcg cgctggctgg gacgtgattt actgctgaaa
    20761 tacgttggtc atagccatac ctttcaggcc attgaaaaag gcattgcgcg taacggtatt
    20821 gattttctta ttctgacccg cttaatcccg ttgtttcctt acaatattca aaattacgct
    20881 tacggattaa ccacaatcgc cttctggcct tataccctta tttcggcact cacgaccctg
    20941 cccggtattg ttatttatac cgtgatggca agcgatctcg ccaatgaagg cattacgctg
    21001 cgctttattt tacaactctg tctggcgggc ctggcgctgt ttattctcgt ccagctcgca
    21061 aaactctacg cccgacacaa acatgtggat ctgtctgctt cgcgccgcag cccacttact
    21121 cacccaaaaa atgaaggata gaacgatgtc gcaacattat tcagtgtcat ggaaaaaagg
    21181 actggctgca ctctgcttac tggctgttgc cgggcttagc ggctgcgatc aaaaagagaa
    21241 tgcggcggca aaagtggaat acgacggact ttcgaacagc caaccgctgc gcgtcgatgc
    21301 caataaccat acggtaacca tgctggtgca aattaatggt cgtttcctca ccgacgacac
    21361 tcgtcacggt attgtgttta aagatggctc caacggacat aaatcgctgt ttatggctta
    21421 tgcgaccccg aaagcatttt atgaagccct gaaagaggca ggtggtacgc cgggcgaaaa
    21481 catgacgatg gataataaag aaacgactca tgtcacaggc agcaaactgg atatttcggt
    21541 caactggcaa ggggcggcaa aagcgtattc cttcgatgaa gtgattgttg acagtaatgg
    21601 caagaaactg gacatgcgct ttggcggtaa tttaacggca gcagaagaga agaaaacggg
    21661 ttgcctggtg tgtctggata gctgcccagt cggcatcgtc agcaatgcaa catacactta
    21721 tggtgcggtg gaaaaacgcg gcgaagtgaa attcaaaggc aatgcctcag ttctcccggc
    21781 ggataacacg ctggcaacgg ttacctttaa aatcaccgaa taaagccagg ataaaggatg
    21841 atgaagatac aatcgcgtaa aatctggtac taccgtataa ccctcatcat cctgttgttc
    21901 gctatgctgc tcgcatgggc gctgcttccc ggcgtccatg agtttatcaa tcgcagcgtt
    21961 gcggcgtttg ccgccgtgga ccaacagggt atagaacgct ttattcagtc ttacggcgca
    22021 ctggcggcag ttgtctcgtt cttgttgatg attttacagg ccattgctgc accgctacct
    22081 gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg cgttctgggg cggcttgctg
    22141 tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt ttatcgccag agtgatgggc
    22201 cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg acagtatgga cggctttttc
    22261 actcgctacg gcaaacacac catcctggtc tgtcggttat tgccttttgt ccctttcgat
    22321 ccaatcagct atgctgccgg tctgacttca atacgttttc gctcgttttt tatcgccacc
    22381 gggcttggtc aattaccggc gactattgtt tattcctggg cgggcagcat gttaacaggc
    22441 ggtactttct ggtttgtcac cggactgttt attctgtttg ccctgaccgt ggtgattttt
    22501 atggcgaaga aaatatggct tgagcgccag aagaggaatg cctgatgggt ttaccgccgc
    22561 ttagcaaaat tcctttcatt ttacgtccgc aggcgtggct gcatcgtcgc cattacggcg
    22621 aggtgctaag cccgattcgc tggtgggggc ggatcccgtt tatcttttat ttggtgtcaa
    22681 tgtttgttgg gtggctggag cgcaaacgct caccgctcga tccggtagtg cgatcgcttg
    22741 tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg cgtggatatc accagtatga
    22801 aagtcgccga gcgtaccggc agcaccgata aactgctggc agtggctgac tggcggcaaa
    22861 gcccgctctt cagcgatgaa gaacggctgg cgctggagta cgccgaagcc gcaagcgtaa
    22921 cgccgccaac ggtcgatgat gccctgcgca cccgactggc tgcgcatttt gacgctcagg
    22981 cgctcaccga actgacggca ttgatcggcc tgcaaaacct gtcggctcgt tttaattctg
    23041 ccatggacat tcccgctcag gggctgtgcc gtattccgga aaaacgttct taaggagaga
    23101 agatgcgcca ttgtgggtgg ttgctgggat tgttatcgct gttttctctg gcaacacatg
    23161 ccagtgactg gcaggaaatt aaaaatgagg ccaaagggca aaccgtctgg tttaacgcct
    23221 ggggcggcga taccgcaatt aaccgctatc tcgactgggt tagcggcgag atgaaaaccc
    23281 attacgctat aaacctgaag attgttcgtc tggcggatgc cgcagacgcg gtgaagcgca
    23341 ttcagaccga agctgctgcc ggacgtaaaa cgggcggctc ggtggatctg ctctgggtga
    23401 acggcgaaaa cttccgcacc ttaaaagagg ccaatttatt acaaacgggc tgggcggaga
    23461 ctctgcccaa ctggcgctat gtcgacacac agctgccggt gcgggaagat ttttcagtgc
    23521 ctacagaagg ggctgaatcg ccctggggcg gcgcacaact gacgtttatc gcccgccgcg
    23581 atgttacgcc acagccacca caaacgccgc aagccttact ggagtttgct aaagccaatc
    23641 ccggcacggt tacctatccg cgcccaccgg actttaccgg cacggcgttt cttgaacagt
    23701 tgctgattat gctgacgccc gatcccgccg cgttaaaagt tgccccggat aaagccactt
    23761 tcgcccgcgt cactgcgccc ttgtggcaat atcttgacgc gctacatccc tatttatggc
    23821 gcaaaggaaa ggatttcccg ccttcgcccg cgcggatgga tgctctgctg aaagccggca
    23881 cattgcgcct gtcgctgacc tttaaccctg ctcatgccca gcaaaaaatc gccagcggcg
    23941 atttgcctgc aagcagttac agttttggtt ttaaccaggg gatgattggc aacgtgcatt
    24001 tcgtcaccat tcctgctaac gcaaatgcca gtgctgcggc gaaggtggtt gccaacttcc
    24061 tgctctcacc caatgcgcaa ctgcgtaaag cggatcccgc tgtctggggc gatccttctg
    24121 ttctcgatcc gcaaaaactg cctgatgggc agcgcgaaac attgcaatca agaatgccgc
    24181 aagatctgcc gccggtactg gctgaaccgc acgcaggttg ggtaaatgcg ctggaacaag
    24241 aatggctaca ccgttacggt acgcattaat ctttttgctg tgggcgatgg tggcggtgat
    24301 ttatgcacca ctgatcccgg cagctctcac gctgatatcg cctgctttgt cgttgacaca
    24361 ctggcaggcg ttatttgccg atccacagtt accgcaggca ttactggcaa cgctggtgtc
    24421 gacaaccatc gcggcggtcg gggcgttgtt gattgccctg ctggtgattg tggcgctgtg
    24481 gccttggccg aaatggcagc gtatgtgtgc ccgtctgccg tggctgctcg ccattcccca
    24541 tgtggctttt gccaccagcg cccttctgct ctttgctgag ggagggctgc tttatgacta
    24601 tttcccgtat ttcactccgc taatggacaa attgggtatt gggctgggcc tcacccttgc
    24661 agtgaaagaa agcgcatttc tgctgtggat cttagcggca gtattgagcg aaaaacggct
    24721 gttgcagcag gtcattgtgc tggattcgct gggctacagc cgctggcaat gcctgaactg
    24781 gctgctcttg ccctccgtcg cacctgcact ggcaatggcg atgttggcga ttgttgcctg
    24841 gtcgctttcg gtcgtggatg tggcgattat tcttgggcca ggtaatccac cgacgctggc
    24901 agtaattagc tggcagtggt taactcaggg cgacgcggat caacaaacga aaggcgcact
    24961 cgccagtctg ctgctgatgc tgttactcgc cgcctacgtt ttgctgagct atctgctatg
    25021 gcgcagctgg cggcgcacta ttccccgcgt agatggcatt cgcaagcctg ccacaccttt
    25081 attaccaggc actacgctgg cgagtttttt acccttaacc ggtgtgctgt gtgtggttct
    25141 gctggcgatc ctcgcggatc agtcgacgat caatagtgaa gcactcatca acagcctgac
    25201 aatggggctg gtggcgacat tcatcgcttt gctcctgtta ctgctgtggc tggaatgggg
    25261 gccacagcgt cgccagttgt ggctctggtt gcccattcta ttgcccgctc tgccactggt
    25321 ggcgggccag tacacgctgg cgctatggct gaacctggat ggaagctgga cggcggtggt
    25381 ctgggggcat ctgctgtggg taatgccgtg gatgctgttt atcctgcaac ccgcctggca
    25441 gcgcattgat tcacggttaa ttttgattgc gcaaacactg agctggtcgc gggccaaaat
    25501 cttcttttac gtgaaatgtc cgcttatgct acgccctacg ctgattgcct tcgcggtggg
    25561 attttcagtc agtattgcgc agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc
    25621 gacgctcacc actgaggcgg tggcattaag cagcggcggc agcaatggta ttttcgccgc
    25681 ccaggcttta tggcaactgc tattaccgct tattattttt gccctgaccg cgttagtcgc
    25741 aaaatgggta ggttatgtca gacaaggact ccgctaatgc tctgcgtgaa aaatgtttcg
    25801 ctacgtttac cagaaagccg cttgctgaca aacgttaact ttacggtgga taaaggtgac
    25861 attgtcacgt taatggggcc gtctggctgt ggaaaatcca ctctgttttc atggatgatt
    25921 ggtgcactgg ctggacagtt ttcttgtaca ggtgagctat ggctcaatga gcaacggatt
    25981 gacatgctac ccaccgcaca gcgtcagatt ggcattcttt tccaggatgc actgttattt
    26041 gaccagttca gcgtcgggca aaatttactg ctggcgctac cgtcgacgct caaggggact
    26101 gccaggcgca atgccgtgaa agacgcgctt gatcgagcgg gactggctga aacttatcac
    26161 caggaccctg cgacattatc aggcggtcag cgagcgcggg tggccctgct gcgcgccctt
    26221 ctcgctcagc caaaagcgtt actcctggat gagccattca gccgtcttga tgtggctctg
    26281 cgcgatagtt ttcgccagtg ggtgttcagc gaagttcgcg aactggcgat cccggtcgtt
    26341 caggtaacgc acgatctcca ggatgttcct gctgatagtt ctgttctgga tatggcgcag
    26401 tggtcagaaa attacaacaa actgcgataa cgcaaagttt ttctcaatgc gtcagttcag
    26461 aatggcgcac tcaaaactac aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc
    26521 gcgctggcaa tggctttagg gctggcttgc gcttcttcgt gggccgctga actggcgaag
    26581 cctcttacac ttgaccagct tcaacaacaa aatggcaaag cgatagatac tcgccccagc
    26641 gcgttttata acggctggcc acaaacctta aatggccctt ctggtcatga acctgccgcc
    26701 ttaaacctct ctgccagctg gcttgacaaa atgagcaccg aacagctcaa cgcgtggatc
    26761 aagcaacata acctgaaagc cgatgccccg gtagcgctgt acggtaatgg caaagatgtc
    26821 gacgccgtca aaacgcgact gcaaaaagca ggttttacgc atatctccat tctgagtgac
    26881 gcgctaagcg aaccttcccg tctacaaaaa ctgccgcact ttgagcagct ggtttatccg
    26941 caatggcttc acgacctgca acaaggtaaa gaagttacgg cgaaacctgc cggtgactgg
    27001 aaagtcattg aagcggcctg gggcgctcca aaattttacc ttatcagcca tattcccggc
    27061 gctgactaca tcgataccaa cgaagtggaa agcgaaccac tgtggaacaa agtttctgat
    27121 gaacaactaa aagcgatgct ggcaaaacac ggcattcgcc atgacaccac ggtcattctg
    27181 tacgggcgtg acgtatacgc tgcagcgcgt gtggcacaaa ttatgctcta cgctggcgtg
    27241 aaagatgtgc gcctgctgga cggcggctgg caaacctggt ccgacgcggg tctgcccgtt
    27301 gagcgcggta cgccaccgaa agtgaaagcg gaaccggatt tcggcgtgaa gatcccggcg
    27361 caaccgcagt tgatgctcga tatggaacag gcccgtggac tgctgcatcg ccaggatgca
    27421 tcgctggtga gcattcgttc gtggccagaa tttatcggta cgaccagcgg ttacagctat
    27481 attaaaccaa aaggtgaaat agccggagca cgttggggac acgctggtag cgactcgacg
    27541 catatggaag atttccataa cccggatggc accatgcgta gcgccgatga tattaccgct
    27601 atgtggaaag catggaatat caaaccagat cagcaagttt cattctactg cggcaccggc
    27661 tggcgcgcgt ccgaaacctt tatgtacgca cgcgccatgg gttggaagaa tgtttccgtg
    27721 tatgacggcg gctggtacga atggagcagc gatccaaaaa atccggtagc aaccggtgaa
    27781 cgcggcccgg acagtagcaa ataacattgc aattactgac gctggtgcga cttcagcgtc
    27841 agatacccac tccacacccg cgtaaaggtc gtcatccagc acaacgcacc gaagatccac
    27901 gcaaaccacg caaaccatgc agggaataaa catcccagca caaacagtaa gatcgtttct
    27961 gtgccttcgg ttaacccgcc cagataataa aacgatttat gcgcataacc gggattatca
    28021 atctgatgtt tcgccgccag cgcagcaaaa gcgagaaaac tgctgcctgt gccgataaac
    28081 gcaaacagca gccagccgcc tgccagggcg ttttgctgcg gtgcggcgag gataaaccca
    28141 aaaggtacca gcgcgtaaaa aagaaaatcg agagaaatat caagaaagcc gcccgcgtcg
    28201 gtaagccctc tgcgccgcgc cagcgcaccg tccagaccat caagcagcct gttcaacaaa
    28261 atgacgacaa gcgccgccag ataccagccc agcgccagaa aaggcagtgc cagcacgccg
    28321 atggcaaatc ccactaatgt taaaccatct ggcgtaatgc ccggtttatc aagaacccgc
    28381 acgcactgat gcaacaacgg tttaatccgg ggatgaagat ggcggtctag cacaggcact
    28441 ccttaaatat aaagcctttc tgattgagca acagtgcgga tattatggca tttttcgctt
    28501 atctgcccgt gtgtaattta tgaaaatgat tgaagttgtt gccgccatca ttgaacgtga
    28561 tggcaaaatt ttactcgcgc aacgccccgc ccagagcgat caggcgggat tatgggagtt
    28621 tgccggtggt aaagtcgagc cggatgaaag ccagcggcag gcgctggtgc gtgagttacg
    28681 cgaagaactg ggcatcgaag caactgtggg tgagtatgtt accagccatc agcgagaagt
    28741 ttcgggccgg attatccatc ttcatgcctg gcacgtaccc gacttccacg ggacgttaca
    28801 ggcacatgaa catcaggcgc tggtctggtg ctcacctgaa gaggcgctgc aatatccgct
    28861 ggcccctgct gacattccat tattagaggc gtttatggct ttacgcgccg ccagaccagc
    28921 ggattagtgc taagggtttt gtcatcccgc tggcattgca gcagtattcc ctcggcttta
    28981 attaccgcgc cttcagaata atttttatcc tgataaacgc agcactgagt acagggctgc
    29041 gctgactgcc cgcctgaact gaacacttct ggcggtacgt ttacctccac gtccggacga
    29101 taatgcgggt tagccagtgc aattaatgga aatgctaata ctacggcgaa caatgctcga
    29161 ctcacaggga actccttaac gttattgtct ctgctactga taacggtagc cgggtggcaa
    29221 aactttagcg tctgagttat cgcatttggt tatgagatta ctctcgttat taatttgctt
    29281 tcctgggtca tttttttctt gcttaccgtc acattcttga tggtatagtc gaaaactgca
    29341 aaagcacatc acataaacaa cataagcaca atcgtattaa tatataaggg ttttatatct
    29401 atggatcaga catattctct ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat
    29461 caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa
    29521 caaaatccaa aatatcgcca gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg
    29581 atccagtttc gcgtggtatg ggttgatgat cgcaatcagg tacaggtcaa ccgtgcatgg
    29641 cgtgtgcagt tcagctcagc catcggcccg tacaaaggcg gtatgcgctt ccatccgtca
    29701 gttaacctct ccattctcaa attcctcggc ttcgaacaaa ccttcaaaaa tgccctgact
    29761 accctgccga tgggcggtgg taaaggcggt agcgattttg acccgaaagg taaaagcgaa
    29821 ggcgaagtga tgcgtttctg ccaggcgctg atgaccgagc tttatcgtca tctgggcgcg
    29881 gataccgacg ttccggcagg tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg
    29941 gggatgatga aaaagctctc caacaatacc gcctgcgtct tcaccggtaa gggcctttca
    30001 tttggcggta gtcttattcg cccggaagct accggctacg gtctggttta tttcacagaa
    30061 gcaatgttaa aacgccacgg tatgggtttt gaagggatgc gcgtttccgt ttctggctcc
    30121 ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact
    30181 gcgtcagact ccagcggcac tgtagttgat gaaagcggat tcacgaaaga gaaactggca
    30241 cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg cagattacgc caaagaattt
    30301 ggtctggtct atctcgaagg ccaacagccg tggtctgtac cggttgatat cgccctgcct
    30361 tgcgccaccc agaacgaact ggatgttgac gccgcgcatc agcttatcgc caatggcgtt
    30421 aaagccgtcg ccgaaggggc aaatatgccg accaccatcg aagcgactga actgttccag
    30481 caggcaggcg tactgtttgc accgggtaaa gcggctaatg ctggcggtgt agcaacgtca
    30541 ggccttgaaa tggcgcaaaa cgctgcgcgc ctgggctgga aagccgagaa agttgacgca
    30601 cgtttgcatc acatcatgct ggatatccac catgcctgtg ttgagcatgg tggtgaaggt
    30661 gagcaaacca actacgtgca gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg
    30721 atgctggcgc agggtgtgat ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc
    30781 tacaaatggg cacaattcat tgcagttacg ctctaatgca ggccgggcaa gcgcagcgcc
    30841 cccggcaaaa tttcaggcgt ttatgagtat ttaacggatg atgctcccca gggaacattt
    30901 cttatgggcc aacggcattt cttactgtag tgctcccaaa actgcttgtc gtaacgataa
    30961 cacgcttcaa gttcagcatc cgttaacttt ctgcgatagc agcagatatg ccagtaaaga
    31021 aatcccatta gactattttt ttgataatct tcttcgcttt cgaacaactc gtgcgccttt
    31081 cgagaagcaa gcattatata atgccaggcc agttcttctt caattgtccc gttttgaaaa
    31141 gctgtgcttg atatcgagat catctatgat aattccgccg cccatattag cttcgccgag
    31201 gatttaccgg agcgattatt agcgcaatca gagatatagt ctgagggaaa aacagcaaat
    31261 ttattcaaca aggcgataac ctgctctggg gcttcctcca tgtttgcttt aaaggtattg
    31321 gctccatggt cgccagaaag aaaatgctcc attaaggcac aataactttc gctatcttcg
    31381 ataccccatt gatcctctaa agactcgcgt cttttactta tgatatcgat cgagtcaaaa
    31441 ggaagcacat gatattggaa ggtatctttg ccaggttcag gctttcgcgg ccagaactcc
    31501 agcgtttcag accattgctt atgatagaat cgataaggtg cgatcaattg tagcgcctgt
    31561 aacttctcga tactgagcgg ctcaatacct ttagcctgat aataatgcag ttgttctttt
    31621 tttgctttaa aaccggcccg aacaataagc cccatcataa ttaatagata aagaaaagag
    31681 catcccgcgg taatcaggcc tctttcattc aaaccgttgg atgttatcgc tgcgaacaca
    31741 aacattacag cgacaacaca tgttaaataa aacccccact tacaaagcag catggcctta
    31801 ttttctttaa tcatccgttc aaaattacta ttaaatattt cccagccatt aaaagaatac
    31861 ttctcgctcc caggatggtt ttgtaataaa acttttttca tcacattccc tgttattaca
    31921 tactttagag gcgataattt tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc
    31981 gctggatatt tcagaatatg actcgaatag cacgaaagat tcactcgctt acgctatcgc
    32041 cccgcttccg acttcatctg ctggcggact ttttttcgca ctacgtttac gcggtgcagc
    32101 ctttttctta tcagcactgc caccactgcc cggagccaca atgccgcgaa actgccgcac
    32161 cggcgtacgt ttggcttgat caataagctg atatagcgtc cccaccagcg gctgcataaa
    32221 gtcctgatag cgacactgct tttcgctgat ttgcgtcagc accgattccc agtgcgcggt
    32281 catgtccggt cgcgtcgcca tttctggcag cgaatggaat aacgcttttc cggcgtcggt
    32341 agagtggata tagcgccctt ttttggtcag aaaaccacgc ttgaacagca gttcaataat
    32401 cccggcacgg gttgcttctg tccccagacc atcggtcgca cgaaggatct ttttcagatc
    32461 tttgtcctgc acaaagcgcg cgatcccggt catcgccgaa agcagtgttg catcggtaaa
    32521 atggcgcggc ggctgggttt gccgctctac cacttcacct ttttcacaca gcaactcatc
    32581 gcctttcgcc accacaggca atggcgtacc gtcgttttct tcatcgcgct ctttgctgcc
    32641 taacagcgtg cgccagcctg cttcagcaag aaaacgggct ttagcgacaa atttgccttt
    32701 ggcaatgtcc agttcgataa cacacttgcg gaacaccgca tccgggcaga actgcatcag
    32761 gtattgacgg gcaatcaggt tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga
    32821 actccgtgcg gtcgggatga tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg
    32881 gttgcgtata tctggatcta ccactggctg cggcaacaga tctggtgcat gaacgctgat
    32941 ggcattcatc accgcgtggc gtccggcaaa atgttcttct ggcaaatagc gacaatcgga
    33001 acgcggataa gtgattagct tgtgcgtttc gtacagcttc tggcagatat caagcacgtt
    33061 ctgcgcgctc agaccaaagc gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg
    33121 cagcggcgcg gattctgatt cccgtttatc gttatagctg gtgacaatag ccggttgacc
    33181 gctgatgcgg ttaaccacat gctccgccag tggacgatgt aacaagcgcc cttcttcatc
    33241 ctggtacggt tcacacgctt cgctcggttg ccagatagcg gtaaaccgct catcggcagg
    33301 tgtcacgata tgtgctttga cttcaaagaa gtctttcgcc acgaagtttt caatctcttc
    33361 atcgcggcgc accaccagcc caagcacggg cgtctgcacg cgtccgacgg aaagcacgcc
    33421 ctgataaccg gcattgcgac cgagaatggt gtaagcacgg gtcatattga tgccgtacag
    33481 ccagtcggca cgcgctcgcg ccagcgcaga aacgcacagc ggtacaaact cactgttgga
    33541 acgaagacgg tcgatcgccc gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg
    33601 ctgcacctgc tggcgctttt ccggtgccag ttgcagatag tccagcactt catccaccag
    33661 caattgccct tcacgatccg ggtccccggc gtgaacgatt tcgctggctt catgcaggaa
    33721 gcgtttgatg acgttaagtt gtttggtcac ggagggtcgt ggctgtaatt gccacttttc
    33781 cgggacaatc ggcaaatccg caagattcca gcgcgcatag cggctgtcgt aggcgtctgg
    33841 ctgcgcctgc tcaagcaggt gaccgataca ccaggtcacc acctgaccat taccgcactc
    33901 gataaagcca tcgcctttac ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag
    33961 actcggtttt tcggcaataa acaaccgcat tgaattaacg gatctcaacc atggcacgac
    34021 cgccgcgcgc tggcaccagt tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg
    34081 tagttttaac ctcattttct gcttccggca tgaccgccag cagcaaaccg ccagaagttt
    34141 gcggatcgca cagcagatcg cgcacttcac gcggcatttc acccatcaga tgaccgtagc
    34201 tggcaaagtt acgttcggtg ccgccaggca ctgcgcccaa cttaatgtac tcttcaaccc
    34261 ccggcaattt tgggatcgcg tcatagtcaa cgcgcgcctg cacacctgcg ccctgacaca
    34321 tttcgctcaa atggcccagc agaccaaaac cagtaacgtc ggtcatcgct tttacgcctt
    34381 cgatgttggc aaaggacgca cctgcgatgt tcatccggca catcacttcc gtcgccagtc
    34441 cctgatgttc tggtttcaac agtgattttt tctcagccgt ggtaagaacg ccgatcccca
    34501 gcggtttcgt caggaacagt ttgcatccgg cttgtgcggt actgtttttc ttcacccgct
    34561 cggtcggtac gatccccgtt accgccagac caaaaatcgg ctccggcgca tcgatggagt
    34621 gaccgccagc cagcgcaata cccgcctgac ggcatgcata gcgtccacct tcggttactt
    34681 cgcgggcaat ttctggggaa agtttgttaa tcggccagcc gaggatcgca atcgccataa
    34741 tcggtttgcc ccccatcgcg aagatatcgc tgatggcgtt agtcgccgca atgcggccaa
    34801 aatcgaaagg attatcaacg atcggcataa agaagtcggt ggtactgata acgctggtgc
    34861 cattgcccag atcgtacacc gccgcatcgt cgcgggtctc attacccaca agcaaattcg
    34921 gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc caacactttt ggggaaattt
    34981 tacagccgca accagctccg tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg
    35041 acatctcctg tcaatgcaat ccgggtatgg taaccctcat tccgtgaagt gataagtgag
    35101 agtgtctgaa ttcctgcgcc tttgctcaca atccagacag tttcgcgaca attatcagaa
    35161 ataagttaca aacggagtcg ggtccgggac gttaatcgac gtagatgctt tcaactgcgg
    35221 cgtgccgagg tagagaaaac cgacaatttt atcctgctca cggcaaccga atgcttcacg
    35281 cactaccgga ctttcagtta atgcgccact gcgccagatg ccgccaaatc cttgggcaac
    35341 ggctgccatt tgcatcgcca tgaccgcgca tccggcagac atttcctgtt cccatcgcgg
    35401 gactttatga ttttcttcgc atttcgccac caccgtgatg atgagcggtg cgcggaacgg
    35461 cgcattacgg gctttgtcga tagctttgtc atcactaccg gcagcaatcg ccccctgttc
    35521 cagtacggcg ctgaaacgct cgcgcccttc cccttcaatc acaaaaaaat gccacggttg
    35581 catggactta tggtccggcg cacgcatacc cgcacgcagg atgttttgca gttgttcacc
    35641 cgttggcgcg ggttcagcca ggcgggaggc gctacggcga ttgatcaata gttcgagtgc
    35701 atccatttga ttaactcctg tcgtgatatt tattcacaaa attaacacga gagtggattt
    35761 tgttacagca cagtccgcaa ttcctgctga caagtgccgg ttgggtcatt acgataacca
    35821 catctattgc gcctgtgaca ggtgtgacct taagttggga gaatacatgc gaaccctttg
    35881 gcgatttatt gccggatttt ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat
    35941 ggtacttaac ctgttcttta ttttcctcgt actggttggt gtggggatct ggatgcaggt
    36001 cagtggtggt gattcgaaag aaacggccag tcgtggcgca ctgctgctgg acatttctgg
    36061 tgtgatcgtc gataaacccg acagttctca gcggtttagt aaattaagcc gccagctgct
    36121 tggtgccagt tccgatcgtc tgcaggaaaa ctcactgttt gatatcgtca acaccattcg
    36181 ccaggcgaag gacgaccgca atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg
    36241 cggcgaccag ccgtctatgc agtacatcgg caaagccctg aaagagtttc gtgacagcgg
    36301 gaaaccggtt tatgccgttg gcgagaacta cagccagggg caatattatc tcgccagttt
    36361 cgccaataaa atttggctgt caccgcaagg cgtggttgat ctgcacggtt ttgctaccaa
    36421 cggtctgtac tacaaatcgt tgctggataa gctgaaagtt tccacccatg tgttccgcgt
    36481 gggtacgtat aaatctgccg ttgaaccgtt tattcgtgat gatatgtcac cggcagcgcg
    36541 cgaagctgac agccgctgga ttggtgagct gtggcaaaac tatctgaata ctgttgccgc
    36601 taaccggctg atccctgctc agcaggtatt ccctggcgcg caagggttgc ttgtgggttt
    36661 aaccaaaacc ggtggcgata ccgcgaaata tgcactggaa aacaagctgg tcgatgcact
    36721 ggcatcgagt gccgaaatcg aaaaagctct gaccaaagag ttcggctgga gtaagactga
    36781 taaaaattat cgcgccatca gttattacga ttacgcattg aaaacgccgg cagataccgg
    36841 tgacagcatc ggtgtcgtct tcgctaatgg cgcaattatg gatggcgagg aaactcaggg
    36901 gaatgttggc ggtgatacca ctgcggcaca aatccgcgac gctcgccttg acccgaaagt
    36961 gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat
    37021 tcgcgctgaa ctggcagcag cccgggcagc gggtaagcct gtggttgtat cgatgggcgg
    37081 catggcggca tctggtggtt actggatttc cacgccagct aattacattg tggctaaccc
    37141 cagcaccctg accggttcta tcggtatctt cggcgtgatc accaccgtag aaaatagtct
    37201 ggattcgatt ggtgttcata ctgatggtgt ctcaacttca ccgctggcgg atgtttctat
    37261 caccagggca ctgccgccgg aagcgcagca gatgatgcaa ttaagcattg agaatggcta
    37321 taaacgcttt atcacgctgg ttgctgatgc gcgtcattcg acgccggagc aaattgataa
    37381 aatcgcccag ggccacgtct ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag
    37441 tctcggggat ttcgatgatg cggttgccaa agcagcagag ctggcaaaag tgaaacagtg
    37501 gcatctggaa tactacgttg atgaaccgac cttcttcgac aaagtgatgg acaacatgtc
    37561 tggttctgtc cgggcaatgt tgccagatgc gttccaggcc atgttacctg caccgcttgc
    37621 ctcggtagcc tctaccgtta aaagtgaaag cgacaagctg gccgcgttta acgacccaca
    37681 aaaccgttat gcgttttgcc tgacctgcgc caacgtgcgt taagtcttgt actgagtggc
    37741 cgacagatcg tcggccacat tattttttac ctcgacgaat cctcttcccg ctgtctcgac
    37801 ccatatttcc ttatccgcat agtatcaggt gcgctcctcc tgcctcacgt atatactttt
    37861 gctctttcga tatcattcat atcaatatca tgcaaaagaa atcaatttac gtcgcctaca
    37921 cgggcgggac catcgggatg cagcgttccg agcagggtta tattccggtg tcaggtcatc
    37981 tacaacgcca actggcgctg atgccggaat tccatcgccc ggagatgcca gatttcacca
    38041 ttcatgaata tacgccgttg atggattctt ctgatatgac gccggaagac tggcagcata
    38101 ttgctgaaga tattaaagcg cactatgacg actatgatgg ttttgtcatt ctacacggta
    38161 ccgacacgat ggcgtatacc gcctctgcgc tgtcgttcat gctcgagaat ctcggtaaac
    38221 cggtcattgt gacagggtca caaatcccgc tggctgagtt acgctctgac ggacaaatta
    38281 atctgctgaa tgcgttgtac gttgcggcga attatccgat caacgaagta acgctctttt
    38341 tcaataaccg attgtatcgc ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg
    38401 cgtttgcctc tccaaacctt cctccgttac tggaagctgg tatccatatt cgtcgtttga
    38461 atacgccacc cgccccgcac ggtgaagggg aattaatcgt tcatccaatc acgccacaac
    38521 caattggcgt agtgacgatt tatccgggga tttctgctga tgtcgtgcgc aattttctgc
    38581 gccaaccggt gaaagcattg attctgcgct cctatggcgt gggtaatgcg ccacaaaaca
    38641 aagccttcct gcaggaatta caagaagcca gcgatcgcgg tattgtggtg gttaacctga
    38701 cacaatgtat gtccggtaaa gtgaacatgg gtggttatgc caccggtaac gccctcgccc
    38761 atgccggcgt aattggcggt gcagatatga ctgtagaagc tacgctaacc aaactgcatt
    38821 acctgctgag ccaggaactg gatactgaaa ccattcgcaa ggccatgagc caaaacctgc
    38881 gcggcgaact gacgccggat gattaaggag actgcaatgc cccctcgcgc cctgttactg
    38941 gtcgatttac aaaatgattt ctgtgctggt ggcgcgctcg ccgtgccgga aggtgacagt
    39001 acggtggatg tcgctaaccg ccttattgac tggtgccagt cgcgcggtga agcggttatc
    39061 gccagtcagg actggcaccc ggcgaatcac ggcagttttg ccagtcagca ctgtgtagag
    39121 ccttatatgc caggtcaact cgacggtttg ccacaaacct tctggccaga tcactgtgtg
    39181 cagaacagtg aaggcgcaca attacatccg ttactgaacc aaaaagcgat cgcagcggtg
    39241 ttccataaag gcgaaaatcc tttagttgac agttacagtg ccttttttga taacggccgt
    39301 cggcagaaaa cctctctcga tgactggtta cgcgatcatg aaatcgatga attgatcgtt
    39361 atgggcctgg ctactgatta ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt
    39421 tataaagtaa acgtgattac cgatggttgt cgtggcgtga atatccagcc ccaggacagt
    39481 gcgcacgcgt ttatggagat gtcagcagct ggggcaacgc tatatacgct ggcagactgg
    39541 gaagagacgc aggggtaata ttagttgaat tcatctctgg cctacatttc ctcaatgaca
    39601 ttgtaggcca gataatactt gtcagtgttg catttaacct acttgcagct aaaacacgac
    39661 gtcctaacaa gtatatttgc cctcccttaa taacaggctt aaatactaga taactcattc
    39721 acttaaacat
//
LOCUS       DAFATA010000032        37446 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFATA010000032 DAFATA010000000
VERSION     DAFATA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.032"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..657
                     /locus_tag="LGJ20_002091"
     CDS             <1..657
                     /locus_tag="LGJ20_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK2386147.1"
                     /translation="GVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQ
                     LKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALVKGEATAHLIEDIPTGI
                     YTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMKVGTLKILFHRETKEIL
                     GIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
                     "
     gene            complement(640..1557)
                     /gene="oxyR"
                     /locus_tag="LGJ20_002092"
     CDS             complement(640..1557)
                     /gene="oxyR"
                     /locus_tag="LGJ20_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBK2386148.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1809..3092)
                     /locus_tag="LGJ20_002093"
     CDS             complement(1809..3092)
                     /locus_tag="LGJ20_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2386149.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            complement(3159..4373)
                     /locus_tag="LGJ20_002094"
     CDS             complement(3159..4373)
                     /locus_tag="LGJ20_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBK2386150.1"
                     /translation="MKIIAADVFVTCPGRNFVTLQITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYTKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            complement(4871..6244)
                     /gene="argH"
                     /locus_tag="LGJ20_002095"
     CDS             complement(4871..6244)
                     /gene="argH"
                     /locus_tag="LGJ20_002095"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBK2386151.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(6305..7081)
                     /gene="argB"
                     /locus_tag="LGJ20_002096"
     CDS             complement(6305..7081)
                     /gene="argB"
                     /locus_tag="LGJ20_002096"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBK2386152.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(7089..8093)
                     /gene="argC"
                     /locus_tag="LGJ20_002097"
     CDS             complement(7089..8093)
                     /gene="argC"
                     /locus_tag="LGJ20_002097"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBK2386153.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            8247..9398
                     /gene="argE"
                     /locus_tag="LGJ20_002098"
     CDS             8247..9398
                     /gene="argE"
                     /locus_tag="LGJ20_002098"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBK2386154.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            9750..12401
                     /gene="ppc"
                     /locus_tag="LGJ20_002099"
     CDS             9750..12401
                     /gene="ppc"
                     /locus_tag="LGJ20_002099"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBK2386155.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            12584..14317
                     /locus_tag="LGJ20_002100"
     CDS             12584..14317
                     /locus_tag="LGJ20_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBK2386156.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            14466..15317
                     /locus_tag="LGJ20_002101"
     CDS             14466..15317
                     /locus_tag="LGJ20_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2386157.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(15304..15645)
                     /locus_tag="LGJ20_002102"
     CDS             complement(15304..15645)
                     /locus_tag="LGJ20_002102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBK2386158.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(15647..16525)
                     /locus_tag="LGJ20_002103"
     CDS             complement(15647..16525)
                     /locus_tag="LGJ20_002103"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBK2386159.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(16491..18788)
                     /locus_tag="LGJ20_002104"
     CDS             complement(16491..18788)
                     /locus_tag="LGJ20_002104"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK2386160.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAELTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(18839..19159)
                     /locus_tag="LGJ20_002105"
     CDS             complement(18839..19159)
                     /locus_tag="LGJ20_002105"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBK2386161.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(19174..20253)
                     /locus_tag="LGJ20_002106"
     CDS             complement(19174..20253)
                     /locus_tag="LGJ20_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBK2386162.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            20562..23063
                     /gene="ptsP"
                     /locus_tag="LGJ20_002107"
     CDS             20562..23063
                     /gene="ptsP"
                     /locus_tag="LGJ20_002107"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK2386163.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            23075..23737
                     /gene="fsa"
                     /locus_tag="LGJ20_002108"
     CDS             23075..23737
                     /gene="fsa"
                     /locus_tag="LGJ20_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK2386164.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            23748..24851
                     /gene="gldA"
                     /locus_tag="LGJ20_002109"
     CDS             23748..24851
                     /gene="gldA"
                     /locus_tag="LGJ20_002109"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBK2386165.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            25126..25743
                     /locus_tag="LGJ20_002110"
     CDS             25126..25743
                     /locus_tag="LGJ20_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBK2386166.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(25770..26675)
                     /gene="yijE"
                     /locus_tag="LGJ20_002111"
     CDS             complement(25770..26675)
                     /gene="yijE"
                     /locus_tag="LGJ20_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBK2386167.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(26769..28949)
                     /gene="katG"
                     /locus_tag="LGJ20_002112"
     CDS             complement(26769..28949)
                     /gene="katG"
                     /locus_tag="LGJ20_002112"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBK2386168.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(29278..30168)
                     /gene="metF"
                     /locus_tag="LGJ20_002113"
     CDS             complement(29278..30168)
                     /gene="metF"
                     /locus_tag="LGJ20_002113"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBK2386169.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(30517..32949)
                     /gene="metL"
                     /locus_tag="LGJ20_002114"
     CDS             complement(30517..32949)
                     /gene="metL"
                     /locus_tag="LGJ20_002114"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBK2386170.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLVGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(32952..34112)
                     /gene="metB"
                     /locus_tag="LGJ20_002115"
     CDS             complement(32952..34112)
                     /gene="metB"
                     /locus_tag="LGJ20_002115"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK2386171.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            34389..34706
                     /gene="metJ"
                     /locus_tag="LGJ20_002116"
     CDS             34389..34706
                     /gene="metJ"
                     /locus_tag="LGJ20_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBK2386172.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            34890..35498
                     /locus_tag="LGJ20_002117"
     CDS             34890..35498
                     /locus_tag="LGJ20_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBK2386173.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            35643..36318
                     /locus_tag="LGJ20_002118"
                     /pseudo
     CDS             35643..36318
                     /locus_tag="LGJ20_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(36405..36728)
                     /locus_tag="LGJ20_002119"
     CDS             complement(36405..36728)
                     /locus_tag="LGJ20_002119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386174.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFYESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRK
                     KSRMR"
     gene            complement(36730..>37446)
                     /locus_tag="LGJ20_002120"
     CDS             complement(36730..>37446)
                     /locus_tag="LGJ20_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBK2386175.1"
                     /translation="LISKEGTTEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDI
                     GIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERV
                     GDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTY
                     LKYNPKAKGNGVTIIRQGSN"
BASE COUNT         9233 a        10352 c         9268 g         8593 t
ORIGIN      
        1 ggtgtgatca tgcatctgaa gtcgggtaaa aaactgaaag ctgactgcct gctctatgcc
       61 aacggtcgta ccggtaatac cgattcgctg gcgttacaga acattgggct ggaaactgac
      121 agtcgcggac agctgaaggt caacagcatg tatcagaccg cacagccgca cgtttacgcg
      181 gtgggcgacg tgattggtta tccgagcctg gcatcagcgg cctatgacca ggggcgcatt
      241 gccgcgcagg cgctggtaaa aggcgaagcc accgcgcatc tgattgaaga tatccctacc
      301 ggcatttaca ccatcccgga aatcagctct gtgggcaaaa ccgaacagca gctgaccgcg
      361 atgaaagtgc catatgaagt gggccgcgcc cagtttaaac atctggcacg cgcacaaatc
      421 gtcggcatga aagtgggcac gctgaaaatt ttgttccatc gggaaacaaa agagattctg
      481 ggtattcact gctttggcga gcgcgctgcc gaaattattc atatcggtca ggcgattatg
      541 gaacagaaag gtggcggcaa cactattgag tacttcgtca acaccacctt taactacccg
      601 accatggcgg aagcctatcg ggtagctgcg ttaaacggtt taaaccgcct gttttaacac
      661 tttatcgaaa tggccatcca ttcgtgcgcg gatggcctct gccagctgct catagcggct
      721 gcgcagcggt gagccaggac gataaaccag gccaatagtg cggcgtggtt ccggcttaat
      781 gcacggcaga taaacaaccc catcgcgttt gcgctccggc ggcacagcca gcgcaggcag
      841 taaagtgatc ccgctacctg ccgccaccat gttgcgcagc gtctccaggc tggtcgcgcg
      901 gaagtgtgta tcttcatccg ccccggcttc aaaacagaaa cccattgcct gatcgcgcaa
      961 acagtgacca tcttccagca tcagcagttt ttcccctgcc agatcggcca tcggtacgca
     1021 ttcgcggttc gcccacgggt gatcttcata gatagccagc aacattggct catcaaacaa
     1081 cggcacttca atgaatgctt cgctctcttt caccagcgcg aggatcacgc aatcgagttt
     1141 gccgctgtcc agttgcgcca gtaactggtg ggtctgtgct tcatgcagat acatttccag
     1201 ctttggaaag gtctggtgca gcatagggat aatatgcggt agcaggtacg gtccaactgt
     1261 gggaatcaaa ccaatgtgca gcggcccgga catcgtctcg ccctgctggc ttgccatctc
     1321 tttaaggact ttcacctcac gcagtacggt acgcgcctga tctaccagca gcattcccgc
     1381 ttgggtgaac aacactttac ggctggtccg ctccagcaac atcacgccca actcatcttc
     1441 cagcttacga atttgcccgc taagcgtcgg ctggctaacg tggcaggaat ctgccgcacg
     1501 ccgaaaatgg cggtgttcgg ccaatgccac caggtactca agatcacgaa tattcattat
     1561 ccatcctcca ttgccacgat agttcatggc gataggtaga atagcaatga acgattatcc
     1621 ctatcaagca ttctgactga taattgctca cagaaacgga gcggcacctc ttttaaccct
     1681 tgaagtcact gcccgtttcg agagtttctc aactcgaata actaaagcca acgtgaactt
     1741 ttgcggatct ccaggatccg ctttttttta ttctacaggc ccacagcaag agagcctgat
     1801 agtaacgatt actcaatgcg atctttgaca ataaagaagt aacacagcgc cccggtgaac
     1861 gtcacacagg ccgcgataac caacgccaga ttaaacgagt gtgtggtatc cactacccaa
     1921 ccggttacga tcggagcaaa ggaagcaaaa acaaaactgc caaagttttg aatcccggcg
     1981 atggaagcca cttttgtttc tgacaccatc acctgcacca gcccccatgc agacgttcca
     2041 gcgaaatgca cgcagaatag cgccattgag ataaacgcta ccgcctgggc tggcgaggaa
     2101 gattgcacga ccagcaacgt gcctaatgcc gacatcatca aaccgcagac aatagccgtt
     2161 ttacgcgtct tcgccaggtc gtagcctttt ttcgccagtc gatcgacaac aataccgtta
     2221 acccacatcc cgactgccgc cgcgaggaaa ggaatcgccg ccacccaacc ggttttcgcc
     2281 agactgaaac cttgctcggc ttgcaaataa ccgggtaacc acgcgatgta gagccaaccg
     2341 gtataattga cgccagagaa acctaaaatc atcccccagg ttgtccgatg cttaaacagc
     2401 gccagccact cgctaaattg cagctgtgga cgcggtttaa ccggcgcgga gagatacgtc
     2461 cgttcttcgt cagtcaggac aaactgcgcc cggttgcgat accacgcgta ccaacagatc
     2521 ccaaccagaa tccctgccac gccgatgata acgaacatcg tccgccagcc ccatgccagc
     2581 tgcatcaata caagagcagg aggcgcgata gcctgaccga taacggtaga tgagttaaaa
     2641 atccccagcg ccgtgccgcg ttctttttgt gcataccagt cggtgatcga ctttactccc
     2701 gcaggcataa atggcgcttc gccaataccc agaccgatac gcattaaaat aaagtggcta
     2761 aaagaattaa ccattcctgt taatgcctgc attaatgacc agaatattaa gcctgcgcct
     2821 aaaacaattc gtgggccaaa gcggtctagc aaaataccgg aaggtagttg tgaaaaacca
     2881 taagacagag aaaatgcaga aagcaaaaca ccaaactctg tcgccgataa tcctaactcg
     2941 ccacgaatcg cttcacctgc cacgctcaat gacgaacggt cgagaaaatt aacgatcccc
     3001 gccataaata ataaaaccag ggtcactgtc tgaatacgac gaatccgttg cggtttatta
     3061 cccgcaacac tggttgacac atcaacgtcc atttttagct ccttgagcgg aattaatcga
     3121 tttgatgact attcagaaaa taattaagcg aaccgttatt accagttcca caatgtgcca
     3181 tcttccagtc gtgcgacggg gagatacgcc gggtcatacg gatatttcgc cgccagtttc
     3241 tcatcgaaag aaatacccag ccccggttca tcgcctgggt gcatataacc ttcctcgaag
     3301 cgccagctgt gcgggaaaac ttcaagcatt tgttcggaat agcccatgta ctcctgaacg
     3361 ccaaaatttg gtacccatag gtcaaaatgc agcgccgccg catgacaaac aggcgataaa
     3421 tctgaagggc catgcgaacc ggtacgtacc tgataaagcg atgcaaaatc ggcaatccgc
     3481 cgcatcccgg taatgccgcc tgcatgggtg atcgtcgtgc gaatatagtc aatcagctgc
     3541 tcttcgatta actgcttgca gtcccagata ctgttaaaca cttcaccgac cgcaatcggc
     3601 gtcacggtat gctggcgaat cagacgaaaa cattcctggt tctctgcagg cgtcggatct
     3661 tccatccaga acaggcggta atcctcaatg ctcttcccaa agcgcgccgc ttcgatgggg
     3721 gtcaggcgat ggtgcatgtc atgcaacaga tgttcgttaa aaccgaactt attacgcacc
     3781 gcttcaaata acttcggcgt aaaatcgaga tacttttctg aagaccacaa ctgctcttct
     3841 ggccacaggc ctttggttgc cggttcatac gccaggcctt tgcccttcgc caggccataa
     3901 gtagtttgca tacccggtac accgcactgc acacgaatcg ccttaaagcc catctgctgg
     3961 tgctttgtat agtcttcaag gacttcatca atcgtccgcc cggtggtgtg gcaataaacc
     4021 atcacgcctt cacgagaagc accaccaagc agttgataca atggcatatt tgccgcttta
     4081 gctttgatat cccacaacgc catatcaatg gcagaaatgg ctgacatcgt gacggggcca
     4141 cgtcgccagt aagcaccttt ataaaagaat tgccagatat cttcgatacg gctggcatcg
     4201 cggccaatta gttgtggaca aagatgatct ttcagataag aagcgaccga caattcgcga
     4261 ccatttaacg tggcatctcc taatccacaa agtccgcttt cagtggttat ttgaagcgta
     4321 acgaaattac gccctgggca agtaacaaaa acatctgcag caataatttt cattattgca
     4381 atctcctgtg ttgtaaatag acgcttcaaa cttaatttta cgcctactac catacaagag
     4441 agaaagagcg attattcaac cgtgatcaca ttaaaaataa aaatttccgc cgaggagaaa
     4501 aaataccctc aaaaaccacc tgaataatta actaccatcg ttaaaacaac acgttaaata
     4561 atcacataac aaactccatt aatcattaaa aataagcaca acaatagaaa tgaattatat
     4621 tcaattaatt aaaatatgta ttgattgatg tattgaaaat atgattttta tggggagtgt
     4681 gaaggtaaag aagacacaat gataaaaagg aatgtgacaa ataatggttc tggttgggcg
     4741 ttaaatttaa gctcaccaga atgctgacaa atttctgcct gatgtggcgt aaacgcctta
     4801 tccgcctacg aagtgtcgca ctttgtaggc gtggtaagct ccccattttc aggggagcaa
     4861 tataaacaaa ttactctaac cgcgcctgcg caaaagcaat tgcctgcgcc acctgctgcg
     4921 gtgagacgcc gccttttgcc gcacgcttgt cgaggcacga ttgcagcgac agaatcggat
     4981 agacatcttc gtcaatcacc gggctgaatt tctgcaactc gtcgagcggc agatcttcca
     5041 gcggtttgcc ctgacgaatg gcttccacca ccgcttcacc aacaatatgg tgcgcctcgc
     5101 ggaacggtac gcctttcgct accagataat ccgccagttc ggtggcgttg gcgtaaccct
     5161 gctgagccgc ttcctggcaa cgtggacgtt tcacctgaat gccgtccagc accagcgccg
     5221 ccatatgcag gcagtccagc caggtatcga gcgcgtcgaa cagaccttct ttgtcttcct
     5281 gcatatcttt gttgtaagcc agcggcaaac ctttcagcgt catcatcatg ccggttaacg
     5341 ccccctgcac ccgaccgcat ttaccgcgaa tcagctccag cgcatccggg tttttcttct
     5401 gcggcattaa tgatgaaccg gaagtcacgc ggtcagaaag ctccacaaac cccgcttcgc
     5461 cggtgttaaa gaaaatcaga tcttcagcaa aacgcgacag atgcaccatg ccgatagcgg
     5521 cagccgacag cagttccaac acatggtcac ggtcagaaac gctgtccaga ctgttacggg
     5581 tcgccgaagc aaagcccagc cagcctgcta actgttcacg gtcgatttca taggccgttc
     5641 ccgccagcgc gccacagcct aatgggctga catccagacg cttaagcgca tcctgcaagc
     5701 ggctttcatc acgcgccagc atctcaacat aggccaggca ccagtgcgcg aacgtcaccg
     5761 gctgggcgcg ttgcaggtga gtgtaacctg gcatcaccgc gtcctgattg ttttgtgcgg
     5821 tttccaccag cgccgattgc agctgccggt tagccgtcag caactcgcca acggtatctt
     5881 tgcaccacag tttcaggtct gtcgctacct gatcattacg gctacgcccg gtatgcagct
     5941 ttttgcctaa ctggcccact ttgtcgatca gtttgccttc cacccagcta tggatatctt
     6001 cggcgtcgct ttcaaggatt tgttgtggcc tggcgcgaac atcttccagc aacacgttca
     6061 gcgcctcttc cagttgcgcc tgctcttctg cggttaacac gccgaccgtt accagggctt
     6121 tggaccaggc cacagagcca acaatatcct gctccgccag acggtaatca aagcgcagtg
     6181 agtcgttgaa ttgtttgaac cgttgatctg ctgcctgggt aaaacgcccg ccccaaagtg
     6241 ccataactct gtttccttat tttgaaattc aatgccggaa agctgcgcct ccggccaaca
     6301 aaacttaagc taaaatccgc gtacccatcg gcataccgtt aaacagtgcc ggaagctgct
     6361 ccgcatgacg ccaggaggcg atatctaccg gacggcccag cgtgcgggcc gcatccagcg
     6421 ccgcgttcac tttcactatc atgccgtcag taataatgcc ctgctcaatc agttgttctg
     6481 ctttcgcggc ggtcatttcg gcaatgcgtt gccctttgcc gtcgagaatg ccgctgacgt
     6541 cggagagcaa aatcagatcc gcgcccagcg ttgccgccag cgccgttgcc gcctggtcgg
     6601 cattgacgtt catcagttgc ccttcgtctg ttacgccaat ggagctgacc accggcagat
     6661 aaccgttctc cagcaaggag ttgataagct taggcgaacc tggctgcgcc agtccaacat
     6721 gacctaactc ttcatcaagc tgggtcactt tgacgctgtc gccgtcaccg agaaacaaac
     6781 ctacggccgc aatctgatgt ttcttcgccc atgccaacag ggttttattt gccgttcccg
     6841 ccagtgctcc ggtgataatg tctatctgat cagcaggcgt cacccgcagg ccgtttttct
     6901 ttttcaccgg cagattcagc cctttcatca gctcatccac cacgcaaccg ccgccgtgca
     6961 caatcaccag cggacgctga tgtgactcac gataattcac cagtgcgcta aacagacgtt
     7021 ccagcgcctc ttcactatcc agcagtacgc cgcccagttt gataattaat ggattcatca
     7081 ttgcaccctt aaataagaga ctgcgtttcc gcatagccga aacgaatatt ggcgcactgt
     7141 accgcttgtg ccgccgcgcc tttcagtaag ttgtcttcgg tcgccacaat aatcagatgc
     7201 tcgccctgaa cggcaaaccc gatatcgcaa aatggcagcc caacgacatt tttcagcgcc
     7261 ggaacgcctt tgtcatacag ccgcaccagc ggtttatggg catacgcctg ctgtaacact
     7321 tgcgcgactt gcgcctgggt cacacccgat ttcaggcggc aggtaatggt ttcgagaatg
     7381 ccgcgcggga aattgcccag atgtggggtg aagataacgt cagcaccgag gtgtgtggcg
     7441 atctctggtt gatggcgatg agtaaagacg ccatacggtt gcaggctaac ttcacaaaag
     7501 ctgtttgaaa tggccgcttt acgccctgca ccgctcacgc cgctggtggc gttgatcacc
     7561 ggccactgat tgaggtcaag aagatcggca tcaatcaacg gtttcagcgc cagctgtgcc
     7621 gccgtcggat aacagcccgg caccgcaatc aaattcgctt cttttaattt attaccgcac
     7681 cactccgcca gaccgtaggc tgcctgttcc aacagttccg ggtattgatg ggtaaagccg
     7741 taatattttt catagaaggt ggcgtcgtta acacgaaacg cgccggaaag gtcgaacacc
     7801 acgcagcccg cttcaagaaa ctgcggcgct aaatcgtggc taacttcatg ggcggtggcg
     7861 agaaacacta cgtccacccc tgggctaaac tcgctgatat ccgacatcgg ctgcaacggc
     7921 agatcaacga tgccttttag ctgcggatgc aaatcggaga ttaactttcc cgcatcattg
     7981 ctttgcgctg aaacagtcaa agcggttatg ttcatatgcg gatggcgatt tacataggtc
     8041 actagctctg cgccagcgta gccgctggca cccacaatca gcgtattcaa catcggggct
     8101 attcaccttc ttatgtctgg ttgccaggtt aaacgtaaaa cattcacctt acggctggtg
     8161 ggttttatta cgctcaacgt tagtgtattt ttattcataa atactgcatg aatattgata
     8221 ctatcatgac cagaggtgtg tcaacaatga aaaacaaatt accgccattt atcgagattt
     8281 accgcgctct gattgccaca ccttcaataa gcgccacgga agaggcactc gatcaaagca
     8341 atgcagattt aatcactctg ctggcggact ggtttaaaga tttgggcttc aatgtggaag
     8401 tgcagcctgt tccaggaact cgcaacaaat ttaatatgct ggcaagtatc ggacaggggg
     8461 ctggcggctt gttgctggcg gggcataccg atacggtgcc atttgatgac ggtcgctgga
     8521 cgcgcgatcc gtttacactg acggagcatg acggcaagct ttacggctta ggcaccgccg
     8581 acatgaaagg cttttttgcg tttatccttg atgcgctacg cgatgtcgac gtcacgaaac
     8641 tgaaaaaacc gctctacatt ctggcgactg ctgatgaaga aaccagtatg gccggagcgc
     8701 gttattttgc cgaaactacc gccctgcgcc cggattgcgc catcattggc gaaccgacgt
     8761 cactacaacc ggtacgcgca cataaaggtc atatctctaa cgccatccgt attcagggcc
     8821 agtcggggca ctccagcgat ccagcacgcg gagttaacgc tatcgaacta atgcacgacg
     8881 ccatcgggca tattttgcaa ttgcgcgata acctgaaaga acgttatcac tacgaagcgt
     8941 ttaccgtgcc ataccctacg ctcaacctcg ggcatattca cggtggcgac gcttctaacc
     9001 gtatttgcgc ttgctgtgag ttgcatatgg atattcgtcc gctgcctggc atgacactca
     9061 atgaacttaa tggtttgctc aacgatgcat tggctccggt gagcgaacgc tggccgggtc
     9121 gtctgacggt cgacgagctg catccgccga tccctggcta tgaatgccca ccgaatcatc
     9181 aactggttga agtagttgag aaattgctcg gagcaaagac cgaagtggtg aactactgta
     9241 ccgaagcgcc gtttattcaa acgttatgcc cgacgctggt gttggggcct ggctcaatta
     9301 atcaggctca tcaacctgat gaatatctgg aaacacggtt tatcaagccc acccgcgaac
     9361 tgataaccca ggtaattcac catttttgct ggcattaaaa cgtaggccgg ataaggcgct
     9421 cgcgccgcat ccggcactgt tgtcaaactc cagtgccgca ataatgtcgg atgcgatact
     9481 tgcgcatctt atccgaccta cacctttggt gttacttggg gcgatttttt aacatttcca
     9541 taagttacgc ttatttaaag cgtcgtgaat ttaatgacgt aaattcctgc tatttattcg
     9601 tttgctgaag cgatttcgca gcatttgacg tcaccgcttt tacgtggctt tataaaagac
     9661 gacgaaaagc aaagcccgag catattcgcg ccaatgcgac gtgaaggata cagggctatc
     9721 aaacgataag atggggtgtc tggggtaata tgaacgaaca atattccgca ttgcgtagta
     9781 atgtcagtat gctcggcaaa gtgctgggag aaaccatcaa ggatgcgttg ggagaacaca
     9841 ttcttgaacg cgtagaaact atccgtaagt tgtccaaatc ttcacgcgct ggcaatgatg
     9901 ctaaccgcca ggagttactc accaccttac aaaatttgtc gaacgacgag ctgctgcccg
     9961 ttgcgcgtgc gtttagtcag ttcctgaacc tggccaacac cgccgagcaa taccacagca
    10021 tttcgccgaa aggcgaagct gccagcaacc cggaagtgat cgcccgcacc ctgcgtaaac
    10081 tgaaaaacca gccggaactg agcgaagaca ccatcaaaaa agcagtggaa tcgctgtcgc
    10141 tggaactggt cctcacggct caccctaccg aaattacccg tcgtacactg atccacaaaa
    10201 tggtggaagt gaacgcctgc ttaaaacagc tcgataacaa agatatcgct gactacgaac
    10261 acaaccagct gatgcgtcgc ctgcgccagt taatcgccca gtcatggcat accgatgaaa
    10321 tccgtaagct gcgtccaagc ccggtagatg aagccaaatg gggctttgcc gtagtagaaa
    10381 acagcctgtg gcaaggcgta ccgaattacc tgcgcgaact gaacgaacaa ctggaagaga
    10441 acctcggcta caaactgccc gtcgaatttg ttccggtacg ttttacctcg tggatgggcg
    10501 gtgaccgcga cggcaaccca aacgtcactg ccgatatcac ccgccacgtc ctgctgctca
    10561 gccgttggaa agccaccgat ctgttcctga aagatattca ggtgctggtt tctgaactgt
    10621 cgatggttga agcgacccct gaactgctgg cgctggttgg cgaagaaggt gccgcagaac
    10681 cgtatcgcta tctgatgaaa aacctgcgtt ctcgcctgat ggcgacacag gcatggctgg
    10741 aagcgcgcct gaaaggcgaa gaactgccaa aaccagaagg gctgctgaca caaaacgaag
    10801 aactgtggga accgctctac gcttgctacc agtcacttca ggcgtgtggc atgggtatta
    10861 tcgccaacgg cgatctgctc gacaccctgc gccgcgtgaa atgtttcggc gtaccgctgg
    10921 tccgtattga tatccgtcag gagagcaccc gccataccga agcgctgggc gagctgaccc
    10981 gctacctcgg tatcggcgac tacgaaagct ggtcagaggc cgacaaacag gcgttcctga
    11041 tccgcgaact gaactccaaa cgtccgcttc tgccgcgcaa ctggcaacca agcgccgaaa
    11101 cgcgcgaagt gctcgatacc tgccaggtga ttgccgaagc gccgcaaggc tccattgccg
    11161 cctacgtgat ctcgatggcg aaaacgccgt ccgacgtact ggctgtccac ctgctgctga
    11221 aagaagcggg tatcgggttt gcgatgccgg ttgctccgct gtttgaaacc ctcgatgatc
    11281 tgaacaacgc caacgatgtc atgacccagc tgctcaatat tgactggtat cgtggcctga
    11341 ttcagggcaa acagatggtg atgattggct attccgactc agcaaaagat gcgggagtga
    11401 tggcagcttc ctgggcgcaa tatcaggcac aggatgcatt aatcaaaacc tgcgaaaaag
    11461 cgggtattga gctgacgttg ttccacggtc gcggcggttc cattggtcgc ggcggcgcac
    11521 ctgctcatgc ggcgctgctg tcacaaccgc caggaagcct gaaaggtggc ctgcgcgtga
    11581 ctgaacaggg cgagatgatc cgctttaaat atggtctgcc agaaatcacc gtcagcagcc
    11641 tgtcgctgta caccggggcg attctggaag ccaacctgct gccaccacct gagccgaaag
    11701 agagctggcg tcgcattatg gatgaactgt cagtcatctc ctgcgatctc taccgtggct
    11761 acgtacgtga aaacaaagat tttgtgcctt acttccgctc cgctacgccg gaacaagaat
    11821 tgggcaaact gccgttgggt tcacgtccgg cgaaacgtcg cccaaccggc ggcgtcgagt
    11881 cgctgcgcgc cataccgtgg atttttgcct ggacgcaaaa ccgcctgatg ctccccgcct
    11941 ggctgggtgc aggtacggcg ctgcaaaaag tggtcgaaga tggcaaacag agcgagctgg
    12001 aagccatgtg ccgcgattgg ccattcttct cgacgcgtct cggcatgctg gagatggtct
    12061 tcgccaaagc agacctgtgg ctggcggaat actatgacca acgcctggta gacaaagcac
    12121 tgtggccgtt aggtaaagag ttacgcaacc tgcaagaaga agacatcaaa gtggtgctgg
    12181 cgattgccaa cgattcccat ctaatggccg atctgccgtg gattgcagag tctattcagc
    12241 tacggaatat ttacaccgac ccgctgaacg tattgcaggc cgagttactg caccgctccc
    12301 gccaggcaga aaaagaaggc caggaaccgg atcctcgcgt cgaacaagcg ttaatggtca
    12361 ctattgccgg gattgcggca ggtatgcgta ataccggcta atcttcctct tctgcaaacc
    12421 ctcgtgcttt tgcgcgaggg ttttctgaaa tacttctgtt ctaacaccct cgttttcaat
    12481 atatttctgt ctgcatttta ttcaaattct gaatatacct tcagatatcc ttaaggaatt
    12541 gtcgttacat tcggcgatat ttttttcaag acaggttctt actatgcatt ccacagaagt
    12601 ccaggctaaa ccccttttta gctggaaagc cctgggttgg gcactgctct acttttggtt
    12661 tttctctact ctgctacagg ccattattta catcagtggt tatagtggca ctaacggcat
    12721 tcgcgactcg ctgttattca gttcgctgtg gttgatcccg gtattcctct ttccgaagcg
    12781 gattaaaatt attgccgcag taatcggcgt ggtgctatgg gcggcctctc tggcggcgct
    12841 gtgctactac gtcatctacg gtcaggagtt ctcgcagagc gttctgtttg tgatgttcga
    12901 aaccaacacc aacgaagcca gcgagtattt aagccagtat ttcagcctga aaattgtgct
    12961 tatcgcgctg gcctatacgg caatggcagt tctgctgtgg acacgtctgc gcccggtcta
    13021 tattccaaag ccgtggcgtt atattgtctc ttttgccctg ctttatggct tgattctgca
    13081 tccgatcgcc atgaatacgt ttatcaaaaa caagccgttt gagaaaacgt tggataacct
    13141 ggcctcgcgt atggagcctg ccgcaccgtg gcaattcctg accggctatt atcagtatcg
    13201 tcagcaacta aactcgctaa caaagttact gaatgaaaat aatgccttgc cgccactggc
    13261 taatttcaaa gatgaatcgg gtaacgaacc gcgcacttta gtgctggtga ttggcgagtc
    13321 gacccagcgc ggacgcatga gtctgtacgg ttatccgcgt gaaaccacgc cggagctgga
    13381 tgcgctgcat aaaaccgatc cgaatctgac cgtgtttaat aacgtagtta cgtctcgtcc
    13441 gtacaccatt gaaatcctgc aacaggcgct gacctttgcc aatgaaaaga acccggatct
    13501 gtatctgacg cagccgtcgc tgatgaacat gatgaaacag gcgggttata aaaccttctg
    13561 gatcaccaac cagcagacga tgaccgcccg caataccatg ctgacggtat tttcgcgcca
    13621 gaccgacaag cagtactaca tgaaccagca acgtacgcag agtgcgcgtg aatacgacac
    13681 caacgtgctg aagccgttcc aggatgtgct gaatgaccct gcgccgaaga aactgatcat
    13741 cgttcatctg ctgggtacgc atatcaaata caaataccgc tacccggaaa atcagggcaa
    13801 gtttgatggc aataccgatc atgttccgcc aggattaagc gcagaagagc tggaatcata
    13861 taacgattat gacaacgcta acctgtataa cgatcatgtg gttgccagcc tgattaaaga
    13921 ctttaaagcg gcagacccga acggattcct ggtttacttc tctgaccacg gtgaagaggt
    13981 ttacgacacg ccgccgcaca aaactcaggg gcgtaacgaa gacaacccga cgcgccacat
    14041 gtacaccatt ccgttcctgc tgtggacgtc ggaaaaatgg caagcgactc atccccgtga
    14101 tttctcacag gatgtcgatc gtaaatacag cctggcggaa ctgatccaca cctggtcaga
    14161 tttggcgggc ttatcttacg acggttacga tccaacccgt tcagtggtga atccgcagtt
    14221 caaagaaact acccgctgga ttggtaaccc gtacaagaaa aacgcgctga tcgattacga
    14281 cactctgccg tatggcgacc aggtaggtaa tcagtaaatg acctttgtat cgcgtctggc
    14341 gctatttgcc ggatgcgata ctgccgcaat gtttacatcg aactcccctc aggccacact
    14401 tttcaggtgg ccttttttaa taatgccagt tactgttttc ccgtctacat acctaaggga
    14461 ataacatgta tcacgacgtc agctatctgc tctcccgcct gatcaacggc ccgctgtccc
    14521 tgcgccagat ttactttgcc agcagtaacg gtccggcgcc cgatcttgcg tatcaggtcg
    14581 attttccacg gctggaaata gtgctggaag gtgagtttgt tgataccggc gctggagcaa
    14641 cgttagttcc cggcgatgtg ctgtacgttc ccgctggtgg ctggaatttt ccacaatggc
    14701 aagcccccgc tactaccttt agcgtgctgt ttggcaaaca gcaactcggc ttcagcgtcg
    14761 tgcaatggga tggcaaacaa tatcaaaatc tggcgaagca acacgtcgcc cgacgcggcc
    14821 cacgtatagg ttcttttctg ctacaaacgc tcaatgaaat gcaaatgcag ccgcaggagc
    14881 agcaaacggc aaggcttatc gtagccagcc tgcttagcca ctgccgcgat ttgctcggca
    14941 gccaaatcca gaccgcctca cgcagccagg cactatttga agctattcgc gattatatcg
    15001 acgaacgcta tgcctccgcg cttacccgcg aatctgtcgc acaggcattc tatatttcac
    15061 caaactatct ctcgcacctg tttcaaaaaa cgggggccat tggttttaac gaatacctga
    15121 atcacacgcg actggagcac gctaagacgt tattgaaagg ctatgacctg aaggtaaaag
    15181 aagtggcgca cgcctgcggc tttgtcgaca gcaactactt ctgccgtctg tttcgtaaaa
    15241 acaccgaacg ctcgccgtcg gaatatcgcc ggcagtacca cagccaactg acagaaaaac
    15301 cgactactcc agaataagat gggtttgctg cggcgcagaa agcactttgc gcaccgcgct
    15361 cattacccgc tgcggctcac gcaggaatgc gtagatgctg cattgcacat agcggcaatg
    15421 ttcaaatcgc tcggcaccgg caagctcgat atccgtaatc aacagagcaa catccgcccg
    15481 acggatatcc tcgtccgcta aacgattctc cgttcccagc gcgccctgag tttcaatgct
    15541 gacgccccac ttctctaact ggcacaactt ttccagccgt tccgccgcca tataagtatg
    15601 cgccacgccg cttacgcagg cggttactgc caccaggtat gccattttaa cctcccacgg
    15661 taacctgaaa tccggcccgt tctgccattt cgcgcatcgt tgtcacatcg gctgacgacg
    15721 gcgcaggcac ctctttcatc gaccatgttt tccccagcag gcggtatttc ggttcgccgt
    15781 actgatgaaa cggtaacaga tggatctgcc tgattttcag cgggatcagt acatccagcg
    15841 cctgctgcat attctcccgg ctgagcgtga aaccagggat caacggtaaa cgcgggatca
    15901 cgttgacacc ctcactcacc agcaaacgca gattctccag cacgcgtggc aggttcatct
    15961 tcaccacatc ccgcgcctga gtcgcgtcca taatttttaa atcgaacagc acttcatcgc
    16021 acaatttcgc cagtggtaat agcttgctgg ctggcgcgtc tccggcagtt tcaatggcgc
    16081 atgacacgcc ccacaaccgc agccgctgta aaaaacgggt agcaaactcc gcctgcatta
    16141 acacttcgcc gccagaaagc gtgacgccgc cgccggacgt gcgaaagaaa atgtcatctt
    16201 tcatcacttc ccgttccagc gcgtcaaggc tgatatcgcg gccaatccgt tcaaacgccc
    16261 cggaggggca ttcatccgca tcacgcaaac atttcgcaca gtgcagacat ttcgcctctc
    16321 tgcgtaccgt ctggattttg ccggagatcg actccggatt agcacaccac gggcaaagat
    16381 gcggacagcc tttaaaaaag accaccgtac gaatgccctc gccgtcattc agtgaataac
    16441 gctgaatgtt gaaaatgcgc gccacatcat cgcggcgcgt ttccacaacg ttacagctga
    16501 tgcgctgtcc ggcggatgat gtcatcctgg atctccttcg acagttcgac aaagaaggcg
    16561 ctgtatccgg caacgcgcac caccagcccg gcataatctt gtgggcgctg ttgcgcttcc
    16621 cgcaacgtgt cggcgttcac cacgttaaac tgaatatgct gcaacttaag ctgggtaaac
    16681 gcccgtaaga agtcggccag tttgcgtaat cccgcttcac cttccagggt cgccggagtg
    16741 aatttcacgt tcagcaacgt accgttagac agcagcgtgt tatcgagctt actgactgac
    16801 ttcagtaccg ctgttggccc ttgtgcgtcc tggcccagca taggtgacaa gccgccgtct
    16861 gccagttgtt ccccggcaaa acgaccgtct ggcgtcgcgc caaccaccga tcccaacggg
    16921 acgtgagcag aaacggtata cgatcccggc gtgaagtaac cgccgcgcgg gttctggtat
    16981 ttttccactt ctttgcagta gtggcgtaac agttcggcgc tgatgttatc cacctcgtcg
    17041 atatcgttac cgtatttctc gaagcggtta attaagcgag cacggacttt ttcgccttct
    17101 ggcgttgcaa agttggcttt taataccgac agcaattcgt caaaacttaa acgctgttgc
    17161 tcaaaaacca tccctttcaa cgcatggaga gaatcgctca ggttggcgat accgatcccc
    17221 tgtacgccgg agaagttata acgcgcgccg ccatcggtaa tatcgcggcc tttttccaga
    17281 caatcgctga taaaagacga gagcagcggt acaggtgccc agtcgcgatg gccgatatca
    17341 caaatattgc tgccctcaac catcagggtg atgtagtggc tgatcttggc gcggatctgc
    17401 tccagtaaac cttcataagt cagctcagca ttgccttcat tttcatacag gcagatttcc
    17461 atcactttca gcagattaaa catcgcgatg tcatgcaagc cgtaggttct gccgggaata
    17521 gataattcca cacagcccac tacggaatag tcgcgcgcat cttccagcga aacgccacgg
    17581 ttgaggaacg ctggcaccac cacttcatcg ttaaagattt gcggaatacc ggtgccgagg
    17641 cgaatggttt cggcggtttt catcaggaac ggcgtgtcga tcaaggcgtt agtgcgcacg
    17701 ccgaggttcg gttgcggtaa ttgcacgctt tgataggcgt caaggcaaag gaacgaaagc
    17761 acgttcaccg cgctacgtcc gttctcggtt aacccgccga gcagtgcggt atagccggtc
    17821 gggaaaccgg cgaaataacg cgcgctactg gtggagcgca acagcacgat gtcgttgcat
    17881 ttcacccata aagattcgag tagttctttc aggaacgccg gatcttcgcc ctgggttaaa
    17941 gatgcctgat agaacggcaa catatactgg tcgaagcgcc ccaacgatag cgaactggcg
    18001 ttggattcgt attgcagaat gatgttcatg taccagaata actggcacgc ctgccagaac
    18061 gtctgcggct tatgttcggc gttatggcgg gagatctccg caatagtcag cagttcttcg
    18121 cgacgctggg catctgtgca gtttgccgcc attgtttccg ccagttcagc gtaacgcaga
    18181 atgtgtttct gcgaggcttc cagcagtaac agcgcggcct gataaaagtg attctccggc
    18241 tgttgctgac aatgttgctg catctgcgcc accagctccc ccagcccgtg attcagcagg
    18301 cgtgggtaat caataataat gtgcccctgg cctttatctg tctggttgat gctgaaaatc
    18361 tgcgtgctgg tcgcggcttt tacttcatct gtcatctgcc cgttgatgaa atctttcatc
    18421 gaacgttttt cccagtacgg gaacaactct tcacggtaga tacgtttatc ttcttcactg
    18481 atagcaaagc ggtcctgcgg acgcgtcggg aattgatcca gctctttcag cagccagtaa
    18541 gggtccattt ccggcgacat aatcccagcg cgcggtttta cggtgcggtt accggcaatc
    18601 agttcttcat cacgaatcga aatttcaaca tgttcaagga tatacgctgt tgctttcgcc
    18661 cggcgcaata tcaccggttc gccttcggtt tgccgatggc tggcggtata aagcagcgcc
    18721 cgctccagcg agatttcacg ggtattggca aacagtgcag ttttgaggcg agagatacga
    18781 ttcgtcatga ggaactcctg tgggaaagcg aacccgccct tgcgagcggg agaagaactt
    18841 aagcggtttg cgccaggtga gcttcaattt tgctcataat ggcgtcagca cgttttactg
    18901 catcgctgat gttgacgcgc acgatggtct tgccagcaaa acgctcttca aacttgatgc
    18961 cgatatcttt ggtcagaatc accatatcag cgctcgccac atcttccgca gtcagttcat
    19021 tttccagacc aattgacccc tgggtttcta ctttcacttc ccagcctttc gctttcgcgg
    19081 cactttccag cgcctctgct gccatatagg tatgggcaac acctgaagga catgcggtta
    19141 ctgcaataat tttcgtcatg gctcgttcct caattaatta atttcaaaat ccaaatccag
    19201 gtcgtcttct ttttcatcag tcgaagaccc atttttacgc gccagacttt tcagcacgtt
    19261 cacacaaaca gccgtcacca ctgctccgac agccactgct gcgatataac ccagcttgcc
    19321 ttcaaccacc ggcagcacaa tcagaccacc ccagcctgca tagcactgcg caccgaccag
    19381 cgccgcagtt actgcaccac aaactgaacc gaccatgatg gaaggaatga cacgcagcgg
    19441 atcggcagcg gcgaaaggaa tcgccccttc agtaacgcca acacagccca tcaccagtgc
    19501 cgctttgccg gtttcgcgct cttctgcgga gaaattttta cgaccaatca gcgtcgccag
    19561 ccccatcccc agcggcggga tacaaatacc cacggcagcg atggccacca cggtataaac
    19621 accctgagca acgcaaatca gcatgaaggc ataggccact ttgttaaccg gaccgcccat
    19681 atcgaacgcc agcatcagac ccatgatcac cgccagcata acaatgctgc cctgctgcat
    19741 cccctgaagc cactgagtca ggctgttggt caacgcccct acaggctcgc ccaagcccca
    19801 catcatgatg cctgcggtaa tcagtgtgcc gacgatagga ataatgaaga taggcatcac
    19861 cgagcgcaga actttatgca ccggaatttt cttcaggtaa tgcaccacga tgccgccgat
    19921 aatcccggcg atcagtgcac caaagaagcc cgcaccaaag ctgttaccaa cccaggcacc
    19981 gatagcgcac ggagccagcg cagaacgctc tgcaatggag taaccgatgt aagcggcgag
    20041 gaaaggcacc atcagcgtca aacctgcaac gccgatatca aacagttttt tcagattcgg
    20101 atcggctacg gcatccggca ctgcgccttt gccatacaac atgacggaaa ccgccagcaa
    20161 aataccgccc gataccacga agggaatcat gtgtgaaacg cccgtcatta aatgctgacg
    20221 ggtatttttc aggatctgca ccaactcatt cataagctgc tcccgggctt gcgaaagccc
    20281 atgtccatat ttttgatgga ggaaacaata ggcaatcgca ggcggagcaa acagtggata
    20341 aaaaggccat ttactggact tttgtaatat cagtacaaaa atgcgatccg cctcataact
    20401 tgcgataaac cggaagcatg ccgcaaaaca gcgtcttgtg agacgcgtcg caaattgacg
    20461 gttacattac atttgtccag cttttggcag atttgtcaca tggcaggccg ttgccgtgcg
    20521 ctcttatcct gtaaacagaa aaccgtaccg ggagagaggc aatggccctg attgtggaat
    20581 ttatttgtga gctacctaac ggcgtacatg cacgtccggc aagccacgtt gaaacgctgt
    20641 gtaatacttt ttcatcacaa attgagtggc ataacctgcg cactgaccgc aagggcaacg
    20701 ccaaaagcgc ccttgcgctg attggcaccg atacgctggc gggcgataac tgccagttac
    20761 tgatttccgg ggccgacgaa caggaagcgc accagcgttt aagccaatgg ctgcgcgatg
    20821 aattccccca ctgcgatgcg ccgctggcgg aagttaaatc tgacgaactg gaaccactgc
    20881 cggtttcact gaccaatctg aatccgcaaa ttatccgcgc ccgcaccgtg tgcagcggta
    20941 gtgcaggcgg cattctgacg ccgatctctt ctttagatct caatgcgctg ggtaatcttc
    21001 ccgcagccaa aggcgttgac gccgagcaat ccgcactgga aaacggcctg acgctggtac
    21061 tgaaaaacat tgagtttcgt ctgctggata gcgacggtgc caccagcgcg attctggaag
    21121 cgcaccgatc cctggctggc gatacttccc tgcgcgaaca tttactggca ggcgtcagcg
    21181 ccggattaag ctgcgccgaa gcgattgtca ccagtgcgaa tcacttttgc gaagagttcg
    21241 cccgttccag cagcagctac ctgcaagaac gtgccctgga cgtacgcgac gtctgcttcc
    21301 agttactcca gcaaatctac ggtgagcaac gcttcccggc accgggcaaa ctgacgcagc
    21361 ccgccatttg tatggctgat gaactgactc ccagccagtt cctcgaactg gataaaaatc
    21421 acctcaaagg tctgttactg aaaagtggcg gcaccacctc acataccgta attcttgccc
    21481 gttcgttcaa cattccgaca ctggttggtg tagatattga tgcccttact ccgtggcagc
    21541 atcaaacgat ttatatcgac ggcaacgctg gggcgattgt ggttgagcca ggagaagccg
    21601 tagctcgtta ttatcagcaa gaagcccgcg tacaggacgc cctgcgtgag caacagcgtg
    21661 tctggctgac ccaacaagcc cgtaccgctg acggtatccg cattgaaatt gccgctaaca
    21721 tcgctcactc cgtggaagcg caggccgcat tcggcaatgg tgcggaaggc gttggtttgt
    21781 tccgcactga aatgctctat atggatcgca ccagtgcacc gggcgaaagc gagctgtaca
    21841 acattttttg tcaggcgctg gagtctgcca acggacgcag cattattgtg cgcactatgg
    21901 acattggcgg cgacaaaccc gttgattatc tgaacattcc ggcagaagct aacccgttcc
    21961 tcggttatcg cgctgtgcgt atttatgaag aatacgcctc gctgttcacc acacaactac
    22021 ggtcgatcct gcgcgcctcc gctcacggca gcctgaaaat catgatcccg atgatctcct
    22081 caatggaaga gatcttatgg gtgaaagaaa aactggcgga agccaaacag caactgcgta
    22141 acgaacacat tccgtttgat gagaagatcc agcttggcat tatgctggaa gtaccgtcgg
    22201 tgatgttcat catcgatcaa tgctgcgaag agattgattt ctttagtatt ggtagtaatg
    22261 acctgacgca atatctgctg gcagtggatc gcgataacgc taaggttact cgtcactaca
    22321 acagcctgaa tccggcattc ttgcgggcgc tcgattacgc cgtgcaggcg gtgcatcgcc
    22381 agggcaaatg gattggtctg tgcggtgagc tgggagcgaa aggttccgtg ctgccgttgc
    22441 tggtcggctt agggctggat gaactcagca tgagcgcacc atcaattccg gcggcgaaag
    22501 cgcggatggc gcaacttgat agccgtgagt gccgcaagtt gctcaaccag gcaatggcct
    22561 gccgtacttc gctggaagtg gaacacctgc tggcgcaatt ccgcatgacc caacaggacg
    22621 caccgctggt caccgccgag tgcatcacgc tggaaagtga ctggcgcagc aaagaagaag
    22681 tgctcaaagg catgaccgat aacctgctgc tggcgggccg ctgccgctat ccgcgtaaac
    22741 tggaagccga cttatgggcg cgcgaggccg ttttctctac cggtctgggc tttagttttg
    22801 ccattccaca cagcaaatca gaacacattg agcaatccac catcagcgtg gcgcgtctgc
    22861 aagcgccggt gcgctggggc gatgatgaag cgcaattcat cattatgtta accctgaaca
    22921 aacacgctgc gggcgatcag cacatgcgca ttttctcgcg cctcgctcgc cgcatcatgc
    22981 acgaagaatt ccgtaacgcg ctggttaacg ccgcctctgc cgacgctatc gccagcctgc
    23041 tgcaacatga actggaactg taaaaggaaa catcatggaa ctgtatctgg acaccgctaa
    23101 cgtcgcagaa gtcgaacgtc tggcacgcat attccccatt gccggggtga caactaaccc
    23161 gagcattatc gctgccagca aggagtccat atgggaagtt ctgccgcgtc tgcaaaaagc
    23221 gattggtgat gagggcattc tgtttgctca gactatgagt cgtgacgcgc agggtatggt
    23281 ggaagaagcg aagcgcctgc gcgacgccat tccgggcatt gtggtgaaaa tcccggtgac
    23341 ttccgaaggt ctggccgcca ttaagatgct gaaaaaagag ggtattacta cgcttggcac
    23401 cgcagtgtac agtgctgcac aagggttact ggccgcgctg gcaggggcaa aatacgttgc
    23461 tccgtatgtt aaccgcgtag atgcccaggg cggggacggc attcgtacgg ttcaggagct
    23521 gcaagcgctg ttagaaatgc acgcaccaga aagcatggtg ctggcggcca gctttaaaac
    23581 gccgcgtcag gcgctggact gtttactggc aggatgtgaa tccatcaccc tgcccttaga
    23641 tgtagcgcaa caaatgctca acacccctgc ggtagagtca gctatagaga agttcgaaca
    23701 cgactggaat gccgcatttg gcactactca tctctaaagg agcaattatg gaccgcatta
    23761 ttcaatcacc gggtaaatac atccagggcg ctgatgtgat taatcgtctg ggcgaatacc
    23821 tgaagccgct ggcagaacgc tggttagtgg tgggtgacaa atttgtttta ggttttgctc
    23881 aatccactgt cgagaaaagc tttaaagatg ctggactggt agtagaaatt gcgccgtttg
    23941 gcggtgaatg ttcgcaaaat gagatcgacc gtctgcgtgg catcgcggag actgcgcagt
    24001 gtggcgcaat tctcggtatc ggtggcggaa aaaccctcga tactgccaaa gcactggcac
    24061 atttcatggg tgttccggta gcgatcgcac cgactatcgc ctctaccgat gcaccgtgca
    24121 gcgcattgtc tgttatctac accgatgagg gtgagtttga ccgctatctg ctgttgccaa
    24181 ataacccgaa tatggtcatt gtcgacacca aaatcgtcgc gggcgcacct gcacgtctgt
    24241 tagcagcggg tatcggcgat gcgctggcaa cctggtttga agcgcgtgct tgctctcgta
    24301 gcggcgcgac caccatggcg ggcggcaagt gcacccaggc ggcgctggca ctggctgaac
    24361 tgtgctacaa caccctgctg gaagaaggcg aaaaagcgat gcttgccgcc gaacagcatg
    24421 tagtaacccc ggcgctggag cgcgtgattg aagcgaacac ctatttgagc ggcgttggtt
    24481 ttgaaagcgg cgggctggcg gcggctcacg cggtgcataa cggcctgacc gctatcccgg
    24541 acgcgcatca ctattatcac ggtgaaaaag tggcatttgg tacgctgacg cagctggttc
    24601 tggaaaacgc accggttgag gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt
    24661 tgccaataac tctcgctcaa ctggatatta aagaagatgt cccggcgaaa atgcgaattg
    24721 tggcagaagc ggcatgtgca gaaggtgaaa ccattcacaa catgcctggc ggcgcgacgc
    24781 cagatcaggt ttacgccgct ctgctggtag ccgaccagta cggtcagcgt ttcctgcaag
    24841 agtgggaata acctactcca aactcccggc ttgtccggga gtttgaacgc aaaattgcct
    24901 gatgcgctac gtttatcaag cctacgaaat ctctgcaata tattgaatat gcgtgctttt
    24961 gtaggccgga taaggcgttc actccgcatc cggcactctg tgccagtaat ctgaaactcc
    25021 cacgttgttc aggagttttt tatccgcttc tggacatttt ctccacagaa caatcgttag
    25081 cccggaaggt cgaatctgtg actcttattt tcacggacga accagatgaa agcttcactg
    25141 gcgctgctca gcctgctaac ggcatttacc agccactcct taaaatctcc cgccgttcca
    25201 ccaaccgtgg tacaaatcca ggccaatacc aaccttgcca tcgcagacgg tgccagacag
    25261 caaatcggca gcacgctatt ttacgatccg gcgtatatgc agcttaccta tcctggcggt
    25321 gatgtcccgc aagaacgcgg ggtatgttcc gatgtggtga tccgtgcatt gcgcagccag
    25381 aaggtcgatt tgcagaaact ggtgcacgaa gatatggcga agaattttgc cgagtacccg
    25441 caaaaatggc agttaaagcg cccggacagc aacatcgatc accgtcgggt gcctaatctg
    25501 gaaacctggt ttacccgcca cgataaaaca cgccccatca gcaagaaccc cagtgactat
    25561 caagcgggcg atattgtctc ctggcgactg gataacggac tggcacatat tggggtggtg
    25621 tcagatggct tcgcccgcga cggtacgccg ctggtgatac ataacattgg cgcaggcgcg
    25681 caggaagaag atgtgctgtt tagctggcgg atggtggggc attatcgtta ttttgttaaa
    25741 taagcgatcg ccccacatga agaaaaaatt cagatgcttt ttacactgac agcttctttt
    25801 ttcttacggc tcaccagcgc cagtgccagc acaatcagca caataccgct accttcaacg
    25861 gcccccggat tctcaccgag cagccaccag gaaaagagta cgccgcaaac gggaacggcc
    25921 agcgtgctta agctggcaat actggcaggc aagtttttca atacaaacaa ccataagctc
    25981 cacgccagcg ccgtcgccag aatcgcactg taggccagcg cccagaacac ggtgggctgc
    26041 cagtcaattt cacgttgcgg caccagtaaa gcgaccacac tcatcaccag cgccgcatag
    26101 agcatctgcc aggatgttaa cgacaataaa tccacgcgcg gatgacgggc atacagacgt
    26161 ttagcaacaa tcgcgctcgc cccccaactg acgccggaga ggattgccag catggcactt
    26221 ttcatcgaag agaaatcgag ttgccacggc tgcaacacca aaaataagcc gaaagcggca
    26281 atcagtatcg cgaaatattg cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa
    26341 atcaccaccc agaacggcat ggtatagctt aggatcgcca ccttccccgc tccgccgctg
    26401 accagcgccc actgcgccag gccaaccatc ccgcaggttt gtaacagggc aatggctaag
    26461 gtgtatttaa acggtgtcgg gcgcattccg cgaccacgta ataaaaggac gatgaataaa
    26521 acgagagcgc cgaaaatgca gcgtaaggcg gtaaagtcga aggcaccgat gtagctggtg
    26581 acttgcttca tgaaaatcca gctataactc cagataagtg tgagcacaac caggccactg
    26641 attgccagtg ggttgctctt tcctgcgaca gacatgagag atccagtgtg tagttttcct
    26701 gtggacacta tacgccggta aagaaacttc agcgactgcc tgaaaggcag tcgctgaaga
    26761 aggtcagatt acagcaggtc gaaacggtcg aggttcatca ctttcaccca tgccgccacg
    26821 aagtctttaa caaacttctc gtgagcatcg ctgctggcgt aaacttcagc caccgcacgc
    26881 aggacagagt tagaaccaaa taccagatcc gcacggctgg cagtgtattt cacttcgccg
    26941 gtttcgcggt cacggccttc aaacagctct ttcgattcgt cggtcgcttt ccactcgtaa
    27001 cgcatatcca gcaagttcac gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag
    27061 acgccgtttt tgctgccatc gaagttggca cccagtacac gcatcccgcc caccagcgca
    27121 gtcatttccg gcgcggtcag cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg
    27181 gtggaaacgt ccagacgagc gcgatagtta cggaaaccgt cagcaattgg ctccagcagc
    27241 tcaaacatct caatgtcagt ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt
    27301 acatgaatgc tcaaacctgc ggcgcttgcg gctttctcaa caccaaccac accagccagc
    27361 acgatgatat ccgccagcga ggctttacca gactctttct ggattttctc cagaacaggc
    27421 agagcacgaa cggctgcggc gttcacatcc cagtcgcgct gcggcattaa tgccagacgc
    27481 gcaccgttgg caccaccgcg tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc
    27541 accgatacca gctcactaac agacagacca gaatccgcaa tcgcgaattt cagatcgata
    27601 atgtcctgct cggtcgggtt gtagatcggc tgcggcagcg gatcttgcca gatcagatct
    27661 tctttcggta cttccggtcc gatgtaacga gatttcggcc ccatatccct gtgcgtcagt
    27721 ttgaaccagg cacgggcaaa ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga
    27781 gagatcttct cgaactcagg atcaaaacgc agcgtcaggt cggtcaccag catcgtcggt
    27841 ttacgtttct tcgacggatc gaacggatcc gggataattt ccggtgcgtc taccgcttcg
    27901 aactggattg cgccagccgg actgcgggtc tgtacccact catatttgaa caggttctcg
    27961 aagaaatagt tgctccactg ggtcggcgtc tgggtccaga caacttccag accagaggta
    28021 atggcatctg cgccaacgcc gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca
    28081 atcggtgcag cttctggatc aggacctaca tttgatgtcg gaccggcacc gtgggtttta
    28141 cccagcgtat gaccacccgc aatcagcgcc acggtttctt cgtcgttcat gcccatgttg
    28201 ccgaaggtcg cgcggatagc tgctgccgca gaaagcggtt cgccgctgtg gtccgggcct
    28261 tccgggttaa cgtaaatcag gcccatctcg gttgcaccca gcggtgcttt cgccagcgct
    28321 tccggatgac ggtgagtcag ccaggctttt tcatcacccc agttaacatc cagatccggt
    28381 tcccagacgt cttcacgacc ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc
    28441 gccacgttac ctgcgaggat aaacaggtcg gcccaggaga ttttctgacc atatttctgt
    28501 ttgattggcc acaacaggcg acgcgcttta tcgaggctta cgttatccgg ccaagagttc
    28561 agcggtgcaa aacgttgctg accacgaccc gcgccgccgc gtccatcgat tgaacggtaa
    28621 gtacccgcgc cgtgccaggc catacgaata aacagaccgg cgtaactgcc ccagtcggca
    28681 ggccaccacg gctgagattc tgtcagcagg gctttcagat cttttttcag gccgtagtaa
    28741 tctaatttgc tgaattcttt gcggtagtca aagtcctcac ccagtgggtt agaacgatta
    28801 gaatgttggt ttaacaggtc aacacggagt tgatttggcc accagtcgcg agtggttgtg
    28861 cccgcccccg cgctctggtc gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg
    28921 gtgttatgaa tatcgtctga cgtgctcatc aatgtgctcc cctctacagt gttaccgtta
    28981 cgatacacag cgttagagag aagttataat tgaattaatc cacggatgcg atagttgaga
    29041 tcttacatat tgttggttaa agagatgtag atcaaattga tcgtaattaa gcttttttga
    29101 ttatgtccga attcggacaa atgctttata aaaaagggtt ttaggttcat atccttcaag
    29161 taatgaggga gaaaacaagg ctgataacct tattttcccg ccctcatttc gaggcagcat
    29221 tttgtgctct gtttaaaatt tgtgatcact gtgtgatttt cacaaaagcc acactattta
    29281 taaaccaggt cgaacaccca gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag
    29341 cgtatagaag tggaaatctt tcactccttc acggcttaaa atcttcacca tatccatggc
    29401 aatattcgct cccaccagtt tgcgggtttc ggcatcgtcg tccagcccgt caaacatttg
    29461 tgccatccac gccggaatac gcacgttggt catatcggca aatttcttcg cttgtttaaa
    29521 gttcgagacc ggcaaaattc ccggaataat ttccacatca atacccgccg atacgcagcg
    29581 gtcacgaaaa cgcaggtagc tttcgacatc aaagaagaac tgagtaatcg cgcggttggc
    29641 tccggcatcg actttgcgtt tcaggttgag caaatccgcc tgagcgcttt ttgcttccgg
    29701 gtgaacctcc ggatacgccg ccacggagat atcgaaatct gccacttctt ttaacagcgt
    29761 caccaggtca gaagcataca tttccggctt accacttccc ggcggcagat cgccacgcag
    29821 cgccacgata tgacgaatac cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc
    29881 gggcgtcgca tcaatgcaag taagatgcgg tgccgcttcc agaccagtgc gatctttaat
    29941 gcctttaata atgctgtgcg tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga
    30001 tacaaacttc ggtttcaggc tgctaaggcg atcgatggag ttccacaggg tctgctccat
    30061 ttcactggta cgcggcggga aaaactcgaa cgaaacgtta atttgcccct ggacttctgc
    30121 caggctctga ttcagggcat cccgctggct ggcgtgaaaa aagctcatac cttacctcat
    30181 caatcgcttg tagttatatg ttgtgttgtg cacatctatc cgtttagacg tccagatgta
    30241 aagatgaagg aaaagccgaa gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa
    30301 tcagggaaga tgaaaaaact tcaggatggt aagaaaaaga aaatccggta tgcaaagcaa
    30361 accggactgc aaatataaac taaccatgta cttattgctt cttgccggat tcgtcgtaaa
    30421 cgcttaatcc ggcctaatcc tatgataatt caatatattt cattagaaac cataaacccg
    30481 aaaacatgag taccgggcat tattaaattt ctgaaattac aacaactgtg ccagccggtt
    30541 gatatccgac tgaatcgccc cggcggtgac gtcgcgcccc gcgccaggcc cacggatcac
    30601 cagcgggttg tcgcgatacc agcggctttc gatggcaaag acgttatcgc acggcagcag
    30661 tgatgccagc ggatggtctt cacgcaccgc ctccacgccc acacgcgctt tgccgttggc
    30721 atcgaaacgc gccacgtagc gcagcaccag ccccatttcg cgggccgctt ccagccgttg
    30781 caccatctgc tcgttaagtt catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc
    30841 gcaatgagca ggcaccagcg attccacacg tacctggtcc ggttcaatgt tgtaacccgc
    30901 ttcacgcgcc agaattacca gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg
    30961 atcaggctcg gttaagccct gctgccacgc ctgatccacc agctcggtaa acggcacgct
    31021 accgtcgaat tgcaggaata gccaggagag cgtgccggag aagatcccgc tgatcgacaa
    31081 aatagtatcg ccgctgtcga tcagatcgcg cacggtgtgg ttgatcggca agcccgcacc
    31141 gacggtggca ttgtacagcc agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg
    31201 atatttattg ctgtcgctgg ctcccgccag tttgttggcg ctgataacgt ggaaaccgtg
    31261 gctggcgaaa tcaagatact gatcagcaag ctgctggctg gcggtaacgt ccagcaccac
    31321 taaatcatca tacggatggg cgcgcatcca caggaacaac gactcttcat cctgctcaac
    31381 cgcttcatcg ttgaagaagg ctaacgcgcg gctggcgtcc agcccgtcat agctcaacag
    31441 gctgcggcgg ctgtccacca cacctaccag cacaaactca aagccggtac gtgccgaaag
    31501 cgtgctctgc tcacgggcga acagttccag ccaacgggaa ccgatattgc ccttaccgaa
    31561 caataccagg ccgatgcgtt tttctgcgcg gaagacggac tgatgcagcc cctgaatcag
    31621 gctttcggtc gggccggtgc gcagtactgc caccaggctg atgccgtcat cggactgcca
    31681 ggtaaattcg accggctggc ctttcagttg ctgccagaag cggtggcaat gcagcgggtt
    31741 acgggtgacg cctgcaccga ccatcgccac cagcgccagc ccctgacgca ggcgcagttc
    31801 gccaggtaat cccgcttcgt cgaggatttt cagcgcactg tcggccactt ctgaggtgta
    31861 gcaaaattgc agcaactggc gatcgttatg tacgccaacc gccagcgggc gtacctgcgc
    31921 gcgtttcagg atttggtcga tctctttatg cgccagtttg aaatcctgac tggcgggcac
    31981 ctgaaactca atcaaacaga catcatcgtg gctggtgaca atacgcgcac cagtaccgga
    32041 ggccagcacg cgttcaatgc gcgtggaacc ttgatccggc gtgtagctac agcgcagttg
    32101 caggtcgatt tcgctgccag aaaccggctg taaagtacgg gcgtgaagaa cgggagccgc
    32161 caggcgcgcc agttcgctgg cctcatccag acgcagcaac ggcagcaggc aggcatcttt
    32221 cactttacgc gggtcggcac tgtatacccc ggcgacgtcg ctccagatgg ttacgcgaga
    32281 aacacccgcc agcgcaccga tttgtgtcgc ggaatagtcg gaaccgttac gccccagcag
    32341 caccgtttca ccggcgttgt tgcggctaat aaatccggtc accaccagac gtttgcccgg
    32401 atgttgcacc agcagctgtt gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg
    32461 tgcggcgcgt tcagcgcgta aaaactcgcg ggcatcaagc caggccgctg gcagcccttg
    32521 ttgattaagt accgcagaca tcaggcgtgc cgaccacact tcaccgtgcc ccaccacttc
    32581 cgcatacact gcgtcgttaa taccgctgtc gagcagcgcc gccaggcgct caaggtcgct
    32641 gacaaaagcg ctaatgaggc tatcggcttc ttcagcgggt agcagaccgc taatcagatc
    32701 gcactgatag cgacgcagcg tttgttgaac ctgatgcgca gagagacgat cggtctggct
    32761 tagtttcaac cagttaatca actggttagt ggtgctaccg gcggcggaaa ccaccatcat
    32821 atcgtcaggc tgagagtact ccgccataat gcccgcgaca cgcaaataac acttcacatc
    32881 agccagacta ctgccaccaa atttatgcag ctgacgacct ttcgcccctg cctgcgcaat
    32941 cacactcatt tttacccctt gtttgcagcc cggaagccat tttccaggtc ggcaattaaa
    33001 tcttcgccat cttcaatacc ggtggagata cgcagcagcg tctcggaaat cccggcggca
    33061 gcacgcgctt ctggtgccat gcctgcatgt gtcatggttg cggcgtgaga gattaaactt
    33121 tccactcccc ctaatgattc cgccagcgta aacaacgaca gcccgcccag gaaacgacgc
    33181 agcgtctgct catcgccatc cagttcaaaa ctcaacattg cgccaaagcc tttttgctga
    33241 cgcgcggcaa tttcatgccc ctgattttcc ggcagcgacg ggtgatacag ttttttcacc
    33301 aacggctggg tttgcagata tttcacaatc gcctgcgcgt tacgctgggc caactccatg
    33361 cgcggcacca gcgttcgcaa cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc
    33421 acgccaatat tgtttgccca ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc
    33481 accacgccgg ccaccacgtc tgagtgaccg ttcagatatt tcgtgcatga atgcaacacc
    33541 agatcggcac ctaatgccag cggattttgt aatgccgggc ttaagaaggt gttatccacc
    33601 acgctcaccg ccccgacttc ccttgccaga tggcagattt tcgcaatatc cacgacgcgt
    33661 aacaatggat tacttgggct ttctaccagt accagtttgg gtttttctgc cagcgctgcc
    33721 cgtaatgcct gttcatcgcc ttgatcaaca aacaacacgc gatagcaacc acgtttcgcc
    33781 aggctgtcga acaggcgata gctaccgccg tagcagtcgt gcggcgctac cagcagatcg
    33841 ccaggtttca aaaagacggt cgttaccagg tgaatcgcgg acatgccggt attagtaagt
    33901 actgcaccag caccgccttc cagttctgcc agtgctcgct gaaccacatc gcgcgttggg
    33961 ttgccacgac gcgagtaatc atgcgcgcgc ggttcattaa atccggtaaa gttataggta
    34021 ctggaaagat ggatgggtgg gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta
    34081 cgcactgcga tggtggcctg tttacgcgtc atgtgatgat gttccctggg ctttattggt
    34141 gaaatgtcag gcaccagagt aaacattgtg gtaatggacg tcaatacatc tggacatcta
    34201 aacttctttg cgtatagatt gagcaaatcc caaatagccg ttaaaattat atgcattatc
    34261 acgccaacag gtggattacc cgatgtaacg gtagcgcctg tacggtaaac tatgcgggtt
    34321 tacggccagt gcccacatta actgtgtggt ctggtctcaa tttattgacg aagaggatta
    34381 agtatctcat ggctgaatgg agcggcgaat atatcagccc atacgctgag cacggcaaga
    34441 agagtgaaca agtcaaaaag attacggttt ccattcctct taaggtgtta aaaatcctca
    34501 ccgatgaacg cacgcgtcgt caggtgaaca acctgcgtca cgctaccaac agcgagctgc
    34561 tgtgcgaagc gtttctgcat gcctttaccg ggcaaccttt gccggatgat gccgatctgc
    34621 gtaaagagcg cagcgacgaa atcccggaag cggcaaaaga gatcatgcgt gagatgggga
    34681 ttaacccgga gacgtgggaa tactaaagcg gaaaagagcg gcgcggagtg gaaccgcctg
    34741 atgcgctacg cttatcaggc ctacgtcata ttgcaattta ttgaatttgc acgaacttgt
    34801 aggccggata aggcgttcac gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc
    34861 caccgctttt tatacatgga cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc
    34921 tggtttctgt acctgccttt gcctggcagc cgcaaactgg cgacatcatc tttcaaatct
    34981 ctcgctcatc gcaaagtaaa gcgatccaac tggcaaccca ttccgattac agccacaccg
    35041 gtatgctggt gatgcgtaac aaaaagccct acgtttttga agcggtcggc ccggtgaaat
    35101 acaccccgct caagcagtgg attgcccatg gtgaaaaggg caaatacgtc gttcgccgcg
    35161 ttgaaggcgg actgagtgtt gaacaacagc aaaaactggc gcaaacggca aaacgttatc
    35221 tcggtaaacc gtatgatttc agcttctcat ggagcgacga tcgccagtac tgttcggaag
    35281 tggtgtggaa ggtttaccag aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga
    35341 aagagtttga tctcagcaac ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa
    35401 atattccgct tgaggaaacg gtagtttcac cacaagccgt tttcgacgcg ccacaactca
    35461 ccacggttgc caaagagtgg ccgctgtttt cgtggtaatg gatgatgaaa aaaataattc
    35521 ccgccgtagt ctgtttgttg ctttgtagtt gcacgacctt tccgcaagct gtcgctccgg
    35581 taaacggtga tgcctccaat tagttgaatt aaggatataa tactcgcttt taaggttgtt
    35641 tcatggtcac agttaacctt cattgccccc gttgccagtc cgttcaggtt taccgtcacg
    35701 ggcagaaccc aaaaagacat gatcgattcc gcggccgtga ctgtcaccgg gtatttcagc
    35761 tcagttactg ttacgagccc cgtaaaccgg gagttaaaga ccaaatcgct gaaatgactt
    35821 tcaacggtgc cggggttcgc gatactgccc gtacgctgaa aatcggcatt aatacagtca
    35881 tccgaacttt aaaaaactct cgccacgaag aataacgtca tccccggtcg ctcatgctga
    35941 tgtggcactc atctgcaaac ttagtcagca atggagcttt gtcggcagta aagcgcgaca
    36001 gcactggctc tggtacgtgt acaacacgaa aactggtggt gttctggcct acacttttgg
    36061 cccccggact gacgaaacct gtcgtgaatt gcgggcgctg cttacccttt taccatcggc
    36121 atgttgacca ctgacgaatg gggtagttac gctagagagt taccgaagga gaagcatctg
    36181 actggtaaga tttttactca gcacattgag cgtaacaatc tgaccctgag aacccgaatc
    36241 aaacgtctgg cccgcagaac catctgtttc tcgcgttctg tagaacttca tgaaaaagtc
    36301 atcggtgctt ttatcgaaaa tatatgttct actaattgga gtcatcaccc ttctgataag
    36361 ctttccttgg atgattacca gactgaatac acagccaaag atttttacct cattctgctc
    36421 ttttttctca aatgacatat attatatttt acatcatatt cataggcata tgacattccc
    36481 ccgcccccca ttatgtcatt gtgggttgat gttgggcttt cataaaaaaa aacatctttt
    36541 tttgaactag cattaattat atttacttta tatattaaaa tattttctcc atctggaaat
    36601 attttagcca cacaatataa agcagctgaa tccgaattac ttaaaaatat attatcatca
    36661 cccataactc cagccattaa atgactggag actaaagcaa ggaaataaca acattgtatt
    36721 gccttcattt taattactcc cttgcctgat aattgtaact ccattccctt tagctttggg
    36781 attatatttt agataggtgt tatttggtgt tcccaagtaa ctactgtatt ctttttttcc
    36841 cattccattc atataagaat tcgttaaatc tttgcttgaa aaatttagac tatcataaac
    36901 atcattttct tttgttactt tatttccttt atcgtcagag taaaaaccgt gagtgtgata
    36961 atcgcctact ctctctgaac cttcagggca ttttatattt cgcaagtcta cactatcatt
    37021 atcatttgaa cttatcggat tcatggggaa atattcacct tgcttcttac atattagtcc
    37081 accatattct ttattctcgt taattgattc tccattacac attgccaatg caaatattcc
    37141 aatatcatct atattttttg atttaaattc atataatcct aatggatcca tgctattcac
    37201 cgggctcagc ggatatgtat acaggttcca tccccccttc agcccaatcg gatcctgagt
    37261 gatataccgc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca ggccggactc
    37321 ctcatcatac tgctgccccg gcaggcggat aagctgctgc agctgatgcg ggttctcttc
    37381 attcagcagg ttgccccatt catcgtattc tgcgcaccat tctgttgtcc cttccttgct
    37441 gataag
//
LOCUS       DAFATA010000033        37162 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFATA010000033 DAFATA010000000
VERSION     DAFATA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.033"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(131..544)
                     /gene="entH"
                     /locus_tag="LGJ20_002121"
     CDS             complement(131..544)
                     /gene="entH"
                     /locus_tag="LGJ20_002121"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBK2386176.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(547..1293)
                     /gene="entA"
                     /locus_tag="LGJ20_002122"
     CDS             complement(547..1293)
                     /gene="entA"
                     /locus_tag="LGJ20_002122"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBK2386177.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(1293..2150)
                     /gene="entB"
                     /locus_tag="LGJ20_002123"
     CDS             complement(1293..2150)
                     /gene="entB"
                     /locus_tag="LGJ20_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBK2386178.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(2164..3774)
                     /gene="entE"
                     /locus_tag="LGJ20_002124"
     CDS             complement(2164..3774)
                     /gene="entE"
                     /locus_tag="LGJ20_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBK2386179.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(3784..4959)
                     /gene="entC"
                     /locus_tag="LGJ20_002125"
     CDS             complement(3784..4959)
                     /gene="entC"
                     /locus_tag="LGJ20_002125"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBK2386180.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            5334..6290
                     /gene="fepB"
                     /locus_tag="LGJ20_002126"
     CDS             5334..6290
                     /gene="fepB"
                     /locus_tag="LGJ20_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK2386181.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(6294..7544)
                     /gene="entS"
                     /locus_tag="LGJ20_002127"
     CDS             complement(6294..7544)
                     /gene="entS"
                     /locus_tag="LGJ20_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBK2386182.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            7655..8659
                     /gene="fepD"
                     /locus_tag="LGJ20_002128"
     CDS             7655..8659
                     /gene="fepD"
                     /locus_tag="LGJ20_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBK2386183.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            8656..9648
                     /gene="fepG"
                     /locus_tag="LGJ20_002129"
     CDS             8656..9648
                     /gene="fepG"
                     /locus_tag="LGJ20_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBK2386184.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            9645..10460
                     /gene="fepC"
                     /locus_tag="LGJ20_002130"
     CDS             9645..10460
                     /gene="fepC"
                     /locus_tag="LGJ20_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBK2386185.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(10457..11590)
                     /gene="fepE"
                     /locus_tag="LGJ20_002131"
     CDS             complement(10457..11590)
                     /gene="fepE"
                     /locus_tag="LGJ20_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBK2386186.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(11806..15687)
                     /gene="entF"
                     /locus_tag="LGJ20_002132"
     CDS             complement(11806..15687)
                     /gene="entF"
                     /locus_tag="LGJ20_002132"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBK2386187.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQVLML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(15684..15902)
                     /gene="ybdZ"
                     /locus_tag="LGJ20_002133"
     CDS             complement(15684..15902)
                     /gene="ybdZ"
                     /locus_tag="LGJ20_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBK2386188.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(15905..17107)
                     /gene="fes"
                     /locus_tag="LGJ20_002134"
     CDS             complement(15905..17107)
                     /gene="fes"
                     /locus_tag="LGJ20_002134"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBK2386189.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            17350..19590
                     /gene="fepA"
                     /locus_tag="LGJ20_002135"
     CDS             17350..19590
                     /gene="fepA"
                     /locus_tag="LGJ20_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBK2386190.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            19765..20385
                     /gene="entD"
                     /locus_tag="LGJ20_002136"
     CDS             19765..20385
                     /gene="entD"
                     /locus_tag="LGJ20_002136"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBK2386191.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(20507..20659)
                     /gene="hokE"
                     /locus_tag="LGJ20_002137"
     CDS             complement(20507..20659)
                     /gene="hokE"
                     /locus_tag="LGJ20_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBK2386192.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(21010..21162)
                     /gene="hokE"
                     /locus_tag="LGJ20_002138"
     CDS             complement(21010..21162)
                     /gene="hokE"
                     /locus_tag="LGJ20_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBK2386193.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            21604..22722
                     /locus_tag="LGJ20_002139"
     CDS             21604..22722
                     /locus_tag="LGJ20_002139"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBK2386194.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCENERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            22788..23036
                     /locus_tag="LGJ20_002140"
     CDS             22788..23036
                     /locus_tag="LGJ20_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBK2386195.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLCFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            23101..23469
                     /locus_tag="LGJ20_002141"
     CDS             23101..23469
                     /locus_tag="LGJ20_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK2386196.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            23563..24216
                     /gene="nfsB"
                     /locus_tag="LGJ20_002142"
     CDS             23563..24216
                     /gene="nfsB"
                     /locus_tag="LGJ20_002142"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBK2386197.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            24324..25571
                     /gene="ybdG"
                     /locus_tag="LGJ20_002143"
     CDS             24324..25571
                     /gene="ybdG"
                     /locus_tag="LGJ20_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBK2386198.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(25639..27015)
                     /gene="pheP"
                     /locus_tag="LGJ20_002144"
     CDS             complement(25639..27015)
                     /gene="pheP"
                     /locus_tag="LGJ20_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBK2386199.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(27117..30260)
                     /gene="cusA"
                     /locus_tag="LGJ20_002145"
     CDS             complement(27117..30260)
                     /gene="cusA"
                     /locus_tag="LGJ20_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBK2386200.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVTALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(30272..31495)
                     /gene="cusB"
                     /locus_tag="LGJ20_002146"
     CDS             complement(30272..31495)
                     /gene="cusB"
                     /locus_tag="LGJ20_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBK2386201.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQTEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(31511..31843)
                     /gene="cusF"
                     /locus_tag="LGJ20_002147"
     CDS             complement(31511..31843)
                     /gene="cusF"
                     /locus_tag="LGJ20_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBK2386202.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(31867..33249)
                     /gene="cusC"
                     /locus_tag="LGJ20_002148"
     CDS             complement(31867..33249)
                     /gene="cusC"
                     /locus_tag="LGJ20_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBK2386203.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            33406..34089
                     /gene="cusR"
                     /locus_tag="LGJ20_002149"
     CDS             33406..34089
                     /gene="cusR"
                     /locus_tag="LGJ20_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBK2386204.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            34079..35527
                     /gene="cusS"
                     /locus_tag="LGJ20_002150"
     CDS             34079..35527
                     /gene="cusS"
                     /locus_tag="LGJ20_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBK2386205.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            36264..>37162
                     /gene="tssI"
                     /locus_tag="LGJ20_002151"
     CDS             36264..>37162
                     /gene="tssI"
                     /locus_tag="LGJ20_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBK2386206.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGR"
BASE COUNT         8771 a        10234 c         9527 g         8630 t
ORIGIN      
        1 acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa accaatattt
       61 aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat ctcatttaat
      121 tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa cgccgcccct
      181 gttcatcgaa aacgacgatt tcccagctct gattttgccg cccaagatgc agcggctggc
      241 agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta agttccgtac
      301 ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc agcgtttcag
      361 ccagcgccgc tgacgcgccg ccatgcagca ggccaaacgg ctgatgagta cgggtatcaa
      421 ccggcatttc ggcttccagc acatcatctc ccagacgggt atacacaatt cccagatgcg
      481 ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg cgtttccaga
      541 tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta gggtgatatg
      601 gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg gacgggcgat
      661 tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac gctgttcttc
      721 ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc caggcgaaac
      781 cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg ccaggctttt
      841 cagcgccgct ttcgatgcgc cataagcact catgccaata cgcggcgtgt gcgcggcgtc
      901 ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca tggtttgctg
      961 gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt cctctttact
     1021 gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgaccagca cgtccagtcg
     1081 ctccgtttca gctaacagtc gctgacatac ctgcgcgacc tgcgcagcat cggcaacatc
     1141 catcacttcg gtcgcaaagg gatattgctc ctgagcgaac gcttgatcaa aaccggtaac
     1201 tttcgctccc gcctcaacaa acgccagcgc cgtggcgtag ccgatacctt tacctgcgcc
     1261 ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga gagtagcttc
     1321 caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc accatgcact
     1381 ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta gtcgatcagg
     1441 ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggataac ctcacgcagc
     1501 gccgctttgc tggcggggac aggtgctggc agtaattctt cagtcatcac cacccggcca
     1561 gaccgcccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa atcggccagc
     1621 gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc ggtggtcata
     1681 cagccaatgt gggcatatac cccggtaata atcagctggt tacgtccact ctctttgagc
     1741 atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag caccgtgtcg
     1801 tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgaacg ggtcaggccc
     1861 ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt cggctgggcg
     1921 gtgtaataaa ccggaatatt gtgctgtttg cagtagtcgc gcagcgcagc aatattcgcg
     1981 atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata gtcctgcata
     2041 tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt attcttaggg
     2101 atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat cgtgttctct
     2161 ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat cgactttccc
     2221 gaccgccgtc agcggaagtg aatctacaca ctccacgcga tccggtaatt taaattcggc
     2281 aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct cttttaccac
     2341 cagataagcg cagctttttt cgcccatcag ctcatcttcc atgctcacca gtgcggcgta
     2401 gatcaccgcc gggtggcgca gcagcaggtt ttcgatctct tcggcagcga tcttctcacc
     2461 gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtacccct ctggatcaat
     2521 agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg cattgtgctg
     2581 cggacttttg taataaccgc ggaaggtgta cggcccgcgc gtcatcaggc gtccaacttc
     2641 cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt ccggacacat
     2701 tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg tgtagttcac
     2761 cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag cgggaatacg
     2821 cgccgcaagg gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca gcgaggcaag
     2881 ctgcgcccgg ctttcacctt cggtcagcgc ctgcaaccac aggctgactg ccggtggcac
     2941 cagcgcggtg acgttcacct gatgtttttc aatcaatggg aagcaaagcg tggcgctggg
     3001 atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc ccggcgaact
     3061 catggcgtag ttatgagccg ccgggatcgc gcacaggtag cgtgtctgtt gtgtgaactg
     3121 acaaatctcg acgctacgac gcacgctgta gtagtagtcg ttatgagtgc gcgggatcag
     3181 tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat cagcaggtga
     3241 tggcgtggca gtaaaatcct cagccggatg gttaatcgca tcctgcaagt tatgctcacc
     3301 gctgtcgttg tgcagttgca ccacgcgaat ggaggaatgt tctgtgacga aagtattgag
     3361 gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca atcagcaatg cgggttcaat
     3421 ctggctggca taggcgttca gttcactacg ctgatggctg aacagcgcca gcaccggcgc
     3481 aacgcccagt ttcagcagcg cgaaaaaggt aatatacaat tcagcgacgt tacccagttg
     3541 taccagcgcg gtttcaccag gtttaatgcc ctgacggcgt aaactacacg cgaggttatc
     3601 cgccgcctga ttcagctccc ggtaactcaa ctgtcgctcg ccgtcgataa ccgcgatgct
     3661 gtcactcgca gcgtggcgag tcagaatgtc ggtcagcggc aaatcctgcc agtagccttt
     3721 ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc tcatcctcgc
     3781 tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg tttcgcgcca
     3841 ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac gcacctgatt
     3901 ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt cgctgtcaca
     3961 ccaacccaca atgccgccaa acagttcgcg gtcgaatggc tccagttcag caataacctg
     4021 ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtt ggatgcagca gacaggccag
     4081 agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga gatgccacag
     4141 cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac tgcgttcgcg
     4201 cagtacctct ttcatcgcct gagtcaccag ttcatgttca tggcgatctt tttctgacgc
     4261 cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac gcgcggaacc
     4321 ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt ccgggctggc
     4381 ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa ccgggttttg
     4441 cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg tgatatcaat
     4501 caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg cggcgcgggc
     4561 aaccatctgt tcaaacgtgg tttgttcagg aattgcctgg cgttccacca cgttcagcga
     4621 ctggctgcgg gtaaaacggc gggctgaggt ctgtttttcc tggcgggaga acgactgcca
     4681 ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg ccccgaccat
     4741 caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga gtttttgctg
     4801 gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaagcggg cgaaacatcc
     4861 tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg caagtgttgc
     4921 catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca caaaatgata
     4981 aaggctttaa taatgattat catttatatt ttcggtcgct aacctaaaag caaacgcgca
     5041 ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg ttgtggttac
     5101 aatgaaaatg agaagcatta ttgatggatt cgcataagcg caatgtgatg tcctgcgccg
     5161 ttctgccccc tctcccttcc agggtgaggg ctggggtgag ggttaatgtt cgcaccagtg
     5221 ctggctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggacgg attgtgcgct
     5281 ttgtcgaatt tgtcattacg cccttaacct tattaataac aggaagctga tttgtgagac
     5341 tcgccccgct ctaccgcaac gcccttctat taacaggact tttactttca ggaatagccg
     5401 cagttcaggc cgctgactgg ccgcgtcaga ttactgacag ccgtggcaca catacactgg
     5461 aaagccagcc gcagcgtatt gtttccacca gcgtcaccct gaccggctca ctgctggcga
     5521 ttgatgctcc ggtgatcgcc agcggcgcga ccacgccgaa taaccgcgtc gcggatgacc
     5581 agggcttttt acgccagtgg agcaaggtgg cgaaagaacg caaactgcaa cggctctata
     5641 tcggcgaacc gagcgccgaa gccgttgccg cgcaaatgcc ggatctgatt ttaattagcg
     5701 caaccggcgg ggattcggcg ctggcactgt atgatcagct ttccaccatc gccccgacat
     5761 taatcatcaa ttacgacgac aaaagctggc agtcgctgtt aacgcagctt ggcgaaatta
     5821 ccgggcatga gaaacaagcg gcagagcgga ttgcacagtt tgataagcaa ctggcagcgg
     5881 cgaaagagca aatcaaatta ccgccgcagc cggtcactgc cattgtctat accgccgctg
     5941 cacacagtgc caatctctgg acgccggaat cagcacaagg gcagatgctg gaacaactcg
     6001 gctttacgct ggcgaagttg cccgcagggt taaacgccag ccaaagccag ggcaaacgcc
     6061 atgacatcat tcagcttggt ggggaaaatc tggctgctgg gttaaatggc gagtcactat
     6121 tcctgttcgc cggtgatcag aaagatgccg atgcgattta tgccaatcca ctgctcgcgc
     6181 accttcccgc agtacaaaac aagcaggttt atgcgctggg aaccgagacg ttccgtctgg
     6241 attactacag cgccacgcaa gtgctggaac gacttaaggc gctgttttaa gcattaactg
     6301 tcagacgctg tcacctgcgg cggcgtctgg cgaaaacgtc gcaactccac cagcaccagc
     6361 aataacaaca cgccgataat caacaaacca aaaccgcttg cgcttgcgga agcaaccggt
     6421 gtcatcatcg cgcccaaacc acccagcagc gccgcgccta tggcatcgcc cgtcacgttc
     6481 tgcgccgtcc acaaaccgtt aatccgccct aacatcgctt ccggggtttg cgtttgcagc
     6541 attgtatatt gcagcaacga actaaccgcg ctcagccagc cgaacagcgc cagacaaacc
     6601 acgcctaaaa tccacatcgg catcaggcca aacagaccaa tggcgaggaa cgatcccagc
     6661 gtggagagca gcatcaataa ccctggtcgc gcactatgtg ccagcttccc gctggttaac
     6721 gcaccaatag ccgcgccgag cgggatcgcc gcgtagagaa aaccaatctg tgccgctgac
     6781 atctgccagt tgtcagccag cgccggatac agtacccgca ccgcgctcgc catcgtcaat
     6841 aaaccaccca gcagcgcaat cccgcccacc agcgggctgg caagcagaaa acgaaatcct
     6901 gccagtaatg atttcaacgg atgctcacgc ggctgcggtg gcggtggcaa cgccggaagg
     6961 cttaacaacg gtagcaaggt aataaacgtg cccgccgccg ccagcccgta gttccaggct
     7021 acgccaccgg tcgctagcaa taaaccgcca atcatgggcg aaatcaccga ccccagacgc
     7081 acggtcaaca tggtgatcgc cccggcctgc attaagtttt cacgccctac cagtgctggt
     7141 gtcgccgcca atagcgccgt aacgccaagc gatgcgaaaa aaccatccca taaaccaagt
     7201 aaatagattg ccagcaatga cggctccggc agcagtgcat taaggcacag tccaatgaag
     7261 ccaatgccac aggtgccgcg cgccagcaaa atcacttttt tgcgctcata gcgatccgcc
     7321 agcacaccgc caaccatcag gccaacaaac atcgcgccgc cggtcagcgt caccgaaagc
     7381 cccacctgcc aggtagaatg cgtcatcatc tggatctgca ccggcaccgc gacgccgagc
     7441 aaacccagag acacaattga gataaaacga gcgaggaata ctgcgcgaaa cgccgggtgc
     7501 gttttcaaca ggctgaggtt aagcagccag gattgtttat tcattacaat gccttgccat
     7561 caaatatttt aaatacctat tcttaggctg cacatgctaa catatccaaa taagatcgat
     7621 aacgataatt aatttcatta tcatggaagt tcgtatgtct ggttctgttg ccgtgacacg
     7681 cgccattgcc gtgcccggat tgctgttatt actgattatc gcgacggcat taagcctgct
     7741 cattggggca aaatcactcc ccgcttccgt agtgctggag gccctctccg gcacctgcca
     7801 gagcgccgac tgcaccatcg tgctcgacgc gcgactgccg cgcacccttg ccggtttact
     7861 tgcaggcggc gcgcttggcc ttgccggggc gttgatgcaa accctcaccc gaaatccact
     7921 tgccgacccc ggcttgcttg gcgtgaatgc cggagccagc tttgccattg tgctgggtgc
     7981 ggcgctgttt ggttactcat ccgcgcagga acaactggcg atggccttcg ccggggcgct
     8041 ggtggcctca ttgattgttg cctttaccgg cagtcagggc ggcgggcagt taagtccggt
     8101 gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg gaaggactga ccagcggcat
     8161 cgccctgctt aatcctgacg tctacgatca actgcgtttc tggcaagccg gttcgctgga
     8221 tattcgcaat ctacatacct taaaagtggt gctgatcccg gttctgatcg ccggagcaac
     8281 tgcgctatta ctgagtcgcg cgctgaacag tttgagcctc ggcagtgaca ccgcgacggc
     8341 gctgggcagt cgcgtggcgc gcacacagtt aattggtctg ctggcgatta ccgtgctttg
     8401 tggtagtgcg acagcggtag ttggcccgat tgcctttatt ggcctgatga tgccgcatat
     8461 ggcgcgttgg ctggtgggtg ccgatcatcg ctggtcgctg cccgtcacgc tactcgccac
     8521 ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg attgtgcccg gcgagttgcg
     8581 cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg atcttcctcg tgcgacgtaa
     8641 aacgcgaggt ggtgcatgat ttacgtctct cgccgattac tcatcacctg tttgctgctt
     8701 atttccgcct gtgtggttgc aggtatctgg ggattacgca gcggtgccgt cacgctggaa
     8761 acctcgcagg tattcgccgc gctgatgggc gatgcgccgc gcagtatgac gatggtggtc
     8821 accgaatggc gtttaccacg cgtgctgatg gcgctgttga ttggcgcggc actgggcgtc
     8881 agcggtgcga tttttcagtc gctgatgcgt aacccgctcg gcagccctga cgtaatgggc
     8941 tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg tgctgtttgg tcaggacctg
     9001 acggctatcg cgctgtcagc aatggtgggc ggcattgtca cttcgcttct ggtctggctg
     9061 ctcgcctggc gtaacggcat cgacaccttt cggttgatta ttatcggtat cggtgttcgc
     9121 gccatgctgg tggcctttaa tacctggctg ttgctgaaag cgtctttaga aacggcgcta
     9181 acagcgggtt tgtggaatgc cggatcgctc aacggcctga cgtgggcaaa aacctcgcct
     9241 tccgcaccca tcattatatt gatgctcatt gccgccacct tgctggtgcg gcgaatgcgc
     9301 ttgctggaga tgggcgatga taccgcctgc gcgctgggcg tcagcgtcga acgttcgcgt
     9361 ctgttaatga tgctggttgc agtggtgctt accgctgcgg caacggcgct tgccgggccg
     9421 atttccttta ttgctttagt cgcgccgcac attgcccgac gcattagcgg caccgctcgc
     9481 tgggggctga cccaggcggc gctgtgcgga gcgctgttac tgctggcggc cgatctctgc
     9541 gcccaacaac tgtttatgcc gtatcaactt ccggttggcg tcgttaccgt cagcctcggc
     9601 ggtatttacc ttatcgtctt gttaattcag gagtctcgca aaaaatgacc gaatcagtag
     9661 cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa atataccgtt gcggaaaatc
     9721 tgactgtaga aattcctgat ggtcacttca cggcaattat cggaccaaat ggctgtggta
     9781 aatccacgtt actgcgtacc ttaagccgcc tgatgacgcc tgctcacggg catgtctggc
     9841 tggatggcga gcacattcaa cattacgcca gtaaagaagt tgcacgccgg attggtctgt
     9901 tggcgcaaaa tgctaccacg ccgggcgata tcaccgtgca ggagctggtg gcgcgtggac
     9961 gttatccgca tcaaccgctg tttacccgct ggcgcaaaga ggatgaagaa gcggtaacga
    10021 aagcgatgca ggccacggga ataactcatc tggcagatca aagcgtggat accctttctg
    10081 gcggacaacg ccagcgcgcg tggatcgcga tggtgctggc ccaggaaacg gcaattatgc
    10141 tgctcgacga acccacgacc tggctggata tcagtcatca gattgatcta ctggaactgt
    10201 taagcgaact gaaccgcgag aaaggctata ccctggcggc ggtgctgcac gatcttaatc
    10261 aggcctgtcg ttacgccagc catttgattg cattgcggga agggaaaatt gttgctcagg
    10321 gagcgccgaa ggagattgtc actgctgaac tgattgagcg catttatggt ctgcgatgca
    10381 tgatcattga cgatccggta gccggaacgc cgcttgtggt gccgctcgga cgaacggcac
    10441 cttcaaccgc aaatagttaa actaagtggt ctgccatcat ggcatcctgt tttctggatg
    10501 ccatcgcata gcgcaataac acgctaccac aagccaccat cccgccgatc agcgcggaaa
    10561 ggatcacaat aatcgcctta cccggaccgt cttttttcac tggcaatgac gggcttaact
    10621 gatatttaaa cggcgtaaaa ttcacatcgt tgatatttgc ttttgttaac tgctcaacaa
    10681 gatactgacg attacgtaat tcaccgttca gttccgcaac gtcagtgacc gctttttcaa
    10741 tttccagttt gcgttcaata ccgtctgcgc cgagagaaat agagaaatcg gggtcatctt
    10801 taacggcctg accattactg tatacgggct ttttaattcc cgccgcgttg gcaatgtcga
    10861 gtgaataatt gaggcgctga atgtttgcat caagttgatt tttcgtttta atgcgatcct
    10921 gagccagttt ttctttttca aactgggttt tgatctccag tttattacgg acgttttcta
    10981 tcgactcttt caccaccaac gcagagatat aatcgatata cccgctcaaa acggtctgcg
    11041 cctctttact ggttggcgcg gtaaaactta gcgtccagga ggtatacagt gacggttcat
    11101 cttttttctt actggcattg tcatcaaccg ctttcatttt ttcgctcaat gcaacaatag
    11161 cgcgatgcaa atccagttca tcgattttcg cctcttttaa ttggtccatc acataaggtg
    11221 atgaacgcag gtactcttcc agcaagctaa ccgactgaaa cttcttgata aacaggttaa
    11281 atgcttctgt acgatcaatt ttgatatcca gatccagcac acgaagctta gtgaatgttt
    11341 tctccaactc ttgccactga acaggttctg gaggcgtgac aaccgccgcg ctggtccatt
    11401 tttgcggcag gatgaaagag atcagcaagc ctgcgcaggc aaacgcaaaa acgaccgcca
    11461 tgaccgtttt tttggcccgc cataaaactg agattagatt aagtaaatca atttcattat
    11521 tactgggcga cgccagagga taatcgggaa aatgagcgtc acttccctgt ttaatattca
    11581 gtgatgacat ggggtataac ctgaaaatga gtccaatagg gggaaatctc cacggcgctc
    11641 actttaccag ttggtgagaa ttatccgaag ctgaagtttg taaattcctt ccaatgaatg
    11701 caacattcac tctatttaag agaaataacc agcaaactta taaattaaac aaatgattaa
    11761 cccataatta tttcctgtaa ttatgggtta atagataatg ttaatttacc tgtttagcgt
    11821 tgcgcgaata atcggcccaa ttttttcaaa cgcccctgga gagataatat ccacatgcgc
    11881 acaatcctga cgatagatat ccagctccgc tatccacggc gaccaggcgc gttcgggact
    11941 cataccttcc tgaagtgtgc gttcagcaac aaacagcgtc gctttaccgt caaacggtac
    12001 gctatgagca gtcgtcagca ggcgcacagc atcagcgtag ttgccttcaa tggtggtaaa
    12061 caactccgtt gaagtacttc cctgctgtgc tgccaggaag gcctcgcgtt cgcggttaat
    12121 ctccgccagc acttccgggt ccagaccatt agcttctttt tcctgccagt tttgcgtttc
    12181 tggcggccag gtatccagca agccaagaaa tgccacctgt tcgccacggg cacgcagtcg
    12241 cgccgcaata ccctgcgcca gcgtaccgcc aagggaatac cccagcaggt aataagggcc
    12301 gtgcggttgt tgctcaagta acgttgccag atgcgcttcg cagacttcat ccaggtttgt
    12361 cgctgtctgc atgggtccat gagggcgcgg cgactgaatg ccaataatcg accattgtgg
    12421 atcgagataa cgcgagagca cgctaaactg ccaggcaaaa ccggacgccg gatggaaaca
    12481 aaacagcgtc gggccattac cttcacgcaa cggcagaatg gtttcgaatc ccatgcgccg
    12541 ggagctgtcc tcttcaccat caataatcgt tgccagtttg gcgacggttg acgcgaccat
    12601 cacctgcccc ggcgtcacct gacgagcaaa ctgtcgactt aactgcgctg ctagtttcat
    12661 tgccagtagc gaatgaccgc ccagcgcaaa gaaatcagca tcggcatcct gcacgtcaca
    12721 acccagcaac gacgcgaatg ccgcggcgat aatcgtttca ctgcccgctt tcggtgcacg
    12781 tcccgacgct tgtgtcttca gttcaggcaa cggtaaggct ttgcgatcca gcttgccgtt
    12841 ggcgctaagc ggcaactgcg gaagttgcag cagaacgacc ggcaccatat gcggcggcaa
    12901 tgtttcgcga agctgtgcct gtaatgcgct ggtatccaac ggcagtcccg attgcgacac
    12961 cagatagccc accaactgac gcgcatcacc accggtggct gccgcctggt taatcacaca
    13021 ggcgtgggta actgcctgtt cgacatccgg cagcgcctgc atcacgcgat cgatttcgcc
    13081 cagttcgata cgctgcccgc gaatttttag ctgatcgtca ctgcgcccga ggtactccac
    13141 cgcgccgtta tccagccagc gggcgacgtc tccggtacga tacatccgtt cacctggggc
    13201 aaaaggatcg gcaataaaac ggctggcggt cagatcgggt cgtccaagat acccctgcgc
    13261 cagttggatg ccggtgagat agagatcacc cgccacaccc ggcggcaccg gatgcatcat
    13321 cgcatcaaga atacgcagac ccgtattcca taccggataa ccaatcggca cactgctgcc
    13381 gcgcacctgt gccagttcct cgccaaaagc cggataccag ctgacatcta ccgccgcttc
    13441 cgtcgggcca tatagattat gcaacggcgc gccagttaac tgttgccatt cgcggcataa
    13501 atcagccggt aaggcctcac cactacagaa aacctgtttc aacgtcgcgc aactctggcg
    13561 agcggtttgc ggcgtcagcg aggcaacaaa tgccgccagc atcgacggca caaagtgcgt
    13621 ggtcgttacg ccatattcgg caaagaattg ctgcatagcg agcgggtcgc ggtgcgcttc
    13681 cggttcagcc atcaccagtt tcgccccggc aataaacggc cagaaaaact cccacaccga
    13741 gacatcaaaa ctgcacggcg ttttttgggc aacgacatct tcgcctgtaa gcgggtaatg
    13801 gttttgcatc caaagcaagc ggttaacgat agccgtctgc ccgaccatta cccctttcgg
    13861 cctgccggtg gagccagagg taaagatgat ataagccgtg tggtgcggtt gtgaaagttg
    13921 cagcggcgca ctgccctggg gggtaagcgg ggcgttatag caaaggcttg ttaaattggg
    13981 aacatcgcta aagcgcggca gttgatcgtc ggtggtaatt aacagcgacg gacgcgcatc
    14041 ttccagcatc attttcaggc gatcgtccgg atagccggta tccagcggta gccaggccgc
    14101 gccagcttca actattgcat ggagtgccag ggtcaaaaag accgagcgcg gtagtgccac
    14161 cgccacgctg tcccctggtt taacgccgcg ctcacgcagc agattcgcca gcgccaccac
    14221 ctgctcgcgc atttcccgat agctgaacag gtaacgcgca tctgccagcg ccggagcatc
    14281 cggtgttttt gccgcttgtt ctgccaccag cgcgctcagc gtggtttctg gaatctcaac
    14341 ctgagtggcg ttgatctgcg ccagctgcgc atactcacct ggcagcataa tatcgacatc
    14401 gccgcacaac agcgccggat ccgcggcgaa ctgggcaatc agcattttca ggcgttcagc
    14461 atgctggatt aacgttggct catcgtaacg ctgtttattg gcgaggatct caatactcaa
    14521 atcaccgtgt tcatccggga acagggccag ttcaaggtca ttaaccggac cggttgccag
    14581 ggtatgggtt tgcgtctgaa caccaggaat atccagttgg taatcaaata ccttgatatt
    14641 gagtaccgga ccgaacagcg gttcctctcc tgccgctcgc ccgctgtcac ggacaatttg
    14701 ttcggcatcg taacgttgat gacgacgcat ttttttcagt tgtgctgcca gtcgggttgc
    14761 cagttccggc agcgtttctt gcgccgcaat gtgaataccc aacggtaaaa cgttgagcac
    14821 gggtccgcta gccgtcagcg ccgccgagcc cagtcgacgc ataaagataa atccggcggc
    14881 atagtccatg cgattgcaca atcgccccag ccacaaggct gccagcgcaa gggctaaatc
    14941 ggtacgctgc acacctgaaa gttgcgtagc cagctggcgg aattccccgt cggtaaattc
    15001 cagtttcagg cgcagaatat ctgccgaagc gctgcgcccc gctaaaggtg ccggagaaag
    15061 tgacgcgggc ggcggcagtt gacgacgctg ttctgcccag aatgccgcat cacgctgcca
    15121 ggcttcgctt tcgcggtatt gctggtactc ttccactaca tcagcgaaag gcgtaaacgg
    15181 cgaagcaggc gttggttcgc cacgcagcca tgtgcagtaa atattggcga tctggcgagt
    15241 aatggccggg aaactgaagc catcgaccag caaatggtga taacgctgat accagtacca
    15301 gcggttatcc gcaacatgta tcagctgatg aaagaccagt ggtttaccgc tatcgacgcg
    15361 caaatcttgt tgcaaatccg ccagcattaa tacctgcgca gtaccgtgcg gatcaatatt
    15421 ggttcgcagg tcgataattt ctggcagttc gaatatcagc gcatcatcga cccactgcca
    15481 gacttcgccg ttatcttccg taaaacgcat ccgcaacgta tctgcttgcg ctagtccgac
    15541 aaccaccgcg cgggccagta atggcgcatc aacatctcca gttaactcaa cgtaatgcgc
    15601 cacgctccag gcggagggta aatccgacag tttttctgcc atccagatgc cgggctgtgc
    15661 ggcgaccaaa ggtaaatgct ggctcattgt gcctcctgca actgggtgaa attggccggt
    15721 gtcagagtac gccagtggac ttccagccac tgctggcagg acgcctgtga ctgcggctga
    15781 cacacaatgt cccagcctgc cggtaaggcg cattgttgcg gccacagact gaattgcccc
    15841 tgcgcattgc gcaaaatgta aaacgctccc tgtggatcat cgaagggatt actgaatgcc
    15901 atattcaact cctgtcatgg aaaagtggtt gccagaggtc gattagcccc tgcatcaagc
    15961 caccgcgcca acaaagcgca tcatgtccgc cgtcaacctg acgccagaaa atggattctt
    16021 ttatggggtg taattgcgca tacagcgcct gattggcccg cataatcatc ggctcgcgaa
    16081 tacccgcttc cagcacaatg cgcagacctt cggcgctaac ttcaccagct ttaagctttt
    16141 caagtaacac gccctcttgc tgcccgcccc gatgcggcca ccagtacgat cctgactggc
    16201 ttaatacaca gccaaagcgt tcaggccagt gcagtccggc atacagcgcg gacagcccac
    16261 caaaactctg cccggcgacc acggtgcgat cggcgcgatc gctaaaaggg gcaatagctt
    16321 tcaccagggg taataactct tgctgtactg ccagccagaa atccgcatta cacggcagtt
    16381 cgtgggcgcg gtgcgtggtg tcgatagcgt cgatcaacac atacacggcg ggaggaagtt
    16441 gctgacgatg ggtcagcgaa gtcagcacag gccagacggg catactttgc gcccaaaatt
    16501 cgccatcgag caaaactgcc agcggacgtt cttctgctgt tgcatcgccg gtggtaaaaa
    16561 tccatacacg acgtgaattt ttcaaccgtt cacttttcca gataatttct ttggctgcta
    16621 tttctggcgc ttgcggacaa tcccatcccg gttgcagagg cgcttgcggc atttcgagtg
    16681 cagaaacagc gtgccctcgc ccgcctttcc agctttgtgg attcagcgga tcggctattg
    16741 cctggggtaa tagttttcgc cagccttcac gtaattcgag gcgatcgggg gatggtgcag
    16801 aaaaaatgtc atcgcgttcg gtgggaataa agcagtagct gccgcgccag ttggcattga
    16861 gttgtgtcgt ccactgccag acatcagtac ctgcaattcg ctgcatcgac tggggctggc
    16921 tgttctgatg atgatcggtc acaccagtga tgtagatcca tacgcgcttt atcgtcgagt
    16981 attcttcaga accttgggga tcacgccacc agaaagtgac ctcaaacatt tcgtcattca
    17041 gacgctgcca ttccgggcca tgtttcgact gccaccagct ctcacttcct acttttaacg
    17101 ccgtcacacc ataaccccat gtttactgtg caatttttca ttgattgcag aaatatattg
    17161 ataatattat tgataactat ttgcatttgc aatagcgtaa tggcgcgccg tgggaagcgc
    17221 ggacatttat taaccaactg cactgcgtgt ctttcaggat caaaggtttt cgcggtagcg
    17281 ggatgcgtcg tgttgaggac gaccatgccc gacagttgca attcgtggca aaaatgcagg
    17341 aataaaacaa tgaacaagaa gattcattcc ctggccttgt tggtcaatct ggggatttat
    17401 ggggtagcgc aggcacaaga gccgaccgat actcctgttt cacatgacga taccattgtc
    17461 gttaccgccg ccgagcagaa cttacaggcg cctggcgttt cgaccatcac cgcagatgaa
    17521 atccgcaaaa accccgttgc ccgcgatgtg tcggagatca tccgtaccat gccaggcgtt
    17581 aacctgaccg gtaactccac cagtggtcag cgtgggaata accgacagat tgatattcgc
    17641 ggtatgggtc cggaaaacac gctgattttg attgacggca agccggtaag cagccgtaac
    17701 tcggtgcgtc agggctggcg tggcgagcgc gatacccgtg gtgatacctc ctgggtgcca
    17761 cctgaaatga ttgaacgtat tgaagttctg cgtggtccgg cagctgcgcg ttatggcaac
    17821 ggcgcggcgg gcggcgtggt taacatcatt accaaaaaag gcagcggtga gtggcacggc
    17881 tcctgggacg cttatttcaa tgcaccagaa cataaagagg aaggtgccac taaacgcacc
    17941 aacttcagcc tgaccggtcc gctgggcgac gaattcagct tccgcttgta tggcaacctc
    18001 gacaaaaccc aggctgacgc gtgggatatc aaccagggcc atcagtccgc gcgtgccgga
    18061 acgtatgcca cgacgttacc agccgggcgc gaaggggtga tcaataaaga tattaacggc
    18121 gtggtgcgct gggacttcgc accattgcaa tcgctggaac tggaagcggg ttacagccgc
    18181 cagggtaacc tgtatgcggg cgatacccag aacaccaact ctgacgctta cactcgctcg
    18241 aaatatggcg atgaaaccaa ccgcctgtat cgccagaact acgcgctgac ctggaacggt
    18301 ggctgggata acggcgtgac caccagcaac tgggtgcagt atgaacacac ccgtaactcg
    18361 cgtattccgg aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa agcgacacag
    18421 gatttcgtcg atatcgatct cgatgacgtg atgctgcaca gcgaagttaa cctgccgatt
    18481 gatttcctcg ttaaccagac gctgacgctg ggtacggagt ggaatcagca acggatgaag
    18541 gacttgagtt ccaataccca ggcgctgacc ggggcgaaca ccggcggcgc tattgatggt
    18601 gtgagtgcca ccgaccgtag cccgtattca aaagcagaaa ttttctcgct gtttgccgaa
    18661 aacaacatgg agctgactga cagcaccatc gtaacgccgg ggctgcgttt cgatcatcac
    18721 agtattgtcg gcaataactg gagcccggcg ctgaacatat cgcaaggttt aggcgatgac
    18781 ttcacgctca aaatgggcat tgcccgtgct tataaagcac cgagcctgta ccagactaac
    18841 ccgaactaca ttctctacag taaaggtcag ggctgctatg ccagcgcggg cggctgctat
    18901 ctgcaaggta acgatgacct gaaagcagaa accagcatca acaaagagat tggtctggag
    18961 ttcaaacgcg acggctggct ggcgggcgtc acctggttcc gtaacgatta tcgcaataag
    19021 attgaagcgg gctatgtggc tgtagggcaa aacgcagtcg gcaccgatct ctatcagtgg
    19081 gataacgtgc cgaaagcagt ggttgaaggt ctggaaggat cgttaaacgt accagttagc
    19141 gaaacggtga tgtggaccaa taacatcact tatatgctga agagtgaaaa caaaaccacg
    19201 ggcgaccgtt tgtcgatcat cccggagtac acgttgaact caacgctgag ctggcaggcg
    19261 cgggaagatt tgtcgatgca aacgaccttc acctggtacg gtaagcagca gccgaaaaaa
    19321 tacaactaca aaggtcagcc agcggttggc ccggaaacca aagaaatcag tccttacagc
    19381 attgtgggtc tgagcgcgac ctgggatgtg acgaagaatg tcagtctgac cggcggcgtg
    19441 gacaacctgt tcgacaaacg tttgtggcgt gcgggtaatg cccagaccac gggcgatctg
    19501 gcaggggcca actatatcgc gggtgccggg gcgtatacct ataacgagcc gggacgtacg
    19561 tggtacatga gcgtaaacac tcacttctga tgctaacgtc agattgttga caaagtgcgc
    19621 gtcgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaatc ttgccaattc
    19681 aatatattgc aggaccatgt aggcctgata agcgtagcgc atcaggcagt ttagcgtttg
    19741 tcatcagtct ctaatatggt cgacatgaaa actacgcata cctccctccc ctttgccgga
    19801 catacgctgc attttgttga gttcgatccg gcgaattttt gtgagcagga tttactctgg
    19861 ctgccgcatt acgcgcaact gcaacacgct ggacggaagc gcaaagccga acatttagcc
    19921 gggcggatcg ctgcaattta tgcgctgcgg gaatatggct ataaatgtgt gcccgcaatc
    19981 ggcgagctac gccaaccggt gtggcctgcg gaggtatacg gcagtattag ccactgcggg
    20041 gctacggcat tggccgtggt ttcccgtcaa ccgattggcg ttgatatcga agaaattttt
    20101 tctgcgcaaa cagcgaccga attgacagac aacattatta caccagcaga acacgagcga
    20161 ctcgcagact gcggtttagc cttttctctg gcgctgacac tggcattttc cgccaaagag
    20221 agcgcattta aggcaagtga gatccaaact gatgcaggtt ttctggacta tcagataatt
    20281 agctggaata aacagcaggt catcattcat cgtgagaatg agatgtttgc tgtgcactgg
    20341 cagataaaag aaaaaatagt cataactctg tgccaacacg attaattgac aacatctggt
    20401 acgattcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt gccttaccac
    20461 tctgacctga caaccggata tgcggggatt gctccccgca caacggctac ttcttcggtt
    20521 cgtaagcgag aacagcctta aactcaatat tacgttcctt tacggtgaac tcacacagcg
    20581 agtctccgac cagaagcgta aatcccagta ccgtcaaaca cagcactatg actgccacaa
    20641 gggcatattt cgtcagcatg tttatattgc ctccatgcga agaagggact accatccgag
    20701 ttgttgaggc tcggagtggc agccccagga ttgatagaaa tatctcctgg ggcttcatct
    20761 ttccagacct cagagttaac ctgaaaccgg aaagcttcag gcacccgccg ttatcttacc
    20821 cttcctcaac aaaatagctg tttatttata cagtgaatcc tgtttcggac ttctgtcggc
    20881 aaaactcatc attgctctta aaacgaaatc gcccgcagcc atcactgacc gcgggcgaaa
    20941 gtgtaaagca ggtgccttac catcctgacc tgacaaccgg atatgcgggg attgctcccc
    21001 gcacaacggc tacttcttcg gttcgtaagc gagaacaacc ttaaactcaa tattacgttc
    21061 ctttacggtg aactcacaca gcgaatcccc gaccagaagc gtaaatccca gcaccgttaa
    21121 acacagcact atgactgccg caagggcata tttcgtcagc atgtttatat tgcctccatg
    21181 cgaagaaggg actaccatcc aaattgttca ggtttgggta ggtagcccca gggttgatag
    21241 aaatatcgtc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
    21301 tcaggcaccc gcagttatct tacccttgct tagcaaaata gctgtttatt tatacagtaa
    21361 gtcctgtttc ggacttctca ctccccattt gatcaactga agctgaaccc gtttttttct
    21421 gcatgcctca atcgtcaaaa ttcttcgcca ccagccagcc gtgccataac ttgtcacatt
    21481 gcaaaagtag ccaaattgct tcagtcacca tcaccaaaaa aagaaacatt ccagaacttc
    21541 cgcatctcgc ccagccgctc cgacttctat actgaataga aaacgccaac ttaagagaaa
    21601 cctatgccat tacccgattt tcatgtttct gaacctttta ccctcggtat tgaactggaa
    21661 atgcaggtgg ttaatccgcc gggctatgac ttaagccagg actcttcaat gctgattgac
    21721 gcggttaaaa atgagatcac ggccggagag gtaaagcacg atatcaccga aagtatgctg
    21781 gagctggcga cggatgtttg ccgtgatatc aaccaggctg ccgggcagtt ttcagcgatg
    21841 cagaaagtcg tattgcaggc agccgcagac catcatctgg aaatttgcgg cggtggcacg
    21901 cacccgtttc agaaatggca gcgtcaggag gtatgcgaaa acgaacgcta tcaacgaacg
    21961 ctggaaaact ttggctatct catccagcag gcgaccgttt ttggtcagca tgtccatgtt
    22021 ggctgcgcca gtggcgatga cgccatttat ttgctgcacg gcctgtcacg gtttgtgccg
    22081 cacttcatcg ccctttccgc cgcgtcgccg tatatgcagg gaacggatac gcgttttgcc
    22141 tcctcacgac cgaatatttt ttccgccttt cccgataatg gcccgatgcc gtgggtcagt
    22201 aactggcaac aatttgaagc cctgtttcgc tgcctgagtt acaccacgat gatcgacagc
    22261 attaaagatc tgcactggga tattcgcccc agtcctcatt ttggcacggt ggaggttcgg
    22321 gtgatggata ccccgttaac ccttagccac gcggtaaata tggcgggatt aattcaggcc
    22381 accgcccact ggttactgac ggaacgcccg tttaaacatc aggagaaaga ttacctgctg
    22441 tataaattca accgtttcca ggcctgccgc tatgggctgg aaggcgtcat taccgatccg
    22501 cacactggcg atcgtcgacc gctaacggaa gataccttgc gattgctgga aaaaatcgcc
    22561 ccttccgcac ataaaatggg tgcatcgagc gcgattgagg ccctgcatcg ccaggtcgtc
    22621 agcggtctga atgaagcgca gctgatgcgc gatttcgtcg ccgatggtgg ctcgctgatt
    22681 gggctggtga aaaagcattg tgagatctgg gccggtgact aagtagggaa ttgccttaca
    22741 gaagaatatc cctgacaatg gtgttttaac ttacatttaa caatgttatg aaacaccctt
    22801 tagaaacctt gactaccgca gcaggcattt tgctgatggc tttcctctct tgcctgctgc
    22861 tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact ggtgaccatg ttccatctga
    22921 tggatcttag ccagctttac actttattgt tttgtctgtg ttttttagtg ctgggcgcta
    22981 ttgagtattt tgttctgcgc tttatctggc gacgctggtt ctcgctggcg gattaaacgt
    23041 cgattatcgc cgtacttcgc ggcatacttt gcttattctc cttcgcctaa aggaacgttt
    23101 atggataagc aatcactgca cgaaacggcg aaacgcctgg cccttgagtt accctttgtc
    23161 gagctttgct ggccttttgg cccggagttc gatgttttta aaattggcgg caaaattttt
    23221 atgctgtcgt cggagctgcg cggcgtcccc tttatcaatc tgaagtccga tccacaaaaa
    23281 tccctgttaa atcagcagat atacccgagc attaagccag ggtatcacat gaataaaaag
    23341 cactggattt cggtgtatcc cggcgaggaa atctccgaag cgttacttcg cgatctgatc
    23401 aacgattcgt ggaatctggt ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt
    23461 ccaggctaaa gcggaaatct atagcgcatt tttctcgctt accatttctc gttgaacctt
    23521 gtaatctgct ggcacgcaaa attactttca catggagtct ttatggatat catttctgtc
    23581 gccttaaagc gtcattccac taaggcattt gatgccagta aaaaacttac cccggaacag
    23641 gccgagcaga tcaaaactct cctgcaatac agcccatcca gcaccaactc ccagccgtgg
    23701 cattttattg ttgccagcac ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt
    23761 aattatgtgt tcaacgaacg taaaatactt gatgcctcgc acgtcgtggt gttctgtgca
    23821 aaaaccgcga tggacgatgc ctggctgaag ctggttgttg accaggaaga tgctgatggc
    23881 cgctttgcca cgccggaagc gaaagccgcg aacgataaag gtcgcaagtt cttcgctgat
    23941 atgcaccgta aagatctgca tgatgatgca gagtggatgg caaaacaggt ttatctcaac
    24001 gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg acgcggtacc catcgaaggt
    24061 tttgacgccg ccatcctcga tgcagaattt ggtctgaaag agaaaggcta caccagtctg
    24121 gtggttgttc cggtaggtca tcacagcgtt gaagatttta acgctacgct gccgaaatct
    24181 cgtctgccgc aaaacataac cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc
    24241 ggctatttcc cctcagattc tcctgatttg cataaccctg tttcagccgt catcataggc
    24301 tgctgttgta taaaggagac gttatgcagg atttaatatc ccaggttgaa gatttagcgg
    24361 gtattgagat cgatcacacc acctcgatgg tgatgatttt cggtattatt tttctgaccg
    24421 ccgtcgtggt gcatattatt ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca
    24481 tcgccagttc acggctttgg ttgcaaatca ttacccagaa taaactcttc caccgtttag
    24541 cttttaccct gcaggggatt atcgtcaata ttcaggcggt attctggctg caaaaaggca
    24601 ccgaagcggc agatattctg actacctgcg cgcagttgtg gatcatgatg tatgcgctgc
    24661 tttcagtctt ctcgttgctg gatgtgattt tgaatctggc gcagaaattc ccggcggcat
    24721 ctcagttacc gctgaaaggg atatttcagg ggattaaact gatcggcgcg attctggtcg
    24781 gcattttgat gatctcgctg ctgattggtc agtcgccagc gattctgatc agcggtcttg
    24841 gtgcaatggc tgccgtgctg atgttggttt ttaaagatcc gattcttggt ctggtggcag
    24901 gtattcagct ttccgcgaac gatatgctga aactgggcga ctggctggag atgccgaaat
    24961 acggcgcgga tggcgcggtg atcgatattg ggttaaccac cgtcaaagtg cgtaactggg
    25021 acaataccat taccactatt cccacctggt ctctggtttc tgactccttt aaaaactgga
    25081 gcgggatgtc agcatctggc gggcgacgta ttaagcgcag tatcagtatt gatgtcacca
    25141 gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa taaagcgcat ttgttaaagc
    25201 cttatttaac cagccgccat caggaaatta atgagtggaa tcgccagcaa ggttctacgg
    25261 agtcgatatt aaacctgcgc cgaatgacca atattggaac ctttcgtgcc tatctgaacg
    25321 aatatctgcg taaccatccg cggattcgta aagatatgac tttaatggta cgccaactgg
    25381 ctccaggtga taacggttta ccgctcgaga tctatgcgtt taccaacacc gtggtgtggc
    25441 tggaatatga aagcattcag gctgatatat tcgatcacat atttgcgatt gtcgaagagt
    25501 ttggtctgcg acttcatcag tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat
    25561 ttaagcagta attaaaaaaa ccgctctcat cgaatggatg agagcggaca tcgttttgtc
    25621 ggatgtagcg tgaatgcctt atttccgacg cagcgtttta aatgccacaa acaggaatac
    25681 aatccacacc ggcagcagga tcgctgacaa gcgcatatca tccatcgtgc acatcagcag
    25741 caaaatcatg ccgaggaagg caatgcaaag atagttgccg aacggataga gcagcgcctt
    25801 aaactgtgtt tcacgcccct gacgtcgcat cgctgcacga aaacgcagat gcgccagaca
    25861 gatcataatc cagttcaaca gcagcgttgc taccaccagc gccatcagca gaccaaacgc
    25921 tttttgcggc agcagatagt tgattaacac caccagcgaa gtgatcgctc cggaaagcat
    25981 cagcgagtta atcggcacac cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc
    26041 ctgcacagaa aggccaaaca gcatgcggct gttagagtaa accccgctgt tatacactga
    26101 cagcgatgct accagaatga cgaagttcag cgcagaagct accacgttgc tgtcgagatt
    26161 atggaaaatc atcacaaacg ggctactgtt ggatttcact tccacccacg gatagagcgc
    26221 cagtaaaacc accagtgaac cgatgtaaaa cagcaggatg cgatacacca cctgatttac
    26281 cgcttttgga atgctttttt ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc
    26341 cagaccgccg aaggagaaca taattaccgc cagcgacaaa atcagcccat tccagccggt
    26401 ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg gctttctcgc cgccgtgacc
    26461 agaaaacagc agccacaggc caaagccgat cataccgatg atcgccagca ctttaatcaa
    26521 cgcaaaccag aactcggttt cgccatataa gcgcacgttc accaggttaa cggcgttgat
    26581 gataataaag aaggcggcag cccaaatcca cgttggaaca tccgggaacc agtactgcat
    26641 atagatgccc gcagcggtca gctctgccat ccccaccagc acgaacatca cccagtagtt
    26701 ccagccagag aggaagcccg cgaacggtcc ccagtattta taggcaaagt gggcaaacga
    26761 accggatacc ggctcttcga ccaccatctc gccgagctgg cgcataatca ggaaagcgat
    26821 gatcccggcg acgccgtagc ccagcaatac agccggaccc gccatctgaa tcgccgggcc
    26881 aatgccaaga aacagaccag taccaattgc gccacccagc gcaatcagtt gaatatgacg
    26941 gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac gcagtatctt ccgatacggt
    27001 tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg ttgaggggca ccttttaaca
    27061 tttagtgccc atcgtcgcaa gacacaatcc acacggttaa acggggtatc ctgcttttat
    27121 ttccgtaccc gatgtcggtg cagccacatc agcttatacg ccgccgggat aataaacagc
    27181 gacagcaaag gtgcggtgat catgccgcca atcatcggtg cggcaatccg gctcatcacc
    27241 tctgaaccag cccccgttcc ccacagaatc ggcagcagac cggcgataat caccgccacc
    27301 gtcatcgctt tcgggcgcac gcgcagtacc gcgccgtgat acaacgcctc atccagcttc
    27361 tgctcactga atgtttgcgg attattcaac gacggctcgg cctctatggc gtgacgtaaa
    27421 tacatcagca tcaccacgcc aaattcggcg gcgaccccgg caagggcaat aaagccagta
    27481 cccgtcgcca cagaaagatg aaagcccatc caccacagca accagatgcc gcccaccagc
    27541 gcaaacggta cgctgctgat aatcagcaac gcttcgccca cccgacggaa tgccagatac
    27601 aacagcacga agatgatcat cagtgtcatc ggcaccatga gtttcagctt atggttggcg
    27661 cgctccagta gctcgaactg cccggagaat gccacgctgg tgcccggttt taactgcact
    27721 ttttcagcta tcgctttttg caaatcgtga accaccgaca ccatgtcacg atcgcgggca
    27781 tcgatataaa tccagctcgt cgggcgcgca ttctcggttt tcagcatcga cggtccggta
    27841 gagactttaa cgtcggccac gtctgccagg gtgatttgct gcttcatcgg cgtcaggatc
    27901 ggcaactggc gcagtgcctg cggactatcg cgccagcttt gcggataacg cagattaatg
    27961 ggataacggg caatcccttc caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc
    28021 acaaacaact gcacatccgc caccgtcata ccgtaacgcg cggctttttc acggttaatc
    28081 tcaacgttga tatagcgccc accttccaga cgctcggcaa gcgcagaagc cacgcctggt
    28141 accgttcgtg ccacctcttc aatttgctcc gccatcgcgt cgatatccgc cagcacagtg
    28201 ccggaaactt taatgccgat ggggctttta atgccagtcg agagcatatc gatacggtta
    28261 cgaattggcg gcacccacag attcgccagc cccggcagcc gcacggtgtt atccagttcc
    28321 tcaatgattt tgtccatcgt catgccaggc cgccactgat cctgcggctt aagctggatg
    28381 gtcgtttcta ccatctccag cggagcagaa tcggtggcgg tttccgcttt cccggttttg
    28441 ccaaataccc gcgccacttc aggtacgctc ataattagct tgtcggtttt ttgcagcata
    28501 ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg gcatatacaa caagtcgcct
    28561 tcattgatct gcggtaaaaa ttccccgcca actttattga gcggccagag aaccgtcagc
    28621 accgaaagcg ctgtcaccag cagcgtggtt ttcggccagt gcagtacttt cagcaacagc
    28681 ggatgataaa cacgaatcaa aaagcgattg agcgggttac tgctttccgg cggaattttg
    28741 ccacggatcc agtaccccat caggataggg atcactacga tcgccagcag cgccgcgccc
    28801 gccatcgcat aggttttagt gaacgccaac gggccaaaca gacgcccttc ctgcccttcc
    28861 agggtgaaga tcgggataaa cgacaacgtg ataatcagca gactgataaa tagcgccgga
    28921 cccacttcaa cagatgcatc ggtgatcacc tgccagcgcg ttttattatc cagcgtggcg
    28981 tcaggatgct ggtgctgcca ctcttccagc cgtttatgtg cattttcgat catgacgata
    29041 gcggcatcga ccatcgcccc gacggcaatc gcaatgccgc ccaacgacat aatattggca
    29101 ttcagtccct ggaagtgcat gacaataaaa gcaatacaca accccagcgg caacgaaata
    29161 atcgccacca gcgccgagcg cacatgccag agaaacagcg cacagactac cgccaccaca
    29221 ataaactctt ccagcaactt gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg
    29281 cgatcgtatg ttgtaactat ctccacgcct tccggcagac tacttttcag cgtttccagt
    29341 ttgtccttca cggcggcgat cacttctcgg gcgtttttgc cggatcgcag gatcaccacc
    29401 ccgcccgcca cttcgccttc gccgtttagt tcggcaatgc cccggcgcat ctccgggcca
    29461 atctggacct tcgcaacatc gcgcagataa acgggcacgc cattttcact ggcttttaaa
    29521 acgatgtgat taaagtcgtc gagcgtttgc agatagccgc tggcgcgcac catatattcc
    29581 gcttccgcca gttcgatcga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt
    29641 tttacttcgg cgagactgat gccatactgc gccaggcgct ggggatcgat aaccacctga
    29701 tactctttca ccacaccgcc caccgacgcc acttccgcaa cgtcagggat ggttttcagc
    29761 tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg ccagatcgtg cttaccgctg
    29821 cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg tcccagctcg
    29881 gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgaa
    29941 cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga gtcgccaaac
    30001 tgtgagaaac cgcgcacagt cttcgcgcca ggcaccgaca acatggtggt ggttagcgga
    30061 taagtcacct gattttcaac gatttgtggt gcctgaccgg gatagctggt tttaataata
    30121 acctgcacat cggagagatc cggcagcgca tccactggcg tattaatgat ggtccaggtg
    30181 ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc caccgagcga
    30241 cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca ctttcagagc
    30301 gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac aggccgctgg
    30361 aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacgcct tgcgatgcct
    30421 ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta atcacccgct
    30481 gttcgctgcc ggtatcaatc agcgcttgtg acggaatgag cagcatcggt tcgctggcgg
    30541 tgttgagttg caaccaggcg ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgactt
    30601 ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc aggtagcagc gtccatttgc
    30661 ggatggtgag tgttttatcc ggtcgcgccg gaacggtgag ggtaaactgc gaggcatctt
    30721 tcaccagcca ggcgatggac tccgggatcg cagcagtgac ccacaccggg tccataccct
    30781 gaattttcgc gaccacgtta tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg
    30841 tgatcacgcc atcaatgggc gctttgagcg taaagcgagt ctggattttt tgcgtggcga
    30901 tcaggcggcg aatatccgcc tccggcattc ccgccagtcg cagccgctca agaatgcctt
    30961 cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa atactcagtc tgcgcttcca
    31021 cccagtcagg aatggtcagg tcgagaagcg gtgtgccctt ttgtacttta tcgcccacgg
    31081 taagcggata caccttgtcg ataaacccgg cagcgcgagc ctgcacaatg gcatactgat
    31141 actcgttgta actgacattc gccgggaaac tctgggcaaa agtcagcggt ccgcgcgtga
    31201 cggtagccgt tttcaccccc agattctgcg tctgagtcgg gtcaatgcgc acaccagacg
    31261 cagaactctc ttcatcggca tatttcggca ccagatccat atccataaac ggcgatttac
    31321 ctggtttatc gaaccgcgta ttgggataca tcgggtcgta ccagaataag attttacgtt
    31381 ctgcggtcga cgttttttct gcaggcggtt ccgcctttgc aacccaggta aaacctgccg
    31441 cagaaataat accgcccgcg atcatgctgc cgataataag cgcgattttt ttcatctcat
    31501 taaacctggg ttactggctg actttaatat cctgtaataa agaaaggttg ccctgctgga
    31561 caaaattaaa cgccactttg tcgccagttt taatttcact cattttcgtc tgcggagtga
    31621 tggtaaagcg catggtcatc tccggccagt tcacggcagc aatcggatcg tgatggatgg
    31681 tgattttttt gctttccaga tcaataccct ttaccacgcc agtggcgcta ataacctgcg
    31741 gttgtacttc gctcatggtt tcatgatgat gttcgttagc ctgggcatta aagccaataa
    31801 cggtaaacag actgaacatt gcgacttgca gtgctttttt catttattct ctcctggagt
    31861 taaaagttat tgctgccaac cgccaccaag tgcggtatat aaagaaattt cgttaacctg
    31921 acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca aataacgaac gttcggcatc
    31981 cagcacttcc agataactta ctgcgccgtg ctgatataat gcccgcgccc gttgcaaagt
    32041 aatttgcagc gatgccagat aacgctgctg ggcgctaatt tgatcgttca ggctttgacg
    32101 taatgccagc gcatctgcca cttctttaaa ggcgttctgg attttctgtt cataattcac
    32161 taccgactgc tgctggcgaa tttcggcgat atccagattg gcctggttgc gtccggcatt
    32221 aaaaatgggg atctcaattt taggaataaa attccacatc ccgctgctgg cgttaaataa
    32281 tgacgacaga tcgctgctgg cggttgatat tccgctggtc aggcttatag atgggaaaaa
    32341 tgccgcgcgt gcggccccaa tattggcatt agccgccatt aacgcgtgtt cagcttccat
    32401 aatatccggg cgctgcaata agatttgcga cgacaagccc gccggtaatt taacgctttg
    32461 caggctgtcg ctgtttactg tctgcgcttg cggcagcttg ccgtagcttc ccagcaacag
    32521 ttgcaatgca ttattcgcct gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt
    32581 actttctatc accccgcgag cctgttccag cgccagaaca ttactgctac ctgtcaacag
    32641 ttgtttttcg acaaacgcat atgactgctg ataattacgc agcgtttctt cagctatttg
    32701 caattgcgca tacgccagtt gctgattgaa atagctttgc gcgacattag aaaccagcag
    32761 aatatgcacc gcgcgctgag cttcctcagt ggctaaataa ttttggcgct cggcttcgct
    32821 catgttcttt aagcgaccga aaaaatcgag gtcaaagctg gcgttaaggc cggtcgaaaa
    32881 ctcccgtgtc gtggcggtat tccctttaag attaccgctc cagctgccgc tgccctcgcc
    32941 attgagctgt gggtagcggt cggcatcggt cagacgatat tgcgcccgtg cctcctgcac
    33001 tttcagcgtc gccatgcgca aatcccggtt attcaccagc gcctcgctaa tcagcgtctt
    33061 cacctgatta tcaacaaaaa aggtgcgcca gcccgcgttc tgatagttat ctgctgcgtt
    33121 aacgaggccg ttctggctga gtgagaactg ctgcggtacg ggcattgccg ggcgctgata
    33181 atccggtgcc agtgaacaac cggttagcgc aagggccaca caaaatggca gaagtttaca
    33241 aggagacata ggctcataat ttctgatgat tttatgccgc caactttact cgccaggctc
    33301 tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt gccaataagc gattgccatc
    33361 tgatcccgct actctagaat tgcccgggca acatgcggag gaaatatgaa actgttgatt
    33421 gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag ggttaaccga agccggtttt
    33481 gtggtcgatt tggccgacaa cgggctgaat ggctaccatc tggcgatgac cggtgattat
    33541 gatctgataa tcctcgatat tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg
    33601 ttacgctccg ccaataaagg gatgccgatt ctgttgctta ccgcgcttgg caccattgaa
    33661 catcgcgtca aggggctgga gttgggggca gatgactacc tgatgaaacc attcgctttt
    33721 gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg gggcggcggt aattatcgaa
    33781 agtcagtttc aggttgccga cctgatggtc gatctcgtca gccgcaaagt cacccgcagc
    33841 ggcacgcgca tcactttgac cagtaaagag tttactctgc tggagttttt tctccgccat
    33901 cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac
    33961 agcgacacta acgccattga tgtggcggtg aagcggctac gcggcaaaat cgacaacgac
    34021 tttgagccga agctgattca gaccgtgcgc ggcgtgggct atatgcttga ggtgccggat
    34081 ggtcagtaag ccatttcagc gcccgttttc gctggcaacc cgtctgacct tttttatcag
    34141 cctggccacc atcgcggcgt ttttcgcctt tgcatggatc atgatccact cagtgaaagt
    34201 gcattttgcc gagcaggata ttaatgattt aaaagaaatt agcgccactc ttgaacgggt
    34261 acttaatcat cctgacgaga cgcaagcccg acgcttaatg acgctggaag atatcgtcag
    34321 tggttattcc aacgtgttga tttccctggc agatagccac ggcaaaacgg tgtatcactc
    34381 ccccggtgcg ccggatatcc gcgagtttac gcgtgacgcc atacccgata aagacgcgcg
    34441 gggcggtgag gtgtatctcc tttccggccc gacgatgatg atgccaggcc acggtcacgg
    34501 gcatatggaa cacagcaact ggcggatgat taacttgccg gttggcccgt tggtggacgg
    34561 caaaccgatt tatacgctct acatcgcgct ttcgatcgat tttcatcttc attacataaa
    34621 tgatttgatg aataaactta ttatgaccgc atctgtcatc agcatcctga tcgtctttat
    34681 cgtgctattg gcggtacata aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca
    34741 gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg cagaccgtgc ccattgagct
    34801 ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt attgaggatg tctttacccg
    34861 ccagtccaat ttctcagcgg atatcgctca cgaaattcgc acgccgatta ccaatctcat
    34921 cacgcaaacg gaaatcgccc tcagccagtc tcgcagccag aaggagctgg aagatgtgct
    34981 ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct
    35041 cgctcaggcc gataacaacc agctaatccc cgaaaagaaa atgctcaacc tggcggatga
    35101 agtcggcaaa gtgttcgatt ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg
    35161 gtttgttggc gacaaatgtc aggttgcggg cgatccgctg atgctgcgtc gggcgttaag
    35221 caacctgctt tctaatgccc tgcgttatac gccacccagt gaggctattg tagtgcgctg
    35281 ccagacggtc aatcatcagg tgcaagtttc cgtcgaaaac cccggtacgc ccattgcgcc
    35341 cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt gacccttccc gccagcgaaa
    35401 aggtgaaggt agcggtattg ggctggcgat agtgaaatcg attgttgtcg cgcataaagg
    35461 cacggttgcg gtaacgtcag atgcgcgggg gacaaggttt gtgattatgc tgccggagag
    35521 agagtgatta ttaagttcaa attcagggcg tggtgcatca cctcctggta ttgttgatca
    35581 acaaattaga gaaaaactta ttaaacatat gaaatatcga aggcttttat ttttcaaagt
    35641 ttcacgagat gtttgtatat gaaacttagt taaatctgtg tttcttccaa ttgtagatca
    35701 caatgccctt tctaaatttt catcgttata aacaagccat ctatcatata aagcaatgaa
    35761 taatttaatc gcgattaatc atcattaatt gttatattat tcactcaatc tatttatcaa
    35821 gtgaatttga taaattccta atttaaaata tccactattg agccagtatt taacaacgtt
    35881 aagatgtgca atgtgcagat catgttatca gggtggagcg gtagcatata tttcttatgc
    35941 aatcattcat tttaataatg ttaattggat tatttattgc tgtgaataaa aatatattat
    36001 gaatattggt ttaactttgc gtttttgtta ctcgttgtga tatgcgtacc tgtcctgaat
    36061 aacgccaaga aacaagaggt tgagcttgtg attatttttt tgactgaaca gacacccgtt
    36121 tctgtaaagt aatatttaaa caaaattctc acataaccat agcattttct gttccttccc
    36181 taataacgtc tgaagtcgtc ccgcttatca cattatccgc agttgtacgg tacggatcat
    36241 tattcatttt actggaggtt gctatgtcaa ccggattacg tttcacactg gaagtggacg
    36301 gcctgccgcc ggatgctttt gcggtggttt cctttcatct gaaccagtca ctctcttcgc
    36361 tcttttccct cgatctctcc ctggtcagcc agcagtttct ctcccttgaa tttgcgcagg
    36421 tgctggacaa aatggcctac ctgacgatat ggcagggcga tgaagtacag cgccgggtga
    36481 aaggcgtggt gacctggttt gaactcgggg agaacgacaa aaaccagatg ctgtacagca
    36541 tgaaggtgca cccgccgctg tggcgtgccg gtctgcgcca gaacttccgt atcttccaga
    36601 acgaggacat caaaagcatc ctcggcacga tgttgcagga aaacggggtg accgaatgga
    36661 gtccgctgtt cagcgagccg catccttccc gtgagttttg tgtccagtac ggtgagactg
    36721 attacgattt cctgtgccgg atggcggcgg aggaaggcat cttcttttat gaggagcatg
    36781 cttacaaaag taccgaccag agcctggtgc tgtgcgacac agtccgccat ctgcccgaat
    36841 cttttgaaat cccatggaac ccgaacaccc gtaccgaggt gagcaccctc tgcatcagcc
    36901 agttccgcta cagcgcacaa atccgccctt cttccgtggt gaccaaggac tacaccttta
    36961 aacgacccgg ctggccaggg cgttttgatc aggaaggcca gtaccaggat taccagcgca
    37021 cgcagtatga agtgtatgac taccccggac gtttcaaggg tgcccacggg cagaactttg
    37081 cccgctggca gatggacggc tggcgaaaca acgcagaagt ggcgcgcgga acaagccgtt
    37141 cgccggagat atggccggga cg
//
LOCUS       DAFATA010000034        36847 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFATA010000034 DAFATA010000000
VERSION     DAFATA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.034"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(232..990)
                     /locus_tag="LGJ20_002152"
     CDS             complement(232..990)
                     /locus_tag="LGJ20_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK2386207.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(998..2101)
                     /locus_tag="LGJ20_002153"
     CDS             complement(998..2101)
                     /locus_tag="LGJ20_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK2386208.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2111..3292)
                     /locus_tag="LGJ20_002154"
     CDS             complement(2111..3292)
                     /locus_tag="LGJ20_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK2386209.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3360..4385)
                     /locus_tag="LGJ20_002155"
     CDS             complement(3360..4385)
                     /locus_tag="LGJ20_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2386210.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPAVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4816..5037)
                     /locus_tag="LGJ20_002156"
     CDS             complement(4816..5037)
                     /locus_tag="LGJ20_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386211.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5290..8394)
                     /gene="acrF"
                     /locus_tag="LGJ20_002157"
     CDS             complement(5290..8394)
                     /gene="acrF"
                     /locus_tag="LGJ20_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HBK2386212.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8406..9563)
                     /gene="acrE"
                     /locus_tag="LGJ20_002158"
     CDS             complement(8406..9563)
                     /gene="acrE"
                     /locus_tag="LGJ20_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBK2386213.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            9962..10624
                     /gene="acrS"
                     /locus_tag="LGJ20_002159"
     CDS             9962..10624
                     /gene="acrS"
                     /locus_tag="LGJ20_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBK2386214.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10627..10806)
                     /locus_tag="LGJ20_002160"
     CDS             complement(10627..10806)
                     /locus_tag="LGJ20_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBK2386215.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(10890..11774)
                     /gene="yhdJ"
                     /locus_tag="LGJ20_002161"
     CDS             complement(10890..11774)
                     /gene="yhdJ"
                     /locus_tag="LGJ20_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBK2386216.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(11860..12156)
                     /gene="fis"
                     /locus_tag="LGJ20_002162"
     CDS             complement(11860..12156)
                     /gene="fis"
                     /locus_tag="LGJ20_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBK2386217.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12182..13147)
                     /gene="dusB"
                     /locus_tag="LGJ20_002163"
     CDS             complement(12182..13147)
                     /gene="dusB"
                     /locus_tag="LGJ20_002163"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK2386218.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13476..14357)
                     /gene="prmA"
                     /locus_tag="LGJ20_002164"
     CDS             complement(13476..14357)
                     /gene="prmA"
                     /locus_tag="LGJ20_002164"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK2386219.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14369..15820)
                     /gene="panF"
                     /locus_tag="LGJ20_002165"
     CDS             complement(14369..15820)
                     /gene="panF"
                     /locus_tag="LGJ20_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBK2386220.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(15810..16052)
                     /locus_tag="LGJ20_002166"
     CDS             complement(15810..16052)
                     /locus_tag="LGJ20_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBK2386221.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16161..17510)
                     /gene="accC"
                     /locus_tag="LGJ20_002167"
     CDS             complement(16161..17510)
                     /gene="accC"
                     /locus_tag="LGJ20_002167"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK2386222.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17521..17991)
                     /gene="accB"
                     /locus_tag="LGJ20_002168"
                     /gene_synonym="fabE"
     CDS             complement(17521..17991)
                     /gene="accB"
                     /locus_tag="LGJ20_002168"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK2386223.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(18969..19943)
                     /locus_tag="LGJ20_002169"
     CDS             complement(18969..19943)
                     /locus_tag="LGJ20_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK2386224.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASHILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            20095..22035
                     /gene="csrD"
                     /locus_tag="LGJ20_002170"
     CDS             20095..22035
                     /gene="csrD"
                     /locus_tag="LGJ20_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBK2386225.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            22340..23383
                     /gene="mreB"
                     /locus_tag="LGJ20_002171"
     CDS             22340..23383
                     /gene="mreB"
                     /locus_tag="LGJ20_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBK2386226.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            23449..24552
                     /gene="mreC"
                     /locus_tag="LGJ20_002172"
     CDS             23449..24552
                     /gene="mreC"
                     /locus_tag="LGJ20_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK2386227.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24552..25040
                     /gene="mreD"
                     /locus_tag="LGJ20_002173"
     CDS             24552..25040
                     /gene="mreD"
                     /locus_tag="LGJ20_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK2386228.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25049..25642
                     /gene="yhdE"
                     /locus_tag="LGJ20_002174"
     CDS             25049..25642
                     /gene="yhdE"
                     /locus_tag="LGJ20_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBK2386229.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            25632..27101
                     /gene="rng"
                     /locus_tag="LGJ20_002175"
     CDS             25632..27101
                     /gene="rng"
                     /locus_tag="LGJ20_002175"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBK2386230.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27169..30969
                     /gene="yhdP"
                     /locus_tag="LGJ20_002176"
     CDS             27169..30969
                     /gene="yhdP"
                     /locus_tag="LGJ20_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBK2386231.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDRLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWVPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINSDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNIELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            31125..32570
                     /gene="tldD"
                     /locus_tag="LGJ20_002177"
     CDS             31125..32570
                     /gene="tldD"
                     /locus_tag="LGJ20_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBK2386232.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(32698..33627)
                     /gene="aaeR"
                     /locus_tag="LGJ20_002178"
     CDS             complement(32698..33627)
                     /gene="aaeR"
                     /locus_tag="LGJ20_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBK2386233.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            33810..34013
                     /locus_tag="LGJ20_002179"
     CDS             33810..34013
                     /locus_tag="LGJ20_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBK2386234.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            34021..34953
                     /gene="aaeA"
                     /locus_tag="LGJ20_002180"
     CDS             34021..34953
                     /gene="aaeA"
                     /locus_tag="LGJ20_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBK2386235.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            34959..>36847
                     /gene="aaeB"
                     /locus_tag="LGJ20_002181"
     CDS             34959..>36847
                     /gene="aaeB"
                     /locus_tag="LGJ20_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBK2386236.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQA"
BASE COUNT         9095 a         9314 c         9534 g         8904 t
ORIGIN      
        1 catcaaataa aacaaaaggc tcagtcggaa gactgggcct tttgttttat ctgttgtttg
       61 tcggtgaaca ctctcccaac caggacaaat ccgccgggag cggatttgaa cgttgcgaag
      121 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      181 cgaaaggcca tccgaaagga tggccttttt gcttttcgaa ctaacattca attaatggat
      241 tacctgcgat aaaaatgccc tcgtacgctc tgatttagga tgcgcaaaaa attcatcagg
      301 tgcagcttgt tccactatct ccccacgatc cataaaaatt acccggtcag cgacggtacg
      361 tgcaaacccc atctcatgtg ttacacacaa cattgtcata cccgactgcg ccagcccaat
      421 catcgtatcc agcacctctt tcaccatctc cggatcgagc gccgacgttg gctcatcaaa
      481 caacataatt ttcggcttca tacacagcga acgcgcaatg gcaacgcgtt gctgctgacc
      541 accagaaatt tgtccgggaa acttatgcgc atgttcggca attctcaccc gctcaaggta
      601 atgcatcgcc agagcttcag cctctttctt tggcatcttg cgtacccaaa tcggtgccag
      661 ggtacagttc tgtagaacgg tcagatgagg gaagagattg aaatgctgaa agaccattcc
      721 cacttcctgc cggacgcgct caatattgcg gatatcttca ttaagttcga tgccatctac
      781 cacgatccgt ccctgttgat gttcttccag atgattaata caacgaatgg ttgtcgattt
      841 accggaacct gaagggccac acagaacgat ccgttctccc ggttgcacgg ttaaatttat
      901 atttttcaaa acatggaatt gtccatacca tttattgacg ttttccagcg taatcatcgc
      961 gttagcaggt tgcagtaaaa tttggctcat agtgtcctca atgcggtgta cgcccggtgt
     1021 taaaacgttt ttccagatgc tggctatagc gcgacatgct gaaacagaag atccagtaga
     1081 tcagcgcggc gaaaacatac ccttccgtcg acatacccaa ccaggtggga tcaacggttg
     1141 cctgctgaac gctactgaaa agatcgaaca acccgataat gatcaccagg ctggtatctt
     1201 tgaagagtgc gatgatggta tttaccagcc caggaattac cagcttcaac gcctgtggca
     1261 gaataaccag cccctgagtt ttccagtaac ccaacgccag cgactctgcc gcttcatatt
     1321 gccctttagg cagcgcctgt aatccgcctc gcacgacttc cgcaacatat gctgactgaa
     1381 acaggatcac gccaaccagc gcgcggatca gtttgtcgat attggtgcct tctgccataa
     1441 acaaaggcag catgaccgaa gacataaaca atacggtaat cagcggtacg ccgcgccaga
     1501 actcgataaa aatgaccgat aagatacgca caatcggcat atgggagcgg cgacctaacg
     1561 ccagtaagat cccccacggt agcgcccccg caatcccaac ggatgcaata attaaagtta
     1621 gcgtcagccc gccccattgc cgggtttcaa cccgctccag accaaaaaaa ccgccataca
     1681 tcagccacca gacaatcagt gggtaaatca ccgcccagac cgcaatatag cgaccgcgat
     1741 gcgggagtat tttccagaac attggtgcga ttgacacaag gccaatcagt aatgccaggt
     1801 taattcgcca gcgttggtcg tgtgggtaaa gtccatacat aaactgacca aatcgttcgt
     1861 ggatgaagac ccaacaggca ccggcttttg tgcagtcggc acgcgttgag ccaacccaat
     1921 tagcctgcaa aaatgcccag ttcagcaacg gtgggatcag ttcccacatc aaccatatgc
     1981 agccaatagt cagcaggcta ttgctccagc tggagaacag attttttcgc acccacacca
     2041 tcgcgcggct tgagttatgg ctcgctgggc gcgagggctg agacagcaat acttttgtca
     2101 tcatgactcc ttagcgttca acgatcgcga tgcggcggtt atagatattc atcagcagcg
     2161 agatagtcag gctgataatc agatagaccg acatggtcat ggctatcgtc tcgatggctt
     2221 gccccgtctg gttcagcacg gtgccagcaa acagcgaaac catatcggga tagccaatag
     2281 cggcggcaag agaggagttt ttgacgatgt tgagatactg gctggttaac ggcggaataa
     2341 tcactcgcag tgcctgggga ataatgacct ggcgtagcgt aaccgggttg ggtaatccca
     2401 gtgaccgagc cgcttcatgt tgaccataag gcactgcctg gatcccagcg cggataatct
     2461 cggcgataaa tgcagatgta taaaccgaaa gtgccagcgt taaggctgcc agttcaggaa
     2521 ttaaaaccat cccgccgcgg aagttaaagc ctcgtagggc tgggacatcc cagtgtagtg
     2581 ctgcgccaaa aagccattgc gccagtaaag gcaaaccaat gatcaacact gcggcgatcg
     2641 gccaggtgcg gcgcagttgt ccggtcttta tctggtatgt tttattaaag cggaatagcc
     2701 caacagaaag gactatagcc ataacaacag ccaggataaa cgcaataaat ccgtctccca
     2761 gctgcggtga cggaatataa agcccacgat tgctcaaaaa ggccagatca aacgcgctca
     2821 cagcctggcg tggtccgggc aaattgcgca acacggcaaa gtaccagaag aagatttgca
     2881 gcagcggggg aatattacgg aagatctcaa tataaattgt ggatagtttt cgtagcagcc
     2941 agttatccga aagtctcgcc agaccgataa agaagcctaa aacagaagcg aacacaatac
     3001 acaatgcaga aaccagtagc gtattgagta agccgacaat aaaaacgcgt ccgtaggtgt
     3061 cgccctgctg gtaatcgatc aaatgctgga caataccgaa gccagcgccg cgatccagaa
     3121 aggcaaaacc tgaagtaatg ccacgattat tgagattcgt tacagtgttg tgaaataacc
     3181 aaccaacaat gccgacaaca gcaacaacgg caaggatctg gaataaccag gcgcgaaccg
     3241 tagggttggc aaaagagagt gagcctttaa cggttgagcg gcgatgagac atagaaacct
     3301 cggaaagcat aacgctgaag agagcgccgc cgtagcgacg cccgttacct cacaccgact
     3361 taacgcacgg gcggcgcgta ctgaataccg ccgttattcc agagattatt ttgcccacgt
     3421 ttaattttca gcgggctttc tgaacctacg ttacgctcga aaatttccga gtagttaccc
     3481 acctgtttga tgatgttata ggcccattta ttatccagct tcagatcctt gccgtaatcg
     3541 ccttctttac ccagcagatg tgccatatca ggcgttgctg gattagccgc tttttcatcg
     3601 acgttctggg aattgatgcc catctcttca gcattcagca tggcgaaaag cgtccagcgt
     3661 acaatcgaga accattcatc atcgccacga cgaactgccg gcccaagcgg ttctttagag
     3721 ataacctccg gtaagacaat ccattcagct gggttgctta atttgatgcg cagggcatac
     3781 agttgcgact gatccgaggc cagcgtatcg cagcgaccag attccagtgc cttcgctgat
     3841 tcgtcagagc gatcgaaagt caccggtgtg tacttcatat tgtttgcctt gaaatagtcg
     3901 gcaacgttga gttcggtatc agtacccgcc tgaatacaga cggtagcgcc atccagttct
     3961 ttcgcgcttt ttagccccgc tttatcgtgc gtcaggaagc caatgccgtc gtaataagtg
     4021 acgccggtaa atgccattcc catcccggca tcgcgagatg aagtccaggt cgtattacgg
     4081 gagagcaaat ccacttcccc tgactgtaaa gcggtgaagc gttcttttgc tgtgagtggg
     4141 gtatatttca ctttcgtgtc gtcaccaaat acagcagcgg caacaccacg acaaacatca
     4201 acatcaatac ctgaaaactt accgtctgca tcggcataag agaacccagg taatccatca
     4261 ctgatcccgc attgcacaaa accttttttc tgaacggcat caagcgtcgc gccagcatgc
     4321 gcctgatttg caacggcaag cagcacgctg gcggcagcca gtgtggctat catcatcttt
     4381 ttcataatgc ttcctgtgag gcgaaaatta tcgttatagg acgtttggga aacgtctttt
     4441 acctgtctgt ggccatcctc ttataagcaa aagtagtgcc agcttttgcg cccgatttcg
     4501 cgtcgcaata agctgagtgt agacaggaaa taataaaaac agcgccccgc aatggtgcga
     4561 aaatgaaaca tgcgccacat tttggcgcaa aatatttacg cgtgttttac taagcagaat
     4621 aatgattgtt agcgtgagta tatttctggt gggaagtgtg aaagttaaat tgatgcgtta
     4681 tttcgaaagc agcgaaataa cgcgatataa catgctaaat cagcagagaa ttagccagca
     4741 gaatatagca atattttatc ttatgtaaag atactgtatc gttaaacaga gcgcgaaact
     4801 ccgcgccctg ttcagttatt tcgacagagc gatgatcccc atcgtcaggc cagcaacagc
     4861 cgctgccccg ccagcaatag ccccagccgt ttcccagtta tcctgagtcg tgcctttcat
     4921 acaggtattg gtgtgaacca gacgaccctg atcgtcataa accggaacgc agggcgaatc
     4981 gtgagcacag cccgccagca atgcggtgag caatgccaca ggaattaatc ttttcatggt
     5041 gttacctcag taataaacgc taacgcttaa taagttaact ctcttattaa cgagctgaat
     5101 gccattttat caccggaggt tataaatctt gcggaatgaa taatgacaaa ttatgtgaat
     5161 tgtaaaaata gtgaagggag cgggaactat agaaagcttt taaagagtga gcagaagaat
     5221 atcttattaa tttttatggg tttcactgga aataataaag gcacccgaaa gcgcctttat
     5281 gtttctgatt tatcctttaa agcaacggcg gatcaccaca aagaacaccg gtacgaagaa
     5341 gattgccagc aacgttgcag agaccattcc tcccattacc ccgataccca cagcgttctg
     5401 cgcgccactg ccggcaccgt tactgatagc tagcggtaat acgccgagaa taaaggcgag
     5461 agaggtcatc aggataggac gcagacgcat acgtactgcc atcagtgtcg cttcaacaac
     5521 acctttaccc tctttctcca tgagatcttt agcgaactca acaatcaaaa tagcgttttt
     5581 ggccgacaag ccaattgtcg ttagcaagcc caccataaag tagacgtcat ttttttgatt
     5641 aaagagtgtt gccgccagta gcacgccgac aatccctaac ggcactacca acataaccga
     5701 gacaggaatt gaccagcttt catagagtgc agcaaggcac aggaaaacaa ccacaaagga
     5761 aattgctacc agagcgggag cctggtttcc cgataagcgt tcctgatacg acatacccgt
     5821 ccagtcataa ccaatgcccg caggtaattt tgacgcaagg ttttccatca acgccatggc
     5881 atcgccggaa ctggttcctg gcgcggcttc cccctgaatc tccattgacg gcagaccgtt
     5941 gtagcgttcc agtcgcggag agccatacac ccaatgtgaa gtggtaaagg ccgagaatgg
     6001 caccatttcg ccgttggcgc tgcggacata aagtttatcg acatcttctg gcagcatacg
     6061 gaatttggca tccgcctgaa catacacctt tttcacgcga ccacggtcga tgaagtcgtt
     6121 aacgtaagtc ccacccaacg ccgttgaaat ggtctgattg atgtcagaaa gtgaaacacc
     6181 taatgcctgc gccttttcct ggtcaacttc cagtttaaac tgcgcggtgt cttccaggcc
     6241 attaggacgc acgctgacta agctggcagg atgttgcgcc gccatgccaa gcaactggtt
     6301 acgggcctgg gtcagggcat cgtgacccag cccagcctga tcaattaact caaagtcgaa
     6361 acccgttgcc gtgcccagtt caacaatggc tggcatattg aatggaatga caaaaccgtc
     6421 gcggatcttg cccaattcca ttttggcacg atggattacc gcttccgcac tgttttcatc
     6481 actattacgc tcttcccacg gtttcagact gacgaaggcc ataccggcgt tttgtgcctg
     6541 gccgctgaag ctaaagccgt taaccgtaaa gacactttca acgttcgctt tctcgttctt
     6601 cagatagtaa tccgtcactt gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc
     6661 gggtaactga atcatggtca gaaagacacc ctgatcctct tcaggtaaga aggaagacgg
     6721 aagacgtaaa aacaacacca ccattcctgc aacaatcagc gcatagatca gtaaatatcg
     6781 tcctgtggaa ccgaggattt tgccgacgct gttggtgtag tggttaacgc tatgatcgaa
     6841 ggtggtatta aaccagccga agaaaccacc cttattttcg tgatgctcag ctgaggtggg
     6901 tttgagcagt gttgcacata acgcaggggt aagaatcaat gccaccagaa cagaaagcgc
     6961 cattgccgaa acgatggtga tagagaactg gcgataaatt gccccagtag aaccgccgaa
     7021 gaatgccatc ggaataaata ccgctgacag caccatcgcg atacccacca gtgcgccctg
     7081 aatttgcgac atcgattttt ccgtcgcttc ttttggcggg agcttatcct ccatcatcac
     7141 gcgctcgacg ttctccacca ccactatcgc atcatcgacg agcagcccga tggcaagcac
     7201 catcccgaac atcgttagtg tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt
     7261 ccctaataac accacgggta ccgcaatggt ggggatcagc gttgctcgca tattctgcaa
     7321 gaacagatac atcaccagga acaccagcat aatggcttcg aacagcgttt ttaccacttc
     7381 gtgaatagaa agctggacga atggcgtggt gtcataaggg tagagaacct tcattccctg
     7441 cgggaagaat ggctgtaatt ccgccagttt tgccttaatg gctttcgcgg tatcgagagc
     7501 attcgcgccg gttgccagct taatccccag gcccgccgcc ggttttccgt tgatacgagc
     7561 gataacgtta tagttttcac cgccaagttc aactcgtgcg acatctttca ggcgtaccac
     7621 cgagccgtca ctgtttacgc gcagggttac tttgccgaat tcttccggat tttttaaccg
     7681 cgtctgagca ataattgagg cgttcaactg ttgccctggt aacgctggcg ttccgcccaa
     7741 ctgtccggca gcgatctgat cgttctgtac cttcaactgg ttaatcacat caaccggtgt
     7801 cagtttatat ttgtttagca gatcggcgtc cagccagata cgcatcgcat actgtgcgcc
     7861 gaaaagctgt acgtcaccga cgccattcag acggctaagc gtatctttaa cgttagaggc
     7921 cacatagtcc gagatatcgt cctgtgtggt gtctgggtta tcagagacaa agcccgccac
     7981 catcaaatag ctgctactgg acttttcaac actgatcccc tgctgctgaa cctcctgcgg
     8041 cagcaacggc gtggcgagct ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc
     8101 ggtcccggac tggaaggtaa gggtaattgt cacgctaccg gcggaatcgc tggtggagga
     8161 catatacatc aggttatcga taccgttcat attctgttcg ataacctgcg tcaccgtatc
     8221 ctgcacggtc tgcgcatcag cgcccggata gtttgctgaa acagaaaccg caggcggtgc
     8281 aatggttgga tactgagcga cgggtaattg taggatcgcc agtgcgcccg ccatcatcag
     8341 aataatggcc agcacccatg caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga
     8401 ttaccttact tcgatgcagt atctgcgggg gtatcggtag tggctttaac ctgctctccc
     8461 ggacgcgctt tttgcaggcc gctgacaatg acttgatcgc cagatttcag accttcgcta
     8521 atcaaccatt tatcgccaat cgcctgactg gcaacgacag ggcgcgcttc cacctgactt
     8581 ttatcgttaa caatcagcac ggttgcatca ccacgcggtg tgcggctaac gccttgttgc
     8641 gggataagaa tggcgtcagg ttggacgcct tcatcaatcc gtgcgcgcac aaacataccc
     8701 ggcaaaagcg tatgttgcgg gttagggaag acggcacgta gggttatgga gccggtgctt
     8761 tcatcaacgg tcacatcaga gaattgcagc gtacctttca ggggataggt ttgaccgttt
     8821 tccatgacca actctacgtt gctggtggcg ttttccttat gcaaatttcc ttgctctacg
     8881 gattgcttca gcctcataaa atcgttgctg gattgggtca catcaacgta gataggatcg
     8941 agctgctgga cagtcgccag ttcagtcgtt tgcccattag tgacaagagc gccttcggtc
     9001 acggtcgatt tgccgatacg tccgctaatt ggcgcagtca ctttggtata agcgagattg
     9061 atgcgagcgc tttcgactgt ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca
     9121 tcagcaatgg cctggtcgta ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg
     9181 taacgtttta ccgtcaaatg cgcgatggcg gcggcggctt cacttttcgc cagttcgcct
     9241 ttcgcgctgt cataatttgc ctgataggtc gcgggatcga tctggtacag ggactggcct
     9301 gcttgtacat cgctgccttc agtgaaatta cgattcagta cgatcccgct aacctgtggg
     9361 cgaacttcgg ctatacggta agcattggtg cggcctggta attcagtctt aacttctaac
     9421 ggggccgttt tcacaatatg aacggtaacc tgcggttcac cgacgtgagc tttctcttct
     9481 cccttatcgt tacaaccggc gattaaagcc gcggagatca gaataaagga gggcaggagg
     9541 aaaaacctgg catgtttcgt cattactatt cctcaaaaaa ccaaaagcgc gttatttacc
     9601 cagaaaggca acgcgttaac tcgcagaaag aaaaatacag tttgctatcc tacaaattat
     9661 cattcgtcga tgtaaggaat agttatgaat actggcgtct caaggcacat aaacacaaaa
     9721 aaagattaat attctactgt tttattttga cgcgggttga aagaggcaga attaaaacct
     9781 cgtaaattga aatatatatt catgtggcga atgtatttta ggtaaataat atatattatt
     9841 tatttgacga tttatagcaa ctcaatatta gcctctcgta taaatacaca ttaggtgata
     9901 gattaacctt cgctattttc tcactctgtg tcgaatatat ttatttcctg aataattaat
     9961 catggcaaaa agaaccaaag ccgaagctct gaagacccgg caagaactga ttgaaactgc
    10021 catcgcccag tttgcgcagc atggcgtaag caagacgaca ctcaacgaca ttgccgacgc
    10081 tgctaacgtt acgcgtggcg ctatctactg gcacttcgaa aacaagactc aactgtttaa
    10141 tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc caggaacact tgacggctgg
    10201 attagagcat gacccgtttc aacaattgcg tgaaaaattg attgtcggct tgcaatatat
    10261 tgccaaaatt ccccgccagc aggcgttgct gaaaatctta tatcacaaat gtgaatttaa
    10321 tgatgagatg ctggccgagg gagtgatacg cgaaaagatg ggcttcaatc cgcagactct
    10381 ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta gcaaataacc tcgatttaga
    10441 tgttgtgatg attattatta atggtgcctt cagcggaatt gttcaaaact ggttaatgaa
    10501 tatggcgggt tatgatcttt ataaacaagc ccccgctctg gtcgataacg tattaagaat
    10561 gttcatgcca gatgaaaaca taacgaaatt aattcatcaa acgaatgaat taagtgtcat
    10621 gtaaattcac attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt
    10681 aactgagttc atattgcggc atttgtctgt ttctgttgcc agcagttcaa tccactgcat
    10741 cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg
    10801 aatcattacc tgagcctgtg tatgtttaac caagtctggc aggaaatata cacgaaatga
    10861 ataatctgaa aaccaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg
    10921 cttaaccggc ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct
    10981 tccgcagagt aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg
    11041 ttgatctcaa taccaatgaa ttttcgcccg ctggagacgg ctacggcacc ggtagtaaag
    11101 ctaccggcaa acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt
    11161 ttcagtaagg cttccggttt ttgcgtcggg tggttctcat attcatccat caaataacgc
    11221 acgcgcggaa aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga
    11281 tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca
    11341 tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg
    11401 gagccgtagt gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt
    11461 ttgatggtaa aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtgctgttc
    11521 atgatgtaca tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac
    11581 agccagtcga taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg
    11641 atgttatatg gtgggtcggc aaagatcaga tcgacacttt cggcggggat ctttttaagt
    11701 tcggcaagcg catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca
    11761 catcctgttc tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc
    11821 gagtagcgcc tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt
    11881 caatttttta cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt
    11941 accacgggtg tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag
    12001 ctcatagagg tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg
    12061 tttaaccgag tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac
    12121 ggtcagtacg tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg
    12181 tttacgcaaa attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa
    12241 tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa
    12301 cgtgtttacg tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa
    12361 cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag cgggggcagc aactccccag
    12421 tgtccagata atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta
    12481 tcatcagggc atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa
    12541 tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt
    12601 actcagcttc tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa
    12661 tgccacagtc ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc
    12721 cggtgcgaat cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg
    12781 atttaacgac atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt
    12841 tagccgggca acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg
    12901 ctgcatctgc catttctttc ggatcgctac cagcaatttg cacggtgcga ataccgggtt
    12961 catcaatgtg caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag
    13021 acatcatctc ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc
    13081 tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga
    13141 tgcgcatgag ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt
    13201 ttgcacgaga tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat
    13261 ttaaaatgag cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact
    13321 aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt
    13381 tggcacgcaa atttgcgtaa ataaccggca aatttacgtg ccttttgtga gcttgctcgc
    13441 acttcgcccc gcgtcaccct acggcgatgc gaaggttaat tcttacgacc ggtaatacgg
    13501 caccactctt ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa
    13561 acgctctctg cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc
    13621 aggacgctga ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc
    13681 acgtcggctt tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa
    13741 acgccattac gttcggcgtt gtcgcggctg gcctgaatcg cctgcggatc gatatcaata
    13801 ccaatggctt ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag
    13861 ccaaagtcga tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga
    13921 gaggtggttg gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg
    13981 gcgttttcgt ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc
    14041 atcgggtgga aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt
    14101 ttatgcgcga agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc
    14161 atatcggttt cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg
    14221 cccggcagcg gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca
    14281 cccgcttcca tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc
    14341 agttggatcc aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg
    14401 ggacggatgt accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg
    14461 gcacaatagg gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca
    14521 cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac
    14581 ccagcaccag cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc
    14641 agatgatcat ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg
    14701 taattaccgc cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat
    14761 tcagatagag atctttaatg atcgtagcgg aactttgcag caactgggca ttgatcgtcg
    14821 acatgatcgc agccataggt gcagccagga agattccggc agcaaacggt ggcagcactt
    14881 ttaccattaa cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac
    14941 ctaacgctcc ggccaggtgc ataccgaaca tcaggattgc gactacaatc gtaccgatga
    15001 tgatcccccg gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg
    15061 gcaggccaat cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca
    15121 gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta
    15181 ctgcgttgct taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa
    15241 tcagcatcac aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg
    15301 cggtatataa cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg
    15361 ccgcggtttc cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg
    15421 caaccagcaa actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca
    15481 gcatatcgtt cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga
    15541 gaataccgag tgaaagccag actgcaggaa gctgaatcat cgccagcaat acccagccca
    15601 gcccgtattt ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg
    15661 cggtgagcgt catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat
    15721 taaggaaggt gccggtgctc cgtttacgca tcgcataaac agagataccg aacaccacca
    15781 ccagataggc gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata
    15841 tcgcgataga taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg
    15901 atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag
    15961 taagcggcta ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc
    16021 tctttatggg cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg
    16081 aggattgtac cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc
    16141 cttttgacgc ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg
    16201 atgttagtgc caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg
    16261 ttggttttga taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg agcaatcgcc
    16321 acgtcacggt tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt
    16381 acggtgtagc ccgcgtagat atgagactcc caacgcactc caaaaccgcc aggtgcgtgg
    16441 aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga
    16501 cattccaccg catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg
    16561 gcagcgatac gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg
    16621 tgttctacct gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg
    16681 aactcgaaag tacctgcacc gcgatagccg atatcaacac acgctttagc acaacgttcg
    16741 ccgatgtagc gacgcagttc cggtgtaatt cccggtgctg gtgcttcttc gaccactttc
    16801 tggtgacgac gctgcataga gcagtcacgt tccgccagat agatagcgtt gccctgaccg
    16861 tcagccagta cctgaatctc gacgtggcga ggattttcca ggtatttctc catgtaaacc
    16921 atatcgttat tgaatgcggc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt
    16981 tcagcgtcgc cgcgcactac acgcatacca cgaccgccgc cgccgccgga ggctttgata
    17041 atcaccggat aacctatgcg tttagcaatg gcacggtttt tatccatatc gtcgcccagc
    17101 gggccgtcag aacctggtac gcaaggaacg cccgcttttt tcatcgccgc gattgccgac
    17161 actttgtcac ccatcaggcg aatagtgtct gctttcgggc caatgaagat aaagccggag
    17221 cgttcaacct gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt
    17281 gctactgcgc cggtgatttc agcggcgctt atgattgccg ggatgttcag ataacttttt
    17341 actgacggag cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg
    17401 cgatccgcgc tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga
    17461 atacgcaatg caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg
    17521 ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat
    17581 tgctttcacg gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac
    17641 gatgcacagg gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc
    17701 cgggcttggg gtgcggtaga aagtaccaac catcggggag cgtacgatgt gaccactgat
    17761 ttccgctgct gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg
    17821 agctggctgc tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc
    17881 aggagctgca cggctgatgc gtactgactc ttcgccttcg gagatttcca gttcggagat
    17941 gcctgattct tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt
    18001 ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg
    18061 ccccgacagc tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc
    18121 cccagagtgt caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg
    18181 caaaaaacaa aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgt
    18241 gtcagggagg acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg
    18301 gtcttatcag ggaagataat caacagctaa catgtaaata accttcaaca ccgtgtaatt
    18361 tgcaacaagc cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt
    18421 aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc
    18481 acagaccaca aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc
    18541 ggttgctggg gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca
    18601 cgcaaaatca accgactgac aattctcagc attaaatagg ttcgagtggt taatttgtag
    18661 tcaaataaaa tcagattatt caccccatgg gaccagaaat cagtaatagg ttaagcgtag
    18721 tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg aggcatatca ataatggctg
    18781 tttagaggag tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt
    18841 ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct
    18901 tttcccgtta accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg
    18961 ctaaatggtt agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc
    19021 tcggcaaagt tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat
    19081 tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt
    19141 actgaatcca ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc
    19201 agagtaaaac cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc
    19261 agcacttttg ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt
    19321 tccagaggac gggattcggc aaactcatca cgagggagaa tatgactggc acctaaactt
    19381 ttcaggtatt catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta
    19441 tgcagcagcg ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc
    19501 ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa
    19561 ccggcagtac cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc
    19621 cagtcacctt tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc
    19681 cagccagtga gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca
    19741 gttccggcaa aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg
    19801 gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt gaacatcaac cgtgacatcg
    19861 ccctccggca ggcgactttc gtcaagtgtc tgtaccgatg cgagagtttt gccgtcctgc
    19921 tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat
    19981 ttctgaagac tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag
    20041 ataaatctga tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga
    20101 ttaacgacga aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact
    20161 ttgctgggct gttcgctaag tttctacaac gcaattcagt ataagtttag tcatcgcgtt
    20221 caggcggtgg cgacggcgat tgatacccac cttgtgtcga atgacttcag cacattaagg
    20281 ccacaaatta ccgaattaat gatgtcggca gatatcgttc gtgtagacct gctccatggt
    20341 gataaacagg tttataccct ggccagaaat ggtagttatc gtccggttgg ctccagcgat
    20401 ctgtttcgcg aactgagtgt tccgttgata aagcatccgg ggatgtcgct gcgtctggtt
    20461 tatcaggatc cgatgggcaa ctatttccat tctttaatga ctaccgcgcc gctcacgggg
    20521 gcgattggct ttatcattgt tatgctcttc ctggcggtac gctggttaca acggcaactt
    20581 gccgggcaag aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct
    20641 aatgtgttgg gaaccatcta tgaatggccg cccagaacca gcagtgcgct agatacgctg
    20701 cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct
    20761 tatgccgccc aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta
    20821 gcaacgttac tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt
    20881 ctgccggatt tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc
    20941 ttcactctga cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg
    21001 cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag
    21061 agcatcgccg gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat
    21121 cgcgacgata tgatccacat tggtatctgc gcctggcgta gtggtcagga taccgagcag
    21181 gtaatggaac atgcagagtc tgccacgcgt aatgcgggat tgcagggcgg caatagctgg
    21241 gctatttacg atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt
    21301 atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc
    21361 gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt
    21421 agctcggcgg agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt
    21481 ctgcaaatca gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt
    21541 caggttaccg ttgagtcgct gattcgcccg cgttttcagc gttggctgcg cgatacgtta
    21601 atgcaatgtg aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta
    21661 ggtcaacata tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg
    21721 gtagccgtca accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat
    21781 gttgagttac tcaagctcca tccggggctg gtcagaaaca ttgagaagcg aacggagaac
    21841 cagctgctgg ttcaaagcct ggtggaagcc tgctccggga ccagcaccca agtttacgcc
    21901 accggtgtgc gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg
    21961 caaggggatt ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa
    22021 agatactcgg tttaacctgc cgtttaatct gttttcacgt agaataacgc gcgctgcgtc
    22081 tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc
    22141 ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg
    22201 cagagatttg taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg
    22261 cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct
    22321 ttcaggatta tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc
    22381 cattgacctg ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa
    22441 tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc
    22501 agtaggtcat gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg
    22561 cccaatgaaa gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt
    22621 catcaaacaa gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt
    22681 accggttggc gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg
    22741 tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc
    22801 ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc
    22861 tgttatctcc ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt
    22921 cgacgaagct atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac
    22981 cgcagaacgt atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat
    23041 cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa
    23101 cgaaatcctc gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc
    23161 actggaacag tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac
    23221 cggtggtggc gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc
    23281 agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga
    23341 aatgatcgac atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg
    23401 aaatgtctgt ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt
    23461 tttagccgtg gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc
    23521 attattattg ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc
    23581 gccgtcagtc ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcgtatcg
    23641 cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg
    23701 ttgctgaaaa acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg
    23761 cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc
    23821 tccacggtta acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc
    23881 gtttatgaag gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc
    23941 gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag
    24001 gtgctgcgca acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag
    24061 cttgagcatc tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt
    24121 ctgggtggtc gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat
    24181 acccagcgcg cttatactgt gattcaggcg cgtccgactg caggactgca acgtttgcgt
    24241 tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag
    24301 gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca
    24361 gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag
    24421 caaccgacga caggaaatgc agctactgcg cctgctgcgc ccacacagcc tgctgctaat
    24481 cgctctccac aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg
    24541 ggagggcaat agtggcgagc tatcgtagcc agggacgttg ggtaatctgg ctctctttcc
    24601 tcattgcgct gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa
    24661 actgggtgtt actcatcttg ttgtattgga tcctggcctt gcctcatcgc gtaaatgtgg
    24721 gcacaggttt tgtgatgggt gccatactgg atctgatcag cggctcgacg cttggcgtac
    24781 gcgtattggc gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca
    24841 acctcgcatt atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta
    24901 ttgttttctg ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga
    24961 gtagtgtagt caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc
    25021 agcagtttgc agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc
    25081 gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacaggcat
    25141 tgaggagcag cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcgcgcga
    25201 gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc ccggtgctgg gtgcggatac
    25261 tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc gacgcagagc atgcggcgca
    25321 gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga
    25381 cagccagcac attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga
    25441 cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg
    25501 tattcagggg ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt
    25561 cggcttaccg ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa
    25621 aagggataaa catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg
    25681 cgtatattga tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa
    25741 tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt
    25801 ttgtagatat tgggctggat aaagccgcgt ttcttcatgc atccgatatc atgccgcaca
    25861 ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg
    25921 ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg
    25981 cgcgcctgac caccgatatc acgctgcctt ctcgctatct ggtgtttatg ccaggggctt
    26041 ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga acgtgaacgc ctgaaaaaag
    26101 tggtcgcaga gtattgcgac gagcagggcg ggtttatcat ccgtaccgca gcggaagggg
    26161 ttggcgaggc tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta
    26221 tggagcgtaa aaaacgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc
    26281 agcgtgttct gcgtgatttc gccgacgccg aactggaccg cattcgcgtt gactcacgcc
    26341 tgacttacga agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc
    26401 tggagcatta caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc
    26461 agcgagcgct ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga
    26521 ccgaagcgat gaccaccgtg gacatcaata ccggggcgtt tgtcggtcat cgcaatctgg
    26581 acgacaccat tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt
    26641 tgcgcaatct gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc
    26701 gccgacgcgt gctgcactcg ctggagcagg cattgagcaa agaccgggtg aaaactagcg
    26761 ttaatggttt ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca
    26821 ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg
    26881 tggaaacggt atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact
    26941 ccgaccgttt cctggtctat gcttctccgg cagtagctga agccttgaaa ggcgaagagt
    27001 cacactcgct ggcggaagtg gaaattttcg ttggtaaaca ggttaaagta caaattgaac
    27061 cgctctataa ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat
    27121 ccggcaaagg gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg
    27181 ccggggattt tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc
    27241 ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc cggaaatcct caacaaaata
    27301 gaatccgcga ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt
    27361 ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg
    27421 gttaaacgcg ttactctggc gctggatgtc tggcagagct tgttacatat gcgctggcag
    27481 tttcgcgacc tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt
    27541 ggtggtaatg atagactgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac
    27601 catttcgatc tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag
    27661 ctggcgatcc cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg
    27721 gtaagcctct ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc
    27781 gatgatgagg ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg
    27841 aagccgtggc tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc
    27901 cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa
    27961 cagggcggtg ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg
    28021 accgcgcata ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc
    28081 acgatggacg gcaaaccctg gccgagcgga gcattgacgc tggcctgggt accggaacag
    28141 gacgttggcg gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc cagtaatctg
    28201 gagctggcag gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt
    28261 gatgtttggc gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg
    28321 cttcaggcgg cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa
    28381 tggaaattat taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat
    28441 ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg
    28501 ccactggaaa tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc
    28561 cagctggatg ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt
    28621 cgttacctgc aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc
    28681 gatgatggtt cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt
    28741 gattacttaa gtggcgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt
    28801 ggcaatccgc aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg
    28861 ctgcgcaacg cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt
    28921 gaactggact ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc
    28981 gtgcgcgcga gtaatctcac cgcagtgatc cctgactact cgaaagaaaa actgctgatt
    29041 gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg
    29101 aaagattctc tgggggcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc
    29161 ttacatcttg atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg
    29221 cgtaataaca gtctgtttat caaaccactc gacagcaccc tgaaaaattt gagcggtaaa
    29281 ttcagcttta tcaatagcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag
    29341 ccgttgaacg tggatttttc caccaaagaa ggggcaaaag cctaccaggt agcggtaaac
    29401 ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca
    29461 ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggt
    29521 gcgacctata acatagagct gaacggcgat ctaaagaatg tgagcagtca cttaccttca
    29581 ccgttagcca aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc
    29641 aacagctttg aattaaccgg acaggctggt gcggataatc atttcaatag ccgctggttg
    29701 ctcggtcaaa agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg
    29761 ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc cgatgaatgg tgccgagtgg
    29821 ctggccctgt tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa
    29881 cacataacgt tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt
    29941 attgtttcgc aaccgacggc aaatggcacc ctggttgaag cgcaagggcg tgaaatcaac
    30001 gccacgctag cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac
    30061 aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg
    30121 gagcgcatta acttccgtgg ctggccggac gcccaaatac gatgcacaga gtgctggttc
    30181 tgggggcaaa aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg
    30241 ctgaccaatg gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt
    30301 aataatccgg ggaatgaacg tacctcgctg aaaggaaaac tgcgcgggca gaaaattgat
    30361 gccgccgcag aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat
    30421 tacgatttac actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc
    30481 attcatactc aactgggtaa aggcgaaatt accgaaatca ataccggaca tgctgggcaa
    30541 ttgctgcgct tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac
    30601 acttttggcg aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc
    30661 gttatgcaca ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg
    30721 tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg ttgtcgcacc agagatttct
    30781 gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt
    30841 gccgccagta aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt
    30901 tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa
    30961 gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa
    31021 ggccaacgag ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc
    31081 cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg
    31141 taagtgaaca attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg
    31201 gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg
    31261 aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg
    31321 ttggtgtgcg tgcaatcagt ggtgaaaaaa ccggatttgc ttacgcagac caaatcagcc
    31381 tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg
    31441 gcaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc
    31501 tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgccc
    31561 gcgaagcgga caagcgcgta caagaagtga ctgccagcct cagcggcgtt tatgaattaa
    31621 ttctggttgc ggccaccgac ggcacgctgg cggcggatgt tcgtccgctg gtgcgtcttt
    31681 ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg
    31741 gtcgttttgg ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat
    31801 gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg
    31861 gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag
    31921 gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc agtatttagt gggcaggtcg
    31981 gggagctggt ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc
    32041 gtggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg ctgattgaga
    32101 acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc
    32161 cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca
    32221 cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt gagtacggta
    32281 tctatgcacc gaactttggt ggcggtcagg tggatattac atccggcaaa ttcgttttct
    32341 ccacttcaga agcgtatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcga
    32401 cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga
    32461 aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg
    32521 gccagccaac gctgaaagtt gataacctga ctgttggcgg tactgcgtaa taatttcatt
    32581 attttcagaa ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg
    32641 ttagggtgag gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta
    32701 cttctctttc ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc
    32761 cgtcagcgag ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa
    32821 cgcataaacc gggcgtggat ctgactgata acgcggcagc aggatctcca gctcaccgcg
    32881 attgatctcg ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag
    32941 ccagcgcacc agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt
    33001 cgagatccct tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag
    33061 ccatgaatgg ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct
    33121 tttcgcggcg cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc
    33181 ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg
    33241 aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt tggctgtcag
    33301 cccggcgaga acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg
    33361 ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg
    33421 gcagccctgg tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt
    33481 taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac
    33541 gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa
    33601 caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac
    33661 ataatataga taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg
    33721 ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg
    33781 cctgctctct ctttacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg
    33841 ggctgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg
    33901 tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca
    33961 acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa
    34021 gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg
    34081 gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac
    34141 gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag
    34201 gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag
    34261 ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg
    34321 gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct
    34381 cgcgaagaga tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa
    34441 gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgcgcgcca
    34501 gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca
    34561 acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact
    34621 aagctggaag gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa
    34681 gtgctgaaag ggactgttga tagtgttgcc gcaggggtca ccaacgccag cagcacgcgt
    34741 gacgacaaag ggatggcgac tatagactct aaccttgaat gggtgcgtct tgcgcaacgt
    34801 gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact
    34861 gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt
    34921 aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta
    34981 accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg
    35041 gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg
    35101 ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgt tatcgtggct
    35161 ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga
    35221 tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct
    35281 ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg
    35341 cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg
    35401 aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt
    35461 ctccgcgatc gatcaaacaa gaagtggatc gagagctgga aagtttgctg gtcgcgcaat
    35521 atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg
    35581 gcgacctggt gcgacgcacc acggcgctac aaggcatgcg cagcaacctg aatatggaat
    35641 cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc
    35701 tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg
    35761 atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc
    35821 tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt
    35881 atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca
    35941 caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagtc aaagtagagt
    36001 cagccgaacg tcatcatgcg atggttaact tctggcgaac cacactttcc tgcattctgg
    36061 gcacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg
    36121 cggtagtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta
    36181 tctacgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta
    36241 atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga tttttcctcg
    36301 gtatagaagt acagaaacgg cgactgggct cgatgggggc gctggccagc accataaata
    36361 ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag
    36421 ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gcttgtgcgg gataaatcgc
    36481 gcgacaggac cggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga
    36541 ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt
    36601 tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta
    36661 tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc
    36721 accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc
    36781 gtcggtactt tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct
    36841 ggcaagc
//
LOCUS       DAFATA010000035        36328 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFATA010000035 DAFATA010000000
VERSION     DAFATA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.035"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..431)
                     /locus_tag="LGJ20_002182"
     CDS             complement(<1..431)
                     /locus_tag="LGJ20_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBK2386237.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEG"
     gene            complement(674..2014)
                     /gene="lamB"
                     /locus_tag="LGJ20_002183"
     CDS             complement(674..2014)
                     /gene="lamB"
                     /locus_tag="LGJ20_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBK2386238.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2086..3201)
                     /gene="malK"
                     /locus_tag="LGJ20_002184"
     CDS             complement(2086..3201)
                     /gene="malK"
                     /locus_tag="LGJ20_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBK2386239.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            3566..4756
                     /gene="malE"
                     /locus_tag="LGJ20_002185"
     CDS             3566..4756
                     /gene="malE"
                     /locus_tag="LGJ20_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBK2386240.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4910..6454
                     /gene="malF"
                     /locus_tag="LGJ20_002186"
     CDS             4910..6454
                     /gene="malF"
                     /locus_tag="LGJ20_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBK2386241.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGNGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            6469..7359
                     /gene="malG"
                     /locus_tag="LGJ20_002187"
     CDS             6469..7359
                     /gene="malG"
                     /locus_tag="LGJ20_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBK2386242.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            7731..9206
                     /gene="xylE"
                     /locus_tag="LGJ20_002188"
     CDS             7731..9206
                     /gene="xylE"
                     /locus_tag="LGJ20_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBK2386243.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(9250..9660)
                     /gene="psiE"
                     /locus_tag="LGJ20_002189"
     CDS             complement(9250..9660)
                     /gene="psiE"
                     /locus_tag="LGJ20_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBK2386244.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            9875..10153
                     /locus_tag="LGJ20_002190"
     CDS             9875..10153
                     /locus_tag="LGJ20_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386245.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(10200..12296)
                     /locus_tag="LGJ20_002191"
     CDS             complement(10200..12296)
                     /locus_tag="LGJ20_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBK2386246.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(12296..13033)
                     /locus_tag="LGJ20_002192"
     CDS             complement(12296..13033)
                     /locus_tag="LGJ20_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBK2386247.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13030..13668)
                     /locus_tag="LGJ20_002193"
     CDS             complement(13030..13668)
                     /locus_tag="LGJ20_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBK2386248.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(13782..14024)
                     /locus_tag="LGJ20_002194"
     CDS             complement(13782..14024)
                     /locus_tag="LGJ20_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386249.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(14379..16028)
                     /gene="pgi"
                     /locus_tag="LGJ20_002195"
     CDS             complement(14379..16028)
                     /gene="pgi"
                     /locus_tag="LGJ20_002195"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK2386250.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            16553..17902
                     /gene="lysC"
                     /locus_tag="LGJ20_002196"
     CDS             16553..17902
                     /gene="lysC"
                     /locus_tag="LGJ20_002196"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBK2386251.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            18047..18973
                     /locus_tag="LGJ20_002197"
     CDS             18047..18973
                     /locus_tag="LGJ20_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK2386252.1"
                     /translation="MATLMEKDALLNGASQCIAFLLNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(19009..19800)
                     /locus_tag="LGJ20_002198"
     CDS             complement(19009..19800)
                     /locus_tag="LGJ20_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK2386253.1"
                     /translation="MPLNGVSAAMRERVSQQLREIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(19806..20933)
                     /locus_tag="LGJ20_002199"
     CDS             complement(19806..20933)
                     /locus_tag="LGJ20_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HBK2386254.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            21463..21735
                     /locus_tag="LGJ20_002200"
     CDS             21463..21735
                     /locus_tag="LGJ20_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBK2386255.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(21736..22608)
                     /gene="rluF"
                     /locus_tag="LGJ20_002201"
     CDS             complement(21736..22608)
                     /gene="rluF"
                     /locus_tag="LGJ20_002201"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBK2386256.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            22820..23509
                     /gene="pepE"
                     /locus_tag="LGJ20_002202"
     CDS             22820..23509
                     /gene="pepE"
                     /locus_tag="LGJ20_002202"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBK2386257.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(23600..25231)
                     /locus_tag="LGJ20_002203"
     CDS             complement(23600..25231)
                     /locus_tag="LGJ20_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBK2386258.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(25451..29134)
                     /gene="metH"
                     /locus_tag="LGJ20_002204"
     CDS             complement(25451..29134)
                     /gene="metH"
                     /locus_tag="LGJ20_002204"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK2386259.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            29334..30158
                     /gene="iclR"
                     /locus_tag="LGJ20_002205"
     CDS             29334..30158
                     /gene="iclR"
                     /locus_tag="LGJ20_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBK2386260.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            30476..32049
                     /locus_tag="LGJ20_002206"
                     /pseudo
     CDS             30476..32049
                     /locus_tag="LGJ20_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(32018..33754)
                     /gene="aceK"
                     /locus_tag="LGJ20_002207"
     CDS             complement(32018..33754)
                     /gene="aceK"
                     /locus_tag="LGJ20_002207"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBK2386261.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(34035..35339)
                     /gene="aceA"
                     /locus_tag="LGJ20_002208"
     CDS             complement(34035..35339)
                     /gene="aceA"
                     /locus_tag="LGJ20_002208"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBK2386262.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(35369..>36328)
                     /gene="aceB"
                     /locus_tag="LGJ20_002209"
     CDS             complement(35369..>36328)
                     /gene="aceB"
                     /locus_tag="LGJ20_002209"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBK2386263.1"
                     /translation="KTQSWQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQM
                     DEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLI
                     KTCHKRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADT
                     AMAVFNDILGSRKNQLEVMREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAW
                     ISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVI
                     ASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         8723 a         9551 c         9067 g         8987 t
ORIGIN      
        1 ccttccagcc gatctgcact catcacgcct ggttcctggt aggtgaaata actgctgggg
       61 aagaaggctg atggggtcat gttctgatca agaatcagca cgttcggcgc aaaaacgctg
      121 gtttgtttgt tcacttcgct ggtcagcgtc agggtcagtt cgccaatgtt tgccgggacg
      181 ctgtacgcag caaccggacc actgatgccg ggaacgttca gttgttggcc gccggtcgcc
      241 agttgggtgg tctgggtttt agattgatcg accggtgtcc aggtgagttg ttgcagcgcg
      301 gcagatggaa tggctggcgc gtcgctggtg ttttgcggta cgtagttaac atcggcaagg
      361 ctgattccag gcgcgcttgc cagtaaccct gctgataaac agagggcgat gagactttta
      421 ttcattttca ttgttttcac ctcaaaatct ggagctcagc ggtagccagg caatagcgcg
      481 ctaaacccga taatcagagg ggctttcgcc cctttcagat aatgacaacc tgtttttatg
      541 ccggatgcgg cgtaaacgcc ttatccggcc tacatttgac agccgttgta ggcctgataa
      601 gacgcgcaag cgtcgcatca ggcgttggtt gtcgaatgcg gcgtaaacgc cttatccggc
      661 ccaggttttg ctattaccac cagatttcca tctgggcacc gaaggtccac tcgtcgctgt
      721 cgccacgacc gaagctgccg ccgttgaaat cagcaggaac ggctttgccg tagttcgggt
      781 taaccttgct atcgccgttg tagtcataac cccatttctc atcccacttg gcgtaggttg
      841 cgaagacacg aatagccggg cgtgaccaga tgctgtcgcc agcctgccat tgttgtgcga
      901 gggtaatttt gtactgattg ttcttgtcgc cggtgcgctg ggattcgacg ttgtcgtagc
      961 cgatttccat cacggtgctc atgattggcg tccacttgta catcgggcga ataccaacgg
     1021 tccaccactt ggtgccgttg tcgttatccc agttgatatc ctggtacata cccacgtaca
     1081 tcatgtccca gttgtcgccc atggagatcg caccgtggtc gaggatacgc agcatgtgac
     1141 cgttgttgtt gatattgtag gcaaattttt cgttatcaaa cgcgacgcca gaaccctgcg
     1201 acagaccttt accctgcgag gtcatcgagt cagtagcgta ctgaacaaca aacttgttaa
     1261 agcccttcag gacactctga gtatgttcag cagtgaataa ccagccgtct ttcgatgcgc
     1321 catcaaccag acgatagtta tcacgcaggt tggcacgacc gtagtcgaca cccagttcta
     1381 atgtgccgcc cgggttgatt tccatctgcg ctaaacgcac atcgaaaacg tcgttcgcgg
     1441 tttcgttggt atagtcataa atattgttgc tggcaaaaga ggaagaacca ccagcttcag
     1501 aggagcgggt tgctgccaga gagagtttac cgaagccaac atcgatgttt tccagaccgg
     1561 caccaggacc agaaatatcc cagtagtaga agtcgatcat atgaacgtca tgacgttggt
     1621 agaagcgctt acctgcccag atggtggaac ctggcagcca ttcgatcagg tttttaccct
     1681 gcacgtttgc ttcacggaag gccggatcgg tagcttccca gtcattctgt tgcgcgacgg
     1741 aataggccac gttagtgtcg aaatagaagc tcttatcgcc ctctttccac acttcctgac
     1801 ccaattttaa ttcagcataa gtttcacatt cgttgccaag acggtattta ctttgagcac
     1861 cggtagtctg gaaacactgt tgttcaccgc cgctacctgt ccagccaata ccggaacgtg
     1921 catagccgtg gaaatcaaca gccattgcct gagcagacat tacgcccgct gcgacggcaa
     1981 ccgccagagg aagtttgcgc agagtaatca tcattctatc tcctgagtca ttgcttttct
     2041 tttttcacat cacctgtgac aggctttgtg tgttttgtgg ggtgcttaaa cgcccggctc
     2101 cttatgcagt cgacgacatg cagtgccatc ctcacggaac agatggcaac gctctggcgg
     2161 caggccgata gcgaatgtgg caccttcttc taccaacacc acgtcgttct ggcggtacac
     2221 caggttttga cgaatggaag ggatctggat atggatttgg gtttcgttgc cgagttgctc
     2281 gacgacctga acttcaccct caaggatgac gtcagcgata tcactcggca gtagatgttc
     2341 cgggcgaata cccagcgaca tattggctcc aacctggaca tcacggcttt caactggcag
     2401 ccagacttgc tgacgatttg gcatcggcag ctccacctgc acttgatcga ttgcggtggc
     2461 ggtcactttt accggcagga agttcatctt tggcgaaccg ataaatccgg cgacaaaacg
     2521 gtctgccgga tagtggtaca gctccagcgg tttcccaacc tgcgccacgc gaccggcgtc
     2581 cagcaccacg attttgtcgg ccagcgtcat cgcttcgacc tgatcgtggg tgacgtaaat
     2641 cattgtgcgg cccaggcgtt tatgcagacg ggagatttcg atacgcattt gcacacgcag
     2701 tgcagcatcg aggttggaga gcggttcatc gagcaaaaat acgcttggct cggccaccag
     2761 cgtacggcca atcgccacac gctgacgctg accgccagag agcgctttcg gtttgcgatc
     2821 cagcaaatgc gccagttgca gcacttccgc cacctggtta acgcgttggt taatcacctc
     2881 tttttttgcg cctgccagtt tcaggccaaa tgacatattt tctgctactg acaggtgggg
     2941 atagagcgcg taagactgaa acaccatacc aacgccgcgt tctgctggcg gagtgtcatt
     3001 catccgtttc tcaccgatga acaggtcgcc gctggtgatc gtctcaagcc cggcaatcat
     3061 gcgcagtaaa gtcgatttac cgcagccaga cggtccgaca aacaccacga attcgccttc
     3121 atggatatcg agattgatat ctttcgatac cacgacctcg ccccaggctt tcgttacatt
     3181 ttgcagctgt acgctcgcca tgcccttctc cctttgtaac aacctgtcat cgacagcaac
     3241 attcatgatg ggctgactat gcgtcatcag gagatggctt aaatcctcca ccccctggct
     3301 tttttatggg ggaggaggcg ggaggatgag aacacggctt ctgtgaacta aaccgaggtc
     3361 atgtaaggaa tttcgtgatg ttgcttgcaa aaatcgtggc gattttatgt gcgcatctcc
     3421 acattaccgc caattctgta acagagatca cacaaagcaa cggtggggcg taggggcaag
     3481 gaggatggaa agaggttgcc gtataaagaa actagagtcc gtttaggtgt tttcacgagc
     3541 acttcaccaa caaggaccat agattatgaa aataaaaaca ggtgcacgca tcctcgcatt
     3601 atccgcatta acgacgatga tgttttccgc ctcggctctc gccaaaatcg aagaaggtaa
     3661 actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa
     3721 attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa
     3781 attccctcag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cgcacgaccg
     3841 ttttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca
     3901 ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta
     3961 cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc
     4021 aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc
     4081 gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg
     4141 ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc
     4201 tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc
     4261 agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat
     4321 caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt
     4381 actgccgacc ttcaagggtc aaccatctaa accgttcgtt ggcgtgctga gcgccggtat
     4441 taacgccgcc agtccgaaca aagagctggc gaaagagttc ctcgaaaact atctgctgac
     4501 tgacgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc
     4561 ttacgaggaa gagttggcga aagatccacg tattgccgcc accatggaaa acgcccagaa
     4621 aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc
     4681 ggtgatcaac gccgccagtg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac
     4741 tcgtatcacc aagtaatgct gtgaaatgcc ggatgcggcg tgaacgcctt gtccggccta
     4801 caaaactgaa acgcatgtag gcctgataag acgcatcagt gtcgcatcag gcagttgttg
     4861 tcggataagg cgtgaaagcc ttatccgtcc tggaatgagg aagaacccca tggatgtcat
     4921 taaaaagaaa cattggtggc aaagcgacgc gctgaaatgg tcagtgctag gtctgctcgg
     4981 cctgctggtg ggttaccttg ttgttttaat gtacgcacaa ggggaatacc tgttcgccat
     5041 taccacgctg atattgagtt cagcggggct gtatattttc gccaatcgta aagcctacgc
     5101 ctggcgctat gtttacccgg gaatggctgg aatgggatta ttcgtcctct tccctctggt
     5161 ctgcaccatc gccattgcct tcaccaacta cagcagcact aaccagctga cttttgaacg
     5221 tgcgcaggaa gtgttgttag atcgctcctg gcaagcaggc aaaacctata actttggtct
     5281 ttacccggcg ggcgatgagt ggcaactggc gctcagcgac ggcgaaaccg gcaaaaatta
     5341 cctctccgac gcgtttaaat ttggcggcga gcaaaaactg caactgaaag aaacgaccgc
     5401 ccagcccgaa ggcgaacgcg cgaatctgcg cgtgattacc cagaatcgtc aggcgctgag
     5461 tgacattacc gccattctgc cggatggcaa caaagtgatg atgagctccc tgcgccagtt
     5521 ttctggcacg cagccgctct acacactcga cggtaacggc acgttgacga ataatcagag
     5581 tggcgtgaaa tatcgtccga ataaccaaat tggcttttac cagtccatta ccgccgacgg
     5641 caactggggg gatgaaaagc taagccccgg ttacaccgtg accaccggct ggaaaaactt
     5701 tacccgcgtc tttaccgacg aaggcattca gaaaccgttc ctcgccattt tcgtctggac
     5761 cgtggtgttt tcactgatca ccgtcttttt aacggtggcg gtcggcatgg ttctggcgtg
     5821 tctggtgcag tgggaagcgt tgcgtggcaa agcggtatat cgcgtcctgc tgattctgcc
     5881 ctacgcggtg ccatcattca tttcaatctt gattttcaaa gggttgttta accagagctt
     5941 cggtgaaatc aacatgatgt tgagcgcgct gtttggcgtg aagcccgcct ggttcagcga
     6001 tccgaccact gcccgaacga tgctgattat cgtcaatacc tggctgggtt atccgtacat
     6061 gatgatcctc tgcatgggct tgctgaaagc gattccggac gatttgtatg aagcctcagc
     6121 aatggatggc gcaggtccgt tccagaactt ctttaagatt acgctgccgc tgctgattaa
     6181 accgctgacg ccgctgatga tcgccagctt cgcctttaac tttaacaact tcgtgctgat
     6241 tcagctgtta accaacggcg gcccggatcg tcttggcacg accacgccag ccggttatac
     6301 cgacctgctt gttaactaca cctaccgtat cgcttttgaa ggcggcgggg gtcaggactt
     6361 cggtctggct gcagcaattg ccacgctgat cttcctgctg gtgggtgcgc tggcgatagt
     6421 gaacctgaaa gccacgcgaa tgaagtttga ttaagggaga taacaaaaat ggcaatggtc
     6481 caaccgaaat cgcaaaaagc acgtttgttt attactcacc tgctactgct actttttatc
     6541 gcggcgatta tgttcccgct gctgatggtc gtcgctatct cgctgcgtca gggaaacttc
     6601 gcgaccggta gcctgatccc ggagcaaatc tcctgggatc actggaaact ggcgttaggt
     6661 tttagcgttg aacaggctga tggtcgcatt acgccaccgc cattcccggt actgctgtgg
     6721 ctgtggaact cggtaaaggt cgccgggatt tccgcgattg gcattgtggc gctctccacc
     6781 acctgcgcct acgctttcgc ccgtatgcgc tttccaggca aagcgacgct gctgaaagga
     6841 atgctgattt tccagatgtt cccggcagta ctttcactgg tcgcgttgta tgcgttgttt
     6901 gatcgtctgg gtgagtacat tccattcatt ggcctgaata ctcacggcgg cgtaattttc
     6961 gcgtatctgg gtgggattgc gctgcatgtc tggaccatca aaggctattt cgaaaccatc
     7021 gacagttcgc tggaagaagc ggctgcgctg gatggtgcga caccgtggca ggccttccgc
     7081 cttgtcctgt tgccgctgtc agtaccgatt ctggcggtgg tattcatcct gtcgtttatc
     7141 gctgccatta ctgaagttcc ggtcgcgtcg ctgttactgc gtgacgtaaa cagctacacc
     7201 ctggccgtgg ggatgcagca atacctcaac ccgcaaaact acctgtgggg tgactttgcc
     7261 gccgctgccg tgatgtctgc attaccgatc accatcgtct tcttgctggc tcaacgctgg
     7321 ctggtcaacg gcctgacggc aggtggtgtg aaaggttaaa gatgttgttc tgccaatgtt
     7381 atgccgctgc accctcaact tacgttatcc caacttgtga ctgttattcg gcgctccacg
     7441 gagcgccttt ttttctttcg tctgcaatct gaatcgttcg ccggttaata ttcccatcat
     7501 agagcttatt atttttacgt tatttgtttt cccacttacg ataattctct ttcgtgctct
     7561 gagtcacggc aatagtattg ttttcatcaa tttttgataa ttatcacaat taagatcaca
     7621 gaaaagacat tacgtaaacg cattgtaaaa aatgataatt gccttaactg cctgacaatt
     7681 ccaacatcaa tgcactgata aaagatcaga atggtctaag gcaggtctga atgaataccc
     7741 agtataattc cagttatata ttttcgatta ccttagtcgc tacattaggt ggtttattat
     7801 ttggctacga caccgccgtt atttccggta ctgttgagtc actcaatacc gtctttgttg
     7861 ctccacaaaa cttaagtgaa tccgctgcca actccctgtt aggattttgc gtagccagtg
     7921 ctctgattgg ttgcatcatc ggcggtgccc tcggtggtta ttgcagtaac cgcttcggcc
     7981 gtcgtgattc acttaagatt gctgctgtcc tgttttttat ttctggtgta ggttctgcct
     8041 ggccagaact tggttttacc tctataaacc cggacaacac agtccctatt tatctggcag
     8101 gttatgtccc ggaatttgtt atttatcgta ttattggcgg tattggcgtt ggtttagcct
     8161 caatgctttc accaatgtat attgcggaac tggctccagc tcatattcgc ggaaaactgg
     8221 tttcatttaa ccagtttgcg attattttcg ggcaactttt agtttactgc gtaaactatt
     8281 ttattgcccg ttccggtgat gccagctggc tgaatactga cggctggcgt tatatgtttg
     8341 cctcggaatg tatccctgca ctgctgttct taatgctgct gtataccgtg ccagaaagtc
     8401 ctcgctggct gatgtcacgc ggcaagcaag aacaggcgga aagtatcctg cgcaaaatta
     8461 tgggcaacac gcttgcaact caggcagtac aggaaattaa acactccctg gatcatggcc
     8521 gcaaaaccgg tggtcgtctg ctgatgtttg gcgtgggcgt gattgtaatc ggcgttatgc
     8581 tctccatctt ccagcaattt gtcggcatca atgtggtgct gtactacgcg ccggaagtgt
     8641 tcaaaacgct gggggccagc acggatatcg cgctgttgca gaccattatt gtcggagtta
     8701 tcaacctcac ctttaccgta ctggcaatta tgacggtgga taaatttggt cgtaagccac
     8761 tgcaaattat cggcgcactc ggaatggcaa tcggtatgtt tagcctcggt accgcgtttt
     8821 acactcaggc atcgggtatt gtggcgctac tgtcgatgct gttctatgtt gccgcctttg
     8881 ccatgtcctg gggtccggta tgctgggtac tgctgtcgga aatcttcccg aatgctattc
     8941 gtggtaaagc gctggcaatc gcggtggcgg cccagtggct ggcgaactac ttcgtctcct
     9001 ggaccttccc gatgatggac aaaaactcct ggctggtggc ccatttccac aacggtttct
     9061 cctactggat ttacgggtgt atgggcgttc tggcagcact atttatgtgg aaatttgtcc
     9121 cggaaaccaa aggtaaaacc cttgaggagc tggaagccct ctgggaaccg gaaacgaaga
     9181 aaacacaaca aactgctacg ctgtaatctt cctgtccagc acgccgcgcc agttcggcgt
     9241 gctgtctttt tactcccgct tcagccgttt cgaattacac agccacagag tgatcaccag
     9301 taacaggatc gcagccgagt agatcaacac atccagtggc gatttatgat cgacgatgat
     9361 caagcgcaca atggcggtga tcccaatata gacaaagtaa cgtagcggga agtgaaaacc
     9421 ggactgaaag tatttcacaa tcagcgcgat aaattcgaaa tagagaaagt aaaccaccag
     9481 tccttctacc agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg
     9541 caccgtttct ttgccgagga agacaaccaa aatcaggccg aggcacaaca ggccaagatt
     9601 gagtacggtc tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat
     9661 agcagtacct ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct
     9721 atattttttg tgtatgtttt gttcagcttg attaagtggg catctcccat caacgtcgca
     9781 tctggcaaaa cacacagccc caaaaaatca cccattcagg tgattcccag ccaccagcaa
     9841 tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga taaaggtggt
     9901 aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc ctgcttatgc
     9961 tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct ccagcccaat
    10021 gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca ggaagacaga
    10081 atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc ctgatggctc
    10141 aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt tcccatgact
    10201 tagcggaaat ttacgctacg gtcgctggtc atatcataca actggaactt acgcccaagt
    10261 tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct gcgtgtcggg
    10321 ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa gtccccttcg
    10381 ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cgccaaccac cacgccgcta
    10441 tcaaagcgtt tggcgatctc cagcgtgccg cctttatccc ctgccagata ctgcccgacg
    10501 ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt cagatgtccg
    10561 gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca gtcgcgctgt
    10621 ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg atacaacact
    10681 tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg accgtagaag
    10741 ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac atcgttctgc
    10801 acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg cggattggtg
    10861 tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt ggtcagcaga
    10921 tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat gtaaaagttt
    10981 tccgggccac cgaccgactg gttcagcacc ggatcgatgt ggaaatcaaa gcgtgatttg
    11041 tcgatatacc agccctgctc ggtggactgc ggaaccactg gctcgacgcg tttttgcgcc
    11101 agcttcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct ggcgacatcg
    11161 gtttccgtcg tcacctgcgg catgttaaga cgattttccg taatgcggat cgttttgatc
    11221 ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc gcgcgaatca
    11281 cgatatttca cctgctcgcc ggtgacatac agcgtatcgc ctttcgcctg gatctgcgga
    11341 tcggcaagtc cggcattgta tttcaacagc gttaactgat tcgccaccac cgaatgctgc
    11401 aaaatggcat cctgaggctg cggttgatat tgcgggcggg cgttatcgtt gtaagacggg
    11461 cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt gccacgttca
    11521 tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgcaccgac gttaaactta
    11581 cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattgccttc atactccagt
    11641 ttcaggcgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag tgaggcagga
    11701 ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt atcgcgatag
    11761 caatatttat cactggctga acagagcgga tttttcacat taccgctggt gcccagatat
    11821 ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc aacaagatat
    11881 tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac ttgcggcagc
    11941 cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt atacgtttga
    12001 tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt gtagcgcagc
    12061 gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta ctgatcgtta
    12121 tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg agtttgtaat
    12181 aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata tgtttcgccg
    12241 tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt tttcattatt
    12301 ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca ggcgtctcac
    12361 tccagacgga gtccgcgagg ccaacataaa taatgctgcc gggcatcggc tctacgtgac
    12421 gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa accacccacg
    12481 cgtagctgcg atccgcaccg ctaagcaggt tctgcccgga gagatagctc gccacgtcgc
    12541 gacctggagt gaatggctgc ttgccaggac ggctgataag cccgaacagc gtgaccgtgg
    12601 acggtggtgg cccgacccac agcgtgtagt tgccctgcaa cggtgggtta ccgcgttcgg
    12661 caacgcggac gatatcagga tcaagattga ttttttgcct gcccgtcact ttcaacgcct
    12721 gaatttgctg gcgtaaggcg ttaatcgcgg cggcatcgtc ggcgctggaa tctgcagcca
    12781 gttctgccag ccgtgtcagc aacgcctgct gctgccgcaa tgctgccgcc gttgccagct
    12841 cttcgctaat caccgcacca ggccaccaac tgtttgccag ccgcggctgg cctaccagat
    12901 cgattaaata ctctgcgccc gtcagcgttt tggcctcact gctgccattg ctgaatacct
    12961 tgacggttcc cgccgcgaag accgatgacg cccccacact caaaatcaat gcgacaatag
    13021 tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga aataatccgc
    13081 gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt agcggttatg
    13141 ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccgggg tttcatcgct
    13201 gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg tggcgtagcg
    13261 tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat caacaatttg
    13321 tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt tgtcgccgcc
    13381 aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat cctgagtgac
    13441 ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg gcccgccatt
    13501 aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg tcagctgtac
    13561 gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag tcgtggctga
    13621 acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga ctcttctcca
    13681 gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata cttcaagcca
    13741 cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt actggtggtc
    13801 gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc gagcgcgaca
    13861 cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc cgaaaccgac
    13921 gttgctgccg accctgccgc ttcgcccacc tgtacaggtg cagcccacgc agaagtcgcc
    13981 gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat ttcccttcat
    14041 tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac acaactaaag
    14101 catgcgaatt acggcgcgga gaattagaga tggggtttta ggataattgg aatgaatatc
    14161 aaatataagc tacaacttat atataaataa cattaaaaat cataatgtta aaataaaagt
    14221 aaaagtaatt aatctgagta ttttccgaaa tcaatcacga ttaaaaatct attttaggat
    14281 taacctcacg gtatgatttc cgttaaatca cagacaaggg gatttatctg ataaaacaat
    14341 gccgacataa cgtcggcatt gttattaagg tttttaaatt aaccgcgcca cgctttatag
    14401 cggttaatca gaccattggt cgagctatcg tggctgctga tttctttatc atctttcagc
    14461 tctggcagaa tacggtttgc cagttgttta cccagctcca cgccccactg gtcgaaggtg
    14521 aagatgttca ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca
    14581 cccaggctga acggagtgat ttcacgcagc aggatggagt tggtcgggcg gttaccttcg
    14641 aacactttga acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat
    14701 tcctgctcaa ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag
    14761 ttagacagca gtttctggtg atgatcagag agcgggttat gggtgatagc cggagcgatg
    14821 aaatcgcacg gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg
    14881 ttagtgcctg gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta
    14941 cggtcaacat acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg
    15001 tgcatatact ggtcatacgg cagaatcgcc tcagtttccg caccaaagaa attgttgtac
    15061 cagatgccaa tcagcgccag cagtacaggc aggtttttct cggcaggcgt ggtggagaaa
    15121 tgcttgtcca tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag
    15181 agaacaatcg acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag
    15241 aactcgaaca tgttggcagt atcaatacca aactcgccaa cggctttggc attggttgag
    15301 agcgccgcaa agtgtttcgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca
    15361 cgcgcgctat gggcgttggt catggtttcc tgagtggtga aggttttaga tgctaccagg
    15421 aacagcgtgg tttccgggtt tacttttttc agcacttccg cgatgtgagt cccatcgacg
    15481 ttagaaacaa agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg
    15541 tacgggccga ggtcagaacc gccgatcccg atgttcacta cgtcagtgat tgctttgccg
    15601 gtataaccct tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc
    15661 accgcgttga cttccggcat tacgtctttg ccatcaacca aaatcggggt attgctacgg
    15721 ttacgcagcg ctacgtgcag cacagcgcgg ttttcagtgc ggttgatctt ctcgccagag
    15781 aacatcgact taatcgcgcc cgccagatcg cactctttcg ccagatcttg taatttcgcc
    15841 agcgtctctt cagtgatgcg gtttttggag taatccacca gcatctgatc gccgaaggtt
    15901 gcggagaact tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct
    15961 ttcatttcat cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg
    16021 tttttcatta gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc
    16081 agtcacagaa aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta
    16141 gcgcagtgaa gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca
    16201 tgaccccgtc gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct
    16261 agcgcaggcc agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga
    16321 taacacctga gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc
    16381 tacaggggct gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag
    16441 tggtggagca cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc
    16501 cgctcttccc ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga
    16561 aattgttgtc tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag
    16621 cgctgatatt gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg
    16681 tatcactaat ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa
    16741 actcgacgct attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt
    16801 tatccgtgaa gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc
    16861 gctggcaacg tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac
    16921 cctgctgttt gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg
    16981 taaagtgatg cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc
    17041 ggaactggcc gcgctgcagc tgctcccacg cctcaatgac ggcttagtga tcacccaggg
    17101 atttatcggt agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta
    17161 tacggcagcc ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt
    17221 cccgggcatc tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat
    17281 cgcgtttgcc gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac
    17341 gttgctaccc gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg
    17401 cgcaggtggt acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc
    17461 gcttcgtcgc aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg
    17521 cttcctcgcg gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac
    17581 cacgtcagaa gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga
    17641 tacgttgctg acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga
    17701 agaaggtctg gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg
    17761 caaagaggta ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc
    17821 cagccataac ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcagaagct
    17881 gcattttaat ttgtttgagt aatatttccc tactggcgta taacgctcac cgattgttat
    17941 acgccatcct cactgaaaac atagattaaa taatatctga aaagcaaaat cactcctgta
    18001 gaacagtatt ataatcaccg aaaaatatca tttaacagga gtgttaatgg ctactttaat
    18061 ggaaaaagat gcacttttaa acggagccag tcagtgcatc gcgtttttat taaatatcat
    18121 tgataattgc tcagtttcgt cgcatcagga ttcaggcgat gctctcaagc gattagtcag
    18181 ctatcgcgat tatctttatt ctacccccgc tgaacttgtt gattttacac aaggtaagat
    18241 tctccttcag caggtaagaa ctcaatatca gcatgaattc aacaatacta cgcattcaga
    18301 aaataaagca tcttttgatt ctatttggca gcgtctgaca aatcatgaag tcacaccgca
    18361 acagcatccc attggttttg ttttaggtgg gcaacccgga gcaggtaaat catctctgat
    18421 tgaattagcc aaaagagaga caaaagataa tataatgatt attaacggtg atgattttcg
    18481 atttctacac cctgatttta attatatcta ccaaaattat ggggatgatt ttgttaccca
    18541 tacagcgaaa ttttccggcg aaacggtcga acgtgcaatt gagagagcga tagtcagtaa
    18601 actcaacatt gttgtcgaag gaacatttcg taatgccgcg acgccgttac aaacattaaa
    18661 aaaactcaaa gatgcaggct accaaacaga agtcatgata aaaacaacct cagcggcatt
    18721 aagctgggaa agcaccaatg aacggtatag taaggacaaa gaggcgggaa atattgcccg
    18781 aaaagtggat aaaaaccacc atgatatcgt caccggtttg ctggcagaaa atgcccgcaa
    18841 ggtgtttgct tcgaacctgt cagataagtt tgcggtgtat tcgcgtgaga agatgatatt
    18901 tagttcacaa gcggcaacga atgacgatat tgcaacactt atacagaatg aaatcagcgg
    18961 gaatacacaa taataataag agctgatggc gtgaaataac gccgggcatc acgcccggcg
    19021 cattaccgtc tggtacatta agctatccag ttccttaacg tccccttctc tgctatccgg
    19081 taacagcggt ggaatttccc cacgcgccag ttccgcacga ataaacgcat gtaacaatgg
    19141 acggcgcgga ccatattccg cttctcctgc gcgctgttta cgttccagta attcgtctat
    19201 ttctgcacgt aacggcgcat caagttcgct tcccgccagt agctcagcaa agcgcattgg
    19261 cggtacgcct tttcccgctt caacccaacg cacggccagc agcggacgca gcacgtaaaa
    19321 gtattttttc agccgcactt cgtcgccttg taaataaccg cgaaagtttt tccgcgccat
    19381 cgaataataa tgccaacgcg cacgcagcgg agaaaaccac ttcggcacca ttgccttaag
    19441 cgccgttatc gtttcttcat cttgttgata gaccacagga gaatccagcc attctatcag
    19501 cgtcggattc gcacctttca ataatcccag cgccttgcgc cactcccagc ctgaaacatc
    19561 taactcgtcg tcaataggca actcaataac atcgcgcggc gcctctactc gcagatacca
    19621 ttccagcggg tgaacataca aaaaccgtac atcgtagtcg ctgtcgggcg aggcaaatcc
    19681 ccagccgcgg ctccctgatt cacaggcgta cagtactttc acatcatacc gccgttcaat
    19741 ttctcttaac tgctgcgata ccctttctcg catcgcagca ctgactccat ttaatggcat
    19801 gttactcacc ctttgatatt aaccaaccca tgcagaacct gatccagacc gccaaataca
    19861 gagatgttat cgatccgttc agccactctc tccagcgttt ccagttcttt taagcgtaac
    19921 gctaccgggt tgttttccat gactttagcg gtattgagta acgaacgcgt cgctgccgtt
    19981 tcttcacgtc ggcgaatcac attggcctgc gcggatttct ccgcctcaac cagctgcgca
    20041 aggatatttt tcatatcgcc aggcaggacg atatctttca cccctaacga ggcaatctcc
    20101 ataccaaatg gcaacatgcg cgatttcacc tgttcgctga caacgtcatc aatgacctgt
    20161 ttatcttcca gcaattcatc caacgtacgc gtacctaccg cttcacgcag ggcaaattgc
    20221 aattcccgat acaaatgatc aatcggcttc gttaactgac taaatgccag caacacgtcg
    20281 ctgtagcgcc agttagcggc gaggttaatc cgcagattga ccttatcttt cgttaagatt
    20341 tcttgcccgc tgacttccag tacctgcaag cgggtgtcaa ccacttcagc gtcaaccaga
    20401 tggttaattt tccagtatgc ggtcagcccc ggcggtaaca gcgcctgcgt ttcaccatcg
    20461 attttcagca cgcctacatg ccaggccgga acctgcaccg tgagtatcgc atcgcggcct
    20521 ttaactgccc ctttgcgccg tggttgcaat accgcattca tcacctccgt ttgtacctgg
    20581 acttgctgcg tattcatccg caccagtgtc aaatcatcct ctacacgcca gtacaaacgg
    20641 cgcgttgatg gcggcaggat ctcctgcaaa ataccgtcca tatacaacgc cccggcttca
    20701 atctccgaca agtctgccac caggcaatat ttctccaccc aatcaggctg aaagcggcgc
    20761 aaataatccg ccagcacgtc gggaacttcg ctgccatcga gcgtaatcag caagacttct
    20821 ggcgtattta accagggtaa aagatgctcg cctgcatgta gcactttgta gtaatcgccg
    20881 tttctggaga gtaatgccag ctgatctttt cttacgctta tttttttaat cattatctat
    20941 tccttgctta acagccgtcg cggaagcaga cacgaagcga caggggaatt cccttttgcc
    21001 tgttacccgg catctttatc ggcgacgtta tcggggatcg ctgactgcgc aatccctcac
    21061 tttttattta ataagttaaa accagtattc atttcaggaa tgaatgtggg aatcgaaccc
    21121 ataattggcg tcaaaggctg acggaacacg cccggaaaac agcctggccg ttcttggtgt
    21181 gccgacgggg tttgaccccc tttgtaattc agtttcaacg cgtacgagat attgcgagaa
    21241 gcgtgccaga attgagaaaa aattattaac caaatgaaaa ataaagattt aataaattat
    21301 ttttgtttgt agagggaaaa cttttcgcta aatttgtatc tgtatttatc gtcaattatc
    21361 aagaaagata aatatttaag aagagaacaa aggaatatta tcagttcagc tcgcaaacga
    21421 attcccgtac actgaactct ctggctcact gagggcacca tcatggcact cccccgtatt
    21481 acccaaaaag agatgactga acgcgagcag cgtgaactta aaacactgct ggatcgggcg
    21541 cggattgcgc atggtcgcgt actgaccaac tccgaaacca acagcattaa gaaagagtac
    21601 atcgataaat taatggtcga gcgtgaggct gaagcgaaaa aagcccgcca gttgaagaaa
    21661 aagcaggctt ataaaccgga tcctgaagcg tcatttagct ggtcagcaaa tacgtcgacg
    21721 cgcggcaggc gctgattagc gccccttctt tttacgcccc ggagaggtaa aacgcttacc
    21781 gttggacgcc gggcgaccgc ctttttccgc cgttttttcc atcttaacga ctggacgttt
    21841 gatgcccgct gttttcggtt tggccttcac tttaggtttt gcctcggaag aggaattttc
    21901 gatgagctta aagaggtcga ttaactcatc atcagttaaa tcgcgccact cacccagcgg
    21961 aatgccgctt aaactgacgt tcataatgcg cgtacgttcc agctttttca cttcatagcc
    22021 gaaatgctcg cacatgcgcc ggatctgacg gttcagcccc tgcaccaggg taatgcggaa
    22081 gacaaacggc gcttcttttt tgactttaca ctttttagtc actgtcccga ggattggcac
    22141 ccccgccccc ataccacgaa taaattcatc ggtaatcggt ttatcgaccg taaccagata
    22201 ctctttctca tgatcgttac ccgcacggag gatcttattc accagatcgc cgtgattggt
    22261 gaggaaaatc agcccctggg agtctttatc caggcggcca atcgggaaca cgcgtttgct
    22321 gtggttaacg aaatcgacaa tgttatcgcg ctcgccatct tcggtggtgc tgacaatacc
    22381 gacgggcttg ttcagggcga taagtaccaa atcttcggct tcccgaggct caatcaactg
    22441 accgtttact ttcacaacgt cgccgggttt cacctgatcg ccaatggtgg ctcgcttgcc
    22501 attaaggaac acattgcctt gctcgatata gcgatccgct tcgcggcgtg agcaaattcc
    22561 gctttcgctg atgtatttat ttaaacggac tgatgagtcg ggcagcataa attctcctgt
    22621 aaaaaacgga atataccgta cctttgggtt gataaaaaat agtcatgagc gaactatttg
    22681 ttatttccat tggcttgaat tcgttacctg ctcaaatcac tttccagaag caatgtgcga
    22741 catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct cccgtgattt
    22801 cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa
    22861 agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc gccgctcagc
    22921 ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag cgaaaacggc
    22981 tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg tcgatcccgt
    23041 tgccgcgatt gaaaatgctg agatcgtgat tgtcggcggc gggaatactt tccagttgct
    23101 gaaacagtgc cgcgagcgcg ggctgctggc accaattact gacgtggtta aacgtggcgc
    23161 tctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa
    23221 cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc cgttgcaaat
    23281 caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgagcagcg
    23341 tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctac cggaaggtaa
    23401 ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca cttatgtgtt
    23461 taaggctggt gaagaagcgg tgccgctaga agctggtcac cgtttttaag tgaattgccg
    23521 gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc gtcttatcag
    23581 gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc ggctgttcca
    23641 gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct cccagtaagc
    23701 ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg cgatcgacgt
    23761 gggcgtggga atagcggcga ttaagaatgc gaaagcgatg cttactacgg cgcaaacgac
    23821 gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa ttactgagca
    23881 attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc cgccgtgctg
    23941 ccagtgattt gtcggcaatt tcactcccca tgcgctcgac gatatcggag gcctgttcaa
    24001 ggttgagcga catttcgatg atctctgccc agcggcgcga ctcttcctcc gccagctctt
    24061 cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg atatcatctg
    24121 ccagcttacg cagctctttc tcctgccgtg gctcaccgtg catcacttta ttcaaccctt
    24181 ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg ttcgccagag
    24241 caagcgtggg cgtatccagc gcgctgacat ccagatgttt aggccgtagc tgggtatcca
    24301 gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca acaaatggca
    24361 gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc agttccgctt
    24421 ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc aggataatca
    24481 ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct gcggcattgg
    24541 cggcactgtt gttgagcatc gccagcagac cggaaccgag gttagcacca atcaccagac
    24601 agagcgccac ggggaaggag ataatgcctg cggcggtcag agtcgcggtc agcagtacag
    24661 cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca tccagcagaa
    24721 tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg atcggcgtta
    24781 cggcctgcac aatcaactcc agcgctagca aaatcagccc aagaccaata ccgacgcggc
    24841 ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg ataaaaataa
    24901 gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc gccgtcccga
    24961 catctgcacc cagcacaatg accagagccg gtgcgagggc taccagatcc tgagcgacaa
    25021 acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta acgccgatcc
    25081 ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta cgcaaacgcg
    25141 cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc agcagggcga
    25201 cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat cgttatatgt
    25261 tcctgaagca attgaatgac caaaggaccg gaaacacgtt gactggcatc tttactgtgc
    25321 cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta taagtgtttc
    25381 atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg aaaagtgaca
    25441 gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc tcaacttcgg
    25501 taacgctcat acctttacgg cgggcatagt cttcaacctg atcgcgctga atttgcgcta
    25561 ccgcgtaata cttgctgtcc gggtggctga agtaccaacc cgaaaccgat gcaccaggcc
    25621 acatggcgaa agattctgtg agtttcatgc cagtgtgttt ttccacttcc agcagctccc
    25681 agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc ggacggatgc
    25741 cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc gcatagcccc
    25801 agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct aaacggtcgg
    25861 caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag gcatcagcca
    25921 gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct gctttaccag
    25981 aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt ttttcggtct
    26041 gttgacgcag atggtggctg acgttgatca catgagtgcg cgtttcgtca cggtagattt
    26101 caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc ggattcagcg
    26161 ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct
    26221 caacgcctac cacttcatct tccagaatgc gcggatactt cccggccagc gaccaggtca
    26281 taaagaacgg tgtccagtcg atgtaattgc gcagcgtttc gatgctggct tcgacttcct
    26341 gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaaa gcgaaatcgt
    26401 tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgcgg cttcttgcgc ccgtgctgaa
    26461 tacgtacggt ttcgtactcc ttgcgggtac gagcgacaaa atcatcacgc tgggtatcgg
    26521 aaagcagcgc cgccaccaca ccaacggtac gcgatgcgtt ctgcacatac accgtcgggc
    26581 cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc gcgccgccaa
    26641 tcaatagcgg aatagtgaag ccctgacgct ccatctcttt cgccacgtta accatctcgt
    26701 ccagcgacgg cgtgataagc cccgaaagtc caatcagatc agcattcact tctttagcgg
    26761 tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg tagttgttac
    26821 attgcagcac cacaccaacg atatttttac cgatgtcgtg gacgtcgccc ttcacggtgg
    26881 cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata aacggttcga
    26941 gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc aggaacattt
    27001 tcccttcgcc aaacagatcg ccgaccacgt tcataccgtc catcagcggg ccttcaatca
    27061 cttcaatcgg gcgcgtagcc tgctggcggg cttcttcggt atcctgctcg ataaactcgg
    27121 taatgccttt aaccagcgag tattccagac gtttattcac ttcccacgag cgccactccg
    27181 cctgctgggc gttagcggtg tcgtcggttt tgctgccgcg atatttctcg gcaagctcca
    27241 gtaaacgctc agtgccatcg tcgcgacggt taagaatcac atcttccacc gcgtcgcgca
    27301 gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc cccatatcca
    27361 tgccattgcg aatagcgtag tagaggaaca ccgcgtgaat ggcttcgcgc accggatcgt
    27421 tgccacggaa tgagaaagaa acgttagata caccgccgga aatcagcgcg tgcggcagtt
    27481 cgcgtttgat atcttcacac gcgccgataa agtcctgcgc gtagttgttg tgctcttcaa
    27541 tgccggttgc gaccgcgaag atgttcgggt cgaagatgat atcttctggc gggaagccaa
    27601 cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt gcgcgggtat
    27661 cggcctgtcc ctgttcgtca aaggccatta ccaccactgc cgcaccgtaa cgacgcaaga
    27721 gcttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag ttaacaatgc
    27781 ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag gagtcgatca
    27841 taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa cgcaccatcg
    27901 ccgcttcggc gtcgagcatc ccttcatcca tgttgatatc gataatctgc gcaccgtttt
    27961 ccacctgctg gcgggccaca tccagtgcct cgctgtattt ctcttctttg atcaggcgct
    28021 tgaacttagc ggaaccggtg acgttggtgc gttcacccac gttcacaaac aggctatctt
    28081 cgccaatgtt cagcggctcc aggccggaca aacggcaggc tacggggatt tccggcagtt
    28141 tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgt ggcgtggtgc
    28201 cacagcagcc gccgacgata ttgagaaaac ccgcttgcgc ccattcacgt atctgttttg
    28261 ccatcgtgtc ggcgtcgaga tcgtactcac caaaggcgtt gggtagcccg gcgttcgggt
    28321 gcgcggtgac gtagcattcc gcaatccgtg acagctcctg cacgtactgg cgcagttcat
    28381 ctggccccag cgcacagttc aggccaaagg tcagagcttc ggcgtggcgc aatgagttgt
    28441 aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg atggtgccgg
    28501 agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc gcaaagaccg
    28561 ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc gcaccacctt
    28621 ccaccagcgc tttggtggac tctcgataag ccgccaccag cccgtcaaaa gtgatattac
    28681 gaaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt gggccgagaa
    28741 caccggcaac gtagcgcggt ttctccgggg tgcgcgcggt ccactcatca gcacaggcgc
    28801 gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc tggtaatccg
    28861 ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg cccgcttcaa
    28921 agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc aggtcgttgt
    28981 tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg gcttcgttca
    29041 gtcgatagct ctggatcatg gtgcccatac cgccgtccag caccagaata cgttcattta
    29101 actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg ctcaacccag
    29161 atttaataaa aattcaacaa accatactgg cataaacgca tctgtggtaa aagcgaccac
    29221 cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa ctattgcatt
    29281 agctaacaat aaaaatgaaa atgatttcca cgatacagaa aaaggagact gtcatggtcg
    29341 cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca gcgactggac
    29401 aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa tccaatggca
    29461 gtgtggcact cacggaactg gcgcaacaag ccgggttacc caattccacg acccaccgcc
    29521 tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt tggcgaactg ggacactggg
    29581 caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt aatttgttag
    29641 caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg gtcaatatgg
    29701 cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag tgtacccacc
    29761 tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc ggtgcgggta
    29821 aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt
    29881 tacatgccta tacccacgca acgctggtgt ctcctgtgca tttaaaagaa gatctcgccc
    29941 aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg ctacgttgcc
    30001 ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttca atttccggac
    30061 cgatttcacg tattaccgat gaccgcgtga ccgagtttgg cgcaatggtg attaaagcgg
    30121 cgaaggaagt gacgctggcg tacggtggaa tgcgctgact tttttctggc gggcagaggc
    30181 aatattctgc ccgtcatatc tgagtggcaa tagaataagg gtgtctgtta atcgcattga
    30241 cgccaaaata acttattgtc atacacttcg cacactttta ttaactcagc attattttta
    30301 aacatcaaac cacttaatta ttaacaaaat cgcaagatct cattacgccg aaacttttac
    30361 ggtttcatta tgagattgag cgatttatga tatttcttta gttgtaatca tattaaattt
    30421 tttcactgtt ctgataatta aaattcaaga catcaataaa caatgagata tttaaatgat
    30481 tactcgtatc cctcgtagtt ctttctctgc aaatattaat aatacagccc agacaaatga
    30541 acaccaaacc ctgagtgaat tgttttataa agaactcgag gataaatttt ctggcaagga
    30601 gctggcgacg cctctattaa aaagcttctc agagaactgt agacaaaatg gtcgtcatat
    30661 ctttagcaac aaggattttg tcattaaatt ttccacgtcc gtcttacaag ctgataagaa
    30721 agaaattacg ataattaata aaaacgaaaa cacgacactt actcaaacca ttgccccaat
    30781 atttgaagaa tacctaatgg aaattttacc tcaacgctca gacactcttg ataaacaaga
    30841 attaaaccta aaatcagata gaaaagaaaa agaattccca agaattaaac ttaatggtca
    30901 atgttatttt ccggggcgac cccaaaaccg tatagtatgc cgacacattg ctgcacaata
    30961 tattaataat atttatcaga atgttgatta caaaccccat caagatgatt actcttcagc
    31021 tgaaaaattt ctcacgcact tcaacaaaaa atgcaaaaac cagactttgg cgttggtttc
    31081 cagccgtcct gaggggcgtt acgttgctgc ctgtggtgat ttcgggctag ttatgaaggc
    31141 atatttcgac aagatggaat caaatggcat cagtgttatg gcagccatat tattggtgga
    31201 taaccatgct ttgacggcgc ggctaagaat aaagaacaca actgaaggat gtacccatta
    31261 cgtggtttcg gtttatgatc ctaatgtcac taacgataaa ataagaatta tgagcgaaag
    31321 caaagaggat attaaacact attctctgat ggattttatg aatgtagatt atagcctcct
    31381 gaaatggtca aatgatcata ttattaacca atctgttgca ataattccag cacttccgaa
    31441 agaacagcta ttgatgttaa aaggatctgt ggatgaaata acccctccat tatcacctgc
    31501 aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac tgatgattca
    31561 actccagaaa atgccagaac tacatagaac agaaatgttg actgcctata atagtggaca
    31621 tatgaacgtt attaatacta tttttaacgc attacccact ctttttaata cgtttaaatt
    31681 cgataaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata tcccggtttg
    31741 ttttcagcga tacagcataa acaacaaaac gttgtagaga cggtttatct tgctttatct
    31801 gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg gcaacacaaa
    31861 gccccacaaa aatactctgc ctttgagttg gcttttgaat tgggtcaccg ggttattgct
    31921 gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac ggataaccct
    31981 cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc tccccatacc
    32041 gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc ccttcacgta
    32101 tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc atctcttcaa
    32161 acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc gggaaaacat
    32221 cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga tagcgcggcg
    32281 gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa tcataaaaaa
    32341 ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca ggaaaaatgt
    32401 tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg cgcaactgct
    32461 gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctcg atatagagat
    32521 ggcgaattac aatctgctca ccaagatcgg tgattttttc cgctgcttcc tggagcagta
    32581 attccattaa tgccggggaa atatgccgct tctccagcac aaagttttca aactcctggg
    32641 tgtccgccat tcggcccaca cggtcgtgct ctttcaccag ttgatagcag gcacgaacgt
    32701 gagcggcaga catctctttc tgcggcgcga acttgtcttt gatgactttg aatacccgat
    32761 caaagcccgg cagcgtaaat accaacatca ccattccacg aatacccggc gcttcaatga
    32821 actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg gttttggcgt
    32881 gcttctggca gccgatagcc atatacaatt cagcagtggt tttacctggc agaatttccc
    32941 gtagccactc gaccagcgct gcgggcagcg gtgcataaac cataaaataa gaacgcgcaa
    33001 agccaaaaac aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata aataactcgc
    33061 cgtcgtccgt ctggtggatc ggcagtaaga agggcaacgt gccggaaggc gtgatcagtt
    33121 tgcccaccag ccaggcggct ttattgcggt aaaacagttc gttcgccacc tgtaaatgac
    33181 tttccgcgag gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg taatgaatgt
    33241 cacggctttt attctgccag cgcaggcgca gcggcaggtc gctgataacg cgcatcagta
    33301 gagattccca gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga atggtacgaa
    33361 agcggcgctc tggctgagag ctaaagataa aaagccgctc gggagtaagc gagcggtggt
    33421 caaataaccg acagtacacg gagttaaaaa agctctccgc aatctcgaag cgcgggtaat
    33481 ccggcaacag ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg tccgtacttt
    33541 ggccgttagt aatgcagcgc agttgctcca cgaccagacc aacgtgatga tcgtaaagat
    33601 ggatacggtt tttcatcgcc tgctggacag catgccagtc ggcctgttcg aaacgctgct
    33661 gcgccccgga agtcacttcg aggaatcgac catactgagc gtcgaagcct tgcaaaatgg
    33721 tttgagcaat cagtaattcc aggccacgcg gcatttagcg ccctcatcag gagcagagaa
    33781 ttgccggatg cggcgtaaac gccttatcca gcctacgttc ggcaacggtt gtaggcatga
    33841 taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtaaatgc
    33901 cttatccggc ctacgttcgg caacggctgt aggcatgata agacgcgcaa gcgtcgcatc
    33961 aggcatcgtg caccgattgc cggatgcggc gtgaacgcct tatccggcct acagtcagca
    34021 acggttgttg ttgcttagaa ctgcgattct tcagtggagc cggtcagcgc ggtgactgaa
    34081 gacgtgccgc cctgaataat agtcgtcact ttatcgaagt aacctgtacc cacttcctgc
    34141 tggtgagata cgaaggtata gccatctttc gcggcggcaa attccggctg ctgcactttc
    34201 tcaacgtagt gcttcatacc ctcgccctgg gcataggcgt ttgccaggtc aaacatgttg
    34261 aaccacatgc tgtggatacc tgccagggtg atgaactgga acttgtagcc catatccgac
    34321 agctgctgct ggaagctggc aatagttttg tcgtcgaggt ttttctgcca gttgaacgac
    34381 ggcgagcagt tataagccag cagtttgccc ggatatttcg cgtggatagc ttgtgcaaag
    34441 cgacgcgcca gttccagatc cggcgtggag gtttcacacc agaccaggtc agcatatggc
    34501 gcatacgcca ggccacggct gatcgcttgc tcaatgcccg catgagtacg gaagaagcct
    34561 tcactggtac gctcgccagt aataaattca ctgtcatacg ggtcgcaatc ggaggtaatc
    34621 agatccgccg catcggcatc ggtacgagca accagcaggg ttggaacgcc cgtcacgtca
    34681 gctgcaagac gcgccgcgac cagtttctga atagcttcct gagttggcac caaaactttg
    34741 ccgcccatgt gaccgcattt cttcactgac gccagctgat cttcgaagtg aactgccgct
    34801 gcaccggctt caatcatcgc tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg
    34861 gcttccgcat cggcaacgat cggcaggaag taatcgacat agcgcggatc gcccggctca
    34921 atgcctgcgg accattggat ctgatcggca cgacggaagg tgttgttgat ccgctccacc
    34981 acagctggca ccgagtttgc cggatagagc gactgatccg gatacatgct ggccgccagg
    35041 ttagcgtccg ccgctacctg ccatcccgac agatagactg cttcaatacc cgctttcgcc
    35101 tgttgcagcg cctgaccgcc agtcagtgca ccgaggctgt tgatgtagcc ttttttcgac
    35161 tcgccatgca gcagacgcca cattttcgcc gcgcccagtt gcgccagggt gcattcagga
    35221 ttgactgaac cgcgtaattt caccacatct tccgcgctgt atgggcgagt aatgccttcc
    35281 caacgcggtt gagtccactc tttctgtaat tcttcaattt gttgtgtacg ggttttcatg
    35341 tgcagatgct ccatagttat gtggtggttt acgctaacag gcggtagcct ggcagggtca
    35401 ggaaatcaat taactcatcg gaagtggtga tctgttccat caagcgtgcg gcatcgtcaa
    35461 aacgcccctg ggagaaacgt tcttcgccca gttcgctggc aatgactttc atctcttcac
    35521 ccagcatctg gcggaacaag gctttggtca ccggtttgcc attgctcaac gttttttgat
    35581 gatggatcca ctgccagatt gaggtacggg aaatttcagc cgtcgccgca tcttccatca
    35641 ggccataaat cggcacgcag ccgttgccgg agatccatgc ttcgatgtac tgcactgcca
    35701 cgcgaatgtt ggcgcgcata ccttcttcgg tgcgttcacc atcacaaggt gccagcagct
    35761 gatcggcagt aatcggcacg tcttgttcgc gcatcacttc aagctgattt ttacgggagc
    35821 cgagaatgtc gttgaatacc gccattgccg tatccgcaag gcctgggtga gcaatccatg
    35881 tgccatcgtg accgttattg gcttccagcg ctttatccgc ttttactttg ttgagcacct
    35941 ggttattgcg ctcttcatct ttgctcggaa taaacgctgc catgccgccc atcgcaaaag
    36001 caccgcgttt atggcaggtt ttaatcaaca ggcgtgagta agcattcagg aatggtttat
    36061 ccatcgtcac tgcctgtctg tctggcagga cgcgatcggg atagtttttc aacgttttga
    36121 tatagctgaa gatgtaatcc caacgaccgc agttcagacc aacaatatgg tcacgcagcg
    36181 cgtgaaggat ttcatccatc tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct
    36241 tgatggtgcc gcgcggcaga ttaaagcgat cttctgcata gctgaagact tcgctccacc
    36301 aggccgcttc ctgccaggac tgggtttt
//
LOCUS       DAFATA010000036        35896 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFATA010000036 DAFATA010000000
VERSION     DAFATA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.036"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..206)
                     /locus_tag="LGJ20_002210"
     CDS             complement(<1..206)
                     /locus_tag="LGJ20_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK2386264.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKG"
     gene            complement(203..1447)
                     /gene="ftsW"
                     /locus_tag="LGJ20_002211"
     CDS             complement(203..1447)
                     /gene="ftsW"
                     /locus_tag="LGJ20_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBK2386265.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(1447..2763)
                     /gene="murD"
                     /locus_tag="LGJ20_002212"
     CDS             complement(1447..2763)
                     /gene="murD"
                     /locus_tag="LGJ20_002212"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK2386266.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGLTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(2766..3848)
                     /gene="mraY"
                     /locus_tag="LGJ20_002213"
     CDS             complement(2766..3848)
                     /gene="mraY"
                     /locus_tag="LGJ20_002213"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK2386267.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(3842..5200)
                     /gene="murF"
                     /locus_tag="LGJ20_002214"
     CDS             complement(3842..5200)
                     /gene="murF"
                     /locus_tag="LGJ20_002214"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK2386268.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(5197..6684)
                     /gene="murE"
                     /locus_tag="LGJ20_002215"
     CDS             complement(5197..6684)
                     /gene="murE"
                     /locus_tag="LGJ20_002215"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBK2386269.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(6671..8437)
                     /gene="ftsI"
                     /locus_tag="LGJ20_002216"
     CDS             complement(6671..8437)
                     /gene="ftsI"
                     /locus_tag="LGJ20_002216"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBK2386270.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(8453..8818)
                     /gene="ftsL"
                     /locus_tag="LGJ20_002217"
     CDS             complement(8453..8818)
                     /gene="ftsL"
                     /locus_tag="LGJ20_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK2386271.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(8815..9756)
                     /gene="rsmH"
                     /locus_tag="LGJ20_002218"
     CDS             complement(8815..9756)
                     /gene="rsmH"
                     /locus_tag="LGJ20_002218"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK2386272.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPITEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(9758..10216)
                     /gene="mraZ"
                     /locus_tag="LGJ20_002219"
     CDS             complement(9758..10216)
                     /gene="mraZ"
                     /locus_tag="LGJ20_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK2386273.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(10818..11822)
                     /gene="cra"
                     /locus_tag="LGJ20_002220"
     CDS             complement(10818..11822)
                     /gene="cra"
                     /locus_tag="LGJ20_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBK2386274.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(11904..11990)
                     /locus_tag="LGJ20_002221"
     CDS             complement(11904..11990)
                     /locus_tag="LGJ20_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBK2386275.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(12002..12493)
                     /gene="ilvN"
                     /locus_tag="LGJ20_002222"
     CDS             complement(12002..12493)
                     /gene="ilvN"
                     /locus_tag="LGJ20_002222"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBK2386276.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(12496..14220)
                     /gene="ilvI"
                     /locus_tag="LGJ20_002223"
     CDS             complement(12496..14220)
                     /gene="ilvI"
                     /locus_tag="LGJ20_002223"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBK2386277.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            14339..14383
                     /locus_tag="LGJ20_002224"
     CDS             14339..14383
                     /locus_tag="LGJ20_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386278.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(14538..15482)
                     /gene="leuO"
                     /locus_tag="LGJ20_002225"
     CDS             complement(14538..15482)
                     /gene="leuO"
                     /locus_tag="LGJ20_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBK2386279.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            16142..16228
                     /gene="leuL"
                     /locus_tag="LGJ20_002226"
     CDS             16142..16228
                     /gene="leuL"
                     /locus_tag="LGJ20_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBK2386280.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            16321..17892
                     /gene="leuA"
                     /locus_tag="LGJ20_002227"
     CDS             16321..17892
                     /gene="leuA"
                     /locus_tag="LGJ20_002227"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBK2386281.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            17892..18983
                     /gene="leuB"
                     /locus_tag="LGJ20_002228"
     CDS             17892..18983
                     /gene="leuB"
                     /locus_tag="LGJ20_002228"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBK2386282.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            18986..20386
                     /gene="leuC"
                     /locus_tag="LGJ20_002229"
     CDS             18986..20386
                     /gene="leuC"
                     /locus_tag="LGJ20_002229"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBK2386283.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            20397..21002
                     /gene="leuD"
                     /locus_tag="LGJ20_002230"
     CDS             20397..21002
                     /gene="leuD"
                     /locus_tag="LGJ20_002230"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBK2386284.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(21051..22229)
                     /gene="setA"
                     /locus_tag="LGJ20_002231"
     CDS             complement(21051..22229)
                     /gene="setA"
                     /locus_tag="LGJ20_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBK2386285.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(22331..22462)
                     /gene="sgrT"
                     /locus_tag="LGJ20_002232"
     CDS             complement(22331..22462)
                     /gene="sgrT"
                     /locus_tag="LGJ20_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBK2386286.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            22551..24206
                     /gene="sgrR"
                     /locus_tag="LGJ20_002233"
     CDS             22551..24206
                     /gene="sgrR"
                     /locus_tag="LGJ20_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBK2386287.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      24240..24339
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24370..25353
                     /gene="thiB"
                     /locus_tag="LGJ20_002234"
     CDS             24370..25353
                     /gene="thiB"
                     /locus_tag="LGJ20_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBK2386288.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAANWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            25329..26939
                     /gene="thiP"
                     /locus_tag="LGJ20_002235"
     CDS             25329..26939
                     /gene="thiP"
                     /locus_tag="LGJ20_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBK2386289.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AIWQDSYLCHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            26923..27621
                     /gene="thiQ"
                     /locus_tag="LGJ20_002236"
     CDS             26923..27621
                     /gene="thiQ"
                     /locus_tag="LGJ20_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBK2386290.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(27735..28499)
                     /locus_tag="LGJ20_002237"
     CDS             complement(27735..28499)
                     /locus_tag="LGJ20_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2386291.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(28585..29463)
                     /gene="araC"
                     /locus_tag="LGJ20_002238"
     CDS             complement(28585..29463)
                     /gene="araC"
                     /locus_tag="LGJ20_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBK2386292.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            29802..31502
                     /gene="araB"
                     /locus_tag="LGJ20_002239"
     CDS             29802..31502
                     /gene="araB"
                     /locus_tag="LGJ20_002239"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBK2386293.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            31513..33015
                     /gene="araA"
                     /locus_tag="LGJ20_002240"
     CDS             31513..33015
                     /gene="araA"
                     /locus_tag="LGJ20_002240"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK2386294.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            33184..33879
                     /gene="araD"
                     /locus_tag="LGJ20_002241"
     CDS             33184..33879
                     /gene="araD"
                     /locus_tag="LGJ20_002241"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK2386295.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            33954..>35896
                     /gene="polB"
                     /locus_tag="LGJ20_002242"
     CDS             33954..>35896
                     /gene="polB"
                     /locus_tag="LGJ20_002242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBK2386296.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQ"
BASE COUNT         8705 a         9974 c         9172 g         8045 t
ORIGIN      
        1 ccttttccac gcagaccaga gatacgaatg aaatcaattt cgatgccatg ttttggcact
       61 aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc ctgagccatt
      121 agatggtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc tgccatcacc
      181 attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc gctttctcca
      241 gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata atcagtaagc
      301 tcgaaccacc gtaactgatc agcggcaatg tcagaccttt ggtcggtaac atccccgccg
      361 ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa caggcgagaa
      421 aaccggaaaa acggtggtca atttctaacg ctttgcggcc aatcgacatc gcgcgaaaag
      481 cgacgaagaa taccattaaa agtgccagca ccacaccgac ataccccagt tcttcgccga
      541 taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt
      601 tacctaaacc ttgtccccaa agttcgccgc gaccaaacgc catcagcgat tgcgttaact
      661 gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca cggcggatac
      721 ggtacggttc ggcgagtatc agcaacacaa ccgctgaaat gcccataccg ataatggcaa
      781 tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc acaaacaaca
      841 ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc agaatcacgc
      901 ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta cgcaccagat
      961 agttggcgat ataacaaaac agcgacagtt ttgtcagctc cgcaggctgg atacgcagca
     1021 aaccgagatc gatccaacgc gatgcccctt taaccgagct acccactacc agcacgatca
     1081 tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag aactccatcg
     1141 gcagacgcag cgtaatgatc gccagaatga atgccaggat cagatagacg ccatcacgct
     1201 tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac gccgaggtca
     1261 ccatgataaa gccaatcgcc gcaaggccga aggtcagcca cagtaaggtg cgatcgtaca
     1321 tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcctttt agcgccgtgg
     1381 agatccagac caggatactg aatcctggca ggcgcggcat tttcaggcga gggagagata
     1441 aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt
     1501 cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc ccggctgaac
     1561 acgcggagcc agcaagcgca tcgcctgttc catagtttcg gtttgttctg ccacttccgg
     1621 gcgtagcgcc gccagctgcg caccgtcacg accaaaacag tacaaacgca cgttgtcgcc
     1681 attcagatag cgcgccagtg gactgaagtc cgccgattta ccatcgccgc ccagcaacaa
     1741 atgcagcgtg ccgtctacct gcaagccatt caacgccgct tccgtactgc cgacgttagt
     1801 ggctttcgaa tcgttaatcc agcgtacgcc attgtgctcc agcaccactt caaagcgatg
     1861 cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggca aaccagctgc
     1921 gtctgccagt gccagtgctg ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat
     1981 ctctttcacg ttcagcactt tctcgccttt cactcgcagc caggtttctc cctgctgatg
     2041 attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat cagcaccgcg
     2101 aatcggcatt gttaaggcat catcagcatt gacgacacaa actttcgcat tttcgtaaat
     2161 acgcaatttt gccgcacggt attgctgcaa accaaacgga tagcggtcca tatgatcttc
     2221 agtcacgttc agaatggtcg ccgccaccgc ctgtaagctg gaagtggttt ccagctggaa
     2281 gctcgacagt tccagcacgt acagttcgca ctcagcatca agcagcatca acgctggcag
     2341 gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt cacccactag
     2401 cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg cttgtgcttc
     2461 gcggcagaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa
     2521 ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatctgccg ccatcagcca
     2581 ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat ccaggccagg
     2641 cggtgtcata cgcgtatcca taacgcgcgg cgtcagaccg cgagcgagga aaaagtccac
     2701 gcaggaaagc ccagtgaggc ccaggccgat aatgacgaca tttttaccct gataatcagc
     2761 catgattaac gtaccttcag cgttgccaga ccaatcagaa ccagcatcag cgaaataatc
     2821 cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc
     2881 ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg caggatgaca
     2941 gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgtagc
     3001 agtacggcga taatgcctaa cgcaccacct aacgccagcg aacctacatc gcccataaag
     3061 acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac tatcgcggta
     3121 cagacaataa ccagttcccc tgcgtgtcgc agatacggta tatgcaagta gctggcaaag
     3181 ttcatattgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc
     3241 ataattgcca ggccgtcgag accatcggtc aggtttaccg cgttgccagt acccacaatg
     3301 acgaagtaag ccagcagaat gtagaacagc cccagctgcg gcatcacatc tttaaagaat
     3361 gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacagggc gaaggcgaca
     3421 cccagcgcaa tgaccgacat ccagaaatac ttccaacgag cgatcaaccc tttggtgtct
     3481 ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcacc
     3541 accaacacgc accagacgta cggattggac gggtaagccc acagcagtac ggagatcaca
     3601 atcgccgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat
     3661 tcaggaccgt cgttacgcac cacctgacca aaggaaagtt tttgcaaatg agcaatcata
     3721 cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac
     3781 gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa
     3841 actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggcg gcactacgtg
     3901 aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgtaa
     3961 ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg atagcatggc
     4021 tttgtttacc cacgcttaac acgcggtcaa taccagcagc ttttgccgcc tcgcccacct
     4081 gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc accagcacgc
     4141 ggtagcccgg catttcagcc agtacctgaa cagcggcggt cattgaaccg acattggcgt
     4201 tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac agacggcctg
     4261 gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca
     4321 tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca
     4381 gaacatcgac gctaccggtt ggggtttgta gggtaaattc cgtaccgtgc gaggtcacat
     4441 ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc cacactttgc
     4501 gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc ataatggcga
     4561 taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg
     4621 agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgcagct tccgggcgag
     4681 tcagactcac agtccaggct atttcgccct gatggttcgc gccaagttca ataactgcgt
     4741 aatcgtattc cggcgttaag cgcaacagcg tcatcggtac accgatgtcg ttgttgagat
     4801 tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc atctctttaa
     4861 cggaggtttt gccggaggac cccgtcagag caaccacgcg cgccggaact tgctggcgaa
     4921 cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac tgcggcaggt
     4981 cgatgtccag cggacggcta accagtagtg cgcctgcgcc gccagctttc gcctggtcgg
     5041 caaaatcgtg ggcatcaaaa cgttcgcctt tcagggcaac aaacaggcag cccggcgtca
     5101 gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc agttcaccgt
     5161 tgagaatgtc ggtaagttgg ctaagggtta cgctaatcat gcaatcaccc ccagcagacg
     5221 cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcttc
     5281 atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa cggcgcaagt
     5341 caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca ttcccgccag
     5401 aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc
     5461 cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt tatcgcgatc
     5521 gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa
     5581 ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg gtttgcctgg
     5641 cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg ttttcagcag
     5701 atcagccagt ggatagccga gtgccagcag tgtcgccagc gcgagcagca ggttgctgac
     5761 gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccaacttg agctaaagcg
     5821 aatcgtcgca ccgctgtcgt gatagttcac gtcgatcgct tttaaccagc gtccgtgaca
     5881 gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca
     5941 gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat gttcagaata
     6001 aagcagccat ttcgcagctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg
     6061 gcttaagttg gtaaagaccg acgccgcaaa tttcaatgcc gccacacggt gctgtaccag
     6121 cccgtgggag gaaacttcca ttgcgcaaaa cgtcgcgccc tgctccacca gccccgccag
     6181 ctcatgctga acatcgactg cggaaccggt agtattttct gtcgggatca ctttcccaag
     6241 cagaccgtta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg
     6301 cgccagcagc tgggtagtcg tggttttgcc gttggtgccc gttacgccta cgagacgtaa
     6361 attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct cgttgagctg
     6421 gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccatcgg tcgcctcatc
     6481 tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg
     6541 cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct
     6601 gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag
     6661 gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca aattcatttt
     6721 tatcgcccgt tgtcagcgca tccggctcga tgttcatggt acgcaatacg ccgcccatga
     6781 tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt
     6841 tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag
     6901 caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa
     6961 tggcgatacg atagccttta atcgccgctt tcacgccgcc gccgcctggt agcgccacgc
     7021 tttccatcat atgcaccacg gtgcgaacaa tggattccgg gaagacacgt tcaccgggaa
     7081 ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg
     7141 cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg
     7201 ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca
     7261 accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg
     7321 acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt aataccccgg
     7381 tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta attcgataag
     7441 gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg gtcattacca
     7501 ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg ttacgcatcg
     7561 cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta gccatcgcca
     7621 gcacttcacc ggtgttgaca tccaccagca cggcgctacc ggattcagcc ttgttaaagg
     7681 ccaccgcgtt gttcagttcg cgataaacca gcgcctgcaa gcgttcatca atactcaacg
     7741 ccaggttgtg cgccgcctgg ctgtcagtag aagaaatatc ttcaattacg cgaccatagc
     7801 ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct
     7861 caacgccctc aatcccttga ctatcgacgt tagtaaagcc gatgaggtga gcagtcactt
     7921 cgccggacgg atagtaacgg cgagactctt cacgcagatg aatccccggc agtttcagtt
     7981 ttttgatgta gtccgccatg tcagggttca cctgacgcgc cagataaata aagcgccctt
     8041 tcgggttggc gttaatgcgg gctgaaagct gatccagcgg aatattgagc gcgttagcca
     8101 gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag
     8161 cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca
     8221 tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tctttcacca
     8281 gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca
     8341 ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct
     8401 gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt
     8461 gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcga
     8521 tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt
     8581 caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc agtaaacggg
     8641 tatggtgcgc cgtggttacc acagtcaccg ccgtcaaaat aatgcaaatg aacaggcaga
     8701 gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat gcatggcgct
     8761 cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc
     8821 attcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc
     8881 ttcttcgccc ggcattaact tgcctagtgc tcgcagctga cggccaccca gttttttgag
     8941 ctgctcttca gttatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat
     9001 aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa gccgcccacc
     9061 cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca gttcactgtt
     9121 tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac
     9181 cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg
     9241 gttacgctcg acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt
     9301 caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc
     9361 acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgctc
     9421 agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc
     9481 gataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga
     9541 gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc
     9601 cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc
     9661 aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa cggcttcatc
     9721 cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc
     9781 gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg acctgttgat
     9841 gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt
     9901 ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca
     9961 tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca
    10021 tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca
    10081 gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg accggcagcg ttctcaagca
    10141 gctgttcccg gtaacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg
    10201 ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac
    10261 tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg
    10321 taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgca
    10381 gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca ccgttttaaa
    10441 atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt
    10501 aggacatatc ttccccggtt aacagtccct gttgcgtctg tgtggcgctt gatgccagcg
    10561 ggtttgcgca aaagcaaaga aaagtgcttg ttaaatcggc aattcatctc tgcatgtaac
    10621 aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc agctggtgat
    10681 ttaattaacg ggcgggttta aaagaactta tcaataatag tcaggaatag tcttatttac
    10741 tttaaggata ttgatgtcca ttcccgtact ctacgcgcca gagggaaatt cacctggcgc
    10801 gtatttttgt tcgcggctta gctacggctg agcacaccgc gacgatagag attacgttta
    10861 atgcgcgtta aaccaggttt tggcttacgc ggttcgtcca ggcttgccag gacaatctcc
    10921 agcacacgct ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg gcactgtaag
    10981 aagtcgagca gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg cagtttacca
    11041 tcgcgacgta gcgttacatc catcacccct tgcaacaacg caaacgacgt tgtgaacagc
    11101 gcctgcggca tcggatgcgt ttccagccat ttttcgaata actgggcagc cgcctcccgc
    11161 tcatagctgt tggcatacag gaaatgcact tcgcgcggat catctttcca ggcagtacgg
    11221 aaaccttgtt cacgcaggaa gctgacagaa agctccggca gcgcgccaag ataaagcacc
    11281 gtctcggcgg gaaacttacg taactcttcc gccagcattt cggcatcatc ctgatcggca
    11341 ccaaccacgc tggtgaagtg ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac
    11401 gggtcgttag cccagcgttg ataaaaagga tgctcaggag gcaacgacgt cgaaacaata
    11461 atggcatcga cctgacgctg taaaaggtgc tcaatacagc gcatttcgtt gtctggctga
    11521 tcttctgagc aggcaattag cagttgataa ccccgttgcc gcgcctggcg ttcaagatag
    11581 ttagcgatgc gggtatagct ggtgttctcc agatcgggga tcacaagacc aatagaacgt
    11641 gtgcgtccag cacgaagccc agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc
    11701 acagccatga ctttttcaac ggttttgtcg ctcacacggt attgcttcgc tttgccgtta
    11761 ataacatagc ttgcagtggt ccgcgacact cccgccagcc gagcgatttc atccagtttc
    11821 acaattgccc cttgcgtaaa atgtaaaaac catagccatt gtactggtat gggttaaatt
    11881 cattaacatc taagcgcaga aaattaactg cggcaactgc ttttattcca ggttcccact
    11941 gatttcgcaa aaaaagccca acgtgacatg ttgggctgca aattgcgcac tgagatcatt
    12001 ctcaacgcat tattttatcg ccgcgcgaaa gtccgaccac accagagcga gcaacttcca
    12061 caattttcgc cacatcgcga atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg
    12121 ctaattgaac ggtataaagc gagggggtga catcgataat ttgcccacgg aatatttccg
    12181 tattacgttt cacttcgtca cgcccgtaac cgctggcctg aattttcacc agcatgattt
    12241 cccgctcaac atgcgcgccc tgccccaact cactcacgcg caagacatcg accagcttgt
    12301 gtaattgctt ttcgatctgc tcaagcactt tttcatcgcc cacggtctgg atggtcatac
    12361 gcgataatgt cggatcgtcg gttggcgcaa cggtcaggct ttcaatgttg tagccacgct
    12421 gggaaaaaag gccaatcacg cgggataacg cgcctgattc attttcgagt aagactgata
    12481 atatccggcg cataatcagg ttctctccgt tttgcttaac cacatttcat ccattccgcc
    12541 cccgcgaatc tgcatcgggt agacgtgctc actgccatcg acggtaacat caacaaacac
    12601 caggcgatta ttgcgcacct gttccagcgc ctcgctaagt ttgctttcca gctcatgcgg
    12661 atgagagatc tggatcccga cgtgaccata ggcttccgcc agacggacga aatcgggtag
    12721 tgattgcata taagattgtg aatgacggcc ggaatagatc atgtcctgcc actgtttcac
    12781 catccccaga tagcggttat tgagattcac caccagtacc ggcaactcgt attgcaacgc
    12841 ggtagacagt tcctggatgt tcatctgaat actgccgtcg ccagtgacgc aaaccacggt
    12901 ttcttctggc aacgccattt tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc
    12961 gaggccaccg gaattgatcc agcgacgcgg tttgtcgaat ggataataaa gtgcagcaaa
    13021 catctggtgc tgcccgacat cggacgtcac gtaagcgtct cccttcgtca accgccaaag
    13081 agtctcgatc accgcctgcg gtttaatctt ttcactgtga gtgtcatatt tcaggcactg
    13141 acgagcgcgc cactgttcaa tctgctgcca ccagtcgcgg atctcatcca gcggttgatg
    13201 ggcggattct tgcgacagga gttcaagcat ttgttcgagg acctggcgag catcccccac
    13261 aatcgggata tcagcagtca cggttttaga aatggaagta ggatcaatat cgatatgcag
    13321 aacagtggca tttgggcagt actttgccag attattcgtc gttcggtcat caaatcttac
    13381 cccgacggcg aaaatcacat ccgcgttatg catcgtcata ttggcttcgt aggtgccgtg
    13441 cattcccagc atgcccagcg cctgacgatg cgttgccgga aatgccccca ggcccatcaa
    13501 tgaggaaaca acgggcagat tcaacgcctc caccgtttct ttcaactgct gatggcagcc
    13561 cgccgtgatt gccccaccgc ctacgtagac aaccggtttt tttgccgcca ccagcgtttg
    13621 cagagcacgc ttaatttgcc ctttatgtcc ggtagtagtg ggattgtaag aacgcatact
    13681 aaccgactcc ggccagacat agggtaattt gttcgccgga ttaagaatat ctttcggtaa
    13741 atcaacgact actggtccag gacgaccact tgccgccagc cagaaagcct ttttcagcac
    13801 ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt ttaaccaccg gtcgcgaaat
    13861 ccccaccatg tcgcactcct gaaaggcatc gtaacctatc aacgaggtcg ctacctgccc
    13921 ggaaaggaca actaatggaa tggaatccat ataagcggtg gcgatgccgg taatcgcatt
    13981 ggtcgcccct ggacccgacg ttaccagcac gacgccgact tccccggtcg cacgcgccag
    14041 gccatcggcc atatgcaccg ccgcctgctc atgacgaact aatacatgat caataccacc
    14101 cacggtatgc aatgcatcgt aaatatcaag gactgcgcct ccgggataac cgaatacttg
    14161 tttaacgccc tgatcgataa gcgatcggac gaccatctcg gctccagaca acatctccat
    14221 ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct
    14281 taagcaattg gcagccagag acataaacgc agggtaataa gaataaacag gaggaaaggt
    14341 gaaaagaaac gataaaatcc tccattacga aggattaaat tgacaataaa tcggctgaat
    14401 cccacaactt acagcaattt ctctattttt ttaattaatc ctaaaataga gagcacacac
    14461 attcagaata aaattctaca ttcaataaaa aatgcagaat aaaccagaca ttcatgtctg
    14521 gcctattctg cattcagtta gcgtttgcaa attgagacta attgttcttc catccactga
    14581 tggcctttat cgcgcccggc agcttcatgc caggagagat aacaggttct gctgttttgt
    14641 tttaacggca gcggtaatac ctgtaattct aaggattcag cgaactcttc agccagccaa
    14701 cgcggcgcaa tagcgaccaa atgcgtttgc gacaccacgc taagtacgct catcattgcc
    14761 atgccctgat acgcgatact ggcttgctta tctaccgtgt cataccaagg ttgactaaat
    14821 gacgcgaaac gatcgagcga aaccgccgca tgttgttcgt tataaacatc atgtttcagt
    14881 aacgggccct taattgttgg atgatttttg ctggctacca gcaccatttc atctttaaat
    14941 aatggcacgc tggtaaattc aggacgatgg aagtcttcat aactaatcac aaactccgtt
    15001 tcctgataac gcagctgatg ttcagtgttc tgatttaatg aagacttgaa cataacatgt
    15061 atatttggcg caatctgctc aatgtgatta taaatctgcg aggtcagaat gctgtctaac
    15121 gggctgcaaa cacaaagatg aaatacacgt tcactgctcg cgggttcaaa acctgaacca
    15181 ggcaattcat tttgtactag ttgcaatgcc tgacgaactg agccaaaaag ttgaaatgcg
    15241 cgagcagtcg gttgaatacc acggccataa cgaacaaaaa gctcgtcatt aaacatcacc
    15301 ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca tacccagaac atgagcggca
    15361 cgggtaatgt tttgctcctg catcacggca tcgaaaacgg ttaataagtt gagatcgacc
    15421 atgcgtagct gtggcttgct taactccgcc gtctctgaat gatctgtttg tacctctggc
    15481 atacttaact ccactgtcac acttaactcc ctttccctta ttggaatgca gagaaataat
    15541 cctgaagaat atgatttatc atgcatataa aataagaaaa agcaaaatga gtaaaattcg
    15601 gaaaacataa agatacgggt tctgataaaa acagaatcca ttgccgtgga atgagtcatt
    15661 taccacagca taataatcca taatgttttt gttgcgaaaa caatctaatc ataactaccg
    15721 cgaatactca atcatctaca aaatggatta aatatgctta tttaataatt aatttatata
    15781 tttaatgcat taattcttaa cattaattga tcaataatat tcaccaaatc aatatcaaaa
    15841 aaaatcgcaa aacatataat tcaatacaaa tcatcaggat aggttttgca acgcgtgcat
    15901 tttgtcccct ttttcctcgt tgattagatg caaaaattta tgctgaaata tgtcaaccga
    15961 tgaaaagcgt cggtagttaa gcagaaatta atatcgctta ctttaaccac cgcagcacaa
    16021 ttagctaatt ttacggatgc agaactcacg ctggcgggac gtttttattg cgtcagggtt
    16081 gacatccgtt tttgtatcca gtaactctaa aagcatatcg cattcatctg gagctgattt
    16141 aatgactcac atcgttcgct ttatcggtct actactacta aacgcatctt ctttgcgcgg
    16201 tagacgagtg agcggcatcc agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc
    16261 attgcgcggg tttttttatg cccgaagcga ggcgctctaa aagagacaag gacccaaacc
    16321 atgagccagc aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag
    16381 gcaagcttga gtgtgaaaga aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt
    16441 gacgtgatgg aagtcggttt ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc
    16501 atcgcccgcc aggttaaaaa cagccgcgta tgtgcgttag ctcgctgcgt ggagaaagat
    16561 atcgacgtgg cggccgaatc cctgaaagtc gccgaagcct tccgtattca tacctttatt
    16621 gccacttcgc caatgcacat cgccaccaag ctgcgcagca cgctggatga agtgatcgaa
    16681 cgcgctatct atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc
    16741 gaagatgccg ggcgtacacc cattgccgat ctggcgcgag tggtcgaagc ggcgattaat
    16801 gccggtgcca ccaccatcaa cattccggac accgtgggct acaccatgcc gtttgagttc
    16861 gccggaatca tcagcggcct gtatgaacgc gtacctaaca tcgacaaagc cattatttcc
    16921 gtacataccc acgacgattt aggcctggcg gtcggcaact cactggcggc ggtacatgcc
    16981 ggtgcacgcc aggtggaagg tgcaatgaac gggatcggcg agcgtgccgg taactgttcg
    17041 ctggaagaag tcatcatggc gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc
    17101 attaatcacc aggagatatg gcgcaccagc cagttagtta gccagatttg taatatgccg
    17161 atcccggcaa acaaagccat tgttggcagc ggcgcattcg ctcactcctc cggtatccac
    17221 caggatggcg tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt
    17281 ctgaaccaaa tccagctgaa tctgacctct cgttcggggc gtgcggcggt gaaacatcgc
    17341 atggatgaga tggggtataa agaaagtgaa tataatttag acaatttgta cgatgctttc
    17401 ctgaagctgg cggacaaaaa aggtcaggtg tttgattacg atctggaggc gctggccttc
    17461 atcggtaagc agcaagaaga gccggagcat ttccgtctgg attacttcag cgtgcagtct
    17521 ggctctaacg atatcgccac cgccgccgtc aaactggcct gtggcgaaga agtcaaagca
    17581 gaagccgcca acggtaacgg tccggtcgat gccgtctatc aggcaattaa ccgcatcact
    17641 gactataacg tcgaactggt gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg
    17701 ctgggtcagg tggatattgt cgccaactac aacggtcgcc gcttccacgg cgtcggcctg
    17761 gccaccgata ttgtcgagtc ctccgccaaa gccatggtgc acgtactgaa caatatctgg
    17821 cgtgccgcag aagtcgaaaa agagttgcaa cgcaaagctc aacacaacga aaacaacaag
    17881 gaaaccgtgt gatgtcgaag aattaccata ttgccgtatt gccgggggac ggtattggtc
    17941 cggaagtgat gacccaggcg ctgaaagtgc tggatgccgt gcgcaaccgc tttgcgatgc
    18001 gcatcaccac cagccattac gatgtaggcg gcgcagccat tgataaccac gggcaaccac
    18061 tgccgcctgc gacggttgaa ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag
    18121 gcggtccgaa atgggaacat ttaccaccag accagcaacc agaacgcggc gcgctgttgc
    18181 ctttgcgtaa gcacttcaaa ttattcagca acctgcgccc ggcaaaactg tatcaggggc
    18241 tggaagcatt ctgtccgctg cgtgcagaca ttgccgctaa cggcttcgac atcctgtgcg
    18301 tgcgcgaact gaccggcggc atctatttcg gtcagccaaa aggccgcgaa ggtagcggac
    18361 aatatgaaaa agcgtttgat accgaggtgt atcaccgttt tgagatcgag cgtattgccc
    18421 gcatcgcgtt tgaatctgct cgtaaacgtc gccacaaagt cacctcaatc gacaaagcta
    18481 acgtgctgca atcctctatt ttatggcgtg aaatcgtcaa cgagatcgcc acggaatacc
    18541 cggatgtcga actggcgcat atgtacatcg acaacgccac catgcagctg attaaagatc
    18601 catcacagtt tgacgttctg ctgtgctcca acctgtttgg cgacattctg tctgacgagt
    18661 gcgcaatgat cactggctcg atggggatgt tgccttccgc cagcctgaac gagcaaggtt
    18721 ttggtctgta tgaaccggca ggcggctcag caccagatat cgcaggcaaa aacatcgcca
    18781 acccgattgc gcaaattctg tcgctggcac tgctgctgcg ctacagcctg gatgccgatg
    18841 atgcggcttg cgccattgaa cgcgccatta accgcgcatt agaagaaggc attcgcaccg
    18901 gggatttagc ccgtggcgct gccgccgtta gtaccgatga aatgggcgat atcattgccc
    18961 gctatgtggc agaaggggtg taatcatggc taaaacgtta tacgaaaaat tattcgacgc
    19021 tcacgtagtg tacgaagccg aaaacgaaac cccgctgtta tatatcgacc gccacctggt
    19081 gcatgaagtg acctcaccgc aggcgtttga tggtctgcgc gcccacggtc gcccggtacg
    19141 tcagccgggc aaaaccttcg ccactatgga tcacaacgtc tctacccaga ccaaagacat
    19201 taatgcctgt ggtgaaatgg cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga
    19261 atttggcgtc gagctgtatg acctgaatca cccgtatcag gggatcgtcc acgtaatggg
    19321 gccggaacag ggcgtcacct tgccggggat gaccattgtc tgcggcgact cgcataccgc
    19381 cacccacggc gcgtttggcg cactggcctt tggtatcggc acttccgaag ttgaacacgt
    19441 actggcaaca caaaccctga aacagggccg tgcgaagacc atgaaaattg aagtccaggg
    19501 caaagccgcg ccgggcatta cagcaaaaga tatcgtgctg gcaattatcg gtaaaactgg
    19561 cagcgcaggc ggcaccgggc atgtggtgga gttttgcggc gaagcaattc gtgatttaag
    19621 catggaaggt cgtatgaccc tgtgcaatat ggcaatcgaa atgggcgcaa aagccggtct
    19681 ggttgcaccg gacgaaacca cctttaacta tgtcaaaggc cgtttgcatg cgccgaaagg
    19741 caaagatttc gacgacgccg ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac
    19801 tttcgatacc gttgtcactc tgcaagcaga agaaatttca ccgcaggtca cctggggcac
    19861 caatccaggc caggtgattt ccgtgaacga caatattccc gatccggctt cgtttgccga
    19921 tccggttgaa cgcgcgtcgg cagaaaaagc gctggcctat atggggctga aaccgggcat
    19981 tccgctgacc gaagtggcta tcgacaaagt gtttatcggt tcctgtacca actcgcgcat
    20041 tgaagattta cgcgcggcag cggagatcgc caaagggcga aaagtcgcgc caggcgtgca
    20101 ggcactggtg gttcccggct ctggcccggt aaaagcccag gcggaagcgg aaggtctgga
    20161 taaaatcttt attgaagccg gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc
    20221 gatgaacaac gaccgtctga atccgggcga acgttgtgca tccaccagca accgtaactt
    20281 tgaaggccgc caggggcgcg gcgggcgcac gcatctggtc agcccggcaa tggctgccgc
    20341 tgctgctgtg accggacatt tcgccgacat tcgcaacatt aaataaggag cacaccatgg
    20401 cagagaaatt tatcaaacac acaggcctgg tggttccgct ggatgccgcc aatgtcgata
    20461 ccgatgcaat catcccgaaa cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc
    20521 atctgtttaa cgactggcgt tttctcgatg aaaaaggcca acagccaaac ccggacttcg
    20581 tgctgaactt cccgcagtat cagggcgctt ccattttgct ggcacgagaa aacttcggct
    20641 gtggctcctc gcgtgagcac gcgccctggg cattgaccga ctacggtttt aaagtggtga
    20701 ttgcgccgag ttttgctgac atcttctacg gcaatagctt taacaaccag ctgctgccgg
    20761 tgaaattaag cgatgcagaa gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga
    20821 tccatttcga cgtggatctg gaagcgcaag aggtgaaagc gggagagaaa acctatcgct
    20881 ttaccatcga tgccttccgc cgccactgca tgatgaacgg tctggacagt attgggctta
    20941 ccttgcagca cgacgacgcc attgccgctt atgaagcaaa acaacctgcg tttatgaatt
    21001 aatccccttg cccggtcaaa tgaccgggct ttccgctatc gtccacgtca tcaaacgtct
    21061 ttaacctttg cggttaaaaa taatgcgaca acagaaataa ccgcaattac ccagtagaca
    21121 gcaaagtgcc cccaactttg tgcaattgct ccctgaataa cgccagccag aattaccccg
    21181 gtagaaatac tgttagtaaa taaggtggta gctgcccccg ctcttccagg cattaaatcc
    21241 tgaaaccata gcatcccaat acccgcaaca atgccgataa atacagcgtt aaaaagttgc
    21301 agcgtcatca acgccatacg gctatgaaag aaaatcaatc cggtgtaaaa cagtactcct
    21361 gccgccactg ctatgaccat cattcgccgc ttaccataac gtttgacata gtagccagcc
    21421 agaatcattg ctggtatttc cagtccagct gccgtcccca tcaggaaacc cgccagtttg
    21481 tctggcaatc ctaactcgct actgatccac aacggcatat caataatgta catggtgttg
    21541 caggtccaca ttaacgtcga ggcgacaaat aacatccgta cgttactatc ctgccagcca
    21601 ccttgcattg ataaagcatt ttccgacggc agttctaccc gcgccacaga cggaagcata
    21661 aatgcaatca ataccagact gagtgtgaat atcccggcgg caatcgaaaa catcaccgta
    21721 aagccgtaat tcaacgccag cataaaggcc aacggtggac cgataaccca tgccagagaa
    21781 agctgcgcac gcatcaccga gctaaacatc accacttctc gcgccgagtt atccgcatat
    21841 tcccgcgcca gagcaaataa ctgtggcatt gccgtattgg ccagagatgc cagaagcaca
    21901 ccacaggtga taagcgtcag ataatgacga ttaaatgcaa acaatagcgc attgccgata
    21961 gccatcaaac agcaaaatat aatcagtttt cgccgatcgc cctgactgtc agaacgtttt
    22021 gccaaccaga ggcttacgcc gatcccagca atagcattca ccgtataaaa gagtccgatc
    22081 cagaaaggtt gcgcgccaac ctcacgactc agaaataagc tcaatgtagg agcctgtagc
    22141 gccccggcca cccccatcat aaaagcgacc agcataaatg ccgcgtaaac accgttcata
    22201 cggcgagcca tcgtcattat ccagatcata cgttcccttt ttagcgcggc gagaataaaa
    22261 aaaaccagca ggtataatct gctggcgggt gattttacac caatactcag tcacacatga
    22321 tgcaggcaag tcaactttca gaattgcggt catcccactg catcagtcct tcaagcatgg
    22381 ttaatcgttg tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat
    22441 agtgctgata aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact
    22501 taatataggg aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg
    22561 ctcgtctgca acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca
    22621 caacgctcaa cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgtaccctgc
    22681 tcaacaccat gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgcggtaaac
    22741 gctcgcgtct gacattcctc tataccgggc tggcgcttca gcaacagcgg gcggaagacc
    22801 tgctggagca ggatcgtatc gaccaactag tgcagttggt tggcgacaaa gcgactgtgc
    22861 ggcaaatgct ggtttctcat ctgggccgca gcttccgcca ggggcggcac atcctgcgcg
    22921 tgctctacta tcgtccgttg cgtaatctgc tacctggcag cgcattgcgc cgttccgaaa
    22981 cccatattgc ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac
    23041 tggaagcaga catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt
    23101 tgcgtccagg agtccatttt catcatggtc gtgaactgga aatggacgac gtgatcgcct
    23161 ctttaaaacg aatcaatacg ctgccgctct attcgcatat tgcggacatt gtgtcgccga
    23221 cgccctggac gctggatatc catctcacgc aaccggaccg ctggttaccg ttactgctgg
    23281 gacaagttcc ggcgatgatc ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc
    23341 atcccatcgg caccggtccg tatgcggtga tccgcaacac caccaatcaa ctgaaaattc
    23401 aggcattcga tgacttcttc ggttaccggg cattaatcga tgaagttaac gtctgggttc
    23461 tgccggaaat tgccgacgag ccagctggag gtctgatgct aaaaggtcca cagggcgagg
    23521 aaaaagagat tgaaagccgc ctggaggaag gttgctacta tttactgttc gacagccgca
    23581 cccatcgcgg ggcgaatcag caagtcaggg actgggtaag ctatgtgctt tctccaacta
    23641 atctggtcta tttcgctgag gaacagtacc agcaactgtg gttcccggct tatggactgc
    23701 tcccccgttg gcaccatgcc cgcaccataa agagcgaaaa accggctggc ctggaaagcc
    23761 tcaccctgac cttttatcag gatcacagtg agcatcgggt gattgccggg atcatgcagc
    23821 agattctggc aagtcaccag gtcacgctgg aaatcaaaga gatcagctac gatcagtggc
    23881 atgaaggaga gatcgagagc gatatctggc ttaatagcgc caactttacg ctgccgctgg
    23941 atttttcgct gttcgcgcac ttgtgcgagg tgccgctgct ccaacactgt attccgatcg
    24001 actggcaagc cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc
    24061 agcaactggt cgccagtaaa gcaatggtgc cgcttatcca ccactggctg atcattcagg
    24121 ggcaacgcag tatgcgcggc ctgcgcatga ataccctcgg ctggttcgat tttaaatcag
    24181 cgtggtttgc gccaccggat ccatgattgc tggttgataa caaaatcact acactaacgc
    24241 cgttctcaac ggggtgccac gcagacgcgt gcgctgagaa aatacccgtc gaacctgatc
    24301 cggataacgc cggcgaaggg atttgaggct ccttctcaag tcctttgcca ctcttttttg
    24361 aggtgcaaag tgttaaaaaa atgtctgccc ctgctgttgc tgtgcacagc gcccgttttc
    24421 gctaaacccg ttctgactgt ttatacctac gattccttcg ccgccaactg ggggccaggc
    24481 ccggtggtaa aaaaagcctt tgaagccgac tgtaattgcg aactgaaact ggtggcgctg
    24541 gaagatggcg tttcgcttct caaccgtctg cgcatggaag gcaaaaacag taaagccgat
    24601 gtggtgctgg ggctggataa caacctgtta gacgccgcca gtaaaaccgg actatttgcc
    24661 aaaagcggcg tggcagcgga tgccgttaac gttcccggcg gctggaataa tgacactttc
    24721 gtaccgtttg attacggcta tttcgccttc gtctatgaca agaacaaact gaaaaatccg
    24781 ccgcaaagcc tgaaagagct tgttgagagc gatcaaaact ggcgggtgat ttatcaggat
    24841 ccgcgcacca gtacgccggg gctgggcctg ctgttgtgga tgcaaaaagt ctatggcgat
    24901 gacgccccac aagcctggca gaaactggcg aagaaaacgg tcacggtcac caaaggctgg
    24961 agcgaagcct acggcctgtt tttaaaaggt gaaagcgatc tggtgctgag ttacaccacc
    25021 tctccggctt atcacattct cgaagagaaa aaagataact acgccgccgc gaactttagc
    25081 gaaggtcact atctgcaggt ggaagtcgcc gcccgcaccg ctgccagcaa gcagccggag
    25141 ctggcgcaaa aattcctcca gtttatggtt tctccggctt ttcagaatgc gatcccaacc
    25201 ggcaactgga tgtatccggt ggcaaacgtc acgctgcctg ccggttttga acaattgacc
    25261 aaacccgcaa ccacgctgga gttcacgcca gccgaagtgg cggcacaacg tcaggcatgg
    25321 attagcgaat ggcaacgcgc cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa
    25381 gcgccgccac gctggtggta gcggttgcgc tggcggcgtt tctcgccctg tggtggaacg
    25441 cgccgcaggg taactgggtg gcaatctggc aggacagcta cctgtgtcat gtggtgcgct
    25501 tctccttctg gcaggcgttt ctctcggcgc tactctctgt cgtacccgcg atattcctcg
    25561 cccgcgccct ctatcgcagg cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa
    25621 tgaccttgat cctcccggtg ctagttgctg ttttcggcat tcttagcgtc tatggtcgtc
    25681 agggctggct ggcatcgctc tgccaatcgc tcggtctgga gtggaccttt tcgccctacg
    25741 gcctgcaagg cattttgctg gcgcacgtat tttttaatct gccgatggcg agccgcttat
    25801 tactccaggc actggaaaac attcccggcg aacaacgtca gcttgccgcc cagcttggga
    25861 tgcgcggctg gcattttttc cgcttcgtcg aatggccgtg gctacggcga caaatcccgc
    25921 cggttgccgc gcttatcttt atgctctgtt tcgccagctt cgccaccgtg ctatcgctgg
    25981 gcggcggtcc gcaggcaact actatcgagc tggctattta tcaggcgctg agttacgact
    26041 acgatcctgc ccgcgcggcg atgctggcgc tgctccagat ggtgtgttgc cttgggctgg
    26101 tgctgttgag tcagcgacta agtaaggcca ttgcgcccgg taccacgctg ctgcaaggct
    26161 ggcgcgatcc ggacgatcgt ctgcatagcc gcatttgcga cacggtgtta atagtgctgg
    26221 cgctgctgct gttgctgcca ccgttgctgg cggtgatcgt cgatggggta aatcgccagt
    26281 tgccggaagt gctggcacaa ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg
    26341 cgctggcggc aggtgtattg tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg
    26401 aactgcgggc gcggcagaaa atgctggcgg gtcaggcgct ggagatgagc ggcatgttga
    26461 tcctcgccat gccggggatt gtgctggcta ccggcttctt tttactgctc aacaacacca
    26521 ttggcctgcc gcaatctgct gacggcattg tgattttcac caatgcgtta atggcgatcc
    26581 cttatgcgct gaaagtactg gaaaacccga tgcgcgatat caccgcccgc tacagtatgt
    26641 tgtgtcagtc gctgggcatt gaaggctggt cgcgcttaaa agtggtcgag ctgcgcgccc
    26701 tgaaacgtcc actggcgcag gcgctggctt ttgcctgcgt gctgtcgatt ggtgattttg
    26761 gcgtagtggc gttgttcggt aacgatgatt tccgtaccct gccgttttat ctctaccagc
    26821 aaattggctc ctatcgcagc caggacggcg cggtcaccgc gttaattctg ctactactct
    26881 gttttctgct gtttaccgtg attgaaaaac taccggggcg aaatgttaaa actgactgat
    26941 atcacctggc tttatcacca tttgccgatg cgttttagct taacggtaga acgcggcgag
    27001 caggtggcaa tcctcgggcc aagcggcgcg ggtaaaagta ccctgctgaa tttgattgcc
    27061 ggttttctga cgccagccag cggttcgctg actatcgaca gcgttgatca caccacgacg
    27121 ccgccgtcac gccgcccggt atcgatgctg tttcaggaga acaacctgtt cagccacctg
    27181 acggtcgcgc agaacatcgg gctggggctt aatccgggat tgaagctgaa cgcggcgcag
    27241 caggagaaaa tgcacgctat cgcccgccag atggggattg ataatttaat ggcgcggtta
    27301 ccgggcgagc tttccggcgg tcagcgacag cgcgtggcgt tagcgcgttg tctggtgcgc
    27361 gaacagccga ttttattgct cgatgaaccg ttctctgcgc tcgatccggc gttacgtcag
    27421 gagatgctga cgctggtgag cacgagctgc cagcagcaaa aaatgacgct attgatggtg
    27481 tcgcacagtg tggaagatgc ggcgcggatc gccacacgct cggtggtggt cgccgacggg
    27541 cgcatcgcct ggcagggtaa gaccaatgag ttgttgagcg gtaaggcgag tgcttcggca
    27601 ctattgggga ttacgggtta gtatgcactg tcggatgcgg cgtgaacgcc ttatccgacc
    27661 tacgggtagt gcgttttgta ggcatgataa gacgcggtca gcgtcgcatc aggcaacccg
    27721 cacaagacta tctcctaacc cccaaccact ttacgcaaaa tatcgatata caccggcatc
    27781 aacgggtggc gaattaacac caccagcgcc accacgccga tggcagaaat taacggcgtc
    27841 agccacaaca aacggccgcg cgacaaataa tgacttaaac gatcggttgc tttaccgctg
    27901 cgccataacc gccagcacag ccagccacca acccacaaaa ataccgccgt cgccagcagc
    27961 aaccatttaa actcgccgct ctgcattccg gctggaatat cgatcgccgc gcccgccaga
    28021 atcccaggca ggaagtaaaa cggtggccac agcaggcagc caataatatt cggcgtaatg
    28081 aattttgcca ccggcagatc cagcattccc gccaccattg gcaccagcgg acgcgtcggg
    28141 ccaacaaagc gaccgaccag aatggtgaac atgctgtgtt gatgcaacgc atgttcagtt
    28201 ttatcgagta atgctttgtt tttcttcaga aatgaccagc gatgcaacgg ctttttaaaa
    28261 cgccagccca gccagaagga gatccagtcg cccagcagac aaccgacaat ccctgccagc
    28321 caggcgtgcc agaaacttaa ctcgccgctg ccaatcagcg ctcccagccc cgccatcagc
    28381 accgtaccgg gtagaatcaa accgaccagc gccagcgact ccagaaaggc caccaacacc
    28441 accgccatca atgaatacac ggtagattgg gtaataaagt gttccagcaa tgcttgcata
    28501 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc
    28561 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc
    28621 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atactcgcga
    28681 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt
    28741 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct
    28801 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggtgacaagc aaacatgctg
    28861 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc
    28921 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg
    28981 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg
    29041 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg
    29101 gcgaaagaaa cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc
    29161 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
    29221 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
    29281 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
    29341 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
    29401 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
    29461 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca
    29521 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc caaccggtaa ccccgcttat
    29581 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt
    29641 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc
    29701 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
    29761 tactgtttct ccatacccgt ttttttggat ggagtgaaac gatggcgatt gcaattggcc
    29821 tcgattttgg cagtgattct gtgcgagctt tggcggtgga ctgcgccagc ggtgaagaga
    29881 tcgccaccag cgtagagtgg tatccccgtt ggcaaaaagg gcaattttgt gatgccccga
    29941 ataaccagtt ccgtcatcat ccgcgtgact acattgagtc aatggaagcg gcactgaaaa
    30001 ccgtgcttgc agagcttagc gtcgaacagc gcgcagctgt ggtcgggatt ggcgttgaca
    30061 caaccggctc gacgcccgca ccgattgatg ccgacggtaa cgtgctggcg ctgcgcccgg
    30121 agtttgccga aaacccgaac gcgatgttcg tattgtggaa agaccacact gcggttgaag
    30181 aagcggaaga gattacccgt ttgtgccacg cgccgggcaa cgttgactac tcccgctaca
    30241 ttggcggtat ttattccagc gaatggttct gggcaaaaat cctgcatgtg actcgccagg
    30301 acaccgccgt ggcgcaatct gccgcatcgt ggattgagct gtgcgactgg gtgccagctc
    30361 tgctttccgg taccacccgc ccgcaggata ttcgtcgcgg acgttgcagc gccgggcata
    30421 agtcgctatg gcacgaaagc tggggtggct tgccgccagc cagtttcttt gatgagctgg
    30481 acccgatcct caatcgccat ttgccttccc cgctgttcac tgacacctgg actgccgata
    30541 ttccggtggg caccttatgc ccggaatggg cgcagcgtct cggcctgcct gaaagcgtgg
    30601 tgatttccgg cggcgcgttt gactgccata tgggcgcagt tggcgcaggc gcacagccta
    30661 acgcactggt aaaagttatc ggtacttcca cctgcgacat tctgattgcc gacaaacaga
    30721 gcgttggcga gcgggcagtg aaaggtattt gcggtcaggt tgatggcagc gtggtacctg
    30781 gatttatcgg tctggaagca ggccaatcgg cgtttggtga tatctacgcc tggtttggtc
    30841 gcgtactcgg ctggccgctg gaacagcttg ccgcccagca tccggaactg aaagcgcaaa
    30901 tcaacgccag ccagaaacaa ctgcttccgg cgctgaccga agcatgggcc aaaaatccgt
    30961 ctctggatca cctgccggtg gtgctcgact ggtttaacgg ccgccgcaca ccgaacgcta
    31021 accaacgcct gaaaggggtg attaccgatc ttaacctcgc taccgacgct ccgctgctgt
    31081 tcggcggttt gattgctgcc accgcctttg gcgcacgcgc aatcatggag tgctttaccg
    31141 atcaggggat cgccgtcaat aacgtgatgg cgctgggcgg catcgcgcgg aaaaaccagg
    31201 tcattatgca ggcctgctgc gacgtgctga atcgcccgct gcaaattgtt gcctctgacc
    31261 agtgctgtgc gctcggtgcg gcgatttttg ctgccgtcgc cgcgaaagtg cacgcagaca
    31321 tcccatcagc ccagcaaaaa atggccagtg cggtagagaa aaccctgcaa ccgcgcagcg
    31381 aacaggcaca acgctttgaa cagctttatc gccgctatca gcaatgggcg atgagcgccg
    31441 aacaacacta tcttccaact tccgccccgg cacaggctgc ccaggccgtt gcgactctat
    31501 aaggacacga taatgacgat ttttgataat tatgaagtgt ggtttgtcat tggcagccag
    31561 catctgtatg gcccggaaac cctgcgtcag gtcacccaac atgccgagca cgttgttaat
    31621 gcgctgaata cggaagcgaa actgccctgc aaactggtgt tgaaaccgct gggcaccacg
    31681 ccggatgaaa tcaccgctat ttgccgcgac gcgaattacg acgatcgttg cgctggtctg
    31741 gtggtgtggc tgcacacctt ctccccggcc aaaatgtgga tcaacggcct gaccatgctc
    31801 aacaaaccgt tgctgcaatt ccacacccag tttaacgcgg cgctgccgtg ggacagtatc
    31861 gatatggact ttatgaacct gaaccagact gcacatggcg gtcgcgagtt cggcttcatt
    31921 ggcgcgcgta tgcgtcagca acatgccgtg gttaccggtc actggcagga taaacaagcc
    31981 catgagcgta tcggctcctg gatgcgccag gcggtctcta aacaggatac ccgtcatctg
    32041 aaagtctgcc gttttggcga taacatgcgt gaagtagcgg tcaccgatgg tgataaagtt
    32101 gccgcacaga tcaagttcgg tttctccgtc aatacctggg cggttggcga tctggtgcag
    32161 gtggtgaact ccatcagcga cggcgatgtt aatgcgctgg tcgatgagta cgaaagctgc
    32221 tacaccatga cgcctgccac acaaatccac ggcgaaaaac gacagaacgt gctggaagcg
    32281 gcgcgtattg agctggggat gaagcgtttc ctggaacaag gtggcttcca cgcgttcacc
    32341 accacctttg aagatttgca cggtctgaaa cagcttccag gtctggccgt acagcgtctg
    32401 atgcagcagg gttacggctt tgcgggcgaa ggcgactgga aaaccgccgc cctgcttcgc
    32461 atcatgaagg tgatgtcaac cggtctgcag ggcggcacct cctttatgga ggactacacc
    32521 tatcacttcg agaaaggtaa tgacctggtg ctcggctccc atatgctgga agtctgcccg
    32581 tcgattgccg tagaagagaa accgatcctc gacgttcagc atctcggtat tggtggtaag
    32641 gacgatcctg cccgactgat cttcaatacc caaaccggtc cagcgattgt cgccagcctg
    32701 attgatctcg gcgatcgtta ccgtctgctg gttaactgta tcgacacggt gaaaacaccg
    32761 cactccctgc cgaaactgcc ggtggcgaat gcgctgtgga aagcgcaacc ggatctgcca
    32821 actgcttccg aagcgtggat cctcgctggt ggcgcgcacc ataccgtctt cagccatgcg
    32881 ctgaacctca acgatatgcg ccagttcgcc gagatgcacg acattgaaat cacggtgatt
    32941 gataacgaca cccgcctgcc agcgtttaaa gacgcactgc gctggaacga agtgtattac
    33001 ggatttcgtc gctaagtagc cacatcaggt atgtaacgcc tgatgcgacg ctgacgcgtc
    33061 ttatctggcc tacacgctgc gattttgtag gccggataag caaagcgcat ccggcattgt
    33121 tttccccctc gcccctttgg ggagagggcc ggggtgaggg gaaacgacct ctcaggagtc
    33181 aacatgttag aagatctcaa acgccaggta ttagaagcca acctggcgct gccaaaacac
    33241 aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg cggcgtcttt
    33301 gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat ggtcgtggtt
    33361 agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga cacgccaact
    33421 caccggctgc tctatcaggc attcccctcc attggcggca ttgtgcatac gcactcgcgc
    33481 cacgccacca tctgggcgca gtcgggtcag tcgattccag caatcggcac cacccacgcc
    33541 gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga aatcaacggc
    33601 gaatatgagt gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca gggtatcgat
    33661 gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc cgtttgcatg gggcaaaaat
    33721 gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat ggggatattc
    33781 tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga taaacactat
    33841 ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa aaccacagcc
    33901 aatcaaacga aaccaggcta tactcaagcc tggttttttg atggattttc agcgtggcgc
    33961 aggcaggttt tatcttaacc cgacactggc gggacacccc gcaagggaca gaagtctcct
    34021 tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa gagtccgtgg
    34081 cgtttattcc cgccgatcag gttccccgcg ctcagcatat tttgcagggt gaacaaggct
    34141 ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat ggcctttact
    34201 gtcgcgccca tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt ggcgttaccg
    34261 tctacgaggc cgatgtgcgc ccgccagaac gctatctgat ggagcggttt atcacctcac
    34321 cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt ctgaaaccgc
    34381 atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc acccgccacg
    34441 gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg catcgtttat atgctggggc
    34501 cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc agccgcccgc
    34561 tgctgctgga aaaactcaac gcctggtttg caaaccacga tcccgatgtg atcatcggct
    34621 ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt taccgtcttc
    34681 cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc tttaaaaacg
    34741 gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag gcgctgaaat
    34801 ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag ctattaggcg
    34861 aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc cgtttcgccg
    34921 aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg acgcagatct
    34981 tccacaaaac tgaaatcatg ccatttttac tcgaacgagc aacggtgaac ggcctgccgg
    35041 tggatcggca cggcggttcg gtggcagcgt ttggtcatct ctattttccg cgaatgcatc
    35101 gcgctggtta tgtcgcgcct aatctcggcg aagtgccacc gcacgccagc cctggcggct
    35161 acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac tataaaagcc
    35221 tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtg gaaggcatgg
    35281 cgcagcctga tccagagcac agtaccgaag gttttctcga tgcctggttc tcacgagaaa
    35341 aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa gccaaacgcc
    35401 agggtaacaa accgctgtcg caggcgctga aaatcatcat gaatgccttt tatggcgtgc
    35461 tcggcaccac cgcctgccgc ttcttcgatc cgcggctggc atcgtcgatc accatgcgtg
    35521 gtcatcagat catgcggcaa accaaagcgt tgattgaagc acagggctac gacgttatct
    35581 acggcgatac cgactcaacg tttgtctggc tgaaaggcgc acattcggaa gaagaagcgg
    35641 cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa acgctgcaaa
    35701 aacaacggct gaccagcgca ttagaactgg agtatgaaac ccatttctgc cgttttctga
    35761 tgccaaccat tcgcggagcc gataccggca gtaaaaagcg ttatgccgga ctgattcagg
    35821 agggcgacaa gcagcggatg gtgtttaaag ggctggaaac cgtgcgcacc gactggacgc
    35881 cgctggccca gcagtt
//
LOCUS       DAFATA010000037        35811 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFATA010000037 DAFATA010000000
VERSION     DAFATA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.037"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..101)
                     /locus_tag="LGJ20_002243"
     CDS             complement(<1..101)
                     /locus_tag="LGJ20_002243"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="hydrolyzes the terminal non-reducing
                     N-acetyl-D-hexosamine residues in
                     N-acetyl-beta-D-hexosaminides; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBK2386297.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTR"
     gene            complement(112..936)
                     /gene="thiK"
                     /locus_tag="LGJ20_002244"
     CDS             complement(112..936)
                     /gene="thiK"
                     /locus_tag="LGJ20_002244"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBK2386298.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(917..1558)
                     /gene="lpoB"
                     /locus_tag="LGJ20_002245"
     CDS             complement(917..1558)
                     /gene="lpoB"
                     /locus_tag="LGJ20_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBK2386299.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(1572..1949)
                     /locus_tag="LGJ20_002246"
     CDS             complement(1572..1949)
                     /locus_tag="LGJ20_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBK2386300.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(1952..2311)
                     /gene="hinT"
                     /locus_tag="LGJ20_002247"
     CDS             complement(1952..2311)
                     /gene="hinT"
                     /locus_tag="LGJ20_002247"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBK2386301.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            2645..4834
                     /gene="fhuE"
                     /locus_tag="LGJ20_002248"
     CDS             2645..4834
                     /gene="fhuE"
                     /locus_tag="LGJ20_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBK2386302.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTQGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(4894..6327)
                     /gene="ptsG"
                     /locus_tag="LGJ20_002249"
     CDS             complement(4894..6327)
                     /gene="ptsG"
                     /locus_tag="LGJ20_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBK2386303.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(6622..7419)
                     /locus_tag="LGJ20_002250"
     CDS             complement(6622..7419)
                     /locus_tag="LGJ20_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2386304.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(7430..8434)
                     /gene="holB"
                     /locus_tag="LGJ20_002251"
     CDS             complement(7430..8434)
                     /gene="holB"
                     /locus_tag="LGJ20_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK2386305.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(8431..9072)
                     /locus_tag="LGJ20_002252"
     CDS             complement(8431..9072)
                     /locus_tag="LGJ20_002252"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK2386306.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(9062..10084)
                     /gene="yceG"
                     /locus_tag="LGJ20_002253"
     CDS             complement(9062..10084)
                     /gene="yceG"
                     /locus_tag="LGJ20_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBK2386307.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(10087..10896)
                     /gene="pabC"
                     /locus_tag="LGJ20_002254"
     CDS             complement(10087..10896)
                     /gene="pabC"
                     /locus_tag="LGJ20_002254"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBK2386308.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(11016..12257)
                     /gene="fabF"
                     /locus_tag="LGJ20_002255"
     CDS             complement(11016..12257)
                     /gene="fabF"
                     /locus_tag="LGJ20_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK2386309.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(12345..12581)
                     /gene="acpP"
                     /locus_tag="LGJ20_002256"
     CDS             complement(12345..12581)
                     /gene="acpP"
                     /locus_tag="LGJ20_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK2386310.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(12792..13526)
                     /gene="fabG"
                     /locus_tag="LGJ20_002257"
     CDS             complement(12792..13526)
                     /gene="fabG"
                     /locus_tag="LGJ20_002257"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBK2386311.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(13539..14468)
                     /gene="fabD"
                     /locus_tag="LGJ20_002258"
     CDS             complement(13539..14468)
                     /gene="fabD"
                     /locus_tag="LGJ20_002258"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK2386312.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(14484..15437)
                     /gene="fabH"
                     /locus_tag="LGJ20_002259"
     CDS             complement(14484..15437)
                     /gene="fabH"
                     /locus_tag="LGJ20_002259"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBK2386313.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(15505..16575)
                     /gene="plsX"
                     /locus_tag="LGJ20_002260"
     CDS             complement(15505..16575)
                     /gene="plsX"
                     /locus_tag="LGJ20_002260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK2386314.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(16656..16829)
                     /gene="rpmF"
                     /locus_tag="LGJ20_002261"
     CDS             complement(16656..16829)
                     /gene="rpmF"
                     /locus_tag="LGJ20_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK2386315.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(16881..17402)
                     /gene="yceD"
                     /locus_tag="LGJ20_002262"
     CDS             complement(16881..17402)
                     /gene="yceD"
                     /locus_tag="LGJ20_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBK2386316.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            17601..18185
                     /gene="yceF"
                     /locus_tag="LGJ20_002263"
     CDS             17601..18185
                     /gene="yceF"
                     /locus_tag="LGJ20_002263"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBK2386317.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(18297..19256)
                     /gene="rluC"
                     /locus_tag="LGJ20_002264"
     CDS             complement(18297..19256)
                     /gene="rluC"
                     /locus_tag="LGJ20_002264"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBK2386318.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            19829..23014
                     /gene="rne"
                     /locus_tag="LGJ20_002265"
     CDS             19829..23014
                     /gene="rne"
                     /locus_tag="LGJ20_002265"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBK2386319.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAIEPVVSAPVIEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVTQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(23211..24164)
                     /gene="flgL"
                     /locus_tag="LGJ20_002266"
     CDS             complement(23211..24164)
                     /gene="flgL"
                     /locus_tag="LGJ20_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBK2386320.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGPL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(24176..25819)
                     /gene="flgK"
                     /locus_tag="LGJ20_002267"
     CDS             complement(24176..25819)
                     /gene="flgK"
                     /locus_tag="LGJ20_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBK2386321.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(25885..26826)
                     /gene="flgJ"
                     /locus_tag="LGJ20_002268"
     CDS             complement(25885..26826)
                     /gene="flgJ"
                     /locus_tag="LGJ20_002268"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBK2386322.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(26826..27923)
                     /gene="flgI"
                     /locus_tag="LGJ20_002269"
     CDS             complement(26826..27923)
                     /gene="flgI"
                     /locus_tag="LGJ20_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBK2386323.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(27935..28633)
                     /gene="flgH"
                     /locus_tag="LGJ20_002270"
     CDS             complement(27935..28633)
                     /gene="flgH"
                     /locus_tag="LGJ20_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBK2386324.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(28686..29468)
                     /gene="flgG"
                     /locus_tag="LGJ20_002271"
     CDS             complement(28686..29468)
                     /gene="flgG"
                     /locus_tag="LGJ20_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBK2386325.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(29606..30361)
                     /gene="flgF"
                     /locus_tag="LGJ20_002272"
     CDS             complement(29606..30361)
                     /gene="flgF"
                     /locus_tag="LGJ20_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBK2386326.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(30381..31586)
                     /gene="flgE"
                     /locus_tag="LGJ20_002273"
     CDS             complement(30381..31586)
                     /gene="flgE"
                     /locus_tag="LGJ20_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBK2386327.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(31611..32306)
                     /gene="flgD"
                     /locus_tag="LGJ20_002274"
     CDS             complement(31611..32306)
                     /gene="flgD"
                     /locus_tag="LGJ20_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBK2386328.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(32318..32722)
                     /gene="flgC"
                     /locus_tag="LGJ20_002275"
     CDS             complement(32318..32722)
                     /gene="flgC"
                     /locus_tag="LGJ20_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBK2386329.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(32726..33142)
                     /gene="flgB"
                     /locus_tag="LGJ20_002276"
     CDS             complement(32726..33142)
                     /gene="flgB"
                     /locus_tag="LGJ20_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBK2386330.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALRPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            33297..33956
                     /gene="flgA"
                     /locus_tag="LGJ20_002277"
     CDS             33297..33956
                     /gene="flgA"
                     /locus_tag="LGJ20_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBK2386331.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            34032..34325
                     /gene="flgM"
                     /locus_tag="LGJ20_002278"
     CDS             34032..34325
                     /gene="flgM"
                     /locus_tag="LGJ20_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBK2386332.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            34330..34746
                     /gene="flgN"
                     /locus_tag="LGJ20_002279"
     CDS             34330..34746
                     /gene="flgN"
                     /locus_tag="LGJ20_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBK2386333.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(34786..>35811)
                     /gene="murJ"
                     /locus_tag="LGJ20_002280"
     CDS             complement(34786..>35811)
                     /gene="murJ"
                     /locus_tag="LGJ20_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBK2386334.1"
                     /translation="MIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGML
                     VLPRINFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLM
                     EFPSGVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSG
                     PLTVSLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAI
                     VTLILTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLL
                     RLVVAVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVK
                     EFARRTV"
BASE COUNT         8644 a         9757 c         8942 g         8468 t
ORIGIN      
        1 cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcaa gtatttcacg ctcttccgcg
       61 tccagttcgt aacctttgac atccaacatt actggaccca cactgctctc cttattgttt
      121 tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccggtttg
      181 tcgccagcgg tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct
      241 gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa
      301 ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc
      361 atctccggca tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc
      421 ggcgtggaca tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg
      481 cagacgtttt aacattcgca gccaacccac tgtccgccgc gccggatcgc tttgctgcca
      541 gtacagttcc agtaacggca acagcgttat tcgccagcca aaacgtggtt gttgatgtag
      601 ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg
      661 cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc
      721 gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc
      781 gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac tcccgccact
      841 aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc
      901 gtcgcgcatg atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc
      961 ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc
     1021 tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg
     1081 tacctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg
     1141 cggaaaccag tgtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt
     1201 ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac
     1261 ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc
     1321 agtcataatg gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc
     1381 ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg
     1441 ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac
     1501 acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca
     1561 agattcaccc cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg
     1621 ctgccgtaca gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga
     1681 ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt
     1741 tcttgccttt cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg
     1801 acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc
     1861 tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga
     1921 gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc
     1981 ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc
     2041 ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt
     2101 ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgag acgtcgttca
     2161 cagtcggaat gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat
     2221 cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa
     2281 taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg
     2341 ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct
     2401 ctttcaacct taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc
     2461 cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg
     2521 acaaatatga atgcgtatat ttctcatttg catttacaaa caaaattatt cgcacactaa
     2581 atataatcgc tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt
     2641 tcaaatgctt tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc
     2701 actacttgcc ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc
     2761 cactgaagaa acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga
     2821 ttacagcgta acgtctacct ctgcgggtac caaaatgcag atgactcaac gtgatattcc
     2881 tcagtcggtc actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg
     2941 cgaagtgatg gaaaacacgc aggggatcag caaaagtcag gcggattccg atcgtgctct
     3001 ttattattcc cgcggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt
     3061 tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga
     3121 agtagtgcgt ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa
     3181 tatggttcga aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg
     3241 tagctggaac aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa
     3301 aatccgcgcg cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa
     3361 cagtgaaaag accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct
     3421 ttcagccggt tacgaatatc agcgcattga tgttaatagc cctacctggg gcggtttacc
     3481 gcgctggaat actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga
     3541 ctgggcgtac aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc
     3601 tgatacctgg caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat
     3661 gatgtatgtc gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa
     3721 ttatggacct ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt
     3781 tgatgcgctg gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct
     3841 aatgtttggt ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat
     3901 cttcccggat gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg
     3961 gtcaccacag agcctggcgc aggacgatac cacacatatg aaatcgttat atgctgccac
     4021 tcgtgtcacc cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg
     4081 ggttgatacg ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt
     4141 gtttgacatc aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca
     4201 aaatgatcgt gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct
     4261 gggtctgaaa tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat
     4321 tgagcaggat aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac
     4381 cgcctataaa gcagtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc
     4441 aattaccgac aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga
     4501 aggaaacgcc gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg
     4561 gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta
     4621 taccgacacc gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt
     4681 ggatctcttc acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa
     4741 cctgttcgac aaaacctacg ataccaacgt ggaaggttct atcgtctacg gcgcaccgcg
     4801 taatttcagc attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat
     4861 ctggctgcct tagtctcccc aacgtcttac agattagtgg ttacggatgt actcatccat
     4921 ctcggttttc aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac
     4981 cactacgccc gctgcgccca gtttcttcag gccggcctga tccactttag acacatcagc
     5041 aacgctgacg cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc
     5101 aaatgcagca accagagccg gtgccatttc gctggtacct gtcgcttttg catcttcagt
     5161 cgcgtcttca cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt
     5221 gtagtaaaca atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt
     5281 accagacaga acgatgaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc
     5341 cagaagaata cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat
     5401 cggcgcaacg aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt
     5461 cagcgccgcg gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg
     5521 ccagatagca attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag
     5581 tttacccgca gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc
     5641 tgctgcgttg gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag
     5701 accaaacggt accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt
     5761 ctggtaagca gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa
     5821 ggacagcaca acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt
     5881 accggcaaag aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta
     5941 cgctgcgatc gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga
     6001 ggcgatttct tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac
     6061 catgatgcca taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc
     6121 gagggcgaca ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc
     6181 tgccataaca tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag
     6241 cagaatacct gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg
     6301 caggttagca aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt
     6361 ttacgttctc acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc
     6421 ctttatttat tacacagagt aaaataattc agtgccaatt tgtttgacgg ctatcacgtt
     6481 tcaacttcag atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca
     6541 aacgtggtta ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt
     6601 acgagcttta aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa
     6661 cagacgggcg aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa
     6721 cacagccatg tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg
     6781 cggtaccggc gcaaggtaag gtgagtcagt ttccaccagt aatcgatcca gggggacata
     6841 acgcgcagca tcgcgcagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat
     6901 gtaaaatccg agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag
     6961 tacgccaccg caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcacgggc
     7021 gtcacgggta tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat
     7081 gaaggactct tgctgacgta ctttagtttc tggtgtgtaa taataatcca gcccggtttc
     7141 acccagcgct acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg
     7201 atcattctgg ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac
     7261 cagatcccgc atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac
     7321 atcgcgtgcg gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc
     7381 cagaccatcg agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag
     7441 gaaccggtag cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga
     7501 tgagaagctc gcggttgatg cctgtaacag acattaactg ttcacgaatg tggcaaacat
     7561 cccccagtat agcctgcagg cgcgagggag aaagatggtt tgccagttcg gcgaccaggc
     7621 ccggcacatc aacattagtc acctgcgcag caccatgatg gcgttttagc gcatccatca
     7681 gcaacgttgc cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgctagca
     7741 gcgaatacca gtcgcccgat ggcacgctat atgccaacgc ctgacacaat gtttcacgag
     7801 cctgccagtt atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc
     7861 gcaatgcggc aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg
     7921 cgtactgttc cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca
     7981 gtaaacgttc aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa
     8041 gcgttttcag caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga
     8101 caactttcgc accacctaag cgtgcgtgct cattcagctt ttcggtgacc tcacgtaccg
     8161 catcaatgcc cagcatattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg
     8221 tgccagcctg catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt
     8281 gttggcagag taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta
     8341 acgcctgaat gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt
     8401 cgaaatcagg tcgtaaccat ggataccatc tcatgcgtcc aactccttaa cccagtgggt
     8461 cacggtagtg cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg
     8521 aatactttta tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc
     8581 gaaagactct tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac
     8641 ttccggggta acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc
     8701 acgcagtgtt gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt
     8761 ggagagatcg tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac
     8821 cgtttctacc agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat
     8881 gacttcatcg cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg
     8941 cgtaccgcca ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc
     9001 aaccaccaca ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata
     9061 cttactgcgc atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc
     9121 tggcaagatt ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg
     9181 gcgtttttgc cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac
     9241 ccggcggcaa accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac
     9301 gagaaagttt gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca
     9361 ggcgcatacc aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg
     9421 caacggcggt ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat
     9481 aaggcagacc gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt
     9541 gcgctcgctt gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc
     9601 agaaccaacc ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt
     9661 acttatcgtc gctcagcgta tgcttgatat acggggcctc acgcaattgc ttgaggtaat
     9721 cgctcagacg catcccttct accagtcgca gagggaactg tgcttcttta ccgctttcca
     9781 gcaacttcag catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa
     9841 agtgagaaag atccggttcg atacgcagca gccattgaaa aacccgtggg cgattgatga
     9901 tcttatcagc ataaagctgt tcaccgagcg ccagacgtcc ggtccctggc ttcagggtaa
     9961 atatcgtctc ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc
    10021 ccacaccagc ggcgataccc agtaccacca gcaataacaa gattatcaat aacacttttt
    10081 tcatgactaa ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga
    10141 aaaggagaca tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac
    10201 catctcatct gcctgcaacg cctcttccag agaagcttgc acttcgacaa gctgataaga
    10261 ggattgtgcc agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc
    10321 cagtcgcggc gtataaacta cgttgccctt ccgccagaac aaattagccg cacagcattc
    10381 cgtaacccac ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg
    10441 agagcgaatc aatacttgct caagacgatt gagatgttta atacctgcaa gatgaggatt
    10501 gcgccccagc cgcaccgggc ttagcgccaa cgtcatcccc tcgttacgca aacggtcgta
    10561 atgtgcagga taagccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct
    10621 gtaccctcgc ccgccactac cgcgactgat cacgactttc agtacaccat tttgctgttc
    10681 tgctgcaagc gttttcatct cttgttcaag ctgtggccag aagtcacagg aaatcatcaa
    10741 ccgctgacaa gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc
    10801 gataactctg gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc
    10861 cagcgattcc tgcttataac cgttaattaa gaacataccg gctccttatc agagaattgt
    10921 tagtgtggca gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg
    10981 caagcggacc ttttataagg gtggaaaatg acaacttaga tctttttaaa gatcaaagaa
    11041 ccattagtgc caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga
    11101 cgtgcttcat gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg
    11161 gttggcggaa cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc
    11221 gccgcaccta acagatgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca
    11281 gcttcaccga agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa
    11341 gtaccgtgcg cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca
    11401 tttgccattg ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca
    11461 tcgctgctca taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg
    11521 tgttcgtact cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc
    11581 tctttatccc acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt
    11641 gccgcgccaa aaccaccaac gcccagcggc gtactggctt tctctgcgcc acctgcaacc
    11701 atcacgtcag catcgccata cgcgataata cgcgcagcat gaccaatgtt gtgcacgccg
    11761 gaagtacagg cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga
    11821 tgacctgcca ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga
    11881 ccaccgttca tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag
    11941 ccaattgcgg caccaatgcg ggttgcgttc tcttccgtta tttcaaggcc agaatcctgc
    12001 atggcctgaa cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct
    12061 ttgcgcgaga taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca
    12121 taggcgctag tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct
    12181 ttccaggtag actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact
    12241 acacgacgct tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa
    12301 aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg
    12361 ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc
    12421 tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg
    12481 cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt
    12541 tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca
    12601 aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat
    12661 cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa
    12721 tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt
    12781 gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg
    12841 taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg
    12901 aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt
    12961 gtcatgtccg tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca
    13021 acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg
    13081 gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga
    13141 cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg
    13201 gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg
    13261 ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga
    13321 acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct
    13381 aaataatcac tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg
    13441 ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc
    13501 agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag cgccgctgcc
    13561 atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg
    13621 ccagtaagca ctttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg
    13681 tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg
    13741 atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacagg aactgttggt
    13801 gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc
    13861 gcacagtgag acggtacgct cactggtaac ggcagagcgc gttttgcgcc cgcagcttta
    13921 caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga
    13981 gagttaaagt ttaccggaga aacgacctga ccttctgcag cttcttcaca cgctttcgca
    14041 atagacgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc cggtacggct
    14101 tcttgcataa acttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca
    14161 ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat tgccggtgct
    14221 ttaccgccct gctgctgcca tacgcgatac agcgcaacag aggcagtcaa cagcgcaggc
    14281 tgagtttgcc aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg
    14341 tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc
    14401 gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat
    14461 tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca
    14521 ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct
    14581 tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc
    14641 acgacattgt ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta
    14701 gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc cgccagcgtc
    14761 tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc
    14821 gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt cagcaattca
    14881 ccataactac cgtcggcatg cagatgggtg gaaatgattc ccggctcttc agaggcagcc
    14941 agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc tgttggatcg
    15001 caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc
    15061 acgtattgat cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca
    15121 aatgccgggc aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa
    15181 gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc
    15241 gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc
    15301 gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc
    15361 accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca
    15421 ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac
    15481 tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag
    15541 ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg
    15601 ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc
    15661 atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg
    15721 gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag
    15781 ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac
    15841 aacaccttcc atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc
    15901 tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga tagaagggat
    15961 tgttttaagc accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc
    16021 aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga
    16081 gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg cccctaagtc
    16141 aaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc
    16201 aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc
    16261 ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga
    16321 actcccacga ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg
    16381 cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt
    16441 gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg
    16501 caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag
    16561 ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga
    16621 aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg
    16681 cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct
    16741 acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa
    16801 cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc
    16861 gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt
    16921 ggcttttgcg cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag
    16981 tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt
    17041 gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt
    17101 gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga
    17161 gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt
    17221 aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc
    17281 acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa
    17341 tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc
    17401 atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt
    17461 tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg
    17521 ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc
    17581 tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc
    17641 gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa
    17701 ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc
    17761 aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc
    17821 ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca
    17881 aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg
    17941 ggcatctgca aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg
    18001 agattgataa ttacgtgcgt aaagagcatc ccctgcactg cgcgggtagc tttaagagtg
    18061 aaggatttgg cattacgctg tttgagcgtt tagaggggcg tgatcctaac acgctggttg
    18121 gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg
    18181 gatagttttt tgcgccggat gcggttactt cgcatccggc aatcggtgct ggcggcctga
    18241 tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatctg cttgtattag
    18301 cgcgcgttac gcagcttttg caaacaacgt ttcaaacctt catccatcgg cgcttcgata
    18361 cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga
    18421 cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga
    18481 tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt
    18541 ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag
    18601 cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga
    18661 atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc
    18721 agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac
    18781 gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag acgatgaacc
    18841 agttcaagaa accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt
    18901 aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca
    18961 tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc
    19021 tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt
    19081 ttatattcag gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg
    19141 taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc
    19201 cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg
    19261 ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt
    19321 ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag
    19381 caagaagtga aaaaactgtg agtaaacggg tgataaatgg taaaagtcat cttgctataa
    19441 caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat
    19501 tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat
    19561 tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt
    19621 tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa
    19681 cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga
    19741 ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg
    19801 tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca
    19861 ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg atatcgaaag
    19921 tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc
    19981 gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa
    20041 agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa
    20101 agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa
    20161 caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc
    20221 gaacaacccg cgcgcgggtg gcatttctcg ccgtatcgaa ggcgacgacc gtaccgaatt
    20281 aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc
    20341 tggcgtcggc aaatctgctg aggcgctgca atgggattta agcttccgtc tgaaacactg
    20401 ggaagccatc aaaaaagccg ctgaaagccg cccggccccg ttcctgattc atcaggagag
    20461 caacgtaatc gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat
    20521 cgataacccg aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga
    20581 tttcagcagc aaaatcaaac tatacaccgg cgagatcccg ctgttcagcc actaccagat
    20641 cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccatccg gtggttccat
    20701 tgttatcgac agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg
    20761 cggcggcgat atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc
    20821 tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac
    20881 gcctgtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg
    20941 tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgtcagcg
    21001 ccttagccca tcactgggtg aatccagtca tcacgtttgt ccgcgttgtt ctggtactgg
    21061 caccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtctgatcg aagaagaagc
    21121 gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttacct
    21181 gctgaatgaa aaacgttctg cggtaaatgc cattgaaact cgtcaggacg gtgtgcgctg
    21241 tgtaattgtg ccaaacgatc agatggaaac cccgcactac cacgtgctgc gcgtgcgtaa
    21301 aggggaagaa accccaacct taagctacat gctgccgaag ctgcatgaag aagcgatggc
    21361 gctgccgtct gaagaagagt tcgctgaacg taagcgtccg gaacaacctg cgctggcaac
    21421 ctttgccatg ccggatgtgc cgccagcgcc aacgccagct gaacctgccg cgcctgttgt
    21481 agccccagca ccgaaagctg caccggcaac accagcaact cctgcacaac cagggctgtt
    21541 gagccgcttc ttcggcgcac tgaaagcgct gttcagcggt agtgaagaaa ccaaaccgac
    21601 cgagcaacca gcaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaagcc
    21661 tcgtcagaac aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga
    21721 aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc
    21781 cgagacgcgt gagagccgtc agcaagctga ggtaacggaa aaagcgcgta ccaccgacga
    21841 gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaat gatgataaac gtcaggcgca
    21901 acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga
    21961 acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta
    22021 cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc
    22081 cgaaccaatt gttcaggaag cgccagctcc acgcacagaa ctggtgaaag tcccgctgcc
    22141 agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa
    22201 cggtggtatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg
    22261 tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc
    22321 gtgcgcgtct ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc
    22381 gcaagatgta caggttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt
    22441 gactgaggtc cctgtcgccg ccgctatcga accggttgtt agcgcgccag ttattgaaga
    22501 agtggctgaa gtcgtagaag cccccgttca ggttgccgaa ccgcaaccgg aagtggttga
    22561 aacgacgcat cctgaagtaa ttgccgccgc ggtaactgaa cagccgcagg tgattaccga
    22621 gtctgatgtt gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca
    22681 ggaagagacg gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc
    22741 tgaagttgtt actcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac
    22801 ggtagccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg cgccaatgac
    22861 gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact ggcagcgccc
    22921 tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc atcatgcctc
    22981 tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta atcaagccct
    23041 ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat tcatcaaatg
    23101 gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc ggcgatccca
    23161 gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgagc ttatttgttg
    23221 agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc ctgcaatgcc
    23281 gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac cagatcgctc
    23341 atctgctgcg tttgccctaa agcgcgatcg ctacctaagg aatccagcga ctccagttcg
    23401 ttcagctgcg tgcctaattc cgcgcgaaca gtcagcacat tgttcagcga gtttttcagt
    23461 ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt tatccgcttc gctatccgcg
    23521 accggcgttt tcagggctgc gatggcacta tccagcatgg caaaaagatt ggtttcagaa
    23581 gcgctaccgt ctggttccgc taccgcgttg ctggtaatac tgtcgaaaat tttgtcaccc
    23641 gtgtgcccta tcaccatcga acgcgaagca tcgacctgtt gtttaatact ttctgctcca
    23701 ccgacgtatt tccctttctc ttcgctaaac ggcgcagtct ctgttttata accggcaaaa
    23761 atgtagcgcc cgttaccgtc agtggtgttc gccagattca gcaactggtc acgaagcccc
    23821 tgaatatccg tagccagcga ggcccggtca tcgtcactca aggggccatt gctggcgtag
    23881 acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac actctcttcc
    23941 agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt ttgcgcctgt
    24001 gcctgggaga gaactacggc ttgtgatgct gcaatgggat cgtcagaagg gttaacgact
    24061 cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga attggtgatg
    24121 ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc tccccttagc
    24181 gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca
    24241 ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg acaccggaaa
    24301 tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg ctactggttt
    24361 tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg tcgttaaagg
    24421 atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc tgaccgttgc
    24481 ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct tcgtcggtga
    24541 ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag ctgtcgttaa
    24601 cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta ccgttggcat
    24661 ccggcgtcac cgtaaaagtg gtattgctgg caaggcgggt gacctgccac tgattattat
    24721 cgaacgagat tttgtaatct gtcgccagta ccgcggaggc atcagttacc gtggcaccga
    24781 tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg atagcaaaga
    24841 aatcttcacc agcatcgccg ttggcatcaa atccggcttt gtgttggctg ttgaaagcct
    24901 cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga tcctgagaac
    24961 ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg atctcaatat
    25021 tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg ctggaaggaa
    25081 ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg gccatcgtga
    25141 tgttataagt accgccatcc tgaacgctga cttctacgcc aacaatctgg tttaattcgc
    25201 tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc cccacgcctg
    25261 tcaggcgcga gatttgatcg ttcaggctgg caatttgttt agcgtaattg ttgatctgat
    25321 caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga tattgatcgg
    25381 tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg cgcgctgccg
    25441 ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc atctgtgttg
    25501 ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc tcatagcggg
    25561 cggtcaggcc gctactttgc gtctgcgcag cacgtaactg gttggtaata aacgcatcat
    25621 actcacgctg cacaccagaa acgtagacgc cattgccaac ccagccgcca gcgcccaacg
    25681 tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg ttatagctgg
    25741 agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg ctcatggcgt
    25801 tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag tattatcggc
    25861 agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc tgtaggtttt
    25921 gctcaccttg tcgcttatcg atttcatctg ctggatcatg ttggtgagtt tacgggcata
    25981 gtgaggatcg gtggcatagc ccgcgtcctg tagggcctgc gccccctgtt ccgcactcgc
    26041 ggcggtcgtc acggcggcgt agcgcgggtt acgcgttaac agcccaacgt aatccgacaa
    26101 ggcttccaga tacgagctgt agacgcgaaa cttcgctttt actttcttcg cttcgccgtt
    26161 ttcatattca gtcgtggtga tttcagtaac tggccctttc cagttgccag aggctttgac
    26221 accaaacagg ttatagctcg gctcgccgtt ttcgcggcgg atttgccgtt gcccccaacc
    26281 agattccagt gccgcctgag cgaggatcaa atgatgtggc acaccgcttt gctggcttgc
    26341 cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta ctgtcacccg gcagcgaatc
    26401 atcgtagtta cgtggcacgg ccttttgcac cagctgcgaa agcgcctgat tttgataacg
    26461 caccacggtt tcgagcggga atttcatcgg tgctgctggc gtggactcct ctggcaatgg
    26521 ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt tgcccgccgt
    26581 catctgttgg gcaatctgct ggtcatacat actggtatac agacgagtgt gctcgctgct
    26641 gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct gcacgaacat
    26701 cccttccacc tggcgggcca ccggacggat atttgccgcc ggatcttcgc ccgctttcgc
    26761 ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt tgctgtcgct
    26821 gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat tgattgcaaa
    26881 atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcgcgcac cacgttattg
    26941 aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa atcgatctgc
    27001 gtttgtggag taaccacagt ctgtccacca ccaaacggtg tatctggctg gctgacattg
    27061 gcctgacgat tgactgttac tgagagattt ccctgcgcta ccgcgcagct gtcgagggtc
    27121 acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt agcgtcctgc
    27181 ggggtgacat taacctgcat attctggata tctgcaagga aacggacctg ggaactgttg
    27241 ccacttggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct gccatatcca
    27301 cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc gtcgttaagt
    27361 tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc aataaccgca
    27421 ccattggtga tccgtccacc gttcagttgg ttcacctgaa cactgctacc gccagcggag
    27481 gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg actgtcaacg
    27541 cccttaagcg gcgtcatcaa caacgtacct ccacgcaagc ttttggcatt tcccatggaa
    27601 gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga cgctgtcacc
    27661 attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt aattcccagc
    27721 tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt ctggtcaccg
    27781 gtgccatcca gccccaccac cagaccatag ccaatcagtg agttttgcct taccccctga
    27841 acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag tagaagaatt
    27901 aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg ttaaggaaga
    27961 aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct acgtattcaa
    28021 tgcgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata gtgcgtggat
    28081 taaccacgcc agagaagcga ataaattcgg taccctgatt aatggcaatc tgtttttcac
    28141 ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc aacgtgccgc
    28201 taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca ccggaggctt
    28261 cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca gtatcaaagc
    28321 caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg gcgctgacgt
    28381 tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga tcttcaaaca
    28441 gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg ttggcgacgg
    28501 gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc gtggagggta
    28561 tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa gtatgcgcag
    28621 cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct gccaccggtt
    28681 aagccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac cgctttactg
    28741 ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc cacgttgacg
    28801 ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc cggcgtgctt
    28861 tcgttcggtg caccagagga ttgcgtttcg gtgtagaggt tttcgccaat gctctccagc
    28921 ccggtgtcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg agctgcctgg
    28981 ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa cgcattcgcc
    29041 ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg cccgttctga
    29101 tccacctgga aagagccgtc acgggtatag gctgatgaac catctggcaa catcacctgg
    29161 aaaaagccct gccctttaat cgcgacatct ttgctgttgt tggtctgcga caggtttccc
    29221 tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg taatccggag
    29281 ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg ataaagcaga
    29341 tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc caggttgttg
    29401 gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat ccataatgaa
    29461 ctgatcataa aatgtcctgt agtggattag tgacaagcag agcagaagca cgcgacagtg
    29521 tgaatttccc ctcaccctaa ccctctcccc aaaggggcga gggaatagtc cgcgcgcttt
    29581 tattgcaatg tcatcaatcg ttcaattaac tcatcgacag cagttggttg gcacggcctg
    29641 cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc gcgctggcaa
    29701 tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc cccgacatca
    29761 cacgcaaggt tggatctgcc tgcagtaccg gcccacgcgt ggcctgggtt tctgcgctta
    29821 aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc accagtttca
    29881 gacgccccac tggcgcaacc gtatttgccg gatcgcccgg attgagcgcc gagattgtgc
    29941 catcggcagc aatagtgatt tccgcccctt ccggcacagc aattggcccc gcctcgccta
    30001 tcaccggatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg ctgccattac
    30061 gcgtataccc ttcgctgccg tcagcggtct gcacggccag ccagccatcc tgctgcaacg
    30121 caacgtccag cgggcgcgag gtgtaatcca tcttgccggg cgtcatgtct gcacccggtg
    30181 ttgacgccgt taccagcgtg cgcgtaggca gtgaaagccc ttccaccggc actgcacgca
    30241 aggcgtttaa ctgtgcgcga aaaccgggcg ttgaggcatt ggccagatta ctggcggtta
    30301 ccgcctgttg attcagcgtc tggctggctg ctcccatcgc ggtataaatt gcgtgatcca
    30361 ttgagctatc ccgtcagcga ttagcgtaag ttaaccagcg tgttgaggat ctggtcctgg
    30421 gttttgatgg tctgggcgtt agactgatag ttacgctggg caacgatcat attgaccagt
    30481 tctttactga gatcgacgtt ggacgcttcc aacgcaccgt tggtcagggt gccaaagttg
    30541 cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga ccagacgttg
    30601 tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat ctgccccagc
    30661 agttgtgttt gttcgttgga atagttgccg acaaccgtac cgtcatcatt gatttgataa
    30721 ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatatt gttagcgccg
    30781 gtattttgct gcatggagtt gaggaagctc aggctaaacg tggcgggatc tgcgccgtta
    30841 attgcgccag tggtaatatt ctctgttgga tttgaggtca gaacgccatt ggcattaaac
    30901 accagcttca ttgcaggctc ggctgtacct gttggatcac tgctatcctg ggtgtagacc
    30961 tgccagttat tatccccggt cttcacaaag tacacgctca tgtcatgagc attaccctga
    31021 ctatcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct ggcgctgaat
    31081 ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga tgccgtggtg
    31141 gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt cgccccttgc
    31201 tgaatagtcg gcggcgtacc ggttgccggg taacccgtca gctgtaaacc ttgcatattc
    31261 accaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa caccgaaccg
    31321 ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag acctcgcccg
    31381 gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt tacccccagt
    31441 cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt aaagccgtag
    31501 gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc agcgtttaat
    31561 ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc ttaaattatc
    31621 tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt attaccgctg
    31681 ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac cagttgtgta
    31741 ccaccgttac tggcgctaat cgctacattg taagaaccgt tcggcgcagt gctgccgtca
    31801 gtcaacgtac cgtcccaggt gaaactgtga actccggcgg tcagttcacc aatatcaatg
    31861 gtgcgcacaa ccgcaccatt tttatcggtg atggtggccg taactttgtc tgccgcctgt
    31921 tgcagctcaa caccaaacgg cgtggtcgtg gtcacagctc cttcttcact gccggttccc
    31981 gcaagaacag tggtgccggg gatcatcacg ccgtgaccga tcaggttact ggcctgtaac
    32041 gactggctgt tatcaatctg tccggaaata gatccgagtg tggtattgag tttttcaatc
    32101 ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc cattggattg
    32161 gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca gaaaactgct ttgtaaatct
    32221 gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt atttgtcgga
    32281 tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc gtaagggttt
    32341 tcagcatcat gcttttcacc gtgttgagca cttcaacgtt ggcctgatag ctgcgtgacg
    32401 ccgacatggt gttaaccatc tccccgacga catcaacgtt cggcattttt acgtagccct
    32461 ttgcatctgc cagcggatta cccggttcat aaaccagttt gtccggggcc tgactttcta
    32521 taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata
    32581 ccacctgttt tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca
    32641 gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat
    32701 caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt
    32761 tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact
    32821 gggtgcgttc gcgatccata tcgacggtat taccgtcaag cgaaggctgg tccggaatac
    32881 ggtattgcag ttctgcggta ggaggcctca gcgcctgcgc cgggatgtgt tgcgttgagg
    32941 tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg ttgcatgact tttttaagtt
    33001 cactggcaaa atcgatatcg cgcgcctgat aaccaggggt atcggcattg gcgatgtttg
    33061 ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg
    33121 cggcgtcgag cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta
    33181 aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg
    33241 ttgatggtca ttgcggacag gtacaattca cgttgtagaa atggctgggg gcgaaaatgc
    33301 tgacaataaa acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtacggcga
    33361 gcaatctcac atcgcaattg cacaactttt ttagcgccca gctcgcgggg gtaagtgatg
    33421 aggttcgtgt ttctattcgt acagcgccca atctactacc gccgtgcgag cagccattgc
    33481 tttcgatgag caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta
    33541 acgacaaacg atatttacag gttaatgtac aggccacagg aaattatgtg gttgccgcga
    33601 tgcccattgc gcggggagga aagctggaag ctggcaatgt caaactgaaa cgcggacggc
    33661 tggataccct gccaccgcgt acggtgctgg atatcaatca acttgttgat gccattagcc
    33721 tgcgcgatct atcacccgat caaccaatcc agttaaccca gtttcgccag gcatggcggg
    33781 taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc gccaacgcag
    33841 aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg
    33901 gacaggtagt cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg
    33961 ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg
    34021 aggaataaac catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac
    34081 cgcgcgaaac cactgacgcg ccggtaacga acacccgggc ggcaaaaaca acagcctcca
    34141 ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg
    34201 atatcaatct tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa
    34261 tggacaccgg caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta
    34321 actgatcgta tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat
    34381 ctcaaaacgg taatggatca agaacagcaa catctctcta tggggcagat caacggcagc
    34441 cagttgcaat ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa
    34501 cagttacgca agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag
    34561 gaaattactg tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg
    34621 gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg
    34681 acgctatatg gggcgaacgg tcagacctca acaacccatc gcggcggtaa aaagatttcg
    34741 atctgaatat tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg
    34801 ggcaaattct ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc
    34861 cgccagcacg accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc
    34921 cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag
    34981 acgcaacaga aacgccatcc agccgggttg cggggtaaag attttctgct tacgcaactg
    35041 ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc
    35101 atgtttcaac ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat
    35161 ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag ccaaaacttt
    35221 cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag
    35281 cgcatcaaag gcggtaaatt taccgtactg gaacagcgaa acggtcaacg gacctgaaag
    35341 aatgcccaac gcaaccgcac tcggcagcgc caacaggaaa caaagacgca gcccccagtc
    35401 catcaaacgg ttgtattcat catgattgcc actggcaaaa ctcttcgaca gcgacggcag
    35461 caaaatggtg ccaagcgcca cgcccagcac accagacgga aactccatta agcggtcggc
    35521 gtaatacatc caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat
    35581 taaggagatc tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg
    35641 cattgctccg gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag
    35701 gtgcggtagc tgatacacca gctgcaggat gccgccgacc gtaacagccc acgccagcgc
    35761 cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca t
//
LOCUS       DAFATA010000038        35571 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFATA010000038 DAFATA010000000
VERSION     DAFATA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.038"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..260
                     /locus_tag="LGJ20_002281"
     CDS             <1..260
                     /locus_tag="LGJ20_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="LptA; periplasmic binding protein part of a
                     putative ABC superfamily of uptake transporters; interacts
                     with LptB protein, the ATP binding component of the uptake
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBK2386335.1"
                     /translation="NGKPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYL
                     VKEQKMQAFSDKGKRVTTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            267..992
                     /gene="lptB"
                     /locus_tag="LGJ20_002282"
     CDS             267..992
                     /gene="lptB"
                     /locus_tag="LGJ20_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBK2386336.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            1040..2473
                     /gene="rpoN"
                     /locus_tag="LGJ20_002283"
     CDS             1040..2473
                     /gene="rpoN"
                     /locus_tag="LGJ20_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK2386337.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEINTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            2496..2783
                     /gene="hpf"
                     /locus_tag="LGJ20_002284"
     CDS             2496..2783
                     /gene="hpf"
                     /locus_tag="LGJ20_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBK2386338.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            2901..3392
                     /gene="ptsN"
                     /locus_tag="LGJ20_002285"
     CDS             2901..3392
                     /gene="ptsN"
                     /locus_tag="LGJ20_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBK2386339.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            3438..4292
                     /gene="rapZ"
                     /locus_tag="LGJ20_002286"
     CDS             3438..4292
                     /gene="rapZ"
                     /locus_tag="LGJ20_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK2386340.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            4289..4561
                     /gene="npr"
                     /locus_tag="LGJ20_002287"
     CDS             4289..4561
                     /gene="npr"
                     /locus_tag="LGJ20_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBK2386341.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            4774..5406
                     /gene="yrbL"
                     /locus_tag="LGJ20_002288"
     CDS             4774..5406
                     /gene="yrbL"
                     /locus_tag="LGJ20_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBK2386342.1"
                     /translation="MIRLSEQNPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(5403..6131)
                     /gene="mtgA"
                     /locus_tag="LGJ20_002289"
     CDS             complement(5403..6131)
                     /gene="mtgA"
                     /locus_tag="LGJ20_002289"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBK2386343.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(6128..6781)
                     /gene="elbB"
                     /locus_tag="LGJ20_002290"
     CDS             complement(6128..6781)
                     /gene="elbB"
                     /locus_tag="LGJ20_002290"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBK2386344.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(7011..9347)
                     /gene="arcB"
                     /locus_tag="LGJ20_002291"
     CDS             complement(7011..9347)
                     /gene="arcB"
                     /locus_tag="LGJ20_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBK2386345.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(9443..10372)
                     /locus_tag="LGJ20_002292"
     CDS             complement(9443..10372)
                     /locus_tag="LGJ20_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK2386346.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            11047..15507
                     /gene="gltB"
                     /locus_tag="LGJ20_002293"
     CDS             11047..15507
                     /gene="gltB"
                     /locus_tag="LGJ20_002293"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBK2386347.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            15520..16938
                     /gene="gltD"
                     /locus_tag="LGJ20_002294"
     CDS             15520..16938
                     /gene="gltD"
                     /locus_tag="LGJ20_002294"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBK2386348.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            17122..18249
                     /locus_tag="LGJ20_002295"
     CDS             17122..18249
                     /locus_tag="LGJ20_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBK2386349.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERILLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(18309..18773)
                     /locus_tag="LGJ20_002296"
     CDS             complement(18309..18773)
                     /locus_tag="LGJ20_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBK2386350.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(18770..19645)
                     /gene="nanK"
                     /locus_tag="LGJ20_002297"
     CDS             complement(18770..19645)
                     /gene="nanK"
                     /locus_tag="LGJ20_002297"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBK2386351.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(19642..20331)
                     /locus_tag="LGJ20_002298"
     CDS             complement(19642..20331)
                     /locus_tag="LGJ20_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK2386352.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(20379..21869)
                     /gene="nanT"
                     /locus_tag="LGJ20_002299"
     CDS             complement(20379..21869)
                     /gene="nanT"
                     /locus_tag="LGJ20_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBK2386353.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSPKNDLVKTKS"
     gene            complement(21978..22871)
                     /gene="nanA"
                     /locus_tag="LGJ20_002300"
     CDS             complement(21978..22871)
                     /gene="nanA"
                     /locus_tag="LGJ20_002300"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBK2386354.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGQIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(22993..23784)
                     /gene="nanR"
                     /locus_tag="LGJ20_002301"
     CDS             complement(22993..23784)
                     /gene="nanR"
                     /locus_tag="LGJ20_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBK2386355.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            24164..25531
                     /gene="dcuC"
                     /locus_tag="LGJ20_002302"
     CDS             24164..25531
                     /gene="dcuC"
                     /locus_tag="LGJ20_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBK2386356.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVLLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(25574..26071)
                     /gene="sspB"
                     /locus_tag="LGJ20_002303"
     CDS             complement(25574..26071)
                     /gene="sspB"
                     /locus_tag="LGJ20_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBK2386357.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDELPQPPRGGRPA
                     LRVVK"
     gene            complement(26077..26715)
                     /gene="sspA"
                     /locus_tag="LGJ20_002304"
     CDS             complement(26077..26715)
                     /gene="sspA"
                     /locus_tag="LGJ20_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBK2386358.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(27110..27502)
                     /gene="rpsI"
                     /locus_tag="LGJ20_002305"
     CDS             complement(27110..27502)
                     /gene="rpsI"
                     /locus_tag="LGJ20_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK2386359.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(27518..27946)
                     /gene="rplM"
                     /locus_tag="LGJ20_002306"
     CDS             complement(27518..27946)
                     /gene="rplM"
                     /locus_tag="LGJ20_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK2386360.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(28165..29292)
                     /gene="zapE"
                     /locus_tag="LGJ20_002307"
     CDS             complement(28165..29292)
                     /gene="zapE"
                     /locus_tag="LGJ20_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBK2386361.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            29486..29884
                     /locus_tag="LGJ20_002308"
     CDS             29486..29884
                     /locus_tag="LGJ20_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBK2386362.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            30038..31405
                     /gene="degQ"
                     /locus_tag="LGJ20_002309"
     CDS             30038..31405
                     /gene="degQ"
                     /locus_tag="LGJ20_002309"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBK2386363.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            31495..32562
                     /gene="degS"
                     /locus_tag="LGJ20_002310"
     CDS             31495..32562
                     /gene="degS"
                     /locus_tag="LGJ20_002310"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBK2386364.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(32624..33562)
                     /gene="mdh"
                     /locus_tag="LGJ20_002311"
     CDS             complement(32624..33562)
                     /gene="mdh"
                     /locus_tag="LGJ20_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBK2386365.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            33997..34467
                     /gene="argR"
                     /locus_tag="LGJ20_002312"
     CDS             33997..34467
                     /gene="argR"
                     /locus_tag="LGJ20_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBK2386366.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            34832..35095
                     /gene="yhcN"
                     /locus_tag="LGJ20_002313"
     CDS             34832..35095
                     /gene="yhcN"
                     /locus_tag="LGJ20_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBK2386367.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(35151..35423)
                     /locus_tag="LGJ20_002314"
     CDS             complement(35151..35423)
                     /locus_tag="LGJ20_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBK2386368.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
BASE COUNT         8786 a         9423 c         8975 g         8387 t
ORIGIN      
        1 acaacggtaa accggttgaa ggtcacgctt cccagatgca ctacgaactg gcaaaagatt
       61 ttgtcgttct gacgggtaat gcttatctgc agcaggttga tagcaacatt aagggcgata
      121 agatcactta cctggtaaaa gagcagaaaa tgcaggcttt cagcgacaaa ggcaagcgcg
      181 taaccaccgt tctggtgccg tcgcagctgc aggacaaaaa caacaaaggc cagaccccgg
      241 cacagaagaa gggtaattaa ttcgttatgg caacattaac tgcaaagaac cttgcaaaag
      301 cctataaagg ccgtcgcgtg gtagaagacg tcagcctgac cgtcaactcc ggggaaattg
      361 tcggtctgct ggggccaaac ggtgccggta agaccaccac tttctacatg gttgtaggca
      421 ttgtgccgcg cgatgcgggc aacatcatta ttgatgatga cgatatcagt ctgctgcctc
      481 tgcatgcacg cgcgcgccgc ggtatcggct atctgccaca ggaagcctcc attttccgtc
      541 gcctcagcgt ttacgataac ctgatggcgg tactgcaaat tcgtgacgac ttgtctgctg
      601 aacaacgtga agaccgcgcg aacgagctga tggaagagtt tcacattgag cacctgcgtg
      661 acagcatggg gcagtcactc tccgggggtg aacgtcgccg tgtagaaatt gcccgcgcac
      721 tggctgcgaa tccgaaattt attctgctcg acgaaccgtt tgccggggtt gacccgatct
      781 cggttatcga cattaaacgc atcattgagc acctgcgcga cagcggcctg ggcgtgctga
      841 tcactgacca caacgtgcgt gaaacactgg cggtttgtga acgcgcttat atcgtcagtc
      901 aggggcattt gatcgcccac ggcacgccta cagaaatctt acaagacgaa cacgttaagc
      961 gtgtatacct tggggaagac ttcagactct gatagggtag aagtttgcga cgttttagca
     1021 ggagagtacg attctgaaca tgaagcaagg tttgcaactc aggcttagcc aacaactggc
     1081 gatgacgcca cagctccaac aggcaattcg tctgttgcag ttgtcgacgc tggaacttca
     1141 gcaggagcta cagcaggcgc tggagagtaa tccgctgctt gagcaaatcg acactcatga
     1201 agaaatcaac acccgcgaaa cgcaagacag tgaaacgctg gacaccgccg acgcgctcga
     1261 acaaaaagag atgccggaag agctgccgct cgatgccagt tgggacacca tttacaccgc
     1321 tggtacacca tccggcacca gcggtgacta cattgacgat gagctgccgg tctatcaggg
     1381 cgaaacgacg cagaccttgc aggattacct gatgtggcag gtcgagctga caccgttttc
     1441 cgacactgac cgcgctattg ctacctctat cgtcgatgcc gttgatgaca ccggttatct
     1501 gactgtcccg ctggaagata ttctcgaaag tatgggcgat gaagagatcg acatcgacga
     1561 ggttgaagcc gtccttaagc ggatccaacg gtttgatccg gtcggtgtag cggcaaaaga
     1621 tctgcgtgac tgcctgctaa tccaactctc ccaattcgat aaaaccacgc cgtggctgga
     1681 agaggccaga ctgatcatta gcgatcatct cgatctgtta gccaatcacg acttccgcac
     1741 tttaatgcgc gtcacgcgtc tgaaagaaga tgtgctgaaa gaagccgtca atctgatcca
     1801 gtcgctcgat ccgcgccccg ggcagtcgat ccagactggc gaacctgagt atgtcattcc
     1861 agatgtgctg gtgcgtaagc ataacggtca ctggacggta gaactcaaca gtgacagcat
     1921 tccgcgtctg caaatcaacc agcactacgc ctcgatgtgc aataacgcgc gcaacgatgg
     1981 tgacagccag tttatccgca gcaatctgca ggatgccaaa tggttgatca agagtctgga
     2041 aagccgtaac gatacgctac tgcgcgtgag tcgctgtatc gttgaacagc agcaagcctt
     2101 ctttgagcaa ggtgaagaat atatgaaacc gatggtactg gccgatatcg cccaggctgt
     2161 cgaaatgcat gaatcgacga tatctcgcgt gaccacgcaa aaatacctgc atagtccacg
     2221 aggcattttt gaactgaagt atttcttttc cagtcacgtc aataccgagg gcggcggcga
     2281 agcttcctcc acggcgattc gtgcgctggt gaagaaatta atcgcggcgg aaaacccagc
     2341 gaaaccgttg agcgacagca agttaacctc tttgctgtcg gaacaaggta tcatggtggc
     2401 acgccgcact gttgcgaagt accgagagtc tttatccatt ccgccgtcaa accagcgtaa
     2461 acagctcgtt tgacccaacc gataaggaag acactatgca gctcaacatt accggaaata
     2521 acgtcgagat caccgaggca ctgcgcgaat ttgttacagc caaatttgcc aaacttgagc
     2581 aatattttga ccgaatcaac caggtctatg ttgttctgaa agtggagaaa gtcacccaca
     2641 cctcagatgc aacactgcat gtaaacggtg gtgaaattca tgccagcgcg gaaggtcagg
     2701 acatgtacgc tgccattgat ggcttaattg ataagctggc acgccagttg accaaacata
     2761 aagataaact gaaacaacac taattgtccg ggcaattagc atgtgcatgg cggcctgttg
     2821 tgcggcacaa cgggccattt gtacggttaa tgctccgagc ctgttccact gtttgagtgg
     2881 gcaggttctt aggtgaaatt atgacaaata atgatacaac tctacagctt agcagtgttc
     2941 ttaacaggga atgtacgcga agccgcgtcc actgtcagag caaaaaacgc gccctggaaa
     3001 tcatcagcga gctggcggcg aaacaactta gtctgccgcc tcaggtggtt tttgaagcta
     3061 tcctgacgcg ggaaaaaatg ggcagtaccg gtatcggcaa tggtattgcc attccgcatg
     3121 gcaaactgga agaagatact ctgcgcgccg ttggcgtttt cgttcagctc gaaacgccta
     3181 tagctttcga tgccatcgac aaccaaccgg tggatttact ttttgccctg ctggtgccgg
     3241 cagaccaaac taaaacgcac ctgcatactc tgtcactggt ggcgaaacgt ctggcggaca
     3301 aaaccatctg ccgccgttta cgcgcagccc agagcgatga agagctgtat caaatcatta
     3361 cggataccga aggtactccg gatgaagcgt agttattcgg taatgtctct tttagacgtt
     3421 gtgaggagaa acagtacatg gtactgatga tcgtcagcgg acgttcaggt tcaggtaaat
     3481 ctgtcgccct gcgtgcgctg gaagatatgg gtttttactg cgtggataac cttcccgtag
     3541 tgttgttacc cgatctggct cgaactctgg ccgatcgcga gatttctgcc gccgtcagca
     3601 ttgatgttcg taatatgccg gagtcaccag aaatattcga acaggcgatg agtaacctgc
     3661 ctgacgcttt ctcaccgcaa ctcctgttcc tggatgccga ccgtaatacc ttaattcgtc
     3721 gttacagtga cacgcgccga ctgcatccgc tttccagcaa aaacctgtcg ctggaaagtg
     3781 ctatcgacaa agaaagcgat ttgctggaac ctctgcgttc gcgagcggat ctgattgtcg
     3841 atacctcaga aatgtccgtt cacgagctgg ctgaaatgct gcgtacccgt ctgctgggta
     3901 aacgtgaacg cgaactgaca atggtctttg agtctttcgg cttcaaacac ggtatcccta
     3961 tcgatgcaga ttacgtcttt gacgtgcgct tcttgccgaa cccgcactgg gatccgaaac
     4021 tgcgtccaat gacaggtctt gataagcccg tcgccgcgtt cctcgaccgc cacacagaag
     4081 tacacaattt tatctaccag acgcgaagct atcttgagct atggttacct atgctggaaa
     4141 ccaacaaccg tagctacctg acggtcgcca ttggttgtac cggcgggaag caccgttcgg
     4201 tgtatattgc agagcaactg gcagactact tccgctcgcg cggtaaaaac gtccagtcac
     4261 gccatcgtac gctggaaaaa cgtaaaccat gaccgtcaag caaactgttg aaatcacaaa
     4321 caagctgggc atgcatgccc ggcctgcaat gaagctgttt gaattaatgc agggttttga
     4381 cgctgaagtg ctgttacgta atgacgaagg caccgaggct gaagccaata gcgttattgc
     4441 actgctgatg ctggattctg ccaaaggacg gcagattgaa gttgaagcca ccggtccaca
     4501 ggaagaggaa gcactggccg ccgttatcgc cctctttaat tctggttttg atgaagatta
     4561 atcttcatca ctttgacatt caaatccctt caagctcccc cgacaataag aaaagcgcgt
     4621 actgaaatcg ttctcaatca acgtcatttg tacattttgt gcgcttttca ctttcagaag
     4681 aaccttaaga aaaccttaag aggcattgtt taggttttgt ttaagttaat cgaccatact
     4741 ggagatcgtc agaaaatatt tccaggagat ggcatgattc gcttatctga acaaaatccc
     4801 ctgggcaccg gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc
     4861 aagattgtct accatcgtgg cgatggtggc gataaagaga tccgccgtga gttaaagtac
     4921 tacgcgcatc ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc
     4981 gttgagacgg attgcggaac cggttatgtc tacgatgtga ttgcagattt tgacggcaaa
     5041 ccctccatta cgctgaccga atttgctgag caatgccgtt acgaagaaga cattgcacaa
     5101 ctgcgccagc tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg
     5161 tcgctgaagc cgcagaatat cctctgtcac cgcatcagtg agtcagaggt tatcccggtc
     5221 gtctgcgaca acattggtga aagcacattg attccactgg cgacctggtc gaagtggtgt
     5281 tgtctgcgta agcaagagcg gttatggaaa cgatttatcg cccaaccggc gctggctatt
     5341 gccctgcaga aagatctgca acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa
     5401 gcttaatcca gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt
     5461 aaaatccacg cctgacggct acgcacgtag cccgatggcg aggagacttt gaaacgaagt
     5521 ggattaggta atacagctgc cagtaatgca gcttccgacc gggtaagttt gctcgcgggt
     5581 ttgtggaaat aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg
     5641 atattcaggt aaaccgtcag gatacgcttt ttgctccaga ctgtttctat ccccagcgtt
     5701 aatccagctt ccagcccttt tcgtacccag ctacgcccat cccataaaaa gagatttttg
     5761 gccgtctgct gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg ctcgttgtgc
     5821 gccagggctt tctcaatgga agcgacatca aagccccagt gctcaggaaa tttctgatct
     5881 tctgcggcaa tcaccgccag tcccatccac ggcgagattt gatccatact gacccagtca
     5941 gaatgtgcca cgtaacgaaa attgccatgc agccaggcgc tgacctgtcg ctcgaccatt
     6001 accgctgaga agggaacagg cgcaacacta aacaacgcga tgcccccgcc ccagaaaacg
     6061 gcgaggacaa ccattaaccg caaaagaaaa cggcgaacga aactaaacac cgttaagcgg
     6121 cttttactca ttcagccaga accagcacgc gggaaaccag cttatcaatg ccgctcgccg
     6181 cttctgcaat gttctgcgcc agcatatatg ctggggtggt gacaatctta ttgtcttcat
     6241 caaccacgat atcatcgaca ggacagggca catgctccgc gcccatctct tccagcactt
     6301 ctgcggtatc gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa attttcggca
     6361 gcatcgctgg ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg
     6421 ccagcgcctt taattcacgg tcaacggtgc attcgctacc aagagtggca aaattgctta
     6481 aattcttcgc cgcgccaaac ccccccggca caatcaacgc atccagttca gcggcatcgg
     6541 cctgggccag aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc agcacgttgc
     6601 gcgtttccgt catcgcttcg ccagttaaat ggttgataac atcaacctgc tgcttatccg
     6661 gtgcaaagca gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt
     6721 catgaatttc agaaccgtca tagacgccgc atccgctcag aattacgcca attttcttca
     6781 ttgtgatcat cctttcgcaa ttgactgaaa cacattaatt tttttaataa aaatggtacg
     6841 catcacacat ttaactgatt catgtaacaa atcatttaag ttttgctatc ttaactgcgt
     6901 gcggcctgaa aaacagtgct gtgcccttgt aactcatcat aataatttac ggcgcagcca
     6961 agatttccct ggtgttggcg cagtattcgc gcaccccggt ctagccgggg tcatttttta
     7021 gtggcttttg ccacccacgc tttcagcact tctacgtcgt gacgccactc ttctttcatc
     7081 tcttcaatcc attcaccgac gttatcttcc caggcaggaa ggtccggaga ctgaatttgc
     7141 tgacccagct gttgcagatg gcgtaacccc actgaccccg ccgcaccttt aattttatgt
     7201 ccttcctcta caatgccttt tttatcctgc gccgtcagat tcgactccag cacgctgaca
     7261 tagcccggca tcatcttctc aaacaccgct aacccgtcgg tgatcagctt cggtcctaca
     7321 agttcgagat actgttccag catgggaata tcgagcaatg cttctgattt actgttctct
     7381 tctgtcgtca ccgtactctc ctcatcatcc tgggtatccc agaatttctt gatcatcgcg
     7441 gttagcgccg gaaccgaaag cggcttactc agcacatcat ccattccagc attgaggtac
     7501 tcttgtttgt ctttcagcac gttagcggtt aaggccacca gcggcggtaa atcctcgcgc
     7561 ggataacgct tcgtcagttc acgagagata tccagcccgg tcatatctgg caactgaata
     7621 tccagcaaca ccaggtcgta ttcgcccggt ttaaacatct ccagcgccgc cttgccggtc
     7681 atggcgacat caacgctgtt acctaatttt tccagcacag aacgcgcaac aatcacgttc
     7741 agttcaatgt cttccaccag cagcacattc agcgccggta aaggcatatc gtcttcatca
     7801 aacgcatcat cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa
     7861 cctttgccct gttcgctggt aaccgtaata tcgccgccca tatttttcgc cagacgacga
     7921 gaaacggcca gaccaatacc ggtgccggtg gcaggtttac cgccatgact gtctttcacc
     7981 tggtaataca tggcgaaaat tttatccagc tcatcctgcg gaatgccgat accagagtct
     8041 tccacttcaa aatgcagcat atcgccttca tcgtagcgca cgcgcacggt aacctggcct
     8101 tgctgggtga atttgacggc gttactgatg aggttccaca ggatctgccg taaacgcgtc
     8161 ccgtcggtaa tgacctgatg cggtaatggc agcgtcggct ccaggttaaa gcgcaatcct
     8221 ttttgttgcg cctgcaatgc ggagagattt tccagatcgg caaggaagct ggtgaaatca
     8281 accggttgat tatcaagctg gaccttgcgc cgttccatct tatccatgtc gataatatcg
     8341 ttaaagatat tccccagcgt gacggccgaa acatggatgg tcttgagata tttttcctgc
     8401 tcggcggtga gttcggtatc cagcagaata cggctcaggc cgacgatacc gttcagcggt
     8461 gtacgcaatt cgtgactgat ggtggagata aacgtcgttt tgtcgcggct ggcccgttca
     8521 agcgcatcct gataccgctt acgctcggta atgtcgcgac caaagcccat caaaccgtga
     8581 cgtttaccca cgcggtcgta gtacggcact ttacggattt caaagcaggc tttgcgcccg
     8641 tccgggtaat ccagccactg ttcataggtc agtgacacat tatgacggaa cactttttca
     8701 tcggtttcaa tgacttttgc ggcggcttcc ggtgagtaaa catcagcagg tttcaggtga
     8761 accagttgtt tttcgctttt tccggtcagc agctccatcg cgcggttaca gccggaaaac
     8821 tctttatctt cgttacgata aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaag
     8881 aatgaggatt gctgctcgag ctgaatttgt gtctcttcgc gctctttgat ttcaattttc
     8941 agttggccga aggtttcctg cagttctgct tccgcttttt cacgaacggc gatttcctga
     9001 tttagctggg caatattatc ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc
     9061 tcctccagtt tttgcaccag ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg
     9121 accaccgata gaaagtagac cgcccacggc gtaatcagca aaccaaagaa gatagaacga
     9181 ataacatcaa tgctttcgac ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca
     9241 atggcaagaa cgacgagggc cagcgccagc aacattgaga agcgcaccag acctaacttc
     9301 atcatcaggt caacataata ctgcgccagc agacgaattt gcttcattag ggaattcctt
     9361 cacgacaacc tgccccaata atacccaatt ctgacagtta cgttataaat tgtgtgagaa
     9421 atgcggaatt cccctcaacg acctactccg ttggaggtat ccacggacgt tccagcgccg
     9481 atccctgtac gccatgttca ttcagatagc gatccagctc gaccattccc gtccatcgat
     9541 tttcgcacca cagaggagca agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt
     9601 agatcacttc cggtggcgta tggcgaatca tttctccggc agtgagcgtg taatcctcca
     9661 gttcaatacc gttcaaacgc cccgcttccc aggctttcgc cataatgctg cctttcacaa
     9721 tatgcagcgg atgcagcttt atgccgtcca cgccggtttc aaccactcgc tccagcgttt
     9781 gcaaacattc agcctgccct tcgccaggca ggccgacaat taagtgggaa cacaccttca
     9841 gcccgcgctg acgtgccagc tgggttgtac gctgataaca ggcaaaatca tggccgcggt
     9901 tgatgcgatg cagtgttttg tcgtgggcgg tttgtagccc cagctccagc cacacttcgt
     9961 aaccctggtc cttatattcg caaagcagat ccagcaccgc atccggcacg cagtccgggc
    10021 gggtaccaac acacaaaccg acaatattgg cctggctcac cgcctgctga tacatagaac
    10081 gcagcacctg aacttccgca aaggtgctgg tatacgcctg aaagtaggcc agatagcgtt
    10141 tagcgcggtt aactaaattc gcctggtgcg ccagttgctc ggcaatggaa cgatgctgct
    10201 gcgcttcatc ggcaaacgag gcaacattac agaatgtgca gccaccacgc ccgatggtac
    10261 cgtcacggtt agggcagcta aaaccgccgt gtagcgtcag cttatgcacc ttttgcccat
    10321 aacgacgggt gagatcacca ccaaacatat tgactaattt ctgtaactgc ataatctgat
    10381 agaccgcgcc ttgaaaagag gccaaagcct gccattttta gctcaattcg gcgatgacct
    10441 ggatcaatcg tcccggcctg cttttatcaa ctgcataatc aatcaaaatt accgaaattt
    10501 catgcataat cacataaatc acttttgctt atcttgtgtc agattttttt atctccttat
    10561 ggattttagg caaaaacagt agcatgaaac gtcattacca attaaggcag tataaaatgc
    10621 tggttttctc gtcagttcaa ggcaggataa gggttaacac gcctttatga cagtcaggaa
    10681 ttgactgttt ctctaacgac ttccctttta gccttaaaga taaaatccat tttaatttca
    10741 gtaatttaat aaagaatttt gcgctaaagc acatttctgt accaataagc ttgccatttg
    10801 acctgtatca gctttcccga taagttggaa atccgctgga agctttctgg atgagcagcc
    10861 tgctcatcat atttatgcag taattgagat cccctcttca ccgtattaac cgatgcgaaa
    10921 aggacaacaa gggggcgaat gcgaggcgcg cgtatgacac gcaaaccccg tcgccacgct
    10981 ctttctgtgc ccgtgcgcag cggttcggaa gtggggttcc cgcagagcct gggggaggtt
    11041 cacgatatgt tgtacgataa atcccttgag agggataact gtggtttcgg cctgatcgcc
    11101 cacatagaag gcgaacctag ccacaaggta gtgcgtactg caatacacgc actggcccgc
    11161 atgcagcacc gtggcgcgat tctcgccgat ggtaaaaccg gcgacggttg cggcttgctg
    11221 ttacaaaaac cggatcgctt ttttcgcatc gttgcgcagg agcgcggctg gcgtttagca
    11281 aaaaactacg ccgtcgggat gctattcctg aataaagatc ctgaactcgc cgctgccgca
    11341 cgccgcatcg ttgaagaaga actgcaacgc gaaaccttgt cgattgtggg ctggcgtgat
    11401 gtccccacta acgaaggcgt gctgggtgaa atcgccctct cctctctgcc acgcattgag
    11461 caaatttttg tgaacgcccc ggcaggctgg cgtccgcgcg atatggagcg ccgtctgttt
    11521 atcgcccgcc gccgcattga aaagcgtctc gaagccgaca aagacttcta cgtctgtagc
    11581 ctgtcgaatc tggtgaacat ctataaaggt ctgtgtatgc cggcggatct gccgcgcttt
    11641 tatctggatc ttgcggacct gcgtctggaa tcggccatct gcctgttcca ccagcgcttc
    11701 tccactaaca ccgtaccgcg ctggccgctg gcgcagccgt tccgctatct ggcgcataac
    11761 ggtgaaatca acaccatcac cggtaaccgc caatgggcgc gtgcgcgtac ctataaattc
    11821 cagacgccgc ttatccctga tctgcacgac gcagcgccat tcgtcaacga aaccggttct
    11881 gactccagct cgatggataa catgctggaa ctgctgctgg caggcgggat ggatatcatc
    11941 cgcgctatgc gtctgttagt gccgcccgcc tggcagaaca acccggatat ggacccggag
    12001 ctgcgtgcct tcttcgactt taactccatg catatggagc cgtgggatgg cccggcgggc
    12061 atcgtgatgt ccgacggtcg ttttgccgcc tgtaacctcg accgtaacgg tctgcgtccg
    12121 gcgcgctacg tcatcaccaa agataagctc atcacctgcg cctctgaagt cggtatctgg
    12181 gattatcagc ctgacgaagt ggtcgaaaaa ggccgcgtcg ggccaggcga actgatggtg
    12241 atcgacaccc gcagtgggcg tattctgcac tcggcagaaa ccgatgacga tctgaaaagc
    12301 cgccatccat ataaagagtg gatggagaaa aacgtccgcc gactggtacc gtttgaagat
    12361 ctgcccgatg aagaagtggg tagccgcgaa ctggacgacg acacgcttgc cagctaccag
    12421 aaacagttta actacagcgc ggaagagctg gactccgtaa ttcgcgtact gggcgaaaac
    12481 ggtcaggaag cggtcggttc gatgggcgat gataccccat tcgccgtgct ctccagtcag
    12541 ccgcgcatta tttacgacta cttccgccag cagtttgccc aggtgactaa cccgccaatc
    12601 gacccgctgc gtgaagcgca tgttatgtcg ctcgccacca gtatcggtcg tgaaatgaac
    12661 gtcttttgcg aagcagaggg ccaggcgcac cgtttaagct ttaaatcgcc gattctgctc
    12721 tactccgatt tcaaacagct cacgacgatg aaagaggagc actaccgcgc agatacgctg
    12781 gatatcacct ttgacgtcac taaaaccacg ctcgaagcga cagtcaaaga gctgtgcgac
    12841 aaagccgaaa aaatggtacg tagcggcacc gtgctgctgg tgctctccga ccggaatatc
    12901 gctaaagatc gcctgccggt tccagccccg atggcggttg gcgcgatcca gacccgtctg
    12961 gtcgatcaaa gcctgcgttg cgatgccaac atcatcgtcg aaaccgccag cgcccgcgat
    13021 ccgcaccact tcgccgtgtt gctgggcttc ggcgcgacgg ctatttatcc ataccttgcc
    13081 tatgaaacgc tgggccgcct ggtagacacc catgcgattg ccaaagatta tcgtaccgtg
    13141 atgctcaact accgtaacgg catcaacaaa ggcttgtaca aaatcatgtc caaaatgggc
    13201 atctccacca tcgcctctta ccgctgctcg aaactgtttg aagcggtcgg tctgcacgat
    13261 gatgtagtgg gcctgtgctt ccagggggcg gtcagccgca ttggtggggc gagctttgaa
    13321 gacttccagc aggatctgct gaacctgtcg aaacgtgcct ggctggcgcg taagcccatc
    13381 agccagggcg gcctgctgaa atacgtccac ggcggcgaat accacgctta caacccggac
    13441 gtggtgcgca cgctgcaaca ggcggtacaa agcggcgagt acagcgacta tcaggaatac
    13501 gcgaagctgg ttaatgagcg tccggcaacc acgctgcgcg atctgctggc aattacgccg
    13561 ggtgaaaacg cggtcaacat tgctgatgtt gaaccggcaa gcgaactgtt taaacgcttt
    13621 gataccgccg cgatgtctat cggcgcgtta agcccggaag cccacgaggc gctggcggaa
    13681 gcgatgaata gcatcggcgg taactcgaac tccggtgaag gcggcgaaga cccggcgcgc
    13741 tatggcacta acaaagtgtc gcgcatcaag caggtggctt ctggtcgctt cggcgttact
    13801 ccggcgtatc tggtcaatgc cgacgtcatt cagattaaag tcgcccaggg cgcgaagccg
    13861 ggcgaaggcg gtcagttgcc gggcgacaaa gtcactcctt acatcgccaa actgcgctat
    13921 tcggtgcccg gagtgacgct gatctccccg ccgccgcacc acgatatcta ctctatcgag
    13981 gacttagcgc agctcatttt cgacctcaag caggttaacc cgaaagcgat gatctccgtg
    14041 aagctggttt ccgaaccggg cgtaggcacc atcgcgactg gcgtggcaaa agcttatgcc
    14101 gatttaatca ccatcgcagg ctatgacggt ggcaccggcg caagcccgct gtcgtcagtg
    14161 aaatacgcag gttgcccgtg ggagctgggg cttgttgaaa cccagcaggc gctggttgcc
    14221 aatggtctgc gtcataagat ccgtttgcag gtcgatggcg gcctgaaaac cggtgttgat
    14281 atcatcaaag cggcgattct cggcgcagaa agcttcggct tcggcactgg cccgatggtg
    14341 gcgctcggct gtaaatatct acgtatttgc catctgaaca actgcgcaac gggtgtagca
    14401 actcaggatg acaaactgcg taagaaccac tatcacggcc tgccgttcaa ggttaccaat
    14461 tactttgaat ttattgcccg tgaaacccgc gagctgatgg cacagctggg cgtaacgcgt
    14521 ctggtggatc tgattggtcg caccgacctg ctgaaagaac tggacggttt caccgccaaa
    14581 cagcaaaaac tggcgctgtc gaagctgctg gagaccgccg aaccgcatcc aggtaaggca
    14641 ctctactgca ccgaaaacaa tccaccgttt gataacggcc tgctgaacgc gcagttgctg
    14701 caacaggcga aaccgtttgt cgatgaacgc cagagcaaaa ccttctggtt cgatattcgc
    14761 aacaccgacc gttctgtcgg cgcgtcgctt tcaggctata tcgcccaaac gcacggcgat
    14821 caggggctgg cagccgatcc tatcaaagcg tacttcaacg gcaccgcagg ccagagtttc
    14881 ggtgtgtgga acgcgggcgg cgtggaactg tacctgaccg gggatgccaa cgactatgtc
    14941 ggtaaaggca tggcgggcgg cttaatcgcc attcgtcctc cggttggttc cgccttccgc
    15001 agccatgaag caagtattat cggcaacacc tgcctgtatg gcgcgaccgg tggtcgtctg
    15061 tatgccgcag gccgcgcggg tgaacgtttc ggcgtgcgga actccggtgc tatcaccgtg
    15121 gtagaaggca ttggcgacaa cggctgtgaa tatatgacgg gcggtattgt ctgtattctg
    15181 ggtaaaaccg gcgttaactt cggtgcgggc atgaccggcg gcttcgctta cgttctcgat
    15241 gaaagcggtg atttccgcaa acgcgttaac ccggaactgg tcgaggtctt aagcgttgac
    15301 gatctggcga tccatgaaga gcatctgcgc ggtcttatca ccgagcatgt tcagcatact
    15361 ggctctcagc gcggtgaaga gattctggcg aactggtcaa ccttcgccac taaatttgcg
    15421 ctggttaaac cgaagtccag tgatgtaaaa gcactgctgg gtcatcgtag tcgtagcgca
    15481 gcagagttgc gcgtgcaggc gcagtaaggg gtagcaacaa tgagtcagaa tgtttatcaa
    15541 tttatcgacc tgcagcgcgt tgatccgcca aagaaaccgc tgaagatccg caaaattgag
    15601 tttgttgaaa tttacgagcc gttttccgaa ggccaggcca aagcgcaggc tgaccgctgc
    15661 ctgtcgtgcg gcaacccata ctgcgagtgg aaatgtccgg tacacaacta catcccgaac
    15721 tggctgaagc tcgccaacga ggggcgtatt tttgaagcgg cggaactgtc gcaccagacc
    15781 aacaccctgc cggaagtttg cggacgagtc tgcccgcaag accgtctgtg cgaaggttcc
    15841 tgcactctga acgatgagtt tggcgcggtg accatcggca acattgagcg ttatatcaat
    15901 gataaagcgt tcgaaatggg ctggcgtccg gatatgtccg gcgtgaagca aaccggtaaa
    15961 aaagtggcga ttatcggcgc aggcccggca ggtctggcgt gtgcggatgt cctgacgcgt
    16021 aacggcgtaa aagccgttgt cttcgaccgt catccagaaa ttggcgggct gctgaccttc
    16081 ggtattccgg ccttcaagct ggaaaaagag gtaatgacgc gtcgccgtga aatcttcacc
    16141 ggcatgggta ttgaattcaa actcaatacc gaagtgggcc gcgacgtgca gctggacgat
    16201 ctgctgagtg attacgatgc cgtcttcctt ggcgtcggga cttatcagtc aatgcgcggc
    16261 gggctggaaa acgaagacgc cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac
    16321 accaaacagt taatgggctt tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc
    16381 aaacgcgtgg tggtccttgg cggtggcgac actgcgatgg actgcgtgcg tacgtccgtg
    16441 cgccagggag cgaagcacgt tacctgtgcc tatcgtcgtg atgaagagaa catgccgggt
    16501 tcccgccgcg aagtgaaaaa cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag
    16561 ccactgggga ttgaagtgaa cggtaacggt aaagtcagcg gcgtaaaaat ggtacgtacc
    16621 gaaatgggcg aaccggatgc caaaggccgt cgccgcgcgg agatcgttgc aggttccgaa
    16681 catatcgttc cggcagatgc agtgatcatg gcgtttggtt tccgtccaca caacatggaa
    16741 tggctggcaa aacacagcgt cgagctggat tcacaaggcc gcatcatcgc cccggaaggc
    16801 agcgacaacg ccttccagac cagcaacccg aaaatctttg ctggcggcga tatcgtccgt
    16861 ggttccgatc tggtggtaac cgctattgcc gaaggtcgta aggcggcaga cggtattatg
    16921 aactggctgg aagtttaagc tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg
    16981 aaattctcct ttctttctgg aaagcgcctc gcataatctg aagaaaacca atgtaatacg
    17041 ctcatctgga ttgttaccgg tagatgcccg gcgtatagct gattccatta ttcaacgcag
    17101 cgccatataa agggacagat aatggaatcg ctctcagaga gaacctcaac aggctaccag
    17161 caaatccacg acggtattat tcatctggtc gatagcgccc ggacggaaac ggtacgtagc
    17221 gttaacgcgc tgatgaccgc gacatactgg gaaattggcc gacgaattgt cgaatttgaa
    17281 caaggtggcg aggccagggc tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat
    17341 ttaagtctaa ggtataagcg tgggttctct gcaaaaaact tacgccaaat gaggcttttt
    17401 tacctctttt ttcaacatgt tgaaattcgc cagacagtgt ctggcgaatt aacaccattg
    17461 ggaattccgc agacaccatc tgcagaattc ccatcggcaa agatttggca gacgctgtct
    17521 gccaaatctt tccccctgcc ctggtccacc tacgtccgtt tactttccgt taaaaacgct
    17581 gacgcccgca gcttttatga aaaagagacg ctccgctgtg gctggtctgt tcgccagcta
    17641 gaacggcaaa ttgcgaccca attttatgag cgaatactac tgtcacatga caaatcagcc
    17701 atgctgcaac aacacgctcc taccgagacg catattcttc cgcaacaggc gatacgcgat
    17761 ccctttgtgc tcgaatttct ggaattgaaa gatgaatatt cagaatctga tttcgaggag
    17821 gcgctgatca accacctgat ggatttcatg ctggaacttg gggatgattt tgcctttgtt
    17881 ggtcggcagc gaagattacg cattgatgac aactggtttc gtgtcgacct gctgtttttc
    17941 caccgccgtt tacgctgcct actaatcgtc gatctaaaag tgggcaaatt cagctatagc
    18001 gatgccggac agatgaatat gtatctcaac tacgccaaag agcactggac gctaccggat
    18061 gaaaatccgc ccatcggtct ggttctctgt gcagagaaag gagccggaga agcacattat
    18121 gctctggcag gtttgcctaa caccgttctg gcaagcgaat ataagatgca actacctgat
    18181 gagaaacgac tcgcagatga actcgttcga acacaggcgg tgctagagga aggctataga
    18241 cgccgttaat ttcagcggtt tcgaattctg cagacagtgt ctgcagaatc tttaaaacga
    18301 actaagtttt aagccattaa atcagcctta accttcacca ccaccttttt aatctcaccc
    18361 ggctcgccaa caacgcatcc cggcttatgc ggctctcctg gcataaacac ggcgaacatt
    18421 cccggtttta agatgatggt ttgctcgttt tcaatggcgc tgcaaagctg ataatcatcc
    18481 tcatggtgga actcttcaca ctgacgcgcg gtgcctgcca tgccaaacag aatccgttcc
    18541 tcaccgttta ataacagctg gatatcaatg tattgctcgt gcaattccgc ttttttctcg
    18601 acgggcgatt gagtgttaaa cgtcatgaca ttcataaaga tattgtcgcc ctgtaattcg
    18661 taacgacccg gcgctttttc ttgcggtctg gcagctaatg ccagcgttaa cgcgtcctgt
    18721 aacgcaggat gtaacccagc agacggtaat gactgtactt cacccatcat cataattttt
    18781 ctccctgggc caacagcgca gccccaagta aacctgcatc atggcggtag tgcgccgcca
    18841 gtaaatcaac atgaaatgcc gctggctcct gcgccagata cgtttccacc agcgccagat
    18901 acccttctgc cagcccaacg ctgccaccga ccaccacgca ctggcaatca gttgtggctt
    18961 taatatcagc gatcagcctt gccagcacat gtgcggagcg gtgaatcagt tgctgcgcct
    19021 gctcgtcgcc ctgcccggcg cgtgtgaaaa tagttctcgc atccgcgccc atgagttcac
    19081 cttgcgctgc cgccgcaatg ccgcgaccag aagcaatcgc ttccacgcaa cctgtgcgtc
    19141 cacagccgca gactgggccg tgtggatcgg caagcgtatg cccgatatgc cccgccagac
    19201 cgccagggcc ggtaagcagt ttaccgccgc tcactacacc gccgccgacg ccagtggaaa
    19261 cggtgataaa caccatctcg gttacatcgc cttccagcgc ctgatactct gcccatgctg
    19321 cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt aagttgttcc agcgttttga
    19381 ctaacggaaa gtgtagcaat ccaccgagat tatgcggatt cagcgccagc aaactgccgt
    19441 cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc atgagcttgc aacggagaga
    19501 ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt
    19561 cacgacgatc gcggatctgc ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc
    19621 cgatatcaat cgccagtgtg gtcatagcac cgcctttttc atcgctgtgt tgtaccactg
    19681 acaaatgtgt tcaagacgcg taatggcaga accgaccgtc accgcccacg cgccgtggcg
    19741 catcgcatca gccgcctgag caggcgtgtt gtaacgccct tcggcaatca cccgacatcc
    19801 ggcgtcgctc aacgttttca ccagcgccag atccggctct tctggcgttt caggcgtggt
    19861 atagccagaa agcgtagtgc caataatttc ggctcccagc ttttggcatg ccaggccgtc
    19921 ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga tggtgaatac gtgccagcag
    19981 cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc
    20041 cgcctgcgcc agcgcatcga catcttcaat ataggccgtg atgcgtaccg gagaatcctc
    20101 cagatcgcgt ttcacaatcc cgataattgg cacgctcacc accgcacgcg tggcttgcag
    20161 atttgccaca ccttcaatgc gaatggcaac cgcgcccgcc tgctctgccg ctaatgccat
    20221 ggcggcgacg atttcgggtt tatcgagcgg gctgtccgga accggctggc aggagacaat
    20281 caggccaccg ttagcagcga ttttttgatc cagttgtgca agtaacgaca tacatcttcc
    20341 cttagcgaaa ggcccggtac atagaccggg caacaggatt aacttttggt tttgactaaa
    20401 tcgtttttgg ggctgccaaa tggcacggca ccgctaaatg gtttgccgtc gatagcgtca
    20461 tgagtacgca atgcttccgg gcgcagccaa cgctgaacgc gggaaggcat atccagacca
    20521 atcagcagga tcaccacaaa tgtcagactg aacgagagcg atgccagcgc agtacccaga
    20581 tccagacgtt gagcgatcaa cgcgccgatg attggggcca gtgcgccgcc caatgcgcca
    20641 acgttgtagg taaagcccag gcccgctgca cgctggtcgg tatcgaaata accgccaatc
    20701 agtttcggta agatcccggc gatcccttgt ccaagcattt gctggaagaa cagtaacaga
    20761 ccgagcaccc agacgtttgc gccgccaatc gcaaataccg gaataatcag cagctgcgag
    20821 gccagcaggc tacaaacgta cgctttgcgg gttcccagcc agtcaccgag gaagccacct
    20881 acgcagcatc ccacggccgc gccaaagcca ctaaagaaca gcacattggc gacagtatgc
    20941 gggttataag ccagatcggt tttcagatac gttggcagca gcgcctgaat cggccatgag
    21001 tagaggaaag caaacaacac gaccaccatc agcattacgc ccgttggcca gcgtttaccc
    21061 gtactttgca ccataaagct gataaagatt gcggcgcata acagcccgag aacagcgacg
    21121 attgcagcat tttgcaggtt accggcgaag cagaaccaca gcgcagtagc cgccgccagt
    21181 gtcattacga tattggcaat gcgatgttca ccacggtaga gaatatccac cattgtgcgt
    21241 actggtgctt tacctgcgtg tttctctttc cagtcttccg cttccgggat gtttttacgc
    21301 agccagagag caaagatgat tggcaaaatg ccgataaaga acagcgcacg ccaaccccag
    21361 accggaacca ccaggctata gacctgagcg gcaacgacgg cccccacaga gaagcctgaa
    21421 atcaaaaaac cactggcttt gttacgcaga tgttttggcc agctttcaat gacataggtg
    21481 gcgctggaac cgtattcacc cgccatcccc atgccgatga ccagacgagc gataaacatg
    21541 gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg
    21601 gtgaccattg ccagacgacg cccgtagcgg tcacccatag cgccgagcat caggccgccg
    21661 aaccagcgag agataaaggc tgcggagatc agacttgccg cctgcaccgt cgtcagcccg
    21721 aattcaccct gtacttcggt gagtaccagg gcgattaaaa cgaaatcaaa accgtcaagc
    21781 agatatccca accaggcagc ggaaaatgcc cgccattgtg cacgattgag atggcgatac
    21841 cacgggatat tctgggttgt agtactcatt gtgagtctcc cgcggtgggc gatgcccaca
    21901 cgctttggta tgaaaattgt agggtacaga tgcgtttatt tcccctcacc cggtaggggc
    21961 gagcgagggg aaacaactca cccgcgctct tgcatcaact gctgggccag cgccttcagt
    22021 tctggcagat atttttcatc taccggacca aacggtttgc ggcacagcgg cacagaaacg
    22081 acatccatat aatgcaggac agttttcagg ccgcggaata cgcccgtttt gatcagtaaa
    22141 tcaatgactt tattacattc ggtttgcagc ttctgcgcgg tctggatatc gccttctttc
    22201 agcgccttca caatcccctg ataacgccag cccatgatgt tgtaggtact accgatacca
    22261 ccatcagcgc ccgccagaag accagaggcg aagatttcgt cgtaaccgtt atagagcacc
    22321 agatcaggat gttcacgacg gatctgctcc atctgataga gatcgccaga ggtctgtttc
    22381 agcgcaccta cgccaggcaa cgtaacaagt gtgttgatct gatccagggt cagttttacc
    22441 ccactcaggg ctggaatgtt gtacaccacc atcggcaaac catccgccga atcaataatt
    22501 gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg
    22561 gagacggcat cgaagccata acgtttagcc gatgccgcaa gttgttggct ttcggcggtg
    22621 ctgacgcaac cgacgtgggc gatgagttta atctgacctt tcgcctcttc ggcgacgatt
    22681 tccagtacct gttcacgctc ggaaaggctt tgtacaaagg cctcgccggt cgaaccaccc
    22741 acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa ccaggcgacg cagactcgct
    22801 ttatccagtg cttgttgttg gtcaaaagga gtcaggagtg cagccattac gccacgtaaa
    22861 ttcgttgcca taaatacctc tgaagtgatg cttgtctgat aaacgatata cctttatacc
    22921 tgttatacca gatcaattaa gcaacacccc atacagaaag cttataatgc gatctgcttc
    22981 actaaagtgg cattatttct ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga
    23041 gacgctgttg agatgcgatt gcaacgcacg atcggcttcg tcaggatcat gacggcggat
    23101 cgcatcaacg atcgcaatat gctgttgata actaacgttg ttatgttcgt gcagtgcctg
    23161 atcggtaacc gttgggcgtg cggcaataag ccagtcgagc agggcaacgt ggatcgccat
    23221 gaagattggg ttaccgggga tctccgccag cacgcggtgg aaatcaacgt ctgaacgaat
    23281 gaatgccgcg ttgttatcca gcgactgact gttgatttcc agtgcttttg ccagcaaatc
    23341 gatttgctca tcggtggcat gttcagccgc atagcgcacc aggctggatt caaagaacag
    23401 acgtaattgt tcgaaatggg caatcccacc aggatgagaa aggaaatctt tcgccatgcc
    23461 ggaaagctcg ccgatgatgg tgtccgcaga aggacgtgag acgcgggcac gttcgccgtt
    23521 gttgatttgc accagacctt tgcgttttaa cgctgccagc gcttcgcgca cagaaggacg
    23581 cccgacgtta aagaacgcca tcagttcgcg ttcagacggt aattgttcac cttcgccaaa
    23641 ttcacgacgg cggatcatct gttccagctc ttcttccacc atttcggaga gttttttacg
    23701 cgccagcggg cggctacgca agttgcgacc aattgcaggt gaagaatctt cggtttgcga
    23761 atcaaatgcg ttcataaggc ccattctgta aggtcagtgt gattaacatc atcagtgaca
    23821 tcctatcaca ggattgaaag taggggaaaa tggcaggttt ttctctttgt gcctcatcat
    23881 taccataatt aacggaataa ttaactattg cgaaaaatta atgtaacgca gataaaaaca
    23941 tcccgtttga attatttata agactattca cgagcattat gaatattatg aatgtgttct
    24001 tacaaaataa tcataagcgc atatttttta atgaaaaatc acctcaccta caattaaaaa
    24061 cacgacatcc gcaccataaa tagacttgca aaaaatataa catcattgtt ttcaatctgc
    24121 cgtttatggg attgaccgtt ttcttttgac acggagttca acaatgttcg gcataattat
    24181 atctgtcatc gtattaatta cgatgggcta tttgatcctg aaaaactaca aacctcaggt
    24241 gctgctggct gccgcaggta tcttcctgat gatgtgcggt gtctggttag ggttcggtgg
    24301 tgtactcgat cccgccaaaa gcagcggcta cttgatcgtc gatatttata atgaaatcct
    24361 gcgcatgctg tccaaccgca ttgccggatt ggggctgtcg attatggcgg tgggcggtta
    24421 tgcccgctac atggagcgca caggagccag tcgcgcgatg gtgagcttgc taagccgccc
    24481 gttaaaactc attcgctcgc cgtatattat tctgtcggca acttacgtca tcggccaaat
    24541 catggcgcag tttattacca gcgcctccgg cctgggtatg ttgctgatgg tcaccttatt
    24601 tccgacgctg gtgagtctgg gagtaagtcg cctctctgcg gtggcggtta tcgcaaccac
    24661 gatgtccatt gagtggggga ttctggaaac gaactccatt tttgcagccc aggtcgcggg
    24721 aatgaaaatt gccacttact tcttccacta ccagcttccg gtcgcctctt gcgtcattat
    24781 ctcggtggcg atctcccact ttttcgtgca acgcgctttt gacaaaaaag ataaaaatat
    24841 caatcacgaa caggcagagc taaaagctct cgataatgtc ccgccgctct attacgccat
    24901 tttacctgtg atgccgttaa tcttgatgct cggctcgctg ttcctcgccc acatcgggct
    24961 gatgcagtca gaactgcatc tggtggtggt gatgttactg agtttgacgg tgacgatgtt
    25021 tgttgagttc ttccgcaagc ataacttgcg cgaaacaatg gacgatgtgc aagcgttttt
    25081 tgacggcatg ggtacgcagt ttgccaacgt ggtaacgctg gtggtcgcgg gtgaaatatt
    25141 tgcgaaaggc ttaacgacga ttggcactgt tgatgcggtt atcaggggtg cggagcattc
    25201 tggtctgggc ggtattggcg tgatgattat tatggcgcta gtcattgcca tttgtgccat
    25261 tgtgatgggc tctggcaatg cgccgtttat gtcatttgcc agtcttattc cgaatatcgc
    25321 agccggatta catgtaccag cggttgtaat gattatgccg atgcattttg ccacgacgct
    25381 agcgcgcgcg gtttcgccga ttactgcggt ggtggtcgtt acgtcaggaa ttgcaggcgt
    25441 ttcgcctttt gcggtggtga agcgaacagc gatccccatg gcagtcggtt tcgtggtgaa
    25501 tatgattgcc acaatcacgc tattttatta agccattaaa agacaaaaca ggccaccagg
    25561 gcctgttttt gtattacttc acaacgcgta atgccggtcg accaccgcgt ggtggctgcg
    25621 gaagttcatc gtcaggatga gtgtcatcat cgtgatctgg cttgtcgcca tcaataaccg
    25681 acataacggt ttcgttgtct gccgatgcct cttcatcatt catgatgctg gtatcttcat
    25741 cgtaggcagc ttcaggctca aacatcgtgc ctgcgccatt ttcacgggcg tagatagcca
    25801 gcacggcagc cagcggcaca gaaacctgac gcggaatgcc gccaaagcgc gcgttaaagc
    25861 gtacctcatc attcgccagt tccagattgc cgacagcacg cggcgcaatg ttgagtacga
    25921 tttgcccgtc acgcgcatat tccataggaa cctgcacgcc agggagcgtc acatccacca
    25981 ccaagtgcgg cgtgagctgg ttatccagca accactcata gaatgcacgc agcagatagg
    26041 gacgacgtgg tgttagctgt gacaaatcca tacagattaa ctccggccca gacgcatttc
    26101 acgttctgct tcagttaaag aagcaaggaa agagtcacgc tcaaagacgc gggtcatata
    26161 gcctttcagc tctttcgcac ccgggccgct gaactcgatg cccagttgcg gcagacgcca
    26221 cagcagcgga gcaagatagc aatcgaccag gctgaactca tcgctcagga agtacggctt
    26281 ctgaccgaag accggcgcaa tcgccagcag ttcttcgcgc agttgcttac gtgcggcatc
    26341 tgcttcagaa gctgaaccgt tgatgatggt gttcatcagc gtgtaccagt ctttttcgat
    26401 gcgatgcatg tacagacggc tttcaccgcg agctaccggg taaacaggca tcagtggcgg
    26461 atgcgggaaa cgctcatcca gatattccat aatgatgcga gattcccaca gggtcagctc
    26521 acgatccacc agggtcggaa cgctctgatt cgggttgagg tcaatcagat cctgaggcgg
    26581 attgtccttt tccacgtgtt cgatctcgaa acttacacct ttctcagcca gcacaatgcg
    26641 gacctgatgg ctatagatgt cagtaggacc ggaaaacagc gtcattaccg aacgtttgtt
    26701 ggcagcgaca gccatgaaaa cctccaggta tagtcagaat ttttactgct accagccacc
    26761 aggtggccag tcagaagttg tgttacccag taaggaacga ctctctttgt tcgaaaatca
    26821 aacaaaaaat gagcaatacc cgacatttgg gcagaaaatt ggatgatagt ttaccagatt
    26881 ttgcgacctt tgtggtgagt cgatgccgga aatggggaaa aagagatgcg ctttagtctg
    26941 aaatagttga cttagtccct tattggcgat gtggtttttg ttttgcctgt ctgtcagacg
    27001 gtagcaaaaa gcaactttcc aatttttacg ctgattcaga atttaaccat aaaaaaaccc
    27061 gccgaagcgg gttttttcgc aaattgtttt ctgccggagc agaagccaat taacgtttgg
    27121 agaactgcgg acgacgacgt gctttacgca gaccgacttt cttacgttca acctgacgag
    27181 cgtcacgagt aacgaagcca gctttacgca gttcagaacg cagggactcg tcgtattcca
    27241 tcagagcgcg ggtgataccg tgacggatcg caccagcctg accagagata ccaccacctt
    27301 taacggtgat gtacaggtcc agtttctcaa ccatgtcgac cagttccagc ggctgacgaa
    27361 ctaccatgcg agcagtttca cgaccgaagt actgttccag agaacgttgg ttgattacga
    27421 ttttaccgtt gcccggtttg atgaaaacgc gagctgcgga acttttgcgg cgaccagtgc
    27481 cgtagtattg attttcagcc attgcctata atcccgatta gatgtcaaga acttgcggtt
    27541 gctgtgccgc gtggttgtgc tcgttacccg cgtaaacttt cagtttacgg aacatagcac
    27601 gacccagcgg gccttttggc aacatgcctt taaccgcgat ttcaatcaca cgctcaggac
    27661 ggcgagcaat catctcttca aaggtcgctt gtttgatacc accgatgtgg ccggtgtggt
    27721 gatagtacac tttgtcagta cgcttgttgc cggttacagc aactttgtca gcgttcagaa
    27781 cgatgatgta atcaccggta tctacgtgcg gagtgtattc cgctttgtgc ttaccgcgca
    27841 ggcgacgagc cagttcagta gccagacggc ccagagtttt accggtcgcg tcaacaacat
    27901 accagtcgcg ttttacggtt tctggtttag ctgtaaaagt tttcattaaa agcttaccca
    27961 ataaatagtt acacgttggt gaacacccaa acgtcttcaa ttgttgaggt tcacacgaca
    28021 aagtccggca aacctacccc ttcgaatagc ctatgccagc acacaaaaag ttttgggaaa
    28081 aaaactttct tgtaacgtgg ggtcgcagga ttatagagaa gtcggggtca aagatcgacc
    28141 cctttttgtg atttgtgaca ggttttaacc cgccaaatgc tcgcgcttca gatactcttc
    28201 gctttgcatc tcttgcagac gtgacaggca acgctggaac tcaaacttca gccgatcgcc
    28261 ctgataaatt tcatacagcg gcacttctgc actcaccact aatttgacat ggcgctcgta
    28321 aaactcatcc accagcgcaa taaagcgccg cgcttcgctc tccatcaacc gcgtcataac
    28381 tggtacatca aacaacatga ccgtatgaaa gagacgtgag agcgcaatat agtcatgctg
    28441 actgcgggcg tcgacgcaca gcgtagtaaa agagaccgcc agcgtctggt tctcgacgcc
    28501 cattgtcgcc aatggccgat ggttgatttc taacgtcggt gaattttctc gtttcgcccc
    28561 cgccagcgcc aaccatagtt tatccatctg cgcccgggtt tcatcgttga gtggcgaaag
    28621 ccacagatgc gcctgagtga gtgtacgcag tcgataatca acaccagcgt ccacgttcat
    28681 tacatcacaa tgctgtttaa tggcatcgat tgcaggcaga aaacgcgcac gttgcaggcc
    28741 atttcgataa agttcatccg gcggaatatt tgacgtcgct accagggtaa tgccgcgggc
    28801 gaacagggct ttcatcagac cgccaagtag catggcgtcg gtaatatcag aaacaaaaaa
    28861 ttcgtcaaaa cagagcacgt cagtttcggc tttaaagcga tcggcaataa tttccagcgg
    28921 atcggtctgc ccctgtaagg cagttagctc ttcgtgcacc cgcagcataa aacggtgaaa
    28981 gtgcaggcgc tgtttccgct ctcccggcag gctttgatag aaaaggtcca tcagccaggt
    29041 tttcccgcgt cctacaccgc cccacatata taagccacgc actggcgtat gctttgtgtc
    29101 ttcgcgttta ccccacagct taccgacccg cgccattagc ccactcgtcc tgggggctgg
    29161 tggcctgcta ttgatgagtt cttgataaat aatttccagg cggctgacgg cctctttttg
    29221 aacgtcgtcg ggttgatggc tgccttcatt gagcgccttc aggtattgcg atgttggggt
    29281 aacgctttgc atgatcttat tgttattcct tgaataatcg gtgcgccgtt gttcacggtt
    29341 gacgaaaaaa aggccgttct acactacgcg atatgcagtc gggattccac ttctgtggaa
    29401 ttaacggtta tagtggcata atcagccgca ggcatggagc ctgaagccaa caccctacgg
    29461 aaacaaaaga caacgggaga tgttcatgac ctgggaatat gcgctaattg ggttagtcgt
    29521 cggcatcatt attggtgctg tggccatgcg ttttggtaat cgtaaactac gccagcaaca
    29581 ggcgttgcag tacgaactgg aaaagaataa agctgaactg gacgagtatc gcgaagagct
    29641 ggttagccac tttgcccgca gcgcggaatt actggatacc atggcgcacg actatcgcca
    29701 gctgtatcag cacatggcga aaagctccag cagcctgctg ccggaactgt ctgctgaagc
    29761 aaacccgttc cgtaatcgtc tggcagagtc tgaagccagc aacgatcagg caccggtgca
    29821 gatgcctcgc gactattctg aaggcgcatc cggcctgctg cgtactggcg cgaagcgcga
    29881 ctaatttatt tttcgggcgc agccattgcg ccctcctctt ctctccctcc ccgactatca
    29941 tttaatctgg tgtctcattg ttagccgtct gaaaattcaa taacatcaaa ctgttttgaa
    30001 tctcttttct tatcattcag gtacgagagc aggaataatg aaaaaacaaa cccagctgtt
    30061 gagtgcatta gcgttaagtg tcgggttaac tctctcggcg tcatttcagg ccgtcgcgtc
    30121 gattccaggc caggttgccg atcaggcccc tctccccagt ctggctccaa tgctggaaaa
    30181 agtgcttccg gcagtggtga gcgtacgggt ggaaggaacg gccagtcagg gacagaaaat
    30241 cccggaagaa ttcaaaaagt tttttggtga tgatttaccg gatcaacctg cacaaccctt
    30301 cgaaggttta ggttccggtg tcatcatcaa cgccagtaaa ggctatgtgc tgaccaacaa
    30361 ccatgtgatt aatcaggcac agaaaatcag tattcagctc aatgatgggc gcgagtttga
    30421 tgcaaaactg attggtagcg atgaccagag cgatatcgcc ctgttacaaa ttcaaaaccc
    30481 gagcaaatta acgcaaatcg ctattgccga ctccgataaa ttgcgcgtcg gtgattttgc
    30541 cgtagcggtc ggtaacccat ttggccttgg gcaaaccgcc acctctggca ttgtttccgc
    30601 attaggccgc agcgggttga atcttgaagg tctggaaaac tttatccaga cagatgcttc
    30661 cattaaccgc ggtaactccg gcggtgcact attaaacctt aacggtgagt taattggcat
    30721 caacactgca atccttgcgc ctggcggcgg gagcgtcggg attggatttg ccatccccag
    30781 taatatggcg cgaacactgg cgcagcagct tatcgacttt ggtgaaatca aacgcggttt
    30841 gttaggcatc aaaggcaccg agatgagtgc cgatatcgcc aaagccttca accttgacgt
    30901 gcagcgtggc gcgtttgtca gcgaagtgtt gccaggttct ggctcggcaa aagcgggcgt
    30961 caaagcgggc gatattatta ccagcctcaa cggcaaaccg ctgaatagct ttgctgagtt
    31021 gcgctctcgt atcgcgacca ccgagccggg cacgaaagtg aagcttggcc tgctgcgtaa
    31081 cggcaaacca ctggaagtag aagtgacgct cgataccagc acctcttcgt cggccagcgc
    31141 tgaaatgatc acgccagcgc tggaaggtgc aacgttgagc gatggtcagc taaaagatgg
    31201 cggcaaaggt attaaaatcg atgaagttgt caaaggaagc ccagctgctc aggctggctt
    31261 gcaaaaagac gatgtgatca ttggcgtcaa ccgcgatcgg gtgaactcga ttgctgaaat
    31321 gcgtaaagtg ctggcggcaa aaccggccat catcgccctg caaattgtac gcggcaatga
    31381 aagcatctat ctgctgatgc gttaatgtcg taaaccgggc atcaggctta cgtgtgatgt
    31441 ccggttaact cgtggtatgc tgctgccgtt ccctttttta atgacgcctc catcatgttt
    31501 gtgaagctct tacgttccgt tgcgattgga ttaattgtcg gcgctattct gctggttgcc
    31561 atgccttcgc tgcgcagcct taacccgctt tccactccgc aatttgacag taccgatgag
    31621 acgcctgcca gttataatct ggcggttcgc cgcgccgcgc cagcggtggt taacgtttac
    31681 aaccgtggtt tgaacaccaa ctctcacaac cagcttgaga tccgcacctt gggatccggt
    31741 gtaatcatgg atcaacgcgg ttatatcatc accaataaac acgtcatcaa cgacgccgat
    31801 cagatcatcg tcgccttaca ggatggtcgt gtatttgaag cattgctggt gggatctgac
    31861 tctctaaccg atctggcggt acttaaaatt aatgccactg gcggtttacc taccattccg
    31921 atcaatgcac gtcgcgtacc gcacattggc gacgtggtac tggcgatcgg taacccgtac
    31981 aacctcgggc agaccattac ccaggggatt attagtgcca cgggtcgaat cggtctgaac
    32041 ccgaccgggc ggcaaaactt cctacaaacc gatgcttcca ttaaccacgg taactctggc
    32101 ggcgcgctgg tgaactcgct gggagaactg atgggcatta acacgctgtc gtttgataag
    32161 agtaacgatg gcgaaacgcc ggaaggtatc ggctttgcga ttcctttcca gttagcaacc
    32221 aaaattatgg ataagctgat ccgcgatggt cgcgtgatcc gcggctacat tggtatcggc
    32281 ggacgcgaga tcgcaccact gcacgcgcag agcggtggta tagatcaact gcaagggatc
    32341 gtggttaatg aagtgtcacc tgacggccca gcggcgaatg cgggtattca ggtcaacgat
    32401 ctgattattt cggtggataa caaaccggcc atctctgctc tggagacgat ggatcaggtg
    32461 gcggaaattc gccctggttc ggtgatcccg gttgtagtga tgcgtgatga taagcagtta
    32521 acgctgcagg tcaccattca ggaatatccg gcaaccaatt aagtcgagtg ctcaaaacaa
    32581 aaaccggagt ctgtgctccg gttttttatt attcgctaat caattactta ttaacgaact
    32641 cttcgcccag ggcgatatct ttcttcagcg tatccagcat accttccagc gcgttctgtt
    32701 caaatgcgct cagggtaccg atagatttac gctcttccac gccgttttta cccagcagca
    32761 gcggttgaga gaagaaacgg gcgtactgac cgtcgccttc aacgtaggca cattcgacaa
    32821 cgccttgttc gccctgcagt gcacgaacca gagacagacc aaaacgtgca gctgcctggc
    32881 ccatagacag ggttgcagac ccgccaccgg ctttcgcttc aaccacctca gtacccgcgt
    32941 tctggatacg tttggtcaga tcagccactt cctgctcggt aaaactaacg ccaggaacct
    33001 gtgacagcag cggcagaatg gtaacaccag agtgaccgcc aataaccggc acttcaactt
    33061 cgcctggctg tttgcctttc agttccgcaa caaaggtgtt ggaacgaatg atatccagcg
    33121 tggtaacgcc gaacagtttg tttttgtcat aaacaccggc ttttttcagc acttcagcag
    33181 caattgcaac agtggtgtta accgggttag tgataatacc aatgcacgct ttcgggcagg
    33241 ttttcgcaac ttgctgtacc aggtttttca cgatgccggc gttaacgtta aacaggtcgg
    33301 aacgatccat acccggttta cgcgctacac ctgcagagat aagaacgaca tctgcgcctt
    33361 ccagcgccgg agtcgcatct tcaccagaaa aacctttgat tttcacagca gtagggatat
    33421 ggctcagatc gacggccaca ccgggagtca ctggagcgat atcatacaga gagagttctg
    33481 aacctgaagg cagttgggtt tttaacagta gtgcaagcgc ctggccaata ccgccagcag
    33541 cgccgaggac tgcgactttc atcctaaact ccttattata ttgataaact aagatatgtt
    33601 gctccgctgc cgcgacctta atccacaaaa ttgccgttta caatgaccac atctcaagaa
    33661 tgtgtagtca cgcaagttta gcgtttatgc atttaattgc cgtaatcagg aacctaactt
    33721 acgtaattaa cagtcgctta tgcattagcg caacattcca acaggtggtg acaatatacc
    33781 ctaccgttca gccaaaacaa catcaatttg ataacaatta atttactttt aagcagaatt
    33841 tgcatgccgt gacgcaggca tgtttctcaa taacgaaatt tgataaaatc ccgctctttc
    33901 ataacattat ttcagccttc ttcagggctg actgtttgca taaaaattca tctgtatgca
    33961 caataatgtt gtatcaacca ccatatcggg tgacttatgc gaagctcggc taagcaagaa
    34021 gaactagtta aagcatttaa agcattactt aaagaagaga aatttagctc ccagggcgaa
    34081 atcgtcgccg cgttgcagga gcaaggcttt gacaatatta atcagtctaa agtctcgcgg
    34141 atgttgacca agtttggtgc tgtacgtaca cgcaatgcca aaatggaaat ggtttactgc
    34201 ctgccagctg aactgggtgt accaactacc tccagtccat tgaagaatct ggtgctggat
    34261 atcgactaca acgatgcagt tgtcgtgatt cataccagcc ctggcgcggc gcagttaatt
    34321 gctcgcctgc tggactcact gggcaaagca gaaggtattc tgggcaccat cgctggcgat
    34381 gacaccatct ttaccacccc tgctaacggt ttcaccgtca aagacctgta cgaagcaatt
    34441 ttagagctgt tcgaccagga gctttaatct ctgccccgtc gcttctggcg gcggggaaaa
    34501 tgttgcttat ccctctcatt ccccttcttt ctcctgcgat acatttaacg aatagtgcgt
    34561 tttaccgcga cacatcattc acaaaatgaa tacatacggt aaaaaaagca cattatgcaa
    34621 aattcattat ctaattgaaa atactataat taataataaa taaccgtgtt ttttatactt
    34681 ttttgttatt aaaattcaca tttttaacac ttagtatcaa ctgaaacagt tagcgtggta
    34741 ttaattagtt caataattag tgtatacttg attttgtgat atgggtcacg aaacaaagac
    34801 ccagctaaaa gattatgtcg aggtaaaaat catgaaaatc aaaaccactg tagctgcatt
    34861 aagcgtactt tctgttctct ctttcggtgc attcgctgcc gactccattg atgctgcaca
    34921 agcacaaaat cgtgaagcaa tcgggaccgt atccgtaagt ggtgtggcgt cttcgccaat
    34981 ggatatgcgt gaaatgctga acaaaaaagc ggaagagaaa ggcgcaacgg cctaccagat
    35041 tactgaagct cgtagcggtg acacctggca cgctacggct gaactgtaca aataaaccct
    35101 catcgtcttg tccgacgata ttgcccccgg ttcgggggct ttttttcgcg ctaatgacga
    35161 acattaaaac gcaaatgccc ttccagctct tcctctgcct catcaaacag caatattaac
    35221 gcgccaaaac ggcgacgcgt tttctctccc agatgaacaa attcaatctc aagtggtagc
    35281 ggcaggacat cgttcattaa cacatcccat agtgagtcga gatcgcgtac cttatctttc
    35341 gccagaccaa aggtttggct aaagtcacga taaaaatcct cctgactctc aatctcatca
    35401 aaatcaaagg tataaatatt catctgttgc caccgtcacg tttcgcggcg agttctgccg
    35461 tgcatcgcta agtatagcca tgaaaaaacc gacgcttttg gcgtcggttt tgacttaact
    35521 atcggtcaac gcatgttgat acttatggag catccccgcc agccgatgaa c
//
LOCUS       DAFATA010000039        35569 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFATA010000039 DAFATA010000000
VERSION     DAFATA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.039"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(262..588)
                     /locus_tag="LGJ20_002315"
     CDS             complement(262..588)
                     /locus_tag="LGJ20_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBK2386369.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            723..1064
                     /locus_tag="LGJ20_002316"
     CDS             723..1064
                     /locus_tag="LGJ20_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBK2386370.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1099..1659
                     /gene="speG"
                     /locus_tag="LGJ20_002317"
     CDS             1099..1659
                     /gene="speG"
                     /locus_tag="LGJ20_002317"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HBK2386371.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(1662..2372)
                     /locus_tag="LGJ20_002318"
     CDS             complement(1662..2372)
                     /locus_tag="LGJ20_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBK2386372.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2480..2785
                     /locus_tag="LGJ20_002319"
     CDS             2480..2785
                     /locus_tag="LGJ20_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBK2386373.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            2984..5410
                     /locus_tag="LGJ20_002320"
     CDS             2984..5410
                     /locus_tag="LGJ20_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBK2386374.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            5471..7894
                     /locus_tag="LGJ20_002321"
     CDS             5471..7894
                     /locus_tag="LGJ20_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBK2386375.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            7905..8522
                     /gene="dmsB"
                     /locus_tag="LGJ20_002322"
     CDS             7905..8522
                     /gene="dmsB"
                     /locus_tag="LGJ20_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBK2386376.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            8524..9378
                     /locus_tag="LGJ20_002323"
     CDS             8524..9378
                     /locus_tag="LGJ20_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBK2386377.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            9421..10035
                     /gene="dmsD"
                     /locus_tag="LGJ20_002324"
     CDS             9421..10035
                     /gene="dmsD"
                     /locus_tag="LGJ20_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBK2386378.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            10194..11486
                     /gene="clcB"
                     /locus_tag="LGJ20_002325"
     CDS             10194..11486
                     /gene="clcB"
                     /locus_tag="LGJ20_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBK2386379.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(11439..12134)
                     /gene="bioD"
                     /locus_tag="LGJ20_002326"
     CDS             complement(11439..12134)
                     /gene="bioD"
                     /locus_tag="LGJ20_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBK2386380.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(12259..13479)
                     /gene="mlc"
                     /locus_tag="LGJ20_002327"
                     /gene_synonym="dgsA"
     CDS             complement(12259..13479)
                     /gene="mlc"
                     /locus_tag="LGJ20_002327"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBK2386381.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(13614..14507)
                     /locus_tag="LGJ20_002328"
     CDS             complement(13614..14507)
                     /locus_tag="LGJ20_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2386382.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            14614..15867
                     /locus_tag="LGJ20_002329"
     CDS             14614..15867
                     /locus_tag="LGJ20_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2386383.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            16264..16599
                     /locus_tag="LGJ20_002330"
     CDS             16264..16599
                     /locus_tag="LGJ20_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBK2386384.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            16692..16775
                     /locus_tag="LGJ20_002331"
     CDS             16692..16775
                     /locus_tag="LGJ20_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386385.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            16875..17696
                     /locus_tag="LGJ20_002332"
     CDS             16875..17696
                     /locus_tag="LGJ20_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBK2386386.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(17735..18064)
                     /gene="mdtI"
                     /locus_tag="LGJ20_002333"
     CDS             complement(17735..18064)
                     /gene="mdtI"
                     /locus_tag="LGJ20_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBK2386387.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(18051..18416)
                     /gene="mdtJ"
                     /locus_tag="LGJ20_002334"
     CDS             complement(18051..18416)
                     /gene="mdtJ"
                     /locus_tag="LGJ20_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBK2386388.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            18828..19862
                     /gene="tqsA"
                     /locus_tag="LGJ20_002335"
     CDS             18828..19862
                     /gene="tqsA"
                     /locus_tag="LGJ20_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBK2386389.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     DVLLSDLNKE"
     gene            complement(19887..21275)
                     /gene="pntB"
                     /locus_tag="LGJ20_002336"
     CDS             complement(19887..21275)
                     /gene="pntB"
                     /locus_tag="LGJ20_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBK2386390.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(21286..22818)
                     /gene="pntA"
                     /locus_tag="LGJ20_002337"
     CDS             complement(21286..22818)
                     /gene="pntA"
                     /locus_tag="LGJ20_002337"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBK2386391.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            23342..24286
                     /locus_tag="LGJ20_002338"
     CDS             23342..24286
                     /locus_tag="LGJ20_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2386392.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            24472..25854
                     /locus_tag="LGJ20_002339"
     CDS             24472..25854
                     /locus_tag="LGJ20_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2386393.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            25891..26613
                     /gene="folM"
                     /locus_tag="LGJ20_002340"
     CDS             25891..26613
                     /gene="folM"
                     /locus_tag="LGJ20_002340"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBK2386394.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(26610..26945)
                     /locus_tag="LGJ20_002341"
     CDS             complement(26610..26945)
                     /locus_tag="LGJ20_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBK2386395.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            27074..27793
                     /gene="rstA"
                     /locus_tag="LGJ20_002342"
     CDS             27074..27793
                     /gene="rstA"
                     /locus_tag="LGJ20_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBK2386396.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALNFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            27797..29098
                     /gene="rstB"
                     /locus_tag="LGJ20_002343"
     CDS             27797..29098
                     /gene="rstB"
                     /locus_tag="LGJ20_002343"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBK2386397.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            29174..30103
                     /gene="tus"
                     /locus_tag="LGJ20_002344"
     CDS             29174..30103
                     /gene="tus"
                     /locus_tag="LGJ20_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBK2386398.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(30100..31503)
                     /gene="fumC"
                     /locus_tag="LGJ20_002345"
     CDS             complement(30100..31503)
                     /gene="fumC"
                     /locus_tag="LGJ20_002345"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBK2386399.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(31646..33292)
                     /gene="fumB"
                     /locus_tag="LGJ20_002346"
     CDS             complement(31646..33292)
                     /gene="fumB"
                     /locus_tag="LGJ20_002346"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBK2386400.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            33491..34666
                     /gene="manA"
                     /locus_tag="LGJ20_002347"
     CDS             33491..34666
                     /gene="manA"
                     /locus_tag="LGJ20_002347"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBK2386401.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            34767..>35569
                     /locus_tag="LGJ20_002348"
     CDS             34767..>35569
                     /locus_tag="LGJ20_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HBK2386402.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVN"
BASE COUNT         8792 a         9131 c         8716 g         8930 t
ORIGIN      
        1 gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc
       61 gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcta ctaccataca
      121 agtatgaaga tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga
      181 atggttgaca aaagatgaaa tagaatacct tttgtcagct gacacttcct cttatcttat
      241 tgataaaatg gatttatgtt cctacgtgcg cccccagccc gcaacaatga tcaacatgcc
      301 gcaaagcgca atcaacgcac ccgtccagtc ataaagagtc agtttcacgc catccacaac
      361 gcgcagccac atcaacgccg tgcagacata aacgccacca taagccgcgt aaacacgccc
      421 actcgccgct ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa tccccgccgg
      481 aagcaacagc cagatactgg cgtttcgttt taaccacaac cagggcagaa agcatccaat
      541 aatttcacac agcgcagtag caaaaaatag taacgttgtt ttaatcatct ttgtcactta
      601 ttgacatcat gtatagttat agggcgacat aatatcatca atataaacac cctcctggta
      661 cgttttgcgt tcgcagtgga tggtgtagaa tctcctttaa tcattcatac agggaatgaa
      721 ttatgaaaat cactctcagc aaacgaatcg gcctgctcgc ttttctgctg ccttgcgcac
      781 tggcactgag cacaactgtt catgccgaaa ctaacaaact ggtgattgag tctggcgaca
      841 gtgcacaaag ccgccagcac gccgctatgg aaaaagagca atggaatgac acgcgcaatc
      901 tgcgtcagaa agtgaataaa cgcactgaaa aagagtggga taaagccgac gccgcttttg
      961 ataaccgcga taaatgtgag caaagcgcca acatcaatgc ctactgggag ccaaatactt
     1021 tgcgctgcct ggaccgtcga actggccgcg ttattacccc ctaacctgtt attgatttaa
     1081 ggaatgtaag gacacgttat gccaagcgcc cacagtgtta agctacgccc gctggagcgt
     1141 gaagatttac gctatgtaca tcaactcgac aataacgcca gtgtgatgcg ttactggttt
     1201 gaggaaccct acgaagcctt tgttgaactc tctgatctgt atgataagca tattcacgat
     1261 cagagcgaac gacgctttgt ggtggaatgt gacggcgaaa aagccggtct ggtggagctg
     1321 gtggaaatta accatgtaca tcgccgggca gaatttcaga taattatctc cccggagtat
     1381 caggggaaag gtctggcaac ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt
     1441 ctcaatctct ataagctgta tctgatcgtc gataaagaga atgaaaaagc gattcacatt
     1501 taccgcaagc ttggctttac ggttgaaggt gaattgatgc acgagttctt tattaatggt
     1561 caatatcgta atgccattcg catgtgtata ttccagcatc agtatctggc agagcacaaa
     1621 acaccgggtc agactctcct gaagccgacc gcacaatagc attaataata atcgatcgta
     1681 tttttgatgg tgtaaacccg ttcgacggcg ggttttactc cttcatcaac aatgatcagc
     1741 tgacagtcca ccggattggc gtgactgtca tattcacagc tctgttttac attaccaact
     1801 cgttgattat tcagtaaagt aaccgctgtg taatctaatt ttttgatcgg atccgttgat
     1861 ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg tggttttacc cagcggataa
     1921 ccctgatcat catagcgata ttccatctgc atttgtttgc tgctggcttt aatcacgaag
     1981 ccattatcat cggtttccca actcaccccg gcagaaggta attctgctag ctggcatttt
     2041 ccctgtaaac gtactctctt ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt
     2101 accagagcga ccacggtatt attttccaga tccagtaatt cgagtgaatc aaaacagcct
     2161 tcttccgaca aagtcccaga aacacgtttc gtcacttcac cttgctcatc cattaatgtc
     2221 tgagtgaaat cttttaccgg accacgcagc ggatcaaaat cgaattcatt agagaaactg
     2281 gccatctcag gggtaaatga aagtgttacc tctgtgcggt cacatcctgt gaggaatatc
     2341 gcgagtaagc atggtagtaa tttgtatttc acaacagtca ccagagagta gagatgattc
     2401 tcaatcatag tagcaaatac agtactttac acgttaaatg ctatgcttaa agaagttatc
     2461 ttcgcgtaag gagcttatga tgaaactctc aacctgctgt gccgcgcttc tgctcgccct
     2521 cgcctcaccc gcggtactcg ccgcgccggg atcctgtgag cgcatacaga gcgatatatc
     2581 acagcgcatt atcaataatg gcgtacccga aagcagcttc acgttaagta ttgtacccaa
     2641 tgaccaggtt gatcagcctg attcccaggt cgtcgggcat tgtgctaatg atacgcataa
     2701 aattctctac acccgcacca ccagcggtaa cgtctctgct cccgcgcagt ctacccagga
     2761 tggcgcgcct gccgaaccgc agtaatacat tatcgactga acgccggata cgacacatcc
     2821 ggcgatttga acatacaaca taatcccacc ttattactca tacccttcta ttgatatgga
     2881 ttaataattc ttaacccaaa atgggtagac tccctctatt gttagcgcgc taaatattca
     2941 atatataaac atttatataa cgataaagaa cagggagtga gttatgtcca aaaatgaccg
     3001 aatggtgggc atcagccgca gaacactcgt taaatcaacc gcgataggtt ctctggcgct
     3061 ggctgcaggc ggtttttctt taccgttcac cctgcgcagt gcagcggcag cggtacaaca
     3121 ggccagcgaa aaagtgatct ggggtgcctg ttccgtcaac tgtggtagcc gctgtgcact
     3181 tcgtctacat gttaaagata atgaagtgac ctgggtggaa actgacaata ccggcagcga
     3241 tgagtacggc aaccatcagg tacgcgcctg tttgcgcggt cgctccatcc gccggcgtat
     3301 taatcatccc gatcgcttga attacccaat gaaacgcgtg ggtacacgcg gtgaaggcaa
     3361 attcgaacgg attagctggg atgaagccct ggatactatc gccagtagcc tgaaaaaaac
     3421 cgtcgaacaa tatggcaatg aggctgtata tattcagtac tcttcgggga ttgttggcgg
     3481 caatatgacc cgctcttcgc catcagcctc ggcggtcaaa cgcctgatga actgctacgg
     3541 cggttcactc aatcagtatg gctcctacag caccgcgcaa atttcctgtg ccatgcccta
     3601 cacctacggc agtaatgatg gcaacagcac cacggatatt gaaaacagca agctggtcgt
     3661 gatgtttggt aacaacccgg cagaaacccg catgagcggc ggtggcatca cttatcttct
     3721 tgaaaaagcg cgcgagaaat cgaacgccaa aatgattgtt atcgatccgc gatataccga
     3781 tacggctgct ggtcgtgaag atgaatggct ccctattcgc ccgggcaccg atgccgcgct
     3841 ggttgcgggt attgcctggg tattgattaa cgaaaatctc gttgatcaac cttttctcga
     3901 taaatactgc gtcggctatg acgaaaaaac cttacccgca gatgcaccca aaaatggtca
     3961 ctataaagcc tatattcttg gtgaaggtga tgataacaca gcgaaaacgc cgcagtgggc
     4021 ttcgcaaatt accggtatcc cggtagaccg tatcatcaaa ctggcgcgtg aaattggcac
     4081 agcaaaaccc gcttatatct gccagggctg ggggccgcaa cgccaggcaa acggcgaact
     4141 gactgcacgc gctattgcta tgttacctat tttgacgggc aatgtcggca tcagcggcgg
     4201 aaatagtggc gcgcgtgaat cgacctatac cattaccata gaacgcctgc cggtgctgga
     4261 taatccggtc aaaacgtcaa tctcctgctt cagctggaca gatgctatcg atcatggtcc
     4321 gcaaatgacg gcaatccgcg acggcgtccg cggcaaagat aaactggatg tgcccattaa
     4381 gttcatctgg aactacgcgg gaaataccct cgttaatcag cattctgaca tcaacaaaac
     4441 gcatgaaatt ctgcaagacg aatcgaaatg cgaaatgatt gtggtcatcg aaaactttat
     4501 gacctcatcg gcaaaatatg ccgacattct gctgccagac ctgatgaccg ttgagcagga
     4561 agatattatt cctaacgact acgccggtaa catgggatat ctcattttcc tccagccagt
     4621 caccagcgaa aaattcgaac gtaaaccgat ttactggatc ctgagtgaag tcgcgaaacg
     4681 tcttggacct gacgtctatc aaaagttcac tgaaggtcgc acgcaggaac aatggttaca
     4741 acatctgtac gccaaaatgc ttgccaaaga tccggcgtta ccgtcttacg acgaactgaa
     4801 aaaaatgggt atctataagc gtaaagatcc caatggtcat tttgtcgcct acaaagcatt
     4861 tcgtgacgac cccgaggcaa atccacttaa aacaccttcc ggtaagattg aaatttattc
     4921 cagcaagctg gcggaaattg cccgtacctg ggaactggaa aaagatgaag tgataagccc
     4981 actgccagtt tatgcctcta cctttgaagg ctgggactcc cctgaacgta gaaccttccc
     5041 acttcaactg tttggtttcc attacaaatc ccgtactcac tcgacctacg gcaatattga
     5101 tctcctgaag gctgcctgcc gtcaggaggt gtggatcaac cctatagatg cgcagaaacg
     5161 tgggattgcc aacggcgata tggtgcgggt gtttaaccat cgtggcgaag ttcggctacc
     5221 agccaaagtg acaccacgca ttctccctgg ggttagcgct atgggccagg gagcctggca
     5281 cgaggccaat atgtctggcg ataaaatcga ccatggcggc tgtgtgaata cgctaaccac
     5341 tctgcgccct tcaccactgg cgaagggaaa cccgcagcac actaatctgg tcgagatcga
     5401 aaaaatataa cccacgacaa ccataaattc tggcatgaca ttttgtttga aaagcaataa
     5461 gtgagtaatg atgaaaatcc ataccacaga ggcgctcatg aaggctgaaa tcagccgtag
     5521 aagtctgatg aaaacctccg cacttggcag tcttgcgctg gcaagcagtg ctttcactct
     5581 gccattttct caaatggtcc gggcggcaga ggctccggta gaagagaaag cggtctggag
     5641 ttcctgcacc gttaactgcg ggagccgctg tctgttacgt ttgcatgtga aagatgacac
     5701 cgtgtactgg gtggagtctg atacgacagg tgacgacgtc tacggtaatc atcaggttcg
     5761 cgcgtgttta cgcgggcgct ctatccgccg acggatgaat catcctgata ggttgaaata
     5821 tcccatgaag cgcgtcggca agcgcggtga aggtaaattt gaacggataa gttgggacga
     5881 agccctggat accatcagtg ataatcttcg gcggatccta aaagattacg gcaatgaggc
     5941 tgtacatgtc ctgtacggaa caggcgtaga tggcggaaac atcaccaact caaacgtccc
     6001 gtaccgtctg atgaactctt gcggtggttt tctcagtcgc tatggcagct acagtaccgc
     6061 acagatcagt gccgcaatga gttatatgtt cggtgccaat gatggcaaca gcccggatga
     6121 tatcgccaat acgaaactgg tcgttatgtt cggaaataac ccggcagaaa cgcggatgag
     6181 cggcggtggt gtcacttact acgtcgagca agcccgcgaa cgttcaaacg cacgcatgat
     6241 cgtcatcgat ccacgttata acgacacagc tgccgggcgt gaagatgaat ggctgcccat
     6301 tcgcccaggc accgatggcg cactggcctg tgcgattgcc tgggtactga ttactgaaaa
     6361 catggtcgat cagccatttc tcgacaaata ttgtgttggt tacgatgaaa aaacgctgcc
     6421 cgccaacgca ccacgtaacg cgcattataa agcctatatt ctgggcgaag ggcctgacgg
     6481 catagctaaa acgccggaat gggcagcaaa aatcaccagc atcccggcag aaaaaattat
     6541 ccagttggca cgagagatcg gttcagcaaa acctgcttat atttgtcagg gttgggggcc
     6601 acaacgacat tccaacggcg agcaaacgtc ccgcgctatt gccatgcttt ccgttctcac
     6661 cggtaacgtc ggcataaacg gcggcaactc aggtgtacgc gaaggtagct gggatctggg
     6721 ggtagaatgg ttcccgatgc ttgagaatcc tgttaaaacg cagatttccg tctttacatg
     6781 gacagatgcc atcgaccatg gtacggaaat gaccgcgacc cgtgatggtg ttcgtggaaa
     6841 agaaaaactg gatgtcccca tcaagttttt atggtgctac gccagtaaca cattgatcaa
     6901 tcaacatggc gacatcaatc acacccatga ggtgcttcag gatgacagca agtgcgagat
     6961 gattgttggc attgaccact tcatgacggc ctcggctaag tattgcgata tcctgttgcc
     7021 cgacctgatg ccaacagagc aagaagacct tatctctcat gaatctgcag ggaatatggg
     7081 ctatgtgatc ctcgcccaac ccgcaacctc agcaaaattt gaacgcaaac ccatctactg
     7141 gatgttgagt gaagtcgcca aacgcttagg accagacgtt tatcaaacct ttactgaagg
     7201 tcgcagtcag catgaatgga tcaaatatct ccatgcgaaa acgaaggaac gtaaccctga
     7261 gatgcccgac tacgaggaga tgaaaacgac cgggatcttt aagaaaaaat gcccggaaga
     7321 acactacgtc gctttccgcg cattccgtga agatccacag gcaaacccgt tgaaaacacc
     7381 ttcggggaaa atcgaaattt attcggaacg actggcgaag attgcagata cctgggaatt
     7441 gaaaaaagat gaaattattc atccccttcc tgcgtatacc ccaggttttg atggctggga
     7501 cgatcccctg cggaaaacct atccactgca gttaacgggc ttccactaca aagcgcgtac
     7561 ccactccagc tacggcaata ttgatgtgtt acagcaggcc tgcccacaag aggtgtggat
     7621 caaccccatt gatgctcagg cacgcggtat ccgtcatggc gataccgtgc gggtatttaa
     7681 caataatgga gaaatgctga ttgccgcaaa agtgactccg cgtattctgc ctggcgttac
     7741 cgccatcggg caaggtgcgt ggcttaaagc ggatatgttt ggtgaccggg tcgatcacgg
     7801 cggcagtatc aatattctga cctctcaccg cccttcaccg ctggcaaagg gaaacccgtc
     7861 gcacagcaat cttgtccaga tcgaaaaggt ttaaggagta gcccatgacc acacaatatg
     7921 gattttttat cgattccagc cgctgtactg gctgtaaaac ctgcgaactg gcgtgcaaag
     7981 attttaaaga tcttggcccg gaagtcagtt tccgccgtat ttatgaatac gctggcggcg
     8041 actggcagga agataatggc gtctggcacc agaacgtgtt cgcttattat ctctccatct
     8101 cctgtaacca ttgcgatgac cccgcatgta caaaagtctg ccccagcggc gcaatgcata
     8161 agcgtgaaga tggctttgtg gtggttgatg aagatgtttg tatcggctgc cgctactgcc
     8221 acatggcttg tccgtacggc gcgccacagt acaatgctga aaaagggcac atgacgaagt
     8281 gcgatggttg ttattcgcgc gtcgccgagg ggaaacaacc catatgtgtc gaatcctgcc
     8341 cgctgcgtgc acttgagttc ggtccaattg aagagctgcg ccagaagcac ggcactctgg
     8401 ctgccgtcgc gccactgccg cgcgcgcatt tcacaaaacc caatatcgtt atcaaaccta
     8461 acgccaacag ccgcccgacc ggcgatacca ctggttatct ggctaatccg gaggaagtgt
     8521 aaaatgggaa atggatggca tgagtggcca ctggttatct ttaccgtatt gggtcaatgc
     8581 gtagtaggcg cgctgattgt tagcggaatt ggctggttcg ccgccaaaaa tgacgcagac
     8641 cgtcagcgta ttgtccgtgg catgtttttc ctctggctgt tgatgggcat cgggtttatt
     8701 gcctctgtca tgcacctggg atcgccactg cgagcgttta actcgctgaa tcgaatcgga
     8761 gcttctggtc tgagtaacga gattgctgcc ggttccatat tttttgccgt tggcggacta
     8821 tggtggctgg tggcggtcat cggtaaaatg ccgcaagcgt tggggaaact ctggttactt
     8881 gtcagcatgg cgctcggcgt cattttcgtc tggatgatga cctgcgtgta tcaaatcgac
     8941 accgtgccaa cctggcataa cggttacact acgctggctt tcttcctgac ggtattgttg
     9001 agcggtccaa tacttgctgc cgcaattctg cgggcagctc gcgttacatt taataccaca
     9061 ccatttgcca tcattagcgt tttggcgctg atcgcttgtg ccggggtgat tgtgctgcaa
     9121 ggtttgtctc tggcgtctat tcactcatcc gtgcaacaag ccagcgcact ggtaccagat
     9181 tacgcctcct tacaggtctg gcgtgtggta ttgttatgcg caggtcttgg atgctggctt
     9241 tgcccgctca ttcgtcgccg tgaacctcac gttgccggac ttattctggg gctaatcctc
     9301 atacttggcg gtgagatgat cgggcgtgtg ctcttttatg gcctgcacat gacagtagga
     9361 atggctattg caggttaaca aattggtgcg gggcaacccg cacatttcag gatgttagga
     9421 atgacccatt tttcacagca agataatttt tctgtcgcgg cgcgcgtgtt gggcgcgctg
     9481 ttttattacg ctccagagag tgcggaagcc gcacctctgg ttgcggtact caccagtgac
     9541 ggctgggaaa ctcagtggcc tctaccagaa gcgtcattag cgccgctggt gacggctttt
     9601 cagacacagt gtgaagagac tcacgcccag gcctggcagc gtttgtttgt cggcccgtgg
     9661 gcactgccgt ctccgccgtg ggggtcggtc tggctggatc gcgaatctgt gctgtttggc
     9721 gattcaacat tggcacttcg tcagtggatg cgcgagaaag gcattcagtt tgaaatgaag
     9781 caaaacgaac cggaagatca ttttggttca ctgctgttga tggctgcgtg gttggcggaa
     9841 aatggtcgcc agacagaatg cgaagaattg ctggcatggc acctttttcc gtggtcaaca
     9901 cgttttcttg atgtttttat cgaaaaagca gaacaccctt tctaccgtgc actgggtgaa
     9961 ctggctcgct taacgctggc gcaatggcaa tcacaactgt taattcctgt cgcggttaaa
    10021 ccgctgtttc gataggatca ccgtaatatt gccggatggt gacgattacg cgcaatccgg
    10081 caataatagg ttacagtgtc acgttttttt atctcttaaa gcacgcactg cttttgcggc
    10141 tggcctcttt tgccgcaaaa tagtcgcccg tgtttcattg cccatttctg ctcatgcatc
    10201 atctacacat ctatccggat ctgcgcacta tgttccgccg tctgcttatc gcaacagtcg
    10261 tcggtattct cgcggccttt gccgttgccg ggtttcgtca tgcgatgctg ctactggagt
    10321 ggttgttcct caataatgac tccggcagtc tggtcaatgc agcgacaaac ctttccccct
    10381 ggcgacggtt gctaactccg gcgctcggcg gactggcggc gggtttgttg ctgatgggct
    10441 ggcagaaatt tacccaacaa cgccctcatg cgccgaccga ttacatggaa gcgttgcaaa
    10501 ccgatggaca gttcgattac gcagcaagcc tggttaaatc gcttgcctcg ctgctggtag
    10561 taaccagcgg cagtgcaatt ggtcgcgaag gtgcgatgat tcttttagct gcccttgccg
    10621 cctcctgttt tgcccaacgt tttacgccac gccaggagtg gaaattatgg atcgcctgtg
    10681 gggccgcggc gggaatggct gcggcctatc gtgccccgct tgctggcagt ttatttatcg
    10741 ccgaagtgct gtttggcact atgatgttgg cctctctcgg cccggtgatt atttccgccg
    10801 tcgtggcatt gctggttagc aatctgatta atcatagcga cgcgttactc tacaacgtac
    10861 aactctcagt gacggttcag gctcgtgact atgcgctgat tatcagtaca ggtgtgctgg
    10921 caggtctgtg cggaccactg ttgttaacgt taatgaacgc ctgccatcgt ggatttgtga
    10981 gtctcaaact tgcgccgccc tggcaactgg cactaggcgg gttgatcgtg ggtctgcttt
    11041 ccctgttcac acctgcagtg tggggcaacg gctatagcac cgtacaatcc tttttaaccg
    11101 ccccaccact gttaatgatc attgccggga tcttcctttg taaactgtgt gccgtgctgg
    11161 cgagtagcgg ttccggcgca cccggtgggg tctttacacc gacgctattt atcggtcttg
    11221 ccattggcat gttgtatggt cgtagcctgg gattatggtt ccctgatggc gaagaaatta
    11281 cgcttttact cggattgacc gggatggcga cactgttggc ggcaaccacg cacgcgccga
    11341 ttatgtcgac gttgatgata tgtgaaatga ccggggagta tcagctactc cccggtttat
    11401 tgattgcctg cgtcattgcg tcggtaattt cgcggacgtt acaccgtgac tctatctacc
    11461 gccagcacac tgcgcagcat agctaagcgg atgtattgcc ccagttctcg ctgttcggcg
    11521 cgcggcagat aaggcagttc accaatgagc ggtgccggaa gttttttacc cagcacatca
    11581 atgatttccg cataatgcgc cagtcctggg ttgattcggt tagccaccca gccaatgagc
    11641 ggcagcccgt cgttggcaat cgcctgagct gttagcagtg catggttaat gcaaccttcc
    11701 tgaataccga caaccatcaa caccggcagt tgttcctgca ccacccattc agagagtgga
    11761 cgcaaatcat tcatcagact gcgccagccg ccagtccctt ctaccacgac atgatcgact
    11821 ttatcggtca ggtttgccag gccgtttgaa atgagggtgt aattgattgg gcaactgtgc
    11881 gccacgctac tttcttcttc gcttaacgcg ataggattaa ctgcttcata aggcagttcg
    11941 atggttgaaa cactctgcaa caccagggca tctttattac gcagcccttc gggtgtctct
    12001 ttgctcccct tcgctacggg tttatatccc gcaaccgttt ttccctggga ggctaacgct
    12061 tgtagcaatg cgcgggaaac caccgttttc cctacagaag tgtctgtacc ggtaataaag
    12121 aaacgcttca gcatcactaa ctccaccgtt atgcttcaca aatataaacc aggaaaataa
    12181 ttaaccttga aagtctaagt tatgctttcc tggcccaaat tgagatagcg caaattttgg
    12241 tagaacagtt aaaaaatgtt aaccctgcaa cagacgaatc aacaaagaac cgttatacat
    12301 cgcgtctttt accagtgcag cgcctgccat cgtgccctgg ttagaaaact gagtactctc
    12361 aacgctgatg tgctgactat acgcaggaag ggcctgctga cggatgctgt ctgagatgac
    12421 cgggaagagg atatctgccg ctttacttaa cggtgagcca atcagtattt tttgtgggtt
    12481 aaataaattc accatgatgg caagaatgcg ccctacatgc gcgcccaccc cggtaatgat
    12541 gtcttttgcc agcagatcgc cgcgcaatgc cgcctgacac aatgagtcca cggttaacgg
    12601 ttgtccatgt aacatcgagc tcatggattg attaagacgc agctgtgcca gctcaagaat
    12661 actgtccaca ctggcgatgg tttcgaggca gccgtgattc ccgcaataac agcgtttccc
    12721 atacgggtcg acctgtgtgt ggcctatttc cacgagacta ctgctgcctg cgtgtagcag
    12781 atgaccatcg gtaatgacgc ccgcccccac gttgtgatcg ataaccacct gaatcacatc
    12841 gcgcgccccg cgtgaggcac caaacaaggc ctctgccatc gtccatgcgc tgatatcatg
    12901 ctgaatataa accggaacgc cggtatgctg ctccagagcc tcgccgagcg gcatctcttt
    12961 tacatcctcg tagaacggca tgcgatgtac aatgccattt tccgtatcaa taattcccgg
    13021 cagggttatg gcaatcgaag ttagacgctc aagttttttc tggtggcgga taaaaaactg
    13081 atcgatatgg gaaatgatac gatccagcaa tggcgagtca tcttttaacg ccagttcctg
    13141 cgactcttcc accaccagtt tgctgctcag atcgcgcaga gcaaggaaaa tctccccgcg
    13201 actaatgcgc agagaaagat agtgccaggc ttcagtttca accaccagcc ccaccgccgg
    13261 acggccacgg ttccccgctt ctttgatttc cagctcttgc accaggtgtg cttcgagcat
    13321 ctcacggaca attttagtga tactggcagg agccagctgc gccagacggg aaagatcgat
    13381 acgcgagact ggaccaagct gatcaatcag gcgataaacc gcgcccgcgt tggtctgctt
    13441 tatttgatca atgtgcccag gctggttttc agcaaccacc gcatactccc tatattttcg
    13501 cgctccgaaa taatctgtag gctatggtga agcacttcaa tacgtgtcgt caaattttta
    13561 cttaggcatg tgattaacag cacatttttc gggctttttc gctgaaattt ccctcacctg
    13621 agagcattca gcagatgaat acgaaagttt cgcgcagctg gactttgttc atgatgtttc
    13681 ggccagacca accacatttc agaaaccgca tcctcttctg caatcggcac ccagcgcatt
    13741 tcgttgagct gaacacgttt aaatgacgca ggcaaaattg aaacacccag accggcggaa
    13801 accagaccga tgatggtcat tgcctcgccc acctcctgag tgatgacggg cgtcaaatgg
    13861 taacgtcgca tcagcccgag aatatcgtca tacagccctg tcccgacgtg cggatcaaaa
    13921 aagacgaagg gttctttcgc cagttcagcc agcgttacat tcgggttatt tgccagggga
    13981 tgatcgtgcg ggatcatcgc cataagcggt tcatggacga tgactgcgtg ttcaagcgtc
    14041 tccggtaacg ctgtgttacg cagcaatccc atatccagcg ttccttcaat gagcggagcg
    14101 atttgctcac gagtgttcat ttcgcgggtt tgtaaatgga catcgggata atcacggcga
    14161 aacagcgata acgtatcgga caccgcccga ataaaaggag cggacgaagt aaaaccaatg
    14221 cgcaactccc ccgcttcccc ctgatgcagt ctttcagcgc gagcggcagc gtcatctacc
    14281 atagacagga tttgccgact atctgcaaga aactgttttc ctgctgccgt cagcaataca
    14341 ctgcgattag ttcgtgccag cagtcgggca ccaatttgtt gctccagtgc ctgaatctgc
    14401 tgacttagcg gtggttgcga aatattcagg cgggcagcgg cgcgcccgaa atgcagctct
    14461 tccgcaacag caacaaagta acgcagatga cgaagttcaa tattcatatt taaaacatct
    14521 tatttgagat tattaatata ttagacagaa caattcgatt ttcctaacct atgtataagc
    14581 ctgatccaca ggcatattta gcaaggattt caagtgagcc gtactacaac tgttgatggc
    14641 gctccggcaa gcgacactga caagcaaagc atttctcagc caaatcaatt tattaaacgc
    14701 ggtacgccgc aatttatgcg cgtcaccctg gcgctgttct ctgccggact ggcaacattt
    14761 gcacttctct attgtgtgca gcctatcctt ccggtgcttt cgcaggagtt tggcttaacg
    14821 cccgcgaaca gtagtatttc actgtccatt tccacggcga tgttggctat tggtttgctg
    14881 tttactggcc ccctatccga tgccattggt cgcaaaccag tgatggtcac ggcgctactg
    14941 ttggcctcca tttgtacgtt actttcgaca atgatgacca gctggcacgg cattttgatt
    15001 atgcgcgcct tgattgggct ttcgttaagt ggcgtggcag ctgttggcat gacttatctt
    15061 agcgaggaaa tccaccccag tttcgtggcc ttttcgatgg ggttgtatat cagcggcaac
    15121 tcaattggcg gcatgagcgg gcgcttaatt agtggtgtct tcacggactt tttcaactgg
    15181 cgaattgctc tggcggcaat cggttgtttc gcgctagcct cggcgttgat gttctggaaa
    15241 atcctccctg aatcacgcca ttttcgcccg acttcgctgc gacctaagac gttgtttatc
    15301 aactttcgtc tgcactggcg tgaccgggga ttaccgttat tgttcgcaga aggctttttg
    15361 ctgatggggt cgttcgtcac gctgtttaat tacatcggct atcggttgat gctttccccc
    15421 tggcatgtca gtcaggccgt ggttggctta ttatcgctgg cttatttgac cggtacatgg
    15481 agctcaccca aagccggaac catgaccacc cgctacgggc gcggtccggt gatgttgttt
    15541 tcgacggggg ttatgctgtt tggtttactg atgaccttat tcagctcgct gtggctgatc
    15601 tttgccggaa tgttactctt ctcagcagga ttcttcgcag cccactcagt tgccagcagc
    15661 tggattggcc cccgcgcaaa acgcgctaaa ggccaggcct cctcgctgta tctgttcagt
    15721 tactatctgg ggtcgagtat tgccgggacg ctgggtggtg ttttctggca taactatggc
    15781 tggaacggcg tcggcgcatt tattgctctg atgctggtca ttgctctgct ggtcgggacg
    15841 cgtttgcatc gtcgtctgca cgcctgaaaa ataagcccgg actgcggtaa ctacacgtcc
    15901 ggacttattg cttgctcaaa ccaacgttaa tagccatcct aaaatagacg aagcgccagc
    15961 caattcccgc agcgcgtcta gcgtcatcag gattataagt acccaaataa acggattcat
    16021 tttgctgtgt gtcatttatt actgatgcgc agttattcta ccgctttgta agtagtaaaa
    16081 tagttaaccc gatcaagact actattattg gtagctaaat ttcccttaag tcacaatacg
    16141 ttattatcaa cgctgtaatt tattcagcgt ttgtacatat cgttacacgc tgaaaccaac
    16201 cactcacgga agtctgccat tcccagggat atagttattt caacggcccc gcagtggggt
    16261 taaatgaaaa aacaaattga gggtatgaca atgaaaaaag tattagctct ggttgttgcc
    16321 gctgctatgg gtctgtcttc tgccgccttt gctgcagaga ctgcgaccac acctgctccg
    16381 actgcgacga ccaccaaagc agcgccggcg aaaacgacac atcataaaaa acagcataaa
    16441 gcagcacctg cccagaaagc gcaggcggct aaaaagcatc ataaaaatac gaaagctgaa
    16501 cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac acgccgggaa acacagccat
    16561 cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag ttttcaacag taatgctggc
    16621 gcgccccctc gcgcctgaaa attacggtgc taagcgggta acgtttagca ccgcctttag
    16681 cggagggtag tatggtgggc cgttatcgct ttgagttcat tcttatcatc cttattttat
    16741 gcgcactgat tgccgcccgt ttttatcttt cctgactgta gttatctgat tttactccca
    16801 ctttcatccc gtcccgtcta tagtatttat gagggtttgc ttttaataat cataattacc
    16861 caccagagtg tgatatgcgt acaaccattg ctgtagtgtt gggtgcaatt agtttgacgt
    16921 ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc ggcaaacgat gaggtcagca
    16981 ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga cacgacccaa tcaccgtggg
    17041 atgcggttgg gcaactggaa acggccagcg gcaatttatg tacggcgacg ctgattgcac
    17101 ccaatctggc attaacggca ggacactgtt tattgacacc tccaaagggt aaagccgata
    17161 aagcagtggc gctgcgtttt gtgtcaaata aaggtctttg gcgctatgag atccacgaca
    17221 tagaaggccg cgttgatccg acactgggaa agcggttaaa agcagatggg gatggttgga
    17281 ttgttcctcc cgcagctgca ccatgggact tcggcttgat tgtgctgcgt aatccccctt
    17341 ctggcattac accgttgccg ttatttgagg gagataaagc cgcgcttact gccgcattaa
    17401 aatcggctgg tcgtaaagtg actcaggcgg gatatcctga agatcatctc gatacgttat
    17461 acagtcatca aaactgtgaa gtgactggct gggcgcaaac gtcggtgatg tcacatcagt
    17521 gcgatacctt gccgggtgac agcggttcgc ctctgatgtt gcataccgat gacggctggc
    17581 aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga tcgctggcgc gccgataacc
    17641 gggccatttc tgttaccggt tttcgcgaca agctggatca actgtcgcaa aaataatgtt
    17701 caaacgctgc ccgacagcgc gggcagcgtc ttcatcaggc aagtttcacc atgatcattc
    17761 cagccaacag caagaccagg ccaatccagc ctttacgatt taaccgttga ccaaacaaga
    17821 tccaacctgc ggctaacgtg gcggcaatac caaacccgcc ccacaatgca taagcgacag
    17881 acaagtcgat cccttttacg gcctgagaaa gcgcactaaa ggcagccagc accgccgcca
    17941 gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga aaatttcaaa aagacgttag
    18001 caacgatttc cagcacgatt gccaatgcca gccaggcggc gtgaacccat tcaaactgcg
    18061 ccatggttca cctccagttc aggtttacgc gctttacggg tacctgattt tatcaacaca
    18121 atcccggcga ccagggtggt taacccggca attttcatca gcgataaact ttcgtcgaat
    18181 aacaaaacgc tgaacaaggt aataaataaa ataccgatac cttcccacaa cgcataagct
    18241 acgcctaagg cgattttttt aacggcgaaa gagagaaata tatacgacag agaaatcatc
    18301 accagcatta aaataaagcc gccatttccc tcactgacgc tcgcccattt cattgacagc
    18361 gtaccggtaa tttctgtagc aatagccaga cctaataaaa tccaataaat atacattgtc
    18421 cttctcctgc aagagaatta ttttaatttt cgcttaattc agcgaaacca aagtaaagtg
    18481 gtcaagcccc gcgcataggg caagcttagg cagaagaaag gactaaagcg cgttgcgcca
    18541 gtgctgctca cctacgagca agatagtgga tgaggtacga agatgggtaa atgtagaaaa
    18601 taacgtcctg aacaaattgt ccataatatt acaattatcc gcagtgttgc ttctcgtcat
    18661 cgcggatgat aattgtcctc ggtagttgaa cacgcctgat ttgtatcata gcttaagaat
    18721 taactcaaaa tattttcact tctttacctg agcggtttga ttttcgttat gatgacggag
    18781 cgaaaaagac attattatta gcaaaggaag aaaaaccggg gacaagcatg gcaaagccga
    18841 tcatcacgct caatggccta aaaatcgtca ttatgttggg aatgctggtc attattctct
    18901 gcggtatccg ttttgccgcc gagatcatcg tgccgtttat tctcgcatta tttattgctg
    18961 ttattcttaa cccgctggtg caacacatgg tccgctggcg agtgccgcgt gtactggcgg
    19021 tgtcgatttt gatgaccatc atcgtgatgg cgatggtgtt gctattagct tatctgggtt
    19081 ccgcgctcaa cgagttgacg cggacgttac cgcaatatcg caactctatt atgacgccgc
    19141 tgcaagctct tgagccgttg ttgcaacgcg tagggattga cgtctcagtt gaccagctgg
    19201 cgcattacat tgatccgaac gcggcgatga cgttgctcac caacttattg acgcagttat
    19261 ctaatgccat gtcatcaata tttttattgc tgctgacggt gctgtttatg ctgctcgaag
    19321 tgccacaatt gcccggaaaa tttcagcaaa tgatggcgcg tccggttgaa gggatggcgg
    19381 cgattcaacg tgcaattgac agtgtttctc attatctggt gctgaaaaca gccatcagca
    19441 tcatcaccgg cctggtcgcc tgggcgatgc tcgccgcact cgatgttcgc ttcgcttttg
    19501 tctggggatt gctggccttt gcgcttaatt acatccctaa tattggttca gttctcgcgg
    19561 caatcccccc tatcgctcag gtactggtgt ttaatggctt ctacgaagcg ttgctggtgc
    19621 tggcgggata tctgctgatt aatctggtct tcggcaatat tctggagccg cgcatcatgg
    19681 gacgtgggct ggggctttcc acattggtgg tatttttgtc gttgattttt tggggatggt
    19741 tgttaggacc ggtgggtatg ctgctttccg tgccgttaac aattattgtc aaaattgcgc
    19801 ttgaacaaac agcgggaggt caaagcatcg acgttctgtt aagcgatctc aataaagagt
    19861 gacggcctcc gcagaggccg tcagggttac agagctttca ggattgcatc cacgctggct
    19921 ttggcgtcac caaacagcat gtgggtgttt tccttgaaga acagcgggtt ttgcacacca
    19981 gcatagccag tgttcatcga acgtttaaag acaatcacgt tctgcgcttt ccacacttcc
    20041 agaacaggca taccagcaat aggactcttc ggatcatcct gcgccgccgg gttaaccgta
    20101 tcgttagcac caatcaccag tacggtatcg gtatcagcaa agtcatcgtt gatctcgtcc
    20161 atttccagca cgatgtcata cggtactttt gcttcagcca gcaatacgtt catatgtcca
    20221 ggcaaacgcc ccgcaaccgg gtggataccg aaacgcacgt tgataccacg agcgcgcagt
    20281 ttctcggtaa tttcagcgac aggatattgc gcctgcgcga ctgccatgcc gtaccccgga
    20341 gtaatgatca ctgaatggga gtttttcagc agttccgctg tctcttctgc ggtgatttcg
    20401 cggtgctcgc ccacttcctg atcatcgcca gtagaagagc cgtcggtgcc gaaaccaccc
    20461 gcaataacgc tgataaagga acggttcatc gccttacaca taatgtaaga aaggatagcc
    20521 cccgaagaac cgaccagcgc accggtcaca atcagcaggt cgttgctgag cataaagccc
    20581 gcagccgcag ccgcccagcc ggagtacgag ttcagcatcg acaccaccac tggcatatct
    20641 gcaccaccga tggaggcgac taaatgccag ccgaatacca gcgcaatggc ggtcattatt
    20701 agcaatgcca gcacttgcag gccgacgctg tccgtgcgaa caaatacaat cagcagcagg
    20761 aaggaaacga ccagagccgc caggttcatt ttgtgacggt ttggcagcat caacggttta
    20821 gacgaaatct tgccacacag tttgccgaac gccaccaccg aacccgtgaa cgttaccgcc
    20881 ccgatgaaga taccgaggaa cacttccgtc aggtgaatat tgaccagaat cggtgccatt
    20941 cccgcgtcat gatgcagata gctgttaaag ccaaccagca ctgccgccag acccacgaag
    21001 ctgtgcagga tcgccaccag ttctggcatt tcggtcattt caactttctt cgccagacgg
    21061 ataccaattg ccccaccaat gaccatcgcc agcaagatcc agccaacatt acccgtatcc
    21121 ggtccaaaaa tggttgcgat taacgcaatc gccatcccgg cgataccgaa gttgttaccc
    21181 tggcgagacg tttcatgttt cgaaagaccg gccagactga agataaacag gatcgcggca
    21241 acaatgtatg cagctgtaac taatcctcca gacatatgtt accccttaat ttttgcggaa
    21301 cattttcagc atgcgctgag tcacggtgaa gccaccgaaa atattaatac tggctataag
    21361 caccgcgata aaactaagga agctaaccca gccgccctgg ccaatctgca acagtgctcc
    21421 gacaacaata atccctgaaa tcgcgttggt gaccgacatc aacggtgtat gcagcgcatg
    21481 cgatacattc cacaccacgt aataaccgac aacgcaggcc agcgcgaaaa cggtgaagtg
    21541 cccaagaaac tctttcggcg caacgcttgc cagccaaccg aaaaggatga ttgccagcgc
    21601 catcaatgcg tatttacgcc acggtgagca ggcacatttt tcctcagttt tcacttccgg
    21661 tgccgctttt tgtgccgcct gcggctgagc tgatacctga atcggcggtg ccggccaggt
    21721 aatttcgccc gcacggatca cggtcacgcc gcgaatcacc acatcatcaa aatcaacagt
    21781 gatattgccg tctttctctt tgcacaacag tttcagcaga ttaacgaggt tagtaccgta
    21841 aagctgtgag gattgcgtcg gcagacggcc cggaagatcg gtataaccaa tcactttgac
    21901 accattttcc gtagtgaaga tttcacccgg cacggtgtat tcacagttgc caccgttttg
    21961 ggctgccagg tcgacaatca cactgcccgc cttcatggag tcaaccattt cacgggtaat
    22021 tagcttcggc gctggtttgc ctggaataag cgcggtggtg acaatgatat cgacctcttt
    22081 tgcctgggcg gcaaagagtt ccatttccgc tttgatgaac gcgtccgaca tcactttggc
    22141 atagccatcg ccgctgcccg cttcctcttt aaaatccagc tcgaggaatt ccgcgcccat
    22201 actttgaact tgttctttca cttccgggcg ggtgtcgaat gcacgcacaa tcgcgccgag
    22261 actgtttgct gcgccaatgg cggccagacc tgcaacaccc gcaccaatca ccatcacttt
    22321 tgccggtggc actttcccgg ccgcagtaat ttgcccggta aagaagcgcc caaattcatg
    22381 tgccgcttca acaatggcgc gataaccggc gatgttcgcc atcgagctta gtgcgtccag
    22441 cgattgtgcg cgtgagatac gcggcacaga atccatcgcc atcacggtca cgttacgttc
    22501 cgcaagtttt tgcattaatt ccggattctg cgcaggccag ataaaactca ccagcgttgt
    22561 cccaggattc agtaacgcaa tttcatcatc taacggcgca ttgaccttca gaatgatctc
    22621 tgactgccag acgctattcc cttctacaat ttcagcgccc gcttgcacaa acgctttatc
    22681 gtcaaaactt gccagttgac ccgcgccgct ctctaccgcg acggtaaaac ccagtttcag
    22741 cagctgttcc actgtttttg gcgttgctgc aacacgggtt tcattggtta accgttctct
    22801 tggtatgcca attcgcatga tattcccttc catcggtttt attgatgatg gtttgcctgt
    22861 gtcaggagcc acacaagccg ctcatgtacg agctaaatgt tactccgtta aaataaatta
    22921 gtaacaaacg ccctataacg tactgaaaat tatgcctgtg atctagcgcc aaaaatcagt
    22981 atttcggcgt gaactcgcaa aatattaacg attcagccgt gatagtggga taaacacctt
    23041 agaacgccgg ataaagactg ataattgtct tcgacggtcg ggtaaaacga gacatcgccc
    23101 cggcacgaat cactacttaa cattaaatta acttatacaa ttcagttgct tcagtagtaa
    23161 tgatgctgat acggctgttt tttaagcata gacggtcatt tgagcaggat taaaattggc
    23221 ttaaggaatg tgatatgaaa aatgacgcag acagttacac cgtttaaatg caataatcag
    23281 ccacgtttct cgttaataac aataccagta cctggtttgc gcaaggcgaa ggattatttt
    23341 tatgaagctt aagaacaccc tcctggcgtc ggcactgctt tctgctacgg cattctccgt
    23401 taacgcagca acagaactga caccggagca agcggcagcg gttaaacctt ttgaccgtgt
    23461 agtggttacc ggtcgtttta atgctattgg cgaagcggtg aaagccgttt ctcgtcgcgc
    23521 agataaagaa ggtgccgcct ctttttatgt tgtcgacact tctgattttg gtaacagcgg
    23581 taactggcgt gtggtcgctg acctctataa agccgatgct gaaaaagcag aagaaacaag
    23641 taatcgcgta attaacggtg ttgtcgaact gccgaaagat caggctgttc tgattgaacc
    23701 gtttgacacg gtcaccgtcc agggcttcta tcgtagccag ccagaagtca atgatgccat
    23761 caccaaagca gcaaaagcga aaggtgccta ctctttctac attgttcgtc aaatcgatgc
    23821 caaccagggc ggcaaccagc gtattactgc attcatctat aaaaaagatg ctaagaaacg
    23881 tatcgtccag agcccggatg tgatcccggc agattccgaa gcaggacgtg cagctctggc
    23941 tgccggtggc gaagccgcga agaaagttga gatcccaggt gttgcgacta ccgcatcacc
    24001 aagttctgaa gtcggtcgtt tctttgaaac ccagtcatca aaaggcggac gttacactgt
    24061 cacgcttcct gacggcacta aagtcgaaga actgaacaaa gcgacagcag cgatgatggt
    24121 cccgttcgac agcatcaaat tctctggcaa ctacggcaac atgaccgaag tctcctatca
    24181 ggttgcgaaa cgtgccgcga agaaaggtgc taagtattac cacatcaccc gccagtggca
    24241 ggaacgtggt aataacctga ccgtcagcgc agatctgtat aaataactat tcagttactc
    24301 ctataaggcg gcttgatgcc gcctttcgca tttttagtca caaatttccc cgcgaccatt
    24361 gcatgcagac acatctcttc gtaaaatccc gcgcttcagt ggcaaactcc atttttatgc
    24421 gttttgccct aatatttatt cttcttatca cgttttaatc actggatatc gatggaaaag
    24481 aaactgggac tgagcgcact caccgcgctg gtattaagct caatgctggg cgcgggtgtt
    24541 ttcagtctgc cgcaaaatat ggcggcagtt gccagcccgg cagcactgct catcggctgg
    24601 ggtattactg gcgctggcat tttattgctg gcctttgcca tgctgatcct cacgcgcatt
    24661 cgtcccgaac ttgacggcgg tatctttacc tatgcccgcg aagggtttgg cgagctaatc
    24721 ggcttttgtt ccgcatgggg atactggctg tgcgcagtca tcgccaacgt ctcctatctg
    24781 gtgatcgttt tttccgcgtt aagctttttt acggacacgc cggaattgcg cctgttcggc
    24841 gatggtaata cctggcaatc gatagtcggt gcatcggctt tattgtggat tgttcacttt
    24901 ttgatcctgc gcggggtgca aaccgctgcc agcattaacc tggtggcgac actggcaaaa
    24961 ttgttgccgc tgggtctgtt tgttgtgctg gcgatgatga tgttcaaact ggataccttc
    25021 aagctcgact tcaccggact tgcacttggc gtacccgtgt gggaacaggt gaaaaacacc
    25081 atgctgatca ccctgtgggt tttcattggt gtggaaggtg ccgtggttgt ttctgcgcgt
    25141 gcgcgtaata aacgtgatgt tggcaaagcg acactgctgg cggttctctc cgctctgggc
    25201 gtttacttgc tggtaacgct gctttcgctg ggcgtggtgg cacgtcctga actggctgaa
    25261 attcgtaacc cgtcaatggc cggtctgatg gtggaaatga tgggaccatg gggcgaaatc
    25321 atcatcgctg ccggtttgat cgtttccgtt tgcggcgcgt acttaagctg gaccatcatg
    25381 gcggcagaag ttccgttcct ggctgccact cataaagcat tcccacgcat ttttgcgcgc
    25441 cagaacgcac aagctgcgcc atccgcctcg ctgtggttga ccaacatctg cgtgcaaatt
    25501 tgtctggtac tcatctggct gaccggttcc gattacaaca cgttgctgac catcgcctca
    25561 gaaatgattc tggtgcccta tttcctggtc ggcgcgttct tgctgaaaat cgcaacccgt
    25621 ccactgcata aagcggtagg tgtcggtgcc tgcatttatg gcttatggtt attgtatgcc
    25681 tctggtccaa tgcacttgct gttatccgtt gttctgtatg cgccggggct gctggttttc
    25741 ctgtatgcgc gcaaaacgca tacacatgat aatgtactga accgtcagga aatggtgctg
    25801 attggcatgc tgttaattgc ctcagttccg gcgacctgga tgctggtggg ataacgttca
    25861 tcccatcgtt ggatagcaaa ggagataacg atgggtaaag cccagccctt gccaatatta
    25921 attactggcg gaggtcgtcg catcggcctc gccctcgcat ggcatttcat taatcaaaag
    25981 caaccggtga ttgtcagcta tcggacacac tatccagcca ttgatggact gattaatgca
    26041 ggtgcgcagt gtattcaggc tgatttttcg accaacgacg gtgtgatggc gtttgccgat
    26101 gaagtactaa aaagcaccca tggtctgcgt gctattttgc ataacgccag tgcgtggatg
    26161 gcggaaaaac cgggtgcgcc actggccgac gtactggctt gcatgatgca gatccacgtt
    26221 aataccccat acctgctcaa ccatgcgctg gaaagattac tgcgtgggca cggacacgcc
    26281 gccagcgata tcattcactt taccgattat gtggtggagc gcggtagcga caaacatatt
    26341 gcgtatgctg caagcaaagc ggcactggat aatatgaccc gctcgtttgc ccgcaagctg
    26401 gcaccggaag tgaaagtgaa ttctattgcg ccatcgctga tcctgtttaa tgaacatgat
    26461 gatgccgaat atcgacaaca ggcgctgaat aaatcactga tgaaaaccgc tcctggcgag
    26521 aaagaagtga tcgacctggt cgattactta cttaccagtt gttttgtcac cggacgcagt
    26581 ttcccacttg atggcggtcg tcatctgcgt taatgcagct ttatccagca tataatcaac
    26641 acccatgcgc ttatccccca acacgcaaca gaaccaacaa aagcggcggg caagcgcatc
    26701 attccggtga aataccacag cgacaccagg taaacaaaat agggaataat cgaccacata
    26761 ctaaaaatga tggttgcgcg taaggcttca atgccgcgtt cgctggcaac aatataatgc
    26821 gcgataagcg caaaggtcgg gaaaagtgga atcagcccgg cgatataata atttttcgtt
    26881 tttgctaaaa caccaatcaa cagcactacc agcgcaccga gcgcggcttt gattaccagc
    26941 cccatttttt taccttaaca cttccataac aagtcatcag tagaatacct gatgaaaact
    27001 tgtttagaaa cgattgatag taagtaaaaa cagcgcggtg tattgtgacg tttttatatc
    27061 taccgtgaat gttatgaaca ctatcgtatt tgtggaagat gatgcggaag tcggttcact
    27121 gattgccgcg tacctggcaa aacatgatat gcaggttacc gtagagccgc gcggcgacca
    27181 ggccgaagaa accattttgc gagaaaatcc ggatttggtg ttactcgaca tcatgctacc
    27241 aggcaaggac ggcatgacca tttgtcgtga tttacgcgca aagtggtctg gaccgattgt
    27301 tcttctaacc tctctcgata gcgatatgaa ccacatcctg gcactggaaa tgggtgcctg
    27361 cgactatatt ctcaaaacga cgccccctgc tgttttgcta gcgcgtttac gtttgcattt
    27421 gcgtcagaat gagcaagcca cactgagcaa aggtcttcag gaaacgtctc tgactcccta
    27481 caaagccctg aatttcggca cgttgaccat cgatcccatc aaccgcgtag tcaccctggc
    27541 taacactgaa atctcgctct cgacagctga tttcgaatta ttgtgggaat tagctaccca
    27601 tgccgggcaa atcatggacc gcgatgcatt gctgaaaaat ttacgcggcg tcagttatga
    27661 cggactggat cgtagcgtgg acgtggctat ttcgcggtta agaaaaaaac tgctcgataa
    27721 cgccgcagaa ccttatcgca ttaaaactgt gcgtaacaaa ggctatcttt ttgcgcctca
    27781 tgcatgggaa taagcgatga aaaaactgtt tatccagttt tacctgttat tgtttgtctg
    27841 cttccttgtg atgtctctgc tggttgggtt ggtgtacaaa tttaccgccg aacgcgcggg
    27901 caaacagtcg ctggatgatt tgatgaacag ttcgctgtat ctgatgcgca gcgaattgcg
    27961 tgagatcccc ccacacgact ggggtaagac gctgaaagag atggatttaa atctctcttt
    28021 cgatctgcgt gtcgagccat tgagtaaata ccatcttgat gatatttcca tgcaccgact
    28081 gcgtggcggc gaaattgtcg ccctggacga tcagtacacg tttttgcagc gtatcccgcg
    28141 cagccactac gtgctggcag ttggtcctgt tccttatctt tattatctcc atcagatgcg
    28201 attgctggat atcgccctga tcgcttttat tgctatttcc ctcgcctttc cggtgtttat
    28261 ctggatgcgt ccgcactggc aggatatgtt aaaactggaa gcagcggcgc aacgatttgg
    28321 cgatgggcat ctcaatgagc gtatccactt tgatgagggt tcgagctttg aacgacttgg
    28381 tatcgcattt aaccagatgg cggacaatat caacgccttg attgccagca aaaaacagct
    28441 tattgacggt atcgctcacg aactgcgaac accgttagtg cgcctgcgtt atcgactgga
    28501 gatgagcgat aacctgagcg ccgccgaatc ccaggcgttg aatcgtgata tcagtcaact
    28561 tgaagcttta attgaagagc tgctgactta tgcccgactc gatcgcccac aaaacgagct
    28621 tcatcttagc gaaccagacc tgccgttgtg gctgtcaacg catctggcag acattcaggc
    28681 agtaacgccc gataaaacgg tacggattaa aacgctcgcg caaggccatt atgcggcgtt
    28741 ggatatgcgc ttaatggagc gcgtgctgga taatttgctc aataacgccc tgcggtactg
    28801 ccattcaacg gttgaaacca gcctgctact gtcggggaat agagcgacat taattgttga
    28861 ggatgatggc ccagggattg ccccagaaaa ccgcgaacat atctttgaac cttttgttcg
    28921 cctcgatccc agccgggatc gctcaaccgg cggctgcggg ctggggctgg caattgtcca
    28981 ctctatagca ctggcaatgg gcggtacggt taattgtgac accagcgaac tgggtggtgc
    29041 tcgcttctcg tttagctggc cgttatggca taacatcccg caatttacct ctgcctgaca
    29101 ctacgcgcac gatggtcaag tcaccacgac tgtgctataa aataagtatg ttgtaactaa
    29161 agtggttaat attatggcgc gttacgatct cgtagaccga ctcaacacta cctttcgcca
    29221 gatggaacaa gagctggcta catttgccgc tcatcttgag caacacaagc tattggttgc
    29281 ccgcgtgttc tctttgccgg aggtaaaaaa agaggatgag cataatccgc ttaatcgtat
    29341 tgaggtaaaa caacatctcg gcaacgacgc gcagtcgctg gcgttgcgtc atttccgcca
    29401 tttatttatt caacaacagt ccgaaaatcg cagcagcaag gccgctgtcc gtctgcctgg
    29461 cgtgttgtgt taccaggtcg ataacctttc gcaagcagcg ttggtcagtc atattcagca
    29521 catcaataaa ctcaagacca cgttcgagca tatcgtcacg gttgaatcag aactccccac
    29581 cgcggcacgt tttgaatggg tgcatcgtca tttgccgggg ctgatcaccc ttaatgctta
    29641 ccgcacgctc accgttctgc acgaccccgc cactttacgc tttggttggg ctaataaaca
    29701 tatcattaag aatttgcatc gtgatgaagt cctggcacag ctggaaaaaa gcctgaaatc
    29761 accacgcagt gtcgcaccgt ggacgcgcga ggagtggcaa agaaaactgg agcgagagta
    29821 tcaggatatc gctgccctgc cacagaacgc gaagttaaaa atcaaacgtc cggtgaaggt
    29881 gcagccgatt gcccgcgtct ggtacaaagg agatcaaaaa caagtccaac acgcctgccc
    29941 tacaccactg attgcactga ttaatcggga taatggcgcg ggcgtgccgg acgttggtga
    30001 gttgttaaat tacgatgccg acaatgtgca gcaccgttat aaaccacagg cgcagccgct
    30061 tcgtttgatc attccacggc tgcacctgta tgttgcagat taacgcccgg ctttcatact
    30121 gccgaccatc tgttctggcc gtacccagct gtcaaactcg gcttcgctaa gataccccag
    30181 cgcaagggcc gcagctttta aggtcagccc ttctttatgc gcttttttgg cgatctccgc
    30241 ggctttgtca taaccaatgt gggtgttaag cgcagtcacc agcatcagcg attcattgag
    30301 taattgattg attcgctcac gattcggttc aatacccact gcgcagtgtt tgttaaaact
    30361 ttccatgcca tctgccagca agcgcaccga ttgcaggaaa ttgtggatca ccattggacg
    30421 gaagacgttc agttcaaagt taccggaagc gccccccatg ttgatcgcca cgtcgttccc
    30481 catcacctga cagcagagca tggttaatgc ctcacactgt gttggattca ctttccccgg
    30541 catgattgag ctgcccggct cattttccgg gattgagatt tcaccaattc cgcagcgcgg
    30601 gccagaggcc agccagcgga catcattggc gattttcatc agtgacacag ccaacccttt
    30661 caacgcgccg tgcgcctgaa ccagggcatc acaggtcgcc agcgcttcaa atttgttcgg
    30721 cgcggtaaca aacggtgcac aggtaatgac tgccagttca tctgctacgc gacgcgcata
    30781 ctccggatgg gtatttagtc cagtacccac cgctgtaccg ccaagagcca gttccgctac
    30841 gtgaggcagg ctgtattcga tatgtttgag attatgctcg agcatcgcta cccagccgga
    30901 aatctcctgc cctagtgtta gcggcgtggc gtcctgcaag tgggttcgac cgattttgac
    30961 gatatcggca aatgcacgcg atttttcact cagtgtctgt gtcagggttt taagctgcgg
    31021 aatgagttgc ttgcgcagcg ccagcagcgc cgcaacgtgc atcgccgtcg gaaagacatc
    31081 gttggaactt tggcttttgt tcacgtcgtc gttagggtga actttacgtt ccatcccgcg
    31141 cacgccgccg agtaattcac tggcccggtt agccagcact tcgttcatgt tcatgttact
    31201 ttgcgtgccg gagccggtct gccagatagc cagcgggaat tcgtcgtcat gctgtcctgc
    31261 cagtacttca tccgccgcct gccgaatggc gctcgctttc tcttcagaca acaagcctaa
    31321 atcttcatta acttttgccg ctgcgcgctt ggttagcgcc agcgcatgaa tcagtgaggt
    31381 gggcattttc tccgtcgaaa tgcggaaatg ctccagcgag cgttgagttt gtgcgcccca
    31441 cagcttatct gccgggacat caatcgcccc catcgaatct ttttcgctgc gtactgtatt
    31501 catggcctgc tcctcacctg tttaattttt tctttctgtt ttgctttcgt taagcaactt
    31561 ttagctcact tattatttac tatttgataa caaatgtttg gtttttcgtg ccatgtaaaa
    31621 aaaccgcccc gaagggcggc tctgtttatt tcacacagcg agtgcactgt gtgagttgta
    31681 tctgctggaa gaagtcattt cctttatcat ccacaaggat aaacgccggg aaatcttcca
    31741 cttcaatttt ccagatggct tccattccca gttccggata ttcaacacat tccaggctct
    31801 taatacttcc ctgcgccaat acagcggccg gaccaccgat actgccaagg tagaagccgc
    31861 cgtgtttttt acaggcatcc gtcacctgct ggctgcggtt gcctttcgcc agcatgatca
    31921 tacttccgcc ctgcgcttgc agttgatcga cataagaatc catccgtccg gcggtcgttg
    31981 ggccaagaga accggaggca taaccttccg gcgttttggc cggacccgcg tagtaaatcg
    32041 gatgatcttt aatgtactgc ggcagccctt caccgttatc catccgctct ttcagtttgg
    32101 cgtgagcaat atcacgaccg acgataatcg tgccgttaag cgataagcgt gtagaaacgg
    32161 gatactgcga caactgtgcg aggatctctt tcatcggacg gttaaggtca acgcgcaccg
    32221 cttcgccttc tcccgcttta cgcagctctt cagggatata tttacctgga ttatgttcca
    32281 gtttttcgat ccagatcccc tgacggttga tcttcgcttt gatattacgg tcagcagagc
    32341 aggaaacacc cataccaacc ggacaggatg cgccgtgacg tggcaggcga atcacgcgta
    32401 tgtcgtgagc gaagtattta ccaccaaact gcgcacccag accgagattt tgcgcttcga
    32461 tcagcaattc tttttccagt tccacatcgc ggaatgcctg accgtgctca ttcccttccg
    32521 ttggcagttc gtcatagtat ttagcggaag ccagtttcac tgttttaagg ttagtttctg
    32581 cagaagttcc accaattacg aacgcaatat gatacggagg acaggctgcc gtacccagcg
    32641 tgcgcatctt ctcaaccagg taatttttca gtttccccgg cgttagtaac gctttggttt
    32701 cctgatagag atacgtcttg tttgccgaac caccaccttt ggcgatacag aggaatttgt
    32761 actcgtcgcc atcaacggca taaagatcga tctgcgctgg cagattggtg ccggtattca
    32821 cctctttata catatccagc ggcgcgtttt gcgagtagcg caaattatct tcgatataag
    32881 tgttatagac accgcgcgcc agcgccgctt catcacagcc accggtccat acacgctgcc
    32941 cttttttacc aacaataatc gcagtgccgg tatcctgaca ggttggcaga acacctttcg
    33001 ccgcgatgtc ggagttgcgc aggaattgca gcgccacata tttatcattt tcgctggctt
    33061 ccgggtcacg cagaatgtcg gccacctgtt gttggtgagc cggacgcagc ataaatgacg
    33121 catcatgaaa tgcctgacgc gccaacagag ttaacgcttc gggtgcgact ttcaaaatct
    33181 cctgcccttc aaattcagat acgctaacgt gttcgctggt tagcaggtaa tactcagtat
    33241 catctttttt gagtggaaaa ggagcctgat aatgaaaggg tttgtttgac attgttctct
    33301 cacttactgc ctggtttggt tatgctctgg gcgggtgttc cgttgccctg ttaaaagcga
    33361 gtaacaatat cctacacact tttttaacaa aaactgagac tagtacgact ttttgcggct
    33421 ccaggttact tcccgtagga ttcttgcttt aatagcggga ttaatttcca cattaaaaca
    33481 gggattgatc atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gcagcaaaac
    33541 ggcgttgact gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg
    33601 gatgggcgca catccgaaaa gcagttcacg ggtgcagaat gccgccggag atatcgtttc
    33661 actgcgtgat gtgattgaga gtaataaatc gactctgctc ggagaggccg ttgccaaacg
    33721 ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca gcacagccgc tctccattca
    33781 ggttcatcca aacaaacgca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat
    33841 cccgatggat gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt
    33901 tgcgctgacg cctttccttg cgatgaacgc gtttcgtgag ttttccgaga ttgtctccct
    33961 actccagcca gtcgcaggtg cacatccggc aattgctcac tttttacaac agcctaatgc
    34021 cgaacgttta agcgaactgt tcgccagcct gttgaatatg cagggtgaag aaaaatcccg
    34081 cgcgctggcg attttaaaat cggcgctcga cagtcagcag ggtgaaccgt ggcaaacgat
    34141 tcgtttaatt tctgaatttt acccggaaga cagcggcctg ttctccccgc tgttactgaa
    34201 tgtggtgaaa ttgaatcctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta
    34261 cctgcaaggc gtggcgctgg aagtgatggc gaactccgat aacgtgctgc gtgcgggtct
    34321 gacgcctaag tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc
    34381 ggctaaccag ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc
    34441 agtggatgat tttgccttct cgctgcatga ccttagtgat aaagaaacca ccattagcca
    34501 gcagagtgcc gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca
    34561 gcagttacag cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac
    34621 tgtcaaaggc cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa
    34681 aattaacatc tcttgctaag cttattaaag gcttataaca ccttcaggcg gccagtccgc
    34741 ctgatttcat tttatggata atcattatga ataaatcgct ggtagcggta ggcgtcattg
    34801 ttgcgctagg cgtagtctgg acaggcggcg catggtatac aggcaagaag attgaaaccc
    34861 atctcgaaga catggtcgcg caggcgaacg cgcaactcaa actgacagct cctgaatcca
    34921 acctggaagt gagttatcaa aactatcatc gcggcgtatt cagcagccag ttgcaactgt
    34981 tggtgaaacc cattgccggg aaagaaaatc cgtggattaa aagcggtcag agcgtcatct
    35041 tcaacgaatc ggttgatcat ggtcccttcc cgcttgccca gcttaaaaaa ctgaacctga
    35101 tcccgtcgat ggcatcaatt caaaccacgc tggttaataa cgaagtaagc aaaccactgt
    35161 ttgatatggc aaaaggtgaa acgccttttg agattaactc gcgcattggt tacagcggtg
    35221 attccagttc cgatatttcg ctcaagccac tgaattacga gcaaaaggat gaaaaagtcg
    35281 cctttagcgg cggcgagttc cagttaaatg cggacagaga cggcaaagct atctcccttt
    35341 ccggggaggc gcaaagtggt cggatagacg cagttaacga atacaaccag aaagtgcagt
    35401 tgacctttaa taatctgaaa accgacggtt ccagcacgct ggcaagtttt ggtgagcgcg
    35461 taggaaacca aaaactgtca ctggaaaaaa tgaccatttc ggtggaaggc aaagaactgg
    35521 cactgctgga aggcatggag atcagcggta aatcggatct ggtcaatga
//
LOCUS       DAFATA010000040        35544 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFATA010000040 DAFATA010000000
VERSION     DAFATA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.040"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..393)
                     /locus_tag="LGJ20_002349"
     CDS             complement(<1..393)
                     /locus_tag="LGJ20_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBK2386403.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMTAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGG"
     gene            complement(458..577)
                     /locus_tag="LGJ20_002350"
     CDS             complement(458..577)
                     /locus_tag="LGJ20_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK2386404.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(732..950)
                     /locus_tag="LGJ20_002351"
     CDS             complement(732..950)
                     /locus_tag="LGJ20_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2386405.1"
                     /translation="MKKLVRTLSAVDARVVCKPNRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKTICDLKVIV"
     gene            complement(1305..2678)
                     /locus_tag="LGJ20_002352"
                     /pseudo
     CDS             complement(1305..2678)
                     /locus_tag="LGJ20_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
     gene            complement(3429..3504)
                     /locus_tag="LGJ20_002353"
     tRNA            complement(3429..3504)
                     /locus_tag="LGJ20_002353"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3469..3471),aa:Ile,seq:cat)
     gene            4064..6316
                     /locus_tag="LGJ20_002354"
                     /pseudo
     CDS             4064..6316
                     /locus_tag="LGJ20_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6652..7629
                     /gene="csiD"
                     /locus_tag="LGJ20_002355"
     CDS             6652..7629
                     /gene="csiD"
                     /locus_tag="LGJ20_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBK2386406.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            7649..8917
                     /gene="lhgO"
                     /locus_tag="LGJ20_002356"
     CDS             7649..8917
                     /gene="lhgO"
                     /locus_tag="LGJ20_002356"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBK2386407.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERL
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            8940..10388
                     /gene="gabD"
                     /locus_tag="LGJ20_002357"
     CDS             8940..10388
                     /gene="gabD"
                     /locus_tag="LGJ20_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBK2386408.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            10402..11682
                     /gene="gabT"
                     /locus_tag="LGJ20_002358"
     CDS             10402..11682
                     /gene="gabT"
                     /locus_tag="LGJ20_002358"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBK2386409.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAGIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIINQCFAEAKQ"
     gene            11919..13319
                     /gene="gabP"
                     /locus_tag="LGJ20_002359"
     CDS             11919..13319
                     /gene="gabP"
                     /locus_tag="LGJ20_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBK2386410.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            13340..14002
                     /gene="csiR"
                     /locus_tag="LGJ20_002360"
     CDS             13340..14002
                     /gene="csiR"
                     /locus_tag="LGJ20_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBK2386411.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(14003..14452)
                     /gene="kbp"
                     /locus_tag="LGJ20_002361"
     CDS             complement(14003..14452)
                     /gene="kbp"
                     /locus_tag="LGJ20_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBK2386412.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(14536..14694)
                     /locus_tag="LGJ20_002362"
     CDS             complement(14536..14694)
                     /locus_tag="LGJ20_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBK2386413.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            14877..15176
                     /locus_tag="LGJ20_002363"
     CDS             14877..15176
                     /locus_tag="LGJ20_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2386414.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            15186..15710
                     /gene="ygaP"
                     /locus_tag="LGJ20_002364"
     CDS             15186..15710
                     /gene="ygaP"
                     /locus_tag="LGJ20_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBK2386415.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLIAGISGFCG
                     MARLLDKMPWNQQA"
     gene            complement(15757..16161)
                     /gene="stpA"
                     /locus_tag="LGJ20_002365"
     CDS             complement(15757..16161)
                     /gene="stpA"
                     /locus_tag="LGJ20_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBK2386416.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            16829..17278
                     /gene="alaE"
                     /locus_tag="LGJ20_002366"
     CDS             16829..17278
                     /gene="alaE"
                     /locus_tag="LGJ20_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBK2386417.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(17315..17659)
                     /locus_tag="LGJ20_002367"
     CDS             complement(17315..17659)
                     /locus_tag="LGJ20_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBK2386418.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            17811..18140
                     /locus_tag="LGJ20_002368"
     CDS             17811..18140
                     /locus_tag="LGJ20_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBK2386419.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            18388..18633
                     /gene="nrdH"
                     /locus_tag="LGJ20_002369"
     CDS             18388..18633
                     /gene="nrdH"
                     /locus_tag="LGJ20_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK2386420.1"
                     /translation="MRITIYIRNDCVQCHATKRAMENRSFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            18630..19040
                     /gene="nrdI"
                     /locus_tag="LGJ20_002370"
     CDS             18630..19040
                     /gene="nrdI"
                     /locus_tag="LGJ20_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK2386421.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            19013..21157
                     /gene="nrdE"
                     /locus_tag="LGJ20_002371"
     CDS             19013..21157
                     /gene="nrdE"
                     /locus_tag="LGJ20_002371"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK2386422.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            21167..22126
                     /gene="nrdF"
                     /locus_tag="LGJ20_002372"
     CDS             21167..22126
                     /gene="nrdF"
                     /locus_tag="LGJ20_002372"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK2386423.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            22481..23683
                     /gene="proV"
                     /locus_tag="LGJ20_002373"
     CDS             22481..23683
                     /gene="proV"
                     /locus_tag="LGJ20_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBK2386424.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            23676..24740
                     /gene="proW"
                     /locus_tag="LGJ20_002374"
     CDS             23676..24740
                     /gene="proW"
                     /locus_tag="LGJ20_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBK2386425.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            24797..25789
                     /gene="proX"
                     /locus_tag="LGJ20_002375"
     CDS             24797..25789
                     /gene="proX"
                     /locus_tag="LGJ20_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBK2386426.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            26080..27264
                     /locus_tag="LGJ20_002376"
     CDS             26080..27264
                     /locus_tag="LGJ20_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2386427.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            27388..28125
                     /gene="ygaZ"
                     /locus_tag="LGJ20_002377"
     CDS             27388..28125
                     /gene="ygaZ"
                     /locus_tag="LGJ20_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBK2386428.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            28115..28450
                     /gene="ygaH"
                     /locus_tag="LGJ20_002378"
     CDS             28115..28450
                     /gene="ygaH"
                     /locus_tag="LGJ20_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBK2386429.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            28541..29071
                     /gene="emrR"
                     /locus_tag="LGJ20_002379"
     CDS             28541..29071
                     /gene="emrR"
                     /locus_tag="LGJ20_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBK2386430.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            29198..30370
                     /gene="emrA"
                     /locus_tag="LGJ20_002380"
     CDS             29198..30370
                     /gene="emrA"
                     /locus_tag="LGJ20_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBK2386431.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            30387..31925
                     /gene="emrB"
                     /locus_tag="LGJ20_002381"
     CDS             30387..31925
                     /gene="emrB"
                     /locus_tag="LGJ20_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBK2386432.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(31989..32504)
                     /gene="luxS"
                     /locus_tag="LGJ20_002382"
     CDS             complement(31989..32504)
                     /gene="luxS"
                     /locus_tag="LGJ20_002382"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK2386433.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(32654..34210)
                     /gene="gshA"
                     /locus_tag="LGJ20_002383"
     CDS             complement(32654..34210)
                     /gene="gshA"
                     /locus_tag="LGJ20_002383"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBK2386434.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(34283..34711)
                     /locus_tag="LGJ20_002384"
     CDS             complement(34283..34711)
                     /locus_tag="LGJ20_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2386435.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(34708..35274)
                     /gene="yqaB"
                     /locus_tag="LGJ20_002385"
     CDS             complement(34708..35274)
                     /gene="yqaB"
                     /locus_tag="LGJ20_002385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBK2386436.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         8945 a         8802 c         8633 g         9164 t
ORIGIN      
        1 accacctgta taaacgtcct gattgccact gtcgattatc gtaccggtgg aaatgccccc
       61 gtcatgaatt gtctgtcttc ctccgttaat ggttgtatta ttagcctgcc ccacaaaatt
      121 gttatgactt cctacatctt gatatccacc cgattcaata agacttccat tagatacccc
      181 gccatgaaca ttctgctggc catggttgat aatatgagtg ttatttgttg tacctcgttc
      241 atctactctt tggctgccat ctacagtctc atcgtttacc acaccaataa catcaggagt
      301 gaaggccgtc atcccaggcg gggcatatat caaggcagat atcaataagg aaagtactga
      361 gcggcgacaa taatagggac tggtcctgtt cataaatttc atcctctgaa aagtgaatac
      421 tgagtagcgt ttaagcgacc ttagctttgc tgcaacatca gcccacaggc accagaccag
      481 gggattcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc aaagacatca
      541 tgatgaaatg atgatattcc gcataagaat gaggcatttt ttaaacgcag tgcgctgaag
      601 tgtggttgga taaaaaagtc aatccattca ggaaatacgg gcgtattctt ttctttcgac
      661 aatgaggccg ttggcaaaat aaaatgattt acataatcgt ttctgatgaa tatcttctgc
      721 tcacataaaa atcacacaat aactttgaga tcgcagattg ttttactttt acagcattcg
      781 tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat accacgtttg
      841 cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg gttcggttta
      901 cagactaccc gtgcgtccac cgccgataat gtcctgacca gttttttcag tccggacctc
      961 ctgccacggt tttacgttga agtggtaacc ctgagcacgc agttcttcag tcaggcgtgg
     1021 tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac tttcaggcag ctaaggaaag
     1081 ttgaaccaga cattagatga aatatttcaa ccaattacag caccaattca gtcactgcca
     1141 gcccaccaaa taaatcaagg ggttacatga aaacgtagcc cctttttctt tggtagtgac
     1201 actaaaatgg atgtagtgtg aagaataatc ccgtttactc aatcaataat acatattgtt
     1261 tcaatctacg ttattatctc tttgtaaaaa ttgccattta ttaatcattg aaaactgctt
     1321 ttagaacttg atacaacggg actagtcaca acaggactat tctcaacggg atcatcctca
     1381 gaggaactat catcaaagtc atcatccata aataaaatat catcgaatgg tgccacgccc
     1441 gtgatgagtt ttattttatt attacgatca gtcaatactc cacttaaacc gttttcgctc
     1501 acaggtttta atgatttttc attactcttg ttgtaagcag gcgcattaaa aatacacgga
     1561 gtatcaacat caaacaatga cgttccccag ttcacatatt gaatatcata gttactgaag
     1621 ttctgttcag aaaagaagca ccccttaaaa tccaatccac gtaaattata taaaccaccc
     1681 tcttcttttt ggagagtaat gttaattttg gctatctccc ggacatcatc gccatttttg
     1741 tatttgaata ccgtttcaag atgttctcca gacagcttga cttcaggaaa taatttgaaa
     1801 tcaaagccta tattattatg tgtcaacgta gatgaacaaa aaatggaaaa agcttgcagt
     1861 gctgaattat agctatcgat tttatcttga ggctgagctc ttggtaaaaa cttatagcaa
     1921 cactcataaa aattaaacag aagctctgaa gaccttgtat ttttatcaaa taataccccc
     1981 ttgaactcat tcacaaacgc atcttttttt tcttgaatat ctatggggtg ttcctttgac
     2041 tctgacaaag atgaaatctc atctattttg ttttcatatg aattacgtga ttccatacag
     2101 acatttggcg gcgtttctaa aataacacta cgcgtactac ttggcttaag taaaccaaca
     2161 tgaaaatcac tttttcttat attatcgaaa aggttctatt catttctctt agcgcattca
     2221 aaaaactgat cggcattatt tttattcgat aattttttag tttcagaaaa cacattttca
     2281 ttgttttcca gctttagttt aatgagaaga ttttcccaga cctgcttgct taaacatatt
     2341 acgtcaggct caccagaact aactaactcg tgattattat taaagtgtac gttgaatacc
     2401 tttaagttat tttcaccaac ttcatattta atacgtttta attgttctcc agctcccata
     2461 atgacaaagg cgttgccttc ttgatatata cattcagaca tcattttttg taaagtttca
     2521 agagcaccgc gatacgtctc tgatgcagct tccttacaaa ttaattttaa aatactatga
     2581 gctagtagct ccaagtgttt agaagattta ttttcattat aacttccact acccaaaact
     2641 gcgctagcgt taaatccgtt gcttttacta actaacattg tctattcctc aattaatgtc
     2701 tacatggcta tttttaatgt tattactgtt tgtcactata aaaaaacgct catttgagaa
     2761 aattactgat attaactgct tcacttgcta cgcatggaac ttttaattaa attagcacag
     2821 gaatgttaaa tttaataaac aaaaggttat ttcgctgtat gataaaaaaa accgttataa
     2881 tttattagtg aaaatcgttt ttcaagtgtt agaaatttat atctcaatag cgttggttaa
     2941 tgagcatagc cacgctcctg taacgctcac aaaactcatc tgcctgcggc gggtgttctg
     3001 gtcagtagta gatgtttaag gcgtggcaga gacatttcat ccttactcta cggcattgtt
     3061 ctacatacat tggttgtggt actcactcat catcagtgag cgaacagaga atagttcagt
     3121 gatttgagta attaacctga ttaaattaag gggtataata aatgataata ctctggcttt
     3181 atcgttaatt acttaattcc acatgtaagc aatttgcccg cttggcatag caggcatttt
     3241 ttccaggtac ttttgaatga gtactgatgg ataaatacat tgcagtggcg tgccacgtac
     3301 caaaacacca gccctcattc gaaaccaccc accgcacttc ttccttgaaa tggcgttagt
     3361 catgaaatat agaccgccat cgagtacccc ttgtaccctt aactcttcct gatacgtaaa
     3421 taatgatttg gtggcccttg ctggacttga accagcgacc aagcgattat gagtcgcctg
     3481 ctctaaccac tgagctaaag ggccttgagt gtgcaataac aatacttata aaccacgcaa
     3541 taaacatgat gatcatatga tgtaaataac agatttttat gcgttctcat tcatccttgc
     3601 tcgtcattac acgcgacata aaacccgaca ccgcttccat tcgcaaagtc gatactcgca
     3661 gtcaacaagc aaatgttaat aattagcact atctatagtt atcatcgatt caatgatact
     3721 ttgtaatgat tttgtatcta ataatataag tttattacat taactgaaga gttttcgcat
     3781 cattatgata tctgttactt ttcactccat aaaaataaac ttcgtatagc aatatattct
     3841 ttcatagatc ttattctgct aatcattagt ttcgtatgag cgatttttga cagttgcctc
     3901 tccagaccac atcgataatt aataaaacag atttaagcat tatccttttc catataaata
     3961 ttggataaaa gtaggacatc tgtttgcaat tactttcaca acaattaaac atttttatgt
     4021 ttccgtatac atcatattac tctaccatta gaggaacttt attatgtttt ctataaaacc
     4081 aggacccaga aatttaccta tcgacaaccc cacattgtta tcatggaaca ttactgacgg
     4141 ggatctaaat tccaaattaa atacattaga atatctaaac tgtataacaa atattattaa
     4201 ttcttgtgga gtttaccctc aaggattaaa agacagagaa attatatcaa cttttcacgc
     4261 agaaaaagtt attaatgatc tgttaaaaaa cgattataaa atttcccttt ctccagatac
     4321 aacttatcgt gagttgaata aagcagcaca gcgtagcatt acagcgccag acaggatagg
     4381 agaaggaaaa tcatgggttt atcaaagaga tacaatgatt gaaagaggtg ataacagcgg
     4441 tgttcatcag tatggtcctg ctgaacattt cacccacatt atatctgaca aaccgtcccc
     4501 aaaagatgag tatattgcat atgctattaa cattcctgac tatgagctgg cagccgatgt
     4561 atataatatt aacgtgacgt caccttccga acagcaagaa acatttaaaa tactaatcaa
     4621 tccagaacat ctacggcaaa cacttgagcg taaatctctt actgctgttc agaaatcaca
     4681 atgtgaaatc ataaccccca aaaaacctgg cgaagcgatt cttcatgctt ttaatgccac
     4741 ctaccagcaa atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca
     4801 aatccctcca gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaggg
     4861 ttactggttt catgcttatc aacccgaaga tctttgtacc atccataatc caatgggaga
     4921 tttgcaggat tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc
     4981 tgattatacc tttaacttta tgggaattgg gggtagtggt aaaaatgacc tggattatcc
     5041 ctctgctgat atacgagcga agatcagtaa agatatagaa ggtggtatcc ctggctattg
     5101 gcaaggtcag gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat
     5161 ccatccagaa gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatatc
     5221 taaggatgaa tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa
     5281 aacaacaccc aaaagtgacc aggttattat gttgcaaaat caatacgttc gtgaaatgcg
     5341 aaaatatggc gtacgaggtt tacgttacga tgcagcaaaa cactcaaaac atgaacaaat
     5401 agaaagatca ataactccac cgcttaaaaa ttataatgag cggttacaca atactaactt
     5461 atttaaccca aaatatcata aaaaagccgt tatgaattac atggaatatc tggtaacttg
     5521 tcagttggat gaacaacaaa tgtcatcact gctttatgaa agagatgatt taagcgccat
     5581 tgatttttca ttgctcatga aaacgataaa agccttttca tttggtggag atcttcaaac
     5641 ccttgcatca aaaccggatt ccacaatctc aagtatccca tcagaaagac ggatattgat
     5701 taacattaac cacgattttc ctaacaatgg taatcttttc aatgactttc tatttaacca
     5761 tcaacaagat gaacaattag caatggcata tatagccgct ctgccgttca gcaggccttt
     5821 agtttactgg gatggccaag tattaaaatc aacgactgaa attaaaaatt atgatgggtc
     5881 cacgcgtgtc ggcggtgagg cgtagcttaa taaaggttgc tctacctatc agcagctcta
     5941 caatgaattc cacgcattat atatagataa agcaggaata tggagcgcat ttgagggtgt
     6001 atctgcaact aaaaacgttc tggcctttag tcgtggggat tctgtgaaca ttaatcactc
     6061 tcctcatgat ggactagtta taataaataa aggaaacgaa gaagttgaag gtacctggcc
     6121 taacaaattg caacctggaa tatacaaaaa catggggagt aatagcgtta acattattat
     6181 taataatacc cgaaaaatta tcccccctgg taaagtattt acgcttagag gcggaactct
     6241 aaatatcaat attcctgggc gtagcgctct tcttttaggg aaaacaggag aaccgccgaa
     6301 ctatctctat ttataattta atgttatatc tgccccgata aaacggggca gataatatgt
     6361 ttagtttact aacggtcatt ttgcagtgaa gccatttact gttttttatc gaccagataa
     6421 tctgttctct aatgttaact ccccctaacc tgttgcttta gttattcatt tcctgtctca
     6481 ctttgcctta ataccctacg ttaaatgtta ctaatttgtt gcttttgatc acaataagaa
     6541 aacaatatgt cgcttttgtg cgcatttttc agaaatgtag atatttttag attatggcta
     6601 cgaaatgagc atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca
     6661 ctgaccgccg tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc
     6721 atcccgtcgg cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa
     6781 cagtttctcg aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg
     6841 cgttttcggg taggcaaaat tcttgacgat ctgtgtgcga atcagctgca accattgctg
     6901 ttgaagaccc tgctaaaccg cgctgaaggt gcgctgttga tcaatgcggt gggtatcgat
     6961 gatgtggcgc aggcagatga gatggtgaag ctggcaacgg cggtagcgca tctgattggt
     7021 cgctcaaatt tcgatgcgat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc
     7081 gataactcag acagctatct gcgtcagccg caccgcgtga tggaactgca caacgacggc
     7141 acgtatgtcg aagagatcac cgattacgtg ctgatgatga aaatcgatga gcaaaacatg
     7201 cagggtggaa attcgttgct gctgcatctc gatgactggg aacatctgga ccactatttc
     7261 cgccatccac tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc
     7321 aaagatgttt tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat
     7381 atcgaccagt tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc
     7441 gatgccattg aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg
     7501 ttgataaaca acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg
     7561 cgccgtgaac tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg
     7621 catcagtaag cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat
     7681 catcggcatg tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt
     7741 gctggaaaaa gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca
     7801 tgccggggtt tattacacgc ccggtagcct gaaggcgcag ttttgcctgg cgggaaaccg
     7861 cgccaccaaa gccttttgcg atcaaaacgg cattcgctat gacaactgcg gcaagatgct
     7921 ggtcgccacc tctgaactcg aaatggaacg gttgcgcgcg ttgtgggaac gcacggcggc
     7981 aaacggtatc gagcgcgagt ggttaaacgc catggaactg cgcgagcgcg aaccgaatat
     8041 caccgggctt ggcggcattt ttgtgccatc cagcggcatt gtcagctacc gcaacgtgac
     8101 ggcggcaatg gcaaaaatct tccaggccag aggcggtgag attatctata acgccgaagt
     8161 cagcggcctc agtgagcata aaaacggcgt ggtgatacgt acccgccagg gcggcgaata
     8221 tgaagcatcg acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct
     8281 cggcctcgaa ccgggcttta ttatctgccc gttccgcggc gagtatttcc gccttgcgcc
     8341 ggagcataac cagattgtta accatctgat ttaccctatt cccgacccgg caatgccgtt
     8401 tttgggcgtg catctcaccc gaatgatcga tggcagcgtg accgtcgggc caaacgcggt
     8461 gctggctttt aaacgcgaag gctaccgcaa gcgcgacttc tcgtttagcg acacgctgga
     8521 aattttaggc tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg
     8581 cgagatgaaa aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc
     8641 ccggctttcg ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcggtatc
     8701 gccggatggc aagttgattg acgattttct gtttgtcacc accccgcgca cgatccacac
     8761 ctgcaatgcg ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag
     8821 caaggtacaa acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg
     8881 tagtgtggat gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa
     8941 tgaaacttaa cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg
     9001 acgccaacaa tggcgaggtc atcgacgtca ccaatccggc gaacggcgac aagctgggta
     9061 gcgtacccaa aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgctc
     9121 tgcccgcctg gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca
     9181 atttgatgat ggagcatcag gacgatttag cgcgtctgat gaccctcgaa cagggtaaac
     9241 cgctggctga agcgaaaggt gaaatcagct acgccgcctc ctttattgag tggtttgctg
     9301 aagaaggcaa acgcatttat ggcgacacca ttcccggtca tcaggccgat aaacgcctga
     9361 ttgttatcaa gcagccgatt ggcgttaccg ccgccatcac gccgtggaac ttcccggcgg
     9421 cgatgattac ccgtaaagcc ggtccggcgc tggcggcagg ctgcacgatg gtgctgaaac
     9481 ccgccagtca gacgccgttc tctgcgctgg cgctggcgga gctggcgatt cgcgcgggca
     9541 ttccggctgg ggtatttaac gtggtcaccg gttcggcggg cgcagtcggt aacgaactga
     9601 ccagcaaccc gctggtgcgc aaactgtcgt ttaccggttc gaccgaaatt ggccgccagt
     9661 taatggaaca atgcgcgaaa gacatcaaaa aagtgtcgct ggagctcggc ggcaacgcgc
     9721 cgtttatcgt ctttgacgat gccgacctcg acaaagccgt ggaaggcgcg ctggcctcga
     9781 aattccgcaa cgccgggcaa acctgcgtct gcgccaaccg tttatacgtg caggacggcg
     9841 tgtatgaccg ctttgccgaa aaattgcagc aggcggtgag caaactgcac atcggcgacg
     9901 ggctggataa aggcgtcacc atcgggccgc tgatcgatga aaaagcagta gcaaaagtgg
     9961 aagagcatat tgccgatgcg ctggagaaag gcgcgcgcgt ggtttgcggc ggtaaagcac
    10021 acgaacgtgg cggcaacttc ttccagccga ccattctggt ggacgttccg gccaacgcta
    10081 aagtgtcgaa agaagagacg ttcggccccc tcgccccgct gttccgtttt aaagatgaag
    10141 ccgatgtgat cgcgcaagcc aatgacaccg aatttggtct tgccgcctat ttctacgccc
    10201 gtgatttaag ccgcgtcttc cgcgtgggcg aagcgctgga gtacggcatc gtcggcatca
    10261 ataccgggat tatttccaat gaagtggccc cgttcggcgg catcaaagcc tcgggtctgg
    10321 gtcgtgaagg ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg
    10381 gtctttaact ggagaacgcg aatgagcagc aataaagagt taatgcagcg ccgcagtcag
    10441 gcagttcctc gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc
    10501 cgggtgtggg acgttgaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc
    10561 aataccgggc acctgcatcc gaaggtggtt gcggcggtgg aagcgcagtt gaaaaaactg
    10621 tcgcacacct gcttccaggt gctggcctac gagccgtatc tggagctgtg cgagattatg
    10681 aatcagaagg tgccaggcga tttcgccaag aaaacgctgc tggttacgac cggttccgaa
    10741 gcggtggaaa acgcggtgaa aatcgcccgc gccgccacaa aacgtagcgg caccatcgct
    10801 tttagcggcg cgtaccacgg gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat
    10861 ccgtactctg cgggcatggg gctgatgcct gggcacgttt atcgcgcgct ttatccttgc
    10921 ccgctgcacg gcatcagtga agatgacgct atcgccagca ttcaccgaat ttttaaaaat
    10981 gacgctgcgc cggaagatat cgccgccatc gtgattgaac cggttcaggg cgaaggcggt
    11041 ttctacgccg ctacgcctgc ttttatgcag cgtttacgcg cgctgtgtga cgagcacggg
    11101 atcatgctga ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg
    11161 atggagcaga tgggcgttgc gccggatctc accacctttg cgaaatccat cgcgggcggc
    11221 ttcccgctgg cgggtgtcac cgggcgcgcg gaagtgatgg atgccgtcgc tccaggcggg
    11281 ctgggcggca cctatgccgg taacccgatt gcctgcgtgg cggcgctgga agtgttgaag
    11341 gtgttcgagc aggaaaatct gctgcaaaaa gccaacgatc tggggcagaa gctgaaagat
    11401 ggactgctgg cgattgccga aaaacacccg gagatcggcg acgtacgcgg gctgggagcg
    11461 atgatcgcca tcgagctgtt tgaagatggc gatcacagca aaccggacgc aaaactcacc
    11521 gccgggatcg tggcgcgcgc ccgcgataaa ggcctgattc ttctctcctg cggcccgtat
    11581 tacaacgtgc tgcgcatcct tgtaccgctc accattgaag acgctcagat ccgtcagggt
    11641 ctggagatca tcaaccagtg ttttgctgag gcgaagcagt agcaccgcac catgccggag
    11701 gcgacgctgc gcgtcttgtc cggcctacgg tgatccaggt cggataaggc gtttacgccg
    11761 catccgacaa tctgtacgtg aacaggaata aatctatgtc ggccggataa ggcggagccg
    11821 ctctccggca aaaagaatca ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg
    11881 gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac
    11941 atgagttagg cggcgggctg aaatcacgcc acgtcaccat gttgtctatt gccggtgtta
    12001 tcggcgcaag tctgtttgtc ggttcaagcg tcgccatcgc cgaagcgggc ccggcggtat
    12061 tactggccta tctgttcgcc ggattactgg tggttatgat tatgcggatg ttggcggaaa
    12121 tggcagttgc cacgcccgat accggttcgt tttccaccta tgccgacaag gcaattggcc
    12181 gctgggcggg ttataccatc ggctggctgt actggtggtt ttgggtactg gttatcccgc
    12241 tggaagccaa catcgccgct atgatcctgc actcgtgggt tccaggcatt cccatctggt
    12301 tattttccct cgtcattacc ctcgccttaa ctggcagtaa tttattaagc gttaaaaact
    12361 acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg gcctttattt
    12421 tccttggtgc agtcgcaatt agcggctttt acccttatgc cgaagtgagc gggatctcaa
    12481 gattgtggga tagcggcggc tttatgccca acggtttcgg tgcggtatta agcgcgatgt
    12541 tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatccg
    12601 acacgccgga aaaacatatt gtccgcgcca ccaactcggt tatctggcgt atttctatct
    12661 tctatttgtg ctctattttt gtcgtagtgg cgttaatacc gtggaatatg ccggggctga
    12721 aagccgttgg ttcttatcgc tcggtactgg aattgctcaa tattccccat gcgaaattaa
    12781 tcatggactg cgtgatatta ctttccgtaa ccagttgcct gaactcggcg ctgtataccg
    12841 cctcaaggat gctctactcc ttaagccgtc gcggagatgc gcctgcggta atgggtaaaa
    12901 tcaaccgcag taaaaccccg tacgtggcgg tgttactctc caccggcgcg gcattcttaa
    12961 cggtggtggt gaactattac gcgcctgcga aggtatttaa atttctgatc gacagctccg
    13021 gcgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgcgg atgcgcaaaa
    13081 ttctgcgagc agaaggaagc gaaattcgct tgcgaatgtg gctttatccg tggctcacct
    13141 ggctggtcat cggctttatt acctttgtgt tggtagtgat gctattccgt ccggcgcaac
    13201 agttagaagt gatctccacc ggcttattag cgatagggat tatctgtacc gtgccgatta
    13261 tggcgcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat acgcgctgat
    13321 aatgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct ggctgaagaa
    13381 cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta cgaatgagtt tgctgacatc
    13441 gcgttatgca cttggcgttg ggccgttacg ggaagctctt tcgcaactgg tggcggaacg
    13501 gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaggagct
    13561 gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ctggtcagtc tggcgattgc
    13621 ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc tgagtaagct
    13681 ggaggcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt
    13741 tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt
    13801 gtttgatctg gcggcgcgct atcgatttat ctggctgcgg cgaacggtgc tttcggtaga
    13861 aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac tggcgcgaga
    13921 taccgcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca
    13981 gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc cccggataaa
    14041 ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag
    14101 cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa
    14161 actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg gcaataccgg
    14221 aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg ccgtcaccag
    14281 tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg
    14341 ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa
    14401 ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct
    14461 cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg
    14521 ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat
    14581 atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag
    14641 cagcacgccg agaggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac
    14701 tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt
    14761 ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg ctgaggctgg
    14821 tatatgttgc atttttagtt tagaatttac ttaatttaga aaatacttaa ataaatatga
    14881 ctgaactcgc gcaattacag gccagtgccg aacaggcagc ggccttattg aaagcaatga
    14941 gccatcctaa acgattgctg attctgtgca tgcttagcgg ttcccccggc accagcgcgg
    15001 gagagctgac gcgcattacc ggactgagtg cctctgcgac atcacagcat ctcgctcgta
    15061 tgcgggacga agggcttatc gacagccaac gggatgccca acgcattcta tattccatta
    15121 aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag
    15181 tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg
    15241 caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt cctgaagcag
    15301 atctggctcc attatccgtg ctggaacagt caggtcttcc acctaaatta cgtcgcgagc
    15361 aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat aaattagcag
    15421 cgattgccgc ccccgcagaa atatttttac tcgaagatgg gattgatggc tggaaaagag
    15481 ctggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc caggtgcaga
    15541 tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta aatagcggtt
    15601 tcttcttatt aagtggcttt gttggtgccg ggttactgat tgcaggaatc agcggttttt
    15661 gtggaatggc aaggttgtta gataagatgc cgtggaacca acaagcttga gaagcgacgc
    15721 cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga
    15781 tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac cggtccaggt
    15841 tttagtttca ccgttaacat cagtgaattt atatttcgcc ggacgcggct ggcgtttttt
    15901 accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt taattccgtc
    15961 agctttcatc agctccagcc aggtgctaat tttttcctga cgctcagcca gttcacgctg
    16021 ctgctgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc
    16081 ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa tgttatttaa
    16141 actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagtatt
    16201 tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc tatttacagt
    16261 cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt tcaagattat
    16321 atattagcca ttttgatgag taatttagat tattggtgaa cagagtggcg ctctctgctc
    16381 gctaattccg caagcctgac aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa
    16441 gaaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca taaaaaatgc
    16501 tgtaaatgac acggtcacga catacaccga attctctacg cttatagcgt tacctcaccc
    16561 ccaaactttc attagtgaaa ttaaaggcaa ggtcatatct aattgattta attgaaaata
    16621 aattatatcg gcatgtaaaa atccacaatg tacaaaaaac gagccgttac ggaatatttt
    16681 atctacaaaa actgactaaa taaaaatttt tcactaattg aatagtcata gccagcgata
    16741 tacgctatgc gaaaatgcag atggcaatga gatccactgc tttcatctcc attaacatcc
    16801 cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc
    16861 agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt
    16921 tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt
    16981 gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc gcgcggcacg
    17041 caaagttagc ccgtcgggct ggataaaaaa tctggctgat atcctggctt atgtgacgtt
    17101 ccagtcaccg gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc atcagattat
    17161 ggcggcggtc agttcaaaca tcgttgtttc gatgttgatg ggggcggttt atggctactt
    17221 cctcgattat tgccgccgac tgtttaaagt cagccgttac cagcaggtaa aagcctgact
    17281 cttcttttcg cgactggcat cgccagtcgc gtctttaact ggcttcgcca aataaaccat
    17341 tcaaataacg ttcaagcgca acacgcgaac tgaagccatg cgggataccg taatgctcgt
    17401 gtcgctcgcc tgctaaatag agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg
    17461 aagcgggttc agccagtgtt gaactgcgtt cttttaatgt tggatgaatt accagagcgg
    17521 tacgtcccat tcgcgcttcg cgattaacat agacataatt ttccccacgg cggtaaccat
    17581 acgctttctg ggttaccaca tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct
    17641 cgtctggtcg taaatacata tttgatcctc gttattatca accgcgggcc taccttacct
    17701 gattgcgcat tcacaacgct ttcagaaaag tccataaagc cgcgatgaac ctcagttaag
    17761 aaaattatgg tctacactga aaattacatc gaattctaat gggggatcat atgtttaacc
    17821 gaccgaaccg caacgacgtt gatgatggcg tgcaggatat ccagaatgat gtcaatcaat
    17881 tagctgacag cctcgaatct gtattaaagt cttggggcag cgacgccaaa ggggaagctg
    17941 aagccgcacg cagtaaagct caggcattgc tgaaagaaac cagggcgcga atgcatggtc
    18001 gtactcgcgt ccagcaagcc gcacgcgatg ccgttggctg cgcggattct tttgttcgtg
    18061 aaagaccctg gtgtagcgtg ggtacagcag ctgcggtagg tatttttatc ggcgcactat
    18121 taagcatgcg caaatcgtag tgcaaaaatg ataataaata cgcgtctttg accccgaagc
    18181 ctgtcttcgg ggtttctttt tgcctggtga atcacaaaaa ttcccctacc ccgtcacgct
    18241 catatccagg gtaatttcga ccactatttg ctatatattg tgtggttgaa tcttttttca
    18301 actacatcta gtatctctgt atcaacagtg agacaacccg acgcgtatca tcgcgccgta
    18361 tcttcatttt aaacggaaat acgaatcatg cgcattacta tttacattcg taacgattgc
    18421 gttcagtgcc acgccaccaa acgggcgatg gaaaaccgga gctttgattt tgaaatgatt
    18481 aatgtcgatc gcgttcctga agcggcagaa gcgttgcgtg ctcagggctt tcgtcagttg
    18541 ccggtagtga ttgctggcga tcttagctgg tctggtttcc gtccggacat gattaaccgt
    18601 ctgcatccag cgccacacgc ggccagtgca tgagccagct cgtctacttc tccagcagct
    18661 ccgaaaatac gcagcgtttt atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca
    18721 atgagcggga acggattcag gtagacgagc cttacatcct gatcgtgccc tcttacggcg
    18781 gcggcggtac ggctggcgcg gtgccacgac aggtaattcg ctttttaaac gacgagcaca
    18841 accgggcgtt gcttcgcggc gttattgctt cgggtaatcg caactttggt gaggcgtatg
    18901 gccgtgccgg agatgtgatt gctcagaaat gcggcgtgcc gtggctgtac cgttttgaac
    18961 tcatgggtac gcaaagcgat atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac
    19021 gacaaccgca gaatgcctga cgcaggaaac gatggattac cacgcgctga atgcgatgct
    19081 taacctctac gatagcgcag gtcgcattca gttcgataaa gaccgccagg ccgttgacgc
    19141 ctttatggcg acgcatgtgc gtccgaacag tgtgaccttc agtagccagc agcagcgcct
    19201 gaactggctg gtcaacgaag gttactatga tgaaagcgtt cttaatcgct actctcgcga
    19261 ctttgtcatt acgctgtttg cccacgcaca caccagcggt tttcgtttcc agacattcct
    19321 cggggcatgg aagttttaca ccagctatac gttgaagaca ttcgacggta agcgttatct
    19381 ggaagatttt gccgatcgag tcacgatggt ggcgctaacg ctggcacaag gcgatgaaac
    19441 actggcgttg caactgaccg atgagatgct gtcaggacgc tttcagcctg caacaccgac
    19501 attcctcaac tgtggtaagc agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat
    19561 tgaagacaat atggagtcga ttggccgggc ggtaaattcc gcactgcaac tgtcgaaacg
    19621 cggcggcggc gtggcatttt tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg
    19681 tattgaaaat caatcttctg gcgtgattcc ggtgatgaaa atgctggaag acgcattttc
    19741 ctatgccaac cagctcggcg ctcgtcaggg ggctggtgca gtctatttac atgcccatca
    19801 tcccgatatt ctgcgttttc tcgatacgaa acgggaaaat gccgacgaga aaatccgcat
    19861 taaaacactg tcgctgggcg tggtgatccc ggatatcact ttccatctgg caaaagagaa
    19921 tgcgcagatg gcgctgtttt cgccttatga cgtagagcga gtttatggca agccgtttgc
    19981 cgatgtggcc atcagccaac actatgacga actggttgcc gatgaacgca ttcgcaaaaa
    20041 atacctcaac gcccgtgatt tcttccagcg actggcagaa atccagtttg agtccggcta
    20101 tccctacatc atgtatgaag acacggtaaa ccgtgctaac cctatcgccg ggcgcataaa
    20161 tatgagtaat ctctgctcag aaattttgca ggttaacagc gcctcagagt atgacgagaa
    20221 tctcgactat gcccgtacag gccatgatat ttcctgcaat ttaggttcgt tgaatattgc
    20281 ccacaccatg gattccccca actttgcccg cacggtagag actgccgtac gcggtttaac
    20341 ggcggtgtca gatatgagtc atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc
    20401 ctcacacgcc atcgggctgg ggcagatgaa tttacacggt tatctggcgc gagaaggcat
    20461 cgcttatggt tcgccggaag cactggattt caccaatctc tatttctata ccatcacctg
    20521 gcacgcactg cgtacctcga tgttgctggc acgcgaacgc ggtgaaacct tcgccgggtt
    20581 caaacagtca cgctatgcca gtggtgaata tttcagccaa tatctgcaag ggaactggca
    20641 gccgaaaacg gcgaaagttg gcgaactgtt tgcccgtagc ggtattacgt tacctacccg
    20701 tgagatgtgg gcgcaactgc gcgacgacgt gatgcgctac ggcatttata accagaatct
    20761 tcaggcggtg ccgccaaccg gttctatctc ttatatcaac catgctacgt cgagtattca
    20821 tccgattgtg gcgaaagtag agatacgcaa agagggcaaa acaggacgcg tttactaccc
    20881 tgccccgttt atgactaacg agaatctggc gctgtatcag gatgcttacg aaatcggcgc
    20941 agaaaagatc atcgacacct acgcggaagc gactcgccat gtcgatcagg gtttgtcgct
    21001 gacgctgttt ttccccgata ccgccaccac tcgcgatatc aacaaagcgc agatttacgc
    21061 ctggcgcaaa ggtatcaaaa cgctctatta cattcgcctg cgtcagatgg cgctggaagg
    21121 cactgaaatt gaaggctgcg tctcctgtgc actttaagga atatctatga aactctcacg
    21181 tatcagcgcc atcaactgga acaagatatc tgacgataaa gatctggagg tgtggaatcg
    21241 cctgaccagc aatttctggc taccagaaaa ggtgccgctg tcgaacgata ttcctgcctg
    21301 gcagacatta actgtcgtag aacaacaact gacgatgcgc gtttttactg gcctgacgct
    21361 gctcgacacg ctgcaaaatg ttatcggcgc gccttcgctg atgcccgatg cactcacgcc
    21421 tcatgaagaa gcggtattat cgaatatcag ctttatggaa gcggttcatg cccgctctta
    21481 cagttcgatt ttctcgacgc tgtgtcagac caaagatgtc gatgccgcct acgcctggag
    21541 tgaagaaaac gcaccgttgc agcgaaaagc tcagattatt cagcaacatt atcgcggtga
    21601 tgatccgctg aaaaagaaaa tcgccagtgt gtttcttgaa tcttttttgt tttattccgg
    21661 tttctggctg ccgatgtatt tttccagccg cggaaagctg accaataccg cagatctgat
    21721 ccgtctgatt atccgcgatg aagcagtcca cggttactac ataggctata aatatcagaa
    21781 aaacatggaa aagatatctc tgggacaacg tgaagagttg aagagtttcg ccttcgattt
    21841 gttgctggaa ctctacgaca acgagttgca atacaccgat gagctgtacg ccgaaacccc
    21901 gtgggctgac gatgtgaaag cgtttctctg ttacaacgcc aataaggctt tgatgaatct
    21961 gggctacgaa ccgttatttc ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc
    22021 gctttcgccg aatgccgatg aaaatcacga tttcttttcc ggttcaggct cctcttatgt
    22081 gatggggaaa gcggttgaaa cagaagatga agactggaat ttctgagggt gttattttca
    22141 aaaatatcac tacccgcagc agggaaataa ttcccgccaa atagcttttt atcacgcaaa
    22201 tattctgtag tgatctacac tgatactctg ttgcattatt cgcctgaaac tacaatattc
    22261 aggcgttttt tcgctatcta tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc
    22321 ttatatcacg ggaaattccg gcgatttgct cgcatcaata ttcatgccac atttgccctc
    22381 aggggttgcc tcagattctc agtatgttag ggtagaaaaa agtgactatt tccattgggt
    22441 aatatatcga cataaacaaa taaaggaatc tttctattgc atggcaatta aattagaaat
    22501 taaaaatctt tataaaatat ttggcgagca tccacagcga gcgttcaaat atatcgaaca
    22561 aggactttcg aaagaacaaa ttctggaaaa aactgggcta tcgcttggcg taaaagacgc
    22621 cagtctggcc attgaagaag gcgagatatt tgtcatcatg ggattatccg gctcgggtaa
    22681 atccacaatg gtacgccttc tcaatcgcct gattgaaccc acccgcgggc aagtgctgat
    22741 tgatggtgtg gatattgcca aaatatccga tgccgaactc cgtgaggtgc gcagaaaaaa
    22801 gattgcgatg gtcttccagt cctttgcctt aatgccgcat atgaccgtgc tggacaatac
    22861 tgcgttcggt atggaattgg ccggaattaa tgccgaagaa cgccgggaaa aagcccttga
    22921 tgcactgcgt caggtcgggc tggaaaatta tgcccacagc tacccggatg aactctctgg
    22981 cgggatgcgt caacgtgtgg gattagcccg cgcgttagcg attaatccgg atatattatt
    23041 aatggacgaa gccttctcgg cgctcgatcc attaattcgc accgagatgc aggatgagct
    23101 ggtaaaatta caggcgaaac atcagcgcac cattgtcttt atttcccacg atctcgatga
    23161 ggctatgcgt attggcgacc gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg
    23221 cacaccggat gaaattctca ataatccggc gaatgattat gtccgtacct tcttccgtgg
    23281 cgttgatatt agtcaggtat tcagtgcgaa agatattgcc cgccggacac cgaatggctt
    23341 aattcgtaaa acccctggct tcggcccacg ttcggcactg aaattattgc aggatgaaga
    23401 tcgcgaatat ggctacgtta tcgaacgcgg taataagttt gtcggcgcag tctccatcga
    23461 ttcgcttaaa accgcgttaa cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc
    23521 gttagcagtc gatgcacaaa cgcctcttag cgagttgctc tctcatgtcg gacaggcacc
    23581 ctgtgcggtg cccgtggtcg acgaggacca acagtatgtc ggcatcattt cgaaaggaat
    23641 gctgctgcgc gctttagatc gtgagggggt aaataatggc tgatcaaaat aatccgtggg
    23701 ataccacgcc agcggcggac agtgccgcgc aatccgcaga cgcctggggt acaccggcga
    23761 ctgcaccgac tgacggcggt ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg
    23821 agcattttaa tattctcgat ccgttccata aaacgctgat cccgctcgac agttgggtca
    23881 ctgaagggat cgactgggtt gttacccatt tccgtcccgt cttccagggc gtgcgcgttc
    23941 cggttgatta tatcctcaac ggtttccagc agttgctgct gggtatgccc gcaccggtgg
    24001 cgattatcgt tttcgctctc atcgcctggc agatttccgg ggtcggaatg ggcgtggcga
    24061 cgctggtttc gctgattgcc atcggcgcaa tcggtgcctg gtcgcaggca atggttactc
    24121 tggcgctggt gttaaccgcc ctgctgttct gtatcgtcat cggtttgccg ttggggatct
    24181 ggctggcgag aagtccgcga gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga
    24241 ccacgccagc gtttgtttat ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc
    24301 cgggcgtggt ggtgacgatc atctttgctc tgccgccgat tatccgtctg accattctgg
    24361 ggattaacca ggttccggcg gatctgattg aagcctcgcg ctcattcggt gccagcccgc
    24421 gccagatgct gttcaaagtt cagttaccgc tggcgatgcc gaccattatg gcgggcgtta
    24481 accagacgct gatgctggcc ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg
    24541 ggctgggtca gatggtactt cgcggtatcg gtcgtctgga tatggggctt gccaccgttg
    24601 gcggcgtcgg gattgtgatc ctcgccatta tcctcgaccg cctgacgcag gccgttgggc
    24661 gcgactcacg cagtcgcggc aaccgtcgct ggtacaccac tggccctgtc ggtctgctga
    24721 cccgcccatt cattaagtaa ctctgcactt gcccggtaac gccgggcata tcaccctgcc
    24781 aaaaaaagga ataacaatgc gacatagcgt actttttgcg acagcgtttg ccacgcttat
    24841 ctctacacaa acttttgctg ccgacctgcc gggcaaaggc attacagtta atccagttca
    24901 aagcaccatc accgaagaaa ccttccagac actgctggtc agtcgtgcgc tggagaaatt
    24961 aggttatacc gtcaataaac ccagcgaagt agattacaac gttggctaca cctcgcttgc
    25021 ttccggcgat gcaaccttca ccgccgtgaa ctggacgccg ctgcatgaca acatgtacga
    25081 agctgccggt ggcgataaga aattttatcg tgaaggggta tttgttaacg gcgcggcaca
    25141 gggttacctg atcgataaga aaaccgccga ccagtataaa atcaccaaca tcgcacaact
    25201 gaaagatccg aagatcgcca aactgttcga taccaacggc gacggtaaag cggatttaac
    25261 cggttgtaac cctggctggg gctgcgaagg tgccatcaac caccagcttg ccgcgtatga
    25321 actgaccaac accgtgacgc ataatcaggg gaactacgca gcgatgatgg ccgacaccat
    25381 cagtcgctac aaagagggca aaccggtgtt ttattacacc tggacgccgt actgggtgag
    25441 taatgagctg aagccaggga aagatgtggt ctggttgcag gtgccgttct ccgcactgcc
    25501 gggcgataaa aatgccgata ccaaactgcc gaatggcgcg aattacggct tcccggtcag
    25561 caccatgcat atcgttgcca acaaagcctg gaccgagaaa aatccggcgg cagccaaact
    25621 gtttgccatt atgcagttgc cagtggccga tattaacgcc cagaacgcca ttatgcatga
    25681 cggcaaagcc tcagaaggcg atattcaggg ccacgttgat ggctggatca aagcccacca
    25741 gcagcagttc gatggctggg tgaatgaggc gctggcagcg cagaagtaat ttttattcag
    25801 gcggataagg cgtttttgcc tcatccgccg ctctgtacac aatgcctgat gcgacgctga
    25861 cgcgtcttat catgcctaca aaatacactc attcgtatgg cggataaggc gttttcgccg
    25921 catccgccgt tctgtgcaca atgcctgatg cgacgctggc gcgtcttatc atgcctacaa
    25981 accacatcgc acatttcaac aatctatctt tcatcccata ttcatcaaca tccgctatta
    26041 ttgatttcca gcttaatcat cacctgatga acaaaaataa tgactaaacc taatcatgag
    26101 cttagcccgg cgctgatcgt gctgatgtct atcgccaccg gtctggcggt agccagtaac
    26161 tattacgccc agccattgct cgacaccatc gcgcgtaact tttccctttc cgccagttcg
    26221 gcaggcttta ttgttaccgc cgcgcagttg ggctatgccg caggtctact gtttcttgtt
    26281 cccctcggtg atatgtttga acgccgccgc ctgattgtct cgatgacctt actggcagcg
    26341 ggcggtatgt taatcactgc cagcagtcag tcactggcga tgatgatcct cggtacggca
    26401 ttaaccggtt tattctcagt cgtggcacaa attctggttc cgctggcagc gacgctggct
    26461 tcaccggaca aacgcggcaa agtggttggc actattatga gcggtctgct gttggggatc
    26521 ttactcgcac ggaccgttgc cggattgctg gcgaatctcg gcggctggcg caccgtcttt
    26581 tgggttgcct cggtgttaat ggcactgatg gcgctggcat tatggcgtgg tctgccacaa
    26641 atgaaatcag aaacccacct caactaccca cagttgttag gttccgtttt cagcatgttt
    26701 atcagcgata agatcctgcg cacccgcgca ttgctgggct gcctgacctt tgccaacttc
    26761 agcattctct ggacctcaat ggcctttttg cttgccgctc caccttttaa ctacagcgat
    26821 ggtgtcattg gtctgtttgg acttgcggga gctgccggag cgttgggcgc tcgtccggcg
    26881 ggcggttttg ccgataaggg taaatcgcac cacaccacaa ctttcggtct gctgctgcta
    26941 ttactttcat ggctggcgat ctggtttggt cacacttccg tactggcgct gattatcggc
    27001 atcctggtgc tggatctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat
    27061 cgaatacacc ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt
    27121 ggcggtgccg ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc
    27181 gtttgtctgg ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt
    27241 catcgtcagg aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata
    27301 agggtgttaa gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa
    27361 tattaacaat cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt
    27421 tcggcgacct tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg
    27481 gtggcctttg cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt
    27541 tttttctcct gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca
    27601 gccgggagta gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtg
    27661 ttgtatggcc cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc
    27721 ctgtgggcat ttggcctgac ggatgaggtt tttgccgccg caaccgcaaa actggtacgc
    27781 aataatcgcc gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg
    27841 tgggtatttg gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc
    27901 gccgttgaag ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc
    27961 tctttccagc gcaaacaatc tctttgcgtt accgcagcgt tagttggtgc ccttgcaggc
    28021 gtaacgctat tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact
    28081 gcgttaatcc aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg
    28141 gttactggtt ggcgtggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg
    28201 taatgcccgc ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc
    28261 ctcgatatgc gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg
    28321 tttcgtgccc acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag
    28381 cattatcatc ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc
    28441 gattatataa cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg
    28501 tcgttactat atcggctgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc
    28561 cattgaacaa atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat
    28621 ccttctgact cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat
    28681 gctgaaggct caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca
    28741 ggaaaaccac agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa
    28801 cgcgacgcgt attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga
    28861 taacgatcgc cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga
    28921 ggttttacca ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga
    28981 aaaagatcag ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca
    29041 agacggtgtg gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa atccagattt
    29101 ataaaagaaa aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg
    29161 gctcagccga tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca
    29221 aaccccgcag caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac
    29281 cttgctcttt ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca
    29341 tttcgaagaa accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt
    29401 gtctggcagc gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt
    29461 tctggtcact ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact
    29521 ggcttccagc gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa
    29581 tattgaggtg caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc
    29641 gctgggcaat gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac
    29701 cagtgcccag gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat
    29761 tctggggact aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa
    29821 cgcctggctg gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtctcccg
    29881 ccgcgcggta cagcctgggg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc
    29941 agccaccaat atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgtat
    30001 cggtcagccg gtcactatca ccacggatat ttacggcgat gatgtgaaat acaccggtaa
    30061 agtggttggt ctggatatgg gcacaggtag cgcattctca ctgcttccgg cgcaaaatgc
    30121 gaccggtaac tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa
    30181 acagctggag caatacccgc tgcgcattgg tttgtccacg ctggtgagcg tcaataccac
    30241 taaccgtgac ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac
    30301 cgcgcgtgaa atcagcctgg cacctgtcaa taaactgatc gacgatatcg taaaagctaa
    30361 cgctggctaa tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac
    30421 tggtcattat gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca
    30481 ttgctaacgt ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccagggaa
    30541 cgtgggtaat cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc
    30601 tggcaaagcg cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg
    30661 cgtcgtgggc gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc
    30721 aggggattgt cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc
    30781 cgccagccaa acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa
    30841 tttgcggccc gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct
    30901 tcatcaacgt gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac
    30961 gtgaaacccg caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg
    31021 gtatcggcag cctgcagatt atgctcgacc gcggtaaaga gctggactgg ttttcatcac
    31081 aggaaattat cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg
    31141 agctgaccga cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca
    31201 tcggctgctt gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc
    31261 cgcagttgtt gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc
    31321 cggtagggat tattccggtg atcctgtcgc cgattatcgg ccgcttcgcg cataaactgg
    31381 atatgcggcg gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg
    31441 cctatacctt tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg
    31501 ggtttgcggt ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac
    31561 cggaacgact ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta
    31621 tcggcacgtc gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt
    31681 tgactgagtc ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag
    31741 ggcttgggat gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg
    31801 ggctgattat ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc
    31861 tggggctggt gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc
    31921 actaagtaca actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag
    31981 tttactgact agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg
    32041 ctgttgatgc gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac
    32101 gagtgcatct ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc
    32161 tgcactttca gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct
    32221 ggcgtaccaa tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata
    32281 atctctacac cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg
    32341 gtatggatcc ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac
    32401 acggtgattg cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct
    32461 tccatccggg tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta
    32521 atttttttta aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg
    32581 cgcatttgtt atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc
    32641 tttttctttt ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg
    32701 tttccatttc ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc
    32761 gcagaatttc cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc
    32821 ctgttccgcc aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat
    32881 cgggattatc gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt
    32941 taatgctatc cagcgtttgc gcgacgcgtt tcagatcgcg gaacagatct ttacccacct
    33001 gcagtaacgg gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac
    33061 cttcaaggat cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt
    33121 ccggtgcatc agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt
    33181 catctacacc aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa
    33241 tgccgccacg taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac
    33301 gaatcggcgc atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct
    33361 taccgtcttt ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt
    33421 taaggcccgc tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt
    33481 tattggtata gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac
    33541 cgcactcggt tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa
    33601 tcgccggaga tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga
    33661 taacgcggaa atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc
    33721 acttcgcttg ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt
    33781 gcatcagcgc gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg
    33841 tgttagaagt gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca
    33901 tacttaacgg ccacatccgc tcatcaccca tattgcgcgc cgtataacga tgcagatcgc
    33961 gcataaaggt cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg
    34021 cttccgcaaa atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat
    34081 gacctgttgt tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac
    34141 gctgtatccc ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt
    34201 ccgggatcaa attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt
    34261 gaccatatgt gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg
    34321 caactgcaac atagcgtaac gctttaccaa ggcacaaaaa aaagattacc ggtccccagg
    34381 agatgcgcat ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta
    34441 ataatagcgt gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg
    34501 atgttttacg caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat
    34561 tacccattgt tgctgttaaa actaaaaccc agggataact gatcccggaa agcaacattg
    34621 ccaccagcac gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa
    34681 acaacgagaa aagcgataac gcttcactca cagcaagcga acatccacgg cgtccatgcc
    34741 tgctgcacgg gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg
    34801 ttgcacgccc atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt
    34861 gacgtgatca gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa
    34921 tgcctcagcg atggcgcttt cactccccgt tcctacagcc attgggcgac gaccatgcca
    34981 acttttcacc acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac
    35041 tgcttctgtt ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat
    35101 aattgcctga gcaatacgcc aggtgggcga tccattaagc gcaatcattg cctgaacatc
    35161 gtactgaaga ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt
    35221 atccaggatt gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt
    35281 ctcgcaagtc gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg
    35341 cgaaggagaa gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg
    35401 aaggaaattt ttgggagaag atggtgcatc cgggaggatt actcactggc gttcgccctt
    35461 cgggccgttg ctgacgcaac gttatcctcc ctggtgcctg cggttaatac cgctatctct
    35521 tcgatacctt cattgctgaa ggtt
//
LOCUS       DAFATA010000041        35323 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFATA010000041 DAFATA010000000
VERSION     DAFATA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.042"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(65..2494)
                     /gene="torZ"
                     /locus_tag="LGJ20_002386"
     CDS             complement(65..2494)
                     /gene="torZ"
                     /locus_tag="LGJ20_002386"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBK2386437.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(2519..3619)
                     /locus_tag="LGJ20_002387"
     CDS             complement(2519..3619)
                     /locus_tag="LGJ20_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBK2386438.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGCGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGAD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(4007..4753)
                     /gene="cutC"
                     /locus_tag="LGJ20_002388"
     CDS             complement(4007..4753)
                     /gene="cutC"
                     /locus_tag="LGJ20_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK2386439.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(4767..5333)
                     /locus_tag="LGJ20_002389"
     CDS             complement(4767..5333)
                     /locus_tag="LGJ20_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK2386440.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            5549..7282
                     /gene="argS"
                     /locus_tag="LGJ20_002390"
     CDS             5549..7282
                     /gene="argS"
                     /locus_tag="LGJ20_002390"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK2386441.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            7459..7947
                     /locus_tag="LGJ20_002391"
     CDS             7459..7947
                     /locus_tag="LGJ20_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBK2386442.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(8067..8459)
                     /gene="flhe"
                     /locus_tag="LGJ20_002392"
     CDS             complement(8067..8459)
                     /gene="flhe"
                     /locus_tag="LGJ20_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBK2386443.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(8459..10537)
                     /gene="flhA"
                     /locus_tag="LGJ20_002393"
     CDS             complement(8459..10537)
                     /gene="flhA"
                     /locus_tag="LGJ20_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBK2386444.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(10530..11678)
                     /gene="flhB"
                     /locus_tag="LGJ20_002394"
     CDS             complement(10530..11678)
                     /gene="flhB"
                     /locus_tag="LGJ20_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBK2386445.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FYLWHHWPQMMRLMAESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFLQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(11880..12524)
                     /gene="cheZ"
                     /locus_tag="LGJ20_002395"
     CDS             complement(11880..12524)
                     /gene="cheZ"
                     /locus_tag="LGJ20_002395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBK2386446.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(12535..12924)
                     /gene="cheY"
                     /locus_tag="LGJ20_002396"
     CDS             complement(12535..12924)
                     /gene="cheY"
                     /locus_tag="LGJ20_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBK2386447.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(12939..13988)
                     /gene="cheB"
                     /locus_tag="LGJ20_002397"
     CDS             complement(12939..13988)
                     /gene="cheB"
                     /locus_tag="LGJ20_002397"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBK2386448.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(13991..14851)
                     /gene="cheR"
                     /locus_tag="LGJ20_002398"
     CDS             complement(13991..14851)
                     /gene="cheR"
                     /locus_tag="LGJ20_002398"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBK2386449.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(14870..16471)
                     /gene="tap"
                     /locus_tag="LGJ20_002399"
     CDS             complement(14870..16471)
                     /gene="tap"
                     /locus_tag="LGJ20_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBK2386450.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESVQLQIAPVVS"
     gene            complement(16517..18178)
                     /gene="tar"
                     /locus_tag="LGJ20_002400"
     CDS             complement(16517..18178)
                     /gene="tar"
                     /locus_tag="LGJ20_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBK2386451.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYQFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(18323..18826)
                     /gene="cheW"
                     /locus_tag="LGJ20_002401"
     CDS             complement(18323..18826)
                     /gene="cheW"
                     /locus_tag="LGJ20_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBK2386452.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(18847..20811)
                     /gene="cheA"
                     /locus_tag="LGJ20_002402"
     CDS             complement(18847..20811)
                     /gene="cheA"
                     /locus_tag="LGJ20_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBK2386453.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(20816..21742)
                     /gene="motB"
                     /locus_tag="LGJ20_002403"
     CDS             complement(20816..21742)
                     /gene="motB"
                     /locus_tag="LGJ20_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBK2386454.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLWKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(21739..22626)
                     /gene="motA"
                     /locus_tag="LGJ20_002404"
     CDS             complement(21739..22626)
                     /gene="motA"
                     /locus_tag="LGJ20_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBK2386455.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(22753..23331)
                     /gene="flhC"
                     /locus_tag="LGJ20_002405"
     CDS             complement(22753..23331)
                     /gene="flhC"
                     /locus_tag="LGJ20_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBK2386456.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(23334..23684)
                     /gene="flhD"
                     /locus_tag="LGJ20_002406"
     CDS             complement(23334..23684)
                     /gene="flhD"
                     /locus_tag="LGJ20_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBK2386457.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            24464..24892
                     /gene="uspC"
                     /locus_tag="LGJ20_002407"
     CDS             24464..24892
                     /gene="uspC"
                     /locus_tag="LGJ20_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBK2386458.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(24899..26323)
                     /gene="otsA"
                     /locus_tag="LGJ20_002408"
     CDS             complement(24899..26323)
                     /gene="otsA"
                     /locus_tag="LGJ20_002408"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBK2386459.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(26298..27098)
                     /gene="otsB"
                     /locus_tag="LGJ20_002409"
     CDS             complement(26298..27098)
                     /gene="otsB"
                     /locus_tag="LGJ20_002409"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBK2386460.1"
                     /translation="MTEPLTEAPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(27265..28251)
                     /gene="araH"
                     /locus_tag="LGJ20_002410"
     CDS             complement(27265..28251)
                     /gene="araH"
                     /locus_tag="LGJ20_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBK2386461.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(28266..29780)
                     /gene="araG"
                     /locus_tag="LGJ20_002411"
     CDS             complement(28266..29780)
                     /gene="araG"
                     /locus_tag="LGJ20_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBK2386462.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(29850..30839)
                     /gene="araF"
                     /locus_tag="LGJ20_002412"
     CDS             complement(29850..30839)
                     /gene="araF"
                     /locus_tag="LGJ20_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBK2386463.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            31636..32139
                     /locus_tag="LGJ20_002413"
     CDS             31636..32139
                     /locus_tag="LGJ20_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBK2386464.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(32218..32469)
                     /locus_tag="LGJ20_002414"
     CDS             complement(32218..32469)
                     /locus_tag="LGJ20_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBK2386465.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(32584..32670)
                     /gene="azuC"
                     /locus_tag="LGJ20_002415"
     CDS             complement(32584..32670)
                     /gene="azuC"
                     /locus_tag="LGJ20_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBK2386466.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            32933..33256
                     /locus_tag="LGJ20_002416"
     CDS             32933..33256
                     /locus_tag="LGJ20_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBK2386467.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            33427..33924
                     /gene="ftnA"
                     /locus_tag="LGJ20_002417"
     CDS             33427..33924
                     /gene="ftnA"
                     /locus_tag="LGJ20_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBK2386468.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(33962..34201)
                     /locus_tag="LGJ20_002418"
     CDS             complement(33962..34201)
                     /locus_tag="LGJ20_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBK2386469.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            34392..>35323
                     /gene="tyrP"
                     /locus_tag="LGJ20_002419"
     CDS             34392..>35323
                     /gene="tyrP"
                     /locus_tag="LGJ20_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBK2386470.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGR"
BASE COUNT         8650 a         9390 c         8345 g         8938 t
ORIGIN      
        1 tagcgcatca ggcagtttta cgtttgcata acctcagcgc ccgtttccgg gcgctattca
       61 cttcttacgc ctgaacagca ggcttatcaa atgccgttaa cttcggtgcg ttacccgtat
      121 atttttcgat atacaccagc gcagagttac cggcacaggc atttgccagc tgcgagctgg
      181 ggatatccgc cgttaacacg ttcgcactgc cgtttttaca caagccgttt tccagatctg
      241 gccatgcacc ttcatgcacg cataccacgc cttttttgat cccgtcagtc accaccgcgc
      301 ctgtcaaaat ctgaccacgt ttgttccaga cacgcaccag atcgccattc gcaataccaa
      361 atcgagcagc atcttcggtg tgaatagtga tcggttcacg atcggcaacc gcatattttt
      421 tacgcagttc cgcatagtta agctgactat gtaaacggtg tgccggatgt gcggtcagaa
      481 gctgcaactg cttctcgtcg gcggtaccct tccactcatc aggcgcaagc caggttggat
      541 gtgccgggca atccttatag ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc
      601 cgcttggcgt acccagcgca tttttcaccg gatcggcgcg gaaatcacca taacgaacgt
      661 actgttcgtt cttctcgctg cggcgcattt cgatcagttt attttgctgc cagaaggcat
      721 taaacatcgg catagtcacg cgttgcgcac gagcaccttt ctgagcggca tcatagaaaa
      781 atttcagcca cgccatttca tctttacctt cggtatagat ctcttttccg ccaggtttca
      841 gtaattcagc aagatcggca aatacgtcaa aatcgttacg cgcttcaaat tgcggagcaa
      901 cagcctgctt catcggcaca atatgctggt tgctgtaatc accggtcatc gtcaagtcat
      961 tgcgctcaaa cgatgtggtg atcggtaata cgatatctgc atgtttagca gctgcggtcc
     1021 agtagcattc agaaacgacg atcatctccg gtttctgcca tgctttgatc agacgattag
     1081 tatcctggtg atgggtaaag ttaccgccac ccgcccacca gatcattttg atattcggat
     1141 acgtttgttc tttaccgtta tgctgatatt tgccaccagg attttccagc gcatcgacga
     1201 tacgggcaac aggaatcgcc gtcattccgc catcatcagc ggcctcgctg gcctgtccgg
     1261 caatcgccgc agacatttcc ggcagcacgc cgccaacacg cgtcggattg ccaccgttgg
     1321 agtaatgata agagaaacca aaaccgccgc ccggtgtacc aatctgccct aacatcgccg
     1381 ccagcgtcac cagcatccag tgtttctgtt caccatattg ctggcgctga attccccagc
     1441 cagccatcag cattgtgcga ttcgccgcca tcagttcagc gagtttgact atctgcgctt
     1501 ccggtacgcc agtaatttcc gccgcccagg ccgcgctctt cggagtatta tcgctcttac
     1561 ctgtcagata ctcttcaaac tgcggatacc cggtggtgta tttttcaagg aacactttat
     1621 cgtgtttacc ttgtgtcatc agggtatgcg caatccctaa catcagtgcc acgtcggtgc
     1681 ccatattcgg cgcgatccag gtggcattat caccaaagaa ctcgatggtt tcggagcgga
     1741 taggatcaat ggcaatcact ggtttgccag attttttcag ctgatggaag tattccagcc
     1801 cttgctcatc ggtactgctc caggcaattt ttaaggtatt cagcgggttc atcccccaca
     1861 gcaccacagc ctggctgttt tccagaatca gcggccagga ggtctgctgt tcatacacct
     1921 ctacagaacc gaccacatgc ggcatgatca cctgtgctgc cccggttgaa taatcgccgc
     1981 tatgcccgga ataaccgccc gccaggttca tataacgttg cagtaaggtt tgcgctttat
     2041 gcaacacgcc agaagagcgc cagccgtatg atccggcaaa aatagccgat ggtccgttag
     2101 ctttgcgaat gcgatcgtgt tgctcatgaa tcagttttaa tgcttgctcc cagctcacct
     2161 gtacataagt atcttcgcca cgacctttcg ccggttgcag tggattatcg agatagcttt
     2221 ttcttaccat cggatgctga atacgtgccg tggtgtgtac ctgatccgcc gccgtagact
     2281 gtaaggagtt cggtatggtt ttcgccagcg cgcctgtcga agaaacaatc ttgccgtcct
     2341 tcacttctac gctcatcgct ccccaacggc ccgcggtgag gattttaccg cccttctctt
     2401 ctgcccatgc gggtagcggt gctgccgatg tcaccaccag cgccccagcg gcaataccgc
     2461 tgtgtttaat aaattcacgt cttgttaatg tcataacttc ctccctgatc aacgaggatc
     2521 actgtttctc ggtaatatct ttggcgttgt actggaagta ccgcgtcaaa atgtccagtt
     2581 cgttttcgct catgctggtt cgcgcaccca ttcctttggc aatggacggc catgcattga
     2641 cggtgtaatg gtcagcggca ataggcgcat ggcaaccagc gcaataggta tcgtcaagtt
     2701 tttcagcgta ttgccatagc ggtttacggt ccgctaatgc gggatcggta agcgcaccct
     2761 gtaaagacgc ctgacgccat tgattgccgt attcgtcagc ctgccattcc ccgtttaccg
     2821 tgagcgcctt gataccttct tcacttaatg tggctagcgc cagccgttga cctgccgcca
     2881 ggtagagcgt gttttcactg ccctgcattt gataaccctg caacagaacg atcggctgtt
     2941 tgccactggc atcaacgacg gtgagatcgg caccaggatt cacggtagcc agctcgccta
     3001 tgcgagaagt tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac
     3061 tttccagctc atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc
     3121 ctttatgaca atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg
     3181 cagattcact ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg
     3241 cagagtcagt tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt
     3301 cgaacttatc gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg
     3361 cttttaattt agcaaataaa taatccatcc ctgactttgg aatatggcaa tcggcacatt
     3421 ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt
     3481 tactcatcga gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt
     3541 gcgccagcaa taacccgcag ccaccaacca caaccgctat cagcaaaaac aataacccaa
     3601 tgcgtttttt ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgta
     3661 taattaaata cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa
     3721 tcgataaaga atatgattta ttacaatgta atcattaatt gctaaggaat aacccagttg
     3781 ctatttagaa tatatattta tcagttttag caatttaaat cccataatca atgtgaatat
     3841 aaaaaatatt attagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat
     3901 cgttcgtttt ttatactgct cagggataaa aaaaggcccc tgttgaaatt gcaggggcct
     3961 ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat
     4021 ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgata taacgcgaat
     4081 actcgtccgc gtgttcatct gatgacatgg acaatccttg attacgataa cgcatcggtg
     4141 aggcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt
     4201 gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa
     4261 taagttccat aatttttgat aaaccttgca gtgcgtctga tttttgccct gatgtcagta
     4321 cccgggcaat gcccaattcc gcaagattat tgagtgtata taaagggtta gcgcacatat
     4381 cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc
     4441 gtggcatatc gacattcccg tcaacatcga gaacgcccgt caccagtcca ggaaaaccta
     4501 attcgcggac cgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa
     4561 aatcaccacc gcgtgggcga attatcggat gcacagggat cgtcacccgc tggcgcacgg
     4621 atttcagtac ccccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa
     4681 ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt
     4741 ccagtaatgc catttttgct ccttaattac gctgactgct cgctggcaac gatctcttca
     4801 atggaccacg gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc
     4861 ggtaatcgct catgctcacc ttttggtgaa ctggttttaa ctgagatccc cggtaggctg
     4921 agtccttcat cagaaagcaa agccagcgca cgggcattca gcgtttctgg atcacccggc
     4981 agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt
     5041 agctccacaa tagaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga
     5101 caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg ttcaaacccg
     5161 cgacgccagc gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc
     5221 agcggagtga tattcaaccc cagacggcgg gaaagttcat ctaatgcgtg ggtaaatcgc
     5281 ggtaaatccg atgcaatatc ctgcagctca tcgatagatt gccagttcgc cataatcact
     5341 cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgtgtcaacc aaccgctgat
     5401 ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg
     5461 aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt
     5521 gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt
     5581 ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc
     5641 agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact
     5701 gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat
     5761 cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt
     5821 cctggctgaa catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga
     5881 aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg
     5941 tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca
     6001 caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc
     6061 atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg
     6121 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg
     6181 taactacgta gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact
     6241 ggtcgacatc accatgacgc agaaccagat cacctatgat cgtctcaacg tgacgctgac
     6301 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     6361 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     6421 atttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta
     6481 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga
     6541 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat
     6601 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     6661 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     6721 cgatctgctg gatgaagccc tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     6781 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata
     6841 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     6901 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     6961 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     7021 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     7081 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     7141 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     7201 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     7261 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     7321 tgatcaggct tttttattgc catctaaatg tattctgaaa gtggacatgc cattgttttc
     7381 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     7441 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     7501 caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat
     7561 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     7621 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     7681 atagaacaag tgccacgcac aggtgtaaaa aaacctgata ccgaacaact taacttgctt
     7741 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     7801 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     7861 atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt
     7921 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     7981 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     8041 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     8101 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     8161 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     8221 caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga
     8281 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     8341 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     8401 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     8461 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     8521 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     8581 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct
     8641 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     8701 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
     8761 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt
     8821 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
     8881 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
     8941 tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
     9001 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
     9061 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
     9121 gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
     9181 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccagccag cgccccggat
     9241 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
     9301 gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt
     9361 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
     9421 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
     9481 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt
     9541 tttgttcgcg tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa
     9601 ataccaggtt cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca
     9661 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     9721 tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgct ggaatttgtg
     9781 ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca
     9841 tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga
     9901 tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat
     9961 cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag
    10021 cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg
    10081 cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga
    10141 gaatgacaaa caccacgata ccgatagcga aattgccacc aacgaggaag tgaccgaatg
    10201 cttcgaccac cttcccagcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg
    10261 ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg
    10321 cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg
    10381 caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg
    10441 acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt
    10501 ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa
    10561 tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga
    10621 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
    10681 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
    10741 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
    10801 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
    10861 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
    10921 cgccgtgcag cagctcgctg catctgacgg atccgccctt taacatgagg gtcaccttcg
    10981 ctttgtttga actcatcacg aatatcctgc cgcgacatac gcagcttttt caggtggctg
    11041 aagatttgta aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
    11101 gcgcatagcc caaccagatc catcgcatta cccatggcgg tagtcggaga ctcggccatt
    11161 aagcgcatca tctgcggcca gtgatgccag agataaaacc ccgtcacgct gccaaccagg
    11221 atggttttca aaattgcctt aagcaactcc gcgccagtct gtgccgagaa catccgttta
    11281 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
    11341 agccctccca gcatgaccgg agaaatgagc gccaccagta ccacgccgct aatcagcggc
    11401 agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg
    11461 tcattgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
    11521 gccagcgaca caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag
    11581 gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt
    11641 gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta
    11701 ttaagcgtga atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag
    11761 gcgggattta gggcttttgc tgccacacat caagcatagt gtgcgtttgt cggatgcggc
    11821 gtcatcgcct tatcagaccg cctgatatga cgtggtcacg ccacatcagg caatacaaat
    11881 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacgccggct
    11941 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
    12001 tcctgttccg ggatgttttc caacagcacc atcagcaact ggcgttcgat ctcctgaatg
    12061 acatccatca tccgcttaat gacctgcccg gtgagatcct gaaaatcctg cgccatcatg
    12121 atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt
    12181 cgtgtatctg ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
    12241 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
    12301 gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac
    12361 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
    12421 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
    12481 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
    12541 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
    12601 caccacatag ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc
    12661 tgcagtcacc attaacactg gcaatgccga catcgcgcca tccgcacgaa ttgttttcag
    12721 caattccagg ccatccatat tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
    12781 tgcctgcaac ttattgagag cgtcgacgcc atcttccgct tcctcaacat tattgaatcc
    12841 cagctcttta agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
    12901 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
    12961 gcactaattt ttgccaacat ttgctggctt acctggctaa gatcgaccac ttcgcagaca
    13021 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc
    13081 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc
    13141 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
    13201 aacacatcta ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg
    13261 taatttgcgc cactacgcga cagctccata tgccgatcgc ccggcgcaat ataggcatgc
    13321 cccggcaaga cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta
    13381 agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat gttgggtaat taacaatgcc
    13441 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
    13501 gcaccaatcg caatcagttt ttcagaactc aacaacggac ccgccttcag cgttgtcggt
    13561 actgacaatg gcttatgtgc tgcaaggctc gcctttgctg ccgtacgcac cttttcagca
    13621 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
    13681 tctattgccc ccagctccag cgcgcgcagc gtgacttctg atcctttgcc ggtcaaggaa
    13741 gaaaccatca caacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg
    13801 tccatccgcg gcatttcaac atccagcgtc agcacatcgg gattgaattt cttaatcaag
    13861 tcacgcgcga ccagcggatc aggcgcggtc gccaccattt ccatgtcgct atggctgttg
    13921 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
    13981 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
    14041 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
    14101 gcggaacaaa gcggcgcaaa atctcctgct gggtattttg atcgaagtag atcatgacgt
    14161 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtagattca
    14221 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
    14281 gccccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
    14341 catggcgata gataccgctt ctggcttttt ccagcacttc ggtgtcgata tcactggcaa
    14401 acactttcca gcgtccgggc gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
    14461 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
    14521 gtgcgtgatc cgcgagcaga gggaaatgat gtgcctcacg gaaaaatgcc gtcagattcg
    14581 tggtcagcga attgataaac gcctgccact caccgctgtg ctgattagat tccagcaagt
    14641 tcagataatg accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt
    14701 aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac
    14761 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    14821 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    14881 ctggcgcaat ttgtaactgc accgactcat gtcgtgctac ttcatgttct tcaagggtaa
    14941 ataccgccac acgcgacgaa agatggtcgg cctgattcgc cagttgttcc gttgccaccg
    15001 ccgcttcttc taccagcgag gcgttctgct gagtcacctg atccatctgg ctgacagcct
    15061 gtgcaacctg ctcaatcccc cgctgttgtt cttccgacgc agaggcaatt tctcccataa
    15121 tgtcgttcac gcgggtcacc gaactgacaa tatcgatcat ggtcgcggcg gcgttattca
    15181 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    15241 ttgctgcctg cgcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    15301 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    15361 tctggaaagc aattccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtgg
    15421 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    15481 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    15541 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    15601 tacgacttga gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct
    15661 ggcttccctt acgcacatca cttaccgtcc cacgcaaagc ctgctgcatg gtcttcagac
    15721 tggcaaaaat ggcggtgatc tcattacgac catataccgc aatcggacgc gccagattac
    15781 ccgcagcaat gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc
    15841 gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca
    15901 ccagtgccga aatctgatag ttacgctggc tttgcgcact ggcggcttcc agcacatggt
    15961 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg
    16021 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    16081 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caatcccctg acgtgctcgt
    16141 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    16201 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagtgcc gtcagagtgc
    16261 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    16321 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    16381 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    16441 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    16501 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    16561 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    16621 gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt
    16681 ccagcgcagc ggcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    16741 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    16801 ccgatgcaat ctcgcccata atgtcagtca cgcgagtgac agcattgacg atattgttca
    16861 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgtgag acggagtctt
    16921 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    16981 cacccgccac cacggcaaaa ccacggccct gttcacccgc acgcgcggct tcaaccgcgg
    17041 cattcagcgc gaggatatta gtctggaagg caataccgtc gataacgctg ataatgtcgg
    17101 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    17161 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    17221 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    17281 gcgcggatgc ctgctgttca gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    17341 gggtaccggc atagatggca tctgaacctt cgcggacatg agtgacggtg tcagtcaaag
    17401 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    17461 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    17521 gcggagtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    17581 ccagcgcgat aaccgccagt tgccactggg caaattggta atcatctgcg ttgtcagtga
    17641 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    17701 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatctagat
    17761 aatcaatcag ttcagttaac gctgtgtaat agtttttata tttttcatca atattacgac
    17821 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgcgtcgctg
    17881 cctgcgccaa tgttttcctg gcgctatcga gcaattcaac tttggcgtta ctttgttgat
    17941 tggaggaatc catcatcatc cgtaccgctg aacgactcag gttaatgcgc gtttgcagca
    18001 ttaaatccca ggttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa
    18061 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    18121 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    18181 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag gaggggggaa actttattgc
    18241 tgatgccacc cgccgcgaaa ttgaaataaa aaacccgatg cgcagatcat cgggttcatt
    18301 tcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct aacagcgcca
    18361 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatccgg tcgcccagtg
    18421 cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc aaattccggt gccggacgaa
    18481 tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc aaccacgatg ccgaccaccc
    18541 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    18601 tgaacttaat tcgtaagtca acaatcggca caataacgcc gcgcagattc gtgacgcctt
    18661 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    18721 ctttcaggat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    18781 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    18841 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    18901 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    18961 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgctgac caattaattg
    19021 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt
    19081 ggcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    19141 ttcgccccgc acttccagca cccgctcgcc gccggccagt ggatgcagat cggcttcacg
    19201 gggttgcagt gattccatga cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    19261 tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc
    19321 agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    19381 gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctctgccg tggagaagcc
    19441 tggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cagtcaaacc
    19501 ttgcgaggcc gcttttgcca gaattcgctc acggtttagc cccgccccat cgtcggtcac
    19561 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    19621 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    19681 caggtgggtc agcgggtcga taatgcgttc tatcaggctc ttgtcgagct cagtggaact
    19741 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gccagatcac gcaccagccg
    19801 gggatagcga ctaaaaacat attccatcgg catcatgcga atcgacatca ctgattcctg
    19861 caaatcacgg gcgttacgtt gtaactgccc catgctggtt atcaaatcac catgattaac
    19921 cgggtccagt tcgctggaac gctgggcaag catggactgg gtgataacca gctcgccgac
    19981 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    20041 gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg ccagtttaag
    20101 cactggtggg gtggatattt ttggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    20161 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    20221 tgccgagagc gaatccgccc ctttcaccac gtcagttaac gttgtcagat gtcccagttc
    20281 ttcttccagc aggtcgactt ccccggcctt caggcgcgaa aggataattc gtcgcggcga
    20341 ctgactgcga ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    20401 tgcggatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    20461 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    20521 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    20581 tctggcttca tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    20641 aaaagttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    20701 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatatcag ccaacagttc
    20761 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    20821 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    20881 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    20941 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    21001 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcta tccaaccctc
    21061 cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc agaaagctcc cagttgctgt
    21121 aacctttctc accactggcg taggggaaat catcggtatg acccgaaagg ctaatacggt
    21181 tgggaatacc attcagcaca ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga
    21241 catcggcact gccggttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    21301 gaccttcctg gaccagatcg attttgagat ggggacgtaa cgcccgcagt ttcggatcgg
    21361 actctatcaa ctggtcgaga tcaccccgca atttccacaa tcgactttgc tccatgcgtt
    21421 ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    21481 caccaccgcc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    21541 tcgccagtgg agtccggaag tactccgcaa tctgaatcaa ctcttttggg ctggagatgg
    21601 agatcagcca catcaccaga aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa
    21661 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    21721 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    21781 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    21841 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagaaa gcagagtgac
    21901 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctag
    21961 tggggaaata aatccgtaag ccaataaaat accgaggaaa gtccccacca tcgcatgtgc
    22021 gataagcgcc cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    22081 cattacagcc gcgacaatac caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt
    22141 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gggcttcgat
    22201 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaaatcaag
    22261 catgacgcta tccgcgagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    22321 ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    22381 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    22441 cggcagcgct ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    22501 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    22561 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    22621 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    22681 cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgttgcgcc
    22741 acaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    22801 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggtt gacataagct
    22861 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    22921 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
    22981 taatgccagc agtggtcctt cttctgcttg tgggcactgt tcaaggtata aacggtaggc
    23041 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcatt
    23101 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    23161 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta taaagtttta tcaggcgtcc
    23221 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    23281 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    23341 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    23401 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    23461 atcgtctggt ggctgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    23521 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    23581 cgaaacatag cggacgcttt gtcctgaaca atcaaacgct gtgcaagtag taaatatgac
    23641 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    23701 aaccaacttt atttttatgc ggtttcaccg caccccgtga tgtcgccggg aagacccggc
    23761 aaaaaataat tagcattaga atagttgcga taagctgcaa taagcagagc cacctttttg
    23821 gtttaataag tccttacaaa tagaaggggg tctttacact tatctaagat ttttcctaaa
    23881 tcgacgcaac tgtactcgtc actacacgca catactacgg aggagggctg cgattttcaa
    23941 taatgtgtga tgcagatcac acaaaacact caattactta acataaatgt ataagccata
    24001 cttttgtttt gggtgtattt caatctgtta aaaagttttt cgctacgcta gcaagcaaaa
    24061 atgaaacagg aataatcgaa atgggatgtt gcgcacagtt aaaataactc accgtaaata
    24121 atcatctgct ataaataatc actttcatgc aataccagat aagctatttt taaacagaca
    24181 cttaccgcac aacaaactaa ttagatacaa agaaaggcaa caatcaactt aatgttaata
    24241 aaatgttttg catatattaa ccacttgttt aatacgggga atcagtacat tattttctta
    24301 taattacagt tgaaatcata tgatcaccgg gagtgattga ttaattgtct ttactaataa
    24361 tcagactaat atttacctgt ttgactgagt tgggattgcg tcgtttctcc attaggagta
    24421 aagctttaat gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat
    24481 tcttgtcgct gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat
    24541 cgccaggcca gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta
    24601 caatcaatta gccgcgccga tgcttgaaga tttacgcagc gtgatgcagg aagaaacgca
    24661 aagctttctt gataagttaa ttcaggatgc ggggtatccc gttgacaaga cgtttattgc
    24721 ctacggtgag ttaagcgaac atattctgga agtatgtcac aaacatcatt tcgatttggt
    24781 gatttgcggt aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt
    24841 tattgcctca agtgaggtcg atgtgctgtt agttccactt acgggagatt aaccgctcct
    24901 atgcaagctt tggaaaggta gcaactttat cgcgctgctg gctttccgcg cttcgcggaa
    24961 ctatctgctt taggtcgcta atgaagcact cctgccagtg gttaatatcg tttttcacga
    25021 taacgtccag catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac
    25081 gatccagcgc agctgcaact tcgtcacgat cgtaggggtt aacaattaac gccgacgtta
    25141 actcgtttgc cgctcccgca aattgcgaaa gaacaagaac gcccgggttg gctgggtcct
    25201 gagcagcaac atactctttt gctaccaggt tcatcccgtc acgcagtggc gtcactaagc
    25261 ccacgtcaga gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat
    25321 aataaagcgg cgtccagcct aattgcccgt atttaccatt aattcgtcca gcttcatttt
    25381 cgagctgatg acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct
    25441 gggtataacg aattttacca tgatgctgcg gatatttttc cagcaacgct tcataggcga
    25501 gaaaacgctc tggcaaacct ttggaataat ccagccgttc gacagaaaag atattttgta
    25561 cgtttttcag ttccgcttta agttgcgcca gttttggcgg cagtggcccg gcagcctgtt
    25621 tggctatttc tttcggttca atgccgatcg ggtagacttc tgttcgaaat gctttgcccc
    25681 aggctgtatg gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa aggcaatcta
    25741 ggaacgccag acgatcgttt tctgtctgga aacccagcaa atcataatca caaagctgtt
    25801 caagcaaggt gtcatatgtc ggcagcgcgt tgaagatttc cggtgtcggg aaaggaatat
    25861 gcagaaagaa accaatgcga ttattcactc cccgtttgcg taattcatgc gcaaagggca
    25921 acaggtgata atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat
    25981 ctgccagcaa cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca
    26041 gatcgagccg ataatgaaaa gcgggccaga gaacggcatt ggagaattgg ttgtagtatt
    26101 cgtcaaggtc ctgttcgctg aggttaaaag aggcccacgt aatgttacct tttttcacct
    26161 tttttagcgg ctgatcctca ttccctgttt caccactcca gccaaaccac agtccgcctg
    26221 cggctttcag tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcgt
    26281 ctggtggtgc aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta
    26341 ttttctcttt tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggc
    26401 acacccgcca gtcgccatga tgcctgagtt gcacctgtgc caatttttac tgacattccg
    26461 cccagtcggt taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaatacg
    26521 ggcgttcgcc cgataaaggg agcttcctgc ataaaagctg caattgcctc acctttactg
    26581 gtacctctcg gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag
    26641 atctgagtaa tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga
    26701 taatgcagcg caaaagccat ccctttcgcc tccagctccg cgccgggata ctgagcgatg
    26761 actgtatgca gttgcacgct aatatcacgc gcaatcgcat ccggcagatg aacgatatgt
    26821 gttttaccat tgatgtcacg gcgctctgcc ccatgcacgc ccgctaacgg gaagcgataa
    26881 ggttttgcca gtgcgtcaag ctccaccatt gagcgccctg atatcaatgc caatgcacca
    26941 tcacttgcgg ttgccagtag ctgaagtcct tgcagaatat tgtcaggcac aacgacctga
    27001 tcgggatgcg gtttgatttc cgccagcgtt ccatcgagat caaaaaacca ggcatatttc
    27061 gcggatagtt caggggcttc ggttaacggt tctgtcaccc ggttctcctc cttctctttt
    27121 cttattgttt tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg
    27181 tcgccatttg caacgaagaa cagccaaccg ttatggcggt tggctgtggt gggaaaaaac
    27241 gctaaattgt tgcagaaaaa agcatcagac agtgcgtttc gctttttgct tgtaacggtc
    27301 gaagatcacc gctgccagca ggattaagcc gcgaaccacg tactgcgcga aaggagaaat
    27361 attaagcagg ttcatggcgt tttccacggt gcctaaaatt aagatacccg ccaccacata
    27421 tgagattttt ccgatgcctc ctttcagaga aacgccacct aaaacgcagg cggagataac
    27481 aatcagctcg taaccaatcg acgtcattgg ctgcccactg gtcatacgtg aagccagaat
    27541 aattccggct atcgcagata ccaggccaga gagaacaaag ataataattt tggtgcgaac
    27601 aaccggtaca cccgccagac gcgcagcctc ttcgttcccg ccaatcgcca gggtgttacg
    27661 accaaaggtg gttttattca gcaacaaacc aaagataatc agacacgcga cggtgagcca
    27721 gattggcgca ggcagaccga accagttagc gtaaccaagg gcaaagaagc tttcatcttc
    27781 gataccgacc gctttaccgt ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt
    27841 tgccagtgtt gtgatcagag catttatttt cagtttggcg ataacaaagc cattgaccag
    27901 gccacagaga atgccaagca gcaaccccgc tgccacgcca atccacaggc tttcagtcag
    27961 gttgataacc accgccgtgg tgacacccgc acaggcaatt acggaggcga cagaaaggtc
    28021 aaagtcaccg gaagcaaggc agaacaacat gccacacgcc accatccccg acatggaaat
    28081 tgccaggccc aaccctttca tattaatgaa ggtggcaaaa tttgggacaa aaatggcaca
    28141 ggcgataaag agcaccgcaa acaccaccag catgccgtac tgatcccaga tacgcccgaa
    28201 gctgaatgac gacttaggtg cgccagaccc cgatgtagaa acagaagaca tcatactctc
    28261 cttactcagg caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca
    28321 tctgcctgct cgtgtaacaa ttcaccggcg atttcacctt cccgcatcac cacaatccgg
    28381 tcggcaacgc cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc
    28441 gccgccagcg cataaatcac gttgtaaatt tcatgcttag caccaacatc aatgccgcgc
    28501 gtaggttcat ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct
    28561 ttttgctgat ttccgcctga gagattcatg atcagttgct cagcgcccgg cgttttgatg
    28621 ttgagcgaac gaatgtggtg atcggcattg ttttcttccc aaccgttgtt gattacacaa
    28681 ccgccgagca catgtttacg tctggcactg atgttgatat tgtcgcgaac ggagtgcacg
    28741 ggaataatgc cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg
    28801 tggctcggtt tacgaatatc gatcggctgt tggtcgatat aaacctgacc ggcggtgatt
    28861 tgcgtcccgc caaacaagcc tttcattaat tcgctacgcc ccgcccctac cagaccaaac
    28921 agcccaacaa tttcaccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct
    28981 ttcacagcat caagacgcag gcgctcctcg ccataactac gaggttgcca gccgtagata
    29041 tcgccaatgt cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac ctgctgcata
    29101 tcggtaaagg ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca
    29161 aatatttctt ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca
    29221 cgaataacgc ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt tggctcatca
    29281 aaggcgataa ttttagcgtt acgcgccagc gccttggcga tttcaaccat ctgccactga
    29341 ccaatggaga gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt
    29401 tgtaaacccg cctcataatt cagcaatgag cgattcacaa tgccgccttt atgcggcagc
    29461 tggccgagat agatgttttc cgcgacggtc atttccggca cgagatgcag ttcctggtaa
    29521 ataatcgcca cacccgcgtt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca
    29581 ttaatcacta cagaacccgt ggttggcgca tagttgccgc tgaggatttt taagagagtt
    29641 gattttcctg cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa
    29701 ctaatatccg tcagcgcctt aacgccggga aacgttttac cgatgccgcg aaatgagaga
    29761 tacggggtag actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc
    29821 atcatgcaga ggggaatttt tccggcaaat tacttaccgc ctaaaccttt tttctccagt
    29881 tcttctttaa agttgtcacg cgtgatcagc accacgtcgg taacttcggt aaattttggc
    29941 ggttcaacgt cttttgctac ccagttgtaa agcatttcgc tggatttata gccatgtacg
    30001 tccgggcttg gcagcaggga accgtagaag ccggttgcct gtgctttaga cagttcgctc
    30061 accgcatcca caccgttaat gccaatgccg atgatatcgg cagctttaaa gccctgacct
    30121 tccgtcgcgc gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt
    30181 ttaacttccg gatgttgaac cagcattgag ttggcagcgt caaatgcccc cgggatgtcg
    30241 ttagatttgg taggtacctg ataaatttgt ttttccggga atccggccgc tttcagcgca
    30301 tccatagaac ccgttgtacg gcgacgtgcc gtatccagtt cgttggcggt aatcgccatc
    30361 accgcgcttt ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctgaccc
    30421 tgacgttcgc caattttagt cgccgccatc atcaccagcg gaacggtatc cattggctta
    30481 cctttggcgt taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct
    30541 ttcgcgacga tggcagagcc gagtttgggg tccggagtac aaataacgaa accttttgcg
    30601 ccactggcag ccaggctgtc gatcgcgttc aatgtttttt cgccatccgg cacggcaatc
    30661 ttaataacct caaaccctaa atccttcccg gctttatcgg caaacttcca ttcggtctgg
    30721 aaccacggct cttccggttg cttcaccaga aaaccgagct tcaggttctc cgccatagcg
    30781 gattgtgaca taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg
    30841 gttctctcca gctttagtgt cgttttgtgt agggcaaaaa cgaatgactt tcgttaaatt
    30901 aatcggaaaa caaagcatta ccttttatct aaaagataag tgaccgtgtt gacatagttt
    30961 tagcgagaaa ttaattctcc ataggagagc aatatcacat cgcagaatta cagtgagaac
    31021 gtgcataaat ttagcgggaa aagacataag ggaaagccaa tttgtcagac aaattgtcga
    31081 atgcacagca gattaatcca taagattagc ctggaaatcc ttgttgtctt tggtacccat
    31141 gcgggatgtc ttctttttaa ccagtcaata ggccgcatta cctggcgttg agtttttgaa
    31201 atggtgtaat aaccgcaact caaagatgtg gaaaatgcac gtcattcatt tcgccattaa
    31261 ttatcactgt gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga
    31321 aaaaacgttt tataaaatca tcacttcatc atgaattcaa attcattgat taatatcaac
    31381 aagatacaaa aagcactatc attaaaattc attgcagtta cattgatttc atcaatgaaa
    31441 tgtaaaaata tataaacttg atgatttaag cattttctta tacccgttca gacgttattc
    31501 ttatttctga tcatcgtcag aattgactcc acgatcacat ttcggaccga cagaaaggaa
    31561 ttattctgca aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat
    31621 ataaggatat taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac
    31681 cgcgagtttt acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt
    31741 ctgaacggca ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg
    31801 cgcatgttta actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt
    31861 cccggtgaaa aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag
    31921 caacgttcta gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca
    31981 accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa
    32041 accattcttg atgaagtgcg cagcgcgaaa cttgcgggta tgtgccctgt gcagaccgac
    32101 caacatgttc tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat
    32161 tgcgccgatg aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta
    32221 atgggattca ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc
    32281 ccgcatggtg accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg
    32341 actggacatt ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg
    32401 tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg
    32461 ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc
    32521 ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt
    32581 ttgttatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa
    32641 catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt
    32701 gttattgtct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac
    32761 aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat
    32821 ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc
    32881 gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact
    32941 tctgattctg actctttcat taataacgct ggcggggtgt acggttactc gccaagccca
    33001 tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt
    33061 gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca
    33121 ggaaggctat acccacgcga tcccctttgg tcagccagta ggcaactgta gcctttttgc
    33181 gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt
    33241 ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta aataaatagc
    33301 ggtaatgcgt tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg
    33361 caaataataa aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc
    33421 actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg
    33481 tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt
    33541 gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt
    33601 gattacctga ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct
    33661 gaatattcct cacttgatga attattccag gaaacctata aacacgaaca attaatcacc
    33721 cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat
    33781 ttcctgcaat ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt
    33841 gataaattaa gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc
    33901 tctaccctcg acacacaaaa ctaatgctct ccggcggcag gtttactgcc gccgtttcaa
    33961 atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc gtctttcgaa ggtataaatt
    34021 tgccttttgc tgccagaaac gctaccagct ctcccgctgt catcccttct gccgagcagg
    34081 tgtgaaaacg tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg
    34141 tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca
    34201 tcgtccctcc tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag
    34261 cgcaggactg aatgcttttt attgtacatt tatatttaca ccatatgtaa cgtcggtttg
    34321 acgaagcagc cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga
    34381 cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa
    34441 ttggcgcagg catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt
    34501 taatcttgtt gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg
    34561 tgtaccagca tgttccggca gataccggtc tgggcacgct ggcaaaacgc tatctgggac
    34621 gctacggtca atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg
    34681 catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt
    34741 caatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg
    34801 tcgggacatc gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc
    34861 tggtggtgat gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc
    34921 cgttgcaaca ggggctggct ctgtcagcaa tcccggtgat ttttacctca tttggttttc
    34981 acggtagcgt gccgagtatt gtcagctata tggatggcaa cgttcgtaag ctacgctggg
    35041 tgtttataac cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcaacgc
    35101 ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgct ggattaaacg
    35161 ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt
    35221 tatttgctga tttagccctc gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt
    35281 atctggctga tttatttcag cgttcaaata ccgttggtgg acg
//
LOCUS       DAFATA010000042        33271 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFATA010000042 DAFATA010000000
VERSION     DAFATA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.043"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..288
                     /gene="dnaT"
                     /locus_tag="LGJ20_002420"
     CDS             <1..288
                     /gene="dnaT"
                     /locus_tag="LGJ20_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBK2386471.1"
                     /translation="PMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELASFIAYW
                     QAEGKVFHHVQWQQKLARSLQIGRASNGGLPKRDVNTVSEPDSQIPPGFRG"
     gene            291..1028
                     /gene="dnaC"
                     /locus_tag="LGJ20_002421"
     CDS             291..1028
                     /gene="dnaC"
                     /locus_tag="LGJ20_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBK2386472.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            1077..1571
                     /locus_tag="LGJ20_002422"
     CDS             1077..1571
                     /locus_tag="LGJ20_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBK2386473.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            1825..4116
                     /gene="opgB"
                     /locus_tag="LGJ20_002423"
     CDS             1825..4116
                     /gene="opgB"
                     /locus_tag="LGJ20_002423"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBK2386474.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRDDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(4255..5277)
                     /gene="lgoD"
                     /locus_tag="LGJ20_002424"
     CDS             complement(4255..5277)
                     /gene="lgoD"
                     /locus_tag="LGJ20_002424"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBK2386475.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            5416..6330
                     /locus_tag="LGJ20_002425"
     CDS             5416..6330
                     /locus_tag="LGJ20_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2386476.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVLVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            6545..7906
                     /locus_tag="LGJ20_002426"
     CDS             6545..7906
                     /locus_tag="LGJ20_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2386477.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(7955..9619)
                     /gene="tsr"
                     /locus_tag="LGJ20_002427"
     CDS             complement(7955..9619)
                     /gene="tsr"
                     /locus_tag="LGJ20_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBK2386478.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(9738..10184)
                     /gene="hpaR"
                     /locus_tag="LGJ20_002428"
     CDS             complement(9738..10184)
                     /gene="hpaR"
                     /locus_tag="LGJ20_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBK2386479.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            10456..11745
                     /locus_tag="LGJ20_002429"
     CDS             10456..11745
                     /locus_tag="LGJ20_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBK2386480.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            11742..13208
                     /gene="hpaE"
                     /locus_tag="LGJ20_002430"
     CDS             11742..13208
                     /gene="hpaE"
                     /locus_tag="LGJ20_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBK2386481.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            13210..14061
                     /gene="hpaD"
                     /locus_tag="LGJ20_002431"
     CDS             13210..14061
                     /gene="hpaD"
                     /locus_tag="LGJ20_002431"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBK2386482.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            14071..14451
                     /locus_tag="LGJ20_002432"
     CDS             14071..14451
                     /locus_tag="LGJ20_002432"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBK2386483.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            14562..15365
                     /gene="hpaH"
                     /locus_tag="LGJ20_002433"
     CDS             14562..15365
                     /gene="hpaH"
                     /locus_tag="LGJ20_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBK2386484.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            15376..16164
                     /gene="hpaI"
                     /locus_tag="LGJ20_002434"
     CDS             15376..16164
                     /gene="hpaI"
                     /locus_tag="LGJ20_002434"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBK2386485.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            16339..17715
                     /gene="hpaX"
                     /locus_tag="LGJ20_002435"
     CDS             16339..17715
                     /gene="hpaX"
                     /locus_tag="LGJ20_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBK2386486.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            17725..18615
                     /gene="hpaA"
                     /locus_tag="LGJ20_002436"
     CDS             17725..18615
                     /gene="hpaA"
                     /locus_tag="LGJ20_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBK2386487.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGWEHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            18866..20428
                     /gene="hpaB"
                     /locus_tag="LGJ20_002437"
     CDS             18866..20428
                     /gene="hpaB"
                     /locus_tag="LGJ20_002437"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBK2386488.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            20446..20958
                     /locus_tag="LGJ20_002438"
     CDS             20446..20958
                     /locus_tag="LGJ20_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBK2386489.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            21351..23501
                     /gene="btsT"
                     /locus_tag="LGJ20_002439"
     CDS             21351..23501
                     /gene="btsT"
                     /locus_tag="LGJ20_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBK2386490.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            23551..23754
                     /locus_tag="LGJ20_002440"
     CDS             23551..23754
                     /locus_tag="LGJ20_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBK2386491.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            23765..24721
                     /gene="yjiA"
                     /locus_tag="LGJ20_002441"
     CDS             23765..24721
                     /gene="yjiA"
                     /locus_tag="LGJ20_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBK2386492.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELNYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(24799..27339)
                     /locus_tag="LGJ20_002442"
     CDS             complement(24799..27339)
                     /locus_tag="LGJ20_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HBK2386493.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(27691..27855)
                     /locus_tag="LGJ20_002443"
     CDS             complement(27691..27855)
                     /locus_tag="LGJ20_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HBK2386494.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            28032..29444
                     /locus_tag="LGJ20_002444"
     CDS             28032..29444
                     /locus_tag="LGJ20_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK2386495.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQSYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(29686..30630)
                     /locus_tag="LGJ20_002445"
     CDS             complement(29686..30630)
                     /locus_tag="LGJ20_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBK2386496.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            31097..32329
                     /gene="mdtM"
                     /locus_tag="LGJ20_002446"
     CDS             31097..32329
                     /gene="mdtM"
                     /locus_tag="LGJ20_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HBK2386497.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEW"
     gene            32370..>33271
                     /locus_tag="LGJ20_002447"
     CDS             32370..>33271
                     /locus_tag="LGJ20_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBK2386498.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPVLLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLS"
BASE COUNT         7802 a         8888 c         8543 g         8038 t
ORIGIN      
        1 cccatgggga aattcgccat gtatccggac tggcaacccg atgccgactt tatccgcctg
       61 gcggcgctat ggggcgtggc gctaagagag ccggtgacca ccgaagaact ggcctcattc
      121 attgcctact ggcaggcgga aggtaaagtc tttcaccatg tgcagtggca acaaaaactg
      181 gcgcgcagcc tgcaaatcgg tcgtgccagc aacggcggac tgccgaaacg agatgtgaat
      241 acggtcagcg aacctgacag ccaaattcca ccaggattca gagggtaacg atgaaaaacg
      301 ttggcgacct gatgcaacgc ctgcaaaaaa tgatgcctgc ccatatcaaa cctgcattca
      361 aaacgggtga agagctactg gcctggcaga aagaacaagg agcgatccgc tccgccgctc
      421 tcgaacgtga aaatcgggcg atgaaaatgc agcgcacctt taaccgctcc ggtattcggc
      481 cactgcatca gaactgctcc tttgagaact atcgcgttga gtgtgaaggg cagatgaatg
      541 cgttaagcaa agcgcgccag tacgtcgaag agtttgacgg caacatcgcc agcttcatct
      601 tttctggtaa gccaggaacc ggcaaaaacc atctggcggc ggcaatctgc aacgagctgc
      661 tgctacgcgg taaatccgta ttgatcatca ccgtggccga tattatgtcg gcgatgaaag
      721 ataccttcag gaatagcggt accagtgaag aacaactgct taacgatctg agcaacgttg
      781 atctgctggt gatcgatgag atcggcgtgc agaccgaatc gaaatacgaa aaagtgatca
      841 tcaaccagat cgtcgatcgc cgctcttctt ccaaacgccc aaccgggatg ctgaccaaca
      901 gtaatatgga agagatgacc aagttgctgg gcgaacgcgt gatggaccgt atgcgcctgg
      961 gtaacagttt gtgggtgatc ttcaactggg atagctaccg tagccgggta acaggtaaag
     1021 agtattaatc cccgtttctg gcggtatact gcgacacaat tccaccttga gtaatgatga
     1081 tgaaaactgt taaacatctt ctgtgctgtg ccattgccgc cagcgcatta atttccaccg
     1141 gggtgcatgc tgcgtcctgg aaagatgcgc tctccagcgc cgccagcgaa cttggcaacc
     1201 aaaacagcac gacacaggaa ggcggttggt cgctcgcgtc attaactaac ttgcttagca
     1261 gcgggaacca ggccttaagc gcagataaca tgaacaacgc cgcaggcatt ctgcaatact
     1321 gcgcgaagca aaagctggcg tcggtaaccg atgccgaaaa catcaagaac caggtgctgg
     1381 aaaagctggg cctgaacagt gaagagcaaa aagaagacac caactatctg gatggcattc
     1441 agggtttgct gaaaaccaaa gatggtcagc aactcaatct ggataacatc ggaacgactc
     1501 cgctggcaga aaaggtgaaa accaaagcct gcgatctggt gttaaagcag gggctgaact
     1561 tcatttcctg aaccgcgaaa cgccgcgttt tagctgttac ccggctgacg cggctctctc
     1621 ctgccctttt ccctgctcct ggacggtttt ccccctgtta cacgggaagc gaccgcgatc
     1681 aaaaaagtct gtttataaac taattcctaa caacatcgca tttttttgac actagaattg
     1741 cagcaatatg gcgacgccat tacattgtgc ttctcaaaaa gcttaaacgt tggctaaacg
     1801 gcccggttta gtgaggatcg cccgttgtca gaactactct ctttcgccct ttttctcgcc
     1861 tctgtgctga tttacgcatg gaaagcggga cgtaacacct ggtggtttgc agccacatta
     1921 acggtgctgg ggctatttgt cgttttaaat atcaccctgt ttgccagcga ctattttact
     1981 ggcgatggta ttaacgacgc ggttctctat accttaacca acagcctgac cggtgctggc
     2041 gtcagcaaat acatcctgcc gggtatcggc attgtgctgg ggctgacagc ggtgttcggt
     2101 gcgctgggct ggatcctgcg ccgtcgtcgc catcatccgc accattttgg ttacagcctg
     2161 ctggcgctct tactggcgct gggttcggtg gacgccagcc cagcatttcg tcagataacg
     2221 gaactggtga aatcccagtc acgcgatggc gacccggact ttgcggctta ttataaagag
     2281 ccgtcgaaaa ctatccctga cccgaaactc aacctggttt atatctacgg cgaaagtctc
     2341 gagcggacct attttgataa cgaagctttc ccggatctca cgcctgaact gggcgcgttg
     2401 aaaaatgaag gcctggattt cagccacacg cagcagctgc caggaacgga ttacacgatt
     2461 gcgggcatgg tggcttctca gtgcggcata ccgctgtttg ccccctttga aggcaacgcc
     2521 tccgcctctg tctccagctt cttcccgcag aacatctgtc tgggcgatat cctgaaaaac
     2581 tcgggttatc agaactattt cgtgcagggc gcgaatctgc gttttgccgg taaagatgtg
     2641 ttcctgaagt cgcatggctt cgaccactta tacggctcag aagagctgaa aagcgtggtg
     2701 gccgacccgc actatcgcaa cgactgggga ttctacgacg ataccgttct cgatgaagcg
     2761 tggaaaaagt ttgaagagct ttcccgctca ggtcagcgat tctcactgtt taccctgaca
     2821 gtcgataccc atcacccgga tggttttatc tcgcgtacct gtaaccgcaa aaaatatgat
     2881 tttgacggta agccgaacca gtcattcagc gcggtaagtt gcagccagga gaatatcgcg
     2941 gcgtttatca acaaaatcaa agcgtcaccg tggtttaaag ataccgtcat cgtcgtctct
     3001 tctgaccatt tagcgatgaa caacacggcg tggaaatacc ttaataagca ggatcgcaat
     3061 aacctgtttt ttgtcattcg tgacgacaag ccgcagcaag agacgctggc agtgaagcgt
     3121 aacacgatgg ataacggcgc gacggtgctg gacattctcg gtggcgataa ctatctcgga
     3181 cttggtcgta gcagtttatc cgggcagtcg atgtcggaaa tcttcctcaa tatcaaagag
     3241 aaaacattgg cgtggaagcc ggatatcatc cgcctgtgga aattccctaa agagatgaaa
     3301 gagttcacta tcgaccagca gaaaaacatg attgccttct cgggtagcca tttccgtttg
     3361 ccgctgctgt tgcgggtttc agacaagcgc gtagaaccgc tgccggaaag tgaatactca
     3421 gcaccgctgc gtttccagct ggccgatttc gctccacgcg acaatttcgt ctgggttgac
     3481 cgttgctaca agatggcaca actttgggct ccggaactgg cactctccac cgactggtgt
     3541 gtctcgcagg ggcagcttgg cggtcagcaa attgtccagc atgttgataa aacaatgtgg
     3601 aagggcaaaa cggcatttaa agatacggtc atcgacatgg cgcgttacaa aagcaatgtc
     3661 gatacgctga aaattgttga taacgatatt cgctacaaag ccgacagttt catcttcaac
     3721 gtcgccggtg cgccagaaga ggtgaaacag tttagcggga tttcccgtcc ggagtcgtgg
     3781 ggccgctggt ccaacgctca gctgggcgat gaagtaaaaa tcgagtacaa gcatccgctg
     3841 ccgaagaaat ttgacctggt gattaccgcc aaagcatacg gcaataacgc cagccgtcct
     3901 attccggtac gcgtaggcaa tgaagaacaa acccttgtgc tgggcaatga agtgaccacc
     3961 accacactgc atttcgataa cccaaccgat gccgacacac tggtaattgt gccgccggaa
     4021 cctgtctcaa ccaacgaagg gaatatcctc ggacactcgc cgcgtaagct cgggatcggc
     4081 atggtggaaa ttaaagtggt ggaacgtgaa ggataattcc tgactgatgt gtaggtcgca
     4141 taaggtgcga cctacacacc ttctgcggcc tacaaaacat cattgagaaa cagaaaaaaa
     4201 cagcgcccaa cacttccccg gacgctgcat ggcaacttta ataatctcgc cttatcagaa
     4261 agtaattaca cccttaatca actcacgatt gttaatcaca tcgcgctcgt aggtttctgc
     4321 cagcgtggcg aacggatagc gatgggttaa catcatgtcg gcagtgattt ttccttccgc
     4381 catcagtcga ccgactttag caaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt
     4441 cgtttctttt ttatggaatt ccggatcgga gaactgcaac tcacctttaa acaggccgac
     4501 aaataccacc gtgccgccgt gacgaatcaa attcacggtg ttattcatcg catgttgatt
     4561 acctgtcgcg tcgatcactt tctgcgccag cgaaccacca aactgcgccc gtagctgcgc
     4621 gtcaaaatct tcggctgacg ggtccagtaa aggtaattcc agacgcgttg ccacatgttc
     4681 acggcgcgcc ggactggtat ccgccaccac cacctgtgcg ccatcggctt tagcgattgc
     4741 cgccgcgccc agaccgattg gccccgcccc gaccaccagc acctgctcgc cgggagcaat
     4801 ggctgcgcga cgcaccgcat gagcgctaat agcgaaaggt tcaatcaatg ctgccgcctg
     4861 cgggtcaata ccgtctgcgg gcaaaatgtt cgccaccggc acgctcaaat actcactaaa
     4921 accgccatcc tgatgcacgc caatgactga aattttttca cagcaattgg tacgcccgct
     4981 tttacacgcc gggcattgct gacaggcaac ataagggatc acagcaactt gctgaccatt
     5041 tttaagatca gcaatatttt tacccagccc aacaatctcc ccacatattt catggcctaa
     5101 aacacgtgga taactaaaaa atggttgatt tccaccccag gcatgaatat cggtaccgca
     5161 aatcccgaca gacttaattt ttattaatgc ttcattgtca cccggaatag gtatctcgcg
     5221 ttgtttccag actaattctt tcggctgctg gcaaattaaa acattcatcg tagacatgat
     5281 tcggctctcc attttttgtt gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg
     5341 gaaaatctgt tttgtgaaaa cacacgcata aaaatgtttt aaatcgggtt ttaatggact
     5401 cacaaggagg aaaatatgag tcgttcacaa aatttacgcc acaatgtgat taaccaggtc
     5461 attgatgata tggcgcgggg tcatatccct tccccacttc catcgcaaag tgcgctggcg
     5521 gaaatgtaca acatcagccg tactacagtg cgtcatattc tcagccactt acgcgaatgc
     5581 ggcgtcttaa cgcaggttgg aaacgactat gttattgcgc gtaaacctga ccatgatgac
     5641 ggtttcgcct gtactaccgc ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt
     5701 ttcacgatga taaaccagcg ccagttacgc ccaggggaaa cgttttctga actgcaactg
     5761 gctcgggccg cgggtgtcag cccggttcta gtaagagaat atcttttaaa attcggccgt
     5821 tataatctta ttcacagcga aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc
     5881 tacgccgagc agctgtttga gctacgcgaa atgctcgaaa cacattcact tcagcacttt
     5941 ctcaacctgc ctgatcatga tccccgctgg ctgcaggcga aaaccatgct cgaacgtcat
     6001 cggctactgc gtgacaacat cggcaatagc tttcgcatgt tctcgcaact tgacagagat
     6061 tttcactcct tactgctttc agctgccgac aatatctttt ttgatcaatc gcttgagatc
     6121 atctcagtca tctttcattt tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat
     6181 atcattgccg tcgatgagca tatgaccatt ctcagcgctc tgatttgccg cagtgacctt
     6241 gatgccaccc ttgcgctccg taaccatttg aattcagcta aacaatcaat gattcgatcc
     6301 attaatgaga acacgcgtta tgctcattaa gtacggatta aaaacagcaa actattagcg
     6361 ggtgcacggt tttatcaaaa gataaatacc gggaaattcc cgcttaccta tgctcacaat
     6421 cagtcgacta aaaatgtttt tgtgagcggt agtaaagtcc taaaacttta acctgccgtt
     6481 aatgtcaacc ttgcgtaatg cacaattacg taaacaatta aaattataaa tacaggagtc
     6541 cggcgtggaa aaagaaaata tcaccctcga tccgcgttct tcatttactc catcttcgtc
     6601 ggcagatatt cccgtgccac cagatggatt agttcaacgc agtacccgaa ttaaacgcat
     6661 tcaaaccacc gccatgttgt tattattttt tgcggcggta atcaattatc tcgaccgcag
     6721 ttcgctgtcg gtagcaaatt taacgattcg tgaagaattg ggattaagtg ccaccgaaat
     6781 cggcgctttg ctctccgtgt tttcactcgc ttacgggatt gcgcaacttc cttgcggccc
     6841 actattggat cgtaaaggcc cacgcctgat gctgggactg gggatgttct tctggtcact
     6901 gttccaggca atgtctggca tggtgcacaa ctttacgcag ttcgtgttgg tgcgtatcgg
     6961 tatggggatt ggtgaagcgc cgatgaaccc atgcggtgta aaagtcatta acgactggtt
     7021 caacatcaaa gagcgcggac gcccgatggg cttcttcaac gcagcttcta ccattggcgt
     7081 tgccgtaagc ccaccgattc tggcggcgat gatgctggtg atgggctggc gcgggatgtt
     7141 tattaccatt ggtgtactgg ggatttttct cgccatcggc tggtatatgc tctatcgcaa
     7201 ccgcgagcac gtagaactga ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa
     7261 tgcccgccga gatccgctca gttttgccga atggcgcagc ctgttccgta accgtacaat
     7321 gtggggaatg atgctcggat tcagtggtat caactacact gcgtggctgt atctggcctg
     7381 gcttcctggt tacctgcaaa cagcctataa cctggattta aaaagcacag ggttgatggc
     7441 ggctatccct ttcctgtttg gggctgccgg gatgctggtc aacggttacg ttactgactg
     7501 gctggtcaaa gggggaatgg ctccgattaa aagccgtaag atctgcatta ttgccgggat
     7561 gttctgttct gccgccttta cgctggtcgt accacaagcg acaacatcca tgacggcggt
     7621 tctgctgatt ggcatggcac tgttctgtat tcactttgcc ggaacatcct gctggggctt
     7681 gatccacgtc gcagttgctt ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc
     7741 cagcttcatc tgcgcctctt ttgcgccgat cattactggt tttattgttg ataccaccca
     7801 ctcattccgt ctggcactaa tcatctgcgg ttgcgtcacc gcagcggggg cactggcgta
     7861 catcttcctg gttcgtcagc cgatcaacga cccacgtaaa gattaatctc cttatgcccg
     7921 ataacatttt gcttatcggg cattttcatg gcgattaaaa tgtttcccag ttctcctcgc
     7981 tatctgccac ggccatttta cgcggcgcag ctggcgtcac ggtttttacc acagccgatg
     8041 tttcacgctg ctgttgctgc tgttgctgaa tccggaacac tgccacagct tcggtcaggc
     8101 gactggcctg ctcttccagc gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt
     8161 tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg ataccacggc
     8221 tctgctcatc agaagcagaa gcaatttcgc ccataatgtc cgtcacgcgg gtcacggcgc
     8281 tgacaatctc cgccattgtt tccccggcgc tttcgaccag cgtagagcca acatccactt
     8341 tccccaccga gtcttcaata aggcttttaa tttcacgagc cgcctgagcg ctacgctggg
     8401 ccagattacg cacttctccc gcaaccaccg caaaaccgcg cccttgctca cccgcacgcg
     8461 ctgcttcaac cgccgcgttc aaagccagaa tattggtctg gaaggcaatg ccgtcaatta
     8521 cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcata gtctgcacca
     8581 cgttatctac cactttgccg ccccgttgcg ccgtttcaga agcacttaat gccagatggc
     8641 tggcctggcg cgcattctcg gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg
     8701 ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga tcgttattgc
     8761 cggtagcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc acatcaccga
     8821 cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc cccatctcat
     8881 tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga cggatgctgt
     8941 caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag acggcgaaga
     9001 tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg ctgtaggagg
     9061 cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg taagccacat
     9121 actgcttctc gaaaccgtct tgatatccct gggtcggctg atcaaagaac tcgttgattt
     9181 tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata tcgtaattac
     9241 gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat
     9301 ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc agctcagcaa
     9361 cggttgaacc gctaccaata ttattctgat ccatcatgta gcggataccc gcgcggttga
     9421 gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtggat tgctgctggc
     9481 gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta aagaacagac
     9541 cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag ctggtcacga
     9601 tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat tttcggcctg
     9661 gaaagcaaaa actttatgaa aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt
     9721 tatcagatac taaaaagtta ttcattatcg cccgggatgg cttcttgtcg ggaattgccc
     9781 agagcaataa attcctctaa cagatgggtt aactgttgca ttttctcggc agtaaactga
     9841 gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc
     9901 ccctctttgg ttagcgagat atagagcttc cgctgatcat tgatcggctt taatcgcaac
     9961 actaaaccgt cgcgttccat ccgcgtaagg atcccggtca ggcttgggcg caaaatgcat
    10021 gcgcgatacg ccagatcgtg aaaatccatt gatgggcttt ccgccagaat acgcacgatg
    10081 cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata actcatcgcc
    10141 gcttctcgcg cctggagcaa cgcaatggtt agagagtcgt gcattatctt tcccctgaaa
    10201 aattcaaaat cattaatata gaaacagtta atcatcacgt taccgggact gcgctgcccc
    10261 tgcaattctt cattttatca aaattgagca tgttttgtta acttgctgat atttaatatt
    10321 ttattatctt taaatgtaaa tagtttgtta attagatcac atttacatca cttacttttg
    10381 ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta aaataatcga
    10441 atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca tcgcagccaa
    10501 cttgatgcat ggcaggaagc gttccagcaa tccccctaca aagccccgcc taaaactgcg
    10561 gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca
    10621 cagggtgaaa aggtactgag cggtgcgact gttgcgctga ttgtgggaaa aacggcgacg
    10681 aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc
    10741 agcctgccgg aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg tgatggattc
    10801 tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgaccat ctataccgag
    10861 atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcgcag
    10921 ttgctgagcg ccctgagcga atttgccacg ctgaatccag gcgacgccat tctgctcggc
    10981 acgccacagg cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct cgcagaaggt
    11041 ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc
    11101 ccaacgcagc cacacccgca cggcacgctg tttgccctcg gcctgaacta cgccgaccac
    11161 gccagcgaac tggaatttaa gccaccggaa gaaccgctgg tgttcctgaa agcgccaaat
    11221 accctcactg gcgataacca gacctccgtg cgtccaaaca atattgaata catgcactat
    11281 gaagcggagc tggtggtagt tattggcaaa caggcgcgta acgtcagcga agccgatgcc
    11341 atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga ctatctggaa
    11401 aactactacc gccctaacct gcgggtcaaa agccgcgacg gactgacgcc gatgctttca
    11461 accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc
    11521 aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt gcccttcctg
    11581 atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca
    11641 ccaaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg
    11701 ggccgcctgg tgaaccgaat tgtgagtgag gatacagcga aatgaaaaaa gtaaatcatt
    11761 ggatcaacgg caaaaatgtc gcaggtaacg actacttcca gaccaccaat ccggcaacgg
    11821 gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg
    11881 cagcgaaaga ggcgttcccg aaatgggcca atctgccgat gaaagagcgt gcgcgcctga
    11941 tgcgccgtct gggcgatctg atcgaccaga acgtgcctga gatcgccgcg atggaaaccg
    12001 ccgacacggg cctgccaatc catcagacca aaaatgtgtt gatcccacgc gcttcccaca
    12061 actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacttat ccggtcgacg
    12121 acaagatgct caactacacg ctggtgcagc cggtaggcgt ttgtgcactg gtgtcaccgt
    12181 ggaacgtgcc gtttatgacc gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata
    12241 ccgcggtact gaaaatgtcg gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg
    12301 cgctggaagc cggtattccg gcaggcgtgc tgaacgtggt acagggctac ggcgcaaccg
    12361 caggggatgc gctggttcgt catcatgacg tacgtgccgt gtcgttcacc ggcggtacgg
    12421 ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg gaactgggcg
    12481 gtaaatcgcc ggtgctgatt tttgaagatg ccgatattga acgcgcgctg gacgccgccc
    12541 tgttcaccat cttctcgatc aacggcgaac gctgcaccgc cggttcgcgc atctttattc
    12601 agcaaagcat ctacccggaa ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg
    12661 tgggcgatcc gaacgatccg aatacccagg ttggcgcgct tattagccag cagcactggg
    12721 aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg ctggcgggcg
    12781 gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa
    12841 ccgtgctggc agacgttgat aaccgtatgc gagtcgccca ggaagagatt ttcgggccgg
    12901 tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgtctggca aacgacgtgg
    12961 agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta cgcctggcgc
    13021 gtggcattga agctggcatg gtgttcgtca acacccagaa cgtgcgtgac ctgcgccagc
    13081 catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag
    13141 tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt ccgaaatggg
    13201 gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc gtcgatgtat
    13261 ctctctgaac tgccagggaa aaaccacggt tgccgccagg gcgcgatcga cgggcataaa
    13321 gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt cgatacccac
    13381 tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg cgtctacacc
    13441 agcaacgagc tgccgcattt tatccgcgat atgacctaca actacgaggg caacccggag
    13501 ttggggcagc ttattgccga tgaagcctta aagctcggcg tgcgggcaaa agcgcacaac
    13561 attcccagcc tgaaactgga atacggcacg ctggtgccaa tgcgctacat gaatgaagac
    13621 aagcacttca aagtggtttc catttcagct ttctgcactg ttcacgattt tgccgacagc
    13681 cgcaagctgg gcgaggcgat tctgaaagcg atcgaacagt acgacggcac cgtggcggtc
    13741 cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgagcgga agaagggatg
    13801 aacagctaca cccgcgagtt cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc
    13861 gaaggccagt tcaaagagtt ctgcaatatg ctgccggagt acgccgacta ctgctacggc
    13921 gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga taaatacgac
    13981 ggcaaggtgg agtttatcac cgagctattc ccaagctctg gcaccggtca ggttaacgct
    14041 gttttcccgc ttcccgcgta aggacgtttt atgccgcact ttatcgttga atgcagtgac
    14101 aacatccgcg aagaagccga cctgccgggg ttgttcgcca aagtgaatcc gacgctggca
    14161 gccacgggta tttttccgct ggcgggtatt cgcagccgcg tgcattgggt cgatacctgg
    14221 cagatggccg acgggcagcg tgattatgcc ttcgtgcata tgacgttgaa aatcggcgca
    14281 ggtcgcagcc tggaaagccg tcagcaggcg ggtgaaatgc tgtttgaact gattaaaacg
    14341 cacttcgccg ccctgatgga gagccgcctg ctggcgttgt cgtttgagat tgaagagctg
    14401 catccgacgc tgaattttaa acaaaacaac gtgcacgcat tgtttaagta gcgcgcagat
    14461 tgcccggtgg cgctgcgctt accgggccta caaaacccca aaccgtacac cgtaggccgg
    14521 ataaggcgca gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacataccc
    14581 acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga
    14641 tctcgctgga ttacccggag atcaccatcg aagacgctta cgcggtgcag cgtgaatggg
    14701 ttcgcctgaa aatagccgaa ggtcgcacgc tgaaaggcca caaaatcggc ctgacctcga
    14761 aagcgatgca ggccagctca caaatcagcg agccggatta cggtgccctg ctggatgaca
    14821 tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag
    14881 tggagctggc tttcgtgctg gcaaaaccgc tgcgtggacc aaactgcacg cttttcgacg
    14941 tctacaacgc cacggactac gtgatcccgg cgctggagct gatcgacgct cgctgccaca
    15001 acatcgatcc ggaaacccag cgcccgcgca aagtgttcga caccatttct gataacgccg
    15061 ccaacgccgg ggtgatcctc ggtggtcgtc ccattaagcc cgatgagttg gatctgcgtt
    15121 ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg
    15181 tgctaaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg
    15241 tacaactgga agccgggcaa atcattctcg gcggttcgtt cacccgcccg gttccggcgc
    15301 gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg
    15361 tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgcccgcag
    15421 atcggattat ggctggggct gagtagcagc tacagcgcag agttactggc cggagcagga
    15481 ttcgactggt tgttgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc
    15541 cagctacagg cgattgcgcc ctaccccagc cagccggtag tacgtccgtc gtggaacgat
    15601 ccggtgcaaa tcaaacaact gctggacgtc ggcacacaaa ccttgctggt gccgatggta
    15661 caaaacgctg acgaagcccg tgaagcggta cgcgccaccc gttatccccc cgccggtatt
    15721 cgcggtgtgg gcagtgcgct ggcccgcgcc tcgcgctgga atcgtattcc tgattacctg
    15781 caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag
    15841 aacttaccgc agattctgga cgtggaaggc gtcgacggcg tgtttatcgg cccggcggat
    15901 ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt
    15961 gagcaggcga tcgtgcagat ccgtgaatcg ggcaaagcgc cggggatcct gatcgccaat
    16021 gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac
    16081 accaccctgc tcgcccgcgc cgctgaagcg ctggcagcac ggtttggtgc gcaggccacc
    16141 gccgtgaagc ccggcgtgta ttaatgccgg atgcgctgca cttatccggc ctacactcgc
    16201 accaaaccgt aggccggata aggcgtttac gccgcatccg gcaaaaagcc gtaccaaatc
    16261 gcggaccgga taaggcgttt gtgcgcatcc gccataaacc ttgtcgtacc ctacaaaaat
    16321 cccattagag gaagaaaaat gagcgacacc tcacctgcca taccggagag tatcgatccg
    16381 gcgaatcagc ataaagcgct gactgccgga caacaggcgg ttattaagaa gctatttcgc
    16441 cgcctgatcg tctttctgtt cgtgctgttt atcttctcgt tccttgatcg catcaacatc
    16501 ggctttgccg gactcacgat gggacgcgac ctcggtctga gcgccaccat gtttggcctc
    16561 gctaccaccc tgttctatgc cgcttatgtc atcttcggca ttcccagcaa cattatgctg
    16621 agtattgtcg gtgcgcggcg ctggatcgcc accatcatgg tgctctgggg catcgcctct
    16681 actgccacca tgtttgccac tggccccacc agcttgtacg tactgcgtat actggttggc
    16741 attaccgaag ccggctttct gcctggtatt ctgctgtatt taaccttctg gtttccggcc
    16801 tatttccgcg cccgtgccaa cgccttgttt atggtagcaa tgccggtaac gacagcgttg
    16861 ggctcgatcg tttccggcta cattttgtcg ctggatggcg tcatggcatt aaaaggctgg
    16921 cagtggctgt ttttgctgga aggcttcccg tcggtattac tcggcgtcat ggtgtggttc
    16981 tggcttgatg actcaccgga caaagctaag tggctgacga aagaagacaa aaaatgcctg
    17041 caagagatga tggataacga tcgtctgacg ctggttcagc cagagggagc catcagccac
    17101 cacgccatgc aacaacgcag tatgtggcgg gagatcttca ctccggtggt gatgatgtat
    17161 accctagcgt atttctgcct gaccaacaca cttagtgcga tcagcatctg gacaccgcag
    17221 atcctgcaaa gttttaatca gggcagcagt aatatcacca tcggcctgct ggccgccgta
    17281 ccgcagattt gtaccattct cgggatggtc tactggagcc gtcactcaga tcgccgccag
    17341 gaacgaaggc atcacaccgc ccttccttat ttgttcgctg ccgcaggttg gttactggct
    17401 tcggcaactg atcacaacat gatccagatg ctggggatca ttatggcttc gaccggatca
    17461 ttcagcgcaa tggcgatttt ctggacaaca ccggatcagt ccatcagcct gcgggcacga
    17521 gcgatcggta ttgcggtgat caacgccact ggcaacattg gttcagcgtt aagtccgttt
    17581 atgatcggct ggttgaaaga tttgaccggc agctttaaca gtggattgtg gtttgttgcc
    17641 gcgctgctgg tgattggtgc ggggattatc tgggcgattc caatgcagtc ctcccgtccg
    17701 cgagcgaccc cgtaaggaac gacgatgtgt gaccgtcaga ttgccaatat tgatatcagc
    17761 aaagagtacg atgaaagcct gggcacggac gatgtgcatt atcagtcgtt cgcccgcatg
    17821 gcggcctttt ttggccgcca tatgctgcca catcgccacg aacagtactt tcagatgcat
    17881 ttcctcaata gcggacagat tgagctacag cttgacgatc atcgctactc ggtggaagcg
    17941 cccctgtttg tcctgacgcc gccgtcagta cctcatgcgt ttattacgga gtctgatgct
    18001 gacggtcatg tattgacggt acgggaagat ctgatctggc ccctgctgga agttctttat
    18061 ccgggcactc gggaaacctt cggcctgccg gggatttgcc tgtcactggc agataaaccc
    18121 gacgaactgg cggcgctgga acactattgg caactgatag agcgggaatc ggtagaacaa
    18181 ctgcctggat gggaacacac cctgacgtta ctggcacagg cagtgttcac cctactgctg
    18241 cgtaacgcaa aactcgacga ccatgccgcc agcggaatgc gcggagaatt aaaactgttc
    18301 cagcgttttc atatgcttat tgaaagccat tttcatcagc actggacagt accggattac
    18361 gctaacgaac tgcatatcac cgaatcacgc ctcacggaca tctgccgccg ctttgccaac
    18421 cgtccgccaa aacggttgat tttcgacagg cagctacgag aagccaagcg gctgctgctg
    18481 ttttctgata acgccgtgaa caatattgcc tggcaactcg gttttaagga tccggcttat
    18541 tttgcgcgct tttttaatcg cttagtcggt tgctcgccca gtgcttatcg tgccaaaaaa
    18601 gtacctgtga cgtgaaattc ccttagcctt aacgggaaac caggcaccac ctgctattcc
    18661 cctttcttcg cctggagcaa ggggatttag taacgttaaa aatcgctaaa agcgacctcg
    18721 atcacaaaac cgtacccgac ctgaaaagta ccagccattg tcccaaaagt gtcttcccgt
    18781 aatcgcccct gcaagcttca attgaataac aaaaacaaca cacaaaatta acaatcgtat
    18841 tccgattaat actgtagagg tcgacatgaa accagaagat ttccgcgcca gtacccaacg
    18901 tccgttcacc ggggaagagt atctgaaaag cctgcaggat ggtcgcgaga tctatatcta
    18961 tggcgagcga gtgaaagacg tcaccactca tccggcattt cgtaatgcgg cagcgtctgt
    19021 tgcccaactg tacgacgcgc tacacaaacc ggagatgcag gactctctgt gctggaacac
    19081 cgacaccggt agcggcggct atacccataa attcttccgc gtggcgaaaa gtgccgacga
    19141 cctgcgccag caacgcgacg ccatcgctga gtggtcacgc ctgagctatg gctggatggg
    19201 ccgtacccca gactacaaag ccgctttcgg ttgcgcactg ggcgcgaatc cgggctttta
    19261 cggtcagttc gagcagaacg cccgtaactg gtatacccgt attcaggaaa ctggcctcta
    19321 ctttaaccac gcgattgtta acccaccgat cgatcgtcat ttgccgaccg ataaagtgaa
    19381 agacgtttac atcaagctgg aaaaagagac tgacgccggg attatcgtca gcggtgcgaa
    19441 agtggttgcc accaactcgg cgctgactca ctacaacatg attggcttcg gctcggcaca
    19501 agtgatgggc gaaaacccgg acttcgcact gatgttcgtt gcgccaatgg atgccgatgg
    19561 cgtgaaatta atctcccgcg cctcttatga gatggtcgcg ggtgctaccg gctcgccgta
    19621 cgactacccg ctctccagcc gctttgatga gaatgatgcg attctggtga tggataacgt
    19681 gctgatccca tgggaaaacg tactgatcta ccgcgatttc gatcgctgcc gtcgctggac
    19741 aatggaaggc ggttttgccc gtatgtatcc gctgcaagcc tgtgtgcgcc tggcagtgaa
    19801 actcgacttc attacagcac tgctgaaaaa atcactcgaa tgtaccggca ccctggagtt
    19861 ccgtggtgta caggccgatc tcggtgaagt ggtggcgtgg cgcaacacct tctgggcatt
    19921 gagtgactcg atgtgttctg aagcgacgcc gtgggtcaac ggggcttatt taccggatca
    19981 tgccgcactg caaacctatc gcgtactggc accaatggcc tacgcgaaga tcaaaaacat
    20041 tatcgaacgc aacgttacca gtggcctgat ctatctccct tccagtgccc gtgacctgaa
    20101 caatccgcag atcgaccagt atctggcgaa gtatgtgcgc ggttcgaatg gtatggatca
    20161 cgtccagcgc atcaagatcc tcaaactgat gtgggacgcc attggcagcg agtttggtgg
    20221 tcgccacgaa ctgtatgaaa tcaactactc cggtagccag gatgagattc gcctacagtg
    20281 tctgcgccag gcacaaagct ccggcaatat ggacaagatg atggcgatgg ttgatcgctg
    20341 cctgtcggaa tacgaccaga acggctggac tgtgccgcac ttgcacaaca acgacgatat
    20401 caacatgctg gataagctgc tgaaataacg cagcaggagg ttaagatgca attagatgaa
    20461 cagcgcctgc gctttcgtga cgcgatggcc agcctgtcgg cagcggtaaa tattatcacc
    20521 accgagggcg acgccggaca atgcgggatt acggcaacgg ccgtctgctc ggtcacggat
    20581 acaccaccat cgctgatggt gtgcattaac gccaacagtg cgatgaaccc ggtttttcag
    20641 ggcaacggta agttgtgcgt caacgtcctc aaccatgagc aggaactgat ggcacgccac
    20701 ttcgcgggca tgacaggcat ggcgatggaa gagcgtttta gcctctcatg ctggcaaaaa
    20761 ggtccgctgg cgcagccggt gctgaaaggt tcgctggcca gtcttgaagg tgagatccgc
    20821 gatgtgcagg caattggcac acatctggtg tatctggtgg agattaaaaa catcatcctc
    20881 agtgcagaag gtcacggact tatctacttt aaacgccgtt tccatccggt gatgctggaa
    20941 atggaagctg cgatttaagc tccagcccct tcacgcattc gtcgaagggg catttttatt
    21001 gtgatctcaa tcacatatcc acccgatgaa taattcttgc ccaaccgctc accgccattc
    21061 ctgaccgctt acgtccctaa aataaccact cagttattta ccttacttta cgcgcgcgta
    21121 actctggcaa catcactgca ggatagcggt caatttacct cctcaaacgc aacgcaaacc
    21181 tagaacggct tcggcctatg attaacaaat tactctatga attttggttt gcaggaaggc
    21241 agcaagtgag tgaatccccg ggagcttaca acagtaagtg acaggggtga acgaacgcaa
    21301 ctgccgcacc tgtaagccaa aagacgacga gtaaaatgcc aggttttact atggatacga
    21361 aaaaactatt caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg
    21421 cggtagttgc attacgtcgg ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg
    21481 tgtcggtgta tctggtggcg tatcgctact acagtctgta catcgcccag aaggtgatga
    21541 aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactacgttc
    21601 cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc
    21661 tggtcggtcc ggttctcgcc gcacagatgg gctacctgcc tggcacgctg tggctgctgg
    21721 cgggggtagt gctggccggt gcggtacagg actttatggt gctgtttatc tcctctcgcc
    21781 gtaacggcgc atctctcggt gagatgatca aagaagagat ggggccagta ccggggacta
    21841 tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct tgccgtcctg gcgctgattg
    21901 tggttaaagc cctggccgaa agtccgtggg gtgtcttcac cgtttgctca accgtaccga
    21961 ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg ggtgaagtct
    22021 ctgtcattgg tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg
    22081 atccgtactg gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg
    22141 gctatgcgtt tgtttccgca ttgctgccgg tgtggctgat cctcgcaccg cgcgactatc
    22201 tggcaacctt cctgaaaatc ggcgttatcg tcggcctggc gctgggtatc gtggtgctga
    22261 acccggaact gaaaatgcct gcgatgaccc agtacattga cggtactggc ccgctgtgga
    22321 aaggtgctct gttcccgttc ttgttcatta ccatcgcctg tggtgcggta tctggcttcc
    22381 acgcactgat ctcttccggt acgacgccga aactgctggc taatgaaacc gacgcgcgtt
    22441 tcattggcta cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgctg
    22501 cgtccatcat cgaaccgggt ctttacttcg cgatgaacac cccgcctgct ggccttggca
    22561 tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcgcagc
    22621 tgaaagacgt taccgcacac gcggcagcga ccgtcagctc ctggggcttc gtgatttcgc
    22681 cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag
    22741 gtggtgcgcc aacgctggcg gtaggtatcg ctcacgtgtt ccacaaagtg ctgccgatgg
    22801 ctgacatggg cttctggtat cacttcggta ttctgttcga agccctgttc atcctgaccg
    22861 cgctggatgc gggtactcgt tctggccgct ttatgctgca agacctgctg ggtaacttca
    22921 tcccgttcct gaaaaaaacc gactctctgg ttgccggtat catcggtact gcgggctgtg
    22981 tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga
    23041 gcctgtggcc gctgttcggt atttccaacc agatgctggc agccgtagcg ctggtactgg
    23101 gcaccgttgt gctgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg
    23161 ctgtatggct gcttatctgc accacctggg cgctgggcct gaaactgttc agcaccaacc
    23221 cgcagatgga aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta
    23281 ctgacctgac ggcgcagcag attgccaaca tgaaccacat cgttgtaaac aactacacca
    23341 acgcaggcct gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca
    23401 aaacctggct tgcggtgcgt aacagcgaca aacgtactga caaagaaact ccgtacgttc
    23461 caatcccgga aggcggcgtg aagatctctt cgcaccacta accgtgttta gccccgcttc
    23521 ggcggggctt tgttcttatc agagtgaact atgtttggta acttaggaca ggcaaaaaaa
    23581 tatctcggcc aggcggcgaa gatgttgatt ggcattccag actatgacaa ctacgtcgag
    23641 catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt cttccgcgaa
    23701 cgccagaacg cacgctacgg cggcgatggt aaaggcggta tgcgctgttg ttaatggaga
    23761 gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg gcaaaaccac
    23821 cctgctgcgc catattctta atgaacaaca cggctacaag attgccgtga ttgaaaacga
    23881 attcggcgaa gtctccgttg atgatcaatt gattggtgat cgcgcaacgc agatcaaaac
    23941 gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaggacg cgctactcga
    24001 cctgctggat aatctcgaca agggcaatat tcagttcgat cgtctggtca ttgaatgcac
    24061 cggcatggcc gatcctggcc cgattattca aacctttttc tcccatgaga ttttatgcca
    24121 gcgctacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg atgagcagat
    24181 gaaccagttc accatcgccc agtcccaggt gggctacgcc gaccgtattc tgctgactaa
    24241 aaccgacgtc gcaggcgaag cagaaaaact gcgtgaacgt ctggcacgca tcaacgcccg
    24301 tgcaccggtc tacaccgtca cccacggcga catcgacctg ggtctgctgt tcaacaccaa
    24361 cggttttatg ctggaagaaa acgtcgtcag caccaaaccg cgtttccatt ttatcgcgga
    24421 caaacaaaac gatatttcgt cgattgtggt ggaactgaat tacccggtag atatcagcga
    24481 agtttcccgg gtgatggaaa acctgctgct ggaatcggcg gataaactgc tgcgttacaa
    24541 agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg tccagcgcct
    24601 ctacagcgcc gactgggaca ggccatgggg cgatgaaaaa ccgcatagca cgatggtgtt
    24661 tatcgggatt cagttgccgg aggatgagat tcgggcggcg tttgcgggac tcaaaaagta
    24721 accagccctc caacatcaca atacctgaga ctttatcacc ctcatattcc tccagaatat
    24781 gagggctttc gggcttaatt atctctgaac gtgcaacact tcctgcgact ccttatgcgc
    24841 ctccacatcc tgcttcgtta accagagcga cttacggcca aaattttcgt acattttcag
    24901 ctgatcttca tagtgcttat caactgttcc atcgggagca ataaacccac tctgaccggg
    24961 tgcgaccaca tcccaggcaa gcacaggacg atcgcttgtc gttggtgaga aaacaatcat
    25021 atcgttttct gttccacggt tttgatactc cgcctgatga cgcgtttctt ccgctgcggc
    25081 ctgcggtaca ccaaagaaat tatttgcccg gaacgttaag gccattgcag gtgttttcca
    25141 gttactcaca ttattgccat agcgtttgga aagagtctcc caggtatctt ccagcgcagc
    25201 caacacaacc tcctgctgtg gtttcccagc aaacagatca accgcctgtg ggattggtga
    25261 tttgtctccc tgcaccgcct catacaaaat ttttgctcca acacttatat tcagcgaacc
    25321 agttgggccg tcctgggttg tttcgtagcc actggcgctg taccacttat caaatggcat
    25381 aggtacggca gccactacgg tacgcttcaa catactggtc agccaaacgt tcaggatggc
    25441 agagcctggc tgctgccagg ttttaccatc atcattaagc aaattgatgc catcccaacg
    25501 tgttaatgtt tctaccaact gacgacgcgg atcgctctgt gtcaaaccag atgtcgctgc
    25561 ttgcagagta ggtaaaaaaa gcctcaggtt aagatcctga cgactggttt ggcgaataac
    25621 atcccatgcc tgatcagcag ttaagcgtgg cttttgctca agcagtcggt cgatctccgt
    25681 aacgcgatct gcaccacccc acaaaaaggc aaacagatct gaagcgggat aatctttttg
    25741 gggagaattg ttccagttag caatatatcc cgactggggg ttatacacct tagggttcat
    25801 ttcaaaaggc aatagccctt tccagtccca ttttcccgta ccaggaacgg gtaatcgcgg
    25861 atcatggcct gattgacgat ctggataagc accagtatga acataaccaa tattgccgtt
    25921 tacatcagca tagtaccagt tgatggtcag tgcttgtttc gctgcctgct gtgtccactc
    25981 ctgccaattt ttggccttca tctgatgagt ccaggccagc aaagacgcca cctctttacc
    26041 atcccatgcg cgggatttag cgtaagccgt ttgtgtcgtc tggtcagttt ggagaatgtt
    26101 gccatgcacc gtacgccaga cagtaaaggt ctctgcctga ccatttttca ccgtaatggt
    26161 ttcctcacgg cttaacattt tcacccactt accattatgc aagtagtagc ctggtttctc
    26221 tgccgacagc cgttcagcaa aaatatcgac atcatcgccg aaacctgccg ttgatcccca
    26281 ggaaatcaca ccattatgac caaaaaccag cccaggatag gcaaatggtg tattgccagt
    26341 gacatcataa ccagcaccgt gcagaccaat accataagta tacgcaggcg cataccagcc
    26401 aaactgcgga ccatttacca tgattgcttt cgcatcctgg gctttgcttt tgccgatcac
    26461 ccacatattg ctggtcgttg gataccccgc cagaccattg gcaccaccct gtgcaaattg
    26521 tgcagcaata gtttcccggt tcttccctgc tgttaacgcc agcagtgcgc catccgcccc
    26581 ttttgctggt cggtcaagca ttggtgcagg taaatcgtag cgtggcaaca gagctgctgt
    26641 ttgcgagttt tgctgattaa atttaagtgg gtagttactc tcttgtacgg caatagtggt
    26701 tggcgctgat gggtttacca gccatttcaa ctgattaaat accgccatgc cttgtgatac
    26761 accatattta tcttttaaag ccgttagcag tgccagatta tcaatttcgc tagtgctatc
    26821 agagaagcgg tttgccatgg tgcccacaaa tatcatcgcg acatcaaacg gttcccagcg
    26881 cttaggagta aagccaaatg tattaaactg ttttggtaag agcgtctctg gattggtatt
    26941 taccttatca atccaggcat tcattccatc agcgtagcct tgcagaatgg acatatcctc
    27001 tggggaaagg gcagcaattt gcgcccggat agcatccggc cagtagttac gacggatatc
    27061 tttatcaaat ttcacaaaat ctttgccaag cacttccgcg acagtccctt gagtactgcg
    27121 acgtgccatt tccatctgaa aaaggcgatc ttgtgctact acatagccat agccataaaa
    27181 taggtgccat gtatcattgg cataaatatg cggcatgccg tattcatcgc gaacaatctt
    27241 tatctcactt gacgactgct cagccagtgc aggtaagctc caataataca tcagggaagc
    27301 agtaacacag ttcacgatca tacgatttct atttttcatt gtatcctctg gcaggtgtat
    27361 aattagcgaa ttgatactag caacgaagct tcggttatct atttgcagag aattgtttca
    27421 ttatgaactg tgagcgcgat aactaatttt tgatacaaaa atttcattaa tgtgatctaa
    27481 aacgaatcgc tttattcacc gctgatgatt ttgttctatt cctttttgtg acagatcccc
    27541 cggacgcata gatgatgacc cccagagaga cagctcaggt cattatctgt atgtaaatca
    27601 gaaaaatatg ttgtaaacga cttttatggt tggattttgt agcaattttg aatgcagaca
    27661 ggcagcattt gccaacacgc cgcccatagt tcactccaca tcctccctgc gtaacccaat
    27721 atccctcaac cgttcatcac tcatctgctg taacactcgt cgggtctgca tttgccgccg
    27781 ccaacgcctc accgcatgcc agagttgcac aagtctgata aacggcgttt tggctttgtt
    27841 ttcgtgaaat tccatctgct gcctcctcat ctttcagagg cttcatcatc cgccttctgg
    27901 cagttcacaa tacagatgca aatattagtt ttatttaaca tacagacagg ttaaaacagc
    27961 atctgcatcc ggttttttag cctcatctgt accggttttt ctttctgtat gggccaaata
    28021 aggaatacag catgacgcgt tatcaacatc tggcgactct acttgccgag cggattgagc
    28081 aagggctgta tcgtcacggg gagaaattgc cgtcggtgcg cagcttaagt caggagcacg
    28141 gcgtcagcat cagcaccgtg caacaggcgt atcagacgct ggaaacgatg aggctcatca
    28201 ctccgcagcc gcgttcgggt tattttgtcg cacaacgtaa agcccagccg cctgtaccgc
    28261 cgatgacgcg tccggtgcag cgcccggtgg aaattaccca gtgggatcag gtgctggata
    28321 tgctggaggc gcatagcgac aattccattg ttccgttaag caaaagcacg ccggatgtcg
    28381 aaacgcccag cctgaaaccg ctgtggcgtg agctaagccg ggtggtgcag cataatctgc
    28441 aaaccgttct cggttatgac ttgttagccg gtcagcgagt gttgcgggag cagattgccc
    28501 gcctgatgct cgacagcggc tcggtggtca ccgccgatga catcatcatc accagcggct
    28561 gccataactc gatgtcgctg gcgttaatgg cggtgtgtaa accgggcgat attgtcgcgg
    28621 tcgaatctcc ctgttattac ggttcgatgc agatgctgcg aggcatgggc gtgaaagtga
    28681 ttgaaatccc aaccgatcca gaaactggca tcagcgttga agcactggaa ctggcgctgg
    28741 aacagtggcc gattaaaggc atcattctgg tgccaaactg taataatccg ctgggattta
    28801 ttatgccgga cgcacgcaaa cgggccgttc tctctctcgc tcagcgtcat gatattgtga
    28861 tttttgaaga tgatgtctac ggcgaactgg caacggagta tccgcgcccg cggaccatcc
    28921 attcatggga tatcgacggg cgagtgttgt tgtgcagctc gttcagtaaa agtattgctc
    28981 caggcctgcg cgtgggttgg gtcgcaccgg ggcgttatca cgataaactg atgcatatga
    29041 aatacgccat cagcagcttt aatgtgccgt ccacgcaaat ggcggcggca acgtttgtgc
    29101 tggaaggtca ctatcatcgc catatccggc ggatgcggca gagctatcag cgcaatttgg
    29161 cgctttatac ctgctggata cgggaatatt ttccctgcga aatctgtatt acgcgcccga
    29221 aaggcggatt tttactgtgg atagaattgc ctgaacaggt cgatatggtc tgcgttgcgc
    29281 ggcagctgtg ccgcatgaat atccaggtgg cagcaggctc gattttctcg gcttccggca
    29341 aataccgtaa ttgtctacgc atcaactgcg ctttgccgct cagcgaaacc tatcgcgaag
    29401 cactaaagca aattggcgag gccgtgtatc gggcaatgga ataaattcga attagtggcg
    29461 aagcacctga tttcctctca cgaatcgcct gtaactcgtt gtatctttgc agggtcatcg
    29521 ccacgttcca gcatggctta ggcaaagtgt gcttcgttca taccggatgc ggcgtgaacg
    29581 ccttatccgg cctacaaacc gcgcaaattc aatagattgc agaggaaatg taggtctgat
    29641 aagcgtagcg catcaggcag ctttgcgttt gtcatccgtc tctgattagt attcgatcca
    29701 tggatcgcgc ttgatttcag gcaagggctg ctctaatgcc tgcatttgct ctgtcgaaag
    29761 tccggtaatc ttttgtatcc attcaggatc ggccccattc tgcaacaaca atctaaggag
    29821 gcgttgttcc ccttcttctt tccccttttc catccccaac agccatccat cattataaat
    29881 tcgctctgca atcgtcatta ttcgctccct gtgttgtggc attcgacggg taagttcgct
    29941 gataaacgcc ttaaaacgcg cttcatcgcc agtcagtaaa atgtaattta acagcgcagt
    30001 tatctggctg tcattagcac actctgtaac cagtaatact accagttggt caataagccc
    30061 catcagatcg cgctggcgaa tatgcttttg gatcaactcc aacagggcga ctctgcgatg
    30121 ctgcacaatc tcgtcgtctg gcacgacagt gacatccacc agcgggaacg ctgcgctata
    30181 aagtttccgt gcggtagtcg gggcggcaaa ttcgtccagc cagcacaggg accaggggta
    30241 aggactacgg ctaccgtgat aaaacagcat cgggataacc agcggtagcg gccggcgttt
    30301 atcatgctcg atatggcgct gcatcaccgc catggaatat cgcatcaggc gaaacgccat
    30361 atgaatatcc tcgcggctct gatgttcaat cactacgtaa atataaccat caccttcacg
    30421 ggtctttacc gaccacagaa tatcggagtg tagcgcccgc aatttttcat cgacaaagct
    30481 ggcggattcc agttttaagc tgtcgagatc gcacagttca cgtaaatctt taggtaagtg
    30541 gatctccata aaatcccgcg cggtgccagg gtgcgtgaga aaggacttaa ataatgcgtc
    30601 atgcggcgtg ctggtcgaga agtttgtcat ggcgatccgt cacctgtaga taagatggcc
    30661 cgactttagc ggctaacaaa accattctca cccggcaatt tcccgttccc cgaggcgctt
    30721 tccaggctat ttttattctt ctgcaaacga tacatttttt cggaaacagg ctcgcgtttt
    30781 acatttcctg tacccggcag atattcccct ttccaccgcg ctcttttgat taaccgcaaa
    30841 ttttaattac acttaaggtg tatattttct atgcaaccca tcaattaaag aggtgtaatg
    30901 tgctgatgac tatttgtaat cgttatacat tctgacctgg agtcagaagg tatttctctg
    30961 tctgtgtgtt cacaggcagt gtggttgatt acatgaaatt cagtacattt gtccttctcg
    31021 cctgcctttc gtcattaccg acggtattga atttcgtttt ccccgttggg gttctccgga
    31081 caaggagttg tttgttatgc cacgtttttt tgcccgccat gccgccacgc tgtttttccc
    31141 gatggcgttg attttgtatg actttgccgc gtacctgtcg acggatctga tccagccggg
    31201 gatcattaat gtggtccgtg attttaatgc cgatgtcagt ctggccccgg cagccgtcag
    31261 tctctatctc gcgggcggta tggcgttaca gtggctgctg gggccgcttt ccgacaggat
    31321 tggccgcagg ccggtgctga ttaccggggc gctaatattt acccttgcct gcgctgcgac
    31381 aatgttcaca acgtctatga cacagtttct tatcgcgcgt gcaattcagg gcaccagtat
    31441 ctgttttatt gccaccgttg gttatgtcac ggtgcaggag gcgttcggcc agacaaaagg
    31501 gatcaagttg atggcgatta tcacctccat cgtactgatt gcgccgatta ttggcccgct
    31561 ttccggcgca gctctgatgc actttgtgca ctggaaagtc ctttttgcca tcattgcagt
    31621 tatgggtttt atctcatttg ttggcttact gctggcgatg ccagagacgg tgaagcgcgg
    31681 cgcggttcca tttagcgcca aaagcgtctt tcgcgatttt cgtaatgtct tttgcaatcg
    31741 gctgttcctc tttggcgcag caaccatctc tttaagctat atcccaatga tgagttgggt
    31801 ggctgtctcg ccggtgatcc tgatcgacgc gggcggctta acaacttcgc agttcgcctg
    31861 gacacaggtt ccggtgttcg gcgcggtgat tgtcgcgaat gccatcgtgg cgcgtttcgt
    31921 taaagatccg accgaaccgc gctttatctg gcgtgctgta cccattcaac tggtcggcct
    31981 cgcgctgttg attatcggca atctgctgtc accgcacgtc tggctgtggt cggtgctggg
    32041 caccagtttg tatgctttcg ggattggttt gatcttcccg accttattcc gctttacgct
    32101 tttttccaat aacttaccga aagggaccgt ctccgcatcg ctgaatatgg tgatcctgat
    32161 ggtgatgtcg gtctcggtcg aaatcggccg ctggctgtgg tttaacggcg gtcgcttgcc
    32221 gtttcatctg ttagccgtcg tggcgggcgt tatcgtcgtt ttcaccctgg caggattgct
    32281 taatcgcgtg cgccaacatc aggctgccga gctggcggag gagtggtgat ttttgcgcga
    32341 tcctgccgtc aggctctatt cttaacgtta tgaataaact cattgaactc agacgcgcca
    32401 aaatgttggc gctctcttta ctgcttatcg ccgctgctac ctttgtcgtt acgctgtttt
    32461 tgccgcccaa tttttgggtg agcggcgtga aggcgattgc tgaagcggcg atggtcggcg
    32521 cgctggcgga ctggtttgcg gtggtggcgc tgtttcgccg cgtgccgatt ccgatcattt
    32581 ctcgtcatac ggcgattatc ccgcgtaata aagaccggat tggcgaaaat ctcggccagt
    32641 tcgtgcagga aaaatttctc gatacccagt cgctggtggc attgattcga cgccacgaac
    32701 cggtgttgtt gattggcaac tggtttagtc agccagaaaa tgcccgccgc gttggtcagc
    32761 atctgttgca gatcatgagc ggttttcttg aactgaccga tgatgcgcgt attcagcgcc
    32821 tgcttaagcg cgcggtccat cgggcgattg ataaggtcga tctttccggc accagtgcgt
    32881 tgatgctgga gagtatgacc aaaaacgatc gtcatcaggt gctactggat acgctgatcg
    32941 cacagttgat cgcccttctc cagcgcgata aatcgcgcaa gtttattgcc cagcagattg
    33001 ttcgctggct ggagagtgag catccactga aagccaaaat tctccccacc gaatggttgg
    33061 gcgaacatag cgcggagttg gtttctgacg cggtgaattc tttgcttgat gatatcagcc
    33121 gtgatcgtgc gcatcagatc cgtcatgcgt ttgatcgcgc cacttttgcc ctgatcgaca
    33181 agttgaaaaa cgatccggaa atggcagcgc gagccgatgc cgtaaaaagt tatctgaaag
    33241 aagatgaagc ttttaaccgc tatctcagtg a
//
LOCUS       DAFATA010000043        33084 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFATA010000043 DAFATA010000000
VERSION     DAFATA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.044"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..552
                     /locus_tag="LGJ20_002448"
     CDS             <1..552
                     /locus_tag="LGJ20_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBK2386499.1"
                     /translation="ASQTRDKDALTREGVAKVLDDLKAMDFEFIVCDSPAGIETGALM
                     ALYFADEAIITTNPEVSSVRDSDRILGILASKSRRAENGEEPIKEHLLLTRYNPGRVS
                     RGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYADTVERLL
                     GEERPFRFIEEEKKGFLKRLFGG"
     gene            556..822
                     /gene="minE"
                     /locus_tag="LGJ20_002449"
     CDS             556..822
                     /gene="minE"
                     /locus_tag="LGJ20_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBK2386500.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1194..1538)
                     /locus_tag="LGJ20_002450"
                     /pseudo
     CDS             complement(1194..1538)
                     /locus_tag="LGJ20_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1573..1692)
                     /locus_tag="LGJ20_002451"
     CDS             complement(1573..1692)
                     /locus_tag="LGJ20_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK2386501.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1653..1838)
                     /locus_tag="LGJ20_002452"
     CDS             complement(1653..1838)
                     /locus_tag="LGJ20_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386502.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            1939..2112
                     /locus_tag="LGJ20_002453"
     CDS             1939..2112
                     /locus_tag="LGJ20_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386503.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            2114..2458
                     /locus_tag="LGJ20_002454"
     CDS             2114..2458
                     /locus_tag="LGJ20_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBK2386504.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            2462..2797
                     /locus_tag="LGJ20_002455"
     CDS             2462..2797
                     /locus_tag="LGJ20_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBK2386505.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(2854..5175)
                     /locus_tag="LGJ20_002456"
     CDS             complement(2854..5175)
                     /locus_tag="LGJ20_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2386506.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVNITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGAGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGSHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(5292..5501)
                     /locus_tag="LGJ20_002457"
     CDS             complement(5292..5501)
                     /locus_tag="LGJ20_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386507.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(5901..6119)
                     /locus_tag="LGJ20_002458"
     CDS             complement(5901..6119)
                     /locus_tag="LGJ20_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386508.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(6251..7774)
                     /locus_tag="LGJ20_002459"
     CDS             complement(6251..7774)
                     /locus_tag="LGJ20_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2386509.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(8106..8354)
                     /locus_tag="LGJ20_002460"
     CDS             complement(8106..8354)
                     /locus_tag="LGJ20_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386510.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(8467..8733)
                     /gene="ariR"
                     /locus_tag="LGJ20_002461"
     CDS             complement(8467..8733)
                     /gene="ariR"
                     /locus_tag="LGJ20_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HBK2386511.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(8762..9034)
                     /locus_tag="LGJ20_002462"
     CDS             complement(8762..9034)
                     /locus_tag="LGJ20_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HBK2386512.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(9077..9313)
                     /locus_tag="LGJ20_002463"
     CDS             complement(9077..9313)
                     /locus_tag="LGJ20_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HBK2386513.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            9627..10838
                     /gene="bluF"
                     /locus_tag="LGJ20_002464"
     CDS             9627..10838
                     /gene="bluF"
                     /locus_tag="LGJ20_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBK2386514.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            11043..11774
                     /gene="bluR"
                     /locus_tag="LGJ20_002465"
     CDS             11043..11774
                     /gene="bluR"
                     /locus_tag="LGJ20_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBK2386515.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            11995..12399
                     /locus_tag="LGJ20_002466"
     CDS             11995..12399
                     /locus_tag="LGJ20_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBK2386516.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            12452..12562
                     /locus_tag="LGJ20_002467"
     CDS             12452..12562
                     /locus_tag="LGJ20_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386517.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            12798..12842
                     /locus_tag="LGJ20_002468"
     CDS             12798..12842
                     /locus_tag="LGJ20_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386518.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            13095..13418
                     /gene="iraM"
                     /locus_tag="LGJ20_002469"
     CDS             13095..13418
                     /gene="iraM"
                     /locus_tag="LGJ20_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBK2386519.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(13521..14771)
                     /gene="icd"
                     /locus_tag="LGJ20_002470"
     CDS             complement(13521..14771)
                     /gene="icd"
                     /locus_tag="LGJ20_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBK2386520.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            14943..15596
                     /gene="rluE"
                     /locus_tag="LGJ20_002471"
     CDS             14943..15596
                     /gene="rluE"
                     /locus_tag="LGJ20_002471"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBK2386521.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            15606..16067
                     /gene="nudJ"
                     /locus_tag="LGJ20_002472"
     CDS             15606..16067
                     /gene="nudJ"
                     /locus_tag="LGJ20_002472"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBK2386522.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            16121..17227
                     /gene="mnmA"
                     /locus_tag="LGJ20_002473"
     CDS             16121..17227
                     /gene="mnmA"
                     /locus_tag="LGJ20_002473"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK2386523.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            17263..17904
                     /gene="hflD"
                     /locus_tag="LGJ20_002474"
     CDS             17263..17904
                     /gene="hflD"
                     /locus_tag="LGJ20_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBK2386524.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            17908..19278
                     /gene="purB"
                     /locus_tag="LGJ20_002475"
     CDS             17908..19278
                     /gene="purB"
                     /locus_tag="LGJ20_002475"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK2386525.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWLQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            19446..20117
                     /gene="phoP"
                     /locus_tag="LGJ20_002476"
     CDS             19446..20117
                     /gene="phoP"
                     /locus_tag="LGJ20_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBK2386526.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            20117..21577
                     /gene="phoQ"
                     /locus_tag="LGJ20_002477"
     CDS             20117..21577
                     /gene="phoQ"
                     /locus_tag="LGJ20_002477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBK2386527.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            21653..22774
                     /gene="roxA"
                     /locus_tag="LGJ20_002478"
                     /gene_synonym="ycfD"
     CDS             21653..22774
                     /gene="roxA"
                     /locus_tag="LGJ20_002478"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBK2386528.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(22920..24149)
                     /gene="pepT"
                     /locus_tag="LGJ20_002479"
     CDS             complement(22920..24149)
                     /gene="pepT"
                     /locus_tag="LGJ20_002479"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK2386529.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            24399..25535
                     /gene="potA"
                     /locus_tag="LGJ20_002480"
     CDS             24399..25535
                     /gene="potA"
                     /locus_tag="LGJ20_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBK2386530.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            25519..26376
                     /gene="potB"
                     /locus_tag="LGJ20_002481"
     CDS             25519..26376
                     /gene="potB"
                     /locus_tag="LGJ20_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBK2386531.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            26373..27167
                     /gene="potC"
                     /locus_tag="LGJ20_002482"
     CDS             26373..27167
                     /gene="potC"
                     /locus_tag="LGJ20_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBK2386532.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27164..28210
                     /gene="potD"
                     /locus_tag="LGJ20_002483"
     CDS             27164..28210
                     /gene="potD"
                     /locus_tag="LGJ20_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBK2386533.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            28268..28729
                     /locus_tag="LGJ20_002484"
     CDS             28268..28729
                     /locus_tag="LGJ20_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HBK2386534.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            28726..29064
                     /locus_tag="LGJ20_002485"
     CDS             28726..29064
                     /locus_tag="LGJ20_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386535.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNG"
     gene            29167..29514
                     /locus_tag="LGJ20_002486"
     CDS             29167..29514
                     /locus_tag="LGJ20_002486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386536.1"
                     /translation="MAVLQAQILKQPTPPLTYYPHPGQQHENAPPGSTNTLGFIAWAA
                     TFILFSWIFCYTTRFIYALKFAVAMTIANMGYQALCLYIDNSFAITRISPLWAGLIGV
                     CTFIAALLLTSKR"
     gene            complement(29653..30474)
                     /gene="cobB"
                     /locus_tag="LGJ20_002487"
     CDS             complement(29653..30474)
                     /gene="cobB"
                     /locus_tag="LGJ20_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK2386537.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(30490..31401)
                     /gene="nagK"
                     /locus_tag="LGJ20_002488"
     CDS             complement(30490..31401)
                     /gene="nagK"
                     /locus_tag="LGJ20_002488"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBK2386538.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31430..32674)
                     /gene="lolE"
                     /locus_tag="LGJ20_002489"
     CDS             complement(31430..32674)
                     /gene="lolE"
                     /locus_tag="LGJ20_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBK2386539.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32674..>33084)
                     /locus_tag="LGJ20_002490"
     CDS             complement(32674..>33084)
                     /locus_tag="LGJ20_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK2386540.1"
                     /translation="LLPDFTALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANH
                     RPSELSGGERQRVAIARALVNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAF
                     LVVTHDLQLAKRMSRQLEMRDGRLTAELSLMGAE"
BASE COUNT         8780 a         8021 c         7625 g         8658 t
ORIGIN      
        1 gcatcgcaaa cacgcgataa agatgccctc acccgtgaag gggtcgccaa agttcttgat
       61 gatctgaaag cgatggattt tgaatttatc gtttgtgact ccccggcagg gattgaaacc
      121 ggtgcgttaa tggcactcta ttttgcagac gaagccatta ttaccaccaa cccggaagtc
      181 tcctcagtac gcgactctga ccgtatttta ggcattctgg cgtcgaaatc acgccgcgca
      241 gaaaatggcg aagagcctat taaagagcac ctgctgttaa cgcgctataa cccaggccgc
      301 gtaagcagag gtgacatgct gagcatggaa gatgtgctgg agatcctgcg catcaaactc
      361 gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct ctaaccaggg tgaaccggtc
      421 attctcgaca ttaacgccga tgcgggtaaa gcctacgcag ataccgtaga acgtctgttg
      481 ggagaagaac gtcctttccg cttcattgaa gaagagaaga aaggcttcct caaacgcttg
      541 ttcggaggat aagttatggc attactcgat ttctttctct cgcggaagaa aaacacagcc
      601 aacattgcaa aagaacggct gcagattatt gttgctgaac gccgtcgcag cgatgcagaa
      661 ccgcattatc tgccgcagtt gcgtaaagat attcttgagg tcatttgtaa atacgtacaa
      721 attgatcctg agatggtaac cgtacagctt gagcaaaaag atggcgatat ttctattctt
      781 gagctgaacg tgaccttacc ggaagcagaa gagctgaaat aagcccgctg taaaaacgca
      841 tttatcttca aggcagagtc atttctgcct tgagtttttc atccctctca tccacgttgt
      901 ggtaaagcgg cgagtattct tgctgatact cctcattgct atttccacct ccccctttac
      961 accttaaggc tgttcccacg cacagaacaa aatgtttatg cctggttgag taaataacct
     1021 cattgttagt gtggatacac tgggggatga taaccccgca atttcattat tcattgttct
     1081 cccataggat gaggtttccc gccgtaactg gcgggctttt tttgccccag aattttccct
     1141 tttagccccc tgtaaacgaa aactgcgccg aagcgcagtt taatcgttga tggtcagaac
     1201 gaccagttca caccagccac cgcgttccac ggggactcca caccggcacc atggctatac
     1261 cccaccccga aatgcccgct taacgtactg ctgaatgagg ctttaatacc tgcctggtat
     1321 attccacgtc tgcccgacaa atcattgaca aaattaccgt cactattcac tttcacccga
     1381 ttatcatcga caaattcttt gcgcacagcc gccttcaggc agggttcaat ttccataccg
     1441 ttccccagac gcatgttgta actcagcgtt gcacccagtt cacgatatat actgcgggta
     1501 tcgactgatt tcgattccat gccattggat aaatgatagt agcgtctaag tgacctaagc
     1561 tctgctgcaa catcagcccg caggcaccag accaggggat tcatcctgaa gagacagcgc
     1621 aggtgtattg tgttcaccgc tcaccaaaga catcatgatg aaatgatgat atcccgcata
     1681 agaaagaggc attttttaca cgcagtgcgc tgaagtgtag ttggataaaa aatcactctt
     1741 ttcagataat actgtcgtac gcttatcttt cgacaatgag gccgtcggca aaataaaatg
     1801 actaatgtaa tcgttgctga tgaatatctt ctgctcacat aaaaatcaca caataatatt
     1861 gataaatcag attatcttac ctttacaaca ttgcgttaac agtcaatgac acttcttgaa
     1921 taaaaatatt gtcaatcaat gagttattcc tcatttaaga ttattctcat tcacgtaaaa
     1981 aacataattc ctatcatcac agccacgctc attttgaatt atctgaataa tagcgagcgt
     2041 tctttagtta agcagatctt aattgaagac gaaattattg tgtgtgcaac ttacttaata
     2101 ccggacatct aagttgaaga aaaaaatact tgccttcggg cttatctcag cattgttctg
     2161 ctccacgcca gcgatggcgg atatgaaccg caccacgaaa ggtgcattgc taggcgcagg
     2221 cgttggttta cttactggca atggtgttaa tggcgtactg aaaggtgcag ctgttggcgc
     2281 tggtgttggt gcagtaacag aaaaaggccg cgacggtaaa aatgcgcgta aaggtgcaaa
     2341 agtgggtgcc gccgttggtg ctgtcactgg cgttctgacc ggcaatggcc tcgaaggggc
     2401 gattaaaggt gccgtgattg gcggtacggg tggtgcaatt ctgggtaaaa tgaaataagg
     2461 aatgatgatg aaaaaatttg ctttactggc gggcctgttt gttttcgcac caatgacctg
     2521 ggcgcaggac tacaatatta aaaacggttt gccgtcagaa acctatatta cttgcgcaga
     2581 agccaacgaa atggcaaaaa ctgacagcgc acaggttgca gaaattgttg cggtaatggg
     2641 taatgccagc gttgccagcc gtgatttaaa aattgagcaa tctccggaac tgagcgccaa
     2701 agtggtagaa aaactgaatc aggtttgcgc taaagacccg caaatgctgt tgatcacggc
     2761 aattgatgac accatgcgtg ctatcggcaa aaaataatat ttcatatgta ccgcgtcgat
     2821 gaatacgacg cggtttttta gctaacggcg ggatcagaac ttatatttca ggcccaccat
     2881 cactgcggtg tcattgtagc cattatcacc cagctgcacg cccacattcc cccagacgct
     2941 ggccgctgga ttcaggttgc catttacgcc aagtttaatt tcccccaaat tactcgcccc
     3001 atcttgttcg actttcacac cgttcattga gacagcaaaa tctttactgt tatggatcca
     3061 gttcatttca acaaaaggtt caaagtcgtc accgtgcgaa ccgctgtttt tctcctgagt
     3121 acgaataaag gtgcggaaac caagtcgggt ctggatgttg ttatttgcgt cattctcaat
     3181 acgtgagcca ttggcttcgg tgtgcgcatt atgatcgacg cccatccaga tagcctgtgc
     3241 ctgtggctgg aatatccacg tgtatttcgc cgcttccaga ccaaaggatt tattcagggc
     3301 aaagccgtaa cccgcttcca gcgagacggt tgcgcctttc gcactatagg attcataggc
     3361 taattcatcc cctttcaccg agtttttaaa ccagctatat tgcgcccagg cgtcaatgta
     3421 tgcgcctttt ttactgatgt catcggcaaa ccaggtggca tacagccctg cgcaatagcc
     3481 tctgacactc cctttcgaac gataatccga cacgctggaa tgagttgagt tgtagtcgcg
     3541 ggcataacca gccatcactc ccaaacgcca actgtcactg tcggtaaaac caccggttaa
     3601 cagatcggcc cctaactgcg agacgtagcg atgcgaggtc gttctcaact gtccgttgct
     3661 gtcacgccag gcattatgcc cgccaatttg acgtagccaa agtcggcttg aacgctcctg
     3721 ttcagttaca ggatcgatgt agcgcatttc acccgcacgg tcgttcagac gcataacaaa
     3781 aagagtgtta gcggctgcca ggttggcgac gtaacttcca gcttcgaggc gcaggacggg
     3841 ggtaggcgca acagtaggcg ctgaacttcc tccggagttt gtttcaccgc cgctcgttgc
     3901 ctggaagtta gtcagatacc agtttttatt ggtcgcttcg gtattacctc gggttaatga
     3961 atattcgtag gcaccggcga aaatcctcga atccttaata aattccccat ttgattcccc
     4021 ctcaacgctg ataatctcta tcccatttaa cgtgtaagct ccgctgcctc ctgcattggt
     4081 aactttaact cgggttgttc cagaggtatt tccattaata acgactttat ccgtagctga
     4141 attatcatca cccaattcag ttctgagatg caatacgcca ttattaccaa cgtaattttc
     4201 agttattgtt aatcttgttg gttcgacatc ccgtccatct gcccgggaaa tatagaccgt
     4261 actattatca accgtgaggt tgctcaccgt tgagtcagct ttaatatccc aggtactatt
     4321 atctgaaagc gacagatagg tatgagtgtt atcatctgtt gaaatattag ctgaacccgt
     4381 tatttttgaa ttagttgcat cgacggtcaa atgaccttta atattattga taaaaacatc
     4441 tgcagccgat gatacctcac tatccgtaat ggtcacgtta ctcgtctcat ttttatggta
     4501 gaggatttca tttgctgacc gtagtgagct accagataaa atatctaccg ttgcttcctg
     4561 accatttatg gaagactcca gcacaattcc agccccctga tgcgcgactg cagatgtatt
     4621 tttcagcgtc accgtgccgc cattataaat tttagcaatc gcggcgtcag gacccgttgt
     4681 ataaatttct ccctggttaa atgaaactgt cccctggcgg gcagcgatgg catgagcatt
     4741 gtccccatgc gtggtgatat taacatcggt taatgtaacg ctggcattgg tataatcaag
     4801 cgtgtcacca gccttgacga cagtgccatt agcgtacgcg gcataacttt ctgttccgtg
     4861 ggttgtgatc tccccttttg ccatatccag ttttgctatg gcattatttt cattacttcc
     4921 tgtatcggta gtactttctt catcaagaga agatacacac gcacgcgcgc ttccgggaga
     4981 tgcacacgca agaacgccga ctgcgtgatt acccgcagta acaataaatg tatcatctcc
     5041 gtcagaaaaa gagggggtaa ccgaaccgtc agagttgact cttattatgg tgtcagtatt
     5101 caggtttcca ctcgtttgac ttagcaaacc aattgagtaa tcccccgttg tgacaatcgt
     5161 gtttttaccc tgcaaaataa cagcaccgcc aaattcactt acgattgcgc tagctgttaa
     5221 tgctgtgctt agaggactat tgtcagaagg atataaaccg cccgttgtta caatgacgcc
     5281 attgttaatg gttaacttgc taccctctcc tgcaacggat acccgtaact tccattaccc
     5341 gttgttataa ctagcgcatt atcaatatct tttacttttc catcttttat tgccacagca
     5401 tgtttatatt catcgtgctg aataaactgt tcagcatatg cattacccac cgccagagca
     5461 ataagtccca aactaaaccc ggagagtttt ttcagtttca tagattaacc atccattcat
     5521 tggattaaca aacattcctg gtcaaataac ctatgcgttc agattaccaa aacggagggt
     5581 aaagacaaaa acaaaagcac aaaaaattta aatagataaa cagcgacaat atataaatga
     5641 taaataagtc ggcattatca tttttcacct tattttcatg acattgatca ctttagggtg
     5701 acgattttgt ttaacaaata actcttcgac atcaaaattg ttaatagttg cgatgaggat
     5761 tatttcggtg atatataaaa acaaccgtga taacaccagg gttgacaact atttgcgcaa
     5821 taatgccttt ccttcaatga tccaggcgtt cactgccagc aacccattca gccgcatact
     5881 gacagtgaga aactgaaaga ttattgctga tccctgtggt tgacgagtct ttctcttaat
     5941 cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc
     6001 acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt gatcagcata
     6061 aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact attgttcatg
     6121 ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa
     6181 tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac
     6241 tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata attttgacca
     6301 ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt
     6361 ggttcagata gtcaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa
     6421 gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttta
     6481 cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca tgaagataag
     6541 aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca
     6601 gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt
     6661 ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga
     6721 caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg
     6781 gcaatttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgcgtaa
     6841 gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt gatatatatc
     6901 caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac
     6961 catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga
     7021 tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca gcggcgcagg
     7081 cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa
     7141 agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg
     7201 tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata atgtcatccg
     7261 ccacgcgtaa tacatacctt acccctttca agggtacatt aagtgcccca cgaatatgct
     7321 gatcgcttat cgtaaccaaa atgcgcgtat ctgcaaattg gttctgatag agcaggatag
     7381 gtaatctatt aacggtgtcg attgctggag ccaacagtaa attactgtcc gggaaaacag
     7441 gaatacgaga caacaggacc cgattcccag gcagggatgt acaccagact attccctgtt
     7501 tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca agctgatatt
     7561 gctcctcgag gttgcattcc ttcccggcaa tgttaaccgc tgtttgagtc gcttgtgacg
     7621 cttcttcaag tatatgattg atattgttgg cggcatatct cgctcccgcc aggtactctg
     7681 cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata aacaagcata
     7741 tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca aagggtttaa
     7801 tacatgaaaa tgccattcat tcgctcgcgc tggcatctac agagagcatg gtagagagca
     7861 taagaaatat ccacgccgaa gataaaattt tctccatctt gatctaaccc attaattatt
     7921 gagcataatg taggcagtac aaaataagtt aggcgggata tcaggcgtca agaatggaga
     7981 gcgagaactc tccattcttg acacctgata ttgcggacat aataagaaag cataacgcct
     8041 gaaatgctca ctttgcatca gcatggtgat acagctgatg tttattctaa aaccttactc
     8101 aagttctaag agagcacgga ttccctgtca ttaataatga tatccgttag tgtagtctta
     8161 tgcatttcaa tgtactgcaa tatttgcaga tagagatcag aaagaatcac actggtttca
     8221 tcttttaaac gcaagcgtaa agcctgatca ataatatgac tatcaacata cccatgcgtc
     8281 atgaacacct ccctctcgag atttttaaac aaagcacatt gaaaaataaa tctctctggg
     8341 attgaattat tcatgtcagc ctcgtagaaa tattaaccca ataaaattat gctgtgtata
     8401 gtctaaagcg acagacaaaa ataagatgaa tgagttttct ataaacttat acttaataat
     8461 tagaagttac atatcatcag ctgtgtatcg caacacgatt tccagtgttt ttctaatcac
     8521 atcagctttg agaatatcac ttgttgtttc cagggagtgt atcagactta agataatatt
     8581 tttattatta acattatcgc ctgaaagcat taaattggtg acagcctgcc ctaacactgc
     8641 tgactcttct tctaacaaat tacccgaact gcgaaagtaa cttgctaacg ctttatcagt
     8701 tattgcatta tgaattgtag tatcttcaag catggtaacc tctcatctta cttatgaaat
     8761 tttaatgtat tctgtttatt ttcttaccat tgaacatact tttattgtca gctgtcgctg
     8821 ttcttcggtt tggccctcct gaatcactat tgctaaaatc acgaattatg ctgtcaatta
     8881 aatcgcgtcg gaaagcatct tcctcagccg atttcattac gagtaattgc ttaataagag
     8941 tctgcaatga aaaatttatc ttcatgcgca gtatctggat caccgcctgg ccagtaattt
     9001 catattttat acgttcatta tcagatgtct tcataacgac ttcatagcta acctgttaaa
     9061 taatcaggct gttgcattat tcaaaaagca acccaattag tgcattataa tgtttctgtt
     9121 cctcttcccc agtcgcgtgt tccagtcggc aaagaagttt ggcacaaagc gactttcgac
     9181 tcagattacg tccatctttc aaaatctcag tcacaatttc cccgagagtt tcctgctgag
     9241 taggcaagtt agcttttgca aagtaacgag tgattgctcc cgcagaatct aaagtcactg
     9301 aattttgatg catgctacgc ctctgttaaa aatgttaatc aatgttaagt gaggtaacaa
     9361 tagcaaactt gtacgaaata tgtgtacaga atgataaatt aattttttgc aattagtgct
     9421 aatgcatata aaacaatgtg ttatgataaa acatattgca tcatttaact gacaatctat
     9481 acaagatttt tcgccaacta gtgttcaact ttttccattt tttatcaatg taaagaaacg
     9541 gttatgtttt ggtattaaaa gaaagacaca agaagatatg tctgttaccg tcttactctc
     9601 gcctcaccca ttaccctgga ttgttaatgc ttaccaccct tatttatcgt agccatatac
     9661 gtgacgacga acctgtcaaa aaaatcgaag aaatggtttc gatagcaaat cgcaggaaca
     9721 tgcagtctga cgtaacaggg atcttactgt ttaatggttc tcattttttc cagcttctgg
     9781 aaggtccgga agaacaggtt aaaatgatat atcgggctat atgccaggat ccacggcact
     9841 ataatattgt tgagctgctg tgcgattacg cgcctgctcg ccgttttggc aaagcgggaa
     9901 tggaattatt tgatttgcgc ctgcacgagc gagatgacgt tttacaggcc gtattcgaca
     9961 aaggcacatc aaaatttcag ctaacttatg atgacagagc gctacaattt tttcgtactt
    10021 ttgtccttgc aaccgaacaa tcaacctatt tcgagatccc tgccgaagac tcctggcttt
    10081 ttatcgctga cggatctgat aaagaacttg attcctgtgc cctttcacca actataaacg
    10141 accactttgc ctttcatcct attgtcgatc ccttatcgcg gcggataatc gcttttgaag
    10201 ccattgtgca aaaaaatgaa gatagcccat cagccatagc ggttgggcag cgtaaagacg
    10261 gggaaatcta cacagcggat ctcaaaagta aggcgcttgc attcacgatg gcacacgcac
    10321 ttgagctcgg tgataaaatg atttcaatca atctattacc tatgaccctg gttaacgaac
    10381 ctgacgcagt ctctttttta cttaatgaaa taaaggccaa tgctctggtg cctgaacaaa
    10441 tcatcgttga atttactgaa agtgaagtca tatctcggtt tgatgagttt gccgaagcga
    10501 ttaaatcgct aaaggctgct ggtatcagtg tagcaattga tcattttggc gcaggttttg
    10561 ctggtttgtt actcctgtca cgcttccagc ctgacagaat taaaatcagt caggaattga
    10621 ttaccaatgt tcataaaagt gggccacggc aggcaattat tcaggcgatc ataaaatgct
    10681 gtacatcact tgaaattcaa gtcagtgcta tgggcgtggc aacaccagaa gagtggatgt
    10741 ggcttgaatc cgcaggaatt gagatgtttc agggagatct gtttgctaaa gctaaattga
    10801 atggtatccc ttccatagcg tggccagaga aaaaataatt ttcagcacat tctttcacat
    10861 gatttcagta aatcatcgtg aaaatgaata cgttacgttt aaatttaatc cacattaaaa
    10921 ataatgtttc atccaattta gctgaaatct tatttatact ttgtacaatt ttagcgtata
    10981 ttgtacaaag tattattggg tcgtgtacag gcgacggaga tttgtgaccg caaggaggaa
    11041 ttgtggctta ttacagcatt ggtgatgttg ctgaacgttg cgggattaat cctgtcactc
    11101 tccgggcctg gcaacgccgc tacggtttgt taaaaccaca gcgcagtgaa ggcggacacc
    11161 gactctttga tgaagaagac atacaacgca tcgaagagat caagcgttgg ataagtaatg
    11221 gcgtccctgt aggcaaagtt aaagcattac tggaaaccac cagccaggat acggaagatg
    11281 actggagccg cctgcaagaa gagatgatgt caattcttcg catggctaat cctgccaaac
    11341 tacgcgcgag aattatttca ctgggtcgag agtacccagt agatcaattg attaatcatg
    11401 tttaccttcc tgttcgccag cgtctcgtgc ttgatcacaa cacctcccgc attatgagca
    11461 gtatgtttga cggcgcatta attgaatacg cagcaacctc gcttttcgaa atgcgccgta
    11521 agcccggtaa agaagccatt ctgatggcgt ggaatgttga agagagagca cgattgtggc
    11581 tggaagcatg gcgtttatca ttgtcaggat ggcacatttc tgttcttgct gatcccattg
    11641 aatcgccgcg cccggaactg ttcccgacgc aaacattgat tgtctggaca ggcatggcac
    11701 caacgagaag gcaaaacgaa cttttacaac attggggtga gcaaggttat aaagtcatct
    11761 ttcatgcccc ctaatttcac ctttatcccg ccccttagac agaatgttat aatctgaacc
    11821 cacaacagcg tttgtaccgg agctggtccg ttgcaaaatg ctcaccggga gaattcagat
    11881 attccctcta tcacactctg tacatatcac agccaaagga cagaccagat tgaagtggta
    11941 aaaccgatcg tgcacactac gcagtctcac aactaacaag aggttattat cctgatggat
    12001 caggtcgttg tttttcaaaa gatgtttgag caagtgagaa aagagcaaaa cttctcctgg
    12061 ttttattcag aattaaaaca tcaccgtatt gcacattaca tttattatct ggctacggat
    12121 aacatcagaa ttattactca cgatgacacg gttttgttat taagaggaac caggaacctg
    12181 ttaaaagtta gtacgaccaa gaaccctgct aaaataaaag aggccgcatt gcttcatatt
    12241 tgcggaaaat ctacatttcg ggaatactgt tcaacactag caggcgcggg cgttttccgg
    12301 tgggttactg atgttaatca taacaaacgc agttactacg ccattgataa tacgctttta
    12361 tatattgaag atgtagaaaa taataaacca ttaatctagc taaagttgga tgcttaagaa
    12421 atgcttcata attcagtaag gcattagcat aatgggaata aaagtacaga gactatccct
    12481 atggatgata aatactgtcc ttttattgct acccataaat aatcaccatg ccaatactat
    12541 caaattgata tttgaaatgt gatcacttga ctttctatac gttattttat aacggttaat
    12601 atatttataa aaacaacggg tgtgccatac gcccgtttca atacttaacg cacatgtgtt
    12661 ttggtttagt catcatccag ttatatgtat tttagccagg aacaggttaa acctttccta
    12721 tataactcaa aaattgaaac cttattctca tgtcatgctt atattcatta ttatcgttat
    12781 ataaaaaggc aaccataatg tttagcaaat tggcacaaag tagcataaag gctatgtttt
    12841 aattacagga tgttcagtca tttgaatgta taacattata gctaaacaaa tctaaaacga
    12901 agtgaataat ttattgtttt cactaaatct cattttgttt aacatccatt gagattcctt
    12961 gctttaaatt ttgttttata taagcaatcg ttttaattaa tttatttttt gaaggggtaa
    13021 tatactcata tgcaaaataa agaaatgaac atccaaatga actatattaa ataccgtggg
    13081 ataagacata acaaatgaag tggatagtaa ttgacacgat aattcaacct tcatgcggca
    13141 tatctttttc agccatatgg ggtaatatga aaatgatcat ctggtatcaa tctactatat
    13201 ttctccctcc tggcagtata tttacaccgg ttaaatctgg aattatcctt aaagataaag
    13261 aatatcctat tactatttat aacatcgcac cattcaacaa ggatttatgg agtctactca
    13321 aaagcagtca agagtgtcct ccaggagaaa gcaaaataac aaataaatgt ttgcataata
    13381 gttgcattat aaaaatatgc ccatatgggc tcaagtaaag ggttttatat aaatatgctc
    13441 gatatgctat taataatacc tacataaata aaacaacggg cgtgttatac gcccgttgca
    13501 atattaaaca catgtagtga ttacatgttc ttgatgatcg catcaccaaa ttctgaacat
    13561 ttcagcagtt tagcgccttc catcagacgc tcgaagtcat aggttacggt cttcgcattg
    13621 attgcgcctt ccataccttt aacaatcagg tctgcggctt cagtccagcc catatggcgt
    13681 aacatcatct cagcggagag aataatagag ccagggttta ctttgtcctg accggcgtat
    13741 ttcggcgcag taccgtgggt ggcttcaaac agggcgcatt cgtcaccgat gtttgctcca
    13801 ggggcgatac cgataccgcc aacctgcgct gccagagcgt cagaaatgta gtcaccattc
    13861 aggttcatac aggcgataac atcatattca gccggacgca gcaggatctg ttgcaggaat
    13921 gcatcagcaa tcacgtcttt aatgacgatc tctttgccgg tgttcgggtt tttaactttc
    13981 agccacgggc cgccgtcgat cagttcaccg ccaaactctt cacgcgccag ctggtagccc
    14041 caatctttaa acgcgccttc ggtgaacttc atgatgttgc ctttgtgcac cagagtcaca
    14101 gagtcacgat cgttagcaat tgcgtattcg atcgctgcac gaaccagacg tttggtgcct
    14161 tcttccgaac acggcttaat accgatacca caatgttccg ggaagcgaat tttcttcacc
    14221 cccatctctt cacgcaggaa tttaatcact ttctcggcgt cggcagagtc tgctttccat
    14281 tcgatacccg cataaatgtc ttccgagttt tcacggaaga taaccatatc ggtcagttca
    14341 gggtgtttaa ccgggcttgg agtgccttga tagtaacgta ccggacgcag gcagatgtag
    14401 agatccagtt cctggcgcag ggcaacgttc agagagcgaa taccgccacc aacaggagtg
    14461 gtcagcggac ctttaatggc aacgcgatat tcacgaatca gatcaagagt ttcagcaggc
    14521 agccagacgt cctgaccata aacctgtgtg gatttttcac cggtgtaaat ttccatccag
    14581 gagattttac gctcgccttt ataggctttc tcgactgcag cgtcgaccac tttcagcatg
    14641 gctggggtta catctacacc gattccatca ccttcaatgt aagggataat cggattttca
    14701 ggaacgttga gtttgccgtt ttgcagggtg atcttcttgc cttgtgccgg aacaactact
    14761 ttactttcca ttcacctctc cttcgagcgc tactggtttg ctcgtctgcc accacgttgc
    14821 atatgcgttt gcgtcctgcg atacggatgc tttagagcaa ttttttgtta atgatttgta
    14881 attggcttgt caatactacc ctattgtttg ccgtcatgaa agatgcgcgt tattaggcta
    14941 taatgcggca attcataatc tctgaaaata ccatgcaaaa aacttctttt agaaatcacc
    15001 aggttaagcg attcagctcg caacgttcta ccaggcgtaa acctgaaaac cagcccacgc
    15061 gtgtgatcct gttcaataaa ccctacgatg ttcttccgca gttcaccgat gaagccggac
    15121 gcaaaacatt aaaagaattc atcccggttc agggcgttta tgcagcaggt cgtcttgacc
    15181 gcgatagcga agggttactg gtgctgacca ataacggcgc gttgcaggcg cgtttaaccc
    15241 agccgggcaa acgcaccggg aaaatctatt atgtgcaggt ggaaggtatt cccacacaag
    15301 acgcacttga agccttgcgc aatggcgtaa ccttaaatga tggccctacc ctgcccgcag
    15361 gcgcggagct ggttgacgaa cccgcgtggt tatggccgcg gaatccacca attcgtgaac
    15421 gcaaaagtat tcccaccagc tggctgaaga tcaccttata tgaaggacgt aatcgccagg
    15481 tgcgccgcat gaccgcccat gttggtttcc ccacgctgcg actgattcgc tatgcgatgg
    15541 gagattactc tttggataat cttgccaatg gcgaatggcg agaagtgaca gattaaggat
    15601 gcaaaatgtt taaaccgcac gttaccgttg cttgcgtggt gcacgcagaa ggcaaatttt
    15661 tagtcgttga agagacgatt aatggtaaag cgttatggaa ccaacctgcc gggcatctgg
    15721 aagccgatga aaccttagtg gaagccgccg cccgtgaact gtgggaagaa accggcatca
    15781 gcgcgcagcc gcaacacttt attcgtatgc atcagtggat tgcgccagat aaaacgccgt
    15841 ttttgcgttt cctctttgcc attgagcttg agcaaatatg cccgacccag cctcatgaca
    15901 gcgatatcga ctgctgtcgt tgggtcagcg ccgaagaaat tttacaggcg tcaaatcttc
    15961 gttcgccgct ggtggcggaa agtatccgtt gttatcaaag cgggcaacgc tatccgctgg
    16021 agatgattgg cgattttaac tggcctttta caaagggtgt catctaaaag gctgcgtgat
    16081 agaatacgcc gccttgaagt tcaatgtcgt gagtgatcca atgtctgaaa ccgcaaaaaa
    16141 agtaatcgtc ggcatgtccg gcggtgtcga ttcctccgtt tctgcctggc tgttgcaaca
    16201 acagggatat caggtcgaag gcctgtttat gaagaactgg gaagaagacg acggtgagga
    16261 atattgcaca gcggcagcgg atctggctga tgcccaggct gtctgcgaca agctcggcat
    16321 tgaactgcac accgttaact ttgctgccga gtactgggac aacgtcttcg aactgttcct
    16381 tgccgaatat aaagccggtc gcacgccgaa tccggatatt ctgtgcaaca aagagatcaa
    16441 atttaaagcc ttcctcgaat ttgccgccga agatttaggt gccgattata tcgctaccgg
    16501 tcattacgta cgtcgggccg atgtcgatgg caagagccgc ctgctgcgtg gtctggacag
    16561 caataaagac cagagctact tcctttatac gctcagccat gagcagattg cgcaaagcct
    16621 gttcccggtc ggcgaactgg aaaaaccgca ggtgcgtaag attgctgaag atcttggtct
    16681 ggtcaccgcg aagaaaaaag actctaccgg catttgcttc attggcgaac gtaaattccg
    16741 cgagttcctg ggccgttatc tcccggcgca accgggcaaa atcattaccg tcgatggcga
    16801 tgaaattggt gagcaccagg ggctgatgta tcacactctc ggtcagcgta aaggtctggg
    16861 tatcggtggc accaaagaag gtaccgaaga accgtggtat gtggtggaca aagacgtcga
    16921 aaacaacatt ctggttgtcg ctcagggcca tgaacacccg cggctgatgt ctgtcgggtt
    16981 gattgcccag cagttgcact gggtcgatcg cgaaccattc accggcacta tgcgttgcac
    17041 ggtaaaaacc cgctatcgcc agaccgatat cccttgcacc gtcaaggcgc tggacgatga
    17101 tcgcattgaa gtgattttcg atgaaccggt tgccgccgtg acgccgggcc agtctgccgt
    17161 cttctataac ggtgaagtgt gcctcggtgg cggtattatt gagcagcgtc tgccgctgcc
    17221 ggtctgatta ttatctttac ttaaaacaag gaagcagtga acgtggcaaa gaattactat
    17281 gacatcaccc tcgccctggc cggtatttgt cagtcggcac gcctggtgca acaactcgct
    17341 caccaggggc attgtgatgc cgatgcgcta cacgtctcac tcaacagtat tattgatatg
    17401 aaccccagct cgacgctggc ggtttttggc ggtagcgaag ccaacctgcg cgtcgggctg
    17461 gaaaccctgc tcggcgtgct caatgccagc agtcgccagg gcttaaacgc cgaattaacc
    17521 cgctacacac tcagcttgat ggtgcttgag cgcaaactct cctcagcgaa aggcgcgctc
    17581 gacactctgg gcaaccggat caacggcctg caacgccagc tcgaacactt cgatttacag
    17641 tccgaaacgc tgatgagcgc gatggctgct atctatgttg atgtgatcag cccgcttggc
    17701 ccgcgcattc aggtcaccgg ttcccctgct gtactgcaaa gcccacaagt gcaggcgaaa
    17761 gttcgcgcaa ccctgctggc aggcattcgc gccgccgtgc tctggcacca ggtcggcggc
    17821 ggacgtctgc aactgatgtt ttctcgtaat cgcctgacca ctcaggcaaa acaaattctt
    17881 gctcatttaa ccccggagtt gtgatctatg gaattatcct cactgaccgc cgtttcccct
    17941 gtcgatggac gctacggcga taaagtcagc gcgctgcgcg ggattttcag cgaatatggt
    18001 ttgctgaaat tccgtgtaca agttgaagta cgttggctgc aaaaactggc cgcgcacgca
    18061 gcgatcaagg aagttcctgc ttttgctgcc gacgcaatcg gttaccttga tgcaattgtc
    18121 gccaatttca gcgaagaaga tgccgcacgc atcaaaacca tcgagcgtac cactaaccac
    18181 gacgttaaag cggttgagta tttcctgaaa gaaaaagtgg cggagatccc ggaactgcac
    18241 gcggtttctg aattcatcca ctttgcctgt acttcggaag atatcaataa cctctcccac
    18301 gcattaatgc tgaaaaccgc gcgtgatgaa gtgatcctgc cgtactggct tcaactgatt
    18361 gatggcatta aagatctcgc cgttcagtac cgcgatatcc cgctgctgtc tcgtacccac
    18421 ggtcagccag ccacgccgtc aaccatcggt aaagagatgg caaacgtcgc ctaccgtatg
    18481 gagcgccagt accgccagct taatcaggtg gagatcctcg gcaaaatcaa cggcgcggtc
    18541 ggtaactata acgcccatat cgccgcttac ccggaagttg actggcatca gttcagcgaa
    18601 gagttcgtca cctcgctggg tattcagtgg aacccgtaca ccacccagat cgaaccgcac
    18661 gactacattg ccgaattgtt tgattgcgtt gcgcgcttca acaccattct gatcgacttt
    18721 gaccgtgacg tctggggtta tatcgccctt aaccacttca aacagaaaac cattgctggt
    18781 gagattggtt cttccaccat gccgcataaa gttaacccga ttgacttcga aaactccgaa
    18841 gggaatctgg gcctttccaa cgcggtattg cagcatctgg caagcaaact gccggtttcc
    18901 cgctggcagc gtgacctgac cgactccacc gtgctgcgta acctcggcgt gggtatcggt
    18961 tatgcgctga ttgcgtatca atccaccctg aaaggcgtga gcaaactgga agtaaaccgt
    19021 gaccatctgc tggatgagct ggatcacaac tgggaagtgc tggctgagcc aatccagaca
    19081 gttatgcgtc gctatggcat cgaaaaaccg tacgagaagc tgaaagagct gactcgcggt
    19141 aagcgcgttg acgccgaagg catgaagcag tttatcgatg gtctggcgtt gccagaagaa
    19201 gagaaagccc gcctgaaagc gatgacgccg gctaactata ttggtcgagc tatcaccatg
    19261 gttgatgagc tgaaataaac tcgtatcagt gccggatggc gatgctgtcc ggcctgttta
    19321 ttaaaattat ccactttttc ttttttcact ttacctcccc tccccgctgg tttatttaat
    19381 gtttaccccc ataaacacat aatcgcgtta cactatttta ataattaaga cagggagaaa
    19441 taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccac cttaaagttc
    19501 agattcagga tgctggtcat caggtcgatg acgcagaaga tgccaaagaa gccgattatt
    19561 atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcct gacgaggacg
    19621 gtctgtcact gattcgccgc tggcgtagca atgatgtttc actgccgatt ctggtattaa
    19681 ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgccggtgct gatgattacg
    19741 tgactaaacc gtttcatatt gaagaggtga tggcgcgaat gcaggcatta atgcggcgta
    19801 atagcggcct ggcttcacag gtcatttcgc tacccccgtt tcaggttgat ctctctcgcc
    19861 gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac accattatgg
    19921 aaacgttgat acgcaataat ggcaaagtgg tgagcaaaga ttcgttaatg ctccaactct
    19981 atccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga cgtctgcgca
    20041 aaaaaattca ggcacaatat ccgcaagaag tgattactac cgttcgcggc cagggctatt
    20101 tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct cactgcgggt
    20161 acgttttctg ttggcaacgg ctgcggtagt actggtgctt tcgcttgcct acggaatggt
    20221 cgcgctgatc ggttatagcg tcagtttcga taaaactacg tttcggctgt tacgtggcga
    20281 gagcaatctg ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg tcgagttacc
    20341 cgaaaatatc gacaagcaaa gccccaccat gacgctaatt tatgatgaga acgggcagct
    20401 tttatgggcg caacgtgacg tgccctggct gatgaagatg atccagcctg actggctgaa
    20461 atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct tgctgagtgg
    20521 agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg acgcggagat
    20581 gacccactcg gtggcagtaa acgtctaccc ggcaacatcg cggatgccaa aattaaccat
    20641 tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct ggagctggtt
    20701 tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg tcgccgcctg
    20761 gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg aagaacataa
    20821 ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac gaaacctgaa
    20881 ccgattgtta aaaagtgaac gcgaacgtta cgacaaatac cgtacgacgc tcaccgacct
    20941 gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt ctctgcgtag
    21001 tgaaaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca gccgcatttc
    21061 acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat tgctcagccg
    21121 cgagctgcat ccggtcgccc cactgctgga caatctcacc tcagcgctga acaaagtgta
    21181 tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct ttgtcggtga
    21241 gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta aatattgcct
    21301 cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg tggtcgagga
    21361 tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg gtcaacgggt
    21421 tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa tcaccgagca
    21481 atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga tggaggtgat
    21541 ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtccatact tcacgcatta
    21601 cgttaagcat ccgttataat cggttgcaga taccagcctg tggatgctta acatggaata
    21661 ccaactcact cttaactggc ccgattttct tgaacgtcac tggcagaaac gcccggtggt
    21721 gttaaaacgc ggctttaata attttattga cccgatctct ccagacgagt tggcgggtct
    21781 ggcgatggaa agcgaagttg acagtcgact ggtcagtcac caggatggca aatggcaggt
    21841 cagccacggc ccgttcgaaa gctacgatca tctcggtgaa accaactggt cattactggt
    21901 acaggcagtg aaccactggc atgagccgac cgccgcgctg atgcgaccgt tccgtgaact
    21961 accggactgg cgtattgatg atctgatgat ctctttttct gtaccgggcg gcggcgtcgg
    22021 cccgcatctc gatcagtacg acgtgtttat cattcagggt accggtcgtc gtcgctggcg
    22081 agtgggcgaa aagctgcaaa tgaaacagca ctgtccgcac ccggatctgt tacaggtcga
    22141 tccgttcgaa gccatcatcg atgaagagct ggagcctggc gatattcttt atattccgcc
    22201 aggattcccg catgaaggct acgcgctgga aaatgcgatg aactattccg tgggttttcg
    22261 cgcgccgaat acgcgggaac tgattagcgg atttgccgat tatgtgctgc aacgtgaact
    22321 gggcggcaac tactacagcg atccggatgt tccacctcgc gctcatcctg cggatgttct
    22381 gccgcaagag atggataaac tgcgtgagat gatgctcgaa ttgatcaacc agccggaaca
    22441 ctttaagcaa tggtttggcg agtttatatc ccagtcacgt catgaactgg atatcgcgcc
    22501 gccggaaccg ccttatcagc cagatgaaat ctacgatgcg ctgaaacaag gtgatgtgct
    22561 ggtgcgcctg ggtggtctgc gcgtattgcg cattggcgac gacgtgtatg ccaatggtga
    22621 gaagatcgat tccccgcacc gtccggcact ggatgcactc gccagcaaca ttgcgctgac
    22681 tgcggagaat tttggcgatg cgctggaaga tccgtcattc ctcgcaatgc tcgcggcgct
    22741 ggtaaatagc gggtattggt tcttcgaagg gtaagtttgg atttaagccg gatgtggtgc
    22801 tgattgcgcc ttatcgtgcc tacatgcact gaacgcctgt aggcacgata agtatgtgga
    22861 ccagtgtcaa tataacggtt tcatcccggt aattggcagt ccacatacgc atcgggcaat
    22921 cacgacttcc gttgcgccgt taactcggca atacggacga tcacctgcac cgctttttcc
    22981 ataccttcca gagtcacaaa ctcatgctta ccatgatagt tgtaaccgcc agtgaacagg
    23041 ttcgggcaag gtaatcccat aaacgacaac tgcgcgccgt cggtaccgcc gcggatcggt
    23101 ttcagttccg gttcaatatc gcagtcacgc atcgcctgct gggcgatatc gagaatatgc
    23161 ggatgctcaa ccactttctc gcgcatattg tagtaactgt cttcaatcac caattcaatg
    23221 tagcaatcag gatgtaaccc tttgcccact tttttggcga tctccatcat tttacgttta
    23281 cgcgcttcaa actgtttacg gtcgaaatca cggatgatgt aatgcatatc ggcccgttca
    23341 acggtgcctt tcatgctcgc caggtgatag aaaccttcat agccttctgt catttccggg
    23401 ctttcatccg ccggaacttc cgcatgaata cgtgccgcca gcgacagcgc atttaccatc
    23461 actcctttcg ccgtgcccgg gtgaacattg ttaccgacaa ttttgatatt gaccgacgcg
    23521 gcgttgaagt tttcaaactc cagttcgcct acgccaccac catcaacagt gtaagcccag
    23581 cgggcatcga aggcgtcaac atcaaaatgt ttcgcccctt tgcccacttc ttcatccggg
    23641 gtaaaggcga cgcgaatatc accatgcgga atgtttttct gttgcaatac cgccagcgcg
    23701 gtcatgattt ctgcaatacc tgctttgtca tcggcaccta acaaggtttt accatcggta
    23761 gtaatcagcg tctgacccag tagctgatgc agcaccggga acataaccgg tgataaaact
    23821 tcatcgccga tacccagcgc aatatcgcca ccgcgatagt tttcaacaat ttgcggattc
    23881 acatttttgc cgctgcaatc cggtgaggta tccacatgag aaataaagcc aatcgccggg
    23941 atattgccag ggacgttagc cggtaacgtc gccatcaaag tgcccttctc acttaaggtc
    24001 acattgataa gccccatctc ttcgagctgc tctttcagta gatgcaataa cttccattgg
    24061 ccttccgtgc tgggaacctg tctcacccct gcttttgatt gggtatccag agacacgtag
    24121 ttcaaaaatc gctcaagtag tttatccatg tagtcaccct cactttttgt gacaacatta
    24181 ttaagaagca agaaaagaca aatattgcgt caggtcactt ttacccctgc aagcgggaat
    24241 atttatcagc attaccttaa tgaatataaa gctaagccag taattcacaa caaagattgg
    24301 cgattcaagc ggtttgccgt agaattaccg ccctcattaa gagtcaccaa ggtggttaac
    24361 cacaaacccc gcatcggtaa gccatccgtt gcgtttacat gggacagagt aaaaaattga
    24421 ataaacaacc gagttcgctt tcaccgctgg tgcaattggc gggaattcgc aaatgctttg
    24481 atggtaaaga ggtcattccc cagctggatc tgactatcaa caatggcgag ttcctcacgc
    24541 tgcttggccc ttctggctgc ggtaaaacaa ccgttcttcg cctgattgca ggtctggaaa
    24601 ctgttgattc cggacgcatc atgctggata acgaggacat cacccacgtt ccggcggaaa
    24661 accgctatgt gaacactgtt ttccaaagct acgcactttt cccccacatg accgtgttcg
    24721 aaaatgtggc ctttgggttg cgcatgcaaa aaacccccgc tgctgaaatt acgccccgcg
    24781 ttatggaagc cctgcggatg gtgcagctgg aaaccttcgc tcaacgtaaa ccgcatcagc
    24841 tctctggtgg tcaacagcaa cgcgtcgcca ttgcccgtgc ggtggttaac aagcctcgtc
    24901 ttttgttgct ggatgagtcg ctctcagcgc tggattacaa actgcgtaag caaatgcaga
    24961 acgagctgaa agcgttacag cgtaagcttg gcattacatt cgtcttcgtg actcacgacc
    25021 aggaagaagc actcaccatg tcagacagga ttgtggtgat gcgcgatggt cgcatcgagc
    25081 aagacggcac gccgcgtgaa atctacgaag agccgaaaaa cctgtttgtt gccgggttca
    25141 ttggcgaaat caatatgttt aacgcaacgg tcatcgaacg tctcgacgag cagcgcgtac
    25201 gcgccaacgt tgaaggccgc gaatgtaata tctacgttaa cttcgccgtt gaaccggggc
    25261 aaaaactgca tgttctgctg cgcccggaag acttacgtgt tgaagagatt aacgacgaca
    25321 accacgctga agggctaatt ggttacgttc gcgagcgtaa ctacaaaggc atgacgctgg
    25381 agtcggttgt tgaactggaa aatggcaaga tggtgatggt cagcgaattc ttcaatgaag
    25441 acgatcctga ctttgaccac tctctcgacc aaaaaatggc cattaattgg gtagaaagct
    25501 gggaggtcgt actggctgat gaagaacaca agtaagttcc agaatgtagt gattgtcact
    25561 attgtcggtt ggcttgtgtt gtttgtcttt ctgcccaacc tgatgatcat tggcaccagc
    25621 tttttgaccc gcgacgacgc cagtttcgtc aaaatggtct ttacgctgga taactatacg
    25681 cgtctgctcg atccgctcta ttttgaagtg ctattgcact cgctgaatat ggcgctgatc
    25741 gccaccctcg cctgcctggt gctgggctac ccgtttgcct ggtttctggc gaagctgcca
    25801 cacaaggtgc gtccgctgct gctgtttctg ctgattgttc ccttctggac caactcattg
    25861 attcgtatct acgggctgaa aattttcctc agcaccaaag gctatctcaa cgagtttttg
    25921 ctctggctgg gcgttataga tacaccaatt cgcatcatgt tcacgcccag tgcggtgatt
    25981 atcggtctgg tttacattct gctgccgttt atggtgatgc cgctgtattc cagtatcgaa
    26041 aaactggata agccgttgct tgaggcagcg cgcgatctcg gtgccagcaa gttacagact
    26101 tttatccgta tcattattcc actgacgatg ccgggaatta ttgccggatg tctgctggtg
    26161 atgctgccag cgatgggcct gttctatgtg tccgacctga tgggcggtgc gaaaaacctg
    26221 ctgatcggta acgtcatcaa ggtacagttc ctcaatattc gtgactggcc gtttggtgca
    26281 gccaccagca ttacgctgac tatcgtaatg ggcctgatgt tgctggttta ctggcgcgct
    26341 tctcgtctgc tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt cgcggcggtt
    26401 ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt gtgaactcct
    26461 ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg tatagcctgc
    26521 tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg gcggtgtttt
    26581 cggcgacatt tgctacgctt atcggttcac tgacggcagt tgcgctgtac cgttatcgct
    26641 ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca ccagatatcg
    26701 tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt ggcttctggt
    26761 cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg gtgtattcgc
    26821 gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt gccagtgaat
    26881 ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg gcgggctggg
    26941 tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcgttcgtc accggaccaa
    27001 gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta tcgccggaag
    27061 ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt gccagccagc
    27121 ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa aatggtcacg
    27181 ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg ccgatgacaa
    27241 caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc ttgaacagtt
    27301 caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg aaaccatgta
    27361 cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt caacctatta
    27421 cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga agttaacgaa
    27481 cttcagcaat ctcgatccag acatgctcaa caagccgttt gacccgaata acgattactc
    27541 cattccgtat atctggggtg cgacggcgat tggtgttaac ggtgatgcgg tggatccgaa
    27601 atctgtcacc agctgggccg atctgtggaa gcctgagtac aaaggcagcc tgctgttgac
    27661 cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact ccggtaacac
    27721 caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga tgccaaacgt
    27781 cgcagcgttt aactccgata acccagctaa cccgtacatg gaaggcgaag ttaacctcgg
    27841 catgatctgg aacggttctg ctttcgttgc acggcaggcg ggtacgccaa ttgacgtggt
    27901 gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg caaatgccaa
    27961 aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg tggcaaaaca
    28021 ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc tgttaagccc
    28081 ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa atggtgaatg
    28141 gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga agctgaaagc
    28201 aggacgttaa ttcagcaagc gggcggtggc attaccgccc gttttttatt tagcaaggat
    28261 gtggctaatg agtcaggaca gtaaagtgtt atcccgcgct tttctcggta tcggtctgat
    28321 attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca aaagcgcggt
    28381 tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa acgacgtcga
    28441 tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt acacgctaat
    28501 tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca gagtcaatgt
    28561 ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg ttaatttttt
    28621 taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc tgataatcac
    28681 gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc cgcccatgaa aaaattcatt
    28741 atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc attgattcca
    28801 ataatgaaga cactttttac ggatgttacc ggtactgtac cggatgctga agagatcgca
    28861 cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt gattttaccc
    28921 gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc gcatgcgtca
    28981 tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg cgaaccacgg
    29041 ttaattatga tcaccaacgg ctaggttgaa agtggtctgg acgataaaca tctcggcctt
    29101 ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca gggaatcaat
    29161 aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc actgacgtat
    29221 tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa cacattaggt
    29281 tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta cacgactcgt
    29341 tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg ttatcaggct
    29401 ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact ctgggcaggg
    29461 ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg ataaaagagg
    29521 ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc ggcccacaaa
    29581 acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc gcatcaggca
    29641 atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca ccacctggct
    29701 tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt cgagattcag
    29761 ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg ccggataaac
    29821 atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca tataaatttc
    29881 atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg gtgccgggaa
    29941 ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga gaacctgacc
    30001 actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat tggtattacc
    30061 tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat cgccgagggc
    30121 gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg gctgctgcag
    30181 ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat cgaaaccttc
    30241 cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt
    30301 acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc ttggtttttc
    30361 cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag
    30421 gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac
    30481 ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc
    30541 gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac gaggcagcct
    30601 gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa tgacgaccag
    30661 gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat ccagataacg
    30721 ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa tttcgggagc
    30781 ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac cagacagata
    30841 attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat ccaaccccat
    30901 catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa tgtagctttt
    30961 accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc cgagaatcaa
    31021 ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa gggcaaaaca
    31081 gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag
    31141 cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt ctgtttccgg
    31201 cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt cagcttcagc
    31261 taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg
    31321 cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt ttgttccacc
    31381 aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt tactggccgc
    31441 taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa cttgccaaaa
    31501 gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc aattccgatg
    31561 gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc aacttttcaa
    31621 tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc accccacaca
    31681 ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga attaaaccat
    31741 ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc accgccatca
    31801 ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc gccagataca
    31861 taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca atccaacttt
    31921 taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta ttggcgttga
    31981 aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca agatattgtt
    32041 gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc aactgcaaaa
    32101 taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg ggattcgagt
    32161 tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc gccacgcctt
    32221 tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg ccctggacaa
    32281 acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc acctggattg
    32341 cgcgaagatt cacgccactt tccaccagcc cggtgaaatt gatatacggc gcggcagcgg
    32401 caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc cacggctgat
    32461 ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc agttcgcgtt
    32521 caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg gcaatgccaa
    32581 tggtagaaat aacggagatc agcgacacca tgccgccgcg ccgccgtccg cgactaaaac
    32641 gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca ggctcagttc
    32701 cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg ccagttgcag
    32761 gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc caagcaactg
    32821 gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg ccagtaccag
    32881 gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc cgccagaaag
    32941 ttcagatggg cggtgattcg cacgatgatc cagccccacc gcttttaaca tctcaagtgc
    33001 acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag ccacgttttc
    33061 cagggcggta aaatccggca gcag
//
LOCUS       DAFATA010000044        32281 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFATA010000044 DAFATA010000000
VERSION     DAFATA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.045"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..488)
                     /locus_tag="LGJ20_002491"
     CDS             complement(<1..488)
                     /locus_tag="LGJ20_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HBK2386541.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFA"
     gene            complement(598..1521)
                     /locus_tag="LGJ20_002492"
     CDS             complement(598..1521)
                     /locus_tag="LGJ20_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK2386542.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(1523..2170)
                     /locus_tag="LGJ20_002493"
     CDS             complement(1523..2170)
                     /locus_tag="LGJ20_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBK2386543.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(2181..2765)
                     /gene="rimJ"
                     /locus_tag="LGJ20_002494"
     CDS             complement(2181..2765)
                     /gene="rimJ"
                     /locus_tag="LGJ20_002494"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBK2386544.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            3001..4209
                     /gene="mdtH"
                     /locus_tag="LGJ20_002495"
     CDS             3001..4209
                     /gene="mdtH"
                     /locus_tag="LGJ20_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBK2386545.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            4273..4920
                     /gene="grxB"
                     /locus_tag="LGJ20_002496"
     CDS             4273..4920
                     /gene="grxB"
                     /locus_tag="LGJ20_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBK2386546.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            5054..5614
                     /locus_tag="LGJ20_002497"
     CDS             5054..5614
                     /locus_tag="LGJ20_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2386547.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            5720..6766
                     /gene="pyrC"
                     /locus_tag="LGJ20_002498"
     CDS             5720..6766
                     /gene="pyrC"
                     /locus_tag="LGJ20_002498"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK2386548.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            6840..7085
                     /gene="dinI"
                     /locus_tag="LGJ20_002499"
     CDS             6840..7085
                     /gene="dinI"
                     /locus_tag="LGJ20_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBK2386549.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            7375..7629
                     /gene="bssS"
                     /locus_tag="LGJ20_002500"
     CDS             7375..7629
                     /gene="bssS"
                     /locus_tag="LGJ20_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBK2386550.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            7744..8862
                     /gene="solA"
                     /locus_tag="LGJ20_002501"
     CDS             7744..8862
                     /gene="solA"
                     /locus_tag="LGJ20_002501"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBK2386551.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLFITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            8910..9023
                     /locus_tag="LGJ20_002502"
     CDS             8910..9023
                     /locus_tag="LGJ20_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBK2386552.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            9284..9850
                     /locus_tag="LGJ20_002503"
     CDS             9284..9850
                     /locus_tag="LGJ20_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBK2386553.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            9854..10429
                     /locus_tag="LGJ20_002504"
     CDS             9854..10429
                     /locus_tag="LGJ20_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBK2386554.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(10471..11523)
                     /locus_tag="LGJ20_002505"
     CDS             complement(10471..11523)
                     /locus_tag="LGJ20_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK2386555.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            11748..12668
                     /gene="lpxL"
                     /locus_tag="LGJ20_002506"
     CDS             11748..12668
                     /gene="lpxL"
                     /locus_tag="LGJ20_002506"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBK2386556.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            12840..14066
                     /gene="mdtG"
                     /locus_tag="LGJ20_002507"
     CDS             12840..14066
                     /gene="mdtG"
                     /locus_tag="LGJ20_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HBK2386557.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            14149..14523
                     /locus_tag="LGJ20_002508"
     CDS             14149..14523
                     /locus_tag="LGJ20_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBK2386558.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(14524..14751)
                     /locus_tag="LGJ20_002509"
     CDS             complement(14524..14751)
                     /locus_tag="LGJ20_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBK2386559.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(14924..17467)
                     /gene="mdoH"
                     /locus_tag="LGJ20_002510"
     CDS             complement(14924..17467)
                     /gene="mdoH"
                     /locus_tag="LGJ20_002510"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBK2386560.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(17460..18995)
                     /gene="mdoG"
                     /locus_tag="LGJ20_002511"
     CDS             complement(17460..18995)
                     /gene="mdoG"
                     /locus_tag="LGJ20_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBK2386561.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            19389..20546
                     /gene="mdoC"
                     /locus_tag="LGJ20_002512"
     CDS             19389..20546
                     /gene="mdoC"
                     /locus_tag="LGJ20_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBK2386562.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(20554..22035)
                     /gene="clsC"
                     /locus_tag="LGJ20_002513"
     CDS             complement(20554..22035)
                     /gene="clsC"
                     /locus_tag="LGJ20_002513"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBK2386563.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDVMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(21977..22510)
                     /gene="ymdB"
                     /locus_tag="LGJ20_002514"
     CDS             complement(21977..22510)
                     /gene="ymdB"
                     /locus_tag="LGJ20_002514"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBK2386564.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRSAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(22605..22915)
                     /locus_tag="LGJ20_002515"
                     /pseudo
     CDS             complement(22605..22915)
                     /locus_tag="LGJ20_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(23036..23368)
                     /gene="csgC"
                     /locus_tag="LGJ20_002516"
     CDS             complement(23036..23368)
                     /gene="csgC"
                     /locus_tag="LGJ20_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBK2386565.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(23427..23882)
                     /gene="csgA"
                     /locus_tag="LGJ20_002517"
     CDS             complement(23427..23882)
                     /gene="csgA"
                     /locus_tag="LGJ20_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBK2386566.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(23923..24378)
                     /gene="csgB"
                     /locus_tag="LGJ20_002518"
     CDS             complement(23923..24378)
                     /gene="csgB"
                     /locus_tag="LGJ20_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBK2386567.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            25132..25782
                     /gene="csgD"
                     /locus_tag="LGJ20_002519"
     CDS             25132..25782
                     /gene="csgD"
                     /locus_tag="LGJ20_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBK2386568.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            25787..26176
                     /gene="csgE"
                     /locus_tag="LGJ20_002520"
     CDS             25787..26176
                     /gene="csgE"
                     /locus_tag="LGJ20_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBK2386569.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            26201..26617
                     /gene="csgF"
                     /locus_tag="LGJ20_002521"
     CDS             26201..26617
                     /gene="csgF"
                     /locus_tag="LGJ20_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBK2386570.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            26644..27477
                     /gene="csgG"
                     /locus_tag="LGJ20_002522"
     CDS             26644..27477
                     /gene="csgG"
                     /locus_tag="LGJ20_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBK2386571.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(27541..28032)
                     /locus_tag="LGJ20_002523"
     CDS             complement(27541..28032)
                     /locus_tag="LGJ20_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBK2386572.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(28134..28688)
                     /gene="ycdY"
                     /locus_tag="LGJ20_002524"
     CDS             complement(28134..28688)
                     /gene="ycdY"
                     /locus_tag="LGJ20_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBK2386573.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(28712..29449)
                     /gene="ycdX"
                     /locus_tag="LGJ20_002525"
     CDS             complement(28712..29449)
                     /gene="ycdX"
                     /locus_tag="LGJ20_002525"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBK2386574.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(29504..30442)
                     /gene="ghrA"
                     /locus_tag="LGJ20_002526"
     CDS             complement(29504..30442)
                     /gene="ghrA"
                     /locus_tag="LGJ20_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBK2386575.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            30676..30763
                     /locus_tag="LGJ20_002527"
     tRNA            30676..30763
                     /locus_tag="LGJ20_002527"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30710..30712,aa:Ser,seq:gga)
     gene            complement(30956..31942)
                     /locus_tag="LGJ20_002528"
     CDS             complement(30956..31942)
                     /locus_tag="LGJ20_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386576.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
     gene            complement(31939..>32281)
                     /locus_tag="LGJ20_002529"
     CDS             complement(31939..>32281)
                     /locus_tag="LGJ20_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK2386577.1"
                     /translation="AWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVAPSI
                     IKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRVMHC
                     RKAFSDAKVFK"
BASE COUNT         8312 a         8158 c         7587 g         8224 t
ORIGIN      
        1 gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac cagcgacgcc
       61 agggagatca gcaagatata gggaaaggta atctttagta gctggctggt cagggcaaat
      121 ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc
      181 atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac ataagagaca
      241 aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat cggtacaaat
      301 gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag tttaaaagcg
      361 acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat tgcgtctcgt
      421 gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag cgattttaat
      481 aaattcatcg gtgttctaat ccagacccac gggcaaaacg cctgcaaggc aggcgctgaa
      541 agaggcgaat agtctacctg gattatggtg aattgctacc gccagatgtt acagggttta
      601 ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca cggcctgttc
      661 gccggcggtt tgcggaactg tctgattttg cacacattca atgaagtgcc gcgcacagcc
      721 gacaaatcca cgttgttcaa gagtgctctg ccaaccagga atcggtttat gcactacgcc
      781 ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg caccgtcagt
      841 cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacagg tggtgatctg
      901 caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag
      961 tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg tatccaccac
     1021 atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacac tattgctacg
     1081 atgtttatcc attcttagcg aagctgcggt agcgagttgc gtttttaact caccgtagag
     1141 tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttacgcg ccgccagttc
     1201 caccagccgt tcagcatcgc gcagattttc tgccagcggt ttatcgacac agacatgtac
     1261 acccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg aatgcacaaa
     1321 aaccgcatcg caactggcgg caaggctgga taacgaatcg gcataaggaa tgcgccagct
     1381 ttcacaaatt ggcagggctt tcgcgcgcgt aggcgaccag gctccttgta acatccagtc
     1441 agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta atcccacaac
     1501 gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa tcaagacgct
     1561 gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggcctgtaaa tcaccatcaa
     1621 ccgcgttcga catatccgtt accgccggct gatcctctac ctcaccactg aaaagatgca
     1681 tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa ggaccatctt
     1741 cgcgatttgc cagttgttcc agcgtcgact ccacttccgc catatcgctg aattcataca
     1801 ttcgcgcggc gcggctgcgc agttcacctg gcgtctgggc accacgcaat aacaacgtgg
     1861 tgattaacgc cacttccgct gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac
     1921 gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt
     1981 tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg accggttcac
     2041 ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg tattgctccg
     2101 gcgtggtcac ctgcttttcc agcaagcaac caatcacgcg agcttcaagg gcagtaagtt
     2161 gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt taatgccgtc
     2221 agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata gccttctttt
     2281 tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat ataattagcc
     2341 ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt cagggcttca
     2401 aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata gcaggcatga
     2461 aaagagccac gaacaacatt ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg
     2521 tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat catccccagc
     2581 ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc ccagggcttg
     2641 aggaaatggc gattctctgc gtaataatcc gcaagacgcc aggcatcacg atcatgcacc
     2701 agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact gcgatagcca
     2761 aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta ttactatacc
     2821 tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt cacatatttg
     2881 ataaaaactc tctatcaaag cgacgttttg ataaaaaaat attgtcccct aagtgatggg
     2941 aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc gggaggggaa
     3001 atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat cgataatatg
     3061 ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa
     3121 atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt tattcagcaa
     3181 ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt
     3241 accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca cgaaccgtgg
     3301 ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga tccgccgcgt
     3361 tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg
     3421 atgatgcagg acagtgccgg tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa
     3481 tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctgtg tgcggcgttc
     3541 aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc
     3601 atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct ggcgggttac
     3661 tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga cgtggctggc
     3721 gcaccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc
     3781 taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt gatggctggg
     3841 ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct gcaacaactt
     3901 ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc
     3961 ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt tagccgtctg
     4021 ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt tgacctgggc
     4081 aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg catcttcact
     4141 ttccttgcgc tgggttggca gtttagtcag aaacgtgccg cgcgtcgttt gctggaacgc
     4201 gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact gacagccaaa
     4261 tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct caaagcccgc
     4321 atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa cgacgacgca
     4381 gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa agatgacagc
     4441 cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga cggcaaaccg
     4501 ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt caatggctac
     4561 gcaaacaaac tgctgttgcc gcgttttgcc aaatcggcat tcgatgagtt ttctactccc
     4621 gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt tgccgacctg
     4681 ctggcccact ctgacggtct gattaagaat atcagcgatg atttacgtgc gctggacaaa
     4741 ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat tcagctattt
     4801 ccgctgctgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg cgttgctgat
     4861 taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat ggcgatttaa
     4921 tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt tttttctgct
     4981 ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg cagatttcat
     5041 gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca gtggcctgct
     5101 cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta accagtcgct
     5161 tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg acgcccatat
     5221 tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg ttaccctaac
     5281 cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga ccatgaaact
     5341 gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat
     5401 ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct
     5461 tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt acgtcctgcg
     5521 cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa
     5581 gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc gcgcccttta
     5641 tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa acatcccttc
     5701 agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc gccgcccaga
     5761 cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc cgtataccag
     5821 cgaaatttat ggacgggcta ttgtaatgcc caatctggct ccgcccgtga ccactgttga
     5881 ggctgccgtg gcgtatcgcc agcgtattct tgatgccgta cctgccgggc acgatttcac
     5941 cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg agcgcggatt
     6001 taatgaaggc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca ctaactccag
     6061 ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg
     6121 tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt ttgatcgtga
     6181 agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg cgctgaaagt
     6241 cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg
     6301 gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata tgctggttgg
     6361 tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc accaacaggc
     6421 attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg attctgcgcc
     6481 acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca acgccccaac
     6541 cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc actttgaagc
     6601 attctgttct gtaaacggcc cgcagttcta tgggttgccg gtcaacgaca cattcatcga
     6661 actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg acaccctggt
     6721 gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat cgtagcttcc
     6781 tgttgtcatt aggttatttt aactgtataa ataaccagta tattcaacag ggggctatta
     6841 tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct attgacgccc
     6901 tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa ggccacgtat
     6961 cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa gaagataaac
     7021 agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg
     7081 aataatatgc agtgattttt gtttttgccg ggtcgccccg gcttttttat tttacttaca
     7141 aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg ctaaaaaaat
     7201 atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt tatctgttcc
     7261 cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg cttcagataa
     7321 aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc catgatggaa
     7381 aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag caccgttgat
     7441 agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc cgagcaggaa
     7501 gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca atttatttcg
     7561 atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa cgagtatcgg
     7621 cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc tttaccattt
     7681 ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag cagagagaaa
     7741 aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc cgccgggtat
     7801 tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc accgcatcaa
     7861 cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga aggcgaaaag
     7921 tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa
     7981 gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga ctccgcattt
     8041 ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa gctcgatgcg
     8101 caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat cggcttattt
     8161 gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat ccaactggcg
     8221 aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg tcatgacgat
     8281 gatggcgtaa ctattgaaac cgttgacggt gagtatcagg cgaaaaaagc gattgtctgc
     8341 gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt acgcaaagta
     8401 tttgcctggt atcaggccga tgggcgctat agcgtgaaga ataaattccc ggcgtttacc
     8461 ggtgaacttc ccaatggcga tcaatattat ggttttccgg cagaaaacga cgcgttgaag
     8521 attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg
     8581 gaagtggtca gcgatggttc ggaagccttc ccgttcttgc gcaatgtatt gccgggtatc
     8641 ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga agattttatt
     8701 atcgataccc tacccgacca cgataataca ctgttcatta ccggcctgag tgggcacggt
     8761 tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga caaaaaaagc
     8821 gattttgatt taacgccatt caggctttcc cgcttccaat aatcatcatt acggcctcca
     8881 gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat ctcatcaata
     8941 atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca attatggatc
     9001 tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa tatttaatct
     9061 ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag cactcaccag
     9121 acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta attcaaaatt
     9181 attgaaataa aaataaatat cattcaattt ggtatttttg tgttgcaatt cattctccga
     9241 cacgccattt tctttctctc gcaaattcgt tttggatgca cttatgtcat tcacaaatac
     9301 ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa ttattgtcta
     9361 cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg gctggtatca
     9421 caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc tggttattcg
     9481 cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt cgcccatgac
     9541 tcgtcttgcc gcaaaagctg gacatctcgc attgtaccta ttgctgttcg ccatcggcat
     9601 cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg gctggtttga
     9661 cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg ccctgcattt
     9721 ttggctcgcg tggagcgtcg tagtactgtc cgttatgcac ggatttatgg ccctgaagca
     9781 tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt catctgacta
     9841 tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc
     9901 tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc acgcctttgt
     9961 taatttccgc atccagcacc ttggctatag ctggttatac ggcaccttta aagatttcga
    10021 cggtactttt acctttgacg aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa
    10081 caccaccagc gtcgatacta atcacgccga acgcgataaa catcttcgca gtgcagattt
    10141 cctcaatacc gcaaaatatc cacaggcaac attcacctcc accagcgtga agaaagacgg
    10201 tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct
    10261 ggaagcgaaa ttaattggtc agggcgacga cccatggggt ggtaaacgtg caggcttcga
    10321 ggccgaaggc aaaattaagc tcaaggactt caatatcaag acagatttag gtccagcttc
    10381 tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag agtgaaatgt
    10441 tgccggatgg cacgcatccg gcaatgatat ttattccgtt ggatcaggaa tgcccagtgt
    10501 tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac gtcctgcccg
    10561 acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct cactacaaca
    10621 acctttgtat ttttccgcac atactggaca ctgaataaac agcaggtggc agccatcatt
    10681 tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg cgataatctc
    10741 atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac
    10801 cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct cgatatgcca
    10861 gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac gaatgccgcc
    10921 ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga ctgcttttgg
    10981 cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc
    11041 atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt
    11101 cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag gatcgtcaat
    11161 accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg atttcccgtc
    11221 atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg
    11281 cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg
    11341 cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata aagcgtcacg
    11401 ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc
    11461 gctctcagcc aacattttgg cttttagcgc gtcgttggaa atgcggttgt gtaacactgg
    11521 catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat atagcaatta
    11581 tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt atttgttatt
    11641 tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat
    11701 gcgacaatac atacaattgc ccgaataggt tgaaaaacag gattgatatg acgaatctac
    11761 ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg ggtattggcg
    11821 tactttggtt agtcgtgcaa ttgccctacc cggttatcta ccgcctcggt tgtggattag
    11881 gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac
    11941 tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat
    12001 ccgttggcat gggcctgatg gaaaccggca tggcgtggtt ctggccggac cgccgaatcg
    12061 cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg
    12121 gcatcctgtt agttggcatc cattttctga cactggagct gggtgcgcgg cagtttggta
    12181 tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt gactggctac
    12241 aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca
    12301 tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accggatcat gattacggcc
    12361 cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc acgaccggaa
    12421 cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc
    12481 cagatggcaa agggtatcaa ttgattatgc tgccgccaga gtgttctccg ccactggatg
    12541 atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc atgatggcac
    12601 cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc gttccttcac
    12661 gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc acagcaaatc
    12721 cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg gcgcataatt
    12781 agtgtgctta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag cggattgcta
    12841 tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc gtcgcctggc
    12901 taggctgttt tcttaccggt gccgccttca gtctggtaat gcccttctta cccctctacg
    12961 ttgagcagct tggcgttacc ggtcactccg ccctgaatat gtggtccggt attgtcttca
    13021 gcattacatt tttattttcg gccatcgcct caccgttttg gggtggactc gccgaccgta
    13081 aaggccgaaa actcatgcta ttacgctctg ccctcggcat gggcatcgtg atggtgttga
    13141 tgggactggc acaaaatatc tggcagtttt tgatcctacg ggcgcttctt gggttacttg
    13201 gcggatttgt ccccaacgct aatgctctta tcgccacaca agtaccgcgt aataaaagcg
    13261 gctgggcgct gggtacgctc tccacaggcg gcgttagtgg tgcgttgctc ggcccaatgg
    13321 ctggcggcct gctcgccgat agctacggct tacgtccggt attctttatt accgccagtg
    13381 tgctcatact ctgctttttc gtcaccctgt tctgcatcag agaaaaattc cagccggtca
    13441 gcaaaaaaga gatgctgcac atgcgggaag tggtgacatc acttaaaaac ccgaaactgg
    13501 tactcagcct gtttgtcacc acgttaatca tccaggtagc gacaggctca attgccccca
    13561 ttctgacgct gtatgtccgc gaactggcgg gtaacgtcag taacgtcgcc tttatcagtg
    13621 gcatgatcgc ctcggtgcca ggcgtggcgg ctctgctaag cgcaccacga ctcggcaaac
    13681 ttggcgatcg aatcggaccc gaaaagatcc tgattacagc gctgatcttt tctgtactgc
    13741 tgttgatccc aatgtcttac gttcagacgc cattgcaact tgggatttta cgttttttgc
    13801 tcggtgccgc cgatggtgca ctactccccg ccgtacagac actgttggtt tacaactcga
    13861 gcaaccagat cgccgggcgt atcttcagct ataaccaatc gtttcgtgat attggcaacg
    13921 ttaccggacc attgatggga gcagcgattt cagcgaacta cggtttcaga gcggtatttc
    13981 tcgtcaccgc tggcgtagtg ttattcaacg cagtctattc atggaacagt ctacgtcgtc
    14041 gtcgaatacc ccaggtatcg aactgatttt tcgcctttca tacttgcaaa agcggagaat
    14101 cagctatcct tttccctgaa acctcatcaa ctcaaaggga gaatcgtgat gaccatgtac
    14161 gcaacgcttg aagaagccat tgacgctgca cgcgaagaat ttcttgcaga caaccccggc
    14221 atcgacgccg aagatgcgaa tgtgcaacag ttcaatgccc aaaaatacgt tttgcaggac
    14281 ggcgacatca tgtggcaagt tgagtttttt gccgacgaag gggaagaagg tgaatgttta
    14341 cctatgctta gcggtgaagc cgcgcaaagt gtttttgatg gcgactatga tgagatagag
    14401 atacgccagg agtggcagga agagaataca ttacatgaat gggacgaggg ggaatttcag
    14461 cttgagccac cgctggatac cgaggaagga cgcgcagcag ctgatgagtg ggatgaacgt
    14521 taatcactca tacgggccat gatgagtgtc gatcggcagc agtaaagtat caaaaaccag
    14581 agagaatggc agatcaagga tcgtgacata acgccaggcg gagtcacgca catcccattg
    14641 cacaccggga taatattggt tgccatgccc ctgccccggt atagtgcgac taataatgct
    14701 gccacagcca ctcagcaagg tcaccatgat gctcaccaca attaaacgca tttgatacct
    14761 ctttcgtaaa aaaataccgg cataacgcct gtatttttat tggctcgttg tttttgccgg
    14821 atgcggcgtg aacgccttat ccgacccaca aaatcttgca aaatcaataa attgcaggaa
    14881 cgatgtaggc ctgataagcg tagcgcatca ggcaactacg tttttattgc gaagccgcat
    14941 ccggtttacg caatgccagt ggattgagct ttatcccttc gtaataactc acccatgaag
    15001 aatatctctc cggggaattc cagacgcgga aatgcagacg ggccatcgtc accggatcgc
    15061 ttagcagcac caggcgacga tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt
    15121 caacgtggcg gtcacgggcg atttccagca ccttactggc gcggtgacgc gcggtcgcca
    15181 ttgcggttgc cagagcgtta aatgacgggt taaacactgc gtgcataaag ccatcatcaa
    15241 gggagcgttg acgattcatc tcaaggaacc gatcggtatc aaccagcacc tgcggcggcg
    15301 aatactcttc cgggatcagg aacagtttcc agcgtttggt gcgcagacca acggtggcac
    15361 ggctggaaat caccgaaaca aacggtgaca ggatcaacga gaagacaatc ggtgccagcc
    15421 agaacaggaa acgcagatcc aaccacgcca taccaacagc ccacactaac cctaacagca
    15481 gctgtgagcc gtggcgtttg aacgcttcac cccaggaagt ggagtcatca tcacgctgcg
    15541 gtgaattcca caccacttcc cagccaagga acgcgctgac aacgaagacc gtatggaaca
    15601 gcatgcgtac cggagccagc agcacggaaa aaagcacttc cagcagcaac gataatgtaa
    15661 cgcgccagaa gccgccgtat tctttcgttc ctttgcacca gataagcaaa atgctcaata
    15721 acttcggcag gaacaacagc accatagtcg aagcaaaaag tgcaatcgcc agctcaggac
    15781 gccactgcgg ccacaccggg aacaactgcc gtggttgcag gaagtattgc ggttcggtca
    15841 acgcatgtac tacctgcaat gcagtagaga gcgcgaggaa cataaaccac agcggagcgg
    15901 agagataaga catcacgccc gtcaggaata ccgcacggtg aaccgggtgc atacccttca
    15961 ccaggaacag acggaagttc atcaggttac cgtggcacca gcggcggtca cgttttagct
    16021 catcaagcaa gttaggcggc aattcttcat aagaacccgg gagatcgtaa gcaatccaga
    16081 ccccccaacc tgcacggcgc atcaacgccg cttccacgaa gtcatgtgac aggattgaac
    16141 cggcaaagga accttcgccc ggcagcggag ccagtgcgca gtgctcgata aacggtttca
    16201 cgcggataat cgcgttatgt ccccagtagt gcgactcgcc aagttgccag aagtgcaaac
    16261 cggctgtaaa cagtggccca tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg
    16321 tatccatacc ggacgctttc ggcgacgact gaatgatccc ggcgttcggg ttggcttcca
    16381 tcaggcgcac caggccgcat aaacaatcac cggtcattac cgagtcagca tccagcacca
    16441 ccatgtagct gtactggctg ccccagcgac ggcagaagtc atcgatatta ccgcttttac
    16501 gcttcacgcg gcgacggcgg cggcgataga aaatctgacc ttcgccaccg acttcagcga
    16561 taagctccat ccaggctttt tgctctgcga cgcagatatc cgggttataa ctgtcactaa
    16621 gaatgtagac atcaaagtgt ttggcattcc cggtggcttt tactgattcc cacgttgcac
    16681 gcaggccagc aaaaacacgg ttcacgtctt cgttacagat aggcatgatc aacgccgtgc
    16741 gatgctccgg gtttaatggt tcatcgccaa ctgttgacgc agatatactg tatttatcgc
    16801 gaccaataag cagttgcagg aagcccatta acgccgtcca gaatccggcg gacacccaac
    16861 agaacagtac cgcaaagagg atcaggatac cggtttgcag catataaggc agaagctgca
    16921 taaaggaaac ccacaaatcc tgaccaacca tatccatagg attaatcagc gcccaaccct
    16981 gataaggaag aatggtcttc atataccagg tcgcgacgac agtttgcgcg agcgtcagga
    17041 tcaacagaat gtaacggcgg atggtaccga cggtacgcca cttttgctcg ctctcctgct
    17101 cttctttggt caaacgagcc agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc
    17161 agaaacggcc taccgggttg gtacgccacg ggtcgggaaa catcgaggag cgttttgctt
    17221 ctggcatcgc cttcagctga tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa
    17281 gtgaatctgg ccaggcttgt tccagacgcg cctttaccga gccttgcggg gaatcatcct
    17341 cccgcgccca ggtgcggtgt tcggcatcca gcgcctgatg aacggcgcgg atatcagtct
    17401 tcggcaatgc cgctttctcg cttgcggcga tgggcattgc gtcaatgtac tcagttgtct
    17461 tattcattgg caggtaactg gtagctccag gtttcactca acgtctgatc ggcattcacc
    17521 agcgcagcac gcatttcagt ggttttcttg gcatctttca ctttcacacg catcaccaga
    17581 cgccagcctt tggtaaccgg gttatagcgc accgtgcttt caactatctc accattatca
    17641 ccaatgctgg tttgcgctgt gaccggggta tcctctggca gttttttcat ctctgcgccg
    17701 gtaaaatcga ccacaaaggc gatagtaccg tcaggctggc gaatcaggtt cgactgcttc
    17761 acatcccccg ttgaacgacg cgtttgttgc acccatgcgt tatctggcgc atgcagtttg
    17821 tcttcatcac ggctgaaggt gatggtgtat ttaaagttca tctctttacc cggctccggc
    17881 agctgatccg gcgtccagta agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt
    17941 tccaccagct caacgctgcc tttaccccac tcccctttcg gagtcaccca tgcgcttgga
    18001 cgaagatcgt aacgatcatc gagatcttca aagcgggaga aatcacgacc gcgctgcaac
    18061 agaccgaagc cttgcgggtt ctccatcgag aagctgctga ccgctaaatg tttcgggtta
    18121 ttcaacggac gccagatcca ttcgccatta ccggcatgga tagacagacc gttagagtcg
    18181 tgcaactccg gacgatagtt atttgcaggc gacggttggt tcggcccaaa caggaacata
    18241 ctggttaacg gtgcaacccc cagtttgccg actttatcgc gcagatagat tttcgactgc
    18301 acatccacaa ccgtgtcacg ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc
    18361 ggcgagtcaa gcaatgcata aatggttaaa cgtttatcag tcggttttgg acgctcgatc
    18421 cagaactctt tgaagcgtgg aaattcttca cccgatggca aggcggtatc aattgccagg
    18481 ccgcgggcag aaaggccata aacctgacct gcaccaatca cgcggaaata gctggccccg
    18541 agcatgctga cgatttcatc gtttttatct ttgctgttga tcgggtaaag cactttaaaa
    18601 ccggcaaaac caaggtcttt taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa
    18661 taatccgggc tgtatttgat tcgtttgact gcggtggcag tcacttcatt tattttgacc
    18721 ggggtatcga agtacatacc ctgatggtag aactcgagtt tgaatggggt cttcagattg
    18781 ttccagtacg ctttgtcatg attaaactgg atctgctgat agtccgcgta tttcatatcg
    18841 cggaaaacgg agggcaagtt gcttttgggc gcctcatagc ctttcccggc taaggattga
    18901 gcttgctttg cgacatcatc aatactgaaa gcccagcttg aagatgtata cagggttaac
    18961 attactgcag cactcaacca acgcattttc atcatttgta gtttatgttt cataattagt
    19021 aagcacttcc ccctttgtgt gcttatatcg atccgatcta ttttaatgga taaccagcca
    19081 ttccgacaac agaatcccgg tatcgttcag cacgctggct catggattaa gcacctgatt
    19141 gcaaccgttt tcactttccg tgcttctcct aaagacagaa gcatcgactt cgtgtagggt
    19201 ttgccagcaa ttttatttat caaccgtctt acagacgctt ccattctggt tagtggtagc
    19261 gatgatgcta tgcataaaaa ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct
    19321 atggcaacaa gcctaaaggc ataacccgac agggataaga cgaaaaatcc gagattagtt
    19381 aaccatatat gaatccagta cccgcgcaac gtgaatattt cctcgactcc atccgcgcct
    19441 ggctgatgtt gttagggatc ccttttcata tttctttaat ctattcgagc catacatggc
    19501 atgtgaatag cgccgaacca tcattgtggc tgaccctttt taatgacttc atccactcgt
    19561 tccgcatgca ggtatttttc gttatatccg gctacttttc ctacatgctt tttttacgct
    19621 atcccttgaa aaaatggtgg aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag
    19681 ccatccccct actgacatta ccgcaattta ttatgctgca atatgtcaaa ggaaaagcgg
    19741 aaagttggcc tgggctgtca ttgtatgaca aatataatac gttggcctgg gaattaatat
    19801 cacacctgtg gtttttactg gtgttagtag tcatgacgac gctgtgcgta tggatattta
    19861 agcgcatcag aaataattta gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa
    19921 aactatcggt gattttttta tgcctcggca tcggttatgc ggtaataaga agaacgattt
    19981 ttattgtgta tccgcccatt ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc
    20041 tattttattt accgttcttt atcctcggcg cactggcttt cattttccct catcttaaag
    20101 ccttgtttac cacgccgtct cgtggctgta ccctcgctgc agcattggcg tttgtcgctt
    20161 atttactcaa ccagcgctat ggcagtggcg atgcctggat gtacgaaacc gagtcagtga
    20221 tcaccatggt cctcggtctg tggatggtga atgtggtctt ctcctttggc caccgtttgc
    20281 ttaacttcca gtcagcgcgg gtgacttatt ttgttaacgc atcgctattt atctatctgg
    20341 ttcaccaccc gttaacgctg tttttcggcg catacattac accgcacatc acctccaact
    20401 ggcttggttt tctctgtggc ctgatattcg tagtagggat tgcgataatt ctgtatgaaa
    20461 ttcatttacg catcccgtta ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg
    20521 agaacgataa agcaccagcc cgttaagcca catttacaat aaccattcca cgggcaatat
    20581 cgacgccagt ctgaccataa cccgcttcca gaaactggtg gcgggttctt ttttgagaat
    20641 aatctctttc tttgcatgac gatcaaccca gttgatccgt ccccacctgt ccagacggag
    20701 ctgccaggcc gcatcatact ggctctgaat aaagcgttta tcaattaact gtgccagcgt
    20761 ttcgctctct atcacgaagc ccatttcagt attgagcaat gttgaacgcg gatcgaaatt
    20821 gaaagagccg ataaacaccg ttttaccatc gatgctaaag gttttagcat gcaggctggc
    20881 tccggaatta ccggttatgc cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt
    20941 gagttcatat aattccacgc catagcggag caattttttg cgccagcgcg cgtatccggc
    21001 atggacgaca gcaacatcgt tagcggcaag agaattggtt aggatcgcaa tctttacccc
    21061 ttttctcacc atccgtaaga gttgcgccac acctgcgcgt gtcggtacaa aataggaaga
    21121 gataatatcg atgcgttcac tgggtgagcc catgatatcg aacaggcgct gcggtaacag
    21181 tgaatgccgt tttgccttgc cctccccttt cgccggatca tcacttaata aacgtgtttt
    21241 cgcccagata agcggcaacg ttccatcaac cagatgattt ataaacggac tggattccat
    21301 tttgcgtaag taacgatgcg tcataacatc gttatgccag gaggcgggta actcgatgcg
    21361 atccgccatt tcaccttccg ggacatccag cacttgctgt aagggtgaaa ccgatttgca
    21421 ataccagtag cgggcgaaat catcggcaac gtcctctacc acgggcccta ttgccataac
    21481 atctaaatcc gaaaaaagtg gctcttcccc tgccccaaaa taggcatcac caatatttcg
    21541 cccccctacc agcgtcacca cgccatcgac agtgaaactt ttattgtgca tacggcgatt
    21601 gagacgggaa aagtcggtga tataaccaag cggacgcagc aagcgaaacg agaaaggatt
    21661 aaaaagccgg acttcaatgc ggggatggct gtcaagcaag cgtaaaatgt cgtcaagtcc
    21721 gggcgtattg ttgtcgtcca gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa
    21781 cagggcggaa aacagtaacc gacccgacat atcgtcctgc cagatgtaat actgaacatc
    21841 cagcgtatgc tctgccattt cggccagacg ataccgggcg gcaaacgcat ccagactttt
    21901 ctccagaggg aaaagaccac actgaccggg atgttgcgaa cacagtggca gcaccgcgct
    21961 cgccagccgg ggcaaatcat tcatctcctt gttgggtaag gagtctttcg tagaggtggg
    22021 cgttttcttc atcataacag acaaagtata cctgttcggg taaagcgtga cgggtaataa
    22081 attctgaaac ggttttaact gcgatttcag ccgctgccgc acgagggtaa ccataaaccc
    22141 cagtactgat tgcaggaaaa gccactgacg tatagctgtt tgccgccacc agtcgtaggc
    22201 tattgagata ggcatcctgc aaaagctggt cttcgttttg ttcaccacca cgccagactg
    22261 gcccgacggt gtgcactacg gctttagcgg gaagagcgcc tgcaagcgta ataacggcat
    22321 gccccgtagg gcaatcgccc tgctgttgcc tgacttttaa acaggcatcc agcagggccg
    22381 gacccgctga gcgatgaatg gccccatcga cgccgccgcc tcccattaat gacggattag
    22441 ccgcattcac aatcacatca acggccagtt tggtaatatc accctgcaca acatgaatac
    22501 gcgttttcat aacgcctcct tacaccttct tgtttcttaa gtgtacagca ggctttttgg
    22561 ttaaatcgca tttttctgct cagcaacgtg cgttgttcag aaacttagtt atattcgatg
    22621 ttcattacag ccagcttttt ctgtttatca agatactgca cttttactga ggctatttgc
    22681 gcgtttttaa cattaccgct cattaacgct ttgctggaat aggttttgcc cggaacagaa
    22741 ccattctgtg ggcagttcat ttctacgggt gatgactggg tgctgacgtc acatgccggt
    22801 tccacaattt ggccataaaa atggatcccc ccccttgcgc ggtgcttcct gcgatggcaa
    22861 taaaaggaaa aagcaaactg ccgagcataa gagagtttaa aaaaggtctg aacatttatt
    22921 ctatttcctc aatgattagt catccttgag ggttgtgtta tccatacttt caggttggct
    22981 gattcgcagc agaccattct ctccagattc atcttatgct cgatatttca acaaattaag
    23041 acttttctga agagggcggc cattgttgtg ataaatgaag tgactgtcca tcagaaacag
    23101 taacaactat tttcacccga tcttccgggg aaatatttaa actcaacttc gtcaaagcaa
    23161 taggttgatt agcaggcaat gagagggtct tttcttgctt cgtctgactt tgccctgaac
    23221 tgccttcgcg cagggacaat atttgtactc tgcacagaca agattgagta agagtgactt
    23281 caggaataat ggtatacaca tccccttgct gggtcgtatt aaaggttatc tggctggaaa
    23341 gtgccgcaag gagtaataac gcattcatat tcttctcccg aaaaaaaaca gggcttgcgc
    23401 cctgtttctg taatacaaat gatgtattag tactgatgag cggtcgcgtt gttaccaaag
    23461 ccaacctgag tcacgttgac ggaggagtta gatgcagtct ggtcaactgc agcaccgttg
    23521 ccaccaccga actgtttaac agtcatttca gaatttttgc cgttccactg atcaagagtg
    23581 gcgctgttac cgaagccacg ttgggtcaga tcgattgagc tgtcatctga gccctgacct
    23641 acatctgcac cattaccacc gccatgctgg gtaatagtca agtcagagtt acgggcatca
    23701 gtttgcagag caagtgcaga gttaccgcca ccgtactggt aaatgttcag ctctgaattt
    23761 gggccgctat tattaccgcc accaccgtgg ttaccgccgc cgccgtactg aggaacaaca
    23821 cctgccagag cgctaccgga gaatacgatt gctgcaattg ctgctacttt taaaagtttc
    23881 atgtaaaacc cccatcggat tgatttaaaa gtcgaatgga aattaacgtt gtgtcacgcg
    23941 aatagccatt tgcgactgtc tctgcactac aattgccgtt ttttgagtac catactgtgt
    24001 aatatttgct ttattaccag aacctttctg gataatcatc gcagtattac cataagcacc
    24061 ttgcgaaata ctggcatcgt tggcactgcc cgcctgatca atatatgcaa ggttataatc
    24121 tcctgtctgg tcaatctttg cccggttgct actaccttct tgcgcaacaa ccgccaaaag
    24181 ttttgagcct ccctgccgta actgagcact attattcgtc ccagcttgac caattatggc
    24241 tgcctgatta aatgaagact tactcaattc atttaccgcg aagttatatt ctgaattagc
    24301 taaatcataa cctgctgcgg ctgcaatccc aggcgcaccc agtattgtta acatcataaa
    24361 taacaatttg tttttcatgt tgtcaccctg gacctggtcg tacatttaag aaattaaatc
    24421 atttcaactt ggttgttaac gcaacctgta ttttgttaac gctgcgttac gatggaaagt
    24481 atgtctgcgg aaatattttt aataactcac ccgcgcgttg tatttttctt tttttgttca
    24541 taccaaagta ttaaaaccca cgtaaatacg ctgttatcta cgcaaaaaat attttgtttt
    24601 cttttaaatc tccgttttcc gctatcaaaa atcaccagac agtcattttt cttgcccgtc
    24661 gctgattgct gcgatatgtc ttgcgcacaa gccgtgacaa acctcgtctt tttgcagtaa
    24721 aaaattgtcc acggaggtgc ggagaaaaaa caagaacgtt ttacatgacg aaaggactac
    24781 accgaaatat tttttatatg tattattagt aagttatcac catttgtatg attttttaaa
    24841 attgtgcaat aaaaaccaaa tgtacaactt ttctatcatt tctaaactta ataaaacctt
    24901 aagattaaca ttttaatata acgagttaca tttagttaca tgtttaacac ttgatttaag
    24961 atttgtaatg gctagattga aatcagatgt aatccattag ttttatattt tacccattta
    25021 gggctgattt attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat
    25081 tttagaggca gctgtcaggt gtgcgatcaa taaaaaaagc ggggtttcat catgtttaat
    25141 gaagtccata gtattcatgg tcatacatta ttgttgatca ctaaaccgtc tttgcaagcg
    25201 acagctctct tgcagcacct taaacaatcg ctggcaatta caggaaaatt acataatatt
    25261 caacgttctc tggacgatat atcttcaggc tctattattc ttctggatat gatggaagca
    25321 gataaaaagc ttatccatta ttggcaggat actttgagca ggaaaaacaa caatatcaaa
    25381 atattgttgc taaatacgcc tgaagattac ccgtaccgcg acattgaaaa ctggcctcat
    25441 atcaacggcg ttttttatgc catggaggat caagaacgtg ttgtcaatgg gttgcaaggc
    25501 gtcctgcgcg gcgaatgcta ctttacgcaa aagcttgcca gttacctgat tacacattct
    25561 ggtaactatc gttataacag cacggaatca gccctcctta ctcatcggga aaaagagatc
    25621 ctgaataagc tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc
    25681 gaaaatacgg ttaaaacgca tctttataat cttttcaaga agatagccgt aaaaaaccgg
    25741 acacaagcgg tttcctgggc aaacgataac ctcaggcgat aaagccatga aacgttattt
    25801 acgctggatt gtggcggcag aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt
    25861 agaagtcccg ggattgttaa ctgaccatac tgtttcatct attggccatg atttttaccg
    25921 agcctttagt gataaatggg aaagtgacta tacgggtaac ttaacgatta atgaaaggcc
    25981 cagtgcacga tggggaagct ggatcactat aacggtcaat caggacgtta ttttccagac
    26041 ttttttattt ccgttgaaaa gagacttcga gaaaactgtc gtctttgcac tgattcaaac
    26101 tgaagaagca ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg gcgatttggc
    26161 gcatgatgaa ttctaaataa aaaattgttc ggaggctgca atgcgtgtca aacatgcagt
    26221 agttctactc atgcttattt cgccattaag ttgggctgga accatgactt tccagttccg
    26281 taatccaaac tttggtggta acccaaataa tggcgctttt ttattaaata gcgctcaggc
    26341 ccaaaactct tataaagatc cgagctataa cgatgacttt ggtattgaaa caccctcagc
    26401 gttagataac tttactcagg ccatccagtc acaaatttta ggtgggctac tgtcgaatat
    26461 taataccggt aaaccgggcc gcatggtgac caacgattat attgttgata ttgctaaccg
    26521 cgatggtcaa ttgcagttga acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca
    26581 ggtttcgggt ttacaaaata actcaaccga tttttaagcc ccagcttcat aaggaaaata
    26641 atcatgcagc gcttatttct tttggttgcc gtcatgttac tgagcggatg cttaaccgcc
    26701 ccgcctaaag aagccgccag accgacatta atgcctcgag ctcagagcta caaagatttg
    26761 acccatctgc cagcgccgac gggtaagatc tttgtttcgg tatacaacat tcaggacgaa
    26821 accgggcaat ttaaacccta cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc
    26881 acggcaatgc tggtcacggc attgaaagat tctcgctggt ttataccgct ggagcgccag
    26941 ggcttacaaa acctgcttaa cgagcgcaag attattcgtg cggcacaaga aaacggcacg
    27001 gttgccatta ataaccgaat cccgctgcaa tctttaacgg cggcaaatat tatggttgaa
    27061 ggttcgatta tcggttatga aagcaacgtt aaatcgggcg gtgttggggc aagatatttt
    27121 gggatcggtg cagacacgca ataccagctc gatcagattg ccgtgaacct gcgcgtcgtc
    27181 aatgtgagta ccggtgagat cctctcctcg gtgaacacca gtaagacgat actttcctat
    27241 gaagttcagg ccggggtttt ccgctttatt gactaccagc gcttgctgga aggggaagtg
    27301 ggttacacct caaacgaacc cgttatgctg tgtctgatgt cggctatcga aacaggggtc
    27361 attttcctga ttaatgatgg tatcgaccgt ggtctgtggg atttgcagaa caaagcagaa
    27421 cggcagaacg acattctggt gaaataccgc catatgtcgg ttccaccgga atcctgacag
    27481 ataaaaaagc cggaattatc atccggcatt ttactgttta agcgaaagcg tgagtgcccc
    27541 tcacgcttta tttttgattt cctgctcacg gtgagccgtc tttgcactac gcgccgccag
    27601 ccacaggccg ctgtttttca ttgcgtaacc aaataccagc ccaagcgcca gcgaaggtaa
    27661 caccagtttc cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa acgtccccgg
    27721 aacaaatgaa agcagcagct gtttggcctg aatacacatc agaaacgcga caataccggt
    27781 tatgacataa ccgagaattt ccagatgtgg tgccagtgca ctaccgtaaa taatgaccat
    27841 cgcccacacc acgccactaa gcagggttgc agcggagatc gccagccctt tcagcccacc
    27901 ttgcgggcag gcaaagtagg ccgtacagcc caggaagccc gcccagctta gtaagcccag
    27961 ggaaacagcc acccatcccc agataccgga gagaatgcct gttgtgattg caatagagag
    28021 aagtatgttc atgccgcgca tgatagcaga aaacgcggcc atgaatgaga tcgagcacac
    28081 attttatgca acaacaaatt atctgttgca gttatgagtg acgatgatca catttactct
    28141 tcagaatctt cttccagctc gtcccacatt gcactaatgg catcacgggt tagcggtgcc
    28201 atggtgcgcc agaaaggcgt ggttgcatgg gcctccactt tgccgaggaa cgcaccacac
    28261 cagggtaaca gatactcact gaacagtgtt tccagtgctt cgctctcatc ttccgttgac
    28321 tgatcttcca gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc tggcgtatcc
    28381 gccagtggca tccctcgttc ggaaagaaaa gcgcgcactt ccgcttccgt tgcatcctca
    28441 acccatgcgc tacgatatgg cggcacggca cattcatcgc cgataaacaa cgcattgtaa
    28501 tcggcagaga cttgcgccat atcacagctt ttctgtaaac gcgtcagtaa ctcatcctgc
    28561 tccagtggcc agttcgcagc cagtttcccc tcacgaatca gggtaaacag cggcaccagt
    28621 aaaggatctt gcggttggcg gtaatagagc gaacccagca cacgacagag gatagaaaac
    28681 tcgttcatta atatatttcc attactagta attaaaggtc tgcaaattcc gcaatcggtg
    28741 ccataccgcg agattcaagg aagttcagta agcggcgcgg agaaacattc aaaatgcgct
    28801 ctggcggaaa atctaccgcg tcgaggattt taagacactc ttcaaattcc cccatggtaa
    28861 acgcggtgtg agaatccgag cctaatgcca cccaacctcc agcatcacgt accgctgcgg
    28921 ctaccgcacg acagttgtct tcactgccct tacgtgagtg taaaaatgaa gaattattga
    28981 tttccagcgc cacctgatgt ttcgcggctg cttcagcaac ggctttcaca tctatttcat
    29041 atttgggatt tccgggatgg cttattatat gcacattgcc gctggcgata gtggcgatca
    29101 tcgcttgtgt attggtcgct ttgtcatgtg gtgcaaaaac cggctcatga aaaccggcaa
    29161 taatcagatc cagcgagtca aacattttac cgctgcaatc aatttcacca tcaacatttt
    29221 taatgttagc ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc ggccagatac
    29281 gcatgttaat gaagtgccag tgatgcggag catcttccat atccgggcca tgatcggtga
    29341 tcgcaaaaag tttaatgccc ttttgtttgg cctgggcaat gtaatcactc aatgtgctat
    29401 atgcatgtgt gctggcaacg gtatgcatat gaaggtcgac gggatacata actctctcct
    29461 gtattcaatt cccgccaagg atagcaggaa tcctgatgct ttattagtag ccgcgtgcgc
    29521 ggtcgacttg cccgcagacc ctctcccctt tttcgagctg ggcaatggtg cgagaaatgt
    29581 actccacagc ttcagcggga cgggtaatcg cggcgacatg tggtgttatc gtcacgcgtg
    29641 gatgttgcca gagcggactt tcaggcggta agggttcacg attaaaaaca tccaacattg
    29701 cgcctttaac tttgccgcta tccagcgccg cgagcaggtc atcttccaca acatgaacac
    29761 cacgcgccag gttgaggaga tacgcgccat ccggtaattt ttcgagtaat tgttgattaa
    29821 taatgccgac ggtttcaggg gtattcggta acaaattaat caatacccga cattggctca
    29881 gaaatgcaga cagttcttcc cgtccggcaa agctttgcac gccaggccac gatttacggg
    29941 ttcgactcca gcaacgcagc ggaaagcgcc aggtttgcag actctgagca actttactgc
    30001 ccagtacgcc tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat tcaggcagcg
    30061 gttgccaatg cgaactattt tgctggatgc gataatcgtc aaaacgtcga aaccaatgca
    30121 gcacctgact gacagcatat tcctgcattt gctcgcccat accggtatct tccaggcgaa
    30181 aaagtggaac agaagggttc agcatttcag ggtgtgcctg tagcttgctc aaaatagaat
    30241 caacaccggc cccgagtgcg aacaccgctt taagatcgcg ccctgccagc atttcaacag
    30301 gaggatgcca gactaaagca taatcagcag agtcattatc tccgcttttc catgctctga
    30361 ctcttgcctg aggaatagct ttgcgcagtg cctcaatcca ccattgggta tcgaacgttg
    30421 ggtgataaaa gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa tttattcgca
    30481 cttatcgtta tgatctacaa aggccaccag cataacaaat ccgtggtcgg tggcaaaaaa
    30541 agcagatttc gcttactaaa accacacatt gattgaagtt tgaataaacg cgcgattttt
    30601 tcaaaaagtt tgttgacctc aggtcatgat ttccctaaat tagcgcccgt tccagcaaga
    30661 caggaacgac aatttggtga ggtgtccgag tggctgaagg agcacgcctg gaaagtgtgt
    30721 atacggcaac gtatcggggg ttcgaatccc cccctcaccg ccatatttaa agaagagccc
    30781 gtacgaaagt acgggctttt ttttcgtata ttgcacacac cgggtgcccc cgcccacgtc
    30841 tgttagggcg aggggaaatt gtgcacatca actgtctggt tactcagtca acaatcaaaa
    30901 tgcgcaagat agtagagaac atatatgcgt tatctggtca cttcctgtaa cttcctcact
    30961 tattaaaatg cccccaacgg aaatacatgt cacatgccat cataaaatcg ttatgccaga
    31021 ggcatcgaca atggaaaaaa tagtaaatta agcacggtac atagcagagt aaaaaatagg
    31081 cttcaaacca aatccgccat tctacaagcg ttgccaatac cgacaccagc gtaatccaca
    31141 tcatttcaat aaccagcaat atgaataacc gaagccaaag cggcttggca aaaaaggtcg
    31201 acaacgcatt ttcactggag aaaaacagat cgatttcctg caaaaaaagc ctgcccagaa
    31261 aagcgtaccg cttggagaag cgcagaaaaa acagattaat cgccaacact acgccacaaa
    31321 gaatgagcaa tgcaattatc gatttaaacg ttaaccactg cccctctgtc acaaaagaat
    31381 cactgttacg taacgggctg atagtaagca ggttaacaac cgttgagatg cacaccgaaa
    31441 gcagcacata gcgagtcaga cttttatatt ttactttttt cgctactgaa atagcgacct
    31501 gcttgacata actggtgcgg atcatccgaa acagcagaaa gatgaccaac ggcccaagta
    31561 acgaaatcag gaacagcgca tataacgagg gtagaagctg gtatagcccc caactcattg
    31621 cactcaacat gattaacgac agaaatcctg ccaccagagg ttgtgtaaga aataattcta
    31681 ttttgtggtt ctgctttttc ttaaaccacg aaaaatcgat attcttcgtg acgcatttat
    31741 aagcccagta cgccttgaga tcaaaaataa aattatacag caccagggcc agaatcccac
    31801 taacgacacc aatatcctct gatggaaaag taaatcctgc atttttccaa taaaagccta
    31861 tcagaataag tccaatagca taaatgtata tcagatttcg tttatcctga aagcgaataa
    31921 aagttaaata atcaggaatc atttgaatac ctttgcatca ctaaatgctt tacgacaatg
    31981 cataactcgt tgctgatcca cgccagcaaa atcatttaac atattcccca aatagggctt
    32041 attacttatt tcaaggaaag ctaccggggt tggcatattc agaaatgtat cgagagattc
    32101 agtccatttc accgtatggg ttaagtgtaa tgagagattt tctttgataa ttgacggagc
    32161 gacctcgctt tttgctgtta cgttcatcag aacctgatgc tctggcgaag cgatatccag
    32221 tccggcaaga taatcgcgca ttgcctgaac gccgtcttcc atcaaacgcg tgtgccaggc
    32281 a
//
LOCUS       DAFATA010000045        31684 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFATA010000045 DAFATA010000000
VERSION     DAFATA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.046"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(22..1135)
                     /gene="yneK"
                     /locus_tag="LGJ20_002530"
                     /pseudo
     CDS             complement(22..1135)
                     /gene="yneK"
                     /locus_tag="LGJ20_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(1213..2094)
                     /locus_tag="LGJ20_002531"
     CDS             complement(1213..2094)
                     /locus_tag="LGJ20_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2386578.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            2195..3583
                     /gene="sad"
                     /locus_tag="LGJ20_002532"
     CDS             2195..3583
                     /gene="sad"
                     /locus_tag="LGJ20_002532"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBK2386579.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            3647..4573
                     /gene="glsB"
                     /locus_tag="LGJ20_002533"
     CDS             3647..4573
                     /gene="glsB"
                     /locus_tag="LGJ20_002533"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBK2386580.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            4573..4932
                     /locus_tag="LGJ20_002534"
     CDS             4573..4932
                     /locus_tag="LGJ20_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBK2386581.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            5071..6489
                     /locus_tag="LGJ20_002535"
     CDS             5071..6489
                     /locus_tag="LGJ20_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2386582.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            6716..8167
                     /gene="uxaB"
                     /locus_tag="LGJ20_002536"
     CDS             6716..8167
                     /gene="uxaB"
                     /locus_tag="LGJ20_002536"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK2386583.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            8374..9288
                     /locus_tag="LGJ20_002537"
     CDS             8374..9288
                     /locus_tag="LGJ20_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBK2386584.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(9292..10050)
                     /gene="tam"
                     /locus_tag="LGJ20_002538"
     CDS             complement(9292..10050)
                     /gene="tam"
                     /locus_tag="LGJ20_002538"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBK2386585.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(10107..10397)
                     /gene="lsrG"
                     /locus_tag="LGJ20_002539"
     CDS             complement(10107..10397)
                     /gene="lsrG"
                     /locus_tag="LGJ20_002539"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBK2386586.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            complement(10421..11296)
                     /gene="lsrF"
                     /locus_tag="LGJ20_002540"
     CDS             complement(10421..11296)
                     /gene="lsrF"
                     /locus_tag="LGJ20_002540"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBK2386587.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            complement(11323..12345)
                     /gene="lsrB"
                     /locus_tag="LGJ20_002541"
     CDS             complement(11323..12345)
                     /gene="lsrB"
                     /locus_tag="LGJ20_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HBK2386588.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            complement(12357..13349)
                     /gene="lsrD"
                     /locus_tag="LGJ20_002542"
     CDS             complement(12357..13349)
                     /gene="lsrD"
                     /locus_tag="LGJ20_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBK2386589.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            complement(13349..14377)
                     /gene="lsrC"
                     /locus_tag="LGJ20_002543"
     CDS             complement(13349..14377)
                     /gene="lsrC"
                     /locus_tag="LGJ20_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBK2386590.1"
                     /translation="MLKFIQNNREITALLAVVLLFVFPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKELSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKNREAA"
     gene            complement(14371..15906)
                     /gene="lsrA"
                     /locus_tag="LGJ20_002544"
     CDS             complement(14371..15906)
                     /gene="lsrA"
                     /locus_tag="LGJ20_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBK2386591.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            16155..17108
                     /gene="lsrR"
                     /locus_tag="LGJ20_002545"
     CDS             16155..17108
                     /gene="lsrR"
                     /locus_tag="LGJ20_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBK2386592.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            17187..18779
                     /gene="lsrK"
                     /locus_tag="LGJ20_002546"
     CDS             17187..18779
                     /gene="lsrK"
                     /locus_tag="LGJ20_002546"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HBK2386593.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            19310..24730
                     /locus_tag="LGJ20_002547"
     CDS             19310..24730
                     /locus_tag="LGJ20_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HBK2386594.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMDISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDMTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGHEGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            24939..25205
                     /gene="hipB"
                     /locus_tag="LGJ20_002548"
     CDS             24939..25205
                     /gene="hipB"
                     /locus_tag="LGJ20_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBK2386595.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            25205..26527
                     /gene="hipA"
                     /locus_tag="LGJ20_002549"
     CDS             25205..26527
                     /gene="hipA"
                     /locus_tag="LGJ20_002549"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HBK2386596.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            26839..27047
                     /locus_tag="LGJ20_002550"
                     /pseudo
     CDS             26839..27047
                     /locus_tag="LGJ20_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            27481..28044
                     /gene="fimA"
                     /locus_tag="LGJ20_002551"
     CDS             27481..28044
                     /gene="fimA"
                     /locus_tag="LGJ20_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBK2386597.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSTAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            28405..29115
                     /locus_tag="LGJ20_002552"
     CDS             28405..29115
                     /locus_tag="LGJ20_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBK2386598.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            29157..>31684
                     /locus_tag="LGJ20_002553"
     CDS             29157..>31684
                     /locus_tag="LGJ20_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2386599.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLP"
BASE COUNT         8229 a         7346 c         8033 g         8076 t
ORIGIN      
        1 gagagtcggt aacatcacgg tttaatgacg ttcagggagg tctggcgttt tggcgattgc
       61 gggtataggt ggtctgacag gggagcgagg cctgtttttc accaggtctt cgtgagtaac
      121 ttttggcggc gtttcatttt tacaaacagt ttgagggtcc tgcgcgccat atgtttgata
      181 attttatcat cacttaatga tgctggcatc aacacgtcat tcagatcact ttcattggga
      241 ttcataaatt ggtgatagtg caataacata ttggcgaaaa ataaaaaaaa gtcctgaaaa
      301 tgaggagatt cttgttctgc taaaagggat aaaaagcaga tgcagacttc gggtagtaca
      361 tgtttgttag aacctaatga atccatcagg ctttgcaata aatcctcttt taattcgctg
      421 tccgttatgt cgagaatggc ggtgttcaag ttaggatctt tattgacgaa ccatctgtga
      481 acctcagagt tattccataa tcgggggagt atgacaaaat taccgcgaga gataaaattc
      541 aggacaagca aatcaaactg gcgtaagtaa tctgcagcgg tatggtgatc cggaggcagg
      601 ggttcaatat gtggtgtatg cgctcttttg atgggaacgc caaggttgat cattggaaat
      661 tgttcaataa aatcattcag aaacagaaat agcacacgct cgacgccggt ctgaaatacg
      721 cgccccatag tgacaggctt tgggttggga gcgcaaaaat attaatttct ccatcatggc
      781 gaaaattatc taaataaata gggcttaaat ttattactac ctgaaattca atattttctt
      841 tgtgtcttcc attcttgacg ggataagtga gtttaaactg aaccttttca ttgtatagcg
      901 tgtcgttacc ccgaaaagaa gtcctctgaa tatatgataa gttatcaagg ttattaaata
      961 tcttttttgt ggctgagttg atgtttaaag acgtgcgcca ctcttcatca catattattg
     1021 tacttagcgc atcaataaag tcctttaaaa cttcttttac ctgaggtgca atattacgta
     1081 cgggaaaatc atcaggaaga gatatgtaat tactgatgct tactggcgta accatatttg
     1141 ccccatatag tcataagtca tcggaacatc actatatagg gcatttgagc tgcaagaact
     1201 atcaaaaata gctcattggt aaccctgttt tgccgagtca ggcacatcca gtagttgaat
     1261 aaatgcctca agcggacgcg ttttcgcacc acgacgccag accagccagg ttgttaacca
     1321 acgccattgc tcagctaacg gccacgcttc aacctggtga tgccccggca tactttccag
     1381 catagagcgt ggaataagcg caatgcctgc tcctgcgacc acacaggcca acattccgtg
     1441 ataagactcc atctcatgga tagttcccgg agcggcaccg tcagcatgaa accagctctc
     1501 gaagtggcga cgatacgaac aattggcgcg gaaggcataa atgttactgc cgttaacctg
     1561 actggcgcgg attactggcg catgaccttg tggcgtgacg atcatcagct cctcgcggta
     1621 taccggtatc ccgtcaatgg cagtatggtt aatgggccca tcaataaatg ccgcattcag
     1681 ttttccctcc agcactcctt ccagcatagt gccggaaggg ccggtggaaa gtgaaaactg
     1741 aattttggga taacgatggt tgaattccgc cagcgtggcc ggaatgcgca ctgctgcggt
     1801 gctttccagc gaaccaagag aaaataaacc ttgcggctca tcgccagcga caacgctccg
     1861 cgcttcatcc actaacgcga gaatttgttg gctatagcgt aaaaagttat gcccggcagg
     1921 agagagacgt aaacgctgat tctcgcgaat aaacagatca acccccagtt ctgtttccag
     1981 ctggcgtaga cgggtagtga gattcgacgg cacgcgatgc acttttgctg cagcctgggt
     2041 tatgctgcca gcttcggcaa ccgcgttgaa catctccagt tgggtcagat ccataatatt
     2101 ctcgattcgt gaataagtgg cttaatatta ttcattttaa agcaagagta aatctgcgta
     2161 tcttcatacc atgactcata aaggagatac cccgatgacc attactccgg caactcatgc
     2221 aatttcaata aatcctgcca cgggtgaaca actttctgtg ctgccgtggg ctggcgctaa
     2281 cgatatcgaa aacgcacttc agctggcggc agcaggcttt cgcgactggc gcgagacaaa
     2341 tatagattat cgtgctgaaa aactgcgtga tatcggtaag gctctgcgcg ctcgtagcga
     2401 agaaatggcg caaatgatca cccgcgaaat gggcaaacca atcaaccagg cgcgcgctga
     2461 agtggcgaaa tcggcgaatt tgtgtgactg gtatgcagaa catggtccgg caatgctgaa
     2521 ggcggaacct acgctggtgg aaaatcagca ggcggttatt gagtatcgac cgttggggac
     2581 gattctggcg attatgccgt ggaattttcc gttatggcag gtgatgcgtg gcgcggttcc
     2641 catcattctt gcaggtaacg gctacttact taaacatgcg ccgaatgtga tgggctgtgc
     2701 tcagctcatt gcccaggtgt ttaaagatgc gggaatcccg caaggcgtat atggctggct
     2761 gaatgccgac aacgacggtg tcagtcaaat gattaaagat tcgcgcattg ctgctgtcac
     2821 ggtgaccgga agtgttcgtg cgggagcggc tattggcgca caggctggag cggcactgaa
     2881 aaaatgcgta ctggaactgg gcggttcgga tccatttatt gtgcttaacg atgccgatct
     2941 ggaactggcg gttaaagcgg cggtagccgg acgttatcag aataccggac aggtttgtgc
     3001 agcggcaaaa cgctttatta tcgaagaggg aattgcttct gcatttaccg aacgttttgt
     3061 ggcagctgcg gcagccttga aaatgggcga tccccgtgat gaagagaacg ctctcggacc
     3121 aatggctcgt tttgatttac gtgatgagct gcatcatcag gtggagaaaa ccctggcgca
     3181 gggtgcgcgt ttgttactgg gcggggaaaa gatggctggg gcaggtaact actatccgcc
     3241 aacggttctg gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc
     3301 tgttgcggca atcaccgttg cgaaagatgc agaacatgcg ctggaactgg ctaatgatag
     3361 tgagttcggc ctttcagcga ccatttttac caccgacgaa acacaggcca gacagatggc
     3421 ggcacgtctg gaatgcggtg gggtgtttat caatggttat tgtgccagcg acgcgcgagt
     3481 ggcctttggt ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt tcggcttaca
     3541 cgaattctgt aatatccaga cggtgtggaa agaccggatc tgacgcgaca tattcagctc
     3601 tgatatactc gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg cagtcgccat
     3661 ggataatgca attttagaaa acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa
     3721 agtcgcggat tatattccgg cgctggctac agtagacggt tcccgattgg gcattgctat
     3781 ctgtaccgtt gacggacagc tttttcaggc cggagacgcg caagaacgtt tttccattca
     3841 gtctatttcc aaagtgctga gtctcgttgt cgccatgcgt cattactccg aagaggaaat
     3901 ctggcaacgc gtcggcaaag atccgtctgg atcaccgttc aattctttag tgcaactgga
     3961 aatggagcag ggtattccgc gtaatccgtt tattaatgct ggtgcgctgg tggtttgcga
     4021 tatgttgcaa gggcgattaa gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt
     4081 aagcggtgtg tctgatattt catacgatac ggtggtagcg cgttccgaat ttgaacattc
     4141 cgcgcgaaat gcggctatcg cctggctgat gaagtcgttt ggcaatttcc atcatgatgt
     4201 gacaaccgtt ctgcaaaact actttcatta ctgcgctctg aaaatgagct gtgtagagct
     4261 ggcccggacg tttgtctttc tggctaatca ggggaaagct attcatattg atgaacctgt
     4321 ggtgacgcca atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca
     4381 gaacgcgggg gagtttgcct ggcgggtggg gctaccggcg aaatctggcg ttggtggtgg
     4441 tattgtggcg attgttccgc atgaaatggc catcgctgtc tggagtccgg aactggatga
     4501 tgcaggtaac tcgcttgcgg gtattgccgt ccttgaacaa ttgacgaaac agttagggcg
     4561 ttcggtttat taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt
     4621 cgctctcgct ttcgtctggg catgaaagag cgtcagtatt gcctggagaa aggcgcgcca
     4681 gtcatcgaac aacatgcggc ggattttgtc gctaaacgtc ttgctccggc attaccggct
     4741 aatgacggta agcaaacccc catgcgcggg catccggtgt ttatcgctca gcacgccacc
     4801 gcgacctgct gtcgcggttg tctggctaag tggcataaca ttccgcaggg tgtttcgtta
     4861 agcgaagaac aacaacgtta tatcgtggcg gtaatctatc actggctggt tgtacagatg
     4921 aatcagccat gagaattgcg gaaaacgata aaatagcatt cccagggtac ctttatttgc
     4981 cgccgctgtt agcgaacatg ctatccttta tgagaattta cttattacgt tgagataatt
     5041 gccacccgca attgtatgga cagataatta atgcatgtac accccatttc aactttccgt
     5101 ttgttccagg aaggtcatct gctacgtaat agcatcgcta tttttgcgct aaccacgctg
     5161 ttctatttta ttggtgcaga gttacggctg gttcacgaac tttctctttt ctggccgctg
     5221 aatggcgtaa tggcgggggt gtttgcccgc tatgtctggc ttaatcgact gcattactat
     5281 gcgatcagtt atgtggcgat gctggtttat gatgccataa ccaccgaatg gggactggtt
     5341 tcactggtta tcaatttctc caatatgatg tttattgtta ccgtcgcctt actggtcgtg
     5401 cgggataagc gtctggggaa aaataagtat gagccagtaa gtgccttacg gctatttaat
     5461 tactgtctca ttgccgcatt attatgcgct attgtcgggg cgattggttc ggtcagtatt
     5521 gatagtctgg atttctggcc tttgcttgcg gactggttca gtgagcaatt ctcaacgggc
     5581 gttttgatcg tgccttgtat gctgacgttg gctattcctg gagtgctgcc gcgctttaaa
     5641 gcagaacaga tgatgcccgc catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt
     5701 ggcggagcgg ggagtctggc gttcccgctg cctgcattaa tctggtgtgc agtgcgctat
     5761 acgccgcagg taacatgtct gttgaccttt gtcaccggtg cggtggaaat cgtactggtg
     5821 gcaaattcgg tgattgatat ctcggtcggt tcgccgttct ccattccgga aatgttctcc
     5881 gcacgtctcg gtattgccac gatggcgata tgcccaatta tggtttcttt tagcgtggca
     5941 gcgatcaatt cgctaatgaa gcaagttgcg ctgcgagccg actttgattt tctgactcag
     6001 gtttactcac ggtccggtct ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa
     6061 catctgacgg tcatgctgct tgatattgac tatttcaaaa gcattaacga taactatgga
     6121 catgaatgtg gcgataaagt gttaagcgtg tttgcccggc atattcagaa gattgtcggt
     6181 gataaggggc tggtggcgcg catgggcggc gaagaatttg ctgttgcagt gccgtcggtg
     6241 aatcctgtcg atggtctgct aatggcggaa aaaatccgta aaggcgttga actgcaacca
     6301 ttcacctggc aacaaaaaac gctttatctc acggtaagta ttggcgtcgg tagtggtcgc
     6361 gcatcgtacc gaacgctgac cgatgatttt aataaattga tggtcgaagc cgatacgtgt
     6421 ctgtatcgct cgaagaaaga tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa
     6481 gttgtctgac atctttattc attccgcgcg atttgaatgc aatgtgttct accactttat
     6541 gatccaggaa aaggcgtatt tcctcgatta aaccatgatc cgcgccacac ttttctcata
     6601 aaactgctca ttaatcatca ggagttgatc aaaacagggg ttcggcgtat taaagtttgc
     6661 taacctgtcg taccaattca aacaggttgt atgactaatc agaagggaac ccattgtgaa
     6721 aacactaaat cgtcgcgatt ttcccggtgc acagtatcca gaacgtatca ttcagtttgg
     6781 tgaaggtaac ttcctgcgcg cctttgttga ctggcaaatc gatctcctga atgagcacac
     6841 cgatctgaat tctggcgtgg tcgttgttcg tccgattgaa acttcattcc cgccgtcact
     6901 gagcacgcag gatggtctgt acaccaccat tatccgtggc ctgaatgaga agggggaagc
     6961 ggtcagcgac gcgcgtctga ttcgctctgt taatcgtgaa atcagcgtct acagtgaata
     7021 cgatgaattc ctgaaactgg cgcacaatcc ggaaatgcgc tttgttttct ctaacaccac
     7081 cgaagcgggt atcagctacc atgcgggcga taaattcgat gacgcgccag cggtaagcta
     7141 tcccgcaaaa ctgacccgtc tgctgttcga acgttttagc catttcaacg gtgcgctgga
     7201 taaaggttgg atcatcatcc cgtgtgagtt gatcgactac aatggcgacg cgttgcgtga
     7261 actggtgctg cgctatgctc aagagtgggc actgccagaa gcatttattc aatggttgga
     7321 tcaggctaac agcttctgtt ctacgctggt ggaccgtatc gttaccggtt atccgcgcga
     7381 tgaagtggct aaactggaag aagaactggg ttatcacgat ggtttcctcg acaccgctga
     7441 acacttttac ctgtttgtga ttcaggggcc gaaatcctta gcgactgaac tgcgtctgga
     7501 caaatatccg ctcaacgtgc tgattgttga cgatattaaa ccgtacaaag aacgcaaagt
     7561 ggcaatcctc aacggtgcgc acaccgcgct ggtgccggtg gcgttccagg cagggctgga
     7621 taccgtaggt gaagcgatga acgatgctga aatttgcgca ttcgttgaaa aagctatcta
     7681 cgaagaaatt attccggtac tggatttgcc tcgtgatgaa ctggaatctt tcgccagtgc
     7741 agtcaccggg cgtttccgta acccgtacat taagcatcag ctgctgtcta tcgcgctcaa
     7801 cggtatgacc aagttccgca ctcgcatcct gccgcagctg ctggcagggc agaaggcaaa
     7861 aggcacactt ccggcgcgcc ttactttcgc attagcggca ttgattgcct tctatcgtgg
     7921 tgagcgtaac ggagaaacat atccggtaca ggacgatgca cactggctgg aacgttatca
     7981 gcaactctgg agccagcatc gtgaccgtgt tatcggtacc caggaactgg tagcgattgt
     8041 gttggcagaa aaagaccact gggagcagga cctgacgcaa gtgcctggtc tggtcgagca
     8101 ggttgctaat gacctggatg cgattcttga aaaagggatg cgcgaagctg tacgtccgtt
     8161 gtgctaataa caccgttact tctgaacccg gtttcgaccg ggtttcttat ttcactatat
     8221 tagttacaac atcctatgtg cctttatcat taacagatgc cacatgcagc gcctgttaat
     8281 gttgtaagct tgtaggtaat ttgtaagtag cgtcttgaga atgtgatcgt aatcacgttt
     8341 aatagggcga ctttttcacg cctgaagata accatgattg ttcgtccaca acaacactgg
     8401 ctgcgccgta tttttgtctg gcacggctca gtattatcca agatatcatc gcgcttacta
     8461 ctcaattttc tcttttctat cgctgttatt ttcatgctgc cctggtacac gcatctgggc
     8521 atcaaattca ccctcgcacc gttcagcatt ctcggtgtcg ccatcgccat ttttcttggt
     8581 tttcgtaata atgccgggta cgcccgttac gttgaagcgc gaaaactttg ggggcagttg
     8641 atgattgcct cacggtcgtt actgcgcgag gtaaaaacga cattgccgga ttcggcaagt
     8701 gtaagggagt ttgcccggtt gcaaatcgcc ttcgcccact gtttacgcat gacattacgc
     8761 aaacagccac aggcggaagt gctggctcat tatctcaaga ctgaagatct tcagcctgta
     8821 ctggcttcga actctccagc taaccgtatc ttgttaataa tgggggagtg gttggcggtt
     8881 cagcgccgca atgggcagct ttcagatatc ctgtttatta gcctcaacga tcggcttaat
     8941 gatatttcag cggtcctggc aggatgcgag cgcattgcct atacgccaat tccctttgcc
     9001 tacaccctga ttttgcatcg taccgtttat ctgttttgta tcatgctgcc gttcgcgctg
     9061 gtcgtggacc tgcattacat gacgcctttt atctctgtgc tgatttccta cacttttatt
     9121 tcgctggatt gtctggcgga agaactggaa gatccgttcg gtactgaaaa caatgattta
     9181 ccgctggatg ccatctgcaa cgctattgaa attgacctgt tacagatgaa cgatgaagcc
     9241 gaaattccag caaaaattct tcccgatcgt cattaccagc tgacgtgata tttactccat
     9301 acgccgggca acaataaaaa gacgcggaaa tgccagcagt atctgtccgt tctcttgcag
     9361 tggatactgc tcttccagca tctgatggta gcgcttaaga aaaagctgct gttcgctctc
     9421 ggtcagatcc tgtaaccacg gacgtaatcc agtggcggtc acccaatcga taatcgcctg
     9481 gtgtgacggc atctggtgat agtaggtggt tcgccagata tcgacctcac atccggcttc
     9541 gctcaaaata tcgtagtaag catgaacgcc agccaacggc tcacgcccgc gatccgggta
     9601 gttttgttcc caggcaactt cgcgcatgag cacatgggtc ggctccagcc agttatctgg
     9661 catctgtact gccagcacgc cctgcggatt aagtaaagaa accagatgag gaaacaattc
     9721 gtagtggtcg ggcagccatt gcagtgaggc attagcaaaa atcagatcga gtgcctgtac
     9781 cggttgccag ttgcggatgt ctgcttccac gaactggcag tctggcaaag cactgcgcgc
     9841 ttcagcaatc atcgccggag acgagtcgat gcctgttatc ctggcagcag gccaacgttg
     9901 gtttagaagg gcggtgctgt tacctgggcc acagccaaga tcggcaacgt attcgatatt
     9961 ctccagcggt actctggcaa gcagctccac cgccggacgc gatcgttcag cggcaaagtg
    10021 cagatataaa gaggggttcc agtcagacat ttatttactc ccgtcagcgc gttaccccaa
    10081 ttgtagataa aactgatgaa ttcgcctcac ggcatcaacc cattgaacag acgtttttta
    10141 cgcggcccgg tcattaaaga ttccagtttc gcgacacagg ttttgtagtg gggcgtggtt
    10201 ttatggaatg ccactgcgtc ttcatcttta taggcttcgt agatataaaa gcgcgaattc
    10261 acttctgggt cctgtaagac atcgaagcgc aaattgcctt cttcctgtac agagcccagg
    10321 tggttctggc gaaaaacttc gataaactcg tcaatcttgt cttcatgaac gttaatttca
    10381 accagtgtga cgtgcataat tcttctcctt aaatccgcag ttactgtttt tcactcagat
    10441 agagttcata tgcccgatca gccgtttcgt tatggtgaac caccgcctgt acggctttca
    10501 tcatcgccac cggatggtca gactggaaaa tattacgccc catatccaca ccagaagcgc
    10561 cctgatcgat agcctgccag cacatttcca gcgcctcgcg ctccggtaat tttttaccgc
    10621 cagcaataac aatgggtacc ggacatccgg caacaatccg ttcaaaacct ttttcgacat
    10681 aataggtttt gataatttgc gcccccattt cagcggcgat tcgagtcgcg agcgagaaat
    10741 aacgctgatc gcgcaccata tctttgccca cgccagtcac ggccatggtc ggcattccca
    10801 ctttcattcc ggcatcgacc agctgaataa tatttttgat cgactgatgt tcatattcgc
    10861 tgccgatata aacctgcgcc gccaccgcgc aactgttcag gcgcacggcg tcatccatcg
    10921 ataacgccac ggcttcatta cttaattccg ccagaataga gttcgcacct gacgcccgca
    10981 gtaccaccgg cttattggtc gctgggggaa ctacgctgcg caaaatgccg cgcgtacaca
    11041 ttaatacatc ggcatgttca aacagcgggg cgatatttat atcaatgcgt tcaagtccgg
    11101 tagtcggccc ctgaaaataa ccatggtcaa aggccagcat cacggtttta cccgttttcg
    11161 gattaaatat ccgcgataag cgtgactgca ttccccaatc cagcgcaccg caacctttta
    11221 ggataaaagg gatattttgt tgcggttgat cggtacgaaa atctttacca tctttaatat
    11281 cgtctaaatc tgccataact tactccggtt aagtaatgca catcagaaat cgtatttgcc
    11341 gatattctct ttgttgaata tcacgcgctc cggtaacagt acgatgccat taccatccgc
    11401 ttcgtagtca tagccctgaa cgctgtttgg cgagacttcc acctgaccta cgccctggat
    11461 atccagcttg tcgcccgttt tcattgatcc ttttttcaat aatgcatccg cgacatacac
    11521 tgaaattttg ccttgctgaa ccacatccca caggccaaat tctttcaccg tgccgcgttc
    11581 cacatatgga cgcatcacgt ttggcgtact gaatccgaca atcgcaactt tgtcattttt
    11641 caggttttct gcggcttgtg cggcagcggg cagggcgttg gcgtcggggg cgataatggc
    11701 gtcgagatcg ctatacgctt ttaatattcc ttctgcggtt tgtaatgatt tagtggcatc
    11761 gttatagcca aactgcgtag tgacaatttc ccagccagga tgctctttgg cgattttcgc
    11821 tttcgcttct ttcacccact ggttttggtc cgtaacggtg gggcttgagt agaaaaacgc
    11881 gactttggct ttgtctttat tcacctgacg cgccgccata tccaccaaca tacctcccaa
    11941 ctgggcgggc gttccctgat taatgtagta agagcggcac tccggtttag tatcagagtc
    12001 ccaggtcagc actctcacac cacgttgcat ggcgcgtttc agtgccggac acaagccatc
    12061 aggcgaaacc gcagaaacga taatggcgtt ataaccttga ttgacgaagt tattaatcaa
    12121 ctgtacctga ccagaaacac tgggttctgt cggcccgtcg taggtcacat caacgctcag
    12181 ctctttaccc gcttgttgtg cgccgttgcc accgctggta aaaaatccca cgccaaccag
    12241 tttgggaata aatgcaatac gctctgcggc ctgcacattc atagagattg cggcaatgcc
    12301 aagagcgcta agtaaggcga ttttcttaaa gcgatgaagt gtcatgaaga tatcctttat
    12361 ggcaatgggt tattggcccg acgcgccagc cactctttaa tttgctggcg atgcaggcta
    12421 acggaacgac ctacgacaac gacgataagt agcgcaccgg aaagggcgct ggacacctga
    12481 tttggcactc ctgccatttg caaaccttgt tgcaaatatc ccactaataa aaccgcaatg
    12541 gcggtgccga taatggaacc ggaaccacca taaatattgg caccgccaag caccacggcg
    12601 gtgatggcgg gcatcagaaa cgacgcaccg agatcggaac gtgctgaacc aaaatacgac
    12661 accagcagca cagcggcgac cgcagacgcc agccccgtca tggcatagag cgcacataag
    12721 gtgcggttaa ctggaatcgc gctataaagc gccacgcgcg ggctttgccc aatcaaaaac
    12781 acattacgtc cggcatgggt tttatgcagc cagagccaga aaacgaggag acatatcagg
    12841 aagataatca gcggaacggg gagtcccagc acatccaggt tagcgaaatc tgtaaacgcc
    12901 atcgggaatc caccaatacc ttcgtacccc gtcgctccgg ccataccgga aagcagcaga
    12961 gcgcttccgg caaacagata cagcgtgcca agcgtaatca ccagcgggtt aactttggta
    13021 tagataatta atccggcgtt gatcagcccg cacaatgcgc cgagcagtaa ggtcaggagt
    13081 atcgccagcg gcatcggcac accactttga aacagtacgc ccaatgcaat ggcgcagagg
    13141 ccgatggtcg aaccaaacga aatatcgatc ccgccactga caatcaccat cgtcagcggt
    13201 agggcgacaa tgccaatgca gataaagtca ctggtgctga acagcaacat attgagatct
    13261 aacatccgcg ggttaattgc accaaatgcg acaatctcaa taacgagcag tgcggcaaga
    13321 gccagttccc aaccgtagcg aatacgcatt atgcggcctc ccgattttta cctgacgaag
    13381 cgggtttaac ggatggcggt ggcgtcataa agcgggcata tttttgccgc cgtagattac
    13441 gttccagcgc acaacgcagg cgtccatcaa acaccagcac cgccagcagc acgaggcccg
    13501 cgataaaatc gttccaccat gccggaatgc gcaacagcac cagtacgcta tcgatctgcg
    13561 tcaggaacca tgcgccgagt accgcaccaa tgatcgcacc ggaaccgccg agcaaactaa
    13621 tgccgcccag cacgcaggct gcaattgctt tcatctccag cccggtaccg gtctggttgg
    13681 ggataaaacc aatctgcgaa gcaaacacaa ttcccgccag tgccgccatg cagccgttca
    13741 acgaaaatgc cacaatgcga atggcttccg tacgaacacc cagttgacga gcgccctgta
    13801 aattatcgcc cgtggcataa aaactgcgtc caaacgccgt ctttgccagc agccaggcca
    13861 taaatgccac cagaattatc gtcaaccaac caatcgcgga aacgccaaac agcagcggtg
    13921 cggagagctc tttcagttcg gcgggtaacc cttcaatcca tttgccgcca gtccacagca
    13981 acatgatgcc tctgtacaac cctaacgtgc caagggtggc aacaatggca gggatcttta
    14041 gccacgcgac caggacaccg ttgaaaaatc ccgcgagcaa accaagcagt aaagtcgcga
    14101 cacaggcaac aggtagtgaa tatcctgcgt tcagtaacat ccccaacagc accgcgcaca
    14161 ttccggtaat cgaacccact gaaacatcaa tattgcgcgt aagcattacc agcgtcgcgc
    14221 ccattgccag taggatcagg atttgcgcgc tgctataaac catggtcagc gtttgcacac
    14281 ttaaatactg gcggtcgaga aaaccgggga atacaaacag cagcaccacc gccagcaatg
    14341 ccgtgatttc acggttgttc tgaataaact tcagcatgat gcctcctgac gctgactatc
    14401 gccgaaggca acgcgcataa tagtctcgac attaatatcg cgcccggtca gtgcagagtg
    14461 ggtaatttcg ccctgatgca tcacatacac acgatctgcc atcagttcga tctcttccag
    14521 atcagaggag ataagcagca cagccacatt ctgtgcggcg atgctgcgca acagctggta
    14581 gatatcatta cgtgccgaga catccacgcc gcgcgtcggc tcatcgacaa tcaatacttg
    14641 cggcgaagct tccaggcatt tggcaatgag gattttttgc tggttgccac cggataatgt
    14701 ccgtgcagct tgttccggtt ggttaaattt aatattcagc gcccgacgat aacgttccag
    14761 ggtggcatta tctttcgcgg ttttcgccca gaatccacga aggttatgag taagggcgca
    14821 gacgttccag gccagcgaag catcgagatt cagtccggat gactggcgat cttccggcag
    14881 ataaaccaga ccgcgcagta aacgttctcc agtggataat ttattgatct ctttaccatt
    14941 cagcataatg cgtccgccac gcaaagtacg cagaccatag agcgtctcgg ccagttctgt
    15001 gcgtccggct cccaccagcc cagccaggcc cagaatttct ccggcattga gcgtcaggct
    15061 gacattcctg aaaccttcgc cggtcagatt ttccagtgtc agcaccggcg ttccggcagc
    15121 atgttgtggg cggttaccag gtaactccag ccataatttt tggctggcag agagcgattt
    15181 ttcccgtacc gctggggtga tggcctgaat aatgtcgtcg gtagacagtt cgctggtttt
    15241 gccgcttaag gcgatggttc cgtcgcgcat cacgctaatt cgatcggcaa tctggcgaat
    15301 ttccggcagc ttatgcgaga taaaaacaat acccacgcca gtagcaagca gctcttgcaa
    15361 gcgactaaac aagcgttcgg tttccgcagg ggtaagcgag gcggtaggtt catcgaggat
    15421 cagaatccgc gagtcgcgca tcagcccgcg gaggatttcc accatttggc gatcggcgac
    15481 atccagcgat cctgccagac tatgcagatc aaactggcag cccagcgccg ccagcaagtt
    15541 cttcattttt tgcatggaga gctgtttttt tgccatccca aacaggatgt tttcttttat
    15601 cgacaggctt gggaaaagca gcggttcctg gggaacgaga taaatgccca gctgatgagc
    15661 atgaactggc gttaatctgg cgtagttgtt gcccccaatc tccagcgtac cgctatcagc
    15721 aggggtaata ccggcaataa tcttcattaa cgtcgattta ccggcaccat tgccgccgag
    15781 cagggcgtgg acctccccct gatgcaacgt aaaatcgatg cctttcagga cattgacccc
    15841 tgaatactgt ttataaaccg agcgggcgca aagtagcggt aacgcgcggg tatcactcgt
    15901 ttgcatattt cccccgttca gttttgcagg tgagttttga acaaatgtat ttctgctttt
    15961 aatttgttca taaccttagg tggacattgc acatatttcc gacgaataga tcacaattta
    16021 tgctattttg attttcacgg ttgcgtttgt tcatgctcgt agagtcaaac tgtggctgcc
    16081 atcacagata taaatgcgca agaactgaac aattgcatta aagatttaaa tatgttcaaa
    16141 gtgaagaatg aattatgaca atcaacgatt cggcaatttc agaacaggga atgtgtgaag
    16201 aagaacaggt cgcgcggatc gcgtggtttt actatcacga cgggctgacc cagagcgaga
    16261 tcagcgatcg tctcggcctg acccgtttga aagtgtcgcg cttgctggag aaagggcatc
    16321 agtccggcat tattcgcgta cagattaatt ctcgctttga aggctgtctg gaatatgaaa
    16381 ctcaattacg tcgtcagttt tcgctgcaac atgtccgggt gatccctggg cttgcggatg
    16441 ctgatgtcgg cgggcgactg gggataggcg cggcgcatat gttgatgagt ttacttcaac
    16501 cacaacagat gctggcgatt ggttttggcg aggcaaccat gaatacgctg caacgcttaa
    16561 gtggttttat ttcgtcacag caaattcgcc tggtcacgct ctccggtggc gtcggttctt
    16621 atatgacggg tatcgggcag cttaacgcgg cgtgcagtgt gaatattatt ccggctccgt
    16681 tgcgggcatc ctccgctgac attgcccgta cgctaaaaaa tgaaaattgc gtcaaagatg
    16741 ttctgttagc cgcgcaagca gccgatgtgg cgattgtcgg cattggggcc gtcagtcaac
    16801 aggacgacgc gacgatcatt cgctccggtt atatcagcca gggcgaacag ttaatgattg
    16861 gtcgaaaagg ggcggttggc gacattttag gctacttttt tgatgcaaaa ggtgacgttg
    16921 tcacggatat taaaatacat aacgaactga ttggcttacc tttaagctcg ctgaagacca
    16981 tacccgtccg ggttggcgtg gcagggggag aaaataaagc cgaagcaatt gccgctgcaa
    17041 tgaaaggcgg ttatatcaac gcactggtta ccgatcagga cacagcagcg gcgattttac
    17101 gtagttaaat ttgcatgacc ttattaccgg gaatgatgga gaacgtatag aggaacagac
    17161 agaacgcata agccgaggat aatctaatgg ctcgactctt taccccttca gaatcaaagt
    17221 attacctgat ggcgctggat gcaggtaccg gaagtattcg ggctgtgata ttcgacctgg
    17281 aaggcaatca aatagcagtg ggacaggcgg agtggcggca cctggcagta ccggacgttc
    17341 ctggttctat ggaatttgat ctcaacaaaa actggcaact ggcgtgtgag tgtatgcgcc
    17401 aggcgctgca caacgccggc atagccccgg agtatatcgc tgccgtttcg gcatgttcga
    17461 tgcgtgaagg cattgtttta tataataatg aaggagcccc gatctgggcc tgcgccaatg
    17521 tggatgccag agcggcacgc gaagttagcg aacttaaaga actgcacaac aatacctttg
    17581 aaaacgaagt ttatcgcgcg accggacaaa cactggcttt aagtgccatc cccagattac
    17641 tttggctggc gcaccatcgt tccgatattt accgtcaggc atcaaccatc accatgatca
    17701 gcgactggct ggcctatatg ctcagcggcg aactggcggt ggatccctct aacgctggca
    17761 ccacgggact tcttgatcta accacccgtg actggaaacc tgcattgctg gatatggctg
    17821 gcctacgtgc cgatattctt tctcctgtca aagaaaccgg cacattgctg ggcgtggtaa
    17881 gttcacaagc ggcggaactc tgcggtctga aggcgggcac tccggtggtc gttggaggag
    17941 gcgacgtgca gcttggttgc cttgggttag gcgttgtgcg tccggcacaa accgcggttc
    18001 ttggcggcac attctggcag caagttgtaa atttagccgc gccggtgaca gacccagaaa
    18061 tgaacgtgcg cgttaatcct catgttattc ctggcatggt acaagctgaa tctataagct
    18121 tttttaccgg actcaccatg cgctggttcc gcgatgcttt ctgtgccgaa gaaaaactga
    18181 ttgccgaacg tttaggcatc gacacctata cgctgctgga agagatggcc agtcgggtgc
    18241 cgcctgggtc gtggggcgta atgccgatct tctccgacag aatgcgcttt aaaacctggt
    18301 atcacgctgc gccttccttt attaacttgt ccattgaccc ggataaatgt aacaaagcga
    18361 cattgttccg tgcgctggaa gaaaatgcgg cgattgtatc agcgtgtaac ttgcagcaaa
    18421 ttgctgattt ctcgaatatt catccttcat cgttagtctt tgcaggcgga ggttcaaaag
    18481 ggaaattatg gagtcaaatt ctcgctgatg tctcgggatt acccgtcaat attccggtgg
    18541 tcaaagaagc cactgcatta ggatgtgcca ttgcagctgg cgtcggtgcc ggaatttttt
    18601 catcaatggc agaaaccgga gaacgcctgg ttcgctggga acggacgcac acaccagacc
    18661 cggaaaagca tgaactttat caggattcac gcgataagtg gcaggcagtt tatcaggatc
    18721 agctggggct ggttgatcat ggactgacga cgtcgttatg gaaagcgcct gggttatagt
    18781 ttcattgaca gccacatcat gatgaccgtg gctgttagtt tttgagtata gttattctta
    18841 tttcccttct tttagagctg gcttgttaaa agccagcttg cagattcatc gttccatcac
    18901 cagtactata ttcaagggaa aattgctatt tttcttaaag cattaagcac aacccttatt
    18961 ttatagcgaa tattcctaaa gacactaatg gatgcaaaag gggtttaata aacttcgatt
    19021 tttagcaatt agtgctattc tttgttaaat atgatgatga taattttcct attgaaagaa
    19081 acagttttct tattaagcaa caataactca tagttcatgt ttaaataaaa taagaactat
    19141 gcaattttta atctatcaat tcgaaagcat tagcaaatta gccaaattga caccttccca
    19201 cctccaacta acactcaata cagacctctg acaatttaat aagtttcatt tttaatgtct
    19261 gacgactcat ctctttcgtg gctgtattgt ttgtatatgg agcgtttata tgaatagaat
    19321 ctatcgcgtg atatggaatt gcactctaca ggtatttcag gcctgctcgg aattaactcg
    19381 cagggcaggt aaaacatcga cggttaattt gcgtaaatcc tctggactga caacgaaatt
    19441 cagtagattg acgctgggtg ttttgctggc actaagcggt tcagcgtctg gtgcaagtct
    19501 ggaagttgat aatgatcaga ttaccaatat tgatactgat gttgcttatg atgcctacct
    19561 ggttggctgg tatggcactg gagtgcttaa tattttggct ggcggtaatg cctccttaac
    19621 cactattact accagcgtca ttggcgctaa tgaggactca gagggcaccg ttaatgtttt
    19681 gggtggcacc tggcgattgt atgatagcgg aaataatgca aggcctttaa atgtgggtca
    19741 atccggaacg gggacgctga atattaaaca gaagggtcac gtcgatggag gctatttaag
    19801 attaggttct tcgacaggag gcgtcgggac ggtcaatgtt gagggagagg actctgttct
    19861 gacgaccgaa ttattcgaaa tagggagtta cggtacgggc tcattaaata ttacggataa
    19921 gggttacgtc acgagttcaa tcgtcgccat tttaggctat caggcgggca gtaatggcca
    19981 ggttgtcgtt gaaaagggtg gcgagtggct aataaaaaat aacgattcct caattgaatt
    20041 tcaaattggt aatcaaggaa cgggggaagc gactattcgc gagggtggac tgattacggc
    20101 tgaaaacacg attatcggtg gcaatgctac cggtatcgga accctgaatg tgcaggatca
    20161 agactctgtc atcacggtac gcagactcta taatggatat ttcggtaatg gcacactcaa
    20221 tatttccaat aatggactaa ttaataacaa agaatattca ttagtgggcg ttcaggacgg
    20281 ttcccacggt gtcgtcaacg tgaccgataa agggcattgg aatttcctcg gaacgggcga
    20341 agctttccgc tatatctata tcggtgatgc tggcgacggt gaacttaatg tctcgcgtga
    20401 aggtaaagta gattcgggaa ttatcactgc ggggatgaaa gaaacaggca caggcaacat
    20461 tactgttaag gataagaact ccgttatcac taatctcgga actaatcttg gttatgacgg
    20521 ccacggcgaa atggatatca gtaatgaggg gcttgttgtc agcaacggag gaagctcact
    20581 cggttatgga gaaaccggcg tcgggaatgt cagcatcacc acggggggga tgtgggaggt
    20641 caataagaat gtctatacca ccattggtgt tgcgggcgtc ggaaacctca atattagcga
    20701 tggcggtaag ttcgtatcgc aaaatattac ttttttgggc gataaagcaa gcggtatcgg
    20761 cacactgaac ctgatggatg cgacatcatc gttcgatact gtgggtatca atgtcggtaa
    20821 ttttggtagc ggtatcgtaa atgtcagtaa tggtgccacc cttaattcaa cgggctatgg
    20881 atttatcgga ggaaatgcct ccggtaaggg gatagttaat atttcaacgg acagtctctg
    20941 gaatttaaag acatcatcta ctaacgcaca attgctacag gtcggtgtat taggcaaggg
    21001 tgaactgaat attaccaccg gaggtatagt taaagcgcgt gatacacaga tagctctcaa
    21061 tgacaaaagt aagggcgacg tgagggtgga tgggcagaac tctcttcttg aaacattcaa
    21121 tatgtacgta gggacatctg gtacgggtac gttaaccctg acgaatagcg gcacgctgaa
    21181 tgtcgaaggt ggagaagttt acttaggtgt ttttgaacct gctgtaggaa cgctaaacat
    21241 tggtgcagct cacggtgagg cggcggcaga tgccgggtat ataaccaacg cgacgaaagt
    21301 agagtttggt tctggcgaag gtgtttttgt ctttaatcat actaataaca gtgatgccgg
    21361 ctaccaggtc gatatgctga ttacaggtga cgataaagac ggaaaagtga tccatgatgc
    21421 aggccatacg gtgttcaatg cagggaatac ttatagcggt aaaacgctgg tcaatgacgg
    21481 cctcctgacc attgcgtctc atacggcaga tggggtaacg ggcatgggtt cgagtgaagt
    21541 caccattgca agccccggta cgctcgacat tctcgcatca acgaacagtg caggagatta
    21601 cacgctgacc aatgcgctca aaggcgatgg cttgatgcga gtgcagctgt catcctacga
    21661 caagatgttt ggctttaccc atgcaacagg gactgaattc gccggtgttg cccaactgaa
    21721 agacagtacc ttcactctgg aacgcgacaa caccgctgcg cttactcacg cgatgttgca
    21781 gtctgacagt gaaaatacca catcggtaaa agtgggagag caatccattg gtggactggc
    21841 tatgaatggc ggtacgctca ttttcgatac ggatattcct gctgcgacgc ttgcagaggg
    21901 atatatcagc gtcgatacgc tggttgtcgg cgcgggtgac tacacctgga aaggccgtaa
    21961 ctatcaggta aacgggacgg gcgacgtgct tatcgacgtg cctaaaccgt ggaatgatcc
    22021 catggcgaat aaccctctga cgacgctcaa tttgctggaa cacgacgata gccatgtcgg
    22081 cgttcaactg gtgaaggcgc aaacggttat tgggtcgggt ggctcattaa cgttacgtga
    22141 tttacagggc gacgaggtgg aagcggacaa aacgttacac attgcgcaaa acggaacggt
    22201 ggtcgccgag ggtgattatg gattccgcct cacgaccgca ccaggtgatg gtttgtacgt
    22261 taactatggg ctgaaagcgc tgaacatcca tggtgggcaa aagctgacgt tagccgaaca
    22321 tggcggagcc tatggcgcaa cggccgatat gtcggcaaaa atcggtggtg aaggggatct
    22381 ggcaatcaat acggtgcgac aggtttcgct ttccaacggt cagaacgact atcagggggc
    22441 aacctacgtt cagatgggga cattacgtac cgatgcggat ggtgcacttg gcaacacccg
    22501 ggaactgaac atcagcaacg cggccatcgt cgatcttaat ggatcgacgc agacggtaga
    22561 gacattcacc gggcagatgg gttcgactgt tttgttcaaa gagggggcgc tgacggtaaa
    22621 taaaggtggg atcagtcagg gtgaactgac aggtggcgga aacctgaatg ttacaggggg
    22681 aacgctggct atcgaggggc ttaatgcacg ctacaatgcg ttaaccagca ttagcccaaa
    22741 tgcggaagtc agcctcgata atactcaggg gttaggcaga ggaaatattg ccaatgacgg
    22801 tctgttaacg ctaaaaaacg tgactggcga actgcgtaat agcataagcg ggaagggtat
    22861 cgtgagcgca accgccagga cagatgtaga gctggatggc gataatagcc gctttgtggg
    22921 gcaattcaac attgatacag gcagcgcgct cagcgtcaac gagcagaaaa acctgggtga
    22981 tgcttccgtt atcaataatg gcctgctcac catctccact gagcgtagct gggcgatgac
    23041 gcacagtatc agcggtagcg gtgatatgac aaaactgggt accgggatcc tgactcttaa
    23101 caacgattcc gcggcgtatc agggtacgac ggatatcgtg gggggggaaa ttgctttcgg
    23161 ttccgactct gccattaata tggcaagtca acacattaat atccataaca gcggtgtgat
    23221 gtcgggaaat gtcaccactg caggtgatgt gaacgttatg cctgggggga cactgcgtgt
    23281 cgctaaaacc acaatcggcg gcaacctgga gaatggcggc acggttcaaa tgaatagcga
    23341 aggggggaaa ccggggaatg tactgaccgt taacggcaac tataccggaa acaatggcct
    23401 gatgacgttc aacgcgacgc tgggcggcga taattcgccc accgataaaa tgaacgtgaa
    23461 aggcgatacc caagggaaca ctcgcgttcg ggttgataac attggcggcg tcggtgcaca
    23521 aacggtcaac ggtattgaac tcattgaggt tggcggtaat tctgcaggta acttcgcgct
    23581 gaccaccgga actgtcgaag ctggggctta cgtctacacg ctggctaaag ggaaggggaa
    23641 tgacgagaaa aactggtatc tgaccagtaa atgggacggc gtaacgccag cggatacgcc
    23701 cgaccccatc aataatcccc ctgttgtgga tccggaaggc ccatcagttt atcgcccgga
    23761 ggccggaagc tatatcagca acattgccgc agccaactcg ctgtttagcc atcgtttaca
    23821 cgaccgtctg ggtgagccgc agtatataga ttcactgcat tctcaggggt cggcaagcag
    23881 tatgtggatg cgtcatgtcg gagggcacga acgttcaagg gccggtgacg gtcagctaaa
    23941 tactcaggct aaccgctatg tattgcagct aggcggcgat ttggcgcagt ggagtagcaa
    24001 cgcgcaggat cgctggcatc ttggcgtgat ggcaggttac gctaatcagc acagtaatac
    24061 tcagagtaat cgtgtgggtt ataaatcgga tgggcgcatc agcggttaca gcgctgggct
    24121 gtacgcgacc tggtatcaga acgatgcgaa taagaccggc gcttatgttg acagctgggc
    24181 gctgtataac tggtttgata acagcgtcag ttccgataac cgttctgctg acgactatga
    24241 ttctcgcggt gtgacggcct ctgttgaggg tgggtatacc tttgaagcgg gaacatttag
    24301 cggcagcgaa gggacgctga atacctggta cgtccagcca caggcgcaaa tcacctggat
    24361 gggtgtgaaa gattccgacc atacccggaa agacggaacg cgcattgaaa cggaaggcga
    24421 cggaaatgtg caaacgcgac ttggggtgaa aacctacctg aacagccatc accagcgtga
    24481 cgatggtaaa cagcgtgagt tccagcctta cattgaagcg aattggatca acaacagcaa
    24541 agtctacgcc gtgaagatga atggtcaaac cgtaggccat gaaggtgcgc gtaatctcgg
    24601 tgaagtacgt accggggttg aggcgaaagt aaataacaac cttagcctgt gggggaatgt
    24661 cggtgtgcaa ctaggtgata aaggctatag cgatactcag ggcatgctgg gagtgaaata
    24721 tagctggtaa accgtataag ccgcatgtcg agatggcatg cggcttaata ttgccgactt
    24781 caaaggcttt aaagcctccc ttttatccgc gatcgcggat atcgcagcgt ttatcccgta
    24841 cagcggataa gatgtgtttc cagattgact ttatcctcac taaaggataa aactgataat
    24901 atccccttaa gcggataaat ttgctgtgga cgtatgacat gatgagcttt cagaagatct
    24961 atagcccaac gcaattagcg aatgcaatga aactggttcg ccagcaaaat ggctggacgc
    25021 agagcgagct ggcgaaaaaa attggcatta agcaggcgac aatttccaat ttcgaaaaca
    25081 accctgacaa taccacgctc acgacatttt ttaagatttt acagtcgctt gaactctcaa
    25141 tgacactatg cgacacgaaa aatgcctcgc cagaatcaac agaacagcaa gatctggagt
    25201 ggtaatgcct aaacttgtca cttggatgaa caaccagcgg gtaggcgagt taacgaagtt
    25261 agccaacggc gcgcacacct ttaagtatgc accggagtgg ttagccagcc gttatgccag
    25321 accgttgtca ctttcgctgc cattgcagag ggggaatatc acctcagatg ccgtatttaa
    25381 cttcttcgat aacctgttac ccgatagccc gattgtacgt gaccggatcg ttaaacgtta
    25441 tcatgctaaa tccagacaac cgtttgattt attgtcagaa atagggcgag acagcgttgg
    25501 tgccgtaacg ttattacccg aaaatgaaac cataacgcgc ccgataatgg catgggaaaa
    25561 gcttactgaa gccaggcttg aagaagtatt aacggcttat aaagcagata tccctctggg
    25621 catgattaga gaagaaaatg actttcgcat ctcggttgct ggcgcgcagg agaagacagc
    25681 gctgcttaga ataggcaatg actggtgcat tccgaaagga ataacgccga cgacgcacat
    25741 cattaaatta ccgattggcg aaatcaggca gcccaatgcg acgctcgatc tcagccaaag
    25801 cgttgataat gagtattact gtctgctgct ggcgaaagaa cttgggttga atgttccgga
    25861 cgcagaaatc attaaagcgg gaaatgtgcg cgcgttagcg gtcgaacgtt ttgacaggcg
    25921 ttggaatgct gagcgaacgg ttttacttcg cttgccacag gaggatatgt gtcagacatt
    25981 cggtttacct tcatcggtga aatatgaatc agatggaggc ccaggcatcg cgcggatcat
    26041 ggcttttttg atggggtcca gcgaggcgct gaaagatcgc tatgatttta tgaaattcca
    26101 ggtcttccag tggttgattg gcgcaacgga cggtcatgca aaaaacttct ccgtatttat
    26161 tcaggctggc ggcagttatc gactcacgcc attttacgac atcatttcag catttccggt
    26221 ccttggcggt acgggaatac acatcagcga tctcaaactg gcaatggggc ttaacgcatc
    26281 caaaggcaaa aaaacggcaa tcgataaaat ttatccgcga cattttttgg cgacagcaaa
    26341 ggtgctgaga ttcccggaag tgcagatgca tgaaatccta agtgactttg ccagaatgat
    26401 tccggcagca ctggataacg tgaagacttc attaccgaca gattttccgg agaacgtggt
    26461 gacggcagtt gaaaccaatg tgttgaggtt gcatggacgg ttaagccgag aatacggtag
    26521 taagtgagat atggggtttg gtcgttgtta atgagcacga caatcatgac cgccaacgac
    26581 tggattcttc cttggtttat actggtgcgc ataatgtata ttatgttaaa tcatgtctga
    26641 gtgatgcgaa gtgcttttct ggtagtcgtt attcgttcag ggttcaagaa cttaagcctc
    26701 tcctctttca taattggcct gtgcatggag aaatagcctg gttattccct gccccatgct
    26761 aagagcgcag tgtctgtcgc atagacactg catacgaaaa caccagaggg tgctgttact
    26821 tatttaagga cggatcaagc gggaatgagt ccactccggt atcagaaatg gctacgtctc
    26881 aatgaagtca ggcgaccgat gctgaatgaa cattacgatg tcaccacagc agcctatgct
    26941 gtcggttacg aaagctatcc catttcagtc gggaatattc gcggatgttt ggagagtcac
    27001 ccaagagaga tattaccagg ttaagaaagt ctgtcggtca gctttaataa accctgcact
    27061 tatctgttta gagcaattcg gtgttagttt cagcaagcaa acattaacca tagctaatga
    27121 tttatagcca tattaaccat tagtagccct tgacctttcc tgacacttcc cttgttcgca
    27181 ataatattct gtttagtcac ggtttataaa taacatgcat aatgaaatcg tcatgttatt
    27241 tagcgacata aaatgttttt gcaaatactc tcttcaacaa gtgcagcaat aaacttgttg
    27301 tgattaggtg tccttgtgat ttaaagatat tatcgttctt cgtgaaatgt aataaccctg
    27361 gaaactatat tctcagggca acaaccaatc aagaatgatt cttgttttat aagcaataac
    27421 agaatgactt aattgttaac tctctttatt cagctactta aatataaatt ttggagaatt
    27481 atgaaactca aacatgttgg tatgattgtc gtttctgtgt tggcgatgtc gtctgctgcg
    27541 gtaagcgcag ccgagggtga tgaatcagta acgaccactg ttaatggcgg tgttattcat
    27601 tttaaaggtg aagtggtaaa tgccgcttgt gcaattgatt cagaatcaat gaaccaaacg
    27661 gttgagctgg gtcaggttcg ttcttctcgc ctggctaaag cgggtgacct cagctccgcc
    27721 gttggcttca atatcaagct gaatgattgt gataccaatg tttccagtaa tgcagctgtt
    27781 gcattcctgg gtactactgt caccagtaat gacgatacgt tagcgctgca gagttcaacg
    27841 gcaggctctg cccaaaatgt cggtattcaa attttggacc gtacgggtga ggtattaata
    27901 cttgatgggg ccacttttag tgctaaaacc gacttgattg atggcacgaa tatactgcca
    27961 ttccaggctc gttatattgc tctcgggcag tccgtagctg gtactgcaaa cgctgatgcg
    28021 accttcaaag ttcaatatct ataattcaat cttaaggtgg ctatacgtca tctgaaattg
    28081 aaacagggtg tgtgtggcat ggctacgcac acccgagcaa tttaccaata ttcatctcat
    28141 ttttaatgag aaatggtttg tatttggagc gctatgaaaa atcacataat atctaaacta
    28201 tcaataaaca acagtagtta aacttactgt tatttaaaat atttactttg tagcaaggtt
    28261 ttcctattaa aagagaacca acatttatta caaacgcatt aagtctggaa acccggcgac
    28321 tcgccaatgt attagcaaca ttaatcgatt gtctgatgta acggttttcc cgcttatcct
    28381 cttaacacat ggagaatgca tcagatgcaa actacgcgta ccccctactc tatttccttt
    28441 atggcgacag tgttactttt attgctcttt gcttgccata gcaccgttgc caacgcagct
    28501 gtcgcactgg gtgcgacgcg ggtaatttat ccggcaaacc agaaacaggt tttattgcct
    28561 gtcacgaata atgatcctgc cagtgtctat ctcattcagt catggataga aaatgcaggt
    28621 gaccaaaaag acacccagtt tgtcattacc ccaccgcttt tttccatgca aggtaaaaaa
    28681 gagaatacgc tgcggataat caacgctact aatcatcagt taccaggcga ccgtgaaagc
    28741 ctgttctggg tgaatgttaa agctattcct gctatggaaa aagaccagaa gaatgagaat
    28801 acattacagc tggcaattat tagccgcata aaaatgtttt atcgccccac gaacctggca
    28861 atggcacctg aagaagcgcc tgcaatgctt cggtttcgtc gcagcggtag caaactcacg
    28921 cttattaatc caacgcccta tttcattacc gtaacaaata tgaaagcagg aaatagcaat
    28981 ctcccaaaca ccatggtgcc gccaaaaggt gaagtatcgg tggatatcac gcatgctgca
    29041 accggggata tcagttttca gacgatcaat gattacggtg cattaactcc gcgtattaag
    29101 gcaaccatgc agtaagccag tgacctaatt aataataaac tcaatcggaa ttaataatga
    29161 cagccattcg tgccgcattt aaggcatatc gtatgcatca ggtgttaata atgcctcgct
    29221 ttgcacggct taccattgcc cttggcttag caactgcggt atttcccgtt gacgcagagt
    29281 attattttaa tccacggttt ttaagcaatg atcttgctga atctgtcgat ttatccgcct
    29341 ttactaaggg acgcgaggca ccacctggta catatcgggt cgacatttat ttgaacgatg
    29401 aatttatgac tagtcgggat attactttta ttgcagatga taataatgca gaactcatcc
    29461 catgcctgag tacagatctt cttgtcagtc ttggtataaa aaaatcagcg ttattagaca
    29521 ataaagaaca ttcggcagaa aaacatgtgc cggacaacag cgcatgcaca ccacttcagg
    29581 atcgtttggc cgacgcatca actgagtttg atgttggtca gcaacatttg tcactctccg
    29641 ttccgcaaat ttatgttggc agaatggctc gcggctatgt ttctcctgat ctgtgggaag
    29701 aaggaataaa tgctgggctt ctaaactata gttttaatgg taattctatt aataatcgta
    29761 gtaaccataa tgcaggaaaa tcaaactatg catatttgaa tttacagagt ggcatcaaca
    29821 ttggtagttg gcgactgcgc gataactcaa cgtggagtta ttacagtggg agtagcaatt
    29881 catctgacag caataaatgg cagcatatca atacgtcggc tgaacgtgac attattccct
    29941 tacgctcacg gttaacggta ggtgatagtt ataccgatgg cgatattttt gatagtgtga
    30001 actttcgtgg cctcaaaata aattcaacag aagcgatgtt gcccgatagc caacatggtt
    30061 tcgctccggt gattcatggt attgcccgtg gaaccgcaca agtgagtgta aaacagaacg
    30121 gatacgatgt ttatcagact actgttccac ccggcccttt tactattgat gatatcaact
    30181 ctgcggccaa tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc agtattcaga
    30241 cattatatgt tccttattcg tctgttccgg ttctccaacg tgctggatat acgcgttatg
    30301 cgcttgccat gggggaatat cgtagtggaa ataacctgca aagctctccc aagttcatac
    30361 aaggtagctt gatgcatggt ctggaaggaa actggacacc ttatggcgga atgcaaattg
    30421 cagaagatta tcaggccttc aaccttggta ttgggaaaga tttaggactt tttggtgcct
    30481 tttctttcga tatcacgcag gccaatacga cacttgcaga tggcacccgt cacagtgggc
    30541 aatcggttaa atccgtctac agcaaatcct tctaccagac aggaaccaat atccaggtcg
    30601 caggatatcg ctattctacg caaggttttt ataacttatc cgacagtgcc tacagtcgaa
    30661 tgagtggtta caccgtcaag cctcctaccg gagacaccaa tgagcagaca caatttattg
    30721 attattttaa tctgttctac agtaagcgtg gtcaggaaca aataagcatc tctcagcagc
    30781 ttggaaatta cggtacgaca tttttcagtg ccagtcgcca aagttactgg aacacgtcac
    30841 gcagcgacca gcaaatatca tttggattaa atgtgccgtt tggtgatatt acgacttcgc
    30901 tgaattacag ctattccaat aatatatggc aaaacgatcg ggatcattta ctcgctttta
    30961 cgcttaatgt tcccttcagt cattggatgc gtacagacag tcagtcggca tttcgtaatt
    31021 caaacgccag ttacagtatg tcaaacgatt tgaaaggcgg catgaccaat ctatcggggg
    31081 tttatggcac tctgctgccg gataataacc tgaattatag cgttcaggtc ggtaacaccc
    31141 acggaggtaa tacatcgtct ggcaccagtg gttacagttc tcttaattat cgtggagctt
    31201 acggtaatac gaatgtcggt tacagtcgga atggtgacag cagccagatt tattacggaa
    31261 tgagtggtgg gattattgct catgctgatg gcatcacctt tggacagccg ctgggcgaca
    31321 caatggttct ggttaaggct cctggcgctg ataatgtcaa aatagagaac cagaccggaa
    31381 ttcataccga ctggcgtggc tatgcaatat taccatttgc gacagaatat agagaaaatc
    31441 gtgtcgctct taacgcgaat tcccttgcag ataatgttga actggatgaa accgtagtca
    31501 ctgtcatccc aactcacggt gctattgcca gagcaacatt taatgcacaa atcggcggga
    31561 aagtattaat gacgttgaag tacggtaata aaagcgttcc attcggtgca attgtcactc
    31621 acggagagaa taaaaatggc agcattgtcg cggaaaatgg tcaggtttat ctgactggac
    31681 ttcc
//
LOCUS       DAFATA010000046        31161 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFATA010000046 DAFATA010000000
VERSION     DAFATA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.047"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1286)
                     /locus_tag="LGJ20_002554"
     CDS             complement(<1..1286)
                     /locus_tag="LGJ20_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2386600.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLTFHRAAEGDVAGAVTGVTDGAGRCFHLVLTTQAQRAEAFRKQRATSLSSPAGPRSA
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     ERRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGS"
     gene            complement(1362..3503)
                     /locus_tag="LGJ20_002555"
     CDS             complement(1362..3503)
                     /locus_tag="LGJ20_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBK2386601.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDSP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(3713..4231)
                     /locus_tag="LGJ20_002556"
     CDS             complement(3713..4231)
                     /locus_tag="LGJ20_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBK2386602.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            4927..5427
                     /gene="tssB"
                     /locus_tag="LGJ20_002557"
     CDS             4927..5427
                     /gene="tssB"
                     /locus_tag="LGJ20_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBK2386603.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            5462..5686
                     /locus_tag="LGJ20_002558"
     CDS             5462..5686
                     /locus_tag="LGJ20_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386604.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            5737..7212
                     /gene="tssC"
                     /locus_tag="LGJ20_002559"
     CDS             5737..7212
                     /gene="tssC"
                     /locus_tag="LGJ20_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HBK2386605.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            7219..7632
                     /gene="tssE"
                     /locus_tag="LGJ20_002560"
     CDS             7219..7632
                     /gene="tssE"
                     /locus_tag="LGJ20_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBK2386606.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            7636..9486
                     /gene="tssF"
                     /locus_tag="LGJ20_002561"
     CDS             7636..9486
                     /gene="tssF"
                     /locus_tag="LGJ20_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBK2386607.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            9450..10532
                     /gene="tssG"
                     /locus_tag="LGJ20_002562"
     CDS             9450..10532
                     /gene="tssG"
                     /locus_tag="LGJ20_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBK2386608.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            10557..11837
                     /gene="tagH"
                     /locus_tag="LGJ20_002563"
     CDS             10557..11837
                     /gene="tagH"
                     /locus_tag="LGJ20_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBK2386609.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQHSIPAQAFTIILHDGTFCLRPQTVQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MMRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINSPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPKNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            11834..12358
                     /gene="tssJ"
                     /locus_tag="LGJ20_002564"
     CDS             11834..12358
                     /gene="tssJ"
                     /locus_tag="LGJ20_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBK2386610.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            12361..13692
                     /gene="tssK"
                     /locus_tag="LGJ20_002565"
     CDS             12361..13692
                     /gene="tssK"
                     /locus_tag="LGJ20_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBK2386611.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TELSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            13697..14458
                     /gene="icmH"
                     /locus_tag="LGJ20_002566"
     CDS             13697..14458
                     /gene="icmH"
                     /locus_tag="LGJ20_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HBK2386612.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQEQGEFEQIYRRLYHVLHKLRGNAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILYQLNKLLR"
     gene            14467..17226
                     /gene="tssH"
                     /locus_tag="LGJ20_002567"
     CDS             14467..17226
                     /gene="tssH"
                     /locus_tag="LGJ20_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBK2386613.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAAQLLTGINRDRLQQDFVQWTQESAESV
                     VPDADCKGAGALTDVADSLLARYAKNMTEDARNDRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTNTSRMTEVLVQYDETLTALDELEAAWHQQQTLVQEIIALRQQLLGMAEDD
                     AASLPHVDAVEDTPPESEQDNTGAEPADEAGSEQPEETAETVSPVQRLAHLTAELDAL
                     HNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIA
                     SLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSE
                     FQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAF
                     DKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALL
                     ARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAEN
                     GARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESVTK
                     DETVL"
     gene            17223..17966
                     /gene="tagO"
                     /locus_tag="LGJ20_002568"
     CDS             17223..17966
                     /gene="tagO"
                     /locus_tag="LGJ20_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBK2386614.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            17971..19383
                     /gene="tssA"
                     /locus_tag="LGJ20_002569"
     CDS             17971..19383
                     /gene="tssA"
                     /locus_tag="LGJ20_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBK2386615.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            19492..22926
                     /gene="tssM"
                     /locus_tag="LGJ20_002570"
     CDS             19492..22926
                     /gene="tssM"
                     /locus_tag="LGJ20_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBK2386616.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKALNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            22937..24289
                     /locus_tag="LGJ20_002571"
     CDS             22937..24289
                     /locus_tag="LGJ20_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBK2386617.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            24313..24795
                     /locus_tag="LGJ20_002572"
     CDS             24313..24795
                     /locus_tag="LGJ20_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBK2386618.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            24839..25754
                     /locus_tag="LGJ20_002573"
                     /pseudo
     CDS             24839..25754
                     /locus_tag="LGJ20_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25764..26243
                     /locus_tag="LGJ20_002574"
     CDS             25764..26243
                     /locus_tag="LGJ20_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386619.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNVHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(26380..27165)
                     /locus_tag="LGJ20_002575"
     CDS             complement(26380..27165)
                     /locus_tag="LGJ20_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK2386620.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(27497..27573)
                     /locus_tag="LGJ20_002576"
     tRNA            complement(27497..27573)
                     /locus_tag="LGJ20_002576"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27537..27539),aa:Asp,seq:gtc)
     gene            complement(27706..28437)
                     /gene="dnaQ"
                     /locus_tag="LGJ20_002577"
     CDS             complement(27706..28437)
                     /gene="dnaQ"
                     /locus_tag="LGJ20_002577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBK2386621.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            28502..28969
                     /gene="rnhA"
                     /locus_tag="LGJ20_002578"
     CDS             28502..28969
                     /gene="rnhA"
                     /locus_tag="LGJ20_002578"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBK2386622.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(28966..29688)
                     /locus_tag="LGJ20_002579"
     CDS             complement(28966..29688)
                     /locus_tag="LGJ20_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK2386623.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            29722..30477
                     /gene="gloB"
                     /locus_tag="LGJ20_002580"
     CDS             29722..30477
                     /gene="gloB"
                     /locus_tag="LGJ20_002580"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBK2386624.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDLDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            30549..>31161
                     /locus_tag="LGJ20_002581"
     CDS             30549..>31161
                     /locus_tag="LGJ20_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBK2386625.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMK"
BASE COUNT         7530 a         8315 c         8132 g         7184 t
ORIGIN      
        1 atgctcccgt ccgcatgctc cttcttcacc acccgcttca ggccgccttc gccctccgtg
       61 tacagcacct cccgccggtt caggctgtcc gtgatgatta cccggcgctc tccgtactca
      121 aagcggtagt ccagcccctc cgggttgacc tgctccgtca cccgaccggt atcatcatac
      181 cggtagcggc tctccggcct ccccgcataa tggtgtgcca ccatccgccc ggcgtgctcc
      241 gcatcataag taaacccgcg aacctgcgtc ccgctgcggt catacaccgc ccgcagttct
      301 ccgctggccg tgtacgtgta gcgcgccagc ggcgcagtcg gctgttcatc cgggtatgcc
      361 gggtcgtgtg tcagccacac cgcctccagc cggataccgt tatcggcacc gtattctgta
      421 ccggcgggca gcgtgtcggg gaaagcagaa gaagaggaag cagaacgggg accggcaggg
      481 gaagataaag acgtggcgcg ctgtttacgg aaggcttccg cccgctgcgc ctgtgtggtc
      541 agcaccaggt gaaaacagcg gcccgcgccg tccgtcaccc ccgtcaccgc gcccgccacg
      601 tcaccctccg ccgcccggtg gaaagtcagc gtccgcccga agccgtccac cacgcccgtc
      661 agcacccggt atgcgggcgg ctccggcggc agcacctcgt ccgcccccgg cacccgctcc
      721 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      781 ctcaggcgaa catcttccgg cagcacctgc cagagtgcgc ttagcggctg cgaactgtgc
      841 tgctccgcca ccccgccccg cgccagccag agtgactcgc tgcggctgta gcttatctcg
      901 ccgggaaaca gcggctcaaa gtgaatgctc ctgccgccgt tgtcgttgag tatcaggcct
      961 tcatcgcgta tctgtaagcg gatatcgaag ggcgctttcc agccgggacc aaacaccccc
     1021 accggcgcgg gtgttctggt ccggtagctg ctgtaggcgc gggaaagaat aaacggcagc
     1081 gggccgggca gcgcaaggtc cgtctcgccc ggcagcacct tcgcacccag cagcgggttc
     1141 accgggttag cataggtaat ccctcccgga cataccgaac aggccactcc cgtcggcgca
     1201 cctatcagca cccccgctga accctgcaca atatccagtc ccttgcgggt catatcaccc
     1261 tgccgcgccg ccggttttcc gcccatgaat caattccttc tgcttttaat atggtttatc
     1321 tggtcactgc accagttccg ttttaatttt ttgcacctga ctcaaaattg ccgcatatcc
     1381 ggcattccgg ggggtaaatc tttcggcaca ggcgcatccg gtacaggttg tgtggcacca
     1441 cagttccagt taatcactgg cgaatcaccg ttcacatccg actccccttc cagatgaatg
     1501 tgcgtaccat tgagaaatat gcttccgtcc ttcgtcagca ccagccttgc cttaccacag
     1561 cagagttcaa gatgctcacc ggcagaataa actgctgttt gtccggtgtt ttccttcatt
     1621 gtcttgccca ccgagaaggt gacgttattc cccacattga cgctgtagcc cattcccacc
     1681 atcagtgatt tatgcagccc tacctgtgag gactgcaaca acgccaccga ggtattcata
     1741 atgccgccta ccgtcgtctg gtaagccaca cccaccgtca gcgcacgaat gatccccacc
     1801 ttttcaacct ggtttgatcc cactttgata atctggttac tgcccaccgt ttccacctgg
     1861 ttgacaccaa ccgtctgtat ctgattaacg ccaatgttct ggatctggtt attggtaacg
     1921 gtgatcgcct ggttgttacc tattttttcg gcatggttgt ttatcacatc agtggtgcgg
     1981 ttattcagca cctcggtgtt catgttcttc tgcgcgtgga tgtagacctg ttctttccct
     2041 gtcgcatcgt caaacttcag ttcattaaac ccgctgccct tataggtttt cgagcggatc
     2101 gtcatctgcg tcttcgtccc cggcaggctg ccgggggtgc ggttttcctg gtggtaggta
     2161 cgccccataa tgatcggctg gtccggatcg ccgttgagga agtccacaat gacctcctga
     2221 cccacacgcg gtatcgccag gttaccaaag ccggtgcctg cccacgcctg tgccacacgg
     2281 atccagcatg aactttcctg gtttgacggg ttatagcggt cccagttaaa tttcacccgc
     2341 acgcggccat gttcatcaca gaagatttcc tcgcctgccg gtcccgtcac gacggcgctc
     2401 tgcgggccgt ccaccagcgg tttcagcaac ggctgtggcc gccatgtccg gtctgccggg
     2461 attaccgcaa agtggttatc cagcgtggtg cctgaacccc tgcgtcccgg taccgcctgc
     2521 ggctgttcgc cgtgaagatc gctcgccacc acctgccatt cccggttcag gttcgcctgc
     2581 ggatgccccg tcagcacaat tcgccgtccc ggccatatct ccggcgaacg gcttgttccg
     2641 cgcgccactt ctgcgttgtt gcgccagcca tccatctgcc agcgggcaaa gttctgcccg
     2701 tgggcaccct tgaaacgtcc ggggtagtca tacacttcat actgtgtgcg ctggtaatcc
     2761 tggtactggc cttcctgatc aaaacgccct gcccagccgg gtcgtttaaa ggtgtagtct
     2821 ttggtcacca cggaagaagg gcggatttgt gcgctgtagc gaaactggct gatgcagagg
     2881 gtgctcacct cggtacgggt gttcggattc caggggatct caaaggactc cggcagataa
     2941 cgcacggtat cgcacaggac caggctctgg tcggtacttt tttgcgcgtg ctcctcataa
     3001 aagaagatgc cttcctccgc cgccatccgg cacaggaaat cgtaatcagt ctcaccgtac
     3061 tggacacaaa actcacggga aggatgtggc tcgctgaaca gcgggctcca ctcggtcacc
     3121 ccgttttcct gcaatatcgt gccgaggatg ctttcgatgt cctcattctg gaagatacgg
     3181 aagttctggc gcagccctgt gcgccacagc ggtgggcaca ccttcatgct gtacagcatc
     3241 tggtttttgt cgttctcccc cagttcaaac caggtcacca tacctttcac ccggcgctgt
     3301 acgtcatcgc cctgccatat cgtcaggtag gccattttgt cgaggatctg ctggaattca
     3361 agggagagaa actgctggct gaccagagag agatcgaggg aaaaaagcga agagagtgac
     3421 tggttcagat gaaaggagac caccgcaaaa gcatccggtg gcaggccgtc cacttccagc
     3481 gtgaaacgta atccggttga catagcaacc tccggggtta aggaaaaaca gacagttcgc
     3541 tgttaaagcg gctgtgacag tacagtgtta cgcttgcgtt caggcacgct accgccgaag
     3601 tttccggcag ccgccatctc ccgtgaacgt aaccccttac tgacaacctt tctttctacg
     3661 gcgtaataca aaaacagcat ccggcctgat agccggatgc tgttggctta acttatgctt
     3721 ccagcggtgc gcgccagtcg tcagcaccgg aggtgccaga tttgacgtgt tcccattcaa
     3781 ttttgcggta ggccagtgaa acttcgagca actgggtgaa ctcgcgtttc gccgggtcct
     3841 ggcaatgcgg catatgcagt ttcatatcga caatcgtcga atcagtcagg cgggtagtga
     3901 aataatgctc ctgtttgcct tccacagagg tacgccacca gtgaagttca gtggttggca
     3961 gcatttcgcc ggaggccagt gcgttgtaca tcagcggaac cgctttgttc agcgccacgg
     4021 taaaaatgaa aggcttgtga gcacgctgac ccgcaggctg accggactga ggatcggtcg
     4081 ggacagtaac gttatgcagg aattcctgca ccagcatctc gtcttcgtgc ccctgcacat
     4141 agatgttgcc gacagaatca gcggtaaatg cacctgcagt aatatttccc tgggtctgac
     4201 cagtgatgga gatataacac ggtgttggca tttgtaaact ccttgttaac gtggttaatt
     4261 actacttaca atgacctgcc gttaatccca gacaggcaga tgcagctcac ttcacggaac
     4321 cgcatcattt ttcagtattt tttacataca aaccgagcta gaatttacgg ttgtcgtaaa
     4381 aactattttt aaaatcatta aatctcttat tcagcaataa cttaataata tatatctgga
     4441 taaccatacc caactgttca aagtccgtat tgcctccata aaatggccaa agctgaatca
     4501 tgaataaata ttgtatttat cctttctgaa ttatcatttt gataattttt gagatattcc
     4561 ttaaaaatac ccacttaatc aatcacaaaa caaatatata aataagcaat cattagatta
     4621 gcttatgttt aatgaaatta ttatataatt caataagatg cataaaaact ctatatttaa
     4681 attattgcca taatacacct gacgcgaatt gaacaggttc atacaatcta tttaacgctt
     4741 gcagaatacc cctctttacg atcgataaaa agtccttttc ttatttattt tgaagcgtaa
     4801 aatgcggggt gatgaaggag tggtgatttt attttcaccg ccatcaaggc ggcatttccg
     4861 atattttttg cggtacgaat atgccgtgtt catggcctgt acgagataac atgacggatc
     4921 acttttatga gcaaaaaatt tgaaggcagc gtcgcgcctc gcgagcgtat taatatatcg
     4981 tatgtcccaa aaacagatgg ccagacggct gaagtggaac ttccgcttaa catgctcgtt
     5041 gtcggcgata ccggaaatac gcaggaaaca tcctcactgg atgaacgtca ggcagtttcc
     5101 gttaacaagc acaatttcgg tgcagtgatg gctgaagcag ccattggcct caattttacc
     5161 gtccccgcca cgctgaaggg aagcacaacg gacgatgagc tgaatgtggc gctgaatatc
     5221 aagtcactgg atgatttttc accagatagc gtggcccggc aggttccgga agtgaataaa
     5281 ttgctggaac tgcgtgaagc actgactgca ctgaaggggc cgatgggtaa ccttccggca
     5341 ttccgtaccc agctccaggc attgctggaa aatgaagagt cccgcgagca gttgctcaaa
     5401 gaaatcggcc aggtgagcaa taaataacca ccactatttc attaaacagg gttatccagg
     5461 catgacggca cacaaaaata tctccgggac ataccacctc tctgttgcag acattctgca
     5521 ggtggtttac caggtatgtt tcagcccgtc cgttgaaatc aaccaggatg gtgtggctgc
     5581 gctgattaca acactggatc gccgtatcag tgatctattg gatgagatta tccatttttg
     5641 tgagtttcag cagagcgcgt cgcactggca gcgcgtactt cactgacggc gaaaagccgg
     5701 tttcagaaac acagctaaaa cagaaggatt ttcagtatgt ctttacagga agaggaactc
     5761 gtatccagtc atgccgggca gcctgagcag gcatcgtcgc tgcttgatca aatcatggca
     5821 cagacccgta ttcagcccgg ttcggaaggt tatgacgttg cccgccaggg tgtgacagcc
     5881 tttattgcca gcatcctgca atccactgca tctgccgaac cggtcaataa actggccgtg
     5941 gacagcatga ttgcagatat tgatgaacgc atcagccgcc agatggatgt catcatccat
     6001 gcgcctgcct ttcagcaggt cgagtcgttc tggcgctcac tgaaaaccat ggtggatcgc
     6061 gttgatttcc gtgaaaacat caaggtcaac gtgcttcatg tcaccaagca ggaacttctg
     6121 gaagactttg agtttgcgcc agaaattatt cagtccggat tttataagca cgtttattcg
     6181 tccggtttcg gacagtttgg cggtgaaccc atcgctgccg tgctgggcgc ttatgagttc
     6241 aaaaataccg cgcctgacat gaaactgctg caatacgtca gtgccgtggg cgcaatggcg
     6301 cacgcaccgt tcctgtcctc cgtttccccg gagtttatgg ggctgaactc gtggaccgaa
     6361 ctgcctaata tcaaagatct ttatgccatc tttgaaggtc cggcctacac caaatggcgc
     6421 gccctgcgtg actcggaaga ttcgcgctat ttagggctta cggcaccccg cttcctgctt
     6481 cgccagccct attcaccaac agataaccct gttaagaact ttaactatta cgaagatgtc
     6541 agccagaacc acgaagatta tctgtggggt aatacggcct ggatgctggc atgcaatatc
     6601 gcagacagtt ttgccaaata ccgctggtgt ccaaacatta tcggtccgca aagcggcggc
     6661 gcagtgaaag atctgccggt gcatctgttc gaaacgatgg ggcagattca ggccaaaatc
     6721 ccaaccgagg tgctggtcac cgatcgccgc gaatttgaac tggctgaaga gggttttatc
     6781 acccttacca tgcgtaaaga ctctgataat gcagcctttt tctctgcaaa ctcggtacaa
     6841 aaaccgaagc actttccggg aaaagatgca gaaaccaact ataagctggg tacgcagctt
     6901 ccttatctct tcatcatcaa tcggttagcg cactacatca aggtgttgca gcgtgaacaa
     6961 ctggggtcat ggaaagaaag gagcgactta gaaagagagc ttaatacctg gatccgtcag
     7021 tatgttgccg accaggaaaa cccgcctgca gacgtgcgca gccgcaaacc tctgcgcgct
     7081 gccaaagttg aggttatgga tgtagaaggc gaacccggct ggtaccaggt tgcgctaagc
     7141 gtgagacctc attttaaatt tatgggggca aattttgagc tttccctggt tggccggtta
     7201 gacagggagt aacacaccat gataccggat cgggaaagaa ccacaggttc tctttttgag
     7261 cgtatggaag cctcttcggc cagaaaccgg cagggaggca gtattcattc gctcaggcaa
     7321 tccattcgcc agaacctacg aaacattctt aatacccggt ccggtagttg ccggggtgcg
     7381 cctgagctgg gcattgatga accggaaggg gctgaaaact tccgcgaatc catgagtcgg
     7441 gccatcgagc aatgtataga acgctatgaa ccccggatct cacacgctga agtgcaggca
     7501 gtggtttcat ccgcctccag cccgctggat atgacgtttc atattacagc ctgggtgaca
     7561 tttaacgaaa cgcatgaggt gcttgaattt gatatggcac cgaatggcag ccagcattac
     7621 cgggtggact gacaaatgga atttgaagaa cgctatttcc gggaagaact cgattacctg
     7681 cgccagctta gcaagctgct ggcaacggaa aaaccccatc tggcccgctt cctggccgaa
     7741 aaagatgcgg atccggatat tgaacgcctg ctggaagggg tggcttttct taccggcaat
     7801 ctccgccaga aaattgagga tgaattccca gaactgacgc acgggcttat taagatgcta
     7861 tggcctaatt acctgcgtcc ggttccggca atgaccctta ttgaatatac gccggatatg
     7921 gataagtctt ctgtaccggt gttaatcccc cgtaatgagc agtttacaac caacgccggg
     7981 gaaatcagag ttgatgaagt gctgccctct gatgctaaaa aggaggagcc gcctccctgt
     8041 accttcaccc tctgccggga tatctggctg ctgcccgttc gcctggagca gattgaaaac
     8101 cgcagtacga cccgtaatgg tgttatcaac atcacctttt cggtcgcacc gggaacggac
     8161 ttccgcacgc tggatctgaa caaacttcgc ttctggctcg gcaatgacga caactatacc
     8221 cgtgaccagc tttatttatg gttctgcgaa tacttgcagg gtgccgacct gactgtgggt
     8281 gaacagcata ttcgcctgcc tgagtttatg ctaaaagctg tcggttttga gccgcaggat
     8341 gccatgctgc cctggccgaa aaacgtccac agcggctacc ggatccttca ggagtatttc
     8401 tgttaccccg atgcgtttct cttttttgat ctttgtggtt gtccggcttt gcctgacgga
     8461 ttgcaggcgg aattctttac cctgcaactg cgtttttcgc gccctttgcc cgtggacatc
     8521 cggctgcgcc gcgattccct gcgcctgtat tgcgcacctg ccattaattt atttatccac
     8581 catgcagaag ccatcacgct ggacaaccgg cgggcagact atccgctggt tcccagccgc
     8641 cattacccac aacattacga tgtattttcc gttaacagtg tggtgagcca ggtccaggat
     8701 atgttcagga aaaaagatct ggggcgtcct gtttcgacgc aggccgcgcg ccagtggcca
     8761 gcctttgaaa gtttcagcca tcagatggaa tacagccgga agcgggaagt ggtgtactgg
     8821 catcaccgga ccaaaacatc cctgttccat cgcggctttg atcataccct tgcctttata
     8881 catgctgatg gcagttatcc gtcagacgaa tctctgctca gtaatgaagt ggtttcggta
     8941 tcgctgacct gtaccaaccg tgagcttccg tcacaaattc gttccggcga tatcaccggc
     9001 acaaccggta aaaatgcggc tgttgcttca tttcgcaaca ttacccgccc gacgcaacca
     9061 ctctggccgg tcattgatgg cagcctgcac tggtccctac tctccgccat gaacctgaat
     9121 tatctgtcat tactggatac ggacgcgctg aagcaggtca tcgccaactt tgatcgccac
     9181 gcaatccatc atccgcagac ggcacggctg tcacaacaaa agctggatgc cattgagcgt
     9241 ctggagaccc gccccgttga tcgcctgttt acgggtattc ccgtccgggg actggcctcc
     9301 acgctgtatc tgcacccgga gccgtttgtc tgtgaagggg aaatgtatct gctcggtacg
     9361 gtgctttcgc attttctgtc gctgtacgcc agcgttaact cattccacat gctgaccgtt
     9421 gtgaacacag aaagccagga gacatggaaa tggacggaaa gaatcgggca gcatcctctt
     9481 atctgagtcc gggaaatccc ccggcagata aagagcagaa tgatccgctg gcacaggttt
     9541 tccataatgc ctgctcttac aatttttttg cgatggcgga gctgttgcac cgcctggcaa
     9601 agggtgaaaa ggggacgcca gaattatccc tgcgtgacga tccggcacag gaaaccctgc
     9661 gtttcagcgc cgatgccagc cttgccttcc cgtgcagtga tatcagtgcg ctgaaacggg
     9721 atacgtcagg ggcattcagg atgaccacca cctttatggg gttacagggg agccagtcgc
     9781 ctctccccgg ttattacctc gatcacctgg cctggaaagc ggtacatgaa caaagtccgg
     9841 taggtgattt tctggatatg ttcagccacc gcctgaccca gtttgtctgg catatctggc
     9901 gtaagtaccg ctatcacatc agttttcgta acggcggtgt ggatgcgttc tcgcagcgca
     9961 tgtactccct tgtcgggctg ggccaccgcc agcttcgcga taagctggcg attaatcaca
    10021 gcaaaatgct ggcgtattcc gggatcctgg caaacccggg gcgatccccg gaaattatct
    10081 gtggcctggt gtcccactgc tttgatttgt cagaagtgac gctgcaaaac tggcagcgtc
    10141 gcaaggtgga tattgaaccg gatcagcaaa atagtctggg gagctattca cttaaaaatg
    10201 gcgaaaaact ggcgggaaga tcggtactgg gcaactttgt tctgggtaca cgggtgcccg
    10261 atctcagcgg aaaattccag ctcagtatta ccagcctgac ccgaaaacag ttcctctctt
    10321 ttctgccgtc cggcgaaaac ttcctgccac tgacgatgtt tgtgtccttc atcctgcgcg
    10381 atcagcttgc ctgggactta catctggggc tggcgccgga gcaggtgggc gcaatgcgcc
    10441 tgggcgataa caaaagtgcg ctgctgggct ggaccagctt cctcggcaca ccggaagaac
    10501 gaccctcagt caccatcagg gtacggtcgt aaattatgat aatcacggag cagaatatgc
    10561 cggaagagaa actacagacg ctttcattgc aggtcattaa cggcagtgag ctggaaagcg
    10621 ggcgggcggc gcgctgtctg ttcacacagc agggaaatgt cggccatgcc cccgaatgcc
    10681 actggtcggt acaggatcgt cagcatagca ttccggccca ggcttttacc attatcctgc
    10741 acgatggcac attttgtctg cgcccgcaga cggtacaact gtggctgaac caggcaaaag
    10801 tcacagcaac atcagacctg atacagctgc gccagggcga tgagatccag atcgggcgac
    10861 tgatgatgag ggttcatctg aaccggggag atatccccca ttacgatgag gaaatggcga
    10921 cgcccgaaac catcgtcacc aatcgcgata tgctcacgga taccctgctg tcaacggagg
    10981 gtgcgccaca ttatccggga atgactcacc ggcaccagct tgcagacacc gtggtaaacg
    11041 gtttttctgc cgatccactc caggcacttc agtccgaaag cctgattacc acaggcgatc
    11101 cgctttcagg cattgcggct gtccggccct cggcaccgct gtccgatccc gcaagtaatg
    11161 gcgggatcaa ttcgccgttt atggatctgc cgcccattta tgccagcccc ggcgatcgta
    11221 atgatgacgt ctctgcggca gaaatggcgc aacgccacct tgcggtcacc cccttactgc
    11281 gcggtctcgg cggctcgctt accgtgagca attccgacga tgcggatgat tttctggagg
    11341 aggccggacg aaccttacag gccgcaataa aaggtctgct cgatttgcag cagcagcgta
    11401 acagcctctc agacaaacat ttgcgcccgc tggaagataa cccgctgcgc ctgaacatgg
    11461 attacgccac cgcgctcgac gtgatgtttg ccgaaggcaa aagcccggta catctggcgg
    11521 cccccgccgc cgtcagtgaa agcctgcgca atgtccgcca ccacgaagaa gctaaccggg
    11581 cggcgattgt ggagtcactt cgtgtcctgc tggacgcctt ctcacctaaa aatctgctgc
    11641 gccgctttgt gcagtaccgc cgtagccatg aactgcgcca gccgctggat gatgccggag
    11701 catggcaaat gtacagccat tattacgaag aactggcctc cgatcgccag caggggtttg
    11761 agatgctgtt taacgaggtc tacgcccagg tctatgaccg ggtgcttcgt gaaaaacagc
    11821 gggagccgga agcatgaaca ggaaggcgtt tctggcctgc gtgctgatgt gcgtcatatt
    11881 aaccggctgc gaaacggcga aaaaaatcag tgaggtgatc aaaaacccgg atatccaggt
    11941 cgggagcctg aaggagcaac cttcggaaat aaccgtcacc ctgctgaccg aaccggacac
    12001 caataccaat gcggaaggtg aatcagccgc cgtggatgtg caactggtgt acctgactga
    12061 cgactcaaaa ttgctggcag ccgactatga ccagatagcc agtacgccgc taccggatgt
    12121 gctgggtaaa aactatatcg accaccagga tttcaacctg ttgccggaca ccatcaaaac
    12181 cctgccgccg gtaaagctgg atgagaaaac gcagtttatt ggtgtcgtgg cctatttctc
    12241 cgatgaccag gccactgaat ggaaacaaat cgagacggtg gaaggaaccg gccaccatta
    12301 ccgcctgctg gtgcatgtac gtcaaagcag catcgaaatg aaaaaagagg atgaataatt
    12361 atggcaacca cccgcaacaa ggtgatgtgg caggaaggga tgctgatgcg cccacaccat
    12421 ttccagcaac agcagcgtta caacgactac ctggataacc agcgtttccg ggccatgaat
    12481 gatttatcct ggggatttac cgaactcacc ctcaacaatg aactgctggc gcagggtaag
    12541 atcatgattg acagcgcgtc aggcacactg cccgacggca ccgtcttttc tatccccgac
    12601 caggacgcac tgcccgatcc gctgcacccg caacattttc cggacgagag aagccgcaat
    12661 atctacctcg ctctgccggt cgccagtgat gtgagaaatg aaatcagcga cgggcggcga
    12721 atcgggcgtt accggctgaa ttatgccgat gtccgggatt tgcattcaga agaaggtgac
    12781 gcgcgaacac tgacgctggg acaactgacg ccgcgcatta tgagcggtgc agaagatatg
    12841 agcgcctata ttacgctccc gctttgccgt atcagcgatc gccatgccga cggctccctg
    12901 acgctggatg acgattttat cccctcctgc cagaatattc aggtcagtaa gaaactgcgt
    12961 gtttatctca aagaggtaca gggggccatt ggcggacggg caagcgatct ggcaaaccgt
    13021 attggctctc cggcgcagag cggcatcgcg gatgtggcgg aatttatgat gttgcagttg
    13081 cttaaccgta accagacccg gtttacccat cgcgcccgcc gatcccagct ccacccggaa
    13141 gatttctatc ttgatcttgc cgggttgctg ggtgaactga tgacctttac agagctgtcg
    13201 cgcctgccct gcccgcttga tgtgtatgat catcatgacc tgaccaaaac atttaaaaca
    13261 ctgttaccgg aagtcaaacg agcgctgcat accgtactgt cccccagagc cgtcaatctg
    13321 ccgctgcatc tgcgtgacgg tatctggcag gccgatatcc atgacacaga actgctgcaa
    13381 tctgccacct ttgtgctggc cgtggcggca aacatgccgg tcgatcagat ccagcgccag
    13441 tttatccagc agtcgaaaat ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag
    13501 atcccgggta ttcctctgcg cgcactgatg gtggcccccc gccagcttcc ctaccattcc
    13561 gggttcagct atttcgaact cgacaagagc ggccaggcct ggacagaaat ggctgccgcc
    13621 ggagccgttg cactgcatgt ctccggcagt ttcccggatc tgaacatgca actgtgggcg
    13681 ataagagggt aagacgatgg atgaggggtc actgtctctg ccaccattta ccggatacga
    13741 cgaaaaaagc ctgcgcgact accatctcgc gcttcgtggc aacagcctga atccgatgat
    13801 cgacgcggcc acgccgctcc tcggtatggt gatgcgcctt tccactatga acagccagac
    13861 gatgccggag catctttttg ctcaggtggt gacggatgtt caggccgtgg agcaactgct
    13921 tcaggagcag ggctacgaac cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt
    13981 tattgatgaa gcggcgctcg gtaacggctg gtcgaacaag aacgagtgga tcaagcagtc
    14041 attgctggtc catttccaca acgaagcctg gggtggcgag aaagttttca tcctgctgga
    14101 gcgcctgatc cgtgaaccca aacgttacca ggatctgctg gaatttctct ggctctgctt
    14161 ttctctggga tttcgcgggc gctacaaagt ggcagcacag gagcaggggg aatttgagca
    14221 gatctaccgc cgcctgtacc acgttctgca taaactgcgc ggcaatgccc ctttcccgct
    14281 gttacaccag gataaaaaaa cccagggcgg gcgttatcag ctcatcagtc gcctgaccat
    14341 taaacatatt ttttgtggtg gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt
    14401 gcggctggac agccagacgc aggacatcct gtaccagcta aacaaattac ttcgataagg
    14461 acgtttatga tccagattga tcttcccacg ctggtaaaac ggctgaacct gttctcccgc
    14521 caggcgctgg agatggccgc ctctgaatgt atgagccagc aggcagcgga aattaccgtc
    14581 agccatgtgc ttattcagat gctcgccatg ccacgcagtg acctgcgggt tattacccgc
    14641 cagggtgata ttggcatgga agaactgcgc caggcgctga cggtggagaa ctacacaacc
    14701 gcccgttctg cggacagcta cccggcgttt tccccgatgc tggttgagtg gctgaaagag
    14761 ggctggctgc tggcgtcggc tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg
    14821 ctggccctgc tgcattcacc gctgcgttac ataccgcctg ctgccgccca gctgttgacc
    14881 ggcattaacc gtgaccgtct gcaacaggac tttgtgcagt ggacacagga gtcggcggaa
    14941 tcagtcgtgc cggatgcaga ctgtaaaggc gcaggcgcac tgacggacgt cgctgacagc
    15001 ctgcttgccc gctacgccaa aaacatgacc gaagatgccc gtaacgacag gctcgacccg
    15061 gtactgtgcc gcgaccacga aatcgacctg atgatcgaca ttctctgccg tcgccgtaaa
    15121 aataacccgg tggtggtggg cgaagcgggt gtgggtaaaa gcgccctgat tgaagggctg
    15181 gcgctgcgta tcgtggcagg ccaggtgccg gacaagctga aaaacaccga tatcatgacc
    15241 cttgacctgg gcgcattgca ggccggggca tcggtgaaag gtgagtttga gaaacgcttc
    15301 aaagggctga tggcggaggt catttcctcc ccggtgccgg tcattctgtt tatcgacgaa
    15361 gcgcataccc ttattggcgc gggcaaccag cagggtgggc tggatatctc caacctgctc
    15421 aagccggcgc tggcgcgcgg cgagctgaaa accatcgccg ccaccacctg gagcgagtac
    15481 aaaaaatact tcgaaaaaga tgccgccctg tcgcgccgat tccagttggt gaaggtcagc
    15541 gaacccaacg ctgccgaagc cactattatt ctgcgtggtc tgtcggcggt ctatgaacag
    15601 tctcacggtg tgctgattga cgatgacgcc ttgcaggccg cagcaacact gagcgagcgt
    15661 tatctctccg ggcgtcagtt gccggacaaa gccattgatg tgctggatac tgcctgcgcc
    15721 cgcgtggcaa tcaacctttc atcgccacca aagcaaatct cggcgctgac caccctgagt
    15781 caccagcagg aggcggaaat tcgccagctt gagcgcgagc ttcgcatcgg gctgcgtacc
    15841 aacacctcgc gaatgacgga ggtgctggtg cagtatgatg aaacgctgac ggcgctggat
    15901 gaactggagg ccgcctggca tcagcaacag acgctggtgc aggagattat cgccctgcgc
    15961 cagcagttac tgggtatggc agaagacgat gcggcgtcgt tgccgcacgt ggatgccgtg
    16021 gaggatacgc cgccagagtc agaacaggat aataccggtg ccgaaccggc tgatgaagct
    16081 ggcagcgaac agccggaaga gaccgctgaa acagtttccc cggtacaacg tctggcacat
    16141 ctcactgccg aactggacgc cctgcataac gaccggttgc tggtttcccc gcacgtcgat
    16201 aaaaaacaga ttgcggcggt gattgccgaa tggaccggcg tgccgcttaa ccgcctgtcg
    16261 cagaatgaaa tgtcggtcat caccgacctg ccaaaatggc tcggcgacac catcaaaggc
    16321 caggacctgg cgattgccag cctgcacaaa cacctgctga ccgcacgcgc cgacctgcgc
    16381 cgtccgggac gcccgctggg cgcgttcctg ctggctggcc ccagcggcgt gggtaaaact
    16441 gaaaccgtcc tgcaactggc agagctgctc tacggcggtc gccagtacct gaccaccatc
    16501 aatatgtccg agttccagga gaaacatacc gtctcgcgac tgattggctc ccctccgggc
    16561 tacgttggct acggtgaagg cggcgtactg accgaagcga ttcgccagaa accctactcg
    16621 gtagtactgc tcgatgaagt ggaaaaagcg cacccggatg tgctcaacct gttctaccag
    16681 gcgttcgata agggcgaaat ggcagacggt gaaggccgcc tgattgactg caaaaatatc
    16741 gtcttcttcc tgacgtccaa cctcggctac caggtaatag tcgagcatgc cgatgacccg
    16801 gaaaccatgc aggaagtact gtatccggtg ctggccgact tcttcaaacc tgccctgctg
    16861 gcgcgtatgg aagtggtgcc gtacctgccg ctgtcgaaag agacgctcgc caccatcatt
    16921 gccgggaaac tggcccgcct ggataacgtg ctgcgcagcc gctttggcgc ggaggtgatt
    16981 atagaaccgg aagtgacgga cgaaatcatg agccgcgtca cccgcgcgga aaacggcgcg
    17041 aggatgctgg aatcggtcat cgacggcaat atgctgccgc cgctctccct gctgctgttg
    17101 cagaaaatgg cggcgaatac ggcggttgcc cggattcggt tgtcggcagt ggacggcgca
    17161 tttacggcag acgtggaaga tgctcagaac gacgagtccg tcacaaagga tgaaacggtt
    17221 ttatgagcag gttgcgcgtt ttccggcaga caggatggtt tatcgcgggc ctgatgacgg
    17281 gcttacccgc cactgccgcg cccgcagaag catcgtcaat ggccggtgtg gcggtcgccg
    17341 tcgccaccac gaccccgccg gatgcgacag ccacgttaca ggccatgcag tcctgtcggc
    17401 gggagtccgc ggcgctggag cggcttgact gctatgaccg cctgctggca ccgctgtccc
    17461 cgtcagggtt tgacggggcg ctggtcaaag ccggttttgt gggcgaagcc tggacccgcg
    17521 ccacggaaca ggaaaaacac cgccagggca acaccacaga gctgctggtg acgcaggtgc
    17581 cgggggagcg cccgacggtg gtgataacca ccccggctat cggtcatgtt ccgccacgcc
    17641 cggtgctgat gttcagttgc gtggacaata tcacccggat gcaggtggcg ctgatgcacc
    17701 ctctggatgt ccatgatatc gccgtcactc tgaatgccga caaccgggca ttgcgcagcc
    17761 actggtttgt gcgcgagaac ggcaccctgc tggagtccag ccgggggtta tccgggattg
    17821 atgaaattaa gcaactgttc ggcgcaaaaa cgctgacggt ggataccggc gcagacaatg
    17881 ccgccggaaa gctgaccttt aacattgacg ggctggcaag ggcgattgct cccctgcgcg
    17941 atgcctgcca ctgggcggga gaataaaaat atggcaatgg acttacgcga tccgaatgtc
    18001 tggatatcgc acctgctgga aaacctgccg gaagagaaac tggcatcggc gctgaaagat
    18061 gacaacccga actgggagta tatcgacggc gaaatcgtca agctgggatc gctggcccac
    18121 agtcaactgg atatcccgga actccagcgc cgtgggctgg tcattctggc ttcggagagt
    18181 aaggattttc gtctgctggc gcatctgctg agaaccctgc aacacgccgg tgatcctcat
    18241 ctcgcattgc gcctgctggc gctgtatgtg gagcattact gggcggtggc tgcgccgcag
    18301 aatatggcgc ataaaaaacg ctttgccagc caggtcatta aacgctttga aacaggtatt
    18361 gaagtttttt cacagaacgc cgccacagcg cagcgcgatg cgctgtcagg ggaactggca
    18421 aaactggcgc aatgctggca gtcacataac gccccggaac tggcacaggc tactgatgat
    18481 ctttttgccc tgtaccagcg tgcgtttaat cgtgcggcac cggctcctgt ccccactccg
    18541 gcggcctccg gtagttcacc acagaccacc gccacgtctg aaagcggcgt gacgcaaccc
    18601 agtgctccgg ctccccaaat cgtcatcgac agtcacgacg acaaagcctg gcgcgacacg
    18661 ctgttaaaag tggcggccat tttatgtgaa cgccagccgg attcaccgca gggctaccgt
    18721 ctgcgccgcc atgcgctttg gcagaacatc accagtacgc cccaggcgga aagcgatggc
    18781 cgtactccac tggctgcggt ctccgccgat atggtggcag attaccatgc tcagctcggt
    18841 agtgccgata tggcgctgtg gcagcaggtg gagaaaagcg tactgctggc tccttactgg
    18901 ctggatggtc actgcctttc tgcacagacg gcgctgcgtc tgggttacaa acaggtggca
    18961 gacgccatcc gcgatgaggt catccgcttc cttgagcgtc tgccccagct taccgggctg
    19021 ctgtttaatg accacacacc gtttatcagt gagcagacga aacaatggct ggcagcttcg
    19081 cccgacgcca aagttgcacc ggttgcgcaa atcggtgagg aatcgaaggc agccagagcc
    19141 tgttttgctg agcagggtct ggaggcggcg ctgcgatatc tggacatgct acccgaaggc
    19201 gatccccgcg atcagtttca ccgccagtac cttgccgcac agttgacgga agaggcgggg
    19261 ctggtacagc ttgcgcagca gcagtaccgg atgttgttca ggatgggatt gcagatgatg
    19321 gtggcagact gggagccgtc cctgcttgag cagctagaac aaaaattcac ggcagaacaa
    19381 taaaagagaa caggagtttt tgtgttcaga ttaccaacac cccgactact cagcggactc
    19441 aaatcagccc tgcgaccggc gatgccccgg tttaaagtct ctgctttctg gctgctgata
    19501 ctggcgtgga tttttctgct ggtgtggatc tggtggaaag gcccgacgtg gacgctgtat
    19561 gaagaacagt ggctcaaacc actggcgaac cgctggctgg caacggcggc gtgggggatt
    19621 attgccctga tgtggcttac cgtccgggtg atgaagcgcc tgcaacagct tgaaaaaatg
    19681 caaaagcaac agcgcgagga agccgttgat ccgctcagcg tggaactgaa cgcccagcag
    19741 cgttatcttg accgctggct gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg
    19801 tggcagttgc cgtggtatat ggtcatcggc ccggcgggca gtggcaaaac gacactgctg
    19861 cgcgaggggt ttccgtccga cattatttat gccccggagg gcgcacgcgg cgcagaacaa
    19921 cgcctgtacc tcacgcccca tgtcggtaaa caggcggtga tctttgatat cgacggcaca
    19981 ctctgcgctc ccgctgatgc ggatatcctg catcgccgtc tgtgggaaca tgccctcggc
    20041 tggctgaaag aaaagcgcgc gcgccagccg ctgaatggga ttattctgac actcgattta
    20101 cccgatctgc ttaccgcaga caaacgccgc cgcgagcatc tgttacaggc gctgcgcagc
    20161 cgcttgcagg atatacgcca gcatcttcac tgccagttac cggtttacgt ggtacttacc
    20221 cggcttgatt tattgcaggg ttttgccgcc ctgtttcagt ccctgaacag acaggatcgc
    20281 gatgcgattc tgggcgtcac gttcacccgc cgtgcccatg aaaatgatga ctggcgaaca
    20341 gagttgaatg ctttctggca gacatgggtg gatcgaatga atctggcgtt gccggatctg
    20401 atggtcgctc agactcacac ccgcgcgtct ttattcagtt tttcccgcca gatgcaggga
    20461 agccgtgaac cgctggtgtc actgcttgag ggtctgcttg atggcgaaaa tatgaacgtg
    20521 atgctgcgtg gtgtctatct cacctcttcg cttcagcgtg gacagatgga tgatatattc
    20581 acccagtctg ccgcccgcca gtaccggctg ggcaataacc cactggcgtc ctggcccctg
    20641 gtggacaccg cgccttattt cacccgcagc ctgttcccgc aggcattact cgcagagcct
    20701 aatctggcaa cagagagccg cgcctggctg atacgttccc gtcgccgcct gacggttttc
    20761 tctgccacag gcggcgtggc agcactgctg ctcatcaccg gctggcatca ctattacaac
    20821 ggtaactatc agtctggcat caccgtgctt aagcaggcca aagcctttat ggacgtgccg
    20881 cctccgcagg gggaagatga ctttggcaac ctgcaactgc cgctcctgaa cccggtacgc
    20941 gatgccacac tggcctatgg cgactggggc gaccgcagcc gtctggccga tatgggactg
    21001 taccagggac gacgtatcgg gccttatgtg gaacagacct atctgcaact gctggagcaa
    21061 cgttacctgc cctcgctgtt taacgggctg gtcaaagcgc tgaacgccgc gccgccggag
    21121 agtgaagaaa aactcgcggt gctgcgcgtg atgcgaatgc tggaggacaa aagcggacgt
    21181 aacaatcagg tggtgaagca gtatatggca aaacgctgga gcgaaaagtt ccacggtcag
    21241 cgcgatatcc aggcacaact gatgtcccat cttgactacg cgctggctca tactgactgg
    21301 cacgcagagc gtcaggcggg cgacggtgac gccatcagcc gctggacgcc atatgacaag
    21361 cccgtggtat cagcacagaa agaactgagc aaactgcctg tctaccagcg ggtttaccag
    21421 agcctgaaaa cgcgggcgct gggcgttctt cctgccgacc tcaatctgcg tgaccaggta
    21481 gggccaacct ttgaccaggt gtttacatct gccgatgaca acaaactggt tgttccacag
    21541 tttcttaccc gttacggcct gcaaagctat tttgtaaaac agcgcgatga actggttgaa
    21601 ctgacggcga tggattcctg ggtacttaac cttacccgca gcgtgaaata cagtgacgcc
    21661 gaccgcgcgg aaatccagcg ccagttgacc gagcagtata tcagcgacta caccgccacc
    21721 tggcgggccg ggatggacaa tctgaatatc cgcaattttg agtccatcgg acaactgacc
    21781 ggggcgctgg agcaggttat cagcggcgac cagcctttgc agcgggcgct gaccgtgctg
    21841 cgtgacaaca cacagccagg cgtcttttct gaaaaactct ctgccaaaga acgggaggaa
    21901 gccctggcag agccggatta ccagttactc acccgcctcg ggcatgaatt cgccccggaa
    21961 aacagtaccc tggcagtaca gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa
    22021 ctcaccgagt tgcaccgcta cctgctggca atccagaacg cgcctgtacc agggaaatcg
    22081 gcgctgaaag ccgtgcagtt acggcttgat cagaacagca gcgatccgat attcgccacc
    22141 cgccagatgg caaaaacgct gcctgctccg ctcaaccgct gggttggcag actgactgac
    22201 caggcctggc atgtggtgat ggtggaggct gttcattata tggaagtgga ctggcgcgac
    22261 agcgtggtga aaccgtttaa cgagcaactg gcaaataact atccgtttaa tccgcgttct
    22321 gcacaggatg cctcactgga tgccttcgaa cgcttcttta aaccggatgg catactggat
    22381 accttctacc agcagaacct gaagctgttt atcgataatg acctgagtct ggaggatggc
    22441 gataacaacg tcattattcg cgaagatatt attgcgcaac tggaaactgc gcagaaaatc
    22501 cgtgacatct tcttcagcaa acagaacggt ctgggaacat cctttgccgt ggaaacggta
    22561 tcgctttcag gcaataaacg ccgcagtgta ctgaaccttg acggtcagtt agtcgattac
    22621 agccagggcc gtaactatac cgcccatctg gtctggccta acaacatgcg cgaaggcaac
    22681 gaaagtaagc tgacgctcat cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc
    22741 gggccgtggg cgcagttccg cctgttcggg gccggacaac tgaccggagt acaggatggc
    22801 aactttaccg tgcgctttag cgtggacggt ggcgcgatga cctaccgtgt gcataccgac
    22861 acggaagata acccgttcag cggtgggttg ttcagccagt ttggtctgtc agacacactg
    22921 tactgaggag gcagggatga atagtaacgt actgacacaa actatcgtta ccggcagtga
    22981 cccgcgcggg ctgccggaat tcagcgccat ccgcgaggaa ataaacaaag ccagccaccc
    23041 gtcacagcct gagctgaact ggaaactggt ggagtcgctg gcactggcga tttttaaagc
    23101 caacggtgtg gatttacaca ccgccaccta ctatacgctt gcccggacac ggacacaggg
    23161 actggcggga ttctgcgaag gtgcggaact gctggcggca atggtaagcc acgactggga
    23221 taagttctgg ccgcagggcg gcccggcgcg tactgaaatg ctggactggt ttaactcccg
    23281 aaccggcaat attctgcgtc agcaaatctc ctttgcggaa tccgacctac cactgatcta
    23341 ccgcacagag cgggcattgc agcttatctg cgataagctc cagcaggtgg aactgaagcg
    23401 cgttccgcgc gtggagaatc tgctctattt tatgcagaac acgcgtaaac ggcttgaacc
    23461 ccagctgaag agtaacactg agaacgccgc acagaccacg gtcagaacgc tgatttatgc
    23521 cccggaaaca caggcatctt ccacaccaga agcggtagtg cctcccctgc ccggcctgcc
    23581 tgagatgaaa gtggaagtgc gcagtctgac agagaatccc ccacaggcca gtgtgataaa
    23641 gcaaggcagt acggtaagag ggtttatcgc agggatcgcc tgttcagtgg ctgtcgcctc
    23701 agcattgtgg tggtggcagg tctatccggt acagcagcaa ctgttacagg ttaacgacac
    23761 cgctcagggc gcagcaacgg tgtggatggc ctcacctgaa ctcgaaaact atgagcgcag
    23821 gctgcaacaa cttcttgata cctccccggt acagccgctg gaaaccggga tgcagatgat
    23881 gcgtgttgcc gacagtcgct ggccggaaag cctgcaacag caacaggcct cgacacaatg
    23941 gaatgaggca ctcaaaaccc gcgcacagag tagcccgcag ttgcgtggct ggttgcagac
    24001 ccgccaggac ttacatgctt ttgcagatct agtgatgcag cgcgagaaag agggactaac
    24061 cctttcctat atcaaaaatg tcatctggca ggcggagcgg ggactggggc aggaaacacc
    24121 cgttgagtct ctgttgacgc agtaccagga tgcccgtgcg cagaagcaga atacagatgc
    24181 gctggaaaaa caaattaatg agcgactcga aggcgtgtta agccgctggc tgctgctgaa
    24241 gaataacgtc atgccagagg cggcaaccgg taccacggcc gaaaaataat tatttaatta
    24301 aaggagatta tcatggcaaa tataagttat ttatctttga gtggagagac acaagggctg
    24361 atttcagcag gttgctcaac gcttgattct gtcggaaaca aagcgcagcc agaacataaa
    24421 gatcagatca tggtttatgc gttaatgcat tccatctcaa gaagtcaaaa tgtcaatcat
    24481 catgaattaa ttatcaccaa accggttgat aaatcctcac cattgctggc aaaagcactt
    24541 agtgacaatg aaaaaatggc aatatgtgaa tttatactct accgaaccag taaagcgggt
    24601 atttaccagc cttattataa aataaatctg tcaaaagccc gaatatcaag tattgatttt
    24661 gtcacaccgc atgctgtcct ggaaaaagaa ctggaaccac aggagagaat tgcctttatt
    24721 tatgaagata tttcctggga gcatacgtta gccggtacga atgcaatgag taaatggcag
    24781 gatcgtgtac agtaacttac tgtaataaca taataaatta ctattgcgga gagataaaat
    24841 ggctggttta aggaataaca acaacacaca gaatgcacag tgggctgatt atgtgggtga
    24901 cattcttcgg ggggctcagc cgataaacca gctagtacct cagcaccctt atcttaacga
    24961 cgtcccactc attgatgagt taaggcatca gaacactcat catgttattc tcttgacact
    25021 tgatgttgca aaaaaaaata ctcagcccaa taacatcatt tgattacatt cattttatca
    25081 ctacacatcc ttcaggtata aaagatacac tcgcctggtt agttaacgcg agtaaattaa
    25141 tgactgagtt tgatgacaat gggaaaatta tatttaacct taatgcctta aagtatacaa
    25201 aagcaagtta ctttgaaata cttggagaga aatatataaa aatcacaaca tccagcccct
    25261 ggcttttaga aaagctcggt aaatatattt tctcttcacg agcgcctcaa gttctggaac
    25321 tcgctattgg ctggcgagga gctttatctg aatcaataaa aggagttaag ttctgtatct
    25381 ggttttcagt agcctggaga accatagaat ttattatgag ttcagagcga gatctggtta
    25441 attttcttgg cgatttttca atggatgtgg ccaaagcagt tatagccggt ggtgttgcaa
    25501 ccgctattgg aagcctggct tcttttgcct gtgttagctt tggctttcca gtaattcttg
    25561 tcggaggagc aattttactg acagggatag tgtgtacagt tgttttaaat gaaatcgatg
    25621 ctcaatgcca tttatcagaa aaattaaaat atgcaattag agatggacta aaacggcaac
    25681 aggaacttga taaatggaaa agggaaaaca tgactccatt tatgtatgtt cttaacactc
    25741 cacccgtgat atgaggatta accatgcgtt tacatgttaa attaaaagag tttctttcga
    25801 tgttttttat ggccatatta tttttccctg cattcaatgc ttctttgttt tttaccggag
    25861 ttaaacctct atattcaata ataaaatgct ccacggaaat attttatgat tggagaatgt
    25921 tgattctgtg ctttggtttc atgtcgtttt cctttttaaa tgttcatgta attttactta
    25981 cgataataaa atcattctta atcaaaaaaa cgaaggtagt caatttcgcc acagacataa
    26041 caatacagct aactcttata tttttactaa tagctatcgt aatagcacct ttaatagctc
    26101 cttttgtcac aggatatgtc aatgctaatt atcacccctg cggtaataat acggggattt
    26161 tcccaggagc aatctatatt aaaaatggca tgaaatgtaa taatgaatat atttctcgca
    26221 aggaagattc agcagttaaa taattaaata ttctaaaaca aacagttttt aactccggat
    26281 tccatgtcct ttcttgcact gtttttgtca agaaaaattt cttatgcaaa gaattttgta
    26341 ttattgccgc cagaaattcc tggcggcaga tataatttat tattgatagc ggttaaccaa
    26401 atcttttacc atcgctttat aggtcttttg cttaagttgc atgatttgct gctctgtcat
    26461 atcagtcgta ctggcttcat tcttgtcggg caatggtgga agcactttat attcataatt
    26521 caccggcgac caggtagccc actcacctgt tgccacatcc accagcgtga aacgtaccag
    26581 gtagcgcagg gagatagcgt cggtgagttt ttcattttta tagtcggacc atactgttga
    26641 tttcagcgca gagtcatatt ttcctgtctg caatgtatcc tggtaaacaa taatcgcttt
    26701 ctgatgtcct tttgcagcca caaaacgcag tgcctgcatc cagttcatat tctcagcact
    26761 ggcaacatcc tcgttctcat ttttatcttt gtttttgtta caagtcagag gcttttgcct
    26821 gtccgggata ccagagaatg tggaaacagt ataatatttt cgcatctcct cctgcataat
    26881 ggtttccggt gcgcggttgc cggattgaac aagtattaca ggagaattaa ggggaacgcg
    26941 gaactcatct ccatcaagcg cagcttgaat atcctcttcg gatacggcca gagtggtttc
    27001 attaccgaat aaatctttgt ctgtcagttg ctggggagtg tttttattaa tattactttg
    27061 tgtgacttga gcttttttct tcttttcatc aatagaagat tcagaggcac atcctgcaag
    27121 cagcgagaat aacacacata tgcaacaaag cttttttgaa ttcataattg gacactccct
    27181 cgcctgtcat aatcatggta atgtttagct aacgattttt aattatatac tccacttgaa
    27241 tcagaacaaa caaaaataac agtatatata aattcattaa atcaatagaa aaatgaaaat
    27301 cataacattc aaatatattt aaattgaatg attcgatagt atgattaaat attaatgata
    27361 tggtctacca tatgtgatta acacatttac agcataacaa aaccatagac cgaagaaata
    27421 gcgggattta aatgcaaaaa aaaaccgcaa aattaaaaat cttgcggttc tctgaactca
    27481 ttttcatgag tgaatttggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca
    27541 ggcaggtatt ctaaccgact gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg
    27601 aatattacgg attgcctcac ccttcgtcaa cggtttttct catcttttga atcgtttgct
    27661 gcaaaaatcg cccaagccgc tatttttagc gccttttaca ggtatttatg cccgccagag
    27721 gcagcttccg cccttcttct ccaccagatc aagacgggct tcatgagctg caagctcttc
    27781 atctgtcgca aaaacaacgc gtaacttact tgcctgacgt acaatgcgct gaattgttgc
    27841 ttcaccttgt tgctgctgtg tctctccttc catcgcaaaa gccatcgacg tttgaccacc
    27901 ggtcatcgcc agataaactt ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt
    27961 tcgtttactg ttatctattt cgtagcgggc acataacgca tcgaggctgt tgcgcttacc
    28021 aggaaacatt ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg tattggtctt
    28081 cggaatatcg cgcttaagca acgaaaactc gtagtccata aagccgatat cgaacgctgc
    28141 gttatggatc accaactccg cgccgcggat atagtccatg aactcatcgg ctacttcggc
    28201 aaacgtgggc ttatcgagca aaaattcatc ggcaatacca tgtacgccaa aggcttccgg
    28261 atccaccagc cgatcgggtt tgagataaac atggaagtta ttgcccgtca ggcgacggtt
    28321 caccacttca acggcaccaa tctcaatgat cttgtggcct tcatagtgcg caccaatctg
    28381 gttcataccg gtggtttcgg tatcgagaac gatctggcgt gtaattgcag tgctcatagc
    28441 ggtcatttat gtcagacttg tcgttttaca gttcgattca attacaggaa gtctaccaga
    28501 gatgcttaaa caggtagaaa ttttcaccga tggttcgtgt ctgggtaatc ctggacctgg
    28561 gggttacggc gctattttac gctatcgcgg acgcgagaaa acctttagcg caggctacac
    28621 ccgcaccacc aacaaccgta tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa
    28681 agaacattgc gaagtcattt tgagtaccga cagccagtat gtccgccaag gtatcaccca
    28741 gtggatccat aactggaaaa aacgtggctg gaaaacagca gacaaaaaac cagtaaaaaa
    28801 tgtcgatctc tggcaacgtc ttgatgctgc attggggcag catcaaatca aatgggaatg
    28861 ggttaaaggc catgccggac acccggaaaa cgaacgctgt gatgaactgg ctcgtgctgc
    28921 ggcaatgaat cccacactgg aagatacggg ctaccaagtc gaagtttaag cttgtggttt
    28981 acgacattgc cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt tactctgttt
    29041 catcgggttt agcgttaaag gaatagtccg tttccgggca acaataagtt gtaagcaacc
    29101 aagcgcagga atatgcgcat tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg
    29161 gaaacggctg gcgtgcagca cttcaaaatt caacaaagag agccagtcca gttgccgcat
    29221 cagagtaaac atccggctgt tatagggcga ggtttttcgc aataccggca caagtttgcg
    29281 taatcccatt aaactaatgg gattgaagcc actaatcacc agccagccat catcaatcaa
    29341 tacccgatcg gtttcacgca ataaacgatg cggatcggtg caccacggca atgtatgtgc
    29401 cagtagacaa acatcaacgg atttatcggc aaaaggaaga tgaagtggat ccgcctgcac
    29461 ctggacgggc attccttgcg cagaaacatt cacttgatga gaaaccgcgc acgcttcgca
    29521 attgatttct gcgcttaaat tgccaatctt aagcagatga aaaccataca ttttagtgaa
    29581 ccacgggttg agctggcgct ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc
    29641 ccagcgatca ggagccacga cagtttgagg gactcttgcc ggtttcatca caaccttccg
    29701 tttcacacta agaggtaatc tatgaatctt aacagtattc ccgcctttga tgacaattac
    29761 atctgggttt tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg agacgcagag
    29821 ccggtattaa acgccattgc cgccaataac tggcaaccgg aggccatatt tctcacccac
    29881 catcatcacg atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg
    29941 gtgtatggtc cacaagagac acaagataag ggaacaacac aggtagtcaa agatggcgaa
    30001 actgccttcg ttttggggca tgaatttagt gtaattgcca caccgggtca cactttagga
    30061 catatctgtt acttcagtaa accttatcta ttttgcggcg acacgctgtt ttctggtggg
    30121 tgtggtaggt tgtttgaagg gacagcatca caaatgtatc aatcacttaa taagttaagt
    30181 gcgttacctg acgatacatt ggtatgttgt gctcatgaat atacgttatc aaatatgaag
    30241 tttgctttga gtattcttcc gcacgatttg tccataaatg attattatcg taaagttaag
    30301 gagttacggg caaaaaatca aataacacta cccgtaattc tgaaaaatga gcggcaaatt
    30361 aatgtttttt taagaacgga agatcttgat ttaattaatg taattaatga agaaacatta
    30421 ttgcaacaac ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa
    30481 aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg
    30541 acacacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga
    30601 gtaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag
    30661 caaagtttac aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg
    30721 acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc
    30781 gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg
    30841 cagagccgta tatgtactgg atagcagggc aagttaaaaa acgtaacatg cctatggaac
    30901 tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg
    30961 ccgcaggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc
    31021 gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc
    31081 agcgtctgaa caaaatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg
    31141 gcgaaggtcg ggtcatgaag g
//
LOCUS       DAFATA010000047        30507 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFATA010000047 DAFATA010000000
VERSION     DAFATA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.048"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..995)
                     /gene="tcyP"
                     /locus_tag="LGJ20_002582"
     CDS             complement(<1..995)
                     /gene="tcyP"
                     /locus_tag="LGJ20_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBK2386626.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFATFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIA"
     gene            complement(1128..1718)
                     /locus_tag="LGJ20_002583"
     CDS             complement(1128..1718)
                     /locus_tag="LGJ20_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2386627.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(1881..2549)
                     /gene="hxpB"
                     /locus_tag="LGJ20_002584"
     CDS             complement(1881..2549)
                     /gene="hxpB"
                     /locus_tag="LGJ20_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBK2386628.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            2696..3232
                     /locus_tag="LGJ20_002585"
     CDS             2696..3232
                     /locus_tag="LGJ20_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBK2386629.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3273..4133)
                     /locus_tag="LGJ20_002586"
     CDS             complement(3273..4133)
                     /locus_tag="LGJ20_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK2386630.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4239..4529)
                     /gene="ghoS"
                     /locus_tag="LGJ20_002587"
     CDS             complement(4239..4529)
                     /gene="ghoS"
                     /locus_tag="LGJ20_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBK2386631.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(4630..5559)
                     /gene="pfkB"
                     /locus_tag="LGJ20_002588"
     CDS             complement(4630..5559)
                     /gene="pfkB"
                     /locus_tag="LGJ20_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBK2386632.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            5846..6604
                     /locus_tag="LGJ20_002589"
     CDS             5846..6604
                     /locus_tag="LGJ20_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBK2386633.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(6657..6764)
                     /locus_tag="LGJ20_002590"
     CDS             complement(6657..6764)
                     /locus_tag="LGJ20_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386634.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(6940..8838)
                     /gene="espL1"
                     /locus_tag="LGJ20_002591"
                     /pseudo
     CDS             complement(6940..8838)
                     /gene="espL1"
                     /locus_tag="LGJ20_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            9362..11290
                     /gene="thrS"
                     /locus_tag="LGJ20_002592"
     CDS             9362..11290
                     /gene="thrS"
                     /locus_tag="LGJ20_002592"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK2386635.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            11294..11836
                     /gene="infC"
                     /locus_tag="LGJ20_002593"
     CDS             11294..11836
                     /gene="infC"
                     /locus_tag="LGJ20_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK2386636.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            11933..12130
                     /gene="rpmI"
                     /locus_tag="LGJ20_002594"
     CDS             11933..12130
                     /gene="rpmI"
                     /locus_tag="LGJ20_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK2386637.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12183..12539
                     /gene="rplT"
                     /locus_tag="LGJ20_002595"
     CDS             12183..12539
                     /gene="rplT"
                     /locus_tag="LGJ20_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK2386638.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    12656..12780
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            12662..12706
                     /gene="pheM"
                     /locus_tag="LGJ20_002596"
     CDS             12662..12706
                     /gene="pheM"
                     /locus_tag="LGJ20_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBK2386639.1"
                     /translation="MNAAIFRFFFYFST"
     gene            12990..13973
                     /gene="pheS"
                     /locus_tag="LGJ20_002597"
     CDS             12990..13973
                     /gene="pheS"
                     /locus_tag="LGJ20_002597"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK2386640.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            13988..16375
                     /gene="pheT"
                     /locus_tag="LGJ20_002598"
     CDS             13988..16375
                     /gene="pheT"
                     /locus_tag="LGJ20_002598"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK2386641.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            16380..16679
                     /gene="ihfA"
                     /locus_tag="LGJ20_002599"
     CDS             16380..16679
                     /gene="ihfA"
                     /locus_tag="LGJ20_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBK2386642.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            16780..17760
                     /gene="btuC"
                     /locus_tag="LGJ20_002600"
     CDS             16780..17760
                     /gene="btuC"
                     /locus_tag="LGJ20_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBK2386643.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            17823..18374
                     /gene="btuE"
                     /locus_tag="LGJ20_002601"
     CDS             17823..18374
                     /gene="btuE"
                     /locus_tag="LGJ20_002601"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBK2386644.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            18374..19123
                     /gene="btuD"
                     /locus_tag="LGJ20_002602"
     CDS             18374..19123
                     /gene="btuD"
                     /locus_tag="LGJ20_002602"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBK2386645.1"
                     /translation="MSIVMQLQDVAESTRLGPLFGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19201..19665
                     /locus_tag="LGJ20_002603"
     CDS             19201..19665
                     /locus_tag="LGJ20_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2386646.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            19912..20625
                     /gene="ydiV"
                     /locus_tag="LGJ20_002604"
     CDS             19912..20625
                     /gene="ydiV"
                     /locus_tag="LGJ20_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBK2386647.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            20688..22124
                     /locus_tag="LGJ20_002605"
     CDS             20688..22124
                     /locus_tag="LGJ20_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBK2386648.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(22128..22319)
                     /gene="hemP"
                     /locus_tag="LGJ20_002606"
     CDS             complement(22128..22319)
                     /gene="hemP"
                     /locus_tag="LGJ20_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBK2386649.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(22451..23497)
                     /gene="aroH"
                     /locus_tag="LGJ20_002607"
     CDS             complement(22451..23497)
                     /gene="aroH"
                     /locus_tag="LGJ20_002607"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBK2386650.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVAAHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(23654..24487)
                     /gene="ppsR"
                     /locus_tag="LGJ20_002608"
     CDS             complement(23654..24487)
                     /gene="ppsR"
                     /locus_tag="LGJ20_002608"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBK2386651.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            24820..27198
                     /gene="ppsA"
                     /locus_tag="LGJ20_002609"
     CDS             24820..27198
                     /gene="ppsA"
                     /locus_tag="LGJ20_002609"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBK2386652.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(27255..28901)
                     /gene="fadK"
                     /locus_tag="LGJ20_002610"
     CDS             complement(27255..28901)
                     /gene="fadK"
                     /locus_tag="LGJ20_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBK2386653.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(28958..29251)
                     /locus_tag="LGJ20_002611"
     CDS             complement(28958..29251)
                     /locus_tag="LGJ20_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBK2386654.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(29248..>30507)
                     /locus_tag="LGJ20_002612"
     CDS             complement(29248..>30507)
                     /locus_tag="LGJ20_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK2386655.1"
                     /translation="VGAGVAGSVAALVMARAGLDVLVIERGDSAGCKNMTGGRLYAHT
                     LEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHASYTVLRNRLDP
                     WLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILADGVNSMLGRSL
                     GMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSDGLMGGGFLYT
                     NKDSISLGLVCGLGDIAHTQKSVPQMLEDFKQHPAIRPLISGGKLLEYSAHMVPEGGL
                     AMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAAKEREDFSASS
                     LAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMFTIDGKPNQPV
                     RKMIMGHAKKIGLINLLKDGIKGATAL"
BASE COUNT         7715 a         7258 c         7824 g         7710 t
ORIGIN      
        1 ctggcgatgg attcaggaac gcccagacga cgcgtttgtg cttccacatt cagtgggata
       61 gacgcagcac tggaacggct ggtaaaggca aacgtcagca caggccatac cttacggaag
      121 tacttcagcg gactcacgcc attaatgccc agcagaatgc catgcactgc aaacataatc
      181 agcagaccga ggtaggacgc gacaacgaaa cttcccagtt tgatgatgtc ttgcaagtta
      241 gaacctgcaa ccactttggt cattagagcc agaacgccgt aaggggtcaa ctgcatgaca
      301 aggcgaacca gtttcatcac ccagctttgt agggtatcga tagcggctaa gacgcgttca
      361 cctttcggcg catcatcctt cagcagtttc aatgcagcta cgccgaggaa tgtggcaaaa
      421 attaccacgc tgataattga cgtcggattg gctccggtaa gatcggcaaa cgggtttttc
      481 gggataaagg acaagaccag ctgcggaacg ctcagatcag agactttacc aacatagtta
      541 ctttcaatgg cgttcagacg tgcagtttct gcaccaccct gaaccagacc ttcagccgtc
      601 aaaccaaaca ggttggtgac cagcacaccg accagcgccg caatcagcgt ggtaaacaaa
      661 agcgtaccga tggtcagaaa actgattttg cctaactgag atgcgttatg cagacgggca
      721 accgcgctca gaatagaggc gaagactaac ggcataacga tcatttgcag cagttgaaca
      781 tagccgttac caacgatgtt aaaccactgt acagaatctt taagtacctg gctgtcagaa
      841 ccataaatgg tatgcagggc aaggccaaaa accacaccca tcaccagacc caccagcact
      901 tttttcgcca gactccactg tttatggcgg gtctgagcca gcgcaaacag cagtacaacg
      961 aacaccacga tgttcgcaat taatggaaag ttcatccccg ttctcctgat cttattattt
     1021 ctatcactgc gtagcagtca tagtgttggc gcaagattaa cagaactgtg agctggctct
     1081 tatattcaaa tggaattgct tattccagaa aggtgatttt tgccttttta gtattcaacc
     1141 tgatgcttaa taagccgatg aaactggatc tgcaaggtat tgatcatttg cgatgaccaa
     1201 cgaacagcgc tgttctcggg taatgaatgg ggcatccata ccgaccagac aaataatgcc
     1261 atgcagataa aacgttccag ttggttgccc ttttggggaa ccaggatagg caagcggaaa
     1321 cgccagcggc atggccagag caggggaaca ccggcgggtg tcagcatatc ggcaagtatg
     1381 tggctcaaat aacccagcac cattccttgt agcgcatcag ccggaatgaa ccagccttcc
     1441 ggaaccttaa ggtaaaaggt tgccagcagc gcaaataccg ccagcagact gtgggtaaaa
     1501 ccacgatgcc caaaagcgcg ggcgatcggt tttgatatcc attttaatcg ctgcccaaga
     1561 aacgactttg ggtgatcgat gtccggtaac aaacacgtca ggattgcgga agggacaata
     1621 tgccaccagt caccctgtgc cagcacgggc gtcagctcgg catttttggc aaataccgca
     1681 caagcaatag aaaagagaag gtgaccttcc gccgtcatga taaaactcac taaactgtcg
     1741 atacgtacag tataggattt ttatacagtg cacggaagtg gtgacggtgt aactctttct
     1801 cttaaattga cgattaatcc gtcagttgaa tgcaaattca gataaagaac caggggcagc
     1861 gtgatcactg cccctggtca ttaaccgaga aggtcttttg cggtgagttc tgtcagcgat
     1921 gagagtttga cgtttgctaa tacaaaacgt ggatcatttt gcgcttctgg cgcaggaacg
     1981 acgatggaac gcatgcgggc tgctttagaa gcgatcatgc catttaccga gtcttccagc
     2041 gccacgcagg tcagtgggtc aacgcccagt tttgctgcgc agtcgagata tacttgcgga
     2101 tgcggcttgc tgtaaggcag tttttcagcc gaggcgaggg catcgaaact gtcgcgtaag
     2161 tcaaacatgg tcaacacttt ttccagcata tgtagtggtg acgcggaggc cagtcccacc
     2221 aataaacctt gttctttgca taacgcaaca gcttcgcgca cgcctggtaa taatggacgt
     2281 gtctcttcaa ccagtgaaat ggcacgggca ataacccgtt ctactacttc ctgacggctt
     2341 ggcccattcc acggttgccg ggcgtaccaa agatcgacca ccatatcgat gcgtaaacct
     2401 aaggtgtccg gcagctcgtt acgacgggag atatccaccc ccaggcttgc catcacatcc
     2461 agttcggctc gatcccataa aggttctgag tcgataagta atccatccat atcaaaaatt
     2521 gcagcaagaa tctgacgcgg ggttgacatt acaacctctc ctttagagca ttatgtgtaa
     2581 ggtattaagc cgtgcttatg attaagcgcc tatcatatcg cctttaaaga atgggcgaaa
     2641 acagatatct acaggtaaac taagtgaaac gacgcgtctt aatcaaggag aactcatgac
     2701 gtatcaacaa gctggacgca ttgctgtttt gaaaaggatt ttgggttggg tgatttttat
     2761 acctgcattg atctctacat tgatttcttt gctgaaattt atgaataccc ggcaggaaaa
     2821 ccaggaaggc attaatgcgg tcatgctcga ctttactcat gtcatgatcg atatgatgca
     2881 ggcgaatacg ccttttttga atctgttctg gtataactcc ccgaccccta attttaatgg
     2941 tggcgtgaac gttatgttct gggtgatttt catcctgatt tttgtcggac tggcgttgca
     3001 ggattccggt gcccggatga gtcgccaggc gcgcttcttg cgggaaggtg ttgaagatca
     3061 actcattctg gaaaaagcca aaggggaaga ggggctaacg cgtgaacaaa ttgagtcccg
     3121 tattgttgtt ccgcaccata ctattttcct gcagtttttc tcactatata tcctgccggt
     3181 tatttgtatt gctgcaggtt atgtgttctt ttctctactt gggtttattt aatcaaaagg
     3241 ccgcacggag cggccattat cctcaaccca tatcatgctg ctaataatct atccaatgac
     3301 tgctgagcaa taaccaaatg ctgaccgcca aataatcttg cacgatttaa cagcgtgtag
     3361 agttggtaaa ccggttgacg ttcaaggaaa tcggcaggta gcggggatac tgactgatag
     3421 ccgtcataga tttgtggcgg ctgttcagta tgcagcggta acatcgccag gtcgcactct
     3481 cggtcacccc agtagcaggc agggtcgaaa atgtacgggc catccggacc cagcgcacag
     3541 ttgccggacc ataaatcgcc gtgcaacaga gaaggctgcg gttgatgcga ggccagacgc
     3601 tgctgaatat gctcgacgat ggcgtcgata ttgccgaaag cgatcccttt ctctgctgcc
     3661 agttccaact gccagccaat ccgttgttca gcaaaaaacg ttgaccagcg acgttgccag
     3721 gtgttgggct gtggagttgt ggagagcgag ttatcaaaat cgaggccaaa ttgtggttgg
     3781 tcactccatt gatgtaaacg cgcaatttgc tgaccaagaa taaatgcgct atgcgcatcc
     3841 agcggacgag gtgggagata atccatcacc agaaaactgt agtcacggtc agcgcctact
     3901 gcccaaacct taggcacggt gacggtttta ctacgcgaca gtaactccag ttggtcggct
     3961 tcggcggtaa aaccgggaag cagttccctt tcatcacatt tgacgaaaaa gtcatgtcct
     4021 gcatagcgca aatgccatgc ggcatggact tctccgccag gcagttcatt acgcagttcg
     4081 atttcgcctt cacctaactg ctcgctcaaa agacgactga ttgcctgcca catgatgtct
     4141 ctcccatgtt gtctgccaga tcataaggtt agcgcaagaa tgcggtgtaa aaacacgagc
     4201 taacgcacac cacagactga ttattgccca aaaaccattt attttctgtt gctattccat
     4261 tcctcccagg tcgtgatggt gatttcagga tctttgccgg ttgcactttg ggtaagcccc
     4321 gaaaccagtt ctctaatttc ttcttcactt tgggtactga taagtccaaa agtgttagtc
     4381 cccagctcat ggatatttcc ctcatcatcc gtcatggtga gcaaaaaacc gtcgcgagtc
     4441 aggtaattat tcaactcgtt aatttcagtc aatgtatcct catgcaacat gacggttatg
     4501 acataacgga caagatcgcc actggccata gttcacccct ttgttatcac atgacttttg
     4561 agcatagtcg gagaaacgcg ttgccgacag gttggtggtg attcccccaa tgctggggga
     4621 atgtttttgt tagcgggaaa ggtaagcgta aattttttgc gtatcgtcat gggagcacag
     4681 acgtgttccc tgattgagtg tggctgcact ccccgcagct acgccaaaac ggaccatctc
     4741 ttcaagagag gcattttctg ccagtttcag tgtcatcgcg ccgaccatgc tgtcaccagc
     4801 gccaacggta ctctggcttt tcaccggtgg tggcaccacc tgaatgcagt tttcactatc
     4861 aacacccagc gctccttgtg gaccaaggga aacgacaacc cgtttggcct tgccgctatt
     4921 aacgatttcc tgcgcggctt tgcggacatc gtctggctgg gtgagttcgc gattcaccag
     4981 cgcactgagt tctttttggt taggcttaac caactcgatg ttaccaatcg ccagtgctgc
     5041 acttaacgct tcgccagaac tgtcgatgat gcagcggatc ccttggtttt gcgcagcgga
     5101 aatcagttgg gttaattttt ccagcttcac acctggcggc aggcttccgc ttatgaccag
     5161 gatggccccg gattcaattt ccagaacttg ctcttcaagc tggcgaaact catcttcatt
     5221 taatgccgcg cctggcataa caaaacgata ctgctcaccg cttgcttcca catgtacgtg
     5281 taaattctgc cgggtccagt ctttggcttc tacagtagcg acggggacat tttcatccgc
     5341 caacagagaa accaggtgtt cgccggtcgc gccacccgcc gggaagatcg ctgtggcact
     5401 gcctccaaga tgggcaatgg cgcgggcgac gttgatgccg ccgcccccgg gttcgaacac
     5461 cggtgcggta cagcgcagtt ttccttcggg ataaatttgc ggggtaattg ttgcgctatc
     5521 gagagagggc gcaagtgtca acgtatagat acgtaccatc atttcctcct ataggctgat
     5581 ttcagtctgg caccgaatca gccgaaagtg aagaaattaa caatatgatt tattagagct
     5641 ttatttaaaa ttttgcagat aaatatatat aaataaaaat ctgttttcgc cacatgctta
     5701 ttcaaaaaat gaaataagaa tacattgcta agttattcgc gcctttttat aattcgttac
     5761 ctttccaggg acgctaatct ttgatcctta agaaagtttc cgggaccgcg atggtctctt
     5821 tttttgttgt acggactctt gataaatgaa gcttttgaag acagttcctg ccatcgttat
     5881 gttggcgggg ggcatgtttg cgtcactgaa tgcagctgcc gatgattccg tttttactgt
     5941 catggacgat cctgccagcg ctaaaaaacc tttcgaaggt aatctgaacg caggatatct
     6001 tgcacaatca ggcaatacga aaagctcctc acttacggct gacaccacca tgacctggta
     6061 tggacaaacc actgcctggt cgctgtgggg gaacgccagt aatacttctt ctaatgatga
     6121 gcgttcttca gaaaaatatg cggcgggcgg gcgtagccgt tttaatttaa ctgattatga
     6181 ctatttattt ggtcaggcaa gctggctgac agaccgttat aacggctatc gagagcgcga
     6241 tgtgttgacc gcgggttatg gtcgtcagtt tttgaatggc ccggtacaca gtttccgctt
     6301 tgaattcggt ccaggtgtgc gttacgacaa atataccgac aatgccagtg aaactcagcc
     6361 gctgggctat gcctccggtg cttatgcatg gcagttgact gacaacgcta aatttacgca
     6421 gggtgtttcc gtctttggcg cggaagacac aacgctgaac tccgaaagcg cattaaatgt
     6481 tgctattaat gaacattttg gactgaaagt ggcttacaac gtcacatgga actctgaacc
     6541 tccagagtct gcaccggaac ataccgatcg tcggacaacc ttatcgctgg gctacagcat
     6601 gtaaaattat ctgccggacc gtgttatgcg gtctggctgt cttcgtttgt cactgctcag
     6661 ccccaaatca ttgctgccca acgtagcaac aacatagcgc cacagattgc cagtagcacc
     6721 accaccatct tccagtgttt tttatcaaag cgtttagtcg gcataccagt gtcctgcata
     6781 tctcttgtgc gtgataacgg agtgtaacaa aagggacagg catcaaggga ttttttgtta
     6841 ttacagccgt cgggtggcgt tgtacacctg atcactgttt ctgcgtttta tgaaaaaaat
     6901 aaaattatgt atcgatatac aaatatcata aatagctggt tataaatacg tttttagttc
     6961 atcagcacta aaactcaggc tttggctgat tgcattaata gcagcataat atgtttctac
     7021 agttttataa tgattatgat gaatggctat atgaacagct gacatgtttt catgattttt
     7081 agcggccaac agtgtgaata attgacgttg actaaaagaa tatttttttg caaaagtact
     7141 cagtgttgat atataagata acacgacgtc tttattaccc ttgctcatgg cgatgtataa
     7201 agcaggggtt ccatgtgctg tcgcgccttt taataagtcc atgatgttga ttttgctgag
     7261 tttatattta acggcgatcc cataaacttt agagaggaac cgtgtgaaac acaaagggtg
     7321 attattttcc attgcagcaa ataaccctgg ttcaccatca cgtgttttca tggctaaaat
     7381 atccatcacc atttttttgt ttaaaagttc ttgtgctatt ggagtggtta atgcattcag
     7441 aaagatatcg attatttcat cgaatccata caacatggag atatagagtc caggtgtttc
     7501 gttagcactt ttagtttgaa gtaatttaac cagattatct tcatgaatga tatgattgta
     7561 taccagtgac tggatttctt gcataaatat ttttacgttg tcagtttgtc cgttcatcat
     7621 agccagaaat aaagcaggtg taccaacgct attctgcgca gttattattt caatgatttc
     7681 tttttcaggg agtatgggca actgtttgaa tagtgtaata agctattgag tctggcctgt
     7741 ggccattatt aacaacattg ccgatggatg tataggggca aatggcattc gtgtcgtgat
     7801 acccgctaaa atagcaactg gaactccctc aggtaataga ggaagaatag caattgcaca
     7861 gttgcgaaca tacctatcag cccaggtctg agcatcggga gctgaactga tgaacttatc
     7921 aagcgagaga aagcgccgtg ctgtcctgtg ggtgcctctg acggcaacgt tggtatcgtt
     7981 cgggtcgtaa aaagacacaa cataatttaa ttgtccatac actgactgct ttattcgtag
     8041 ccttactgac aaagtatgat tgtctatata taaaatcgcg gacatatgac tgacattgtt
     8101 tgccatcatt cgacaaaaga aaatatctaa agcgaagcca aaatccttac tcgcgataac
     8161 gcgactccct gcaggaggat ggcgaagtaa tgctctgaac tccgcatccc ggactaaggg
     8221 gatatgctgc tgtatggatt caacggagga gtaatgttta atcaggttga ctttgccttt
     8281 tccatttctc aatgaatcaa gggcgtattg caatgacaga tggcgacaaa aaatagcgtc
     8341 agaaggagat tgcaaaaaac atgcactacc attgagattt actgttgggt gtatagtttt
     8401 ttctctatgg atttttttag acagtttcat acaatggtta cgatcttcag cagttaactg
     8461 ttcctgttca atataatcca gtatgcttcg caaatggtta gctttaatag gcgtggtttc
     8521 cagtaataag gaaatgaaat cttttttata gtgtaatgta atgagtatat tttctgcatg
     8581 gatgggtgaa gtggcaaagt taatggtaaa aagggcagtg gaataacaat tttgtgatat
     8641 ttttcgacag tttgcgataa attcctgaat aacacagtat gacagcatcg aacttaagtc
     8701 agaggtatca agtagggtga tgagctggcg cgcaaattta ttaataggct ctgatgaagg
     8761 tgttggtatg tcgctatcga tgtgcaatct atgaggagac ttataatgag ttctgataat
     8821 gatatcgttt tgactcataa tctattcgcc ttatatatat ctggaggttt tgaggatgat
     8881 gattaattat tatcgccaga acgaaatgtg cacgaaaaat tgttataaga attgtgtcac
     8941 ttttatcatt gtgatatgac cacttcgtca ttaatctcat gagcgtttaa aatactaccg
     9001 ccaaatcacg caacgaactt tgctggattc agtttatgct gctgtaaatc cgctcgcagt
     9061 aaacctttca gacgcaccgt gatgttatca gtggttcttt aagcgttttg ctggtgtact
     9121 cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt
     9181 ttagtgttaa catcgctcaa ccgggtggaa ggtgagaggt taatgtcttt catttgtgta
     9241 cctgattgcg aaccaattta gcatttgttg gctagatggt ttcgcaatga actgttaata
     9301 aacaaatttt tctttgtatg tgatctttcg tgtgggtcac cactgcaaat aaggatataa
     9361 aatgcctgtt ataactcttc ctgatggcag ccaacgccat tacgaccacg ctgtaagccc
     9421 catggatgtt gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt
     9481 taatggcgaa ctggttgatg cttgcgatct gattgaaaac gacgcacaac tgtccatcat
     9541 taccgccaaa gacgaagacg gtctggagat cattcgtcac tcctgtgcgc acctgttagg
     9601 gcacgcgatt aaacaacttt ggccgcatac caaaatggca atcggcccgg ttattgacaa
     9661 cggtttttat tacgacgttg atcttgaccg cacgttaacc caggaagatg tcgaagcact
     9721 cgagaagcgg atgcatgagc ttgctgagaa aaactacgac gtcattaaga agaaagtcag
     9781 ctggcacgaa gcgcgtgaaa cattcgccaa ccgtggggag agctacaaag tctccattct
     9841 tgacgaaaac atcgcccatg atgacaagcc aggtctgtac ttccatgaag aatatgtcga
     9901 tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc catcatttca aactaatgaa
     9961 aacggcaggg gcttactggc gtggcgacag caacaacaaa atgttgcaac gtatttacgg
    10021 tacggcgtgg gcagacaaaa aagcgcttaa cgcttacctg cagcgcctgg aagaagccgc
    10081 gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg taccatatgc aggaagaagc
    10141 gccgggtatg gtattctggc acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt
    10201 tgttcgttct aaactgaaag agtaccagta tcaggaagtt aaaggtccgt tcatgatgga
    10261 ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa tgttcaccac
    10321 gtcttctgag aaccgtgaat actgcattaa gccgatgaac tgcccgggtc acgtacaaat
    10381 tttcaaccag gggctgaagt cttatcgcga tctgccgctg cgtatggccg agtttggtag
    10441 ctgccaccgt aatgagccgt caggttcgct gcatggcctg atgcgcgtgc gtggatttac
    10501 ccaggatgac gcgcatatct tctgtactga agaacaaatt cgcgatgaag ttaacggatg
    10561 tatccgttta gtctatgata tgtacagcac ttttggcttc gagaagatcg tcgtcaaact
    10621 ctccactcgt cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga
    10681 cctggcggtt gcgctggaag aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc
    10741 tttctacggt ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg catggcagtg
    10801 cggtacagta cagctggact tctctttgcc ttctcgtctg agcgcttctt atgtaggcga
    10861 agacaatgaa cgtaaagtac cggtaatgat tcaccgcgca attttggggt cgatggaacg
    10921 tttcatcggt atcctgaccg aagaattcgc tggtttcttc ccgacctggc ttgcgccagt
    10981 tcaggttgtt atcatgaata ttaccgattc acagtctgaa tacgttaacg aattgacgca
    11041 aaaactatca aatgcgggca ttcgtgttaa agcagacttg agaaatgaga agattggctt
    11101 taaaatccgc gagcacactt tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga
    11161 ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga
    11221 cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc agccgcagtc ttaaacaatt
    11281 ggaggaataa ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa
    11341 tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc agcttggtat
    11401 tgtgagtctg agagaagctc tggagaaagc agaagaagcc ggagtagact tagtcgagat
    11461 cagccctaac gccgagccgc cggtttgtcg tataatggat tacggcaaat tcctctatga
    11521 aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat
    11581 taaattccgt cctggtacag atgaaggcga ctatcaggta aaactccgca gcctgattcg
    11641 ctttctcgaa gagggtgata aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc
    11701 gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc
    11761 agtggtcgaa tccttcccaa cgaagatcga aggccgccag atgatcatgg tgctcgctcc
    11821 taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg gagccttcgg gcttcacagg
    11881 ctttgttcgc ctttgtttcg tttattaaca atgcgaagtg gaagttatta aaatgccaaa
    11941 aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa
    12001 gcacaagcac gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca
    12061 cctgcgtccg aaagccatgg tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc
    12121 gtacgcataa gccgttaacg tttttaactt tttaattaga atatagatac aggagagcac
    12181 atatggctcg cgtaaaacgt ggtgttattg cacgcgcacg tcacaagaaa attttgaaac
    12241 aagctaaagg ctactacggt gcgcgttctc gcgtataccg cgttgccttc caggctgtta
    12301 tcaaagctgg tcagtatgct taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac
    12361 tgtggattgc gcgtatcaac gcagcagcac gtcagaacgg tatttcttac agcaaattca
    12421 tcaatggcct gaaaaaagcc tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag
    12481 tattcgacaa agtagcgttc accgctctgg ttgaaaaagc gaaagcagct ctggcataag
    12541 ccagttgaaa gagggagcta gtctccctct tttcgtttca acgccatcaa aacattgact
    12601 tttatcgccg tagccttttc aataaaggtc ttttgaagag taccaaaagg taacgcaagc
    12661 aatgaatgct gctattttcc gcttcttttt ttactttagc acctgaatcc aggaggctag
    12721 cgcgtgagaa gagaaacgga aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt
    12781 atgcgcgttt tgaaaaataa aaagcctgtc tcatcaggtg taattgtcgc agccagtctg
    12841 gacacggcca gcgcggagaa accggagcgt atttaagtac gtgagaattt cgagcactgc
    12901 ccgggaccaa aatggcaagt aaaatagcct gatgggatag gctctaagtc caacgaacca
    12961 gtgtcaccac tgacacaatg aggaaaacca tgtcacatct cgcagaactg gttgccagtg
    13021 cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat
    13081 atttgggtaa aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag
    13141 aagagcgtcc ggcagctggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc
    13201 tgaatgcgcg taaagcggaa ctggaaagcg cagcgctgaa tgcgcgtctg gcggcggaaa
    13261 cgattgatgt ctccctgcca ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc
    13321 gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc
    13381 cggaaatcga agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg
    13441 cgcgcgctga ccacgacact ttctggtttg acactacccg cctgctgcgt acccagacct
    13501 ctggcgtaca gatccgcacc atgaaagccc agcagccacc gattcgtatc atcgcgcctg
    13561 gccgcgttta tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag
    13621 gtctgattgt tgataccaac atcagcttta ccaacctgaa aggcactctg cacgatttcc
    13681 tgcgtaactt ctttgaggaa gatttgcaga ttcgcttccg tccttcctac ttcccgttca
    13741 ccgagccttc tgcagaagtt gacgtcatgg gtaaaaacgg taaatggctg gaagtgctgg
    13801 gttgcgggat ggtgcatccg aacgtgctgc gtaacgttgg catcgacccg gaagtttact
    13861 ctggtttcgc cttcgggatg gggatggagc gtctgactat gttgcgttac ggcgtcaccg
    13921 acctgcgttc attcttcgaa aatgatctgc gtttcctcaa acagtttaaa taaggcagga
    13981 atagattatg aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag
    14041 cgatgcgctg gcgaatcaaa tcactatggc gggcctggaa gttgacggtg tagaaccggt
    14101 tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa
    14161 cgctgacaaa ctgcgtgtga caaaagtgaa tgttggcggc gatcgcctgc tggacatcgt
    14221 ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg gtagctacca ttggtgctgt
    14281 tctgccgggt gatttcaaaa ttaaagcggc gaaactgcgt ggtgaaccgt ctgaagggat
    14341 gctgtgctcc ttttctgagc tgggaatttc tgacgatcat agcggcatta tcgagctgcc
    14401 tgcggatgcg ccgattggca ccgacatccg tgaatacctg aaacttgatg acaacaccat
    14461 cgaaatcagc gtgacgccaa accgtgccga ctgtttaggt atcattggtg ttgcgcgtga
    14521 cgttgccgtg ctgaatcagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac
    14581 catcgacgac acgctgccga ttacagtcga agcgccggaa gcctgcccgc gttatcttgg
    14641 ccgtgtggta aaaggcatta acgttaaagc gccaactccg ctgtggatga aagaaaaact
    14701 gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtactgct
    14761 cgaactgggc cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt
    14821 gcggatggcg aaagagggcg aaacgttggt actgcttgat ggcactgaag cgaagctgaa
    14881 tgctgacact ctggtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg
    14941 tggcgaacac tctggcgtga atgacgaaac gcaaaacgtc ttgctggaat gcgctttctt
    15001 cagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca
    15061 ccgttatgag cgtggcgttg atccggcact gcagcacaaa gcgatggaac gtgcgacccg
    15121 tctgctgatc gacatctgcg gtggtgaggc tggtccggta attgatatca ccaacgaagc
    15181 aacgctgccg aagcgtgcaa ccatcacttt acgtcgtagc aaactggatc gcctgatcgg
    15241 ccatcatatt gcggatgagc aggtaactga cattctgcgt cgtctcggct gcgaagtgac
    15301 cgaaggcaaa gacgagtggc aggcagttgc gccgagctgg cgtttcgata tggagattga
    15361 agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc
    15421 ggtacaggca agcctgatta tgggtactca ccgtgaagct gacctgtcgc tcaagcgcgt
    15481 gaaaacgctg ctcaacgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc
    15541 gaaagtgcag cagatgatcc atccaggcgt tgaagcctta ctgctgccaa gcccgatctc
    15601 tgttgaaatg tcagcaatgc gtctttctct gtggaccggc ctgctggcaa ccgtggtgta
    15661 caaccagaac cgtcagcaga accgtgtgcg cattttcgaa agcggtctgc gtttcgtacc
    15721 agatactcag gcaccgttgg gcattcgtca ggatctgatg ttagccggtg tgatttgcgg
    15781 taaccgttac gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa
    15841 aggcgatctt gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc
    15901 agaagcgaat ccggcactgc atccggggca atccgcagcg atttatctga aaggtgaacg
    15961 tattggtttt gttggggttg ttcatcctga actggaacgt aaactggatc ttaacggtcg
    16021 cactctggtg ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg
    16081 cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt
    16141 tcccgcagcg gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt
    16201 aaacttattt gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat
    16261 aagcctgatc ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt
    16321 cgccaaatgt gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta
    16381 tggcgcttac aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc
    16441 gggatgccaa agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg
    16501 gcgaacaggt gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc
    16561 cgggacgtaa cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct
    16621 tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat
    16681 ctgatctaac taaaaaggcc gctctgcggc cttttttctt ttcactgtcg aagagtcacc
    16741 gtaaaatcaa cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa
    16801 caacagcgac aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt
    16861 ctcttaagcc tttgcgccgg tgaacaatgg atctcgccag gtgactggtt ttctcctcgt
    16921 ggcgaactgt tcgtctggca gattcgcctg ccacgtacgc tggctgtatt gctggttggt
    16981 gcggcgctgg ctatatccgg cgcagtaatg caggcgttgt ttgaaaatcc tctggcagaa
    17041 cctggactac ttggcgtctc taacggcgca ggcgtagggc ttatcgccgc ggtattgctt
    17101 gggcaagggc aactcccaaa ctgggcacta gggctgtgtg cgattgctgg cgcgcttatc
    17161 atcactttaa tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg
    17221 gctggcgttg cattagggat tatctgtagc gcactaatga cgtgggctat ctacttttcc
    17281 acctctgttg atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac
    17341 tggcggcaaa gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag
    17401 tccaggccga tgaatatgtt agcacttggc gagatctcgg cgcggcaact gggtttaccc
    17461 ctgtggttct ggcgcaatgt gctggtggca gcgaccggct ggatggttgg cgtcagtgtg
    17521 gcgctggcgg gtgctatcgg ctttattggt ctggtgatcc cacatattct tcggttgtgt
    17581 ggtttaaccg atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg
    17641 ctgctggccg atgttgtagc gcgcttggca ttagctgccg cagagctgcc tattggcgtg
    17701 gtcaccgcaa cgttgggtgc gccggtgttt atctggttat tgttaaaagc aggacgttag
    17761 ccgcaaaaag acggtctatg attaaaagct aagattttta ctgaccacac ccaggagaaa
    17821 cgatgcaaga ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc
    17881 tggagaagta cgccggtaat gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa
    17941 cgccgcaata tgagcagttg gagaatattc agaaagcctg ggccgatcga ggttttgtgg
    18001 tgctgggatt cccgtgcaac cagtttctgg aacaagaacc gggcagcgat gaagagatta
    18061 aaacttactg taccaccaca tggggggtga cgttcccgat gttcagtaag attgaagtta
    18121 atggcgaagg acgccatccg ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg
    18181 cgccggaaga gagcggattc tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc
    18241 ctgatgatat tttatggaat tttgagaaat ttttagttgg cagggacgga ttggtcatcc
    18301 agcgtttttc cccggatatg acgccggaag accccattgt gatggaaagc attaaactgg
    18361 cgttggcaaa ataatgtcta ttgtgatgca gttacaggat gttgcggaat ctacccgcct
    18421 ggggccactt tttggcgagg ttcgggctgg ggagatcctg catctggtgg ggccgaatgg
    18481 cgcgggtaag agtaccttgc ttgcacgaat ggccggaatg accagcggta agggaagcat
    18541 tcagttcgcg gggcaacccc tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc
    18601 ctatctttca caacagcaga cgccgccgtt tgcaatgccg gtctggcact acctgacact
    18661 gcatcagcac gataaaacgc gtaccgaact actgaatgat gtcgcagggg cgctggctct
    18721 tgatgacaaa ctcggacgta gcaccaatca actttccggc ggtgaatggc aacgcgtacg
    18781 tcttgctgcg gtggtgctgc aaatcacacc acaagccaat cccgcaggtc aattgctgct
    18841 tcttgatgag ccgatgaaca gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct
    18901 gagcgctctg tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccacac
    18961 attgcgtcat gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg
    19021 cagagaagag gtgctcacgc cgccaaatct ggcgcaggcc tatgggatga attttcgccg
    19081 tctggatatc gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcttga
    19141 aaatcgggta attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga
    19201 atgcgtttct gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccaccataaa
    19261 gcaccgccgc caaatgccag actttctgat tcgattaccg ttattgccgg tttgaacgac
    19321 cagctacaaa gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg
    19381 gactgttcgg gatttgtggt tgtaacgatg cgcgatcgtt tcgatttgca gctgccccga
    19441 gaaaccaaag aacaagcctc tatcggcacg caaattgata aagacgagtt gctgcctggt
    19501 gacctggtct ttttcaaaac ggggtccgga caaaatggtt tgcatgtagg tatttacgat
    19561 accaacaacc aatttatcca cgcctctacc agcaagggag tgatgcgttc ctcacttgat
    19621 aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa tctagtatgt cgaaaaatgg
    19681 acggcgatga tacatcgccg tccatttact cctgaataca gtagtatttt gcagacaaaa
    19741 attccgtaaa tggattattc gtttataatc aggataatct aaaaaaagga cccctgaaaa
    19801 agtggttaac atggaacaaa tgaaagttac tccttctgta attgttatgt cttatggcct
    19861 catccctttt agccggatac tgaaaaacat ccttcgagag ggacggttac catgaagatt
    19921 tttttggaga atctttatca ttctgattgc tatttcctgc cgataagaga taaccagcaa
    19981 ctccttgtcg gcgttgagtt aattacccat ttctccagtg aagatggcac ggtgcgtatc
    20041 ccgacgagtc gggttatagc gcaacttacc gaggagcaac attggcaatt attttcagag
    20101 cagttggaat tactaaaatc atgccaacat ttttttattc aacataaact ctttgcctgg
    20161 cttaatttaa cgccacaagt ggctacatta ttattagaga gggataatta cgcaggcgaa
    20221 ctattaaaat atccgtttat tgaactgttg attaatgaga attacccgca tctcaatgaa
    20281 ggaaaagata atcgcgatct tttatcctta tcgcaaatgt atccccttgt gttaggcaat
    20341 ttgggagcag gtaatagcac aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg
    20401 ctggataaaa gttttattca gcaacaaatc acgcatcgct ccttcgaacc gtttattcgt
    20461 gcgatccagg cgcaaatttc cccttgttgc aattgcatca tcgcgggggg cattgatacg
    20521 acagaaatat tggcacagat tacccccttc gatttccatg ctctccaggg atgcctgtgg
    20581 cctgccgttc ctataaatca gataacgacg ttggttcagc gataaccctc ctgtttgctg
    20641 gtgtttaaga cgagagtaac cgtctacact atcaaacagg aggatctatg accctgtctt
    20701 ttattacccg ctggcgcgat gaattgccag aaacctatac agcactttcc cctacgcctt
    20761 taaataatgc ccggctgatt tggcataata ccgaactcgc taacacgctg agtattccat
    20821 cgtcgctgtt taaaaatgct gcaggtgtct ggggcggcga aaccctactg cctggtatgt
    20881 caccactggc gcaggtttac agtggtcatc agttcggcgt ctgggcgggc caactgggtg
    20941 atgggcgcgg cattttactt ggcgaacaac tgcttgctga tggcactaca atggactggc
    21001 atctgaaagg tgctggcctg acgccttatt cgcgaatggg tgatggacgg gcggttttac
    21061 gttcgacgat acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga
    21121 cccgcgcgtt aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtggagccag
    21181 gcgcgatgct gatacgtgtg gcaccaagtc atctgcgctt tggtcatttc gaacattttt
    21241 actatcgccg cgagccggaa aaggttcgtc agttagctga ctttgccatt cgtcattact
    21301 ggtcacatct tgcagatgat gaggacaaat accgtctctg gtttaccgat gttgtcgcac
    21361 gtaccgcatc gttaattgcc caatggcaga cggtcggctt tgctcatggg gtgatgaata
    21421 ccgacaacat gtcgctgctg gggctgacgc ttgattacgg gccgtttggt tttcttgatg
    21481 attacgaacc cggttttatt tgtaatcact cggatcatca agggcgttat agctttgata
    21541 atcaacctgc tgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg
    21601 ttgccgtaga tgccctgaat gaggccctgg acagctatca gcaggttttg ttgacgcatt
    21661 atggacaacg gatgcggcag aaactgggct tcatgacgga gcaaaaagag gataacgcgc
    21721 tactgaatga attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacattcc
    21781 gcatgctgag tctgaccgag cagcacagcg cggcgtcacc gctacgtgat gagtttattg
    21841 atcgtgcggc atttgatgac tggtttgccc gttatcggag gcgtttgcaa caagacgagg
    21901 ttagcgatat tgagcgtcag caactgatgc aaagcgttaa cccagctctg gtgttgcgca
    21961 actggttggc gcaacgggcg attgaagcgg cagaaaaggg tgatatgacg gaattgcacc
    22021 gcctgcatga ggcgttgcga aatcctttca gcgacagaga tgatgactat gtcagtcgtc
    22081 cacctgactg gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttagttaac
    22141 agcagcttgc cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc
    22201 agtttgccat ccggacctaa cagtgtctgg ctggaaattc gccgaatagg ctgcggggaa
    22261 gcggtcttgt gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata
    22321 aaaattaata ataattgata taacaatgat tcttattatc aatattatgg ctaaccagag
    22381 caagcagtaa tgtgctgaga agacaacaag ttgcgcagaa atgcccatcc catcaggaat
    22441 gggcacgcat tcagaagcgg gtatctaccg cagaggcgag tttttcgacc aggcgttcgg
    22501 tatcttccca gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgactgc
    22561 cgacgatttt ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatcgccg
    22621 tagagccatt gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct
    22681 gcttctggca gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt
    22741 gcagcgtatc gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac
    22801 gcataataat gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt
    22861 tgtctggcga gaggaacata tggctggcgc gggctgcgcg gatagcatcc acggcaatcc
    22921 gcgtgttgcc atcggtacca tttttaaaac ctaccggaca ggagagtgcc gaagccattt
    22981 cacggtggat ctgactttcg gtagtacgtg cgccaatcgc gccccaactg attaaatcag
    23041 caataaactg accggtcacc atatcgagga actcagtcgc ggtagggaca cccagctcat
    23101 ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctgccgt
    23161 ttaaatccgg atcggagatt agccctttcc agccgacaac agttcgtggt ttttcaaaat
    23221 aggtgcgcat tacgatttcc agccgtgact ggtactggtt gcgcagcgac tgcagacggg
    23281 tggcgtactc cattgcagcg gtgagatcgt ggatcgagca ggggccaata ataaccaaca
    23341 gtcgcttatc ttcaccattc agtatttttt caattctgcg gcgggagtcg gtgacatggg
    23401 cggcgacgcc aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct
    23461 caatacgcgc agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc
    23521 ttctacggca ggactgccgg gaagtgatgc gcatcacgat aaaccaatcg ctgctgattt
    23581 caagtgcgag gtgtgtcaca cctcgccggg tggttagcat gataacaaaa aaataagcta
    23641 atgcactagt tctctagtac attcggcgac taaggcccat gatatcgagg atcttggtgg
    23701 caatctcttc taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca
    23761 acgcttccac ttccgcgact tccatcctgc actgacgaag cgaggcatag cgactgttct
    23821 cccgacgttc ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt
    23881 tgtgctgaag cggtttgagc gacgcgggta gcaccagatt atccatatcg tcggcaataa
    23941 aggggtagtt tgccgcgcgg ataccaaatt gcattgccag atacagactg gtgggggttt
    24001 taccacagcg agaaacgccg agcaggatca cctgagcctg gtccagattg cgcaacgaaa
    24061 taccgtcatc gtgggcaagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga
    24121 gattattagg gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct
    24181 cttgttgtag cggggcaacc agcgcctgaa cgatatcctg gcaaaagcct tcactttgca
    24241 agatgatggc gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacacctg
    24301 tctggtgata aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga
    24361 caaacggcag cgtaatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta
    24421 atacctccgc agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat
    24481 tatccatttt gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg
    24541 cacagaagcg taggacgtaa tgtctggttt ataaaatgaa ccttcaattt tattttttat
    24601 gaaaacagca tttcattttt atggtttcgt ttataccgat ggtttatgtg gaaattgtcg
    24661 aatagagcag gtttgcgcaa cgctgggatc agtcttaaaa agtaaaaaaa tatatttgct
    24721 tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc gcagaaatgt
    24781 gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa tggctcgtca
    24841 ccgctggtgc tttggtataa ccaactcggc atgaatgatg tagacagggt tgggggcaaa
    24901 aatgcctccc tgggtgaaat gattactaac ctttccggaa tgggtgtttc cgttccgaat
    24961 ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg cgtaaaccag
    25021 cgcatttatg aactgctgga taaaacggat attgacgatg ttacccagct tgcgaaagcg
    25081 ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct ggaaaatgcc
    25141 atccgcgaag cttatgcaca gctttccgcc gatgacgaaa acgcctcttt tgcggtgcgc
    25201 tcctccgcta ctgcagaaga tatgccggac gcttcctttg ccggtcagca ggaaaccttc
    25261 ctcaacgttc agggttttga cgccgttctc gtggcagtga aacatgtatt tgcttctctg
    25321 tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg tggcgtggcg
    25381 ctctccgccg gtgttcaacg gatggtgcgc tctgacctcg catcttctgg cgtgatgttc
    25441 tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc atggggcctt
    25501 ggtgaaatgg tcgtgcaggg cgcggttaac ccggatgagt tttacgtcca taaaccgaca
    25561 cttgcagcga atcgcccggc tatcgtgcgt cgcaccatgg ggtcgaaaaa aatccgcatg
    25621 gtttacgcgc cgacccagga gcacggcaag caggttaaaa tcgaagacgt accgcaggaa
    25681 cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa acaggccgta
    25741 caaattgaga aacactacgg tcgcccgatg gatattgagt gggcgaaaga tggccacacc
    25801 ggcaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg tcaggtcatg
    25861 gagcgttata cgctgcattc acagggtaag attatcgccg aaggccgtgc tatcggtcat
    25921 cgcatcggcg cgggtccggt gaaagtcatc catgacatca gcgaaatgaa ccgcatcgaa
    25981 cctggcgacg tgctggtcac tgacatgacc gacccggact gggaaccgat catgaagaaa
    26041 gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat catcgctcgt
    26101 gaactgggca ttccggcggt agtgggctgt ggtgatgcaa cagaacggat gaaagacggt
    26161 gagaacgtca ctgtttcttg tgccgaaggt gataccggtt acgtctatgc ggagttgctg
    26221 gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg atctgccgtt gaaagtgatg
    26281 atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct gcctgccgaa cgaaggcgtg
    26341 ggccttgcgc gtctggaatt tatcatcaac cgtatgattg gcgtccaccc acgcgcactg
    26401 cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat gatgaaaggt
    26461 tttgattctc cgcgtgaatt ttacgttggt cgtctgactg aagggatcgc gacgctgggt
    26521 gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc gaacgaatat
    26581 gccaacctgg tcggtggtga gcgttacgag ccagatgaag agaacccgat gctcggcttc
    26641 cgtggcgcgg gccgctatgt ttccgacagc ttccgcgact gcttcgcgct ggagtgtgaa
    26701 gcagtgaaac gagtgcgcaa cgacatgggg ctgaccaacg ttgagatcat gatcccgttc
    26761 gtgcgtaccg tagatcaggc gaaagcggtg gttgaagaac tggcgcgtca ggggctgaaa
    26821 cgtggcgaga acgggctgaa aatcatcatg atgtgtgaaa ttccgtccaa cgccttgctg
    26881 gcagagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga tatgacacag
    26941 ctggcgctcg gtctggatcg tgactccggc gtggtgtctg aattgttcga tgagcgcaac
    27001 gatgcggtga aagcattgct gtcgatggct atccgtgccg cgaagaaaca gggcaaatat
    27061 gtcgggattt gcggtcaggg tccgtccgac catgaagact ttgccgcatg gttgatggaa
    27121 gaggggatcg atagcctgtc tctgaacccg gacaccgtgg tgcaaacctg gttaagcctg
    27181 gctgaactga agaaataaaa taaatccccg gcggcgttca gtcgccgggt ttatgtgatc
    27241 cccgaagata aaacttattc aatctcttca cagacatcct gcgttaatcg ccgcataata
    27301 tcttttctta acaaaaactt ttgtatttta cctgaggcag tgcgcggtag tttttcgatt
    27361 accacgatat gttcaggata tttatatttt gcgacccgtt tacggctaaa aaaagccacc
    27421 acctcttcca gcgataatga atgatgcggc gctttcagca cgacataagc gcatgatcgt
    27481 tcacctaaac gttcatcggg cattgcaacc acacaggcat cgtgaatttt aggatgctgc
    27541 aataaaatat cttccacttc acggctgcta atattttcgc cgccgcggac aataatatct
    27601 tttttgcgtc cggtaatttt tatatagcca gcctcatcca tacggcagag atcgccgctg
    27661 taataccagc cttcttcatc cagcgcacgg gcggttaatt caggttcatc aaaatatccc
    27721 ataaacacat tggggccacg cgaggcttct tcaccttcgc aacctggcgg taaggtcttg
    27781 cgtgcgtcat cgaccacttt aatctctaca cctgcggcag cgtaaccatc ggtgtgcata
    27841 aagcgcgaca aaggatcatc gagattcacc accgcatgcg gcgaactttc tgtggaacca
    27901 taaacactta ataatttaat gccgagctgc tggcattcac gcgccacttt tttggggatt
    27961 gtggttccgc cgcaaagaaa gaaacgcagc gctgacaggt ccgcgggttg tttctctagt
    28021 acattcaaaa gatcatagac aaacggcgtt gcgcctagca tacaggtgca acgctgctgc
    28081 tcaagcagcg cgagacacgc atcaggagtg aaaatatcta acaacacgct gcgagcgcca
    28141 attaagaatg gtgccgttac gccatgcaga aagcccgttg cgtggccgag tggcgcaggc
    28201 atcataaaga catcctgcca ggtcagattc agccgcgcgc aataagcccg ctcactggcg
    28261 agaatattgt tatgcgttag catcacgccc tttggcagac cctcggttcc ggaggtaaac
    28321 agcaccgcag ctaattcatc gccgtgggtc gttatcgccg tggtcagtgg ggtattgtcg
    28381 gcgataatct gacttaatga gagggaagag gtggcgggag ccagtttgtc cacgccgaca
    28441 atttgttgta gttgtggaag ctgattttgc agcggcagga ttaaatctac cgggcgcgtt
    28501 tgtttaaaca acgtcggtgc aaagaacatt tttgcctgac acttattgag cacccacacc
    28561 agttctgctt cccgccagga aggcaacagc ggcacggaaa ccgcaccgat tttcaggcag
    28621 gcaagataga taacggtaaa ttcacaccag ccaggcagtt gaaatgcgat gcgatcgcct
    28681 gattcaatac ccttcgctaa catccagttt gccagacagc tcgcggcgtg atcgagcgcg
    28741 ctataggtgt acgatgcacc atgattatcg accacggcaa ttttgtctgg catcgcacga
    28801 gcggtctgct gccagtaatc ggccagcgaa gcatcgcccc atagcccttg ctgacgatac
    28861 gccgcacgac gttgttcgtt gaacgttaat gtcactttca tgttggactc tcgaaacaga
    28921 acatcaggcc cgagatgcgg gcctgtggga tgcatgatta gccgtagcga aaatcaatac
    28981 cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt gttaccgcac agcacccgac
    29041 aggtgccgca ctccagacaa ccggcagaat caaaatgaat gttgccggcg tcatcctgct
    29101 tataaagtcc ggcagggcag gctttcatta atttacggaa ttcattgata tcgggatttg
    29161 ccgccagaat gatatgcggg tggccctcat caacatggaa tttattgacg cctaatttga
    29221 tgtcgacgtt aaccgtagcg ttctggctca tagcgcggtt gctcccttaa tgccatcttt
    29281 cagcaagttg atcagcccaa ttttcttcgc atgtcccatg atcattttgc gtaccggctg
    29341 gtttggtttg ccgtcaatgg tgaacatctc gttcatgatg tcggcgacca ttcgtgggta
    29401 ttggctaaac aggcgcgggt tttccatcag cgccgggatc ttgcggaaat gctgcatatc
    29461 gcgcatgacg cagctttgtt ccagctcacg tttgtattgc gccagactgc tggcggagaa
    29521 atcctcgcgt tctttggcgg cgatcaccgt tgtggcggca gcctgagccg atgcaatggc
    29581 taaatccatg ccacgaactg taaaacccaa attcaggcag aagcctgcgg cgtcaccaac
    29641 gatcatcacg ccatcgttaa ccatctgcgg caccattgcc agaccgcctt ctggcaccat
    29701 atgcgcggaa tattcaagca gtttgccgcc gctaattagt gggcgaatgg cggggtgttg
    29761 tttaaaatct tccagcattt gcggcacgct tttttgcgta tgggcgatat cacccaatcc
    29821 acaaaccagc cccaaggata tggaatcctt gttggtatag agaaatcccc cgcccatcag
    29881 gccgtcagaa ggggaaccgg caaacagcca ggcggcacct tcctcgcccg taatattaaa
    29941 gcgatcgttg atctgttctg gtgtgaggcc aataacctct ttaacaccaa cagcgtaatg
    30001 atgcggatcg gaagcgggaa ccattcccag cgagcggcca agcatcgagt taacgccatc
    30061 agccagaatc accacattcg cttcgagaat atcatcccca gcctgcacgc cagtgacctt
    30121 gtttccttca cgaaccagcg catcgacgcg aacgcctggg ataaactgcg cgcccgcctg
    30181 ctcggcttgt tccatcaacc acgggtccag acgattacgc aatacggtat aagatgcgtg
    30241 ttgcggaaca tctggttgct cgcggtgaaa atcgagggta acggcgcttt cttcggttaa
    30301 gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc gctgatactg caaagcctgg
    30361 aatgattgct tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt tacatccggc
    30421 actgtcgccg cgttctatca ccaggacatc cagcccggct cgcgccatga ccagtgcggc
    30481 aacgctacca gcaacgcccg caccgac
//
LOCUS       DAFATA010000048        30253 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFATA010000048 DAFATA010000000
VERSION     DAFATA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.049"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..100)
                     /locus_tag="LGJ20_002613"
     CDS             complement(<1..100)
                     /locus_tag="LGJ20_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2386656.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFN"
     gene            108..1070
                     /gene="tnpA"
                     /locus_tag="LGJ20_002614"
                     /pseudo
     CDS             108..1070
                     /gene="tnpA"
                     /locus_tag="LGJ20_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1279..1917)
                     /gene="leuE"
                     /locus_tag="LGJ20_002615"
     CDS             complement(1279..1917)
                     /gene="leuE"
                     /locus_tag="LGJ20_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBK2386657.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2044..2967)
                     /gene="dmlR"
                     /locus_tag="LGJ20_002616"
     CDS             complement(2044..2967)
                     /gene="dmlR"
                     /locus_tag="LGJ20_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBK2386658.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3070..4155
                     /gene="dmlA"
                     /locus_tag="LGJ20_002617"
     CDS             3070..4155
                     /gene="dmlA"
                     /locus_tag="LGJ20_002617"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBK2386659.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4406..6016
                     /gene="yeaV"
                     /locus_tag="LGJ20_002618"
     CDS             4406..6016
                     /gene="yeaV"
                     /locus_tag="LGJ20_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBK2386660.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6048..7172
                     /gene="yeaW"
                     /locus_tag="LGJ20_002619"
     CDS             6048..7172
                     /gene="yeaW"
                     /locus_tag="LGJ20_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBK2386661.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7228..8193
                     /gene="yeaX"
                     /locus_tag="LGJ20_002620"
     CDS             7228..8193
                     /gene="yeaX"
                     /locus_tag="LGJ20_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBK2386662.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLKENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8247..9362)
                     /gene="rnd"
                     /locus_tag="LGJ20_002621"
     CDS             complement(8247..9362)
                     /gene="rnd"
                     /locus_tag="LGJ20_002621"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBK2386663.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9444..11135)
                     /gene="fadD"
                     /locus_tag="LGJ20_002622"
     CDS             complement(9444..11135)
                     /gene="fadD"
                     /locus_tag="LGJ20_002622"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBK2386664.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFL
                     RIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDP
                     SLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11340..11921)
                     /gene="yeaY"
                     /locus_tag="LGJ20_002623"
     CDS             complement(11340..11921)
                     /gene="yeaY"
                     /locus_tag="LGJ20_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBK2386665.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11961..12656)
                     /gene="tsaB"
                     /locus_tag="LGJ20_002624"
     CDS             complement(11961..12656)
                     /gene="tsaB"
                     /locus_tag="LGJ20_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK2386666.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12714..14624)
                     /locus_tag="LGJ20_002625"
     CDS             complement(12714..14624)
                     /locus_tag="LGJ20_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK2386667.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14756..15100
                     /locus_tag="LGJ20_002626"
     CDS             14756..15100
                     /locus_tag="LGJ20_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBK2386668.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            15463..15822
                     /locus_tag="LGJ20_002627"
     CDS             15463..15822
                     /locus_tag="LGJ20_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBK2386669.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(15942..16121)
                     /locus_tag="LGJ20_002628"
     CDS             complement(15942..16121)
                     /locus_tag="LGJ20_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBK2386670.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16195..17556
                     /gene="pabB"
                     /locus_tag="LGJ20_002629"
     CDS             16195..17556
                     /gene="pabB"
                     /locus_tag="LGJ20_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBK2386671.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            17560..18138
                     /locus_tag="LGJ20_002630"
     CDS             17560..18138
                     /locus_tag="LGJ20_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBK2386672.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18322..19686
                     /gene="sdaA"
                     /locus_tag="LGJ20_002631"
     CDS             18322..19686
                     /gene="sdaA"
                     /locus_tag="LGJ20_002631"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBK2386673.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            19817..21415
                     /locus_tag="LGJ20_002632"
     CDS             19817..21415
                     /locus_tag="LGJ20_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2386674.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(21419..22975)
                     /gene="yoaE"
                     /locus_tag="LGJ20_002633"
     CDS             complement(21419..22975)
                     /gene="yoaE"
                     /locus_tag="LGJ20_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBK2386675.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            23438..24409
                     /gene="manX"
                     /locus_tag="LGJ20_002634"
     CDS             23438..24409
                     /gene="manX"
                     /locus_tag="LGJ20_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK2386676.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            24472..25272
                     /gene="manY"
                     /locus_tag="LGJ20_002635"
     CDS             24472..25272
                     /gene="manY"
                     /locus_tag="LGJ20_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBK2386677.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25285..26136
                     /gene="manZ"
                     /locus_tag="LGJ20_002636"
     CDS             25285..26136
                     /gene="manZ"
                     /locus_tag="LGJ20_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBK2386678.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26191..26649
                     /locus_tag="LGJ20_002637"
     CDS             26191..26649
                     /locus_tag="LGJ20_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBK2386679.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            27078..27644
                     /gene="mntP"
                     /locus_tag="LGJ20_002638"
     CDS             27078..27644
                     /gene="mntP"
                     /locus_tag="LGJ20_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBK2386680.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(27641..28450)
                     /gene="rlmA"
                     /locus_tag="LGJ20_002639"
     CDS             complement(27641..28450)
                     /gene="rlmA"
                     /locus_tag="LGJ20_002639"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBK2386681.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(28616..28825)
                     /gene="cspE"
                     /locus_tag="LGJ20_002640"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(28616..28825)
                     /gene="cspE"
                     /locus_tag="LGJ20_002640"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBK2386682.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(28838..28981)
                     /locus_tag="LGJ20_002641"
     CDS             complement(28838..28981)
                     /locus_tag="LGJ20_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBK2386683.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(29651..29938)
                     /locus_tag="LGJ20_002642"
     CDS             complement(29651..29938)
                     /locus_tag="LGJ20_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBK2386684.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDDDIIRLVAER"
     gene            complement(30013..30156)
                     /gene="mgrB"
                     /locus_tag="LGJ20_002643"
     CDS             complement(30013..30156)
                     /gene="mgrB"
                     /locus_tag="LGJ20_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBK2386685.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
BASE COUNT         7518 a         7724 c         7400 g         7611 t
ORIGIN      
        1 gattgaaaac gaaacgacat gcgcctgcga agcgcctcat ttcacactcc tgttgatcac
       61 ctggtcttaa ctggaattta aatgcctgta gtcgtttcac aggccaaatg atactttggt
      121 ctgtgaaaaa agaaaccgat atccggcgtg gaaggcattg tgttttcctg atgcatgttc
      181 acctggtctt tgtcaccaga tacagacgtc agatttttga ccatgacgcg acagaaaaac
      241 tacgcgctta cttttcaaat gtatgtgctg attttgaagc tgaacttgtt gaaatggatg
      301 gcgaaccaga tcacgtccat ttgttaatta actatcctcc caaactggcg atatccagtc
      361 tggtaaacag cctcaaaggc gtatcgggta ggtgactgcg acgagatcga ccagatattg
      421 cagtcaggta ttactacaaa ggcgttttgt ggagtgctgg ctattttgcc agtagctgcg
      481 gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca cctggtcagg
      541 tggaaaaccg cgccttatat ccctgccctg aaggatgggg ttttacggcg caccggataa
      601 gaacgcacgg tattcaccag atcttttatc acttcagccg ccacttctgg caccagcaaa
      661 gtcatcggcg tctctgtttc ataatcgaca gaaacgccat tgctgttatt ggtgacggtc
      721 acggtatacg ttgctttgcc catgattcat ttcccgttat gaatgacttt ccgttgttgc
      781 gcaccttcca tcaggacttc aggagccacg aagaagtcaa tgttgaaata agtatcgtca
      841 gtcatggctt caatgttgtg ccacttttct ggagggaaca ccgcaaactg ccccgcttcg
      901 ataaggatca cctgatcagg ctctgcactg tgttcatcag cgtagccgag atatttgacc
      961 gccccatgca taacggaaag gcgtgggtaa acccccgggc gcgttccttt atcaagatga
     1021 cgttcgaata ttccggcagg cgcagtttgt ttattccaga aaggcgttga gcgcgtatga
     1081 atataattct gtgggatttg cagcatccta ttccctcctt cggggaatgc gctgaaaacg
     1141 gtttattcca gccgtttcag ggtacgcctg ataatttgca ttttaaatac catttattgg
     1201 ttacttttta gcaccatatc agagaaaaat cagggaggat tacaagtggg gagcccgcga
     1261 caacgcgggc tgaatgtatc aggattgcag cgtcgccagt cgggcggcga aacccacgaa
     1321 catcaaacca atcagtgagt tgccaacttt agccagtttc tttttggtac gtatgtactg
     1381 cgtgacaaaa gcaccagaaa taatcaggaa gctcaaatag cagaaactca ccagttccag
     1441 cgtcgtcgcc agaataaaga atgaaattcc cgtatgtggg gcattaacat cgataaactg
     1501 tacgaaaaac gacacataga acaaaatggc tttcggatta gtcaggctca aaattaacgc
     1561 gcgtttaaaa atggccccgt attggggctc atcggatttg gcctcgttat ttttaccctt
     1621 cagggtcgcg tagagaattt tactccccag atagagcaaa taaaacgcac caagataacg
     1681 tacaatgttg aataatatcg gggtggtctt aattaatgtc gccactccag cccatgccag
     1741 aaacatcaat actgcatcgc caataaatac accgcaggcc gcaagataac cgcctttcat
     1801 accgctactg acgctatttt tgagtacaaa cagggtattt ggccctggca ccaacacaat
     1861 aaaaatggcc ccaaccagat aggtccagta attcagaacc ccgtattcag cgaacacatt
     1921 aacctcttta attatctttc gatcatgcgc gattaaaggt gaatatgcta accaatctgt
     1981 agcggcttag aaaggagaaa atcaggtttt aacccgatat caacccgata attgaatcat
     2041 taatcaggca tgctccagtg aaaaattcgg gtagtgctcg gcaaaatact ggcgtaaaaa
     2101 ttctaccgtt atccgcactt tcgctgacgt cgccagcctt gaaacataaa cggcccagac
     2161 gttcgctggc tggtaatatt ccggtagcac ttgcactaaa tgaccactgg caatgttttc
     2221 gctaacatcc caccaggagc gcagagcaat cccctgccca tcgagacacc actgatggac
     2281 aatttctcca tgattagacg acagtgggcc ggtgacttta atcgcatggt ggccttcttt
     2341 attgcgtaat tgccagacac caaaaggatg atcgcgctct ttaatgacca ggcacggcaa
     2401 tgcagaaaga tcggttaaat gtttcggcgc accatgttgc gcaataaatt ccggcgacgc
     2461 gcagagaatg cggtaattag tcgccagttt gcgggcgatt aaattaggtg cgatgtcatc
     2521 gccaatgcga atatcgagat caacaccttc attgaccaaa tcgaccaacc ggtcttccac
     2581 atcaaaacgt aattccagtt gcggatacgc cttcgccagc gctgataacg ccggagccac
     2641 cacctgtcgt ccaaaaccaa agctgctgat aatacgcagc atcccctggg gcacctgacg
     2701 cacgtcagaa agttcgtcca tcatctgacc gacatcctgc aaaatccgct gcgcccattc
     2761 ataaatccgc tctccttctt cggtaatggt gacgcggcgg gtggtgcggt gtaacaacac
     2821 cacgtttagc gtttgctcca gcaaggcgat gcgcttgctg acgaacgccg gtgaaacgcc
     2881 cagttcttcc gccacggcgg caaaaccagc ccgacgagcc accagcataa agacgcgcaa
     2941 atcattcagc agcggtaaat tattcatgat tcgtgtttta tgtttcacca gttacgggga
     3001 ttaattcctt ttcagtcaat tataggatgg tgatgttgtc aattttgatg gtcaggaagt
     3061 gagaacccaa tgatgaaaac gatgcgtatt gctgcgatcc cgggagacgg gattggcaaa
     3121 gaagtccttc ctgaagggat tcgcgtgtta caggctgccg ctgagcgctg gggcttcgac
     3181 ttgagttttg agcaaatgga gtgggcgagc tgcgagtatt acagccatca cggtaaaatg
     3241 atgccggacg actggcatga gcaacttagc cgtttcgacg ccatctattt tggtgccgtc
     3301 ggctggccgg ataccgttcc ggaccatatt tcgttgtggg gttcgctgct gaaatttcgt
     3361 cgtgaattcg accagtacgt caacctgcgc ccggttcgtc tctttcctgg cgttccctgc
     3421 ccgctggcgg gaaaacagcc tggcgacatc gatttttacg tggtcaggga aaacaccgaa
     3481 ggcgaatatt cctcgctcgg cggtagagtg aatgaaggta cagagcatga agtcgtcatt
     3541 caggaatcgg tatttacgcg tcgtggtgtc gatcgcattt tgcgttatgc cttcgaactt
     3601 gcgcaaagcc gcccacgtaa gacgctaact tctgccacta aatcaaacgg tttagccatc
     3661 agcatgccgt actgggatga gcgagtggaa gcaatggccg agaattaccc ggagatccgc
     3721 tgggacaagc agcatattga tattctctgc gcgcgttttg tgatgcagcc ggaacgcttc
     3781 gatgtggtgg tggcgtccaa tttatttggc gatatccttt ccgatcttgg cccggcctgc
     3841 accggcacca ttggcattgc cccatccgcc aacctgaatc cggaacgcac tttcccgtcg
     3901 ctcttcgagc cagtccacgg ttccgcgccg gatatctacg ggaaaaacat tgctaaccct
     3961 atcgccacga tttgggccgg tgcaatgatg ctcgattttc tcggcaatgg cgatgagcgt
     4021 ttccagcaag cgcataacgg tattctggca gcaattgaag aagtgattgc tcacgggccg
     4081 aaaacacctg atatgaaagg caatgccacc acgccacagg ttgccgacgc gatttgcaaa
     4141 attattttgc gttaaggtca aaccagttta tttgaaccgc gtcactgacg cggttttttt
     4201 attcgttctt tgcagtaaat aacctgcgtc atttcacctt ttattgtttc cgtttcgtgt
     4261 tttatggctt tccgtattct taattgttta atttatgtaa catgcaaata atcaatttgt
     4321 taaacacaac atacaactca ttaccctgca aataattaca actggcaaaa gccagagacg
     4381 ggcttttatg cccaggagat gactcatgat gagcaatgtt aagaaaaaag atgtgccgct
     4441 gataagcatc agcctggtgg ccattctttt catcgcagct gcattaagcc ttttcccaca
     4501 acaatcggcc gacgcggcca acgcaatata cacttttgtt acgcgtacgt taggttccgc
     4561 cgtacaggta ttggttttgc tggcaatggg actggtgatt tatttagcca ccagtaaata
     4621 cggcaatatt cgtcttggcg aaggaaaacc ggaatacagc acactctcct ggctgtttat
     4681 gtttatttgt gccggtttag gttcttctac gctttattgg ggggttgctg aatgggccta
     4741 ttattatcaa acgcctggat taaatatcgc accgcgttca caacaggcac tcgaatttag
     4801 cgtcccttac tccttcttcc actggggcat cagcgcctgg gcaacttata cgctggcctc
     4861 attaatcatg gcttatcact ttcatgtgcg taaaaacaaa ggtctgagtc tttccggcat
     4921 tattgccgcg attaccggcg ttcgcccgca aggcccatgg ggaaaactgg ttgatttgat
     4981 gttcctgatc gccactgtcg gcgcactgac tatttccctt gttgtcaccg cagcaacctt
     5041 tactcgtgga ctttccgcgc tgaccggttt acccgataac ttcaccgtgc aggcatttgt
     5101 gatcctgctt tccggcggca ttttttgcct aagctcgtgg attggtatca acaacggttt
     5161 gcaacgtctg agcaaaatgg ttggctgggg cgcgttcctg ctgccattac tggtgctgat
     5221 tgtcggccca accgaattta ttaccaacag catcatcaat gccatcggtc tgaccacgca
     5281 aaacttcctg caaatgagct tattcaccga tccgcttggc gatggttcat ttacccgcaa
     5341 ctggaccgtt ttctactggc tgtggtggat ctcatacacc cctggcgtag caatgtttgt
     5401 cacccgcgtt tcccgcggtc gtaagattaa agaagttatc tggggactga tcctcggcag
     5461 caccgtcggt tgctggttct tcttcggcgt aatggaaagc tatgccattc atcagtttat
     5521 caatggcgta atcaacgtcc cacaggtgct ggaaacactg ggcggcgaaa cagctgtaca
     5581 gcaagttctg atgtcgttgc cagccggtaa attgttcctc gccgcatacc tgggcgtgat
     5641 gattattttc cttgcctcgc atatggatgc ggtggcctac accatggctg cgaccagtac
     5701 gcgtaatctc caggaaggtg acgatcctga ccgtgggctg cgtcttttct ggtgcgtggt
     5761 gatcactctg atcccgcttt ccatcttgtt taccggtgct tcgctggaaa cgatgaaaac
     5821 caccgtcgtg ctcacagccc ttcccttcct cgtcatttta ctggtgaaag tcggcggatt
     5881 tattcgctgg ctgaaacagg attacgccga cattccggct catcaagttg aacattatct
     5941 cccgcagaca ccggttgaag ccctggaaaa aacgccagtg ctccctgcgg gaaccgtatt
     6001 caaaggcgac aactgagcgc catcatccta acgataaagg tatccctatg agcaatctga
     6061 gccctgactt tgtactaccc gaaaattttt gcgctaaccc gcaagaggcg tggaccattc
     6121 ctgcccgttt ttataccgat cagaacgcgt ttgaacacga aaaagagaac gtcttcgcca
     6181 aaagctggat ttgcgtcgct cacagcagcg aactggcgaa tgccaatgat tatgtgacgc
     6241 gtgagatcat tggtgaaagc atcgtgctgg tacgcggtcg tgataaggtt ttgcgcgcgt
     6301 tctataacgt gtgtccgcac cgtggtcatc agttgttgag cggtgaagga aaagcaaaaa
     6361 atgtgattac ctgcccgtat cacgcatggg cattcaaact cgatggcaac ctggcccatg
     6421 cacgtaactg cgaaaacgtc gccaatttcg atagcgacaa agcgcaactg gttccggtgc
     6481 gtctggaaga atatgccgga ttcgtcttca tcaacatgga ccccaacgcc accagcgtag
     6541 aagatcaatt acccggcctg ggcgcgaaag tgctggaagc ctgcccggaa gtccacgatc
     6601 tgaaactggc ggcccgcttt accacccgca cgcctgccaa ctggaagaac attgtcgata
     6661 actatctcga gtgctatcac tgtggtccgg cgcatccagg tttctccgac tccgtacagg
     6721 ttgatcgtta ctggcacacc atgcacggta actggacgct gcaatacggt ttcgccaaac
     6781 cgtccgaaca gtcgtttaaa tttgaagagg gtacggatgc ggcattccac ggtttctggc
     6841 tgtggccgtg cacgatgctg aacgtcaccc cgatcaaagg gatgatgacg gtcatttatg
     6901 aattcccggt ggattctgaa actaccctgc aaaactacga tatttacttc accaatgaag
     6961 agttaaccga cgagcaaaaa tcgctgattg agtggtatcg cgatgtgttc cgtccggaag
     7021 atttacgtct ggttgaaagc gtacagaaag ggctgaaatc gcgtggctat cgtggtcagg
     7081 ggcgcatcat ggccgacagt agcggtagtg gcatttccga acatggtatc gcccatttcc
     7141 ataatctgct ggcgcaggtg tttaaggact aatgacatcg gcggcggtat tttccgccgc
     7201 tgggctgatt tttgatggag tacagcaatg tcagactatc aaatgtttga agtacaggtg
     7261 agccaggttg aaccccttac cgaacaggtg aaacgcttca cgctggtggc aaccgatggc
     7321 aaaccattac ctgcgtttac tggaggaagt cacgtcattg tgcagatgag cgatggtgat
     7381 aaccagtaca gcaatgcgta ttcactactg agttcgccgc atgacacctc ttgttatcag
     7441 attgccgttc ggctgaagga aaactcgcgc ggcggttccc gctttttgca tcagcaggta
     7501 aaagtgggca atcggttaac gatttcaacg cctaataacc tgtttgcgct aattccctca
     7561 gccagaaagc atctgtttat cgcgggcggt attggtatca cccctttcct gtcgcacatg
     7621 gcagagctgc aacacagcga cgtcgactgg cagctacatt actgctcgcg aaatccagaa
     7681 agttgcgcat ttcgtgatga gctagtccag catccgcagg ctgagaaagt ccatttgcat
     7741 cattcatcaa ccggaacacg actggaatta gcgcgattat tggcggatat cgaacctggc
     7801 acacacgttt atacctgtgg ccccgaggcg ctaattgaag cggtaagaag tgaagctgcg
     7861 cgtctggaca tcgccgccga tacgctgcac tttgagcaat ttgctatcga agacaaaacc
     7921 ggcgatgcat ttaccctggt gcttgcccgt tccggaaaag agtttgtggt gccggaagag
     7981 atgactattt tgcaggttat tgaaaataat aaagccgcga aagtggaatg tttatgtcgt
     8041 gaaggggtat gcggaacctg cgaaacagca atactggaag gtgaagctga ccatcgggat
     8101 caatatttta gcgatgaaga gcgtgccagc cagcaaagta tgttgatctg ttgttcgcgt
     8161 gcgaagggta aacgcctggt gttggatttg tagtttgccg aagccggatg tggcgctgag
     8221 cgcgcccagt ccggcttcgg aagattttac tgcggatatt cctgcaataa attgtgtaat
     8281 gcttccgcca tcagctcacc acgccagccg gaaatcagct ccggcaaatt gttctgcggt
     8341 ttcagtttcc agtgccagtt cagcagttgg ttgatttgcc gacgcgatgc cagcaattcg
     8401 gcgctgatct tatgcgtttc gctcacgtca gtaatcagcg acttaatcgc tttaaacgct
     8461 ttacgataac ccggcatgtc catcaggtta agcatcggct gcggtaaggc ctcttccggc
     8521 aatgtctgcg ctttttccac cagcgccagc agcgttttac cgtgaaagcg gatttcgcta
     8581 ccggataaac ccaggctgtc cagttcgcct aaacttcccg gcatataacg cgctaccgac
     8641 cacaaatgct cttcacgcac gacaaagttc accgccagat cgcgctctcg cgccttgcgc
     8701 agtcgccagt cggctaacag ttgcagacag gccagttggc gtgtgcgtaa ttgccaggca
     8761 ttggtgatat cacgccaggc atcttccggc gcaacgactt cctgacgacg catttgcatc
     8821 aggcggcatt catccagcgc cgcaggtagc cagccggagg cctccgtttc taccataagc
     8881 ttggcggtga tcggtaacag ataccagaca tccgccgctg cgtattcaca ctgacgttcg
     8941 gtcagcggtc tggccagcca gtcggtgcgc gattcactct tgtccagcgt aacgccggaa
     9001 tactcttcca ccatggaagc gaaaccccat gacatcgggc gtccgcagaa ggcagcaagg
     9061 atttgcgtgt caatcaaggg ttgtggtaat tcgccaaaga cattgaggaa cacttccaga
     9121 tcttcactgc ctgcatggag aaattttgtg atggacggat cgcgcaggat cgctttcagc
     9181 ggtgaccagt cggtgatccc gagaggatca attagcgcca gatgctcgcc atcgaaaagt
     9241 tgaatcaacc ccagctgcgg gtaataagtg cgcgtacgaa caaattcagt atccagggct
     9301 atcgccggaa aggcacggac ggcttcacac aaagaagcca gcgcatcgtc cgtggtaatc
     9361 atttggtaat tcaaattgtt ttctctttag tgggcgtcaa aaaaaacgcc ggattaaccg
     9421 gcgtctgacg actgacttaa cactcaggct ttattgtcca ctttgccgcg cgcttcgtca
     9481 cgtaattctc gtcgcaaaat ttttccgacg ttagatttcg gtaactcatc acgaaactcc
     9541 accagcttcg gtactttgta gcccgtgagc tgacggcggc aaaaggtcac cagtgactct
     9601 tcggtaagcg atggatcttt tttcactacg aagattttca ccgcttcacc actggagccg
     9661 gaaggtacgc caacagccgc gacttcctgt acgccaggat gctgcatgac gacatcttca
     9721 atctcgttgg gatagacgtt aaaaccggaa accagaatca tgtctttttt acgatcgaca
     9781 atgcgcagga acccttcttc atccatcacc gcaatgtcgc cggtgtgtaa ccagccattt
     9841 ttgatgatct catctgtagc atccggacgc tgccagtaac ccagcatcac ctgcggtcct
     9901 ttgacacaaa gctcacccgg ttgacccggt ggtacttcat tatcattatc atcatcatcc
     9961 accagtttgg cttccgtcga cggcaccggc aaaccgatgc taccactatg ataatcaata
    10021 tcatatgggt taacgctgac cagcggcgca cactcggtaa ggccatagcc ttccagcaga
    10081 tattgtcctg tcagtttcac ccaacgctct gccaccactt gctgcactgg catccctccg
    10141 cctgcggaaa gatgcagact ggagaaatcc agctgctgga actctttatt gttcagcaac
    10201 gcattgaaca aggtgttaac gcccgtgata gcggtaaacg gatatttcgc taactctttt
    10261 accaaccctg gaatatcgcg cgggttagtg ataagcaggt tctgcccacc cagttcgata
    10321 aacagcaggc agttaatggt cagggcaaaa atgtgataca gcggcagcgc cgtcaccacc
    10381 agctctttgc ccggatgcaa cagcggacca taggtggcgt tgacctgttc caggttcgcc
    10441 agcatattgc ggtgagtcag catcgcgcct ttcgccacac ctgtggtgcc gccggtgtat
    10501 tgcaggaagg ctaaatcttc cggcaccagt tcgggtttga cgtactgcat ccggtagccg
    10561 ttatgcagtg cgctacgaaa tgaaatggca tctggcagat ggtatttcgg caccaaacgc
    10621 ttgatgtatt taacaacgaa attgactacc gtgccttttg ccgtagatag ctgatcgccc
    10681 atacgggtca gaattacgtg ctgaacggcg gttttatcaa ccactttttc cagtgtgtga
    10741 gcaaagttag acacgataac aatcgccgat gcgccgctat cgttaagctg atgctcaagc
    10801 tcacgcgggg tatacaacgg gttaacgttt acgacgatca tcccggcacg caaaatgcca
    10861 aacagcgcca ccggatattg caataaatta ggcatcatca acgcaacgcg atcgcctttc
    10921 ttcagcccca acccttgttg caaataagcg gcaaacgcgc gactgcgttc ttccagcttg
    10981 cggaaggtca ttacctcccc catattcaca aacgcaggct gatcggcgta gcgcgcgacc
    11041 gactgctcaa acatatctac cagagattga taacggtcag ggttgatctc cgtcggaacg
    11101 tccgcgggat aacggttaag ccaaaccttc ttcaattctt cacctctaaa atgcgtgttc
    11161 gtcgtcatcg caaccccaaa tgatatacat gccgttaaca taatattaac tcatcatacc
    11221 agcttgataa ttacccaacg aaaaggttgc gaagcgcgtc actatttatt tttatcttta
    11281 ccgtaagaat gcagaaacag cggaccagcc gctgtttctt tttctttaaa aacaagcaat
    11341 tattcagtta caactgtttg tactctcgcg gggccgggat tataccagcc ccatccgcca
    11401 tagccatagg gccagccacg accgccataa aaccatggat caatcggctg aggcggcata
    11461 atcacctgct gggttaaatg ccaacgtttg taacccgtta cttgcatcac cataaattta
    11521 tagggcgtgt tgccgatttt tccgtcaacc gcaccagtga ttggcccgac taccgtaacc
    11581 agttgtccac ggaaatccac cgggtccaga aaaccgttca tatcggcata aatgcgaccg
    11641 cgagaaggtt ctcccagcgt cggtctggct ccgctgtcca gcggtacggt agcaatttcc
    11701 aggcgggttt tcccttgctg gttttgtacc gcaaccactt tgccaccaaa gcgtgcctcc
    11761 tgaccaacgt acagctgcgg cgcactcatc acccgaacta aatcttgttg cggcgtggga
    11821 ctgctgcctt taatggcgtc cggcacagtg acacaaccgc tcagcattag cgcaaacgta
    11881 cctgccagta tgcctttgat aacattcttt tgaaccgcca tggtgcgact cctttttttc
    11941 tcagggcata ctcttaagat tcattctttg cccggaagtt tcttccatgc gacgttgtta
    12001 cgtaaataaa ccggttcggc atgttccacc gccaccgttt taccctcggc aaacatctga
    12061 cacgcaatcg gcagcatatc ttccgccgct ggcagtaaaa cttctccgtc gcgcaaaacc
    12121 agcccgctct ctttaccgag atccggccag gcttgccagc ccgtccctac cgttacccat
    12181 tcgccggaaa gctgttgcat tcgttcatga acgagttcgg gtttgagtac ggcttcggtt
    12241 tcttcaccgc gccagatacc gttttcatca cgctgatatt cggcccagta aacttcgccc
    12301 attcgcgcgt caatggctga cagcacgcgg gttgcgccgt ttttgcgcca cgccccttgc
    12361 gccatcgtca ttagcgtgga gacgccaatc atcggtaatt ccgcgccaag cgccagccct
    12421 tgcgcgatgc caataccaat gcgcacgccg gtaaagctac cggggccgcg cccgtaagcc
    12481 agagcgttta tgtcagtcag ggaagttccg ctggtggtca ggatatcctg caccatcggt
    12541 aagattcgtt gagtatgttc acgagggcaa agctcaaaat gagcgttgac agtaccgtcg
    12601 ttccacaggg cgacagagca ggcctctgtc gcggtatcga tagccagaat tcgcatgggt
    12661 cttcgtgctt ttttcaataa aaaggcgcgc atcataccat actccgtaac aaattacctg
    12721 gaggatggta tcgcaaggaa acgaaccgca cgggcaatgt cacgagtgcg tggcgcgggc
    12781 ggcagactgg cgagaaacgt cgcgccgtaa ggacgcatca ccagccgatt gtcacaaatc
    12841 accagcacgc cacgatcgtc ggcgtcgcga atcagtcgcc ctaccccctg tttgagagta
    12901 atgacggcat ctggtagttg cacttcatcg aacgggtcgc caccgcgcaa acgacaatct
    12961 tccatgcgcg cttttaacag tggatcatcc ggcgaggtaa acggcaactt gtcgataatc
    13021 accaatgaca atgtatcgcc acgcacgtcc accccttccc agaaactact ggttgccaca
    13081 agaagcgcgt taccggcgct gacaaattgc tgcaacagtt gccctttgct ggtttccccc
    13141 tgcaacaaaa cgggaagcgt catggtagcg cggaactgct cggccagatc acgcatcatg
    13201 gcgtgcgagg tacaaagcat aaaacaacga ccgttgttag cttcgatgat cggtcgcagc
    13261 attgccgcta actggcgagc agaacctggc tggttggttt gcggcagatt gcgcggcaca
    13321 cagagtaacg cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt
    13381 tctatcccaa gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag
    13441 gtgaagatcc agctaccagg cttttgcgcc attaactctt tgaatttatc cgccacgctg
    13501 agcggcgtga gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc
    13561 ggctgattga tctcttttag ccgcttcagc cgtgtgcgat acaacgtagc gcgctcaaat
    13621 gccgcatcca gcaaggcgga acgccccagt gacagtttcg ccacgtcata acaaagttcc
    13681 agggtgtcat cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc
    13741 aggttaccgc gataacctgg ctcaccgagt tgcagacgaa aatcctgcgc actctgggca
    13801 agacgatcag cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaagcgatg
    13861 gtgatgtctt ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg
    13921 ctggcaatgt ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttccgggatc
    13981 agttcgccaa atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc
    14041 accaccacat cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac
    14101 atcgggcagt cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgt
    14161 gaatcttccg ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag
    14221 gagcgcagca ggatcacatc gcttaagatt tgtaccggca gatcgccccc cgccagcgcc
    14281 tgctgttcga gacgttcgag gcagaggtag tttgagcgcc ctttcagcag cgccacgttg
    14341 cccgtatatt tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc
    14401 aacgcttttg agccggtcga gataatgact ttctttttcg cccgcagcgc aggagccagg
    14461 taagcgtagg ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct
    14521 atcgcctggg tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt
    14581 atcgctttcg ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat
    14641 ttgaacaggg attatgtcag gatgagggcg ctttcgccag ttgaagtagt gacggcgacc
    14701 tcacattgtg gcagtctttg cagcacgaaa atggaaggat aatgaggaca aaatgatgac
    14761 tatcgttcgt atcgatgctg aagcccgctg gtctgatgtg gtaatccaca acaacacgct
    14821 ctactacact ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac
    14881 gctggcgcag attgatgccg tgctggaaaa acagggcagc aataaatcga gcattctgga
    14941 tgccaccatt ttcctggccg ataaaaacga cttcgcggcg atgaataaag cgtgggatgc
    15001 ctgggttgtc gcgggtcatg cgccggtgcg ctgcacggta caagcgggtt tgatgaaccc
    15061 gaagtataaa gttgaaatta agattgtggc tgcggtgtaa gctttatcga agcaaaataa
    15121 gtcagacgat aatttatcga taatactggt cggttttaca taaatcgacc agagattaag
    15181 atattacctg ttgatgctcc tgctaattca ttatttattt ttataatttc aattttatct
    15241 ataaaactaa ctatataaat aaccataacc atgccgttgt gttcgccctc tgactagcaa
    15301 ctctggtttt ctttcttagc tgaataatct ggttatagcg tgaggactga ccgttaaatt
    15361 tctccatctt actggataac acaccatgag ctgctacctt taaaacacct gaatgtcaac
    15421 aggttaactc gtgccatatc gttataatcc ctttgatatt agatgcaaat taaggtcata
    15481 tatagtctta tcgataacat ggttaatttt aaggataaga atatgcctgc tgtaatagat
    15541 aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag ttcgatgaat
    15601 gaaagcacag cgaagggaat ctttaaatat ttaaaagaac tgggagtacc cgcgagtgcc
    15661 gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggttcac ggaaaaaatg
    15721 gttggatggg caaaaaaaat ggagtctggt gaacgcactg taattaaaaa tcctgaatac
    15781 ttttcaacat atatgcagga agaactaaaa gcactggtct gagcttaatt tatatcagca
    15841 taaatgggtc aggacgcttt taatcacata taaaaagcgt cctttttctc acaatcaaat
    15901 aatcatcgga ttctataacg aaatcgatac ggattagtgt gttactcgtc ttcatcctca
    15961 aaacgcgcca cgattcgctc gccggagtgg ttggcacgca gctcttctgc caccagcgcg
    16021 attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc gacagctttt
    16081 tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc ctgctaaatt
    16141 gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg actgatgaag
    16201 acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga attttatttc
    16261 tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc cgatcatccg
    16321 tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac tttcggtaaa
    16381 gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc gctacaggtg
    16441 ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga tttgccattt
    16501 cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga gtcactgcca
    16561 gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta cgattgggcg
    16621 ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga tgtcaatgcc
    16681 cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac gctcacttcc
    16741 gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca ggtacaggaa
    16801 tatctgcaca gcggtgattg ctatcaggtg aatctcgccc agcgttttca tgcgacctat
    16861 tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc gccatttagc
    16921 gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg atttattctt
    16981 tgtgataata gtgaaatcca gacccgcccg attaaaggca cgctaccacg cctgcccgat
    17041 cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga tcgtgccgaa
    17101 aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt agccggttcg
    17161 gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca tctggtcagc
    17221 actataacgg cgcgactacc agaacagtta cacgccagcg atctgctgcg cgcagctttt
    17281 cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat cgacgaactg
    17341 gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt ttgcggcaac
    17401 atggatacca gcattactat ccgcacgctg actgccatta acggacaaat atactgctct
    17461 gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga aacttttgat
    17521 aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg tagcctgacg
    17581 cttgatgatt ttttatctcg ctttcaactt ttgcgcccgc aaattaaccg ggaaacccta
    17641 aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc ggggttgttg
    17701 ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt ccctggaggt
    17761 gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc tgaagaagag
    17821 gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt cgatagcgta
    17881 actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc gtatcgcgcc
    17941 agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt acatctgggt
    18001 cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg gctgtcctgg
    18061 tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct ggcgctgcaa
    18121 attggtgtga aaccctgact atacttatct ttacatctac aaaacactac ttgagacaat
    18181 catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga atagttaagc
    18241 cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg gaagtccagt
    18301 caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt ggggattggt
    18361 ccctcatctt cccataccgt agggcctatg aaggcgggta aacagttcgt cgatgatctg
    18421 gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta tggttcactg
    18481 tcgctgacgg gtaaaggcca ccacaccgat atcgccatta tcatgggtct tgcaggtaac
    18541 gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt agaagagcgc
    18601 gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga caacgggatg
    18661 cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca cgcctataac
    18721 ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt tatcgtcgat
    18781 gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc gttcaaatct
    18841 gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg tctcgctatg
    18901 cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc gcatgtctgg
    18961 caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact gccaggcccg
    19021 ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag cgataaactg
    19081 tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc agttaacgaa
    19141 gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg cggtatcgtt
    19201 ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga catctatacc
    19261 cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc ctctatttcc
    19321 ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc tgctgcgggt
    19381 cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga aattggcatg
    19441 gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt gccgtgcatt
    19501 gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc tctgcgccgc
    19561 accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga aaccggtaag
    19621 gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa agtccagtgt
    19681 gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc cgctttctcg
    19741 tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt cgccatacgc
    19801 ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc gccgtaatcg
    19861 aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga gcgtgcgatt
    19921 tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc acgctgtaga
    19981 ggagttagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc ttccgctgat
    20041 tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa aactccaaac
    20101 tgtgcgatcc attggcctgg tgcaagacgg cacactctat tgctccagca tttttggtta
    20161 tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac cacttttacg
    20221 cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat ggacgcctgc
    20281 agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac tgacggcaat
    20341 gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg acaaacggca
    20401 tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca atgaaaacta
    20461 tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg gggctacggc
    20521 gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa gtttactggt
    20581 gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg aaatcaatct
    20641 gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg cgcgcagcca
    20701 gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg gctggatttc
    20761 accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac tgacccgcta
    20821 tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc agtttcatgt
    20881 tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag atctcaatca
    20941 gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg aacgcgatgc
    21001 cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg tcaaactggc
    21061 gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat tacgtcctga
    21121 cgtgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg ttaactcgac
    21181 ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg tggcggaggg
    21241 cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata ttttgcaagg
    21301 gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg cgggcagcca
    21361 accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac gttaatgctt
    21421 actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca acacgataat
    21481 cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg cccacacggg
    21541 gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca tcgtcatcgg
    21601 caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg ccttttacca
    21661 gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg aattcacccg
    21721 caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca aactcgttgg
    21781 tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg atcggatcga
    21841 gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca tcaacgccct
    21901 cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca tccagttcac
    21961 cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg atttcatcga
    22021 ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc ccgcgcagag
    22081 aacgcgacgc cagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg gcaaatgcac
    22141 cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg gcctgacgtt
    22201 tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc aaagtcgact
    22261 ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg atcgagaagc
    22321 caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct gccaccagac
    22381 tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg tgctggttaa
    22441 cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc actaccgccg
    22501 ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca tccaacgaga
    22561 agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg tagcctttac
    22621 cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt gttgctttga
    22681 acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag gagaaatcca
    22741 tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac agcagcccca
    22801 gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt tgttttggcg
    22861 gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc acaatttcga
    22921 gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat tccatgacaa
    22981 gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa cgacaaaatg
    23041 caaacgaaag gtgcggcata gagagccaga aaggtaggcg ttaaaaggcc tgatgctgaa
    23101 atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg ttgttaaacg
    23161 ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc tttgcaaacg
    23221 aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta aattacggat
    23281 cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg caggggtttt
    23341 tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa atacatctgg
    23401 cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta ttgttatagg
    23461 cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt taggcgagca
    23521 ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc tgattgaaaa
    23581 gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc tcgttgatac
    23641 atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag agcattatga
    23701 agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggccc gtgatgatga
    23761 cccaagcttt gatgaactgg tggcactggc agtagaaaca ggccgtgaag gcgtgaaagc
    23821 tctgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag caccaaaagc
    23881 ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg gccttgcgcg
    23941 tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag aaaccaatgt
    24001 ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta agacactgct
    24061 cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca aaatgattcg
    24121 cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta ccaacccaac
    24181 agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg tcggtggtat
    24241 ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg aaaaagatat
    24301 cgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta aggtttccac
    24361 cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac gtattgtgtt
    24421 gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac aatggagatt
    24481 accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat gggatcaatc
    24541 ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg tatcgttctt
    24601 ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc gctgggctgg
    24661 atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat ttctaccatt
    24721 ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat ccctctggcc
    24781 gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt ccagcacgct
    24841 gcggataagg ctgctgataa cggcaacctg acagcgattt cctggatcca cgtttcttct
    24901 ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct gtctgttggt
    24961 accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa tggtctgaat
    25021 atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat gatgcgtgct
    25081 ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac caactttaac
    25141 ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca acttagcccg
    25201 aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga tctcgataac
    25261 gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac cgagaaaaaa
    25321 ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca gggttcatgg
    25381 aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc aattcgtcgc
    25441 ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct ggagttcttt
    25501 aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct ggaagaacag
    25561 cgtgctaatg gcgcagagat cgacgacggt gctatcaacg gtatcaaagt cggtttgatg
    25621 gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc ggtatttgca
    25681 gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct gttcttcatc
    25741 ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg ttactccaaa
    25801 ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac ggaaggggcg
    25861 tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca tgtcaacatc
    25921 ccgctggttg tctctcgcat tactgaccag acgggcaaag aacacgttac tactgtccag
    25981 actattctgg accagttaat gccaggcctg gtaccactgc tgctgacctt tgcttgtatg
    26041 tggctactgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt cgtcatcggt
    26101 atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact accggggcct
    26161 tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt actgattctt
    26221 ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc ccgccgtaac
    26281 ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag cgttatcttc
    26341 gtcggattga ttgtcattct tatctataac aacgtcacga atcatggcgc gctaataaca
    26401 acatggttat taagtgcact ggctctgatg ggtttttata tattctggat ccgcgttccg
    26461 aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga atatagccga
    26521 atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga acagcgtcgg
    26581 ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct tctcgtatca
    26641 actcaataac ttatgaattt agccaaagct atgtttagtg tatttttaat aatcagacat
    26701 agcttaggct atattatctc ttcccttatt tgttatttat tttaacgttt cattgatata
    26761 taaatctaaa taaaaatcgt tatcaataaa gcaatgaaat aatatattcc aacagttgtt
    26821 ttataatctc aaaatatgtt aaggttgcgc cctcatttgg ggagtagctg atttccagat
    26881 tccggaaatg tacgtgtcaa catactcgtt gcaaaacgtg gcacgtacgg actgaagact
    26941 ttcagtcagg cgagaccata tgcacatcaa tcgctatgcc tgcatgaacg cgaaaaccat
    27001 cgcggcttgt tgttcatgta tgcatgtttg ctgggggcga tgatgtgttt tatggatacc
    27061 ccggtcagga cgttgtcatg aatatcactg ctactgttct tcttgcgttt ggtatgtcga
    27121 tggatgcatt tgctgcatca atcggtaaag gtgccaccct ccataaaccg aaattttctg
    27181 aagcattgcg aaccggcctt atttttggtg ccgtcgaaac cctgacgccg ctgatcggct
    27241 ggggaatggg catgttagcc agccggtttg tccttgaatg gaatcactgg attgcgtttg
    27301 tgctgctgat attcctcggc gggcgaatga ttattgaggg ttttcgtggc gcagatgatg
    27361 aagatgaaga gccgcgccgt cggcacggtt tctggctact ggtaaccacc gcgattgcca
    27421 ccagcctgga tgccatggct gtgggtgttg gtcttgcttt cctgcaggtc aacattatcg
    27481 cgaccgcatt ggccattggt tgtgcaacct tgattatgtc gacattaggg atgatggttg
    27541 gtcgctttat cggctcaatt attgggaaaa aagcggaaat tctcggcggg ctggtactga
    27601 tcggcatcgg cgtccagatc ctctggacgc acttccacgg ttaataagaa cgctgccaga
    27661 ggtgaatatt aaagtccgtc tggcagtcga acacttcttt tgctgccagt gtttgccaga
    27721 cttctggctt cgcacgccag gcaaacggcg tcatctgcaa taatgcgacg gcttcatcac
    27781 cgcgaagacg catcggataa cacaactcat cactctgctg taatgtaaaa ccttccagtt
    27841 gttctgcatg aggtgcatga agatgtactt cattgtaaat cagccccttc agctccatca
    27901 aatgtcgcgg tcccggcgtg gcagtaatga cccagccgcc gggcttcact actcgtgcta
    27961 attcttctgc tttacacggc gcgtaaatac gtattatggc gtccatactg gtatcggaaa
    28021 acggcaaacg gtggctggaa gcgacacaaa gagtgacctg cggatagcgt ttcgccgccg
    28081 cttttatcgc taccttcgag acatccaggc caaacgtggt gatttcgggc aacgcatcgg
    28141 caaatgcgtg tgtgtaatac ccttcaccac agccaatatc cagcactgcc gtggcctttt
    28201 catcaagccg ttcccgcagt tgggcgacaa ttgcatcacg cagcggctga taatgtccgg
    28261 catctaagaa tgcgcggcgt gcttgcatca tttccgcgct gtcgcccgga tcacgagacc
    28321 gtttatgctg aacgggcagc agattgacat acccttcttt cgccatatca aactgatgtc
    28381 gctggggaca gatatagctg tttttttcac gcgaaagagg ctgatggcaa agtggacagg
    28441 aaaacgacat gacaactccg gcaggatact aaaggccgca agtgtaacgc gaatcacgcg
    28501 gcgggcaaat atccgaacgc caggagcgaa gataaagcat agtaaaaagc ctcgcattcg
    28561 acgaggcttt atagcgagat tgaagcgtat tcacacttca gatcagtgga ttcgatcaga
    28621 tagctgttac gttaacagct gccggacctt tctggccgtc ctgaatttcg aactcaacgt
    28681 tctgaccttc agccagagtt ttgaagccat taccctggat agcggagaag tgtacgaaca
    28741 cgtctttgct gccatcagcc ggagtaatga agccaaaacc tttagactcg ttgaaccact
    28801 taacctgacc tttaatcttt gccatttgaa aaattcctta gattgttttc ttcgcccgca
    28861 ggcataacat agataaaact gacacattac tgcatgaggc accaatataa ggctcggcag
    28921 agaagcggta ttcaacgtca acgtgtttac tcaggacttc tttactgaaa atgccacaca
    28981 taaacagaac tgtacctcgt ttaacccgaa atctgttatc acatacaacg ttaattatgg
    29041 caagccattt ttaaacatgt ctcgatcaga cgcacaaatc tcgatacgtc ttccactttt
    29101 tttgcacact tatgcaacgg aatacgcgcc gatatatcat tgtgcttaac cttgccagtt
    29161 caggcagata cttaaactgg cgtagtttct aacatagttc aataagttca gttgttccag
    29221 gccagtggga gaagttatta catagtgcgt gcaatatcac attttctgct atgcaatgaa
    29281 taaaaagtta tatcactttt tctcataaaa cagtcagtta acggctatca attaccctaa
    29341 agagaagtca atccccaaag ggattgtaaa tttaaaataa gaaaaattga tgagtgagca
    29401 aaaaaatcaa gagagaaacg tttcgctaat aaataatacc acacttaatc acaaatccga
    29461 tgtaaacatc ctaataaatt aatgaggaaa atatcttttt ctgatatccc gttacgcaaa
    29521 taaataatat gataattatt tgaagtcaga ccagagtttt aatttgaagc agttgcacca
    29581 tgatagtgcg tataatcagg atgaattgag taacccttca acgacggggt tactcaagat
    29641 tgaggaagga ttatcgttcg gcgaccagac gaataatatc gtcgtcttca accgattttt
    29701 gactctcaat ggtcttttta tctgctttct ctggttcgtt tgcttcgcac aaacgacgta
    29761 acagtgcatt ttgacgtttt tgctgatcca gcaacgcctc aagcagttca atctgttcgt
    29821 tagtccgaga actggcacga ttaataaaaa accacaagat gagcccgata agaagaacca
    29881 ccaccgatac aaccaaagac gcaatgttca tcacgcctga atttacaact tcgttcattt
    29941 caccacctct gagtagaggc gctattctac cactgctgga gaggaagaaa atctagtgct
    30001 gaaaaaatga tatcaccacg ggataaactg gttaatggca caaattccgc tgaaaaattg
    30061 tacatcctga tcgcacatca tgttgaatac ctgcgcccaa agcagcaagc aagccaacac
    30121 cacgacaacc agaacgaccc atcgaaactt tttcactcca atctccgttt cacctacctt
    30181 atgtcattaa actagcatgc gttagttaaa cagcgcctaa ctatgtcgat attcgtgctt
    30241 tttcggaatg agt
//
LOCUS       DAFATA010000049        29348 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFATA010000049 DAFATA010000000
VERSION     DAFATA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.050"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(107..483)
                     /gene="rnpB"
                     /locus_tag="LGJ20_002644"
     ncRNA           complement(107..483)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LGJ20_002644"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(516..1661)
                     /gene="garK"
                     /locus_tag="LGJ20_002645"
     CDS             complement(516..1661)
                     /gene="garK"
                     /locus_tag="LGJ20_002645"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBK2386686.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1758..2648)
                     /gene="garR"
                     /locus_tag="LGJ20_002646"
     CDS             complement(1758..2648)
                     /gene="garR"
                     /locus_tag="LGJ20_002646"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBK2386687.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2678..3448)
                     /gene="garL"
                     /locus_tag="LGJ20_002647"
     CDS             complement(2678..3448)
                     /gene="garL"
                     /locus_tag="LGJ20_002647"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBK2386688.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3464..4798)
                     /gene="garP"
                     /locus_tag="LGJ20_002648"
     CDS             complement(3464..4798)
                     /gene="garP"
                     /locus_tag="LGJ20_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBK2386689.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5173..6744
                     /gene="garD"
                     /locus_tag="LGJ20_002649"
     CDS             5173..6744
                     /gene="garD"
                     /locus_tag="LGJ20_002649"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBK2386690.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6893..7228
                     /gene="prlF"
                     /locus_tag="LGJ20_002650"
     CDS             6893..7228
                     /gene="prlF"
                     /locus_tag="LGJ20_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBK2386691.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7228..7692
                     /gene="yhaV"
                     /locus_tag="LGJ20_002651"
     CDS             7228..7692
                     /gene="yhaV"
                     /locus_tag="LGJ20_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBK2386692.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRFFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7747..8556)
                     /gene="agaR"
                     /locus_tag="LGJ20_002652"
     CDS             complement(7747..8556)
                     /gene="agaR"
                     /locus_tag="LGJ20_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBK2386693.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8805..10085
                     /gene="kbaZ"
                     /locus_tag="LGJ20_002653"
     CDS             8805..10085
                     /gene="kbaZ"
                     /locus_tag="LGJ20_002653"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBK2386694.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            10108..10581
                     /gene="agaV"
                     /locus_tag="LGJ20_002654"
     CDS             10108..10581
                     /gene="agaV"
                     /locus_tag="LGJ20_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBK2386695.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10592..11371
                     /locus_tag="LGJ20_002655"
     CDS             10592..11371
                     /locus_tag="LGJ20_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBK2386696.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11361..12239
                     /locus_tag="LGJ20_002656"
     CDS             11361..12239
                     /locus_tag="LGJ20_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK2386697.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12257..12691
                     /locus_tag="LGJ20_002657"
     CDS             12257..12691
                     /locus_tag="LGJ20_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK2386698.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12688..13821
                     /gene="nagA"
                     /locus_tag="LGJ20_002658"
     CDS             12688..13821
                     /gene="nagA"
                     /locus_tag="LGJ20_002658"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK2386699.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDIHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14171..15325
                     /locus_tag="LGJ20_002659"
     CDS             14171..15325
                     /locus_tag="LGJ20_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBK2386700.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15338..16198
                     /gene="kbaY"
                     /locus_tag="LGJ20_002660"
     CDS             15338..16198
                     /gene="kbaY"
                     /locus_tag="LGJ20_002660"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBK2386701.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16365..16841
                     /gene="agaB"
                     /locus_tag="LGJ20_002661"
     CDS             16365..16841
                     /gene="agaB"
                     /locus_tag="LGJ20_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBK2386702.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16880..17683
                     /gene="agaC"
                     /locus_tag="LGJ20_002662"
     CDS             16880..17683
                     /gene="agaC"
                     /locus_tag="LGJ20_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBK2386703.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17673..18464
                     /gene="agaD"
                     /locus_tag="LGJ20_002663"
     CDS             17673..18464
                     /gene="agaD"
                     /locus_tag="LGJ20_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBK2386704.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18465..19220
                     /locus_tag="LGJ20_002664"
     CDS             18465..19220
                     /locus_tag="LGJ20_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBK2386705.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            19620..20204
                     /locus_tag="LGJ20_002665"
     CDS             19620..20204
                     /locus_tag="LGJ20_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2386706.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            20284..20979
                     /locus_tag="LGJ20_002666"
     CDS             20284..20979
                     /locus_tag="LGJ20_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2386707.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            21009..23525
                     /locus_tag="LGJ20_002667"
     CDS             21009..23525
                     /locus_tag="LGJ20_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2386708.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDAKGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGEIATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            23536..24627
                     /locus_tag="LGJ20_002668"
     CDS             23536..24627
                     /locus_tag="LGJ20_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2386709.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFNITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(24670..25530)
                     /gene="rsmI"
                     /locus_tag="LGJ20_002669"
     CDS             complement(24670..25530)
                     /gene="rsmI"
                     /locus_tag="LGJ20_002669"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK2386710.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            25594..27630
                     /gene="lpoA"
                     /locus_tag="LGJ20_002670"
     CDS             25594..27630
                     /gene="lpoA"
                     /locus_tag="LGJ20_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBK2386711.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGCTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            27588..27983
                     /locus_tag="LGJ20_002671"
     CDS             27588..27983
                     /locus_tag="LGJ20_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBK2386712.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            28003..28593
                     /gene="diaA"
                     /locus_tag="LGJ20_002672"
     CDS             28003..28593
                     /gene="diaA"
                     /locus_tag="LGJ20_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBK2386713.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            28603..29178
                     /gene="yraP"
                     /locus_tag="LGJ20_002673"
     CDS             28603..29178
                     /gene="yraP"
                     /locus_tag="LGJ20_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBK2386714.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
BASE COUNT         7355 a         7527 c         7385 g         7081 t
ORIGIN      
        1 actgaccgat aagccgcttt cttttgggta tagcgtcgtg gacagtcatt catctttccg
       61 ctcctccaaa agcaaaaacc cgccgaagcg ggtttttacg taaatcaggt gaaactgacc
      121 gataagccgg gttctgtcgt ggacagtcat tcatctaggc cagcaatcgc tcactggctc
      181 aagcagccta cccgggttca gtacgggccg taccttatga acccctattt ggccttgctc
      241 cgggtggagt ttaccgtgcc acggactgtt accagccgcg cggtgcgctc ttaccgcacc
      301 ctttcaccct tacctgatcc cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact
      361 ggtcgtgggt ttccccccca ggcgttacct ggcaccctgc cctatggagc ccggactttc
      421 ctcccctccg cccgtctccc ccgaagagga cgacgacgaa gcggcgactg tctggtcagc
      481 ttcggcgcgc agtatagagg gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc
      541 gccagtgtcg cggcgatatt acgtgaagca cggcagatat tgtcataagc cccgcggaat
      601 gcttcgtcca acgtacctat gctggtcaat acgctgaaga ccgcatcgat gccatgctga
      661 tgtacaacgc caacatcatc ggtcaggcta cccgcaatgc caatcaccgg tttatggtac
      721 ttcttcgcca cgtttgcaac gccaatcggt accttgccgt gaatactctg gctgtcaata
      781 cgcccttcac cggtgatcac cagcgtacaa tcgtgaatat gttcctccag attcagcgcc
      841 gtagtgacga tttcaatacc acttttcagt tccgcaccaa gaaacgccat cagcgccgca
      901 cccataccgc ccgcagctcc tgcgccgggg acatctttca catcaacatg cagcgctttt
      961 ttaatgacat cggcatagtg agagaggtta ttgtccagct caacaatcat cgcttcactg
     1021 gctccctttt gtgggccaaa gatgcgcgat gcgccgttat cgcccaccag cggattggtg
     1081 acatcacaag cgacgcgaat gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca
     1141 atatcattca gagtattaag actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg
     1201 cataatttcg cccccagcgc ctgtaccatg cctgcgccgc catcatttgt agcgctaccg
     1261 ccaatgccga taataatgtt tgtcgcgccg ctctccagcg cctgcaggat taactcgcct
     1321 gtgccgcgtg aagtggtcac gagtggatcg cgtttttccg caggtaccag ctccagccca
     1381 ctggccgccg ccatttcaat aaacgcggtt ttgccatcgc cggagatccc ccaactggca
     1441 ttcactttct cgcccagcgg ccctgtaacc caggcgtgac gttcagaacc ctgggtggct
     1501 gcaatcatcg cttccaccgt tccttcgcca ccgtcggcaa ccggaataga aacgtactgt
     1561 gcatcaggaa aaatttcccg aaatcctttt tctatcgcct gcgcaacctc gctggcagat
     1621 aaactttctt tataagagtc tggggcgatt acgattttca tacctatgcc tgttaccaca
     1681 tgacgccgga gggcgtttct cttattcggc ctggattcca ggcccggatt gcaatacgcc
     1741 atccgggcac gacgtcatta acgagtaact tcgactttcg ccagtttttc gtagtagcac
     1801 gccagggcgc tatgatccgc cgttcctaaa ccatctgctc gcagtgcctg catcatctcc
     1861 ataaccgcag ctgtgagcgg cagttgcgcg ccgacgccgt gggaagtatc cagcgcattt
     1921 gccagatcct taatatgcag atcaatacgg aagcccggct tgaagttgcg gtccatcacc
     1981 atcggcgctt tggcatccag cacggtactg cccgccagtc caccgcgaat tgcctgatac
     2041 accaggtccg ggttaacgcc cgctttggtt gccagcgtta gcgcttctga catcgcggca
     2101 atattcagcg ccacaatgac ctgatttgcc agtttggtga cgttacctgc accgatttcc
     2161 ccggtatgca ccacggaacc cgccatcgct ttcatcaaat catagtattt gtcgaaaata
     2221 gccttgtcgc cgcccaccat cactgacagc gtaccgtcga tggctttcgg ttcaccgccg
     2281 ctcaccggag catccagcat atcaatgcct ttcgctttca gcgcttcgct gatttcacgg
     2341 cttgccagcg gtgcgataga actcatatcg atcaataccg tacctggctt cgcgccttca
     2401 ataatgccat tctcacccag cgccacctct ttcacatgag gggagtttgg cagcatggtt
     2461 atgatgacgt cgcactgttc agcgatcgct ttagccgtag acgctgtttc tgcacctgca
     2521 gcaatcacgt cagcaatagc ttctgggtta cggtcagcaa ccaccagcga gtaacctgct
     2581 ttcagaaggt ttttactcat tggtttaccc ataatcccca ggccaataaa accaactttc
     2641 atagtcatat caatcatctc tcttgttgcg gtggtggtta ttttttaaag gtatcagcca
     2701 gtttctgagt ggcagagcgg aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc
     2761 cccattccag ataacgacgc gcatcggctt cgaccggcgc gagaataccg ctgggtttgc
     2821 cgtgcgcact ggcacggtta aaaatgtgct gaattgcttt ttgtacatcc gggtgcgagg
     2881 cattgccgag atggcctaat gccgcagcca gatcgctggg gccgacgaag ataccgtcta
     2941 caccttcggt agcggcaatg gcatcgacgt tatctacgcc ctgctgactt tctatctgga
     3001 ccagaatggt gatgttcttg ttcgactgag cgaaataatc cgccacggtg ccaaacatat
     3061 tggcgcggtg agaaacggag acgccgcgaa tgccttccgg tgggtaacgg gttgatgcca
     3121 ccgcctgctc tgcttcctct tttgtttcta caaaaggaat caggaagtta tagaaaccga
     3181 tatccagaag acgcttaata attaccggct cgttggtcgg cactcgcact actggcgcgc
     3241 tggcgctgcc tttcagggcc attaactgcg gaataaacgt ggagatatcg tttggcgcat
     3301 gttcgccatc cagcaccagc cagtcaaacc cagccaaacc aagaacttca gtgctaatcg
     3361 ggttagagag tgctgaccag caaccaattt gtacctgttt cgcagccagt gcggctttga
     3421 atttattcgg gaaaacatcg ttattcatcg cttatacctt tgcttatttc tgcaattcca
     3481 tacgtttaat gtcgccaact acgaagaggt agcagaccat cgccatcagc gctgaacatc
     3541 ccacgaaaac cagtgctgca ttgaaggagt gcagttcact taccaggtag ccaatcacca
     3601 gtggagtgac aatggaggca acattgccaa agacgttaaa gacgccgcca cagaggccaa
     3661 caatctcttt cggcgcggtg tcagaaatca ccggccagcc cagagcacca aatcctttgc
     3721 caaaaaaagc cagcgccatc agcatgacca ccagcgtggt gttgttggtg tagttacata
     3781 agatgatggt ggaagccagc aacattccca gcacaatcgg tagcttacgt gccagggtca
     3841 gggataaacc gcgtttgatc agataatccg agaagacacc tcccagcacg ccgcccgcaa
     3901 aaccacacag tgccggaatc gaggcgacca gacccacttt cagaatcgac atgccttttt
     3961 cctgcaccag ataaatcggg aaccaggtga ggaagaacca ggtgatggtg ttgataaaat
     4021 attgtccgaa aaatacgccc aacatcatgc ggttagagag caattgcttg atgtaatgca
     4081 gtttgggacc gcttgctgcc gcgctgcccg gctttttgtg gtccatatcg accaccgcgc
     4141 cattttcaga gataaacttc agctcttccg cagacatacg tgggtgatct gtcgggttat
     4201 gaatcaattt gatccacagc gccgtcagca caaaaccaat cacccccata acggtaaaga
     4261 cgtgctccca gccccaggcg aaagtcagcc agccaagcag aggcgaaaag agcgccagcg
     4321 agaaatattg cgccgagtta aagatggcgg aggcagtacc acgttctttc gtcgggaacc
     4381 aggcggcgac aatacgggcg ttcgccggga atgatggcgc ttccgagaag ccgagcataa
     4441 agcgcataaa gaacatggag atccctgccc aggccagcgg gaacatatca acaaagcctt
     4501 gcaggaaagt gaatagcgac cagaaaaaga ggctataggt ataaactttt ttcgagccaa
     4561 acttatcaag cagccagccg ccggggattt gcatcagcaa gtaggcccag ccaaaagcag
     4621 aaaagatgta acccatcgaa accgcactta actgcaactc ttttgccact tcggtaccag
     4681 caatagacag cgttgcacga tcggcgtagt taacggcggt aacaataaaa ataatcagta
     4741 atattaaata gcgggtatgc acgcctttct ttttttcgtc aacggtgtcc agaatcattt
     4801 tatttacctc gggtacttat gctgattttt attattatgg ggaaggtgtt atttatgagt
     4861 ttcatttatg ccgtaacggc aatgaactcg ggaattagta taagcagcgc gagaataata
     4921 atcattgtgc aaatgctaat ttaattaata ctatttaaat attattttga gcatatgcac
     4981 ataaggttgc gcgctaaagc acagatttgc gctttacctt gccgggcggc actgcaatcc
     5041 ctgaaatgat tgacattgat cacatttctg cgtttaaact cctgacattc ttatttcacc
     5101 caatgaagtc atttattttt aaatgagacc aggtcctcat tttaataacc cctggctgga
     5161 gaatattgca caatggccaa catcgaaatc agacaagaaa cgccaactgc gttttatata
     5221 aaagttcacg acacagataa tgtggcaatt attgttaatg ataatggcct gaaagcagga
     5281 acgcgttttc cggatgggct ggaattaatt gaacatattc cccaggggca taaagtcgca
     5341 ttgctggaca ttccggctaa tggtgaaatt attcgttatg gcgaagtgat tggttacgcc
     5401 gtgcgtgcaa tcccacgcgg aagctggatc gacgaatcaa tggttgtact accggaagcg
     5461 ccgccgttac acacgctgcc actggcaacc aaagtcccgg cccccttacc gccgctggaa
     5521 ggatacacct ttgagggcta tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc
     5581 ggcatcacca ccagcgtcca ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt
     5641 gaacgcgatc tgctaccgaa atacccgaac gtcgatggcg tggtggggct gaatcatttg
     5701 tacggctgtg gcgtggcgat taacgcaccg gcggcagttg tgcctattcg taccatccac
     5761 aatatttcgc tgaaccctaa ctttggcggc gaagtaatgg tgattggtct gggttgtgaa
     5821 aagttgcagc ctgagcgcct gctggtcggc acggatgatg tgcaagctat tccagtagaa
     5881 agcgccagca ttgtcagttt gcaggatgaa aagcatgtcg gttttcagtc catggtcgag
     5941 gatattttgc aggtcgccga acgccatcta caaaaactga atcaacgtca gcgagaaacc
     6001 tgtccggctt cagaactggt tgtcggcatg cagtgcggtg gcagcgatgc cttttctggc
     6061 gtaacggcaa acccggcggt tggctatgcg tctgatctac tggtgcgctg cggcgcaacg
     6121 gtgatgttct cagaagtcac ggaagtgcgt gacgcgatcc atctgctgac accacgcgca
     6181 gtgaacgaag aggtcggcaa acggctgctg gaggagatgg agtggtacga taactatctc
     6241 aatatgggaa aaaccgaccg cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg
     6301 gcaaacgtgg tagagaaggc actcggctcc attgctaaat cgggtaaaag cgcaattgtt
     6361 gaagtgctgt cgcccggtca acgcccgact aaacgcggat taatttacgc cgcgacgcca
     6421 gccagcgatt ttgtctgtgg cacgcaacag gtggcttcgg gtatcaccgt gcaagtgttt
     6481 acgaccggtc gtggtacgcc gtacggcctg atggcggtac ccgtcattaa aatggcgacc
     6541 cgcaccgagc tggcgaaccg ctggtttgat ttaatggata ttaacgcggg cactatcgcc
     6601 accggcgaag aaaccattga agaggtgggc tggaagttgt tccactttat tctcgacgtc
     6661 gccagcggga agaagaaaac cttctcggat caatgggggc tgcataacca gctggcagtg
     6721 tttaacccgg caccggtgac ctgatttctt ttggaacgtc cctcgcaaaa catggcctta
     6781 gtgccatgtt tttattgttt aaagccccca cgtccattaa taatgcattt gcattacctt
     6841 agttcaagct tataatttga gcagaaaaca ggctgtaaaa ggacagtgaa tcatgcccgc
     6901 taatgctcgc tctcacgctg tactgaccac tgaatcaaag gtcacgatac gcggacaaac
     6961 aactatcccc gcgccagtgc gtgaggcctt aaaactgaag ccaggccagg acagcattca
     7021 ttacgaaatt ctgcctggtg ggcaagtctt tatgtgccga ctgggagatg aacaggagga
     7081 tcatactatg aatgcatttt tgcgttttct ggatgcagat atccagaaca acccgcaaaa
     7141 aactcgtcca ttcaacattc aacaaggaaa gaaacttgtc gctggcatgg acgtcaacat
     7201 tgatgatgag attggcgacg acgaataatg gattttccac aaagggttaa tggttgggcg
     7261 ctatatgctc atccctgttt tcaggaaacc tacgacgctt tagttgccga agtcgagaca
     7321 ttaaagggaa aagatcctga aaattatcag agaaaagccg ccacaaagtt attggcggta
     7381 gtccataaag tgattgagga gcatatcacg gtcaatccat catcaccggc attccgtcat
     7441 ggcaagtcgt taggctctgg gaaaaataaa gactggtcac gggtaaaatt tggtgctggt
     7501 cgttatcgtt tcttctttcg ttatagtgaa aaagagaaag tcatcattct gggatggatg
     7561 aacgatgaaa acactctgcg cacctacggt aaaaaaacag atgcctatac cgtattcagc
     7621 aaaatgttaa aaagaggaca tcctcctgcc gactgggaaa gcctcaccca agagacagaa
     7681 gaaaaccatt gatggtgttc acaatgcccg ccgaacgtat tcgtgttgac cagcgggcac
     7741 cctttctcac tccccgacca gaatcacttc aaccccagcc tttcgcagtc cttccagact
     7801 atccgcagga atgccttcat caacaatgat catgtcgata cgttgagtat caatgatctt
     7861 atgtaaactg gaacgattga atttactgga atcggtgacc acgatgatcc gttccgcaac
     7921 ttcgcacatc cgacggttta aacgggcttc atcttcatta tgtgtgctga cgccgcgctc
     7981 cagatcgatc gcatctacac caagaaacag catatcgaag tggtaatttt gcagcgattg
     8041 ctcagcctga tcgccgtaaa aagattgcga ctgacggcgc aaatgcccgc cggtcatcag
     8101 cagctcaacg ccttccgctt ccagcaacgc attagccacg ttcataccgt tggtcatcgc
     8161 aattacgtca gtgtgcttac gcatcagacg agcaatctca aaagtggtag tcccggaatc
     8221 gaggatcacc cgatgaccag gctgaatcaa ctcaacggca gctttcgcaa cgctgcgttt
     8281 catcgcggtg ttcagcgcgc ttttatcttc cactgatggc tcgactgacg gcgtcgtgct
     8341 atcgcagatc aacgcgccac cataggcacg cacagcgatc ccctgctttt ccagaaacgc
     8401 cagatcattg cggatcgtca cagtagatac gccatacaat gccgacagat cgttaacctg
     8461 cacactccct tgctgtcgca gacgctgaat gatctgttct cgtcgctcgc tggtgccagt
     8521 cactcgcttc tcacctgaag cgtcggtatt actcatagta agtcctttcg taaaactttc
     8581 gtttcatttc gttttgccta ttaacgcctt tctattaagc aaatgcaagc ccaccttgcc
     8641 cattggcgca agctactctc gtttcagtga ctttcattat gtttcttttg tgaatcagat
     8701 cagaaaacta ttatctttcg ttttattttt atctcaccat gacgcagtat caactgaaac
     8761 aaaacgaaag attaatatcg cagtaatctg aactggagag gaaagtgaaa catctgacag
     8821 aaatggtgag acagcacaaa gcgggcaaaa caaatggaat ttatgccgtt tgttccgcac
     8881 atccgctggt gctggaggct gcaatccgct acgccagtgc aaaccaaacg ccgttactga
     8941 ttgaagcaac ctccaatcag gtagaccagt tcggcggtta taccggaatg acgcccgccg
     9001 attttcgcgg ctttgtttgt cagctcgccg actcgttgaa tttcccgcag gatgcgttga
     9061 ttctgggtgg tgaccatctg gggccaaacc gctggcaaaa cctgccggcc gctcaggcaa
     9121 tggccaatgc cgatgatttg attaaaagct acgttgcggc aggattcaaa aaaatccacc
     9181 ttgattgcag catgtcctgt caggacgatc cgattccctt aactgatgac atcgtggctg
     9241 aacgcgccgc ccgtctggcg aaagtggcgg aagaaacctg tcttgaacac tttggcgaag
     9301 ccgatctgga gtatgtcatt ggtaccgaag tgccggtacc tggcggcgcg catgaaacct
     9361 taagcgagct ggcggtcacc acgccggatg ccgcccgcgc cacgctggaa gcccatcgtc
     9421 acgcctttga aaagcaaggt ttgaatgcca tctggccacg catcattgcc ctggtggttc
     9481 aacccggcgt cgaattcgat cacaccaacg ttattgatta tcagcccgcc aaagcgagcg
     9541 ccttaagcca gatggtcgaa aactacgaaa cgctgatttt cgaagcgcac tctaccgatt
     9601 atcaaacgcc gcaatcgctg cgccagctgg tgattgacca ctttgccatt ctgaaagttg
     9661 gcccagcgct gaccttcgcc ctgcgtgaag ctctgttctc tctggcggcg attgaagaag
     9721 aactggtgcc agcgaaagcc tgttctggtc tgcgtcaggt gctggaagac gtgatgctcg
     9781 accgcccgga atactggcaa agccactacc acggtgacgg caacgcgcgt cgtctggcgc
     9841 gtggttatag ctactcggat cgcgtgcgct attactggcc ggacagccag attgatgacg
     9901 ctttcgctca tctggttcgt aatctggcgg attcaccaat tccgctaccg ctcatcagcc
     9961 agtatctgcc gctgcaatac gtgaaagttc gctccggcga gctgcaaccg acgccacggg
    10021 aactcattat caaccatatt caggacatcc tggcgcagta ctacacagcc tgtgaaggcc
    10081 aataagcaaa acaaagagga acacgctatg ccaaatattg ttttaagccg gattgatgaa
    10141 cgcttgattc acggtcaggt cggcgttcag tgggtcggat ttgcgggggc aaatctggtg
    10201 ctggtagcca acgatgaggt tgccgaagat ccggtacaac aaaacctgat ggaaatggta
    10261 ctggcagaag ggatcgccgt acgtttctgg acgctgcaaa aagttatcga caacattcat
    10321 cgcgccgccg atcgacagaa aatcctgctg gtttgtaaaa cacccgccga tttcctgacg
    10381 ctggtgaaag gtggcgttcc ggtgaatcgc attaacgttg gcaatatgca ctacgccaat
    10441 ggcaaacaac aaatcgccaa aacggtttct gtggatgcgg gcgatatcgc agcatttaac
    10501 gacctgaaag ccgctggggt ggaatgcttc gttcagggcg tcccgacaga gcctgctgtg
    10561 gacctcttta aattactttg agggattcat catggaaatc agcctgttgc aggcatttgc
    10621 gttgggcatt atcgccttta tcgctggcct ggatatgttt aacggcctaa cacatatgca
    10681 ccgcccggtg gtcctcggcc cgttggtcgg gctggtactt ggcgatctgc ataccggaat
    10741 tttaaccggc ggtacgctgg aactggtgtg gatggggctg gccccgctgg cgggcgcaca
    10801 gccgcctaac gtgattatcg gtactatcgt cggcacggcg tttgccatta ctactggcgt
    10861 gaaacccgat gtcgcggtcg gtgtcgccgt acctttcgct gtcgcagtac agatggggat
    10921 taccttcctg ttctcggtga tgtctggcgt gatgtctcgc tgcgaccgga tggcggagaa
    10981 cgccgatact cgcggcattg aacgcgtgaa ctatctggca ctgctggcac tcggcacttt
    11041 ctattttctc tgcgctttcc tgcctatcta cttcggcgcg gaacatgcaa aaaccatcat
    11101 tgatgtcctg ccgcaacgat taatcgacgg cctcggcgtc gcgggcggca tcatgccagc
    11161 aatcggtttt gccgtgctgc tgaaaatcat gatgaaaaac gtctacatcc cctacttcat
    11221 cctgggcttt gttgccgcag cctggctcaa gttaccggtg ctggctattg ctgccgctgc
    11281 gctggcgatg gcgctgatcg acctgctgcg taaatctcct gaaccgactc aacctgcggc
    11341 acagaaagag gaatttgaag atggcatcta atcaaaccac cctgccgaac gtcagtgaaa
    11401 acgaagaaac attgctgact ggcgtcaatg aaaacgtgta tgaagatcag agcattggcg
    11461 cggagctgac gaaaaaagat atcaaccgcg tcgcctggcg ttccatgctg ttacaagcgt
    11521 cttttaacta cgaacgtatg caggcttctg gctggttgta cggtctgctg cctgctctga
    11581 aaaagatcca cactaataaa cgcgacctgg cgcgcgccat gaaggggcat atgggtttct
    11641 tcaataccca tccgtttctg gtgacatttg ttatcggcat tatccttgcg atggagcgtt
    11701 ctaagcagga cgttaacagt attcagagca ccaaaattgc cgtcggtgcg ccgctcggcg
    11761 ggattggcga tgcaatgttc tggctaacgc tactgccgat ttgtggcggg attggagcca
    11821 gtctggcatt gcaaggctcc attcttggcg cagtcgtctt tattctgctg ttcaacgtgg
    11881 tacacctggg gctgcgtttt ggtctggcgc attatgctta ccgcatgggc gtggcggcga
    11941 ttccactcat taaagcgaat accaaaaaag tcggccatgc tgcgtctatc gttgggatga
    12001 cggtaatcgg cgcgctggtg gcaacctatg ttcgtttaag caccacgctg gaaatcacag
    12061 cgggcgacgc agtggttaag ttacagactg atgttattga caaactgatg cctgccttct
    12121 taccgctggt ctacaccttg accatgttct ggctggtacg ccgcggctgg agtccgctgc
    12181 gtctgattgc agttaccgtg gttctcggca tcgtcggtaa attctgccat ttcctttaaa
    12241 tacaatgagg tattagatgt taagtattat tttgacaggg catggcgggt ttgccagcgg
    12301 catggaaaaa gcaatgaagc aaattctcgg tgagcaatcg cagtttatcg ccatcgattt
    12361 tccggaaacc tcaagcaccg cgctgcttac atcgcagctt gaagaggcca tcgcccaact
    12421 ggattgtgaa gacggcattg tttttctgac ggatttgctg ggcggcacgc cgtttcgcgt
    12481 agcttcaacg ctggcgatgc aaaaaccggg ctgtgaagtg atcaccggca ccaatttgca
    12541 actgctactg gagatggtgc tggagcgcga agggttaagc ggtgaagaat tccgtgtaca
    12601 ggcgctggag tgtggacacc gagggctgac cagtctggtg gacgagttag gccgctgcca
    12661 tgaagaatgt ccggtcgagg aaggaatatg acacacgttc tgcgcgccag aaggctgctg
    12721 actgaagagg gatggctcga tgaccatcag ttgcgtattg ctgacggtgt catcgcagca
    12781 atcgaaccga ttccagtggg cgtgactgaa cgcgatgcgg aactgctctg ccccgcttac
    12841 atcgacatcc atgtacacgg tggtgcgggc gttgatgtaa tggatgacgc gccggatgta
    12901 ctcgacaagc tggcaatgca caaggcacgc gaaggtgtcg gcagttggtt gccgactacc
    12961 gtaaccgcgc cgcttaacag cattcatgcg gcgctgaaac gtattgctca acgttgccaa
    13021 cgcggcggac ctggtgcgca agtgctgggg agttatctcg aaggaccgta cttcacgccg
    13081 cagaataaag gcgcgcatcc gccggaattg tttcgcgagc ttgaaattgc cgagctggat
    13141 caattgattg ccgtttctca gcacacctta cgcgtggtag cgctggcacc ggaaaaagag
    13201 ggggcattgc aggccatccg ccatcttaaa cagcaaaatg tacgagtgat gctggggcat
    13261 agcgcggcga cctggcaaca aactcgcgcc gcgtttgatg ctggtgccga cggcctggtg
    13321 cattgctata acgggatgac aggtttacat caccgcgaac cgggaatggt tggcgcggga
    13381 ttaacggaca agcgcgcctg gctggaactg atagccgatg gtcatcatgt gcatccggcg
    13441 gcgatgtcgc tgtgttgttg ctgtgcaaaa gagagaatcg tgctgatcac cgacgcgatg
    13501 caggcagccg ggatgccgga tggtcgctat acgttatgtg gcgaagaagt gcagatgcac
    13561 ggtggcgttg tccgtaccgc gtccggtggg ctggcgggca gtacgctgtc tgttgatgcg
    13621 gcagtgcgca acatggtcga gttgacgggc gtaacgcctg cggaagccat tcatatggca
    13681 tcgctgcatc cggcgcgaat gctgggtgtt gatggtgttc tgggatcgct taaaccgggc
    13741 aaacgcgcca gcatcgttgc gctggatagc gggctgcatg tgcaacaaat ctggattcag
    13801 agtcaattag cttcgttttg atagtttgct cctttattgg gccttcactt cccccgtaag
    13861 gcctttcttt ttctttcgtt ttgatctgtg cagcggtgtc ggatgcgacg ctaacgcgtc
    13921 ttatgcgacc tacggtacgc cgcatccggc aatcggtgaa catagcgacg tcattacgca
    13981 attcttaatc ttcccttgtc ttttctttca acgatcacag atttcgtttt atttcctttt
    14041 tctccattga actttcagtt tcttttctat agattttaat caacgaaaga catcaccaag
    14101 tgaaatgaaa cgaaaggcaa gtgaaagcga caacgcccga cgtcaagttc atcagactaa
    14161 ggattgagtt atgccagaaa attacacccc tgctgccgcc gcaaccggta catggactga
    14221 agaagagatc cgccatcagc ctcgcgcatg gatccgttca ctcaccaaca tcgacgcgct
    14281 acgttccgcg ctcaataact tccttgaacc gttactgcgc aaagagaatc tgcggatcat
    14341 cctgaccgga gccggaacgt cggcatttat cggtgacatc atcgcgccgt ggctcgccag
    14401 ccataccggt aaaaacttca gcgccgtacc gaccaccgat ctggtcacca atccgatgga
    14461 ctacctgaat ccagctcatc cgctgctgtt gatctccttc ggtcgatccg gcaacagccc
    14521 ggaaagcgtc gctgccgtgg aactggcaaa tcaatttgta ccggaatgct atcacctgcc
    14581 gatcacctgc aacgaagcgg gcgctcttta ccaaaacgcg atcaacagcg ataacgcatt
    14641 tgccctgctg atgcccgcag aaacgcacga tcgcggcttt gcgatgacca gcagcattac
    14701 caccatgatg gccagctgcc tcgcggtttt cgcacctgag acgatcaaca gccaaacctt
    14761 ccgcgacgtg gcggatcgtt gccaggcgat cctgacctca ctgggcgatt tcagcgaagg
    14821 tgtgtttggt tacgcaccgt ggaaacggat cgtttatctc ggcagcggtg gcttacaggg
    14881 cgcagcacgc gagtcggcgc tgaaagtgct ggaactgacg gcgggtaaac tggcggcctt
    14941 ttatgattct ccaaccggat tccgtcatgg accaaaatcg ctggtcgatg acgaaacgct
    15001 ggtggtggta tttgtctcca gccaccctta cacccgtcag tatgatcttg atctgctggc
    15061 tgaacttcgc cgtgacaacc aggcaatgcg tgtaatcgcc atcgccgcgg aaagcagcga
    15121 catcgtcgct gccggtccac atatcatcct gccaccgtca cgtcacttta tcgacgttga
    15181 gcaggcattt tgcttcctga tgtacgccca gacgtttgca ctgatgcagt cgctgcacat
    15241 gggcaatacg ccggataccc catcagccag tggcaccgtt aaccgcgtgg tgcaaggcgt
    15301 aatcattcat ccgtggcagg cataagagga tcgcattatg agcattatct ccactaaata
    15361 tctgttacag gacgcccagg ccaatggcta cgcggtgcct gcttttaaca ttcataacgc
    15421 cgagacgatc caagcgatcc tcgaagtgtg cagtgaaatg cgatcgccgg tgatcctcgc
    15481 cggaacgccg gggaccttta aacatatcgc gctggaagag atctacgccc tgtgcagcgc
    15541 ctattccaca acctacaaca tgccactggc gctgcatctc gaccaccacg aatcgctgga
    15601 tgatattcgc cgtaaagtcc acgcaggtgt gcgcagtgca atgatcgacg gcagccactt
    15661 cccgtttgcc gagaacgtga agctggtgaa atcggttgtt gacttctgcc actcacagga
    15721 ttgcagcgtg gaagcagaac tgggccgcct gggcggtgtt gaagatgaca tgagcgttga
    15781 cgccgaaagt gcattcctga ccgatccaca agaagctaaa cgctttgtcg aactgactgg
    15841 cgtcgacagc ctggcggtag cgattggtac ggcgcacggc ttatacagca aaacgccgaa
    15901 gattgatttc cagcggctgg cggaaattcg tgaagtggtg gatgttcctc tggtgctaca
    15961 tggtgccagc gatgttccag atgaatttgt ccgtcgcact attgaacttg gcgtcacaaa
    16021 agtgaacgtt gccacagaat taaaaatagc cttcgctggc gcggttaaag cctggtttgc
    16081 ggaaaatccg cagggtaatg atcctcgtta ttatatgcgc gtcggaatgg atgcgatgaa
    16141 agaagttgtc agaaataaaa ttaatgtctg tggttcagcg aatcgaattt cagcataatc
    16201 attagttttt tatgatttat cccaatgtac ttcccgatta atatcgggga gtgccttaat
    16261 ggaaaaggag atgactaaac cttaataaat acatcagtac aatatcgcaa caataatata
    16321 ttaaaaaaaa tcatattatt caactttatg gtgaggatta cacaatgagc agtccaaata
    16381 ttctcttaac ccgtattgat aaccgtctgg ttcacggtca ggttggcgtg acctggacat
    16441 ccaccatcgg tgcgaatctg ctggtagtcg tggatgatgt tgtcgctaac gatgatattc
    16501 aacagaaatt aatgggtatt accgcagaaa cctacggctt tggcattcgt ttctttacta
    16561 tcgaaaaaac cattaacgtc atcggcaaag ctgcaccgca tcagaagatc ttcctgattt
    16621 gccgtacgcc acaaacggta cgtaaattgg tagaaggtgg cattgacctg aaagatatca
    16681 acgtcggcaa tatgcatttc tcggaaggga aaaagcaaat cagcagtaaa gtttatgtcg
    16741 atgaccagga tctcgcggac ttacgtttta ttaaacaacg tggcgtgaat gttttcattc
    16801 aggacgtccc tggcgatcaa aaagaacaaa tccctgacta aatctaaaat cgccttaata
    16861 ttggtttgag gtaataaaaa tgcatgaaat aaccctactt cagggattat ccctagcggc
    16921 gttagttttc gttctgggga ttgatttttg gctggaagcc ttatttttat tccgcccgat
    16981 aatcgtttgt accctaactg gcgctattct cggtgatatt cagactggct taattaccgg
    17041 tggtctgaca gagttggctt tcgccggatt aacccctgca ggtggtgttc agccgcccaa
    17101 cccgattatg gcgggtctga tgaccaccgt cattgcatgg tctacgggcg ttgatgccaa
    17161 aacagcaatt ggtcttggcc tgccgtttag tttgttaatg cagtacgtca ttctgttctt
    17221 ctattccgct ttctcattat ttatgaccaa agccgataaa tgcgcgaaag aggcggatac
    17281 ggcagcgttt tcccggctta actggacaac gatgctcatc gtcgcttcag cgtatgcggt
    17341 gattgctttc ctctgtactt acctggcaca aggggcgatg caggcgctgg tgaaagcgat
    17401 gcccgcctgg ttgacccacg gctttgaagt ggctggcggt attctgcctg ccgttggttt
    17461 tggcttgctg ctgcgcgtaa tgttcaaagc gcaatatatc ccttacctga tcgccggttt
    17521 cctgtttgtt tgctacatcc aggtcagcaa cctgttgccg gttgccgtac tgggcgcagg
    17581 ctttgcggtg tatgagtttt tcaatgcgaa atcccggcag caagcgcaac cgcagcccgt
    17641 tgccagtaaa aatgaagaag aggactacag caatgggatc tgaaatcagt aaaaaagata
    17701 tcacccgtct gggctttcgt tcgtcgctgc tgcaagcgag ctttaactac gaaaggatgc
    17761 aggcgggcgg ttttacctgg gcgatgttgc cgatcctgaa aaagatttat aaggacgaca
    17821 aaccgggctt aagcgcggcg atgaaagata acctcgaatt tattaatacc cacccgaatc
    17881 tggtcggatt cctgatgggg ttattaattt cgatggaaga aaaaggagaa aaccgcgaca
    17941 ccattaaagg cctcaaagtg gcactgtttg gcccaatcgc cgggattggc gatgcgattt
    18001 tctggtttac tttgttgccg attatggcgg gaatttgctc atcatttgcc agccagggaa
    18061 acctgctggg gccgattcta tttttcgccg tttacctgct tatctttttc ctgcgcgtcg
    18121 gctggaccca cgtcggttat tcagtcggcg tgaaggcgat cgataaagtg cgagagaact
    18181 cgcagatgat tgctcgctcg gcaaccatcc tcgggatcac ggtaatcggc gggctgatcg
    18241 cttcgtatgt gcatattaac gtggtgacat cgtttgccat cgacagtacc cacagcgtcg
    18301 cgctgcaaca ggatttcttc gataaagtct tcccgaacat tttaccgatg gcctacaccc
    18361 tgctgatgta ttacttcctg cgggtgaaaa aagcgcatcc ggtgctgtta atcggcgtga
    18421 cttttgtgct ctctattgtt tgttccgcat tcggcatttt gtaaatggaa cgaggcactg
    18481 cttctggcgg tgcctcttta cttaaggaat ttcatcctgt gcaaaccctt cagcaagttg
    18541 aaaactatac ggcgttaagt gaacgagcca gcgaatattt attggctgtg atccgtagca
    18601 aaccggatgc cgtgatttgc ctggcgaccg gagccacgcc attactgacg tatcattatc
    18661 tggtagaaaa aatccaccag cagcaggttg atatcagcca gctcaccttc gtgaagctcg
    18721 acgaatgggt ggatctgcca ttaacgatgc ctggcacctg cgaaactttc ctacaacagc
    18781 atatcgtgca gccgctgggg ctgcgtgaag accagctcat cagctttcgc tccgaagaga
    18841 taaatgagac agagtgcgaa cgggtaacga acctgattgc gcgcaaaggc ggtctggatt
    18901 tatgcgttct aggattgggg aaaaacggtc atcttgggct gaacgaaccg ggagaaagcc
    18961 tacaaccggc ctgccatatc agtcaacttg atgccagaac acagcaacat gagatgttaa
    19021 aaaccacggg tcgccccgtg actcgtggga tcaccttagg cctgaaggat attctcaatg
    19081 cccgcgaggt tttgttattg gtgactggcg aaggaaagca ggatgcgaca gaacgttttc
    19141 tcacggctaa agtctccacc gctatcccgg cttcgttttt atggctgcac agtaacttta
    19201 tttgtttaat taatacctaa ccagatgaaa atttgctcaa aatttttaaa ttaacttatt
    19261 caacagtcac gcattatatt aaataacatt tgactgggtt gaacataacg ccaatagcaa
    19321 aaggagttat gttcaacaat ttaattttag atatacatat aacttgttct attaataaat
    19381 cacctcgtat tctgactcgg cccctggcgc agcaaagtca ggcatttata ctctttttaa
    19441 cacatagaaa attcatatca atataataaa aacatagaat atttgcatat aaatataatc
    19501 ttaaagttta gtctatttaa tgttcaatga aatatttcta cctgtataat ctttaaagat
    19561 gttgaacata tattcacatt aaatatgatt atgtacttgt tacaaggata aggttataaa
    19621 tgaataaagt tacaaaaaca gctattgtgg gcttacttgc gcttttcgca ggtaatgccg
    19681 ctgcaaccga tggtgaaata gtttttgatg gcgagatttt aaaatccgct tgtgaaatca
    19741 atgactctga taagaaaatt gaagttgctc ttggtcacta taatgccgaa cagtttcgtt
    19801 ctgttggcga tcgtagccca aaaattccat tcactattcc tttagttaac tgcccagtga
    19861 ctgggtggga gcacgataac ggcaacgttg aagcgtcttt ccgtttatgg ctggaaacgc
    19921 gggataatgg caccgtccct aacttcccta atctggcaaa agtgggctct tttgctggca
    19981 cagcggcaac aggtgtgggt atccgtattg acgatgcgga aagcggaaac cttatgccac
    20041 tgaatgctat ggggaatgat aacacggttt atcagatccc agcggactcc aatggtattg
    20101 tcaatgttga cctcatcgct tactacgtat caaccgttga agcatcagaa atcaccccag
    20161 gggaagcaga cgctgtcgtt aacgtaacgc tggattaccg ttaatacgtt acggcgttat
    20221 ctgacctttc agataacgcc cttttccttc ctctttctcg ttgtatcagg ttgaaaaata
    20281 actatgtcaa aacgaacatt cgcggtgata ataaccttgt tgtgtagctt ctgtatcggc
    20341 caggcgcttg cagggggtat cgttttacag cgaacgcgag tgatctatga tgccagccgc
    20401 aaagaggctg cgttacctgt cgcaaacaaa ggcgcagaaa cgccttattt actgcaatca
    20461 tgggtagata atatagatgg tacaagccgt gccccattta ttataacccc accgctattt
    20521 cgtcttgaag ctggcgatga ctcatcactg cgaattatta aaacagctga taacctgcct
    20581 gaaaataaag agtcgctgtt ctacattaat gttcgtgcca ttccagcaaa gaaaaaatca
    20641 gatgatgtta atgctaacga gttgacgctg gtatttaaaa cacggatcaa aatgttttat
    20701 cgccccgcac acctgaaggg acgggtaaac gatgcgtgga catcactgga atttaaacgt
    20761 agtgaccatt cactcaatat atataaccca actgaatatt acgtcgtatt tgccggactg
    20821 gcagtcgata aaaccgatct cacaagcaaa attgaatata tcgcgcccgg agaacataaa
    20881 cagttaccac ttcctgcatc tggcggaaag aacgtgaaat gggctgcaat caatgattat
    20941 ggcggcagtt ccgggacaga aactcgtcca ctgcaataac aaatataaaa aacacaggtc
    21001 atcagggaat gccacaacga caccaccagg gacataaacg cacaccgaaa cagttggcgc
    21061 tcatcatcaa acgctgtttg ccgatggtgc tcactggcag cggcatgctt tgcactaccg
    21121 ctaacgccga agagtattat ttcgacccca ttatgctgga aactacaaaa agtggtatgc
    21181 aaacaactga tctgtcacgt ttttcaaaaa aatacgcaca actaccagga acttatcagg
    21241 ttgatatctg gctgaataaa aagaaggttt cacagaaaaa aattacattt accgccaatg
    21301 cagagcaact tctgcagcca cagtttacgg tagaacaact acgtgagctg ggtattaagg
    21361 tggatgaaat cccggcgctg gctgaaaaag atgacgatag cgtgatcaac tcgcttgaac
    21421 aaatcattcc cggtacagct gctgaatttg atttcaatca tcagcgactt aatttgagca
    21481 ttccccaaat tgcactgtac cgtgatgcaa aaggttacgt ctccccttct cgttgggacg
    21541 atggtatacc aacgctgttt accaactact cgtttacagg ttctgataac cgttaccgcc
    21601 agggcaatcg tagccaacga cagtacctga atatgcaaaa tggtgctaat tttggcccct
    21661 ggcgattacg taactattct acgtggacac gcaacgatca ggcgtcaagc tggaacacta
    21721 tcagtagtta tttacaacgt gatatcaagg cgttgaagtc tcagttgctt ctgggagaaa
    21781 tcgccaccag cggcagtatt ttttccagct acacctttac tggcgtgcaa ctcgcttccg
    21841 acgataatat gttgccaaac agccagcgcg gatttgcccc aacggtacgc ggtatcgcaa
    21901 acagtagtgc aatcgtgact atcaggcaaa atggttatgt gatctatcaa agcaacgtgc
    21961 cagcgggtgc ctttgaaatt aacgatctct acccctcttc caacagcggc gatttagaag
    22021 tcacgattga agaaagtgac ggtacgcaac gtcgctttat ccagccttat tcttcattac
    22081 ccatgatgca gcgacctggg catctaaaat atagcgcgac cgctggacgc tatcgcgctg
    22141 atgcaaacag tgatagcaag gaacccgaat ttgctgaagc cacggcaata tatggtttga
    22201 ataatacttt tacgctgtat ggcggcctgc tcggttctga agattattat gcgctgggga
    22261 tcggtatcgg cggcacactt ggcgcactgg gcgcgttgtc gatggatatc aacagagctg
    22321 acacccaatt cgataaccgg cactcttttc atggctatca atggcgtacg cagtacatca
    22381 aagatatccc ggaaaccaac accaatatcg ctgttagcta ctatcgctat accaacgatg
    22441 gctattttag ttttgatgaa gccaataccc gtaattggga ctataacagt cgccaaaaaa
    22501 gtgaaattca attcaacatc agccagacaa tatttgatgg ggtaagtctg tatgcctccg
    22561 gttcgcagca agactattgg ggcaataacg ataaaaacag gaatatctca gttggggttt
    22621 ccggtcagca atggggaatt ggttacagcc tgaattatca atacagtcgc tacacggatc
    22681 aaaataatga ccgcgcactc tctttgaatc tcagtattcc gttagaacgc tggttaccgc
    22741 gtagccgggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa catgaaatgc
    22801 gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa caaagtctgg
    22861 atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct tatggcacct
    22921 tcagtgccgg atacagctac ggtaatgaca gcagccaata caattacggc gttaccggcg
    22981 gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac gcttttgcgc
    23041 ttatcgatgc taatggggct tctggcgtga ggatacaaaa ctatccgggg attgctactg
    23101 atccctttgg ctatgcagtg gttccttatc tcacgactta ccaggaaaac cgtctctcgg
    23161 tagatactac gcagctgccc gataacgtcg atcttgagca aacaacacag tttgtggtgc
    23221 ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat cgcgtacttg
    23281 ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc agcaacgatg
    23341 agacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct gggatatcga
    23401 gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa tgtcagtttg
    23461 cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcacg gcgcagtgcc
    23521 attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt gatatccaca
    23581 tcctgcgctt atgcctcctc aggagggtgt ggagctgaca gcactagcgg tgcgacaaat
    23641 tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac aggacgggag
    23701 tttacctctg ccacgctaag tagcactaac tggcaatacg cctgtacctg ctctgcgggt
    23761 aaggcagtta aacttgtcta tatggtcagc cctgtactta ccaccactgg acatcaagca
    23821 ggatattaca aactcaatga tagtctggat attaaaacca cattacaggc gaatgacatt
    23881 ccaggactcg tgactgacca gaccgtttct gttaacaccc gattcacaca gataaaaagc
    23941 aacaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac gtctcgttat
    24001 ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa gccatttctc
    24061 ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcatg ggtcgatgga
    24121 atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat tcagggaaat
    24181 ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaggt taattttgga
    24241 ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt tactccagta
    24301 gactttaata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc gttgcaaatg
    24361 cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag acgaagaagt
    24421 tcagacaatg cgcccgatgt cggtattcgt attgaaaatc tcggcggcgg agttgcaaat
    24481 attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac catcaacatg
    24541 cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt tcagggcacc
    24601 gccaccatta ccgtcatcgt gcggtaaaag aagacgttgc gttttgatta acgcaacgct
    24661 tcggcactct tacccctgct gctccagcgc atacttatac agcgcatttt tcttcacgcc
    24721 gtgaatttct gccgccagcg ccgccgcttt tttcaacggc agttctgcct gtagcagcgc
    24781 cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac cttcgataat
    24841 cagcaccatt tcgcctttgc gacggttttc atcttctttt acccacgcca gcagctcgcc
    24901 aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca gaaccacgta
    24961 gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac ggtgggtgga
    25021 ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat cacggcggcc
    25081 ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagagggta aacccgcggc
    25141 gcttaacgca gtgatagcag cacacggccc gggtagcggc accacgcaaa tccccgcttc
    25201 acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc cggcatcgga
    25261 aaccagcgca atgttctgcc cttcttgcag cttcgccagc agcgtttcgg ctttttgttg
    25321 ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt gcagcaataa
    25381 accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata cctctaacgc
    25441 acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa gctggccctg
    25501 agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga tttaatattg
    25561 agcattgcgt aaaaaaatat cactggatac attatggtac cctcaacatt ttctcgtttg
    25621 aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg ttgtggcacc
    25681 catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga ttctgccttt
    25741 tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca attactcgcc
    25801 attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt taaccaacta
    25861 ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggt agagattaaa
    25921 ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac acctgccgat
    25981 ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag ccaggggcgt
    26041 ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgctcgg cgcgaaagaa
    26101 aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca ggaacaggcg
    26161 aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga tctgcagcgc
    26221 gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc cgactggcaa
    26281 aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt taacgtaaaa
    26341 gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa tggccaggca
    26401 gcggtatttg gttgcactat tcagcaaggc tttgaagcgg cgaaaaatat cggcactcag
    26461 ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagctgaaca acctcagccg
    26521 caaaccgtgg atggcgttgc cagcccggca caagcctcgg ttagcgatct gaccggtgaa
    26581 cagcctgcgg cacagccagc gcctgtaagc accccggcag caaccaccac agcggtaagc
    26641 gcgccagcga atccatccgc agaactgaaa atctacgata cctcatcaca accacttagc
    26701 cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc gttgctgaaa
    26761 aataacgttg aagagttgct taagagcaac accccgctga acgtgctggc actgaaccag
    26821 ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc ggaagacgaa
    26881 gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct ggtgctgatc
    26941 ccacgcagtt cattgggcga tcgcgtagcc aatgcgtttg cgcaggagtg gcagaaactg
    27001 ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg cgcgggtgtt
    27061 aacggcggtt ctggtatcgc tttaacgggt accccgatta ctcccagagc gacaaccgac
    27121 tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga ccagttcacc
    27181 aataatggcg gtcgtgtcga tgcggtgtac attgtggcaa cgccgggtga aatcgctttt
    27241 atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct gtacgccagc
    27301 tcccgcagtg cgcaagggac cgctggcccg gatttccgac tggagatgga aggcttgcag
    27361 tacagcgaaa tcccgatgct ggcaggcggt aatctaccgt taatgcagca ggcactcagc
    27421 gcggtgaata acgattattc actggctcgc atgtatgcga tgggcgtcga tgcctggtcg
    27481 ctggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa cggtaatacc
    27541 ggaagcctga cggcaaaccc ggattgcgtg attaacagga agttatcatg gctacagtac
    27601 caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat gcgtgggaag
    27661 tacaagcgcg tcgctggctg gaaggcaaag gactgcgatt tgtcgccgct aacgtgaacg
    27721 agcgtggcgg cgagatcgat ctgataatgc gtgaaggccg gaccaccgtt tttgtcgagg
    27781 tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc cgcagcaaac
    27841 aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg agttttgata
    27901 ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag tggattaagg
    27961 atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga aagaattaaa
    28021 gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct tccggatgcc
    28081 atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa aatcctctgt
    28141 tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat gatcaaccgt
    28201 ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa tgttgtctta
    28261 acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt gcgggcgctg
    28321 ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg cgatattgta
    28381 aaagcggtag aagctgccgt tacgcgtgat atgactattg tggcattgac cggctatgac
    28441 ggcggcgaac ttgcaggttt gttagggcca caagatgtgg agatccgcat tccttcgcat
    28501 cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg cgatctgatc
    28561 gataacacgc ttttccctca ccaggatgtt taaggagaat acatgaaggc attatcgcca
    28621 atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc cgtagtgggt
    28681 accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac ccaggtggac
    28741 gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca gattaagaaa
    28801 gaagcccgta ttaatgtcac ggcgtatcag ggcaaagtgc tgctggttgg gcagtcacca
    28861 aacgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg tgccaacgaa
    28921 gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcgtc taacgatacg
    28981 tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa atcgtccaac
    29041 gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac tgaacgtgaa
    29101 gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt aaccacagcg
    29161 tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc cgcgtcttat
    29221 caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca tccgacatgt
    29281 tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga cactgccacc
    29341 atcgcccc
//
LOCUS       DAFATA010000050        28947 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFATA010000050 DAFATA010000000
VERSION     DAFATA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.051"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            386..1210
                     /locus_tag="LGJ20_002674"
     CDS             386..1210
                     /locus_tag="LGJ20_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBK2386715.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVEM
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVVLTRHHWSVMWSLNTHAR
                     LEIITGLFNRLQKTLRAMPPDDRDSLPLLYQTETFLKALNDTLARHELKQSSKVALPE
                     AIVKGYITRLENQPVQGEASSPVTLPAQALRSDAPDVQEHQALPHSRLVYVVSETKAE
                     PTPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSPEEQHFTWGNGCKKQ"
     gene            1452..4133
                     /locus_tag="LGJ20_002675"
     CDS             1452..4133
                     /locus_tag="LGJ20_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBK2386716.1"
                     /translation="MVTICVDGENKIHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAERVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKGSDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGGFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLSG"
     gene            4264..4440
                     /locus_tag="LGJ20_002676"
     CDS             4264..4440
                     /locus_tag="LGJ20_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2386717.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRVSVARIAREHDIND
                     NLLFKCKRTVKDVG"
     gene            complement(4444..5982)
                     /locus_tag="LGJ20_002677"
     CDS             complement(4444..5982)
                     /locus_tag="LGJ20_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK2386718.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFDSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARIIMAKFAEHLPLYRQSEIYARQGVELHRNTMGR
                     WVDIMGEQLRPLYDELKHYVLMPGKVHADDTPVNVLEPGQGKTRTGRLWVYVRDDRNA
                     GSTMPAAVWFSYSPDRKGIHPQQHLADYRSILQADAYAGYNALYESGQVTEAACMAHT
                     RRKIHDVHVRHPTTVTGEALRRIGALYAIESEIRGSPAEERLAVRKARTVPLMQSLYE
                     WLQGQMSTLSRHSDTAKAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRVVALGRRN
                     YMFFGSDGGGESAAVMYSLIGSCKLNGIEPETWLRHVISVINTWPANRVKELLPWNVT
                     LSVN"
     gene            complement(6032..6379)
                     /gene="tnpB"
                     /locus_tag="LGJ20_002678"
     CDS             complement(6032..6379)
                     /gene="tnpB"
                     /locus_tag="LGJ20_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK2386719.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGNVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(6376..6765)
                     /locus_tag="LGJ20_002679"
     CDS             complement(6376..6765)
                     /locus_tag="LGJ20_002679"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2386720.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPLLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLHFNGNVSEKLLTLLIQELKR"
     gene            complement(6929..8157)
                     /locus_tag="LGJ20_002680"
     CDS             complement(join(6929..7842,7842..8157))
                     /locus_tag="LGJ20_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBK2386721.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            complement(8353..8610)
                     /locus_tag="LGJ20_002681"
     CDS             complement(8353..8610)
                     /locus_tag="LGJ20_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386722.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTHKGT"
     gene            complement(8679..10376)
                     /locus_tag="LGJ20_002682"
     CDS             complement(8679..10376)
                     /locus_tag="LGJ20_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBK2386723.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            complement(10472..11608)
                     /locus_tag="LGJ20_002683"
     CDS             complement(10472..11608)
                     /locus_tag="LGJ20_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBK2386724.1"
                     /translation="MRKISMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            12284..12492
                     /locus_tag="LGJ20_002684"
                     /pseudo
     CDS             12284..12492
                     /locus_tag="LGJ20_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            12479..12791
                     /gene="tnpB"
                     /locus_tag="LGJ20_002685"
                     /pseudo
     CDS             12479..12791
                     /gene="tnpB"
                     /locus_tag="LGJ20_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            13758..14495
                     /locus_tag="LGJ20_002686"
     CDS             13758..14495
                     /locus_tag="LGJ20_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK2386725.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGLIESRVAIK"
     gene            15408..19499
                     /locus_tag="LGJ20_002687"
     CDS             15408..19499
                     /locus_tag="LGJ20_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2386726.1"
                     /translation="MNKIYALKYSCLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSMT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMIDFS
                     SVSRRGSLTLLSQGYGVSAKHGGLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHRFS
                     KLITEAAPADINISGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTVGT
                     PWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKWSLAGVTLHNNG
                     VNGQKNNWLLLPEDYIKNIITADFDPIISFNKNSKEHMSWTYDAAKGVGRIQQDDQQF
                     VMHGNLNGNLNAGKNLYFTGANGIIDLKDNVNQGAGYLQFADDYTVTTSNASSWFGGG
                     IIVNSGTTVKWGINGVSGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNND
                     STAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDINGKNTEFSRLQAVDYGAA
                     IINSSTDKSLLTLNLSPLKKDEIAVSVKALDMNAIFHGGYGTAGDLYKTTFYGPTQYY
                     LLKKPKFGSVLMGSLKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNM
                     DIVIPELTGNDILTLDGSVSISGNMSKQDGALILQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNNGTGNDVISVEGKS
                     AAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTGGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSS
                     VLKARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGVAVGLLSGFNTAYRGAI
                     HAPSASATVNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAY
                     AMRTDLKNADKLVVNKKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSE
                     QAIGFSNVTPVITAIDAGDKTTWNLTGYRMAENPATTQSASGLASVGYKSFLSEVNNL
                     NKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFT
                     VTHTDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGT
                     RDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDY
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSR
                     MLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            20362..20553
                     /locus_tag="LGJ20_002688"
     CDS             20362..20553
                     /locus_tag="LGJ20_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386727.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            20587..20910
                     /locus_tag="LGJ20_002689"
     CDS             20587..20910
                     /locus_tag="LGJ20_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HBK2386728.1"
                     /translation="MAYHSPDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            21329..21589
                     /locus_tag="LGJ20_002690"
                     /pseudo
     CDS             21329..21589
                     /locus_tag="LGJ20_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21599..22750)
                     /locus_tag="LGJ20_002691"
     CDS             complement(21599..22750)
                     /locus_tag="LGJ20_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HBK2386729.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            22812..23708
                     /locus_tag="LGJ20_002692"
                     /pseudo
     CDS             22812..23708
                     /locus_tag="LGJ20_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            23759..24202
                     /locus_tag="LGJ20_002693"
                     /pseudo
     CDS             23759..24202
                     /locus_tag="LGJ20_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
     gene            24364..25866
                     /locus_tag="LGJ20_002694"
     CDS             24364..25866
                     /locus_tag="LGJ20_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBK2386730.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPIPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMMGSHPNPCDRLHSWPNNYQEQFPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(26225..27268)
                     /locus_tag="LGJ20_002695"
     CDS             complement(26225..27268)
                     /locus_tag="LGJ20_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK2386731.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLRRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            complement(27524..28789)
                     /locus_tag="LGJ20_002696"
     CDS             complement(27524..28789)
                     /locus_tag="LGJ20_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBK2386732.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
BASE COUNT         7688 a         6556 c         6716 g         7987 t
ORIGIN      
        1 ttattatcag gtttatttaa ttcaccatga cccgttattt cacgatattc agatgcgtct
       61 tttttatctt aatcatttcc cgtattccta gcactggaat gtcagattca gaccccagga
      121 agcggtctat attagcaacg atacctgtca tatcaccatt acctgtaatc agtccggttt
      181 tcatttaacc agggatggac aacgactgtt cactgaacgt tatatcagaa accttaatga
      241 gctgttgtcg gttctgaaac ggcggtggga tgtgacaccg gcaatcatcc gtgccgtgga
      301 atacctttcc cggggcccgg tattacactg acattctttt cgtcactgtt aatcatgctt
      361 taatacagta acactgaatc tgccgatgac ttctgaagtg aaactgaaaa ccggtggtga
      421 ccccaggtca ttaccggatt atgctgctct gcgtgatgaa atcagcaaac tgacccaccc
      481 ggcacgtccg gatgtggact ggcgttatgt ggagatgctc tgcctgcgtc tgtatgaaca
      541 caatggcgtg gaattacaga cggcatcctg gtacaccatt gcccggatgc atacaacagg
      601 gttaagtggc ctgaatgaag ggctggctct gattgtggtg ctgacccggc atcactggtc
      661 agtgatgtgg tcgctgaata cacacgcccg gctggaaatt attaccggtc tgttcaaccg
      721 tctgcaaaaa acgctccggg ccatgccacc ggacgaccgt gacagcctgc ccctgctgta
      781 tcagaccgaa acattcctga aggcactgaa tgacacacta gcccggcacg aacttaaaca
      841 gagcagtaaa gtggctttac cggaagccat agtgaaggga tatatcaccc gtctggaaaa
      901 tcagccggtg cagggcgaag cgtcgtctcc ggtaaccctt ccggctcagg cacttcggtc
      961 tgatgctccg gatgttcagg aacatcaggc cctgccgcac tcccgtctgg tgtatgttgt
     1021 ttctgaaaca aaagcagagc ccacaccctc ccttcaaaaa tcccctccca cgtttctgaa
     1081 accgtttgtg gctggtgtct gtgcggcatt gctggcggtc agcgtggcta ttccgggatg
     1141 gcagtttctg acacagccat caccggagga gcagcatttt acctggggga atggttgtaa
     1201 aaagcagtga aaacagtaaa tacagagtat cgtgatgaat tagtgctcag ggggctacca
     1261 gtattcaata accagcgtat cggcatcgca ttcctgtaat tctggtattg actgttatga
     1321 ctaaactcaa tgtatatatg gcaggcagaa atatgttaac agtcccggag ttgtggaaaa
     1381 tcctctggag ctaatatcgg gagtgcctta acaattttcc gtaagttaaa tgataattcc
     1441 tgagtgagaa tatggtcacg atttgtgttg acggtgaaaa taagattcat aaaattactg
     1501 actggacgct gtgggctgga aatgacgaca gagaggttat gctgacctgt cattttcgca
     1561 gcgggagaaa atatactcgt ccgctttctg tatgtcagat aacgccaact gtaaatttgc
     1621 gaaatgtttt cctcgaaagg aaagggaacg ctgtaacatc ccgggctgaa cgggttatta
     1681 tatatggtga taaatatgct gctgtttatt atcgcgaggg tgaacgaccc tacatcatga
     1741 aaacgaccgg ccttgacttt caacagtgtt cagcatttac agaacatgcc gtgtttaatt
     1801 acttgtgtag ggtggctaat gaacgtattt tttatgcccg cggtaataac agaaatatcg
     1861 atgagaatat ccttaggcag ataaagaaaa tagttcctca tccggatacc gccttgcatg
     1921 catactgttc aggacagagt aaaaaacgtg attcgccatg gggtttgatt tttccgtttg
     1981 gtcttaatga aagtcagttg ctggctgtcg aacgggcatt ttcctcacag gtttcagtta
     2041 ttgaaggacc acctggaaca ggtaaaacac agactatact caatattgtt gctaatatat
     2101 taattcagaa taaaacagtg gcaattttgt ccaataataa ctcagctgtc agtaatgttt
     2161 atgaaaagat ggacaaacag caactgggat atgtggtggc acgactgggg agtacggaga
     2221 acaggcaaca gtttttttca acgtcgattt cgcgttctga ggaagtctta cctgactcac
     2281 cgtctgcaaa tgcgattgat gatgtacttc aacaagtgaa aaaacacctg aatgccatta
     2341 accaggtcgc ttcgttaaaa gcagaaatta atgaactgaa tattgagtat aaatacctgc
     2401 agcaatggca gagtcagaat cttcgaccgg aggagctttt ttctcacaaa taccgacttt
     2461 cctcacgaaa aacaactgac ctgatggctt atattcatta cctttctgac aggcggattg
     2521 gattcagaaa cagaattgat ttgttgttaa acttcaggat actgaaagtc aaaccattgg
     2581 tgattccgga gagaaggctg gcactattta cttctcttca gctttcttat tatgaaaaat
     2641 tcatccggga aaaacaagtt tctctgaatg aatatgagga agtattaaag ggatctgatt
     2701 ttaaaatttt actggagcgc ctgacatcat ggtctgtgct ttatttaaaa caacatcttc
     2761 gaagaaatat ttcaacaaga aattcattct cggcagagac atacagggat gaatttaacc
     2821 gttttataaa gcgttttccg gttattggta gcagcacgca ttctattatt aactctatcg
     2881 gtaagggggc tcttcttgat tatgtgatta ttgatgaagc ctcacagcag gatattgttc
     2941 cgggcattct gggactggga tgtgcccgta atgtaatcgt tgtaggggat cgaaagcaac
     3001 ttccacatgt tcctgtatta ctcccgaaca gcccatctcc accggcagaa tactataatt
     3061 gtgagaaata tagtctactt gattcggttt gtatgttgtt caggaatatg gtgccagtaa
     3121 ctcttcttaa agagcattat cgctgtcatc ctaaaatcat tcaattttgt aataagcagt
     3181 tttatgataa tgctctgata ccgctaacgc ttgattcagg tgaagcctca ttatctctga
     3241 tcatcacggc aaaaggaaac catacccgta atttttcaaa tctcagggag cttgaatctc
     3301 tggaaggaca ttactgggat gaggaaagta gcaggggata tatcgcccca tataatgcac
     3361 aggttaatct ggctgaaaca gtgctgcctg ctgattttgt gaaatcgacg gtgcataaat
     3421 ttcagggacg ggagtgcgat gaaattgtgt tttccactgt gctggataag aagcgcagca
     3481 gccagcacag tcggaacatc gcgtttgttg ataatcctga actggtgaat gtcgctgttt
     3541 caagggcccg aaataaattt acgctggtga ctggtaatga tgtatttgaa cgccacgctg
     3601 gacatattgc ggcgctgatt cgttacatca aatactacgc tgatgacgga gagattttcg
     3661 agagcccggt catatcagca tttgatttgc tgtacagtga gtatgacaaa tcactggaac
     3721 gactgaatag tagattgaat tcaaatgatt cacactttaa gtcagaacag attgtggcct
     3781 gcctgttgcg ggatatcctg tcgcaggata gttatcggag tatgatgttt cattcacaaa
     3841 tagcgctgaa tcagttggtg ttgctggagc gggggggttt tacacaccgt gaacagttat
     3901 tcatgcgtaa ccgggccagt tgtgactttg ttgtttacta taaggtcggg aagactccat
     3961 taggggttat tgaagtggat ggaggttatc atctgaccag tgttcaggct gaacgtgatg
     4021 aacttaaaaa cagtatactg aagaaatgtg gtctgccatt gctaagatta cgtactatag
     4081 acagtgatat agaagggaag ttgggcgcgt ttttgtcagg cctgtcaggc taaactctga
     4141 atattaaaaa ttgcatatag cactattagt tttctggtaa gcgtaaaccg accgtcgtat
     4201 gtagccatta agccagtatt ggtaacgtag gtgcccacct tttcaaacta gtggacgcct
     4261 gttatggaac aaaaaacatt atccgcagaa ccccgcaggt cattttcaaa tgagtttaaa
     4321 cttcaaatgg ttaaactggc tttacaacca agggtttctg ttgcccgtat tgcccgggag
     4381 catgatatca atgataatct tctgttcaaa tgtaagcgca ccgtgaagga cgtggggtaa
     4441 aaattagttt acagatagag tgacgttcca gggcaacaac tctttcacgc ggttggctgg
     4501 ccaggtgttg atgacactga tcacgtggcg tagccacgtt tccggctcga ttccgttaag
     4561 tttgcagcta ccgatcaggc tgtacatcac tgccgcactc tcgcctcctc catcagagcc
     4621 aaagaacatg tagttacgcc gacccagtgc aactacccgg agggcgtttt cacacaggtt
     4681 attgtcgatc tccacccagc cattgcggca gtattcgttc agggcgtccc attgcttcag
     4741 cagataggtg aacgctttcg ccgtatccga gtgacgcgac agcgtgctca tctgcccctg
     4801 gagccactca tacaacgact gcattagcgg taccgttctg gcttttctga ccgccagtcg
     4861 ctcttctgcc ggactgccgc ggatctcaga ctcgatagcg tacagtgccc cgatacgacg
     4921 gagcgcttct cccgttactg tcgttggatg gcggacatgt acatcgtgga tcttgcgtcg
     4981 ggtatgtgcc atacaagccg cttcggttac ctgaccgctt tcgtaaagag cattgtaacc
     5041 cgcatatgca tcggcctgca ggatacttct gtagtccgcc agatgttgct gcgggtggat
     5101 acctttgcgg tcgggagagt atgagaacca caccgctgcc ggcatggtcg aaccggcgtt
     5161 gcgatcgtca cgaacataaa cccacagccg tccggtacgg gttttaccct gacccggctc
     5221 cagtacattt accggcgtgt catcggcatg cactttaccc ggcatcagca catagtgctt
     5281 cagttcatca tacagcgggc gaagctgctc tcccatgatg tcaacccagc gccccatcgt
     5341 attgcggtgc agctccacgc cctggcgggc atagatttcc gactgacggt acagcggcag
     5401 atgctcggcg aacttagcca tgattatgcg ggccagcagc cccggtccgg cgataccccg
     5461 ctcgatgggc cgcgaaggtg caggtgcctg cacgatggca tcgcactgag tacaggcatg
     5521 tttttcccgt accgtccgga taacccggaa ggcgctacgc atcaactcca gctgttcggc
     5581 ggtatcctcg cccagatagc tcagtgaacc gccgcagttc gggcagcacg gcgccgcagg
     5641 caacagtcgc ttttcgtcac ggggtagtga ttcagggaac ggcttacggg tgcgggtctg
     5701 acgcaacgga cgctgtacag ccgggtcata caccctacca gtcagcgtat cgctctcttt
     5761 ctgaagccgg ttcagatcgg cttccatttg tgcgatacgg cgggagactt tttcggaacg
     5821 actgtcgaag ttcatccggc ggagtttatc cagctgcgcc tgcagatggt ctatttcgcg
     5881 ctcccggttg ctcagctttt cctgcagggc gtggatcagc gcttcctgtt cggccaggcg
     5941 ctgtttcagc aggaagatgt cgtcagaaga gatgtcgttc ataagcccgt attttaccgg
     6001 gcttattctg tgacaaccag gataaagaga tttacagcat ggtcagggag gtcagcaacc
     6061 gcttgggctg tcgccagtcg ataccttcca gcagcatcgc cagctgcgcc tgcgtaagga
     6121 acacgttgcc atcacgggct gacggccagg cgaagcgccc acgctccagc cgtttggtca
     6181 ggaggcacag tccgtcaccg gtggaccaca gcagtttaac ctgactgccg ctgcggcccc
     6241 ggaaaatgaa aacatggccg gacatgggat cgtctttcag cgccgtttgt actttcgcag
     6301 ccaggccgtt gaagccattt ctcatatcgg tgataccggc aaccagccaa attttggtcc
     6361 cggaaggtaa cgggatcatc gcttcagttc ctgtatcagc agagtcagga gcttttcgct
     6421 gacattgcca ttgaagtgga gcgtcccgtg ccggaacgtt acctcacagc tgatactgag
     6481 ggtttccggg tcctctgcga gcgattctgg ctgttcggca gctgcatcga gagtcacagg
     6541 aagtagcagg gggctctctg aagaaggtaa tagcagcttt ccctcgcgcc attgttgtcg
     6601 ccatttgaac aacagattgg cgttaatgcc attttcaaga gcaagttttg agatggatat
     6661 cccgggttca caggaggcag caacgagctg ctgtttaaat tcgggaggat aattagggca
     6721 gccttttcgc ctgccgggag tcacattttt ctgcatatct gacactttgg ttcccactac
     6781 ttatttggtg gacaccactt tgtctaattc gtcagattct gaccagacgg ttcaggctgt
     6841 acgcttacgg ttgtcgtgaa ggagggggaa gaccatctca tcgcttactg atgttcctgt
     6901 tactatatct attgaattgg atttgcccct atatttccag acatctgtta tcacttaacc
     6961 cattacaagc ccgctgccgc agatattccc gtggcgagcg ataacccagc gcactatgcg
     7021 gatgccattc gttataatgc tcgaacgcct ctgcaaggtt ctttgctgcc gttaacccgt
     7081 ctggtttggg catgatactg atgtagtcac gctttatcgt tttcacgaag ctctctgcta
     7141 ttccgttact ctccggactc cgcaccgccg tgttcttcgg ttcaagtccc aacatccggg
     7201 cgaactggcg tgtttcatta gcccggtagc atgaaccatt atccgtcagc cactccactg
     7261 gagacgatgg aagatcgttg ccgaagcggc gttccaccgc tcccagcatg acgtcctgta
     7321 ctgtttcact gttgaagccg ccggtagtca ccgcccagtg cagtgcctca cgatcacagc
     7381 agtccagcgc gaacgtgaca cgcagtctct ctccgttatc acagcagaac tcgaacccgt
     7441 cagagcacca tcgctgattg ctttctttca cggccactct gcctgtatgt gcccgtttcg
     7501 atggcggtac agcaggtttt cgctcaagca acagcgcatt ctggcgcatg atccggtaaa
     7561 cacgtttggc attgatcgca ggcataccat caagttctgc ctgtctgcga agcagcgccc
     7621 atacccgacg ataaccatac gtgggcagct ccccgataac atggtgtata cggagaagca
     7681 catccgtatc atcagtgtga cgactgcggc ggccatccat ccagtcatcg gttcgtctga
     7741 gaatgacgtg caactgcgca cgcgacaccc ggagacaacg gctgactaag cttactcccc
     7801 atccccgggc aataagggcg cgtgcgctat ccactttttt gcccgtccat attcaacggc
     7861 ttctttgagg agttcatttt ccatcgtttt cttgccgagc aggcgctgga gttctttaat
     7921 ctgcttcatg gcggcagcaa gctcagaggc aggaacaacc tgttctccgg cggccacagc
     7981 agtaagactt ccttcctggt attgcttacg ccagagaaat aactggctgg ctgctacacc
     8041 atgttgccgg gcaacgaggg agaccgtcat ccccggttca aagctctgct gaacaattgc
     8101 gatcttttcc tgtgtggtac gccgtctgcg tttctccggc cctaagacat caatcatctg
     8161 ttctccaatg actagtctaa aaactagtat taagactatc acttaaataa gtgatactgg
     8221 ttgtctggag attcaggggg ccagtctatg aatcctggct ataagttcgc tgtttaaaga
     8281 attatatgaa tagcaccaat taataagtta atatgaaaaa gaagacagac cttctaaata
     8341 aacgcattga atctatgttc ccttgtgagt gtttttacgt ttggcgggag gcctggaacc
     8401 tcgggattta acttttcttt ttatattttt gataaaatca tcaatagaaa actgaatcgt
     8461 ctctgataaa tactgttgag ctatatctgt actctgtttt tctaaatcag aagctttctt
     8521 tgtttcaaga acagatttga ttaactcctt acgttgtgta caccaaatct cgtcagatga
     8581 acagtatttt ttattttcgt gtttgttcat tatttattgc tctaactgat tgatttccct
     8641 atatccaggg agtagatata tctactccct gcttattatc atttcacgtt agttaaagat
     8701 ttttttatat tctcaaattt ggcttggttg atatcactga tcggtttttg acttgacgtg
     8761 atgaagtcaa tcataacagc cttcatattc ttaactacat caggatggga tgcagagaga
     8821 tctttagttt gttgcagatc gttgatatcg tacagagcta gtttattctc tgcataggtg
     8881 taaaccagag aataatcgtt gcttcttaca gtgtatgcaa actggcttaa gtcttctgta
     8941 tgtgggttgt gcggataatc atcagactct tttctcacga atttatgata tccgttccag
     9001 aatgcagtgt tctcttcgtc ccaccagtga gaataagagg taatccagga cagatatttg
     9061 tgaggttcgc cagctgcaga tttcttatca gctcctttct gtacatatgg caataatgat
     9121 acaccatcta gtttcagaga agcagggatc ttgatatctg cggcatctaa agcggtcgga
     9181 tagaagtcca ttgatgaaac cagtttgtca taatcacccg ttttcagctt gccattccac
     9241 cacatgaaca taggtgtatg cataccaccc ggatacggtt ggcctttgta accagcctgc
     9301 gcaccattca gtggcagtgg accatcaatt acagcaccgt tatcagaggt gaacagaatg
     9361 atggtgttgt cgtactgacc tttttcctta agtgtattca ggatcttttt aaccccctgg
     9421 tcaacggagt aaattgacgc ataatagtta tcagcagtct tgctaccagt gttgaatttc
     9481 tcctgatagt ttttaggcgc aggctgatcg ttaggcaggt gtggtgcact gtatgccaga
     9541 taaatcataa atggctgttc cagtttgctt gcacggtcaa ttacaccaat agcttcatca
     9601 gttaattgat cactaatgta cccttcagct ttaatgtttt cacggttacg gaacaatgac
     9661 ggggagttat aaagtgaaac gccggatgcg tgatagccca tgaagtagtt gaagccacga
     9721 ttctgaggtt gccattcttc tgcggagaag gtggtgaagt tatcgtggta gtcacgggtt
     9781 tgtttttctt caggcacacc tacgttgctg atttttgata agtgccactt accgattgct
     9841 gcagtgtaat aaccatgatt ctgaagtaat tcagacagga atgtttcttc cagagggata
     9901 ccatcctgtg catcagaatt tgaatataca ccgaagcgag caggagctcg cgccgtcagg
     9961 attgcggcac gtgaagggcc agagacaccg tgtgcaacat agccgttagt gaattttaca
    10021 ccttcgtcca tcagggccgc taatgttgga gtggattttt ttgcagcttc aattgcttta
    10081 tcaataccta ttttgtatgt atcaacaaca tttcggttat ccatcgtttt agggtcaaat
    10141 gaactatcat caaaaggcaa ttgtccatac cctaaatcat ccattgttaa aacaatgata
    10201 tttggcttac ctttagtgct gtattccgtc ggcgtgaagt ccgcaaaggc aacattggtt
    10261 tttgttgctt tgagcttaac atcatctgct gcatttgcag tactcatcat cagaccagat
    10321 gctaatataa gtgatattga tgagttaagc agttttttat ttaactgatg ttccatatgg
    10381 tattccttct gagttgcatc acgaacattt attaataata aaagcctctg atataaataa
    10441 aaactatgta aatcgtttaa atggatacct gttaaaactg atacatcata ccaacagcaa
    10501 gtatattatc ggttgcaact ccagcctttt tggtaaattc gctttcatca ataaggttaa
    10561 ttttgtaatc aataagaagt gacatgtttt tgttcagaaa gtagtttgcg ccaacatcaa
    10621 tatactcaac aagagactga tcgccatagt ttactattcc acctttaaca tccatccctt
    10681 tggactgaac gtaagccagt gaaggacgca ggccgaaatc aaacatatac tgagccacaa
    10741 cttcaatatt ccgtgcttta tctgcgatgc ctgcgctacc aaatggagtc atatttcggg
    10801 tttcagagta gtcagctgcc agatacaggt cattatcatt atacttgacc ccaacacctg
    10861 caaactccgc atgttttccc ccggcaatcc agcgaggcgt aataacttct tttccttctg
    10921 aatctttcga tgtcatgaaa cgtttacggc cttctttttc ctgcagactg gttctgtcgg
    10981 cattagtgta agcggcaacg attccaaaac cttcatagtc atagcttacg gagaaaccat
    11041 atccatcacc attagcttca agtaaattat cacggtcatt tttaccctga tattgagcag
    11101 aaaaagtaag tccatcaaca agcccgaaga aatctgtatt gcgccaggtc agaactccgg
    11161 ttgttctgcc ggtcatatag ttatcagagt aagtccagct gtcaccacca aagtccggaa
    11221 gtacgtcagt cagagccccc acatcatagg caacaccata gtttcgccca taatcaagtg
    11281 aaccgagtat attgtggcga atacctgcaa atgccagacg atttttatcg ttgcgtactt
    11341 cactttctgt cttaaacccc aggaactggt attcccactg accaaagcca gtcaggctct
    11401 cattaatttg tgtttcgcct ttgaaaccaa gacgtgcaaa tgttctgtcg ccatccatag
    11461 aaccatcaga gatgtaatga cgggctttaa cgcgaccata aaaatccagt ttattaccgt
    11521 ctttattata gatttctgct gcctgtgttg cctcacacag cattaatgca gaaacgacag
    11581 agcacacaac catacttatt tttcgcataa aaaaatcccc tgtgcacatt tttagtatgg
    11641 ttttaaaaat taaactatgg cgaaaaaatt atcactatgc aaaaatgtaa caaaatattg
    11701 ccaggtgatc tatattgttg tttttatttg aggttgtata ttttctagtg tatttgactt
    11761 ttctttctca agtgttattt cttgaccttt atcacagaac catctccctg ggtatcccca
    11821 tcacattgca tgaaaagcaa gttttaccaa tcttgatttt cagtgttaaa taagattaat
    11881 aactgtaatt ctgcaaaaaa aagaacacca tatgtagtat gactacgtat tccactctgt
    11941 tcgttcacgg actggcatcg aagtctgagg tgtactggca atagcggata cagtcatttg
    12001 ttcttttttt aagcattcat ccgatgatac ttttcctgaa tgctaccgga gtggtattcc
    12061 cctgccgtga gggagggcgc tgacggttat aaaaatcctg atatataccc attcccgtat
    12121 gacagcgatg gcgtcacatc tctgttctac catcttcaca gaaatatttc gttcattagc
    12181 attccggtat tcgtataacc accttcggtg gagaaagaat aaacaatccc tggaatgcga
    12241 tgagcgcttg accagaaaag catcatattg cttcatggcg catcagcgac cacaggatgc
    12301 cgcaaaagcc gaaaaacgga ccagagcgcc tgaaatcatt cctcgcctca gtatctgttg
    12361 cccatggagt ggccgataac agatcattgt cggatgagcc ttatcccctt ccttcccggt
    12421 ggtattcgga tgtgctgcca gccttctcag ttaaccgatg ttttccgggc tttttgccat
    12481 actgaggact gataacgtat tcctggtcgt aaaacccgtc aatatgcgcc ggggcatcga
    12541 caaaactgac acagtacgtc cggaaagaac ttaacgctgc atggcacgac aatgcagcct
    12601 ttgtctttac caacaaggcc cgttcccgca taaaagttct acgccgggat aaacatggcg
    12661 tatggctgcg tcaggccttc actacttcac accggaagtc ttccaccctg acacgaatat
    12721 accgtccggg ctctttcgca ggtgcaaaca gtttttcaca ttcggcgaat gtcatttctg
    12781 aatcctggta acgcaaccac tgaaccggca tattaccggg tttgattatt gtcgcagtta
    12841 ttttatatcg tttccccata tcgcctctca tcctgacaac aaaattaccg gctgagccat
    12901 cactccggta aagtatgcga aaaaaagtga gcgtctcagc aatggctcat gcgatgattc
    12961 tgtacgggca ccgatggcac ctcagaatac aacaaagata tcagagtaac atgtctctct
    13021 cgttattttt cagagttctt gacaccctcc ttctgccaca ttgtcaacgc cagtggagac
    13081 ttctgctgtc agttttttca tccatacaga gtgacattaa ttatcctgaa caaaacgaaa
    13141 tatcctgaat atatttattt tctcctggtg ccggagacat gatgatgtac aggctcaggt
    13201 gcggtgtgaa tgtaaaatgg tatgttaatt attcatttct gaatgcaaac tgaatgcaaa
    13261 cttattccga attgaattct cctgacacca catgataaaa atgaggatat aataaaatac
    13321 ttctcttctg gatgtgccat tacctgccgt tgctgtaata cggcgggtat tttttactga
    13381 catatcggga agccagcaga cgttcgtagc tacatccccg aaagtggcac actgcaaact
    13441 ataatacgag taaaacatcg atcaccgtag ctgatgtgtt caacgatcgt gcactagcgt
    13501 tctgcgattc tcagggactg tctgttctga gaatcggcga gtccgcgatc ggatacgatg
    13561 cgatttcgca gtgagtcagg gattgcatct gaaaaccctc cggctatgtc ggtaaatatt
    13621 tatttctatg aaccgtacga ttcagatagc attcgcccgg ccccgtaaga ataatctccg
    13681 gacaaataag atttttcctg aaaaagcgtc tagtcttttg atatcgttgt ttttatgcta
    13741 atcagggagt atgtgtgatg attgtctgtt acgttattct tgttaaccgt ccgtttgaaa
    13801 cagagattgc cgggaaaaag atattttttc agaaaaatga cgtcctgttg acagataaca
    13861 tcagccagtc ctttttcctg cgccatcccg gtaatgttac tgagctccgg attgatgaat
    13921 gtattgtcag aaaatatatg gaaagtgccg acaggatcca gcactctgat agtgctgttt
    13981 ctgttccgtt cattaaaaca accttctctc acatcgctct gttacataat gtcatgcaga
    14041 atctcagtct gcaaaagaca ttgccacaac aattttctga gcagatgttt tttgcctgcc
    14101 tttccatttt ttccacacac agaggtttca tgtttctgct attcagatgc ataaactctg
    14161 ttgcgcttaa agtcaggtat attatctgtt ccagtccatc acgggcatgg agagttaaag
    14221 atatcagtaa ttctctgtac atgagcgaga gtttactgaa aagaaaactt caggaggaaa
    14281 aagtttcttt ctcccaaatc ctgctggata tacgcatgca taacgcccga caacttattt
    14341 ccagtaaact gtcagtgaat caaatcgcaa tacggtgcgg ttatgccagc acaccgtatt
    14401 ttatcagcgt tttcagagaa tattttggca taacacctca tcagatgatg attaatcagt
    14461 ccggacttat tgagagcaga gtcgcaatta agtaaagtat gtcagtcagc gcataataaa
    14521 agtattctta tgcttcatta tcagtttctg tttatcactc cggagcactg tagtaactac
    14581 ggccccggaa aacagatggt aatgagttga ctgactgatg atattgtggc gacattttta
    14641 cggatgcgag caaagaatgt attggtccgt ttgattatgc agtgattaac aaaactccgg
    14701 gaagatgaat gagtatacct ctcctgcaat ccgtttcctg agaaaacagg tggtaacaca
    14761 atattcttaa tatgttactg tttcaccaca caaccaggtt tacggttgtg tggtgaacta
    14821 tagtaatccc gggtaacaat ccggagctgt tccttttata ctcttcaggc tgtgtactca
    14881 tccacgccat ccgttttaca gtctcagtgt gtgaataata aattacactt tgaagtgaac
    14941 gccagtcagg accatgtcgt atttccggat ggatgagcat atatcagaaa ctacaattac
    15001 tacagataac gcttttcagt aattctgtca taacttgccg gaatgtaatg ttttgtgaca
    15061 tggtccttat tttttataca caggcaggtt ttgtcgggat ggaatggata atgaactctt
    15121 atcattataa ggcggcaacc tcttttttat gtgattaact ttcattatta ccaattctgt
    15181 ttatcgattc agctctggaa atacagaaaa attcagtgca ggtgtataaa tcatcaccat
    15241 gcgaaataca taaaatatat taatgtttgt tttccacagg aaaaatctta agtatggaca
    15301 ggtattcctg atgccattta aaatagattc agggtgagtg ttcagcactc accctttccg
    15361 gacggataat atattttaat actatataat tatataatag gaattatatg aataaaatat
    15421 acgcgcttaa atatagctgc cttactggtg ggcttatagc tgtgtcagaa ttaagtaaga
    15481 aagttacggg aaaaaccgga agaagattaa tgacggtttc cctggtatta tcaatgactc
    15541 tttctgcttt accgggtaaa gcatcaacgg tcagcgcaga aataccatat cagacttttc
    15601 gtgactttgc tgaaaataaa ggtgtgttta cccccggagt gacaggaatt gaaataaaag
    15661 ataataatgg aaatgcagtt ggtacgcttg atgtgccaat gattgacttc agcagtgtgt
    15721 ctcggcgggg ctcactgacc ctgttgtcgc agggatatgg tgtatcagca aaacacggag
    15781 gccttggtga tgtaaacaat gccagttttg gctatgataa aaataattat actgtagtaa
    15841 agaacaacaa acattcgggc cttgattttt ctctgcatcg gttcagcaaa ctcatcacag
    15901 aggctgcacc tgctgatata aatatatccg gacaacttag tgattcgtca cagtatactg
    15961 ctttttacag agcaggcgct ggcacacagt atattaaaga acgctcaggt aaacagactc
    16021 atatcccggg aacattttta actggtggaa cagtaggcac cccatggtat tcaggaaata
    16081 atttaatatc aagctctcca ggtgacacct ataataaatc acagggacca ttagccagtt
    16141 atgggcagat gggtgacagc gggtcgccat tatttgctta tgattctctc agtgaaaaat
    16201 ggtctctggc tggagtcacc ctgcataata atggtgttaa tggccaaaaa aacaactggt
    16261 tgttattacc tgaagattat attaaaaata ttattacagc cgatttcgat cccattattt
    16321 catttaataa aaacagtaaa gaacatatgt catggacata tgatgcagcc aaaggcgtgg
    16381 gccgtattca gcaggatgac caacagtttg tgatgcatgg taacctgaat ggtaatctga
    16441 atgcaggtaa aaatctttat tttacaggcg caaatggcat tattgattta aaagataatg
    16501 ttaatcaggg agccggatat cttcagtttg cggatgatta tacagtaaca acgagcaatg
    16561 ccagttcatg gttcggagga ggcattattg tcaattcggg tacaactgta aaatggggta
    16621 taaacggggt ttccggagat gaccttcata aagttgggga cggaacgctt attattaatg
    16681 gcacagggaa aaatgaaggg ggactgaaga ttggggctgg tactgtaatc cttgaacaaa
    16741 aagcgaaaaa caacgattca acggcttttt cttccattaa catcagtggg ggaaactcgc
    16801 gcgttaaact ttcaggagat aatcagatta tacctgataa tgtttcctgg ggattccggg
    16861 gtggatattt agatataaac ggaaaaaaca cagagttctc ccgcttacag gctgttgatt
    16921 atggggctgc aattattaac agcagcacag acaagtcatt gttgacgtta aatctgtcac
    16981 cattgaaaaa agatgaaata gctgtgagcg tgaaggcgtt agatatgaac gccatctttc
    17041 atggaggata cgggacagcc ggagatttgt ataaaacaac tttttacggt ccgacacagt
    17101 attatctgct gaaaaaacct aaatttggca gtgtgctgat ggggtcactg aaaaacacca
    17161 gcgaatggca atttgcagga acagacctga atcaggccgt tgatatggca aagaataata
    17221 aactgacatc aagtgcacag gcttcatatc tttatcacgg taagctcctg gggaatatgg
    17281 atattgtcat tccggagctt acaggcaatg atattttaac gcttgatggc tctgtcagta
    17341 tatcaggaaa tatgtcaaaa caggacggtg ctcttatctt acagggacac ccggttattc
    17401 atgcagggca aactgtttct gcatcgcaga gtgactggga gaacagggag ttctcactca
    17461 acaatctgaa tcttaataat gcggacttca gtctgtcccg taatgcattt atgaacggga
    17521 atatcagggc cgttaaccag agcactgtta ttatcggcgg agatacagtc tttactgata
    17581 aaaataacgg aacaggtaat gatgtcatca gtgttgaagg gaaatctgct gccgcaggaa
    17641 catcctccta tacagggcat atcactctgg agcaaaaatc agcactggat atccgcgata
    17701 attttcgtgg cggggttacg tctgaagaca gtcatatcaa tgtttcttca tcttcagtcc
    17761 tgttctcaga tgcatcgtca tttataaaca gctccctgaa tattcataaa ggaggagcgc
    17821 tgaccgctca gggagggctg tttacaggtg gaagcattga tatcggtgac gcttcccttc
    17881 tgcttaccgg tacacctgtg aattcagatg atgctgcttt tttaccgacc atcaatatgg
    17941 ctgatggcgg atttaaactg atgtctgatt catcagtact gaaagccaga gaccaggcat
    18001 ctgttgttgg tgatattatt tctgataaac aggccacaat cagcttcgga actgaatcag
    18061 gtaaagaggg catattgtct gagaaggcat cccggggagt tgcggtcgga ttactgagtg
    18121 gttttaatac ggcataccgc ggtgcaattc atgccccgtc tgcatctgcc actgtgaaca
    18181 atacctggtg gcaactgaca ggagactcct cacttcgttc gttaaaaaat accggaagca
    18241 tgacatattt tacaggaagt gcagcgaata aagcattcca tacactgacg gttgatgagc
    18301 tgacgacgaa tggcactgcg tatgccatgc gtacggacct gaaaaatgcg gataagctgg
    18361 tagtaaataa aaagctgtca ggtaaggaca atattctgct ggttgatttt ctgaacaaac
    18421 ccaccggaga aaaactggat attgaactgg tgagtgcacc ggggaacagc agtaaggatg
    18481 tttttaaggg aagtgaacag gcaataggct tcagcaatgt cacacctgtt attacagcta
    18541 tagacgccgg agataaaaca acatggaacc tgaccgggta caggatggca gaaaatcccg
    18601 ccacaaccca aagtgcctca ggccttgcat ctgtggggta caaatcattt ttgagtgagg
    18661 tcaacaacct gaataaacgt atgggtgacc tgcgtgacat caatggtgaa gctggcgcat
    18721 gggcacgtat catgagcgga accggctctg ccggtggtgg tttcagtgac aaccacacac
    18781 atgttcaggt cggtgtcgac aaaaaacatg agctggacgg actggatttg tttaccggct
    18841 tcactgtcac acacactgac agcagtgcct ctgctgatgc tttcaaaggt aaaacgaagt
    18901 ctgtgggggc cggactctat gcttctgcca tgtttgattc cggtgcctat atcgacctga
    18961 ttggtaagta tgttcatcat gataatgagt acaccgcaac ctttgccgga ctcggaaccc
    19021 gtgattacag tacgcattca tggtatgccg gtgctgaagc aggctaccgc tgtcatgtca
    19081 ctgaggatac ctggattgag ccacaggcag aactggttta cggtgctgta tccggtaaac
    19141 agtttgcatg gaaggaccag gggatgcatc tgtccatgaa ggacagggac tacaatccgc
    19201 tgattggtcg taccggtgtg gatgtgggta aatccttctc aggtaaggac tggaaagtga
    19261 cagcccgtgc cggtctgggc taccagttcg acctgctggc taacggcgag acagtattgc
    19321 gggacgcatc aggtgaaaaa cgtatcaaag gtgaaaagga cagccgtatg ctgatgtccg
    19381 ttggcctgaa tgcagaaatc agggataacg tccgctttgg actggagttt gagaaatccg
    19441 cctttggtaa gtacaacgtt gataatgctg tcaacgctaa cttccgttac tcgttctgat
    19501 actccgtatt caaaagggcc tgagggccct ttatgctcag gacctgccgc ccgggtatac
    19561 atttccgggg tatcagtcga tttgtttact taaccgccat gaccaaagcc ggcagcaatg
    19621 tcggcttttt acgtccatat attccactca tccggaactg tgacacagaa cctgaataca
    19681 ggattgatca ccggtgccca caaatggcac attttcagta agattctgaa tgttacattc
    19741 ccccttcatg tgtgacacag ctgtcacaga gaacgaaacg gaaagcacat gaaaaacata
    19801 aaaactcaca ccggactcct tatccacaaa gaacaaacac ggcgcgttcg tcttcatgaa
    19861 acaccggccg cctggtgcca cacacgccgg gaatgctaca gcaaaacgac cggtcgtcgc
    19921 tgcggcagcc ctgacagtct cagcaggctg attctgagca gcattcagcc catagaatga
    19981 gataacccgt atgaatgatg acagacgtct acggtacccg atgtatggaa catttgcttt
    20041 ctggctaacg gcatgcccga cacatggttt tacaccgatg acgcctccgt cacaaaagag
    20101 tgggggcggg aatattgtga caatgtggaa gtgttccctc tgtgctatat gcatgacggt
    20161 cccgttttcc gggggcgcta tattgatgag ctcagcgccg cacggagcag aatgctccat
    20221 atgcaccaca tactgccgga acgaacccgg tacagagaaa atatccatga cgcagaatgt
    20281 atccgtgaaa ttcacgaagc gcccctgcag gctcccggca ttctgcaaaa atacaaacgc
    20341 acaaaatcat aaggctgttc tatgtggata ctgattttct ggctctcatc cccctttaat
    20401 gtctcctccg gcgatgcccg gcaacccaca ctggccgttc acagtcagga gttccgtact
    20461 gaaaaagcct gtcgggatgc cttcagcgcg ctgaaaaaag aaaacgtggg agaattcgct
    20521 ctccggggcg tctgcgtccc cggcggagaa taaaccggag atgatgattc tccacaggag
    20581 tattcaatgg cataccattc cccggatgtc acattgcatt atccgtatct gcgccggatg
    20641 tcctgggcgc agataacggc agcagctgaa gatgcggaaa tacatcatga ttatgcccgg
    20701 gcattaatat tgtggcagca tgcatatcat gctgccacgc tgactctcaa caaaaacctg
    20761 gcagttgcta aaatcgactt ctgtattaaa cgtatccgga tgcatgaaca aatgagcagg
    20821 ataatccgga agactggcac agacgaaata ctcttccgtc tgagtaaaca ccatcattta
    20881 tatgagaaaa agaaaacaaa agaggggtga gaaacccatt gtatggggaa aacttgccat
    20941 gctcactgcg ggggcatttt tgtactggct gttctaaccg ggtaacccgt tgatacacat
    21001 agtacaataa tgttttatgc aggcttacgc gccagactgc catcagtagt gcattgcgtg
    21061 ccagctcgcg gacaggggca gtgaccagcc accacatgag taagcatcat ttcagcgcca
    21121 tcaggcagct cctgaaactc ctgcgagttg aaaaagcatc ctcctgcatt agcaatactg
    21181 gcttgtcagt gccagtgtga tataagacgt aatgtcaaag ttcgtggtgt tgacatattt
    21241 ctgtcgcaac caaagtatgc tccctgaggt agcctgagtt taacggacac tccttcctga
    21301 aatagaatgg catcagaagg agctaataat gagcagaaaa acccaacgtt actctaaaga
    21361 gttcaaagcc gaagctgtca gaacggttct tgaaaatcaa ctttcgatca gtgaaggcgc
    21421 ttcccgatta tctcttcctg aaagcacttt aggacaatgg gttaccgccg ccagaaaagg
    21481 gctcggtact cctggttccc gcacggtggc tgaactggaa tctgaaattc tgcaactgcg
    21541 taaggcgtta aatgaagctc gccttgagcg agatatatta aaaaaagcaa ctgtagattc
    21601 aatctgtcaa tgcaacaccc ctttcaatta tctctttcgg tgttttgaac ttcagtgtct
    21661 ttctcggtct gttgtttagc tgagcagcaa ccagatctag ttcatgttga gtatattggg
    21721 caagacatgt ctttttagga aagtactgcc gaattagccc atttgtgttc tcatttgttc
    21781 cccgctgcca aggactctga ggatcgcaga agtaaacttt aacgccggtg ctgacagtaa
    21841 attctagatg tctggccagt tccattcctc tgtcccatgt cagtgatttt ctgagttctg
    21901 acggtaaact caggaatttg tcggtaagag cctgatttac tgagacagaa tctttgcccc
    21961 tgagtctaag gatgatcgta taacgtgatt ttcggtctac aagtgtggct atatgagagt
    22021 tttttgtacc tgagactaaa tcgccctccc aatgccccag agagcgtctg ttatcgatat
    22081 ttcgggaacg ttcgtgaatt ggtgttccgt tcactatgtt aatcgtacct ctttcgcctt
    22141 tgcgggtatg acgcctgcca tggcgaaggc tatgcgaccg tcgcagatgc tgtatattca
    22201 ggtggtgtag cgcttcacgg ctacgaaagt acagcgtttt ataaattgtc tcaggtgata
    22261 ttcgcagcgt tttttgacgt ggttttgttc gccttaacca tcctgatatt tgctctggag
    22321 accatttcat ctccagcttt tccagaacaa gctttcgcaa tggtaaattt tgatccagta
    22381 agcacggttt tggccttttc gccattctgt tggctcggtt attagcatca acagctttgt
    22441 aatagcgtct gccccgatta cgctgaactt cacgtgagat cgtcgaagga ctgcgattca
    22501 gcgcagtagc tatcgcacga atgctcattt tggctgacaa accagctcgt atctcctcgc
    22561 gctcagacag tgtcaggtga gctacagccc gcttacgctc atggggtttt atgccgccag
    22621 tatcccttaa catagtgaag atcgttccgg gttttgaacc caggatattc gctatttcac
    22681 tgaagcctgt tccgttcttc catagttcaa aaacagaggc tttttcctct gctgtaaatg
    22741 ttcgtctcat tcaaaaaacc tccgcaaccc catgttttca cataactgtt gcgttgacca
    22801 attgaatcta caacagcgta ttttgcacag gagtcgctga aaaatacgcg ttaatcgaac
    22861 aatggcgaca acaatttccc attgaagcga tgtgtcaggt atttggtgta tccaggagcg
    22921 gttattacaa ctgggtacag catgaaccct cagacataaa acaaagtgat gagcggctaa
    22981 aactggagat taaggtggca catatccgca ctcgcgaaac atatggaacc cggcggctcc
    23041 agacggagct ggcagagaat ggcatcatcg ttggtcgtga ccgactggca cgtcttcgta
    23101 aggagctaag gctacgctgt aagcagaaac gcaagttcag agcgactacg aactcgaacc
    23161 acaatctgcc agttgcgcca aatctgctga accagacgtt cgctcctaca gcaccaaatc
    23221 aggtctgggt ggcggacctg acgtatgttg ccacacagga gggatggttg tacctcgctg
    23281 gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc catgggagag cgcatgacaa
    23341 aagagctgac aggtaaagcc ctgtttatgg cgctcaggag ccagcgtcca cctgccgggc
    23401 taatccacca ctctgatcga ggttcacagt actgcgcata cgattaccgg gtcatacagg
    23461 agcagtttgg tctgaaaaca tcaatgtcgc gtaaaggtaa ctgttacgac aacgctccga
    23521 tggaaagctt ctggggaacg ctgaaaaatg agagcctgag ccactatcgt tttaataacc
    23581 gggatgaagc catctcagta atacgggaat acattgagat tttctacaat cgtcagcgtc
    23641 gtcactctcg tctggggaat atctccccgg cagccttcag ggaaaaatat catcagatgg
    23701 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc acccgttata
    23761 caccatggat acatggtact ctccgatggt cggtggtggt cgggaagaca cacagaacag
    23821 gctcagtgtg aatactctga tggtgaatac gtattatgac ttcagaaaca gcagtgcatt
    23881 cactccatgg gtgtctgttg gactgggtta tgcacggata catcataagg caacatatac
    23941 tgatacctcc tggaataaat ctggtgaggt gagcgatttt tctgcgttgc attacccggg
    24001 atatgataac aattttgcat ggagttttgg ggcaggtgtc cgctatgacg taaccccgga
    24061 tatcgctctg gacctcagct atcgctatct tgatgcaggt gatgccagtg tgagttacaa
    24121 ggatgagtgg ggcgataaat ataaatcaga agttgatgtt aaaagtcatg acatcatgct
    24181 tggtgtgact tataacttct gacgaaacta ctcctgaaag ataataatta cctgtcttct
    24241 gtaattaaac ggaaactccc tgccgtaatt atactgcagg gttgtctgtt atctgcttta
    24301 caggatattt ctgtttttct tattatttta ttctcttcat aataatgttg ccgatatact
    24361 ccgatgaata tcagaaaact gttttgtccg gacaacacac cacggatttt attgttttta
    24421 ttcttttttg ttatttctgt cataaccaca attgcatgcg gatacactga gaagaatgcc
    24481 accgggaatg tgttgctgct gttccttctt ctgctccttg cacacagaaa tacccttaca
    24541 tccattacag ctctgttatt tctgttctgt tgtgcactgt atgcgcctgc gggtatgacg
    24601 tacggtaaaa tcaacaacag ttttattgtc gcgttgttgc agaccacggc ggatgaggct
    24661 gcggagttta ccgggatgat tcctgtttat cattttctgg tcagtgccgc gattctggtg
    24721 ttcatggtta ttttctggcg gacacaccac cgcggtcacc gtaactggct ggcactgctg
    24781 ctgttcgtat tatgctctgt aaacagctgg ccgttgcgga tggttaaagg aaccgttgtg
    24841 gggacaactg acacattgcg tgaaatgcag cgttatgaac aactgagtca gcacagggct
    24901 gacagctgga aaatcctgcc gggagcaccg ttgtatgaca cgattgtgat tgtcactggt
    24961 gagagtgtgc gcagggatta tatgtcggta tatggttatc ccataccaac cacaccgtgg
    25021 ctgaatacgg ctcccggttt atttattgac ggctatacat cggcagcagc cagtaccgta
    25081 ccttccctga gccggacact gatttatgac tatgagcaga accctgattc cggcaacaac
    25141 gtggtggcgc tggcagcaaa agcaggatac agcacatggt ggatatccaa tcagggaaaa
    25201 ctgggagagc atgacacacg catctctgtt attgcttctg atgcggagca taccgttttc
    25261 ctcaagaaag gcagctttgc ttcccgtaaa acggatgaca tgttgttgtt acaggaaaca
    25321 gaacgtgcac tggcggataa atcctctccg aaggtgatat tcctgcacat gatggggtct
    25381 catccgaacc cgtgtgaccg gcttcactcc tggccgaata attaccagga gcagttcccc
    25441 cggaagattg cctgttacct cgccagcatc agtaaactgg ataactttct cgggcagctt
    25501 gatggtatcc ttcgccggca ttcccgtcat tttgccatgc tttacttttc tgaccatggt
    25561 ctgtcggtca gcgacagtgc taatcctgtt catcatgatg gtcatgtgca gggaggctac
    25621 agcgttcccc tgattattac cgccagtgac atcacgtctc atcagtccgt cagcaggaaa
    25681 atcagtgccc gtcatttcgc aggtattttt cagtggctga ccggcattcg tactgaaaat
    25741 ataccgccat tcaatccgct gacagacgaa gataatgaac ccgttatggt ttttaacggg
    25801 gagaggaatg taccggcaga cagtctgaaa ccgcagcctc tgattcttcc ggtcagggga
    25861 aaataagcgt aatgttcatc accagattat gtacgatgag ttttccactg tttatatcga
    25921 ttatcttgct aattaccaga ctggagtaac cagaccaacc gaaaacaccg gggaggtgat
    25981 tacccgctgg cagaggaaat ccggtttttt tgatgtcagc cataacgtgt ttgacaataa
    26041 agcattatga aagggtgtta atgagtccat gggataattc ataaaaataa tttactgtgt
    26101 tatccttttc aaaagaaata ttcagaagat aacccctggc tgacaaccag gggaagggga
    26161 cattaacagt gttattattg gtggataaat tattacttgt gtgcaggata cactaatgca
    26221 atatctactg atactgtatg ccagtatgtt tcatgcctgc cgctgaaaac ggcaatatcc
    26281 agggccagac atattcacca ttcatatttc tgcatttctg tacaattttc tcagaaagcg
    26341 tctcatcaat tctttcacgc aacttctcct gctgtcttaa aatactttcg catatttcga
    26401 aatcatcttc ctgcatcagc atttcggcgc atttaatgat ttctgcccgt tcatgcagca
    26461 cagaggaatg attatagagc cagtttacaa acagcaatgc ttccggatat tgcccccata
    26521 acgggtggta tgtggcataa taatcttcgg cagtatccgg accgcctgaa aagttatgcc
    26581 atacagcaag tggaaatgga tgcagatttc cgcacagtat ttctgcgatg taagtatttg
    26641 ccgcaaagcc gcgacgaaat gcagttgcag cttttaccag ttccccatta agaaaatgca
    26701 gaaggccgag ttcataccag taaggtgaaa attcatctgc atgttcctgc agtatatggc
    26761 gtgcctgttc atgagcgcca gtgcgtaaca gttccggtcc cagtagccag cgtgcaccat
    26821 gattatcttc gggattgtaa tccagtattt tttcaatgag catgatggca tcctgatgac
    26881 gccgtaaatg tgcatttgcc aggattgccg catagagggc acgaagaaaa ggtcggttgt
    26941 caggatgtat ccagatgatt ctgccactga aaccttcagg gataagacga ttgccaactg
    27001 ccagcccttt aagggcagca ttcagcgcct tacgcggggc attctgctcc agaaatacgt
    27061 atgccaagtg agcgtgtaca tcaataaagt cattctcctg tcgggcaagg cgcctgagtt
    27121 tgttgatgta acttgtttcg ctgatttcat ccgcatcgta tgcatcaatc agttcttcaa
    27181 actcatccag caacgagcca aaccaggttt ccggaaatat gaaacagccc tggttatcgt
    27241 tcacttcaaa gcgtaatttg ccagtcatat tctgaacctg taaaaaagga tggaccataa
    27301 tctgcaggct ataaaaattg tgatgcctgg catcggtgtc cttttattgt ccgggattaa
    27361 cgttgcccat gataatacag tgaatcctgt tctgtggtaa gacgttgagt tattgaatga
    27421 gaacctcagc ctggctgatg gttccgccgg cataaaaaag aaattgattg aggcgggtgc
    27481 tcttcccctt aagaggaaga gcatgtgtgt aaatggtgtt cagtcacctt ttcagtggat
    27541 tgttaacttt tgcatattca aacggactga tacactcctc cctgtttgca tccagaaaat
    27601 ctgcccacca ttgcagcata agccgacgtt cttccagatg ttctgcttta tgaatatacg
    27661 cggcacgtac tccgttgcgc tcctgatgac tcatctgacg ttcaaccgca tctcttgacc
    27721 ataagccgga ttcaatcaat gcactgcacg ccatcgcccg gaatccatgg ccacagactt
    27781 ccttggttgt gtcatacccc atcactctga gggccttatt tacggtattt tcactgattg
    27841 gtttacgcgg gtcatgtgct cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa
    27901 gttctgtcag tatggctaca gcctgtttgc tgagtggcac aagatgtggt gttcgcatct
    27961 tggagccccg gtgtgagaat tttacccctg gcagaggttt gcgttcggct ggaatagtcc
    28021 acatggcatt gtcaatatca atttcagacc agcgggcgta tctgagttca ctggaacgaa
    28081 taaaaaccag taaattcagc ataacggcaa gccgggtgac aggctgacct ttataactgt
    28141 taatacgcga cagaagttca ggcagacgtg aaagatcaag agcggggcga tgctggcgtt
    28201 ttaccgtagt caatgcgcca gccatatcca gagcaggatt atatctgatg atgccagact
    28261 gtacggcata acgcattacg gcagatattc tttgctgcag acgactggcg atttcataaa
    28321 tttgtttagc ttctgcagca cgaacaggga taagcagatc cggggtattg agagttgtga
    28381 tatcccggtt gccgataaag gggaaaacgt gtgtttcgag gcttttgagt actttatcgc
    28441 tgtgtgattg tgcccattgt ttattactgg catgccagcg tcgtgcaata tgttcaaaag
    28501 agagctgtaa atcagggact acttttttag ctcttttctg ttcgcaaggg tcaatcccct
    28561 ccgaaatcag ttttcgggcc tcatcccgtt ttgtacgagc ttcggagaga gaaacctccg
    28621 ggtatacacc aagtgccaga gtcttctcct tacccagaat acgataacgg agacgccagt
    28681 aacgggaacc attaggatgt accagcagga acatgccagc cccgtcagtg agtttataag
    28741 ccttgtcagt aggctttgct gcccggattt ttgcgtcagt cagtgccata gttcagcctt
    28801 ctgaatgcgt taaagagggt acaaattctg tcgaactggg gtatgcccac aattataccc
    28861 acacaatgag gttgatgtgg ggggaatctg gttgagttcg gtagaattga tttggaggca
    28921 ggacgcttaa atcgtggcgt cctggaa
//
LOCUS       DAFATA010000051        28422 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFATA010000051 DAFATA010000000
VERSION     DAFATA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.052"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..401
                     /locus_tag="LGJ20_002697"
     CDS             <1..401
                     /locus_tag="LGJ20_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBK2386733.1"
                     /translation="NLQDSTLIAPSDGTLLTRAVEPGTVLNEGGTVFTVSLTRPVWVR
                     AYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQIGFVSPTAEFTPKTVETPDLRTDLVY
                     RLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            394..2130
                     /locus_tag="LGJ20_002698"
     CDS             394..2130
                     /locus_tag="LGJ20_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2386734.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            2123..3256
                     /locus_tag="LGJ20_002699"
     CDS             2123..3256
                     /locus_tag="LGJ20_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2386735.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            3267..4373
                     /locus_tag="LGJ20_002700"
     CDS             3267..4373
                     /locus_tag="LGJ20_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2386736.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4335..4745)
                     /locus_tag="LGJ20_002701"
     CDS             complement(4335..4745)
                     /locus_tag="LGJ20_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBK2386737.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            4878..5639
                     /locus_tag="LGJ20_002702"
     CDS             4878..5639
                     /locus_tag="LGJ20_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK2386738.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            5636..6877
                     /gene="clsB"
                     /locus_tag="LGJ20_002703"
     CDS             5636..6877
                     /gene="clsB"
                     /locus_tag="LGJ20_002703"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBK2386739.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            6877..7833
                     /locus_tag="LGJ20_002704"
     CDS             6877..7833
                     /locus_tag="LGJ20_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBK2386740.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(7869..8582)
                     /locus_tag="LGJ20_002705"
     CDS             complement(7869..8582)
                     /locus_tag="LGJ20_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK2386741.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(8652..9299)
                     /locus_tag="LGJ20_002706"
     CDS             complement(8652..9299)
                     /locus_tag="LGJ20_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK2386742.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            complement(9501..10205)
                     /locus_tag="LGJ20_002707"
     CDS             complement(9501..10205)
                     /locus_tag="LGJ20_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK2386743.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(10342..10794)
                     /gene="moaE"
                     /locus_tag="LGJ20_002708"
     CDS             complement(10342..10794)
                     /gene="moaE"
                     /locus_tag="LGJ20_002708"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBK2386744.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(10796..11041)
                     /gene="moaD"
                     /locus_tag="LGJ20_002709"
     CDS             complement(10796..11041)
                     /gene="moaD"
                     /locus_tag="LGJ20_002709"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBK2386745.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(11034..11519)
                     /gene="moaC"
                     /locus_tag="LGJ20_002710"
     CDS             complement(11034..11519)
                     /gene="moaC"
                     /locus_tag="LGJ20_002710"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBK2386746.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(11522..12034)
                     /gene="moaB"
                     /locus_tag="LGJ20_002711"
     CDS             complement(11522..12034)
                     /gene="moaB"
                     /locus_tag="LGJ20_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBK2386747.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(12056..13045)
                     /gene="moaA"
                     /locus_tag="LGJ20_002712"
     CDS             complement(12056..13045)
                     /gene="moaA"
                     /locus_tag="LGJ20_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBK2386748.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(13033..13177)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            13442..14350
                     /gene="yvcK"
                     /locus_tag="LGJ20_002713"
     CDS             13442..14350
                     /gene="yvcK"
                     /locus_tag="LGJ20_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBK2386749.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(14542..16563)
                     /gene="uvrB"
                     /locus_tag="LGJ20_002714"
     CDS             complement(14542..16563)
                     /gene="uvrB"
                     /locus_tag="LGJ20_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBK2386750.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(17142..17819)
                     /gene="bioD"
                     /locus_tag="LGJ20_002715"
     CDS             complement(17142..17819)
                     /gene="bioD"
                     /locus_tag="LGJ20_002715"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBK2386751.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(17812..18567)
                     /gene="bioC"
                     /locus_tag="LGJ20_002716"
     CDS             complement(17812..18567)
                     /gene="bioC"
                     /locus_tag="LGJ20_002716"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBK2386752.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(18554..19708)
                     /gene="bioF"
                     /locus_tag="LGJ20_002717"
     CDS             complement(18554..19708)
                     /gene="bioF"
                     /locus_tag="LGJ20_002717"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBK2386753.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(19705..20745)
                     /gene="bioB"
                     /locus_tag="LGJ20_002718"
     CDS             complement(19705..20745)
                     /gene="bioB"
                     /locus_tag="LGJ20_002718"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBK2386754.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            20832..22121
                     /gene="bioA"
                     /locus_tag="LGJ20_002719"
     CDS             20832..22121
                     /gene="bioA"
                     /locus_tag="LGJ20_002719"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBK2386755.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARHRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            22180..22656
                     /locus_tag="LGJ20_002720"
     CDS             22180..22656
                     /locus_tag="LGJ20_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBK2386756.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            22808..24091
                     /locus_tag="LGJ20_002721"
     CDS             22808..24091
                     /locus_tag="LGJ20_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBK2386757.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(24233..26494)
                     /locus_tag="LGJ20_002722"
     CDS             complement(24233..26494)
                     /locus_tag="LGJ20_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBK2386758.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(26677..28110)
                     /locus_tag="LGJ20_002723"
     CDS             complement(26677..28110)
                     /locus_tag="LGJ20_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBK2386759.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(28186..>28422)
                     /locus_tag="LGJ20_002724"
     CDS             complement(28186..>28422)
                     /locus_tag="LGJ20_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBK2386760.1"
                     /translation="CHRALAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGA
                     LDVHLSNEGQDATTLRASVIRTTRKIFSGEVYLP"
BASE COUNT         6774 a         7586 c         7289 g         6773 t
ORIGIN      
        1 tgaatttaca agactcaacg ttgatagcac cgtctgatgg cacgctgtta acgcgcgcgg
       61 tggagccagg cacggttctc aatgaaggtg gcacggtgtt taccgtttca ctaacgcgtc
      121 cggtgtgggt gcgcgcttat gttgatgaac ggaatcttga ccaggcccag ccggggcgca
      181 aagtgctgct ttataccgat ggtcgcccgg acaagcccta tcacgggcag attggtttcg
      241 tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac gccggatctg cgtaccgacc
      301 tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga tgcgttacgc cagggaatgc
      361 cagtgacggt acaattcggt gacgaggcag gacatgaatg atgccgttat cacgctgaac
      421 ggcctggaaa aacgctttcc gggcatggac aagcccgccg tcgcgccgct cgactgtacc
      481 attcacgccg gttatgtgac ggggctggtg gggccggatg gtgcaggtaa aaccacgcta
      541 atgcggatgc tggcgggatt actgaaaccc gacagcggca atgccacggt gattggcttt
      601 gatccgatca aaaacgacgg cgcgctgcac gccgtgctcg gctatatgcc gcagaaattt
      661 ggtctgtatg aagatctgac ggtgatggag aacctcaatc tgtacgcgga tttgcgcagc
      721 gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc tggagtttac atctcttggc
      781 ccgtttaccg gacgcctggc gggcaagctc tccggtggga tgaaacaaaa actcggcctg
      841 gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg atgaacccgg cgtcggcgtt
      901 gaccctatct cacggcgcga actgtggcag atggtgcatg agctggcggg cgaagggatg
      961 ttaatcctct ggagtacctc gtatctcgac gaagccgagc agtgccgtga cgtgttactg
     1021 atgaacgaag gcgagttgct gtatcaggga gaaccaaaag ccctgacaca aaccatggcc
     1081 ggacgcagct ttctgatgac cagtccacac gagggcaacc gcaaactgtt gcaacgcgcc
     1141 ttgaaactgc cgcaggtcag cgacggcatg attcagggga aatcggtacg tctgatcctc
     1201 aaaaaagagg ccacaccaga cgatattcgc catgccgacg ggatgccgga aatcaacatc
     1261 aacgaaacta cgccgcgttt tgaagatgcg tttattgatt tgctgggcgg tgccggaacc
     1321 tcagaatcgc cgctgggtgc aatattgcat acggtagaag gcacacccgg cgagacggtg
     1381 atcgaagcga aagaactgac caagaaattt ggtgattttg ccgccaccga tcacgtcaac
     1441 tttgccgtta aacgtgggga gatttttggt ttgctggggc caaacggcgc gggtaaatcg
     1501 accaccttta agatgatgtg cggtttgctg gtgccgacct ctggccaggc gctggtgctg
     1561 gggatggatc tgaaagagag ttccggtaaa gcgcgccagc atctcggcta tatggcgcaa
     1621 aaattttcgc tctacggcaa cctgacggtc gaacagaatt tacgcttttt ctctggtgtg
     1681 tatggcttac gcggtcgggc gcagaacgaa aaaatctccc gtatgagcga ggcatttggc
     1741 ctgaaaagta ttgcctcaca cgccaccgat gaactgccat taggttttaa acagcggctg
     1801 gcgctggcct gttcgctgat gcatgaaccg gacattctgt ttctcgacga accgacgtcc
     1861 ggcgttgatc ctctcacccg ccgtgaattt tggctacata tcaacagcat ggtagagaaa
     1921 ggcgtcacgg tgatggtcac cacccacttt atggatgaag cggaatattg cgaccgcatt
     1981 ggcctggtgt accgtgggaa attaatcgcc agcggcacgc cggacgattt gaaagcacag
     2041 tcggctaacg atgagcaacc cgatcccacc atggagcaag cctttattca gttgatccac
     2101 gactgggata aggagcatag caatgagtaa cccgatcctt tcctggcgtc gcgtgcgggc
     2161 gctgtgcgtc aaagagacgc ggcagatcgt tcgcgatccg agtagctggc tgattgcggt
     2221 agtgatcccg ctgttgctgc tgtttatttt tggttacggc attaacctcg actccagcaa
     2281 gctgcgggtc gggattttac tggaacagcg tagcgaagcg gcgctggatt tcacccacac
     2341 tatgaccggt tcgccctaca tcgacgccac catcagcgat aaccgtcagg aactgatcgc
     2401 caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt ccggtggatt tcgccgaaca
     2461 gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc accgacggca gtgagccgaa
     2521 taccgctaac tttgtgcagg ggtatgtcga agggatctgg cagatctggc aaatgcagcg
     2581 agcggaggac aacgggcaga cttttgaacc gcttattgat gtacaaaccc gctactggtt
     2641 taacccggcg gcgattagcc agcacttcat tatccccggt gcggtgacca tcatcatgac
     2701 ggtcatcggc gcgattctca cctcgctggt ggtggcgcga gaatgggaac gcggcaccat
     2761 ggaggctctg ctctctacgg agattacccg cacggagctg ctgctgtgta agctgatccc
     2821 ctattacttt ctcgggatgc tggcgatgct gctgtgtatg ctggtgtcgg tgtttattct
     2881 cggcgtgccg tatcgcgggt cgctgctgat tctgtttttt atctccagcc tgtttttact
     2941 cagtaccctg gggatggggc tgctgatttc cacgattacc cgcaaccagt tcaatgccgc
     3001 tcaggtcgcc ctgaacgccg cgttcctgcc gtcgattatg ctctctggct ttatttttca
     3061 gatcgacagt atgcccgcgg tgatccgcgc ggtgacgtac attattcccg ctcgttattt
     3121 cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt ccagtggtgc tggtggtaaa
     3181 cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc ctgacgtggc tgaaaaccaa
     3241 acgtcggctg gattagggag aagagcatgt ttcatcgctt atggacgtta atccgcaaag
     3301 agttgcagtc gctgctgcgc gaaccgcaaa cccgcgcgat tctgatttta cctgtgctta
     3361 ttcaggtgat tctgttcccg ttcgccgcca cgctggaagt gactaacgcc accatcgcca
     3421 tctacgatga agataacggc gagcattcgg tggagctgac ccaacgtttt gcccgcgcca
     3481 gcgcctttac tcatgtgctg ctgctgaaaa gcccacagga gatccgccca accatcgaca
     3541 cacaaaaggc gttactactg gtgcgtttcc cggctgactt ctcgcgcaaa ctggatacct
     3601 tccagaccgc gccgttgcag ttgatcctcg acgggcgtaa ctccaacagt gcgcaaattg
     3661 ccgccaacta cctgcaacag atcgtcaaaa attatcagca ggagctgctg gaaggaaaac
     3721 cgaaacctaa caacagcgag ctggtggtac gcaactggta taacccgaat cttgactaca
     3781 aatggtttgt ggtgccgtcg ctgatcgcca tgatcaccac tatcggcgta atgatcgtca
     3841 cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct cgatcagcta ctggtttcgc
     3901 cgctcaccac ctggcagatc ttcatcggca aagccgtacc ggcgttaatt gtcgccacct
     3961 tccaggccac cattgtgctg gcgattggta tctgggcgta tcaaatcccc ttcgccggat
     4021 cgctggcgct gttctacttt acgatggtga tttacggttt atcgctggtg ggattcggtc
     4081 tgttgatttc atcactctgc tcgacacaac agcaggcgtt tatcggcgtg tttgttttta
     4141 tgatgcccgc cattctcctt tccggttacg tttctccggt ggaaaacatg ccagtatggc
     4201 tgcaaaacct gacgtggatt aaccctattc gccactttac ggacattacc aagcagattt
     4261 atttgaagga tgcgagtctg gatattgtgt ggaatagttt gtggccgcta ctggtgataa
     4321 cggccacgac agggtcagcg gcgtacgcga tgtttagacg taaggtgatg taacttctta
     4381 tctttcgcca gcaaagacac taccgccggg ccggcaagga ttgccagccc tgcaattgcc
     4441 agcaaaaagt tgttttccag tacacgggac aacagccaca gcacaatcag cgctgcgcca
     4501 aaataccagg tcgtggtcag ctcttccagc acgtctgcca cctcacgcca gcgttgctcg
     4561 gggtggcgct gaaacaccaa catcatcact accgtgacac aatacagtgc gcataatccc
     4621 acgccgacgt gaaccagagt cttactcatc cagcccacca ccagcagcgc cactaccagt
     4681 aaatgcaaca taatctgcca gcaacttaga ccagttgcga cacgaacacg ttgttgccac
     4741 ttcatggctt ctctcctgaa gaatttatct ctacttactc agagtaccga actttacgga
     4801 aaattccgta agaccgcacc tttttgtgac gccgactaca ctatttcaat gagataagag
     4861 gcaaagacag gaaaattatg cccgatcaaa cacaacaatt ttcgttcaag gtgctcacca
     4921 tcaacattca caaaggtttt accgcgttta accgacgctt cattttgccg gaacttcgcg
     4981 acgcggtgcg taccgtcagc gccgatattg tttgcctgca ggaagtgatg ggcgcgcacg
     5041 aagttcatcc gctgcatgtg gaaaactggc ccgatacctc gcactacgag tttctcgccg
     5101 acacgatgtg gagcgatttt gcctacggtc gcaatgccgt atacccggaa gggcatcacg
     5161 gcaacgccgt actgtcgcgt tatcccattg aacattatga gaatcgcgat gtttcggtcg
     5221 atggtgcgga aaagcgcggc gtgctctact gccgcattgt gccaccgatg accggaaaag
     5281 cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc gcaccgtcag gcgcagcttg
     5341 cgatgctcgc cgaatgggtg aatgagctac cggacggcga accggtattg gtggcgggtg
     5401 atttcaatga ctggcggcaa aaagctaatc atccgttaaa agtgcaggcc ggactggatg
     5461 agatttttac ccgcgtccac ggacgcccgg cgcgcacgtt tccggtgcaa tttcctctac
     5521 tacgactgga caggatctac gtcaaaaatg ccagcgccag cgcgccaacc gcgttgccgc
     5581 tgcggacatg gcgacacctt tctgatcatg cccctttaag tgcggagatt catttatgaa
     5641 atgtagctgg cgcgaaggca ataagatcca gttgctggaa aacggcgagc aatattatcc
     5701 cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc attcttgaaa cgtttatctg
     5761 gtttgaggat gacgtcggca aacagctgca tgcggcacta ctggcagcag cgcaacgcgg
     5821 ggttaaagcg gaagtcttgc tggatggcta cggttcgccg gatctcagcg atgagtttgt
     5881 caatgaactg acggcagctg gcgtggtatt tcgctactac gatccccgcc ctcgcctttt
     5941 tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa attgtggtga tcgacgcgcg
     6001 tatcgccttt attggcgggc tgaattactc cgccgagcat atgtccagct acggtccaga
     6061 ggctaaacag gattacgcgg tacgccttga agggccgatt gtcgaagata ttctccagtt
     6121 tgagctggaa aacctgcctg gacagagcgc ggcacgacgc tggtggcgac gtcatcacaa
     6181 agcggaagag aaccgccagc cgggagaagc gcaggtattg ctggtctggc gcgataacga
     6241 agaacatcgc gatgatattg aacgtcacta tctgaaaatg ctcactcagg cgcggcgaga
     6301 agtcattatc gccaacgcct acttcttccc cggctatcga tttttacacg ccttgcgtaa
     6361 agcggcacgg cgcggggtgc ggatcaaact gatcattcag ggcgaaccgg atatgccgat
     6421 tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt aaaggcggcg ttcaggtatt
     6481 tgagtaccgc cgccgcccgc tccacggcaa agtggcattg atggacgatc actgggcgac
     6541 agtagggtcc agtaatctcg atccgctcag tttgtcactg aatctcgaag caaatgtcat
     6601 catccacgat cgtcatttta accagacgct gcgcgataat ctgaacggca ttattgccgc
     6661 agattgtcag caggtcgatg agaccatgct gccgaaacgc acctggtgga acctgaccaa
     6721 aagcgtgctg gcgttccact ttttacgcca cttcccggcg ctggttggct ggcttccggc
     6781 acacacacca cgtctggcgc aggttgatcc gcccgcacaa ccgacaatgg aaacgcagga
     6841 tcgggtagaa actgaaaaca cgggggtaaa accctgatga gtaaatcaca cccgcgctgg
     6901 cgcttagcaa agaagatcct cacctggctg ttttttatcg cggtgattgt gttgctggtg
     6961 gtctacgcca aaaaagtgga ctgggaagag gtctggaagg tcatccgcga ctacaatcgc
     7021 gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct atctgattta cggctgctat
     7081 gacctgctcg cccgctttta ctgcggtcac aaactggcga agcgccaggt gatgctggtg
     7141 tcgtttatct gctacgcctt caacctgacg ctcagtacct gggtcggcgg cattggtatg
     7201 cgctatcgtt tgtactcccg actagggtta ccgggcagca ctattacgcg gattttctcg
     7261 ctcagtatta ccaccaactg gctgggctat attttactgg cagggattat ctttaccgca
     7321 ggcgtggtgg agttgccgga tcactggtat gttgatcaaa ctacgctgcg tattctcggc
     7381 attggcttac tgatgattat cgcggtttat ttgtggtttt gcgctttcgc gaagcaccgc
     7441 catatgacca tcaaagggca aaagctggtg ctgccttcat ggaaattcgc cctcgctcag
     7501 atgctgattt ccagcgttaa ctggatggta atgggggcga ttatctggct gttacttggt
     7561 caaagcgtga actatttctt tgtactgggc gtgttactgg ttagtagtat tgctggcgtc
     7621 atcgtgcata ttccggcggg gatcggtgtg ctggaagcgg tgtttatcgc actactggct
     7681 ggggagcata cctcgaaagg gagcattatc gccgccctgc tcgcttatcg cgtcctgtat
     7741 tactttatcc cgctgctgct ggcgctgatc tgttacctgc tactggaaag ccaggcgaag
     7801 aagctacggg cgaaaaatga agcggcgatg tgaagtttag ttaagttctt tagtatctgc
     7861 atttacgact aatgaaaaaa acgcgtatgc ctttgccaga caagcgttat agctatcgcg
     7921 gcaagtatcc caaagaagcc gataaaatag taatagagtg ttaatgcacc tgtaacagcg
     7981 caacgaaccg gactccacaa ctcttcacta tggcatttcc ccgccaattc aaggagctta
     8041 tttcgtccat gcgagataat gcctgaccat aacacaatca ttaagcaact tattacccaa
     8101 acgggtcgtt cactcattaa tgcggcattc atgatgatca ccagggccaa ccctgtcaac
     8161 gtcatcataa tgataaaacg gtgagaacca ggatcaacat taaataataa accggccagc
     8221 atactgatta aaaacatcgc gcccgcagtg ctgaacacga cgataatggc aggtacagga
     8281 gctaaacttg caacgacaat cgccaccaaa gagagtaaaa acagatatat tacgcctgcg
     8341 atgacggcat ttatcttgtt gatatcctta cttaagccat acatacaaac taataataac
     8401 cctatggcga catacattgt tggtgaatca tatttggttg ccgtaatttc gagatactta
     8461 gctccccagg ccagtcctgc ggttgccacc aaacctaaag cggtaatgag ccatatgctg
     8521 tttaatatcc tggcttcgtg ctgaaaatca ttagttatgt tatttgctga gcgcgaattc
     8581 attattttta tatccttaag ttaaatatca ttatctattc caattaacgc aataaaactc
     8641 gtcaattcgc actaccacac taaaccatta ataaattcaa gaactgttac agtaattcta
     8701 aagaggacgc ttaaaatact aaaatagagt gttattgccc caggaacgat acagcgaaga
     8761 gaacctgaaa tttcagaggt gtcggcaaca cggatcaaat cttccagtgt tgttgcttca
     8821 caggcagccg taatgcccca gataaagaca gtcaaaatac tcgctaacca cacggaaaaa
     8881 ctgctggtaa ggatcaggtt aacaacaaaa gcaatactca aaccacagaa aatatatgaa
     8941 tataactgtc tgactggtgg gataacccta ttactacaag agcaaatcag cgccattgcg
     9001 gcaaacatcc ctcccgtaat tcccattacc gtcgctatgc tcgaaaaggt ataaaggctg
     9061 gcgaagagaa agccaacaag aaggattatt agcagataga ccagacatgc gcctgataca
     9121 ttcagtcttt tcagggcgtt gtttaaccag agaccaccta ttatcagcgc agccaaagcg
     9181 aagacaaacg cacctggaga cttttgtgac gtaacccaat gcaaatgctg aaatatccag
     9241 gcacagcctg ccgtagccca tactccagct gctgttaacc accaaatgag aagcagcatt
     9301 ttgctgagcg aagcactttt ttgctgtaat tgagtagcag tggattgtct gttcaaatgc
     9361 gttatctgtc cctgaaatta cgtagtggac atagtctggc atttgccgcg attcagttct
     9421 catgtaaatg agcggaatag tctttataaa atccgaaaaa aatcccggct ccaggccggg
     9481 atcagcagga agaagagaat ttaacggcgg ttgccgaaga tccgcaacaa catcaggaac
     9541 aggttgatga agtccagata caaggttaac gcgccaagaa tggaatattt gcgcaggttc
     9601 gacgtgtcgc gggtatcaat ctgctcaccc atatttttca gtttctgcgt gtcatacgcc
     9661 gtcaatccga caaagacaat cacgccgatg taggtaactg cccacatcaa tgcttcgctt
     9721 ttcaaccaga agttgaccag cgatgccagc acaatgccga ttaacgccat aaacagcata
     9781 ttgccaaagc cacttaaatc gcgcttcgtg gtgtaaccgt acaggctcat tgcgccgaac
     9841 atcccggcag taacgacgaa agtactggcg atagaagcag cggtatagac aatgaatata
     9901 ctggaaagcg taagacccgt cagcgccgaa taaagcataa agagcatcgt cgttacacct
     9961 gcgctcagct tttgaatcat cgctgataac acaataacca atgctaattg cgcgatgatc
    10021 agaccgatta aaaagacacg gttagtgaac aacagctcca tcacggccgc ggaattagcc
    10081 gcataccagg caacaaatgc ggtcagcaac aagccaacgg tcatccaacc atagacttgc
    10141 gccatataag tttgcaagcc agcccggggt tgtacgattg aatcagaacg tgggaatctg
    10201 tccatgacga tctcctgaag atataaggaa tatcttaagg atactgcaaa atgatgaggc
    10261 tgtgcatcga cgcagcgtaa acgtatgtac tgagcggtga aattgccgga cgcagcggtg
    10321 ccttatccgg ctaacaaaaa actaccagcg ttttgccgcc tgctgatcgc tctcccgggc
    10381 ttcaacccag cggtcgcctt ccggcgtggc ttcgcgcttc cagaacggtg cgcgggtttt
    10441 gagataatcc ataataaact gcccggcttc aaacgcactg ctgcgatgcg cactggtgac
    10501 accgacaaaa acgatttcat cgcccggcca taattccccg atgcggtgaa tcacagtgac
    10561 gcgccccagc ggccagcggt tacgcgcttc atcaacaatt tctgccagtg ctttttcagt
    10621 catccccgga tagtgttcga gggttaatgc tttgacgctg tcgcccaggt tatggttgcg
    10681 caccttacca gtaaaggtga ctaccgcacc gtcttcgtca cgctccgcca gccacgggta
    10741 ctcttctcct acgctgaacg gctgcggacc aacaacaatt ttggtttctg ccatcttaac
    10801 ctccggttac cggcgggaag aaagctactt cgtcgccgtc agtcagcgga tggtcaaaac
    10861 tcaccagcgt ctggttgacg gcagccagta atttgccatc ttccagcgcc agcgcccagc
    10921 gatcgctctg cgcagccatg tgctggcgta acgcttcaac agttgggaaa tccgcagcca
    10981 cttcggttgc atctgttccc accaactcgc gcacctgggc gaaaaaaaga actttaatca
    11041 tccgcttcca ccttaaagtc acccgacttg ccgccgctct tcgccagcaa acgtaccgga
    11101 ccaatcacca tatctttttg caccgctttg cacatgtcat aaatggtcag cgccgccacg
    11161 gaggccgcgg ttaatgcttc catttcgaca ccggttttcc cggtcaggcg gcataaggtt
    11221 tctatacgca cccgattgtg ctccggctcg gcctgtaaat tgacttcaac tttgctgagc
    11281 atcagcggat gacagagcgg gatcagatcc caggtgcgtt ttgccgcctg aataccggca
    11341 atacgcgcag tggcaaatac gtcgcctttg tggtggcgac catcaataat catcgccagc
    11401 gtctcgctgc gcatggtgac aaaggcttcc gcccgcgctt cacgcacggt ttccgctttg
    11461 gcggagacat ccaccatgtg cgcttcgcca gcggcgttga tatgggtcag ttgcgacata
    11521 cttatttctt caaatgtgga tggaaattac acggacgcgt acgggcatcc agctgcggcg
    11581 cgatgatatt ttcccatgcg gtacggcacg ctttggtcga acccggcatg gcgaaaatca
    11641 gcgttttgtt ggcgacgccc gctaccgcac gagattgcaa cgtggaagtg ccaatctctt
    11701 caaaagacaa catccggaac acttcaccaa aaccttcaac ttcacggtcg aacaacggca
    11761 gcaatgcttc gggagcctga tcaccttccg tcaggccagt accacccgta atcaatacca
    11821 cttgtacatc gtcgctggcg atccacgcag atacctgagc gcgaatagcg tagcggtttt
    11881 ctttcacaat ggctttatcg acaacgtgat ggcccgcttc ttgcgccgaa tcgcgcagat
    11941 agtgaccgga ggtatcgtct tcttcaccgc gacgattaga aaccgtaaga atagcaatac
    12001 gggtcgggat aaattcagtg cttacctgac tcatctgatc tctccttttg acgttttagc
    12061 cgccaatgta cgataagttt tgcgtaatac cggtgttgtt ttgatgcagg aaatgggtct
    12121 gtttcttctc ccgcagcgcc gctgaaatac gcgcttccag cgcctgttgc tgggtatcgt
    12181 cttccagcag atcgcgcagg ttaacgccgc cttcaccaaa caggcagaga tggagtttac
    12241 caatggagga aacgcgcagg cggttgcaag tggcgcagaa gtctttttca tacggcatga
    12301 taaggccaat ctctccggcg taatctggat ggcaaaagac ttgcgcggga ccgtcgctgc
    12361 gttgacgtaa ttggtggatc cagccgcgac gcagtagctc gtcacgcaga acctgaccag
    12421 agatgtgatg cttacggaag agctcgctgc cctcgcccgt ttccatcagt tcgatgaaac
    12481 gcagctggat agggcgatgc tggatccagt tcagaaaggt gtcgagctgg tgatgattaa
    12541 catcacgcat cagcacggta ttgactttga ccttctcaaa accgacctca aatgcagcat
    12601 caatccccgc catgacctgg ttgaatttat cctgcccggt aatagcgtga aactggcggg
    12661 cgtccagact gtcgacactg acgttaatgc cagtaagtcc cgcatcgcgc cagttcgcca
    12721 catcgcgttc cagacggtaa ccattggtgg tgaccgcaat ctggcggata gcgtcgtttt
    12781 cccgcacagc ggcgatgata tcggtaaagt cgcggcgtaa agacggctca ccgcctgtca
    12841 ggcgcacttt ttcggtgccc agactggcga aggcgcgcgt aacccggcga atttcatcga
    12901 cggtaagaaa gcctttattg gtgacgccgc tcggtttgta gccatccggc aggcagtagg
    12961 tgcaacgaaa gttacacaca tcggtaatcg acaggcgcaa gtagtaaaac ttacgcgcaa
    13021 atgcatcagt cagttgtgaa gccatgtaca cctttccaga tacgggaggc gaagtcattt
    13081 cttccttcgc cctggtggca atctttacga gcattgtcac ggccaaagca ccgtatcagt
    13141 tgacccaggt gcagaggcta gagtgtttag tggttatgcc gatactagcg tgtaaatgag
    13201 atttttacca tccacacttt cgctatgtaa tcatgtatat agcgtcatga tcgtgctatt
    13261 tcgctacaga ataagggaaa atggcggttt tactttgata tacatcatca gggaggcata
    13321 gcgtgcatcg tcctttagag gtagaagaaa atagtttgta tcctcaatat tggcaggtta
    13381 attgctgttt ccccgcaatt tgcgctactg tagcgcggat aatctgactc caggaaacta
    13441 tatgcgcaat cgtacgctgg ctgatcttga tcgtgtcgtt gctctcggcg gagggcatgg
    13501 actgggacgc gttctctcat cactttcgtc tttgggttct cgtttaacgg gtatcgtcac
    13561 caccaccgat aatggtggct cgacggggcg tattcgccgt tcagaaggcg gcattgcctg
    13621 gggcgatatg cgcaactgcc tcaaccagct gataacggaa ccgagcgtcg cctccgcgat
    13681 gtttgaatac cgttttggtg gcaatggcga actttccggt cataatctcg gaaacttgat
    13741 gttaaaggcg ctggatcacc ttagcgtgcg gcctctggaa gccatcaatt taattcgtaa
    13801 tctgctgaaa gtggatacgc atttgattcc aatgtcagag catcctgttg atctgatggc
    13861 gattgacgat caggggcatg aagtttacgg cgaggtcaat atcgaccagt taactacgcc
    13921 gattcaagag ttattgttaa cgcctaatgt acccgcaacg cgtgaggcgg ttcacgctat
    13981 caatgaagcg gatctcatca ttattgggcc tggcagtttt tataccagcc tgatgccaat
    14041 tctgctgctg aaggaaatcg cccaggcatt acgccgcacg ccagcgccga tggtttatat
    14101 cggcaatctg gggcgtgagt tgagtttacc tgcggctaat ttgaagctgg aaagcaagct
    14161 ggcaattatg gagcagtatg ttggtaaaaa ggtcattgat gcggtcatcg tcgggccaaa
    14221 agtggatgtc tcggcggtga aagagcggat tgtaatccag gaggtactgg aggccagcga
    14281 tattccgtat cgtcatgacc gccagttgtt acataacgcg ctggaaaagg cgttacaggc
    14341 tttaggttaa cggaaatccg aaggaaaatt ccggcttcct attgaagaca aagtgcgcgt
    14401 tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caaaccgcgc aaattcaata
    14461 tattgcggag aaaatgtagg cctgataagc gtagcgcatc aggctgtttt ccgtttgtca
    14521 tcagtcttct tcgctatcct gttacgatgc cgcgataaac agctcacgca gctgatgcaa
    14581 ctggtcacga atttgcgccg cttcttcgaa ctccagattc tgcgcgtgtt gcatcatcaa
    14641 cccttccagc tcatggattt tctgctgcaa cgctttaggc gacatatcca tcggcacatt
    14701 atccggctca acaatcggtc gcgattttcc tctgcccttc gctttggttt tagcaatgtt
    14761 ctgccccaac gccaggatat cgaccacttt cttgttcaag ccttgcggcg taatgccgtg
    14821 ttcctcgttg tacttctgct gtttctcgcg gcgacgttcg gtttcgccaa tcgctttcgc
    14881 cattgatggg gtgatcttat cgccgtagag aatcgcttta ccgttaacgt tacgtgccgc
    14941 acgaccaatg gtctggatca acgaacgttc ggaacgcagg aagccttctt tgtcagcgtc
    15001 gaggatcgcc accagcgaaa cttccggcat atccagacct tcacgcagta agttgatccc
    15061 taccaatacg tcgaactcac ccagacgcaa gtcgcggata atctccatac gttcgacggt
    15121 gtcgatatct gagtgaagat aacgcacgcg ctcgccatgt tcttcgagat attcggtaag
    15181 atcttccgcc atccgcttgg tcagtgtggt gaccagtacg cgttcgttaa ttgccgctcg
    15241 ctgacgaatc tccgaaagaa gatcatcaac ctgtgtcgcc accggccgca cttcgataat
    15301 cgggtcaagc aatccggttg gacgcaccac ctgatccacc acatcgccgc cggatttttc
    15361 cagctcgtaa ttacccggcg tcgccgaaac atagatggtt tgcggcgcta atgcttcgaa
    15421 ctcttcaaac ttaagcggac ggttatccag cgctgatggc aggcggaagc cgtactccac
    15481 cagtgtctct ttacgcgccc ggtcaccgcg atacatgccg ccaatttgtg gaatggtgac
    15541 gtgagattca tcgaccacca gcagcccatc agcagggagg taatcaaaca gcgtcggcgg
    15601 tggctcaccc ggtccacgac cggagaggaa gcgcgagtag ttttcaatcc ccgaacagta
    15661 gcccagctcg ttcatcatct ccagatcaaa ctgggtacgc tgggtcagcc gctgctcttc
    15721 cagcagtttg ttgttttcca acagcacttt gcgtctggcg gccagctctt ctttgatctc
    15781 ctccatcgcc tgtacgatgc gctcgcgcgg tgtgacgtag tgcgttttcg ggtagatggt
    15841 aaaacgtgga atagtggaaa caatctgccc ggtcagcggg tcaaataacg acaatcgttc
    15901 cacttcctcg tcaaacagtt ccacgcgaag tgcaatgtca tccgattctg ccgggaagat
    15961 atctatcacc tcgccacgaa cgcggaaagt accacgctgg aatgcttgat cattacgagc
    16021 gtattgcagc tccgccagtc ggcgcagaat cgcgcgctga tcgataatca taccgaccgt
    16081 gagatggagc atcatcttga gatataaatc aggatcgccc agaccataaa tcgcggaaac
    16141 agacgccacc acaaccacat cacgccgctc cagcatcgct ttggtggcgg acaaacgcat
    16201 ctgctcaata tgttcgttaa ccgaggcatc tttctcaatg aaagtgtcgg aactcggtac
    16261 ataggcttcc ggctgatagt agtcgtagta ggaaacgaaa tattccaccg cgttttccgg
    16321 gaagaactct ttcatttcgc catacagctg ggccgccagc gttttgttgg gcgcaagtac
    16381 catggttggg cgctgaaggt cagcaatgac attggcaatg gtgaaggttt tcccggagcc
    16441 agtcacgcca agtaacgtct ggtgcgccag gccatcttcc agcccctctt cgagacgtcg
    16501 aatcgcctct ggctgatcgc cagaaggttt aaaagcggaa ttcagtttga acggtttact
    16561 catgagtcgc tacctgaagg agttgggcgg gcaggtatgt aattttactc gtcgtactta
    16621 attatgccaa caaattatac tggataaaaa aacagttcat caccataata tttctgttac
    16681 agcgtaaact ccgctgtcaa tcatgagcaa aatttactct gtggcgagat aaaactccgg
    16741 ccttaccggg ttatccccaa agcaacggct tttttaacat ttgtcaagat gagtgatgac
    16801 agttttatgg caagagatgc ctgttcagtg actgccattg catttatcta accagttaaa
    16861 aaataaaaga tatttcattg agccgtcttt aacgccaatt cgcgtgcaag ccgcgtattc
    16921 tctcgcttgc ctcgtgtttt ctaactctaa tacacatggt tatccacagg aatagtggat
    16981 aactgtctgc agcccctatc ccccgccgcc tgggcaattc ccacattcgc cgcatgatgc
    17041 ggaagtaaaa tttttttcgt gattatttta gaatttaatt ggttaaattg cagtcaatcg
    17101 aagacgcgat ctcgctcgca atttaaccaa atacaggatg gctacaacaa ggcaaggttt
    17161 atgtactttc cagttgccgc attttctgga ttttctgcaa gccaggggat ctctcccagc
    17221 agcggcgcgg gaatcatgcg ggtgagcgtg gtcatatatt cagcgtgacg ttttccagga
    17281 ggcgtgacat cattcgccac ccaacccgcc agagtcagtc cggcgtgttg tattgcctgt
    17341 gcagttaaca tcgcgtgatt aatacagcca agtttcacac caactaccag tattaccggc
    17401 agttgttcct gtgttaccca atctgcaaaa gtgaaagtgt cagaaagcgg cgtaaaccag
    17461 ccgccagcgc cttccactaa cacccagtca gcctgttgtt caagcgcgcg taatccggcg
    17521 ctcattacca atgattctat cggcctgccc tcttgcgcgc tgatgatgtg cggcgaggtg
    17581 ggttctgcga aggtgtaagg atttaccgtt gcgtaatcca gctgcaggct gctgttgcgc
    17641 tgtaacgcca gcgcgtcgct attacgtaag ccttccgggg tcttttcact gccagaggcg
    17701 accggtttat aacctgccgt ccggtagcct actgcctttg cggcttgtaa aagtgcacaa
    17761 ctggcgacgg ttttccccac ttcggtatcc gttccggtga caaaataacg tttactcacg
    17821 agcaatcact cccaaaaaaa gatgatacgt cagaggatat cgcccctgtt gttgcggcca
    17881 agccagttgc aatcgctgca actgcgaacg cgttaatatt cgcgggtcgc gcccttcatg
    17941 aagatgcgtg gcaccaatgc ctttcagcga acgcatggca ctgagcgcat catcaaacca
    18001 cagcgtgatg ggctgaatat gatgttgata atgcacgccg ttcagtgact gttcgatttc
    18061 atctggcggt aaaaagcgat tagcatgcgg acgctcgtcc accgcctgcc acgcctgatg
    18121 cagttcgggt aacgatccct gcaccagcgt ggtaaacgcc accacgcctc cggggcgcac
    18181 cacccgatac agctcgcgga gtgccgtgga taaattaccg caccactgca ctgcgagatt
    18241 gctccatgca agatcgaacg tcgcagtcgc taacggcagg gattcgatat ctcccgccag
    18301 ataatggtct gcggcatcct tctggcgtgc ctgaacaagc attggcggcg agagatctaa
    18361 ggccgtcacc tgcgcgtgac gttcccgcca gtggcggctc atccagccag gtccacaacc
    18421 cgcgtccagt acgtgggtgt atttacgctg tgggagcatt gccagtaaga cgtcagcact
    18481 ctggcgctgt agatctgcgt gttgctcata gagtgcggct gcccgaccaa atgccgctgc
    18541 aatggcttgt ttattaaccg ttgccatgca gcacctccag cagacggtcg atatcctgca
    18601 tttcatgcgc tgcggttagc gttaagcgca gtcgcgcagt accagcgggt acggttggcg
    18661 ggcgaatcgc cgtgacccag cagccttgct ggcgcagttt ttctgccagt tgtaacgcac
    18721 ggctgttatc accgacgatc aatggctgga tggcgctgca tgaatcagcc agcgtaaacg
    18781 gcaaatcctg tactccggca cgaaaacgcg caatgagtga caccagtttt tcgcgccgtg
    18841 catccccctc atcacggcga atgaccgcca gtgacgcacg taatgcctgc gcctgagcgg
    18901 gcggcatact ggtgctgtag ataaggtggc gggcgaattg cagcagataa tccgccaccg
    18961 tactggagca aagcaccgca gccccactga cgccaaatcc tttgccaaaa gtcaccacca
    19021 gcaattctgg ttttaccttt tgcagccagc agctgccgcg cccctgctcc ccgataacgc
    19081 ccgtgccgtg ggcatcatcg accatcaacc agccattgtg ctgttgcgtt acctgctgga
    19141 tttccgccag tggcgcaata tcgccgtcca tgctgaacac gccttctgtc accaccagtt
    19201 gctgccccgg acagggggaa gcaagcagtc gcgccagatg agtgacatcg ttatgaacaa
    19261 aacggcgaag ctgcgacggg cttaaactgg cagcctccag caatgaggca tggctaagcc
    19321 ggtcagcgac aatgcggtcc tcttttgcca tcatcgcggc aatgaccgcc tgattagcgg
    19381 caaaaccaga gataaatagc agtgcccgcg aatagcccag ccactcggcc agttcttctt
    19441 ccagcgcctg atgcgcaacg ctataaccgc tgacgtgacc ggaaccgccg ctaccgatgc
    19501 caaattgctc cgccccctgc tgccaggcac ggataatttg cggatgatgg cttaaaccta
    19561 aataatcgtt actggaaaag ttcagatact ggcgatcatc cgccaccagc cagcgtccgg
    19621 ctccttgcgc cactggataa cggcgacgca gggcatcggc agcacgccgc gcatcgagcg
    19681 ccgcgttgat tttctcctgc cagctcataa tgctgccgcg ttgtaatatt cgtcggtgtc
    19741 cggggtcatc agcgcctgtt caagacgttg ctgttgttcg ttatcccctg ccagcacggc
    19801 agtttgctgc ggatttagcc ccagtttgcg gaacagttgc aggtctttat cttcttccgg
    19861 attcggcgtg gtcagcagtt tgcaaccgta gaaaatcgag tttgcgcctg ccataaagca
    19921 catcgcctga gtctgttcgt tcatctgctc gcgtccggca gaaaggcgca cgtaagaggt
    19981 cggcatcatg atccgcgcga ccgcaatggt gcgaataaaa tcaaaggcat cgacatcatc
    20041 gttatccgcc agcggcgtgc ctttcacctt caccagcatg ttgattggta cgctttccgg
    20101 cggcgtcggc aggtttgcca gttgcagcaa taatccggcg cgatctttta ccgtttcgcc
    20161 taagcccaca atgccgccag aacagacttt gatcccggca tcgcgcactt tttccagcgt
    20221 atcgaggcgt tcctgataag tgcgggtggt gatgatattg ccgtaaaact ccggcgaggt
    20281 gtcgaggttg tggttgtagt aatccagccc ggcgttcgcg aggcgctgcg cctgagattc
    20341 actcaacgtg cccagcgtca tacacgcctc cagccccatc gcttttaccc cctgcaccat
    20401 ttgttccagg tacggcatat cgcgttcgtg gggattcttc cacgccgcgc ccatacagaa
    20461 gcgcgtcgat cctgccgctt tcgctttgcg cgccgactcc agcacctgtt caacttccat
    20521 caaccgctcg gcttccagcc cggttttgta gcgcgagctt tgcgggcagt atttgcaatc
    20581 ttccggacaa gctccggtct taatcgacag caacgtgctg acctgcacct gacgtggatc
    20641 gaaatgctga cgatgcacct gctgcgcttc aaacagcaga tccagcaacg gtttttcaaa
    20701 taattctgtg acttgcgaca atgtccagcg tgggcggtga gccatggggc ttctccaaaa
    20761 cgtgtttttt gttgttaatt cggtgtagac ttgtaaacct aaatcttttc aatttggttt
    20821 acaagtcgat tatgacaacg gacgatcttg cctttgacca acgccatatc tggcacccat
    20881 acacatccat gacctcccct ctgccggttt atccggtcgc gagcgccgaa ggttgcgagc
    20941 taattttgtc tgacggcaga cgcctggttg acggtatgtc gtcctggtgg gcggcgatcc
    21001 acggctataa tcacccgcag cttaatgcgg cgatgaagtc gcaaattgat gctatgtcgc
    21061 atgtgatgtt tggcggtatc acccatgcgc ccgccatcga gctatgccgc aaactggtgg
    21121 caatgacgcc gcaaccgctg gaatgcgttt ttctcgcgga ctccggctcc gtagcggtgg
    21181 aagtggcgat gaaaatggcg ttgcagtact ggcaggccaa aggcgaagcg cgccaccgtt
    21241 ttctgacctt ccgcaatggt tatcatggcg atacctttgg cgcgatgtcg gtgtgcgatc
    21301 cggacaactc aatgcacagc ctgtggaaag gctatctgcc agaaaacttg tttgctcccg
    21361 ccccgcaaag ccgcatggat ggtgaatggg atgaacgtga tatggttggc tttgcccgcc
    21421 tgatggcggc gcatcgtcat gaaatcgcgg cggtgatcat tgaaccgatt gtccagggcg
    21481 caggcgggat gcgcatgtac catccggaat ggttaaaacg aatccgcaaa atgtgcgatc
    21541 gcgaaggtat cttgctgatt gccgacgaga tcgccaccgg atttggtcgt accggcaaac
    21601 tgtttgcctg tgaacatgca gaaatcgcgc cggacatttt gtgcctcggt aaagccttaa
    21661 ccggcggcac aatgaccctt tccgccacgc tcaccacgcg cgaggttgcg gaaaccatca
    21721 gtaacggcga agccggttgc tttatgcatg ggccaacttt tatgggcaat ccgctggcct
    21781 gcgcggcagc aaacgccagc ctggcgattc tcgaatctgg cgactggcag caacaggtgg
    21841 cggctattga agtacagctg cgcgagcaac ttgcccccgc ccgtgatgcc gaaatggttg
    21901 ccgatgtgcg cgtgctgggg gcaatcggcg tagtcgaaac cactcgtccg gtgaatatgg
    21961 cggcgctgca aaaattcttt gtcgaacagg gtgtctggat ccggcctttt ggcaaactga
    22021 tttacctgat gccgccctat attattctcc cgcaacagtt gcagcgtctg accgcagcgg
    22081 ttaaccgcgc ggtacaggat gaaacatttt tttgccaata acgagaagtc cgcgtgaggg
    22141 tttctggcta cactttctgc aaacaagaaa ggagggttca tgaaactcat cagtaacgat
    22201 ctgcgcgatg gcgataaatt gccgcatcgt catgtcttta acggcatggg ttacgatggc
    22261 gataatattt caccgcatct ggcgtgggat gatgttcctg cgggaacgaa aagttttgtt
    22321 gtcacctgct acgacccgga tgcgccaacc ggctccggct ggtggcactg ggtagttgtt
    22381 aacttacccg ctgatacccg cgtattaccg caagggtttg gctctggtct ggtagcaatg
    22441 ccagacggcg ttttgcagac gcgtaccgac tttggtaaaa ccgggtacga tggcgcagca
    22501 ccgccgaaag gcgaaactca tcgctacatt tttaccgttc acgcgctgga tatagaacgt
    22561 attgatgtcg atgaaggtgc cagcggcgcg atggtcgggt ttaacgttca tttccactct
    22621 ctggcaagcg cctcgattac tgcgatgttt agttaatcac tctgccagat ggcgcaatgc
    22681 catctggtat cacttaaagg tattaaaaac aactttttgt ctttttacct tcccgtttcg
    22741 ctcaagttag tataaaaaag caggcttcaa cggattcatt tttctatttc atagcccgga
    22801 gcaacctgtg aacacatttt cagtttcccg tctggcgctg gcattggctt ttggcgtgac
    22861 gctgaccgcc tgtagctcaa caccacccga tcaacgtcct tctgatcaaa ccgcgcctgg
    22921 tacctcttct cgcccgattc tgtcggcaaa agaagcgcag aatttcgatg ctcaacacta
    22981 ttttgcatcc ctgacaccag gtgctgcagc gtggaatcct tccccgatta ccctgcctgc
    23041 gcaacctgac tttgttgtcg gcccggcggg tactcaaggt gtaacgcata ccacgattca
    23101 ggcggcggta gatgcggcaa ttatcaagcg cactaacaag cgccagtata ttgccgtgat
    23161 gcctggtgag tatcagggaa cggtgtatgt ccctgctgct ccgggtggaa ttactctgta
    23221 cggtacaggt gaaaaaccga ttgatgtgaa gattgggctt tcccttgatg ggggcatgag
    23281 ccctgccgac tggcgtcacg acgtcaaccc gcgcggcaaa tatatgccag gtaaaccagc
    23341 gtggtatatg tacgatagct gccagagcaa acgcagcgac agtatcggtg ttctctgctc
    23401 tgcggtcttc tggtcacaaa acaatggcct gcaactgcaa aatctgacca tcgaaaacac
    23461 gctgggcgat agcgtagatg caggtaacca tccggcggtg gcactgcgta ctgatggtga
    23521 ccaggtacag attaacaacg ttaacattct cggtcgtcag aacaccttct ttgtcaccaa
    23581 cagcggtgtg cagaaccgtc tggaaaccaa ccgtcagccg cgtacgctgg tgaccaacag
    23641 ctacattgaa ggggatgtgg atatcgtttc tggtcgcggc gcagtggtgt tcgataacac
    23701 cgaattccgc gtggtgaact cacgtactca gcaagaagcg tatgtgtttg caccggctac
    23761 gctgtccaac atttactacg gtttcctcgc cgtaaacagc cgtttcaatg ctttcggtga
    23821 tggtgtggcg caactgggcc gctcgctgga tgttgatgcc aataccaacg gtcaggtggt
    23881 gatccgtgat agcgccatca acgaaggttt taacacggct aaaccgtggg ccgatgcggt
    23941 gatctctaat cgtccgtttg cgggtaatac cggcagcgta gatgataacg acgaaataca
    24001 gcgcaatctg aatgacacta actacaaccg catgtgggaa tacaataacc gcggcgtggg
    24061 tagtaaagtg gttgcagagg cgaagaagta agagctatta actatttgcc ggatgcggcg
    24121 taaacgcctt atccggccta cagattcgat gcgattcgta ggtcggataa gatgcgcaag
    24181 catcgcatcc gacaataagt gccgaatgcg acctacattc acatggcgct ttttacatct
    24241 gacggttttt attgaagtta atcaaactac ccgccttgat aatctcgcgc tcttcagcag
    24301 tcagactttc catatagagc gtaatttccg ttaccggcgc atcttcatgg atcacataac
    24361 ctttaaacgt cgtacccgga ttatccagcg ccgctttaat gccagggatg taaatgtaat
    24421 cccccacttc aaaggttggc acttccgcca tctgcagcgg taacatcccc cagttgatga
    24481 cgttagaacg gtagcgttta gtcgcgtact cttcggcaat attcgccaga ccgccaatca
    24541 cacgctggca gctcgccgcc tgttcacgcg cagaaccatc gcctggtttc accgcatata
    24601 ccatgctgcc aatttcagtt tgcagcggat caatatgctc ctgaccagca atctgcttaa
    24661 tgcgcgcaaa cacctctgtc agctcgctga cattccccgc cagacgctga ttttccagct
    24721 cagcagtcgc tttacttctg ccaacataac cgggatcgcg gcgtgacagg gtaaactccg
    24781 ccagaccaat cggatttgaa cgataagaag aggtttcacc ggaaggaatc agttcgtcgg
    24841 tggtggtcac ttcgtcgagg atcttcgagc acactttcag gacgatattg tcagtcagcg
    24901 cacccaattc cggccagtct ttaatgttcg gtccgtaaat cagcggttgc tgagttgccc
    24961 ctttcacaaa gccctgataa acacggtttt tatacggcgt tacatcgaag gcgtactccg
    25021 gcacgttgtc ccagcaatca agttcgctgg cagaggttaa atagccaccg tttgccgcag
    25081 tcgcagcgat agaacgagcg tccatcaacg ccaccgctga catctgccca ttagctggct
    25141 tagagccttc gcggttcggg aagttacgcg tggtgtggcg aatacttaat ccgttgttga
    25201 ttggcgtatc gcccgcgcca aagcatgggc cgcagaacgc ggttctgatg attgcgcctg
    25261 cgccaatcaa atctgctacc acaccttttt tggcgagatc cataaacacc ggctgtgatg
    25321 acgggtaaac tgccagcgag aaggtgtcat tgccacagga ttgaccgcgc agtgcattcg
    25381 ccgccgcgat gacgttttcg tagttaccgc cagaacagcc cgcgataatc ccctgctgca
    25441 ctttcaggcg accattttcc actttatcca gcagcgagag tttggcttta ccgtgcgcca
    25501 cgcgttcgga ctcaatttca atctcacgca gaatgtcggt caggttctgg ttcagtgtgt
    25561 cgatttcata cacgttgctc gggtggaacg gcagcgcaat cattggtttg atggcgctta
    25621 aatcaacgct gatgcagcca tcgtagtacg ccatcggttg agggttaagc tggcagtaat
    25681 cctggccgcg accgtgcagc gccagccagt tatgcacttc ttcatcagtt tgccagacgg
    25741 agcttaaaca ggtggtttcc gtggtcatca cgtcaacgct gttacggaaa tcggtagaga
    25801 gcgcgctaac gcccggtcca acgaactcca tgactttgtt tttgacgtaa ccgtttttga
    25861 acaccgcgcc aatgatagcc agcgccacat cctgcggccc cacatacggc gctggttttc
    25921 cggtcagatg caccgcaaca actcccggat agtcgatatc ccaggtgtca ttaagcagct
    25981 gttttaccaa ctcaccaccg ccctcaccga ctgccattgt ccctaatgca ccgtaacggg
    26041 tgtggctgtc tgacccgagg atcattttgc cgccgcctgc catcatctca cgcatatatt
    26101 gatggatgac cgcaatatgc ggaggcacaa aaataccgcc ataacgctga gctgccgata
    26161 aaccaaaaac atggtcatca ccgttaatgg tgccgccgac ggcgcagagt gagttatggc
    26221 agttggtcag cacatacggc agcgggaaac gttccatacc ggacgcttta gcggtctgta
    26281 caataccaac aaaggtaata tcgtgagagg ctaatgaatc aaacttaatt ttaagtttat
    26341 ccatatttcc ggacgtatta tgcgaagaga gaatagacca ggcaatagtg ccttttttgg
    26401 cttcttcttt tttaatttcg ccggtgaaat gttcttcagc aattatttcg ttattactgg
    26461 cgagaaacac gcctttttca gataacttga tcatcaaatg ctccagatat ctgcgcaccg
    26521 tgaagtgcgc agaaagataa taaatggata tactctaaat aatttgagtt gcaggaaggc
    26581 gacaagcgag tgaatcccca ggagcttaca taagtaagtg actggggtga gcgaacgcag
    26641 acgcagcaca tgcaacttga agtatgacga gaatatttac agcatgttcc agccgatcag
    26701 catattccac caccacacgc cgagggtgat atgcaccagg aaggttaata tcgtcagtac
    26761 cgcaccgacc aaccaccagg atttaatatc gttataaccc acgccaaaga tgaccggacc
    26821 tgccgcgccg ccatagtgag taaccatgcc gccataggag ttggagaaca acagtgccag
    26881 cgcggttaac attaacggtg cgccggagac gttcgccagc atggcaaata ccggtaacat
    26941 agcaacgata taggcactac cggaagcgaa gaaataacgc acgataatgc tgaggaaaat
    27001 aataacgaag aaagcaacgt taccgtgacc atcaaatgcc aggttatttt taaagacttc
    27061 agctaaccat tcgaagaatt taactttcga taataaggag cttaatccga taataccgcc
    27121 gtaccagatt aaggtattcc agccgccttt atttttaacc acgtcttccc aggtaacgat
    27181 acccagcagc agcatggtcg ccataacaac gattgccaca gtggattcat caacccccag
    27241 agacttactg aaaatccagc ccagtagcgc cagcacaaag acaccgagca gcattttttc
    27301 gcggattttc atcggtccca gttcggcaag gcccgcttta gcgatggttt tgttatccac
    27361 ctttttaatt tctggtggat acatggtgta aattaccagc ggggtgacca gcagcataat
    27421 gatgcccggc aatccggcgg ccagcgccca tccaccccag ctaatttgca ggtgcagaat
    27481 gtcgttgatc attttcagcg ccagaatgtt ccccgccatt gcggtaaaga acatatagct
    27541 ggtggttttg gtgaccatgt aaatggacat catcaggtaa tgtccgacac gacgcggact
    27601 tttttccggt tcggacccca aagccaccgc cacgctgttg atgatcggta acacaatgcc
    27661 gcccgcacgc gcggtgttag acggtgttgc cggagccagt accagatcga ggaataccgt
    27721 aacgtaaccc agacccagcg tggtgttacc gattttacca atcagcagat aggcaatacg
    27781 tttacctaaa ccggtggtca caaatgcggc gcttaaggta aacgccgaga acaccagcca
    27841 ggtagtacct gaagagtaac cgcttaatac ggcggtggtc ttaaacgcac cgtcggataa
    27901 gttaccgacc accaccattg aggcagcaac ggcaattaac agtacgacag gttccgggaa
    27961 aggcttgata accagaccca caatggccgc caggtaaata ccaaaaagca cccacgccag
    28021 ttcgcttaat cctgccggag ctggcataaa accaataata catgggatcg ctaatatcag
    28081 aattagcttc cataacgatt tcttattcat ttaaaatcct gttctttctg tatgactcat
    28141 acagaagtcc tttattgatt gtccttatcc tgacaacgaa ttttttcagg gaagataaac
    28201 ttcaccggaa aatatttttc tggtcgtccg aataacagat gcgcgtaacg tcgtggcatc
    28261 ctgaccttca ttacttaaat gaacgtcgag cgcaccactg gggtgttcaa tattgatatt
    28321 gccgtatcct acagaaggga cgatttgtcg ggtgacggtg ccttccaatg cacaactact
    28381 ggaaatagca atagcaccgg ttatcgccag cgcgcgatgg ca
//
LOCUS       DAFATA010000052        28391 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFATA010000052 DAFATA010000000
VERSION     DAFATA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.053"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..790)
                     /locus_tag="LGJ20_002725"
     CDS             complement(<1..790)
                     /locus_tag="LGJ20_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK2386761.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRE"
     gene            complement(808..2724)
                     /gene="mngA"
                     /locus_tag="LGJ20_002726"
     CDS             complement(808..2724)
                     /gene="mngA"
                     /locus_tag="LGJ20_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBK2386762.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2893..3615
                     /gene="mngR"
                     /locus_tag="LGJ20_002727"
     CDS             2893..3615
                     /gene="mngR"
                     /locus_tag="LGJ20_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBK2386763.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQAKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(3719..4588)
                     /gene="sucD"
                     /locus_tag="LGJ20_002728"
     CDS             complement(3719..4588)
                     /gene="sucD"
                     /locus_tag="LGJ20_002728"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBK2386764.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(4588..5754)
                     /gene="sucC"
                     /locus_tag="LGJ20_002729"
     CDS             complement(4588..5754)
                     /gene="sucC"
                     /locus_tag="LGJ20_002729"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBK2386765.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(5848..7065)
                     /gene="odhB"
                     /locus_tag="LGJ20_002730"
     CDS             complement(5848..7065)
                     /gene="odhB"
                     /locus_tag="LGJ20_002730"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBK2386766.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(7080..9881)
                     /gene="sucA"
                     /locus_tag="LGJ20_002731"
     CDS             complement(7080..9881)
                     /gene="sucA"
                     /locus_tag="LGJ20_002731"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBK2386767.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(10082..10798)
                     /gene="sdhB"
                     /locus_tag="LGJ20_002732"
     CDS             complement(10082..10798)
                     /gene="sdhB"
                     /locus_tag="LGJ20_002732"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBK2386768.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(10814..12580)
                     /gene="sdhA"
                     /locus_tag="LGJ20_002733"
     CDS             complement(10814..12580)
                     /gene="sdhA"
                     /locus_tag="LGJ20_002733"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBK2386769.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(12580..12927)
                     /gene="sdhD"
                     /locus_tag="LGJ20_002734"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(12580..12927)
                     /gene="sdhD"
                     /locus_tag="LGJ20_002734"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBK2386770.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(12921..13325)
                     /gene="sdhC"
                     /locus_tag="LGJ20_002735"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(12921..13325)
                     /gene="sdhC"
                     /locus_tag="LGJ20_002735"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBK2386771.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            13706..13813
                     /locus_tag="LGJ20_002736"
     CDS             13706..13813
                     /locus_tag="LGJ20_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386772.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            14019..15302
                     /gene="gltA"
                     /locus_tag="LGJ20_002737"
     CDS             14019..15302
                     /gene="gltA"
                     /locus_tag="LGJ20_002737"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBK2386773.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            15693..16259
                     /locus_tag="LGJ20_002738"
     CDS             15693..16259
                     /locus_tag="LGJ20_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2386774.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            16310..18769
                     /locus_tag="LGJ20_002739"
     CDS             16310..18769
                     /locus_tag="LGJ20_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2386775.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            18784..19515
                     /locus_tag="LGJ20_002740"
     CDS             18784..19515
                     /locus_tag="LGJ20_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2386776.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            19512..20573
                     /locus_tag="LGJ20_002741"
     CDS             19512..20573
                     /locus_tag="LGJ20_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2386777.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            20725..21771
                     /locus_tag="LGJ20_002742"
     CDS             20725..21771
                     /locus_tag="LGJ20_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK2386778.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(21768..22559)
                     /gene="nei"
                     /locus_tag="LGJ20_002743"
     CDS             complement(21768..22559)
                     /gene="nei"
                     /locus_tag="LGJ20_002743"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBK2386779.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(22595..23329)
                     /gene="pxpA"
                     /locus_tag="LGJ20_002744"
     CDS             complement(22595..23329)
                     /gene="pxpA"
                     /locus_tag="LGJ20_002744"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBK2386780.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(23319..24251)
                     /gene="pxpC"
                     /locus_tag="LGJ20_002745"
     CDS             complement(23319..24251)
                     /gene="pxpC"
                     /locus_tag="LGJ20_002745"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBK2386781.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(24245..24901)
                     /gene="pxpB"
                     /locus_tag="LGJ20_002746"
     CDS             complement(24245..24901)
                     /gene="pxpB"
                     /locus_tag="LGJ20_002746"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBK2386782.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(24924..25667)
                     /gene="ybgI"
                     /locus_tag="LGJ20_002747"
     CDS             complement(24924..25667)
                     /gene="ybgI"
                     /locus_tag="LGJ20_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBK2386783.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            25938..27419
                     /gene="dtpD"
                     /locus_tag="LGJ20_002748"
     CDS             25938..27419
                     /gene="dtpD"
                     /locus_tag="LGJ20_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBK2386784.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(27461..>28391)
                     /gene="phrB"
                     /locus_tag="LGJ20_002749"
     CDS             complement(27461..>28391)
                     /gene="phrB"
                     /locus_tag="LGJ20_002749"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBK2386785.1"
                     /translation="EGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKA
                     AIAQLRQFCQNGAGDYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALD
                     GGAGSVWLSELIWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTG
                     YPIVDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNG
                     GWQWAASTGTDAAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQ
                     KAGVKLDYPQPIVDHKEARLRTLAAYEEARKGA"
BASE COUNT         6788 a         7706 c         6999 g         6898 t
ORIGIN      
        1 tttcacgctg cgcttcaatc tgctcgaata cctcttcaaa gcggctcatc acaaactttc
       61 gttgaggata aatttcgcga agtttctcca tcacttcgaa gatattttgc tgcaatggca
      121 tctggtcatg accattcggc aataaaatct ctttggttac agacgctttt tccagcacgt
      181 caaaataact gtcgaggcgt ttgcgtaatc cgttttcgtc ggcaggtaag tacttaccga
      241 tggcataacc caacggcagc acctgcgccg tcacttcgct accgtcactg ctttgccata
      301 aaaactcggt tttatcagta ccgtggcgct ccgaacatcc gcgccagaac atagtgcggg
      361 taatgccaaa tccattgtag atatgcggca gttgcccgga catgccaaag gaatccggta
      421 aataacctat tttcatcggc tcgccaaacg cgaggcagtc acgcattccg tacatcagat
      481 tacggacgat ggattccgca gaaacaatcg tggtatcggt ctgggtatac caggggccga
      541 taatcaactt accggcttct acctgtttct tcacgcggtc tttgttttcc ggtttcaccg
      601 cgaaataatc ttcgaggatc gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt
      661 cctgttccag tcggcacagg atctcttcca tattattgac cagcagaatg cgtgactctt
      721 cggtggtgaa ataccactcc cgatcccaat gcatatgcgg ggtgatgtga acgcgagata
      781 ccgctttcat cgtcgtttcc tttttgttta tggcattacg ccatcagtca gatagttgcc
      841 atgcttaacc gcgtgacgcc gccagatcag gagaattgca gtcgagattg cagcccccac
      901 caatgccgca ccaaaccagc caattgccgc cataacaccg cccgcgccat tatcatgaag
      961 taaaaagagc gagaaaatgc cggcaccggg tgtcgaaagg ccgatattca tcgcaccgac
     1021 aatagcgccc gttaccatag agcccagcac aaacgaacca ataacccgca gcggatcttc
     1081 aatcgccatc gggatcgccc cttcggtaat acctgccagc cctaacagcc aggtggattt
     1141 cccggtctca atttcaaact ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac
     1201 ggtaaatgcc gaaaccattt tgacggaggc gaaaatggca tacgggccgt aaacgccgtt
     1261 cgccattgcg cccaggcaga atgcataagc ggctttgttc actggccctc caaggtcaaa
     1321 ggaacacata aaaccgagaa tggcacccag caacagcgcg ttacttcctg acagaccgtt
     1381 cagccaggcg gtaagcgagt tattgatcca ggcgacaggt tctcccacca caaacagcat
     1441 cagactgccc gctcccaacg taccgagcac cgggtagaga taaaaagtca ggaatccatt
     1501 gaatttactg ctaagacgca agtgattttt cacccagcgc atcaagtaac cggctatcaa
     1561 tccgccaacg accgcgccga gaaacccgga gccgatcatg ttggcggcaa gtccagccgc
     1621 aaagcctggc gctaacgccg gtttatctgc cagagaatag gcggtatagg ccgcgagcac
     1681 cggtaccatc aaaattccga gcagcccgcc gcccagcttg cggtacatcc acagccagga
     1741 gttttcttca ttaaacagat cttgtagccc gaagatttgc gacagtaata ccgcgaccgc
     1801 cagcaccgtg ccccccgcga caatcaacgg tacggcaaaa gagattccgc tcaacagtgc
     1861 ctgtttcagc tccgttttga cacttttcac cggttgcgta tcttgctgta cggtacgcgt
     1921 ctcatcgcta cgcttgaggg taagcgcttg ttggatcaac gcttctgcat ggcgaatcgg
     1981 ctcggcaaca ggcactgaaa gcgcgggaat gccattaaaa cgctcactct ccttgatggc
     2041 gacttcagcc gcaaaaatac aggcggttgc actattgagt tgatccgccg ttaaacgccc
     2101 ttcaatgccg ttagcgcctt gtttttcaac gtaaacattt acgccgagtt tgcgtccggc
     2161 tttttccaga tattccgcag ccatataggt gtgagcaata cccgccggac aggccgttac
     2221 gcagacgata gttggcgcgt tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc
     2281 cagcgccgag agcaactcat caggcgtcgt cgccgactgt atacgcgccc gaatctcatc
     2341 atccgccagg cgcgtggtca gcgctgtcag cagttgcata tgcgtagtac ccgcttcatt
     2401 ggggggaatc gccagcagca ccactaaatc aaccgcttcc gggccatcaa cgccttccca
     2461 ctgaagcggc tcgctgagtg tggcgaccgc aaacgccgct tcttttaccg cagcagtttt
     2521 gccatgcggc acagccaacc cttcacctaa ggcggtcggg ccaaggcttt cacggcgata
     2581 cacttcttcc agaaattgct cagtactgga aattttcccc agagcagcaa gacgttgagt
     2641 caacgcgtgg atggcctctt cacggctggt aaagcgcgca ttcagacaca acgcatcgcg
     2701 gtgggtcaga gtcgtcagat tcatcatgat cctcacttga tcgtttttat agtcgcgcca
     2761 gtgtgcccga taaaacaata ccattatgtg atcaatttca cttgattgaa attatttgta
     2821 ttgtatttgt attatttaca ggatgaattt atcgggcata atggctaaat tcgtattaat
     2881 gagatactaa aaatgggaca caagccctta taccggcaga ttgccgatcg cattcgtgaa
     2941 caaattgcgc gtggcgagct taaacctggc gatgcgttac ctaccgaatc ggcattacaa
     3001 acagagtttg gcgtcagccg ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa
     3061 cagatcctcg aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac
     3121 gatatttttc agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac
     3181 agtgaagtcc tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa
     3241 atcaccgcgc aggatcgtgt gtggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca
     3301 atggcgctgg aagaaacctg gatgccgctt gcgttgttcc cggatctcac ctggcaggtc
     3361 atggaaaatt caaaatatca ctttattgaa gaagtgaaga aaatggttat cgatcgtagc
     3421 gaacaggaaa tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc
     3481 caggcaaagc cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt
     3541 gaatatagcc gcaacgcttt taataccgat gactataaat ttaccctgat agcgcaacga
     3601 aaatcatcgc gataagcaca aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga
     3661 cagcccttcg ccattcctgt agagggaagc ggcgagggct atttcttatt acagatattt
     3721 atttcagaac agttttcagt gcttcaccga tatccgccag gctgcgaacg gttttcacgc
     3781 ctgcggcttc cagagcagca aatttctcat ccgcagtccc tttcccaccg gcaatgatgg
     3841 cacccgcgtg gcccatacgt ttgcctttcg gcgcagtcac accagcgatg taacccacaa
     3901 ctggcttggt aacatgctct ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga
     3961 tctcaccgat cattacgatc gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt
     4021 cgataaagtt agagccaggg atcgggtcac cgccgatacc aacacaggtc gactgaccga
     4081 aaccgtaatc cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa cgggaaacga
     4141 tacccacttt acccggtttg tgaatgtgac ccggctggat accgattttg cattcacccg
     4201 gagtgataac gcccgggcag ttcgggccga tcatacgaac gccggcttcg tccagtttca
     4261 ctttcacagt cagcatatcc agcgtcggga tgccttcagt gatggtgata atcagtttga
     4321 tgcctgcatc aatagcttcc agaatggagt ctttgcagaa cggtgctggt acgtagataa
     4381 cagaagcggt agcgccagta gcagcaacag cttcacgcac ggtgttgaac accggcaggc
     4441 cgaggtgggt agtaccgcct ttacctgggg ttacgccgcc aaccatttta gtgccgtatg
     4501 caatggcctg ttctgagtgg aaagtcccct ggctaccagt aaagccctgg cagataacct
     4561 tggtgttttt atcaattaaa atggacatta tttcccctcc actgcggcaa caacctgctg
     4621 agctgcatcc gtcagacctt ttgctgcaat aatattcagg ccgctgtcag ccagtttctt
     4681 cgcgccgagt tcggcgttgt taccttccag acgtaccacg accggtacgt taacacccac
     4741 ttctgctacc gcgccgatga taccgtcagc gatcaggtcg caacgaacga taccgccgaa
     4801 gatgttaacc agaacggctt tcactttgtc gtcagagagg atgattttga acgcttcggt
     4861 tacacgttct ttggttgcgc cgccgccaac gtcaaggaag ttagccggtt cgccgccgtg
     4921 cagtttaacg atgtccatcg tacccatcgc cagacctgcg ccgttaacca tacaaccgat
     4981 gttaccgtcc agcgcaacgt agttcagttc ccactgtgca gcctgtgctt cacgcggatc
     5041 ttcctgcgac tggtcacgca tttcgcgcag atcaggctgg cggaacagtg cgttgccgtc
     5101 agcgcccagt ttgccgtcga ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt
     5161 gatttcgatc aacgccaggt cgcgctccag gaaaatggtc gccaggccca tgaagatttt
     5221 ggtgaactgc tgaaccagtt taccttccag acccagtttg aacgccagct cgcgtccctg
     5281 atacggcatc gggcctgtca gcggatcaag cgcaacttta tggatcaggt gcggagtttc
     5341 ttccgccact ttttcgattt ccacgccgcc ttcggtggag gccataaaga ccacgcgacg
     5401 ggaactacgg tctacaacgg caccgagata cagctcttta gcgatatcgg tcgctgcttc
     5461 aaccagaatc tggttaaccg gttggccatt ggcatctgtt tgatacgtta ccagacgctt
     5521 gccgagccag ttttctgcaa aagcacggat gtcttctttg ctgtttacaa ctttcacacc
     5581 gcccgcttta ccgcggccac cagcgtgaac ctgacatttc actacccacg ggccggcacc
     5641 gatttttgaa gcggcttctt ctgcttcgcg cggagtagta caggcataac ccaccggtgc
     5701 tggtaagcca tagcgggcaa aaagttgttt tgcctgatat tcatgtaagt tcatgtgttc
     5761 tgtccatcct tcagtaatcg ttatctttta aaccgtagac cgacacccta tccacgtcgg
     5821 tctacagtgc aggtgaagct taaactacta cacgtccagc agcagacgcg tcggatcttc
     5881 cagcaactct ttgatcgtta ccaggaagcc cacggattcg cgaccatcga tcagacggtg
     5941 atcgtaggac agcgccaggt acatcatcgg caggatctca acctgaccat tcaccgccat
     6001 cggacgatct ttgatagcgt gcatacccag aattgcgctc tgcggcgggt tgatgatcgg
     6061 cgtagacatc agggaaccga acacaccacc gttggtgatg gtgaagttac caccggtcag
     6121 atcttcaacg gtcagcttgc cgtcacggcc tttgactgcc agctctttga ttttcttctc
     6181 gatgtctgcc atgccgaggg tatcgacatc acgcagtacc ggtgtcacca ggccgcgcgg
     6241 cgtagaaacc gccatgctga cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat
     6301 agacgcgttc acttccgggt aacgtttcag ggcttcaacc accgctttca cgtagaagga
     6361 cataaagccc agacggatgc cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag
     6421 atccataatc ggcttcatgt tgacttcgtt gaacgtggtc agcatggcgg tggagttttt
     6481 cgcttccagc agacgctctg ccacacgctt acgcaggcga gtcatcggga cacgtttttc
     6541 actacgtgca gccagagccg gttgcgccgc cggatcagcc gctgccggag cagactcttt
     6601 cgccggggct ttcgccagat gtttttccac atcttcacga gtcagacgac cacccacacc
     6661 ggtgccttta atggcgctgg cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg
     6721 gcttaacgca tcgttgtttt gctcttccag agacgcctgc tggcgttgcg ccggagtgga
     6781 cgctttctct tcagatttgg cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg
     6841 accaaggatc tgacgagacg ttaccgttgt accttcatct tccagaaccg catccagaat
     6901 gccgtctgct gatgccggta cttccagtac cactttgtca gtttcgattt ctaccagcac
     6961 ttcatcacgt acgactgcgt cgccgggttt tttatgccag gttgcgacgg tggcatcggc
     7021 tacggattca ggcaggtcag ggaccagaat atctacgcta ctcattgtgt atcctttatt
     7081 tattcgacgt tcagcgcgtc attaaccaga tcttgttgct gtttctggtg aacggacata
     7141 taccctaccg ccggagaggc ggaggccggg cggcctgcat aacgcagaga agccccaaac
     7201 ggaatcactt cacggaaatg atgctggctg cagtaccatg cgccctggtt gagcggctct
     7261 tcctggcacc agacaaaatc cttgacgtga gcaaactgct gcaacacttc ctgcatcgct
     7321 ttatgcggga acgggtagag ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc
     7381 ttacgacgct gttccagcag gtcgtaataa accttaccag aacacattac tacgcgcttc
     7441 acgcccttcg gatcaagctc gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc
     7501 agttcttcga ggctggaaac cgccagcgga tgacgcagca gggatttcgg cgacatcacg
     7561 accagcggac gacgcatccc gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc
     7621 ggggtagacg gtacgcaaac ctgcatgttt tgctcagcac aaagttgcag ataacgttcc
     7681 agacgcgcgg aggagtgctc cggcccctgc ccttcgtaac cgtgcggcag caacatcacc
     7741 agaccacaca tccggcccca tttctgctcg ccagaggaga tgaactggtc gataaccacc
     7801 tgcgcaccgt tggcgaagtc accgaactgc gcttcccaga tggtcagggt gcgtggttct
     7861 gcggtggcat aaccgtattc aaacgccagc actgcttctt cagacagtac ggagtcccaa
     7921 acacggaacg cgccctggcc gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta
     7981 gactggttgt ggatcaccgc gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc
     8041 gacaggcgaa ccggaatgcc ttcgtcaacc agcgtggcgt aagcgaggtt ttccgcgccg
     8101 ccccagtcga acagtttctc accggcagcc atcgcctggc gatcgccata aatcttggca
     8161 acgcgagact gcatttcaac cgcttccggc accgtgctga tgcgtttagc cagttcctgc
     8221 aggcgcttca tctcaacttt gttcgggtac tcttcgtccc attcgtggtt gaggtacggc
     8281 gaccaggtga aagagtgcat gttcatcgga cgccactctg ctaccacgca atcgccagca
     8341 tccagcgcat cgcggtacag gttaaccatc tcggtggcat cttccagcgt cgccactttt
     8401 tcctgctcca gcttgtcagc gtagattttg cgcggtgtcg gatgtttttt gattttctga
     8461 tacatcagcg gctgggttgc gctcggctcg tcggcttcgt tgtggccgtg acggcggtag
     8521 cataccaggt cgatgaagac atcacgttta aaggtgttac ggaaatcgag cgccagacgg
     8581 gtcacaaagg caacggcttc cggatcgtcc gcgttaacgt ggaaaatcgg tgcctgaacc
     8641 atcttgccga tatcagtaca gtacggcgta gaacgggcat ccagcggatt agaggtggtg
     8701 aaaccaacct ggttgttgat aacgatacgt accgtaccgc caacttcata accacgcgct
     8761 ttcgacatgt tcagggtttc ctgaaccacg ccctgtccgg tcactgcggc gtcaccgtgg
     8821 atggtaattg gcagcacttt gttgctgctc ggctcatcaa gtctgtccag acgggcacga
     8881 acagagccga taacgaccgg gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc
     8941 aggtgcacca ggccgccatc ggtctggaag tcagacgaga agcccatgtg gtatttcacg
     9001 tcacccgtgc cgaggtgttc tttatgttta ccggcaaact cgtcgaacaa gtcttgcggt
     9061 tttttaccca gcacgttcac caacacgttc agacgaccac ggtgcgccat cccgagaacc
     9121 acttcccggg tgccgctgtt gccagcgtgg cggatcatct ctttaagcat cgggattaac
     9181 gcgtcaccgc cttccagcga gaagcgtttt gcgccaggga attttgcgcc gaggtaacgt
     9241 tcaaggcctt cagcggcggt cagttcgctt aagaagcgtt ttttctcttc gctattgaaa
     9301 gtcgcgcgac cagactcgat acgctgttgg atccagcgtt tttcttcggt gctggtaatg
     9361 tgcatatact cggcaccaat cgggccgcag taggtttgct tgagggcttc cagcagctcg
     9421 ccgagtttca tggtttcttt gccgctggca aatgaaccga cgttgaaggt ctcctggaag
     9481 tctgcttcgg tcagatcgtg gaaagacgga tccagatcgg ccactttatc ttgctgccac
     9541 agtcccagcg gatcgagatt cgcatgctgg tgaccacgga agcggtatgc gttaatgagc
     9601 tgcaggactt taacctgctt tacattggtg tcagggtcgg agatcgttga agagtaacgt
     9661 gaagcgtctt tcgccaggcg gcggaaatat tcacgcgttt gagagtggaa ttgatccggt
     9721 ttgactcccg taccaggtaa ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca
     9781 ggatcggtta agaagtcttc atagagctgt tctatccagc tctggtttgc gccagagagg
     9841 taagaagagt ccaaccaggc tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct
     9901 tttttatgct tacttcgccg tggatactac cacgcacagt tatgtgcgtg ccggggttca
     9961 cttgcgagct cttgtctacg tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt
    10021 caggggcagt ttttaaagat ttcctgaata gccgggggat ttccccggca tcaacattta
    10081 attacgcatt acgttgcaac aacatcgact tgatatggcc gatggcgcgc gtcgggttca
    10141 gccccttcgg acatacactg acgcagttca tgatgctgtg acagcggaat acactgaatg
    10201 catcgctcaa accgtcgagg cggctgtcag tctcggtatc acggctatcg atcaggaaac
    10261 gatatgccgc taacaagcct gccgggccga taaacttatc gggattccac cagaaagacg
    10321 gacaagaggt tgaacaacat gcgcagagaa tacattcata cagcccgtcg agtttttcgc
    10381 gctgctctgg catctgtaaa tgctcgcgag ctggcggatt ttgtccatta ttcaacaggt
    10441 aaggcttaat tttctcatat tgcgcataga attgtcccat gtctaccacc aaatcgcgga
    10501 tcaccggtaa acctggcagc gggcgaatca caatcttctt gcccggctgg ttgagtgccg
    10561 aaatcggggt aatacaggcc agaccattct tgccgttcat gttcagaccg tcggaaccgc
    10621 acacaccttc acggcaggag cggcggaacg acaggctggg atctttctct ttcagctgga
    10681 taagcgcatc cagcaacatc atgtcgcgac cttcttccgc ttccagggtg taatcctgca
    10741 tacgcggagc atcatcaaca tccgggttat agcgataaat tgaaaactcg agtctcattt
    10801 tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa tgccgggcgc agtttcggtt
    10861 ccatgttgac gcttcggcgc gtcatggatt ccgactctgg cagatacagg gagtggcaca
    10921 gccagttttc atcatcacga tccgggaagt cgaagcggct atgcgcgcca cggctttcgg
    10981 tacggaagtt ggcagaaaca gccgttgcat acgccgtttc catcaggtta tccagttcca
    11041 ggcactcaac gcgctgggtg ttgaactcgc tggaagtgtc atccagacgg gcatttttca
    11101 gacgctcgcg gataactttc aactgctcaa gccctttcgc catcgcatca ccttcacgga
    11161 agaccgagaa gttatgctgc atacattctt gcagcgcttt acgaatcgcc accggatctt
    11221 caccgttacg attattgttc cagcggttca gacgatccag agatgcttca acatcagact
    11281 cgctggcatc gcgcaatgcg ccctgctcgg cgatagactc ttgcagatgc agacccgctg
    11341 cgcgaccaaa gaccaccagg tccagcagcg agttgccgcc cagacggtta gcgccgtgta
    11401 ccgatacaca agcgatttca ccaacggcaa acagccccgg aacaaccaca tcttcgcctt
    11461 tctcgttcac cgtcagcgcc tgaccggtca ctttggtcgg aataccaccc atcatgtagt
    11521 gacaggttgg gataaccgga atcggctctt tcaccggatc aacgtgagcg aaggtacggg
    11581 agagttccag aatacccggc agacgggatt cgagaacttc tttacccagg tggtcgagtt
    11641 tcagtttcgc atgtggcccc cacggaccat cacagccgcg accttcacgg atttcaatca
    11701 tgatggaacg cgccaccacg tcacggcccg ccaggtcttt ggcgttcggc gcataacgct
    11761 ccataaagcg ttcgccatgt ttgttcagca gataaccgcc ttcaccacgg caaccttcgg
    11821 tgaccagtac gcccgcaccg gcaataccgg tcgggtggaa ctgccacatt tccatatcct
    11881 gcaccggtac gcctgcgcgg atagccatgc cgacgccgtc gccagtgtta atgtgggcgt
    11941 tagtggtgga ctgataaata cgccctgccc cgccagtcgc cagcacggta gcgcgggctt
    12001 tgaaataaac cacttcaccg gtttcgatgc acagtgcggt acaacccacc accgcgccat
    12061 cctggttttt caccagatcc agcgcatacc actcggagaa aatggtggtg tggtttttca
    12121 ggttctgctg ataaagcgtg tgcaacagtg cgtgaccggt acggtcagcc gccgccgcag
    12181 tgcgtgccgc ctgctcgccg ccgaagtttt tcgactgacc gccaaacgga cgttgataga
    12241 tacgaccatc atcaagacgc gagaacggca ggcccatgtg ttcgagttcc agaatcgctt
    12301 ccggcccggt tttacacata tattcaatcg cgtcctggtc accgatatag tccgaccctt
    12361 ttacggtgtc gtacatatgc cattcccagt tatcttcatg ggtattaccc agcgcaacgg
    12421 taatgccgcc ttgcgcagaa acggtatggg aacgggtcgg gaagacttta gagagcagcg
    12481 cacaggtctg gccgctctgg gaaatttgca gcgccgcgcg catacctgcg ccaccggcac
    12541 caatcacaac tgcatcaaat tctctgactg gcaatttcat cacacacccc acaccacaac
    12601 gaatccataa atcacgtaaa ccaccagtgc aacgacaatc accagttgca gcatcaggcg
    12661 caatgccagc ggtttaacgt agtcggtcaa cacctgccac atgccgatcc aggcatgaat
    12721 caagatagaa aacagcgcca gcagggtgaa cactttggtg aacgcagagg cgaagaaacc
    12781 gatccagact tcatatgtca gttcgccact ggtggcgaaa aaaccgacca tataaatgat
    12841 gtagagcgtc aggacgatag cggtagcgcg aacgaggatg aaatcatgta cgccattgcg
    12901 tcctaatgcg gaggcgttgc ttaccatacg aggactcctg cgagaagtga aagcacgaca
    12961 gtaataacaa aggagatttt ggcggagcgt ttacccgctt cgaatgtttc ttccagatag
    13021 ccaaaatcca tcatcatgtg gcgaatacct acgacgacgt gatacgccag agcggtaagg
    13081 atgccccaca tgataaattt gacgaagaag ctgcccataa tcgcggaagc ttgctcgaaa
    13141 ccttcagggg aagagaggct ggtacccaga agccacagca ggatgcccac tgcaacaaag
    13201 gtgatcacac cggaaacgcg atggagaatg gacgctatcg ccgtgatggg gaaccggatg
    13261 gtctgtaggt ccagattaac aggtctttgt tttttcacat ttcttatcat gaataacgcc
    13321 cacatgctgt tcttattatt ccctggggac tacgggcaca gaggttaact ttctgttacc
    13381 tggagacgtc gggatttcct tcctccggtc tgcttgcggg tcagacagcg tcctttctat
    13441 aactgcgcgt catgcaaaac actgcttcca gatgcgaaaa cgacacgtta caacgctggg
    13501 tggctcggga ttgcagggtg ttccggagac ctggcggcag tataggctgt tcacaaaatc
    13561 attacaatta acctacatat agtttgtcgg gttttatcct gaacagtgat ccaggtcacg
    13621 ataacaacat ttatttaatt tttaatcatc taatttgaca atcattcaac aaagttgtta
    13681 caaacattac caggaaaagc atataatgcg taaaagttat gaagtcggta tttcacctaa
    13741 gattaactta tgtaacagtg tggaagtatt gaccaattca ttcgggacag ttattagtgg
    13801 tagacaagtt taataattcg gattgctaag tacttgattc gccatttatt cgtcatcaat
    13861 ggatccttta cctgcaagcg cccagagctc tgtacccagg ttttcccctc tttcacagag
    13921 cggcgagcca aataacaaac gggtaaagcc aggttgatgt gcgaaggcaa atttaagttc
    13981 cggcagtctt acgtaataag gcgctaagga gaccttaaat ggctgataca aaagcaaaac
    14041 tcaccctcaa cggggacaca gctgttgaac tggatgtgct gaaaggcacg ctgggtcaag
    14101 atgttattga tatccgtact ctcggttcaa aaggtgtgtt cacctttgac ccaggcttca
    14161 cttcaaccgc atcctgcgaa tctaaaatta cttttattga tggtgatgaa ggtattttgc
    14221 tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc taactacctg gaagtttgtt
    14281 acatcctgct gaatggtgaa aaaccgactc aggaacagta tgacgaattt aaaactacgg
    14341 tgacccgtca taccatgatc cacgagcaga ttacccgtct gttccacgct ttccgtcgcg
    14401 actcacatcc aatggcagtc atgtgtggta ttaccggcgc gctggcggcg ttctatcacg
    14461 actcgctgga tgttaacaat cctcgtcatc gtgaaattgc cgcgttccgc ctgctgtcga
    14521 aaatgccgac catggccgcg atgtgttaca agtattccat tggtcagcca tttgtttatc
    14581 cgcgcaacga tctctcctat gccggtaact tcctgaatat gatgttctcc acgccgtgcg
    14641 aaccgtatga agttaatccg attctggaac gtgctatgga ccgtattctg atcctgcacg
    14701 ctgaccatga acagaacgcc tctacctcca ccgtgcgtac cgctggctct tcgggtgcga
    14761 acccgtttgc ctgtatcgca gcaggtattg cttcactgtg gggacctgcg cacggtggtg
    14821 ctaacgaagc ggcgctgaaa atgctggaag aaattagctc cgttaaacac attccggaat
    14881 ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct gatgggcttc ggtcaccgcg
    14941 tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga aacctgccat gaagttctga
    15001 aagagctggg caccaaagat gacctgctgg aagtggctat ggagctggaa aacatcgcgc
    15061 tgaacgaccc gtactttatc gagaagaaac tgtacccgaa cgtcgatttc tactctggta
    15121 tcatcctgaa agcgatgggt attccgtctt ccatgttcac cgtcattttc gcaatggcac
    15181 gtaccgttgg ctggatcgcc cactggagcg aaatgcacag tgacggtatg aagattgccc
    15241 gtccgcgtca gctgtataca ggatatgaaa aacgcgactt taaaagcgat atcaagcgtt
    15301 aatggttgat tgctaagttg taaatatttt aacccgccgt tcaaatggcg ggttaatttt
    15361 tatatgccta aacacaaaaa attgtaaaaa taaaatccac tatcggacat tcatatattc
    15421 taaaaaaaaa tatatcggta taaattataa acaaatcgat gatttttaat catctatctt
    15481 cctctcagtt acatgaataa accaccaaat atagcgcacc cctataattt ccaatacgtt
    15541 attcatcaaa catttaattc ataccatcaa ttaattactt ttacatttaa taaaattaat
    15601 aatattttta agaaaaacta aaaacattcg acaatcaaat aagatattct tgcaccgata
    15661 tacttttttc ttttctgtta aaggactccg ttatgtttaa aggacaaaaa acattggccg
    15721 cactggccgt atctctgctg ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg
    15781 aaattcactt taaaggtgaa gttattgaag caccgtgtga aatacatcag gatgatattg
    15841 ataaagaggt tgaactcggt caggtgacca ccagccacat taatcagtca catcacagcg
    15901 atgccgttgc tgtcgacctg cgcttagtca actgtgatct ggaaaactcc agcaacggtt
    15961 ccggtggcaa gatttctaaa gttgcagtca cctttgatag ctcagcgaaa accaccggcg
    16021 cagatccgat tctcaacaac accagcacgg gtgaagccac cggcgtcggc gtacgtttaa
    16081 tgaataaaga tcaaagcaat atcgttttag gcaccgctac tccagatatc gacctggctc
    16141 cgacctccag cgaacaaacg ctgaatttct ttgcctggat ggaacaaatt gatcaggcta
    16201 cacctgtaac gccaggcgca gttacggcga atgcaaccta cgtgctggat tataaataag
    16261 tattactttg gcggcgctat cgccgccttt cataaaatat ttatcaggta tggacaccgt
    16321 gaatatttat cgactctctt ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc
    16381 ggtcgaattc aaccttaatg ttctcgataa atcaatgcgc gaccgcattg atatttcatt
    16441 attaaaggaa aaaggagtca ttgctcccgg tgagtatttt gttagcgttg cggtaaataa
    16501 taaccaaatc agtaacgggc aaaagattaa ctggcacaaa aatgacgata aaaccattcc
    16561 gtgcatcaat gatttactgg tcgataaatt tggcttaaaa cctgaagtcc gtcagtcgtt
    16621 accattgata aatcagtgcg tcgattttag ctcccgacct gaaatgctct tcaatttcga
    16681 tcaagccaat cagcaactaa atatcaccat tccgcaagcc tggctggcgt ggcactcaga
    16741 aaactggacc ccaccctcca catggaaaga aggtgtcgcc ggtgtcctga tggattacaa
    16801 cttgtttgcc agcagctacc gcccacagga tggcagcagc agcactaatc ttaacgccta
    16861 tggaacagcc ggaatcaaca cgggggcatg gcgcttacgc agtgattatc agttgaatca
    16921 gactgatagc gatgataacc atgaacagtc aggcgaaata tcacgtacct atctttttcg
    16981 cccgttaccg caattaggtt caaaattaac cctcggcgaa acggatttta gttccaatat
    17041 tttcgacggt ttttcttata ccggcgcggc actggcaagt gacgatcgaa tgttgccatg
    17101 ggaactacgc ggctacgccc cacaaattag cggtattgca cagaccaatg ccacggtgac
    17161 gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc ccaccaggcc catttatcat
    17221 tgacgacctt aatcagtctg ttcagggcac actggatgtc aaagtgacgg aagaagatgg
    17281 tcgggtgaac aatttccagg tttcggcagc atcgacgccc ttcctgactc gtcagggaca
    17341 ggttcgctat aaactggccg cgggtcagcc acggccctcc atgtcacatc aaactgaaaa
    17401 tgaaaccttt tttagcaatg aagtttcctg ggggatgctg tcaaacacct cgctgtacgg
    17461 cggcctgctg ctttctggtg atgactacca ttctgccgca ataggtatcg ggcaaaatat
    17521 gctgtggctt ggcgcgctgt cgtttgatgt cacgtgggcc agtagtcact ttgatactca
    17581 gcaggacgag cggggcttaa gctaccgttt taattacagc aaacaagtgg atgccaccaa
    17641 cagcacgatt tccctcgccg cttatcgttt ctccgatcgt cattttcaca gctacgccaa
    17701 ctatctggat cacaaataca acgacagcga tgcgcaggac gaaaaacaga cgatcagctt
    17761 atctgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca
    17821 aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga
    17881 tattggtgac tggagagata tctctatttc gacgtcattc aatacaaccc attacgaaga
    17941 taaagatcgc gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg
    18001 ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga
    18061 tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtccgga
    18121 caatggagcc caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat
    18181 ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt
    18241 caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct
    18301 gatggtcagc accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa
    18361 ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt
    18421 gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg
    18481 gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt
    18541 catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga
    18601 cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc
    18661 tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc
    18721 tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg
    18781 aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac
    18841 gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt
    18901 ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat
    18961 gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa
    19021 ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc
    19081 gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac
    19141 agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg
    19201 gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag
    19261 ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga
    19321 cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg
    19381 gtcaacacaa acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat
    19441 tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg
    19501 gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc
    19561 cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa
    19621 aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc gataagattt
    19681 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaattttg
    19741 aaagtgagca tgtttatgcc tgggtaaacc cctacccggg ggtgcaggat cgctattatc
    19801 aactcggcgt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
    19861 acactaacca gtgtattgac agtaagaaca ttgacattta tacccctgag cagattatcg
    19921 cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgta
    19981 cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc
    20041 aaagtacgct tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag
    20101 accctactgc ccaaaacctg aaatatcata ttactggtct ggaaaacatt cgcgtgctgg
    20161 attgcagcgt caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta
    20221 atttgctgga tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat
    20281 cacaaaatga ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa
    20341 cgttggttga agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg
    20401 atgaaaacgc ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg
    20461 atatgctgat ctacgaaaaa acctatacgg ctgaactttc gtccattgca ggcaccccca
    20521 tcgaggctgg ccccttcgat acagtggtgc tttttaagat taactataac tgatttgcca
    20581 tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt
    20641 ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa
    20701 gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta
    20761 tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg
    20821 ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc
    20881 gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg
    20941 attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg
    21001 ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc
    21061 ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac
    21121 ggcgcagata ttcgcctggt ggcgtttatg caatatctgc gagtgctgtt cgtcgcgggg
    21181 gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa
    21241 atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt
    21301 gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg
    21361 gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg
    21421 gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta
    21481 ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt
    21541 tgtgcgggta tggcgtgggg gctgacccgg tttatgcaca ttgattttat gactgcctac
    21601 ctcgccacca gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc
    21661 gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacgggg
    21721 ccagccattg cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag
    21781 cctggacacc agtaaaacgg gcgcgaggac aacgtggttt tctcaatgat gccgccacag
    21841 cgttcgcaca gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc
    21901 ttattctcat ccacctgccc ccgcgtagcg taagaaaacc gaggaatctc cagtaacgcg
    21961 tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac
    22021 ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga
    22081 tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa ccgttccttt
    22141 accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga
    22201 tgcgtggtca gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg
    22261 gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg
    22321 gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taagtcgttg
    22381 gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata
    22441 agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg
    22501 cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg
    22561 gtgatatcct tttttcattc tttattaatc ggggttatgc tgcgacaaca atctcctttt
    22621 cggcaaatgt agagcgtagt cggcgggcga acgccagtgc gtgctcgccg tcgccatgca
    22681 ggcagactgt ttgcgccgtg accgttgccc attcgccggt gatacttttg actctgccgt
    22741 gttgcaccat ctctagcgtt tgcgccagcg cctgttcttc gttttcaatc aacgcgcctg
    22801 gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct
    22861 cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa
    22921 cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg
    22981 cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc
    23041 cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg
    23101 tttctggcgg caactgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt
    23161 gagcgccaat tgcaacacca ttttttattg cttcacgcac gcaagcctgc atggtttgcg
    23221 catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg
    23281 cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc
    23341 cacgctaatt gttcaaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt
    23401 gaacactgga caaaatgaat cggctgaccg agcggaattt gcgcaagatg gtacatatca
    23461 gcctcaatga tgcaggcaat acgcgggtaa ccaccggtgg tctgtgcgtc gttcatcaac
    23521 acaatcggct gcccgttatg tggcacctgc accacgcccg gtaacaaacc gtgagataac
    23581 agttcgcgat cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta
    23641 ctttgcgagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc acgatcgaac
    23701 tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg
    23761 ccctgcgctt ccataaaatc acgcttcgct ttgccaatcg ggagtcggtc accatccttc
    23821 agtaaacggc cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt
    23881 accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg
    23941 cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca
    24001 ttatcatcca gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg
    24061 aactcaacag ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc
    24121 accagtaggt tagcaatgcg taacgcgggc atatccagtg cgccgcagtg gctgataccc
    24181 gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatgcc cgcacgaata
    24241 atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata
    24301 agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc
    24361 agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac
    24421 ctgccggaac gagtaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac
    24481 tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg
    24541 agtgcaattc aacaacctgt ttttcgctca acccgcaatg cgccgcgacc accgccaaat
    24601 ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca
    24661 gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact
    24721 caggattacg caaaatcacc gtaatattgt tcatgccggg aatggcttca accacattcg
    24781 gcatatccac cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg
    24841 gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca
    24901 ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca
    24961 agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca
    25021 tggtgacctg cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttca
    25081 gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt
    25141 tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac
    25201 cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccag tcccggcaca
    25261 ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc
    25321 agtaacgccg ccagctgtgc gttattgccc agctcaggat gtgcgtcaag cggcaaatgc
    25381 cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg
    25441 cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg
    25501 cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt
    25561 tgcaccgtct ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg
    25621 ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc
    25681 gtattctgat agctacaccg ttaacatagc gttacctcgc cagttcgtcg atagccaatc
    25741 ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg
    25801 cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta
    25861 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgccg
    25921 tcaattgtgg aattattatg aataaacacg catcacagcc gcgcgctatt tactatgtcg
    25981 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct
    26041 atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact
    26101 gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
    26161 atcgcatggc ggtgatgctg ggggcgttat tgatggcgat cggtcatgtg gtgttgggtg
    26221 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gataatcgtt tgtggctatg
    26281 gtctgtttaa atccaatgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc
    26341 gtcgtgatgg cggtttctcg ctgatgtatg cggcgggtaa cgtggggtct attatcgcac
    26401 ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg
    26461 cggttggcat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcactcata
    26521 cccgcggcgt taataaaaaa gtactgcgtg cgacaaactt tctcctgcct aactggggat
    26581 ggctgctggt tctgctggtg gcaacgcctg cgctgattac cgtattgttc tggaaagagt
    26641 ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt
    26701 atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtg acgctcacct
    26761 tcttcagtat gttgttctgg gccttcgcac aacagggcgg cagctcgatt agcctttata
    26821 tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgactgct atgttccagt
    26881 cgattaatgc cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gttaaagaaa
    26941 gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt tgctcttggt ctcggcctga
    27001 tgagcgccgg gttctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact
    27061 cttctctgcc actgatggta ttaggcctgg cggtgatggg ctttgcggaa ctgtttatcg
    27121 acccggttgc catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa
    27181 ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg
    27241 atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat
    27301 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga
    27361 tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat
    27421 aggccagcac catacctgat aaaacgctcg ctgcgtttta tcaggctcct ttcctcgctt
    27481 cttcgtacgc tgccagcgtt cgcagccttg cctctttgtg atcaactatc ggttgcggat
    27541 aatccagctt cacacctgct ttctgcgccc acttccacgg ctcatgcacc gctttccctg
    27601 gtacatcgcg cagctccggt aaccatcgac gaataaactc gccctcacgg tcaaatttct
    27661 cgccctgagt tatcggattg aaaatacgaa aatacggcgc agcatcagta cccgtagaag
    27721 ccgcccactg ccagccaccg ttattagctg ccaaatcacc atcaatcagc tgcgacatga
    27781 aatatcgctc gccttcgcgc cagtcgatca acaaatcttt caccagaaaa ctggctgtaa
    27841 tcatccgtag ccggttatgc atccagccag tgctgttaag ctgacgcata gcggcatcaa
    27901 caatcgggta tcccgttttg cctttctgcc aggcctgtaa atgtgcggga ttgctttgcc
    27961 actgcacacg atccgtccag gcaataaatg gacgatgttt acacaacgag gggtaatacg
    28021 tcatcagatg acggtagaac tcgcgccaga tcagctcact aagccagaca ctaccggccc
    28081 caccgtccag cgcctgcggc tgttcagcca acaagcgatg caagcactgg cgaggcgata
    28141 acccgcccgt tgccaggctg gccgacaaac ggctggtgcc ttccactgcc ggaaaatctc
    28201 gttgttgctc ataatctccg gcaccgttct ggcaaaactg gcgtaattgc gcaatcgccg
    28261 ctttttcttc caccggaaaa tgcgcagtat cgaaagactg acgaggataa ttcagcgtaa
    28321 tggatggcga gggctctatc gatccgctac tacgaacttt tggtgcagcg acgcactccg
    28381 gcatcccttc c
//
LOCUS       DAFATA010000053        27937 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFATA010000053 DAFATA010000000
VERSION     DAFATA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.054"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(79..1158)
                     /gene="fbaA"
                     /locus_tag="LGJ20_002750"
     CDS             complement(79..1158)
                     /gene="fbaA"
                     /locus_tag="LGJ20_002750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBK2386786.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(1373..2536)
                     /gene="pgk"
                     /locus_tag="LGJ20_002751"
     CDS             complement(1373..2536)
                     /gene="pgk"
                     /locus_tag="LGJ20_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK2386787.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(2586..3605)
                     /gene="epd"
                     /locus_tag="LGJ20_002752"
     CDS             complement(2586..3605)
                     /gene="epd"
                     /locus_tag="LGJ20_002752"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBK2386788.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            3977..4408
                     /locus_tag="LGJ20_002753"
     CDS             3977..4408
                     /locus_tag="LGJ20_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK2386789.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            4431..5009
                     /locus_tag="LGJ20_002754"
     CDS             4431..5009
                     /locus_tag="LGJ20_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK2386790.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            5010..5717
                     /locus_tag="LGJ20_002755"
     CDS             5010..5717
                     /locus_tag="LGJ20_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK2386791.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPHSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            5705..6382
                     /locus_tag="LGJ20_002756"
     CDS             5705..6382
                     /locus_tag="LGJ20_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK2386792.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            6376..7053
                     /locus_tag="LGJ20_002757"
     CDS             6376..7053
                     /locus_tag="LGJ20_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBK2386793.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(7025..7738)
                     /locus_tag="LGJ20_002758"
                     /pseudo
     CDS             complement(7025..7738)
                     /locus_tag="LGJ20_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
     gene            complement(7735..8244)
                     /gene="fumE"
                     /locus_tag="LGJ20_002759"
     CDS             complement(7735..8244)
                     /gene="fumE"
                     /locus_tag="LGJ20_002759"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBK2386794.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(8266..9231)
                     /gene="yggF"
                     /locus_tag="LGJ20_002760"
     CDS             complement(8266..9231)
                     /gene="yggF"
                     /locus_tag="LGJ20_002760"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBK2386795.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(9228..10505)
                     /locus_tag="LGJ20_002761"
     CDS             complement(9228..10505)
                     /locus_tag="LGJ20_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBK2386796.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(10520..11908)
                     /gene="cmtA"
                     /locus_tag="LGJ20_002762"
     CDS             complement(10520..11908)
                     /gene="cmtA"
                     /locus_tag="LGJ20_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBK2386797.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(11936..12379)
                     /gene="cmtB"
                     /locus_tag="LGJ20_002763"
     CDS             complement(11936..12379)
                     /gene="cmtB"
                     /locus_tag="LGJ20_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBK2386798.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(12693..14684)
                     /gene="tkt"
                     /locus_tag="LGJ20_002764"
     CDS             complement(12693..14684)
                     /gene="tkt"
                     /locus_tag="LGJ20_002764"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK2386799.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            14962..15720
                     /gene="loiP"
                     /locus_tag="LGJ20_002765"
                     /gene_synonym="yggG"
     CDS             14962..15720
                     /gene="loiP"
                     /locus_tag="LGJ20_002765"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBK2386800.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(15926..16846)
                     /gene="speB"
                     /locus_tag="LGJ20_002766"
     CDS             complement(15926..16846)
                     /gene="speB"
                     /locus_tag="LGJ20_002766"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBK2386801.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(16982..17713)
                     /locus_tag="LGJ20_002767"
     CDS             complement(16982..17713)
                     /locus_tag="LGJ20_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386802.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(17859..19835)
                     /gene="speA"
                     /locus_tag="LGJ20_002768"
     CDS             complement(17859..19835)
                     /gene="speA"
                     /locus_tag="LGJ20_002768"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBK2386803.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(19844..19975)
                     /gene="yqgB"
                     /locus_tag="LGJ20_002769"
     CDS             complement(19844..19975)
                     /gene="yqgB"
                     /locus_tag="LGJ20_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBK2386804.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            20111..20326
                     /locus_tag="LGJ20_002770"
     CDS             20111..20326
                     /locus_tag="LGJ20_002770"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386805.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            20630..21784
                     /gene="metK"
                     /locus_tag="LGJ20_002771"
     CDS             20630..21784
                     /gene="metK"
                     /locus_tag="LGJ20_002771"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK2386806.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            22220..23614
                     /gene="galP"
                     /locus_tag="LGJ20_002772"
     CDS             22220..23614
                     /gene="galP"
                     /locus_tag="LGJ20_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBK2386807.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            23691..24188
                     /locus_tag="LGJ20_002773"
     CDS             23691..24188
                     /locus_tag="LGJ20_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBK2386808.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            24283..24990
                     /gene="endA"
                     /locus_tag="LGJ20_002774"
     CDS             24283..24990
                     /gene="endA"
                     /locus_tag="LGJ20_002774"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBK2386809.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            25070..25801
                     /gene="rsmE"
                     /locus_tag="LGJ20_002775"
     CDS             25070..25801
                     /gene="rsmE"
                     /locus_tag="LGJ20_002775"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK2386810.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            25814..26764
                     /gene="gshB"
                     /locus_tag="LGJ20_002776"
     CDS             25814..26764
                     /gene="gshB"
                     /locus_tag="LGJ20_002776"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBK2386811.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            26873..27436
                     /locus_tag="LGJ20_002777"
     CDS             26873..27436
                     /locus_tag="LGJ20_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBK2386812.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            27436..27852
                     /gene="ruvX"
                     /locus_tag="LGJ20_002778"
     CDS             27436..27852
                     /gene="ruvX"
                     /locus_tag="LGJ20_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK2386813.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
BASE COUNT         6726 a         7475 c         7028 g         6708 t
ORIGIN      
        1 gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg ggccttgaga
       61 taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga atgctttttc
      121 cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat cgtagtattt
      181 cttgttcggc tgatcttcgc ctttcgggtt acccagctga ccctgcagat aagcttcgtt
      241 cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg tatcgatgtt
      301 cattttcact acgccgtagc ttacggagtc tttgatttcc tgagcagtag aaccggaacc
      361 accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttag aaacatattc
      421 ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt aaacaccgtg
      481 tacgttaccg aaggacgctg cgatggtgaa acgcgggctg attttgctca gctcggtgta
      541 tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt ggctgttgtc
      601 cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca tgccgatttt
      661 ggacatacgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag attcttcaga
      721 caggtcgatc atgtgagaag agaacagcgg cttgccggta gcagcgaagt gtttttcacc
      781 cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt cggtgtgcag
      841 gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac cagagatcgc
      901 gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag cgataaagga
      961 agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag cggtttccag
     1021 tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga agttgttttc
     1081 ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag gttttacgaa
     1141 atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact cgtcatactt
     1201 caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat gtaagctcct
     1261 ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat gaaaaaatgc
     1321 gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt ttttacttct
     1381 tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg aattcgagga
     1441 atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac aggtcgattg
     1501 ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg atagcgttag
     1561 ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc ggaccgttcc
     1621 acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa gcatcaccga
     1681 tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta gccggtgcag
     1741 tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg caggtggtca
     1801 gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc acatcgtggc
     1861 cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg tcagcgattt
     1921 tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca acgatagcca
     1981 ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca gctgccagca
     2041 gcgggcctgc gcacgcaacg tcagcgaatt taccgatacc gtgagtagaa gcctgcgcgc
     2101 ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg tattttttgg
     2161 acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc agaacaacca
     2221 gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga accgggttag
     2281 acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg ttgtactcgc
     2341 cttcggtagg acgacccagg tgggaagtta ccatcacttt tgcgccttgt ttcagggcca
     2401 gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc ccgtctttta
     2461 ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc agatcggtca
     2521 tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca gacgctgctt
     2581 gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat tcggttagca
     2641 aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc actgacccgg
     2701 gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac agagaccaac
     2761 ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt ttgcagcaac
     2821 aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc gattgccgtc
     2881 acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa ttgcggaaaa
     2941 aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg gctggctgcc
     3001 cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg catggcggag
     3061 tgaattgtgg tcactgtgcc ggactcaata ccgtacgcat catctaacaa tttgatgacg
     3121 ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc cgcacgaagt
     3181 tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc aggatgtgaa
     3241 aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg ggagccatat
     3301 acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag cgattgcagt
     3361 gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc gcgttcctgt
     3421 cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg cgccatgccc
     3481 gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg tccggattca
     3541 tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc tacgcgtacg
     3601 gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt aatccagcaa
     3661 atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt gtcgaacatt
     3721 taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg aatgattgtc
     3781 cagtcaaaga aagcaattat ctgacttacg tcacgttttc agccccgccc ctgtggaatt
     3841 tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt gccgtcacaa
     3901 accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca atacgtccga
     3961 gtttcggagt actccaatga tgacattgcc ctacccatat tcttcctccg cacgctttta
     4021 cgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt ttgcacagca
     4081 gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg acgtcgcctt
     4141 gcgctatgcc gggcaagaag gcaccacgct cctgaacggt acattcgaag tgatctcact
     4201 aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg atccacacgg
     4261 cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc ttgaactggt
     4321 gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt ctggttacga
     4381 cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt atggcgcgta
     4441 gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc aatatgatcg
     4501 gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc ggcacactta
     4561 ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg accaatttac
     4621 tctggggatt gctgaccaac ccgatcgccg ccgcatttgc acccgtcgcg atggtcatcg
     4681 gcctggtcgc cggttgcctg gcgcgagcgg gttggtttcg caccctgcca aaagtcgttg
     4741 tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg cgtactgcgc
     4801 tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg cactcgatgg
     4861 ggcaaaacct ggtggagtcg gtggcgatta cggtgatagg cgcgaacctg gtcgataaaa
     4921 ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc accacgcgcc
     4981 attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta acactgtggg
     5041 cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgcca atctatagcg
     5101 cggcgacctt tttctgcctt attgcgctaa aggccacgcg gcggcgggca aaatatgtcg
     5161 tctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc tggctaccag
     5221 aatggctaag cggcacgcca cactcgccag aacggtggtc gcatgctatt acgctctggt
     5281 tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc cccgtgcaat
     5341 gttttattcg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat ctgtttgccg
     5401 ggccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat gaagcgcagc
     5461 gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg atgcccgcgt
     5521 taattattcc gctaacacac aatgcgctta acgatctggc tgtccgtggt gcggcgctgg
     5581 atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca gccgatagca
     5641 ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctgacagaa ttcggagcgt
     5701 ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc atcctccgtt
     5761 ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg acaacggcag
     5821 tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc tgccaggaga
     5881 gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc ttaatgaaca
     5941 atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg gctgtacctt
     6001 tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg aagcagagat
     6061 tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc gccatcgtca
     6121 cccggcaaca ctctccggtg gagagacaca gcgcgtggtc atcgcctgcg ccatcgcgat
     6181 gcagccaaag ctcttaattc ttgatgaagc ctttagccgc ctgactccgc aggccagcga
     6241 aatgctgttg cagcgcttac aacactgggc gtttgaacgt ggttcgctga ttattctttt
     6301 tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat tgcaaaacgg
     6361 agcgctccag ccactatgtt aacgttaaat aagatatctt atcgctggcc tggcgcggca
     6421 acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt ggcgctgacc
     6481 ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct tcttacgccc
     6541 acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa ccgccagcgc
     6601 gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca cagcaccgtc
     6661 gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga aatcactcaa
     6721 cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc gcacccgttg
     6781 gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc actctctccg
     6841 ccactattgc tgctggacga gccgagtcgg gattttgacg aaaattggtt gagcgtgttt
     6901 gaaagctggc tggagaagtg cggccagcga ggcacgagcg tcgtagcaat cagtcatgac
     6961 gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg tctaatcagg
     7021 aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc aaatttgcag
     7081 gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca taaaaagctt
     7141 ctgcgtctgc cagcgatacc cctcccgcca gcttgcgccc taccagtcgt tcccgcaacg
     7201 cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc tggcaccact
     7261 tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg gtaacgtgca
     7321 acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta caatctcctt
     7381 ccacgaccta gcgcagattt tccgccagtt tcacaacgtc aaatgtctct ggtgcgcctt
     7441 tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa ccatccatcg
     7501 ggagcgtctg aatagcgggc agttccgggt cttgttgcgc gagatattcc cagaaggtag
     7561 ttaaggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta cgtcgctgcg
     7621 aacttaccgc ctgtaaggcc gccagcatac gcagtagcgg tttatgcacg cgttctatct
     7681 cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca attttcacgg
     7741 cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga ccattccgct
     7801 aaaacgcgcc tctgaccaga cggcgaaatc ggcgaacttc atcgctttta acgcatcata
     7861 gagcgtcgca ttgcaggtta tgctattgac gttgctgata aaatcccagg tcatgtcatc
     7921 ggcaaatccg ggcgtttcat cctgttcgtt caggtaatgc cagtactggg aaaagtgcgt
     7981 gatatcggca tacagccatt tatccatttt ccccagcgca taaatcaaac gcagcgcaac
     8041 atcaatatcg tcaagcgggc cgctttgggc gagtaacggc ttcactgcat attcgacgat
     8101 ctcttcatcg ttatcgacaa acagcgacgg cagaaactgg cgtatcccct ggagcaaaat
     8161 agaatgcgca gttttcataa atgaaaataa attgtcctgt gcatccagtt gctcaagcac
     8221 atcatcttct gtcagcgtcg ccattggtct tccgatagat caccatcaat gcagggagtc
     8281 tattatatta cacgtttggt ccgcgccgcc gatcagtaat gtctgcgtcc gcaccccatt
     8341 cgccgtctgt tggataccgt tcaccaactc gccgcccgtc acgcccgtag cgctaaagag
     8401 gatatcgttc cccctcacca gttcatcgag cgagtacaca cggttaacgt caacgcccat
     8461 tgccttacaa cgcttgcgct cctgctctgc aatctgccga ttttccgtgt aatccccttt
     8521 agcctgacaa aagtcaatca gctcggcctg catatcccca cctaatgctt taacggcaca
     8581 ggcagaaatc acgccttctg gcgcaccgcc gatggtgtac atcacgtcgt aaggattatc
     8641 ctgccagcag gtcagcacgc tggcggcgac atcgccatcc ggcagagcga aaaccttcac
     8701 gcccagctgt gttgcttctt caatcgcggc actcaggcgc ggtttatcca gcgtaaccat
     8761 gcgcagcttg tccagcggtt tacctaacgc ccgcgccacg ttacgcaagt tgtccgccag
     8821 aggcaaagac agatcaatcg ccccggcagc cagtcgatta acaaccagct ttttcatata
     8881 catatcgggc gcatgtaaca ggctatctcg aggggcgaaa gccatgaccg ccagcgcatt
     8941 gctctgcccc atcgctacca tccgcgtgcc ttcaatcggg tcaaccgcga tatcgacttc
     9001 tggcccatcg cctttgccca cctcttcgcc aatccacagc atcggcgcat ggtcaatttc
     9061 tccttcgccg ataaccactc gtccacgaaa agcgacgtcg tttaatgcct ggcgcatcgc
     9121 ggtgaccgcc aggccatcaa ttttattttt gtcgccacat ccggtttgcg gccaggcagc
     9181 caacgctgcc tgttccgtaa cgcgaaataa tggccacgcc agggacatca ttgcgcggcc
     9241 tccccgatat caacgccaaa ttgtgccagc agatatttct cggcctgttc attccagatg
     9301 ccgtgcgttt cctcaaccag ccgcgccaac tctttaaaca gcggatcggt tttgcctttt
     9361 tcggcaaaat cggcaatggc agtgagcggc atggtcacgc cgttataaat gagtttttta
     9421 ccgccaggga tatccggcag attgagcacg gtatctggca ccgcatccag cccaccgata
     9481 tgcgtgacca taaaggacgg ctgcaactgc cccgtggcgc ttaacgcaat cgcctctttc
     9541 atgtcgtccg ttgaaccgcc ggatgtaccg actacgtgcg tgctgttgta atggacgttg
     9601 tagaaattaa acggcacttt gaagttttta tccgtcggcc cggcaaagaa gttcagacag
     9661 ccatcttccg ccagtaattc gtcagccatc tcaacgacag caggcaccgc cgcataaaca
     9721 aaaatgtcat cgaacccgac atctcctgtc agcgcccgca gcgtctggac cggatcgctc
     9781 atccctttgg tattcacata caccagctca atgcctttac tggccgccag atccaccggc
     9841 agcagcttct gcacctgcgc cagacgtttg tcgtcgatat cgaccaccac cacccgcgac
     9901 ggttgtatgc cgccgttaat ggcgtaatcg atagcgccaa tgcccatcgg acctgcacac
     9961 gccagcagtg caatattgcc gccaggtttg acgcccatgc gatgctcata aacatattgc
    10021 gtggtgtgat aattggcatg ataagcacca ataatgcagc acataggctc tgccagcgac
    10081 gccgcagcaa aataagagcc gtgatacggt aatacgcagc ccaaattaat ggcgatttcg
    10141 ggaataatca tataagtggc attgccgcca aaatattcgt agctgtagcc cgctgaatat
    10201 ccgcttggta accccatcgc tggttgcaat acaaaacgct ggcctttttt atatttgcca
    10261 gtgagatttt tacccacttc gacaataacc ccggcacatt catgcccggt aatgaccgga
    10321 tgattttcta aatcgtcggg tacgcgttta tgttcactac cgagtaacgc agctttccag
    10381 gtggataaac agacgctgtc agaaattaca ctcaccagta attcattatc ggtaatttct
    10441 ggcagttcaa attcgcgcag acggacatcc cgcttgccat aaatagcagc aactttggtt
    10501 ttcattttta cctctgtaat cagtgtttat gttcggcggt taattgatta aataaagtgt
    10561 cgagttttgg atcgccaata tagttattaa tcaatatcag cggtttatcc gtaacacgtt
    10621 tcacgcgccc ttccagactg gcatgagtaa cgacgatatc ggcatccgca ggcacgtttt
    10681 ctatcgcgta atgttttact tctattgcca gccccgcttt ttccaggcgt ttacggaagg
    10741 tggtcgcgcc catcgcactg gagcccatcc ccgcatcgca aacaaaggcg atacgcttaa
    10801 ccctgcttaa cgagaatgca ccctcttgtt tcatcgcctt aaccgcattg gctgactgag
    10861 caaactcatc ttcgctctcc gtttccaccg ttttttccat cttcagtatc agcgaagtaa
    10921 tagcaaagga caccagggta cctaccgtaa caccggcaat tgtcgccagg aacgagcctt
    10981 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
    11041 cgtccagtaa gttaaacatc caggtaccag acataccgcc cgcaatcatg gcaataatag
    11101 tcagcggctt catcagcaca tatgggaaat acagttcgtg gatcccaccg aggaagtgaa
    11161 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
    11221 gcagcagccc caggcccgga cctgggttag aggccaccat aaagaagatg gatttaccgt
    11281 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattaa
    11341 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
    11401 ccagcgcctc gatacactct ttgacgaaag tattggcaat taacaccgcc gggccgatca
    11461 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
    11521 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcgacatat tttatgacca
    11581 acccaccgag cggccccatg atcattgagc caaggaacat cgggatctct gcgccaacga
    11641 tcacaccaat agtacctatt ccgcccatga ccgccccgcg tttaccgccg accagatgac
    11701 cacctgtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
    11761 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
    11821 caataaaagc accaatattg gggatgacca ttgcagtcaa aaagccccca aaagcctgga
    11881 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
    11941 cgcggctgat aatgtccgct aattgttttt ctgatgatgc tgttaagagt tgttcgagta
    12001 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
    12061 cggcagcaga gagtccgatg agtaatttaa ttggttcgtc attccccgga aaataaacac
    12121 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
    12181 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
    12241 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
    12301 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
    12361 aataatcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
    12421 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg atgttttgaa
    12481 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
    12541 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
    12601 tacaacacga tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
    12661 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaaca
    12721 acgttatcaa cagtgaagcc gaactcttca aacagcagct ctgccggagc agattcaccg
    12781 aaggtggtca taccgacgat agcaccgttc aggccaacat acttgtacca gtagtcagca
    12841 atacccgctt ctacagcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
    12901 gcagcatcct gcttgtcaaa tgcgtcggta gacggcatgg acaccacgcg cgctttcacg
    12961 ccttcggcag tcagtttttc gtaggcagca acagccagtt caacttctga accggtagcg
    13021 atgaaaatca gttccggctg accggcgcag tctttcagca cataaccacc gcgcgcgatg
    13081 tttgccagtt gctcttcagt tcgttcctgc tgcgccaggt tctgacggga gaggatcagt
    13141 gcggtcgggc cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
    13201 tcacacggac gccatgtaga catgttcggg gttacgcgca gagaagcgac ctgctctacc
    13261 ggctggtgag tcgggccatc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
    13321 tgacgctgtt tcatcagcgc agccatacgt acggcgttac gtgcgtattc cacgaacatc
    13381 aggaaggtgg aggtgtacgg caggaaacca ccgtgcaggg agataccgtt agcaatcgcg
    13441 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
    13501 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcaga accgccgagg
    13561 aattccggca acagcggacc gaaagcttcg atagcattct gagacgcttt acggctggcg
    13621 attttcgccg ggttagcctg cagtttagca atgaactctt ttgctttcgc gtcgaagtca
    13681 gacggcattt cgcctttcat acggcgggta aattcagcgg cttcctgcgg ataagctttc
    13741 gcgtaagcag cgaatttctc gttccatgcg gattctttcg cctggcctgc ttctttcgca
    13801 tcccactgag catagatttc agacgggatt tcgaacggtg catatttcca gcccagctgt
    13861 tcgcgggtca gggcaatttc agcgtcgccc agcggcgcac cgtgggagtc gtgagtaccg
    13921 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
    13981 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ccgcgtcatg accgtcgatg
    14041 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
    14101 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
    14161 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
    14221 tcgcccatga aggcataggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
    14281 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
    14341 ggaccggtgg tggtttccac accagcggtg taacccactt ccgggtgacc cggagtttta
    14401 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
    14461 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
    14521 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
    14581 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
    14641 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
    14701 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
    14761 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
    14821 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
    14881 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
    14941 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
    15001 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
    15061 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
    15121 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
    15181 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtaaa ttacaaagtg
    15241 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
    15301 gggctgatgg atatgatgac ggataacgaa gtcgaagcgg tgatcggtca cgaaatgggg
    15361 cacgtggcgt taggccatgt gaaaaaagga atgcaggtgg cacttggtac aaatgccgtg
    15421 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaactcggt
    15481 gatctgggcg agaaattagt taattcgcaa ttctcccagc gccaggaagc agaagccgat
    15541 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
    15601 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatcct
    15661 gcatccgccg aacgcgccca gcatattcgc gatcgtatga gcgcggatgg gataaagtaa
    15721 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac cccgcatctg acatgaatgg
    15781 cacatttgtt accttgtgcg cattgccgga tgcgatgctg gcgcatctta tccggcctac
    15841 gggtgccgaa cgtaggtcgg ataaggcgtt cacgccgcat ccgacattaa tggcacgttt
    15901 tacccgtgcg catcgcatct ggtgcttact cgcccttttt cgccgcctga atatacagca
    15961 tctccagcgc cagcgtcgcc gcagccagag cagtgatttc cgactgatcg tatgccggag
    16021 ccacttccac tacgtccatc ccaacgatgt tgagatcttt caggccgcgt accagtttaa
    16081 tggcgcgatc ggaggtcaga ccgccaatta ccggcgtacc ggttcccggc gcaaatgcag
    16141 gatccaggca gtcgatatca aaggtcaggt aaaccggcat atcacccaca atttgtttca
    16201 cctgggcgat aacgtcatcc acgctgcgat cgttcacctg gcaggcgtcc agcacggtaa
    16261 agccgttgtc tttatcaaat tcggtacgaa taccaatctg cacggaatga ttcggatcga
    16321 tcagaccttc tttcggcgcg gtatagaaca tggtgccgtg gtcaaattca caaccgttcg
    16381 cgtaggtatc ggtgtgggcg tcaaagtgta ccagcgccat tttgccgaaa tgcttcgcat
    16441 gagcacgcag cagcggcagc gtaacaaagt ggtcaccacc gaaggagagc atacgcttac
    16501 cggcagccag cagcttctcg gcgtgcgcct gcagcttctc gctcatctca cgggcatcgc
    16561 cgaaagcata taccagatca ccgcagtcca cgacgttcag acgctcacgc atgtcgaaat
    16621 tccacgggaa gcggttgtgt tcccaggcca gattcgtcga aacctgacgg atagctgccg
    16681 gaccgtggcg tccccccgca cgaccagaag tggccatatc gaacggcaca ccagtaatca
    16741 cccagtcagc atcgctgtca tacggctgga agttcatcgg caggcgtaaa aaaccaaagg
    16801 cattggaaac cagtgagtta tcgtattgat gacctaaggt gctcatggat ggactcctat
    16861 tacaaagtcg atataaaaaa acccttccgc gtcgttaggc ccgacgagga agggttggat
    16921 ttgcgaaaca caagtttgtg ctaattatcg ccttaaatgg gcgaggcatc aagcgaggtt
    16981 gctatttccg acacggtggc ggcgcatagc cattgttatc tgccggaaag gcttgtccag
    17041 agcacggcgg cagcgcgttt tgcgcctggc cttgtaattc aaaggcggca aagcctatta
    17101 caccggtatt acgcacatcg ccttgcgccg aattggctgc gtaggaatca gcaacgttgg
    17161 cgaactgaaa agccgcttcg gtatgtttat ctttgcggaa tcctttaatc gctaaagaat
    17221 ctccggcatt cacaatatag ccgttgttat tcagcgaacc ttgcttgccg ttcagcacat
    17281 ccaggccatc aacagtagca acgatctcgt agttggtgtt acgactgtaa ttacggacat
    17341 ataattgata attcattccg actctggcat ggacaatata gcgtggttca ttattggatg
    17401 aatcataaac acgcgtaata ggtagagaat taaaattacc atcgcgaata gcatattcca
    17461 gttcgttaat acgaatgcta taaacgcggt catagccgga aggaacatta gtcgaataac
    17521 taataacgat catctctgcc ggatcgcgcc aggctcgctc agcagatacg ccctgaaccg
    17581 atgagtgcac atccccaccc caggcagtgc cgacattgcc cgatgcactg ctattatact
    17641 gcgcattact ggaacaaccg gccagcagca cgattaatgc caccaacgcg ctacgtactt
    17701 tccatttttt cataaccgtt ccctctgcat ttaattaatg cagcgattgt agggagatta
    17761 tttaataaga aaagcagcga gattattttt aagaatatat aaaaagaaag cgggccgatt
    17821 tacgtaaata aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataggta
    17881 taaccgtaca aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga
    17941 tcggttttct tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca
    18001 tattgcagca tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg
    18061 tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga acaggttgtg catattgcca
    18121 aggatctcct gatatgcgcc gaccataaag aaaccgagca tcggcggatt ctctggatcg
    18181 tactccggca ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca
    18241 ccgtcagagt cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc
    18301 ccttccagcg gcagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg
    18361 aacagcgaga aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc
    18421 ggacgatgcg cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga
    18481 tagagttgtt ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata
    18541 tgaatgtcgt gcagatccat ctgactgtcg tgtaaccatt cacgcagaga acggcgagtt
    18601 cccggttcgt gcatctcctg ccaggtttcc cacatgcttt gcagcgcgcg cggcgcatct
    18661 tctgcaggcg cggtcggcac cgtgtattcg ttacgttcca cgccgatgat attagacacc
    18721 agcacggtgt gatgcgcagt caccgcacga cccgattcgg tgattaccgt cggatgcggc
    18781 agaccgtttt cttcacacgc atcaccaatc gcccagatga tgttgttggc atattcattg
    18841 aggccgtagt ttacggagca gtcagactgc gaacgggtgc cttcatagtc cacgcccaga
    18901 ccgccgccga cgtcgaagca ctgaatattg acgcccagct tgtgcagttc cacatagaaa
    18961 cgcgccgatt cacgaacgcc tgtggcgata tcgcgaatat tcgccatctg cgaaccgagg
    19021 tggaagtgca gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt
    19081 tgcagtacct gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat
    19141 ttacccgagc cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga
    19201 cgttctgctt catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc
    19261 ttgtgcccca tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg
    19321 ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca gcactgccat caactcggct
    19381 ttggaaccgg cttccagacc cagcggttcg cccgaatgaa tcagggactc aatcacgcga
    19441 cggtgctggt taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat
    19501 tcccgcgcac gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg
    19561 aaacagaaca gtgcaggcag acgctggccc tgtgcttcac gagttttcac taactgcgcg
    19621 agatcgacgc gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg
    19681 ttaacgtcat agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg
    19741 gcttcctggg agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct
    19801 gacgaaggca aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa
    19861 gtgtaaaaca gttaacgact atcgcagcct ggaactgcga taacaaccca taagcacatg
    19921 gattttccag cagtgaatgc tgacgctcca actgcgcaac cggtttcttt ttcataacat
    19981 tattaagcac ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg
    20041 tttttacaag agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag
    20101 aagcgccgag atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga
    20161 acgaatcttc accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt
    20221 atcacctcca cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg
    20281 gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt
    20341 tttatacccc acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc
    20401 aggaaaaatt tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa
    20461 tcgggtgaag aattgaccta aaatagccat ccagatgtta atccatccat accgattaac
    20521 actcagactg ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa
    20581 tccacacaac agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca
    20641 cctttttacg tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc
    20701 tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac
    20761 ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga
    20821 catcgaagag atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg
    20881 ctttgacgct aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa
    20941 ccagggcgtt gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt
    21001 tggctacgca actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg
    21061 tctggtacag cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga
    21121 cgcgaaaagc caggtgactt tccagtatga cgacggcaaa atcgttggta tcgatgctgt
    21181 cgtgctttcc actcagcatt ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat
    21241 ggaagagatc atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt
    21301 catcaacccg accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg
    21361 tcgtaaaatt atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc
    21421 tggtaaagat ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa
    21481 aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg
    21541 cgtggctgaa ccgacttcca tcatggtaga aactttcggt actgagaaag tgccttctga
    21601 acaactgact ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat
    21661 gctggatctg ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga
    21721 acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa
    21781 gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc tggccttttt cttttacctc
    21841 accgcggtgg ataggcattc acgccgcatc cggcaaaaag ctgcccgcac aataacatca
    21901 ttcttcctca acacgtttta tcgcaaatta tcatcactac tgaaaccgat tacaccaata
    21961 acaccagcca aatatttgta atattttcat attattattc agttttcaca gttgttacat
    22021 ttcttttcag taaagtctta attgcagata acagcgttta atctatgatg atataactca
    22081 attattttca tgcacttaaa ccataactaa gataaatgtt agtgtaagcg attacactga
    22141 tgtgatttgc ttcacatctt tttacgtcgt actcacctat cttaattcac aataaaaaat
    22201 aaccatattg gagggcatca tgcctgacgc taaaaaacag gggcggtcaa acaaggcaat
    22261 gacgtttttc gtctgcttcc ttgccgctct ggcgggatta ctctttggcc tggatatcgg
    22321 tgtaattgct ggcgcactgc cgtttattgc agatgaattc cagattactt cgcatacgca
    22381 agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc ggtgcggtgg gcagcggctg
    22441 gctctccttt aaactcgggc gcaaaaagag cctgatgatc ggcgcaattt tgtttgttgc
    22501 cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta ctgattcttt cccgcgttct
    22561 actggggctg gcggtgggtg tggcctctta taccgcaccg ctgtacctct ctgaaattgc
    22621 gccggaaaaa attcgtggca gtatgatctc gatgtatcag ttgatgatca ctatcgggat
    22681 cctcggtgct tatctttctg ataccgcctt cagctacacc ggtgcatggc gctggatgct
    22741 gggtgtgatt atcatcccgg caattttgct gctgattggt gtcttcttcc tgccagacag
    22801 cccacgttgg tttgccgcca aacgccgttt tgttgatgcc gaacgcgtgc tgctacgcct
    22861 gcgtgacacc agcgcggaag cgaaacgcga actggatgaa atccgtgaaa gtttgcaggt
    22921 taaacagagt ggctgggcgc tgtttaaaga gaacagcaac ttccgccgcg cggtgttcct
    22981 tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg aacgtcatca tgtattacgc
    23041 gccgaaaatc ttcgaactgg cgggttatac caacactacc gagcaaatgt gggggaccgt
    23101 gattgtcggc ctgaccaacg tacttgccac ctttatcgca atcggccttg ttgaccgctg
    23161 gggacgtaaa ccaacgctaa cgctgggctt cctggtgatg gctgctggca tgggcgtact
    23221 cggtacaatg atgcatatcg gtattcactc tccgtcagcg cagtatttcg ccatcgccat
    23281 gctgctgatg tttattgtcg gttttgccat gagtgccggt ccgctgattt gggtactgtg
    23341 ctccgaaatt cagccgctga aaggccgcga ttttggcatc acctgctcca ccgccaccaa
    23401 ctggattgcc aacatgatcg ttggcgcaac gttcctgacc atgctcaaca cgctgggtaa
    23461 cgccaacacc ttctgggtgt atgcggctct gaacgtactg tttatcctgc tgacattgtg
    23521 gctggtaccg gaaaccaaac acgtttcgct ggaacatatt gaacgtaatc tgatgaaagg
    23581 tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct caggcttcct cccatcgcgg
    23641 aggaagccac ctcttgcagt catcttttct tcgctctatc ctctgccgct atgaaaacat
    23701 cccgtctccc tatcgccatc cagcaggccg ttatgcgccg cctgcgggaa aaactcgccc
    23761 aggccaacct gaagctaggg cgtaactacc cggagccaaa actctcttac acccagcgcg
    23821 gaacctccgc cggaacagcc tggctggaaa gctatgaaat ccgcctcaat cccgttttgc
    23881 tgttggaaaa cagtgaagct tttattgaag aagtggtacc gcacgaactg gcacatttgc
    23941 tggtatggaa acatttcggc cgcgtagcgc cacatggcaa agagtggaag tggatgatgg
    24001 aaagcgtgct gggcgttccc gcccgtcgta cgcatcagtt cgaactgcaa tccgtgcgtc
    24061 gcaacacctt cccctaccgc tgcaagtgcc aggagcatca gcttaccgta cgccgccata
    24121 atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca ctgcggtgaa cagctggttg
    24181 cgaaataacc atctgaacta tcaggaactt tcctgatctg gctgattgca taccaaaaca
    24241 actttcgcta cgttgctggc tcgttttaac acggagtaag tgatgtaccg ttatttgtct
    24301 attgctgcgg tggtactgag cgcagcattt tccggcccgg cgttggccga aggtatcaat
    24361 agtttttctc aggcgaaagc cgcggcggta aaagtccacg ctgacgcgcc cggtacgttt
    24421 tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg ttgttgatct gcaatcgtgc
    24481 ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg tagagtggga acacgtcgtt
    24541 cccgcctggc agttcggtca ccagcgccag tgctggcagg acggtggacg taaaaactgc
    24601 gctaaagatc cggtctatcg caagatggaa agcgatatgc ataacctgca gccgtcagtc
    24661 ggtgaggtga atggcgatcg cggcaacttt atgtacagcc agtggaatgg cggcgaaggc
    24721 cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa aagctgccga accaccagcg
    24781 cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc gcgaccaata caacctgaca
    24841 ctctctcgcc agcaaacgca gctgttcaac gcatggaaca agatgtatcc ggttaccgac
    24901 tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag gcaatcataa cccgtatgtg
    24961 caacgcgctt gccaggcgcg aaagagctaa cctacactag cgggactctt tttgttaacc
    25021 cctaccccac gcgtacaacc gcgtggggag acgacgcgga tttttaacta tgcgtatccc
    25081 ccgcatttat catcctgaac cactgaccag ccattctcac atcgcgcttt gcgaagatgc
    25141 cgccaaccat atcgggcgcg tactgcgcat ggggccgggg caggcgttgc aattgtttga
    25201 cggtagcaac caggtctttg acgccgaaat taccagcgcc agcaaaaaaa gcgtggaagt
    25261 gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg ctgcatattc acctcggtca
    25321 ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag aaatcgatcg aactcggtgt
    25381 aagcctcatt acgccacttt tttctgagcg ctgcggcgtt aaactggata gtgaacgtct
    25441 gaacaagaag ctccagcagt ggcagaagat tgcaattgct gcctgtgagc agtgtggtcg
    25501 taaccgggtg ccggaaatcc gcccagcgat ggatctggaa gcctggtgtg cagagcagga
    25561 tgaaggactg aaactgaatc ttcacccgcg cgccagtaac agcatcaata cgttgccgtt
    25621 accggttgaa cgcgtccgcc tgctgattgg cccggaaggc ggtttatcgg cagatgaaat
    25681 tgccatgact gcccgctatc aatttactga tatcctgttg ggacctcgcg ttttgcgtac
    25741 agagacaact gcgctcaccg ccattaccgc gctacaagta cgatttggcg atttgggcta
    25801 acggagaaga ataatgatca agctcggcat cgtgatggac cccatcgcaa acatcaacat
    25861 caagaaagat tccagtttcg ctatgttgct ggaagcacag cgtcgtggtt acgaacttca
    25921 ctatatggag atgggcgatc tgtatctgat caatggcgaa gcccgcgccc atacccgcac
    25981 gctgaacgtg aagcagaact acgaagagtg gttttcgttc gtcggtgaac aggatctgcc
    26041 gctggccgat ctcgatgtga ttctgatgcg taaagacccg ccgtttgata ccgagtttat
    26101 ctacgcgacc tatattctgg aacgtgccga agagaaagga acgctgatcg ttaacaagcc
    26161 gcagagcctg cgcgactgta acgagaaact gtttaccgcc tggttctctg acttaacgcc
    26221 agaaacgctg gttacgcgca ataaagcgca gctaaaagcg ttctgggaga aacacagcga
    26281 catcattctt aagccgctgg acggtatggg cggcgcgtcg attttccgcg tgaaagaagg
    26341 cgatccaaac ctcggcgtga ttgccgaaac cctgactgag catggcactc gctactgcat
    26401 ggcgcaaaat tacctgccag ccattaaaga tggcgacaaa cgcgtgctgg tggtggatgg
    26461 cgagccggta ccgtactgcc tggcgcgtat tccgcagggg ggcgaaaccc gtggcaatct
    26521 ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa agtgactgga aaatcgcccg
    26581 tcagatcgga ccgacgttga aagaaaaagg gctgattttt gttggtctgg atatcatcgg
    26641 cgaccgtctg actgaaatta acgtcaccag cccaacctgt attcgtgaga ttgaagcaga
    26701 gtttccggtg tcgatcaccg gaatgttaat ggatgccatc gaagcacgtt tacagcagca
    26761 gtaacccacc ttagcgagaa ggatctcgtt gagactctga gtgacagcgc ccttctttcc
    26821 acgcatactg ggcgctgttg cttttttgaa ccaggaaaca gaacctctga caatgaattt
    26881 acagcatcac tttcttattg ccatgcctgc tctccaggat ccgattttcc gtcgttccgt
    26941 ggtctacatt tgcgaacata ataccaatgg tgcaatgggg atcatcgtca acaagccgct
    27001 ggaaaatctc aaaattgaag ggattctgga aaagctgaag atcacgccgg agccgcgtga
    27061 tgaatcaatc cgtctggata aaccggttat gctcggcggt ccgctggctg aagatcgcgg
    27121 gtttattttg catactccgc cctccaattt tgcttccagc attcgcattt cagacaacac
    27181 agtgatgacc acttcccgcg atgtgctgga aacgctcggc accgataaac aaccgtctga
    27241 cgtattggtg gctctgggtt atgcctcctg ggaaaaaggt caactggaac aagaaattct
    27301 cgataacgcg tggctaacgg ccccggcgga tctgaatatt ctgttcaaaa cgccgattgc
    27361 cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat attctcacca tgcctggtgt
    27421 ggcagggcac gcctgatgag tggaacctta ctcgccttcg acttcggcac caaaagcatt
    27481 ggcgtagcgg tcggccaacg cattaccggc accgctcgcc ctttgcctgc aattaaagca
    27541 caggacggta cgccggactg gaacattatc gagcgtttac tgaaagagtg gcagccggac
    27601 gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg agcaaccatt gactgccaga
    27661 gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg ttgaagtaaa gctccatgac
    27721 gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg aacagggcgg ctatcgggcg
    27781 ctcaacaaag gcaaagtcga ctctgcctct gcggttatta ttctcgaaag ctatttcgag
    27841 cagggatatt aaggcgatct aaaagcctgg cggatagtcg ttaccgccat cttgccggat
    27901 gcggcgtgaa cgccttatcc agcacacatc tggcagc
//
LOCUS       DAFATA010000054        27761 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFATA010000054 DAFATA010000000
VERSION     DAFATA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.055"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(95..457)
                     /locus_tag="LGJ20_002779"
     CDS             complement(95..457)
                     /locus_tag="LGJ20_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBK2386814.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            994..2907
                     /gene="mtlA"
                     /locus_tag="LGJ20_002780"
     CDS             994..2907
                     /gene="mtlA"
                     /locus_tag="LGJ20_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK2386815.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3137..4285
                     /gene="mtlD"
                     /locus_tag="LGJ20_002781"
     CDS             3137..4285
                     /gene="mtlD"
                     /locus_tag="LGJ20_002781"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK2386816.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            4285..4872
                     /gene="mtlR"
                     /locus_tag="LGJ20_002782"
     CDS             4285..4872
                     /gene="mtlR"
                     /locus_tag="LGJ20_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBK2386817.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4884..5093)
                     /locus_tag="LGJ20_002783"
     CDS             complement(4884..5093)
                     /locus_tag="LGJ20_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386818.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5378..5740
                     /locus_tag="LGJ20_002784"
     CDS             5378..5740
                     /locus_tag="LGJ20_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBK2386819.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6284..6967
                     /locus_tag="LGJ20_002785"
     CDS             6284..6967
                     /locus_tag="LGJ20_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBK2386820.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            7011..11861
                     /gene="ehaG"
                     /locus_tag="LGJ20_002786"
     CDS             7011..11861
                     /gene="ehaG"
                     /locus_tag="LGJ20_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HBK2386821.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12229..13884
                     /gene="lldP"
                     /locus_tag="LGJ20_002787"
     CDS             12229..13884
                     /gene="lldP"
                     /locus_tag="LGJ20_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBK2386822.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13884..14660
                     /gene="lldR"
                     /locus_tag="LGJ20_002788"
     CDS             13884..14660
                     /gene="lldR"
                     /locus_tag="LGJ20_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBK2386823.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MTHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            14657..15847
                     /gene="lldD"
                     /locus_tag="LGJ20_002789"
     CDS             14657..15847
                     /gene="lldD"
                     /locus_tag="LGJ20_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBK2386824.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            16001..16474
                     /gene="trmL"
                     /locus_tag="LGJ20_002790"
     CDS             16001..16474
                     /gene="trmL"
                     /locus_tag="LGJ20_002790"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK2386825.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWVDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16527..17348)
                     /gene="cysE"
                     /locus_tag="LGJ20_002791"
     CDS             complement(16527..17348)
                     /gene="cysE"
                     /locus_tag="LGJ20_002791"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK2386826.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17428..18447)
                     /gene="gpsA"
                     /locus_tag="LGJ20_002792"
     CDS             complement(17428..18447)
                     /gene="gpsA"
                     /locus_tag="LGJ20_002792"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK2386827.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18447..18914)
                     /gene="secB"
                     /locus_tag="LGJ20_002793"
     CDS             complement(18447..18914)
                     /gene="secB"
                     /locus_tag="LGJ20_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBK2386828.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18977..19228)
                     /gene="grxC"
                     /locus_tag="LGJ20_002794"
     CDS             complement(18977..19228)
                     /gene="grxC"
                     /locus_tag="LGJ20_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBK2386829.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19369..19800)
                     /locus_tag="LGJ20_002795"
     CDS             complement(19369..19800)
                     /locus_tag="LGJ20_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK2386830.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGLQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20045..21589
                     /gene="gpmM"
                     /locus_tag="LGJ20_002796"
                     /gene_synonym="pgmI"
     CDS             20045..21589
                     /gene="gpmM"
                     /locus_tag="LGJ20_002796"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBK2386831.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21599..22882
                     /gene="envC"
                     /locus_tag="LGJ20_002797"
     CDS             21599..22882
                     /gene="envC"
                     /locus_tag="LGJ20_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBK2386832.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22886..23845
                     /locus_tag="LGJ20_002798"
     CDS             22886..23845
                     /locus_tag="LGJ20_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBK2386833.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREIATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(23832..24866)
                     /gene="waaH"
                     /locus_tag="LGJ20_002799"
     CDS             complement(23832..24866)
                     /gene="waaH"
                     /locus_tag="LGJ20_002799"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBK2386834.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(25105..26130)
                     /gene="tdh"
                     /locus_tag="LGJ20_002800"
     CDS             complement(25105..26130)
                     /gene="tdh"
                     /locus_tag="LGJ20_002800"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK2386835.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(26140..27336)
                     /gene="kbl"
                     /locus_tag="LGJ20_002801"
     CDS             complement(26140..27336)
                     /gene="kbl"
                     /locus_tag="LGJ20_002801"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK2386836.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            27550..>27761
                     /locus_tag="LGJ20_002802"
     CDS             27550..>27761
                     /locus_tag="LGJ20_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK2386837.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEA"
BASE COUNT         7045 a         7107 c         7109 g         6500 t
ORIGIN      
        1 gtcgccagcg tccactgatt taccggaatg cggaagattt taaagaccgc ccacgccagc
       61 gccacgtaag ttaaaacaat caatagatcc ataattagat ctccgggaac gtggaatagt
      121 cagtcttttt ctcggcggcg agttgatgct ccagccggga aattcggtca gaaagtgagg
      181 cgatttccgg gtcagttgct ttctcctgcg acgcaacatg tgactgcata ccccagccac
      241 gctccggttg atagagcgtc gcccagatcc acagaaacgg ccagataaca tgcagagtaa
      301 acaggctcac ccagcccgcc gtatgaatag cgtcggcatg ggggtgattg cgctttttgg
      361 caatcagata agggatgtca tgtatcgcga tgattccata aaaaatcacc agaaagacga
      421 agatcagcac tcccaacgcg aaatagttta gaaacatatc tgcctcggat tcacgtttat
      481 cagtgttgtt tttgggctgg cagccagaag ggagtcaggc tgatattttg acaataatcc
      541 gggttcgcga ttctcgccat aacaccaaag aataattttt agaggtgatg agttgcttag
      601 ttacataacg attgtatgac gaaggcataa catgctgtag atcacatcag gtgaacgccg
      661 taagaaaata tcttgtgatt cagatcacaa agattcaaca aaccatcaaa acaaaaatgt
      721 gacactactc acatttaaat gccattttta gcgaaaatcg ccgtcttgtt gcttttttac
      781 acaagcgttt tgtgatgagt atcacgtcaa ttacctctct accccctata tttatgtgac
      841 tgatatcaca caaaaggccg tcgactggac agttaaccga ttcagtgcca gatttcgcag
      901 tatctacaag gtccggctac ctctgccgcc acattaacaa aaaacctcgg gcttccagcc
      961 tgcgcgacag caaacataag aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg
     1021 caaagctttg gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg
     1081 tggggtatca tcaccgcgtt atttattcca acagggtggt taccgaacga gacgctggcg
     1141 aagctggtcg ggccgatgat cacttatctc ctgccgctgc tgatcggtta taccggtggt
     1201 aagctggtag gcggcgaacg tggcggcgta gtcggtgcca tcactaccat gggcgttatc
     1261 gtcggcgcag acatgccgat gttcctcggt tctatgattg caggtccgct gggcggctgg
     1321 tgcattaagc acttcgaccg ctgggtagac ggtaagatca aatccggttt tgagatgctg
     1381 gtgaataact tctccgcagg catcatcggg atgatcctcg ccattctggc atttctcggc
     1441 attggcccga ttgttgaagc cctgtccaaa atgctggctg cgggcgttaa cttcatggtt
     1501 gtccatgaca tgctgccgct ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc
     1561 aacaacgcca ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc ccatgaactg
     1621 ggtaaatcca tcttcttcct gattgaagct aacccaggtc caggtatggg cgtgctgctg
     1681 gcgtacatgt tctttggtcg tggtagtgct aaacagtctg cgggcggtgc ggcaatcatc
     1741 cacttcctgg gtggtatcca cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg
     1801 atcctggccg ttatcctcgg cggtatgact ggcgtgttca cgctgactat cctgggcggt
     1861 ggtctggttt ctccagcatc tccggggtct atccttgcgg tactggcgat gacgccaaaa
     1921 ggtgcttact tcgctaacat cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc
     1981 tctgctattt tgctgaaaac cagcaaagtg aaagaagaag atgatattga agcagcaact
     2041 cgtcgtatgc aggacatgaa agctgagtct aaaggcgcat ctccgctgtc tgctggcgat
     2101 gtgactaacg acctgagcca cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt
     2161 tccagtgcga tgggcgcagg cgttctgcgt aagaaaattc aggatgcagg tctgtcgcag
     2221 atttctgtta ctaacagcgc gatcaacaac ctgccgccag atgtggacct cgtcatcact
     2281 caccgtgacc tgaccgaacg cgctatgcgc caggttccgc aggcacagca tatttcgctg
     2341 accaacttcc tcgacagcgg cctgtacacc agcctgaccg aacgtctggt tgctgcccaa
     2401 cgccacacgg caaacgaaga gaaagtaaaa gacagcctga aagacagctt tgacgattcc
     2461 agtgctaacc tgttcaagct aggcgcggag aacatcttcc tcggtcgcaa agcggcaacc
     2521 aaagaagaag cgattcgttt tgctggcgag cagctggtga aaggcggtta cgttgagccg
     2581 gaatacgttc aggcgatgct ggatcgtgaa aaactgaccc cgacttatct gggtgagtct
     2641 atcgcggtgc cacacggtac ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg
     2701 ttctgccagt acccggaagg cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg
     2761 gtgattggta ttgcagcccg taacaacgag cacattcagg ttatcaccag cctgaccaat
     2821 gcactggatg atgagtccgt catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg
     2881 ctggaactgt tggcaggtcg taagtaatcc aatcccaccc tctccacatg gagaaggtgg
     2941 ggttaattgc ctgatgcgct acgcttatca ggcctacagg atgcatcacg atttgttgaa
     3001 tttgcacgtt cttgtaggcc ggataaggcg cttacgccgc atccggcgct gcccctctcc
     3061 tcacggagag ggtttgggtg agggaaaagc ctcaccccag ccctctcggg taaaaacatt
     3121 gatgaaggtt aatactatga aagcattaca ttttggcgca ggtaatatcg gtcgtggctt
     3181 tatcggtaaa ctgctggcag acgcgggtat ccaactgacg tttgccgatg tcaatcaggt
     3241 ggtacttgat gccctgaatg cccgtcatag ctatcaggtt catgtggttg gtgaaaccga
     3301 gcaggtagat accgtttccg gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga
     3361 tctgattgct caggtcgatt tagtcactac cgccgttggc ccggttgtgc tggaacgtat
     3421 tgctccggct atcgccaaag ggctggtgaa acgcaaagaa caaggtaatg aatccccgtt
     3481 gaacatcatc gcctgtgaaa acatggtacg cggtaccacg cagctgaaag gccatgtgat
     3541 gaacgccctg ccggaagacg ccaaagcgtg ggtagaagaa cacgttggct ttgtcgattc
     3601 cgccgttgac cgcatcgtac cgccttcggc ttcggcaact aacgatccgc tggaagtgac
     3661 ggtagaaact ttcagcgaat ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa
     3721 catcccaggc atggagttaa ccgacaacct gatggcattt gtcgaacgta aactcttcac
     3781 cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc atcagaccat
     3841 tcgtgacgct attctcgacg agaaaatccg cgcggtggta aaaggtgcga tggaagaaag
     3901 tggtgcggta ctgatcaagc gctacggctt tgacgcagac aaacatgcgg cgtacatcca
     3961 gaaaatcctc ggtcgttttg agaacccgta tctgaaagat gatgtagagc gcgtaggccg
     4021 tcagccgctg cgtaaactga gtgctggcga ccgtctgatc aagccattgc tcggtacgct
     4081 ggaatacagc cttccgcaca agaatctgat tcaggggatt gctggtgcaa tgcacttccg
     4141 cagtgaagat gatccgcagg ctcaggaact ggcagcactg atcgctgaca aaggtccgca
     4201 ggcggcgctg gcacagattt ccggtcttga tgccaacagc gaggttgtat ccgaggcggt
     4261 aaccgcttat aaagcaatgc aataatggtg gaccaggcgc aggacaccct gcgcccgaat
     4321 aacagattgt cagatatgca ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg
     4381 cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt
     4441 ttgaccgagg cggtaaatct tctggtgctt caggtattcc gcaaagacga ttacgcggtg
     4501 aagtatgctg tagaaccgtt actcgacggc gacggtccgc tgggcgatct ttctgtgcgt
     4561 ttaaaactca tttacgggtt gggcgtcatt aaccgccagg aatacgaaga tgcggagctg
     4621 ctgatggcat tgcgtgaaga actaaatcac gacggcaacg agtacgcctt taccgacgac
     4681 gagatccttg gaccctttgg tgaactgcac tgcgtggcgg cgttaccacc gccgccacag
     4741 tttgaaccag cagactccag tttgtatgca atgcaaattc agcgctatca acaggctgtg
     4801 cgatcaacaa tggtcctttc actgactgag ctgatttcca gaatcagctt aaaaaaagcc
     4861 tttcaaaagt aagcaacgtc tgcttactgc ccctctacct gcttcggccg ataaagccga
     4921 cgataatact ccagacgttg tagatataac ggcacgcttt catcgggtat tccggacgga
     4981 atcgcgttac ggggaggaag ttttttcaga tactcccgga acgcctggct tgatgccatg
     5041 aaatctacgg ctttatcgat aagaagcgga acgttttcac ctaatttgcc catgatgata
     5101 tctccgtatt accccgcgcc gggaatgcgc ggccgccaat tttagtttag gttcccggta
     5161 aagatgacat taaggaaaac gtgctgaatc ctcaataatc atccgctgct gtttattatc
     5221 ttttttctat agatttctta attaatcaat aaattgcgta gtttttaagc atgttaatga
     5281 ttacgttaca gaactattgc aatgtcatca gttgtgaaca aagcacctgg tcgcgcatac
     5341 taggggctat aaatttatct ttatcagaag ccatcacatg aaagaagtcg aaaaaaacga
     5401 aatcaaacgt ctcagcgatc gtctggacgc catccgccac cagcaggccg atctgtcgct
     5461 ggttgaagcc gcagacaaat atgccgagct ggaaaaagag aaagccacgc tggaagcaga
     5521 aattgctcgc ctgcgtgaag ttcatagcca aaaactgagt aaagaagcac agaagctgat
     5581 gaagatgccg tttcagcgcg caattaccaa aaaagagcag gctgatatgg gcaagctgaa
     5641 gaaaagtgtt cgcggactgg tggttgtgca cccaatgacc gcgctgggcc gtgaaatggg
     5701 cctgcaggag atgactggat tctctaagac tgcgttttaa gcgcctcaga cgactggttc
     5761 gatcatatca acccgcatct ggcttgcctt agctggtcaa tgcggggact ctcctgtcta
     5821 ctcgcctaat taccaaacaa ttcactccct ttacttcgat ttaccgctgg ttgatattct
     5881 gaaaatgcat ttttaccgta atagccatct ataaacaata aaaatatata gaagatatca
     5941 tttgttaatt aacaagcatt tacgtctctt tttttcggat ttaattgtga aattttaatc
     6001 atcatattca caaaatcaat tatctgtaaa atttttccca gacatggttt ctttcatatt
     6061 tgacatttat taataatcat cattagaatc cacgaaaaat aatatattca tacaaacaca
     6121 gacatctttt gaaacgaatg atacattttt atgatgaatg cttaaaacaa tatttaatcg
     6181 acacacatca ctgtcattaa tgtaaagcaa catagatcac caataataca atggacaact
     6241 ctaccggcat tgatcgctgg tcggaggaaa taaggatttt tacatgaact tgaagaagat
     6301 ttttttcagt gctgtcactg tttcagttct ctgcgcatta acgggatgtg actacattga
     6361 agaaggcaag ccagaaagta gtttattgaa acaacaagaa gaacataaca ataaaattga
     6421 tctacttgaa aaacaacaag cacagctaaa gagtcaactg gaaactattc aaaaacaaca
     6481 aactggcata ataagcagta caaagacttt aactcacgta attaaatccg ttaaagatca
     6541 acaaaacact tttattttta ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa
     6601 caatggttcg gtggctttag cgggtcgagt gttatccatt gacgccacag agaatggcag
     6661 tgttattcat atttcactgg tcaacttatt aagtacacct atctcaaata ttggttttaa
     6721 tgcgacatgg ggtggtgaaa aacctgtcga tgccaaagag tttgcccggt ggcaacaatt
     6781 acttttcaac acatcaatga aatccacatt gaaattatta ccaggtcaat ggcaagacat
     6841 taatttgacc ctgaagggtg tatcgcccaa taacctggga tatctgaagt tagccatcaa
     6901 catggaaaat attcagttcg acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa
     6961 aaaataaatg cagttagtac gcttataaca aggaatagcg agcatcatta atgaacaaaa
     7021 tatttaaagt tatctggaac cctgcgacag ggaattatac tgttaccagc gaaacggcaa
     7081 aaagccgtgg caagaaatct gggcgcagta agctgttaat ttctgcgctg gttgcgggtg
     7141 gaatgttgtc gtcgtttggg gcattggcga atgccgggaa tgacaacggt cagggtgttg
     7201 attacggtag tggatcagct ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa
     7261 atacttttat gaacaccagt ggttccagta ctgctgtggg ttatgacgct atagctgaag
     7321 gccaatatag ctctgccatc gggtcaaaaa cccatgcgat tggcggtgca tcaatggcct
     7381 ttggggttag tgcaatatca gaaggcgata gaagtatagc actgggtgcc tcttcgtatt
     7441 cattgggcca atactcaatg gccctcggcc gttattcaaa agcattgggt aaattgtcta
     7501 ttgctatggg ggactcttcc aaagcggaag gagcaaacgc cattgccctg ggaaatgcca
     7561 ctaaagctac tgagattatg agtattgctc ttggcgacac cgccaatgcg tcaaaagcgt
     7621 attcaatggc gctgggagca agtagcgtcg catctgaaga aaacgcaatt gccctggggc
     7681 gtagcagtgt agctagcggt actgacagcc tcgcatttgg cagacaatca cttgccagcg
     7741 cagcgaacgc tattgcgata ggtgctgaga ccgaagccgc tgaaaatgca actgctattg
     7801 gcaataatgc gaaggcaaaa gggactaata gcatggcaat ggggttcgga agccttgccg
     7861 ataaagtcaa tactatcgca ttaggaaatg gcagccaggc tctggcagat aatgcaatcg
     7921 ccataggcca gggcaacaaa gctgatggcg tggatgccat cgctctgggt aatggtagcc
     7981 agtcgagagg cttaaacacc attgccttag gcacagccag taatgcaact ggtgataaga
     8041 gtcttgcgct tggtagtaat agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag
     8101 attccattgc ggatttagac aataccgtct ctgtcggcaa tagttcatta aaacgcaaga
     8161 tcgttaatgt gaaaaatggc gcgatcaagt ctgacagtta cgatgccatt aatggttcac
     8221 agctttatgc cattagcgac tcggtagcaa aaaggcttgg aggaggggct gcagtagatg
     8281 ttgatgacgg tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg
     8341 taggggctgc gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca
     8401 aatacagcgc tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg
     8461 atggcacgat ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca
     8521 atgacgatgt cgaagccaat accgccaata tcgctactaa taccagcaac attgccacga
     8581 atacggcaaa tattgccacc aataccacca atatcaccaa cctgacggat tccgttggtg
     8641 accttcaggc tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg
     8701 gccaggatac caccagcaaa atcaccaacg ttaaagatgc cgacctgacg gctgacagca
     8761 ctgatgctgt taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca
     8821 ccaatatcgc caataacact tccaatattg ccactaacac caccaacatc tctaacctga
     8881 ctgagacggt gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat
     8941 tcacggcagc tcatggcacc gagaccacca gcaaaatcac caacgttaaa gatggcgacc
     9001 tgacgactgg cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg
     9061 tggcgacgaa taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga
     9121 cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg
     9181 cagctcatgg caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg
     9241 caaccagttc cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca
     9301 cctacttcgg cggcaatgct tctgtgaata ctgacggtgt gtttaccggt ccaacctaca
     9361 aaatcggtga aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat
     9421 ttagcacgtc tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca
     9481 aacacggtac taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag
     9541 actccagttc tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg
     9601 atgcgctggg aggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca
     9661 ccattgctaa tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc
     9721 ttgacgacgc tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc
     9781 acggaaaaga taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg
     9841 ccagcagcga cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg
     9901 cgctgggtgg tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca
     9961 ttgcgaacgc cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgcct
    10021 tgctgtggga tgagactgcc aatggcggtg ctggagccta caatgccagc catgacggta
    10081 aagccagcat catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag
    10141 tgaacggttc tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca
    10201 atcagctcgc tggtaacacc gacgcaacct atatccaaga aaacggtgcg ggtattaact
    10261 atgtgcgtac taacgacgac ggcttagcgt tcaacgacgc cagcgcacag ggtgttggcg
    10321 ctacagctat aggttataac tctgtcgcca aaggcgatag cagcgtagct attggtcagg
    10381 gcagctacag cgacgttgat acgggtatcg ccctgggtag cagctctgtt tccagccgag
    10441 tgattgcaaa aggctcccgt gacaccagca taacggaaaa tggcgttgtt attggttacg
    10501 acaccacgga tggcgaactg ctcggtgcat tgtctatcgg tgatgacggt aaatatcgtc
    10561 aaatcatcaa cgtagccgat ggttccgaag cccatgacgc cgttacggtt cgtcaattgc
    10621 agaatgcgat tggtgcggtc gcaaccacgc cgactaaata cttccacgct aattcaacgg
    10681 aagaagattc actggcagtg ggaactgact cgctggcaat gggtgcgaaa accatcgtga
    10741 atggcgataa aggtattggt atcggttatg gtgcctacgt ggacgcgaat gcacttaacg
    10801 gcattgccat tggtagcaat gcgcaagtca ttcatgtcaa cagtattgcg ataggtaatg
    10861 gttctacgac cactcgtggc gctcaaacca attataccgc ctacaacatg gacgcaccgc
    10921 agaactctgt cggtgaattc tcagtcggta gtgcggatgg tcaacgtcag atcactaacg
    10981 tcgcagcagg ttcggctgat accgatgcgg tcaacgtggg tcagttgaaa gtaacggatg
    11041 cgcaggtttc ccagaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg
    11101 atactcgcgt tactaatatc gaaaacggta ttggcgatat cgtcaccacc ggtagcacca
    11161 agtacttcaa gaccaatacc gatggtgtag atgccagcgc gcagggtaaa gatagcgtcg
    11221 cgattggttc tggctccatt gctgccgcgg ataacagcgt cgcactgggt acagggtctg
    11281 tggcaaccga agaaaatacg atctctgtag gctcatctac taatcaacgt cgtatcacca
    11341 acgtagccgc aggtaaaaac gacaccgatg ctgttaacgt ggcacagttg aagtcttccg
    11401 aagctggcgg tgtgcgttac gacaccaaag ctgatggttc tatcgactat agcaatatca
    11461 ccctcggtgg cggcaacggc ggtacgactc gtatcagcaa cgtctccgct ggcgtcaaca
    11521 acaacgacgc ggtgaattac gcgcagttga agcaaagcgt gcaggaaacg aagcaataca
    11581 ccgatcagcg aatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg
    11641 gtggtatcgc ttctgcaatg gcaatgaccg gtctgccgca ggcttacact ccaggtgcca
    11701 gcatggcctc tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat
    11761 cgatggtgag cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg
    11821 gtgaatactc cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt
    11881 gactaagggc aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct
    11941 ccgggtcaac aatctaaggg caattctctg atgaggattg cccttttctt taccagacat
    12001 ctccccccac aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt
    12061 ccccaattaa aactcacatc aatattgcca atacataaca tttagttaac cattcattgt
    12121 cattatccct acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat
    12181 gagcaacaga ctcattacac gatgtgcgtg gactccagga gacctgcaat gaatctctgg
    12241 caacaaaact acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc
    12301 atcctgtttt tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca
    12361 tggacggtag caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac
    12421 gcgctggcct cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt
    12481 attgcagcag tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc
    12541 tcgtctattc tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt
    12601 ttcggcgctt tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca
    12661 ttgctggtcg gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac
    12721 accgcgccag tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca
    12781 ggcatcgaca gctttgagat tggccagatg gtggggcggc agctaccgtt tatgaccatt
    12841 atcgtgctgt tctggatcat ggcgattatg gacggctggc gcggtatcaa ggagacgtgg
    12901 cctgcggtcg tggttgcggg cggctcgttt gccatcgctc agtaccttag ctctaacttc
    12961 attgggccgg agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg
    13021 ttcctcaaac gctggcagcc agtgcgcgta ttccgttttg gtgatttggg ggcgtcacag
    13081 gttgatatga cgctggccca caccggttac actgcgggtc aggtgttacg tgcctggaca
    13141 ccgttcctgt tcctgacagc taccgtaaca ctgtggagta tcccgccgtt taaagccctg
    13201 ttcgcatcgg gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat
    13261 aaactggttg cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt
    13321 aagtttgact ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt
    13381 gtctggctga agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa
    13441 ctggctctac ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat
    13501 tccggactgt catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc
    13561 ttctcgccgt tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac
    13621 gccctgttcg ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg
    13681 ctggttgccg ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc
    13741 gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact
    13801 gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat
    13861 gtcttaacgt ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg
    13921 ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg
    13981 ctgagcgcca actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg
    14041 caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct
    14101 ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg
    14161 atgatccgga ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg
    14221 catggcatgc ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg
    14281 aagcaacgct aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg
    14341 cgattgccga agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg
    14401 tcctgcaatc ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt
    14461 cacaactgac cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg
    14521 gggcgcgtaa agcaatgatg acgcacctta gttttgttca caccaccatg aaacgattcg
    14581 atgaagatca ggctcgccac gcacggatta cccgcctgcc cggtgagcat tatgagcatt
    14641 cgagggagaa aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg
    14701 cattctgccg ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct
    14761 gcgccgcaac gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat
    14821 gtctgactta agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact
    14881 ggcaccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc
    14941 ggcggacgcg catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga
    15001 agtcgcgcca gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg
    15061 ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac
    15121 cgtggatatg ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc
    15181 aaatgcggca atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt
    15241 gggcctgaac ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac
    15301 cggactggaa gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa
    15361 agaccttgag tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga
    15421 tccggaagat gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca
    15481 cggtggccgc cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga
    15541 tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt
    15601 cgtgcgtatg attgcgctcg gtgccgacac cgtacttctg ggtcgtgctt tcctgtatgc
    15661 gctggcaaca gcgggccagg cgggtgtagc taacctgcta aatctgatcg agaaagagat
    15721 gaaagtggca atgacgctga ctggcgcgaa atcgatcagc gaaattacgc aagattcgct
    15781 ggtgcagggg ctgggtaaag agttgcctgc ggcactggcc ccgatggcga aagggaatgc
    15841 ggcatagtcg tttgcgcccc ctccccctaa ccctctccct cagggagctg tctcttgatc
    15901 agatctcctg atcaagagac agggggaggc cggggtgagg ggaaaaggcc gcactgctca
    15961 ccaattttct gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta
    16021 cgaaccagaa attccgccaa atactggcaa catcatccgt ctttgcgcta ataccggctt
    16081 tcgtctgcat atcatcgaac cgatgggatt tgcctgggtc gataagcgcc tgcgccgcgc
    16141 ggggctggac tatcacgagt ttaccgccgt tacgcgtcat catgactttc gcgcgttcct
    16201 cgaagcagaa aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag
    16261 cgccgtaagc tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctacc
    16321 agcgagcatt cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga
    16381 cagccgcagc atgaacctgt ccaatgcggt gtcagttgtg gtgtatgaag cctggcggca
    16441 gctgggatat ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac
    16501 atcgcatccg gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta
    16561 ataccgttga aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca
    16621 atacgagccg gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa
    16681 cctgcgccaa tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg
    16741 ccaatcatca caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg
    16801 ccaagcgtca cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca
    16861 acgatgcccg tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata
    16921 tcgacctgga acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc
    16981 tgattccaca accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat
    17041 aacaacgggg ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca
    17101 caggccgcag aggcgatcat ttccgggtca gcggcgtagg cttcttccac cacttcacgg
    17161 atagcaatag caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca
    17221 cttccaaggt tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca
    17281 cagtccgcca gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca
    17341 cacgacattg cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt
    17401 catagcggta acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg
    17461 acctaataac gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat
    17521 ttcctcggtt attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt
    17581 attgcggtag ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc
    17641 ctgaccgagc atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac
    17701 cagatcgcca agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcacc
    17761 aagacgcgac atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa
    17821 accgataccg tcggacatcc ccgcgccaat ggcaatgacg tttttcaccg caccgccaag
    17881 ctgcacaccg atgaaatccg ggttgctgta aacgcggaaa cttttgccac agtgcagcaa
    17941 ttgttggaga tcatcggcaa aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa
    18001 acctgccgcc agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc
    18061 gcctaaggcc tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt
    18121 ggtcgcccac accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc
    18181 accaaagaca tggctgggta cgacgacgag aatattacgg ctggctgcca gcgcagtggc
    18241 gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt
    18301 acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc
    18361 gtggccattt cttgccaggg tgatggcaag agcggtgccg tacgagccgg caccgatcac
    18421 agtcattgaa gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca
    18481 gcctgctgct gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt
    18541 tgcgggaatg taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga
    18601 atgttcgggc agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg
    18661 gagaaaatac cgccctgctg aacttcacac aggaacgcgg tttcttcgcc cagggaggcc
    18721 gttacggtaa cacgcagtac cacttcgtaa acgtcatctg ccagttgagt agatgccgta
    18781 tccagatcaa gtttaacttc tggctgccaa tctttctgga aaacgtgcgg cgcgttcggc
    18841 gcttcgaaag aaatatcctt ggtataaata cgttggatct ggaaagtcat ttcggtgttg
    18901 ttttgttctg acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa
    18961 tgccaacgtt cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat
    19021 acaagtcatc acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc
    19081 gaccgctgcg tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct
    19141 ggaaactcac gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt
    19201 ctttagtata gatttcaaca ttggccataa attagctcct gttttttacc ctgaaatttc
    19261 atgttgcagg gcggcagcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg
    19321 gggcctaatt gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg
    19381 caccaaaggc aagttttcgc cagcccagcc agcgacgccc tctttcagca cgaatacttg
    19441 cgcaaaacca gctttcgtca gcgcatttgc aggctcctgg cactgcaggc cagaaccgtc
    19501 taccacgata accggtttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat
    19561 ttcgctcggc aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg
    19621 acgtaaatcc acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgact
    19681 aatcaccttc actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa
    19741 cgcgatccag gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat
    19801 ggggggtaac aactcccgac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg
    19861 gtgcggcaaa tacagccagc gcgttaactg gaatgcaatt ttgcggggcg cgacgaaaaa
    19921 aaagcgtaaa tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct
    19981 attttatcga ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt
    20041 cgcaatgtcg gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg
    20101 cgaagaacag caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg
    20161 ggccaatcgt ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg
    20221 tcagatgggt aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca
    20281 ggacctgact cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct
    20341 gactggcgcg gtagataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct
    20401 ctccgcaggc ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc
    20461 tgaacgcggc gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc
    20521 gcgcagtgct gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg
    20581 ccgcgtagcg tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg
    20641 cgttgaaaaa gcctatgacc tgctgactct ggcgcagggt gagttccagg ccgatactgc
    20701 cgttgctggt ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac
    20761 cgttattcgt gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt
    20821 catgaacttc cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt
    20881 cgatggcttc gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc
    20941 cgctgacatc aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga
    21001 gtggatggcg aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca
    21061 cgttactttc ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct
    21121 gatcaactca ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct
    21181 gaccgaaaaa ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta
    21241 tccgaacggc gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga
    21301 agcactggat cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact
    21361 gctgatcacc gctgaccacg gtaacgctga acagatgcgc gatccggcaa cgggtcaggc
    21421 acacacggca cacaccaacc tgccagttcc gctgatttat gttggtgata agaacgtgaa
    21481 agcggttgaa ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat
    21541 ggaaatcccg caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat
    21601 gaggggaaag gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag
    21661 gcctatcatc tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca
    21721 cgcggatgag cgtgaccaac tcaaatctat tcaggccgat atcgccgcaa aagagcgcgc
    21781 ggtacgccaa aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga
    21841 agcgatctct gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa
    21901 taaacagatt gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca
    21961 ggagcgcagc ctcgccgcgc aactggatgc cgcattccgt cagggcgagc ataccggtat
    22021 tcagctaatt ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg
    22081 ctatctcaac caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt
    22141 cgccatgcag cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga
    22201 acagcgcgcc caacaggcga aactgactca ggctctgaac gagcgtaaaa agacgctggc
    22261 ggggctggaa tcttccatcc agcaaggtca gcagcagcta agtgaactgc gtgccaacga
    22321 atcccgtctg cgtaacagca ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg
    22381 agaagcgcgt gaggcccagg cggttcgcga ccgccagaaa gaagcgacgc gcaaaggcac
    22441 cacctataaa ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc
    22501 gccgcgtggt caggcattct ggccggttcg cgggccgacg ctgcatcgct atggtgaaca
    22561 gctacagggc gaactacgct ggaaaggaat ggttatcggt gcctctgaag gtactgaagt
    22621 taaagcgatt gccgacggtc gggtgattct ggctgactgg ctgcaaggtt acggtctggt
    22681 ggtggtggtt gagcacggta aaggcgacat gagtctttac ggctataatc agagcgcact
    22741 ggtgagcgtt ggttcgcagg ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg
    22801 cggtcagggt cggccttcac tctatttcga aattcgccgc cagggtcagg cggtcaatcc
    22861 acagccgtgg ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc
    22921 cgctctgttg gcgctctcct cccccgtact tgctggcaaa cttgccatcg tcattgatga
    22981 ttttgggtat cgcccgcaca acgaaaacca ggtgctggcg atgccctccg ctatctccgt
    23041 cgctgtatta cccgattcac cgcacgccag agagatagcg accaaagcgc ataacagcgg
    23101 gcacgaagtg ttgattcatc tcccgatggc accactgagt aaacagccgc tggagaaaaa
    23161 tacgctgcgc ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa
    23221 taacgtgccc tatgccgtgg ggatcaacaa ccacatgggt agcaagatga cctctaacct
    23281 gtttggtatg cagaaagtga tgcaggcact ggagcgttac aatctttact tcctcgacag
    23341 cgtaaccatc ggtaataccc aggcgatgcg cgccgcgcaa ggtactggcg tgaaggtgat
    23401 caaacggaag gtgttcctcg acgattcgca gaacgaagcg gacatccgtg tgcaatttaa
    23461 tcgcgcaatt gatctggcgc gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc
    23521 ttcaacggta cgcgtgctac aacagatggt ttataacctg ccgccagaca ttacgctggt
    23581 gaaagccagc agcttgctga atgaaccgca ggttgatact tctacaccgc cgaaaaacgc
    23641 tgtgcctgat gcaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc
    23701 gatagaacct gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag
    23761 cacgctgatc gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc ccgaagcggc
    23821 gaagtttaac gctagcgcaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt
    23881 ttgtcgcgcc attgccataa gcggaaagac cacagtaacg cctggtaacc gaccttcacg
    23941 ctgcgcacat tggtaatcag gcgcttatac ataccggaag taaatatctc ggcaatcatc
    24001 cgttggcggg taagaatatc cggctctttg cgcaccgcat ggcaaacacg caatgcttcg
    24061 taagttattt gctgatgaaa ttccggataa atcgtaattt tgtcggcata atttcgattt
    24121 aatttctcca gcaggcgggt aatcttaata tagtgacgtt gataattaag gtttttattc
    24181 ccttgtctat gcaaccgact cactgacgta ttatgcagat aatatttata taatgattgc
    24241 tcggtatatc gcgcacgcag cgcgttaaac atgaattctg ttgtccagac aatatcctga
    24301 tgatgtaatc cggcaataaa tttaatgtta tttttaacaa taacatcacg gcgataaacc
    24361 cccatccaga caacgtgagt ccaacggcgc gaagaaagcc ccatccgcag ccagtccggg
    24421 ccggttaata cgccggttga gcgaaggcga tcgctgggga tggattgcca ggtttctccc
    24481 gtttcacgaa aacaccagtc agcgttgcac tgcgccacgt cgaggtcatc ctctaacgcc
    24541 atggtcatca gcgtttcgta catggtggga tagacctcat cgtcagcatc gacaaaagcg
    24601 acatatttgc ccgtcgccac ctcgatccca cgattacgcg ctaccgatgc gccagcattc
    24661 gcctgatgca acaaacgaac gtgcggatag ttttctgcgt aatgctttgc tatttcaaca
    24721 gaattatccg ttgaaccatc gttaataata atgatttcca gagcagtcca ggtttgcgta
    24781 attaaagatt ccatacaagt gcggaaatca tcgcccgcat tatataacgg aataataaca
    24841 ctaagtttat tggtgctgtt catcataaat taccgacgac ctctgaagtt atcctgacgt
    24901 tttacaggct ttgtattaag aaactattaa gcctgtgaga tattattttt acaggtgaga
    24961 gagtcacttc agggtagaga gataatgaga gcagctatat aagagatcag cgcaattacg
    25021 atatctttat tacactgaca ggattttaca tatttaacgg attatcctca ggcaaaaggg
    25081 ctggaatacc agcccttgtt cgtgttaatc ccaactcaga attactttcc cggactggcc
    25141 cgaacgcata gcgtcaaagc ccttctggaa atcatcaata gagaaacgat gggtaatgat
    25201 cggcgagaga tccagacctg actgaatcag cgccgccatc ttgtaccagg tttcaaacat
    25261 ctcacgaccg taaatacctt taatgaacaa gcctttaaag attactttgg tccagtcgat
    25321 agacatatcg gacggtggaa tacccagcat cgcaatgcga ccgccgtggt tcatggtgtc
    25381 gagcatggta cgaaacgctg gcggcgcacc ggacatttcc agaccgacat caaagccttc
    25441 ggtcatgccc agttctgcca tcacatcatt aaggttttct ttagcgacgt tgaccgcacg
    25501 ggtgataccc attttgcgcg ccagctcaag gcggtattcg ttaacatcgg tgatcaccac
    25561 attgcgtgca ccaacgtgtt tcgccaccgc cgcagccata atacctatcg gacctgcacc
    25621 agaaaccagc acatcttcgc ccaccagatc gaacgacagc gcggtatgca cggcgttacc
    25681 gaagggatca aaaatggaag ccaggtcgtc ggagatattg tcggggattt tgaaagcgtt
    25741 gaacgccgga atcaccagat attcggcgaa gcagcccgga cggttaacac cgacaccaat
    25801 ggtgttgcgg cacagatggg tacgaccgcc acgacagttg cgacagtgac cgcaggtgat
    25861 atggccttcg ccagaaacgc gatcgccgat tttgaagcct ttcacttcct gaccaatacc
    25921 taccacttca ccgacatatt catggcctac gaccatcggc accgggatgg ttttttgcga
    25981 ccactcatcc cagttataga tgtgaacgtc agtcccgcag atggctgttt tacggatttt
    26041 aatcagcaga tcgttatgcc cgagttccgg tacaggaacg tcggtcatcc agatgccctc
    26101 ttccgctttc agtttggata acgctttcat ctcacatcct caggcgataa cgcccagttg
    26161 tttaccaata cgcgtgaatg cttctactgc acgcgtaatt tgctcagggg tatgcgccgc
    26221 agacatctgg gtacgaatac gcgcctgacc tttcggaacg accggataga agaaaccggt
    26281 gacgtaaatg ccctcttttt gcagctcacg ggcaaatttc tgcgccacta ctgcatcgcc
    26341 aagcatgacc ggaataatgg catgatcggc tcccgccagg gtaaagcccg ccgccgacat
    26401 ttgctcacgg aactggcgcg cgttcgccca cagacggtca cgcagttcgc tgcccgcttc
    26461 gaccatctcc agtactttga tggacgcggc aacaatggcc ggtgccagcg agttggagaa
    26521 caggtacgga cgagaacgct ggcgcagcca ctcaaccacc tctttgcgcg ccgcggtata
    26581 accaccagaa gccccgccca gcgctttacc aagcgtaccg gtgataatat cgacccggcc
    26641 catcacatcg cagtattcat gggaaccacg accattttca ccgacaaaac cgaccgcgtg
    26701 ggagtcatcc accatcacca gggcatcgta tttatctgcc agatcgcaaa cgcctttcag
    26761 gttggcaatc acgccgtcca ttgagaacac accatcggtg gcaatcagca catgacgcgc
    26821 accggcttca cgcgcttctt tcagacgtgc ttccagctcc tgcatatcgt tgttggcata
    26881 gcgatagcgt ttagctttgc acagacgcac accatcaata atagacgcgt ggttcagtgc
    26941 gtcggagata atggcgtctt ccgcacccag cagcgtttca aacagtccac cgttagcatc
    27001 aaagcaggaa gagtagagaa tcgcatcttc catccccagg aatgccgcca gtttttgttc
    27061 aagctcttta tggctgtcct gagtgccgca aataaaacgc accgaagcca tgccgaaacc
    27121 gtgagaatcc attcccgcct ttgctgccgc aatcagatcc ggatgattcg ccagcccgag
    27181 atagttgttg gcacaaaagt taatgacgtg gcttccatca gccacagtga tatctgcttg
    27241 ctgcgcagac gtaataatgc gctcttcttt aaacaaccct tccgcccgtg cggtttccag
    27301 atcgttggtt aactgctgat aaaattctcc acgcattgcg attctccaga cagggcaaat
    27361 tccagcacat attacccaaa cttataggtc gcgacgagat gacgcgttaa cacttctgca
    27421 aaattcagga taaataacgt ccatcaatct cacttttggc gaatggctga agccaacgcc
    27481 gtgtagtgat atgatagaag tattcgtgtc tgagattgtc tctgactcca taattcgaag
    27541 gttacagtta tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag
    27601 gccctgaatg ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc
    27661 aagtttgtga acctggtgga tctggatatc gcggactata tggataagga agacttcctg
    27721 atccagatta tggctggcga agagttcggc gatgtcgaag c
//
LOCUS       DAFATA010000055        27572 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFATA010000055 DAFATA010000000
VERSION     DAFATA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.056"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            77..2611
                     /gene="mrcB"
                     /locus_tag="LGJ20_002803"
     CDS             77..2611
                     /gene="mrcB"
                     /locus_tag="LGJ20_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBK2386838.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2831..5074
                     /gene="fhuA"
                     /locus_tag="LGJ20_002804"
     CDS             2831..5074
                     /gene="fhuA"
                     /locus_tag="LGJ20_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBK2386839.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5125..5922
                     /gene="fhuC"
                     /locus_tag="LGJ20_002805"
     CDS             5125..5922
                     /gene="fhuC"
                     /locus_tag="LGJ20_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBK2386840.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5922..6812
                     /gene="fhuD"
                     /locus_tag="LGJ20_002806"
     CDS             5922..6812
                     /gene="fhuD"
                     /locus_tag="LGJ20_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBK2386841.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6809..8791
                     /gene="fhuB"
                     /locus_tag="LGJ20_002807"
     CDS             6809..8791
                     /gene="fhuB"
                     /locus_tag="LGJ20_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBK2386842.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDCVATERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8826..10106)
                     /gene="hemL"
                     /locus_tag="LGJ20_002808"
     CDS             complement(8826..10106)
                     /gene="hemL"
                     /locus_tag="LGJ20_002808"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBK2386843.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10331..11752
                     /gene="clcA"
                     /locus_tag="LGJ20_002809"
     CDS             10331..11752
                     /gene="clcA"
                     /locus_tag="LGJ20_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBK2386844.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGLTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11776..11841
                     /locus_tag="LGJ20_002810"
     CDS             11776..11841
                     /locus_tag="LGJ20_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386845.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11834..12178
                     /gene="erpA"
                     /locus_tag="LGJ20_002811"
     CDS             11834..12178
                     /gene="erpA"
                     /locus_tag="LGJ20_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBK2386846.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12225..12848)
                     /locus_tag="LGJ20_002812"
     CDS             complement(12225..12848)
                     /locus_tag="LGJ20_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBK2386847.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12886..13686)
                     /gene="btuF"
                     /locus_tag="LGJ20_002813"
     CDS             complement(12886..13686)
                     /gene="btuF"
                     /locus_tag="LGJ20_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBK2386848.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13679..14377)
                     /gene="mtnN"
                     /locus_tag="LGJ20_002814"
     CDS             complement(13679..14377)
                     /gene="mtnN"
                     /locus_tag="LGJ20_002814"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK2386849.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14461..15978
                     /gene="dgt"
                     /locus_tag="LGJ20_002815"
     CDS             14461..15978
                     /gene="dgt"
                     /locus_tag="LGJ20_002815"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBK2386850.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16108..17532
                     /gene="degP"
                     /locus_tag="LGJ20_002816"
     CDS             16108..17532
                     /gene="degP"
                     /locus_tag="LGJ20_002816"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBK2386851.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17687..18844
                     /gene="cdaR"
                     /locus_tag="LGJ20_002817"
     CDS             17687..18844
                     /gene="cdaR"
                     /locus_tag="LGJ20_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBK2386852.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18933..19319)
                     /locus_tag="LGJ20_002818"
     CDS             complement(18933..19319)
                     /locus_tag="LGJ20_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBK2386853.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19634..20458)
                     /gene="dapD"
                     /locus_tag="LGJ20_002819"
     CDS             complement(19634..20458)
                     /gene="dapD"
                     /locus_tag="LGJ20_002819"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBK2386854.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20489..23161)
                     /gene="glnD"
                     /locus_tag="LGJ20_002820"
     CDS             complement(20489..23161)
                     /gene="glnD"
                     /locus_tag="LGJ20_002820"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBK2386855.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23223..24017)
                     /gene="map"
                     /locus_tag="LGJ20_002821"
     CDS             complement(23223..24017)
                     /gene="map"
                     /locus_tag="LGJ20_002821"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK2386856.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24385..25110
                     /gene="rpsB"
                     /locus_tag="LGJ20_002822"
     CDS             24385..25110
                     /gene="rpsB"
                     /locus_tag="LGJ20_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK2386857.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25368..26219
                     /gene="tsf"
                     /locus_tag="LGJ20_002823"
     CDS             25368..26219
                     /gene="tsf"
                     /locus_tag="LGJ20_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK2386858.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26366..27091
                     /gene="pyrH"
                     /locus_tag="LGJ20_002824"
     CDS             26366..27091
                     /gene="pyrH"
                     /locus_tag="LGJ20_002824"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK2386859.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27382..>27572
                     /locus_tag="LGJ20_002825"
     CDS             27382..>27572
                     /locus_tag="LGJ20_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK2386860.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRT"
BASE COUNT         6441 a         6864 c         7593 g         6674 t
ORIGIN      
        1 agagatatct tcttctgtct tgtaacagaa gaacagaaaa tcgggctttt gcgcctgaat
       61 attgcggaga aaaagcatgg ccgggaatga ccgcgagcca attggacgca aagggaaacc
      121 gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac gaagatgacg atgattacga
      181 cgattatgat gactatgagg atgaagaacc gatgccgcgc aaaggtaagg gcaaaggcaa
      241 agggcgtaag cctcgtggca aacgcggctg gctatggcta ctgctaaaac tggctatcgt
      301 ttttgccgtg ctgatcgcca tttacggcgt ttatctcgat caaaaaattc gtagccgtat
      361 tgatggcaag gtctggcaac tgcctgcggc agtttatggc cgaatggtca atcttgagcc
      421 agacatgacc atcagcaaga acgagatggt gaagctgctg gaggcgaccc agtatcgtca
      481 ggtgtcgaaa atgacccgtc ctggcgaatt taccgtgcag gccaacagca ttgagatgat
      541 tcgccgtccg tttgatttcc cggacagtaa agaaggacag gtgcgcgcgc gtctgacctt
      601 tgatggcgat catctggcga cgatcgtcaa tatggagaac aaccgtcagt tcggtttctt
      661 ccgtcttgat ccgcgtctga tcaccatgat ctcttcgcca aacggtgagc agcgtctgtt
      721 tgtgccgcgc agtggtttcc cggatttgct ggtggatact ttgctggcga cagaagaccg
      781 tcatttttac gagcatgatg gaatcagtct ctactcaatc ggacgtgcgg tgctggcaaa
      841 cctgaccgcc ggacgcacgg tacagggtgc gagtacgctg acgcaacagc tggtgaaaaa
      901 cctgttcctc tccagcgagc gttcttactg gcgtaaagcg aacgaagctt acatggcgct
      961 gatcatggac gcgcgttaca gcaaagaccg tattcttgag ctgtatatga acgaggtgta
     1021 tctcggtcag agcggcgaca acgaaatccg cggcttcccg ctggcaagct tgtattactt
     1081 tggtcgcccg gtagaagagc taagcctcga ccagcaggcg ctgttagtcg gtatggtgaa
     1141 aggggcgtcc atctacaacc cgtggcgtaa cccaaaactg gcgctggagc gacgtaatct
     1201 ggtgctgcgt ctgctgcaac agcaacagat tattgatcaa gaactctatg acatgttgag
     1261 tgcacgtccg ctgggtgttc agccacgcgg tggggtgatc tctccacagc cagcctttat
     1321 gcaactggtg cgtcaggagc tgcaggcgaa attaggcgat aaggtaaaag atctctccgg
     1381 cgtgaagatc ttcactacct ttgactcggt ggcccaggac gcggcagaaa aagccgccgt
     1441 ggaaggcatt ccggcactga agaaacagcg taagttgagc gatcttgaaa ctgcgattgt
     1501 ggtcgtcgac cgctttagtg gtgaagttcg tgcgatggtc ggtggttctg agccgcagtt
     1561 tgcgggctac aaccgtgcga tgcaggcgcg tcgttcgatt ggttcccttg caaaaccagc
     1621 gacttatctg actgccttaa gccagccgaa aatctatcgt ctgaatacgt ggattgcgga
     1681 tgcgccaatc gcgctgcgtc agccgaatgg ccaggtctgg tcaccgcaga atgatgaccg
     1741 tcgttatagc gaaagcggca gagtgatgct ggtggatgcg ttgacccgtt cgatgaacgt
     1801 gccgacggta aatctgggga tggcgctggg gctgcctgcg gttacggaga cctggatcaa
     1861 actgggcgta ccgaaagatc agttgcatcc ggttccggcg atgctgctgg gggcgttgaa
     1921 cttaacgcca atcgaagtgg cgcaggcgtt ccagaccatc gccagcggcg gtaaccgtgc
     1981 accgctttct gcactgcgtt cggtaattgc ggaagatggc aaggtgctgt atcagagctt
     2041 cccacaggcg gaacgcgctg ttccggcgca ggcggcgtat ctgacactat ggaccatgca
     2101 gcaggtggta caacgcggta cgggtcgtca gcttggggcg aaatacccga acctgcatct
     2161 ggcagggaaa acagggacta ccaacaataa cgtagatacc tggtttgcag gcattgacgg
     2221 cagcacggtg accatcacct gggtaggccg tgataacaac cagccgacca aactgtatgg
     2281 agccagcggg gcaatgtcga tttatcagcg ttatctggct aaccagacgc caacgccgct
     2341 gaatcttgtt ccgccagaag atattgcaga tatgggcgtg gactacgacg gcaactttgt
     2401 ttgcagcggt ggcatgcgtg tcttgccggt ctggaccagc gatccgcaat cgctgtgcca
     2461 gcagagcgag atgcagcagc agccgtcagg caatccgttt gatcagtctt ctcagccgca
     2521 gcaacagccg caacagcaac ctgctcagca agagcagaaa gacagcgacg gtgtagccgg
     2581 ttggatcaag gatatgtttg gtagtaatta acatctaagc gtgaaatacc ggatggcgag
     2641 ttgccatccg gtaaaataac atcccatcta agatattaac cctttctttt catctggttg
     2701 tttattaacc cttcaggaac gctcagattg cgtaccgctt gcgaacccgc cagcgtttcg
     2761 aatattatct tatctttata ataatcattc tcgtttacgt tatcattcac tttacatcag
     2821 agatatacca atggcgcgtt ccaaaactgc tcagccaaaa cactcactgc gtaaaatcgc
     2881 agttgtagta gccacagcgg taagcggcat gtctgtttat gcacaggcag cggttgaacc
     2941 gaaagaagac actatcaccg ttaccgctgc acctgcgccg caagaaagcg catgggggcc
     3001 tgctgcaact attgcggcgc gacagtctgc taccggcact aaaaccgata cgccgattca
     3061 aaaagtgcca cagtctattt ctgttgtgac cgccgaagag atggcgctgc atcagccgaa
     3121 gtcggtaaaa gaagcgctta gctacacgcc gggtgtctct gttggtacgc gtggcgcatc
     3181 caacacctat gaccacctga tcattcgcgg ctttgcggca gaaggccaaa gccagaataa
     3241 ctatctgaat ggcctgaagt tgcagggcaa cttctataac gatgcggtca ttgacccgta
     3301 tatgctggaa cgcgctgaaa ttatgcgtgg cccggtttcc gtgctttacg gtaaaagcag
     3361 tcctggcggc ctgttgaata tggtcagcaa gcgtccgacc accgaaccgc tgaaagaagt
     3421 tcagtttaaa gccggtactg acagcctgtt ccagactggt tttgacttta gcgattcgtt
     3481 ggatgatgac ggtgtttact cttatcgcct gaccggtctt gcgcgttctg ccaatgccca
     3541 gcagaaaggg tcagaagagc agcgttatgc tattgcaccg gcgttcacct ggcgtccgga
     3601 tgataaaacc aatttcacct tcctttctta cttccagaac gagccggaaa ccggttatta
     3661 cggctggttg ccgaaagagg gaaccgttga gccgctgccg aacggtaagc gtctgccgac
     3721 agactttaat gaaggggcga agaacaacac ctattctcgt aatgagaaga tggtcggcta
     3781 cagcttcgat cacgaattta acgacacctt tactgtgcgt cagaacctgc gctttgctga
     3841 aaacaaaacc tcgcaaaaca gcgtttatgg ttacggcgtc tgctccgatc cggcgaatgc
     3901 ttacagcaaa cagtgtgcgg cattagcgcc agcggataaa ggccattatc tggcacgtaa
     3961 atacgtcgtt gatgatgaga agctgcaaaa cttctccgtt gatacccagt tgcagagcaa
     4021 gtttgccact ggcgatatcg accacaccct gctgaccggt gtcgacttta tgcgtatgcg
     4081 taatgacatc aacgcctggt ttggttacga cgactctgtg ccactgctca atctgtacaa
     4141 tccggtgaat accgatttcg acttcaatgc caaagatccg gcaaactccg gcccttaccg
     4201 cattctgaat aagcagaaac aaacgggcgt ttatgttcag gatcaggcgc agtgggataa
     4261 agtgctggtc accctgggcg gtcgttatga ctgggcagat caagaatctc ttaaccgcgt
     4321 tgctgggacg accgataaac gtgatgacaa acagtttacc tggcgtggtg gtgttaacta
     4381 cctgtttgat aatggtgtaa caccttactt cagctatagc gaatcgtttg aaccttcttc
     4441 gcaagttggg aaggatggta atattttcgc accgtctaaa ggtaagcagt atgaagtcgg
     4501 cgtgaaatat gtaccggaag atcgtccgat tgtagttact ggtgccgtgt ataatctcac
     4561 taaaaccaac aacctgatgg cggaccctga gggttccttc ttctcggttg aaggtggcga
     4621 gatccgcgca cgtggcgtag aaatcgaagc gaaagcggcg ctgtcggcga gtgttaacgt
     4681 agtcggttct tatacttaca ccgatgcgga atacaccacc gatactacct ataaaggcaa
     4741 tacgcctgca caggtgccaa aacacatggc ttcgctgtgg gctgactata ccttctttga
     4801 cggtccgctt tcaggtctga cgctgggcac cggtggtcgt tatactggct ccagctatgg
     4861 tgatccggct aactccttta aagtgggaag ttatacggtc gtggatgcgt tagtgcgtta
     4921 tgatctggcg cgagtcggca tggcgggctc caacgtggcg ctgcatgtta acaacctgtt
     4981 cgatcgtgaa tacgtcgcca gctgctttaa cacttatggc tgcttctggg gcgcagaacg
     5041 tcaggtcgtt gcaaccgcaa ccttccgttt ctaatttctc ttttggggca cggatttccg
     5101 tgcccatttc acaagttggc tgttatgcag gaatacacga atcattccga taccactttt
     5161 gcactgcgta atatctcctt tcgtgtgccc gggcgcacgc ttttgcatcc gctgtcgtta
     5221 acctttcctg ccgggaaagt gaccggtctg attggtcaca acggttctgg taaatccact
     5281 ctgctcaaaa tgcttggccg tcatcagcct ccgtcagaag gggagattct tcttgatgcc
     5341 cagccgctgg aaagctggag cagcaaagcg tttgcccgca aagtggctta tttgccgcag
     5401 cagcttcctc cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg
     5461 tggcatggcg cgttggggcg ctttggggcg gcagatcgcg aaaaagtcga ggaagctatc
     5521 tcgctggttg gcttaaaacc gctggcgcat cggctggtcg atagtctctc tggcggcgaa
     5581 cgtcaacggg cgtggatcgc catgctggtg gcgcaggata gctgttgtct gttgctcgac
     5641 gaaccgactt cggcgctgga tatcgcccac caggttgatg tgctggcgct ggtgcaccgt
     5701 ttaagtcagg agcgtggcct gacggtcatt gccgtgttgc acgatatcaa tatggcggca
     5761 cgctactgtg attatctggt cgccctgcgc ggtggtgaaa tgattgctca gggaacgcct
     5821 gcggaaatta tgcgcggcga aactctcgaa atgatttatg gcatcccgat gggtattttg
     5881 ccgcatccgg cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta
     5941 tttcgcgccg tcgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata
     6001 ccgcccacgc ggcggctatt gaccccaatc gtattgtggc gctggagtgg ttgccggtgg
     6061 aattactgct ggcgctcggc atcgtgcctt acggcgtggc ggataccatc aactatcgcc
     6121 tgtgggtcag cgaaccacca ttgccggact cagtgattga cgtcggtttg cgtacagaac
     6181 ctaaccttga actgctgacc gaaatgaaac cgtcgtttat ggtctggtcg gcaggatatg
     6241 gcccttcatc agaaatgctg gcacgtattg cgccgggtcg cggatttaac ttcagcgatg
     6301 gcaaacagcc gttggcgatg gcgcgtaaat cgctgacgga aatggcagat ttacttaacc
     6361 tgcaaagcgc agcggaaacg catttagcgc aatacgaaga ctttatccgc agcatgaaac
     6421 cccgctttgt gaagcgtggt gcacgcccgt tactgctgac gacgcttatc gatccgcgcc
     6481 atatgctggt cttcggtcca aacagcttgt tccaggaaat tctcgatgag tacggcatcc
     6541 caaatgcctg gcaaggggaa accaacttct ggggcagtac cgccgtcagt atcgatcgtc
     6601 tggcggcgta taaagacgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg
     6661 atgcgctaat ggcaaccccg ctgtggcagg ccatgccgtt tgtccgcgcc ggacgctttc
     6721 agcgcgtacc cgcagtctgg ttttatggtg cgacgctctc ggcaatgcac tttgtgcgcg
     6781 ttctggataa cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt ttccggcgtt
     6841 attgctggcg ctgctggtga ttgtcgctac ggcgctgacc tggatgaact tttcgcaggc
     6901 gctgccgcgc agtcagtggg cgcaggccgc ctggtcgccg gatattaacg tcatcgagca
     6961 gatgattttt cactacagct tgttaccacg tctggcgatt tctctactgg tgggcgcggg
     7021 cctggggctg gtgggcgtgc tgtttcagca agtgctgcgt aacccgctgg cggaaccgac
     7081 gacgttgggc gttgctacag gcgcgcaact ggggattacc gtcaccacgc tctgggcgat
     7141 ccccggtgct atggcgagcc agtttgccgc gctggtgggc gcttgtgtcg ttggcttaat
     7201 cgtctttggc gtcgcgtggg ggaaacggct ttcgccggtt acgcttattc tcgcggggct
     7261 ggtggtgagc ctttattgcg gggcaattaa tcagctactg gttatcttcc atcacgacca
     7321 gctgcaaagc atgttcctgt ggagcaccgg aacgctgacg caaaccgact ggggcggcgt
     7381 tgagcgttta tggccgcagc tgctgggcgg cgtgatgctg acgttactgc tacttcgtcc
     7441 gttaaccctg atggggcttg atgatggcgt ggcgcgcaat ctcgggctgg ccttatcgct
     7501 cgcgcgtctg gctgcgctgt cgctggcgat agtcatcagt gcgctgctgg tgaatgcggt
     7561 ggggattatc ggctttatcg ggttgttcgc gccactgctg gcaaaaatgc tgggggcgcg
     7621 gcgtttgttg ccacggttga tgctggcgtc gctgattggt gcgttaattc tgtggctttc
     7681 tgatcaaatc atcctctggc tgactcgcgt gtggatggaa gtgtccaccg gttcggtcac
     7741 tgcgttgatc ggtgcgccgc tgctactgtg gctgctgccg cgtttacgga gcattagcgc
     7801 gccggatatg aaggtcaacg attgtgtcgc gactgaacgc caacatgtgc tggcgtttgt
     7861 cctcgcgggc ggcgtgctgc tgttgatggc tgtggtagtg gcgctgtcgt ttggtcgtga
     7921 tgcgcacggc tggacgtggg cgagcggggc gttgcttgag gatttgatgc cctggcgctg
     7981 gccgcgaatt atggcggcgc tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat
     8041 tcagcggctg accggaaacc cgatggcaag cccggaagtg ctggggatta gctctggcgc
     8101 agcgtttggc gtggtgttga tgctgtttct ggtgccgggt aatgcctttg gctggctgtt
     8161 acctgcaggc agtctcgggg cggcggtgac gctgttgatc attatgatcg ccgccggacg
     8221 cggtggattt tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcgttcac
     8281 catgcttttg atgatgttgc aggcaagtgg tgacccgcga atggcgcaag tgctgacctg
     8341 gatttccggt tcgacctaca acgcgaccga tgcgcaggtc tggcgcaccg gaattgtgat
     8401 ggtgattttg ctggcgatta ccccgctgtg ccgccgctgg ctgaccattt taccgctggg
     8461 tggtgatacc gcccgagccg taggaatggc gctaacgccg acgcgaattg cgctgctgct
     8521 gttagcggct tgcctgacgg cgaccgcgac gatgactatt ggaccgttga gttttgttgg
     8581 tttaatggca ccgcatattg cgcggatgat gggctttcga cggacgatgc cgcacatcgt
     8641 aatttcggcg ctggtgggtg gtttactgct ggtgttcgct gactggtgtg ggcggatggt
     8701 gctgtttcca ttccagatcc cggcggggct gctgtcaacc tttatcggcg cgccatattt
     8761 tatctatttg ttgagaaagc agagccgtta attgaatgcc tcgcccggtg ggcgaggcaa
     8821 aatcatcaca acttcgcaaa cacccgtcgt gcagcatcga tggtgttatt gatatcttcc
     8881 atgctgtgcg ccacggacat aaagcccgct tcaaacgctg acggtgccag gtaaacacct
     8941 tcgtccagca tcatatggaa gaaacgctta aagcgttcca cgtcacaggc catcacatcc
     9001 tgatagcacg tcacggactc ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg
     9061 ttaacgacca gcggaattcc ggcttcttct gccgcttcca gcagaccttc tgccagacgt
     9121 gttgtcagct catccagcgt ttcgtgaacg cccggctgcg cgacttcatt cagacaggcg
     9181 aaacccgctg ccatcgcaat tgggttaccg gaaagcgtac ccgcctgata gaccggaccc
     9241 gtcggggcca gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca
     9301 ccgccgatga tttttccgag gcaggttaaa tctggcacta cgccgtaata atcctgtgca
     9361 ccagccagtg ccacgcggaa gccagtcatt acttcatcga tgatcagcaa tgcgccaaat
     9421 tcgtcgcaca gcgcgcgcag acctggcagg aactctggca gcggcggaac acagttcata
     9481 ttgcctgcca ccggctcaac gataatacag gcaatctctt gcgggtattg ctcaaacgcg
     9541 gcgcgtacag aagccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc
     9601 ggaacacccg gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc
     9661 aggcagtcag cgtgaccgtg gtaacaacct tcaaatttaa tgattttgtc gcgaccggta
     9721 aaaccacggg ccaggcggat ggcgctcatg gtcgcttcag tgccggagtt caccatgcgc
     9781 accatatcca tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg
     9841 gttggtgcac caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggattgcc
     9901 ggatggttat ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttt
     9961 ccatcaacat cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc
    10021 acgccagtaa aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg
    10081 ctgtaaagat tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc
    10141 tgctattcta tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact
    10201 ggtgcaatcc tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc
    10261 aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag
    10321 tgatcattgg atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg
    10381 acgcagacaa ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt
    10441 tatggcggca gtcgtcggca cgcttgtcgg gctggcagcg gttgcttttg acaaaggtgt
    10501 cgcctggttg cagaaccaac gtatgggggc gctggtacat actgctgata attatccgct
    10561 actgttaacc gtcgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt
    10621 gcgcaaatac gcgcccgaag cagggggatc ggggatcccg gaaattgaag gggcgctgga
    10681 agatcaacgt cctgttcgct ggtggcgtgt attgccggtg aaattctttg gcgggctggg
    10741 gacactcggc ggaggcatgg tgctagggcg cgaaggacta accgtgcaga tcggcggcaa
    10801 tatcggtcgc atggtgcttg atattttccg cctgaaaggt gatgaagctc gccatacgct
    10861 gctggcaacc ggtgctgctg cggggctggc tgcggccttt aacgcgccgc tggcgggtat
    10921 tttgtttatt atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc
    10981 ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc
    11041 gttgattgac gtcggtaaac tttctgatgc gccgcttaat acgctgtggc tttatctgat
    11101 cctcggtatt atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca
    11161 ggatttgctg caccgtgtgc acggcggcaa tattaccaaa tgggtactga tgggtggtgc
    11221 gattggcggt ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt
    11281 taacctgatt cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt
    11341 cgtcgctcgg gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt
    11401 tgccccgatg ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga
    11461 gctgtttccg caatatcacc tggaggcggg gacgtttgct attgccggaa tgggggcatt
    11521 actggcggca tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga
    11581 taactaccag ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca
    11641 atttaccggc gggaaaccgc tatactcggc gattcttgcg cgcacgctgg caaaacagga
    11701 agctgagcaa ctggcgcgaa gcaaggctgc atcagccagc gagaatactt gaacgaaata
    11761 ccagggtatt agataatggc gattattatt gggttagaat ttgcccaatt gccgatgtcg
    11821 tttggagcaa aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc
    11881 caacaaagtt aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta
    11941 tatcaccggt ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa
    12001 cgaaggcgat atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct
    12061 gcaatatctg gtcggcggtt ccgttgacta caccgaagga ctggaaggtt ctcgtttcat
    12121 cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt tcttccttta gcatctaatc
    12181 tgttgttcat gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca
    12241 tccagcgcaa acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc
    12301 gtcacgacca tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc
    12361 gtagcgtgga caataccgcc gataatacag gcagttgcgt agatttctgt acgtaaaatc
    12421 atggggatct cgcgggccag tacatcacga atgatcccgc cgccaacgcc agtaatgacg
    12481 cccatacaaa ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgccaatg
    12541 ccgacaaaca ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagcgt
    12601 cttggctggc gcaccagcac gatggtcagc atgctggtga ccattgcaac gaccagatcg
    12661 gtgggatctt tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgttccg
    12721 ccgcctactg cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt
    12781 ccggctaaca aaacgccgga gatggcaaat accgctgtgc cgactatatc cagccaatag
    12841 acgagcattg ttcaatcccc actgagcacc tgaaaaggtc aggcactaat ctacctgtga
    12901 aagcgcatta cagagctgtt gtgcagcgag gataatacgt gggcttgcac gttcaaacca
    12961 gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt
    13021 aggaatttgg tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg ctaacacctg
    13081 ttcgcggcta acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca
    13141 aacttcgagt acctggttct gaatcgactc ttttccactg gtaaatggcg gattaatgcc
    13201 gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta
    13261 ctgatccagc agggattgcg cggcttgttc ggccttgtct ggttgcggac tccagggggc
    13321 cagttgacgt aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt
    13381 tattcccagc gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat
    13441 caccagatcg ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac
    13501 ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt
    13561 gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc
    13621 ggcgttgagc cacagtggcg caagaaaaga cagggcgacc agcgccctga acagtgactt
    13681 agccatgtgc aagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa
    13741 cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc
    13801 gtacgacaac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca
    13861 tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt
    13921 tgatgaaagc gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa
    13981 tgcaggcctc agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg
    14041 gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgtgcttcgt
    14101 ccgagacaac gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt
    14161 taataatcac atctggcttg cagtgttcca acagcagagt ggcacccagc gccgcagcga
    14221 ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga
    14281 tttcgcaacc gccgagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa
    14341 cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga
    14401 tttgaaggca tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac
    14461 atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag
    14521 ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc
    14581 aactctccgg caattcgtcg tctgcaacaa aagacgcagg tttttccatt ggagcgcaat
    14641 gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg gcgctacatc
    14701 gccaaagaaa ttttaagccg tctgaaagag cttaaattgc tggaagcata cggcctggat
    14761 gaactgaccg gaccttttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc
    14821 aatccgccgt ttggtcattt tggcgaagca gcgataaatg actggtttcg ccaacgtttg
    14881 catccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggcacta
    14941 cgtttacggg acggggaaga accgcttaac gagctgcggc gcaagattcg tcaggactta
    15001 tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat
    15061 ctcacctggg cacaggttgg cggtatttta aaatataccc gtccggcgtg gtggcgtggc
    15121 gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa
    15181 gcctatattg cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta
    15241 acgtggatta tggaagctgc cgacgacatc tcctattgtg tggcagacct tgaagatgcg
    15301 gtagagaaaa gaatatttac cgttgagcag ctttatcatc atttgcacga agcgtggggc
    15361 cagcatgaga aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc
    15421 tcaaatagtt taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc
    15481 ctaaataaac tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc
    15541 gccggaacgt ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttcttaag
    15601 ctatataaaa atgtcgctgt aaaacatgtg tttagccatc cggatgtcga gcagcttgaa
    15661 ttgcagggct atcgggtcat tagcggatta ttagagattt atcgtccttt attaagcctg
    15721 tcgttatcag actttactga actggtagaa aaagaacggg tgaaacgttt ccctattgaa
    15781 tcgcgcttat tccacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt
    15841 aaattaccgt cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg
    15901 ttgcaggatt atatcagcgg tatgaccgac ctctatgcgt gggatgaata ccgacgtctg
    15961 atggccgtag aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc
    16021 agaaacttta gttcggaact tcgcgctata aaacgaatct gaagaacaca gcaattttgc
    16081 gttatctgtt aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg
    16141 gctctgagtt taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca
    16201 gcaacgacag cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca
    16261 gtggtcagca ttaacgtaga aggtagcaca accgttaata caccgcgtat gccgcgtaat
    16321 ttccagcagt tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc
    16381 tctccgttct gccagggggg ccagggcggt aatggcggcg gccagcaaca aaaattcatg
    16441 gcgctgggtt ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccat
    16501 gttgttgata acgcgacggt gattaaagtc cagctgagcg atggtcgtaa gttcgacgcg
    16561 aagatggttg gcaaagatcc gcgctctgat atcgcgctga tccaaatcca gaacccgaaa
    16621 aacctgaccg caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta
    16681 gcgattggta acccgtttgg tctgggagag acggtaactt ccgggattgt ctctgcgctg
    16741 gggcgtagcg gcctgaatgc cgaaaactac gaaaacttca tccagaccga tgcagcgatc
    16801 aaccgtggta actccggtgg tgcgctggtt aacctgaacg gcgaactgat cggtatcaac
    16861 accgcgatcc tcgcaccgga cggcggcaac atcggtatcg gttttgctat cccgagcaac
    16921 atggtgaaaa acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg
    16981 ggtattatgg ggactgagct gaattccgaa ctggcgaaag cgatgaaagt tgacgcccag
    17041 cgcggtgctt tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc gggcattaaa
    17101 gcgggtgatg tgatcacctc actgaacggt aagccaatca gcagctttgc cgcactgcgt
    17161 gctcaggtgg gtactatgcc ggtgggcagc aaactgaccc tgggcttgct gcgcgacggt
    17221 aagcaggtca acgtgaatct ggaactgcag cagagcagcc agaatcaggt tgattccagc
    17281 tccatcttca acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg
    17341 gtagtgaaca acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat
    17401 gtgattattg gcgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg taaagtgctc
    17461 gacagcaaac cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg
    17521 ttaatgcagt aatctccctc aaccccttcc tgaatacggg aaggggttct ccttacaatc
    17581 tgtgaacttc gccacaactc catacatctt catcatcctt tgggcatttg cacaatgccg
    17641 tacgttacgt acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca
    17701 tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa
    17761 tatcaacgta atggatgccc gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg
    17821 tgaattgcac gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga
    17881 cgcggtagca cgtcatctgc acggtgtgcg gcaggggatt aatctaccgt tacggctgga
    17941 aggtgaaatt gtcggcgtaa ttggcctgac aggtgaacca gagaacctgc gtaaatatgg
    18001 cgaactggtc tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacctgtt
    18061 ggcgcaggat agccgtttgc gggaagaact ggtgatgaac ctgattcagg ctgaggagaa
    18121 tactcccgca cttactgaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt
    18181 ggtggctatt gttgaggtcg acagcggtca gcttggcgtg gacagcgcaa tggcggagtt
    18241 acaacaactg caaaatgcgc tgactacgcc cgagcgtaat aatctggtgg cgattgtctc
    18301 gctaaccgaa atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggatgcaga
    18361 agatcatcgt aagcgagttg aacaactgat tacccgcatg aaagagtacg gccagttgcg
    18421 ttttcgcgtt tcgctgggca actattttac cggtcctggc agtattgccc gatcctatcg
    18481 tacggcgaaa acgacgatgg tggtgggtaa acagcggatg ccagaaagtc gctgctattt
    18541 ttatcaggat ctgatgttac ctgtgttact cgacagtttg cgtggcgact ggcaggccaa
    18601 tgaactggcg cgaccgctgg cgcggctgaa agcgatggac aataatggct tgctgcgacg
    18661 aacgctggcg gcgtggtttc gccacaatgt gcaaccgctg gctacgtcaa aggcgctgtt
    18721 tattcatcgt aatactctgg agtatcggct taatcgtata tcggaactga ccgggcttga
    18781 tttgggtaat tttgatgaca ggttgctgct gtatgtggcg ttgcagctgg atgaagagcg
    18841 gtaggttatg cgttaaggtc gaggcggcgc tggctcattc ccctcagccc gaagggtaga
    18901 ggggaatgtc cgagttaacc gtcagttcgg cattatttac gcgttagttt ttccagatcg
    18961 gcttcaatct cgctgatctt attggttacg actgactcca ggtgacgtaa gtcgtcgagg
    19021 atcttacgct taagatcaac ttcgctgcgg tcacgctggc agatttgatc aagctcatca
    19081 atgatatacc gtagattcgg gctgatttcc tggacttctt tataaccctg acccacacca
    19141 tcagcgacga ccgttttacg ctgacgcgga tatttaaact taacgctctt ggcgaaaaac
    19201 tcgcctttgt ctttctggaa atagattttc agaatatcgt tgttggcttc ctgccggagg
    19261 ctgtaacgat caatttcttc aggattggta atacccagac ttttcagatt gtcgtacata
    19321 gcgttacctc aaaatgagtc agtaaattgt gcttatttta gcatttggcc tggcccgccc
    19381 ggctcgactg tgatcgcaga aagccttttt cttccagact tctatcatgg cgtaaaaatc
    19441 aaaaaattac ctgctttatt gtggtgatat tccatgggat ggattaacac atttatcttc
    19501 gttttgcgaa ttacatgaca gtccgaaaca agatgaaaaa gtatttaaaa aaggggggaa
    19561 gaagaatgag taggacgaat tattctgagc cagaaagcat acgggcagca tcatgctgcc
    19621 cgtatatgta ctcttaatcg atggtacgca gcagttcgtt aatgccgact ttgccgcgag
    19681 ttttcgcgtc aactttctta acgataaccg cacagtagag gctgtatttg ccatctttcg
    19741 acggcagatt acctgaaaca accacagacc ccgccggaac gcgaccgtag tggatttcgc
    19801 cggtttcacg gtcgtaaata cgggtgctct gaccaatgta tacgcccata gagatgacgg
    19861 aaccttcttc gacaatcacc ccttcaacca cttcggagcg tgcgccaatg aagcagttat
    19921 cttcaataat ggtcgggtta gcctgcagcg gctccagcac gccgccgatg cccacgccac
    19981 cggaaaggtg gacgttttta ccaatctgcg cacaagaacc gacggtcgcc caggtatcaa
    20041 ccatcgtgcc ttcatcaaca tatgcgccga tgttgacgta agacggcatc agcacggtgt
    20101 tacgggcaat aaatgcaccc tgacgtaccg ccgctggtgg cacaacgcgg aagccttctt
    20161 tctggaaacg tgcttcgtcg tagtcggcga atttcatcgg cactttgtcg aagtagcggc
    20221 tttctgcccc ttcgatcacc tgattatcat taatacggaa agagagcagc accgcttttt
    20281 tcaaccactg atgcgtcacc cactgaccgt caattttttc cgctacacgc agtgcaccgg
    20341 aatccagcag ggcgatcacc tgatttaccg cttcgcgggt aacggtgtct gcgttggctg
    20401 gcgtgatctc ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac tgctgcattg
    20461 ttaaactctt ttcatatcag taaacacatc accctttatc gtttggattg agggcctctg
    20521 tcaaccgctg atgcacttcc tgctgcaact cgttattaag tgcacgccgg tcagcggtgg
    20581 caattatgaa taaatcttct actcgctcgc caatggttgt aattcgggca ccatgaagcg
    20641 aaattcccag atcggcaaaa attttcccga ctctcgccag cagtcctggc tggtcgagcg
    20701 cgataagctc aaggaacgat ttgcggtcgg tatgggtcgg caaaaacgtt acttcggttt
    20761 caacagtaaa atggcgtaat ttggcgggtt ggcgacgggg ctgcggtggc tgccagctac
    20821 tttgcgtcag tacttgctcc agaccaaacc gaataacctc atgacgatct gcggacagcg
    20881 ggctgccatc gggttccagc acgataaagg tatccatcgc cataccgtcg cgagtggtga
    20941 aaatttgtgc gtcgtgaaca cttaaattgc ggcggtctaa ttcggcacag acggcggcaa
    21001 acagataggg gcggtccggg ctccagataa aaatctcggt gcctccacgc gtagcctgcg
    21061 ggctaagcaa taccagcggt ttgcttaaat catgctgtaa taaatggcgg gcatgccagg
    21121 ccagttgatt tgggctatgg cggacaaaat agttagcacg acagcgtgac caaatttggt
    21181 gcagcgcctc ttcgtcgatg ttatccatgc gcagtagtgc cagtgcctgg agttggtgat
    21241 ggcgaacccg ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg
    21301 tggcaaaata gagttcgcgc aacagacttt gcttccagct attccacagc gtttcgttgg
    21361 tggcgcaaat gtcagccact gtcaggcata ccagatagcg cagacgattt tccgtttgaa
    21421 cttcttcggc aaactgcttg atgacttcag ggtcctgaat atcgcggcgt tgtgctgtca
    21481 ccgacatcag caagtgctgg cgaaccagcc aggcaaccag ctgtgtttcg cgtgagttca
    21541 gcccgtggag ttctgcaaaa tgcactacat cctgagcacc gagaatggag tggtcgccgc
    21601 cgcgtccttt ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca gttgacggca
    21661 ggcgcggcca gacgtccaca cacaacggat ggcgctggcg cgtttcttca ctggcaaaac
    21721 tctccagttt cagcatcacg cggatagtat gttcatccac cgtgtaggcg tggaacagat
    21781 caaactgcat ctgcccgacg atatgcgacc attgcggcat atacgcgccg agcacgctat
    21841 ggcgatgcat tggcaatagc ccgcgccgca ccgctccggg gtgacgcaga atgctcaaaa
    21901 acagttttcg tgcttccgga atattacaca gcggttgttg cagatggcga cgggcatggc
    21961 gtaactggcg cagcgtggtg gagtaaatgc cggtgatcgc actgttgcgc accatggtgt
    22021 agaacatacg caagatggct tccggctggc gcataaatag tgtttcatca cgcaggtcga
    22081 ttagcgtacc gcgtagctga aactcatcgt cgagtggacg tggtttttcg tcggcgggaa
    22141 gggcgaggat ggcttcatcg aacagttgca gcagcatctg gttgagttca ctgacgcggc
    22201 gtgtaacgcg gaagtaatcc ttcatcatcc gctcgaccgg ttcgttgcct tcaccactgt
    22261 aattcagacg ctgagcgacg ctaagttggc gatcgaataa cagacgatta tcgtaacggc
    22321 tgacgaccag atgcagggca aagcgaatac gccacagtat atgcagacat tcgtttaatt
    22381 ccgcccgctc cgctgaggtt aagaagccaa acccgaccat ttcatccagc gatgttgcgc
    22441 caaaatgacg gcgggccacc cattgcagag tgtgaatatc gcgcaagccg ccagggctac
    22501 ttttgatgtc tggttcaagg ttgtagctgg tgccatggta acgctgatgg cgctggttct
    22561 gttcttcaac tttcgccgcg tagaacttgt cggaaggcca gaatccttcg ctaaaaatat
    22621 gtttttgcag ttcgaggaag agcgcaacat cgccaattaa taagcgggat tcgattaaat
    22681 tggtggcgac ggttaaatcc gataaccctt ccagcatgca ctcttcaagc gtgcgcacac
    22741 tatgaccgac ttccagcttt acatcccaga gcagcgttaa cagctcgccc actttttgcg
    22801 cctgatcgtc cgggagcttt ttacggctta aaatcagtaa atcgatgtca gaaagtggat
    22861 gcagctcgcc acgaccgtag ccaccgacgg cgaccaatgc caggtcggca atctggctga
    22921 atcccgcttc aatccataat cgttgcagga gctggtcgat aaactcggtg cgcgcctcaa
    22981 tcaactgttc tgcagagatc ccattgtcaa aggcatcacc cagccaacgc tggaaagtat
    23041 cgatatgggc ttttatccca ccgacggtta attcatcacg gggccagacg catggatttt
    23101 gcggttgacc gggcagggtg gggagagcgg tgtttgcgta ctgttctgga agggtattca
    23161 ttgtgcgcca cccataagat taaattatcg cattaaaaaa gccggcattc gccggcttca
    23221 tcttattcgt cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag cgtcaaaatt
    23281 tcgcagccgt tatcagtcac cacaatagta tgctcatatt gtgcagacaa gctgcgatct
    23341 ttggttttta ccgtccagcc atctttcatg gtgcggatct cttttttacc cgcgttgacc
    23401 attggctcga tggtgaacgt catcccaggt ttcagtacga cgttggtttc acgggagtca
    23461 tagtgcagca cctgcggttc ttcatggaag ccgcgaccaa taccgtgtcc gcaatattca
    23521 cgaacgacgg agaagccttc tgcttcgaca aatttctgaa tcgccgcacc gatttcgcgc
    23581 agattaatgc ctggtttgac catgcgcagc gccaggtaca ggctttcttg cgtgatgcgg
    23641 cacagacgtt cgcccatgat ggtcggctta ccgacgataa acattttcga ggtatcgccg
    23701 tggaaaccat ctttgattac ggtgacatca atgttaacga tatcgccatc tttcagcagc
    23761 ttcgcatcgt ccgggatacc gtggcacacc acttcattaa tagagatgca aacggatttc
    23821 ggatagccgt gatagccgag gcaggcagaa accgcgtgtt gttcattaac aatgtaatca
    23881 ttacagatgc gatccagctc gccggtgctg acgcccggtt taacatacgg ttcgatcatc
    23941 tccagcactt cggcagccag tcggccagcg acgcgcattt tttcgatatc ttctggggtc
    24001 ttgattgaga tagccattaa ttctgtccat cagcgtcggt gataccgaca atatatatgt
    24061 aagtgccgtc aatggtatca cacccgggca aattgagaat cattctgaat ttcgccaaac
    24121 gtgccactga aggttttcta taatagaaaa ttcgacatct gatgctgtac acagcgccaa
    24181 caattattgg tgtccatgac gtatttgtgg tataaagcgc gccggacttc cgatccattt
    24241 cgtatacaca gactggacgg aagcgacaat ctcactttgt gtaacaacac acacgtatcg
    24301 gcacatattc cggggtgccc tttggggtcg gtaatatggg atacgtggag gcataacccc
    24361 aacttttata tagaggtttt aatcatggca actgtttcca tgcgcgacat gctcaaggct
    24421 ggtgttcact tcggtcacca gacccgttac tggaacccga aaatgaagcc gttcatcttc
    24481 ggtgcgcgta acaaagttca catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa
    24541 gctctggctg aactgaacaa gattgcttct cgcaaaggta aaatcctttt cgttggtact
    24601 aaacgcgctg caagcgaagc ggtgaaagac gctgctctga gctgcgacca gttcttcgtg
    24661 aaccatcgct ggctgggcgg tatgctgact aactggaaaa ccgttcgtca gtccatcaaa
    24721 cgtctgaaag acctggaaac tcagtctcag gacggtactt tcgaaaagct gaccaagaaa
    24781 gaagcgctga tgcgcactcg tgagctggag aaactggaaa acagcctggg cggtatcaaa
    24841 gacatgggcg gtctgccgga cgctctgttt gtaatcgatg ctgaccacga acacattgct
    24901 atcaaagaag caaacaacct gggtattccg gtatttgcta tcgttgatac caactctgat
    24961 ccggacggtg ttgacttcgt tatcccgggt aacgacgacg caatccgtgc tgtgaccctg
    25021 tacctgggcg ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag
    25081 gcggaagaaa gcttcgtaga agctgagtaa taaggcttga taactccccc aaaatagttc
    25141 gagttgcaga aaggcggcaa gctcgagaat tcccgggagc ttacatcagt aagtgaccgg
    25201 gatgagcgag cgaagacaac gcatctgcgg cgcgaaatat gaagggggag agcccttata
    25261 gaccaggtag tacacgtttg gttagggggc ctgcatatgg cccccttttt cacttttata
    25321 tctgtgcggt ttaatgccgg gcagatcaca tctccgagga ttttagaatg gctgaaatta
    25381 ccgcatccct ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa
    25441 aagcactgac tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg
    25501 gtgctattaa agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca
    25561 aaatcgacgg caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa
    25621 aagacgctgg tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa
    25681 tcactgacgt tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga
    25741 aaattggtga aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt
    25801 cttatcagca cggtgcgcgt atcggcgttc tggttgctgc taaaggcgct gacgaagagc
    25861 tggttaaaca catcgctatg cacgttgctg caagcaagcc agaattcatc aaaccagaag
    25921 acgtatccgc tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt
    25981 ctggtaagcc gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg
    26041 gcgaagtttc tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc
    26101 tgctgaaaga gcataacgct gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca
    26161 tcgagaaagt tgagactgac tttgcggcag aagttgctgc gatgtccaag cagtcttaat
    26221 tatcaaaaag gagccgcctg agggcggctt ctttttgtgc ccatcttgta aattcagcta
    26281 acccttgtgg ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa
    26341 tcgttgacag tctcaggaaa gaaacatggc taccaatgca aaacccgtct ataaacgcat
    26401 tctgcttaag ttgagtggcg aagctctgca gggcactgaa ggcttcggta ttgatgcaag
    26461 catactggat cgtatggctc aggaaatcaa agaactggtt gaactgggta ttcaggttgg
    26521 tgtggtgatt ggtgggggta acctgttccg tggcgctggt ctggcgaaag cgggtatgaa
    26581 ccgcgttgtg ggcgaccaca tggggatgct ggcgaccgta atgaacggcc tggcaatgcg
    26641 tgatgcactg caccgcgcct atgtgaacgc tcgtctgatg tccgctattc cattgaatgg
    26701 cgtgtgcgac agctacagct gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt
    26761 gatcctctcc gccggtacag gtaacccgtt ctttaccacc gactcagcag cttgcctgcg
    26821 tggtatcgaa attgaagccg atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac
    26881 tgctgatccg gcgaaagatc caactgcaac catgtacgag caactgactt acagcgaagt
    26941 gctggaaaaa gagctgaaag tcatggacct ggcggccttc acgctggctc gtgaccataa
    27001 attaccgatt cgtgttttca acatgaacaa accgggtgcg ctgcgccgtg tggtaatggg
    27061 tgaaaaagaa gggactttaa tcacggaata attcccgtga tggataaata agggtaagat
    27121 tccgcataag tatcgcgggg cgtaagtctg gttataaggc gttattgttg caggcagttt
    27181 ggtcacggcc agcgcgcagc aaccggtgcg tacacaagta cgtgaggatg gcgagcacta
    27241 cccggggcca aaatggcaaa taaaatagcc taataatcca gacgattacc cgtaatatgt
    27301 ttaatcaggg ctatacttag cacacttcca ctgtgtgtga ctgtctggtc tgactgagac
    27361 aagttttcaa ggattcgtaa cgtgattagc gatatcagaa aagatgctga agtacgcatg
    27421 gacaaatgcg tagaagcgtt caaaacccaa atcagcaaaa tacgcacggg tcgtgcttct
    27481 cccagcctgc tggatggcat tgtcgtggaa tattacggca cgccgacgcc gctgcgtcag
    27541 ctggcaagcg taacggtaga agattcccgt ac
//
LOCUS       DAFATA010000056        27086 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFATA010000056 DAFATA010000000
VERSION     DAFATA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.057"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..671)
                     /locus_tag="LGJ20_002826"
     CDS             complement(<1..671)
                     /locus_tag="LGJ20_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBK2386861.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRI"
     gene            complement(801..1532)
                     /gene="pgeF"
                     /locus_tag="LGJ20_002827"
     CDS             complement(801..1532)
                     /gene="pgeF"
                     /locus_tag="LGJ20_002827"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBK2386862.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRVGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(1529..2509)
                     /gene="rluD"
                     /locus_tag="LGJ20_002828"
     CDS             complement(1529..2509)
                     /gene="rluD"
                     /locus_tag="LGJ20_002828"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBK2386863.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            2644..3381
                     /gene="bamD"
                     /locus_tag="LGJ20_002829"
     CDS             2644..3381
                     /gene="bamD"
                     /locus_tag="LGJ20_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBK2386864.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            3652..3993
                     /gene="raiA"
                     /locus_tag="LGJ20_002830"
     CDS             3652..3993
                     /gene="raiA"
                     /locus_tag="LGJ20_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK2386865.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    4096..4221
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4097..4144
                     /gene="pheL"
                     /locus_tag="LGJ20_002831"
     CDS             4097..4144
                     /gene="pheL"
                     /locus_tag="LGJ20_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBK2386866.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            4243..5403
                     /gene="pheA"
                     /locus_tag="LGJ20_002832"
     CDS             4243..5403
                     /gene="pheA"
                     /locus_tag="LGJ20_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBK2386867.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(5446..6567)
                     /gene="tyrA"
                     /locus_tag="LGJ20_002833"
     CDS             complement(5446..6567)
                     /gene="tyrA"
                     /locus_tag="LGJ20_002833"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBK2386868.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(6578..7648)
                     /gene="aroF"
                     /locus_tag="LGJ20_002834"
     CDS             complement(6578..7648)
                     /gene="aroF"
                     /locus_tag="LGJ20_002834"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBK2386869.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            7858..8223
                     /locus_tag="LGJ20_002835"
     CDS             7858..8223
                     /locus_tag="LGJ20_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBK2386870.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            8373..8891
                     /locus_tag="LGJ20_002836"
     CDS             8373..8891
                     /locus_tag="LGJ20_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBK2386871.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            8881..10107
                     /gene="dgcN"
                     /locus_tag="LGJ20_002837"
     CDS             8881..10107
                     /gene="dgcN"
                     /locus_tag="LGJ20_002837"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBK2386872.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            10123..10605
                     /locus_tag="LGJ20_002838"
     CDS             10123..10605
                     /locus_tag="LGJ20_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBK2386873.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10682..11029)
                     /gene="rplS"
                     /locus_tag="LGJ20_002839"
     CDS             complement(10682..11029)
                     /gene="rplS"
                     /locus_tag="LGJ20_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK2386874.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(11071..11838)
                     /gene="trmD"
                     /locus_tag="LGJ20_002840"
     CDS             complement(11071..11838)
                     /gene="trmD"
                     /locus_tag="LGJ20_002840"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK2386875.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(11869..12417)
                     /gene="rimM"
                     /locus_tag="LGJ20_002841"
     CDS             complement(11869..12417)
                     /gene="rimM"
                     /locus_tag="LGJ20_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK2386876.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(12436..12684)
                     /gene="rpsP"
                     /locus_tag="LGJ20_002842"
     CDS             complement(12436..12684)
                     /gene="rpsP"
                     /locus_tag="LGJ20_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK2386877.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(12821..14182)
                     /gene="ffh"
                     /locus_tag="LGJ20_002843"
     CDS             complement(12821..14182)
                     /gene="ffh"
                     /locus_tag="LGJ20_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK2386878.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            14349..15140
                     /locus_tag="LGJ20_002844"
     CDS             14349..15140
                     /locus_tag="LGJ20_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBK2386879.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKTL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            15161..16447
                     /locus_tag="LGJ20_002845"
     CDS             15161..16447
                     /locus_tag="LGJ20_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK2386880.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(16502..17095)
                     /gene="grpE"
                     /locus_tag="LGJ20_002846"
     CDS             complement(16502..17095)
                     /gene="grpE"
                     /locus_tag="LGJ20_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK2386881.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            17218..18096
                     /gene="nadK"
                     /locus_tag="LGJ20_002847"
     CDS             17218..18096
                     /gene="nadK"
                     /locus_tag="LGJ20_002847"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBK2386882.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            18182..19843
                     /gene="recN"
                     /locus_tag="LGJ20_002848"
     CDS             18182..19843
                     /gene="recN"
                     /locus_tag="LGJ20_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK2386883.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            19992..20333
                     /gene="bamE"
                     /locus_tag="LGJ20_002849"
     CDS             19992..20333
                     /gene="bamE"
                     /locus_tag="LGJ20_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBK2386884.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(20395..20685)
                     /locus_tag="LGJ20_002850"
     CDS             complement(20395..20685)
                     /locus_tag="LGJ20_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBK2386885.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(20675..21112)
                     /gene="ratA"
                     /locus_tag="LGJ20_002851"
     CDS             complement(20675..21112)
                     /gene="ratA"
                     /locus_tag="LGJ20_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBK2386886.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            21283..21765
                     /gene="smpB"
                     /locus_tag="LGJ20_002852"
     CDS             21283..21765
                     /gene="smpB"
                     /locus_tag="LGJ20_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK2386887.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            21980..22342
                     /gene="ssrA"
                     /locus_tag="LGJ20_002853"
     tmRNA           21980..22342
                     /gene="ssrA"
                     /locus_tag="LGJ20_002853"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            22571..23785
                     /locus_tag="LGJ20_002854"
     CDS             22571..23785
                     /locus_tag="LGJ20_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HBK2386888.1"
                     /translation="MYMSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPS
                     NIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQARAIYYNNRYFGKTRNEKRITRW
                     GRGSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTL
                     ISGPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQL
                     IDRRRVEYDIFLLVEELHVLDIIKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEK
                     LFIEHGLRHFSTQAVTEGNKKPDFLFPSAEAYHNAEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAF
                     ITELIELYTELS"
     gene            complement(23820..25223)
                     /locus_tag="LGJ20_002855"
     CDS             complement(23820..25223)
                     /locus_tag="LGJ20_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBK2386889.1"
                     /translation="MLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAELLRKVGKNEW
                     SRETINRWINGKCLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLFAGIGGIRKGF
                     EAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFNLDIREVTLSDKTDVLETDAYAYI
                     DEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIIDGKHFLPQHRE
                     RIVLVGFRRDLNIHKGFTLRDISRFYPEHRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGEADFMNES
                     NQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVPVFEAVARLLE
                     PYILKAVSADAGKTGQP"
     gene            25655..26428
                     /locus_tag="LGJ20_002856"
     CDS             25655..26428
                     /locus_tag="LGJ20_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386890.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEVILATVRGIVRGVFIADKWLKSTRENFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(26486..27040)
                     /gene="pinE"
                     /locus_tag="LGJ20_002857"
     CDS             complement(26486..27040)
                     /gene="pinE"
                     /locus_tag="LGJ20_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HBK2386891.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGISTLYKKFPVGDK"
BASE COUNT         7096 a         6986 c         6241 g         6763 t
ORIGIN      
        1 ataatacgct gcgccagacc ttcaacgatg gcagttttac cgacgccggg ttcaccaatc
       61 agtaccgggt tatttttagt acgacgttgc agcacctgaa tggtacggcg aatttcttca
      121 tcacgaccaa tcaccggatc gagtttgccc tgttcggctc gttcggtaag gtcgatggta
      181 tattttttca aagcctgacg ttggtcttca gcaccttgat cgttcacgct ttcacctcca
      241 cgcatttgtt caatcgcttg agtaatgttg gcggtggtcg cccctgctgc tttcaggatg
      301 tcggccagcg tgccgcgaga ctcaagtgcc gccagaacga acagttctga cgagataaag
      361 ttatcaccac gtttttgcgc cagcttgtcg caaagattaa gaacgcgcac cagatcctgt
      421 gatggctgga catcaccacc agtaccttca acctgcggta aacgatttaa tgcctgattg
      481 atatctgtgc gcaactggcc agcattgatg ccagcggatg ttaataaagg actaaccgaa
      541 cccccttcct gattcagcag ggcgctcatt aaatgaagtg gttcgataaa ttggttgtcg
      601 tgcccgagtg caagtgattg ggcatcggca agagcaagct ggaatttatt agtaagacga
      661 tccagacgca taactcctcc cataacggat caaagttgct actggagatt aaatgaggtc
      721 atccctcaat tattcaaggt tattgaccag attaatgtga aaagaaaatc acgcgtaccg
      781 gatcgtcttg attctttagg ttatatcagc caaatgaaac ttgccatacg accggtggtc
      841 ttgtcgcgac gataagagaa gaaagtctca ttttccgtat atgtacaacg gtcgccgccg
      901 aaaatttgct caacacccac gttcgccaga cgctgccggg caagctgata aatatccgcc
      961 agatacttat caccatgctg aatgaaagct gcacttgctt tagcgtctac tgccataaac
     1021 gcctcgcgaa cctccacccc cacttcgaac gcgcgtggac caattgccgg ccctaaccag
     1081 gcgagaatat tttccggatt atcagcaaaa caggaaaccg tctcttccag cacgcctgcg
     1141 cacagtccac gccagccagc atgagcggcg gcgacttccg ttcccactcg attgcaaaac
     1201 agcacaggga ggcagtcggc agtcatcact gcgcaaaccg tgccaggcgt attgctataa
     1261 gaggcatcag ctcgttttga ggcataaggt tcgccagtaa gcttaagcac atctttgccg
     1321 tgtacctgct caagccagac cggtttagaa ggcaaattgc ccgcagcaaa aagtcgcttg
     1381 cgattctcct caacgtgatc cgggttatcg ccacaatggg caccgaggtt gagtgagtca
     1441 tacgggggca agctcacgcc gccgatacga gtagagctac aggccgcaac accttttggc
     1501 agcggccact gcgggacaat cagcttactc ataaccagtc cacttcatcc ttatgctctt
     1561 cgaaatcggc gcgcatcacc tcaatcagct ccaccatatc ttgtggaata ggcgcatgcc
     1621 attccatttc gatgccggag atcgggtgat aaagacgcag catggttgca tgaagcgcct
     1681 ggcggtcaaa cttacgcagc atggagataa atgcttccga agcacctttt ggcggacgcg
     1741 gacggccacc ataaaccgga tcgcccacca gcggatgagt gatatgggcc atatgcacgc
     1801 ggatctggtg cgtacgtcca gtttccagac gcaaccgcag acgcgtgtgc acacggaagt
     1861 gttccatgat gcgatagtga gtcaccgccg gtttgcccat cggatgcacc gccatgtgag
     1921 tacgtttggt cgggtggcgg ctgatgggtt catctactgt accgccagcg gtcatatgac
     1981 caatcgccac cgcttcatac tcacgagtaa tttcacgccg ttgcaaagat tcgactaaac
     2041 gcgtctgagc cggaacggtt tttgccacaa ccatcaggcc agtggtgtct ttatccagac
     2101 gatggacgat gcccgcacgt ggtacatcgg caatgggcgg atagtaatga agcaacgcat
     2161 tcagtaccgt gccatccggg ttacccgcgc caggatgtac caccaggtcg cgcggtttat
     2221 taatgacgat gatatcttca tcttcataga cgatatccag cgggatatcc tgcggttcaa
     2281 aacgcgcttc ttcttcaatc tcagcattga tggcaacctg ctcgccaccc aatacttttt
     2341 ctttcggctt atcacaaact ttgccgttta ccagcacgcg ctggtcgagg atccattctt
     2401 ttattcgcga acgtgaataa tccgggaaca tttcggccaa agcctgatct aagcgttgac
     2461 cgagttggtt ttcggacacc gttgcagtga gctgtactcg ttgtgccata tatactgctt
     2521 cttcgtttat cgttggggtt ttacggcttt gccgtttaat atagtgtgct attgtagctg
     2581 gtcttaaccg ggagcaggaa cagagaatct cccgtattac attttgagga aagtcaaaac
     2641 gtcatgacgc gcatgaaata tctggtggca gccgccacac taagcctgtt tttggcgggt
     2701 tgctcggggt caaaggaaga agtacctgat aatccgccaa atgaaattta cgcgactgca
     2761 caacaaaagc tgcaggacgg taactggaga caggcaataa cgcaactgga agcgttagat
     2821 aatcgctatc cgtttggtcc gtattcgcag caggtgcagc tggatctcat ctacgcctac
     2881 tataaaaacg ccgatttgcc gttagcgcag gctgccatcg atcgttttat tcgccttaac
     2941 ccgacccatc cgaatatcga ttatgtcatg tacatgcgtg gcctgaccaa tatggcgctg
     3001 gatgacagtg cgctgcaagg gttctttggc gttgaccgta gcgatcgcga tcctcaacat
     3061 gcacgagctg cgtttagtga cttttccaaa ctggtgcgcg gctatccaaa cagtcagtac
     3121 accaccgatg ccaccaaacg tctggtattc ctgaaagatc gtctggcgaa atatgaatac
     3181 tccgtggccg agtactatac agaacgtggc gcatgggttg ccgtcgttaa ccgcgtagaa
     3241 ggaatgttgc gcgactaccc ggatacccag gctacgcgtg atgcgctgcc gctgatggaa
     3301 aatgcatacc gtcagatgca gatgaatgcg caagctgaaa aagtagcgaa aatcatcgcc
     3361 gcaaacagca gcaatacata acagaaacct gaaatacaaa acggcagccc ttgagctgcc
     3421 gtttttttat tctgttagtt gtgaaactga agcgatttag tcactatcga tctcatcaaa
     3481 tatggctcgc tttgagatat tcctcaagta aaaaaacacc tcttcctgcg atttctcaca
     3541 aaaaagattc gttgacaaaa agtgacaaaa ttatgagatt cccatcacac attttgacat
     3601 caggagcggt atgctgaatt caccaagacg ggaagacaag aggtaaaatt tatgacaatg
     3661 aacattacca gcaaacaaat ggaaattact ccggccatcc gccaacatgt cgcagaccgt
     3721 ctcgccaaac tggaaaaatg gcaaacacat ctgattaatc cacatatcat tctgtccaaa
     3781 gagccacaag ggtttgttgc tgacgccaca atcaatacac ctaacggcgt tctggttgcc
     3841 agtggtaaac atgaagatat gtacactgca attaacgaat tgatcaacaa gctggaacgg
     3901 cagctcaata aactgcagca caaaggcgaa gcacgtcgtg ccgcaacatc ggtgaaagac
     3961 gccaacttcg tcgaagaagt tgaagaagag tagtccttta tattgagtgt atcgccaacg
     4021 cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt acgggtactg tactaaagtc
     4081 acttaaggaa acaaacatga aacacacacc gtttttcttc gcattctttt ttaccttccc
     4141 ctgaatggga ggcgtttcgt cgtgtgaaac agaatgcgaa gacgaacaat aaggcctccc
     4201 aaatcggggg gcctttttta ttgataacaa aaaggcaaca ctatgacatc ggaaaacccg
     4261 ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc attactggca
     4321 gagcggcgcg aattggccgt cgaggtagga aaagccaaac tgctctcgca tcgcccggta
     4381 cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg taaagcgcac
     4441 catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga ttccgtatta
     4501 actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc agcacgcatc
     4561 gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta tgctgcccgt
     4621 cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt taatcaggtg
     4681 gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc cggtgccata
     4741 aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga gatgacgtta
     4801 actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat taatacggtc
     4861 tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta tcctcactgg
     4921 aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca ggcaaaatca
     4981 ccgcatgttg ctgcattggg aagcgaagct ggcggcactt tgtacggttt gcaggtactg
     5041 gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt ggcacgtaaa
     5101 gccattaacg tgtctgacca ggttccggcg aaaacgacgt tgttaatggc gaccgggcaa
     5161 caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct gattatgacc
     5221 cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta tctggatatt
     5281 caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg ggaaattacc
     5341 cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt tgatccaacc
     5401 tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact ggcggttgtc
     5461 attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt aatcgccgaa
     5521 ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct gctccagcaa
     5581 ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat tacgctctga
     5641 cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc cgaccatcgc
     5701 cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct gaacattttc
     5761 ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct gaataaacgc
     5821 catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc agacctgaat
     5881 ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga ccacaacttg
     5941 ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta ccgggccatc
     6001 gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat caaccagaat
     6061 acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt ggattggcac
     6121 actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc agtcatgttg
     6181 ctccagaatc cgcacctgat aacccgagag ggtcagcatc ttctcgaaca ggcgtcccat
     6241 ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg ttttaaatcc
     6301 tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat cctcaatcag
     6361 atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca tagatgcctc
     6421 gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca cttcagcaac
     6481 cagttccaga cgcttagcta ataaattcag cagcgcttta tcgacttcat caatttgatc
     6541 gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga gccgtcagca
     6601 gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc cagctaatgc
     6661 aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctcg gaagactgat
     6721 tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg ccatctttga
     6781 tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta ttggaattac
     6841 cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc tctttttcac
     6901 attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg atgacatgcc
     6961 cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta atgccaacaa
     7021 aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga ctgccgtcgg
     7081 tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg tgagtttgcg
     7141 attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg tattgcgggc
     7201 tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc tcaagcagca
     7261 atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata tggggatcgt
     7321 taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg cgcattacca
     7381 gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca tattccagag
     7441 cagtttccgg atcatgaatg gaacaaggac cacatactac cagcagacga ggatcgcgcc
     7501 cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct tgttgcaggc
     7561 tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg tcggtaatat
     7621 gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc gtttgcgata
     7681 gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt caatccatat
     7741 ttcgtacatt tttatttaca caggcaattt agtcgcgatt tcacccctta cttctgtata
     7801 gataaattta cactcccttt gaaaacaatc cgctatgctt tgaaaaagga gaaagaaatg
     7861 atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca gattgatcct
     7921 tatactcacg cgccaacctt gaccagcacc gactggtatg atgttggtat ggaagatgcg
     7981 atatcgggca gcgccataaa agatgacgat gcatttagcg attcacaggc ggatcgcggt
     8041 ctatacctta aaggatatgc cgaaggacaa aagaaaactt gccagaccga ttttacttat
     8101 gcccgaggac tttccggtaa aagctttcct gccagctgca acaatgttga aaatgccagc
     8161 caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat acgattaaat
     8221 taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt ttatttctca
     8281 cattgatgac ggtcgcatga aaaaatgata ttttaatgag cattctgaaa aaatatcgat
     8341 gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga ctgttcttac
     8401 ttctgattct cctgctgacg ggagcaccaa tactcgctca ggagccttct gacgttgcga
     8461 aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct gcgctatcag
     8521 ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta caagagaatg
     8581 ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag gcgatgattt
     8641 caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg gaattaaccg
     8701 aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag tgcattattg
     8761 gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta aacctggatg
     8821 ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca cggaagaaaa
     8881 atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc attacgcaac
     8941 atcagtatga ccagcatatt tatcactatg atgctgatct ggttgctgct ttccgtgacc
     9001 tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc agcaacaatg
     9061 acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac tgaaacactg
     9121 gcagcgctgg gccaacaagg gcaattttca actgcagaag tacgtgataa gcagcaaaat
     9181 attctggcat cctggcatta cacccgtaag gatccaggcg atactttcag caatttcata
     9241 agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg tgaaaccatt
     9301 ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg gttttcgctc
     9361 gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct cacccgccat
     9421 ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca tgatgtgcgt
     9481 tccaaccgca atttttcccg acgagtttcg gaagaacgta ttgctgagtt tcaccgcttc
     9541 gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg tttacaggcc
     9601 aaaaatgcgc agcttctgcg taccgcacta catgacccat taaccgggct ggctaaccgc
     9661 gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg aaaaacgtcg
     9721 gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg gggtcatgcg
     9781 acgggcgata gagtcttgat tgaaatcgca aaacggttag ctgaatttgg cgggctgcga
     9841 cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga tgtacagtcg
     9901 gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct cccgtttgat
     9961 cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat gaccattgag
    10021 cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca ggccaaacac
    10081 cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa gcacctggta
    10141 gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca gggaaagttt
    10201 actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc cggcgactgg
    10261 tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt gctcccggaa
    10321 agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct aacacatgcc
    10381 cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg cttatcattg
    10441 aaacgggcga atgtcgtggc cgatgcatgg gctatgggtg gacaaattcc acgcagcaat
    10501 ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac cgcccagggc
    10561 cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac gagcaaacag
    10621 acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct taagcgaaat
    10681 cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc aggtagtaca
    10741 gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact accggagagt
    10801 gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca gtgaatgcag
    10861 agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc agacgttttt
    10921 tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg aaggaaggta
    10981 cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata atttaatctc
    11041 ttatcctggg taaactgata tctcgggggc ttacgccatc ccatcatgtt tatgttgctg
    11101 ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag tcagagccag
    11161 gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt tcaaacgcca
    11221 gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca tcccttctaa
    11281 cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg aatcttccgt
    11341 tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt caatcagcgt
    11401 cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt cttcgtcaat
    11461 ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca gaatcaattt
    11521 ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct gtggtgacag
    11581 ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg cgtcccgcaa
    11641 gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt ccacggtacg
    11701 gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca ggccattttt
    11761 aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag gaaacaggct
    11821 aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt aaaaaccagg
    11881 atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa cctgcccatc
    11941 gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg ccttaatgac
    12001 gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat cgtagccttc
    12061 agtggttact acctggcagc ccatcaggtc tttccagtag tagtcaccct cttcaagctg
    12121 aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg ccgcatcacg
    12181 atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc agctttccag
    12241 ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt caaaaatgct
    12301 ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt aagacgaacc
    12361 cattttcccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt tgctcatcat
    12421 gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag caacgcgatc
    12481 agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca ggcgagtgcc
    12541 ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga agcgaccgtt
    12601 gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt tagcgccgtg
    12661 acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa ccggacccca
    12721 tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat ttctggagaa
    12781 aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca ggaaatcccg
    12841 gcggcatcat ccccttcatg cttctcatca tcttcgccat tccgcctttc ttcattttct
    12901 tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttcacgtcc tgcacctgca
    12961 taccgcaacc ggcagcgata cgacgcttac gcgaaccttt gatgatttct ggcttagcgc
    13021 gctctttcat cgtcatcgag ttgatgatgg cttccatacg caccagcact ttatcgtcca
    13081 tctgcgactt aacattatcc gggatctgcc ccatgcccgg cagcttgccc atcaggctag
    13141 ccatgccgcc catatttttc atctggcgca gttgctcaag aaaatcgttg agatcgaagc
    13201 cgtcgccttt tttcagcttg ctggctaatt tctctgcctg cgcgcggtca actttgcttt
    13261 cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac gcgatgcggt
    13321 ccggatggaa cggctccagc gcctcagttt tctcgccaac accgagaaac ttgatcggtt
    13381 tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg tccactttgg
    13441 tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta ttggccgcat
    13501 cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc gacgcatgga
    13561 cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg gtatccacca
    13621 gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaacg atatctaccg
    13681 gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc agcgtctcaa
    13741 gctgtttgat tgccgccggg cgataaacgt cggcagaaac caccagcact ttcttcttgt
    13801 gcttctcgcg caggaactta ccgagtttac caacgctggt tgttttaccg gcaccttgca
    13861 ggcccgccat cagcacgacc gcaggcggtt gcgccgccag gttcagggtc tggttctctt
    13921 cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc cccggcgtca
    13981 ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata aactcacgca
    14041 ctaccggcag agcgacatcc gcctccagca gcgccatgcg cacttcgcgc agcgtatctt
    14101 ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg cgcgacaaac
    14161 gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg gccgcaatcg
    14221 cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt gttatgcaac
    14281 ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct caaactgtcg
    14341 acatcactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc agtcttgcgc
    14401 tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct attatttctg
    14461 cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg cccgacggtg
    14521 atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg atgatctgta
    14581 cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc attgtctatg
    14641 cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac atcacccatc
    14701 tggaagcaac gccagggatg ctggtgcaca ttggcttatc gctcttttcc tatgccacgc
    14761 taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa ctgaagaaca
    14821 agacgctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt aaaatgttcc
    14881 acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc ctgttctaca
    14941 tgcacaactt atttagcatg gaaaatatcg acaaggccgt gctctctatc gtggcgtggt
    15001 ttgtctatat tgtgctgctg tggggacatt atcatgaagg atggcgtgga cgccgcgtcg
    15061 tctggtttaa cgttgcgggt gcagtcatcc tgacactggc ctacttcggc agccgaattg
    15121 tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca tttctactac
    15181 tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt ccgggtccga
    15241 aaccggaatg atgaccctca accgctatcg tctgcgacat atggcgaaac agggtaatcg
    15301 ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa gcctggtgtt
    15361 aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg ttgggatgcg
    15421 tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg tcgtactggt
    15481 ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag tcgcttatcc
    15541 gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct ggttgctgaa
    15601 tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg tggttagcgg
    15661 ctctttgagc aaagaagagt tgcgcactat cgtgcacgaa tcacgctcac aaatttcccg
    15721 tcgcaatcag gatatgctac tgtctgtgct cgatctggaa aagatgaccg ttgatgacat
    15781 catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga aatcgattct
    15841 gcgccaactc tcccactcac ctcacgggcg catcgtgctc taccgtgatt cgctggacga
    15901 cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga aaaaagagtt
    15961 caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgccgg aaggtacgcc
    16021 gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc tggtcgtcaa
    16081 cgagtatggc gacattcagg ggctggtgac ggttgaagat attctggaag agattgtcgg
    16141 cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc aaaacgacgg
    16201 ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct ttaactggca
    16261 tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac tggaagagat
    16321 ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atagatattc tcgacgtaca
    16381 ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg agagtgtggc
    16441 ggagtaacga aaacggccct gcattcgcat gcagggccgt taattattac gaaaacagaa
    16501 attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga ccattcagcg
    16561 tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca gattccacca
    16621 ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg atcacttcaa
    16681 caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg ccttcaacca
    16741 tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc aggctatcaa
    16801 tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct ttttcaatat
    16861 ccagttcagt acgtcgacgc aggttttcca tttcggcttt tacacgcaaa atgccgtcac
    16921 gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt tcatcgcgcg
    16981 gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg tgctgatcca
    17041 tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta ctcatgaatt
    17101 tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga tcagtttcag
    17161 ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct cggaaaaatg
    17221 aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccccac tgcactgaca
    17281 acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat cgttgagcaa
    17341 caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc ggagattggg
    17401 caactagctg atctcgcggt agtcgttggt ggcgacggta atatgctggg cgcggcacgc
    17461 acactcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct gggtttcctg
    17521 actgaccttg accccgataa cgcccagcaa cagttagccg atgtgctgga aggccactac
    17581 atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg ccaaaagcgc
    17641 atcagcaccg cgataaatga agtggtgctt catccaggca aagtggcgca tatgatagag
    17701 ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg actaattatt
    17761 tcgacgccaa caggctccac cgcctattcc ctctctgcag gcggtcctat tctgaccccc
    17821 tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc acgaccactg
    17881 gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa cgacctggaa
    17941 atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt cctgattcgt
    18001 cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt caacacatta
    18061 agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct ctttactgta
    18121 tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca ggaaaacgac
    18181 tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg agattgattt
    18241 tcatagcggc atgaccgtaa taactggcga gaccggcgcg ggtaaatcta ttgcaataga
    18301 tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta ccggcgctgc
    18361 tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggctc tgcgctggct
    18421 ggaagaaaat cagcttgaag acgggcatga atgtttgctt cgtcgagtga tcagcagcga
    18481 tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac tgcgcgaact
    18541 gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca ccaaacctga
    18601 gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctactgc aggaaatgac
    18661 cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc aacagttaag
    18721 tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac ttaacgaatt
    18781 taatccgcag cccggagagt ttgaacaaat cgacgaagag tacaaacgtc tggcgaacag
    18841 cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg gtgaagacgc
    18901 aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga ttggcatgga
    18961 cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga ttgctgaagc
    19021 cagcgatgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc gactatttga
    19081 acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg tcagccctga
    19141 ggcattgcca cagtattacc agtcgctact ggaagaacag cagcaactgg acgatcaggc
    19201 cgactcacaa gaaacgcttg cgctggcggt aacgaaacat catcagcagg cactggaaat
    19261 cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac agctgatcac
    19321 cgacagtatg catgcgctct caatgccgca tgggcagttt acgatcgatg ttaaatttga
    19381 cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa ccaccaaccc
    19441 aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt cccgcatcgc
    19501 actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga tttttgatga
    19561 agtggatgta ggtattagcg gtccaacagc ggcagttgtc ggcaaactgc tgcgtcaact
    19621 cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg gatgtggtca
    19681 tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacgc atatgcaatc
    19741 cctgaataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca gtgaagtcac
    19801 acgtaataca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt tcctgcttca
    19861 cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt aaagtgatga
    19921 aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc ccgaaaagga
    19981 atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta ttgatgttga
    20041 ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac caggggaact
    20101 atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa caagttgcgt
    20161 acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg ttctatgtct
    20221 tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg acctttaaca
    20281 gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac taataataaa
    20341 gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg tctgttattt
    20401 attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa tgagagggcg
    20461 ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg gacggctgta
    20521 aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca agccactggc
    20581 gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc acgcgctgca ggtactgctt
    20641 ctcaggtagc gcataagcca cctcaacggc aattttacct ggcactgtaa acctctttcg
    20701 cacgaaccgt aaaagcctgg accatattag acgccagctc tttaaacacg cgaccaaagg
    20761 cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata cgacacgcct
    20821 cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc ccatccacca
    20881 gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac gttttgctga
    20941 tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac tccagaatcc
    21001 gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc actaactgat
    21061 acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc ataacaattt
    21121 ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa acgctatccc
    21181 ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca ggattgatat
    21241 ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa gaaaaaagca
    21301 cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga atactttatc
    21361 gaagaagagt tcgaagcggg acttgccctg caaggctggg aggttaaatc cctgcgcgca
    21421 ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc atttctgttt
    21481 ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga tcctacccgt
    21541 acccgcaagt tacttctcaa ccaacgcgaa ctggactcat tgtacggtcg cgtcaatcga
    21601 gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg caaagtgaaa
    21661 atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa agaacgcgaa
    21721 tggcaggtgg ataaagcacg tatcatgaaa aacgcccacc gttaaacctg cactccaatt
    21781 attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat cttattggct
    21841 atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat caggctacat
    21901 gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca taaatctggt
    21961 atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg aaacccaagg
    22021 tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc aaacgacgaa
    22081 aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc ctccgctctt
    22141 aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc tgcctggggt
    22201 tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg cagctggcaa
    22261 gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc ggacgcgggt
    22321 tcaactcccg ccagctccac caatcatgat tggacggtgt aaggacaaca ccaacaaaaa
    22381 caggaagtta gcagtctcag caggacaccg accagacggt gaggagacaa aaaaggatac
    22441 gcaaaggagc cgcggctccc gagtgacaca aaagcccgct tatgcgggct ttttgttttt
    22501 cccttaagtt cctagccgct tatctataac tatggaaaaa tgttaaccct gactgtatgc
    22561 taacaaaggg atgtatatgt cggtttttca taactggctg cttgatatcg caagcgggaa
    22621 ttactttatc tacatcaaac gcctttctgc aaacgacact ggcgcaacag gtggccatca
    22681 ggtcggactt tatatcccct caaatatcgt tgaaaagctt tttccatcta tcaatcatac
    22741 tcgcgaactg aacccttcag tcttccttac tgcgcatgta tcatcccatg attgccctga
    22801 tacccaagca cgcgcaattt attacaacaa ccgttatttt ggtaagaccc gaaacgaaaa
    22861 aagaattacg cgctggggga gaggaagtcc attacagaac cctgaaaata caggagctct
    22921 cacaattctt gctttcaggt taaacgaaca gaacactgac tgttccgagg tagatatatg
    22981 ggtctgcgtc aatcccgatg aagaggatat catcgagtct gctattggcg aaatcatacc
    23041 tggaacccta atttccggcc ctgccggaca aattttgggc ggattgtctc ttcagcaaac
    23101 tccagtaaat cataaatatg ttattcctga agactggaag aagcgttttc cttctggaaa
    23161 cgaaattatt caatatgctg ctggccatta tgctaaaaac tccaaggatc cagatgagca
    23221 actgattgac cgtcggcgtg tcgagtatga tatttttcta ctcgtcgagg aattacatgt
    23281 tcttgatatt attaagaaag gattcgattc tgtagatgag tttattgcat tagccaactc
    23341 tgtcagtaat cgacgtaaat caagggcagg taaatcactt gaacttcacc tagagaagct
    23401 ttttatcgag cacggactac ggcatttctc cactcaggca gttactgaag gtaataaaaa
    23461 accagatttc ctgtttcctt cagcagaggc atatcataac gctgaatttc ctgtagaaaa
    23521 cttacgtatg ctggcagtaa agaccacctg caaagatcgc tggcgtcaga tactgaatga
    23581 agcagataaa atccatcagg tacatttatt tacgctgcaa gaaggtgttt cttcagcaca
    23641 ataccgagaa atgaaagatg cgggtgtcag actcgttgta ccatcaactt tacataaaaa
    23701 atacccagaa gcagttagag aagaattaat aacgctcgga gcattcatta ctgagctgat
    23761 agaactttac actgaactat cataggctga ctcccggctt aaaaggccgg gaggtgttct
    23821 caaggctgcc cagtcttacc agcatcagca gaaacagctt tgaggatata gggttccaga
    23881 agtctggcaa cggcttcaaa tactggcacc acaacggagt taccgaactg ccgatatgac
    23941 tgagtatctg aaaccggaat acggaatggt ttccctccag gtttttcaaa ccccataagg
    24001 cgtgcgcact ctcggggagt cagcctgcgt ggtcgacgag cctggttact ctcattcata
    24061 aagtcagcct ctcccgttgc catatcccaa ccacgatcaa taagaatttc tgatccgtct
    24121 ttgtgatatc tggcagaaag tgtgcgtgca atgctttcct tattctcagg attgaccaac
    24181 ccaaagccaa aaccattccc cttggctgca tgctttttgg cgtagttata aaggtactcc
    24241 catagttttg gcgtaagtat atatttactg tcgactacag gctccagcaa ttcaccaaat
    24301 gatgggcggt gttccggata aaaacgactg atatcacgca aggtaaaccc cttgtgaata
    24361 ttcagatctc gtctaaatcc gaccagaaca atgcgttcac gatgctgagg caaaaaatgc
    24421 ttcccatcga taatctttgg atcgtttttt cccatttctg cagcatcggc aacttcgtag
    24481 cccagctcgt caagggtctc cataatgact ttgaaagttt tacccttatc atggctcttc
    24541 agatttttga cattttccag cacaaaaatt gccggttttt ttgctcgtat aatacgtgcc
    24601 acgtcaaaaa aaagtgttcc ttgtgcttca cattcaaaac catgcgcacg accaagtgag
    24661 tttttcttac taacaccagc aaggctaaat ggctggcacg ggaaccccgc aagaagcaca
    24721 tcatgatccg gcacatgctc atcaatatat gcataagcat ccgtttccaa tacatcggtt
    24781 ttatcactca gcgtgacttc ccgaatatcg agattgaatt tatgcacctg ttcatcgtta
    24841 aaccagttgg ccttgtatgt acgcacagca tctttattcc attcactggt aaaaacacac
    24901 tgacctccaa tggcctcaaa acctttccgt atccctccaa ttccagcaaa taagtcaatg
    24961 aagcggaaag catattccgg atggtttgca ggtggttctg gtagcatctt acgcagaaga
    25021 gactcttcta ctgaagtcaa cgattttggt aaacacttgc cattaatcca gcggttaatg
    25081 gtttcacggc tccactcatt ttttccgact tttctcagta attcagccac atacttctga
    25141 tcatagattt ccagcacttt ctcgataagc tttttatcat tttcctgtcg cagcttttct
    25201 tccgcctcgg cttccttcag cagatgctgt gccaacactt caaattcaga cataattcct
    25261 ccaaggggtc taatgggtga aactttatca ctcattcaac ccagaaggaa atgttttatc
    25321 tggatattta aacagtgact acaacgtaat ctagcactgg tgatgctttg ttaggcatag
    25381 agaatcattc tatatacgac taatgacaga aaaacagcag acaagtagtt tgttcataaa
    25441 ttaacgcata caacgtgcct gcggtttttg agaccggtcc gatcatcaaa cgaaacataa
    25501 aattagctca cactatgagg aaaagtatct tttttactat gtaaattcaa aggattagcc
    25561 tcatttaccc gatagttttc tcaacactac tggttgtgag cccttgcaat gttcattaat
    25621 atacgtctca caaataattc atagatattg caaaatggat attactgagt ttccttctgg
    25681 agtaattgaa caccttggct ggtatgtata ccgattgatt gatccgaggg acggaagcac
    25741 cttctatgta gggaaaggca aaggtaaccg cgtatttgcc catatgcgcg gtgaagtggc
    25801 agcgactgat gatgacgagt tactgagcaa caagctaaag caaattagag aaataaggtt
    25861 agcaggactt gaagttatcc atgtcatcca tcgacacgga atgactgatg aaaagacggc
    25921 gtacgaagtt gaagctgcac ttattgatgc ctaccctggg ttaacgaata tcatgaatgg
    25981 tgctggcagc aatgaattcg gcgccgcgca tgtcaaagag ttgatagcaa catatcaacc
    26041 cgaaaccata acatttcatc ataaagcatt aatgatttcc gttaacagaa gtgcaaagga
    26101 ttcagagctt tatgatgcgg ttcgatttag ctggcgcatt aatgtctctc gcgccagcca
    26161 agcagaagtc attcttgcta ctgtaagggg aatcgttcga ggggttttca ttgctgataa
    26221 atggctcaaa tcaacacgtg aaaatttccc tacgatgaaa tactgggacg aggatcctga
    26281 ctttgaggca acacaaagtt ctcgctatgg ttttgaaggc cgagaagccc cacctgaaat
    26341 agcaaatctt tatcttggaa aaaaaatacc agatgaatta agaaaaaaag gagctatgtc
    26401 cccggtccgt tactcaccta atttttgagt ctttaagtga taagcataaa ccgcagcacg
    26461 atcttgcata cgacgtgcta cggtttcatt tatctccgac cggaaacttc ttatacagtg
    26521 tcgatatacc aacatcatag atgatcgcca ccttctggcg aggaacgcct gatgcaatta
    26581 atcgcccggc ctgcgcccat tgttctggtg taagtttggg acgacgtcca ccaattcgtc
    26641 cctgtgcgcg agcagcttcc agtccagctt ttgttcgttc aacaatcagt tctcgttcca
    26701 tttcagccag ggcacccatc acatgaaaga aaaaacgccc catcggtgtg ctggtatcaa
    26761 tagcatccgt caggctgcga aaattaacgc cacgttcgcg caactcctca accagaatga
    26821 ccagatgccg catactacgc cctagccgat ccagcttcca gacaaccaga gtgtcacctg
    26881 ccgataatgt cctgagcagt tttttcagtc ccggcctttc ggactttgta ccgcttatct
    26941 tgtcttcaaa aatcagctcg catcctgcac agttcagcgc attacgttgt agatctgtgt
    27001 tctggtcatt tgttgacaca cgtacatagc caataagcat ggtagatctc cctgacaaaa
    27061 gcaggaatga tgccatttgc tcgtta
//
LOCUS       DAFATA010000057        26604 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFATA010000057 DAFATA010000000
VERSION     DAFATA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.058"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..947)
                     /locus_tag="LGJ20_002858"
     CDS             complement(<1..947)
                     /locus_tag="LGJ20_002858"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBK2386892.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRDN"
     gene            complement(1156..1815)
                     /locus_tag="LGJ20_002859"
     CDS             complement(1156..1815)
                     /locus_tag="LGJ20_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBK2386893.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2142..2498)
                     /locus_tag="LGJ20_002860"
     CDS             complement(2142..2498)
                     /locus_tag="LGJ20_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBK2386894.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2565..2855)
                     /gene="yebG"
                     /locus_tag="LGJ20_002861"
     CDS             complement(2565..2855)
                     /gene="yebG"
                     /locus_tag="LGJ20_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBK2386895.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            2989..4167
                     /gene="purT"
                     /locus_tag="LGJ20_002862"
     CDS             2989..4167
                     /gene="purT"
                     /locus_tag="LGJ20_002862"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBK2386896.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4223..4864)
                     /gene="kdgA"
                     /locus_tag="LGJ20_002863"
                     /gene_synonym="eda"
     CDS             complement(4223..4864)
                     /gene="kdgA"
                     /locus_tag="LGJ20_002863"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK2386897.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4901..6712)
                     /gene="edd"
                     /locus_tag="LGJ20_002864"
     CDS             complement(4901..6712)
                     /gene="edd"
                     /locus_tag="LGJ20_002864"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBK2386898.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6947..8422)
                     /gene="zwf"
                     /locus_tag="LGJ20_002865"
     CDS             complement(6947..8422)
                     /gene="zwf"
                     /locus_tag="LGJ20_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK2386899.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8760..9629
                     /gene="hexR"
                     /locus_tag="LGJ20_002866"
                     /gene_synonym="yebK"
     CDS             8760..9629
                     /gene="hexR"
                     /locus_tag="LGJ20_002866"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBK2386900.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9757..11199
                     /gene="pyk"
                     /locus_tag="LGJ20_002867"
     CDS             9757..11199
                     /gene="pyk"
                     /locus_tag="LGJ20_002867"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK2386901.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11330..12301)
                     /gene="lpxM"
                     /locus_tag="LGJ20_002868"
                     /gene_synonym="msbB"
     CDS             complement(11330..12301)
                     /gene="lpxM"
                     /locus_tag="LGJ20_002868"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBK2386902.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12421..13743)
                     /gene="mepM"
                     /locus_tag="LGJ20_002869"
     CDS             complement(12421..13743)
                     /gene="mepM"
                     /locus_tag="LGJ20_002869"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBK2386903.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(13759..14691)
                     /gene="znuA"
                     /locus_tag="LGJ20_002870"
     CDS             complement(13759..14691)
                     /gene="znuA"
                     /locus_tag="LGJ20_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBK2386904.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            14770..15525
                     /gene="znuC"
                     /locus_tag="LGJ20_002871"
     CDS             14770..15525
                     /gene="znuC"
                     /locus_tag="LGJ20_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBK2386905.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            15522..16307
                     /gene="znuB"
                     /locus_tag="LGJ20_002872"
     CDS             15522..16307
                     /gene="znuB"
                     /locus_tag="LGJ20_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBK2386906.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(16454..17464)
                     /gene="ruvB"
                     /locus_tag="LGJ20_002873"
     CDS             complement(16454..17464)
                     /gene="ruvB"
                     /locus_tag="LGJ20_002873"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK2386907.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(17473..18084)
                     /gene="ruvA"
                     /locus_tag="LGJ20_002874"
     CDS             complement(17473..18084)
                     /gene="ruvA"
                     /locus_tag="LGJ20_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK2386908.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(18223..18288)
                     /gene="yobI"
                     /locus_tag="LGJ20_002875"
     CDS             complement(18223..18288)
                     /gene="yobI"
                     /locus_tag="LGJ20_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBK2386909.1"
                     /translation="MYIFITHFFTEYVILKYLLPI"
     gene            18359..18961
                     /locus_tag="LGJ20_002876"
     CDS             18359..18961
                     /locus_tag="LGJ20_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBK2386910.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(18963..19484)
                     /gene="ruvC"
                     /locus_tag="LGJ20_002877"
     CDS             complement(18963..19484)
                     /gene="ruvC"
                     /locus_tag="LGJ20_002877"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBK2386911.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(19519..20259)
                     /locus_tag="LGJ20_002878"
     CDS             complement(19519..20259)
                     /locus_tag="LGJ20_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK2386912.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(20288..20740)
                     /gene="nudB"
                     /locus_tag="LGJ20_002879"
     CDS             complement(20288..20740)
                     /gene="nudB"
                     /locus_tag="LGJ20_002879"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBK2386913.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(20858..22630)
                     /gene="aspS"
                     /locus_tag="LGJ20_002880"
     CDS             complement(20858..22630)
                     /gene="aspS"
                     /locus_tag="LGJ20_002880"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK2386914.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            22940..23506
                     /locus_tag="LGJ20_002881"
     CDS             22940..23506
                     /locus_tag="LGJ20_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK2386915.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            23503..24321
                     /locus_tag="LGJ20_002882"
     CDS             23503..24321
                     /locus_tag="LGJ20_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK2386916.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            24374..24769
                     /locus_tag="LGJ20_002883"
     CDS             24374..24769
                     /locus_tag="LGJ20_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBK2386917.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            24810..25553
                     /gene="cmoA"
                     /locus_tag="LGJ20_002884"
     CDS             24810..25553
                     /gene="cmoA"
                     /locus_tag="LGJ20_002884"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBK2386918.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            25550..26521
                     /gene="cmoB"
                     /locus_tag="LGJ20_002885"
     CDS             25550..26521
                     /gene="cmoB"
                     /locus_tag="LGJ20_002885"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBK2386919.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
BASE COUNT         6260 a         6915 c         6660 g         6769 t
ORIGIN      
        1 atgttatcgc gtggcggaat taactcttcc cactgttgct catcacgcag gcgggtacgg
       61 tataagccaa agtttttgcc atggcggttg gaacgcagat aaaaccgatg ctggtagtga
      121 tcaaggctgt attcgtgatc tttgcggcgc ggcagaaaaa caaacggctc ggcatcggcc
      181 atttccgcgt ccagcaggcg aacttcactg gtggtggcgc tggccaaatg aatgactaca
      241 tagtgcttcg aggtcgtttt atgcaggctg acgtaatagg tatcgtcttt ttcttcgtag
      301 atcagtttat cttgcgatgc tggcgtaccg atggcgtgac gccagacctg ataaggcagc
      361 agcgtcaccg gatgcttgcg aacatagtag aaagtccagg agtcatttgc ccagacaaag
      421 ctgggttcaa cgttatccag cagttccggg taccagttac cagtttccag attacgaaaa
      481 cgaatgccgt actggcgtcg ggaaagaaaa tcttctgcca gcgccataat ggtgttatcg
      541 ggcgtaatcg ccattccgcc catcgaataa aactcactat gagctgcgcg cttattggca
      601 tcgagcaatg tttcccactc atcccactct tcactgaatg ccgattgacg ctggtagata
      661 gcatattcac agcctggttc ataaatatgc cgatagcggt agccattttt gatgtagggc
      721 gcagaaactt ctcgttgcgg aatgcggtcg atgatttcct ttaagatgcg atcctgcaag
      781 gcttgttgtg aggccatcac ccgatgaccg taactatttt cttgttgcag gtagtccagg
      841 acttctggct gagaacgcgt atcgtcccgc agccagtagt aattatcgat gcgcgtatcg
      901 ccatgaagcg tcatggcgtg gggaatgcgg gcggcttttg gtagcatgtt attgttcttt
      961 ctggttgaaa catcttataa gggtggcaaa actcaccggg gatgcaagcg aaacagggga
     1021 gtcattgctt agatgatgac aggtaatggc gcggatatcg aatgttatgc agacagagaa
     1081 aatcagcctg ttcaaatggc tgtgcgattc tggatagccc gaaatagtca acttcaggct
     1141 atccagagag cggaattatt ccgccaaagt gcgtttttgc tgttcgagat cgcgttcaat
     1201 gccgtcacga acatcctggg ggattttcag cgcgtcaccc agtgcattca ggtaactgcg
     1261 ttccataaaa tggtcaatat caatagccgc gcaactcagg aaatagattt ccagcgcctc
     1321 ttcttcattg cggaccccgg tagccaggcg ttgtggatcc agcggttgtt cgattgcctg
     1381 ctcaatgagt acacgcccct gctcttccac gccggcttca cgcaattgct ggtcgattgc
     1441 cgcacgttct ttggcatcaa tatgaccatc acttttagcg gcaaagacca gcgcaaggat
     1501 caaacgttct gtacgctcat ccagcggcgt actttgcgcg ccaaactgcg gttcgtcctg
     1561 atgcgccgcg cgaattttat ctttgtattt attccacagc accgtacccg ctaccgctcc
     1621 gccgccaacc agtaacgcgt tggtgccata tttcgtaaga agtttacgtg ctgatttatt
     1681 tgcgaccagc agcccagcca gtccgcctaa tgcgcctggc actaacagtt tgcccaatcc
     1741 ctgatccgca gacgaggagg tagaagaact gctttgccca agcagggatt gcagttgatt
     1801 taaccagtta gccatatttg ctcctcaata accattttta gtgcctgtag catagcggat
     1861 gaggatgtca gaaaatgtat atcgagacaa aagatgcgca aataagcggg gcggtaggcc
     1921 gttattcaaa gaaattcgcg ccaggtaagg tatttactgt atatcaggtg ataagggtat
     1981 tttaggtgaa agtagtttgt cgttttgagt ggcattgttt gatgctgatt gcccgatgcg
     2041 acgctgttgc gtcttctcag gcctacgatt gtggtgcgtt ctggggcgga caaggcactc
     2101 gcgccgcatc cgccaacggt caatcgaatt atcagaagcc attaacgccg ctgatattcc
     2161 gccattcccg ctttgcagtc cacgctgacc tggtaatgaa tatcggcact tttaccacgc
     2221 acggttagcg gtactgacca tttatcatct ttaccctgaa tgtcctgcaa actgacccaa
     2281 gccacgggat cggcctgacc gacaattttt tgatcatctg cccagcgcgc cacgcgattt
     2341 tgttgataat cacgttttac gctcgcggca attccggcag catccagacc ttcacacttt
     2401 gggaaagtga ccgacttgct ggtttcatta ttggcagcga aaactgatgc gcaggcagaa
     2461 accaacaaca gccctaaaaa cgcccctctt tttttcatgt ttttctccat agcacaatga
     2521 ttcaggagaa agcatggtac aaattgtcag gagcgcaagt tgcttcaggc cgcgtgtgag
     2581 gcatcttcct cttcggattc agcaccgacg acctgtgggg acggtaattt gccggttttc
     2641 agaatggtgc tcagcacatc tttttgttct gccagccata gcgaaagggc ttcacgttgc
     2701 tcgtcttcca tttgcactgg agaatttgtc agccaggtgt caagcagatc cgccgtatca
     2761 agcattttat cataagcatc ggcttccttt ttgctggtaa acgacatttt ctcttcgccc
     2821 tcacgaatga ctacgtattt aacttcaacc gccatttgca gcctctcata ataactgtga
     2881 ttttatacag tatatttctt ttcggttgag aaatcaacat cagcaataaa gacacacgca
     2941 aacgttttcg tttatactgc gcgcggaatt aatcagggga tattcgttat gacgttatta
     3001 ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat taggctccgg tgaactgggt
     3061 aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg tgattgccgt cgatcgctat
     3121 gccgacgcac cagccatgca tgtcgcgcat cgctcccatg tcattaatat gcttgatggt
     3181 gatgcattac gccgtgtggt tgaactggaa aaaccacatt atatcgtgcc ggagatcgaa
     3241 gctattgcca ccgatatgct gatccaactt gaagaggaag gactgaatgt tgtcccctgc
     3301 gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc gtcgcctggc ggcagaagag
     3361 ctgcagctgc ccacttccac ttatcgtttt gccgatagcg aaagcctttt ccgcgaggcg
     3421 gttgccgcca ttggctatcc ctgcattgta aaaccggtga tgagctcttc cggcaagggg
     3481 cagacgttta ttcgttctgc agagcaactt gctcaggcat gggaatacgc tcagcaaggc
     3541 ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg ttaagtttga cttcgaaatt
     3601 accctgctaa ccgtcagcgc ggtggatggc gtccatttct gtgcaccagt aggtcatcgc
     3661 caggaagatg gcgactaccg tgaatcctgg caaccacagc aaatgagccc gcttgccctt
     3721 gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac tgggcggtta tggtctgttt
     3781 ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca gtgaggtctc ccctcgtcca
     3841 catgataccg ggatggtgac gttaatttct caagatctct cagagtttgc cctgcatgta
     3901 cgtgccttcc tcggacttcc tgttggcggg atccgtcagt atggtcctgc agcttctgcc
     3961 gttattctgc cacaactgac cagtcagaat gtcacgtttg ataatgtgca gaatgccgta
     4021 ggcgcagatt tgcagattcg tttatttggt aagccggaaa ttgatggcag ccgtcgtctg
     4081 ggtgttgcgc tggcgaccgc tgagagtgtt gttgacgcca ttgaacgcgc gaagcacgcc
     4141 gccggacagg taaaagtaca gggttaaacc cggcaaaaaa acgctacaaa aatgcccgat
     4201 cgtagatcgg gcgttttgac ttttacagct tagcgccttc tacagcttca cgcgccagct
     4261 tagtaatgcg gtcgtaatcg cccgcttcca gcgcatctgc cggaaccagc caggaaccac
     4321 cgatgcacag cacgcttttc agcgccaggt agtcacggta gttagccgga gaaataccac
     4381 ccgtcgggca gaaacggacc tgggagaacg gacccgcgat cgcctgcagg gctttcacgc
     4441 cgccgttagc ttcagccggg aagaatttga actctttcaa accgtagtcc atacccagca
     4501 tcagttcgga aacagtgctg atccccggaa ttagaggaat agtcccttcg gtagcagctt
     4561 tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg tgcacccgct tcagtgactt
     4621 ctgccagctg ctgtggattc agcaccgtac cggcacccac aatcgcttca ggcacttctt
     4681 tggcgatagc acggatagcg tcaactgcac actcggtacg cagagtcact tccagaacgc
     4741 gcaccccacc agcaaccaac gcttttgcca tcggcactgc gtgttccagt ttttttacca
     4801 cgataaccgg tacaaccggg ccggtggtca ggattgattc tgcacttgtt ttccagtttt
     4861 tcatcagagt tttctctcgc ctgattacaa atttgtcgtc ttaaaaagtg atacaggttg
     4921 cgccctgttc ggcaccggac agtttttcac gcaaggcgct gaataattca cgtcctgtcc
     4981 ccacgcgtga cgcgctcagg tcaggaatgt gcggttcgcg agcagccagt tccgcttcgt
     5041 ctaccagcag cgtcagttcg cctgtctgtc cattcacacg aatgatgtcc ccgtcgcgca
     5101 cttttgccag cagcccgcca tcgtaggctt ctggtgttac gtggatagct gacggcactt
     5161 tacctgaagc gccggagagt cgtccatcgg taactaacgc aattttgaaa caccggtcca
     5221 ataatacacc aagtggcggc atgagtttat gtaattctgg cattccgttc gcttttggcc
     5281 cctgatgacg gacaacaacg acacaatcgc ggtccagcaa acccgcttca aaggccggca
     5341 taacgtcatg ctggctttca aaaacaaccg ctggcgcttc aatcacctgg ttctcaaccg
     5401 gcacggcaga ggttttcata accgcacggc ccaggttacc gcttaacact tttgtcccac
     5461 catgatgaga gaaaggttgt tcgaaggaag cgatcacatt gctgtcgagt gatttttccg
     5521 ccccttcccg ccagtccagt tcaccattat tcagccatgg ttcaagggta taacgagaca
     5581 gaccaaagcc tgccaccgta ttgacatctt catgcagcag gcctgctttg agcagttcac
     5641 gcaccagaac cggtacgcca cctgccgcct ggaagtggtt aatatcggcc ggaccgttcg
     5701 ggtagagacg tgccatcagc ggtacaacat cagaaaggtc ggagaagtca tcccagttaa
     5761 tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat ggtgtggtta gtggaaccac
     5821 cggtcgccag cagagcaaca ataccgttca ccaccacttt ctcatcgatc atcttaccga
     5881 tcggcatcca ttcattgcca ttaccggtca tgcgtgtaac ctgacgcgca gcggcagcgg
     5941 tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga agagcctggc aactgcatcc
     6001 ccataaattc caccaccatc tggttggtgt tggcagtacc gtagaaagta catgttcccg
     6061 gcgcatggta agacgcggct tctgactcca gtaaggccat gcggtccact ttaccttcgg
     6121 cataaagctg gcgaatacgc actttttctt tatttggcaa accgcttgcc atcggtccag
     6181 acggcacaaa caccgcaggc aaatgaccaa acgacagggc tgccatcgtc agacccggga
     6241 caatcttgtc gcacacaccg aggaacagag caccatcaaa catgttatgg gacagcccga
     6301 ccgccgcaga catcgctatc acttcgcggc ttagcagcga caattccatt ccatcctgcc
     6361 cctgggtgac accatcacac atcgccggaa caccgcccgc aacctgacca accgcattcg
     6421 cttcatgcag ggctttacga atgatttctg gatagtgttc ataaggctgg tgcgcggaga
     6481 gcatgtcgtt ataggaggtg atgatggcga tattgttacg caacatgctt ttcaaagagg
     6541 ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag gttaccgcat gccaactgcg
     6601 aacgatgaac ggtcgaagtt ttcgcttgtt ctatccgggc gagataagca gagcgagtct
     6661 cgcgcgaacg ttcaatgatt cgatttgtta cgcgtaacaa ttgtggattc ataaaggctc
     6721 ctgaaattga gttgtcagag caggatgatt cacaacgcgt ttcattcaga ggatttatga
     6781 ctgaaacgcc tgtaaccgga gctcataggg caaaaacgtt tcagtcagtg taataaaaaa
     6841 agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa aattgttcta caatctgtgc
     6901 aagatcatgt taccggtaaa ataaccataa aggataagcg cagatattac tcaaactcat
     6961 tccaggaacg accatcacgg gtaatcatcg ccaccgaggc aacgggtccc caggttccgg
     7021 cctgatacgg tttcggcgca tcattgtcca tcgcccacgc ctcagtaatg gagtctaccc
     7081 atttccaggc ttcttccact tcgtcgcgac gaacaaacag tgcctgaata ccacgcatgg
     7141 tttccagcag caaacgttca taggcatccg ccagatgcgt ctgattaaag gtttctgaat
     7201 agctcagatc cagcttggtg atttgcaggt tatgtttgtg gtcaaggcca ggaactttat
     7261 tcagtacctg gatatccacg ccttcatcag gttgcagacg gatagtcagt ttattctgcg
     7321 gcagatcctg ccacgattct ttaaacagat tcagttcagg tgttttgaaa tagaccacga
     7381 cttcagaaca tttggtcggc agacgtttac cagtacgcag gtagaatggc acaccggccc
     7441 agcgccagtt atcaatgtcg acgcggatcg ctacgaaggt ttctgtattg ctgctcttgt
     7501 tcgcgccctc ttcttccaga tatcccggca cttttttgcc ctgggcgaag cccgcagtat
     7561 attgcccgcg tacggttttt tcgcgtacgt tggagcggtc gatgcggcgc agagacttca
     7621 gtactttcac tttttcatcg cggatgctgt ctgcgctcag gtcagacggc ggagacatcg
     7681 caatcatgca gagaatttgc agcaggtggt tttggatcat atcgcgcatc tgaccggctt
     7741 tatcaaaata gccccagcgc ccttcgatcc ccacttcttc tgccacggta atctcaacat
     7801 gatcaatggt gcgattgtcc cagttattca caaacaggga gttagcaaaa cgcagcgcca
     7861 acaggttcag caccgtttct ttaccaagat agtggtcgat acggtaaacc tggcactcct
     7921 cgaagtattc gccaacctga tcattgattt cctgcgaggt cgccagcgac gtccccagcg
     7981 gtttctccat aactacgcgt gccggtttag cattcagttt tgcctcacca agccctttgc
     8041 aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt aatggtgata cgattttttt
     8101 gatccagcat cgcgccgaga cggctgaatg cagcagtgtc attgacatcg agattacaaa
     8161 aatccagacg tgcactcagg gtgtcccata aaccttcatc aatagtttct ttcatgaaag
     8221 tttcgagcgc ctcacggaca actttggtat aagccgcttt atcccagtca gcacgcccta
     8281 cgccgataat ccgggtgtcc gggttgagct gaccggcttt ttccagttga tacagggaag
     8341 gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa aatgaccagg tcacaggcct
     8401 gggctgtttg cgttaccgcc atgtcattct ccttaagtta actaacccgg tacttaagcc
     8461 agggtatact tgtaattttc ttacggtgca ctgtactgct tttacgagct tgcgaaaact
     8521 gtaaacgctt atccacccgt gcgattacgg gaaaagcgcg caaagtgcgg caaaactgat
     8581 aaaaaaatca tcgttttcca ttcggtaaaa atctgacact gatcatgtta tgaaaaaaaa
     8641 taacaacttt tttatctgct tttgtcatta acacggcaca caggcgtaat atctgacaaa
     8701 actgcatttc gatttctttc agtgcggaaa tcgtcattac ccgtgagtct ctttacatca
     8761 tgaatatgct ggaaaaaatc cagtctcagc tggaacattt gagcaaatca gagcgcaaag
     8821 ttgccgaggt cattctggct tcgcccgata acgcgatcca ttcgagtatt gctgctatgg
     8881 cactggaagc caatgttagc gaaccgacgg tgaatcgttt ctgtcgcagc atggacacgc
     8941 gcggttttcc tgattttaaa cttcatctgg cacagagtct ggcgaatggc actccctatg
     9001 ttaatcgcaa cgtcaatgaa gatgacagcg ttgaatcata cacagggaaa atatttgagt
     9061 ccgcaatggc aacgctcgat catgtccgtc attcactgga taaatctgcc atcaaccgcg
     9121 ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt cggattaggc tcttcagccg
     9181 ccgttgccca cgatgcgatg aataagttct ttcgttttaa tgttccggtg gtgtactccg
     9241 atgatattgt gctgcaacgc atgagttgta tgaattgtag cgacggagac gtggtggtgc
     9301 tgatttctca cactggaaga acaaaaaatc tggtcgagct ggcgcagctg gctcgcgaaa
     9361 acgacgcaat ggtgattgcc ctcacctctg cgggtacccc gctcgcccgg gaagcaacgc
     9421 tggcaattac cctcgacgta ccggaagata ctgacattta tatgcccatg gtttctcgac
     9481 ttgcacagct gaccgtgata gatgtgctgg cgacaggatt tactttgcga cgcggtgcaa
     9541 aattcagaga taacttgaag cgggtcaagg aagcgctgaa ggaatcgcgt tttgataagc
     9601 agttacttaa tttaagtgac gatcgctaaa aacgactgtc actgtcctaa tcttatacga
     9661 catccgaatg agattaattt atcgccatcg cggcgttatt tcattcggat ttcatgttca
     9721 agcaacacct ggttgtttca gtcaacggag tattacatgt ccagaaggct tcgcagaaca
     9781 aaaatcgtta ccacgttagg cccagcaaca gatcgcgata ataatcttga aaaagttatc
     9841 gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg gctcgcctga agatcacaaa
     9901 atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg ggcgtcatgt ggctattctg
     9961 ggtgacctcc aggggcccaa aatccgtgta tccaccttta aagaaggcaa agttttcctc
    10021 aatattgggg ataaattcct gctcgacgcc aacctgggta aaggtgaagg cgacaaagaa
    10081 aaagtcggta tcgactacaa aggcctgcct gctgacgtcg tgcctggtga catcctgctg
    10141 ctggacgatg gtcgcgtcca gttaaaagta ctggaagttc agggcatgaa agtgttcacc
    10201 gaagtcaccg tcggtggtcc cctctccaac aataaaggta tcaacaaact tggcggcggt
    10261 ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca ttaagactgc ggcgttgatt
    10321 ggcgtagatt acctggctgt ctccttccca cgctgtggcg aagatctgaa ctatgcccgt
    10381 cggctggcac gcgatgcagg atgtgatgcg aaaattgttg ccaaggttga acgtgcggaa
    10441 gccgtttgca gccaggatgc aatggatgac atcatcctcg cctctgacgt ggtaatggtt
    10501 gcacgtggcg acctcggtgt ggaaattggc gacccggaac tggtcggcat tcagaaagcg
    10561 ttaatccgtc gtgcgcgtca gctaaaccga gcggtaatca cggcgaccca gatgatggag
    10621 tcaatgatta ctaacccgat gccgacgcgt gcagaagtca tggacgtagc aaacgccgtt
    10681 ctggatggta ctgacgctgt gatgctgtct gcagaaactg ccgctgggca gtatccgtca
    10741 gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg aaaaaatccc gagcatcaac
    10801 gtttctaaac accgtctgga cgttcagttc gacaatgtgg aagaagctat tgccatgtca
    10861 gcaatgtacg cagctaacca cctgaaaggc gttacggcga tcattaccat gaccgaatcg
    10921 ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc taccaatttt cgccatgtcg
    10981 cgccatgaac gtacgctgaa cctgactgct ctctatcgtg gcgttacgcc ggtgcacttt
    11041 gatagcgcta atgacggcgt agcagctgcc agcgaagcgg ttaatctgct gcgcgataaa
    11101 ggttacttga tgtctggtga cctggtgatt gtcacccagg gcgacgtgat gagtaccgtg
    11161 ggttctacta ataccacgcg tattttaacg gtagagtaag tacgttgccg gatgcggcga
    11221 aaacgccaca tccggcctac agttcaatga tagttcaaca gatttcgaat attctgaagc
    11281 aaacttgaac ttatcatcag gcgaaggcct ctcctcgcga gaggcttttt tatttgatgg
    11341 gataaagatc tttgcgctta tacggctgga tttcgcccgg tttgcgagtt ttcagcaatt
    11401 ttagtatcca ggtgtattgt tctggtcgcg gaccaacaaa aatctcgact tcttcattca
    11461 tccgccgcgc aatcgtatga tcatccgcct ctaacagatc atccatcggt gggcgcacct
    11521 gaatcgtcag acggtgcgtc ttgccatcat aaatcggaaa cagcggaaca acgcgcgcac
    11581 ggcacacttt catcaaacga ccaatcgcgg gcaacgtcgc tttataggtg gcaaagaaat
    11641 caacaaattc gctgtgctca gggccatgat cctgatcggg taaataatat ccccagtagc
    11701 cctgacgtac cgactggatg aatggtttaa taccatcatt tctcgcatgc agacgaccgc
    11761 caaaacgacg acgcaccgtg ttccagacat aatcaaaaac cgggttgccc tgattatgga
    11821 acatcgctgc cattttctgc ccttgcgagg ccatcagcat ggcaggaata tcgacggccc
    11881 aaccgtgcgg caccagaaag ataactttct cgttattacg ccgcatctct tcaatgatct
    11941 ccagcccttg ccagtcaacg cgcggctgaa ttttctccgg cccgcgtatt gccaactcag
    12001 ccatcattgc catcgcttgc ggcgcggtgg caaacatctc atcaacaatc gcttcgcgtt
    12061 cagcttcact acgttctgga aagcagagcg acagattgat taacgcacga cggcgtgagc
    12121 tttttcccag tcgtccggca aaacgtccca gccgtgccag aatgggatca cggaactttg
    12181 gcggcgttaa agcgataccc gccatcgctg ctacgcccag ccatgctccc cagtagcgcg
    12241 ggtggcgaaa ggatttatca aactcaggaa tgtattcgct attatttttt ttcgtttcca
    12301 tgcttttcca gtttcggata aggcaaaaat caatctggtg atagtgtagc ggcgcaactt
    12361 gccccgcacc aaataaaaaa agccggtact gactgcgtac cggctgcgaa tggatgttaa
    12421 ttaatcaaac cgtagctgcg gcacaatctc tttggcctgc gccaggaatt cgcgacgatc
    12481 ggagccggtc agcccttcgg tacgcggcag ttttgccgtc agcgggttta cggcctgctg
    12541 gtttatccat acttcatagt gcagatgtgg cccggttgaa cgtccggtat taccggaaag
    12601 cgcgatacgg tcgccacgtt tcaccttctg tcccggtttc accagaatct tgcgcaagtg
    12661 catataacgc gtggtgtagc tgcgaccatg acgaatagcc acataataac ctgctgcgcc
    12721 actgcgtttg gcaaccacca cttcaccgtc acccactgaa agcactggcg taccctgcgg
    12781 catggcgaaa tcaacacctc tgtgtggtgc aacgcgaccg gtcacaggat tagtacgacg
    12841 cgggttaaag ttagacgaga tacggaactg tttcgccgtc gggaatcgca agaatccttt
    12901 cgccagacca gtaccgttac gatcgtagaa tttgccatct tcagcgcgga tagcgtaata
    12961 atctttacct tctgaacgca aacgtacgcc cagcagctgg ctttgctcac gtttaccatc
    13021 aagcatttct cgagacatta ataccgcaaa ttcatcgcct tttttcagtt tgcggaaatc
    13081 catttgccac tgcatggctt taatcactgc gctcacttcg gcgctggtta aaccggcgtt
    13141 tctggcgctg gcaacaaagc tccccccgac ggtacctttc agcagattgt taacccactc
    13201 tccttgctgc atttcgctgg tcattttaaa accgttagcg gcagtacggt cataggttcg
    13261 ggtttcacga cgagacactt cccaggtgag gcgctgcagt tcgccgtccg cggttaatgt
    13321 ccaggagagt tgttgaccga ttttcaggtt acgcaattct ttgtcggcag cagccagttg
    13381 ggtgatatca cccatatcaa taccatactg attgagaatg ctgcttagcg tatcgccagt
    13441 ggaaacaaca tattcatgca cgcccgcttc accggcgatt ttgtcatcca gttcgtcctg
    13501 gggaatggct tcatcttctt gtgcagcttg atcaatcggc tcactggctt caggtaagag
    13561 cgaacgaatt tcgttctgtt ccagctcaat ggttttgaca attggcgtgg catcgcggtg
    13621 ataaacatag ggccgccaga cagcgacggc cagagtaaga acggtgagcg accccaacat
    13681 aacgcggtgt ggtcgcggta aattattaaa cgccagggcg acagagcggg ctatctgttg
    13741 cacgtaatca cttcctcatt aatctccttt caggcagctc gcatactggt tggctaattg
    13801 actcaggaat tctgaatagc ttgttttacc cagtttgata ttcgtcccca ggggatccaa
    13861 cgttcccata cgaacggatg tccctcgtgc gacgctctca acgaccgctg gcctgaactg
    13921 tggctcagca aaaacgcagg ttgctttttg ctcaaccaac tgtgttctta tttcatgtaa
    13981 acgctgcgcg ccaggttgaa tctcagggtt aacggtaaaa tgaccaagcg gtgtcagtcc
    14041 aaactgtttt tcgaaatagc cgtaagcatc gtgaaaaacg aaataacctt tccccttgag
    14101 cggcgcgagc tcgttaccaa cctgcgtttc ggttgaggct aattgtgcct caaaatcctt
    14161 caggttggcg tcaagtttgg ctcgactttg cggcataagt tccactaatt ttccatggat
    14221 tgcaaccgct gtagcccgcg ctatctctgg ggaaagccaa agatgcatgt tgaaatcgcc
    14281 gtgatggtga tcttcgtcac ttttttccgc gtggtcgtga tcatcatcat cgccgtgaat
    14341 acttttcatc agtagcggtt tcacatcttc aagctgcgca atcgttacct gcttcgcttc
    14401 tggtaatttg cttaccggtt tttgcataaa cgcttccatc tccgggccaa cccaaacgac
    14461 taagtccgcg ttctgtaagc gttttacatc cgatggacgc agtgaataat catgttctga
    14521 agcgccgtca ggcagtaaaa cctccgtttc tgttacacca tcagcaatgg cagaagcgat
    14581 gaacccaacg ggtttaagcg aagcgacaac ggcggcatct gcggcctgtg ttgcacctcc
    14641 ccagagagcg gcggataatg ctgcgaaaag aagcgttttt ttatgtaaca taatgcgacc
    14701 aatcatcgta atgaatatga gaagtgtgat attataacat ttcatgacta ctgcaagact
    14761 aaaattaaca tgacaagtct ggtttccctg gaaaatgtct cggtttcttt tggccaacgc
    14821 cgcgtcctct ctgatgtgtc gctggaactt aaacctggaa aaattttgac tttacttggg
    14881 ccaaatggcg caggtaagtc gacactggta cgggtagtgc tcgggctggt aacacccgat
    14941 gaaggggtta tcaagcgcaa cggaaaactg cgcatcggct atgtaccgca gaagctgtat
    15001 ctcgacacca cgttgccact gaccgtaaac cgttttttac gcttacgccc tggtacacat
    15061 aaagaagata ttttgcctgc actgaaacgt gtccaggccg ggcacctgat taacgcaccg
    15121 atgcaaaagc tctctggtgg cgaaacgcag cgtgtactat tagcgcgagc attgttaaat
    15181 cgcccgcaat tattagtgct ggatgaaccc actcaaggcg tggatgtaaa tggccaggtg
    15241 gcgttatatg accttattga ccaactgcgt cgcgaactgg attgtggcgt tttaatggtc
    15301 tctcacgatc tgcatctggt aatggcaaaa accgatgaag tgctgtgtct gaatcaccac
    15361 atttgttgtt ccggcacacc ggaagttgtt tccctacatc cggagtttat ttctatgttt
    15421 ggtcctcgtg gtgctgaaca actgggtatc tatcgccatc atcataatca tcgtcacgat
    15481 ttacagggac gaattgtttt gcgtcgggga aatgatcgct catgattgaa ttattatttc
    15541 ccggttggtt agccgggatc atgctcgcct gtgccgcggg tccgctgggt tcgtttgtag
    15601 tctggcgtcg tatgtcttat ttcggtgata cgctggctca tgcctcatta cttggcgtcg
    15661 cgtttggttt gttgctggac gtgaatccat tctatgcggt aattgccgtt acgctgctcc
    15721 tggcgggcgg tctggtatgg ctggagaagc gtccacagct ggcgattgac acgttattag
    15781 ggattatggc gcacagtgcc ctgtcgctgg gcctggtggt cgttagtctg atgtctaaca
    15841 ttcgtgttga tttgatggct tacctgttcg gtgatttgct ggcagtgacg ccagaagatc
    15901 tcatcgctat tgcgattggc gtggtcatcg tggtggctat tttgttctgg caatggcgca
    15961 atttgctgtc gatgacgatt agcccggatc tggcgtttgt tgatggtgtg aaattacagc
    16021 gcgtgaaatt gttgttgatg ctggtgacgg cattgacgat tggtgtagcg atgaaatttg
    16081 ttggcgcgtt gattattact tcactgctga ttattcctgc agctactgcg cgtcgctttg
    16141 cccgtacgcc ggaacagatg gctggtgtcg ctgttttggt ggggatggtg gcagtaactg
    16201 gcggtttaac cttttccgcg ttttacgata cgccggcggg tccgtcggtg gtgctatgtg
    16261 cggcactgtt atttattctc agtatgatga aaaagcaggc cagctaatct atcgctgaac
    16321 acatttgtcg gatgcggcgc gagcgcctta tccgacctac ggttcgctat ctctggctgg
    16381 cctgataaga cgcgaacagt gtcgcatcag acacactgcc agtgtcggat gcggcgcgag
    16441 cgaccaatcc gacttacggc atttctggcg gcgttatgcc aaagtgattc cacgcccgcg
    16501 tcgtcgccat acgcccacgc ggtgtacgct gcaaaaagcc ttgctgaatc aaataaggtt
    16561 ccagcacatc ctcaatggtt tcacgttctt cgccaatggc tgccgcaagg ttatccagac
    16621 ctaccggccc accaaagaac ttatcgatta ccgccagcaa taacttgcgg tccatataat
    16681 cgaaaccttc agcatcgaca ttcaacatat ccaacgcctg agcagcgata tctgccgaga
    16741 tggtgccatc gtgcttcact tcggcgaaat cacgcactcg acgcagcaga cggttggcaa
    16801 tgcgcggcgt accgcgagct cgacgagcaa cttccagcgc gccgtcatca ctcatctcaa
    16861 gccccataaa gcgtgcgctg cgactgacga tatattgcag atccggcacc tgataaaact
    16921 ccagacgctg cacaatacca aaacgatcgc gcaacggtga tgtcagcgaa cctgcgcgcg
    16981 tggttgcacc aatcagggta aacggcggca aatcaatttt aatggagcgt gccgccggac
    17041 cttcaccaat catgatatcc agttggtagt cttccattgc cggatacaac acctcttcca
    17101 ccactggcga aagacggtgg atctcatcaa taaacaacac gtcatgcggt tcaaggttag
    17161 tgagcatcgc tgccagatcg cccgcctttt ccagcaccgg accagaagtc gtgcgtaaat
    17221 taacgcccat ttcattggcg acgatgttgg caagcgtagt tttacccaac cccggaggac
    17281 cgaaaatcaa cagatgatcg agggcatcgc cgcgcagttt cgctgctttg atgaaaatct
    17341 ccatctgtga acgaacctgc ggctgaccaa catactcttc cagtaattta gggcgaatgg
    17401 cgcgatctgc cacatcttcc ggcaaagtgg taccggcaga aatcagacgg tctgcttcaa
    17461 tcatccttta cctcataacg cggcgcgtag ggcttcgcga attaatgttt cactgctggc
    17521 gtcagggcga gcgattttgc tcaccatgcg gcttgcctct tgtggtttat agcccagcgc
    17581 caccagcgcg gcaactgctt cttgttcagc atcgtcggtc gccgggctgg caggtgatgt
    17641 gagtaccagg tcggcggctg gcgtaaagag atcgccatgc aaacctttaa atcggtcttt
    17701 catttcaaca atcaagcgtt cggcggtttt tttgccaata cccggcagtt tcaccagcgc
    17761 ccccacttct tcacgctcaa cggcattaac gaactgctgc gctgacattc cggagaggat
    17821 cgccagcgcc aacttcgggc cgacgccgtt ggttttgatc aactctttga acaatgtgcg
    17881 ctcttgttta ttgttaaaac cgtacagcag ttgcgcgtct tcacgcacca caaagtgggt
    17941 gaaaacgatc gcttcctgac ccgcttcagg gagttcataa aaacaggtca tcggcatatg
    18001 cacttcatag cctacgccgc ccacttcaat taacaccagc gggggttgtt tttcaatgat
    18061 gatgcctctg agtctgccta tcacatgacg ctcctgcgta atgaatcaaa gataatgctg
    18121 tatgataaaa aaatgctgga tagatatcca gcgaaggatg aagaaaactt gcgaggtgtc
    18181 ccgatgatct gaaaaatggc agagtataat ttattcaaca ggttatattg gaagcaaata
    18241 ttttaatatt acatattcag tgaagaaatg cgtaataaaa atatacattg cgactcctga
    18301 aaaaataaat tttttatgct attacgtata ttcgtatcga tttcaatgga atgacaacgt
    18361 gaatattaat taccctgctg aatatgaaat tggagatatc gtctttacat gtataggtgc
    18421 tgccttattt ggtcaaatat cagctgcatc aaattgctgg agtaatcacg tcgggatcat
    18481 tatcggtcat aacggtgaag acttcctggt tgcagaaagc cgggttcccc tttcaaccat
    18541 caccacgcta tctcgtttta ttaaacgttc gtctaatcaa cgctatgcta taaagcgatt
    18601 agacgctggg ctgacagaac aacaaaaaca acgaattgtt gaacaggttc cttcccggct
    18661 acgcaaactt taccacacgg gttttaaata cgaatcttcc cgccagttct gttcaaaatt
    18721 tgtctttgat atttataaag aggcgctatg tattccggtg ggtgaaatag agacgtttgg
    18781 acaactgtta aatagcaatc cgaatgcaaa actcactttc tggaaattct ggttcttagg
    18841 ttctattccg tgggagcgta aaaccgtcac gccagccagt ttgtggcatc atccgggttt
    18901 ggtgttgatt cacgcggagg gagttgaaac gcctcagcct gaactgaccg aggcggtata
    18961 acttaacgca gtcgccctct cgccaggttc agccgcgatt cgctcatctg catcgcattc
    19021 tgactgacgt gacaatgggt aatcgcgata gctaacgcat cggcggcatc cgcctgtgga
    19081 ttagcgggca gtttcagcaa ggtgcggacc atatgctgca cctggctttt ttcggcacta
    19141 ccaataccta ccaccgtttg ctttacctga cgtgccgcgt attcaaatac cggcaattcc
    19201 tgattcaccg ccgcaacaat cgccacgccg cgcgcctggc ccagtttcag ggctgagtca
    19261 gcgttcttcg ccataaagac ttgttcaatg gcgaaataat caggctggaa ctgggtgatg
    19321 atttccgtca cgcccgcata gatgagcttc agacgagacg gtaaatcatc cactttggtg
    19381 cgtatgcatc cgctacccag gtaggacagt tgcctgccta cctggcgaat tacgccatag
    19441 ccggtcacgc gcgaacccgg atcaatgccg agaataatag ccatcacgcg tctccgtttt
    19501 gctgtttagc aggcctcatc agagagtcgc tgcgacctca tcagagattt caccgttatg
    19561 gtaaacttcc tgcacgtcgt cgcaatcttc cagcatatcg atcagacgca tcagtttcgg
    19621 tgcggtttct gcatccatat cagctttggt ggacgggatc atggaaactt ccgcgctgtc
    19681 tgctttcaga cctgccgctt ccagagcgtc gcgcacttta cccatttctt cccatgcagt
    19741 gtagacatca atcgcgccgt catcataggt cacaacgtct tcagcacctg cttccagagc
    19801 tgcttccatg atggtgtctt catcgccttt ctcgaaggag atcacgcctt ttttgctgaa
    19861 cagataggct acggaaccat ctgtaccgag gttaccgcca catttgctga atgcatgacg
    19921 cacttcagca acggtacggt tgcggttgtc agacagacat tcaatcatga ttgccgtgcc
    19981 gccaggaccg taaccttcgt agatgatggt ttccatgttt gcatcatcat caccgcccac
    20041 accacgtgca attgcgcggt tcagtgtgtc gcgggtcatg ttgttagaca gtgctttatc
    20101 aattgctgca cgcagacgcg ggttagcgtc cggatcgcca ccgcccagct tagccgcggt
    20161 taccagctca cgaatgattt tagtgaagat tttaccgcgc tttgcatcct gcgcagcttt
    20221 acgatgtctg gtgttggccc atttactatg acctgccata aaaatatctc cagatagccc
    20281 tgcctgttca ggcagcgtta attacaaact gttcaatcgc ctgccggttg ctccaggact
    20341 tagtgagcgc cgccgcagca ggcgcatcaa gccacttgta agccagatgt tcagtgaaaa
    20401 cgatctgccg ctcgtgcgga agcgcaagac agaaccatga ttccgtatta cgcgtcacgc
    20461 ccggcgcata gcgatgacgt aaatgtgaaa aaatttcaaa ctctaccgtg cgctgacagt
    20521 caattaaggt cagttgttca gcgacaacat caatggtgac ctcttccttt acttcgcgca
    20581 tggcagcttg cggcgcggtt tcaccctctt ccacgctgcc ggttaccgac tgccagaaat
    20641 cgggatcgtc acgccgctgc aacatcagca cccgtttcgt atcttgtgca tagatgacca
    20701 ctaagatcga aacgggacgc ttatacactt tatccttcac gctgcctctt cgttgactgc
    20761 cttcgctcat cccattcaca tagttatcta tgctcatggg agttcactca gttgccgcct
    20821 tgatgcaacg cgaatgattt cgtgtatttg agtcatatca gttattctca gccttcttca
    20881 caacctgaat gctcagctca gccagtgcag tcgggttagc aaagctcggt gcttcagtca
    20941 tcagacacgc tgccgccgtg gttttcggga aggcgataac gtcacggata ttgtcggtgc
    21001 cagtcagcag catggtcaga cggtcaagac cgaatgccag acctgcgtgc ggcggagtac
    21061 cgtatttcag ggcgtcgagc aggaagccga atttctcgcg ctgttcctct tcgttgatac
    21121 ccagaatacc aaacaccgtc tgctgcatat caccattatg gatacgtact gacccaccgc
    21181 ccacttcgta accattgatg accatatcgt aagcgttcgc caccgcattt tccggtgcag
    21241 ctttcagttc tgcagcagtc atgtctttcg gtgaggtgaa cggatggtgc attgccgtca
    21301 ggccgccttc accgtcgtct tcaaacatcg ggaagtcgat aacccacagc ggtgcccatt
    21361 tgctttcgtc ggtcagacca aggtctttac ccactttcag gcgcagtgcg cccatcgcgt
    21421 cggcaacaat tttcttgttg tcggcaccga agaaaatcat atcgccatct tgcgcggcag
    21481 tacgatccag gatggcttcg atgatttctg cattaaggaa cttcgctacc gggctgttga
    21541 taccttccag acctttcgcg cgttcgttaa ctttgatgta agccagacct ttcgcgccgt
    21601 agattttaac gaagttaccg tattcgtcga tctgcttacg ggtcagcgat gcgccacccg
    21661 gaacacgtag agcggcaaca cggcctttcg gatcgttcgc cggccccgca aatactgcaa
    21721 actcaacaga tttcagcaga tcggcaacgt cggtcagttc catcgggtta cgcagatccg
    21781 gtttatcaga accataacgg cgttctgctt ccgcaaaggt cattaccggg aaatcgccca
    21841 gatccacgcc cttcacttcc agccacagat ggcgcaccag cgcttccatc acttcacgca
    21901 cttgcggcgc ggtcatgaaa gaagtttcca catcgatctg agtaaattca ggctgacgat
    21961 cagcgcgcag gtcttcgtca cggaagcatt taacgatctg atagtagcgg tcaaagccgg
    22021 acatcatcag cagctgtttg aacaactgcg gggattgtgg cagcgcgtag aatttacctt
    22081 tgtgcacacg agaaggcacc aggtagtcac gcgcgccttc cggcgtggct ttggtcagca
    22141 tcggagtttc gatgtcgagg aagccgtggt catccataaa acggcgcacc aggctggtga
    22201 ttttagcgcg ggttttcagg cgctgagcca tttccgggcg acgcaggtcg aggtagcggt
    22261 atttcagacg cgcttcttcg gtgttgacgt ggttagagtc aagcggcaga acatctgcgc
    22321 ggttgatgat agtcagcgag gacgccagca cttcgatttc gccggtcgcc atatcgcggt
    22381 taatattttt ttcgtcacgc gcacgtacgg tgcccgtgac ctgaatgcag aactcattac
    22441 gcagttcaga ggccagcttt aacgcgtccg cacgatccgg atcgaaaaat acctgcacga
    22501 taccttcgcg gtcgcgcata tcgatgaaga tcaggctacc aagatcacga cgacggttga
    22561 cccaaccaca cagagtcacc tgctgcccca cgtgggacaa acggagctgt ccacaatatt
    22621 ctgtacgcat gagatatccc ttaacttagc tgccggcgga tgccccctgc tgcgcaggtg
    22681 accaagtcgc agcgttagct gtatgtcaca actgaatgaa aaaaggcggc tattatactg
    22741 gaaattctgc cgcaccgtaa gagcctggcc cgcgctggaa cgcctcgtta ccactttata
    22801 tcgggcctga aatcagactc tacgccagtt tgctataaag gtgttgcccg aactcataaa
    22861 aattaacaaa atttgtcgtt ccgccatcgg ctaatcgcat taaggtgtga ggcacgattt
    22921 tgttttttca ggagtcagca tgcttgaact taatgctaaa accactgcgt tggtggtgat
    22981 cgatttacaa gaaggcatct taccttttgc cggtggtcca catactgccg atgaggtagt
    23041 taatcgcgcc gggaagctgg cggcgaaatt tcgcgccagc ggtcagcccg tgtttctggt
    23101 gcgagttggc tggtctgccg attacgccga agcattaaaa cagccggttg atgccccctc
    23161 ccccgctaaa gtgttgcccg aaaactggtg gcaacatcca gctgcattag gtgcaactga
    23221 cagtgatatc gaaatcatca aacgtcagtg gggtgcgttt tacggtacgg atctggagtt
    23281 gcaattacgc cgccgaggta tcgatacaat agtgttatgt gggatctcga ccaatatcgg
    23341 tgttgaatcc accgcccgca atgcctggga acttggcttt aatctggtga ttgccgaaga
    23401 cgcctgtagt gccgccagcg ccgagcagca caataacagc atcaaccata tctacccacg
    23461 catcgcccgt gtgcgtagcg tggaagagat ccttcacgcg ttatgattta catcggtcta
    23521 ccgcaatggt cgcatcctaa atgggtgcgg ttggggatca ccagccttga agagtatgcc
    23581 cgccacttta actgcgtgga gggcaacacc acgctttacg ccctgccgaa acccgaggtt
    23641 gtcctgcgct ggcgtgaaca gaccacagat gacttccgct tctgttttaa gtttccggcg
    23701 accatttcgc atcaggcagc attacgacat tgcgatgatt tagtgactga atttttgacc
    23761 cgcatgtcac cgttggctcc gcgcattggg caatactggc tgcaactgcc tgccacattc
    23821 ggcccacggg agctgcctgc gctttggcat tttctcgatt ctctccctgg cgaatttaat
    23881 tatggtgtgg aagtccgcca tccacagttt ttcgccaaag gggaagagga acaaacgctt
    23941 aatcgcggtt tacatcagcg cggcgttaat cgggtgattt tagacagccg cccggttcat
    24001 gcagcacgtc cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg
    24061 gtacatgctg tactgacggc gacaaatccg ctgatccgtt ttatcggtag tgatgatatg
    24121 acgcaaaacc gggaattatt tcaggtctgg ttacaaaaat tagcgcagtg gcatcagacc
    24181 actacgcctt atcttttttt acatacgcca gacattgccc aggccccgga actggtacat
    24241 accctgtggg aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca
    24301 cagcaatctt ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt
    24361 aaagggagtt tgtatggtaa gcgcgctgta tgccgtttta agtgcgttgt tattaatgaa
    24421 gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg
    24481 ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat atattcccat
    24541 cgcgattgtg ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat
    24601 ttgcggcatc gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct
    24661 gtttcgctgg cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct
    24721 ggcgaatctt tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg
    24781 ccactttctt tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc
    24841 ctatcgccag actgggcgac tggacctttg atgaacgggt agctgaagtc ttcccggata
    24901 tgatccagcg ttccgttccc ggctattcca atattatttc catgattggt atgttagccg
    24961 agcgcttcgt tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga
    25021 cgctctcggt gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact
    25081 ccccggcgat gattgaacgc tgccgtcgtc atattgacgc ctataaagcc cctacgccag
    25141 tagacgtcat tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc
    25201 tgaattttac cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt
    25261 atcaaggact gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg
    25321 ccaaagttgg tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca
    25381 gcgaactgga gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg
    25441 tggaaaccca taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc
    25501 agtgctttaa ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt
    25561 tggtaacttt tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc
    25621 cgcgcagatt gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa
    25681 cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac
    25741 cgccgaaagc gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg
    25801 gaacctgatg ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga
    25861 atggcgttcc gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg
    25921 caccattctg gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg
    25981 cgcgcacctc gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt
    26041 gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct
    26101 tccggcactg aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc
    26161 gccgctggag catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct
    26221 ggaaacgctg gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc
    26281 gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa
    26341 gtgtggtttt gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg
    26401 acgcactgaa tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag
    26461 taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta
    26521 aaggtctggt attactgccg gatgcggcgt gaatgcctta tccggcctac aaacccttgc
    26581 taattcaatc tattgcaggg acta
//
LOCUS       DAFATA010000058        26157 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFATA010000058 DAFATA010000000
VERSION     DAFATA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.059"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="LGJ20_002886"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="LGJ20_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBK2386920.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="LGJ20_002887"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="LGJ20_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBK2386921.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="LGJ20_002888"
     CDS             complement(941..1408)
                     /locus_tag="LGJ20_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK2386922.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="LGJ20_002889"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="LGJ20_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK2386923.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="LGJ20_002890"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="LGJ20_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK2386924.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="LGJ20_002891"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="LGJ20_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK2386925.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="LGJ20_002892"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="LGJ20_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK2386926.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="LGJ20_002893"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="LGJ20_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK2386927.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="LGJ20_002894"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="LGJ20_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK2386928.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="LGJ20_002895"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="LGJ20_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK2386929.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="LGJ20_002896"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="LGJ20_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK2386930.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="LGJ20_002897"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="LGJ20_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK2386931.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="LGJ20_002898"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="LGJ20_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK2386932.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="LGJ20_002899"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="LGJ20_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK2386933.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="LGJ20_002900"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="LGJ20_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK2386934.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="LGJ20_002901"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="LGJ20_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK2386935.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="LGJ20_002902"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="LGJ20_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK2386936.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="LGJ20_002903"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="LGJ20_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK2386937.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="LGJ20_002904"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="LGJ20_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK2386938.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="LGJ20_002905"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="LGJ20_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK2386939.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="LGJ20_002906"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="LGJ20_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK2386940.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="LGJ20_002907"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="LGJ20_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK2386941.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="LGJ20_002908"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="LGJ20_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK2386942.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="LGJ20_002909"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="LGJ20_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK2386943.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="LGJ20_002910"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="LGJ20_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK2386944.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="LGJ20_002911"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="LGJ20_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK2386945.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="LGJ20_002912"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="LGJ20_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK2386946.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="LGJ20_002913"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="LGJ20_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK2386947.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="LGJ20_002914"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="LGJ20_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK2386948.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="LGJ20_002915"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="LGJ20_002915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK2386949.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="LGJ20_002916"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="LGJ20_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK2386950.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="LGJ20_002917"
     CDS             15548..15916
                     /locus_tag="LGJ20_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBK2386951.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNGGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="LGJ20_002918"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="LGJ20_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBK2386952.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="LGJ20_002919"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="LGJ20_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBK2386953.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="LGJ20_002920"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="LGJ20_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK2386954.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="LGJ20_002921"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="LGJ20_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBK2386955.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="LGJ20_002922"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="LGJ20_002922"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK2386956.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="LGJ20_002923"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="LGJ20_002923"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK2386957.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="LGJ20_002924"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="LGJ20_002924"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK2386958.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="LGJ20_002925"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="LGJ20_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK2386959.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /locus_tag="LGJ20_002926"
     CDS             22593..23066
                     /locus_tag="LGJ20_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBK2386960.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="LGJ20_002927"
     CDS             23095..23637
                     /locus_tag="LGJ20_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBK2386961.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="LGJ20_002928"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="LGJ20_002928"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBK2386962.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="LGJ20_002929"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="LGJ20_002929"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK2386963.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="LGJ20_002930"
     CDS             25034..25291
                     /locus_tag="LGJ20_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBK2386964.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="LGJ20_002931"
     CDS             complement(25267..25821)
                     /locus_tag="LGJ20_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK2386965.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6717 a         6207 c         6786 g         6447 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggtaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatggcggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgttc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actggcagga ttacactcgc
    16381 gccgtaaaat aaccaattgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg cgtagagaaa
    16501 aataagaagg ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gtcttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgccag taatacttct
    16681 tctttagttg gtgcaggtgc ggcagctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttctggtg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacggtta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tctgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccttg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ttatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag aaaaaatcct gaaattcagg gttgactctg aaagaggaaa
    26101 gcgtaatata cgccacctcg cgacagtgcg ctaaagcgcg tcgcaactgc tctttaa
//
LOCUS       DAFATA010000059        26038 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFATA010000059 DAFATA010000000
VERSION     DAFATA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.060"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..899)
                     /locus_tag="LGJ20_002932"
     CDS             complement(<1..899)
                     /locus_tag="LGJ20_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBK2386966.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWG"
     gene            complement(1172..2275)
                     /gene="asd"
                     /locus_tag="LGJ20_002933"
     CDS             complement(1172..2275)
                     /gene="asd"
                     /locus_tag="LGJ20_002933"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK2386967.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            2468..3061
                     /gene="yhgN"
                     /locus_tag="LGJ20_002934"
     CDS             2468..3061
                     /gene="yhgN"
                     /locus_tag="LGJ20_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBK2386968.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(3118..4458)
                     /gene="gntU"
                     /locus_tag="LGJ20_002935"
     CDS             complement(3118..4458)
                     /gene="gntU"
                     /locus_tag="LGJ20_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBK2386969.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(4462..4989)
                     /gene="gntK"
                     /locus_tag="LGJ20_002936"
     CDS             complement(4462..4989)
                     /gene="gntK"
                     /locus_tag="LGJ20_002936"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK2386970.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(5128..6123)
                     /gene="gntR"
                     /locus_tag="LGJ20_002937"
     CDS             complement(5128..6123)
                     /gene="gntR"
                     /locus_tag="LGJ20_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBK2386971.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(6347..7042)
                     /gene="yhhW"
                     /locus_tag="LGJ20_002938"
     CDS             complement(6347..7042)
                     /gene="yhhW"
                     /locus_tag="LGJ20_002938"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBK2386972.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(7165..8202)
                     /locus_tag="LGJ20_002939"
     CDS             complement(7165..8202)
                     /locus_tag="LGJ20_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK2386973.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            8535..9023
                     /gene="yhhY"
                     /locus_tag="LGJ20_002940"
     CDS             8535..9023
                     /gene="yhhY"
                     /locus_tag="LGJ20_002940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2386974.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            9257..10435
                     /locus_tag="LGJ20_002941"
     CDS             9257..10435
                     /locus_tag="LGJ20_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HBK2386975.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRWEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAILSGSIYPPMKNIMDHTK
                     "
     gene            10432..10926
                     /locus_tag="LGJ20_002942"
     CDS             10432..10926
                     /locus_tag="LGJ20_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386976.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
     gene            11021..11176
                     /locus_tag="LGJ20_002943"
     CDS             11021..11176
                     /locus_tag="LGJ20_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2386977.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            11376..11660
                     /locus_tag="LGJ20_002944"
     CDS             11376..11660
                     /locus_tag="LGJ20_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HBK2386978.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(11698..13440)
                     /gene="ggt"
                     /locus_tag="LGJ20_002945"
     CDS             complement(11698..13440)
                     /gene="ggt"
                     /locus_tag="LGJ20_002945"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBK2386979.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            13560..14000
                     /locus_tag="LGJ20_002946"
     CDS             13560..14000
                     /locus_tag="LGJ20_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBK2386980.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(13987..14730)
                     /gene="ugpQ"
                     /locus_tag="LGJ20_002947"
     CDS             complement(13987..14730)
                     /gene="ugpQ"
                     /locus_tag="LGJ20_002947"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK2386981.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGESLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(14727..15797)
                     /gene="ugpC"
                     /locus_tag="LGJ20_002948"
     CDS             complement(14727..15797)
                     /gene="ugpC"
                     /locus_tag="LGJ20_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBK2386982.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(15799..16644)
                     /gene="ugpE"
                     /locus_tag="LGJ20_002949"
     CDS             complement(15799..16644)
                     /gene="ugpE"
                     /locus_tag="LGJ20_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBK2386983.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTYLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(16641..17528)
                     /gene="ugpA"
                     /locus_tag="LGJ20_002950"
     CDS             complement(16641..17528)
                     /gene="ugpA"
                     /locus_tag="LGJ20_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBK2386984.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(17626..18942)
                     /gene="ugpB"
                     /locus_tag="LGJ20_002951"
     CDS             complement(17626..18942)
                     /gene="ugpB"
                     /locus_tag="LGJ20_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBK2386985.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(19338..20051)
                     /gene="livF"
                     /locus_tag="LGJ20_002952"
     CDS             complement(19338..20051)
                     /gene="livF"
                     /locus_tag="LGJ20_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBK2386986.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(20053..20820)
                     /gene="livG"
                     /locus_tag="LGJ20_002953"
     CDS             complement(20053..20820)
                     /gene="livG"
                     /locus_tag="LGJ20_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBK2386987.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(20817..22094)
                     /gene="livM"
                     /locus_tag="LGJ20_002954"
     CDS             complement(20817..22094)
                     /gene="livM"
                     /locus_tag="LGJ20_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBK2386988.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(22091..23017)
                     /gene="livH"
                     /locus_tag="LGJ20_002955"
     CDS             complement(22091..23017)
                     /gene="livH"
                     /locus_tag="LGJ20_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBK2386989.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(23065..24174)
                     /gene="livK"
                     /locus_tag="LGJ20_002956"
     CDS             complement(23065..24174)
                     /gene="livK"
                     /locus_tag="LGJ20_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBK2386990.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            24598..24981
                     /gene="panM"
                     /locus_tag="LGJ20_002957"
     CDS             24598..24981
                     /gene="panM"
                     /locus_tag="LGJ20_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBK2386991.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(24978..25505)
                     /locus_tag="LGJ20_002958"
     CDS             complement(24978..25505)
                     /locus_tag="LGJ20_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2386992.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(25512..25778)
                     /locus_tag="LGJ20_002959"
     CDS             complement(25512..25778)
                     /locus_tag="LGJ20_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBK2386993.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
BASE COUNT         6606 a         6939 c         6340 g         6153 t
ORIGIN      
        1 taaccccaac tgccatcgaa gggatgctcg ttaatgggca gtagttcgag gtgggtaaag
       61 cccatccatt tagcataagg caccagttga tcggccagct cgcggtagct caaccagaaa
      121 ttgttgtcgg tgtggcgacg ccaggaaccc aggtgaactt cataaataga gattggcgca
      181 tcaaactgat tcgctttttt gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa
      241 ataagagacg cggtttccgg gcgcatttgc gcttcgaagg cataagggtc ggacttcaga
      301 cgcaagttgc cattggcatc aatcatctcg tatttataga gctgaccgtt atgcgcccca
      361 gggataaaca gttcccagat gccgctctct ttacgcaggc gcatcgggtg acggcgaccg
      421 tcccagtagt tgaattgccc aaccaccgag acccgacggg cgtttggagc ccagacagag
      481 aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg cgcctaaggt ttcatacggg
      541 cgcaggtgag taccttcaga taatagccag gcatccattt cctggattag cggaccaaaa
      601 cggtaaggat catcaatcag gttttgctga ccatgccaga caacagccaa ctgatagcgg
      661 aaaaaattct tacgtcgcgg aatgacgccg ctaaagaatc cccgtgagtc gagacactcc
      721 agttttgcga gtttgcgccc ggttttcggt tcaatcaccc acacatcggt agcgtcgggt
      781 aaaagggcac ggacttccag tcccgcggtg gttttatgca ttcccagtac ggaaaaagga
      841 tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc tatcgatacg atcggacatg
      901 cttgtcttcc tgttttattg tgtcacccca tccaattgga tttttgactt ctggttgtga
      961 catttttttg acctgaacgg cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc
     1021 aggtaaggct gtgaatactc atgtattcag ccacccttaa agaatagcca atgctctatt
     1081 taactcccgg taaatcatga aacatctgcg cttactcctg tattacgcac taacaggggc
     1141 ggcatcgcgc cccagattta atgaataaag attacgccag ttgacgaagc atccgacgca
     1201 gcggctccgc ggccccccac agcagctggt cgcccacggt aaaggctgac aggaactctg
     1261 gtcccatatt cagcttacgc aggcggccta ccggcgtggt cagcgtgccg gtaacggcag
     1321 ctggggttag ctcacgcata gtgatttccc gatcgttcgg aacgactttc gcccacggat
     1381 tgtgcgcagc cagcagttct tccacggtcg gaatagacac atcttttttc aatttaatag
     1441 tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac acataaacca tctaccggaa
     1501 ttacggaaga tgtgttgagg atcttgttgg tttccgcctg ccctttccac tcttcgcggc
     1561 tctgaccgtt atcgagctgt ttgtcgatcc acggaatcag gctacccgcc agcggcacgc
     1621 caaagttatc caccggcagc tcaccgctac gggttaaggt tgtgactttg cgttcgatat
     1681 cgagaatagc agaggacggg gtcgcgagtt catctgccac atggccatac agatggccca
     1741 tctgggttaa taactcacgc atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg
     1801 caacggacac ccaatcaaca agatcattgg cgaataaacc acccaacgac atcaacatca
     1861 ggcttacggt acagttaccg ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa
     1921 tgacgtcctg attgacgggg tcaagaatga tgatggcgtc atctttcatg cgcagagacg
     1981 atgctgcgtc aatccagtaa ccttgccatc cgctttcacg aagctttgga tagatttcgt
     2041 tggtataatc gccgccctga caggtcacaa tgatatcgag ggcctttagc gcctccagat
     2101 caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga cggcgcagcc tggccaagct
     2161 gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt
     2221 gcatgagaac ggagccgacc ataccgcgcc agccgataaa accaacattt ttcataagcg
     2281 tttttttcct gcaaagatgt gtgctgtata aatgtgccgg tctcctcttg gcacatcttt
     2341 caccatacaa aaagcagcca aagtcgcaag tgaaattaat caatgatagc gaagccatca
     2401 gtaatgcgac ttatcctgct ttgttagcac gcagaaagtc cgcggcaatt atcagggaat
     2461 ttgagttatg aatgaaatca tttctgcagc agttttattg atcctgatta tggatccgct
     2521 cggaaaccta cctattttca tgtccgtact gaaacatact gaaccgaaaa gacggcgggc
     2581 aatcatggtg cgagagttgc ttattgctct cctggtgatg ctggtgttcc tgtttgcggg
     2641 tgagaaaatt ctggcatttc tcagcctgcg agcagaaacc gtctccattt ctggcggcat
     2701 cattctgttt ctaattgcca ttaagatgat tttccccagc gcttcaggaa atagcagcgg
     2761 gcttccggca ggtgaagagc catttatcgt gccgttggca attccgttag tcgccgggcc
     2821 gactattctc gccacgctga tgttgttgtc tcatcagtac ccgaatcaga tggggcatct
     2881 ggtgattgct ctgctgctgg cctggggcgg cacctttgtc atcctgctac agtcttcgct
     2941 atttttacgt ctgctgggcg agaaaggggt gaacgcactt gaacgcctga tgggattgat
     3001 tctggtgatg atggcaaccc agatgttcct cgacggcatt cgaatgtgga tgaaggggta
     3061 acgaggagag tttttcgaaa agaacccggt agtgtcacaa ctaccgggcg aacaaactta
     3121 actcaacagc tggaacgcaa tcatcccaac gatggcaccg acagtgccga ggatggtttc
     3181 catcatggtc caggttttca gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa
     3241 ccagaaaccg gcgtcgttaa cgtggctgac aacaatcgaa ccaccagcga tacaaatcga
     3301 cagcgccgcc atttgcgcac cggagtagtt cagttgttca ataaccggca tcaccagtcc
     3361 taccgccgtt aaacaggcta cggtggcaga accctgaatg atgcgcactg cagctgccag
     3421 cacgaagcag gtgatagcaa tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg
     3481 acctacgcca gagtcaacca gcacctgttt gaacacgccg cctgcaccaa tcaccagcag
     3541 aatgatcccc gccggttgca gcgcgtgacc gcaaatctcc atcactttgt cttttggcat
     3601 gccctgacgc attgccaggc cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa
     3661 cggatgacca ataaactcga accattcgta agcggtagag ccttctggca caaaacgcgc
     3721 ggcaatggtt ttcagcccca ccagcaccag cggcaacagg atcagcgaca ggctgaatcc
     3781 gaaagatggc attttgcctt cgccgagatg cggttcgctg atgtcgtcag gaatatgcaa
     3841 ctccacgtag cggctgatga aattacccca cagcggcccg gcaataatca ttcccggaat
     3901 tgccgcacac aggccaatca ggatcatcca gccaaaatcg gcgttcatct gcgatgcgag
     3961 gagcattggc gctggcccag gcaccaggaa cgcagccgcg gccgccacgc ctgcgaataa
     4021 tgggattacc agcttcacca ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct
     4081 aatcagcaga acaatcgcca cttcaaagaa cagcggtagc gcacagacca gccccgcaag
     4141 gccgatggca taatgcgcgc ggctgtgacc gaaggatttg agcattttga cggcaatctg
     4201 atcgactgcg ccggtttcat gtaagatctt gccaaacata gctcccaggg cgacaaccac
     4261 cgccaggaag ccgagggtgc ctcccatccc tttttccatc gtcgctgcga ttttatcgag
     4321 cggcataccg gaaaaaaggc cagcccccat ggacaccacc atcaaagcca ggaaagcgtg
     4381 catacgcgcc ttcatgacta aaaacagcag cagtaaaaca gaccctactg ctgttaaaac
     4441 aagcgttaat gtagtcacta cttatttgcc ttttttaata acctcaatgg tgcttgccac
     4501 aacaccttcc agcggttgat cgatatccac caccagtaca tcagtttcgt ccgcacccgg
     4561 ctcctgcagc gtttcaaact gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg
     4621 cgctttcagg cggctttcaa tcacgtcaaa atcgcctttc aggtagatga aagagagatt
     4681 cggattacct tcacgcagca agtcgcgata gtgttttttc aatgcagaac agacgatcag
     4741 tgacacttta ttagtgcgct gcatagcaaa cgcggcgtcg ttcagcgcct gcaaccacgg
     4801 tttgcgatcg tcgtcattca gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg
     4861 atggaggaaa tcgccatcaa gaaacgcggc atgaagttga tgcgccactt cactggcgac
     4921 cgcagatttg ccgctgcctg agacgcccat caagacgtaa atgtggtgat catggttagt
     4981 cgtgctcaaa gtggtgcccc cacaatacaa gaattaaaaa tgttacgggt aactgttatc
     5041 ggtaacattg tccggccgga caatatcaga agcaatatcc atccgtgcct taagtgtata
     5101 agtgtgagct acttcaaatt tgtgggctta aatagatccg cccggtgaca aggtgaaacc
     5161 taaatctaac attttcggtg tcacagattc gccacgaata cgcgccagca ggcgttcagc
     5221 gccaatactg cccatccgct cacgcggcgt cagcacgctc gccagtcgtg gctccatcac
     5281 ctgaccaatg tcatgaccgt ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc
     5341 cagacgctga cattcaaacg ccgcgccgac cgccagatca tcattagtac agaacacgcc
     5401 atccagctgc ggatattccc gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga
     5461 agaagattgc tcaaccatca cgctatacgg caccaggcct gcatccagca tcgcctgttc
     5521 gtatcccttc tgtttgatga tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg
     5581 gcgatgcccg cgcgcaataa tggcggtagt catctggcgt gccgcttcaa agttatcaaa
     5641 accgacggcg atatcgaggc acggcgactg gctgtccatc aactccacca ccgggatccc
     5701 cgccacttca atcatcttta aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc
     5761 gtcgatattc caggacagca tggattcgag acgttcttgc tccatttccg gtttataacc
     5821 gtagtgcgcc agcatcgtct gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa
     5881 tacttccgcg aaaacctggt tggtgagaga aggtaacagg acgccaatcg cccggctggt
     5941 agcgttagag aggatatcgg gcgcacgatt gggaatatag cccagttcat caagagcggc
     6001 ggcaatcttg ccgcgtagag cgacggaaac ctgctccggg ttacgcaaaa agcggctgac
     6061 cgtcattttg gtcacgccta cacggtcagc cacatcctga agtacgggtc ttttcttttt
     6121 catcgtcctg aaggtacaaa agagatagat tctcttagtt taacacggac gcatcagaac
     6181 cttcccgggg aaaacagtgc ttgtttacaa ttattagata tacactaaat caattgcatc
     6241 gcattgtgct aacgacgcgg caaaaccccg gaagcgtgca tcagcacgcg accggagcga
     6301 gcgaggaagc ctgcaaaaag gcaggcttca aagatgccgt tgagttttaa accggcggca
     6361 gatcgaacag taacacttcg ctgtcgctat ccgcatggat ggagattgcc tgctcatccc
     6421 agattgccag accatcgctg gtcgaggctt tcacgccgtt aatggtgaca ttgcctttca
     6481 ccacctggat ccagacgcgg cgttcagcgg caatctgatg caccgactgc tcatctttca
     6541 gcaacgccca acgatacagt tccatatcct gatgcacttt cagcgaacca tctcgcgcat
     6601 ccggcgagag caccagctgt ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg
     6661 gcgtaatgcc attttcttca ggcatgatcc agatctgata cagatgcaga cgctcggtgc
     6721 tgcttgggtt gtactctgag tgacgaatac ccgtacccgc actcataatc tggaactcac
     6781 ccgccggaac ctgctcttta ttgcccatgc tgtcctgatg ctcaacagta ccttccagca
     6841 cgtaggtcaa aatttccata tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa
     6901 tcacgtcgtc gttaatcacg cgcagcgcgg aaaagcccat aaagttcgga tcgtaatagt
     6961 tggcaaaaga gaaagtatgc caggagtcca gccagccatg atttgcatga ccacgttcat
     7021 ttgctttgcg taagtagatc atttgtattc gccccatgaa tgatttcgat gggcttagtg
     7081 tggacccgat ctgcctggga tgatagaggg tgaaaattga cccctctgtt caaaaaaatt
     7141 gaactattcg aggggccatc aaacttactt agcgagagtt actgtggagg gagaggcttg
     7201 ctcaaatccg cgttcaagga tttccagatt cgtaagaact tcagattcct tgacgtaatt
     7261 tggcgcaccg tgggtgatgg tttgatacaa cgcatcataa acgcgcccgt aatcgcccat
     7321 ctccggcttc atctcttctc tgaccgtcac gccctcgtca ttgacatact ccagcacacc
     7381 gaccgaatca tccgctgcga atcccggttc gcccggcata atattagcct tcaggctggt
     7441 ttcctgctgg tcgataccgt atttaataaa cgaacctttc ttaccgtgaa cgataaattt
     7501 cggataatcg attttcacca gatggctggt tttgacgatg gctttcaggt cgccataaaa
     7561 cagttgcgct tcaaaggtgt cgtcaggatt ggctttatta cgcaggctgc ggatgtcata
     7621 agcgacgtga tccgggcgac cgaacagaga aataatctgg tccatcgtat gcacaccaag
     7681 gccataaaac gcgccatcct gcggcagccc aggtttggtc tctgccaccg ggcggtaata
     7741 gtcaaaatgg ctttccactt caacaatctc tcccaacttg ccactttcaa tcgctttttt
     7801 cgctgtcagg aagcaggagt caaagcgacg attctgatac ggcgtgacgg tcagcccttt
     7861 gcttttcgcc aacgcaaaca gctccttcgc ctgggcaagc gtcggggtga acggtttttc
     7921 gaccagcaca tttttcccgg cttccagcgc gcgtttcgcg tactcgaaat ggctgtccgc
     7981 gtgggtgcag acaacaacca gcttaacatc gggatcgttt agtacttcgt cgagatcgct
     8041 ggtgaaatgg atatgggaat aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa
     8101 aatatgcgcg acatgccagc tatccttgcg gttaagtaca tacggcagat ggtaacgggt
     8161 ggtgcttttg ccgaagccaa taaaggcgca gttgatgacc atgatttcgt cctttttaag
     8221 gtggttattt acaccttagc gcaaagcgga cgtggttcct actggagtgc gcataacgaa
     8281 cacaagcact cccgtggata aattgagaac gaaagatcaa aaaaaaagcc agcacccggc
     8341 tggctaagta atactggaag caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg
     8401 tcttcctgat cgcgagacaa taataatcat tctcattcgc acttgtccaa cactttttgc
     8461 aaaaaaatgc atttgactcg catttgaaag tcaatgatgt tgaaagggac atttacccca
     8521 aagaggacaa aggaatgagt gagatagtaa tacgccacgc agaaacacgg gattacgagg
     8581 ccatcaggca gattcacgcc cagccggagg tgtattgcaa cacactacag gtgcctcatc
     8641 cttccgatca tatgtggcag gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg
     8701 cctgtattga tggagacgtc gtgggccatc tcaccattga cgtgcaacag cgcccacgcc
     8761 gcagtcatgt tgccgatttt ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg
     8821 ccagcgccct gatgcgagag atgattgaaa tgtgcgacaa ctggttgcgg gtagatcgca
     8881 ttgaactaac cgtgtttgtc gataacgccc cggcaattaa ggtctataaa aaatacggct
     8941 ttgaaattga agggactggt aagaagtacg cattgcgtaa tggtgaatat gtcgatgcat
     9001 attatatggc gcgggtgaag taagatagtg ccctttttct gagatggaaa aagggtgtca
     9061 ttcaaaatcg acataccttc ctttaaggta tttatttgcc caatacataa attgagattt
     9121 aactcataat tacttcctaa agtgtaatat tttttttaat atatacgcct acaatttcct
     9181 ggagtaaata aataacaatt aacaagcata atattgccat tgataaaata gcatgccata
     9241 aaaggacttt tcagggatga gtaatattgt ttacctgaca gtaacgggag aacaacaagg
     9301 aagcatctcc gcaggttgtg ggacttctga gtctacaggt aatcgttggc agagcgggca
     9361 tgaggatgaa atatttacat tctcactctt aaataatatt aataatacgg ggcttggttc
     9421 acagttccat ggtataacat tttgtaaatt aattgataaa agcactccat tatttattaa
     9481 ttccattaac aataatgaac aattatttat gggatttgac ttctatcgaa taaatagatt
     9541 tggtagatgg gaaaagtatt attatataca actaagaggc gcttttttat cggctattca
     9601 tcaccagatc attgaaaacc aactggatac agaaacaata actattagtt atgaatttat
     9661 cctctgtcaa catcttatcg caaataccga gttcagctat ttggcactcc ctgaaaatta
     9721 taaccgtttg tttttaccaa attcaaaaaa ccaaacaaat aatcgtttca aaacgttaaa
     9781 cagcaaagct attggcaggc tacttgctgc tggtggcgta tacaatggga acattgaagg
     9841 attcagagat actgcggaaa aactgggtgg agatgcaata aaaggctatg atcaaatact
     9901 aaatgaaaaa acagcgggca tagcgatagc aacagcatct attcttttaa caaagcgttc
     9961 taatgttgat acatatacag aaataaatag ttacttaggc aaacttagag gtcaacaaaa
    10021 acttcttgat ggtatagaca taatagaaat aatatacatt aagagacctt caaaagactt
    10081 agctaactta cgaaaggagt ttaataaaac tgtaagaaaa aattttctta tcaaacttgc
    10141 aaaaacctcc gaagcatctg gaagattcaa cgccgaagac cttttaagaa tgagaaaggg
    10201 caatgttcct ctaaattata atgttcacca taaactatct ctagatgatg gtggtactaa
    10261 tgatttcgaa aatttagtat taatcgaaaa cgaaccatat cataaagttt ttactaacat
    10321 gcaatcacga atagctaagg gaatattagt aggtgaaagc aaaatcactc cctgggccat
    10381 tctatctggc tcaatttatc ctcccatgaa aaatattatg gaccacacaa aatgatgact
    10441 aaaacccaaa taaataaatt aataaaaatg atgaatgatt tagactatcc atttgaagca
    10501 ccgctcaagg aatcatttat tgaaagtata atccaaatag aatttaattc taattcaact
    10561 aattgcctgg agaagttatg taatgaagtt agtattcttt ttaagaatca acctgattat
    10621 cttacttttt taagagcaat ggatggattc gaagttaatg gattacgatt atttagcctc
    10681 tcgattccag aaccttcagt taaaaacctt tttgccgtaa atgaatttta tagaaataat
    10741 gatgatttca taaaccctga tctacaagaa cggttagtga tcggggatga tagcatttca
    10801 atatttactt atgacattaa aagtaacttt tttgaaatta gagataatat tggcacagaa
    10861 aatatattca gttcgtttag tgatttctct tcattcttaa acgaaatcat ggacagttgt
    10921 tcataataat caaaaggaaa caaaaatcat tgtaaaccat gccatctttt tagagattac
    10981 cagcagaaaa ttccttgttc gaaatttagc agtgcaagct atggaaaaac ttacaacgga
    11041 actacactcc ttaagtgaga tggaccgaag acatgtagtc tctattttag ccgaaatagc
    11101 taatggctat gatgatttta atgacatgtt gatttattta gaattttacc cgaatcataa
    11161 gattctgata tcttaaataa gaaaatcccg ttcgacgttc tgaaaaagaa atcataaaat
    11221 attagcagat acttatttcg tgactatatc ttatccgatg aacttttttc aaaaccagca
    11281 tttaataata tctttgacga agatgaacgc gtctttgctc aatgtatcac acaggaataa
    11341 taatataaca ctcaacaata atatttatta taattatgat tacttatcac gacgcattcg
    11401 cgaaagcgaa ccattacctt gatgatgcag atctcccggt cgtcattact ctacatggac
    11461 gctttagcca gggctggtat ttctgtttcg aagcacgaga atttctcgaa actggagatg
    11521 aggccgcgcg cttagctggt aacgcacctt ttattattga taaagacagt ggtgaaattc
    11581 attctctggg aacggcaaag ccgctggaag aatatctaca ggattacgaa ataaaaaagg
    11641 ctaccttcgg cttgccctga caaaatagcc ctcttcccac gaagagggcc gctaacctta
    11701 gtaccccgcc gttaaatcat ccaccgagcg cgggtcggat gcgccgtaca actcaccgtc
    11761 cggcccaacc ataatgcttt gtgtactgcc catcgcctct ttcagcgcca ctttctgacc
    11821 ttttgcttcc agcagcttga gcgtatccgg gctaaaccct ttttcgacac gcagctcgtc
    11881 cggcaaccac tgatggtgga aacgcggcgc attggtcgct tcggcgacgt tcatgccata
    11941 atcgatgcta ttcaccacca tttgcagcac tgtagtgatg atccggctac cgcctgggct
    12001 accggtaacc agccaggttt taccgtcttt caccacaatg gtcggcgaca tcgacgacag
    12061 cgggcgtttg ttcggcccga cggcgttggc atcaccgccc accagcccgt aaacgttcgg
    12121 tacgcccggt ttggcggaga aatcatccat ctggttatta agcagaatac cgctctcgcc
    12181 cgcgacaata cccgtaccga aggtggtgtt cagcgtatag gtcaccgcca ccgcgttacc
    12241 gtctttatcc actacagagt aatgggtagt ttgattactc tcataaggcg caagcttgcc
    12301 agggcgaatc tcactggacg gcttcgcttt gttgatatca atttgatcgg caatggattt
    12361 ggcataggct ttattggtca gtgcctgcca cgggactttg acaaaatccg ggtcgccaag
    12421 atattccgag cggtcggcgt aggcgtattt ctccgcttct gccatgattt gcatcgcatc
    12481 ggcgctgcca aagccgtatt tcttcatatc gaagttttcc agaatattga ggatttgtac
    12541 gatatggatc ccgccggagg atggcggtgg catggagtaa acctgatacc cgcgataatc
    12601 gccgcttatc ggagtgcgtt cgaccgcttt ataggccgct aaatcttctt tagtgatcaa
    12661 gccaccgttt ttctgcatct cctgggcgat ctgttccgca atcgtgcctt tatagaattc
    12721 gtccgggccg ttttcagcaa tcatctccag gctctttgcc aggttcgcct gcaccagcgt
    12781 gtcgcccttt ttcagcggtt cgccctcttt ccagaagata gctttgctgt tttcgtgatt
    12841 cggcaacact tcgctaccgt aggttttgag atcgtctgcc aaagcatcgt taacgataaa
    12901 accatcgcgt gccagtttga acgcgggctg caccactttg ttcaacggca tagtgccgta
    12961 cttatccaac gccagcgaga aaccagcgac cgtacctggt gtgccggaag ccagatgcga
    13021 agtgagtgat tttttgctgt ccgggttgcc ctggtcatca aggaacatat cgcgggtcgc
    13081 tttagcgggt gccatttcgc ggaaatcgat agcggtggta ttgccatttt tcgagcggat
    13141 cagcataaaa ccaccaccgc ccagattccc tgcctgcgga tgcgttaccg ccagcgcgta
    13201 gcccaccgcc acggcggcat caacggcatt cccgccctcc ttgagaatat ccacccccac
    13261 ctgagtggca gtggcgtcca cagacgctac cattccctgt ttcgcgcgta ccgggtggaa
    13321 gacatcttcc tccacaccat acgagacggg cggcgcagga ggcgcggcgg cggcgctaaa
    13381 acaacttcct gagagcagag cagcaatggc cacccggcgt aaaaacgtcg gttttatcat
    13441 cgttattctc cagagattaa ggggcaaccc cagctaagcc tggtatataa ctctgaatta
    13501 atcatcgttt tgtcgggaag cgagtaaact taaaggatat cttcagagga ggatacgcga
    13561 tgaaacgact tctgattctt acggcactcc tgccgtttgt cggctttgca cagcccatta
    13621 atactctgaa caaccctaac cagccggggt atcagatacc cagccagcag cggatgcaaa
    13681 cccagatgca gactcagcaa atccagcaaa aagggatgct gaatcagcaa ctgaaaacgc
    13741 aaactcagtt gcaacagcag catttagaaa accagataaa caataattct cagcgggtgt
    13801 tgcagtcaca gccgggggag cgaaatcccg cccggcagca aatgctgccc aacaccaacg
    13861 gcgggatgtt aaacagcaac cgtaatccgg atagttcgtt gaatcagcag catatgctgc
    13921 cggagaggag aaacggcgac atgctgaatc agcccagcac gccgcagcct gacattccgt
    13981 tgaaaactat tgggccgtaa agttcggacc aatcacgtca atcgcatcgg tacagatgca
    14041 atccacgccc cagcgcagca actctgccgc gcgctggggt ttattgacgg tataaaccag
    14101 aatccgcagt ccggcgtctt tcaactgcat cactcgcact ttatcgagta acttatgatt
    14161 gagatgaata gagacgcagc ccagccgcgc ggtcagttcg cgccagtcgt cgcgccactc
    14221 atcaagcaat aaaccacgcg gcagttccgg tgccgccagc tgtgcagctt ctaaagcatc
    14281 aatctcaaac gatgacagca gcggcggcgt cataccggcc cacagttggc gtgccgccag
    14341 cgccaccatt ttgcccgtta atggtccagt gccagtggtg ggtttgattt cgatattcgc
    14401 catcatcccg tgttcgcgac agcgttccgc cacctgcgaa agcaacggca gcgactcacc
    14461 tttaaacgct ttgctgtacc aactgcccgc atccacgcgc agtaaatcct gccagttcag
    14521 ttcacccgcg acgccccagc cgttgctggt gcgttcgaga ttgtcgtcat ggagcaggaa
    14581 gatctcgcca tctttcgata acttcgcgtc aaattcgatc atcttatgac cgtattttgc
    14641 cccgacgtcg attgccgcca gggtgttttc cggggccagc ttaccgccgc cacgatgagc
    14701 gacgatgcgg ggataaggcc agttactcat actcgttgtc ctgtttcacc atcaaaaaga
    14761 tgcagctgat tttccgccag atgcagccac agcgtgctgc ctgccgtcgg gcgctcctga
    14821 tgcgccagtc gcaccaccag cttctgctcg ccccagcgtc cgtgcgccag gttatctgcg
    14881 ccgaggatct ccagcgtgtc catcaccagc ggtacgccgc cttctgcctg cgagcttagc
    14941 gcaatatgtt ccgggcgaat gccgagagtc attttacgcc cggcatactg acggtagcca
    15001 ccgtttagcg gcagctcaat accgccgtcc agttcgaaat gcgtgccttc gttattcacg
    15061 cggcctgtca gcaggttcat cgccggactg ccgataaaac tcgctacaaa caggctggcg
    15121 ggcttttcgt agacttcaac tggtgtgcca atctgttcgg caacgccgcc gttcatcacc
    15181 attactcgct gggcgagcgt catcgcttca acctgatcgt gagtaacgta gagtgaagtc
    15241 gttttcaggc gacggtgcag ctgttgcagc tcaaggcgca tctgcacgcg cagcttggca
    15301 tcgaggttag agagcggctc atcaaacagg aacaccgccg gatcgcgcac aatcgcgcga
    15361 cccatcgcca cacgctgacg ctgaccgccg gatagctcgc gcgggcggcg cttaagcagg
    15421 ccatccagct ccagaatgcg cgccgcttct ttaacgcgct cggctatttg ctgcttgccc
    15481 atgccgcgaa ttttcagccc ccacgccatg ttttcttcga cgctcatatg cggataaagc
    15541 gcgtagttct ggaacaccat cgcaatcccg cgatctttcg gctccatttc ggtcacgcgc
    15601 tggtcgttga tccagatatc gccttcggtc acccgctcca gcccggcaac catgcgcagc
    15661 agcgtcgatt tcccgcagcc agacggcccg accatcacga taaattcgcc atctgccaca
    15721 tcaagggtca gcggtttaat gacctgggtt ttgccatccc agcttttggt tactgcctgt
    15781 aatttcagtc ctgccatctt atttctcact atcgaccagg ccacgcacga aggcacgctg
    15841 catcactaaa acaatcacta ctggtgggat cagggttaac aacatcgccg ccatcactga
    15901 gttccattcc gtggtgcctt cgccagtggc gatcatccct ttgatccctg ccacggtggt
    15961 gccgagatca acgtcggtaa taatcaacaa cggccacaaa tactgattcc agccgtagat
    16021 aaaggtgatc acaaacagcg ccgccagatt agttttggag agcggaaaaa cgatgtcgca
    16081 aaagaagcgc attggcgatg cgccgtcgat ccgcgcggct tccaccagct catccggcag
    16141 cgtcataaag aactggcgga acaggaaagt agcggtcgcc gaggccatca gcggcagcgt
    16201 taaaccggcg tagctgtcga gcatcttcag gttggcgatg acttccaccg tcgggaagat
    16261 acgtacttca accggcagca tcagggtgat aaaaatcatc cagaagaaga ggttacgtag
    16321 cggaaaacga aaccagacaa tggcaaatgc cgagagcatc gagacggtaa ttttgccgag
    16381 cgtaatgctg aacgccatca caaagctgtt aagcaacatc cgccagaacg gcgcgctatt
    16441 cgtacctacc ccgttaaccc agatgttgtg gatattttcc agcagatatg tgccggggat
    16501 gagcgtcatc ggcgcggcat agacggcctg tttatccagc gtcgccgcga caaacgccac
    16561 gtacagcggg aagaggatca ccgcgatccc gaggatcagc atggtatggc tgaatatcgt
    16621 cagccacgga cggttctcaa tcattggtaa cgcaccttgc tttcaacata gcggaactgc
    16681 accaccgtca gcacgatgac gaggaacatc aacaccaccg actgtgcggc agacgaagcc
    16741 agatccagtc cggtaaaacc ttcgcggtag atcttataaa tcagcgtcgt ggtggcctga
    16801 accggcccgc cggacgtggc ggcgtcgatc accgggaagg tgtcgaagaa ggcatacacc
    16861 aggttcacta ccagcaggaa gaaactcacc ggagcgataa gcggcagcgc aatcttaaag
    16921 aagcggcgaa ttgggcctgc accgtcgatg gctgcagctt caatcaatga acggggaatg
    16981 gagtgcagcg cggcatagaa gaacagaaag ttgtagctga tttgcttcca caccgaggca
    17041 aacaccacca gaaacattgc ctgaccgctg ttctgcgcat ggttccagtc ataaccgaac
    17101 tcagcgagaa aatgggtgat cagcccacga ccggggttaa acaggaagat ccacaatacg
    17161 gcggcaacgg cgggagccac ggcatacggc agcagcatca aggtttgata gaaacggctg
    17221 ccgcgcacga tgtactccac caacgccgcg aagaacagcg acaccagcaa accgctgacg
    17281 gtgacaaagg tgctgaattt tatcgtcgtc cagaaggagt cgagatagta gctgtcatga
    17341 aacagcgcga cgaagttatc caggccgaca aactggctgg agaaaccaaa cggatcgacg
    17401 ctttgtagcg agtaccacaa cgcttcgccc gcaggccaga taaaaaagat aacggtgatg
    17461 atgagctgcg gcgcgaccag cagataaggc agccagcgcg agcggaacac cggacgggat
    17521 gatgacatta cggttaattc ctgaactgtg ccggatgcgc ttcgcttatc cggcctacac
    17581 gcgatgccag gtcagataag cgaaacgcat ccgacattac actgattaag acttcgtcga
    17641 tttctcaaag cggcgcagca actgatttcc acgctcaacg gcggtatcca gtgcctgctg
    17701 tggtgtcttc ttaccggtcc acacgctctc cagctcttca tccacaatca cgcggatctg
    17761 cggcatgttg cccagacgca gccctttggt gaacggcaac ggcggcttat tcagcatctg
    17821 acgcgtcgcg gtatccgccc ctgggttttt ctcgtaaaag ccctgctcac gggtcaggtc
    17881 atacgctgct ttggtgattg gcagataacc ggttttctga tgccactcgg cagcgttttc
    17941 tggcttcgcg aggaagtcga ggaacttcgc cacgccggta tacgtttctt tatttttgcc
    18001 ctgcatcacc cacaggctgg ccccgccgat aatggcgttt tgcggtgcat ctttcgcatc
    18061 ggcgtcgtaa ggcatcatgc ctacgccata gttaaatttg gcgtactcac gaatattagc
    18121 gagagaaccg gaagaggcgg tggtcatcgc gcaatcaccg ttatagaact tctcggtgga
    18181 ttcatcctta cgaccgacgt agctgaagtc gcccttcttg ttcatctcct cgagcatggc
    18241 gatgtgtttc acctgctccg gcttgttgaa ctccagcacc gcgtccgtgc cgtcaaagcc
    18301 gttgtttttg ctggcaaacg gcagaccgtt ccaggcgcta aagttttcca gttggatcca
    18361 gccctgccag ccgctggcgt agccgcactt cataccggag gctttcagtt tcgcggcata
    18421 gtccgccaga tcctgccagg ttttcggcgg ttgttccggg tctaatcctg ctttcttgaa
    18481 ggcgtctttg ttgtaataga gaacgggggt cgagctgttg aatggctggg agagtaagtg
    18541 gcccgttttg ctgtcggagt agtaaccgga aaccgtcggc acaaactgcg actcatcgaa
    18601 ttgaatcccc gcctctttaa acacgtcata taccggttta atggctttcg atgccatcat
    18661 ggtggcggtg ccaacttcat aaacctgcaa aatagccgga gcgttgccgg tacgaaatgc
    18721 ggcaatcccc gcgcttaaat tctgttcgta gttgccttta taggtcggta caattttgta
    18781 atccggattt tcggcgttaa aacgttgggc cagagaatcc acctctttac ccagttcccc
    18841 ttccatagaa tgccagaacg gaatggtcgt cactgcctgt gcattcccca ttaacgccag
    18901 tccgagcgcc agtgctgaag ctgtataatg taacggtttc atcgtttatc tctcttgttg
    18961 taccgaatgc gcgaattcac gcgttttatg ctcgcggggt aacatgacat gctcgaatta
    19021 cagaaaaata acttttttgt tacatttgta agatagtaag gtgtcagaaa gatgacaagg
    19081 cggtgacggc gtgggtgagg gaaaatggga gatggggcac ggataagcgg aaaatagaag
    19141 gtctgaatca aactctacag attactcatc gtttcatgcc ggatgcggcg taaacgcctt
    19201 atccggccta caagatcgtg caaattcaat atattgcaac tcacccagta ggcctgataa
    19261 gcgcagcgca tcaggcaatt ttacatttgt cacctgtctc aaaggagtct tttgactcct
    19321 tatcaatcaa cgtgttatta cccgcctaaa tacgcacttc tcactgcttc gttcgccagc
    19381 agcgcgtcgc cggtatcgga gagcaccaca tggccgtttt ccagcacgta gccgcgatcc
    19441 gccagcttta gcgcctggtt ggcgttctgc tcgacgagga agatagtcat cccctgctcg
    19501 cgcagctgct cgatggtgtc gaaaatttgc tggatgataa tcggcgcaag accgagcgat
    19561 ggctcatcaa gtagcagcaa acgcgggttg ctcatcagcg cgcgaccaat cgccagcatt
    19621 tgttgctcac cgccggacat ggtgcctgcc cgctgaacac ggcgctcatg cagacgtgga
    19681 aacagttcat acacccactt tatgcgctcc tggaactggt cgcgttcggc aaaaaagccg
    19741 cccatcgcca ggttctcttc caccgtcatc cgcgagaaga cgcgacgccc ttccgggaca
    19801 atcgctaccg cttcgcgcat gattttcgcc gtctgccagt cggtaatgtc tttatcatca
    19861 aacacaattc gcccgctggt agcgcgcgga tcgccgcata acgtgccgag caaggtggtt
    19921 ttccccgccc cgttcgcgcc aatgagggtg acaatctcgc cctgattgat atgcaggctc
    19981 acctcatgca gcgcctggat tttgccgtag tgggcgctga ctttgtcaaa ggacaacatg
    20041 actttttcca tcttatgcct cacctaaata ggcacggatc acgtccgggt tattacggat
    20101 ttgctccggc gtaccgtttg ccagtggcgt cccctgattg accacataaa ttcggtctga
    20161 aatccccatc accagcttca tatcgtgttc aatcaacaag atagtggtgt tgtgatgatt
    20221 gcgcagttcg gcaatcagct catccagctc tttggtttct tttgggttaa gacctgccgc
    20281 cggttcgtcg agcattaaaa tctccggctg cgtcaccatg cagcgggcaa tctccagacg
    20341 gcgttggtca ccataggcca ggttactcgc ctgacggttg gcgtgttcca gcaaaccaat
    20401 gcgctcaagc caggtggcgg cgcggtcgag cgcttcgctc tgggcgcgac ggaaagaagg
    20461 cgttttcaac aggccagaga acagcccggt tttcagttgc tgatgctgcg ccaccagcag
    20521 gttttcaatt accgtcattt cacggaacag acgcacatgc tggaaggtgc gcaccacgcc
    20581 catgcgggca atttgctgcc ccggtaaacc ttcaagatgc tgatcgcgca gtaaaatggt
    20641 gccgccggtg ggtttgtaga aaccggtcag gcagttaaaa accgtggttt ttccggcacc
    20701 gttagggccg attaacgaga cgatctcctg cgggtacagt tcaagattga cgttgttcac
    20761 cgccagcagg ccgccgaagc gcatcatcag gccgttcaca gataataatg gctgactcat
    20821 gcctgctctc ctttcgctgc gccgtttttc agcttcagtt gcgggcgcgt catgggcagc
    20881 aagccctgcg gacgccagat catcatcagc accatcaaac caccgagcat taacatgctg
    20941 tattcgttga aatcacgcat caactcgcgc gacaccacca gcaaaattgc cgccagaatc
    21001 accgcaaact gcgatcccat accgccgagc accactatcg ccagtacaaa cgccgattca
    21061 gcaaaggtga aggattccgg gctgacaaag ccctggcgcg cagcaaacag cgttccggca
    21121 aaaccggcaa acgcggcact gatggtaaaa gcggtcagct tgatacgacg cgggcttaag
    21181 cccagcgaac ggcaggcgat ctcatcttca cgcaacgctt cccacgcacg ccccagcggc
    21241 atccgcagca ggcggttaat gacaaacagg cttagcacca ccagcagcaa cgccaccaga
    21301 tacaggaaga tgacgcgatc ggagggatcg tatttcaggc caaagaaatt actgaacgtg
    21361 tcccaaccgc cttcacgggc ggtacggctg aactcaaggc cgaagaaagt cggtttcggg
    21421 atctgactga ttccgttcgg gccgccggta atttcggtgt tattgagcag caatatgcgc
    21481 acaatttcgc cgaagccgag ggtaacgatc gccagataat caccacgcag acgcagcacc
    21541 ggaaagccga gcaggaagcc cgccgccgct gccattaacc cggcaatcgg cagacaggtc
    21601 cagaagccca agccgtaata gtgattgagc agcgcaaaag tgtaagcgcc gatggcgtaa
    21661 aaaccgccgt accccagcac cagcaggccg gaaagcccta ccaccacgtt cagcccgagg
    21721 ccgaggataa tgtagatcat ggtcagggtg gcgatatcca ccgtcccgcg tgaaaccata
    21781 aacggccacg ccaccgcaag caccaacagc gccacgagga acagtttctg cttcaccgtg
    21841 gagccatcaa tcgcgggcag aataaacttc ggcccggaaa cgcttttcaa ccctttctgg
    21901 aaagcaggtc gcaaaatctg gaagaaaaag accaccgccg tgccgataaa cacccactgc
    21961 caacggacat ccgaagccgt gtcgaccacc agtttggtgc cgtccagctc cagctgtacg
    22021 cccataaaga cgccagcgag gataaagaac atcgcggcag agagcagcgc cattgcaata
    22081 tgcatcggtt tcatactttc tctacctccg ggcgccccag aataccggtc ggcatcacca
    22141 gcagtaccag gatcagcagg gcaaatgaga ccacatcttt atattccgta ctcagatagg
    22201 cagaagagag cgcctccgca atccccagga tcagcccgcc aatcatcgcc cccggaatgc
    22261 tgccaatccc gccgagcacc gccgcggtaa aggctttcat cccggccata aagccgatgt
    22321 aggggttaat cacaccgtag aactgaccga gcagcacacc cgccaccgcc gccatcgccg
    22381 cgccaatcac aaaggtcagc gcaatcaccc ggtcggtgtt gatgccaagc aggctcgcca
    22441 ttttcagatc ttccgcgcag gcacgacagg cgcgccccat acgggaatag cgaatgaaaa
    22501 tcgtcagcgc cagcatggcg aggaaggtaa caatccaaat caccgcctgc atggtagtaa
    22561 tagaggcaga gaagttttcg ctatggccca ccacccactg cccgttaaac agactgggca
    22621 gcgccacgtc acgcgaacct tcggtcaggc taacgtagtt ttgcaggaag atggacatgc
    22681 cgattgccga gatgagcgca atcaggcgct tagagttacg caccgggcga taggctaccc
    22741 gttcgatact ccagccgtag gcgctggcaa tgacgattgc gccgacgaat cccgccgcca
    22801 ccagcagcca gccggtatca atgcccatca tcatcagcgc ggcgatgatc ataaatgaga
    22861 cgtagctgcc aatcatataa acctcgccgt gggcgaagtt gatcatgccg ataatgccgt
    22921 aaaccatggt gtagccgatg gctatcagcg cgtaggtact gcccagcgtg acgccgttaa
    22981 acatctgctg caagaaatac aaaaactgct cagacataag gtaacctttc taaacccgcc
    23041 cgcattttac gggcggtggg atgatcactt ggctgccgtg gatgaaccgt cggcgtgcca
    23101 ctggaagaca ccaaaatcaa atcccttaag atcgcctttt tcatcccagt tcagcggccc
    23161 aatcacggtg tttgcaccgt tagcttttaa atctttcacc agcgccagcg gctcatcgct
    23221 gccagtacgc tcaagggcag tcgccagaga ttgcaccgcc gcgtaggtga tccagacata
    23281 aggcccggac ggatctttct tgtctgcttt cagcgcatca acgatgccct ggtttgccgg
    23341 atcctggtca tagcgttttg gcatagtgac caacatgcct tcggcagcat caccggcaat
    23401 attcgacaat gatgcgttac ccacaccttc cggccccata aactgagttt tcaggccaac
    23461 ggaacgggcc tggcgcagca tctgccccat ttccgggtag taaccgccgt agtaaacgaa
    23521 gtcgatgttt tcttttttca ggcgggcgat cagcgcggag aaatctttct ccccggcggt
    23581 aataccgtcg aagaagacga cgttggcgtt agccgctttt agcccgtcct gcaccgaacg
    23641 cgccagccct tcgccatact gttgtttgtc gtgaatgatg gcgatgcgct ggggcttcac
    23701 cgtctcaaga atgtattttg ccgccgtagg cccctgggaa gagtccagcc cggcagtacg
    23761 cataatgtgt tgataaccgc gttgggtcag ctccgggttg gtcgctcccg gcgagatcat
    23821 cagaatacct tcgtcttcgt agatatctga cgcaggttgg gtagaagaag aacacagatg
    23881 accaataacg tatttaatgc cgtcgttaac gattttgttg gcgaccgcaa cggcttgttt
    23941 cgggtcgcag gcgtcgtcat attccacgcc caccagctta tcgcctttaa ttcccccttt
    24001 ggcattaatg tctttaatcg cctgacgcgc gccgttaaat tccatatcgc cccactgggc
    24061 aatcgggccg gacatcgcgc cgacaacggc aactttaata tcgtcagcca tagcggtgtg
    24121 tgaaattgcc agtgcaatca tccctgcgat gatagttttc gcattccgtt tcatagtcaa
    24181 aaatccccat tcgtgatgtt gtgttgcttt gtttttatgt gttaacaaat cagactgttc
    24241 tttttttata ctgcactgtt tttgcctgtc tgattaaggg tttagcgcag tattttgtga
    24301 taatagtgat taaaatccct atttttcagt cgattaagaa cagataatat tctgaattta
    24361 ttgatagata aacagaaaaa agtgcctttg tcagcataaa ataacggcac aaagggcgga
    24421 ataattcact accattcagg ggattatgct gggtattttt cattctctaa tgttttaact
    24481 ttgtaattat tgctgttaaa aaattaatca cctgccaaaa gaaataaaaa agagaaagcc
    24541 tccgattaaa ttatttcgct acactggttc cacttttgtg atttacacgg gttacccatg
    24601 aagctgacca tcattcgatt agaaaacttt agcgaccagg accggattga cctgcaaaaa
    24661 atctggccgg agtattcccc ttcctcttta caggttgacg ataaccaccg tatctacgcc
    24721 gcgcgtttta acgagcgcct gctcgctgcc gtacgggtaa ccttaagcgg caccgaggga
    24781 gcactggatt ccctgcgtgt gcgggaagtc acccgacgtc gcggtgtggg gcaatatctg
    24841 ctggaagagg ttttgcgtaa caatcctggc gtttcatgct ggtggatggc ggatgcaggc
    24901 gtggaagatc gcggtgtgat gacggcgttt atgcaggcac tgggatttac ggcgcaacag
    24961 ggcggctggg agaagcgtta atcgctctgt gtgaaaagca gttcgatgga tttggttggg
    25021 aaaaataacg cattttcgtt ttctccgacc aacgggataa agcccagcga ttgataaaac
    25081 gtatgggctt ttttattcag cgcctcgaca aaaagaccgt gaatacctac cgctaaagag
    25141 gctgaccaga cgactttcat ggcatgagca accagtgttg ctccccatcc ctgcccctgt
    25201 aatgaacgat caattgccag acgcccaaga gtaacgctgg gaatattttt gtagggaatt
    25261 tttttctgtt tcgatttcga gggcaatgcg gctcgttcaa aacaactgcc gcataatgtg
    25321 taatatccca gcacctgacg ttctggagtg ttgcgacaaa ggatatacgc tcgcagaatt
    25381 ttgttgcgat gttgacgaac gagatgtgtc gtcagaaaga gatttaacgc ttcctcgccg
    25441 cagtcgaatc gctgtagatc ataatctgca tcatcggtca gaatctctat cgtcagatca
    25501 tccataaatt attacattcc ctgaagacgt tttgccgcac gttttagctt ttcaccaggt
    25561 gacggtggat tactcagcgc atccatcacc cgcgtccagg attcctcatt gaggatcacc
    25621 cgccgatgct gttcaatcac ttccgcagcc cgctgcgaag cgctgttcaa cataaattgg
    25681 ctgacggact gattagatat cgctgccgct tcctcaatca tacttttgtc atcgtcagtt
    25741 aaacgcagat cgatacgctg ctttttaaca gcagacatgt gaactctcct gctccgtata
    25801 atatgtacgg tatgtcaccg tacattaagc atatcctgga ttgtttaata tttctacaac
    25861 catttcgaca aacacaaaaa aacccgatgg cagttcagcg catcgggcat tttcatttat
    25921 ctatctgtta cttcgcatcg gttgccgtgc cgttggcgtg ccagtcaaat acgccgaact
    25981 caaagccttt cagatcgcct ttctcatccc acgtcagcgg ccccatcacg gtatcaac
//
LOCUS       DAFATA010000060        25988 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFATA010000060 DAFATA010000000
VERSION     DAFATA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.061"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..2148
                     /gene="bisC"
                     /locus_tag="LGJ20_002960"
     CDS             <1..2148
                     /gene="bisC"
                     /locus_tag="LGJ20_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBK2386994.1"
                     /translation="RKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIREAYG
                     PASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVVGGSE
                     VYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICID
                     PMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYAVFAS
                     YLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGEQKHW
                     MIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVA
                     RIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVIS
                     ECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFA
                     ELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMP
                     ENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQ
                     GNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMV
                     RVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSR
                     LGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2117..2557)
                     /locus_tag="LGJ20_002961"
     CDS             complement(2117..2557)
                     /locus_tag="LGJ20_002961"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2386995.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2554..3117)
                     /gene="tag"
                     /locus_tag="LGJ20_002962"
     CDS             complement(2554..3117)
                     /gene="tag"
                     /locus_tag="LGJ20_002962"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK2386996.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3275..3973
                     /locus_tag="LGJ20_002963"
     CDS             3275..3973
                     /locus_tag="LGJ20_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2386997.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4202..5410
                     /locus_tag="LGJ20_002964"
     CDS             4202..5410
                     /locus_tag="LGJ20_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2386998.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTRLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5734..7425
                     /gene="eptB"
                     /locus_tag="LGJ20_002965"
     CDS             5734..7425
                     /gene="eptB"
                     /locus_tag="LGJ20_002965"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBK2386999.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7517..7593
                     /locus_tag="LGJ20_002966"
     tRNA            7517..7593
                     /locus_tag="LGJ20_002966"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7551..7553,aa:Pro,seq:cgg)
     gene            7628..7761
                     /locus_tag="LGJ20_002967"
     ncRNA           7628..7761
                     /ncRNA_class="other"
                     /locus_tag="LGJ20_002967"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8506..10113
                     /gene="dppA"
                     /locus_tag="LGJ20_002968"
     CDS             8506..10113
                     /gene="dppA"
                     /locus_tag="LGJ20_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBK2387000.1"
                     /translation="MRISLKNSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10421..11440
                     /gene="dppB"
                     /locus_tag="LGJ20_002969"
     CDS             10421..11440
                     /gene="dppB"
                     /locus_tag="LGJ20_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBK2387001.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11450..12352
                     /gene="dppC"
                     /locus_tag="LGJ20_002970"
     CDS             11450..12352
                     /gene="dppC"
                     /locus_tag="LGJ20_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBK2387002.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVNNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12363..13346
                     /gene="dppD"
                     /locus_tag="LGJ20_002971"
     CDS             12363..13346
                     /gene="dppD"
                     /locus_tag="LGJ20_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBK2387003.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13343..14347
                     /gene="dppF"
                     /locus_tag="LGJ20_002972"
     CDS             13343..14347
                     /gene="dppF"
                     /locus_tag="LGJ20_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBK2387004.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPALKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14377..15648)
                     /locus_tag="LGJ20_002973"
     CDS             complement(14377..15648)
                     /locus_tag="LGJ20_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBK2387005.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16124..16231
                     /locus_tag="LGJ20_002974"
     CDS             16124..16231
                     /locus_tag="LGJ20_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBK2387006.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16318..17997)
                     /gene="bcsG"
                     /locus_tag="LGJ20_002975"
     CDS             complement(16318..17997)
                     /gene="bcsG"
                     /locus_tag="LGJ20_002975"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBK2387007.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17994..18185)
                     /gene="bcsF"
                     /locus_tag="LGJ20_002976"
     CDS             complement(17994..18185)
                     /gene="bcsF"
                     /locus_tag="LGJ20_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBK2387008.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18182..19753)
                     /gene="bcsE"
                     /locus_tag="LGJ20_002977"
     CDS             complement(18182..19753)
                     /gene="bcsE"
                     /locus_tag="LGJ20_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBK2387009.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            20026..20214
                     /locus_tag="LGJ20_002978"
     CDS             20026..20214
                     /locus_tag="LGJ20_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBK2387010.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            20226..20978
                     /gene="bcsQ"
                     /locus_tag="LGJ20_002979"
                     /pseudo
     CDS             20226..20978
                     /gene="bcsQ"
                     /locus_tag="LGJ20_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            20975..23593
                     /gene="bcsA"
                     /locus_tag="LGJ20_002980"
     CDS             20975..23593
                     /gene="bcsA"
                     /locus_tag="LGJ20_002980"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBK2387011.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            23604..25943
                     /gene="bcsB"
                     /locus_tag="LGJ20_002981"
     CDS             23604..25943
                     /gene="bcsB"
                     /locus_tag="LGJ20_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBK2387012.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
BASE COUNT         6139 a         6553 c         7021 g         6275 t
ORIGIN      
        1 cgcaaaggct ttctcgcgtc accggaaaac ccgcaaggca ttcgtgggca ggatgaattt
       61 gttcgcgtga gttgggatga ggcgctggat cttattcacc aacaacataa acgcattcgt
      121 gaggcttatg gtccggcatc gatttttgct ggttcctacg gctggcgttc aaacggcgtg
      181 ctgcataagg cctcgacatt attacaacgc tatatggcgc tggcaggcgg ttatatcggg
      241 catctggggg attattcgac cggtgcggca caggcgatca tgccgtatgt cgtgggtggc
      301 agcgaagttt atcaacagca aaccagttgg ccgctggtgc tggaacatag cgatgtcgtg
      361 gtgttgtgga gtgctaaccc actcaatacg ctgaaaattg cgtggaatgc atctgatgag
      421 caggggcttt cttacttttc cgcactgcgt gacagcggga aaaagctgat ctgtattgat
      481 ccaatgcgat cggaaaccgt cgatttcttt ggcgataaaa tggagtgggt ggcaccgcac
      541 atgggcaccg atgttgcgct gatgctgggg atcgcccata cgctggtgga aaatggttgg
      601 cacgacgaag cgtttctgac gcgttgcacc acaggttatg ccgtcttcgc ctcttatttg
      661 ctgggcgaga gtgacggaat agcgaaaaac gccgaatggg cggcagagat ttgtggggtt
      721 aacgcagcga aaatccgcga gctggcggct cttttccacc aaaacacgac catgctgatg
      781 gctggctggg gaatgcagcg ccaacagttt ggtgagcaaa aacactggat gatcgtcacg
      841 ctggcggcaa tgttggggca aatcggcaca cccggcggcg gttttggtct ttcttatcat
      901 tttgccaatg gtggtaaccc cacgcggcgt tctgcggtgc tctcttccat gcagggcagc
      961 ttgccgggtg gctgcgatgc ggtggataaa atccctgttg cccgcattgt tgaagcactg
     1021 gaaaaccctg gtggcgcata tcaacacaac ggtatgaacc gacatttccc ggatattcgt
     1081 tttatctggt gggcgggcgg tgccaacttt actcatcatc aggataccaa tcgtctgatc
     1141 cgtgcctggc aaaaaccgga gctggtggtg atctctgaat gcttctggac ggcggcggcg
     1201 aaacacgcgg atatcgttct gcctgcgact acctcttttg agcgtaatga tctcaccatg
     1261 accggtgatt acagtaatca gcatctggtg ccgatgaagc aagtggtgcc gccacgctat
     1321 gaagcgcgta atgattttga tgtttttgcc gagttgagtg aacgttggga gaagggcggc
     1381 tatgcgcggt ttacggaagg aaaaagtcag ctgcaatggc tggaaacgtt ttataacgtt
     1441 gcccggcagc gcggggcaag ccagcaggtt gaattgccgc catttgctga gttctggcaa
     1501 gccaaccagt taattgagat gccggaaaac ccggacagcg agcggtttat tcgcttcgct
     1561 gatttttgcc gcgatccgct ggcgcatccg ttaaaaaccg ccagcggcaa gattgaaatc
     1621 ttctcacagc gtattgccga ttacggttac ccggattgcc ctgggcatcc aatgtggctg
     1681 gagccggacg aatggcaggg caatgccgaa ccagaacagt tgcaggtact ttctgcccat
     1741 ccggcgcacc gcctgcacag ccagctgaat tacagttctc tgcgcgaatt gtacgcggta
     1801 gcaaaccgtg agcctgtcac cattcatcct gacgatgctc aggcgcgcgg cataaccgaa
     1861 ggggatatgg tgcgggtgtg gaactcccgt gggcagatcc ttgccggagc ggtcattagc
     1921 gagggaatta aacctggcgt gatttgcatt cacgaagggg catggccgga tctggattta
     1981 accgctgacg gtatttgtaa aaacggcgca gtgaacgtgc tgaccaaaga tctccccagc
     2041 tcgcggctgg ggaatggctg tgcgggtaat acggcgctgg catggctgga aaaatacaac
     2101 ggtccggaac tgacacttac agcgtttgaa ccaccggcca gctcataatc caggtgggta
     2161 gttgggtttc atcctgccat gcgcaatcga caatgtgaaa accctgcgca cggtaaaaat
     2221 ctatcgccgg ttgatttttt tgataaacct ccagcatcag atggggatag cgctgctgca
     2281 catactgcat cagcgcctta ccaataccgc gtctgacggc cttcggtgcg acaaacattg
     2341 ctgccagaaa tcggccttcc ataatgctga caaaaccgag aagcttaccg tcctcttccc
     2401 agacccagtt ttgcgcgttg gcaagatagg catcccgcac cagcggaatg cagtcacgcc
     2461 agtaattcgc ttttataaag ggatgccccc aggttgtact ttccagccac agttcgagga
     2521 tcgcggggag ttctgaacgt tgcgcttccc gaatcatggt ttatttcccg gatagcaaca
     2581 gcagccaacc acatgatcat tcaccagccc acatgcctgc ataaaggagt aacagattgt
     2641 ggtgccgaca aacttaaaac cacgtttttt cagtgcctta gatagggcgt cggaggcgga
     2701 cgtagatgtg ggaatttcgc tcaacgttgt ggcttgtgtc acctgtggct gatgatttac
     2761 aaacgaccag acaaagtcgg caaacggttc gccgttctgt tccatttgca ggtacgcccg
     2821 cgcattacca ataattgcct gaattttccc tcgatggcgg ataatcccgg cgtcctgtac
     2881 cagtctttcg acatcctctt cctgcattgc tgcgaccttc accggatcga actgatgaaa
     2941 gcaggcgcga tagttttcgc gttttttgag gacggtgatc cacgataacc cagcctgctg
     3001 cccttcaagg cagatcattt cgaacagttt tttactgtca gtttcaggca cgccccattc
     3061 attatcatgg taggcaatat aaagcgggtc ctgactcacc cagccgcaac gttccatact
     3121 ttccctcgct atgtacccga tcgtaaaaat ttcagccgct tttcttgacg catcggtgaa
     3181 aaagtcaata tttaatacag ggctactatg cccgatatcc ttcatacaat gacaataata
     3241 ttgccgaatt cggctcttcc ctggagtcgt gttaatgatc ataaaaaaaa gcggtggtcg
     3301 ctggcagcta agcctgctgg cgagcgtggt aatcagtgcc ttttttctca acacagctta
     3361 cgcctggcaa caagaatata tcgttgatac gcaacccgga cattccacag agcgttacac
     3421 ctgggatagt gatcatcaac ctgattacaa cgatattttg tcgcaacgta ttcaaagtag
     3481 ccaaagggcg ctgggactgg aagtcaatct ggcggaagaa actcctgtgg atgtgaccag
     3541 cagtatgagt atgggctgga attttccttt atatgaacag gttacaaccg gcccggtcgc
     3601 ggcattacat tacgatggca caaccacctc gatgtataac gagtttggcg acagtactac
     3661 cacgctgacc gatccgttat ggcatgccag cgtgagtagc ttaggctggc gtgttgactc
     3721 ccggcttggc gatctccgac cctgggcgca aatcagctat aaccagcaat ttggcgagaa
     3781 tatctggaag gcgcaatcag gcctgagccg gatgacggcg acaaaccaga acggcaactg
     3841 gctggatgtc accgtaggcg ctgatatgtt gctcaatcaa aatattgccg cctatgccgc
     3901 gctaactcag gcagaaaata ccactaataa tagcgactat ctgtatacga tgggggttag
     3961 cgccagattt taacgtaaca gtcacaattg aaaccattga ataacaacag ttgtggcgat
     4021 agtgggtgct aacttaccaa ataataaatt tggtgaataa ttgtcgcgtc attcattcct
     4081 gaactaaggc atttcattcc gttctgatgg catttcatgc cgtttttccc caggcataaa
     4141 gtgcacttcg ttatggttgt cggcagagat ttttcctttt tattactgca ggaatactgc
     4201 catgacacct tcaaattatc agcgtacccg ctggctgaca ctcatcggta caatcattac
     4261 ccagtttgcg ctggggtcgg tttatacctg gagcctgttt aatggcgcgc tttccgccaa
     4321 gctggatgcg ccggtaagcc aggtcgcttt ctctttcggc ttgttaagtc tggggctggc
     4381 aatttcatct tctgttgcgg gcaaattaca ggaacgtttt ggcgttaaac gcgtcaccat
     4441 ggcttccggc attttgctgg gattaggctt cttcctgacc gcgcattctg acaacctgat
     4501 gatgctgtgg ttaagcgccg gtgtgctggt cggtctggcg gatggcgcgg gttacctgct
     4561 gacgctctct aactgcgtga agtggttccc ggagcgtaaa ggtctgatat ccgcgttcgc
     4621 tatcggttct tatggtctgg gtagcctggg tttcaaattt atcgacacgc ggctgctgga
     4681 aacggtgggt ctggaaaaaa cctttgtgat ttggggagcg attgcgctgg tgatgattgt
     4741 tttcggcgca acgttaatga aagacgcacc aaaacaggaa gtgaaaacca gcaatggtgt
     4801 ggtggagaaa gattacacgc tggcagagtc gatgcgtaaa ccgcagtact ggatgttagc
     4861 ggtaatgttc ctgactgcct gcatgagcgg cctgtacgtg attggggtag cgaaagatat
     4921 cgcccaaagt ctggcacacc ttgatgtggt ttccgcagct aatgcagtca ctgttatttc
     4981 catcgccaac ctttcaggtc gtctggttct ggggatactg tctgacaaaa tcgcccgtat
     5041 ccgtgttatc accattggtc aggtgatttc gctagtgggt atggcggccc tgctgtttgc
     5101 accactgaat gcagtgacgt tctttgcagc gattgcctgc gtggcattta actttggcgg
     5161 cactattacc gtctttccgt cactggtcag tgagttcttc ggcctcaata acctggcgaa
     5221 aaactacggt gtgatttacc tcggtttcgg tatcggtagc atttttggtt cgattatcgc
     5281 ctcactgttt ggcggtttct atgtgacctt ctacgtgatt ttcgccctgc tgattctgtc
     5341 attggcgctt tctacgacga ttcgtcagcc agagcagaaa atgttgcgtg aggcgcatgg
     5401 ctccctttaa tgctcagccg cgagctactg ggcttcagct tccgtgtgca tcaaacgcat
     5461 caacgaaatg tataaacaat gactcgtaaa atttgggcca gttaactggc cctttttact
     5521 gctttttgta acgctaaagt ctcttttcaa acttgcattt ttgtaaattt gtgcttcatg
     5581 cacactcttt ccccacactt tttcgctttg ctgtggtcta cttattcgcg cgtgtagatt
     5641 ttacttatct gactacctcc gcacttttcc cctgccgggc ctgaaaagcc gctaagcagg
     5701 gtgttatcac ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc gattacacag
     5761 cagaagctga gctttttgct tgcaatctat attggccttt ttatgaatgg cgcggttttt
     5821 tatcgccgct tcggcagcta tgcgcacgat tttaccgtct ggaaaggcgt ttctgctgtt
     5881 gttgaactgg ccgccaccgt actggtgacc ttctttttac tacgtcttct atcgctgttt
     5941 ggccgccgca gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc aggtgccagc
     6001 tattacatga ccttccttaa tgtggtcatt ggttatggca tcatcgcttc cgtcatgacc
     6061 accgatatcg acctgtcaaa agaagttgtt ggtctgaact ttattctctg gttaatcgcc
     6121 gttagtgcat tgcctcttat ccttatctgg aataaccgct gtcgctacac cttgctccga
     6181 caactgcgaa ccccagggca gcgtattcgc agcctggcgg tcgtcgtact ggcgggtatt
     6241 atggtttggg caccgattcg tttgctggat atccagcaga agaaagtgga gagggcgacc
     6301 ggcgttgatt tgccgagtta tggcggtgtc gtagcgaact cttatctgcc atcaaactgg
     6361 ctttctgcgt tggggctgta tgcctgggcg cgggtcgatg aatcttccga taataattca
     6421 ttgcttaatc cggcgaagaa attcacttat caggcaccgc aaaacgttga tgacacttat
     6481 gtcgtgttta tcatcggtga aaccacgcgt tgggaccata tgggcatttt cggctatgag
     6541 cgtaatacca cgccgaaact ggcccaggag aaaaatctgg cggcgttccg tggttactcc
     6601 tgtgataccg caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg gggcgcggaa
     6661 gataatccgc agcgcacatt aaaagaacag aacattttcg cggttctgaa gcagttagga
     6721 ttcagttctg acctctacgc tatgcagagc gaaatgtggt tctacagcaa cacgatggcg
     6781 gacaacattg cttatcgtga gcagattggt gcggagccac gtaatcgtgg caagccggta
     6841 gatgatatgt tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc ggatggtaag
     6901 catctgatca ttctgcatac caaaggttcg cattttaact acacccagcg ttatccgcgt
     6961 agcttcgcgc agtggaagcc ggaatgtatt ggtgttgata gcggctgtac caaagcgcag
     7021 atgatcaact cctatgacaa ctcggtgacc tatgtggatc actttatctc cagcgtgatt
     7081 gatcaggttc gcgataagaa agcgattgtg ttctacgcag ctgaccacgg tgagtcaatt
     7141 aatgaacgcg agcacctgca cggcacgccg cgtgaactgg caccgccgga gcagttccgc
     7201 gtaccgatga tggtctggat gtcagataaa tatctggaaa atccggccaa tgctcaggca
     7261 tttgcgcagc tgaaaaaaga agccgacatg aaagtgccac gccgtcatgt agagctgtac
     7321 gacaccatca tgggttgtct tggctatact tcaccggatg gtggaattaa cgaaaacaac
     7381 aactggtgtc acatcccgca gacaaaagtg gcagcggcta actaagcggc ctgctgactt
     7441 tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta
     7501 agatgcgccc cgcattcggt gattggcgca gcctggtagc gcacttcgtt cgggacgaag
     7561 gggtcggagg ttcgaatcct ctatcaccga ccaaattcga aaagcctgct cagcgagcag
     7621 gcttttttgc atttgaagcc catgaggatg agaacctccg ggggcgggga ggttcgactc
     7681 gagcgaagcg agagaacgtt gcgccagcaa cggcccgcag ggcgagccac gaagtggcga
     7741 gtaatcctct atcaccgacc aaattcgaaa agcctgctca acgagcaggc ttttttgcat
     7801 ttgtagctca tgaggatgag aacctccggg ggcggggagg ttcgactcga cgtaggcctg
     7861 ataagacgcg tcagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg atgcggcgtg
     7921 aacgccttat tcgacctaca cgccacacat ttccctgcca aatttgcgcc ccatatctca
     7981 ctatccatta acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatctt
     8041 tttttgcgtt acttatggct gaccctggca ttttccgctg tgcgttttag cgttcatggt
     8101 attaatcgct cagatatcca ccttttcctc agattttttt cattaaatgc ataaaactta
     8161 atcaccaaat gttgctaaat attaagctga ttgttctggt catgttggag tagcacaaat
     8221 ttctctgctg caaatagctg tttgaagttt ttttaatctt tgtttgtgga ttctcaccgt
     8281 tggtgtaaat cccggttggc tgtattgacg ttttcacatt ctgttgacag attgtaggtc
     8341 acgaggggca ttttatggag gatccgcact gttacactga tgttaattag tacggcatcc
     8401 ccacctcata acgttgaccc gaccgggcaa aaaacaaaaa aggtcaggca gcgacaaccc
     8461 actgcaaagg gttaaaacaa caaacatcac aattggagca gaataatgcg tatttccttg
     8521 aaaaattcag ggatgctgaa gcttggtctc agcctggtgg ctatgaccgt cgcagcaagt
     8581 gttcaggcta aaactctggt ttattgctca gaaggatctc cggaagggtt taacccgcag
     8641 ctgtttacct ccggcaccac ctatgacgcc tcttccgtac cgctttataa ccgcctggtt
     8701 gaatttaaaa tcggcaccac tgaagtgatc ccgggcctcg ctgaaaagtg ggaagtcagc
     8761 gaagacggta aaacctatac cttccatctg cgtaaaggtg tgaagtggca tgacaataaa
     8821 gaattcaaac cgacgcgcga actgaacgcc gatgacgtgg tgttctcgtt cgatcgtcag
     8881 aaaaacgcgc aaaacccgta ccataaagtt tctggcggta gctacgaata cttcgaaggc
     8941 atgggcttgc cggagctgat cagcgaagtg aaaaaggtgg acgacaacac agttcagttc
     9001 gtgctgactc gcccggaagc gccgttcctc gctgatctgg caatggactt cgcctctatt
     9061 ctgtcaaaag aatatgctga tgcgatgatg aaagccggta caccggaaaa actggacctc
     9121 aacccaatcg gaaccggtcc attccagtta cagcagtacc aaaaagattc ccgtatccgc
     9181 tacaaagcgt ttgatggcta ctggggcacc aaaccgcaga tcgatacgct ggtcttctct
     9241 attacccctg atgcttccgt gcgttacgcg aaattgcaga agaatgaatg ccaggtgatg
     9301 ccgtacccga acccggcaga tatcgctcgc atgaagcagg ataaatccat caacctgatg
     9361 gaaatgccgg ggctgaacgt cggctacctc tcgtataacg tgcagaaaaa accgctggat
     9421 gacgtgaaag ttcgccaggc actgacctac gcggtgaaca aagacgcgat catcaaagcg
     9481 gtttatcagg gcgcgggtgt atcagcgaaa aacctgatcc cgccaaccat gtggggctat
     9541 aacgacgacg ttcaggatta cacctacgat cctgaaaaag cgaaagcctt gctgaaagaa
     9601 gcgggtctgg aaaaaggttt ctccatcgac ctgtgggcga tgccggtaca acgtccgtat
     9661 aacccgaacg ctcgccgcat ggcggagatg attcaggcag actgggcgaa agtgggcgtg
     9721 caggccaaaa tcgtcaccta cgaatggggt gagtacctca agcgtgcgaa agatggcgaa
     9781 catcagacgg taatgatggg ctggactggc gataacgggg acccggataa cttcttcgcc
     9841 accctgttca gctgcgccgc ctctgaacaa ggttctaact actcaaaatg gtgctacaaa
     9901 ccgtttgaag atctgattca accggcgcgt gctaccgacg accataacaa acgcgttgaa
     9961 ctgtacaagc aagcgcaggt ggtgatgcac gatcaggctc cggcactgat catcgctcac
    10021 tccaccgtgt ttgaaccggt acgcaaagaa gtcaaaggct atgtggttga tccattaggc
    10081 aaacatcact tcgaaaacgt ctctatcgaa taattaaaag ccatacaaga ctgatggcaa
    10141 aggcaaaact gcctgatgtg ctccgcttat caggcctacg aaaattctgc aatgtattga
    10201 atttgcacga ttttgtaggc cggataaggc gttaacgccg catccggcat aaacaaagcg
    10261 cactttgtca acaatctgta tacccggtgg cgctgtgcct ctgcgtgcag cgccatccgg
    10321 cagcaatact tcattctctg tccgacaagg gcgggaattg atttgtgagc aatacagaca
    10381 cgcagttcca ggctgcgggt cactatagag aatccgggtt atgttgcagt ttattctccg
    10441 acgtttggga ctcgtcatcc ccacgtttat cggtattacc cttctcacat ttgcctttgt
    10501 ccacatgatc ccgggcgatc cggtgatgat catggcaggc gaacgtggga tctcccctga
    10561 gcgtcacgcg cagctgctgg ctgaactcgg cttagataaa ccgatgtggc agcagtatct
    10621 ccattacatt tggggcgtaa tgcacggcga tctaggcatt tcaatgaaaa gccgaattcc
    10681 agtatgggaa gagttcgtgc cgcgctttca ggccacgctg gaacttggcg tctgcgcgat
    10741 gatttttgct acggcagtcg gtattccggt tggtgtgctg gctgcggtta aacgcggttc
    10801 cattttcgat cacacagcgg ttggcctggc gctgaccggc tactcgatgc cgatcttctg
    10861 gtggggcatg atgctgatca tgctggtttc ggtgcactgg aacctgacgc ccgtctccgg
    10921 tcgcgtgagc gatatggtgt tcctcgatga ctccaatccg ttaaccggtt ttatgctaat
    10981 cgacaccgcc atctggggtg aagacggcaa ctttatcgat gccgtcgccc atatgatctt
    11041 gcctgccatt gtgctgggta ctattccgct ggcggtcatt gtgcgtatga cacgctcctc
    11101 gatgctggaa gtgctgggcg aggattacat ccgcaccgcg cgcgccaaag ggctaacccg
    11161 catgcgggtg attatcgtcc atgcgctgcg taacgcgatg ctgccggtgg tgaccgttat
    11221 cggcctgcag gtgggaacat tgctggcggg ggcgattctg accgaaacca tcttctcgtg
    11281 gcccggtctg ggacgctggt tgattgacgc actgcaacgc cgcgactatc cggtagtgca
    11341 gggcggcgta ttgctggtgg cgacgatgat tatcctcgtc aacttgctgg tcgatctgct
    11401 gtacggcgtg gtgaacccgc gtattcgtca taagaagtaa ggggacatca tgtcacaggt
    11461 tactgaaaat aaagtgatta gcgcaccggt gccgatgacc ccgttacagg agttctggca
    11521 ctattttaaa cgcaacaaag gtgcggtcgt cgggctggtt tacgtcgtca tcgtgctgtt
    11581 catcgcgatc tttgccaact ggattgcacc ctataacccg gcggaacagt tccgcgatgc
    11641 actgctcgcc ccgccagcct ggcaggaagg cggcagcatg gcgcacttgc tgggcaccga
    11701 tgacgtaggc cgtgatgtgc tgtcgcgcct gatgtacggt gcgcgcctgt cgctgctggt
    11761 tggctgtctg gtagttgtgt tatcgctgat tatgggcgtt attctcggcc tgatcgccgg
    11821 ttactttggc ggcctggtca ataacatcat tatgcgcgtg gtcgatatca tgctggcgct
    11881 gccaagtctg ctgctggcgc tggtgctggt ggcaattttc ggcccgtcga ttggtaacgc
    11941 cgcgctggca ctgaccttcg ttgccttgcc gcactatgtg cgcttaaccc gcgccgccgt
    12001 gctggtggaa gttaaccgcg attacgtcac cgcgtctcgc gtggcgggtg ccggggcgat
    12061 gcgtcagatg tttattaaca tcttcccgaa ctgccttgcg ccgctgattg ttcaggcgtc
    12121 gctcggtttc tctaacgcca ttctcgatat ggctgctctt ggtttcctcg gcatgggggc
    12181 acagccgcca acgcctgagt ggggcaccat gctctccgac gtgttgcagt tcgcgcaaag
    12241 cgcctggtgg gtcgtgacct tcccgggtct ggcgatcctg ctgacggtgc tggcatttaa
    12301 cctgatgggt gacggtctgc gtgacgcgct cgatcccaaa ctgaagcagt aagaggttcg
    12361 agatggcgtt attaaatgta gataaattat cggtgcattt cggcgacgaa agcgcgccgt
    12421 tccgcgccgt agaccgcatc agctacagcg taaaacaggg cgaagtggtc gggattgtgg
    12481 gtgagtccgg ctccggtaag tcggtcagtt cactggcgat tatggggctg attgattatc
    12541 cgggccgcgt aatggcagaa aaactggagt ttaacggcca ggatttgcag cgtatctcag
    12601 aaaaagagcg ccgcaacctg gtgggtgccg aagtggcgat gatcttccag gacccgatga
    12661 ccagccttaa cccgtgctac accgtgggtt tccagattat ggaagcgatt aaggtgcatc
    12721 agggcggcaa caaaagtacc cgccgtcagc gagcgattga cctgctgaat caggtcggta
    12781 ttcccgatcc ggcatcgcgt ctggatgttt acccgcatca gctttccggc ggcatgagcc
    12841 agcgcgtgat gatcgccatg gcgattgcct gtcggccaaa actgctgatt gccgatgaac
    12901 caaccactgc gctggacgtg accattcagg cgcaaatcat cgaactactg ctggagctac
    12961 agcagaaaga gaacatggcg ctggtgttaa ttacccatga cctggcgctg gtggcggaag
    13021 cggcacataa aatcatcgtg atgtatgcag gccaggtggt ggaaaccggt gatgcgcacg
    13081 ccatcttcca tgcgccgcgt cacccgtata ctcaggcatt gctgcgtgcg ctgccagaat
    13141 ttgctcagga caaagaacgt ctggcgtcgt tgccaggtgt cgttcccggc aagtacgacc
    13201 gcccgaacgg ctgcctgctt aacccgcgct gcccctatgc cactgacaga tgtcgcgctg
    13261 aagaaccggc gctgaatatg ctcgctgacg ggcgtcagtc caaatgccat tacccacttg
    13321 atgatgccgg gaggccgaca ctatgagtac gcaagaggcc accctgcaac aaccgctgtt
    13381 gcaggctatc gacctgaaaa aacattatcc ggtgaagaaa ggcatgttcg cgccggaacg
    13441 tctggttaaa gcgctggatg gcgtttcgtt taaccttgaa cgtggcaaaa cgctggcagt
    13501 agtgggcgaa tctggctgcg gtaaatcgac cctcggtcgg ttgctgacga tgatcgaaac
    13561 gcccaccggt ggtgagctgt attaccaggg gcaggatctg ctcaagcacg atccgcaggc
    13621 gcagaagctg cgtcggcaga aaatccagat tgtcttccag aacccttacg gttcgctgaa
    13681 tccgcgtaaa aaagttgggc aaattctcga agagccgctg cttattaaca ccagcttaag
    13741 caaagaacag cgtcgggaaa aagccctgtc gatgatggcg aaagtcggcc tgaaaaccga
    13801 gcactatgac cgctatccgc atatgttctc cggcggacaa cgtcagcgta tcgctattgc
    13861 ccgtggtctg atgctcgacc cggatgtggt gattgccgat gagccggttt ctgcgctgga
    13921 cgtctcagtg cgtgcgcagg tgctgaatct gatgatggat ttgcagcagg aactggggct
    13981 ttcttatgtc tttatctcgc acgacctgtc ggttgtggag cacattgccg atgaagtgat
    14041 ggtgatgtac ctgggccgct gcgtggagaa ggggacaaag gaccaaatct tcaataaccc
    14101 gcgtcatcct tacactcagg cgctgctctc cgcgacgccg cgcctgaacc cggacgatcg
    14161 ccgcgagcgc atcaagctca ccggtgaact gccaagcccg ctcaatccac cgccgggttg
    14221 cgccttcaac gcccgctgtc gtcgacgctt cggcccctgc acccagttgc agccagcact
    14281 gaaagattac ggcggtcaac tggtggcctg ctttgccgtt gatcaggatg aaaatccgca
    14341 gcggtaatgt tcaaaaccgg agccttgtgc tccggtttac tcaatcagct tcaacagtgg
    14401 cgacaagcat agcaaaatgc cgtacagcag aatcagccag gctttaaatc cgcgaagttt
    14461 ttccagttgt gcgactttat agatcaggaa aaacggaatg aggcacgaca caatgccata
    14521 taacgggctt cccaactgaa agaacaccag caccgataca cgaaacgaaa cccaaatcgt
    14581 caacgtaatg acgataaaag cgcagatcgc cagagtcagc atgcgtgagt taattttctt
    14641 ggtatcaata attcggctta acagattgag aataatgcct ttaatagcct cgtggaaacc
    14701 gagataaatg ccaaagaatg cggtcagtac ggcaaagata ttcagcaccg tagaggtgat
    14761 atgaatgata tgtccaggga tcacctgcgc ggccagcgcc agtgccgaga tattttgttc
    14821 aaaggcagaa acggcttctt cgtggctaat tgagaaggta aacgaaaagg caaaaaacag
    14881 gatcaccgcg atgagcgtga tataactaat tcggtgggta cgcagcgcga gccgggttgc
    14941 cagtacttta tctgcttccc gtttacgata ggcaatattc attgggttaa gtacctgaat
    15001 aaataccgca gaaaagaagc aaaatggaat ggtaagcagg acatcgcgga aaaagacgga
    15061 ggcttgcggg aaggcggtta tattggcgaa attccagtgc gggatcatcg caaaaccgaa
    15121 cacgacaata atccctactt tgaccaccac cattggccca gaaatcttaa acagtaatcg
    15181 ttcaccacca gacgcaatcg ccaccagtac ggcgaaaata gcgactttat aaagtagaga
    15241 ttgtgaaaga tcggcatcgg ttaaaccgaa ggttttcagg tacgaggcgc tgtcgaaaac
    15301 cacggagaga gagtagataa aaatcccgtg gataatcatc aaaaagtaga taaccccgag
    15361 gaaaattccc cagttcttcc ccaggtaatg actgataata tcggtgtagt cattacagga
    15421 atcgctttca gaaagggttt ttaaataaat gtcctgcact acccaggtgg caggataagc
    15481 aatgatcgct gcggtaataa atacccaaat tcccttcaag ccaatttgta ctggcatcag
    15541 cacggttccg gcaccaatcg ccatgcctat gcataataaa acccagccga agtcgtagcg
    15601 tgtaaacggc aatttctgat tgtgtttcga tagtgtgttg tgctgcataa agtcctgtcc
    15661 agaaagaagt cggcctcgca aaaatgaata aacccagttt ctcttgcgca acaggtggag
    15721 aagtcgaata cgcagtatgt aaaaaatgag gcaacagatt cactggggag gtcgcgagga
    15781 ggcgcaattc tacaaaattg cgcatttcac taccagtctg gagtcagccc ggatatgaac
    15841 tgcaactatt gcaaataaag aaattaagct ggaaggcatc gcgcaatgag gaagattgcc
    15901 gggtgctgac agtaaaaagc aatcgtaaaa tcagcggcaa gccggaaggg ttccggtgag
    15961 gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggagaga gaaaaccccc
    16021 gcacgttgca ggtatgtacc tgacaacata acgggggcta atcttgaacc tagaccactc
    16081 aagaatagcc gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc tcgcagagct
    16141 gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggcattc ttgccagtat
    16201 gatcgtgaac tggctgaaca agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa
    16261 tggcaatttg cgcccggacc aggccgcagg ggtgaaactc tgcggccttt ttcgtgctta
    16321 ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg tacggtttat cctggtattg
    16381 aataactact gcatttgagt tctcggagac cggtgctgtt tgtggcaacc cactggtgag
    16441 ttttttccag tcaacattgt cttcggtgaa aatcttgcca tcaagaacgc gaaccaccag
    16501 atcggagata gccaggaagc tgctcggttg gtcaatgaca atcggtgccc cctgatgtgg
    16561 tgccttcatg ccgaagaatt tcaccccaac ggggacgtcg gtgatagacg ggctagggat
    16621 atcacgtagg ccagatacct gcattctgtc gcccttcagc gcgccgccgt gttccggcac
    16681 cacgaccacc atcactttac gacccgattt ctccagttca gtaaagaagg cgtccagttc
    16741 atcaaagaat ttctgcgccc gcgctttgta atccgctgtt ttgctgaccc ccggataatg
    16801 gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca ctacggctat ttttatcttt
    16861 ttcggtaacg tccagccagc ggttaagcac ggcggtatcg tcataaaccg gcgaaccatc
    16921 aaagcccagc aaaataaccg gcagatttgt ttgatccatc aattcagtct gcatgccgcc
    16981 attttcgcga acttctttca aaaaaccgcc gaactggccg ttatgcccca tcatcaggtg
    17041 ctgggtaaag cccagtttcg aaaggttatc aaacagatag cagtcgttat ttgccggttg
    17101 atacagatta gtgtgcgaag tctgcccgca gctggcgcgc agtaaacgga tcgccgccgg
    17161 accactgtag gaggtggcgg agttaaagtt cttgaactca atatcgaaat gcgaccacag
    17221 tggatgcgac atcaacccgg cggcttctat atccgaccag gaaagcgaac agatgttaat
    17281 caccagtagt tcaaatggct gagcatcagc gggcagcgaa gacgggaagg tcgatttacg
    17341 tttcgcctcc gcgttataga aattattcag ccaggcgtta aggttcgccg ttgttggcgg
    17401 tgcagtttgt gcgggcatat cacccactac cggtgcgcca cccgtcgccg caacggttgc
    17461 cgctgcgtta ccacccgtcg ttgttacagt ggtcgtcggt tgtccggctg gccacaagga
    17521 gaaacttggt cccgccaggg taagtacgtt cagccatagc agtatggcaa ccacaaaaac
    17581 ggtaatgcga atccattgtg acaggaataa ccaggccact aataaaacaa aaatggcccc
    17641 aatcatctgc cagttaataa agcgtgtgac aaggtcgatt aaataatcgg tactgaaccc
    17701 cgccacctgc gaaccctggc tcattatgct ttccgggcca ggcaaccagg tgtcatgcca
    17761 gaacaaagca aagccgatcg gcagggcaat ccagtggcgc aagcgatgca ggctgtagcg
    17821 cggaatgggc atcagcagaa acgcggcaaa caccaaattg aggagcggat ggaagttaag
    17881 atatcccgcc cacaacaggc cgaacttaac cagaaaatag aagttccagc cggaaaggcc
    17941 gcgccagtat tgccagaggg aagaaggcat ggcggtattt tgtgtaaatt gagtcatttt
    18001 ttggttgccc tggctttttc cgtgcggcgt aacgtcccgg ccggtttaac ataacgaggt
    18061 ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca aagagtgccg cgccagatag
    18121 cccagcggga aaaatatcag tgcgcaaacg acaataattt caatgatatc gctgatggtc
    18181 atcatgatga gcgctccaca gcatcatcta acagtcgcat gggttctggt attcgtcgcc
    18241 agtggcgacc atcgtgctcg gcattgatga ccggtttaga actttgcgtt aaaggcagcg
    18301 gcatgcccca ttgttctggg gcaagcaagc gcatctgcac cagctcggca ctgatttgat
    18361 catcttcaaa ccagaccata cggtttgaga aaatgtcgcc agtaggcaat gggaaaatat
    18421 gattcaacgc ggtatccaga tcgttaatcc gacagaatga gagaaacagc accagccgat
    18481 taccgccaat ggtcatgata tcgccggtac ggttagggcg acacagcgtc agggcttgtt
    18541 caacgcggat acccggtacc ggacgtaggg caaccagaac gcctttaccg tgggcaggta
    18601 atagagggtt attcatcatg ttgttgacgg cattacagaa cacatcccac ttctggaaac
    18661 cacgcagttt gagcggctgg gtcattgaca gcaaggtagt gatatcttcc ggcacatagc
    18721 gactaaactt ctgcccttgc acgctttcga tcatcgtcag acaacgggag agtggcgcat
    18781 tccacggaat aaccatattt gcaccgcagg ccaataacaa acgttcatcg gtggcgcgca
    18841 ggctagcggt attttcccgc acgaggattt tcatcgcact accgcgctgg cgacgcaggg
    18901 tatgaatgct gcgggccagt ggctcgattt gcgcattttg ctgtaaagaa aagaccaccg
    18961 tcgccgcctg agcggtacgg gcttcattga acaggacttc gttattgttg aacagttgcc
    19021 agtgttccga tagcggcggc gcaccttcca gtacagcaac attactcaga atgcgttttt
    19081 cgtcgctgcg tggttggatc tccgcctctt cgctttgaac taatgtccag ataccatttt
    19141 gttgctgaac gctaagctgc tgacgggcgc tgaccccttt ttcgttgcac cagaaggcaa
    19201 tatccagcaa atgttggtca ccctgaaaac gcaaactggc aagaccaaaa agtgaacggt
    19261 attcctcaag caacaatgaa aattgtttat cgttattatt tccgggatta attaccaaaa
    19321 gcgaacaatg gtttaacctg ctccatttat tcattttatc caaccatgag cgaagccgct
    19381 cggcaggaat gttttgccat gcgttatttg cgcaaacaag aataaaaagg taattatggg
    19441 gatcaataga acactgcaaa tcacgggtca aatagtatag acctttttca tgatttagca
    19501 ttgaaaataa ttttattttt tccggccctt gagaatcatc taattgaaag attttcgccg
    19561 gatcgctgtc catgctaatg accgcgacgt gtgcggtttc agcctgggat gcaattgttt
    19621 gattcgccag actgatagca tcttcatggc gatcgacgtt aaaccaccag acgccgcctg
    19681 ctggcatatg tcgcagctca tcccataatg atgagatacc gatagagaat acagggtcca
    19741 caatgtccct catgtcacca ttttgtctga ttatcagttt actagcgaaa gctaagaaat
    19801 aaacctacca ttgaaattaa aacttatcag atttagcatg tgaatcaatt tagaaggtca
    19861 caagacgtgt gattttagtc ttttcgtttt taactttttc tgttgcgtaa cggaaagtca
    19921 aaaagtgagc aaattcccgt ctcgccgtaa acaataaacc ggcgaaatgc tcacggttag
    19981 aattgtctca tgaacggtac ggttatttca tagggatcaa gcaaaatgaa taacaatgaa
    20041 ccagatactc tgcctgatcc cgcgataggc tatatcttcc agaatgatat tgtggcgtta
    20101 aagcaggcat tttcactgcc tgatattgat tatgccgata tttcccaacg cgaacagttg
    20161 gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt
    20221 ggtgaatggc cgtactggga ttgcaggggg tgcggggagg cgtggggaca acaaccatca
    20281 ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgttctggtg gtcgatgcct
    20341 gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt cagggctggg
    20401 ccagagcgat gctggatggc caggactggc gtgacgctgg gttgcgctac acctcgcagc
    20461 tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcactggc
    20521 aaacccggct gagcgatatt tgctccggct tacagtaact aaaagccagc gggcgttacc
    20581 agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt
    20641 tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc cgactgcatc
    20701 agcaagcgct gccggatggc gcacatattt tgattaatga cttccgtatt ggcagtcagg
    20761 ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattactg ccgatgctca
    20821 ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagta ggtgaatatc
    20881 gcagtgatgc gctggcggct gaagagatac tgacgctggc gaactggtgc ctgttgaact
    20941 actccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct
    21001 tatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc gtcacggtgc
    21061 gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc
    21121 gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atccgcatat
    21181 caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa catgctggtt
    21241 actgatcggt acatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat tttcaggtct
    21301 gcagaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc gcgttagcca
    21361 taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg
    21421 gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct gcgttactca
    21481 accgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtggggtg tagcgctgat
    21541 cgtacggcgg atgccggggc gcttctcggc gctaatgttg attgtgctgt cgctgaccgt
    21601 ttcttgccgt tatatctggt ggcgttacac ctctacgctg aactgggacg atccggtcag
    21661 cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct
    21721 cggctacttc caggtagtat ggccgctgaa tcgtcagccg gtgccattgc cgaaagatat
    21781 gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc tcaacgtggt
    21841 gaaaaatact atttacgcct cgctgggtat cgactggccg aaagacaagc tgaacatctg
    21901 gatcctcgat gacggcggta gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa
    21961 atatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa
    22021 atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt
    22081 cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga tgcagacgcc
    22141 gcaccacttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc gtaaaacgcc
    22201 gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt gggacgccac
    22261 tttcttctgc ggttcctgtg cagtaattcg tcgcaaacca ttggatgaaa ttggcggtat
    22321 tgctgtcgaa acggtaaccg aagatgcgca cacgtctctg cgcctgcacc gtcgtggcta
    22381 cacctctgca tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc
    22441 gcatatcggt cagcgtattc gctgggcacg cgggatggtg caaatcttcc gtctcgataa
    22501 cccgcttacc ggtaaagggc tgaaattcgc ccagcggctg tgctacgtca acgccatgtt
    22561 ccacttcttg tcgggcattc cacgactgat cttcctgact gcgccgctgg cgttcctgct
    22621 gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctgttcg tgctgccgca
    22681 tatgatccat gccagcctga ccaactccaa gatccagggc aaatatcgcc actctttctg
    22741 gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct
    22801 gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg tggaagaaga
    22861 gtacgtcgac tgggtgatct cgcggcccta catcttcctt gtcctgctca acctggtggg
    22921 cgttgcggta ggcatctggc gctacttcta tggcccgcca accgagatgc tcaccgtggt
    22981 cgtcagtatg gtgtgggtat tctacaacct gattgttctt ggcggcgcag ttgcggtatc
    23041 ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc ccgcggcaat
    23101 tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg acggtggttt
    23161 ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga atctgttgct
    23221 taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga
    23281 agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg tgcagtgtac
    23341 gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag ataagccgct
    23401 ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc tggcggagtt
    23461 tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt cctgggttgt
    23521 atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg atcaggcttt
    23581 ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta
    23641 tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg caaccactga
    23701 tcaatgctga gccagctgta gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga
    23761 tgccgggcgt gcagggcgct gatgcgccag tcgtggcgca gaacggtcct tcgcgtgatg
    23821 tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta cgtggcatta
    23881 acccgaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg aaggcgatgc
    23941 tcaacctcga atacacgcca tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt
    24001 atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa
    24061 cgctggcgca aatgcccatt aacccactgt ttattaccga cttcaaccgt gtgcggctgg
    24121 agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg
    24181 atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg aagaatgacc
    24241 tgtcacactt cccggtgcca ttctttgatc cgcgtgataa ccgcaccaac accttgccga
    24301 tggtctttgc gggtgcgccg gatgttgggc tgcaacaagc ctctgccatt gtcgcctcgt
    24361 ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat aaccaactgc
    24421 cggatcgtaa tgccattgtc tttgcaacca acgacaaacg gccggacttc ctgcgcgatc
    24481 atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat ccttacgtca
    24541 aactgctggt ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg
    24601 ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc
    24661 taccgcgtaa gccgtacgat gcgccgaact gggtacgtac cgatcgtccg gtcacatttg
    24721 gcgaactgaa aacctatgaa gaacagttac aatccagcgg tcttgagcca gcagcgatta
    24781 acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc attgatatgg
    24841 atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg gatatcagcc
    24901 tgaataacca gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg aaccgcctgc
    24961 tgctgcggat tccggtatta caaggtttgc tggatggcaa aacagatgtc tctattccgg
    25021 cgctgaaact gggcgcgacc aaccagctgc gcttcgactt tgagtatatg aacccgatgc
    25081 cgggcggttc ggtggataac tgtattacct tccagccggt gcagaatcat gtggtgattg
    25141 gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg ccggatctac
    25201 gcgcctttgc taacgcgggc ttcccattca gccggatggc ggatctgtcg caaaccatca
    25261 ccgtgatgcc gaaagcgcct aacgaagcac agatggaaac gttgctgaat actgttggtt
    25321 ttatcggcgc acagacgggc ttcccggcga ttaatctgac ggtgaccgat gatggcagca
    25381 ccattcaggg caaagatgcc gacatcatga tcatcggtgg tatcccggac aaactgaaag
    25441 acgataagca gatcgaccta ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc
    25501 gccagacccc gttccccggc attgtgccgg acgagagcga tcgcgcggca gaaacccagt
    25561 caacgctgac ctcttccggt gcgatggcgg cggtgattgg cttccagtcg ccgtataacg
    25621 accagcgcag cgtgattgcg ctgctggcag atagcccacg cggttatgaa atgcttaacg
    25681 atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg
    25741 agtccggtat caacagccta cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt
    25801 tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg ctggcggcta
    25861 tcagtgtgat attgctggca tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc
    25921 gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga
    25981 tgctgctg
//
LOCUS       DAFATA010000061        24678 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFATA010000061 DAFATA010000000
VERSION     DAFATA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.062"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            263..1123
                     /gene="mscS"
                     /locus_tag="LGJ20_002982"
     CDS             263..1123
                     /gene="mscS"
                     /locus_tag="LGJ20_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBK2387013.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            1262..1897
                     /gene="argO"
                     /locus_tag="LGJ20_002983"
     CDS             1262..1897
                     /gene="argO"
                     /locus_tag="LGJ20_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBK2387014.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            1990..2730
                     /locus_tag="LGJ20_002984"
     CDS             1990..2730
                     /locus_tag="LGJ20_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBK2387015.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            2897..3793
                     /locus_tag="LGJ20_002985"
     CDS             2897..3793
                     /locus_tag="LGJ20_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2387016.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(3790..5268)
                     /gene="scpC"
                     /locus_tag="LGJ20_002986"
     CDS             complement(3790..5268)
                     /gene="scpC"
                     /locus_tag="LGJ20_002986"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBK2387017.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(5292..6077)
                     /gene="scpB"
                     /locus_tag="LGJ20_002987"
     CDS             complement(5292..6077)
                     /gene="scpB"
                     /locus_tag="LGJ20_002987"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBK2387018.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(6088..7083)
                     /gene="meaB"
                     /locus_tag="LGJ20_002988"
     CDS             complement(6088..7083)
                     /gene="meaB"
                     /locus_tag="LGJ20_002988"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBK2387019.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPSHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(7076..9220)
                     /gene="scpA"
                     /locus_tag="LGJ20_002989"
     CDS             complement(7076..9220)
                     /gene="scpA"
                     /locus_tag="LGJ20_002989"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBK2387020.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLESVRDVLNLISQHHD"
     gene            complement(9424..10317)
                     /gene="argP"
                     /locus_tag="LGJ20_002990"
     CDS             complement(9424..10317)
                     /gene="argP"
                     /locus_tag="LGJ20_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBK2387021.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10459..10644
                     /locus_tag="LGJ20_002991"
     CDS             10459..10644
                     /locus_tag="LGJ20_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387022.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            10700..11359
                     /gene="rpiA"
                     /locus_tag="LGJ20_002992"
     CDS             10700..11359
                     /gene="rpiA"
                     /locus_tag="LGJ20_002992"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK2387023.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            11615..12847
                     /gene="serA"
                     /locus_tag="LGJ20_002993"
     CDS             11615..12847
                     /gene="serA"
                     /locus_tag="LGJ20_002993"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK2387024.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            13076..13135
                     /gene="ibsC"
                     /locus_tag="LGJ20_002994"
     CDS             13076..13135
                     /gene="ibsC"
                     /locus_tag="LGJ20_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBK2387025.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            13468..13527
                     /locus_tag="LGJ20_002995"
     CDS             13468..13527
                     /locus_tag="LGJ20_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBK2387026.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(13628..14230)
                     /locus_tag="LGJ20_002996"
     CDS             complement(13628..14230)
                     /locus_tag="LGJ20_002996"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK2387027.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(14251..14434)
                     /gene="ssrS"
                     /locus_tag="LGJ20_002997"
     ncRNA           complement(14251..14434)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LGJ20_002997"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(14476..14805)
                     /gene="zapA"
                     /locus_tag="LGJ20_002998"
     CDS             complement(14476..14805)
                     /gene="zapA"
                     /locus_tag="LGJ20_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK2387028.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            14973..15551
                     /gene="ygfB"
                     /locus_tag="LGJ20_002999"
     CDS             14973..15551
                     /gene="ygfB"
                     /locus_tag="LGJ20_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBK2387029.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSGTSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            15577..16902
                     /gene="pepP"
                     /locus_tag="LGJ20_003000"
     CDS             15577..16902
                     /gene="pepP"
                     /locus_tag="LGJ20_003000"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBK2387030.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            16899..18077
                     /gene="ubiH"
                     /locus_tag="LGJ20_003001"
                     /gene_synonym="visB"
     CDS             16899..18077
                     /gene="ubiH"
                     /locus_tag="LGJ20_003001"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBK2387031.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREELLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            18100..19302
                     /gene="ubiI"
                     /locus_tag="LGJ20_003002"
                     /gene_synonym="visC"
     CDS             18100..19302
                     /gene="ubiI"
                     /locus_tag="LGJ20_003002"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBK2387032.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWSKAQQASDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     HQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSSALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(19376..19459)
                     /locus_tag="LGJ20_003003"
     CDS             complement(19376..19459)
                     /locus_tag="LGJ20_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387033.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            19749..20843
                     /gene="gcvT"
                     /locus_tag="LGJ20_003004"
     CDS             19749..20843
                     /gene="gcvT"
                     /locus_tag="LGJ20_003004"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBK2387034.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            20867..21256
                     /gene="gcvH"
                     /locus_tag="LGJ20_003005"
     CDS             20867..21256
                     /gene="gcvH"
                     /locus_tag="LGJ20_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBK2387035.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            21375..24248
                     /gene="gcvP"
                     /locus_tag="LGJ20_003006"
     CDS             21375..24248
                     /gene="gcvP"
                     /locus_tag="LGJ20_003006"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBK2387036.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            24509..>24678
                     /locus_tag="LGJ20_003007"
     CDS             24509..>24678
                     /locus_tag="LGJ20_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK2387037.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVL"
BASE COUNT         5788 a         6200 c         6671 g         6019 t
ORIGIN      
        1 gtatcctgca atatattgaa tttgcaggtt tttgtaggcc ggataaggcg ttcacgccgc
       61 atccggcatg acaaagcgca cattgatatc tgtctgagcc ccgctctgcg gggatttttt
      121 cgccaaaagc aaggtgattc agatgagaaa tctgtgatct atttggcaaa attatgcttt
      181 attgtttacc cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc tccattttga
      241 gtcagttgaa aaggaatatt gaatggaaga tttgaatgtt gtcgatagca taaacggcgc
      301 gggaagctgg ctggtagcta accaggcgct gctgctaagt tatgcagtaa acatcgtggc
      361 ggcactcgcg atcatcatcg ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa
      421 tcgcctgatg atctcccgta aaatcgatgc cactgttgct gattttctct ctgcattagt
      481 ccgttacggt attatcgcct ttacgctaat cgctgcactg ggacgcgtgg gtgtacaaac
      541 cgcgtccgtc attgctgtac tcggtgccgc aggcttagct gttggtctgg ctttgcaggg
      601 gtcactgtct aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg
      661 agaatatgtt gacctcggcg gcgtagccgg tactgtgctg agtgtgcaga ttttctccac
      721 caccatgcgt actgcagacg gtaaaattat cgttattccg aacggtaaaa ttattgccgg
      781 aaatattatt aacttctccc gcgagccagt tcgccgtaac gaatttatta ttggcgtggc
      841 gtatgattcc gatatcgatc aggttaagca gatcctgacc aatattatcc agtctgaaga
      901 tcgcattttg aaagatcgcg aaatgactgt gcgcctgaac gaacttggtg catcgtcgat
      961 taatttcgtg gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt actgggatgt
     1021 gctggagcgt attaaacgtg aatttgatgc cgccggtatc agcttcccgt acccgcaaat
     1081 ggatgtgaac tttaagcggg tgaaagaaga caaagctgcg taatcaacgc tgaggccaga
     1141 taatactctc tggcctctct cttattagtt attctgattg ccaattaata ttatcaattt
     1201 ccgctaataa caatcccgcg atatagtctc tgcatcagat acttaattcg gaatatccaa
     1261 cgtgttttct tattactttc aaggtcttgc tcttggggca gctatgattc tgccgctcgg
     1321 cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt cagtatcaca ttatgattgc
     1381 cttactttgc gctatcagcg atttggtcct gatttgcgcg gggatttttg gtggtagcgc
     1441 gttattgatg cagtcgccgt ggttgctggc gctggtcacc tggggcggcg tagtcttctt
     1501 gctgtggtat ggttttggcg cttttaaaac agcgatgagt agtaatattg agttagctag
     1561 tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc accatgttgg cagtgacctg
     1621 gctgaatccg catgtttacc tggatacttt tgttgtactg ggcagccttg gcgggcagct
     1681 tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt agcgcctctt tcctgtggtt
     1741 ctttgggctg gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca
     1801 gcgcattatc aatctggtag tgggatgtgt catgtggttt atagccttgc agctggcgag
     1861 agacggtatt gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa
     1921 acagaggcgc taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga
     1981 gagactaacg tgaagttcaa agttatcgcc ctggcggcat taatgggtat tagcgggatg
     2041 gcagcgcagg ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagt
     2101 gtggatgcgg tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat
     2161 gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgaa
     2221 ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt tacgcaccca gccagattat
     2281 gattatcagg atggtaaaag tatcctgaaa ggctaccgcg ctgtgagaac ggtggaagtc
     2341 acgctccgtc agttagacaa actgaattcc ttgctggatg gcgcgctgaa ggcgggtctt
     2401 aacgaaattc gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg
     2461 cgtaaggcag cgattgataa cgcgattcat caggcgcagg aactggcgaa cggctttcat
     2521 cgtaaactgg ggccggtata tagcgtgcgc taccatgttt ccaactatca gcccagccca
     2581 atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg cccaggaaac ttacgagcag
     2641 gccgctattc agtttgatga tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa
     2701 caacccgcta aaacacctgc agcacaataa tacttgtagc ccgacgtatt cgtcgggcta
     2761 atgcctttac ccgatattgc ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc
     2821 acctatcttt gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag
     2881 gatgcgggga ggggggatgg acatttttat ttcgaaaaag atgcgcaatt tcattttatt
     2941 agcgcaaacc aataatattg cccgggcggc agaaaaaatc catatgacgg cttcgccatt
     3001 tggtaaaagc attgccgcgc tggaagaaca aattggctat acgctattta cccgcaaaga
     3061 taataatatc agcctcaata aagccggtca ggaactgtat caaaaactgt tcccggttta
     3121 tcaacgactt tctgcgattg ataatgaaat ccataactca gggcgtcgtt cacgagaaat
     3181 tgtgattggc atagataata cttatccgac aattattttc gatcagttga ttagtctcgg
     3241 agataagtat gaaggcgtga ccgcccagcc agttgagttc agcgaaaacg gtgttatcga
     3301 taatcttttt gaccgtcagt tagattttat tatctcccca cagcatgtat ctgcacgagt
     3361 tcaggaactg gaaaacctga ctatcagtga attaccaccg ctgcgattgg gctttctcgt
     3421 ttctcgccgt tatgaagagc gtcaagagca agagctgctt caggagctgc cctggctaca
     3481 aatgcgcttt caaaaccggg ctaacttcga agcaatgatt gatgcgaata tgcgaccttg
     3541 tgggatcaat cccaccatta tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt
     3601 ggagcgcgga cattttctga cggtgatccc gcattttgcc tggcgactgg tgaatccggc
     3661 aacgctgaaa tattttgatg cgccgcacag gccgatgtat atgcaggaat atctttattc
     3721 aatcagaaat catcgttata ccgccacgat gcttcagcat attgctgaag atcgtgacgg
     3781 gacaaaccat taacccagca tcgagccggt tgcaattaaa ttacggtgta agtcgaagac
     3841 gtggctaaga tcgtggtgaa tatgtccgcc gggcgcattt tccagatagc gatgcagata
     3901 atcccgatac ataggatgtg cacaattatc aatgatagtg cgggcgcgtt gaagcgggga
     3961 aagaccgcgc agatcggcga tcccttgttc ggtgatgatc actttgacgc tgtgttcgct
     4021 gtgatcaaca tggctgcaca ttggcacgac ggttgagatc ttgccttctt tagcaatcga
     4081 cggagccata aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc
     4141 gttcatcaga tcgaccccgg ctacgtgcgt tgagttggca tgcccgtaaa tatcaaactc
     4201 caggccgacg ttcagagcga tgacgcccag acgacggatg atttccgggt tattggaaat
     4261 ctcctgcgga cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg
     4321 cagggaatcg gccgagattg tcaggctgga ggcgctggcc ccgctgattt tgccggtttc
     4381 cagtaaatgc accaccgatt cctgtagcac ttccgaatac atcatgaacg gaggaatttc
     4441 cgggttttcc cccagacgcg ccattaccgc attattgata ttgcccacgc cactttgcag
     4501 cggcagaaat tccggcggaa tacgcccatg cgccatttcc tgcaataaga acgtgaccac
     4561 gttatcggca atctgctggc acatgggatt ttgcttatcc agcatattac cggcgtcggg
     4621 caagttggtt tccacgacgg cgacaatctt tttcggatcg atttgcacat agcgggtacc
     4681 gacgcgatcc attgcatgga agatcgacac gctattgcgc cgcggtggcg cgccaggaat
     4741 cacaatatct gccagttctg caacgcgcgg atcgtgatag tgattaagtt caatgatcac
     4801 tttcttcgct cgcagcagcc aggtcggcgc attaccgatc ccgctggtta accagactcg
     4861 accatccggt gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc
     4921 gtaattgacc atttgcgcca cttcgctcaa atgcaggtca acgaaactca ccgcgccctg
     4981 attgatcttt ttacgtaaac cggacgatgt ttgatatggc gcacgccagg aaacagcatc
     5041 ggcgtcagaa agtacatcgt cagcggcggc gctgattgac gcccccgtca gcaggcgaat
     5101 ttgatacggc tttttggcct catgctgttc gttagctctg cgggcaatcg cggtgggtag
     5161 ggctttcggc gaacccgccg gggtaaagcc gctaaatgcc accatgtcgt tatgctggat
     5221 aatttctgcc gcttcatcgg cggtcatcct tgtccactga gtttccattt ttactccttc
     5281 gttcgcaggg attaatgacc aacgaaatta ggtttacgtt tttcgaggaa agcgttcatt
     5341 ccttcctggt aatcttcgct gtcataaacc gcgcgtcgca tcccctgaat acgttcaaat
     5401 tcatcggagt tcatggtgtg cgcttcgccc agtacacgca gctcttcttt gataacagca
     5461 atggctaacg gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa atcttccagt
     5521 tcttccactt ccacaacatg gttgaggatg ccgacagcca acgcgcgctg ggcggtgatt
     5581 ggcgaagcgg taaaaatcag ttctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg
     5641 tgaatgccga ccaggttata cgggacgccg aggtttacag gcgtcattga gaaggttgag
     5701 gtactggcgg cgataatcag atcggaactc atgatcattt caaatgcgcc accccaaaca
     5761 ctaccttcca ccatcgaaat gatcggtttc gggaattttt ggatcatgcg ggtgatttga
     5821 cgcaatggat catcatagga gagcggatcg cgaccgccag acggcagttc gtgaatatcg
     5881 tggcctgcgg agaagacttt ggatccactc ggcgcgcgca aaatgataca gcgaatttcc
     5941 ggccggttga gatcgcttaa tgcctgcata agatcatcaa taaagacttt acttaaggca
     6001 ttaagtttgc ggccatagtt aaactcaatg accgccactt tgttgatagt gacaacgtta
     6061 acatactgat aagacataaa aattccttta atcaaaatat tgcgtctgga taaattcact
     6121 gagctgccgc aggccggtgc gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg
     6181 gcggtaatag cgatcgaaat cttcattcgc gaacaggtga tgcagtactt cttcttcggt
     6241 ctgcttacgc agccattcca ccgattgttg ttgccgcact tgttgtaaac gaccactggc
     6301 agttagcgcg gttttgaagt cgatgatggc gtgccagatc tcatcgattc cacgtttttc
     6361 cagtgcgcta caagtcagaa cccgtggctg ccattcgtcg tatttacgtc gcagaatatg
     6421 cagggcactc tcgtacatat gccgggcaat ggcgacattg gtatggttat cgccatcgtc
     6481 tttgttgata acgagcagat cagccacttc catcagccct tttttaatgc cctgcagatc
     6541 atcgccgcca ccggcaattt gcaacgagat aaaacagtcc accatgcggg cgacttccgt
     6601 ttccgactgc ccgacgccaa ccgtttcgac aatcactacg tcataacccg ccgcttcgca
     6661 taacagcatt aattcccgcg ctcgctgact ggcaccgccc agatgaccgg aggatggtac
     6721 cgggcgaata aacgccgctt cggcacgcgc caggtcattc atgcgggttt tatccccgag
     6781 aatgctaccg ccagtgaccg ggctgctggg atcgaccgca ataaccgcga cctttaatcc
     6841 ctcacgaatc aacaacatgc caaaggcttc aagaaaggta cttttccccg cgccgggtgt
     6901 gccggtaacg cccagtcgca gggcgttacc gcagtacggc ataatggcat caagcagttg
     6961 cgtacttagc gcctgatgac tcgggtgacg gctttccacc agcgtcatgg cctgggcgag
     7021 tgtggcacgc tcaccctgac gtaagcggcg aatactttct gccagcgtgg cttcattaat
     7081 catgatgctg gcttatcaga ttcagtacgt cgcgcacact ctcgagcata ggtgtacctg
     7141 gaccataaat cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc ggtggaatga
     7201 cgccaccggc gactacgcag atatcttcgc gtccccattt tttcagcgct tcgaccagtt
     7261 ccgggatcag cgttttatga ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt
     7321 tttctacggc caggcgggcg atctcttcag gtgtagagaa catcgggctt aaatctacgt
     7381 cgaaaccgag atcggaatag gcgctggcga tcactttcgc gccgcgatcg tgtccatcct
     7441 ggcccatctt agcgatcaga atgcgcgggc gacgaccatt gtcggcaagg aactgctccg
     7501 tttgcgcaac aatggcatcg aactcggagg ccgatttctc agactgatga tagctttgcg
     7561 caatcacgcc ggtaacacac tggcttggca ccagataacg gtcgaaagcg acttccagcg
     7621 catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg gcagccagca
     7681 ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat
     7741 catcacgggt ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc accatcacgt
     7801 tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa
     7861 cgatgacacg cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca
     7921 ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt
     7981 ggataatagc tctggcttgt ttgacgattt gatcggtcag cgactcaatg taataggatc
     8041 cggccagtgg atcgacggtg cggcagagtt ctgattcttc ctggatgatg atctgggtgt
     8101 tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg
     8161 tatgcagtga ctgagtaccg cccagcgtcg cagccagcgc ttcaatggtg gtgcggataa
     8221 cgttgttata cggatcctgt tcagtcaggc tccagcctga ggtctggcag tgggtacgca
     8281 gcgccagtga tttcggatcc tgtgcgccaa atccactgac cgcttcgctc cataaataac
     8341 gtgccgcacg caacatggcg acgttcataa acagatccat gccgataccg aagaagaacg
     8401 acaggcgagg agcgaagtca tcaattttca gtccggcaga gattgctgct ttgatgtact
     8461 caatcccatc agcgagcgta aatgctacct gctgcacgca gttggcaccc gcttcaccca
     8521 tgtggtaacc gctgatactg atggtattaa atcgcggcat gttgccggaa caccaggcga
     8581 tgatgtcggc gataatgcgc attgacggtt ttggcgggta aatataggtg ttgcggcaga
     8641 ggtactcttt gaggatatcg ttctgaatgg tgccggtcag tttatcaggt gtaacacctt
     8701 gctcttctgc ggcgacgata taaaacgcca gtactggtag cactgcgcca ttcatggtca
     8761 tcgaaaccga cattttatcc agcgggatct ggtcgaacag gactttcata tcttccacgg
     8821 tgtcgatagc gacgcccgct ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt
     8881 agccacggtg ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt
     8941 tacggcgata aaaagcgttg gactcttttg ctgttgaaaa accagcatac tgacggatgg
     9001 tccacggttg ggcggtatac atagtggcac gcgggccacg aacgtagggc ggcaaaccag
     9061 gaagggtacc tgtcacctcc agattatcga gatcggcttc ggtatacagc ggcttgatgg
     9121 cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt tttctcccga cggctcaatt
     9181 ccttgttggc aagctgttgc cactcctgct cgttagacat tatttgctcc gtaaaacgca
     9241 atcaaaatga gggtatttgc gcttacggtg agctttttac gtgggtttgt cgccagcaaa
     9301 aaaaatgcat tcagacgtcg ttttttgccg cctgagattt gtctattttg tgatgtgatt
     9361 caaggaatac aaaaagaagt gaggataacg cctgatatgt gctatcaggc attatttgat
     9421 ggattaatcc tgacgaagga ctttgtgacc ataatcgagc aacgcatcag tgactttacg
     9481 catcatgcgg ctttcaggag caaagcggtg ccagtagagc atccggcgtt gaaatagccc
     9541 cggcgttaag tcaatcagtt caccgctggc cagttctttc tcgatttgca ggtgcgggat
     9601 catacagcag gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag aattaacgat
     9661 atggcagggc acgctgcctg gaggcagatc gaagttttgc tgcaaaaagg cctggtgcat
     9721 atcgtcaaga tggtcaaacg cgaccactgg cgctttcagt aatgccgaac gcgttacgcc
     9781 gttagggaaa tatttttcgg caaagggttt tgagctgacg aacagatagt cgagcgcacc
     9841 aagtttatcg acaagacagc tcggcagcgc ctgatgttga atactcaccg cgccgaccac
     9901 ttcgccgcgg cgcagacgtt cctgagtgcg ggtttcatct tctacctgca agttgaggcg
     9961 gataggcgaa tcagccaaca caggagccag tgcaggaagc aaccacgtcg ccagactgtc
    10021 ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca
    10081 ctcttcttcc agcaactcca cctggcgcag cagtgccagc agtttttgcc cttgttccgt
    10141 cgggcgcggc ggtacggtac gcaccaacag cggctgcccg aacatatttt ccagttgctt
    10201 aatgcgctgt gagacggctg attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc
    10261 tcgttcacgt atcaccgcat ccagtgcctg taatgttctg tagtccgggc gtttcattgc
    10321 tgttgtctgc tcctggtaaa tgctttttcc tgcactatga cacaattttg tgtcaggttg
    10381 catataccga agccgtcggc gggtaacatt tgtattcgtg gttaaacgcg cagcttaaaa
    10441 gatcagggat ttgcgctaat gcatttcgct cagcgagttc gcgccctggt tgtgttgaat
    10501 ggcgtggcgt tattgcctct gttcgcaccg tggagcggca tacccagacc gttgtatgct
    10561 ttatttgcgg ggcgaaaggg gatgcccgcc attgcgcgat attttatgga tgagttaacc
    10621 acgcggcttg ccaacggggt ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca
    10681 taccacaggc gaaacgatca tgacgcagga tgaattgaaa aaagcagtag gatgggcggc
    10741 acttcagtat gttcagcccg gcaccattgt tggtgtaggt acaggttcca ccgccgcaca
    10801 ctttattgac gcgctcggta caatgaaagg ccagattgaa ggggccgttt ccagttcaga
    10861 tgcttccact gaaaaactga aaagcctcgg cattcacgtt tttgatctca acgaagtcga
    10921 cagccttggc atctacgttg atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa
    10981 aggcggcggc gcggcgctga cccgtgaaaa aatcattgct tcggttgcag aaaaatttat
    11041 ctgtattgca gacgcttcca agcaggttga tattctgggt aaattcccgc tgccagtaga
    11101 agttatcccg atggcacgta gtgcagtggc gcgtcagctg gtgaaactgg gcggtcgtcc
    11161 ggaataccgt cagggcgtgg tgaccgataa tggcaacgtg atcctcgacg tccacggcat
    11221 ggaaatcctt gacccgatag cgatggaaaa cgccataaat gcgattcctg gcgtggtgac
    11281 tgttggcttg tttgctaacc gtggcgcgga cgttgcgctg attggcacac ctgacggtgt
    11341 caaaaccatt gtgaaatgat ctgacggggg aacctccccc gttaaaaaaa ttctcttcat
    11401 taaatttggt gacatgtgtc acgcttttac caggcaattg tcgattgctc taaataaatc
    11461 ctctaaacca gcatattcat ccaagaatta cctttgcgtg atatttcctc aacatcgcga
    11521 cgcaaacgtt catattgccg caatattatt ttttgatatg ttgaaaggcg gatgcaaatc
    11581 cgcacacaac atttcaaaag acaggattgg gtaaatggca aaggtatcgc tggagaaaga
    11641 caagattaag tttctgctgg tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc
    11701 agctggttac accaacatcg aatttcacaa aggcgcgctg gatgatgaac aattaaaaga
    11761 atccatccgc gatgcccact tcatcggcct gcgatcccgt acccatctga ctgaagacgt
    11821 gatcaacgcc gcagaaaaac tggtcgctat tggctgtttc tgtatcggaa caaaccaggt
    11881 tgatctggat gcggcggcaa agcgcgggat cccggtattt aacgcaccgt tctcaaatac
    11941 gcgctctgtt gcggagctgg tgattggcga actgctgctg ctattgcgcg gcgtgccgga
    12001 agccaatgct aaagcgcacc gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc
    12061 gcgcggcaaa aagctgggta tcatcggcta cggtcatatt ggtacgcaat tgggcattct
    12121 ggctgaatcg ctgggaatgt atgtttactt ttatgatatt gaaaacaaac tgccgctggg
    12181 caacgccact caggtacagc atctttctga cctgctgaat atgagcgatg tggtgagtct
    12241 gcatgtacca gagaatccgt ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat
    12301 gaagcccggc tcgctgctga ttaatgcctc gcgcggtact gtggtggata ttccggcgct
    12361 gtgtgatgcg ctggcgagca aacatctggc gggggcggca atcgacgtat tcccgacgga
    12421 accggcgacc aatagcgatc catttacctc tccactgtgt gaattcgaca acgtccttct
    12481 gacgccacac attggcggtt cgactcagga agcgcaggag aatatcggcc tggaagttgc
    12541 gggtaaattg atcaagtatt ctgacaatgg ctcaacgctc tctgcggtga acttcccgga
    12601 agtctcgctg ccactgcatg gtggtcgtcg tctgatgcac atccacgaaa accgtccggg
    12661 cgtgctaact gcgctgaaca aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata
    12721 tctacaaact tccgcccaga tgggttatgt agttattgat attgaagctg acgaagacgt
    12781 tgccgaaaaa gcgctgcagg caatgaaagc tattccgggt accattcgcg cccgtctgct
    12841 gtactaattc cccttctctg aaaattaacg ggcaggtcac tgacttgccc gtttttttat
    12901 cccttctcca caccgttgcc gcgttgcgca ttctactttg cgagtctgct ccagaaaata
    12961 aatcaaagtc agatcaatgc gttgtgttta tttaatgggg atttacgatg gcgggacagc
    13021 atggggctgt aacggtaaag ccctcaccga agcgagggct taaaggagaa gggttatgat
    13081 gcgacttgtc atcatactga ttgtactgtt actgataagt ttcggagctt attaacagtc
    13141 agtctcaggg gaggagtcat cctcccttac ccttactcac taaattaggt caaagaatca
    13201 acgatgtcaa tcagggcgat gcggggtgca tcgccctgaa ccgaactttt tgatagccac
    13261 ttcatcgccc acgtttactg ccgtaataga tcctttttcc caaaaattat ctctgttttg
    13321 catggggttt ttcgagcacg ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt
    13381 tatttaaaga gagcttacga tagctgggca gcatggggct gtaacggtaa agccctcacc
    13441 gaagcgaggg cttgaaggag aagggttatg atgcgacttg ccatcatact gattgtactg
    13501 ttactcataa gtttcccagc ttattaacag tcagtcacag gggaggagca atcctccctt
    13561 acccttactc actaaattag gttaaagaat caacgatgtc aatcagggcg atgcaggtgt
    13621 atcgccctta ccactcccag actttcgacg gtgtaaccac cgcaggaaga gggatatccc
    13681 actcttcaac ggggagtttt tccaccaact gacaatcatg cgcataaccc accggttgcg
    13741 ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa accaccgccc attcccaggc
    13801 gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg
    13861 gtagcacgtc acgcacatcc agttttggct catggatctt caacctgttc atcaccagtt
    13921 cgctttgcgg atggtaattc aggaacagca aattaccggc actaaaggga tgcaaaactg
    13981 gaagatatac gcgcttaccg gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt
    14041 caccatcaaa agagaggaat acagcgaccg tatgcgccat caccaccggg ggataagtca
    14101 tcatccgggt agcggcttgt tgacccattt cctgctgttg ttccggcgtt aacgcacgac
    14161 gacgttgccg aatcattttg cggatttctt gtcgggataa tgtcagtggg agttctggta
    14221 gttgcgtcat ggctggtgcc agataagaag ggaatctccg agatgccgcc gcaggctgta
    14281 acccttgaac ccttggttca aggtgaatgt gtcgtcgcag ttttaaggct tctcggacgg
    14341 accgagcatg ctcaccaacc gcggagcgcc acattcttgt ggtatgaaat atcggctcag
    14401 gggactggcc cgcttgcgaa catctcagag aaattttgtc ttcacggtta ctctaccaca
    14461 gtaaaccgaa aagtgtcatt caaagttttg gttagttttt tcggtgatgc gaccttgttc
    14521 aagtaacgct tgttctatgg tctgctgcag catccgaata cgctgttcca tacttgccgc
    14581 gtagtcacga gtcttcgctt tttcttgcgc taactcatag ctgatattca atgcggcaat
    14641 gaagaccaac tgttcagtat ttgtgactct agtgcgttct ttcagatctt gcaaccgttg
    14701 gttcagatcg tccgctgcct gattcaacgc atccctttgg tcaggcgggc agttcacacg
    14761 cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca gacatgccac cttcctgctg
    14821 attgactgcg ctgccttcgt ctttagaccc atggtctgcg aaggggcgac actatagcta
    14881 ccctgatgag aagagacaag cccttttctg gtccaccagg gcccaaagtg gtagcatatc
    14941 atgaatattc ctccctttga cgacgaatgc ttatgtctat acagaacgaa atgcctggtt
    15001 acaacgaaat gaaccagtat ctgaaccaac aagggacggg tctgacccct gctgagatgc
    15061 atggtttaat cagtgggatg atatgtggcg gtaacgatga cagctcatgg ctaccgctac
    15121 ttcacgacct gacgaacgaa ggcatggctt tcggtcatga gctggcacag gcactgcgta
    15181 aaatgcactc tggcaccagc gatgccctgc aggatgacgg cttccttttt cagctttatc
    15241 tgcctgatgg cgatgatgtc agcgttttcg atcgggctga tgcattggca ggttgggtca
    15301 atcacttcct gcttggtctt ggcgttacgc aaccgaagct ggataaagtg accggcgaaa
    15361 ccggtgaagc tatcgacgat ctgcgtaaca ttgcgcaact gggttacgac gaagacgaag
    15421 atcaggaaga gcttgaaatg tcgcttgaag agatcatcga atacgttcgt gttgccgcgc
    15481 tgttatgcca cgacaccttt actcatccgc aaccgaccgc gccagaagta caaaaaccga
    15541 ctctacacta aaaacaaaaa acgtaaggag agtgttatga gtgagatatc ccggcaagag
    15601 tttcagcgtc gccgtcaggc cctggtggag caaatgcaac ccggcagcgc cgcgctgatt
    15661 tttgctgcac cagaagtaac acgtagcgcc gacagcgaat acccctatcg tcagaacagt
    15721 gacttctggt acttcaccgg ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc
    15781 gatgacactc ataaccacag cgttctgttt aaccgcgttc gcgacctgac ggcggagatc
    15841 tggtttggcc gtcgcttagg ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca
    15901 ctggcattca gcgaaatcaa tcagcaactt tatcaactac ttaacggcct ggatgtggtt
    15961 taccatgccc agggcgaata tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa
    16021 ctgcgtaaag gttcgcggca aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct
    16081 gttgttcatg aaatgcgcct gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg
    16141 ggagaaatca ccgccctggc acatacccgg gcgatggaaa aatgccgtcc gggaatgttc
    16201 gagtaccatc tggaaggcga aattcaccac gaatttaacc gccacggtgc gcgctatcct
    16261 tcctataaca ccattgtcgg cagcggtgaa aacggctgca ttttgcacta caccgaaaac
    16321 gagtgtgaac tgcgtgacgg tgacctggtg ttgattgacg cgggttgtga atacaaaggt
    16381 tacgctggcg atattacccg caccttcccg gtcaacggca aattcactca ggcccagcgt
    16441 gaaatctacg acattgtgct ggagtctctc gaaaccagct tgcgcctgta tcgtccggga
    16501 acttccatgc aggaagtcac cggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa
    16561 ctcggcattc tgaaaggtga tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc
    16621 tttatgcatg gccttagcca ctggttaggg ctagatgtcc atgacgttgg cgtttatggt
    16681 caggatcgct cgcgcattct ggaaccgggg atggtactga ccgtagagcc tggtctgtat
    16741 atcgcgccgg atgcggatgt gccagaacaa tatcgcggta tcggcattcg tattgaagac
    16801 gacattgtga ttaccgaaac cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg
    16861 gaagaaatcg aagcgttgat ggcagctgcg agaaagcaat gagcgtaatc atcgtcggtg
    16921 gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg gttaagtcac ggggcgttgc
    16981 cggtacattt gattgaagcg actgcgccag agtcacatgc tcatccgggc tttgatggac
    17041 gagcgatagc gctggcggcg ggtacctgtc agcaactggc gcgcatcggc gtttggcaat
    17101 ctctggcgga ttgcgcaact gccatcacca ccgtgcatgt cagcgatcgt ggtcacgctg
    17161 gatttgtcac cctcgccgca gaagattacc aactggcggc gctgggacag gttgtcgaat
    17221 tgcacaatgt cgggcaacgg ctgtttgcat tgctgcgtaa agcacctggc gtaacgctgc
    17281 attgccctga tcgcgtggct aacgttgccc gtactcagag tcacgttgaa gtgacgctgg
    17341 agagtggcga gacgctgacg ggccgcgtgc tggtagccgc tgatggcacc cattcagcgt
    17401 tagccaccgc gtgcggcgtt gactggcagc aggagcctta cgaacaactg gcagtgattg
    17461 ccaacgttgc tacttctgtt gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg
    17521 gcccgctggc gatgttgccg atgtctgacg gacgctgttc gctggtctgg tgtcatccac
    17581 tggaacggcg cgaagagctg ctgtcgtgga gtgacgagaa gttttgccgt gaactccagt
    17641 cggcctttgg ctggcgactt ggaaaaatta cccacgccgg taaacgcagt gcttatccgc
    17701 tggcgttaac ccgcgccgcc agagctatta cccatcgtac cgtgctggtg ggcaatgcgg
    17761 cgcaaactct gcacccgatt gccgggcaag ggtttaacct cggtatgcga gatgtcatga
    17821 gccttgcgga aaccctgact caggcgcatg agcgcggaga agacatcggt gattacggca
    17881 tattgtgccg ctatcagcag cgtcgacaga gcgatcgcga agcaaccatt ggcgtcacgg
    17941 acagccttgt acatcttttt gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg
    18001 gactgatgac gatggaatta ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg
    18061 gttgggtggc gcgttgaagg cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc
    18121 attgttggcg gcggcatggt ggggctggcg gttgcctgcg gcttacaggg gagcggctta
    18181 cgcgttgccg tactggagca gcgcgtaccg gaacctctgg cggcggatgc accaccacaa
    18241 ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac tcacccgtct tggcgtctgg
    18301 caggacattc tctctcgtag ggctagttgt tatcacggta tggaagtgtg ggacaaagac
    18361 agttttggtc acatttcgtt tgacgatcaa agcatgggct atagccatct tgggcatatc
    18421 gttgaaaact cagtgattca ctacgcgctg tggagcaaag cgcagcaggc gtcagatatc
    18481 accctgttgg cccccgcaga attacagcag gtcgcctggg gagaaaatga aactttcctg
    18541 acgctgaaag atggcagcat gttaacggcg cgtctggtga ttggcgcgga cggcgctaat
    18601 tcctggttgc gcaacaaagc agatattccg ctgactttct gggattatca gcatcacgcg
    18661 ctggtagcga ccattcgcac ggaagaaccg catgatgcgg tggcgcggca ggttttccat
    18721 ggcgaaggca ttctggcctt tttaccgctt agcgatccgc atctttgctc gattgtctgg
    18781 tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa gtgaagacga atttaatcgc
    18841 gcgttaaata tcgcttttga taatcgcctg ggcttatgca aggttgagag cgcgcgtcag
    18901 gtgttcccac tgacggggcg ttatgcgcac cagtttgccg cgcaccgtct ggcgctggtg
    18961 ggcgacgccg cgcataccat tcacccgctg gcggggcagg gggtaaatct tggctttatg
    19021 gatgctgcag agctgattgc tgaactgaaa cggttgcatc gtcagggtaa agacatcggg
    19081 cagtacattt atctgcgtcg ctatgagcgt agccgcaagc acagttcggc gctgatgctg
    19141 gctggtatgc agggattccg cgatctgttt tccggtacca atccggcgaa aaaactgctg
    19201 cgtgatattg gtttgaaact ggccgacacg cttcctggcg ttaaaccaca actcatccgc
    19261 caggcaatgg gattaaacga tttgcctgaa tggctgcgtt aaaaatttct cttctgttgt
    19321 ttatttgata cccatcacac tttcatctcc cggttttttc gccgggagat tttcctcatt
    19381 tgaaataaac taatttcacc tccgttttcg cattatattt tctaatgcca tgattttttg
    19441 atttagtgtt ttttgacatt tttttagttc ttaatattgc cttattcaaa ttaactttct
    19501 aatggcatca tctttatata gaaactagtg tgttttttgg ttttttattc tgtcgcgatt
    19561 ttggcgtttt ttaaccataa gctaatgtga tgatcaattt taccttatgg ttaacagtct
    19621 gtttcggtgg taagttcagg caaaagagaa cgattgcgtt ggggaccggg agtggctccg
    19681 atgctgggtt tcgtggtgat aatttcacca tgaaaagttg tcagccccgc ttattcaatg
    19741 aggacaagat ggcacaacag actcctttgt acgaacaaca cacgctttgc ggcgctcgca
    19801 tggtggattt ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc
    19861 atgcggtacg taccgatgcc ggaatgtttg atgtgtcaca tatgaccatc gtcgatctcc
    19921 gcggcagccg cacccgggag tttctgcgtt atctgctggc gaacgatgtg gcgaagctca
    19981 ccaaaagcgg caaagccctt tactcgggga tgttgaatgc ctctggcggt gtgatagatg
    20041 acctcatcgt ctactacttt actgaagatt tcttccgcct cgttgttaac tccgccaccc
    20101 gcgaaaaaga cctctcctgg attacccaac acgctgaacc tttcggcatc gaaattaccg
    20161 ttcgtgatga cctttccatg attgccgtgc aagggccgaa tgcgcaggca aaagctgcca
    20221 cactgtttaa tgacgcccag cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc
    20281 aggcgggcga tctgtttatt gccaccactg gttataccgg tgaagcgggc tatgaaattg
    20341 cgctgcccaa tgaaaaagcg gccgatttct ggcgtgcgct ggtggaagcg ggtgttaagc
    20401 catgtggctt gggcgcgcgt gacacgctgc gtctggaagc gggcatgaat ctttatggtc
    20461 aggagatgga cgaaactatt tctcctttag ccgccaacat gggctggact atcgcctggg
    20521 aaccggcaga tcgtgacttt atcggtcgtg aagccctgga agtgcagcgt gagcatggta
    20581 cagaaaaact ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg
    20641 tacgctttac cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc ggtactttct
    20701 ccccgacgct gggttacagc attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg
    20761 cgattgtgca aattcgcaac cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc
    20821 gtaacggcaa agccgtcgcg tgatttactt ttttggagat tgattgatga gcaacgtacc
    20881 agcagaactg aaatacagca aagaacacga atggctgcgt aaagaagccg acggcactta
    20941 caccgttggt attaccgaac atgctcagga gctgttaggc gatatggtgt ttgttgacct
    21001 gccggaagtg ggcgcaacgg ttagcgcggg cgatgactgc gcggttgccg aatcagtaaa
    21061 agcggcgtca gacatttatg cgccagtaag cggtgaaatc gtggcggtaa acgacgcact
    21121 gagcgattcc ccggaactgg tgaacagcga accgtatgca ggtggctgga tctttaaaat
    21181 caaagccagc gatgaaagcg aactggaatc actgctggat gcgaccgcat acgaagcatt
    21241 gttagaagac gagtaacggc tttattcctc ttctgcggga gaggatcagg gtgaggaaaa
    21301 tttatgcctc accctcactc tcttcgtaag gagagaggtt cacaattcac tgcacgtttc
    21361 aggaaccatc gctcatgaca cagacgttaa gccagcttga aaacagcggc gcttttattg
    21421 aacgccatat cggaccggac gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac
    21481 aatcgttaaa cgcgctgacc ggccagattg tgccgaaaga tattcagctt gcgaccccgc
    21541 cgcaggttgg cgcaccggcg accgaatacg ccgcactggc agaactcaag gctattgcca
    21601 gtcgcaataa acgcttcacg tcttacatcg gcatgggtta caccgccgtg cagctaccgc
    21661 cggttatcct gcgtaacatg ctggaaaatc cgggctggta taccgcgtac actccgtatc
    21721 aacctgaagt ctcccagggc cgccttgaag cactgctcaa cttccagcag gtaacgctgg
    21781 atttgactgg actggatatg gcctctgctt ctcttctgga cgaggccacc gctgccgccg
    21841 aagcaatggc gatggcgaaa cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg
    21901 cttccgatgt gcatccgcaa acgctggatg tggtccgtac tcgtgccgaa acctttggtt
    21961 ttgaagtgat tgtcgatgac gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc
    22021 tgttacagca ggtaggcact accggtgaaa ttcacgacta cactgcgctt attagcgaac
    22081 tgaaatcacg caaaattgtg gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa
    22141 ctgcgccggg taaacagggc gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc
    22201 cgatgggcta cggtggccca cacgcggcat tctttgcggc gaaagatgaa tacaaacgct
    22261 caatgccggg ccgtattatc ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca
    22321 tggcgatgca gactcgcgag caacatatcc gccgtgagaa agcgaactcc aacatttgta
    22381 cttcccaggt actgctggca aacatcgcca gcctgtatgc cgtttatcac ggcccggttg
    22441 gcctgaaacg tatcgctaac cgcattcacc gtttgaccga tatcctggcg gcaggcctgc
    22501 aacaaaaagg tctgaagctg cgccatgcgc actatttcga cactctgtgt gtggaagtgg
    22561 ccgacaaggc gggcgtactg gcgcgtgccg aagcggctga aatcaacctg cgtagcgata
    22621 tcctgaacgc ggttgggatc acccttgatg aaaccaccac gcgtgaaaac gtaatgcagc
    22681 ttttcagcgt gctgctgggc gataaccacg ggctggagat cgacacgctg gacaaagacg
    22741 tggctcacga cagccgctct attcagcctg cgatgctgcg cgacgacgaa atcctcactc
    22801 atccggtgtt taatcgctac cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg
    22861 agcgtaaaga tctggcgctg aatcaggcga tgatcccgct gggttcctgc accatgaaac
    22921 tgaacgccgc cgccgagatg atcccaatca cctggccgga atttgccgaa ctgcacccgt
    22981 tttgcccgcc ggaacaggct gaaggttatc agcagatgat tgcgcagctg gctgactggc
    23041 tggtgaaact gaccggttac gacgccgttt gtatgcagcc gaactccggc gcacagggcg
    23101 aatacgcggg cctgctggcg attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg
    23161 atatctgcct gatcccggct tctgcgcacg gaactaaccc cgcttctgca catatggcag
    23221 gaatgcaggt ggtggttgtg gcgtgtgata aaaacggcaa catcgatctg actgatctgc
    23281 gcgcgaaagc ggaacaggcg ggcgataacc tctcctgtat catggtgact tatccttcta
    23341 cccacggcgt gtatgaagaa acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg
    23401 gtcaggttta ccttgatggc gcgaacatga acgcccaggt tggcatcacc tcgccgggct
    23461 ttattggcgc ggacgtttcg cacctcaacc tgcataaaac tttctgcatt ccgcacggcg
    23521 gtggtggtcc gggtatggga ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg
    23581 gtcatagcgt ggtgcaaatc gaaggcatgt taacccgtca gggcgcagtt tctgcggcac
    23641 cgttcggtag cgcctctatc ctgccaatca gctggatgta catccgcatg atgggcgcag
    23701 aagggctgaa aaaagcaagc caggtggcaa tcctcaacgc caactatatt gccagccgcc
    23761 tgcaggatgc cttcccggtg ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta
    23821 ttctcgatat tcgcccgctg aaagaagaaa ccggcatcag cgagctggat attgccaagc
    23881 gcctgatcga ctacggtttc cacgcgccaa cgatgtcgtt cccggtggcg ggtacgctga
    23941 tggttgaacc gactgaatct gaaagcaaag tggaactgga tcgctttatc gacgcgatgc
    24001 tggctatccg cgcagaaatt gaccaggtga aagccggtgt ctggccgctg gaagataacc
    24061 cgctggtgaa cgcgccgcac attcagagcg aactggtcgc cgagtgggcg catccgtaca
    24121 gccgtgaagt tgcggtattc ccggcaggcg tggcagacaa atactggccg acggtgaaac
    24181 gtctggatga tgtttacggc gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat
    24241 accagtaatt cactgattcg actattttct aaaggcgctt cggcgccttt ttagtcagat
    24301 gacaaagtac aaaagtgctc agacagtccc ctcgcccctt tggggagagg gtgaggggaa
    24361 caggccggca ctggcgcgaa tatttaccct caccccggcc ctctccctga aagggcgagg
    24421 gggaaaagcg tgccaacatt gaagattgag ccagtttgtt agcaatctca aagatacgtc
    24481 aacgaatgaa tttttctcgg aaaaacaaat ggctatagca cttgtgaccg gtggcagtcg
    24541 cggcatcggg cgggcaactg cattactgtt ggcgcaagaa gggtatacgg tggcggttaa
    24601 ttatcaacaa aacctccacg cggcgcagga agtgatgaac ttaataacgc aagccggtgg
    24661 caaagcattc gtgctcca
//
LOCUS       DAFATA010000062        24544 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFATA010000062 DAFATA010000000
VERSION     DAFATA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.063"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..532)
                     /gene="yiaJ"
                     /locus_tag="LGJ20_003008"
     CDS             complement(<1..532)
                     /gene="yiaJ"
                     /locus_tag="LGJ20_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBK2387038.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWE"
     gene            732..1730
                     /gene="yiaK"
                     /locus_tag="LGJ20_003009"
     CDS             732..1730
                     /gene="yiaK"
                     /locus_tag="LGJ20_003009"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBK2387039.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1742..2209
                     /locus_tag="LGJ20_003010"
     CDS             1742..2209
                     /locus_tag="LGJ20_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBK2387040.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            2327..2800
                     /gene="yiaM"
                     /locus_tag="LGJ20_003011"
     CDS             2327..2800
                     /gene="yiaM"
                     /locus_tag="LGJ20_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HBK2387041.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            2803..4080
                     /gene="yiaN"
                     /locus_tag="LGJ20_003012"
     CDS             2803..4080
                     /gene="yiaN"
                     /locus_tag="LGJ20_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBK2387042.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            4093..5079
                     /gene="yiaO"
                     /locus_tag="LGJ20_003013"
     CDS             4093..5079
                     /gene="yiaO"
                     /locus_tag="LGJ20_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBK2387043.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            5083..6579
                     /gene="lyxK"
                     /locus_tag="LGJ20_003014"
     CDS             5083..6579
                     /gene="lyxK"
                     /locus_tag="LGJ20_003014"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBK2387044.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLIHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            6576..7238
                     /gene="ulaD"
                     /locus_tag="LGJ20_003015"
     CDS             6576..7238
                     /gene="ulaD"
                     /locus_tag="LGJ20_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK2387045.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVMQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            7231..8091
                     /locus_tag="LGJ20_003016"
     CDS             7231..8091
                     /locus_tag="LGJ20_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK2387046.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            8085..8780
                     /gene="araD"
                     /locus_tag="LGJ20_003017"
     CDS             8085..8780
                     /gene="araD"
                     /locus_tag="LGJ20_003017"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK2387047.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(8812..9627)
                     /locus_tag="LGJ20_003018"
     CDS             complement(8812..9627)
                     /locus_tag="LGJ20_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2387048.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            9853..11253
                     /locus_tag="LGJ20_003019"
     CDS             9853..11253
                     /locus_tag="LGJ20_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387049.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKITWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            11264..13243
                     /locus_tag="LGJ20_003020"
     CDS             11264..13243
                     /locus_tag="LGJ20_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBK2387050.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVISYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGV
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(13364..14104)
                     /locus_tag="LGJ20_003021"
     CDS             complement(13364..14104)
                     /locus_tag="LGJ20_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HBK2387051.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFVTGVSWRF"
     gene            14228..15202
                     /locus_tag="LGJ20_003022"
     CDS             14228..15202
                     /locus_tag="LGJ20_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2387052.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(15199..16335)
                     /locus_tag="LGJ20_003023"
     CDS             complement(15199..16335)
                     /locus_tag="LGJ20_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2387053.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(16341..16664)
                     /locus_tag="LGJ20_003024"
     CDS             complement(16341..16664)
                     /locus_tag="LGJ20_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBK2387054.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            16835..16990
                     /locus_tag="LGJ20_003025"
     CDS             16835..16990
                     /locus_tag="LGJ20_003025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387055.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            complement(17209..18747)
                     /gene="aldB"
                     /locus_tag="LGJ20_003026"
     CDS             complement(17209..18747)
                     /gene="aldB"
                     /locus_tag="LGJ20_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBK2387056.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(18912..20063)
                     /gene="yiaY"
                     /locus_tag="LGJ20_003027"
     CDS             complement(18912..20063)
                     /gene="yiaY"
                     /locus_tag="LGJ20_003027"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBK2387057.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(20253..22097)
                     /gene="selB"
                     /locus_tag="LGJ20_003028"
     CDS             complement(20253..22097)
                     /gene="selB"
                     /locus_tag="LGJ20_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBK2387058.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(22094..23485)
                     /gene="selA"
                     /locus_tag="LGJ20_003029"
     CDS             complement(22094..23485)
                     /gene="selA"
                     /locus_tag="LGJ20_003029"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBK2387059.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(23583..24191)
                     /locus_tag="LGJ20_003030"
     CDS             complement(23583..24191)
                     /locus_tag="LGJ20_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBK2387060.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
BASE COUNT         5824 a         6555 c         6253 g         5912 t
ORIGIN      
        1 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
       61 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
      121 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
      181 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
      241 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
      301 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
      361 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
      421 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
      481 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
      541 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
      601 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
      661 atatttgaaa tcaagtttcg catattgaaa ttttaagcca aaaagcgatc aaaaaaacaa
      721 ggaagcctgg gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
      781 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
      841 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
      901 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
      961 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
     1021 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
     1081 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
     1141 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc attggcacta
     1201 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
     1261 tgttctctta cggcatgtta gaagttaacc gcctggcggg ccgtcagctc ccggtcgatg
     1321 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
     1381 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
     1441 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
     1501 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
     1561 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
     1621 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
     1681 acggtattac cgttgatgac agcgtgtggg ccaaaatcca ggcgttataa ggagataagt
     1741 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
     1801 ggggctcgat tttctgcgcg ctaccgattt caacgccctg gagccgggcg ttgtcgaaat
     1861 cgacggcaaa aatatttatg cgcaaattat cgatttaacc acacgcgaag cggtggaaaa
     1921 ccgtccggaa gtgcatcgtc ggtatatcga tattcagttt ctggcgtggg gtgaagagaa
     1981 aatcggcatt gctattgata cgggaaataa taaagtcagc gaatcactat tagagcagcg
     2041 cgatattatt ttttatcatg acagtgaaca tgaatcattt atagaaatga taccgggcag
     2101 ctacgcaatt ttctttccgc aggatgttca ccgaccaggt tgtattttgc aaacagcctc
     2161 tgagatacga aaaattgtgg ttaaagtcgc attaacggcg ctgaattaat cgaataatcg
     2221 tctacgccag aacgactggg taatgttatt gctctaacta tattaataat ttaggttgct
     2281 gcacatgcaa cttgaattat gacagacata gcctcaagga atagctatga aaaaaatact
     2341 tgaagcaata ctggcgatta atctcgccgt actttcctgt attgtgttta taaacattat
     2401 tttaagatat ggttttcaga caagcatttt atctgtagat gaattgtcac gttatttatt
     2461 tgtctggtta acatttattg gcgcgattgt ggcttttatg gataacgccc acgttcaggt
     2521 cacctttctg gtggagaaac tctcccccgc atggcaacga cgagtcgctc tggttaccca
     2581 ttccttaatc ttatttattt gtggcgcact ggcctggggc gcaacgctaa aaacaattca
     2641 ggactggagc gattattcac cgatcctcgg tttacccatc ggcctgatgt atgccgcttg
     2701 tctgcccacc agcctcgtta tcgcattctt tgaactgcgt catttatatc aactcatcac
     2761 gcgtagcaat tctctaacgt cgccaccgca aggagcttaa tcatggctgt gctgattttt
     2821 ctgggctgtc tgttgggtgg tattgctatc ggtttgccta ttgcctgggc actgttgttg
     2881 tgcggggcgg cgttaatgtt ctggctggac atgtttgatg tccagatcat ggcacaaacg
     2941 ctggtgaacg gagccgatag cttctccctg ctggcgattc cgttctttgt gctggcgggt
     3001 gaaatcatga atgcgggcgg cttgtcaaaa cgcattgttg acctgccgat gaaactggtg
     3061 gggcataaac caggcgggct gggctacgtc ggcgtgctgg cagctatgat tatggcgagc
     3121 ctttctggtt ctgccgttgc ggataccgcc gccgttgccg ctctactggt gccgatgatg
     3181 cgcagcgcca actacccggt caaccgggcg gcggggctga ttgcctctgg cggcattatc
     3241 gcgccaatta ttcccccttc cattccgttt attatcttcg gcgtttccag cggattatcc
     3301 atcagtaagc tgtttatggc gggcattgcc cctggcatga tgatgggcgc aacgctgatg
     3361 cttacctggt ggtggcaggc cagtcgcctt aatttacccc gccagcaaaa agcaacgatg
     3421 caggaaatct ggcactcttt tgtctccggt atctgggcgc tgtttcttcc agtcatcatt
     3481 attggcgggt ttcgctccgg tctgtttacc cctaccgaag ccggggccgt tgctgctttc
     3541 tatgcgctgt ttgtcgccac agttatttac cgtgaaatga ccttcgccac actttggcat
     3601 gtactcattg gcgcggcgaa aaccacctca gtggttatgt ttctggtggc ctcggcacaa
     3661 gtttccgcct ggctgattac cattgctgaa ctgccgatga tggtttctga tttactgcaa
     3721 ccgttggtcg attcaccgcg tctgctgttt atcgtcatta tggtggctat cctgattgtc
     3781 ggcatggtca tggatttaac gccgacggta ttaattctta ccccggtatt aatgccttta
     3841 gttaaagagg caggaatcga tccgatttat ttcggtgtca tgtttatcat taactgctca
     3901 atcggcttaa ttacaccgcc tatcggtaat gtacttaacg ttatttcagg ggtggcaaaa
     3961 ctcaaattcg atgatgcagt cagaggcgtt ttcccttacg tcctggtttt atattcatta
     4021 ttagtcgtat ttgtttttat tcccgatctc atcatcctcc ctttgaaatg gattaattaa
     4081 aaggaaaata ttatgaaatt acgctctgta acctacgcat tattcattgc tggcctggct
     4141 gcattcagca catcttctct ggcggcgcaa tctttacgtt tcggttatga aacctcacaa
     4201 accgactcgc aacatattgc ggcgaaaaaa ttcaatgatt tattgcagga gagaaccaaa
     4261 ggcgagctga aattaaaatt gtttccggat agcaccctcg gtaacgcgca ggcgatgatc
     4321 agcggcgtac gtggcggcac catcgatatg gaaatgtccg gctcgaataa ctttgccggg
     4381 ttatcaccag tgatgaactt gcttgatgtc cctttcctgt tccgcgatac cgctcacgcg
     4441 cataaaacgc tcgacggcaa agtcggtgat gatctgaaag cctcacttga aggtaaagga
     4501 ctaaaagtac tggcctactg ggaaaacggc tggcgcgatg tcaccaactc gcgcgcaccg
     4561 gttaaaaccc ccgccgacct gaaagggctg aaaattcgca ccaacaatag cccgatgaat
     4621 atcgccgcat tcaaagtctt tggcgctaac ccgatcccga tgccgtttgc cgaagtctat
     4681 accgggctgg aaacccgcac tatcgacgct caggaacacc cgatcaacgt cgtctggtca
     4741 gcaaaatttt tcgaagtgca gaagtacctt tctctgacgc accacgccta ttccccgctt
     4801 ctggtggtga tcaacaaagc gaagtttgat ggcttaagcc cggagttcca gcaagcgcta
     4861 atttcatctg cgcaggaagc gggtaactac cagcgcaaac tggttgccga agatcagcaa
     4921 aaaatcatcg acggcatgaa agaagcgggc gtggaagtca tcaccgatct cgaccgcaaa
     4981 gcctttagcg acgcactggg tactcaggtc cgcgacatgt ttgtaaaaga tgttccgcag
     5041 ggtgctgatc tactgaaagc cgtggatgag gtgcaataaa ccatgacgca atactggctg
     5101 gggttagatt gtggcggtag ctggctgaaa gccgggctgt atgaccgcga aggccgggag
     5161 gcaggcgtgc agcgcctgcc gctgtgcgca ttaagcccgc agccaggttg ggcagagcgc
     5221 gatatggcag aactgtggca atgctgcatg gctgtcattc gcgaactgct tatccattcc
     5281 ggcgttagcg gggaacaaat tgtcggtatc ggtatctccg cacaaggaaa aggcttgttt
     5341 ttgctggata aaaacaacaa gccgctcggt aacgccattt tgtcctcgga tcgccgggcg
     5401 atggaaatcg ttcgtcgctg gcaggaagat ggcatcccgg aaaaactcta cccgttgacc
     5461 cgacaaacct tgtggaccgg gcatccggtg tcgctgttac gctggctgaa agagcacgaa
     5521 ccggaacgct acgcgcaaat tggctgcgtg atgatgacgc acgactacct gcgctggtgt
     5581 ttaaccggcg tcaaaggctg tgaagagagc aatatttccg agtccaacct ctacaacatg
     5641 agtcttgggg aatatgaccc gtgcctcacc gactggctgg ggattgctga aatcaatcac
     5701 gccctgccgc ctgttgtcgg atctgccgaa atctgcgggg agatcaccgc tcagacagcc
     5761 gtactgaccg gtctgaaagc gggtacgccc gttgtcggcg gcctgtttga tgtggtttcc
     5821 accgcactct gcgccgggat cgaagacgaa tttaccctta atgcggtgat gggcacctgg
     5881 gcggtgacca gcggcataac ccgcggttta cgtgacggtg aagcgcatcc gtatgtctat
     5941 ggtcgctacg ttaacgatgg tgaatttatc gttcacgaag ccagccccac ctcttccggc
     6001 aacctcgaat ggtttaccgc acagtgggga gaaatctcgt ttgatgagat caaccaggcc
     6061 gttgccagct tgccgaaggc cggtggcgat ctctttttcc tgccgttcct gtacggcagc
     6121 aacgccggac tcgagatgac cagcggtttc tacgggatgc aggccattca cacccgcgca
     6181 cacctgttgc aggccattta tgaaggggtg gtgttcagcc atatgaccca cctcaaccgg
     6241 atgcgcgaac gttttactga tgtgcacacc ctgcgcgtca ctggcggccc ggcgcactcc
     6301 gatgtctgga tgcaaatgct ggcggacgtc agcggtctgc gtatcgagct gccgcaggtg
     6361 gaagaaaccg gctgttttgg cgcggcactt gccgcccgtg tcggcaccgg agtttatcgc
     6421 gatttcagcg aagctcaacg tgacttacag cacccggtac gcaccctgct gccggatatg
     6481 accgcccatc agctttacca acaaaaatac caacgctatc agcatctcat tgccgcactt
     6541 cagggctttc acgcccgcat caaggagcac atattatgag ccgaccactt ctgcaactgg
     6601 cactcgacca ctcatcactt gaagccgcac agcgcgacgt gatgcagtta aaagacagcg
     6661 tcgatattgt cgaagcgggc accattctct gtttaaacga agggcttggc gcggtgaaag
     6721 ccttgcgcga acagtgccct gacaaaatca tcgttgccga ctggaaggtc gccgacgctg
     6781 gtgaaacgct cgcgcaacag gcatttggcg caggcgctaa ctggatgacc atcatctgcg
     6841 ccgcaccgct cgcgacggtg gaaaaaggcc acgcgatggc gcaacgctgc ggtggtgaaa
     6901 ttcagatcga gctgttcggt aactggacgc tggacgacgc ccgcgactgg catcgtattg
     6961 gcgtgcggca ggcgatttat catcgcggtc gtgatgcaca ggccagcggg caacagtggg
     7021 gcgaagccga tctggcacgc atgaaggcgc tttccgatat cggccttgag ctttccatta
     7081 ccggcgggat tacccctgct gacctgccgc tgtttaaaga tattcgcgtg aaagcgttta
     7141 ttgccgggcg agcactggca ggctcagcaa atccggcgca ggtggctggc gatttccatg
     7201 cgcaaatcga cgctatctgg ggaggcaagc gtgcgtaatc atccgttagg gatttatgaa
     7261 aaggcgctgg cgaaagatct ctcctggccg gagcggctgg tgctggcaaa aagctgcggt
     7321 tttgattttg tcgaaatgtc ggtggatgaa accgacgaac ggctttcacg tcttgactgg
     7381 agcaccgcac aaaggacttc gctggttgcc gcgatgatcg aaacgggtgt tgggatcccg
     7441 tcgatgtgcc tgtccgcaca tcgtcgcttt ccttttggta gccgtgacga cgcggtacgc
     7501 cagcgagcac gggagatcat gagcaaagcg attcgtctgg cacgcgatct cggcatacgc
     7561 accatccagc tggcgggcta cgacgtctat tacgaagacc acgatgaagg cacccggcaa
     7621 cgttttgctg aagggctggc gtgggcggtc gaacaggcgg cggcatcaca agtcatgcta
     7681 gcggtggaga ttatggacac cgcgtttatg aactccatca gtaaatggaa aaagtgggac
     7741 gagatgctcg cctcgccgtg gttcaccgtc tacccggacg ttggcaacct cagcgcctgg
     7801 ggcaatgatg ttcccgccga actgaaactg ggcattgacc gtatcgccgc gatccacctg
     7861 aaagacaccc agccagttac cgagcaaagt cccggacagt tccgcgacgt gccgtttggc
     7921 gaaggctgcg tcgatttcgt tggcatcttc aaaacgctgc ataaactgaa ctatcgcggt
     7981 tctttcctga ttgagatgtg gaccgaaaaa gccaaagagc cggtgctgga gattattcag
     8041 gcgcggcgtt ggattgaagc gcgtatgcag gaggctggat ttatatgtta gagcaactga
     8101 aagccgacgt gctggcggcg aatctggcgc ttcccgctca ccatctggtg acgttcacct
     8161 ggggcaatgt cagcgcggta gacgaaacgc ggcaatggat ggtaatcaaa ccttccggcg
     8221 tcgagtatga cgtgatgacc gccgacgata tggtggtggt tgagatagcc agcggcaagg
     8281 tggtggaagg tagcaaaaag ccctcgtccg acacgccaac gcatctggcg ctttaccgtc
     8341 gctacgccga aatcggtggc attgtgcata cccactcacg ccacgccacc atctggtcgc
     8401 aggccgggct ggatctcccc gcctggggca ccacgcacgc tgattatttc tatggtgcca
     8461 tcccctgcac gcgacagatg accgcagagg agattaacgg cgaatatgaa tatcagaccg
     8521 gcgaagtgat cattgaaacc ttcgaagaac gtggcaggag tccggcacaa atcccggcgg
     8581 tgctggtgca ttctcatggc ccgttcgcat ggggtaaaaa cgccgccgat gccgtgcata
     8641 acgccgtagt actcgaagaa tgcgcctata tgggcctatt ctcgcgccag cttgcgccgc
     8701 agctccctgc gatgcaaaac gaactgctgg ataagcacta cctgcgtaag catggggcca
     8761 atgcctatta cgggcagtaa tccctcacgc cggggcttca tcgccccggc actacgaatt
     8821 gatatgttcc ttgctgtaac gccgcttcca cgctgctggc gttaaaccgg tgtgcttgcg
     8881 aaaaatctgc cggaaatagc caacgtcatt aaacccgcac aacgcagcca cttcagtgag
     8941 cgatcgcgct tcgctaatta acagcttttc ggcttcgcgc actcgctggc gatggattgc
     9001 ttctgttaac gtcagatgaa acacccgccg gtagacgcgc ccaagataat cggcattgca
     9061 gtacagctcg cgggctaaaa gcgccgtgga aagcgggcga tgatattgcg tgcggatcag
     9121 tttctgcgct ttccaggcaa gggtggtgcc tgcgccatcg gcctgttcat caagacgcgc
     9181 gtcacgggtg agctgctgca aaatcagcag cacaataaac tccagcgccg gagtgcgttg
     9241 atgcttttcc tgttcgctta agaactggcg aaacagggcc accagcgcct gcggatcgac
     9301 aaccttcgtt tgttgcggca cgctaagatg agtaagccag cgagggtcgc tatccggcgc
     9361 tttaacctca aaatgcagcc agtaaaattt gagatcggcg ggaaaatcct ccaccccaac
     9421 atgttgtcga ttcggccaga gtaataaact ctctcccgca tcgaccagaa atatccgttc
     9481 ttcttcctga atcgttaatc gacctttttc gacaaagata atttcccatg actccagccg
     9541 ccttgcagga tgcctcccta cgccgcggga aataaataat ccaccatttt gcactttaat
     9601 cggtaacgtc atggataatt cgagcattga tatgtaattt cccgcttatt cattaatgat
     9661 taaacacatt accttttgat tattaatcaa aaacattaat ttcacctgat ccatttcaca
     9721 cttttaaatt caggtcggaa tcgtcacctt tttttactct tttctcacct tgttgatatt
     9781 cccgttcacg gcagaattaa tcaggtcatt gcaataaaac taaaaattgc ttcctattca
     9841 cgaggaatat ctatgacctc tacaccgatt actaccgctg atatcgctaa aggtaaaatt
     9901 gacgatgcgt tatctgtacg ggaaaaaata ggctacggcc tgggtgacgc tggcggcacc
     9961 gtaataactt gcctgatcat gaactttctc acctttttct acaccgacgt ttttggatta
    10021 actccggcgc tggttggcac gctgtttatt gcactgcgcg tgtttgatgc catctccgac
    10081 ccggtgatgg gcgtcattgc cgaccggacg caaagtcgct gggggcgctt tcgtccgtgg
    10141 cagttatgga ttgccattcc catcggcatt atcggcatcc tgacgttcac cgtgccagat
    10201 gccagcatgg gagtaaaaat cacctgggcg ttcggcactt acctgctcct ttcagtcggt
    10261 tataccgcca tcaacgtacc gtactgcgcg ctgatcaaca ccatgaccac ccgccacaat
    10321 gaagtgatct cctgccagtc ctggcgattc gttctctgcg gcgtagcggg atttctggtt
    10381 tcggtaggct taccgtggat ggtagatctc ttcggtcagg gcaacgctgc acgcggctat
    10441 caactgggcg tcggcgtatt gtgcgccatt gccgtggtga tgttcctgtg ctgttttttc
    10501 tgggttcgtg aacgggtgcc gctctccaca atggggaaat ttaccctgcg cgaacatctt
    10561 gccgggctgc ggaacaacga ccaactgctg ctgatgctgg tcatgtcttt cctgctgatt
    10621 aacgtcttta atattcgcgg cggtgggtat atgtacttca ttacctacgt cttacaaggc
    10681 agtacgggct acacgtcgct gttcttcacc atggtcacct tcgcctccat tatcggctcg
    10741 gtgattgtca gcccgttaac gcggcgtttc gataccgtca aaatttatta ctacaccaac
    10801 ctgctcctcg ctgcactggc ggtgttgatg tggttcctgc cctccggccc ggcttatcaa
    10861 acgctgtggc tggcggtgat cctcggtaat ggcgtgattc ttggcttcac attgccattg
    10921 cacttctcat tgatggcctt tgccgatgac tacggcgagt ggaaaacccg cgtacgttct
    10981 tccggcatga acttcgcctt caatctgttt ttcatcaagc tagcctgggc ctccagcgcc
    11041 gggatcatca gcctgctgtt tatttttgtc gcctaccagc ctggcgtgga aaaccagacc
    11101 gccagttcgc ttggcgggat cacggcgatg gaaacattgc tgcctgcgct attccacctg
    11161 ctgctggcag gggcgatccg cttttgcaaa ctcaataatc ctatgatgtc gcgcatagct
    11221 accgacctgc gtcagcgtca tgtacagcct taaggagcac aagatgaaca tttcggaagt
    11281 cgatctgcgt aaactgacgg tcagcgatcc gttcctcggt cagtaccaac aactggtccg
    11341 cgacgtggtg atttcttatc agtgggatgc cttgaacgat cgtatcccag aagcggaacc
    11401 cagccatgcg attgaaaact ttcgcattgc cgccggactt caggagggtg aattttacgg
    11461 gatggtgttt caggacagcg acgtcgccaa atggctggaa gcggtagcct ggtcgctgtg
    11521 ccagaagccg gacgccgaac tggaaaaaac cgccgacgag gtaatcgaac tgatcgcctc
    11581 cgcccaatgc gaagacggct atctcaatac ttactttacg gtaaaagcac ccgaagaacg
    11641 ctggagcaat cttgcggagt gtcatgaact ttactgcgcc ggtcatctga ttgaagccgg
    11701 agtcgccttc ttccaggcca cgggaaaacg acgcttgctg gaggtggttt gccgtctggc
    11761 cgatcatatc gaccgcgtat ttggtccaga tgaagacaag ctgcagggtt atcccggtca
    11821 tccggaaatt gaactggcgc tgatgcgtct ctacgaagtc accgaagagc cgcgctacct
    11881 ggcgctgacg aactattttg tcgaacagcg tggtgtgcaa ccgcactatt acgaccagga
    11941 atatgaaaag cgcgggcaga cctcgcactg gcacacctac ggcccggcgt ggatggtgaa
    12001 agacaaagcc tacagccagg cacatttgcc ccttgcgcaa cagcaaaccg ccatcggtca
    12061 cgcggtacgt tttgtctatc tgatgaccgg cgtcgcgcat ctcgcgcgtt taagtcacga
    12121 tgacagcaag cgtcaggact gcctgcggct gtggaacaat atggcccagc gtcagttata
    12181 tattaccggc ggcattggct cacagagcag cggtgaagcg ttcagcagcg attacgatct
    12241 gccgaatgac acggtatacg ctgaaagttg cgcctccatc ggcctgatga tgtttgcccg
    12301 acgaatgctg gaaatggaag gcgacagtca atatgccgat gtgatggagc gcgcactgta
    12361 caacaccgtg ctcggcggca tggcgctgga cggcaaacat ttcttctatg tgaatccgct
    12421 ggaagtacat ccaaaatcgc tgaaattcaa ccatatctac gatcacgtta aaccgatccg
    12481 ccagcgttgg tttggctgcg cttgttgtcc gccaaatatc gcccgcgtgc tgacctcgat
    12541 tggtcattat ctctacacgc cgcgtgaaga tgcgttgtat atcaacatat acgcaggaaa
    12601 cagcatggaa gtgccggtag aaaatggcac actgcgcctg cgggttagcg ggaactatcc
    12661 gtggcaggag caggtgacaa ttgcggtcga atcgccccag ccggtgcgtc atacgctggc
    12721 tttacgtctg ccggactggt gcacacagcc gcagatcata ttgaatgggg aagaggtcga
    12781 gcaggatatt cgtaaagggt atttgcacat tacccgcgaa tggcaggagg gcgatacgct
    12841 gaatctgact ttgccgatgc cggtacgccg cgtttacggt aacccgctgg tgcgtcacgt
    12901 cgccggaaaa gtggcgattc agcgcggccc gctggtgtat tgcctggaac aggccgacaa
    12961 cggcgagtca ctgcataatc tgtggctgcc caccgatgcg ccatttacga catttgaagg
    13021 caagggattg tttagccata agatcttaat ccaggcaccg ggttaccggt atgaacagag
    13081 caatccagag cagcaaccgc tgtggcatta cgacagcgcg ccagccaaac gccagccgca
    13141 aactctgacc tttatcccgt ggtttagctg ggctaaccgg ggcgaaggcg aaatgcggat
    13201 ctgggtgaat gaggaaaagc atcgccatcc ggaggttgga taaggcgctc gcgccgcatc
    13261 caacgtaggg agcacattgc ctgatgcgac gcttaacacg tcttatcagg catacgagtc
    13321 ggtgcgagag cgtaggccag ccgcaccgac atccaccggt gaattaaaaa cgccagctca
    13381 cgccggtgac aaaggtaaaa ctctcattac gatcaatcat cgggctgttg gtaatctctt
    13441 ccggcagctc gctgtatcct gcgctcgcca tcaatacgac gtgctctcct atcgggtatt
    13501 ttgccgtcag gctgacatag ggcacccagg catcctgcgc ggaataactt gccagaccgc
    13561 tacgacggga ctcactctct gaaatgccat agtaatagtc actgaaattc tcgtcataat
    13621 agagaacgcc cagcgcaggt gtcagcgaca ggcgacctat ctgcattttg tggaataccg
    13681 atagctcccc cacccagccg ttgctgttat ccagaacgtc cgcagccgca gaggctttca
    13741 cagttcccca acgctcgtgg tgataccagg caacccccgc catagccgta ctatcacgct
    13801 tatcaagctg ttgcatggca taatcgtcat tatcggcagg gtcaaattcc agcggcatcc
    13861 aggatgcggt caggctaagt tcattttttt cactttgcga cagaataaaa cctaacgtgg
    13921 tctgacgaac ataaaaagaa tcaccttcat aactaatcag gggaattgcc tttgtatttt
    13981 cattataacc gcgataaggc gattcattat aagccgcacc ggcaccaatg gataattcag
    14041 aagcagttgc gcttgccata aaaggcaacg caaataacgc cacaatattg cggttaatta
    14101 acatgttcgg tattccattt taaaataaga taaaaaagag tcggcatagt aatgcttaca
    14161 atttctccgg tgcaagattc atatttatat agtaatatat aaaattatat ataattgggc
    14221 tgttgcgatg acaaaattac aacttaagta ccgggagtta aaaattatct cggtaatcgc
    14281 tgccagtgaa aatatcagcc atgccgcgac tgtacttggc atcgcacagg ccaacgtcag
    14341 caaatatctt gctgattttg aatcaaaagt gggtttaaaa gtctttgacc gaaccacccg
    14401 gcagctgatg ctcacacctt ttggtaccgc gctgttaccg tacatcaacg acatgctgga
    14461 cagaaacgag cagcttaata attttattgc agattataag catgagaaac gtggtcgggt
    14521 caccatctac gccccaaccg gcataattac ctatttatcc aaacatgtaa tcgataaaat
    14581 taaagatatc ggtgatatca ccttatcgct aaaaacctgc aatcttgagc gtaatgcctt
    14641 ttatgaaggc gtcgaatttc ctgatgattg tgatgtttta atcagctacg ctccaccaaa
    14701 agatgaatcg ctggttgcca gttttattac ccaatatgcc gtaaccgctt atgccagcca
    14761 gcgctatctt gagaaacatc ccattagccg tcctgacgaa ctcgaacatc actcctgtat
    14821 tttgatcgac tcgatgatga ttgatgacgc gaatatctgg cgcttcaacg tcgccgggag
    14881 taaagaggtg cgagattatc gcgtgaaggg aaattacgtt tgcgacaaca cgcaatctgc
    14941 gctggagctg gcacgaaatc acctggggat tgtgtttgcg ccagacaaaa gtgtgcagag
    15001 cgacctgcaa gacggcacgc tggtgccctg ctttcagcaa ccctatgaat ggtggctgga
    15061 tctggtggct atcttccgta aacgggaata ccagccctgg cgggtccagt atgtgctgga
    15121 tgaaatgctg cgtgaaattc gccaccagct tgctcagtcg cagcaactgc ggcctgaaca
    15181 agcggcggaa agcgaagatt aatgatcgag ataaaggtaa tgcacccagc tggtcatgcg
    15241 taatagcact ttgcgcatca ccgagacatg gctgaaatga tcagagtaaa ccgccacctg
    15301 ggcgtaaata ccgtctggta aagcgctcaa atcagtgtgc tcatccagtt caatggtcgc
    15361 gataacgcca tctgaacccg gcgctgtgtt taacgtctgt aaggtgccgg tcgactgata
    15421 agctccgccg ggaacggctg gactaatggc tgccagctta ccgctgaata ccttacctgg
    15481 cagagcatta aacaccactt ccgcatcgtc gccaggagcc agtcgcagca aggagttctg
    15541 acggaactgc gccacgattt gtcgtttctg atcgggtata aacaccatca ccggacgtag
    15601 cggcagcgac gcggcatagg tacccggacg aatcagtacc tgggtcacat aaccatcgct
    15661 cggcgcacgc actatcgtct gctcaaggtt atattttgct tccgcgagct gtgctttcag
    15721 gctggcgatt tgagaatgtt cacccaacac caggctatcc agctggctct ggatctgttt
    15781 ttgttccgcc gccgatgact tcacggaggc ttcctgcgcc agataatttt gccgcgccac
    15841 atcgatatcg cgttctgaaa acgggtttac tttcgcctgg ctgccgcgtg cgtaacgctg
    15901 atactcttta gcgaatttat cccgcgtggc ctttgcctgc tgagtattcg ccgccatctc
    15961 atctaactct gcgcccaacg cccgctgttt atgttctgcg gtaacgatat ccgccatcag
    16021 ccgatccacc cgcgcctgat aacgcgtcgg gtccagtcga aaaagcacct cgcctttttt
    16081 aatcagcgta tttttcttgt ccgtcacttc gatcaccacg cctgtcacct gcgggacaac
    16141 tggaatagaa ataaccgctt tttgcgcttt aaaggtatac ggatggttat agttcattaa
    16201 cagaattaaa ccgctgacaa taaatatgcc acccagggcc gcggtgggaa tggtccattt
    16261 atttacagga attttgaaga tcttaaacat tgcccatgcg aaagccacat aggttaaaat
    16321 aatcagtaaa tccataagta ttactcggca gaagaggatt tgatgtcggc gagctgtttc
    16381 tccagcccgg caatacgctg ttgtagttgc acaacggagg agtcatggtt ttgcattccc
    16441 catccccgct ccgggcggta aagcgtggcc caaatccaca aaaatggcca gataacatgt
    16501 aacgtaaaaa gactcaccca accagcaaca tgaatggcgt cggcatgagg atgattacgt
    16561 tttttagcaa tcaggtaggg aatatcatgt aaaataataa tcccatagaa tatcaccaga
    16621 aatacaaaaa taagcactcc cagtgcaaaa tagtccagga acatatttcc ctcaaagaat
    16681 ataaaaaaga acaattaacg catattatga aatgccatga tgcagtgcaa tttccttatt
    16741 tatataacga catattgttt tttatatact ttcttaaatg tgctgtctgg tttttcaaca
    16801 ggacgaggtt tatcatattg aaatagtgga actattggcc aaactaatga ataactccag
    16861 taaaacatcc acagtacaga ttaagcgtat taaaccttca attatctacc gtttattgct
    16921 gattggcctc ggatcaccaa tggtgattta cggcctggtt cgcccgctca ccatcgaaac
    16981 gcgagattaa actcctgaca aaacacgccc cagaaacgca cttctgttgg cgtagtctgg
    17041 gttattgcgc acgtaggttt aaaacgtaaa ttgtaacaac gtgcgctttg tttatgccgg
    17101 atgcggcgtg tacgccttat ccggcctacg gtcaggttcc cgtaggcatg ataagacgcg
    17161 taagcgtcgc atcaggcaat gaatacccaa tgcgaccagc ttcttatatc agaacagccc
    17221 caacggttta tccgagtagc tcaccagcag gcacttggtt tgctggtaat gctccagcat
    17281 catcttgtgg gtttcgcgac cgatacctga ttgtttgtag ccaccaaacg ccgcatgtgc
    17341 cgggtaagcg tgataacagt tggtccacac gcgcccagcc tgtatgccgc gccccatctt
    17401 ataggccaga ttaccgttgc ggctccagac gcccgcgccc aggccatatt gcgtatcgtt
    17461 cgccagctcc agcgcttctt ccatcgtttt gaaggtggtc accgccagca ccgggccaaa
    17521 aatctcctcc tggaacaccc gcatattgtt ctgaccaaac agaatcgtcg gttcgaggta
    17581 gtagccgtct ttcagttcac cttccagcag cttgcgccgc ccgcctgtga gcacgtcagc
    17641 gccctctttt ttaccgatat caatgtagtt gaggatggtt tccagttgcc cgtgagaaac
    17701 ctgcgcgccc atttgcgtca cgctgtcgag tgggttaccg ctgcgaatgc tttcgacacg
    17761 gcggatggcg cgttccataa agcgttcgta gattgactcc tgcactaagg cacgactcgg
    17821 acaggtgcaa acttcaccct ggttaaaggc aaacagtgca aagccttcca gcgctttatc
    17881 gaaaaaggca tcttcttcat ccatcacatc agcaaagaag atatttggcg atttgccgcc
    17941 cagctccagc gtcaccggaa taatgttttg cgtggcgtat tgcataattt gttggcccac
    18001 ttccgttgag ccggtaaacg ccactttggc gatgcgtttc gaggtcgcca gatattcgcc
    18061 aatttcccca cctgcgccgt tgaccacgtt caccacgccc ggcggcagta aatcaccgac
    18121 gatttccatt agcagcagta cagaaagcgg ggtaagacgt gcgggtttca gcaccacaca
    18181 gttgcccgcc gccagcgcgg gagccatttt ccagctcgcc atcagcagcg ggaagttcca
    18241 cgggataatc tgccccacca cgcctaacgg ttcatggaaa tgataggcca cggtttcgct
    18301 atcaacttca ctgatcccac cttcctgcgc ccgaatacac gaggcgaaat agcgaaaatg
    18361 gtcaatcgcc agcggcacgt cggcagcact ggtttcgcga atgggtttgc cgttatccca
    18421 ggtttcagcc gtcgctaaca gctcaaggtt ttgttccatt cgatcggcaa tcttaaacag
    18481 aatcgccgcg cgatcctgca ccgaggtgtg cgcccattta tctttcactt tgtgcgcagc
    18541 atccagcgcc agatcgatgt ctcgtttgcc cgaagacgcc acttcgcaca gcagctgccc
    18601 ggtcaccggc gtcagattct ggtaatactc gccgtcggca ggggctaccc attcgccgcc
    18661 aataaagttg tcatagcggg cttttaactt gagggggaaa ccatactcgc cgggcttaat
    18721 ctgtgctgaa gggggattat tggtcatgat cgtctccttg cggtgtgagg tataacaagg
    18781 gtagacgtga ctggcgaaat cttcgccagt cggtaacagc tttacgacag ctatcacgaa
    18841 tttacgggca agggttacag agatagttga aaaagcggct gacaatttgc cagccgttgt
    18901 ggaaatgatg attacatcgc tgcgcgataa atcgccacaa tttcttcgtg agttgcctgg
    18961 atcgggttag taaaaccaca ggcatctttc agggcattag tcgccagaac cgcgaaatct
    19021 tcttctttca cgttcaggtc gcgtaggcct gccgggatat ccactttctt cgccagttca
    19081 cggatggcgt taatgcaggc ttcagcacct tccgcatcgt ttttacctgt cacgttcacg
    19141 cccattgcag cggcacagtc acgcagacgt gcggcggcga ctttgctgtt gaatacctga
    19201 acatgcggca gcaaaacggc gttacataca ccgtgtggca ggttgtagaa accgcccagc
    19261 tggtgcgcca tcgcatgaac ataacccaga gaagcattat tgaacgccat accggcgagg
    19321 aactgggcat aagccattgc ttcacgcgct ttcgcattac tgccatcttc aacggctaac
    19381 ggcagatttt cggcaatcat ggtcacggct ttcagtgcac aagcgtcagt gatcggcgtg
    19441 gcggcaatag aaacatacgc ttcgatagcg tgcgttaagg catccatacc cgttgcggcg
    19501 gtcagtgact tcggcatacc aatcatcaga gaggagtcat tgacagaaag cagcggagtg
    19561 acatgtttat caacaatcgc cattttgata tgacgcgctt cgtcagtgat gatgcagaaa
    19621 cgggtcattt cagaggccgt acccgccgtg gtattgatgg cgatcatcgg cagctgcggt
    19681 tttgcagagc ggtcaacgcc ttcgtaatcg cgaatatcgc cgccattggc tgccaccagc
    19741 gcaatacctt ttgcgcagtc gtgtggagaa ccaccgccta aggagatcac gctatcgcaa
    19801 ttattctctt taagtaattt caaacctgcg gcgacgtttt ccgtggtggg gttaggttgg
    19861 gtgccatcat aaataacgct aaaaatattg cgttcttcca gtgctttttg cacatcgccc
    19921 gccataccta atttcgttaa catactgtca gtgacaatta aggtacgggt aaatccataa
    19981 tctgccatca tattcattgc atcagtcaat gaatcagcgc cgatgacatt cacagaagga
    20041 ataaagaaag ttgaagatgc cataacacac tcctgaaagt gtaaaaaagg gtgggcagct
    20101 tactgaggat tttcattcga aaacatgatc attattatta attatcaaaa ttaatctaat
    20161 aaaaagcaca ttgtattcat taaacaatgt gcttttttta aaatcataag aaatttgtat
    20221 taatttattt taaaaattaa ttaatcattt ccttattttt ccggaaataa taatgcgtcg
    20281 cgcaataaat gatcatttcc acgacgacgc gtaaagccaa tgcggtcaaa atattccaga
    20341 atctgaattg ccagctttcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt
    20401 gaaccacact cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta
    20461 cggtaataac gatctttgac gatcgcggta attattcctt gctgcgccgc ctggcgtagc
    20521 gtcaggcgca ttgcctgctc gtcggtaccc gtctcttttg ccagatcacg cacccaccac
    20581 ggctcgtcac caaacagcgg ctctgctttt tgccagatgg cctgctgctc ttcgctgaag
    20641 cccgctttgt gatccggcag atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg
    20701 cgcatctttt caatcagcaa cagtactagc gcttcatctt ccattggcaa cgccatacgt
    20761 cgcagacgtt cgcgcccagg cccaggttca tcgcgatgtt gctcatgata agtcgctaat
    20821 gtgtcgagaa ttttccgttg ccagcgagcg gcaaccggcg cattcaacaa gctataacca
    20881 gcctgaatat agccaggctg ttgcagcaat tcgcgcatcc cttcgccgtt gagctggcgc
    20941 gcccaggcga aatccgcaag gttaaccgcg ccgcgttcca gatgaacaga taacgcatcg
    21001 gcatcgctct gcgcccgtgc aagagacgcc agccattgca gatattccgg cttacgttta
    21061 ccgcgacgcg gcgggttaag catcaccaca cgcgcccccg ccagcgtgtt gcgggccgag
    21121 atatcgcgca gcactaagcg atcgttatcc gccagccaca gcggcgtgtc gaacaccagc
    21181 tcggcaaggt tatcttccag cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga
    21241 atatgcagcg gctgccactg ggtcagcggt gtatgggttt gaagctcgac aatcacccgt
    21301 gtgaacggct ctggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt
    21361 tccgcatcac ccgcgatgtt aagcgcgata cgctgcccgg cattggcggt ttctgttggc
    21421 tggttttgcg catgcagcgc acgtacacgc atcggtttat ttacaccagt cagccagagt
    21481 gaatcgccta ccttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct
    21541 tttacggtaa atgcgcggtc aatagcgagg cggaaactat gttggctggc gtgctcgcgt
    21601 tccggcaact gaagcagatg ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg
    21661 gtgataaaca gttttgcctc agcaaaaccg tattcccgca gcacctcctt tacctggcgt
    21721 tcaacctcat taacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc
    21781 atcgggttac cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacgccg
    21841 tcatcgcacg ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg
    21901 gaaagaaact tttcatgacc gggtacgtcg ataaaaccag gcacgcgacc atccggctgc
    21961 ggccagtagg catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg
    22021 tcagcattta cgccagtaat cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg
    22081 gcagtcgcaa taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc
    22141 gtaaatccag ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac
    22201 gccagcgggc ggctaatgac tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta
    22261 atgccgcgct cggcaggcga tcaaccggca gcgaaccact gccaatctgc gaaagacatg
    22321 gcataacctg taccgcaaac tccgcgccgt aatgtgcggc aaggggggcc tgtaaacgtt
    22381 gtgcctggat ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt
    22441 cactcagagc ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca
    22501 ttttatccgc gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatctctt
    22561 ttttaccaac aataattcct gcctgcggcc cgcctaacaa cttgtcgccg gagaaactca
    22621 ccagactgac gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact
    22681 ggctaagatc gaccagcgag ccactgccta aatcagtcac tacgggaaca tccagctctt
    22741 tgccgagcgc caccagttcc gcttcatcta tcgctttggt gaacccctga atgctgtagt
    22801 tactggtatg tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat
    22861 tcgcgtgcgt gcggttggtg gtccctactt cgtgtagggt gcagcctgcc tgacgcataa
    22921 catcgggaat acgaaacgcg ccgccaatct ccaccagttc gccgcgagaa accaccacct
    22981 ctttcccgct ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattaacga
    23041 tacaggcatc ttccgccccc gtaatgcggc acagcaactg cgccagcgca cgatcgcgat
    23101 gtccgcgtcc agcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagcctgcg
    23161 caacggcttc caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg
    23221 ttcccgtcag gttgatcacc ggacgcagcg cgctctgcgc ttctttcgtc aaccgggcat
    23281 cgacttcttg cgcccagttt tcacaccacg caggcagcgt ctggctgcca cgaatcactt
    23341 ctcgcgcttc gtcgagcatc tgacgcaaca attccaccac gcgggtgtga ccataagtat
    23401 cacgcaaaga aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat
    23461 agagggaacg cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga
    23521 gaagggcgtt taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg
    23581 catcaagcct ttggcggttc ggtgcgagca aaactttcgc ggctaaacag gttttcgacc
    23641 agtttgacta agtgtgggcg atcgacacac cagcctggcg caacgcggcg gaaattgaga
    23701 tatccgacgg cacaggcaat ggcgatagtc gccagattga ccgtatcggt tttgagtgtg
    23761 ccatcgacca gatatccttc cagcacatcc agactgcggt tgattttctc ccgctggcgt
    23821 aacaattcat cttcagactg ctgcgccgct ggacgcgcct gttcacgcac cgataccagc
    23881 ccggcatcca taatgccatc cgccagtgcc tcaattttgc gcacccgcaa cgactccagc
    23941 ggatcgcgcg gcaacatcgc cggagcgaca ttcattaatt caatatattc agcgatgatc
    24001 ggcgaatcaa accaacattc gccctcttcg gtcaccagca ccggcacttt tcctaacggg
    24061 ttaaattgcg ccacgccgtt gtccgcgtta tagggcagtt cattaataaa ttcgaaagtt
    24121 atgccctttt ctaacaacag aatagaaagt ttgcgtacaa acgggctggt gtagctaccg
    24181 acgagtttca tgccgagtcc tttgtgcgag gaaaaatatc agtatggcct ggtgatggga
    24241 aaaagggcag aaaatgttga tggtttgtta cttccaaata aatcacatat ttatcatggt
    24301 gatataaata ttttcctaat tgtttctatc tgatggatat ctcacttaag gctttcttat
    24361 aaatctgtag ggttttgcct ggaagcagac aaataacccg ataaaacaag gatgagcaga
    24421 tgagcggaaa accggcagcg cgtcagggcg acatgacgca gtatggcggt agcattgttc
    24481 agggttcagc cggggtgcgc attggtgccc ccaccggcgt ggcctgttcg gtgtgccccg
    24541 gcgg
//
LOCUS       DAFATA010000063        24432 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFATA010000063 DAFATA010000000
VERSION     DAFATA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.064"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            452..727
                     /gene="ypdI"
                     /locus_tag="LGJ20_003031"
     CDS             452..727
                     /gene="ypdI"
                     /locus_tag="LGJ20_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBK2387061.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(804..1046)
                     /locus_tag="LGJ20_003032"
     CDS             complement(804..1046)
                     /locus_tag="LGJ20_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBK2387062.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1399..2319
                     /gene="lpxP"
                     /locus_tag="LGJ20_003033"
     CDS             1399..2319
                     /gene="lpxP"
                     /locus_tag="LGJ20_003033"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBK2387063.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2675..2746
                     /gene="ypdK"
                     /locus_tag="LGJ20_003034"
     CDS             2675..2746
                     /gene="ypdK"
                     /locus_tag="LGJ20_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBK2387064.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2811..4049)
                     /gene="alaC"
                     /locus_tag="LGJ20_003035"
     CDS             complement(2811..4049)
                     /gene="alaC"
                     /locus_tag="LGJ20_003035"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBK2387065.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4426..6123
                     /gene="ypdA"
                     /locus_tag="LGJ20_003036"
     CDS             4426..6123
                     /gene="ypdA"
                     /locus_tag="LGJ20_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBK2387066.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            6138..6872
                     /gene="ypdB"
                     /locus_tag="LGJ20_003037"
     CDS             6138..6872
                     /gene="ypdB"
                     /locus_tag="LGJ20_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBK2387067.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6885..7742
                     /locus_tag="LGJ20_003038"
     CDS             6885..7742
                     /locus_tag="LGJ20_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2387068.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7745..10240)
                     /gene="ptsP"
                     /locus_tag="LGJ20_003039"
     CDS             complement(7745..10240)
                     /gene="ptsP"
                     /locus_tag="LGJ20_003039"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK2387069.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(10265..11302)
                     /gene="ypdE"
                     /locus_tag="LGJ20_003040"
     CDS             complement(10265..11302)
                     /gene="ypdE"
                     /locus_tag="LGJ20_003040"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK2387070.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(11302..12387)
                     /gene="ypdF"
                     /locus_tag="LGJ20_003041"
     CDS             complement(11302..12387)
                     /gene="ypdF"
                     /locus_tag="LGJ20_003041"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK2387071.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(12402..13649)
                     /locus_tag="LGJ20_003042"
     CDS             complement(12402..13649)
                     /locus_tag="LGJ20_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBK2387072.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13671..13997)
                     /locus_tag="LGJ20_003043"
     CDS             complement(13671..13997)
                     /locus_tag="LGJ20_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBK2387073.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(14216..15181)
                     /gene="glk"
                     /locus_tag="LGJ20_003044"
     CDS             complement(14216..15181)
                     /gene="glk"
                     /locus_tag="LGJ20_003044"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBK2387074.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            15385..16641
                     /locus_tag="LGJ20_003045"
     CDS             15385..16641
                     /locus_tag="LGJ20_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBK2387075.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16756..17082
                     /locus_tag="LGJ20_003046"
     CDS             16756..17082
                     /locus_tag="LGJ20_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBK2387076.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(17223..18461)
                     /locus_tag="LGJ20_003047"
     CDS             complement(17223..18461)
                     /locus_tag="LGJ20_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK2387077.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18797..19999
                     /gene="nupC"
                     /locus_tag="LGJ20_003048"
     CDS             18797..19999
                     /gene="nupC"
                     /locus_tag="LGJ20_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBK2387078.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(20048..22237)
                     /locus_tag="LGJ20_003049"
     CDS             complement(20048..22237)
                     /locus_tag="LGJ20_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HBK2387079.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22446..22521)
                     /locus_tag="LGJ20_003050"
     tRNA            complement(22446..22521)
                     /locus_tag="LGJ20_003050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22486..22488),aa:Ala,seq:ggc)
     gene            complement(22561..22636)
                     /locus_tag="LGJ20_003051"
     tRNA            complement(22561..22636)
                     /locus_tag="LGJ20_003051"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22601..22603),aa:Ala,seq:ggc)
     gene            22857..23216
                     /locus_tag="LGJ20_003052"
     CDS             22857..23216
                     /locus_tag="LGJ20_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBK2387080.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            23218..23610
                     /locus_tag="LGJ20_003053"
     CDS             23218..23610
                     /locus_tag="LGJ20_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBK2387081.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23662..>24432)
                     /gene="gltX"
                     /locus_tag="LGJ20_003054"
     CDS             complement(23662..>24432)
                     /gene="gltX"
                     /locus_tag="LGJ20_003054"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK2387082.1"
                     /translation="ALKAPVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEA
                     LLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPE
                     YVATHLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADA
                     AKKHLRPVARQPLEVVRDKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVT
                     GAGQSPALDVTVHAIGKTRSIERINKALDFIAERENQQ"
BASE COUNT         6117 a         6437 c         5991 g         5887 t
ORIGIN      
        1 gggtttgctg aacttgctga gtttgctgtt gtgccgctac cggtgcatta tcagcagccc
       61 atacagttga agatgccaga attgctgtga ccatcgtagc cataattaaa cgtttcatac
      121 ttacctcatt ctttaacagt tttagaaaac gccgccatca tattgggtga gcatgaaatg
      181 agaaatcaga gagcaacggt cattttgaaa gggttatctt acagttgtag ttattatcgc
      241 aagaagtatt ttagtcttac aattgtagtt attatcgcaa gaagtatttt agtcttacaa
      301 ttgtaggaat ctgcttcctg tcgcagagaa ctattcttat gtaccacaaa gagatgtcat
      361 ttattatatc gatgtaaata ttgattaatt atatgtaaat acagccataa catcgtaggt
      421 tctcagtgaa ccattttcag gataactttc tatgaaagta aacttaatac ttttcagctt
      481 atttttattg gtctctatta tggcatgcaa tgtttttgca ttttccattt ctggtggtgt
      541 aagtgagagg agctataaag agactgaaaa aacatcagcg atgacaacca cacactctac
      601 aaaacttcag ccatcacagg cgattttgtt taagatgaga gaagatgcgc caccattaaa
      661 cctcacagaa gaaatcacgc ctacttatcc gacaaaggcg aattatctta ttcatcctgt
      721 gcgatagcgt ctgtcacatg gcaggtgaac aatcatgtct gatgctgatg tgaacacctg
      781 cccaaatgag ccatcctgac ccactatcgc ctgccatacc ccaccataaa gcgaatcatc
      841 aagccacaag ccggggcaag aaacataaat atccccgtca ctagttcatt accgccagtt
      901 aaccacatgg tgaagtaata aatgagtgca gcgaggatca ccatgccgaa gaaagaagag
      961 accgccgata ccacgttcag tacctgaccg atgtagccgc ttacgcagac aatacacagg
     1021 gcgagaaaca tccataaata aatcatagct caacctcctg ccgcgctaaa aaacctgtaa
     1081 cttcagtcac tttaatagta agacaatcct taacctctgg caatttttgc taagcatcaa
     1141 tgaaaacagc atgttaaatg caagactgtt gtgtacggaa aaatatttac tttgcacgat
     1201 taataatcag tagctgaaag cagtcagcga gtgataaagt acagataccg cattcatgct
     1261 cctcgtggtt atgtcctgac tagtctttac actctttaca ggaaccattg tcgtacatga
     1321 tggcccaacc aattgagtgt accgctgtcg acatggaatc ttctgcgata caacaattcg
     1381 tatctacaga aggtaactat gtttccacaa tgcaaatttt cccgcgagtt tctacatcct
     1441 cgctactggc tcacatggtt tgggcttggt gtactctggc tttgggtaca gcttccttat
     1501 cctgttctct gctttctcgg cacgcgcatt ggcgcaatgg cgcgaccatt cctgaaacgt
     1561 cgtgaatcta tcgcccgtaa aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa
     1621 cgcgagaaga tgattgccga aaactttcgt tcactcggca tggcgctggt agaaaccggc
     1681 atggcatggt tctggcccga cagtcgcgta cgtaaatggt ttgatgttga agggttggat
     1741 aaccttaaac gcgcacaaat gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg
     1801 tcgctggaac tgggcggccg cgtgatggga ctgtgccaac caatgatggc tacctatcgt
     1861 ccacataata atcagctgat ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa
     1921 gcgatgatcg gcagaaataa tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg
     1981 gtatggtttg ctcccgatca ggattatggt cgtaaaggca gctccttcgc gccgttcttt
     2041 gcggtggaaa atgtcgccac aaccaatggc acctatgttc tctcccgtct ctctggcgca
     2101 gccatgttga ccgtaacgat ggtaagaaaa gcggattaca gcggatatcg tttgttcatc
     2161 accccagaga tggaaggcta cccgacagat gaaaatcaag ccgctgccta tatgaacaag
     2221 attatcgaga aagagatcat gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt
     2281 aaaacgcgcc cggtgggaga atcgtcgttg tacatttaac aaactgctct tactctttcc
     2341 ctcctcaaat cggccataat aacctcatgg tttttatggc ttttttctta aggttttttc
     2401 cttcgctgaa tatctaatta ttaccgtcag aaattaaact gtcgccgata tgagacactt
     2461 gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc gtttcagttt taggcgtaca
     2521 ttagcgccgt ctcgagcagt ttagctcaga attctggggt gatgcccgcg gtaaaataca
     2581 gggcttttaa ggtcgctttg cccggtgtca actcactgta tcaggtgtaa tgaagtcatt
     2641 caggcgtaac agtaattacg cggagagatg taaagtgaaa tatttcttta tgggcatttc
     2701 ttttatggtc atcgtttggg ccggtacttt tgccctgatg atctaaaagc aaactgtgca
     2761 atgaaaaaca ggagccatca ggctcctgtt tgcatttctg acgacggtgt ttattccgcg
     2821 ttttcgtgaa tatgtttgct gctggcgggt agtaaaccgt cggcacggaa catcgcttta
     2881 atcccacgaa tcgcctgacg aatacggtcg cggttttcaa tcagtgcaaa gcgaacatgc
     2941 gtatcaccat agtcgccaaa gccaatcccc ggcgagacac aaaccttcgc ttcgttaagg
     3001 agcttcttgg caaattccag cgatcccatg gccgcatatg gttccgggat tttcgcccag
     3061 acatacatcg aagccttcgg catttcaacc atccagcccg cttcatgcag ccctttaacc
     3121 aatacatcac ggcggcgttt gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc
     3181 tccagcgccg caatcgccgc cacctgcaac ggcgtaaagg tgccgtaatc gtgatagctt
     3241 ttaatacgag ccagggcgct gaccagcgtt ttgttgccaa ccataaagcc gatacgccag
     3301 cccgccatgt tgtagctttt cgacagcgta aagaactcga ctgccacatc gcgtgcaccc
     3361 ggtacctgca tgattgacgg cgctttccag ccatcgtaga cgatatcggc ataggccagg
     3421 tcgtggacca ccagcacatc gtagcgtttc gccagcgcca caaccttttc gaagaactcc
     3481 agctccacgc attgcgcggt tggattcgac gggaagccga ggatcatcat cttcggtttc
     3541 ggataacttt cacgaatggc gcgctccagt tcgttgaaga aatcgacacc ttccaccagc
     3601 ggcactgagc gtacctgcgc cccggcaatc accgcgccat aaatatggat cgggtaactt
     3661 ggattcggca ccagcaccgt gtcaccatga tccagcgtcg ccagcatcag atgcgccagg
     3721 ccctctttcg aaccaatagt gacgatggct tctgattccg ggtcgatttc aacgtcgtag
     3781 cgatcctgat accagcggga aatggcgcga cgtaaccgcg gaatgccgcg tgaagtggag
     3841 taaccatgcg tgtccgggcg ctgggccaca gtacataatt tttcgacgat atgcggcgga
     3901 gtcgcaccgt ccgggttacc catgctgaaa tcgataatat cttcgccgcg ccgacgcgca
     3961 gccattttca gttcagcggt gatgttaaaa acgtagggcg ggagacgatc aatgcgcgta
     4021 aagcgacgtt cagggcgagt gtcagccata atttcctcag attaacgtta gcgcccggac
     4081 cgtccgagcg acgctgccac aatggtggct gttttgaaaa tagcctgatt aatttctgtc
     4141 tgtccagcgt tttagaacat ttttatcaat ggctttaaat gaggaactcc acttcaaaaa
     4201 caggaaattt ttctacgcta acaagaaaat taccaccaat tttattaaca ttataatttc
     4261 aataaattta ccagccagaa gtgcattatc aagagataca ttgctaatac accatttatc
     4321 tttcaatccc cttttaaatt tatggttttt cctcaaaaac ggcctgctgg tcatcagcct
     4381 tcaggttacc tatcatagag gtttaatcct tattcagagt cacccgtgca cgaaatattc
     4441 aacatgctgc tggcggtctt cgatcgggca gcgttaatgc ttatctgcct gttctttctc
     4501 atccgtatcc gcctgtttcg cgaactgttg cacaagtcgg cgcactcccc aaaagaattg
     4561 ctcgccgtta ccgccatttt ctcgctgttc gccctgttca gcacctggtc cggcgttccc
     4621 gtagaaggct cgctggtgaa cgtacgtatt atcgcggtga tgtccggcgg gattctgttt
     4681 ggcccgtggg taggcatcat taccggcgtg attgcgggta ttcaccggta tttaattgat
     4741 atcggcggcg tgacggcgat cccctgcttt atcaccagca ttctggcggg ttgtatatca
     4801 ggctggataa acctgaaaat ccccaaagca cagcgctggc gcgtcggtat tctcggcggc
     4861 atgttgtgtg agacgttgac catgattctg gtaattgtct gggcaccaac taccgcgttg
     4921 gggatcgata tcgtctctaa aatcggcatt ccaatgatcc tcggtagcgt ctgtatcggc
     4981 tttattgtgc ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag
     5041 gccaagctgg cgctggatat cgccaacaaa acgctacctc tgtttcgcca tgtcaatagc
     5101 gaatcattac gcaaagtttg tgaaattatt cgcgatgaca ttcacgccga tgcggtggcg
     5161 attaccaata ccgatcatgt gctggcctat gttggcgtgg gtgaacataa ctatcagaat
     5221 ggcgatgact tcattagccc gactacccgt caggcgatga attacggaaa aatcatcatt
     5281 aaaaacaatg atgaagccca ccgcacacca gagattcatt ccatgctggt gatcccattg
     5341 tgggagaaag gcgttgtgac cggaacgctg aaaatttact actgccacgc gcatcagatc
     5401 acctcgtcat tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac ccaactggag
     5461 gtttcacgcg ccgagcagct acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa
     5521 agcaaaatta atccccattt cctgtttaac gctttgaacg ctatttcatc atcaatccgt
     5581 ctgaatccgg atactgctcg ccagctgatc tttaatctgt cgcgttatct gcgctataac
     5641 attgaattaa aagacgatga gcaaatcgat atcaaaaaag agctgtatca aattaaagac
     5701 tatattgcca ttgagcaggc ccgctttggt gacaagctga cggttatcta tgatattgat
     5761 gaagaggtga attgctgcat tcccagcctg ctgatccagc cgttggtgga gaacgccatt
     5821 gtccacggta ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc
     5881 ggaaatcggg tacgcattgc ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt
     5941 gagcgggtcg aagcgaatga aatgccgggc aataaaattg gcctgctgaa tgtccatcat
     6001 cgcgtgaagt tattgtatgg cgaggggctg catatccgcc gcctggagcc ggggacggaa
     6061 attgcgtttt acattcctaa ccaacgcacc ccagtcgcct cacaggctac gttattgctt
     6121 tgagccggag tgatattgtg aaagtcatca ttgttgaaga cgaattcctg gcacaacagg
     6181 aactgagctg gctaattaaa gagcacagcc agatggagat tgtcggcacc tttgacgacg
     6241 gtctggacgt gttgaagttt ttgcagcata accgcgtcga cgccattttt ctggatatca
     6301 atattccgtc gctggatggc gtgttgctgg cgcaaaacat cagccagttc gcccataaac
     6361 cgtttattgt gttcatcacc gcgtggaaag aacatgcggt agaagcgttt gaactggagg
     6421 cgtttgacta cattctcaaa ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac
     6481 tggaagcggc ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg
     6541 agaatgacac cattaatctg gtgaaagatg agcgaataat cgtcacgcca attaacgata
     6601 tctattacgc cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt gaatcctacg
     6661 taatgccgat gaacattacc gaattttgca gcaaactgcc gccgtcgcat tttttccgct
     6721 gccatcgctc attttgtgtc aatctgaaca aaatacgcga aatcgaaccg tggtttaata
     6781 acacctacat tctgcgactg aaagatctgg attttgaagt gccggtcagt cgcagcaaag
     6841 tgaaagaatt tcgccagtta atgcatcttt aatcgggaac tttcatgaaa gcaccaggct
     6901 tgcccgccga tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac
     6961 tactggggcg cgtctggttt gccagccagc ctgcctcgtt accggtgggc agtttatgta
     7021 ttgattttcc ccgtctggat atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa
     7081 agcagcagcg aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac
     7141 cgatcaacaa caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt
     7201 tatcgtttta cgatagccgc accacgtcgt tgttgcatcc tgctcgccag atccagcttc
     7261 ccagcctgca acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat cttagccgtt
     7321 cgccgctgga gcagaatatc attcagccac tggtgttaag tttgctgcat ctttgccgca
     7381 acgtggtgaa tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc tatcacagca
     7441 tttgtaactg ggttcaggat aattatgccc agccgctcac ccgcgagagc gtggcgcagt
     7501 tttttaatat cacgcccaat catctgtcaa aactgtttgc tcagcatgga acgatgcgtt
     7561 ttatcgagta tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc
     7621 atctgtcaat tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac tatttttgtc
     7681 gcgttttccg gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt tttcagggct
     7741 aacgtcagaa ggttaattct gtttccagca gcgtcaggat actttgtgca tcttgcgcgg
     7801 caaacaacga ctggcggaag tttttattca ccagtttacg cgccagctgc gagaagactt
     7861 tcacatgatt catcccttcg ttagcaccca gcgtcagcat gatcaccagt tcgacttcgc
     7921 ccatttctga ctgccagtca atcggtttcg ccagccgggc aatgctgata ctggaatgac
     7981 ggatccactg agatttggtg tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa
     8041 tctcttcccg ctgccagaca tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa
     8101 cgccgaggtt gccgcacagg aactggatcg cctgctcttt attgctaaaa tcctgatcaa
     8161 caaagatatt ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta aaggcggtga
     8221 gtaacgcttc aatttcctgc gcactgcggc attcacatgc ctgacgcgcc agttcccgac
     8281 acgcctcgct atccagttga cgaagctggc ttttcaccgc cggaatacgc gggctactca
     8341 tactcagctc atccaggccc agcccaagca gtagcggcag ataacggctt tcaccgccca
     8401 gttcaccgca aatgcctacc catttgcccc gctgatgcgc agtggtaaca atctgctgca
     8461 acatgcgcag gaacgatggc gtaatcgggt tatacagcgg cgatacgcgc gggttattac
     8521 gatcgaccgc atacagatac tgggtcatat cgttagaacc gatactgaag aaatcgacct
     8581 catcgcagaa gtggtcgatg atgtaacaca ccgacggcac ttccaccatg atacccagag
     8641 taatcgtctc tgcatgacgc aggccatcgc gcttaagctc aacgatcgct ttttggatct
     8701 cgcccttcac ccataagatc tgatcgaggc tgtgaaccat cgggatcatc aactgggcgt
     8761 tgccaaaact ggcagcgcgc aaaatcgccc gtagttgagt gcggaacagg ccagcaaatt
     8821 ccgggtaaat acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg ggaatattca
     8881 gataaggaat gcttttatcg cctccgatgt ccattgtgcg gaagataatc ggcttgtcgc
     8941 ccgctgccag tagcacctgc tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt
     9001 cacgatccat atacagcatt tcggtacgga acagcccgac gccttccgcg ccgttggcaa
     9061 acgcgcctgg tgcttccaga gcggtgccaa tattcgccgc gatatcaata cgcttgttat
     9121 cacgggaata ggccagctgc gcggcagcct gcgcctgttg tttttggcgt ttatccgcca
     9181 gcgtctgcgc aacctgataa taaccgctca ccgcgtcatt cgggttaatc gccagcacgc
     9241 cgcactgggc gtcaagcacc gccggttgcc cggcataacg ggcaatcgca tccagcggca
     9301 ggccgctcag taccgggatc gccgaggcgc gggccagaat cagggtatgc gaggtgcgcc
     9361 cggttttctc cagaatcatg cccgcaagat ttttcaaatc aaggctcaaa aactggcttg
     9421 gggttaaatc ttcagccacc agaatggtcg gtttttccag caccagattg ttgcgcggct
     9481 tcagttccgg ccaggtgata tgcagcaact gttcgctaat atcgcgaatg tcgctaacac
     9541 gttcgcgcag ataatcgctg gcagaagcag aaagcttggc gcaaacctgc tccatattgc
     9601 tgatgatcgc cgcccccagt ccctgatgct gttctgtcat caggcgacgg atattgcctg
     9661 caaattcatc atcctgaatc agcgacaaat gggcgctgag gatagttttg ctttcgccgt
     9721 cacgctcacg caattgctgg ttcagttgct cggcaagcgt tgccaggctg tgctccagcc
     9781 gggtggaatc ttgcacactg gcggggattg cccgataact gtcgaggctg tcgctttgta
     9841 acagggtcag cgtacccacg ccgacgccgc ttgccagcac attgccgtac agtaaatccg
     9901 ggttaaggcg gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc tgcgtaggct
     9961 ggacgctgtc gctgtcgata aagcgcacct ggatgtactc ttccagcacg cgccgcgcct
    10021 gctcttcatc gctgccgcta atgttcaggc tgcaactgtc attaaatagg gtgccggtgc
    10081 caatcagcgc cagcgaactt ttcgcatcgg cctttgcgtt ctggcgatgg ttaataaagg
    10141 tgacttcact ttgccactgg ctgcactgtt ctttaagttc ccacgccgga cgagcatgca
    10201 gaccgttagg caaaggacag agaaattgaa tcgttaacat aaccgctccg ggttaatcag
    10261 gagatcatct gaaatccgtc agttgaacaa ccgtctcacg ggtaagacgt tgaataaggg
    10321 cagataaaag ttgctgcatc tgcaaaatgt cgcggcaatc ggcaatcgat gccgcacaat
    10381 gtccgtggcg cgtcgctggc cccatcacca ccgtgggcac gccggtgccg gttaaatgca
    10441 ccgctccgcc gtccgtgccg ccgttgctga acatatccgc ctgcaacggc acgccaattt
    10501 ctgccgccac ggtttcgatc caggcggtaa gttttggcgg cgcaatcagc gacttgtcgc
    10561 ttaacaccag catcggcccg ttaccaatct ggcgatggtt agccgcgcta taatcaaagt
    10621 ttttcgccca gcaggcggta tcaagcacaa tggcgacatc cggcgacacc gcgcgggtgg
    10681 cagtttgccc gccgcgtaat cccacctctt cgctggaact tgccaccagc cacacctccg
    10741 caggcagttc ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag caaccgaggc
    10801 ggtcatcaaa ggctttcccc atcacccgct ggtgagggag aacctgaaaa gttgtatcaa
    10861 aggtgacgcg atcgcctgga cgaattcccg cctgcatcac ttcgtcatag gagcgcgcac
    10921 caatgtccac gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg
    10981 gaattttgca ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg gcagccatgc
    11041 gcacgttgcc aaccggcagc acgtcaatag cgccttcgcg ggagatgctg cgcaccataa
    11101 atcccacttc gtccatatgg gcgcagatca tcaccttcgg acctgtcgat tcattgaggc
    11161 ggatcagcac cgatcccaga ccatcaaatc gcacttcttt ttgcaggcga tccgcttctt
    11221 ccagtaggat ctgccgcact tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg
    11281 cttttaatag cgataaatcc attatgcctc tcccgtgagc aacactgttt tcggcatggc
    11341 gtagagcact tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc gcacgccccc
    11401 ttgccctggc aaataaatcc ccggctccac ggtcagcaac atgcctggct gtagcgtcgt
    11461 ggtgtcccgc ggtgaaaaac gcggatcttc atggacttca atgccgatag cgtgaccagt
    11521 gttatgaccg aaatagtcgc caaatccagc ctcggtaatg acccggcgcg cggcgtcgtc
    11581 aatctgctgg cagcgcacgc cggggcgaat tgcagagatt gccgcgagct gtgcctgcag
    11641 gacaatctga tagacgtcaa acagcgggtg agattcggcg ctcacccctt cgccattcac
    11701 cagcaaggtg cgcgtcatat cagagcagta gccctgatac agcgcaccaa aatcgagagt
    11761 gacaaactcg cccgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccgcg
    11821 ccagccgctg gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa
    11881 ccactccagt tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat
    11941 atgctccgct ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg
    12001 cgttttgatt tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga
    12061 cttccagcgg tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa
    12121 ctgctcatcg gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa
    12181 atgcagctgg tagccttgcg cacgggcttc cacctcaacg taatagcgcg aatccaccag
    12241 aatgtgcgca ctttcacggc taatcaccac ataacctgat ccggtggaga tccccagatg
    12301 cggctgtttg ttctgccgtg aggagagaag cactgcatcc agttgttgcg ccttaagcca
    12361 gtcgcgcagc gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac
    12421 aatttgcctt tacggaacat catcagacgc aggaacacca ccatcagcgc agttatgact
    12481 gcccccagcg cgatccccgc catatagacg cccaggttag tgaccagcgg ccatgcccag
    12541 atagcggact ccgggaacca ttgcactgcg cccagccaga cagcggcggt tgagccgaca
    12601 atagcgccga ccatatacga cggaatggcg gtgatggggc tttccagcgc aaacggaatc
    12661 gcaccctcac tgatccccat aaaggcaagg aacatcgcgg tcttaccctg cggataaagt
    12721 tgagcgttga acaggcgttt gccggttaaa cgacggtcaa taatggtcgc cagacccaga
    12781 ccaatcggag gaataacgat agcgatagaa cgagcggtga ccggtaatac gtggtcagtg
    12841 gtaaagctga aggcaacgaa acctgcggct ttgttgatcg ggccaccaag gtcgattgct
    12901 gtcgccgcgg cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc
    12961 acagtacgga taccgccgtt gatccagccg ccaaacgggg tgatgacgta gtacatcgcc
    13021 agcatgacaa aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg
    13081 aaatccggta actggatttt ctggttcatc catttcacaa ggtaaccggc aacgatagaa
    13141 ataatcagcg cgccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg
    13201 ctgggatcga agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca
    13261 gccgggaacg ccagtttgcc accgatagag ttcgccacaa aagcagcaaa catcggaatg
    13321 gcaaaaccaa acaacacgcc gccgaaggac tgcgacagcc aggcaaattt cagtaaggag
    13381 aggtcgaaac cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga
    13441 attttaagcc agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt
    13501 aaggtcggca ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag
    13561 ctgcttttag aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca
    13621 acggttgcac tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt
    13681 tcagaggcaa tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc
    13741 tgcaaagtga tttcataaac gtcgcgtgat tcgaaacgct cattatcttc cggggtaact
    13801 gccacggagt ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg
    13861 ataccgtccg cgccctgggt ttcaattttc acttcataac cggcttctac cgccgcttct
    13921 tccagcgcct gagcggccat aaaagtgtga gccaggccca ttgggcaggc acataaggca
    13981 attagtttct tactcatcgt taattcctca ttcagtgata gttgacgagt gaaattatga
    14041 acgtgcaaaa caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg
    14101 ctgtacggaa gtgtgacgca ggtcggtctt gtgaggggac acaaagaaaa attgaattaa
    14161 ctgtatgatt taaaagatta tcgggagagt tacctcccga tataacagga aggatttaca
    14221 gaatgtgacc taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat
    14281 ggacgatgag atacaccgga atatcataga catattcttt aaagcgccct ttatcttcaa
    14341 atgcggcacg gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgcccg
    14401 caataaacac gccgccaaat gtcccgagat tgagcgccag attgccgcca aaacggccca
    14461 taatgacgca aaacagcgac aacgcgcggc ggcaatcggt gcagctgtca gccagcgcac
    14521 gttcggtaat atcttttggc ttgagatttt ctggcaggcg gttgtcagct ttcacaattg
    14581 cgcgatacaa attcaccagc ccagggccag aaagcacgcg ttccgccgaa acatggccaa
    14641 tttccgcacg cagtatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa
    14701 cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa
    14761 ccccaagccc tgttccggca ccgtaaaccg caataggctt accttctacc ggttctgcgc
    14821 caccaaactg aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa
    14881 aatcgttaat aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga
    14941 acgcccaggt atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa
    15001 tacagccgtc tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga
    15061 ggctggggta atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat
    15121 cacacagagc aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca
    15181 ttcttcaact gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg
    15241 cataacaaca acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta
    15301 ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat
    15361 ctgaccccgc ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta
    15421 tcgctccccg ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa
    15481 atcgcctcgg tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc
    15541 caggattcac ctctctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg
    15601 gttatccgtt tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc
    15661 gcaccagttc cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct
    15721 ggcggcgtca gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgcggtt
    15781 gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct
    15841 gccggaacca tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa
    15901 accttaaatg gcagtagtga agttccgcta tgggatcgtc tttttgcgcc attaatggcg
    15961 gcagcagctg gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt
    16021 gcgcattacg gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt
    16081 gccatcgcag caggaatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgcat
    16141 caaatgaaaa atccggtgct cgtgctaggt attggcggat ttattctcgg tattctggga
    16201 gttattggtg ggccggtttc gctgtttaaa gggctggatg agatgcagca gatggtggca
    16261 aatcaggctt tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg
    16321 gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc
    16381 gtggcattag ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt
    16441 tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt
    16501 atggcggcag tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt
    16561 ccggcatggc tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag
    16621 ccaccccacg ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga
    16681 tgatacgggg ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg
    16741 tgaaggagaa taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc
    16801 ccgcttgcag caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc
    16861 gatcgcgatc attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg
    16921 catcgtaatt atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt
    16981 ggctgggata agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac
    17041 gatcatcgcg gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag
    17101 cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc
    17161 gtagggcgga taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc
    17221 aactacaatc ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact
    17281 acaatcaccc agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg
    17341 ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc
    17401 acctgactca taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac
    17461 ggcaacatgg tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc
    17521 atcaccacct gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga
    17581 cttaacccaa agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc
    17641 tcatcaagat cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc
    17701 atcatcgcca gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg
    17761 gcggaataac gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa
    17821 taaatcacat gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcttcc
    17881 gaagtaggta aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggaa
    17941 aaaatcaact cgacaatgta agccgcggca acaaacaaca gtaacccacc aatcactttc
    18001 tccagcggtt tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc
    18061 agcaccgcgc cctgcaacaa cgaaacacca agaatgagtt taaaaccgat cgccgcacca
    18121 ataaattccg ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac
    18181 actacgggac gcggatagtg atcacgaatc tgctccgcca gatttttacc ggtggcaatc
    18241 cctagtttgg cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc
    18301 cacagtagct gatagccgaa gctagcaccc gcctgaatat tggtcgcaaa gttaccggga
    18361 tcgatataac caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc
    18421 gccgcccgtc cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata
    18481 gcctttgcta tgtttcatgc tatgccaaac gagaatgatt atcaaattca tttgaatggg
    18541 ttgtggtgat ttctttgata gaccaggatt atgactacga aaagattgat gacttgaatg
    18601 ttatggttat gtttaatgtt agcacattta cataactttc agcttccata cacaacatag
    18661 cagaaatgta tgacagatca ctatttttga agcttgtcac aggacgtcat tatagtgtgt
    18721 gtcagatctc gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct
    18781 catatttgga gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc
    18841 gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca
    18901 actgcttgtt atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg
    18961 cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc ggatttgcca acgaagggac
    19021 taacttcgtc tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct
    19081 gtgcccaatc gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc
    19141 ggtgatcatc cgcgcaattg gtttcctgct ctccaaagtc aacggcatgg gcaaactgga
    19201 atcctttaac gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa
    19261 agatatcctc ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc
    19321 caccgtgtcg atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt
    19381 tgctgcgctg gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta
    19441 ccgtgttgat gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt
    19501 cttcgaaatg ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc
    19561 gatgctgatc ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac
    19621 tggctggttt ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat
    19681 tgcatgggtg atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac
    19741 caaactggtt tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct
    19801 ctctccgcgt gctgaaggca taatctctgt gttcctggtt tccttcgcta acttctcttc
    19861 aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc
    19921 tcgcttcggt ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat
    19981 cgcagcactg gtgctgtaaa accatacata aaaaagccgg ggatcattcc cggctttttt
    20041 acgcctgtta atcagctaat ggctgcgggc gaccaatcaa atacccttgc agatattgca
    20101 ccccgagctt atgcaatagc gcctgctgct gttgcgtctc gacaaactcc gcgaccacac
    20161 tcaatgactt cgctttcgcc agatcggtaa ttgatctcac aatcatcgca tccagcgtgt
    20221 tcgtgacaat atctttcaca aagacgccat caattttgat gatatcagcc tgcaaacgct
    20281 ttaaccgttc gtagttggca tatccggtgc caaagtcatc aatcgcaatc cggaaaccaa
    20341 acttatgcag ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac gcctgctcct
    20401 cggtgatctc aagaatgacc gcctgcgggg agatgtgata acgcttaaac agacgaataa
    20461 tccgcccggc aatattcttt tgcagcagcg tgagcggcat taaattgact gaaaagcgcg
    20521 gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc agcacttgca
    20581 aatcaaaacg cgcgctaagg ttaaactgag caataagggg cagaaactta tccggggtca
    20641 taatgccgcc gtcatatttc agtcgcgcga ggatctcatc ataaccttca ccctctttgt
    20701 tgcgaattgg ctgggcgtag agcagcaaat caccctgatc taacgccgtg cgaatggtat
    20761 tcagcaatag cacctgttta gtggtctgcc cggaaaccat ctcctctctg ctatccagcg
    20821 ccagcacatg atgatgtgcg caggattgct ccgccagcca gcttaactgc cccaacaagg
    20881 gttgcagggt ttcctgattt ccatcaaaac gcccccaggc agcaccatag cccatatcca
    20941 gcccggtatt gttccagtga atttgccgac tattcaggat gttaaccata tgctggagtc
    21001 gcccttccgt ttccggcccg ctcagcacta acagcagttc actacccggc aattgataca
    21061 acttttcgtt ttcctgcatc aacggcagca gcgtacggca aattgagcgg atacagtgaa
    21121 cgcgcatcat taagccgtaa tgacgactca taaactcaag attatcaatg cgcaggcagc
    21181 aaaaactctt gcccgcctct tgctccggcg cttgttccaa cgcacgaaag ttgggtagga
    21241 gcgttaacgg atctgtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat
    21301 gataaatgcg caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa
    21361 atgccagcga atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagcaggc
    21421 aaagcgttga aacagcccag gtgagattta aaaacggata gcgaagctta ccgaccccga
    21481 gggtaaagat gatgaagaaa acgggtacca agtaaccggc aataaagtcg ttttcataag
    21541 gtgtgcacaa cagaagtagc agcacgctta acgctgccag ccagcttaag gtaaatgcgc
    21601 gtttctcttt gcccaacgac ggagcgatat ccctgcgcca caatatctgc gcaaagtggg
    21661 gacttacaat catgcgagtg agatagtaga aaagcatgtt gtaaatcagc acagcggtga
    21721 aaaggcttag caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata
    21781 tctttagcgg gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg
    21841 gcgtcaccaa accaagccag aacagacgtt gccagacata tcggctggtc aatccataac
    21901 gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct
    21961 ggctaccgag catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc
    22021 aaaacatccc gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca
    22081 gcattacgca cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg
    22141 gcgaaataaa acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata
    22201 tcttgatatt ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg
    22261 gggttttcag gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc
    22321 ctgttgccga tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac
    22381 tgtattacaa aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg
    22441 aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc
    22501 tcccagctga gctatagccc cacgatgcgt ttacgtacca agtttgctgg gtgcaaaatt
    22561 tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca
    22621 gctgagctat agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt
    22681 ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc
    22741 aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt
    22801 aaagtggtgt tataaaatga actactaata gacccacata cattcaggga attgttatgt
    22861 tcaaggagag gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca
    22921 ttaataaatg ggtacgcaag gaaggctgga caacgtcacc aaaacctggc gtccagggtg
    22981 gcaaagcccg actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac
    23041 gtccagaagg tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc
    23101 tggtgacact ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc
    23161 tgcgggaagg gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg
    23221 aaaagattac gcaacaaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa
    23281 caaactgtta accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg
    23341 aaaggcggtc gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac
    23401 acgccagcct tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac
    23461 gctcctggag cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca
    23521 gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc
    23581 gcccgtctcg atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc
    23641 gtttatcttt ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc
    23701 tttgttgata cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc
    23761 tggagactgc cccgcaccgg ttacggcgac acgcagcggc ataccaactt tacccatacc
    23821 cacttccagc tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc
    23881 agtaatcgcg gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag
    23941 atgttttttc gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct
    24001 ctgtgccatc tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag
    24061 ctgcgggccg ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc
    24121 aacatactcc ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt
    24181 gttgaacgca ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc
    24241 acgagtgaag atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag
    24301 tgcttctggc aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt
    24361 ggacagtttt ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg
    24421 cgctttcagg gc
//
LOCUS       DAFATA010000064        23887 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFATA010000064 DAFATA010000000
VERSION     DAFATA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.065"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1135)
                     /gene="ydcO"
                     /locus_tag="LGJ20_003055"
     CDS             complement(<1..1135)
                     /gene="ydcO"
                     /locus_tag="LGJ20_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBK2387083.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAGAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGI"
     gene            1188..1724
                     /gene="sutR"
                     /locus_tag="LGJ20_003056"
     CDS             1188..1724
                     /gene="sutR"
                     /locus_tag="LGJ20_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBK2387084.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPATPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1797..3758
                     /gene="rlhA"
                     /locus_tag="LGJ20_003057"
     CDS             1797..3758
                     /gene="rlhA"
                     /locus_tag="LGJ20_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBK2387085.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3850..4080)
                     /locus_tag="LGJ20_003058"
     CDS             complement(3850..4080)
                     /locus_tag="LGJ20_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBK2387086.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4302..4478
                     /gene="hicA"
                     /locus_tag="LGJ20_003059"
     CDS             4302..4478
                     /gene="hicA"
                     /locus_tag="LGJ20_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HBK2387087.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4524..4940
                     /gene="hicB"
                     /locus_tag="LGJ20_003060"
     CDS             4524..4940
                     /gene="hicB"
                     /locus_tag="LGJ20_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBK2387088.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5019..6425
                     /locus_tag="LGJ20_003061"
     CDS             5019..6425
                     /locus_tag="LGJ20_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK2387089.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6670..7815
                     /locus_tag="LGJ20_003062"
     CDS             6670..7815
                     /locus_tag="LGJ20_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2387090.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7833..8846
                     /locus_tag="LGJ20_003063"
     CDS             7833..8846
                     /locus_tag="LGJ20_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2387091.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8847..9788
                     /locus_tag="LGJ20_003064"
     CDS             8847..9788
                     /locus_tag="LGJ20_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2387092.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTTFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9778..10572
                     /locus_tag="LGJ20_003065"
     CDS             9778..10572
                     /locus_tag="LGJ20_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2387093.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAARRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10594..12018
                     /gene="patD"
                     /locus_tag="LGJ20_003066"
     CDS             10594..12018
                     /gene="patD"
                     /locus_tag="LGJ20_003066"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBK2387094.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12115..12210)
                     /gene="yncL"
                     /locus_tag="LGJ20_003067"
     CDS             complement(12115..12210)
                     /gene="yncL"
                     /locus_tag="LGJ20_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBK2387095.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12405..12578
                     /gene="ortT"
                     /locus_tag="LGJ20_003068"
     CDS             12405..12578
                     /gene="ortT"
                     /locus_tag="LGJ20_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBK2387096.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12664..12897
                     /locus_tag="LGJ20_003069"
     CDS             12664..12897
                     /locus_tag="LGJ20_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBK2387097.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(12898..13347)
                     /locus_tag="LGJ20_003070"
     CDS             complement(12898..13347)
                     /locus_tag="LGJ20_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2387098.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13344..13862)
                     /gene="mddA"
                     /locus_tag="LGJ20_003071"
     CDS             complement(13344..13862)
                     /gene="mddA"
                     /locus_tag="LGJ20_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBK2387099.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14043..15080
                     /gene="curA"
                     /locus_tag="LGJ20_003072"
     CDS             14043..15080
                     /gene="curA"
                     /locus_tag="LGJ20_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBK2387100.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15278..15943
                     /gene="mcbR"
                     /locus_tag="LGJ20_003073"
     CDS             15278..15943
                     /gene="mcbR"
                     /locus_tag="LGJ20_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBK2387101.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(15979..18081)
                     /locus_tag="LGJ20_003074"
     CDS             complement(15979..18081)
                     /locus_tag="LGJ20_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBK2387102.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            18323..19384
                     /locus_tag="LGJ20_003075"
     CDS             18323..19384
                     /locus_tag="LGJ20_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBK2387103.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDASNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19499..20998)
                     /gene="ansP"
                     /locus_tag="LGJ20_003076"
     CDS             complement(19499..20998)
                     /gene="ansP"
                     /locus_tag="LGJ20_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBK2387104.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21264..21881
                     /locus_tag="LGJ20_003077"
     CDS             21264..21881
                     /locus_tag="LGJ20_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBK2387105.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            21957..22169
                     /locus_tag="LGJ20_003078"
     CDS             21957..22169
                     /locus_tag="LGJ20_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HBK2387106.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            22990..23887
                     /gene="tssI"
                     /locus_tag="LGJ20_003079"
                     /pseudo
     CDS             22990..23887
                     /gene="tssI"
                     /locus_tag="LGJ20_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         6072 a         5825 c         6117 g         5873 t
ORIGIN      
        1 caatcccgac cagcgtcaat ccacttgccg ttaccagaaa tgccaccacc gccgcgtctc
       61 gctcacgctc attatgcagc gcctgataca aactgccgcc gatggtactt aacagcgcca
      121 gacctgccag catctggatc caacttacgg gcagggcagc catcatcccg gtaatggcac
      181 taccaaacag acctgcgagc aaatagaaaa tgcctgcacc ggcagctgcc agccaacgtt
      241 gatctttatc cggatgcgct tccgggcttt ggcaaatagc cgcggtgatt gccgcaatac
      301 cgacggaata aacgccgaaa ggggaaaaaa ccagtgccag caatccagta aatacaatta
      361 atggcgaaac aggagttgaa tatccggctg ctttcattgc tgcgataccc ggtgcgtttt
      421 gcgatgccat cgtcaccaga aaaaggggga gtgcaacgct caggctgtga gcaaacgaaa
      481 aatcaggggt aatataagtg gggagaacgg gtttaaagac aacatcagtt gtgacaacgt
      541 caccttgcgc gataacgatc acgatcccaa taatcatcgc ggcaattacc gcatagcgtg
      601 gcgcaacggc cttggttgcc agccatacca gcaacatact tccacacaac gtaaattgac
      661 cgtccagact ggcaaacgcc tgtaaaccaa agcgtaataa aatcccggca agcattgccg
      721 ccgcaagcga gtgcggaata atgcgcatca gacgagcaaa gagtcccgtt atgccgcaga
      781 ggactattaa cgcgttggtg acaataaaaa cgccgatggc ttcgttaagt gttagtccct
      841 gcaatccggt gaccaacaaa gccgcgccag gcgttgacca tgcggtgaga acaggtacgc
      901 gataccataa tgtcagagtc agcgtactga cgcccattgc cagccccagc gccgtcatcc
      961 agccagagat ttgtgcagtg gtggctccgg cgacaatcgc tgcttgccag attattgccg
     1021 ctgaactggc gtagccaatt aataccgcca gaaaccccgc cagtagcgtg ggtggaggaa
     1081 cagaaaacag acgcatagtc acctcgtgcg ttatagcgta caggcaccgt agcatttgtc
     1141 cgttataacg cacaagtgat aaacttccgt tttgccggag gagtcgcatg gaaaatctcg
     1201 ctcgcttttt atccaccaca cttaaacaac tacgccagca gcgcggctgg agtctttcgc
     1261 gactggcaga agcgacgggc gtttctaaag caatgttagg gcagattgag cgtaatgagt
     1321 ccagtccgac ggtcgcgaca ttatggaaaa ttgccaccgg gttgaacgta ccattttcaa
     1381 catttatttc tccgccgcag cccgctacgc cttcagttta tgatccacaa cagcacgcga
     1441 tggtgatcac ttcgctgttt ccttatgatc cacaactctg ctttgaacac ttctcgattc
     1501 agatggcatc tggtgcaata agcgaatcga cgccccatga gaagggggta atcgaacatg
     1561 tcgtcgtcat tgatggacaa cttgatctgt gcgttgatgg ggaatggcag acccttaatt
     1621 gtggggaagg cgttcgattt gctgcagacg tcacgcatat ctatcgtaat ggtggggagc
     1681 aaaccgtaca ttttcattcc ctcatccatt acccgcgcag ttaagcggga aaactatttc
     1741 gcaacgtcgc gcttgtggct aaaatagccg ccatttttca gctactggat aagaatgtga
     1801 ccgtatcttc tcatcgactt gaactgttaa gcccggcacg cgatgccgcc attgcccgcg
     1861 aagctatttt gcacggtgcc gatgctgttt atatcggcgg ccctggtttt ggtgcccgtc
     1921 ataatgccag taatagcctg aaagatattg ccgagctggt gccgtttgcc catcgttatg
     1981 gtgcaaaaat tttcgtcaca cttaacacca ttttgcatga tgatgagctg gaacccgcgc
     2041 aacggctgat taccgacctc taccagaccg gtgtcgatgc gctgattgtt caggatatgg
     2101 ggattctgga acttgatatt ccgccgattg aactgcacgc cagtacgcag tgcgacattc
     2161 gtacagtaga aaaagcgaag ttcctctctg atgttggctt cacgcagatt gtgctggcgc
     2221 gagagctgaa tcttgagcag atccgcgcga ttcaccaggc cacggacgcg accattgaat
     2281 tctttattca tggggcactg tgcgtggcct attcgggtca gtgctacatt tctcatgcgc
     2341 aaacagggcg tagcgccaac cgtggtgatt gctcgcaggc gtgccgtttg ccatacacat
     2401 tgaaagacga tcaggggcgg gtggtttcct atgaaaaaca tctgctgtcg atgaaagata
     2461 acgatcagac tgccaacctc ggcgcgctga ttgatgctgg cgtacgctcc ttcaagattg
     2521 aagggcgtta caaagatatg agctacgtga agaatatcac cgcccattat cgccagatgc
     2581 ttgatgccat tattgaagaa cgtggcgatc tggcgcgcgc ttcatcaggt cgtactgaac
     2641 atttctttgt tccatcgacg gaaaagactt tccaccgtgg tagcacagat tattttgtga
     2701 atgcccgtaa aggcgatatt ggcgcgttcg attcgccgaa atttatcggc ctgccggtag
     2761 gcgaagtatt gaaagtggcg aaagatcatc tcgatgttgc cgtcaccgag ccactggcaa
     2821 atggtgatgg cctgaacgtg atgattaaac gtgaagtcgt cggttttcgt gccaatacgg
     2881 tcgagaaaac cggagaaaat cagtaccgcg tctggcccaa tgaaatgcca gcagatttgc
     2941 acaaaattcg tccacatcac ccactaaacc gtaatcttga tcataactgg cagcaggcac
     3001 tgacaaaaac ctccagcgaa cgtcgggtgg cggtagacat tgaactgggc ggctggcagg
     3061 aacaactgat tctgaccctc accagtgaag agggtgtcag catcacgcat acgctggacg
     3121 ggcagttcga cgaagccaat aacgccgaaa aagcaatgaa caatctgaag gatggtctgg
     3181 caaaactggg gcaaaccctc tattacgccc gcgatgtgca aattaatttg ccgggggcgc
     3241 tgtttgtacc aaacagtctg ttaaaccagt tccgccgtga agctgctgac atgctggatg
     3301 ctgcgcgtct tgccagttac cagcgcggca gccgtaaacc ggttgctgat cctgcgccgg
     3361 tttatccgca aacgcatctg agtttcctcg cgaacgtata caaccagaaa gcgcgtgaat
     3421 tttatcatcg ctatggtgtg cagctgattg acgcggcgta tgaagcacat gaagagaagg
     3481 gcgaagtccc ggtgatgatc accaagcatt gtctgcgctt tgcctttaat ctgtgcccga
     3541 aacaggcgaa aggcaatatc aaaagctgga aggcgacgcc aatgcaactg gttaacggcg
     3601 atgaagtatt aacgctaaag tttgattgcc gcccatgcga gatgcacgtc attggcaaaa
     3661 tcaaaaatca catactgaaa atgccgttac cgggaagcgt agtggcatcc gtaagtccgg
     3721 atgagctgct gaaaacattg cctaagcgaa aagggtaaaa cgccagtttt ctggttactc
     3781 acaactcatt gaatctgcat gatattgcct gccgggtaag gcgtctcgcc gcatccggca
     3841 acaaatgact caacgttgtc ccgcttctgg tttgcgcgat ttttgccagt aaaaatgctc
     3901 gcgcaaacct tccgccgact cttccgccac agcacgcaat tcatcgctgt ccgcttcatg
     3961 acgcagctga tgatccacat tctttaccca cacaaattca tgtcctttgt gccctgccat
     4021 gagttgtcct gagaacagcg cacacgttaa taagacaacc gataacgcct tcgtaaacat
     4081 cctgccacct ttttcttacc ttttgccgct atgatgccga tcgtttcttg aggttattat
     4141 tcagttttgc aaattagcgc aaagaaattc tggaatcttc cttcctgatt ttgcatggca
     4201 ttctgccgtt gcggcgattt agtgctattt tcgagcaaat tacacacgga ggtaaacact
     4261 aatgtttact ttgttgatat actcagcggc agggaggcga tgtgaaacaa agcgagttca
     4321 gacgttggct cgaatctcag ggcgtcgatg tagcgaatgg cagcaaccat ttgaaactca
     4381 ggtttcatgg gaggcgcagt gtcatgccgc gtcacccctg cgatgagatt aaagaaccat
     4441 tgcgtaaagc aatcctgaaa caactcggtt tgagttaatc gccaattaaa aaggttaatg
     4501 acatgcgagg gacagtcgaa attatgcgtt atcccgtcac tcttacaccc gcgccggaag
     4561 gcggttatat ggtttctttt gtggatatcc ctgaagcgtt gacgcagggc gaaactgtcg
     4621 ctgaagcgat ggaagcggca aaagatgctt tactgaccgc atttgatttt tattttgaag
     4681 ataacgagct tatcccgtta ccttcgccat taaatagtca tgatcacttt attgaagtac
     4741 ctttgagcgt cgcctctaaa gtattgctgt taaatgcttt tttacagtca gaaatcactc
     4801 agcaagagtt agccaggcga attggcaaac ctaaacagga gattactcgc ctatttaact
     4861 tgcaccatgc gacaaaaatc gacgccgtcc agcttgcggc aaaggcgctt ggcaaagagt
     4921 tatcgctggt gatggtttaa ttacagttaa cgaaaagttg tcatttttaa caactgatat
     4981 agactgccga atcatctgca cataattacg attcgataat gaaaaaatac cagcagcttg
     5041 cagaacaatt acgcgagcag attgcgtcgg gtatctggca acccggcgat cgtttgcctt
     5101 cgttgcgtga ccaggtggcg ctttcaggca tgagctttat gactgtcagc catgcctatc
     5161 agttgctcga aagtcaggga tatattatcg cacgaccgca gtcgggttat tacgttgcgc
     5221 cacaggcaat aaaaatgccg aaagcgccag tcattccagt cactcgagat gaagcagtcg
     5281 atatcaacac ttatattttt gatatgttgc aggccagtcg cgatccgtcg gtcgttccgt
     5341 ttgcctcggc ctttcccgac ccgcgacttt tccccctcca acaactaaac cgctcgctgg
     5401 cgcaggtaag caaaaccgcc acggcgatga gcgtgattga aaacttaccg ccaggaaacg
     5461 cagaactgcg tcaggctatt gctcgtcgct atgccttaca gggcatcacc atttctcctg
     5521 atgaaattgt cattactgcc ggggcgttag aggcattaaa cctcagtttg caagcggtaa
     5581 ctgaaccggg cgattgggtg atagtagaga atccttgttt ctatggcgcg ttgcaggcgc
     5641 tggaacggct acggctgaaa gcgttatcgg tggcgacgga tgttaaagaa gggatagatc
     5701 ttcaggcgtt ggaactggcg ttgcaggagt atccggtgaa agcgtgctgg ctgatgacta
     5761 atagccagaa tccactcgga tttaccttaa cgccgcaaaa aaaagcacaa ctggtggcgt
     5821 tactcaatca gtacaacgta acgctgattg aagatgacgt ttacagcgaa ctttattttg
     5881 gacgggaaaa accgctgcct gcgaaagcgt gggatcgcca cgatggcgtt ttgcattgct
     5941 cttcgttttc gaaatgtctg gtgcctggtt ttcgtattgg ttgggtcgcc gccggaaaac
     6001 atgcacgtaa aattcaacgc ttgcagttga tgagtacgct ttccaccagc tcaccgatgc
     6061 agcttgcgct ggtggattac ctttccacgc gccgatacga cgcccatctt cgtcgcctgc
     6121 gtcgccagct tgcggaacgt aaacaacgtg cctggcaggc actgctgcgt tatctgcctg
     6181 cggaagttaa aattcatcat agtgacagcg gttattttct ctggctggag ctccccgagc
     6241 cgttagatgc cggcgaatta agcctggcgg cactgacgca tcatatcagt attgcgccgg
     6301 gtaaaatgtt ttctaccggt gaaaactggt cacgtttttt tcgttttaat accgcgtggc
     6361 agtggggaga acgtgaagaa caggcggtaa aacaattagg caaacttatt caagaacggc
     6421 tgtaatagcg tttaatttaa ttcctcttag attgggtaat atgaatttcg aatagcagtc
     6481 atatttccta accccttgtc tatactccag aagataacct tacagacggc ataatgcgcg
     6541 gtagctcaca acctgattaa attttctcag gggcgaaggt gtgcctgcaa gccgccgtct
     6601 atggttaaac aaggagatat ttttacggca cggcggctga acaattaatt acgacaggag
     6661 taagacctta tgagcaagac atttgcccgc agcagcctgt gtgcgctcac catgacaata
     6721 atgaccgctc acgccgccga accacctacc aatttagata aaccggaagg gcgactggat
     6781 attatcgcct ggccgggata catcgaacgc ggacaaactg ataaacaata cgactgggta
     6841 acgcaatttg aaaaagagac aggctgcgcg gtgaatgtga aaaccgccgc gacttccgat
     6901 gaaatggtca gtctgatgac caaagggggt tacgatctgg ttacggcatc cggcgatgcc
     6961 tcgctgcgtt tgattatggg caaacgcgtg cagccgatta ataccgcctt gattcccaac
     7021 tggaaaacgc tcgatccgcg cgtggttaaa ggcgactggt ttaatgttgg cggcaaagtt
     7081 tacggcacac cttaccaatg ggggccgaac ctgctgatgt acaacactaa aaccttcccg
     7141 acgccgccgg atagctggca agtggttttt gttgagcaaa atctgccgga cggcaagagc
     7201 aataaaggcc gcgttcaggc ttatgatggc cctatctaca ttgcggacgc tgcgttgttc
     7261 gttaaagcca ctcagccgca gttgggcatc agcgatccgt atcaactcac cgaagaacag
     7321 taccaggcgg tgctgaaagt gctgcgcgct caacacagtt tgatccatcg ctactggcat
     7381 gacactaccg tgcaaatgag cgatttcaaa aacgagggtg tggttgcttc cagtgcctgg
     7441 ccctatcagg ccaacgccct gaaagccgaa ggccagcctg ttgctaccgt tttcccgaag
     7501 gagggggtta ccggttgggc tgataccacc atgctgcata gcgaagcgaa acatccggtt
     7561 tgcgcctaca aatggatgaa ctggtcatta acgccaaaag tgcagggcga tgtggcggcc
     7621 tggtttggct cgttaccggt agtgccggaa gggtgtaaag ccagtccgtt attaggcgaa
     7681 aaaggttgtg aaaccaacgg ttttaactat ttcgacaaaa tcgccttctg gaaaacgcct
     7741 atagcagaag ggggcaagtt tgttccctac agtcgctgga cgcaggatta cattgccatt
     7801 atgggcggtc gctaacttcg ctggggtgct ttatgacgta cgcagtggag tttgacaacg
     7861 tctcgcggtt gtacggtgac gtgcgcgcag tagatggcgt cagtattgcg ataaaagatg
     7921 gtgagttctt ctctatgctg gggccgtccg gctccggcaa aaccacctgc ctgcgcctga
     7981 ttgctggctt cgaacagctt tccggcgggg ctatctctat ctttggtaaa cccgccagca
     8041 atctgccacc gtgggagcgg gacgtgaata ctgtctttca ggactacgcg ctatttccgc
     8101 atatgtcgat tcttgacaat gtcgcctatg ggctgatggt caaaggcgtg aataaaaagc
     8161 agcggcacgc aatggcgcaa gaggcgctgg agaaagtggc gttggggttt gtacatcaac
     8221 gtaaaccgtc acaactttct ggtggtcagc gccagcgggt tgctatcgcc agagcattgg
     8281 tgaatgaacc gcgcgtattg ctgttggatg aaccgctcgg cgcactggat ctcaaattgc
     8341 gtgagcagat gcagctggaa ctgaaaaaac tgcaacagtc tctcggtatc acttttatct
     8401 tcgttaccca cgatcagggc gaagcgttat cgatgtccga tcgtgtggcg gttttcaata
     8461 atggacgcat tgagcaggtc gattccccgc gcgatctcta tatgcgcccg cgcacgccgt
     8521 ttgttgccgg attcgtcggg acatccaacg tttttgatgg actgatggca gagaaacttt
     8581 gtggcatgac gggaagcttc gccctgcgac cggaacatat ccgcctcaac acccctggtg
     8641 aactgcaggc caatggcacg atccaggcgg tgcaatatca gggcgcggca actcgttttg
     8701 aactgaaatt gaacggcggt gaaaaactgc ttgtgagtca ggccaatatg acaggcgaag
     8761 aactgcctgc cacgctcacg cccggacaac aggtgatggt ttcctggtcg cgtgatgtca
     8821 tggtgccgct ggttgaggag aggtgaatgg cgatgaatgt attgcaatca ccttcacgtc
     8881 caggtctggg taaggtgtcc ggtttcttct ggcgtaatcc ggggctgggg ctgtttttac
     8941 tgctgcttgg cccgctaatg tggtttggca ttgtttattt cggctcgctg ctaacgctgt
     9001 tatggcaggg cttttatacc tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     9061 tggcgaatat tcgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     9121 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     9181 tggcgcgcta taccagcggg aaaatgaaag cgttttttta tattgcggta atgttgccga
     9241 tgtgggcgag ctacattgtt aaagcctatg cctggacgtt attgctggca aaagatggcg
     9301 tggctcagtg gtttttacaa catcttgggc tggaaccact gctgactacg ttccttacat
     9361 tacctgcggt gggcggcaat acgctgtcaa cttccgggct ggggcgcttt ctggtgtttc
     9421 tctatatctg gctgccgttc atgatcctgc ccgtgcaggc ggcgcttgag cgtttgccgc
     9481 cgtcattgtt gcaggcgtcg gctgatctcg gcgcacgtcc acgacaaacc tttcgctatg
     9541 tggtgctgcc gctggcaatc ccgggtattg ccgctggctc tatctttacc ttctcactca
     9601 cactgggcga ttttatcgtc ccgcagctgg ttggtcctcc aggatatttt atcggcaata
     9661 tggtttattc ccagcagggg gcgattggca atatgccgat ggcggcggca ttcaccctgg
     9721 tgccgattgt tctcatcgca ctgtacctgg cgttcgtgaa acgtctggga gcgttcgatg
     9781 cactctgaac gcgcaccgtt tttcctcaaa ctggcggcct ggggcggcgt tgttttccta
     9841 cattttccca tcctgataat cgccgcctat gcgtttaaca ctgaagatgc ggcgtttagt
     9901 tttccaccgc agggcctgac gctgcgctgg tttagcgtgg cagcacggcg tagtgatatt
     9961 cttgatgccg tgacactgtc acttaaagtg gcggcgctgg cgacattaat tgcgctggtg
    10021 ttagggacgc tggcagctgc cgcgctgtgg cgacgagact ttttcggcaa aaacgccatt
    10081 tcgctgttac tgctgctgcc cattgcgctg ccgggcattg tcactggtct ggcgctatta
    10141 accgctttta aaaccatcaa tctggagccg ggatttttca ccatcgtggt cggtcatgcg
    10201 actttttgcg tagtggtggt gtttaacaat gtcatcgccc gttttcgccg cacctcgtgg
    10261 agtctggttg aggcgtcaat ggatcttggg gccaatggct ggcaaacctt ccgctacgta
    10321 gtgttgccga atctcagttc ggcgctactg gcaggaggaa tgctggcgtt tgccttgtcg
    10381 ttcgatgaaa tcatcgttac gacttttacg gcaggtcatg aacgaacgtt accgttgtgg
    10441 ttgctcaatc agcttgggcg accgcgtgat gtaccggtaa ctaacgtggt ggcactgctg
    10501 gttatgttgg taacaacctt gccgatcctg ggggcctggt ggctaacccg cgaaggcgac
    10561 aatggtcaat aaccactgat acaggaatat gctatgcaac ataagttact gattaacgga
    10621 gaactggtta gcggcgaagg ggaaaaacag cctgtctata atccggcaac gggggacgtt
    10681 ttactggaga ttgccgaggc atccgcagag caggtcgatg ctgctgtgcg cgcggcagat
    10741 gcagcatttg ccgaatgggg gcaaaccacg ccgaaagtgc gtgcggaatg tctgctgaaa
    10801 ctggctgatg ttatcgaaga aaatggtcag gtttttgccg aactggagtc ccgtaattgt
    10861 ggcaaaccgc tgcatagtgc gttcaatgat gaaatcccgg cgattgtcga tgtttttcgc
    10921 tttttcgcgg gtgcggcgcg ctgtctgaat ggtctggcgg caggtgaata tcttgaaggt
    10981 catacttcga tgatccgtcg cgatccgttg ggggtcgtgg cttctatcgc accgtggaat
    11041 tatccgctga tgatggcagc gtggaaactg gctccggcgc tggcggcagg gaactgcgta
    11101 gtgcttaaac catcagaaat taccccgctg accgcgttga agttggcaga gctggcgaaa
    11161 gatatcttcc cggcaggcgt gattaacgta ctgtttggca gaggcaaaac ggtgggtgat
    11221 ccgctgaccg gtcatcccaa agtgcggatg gtgtcgctga cgggctctat cgccaccggc
    11281 gagcacatca tcagccatac cgcgtcgtcc attaagcgta ctcatatgga acttggtggc
    11341 aaagcgccag tgattgtttt tgatgatgcg gatattgaag cagtggtcga aggtgtacgt
    11401 acatttggct attacaatgc tggacaggat tgtactgcgg cttgtcggat ctacgcgcaa
    11461 aaaggcattt acgatacgct ggtggaaaaa ctgggtgctg cggtggcaac gttaaaatct
    11521 ggtgcgccgg atgacgagtc tacggagctt ggacctttaa gctcgctggc gcatctcgaa
    11581 cgcgtcagca aggcagtaga agaggcgaaa gcgacagggc acatcaaagt gatcactggc
    11641 ggtgaaaagc gcaagggtaa tggctattac tatgcaccga cgctgctggc tggcgcatta
    11701 caggacgatg ccatcgtgca aaaagaggta tttggtccag tagtgagtgt tacgcccttc
    11761 gacaacgaag aacaggtggt gaactgggcg aatgacagcc agtacggact tgcatcttcg
    11821 gtatggacga aagatgtggg cagggcgcat cgcgtcagcg cacggctgca atatggttgt
    11881 acctgggtca atacccattt catgctggta agtgaaatgc cgcacggtgg acagaaactt
    11941 tctggttacg gcaaggatat gtcactttat gggctggagg attacaccgt cgtccgccac
    12001 gtcatggtta aacattaagg ataatattgc agatcgtaag agtatgagat gatcttgcgt
    12061 actgtcagag agttgatttc tctgacagta cgtgctgcgt gcaaaatcac tacatcaaat
    12121 aaaccagcca aatctcatag agataagagt aaacaaacca acagcagcaa agacatttat
    12181 cagtactaca gttctactgg aaacattcat ttttgccacc ttttatcatt gcccctatgt
    12241 gtatagtacg ggttaagaaa atccgcaata atactcattc tgaagatgga taagggcaag
    12301 ttgctgtttg atgatttttt ctcaaactgg tgaaaaaact tgatgcacgt caaaaaatga
    12361 cgcatatttg cgcgcgtttt attcatctgg ctggacgccc gtacatgtct ctctatcaac
    12421 atatgcttgt tttttatgcg gttatggcag caatcgcatt tcttatcacc tggtttcttt
    12481 ctcacgataa gaaacgcatc cgtttcttaa gcgcctttct ggtgggggca acatggccga
    12541 tgagtttccc ggtggcgctg ttgttttcac tgttttaaat cttactcact ggcttgacgg
    12601 caccacgcag acttatatca tttggatgaa tcgataaatt tcacaagtgg ttaaggagaa
    12661 agtatgtcgc atctggatga agtcatcgcg cgcgtggatg ccgcgattga agagagcgtc
    12721 attgcccata tgaacgaatt attaatcgcc ctgagcgatg acgcggagtt aagtcgggaa
    12781 gatcgctaca cccagcagca gcgtttgcgc accgctattg ctcatcacgg tcgcaagcat
    12841 aaagaagata tggaagcgcg ccacgaacag ttaaccaaag gcggcaccat cctctgatta
    12901 aaatgagcgt ctggctacca accaggcgcc aaccacaagt aaaattgcgc cacaaattgg
    12961 gccgaacaac gcccgtagtg gtacgccatg actgcggaag atatccagca tcagaccgcc
    13021 aattagctgg ctggcgacca gtactgcaat agttgtcgcc gctcccacat tctgatatcc
    13081 gctgatactg gcgaagacaa aaaacgatcc cagcaagccg ggtataagtg tccaccagcg
    13141 cacgctggac accagttcgc caaaccccgc catgccttgt ttaaaccata aaatagaaac
    13201 aaagagcaca atccccacca gtgagttcag caacatggcg atgagaatgg tagaggaggt
    13261 ctgggtgatg cgcaccatca gcgtgttctg gactaccaga ccaattccgg cggcaatcaa
    13321 aaaagcaagg gtgagcgact gattcatcca atcgcgtccg gttcagtgcg ttcgtcgagt
    13381 tgcaactgca taaatgtcag atccagccag cgcccaaatt tagtgcccac ctgcggcatt
    13441 tgtgcggtga cgacaaatcc cagcgaatgg tgcagatgca gcgaggcctg attttgcgat
    13501 tcgatcccgg cgaccatgac atgcttcccg caatcccgcg cttcatcaat caatcggctt
    13561 aacaatttac gccccagacc tttgccctga tgatcgggat ggacataaac cgaatgttcc
    13621 acggtatggc gaaaaccatc gaaactacgc cagtcgccaa acgaggcata tcccgtcact
    13681 acgccatctt cttcactcac cagcaccgga taacctgcta tggtccgcgc ttcaaaccag
    13741 gcaatgcggt tatctgcatc aaccgtttgg tcattccaga tagccgccgt atacaacacg
    13801 gcgtggttat aaatttccgc aatggcagca caatcggctt tgcgggcaaa acggatggac
    13861 atgtctggcc tcgataaaat aatgatgatg atgttatcaa tactatcgaa aacagcccga
    13921 agcaacggat tcctcgatga aattattgcg tgttgcagag gggatgtgac ggctgcaaac
    13981 aaaactaatc ccgtgagcct gctactattg atggattcat gccaaaagca caggagagca
    14041 ttatggggca acaaaagcag cgtaatcgac gttgggttct ggcctcgcgt ccacatggcg
    14101 caccggttcc ggagaatttc cgtcttgaag aagatgatgt cgccacaccg ggtgaaggac
    14161 aggtgttact gcgcacagtt tatttgtccc tggacccgta tatgcgtgga cgtatgagcg
    14221 atgagccatc ttattcaccg cctgttgata ttggcggcgt gatggtcggc ggcacggtga
    14281 gccgtgttgt ggagtcgaat catcctgatt atcagcctgg cgactgggtg ctgggctaca
    14341 gtggatggca ggactatgac atatccagtg gtgatgatct ggtgaaactt ggcgatcatc
    14401 cgcaaaatcc atcgtggtcg ctgggtgtgc tggggatgcc aggctttacc gcttatatgg
    14461 ggctgctgga tatcggtcag cctaaagagg gcgaaacgtt ggtggtagct gcggcgacag
    14521 gtcctgtggg agcaacggtg gggcaaatcg gcaaacttaa aggttgcagg gtggtggggg
    14581 ttgccggtgg cgcggaaaaa tgccgccatg ctatcgaggt gctgggcttc gatgtttgtc
    14641 ttgatcacca cgcggatgat tttgccgaac aactggtgaa agcgtgccca aaaggtattg
    14701 atatctatta tgaaaacgtg ggcggtaagg tatttgatgc agtgctacca ttacttaata
    14761 catctgcgcg cattcccgtt tgtggcttag tgagcagcta taacgctaca gagctaccac
    14821 ccggtccgga tcgtttaccc ctgttgatgg caacggtgct gaaaaaacgt attcgcttgc
    14881 aagggtttat tatcgctcag gattatggtc accgcatcca tgagtttcag aaggagatgg
    14941 ggcaatgggt gaaagaggat aaaatccact accacgaaga tattactgat ggtttagaga
    15001 atgcgccaca gacgtttatc ggcctgctga agggtaaaaa cttcggcaaa gtggtgatcc
    15061 gcgtcgcggg tgatgattaa agtttcaacg gcggcgtaag ccgccataaa aatccaggca
    15121 aaaagcttat tttcagcttt aattaactaa ccacgtcatt gttctgctgc taataacaac
    15181 aaaggtgggt ggtttatgat tattcacttt aatacaccag gtgaactcct tctgccgtgc
    15241 aggcagggtt ggacagaaaa cacgcgaagg aattaaaatg cctggaatgg gaaaaatgaa
    15301 acatgtcagt ttgactctgc aggttgagaa cgacctgaaa catcagctta gtattggcgc
    15361 attaaaacct ggcgcacgcc tgattactaa aaatctggcg gagcaattag gtatgagtat
    15421 tacacctgtg cgtgaagcat tattacgtct ggtttcggtg aatgcgcttt ctgtcgcacc
    15481 tgcacaagca tttacagttc cggaagtgag gaaacgtcaa ttagacgaaa tcaatcggat
    15541 ccgctacgaa ctggaattaa tggcagttgc tctggctgtt gaaaacctca ccccgcaaga
    15601 cctggcggaa ctccaggaat tgctcgagaa attacagcag gcgcaagaaa agggcgacat
    15661 ggaacaaatc attaatgtaa acaggctatt tcgcttagcg atttatcatc gctcaaatat
    15721 gcccatcctg tgtgagatga ttgagcaact gtgggtcagg atggggcctg gtttacatta
    15781 tctctatgaa gcgattaatc cagcggaatt acgggagcat atagaaaact atcatctgtt
    15841 actcgctgcg ttaaaagcaa aagacaaaga gggatgcaga cattgtcttg ctgaaattat
    15901 gcaacaaaat attgctattt tataccagca atacaatcgt taaagtaaat gcccggcaaa
    15961 taccgggcat caaggtaatt actcaaatct ccacgcaata ttcatgccga caccataatt
    16021 tcgtccgggc gaaggttcgt aatatcgccc gtttgactca ttgacaatga cagaaccaac
    16081 gtattcttta tcgaataaat tatcgacacg accaaataaa tccacagtta aattgtggta
    16141 attgtattta tacccggtga ataagccgac gagagtataa gacggcgctt ttgccgtatt
    16201 ttcatcatct gccataatat cgcccatata acgcgcttcc gtgcctgcat accaaccatc
    16261 ttccggtaca taacctatcg acgcaaagcc catattacgg gcgatccctg gcatccgatt
    16321 accgttacaa tcctgttcat tgcaaacatt gctgcgatag gtcgcatcaa gccaggtcca
    16381 ggacgcgttt acgcgaaaat ctcctgcgaa acgttgatcc catgccagtt cagcgccttg
    16441 acgacgggtc tttccggcat ttttgtaagt cgtacgccca ccgctactgc tatcgacaac
    16501 aatttcatca tcagtgtcgg tctgaaacaa tgcgagacta agcagcccat caccaatacg
    16561 cgttttactg ccgatctcaa ttgtatcgtt ggtggatggt tttaaaccaa agttcatacc
    16621 gctttgccca tcagcacgat aagacagctc attaatcgtc ggcgtttcaa aacctcgccc
    16681 ggctgccaga tagatattcc aggcatcggt cattgcatat tttaacgaac cggcaggtag
    16741 ccatttatga taactggcat caccgctgtc atcgccgtta cccggagtaa cgtaatggtc
    16801 gttggaatca aaccacacgg agctgtagcg cacgccagca tccagcgaca gtttttcgct
    16861 cagctgccac tgcgtctgta aatagggatc gatgttccac atcagattgc gttcgtcgcg
    16921 acgcaactca cctttttgcc cgtactccgg catgccgcta ttcaggcgga agttattgta
    16981 gcccttgcgg ttttcactca tgttttcgta gttcaggccg gtagtgaacg tgaccggaac
    17041 gcccagttca ccacggtgtg tccagcggct gtctatcccc tggtaatggc gttgcagggt
    17101 aatcacgccg cccgcatgtg acgggttaag ttggggggcc atcgggattg actggtactg
    17161 ggtcgtttct cgctctccgg catacatcat cacactcata tcatcttgcg cgctcaggct
    17221 acgctcatag cgcaacccag cctgagtttg cttgatggtt tttcgcgtgt cgtactgttc
    17281 tgcacgaggc gcttgttgtg gattagcctt ccattctgct ttggttagcc cacctgggtc
    17341 atctgctttg atatccacac tattgaaaat cagacttaat ttgctggctt catcaatgcg
    17401 tacgcccagt ttggcattag ctaaattttt ctgtgcgcca ctatggtcac gatagccgtg
    17461 ggtcgtaaaa cgcgtggttg agacggtgta atcgacatcg ccaggctgtg tgccgtctcc
    17521 cgttgcgccc gttgctttca gcccatagcg ccagctgcca aaactgccgt agtaactact
    17581 ggcttcaatg gttggtggct gttgtccggt ctgggtggtg acattcatta ccccaccaga
    17641 cgcgttgcca tacagggcag agaaggggcc acgcagcact tccacatttt gcacactgct
    17701 taaatcgatg ttggatgttt gcccttgccc gtcgggcatg gtggcgggaa taccgtccac
    17761 atacaggcga ataccgcgaa taccgtaagt ggagcgggag ccaaatccgc gaatcgacag
    17821 ctgtaaatct tgcgcatagt tctgccggtt ttgtacctgc aaaccaggca cgccggtcag
    17881 tgattcggac aagttaatgc gcggtgttgc caggcgcatc tcctcgccat ccaccacgct
    17941 tactgctgct ggggtatcca gttctgaaac cacctgcggt gcggcactga caatcatagt
    18001 ctgttcatca gcggcaaaaa caacggggga aaggacaagc agtgcgggca aaacggtctg
    18061 tcggacggaa aaaatcttca tgaaaaaagc caggttaaga atgggaaaac gccgttatgg
    18121 taatgaaatt gtaaatttat ggaaaacgaa acggcacgat acgttaagta attgagaaaa
    18181 ttgtagtcgt aacggcaaga aatgctccac atttgagaaa ataatgatta ccattcccat
    18241 ttataacaag agcgtaacga tgattacgct tagcgaagca ttgtgaagca gcaaaaatat
    18301 cggttcatca aagggagtcg tcatgcattt acgtcatctg ttttcatcgc gcctgcgtgg
    18361 ttcattactg ttaggttcat tgcttgttgc ttcatcattc agtacgcagg ccgcagaaga
    18421 aatgctgcgt aaagcggtag gtaaaggtgc ctacgaaatg gcttatagcc agcaagaaaa
    18481 cgcactgtgg ctcgccactt cgcaaagccg caaactggat aaaggtggcg tggtttatcg
    18541 tcttgatccg gtcactctgg aagtgacgca ggcgatccat aacgatctca agccgtttgg
    18601 tgccaccatc aataacacga ctcagacgtt gtggtttggt aacaccgtaa acagcgcggt
    18661 cacggcgata gatgccaaaa cgggcgaggt gaaaggccgt ctggtgctgg atgatcgtaa
    18721 gcgcacggaa gaggtgcgcc cgctgcaacc gcgtgagctg gtagctgatg atgcctcgaa
    18781 caccgtttac atcagtggta ttggtaaaga gagcgtgatt tgggtcgttg atggcgagaa
    18841 tatcaaactg aaaaccgcca tccagaacac cggtaaaatg agtaccggtc tggcgctgga
    18901 tagcaaaggc aaacgtcttt acaccactaa cgctgacggc gaattgatta ccatcgacac
    18961 cgccgacaat aaaatcctca gccgtaaaaa gctgctggat gacggcaaag agcacttctt
    19021 tatcaacatt agccttgata ccgccaggca gcgtgcattt atcaccgatt ctaaagccgc
    19081 agaagtgtta gtggtcgata cccgtaatgg caatattctg gcgaaggttg cggcaccaga
    19141 atcactggct gtgctgttta acccagcgcg taatgaagcc tacgtaacgc atcgtcaggc
    19201 aggtaaagtc agtgtgattg acgcgaaaag ctataaagtg gtgaaaacgt tcgatacgcc
    19261 tactcatcca aacagcctgg cgctgtctgc cgatggcaaa acgctgtatg tcagtgtgaa
    19321 acaaaaatcc actaaacagc aggaagctac ccagccggac gatgtgattc gtattgcgct
    19381 gtaatagatg tggcgagggt gaaaacggag agacttgcct gacttttatt acgccgcatg
    19441 cggcacacct tcgtcagtaa tctaaatttg cccggcacgt agccgggcaa ttttgcaatt
    19501 agctcgccgt ttcaggctta aacacaattt cctgtttttc ttcatcttcc tcgacgactg
    19561 gcgcagtgct gtgaatttca gcaacgcgtt tgcgcacacc aaaccagcct ataaccagca
    19621 gaataccgat aatcggcagc gccgcgatag tataagtccc gttcgggtaa tcgaacgcca
    19681 tcagcaccag gacactcagt aaaaacagta atgtcagcca ggaagtgaag ggcgcgccag
    19741 gcagtttaaa actgacatcc gctgctttgc cttgtttaat tgctttacgc aggcgcatct
    19801 ggcacacgat gataaacgcc catgaagcga tgattcccag cgacgcgaag ttcaacacaa
    19861 tctcaaatac gcgcgacggc accagatagt tgaggaatac gccgacgaca tacacaacta
    19921 gtgtcgccag aatcccggca tacggcacat gctgacgact cattttcgcc ataaaactcg
    19981 gtgcggaacc gcccatcgcc attgagcgca gaatacgtcc ggtgcagtac agacctgagt
    20041 tcaggctgga gagggcagcg gtcagcacca caatgttcat aatgctgccg atatatggca
    20101 cacccagttt agagaaaaac gtcacgaacg gactttgccc cgcctgatac gcgctccacg
    20161 gcaatagcat aaccagcaac accacggagc cgacgtaaaa caggccaata cgccaaatca
    20221 cgctgttaat cgctttaggc accatggtct gcggatcttt acattctccg gcagctgtac
    20281 ccaccatttc aatggaggca aaagcaaaca ctacgccctg aatcaacacc agagcaggca
    20341 gcaaaccgtg ggggaagaag ccgccattat cggtaattaa atgaaagcca gtggtgttgc
    20401 catccagcgg ctgaccacta ccgaggaaca ctgtacccac gaccaaaaag gtcacaatgg
    20461 cgagcacttt aataagcgca aaccagaact ccatctccgc aaaccatttc acaccgatca
    20521 tattcatggt gccaacgatg gtaagtgcag cgagcgcaaa gacccactgc ggcacgccgc
    20581 caaacgcacc ccagtaatgc atatacagag cgacggcggt aatatcaaca atccccgtca
    20641 tcgcccagtt gatgaagtac atccagccag caacataagc ggctttctca cccaaaaact
    20701 cacgggcata agagacaaaa ctgccactgg aagggcggtg tagcaccagc tcacccaatg
    20761 cacgcagaat aaaaaacgaa aacaagccac aaattaaata aaccagtgcc aatgctggac
    20821 ccgccatttg cagtcgggct cctgcaccta aaaacaagcc ggtgccaatc gcgccgccaa
    20881 tggcgatcat ctgtacctgg cgattgccca tcgctttgtg atacccctct tcgtgggcat
    20941 taagccagcg gcgtttcgcg gcgtgttgat ctgaagtgtc ggtgtcgtgt ttactcattg
    21001 ctctccctga ttgctttaat gaaaaagtca tataagttgc catgaacaat gtttattcat
    21061 cctggcgatg atagttatca ccattattct ttatgggtat aaaactatcg gcgcagcatc
    21121 ctacccgtta tcgataaacg atgcaaaaca tccccttaca atcctgaagg ggaataatac
    21181 aactgacgaa aaaatgacaa atctttttgc tggttaacct gtgtactgtc ctacacttaa
    21241 tctttaaaag attgtgaggg catatgatta aggtgtatgg cgtacccgga tggggctcta
    21301 caatcagtga gctgatgcta acactggctg atattcctta tcagttcgtt gatgtcagtg
    21361 gttttgacca cgagggagct tcacgcgagt tattgaaaac cctgaacccg ttgtgtcagg
    21421 ttccgacact ggcgctggaa aatgacgaga ttatgacgga gacagcggcg attgcattta
    21481 tggttctcga tcgtcgcccg gaccttgcgc caccggttgg gcgcgccgaa cgtcagcagt
    21541 ttcaacggct attggtctgg ctggttgcta atgtctatcc aacattcact ttcgccgatt
    21601 accctgagcg ttgggcatct gacgcacctg aacagttaaa gaagaatgta attgaatatc
    21661 gcaaatcact ttatatctgg ctgaattcac aattgaccgc tgaaccttat gcctttggtg
    21721 agcaactaac gctggtggac tgttatctct gcaccatgcg cacatggggg ccggggcatg
    21781 aatggtttca ggataatgcg cagaatatca gcgcgatcgc tgatgctgta tgtcagttac
    21841 cgaaattaca agaagtatta aaaaggaatg aaattatttg agtgggatac cagaggatta
    21901 tgttatttat tattcagtaa ctttatccac aatgatgggc gtaattaatt aaattcatgt
    21961 tatgtttttt gatttatatc actctcccct tcattcagct tgtctatttc atttcctctg
    22021 aaaagaaact aactattcac attgtgcaga tgtttcactt gttatcacag gtattctata
    22081 atctcaaaaa gtttttaatg atggatatgc tcggagttgg agatgcaatt aatattaata
    22141 caaataaaaa tatccggcag gtatgctaaa aggataaatg gcgttctctg tatttattcg
    22201 caattatctg taggacaaat atggagagct tgatgagtca gagctaaaac agcaattgat
    22261 aaataaacat gttgatttat tcaagaaaat ggatgtattt taaatattga ttcttaatat
    22321 aacctaatca atagattatc acgtgagagc tgcacattat tggcggggcc ggggaaaaca
    22381 tcattttggg aacaatgaat caggtactct taccactgaa gcaagatcat ccgggcacct
    22441 ggtggtggtg cagtgctttg gaaatggtcc cctcggggga attgtcatta ttttctatgc
    22501 tatttgctta tgatattcat caggtgaatt taataaattc ctaatttgaa atatccacta
    22561 ttaagccagt gtttaacgac gttaagatgg caatgtgcag atcatgttat cagggtgggg
    22621 cggtagcgta tattcctaat acaaatcatc attcatttca atgattgtta ttgaattatt
    22681 tattgttgtg aataaaaaga cattatgaat attggtttga ctttgcatat caggtttttt
    22741 attactcgct gtaatgtacg agcctgtcgt gaataacgtc gagaacccat ccgttgcgtt
    22801 tgtgattatt ttgttgacta aacagacacc cgtttctctg aagtaaaatc ccagactaaa
    22861 tcatcacata accatgacat ttttctgata ttccccggta acgccagatg tcgactcgct
    22921 tatctcatcc gcccgacatg ttgtacatgc aatatgtgcg gattattatt cattttcacg
    22981 gaggttgcta tgtcaaccgg attacgtttc acacttgaag tggacggcct gccaccggac
    23041 gcgtttgcgg tggtttcctt tcatctcacc cagtcactct cttcactttt ttccctcgac
    23101 ctttctctgg tcagtcagca gtttctttcc cttgaatttg cgcaggtgct ggacaaaatg
    23161 gcctacctga cggtatggca gggcgatgac gtacagcgcc gggtgaaagg tgtggtgacc
    23221 tggtttgaac tgggggagaa cgacaaaaac cagatgctgt acagcatgaa ggtgtgcccg
    23281 ccgctgtggc gcacagggct gcgccagaac ttccgtatct tccagaatga ggacatcgaa
    23341 agcatcctcg ctacgatcct gaaggaaaac ggtgtgaccg agtggagtcc gctgttcagc
    23401 gagccgcatc cttcccgtga gttttgtgtc cagtacggcg aaaccgatta cgatttcctg
    23461 tgccggatgg cggcggagga aggcatcttc ttttatgagg agcacgcgca aaaagtaccg
    23521 accagagcct ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt gagatcccct
    23581 ggaatccgaa cacccgtacc gaggtgagca ccctctgcat cagccagttc cgctacagcg
    23641 cacaaatccg cccttcttcc gtggtgacca aggactacac ctttaaacga cccggctggg
    23701 cagggcgttt tgatcaggaa ggccagtacc aggattacca gcgcacgcag tatgaagtgt
    23761 atgactaccc cggacgtttc aagggtgccc acgggcagaa ctttgcccgc tggcagatgg
    23821 acggctggcg aaacaatgca gaaaccgcgc ggggaatgag tcgctcgccg gagatatggc
    23881 cgggacg
//
LOCUS       DAFATA010000065        23736 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFATA010000065 DAFATA010000000
VERSION     DAFATA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.066"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..125)
                     /locus_tag="LGJ20_003080"
     CDS             complement(<1..125)
                     /locus_tag="LGJ20_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBK2387107.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGD"
     gene            complement(231..1733)
                     /gene="dtpA"
                     /locus_tag="LGJ20_003081"
     CDS             complement(231..1733)
                     /gene="dtpA"
                     /locus_tag="LGJ20_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBK2387108.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFESINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2344..2979)
                     /gene="nth"
                     /locus_tag="LGJ20_003082"
     CDS             complement(2344..2979)
                     /gene="nth"
                     /locus_tag="LGJ20_003082"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK2387109.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2979..3674)
                     /gene="rsxE"
                     /locus_tag="LGJ20_003083"
     CDS             complement(2979..3674)
                     /gene="rsxE"
                     /locus_tag="LGJ20_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBK2387110.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            complement(3678..4298)
                     /gene="rsxG"
                     /locus_tag="LGJ20_003084"
     CDS             complement(3678..4298)
                     /gene="rsxG"
                     /locus_tag="LGJ20_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBK2387111.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQEA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4302..5360)
                     /gene="rsxD"
                     /locus_tag="LGJ20_003085"
     CDS             complement(4302..5360)
                     /gene="rsxD"
                     /locus_tag="LGJ20_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBK2387112.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5361..7487)
                     /gene="rsxC"
                     /locus_tag="LGJ20_003086"
     CDS             complement(5361..7487)
                     /gene="rsxC"
                     /locus_tag="LGJ20_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBK2387113.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQSNAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(7480..8058)
                     /gene="rsxB"
                     /locus_tag="LGJ20_003087"
     CDS             complement(7480..8058)
                     /gene="rsxB"
                     /locus_tag="LGJ20_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBK2387114.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(8058..8639)
                     /gene="rsxA"
                     /locus_tag="LGJ20_003088"
     CDS             complement(8058..8639)
                     /gene="rsxA"
                     /locus_tag="LGJ20_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBK2387115.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(8716..9156)
                     /locus_tag="LGJ20_003089"
     CDS             complement(8716..9156)
                     /locus_tag="LGJ20_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBK2387116.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(9242..9457)
                     /gene="ydgT"
                     /locus_tag="LGJ20_003090"
     CDS             complement(9242..9457)
                     /gene="ydgT"
                     /locus_tag="LGJ20_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBK2387117.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(9730..9855)
                     /gene="blr"
                     /locus_tag="LGJ20_003091"
     CDS             complement(9730..9855)
                     /gene="blr"
                     /locus_tag="LGJ20_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBK2387118.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            10098..11138
                     /locus_tag="LGJ20_003092"
     CDS             10098..11138
                     /locus_tag="LGJ20_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK2387119.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(11173..12174)
                     /gene="add"
                     /locus_tag="LGJ20_003093"
     CDS             complement(11173..12174)
                     /gene="add"
                     /locus_tag="LGJ20_003093"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK2387120.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAGGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(12278..13450)
                     /gene="malY"
                     /locus_tag="LGJ20_003094"
     CDS             complement(12278..13450)
                     /gene="malY"
                     /locus_tag="LGJ20_003094"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBK2387121.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(13460..15052)
                     /gene="malX"
                     /locus_tag="LGJ20_003095"
     CDS             complement(13460..15052)
                     /gene="malX"
                     /locus_tag="LGJ20_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBK2387122.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGIMPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            15227..16255
                     /gene="malI"
                     /locus_tag="LGJ20_003096"
     CDS             15227..16255
                     /gene="malI"
                     /locus_tag="LGJ20_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBK2387123.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            16367..17134
                     /gene="hdhA"
                     /locus_tag="LGJ20_003097"
     CDS             16367..17134
                     /gene="hdhA"
                     /locus_tag="LGJ20_003097"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBK2387124.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            17363..17953
                     /gene="uidR"
                     /locus_tag="LGJ20_003098"
     CDS             17363..17953
                     /gene="uidR"
                     /locus_tag="LGJ20_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBK2387125.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            18342..20153
                     /gene="uidA"
                     /locus_tag="LGJ20_003099"
     CDS             18342..20153
                     /gene="uidA"
                     /locus_tag="LGJ20_003099"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBK2387126.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            20150..21523
                     /gene="uidB"
                     /locus_tag="LGJ20_003100"
     CDS             20150..21523
                     /gene="uidB"
                     /locus_tag="LGJ20_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBK2387127.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            21562..22827
                     /gene="uidC"
                     /locus_tag="LGJ20_003101"
     CDS             21562..22827
                     /gene="uidC"
                     /locus_tag="LGJ20_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBK2387128.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGGSVQKDVSNQIQSILEKGIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNDTA
                     LVSGQVTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(23053..>23736)
                     /locus_tag="LGJ20_003102"
     CDS             complement(23053..>23736)
                     /locus_tag="LGJ20_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HBK2387129.1"
                     /translation="LDYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQ
                     ALLAQPEIANNPALYQEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLF
                     LKDPATTKEAPQTLAQEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLA
                     KQQVEGASAMGQMFRLTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNV
                     PAVPAIPQQ"
BASE COUNT         6017 a         5885 c         6103 g         5731 t
ORIGIN      
        1 tcgtcaccgt tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt
       61 ccgctctcac gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat
      121 ttcatcggta actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa
      181 aaaagccgct aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca
      241 gcagctttcg ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag tttaggcgcg
      301 gtcagcagca tcagtactgc aataatggca gtagcgacac caatctgcaa gaatacgcga
      361 ccataaactt ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc
      421 gcaacataac caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg
      481 aagcccatca gacgctgcgg aacgagttgt gcaaccattg ccagacctag accagagatc
      541 atcagttccc cgatgctctg caggccatag cttgcgacca gccagcttac agacacgata
      601 ccagcgtcag acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc
      661 accatgccga ttgcaaactt ggttggcatc ggcagggtat cgcccatctt gttatagata
      721 gcggccagaa tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca
      781 ggttctacgg ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt
      841 gacgttggca tctggctgta cagcacgaag aagataatgg cttcgagcat caggatgaag
      901 gcaacgatca ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata
      961 accacgatac cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc
     1021 agccaggtgg cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg
     1081 tagttgatag actcgaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag
     1141 gcgaagttaa cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag
     1201 ccgtatttcg cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg
     1261 gacatgtagt acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta
     1321 gaaagcagag aagacgggtt agctttaaac aggccgttac cgaccgcaat agccgccata
     1381 cccatataaa cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc
     1441 agcacaatag cgccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag
     1501 ccgccgatag cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa
     1561 tccgcttcag acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg
     1621 taataaccaa aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt
     1681 ttgaaagcgt tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc
     1741 tcttttttta catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac
     1801 gcgcaattgt tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag
     1861 acaagtcctt tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg
     1921 aaatgttatc cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt cactgcactg
     1981 agaggggggt gatggcttaa cggcgatatg ttctgctatt tagcgttttt actagtgaga
     2041 tagtccagtt tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt
     2101 gtaaatttaa atgattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg
     2161 atcttgatca cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt
     2221 ggttatccgc aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa
     2281 aagatgtttt aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt
     2341 tcttcagatg tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca
     2401 gcggggcttg cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc
     2461 gactttaaac tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt
     2521 ccccggcgca aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt
     2581 cggccagccg aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag
     2641 ggcttcaagc gcagcacgat cttccggaac ctcgccatta tgctgctcca gcaagatacg
     2701 gcaggttttg atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt
     2761 tttcacccct tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtagag
     2821 tttcgccgtc gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat
     2881 cagcaattca aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg
     2941 caggcgagtg aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg
     3001 tttggcaatg cacgttcggc agttgcttca gcacggcgct ttttcattct ttcatcaatc
     3061 aggtattttc ctgccagcat cagtcccagg ccaataaatg cacctggagg cagcatcgcc
     3121 agcaggaaag gggagtcggt gtggaaaatt tccacgcgta atacttttgc ccagctacct
     3181 aacagcgcat ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc
     3241 agcacgaaca tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc
     3301 gccggacctt ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca
     3361 atcagcggaa taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc
     3421 atctgtacag cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg
     3481 gctggcgtcc agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta
     3541 gtcgcaagtc ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa
     3601 aggccgagca actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct
     3661 ttaatttcgc tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac
     3721 gtctgagcgt acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc
     3781 gcgccggtga actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc
     3841 gcaccactga tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt
     3901 ttatcgccaa gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg
     3961 ttaaaatcgg ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct
     4021 tccagaacgg cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct
     4081 ttacctaact ctggcgcagt taccagatag caactctgtg ccagcgcatt gttatagcgt
     4141 tcggctggca gcacctgatc aaataatgcc tcttgttgca gactggcctg ttcagcaatc
     4201 gtcgttttgg tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc
     4261 gctaacgtaa tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg
     4321 tagacgcgcg gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg
     4381 gcaaaagcca cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc
     4441 gccagcgcgc cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc
     4501 aaaataaaga atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc
     4561 agtgtttctg gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag
     4621 ctgaggggaa tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc
     4681 caggcgagat ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat
     4741 tgcataatct gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt
     4801 gtcgcctgac taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg
     4861 gtatgtccgc tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt
     4921 tcatgtggcg gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa
     4981 ccaatcattg ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg
     5041 atcaccgcaa acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt
     5101 accgccagca ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac
     5161 tgcttgcgta gtttgagtac gagagcttcg gctaacagag cactaaccga tgccaacagg
     5221 atctgaacga gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct
     5281 gcgagcaaca ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag
     5341 ctagctattc tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg
     5401 gcctgagcgc gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct
     5461 ggttctgcgt tagtctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct
     5521 tcgacggcgg ctttgcgcgg atcaacctgt tcttctggtt ccgcattagc ctgttgctgt
     5581 tccagtttgc gcgctttggc tcgggcaata gcggcttcga cggcggcttt gcgcggatca
     5641 acctgttctt ctggttccgc attagactgt tgctgttcca gcttgcgcgc tttggcacgg
     5701 gcaatagcgg cttcgacggc ggctttgcgc ggatcgacct gttcttctgg ttccgcatta
     5761 gcctgttgct gttccagctt gcgcgctttg gcgcgggcga tagctgcttc aacggcagtt
     5821 ttacgtggat cagcaacggt tgctgcgtcg ttagtttgct gcagttctgc ctgtttcgct
     5881 ctggcttgcg ctttacgggc ttcccgtgct gcaataattg cactgttatc cgggcgttcg
     5941 cccgctttaa tcacaatagg ctgtgtagcc tgggcctgtt tctctttcac ccgcgccaga
     6001 gcggcagcaa tcgcatcttt atctttggct gcaggttgaa cggctgcgct cttatgtcgt
     6061 tcaaggcgag ccgctttttc gcgctccaga cgagcctggc gcgcttcgaa acgcgctttg
     6121 gcttctgcgg cgcgcttttc ttcctgacga atagccgcaa tttcagcttt ttcctgacgg
     6181 aaatattgca ccaggggaat attgctcggg caaacccacg cgcaagcccc acattcaatg
     6241 caatcagcaa tgttatgcgt ggtagcttta tcgtgttgct gacctttgct gaaccagtac
     6301 aactgttgcg gcaaaagatc cgcagggcag gcgtcagcac aggcgctaca ccggatgcag
     6361 ctttgttctt cctgtggttc gccaagttca ttggcagagg gagccaacag acagttggta
     6421 atctttacga ccgggacatc cagccatggc aaggtaaagc ccattagcgg gccacccata
     6481 atcaccattt gatcggcaga ggggcagaat ccggcatcat tcaataaatg acgcactggc
     6541 gtccccagcc gtgcccagac gttgcccggg cgagcgattg cttcgccagt cagggttaca
     6601 acacgctcgg taatcggctc gccatcaata acggcacgtt tcactgcata agcagtgccg
     6661 acgttttgca ttaatacgcc gatatcggat gaacgcccgc catgtggaac ctgcttcccg
     6721 gtcagaatgt aggttaattg tttagcaccg ccagaaggat atttggttgg aatcacccgc
     6781 agagaaatat cgttagagtc cgccagcacc gcgcgcagca tggaaatcgc ctgcggtttg
     6841 ttatcttcaa tgccgataag aatttcgcgt ggctgcagaa tatgcgcaag aatgcgaata
     6901 ccctctacga cctgagccgc gcaatcctgc atcaaacggt catcggcggt aatgtacggc
     6961 tcgcactcag ccgcgttgat aatcaacgtt tcaatcttat ctccgccacc ctgcaattta
     7021 acgcctgtcg ggaatcctgc accgcccagc ccggctacac caaactgatg tatgcgctcg
     7081 attaactctt cgcgactgcg agtgcgataa tcggcccagc cgtcgcgcgg gatccagcag
     7141 tcttcaccat cggcatcaat aatcacgctt aattcagcta aagctgaagg atgagccgta
     7201 gagtggggcg caatagccgt aacggtaccc gaggtgggcg cgtgaacagg cagcattttg
     7261 ccgcgaccac gggtaagcgg ctggccgcgc aatactttat cgccgacgct aacgcacaac
     7321 tcaccttcag cgccaatatg ctgtttcagt ggaataacaa aacgctgcgc cagtggtacc
     7381 tggcgcaggg gtgtaccgtt ggactgggtt ttcatctccg gtggatggat gccgccgttg
     7441 aaatcccaga ttttattttt tctgaatgca gagaataact taagcatggt gttccacggg
     7501 aatgatacgc acgggaatgg tgttcagatc ccatttccag gagtcaggtg tttctgcgac
     7561 cggttgcaac gagatgcagt gcgtcgggca cggatcaaca cataaattgc agcccgtaca
     7621 gagatcactc attaccgtat gcatggcacg ggtagcgcca acgatggcgt ctaccggaca
     7681 agcctgaata catttagtgc agccaataca gttattttca tcaataaccg ccaccatccg
     7741 cgcaggcgtt agctcttgcg cttcgccatc cagcggctgc ggctcgacat taagcaactc
     7801 ggcaattttt agcatcacag cttcgccacc tggggcgcaa cggttgattt tttcaccgtt
     7861 acagctgatg gcttccgcgt aggggcgaca gccgggataa ccgcactgac cacactggct
     7921 ctgcggtaag atttcgtcaa ttttctcaac gaccggatcg tcttccaccg caaaacggcg
     7981 ggaggcataa cccagaatgg cgccaaacgc caggcccagc aggctcacgg cggcaacggc
     8041 aatccagata gcattcatta caacttcacc aaaccactaa agcccataaa ggccagagac
     8101 ataagacctg cggtaattaa cgcaatggca ttaccgcgaa aaggtgccgg gacatcagcc
     8161 acagcaaggc gttcgcggat ggcggcgaag agcaccatca ccagcgagaa accgacagcg
     8221 gcggaaaaac cgtacagcgc cgactgcaag aaattgtgcc cgagattgat attcagcaac
     8281 gccacgccga gcactgcaca gttggtggtg ataagcggca aaaaaatccc cagcagacgg
     8341 taaagcaccg ggctggtttt gcgcaccacc atctcggtga actgcacgac cacagcaatc
     8401 accagaataa atgccagggt gcgcaggtaa atcagattaa gtgggatcaa aatccacgta
     8461 tcgataagcc aggcgcaaat agacgccagc gtcatcacaa acgttgttgc cagccccatg
     8521 cccatcgcgg tttccagttt tttggaaacc cccataaacg gacagagacc gagaaacttg
     8581 accagtacaa agttattgac cagtacagtt ccgacaaaca gtaacaggta gtcagtcatt
     8641 tttcggcctg aaataaaaaa gccgcctatt atccgttaat ccagagcagg cgacaacagg
     8701 ttaactgtaa ggttattacg gattcacaaa cgtcgctttt acccgcgacg agcgtttgaa
     8761 ataggggacg atcaatgcgg ttgccaacaa agtaaacagc aactggcgaa cggctatgcc
     8821 atcttccacg ggtgaaaaag cgaatgcttt tactgccagc aatacggaaa taagcagcca
     8881 gataatatag tgtttaggaa cacaacgccg acgtttaaag aaagcgatgg tcaaccacag
     8941 tgtgtaatac cacatggcga tagcggtaat aaaagaaacg ccccataaga tttgtgtcgt
     9001 cagtgcttgt ccgccgagcg tttgaaatgt ttgcggagaa gataacgcag cggtatacag
     9061 caacaacgcc agcgtcgtac tcaacagagc gactaaaagc caggccaacg ggccaagcaa
     9121 ccagcctcca atacgttgtg gcgttgtggt ggtcataggt tctccagaat agcgacgcaa
     9181 acaggcaaat ctaattttag gggcgttatt ataaggcttt tataaccgat cgctactttc
     9241 tttattggac atagtgccag acggacttcg gtacctggcc gaggtcaaac aaacgcccgc
     9301 cagaaaccag ctctgcgcga cggtgatcgg cagcacgata catattgatc agttcctgat
     9361 cgtccgtcag ggtgtaatta agatggtcgt agagtttttc cagactttcg agtgaactga
     9421 ttttgcgaaa ttttaataag tagtcctgaa cagtcataaa aggtccataa aataattaat
     9481 accaaagggg taggtattat tagtgatgat aaaatttcac caaaagtgaa cgcttgtgtt
     9541 attacaataa taagaatgcg ctgatggcaa atattcaggg atgaaaaact gctctgaaaa
     9601 gagtaacgcg ctttactatc cctgacaatc accaacaaca tcgaacaaga taataaattc
     9661 ctggtttaat atccgacaag tgaaaacatg cacccggacg ggcagcatgt cgctccacaa
     9721 gtgcagagct tacttgtgtt gtaccgaagc actctgttca ggtggctgat agttgtcaat
     9781 gtgactcgcc acgccaagaa gaatgactga aacgacaaga acgatccaac ctgttaattc
     9841 aataagacga ttcattacag cccacatctc tcttgattga tccattaact tcagggggta
     9901 aatgttactt agcaataata gctccagcaa gatttttact gaggtttttg cgatatttag
     9961 cttttgtcgt tggaaaattc gctatttttt tgacttaagt taaacaacat cccttattgc
    10021 tggcaggtta ttaaactgtt gagcgtgggt aaaggatagt gtcaaatagc catcatactt
    10081 caatgagagg caatgacatg agcgacaaca tccgtgttgg gttgattggg tatggttatg
    10141 cgagcaaaac cttccatgcg cccctgattg cgggcacgcc cgggctggaa ctggcggtaa
    10201 tctccagcag tgatgaaaca aaagtaaaag ccgactggcc aacggttacg gttgtctctg
    10261 agccgaagca tctgtttaac gatcccaaca tagacctgat tgtcattcct acacccaacg
    10321 atacccattt cccgttagcc aaagcggcgc ttgaggcggg taaacatgtg gtcgttgata
    10381 aaccctttac cgtgacactg tcacaagcgc gagagctgga tgcgctggca aaaagcctgg
    10441 ggcgtgtgct gtctgtattc cataaccgtc gctgggatag cgatttcttg acgctaaaag
    10501 gtttactcgc ggaaggcgtg ctgggtgaag ttgcttactt tgagtctcat tttgaccgct
    10561 tccgtccgca ggtgcgcgat cgttggcgtg aacagggcgg cccaggcagc ggtatctggt
    10621 acgatttagc accacatctt cttgatcagg ccattacgct ttttggttta ccggtcagca
    10681 tgacggtaga tttggcacag ttacggcccg gagcgcagtc gaccgattat ttccacgcca
    10741 tcttgtccta tccacagcgg cgagtcattt tacacggtac catgctggca gccgctgagt
    10801 cagcacggta tatcgtgcat ggatcccgag gcagttatgt gaaatatggc ctcgatccac
    10861 aggaagaacg tctgaaaaat ggcgagcgtc taccgcagga agactggggc tacgatatgc
    10921 gtgatggcgt acttacccgc gtggaaggtg aggaacgtgt cgaagaaacg ctgttgacgg
    10981 tgcctgggaa ttatccggct tactatgcgg ctattcgtga tgcgttaaat ggcgatggtg
    11041 aaaatccggt tccggcaagc caggcaatcc aggtaatgga gttgattgag ctgggcatcg
    11101 aatccgccaa acatcgcgcg actttgtgcc ttgcatgaaa aatggatggt gcaatttgca
    11161 ccatccattt tgttacttcg cggcgacttt ttctcgcagt gcgcgttttt cctcagcgct
    11221 gaggaaagcc atttccagac cattaatctg tgcctggcgg atttgctcgc gggataaccc
    11281 agcagctggc gcggcaacgg tatattcgtg aatgatatcc actccctgta cgccgggatc
    11341 gtcagtgtta atgctggcac gaatgccatg ctcaaggaac gttttcagcg gatgtgcagc
    11401 cagctctgct acggtgctgg tctgaatatt ggaggtcaga caggattcaa taccaatttg
    11461 ttgctcggcg agaaaatcca tcagcgcccg atcttcaatg gcttttacgc catgtccaat
    11521 acgctccgca cccagttcac gaatcgcctg ccagatgctt tccggcccgc cagcttcgcc
    11581 tgcatggacg gtaatatgcc agcccgcatc acgcgcgcgg ttgaagtgag aaaggaacag
    11641 acttcccggg aaaccaagtt catcaccggc taaatcaagt gcggtaatct ggtcacggtg
    11701 ggctaaaaag gcctccagct cttgctgaca ggcggcttcg ccgaaggtcc ggctcataat
    11761 gccgataagc ttcgcctgca caccaaaggt gcggcaacct tcacgtacgc catcgatcac
    11821 cgcttcgaca acacccgcta caggcagctg atgtgccatt gccatgtagc ctggtgaaaa
    11881 acgcagctcg acatagtgca ggccgtgacg ggctgcatct tcaatgtttt caaatgccac
    11941 gcggcgacag gcatcaagag aggcgagaac tttaacgccc cagtcaagtt tggtcagaaa
    12001 gctcaccaga tcgggttcgt tggcaatgac ctgaacgtgg ggaatcagtg tttccaggga
    12061 ttgtgcagga agcgagatat tatactggcg gccaagttca agaatggtct ggggacgaat
    12121 gttgccatca aggtggcgat ggatatcagt taatggcagg gtggtatcaa tcatggtcgc
    12181 actctttttc tgaatataaa agtgcgcacc attataaaaa caaagcaggg taaaaatcca
    12241 cgattgcgca acgatttttt acgttgcgca attggggtta gcgaacagcg cggatggcgt
    12301 ttattaatcc agccacacct ttttccagtt tcgaacgtgg gcagccggca ttgagacgga
    12361 caaaaccacg accttcttca ccataggtat accccggcat gatcgcgact ttttcttgct
    12421 cgataagcgc tttttgcaac gcgttgtcgt caatattcaa cggacgtaga tcaagccagg
    12481 ccagataagt ggattgcggg atctgccagt tgagttcagg aaacgcggcg ttcattttat
    12541 ctgagatata cgtcaggtta tctttcagat agactcgtaa ggcatccagc cacggtgcac
    12601 cttgctgata ggcggcgata tgggcagtta acgccagtac cgaaggggaa gaaagcccat
    12661 cacgtccttt cagtgccgat aaataggcat cacggctgct gctattttct ataatcccgt
    12721 aagcaccggt cagcgcggga atattgaaac ttttcgagcc cgacgtcagc aacgcccagt
    12781 ctccccgcgc cacattactc cagggaatat gcggctgctc gccccagacc atatccatat
    12841 ggatttcatc ggaaataacc cgcacaccat gacgctcgca caggtcagcc atgatctcca
    12901 gctcatcgca cgtccacact ttcccggtag gattctgtgg gctacacagg agcataattt
    12961 tacattctgg tttcgccaac acggcttcca gcttgcccat atcgcaaaac cagtcgtcag
    13021 cctgcttctc taaagcaacg ggcattactg tgcgctggtt accttcaatg gccttgtaaa
    13081 atgcgtcata ggcgggcgtg tggatcacca cgccttcacc tgtttcagac cactgacgaa
    13141 tcagttctga aaccatatag atgacagaag ggccatacac caccgtctgc gtatcaattg
    13201 cggtgtaatg ctgggtggaa aaccagtggg caatagccgc gagaaactca tcgtttttcc
    13261 agcggctgta gccaaatacg ccgtgcatca ggcgctgatt cagcgcctcg ataatgcagg
    13321 gggcagtggc aaaatccatg tctgaaatcg tgaacggtaa caggtcagca gtgccgaaac
    13381 ggtcagcgac ataatcccac tgtgtacacc atgtgccatg acgatccacg acctttgaaa
    13441 aatcgaacat atcttatcct tatgcctgga cagtatgcat cagaccggcc atttcatctt
    13501 ttactgactg aacttgtggc ccgataacaa cctgcaggtt atgttgatta agttgtacta
    13561 cgccaattgc ccgattgtcc ttcagtgcct gcacattaac aagcgacata tctttcacag
    13621 acaaacgcag acgggtaatg cagttatcga ggctgacaat attgtcggca ccgcctaatg
    13681 cttcgaggat tgcaggaacg ttgtaacctg atttacccgg cgcaccggca acggcttttt
    13741 cgattgagct ggcaacttcg ctatcgcgcc ccggggtttt cagattgaag cgggtgatag
    13801 cgaaacggaa gatgacgtag taaacgacaa accagattgc cgccaccact ggcaccatgt
    13861 accacttggt tgacagacca tgcaaaatac cgaacaccac gaagtcgatg atattgccgt
    13921 cggtattacc gatggtgacg ccgagcacag acatgacagt gaagccgagg ccggttaaca
    13981 gcgcgtggat gacatacaga actggcgcca cgaacaggaa caggaattcc agcggttcgg
    14041 tcgtgccgcc aacgacacag gcgatcaggc cagaaatcag cagaccttta attttatggc
    14101 gattttccgg gcgcgcgcag tgatacatag ctaacgctgc acctggcaga ccgccgagaa
    14161 acgcaggcat tttaccttgc gaaaggaaac gcgtggcgct ttcagaaaaa ccgtgagtgg
    14221 tcgggcaact caattgcgcc tggaagatgg tcagtgcgcc gctgacggtt tgaccgcaga
    14281 cttcctgcgt gccgcctgcg tcggtaaagc gaattaatgc caccagaatg tgatgcagac
    14341 caaacggcaa cagcagacgt tcaccggtac caaacagcat cggtccgaaa tcacccgcgc
    14401 tgtttatcat atggcccaag ccgctaatac ccatggcgaa aatcggccag actaatggaa
    14461 tcaccaggcc gataaggccc atcaccagcg aggagataat tggtacgaag cgcgtaccgc
    14521 cgaagaatgc cagcgcatcc ggcaggcgga tattatggaa acgctcatgc agcatccaga
    14581 cgataatacc ggcgatcacc gcaccgagga tcccggtatc gatcgactgg atcccaagaa
    14641 tgctctggat gttattggct ttcagaaccg cggcatccgt ggttggcata atgcctttat
    14701 tggtcaacca gaagtttacc gcgaggttca ttaccgcata accgacgaag ccagcgaatg
    14761 ccgctacgcc tttattttcg cgtgccaggc ccagcgggat ggcgatacag aacatgacag
    14821 gcaggaaact aaaagcaaac gagccaatct tactcatcca ggtaaagata gcctgcaaca
    14881 cggggttgcc caggaccggg atcagggtta tgacatcatg gctgctaaga gaactaccaa
    14941 tgccgagcat aatgccgcag aacgacaata atgccacggg taacatgaag gttttgccta
    15001 actgctggaa gaactcccac agcgtgactt tcggtgctgt tttcgccgtc ataaaacgac
    15061 tcctcgtaga gaacaatctg gattcagtaa attgcgcgag aagataaaac gttttatcaa
    15121 attttagtga ggcataaatc acattacgca acgataatag cgggtataag ataaataaaa
    15181 ggtaaaacgt tttatctgtc acataatcag ggagtaggtc atctgcatgg ctaccgccaa
    15241 aaaaataacc attcatgatg ttgcgctggc tgcgggcgtg tcggtaagta ccgtttcgct
    15301 ggtgcttagt ggcaaagggc gaatctctac cgccacagga gaacgcgtta acgccgccat
    15361 tgaagagctg ggatttgtgc gcaatcgcca ggcgtcggcg ctgcgcggcg ggcaaagcgg
    15421 cgtcattggt ttgatcgtcc gtgatttatc tgcgccgttt tacgccgaat tgacggccgg
    15481 attgacggaa gctctggaag cgcagggacg gatggttttt ttgcttcacg gcggtaaaga
    15541 cggcgagcag ctggcacagc ggttttcact gttactgaat cagggtgtgg atggtgtggt
    15601 aattgccggg gctgcaggaa gcagcgatga cctgcgacgg atggcagaag aaaaagctat
    15661 cccggtgatt ttcgcttccc gtgccagtta tcttgatgat gttgatacgg ttcgcccgga
    15721 caacatgcag gctgcacagt tgttgacgga gcatctcatt cgcaatgggc atcagcggat
    15781 cgcctggctg ggagggcaaa gttcctcatt aacccgtgca gaacgggtgg ggggctattg
    15841 tgcaactcta ctaaaatttg gcctgccgtt tcacagcgat tgggtgctgg agtgcacatc
    15901 cagccagaag caagccgcgg aagctatcac ggcgctttta cgtcataacc cgaccatcag
    15961 tgctgtggtt tgctataacg aaactattgc gatgggggca tggtttggtt tgctcaaagc
    16021 agggcggcaa agcggggaaa gcggagtcga tcgttacttt gagcaacaag tttcgctggc
    16081 ggcatttacc gatgcgacac caaccacact tgatgatata cccgttacct gggccagcac
    16141 gcctgcgcgg gaacttggta ccacacttgc ggatcgcatg atgcaaaaaa tcacccatga
    16201 agagacgcat tcacgcaatc ttattattcc cgcccggctc attgcggcga aataatcctc
    16261 tctttatctg ctatacctgg tagtgtcctt tcctcaaggt taatgataaa aaatgtggca
    16321 cacaggcggt gccttttatt tttactgaag ttcacaggag gtttatgtgt ttaattctga
    16381 caacctgaga ctcgacggaa aatgcgccat catcacaggt gcgggtgcag gtattggtaa
    16441 agaaatcgcc attacattcg cgacagctgg cgcatctgtg gtggtcagtg atattaacgc
    16501 cgacgcagct aaccatgttg tagacgaaat tcaacaactg ggtggtcagg catttgcctg
    16561 ccgttgtgat attacttccg aacaggaact ctctgcactg gcagactttg ccgtcagtaa
    16621 gctgggtaaa gttgatatcc tggttaacaa cgccggtggc ggtggtccta aaccgtttga
    16681 tatgccaatg gcagattttc gccgtgctta tgaactgaat gtgttttctt ttttccatct
    16741 gtcacaactt gttgcgccag aaatggaaaa aaatggcggt ggcgttattc tgaccatcac
    16801 ttctatggcg gcagaaaata aaaatataaa catgacttcc tatgcatcat ctaaagctgc
    16861 ggccagtcat ctggtcagaa atatggcatt tgacctgggt gaaaaaaata ttcgggtgaa
    16921 tggcattgcg ccgggggcaa tattaaccga tgccctgaaa tccgttatta caccagaaat
    16981 tgaacaaaaa atgttacagc acacgccgat cagacgtctg ggccaaccgc aagatattgc
    17041 taacgcggcg ctgttccttt gctcgcctgc agccagctgg gtaagcggac aaattctcac
    17101 cgtctccggt ggtggggtac aggagctcaa ttaatacact aacgaaccgg taaacagccg
    17161 tatgtgttgt ttaccgggat agcgtgtgca attcatcaac gtctctgcta aacaactggc
    17221 agccaaatca cggctattgg ttaaccaata gatggatgaa aagcatacga atagagtgtg
    17281 ccttcgcact attcaacagc aatgataggc gctcacctga caacgcggta aactagttat
    17341 tcacgctaac tataatggtt taatgatgga taacatgcag actgaagcac aaccgacacg
    17401 gacccggatc ctcaatgctg ccagagagat tttttcagaa aatggatttc acagtgcctc
    17461 gatgaaagcc atctgtaaat cctgcgccat tagtcccggg acgctctatc accatttcat
    17521 ctccaaagaa gccttgattc aggcgattat cttacaggac caggagaggg cgctggcccg
    17581 tttccgggaa ccgattgaag ggattcattt tgttgactat atggtcgagt ctattgtctc
    17641 tctcacccat gaagcctttg ggcaacgggc gctggtggtc gaaattatgg cggaagggat
    17701 gcgtaaccca caggtcgccg ccatgcttaa aaataagcat atgacgatca ctgaatttgt
    17761 tgcccagcgg atgcgtgatg cccagcaaaa aggcgagata agcccagaca tcaacacgtc
    17821 aatgacttca cgtttactgc tggatctgac ctacggtgta ctggccgata tcgaagcgga
    17881 agatctggcg cgtgaagcgt cgtttgctca gggattacgc gcgatgattg gcggtatctt
    17941 aaccgcatcc tgatcctctc atttcgcggg ctggtgataa ctgtgcccgc gtttcatact
    18001 ttaatttccc tgtacaaaat acaatctagc cgggatcgga gaattccccc ccaaaaatat
    18061 tcaccgtagc catatgtcat gagagtttat cgttcccaat atactcgaac gaacgttcgg
    18121 ttgcttattt tatggcttct gtcaacgccg ttttaaagat taatgcgatc aatatcacgc
    18181 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
    18241 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
    18301 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
    18361 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
    18421 actgtggaat tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg
    18481 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg
    18541 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
    18601 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
    18661 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
    18721 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
    18781 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
    18841 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
    18901 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
    18961 tggccaatgg tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
    19021 gacaaggcac tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
    19081 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
    19141 ttcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
    19201 cgttctactt tactggcttt ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg
    19261 ataacgtgct gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc
    19321 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
    19381 tggtgattga tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
    19441 cgggcaacaa gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag
    19501 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
    19561 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
    19621 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
    19681 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
    19741 gttattacgg atggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
    19801 aagaacttct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
    19861 tggatacgtt agccgggctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
    19921 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
    19981 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
    20041 agaaagggat cttcactcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
    20101 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgaatcaaca
    20161 actctcctgg cgcaccatcg tcggctacag cctcggtgac gtcgccaata acttcgcctt
    20221 cgcaatgggg gcgctcttcc tattgagtta ctacaccgac gtcgctggcg tcggtgccgc
    20281 tgcggcgggc accatgctgt tactggtgcg ggtattcgat gccttcgccg acgtctttgc
    20341 cggacgagtg gtggacagtg tgaatacccg ctggggaaaa ttccgcccgt ttttactctt
    20401 cggtactgcg ccgttaatga tcttcagcgt gctggtattc tgggtgccga ccgactggag
    20461 ccatggtagc aaagtggtgt atgcatattt gacctacatg ggcctcgggc tttgctacag
    20521 cctggtgaat attccttatg gttcacttgc taccgcgatg acccaacaac cacaatcccg
    20581 cgcccgtctg ggcgcggctc gcgggattgc cgcttcatta acctttgtct gcctggcatt
    20641 tctgatagga ccgagcatta agaactccag cccggaagag atggtgtcgg tataccattt
    20701 ctggacgatt gtgctggcga ttgccggaat ggtgctttac ttcatctgct tcaaatcgac
    20761 gcgtgagaat gtggtacgta tcgtggcgca gccgtcattg aagatcagtc tgcaaaccct
    20821 gaaacggaat cgcccgctgt ttatgttgtg catcggtgcg ctgtgtgtgc tgatttcgac
    20881 cttcgcggtt agcgcctcgt cgttgttcta cgtgcgctat gtgttaaatg ataccgggct
    20941 gtttactgtg ctggtactgg tgcaaaacct ggtcggtact gtggcatcgg caccgctggt
    21001 gccggggatg gtcgcgagga tcggtaaaaa gaataccttc ctgattggcg ctttgctggg
    21061 aacctgcggt tatctgctat tcttctgggt ttccgtctgg tcactgccgg tggcgttggt
    21121 tgcgttggcc atcgcttcaa ttggtcaggg cgttactatg actgtgatgt gggcactgga
    21181 agctgatacc gtagaatacg gtgaatacct gaccggcgtg cgaattgaag ggctcaccta
    21241 ttcactattc tcatttaccc gtaaatgcgg tcaggcaatc ggaggttcaa ttcctgcctt
    21301 tattttggga ttaagcggat atatcgccaa tcaggtgcaa acgccggaag ttattatggg
    21361 catccgcaca tcaattgcct tagtaccttg cggatttatg ctactggcat tcgttattat
    21421 ctggttttat ccgctcacgg ataaaaaatt caaagaaatc gtggttgaaa ttgataatcg
    21481 taaaaaagtg cagcagcaat taatcagcga tatcactaat taatattcaa taaaaataat
    21541 cagaacatca aaggtgcaac tatgagaaaa atagtggcca tggccgttat ttgcctgacg
    21601 gctgcctctg gccttacctc tgcttatgcg gcgcaactgg ctgacgatga agcgggacta
    21661 cgcatcagac tgaaaaacga attgcgcagg gctgataagc ccagtgctgg cgcgggaaga
    21721 gatatttacg catgggtaca gggaggattg ctcgatttca atagtggtta ttattccaat
    21781 attattggcg ttgaaggcgg ggcgtattat gtttataaat taggtgctcg tgctgatatg
    21841 agtacccggt ggtatcttga tggtgataaa agttttggct ttgccctagg ggcagtaaaa
    21901 ataaaaccca gtgaaaatag cctgcttaaa ttaggtcgct tcgggacgga ttatagttac
    21961 ggtagcttac cttatcgtat tccgttaatg gctggcagtt cgcaacgtac attaccgaca
    22021 gtttctgaag gagcattagg ttattgggcg ttaacgccaa atattgatct gtggggaatg
    22081 tggcgttcac gagtattttt atggaccgat tcaacaaccg gtattcgtga tgaaggggtg
    22141 tataacagcc agacgggaaa atacgataaa catcgcgcac gttctttttt agccgccagt
    22201 tggcatgatg ataccagtcg ctattctctg gggggatcgg tacagaaaga tgtttccaat
    22261 cagatacaaa gtattctcga gaaaggcata ccgctcgacc cgaattatac gttgaaaggg
    22321 gagttgctcg gcttttacgc gcaactcgaa ggtttaagtc gtaataccag ccagcccaat
    22381 gatacggcgt tggttagtgg acaagtgacc tggaatgcgc cgtggggaag cgtatttggc
    22441 agtggtggtt atttgcgcca tgcaatgaat ggtgccgtgg tggataccga cattggctat
    22501 cccttttcat taagtcttga tcgtaaccgt gaaggaatgc agtcctggca attgggcgtc
    22561 aactatcgtt taacgccgca atttacactg acatttgcac cgattgtgac tcgcggctat
    22621 gaatccagta aacgagatgt gcggattgaa ggcgcgggta tcttaggtgg tatgaactat
    22681 cgggtcagcg aagggccgtt acaagggatg aatttctttc ttgctgccga taaagggcgg
    22741 gaaaagcgcg atggcagtgc gctgggcgat cgcctgaatt actgggatgt gaaaatgagt
    22801 attcagtatg actttatgct gaagtaagaa ataacgccgg agagaaaaac ctccggcgtt
    22861 tcagattgtt gacaaagtgc gcgtttttta tgccggatgc ggcgtaaacg ccttatccag
    22921 cctacaaaaa ctcataaatt caaagtgttg caggtaaagg taggcctgat aagcgtagcg
    22981 catcaggcaa tctctggttt gttttcagat gaaaacgccg gagtgaaaat tctccggcgt
    23041 tttggtcgtg aattactgct gcggaattgc cggtacagcc ggaacgttaa gagctggcat
    23101 cgcaaacatg ccaacaaaat cttctaacga cattttctgc ccatttaacg ttatctgacc
    23161 gttagcatat tgcaggctgg tggtgatggt attgtcctgc aaggtggtca gacggaacat
    23221 ctgccccatt gctgatgcac cttcaacttg ctgtttcgcc agttttttcg cttgatcttc
    23281 ctgataacct tccagcttcg ctacctgagt cataaactca gttgccatat ccaccggaat
    23341 ggtcagtttc gcatccagag atttaaccga gcggtccact tcctgcgcca gcgtttgcgg
    23401 cgcttcttta gtcgttgccg gatctttcag gaacagcgac agattcagcg cactttcacc
    23461 ctgactgttt ttccagctta gcggtgcgat agtaatcacc ggatcgcctt tcaacatcag
    23521 cggcagggcg ctaaagaagg cttccgtcac tttctcctga taaagtgcgg gattgttggc
    23581 aatttctggc tgtgccagca gcgcctgagt ttgcgcgtta tattgctggc taaactgatg
    23641 ccaggcttca ccatcaatct ggccgacttt taaagtcagc ttgccgctgc ccagatcctg
    23701 attctgtacc ttcaggctgt ttagcgagta atccag
//
LOCUS       DAFATA010000066        23563 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFATA010000066 DAFATA010000000
VERSION     DAFATA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.067"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..483)
                     /locus_tag="LGJ20_003103"
     CDS             complement(<1..483)
                     /locus_tag="LGJ20_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK2387130.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     A"
     gene            complement(496..1026)
                     /gene="rppH"
                     /locus_tag="LGJ20_003104"
     CDS             complement(496..1026)
                     /gene="rppH"
                     /locus_tag="LGJ20_003104"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBK2387131.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            1711..2400
                     /gene="mutH"
                     /locus_tag="LGJ20_003105"
     CDS             1711..2400
                     /gene="mutH"
                     /locus_tag="LGJ20_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBK2387132.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2469..3182
                     /locus_tag="LGJ20_003106"
     CDS             2469..3182
                     /locus_tag="LGJ20_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBK2387133.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            3320..3538
                     /gene="ygdR"
                     /locus_tag="LGJ20_003107"
     CDS             3320..3538
                     /gene="ygdR"
                     /locus_tag="LGJ20_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBK2387134.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            3646..4686
                     /locus_tag="LGJ20_003108"
     CDS             3646..4686
                     /locus_tag="LGJ20_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBK2387135.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(4718..5911)
                     /gene="lplT"
                     /locus_tag="LGJ20_003109"
     CDS             complement(4718..5911)
                     /gene="lplT"
                     /locus_tag="LGJ20_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBK2387136.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(5904..8063)
                     /gene="aas"
                     /locus_tag="LGJ20_003110"
     CDS             complement(5904..8063)
                     /gene="aas"
                     /locus_tag="LGJ20_003110"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBK2387137.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            8649..9680
                     /gene="galR"
                     /locus_tag="LGJ20_003111"
     CDS             8649..9680
                     /gene="galR"
                     /locus_tag="LGJ20_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBK2387138.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(9687..10949)
                     /gene="lysA"
                     /locus_tag="LGJ20_003112"
     CDS             complement(9687..10949)
                     /gene="lysA"
                     /locus_tag="LGJ20_003112"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK2387139.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            11071..12006
                     /gene="lysR"
                     /locus_tag="LGJ20_003113"
     CDS             11071..12006
                     /gene="lysR"
                     /locus_tag="LGJ20_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBK2387140.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(11993..12685)
                     /gene="ygeA"
                     /locus_tag="LGJ20_003114"
     CDS             complement(11993..12685)
                     /gene="ygeA"
                     /locus_tag="LGJ20_003114"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBK2387141.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(12814..14232)
                     /gene="araE"
                     /locus_tag="LGJ20_003115"
     CDS             complement(12814..14232)
                     /gene="araE"
                     /locus_tag="LGJ20_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBK2387142.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(14540..15301)
                     /gene="kduD"
                     /locus_tag="LGJ20_003116"
     CDS             complement(14540..15301)
                     /gene="kduD"
                     /locus_tag="LGJ20_003116"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBK2387143.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(15331..16167)
                     /gene="kduI"
                     /locus_tag="LGJ20_003117"
     CDS             complement(15331..16167)
                     /gene="kduI"
                     /locus_tag="LGJ20_003117"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBK2387144.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(16454..17635)
                     /locus_tag="LGJ20_003118"
     CDS             complement(16454..17635)
                     /locus_tag="LGJ20_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBK2387145.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            17890..19119
                     /locus_tag="LGJ20_003119"
     CDS             17890..19119
                     /locus_tag="LGJ20_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBK2387146.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            19579..20211
                     /locus_tag="LGJ20_003120"
     CDS             19579..20211
                     /locus_tag="LGJ20_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2387147.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMAGKINELFIRSHSVQH"
     gene            20544..21353
                     /locus_tag="LGJ20_003121"
     CDS             20544..21353
                     /locus_tag="LGJ20_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2387148.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
     gene            21346..21828
                     /locus_tag="LGJ20_003122"
     CDS             21346..21828
                     /locus_tag="LGJ20_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387149.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMFNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLVQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(21861..21941)
                     /locus_tag="LGJ20_003123"
     CDS             complement(21861..21941)
                     /locus_tag="LGJ20_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387150.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(21977..22401)
                     /locus_tag="LGJ20_003124"
                     /pseudo
     CDS             complement(21977..22401)
                     /locus_tag="LGJ20_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22595..23085
                     /locus_tag="LGJ20_003125"
                     /pseudo
     CDS             22595..23085
                     /locus_tag="LGJ20_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23310..>23563
                     /locus_tag="LGJ20_003126"
     CDS             23310..>23563
                     /locus_tag="LGJ20_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK2387151.1"
                     /translation="MSTETIEIFNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVC"
BASE COUNT         6490 a         5798 c         5282 g         5993 t
ORIGIN      
        1 ggcagtcaac tgcgactgag aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa
       61 ggattcttca ctttcgtcat actggcgcaa ctctcgttgc tgtaccacca gtacaccaag
      121 caactggcga cgttgaataa ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac
      181 ggaggggatg tatttgaagc tggggtgctt ttgcgcatct gcaaggttta tcggttccgc
      241 cagcctgcca accaggccga cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg
      301 tggttttttc agcccccggg tcgccatcag gtagtaacaa cgtcgatcat gatcggccag
      361 gtagaccgaa cagacctcgg tatccatcgc aagacagatg tcggtaacca gaatatttaa
      421 cgcctcattc aggcgtggtg cgctggctac cttttcgact atttcgcgca ggcgagtgag
      481 cataattggc gtgacttaac ctcttttacg tcgataagca gatgcgtttt gtggttttgg
      541 cgtattttcc tgcagtgaca tcaccacact cgcgaactct ttcattaccc tacggtagac
      601 atcacgttta aatgacacca cctgtctgac cggataccag taacttaccc atcgccagcc
      661 gtcaaactct ggtgtactgc tggtttgcat attgatttct gcatcgccgc tcaccagctg
      721 caagagaaac catttttgtt tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa
      781 acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa gcaaggattc gaacgtcttt
      841 gcggcttaat cctacttctt caaacaattc acggtacatc gcctgctctg cggattctcc
      901 ggggttgatt ccgccttgcg gaaattgcca ggagtgctga ccaaatcgcc gggcccacat
      961 tacctgcccc tggcgattac aaatcacgat acctacgttt gggcggtagc catcgtcatc
     1021 aatcaccgga ctacctcaaa ataaagcttt atatacgaat gattgtttca tactccagga
     1081 agacggtaaa ccactctctg cagggcatta cacactaata acaattgaat aactcacagt
     1141 tatgtgcaga gttataaaca gaggaagggg tggatagccg tttttattca ccttttctgt
     1201 ggatagagtt gtgaagaact acggaattac tacgggaaaa cccggagcat tctgaataag
     1261 cccgatatag acattttaaa atattcatta ttagtcaatg cattacatgt aatttgagta
     1321 atgaaaaaga gtaattcgtg acccaggtca cacctctcat ttacgggttg gcgaaagatc
     1381 aaccaatgcc gtatttatcc acagaatgtg ccactaagtt aagcactgaa ccactaaaaa
     1441 ctggagtttc atcgcacgtc aaggctgtaa atggaaacag tagtggaggt ttttcacagt
     1501 tatcccagct ttctgtggat aacatggtgt aagatcctgt ttattttcag tgaccagatt
     1561 tggaaaacct attgcagtgt tgcgcaactc gattaccggc agcctaaaaa gcaatataaa
     1621 tcagggaatt gaacaacaca tgtggaaaaa gttacactgc gaatattcgg cacataattg
     1681 ctgtttgttt tttaatcaag gtatcatgac atgtcccaac ctcgcccact gctctctcct
     1741 cccgaaactg aagaacagtt gttagcgcaa gcacagcaac tttctggtta tacattggga
     1801 gaactggcgg cacttgccgg gcttgttacg ccagagaatt taaaacgcga taagggctgg
     1861 attggcgtgt tactggagat ctggctaggt gccagcgcag ggagtaaacc tgagcaagat
     1921 tttgccgctc tgggcgtgga acttaaaact atccctgtgg atagtcttgg tcgtccgctg
     1981 gaaacaacat tcgtttgtgt tgccccgtta acgggtaata gcggggtgac ctgggaaacc
     2041 agccatgtgc gccacaagct caaacgcgta ctgtggatac cggttgaagg cgagcgcagc
     2101 atcccgctgg cgcagcgtcg cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa
     2161 gaccgacagc tacgcgaaga ctgggaagaa ttaatggata tgattgttct cggtcaggtt
     2221 gagcggatca ccgctcgtca cggggaatat ttacagataa gaccgaaagc agcgaatgcg
     2281 aaagcgctta ccgaagccat tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc
     2341 ttttatttga agaagaattt caccagtgca ctactggccc gtcattttct gatccagtag
     2401 ccatcgcttt gacctgccgc tttccgggca tataattacc gcttcatttt tttggcaggg
     2461 ctttttagat gttatttgca tggataaccg atcctaacgc ctggcttgcg ctcggtacgc
     2521 tgacgctgct ggagatcgtt cttgggatcg acaatattat tttcctttct ctggtggtgg
     2581 caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg
     2641 ttatgcgtct ggcgctgctg gcatcaatcg cctgggttac gcgcctgaca aatccgcttt
     2701 ttacaatatt cagtcaggaa atttctgccc gtgatttgat tctgcttctg ggtggcttgt
     2761 tccttatctg gaaagccagc aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc
     2821 tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca gattatgctg ctggatatta
     2881 tctttagcct cgactcggtg attacggctg tgggtctgtc agatcacctg tttattatga
     2941 tggcagccgt ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt
     3001 ttgtcgaacg ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct
     3061 ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa aggttacatc tacttcgcga
     3121 tgttcttctc tattgcggtt gaaagcctca acttgattcg caacaaaaag aatccgctct
     3181 gatactccgt acgctctcct gcgggagggc gtactcactc cttttaccct cacagtttaa
     3241 gattttcctg ctttcaagat atataacgtc ggtttataaa cagactatta tcataggtag
     3301 gccaaatgag gataaccgga tgaaaaaatg ggcagtaata atttctgcag tcggactggc
     3361 gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg accaaagatg gccgtatgat
     3421 tttgaccgat ggaaaacctg aaattgatga tgataccggg ctggtgagtt atcacgatca
     3481 gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca
     3541 aataaggtca gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg
     3601 ccatttttat attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt
     3661 ataccccaca gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa
     3721 cagaacagcg aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac
     3781 cttatcgacg ttgccgaaat gtacccagta cctccgcgcc ccgaaacgca agggttaacc
     3841 gaaacctacg tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc
     3901 tccaaagtga gcggaccgtc gcgcaataat gacaagggca tccgcccgga tcaggcgctg
     3961 gatcggaaga atatccgcga agcgctgcat gacagcctca agcgcctaca gactgattac
     4021 ctcgatcttt atcaggtgca ctggccgcag cgcccgacca actgcttcgg caaactcggt
     4081 tatagctgga cggattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca
     4141 gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac tgcatttggc
     4201 gtaatgcgct acctgcatct agcggacaaa cacgatctgc cgcgtattgt caccattcag
     4261 aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat
     4321 gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat
     4381 ctcaatggtg caaaacccgc tggcgcacgt aatacgctct ttagtcggtt cacccgctat
     4441 agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatggc
     4501 ctggaccctg ctcagatggc gctcgcgttt gtacgccgtc aaccgtttgt tgccagcact
     4561 ctgctgggcg caaccacgat ggatcagctg aaaactaaca tcgaaagttt gcatctggag
     4621 ttaagcgaag acgtattagc tgaaattgaa gcggtgcatc aggtttatac ttatccggca
     4681 ccataagatt aacgccggtt aaaaccggcg ttaaatatta atgacggcgc tgccagatcc
     4741 acagcgccgt tattgccagc gcaaacagcg caccgaagcc aatgccaatg ggcacgaccg
     4801 ggatgcctat cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt
     4861 cgccaaggtt ttgtactgca atcgcattcc ccgccccgac gcttttttta ccccgctcct
     4921 gtagcaacgc attgagcgga acgacaaaaa agccccccat cacgccaatc agcatcaaca
     4981 aggcataggc tggcagcagc tcgtgttgca gggaaaaaat cagtaccacc acgccaatca
     5041 aaatcccggc tggcatacag cgtgacacgg tttccagcgt aactaacttc gctgccgcac
     5101 ctgcgccaac cacgatacca atcgctacca tcgcgttgag ataggtgggc gtagcgttat
     5161 cggtaatgcc cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacccg
     5221 ctccccagaa taaactggtg cccaccagcg aaaaacgcgt ttcaccattg cgccatagcg
     5281 aggtgcaggc attcaggaaa ctgcgggtca tgttgatgag attccaggac tgccccggac
     5341 gcgccgccgc cagtttggga atgtagatat tggcaacgac cgcaccaccg taggccagtg
     5401 cgcatgcggc cagggcgacg aggacatgcc agtcagccag cacaccaccg gctacggaac
     5461 cgagcaaaat cgccgctatg gtagaagctt ccattaaacc gttagctttc actaacttac
     5521 tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa
     5581 cacccaccag cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca
     5641 gcagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct
     5701 gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa caccatttgc aggatgggct
     5761 ggctccactc cggatagaac tgcgctttca gtaacgccag agtggcaaac agtagggcat
     5821 tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca
     5881 acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt
     5941 ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga
     6001 atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgcaactta
     6061 tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg
     6121 ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag ttgttccacc
     6181 atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc
     6241 tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag tgtcatacca gccgcgctcc
     6301 atttcgccgc gaacattctc ggcggtgggc acttccagta cacctggctt ctccacccgc
     6361 agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacca
     6421 gggaccgaca acaggcgcgc atccattcct ggtagaatac gccctaccgt accgggtttc
     6481 gccgccatcg gtacgttgat agaaacgaca ggcgcgcact cggttacacc atagccttca
     6541 aggatgcgca ggccaaattt atcctgccaa agctgtttgg tactttcttg tagtttttct
     6601 gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcgaa acgcgcgtag
     6661 tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata caccaactcc
     6721 ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtaagcagt
     6781 ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaatgc cgacataaag
     6841 cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt
     6901 ttatggctat ggacgacgcc ttttggatgg ccttcagaac cggaggtaaa gaggatcagc
     6961 gcctcttctt ccggctgctg ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag
     7021 atccatactt tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc
     7081 acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctggcgggaa
     7141 gtgaagatgg ttttgatttc agccgccgta atagcactgg tcagcccttt taccccagca
     7201 gtgtagttca tcattgcggg aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca
     7261 ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact gtatttttca
     7321 aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc tggggtaaag
     7381 ttgacgtctt cgacacattt cttcccggct ccgaagcggt acattgcact cagtaaagat
     7441 tcgtacagag tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc
     7501 atttcaccag cgattttgcg acggtcacgg gcacgcggcg catccggcat cgccacctgc
     7561 gttggtggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac cagacctttc
     7621 aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca
     7681 gacttcgccg cgacaaaacc cgcgccatcg tagattttca tcagcgagcc tgtagtggtg
     7741 atgcgtcctt cagggaaaat caccactggt cggccctgtt ccaccagacg taccagatgt
     7801 ttaatagcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa
     7861 cgcatatacc actgttggct tattgaggtg taaacggcaa acactggacg cacaggtaaa
     7921 aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat taggcgtaat tagaacgcgc
     7981 tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa
     8041 tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg
     8101 gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc
     8161 tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg aataatctca
     8221 ccaatcaata cctctgggat cttgattgtg gtctgcacga cgactcttcg ccagcgagaa
     8281 aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga
     8341 ttgatgaccg gccaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca
     8401 acccgaagtt gacttcacct atcaatacct ctgggatcac cactttagca acctgaagcc
     8461 aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat
     8521 ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacgatgaa aacacgccac
     8581 cccttgaacc aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt acccactaag
     8641 gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg
     8701 tttcccgcgt cattaataat tcacctaaag ccagcgaagc ttcccggctg gctgtgcata
     8761 gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga
     8821 ccactgaaac ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg
     8881 tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt
     8941 accacaacga acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg
     9001 cgttggtcgt ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa
     9061 tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc
     9121 ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc
     9181 gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt
     9241 attacgatgc ccttgctgaa agtggtattg cggccaatga ccggctggtg acatttggcg
     9301 aaccagacga aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggaagaaatt
     9361 tcactgcggt agcctgttat aacgattcaa tggcggcggg tgcgatgggc gttctcaatg
     9421 ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt
     9481 cacgctatgt gcgcccgcgc ctgaccaccg tgcgttaccc aatcgtgacg atggcgaccc
     9541 aggctgccga actggctttg gcgctggcgg ataatcgccc tctcccggaa attactaatg
     9601 tctttagtcc gacgctggta cgtcgccatt cagtgtcaac tccgtcgctg gaggcaagtc
     9661 atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg
     9721 gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc
     9781 ggacggctgt tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc
     9841 agataatcac ccgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc
     9901 tgggtaaaga catcgcccga ttcacataat ggcccggcga cgacggtttc caccgttggc
     9961 gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac
    10021 attgccgggc gcatcagatc gttgaaccca gcatcaacca gcacaaagtg gcggctaccc
    10081 atttgtttga cgctccgcac ctgagtaatt aatacgccag actgcgctac caggaagcga
    10141 cccggttcaa tttccagttt cacagggtgg cccaaatggc gggcgatttg ctcacgcgcg
    10201 gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga
    10261 acagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gatgacctga
    10321 cgcaccatag caccacacac ctgttccaga tgggcataat caacgccaga accaatgtgc
    10381 atgtgaatgc cgaccagctg cagatgatga cgttgtatca cgtccagtgc ggcgggcaga
    10441 tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt
    10501 ccgtgaccaa accccgggtt aacgcgcagc catacccgat gccctggcga aacctggccc
    10561 agttggtcga gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt
    10621 tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc
    10681 ggattgtaac ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc
    10741 acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag
    10801 cgcaccacat caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag
    10861 acccacaccg ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg
    10921 gtatcggtgc tgaacagtga atgtggcata acaaactcca gatgagtgct tttttatgat
    10981 tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgagtctat gcaaaaatga
    11041 tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa
    11101 atttttcatg cggtaatgac cgccggaagc ctgactgagg cagcacacct gctacacacc
    11161 tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctaaaattg
    11221 tttgagcgcg tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa
    11281 gtgcaacgat cctggtacgg actggatcgc attgtcagcg ccgcagaaag tctgcgcgag
    11341 tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg
    11401 cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag
    11461 gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag
    11521 acactccata cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt
    11581 gtgttacctc cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag
    11641 ggtgagaact acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta
    11701 tttactgaac atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc
    11761 tgcgcaatgg tacgggcggg ggtaggtgtt tcggtggtta acccgctcac cgcactggat
    11821 tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc
    11881 ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag tgagcattta
    11941 caagcggggt taccgaaact ggtcacttct cttgacgcga ttttgtcgtc agctacgaca
    12001 gcataaaagc gacagcatcc tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac
    12061 tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc
    12121 cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt
    12181 gccccagaca cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag
    12241 gaataagaca attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca
    12301 tggtgtaacg cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc
    12361 cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg tgactcaatg gcatccgcca
    12421 ctttatgcat cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa
    12481 gcgccgcctc agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt
    12541 ctatttcatg aaaatcgacg ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc
    12601 gctgtttaat gccttcattt atcagacgat agtaaggaat ggtggattcc cagctcattc
    12661 ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct
    12721 gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc gatctctatt aacgaaaaaa
    12781 gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttctc
    12841 gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag
    12901 ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc
    12961 agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg ataatcatat tcgacaccca
    13021 gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga
    13081 gcacaggatc cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt
    13141 catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag
    13201 gcaatagccc agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg
    13261 tttacgacct gctttatcta ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag
    13321 tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa
    13381 gatacgcggc gcatagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa
    13441 caacataccg agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc
    13501 gccttgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga
    13561 cgtatcgcgc agcatacgca acacttcttc cgcctcaata tgacgcccct tttccgccag
    13621 ccagcgcggg ctatttggca ggaagaccac cagaataatc agcaggactg ctggtaaagc
    13681 aagaaccccc aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa
    13741 cgccagcacg atgccgagtg tgaccatcaa ctggtacata ctgatcatct taccgcgaac
    13801 gttttcactt gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc
    13861 aatgcccagc accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc
    13921 tatagaaccg agtacaaaca ggatggcccc cgccatcagg ctgtatttac gacccaggcg
    13981 gaacgacagc caaccattaa acagcgcacc aattgctgcg ccgagcatca tgctactaac
    14041 cacccattcc tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc
    14101 ggcgattacg ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac
    14161 aaacatattc atacgccgcg tatcccgcaa agaacgtggc gttaaagcag attccgtatt
    14221 gatagtaacc attttttcct gccagcagag agtaagacat agtgaaaaaa tacgtgaaca
    14281 actcacgcag gtgtcaggtc ggaaacagca taaatatgga ttaaattgct acgacatgtc
    14341 gttatgtgat ggatattcca attttcaaat taagttgaat tattgatatt attattaacc
    14401 acctaatttt acagcagata aaattcataa agttcattaa ttgatatgga ttatttcata
    14461 accatgatat ggattatgat gatctacaga tataaaaaac cctgccatgc ggcagggtca
    14521 taaaagtaag aagaatgaat taacgcgcca gccaaccgcc atccacggca atggtataac
    14581 cattcacata atctgaagcg ctggaggcaa ggaacactac cggccccatc aggtcactcg
    14641 gcagtcccca acggccagct ggaatgcggt cgaggatttc cgcgctacgt tgttcatctg
    14701 cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg ggctatcgca ttaacattaa
    14761 tgttgtgttt agcccattcg ttcgccatca atcgcgtcac acccatcacg ccgctttttg
    14821 atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac gcgatattga
    14881 taatcttgcc gccattgcct tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga
    14941 atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg aactcgagag
    15001 cgtcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga ccaaactccg
    15061 ctaccgcgcg atccagcagt gctggaatac catcaatctt tcgcagatcg gcggtcaggc
    15121 ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt tcaacgatgt
    15181 taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc tggcccagcc
    15241 cagtatcaca gccagtgacg accgcaactt taccttcgag agaaaatgca cttaaaatca
    15301 taacaatacc ttattcgttt atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc
    15361 catatcatca aagacctggt tttcaccgac catgccccag ataaaggtat aagctttggt
    15421 tccgacgccg gaatgaatcg accagctcgg ggagatcacc gcctgctcgt tatgcatcac
    15481 aatatgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat
    15541 attgaaatag aaataaactt ccatccggcg ctcgtgggtg tgacacggca tggtgttcca
    15601 caagttaccc ggagccagct ccgtcagccc catactcaat tggcaggttt ccagtacatc
    15661 cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg aggttatcgc ctaacgtgac
    15721 tggagatact tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca
    15781 attgtaataa aacttcgccg gagtgccggt atcaatactg gcaaagacaa cttcttttgc
    15841 acctttacca acatacaggg cgtcgcggtg accgatttca tagcattggc catcgacagt
    15901 aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaatagct
    15961 tacgccgagt tgtttaccaa cttccccgcc aacggaaacc gttttagtta tcggcataat
    16021 gccgccaaca ataattcggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa
    16081 taccttttca accaaaaatt cattgcgcag cccttgggta tccagcgttt ttgcgtgcgc
    16141 actgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat
    16201 agaaaacgat agtgaaacac tgtttcgttt tctctataaa gggatgatat gcccatgggc
    16261 tagggttttc aattaataat aaaacaatgt ttcattttta agttaaggat taaaaaaagt
    16321 gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca gaaaatgtat
    16381 gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg
    16441 aggaaatgca gagctattcg tcacgttcaa tggtcaatgc cacgccctgg cccccgccga
    16501 tacaaagcgt tgccagtcct ttgcgggcat tacgtttcac catttcatga accagagaaa
    16561 ccaggattcg gcaaccggaa gcgcctatcg ggtgaccaag tgcgatcgca ccaccattga
    16621 cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa
    16681 acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca cgctccaggc
    16741 aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg
    16801 caaatgcgcg aatgcgggcc agcacgggta aattcaacgc tcgtgctttg gcttcgctca
    16861 tcatcattac cgcagctgcg ccatcgttta tggatgatgc attacccgct gtcacagaac
    16921 cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg tcagtgcgtg
    16981 gctgttcatc ggtatcaaca accaacgtct gaccgttact ttgggtcatt accgggacga
    17041 tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg
    17101 cgtaagcatc ctgcaattga cggctgatgc catattcgcg agccagattt tcggcggtga
    17161 caccaatatg ataatcattg aaggcatccc ataacccatc atgcacaaga ctgtcaacca
    17221 gctggctatt gccaagctgt gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc
    17281 tcatgttttc ctggccaccg gcgatgacaa tatcagcctc gccacactgt atcgcctgag
    17341 tagccagatg cagtgcttta agtccggaac cgcagacgtc attaatcgtg attgcagaaa
    17401 cgctattagg cagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg
    17461 cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt
    17521 ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac
    17581 cgcgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa
    17641 cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat cttaattgtt
    17701 taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagtagtcag agtcataact
    17761 ggcacgtaag gtttgcaacc actaacccac caatagaggg gtagataggg cgttaatctc
    17821 ccatacttaa cctggtttat ggtaaattgc cctccatttt gtttaatttg tagatgatac
    17881 gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtggagttt cgcgctctac
    17941 ggcacagccg ttggtgcagg cacgctcttc cttcctattc agttaggttc ggcaggggct
    18001 gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc acataaagcc
    18061 ttatgccagt tcatcctctc atcgaaaaca tcagcaggtg aagggataac gggcgcagta
    18121 acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt
    18181 tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga acagctggca
    18241 aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatc
    18301 ctgaatctca tttttctgat gggacgccat gcaaccattc gggtaatggg ttttttggta
    18361 ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag ttggcaacct
    18421 gatctattaa cgacccaggt agagttcaat cagaataccc ttcaccagat atggatatcg
    18481 attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac
    18541 agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct
    18601 tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca
    18661 ccttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc gctttctatg
    18721 ctgccgaacg ccccagcatg gctgtcaatt tccgggatta ttgtcgcagt agttgcgatg
    18781 tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacgga ggtcgtcaaa
    18841 acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccgcgc actatcaatt
    18901 atgttggtat cgctgatcac cttcattgtt tgttgcatta acccgaacgc gatttcgatg
    18961 atttacgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgttgtca
    19021 acgtatctga tcccggcgct taaaccctgg cgttccatcg gaaatctgat tacgctgatc
    19081 gtgggtatcc tgtgcgtatc ggtaatgttc tttagctgat atccattgtg aatggttcaa
    19141 aattgctaca attttgaacc attccagttt tcataatgtt cattcgttat tattccgcac
    19201 tatcacttcc ctcatcatat cctagtatta tcagttagtt aaacacacaa agagatctga
    19261 acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat cttctggtca
    19321 ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc tgaaccaccc
    19381 tcaaccaggt aagattaaat cagtgaaaca taatcatatt taataacgag aaatgcattt
    19441 taaaatgtct taatcagaat tatagcaaat acagattaat cgcaattttt tccagcaaaa
    19501 ttacaaaatc atggtgtatt gacatcatca atcaccaatg aaattatgca attatatacg
    19561 gatagggagg ttcttaacat ggggctttgt agtcgttata aaagtcttac atgcaatagt
    19621 tgctcaatgc attgccaaat aatgccagaa gagtcaccgc gtttgcagta ctgcgcaaac
    19681 tcgtgttttt gtatgtggcc cgaagaaagt tcatatttta atcgtggtgt ggtagaaggt
    19741 atcttaacaa aaaaccacaa tgcgagatta agcggatata tttttgtcga tttttcagta
    19801 agttttttac gtctattcct tgaaaaagac tggattgact atcttgcaag tactgacatg
    19861 gggattgttt tagtcagcga ccgcaatatg caatcgttag ctaattactg gcgaaaacac
    19921 aactcagcaa tatctgctgt catatataat gatgatggtc ttgatgttgc caatgaaaag
    19981 atcagacaac tatttattgg tcgttattta tcatttacca gaggtaacac attaactcag
    20041 atggaattca ccataatggg gtatatggtt tctggttata atccatatca gattgctgaa
    20101 gttctggata tggatatccg tagcatctac gcgtacaagc aacgaatcga aaagagaatg
    20161 gctggtaaaa taaacgaatt atttattcgt tcacattcgg tccaacattg atactcaaca
    20221 atctgcaaga atcatactct gtttatacgg gcatttatgg aaatgcccgt attttataaa
    20281 taggccgtcc cctcaagagt aaacacatag tatttaaaac catcacacga aaaattcaga
    20341 agcattacga aatatgaact tacaaacaat agtggcataa atgttaacca tgttaattta
    20401 cataaagttt tacgttgcga cattaaagcc tcatttcaat catcatgata aatataaaat
    20461 taatatatat ttatgccgta aataccaata tacttagcaa actatgtgat ttccatttcg
    20521 attgatttag tgtttattga cgtatgtact ggattattaa cgataatatc gagttctggc
    20581 ctgagcaccg aaaattaata tcggtacata acgccgatct taacgtcgtt ctgacaacgc
    20641 cagccagtcg ttgtttatca cttctacttg aagcttttcc tgatgtggtt gcacaacaag
    20701 attttttcac cagagtctgg gaagaagaag gtatgcgtgt gcctactaac acgttatatc
    20761 agaacatatc cattatcaga cgcggatttc gcgctgttgg tgatactacc cactcgctta
    20821 ttgcaaccgt accgagaaga ggattcaaga tccataatga cattaacata caaaatcatg
    20881 taataaactc atcaacagac gcacatacac acaatgcccc acctgccata aaagttaatg
    20941 cgggatacaa agagagcatt ggtggcgcaa agaatttcaa taacaaaatc ctcaaacata
    21001 taaaaagtta tctaattatg ttgagcgcat ttgtcatagg cgcatattct gcatattggc
    21061 tatggaataa taatcaaccg aaaccatttt tcaaggatta caagactgtt gcggaaatta
    21121 atggctgcca ttttaatgta acagaagata caattgatgg gttgaaagag ttcgataaat
    21181 ataaaacacg gatactggat tcggggatta actgtaaaaa atatccgtgg ttatacttcc
    21241 ctcttgcaaa atcctcaccg gggatgattg ttatggcatg caataaaaac tataaccaac
    21301 atgaagtggc tggctgttta accctgtctt accgtgaggt taatcgtgat tgattataaa
    21361 aaaaacttat tatttatttt agtttttatc agtgggttta ttctcttcac tgtctattct
    21421 tacactgcag aaaaaatgat atataacgaa acatgcactg caaattgggt aatttttaat
    21481 gaccagggac gagcaaatct taccatcgac tttatgttta acaaaaaaaa taaaacagga
    21541 actgttgcgt tgagtggaac ctggcaacaa ggtaacagag aaagtaaatc aatacggcga
    21601 aatattgaat acacatggat tgaaaactat gacacagccc atttaacatc aaagaaagtt
    21661 aataagtttg agattatgga tcaagttgat gatgatagac ttgtacaact tattcctgat
    21721 ttttatgtct ttccagaaaa aagtgtaagc tataatatac taaagcaagg taagcatgct
    21781 tttattttga gcattggtaa cagagcaata atgcattgtg caaggtaaac taaaatagat
    21841 aacaaaaaga tggaacctcg ttaatcataa aacaataaaa ttaaatattc tcgcagtata
    21901 tggcagtcta aagcatcaaa gttttgatca acatctttca ttttagacat ctccttgcaa
    21961 tgtaaaatat catgtatcaa caacatctgg tgcaaatcca tgagtcgaac tcgacattca
    22021 tcttagctcg attattattc cttcgtacag tcgatgtaaa caatacagaa agaggattat
    22081 taagaaccag tttactcagt gaatcaatag aggaaaggat taacgtttct ttaaaagaat
    22141 taatttcatc atctgttaaa ctaaactcat cattgacaga tcgtgagata taactgtttt
    22201 taactttact ctttacgttg actttattga cagaattaac attcacatat cttgaattta
    22261 atgtccatga tgttgtttgc gtgagaacct tctcagcatt aaggaatttt ttaacggaat
    22321 ccttggtttc tttttcgagt ccgaatttac aatatcatgc atatagacca tttcatgaat
    22381 ctgcgaaaac tcaatgtcca ttgtatacct cacattttta ccgtgactcg atgttactgt
    22441 tcaataatca ccttccatca atactaaaat taatacccct aatgtgccga taacaaatat
    22501 agtcattcta cgtaacgtct ccataaggtg atatttgaca ttatcagaag ctgcgaattc
    22561 ggattttgct ctaatcaaat gagagaaatg taaaatgaag ccacgaaata ttaataataa
    22621 cctatcactg caaccattag ttcctgatca ggagaacaaa aataagaaaa atgaagagaa
    22681 atccgttaat ccagttaaaa tcacaatggg gtctggttta aattatattg aacaagaatc
    22741 tcttggagga aaatatctaa tacatgattt gtcaataaag atagcggata tttctgaaga
    22801 gataattcag caagcaatat tatctgctat gagcatatat aaattttcga taacagatga
    22861 tttaatgagt atggctgtaa atgaactcat aaaactgacc aaaatagaga ataatgtaga
    22921 cctgaataaa ttcactacta tatgcacaga cgttctatcc cccctcgtca ccagacataa
    22981 taaagaaaaa aaacaaacga cattctaccc ttcgcaaaaa tccccttttt aattttcatt
    23041 gaaaaaatgg caattgatac tatgaatcca ggtcatacac attaaggcat ttatgtaatg
    23101 tcgtaattaa gacaactaat aaggtgaata ttagtaaaac aactaatgat gttattattt
    23161 tgtagtaaac agtactttta ccaataatga aaaatatacc ataagcaatg caagcttaaa
    23221 accaataatt taaaaaagat aaatgtaaaa atattaatga gtcaatcaat aataatttag
    23281 catcacgaga cacatcacag aggaatatta tgagcacaga aacaattgaa atattcaata
    23341 atagtgatga atgggcaaat caactaaaac acctattatc gaaaggagaa aatctggcat
    23401 tactacatgg tttaactcct gatatccttg atagaatata tgcatatgca ttcgactacc
    23461 atgaaaaggg taatgtaacg gacgcagaaa tttattataa atttttgtgc atttatgcgt
    23521 tcgaaaatca tgagtatcta gaaggttttg catcagtatg tca
//
LOCUS       DAFATA010000067        23248 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFATA010000067 DAFATA010000000
VERSION     DAFATA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.068"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..430)
                     /gene="ulaR"
                     /locus_tag="LGJ20_003127"
     CDS             complement(<1..430)
                     /gene="ulaR"
                     /locus_tag="LGJ20_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBK2387152.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGG"
     gene            complement(538..1602)
                     /gene="ulaG"
                     /locus_tag="LGJ20_003128"
     CDS             complement(538..1602)
                     /gene="ulaG"
                     /locus_tag="LGJ20_003128"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK2387153.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1957..3354
                     /gene="ulaA"
                     /locus_tag="LGJ20_003129"
     CDS             1957..3354
                     /gene="ulaA"
                     /locus_tag="LGJ20_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK2387154.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            3370..3675
                     /gene="ulaB"
                     /locus_tag="LGJ20_003130"
     CDS             3370..3675
                     /gene="ulaB"
                     /locus_tag="LGJ20_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBK2387155.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            3685..4149
                     /gene="ulaC"
                     /locus_tag="LGJ20_003131"
     CDS             3685..4149
                     /gene="ulaC"
                     /locus_tag="LGJ20_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBK2387156.1"
                     /translation="MKLRDSLAENKSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            4163..4813
                     /gene="ulaD"
                     /locus_tag="LGJ20_003132"
     CDS             4163..4813
                     /gene="ulaD"
                     /locus_tag="LGJ20_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK2387157.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            4823..5677
                     /gene="ulaE"
                     /locus_tag="LGJ20_003133"
     CDS             4823..5677
                     /gene="ulaE"
                     /locus_tag="LGJ20_003133"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBK2387158.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            5677..6363
                     /gene="ulaF"
                     /locus_tag="LGJ20_003134"
     CDS             5677..6363
                     /gene="ulaF"
                     /locus_tag="LGJ20_003134"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBK2387159.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGEVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(6492..6767)
                     /locus_tag="LGJ20_003135"
     CDS             complement(6492..6767)
                     /locus_tag="LGJ20_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2387160.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDMQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            7094..7489
                     /gene="rpsF"
                     /locus_tag="LGJ20_003136"
     CDS             7094..7489
                     /gene="rpsF"
                     /locus_tag="LGJ20_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK2387161.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            7496..7810
                     /gene="priB"
                     /locus_tag="LGJ20_003137"
     CDS             7496..7810
                     /gene="priB"
                     /locus_tag="LGJ20_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBK2387162.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            7815..8042
                     /gene="rpsR"
                     /locus_tag="LGJ20_003138"
     CDS             7815..8042
                     /gene="rpsR"
                     /locus_tag="LGJ20_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK2387163.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            8084..8533
                     /gene="rplI"
                     /locus_tag="LGJ20_003139"
     CDS             8084..8533
                     /gene="rplI"
                     /locus_tag="LGJ20_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK2387164.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(8645..9616)
                     /locus_tag="LGJ20_003140"
     CDS             complement(8645..9616)
                     /locus_tag="LGJ20_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBK2387165.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(9669..10970)
                     /locus_tag="LGJ20_003141"
     CDS             complement(9669..10970)
                     /locus_tag="LGJ20_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBK2387166.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(11013..11489)
                     /locus_tag="LGJ20_003142"
     CDS             complement(11013..11489)
                     /locus_tag="LGJ20_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK2387167.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(11510..13591)
                     /locus_tag="LGJ20_003143"
     CDS             complement(11510..13591)
                     /locus_tag="LGJ20_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBK2387168.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(13588..15129)
                     /locus_tag="LGJ20_003144"
     CDS             complement(13588..15129)
                     /locus_tag="LGJ20_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBK2387169.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(15139..15915)
                     /locus_tag="LGJ20_003145"
     CDS             complement(15139..15915)
                     /locus_tag="LGJ20_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBK2387170.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEIEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(15925..16767)
                     /locus_tag="LGJ20_003146"
     CDS             complement(15925..16767)
                     /locus_tag="LGJ20_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK2387171.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASKMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(16777..17568)
                     /locus_tag="LGJ20_003147"
     CDS             complement(16777..17568)
                     /locus_tag="LGJ20_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBK2387172.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            17778..18416
                     /locus_tag="LGJ20_003148"
     CDS             17778..18416
                     /locus_tag="LGJ20_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK2387173.1"
                     /translation="MYLDTSHAQNLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIREKERELDISGLSPVIAIEKIIDFTWDYYINNP
                     WFLKIVHSENQSKGVHYKKSKRLAEINHAHLQSMASLLEEGQRQKTFKKDIDPLQINI
                     NIAALGAYYLINQYTLGMVYHISMVSPEALEARRRVIKETILSWLLVNPARG"
     gene            complement(18417..19046)
                     /locus_tag="LGJ20_003149"
     CDS             complement(18417..19046)
                     /locus_tag="LGJ20_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBK2387174.1"
                     /translation="MPGRFELKPTLVKIWHAPDNFRLMDPLPPMHRRGIIIAAIVLII
                     GILLPGDETRQMPVVTREAQLDMQSPPPGEAQLQAHLVTPQNDPDQVAPVAPEPIQEG
                     QPEEPPVAETQSFQPESGIDQQWRTYRVEPGKTLAQLFRDHNLPPTDVYAMAQVEGAD
                     KPLSNLQNGQIVKIRQNANGVVTGLTIDTGNNQQVLFTRQPDGNFIRAR"
     gene            19265..19885
                     /gene="fklB"
                     /locus_tag="LGJ20_003150"
     CDS             19265..19885
                     /gene="fklB"
                     /locus_tag="LGJ20_003150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK2387175.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            20194..21606
                     /gene="cycA"
                     /locus_tag="LGJ20_003151"
                     /gene_synonym="dagA"
     CDS             20194..21606
                     /gene="cycA"
                     /locus_tag="LGJ20_003151"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBK2387176.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(21651..22313)
                     /gene="ytfE"
                     /locus_tag="LGJ20_003152"
     CDS             complement(21651..22313)
                     /gene="ytfE"
                     /locus_tag="LGJ20_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBK2387177.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22421..>23248)
                     /locus_tag="LGJ20_003153"
     CDS             complement(22421..>23248)
                     /locus_tag="LGJ20_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2387178.1"
                     /translation="PIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTG
                     APVSTMIIGTLPVVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGL
                     PDFDWARYTSGIVLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLI
                     GYLVACYWLNTQTPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVIL
                     GPLIVFETLAGLLYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
BASE COUNT         5661 a         5822 c         5964 g         5801 t
ORIGIN      
        1 gtccacccat aatgatcacg ctgtcatgtt cttgatcgat caggtaattt gccagcggta
       61 gataattagt gatgatttgc actggcttgc cacacatttc ccgcccaagc agaaacgcgg
      121 tggagccgca gttgatgact acgctttcgc ccggattaac cagctgcgag gctgctttag
      181 cgatacgtac tttttcatcg tgattctgcg cctgatgcag attcatcggc gtccagcgcg
      241 ggcgctgctg ggtaatagct tctgcgccat tgcgcacctt tttcagtttg ccgctttcgt
      301 caagtttatt gatatcgcgt cgcgcagtgg caggcgaaat tcccagacgc tcaacgactt
      361 tctcaacggt cacaaagccc aattgtgcga gcatttccag gaggatttga tgtctttgtg
      421 cttcagtcat gagctattcc gataaaattt gattttttta gatgatattt gaaatagcca
      481 ggaaatacta cgccggatag cgcgaacttc tccacgctat ccggtataaa cgacagatta
      541 caggaatgac ttgaacggca gatccggttc aatagtgaag caatcatcga aaccgcgcgg
      601 atagtggtac tcgaagttgt ctttatccag cggccaggta aatttgccgc caacctgcca
      661 gataaacggc ttgaagccat acttcagacg atcttttttc atctcccaca gcacgcggat
      721 ctcttgcgga tcggcctgga agtttgacca gatgtcgtgg tggaacggga taaccacttt
      781 cgcattcagg gcttcaccca tacgcagcat atcggcgctg gtcattttgt cggtaatacc
      841 gcgcgggttc tcaccatacg atcctaacgc cacatcgatc tgatgttcgt taccgtgctt
      901 cgcataatag ttagagtagt gggagtcgcc gctgtgatac agggagccgc caggcgtttt
      961 gaacagatag ttcaccgcgc gatcgtccat gccgtccggc agtacgccag ccgctttttg
     1021 atcggcaggc agggtgatca gtgcagtacg gtcgaaagcg tcaagcgcat gaatttcaat
     1081 gtctttcact tttacaacat cgcccggttt gaccacgatg caacgctctt tcggtacgcc
     1141 ccagccaatc cacaaatcaa cgcaggtttt cggtccaatg aatggtacgt catcagcaca
     1201 gttctgcatc acggcagcag cgacgttaac gtcgatatga tcgttgtgat cgtgagtcgc
     1261 cagtaccgcg tcgatctggc gaatcgcaaa cggatcaaga acaaacgggg tggtgcgtag
     1321 gtttggctgc agttttttca cgccagccat gcgctgcatc tggtgaccct gtttcattaa
     1381 cgggttaccg tgactttgtt tgccagtgcc gcaccagaaa tcaacgcaaa cgttggtgcc
     1441 accttctgat ttcaaccaga tcccggtgca gcctagccac cacatcgcaa atgtgccagg
     1501 ggcgacctgc tcttgttcaa tttcttcatt cagccagcta ccccactccg ggaaagtgct
     1561 caggatccag gattctcggg tgatactttt cactttactc atcgccattt accttcatga
     1621 tagttcaatt cgaatcaata tgtgattggt tttgattaat cctgacacta ttttttcagg
     1681 aaggcaatga ccattttttg actttttgcc agggaagtgg ttgttgattt tgagtgtgga
     1741 aagatttaat ggaatgtgta attcaattaa ctaaatgaat ttaaatgaat agttgtttcg
     1801 ttgtgtgaat cccattctat ccatgtggaa ttatttgcgg gtcgcgtcac atttaatcat
     1861 aaataatctt gttgtgatta cttttgaaaa ttagagtgag tgcacaacat tccgggtgtg
     1921 tggaataccc ggttacctct tcttcaggag atcgttatgg agatcctcta caacatcttt
     1981 accgtgtttt ttaaccaggt catgaccaat gccccgttgt tgctgggtat tgtgacctgt
     2041 ctgggctaca tcctactgcg caaaagtgtc agcgttatta ttaaaggcac gattaaaacc
     2101 ataattggtt tcatgttgtt gcaggcaggg tccggcatcc tcaccagcac cttcaaaccg
     2161 gtggtggcga aaatgtccga agtctacggc attaacggcg caatttccga tacttacgcg
     2221 tcgatgatgg caaccatcga ccgcatgggc gatgcctata gctgggtagg ttacgcggtg
     2281 ttgttagcgc tggcgttgaa catctgttac gtgctgctgc gtcgcattac cggtattcgt
     2341 accatcatgc tgactggtca tatcatgttc cagcaggcgg ggctgattgc cgttacgttg
     2401 tttattttcg gctactccat gtggacgacc attatctgca cggcgattct ggtttcgctc
     2461 tactggggca ttacctccaa cataatgtac aagccgactc aggaagtgac ggacggctgc
     2521 ggtttctcca tcggtcacca gcaacagttt gcatcatgga ttgcctataa agttgcgccg
     2581 ttcctcggca aaaaagagga aagcgttgaa gacctcaaac tgccaggctg gctgaatatt
     2641 ttccacgaca acatcgtctc cacggcgatt gtgatgacca tcttctttgg tgccattctg
     2701 ctctccttcg gtatcgacac cgtgcaggcg atggcaggca aagtgaactg gacggtgtac
     2761 atcctgcaaa ctggtttctc cttcgcggtg gcgatcttca tcatcactca gggcgtgcgc
     2821 atgtttgtgg cggaactctc tgaagcattt aacggtatct cccagcgcct gatccctggt
     2881 gcggttctgg cgattgactg tgcggctatc tatagcttcg cgccgaacgc cgtggtttgg
     2941 ggctttatgt ggggcaccat cggtcagctg attgcggttg gcatcctggt cgcctgcggt
     3001 tcctcgatcc tgattattcc aggctttatc ccgatgttct tctccaacgc caccatcggc
     3061 gtgttcgcta accacttcgg tggctggcgt gcggcgctga agatttgtct ggtgatgggg
     3121 atgatcgaaa tctttggttg cgtctgggcg gtgaaactta ccggtatgag tgcctggatg
     3181 ggtatggcgg actggtcgat tctggcaccg ccgatgatgc agggcttctt ctccatcggt
     3241 atcgccttta tggccgtcat cattgtaatt gcactggctt atatgttctt cgctggccgc
     3301 gcactgcgcg cagaagaaga tgcagaaaaa caactggcag aacagtctgc ttaataagga
     3361 gttttgatta tgaccgtacg tattctggct gtgtgtggca acggacaagg cagttccatg
     3421 atcatgaaga tgaaagtgga ccagttttta acccaatcaa acattgacca cacggtaaac
     3481 agctgcgcgg ttggcgaata caaaagcgag ttgagcggcg cggatatcat catcgcttct
     3541 acgcacattg cgggcgaaat caccgtgacc ggcaacaaat acgtggttgg tgtgcgcaac
     3601 atgctctctc ctgccgactt tggcccgaaa ctgctggaag tgatcaaaga acatttcccg
     3661 caggatgtga agtaaggacg cgccatgaaa ttacgtgatt cgctggctga aaataaatcc
     3721 atccgcctgc aggccgaagc aaatacctgg caggaagcgg tgaaaatcgg cgttgacctg
     3781 ctggtagcgg cagatgtggt agagccgcgt tactaccagg cgattctgga tggcgttgaa
     3841 cagtttggtc cttatttcgt tatcgctccg gggctggcaa tgccgcacgg gcgtccggaa
     3901 gagggcgtta agaaaaccgg tttctctctg gtaacgctga aaaagccgct ggaatttaac
     3961 cacgacgaca acgatccggt ggatatcctc atcaccatgg cggcggttga tgccaacact
     4021 caccaggaag tgggcatcat gcagatcgtc aacctgtttg aagatgaaga gaatttcgac
     4081 cgtttacgcg cctgccgtac cgagcaggaa gtactggatc tcattgaccg caccaacgcg
     4141 gcagcttaag aaggaattga acatgtcatt accgatgttg caagtcgcgc tggacaacca
     4201 gacaatggat agcgcctacg aaaccactcg cctgattgcc gaagaagtcg acattatcga
     4261 agtgggtacc attctgtgcg tgggcgaagg cgtgcgtgcg gttcgcgatc tgaaagcgct
     4321 ctacccgcac aaaatcgtac tggctgacgc caaaattgcc gatgcaggca aaatcctttc
     4381 gcgtatgtgc ttcgaagcca acgctgactg ggtgaccgtg atttgttgtg cggatatcaa
     4441 caccgccaaa ggtgcgctgg acgtggcaaa agagtttaac ggcgacgtgc agatcgaact
     4501 gaccggttac tggacctggg aacaggcgca acagtggcgc gatgcaggta ttcagcaggt
     4561 ggtttatcac cgcagccgtg acgcgcaggc cgctggcgtg gcatggggtg aagccgatat
     4621 cactgcgatc aaacgtcttt ccgatatggg cttcaaagtc accgtcaccg gtggcctggc
     4681 gctggaagat ctgccgctgt tcaaaggcat tccaattcat gtgtttatcg ccggtcgcag
     4741 catccgtgat gccgcttctc cggttgaagc cgcgcgtcag ttcaaacgtt ccatcgccga
     4801 actgtggggc taaggagcgg atatgttgtc caaacaaatc ccgcttggca tctatgaaaa
     4861 agcgctcccc gccggggagt gctggctgga acgcctgcga ctggcaaaaa cgttgggctt
     4921 cgattttgtc gagatgtcgg tagatgaaac tgacgagcgc ctgtcgcgcc tcgactggag
     4981 ccgcgagcag cgtctggcgc tggtgaatgc gattgttgaa accggcgtgc gtgtgccgtc
     5041 catgtgcctt tctgcccatc gtcgttttcc gttgggcagc gaagatgacg cggtgcgggc
     5101 gcaggggctg gagattatgc gtaaagccat ccagttcgcc caggacgttg gcattcgcgt
     5161 gatccagctg gcgggctatg acgtttacta tcaggaagcc aataacgaaa cgcgtcgtcg
     5221 tttccgtgac ggcctgaaag agagcgttga gatggcaagc cgcgcgcagg tgacgctggc
     5281 gatggagatc atggattatc cgttgatgaa ctccatcagc aaggcgctgg gttacgcgca
     5341 ctatctcaac aacccgtggt tccagctcta cccggatatc ggcaacctgt cggcgtggga
     5401 caacgatgtg cagatggagt tgcaggccgg aatcgggcat atcgtcgcgg tacatgtgaa
     5461 agacaccaaa cctggcgtct tcaaaaacgt gccgtttggc gaaggtgtag tggatttcga
     5521 acgttgtttc gaaacgctca aacagagtgg ctattgcggg ccgtacctga ttgagatgtg
     5581 gagcgaaacg gcggaagacc cggcggcaga agtggcgaaa gcgcgtgatt gggtgaaagc
     5641 gcgcatggcg aaagcgggca tggtggaggc ggcataatgc aaaagctaaa acagcaggta
     5701 tttgaagcca acatggagct gccgcgctac gggctggtga cctttacctg gggcaacgtc
     5761 agcgctatcg accgcgaacg cgggctggtg gtgatcaagc ccagcggcgt tgcctacgaa
     5821 accatgaaag cggccgatat ggtggtggtt gatatgagcg gcaaggtggt ggaaggggag
     5881 tatcgcccat cttccgacac tgcgacgcat ctcgaactct accgtcgtta cccgtcgctt
     5941 ggcggcattg tccataccca ctccactcat gccaccgcat gggcgcaggc ggggctggca
     6001 attccggcgc taggcaccac gcacgccgac tacttctttg gcgatatccc gtgcacgcgc
     6061 ggattaagcg aagaagaggt gcagggcgag tatgaactga acaccggcga agtgattatc
     6121 gaaacgctgg gtgacgccga gccgctgcat acgccgggga ttgtggtgta tcagcacggg
     6181 ccgttcgcct gggggaaaga tgctcacgat gcggtgcata acgcggtagt gatggaagaa
     6241 gtggcgaaaa tggcgtggat tgcgcgcagt attaacccac aactgaatca catcgacagc
     6301 ttcctcatga ataaacactt catgcgtaag cacggtccta acgcttatta cgggcagaag
     6361 taaaacacgc gctgcggaaa tttccttctt cgggagataa ctggtctaat tccgcagccg
     6421 tttttcaaaa aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt
     6481 tatacctggt gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt
     6541 tcccgcatca gaattacgtg gtagtacgtt gcgccctgcg catctgtttc ttcatttaat
     6601 gcctgacgtg cttcactttc agtggcgaaa ttatgattga tataaatcac gcctaagctt
     6661 tgcatatcgt ccatatttct ggcctggtgg ttattgattt caatggctgc ccatgtattt
     6721 gcacttagca aaagcacagc cagaagggct aaaactcgac tgaacataga tacctcctcg
     6781 acggctgact ttgtgtgctc tcctctgtga tgatcttctg atttaatttt aatcaatgat
     6841 aaagaagttg atggtgacca tttctgatgc agttgttcaa aaaaacacca tgatgaagtg
     6901 tgatgaactc caaatcagcg gtttagaggt taattgcgaa aggggagatt tatttcggct
     6961 ctgcccttga gtttagcggg gcatacaagt actataacgc gtcatttttc agccgacctt
     7021 taacacgttc cttgcctccc cgggattcgg ctgacccaga caggaggctg aataatccgt
     7081 aaggagcaat tcgatgcgtc attacgaaat cgtttttatg gtccatcctg accagagcga
     7141 acaggttccg ggcatgatcg agcgctacac tgctgccatc actggtgcag aaggcaagat
     7201 ccaccgtctg gaagactggg gccgccgtca gctggcttac ccgatcaaca aactgcacaa
     7261 agcacactac gttctgatga acgttgaagc tccgcaggaa gtgatcgatg agctggaaac
     7321 taccttccgc ttcaacgatg ccgttatccg cagcatggtt atgcgtacca agcacgctgt
     7381 taccgaagca tctccgatgg ttaaagcgaa agacgagcgc cgtgagcgtc gcgatgattt
     7441 cgcaaacgaa accgctgatg atgctgaagc tggggattct gaagagtaat ttctgatgac
     7501 caaccgtctg gtgttgtccg gcaccgtgtg caggactccc cttcgaaagg tcagtccatc
     7561 aggaattcct cactgccagt tcgtgcttga gcatcgttct gtgcaggagg aagccggctt
     7621 tcaccggcag gcatggtgtc aaatgcccgt tattgttagc ggacacgaaa accaggccat
     7681 tactcacagt ataacggtcg gcagtcgcat aaccgttcag gggttcattt catgccacaa
     7741 ggcaaagaac ggactgagca aaatggtttt gcatgccgag cagattgaat tgatagattc
     7801 tggagactag ccatatggca cgttatttcc gtcgtcgcaa gttctgccgt ttcaccgcgg
     7861 aaggcgttca agagatcgac tataaagata tcgctacgct gaaaaactac atcaccgaaa
     7921 gcggtaagat tgtcccaagc cgtatcaccg gtacccgtgc aaaataccag cgtcagctgg
     7981 ctcgcgctat caaacgcgct cgctacctgt ccctgctgcc gtacactgat cgccatcagt
     8041 aatcggtcac ggtccattaa tacgactttg agaggataag gtaatgcaag ttattctgct
     8101 tgataaagta gcaaacctgg gtagcctggg tgatcaggta aacgttaaag cgggctatgc
     8161 tcgtaacttc ctggtaccgc agggtaaagc tgttccagct accaagaaaa acattgaatt
     8221 cttcgaagca cgtcgcgctg aactggaagc taaactggct gaagttctgg cagctgctaa
     8281 tgctcgcgct gagaaaatca atgcactgga aactgttacc atcgcgtcta aagctggcga
     8341 cgaaggtaaa ctgttcggtt ccatcggtac tcgcgacatc gctgacgctg taactgcagc
     8401 tggcgttgaa gtggctaaga gcgaagttcg tctgccgaac ggcgttctgc gtaccactgg
     8461 cgaacacgaa gtgagcttcc aggttcacag cgaagtattc gcgaaagtga tcgtaaacgt
     8521 agtagctgaa taattcgtta ttcaacgaga cgtaaaaacg ccgaccattg gtcggcgttt
     8581 tgcttttgta gcgagcactt gcagatatcg ataagggcca caataagcag ccctacgaag
     8641 ataattactt agctgcctga atatcagcca gcattggggc aagggctggg ttgtccttag
     8701 cgaagtttgc gtacattggc tgtactttct cgataaacgg agctttatct acctggtaga
     8761 ctttcacgcc cattttatcc agtttcgcaa attcttgttt ctcttcctcg ctccacaagt
     8821 ctttcatata gctcattgat tctttagctg cttttaatat aactgcctga ttttccgggc
     8881 taagtttatc aaacgcagca ttgctcatca gaaccacatc gggaaccata gtgtgttcgt
     8941 caaggctgaa ggattttgcc acttcaccgt gacgcatggt ggtcagcgcc atcacgctgt
     9001 tttctgccat atcgaccaca ccctgctgta aggctgtata caactcgccg taatccagtg
     9061 gcgtcgggtt accgcccatg actttgatca tttcaactgc gccagggctt ggctggacac
     9121 ggactttcat gcctttcaga tcgtctggtt tcagtactgg tttattgcca tagaaactgc
     9181 gggcgcctcc atcataaaaa gtcagcccga aataaccttt gcttttcgtt gagtcgagga
     9241 tttttctccc gatatcgccc tgcatgacct gataataatg atcacgatcg cggaacaaat
     9301 aaggcaggct aaataattta taggaatttt caaatgcttc catttctgcc gcattggttt
     9361 ttaccagtgg aatagcgcca gaacgaatca gctccattgt ttctcgttga gtacccaatg
     9421 ttccatttgg ataaatacga atagtaatat caccgttaga gtactctttg ctctttttcg
     9481 caaagaactc catcgcttta tgaacaggat gagacttatc ctgattatga ctcagtttta
     9541 atgtcactgc ccaggactgg cttgcggata acataagaac agcaccgata atgccagcgg
     9601 atacttttat tttcatagat gttccttttc taataaatat ggcggcaggt gccgctagtg
     9661 aatgcgcatc agtaacccag tgctcttggc aatgccatac tgatttgcgg aatataggtc
     9721 accattgcca gcaccaatac catcacggca tagaatggca gcataggttt gataatttta
     9781 ttaatcggta atttagatac actacaaccg acaaataaca cactgcctac aggcggagta
     9841 caaataccga tacacatgtt cagcaccatg acaataccaa agtgaatcgg gtccatgccc
     9901 agttcggtga caaccggtaa aaatatcggc gtaaaaatca gtaccgccgg tgtcatatcc
     9961 ataaaagtgc cgataatcaa caggataata ttgataataa gcaggattat tattgggttg
    10021 tcagaaatag tgttaagcaa atcgagaatc aggaagggaa cgtcagcgtt tgacattgcc
    10081 catgacattc ccatcgaaga accaattaat aacaagacaa ttgcagtggt aataacggat
    10141 tccagaaata ttttaggtaa atctttaact gagatttcac gataaagaac cagtgcaagg
    10201 aaaagggtat aaattaccgc aattgcggag gcttcggttg gagtaaaaat accgcttagt
    10261 acgccaccca taattaacac aatcaggaaa atactgggga tagccatcca tatcgcaacg
    10321 ccagcctggc gcaaagtcgg acgttctggt tttggataac cgcgacgtac agcgataata
    10381 ccggctatta ccatcagtgc cagacccagc aaaatccccg gaacataacc cgccaggaac
    10441 agtgcggcaa tagaagtacc accactgacc agtgaataga cgattaacgt attacttggt
    10501 ggaataagca aacccgtcgg ggcagaggca acgttaaccg cagtactgaa tgccgggtcg
    10561 tagccctctt taacctgttg cggatgcatg acgccgccca tcgcagccgc cgaagccacg
    10621 gctgaaccag aaattgcccc gaacagcata ttggcgagaa tattacagtg ggcaagtgag
    10681 cctggcagac gaccgccgag aatacgggca aagttgatta atcgtcgcgc tattcctcca
    10741 tgattcataa tgttgccaga caagataaaa aatgggattg ccaacaacgt aaaactatct
    10801 aacccggagg ctaacttttg ggaaaccaca ctcaatgctg agttgaaatc gagttgtgtg
    10861 gaaattgatg ccagtgtcga taaaccaatg gcataaacaa taggtacacc taatgccaac
    10921 ataaaaaaga aggcaccaaa taacacaata atcggtaacc agtaatccat aatttctcca
    10981 ttcctactat ggttggttgt tgtttgttat ctttagagtg gttgtttaag ttcggttaaa
    11041 accatatcta tggtgtatat aataataagt acaccactaa tcgggatgct catatagata
    11101 tatcccattt gccagcctaa agcgggcgag gtctgacctg aatcgaatgt atccatcgtt
    11161 aatagcccac caccatatac cataatgatg gttgcgaaga taataacgat aatctgaata
    11221 cacaaaaata aatgacgtcg ggcttttcct ttcagcatca taggtagcag gtcaatcgcc
    11281 agatgccggt tttgccctgc ggtgtatgcc ccaccaaaaa gtccaagaca aataaagagc
    11341 aaacggctga gttcatctgt aaaaatactg gggctagtaa atataaatcg tgcagccacc
    11401 tgccacgtca cacatgccac gatgatcgcc aaaatggtaa tgttcaatgc tgcaagcaga
    11461 gcattaatag atctaattat atttcgcata gtgattaacc tttgcaatat cattcatcct
    11521 gtggctggta aattgtccca ttaagaaaac caggccagtt tttttcaaac caaatacctc
    11581 ggataatagg aaactcacga acaggtgttt ctctgacgtg aaagataccg ttgcaataat
    11641 gaataagtag tttatctttt ttatcctcag tacgctctga acgaaagcgc caggcatcac
    11701 aaaaaccatt tcgtgtttgc ggaatagaat ttccatccct gtcgagaata atacgtgctc
    11761 cccgactacc accacctgcg tcaatataat gcgttaattg cgttaatacg gcggcagaag
    11821 tcagtgccat atgccgccac ataaacagct cggcaacttc acccacatgt ttgatagcca
    11881 atccatgccg ttggacaaat tcgctcaata gcagggcatc gcgagtggcg cgtcggactt
    11941 tatcggcatg gcaaataaat cccgcatggt cagacattcg tgcctgaatt ttttctctca
    12001 caaccgacaa cggcatcccg gtcccgttat cgtgcgcctg agtaatgatc tctcttatag
    12061 aagccagtgt aggagctacc agctgtgcta tatctccatc aatattccgc ttttgcgtgc
    12121 aaccaataaa acgtgccaga cgaacagcaa aaacctgccc ggcattcaat gctgcaccac
    12181 cagggcgagt gacgccgtgt gtgccagcaa cttcccccac ggcgaaacag ccgggcaggg
    12241 aggtttgtcc ccagatatcc acttcaatgc cgccattcat atgctgatta ttcatggcaa
    12301 actgcaaagg ctgtgtggtg agatcgtaac cgtgcatctt atacagcgag atagacagcg
    12361 gattcattcg ttgcagccgt tcgatgggcg atggtgccag agcgtcgtta ttttccagat
    12421 acgcgcgaac gtcgtcgtcc agtcgatcta atgagaacgg caggtcaccc ggcaccgctt
    12481 caggattgcg attgaaatcc ataaatacct gacgccctga ttgctgctct tgcgctactg
    12541 ccatatctaa caagctggag ccaaaatcca tcacccgcgt ggcgtggaac ggccactgat
    12601 agcctttacg gaaaatgttt gaagccagct cctgggtggt gcgatagtaa tccgcgagga
    12661 agttatactc gttaccctca gcatccacgg aatagatata cgggatcacc tgtacatagg
    12721 tgccggataa attccacgga aacgtgctac gcggcgtgcc tataccaaac tggctttcgg
    12781 tcagattggt tagcgtcaga ccttcctcca gcgccagccc cagcgagccg aagcatttat
    12841 gtggataaac actgtcgcga taaagctcgc caggccctcc tgttgccagt accacattgg
    12901 gcgcagtcac aattgccagc ccccatgggt tatgggcgcg atgaccggtc gcgaggattg
    12961 cccctgccac gcggtcttcg ccgttttcgt cacgctgatg cagcagttta atcactgttg
    13021 cactggtcaa cactggaatg gcgaggcgct gtacttcttc caacagcact ttcaccatta
    13081 accttgaggt acgcggccca cacgaggttg cacgcccggc ttcgtcatgg tcggtttgat
    13141 aacgaagaat cgcgccatag cgatcttccg gtaattccag accaagatat tgcaacccgc
    13201 ctagagtgtg cagagaacct accgcttcga cataagcggt gtcgtgatcc atcgccccac
    13261 cgctcgccag tgcctctgcc agtttggtga agttgtcgcc gttgcccgcg gtagcggcgg
    13321 taaatagtgt ctgtttatcg gaaccagaac atgccgacgt ccccatatat aacccggcgg
    13381 tagcgatcag cacattttgc tggcggcgtt taagttcaac tgccgcacgt aatcctgctg
    13441 caccactgcc gactaccagg gcatctgttt gccagagtgg gtaatcaaca ccgtctaccg
    13501 tgacaaccca tgcgggctct gcctgtgcaa aaccctttgg gaaagtaacg tgcgtcagcg
    13561 catcaaggat ggtgtcatca agctgactca tgcatcctcc atcggcgtaa acaggctact
    13621 ttccatcagg cgtgggtgct tgataattgg acggaaagcc atatacggca gaatgtcgcg
    13681 ctcaatctcc acacccggcg cgatttcaat taattcaacg ccttctgccg ttaactggaa
    13741 tactgcgcgt tcagtgatat acaacacttc ctgaccactt tgctgtgcgt aggcggcgct
    13801 aaaagtgatt ttttccacct gggtaaccag tttagggatt tgacctgact gggtgatatg
    13861 caagccatct ggcgttacat caatcttgct acctttggcg tcgaacgtgc cgcagaacac
    13921 cactttacgg gcgttttgcg caatttcgag aaaaccgccg ggaccaatga gattgccatt
    13981 gagatgggat acgttgacgt taccgtactg atccatctct cccatgccga ggaaggtgat
    14041 atcgattcct ccgccactgt agaactcaaa ttgatcgagt gatggaataa tggcatcggc
    14101 gttgcgggcg cagccgaaaa acgcatcatc cagcatcata ccgttatgca cgccttgctc
    14161 gacgctcagc cataaacttt ggtaaggcac gccttcgcgc agcgctacgc ctggaatacc
    14221 cccaggaata ccaaagccaa agttggttga agcgccagct accagttcgc gtgccgcccg
    14281 acgggcaatc aggcgacgta ccggatgact aaccagttca atggcgtcgt tagaactcag
    14341 acggcgatgt tggccgctga tggtcaggtc gtaaccgcca agataggttt gtggttgttc
    14401 gcggtgttca acgatgccat caaccagaat gcccggtaat ttgacccggc gtggttcaat
    14461 cgcgccagct tccagcacat cgcgtacctg tacgaacact ttgccgccgc tgttgtgtgc
    14521 tgccagcgcc atggaatagc tatccatatc aatcgcttct tcttcaaggc tgacgttgcc
    14581 tcgaggatcg gcataagtgc cacgcaaaat cgcgtaatcc actttgaaag ggcgataacg
    14641 aagtttggtt tcaccgtcga tttcaatcag ttctaccagg tcgtcggtag tgcattcatt
    14701 cgacttaccg ccgccattgc gtggatcaac aaacgatccc agcccaacgt gggtaaaaag
    14761 ccccggacgt ccggcgccga tctcccgtag cagtgcctga atcacgccac caggaaagca
    14821 gtaggcttca atcgtgttat tttttaccag cgcctgcatt ttgggcgacc aggtaaagtg
    14881 cccggcaata atgcgtttga gcatctctgc gtgcgcaaaa cggttagtgc ctttgcagtc
    14941 gcgatcgcca atacccagcg agtggattag cgtcaggtcg cgagggtgtc cggtttgcag
    15001 gaaacgcgct tcaatagcag ccagaatatg gtcggcttcc accatgccgc cgccgttacc
    15061 gctaatggca atggttgcgc cgtcctgaat ttgcgccgcc agtgcctcgg cggttgttat
    15121 cttacgcata caatttcctt attgattatg gaagctcacg ctactttttt cggcaaaggc
    15181 gcgcgtcgcc tgctggcaat cctgagtggc ggacattaat ccggccagta gctggtgcgg
    15241 gtaatcggca tgtttatttt ccagtgccag aatcagttgc ttaatatgcg ccagtgccac
    15301 cggaccgcat ttttccagct gcgctaactg gttcgccatc cagctttcca cctgttctat
    15361 ttctaccact tcatcaatta aattcgccgc ctgggcttcc tgcgccgtca ggcgtttacc
    15421 cagcatcagc atctggcgac cgaccaccgg accaacctga ttaagcacac gttcaatgcc
    15481 catccagcca ggcaccatgc cgagcatcac ttccgggtta gagaatttcg cgccggggcg
    15541 ggcaatacga atatcgcagc acaaggccag ttcaatacca ccgccgatgg tatgaccgtt
    15601 gagattcgca acggttaatt ggggtaagtt tcgcaggcga ttaaaaacgt cattaccgcg
    15661 tttaatccat tttctgccca tatctaatgg cgaataggcc gaccacgatt taatatcgcc
    15721 gcccgcacaa aagaatttat cgccggttgc cgttaattcg actaatcgta atgtggtgtc
    15781 gttttcaatt tcatttaaat aatggtcgag agattcgatc atctcttcgt taatagcatg
    15841 acatttatct tcacggttca atgtcaggcg gcaggaagcc gccactctgc tgaacagaac
    15901 gggttggtca ctcatgatca ttccttaaca ataatgcgcg atgcgatcga cgctaatatc
    15961 cagcgtgctg ttaatatctt tctgccacca gtaaggcgag aatatttcca gttcaatagc
    16021 accattaaat ccggcgtttt caaccagccg acgaattgac ggaatattga tgacgccatc
    16081 tcccggcata ccacggtcat tgaccagatc agtggtcggc actaaccagt cggaaacatg
    16141 aaacgcgagg atgcgttttc cggcacgcag gatctgactg gcgagatccg ggtcccacca
    16201 aacatggtag acatctaccg ccacgccgag gccaaattcg ccatcgggat cgagttcatc
    16261 acaccagtcc agcgcctgac gtaacgtgca cagacaggaa cgatcggctg cggtcatcgg
    16321 gtgcagcggc tccagtgcaa ttggcacgcc gacatctttc gaatgtggca gcaactggcg
    16381 aatcccttgt ttcacttgtt cccgcgcttc atatagatct ttagtgcccg ttggcagacc
    16441 gccaaccacc tgcatataac aggcggcatt cagtacagcg gcgtcgtcca gcgcccggcg
    16501 gttatcgtca atcgccagtt tgcgctccgc cagcgtcggt gcggtgtagt aggtgctacg
    16561 gcacaaaccg gaaacgttca tcttactggc ggcaagctga cgggcgatct gctggagatc
    16621 ttcgccctgc aactccctgc gccagggggc aattgcgcca ataccgcgtg cagcgcaggc
    16681 atcaataatg gcgggtagcg gcgttttata gcccagtgtt gcggtattga tactgaataa
    16741 atccgggcgt tgtaacagat ctctcatgat tcagcctcag aagcgcggca tacttaagcc
    16801 gccgtcgatg gcgaccgcct gaccacaggt gtaaatcagt ttaccttccg ccatcgcgcg
    16861 gacggtggaa gcaatatcag ccggataacc ccaacgtccc cacggcacca gacctttggc
    16921 aatcagttcg tcgtaataag ccgttgccgg aatagtcata tcagttttga tgagacctgg
    16981 gcgcacttca taaacgccaa tttgttcatt acaaaggcga gcagcaaata gacgagcagc
    17041 ggcagaaacg gcggttttgg caatggtgta ttcgccgcga ttcatcgcca gcatgatggc
    17101 gttgatggaa ctgacgaaaa taatcgagcg gtgcggcagt tccgcttccg gtttcggctg
    17161 tgccagtaag cgcttactga atgcctgagc aaggaagaac ggcgcgcggg tgttaatggc
    17221 aatgttttga tcgaagctgt ctggttcgag gtcgaggagg tcaccgcgtt ttttcactga
    17281 aatcccggcg ttattgagca ggcagtccag acgcccccac tggttttgcg ccgcgtcgag
    17341 catctcttca tgcaaggaga gatcgccgac atcggcaggg aagcaaatga cttccacgcc
    17401 ttctgccaaa cattcttgtt gtgtgatatg cagtttttct acgctgtcgg catcgggacg
    17461 atcgttaatc agcaggttaa acccgccacg agcaagctcc agcgcacagc cctttccaat
    17521 accacgagcg gctccggtaa tcacggcaac tggacgagtg gatttcatac aataaccctc
    17581 ttttgaatat tgttttcttt agtgatgatt agcttgcgac tggcaggtaa aaagatcaac
    17641 catctagttg gttgatctgc gagagtgatc acaggtaatt tgataactca atgaaattac
    17701 gacttttatc ctttttgctg tcgctgagat caaatccggt tatgatagcc atcacgaaga
    17761 ggaagtgggg aagtggaatg tatcttgata ccagtcacgc acaaaacctg aaggaaaaat
    17821 tgctgctatg tgcggttaat gaatttgccg aatatggtta tgaaggggcc agagtagata
    17881 atattgttaa ggcagcaggg tgtagtaaac agacggttta tcaccacttt ggtaataaag
    17941 aaaatttgtt tattgaagta ctggaatata cctggaacga tattcgcgaa aaagagagag
    18001 agcttgacat ttcagggctt tctccggtta ttgcgatcga gaaaataatt gattttactt
    18061 gggattatta catcaataat ccgtggtttt taaaaattgt ccatagtgaa aaccaaagta
    18121 agggtgtgca ttataaaaaa tcaaaacgcc tcgctgaaat taaccatgca catttgcagt
    18181 caatggcttc gttattagaa gaagggcagc gacagaaaac ctttaagaaa gatattgatc
    18241 ccttacaaat caatattaat atcgccgcac tgggtgcata ttatttaata aaccagtaca
    18301 cactggggat ggtttatcac attagcatgg tttcacctga ggctttggaa gccagacgca
    18361 gagtgatcaa agagaccatc ctgagctggc ttttggtgaa ccctgcccgt gggtaatcag
    18421 cgtgcgcgga tgaaattacc atcaggttgg cgggtgaaca gtacttgctg attatttccc
    18481 gtatcaatgg ttaagccggt aaccacccca ttcgcatttt gacggatttt aacaatctga
    18541 ccattttgca ggttactgag cggtttatcc gcgccctcga cctgggccat agcgtacaca
    18601 tctgtaggcg gcaaattatg gtcgcggaac agctgggcaa gggtcttgcc tggttctacg
    18661 cgatatgtcc gccactgttg atcgatacca ctttccggtt ggaaagattg tgtctctgca
    18721 acaggtggct cttcaggttg accttcctga ataggttccg gcgcgaccgg ggcgacctga
    18781 tccggatcgt tttgcggcgt cactaaatgt gcctgtagct gtgcttcacc tggggggggt
    18841 gattgcatat ctaactgcgc ttcgcgggtg acaacaggca tctggcgtgt ttcatctcca
    18901 ggcagaagaa taccgataat cagcactatt gccgcaataa tgattccccg gcgatgcatc
    18961 ggcggcaacg ggtccatgag gcgaaagtta tctggtgcgt gccagatttt caccagagtt
    19021 ggttttagtt caaagcgccc aggcatggtt ttcctccagc tccgcgtcgt gttcctcaat
    19081 catagcgcag taataagcta acgctatgat gtccgtggta ttaccgctgt ttttgtattc
    19141 atgcggatta tccctattgt ttctgtttcc ctgggatttg tcactcatcc cgcgacaaag
    19201 ttttaccaga agaagcgtgg ctgatatcct gcccgctact ttatacccta agaaaggaaa
    19261 tacgatgacc accccaactt ttgacaccat cgaagcgcaa gcaagctacg gcattggttt
    19321 gcaggtaggg caacaactga gtgaatctgg cctggaaggg ctgctgccag aagcactggt
    19381 tgcaggtatt gccgatgcgc tggaaggcaa acatccggct gttccggttg atgtggtgca
    19441 tcgcgcgctg cgtgaaatcc acgagcgcgc cgatgccgtt cgtcgtcagc gtttccaggc
    19501 gatggctgct gaaggtgtga aatacctgga agaaaacgcc aaaaaagaag gtgtgaatag
    19561 caccgaatct ggcctgcaat tccgcgtgat caaccagggt gaaggcgcaa ttccggcacg
    19621 taccgaccgc gttcgtgttc attacaccgg taaactgatc gacggcaccg tgtttgacag
    19681 ctccgttgct cgtggtgaac ccgctgaatt tccggttaat ggcgtgatcc ctggctggat
    19741 tgaagcactg actctgatgc cggtaggttc taaatgggaa ctgactatcc cgcaggaact
    19801 ggcatatggc gagcgcggcg caggcgcatc catccctccg ttcagcaccc tggtgtttga
    19861 agtcgaactg ctggaaatcc tctaagcagc gcattctgtt cccctcgaac cagaggggag
    19921 caggcattca gcaataaacc cttcagtttg ccaaacggcg ctattttgtg ttgcaaagac
    19981 cccgtaagcg tgtatttttg tgagctgttt cgcgttatca ccgtgatatg acactcactt
    20041 taaacataaa attaacatta gatctaaatc ttagtattca tcccgcgtat tgttacctaa
    20101 tatcgatgag tcccgataca gattcgtcgt atcatagact gactaaaggc cgtagagcct
    20161 gaacaacaca gacaggtaca ggaagaaaaa aacatggtag atcaggtaaa agtcgttgcc
    20221 gatgatcagg ctccggctga acagtcgcta cggcgcaatc tcacaaaccg acatattcag
    20281 cttattgcca ttggcggtgc cattggtacg gggttgttta tggggtctgg caaaacgatt
    20341 agccttgccg ggccgtcgat cattttcgtt tatatgatca ttggttttat gctctttttc
    20401 gtgatgcggg caatggggga attgctgctt tcgaatctgg aatacaaatc ttttagtgac
    20461 ttcgcttccg atttactcgg gccgtgggca ggatatttca ctggctggac ttactggttc
    20521 tgctgggttg taaccggtat ggcagacgtg gtggcgatca cggcttatgc tcagttctgg
    20581 ttccccgatc tctccgactg ggtcgcctcg ctggcggtga tagtgctgct gctgacgctc
    20641 aaccttgcca ccgtgaaaat gttcggtgag atggagttct ggtttgcgat gatcaaaatc
    20701 gtcgccatcg tgtcgctgat tgtcgtcggc ctggtcatgg tggcgatgca ctttcagtca
    20761 ccgaccggtg tggaagcgtc attcgcgcat ttgtggaatg acggcggctg gttcccgaaa
    20821 ggtttaagtg gcttctttgc cggattccag atagcggttt tcgctttcgt ggggattgag
    20881 ctggtaggta caacagctgc ggaaaccaaa gatccggaga aatcactgcc gcgtgcaatt
    20941 aactctattc cgatccgtat cattatgttc tacgtcttcg cgctgattgt aattatgtcc
    21001 gtgacgccgt ggagttcggt agtcccggag aaaagcccgt ttgttgaact gttcgtgttg
    21061 gtgggtttgc ctgcggctgc cagcgtgatc aactttgtgg tgctgacctc tgcggcgtct
    21121 tccgctaaca gcggcgtctt ctctaccagc cgtatgctgt ttggtctggc gcaggaaggt
    21181 gttgcgccga aagcgttcgc taaactttct aagcgcgcag tacccgcgaa agggctgacg
    21241 ttctcgtgta tctgtctgct cggcggcgtg gtgatgttgt atgtgaatcc tagtgtgatt
    21301 ggcgcgttca cgatgattac aaccgtttcc gcgattctgt ttatgttcgt ctggacaatt
    21361 atcctttgct cgtacctggt gtaccgcaaa cagcgtcctc atctgcatga gaagtcgatc
    21421 tacaagatgc cgctcggcaa gctgatgtgc tgggtatgta tggcgttctt tgtgttcgtg
    21481 gtcgtgttgc tgacactgga agatgacact cgccaggcgc tgctggttac cccgctgtgg
    21541 tttatcgcgc tggggttggg ctggctgttt attggtaaga agcgggctgc tgaactgcgg
    21601 aaataaccgc attatcatgc tggatggcgc aatgccatcc agcttttaga tcactcaccc
    21661 gccagcgcgc gtgggaacag tacattgttt tccaggctga tgtgatccat caggtcatca
    21721 atcagttcat taatgccgtt atacatcgct ttccaggtgg tgcaggcttc cggcggcggt
    21781 gtgacgttat tggtagtgtg tttgattact tccagcagtt cacccgcttc atcgtgctcg
    21841 ctttccatta cgctgattgg ccccattgcc tggctgccca tgccttgttt gatcatcggg
    21901 aagaggatct gctcttcttt catcatgtgg ctggaaagct cttcatgcag catagtcagg
    21961 tattttgtca gcccttttgg cacgctcggt ttgtcggcgt gaacgcgctc gactttagtc
    22021 gcttgcagga tcagctccgg caactgctcg cggtgacgat cgtggtagcg cacgatgata
    22081 tggtcaatga tttctgccag cggggcgcta cgccagtctt tctcaatcgg ttgttcagcg
    22141 agctttgcta gttcagcttc aatgacctca acatccagtt ctttacgtgc cgccgcgcgc
    22201 gccagcgtct gcttaccgcc acagcagtaa tccatatcat atttacgaaa cagagctgaa
    22261 gcgcgaggaa tagagagcgc cagttcacct aaaggttggt cgcgataagc catagctgat
    22321 acctcattct taataagata agatgtattt taaatgcatc tttaaggcaa aagctataac
    22381 ccttacgtag cagaggctta tttaactcac tgccagcgtg tcaactttct gagacagatt
    22441 cagttaaagg tttttctggt tttgctctga ccgcgatgac cacgccaatc actaacagcg
    22501 cgataccgct cagcgtcatt agcggcggca tttgctggcg gagtaaaaag gtgtacagca
    22561 aacctgccag cgtttcgaaa acaatcagcg gcccgagaat cactgtcggt aatcgctggc
    22621 tggcgacgtt ccagcagagt gcgccaaccc atgagcaaag cacggctatc gcaatcatca
    22681 gactaataaa caccagcgga cgggggccaa agggtaggga gaagtccggc gtttgcgtat
    22741 tcagccagta acaagcgacg agatagccga taagtgaaac cggcagtgtg accagcgcct
    22801 gcgctgttgc ccacatcatc ggatgtttgt cgggattttc ccgcagccag cgggcgttgc
    22861 gcagggcata ccacgcccag cagaccacgg aaactaacgc tagcacgatg ccagaggtat
    22921 aacgtgccca gtcaaaatcg gggagtccgt ggtttaactc agcaatattc acactcgcga
    22981 ggccgatgcc aatacaaatc agtgccgggg cgagttttcc ccacgcgagt ttgccgtcgc
    23041 gctggctata aagcagattg gcaaagacag gaatgaccac cggcagggtg ccgataatca
    23101 tcgtggaaac aggcgcgcca gtacgttgaa tggcactggc aaggcagaag taataaatga
    23161 ggttgcccat catagtgagc atcaaggcgg taagccagtc gcgacgcgcc aactgacgca
    23221 gacgcacgcg tcccagccag gcaatggg
//
LOCUS       DAFATA010000068        23237 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFATA010000068 DAFATA010000000
VERSION     DAFATA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.069"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..287)
                     /gene="ygiW"
                     /locus_tag="LGJ20_003154"
     CDS             complement(<1..287)
                     /gene="ygiW"
                     /locus_tag="LGJ20_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBK2387179.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNG"
     gene            439..1098
                     /gene="qseB"
                     /locus_tag="LGJ20_003155"
     CDS             439..1098
                     /gene="qseB"
                     /locus_tag="LGJ20_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBK2387180.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            1095..2444
                     /gene="qseC"
                     /locus_tag="LGJ20_003156"
     CDS             1095..2444
                     /gene="qseC"
                     /locus_tag="LGJ20_003156"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBK2387181.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(2526..2822)
                     /locus_tag="LGJ20_003157"
     CDS             complement(2526..2822)
                     /locus_tag="LGJ20_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBK2387182.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCG"
     gene            3141..3722
                     /gene="mdaB"
                     /locus_tag="LGJ20_003158"
     CDS             3141..3722
                     /gene="mdaB"
                     /locus_tag="LGJ20_003158"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBK2387183.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYVVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            3753..4067
                     /locus_tag="LGJ20_003159"
     CDS             3753..4067
                     /locus_tag="LGJ20_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK2387184.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(4111..5442)
                     /locus_tag="LGJ20_003160"
     CDS             complement(4111..5442)
                     /locus_tag="LGJ20_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387185.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDITNTRWGKYRPWLLFMSIPFGISIWLMFTTPDTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGTMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLSGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAYLDYQPGV
                     EQTPQALWGICLLMTIIPAILNLLTGLIMRFYLINNEYYEQIKARLQTAEE"
     gene            5729..6481
                     /locus_tag="LGJ20_003161"
     CDS             5729..6481
                     /locus_tag="LGJ20_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2387186.1"
                     /translation="MIRIYKASKGQLKHREIFDEIKKKIDNGEWKEGKAIPTERELAE
                     HFLSSRTTIRKAIESLKQRGYLHSVHGQGTFTLPARSRENHLLHSFTDDIKARGGVPA
                     QNILEIGYIPLSDIIRKNLELDIHVHTVFCIKRIRYMGSTPLGIQTSWLALNDGQEIT
                     LEELLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            6495..7571
                     /locus_tag="LGJ20_003162"
     CDS             6495..7571
                     /locus_tag="LGJ20_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBK2387187.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKTQWGG
                     FLNAFVSPPADTFAGSLNGVAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACRLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDVEAIASGLTLLAVQAQEK"
     gene            7571..8569
                     /locus_tag="LGJ20_003163"
     CDS             7571..8569
                     /locus_tag="LGJ20_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK2387188.1"
                     /translation="MNMPARAPVMPLAQTREEINATAAALRHLAAIWGECFSSVQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCSALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSLTVDALSHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYTLLRLAQNLCPGLTPLQIEEDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATAFPLEEFYHSLRLHQAGVGQHYFLLPATSREYPFYIATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            8574..9488
                     /locus_tag="LGJ20_003164"
     CDS             8574..9488
                     /locus_tag="LGJ20_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBK2387189.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYYYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYAAEPDFTLLHDIQDEYQLEAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPIIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLATPSEVNLFLSQHD"
     gene            9518..10641
                     /locus_tag="LGJ20_003165"
                     /pseudo
     CDS             9518..10641
                     /locus_tag="LGJ20_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
     gene            10776..11471
                     /locus_tag="LGJ20_003166"
     CDS             10776..11471
                     /locus_tag="LGJ20_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBK2387190.1"
                     /translation="MLSCLSPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSN
                     GIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQ
                     YWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQ
                     KTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMGSWIPYMSIADYKGVDKTSSN
                     RQIRWRWGLTYTF"
     gene            11485..12354
                     /locus_tag="LGJ20_003167"
     CDS             11485..12354
                     /locus_tag="LGJ20_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HBK2387191.1"
                     /translation="MNNKFKIILWMILSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(12399..14291)
                     /gene="parE"
                     /locus_tag="LGJ20_003168"
     CDS             complement(12399..14291)
                     /gene="parE"
                     /locus_tag="LGJ20_003168"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK2387192.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(14320..14901)
                     /gene="yqiA"
                     /locus_tag="LGJ20_003169"
     CDS             complement(14320..14901)
                     /gene="yqiA"
                     /locus_tag="LGJ20_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBK2387193.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14901..15728)
                     /gene="cpdA"
                     /locus_tag="LGJ20_003170"
     CDS             complement(14901..15728)
                     /gene="cpdA"
                     /locus_tag="LGJ20_003170"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBK2387194.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15753..16175)
                     /locus_tag="LGJ20_003171"
     CDS             complement(15753..16175)
                     /locus_tag="LGJ20_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBK2387195.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(16176..16805)
                     /gene="nudF"
                     /locus_tag="LGJ20_003172"
     CDS             complement(16176..16805)
                     /gene="nudF"
                     /locus_tag="LGJ20_003172"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBK2387196.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            17010..18491
                     /gene="tolC"
                     /locus_tag="LGJ20_003173"
     CDS             17010..18491
                     /gene="tolC"
                     /locus_tag="LGJ20_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBK2387197.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18639..19310
                     /locus_tag="LGJ20_003174"
     CDS             18639..19310
                     /locus_tag="LGJ20_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBK2387198.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            19316..20476
                     /locus_tag="LGJ20_003175"
     CDS             19316..20476
                     /locus_tag="LGJ20_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBK2387199.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(20514..21302)
                     /gene="ygiD"
                     /locus_tag="LGJ20_003176"
     CDS             complement(20514..21302)
                     /gene="ygiD"
                     /locus_tag="LGJ20_003176"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBK2387200.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            21445..22218
                     /gene="zupT"
                     /locus_tag="LGJ20_003177"
     CDS             21445..22218
                     /gene="zupT"
                     /locus_tag="LGJ20_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBK2387201.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(22276..22446)
                     /locus_tag="LGJ20_003178"
     CDS             complement(22276..22446)
                     /locus_tag="LGJ20_003178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387202.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22707..>23237)
                     /gene="ribB"
                     /locus_tag="LGJ20_003179"
     CDS             complement(22707..>23237)
                     /gene="ribB"
                     /locus_tag="LGJ20_003179"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBK2387203.1"
                     /translation="DMIFPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVE
                     NNTSAYGTGFTVTIEAAEGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQ
                     AGGVLTRGGHTEATIDLMTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVT
                     IEDLVAYRQAHERKAS"
BASE COUNT         6332 a         5653 c         5475 g         5777 t
ORIGIN      
        1 ccgttccagc gtttgtggtc gatatcaaca ttgatagtac cgctggcatc tttaaacacg
       61 tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca
      121 cgcagggatt ttgcgctttc tacggtcgtt acgctgccgt tcggcccctg gaatcctccg
      181 gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc catcaccggt
      241 gcgctgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt tattactccc
      301 tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt aagggaaaaa
      361 aataaaattt agtgctgtac agagcgcgtt acaacacggt ttactggcag caaatacggt
      421 tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct gattggcgac
      481 ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg actggtttac acaaggtcgt
      541 cagggaaaag aggcgcttta tagcgcgcct tatgatgcgg tgatcctgga tttaacctta
      601 ccaggcatgg atggtcgcga tattttgcgc gaatggcgag aaaaaggtca gcgtgagccg
      661 gtactgatcc tgaccgcgcg cgatgcgctg gaggaacgtg tagaagggct gcgtctggga
      721 gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag gctggaagct
      781 ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa cgtcatgctc
      841 gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa accaaaagaa
      901 tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg caaactgatt
      961 gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga agtgcatgtg
     1021 catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg tattggctac
     1081 acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa
     1141 tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa
     1201 caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc aagcggttaa
     1261 gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg ccaaataaat
     1321 taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc cacgacggca
     1381 gaatggtcct taacgatggc gataacggag aagatattcc ttatagctat caacgggaag
     1441 gttttgctga cgggcaactg gtgggcgata aagatcaatg gcgttttgtc tggatgacct
     1501 cacctgatgg caaatatcgc atcgttgttg gtcaggaatg ggaataccgt gaagacatgg
     1561 cgctggcgat tgttgccggg caattgatcc cgtggctggt cgcactgccg attatgttaa
     1621 tcatcatgat ggtactattg ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcgc
     1681 tgcgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta cccagcgaag
     1741 tgcgtccgtt ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg atgatggttc
     1801 gtgaacgacg ctttacctcc gacgcagctc acgaactacg tagcccgtta acggcgctga
     1861 aagtgcaaac cgaagttgcg cagctttctg acgatgatcc gcaagcgcgg aaaaaagcgc
     1921 tgcttcaatt acattacggg atcgatcgcg ctactcgtct ggttgatcaa ctgctcactc
     1981 tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg cttgaagatc
     2041 tcctgcaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa attgacgtgc
     2101 gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg ctaagtttgt
     2161 tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc gtgatagacg
     2221 tcacgctgaa tgctgataat ttcatcgtga gggataacgg ccccggtgtg acgccagagg
     2281 ctctggcgcg aattggcgaa cgcttctatc gcccccccgg acaaaccgct accggcagtg
     2341 gacttgggct atcgattgtc cagcgaatcg ccaaattgca tggcatgaat gttgagtttg
     2401 ggaatgcgga acaaggtgga tttgaggcga aggtaagctg gtaacttctc tgtctatgag
     2461 agccgttaaa acgactctca tagattttac taatagcaaa atataaaccg tccccaaaaa
     2521 agccactaac cacaaaccag acaaataccg agcgaaaata catataccca aaactcgaac
     2581 atttcccgca taaagagttt tcttaagata agaataataa gtggcgtaag aagaaaaaat
     2641 gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat actgcaaata
     2701 ttgcctcccg actcaagcat ccagtcatac ttattgaaag caaaaaaagc atctattaac
     2761 caataagtaa gcattatgta taacagagct tcaaatattg tttgaatttt atgctttaac
     2821 atatatcagt acgctcataa aaatccattt ataattatca acatgacacg ttgatatttt
     2881 catcttttac ttattcaaag ccatattttc aatcccaaaa aaaaccgaac caaacgtgta
     2941 aattattccg gtgcgagtaa gaattatgag gaatggctat cagtattgtc attttcagaa
     3001 aatatttatc ctgcatcggt gagtcagagc tagatcagac ttttgctaaa ttcgcaaaag
     3061 actttgcaca ttttgctaat ttcaccgtac cgctctgtga cgtactatag tcgacaaacg
     3121 tctcaccttg aggttaaaaa atgagcaaca tcctgattat caacggcgcg aaaaaattcg
     3181 cccactccaa tggtcaactg aacgacacac tgaccgaagt cgcggatggc acactgcgcg
     3241 acctcgggca tgatgtccgc atcgttcgcg ccgacagcga ctacgttgtc aaagcggaag
     3301 tacaaaactt tctctgggct gatgtggtga tctggcagat gccaggctgg tggatgggcg
     3361 cgccgtggac agtgaaaaaa tatattgatg atgtattcac cgaaggtcat ggaacgctgt
     3421 atgccagcga tggtcgtacc cgcaaagatc cgtcgaaaaa atacggttcc ggcggcctgg
     3481 tacagggcaa aaaatatatg ctttctctaa cctggaacgc accaatggaa gccttcaccg
     3541 aaaaagatca gttcttccac ggcgttggcg ttgacggtgt gtatctgccg ttccataaag
     3601 caaaccaatt cctcggtatg gaaccgctgc cgacatttat cgctaatgac gtgataaaaa
     3661 tgcctgatgt tccccgctat actgaagaat atcgcaagca tcttgtggaa atttttggtt
     3721 aactagagct caggctttag aaggagttaa ccatgcttac cgtaatcgca gaaatccgta
     3781 ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca gtttgctaaa atcgttccaa
     3841 ccgtactgaa agaagaaggt tgccacggct atgcgccaat ggtggattgc gctgctggcg
     3901 tgagtttcca gtctatggca ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg
     3961 cgcatcttga agcgcatctg caaaccccgc acatgaaggc gtacagcgaa gccgtaaaag
     4021 gcgacgtgct ggagatgaat attcgtattc tgcagccagg gatttaatat ttctgaaaag
     4081 aggccggtgc tgcttgccgg ccatttattt tcactcttcc gccgtttgta accgtgcttt
     4141 tatctgttcg taatattcat tattaatcag ataaaatctc atgatgagtc cggtaagcag
     4201 gttaagtatc gcgggaatga tcgtcattaa caggcagatc ccccacagcg cctgtggtgt
     4261 ttgttctacg cctggttgat aatccagata ggctaaagcg attccgataa agccccctgc
     4321 cagccccata ccgaactttt ggaaaaagag aatcccgcca aatgccaggc cagaaacacg
     4381 ttgccccgtt ttcatttcac cgtaatccac ggactcagca atggatgccc aataaatggg
     4441 aagctggata tccgtcagga aagtcaccac aatataaaat gtaaaagcta gcactataga
     4501 atttggttgc acgaaataca tcgcaccacc caccacgaac gccagtaatt gggtataacg
     4561 aaatattttt attttgtcat agagacgagt aagccaggtg caagcgatca tagccagaac
     4621 tgaggcgatc acgcctgaag ttaaaaacgg tgaaattaaa gcatccccac cactcaggta
     4681 atattttgca taatatgcag cgacagagtt acgaataatc ccgccaaaca taatgatcgc
     4741 aattacagcg cccaaaatga gccattgatc atttttgatc agtaaccgaa actgcgcggc
     4801 cataccgaga ctgggttttg gatggtagat gcgctctctc acgctaaaaa aacaatacag
     4861 gcataaacac gttgccagaa tacccattgt ccccattgct atttgataac ccagttgtac
     4921 gttattcttc ccaagataca ttgcagataa aggaacaata attgttacgg cgaacatggc
     4981 aatttttgtc ataacaaaac gataaccatt ggcgctcaat ctctcttgtg gatcatccgt
     5041 gataacgctg attaatgaaa cataaggaat agcgattaac gtataggtca aggtaagtaa
     5101 cacataggta gcccaggccc ataaaagctt aactgaataa tcggtatcag gtgtggtgaa
     5161 catcaaccaa atgcttatcc caaacggtat tgacataaaa agcaaccatg gccgatattt
     5221 tccccaacgt gtattcgtta tgtctgtcat tgttcccaca actggatcaa tcacagcatc
     5281 aagaatacgt acagaaaata acagaatacc taagtcgacg ggtttcagac caaatacatc
     5341 cgtgtaaaaa taagtgatga tcatggctaa agacatcatt actatcgcaa tggccatatc
     5401 accggcacca aaacctatct tttcatttat tgtcagtttc atacacagca ctcattaatc
     5461 acatgaaatt atattaaaaa caaatgtatc ggaactcatt cagcacataa ctttacgatt
     5521 gagaataaat gcattggtag ataccacaac aaaccactga gttaatattg agcagcatca
     5581 cataaaaacc aatttcactt aaccgcatga tatttattaa taaatgtggt ttttcctgtt
     5641 ttatgtcaca gataacaaat aagcatactg cgcaataaaa ataaaagaat gtaaagttgg
     5701 tatcaaccac ttgaaatgag gctttgtcat gataagaatt tataaagcca gtaaaggtca
     5761 gctaaagcat cgtgaaattt ttgatgaaat aaagaaaaaa attgataacg gtgaatggaa
     5821 agaaggcaaa gctataccaa cagaaagaga actggctgaa cattttttaa gcagcagaac
     5881 aacaattcgg aaagcaatag aaagtttgaa acaacgcgga tacctgcata gtgtccacgg
     5941 gcaaggaaca tttacattac cggctcgcag tcgggaaaat catttattac atagttttac
     6001 tgatgacatt aaggctcgtg gtggcgtacc ggcacagaat attcttgaaa taggttatat
     6061 tcctctcagt gatattatca gaaaaaatct cgaacttgat atccatgtcc ataccgtttt
     6121 ctgtataaaa cgcattcgct atatgggaag tacgccgctg ggtattcaga catcatggct
     6181 tgcgttaaat gacggacaag aaataactct ggaagagctg ctggcgacgg gttcgctcta
     6241 tattttactg gaagaaaaac tcgctattaa acctttagaa gcaacggaga ttatctctgc
     6301 ccggcttccc tcgccccttg agcgtcaatt actggaactg actgccgatg acgtcgtatt
     6361 aagttgtacc cgcgtcacgt tatccgtgga acgtaaacca atggagtatg tagagatggt
     6421 ttatcccgca agtcggtact cctacaaagc acgaattaca aaagacagtt ttagcgtgta
     6481 acagagagaa tattgtgaac aaaatagaac aagccgtaat ttataacaaa atattaggtt
     6541 ctttagcctg cgcaggtatg ggcgatgcat taggcgcagc gactgaactc tacagcattg
     6601 atgaaataaa aactcaatgg ggaggttttt taaatgcctt tgtttctcct cctgccgata
     6661 cctttgctgg ctcattaaat ggtgttgcag gacttatcac cgacgattcc agccaaatgt
     6721 atgtgttttc agaagggtta attgaggctg gatttgataa cttcaccaat aatgactggt
     6781 tagcttgtct tctgcgttgg gccgatatgc agccctacgc aaattataaa ggccccacaa
     6841 cagaacaaat cgtcaaggca ctaaaagaag gtagaccaac aaacaccatt gggcgtattg
     6901 gaacatcttc tcgtcaggcc cccaatgtcg gcaccacgaa tggcgcagga atgcgtgtag
     6961 ccccggcagg attgatctgg cctggcaaga aagaaaaagc gtgtcgcctg gcgttaataa
     7021 cttgcctacc ttcacatgac accaatattg cgattgccag cgcttgcgcc atcgctgcag
     7081 cgacatcgca ggctatgtta ccagaagcct cgctcacatc attactggat gccgcaatct
     7141 ggggagcgaa ttacggcgaa cgcctggcca aacaatatgc acgatgtgta gcagggccat
     7201 ctatcgccat gcgcatccaa cttgcagcgg acatcgccag acgtgccaat gatctggagt
     7261 cttgtctgcg agaaatggaa ggtttggttg gtaactctgt tgccgcccat gaatccattc
     7321 ctgcggcaat cggtctattg ctgtactgca aaggagaacc ctgggaaacc atccatgcct
     7381 gcgccaatat tggcaatgac accgatagca tcgctacgat ggcaggcgca attgcagggg
     7441 catggcgcgg ttttgatgcg ctccctgaag ataaatatgc ctttttccgc gccgtaaata
     7501 ataaagattt cgatgttgaa gcgatcgcat cgggtctgac attgcttgca gtacaggcac
     7561 aggagaaata atgaacatgc cagcccgtgc tcctgttatg ccattagcgc aaactcgtga
     7621 ggaaataaat gcaacggcgg cagcgctacg ccatttggca gctatctggg gtgaatgttt
     7681 ttcatctgtt cagctcgata aacgtgctgt tgaacgcgtc tgtatagtgg gatcgggcga
     7741 ctcctggacg gtggcactat gtgccgccgc ctggctgggg aaatacacac atctgttttg
     7801 ttccgcattg caaacgtggg attttcttca gactgattta acccgttatc agaaagaaac
     7861 gttgatcatt attctcagcg caagtggcag accatcactg accgttgacg cccttagcca
     7921 cgcagtatgc tcaaacgcgc aagtgctggg tgtgacgaat tgccctggaa caccattttg
     7981 cgcaataaca gaaaacatgc tctatacatg ggcgaataag cagggtattc caacgcaaag
     8041 tagtagcgtc acactttata ccctcctgcg cttagcgcaa aacttatgtc caggcttaac
     8101 accactgcaa attgaagagg atttagaagg gaaatttagc cagattaatc agcactggca
     8161 gcaaaagaag cgccattttt accaacaaaa agagataacg tttctgggga gtggcttaag
     8221 ttggggacta gcaatatcgg ggagcaattt actttcttgc ggcccacaga ttcgtgcgac
     8281 agcctttcca ctggaagaat tttatcattc attacgctta catcaggctg gtgtcggtca
     8341 gcattatttt ttactgccag ctacatcccg cgagtaccca ttttacattg caacccaaaa
     8401 agcaattgtt gaacagggag cgacagcaga attaatcagt tttatacctg atgccagtga
     8461 gtccaacaat ttatttctgg tcatgcagtg gttatatgaa atgtgctggc atttaagttg
     8521 tgattatgtc gacgcaggcg ggcaacgcgt tagccatagg gagaaataag actatgaaga
     8581 aatttgatgt cgccgcttta ggcagtggaa atatagatat ttttttatct atcccgtcac
     8641 tccccactcg tggcggtaaa gtggtcggga cacgtctggg tgaacaagct ggcggcacag
     8701 tcgccaacag tgcctgtgcg atgggacaac tgggtctcaa tgtggtatct gtttcatgtg
     8761 tgggcaatga tcattcagcc tcgatcattc ttgatggatt taaaaaatat catgtaaatt
     8821 gtgatttcgt tcaggtaatt ccggaattga ttgccaatac cgcgattatc tttattgatg
     8881 aattaggtga aaaaacgttg gtttactcgc ctggtagtga tcatgagtgg gataaagaaa
     8941 aagccctgca ggcaattgcg caaagccgtt attattatac gatgccagca gatatcgaga
     9001 aatttcgtat gctggccgaa tattcccgta gccagatgac aaaagttgtc gttgatattg
     9061 agcctcatat cattgcaaca cctgaacacc ttgccagaat tttacacctt gctgatattg
     9121 cgatatttaa ttatgacggt tttattcgtg gctatgcagc agagccagat tttacacttc
     9181 tgcatgatat acaagacgag tatcaacttg aggctgtcgt ggttacgctg gatgttaggg
     9241 gagtaattgc agtcaaaggc aatgagcaag taaaaatagg tagctataca attcccatta
     9301 ttgataccac tggcgctgga gatactttta atggtgcatt tgtttatagc ttaataaaaa
     9361 acatgccctt aattgaagca ctaaaatttg cttctgccac tgcagcaata aatattactg
     9421 cattaggtgc cagaggtcat ctggcgacac catcggaagt taatcttttt ctttcacaac
     9481 acgactaaaa tcagatacat acacaaaggt aaatatcatg aaaataagac atgccgctat
     9541 ttttctggca ttaccgctgt tatgcacaac agcgcaggca aacagttttc ttcattatgc
     9601 tggtgagttc aacttccgga cgggagaaaa acagaacaat gttacaatcg cggggctatc
     9661 agccatcaca tttgatgcaa aaaaaatatt ttctatgcta ttaacgatag cagaaacaat
     9721 aataaagaag gagatgccag tctttataca ctaaaaatac aggtatcatc caggggcatt
     9781 gagcaagtga attttctcaa ccagcgtcca ctgcttgatg caaaacagca gcctttcgtg
     9841 accaatacgg tggatgccga aggtctggcc ctgacacaca atggtaagtc attactctgg
     9901 agttcggagt taggtgctcc actgcgtctg agcactctcg atggcgtaat ggagaaagat
     9961 tttacgtctt tatttcctgc tcgatttaat attagttctg acaaagagag cagcaatggt
    10021 attcgcagcg gcaacgcctg ggagggcctg acagttacac cagatggaaa atcacttttt
    10081 atcgccgttg aaagcagcct taaacaggat gggcctattg caagtccaat aaattcaggt
    10141 acatcacgct tactgcaatt ttctatagat gcagacggtc gaccttcaaa gcaattgcat
    10201 gaatatctct atattactga tcctgttccg caagtgagta agttcggtat taatgataat
    10261 ggcgtatccg aggtcctggc gctgaatgat caccaattgt tggtcatcga acgttccggc
    10321 agaaacgtca gtgctgggtt caatgactgg gattactctg tcagagttta tatggtcgat
    10381 ttaactgctg ccagcgatat caaggatatt gacagtctgc aagattggtc caataaatcc
    10441 acgttacaac ccgtcagtaa aaaattatta attgattttg ctgattacac ttcctctgcg
    10501 gattgtattg aaggggtaac cttcggtcct ctgattgatg ggcatacatc gttaatattt
    10561 gtatctgata ataatttcca gccccatcaa cagacgaaat tttatctatt cattgataag
    10621 gaaaacaaac ttaaaattta aagtcaaact atagataaaa aaacattcta atacctggaa
    10681 tcatcagatg gtgtattcga tcaccaaaaa tatattttat tctgaaaggg aatattatga
    10741 attcttatct taaaatatca cttgcaggaa taacaatgtt atcctgcctg tcacctacac
    10801 tagtctgtgc agaacagccg catggattta ttgattatcg tcatgagtat cttgatgata
    10861 ctcgcaccca cgcggaccgc gttgaatttg gtacattttt tagtaatggt atcggtttga
    10921 tgggagaatt acgctataac accgatgaag gtgataaaga taagtgggac ccctcacaat
    10981 ttggcaataa cggaacagga ttatctgttg tttaccgctt taagccgctg gatgacaaaa
    11041 aattctggtt agaacccatg ttctggcttg ataccacgca atattggtca acttacgaat
    11101 atggtctttc tgcgggttat gatttcagca aagaatggaa agtttcgggt cgttttcgtt
    11161 atgacatgga taaagctacc gataaatcca aaggttatgg caacgatgat cgtaataacc
    11221 gccgttacga tgtgtggatc gactatcgac cacaaaaaac caacttccaa tatcagttaa
    11281 acctggtcta ttacaataat ggttatttaa cctggaatga tggccataaa aactataccg
    11341 catcatttaa agtagggtac aaaatgggat cgtggatacc ctatatgagt attgcggatt
    11401 ataaaggtgt ggataaaaca tcaagtaatc gccaaatccg ctggcgctgg ggtctgactt
    11461 atactttcta atctggaata aaaaatgaat aataaattta aaatcattct ctggatgata
    11521 ctctctgctc tgccagcttt atcttacgca caggtaaaaa atatagttac tggcgaatat
    11581 agtgcggtca gtaaaattat caccgaaaag gaaactctct atccatccga taagatatta
    11641 ttggtttttg acattgataa tacgttatta acgagtggca cacgcattgg cggtgatatc
    11701 tggtatcaat ggcaaaccga taaattgccc ttaaagccgg atgactcgca aaaagttccc
    11761 tgcctgtatg aaaatacaat cagtatgctt tacgaactta gccctatgca gcttaccgag
    11821 ccacagttac cgacaatgtt aaaacagtgg caacaaaagc ataccgcttt tgctctgaca
    11881 tcccgtgcac cggatactct ctttccgaca ttgcgggaat taaagcgaaa cggcattgat
    11941 ttttcgtctt ccgcactcag gaagaaagag gatactttgc caccctttga aaaagggaaa
    12001 ctcaaacggt catggctgta cagccagggg gtattttttt cttccgggca agataaaggc
    12061 gtaattctcg attatatgct ggataaaatg gggaacaaat atgacgcgat tgtttttatc
    12121 gatgacgggc aagcaaacat tcatgcgatg acgaagatgt tctctaagga taaatggtcc
    12181 tctacggatt ttaccattat tcactatacc agagtggagg atgagcttat taaacaacaa
    12241 ggtgcagtaa taacaactgc acaagcagaa aaaatggcta cggactggaa aactctggct
    12301 ggttcgctgg caacactatt tccagatcgt aacaaactgt gcccaataaa ataatcagcg
    12361 acaacccggc aaaacacatg tttgccgggt aaagattatt aaacctcaat ctccgccatg
    12421 tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata
    12481 tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg
    12541 cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct
    12601 ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct
    12661 ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt
    12721 ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa
    12781 gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt
    12841 ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa
    12901 atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg
    12961 atcttacctt tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg cttggcagat
    13021 ccgcctgcgg agtcaccttc cacaaggaac agttcggtac ggttaagatc ctgcgcggta
    13081 cagtcagcca gtttgccagg cagtgctggg ccgctggtca gctttttgcg caccactttt
    13141 ttagccgcac gcatacggcg ctgggcgctg gaaatcgcca tctccgccag caactccgcc
    13201 gcctgaacgt tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacaaat
    13261 gccgcgcact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc
    13321 atttttactg acagcacata ggcgcagcga tcccagatat cttccgccga caactttaca
    13381 ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga
    13441 cgcagaccgt taacgtgcgt accgccctgc atcgttggga taaggttgac gtagctttcg
    13501 gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg
    13561 ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc
    13621 gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca
    13681 tctttaaagg taatctcaac gccggggcac agtactgctt tggccttcag cacatgcgtc
    13741 aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg
    13801 ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg
    13861 ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc
    13921 ttcgacaggg cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa
    13981 tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt
    14041 acaccctctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac
    14101 gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg
    14161 acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc
    14221 cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa
    14281 gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag
    14341 aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc
    14401 tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc
    14461 atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat
    14521 gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt
    14581 ctcgttctga ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac
    14641 cactgcgggc agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga
    14701 accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca gcagctctgc
    14761 cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc
    14821 cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg
    14881 taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg
    14941 tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc
    15001 cagccgggcg cgatggtgtc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag
    15061 gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata
    15121 tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc
    15181 gcgttacgca ggctgtgttg atcaagccaa ctacaacccg caggtagcgg atgatgatgc
    15241 agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc
    15301 tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt
    15361 tgccattgct caccaataaa cacgcgcttc gccggggaga tacccgcatc ctgtaacgcg
    15421 ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa
    15481 cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca
    15541 cctgtcgcga caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc
    15601 tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga
    15661 gtatcggtaa tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg
    15721 ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca
    15781 tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg
    15841 cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg
    15901 cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg
    15961 ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc
    16021 gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg
    16081 ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc
    16141 atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct
    16201 tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc
    16261 tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt
    16321 tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt
    16381 gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt
    16441 ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca
    16501 ccctcttcta tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat
    16561 gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc
    16621 aagactgcgg cgtgaccgcg ctcaaaaatt tccctccgta cctcatgact catttgcccg
    16681 ttgaataaac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt
    16741 tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta
    16801 agcattgtta atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac
    16861 tgccgtttga gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc
    16921 caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc
    16981 taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc
    17041 tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc
    17101 aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg
    17161 aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca
    17221 cctatagcaa cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc
    17281 tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa
    17341 aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca
    17401 ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac
    17461 aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg
    17521 tagcgataac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag
    17581 tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact
    17641 actatccaga actggcagcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg
    17701 ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga
    17761 gccaggacct ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg
    17821 atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa actcgtggtg
    17881 ccgctggtac ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct
    17941 cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact
    18001 ttgttggtgc cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt
    18061 cctccttcaa caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag
    18121 tttccgctca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca
    18181 ttgttgatgt gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg
    18241 cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt acgttgaacg
    18301 agcaggatct gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa
    18361 acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc
    18421 cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt
    18481 tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga
    18541 tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta
    18601 ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac
    18661 gccacgcatc gttccgcaaa aactggagcg cgcgccatct gacaccagtc gctctcgcgg
    18721 ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc
    18781 aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt
    18841 acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact
    18901 gtgttgctga atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac
    18961 cagaaaacca ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg
    19021 gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct
    19081 cctcgaaaaa cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact
    19141 atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac
    19201 cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca
    19261 ctatgcagcg tagcgcaact ggcacctctt ctcgttcaat gggtggctga taccgatgga
    19321 aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa
    19381 ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct
    19441 cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt
    19501 ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacacac
    19561 ctggagtttt gtgcgccagt catggctgac gcaccagcca tcgctttatt cgcgtcttga
    19621 tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac
    19681 gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg
    19741 taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt
    19801 cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac
    19861 ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaacggaag ctgaaattgc
    19921 tactgagttc ctctacatcg atgatatcgg gttaggtgaa aaaggtcagt tcacggattt
    19981 acaggatcag gtaatttcca acctgttcaa gctgtatccg tgggaattta tgttgcgtga
    20041 gatgttttcc accaagctgg aggatgcagg cgtacgctgg ctggaaccag cgtggaagag
    20101 cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa
    20161 cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa
    20221 accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gaaaacggca aaaccattga
    20281 agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc
    20341 gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc aacccgccgg
    20401 aattggcatt cgtgaagacc gtgcattgat cacccaggat atgtctcggt tttatccaca
    20461 tatttttgtt gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct
    20521 atctgcaccg acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt
    20581 ggctcctgcc catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt
    20641 gggttcgata acgcgccacc ttcatggtcg aggtaattca ccagaggatg ctgctctacc
    20701 gccccttgcc acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat
    20761 ggtgaactat caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg
    20821 accaacatta ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag
    20881 gcggcaggtt tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac
    20941 atcttaatca gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc
    21001 accgggaccg gtgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca
    21061 ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc
    21121 gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc
    21181 gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct
    21241 tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac
    21301 atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg
    21361 gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag
    21421 gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg
    21481 gcagccacgt ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta
    21541 ctggcgtttt cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg
    21601 cttcctgccg cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc
    21661 ttcggtctgc ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat
    21721 ttaatgcaaa aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc
    21781 actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc
    21841 agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tattcctgaa
    21901 ggtctggcgg ttgcaggccc ggtttatgca gcaacgggtt ctaaacgcac ggcaattctg
    21961 tgggcgggga tttctggatt ggcagaaatt cttggtggtg tgctggcgtg gttaatcctc
    22021 ggtagcatga tttcccccgt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg
    22081 gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac
    22141 cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa
    22201 acggcgggaa ttggttaaaa gcaacatcag aagttggcca gcgcatggac aacttctgct
    22261 agcttcacta aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta
    22321 tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt
    22381 caaatgcagg aaataaccca ccacaacaag agcaatcaat acaatccagt accaggcgat
    22441 aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat
    22501 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
    22561 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
    22621 aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga
    22681 ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
    22741 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
    22801 tctggtgcac gcgccatcgt gccatcgtca ttagtcaact cacacagcac accagccggt
    22861 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
    22921 acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt
    22981 tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaattc gatcagcggc agaaacaccg
    23041 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
    23101 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
    23161 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
    23221 gccgggaaga tcatatc
//
LOCUS       DAFATA010000069        23128 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFATA010000069 DAFATA010000000
VERSION     DAFATA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.070"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..387
                     /gene="fxsA"
                     /locus_tag="LGJ20_003180"
     CDS             <1..387
                     /gene="fxsA"
                     /locus_tag="LGJ20_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBK2387204.1"
                     /translation="LTLVLVIFTSVIGMSLVRNQGFKNFVLMQQKMAAGENPAAEMIK
                     SVSLIIAGLLLLLPGFFTDFLGLLLLLPPVQKHLTVKLMPHLRFSRMPGGGFSAGTGG
                     GNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(403..1659)
                     /gene="yjeH"
                     /locus_tag="LGJ20_003181"
     CDS             complement(403..1659)
                     /gene="yjeH"
                     /locus_tag="LGJ20_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBK2387205.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            1935..2228
                     /locus_tag="LGJ20_003182"
     CDS             1935..2228
                     /locus_tag="LGJ20_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK2387206.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2272..3918
                     /gene="groL"
                     /locus_tag="LGJ20_003183"
     CDS             2272..3918
                     /gene="groL"
                     /locus_tag="LGJ20_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK2387207.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            4056..4409
                     /locus_tag="LGJ20_003184"
     CDS             4056..4409
                     /locus_tag="LGJ20_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBK2387208.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PADSQIIGQVYKCPN"
     gene            complement(4612..5481)
                     /locus_tag="LGJ20_003185"
     CDS             complement(4612..5481)
                     /locus_tag="LGJ20_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBK2387209.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5871..6899)
                     /gene="epmB"
                     /locus_tag="LGJ20_003186"
     CDS             complement(5871..6899)
                     /gene="epmB"
                     /locus_tag="LGJ20_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBK2387210.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            6941..7507
                     /gene="efp"
                     /locus_tag="LGJ20_003187"
     CDS             6941..7507
                     /gene="efp"
                     /locus_tag="LGJ20_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK2387211.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            7559..7684
                     /gene="ecnA"
                     /locus_tag="LGJ20_003188"
     CDS             7559..7684
                     /gene="ecnA"
                     /locus_tag="LGJ20_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBK2387212.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            7795..7941
                     /gene="ecnB"
                     /locus_tag="LGJ20_003189"
     CDS             7795..7941
                     /gene="ecnB"
                     /locus_tag="LGJ20_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBK2387213.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            8116..8433
                     /gene="sugE"
                     /locus_tag="LGJ20_003190"
     CDS             8116..8433
                     /gene="sugE"
                     /locus_tag="LGJ20_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBK2387214.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(8430..8963)
                     /gene="blc"
                     /locus_tag="LGJ20_003191"
     CDS             complement(8430..8963)
                     /gene="blc"
                     /locus_tag="LGJ20_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBK2387215.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(9052..10185)
                     /locus_tag="LGJ20_003192"
     CDS             complement(9052..10185)
                     /locus_tag="LGJ20_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBK2387216.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(10248..10607)
                     /gene="frdD"
                     /locus_tag="LGJ20_003193"
     CDS             complement(10248..10607)
                     /gene="frdD"
                     /locus_tag="LGJ20_003193"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBK2387217.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(10618..11013)
                     /gene="frdC"
                     /locus_tag="LGJ20_003194"
     CDS             complement(10618..11013)
                     /gene="frdC"
                     /locus_tag="LGJ20_003194"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBK2387218.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(11024..11758)
                     /gene="frdB"
                     /locus_tag="LGJ20_003195"
     CDS             complement(11024..11758)
                     /gene="frdB"
                     /locus_tag="LGJ20_003195"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBK2387219.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(11751..13559)
                     /gene="frdA"
                     /locus_tag="LGJ20_003196"
     CDS             complement(11751..13559)
                     /gene="frdA"
                     /locus_tag="LGJ20_003196"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBK2387220.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            13884..14861
                     /gene="epmA"
                     /locus_tag="LGJ20_003197"
     CDS             13884..14861
                     /gene="epmA"
                     /locus_tag="LGJ20_003197"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBK2387221.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            15080..16582
                     /gene="yjeM"
                     /locus_tag="LGJ20_003198"
     CDS             15080..16582
                     /gene="yjeM"
                     /locus_tag="LGJ20_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBK2387222.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(16733..20056)
                     /gene="mscM"
                     /locus_tag="LGJ20_003199"
     CDS             complement(16733..20056)
                     /gene="mscM"
                     /locus_tag="LGJ20_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBK2387223.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPQLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(20078..21046)
                     /gene="psd"
                     /locus_tag="LGJ20_003200"
     CDS             complement(20078..21046)
                     /gene="psd"
                     /locus_tag="LGJ20_003200"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBK2387224.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(21143..22195)
                     /gene="rsgA"
                     /locus_tag="LGJ20_003201"
     CDS             complement(21143..22195)
                     /gene="rsgA"
                     /locus_tag="LGJ20_003201"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBK2387225.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            22290..22835
                     /gene="orn"
                     /locus_tag="LGJ20_003202"
     CDS             22290..22835
                     /gene="orn"
                     /locus_tag="LGJ20_003202"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBK2387226.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            23046..23121
                     /locus_tag="LGJ20_003203"
     tRNA            23046..23121
                     /locus_tag="LGJ20_003203"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23079..23081,aa:Gly,seq:gcc)
BASE COUNT         5502 a         5873 c         6078 g         5675 t
ORIGIN      
        1 ctgaccctcg tgctggttat attcacgtca gttatcggga tgtcattggt acgtaaccag
       61 ggctttaaga atttcgtgct gatgcagcaa aaaatggcgg cgggtgaaaa cccagcggcg
      121 gagatgatta aaagtgtttc gctgatcatt gccggtttgc tgcttttatt accgggcttt
      181 ttcaccgact tcctcggtct tctgctttta ttaccgccgg tgcaaaagca tctgacagtg
      241 aagttgatgc cgcatttgcg cttttctcgt atgccaggcg gcggttttag cgccgggacc
      301 ggtggcggta atacttttga tggtgagtac cagcgaaagg atgatgagcg cgaccgcctt
      361 gatcataaag acgatcgcca ggattaatgt cgaaacgccg gattatgtgg ttatgccatt
      421 ttccggcgtt tttcgttttg gcagcaacag ccataacccc gccagcatga tcagcgcata
      481 gagacttttc cagccgacca ttgccagtaa cagaacgcat aacagcccgc caaccaccgc
      541 cagtagtcga taacgtcctt gcaataattt acagcctgcc agcatgcata acagataaat
      601 cataataaag atgccattgg cataaataat aagagcgtcc agattgatct ctaaagcatg
      661 aatcaccaac gtgctcacca cacagcagcc gagcaccgca ttgagggcat tattcgggat
      721 atggcgagaa gagaggcgtg ccaggtagtg gtcaggatta tgttgcgcct gcgaccagac
      781 caggcgggcg aagctctgta tataaatgtt gagactggca aagcaggcca gatagccaat
      841 cacgcaggca atccataacg ctcctacacc gaacaactgc actacaattt ttggaagcga
      901 tgctgccgcc gccatttttt caccataggc gtcgaagtgt aagacgacta ccgtacagcc
      961 ccagtagact aatcctgcca gcagcagacc aatcatcaat gcgcgaggaa aatcacgctc
     1021 tggattttta aattccgagg caagatgggc aaatgcttcc agaccgacaa aacaccagaa
     1081 catcactgat aacgcagcaa ataacccggt aagttcgata ttaccaggtg ccgggaaggg
     1141 gatattcgca ggtttgatat cgcccgccca ccagatagcg acaatcagcg cgacgataag
     1201 tccggcaata acggtttgta gattagcact ggaactggca ccgcgagtac cgatatacca
     1261 caccagcgcc agcgtaccga gttctgccaa caacagttgc cagctatgcc agccaaacat
     1321 tgcctggccg aacccggcgg caatttgtag cgcggcaggc aaacccacgg gaatgaccga
     1381 taaaaacaac cagccggtga ctcgctcaag tcgcgaacca aacgccatac cgacgaagtg
     1441 cgcgacaccg cctgcgctgg gatagtggcg acccagaatc gcaaacacaa tcgcaatcgg
     1501 gaacactaag ataatcaaaa cgggccacgc ccacaggctg ttattgcccg ctaccagcgc
     1561 agctaacgca ggaacggcaa acacgccagt gcctaataat gacgtcgata gcaggccaat
     1621 gccctgggcc agccccagtt cttgtttgag tccactcatg ggttgatatc cgattgcgcc
     1681 caaattttgg gcaactgcgt agattttcga tggtagcaca atcagattcg cttatgacgg
     1741 cgatgaagaa attgcgatga aatgtgaggt gaatcagggt tttcacccga ttttgtgctg
     1801 atcagaattt tttttctttt tcccccttga aggggcgaat cctcatcccc atttctctgg
     1861 tcaccagccg ggaaaccacg taagctccgg cgtcacccat aacagatacg gactttctca
     1921 aaggagagtt atcaatgaat attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag
     1981 aagttgaaac taaatctgct ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca
     2041 cccgcggcga agtgctggct gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc
     2101 cgctggatgt gaaagttggc gacatcgtta ttttcaacga tggctacggt gtgaaatctg
     2161 agaagatcga caatgaagaa gtgttgatca tgtccgaaag cgacattctg gcaattgttg
     2221 aagcgtaatc ctcgcacgac actgaacata cgaatttaag gaataaagat aatggcagct
     2281 aaagacgtaa aattcggtaa cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg
     2341 gcagatgcag tgaaagttac cctcggtccg aaaggccgta acgtagttct ggataaatct
     2401 ttcggtgcac cgaccatcac caaagatggt gtttccgttg ctcgtgaaat cgaactggaa
     2461 gacaagttcg aaaatatggg tgcgcagatg gtgaaagaag ttgcctctaa agcgaacgac
     2521 gctgcaggcg acggtaccac cactgcaact gtactggctc aggctatcat cactgagggt
     2581 ctgaaagctg ttgctgcggg catgaacccg atggacctga aacgtggtat cgacaaagcc
     2641 gttaccgctg cagttgaaga actgaaagcg ctgtccgtac cgtgctctga ctctaaagcg
     2701 attgctcagg ttggtaccat ctccgctaac tccgacgaaa ccgtaggtaa actgatcgct
     2761 gaagcgatgg acaaagtcgg taaagaaggc gttatcaccg ttgaagacgg taccggtctg
     2821 caggacgaac tggacgtggt tgaaggtatg cagttcgacc gtggctacct gtctccttac
     2881 ttcatcaaca agccggaaac tggcgcagta gaactggaaa gcccgttcat cctgctggct
     2941 gacaagaaaa tctctaacat ccgcgaaatg ctgccggttc tggaagctgt tgccaaagca
     3001 ggcaaaccgc tgctgatcat cgctgaagat gttgaaggcg aagcgctggc aactctggtt
     3061 gttaacacca tgcgtggcat cgtgaaagtt gctgcggtta aagctccggg cttcggcgat
     3121 cgtcgtaaag ctatgctgca ggatatcgca accctgactg gcggtaccgt aatctctgaa
     3181 gagatcggta tggagctgga aaaagcaacc ctggaagacc tgggtcaggc taaacgtgtt
     3241 gtgatcaaca aagacaccac cactatcatc gatggcgtgg gtgaagaagc tgcaatccag
     3301 ggccgtgttg ctcagatccg tcagcagatt gaagaagcaa cttctgacta cgaccgtgaa
     3361 aaactgcagg aacgcgtagc gaaactggca ggcggcgttg cagttatcaa agtaggtgct
     3421 gctaccgaag ttgaaatgaa agagaaaaaa gcacgcgttg aagacgccct gcacgcgacc
     3481 cgtgctgcgg tagaagaagg cgtggttgct ggtggtggtg ttgcgctgat ccgcgtagcg
     3541 tctaaactgg ctgacctgcg tggtcagaac gaagaccaga acgtgggtat caaagttgca
     3601 ctgcgtgcaa tggaagctcc gctgcgtcag atcgtcctga actgcggcga agaaccgtct
     3661 gttgttgcta acaccgttaa aggcggcgac ggcaactacg gttacaacgc agcaaccgaa
     3721 gaatacggca acatgatcga catgggtatc ctggacccaa ccaaagtaac ccgttctgct
     3781 ctgcagtacg cggcttctgt ggctggcctg atgatcacca ccgagtgcat ggttaccgac
     3841 ctgccgaaaa atgatgcagc tgacttaggc gctgctggcg gtatgggcgg catgggtggc
     3901 atgggcggca tgatgtaatt gccctgcacc tcgcagaaat aaacaaaccc ccgggcagaa
     3961 atgtctgggg gtttttcttt tggtcatctt tctagtataa gattcagaca cggacgacgc
     4021 gagtggcgtc cagctcattg attatgggga ataacatgca cgtaaaatac ttagcaggga
     4081 ttgtcggtgc cgcgctactg atggcgggtt gtagctccag caacgaattg agtgctgccg
     4141 gtcagagtgt acgcattgtg gacgagcagc caggcgcaga gtgccagctg attggtactg
     4201 cgacaggtaa gcaaagcaac tggctttccg ggcaacacgg agaagagggc ggttctatgc
     4261 gcggcgcagc aaacgatctg cgcaaccagg cggctgcaat gggcggtaac gtgatttatg
     4321 gcatcagtag cccgtcgcag ggaatgttgt ccagttttgt cccggcggat agccagatta
     4381 tcggtcaggt ttataagtgc ccgaactgat gtgatccaga ggctgggtga caaacgtatt
     4441 attgcctgat gcgcttcgct tatcaggcct acagcaggca ttgcaactga ttgaatgctt
     4501 gagaatttgt aggccggata aggcgtttac gccgcatccg gcatgaccaa cgatcacgtt
     4561 atcagtcatt tcaggctgga tggctccagc ctcgcattag caaagatgag attatttcgc
     4621 ctgtggtgca gttttgctgg tggattgaac acgtaagtga tgcagcgcgc gtaagcattg
     4681 atcctgggtt agcggttggg cgttattcgc cgctacggtg cgaacataga cctgacatgg
     4741 cacacccgcg agttttttca gtcgttggct gaaatcaagc attcggcgag tgatcgcctc
     4801 tatttcttta cccggcgttg cttcacgcgt tttcaccact gcgccgctaa attgttcttt
     4861 gccactttca tctttaaagc ggtaaagcac cgccagataa tggtcgaaca ggttatgttt
     4921 ccattccggt tgggaatagg tgtcatattc cagattgcca ttttcctggc agtcaacgtc
     4981 atataacggc aggaaatcta acagtgaggt aatacgtaat gccagttcac gcatttccgc
     5041 attattaatt gcatgaatga ttgctcgcgc cagcatttca atctgcaact cattgacgac
     5101 taactcacat ttgctaccgt catgtagcgt taatacgaag gtaagatttt cgccttgatt
     5161 atcggtaagt tccagcgtgt taacccgacg attaatatcg gcatttttaa gctcatcaac
     5221 cagaatttct gggatgtttt ctgccatttt cttaatgact ttatcgcgag cttgctcata
     5281 ctgaaggcgt gcagcagcat ccagtttatg cttttgatgc atacgacttt ccagcgcaat
     5341 cagcaggtcg cgcagttcca tgacagacat aaagaacaac gattctttat tgcgaggttc
     5401 tttaattttc agcgccagtg caatgaaatt attactcttg cgaataacac cggtattaac
     5461 acccttgatg cttatggcca tgtaggttct ccctaaccat ttctcaatta aataattaat
     5521 tttaatttat aagccagata aatgggcttg gtagtaatag ttgttaaaat aacataaata
     5581 gccgtactca ctctatataa acagaaaaga aaggttaatt attggtgttg gctatataaa
     5641 gagtaacaac agcaatgtat atgaataatt aaaggttatg caacgggcaa agattaaatt
     5701 tcccagttta atgacaggtt gctgtacttg cgtttgttgc tcttgccgga tgcggcgtaa
     5761 acgccttatc cggcctacag ttcattgcaa atccattcaa tttcaatgca caatgtaggc
     5821 ctgataagcg tagcgcatca ggctaatgtt taacttccct gtttaatcag ttactgctgg
     5881 cgtagctgga gatccagcgg cgttttgctg ggttcgccgc cgatttctcg cgccagtttc
     5941 ggcaccagat aacccgacac cagtgtcagc aactcacgca taatctgccg tgcttcgtca
     6001 tcactcacca taaaatgcgc cgcgccctgt actttatcta gcacatgcag gtaatacggc
     6061 atcacgccgg catcgaacaa cgcattactc aggtttgcca gcgtttgtgc gttatcgttc
     6121 acaccacgta acagaacgct ctggttaagc agggtgacac cgacacggcg caatttagcc
     6181 atcgcctgac ggaacgtttc atctacctca ttggcatgat tgatgtgatt caccagcaag
     6241 atttgcagcg tagaacgggc aaagcgttca accagcgcct cggtgatacg tgccgggatc
     6301 acaatcggca gacggctgtg aatccgcaga cgtttgatat gcgggatggc ttccagttgt
     6361 gtgagcaacc agtccagctc gtgatctttc gccatcagcg gatcgccgcc ggagaaaatc
     6421 atctcgtcca gttccggatg cgcagcaaca tactcaagag cagtctgcca gttacgcttg
     6481 ttgccctgat tttcggcata ggggaagtgg cgacggaagc aatagcggca atttaccgcg
     6541 cagccgcctt tgaccagcaa aagcgcccgg ttgtggtatt tatgcaacaa accaggcact
     6601 acgctgtgct gttcttccag cgggtcggtg gagaatccgg acgcgacgac aaactcatct
     6661 tgcgaggtaa gtacctgacg caaaagagga tcgtccggat tgcctttctc catgcgatcg
     6721 ataaatgagc ggggcacacg cagggcaaac agctttttgg cgctgcgtcc ggccaacagt
     6781 ttttcgtccg cgtctatatt caaaagacgc agaagttcat caggatcggt cacaacatcg
     6841 gcaagttgcg ttaaccaatc ttctctggat ggggtattta gggttacaat atgcgccatt
     6901 ttgtggctta gctaccaatt aacaaatttc agagggcctt atggcaacgt actatagcaa
     6961 cgattttcgt gctggtctta aaatcatgtt agacggcgaa ccttacgcgg ttgaagcgag
     7021 tgaattcgta aaaccgggta aaggccaggc atttgctcgc gttaaactgc gtcgtctgct
     7081 gaccggtact cgcgtagaaa aaaccttcaa atctactgat tccgctgaag gcgctgatgt
     7141 tgtcgatatg aacctgactt acctgtacaa cgacggtgag ttctggcact tcatgaacaa
     7201 cgaaactttc gagcagctgt ctgctgatgc aaaagcaatt ggcgacaacg ctaaatggct
     7261 gctggatcag gcagagtgta tcgtaactct gtggaatggt cagccgatct ccgttactcc
     7321 gccgaacttc gttgaactgg aaatcgttga taccgatccg ggcctgaaag gtgataccgc
     7381 aggtactggc ggcaaaccgg ctaccctgtc tactggcgct gtggttaaag ttccgctgtt
     7441 cgtacaaatc ggcgaagtca tcaaagtgga tacccgctct ggtgaatacg tctctcgcgt
     7501 gaagtaatgc ggttgtggtg cggcctgcag gctgcaccat cacttattca ggtcagagat
     7561 gatgaaacgc cttatcgttc ttgttttgct tgccagcacg ctgctcacgg gctgtaacac
     7621 cgctcgcggt ttcggcgaag acatcaaaca tctcggcaac tccatctctc gcgctgccag
     7681 ctaatttttc ttctcttccg aaaaatcatc agatttccat catttttggc gatgttgtct
     7741 attattaatt tgctataggc aaacataaat aacattacct aaaaggaaga cgttatggtg
     7801 aagaagacaa ttgcagcgat cttttctgtt ctggtgcttt caacagtatt aactgcctgc
     7861 aacaccacgc gtggcgttgg tgaagacatt tctgatggcg gtaacgcgat ttctggtgca
     7921 gcaacgaaag cgcagcaata agcaataacg gtacgacagt tatgtcgtgc cgtttgtttt
     7981 tttgcgatag tcacaaaggt aatagttgaa attcccctgc aacctggcaa aatatccgtt
     8041 caaccatcag ctttgcagga cgacctgcaa acgcctcttt tcaccgggga cggccccaat
     8101 tctccggagc ctgatatgtc ctggattatc ttagttattg ctggtctgct ggaagtggta
     8161 tgggccgttg gcctgaaata tacccacggc tttagtcgtt tgacgccaag tgttattact
     8221 gtgacggcga tgatagtcag tatggcgcta cttgcctggg cgatgaaatc gttaccggta
     8281 gggacggctt atgccgtgtg gacgggtatt ggcgcagtcg gcgcggccat caccggcatt
     8341 gtgctgctcg gtgagtccgc taacccgatg cgcctggcga gtctggcgtt aatcgtattg
     8401 gggattattg gtctgaaact cagcactcac taactaccgg gctgctgtac ccaaataaac
     8461 ttactgacat caaacccttc ccgggtcgcg actgccagca tctcctgttt cacttcgtca
     8521 gaaatggttg gcgtgcggga gagtatccac aggtagtcgc ggtccgggcc gcaaaccagc
     8581 gcatggcggt attcccgatc gagtgcaata acgttataac cgccatagaa aggaccaaag
     8641 aatgacactt tcagcgcagc gcgagttggt gcgccggtaa agtacgcttt cccttcactc
     8701 tgctgccaca ttcctctgtc agggttatag cctttattaa tgacattcag gccgccgtca
     8761 tcacgcaggc tgtatgttgc ggtgactttt tccagtccac gttcaaaacg gtgatcaaaa
     8821 cgggcaatct cataccaggt accgagataa cgtttggcgt cgaaattatt cactactgtt
     8881 acgccacgcg gcggcgtagg agaactgcag gcaacgacca gaaatgcagc tgtcgctgcg
     8941 gcaacgagag ggagcaggcg cataaatgtt tccttactgg tttttttcta agtgtagatg
     9001 acagcaagga aaagcgcaga aaaggtccga aaattcggac ccgatgaaat tttactgtag
     9061 agcgttgaga atctgccagg cggcggcgac tctcgctgga ttgggatagt ttttgttagc
     9121 cagcatcact atacccagct ctttttctgg aataaacgcg acatagctac caaatccgcc
     9181 ggttgcccct gttttatgta cccatgatgc gcgtactgca ggagttgggg gcgtaatcgc
     9241 ttttacgggg tgtgctgcca gtgcaatttt attgccactg ccgttaatga tgctgtcagg
     9301 atttaccggc cagtccagca tttcccagcc caggccctga tacatatcgc cggtttgcca
     9361 gtagcgagat tgtgccagtt gtatcccttg ctgaagtgtt ttgtcgttga tatcacgggg
     9421 attcatattg cttcgtaccc agcaggccat atcttcaatg gtcgacttca caccataagt
     9481 ttcagcatct aacgcccctg gcgaaacatg aactgccttg ccttcgcgat atccccaggc
     9541 gtaattcttt tcttctgcgg gcggtacatt aatccacgta tggttgagtt tgagtggctg
     9601 gaagacacgt gtttgcatcg cctgctcaaa actcaaacca gacggcttca cagccagtgc
     9661 gccgaacaaa ccgatactgg agttggcata cagacgttgt gttcctggcg cccatgcagg
     9721 ctgccagttt tgatagaagc gcagcaagtc gcttgaggat ttcacctcat ccggcacctg
     9781 caatggcagg ccgccagcag tgtaggttgc gagatgtaat agtgtgatcc cattccactg
     9841 tttagcggta agttcaggcc agtattttgt tgtgggatcg cttaacttga tttcccctcg
     9901 agcaatagcg tcgccaccaa gcacgcccgt aaatgttttg ctgaccgaac ctaactcaaa
     9961 caacgtttgc tgtgtgacgg gctgcttttt ggcgatgtcc gcatagcccc aggtaaagta
    10021 ataaggttta ccctgataaa ttaccgccac cgccataccg gggatctttt gttgctctat
    10081 aagcggggta attgtgcgat gcacaatatc gttgatttgt tgaggggcag caaatgtgga
    10141 gcaagaggcg gtaattaata aggcgcagag cgtcgttttg aacataaggt ctggtttcca
    10201 tacaaaacgg cccgccatag gcgggccgga tttacattgg cgatacgtta gattgtaacg
    10261 ataccaatca gcgtgacaac tgtcaggata gcagccagac cgtagaaaac ccatttgccc
    10321 gcaggcacgt gaattttcag atcgtgcatc gcgtggtgca tacggtgtaa accacaccac
    10381 agcggcagaa cgatcatcag gaacaggaat acgcgaccaa tgaagctctg cgcgaacgcc
    10441 agaacgcgct cgtagctcag cgcatcaccc ggaaacagcc ccagtggcag cagaataccc
    10501 accagcagga tcatcaccgg cgcaataatt gcgctccaca taccaccggc cccgaagagg
    10561 ccccagaata ccggttcgtc agaacgcttt ggatttggat taatcatctc aggctcctta
    10621 ccagtacagg gcaacaaaca ggattacgat ggtggcaacc acagttaccg cccagagact
    10681 tttgataatt ggctctggtc ccattttttc gtcttttaca atgatattgg ctgctttcgg
    10741 tgccagttca aaccaggttt tggtatgcag cagagctgcc gccagagtga tcaggttaat
    10801 gatcacgata accgggtttt gtaaaaagtc gacgaatccc gcccaggctt ccgggccatt
    10861 tttcagggca aacagcccga aaatcagttc aatgctgaac cacacagccg gaaccgccgt
    10921 gccttcgcgc agcatgtaaa agcgataaaa cggcaatttt ttccaccagg tggacgtcat
    10981 tggccgtaca tacggtttac gtttagtcgt catgttgcac tccttagcgt ggtttcaggg
    11041 tcgcgataag aaagtctttc gaactttcta ctttgccctg ctgaatggcc gcagccggat
    11101 cgacgtgttt cgggcagact tcggagcaat agcccacgaa agtacagctc catacgccgt
    11161 tctggctgtt caactgcgcc atacgctcct tcttaccgtg gtcgcggcta tcttcgttat
    11221 aacgatgcgc cagcgtaatg gcagccggac cgatgaactc tgggttcagg ccaaactgcg
    11281 ggcacgcggc gtagcacaga ccacagttga tgcaaccgga gaactggtga tactttgcca
    11341 tctgcgccgg ggtctggatg ttagtaccct gatccgcggt gcgggagttg ccgatgatgt
    11401 acggtttgat cgcttccaga ctttcgatga agtgggtcat atcgaccacc agatcgcgtt
    11461 caatcgggaa gttagctaac gcttcaacct tcataccgtc ggtgtaatca cgcaggaagg
    11521 ttttacatgc cagttttggc acgttgttaa ccatcatgcc gcaggaacca caaatcgcca
    11581 tacggcagga ccagcggtaa ctcaggtccg gtgccaggtt gtctttgatg tagcccagcg
    11641 catccagtaa tgaggtagtt gcgtcataag gcacttcata gaatgcgcta tgcggtgcgg
    11701 tatcgacttc cgggttatag cgcaccacct caattttcag gtttttcatc tcagccattc
    11761 gccttctcct tcttattggc tgcttccgcc ttatcggctg catccgcttc gccaccgtaa
    11821 acgcgtttag ctggcggcag cgtagtaatc ttcacgtcgc tgtactccag gcgagtcgtg
    11881 ccatcagcat cgcggaaggc gagggtgtgt ttgaggaagt tgacgtcgtc acgctcggtg
    11941 caaccttcgt ccagacgctg gtgtgcgccg cgggactctt tacgtgccat tgcggagtgc
    12001 gccatacatt cagcaacgtt cagaccgtgg cccagttcaa tggtgtagag caggtcggtg
    12061 ttgaacacgc tggaggtgtc ggtgatgcgc acgcgcttga agcgttcctg cagctctgcc
    12121 agcttgtcga tggttttctg catcagttcc ggcgtacggt agataccgca accttcttcc
    12181 atagccagac ccatttcgtc gcggatcttc gcccagtttt cgccgccatc ctggttaacc
    12241 agatctttca gacgttgttc aacgccagct gcctgcgctt caattgccgc ttcgttgcca
    12301 ttaccggcag ttgctgcacg ctctgtcgct tgttcaccgg ccagacggcc gaagaccacc
    12361 agttccgcca gggagttgga gcccagacgg tttgcaccgt gcagaccaac agaggaacat
    12421 tcacccacgg cgaacagacc tttaatgcgg gtttcacagt tctgatcggt ttcgataccg
    12481 cccatggtgt agtgtgcggt cggacgtacc ggaatcggtt ctttaaccgg atcgacgcca
    12541 acgtacgctt tcgccagttc gcagatgaac ggcagacgtt catgcagttt tttctcgccg
    12601 aggtgacgca ggtcgagata aaccacatcg ccacgcggcg tggagatggt gttgccttta
    12661 cgccattcgt gccagaaggc ctgagaaact ttgtcgcgtg gacccagttc catatatttg
    12721 tttttcggct cgcccagcgg agtttccggg cccatgccgt aatcttgcag ataacggtag
    12781 ccatttttgt tgaccagaat accgccttca ccgcggcagc cttcggtcat caggataccg
    12841 gaacctggca gaccggttgg gtgatactga acgaattcca tgtcacgcag cggaacgccg
    12901 tggcttagcg ccatacccat accgtcaccg gtaacgatgc cgccgttggt gttgtaacga
    12961 taaacgcgac ccgcaccgcc agtagccata acgaccgcgt tagcacggat ctgcaccagc
    13021 gtgccttcca tcatgttcat tgctaccagg ccgcgaacat gaccatcatc aaccagaata
    13081 tccagcacga aatgttcgtc aaaacgctgg atctgcggga attgcagaga ggtctggaac
    13141 agcgtgtgca gcatatggaa gccggtctta tcggcggcga accaggtgcg ctcgattttc
    13201 atgccgccga agcgacgtac gttgacgcta ccatccgggc gacggctcca tgggcacccc
    13261 cacagttcca gttgggtcat ttcggttggg cagtggtgga cgaaataatc cacgacatcc
    13321 tgctcacaca accagtcgcc acccgctact gtatcgtgaa agtgatattc gaagctgtca
    13381 tgatcctgcg cgacagcggc ggagccccct tctgcagcaa cggtatggct acgcatcggg
    13441 tatacttttg agattagtgc gatttttgca tttggatttg cctgcgcggc agcaattgca
    13501 gcacgtaatc ccgcgccacc ggcgcctaca atggcaagat cggcttgaaa ggtttgcacg
    13561 acattcctcc agattgtttt tatcccacag ccccgtactt cagggtaagt gcctgaaagt
    13621 tacggtctgc gaacgctatt ccactgctcc tttataggta caacagtata gtctgatgga
    13681 taagtctgaa atttgacgag atcgattttt ttagtgcgca agagggctaa attatcactg
    13741 aagatgatta atttaattac taaaccatca gatcgtgctt tttttagtca ctgcccaccg
    13801 ctgtttgatt cctgcgtaaa atttgtctcg ccgtcgcgtt gcgagtagac ttcgtgccct
    13861 tgtcaaaaac tggagattta actatgagcg aaacggcatc ctggcagccg agcgcatcca
    13921 ttcctaactt attaaaacgc gcggcgatta tggcggagat ccgtcgtttc tttgccgatc
    13981 gtggagtgct ggaggtggag acgccttgta tgagccaggc gacggtaacc gatattcatt
    14041 tggtcccgtt tgagacacgt ttcgttggcc ccgggcattc gcaggggatg aatctctggt
    14101 taatgaccag cccggaatac catatgaaac gcctgctggt tgccggttgt gggccggtat
    14161 tccagctgtg ccgcagcttc cgtaatgaag agatggggcg ttatcacaac cctgagttca
    14221 ctatgctgga gtggtatcga ccgcactatg atatgtaccg gttgatgaac gaggtggacg
    14281 atctcttaca acaggtgctg gactgcccgg cagcagaaag cctttcttat caacaagctt
    14341 tcttgcgtta tctggaaatt gacccgctct ctgccgacaa aacgcaactg cgggaagtcg
    14401 cagcgaaact ggatttgagc aatgttgctg ataccgaaga agaccgcgac acgctgctac
    14461 aattgctgtt tacctttggc gtagagccaa atattggcaa agaaaaaccg acctttgtgt
    14521 accactttcc agccagccag gcatcactgg cgcaaatcag taccgaagat catcgggtcg
    14581 ctgaacgctt tgaggtttat tataaaggta ttgagctggc gaatggtttc catgaattga
    14641 cggatgcccg tgagcagcaa caacgctttg aacaagataa ccgtaagcgc gcggcgcgcg
    14701 gtttgccgca gcaccccatt gaccagaatc tgattgaagc cttgaaagtc ggtatgcctg
    14761 actgttccgg cgtggcatta ggtgttgatc gtctggtgat gttggcgctg ggcgcggaga
    14821 cactggctga agtcatcgcc tttagcgttg accgggcata attctgaaaa attactgaat
    14881 taacagcgaa gaatggcgtg ataagcgcca ttctgtagca tatgttttca tttttccgtt
    14941 aaaggccaca atttaagact ggatatttga tatcatccag gtatcaattc tgtattggtt
    15001 tttacgctgc ccgctcaatg ttgggcagtg cagttcagtg ggcgcgtttt atacacgcgc
    15061 tgaaatgaag gatggtttca tgcctcacac gataaaaaag atgagtctga taggactcat
    15121 attgatgatc tttacttccg tatttggatt tgccaatagc ccatcggctt attacttaat
    15181 gggttatagt gcgattccct tttatatatt ttctgcattg ttattcttta ttccattcgc
    15241 cttaatgatg gctgaaatgg gagctgccta tcgcaaagaa gaaggtggta tctattcctg
    15301 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
    15361 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
    15421 ctatggtagc gacatgaccc agcactggcg tattgctgga ctggagccta cgcaggtggt
    15481 tggtctgctg gcagtggcat ggatgattct ggtcaccgtc gttgcttcaa aggggattaa
    15541 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
    15601 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
    15661 tttccttgca tcaccgaacc caggttatca gtccggtctg gcaatgctat cgtttgtggt
    15721 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
    15781 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
    15841 ttattcgctg gcaatatttt tatggggtgt cagcacaaac tggcagcagg tattaagtaa
    15901 tggttccgtt aacctcggca atattaccta tgtgctgatg aagagcctcg gggtgacgct
    15961 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggtgtat ggtttgcgcg
    16021 tattaccgga ctttcgatgt tcctcgctta taccggggcg ttctttacgc tttgctattc
    16081 accgctgaaa gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg
    16141 cctgaatgcg atggggatgc cttctatcgc catgtggatg cagtgcgggt tggttactgt
    16201 cttcatcctg ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac
    16261 gctgatggcg aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt
    16321 ctttaaagca cgtcaggatc tcgacagacc gtttgtgatt ttcaaaacgc atttgtcggc
    16381 aatgattgcg acagtggttg tcgtactggt ggtgacattt gcgaacgtct tcaccatcat
    16441 tcaacctgtg gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt
    16501 cttcttctcg ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa
    16561 acctgagtta gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat
    16621 gagttgtcag gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat
    16681 gatgttaagc tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac
    16741 ttcccgcctg acgacctttg cccgcagaag ttaatgttct ccccgtttgc ttaccgttga
    16801 ggctttccag acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc
    16861 ctgccagaat cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg
    16921 cgtaaatacg cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg
    16981 gtgccgggtt gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca
    17041 cttcttcgct attggcatcg gcaggggccg gtatcgtcaa caccacgcgc gtgaccgagt
    17101 cagagagcga ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct
    17161 ctttacggtc ccagtcgctg atggtggtgg cgcgggtgtt aattttcgtc acgctaccgg
    17221 taagatcgcg gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggataatca
    17281 ggccagagat aaagttggcg aaaatctcct gcaaaccaaa accgagacca acaccgagcg
    17341 cggcaaccag ccactgcaat ttcgaccact caataccaat catcgagaag ccgaccagcc
    17401 cgccaatcag catcagcaga tatttggtga tggtggtgat ggcgtagccc gtacccggcg
    17461 ttaaatccag gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct
    17521 gcgtggtgat gataaacacc agaatggcaa tcagcaccgc accgagggta attggctcca
    17581 gactttctac gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc
    17641 cgaaagcgga atggatttct gaccacagca caatgacaga aagcagggcg atcaacatca
    17701 aaattgagcg caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt
    17761 catcgacttc aattgcccct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg
    17821 cgcgttgcgc taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac
    17881 gctggatcag catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg
    17941 caaccgaggt ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgctg
    18001 acgccagaat ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa
    18061 tgttgtcgcc gctgccctct ttgttgaggt acagcgggat cccggccttt ttcaggctga
    18121 gggtgaccac cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg
    18181 agaattcacg gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ggcacaataa
    18241 gcccgatgct catcaggtag tagcgcatcc cacgggaaac acgttcgcgc ggccagccaa
    18301 aatgagcgat aaacaagccg ttcgggcggg caaaggtggc gcaaatcatc actacccaca
    18361 gtagcggcac ggtggccgtt acgccatcgc caatcgcgac cgccagcgga taaggccacg
    18421 cctcgcgcaa gccgtaaccc agcgtcatcc acagcaccgg cagcggtgac gcgacgagaa
    18481 tcgaccagaa aagagtgcgc aacgtcagcc agaagtgatc ctgagtcact ttgccgactt
    18541 tcgccgccga acgttcaaga aaacgggtga aatagcggcg cgagtaaata ctgcaaccga
    18601 ccagaatcaa cgcgccaaac agcggcaaaa tcgtctcttt gctggtcagc atcatcacgc
    18661 tggctttacc caactgactg aaggtgtcca gcgagatgag gcggcgcaga tcctgggcga
    18721 tttccagcgg ccaggcgata gtcatcgggc gcacgtcaga ggtccagaac agatagcggt
    18781 gcgtcgcttc gttcacctct ttcagcgcat cctccagttg cccgttggag actttcagct
    18841 tggtcagttc cagcagtagc gtgtctccac cctgcaacaa tgagttcagc aactcacgct
    18901 gagtgcgtag ctgtgcttcc agaatacggt tttgctcggc ggtcagcggc tgaccgtcgg
    18961 cctgatgaat ttgccttagc tgcggctgtt tattaagcag atcctcataa cgtaaccgtt
    19021 gcacacgcaa ctgcgccatt tcggtatcaa gttgttgtgg ttttggcatt tccggcagcc
    19081 gtgccacctg cgcccgcaac gcttcgccga gcaggttgga cgagcccagc cattgcgact
    19141 gttcacgcag cgtattcaac gcctgccgga cctgtaacgt ctggctggtg gcctggcgct
    19201 gttgcgaggc aacgagatcc atccgctgcg cctgttgatt cagtgccgcc gatagttcgc
    19261 ggttaatttt gaattgcgcg acgatatctt tcggcaaatc ggcactgttt tctgccagta
    19321 attcggtact ttccagcgcc cgctccgcct caagctgacg ttggctattc aactgattac
    19381 gtaaggcctg caaatacgca tccagttgct ggctctcttt ttccgccagc tctgagcgca
    19441 agcgcgctaa ttcctgacgg ttattggcag acagctgcgc cagttccagt tcatcaacga
    19501 gcgctttcag gcgtgcggag tcagactgca acgcgaaatt ttgtgcctga ttgagcggag
    19561 tattgccggt aagcgttccc aggcggcgct cgatctcatt taactgacgg cgggcatcgg
    19621 tttgctgttg cggcagttga ttcagtgaat cggcaatctc gcgggcgcgc tcctgctctt
    19681 gctgggcctg acggctctta tccagcaact gactgctgac ctggagaatt tcctgattca
    19741 gcgcatcggt agacattccc ggcgacacgc tgcgcggctc gtcacgcatg ttgtttaatt
    19801 gtgcgcgcag agtagcggag agtttcgggt aattatcgat aacttgctga tattgtttga
    19861 tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa ggcagactgg agtgcctcta
    19921 tgacttctgg ctgcgcgggt ttcgccgctt ttgcctgctc cagttcctgg gtgatttgtt
    19981 tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag gcaccaggcc atcagaaaag
    20041 tgataatcag gcgcacgtca gcgtttcctt tgatggatta gacctggtct tttttgtcgt
    20101 caaccaatgg gctggcgtcg tgttctgctt cgatctcttc agcaggaagc ggggcaggtt
    20161 cagcgtctgg cgtaacaaag gtttcggtag atactgccag cggctggcca attttcgtga
    20221 cagacaggct ttccagttgc tcaaccagat tcactttacc cggtgcaaac aggttgataa
    20281 cggtggaacc gagtttaaag cgacccattt cctggccttt aagcagtgcc acagaaccgt
    20341 cgttttcccc ggcaggccag gtccagcgct tgatgatacc ttcgcgcggc ggcgtaatgg
    20401 tgcccgccca gaccgtctca atgctgccaa caatcgtcgc tccgaccaga atctgcgcca
    20461 ttgggccaaa ttcggtatcg aaaaggcaaa tcacgcgttc gttacgggca aacagattcg
    20521 gcacgttctg agcggtgaga tggttaacgg agaagagatc gcccggcacg tagatcatct
    20581 cacgcagaat accgttgcac ggcatgtgta cgcggtggta gtcacgcggg gagaggtaag
    20641 tggtcacaaa cgtaccgttg cggaacaggt ccgccatcag atagttgcct gccagcaagg
    20701 cttcgaggct gtagttgtgg cctttggctt gcaggatttt atcttcttcg attttaccca
    20761 actggctgat aacgccatcg gcaggcatga ccaggacatt cggatcggta tcgattgggc
    20821 gtacttcgtc acgcagcgga cggacaaaga attcgttaaa ggtgcggtag ctggcggtgt
    20881 ccggcttttg cgcctctttc atgtcgacct tgtagtattt aacgaacaga tcgataacca
    20941 gttttgtcag ccatcctgcc cgcttgcttg cgccccaacc cgccaggcga gtaagccata
    21001 gtttcggcag aatgtactgt agcgaaagtt taaatgaatt taacaaggta gcctccaggc
    21061 cattgttttg tcgttcctga tccggcctac atgccggatc ctgaaaaaaa ggggacgatt
    21121 ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga aaagttttta cgcgttttta
    21181 cctgcgccat gctttccaga atacggtgat agttttcgaa acgggtttcc gcgatttttc
    21241 cttcctcaac cgcttcccgg atagcgcagc ccggatcggt atcgtgtttg caatcgcgat
    21301 atttacacag acctaaatag tcatggaatt cgacaaagcc ctgagtgatt tgttccggct
    21361 ccaggtgcca gaggccgaac tcacgcacac caggggaatc aatcacatca ccgccgtgcg
    21421 ggaagtgata cagtcgtgcg gcggtcgtgg tgtgctgacc gagacccgag ttgtcagaga
    21481 catcgttggt caggatctct ttttgcagcc ccagcaacgc gttcagcagg ctggatttgc
    21541 cgacgccaga ctggccggca aaaatgctga tgcgcccggt caacgcctct tccagcggtt
    21601 ttagcccatc ctgagtatgg ctggaaacca tcaatacgcg ataaccaatg ttgcggtaga
    21661 tatccatctg ctcgttgacg aacgccatgc cttcgtcgtc cagcaggtct atcttattga
    21721 gcacaataat cggctcaatc tgcaaggttt cgcaggccac caggtaacgg tcgataatat
    21781 tgagcgacag ctccggcaaa atggcggaga caatgacaat ctggtcgatg ttggcggcaa
    21841 taggtttcac gccgtcgtag aaatccggac gcgtcaacac tgaggtgcgc tcatgcaccg
    21901 cttccacgat ccctttgaca tttacgcctt ccgccgccgg tttacccgga cgccagacta
    21961 cgcggtcgcc ggttaccagc gaacggatgg tacggcgaat attgcagcgg tgaacgtcgc
    22021 catcggcgga ttccacatca gcgtgcatac caaagcggct gatgacgata ccttcatcag
    22081 gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt agacgtttta agacgacgct
    22141 ggtgattggc gttcacgcgg cgctgctggc ctttggagag tttattttta ctcaatcgta
    22201 cagactcctg gtcgcccctg atgggcaaaa catctatgat acacgcaatt gtggatcaat
    22261 atagtcactg tgaatgggtg gaaaatagca tgagtgccaa tgaaaacaac ctgatttgga
    22321 tcgatcttga gatgaccggt ctggatcccg agcgcgatcg cattattgag attgccacgc
    22381 tggtgaccga tgccaacctg aatattctgg cagaagggcc gaccattgca gtacaccagt
    22441 ctgatgaaca gctggcgctg atggatgact ggaacgtgcg cacccatacc gccagcgggc
    22501 tggtagagcg cgtgaaagcg agcacgatgg gcgatcggga agctgaactg gcaacgctcg
    22561 aatttttaaa acagtgggtg cctgcgggaa aatcgccgat ttgcggtaac agcatcggtc
    22621 aggaccgtcg tttcctgttt aaatacatgc cggagctgga agcctacttc cactaccgtt
    22681 atctcgatgt cagcaccctg aaagagctgg cgcgccgctg gaagccggaa attctggatg
    22741 gttttaccaa gcaggggacg catcaggcga tggatgatat ccgtgaatcg gtggcggagc
    22801 tggcttacta ccgcgagcat tttatcaagc tgtaaaattt ttcctggtca cgtaagcgcc
    22861 ggaatccact tgcggcggcg tgattttgac gctaaattga acgtcttgct gataatttca
    22921 gcacttgaga taaaaacgca aaaaaaactt ttttgggggg ttgcagaggg aaagatttct
    22981 cgtataatgc gcctcccgta acgacgcaga aatgcgaaaa ttacgaaagc aaaattaagt
    23041 agtacgcggg aatagctcag ttggtagagc acgaccttgc caaggtcggg gtcgcgagtt
    23101 cgagtctcgt ttcccgctcc aaaatttg
//
LOCUS       DAFATA010000070        22938 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFATA010000070 DAFATA010000000
VERSION     DAFATA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.071"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(44..754)
                     /gene="nlpE"
                     /locus_tag="LGJ20_003204"
                     /gene_synonym="cutF"
     CDS             complement(44..754)
                     /gene="nlpE"
                     /locus_tag="LGJ20_003204"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBK2387227.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(768..1190)
                     /gene="arfB"
                     /locus_tag="LGJ20_003205"
     CDS             complement(768..1190)
                     /gene="arfB"
                     /locus_tag="LGJ20_003205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK2387228.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(1187..1732)
                     /locus_tag="LGJ20_003206"
     CDS             complement(1187..1732)
                     /locus_tag="LGJ20_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBK2387229.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            1898..2098
                     /locus_tag="LGJ20_003207"
     CDS             1898..2098
                     /locus_tag="LGJ20_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBK2387230.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            2085..2345
                     /gene="rof"
                     /locus_tag="LGJ20_003208"
     CDS             2085..2345
                     /gene="rof"
                     /locus_tag="LGJ20_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBK2387231.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(2394..3692)
                     /gene="tilS"
                     /locus_tag="LGJ20_003209"
     CDS             complement(2394..3692)
                     /gene="tilS"
                     /locus_tag="LGJ20_003209"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK2387232.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(3757..4146)
                     /locus_tag="LGJ20_003210"
     CDS             complement(3757..4146)
                     /locus_tag="LGJ20_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK2387233.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(4203..6344)
                     /gene="ldcC"
                     /locus_tag="LGJ20_003211"
     CDS             complement(4203..6344)
                     /gene="ldcC"
                     /locus_tag="LGJ20_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBK2387234.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(6443..7402)
                     /gene="accA"
                     /locus_tag="LGJ20_003212"
     CDS             complement(6443..7402)
                     /gene="accA"
                     /locus_tag="LGJ20_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK2387235.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(7415..10897)
                     /gene="dnaE"
                     /locus_tag="LGJ20_003213"
     CDS             complement(7415..10897)
                     /gene="dnaE"
                     /locus_tag="LGJ20_003213"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK2387236.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(10934..11530)
                     /gene="rnhB"
                     /locus_tag="LGJ20_003214"
     CDS             complement(10934..11530)
                     /gene="rnhB"
                     /locus_tag="LGJ20_003214"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK2387237.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(11527..12675)
                     /gene="lpxB"
                     /locus_tag="LGJ20_003215"
     CDS             complement(11527..12675)
                     /gene="lpxB"
                     /locus_tag="LGJ20_003215"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBK2387238.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(12675..13463)
                     /gene="lpxA"
                     /locus_tag="LGJ20_003216"
     CDS             complement(12675..13463)
                     /gene="lpxA"
                     /locus_tag="LGJ20_003216"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBK2387239.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(13467..13922)
                     /gene="fabZ"
                     /locus_tag="LGJ20_003217"
     CDS             complement(13467..13922)
                     /gene="fabZ"
                     /locus_tag="LGJ20_003217"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK2387240.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(14027..15052)
                     /gene="lpxD"
                     /locus_tag="LGJ20_003218"
     CDS             complement(14027..15052)
                     /gene="lpxD"
                     /locus_tag="LGJ20_003218"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBK2387241.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGNNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(15056..15541)
                     /gene="skp"
                     /locus_tag="LGJ20_003219"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(15056..15541)
                     /gene="skp"
                     /locus_tag="LGJ20_003219"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBK2387242.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(15663..18095)
                     /gene="bamA"
                     /locus_tag="LGJ20_003220"
     CDS             complement(15663..18095)
                     /gene="bamA"
                     /locus_tag="LGJ20_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBK2387243.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(18125..19477)
                     /gene="rseP"
                     /locus_tag="LGJ20_003221"
     CDS             complement(18125..19477)
                     /gene="rseP"
                     /locus_tag="LGJ20_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBK2387244.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(19489..20346)
                     /gene="cdsA"
                     /locus_tag="LGJ20_003222"
     CDS             complement(19489..20346)
                     /gene="cdsA"
                     /locus_tag="LGJ20_003222"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK2387245.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(20359..21117)
                     /gene="ispU"
                     /locus_tag="LGJ20_003223"
                     /gene_synonym="uppS"
     CDS             complement(20359..21117)
                     /gene="ispU"
                     /locus_tag="LGJ20_003223"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBK2387246.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(21306..22502)
                     /gene="ispC"
                     /locus_tag="LGJ20_003224"
     CDS             complement(21306..22502)
                     /gene="ispC"
                     /locus_tag="LGJ20_003224"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBK2387247.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(22594..>22938)
                     /gene="frr"
                     /locus_tag="LGJ20_003225"
     CDS             complement(22594..>22938)
                     /gene="frr"
                     /locus_tag="LGJ20_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK2387248.1"
                     /translation="RSMSPAVEKAIMASDLGLNPNSAGSDIRVPLPPLTEERRKDLTK
                     IVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDVQKLTDAAIKKIEA
                     ALADKEAELMQF"
BASE COUNT         5441 a         6474 c         5563 g         5460 t
ORIGIN      
        1 agcgcatcag gcaattttgc gtttgtttct gccttaagca ggtttactgc cccaaactac
       61 tgcaatcctt attcgggtaa aatttacccg ccgtatcggg cgctaatact ttggttggcg
      121 caccagtatc cggattagcc tcaagcgtaa agtgaccttc tactgacagt aacaccggtt
      181 tttcactgtg accgcgcgca gccaggtagc tacgctccag ctctgcgtta ttcgctacca
      241 tgaaacgttt tccggtcgcg caatcagtga aggtcgccgc atcagccata taaaaataca
      301 tgcctcgcag ggtcatcggc gtcataggta aactagattg tgccggttcc agcgtatagt
      361 tgaactgcga ttcaatcgga ttgccttcac gatcgagcat ctccagcgca tcgcctttcg
      421 cccgataata tgacttttca cctttgctgt cggttaatac cagcttgtca gcggttcgcg
      481 cccatgtacc gtaggaagcg aaggaggaag gttcttcacg agcaccgaga taacgctcat
      541 tcatcaccca tgttccgtct ttttcgagga acagagaggt ttcgattcct tcgcaatcag
      601 cacacggcag cacgccgcgc caactttgcg gcatcggttt cagttcggca gcctgtgctg
      661 gagaaagcgt atcgacttcg gcccggttat tacatcccat cagagtaaag aggctgatta
      721 cagccatcgc tgtcactatc gcttttttca ccatctattc cttcttttta ttcccgaccg
      781 ctgcgtactt tgccgcgcat cgccttcacg cttgattttt gtgctttcga tgccagcctg
      841 cgctctttcg atgcacgggt gggccgcgtg ggtcgtcggg ctttttgttc tgttgttaaa
      901 tctttaatca ctgccaccag ccgggctagc gcggcttcgc ggttcagttc ctgactgcgg
      961 tattcctgtg ccttaatgac aatcacgcca tcactgctga tcaaatgatg gctggcggcg
     1021 agtagacgct ctttgtaata ctctggcagg ctggacgccc gaatgtcaaa acgcagatga
     1081 atggccgttg aggtcttatt aacatgctgc ccgcccgcgc cctgcgcacg aatggcggtg
     1141 atctcaagct caccatcggg aatagcaaca tgtcgggaaa tcacaatcat gaaggttgtt
     1201 gccaggcggt taagttaact tccagattat tcttatcgtc cgataaccag atcacgccat
     1261 cctgaatcgt tgcctgcagc gtcatggtac gatcggcaaa ggcgcttact ttcgccagtt
     1321 gttcatcgtc cagataccag acggaaagat tggcaaactg cacacatttg ctctgatttt
     1381 gctgccacca gatttgcgcc gcccgactat tataggtaaa cagcgccact tctgcggcct
     1441 gggtgcaggc tttcttaatc cgccgctcat ccggcagccc cagctcaatc cacaaatcaa
     1501 tgcccaggtg atcgttacgc agccacgctt ccggctcatc atcggcacac aaaccacggg
     1561 taaattgcag acgttcatca gcatatttca gccacgccag caagcgcagc atcatacgct
     1621 cctgggtttc tgaagggtgg cgcgccagcg tcagagaggc atcgagaaac tggttgcggt
     1681 cgagatcggc cacattaacc gtcgctttat aaattgtcgc tttaagcgcc ataagaaaac
     1741 tcctttcgaa tcaggaggcc attgtaacgg aatacgtgca gttgcggcgc tgatattgca
     1801 gaaatgagtg tggtatagtc accttgcgaa acagagttta tcttcgtagg cttagacttg
     1861 catccacggt taagtcagag tgctgacagg agggcatgtg gaaaaatatt gtgagttaat
     1921 acgcaagcgg tacgcggaaa tcgccagcgg agacttagga tacgttccgg acgcgctggg
     1981 ctgcgttttg aaagtgctga atgaaatggc tgccgacgac gccctttctg aggctgtcag
     2041 ggaaaaagca gcatacgctg cggcaaactt actggtgagc gattatgtca atgaatgata
     2101 cgtatcaacc aatcaattgt gatgattacg ataatcttga gctcgcctgc cagcatcatt
     2161 taatgctgac acttgagctg aaagatggcg aaaaattgca ggcaaaagcc agcgatttag
     2221 tctcccgcaa aaatgtggag tatctggtcg tcgaggccgc tggcgaaacc cgtgagctac
     2281 gtctggataa aattaccagc tttagccacc cggaaatcgg tacggtggtg gtgagcgagt
     2341 cctgattgac tttcgccgga tgcgacgttt gatgcgtctt atccggcttt cacttaacta
     2401 agcgttttct gccagacaaa acttacgcca ttctcacctt cagccacacc ttcttgcgtc
     2461 acaaataccc ctgccgccgc aatcagcgtt tcgccataaa acagcagtgg cgtggtgtca
     2521 cgtagccacg gcggcacgcc taactcttgc cagattttct tcagcttacg tccgccgttg
     2581 cgcccgacaa tatgcagcaa tcctgccgct ttgaaacgca cgctgaccgc ttcgtctgca
     2641 cgcggagggc gaatatcgcc tcccgcagta agctgtacgc ttcccagccc cgccggtaat
     2701 tccagcggtt gtagccacgt ctgccacggc acaatggttt cactttgccc ggtgacggat
     2761 ttaatccacc acagttgcga ctgatatcgg cggatttcaa acgcgcccaa gcgtaaacag
     2821 ggggaggcat cttcccgcgc cagcgccact tcctgccaga tccgcaccag cgcgtcgcgg
     2881 gaaggcatcg gtgcattctg ccctgccagc cagcggcgga taatcgccgc gcgacgggca
     2941 tcactcatcg ctagcattgg cgcaatctgc agcgtcccct gcgacgtttg gcagtgtgct
     3001 aaatcatccg ccagcagttc atccaacaga ctctcttgtt cagcacaaag tgcggcgctg
     3061 cgggccgttg cttcggcaaa atgcggccaa cgctgctgca ataacggcac tacacgcagg
     3121 cgcagaaagt tacgatcgta tgagtcgtcc tgattacttt cgtcttcaat ccagcgtaaa
     3181 ccatgcgcca atgcccactg cgccagttcc ccccgcgtgc gggcaagcaa cgggcggata
     3241 agccgtgttc cggcaaactc cgagacttcc cccatagccg aaagcccggc agggccgcta
     3301 ccgcgtttta gcgccagcag aaaggtttca cattgatcgt cgagatgttg cgcggtgacc
     3361 agcacttcgc cgggcaatag ggtacgggca aatgcctgat aacgcgcctg ccgcgcttgg
     3421 gcctcaatcc ccagcccctc ttgcgcaagt tgtacgcgtt cgaccaccag cggcacctgc
     3481 cactgttggc agacgttttc gcaatgcgta acccaggcat cggcattggc acttaaaccg
     3541 tgatgcacat gaatagcgcg cagagtgacg cccggatttt ccgtccgcca ctgcaccaac
     3601 tgatgcagca gaacggtgga gtcaagcccg ccgctaaagg ccaccagaat ctggcgtgag
     3661 gtgagaagtt gtctattgag cgtgagtgtc atgatggtgc gattttacag tagcaatgcc
     3721 cggcacgtta ccgggcaaat gcggcgatga caagccttac tgctcataca gttccaacgg
     3781 cagcccgtcc ggatcgttaa agaaggtgaa gcgtttttgc gtgtatggat cgacacggat
     3841 ggcttcacac ttcacgttat ggctttcaag gtgcgccact gccgcatcga tgtcatcaac
     3901 gctaaaagcc agatgacgca ggccgcaagc ttccggtcgg ctggggcgct ccggcgggaa
     3961 cggaaaagag aaaagctcaa tcacatattg cccattaagc gccaaatccc ctttccacga
     4021 gtcgcgcgct tcgcgataga cttcgctttg cagcgtgaaa ccaagaatat cgcagtagaa
     4081 agctttgctc accgcataat ccgtcgcaat aatcgcaata tggtgaacct gttttaaacc
     4141 cagcataacg tctcctttat ttgttaacag cacgttactc gcccggaagc cgctctggca
     4201 agttatcccg ccatttttag gactcgtacg cggtaaacgc cgtcttcgtc ctgtttcgcg
     4261 ccgtgaatat ccgtttcaaa accggggtaa tgttgcccta cggaacaaag catcagtaga
     4321 aaatcgagta ccgtgcggct ctctttggtc agcatttctc ctggcatcaa cagcggtacg
     4381 cccggtggat aaggcaggat catatttgcc gatactctac cgaccagttg ttccagcgca
     4441 atggtttcta cttcaccttt aatctgtctc tgccatgcct gatgtggcgt catgatcatt
     4501 tccggcaaag tatcgaatgc ccgcaacatc aaaccgggaa gatcgtgttt acgaatcagc
     4561 ttatggatcc cttgcgccag atcctgaata cgcatattgc ggtagaaatc gggatcttca
     4621 gcatagagat cgggtagcat atttttgatc cgcaggttga gatcgtaaga gcgtttaaat
     4681 tccgtcaacc cacgcaataa tcccattgct ttggttttat cgataccaat actaaagaga
     4741 aacagcaggt tataagggcc ggttttctct actacgatcc cacgttcgtc gaggaatttt
     4801 gccaccagcg ccgccgggat cccctcctca ctcatattgc cctgttcgtc catccccggt
     4861 gtcaaaatag tgactttaac cggatcgaga aacatatgat cggcatccgc atcgttaaag
     4921 ccgtgccact gttcgcctgg cgcaacgggc cagcattcgg cttcatccac ctgcggcggt
     4981 tgccagatat cgaaaaacca accgtcagac tcttcccgca accgctggac ctctttgcga
     5041 aaatgcagcg cccgctccac cgaacggtta atcagccgtt tgcccggatt accacgcagc
     5101 atcgccgccg ccgtctcaac cgaagcaaca atgggataac tgggcgaggt ggtggtatgc
     5161 atcataaagg cttcgttaaa cgcctcttcg tcatactcgc ctttaatgtg gatcagcgaa
     5221 gcctgcgata acgccgccag cattttgtgg gtcgattgcg tttcgaagat cacttttccc
     5281 gcaacacgct cgccgctcat accacttttc ccctggtaga tcggatgaaa atgggtgtac
     5341 ggcacccagg cggaatcgaa gtggatcgac gggacatcca gcgtctgttt gatccagtcg
     5401 gtgttgtaga gcaagccatc ataggtggag ttggtaatca ccgcatgaac cggccattgt
     5461 gcctgcgtgg tggcagcgac tttctcttcg atgctgtcgc gagtaaattc acggcgcggg
     5521 atcccaccaa gaatccccaa cgcattacgc gtcggtttca gccagactgg cactacatcg
     5581 ttcatcatca acagatgcgc cagcgattta tgacaattgc ggtcgatcaa cagcgtactg
     5641 ccggatggcg cggcgtacat acccacaatt ttgttcgacg tcgatgttcc gttggtaacg
     5701 atataactct gttccgcgcc aaaagtccgc gcgatgtact cttccgcttc caagtgtggc
     5761 ccggtgtggt cgagcaacga accaagctcg gtgaccgaaa tagagacatc agccttgagg
     5821 gtattcccgc cgaaaaaatc ataaaacagg cagccaaccg ggcttttttg atatgcggtg
     5881 ccgcccatat gccccggcgt acaaaaggtg tacttccgct ctttaacgta ggtaaacaag
     5941 gctttcgtga acggcggtgt aatgttatca agatattcgt cggtgtactg acgcatacga
     6001 atggcgatat cttccgcctg ccccagcgca tattcaaaaa accagagcgc catccgcata
     6061 tcctgcacgc tgacatccat cgtcgagtgg gtgttgatga aggcataaag cgggagatat
     6121 tcattaagtt gattgatatc gctgcataaa tcgagactgt actcatccca gtcaaaaatc
     6181 acgccgcaaa ttcgagggtt atgttcgata aatttcagca aatcaacgct gttttgtggc
     6241 cagataatct gaaagccttg cgccaccagc gccgactcca gttctttgat gggctcatct
     6301 ttataaaaga cgccatgcgg tcccataatg gcaatgatgt tcatgtgttc ctcctggaaa
     6361 atccttcctt aatcatagcc tgctcaaacc gtggcgataa aaaagggcca ccgaagtgac
     6421 cctttttcag aacttttgcg aattacgcgt aaccgtagct catcaggcgc tgataacgac
     6481 gattttttaa atcttcagtg cttaacacat cgagatcggc cagatccgcc agcagttgcg
     6541 ctttcaacga tgccgccatc gcttccgggt tacggtgagc gccacccagc ggttccggga
     6601 tgatggagtc gatcagtttc agctctttca gacgcggagc gatgataccc atcgcttcag
     6661 ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga cgcacaacct tccggcgaga
     6721 taacggaata ggtgctgtat tgcagcatat tcactttatc gcccacgcca atcgccagcg
     6781 caccgccaga accaccttca ccgataaccg tacaaactac cggtacgccg aggcgagaca
     6841 tttcacgcag gttgcgtgca atggcttcag actgaccacg ttcttctgcg cccacgcccg
     6901 gataagcccc cggggtgtcg ataaaggtga tgattggcat cttaaagcgt tcagccattt
     6961 gcatcagacg cagtgctttg cggtaacctt ctggcgctgg cataccaaag ttacggcgaa
     7021 ttttttcttt ggtttcacga cctttttgat gaccaatgat catcaccgga cgaccatcga
     7081 gacgggcgat accaccgacg atagctttat cgtctgcata cgcgcggtcg ccagccagtt
     7141 cgtcaaattc atcaaatgcc aggcgaacgt aatccagggt ataaggacgc tgtggatggc
     7201 gtgccagttg cgcaatctgc catgcaccaa gatcggcgaa gattttacgc gtcagttcta
     7261 cgcttttttc acgcagacga tgcacttctt catcgatgtt aatatccagt ttctcatcct
     7321 gacggctaac cgcagtcaga gaatcgattt tcgcttccag ctctgcaatc ggctgttcaa
     7381 aatcaaggaa attcagactc atagtattcc tgtattagtc aaactccagt tccacctgct
     7441 ccgaaccaat gaggccacgg agatcgttta ataaacgatc gctcggagag acacgccacg
     7501 tcgcgccaaa acgcaaccgc gcgcgtgcat ccgccctctg atagtagaga tgtactggaa
     7561 ttgtcccaga gcggtggggt tccagagact gacggagtcg gtttaaaagc tggtcatcaa
     7621 tttgcctgtc cgtcagcgag atagcaagcc cgcgagcata tttttcccgg gcttcgtcaa
     7681 tatccatcac ttcgcgagcg gtcattttaa gcccaccgct gaagtcatca aagctgacct
     7741 gtccgctgac gataagtatg cggtcttttt ccagcaattg ctggtattta tccagggcgt
     7801 cagtaaacaa catcacttcc agccgcccgg aacggtcatc cagcgtgcag ataccgatac
     7861 gattgccgcg cttggtgacc ataacccgcg cggcaacaac gagccccgca gccgtgatga
     7921 ctttaccacg ttctgtcggg tgcatgtctt tcagccttac gcctccgaca taacgctcaa
     7981 tctcttttaa atactggttg atagggtgtc cggtcaggta caggcctaac gtttcacgtt
     8041 ccccatctaa taccacctgc tccggccacg gttggcagct ggcgtaggat tgttcaattt
     8101 gttccggctc ttcggccagc acgccgaaca tatcggcctg accgatagct tccgctttcg
     8161 cgtgttgatc tgccgctttt aacgcatcac ccagcgagtt catcagcgcc gcgcggtgtg
     8221 gcccaagacg gtcaaacgcc ccggacatga tcagtttttc cagcacccgc cggtttaact
     8281 ttttggtgtc ggtacgggcg cagagatcaa acagttcgcg gaagtagccg cctttattac
     8341 gggcttcgat gatggcctca atcggacctt cacctacccc tttgatggcg ccaataccat
     8401 acacgatttc gccgtcgtcg ttgacgtgga aatggtaaag accggagttt atatctggtg
     8461 gcaggatttt cagccccatc cgccagcact catccaccag gcccaccacc ttctcggtgt
     8521 tgtccatatc ggcggtcatt accgccgcca taaactccgc cggatagtgc gctttcagcc
     8581 ataacgtttg atatgacacc aaagcatagg ccgcagagtg cgatttgtta aatccgtaac
     8641 ccgcgaattt ctccaccagg tcgaagattt tcatcgccag ttcggcgttg attccgttct
     8701 tttctgcacc ttcagcaaat acagaacgct gcttagccat ctcttccggc tttttcttac
     8761 ccatcgcacg acgcagcata tccgcgccac cgagggtata accagaaagc acctgcgcaa
     8821 tctgcatgac ctgttcctga tacaggatga tgccgtaggt tggctccagt accggtttca
     8881 ggctttcatg ctgccactgt acgtccggat aggagatttc ttcgcgacca tgtttacggt
     8941 cgataaagtt atccaccatc cctgattgca acggacctgg gcggaacagc gccaccagtg
     9001 cgatcatatc ttcgaagcag tcaggttgca gacgcttgat caggtccttc atgccgcgcg
     9061 attcaagctg gaataccgcc gtggtttccg agcgttgcag catgtcgaag cttttcttgt
     9121 catccagcgg gatcgcggcg atatccagcg gcggctcgcc attcttcgcc cgccgcttgt
     9181 tgatcatctc cagcgcccag ttgatgatgg tgagcgtacg caaaccaagg aagtcgaact
     9241 tcaccagtcc ggcgtattca acgtcgcttt tatcaaactg ggtgaccgga tgtttgccct
     9301 cttcatcgca gtaaagcggc gcaaaatcgg taattttggt cggcgcgata accaccccac
     9361 cggcgtgctt accggcgtta cgggtgaccc cttccagttt gcgcgccatg tcgatcagcg
     9421 ccttaacttc ttcatccgct tcgtagattt ccggcagctg cggctcggct tcaaacgctt
     9481 tcgccagcgt catccccgga tcgggcggga tcagtttcga gatacgatcg acaaagccgt
     9541 acggatgccc cagcacgcgg cctacgtcgc ggatcaccgc tttcgccgcc attgtaccga
     9601 aggtgatgat ctgcgatacc gcatcacgac cgtacatgtc cgccacgtgt tcgataacct
     9661 gatcgcgttt ctccatacag aagtcaacgt cgaagtcagg catggagaca cgttccgggt
     9721 taaggaaacg ttcgaacagc aggtcaaatt ccagcggatc gaggtcggtg attttcagcg
     9781 cgtaggccac cagtgaaccc gcaccggagc cacggcctgg ccctaccggt acgccgttat
     9841 ctttcgacca ctggataaat tccataacga tgaggaagta gcccgggaag cccatctggt
     9901 tgataacctg aagttcagtc tccagacgtt cgtcatattc cgggcggcgc ttaagacgtt
     9961 cttcctcatc agggaataaa aaggccagac gctcttccag gccctctttt gcacgcttga
    10021 ccagataatc ttcggtgctc atgtccccgg tcgggaactg cggcaggaag tattcaccaa
    10081 gacgcacggt tacgttacag cgtttggcga tctcaacggt gttggcaagg gcttcgggga
    10141 tgtcggcaaa cagctcacac atctcctctt cgctacgcat atattgctgc ggcgaatagt
    10201 tacgcgggcg tttaggatcg tcgagggtaa agccgtcgtg gatcgcgacg cggatttcgt
    10261 gtgcgtcaaa gtcgctgctg tcgataaagc gcacgtcgtt ggtcgccacg acgggcaaac
    10321 cgcgcgcttc cgccagttcc accgccgcgt gcagatagct ttcttcgtcc ggcctgccgg
    10381 tgcggatcag ctcgagaaaa tagcgatccg ggaagtgttc ttcataaaac gcgacacact
    10441 catctaccag cgcgctgtta ccacgcaaaa gactgcgtcc gacgtcgccc atgcgtccgc
    10501 cggaaagaag gatcaaccct tcgtttaatt cgataagcca gtcgcgatcg atgatcggcc
    10561 cggcggcacc gtacccgcgc tgatacgctt ttgagatcag caacgtcaga ttctgatagc
    10621 cggtattgtt cgccgccagt accgtcaggt gggttaactc atcacccagc aggtcgcact
    10681 ggacgttaaa atctgccccg acgataggct taatccctgc gccatgtccc gctccgtaga
    10741 acttcaccag accgcaaagg ttggtgaaat cggtgatcgc cagtgctggc atacccaacg
    10801 ccgccgcctt ttttaccagc ggtgcggttt tggccaggcc atcgatcatc gagtagtcgc
    10861 tgtgcacccg caggtgtacg aaacgtggtt cagacatctt cagattccgg tttacttaat
    10921 ctcgacacaa gaatcaggac gcaagtccca gtgcgcgttt gacaggccca aagctgcgcc
    10981 gatggtgttc ggtcgcgccg tgttcagcca gtttttccag atgaaaagcg gttgggtacc
    11041 ctttgtgttg ggcaaaacca tattgcggga aaacaatatc cagcgccgcc atttcggcgt
    11101 cacgcgtcac tttcgccagg atagacgcgg cactgatttc cggtacgcgg ctatcgcctt
    11161 tcaccacagc catcgcaggc atcggtaatt tcgggcagcg gttaccatca atcaacacat
    11221 attccggcgc aatatgcagc ccagcgacgg cacgctgcat cgccagcatg gtcgcatgaa
    11281 gaatgttcag ctcgtcgatt tcgtggggtt ccgcgcggcc cagactccag ctcaacgctt
    11341 tctctttgat ctcttcatag agcgccagac ggcgtttttc gctcagcttt ttggaatcat
    11401 tcagcccggc aatcgggcgc gccgggtcaa ggatcaccgc agcggtgacg accgcgccaa
    11461 ctaacggccc gcgtccgact tcatccacac ccgcaaccag ctgcgtgtgc ggataaacaa
    11521 attcgatcat tgtgctaact ccagaacggc ttgtgccgcc tgctcatcgg cattgcagcg
    11581 gatctgctga tgcagttcac ggaaggtatc gtgcatcgcg tggctggttt tcccgttcgc
    11641 caacagcggt aacagcgccg cagccagttt ttgcggctca cactcttcct gcaataattc
    11701 tttgactaac tctctgcccg ccagcagatt tggcagcgag acataatcag ttttcaccag
    11761 ccgcttcgcc aaccagaagg taaaaggctt catgcgatat cccaccacca tcgggcattt
    11821 cgccagcata cactccaggg ctgccgtacc cgacgccagt agcgccgcat cgctggcgac
    11881 catcgcctca cggcccatcc catccagcaa atgaactgaa aggtctggcg cgacttcagc
    11941 tttgatgcgt tcaaactgct cgcggcgttt ggcattcacc agtggcacca cgatttcgag
    12001 atccggatat gtctggcgca aaagctgggc cgttttcagg aaatcggcac taagcatttc
    12061 aacttctgcg ccacggctcc ccggtagcaa cgccaggcag tgggcatcgt gagggatccc
    12121 cagcacatca cgggcggcat ttttatctgg atctaacggc atggcatcag ccatggtatg
    12181 accgataaag cggcacggta cattgtattt gtcataaaac gctttttcga aaggcagaaa
    12241 tgcgagcacc agatcggtgg ctctgcctat tttgaaaacg cgtttctgtc gccacgccca
    12301 gacggatgga ctgacgtaat gaatggtttt gataccctgc tttttgaggt taccttcaag
    12361 agtaatattg aagtcgggcg catcaatacc aacaaaaaca tctggcttca gttcgccaaa
    12421 acgctttgtc agatcggcac gaatatgcag taagcgacgc agacgaccga gcacttcaac
    12481 aatgcccatt accgccagtt cttccatttc gtaccaggct tcgcagcctt cagcctgcat
    12541 tcgtggcccg gcaacaccaa caaagcgggc gttgggcaca cgttctttca gagcgcggat
    12601 taaaccggcc cccaggatat cgccggaggt ttctccggcg accagggcaa tcgttaatgg
    12661 acgctgttca gtcattaacg aatcagaccg cgcgttgagc gtgcaaagaa atcggtaaag
    12721 gctttcactt ccggatatgt ttccgccagt tcagcaattt ccggtttcac ttcatcgagc
    12781 gttttaccgc tacgataaat cagcttatac gcattgcgga tagcggtaat cgcctcacgg
    12841 ctgaatccgc ggcgcttcag cccttcgata ttgacaccga acggcgttgc gtggttaccc
    12901 tgcgcaatga cataaggagg gacgtcctgc gccacaccgg agcagccgcc aaccatcacg
    12961 tgcgcaccaa tgatgcagaa ctgatggact gcggtcatgc cgccgatgat cgcgaagtcg
    13021 tcaaccgata cgtgacccgc cagcgttgcg ttgttggcga gaatacagcg gttacctacc
    13081 gtacaatcgt gcgcaatgtg cgcgttgatc atcagtaagt tgtcgctgcc caccttcgtc
    13141 aatccaccgc cctggactgt gccacgatga atggtgacgc tttcgcgaat gcggttacga
    13201 tcgccgattt ccacacgggt cggttcgcca gcatatttca gatcctggtt aacttcgccg
    13261 atggaggcga actgataaat ctcattatcg cggccaattt tagtatgacc attcacgaca
    13321 acgtgagatt tcagtacggt accctcacca atttcgacat ggggtccaac gatacaaaaa
    13381 ggaccaatgt gtgcgttcgc gccaattgac gcgccctctt ccacaatggc ggttggatgc
    13441 acaaaggcgg atttatcaat cacgtatcag gcctcccggc tacgagcaca catcatcgtt
    13501 gcttcgcaaa ctactttacc atcgaccaga gcaacccctt taaaacgggt caggccgcgg
    13561 cgcgtttttt cgaaagtgac ttccatgatc atttgatcgc caggcacgac cgggcgcttg
    13621 aagcgcgctt cgtcaatacc agcgaagtag tacagctcac ccggttccag ttttcctacg
    13681 cttttaaacg ccagaatacc tgttgcctgt gccattgctt ccagaatcag cacacccggg
    13741 aaaatcggtt ttccagggaa atggccctgg aagaatggct cattgacaga gacatttttt
    13801 actgcgcgca gaaaacgacc ttcttcaaaa tccagcacgc gatccaccag taagaacggg
    13861 aaacggtgcg gcagaagttc taaaatctct tcaatctgca gagtatgagt gttagtagtc
    13921 aagatactct tcctgtcaaa atataagaaa cgacaataat aacacggcct gccgcaatcg
    13981 taagaatgag acaggccgta aagtttggcg aacaaaagat ggaacgttag tcttgttgat
    14041 taaccttgcg ctcaagcgat ttcagacgct tgctcatgtc atcaatgttc atcaccagtg
    14101 cagcggtttt gcgccagact ttgttgggtt gcagcggaat gcctgaggaa tagacgcctg
    14161 gttcagtgat gggacgcatc accataccca tgcccgtaac cgtcactttg tcgcatattt
    14221 ccatatgccc gttgattacg ctggctccgc cgatcatgca gtaacgacca attttcaggc
    14281 tgcccgccat aatgacgcca ccggcaaccg ccgtattgtc gccaatcacg acgttatgtg
    14341 caatctggca ctggttatca atgatcacgc cattgccaat aatagtgtca tccagcgcgc
    14401 cgcgatcgat ggttgtgcag gcaccgatct ccacgcgatc gccaataatt acgcgaccaa
    14461 tctgtgggat cttcacccag ttaccacgat cgttggcata accaaagccg tctgcgccta
    14521 ccactgttcc ggactggatc aggcaattct gaccgatctg gatctcatgg taaatggtta
    14581 cgttcgccca gagacgcgaa cctgcaccga ttttgctgtt attacctacg aagcaaccgg
    14641 caccgataat cacgttatcg cccagttcaa cgccggactc aatcaccgcg ttagcgccaa
    14701 tcgatacgtt gttacccagc ttcgccgtcg cgtcgatcac cgcactgggt gcaatgttct
    14761 gcgcgggctg cggcgtggta tctaaaattt gcgccatgcg cgcgtaagtc aggtagggat
    14821 tcttcactac cagcgcggca cttttcgcga aaggaagatc gtcctgggtc atgacaaccg
    14881 cggacgcctg gcacaagcct aaatgctcac ggtattttgg gttaaccatg aacgtaatgt
    14941 gacctgtttg tgcagattgc atggacgcaa cgccggtgat gacgatatcg ccatcaccgt
    15001 gtagttctgc atccaactgc tgcgctaaat cagccagtcg aattgaaggc attacttatt
    15061 taacctgttt cagtacgtcg gcagtgatgt cttttacatc gctgctgttg taagcaacgg
    15121 cgtttgcatc aacaaccaga tcgatatcct ggctgttggc aacggatttc acagcagtct
    15181 ggatacgagt aaccagtttg ccgcgttctt cgttggaacg acgtgcgcga tcctgctcaa
    15241 aagcctgcgc tttctgagca aaagtctggc gctgagccat cacgtctttt tccagcttag
    15301 tgcgatcgct gcccgctttc atggactgca gctttttcat tttagcctgc agatcggttt
    15361 ccatacgctg cagttcgctg gcacggcctt tgaactcatt ttccagcgtg ttagaaacac
    15421 cggttttctg cgctacctgc tggaacaggc tgcccatgtt gacgattgca attttgtcag
    15481 ccgcctgagc agaagttgcc agtgctaaac cgagacctgc agctaataac cactttttca
    15541 caataaactc cttaccatcc catttgcacc ggagggtgca gttctttgcg tggcccggcg
    15601 atcttatatt gatcgcctaa agtcatcgct acactaccac tacattcctt tgtggagaac
    15661 acttaccagg ttttaccgat gttaaactgg aactgttctg ccttgtctcc atcgtacttt
    15721 ttgaacggct gggcgtagga gaacaccaac ggccccaatg gggacatcca ttgtaatgcg
    15781 atacccgcag acatacggat attgcttgga tcactatagt ccggatatcc agaatattgg
    15841 ctggaatccc agtttgtatc ccaaacggta cccatatccc agaagaagga agtacgaacc
    15901 gagttagcat acttatcgct aataaacggc gtcggggtga tgaactcgag gctggcaacc
    15961 gccatggcgt taccgcctac agcatcatcc gatttacaca ggtctttcgc gccgtcctga
    16021 gtcgcacatt cgtaatcata gtccggatca taattactgg cctgatgcgg gaagtaaact
    16081 gctttcggac caattgtatt ggactggaag ccacgcacgg tgctggaacc accggcatag
    16141 aagttctcgt agaacggcat ctctttgccg cctaaaccat caccataacc ccagcgggta
    16201 cgccccagaa caacccattt gtgatcgtca tcgatcggca cataagtcgc cgtgtctaac
    16261 gtcactttgt agtattcgtt atccgatcca ggaatggtca ctttaccggt caggttgaca
    16321 cgtgaaccat ctgtcgggaa gtaaccacgg tcaagcttgt tataggtcca accatagttg
    16381 aacgtgaagt cgtccgtttt gaagctatta tcctgatcag aggtgctcgg atgttcaccc
    16441 atagagtaca gataacgcca catcgcaacc tgaggctgca tgttggacag ggagttatgt
    16501 acataaccca gacctgcacg cagcgagtta tattcgttga tcgggaagcc caacgtcacg
    16561 tctgtaccat aactcttgtt ggtatagtcg gacaggtcgg cgtcatctgc ctggaagtca
    16621 ttatagaaga gacgaccacc gaggcttaca ccatctacag tgaagtacgg gttggttacc
    16681 gacagttcag cataggtctg gtaatcgttt ttggtcccgt tgataccaac agcataacct
    16741 gtacctaacc agttatcctg ctgcacacca gcctggaagc tcacgccact ttcagtaccg
    16801 taaccaatac caaagttgaa gctaccggtg ttgcgctctt ttaccttgta gacgacatct
    16861 acctggtccg ggctacccgg aacacgttgg gtatcggtat cgacagtttc aaagaagccc
    16921 agacgattca gacgctcctt accctgatcg accagatcgc ttcccagcca tgcaccttcc
    16981 atctgacgca tttcgcgacg caggacggca tctttcgagg tatcgttacc ttcaaaacgg
    17041 atcttacgca cgtagaaacg gttacccgca tcaacgttca cgcgtaattt aacggttttg
    17101 tcggcatcgt taatttcagg catcgactgt acgcgcggat aggcataacc atagcgaccg
    17161 agaagctttt tgatgtcatc ttccatcttg gtcactttgg tgccgttata cagctcaccc
    17221 ggctcgatct tagtcagctg ctcaatttca gcggagtgcc cggcaaggtt gccgctcact
    17281 tcaacgccag aaagcttgta ctgatcgcct tcggtgatgt tcaccgtgac gtaaatacct
    17341 tttttatctg gcgtcagact gacctgggta gagtcgatgt tgaaacgggc ataaccgcga
    17401 tccagatagt agctgcgcag ggtttcaagg tcgcccgcca gtttctgttt ctggtattta
    17461 cgatcgccta ccacgttcca ccacggcact tcgtcacgca gttggaaatg agagatcagt
    17521 tcgtcggtgg tgaaagcatg gttaccaaca atgttaattt gctggatttc agctgacaca
    17581 ccttcctgga acaccagttt taggtcaaca cggttgcgcg gcagcggggt cacgacagct
    17641 tttacgctgg cgctatattt accgacgctg tagtagaagt cttccagacc tttctcgata
    17701 tcggcaatgg tggtgcgatc gagggattcg cccacacgca caccagaagc ctcgaggttt
    17761 tgcttcagca tgtcatcttt caccgatttg ttaccggaga aagtaatgct ggcaatggtc
    17821 ggacgttctt ttacctgaac cagaagggta tcaccatcac gaaggacgcg aacatcctca
    17881 aagttgccgg tagcaaacag agcgcgaatg gtattactga tatcttcatc attaaccgtg
    17941 tcgcctgtgc gcaccggcat actgaggagg gccgcaccaa cggcgacacg ctgaaggcct
    18001 tcgaaatgaa tatctttcac tacgaaccct tcagcaccgt atacggtggc gctgctaaac
    18061 agcagcgacg ctatgagcaa ctttttcatc gccatcgtta ttatgcgttc ttcctaacta
    18121 actctcataa ccgagagaaa tcattgaaaa gtgcaagccc cattaacagc accagcagaa
    18181 tcgagccaat gcgataacaa aagtcttgaa cccgctcgga taccggtccg cccttgatct
    18241 tttcgatcgc aaggaacagc agatgccccc cgtcaagtac gggcaacgga aacaggttaa
    18301 ttatccctaa gttcacgcta ataagcgcaa gaaacggcag gtaataaact accccgagtt
    18361 ccgctgtcat cccagccccc ttggcgatag agatcggccc actgaggttg ttcagtttca
    18421 catcaccggt gatcaatttt cccagcatac tgaccgtcag cttcatcagc tgccacgttt
    18481 tgtccgtggc ttcgacgatg gcgttgaacg gcccatactg gcgtacaact ttatactcat
    18541 ctggcaaagg aatgactttc ggctcaatac cgacaaaacc aatcgcttta ccattacccg
    18601 gtttactctc cgggattaat gtcaaagaca agggacttcc ctgcctttcg atttctaacg
    18661 ctaaggattt acccgggtta tcccggacaa gcatcacaaa ggtcacccac tgcgttaagg
    18721 gctgaccatc gactttaacg atcctgtcgc ctgcttgcaa acctgccttg cttgccgccg
    18781 agtttggctg cacattttcc agtacaggtt caatttgcgg cccacgagga cgaatcccca
    18841 gcgaagatac cggatcttct ttatcaggct caaacgccca gtgacgtaaa tcgagcttta
    18901 catcccgccg ttgatcgctg ccaaatggcg ctactgtaat ggtggtgctt tcatcgccaa
    18961 ttttatcgac caactgcaaa cgcacggcat cccaatcagg cgtttcgata ccatctacgg
    19021 cttttagttc cgtacctggt gcaatttgtg cttccgcagc tatcgaattg gctgctattt
    19081 caccaaccac cggacgtacg ccaggcacac caataataaa aaccagccag taggcaaaga
    19141 tagcaaaaat gaagtttgca accggacctg cggcaataat cgccgctcgt tggccaacag
    19201 atttattatt gaaggcatgg tggcggagtt ccggaacgac cggttctgcg cgctcatcca
    19261 gcattttgac ataaccgccc agcgggatca gggcgataac atattcggtg ccgagcttat
    19321 cagttcgccg ccagagcgcc ttaccaaacc ctattgagaa acgctcaacg cgaacaccac
    19381 aacgccgggc aacccagaaa tgaccaaatt catgcacggt gataagtaca cccagtgcaa
    19441 cgatgaacga agccaaatcc cagagaaaac tcagcatatt accttccgtt aaagcgtcct
    19501 gaataccagt aacaacaagc aagcaaagac cggtaccgca gccgtcaggc tatcaatacg
    19561 atctaaaata ccaccgtgtc ctggaattaa atgaccgctg tccttaattc ctgcttcacg
    19621 cttaaacata ctctcggtca gatcgccgag cactgaggct aacgctgcga caatagagca
    19681 aatgagtaag gtgacgggag cgacgtcgag attcgcccac atgccataac cccatgagat
    19741 taccgctgcg gtcgctagtc cacctataaa gccttgccag gttttacccg gagaaacctt
    19801 cggtgccagc ttatgtttac caaacaattt gccaaacata tatgcgccag agtcagcgcc
    19861 ccataccagg atcatgacat agagcagcca tattgcgcca ctgtaatgat tctcgtcgta
    19921 gtgccaggcc cgtaacgcca gcatgcccca gaagaaggga acaatggtta gcacgccaaa
    19981 aataaggcgc aatgttttag agttacgcca gattgctgcg gaacctgggt aaaacagcac
    20041 cagcaatagc gcgacaatcc accaacccag cgaagcccaa agtgagattt caaccagcgg
    20101 ttgatgaata tttcggtgat attccggcaa cagaaaaagc atcagcgcca acaataaccc
    20161 gcataacacc gccaaccata ctcgctgcga acgagtggta aaaccgctaa gctgtcccca
    20221 ttcccacgct gccagcatgc agaccaccag cgttacgatg gcgaacccca ccggcggcaa
    20281 cagaaacaac gccgcgatga cgacgggtat taacacaaaa gcagatatca ggcgatactt
    20341 cagcaaaagc gacccccatc aggctgtttc atcaccgggc tcggtgccgc cgaaacgacg
    20401 ctctcgatta gcaaaggcat ttaacgcccc ttcaaagtct tgttcatcga aatcgggcca
    20461 gagaacatct gtaaagtaaa gttcggcata ggcaatttgc caaagcaaaa agttactaat
    20521 gcgatgctcc cccccagtcc taattactaa atctacaggg gccagttcat gcatacagac
    20581 atgctggttt agcatctctt catctatctg atctggttgc aggtttcctt gctgcacctt
    20641 ttcagccagt tgtctgactc cctggactat atcccaacgt ccaccgtagt tcgccgcaat
    20701 attcagcgtc agaccggtat tcccggctgt tagcgcttca gatttacgaa tacgttcttg
    20761 caaacgcgag ttaaagcgac tggtatcgcc aataatacgc agacgcacgt tatgtcggtg
    20821 cagacttttt acttcgctat cgagcgccca cacaaacagt tccattaacg cactgacttc
    20881 ctgcgctggt cggttccagt tttcactact aaaggcatac agcgttaacg cctcaatacc
    20941 gttgttggcc gcaaaagaga cagcccggcg gacggatttt gccccggctt tatgcccaaa
    21001 ggcacgaatc ttcccttgct tttttgccca gcgaccattg ccgtccataa tgatcgcaac
    21061 atgacggcag ccatgcgctg gcaatttttc gctaagtggt tgagtagcag acaacatcac
    21121 gcgtttttta ttccctgaca gggtttacgg tactcaggaa tactgaagcc tatacaaaaa
    21181 aaagccgtgt aaaaccacgg catacctgac cttataaagc caactactta cgatcagatg
    21241 gcgcagacta tatcactgaa gccctacgct aacaaatagc gcgactctct gtagccggat
    21301 tatcctcagc ttgcgagacg catcacctct tttctggcga cttcacgcgc gttcgcatca
    21361 acagataaca catcgtccac acattgtggt tcgcgcatat ccattttttc cagtacggat
    21421 aaattcaacg cagcgatatc cgtaaagcgg atttgttgcg caagaaaagc agcaacggtg
    21481 atttcgtttg cggcattcaa tgctgtcgtc gctgcctgac cttgttcgaa cgcctccatc
    21541 gccagtttca ggcatggata acgatcataa tccggtgcgg caaatgtcaa cgcacttagt
    21601 ttgcaaaaat cgagcggctt cacgccagag ttcacgcgat tcggccatgc catggtgtgg
    21661 gcaattggcg tacgcatatc cggttccccc agctgcgcca gaacactgcc gtcctgatag
    21721 cgcaccattg agtgaatcac tgactgcggg tgaatcagca cttccatctg gctggcgctg
    21781 gcgttaaaca gccaacgcgc ttcaatgtat tccagacctt tgttcatcat ggtagccgaa
    21841 tcgacagaaa ttttacgccc catcgaccag ttcggatgac ggcaggcttg atccggcgtc
    21901 attgttgcca aatcgcgcaa tggcgtctca cggaaagggc caccagaccc ggtaagtaaa
    21961 atggacacca cgccattttg ctcaaggtca gcgtatccca gattatgctg gataggttgc
    22021 ggtaaactct gaaaaatggc gttatgttcg ctatcgaccg gtaacaattg cgctttgctc
    22081 tgctttacgg cgtccataaa cagacgtccg caggtaacca gtgattcttt attggccagc
    22141 aaaatggttt tacccgcgcg gatcgcagca agcgtaggta acagcccagc agcgccaaca
    22201 atggcggcca tcacctgatc aacatcctca agcgctgcca tatcgcaagc ggcttgttgc
    22261 ccacttaaga cttcggtgcg gctaccctgt tgctgtagca tcgttttaag aagtttcgca
    22321 ctcgcttcat cgtccattac ggcatagcgg ggagagaatt ccaggcactg ttctaccatg
    22381 cgagtgacat ttttgcctgc caccagcgca actacgcgga agtgttcggg attatggcgc
    22441 accacgtcca gcgtgctgca accaatcgag ccggtcgagc ccagaatggt gagttgcttc
    22501 atgaaacatc cagagttgag acagaataaa aagcaaaacg ccgccagccg atccgcaagg
    22561 atctactgag cggcgttttt gtcgttcaag aaatcagaac tgcatcagtt ctgcttcttt
    22621 gtctgccagc gccgcttcaa ttttcttgat tgcagcatca gtcagtttct gtacatcgtc
    22681 ctgagaacgg cgatcgtcgt cttcgctgat ctctttatct ttcaacagtg ctttcacttt
    22741 gtcgttcgcg tcacgacgca cgttacgtac tgcaacacgc gcttgttctg cttcaccacg
    22801 aacgattttg gtcagatctt tacgacgttc ttccgtcagc ggcggcagcg gaacacggat
    22861 gtcgctaccc gcagagttcg ggttcaggcc aagatcggac gccataatcg ctttttcaac
    22921 ggccggagac attgaacg
//
LOCUS       DAFATA010000071        22770 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFATA010000071 DAFATA010000000
VERSION     DAFATA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.072"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..297)
                     /locus_tag="LGJ20_003226"
     CDS             complement(<1..297)
                     /locus_tag="LGJ20_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387249.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMP"
     gene            complement(540..746)
                     /locus_tag="LGJ20_003227"
     CDS             complement(540..746)
                     /locus_tag="LGJ20_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBK2387250.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            1059..1148
                     /gene="kdpF"
                     /locus_tag="LGJ20_003228"
     CDS             1059..1148
                     /gene="kdpF"
                     /locus_tag="LGJ20_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBK2387251.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            1148..2821
                     /gene="kdpA"
                     /locus_tag="LGJ20_003229"
     CDS             1148..2821
                     /gene="kdpA"
                     /locus_tag="LGJ20_003229"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBK2387252.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2844..4892
                     /gene="kdpB"
                     /locus_tag="LGJ20_003230"
     CDS             2844..4892
                     /gene="kdpB"
                     /locus_tag="LGJ20_003230"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBK2387253.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVNAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4901..5473
                     /gene="kdpC"
                     /locus_tag="LGJ20_003231"
     CDS             4901..5473
                     /gene="kdpC"
                     /locus_tag="LGJ20_003231"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBK2387254.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5466..8150
                     /gene="kdpD"
                     /locus_tag="LGJ20_003232"
     CDS             5466..8150
                     /gene="kdpD"
                     /locus_tag="LGJ20_003232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBK2387255.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            8147..8824
                     /gene="kdpE"
                     /locus_tag="LGJ20_003233"
     CDS             8147..8824
                     /gene="kdpE"
                     /locus_tag="LGJ20_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBK2387256.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            9099..9203
                     /locus_tag="LGJ20_003234"
     CDS             9099..9203
                     /locus_tag="LGJ20_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBK2387257.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9514..11712
                     /gene="speF"
                     /locus_tag="LGJ20_003235"
     CDS             9514..11712
                     /gene="speF"
                     /locus_tag="LGJ20_003235"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBK2387258.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11709..13028
                     /gene="potE"
                     /locus_tag="LGJ20_003236"
     CDS             11709..13028
                     /gene="potE"
                     /locus_tag="LGJ20_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBK2387259.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(13084..14724)
                     /gene="pgm"
                     /locus_tag="LGJ20_003237"
     CDS             complement(13084..14724)
                     /gene="pgm"
                     /locus_tag="LGJ20_003237"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBK2387260.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14750..15295)
                     /gene="seqA"
                     /locus_tag="LGJ20_003238"
     CDS             complement(14750..15295)
                     /gene="seqA"
                     /locus_tag="LGJ20_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBK2387261.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15480..16244
                     /gene="ybfF"
                     /locus_tag="LGJ20_003239"
     CDS             15480..16244
                     /gene="ybfF"
                     /locus_tag="LGJ20_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK2387262.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16384..16677
                     /gene="ybfE"
                     /locus_tag="LGJ20_003240"
     CDS             16384..16677
                     /gene="ybfE"
                     /locus_tag="LGJ20_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBK2387263.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16817..17347
                     /gene="fldA"
                     /locus_tag="LGJ20_003241"
     CDS             16817..17347
                     /gene="fldA"
                     /locus_tag="LGJ20_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBK2387264.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17557..17643
                     /gene="uof"
                     /locus_tag="LGJ20_003242"
     CDS             17557..17643
                     /gene="uof"
                     /locus_tag="LGJ20_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBK2387265.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17636..18082
                     /gene="fur"
                     /locus_tag="LGJ20_003243"
     CDS             17636..18082
                     /gene="fur"
                     /locus_tag="LGJ20_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBK2387266.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18166..18492)
                     /locus_tag="LGJ20_003244"
     CDS             complement(18166..18492)
                     /locus_tag="LGJ20_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387267.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18542..19948)
                     /gene="chiP"
                     /locus_tag="LGJ20_003245"
     CDS             complement(18542..19948)
                     /gene="chiP"
                     /locus_tag="LGJ20_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBK2387268.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20394..22058)
                     /gene="glnS"
                     /locus_tag="LGJ20_003246"
     CDS             complement(20394..22058)
                     /gene="glnS"
                     /locus_tag="LGJ20_003246"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBK2387269.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22261..>22770)
                     /locus_tag="LGJ20_003247"
     CDS             complement(22261..>22770)
                     /locus_tag="LGJ20_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK2387270.1"
                     /translation="PATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASK
                     AVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEIVVHMGIDTVALEGKG
                     FKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAG
                     QTPLYEIKK"
BASE COUNT         5214 a         5750 c         6043 g         5763 t
ORIGIN      
        1 aggcattttc cagccggggc cgaggctccc caccggcgcg ggcgtttttg tccggtaact
       61 gctgtaggtg cgggagagga tgaacggcag cggggcgggc agggcgatgt cggtttcacc
      121 gggaaggacc tttgcaccga gcagcggatt gaccggatgg ccggacgtca ccccgccggg
      181 gcacaccgaa caggccacgc cggtgggggc accgatgcgc accccggctg aaccctgaac
      241 aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgcc gccggttttc cgctcatctg
      301 ctcatccttg ttttatcggg ttatttgtct gctgacaggc gaaaccctac agatttataa
      361 gaaagcctta agtgagatat ctgtcagata agaacaatta ggaaaatatt tatatatatt
      421 tcatgaatat atgtttcgat tggtataacg atgtctaaat attcctctga acatcttcct
      481 gatacaaaaa accgccagcg tttccgctgg cggttttcaa tgtattcaaa actcagcaat
      541 caataaaaat caccagttgc cttttcggcc tgatccatcc acaccggttt atcgctggtt
      601 ttcgaccaga cgcgatgcag gtagctgtaa aagcgggcgc ggtctttcca gaaaagcatg
      661 aaaggcagcg ccagaacgcc cgctgcaacc gcataagtac ggcgtaaaaa gataagccat
      721 gcaggatatt ctctgtagag ttccatactt ttctccccct tttgtatcta cccggtgaat
      781 ggcaccggaa aaatgaattt gtttatctga tgaaaatagt accgcctttt gtgtaatttt
      841 actactcatc cgaccactta tttttgctta ttgatggttt atttacattc atcctgtaat
      901 taagttacac aaaagttaaa ttaatactaa acattagtta aatcatggct tttgccattt
      961 ttatactttt tttacacccc gcccgcagat ttttgcgaaa tctttgcagc cagaattcta
     1021 cccttccggt atcactttta ggccactgga ggtgcactgt gagtgcaggc gtgataaccg
     1081 gcgtattgct ggtgttttta ttactgggtt atctggttta tgccctgatc aatgcggagg
     1141 cgttctgatg gctgcgcaag ggttcttact gatcgccacg tttttactgg tgttaatggt
     1201 gctggcgcgt cctttaggca gcgggctggc gcggctgatt aatgacattc ctcttcccgg
     1261 tacaacgggc gttgagcgcg tactttttcg cgcacttggc gtctctgacc gtgagatgaa
     1321 ctggaagcaa tatctttgtg ccattctcgg cctgaacatg ctggggctgg cggtgctgtt
     1381 ttttatgttg ctcggtcagc actatctgcc gcttaatcca cagcagttgc cggggctgtc
     1441 gtgggatctg gcgctgaata ccgccgtcag ctttgtcacg aataccaact ggcaatctta
     1501 cagcggtgaa accacgttga gctatttcag ccagatggct ggcttaacgg tgcaaaactt
     1561 tctttctgcc gccagcggga ttgcggtgat ttttgccctc atccgtgcgt ttacccgcca
     1621 gagcatgagc acactcggga atgcctgggt cgatctgcta cgcatcacgt tatgggtgct
     1681 cgtccctgtg gcgttgttga tcgcactgtt ttttattcaa caaggtgcgc tgcaaaactt
     1741 tcagccttat caggctgtga ataccgttga aggagcgcaa cagctgttac ccatggggcc
     1801 tgtagcttct caggaagcga tcaagatgct cggtactaac ggcggtggct tctttaatgc
     1861 caactcgtcg catccgtttg aaaacccaac cgcactgacc aacttcgtgc agatgctggc
     1921 gatcttcttg atcccaacgg cgctgtgctt tgcctttggt gaagtgacgg gcgatcgccg
     1981 ccaggggcgc atgttgctgt gggcgatgtc agtgattttt gtcatctgcg taggcgtggt
     2041 gatgtgggca gaagttcagg gtaatcctca tctgctggcg ctcggcgcag acagcagcat
     2101 caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc agtagcctgt ttgcggtcgt
     2161 gactacggcg gcttcctgtg gcgcggtgat tgcgatgcat gattcgttta ccgctctcgg
     2221 tggcatggtg ccgatgtggc tgatgcaaat tggtgaagtg gtgttcggcg gtgtcggttc
     2281 tggtctttac ggcatgatgc tgtttgtcct gctggcggtg tttattgccg ggctgatgat
     2341 tggtcgtaca ccggaatatc tgggtaaaaa aatcgacgta cgcgagatga aactgactgc
     2401 actggcgatt ctggttaccc cgacgctggt gctgatgggc gcggcgctgg caatgatgac
     2461 cgacgccgga cgtagcgcca tgctcaaccc tggcccgcat ggttttagcg aagtgctgta
     2521 cgccgtgtca tccgccgcta acaacaacgg cagcgccttt gccggattaa gcgccaactc
     2581 cccgttctgg aactgtttac tggcgttctg catgtttgtc ggtcgcttcg gggtgattat
     2641 cccggtgatg gcaattgccg gttcgctggt gagtaaaaag agccaacccg ccagctccgg
     2701 cacgctgcca acgcacggcc cgctgtttgt tggcctgtta atcggcaccg tgttgctggt
     2761 tggcgcactg acctttatcc ctgccctggc gcttggtccg gtggcggaat atctctcctg
     2821 atgatattga gtgagcactg aatatgagtc gtaaacaact ggcgctattc gaaccaacac
     2881 ttgtcgttca ggcgctgaaa gaagcggtga aaaaattaaa cccgcaggcg caatggcgca
     2941 atccggtgat gtttatcgtc tggatcggca gtctgctgac cacctgtatt agcatcgcga
     3001 tggcaagcgg tgcgatgcct ggcaatgcgc tgtttagcgc ggcaattagc ggctggctgt
     3061 gggtcaccgt actgttcgct aatttcgccg aggcgctggc agaaggccgc agtaaagcgc
     3121 aggccaacag tcttaaaggg gtgaaaaaaa ctgcctttgc ccgcaagctg cgtgagccga
     3181 aatatggcgc tgcggcggac aaagttcctg ccgatcaact gcgtaaaggt gacatcgtgc
     3241 tggtagaagc tggcgatatt atcccctgcg atggtgaagt cattgaaggc ggcgcatcgg
     3301 tcgatgaaag cgccatcacc ggggaatcgg caccggtgat ccgtgaatcc ggcggcgatt
     3361 ttgcctccgt caccggcggc acgcgtattc tttctgactg gctggtgatt gagtgtagcg
     3421 ttaaccccgg tgagacattt ctggatcgga tgatcgcgat ggtggaaggc gcacagcgac
     3481 gcaaaacacc gaacgagatt gccctgacta tcctgctgat tgctctgacc atcgtctttt
     3541 tactggcaac cgccacgctg tggccgtttt ccgcgtgggg cggtaatgcc gtgagtgtaa
     3601 cggtactggt ggcgctgctg gtctgtctga tcccaaccac tattggcggc ctgttgtcag
     3661 cgatcggcgt cgccgggatg agtcggatgc tgggcgcgaa tgtgattgcc accagcggac
     3721 gtgcagttga agcggcaggt gacgttgacg ttctgctact ggataaaacc ggcaccatca
     3781 cactcggtaa ccgtcaggcg tcggagttta tccctgcgca gggcgtggat gaaaaaacgc
     3841 tggctgacgc cgcacaactg gcttcgctgg ctgatgaaac gccggaaggc cgcagtattg
     3901 tgatcctcgc caagcaacgt tttaacctgc gcgagcgcga tgtgcagtcg ctccatgcca
     3961 cctttgtacc gtttactgcg caaagccgga tgagcgggat caacatcgac aaccgcatga
     4021 tccgtaaagg ttctgtcaat gccattcgcc gccatgttga ggctaacggc ggtcacttcc
     4081 ctaccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca gggggccacg ccgctggtgg
     4141 tggtggaagg ttcccgtgtg ctgggcgtta ttgcgctgaa agatatcgtc aaaggcggta
     4201 ttaaagagcg cttcgcccag ctgcgcaaaa tgggcattaa aacggtgatg attaccggcg
     4261 ataaccgtct gactgccgcc gccattgctg cggaagcggg cgtcgatgat tttctcgccg
     4321 aagcgacacc ggaggccaag ctggcattga ttcgtcagta tcaggcggaa ggccgtttgg
     4381 tggcgatgac cggcgacggc accaacgatg ctccagcgct ggcgcaggca gatgtcgcgg
     4441 tggcgatgaa ctccggtact caggcggcga aagaagcggg caatatggtc gatctcgact
     4501 ctaacccgac caagttgatc gaggtggtac atattggcaa acagatgctg atgacccgtg
     4561 gctcgctgac caccttcagc attgccaacg atgtggcgaa atacttcgcc attattccgg
     4621 cggcgttcgc ggcaacgtat ccgcagttaa atgcgctgaa catcatgcgc cttcattcgc
     4681 ccgactccgc cattctcagt gcggtcattt tcaacgcctt gattatcgtc tttttgattc
     4741 ctctggcgtt aaaaggcgtg agttataaac cgcttaccgc ttctgccatg ttgcgccgta
     4801 acttatggat ttacggtctg ggtgggctgc tggtgccgtt tattggtatc aaagtcattg
     4861 atttactgct gaccgtttgc ggtctggtgt gaggtttacc atgcgtggct tacgtccggc
     4921 attatcgaca tttatctttc tgttattgat tactggcggc gtttacccgc tgctgaccac
     4981 cgtgctgggg caatggtggt ttccctggca ggccaacggt tcgttgattc gtgaaggtga
     5041 tacggtgcgg ggttcggcat taatcgggca gaattttacc ggcaacggct attttcaggg
     5101 tcgcccgtcg gcaacggcag aaatgcccta taatccacag gcttctggcg ggagcaatct
     5161 ggcggtcagt aaccctgagc tggataaaca aatagccgca cgcgttgctg cattacgggc
     5221 cgctaacccg gatgccagta cgaacgttcc ggttgaactg gtgacggcat cggcaagcgg
     5281 gctggacaat aatatcaccc cgcaagcggc ggtctggcaa atcccacgcg tggcgaaagc
     5341 gcgtaatctc agcgttgaac agctcacgca actgatcgca aaatacagcc aacaaccgct
     5401 ggtgaaatat atcggccagc cggtcgtcaa cattgttgaa ctcaatctgg cgctggataa
     5461 acttgatgaa taacgaaccc ttacgtcccg accccgatcg tctgctggaa caaactgccg
     5521 cgccgcatcg ggggaagctg aaagttttct tcggtgcctg tgcaggcgtc gggaagacct
     5581 gggcgatgct ggcagaagcc cagcgactgc gggcgcaagg gctggatatt gtggttggcg
     5641 tggtagaaac ccatgggcgg aaagataccg ccgccatgct tgaagggctg gctgttctgc
     5701 cgcccaaacg ccaggcgtac cgtgggcggc atatcagcga gtttgatctc gatgccgccc
     5761 tcgcccgccg tccggcgctg atcttaatgg acgaactggc gcacagtaat gcgccaggtt
     5821 ctcgtcatcc taaacgctgg caggatatcg aagaactgct ggaagctggc attgatgttt
     5881 tcacaaccgt caacgttcag catctggaaa gtctgaatga tgtggtcagc ggcgtcaccg
     5941 gaattcaggt acgggaaacc gtgcccgatc cttttttcga tgccgccgac gacgtagtgc
     6001 tggtggactt gcccccggac gatctgcgcc agcggctgaa agaaggcaaa gtctatattg
     6061 ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa aggcaatctg atcgccctgc
     6121 gtgaactggc actgcgccgt actgccgatc gcgtagatga gcaaatgcgc gcctggcggg
     6181 ggcatcctgg tgaagagaaa gtgtggcaca cgcgcgacgc gatcctttta tgcatcgggc
     6241 ataacaccgg cagtgagaaa ctggtccgcg cggcggcgcg gttggcctcg cgcctgggta
     6301 gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca tcgcttaccg gaaaaaaaac
     6361 gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct gggcgcggag acggctacac
     6421 tttctgatcc agcggaagaa aaagcagtag tgcgttatgc ccgtgaacat aatctcggca
     6481 agattattct cggtcgcccg gcctcgcgcc gctggtggcg tcgggaaacg tttgctgacc
     6541 gactggcgcg catcgccccc gatctcgatc aggtgctggt cgcgcttgat gaaccacccg
     6601 cccgcacgat taacaacgcg ccggatagcc gctcttttaa agacaagtgg cgggtacaga
     6661 ttcagggatg cgtggttgcc gccgcgttat gcgccgttat cactttaatt gccatgcagt
     6721 ggctgatggc gtttgatgcc gccaacctgg tgatgctgta tctgcttggc gtggtggtgg
     6781 tggcgctatt ttatggacgc tggccttcgg tggttgctac cgtcattaat gtggtgagtt
     6841 tcgatctctt ttttatcgcc ccacgcggca cgctcgctgt ctctgatgtg caatatctgc
     6901 tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact gcgggtgtgc
     6961 gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta tatgaaatgt
     7021 cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc gaacaattta
     7081 ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac ggtaaattgc
     7141 agccgttaac acatccgcaa ggaatgaccc cgtgggacga tgccatcgca cagtggagtt
     7201 ttgataaagg cctgcctgcg ggtgcgggca ccgacacgtt acccggtgta ccgtaccaga
     7261 ttttgccgtt aaaaagcggt gagaaaacct acgggctggt ggtggtggaa ccagggaatc
     7321 tgcgtcagtt gatgatcccg gaacagcagc gcctgctgga gacgtttacg ctgttagtcg
     7381 ccaatgcgtt tgagcggctt acgctgaccg ccagtgaaga acaggcgcgg atggcaagcg
     7441 aacgggaaca gatccgcaac gctctgctgg cggcgctttc gcatgattta cgcacgccgc
     7501 tcacggtgct gttcggtcag gcagaaatct taacgctcga tctggcaagc gaaggatcgc
     7561 ctcacgcccg ccaggccagc gagattcgtc agcacgtgct gaacactacc cgactggtga
     7621 ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag aaagagtggt
     7681 taacgctgga agaagtggtc ggcagcgcgc tgcaaatgct ggaaccgggt ttatcgtcgc
     7741 ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg ccactctttg
     7801 aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg caggccgaaa
     7861 ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg gataacggcc
     7921 ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc gggaataaag
     7981 agtcggcagt accgggggta gggcttggac tggcaatttg tcgggcgata gtggatgtac
     8041 acgggggcac tattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt cgtgttacac
     8101 ttccccagca aactgcccct gaacttgaag aatttcatga ggatatgtga caaacgttct
     8161 gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg agggcgacgg
     8221 gatgcgcgtc tatgaggccg aaacgctgca acgcggcttg ctggaagcgg ctacccgtaa
     8281 gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg agtttatccg
     8341 cgacctgcgc cagtggagcg cggtgccggt gattgtgctt tccgcacgca gcgaagagag
     8401 cgacaaaatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc cgtttggcat
     8461 tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca ccgccgcgcc
     8521 cgatccgctg gtgaaatttt ccgatgttac cgtcgattta gccgcccgcg tgattcaccg
     8581 gggtgaggaa gaggtgcatc tcacgccgat tgagttccgc ctgctggcgg tgctgctgaa
     8641 taatgccggg aaagtgctca cccagcgcca gttactcaat caggtgtggg ggccaaacgc
     8701 ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa aactggaaca
     8761 ggatcccgct cgcccccgcc atttcattac tgaaaccggt attgggtatc ggtttatgcc
     8821 atgaatgttt attcaataaa tgcgcttaaa taattattaa tttattatta aacctgccaa
     8881 aaatattatt atttggcagg tttaatttct taacgcaaat ataaaacaca aaattacaac
     8941 atttaaataa caaaacagca tctaatatgg cgctattcat aattaatgat ttattatttg
     9001 gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat ggatagaata
     9061 actctacctt caactgacac agcaagaggt aaaggtaaat ggaaaataat aaccgcttaa
     9121 tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat agttttgact
     9181 ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt acaggtctcc
     9241 ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata ctgctaaccc
     9301 ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga cgagtatagc
     9361 cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg atttatttat
     9421 cagcggtgga gctttgcttt ttttccggcg tgatcgattt ctcctttgag aaattgagga
     9481 cctgctatta cctgaaataa agagatgaac aaaatgtcag aattaaaaat tgccgttagc
     9541 cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatataga cgaaagtaat
     9601 tatattgacg ttgccgccat tattttatca gtcagtgatg ttgaacgtgg aaaactcgat
     9661 gaaatagacg ctactggcta tgacattcct gtttttattg caacggaaaa tgaagaacgt
     9721 gtacccgcag aatatctgcc acgtatttct ggtgtcttcg aacattgcga atcacgtaaa
     9781 gagttttatg gacgtcagtt agaaaccgct gccagccatt atgaaacgca actgcgcccg
     9841 cccttcttcc gcgcactggt cgattatgtt aatcagggca atagtgcttt tgactgccct
     9901 ggtcatcagg ggggtgagtt tttccgtcgc catccggcag gcaatcagtt cgtggaatac
     9961 tttggcgaaa tgctcttccg ctctgacttg tgtaatgctg acgtagcaat gggcgatctg
    10021 ctgattcatg aaggtgcgcc atgtattgca caacagcatg cggctaaagt ctttaatgcc
    10081 gataaaacct atttcgtttt aaacggtact tcatcttcta ataaagtggt cttaaacgca
    10141 ttgctgacgc cgggcgatct ggtgttgttt gaccgcaata accataaatc taaccaccac
    10201 ggtgcgttgc tacaggctgg cgcgacaccg gtttatctgg aaaccgcacg taacccgtat
    10261 ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg tgagctgatt
    10321 acagaagttg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc cgttattcag
    10381 ctaggcactt acgacggtac gatttataac gcccgtcagg tggtggataa aattggtcat
    10441 ctgtgtgact acatcctctt tgactccgca tgggtcggct atgaacagtt tattccgatg
    10501 atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg tattctggtt
    10561 acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat ccataaaaaa
    10621 gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa caacgccttt
    10681 atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga tatcaacgcc
    10741 aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt aaatggcatt
    10801 aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt acctgaactg
    10861 gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga tctgcgcttc
    10921 ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga gaatcaatac
    10981 tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg taacggcgaa
    11041 tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga aaatggcgta
    11101 gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc agaagatatg
    11161 gccaagcttc agcaacttgt tgccctgctg gtacgcttcg aaaaactgct tgagtccgac
    11221 gcgccattag cagaagtgct gccttccatc tacaaacagc atgaagagcg ctacgccggt
    11281 tataccctgc gtcagttgtg tcaagaaatg catgatttgt atgcccgtca caacgtgaaa
    11341 caactgcaaa aagagatgtt ccgtaaggaa cacttcccac gcgtaagcat gaatccgcaa
    11401 gaagccaact acgcctattt acgcggtgaa gtggagctgg ttcgtctgcc ggatgcagaa
    11461 ggccgtatcg ctgccgaagg tgcgcttcct tatcctccgg gtgtgctgtg cgttgttccg
    11521 ggtgaaatct ggggtggtgc tgttctgcgt tacttcagcg ctctggaaga agggatcaac
    11581 ctgttgccag gttttgcacc ggagcttcag ggtgtctata tcgaagaaca tgatggtcgt
    11641 aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg
    11701 gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta accatactga
    11761 cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt gccgaagttg
    11821 ggacaatttc aattatctcc tggctggtga cagccgtagg ctcaatggca ctggcatggg
    11881 cattcgcaaa atgcggtatg ttcagccgta aatcaggcgg tatgggcggt tatgcggagt
    11941 atgctttcgg taaatccggt aactttatgg cgaactatac ctacggcgtc tcgctgctga
    12001 ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg ctcggcgcga
    12061 gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt gctgtggatt tgtaccgtgg
    12121 ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattaccgtg tggggggtca
    12181 ttattccggt ggtaggcctg tgcattatcg gctggttctg gtttagcccg acgctttacg
    12241 ttgattcctg gaatccgcat catgcaccgt tcttcagtgc agtaggttct tccatcgcta
    12301 tgacgctgtg ggcttttctc ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa
    12361 acccggaacg taatgtgcca atcgcggtac tcggcggtac gttaggtgcg gcggtgattt
    12421 atatcgtctc caccaacgtg attgcaggga ttgtgcctaa tatggagctg gcaaattcaa
    12481 cggcaccatt tggcctggcc ttcgcacaga tgttcacacc agaagtgggt aaagtcatta
    12541 tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc accattgccc
    12601 aggtgtttaa atcttcatct gatgaaggct acttccctaa aattttctcc cgtgtaacca
    12661 aagtggatgc accggtgcag ggaatgttga ccattgtgat tatccagagc gggttggcgc
    12721 tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt gctggttaac ctggccgtgg
    12781 tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt cagaaggtcg
    12841 ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt
    12901 atagctttta tgcgctgtac tcatccgggg aagaagccat gctgtacggt tccatcgtga
    12961 ccttccttgg ttggacactg tatggtctgg tatcaccacg ctttgaactg aaaaataaac
    13021 acggttaaaa aagcaaagta aaaaagggcg atcttgcgac cgcccttttt ttattaaatg
    13081 tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct caatctgctt
    13141 gcgatgttct tcaccgagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga
    13201 cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccaa tagaagcacc
    13261 gttgcccgga gcagcagtca ggcgcgcggt gatcgggtca cctgccaggg tgctggcgct
    13321 caccatttcc ggagacagct tagacagcgc cgctttttgt gcggaagtcg cagctgcctg
    13381 caaacggttg tagctcggcg caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg
    13441 gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga tgccgtcttt
    13501 gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc
    13561 gccaaagccg aagctgccgt cgaacagacc atcaacaaac catttgaaac ctaccggtac
    13621 ttctaccagc ttacggccca agtcattgac cacacggtcg atcatcgcag aagaaaccag
    13681 cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtagttgat
    13741 cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg
    13801 gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcagacc
    13861 cgccatcgca cactcggagg agcagtccat acggatcgca ccgtctttat cgaggtgcat
    13921 aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt tgtaatactc
    13981 gccaatgcgc ttccagtatt cgataccgga accgcccaac ggatcaacgc ccagcgtcag
    14041 gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agcccttcca cgaacggctg
    14101 caccagatcc tgctctttca catgaccgga tgccatcgct tcgtcgaggg agatacgctt
    14161 cacgcctttc aggccatcgg ccatcagtgc gttggccctg tcttccacca ctttggtgac
    14221 gttagtatca gccgggccac catttggcgg attgtacttg ataccaccat cttccggcgg
    14281 gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac
    14341 caggatggca ttggaaacgg caggcgtcgg agtgaagcca ttgttttcct gcacaatgac
    14401 atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggc
    14461 gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac gttcttcagc
    14521 aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc
    14581 acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag
    14641 tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg
    14701 gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc
    14761 cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca
    14821 tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgtg cctggcacat
    14881 gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaaa
    14941 cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat
    15001 atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt
    15061 ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg
    15121 cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg
    15181 atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct
    15241 tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa
    15301 tccagtgcag tgtcttagct gaatacaagt cgccaggagt atatgaataa atcccggcag
    15361 cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc
    15421 tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca
    15481 tgaaattgaa tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc
    15541 ttgtccatgg tctgtttggt agcctcgaca accttggcgt actggctcgc gatctggtaa
    15601 acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagagccgg
    15661 taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca ctgcagatcg
    15721 acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggca cttactgcac
    15781 tggcccccga tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc gactatcacg
    15841 tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt cagtgaatcg gacgcacaaa
    15901 ctcgccagca agcagcagca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt
    15961 ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccggta ttgtgggatc
    16021 agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcaccct gccctgttta
    16081 tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat
    16141 ttccacaggc acgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac
    16201 cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa
    16261 cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc gctgatgtat
    16321 tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag
    16381 atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga
    16441 ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc
    16501 aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg
    16561 gaagctgtcg aggcgctgaa cgagctggcg gaatcgcgca atatgagccg tagcgaactg
    16621 atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt
    16681 tccagtttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct
    16741 gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca
    16801 agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac
    16861 cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga
    16921 cattgcaaaa agcagcaaag aagatctgga agcttatgac attctgctgc tgggcatccc
    16981 aacctggtat tatggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga
    17041 gattgatttc aatggcaaac tggttgcgct gtttggttgt ggtgaccagg aagattacgc
    17101 cgaatatttc tgcgatgcat tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac
    17161 catcgttggt cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag gtctggcaga
    17221 tgacgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac tgaccgctga
    17281 acgtgtagaa aaatgggtta aacagatttc tgaagagttg catctcgacg aaattcttaa
    17341 tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca
    17401 aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc ccggtgttca
    17461 agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg
    17521 gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca
    17581 aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac
    17641 tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttccac gtttaaaaat
    17701 cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg
    17761 tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt
    17821 tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact
    17881 gacacagcaa catcaccacg atcacctgat ttgcctcgac tgcggcaagg ttatcgaatt
    17941 tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct
    18001 gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga
    18061 gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg cgggttggct
    18121 tttttataca agggaaataa gaaaagcgag agttacagct ctcacttatt tgttattact
    18181 gcgtatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat ttccggtttg
    18241 cgttgcacgc agacactgct gataatccag tacgcgtaca ctttcctggt cggcaaactg
    18301 ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt caattttttc
    18361 tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc tctttcagac gactgtcttc
    18421 ctgtggcgcg gttgattgcg cacaagctac cagccccgat gccattatgg cgatgagaat
    18481 cagttttttc atagcaaatt cctcaaacgt gcggcaggca caaaacctgc cgcgtcgggc
    18541 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
    18601 tgttaccgta accgccgccc cagcttggga tgtcagagtg gttgtcgtat tcggtgaagt
    18661 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
    18721 tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta cgcatccgga ttgctctgcc
    18781 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
    18841 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttggcgt
    18901 tgaagtcgga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
    18961 gagtcatacg ttgcaggaag tatccctgct gaccgtcagc cttaacccag gtgccttcga
    19021 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaggcgg
    19081 tgccgtcata aaggtcgttg acgctgcgat cgtcaacttt atcgcgcgta ccatagaact
    19141 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
    19201 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
    19261 agtcgtattt cgccccaagg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
    19321 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
    19381 aactcaacgc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
    19441 tacctggcat aaagctccag tgcggcgcta acagcgtttg accagttggc tgaatgtaac
    19501 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
    19561 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
    19621 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
    19681 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    19741 agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    19801 gctgccagta atatataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    19861 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcgatagcc agcgccagcg
    19921 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    19981 accaaaggtg aacagcgaaa aaataaaaat taaaaaagta gcgttgagaa aagtctcgct
    20041 gactacagga acagactatt tttcgcgacg cgaattatca atctctttct gtaaccagaa
    20101 tgtaagaaca tgagcaaccg cacatatttt gatgattttt gttacaaggc ttaagtttgc
    20161 ggttgaaagg cttgcagaag aataaaagcg tgaatttcca gcaagatgaa agcggttaca
    20221 ttttatttaa ttatggtttg tgattttaaa tttattttat ggataaattg cgaagcggaa
    20281 ttaattcgcg aagaaaagca gaaaaaacgc cgcagagtgc ggcgtttggg attgtttgct
    20341 atttcttgcc ggatgcgacg taaatgcctc acccggcctg gtagaactta aaattactcg
    20401 cctactttcg cccaggtatc acgcagccca acggtgcggt taaataccgg tttttccgct
    20461 gtggaatggc ggctgtcgag gcagaagtaa ccttcacgct caaactggaa tgctttaccc
    20521 gcaaccgcat ctttcagcga cggttcagca aagccctgtt tgatcaccag cgattccggg
    20581 ttaatcaccg acaggaaatc atccgcagca ccagggttcg gcacgctgaa cagacggtcg
    20641 tacagacgga tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat aacacctttg
    20701 actttacgac catctgccgg atctttgctt aaggtgtcgg cgtcataagt acagaagatg
    20761 gtggtgatat tgccttcggc atctttctcc acgcgttcag ccttaatgac gtaagcatta
    20821 cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg
    20881 aaatcggcgc gatcgatcca aatctcaccg ctaaacggca cctgacgact ccccatttcc
    20941 ggtttgttcg gatggttcgg catggtgacc atttcgcctt cgccctgata gttttcgata
    21001 accagtttca ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt gagatcttca
    21061 cggatgcagg attccagcga tgccatctca atggtgttgt cctgcttggt cacgccgatg
    21121 cgtttgcaga actcacgaat agaagcagca gtgtaaccac gacgacgcag accggaaatg
    21181 gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggtcaccag caggttcagc
    21241 ttacgcttgg acatcacagt gtattccaga ttcaggcgcg agaattcgta ctggcgcggg
    21301 tgaacaggaa tggtgatgtt gtccagcacc cagtcgtaca ggcgacggtt gtcctggaac
    21361 tcaagcgtac acagagagtg cgtaatacct tccagggcat cgctgatgca gtgggtgaag
    21421 tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc aaatttaata
    21481 cggtacagca ccggatcgcg catcacgata aacggtgaag ccatgtcgat tttcgcacgc
    21541 aggcaggctt taccttcttc aaaaccaccg gcacgcattt tttcgaacag cgccaggttc
    21601 tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gttgcgtcag ggtgccgcgg
    21661 tattcgcgga tctgttccgg cgtcagttca tcaacgtacg ccaggccttt attgatcagt
    21721 tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac gttaccagac
    21781 cagtgaaaac ctaaccactc tacgtcgttt ttgatcgact caacatactc gatatcttct
    21841 tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc
    21901 ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt cggctccggc
    21961 gggaaacggg tgtgtactgt ggtgtgctta ccactggcca gatcttcatc gatgatctga
    22021 cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa
    22081 caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt agttttttaa
    22141 aattgataat ctaacaagga ttatggggat gattgatgaa aaaaaaagcg gcagagaaga
    22201 tctccaccgc ttgtcattgt tggatgcgac gctcaagcgt cgcatcaggc ataaagcaga
    22261 ttactttttg atttcataca gcggtgtttg acccgccaca acatggccct gagctttaat
    22321 gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca tcgagcgggc
    22381 gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc ttacctgcgc
    22441 accctcttcc accagacgtt taaagccttt accttccagc gctacggtgt cgatacccat
    22501 atggacgacg atctccgcgc ctttttcggt ttccaggcag aacgcgtggt tggtgttgaa
    22561 gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg gtttcaccgc
    22621 cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac
    22681 gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg gtacagcctg
    22741 cggttttgct acaggcgcgg cagttgccgg
//
LOCUS       DAFATA010000072        22734 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFATA010000072 DAFATA010000000
VERSION     DAFATA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.073"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..785)
                     /gene="fadI"
                     /locus_tag="LGJ20_003248"
     CDS             complement(<1..785)
                     /gene="fadI"
                     /locus_tag="LGJ20_003248"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="FadI; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadJ; similar to
                     FadA2B2 complex; functions in beta-oxidation of fatty
                     acids during anaerobic growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBK2387271.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQGTAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDR"
     gene            complement(965..1249)
                     /locus_tag="LGJ20_003249"
     CDS             complement(965..1249)
                     /locus_tag="LGJ20_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBK2387272.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            1621..2961
                     /gene="fadL"
                     /locus_tag="LGJ20_003250"
     CDS             1621..2961
                     /gene="fadL"
                     /locus_tag="LGJ20_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBK2387273.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            3327..4385
                     /locus_tag="LGJ20_003251"
     CDS             3327..4385
                     /locus_tag="LGJ20_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387274.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKETISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAK
                     YQNEINQLSAPGVNFDDI"
     gene            complement(4567..5322)
                     /gene="mlaA"
                     /locus_tag="LGJ20_003252"
     CDS             complement(4567..5322)
                     /gene="mlaA"
                     /locus_tag="LGJ20_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBK2387275.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            5616..6548
                     /locus_tag="LGJ20_003253"
     CDS             5616..6548
                     /locus_tag="LGJ20_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK2387276.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            6624..6698
                     /locus_tag="LGJ20_003254"
     tRNA            6624..6698
                     /locus_tag="LGJ20_003254"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6657..6659,aa:Arg,seq:cct)
     gene            complement(7140..8387)
                     /locus_tag="LGJ20_003255"
     CDS             complement(7140..8387)
                     /locus_tag="LGJ20_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HBK2387277.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8459..9373)
                     /locus_tag="LGJ20_003256"
     CDS             complement(8459..9373)
                     /locus_tag="LGJ20_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBK2387278.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9589..11022
                     /gene="cscA"
                     /locus_tag="LGJ20_003257"
     CDS             9589..11022
                     /gene="cscA"
                     /locus_tag="LGJ20_003257"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK2387279.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(11030..12088)
                     /locus_tag="LGJ20_003258"
     CDS             complement(11030..12088)
                     /locus_tag="LGJ20_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK2387280.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            12256..12474
                     /locus_tag="LGJ20_003259"
                     /pseudo
     CDS             12256..12474
                     /locus_tag="LGJ20_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12492..13820
                     /gene="dsdA"
                     /locus_tag="LGJ20_003260"
     CDS             12492..13820
                     /gene="dsdA"
                     /locus_tag="LGJ20_003260"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBK2387281.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13928..15466)
                     /gene="emrY"
                     /locus_tag="LGJ20_003261"
     CDS             complement(13928..15466)
                     /gene="emrY"
                     /locus_tag="LGJ20_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBK2387282.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(15466..16629)
                     /gene="emrK"
                     /locus_tag="LGJ20_003262"
     CDS             complement(15466..16629)
                     /gene="emrK"
                     /locus_tag="LGJ20_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBK2387283.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQF"
     gene            17045..17659
                     /gene="evgA"
                     /locus_tag="LGJ20_003263"
     CDS             17045..17659
                     /gene="evgA"
                     /locus_tag="LGJ20_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBK2387284.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            17664..21257
                     /gene="evgS"
                     /locus_tag="LGJ20_003264"
     CDS             17664..21257
                     /gene="evgS"
                     /locus_tag="LGJ20_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBK2387285.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATIEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(21313..22458)
                     /gene="yfdE"
                     /locus_tag="LGJ20_003265"
     CDS             complement(21313..22458)
                     /gene="yfdE"
                     /locus_tag="LGJ20_003265"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBK2387286.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(22532..>22734)
                     /locus_tag="LGJ20_003266"
     CDS             complement(22532..>22734)
                     /locus_tag="LGJ20_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK2387287.1"
                     /translation="GMACHLNSEHLQMMVLAGALPPAFSGIIIASRFNVYTRTGTASL
                     AVSVLGFVVTAPLWIYVSRLVS"
BASE COUNT         6570 a         5304 c         4989 g         5871 t
ORIGIN      
        1 cgatcaaacg ccggacgcag ctttgcgtaa tcggcaagcg aggaattacc gcgaatattg
       61 ttgtcttcga caagcggttg tttataagga gggataaagg cagtcatcac ctcttctttg
      121 agttttccgt ctgaccatgc ctgagcggca cgctgatgcg aacggtgcgc taatgcatct
      181 tgctgttctc gggtgatgcc gtaggttttc gccatttgtt cggcggtgtc gcccatccgc
      241 aagccagtag aatattctgc taccgcaggc ggtacgggca ttaagtcgcg caaacgcagg
      301 cgagagaaga gtttcaggcg ctggctcatg gttcgagctt tgttgacatc aaccagcacg
      361 cgcgccagtt ttttactgac gccaattggc aatacggaag aggaatctgc cccaccggca
      421 atccccgctc ggatagttcc cgccatcagg ctttctgcga cgtttgcaac cgcctggaaa
      481 ctggtagcgc aagcgcggct gacgctgtaa gcatcggtat gcacattcat tccggtaccg
      541 agaacaattt cacgcgcaat gttgggggct tcaggcattt gtacgacctg accaaagacc
      601 agctgttcaa tcacttcagc ggggatctcg ctgcgtgcca gcagttcgcc taccaccatc
      661 ttccctaaat caaccgcggg aatgccatga aaagccgtcc cctggcgggc aaaaggcgta
      721 cgtaaaccgc taacaatggc gatacgatcg ccctggcggg taaccagcgg taaaacctga
      781 cccataacac tcccctgtaa aaaaataaat aaagtggtct gacctgatca tagtcttaac
      841 cattttttta catttagcca agcggagaaa aaagaaagtg ggagctatga cacagtgaag
      901 acaggagaaa gaaaagaaaa cgcccctgcc agcaactgac aggggcgaac gtacccgaga
      961 ggaattaacg cagacccagc tggaagatca gcatttcagc ttcgcaggca aaagtgaaat
     1021 cgatatccag gcgcacaccg tcagactctt cagtgaaagt cggggagatt ttgcacggtt
     1081 cggattccac gctacgggct ttttcagtca gcgccgccag cgtttgttct gcttctgcgc
     1141 ggtttgcaaa cacgcggctg taagacgcgg tgcagtcgga gttgtccata atggtgccaa
     1201 catccataca gcagcaaacc ggggtttcat cagcactaca tttactcatc gttgatttcc
     1261 tctgtatgtg cacccaaggt gccagataaa cgttgtggat attttacgct tccggaaagt
     1321 gctgctccag ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg
     1381 atctaaaaca aaattcaccc gaatccgcga gtgcgcaacc tccaaatttt gccagctgga
     1441 tcgcgtttct tagatcatat ttgaaaaaag atggaaacat acttgcaaca ttccagctgg
     1501 tccgacctat actctcgcca ctggtctgat ttctaagatg tacctcagac cctacacttc
     1561 gcgctcctgt tacagcacgt aacatagttt gtataaaaat aaatcattga ggttatggtc
     1621 atgagccaga aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc acttatctcc
     1681 acccaggcct ggtcggcagg ctttcagtta aacgaatttt cttcctctgg cctgggccgg
     1741 gcttattcag gggaaggcgc aattgccgat gatgcaggta acgttagccg taaccccgca
     1801 ttgattacca tgtttgaccg cccgacattt tctgcgggtg cggtttatat tgacccggat
     1861 gtgaatatca gcggaacgtc tccgtctggt cgtagcctga aagccgataa catcgcgcct
     1921 acggcatggg ttccgaacat gcactttgtt gcaccgatta acgaccaatt tggttggggc
     1981 gcttctatta cctctaacta tggtctggca acagagttta atgatactta tgcaggcggc
     2041 tctgtcgggg gtacaactga ccttgaaacc atgaacctga acttaagcgg tgcgtatcgc
     2101 ttaaataatg catggagctt tggtcttggt ttcaacgccg tctacgctcg cgcgaaaatt
     2161 gaacgtttcg caggcgatct ggggcagttg gttgctggtc agattatgca atctcctgcc
     2221 gggcaaactc ctcaagggca agcattggca gctaccgcca acggtatcga cagtaatacc
     2281 aaaatcgctc atctgaacgg taaccagtgg ggctttggct ggaacgccgg aatcctgtat
     2341 gaactggata aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa aattgacttc
     2401 aaaggtaact acagcagcga tcttaatcgt gcgtttaata actacggttt gccaattcct
     2461 accgcgacag gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc tgaaatgtgg
     2521 gaagtgtcag gttataaccg tgttgatcca cagtgggcga ttcactatag cctggcttac
     2581 accagctgga gtcagttcca gcagctgaaa gcgacctcaa ccagtggcga cacgctgttc
     2641 cagaaacatg aaggctttaa agatgcttac cgcatcgcgt tgggtaccac ttattactac
     2701 gatgataact ggaccttccg taccggtatc gcctttgatg acagcccagt tccggctcag
     2761 aatcgttcta tctccattcc ggaccaggac cgtttctggc tgagtgcagg tacgacttac
     2821 gcatttaata aagatgcttc agtcgacgtt ggtgtttctt atatgcacgg tcagagcgtg
     2881 aaaattaacg aaggcccata ccagttcgag tctgaaggta aagcctggct gttcggtact
     2941 aactttaact acgcgttctg ataacacgtt cgcctggata aagtcacctg catagcaggt
     3001 gactttaact cccccacttc accgaaagta gtgtcctcat tgcttacctc ctgagttttg
     3061 caaacagcct gatggcagct tgccctttca aatcaataag cggtatccac agacaaatct
     3121 gaagggaaag gcatattttc aggcgttctg ctcgtccttc tcaaagagtt tacttttctg
     3181 aatttccagg atactcaccc cccctggcta ttgtgcgctc atacactcaa attaaagata
     3241 ggttctaaat aaatgagcgt tttttgatag tctatttcat taggtaatat atatttgtaa
     3301 caaatcaatc aaaatggaat aaaatcatgc taccatctat ttcaatcaac aataccagcg
     3361 caacttaccc agaatccatt aatgaaaata acaatgatga agttaatgga ttagtacaag
     3421 agctcaaaaa cctttttaat ggtaaggaag gaataagcac ctgtattaaa catctacttg
     3481 agcttataaa aaacgccata cgagtaaacg acgatcctta tagatttaat attaataatt
     3541 cctcagttac ttatattgat attggctcca atgatacaga ccatattact attggtatcg
     3601 acaaccaaga accaatagaa ttacctgcga actataaaga caaagaactc gtccgtacta
     3661 tcattaatga caacatagtt gagaagactc atgatatcaa taacaaggaa atgatcttca
     3721 gcgcattaaa agaaatatat gatggagatc ctggttttat cttcgataag atatcacaca
     3781 aactcagaca tacggtaacg gaatttgatg agagcgggaa tagcgaacca acggacttat
     3841 ttacctggta cggtaaagat aaaaaaggcg actctctcgc tattgtaatt aaaaataaaa
     3901 acggaaatga ttacttatct ctcggttact acgatcagga cgactaccac attcaaagag
     3961 gaattcgtat taatggtgat agtctcaccc aatattgtag tgaaaaagcc aggaatgctt
     4021 cagcgtggtt tgaaagcagt aaagctatca tggcagaatc atttgcaaca ggttccgatc
     4081 atcaggttgt aaacgagctc aacggggaaa gactgagaga acctaacgaa gtttttaaac
     4141 gtttaggtcg agcaataaga tataattttc aagtggacga tgcaaaattt agacgcgaca
     4201 atgtaaaaga aacaatttct aatttattcg ctaacaaagt tgacgttgac catcctgaaa
     4261 ataaatacaa agactttaag gatctcgaag ataacgttga aaaaagactt caaaatcgcc
     4321 aggcgaaata tcaaaatgaa attaatcaac tatctgcgcc aggtgttaat tttgatgata
     4381 tttaaaatta tttttccagt tttttgattt ttcaacgcct gaatatcgga aatagtcagt
     4441 gacgaacatc agtattttta catcattacg tccgggtcat tgtcggttta tctcctttta
     4501 cttgtggtaa ttgagggata acaacaaaaa aggtgagtat tgcgactcac cttttttatt
     4561 tgtttcttat tcagaatcaa tatcttttaa atcatcctga atcgcttgtg cgttcggatt
     4621 ttcctgcggt ttgagttcgc cgccattagc gatgaaatca tgacgctgga agtacgcttc
     4681 gcgcaccata atataaggat cggacgactg acgcagcaga ccatcggaat ccagcagctg
     4741 agcgcgggtt tcgatccctt caagcgtcca tttacccaca gacatcggcc aggtcagcca
     4801 ggaaagaacc gggtaaagac catccgccat atcaccaccg tcatcacgca gcgtgaagct
     4861 accgtagaac ggtaactgaa cgtaaggccc ataacccacg ccataatgac caagcgtact
     4921 accgaagcgg tgaggttcag tccgttgcag tttcgggttc gccatccctg caacatcaat
     4981 aaagccgccc atccccaaaa tggtgttcag gaaaaagcgg gtaaagtgga ccatcccctg
     5041 ataagggtcg ccctgcaaga agtagttaac catcaccgca ggttcttcaa ggttgccagt
     5101 aaagttgctc aaaccattac gcgccggttg cggaacataa tcacgccagg cgacagcgac
     5161 cggtcgaaca atatacgggt ctaatacgtt gaagttgaag ttgtacatgg tgcggttgaa
     5221 cccttctaac gggtcagaac gcccttgctg atctgtaccg gaactcgcac accccaccag
     5281 aagcgtagtt cccagagcaa gcgccgacag gcgaagcttc ataaatgtct ccctgttttt
     5341 ttatggctta tgcagtttgc catccatgac ggaacgatac cgtatccgcc tgtttaggtg
     5401 tgggcgattg taacagcacg tcaactgatg tccagacgcc ctgatttgct gatttgatca
     5461 tagcctggta atcgccgccc tgtaggctac ttgattctat agaaacagaa aaaggcaaac
     5521 gcagcctttt ctacattttc agagtaactc ccgccagttg cgagcaaaaa agccgctacg
     5581 ctttagctat acgtgctaat tcaagagaag agaccatgga caacgacaaa attgatcaac
     5641 acagcgacga aattgaagtt gagagcgaag aaaaagagcg cggcaaaaaa atagaaatag
     5701 atgaagatcg actcccctcc cgggcgatgg caattcatga gcatatccgc caggatggtg
     5761 aaaaagagct ggaacgcgac gcaatggcgc tactgtggtc agccattgcg gcgggtctgt
     5821 cgatgggcgc ttcgctactg gcaaaaggga tatttcatgt cgaactggaa ggagtgccag
     5881 gcagcttctt actggagaat ctcggttata cctttggttt tattatcgtc attatggccc
     5941 gccagcaatt atttaccgaa aacaccgtga ctgcggtact acccgtcatg caaaaaccga
     6001 caatgagcaa cgtcggctta cttatgcgat tatggggcgt cgtgctgctg ggtaatattc
     6061 tcgggacagg tattgctgca tgggcatttg aatatatgcc tatcttcaat gaagaaactc
     6121 gcgatgcatt tgtcaaaatc ggcatggatg tgatgaagaa cacccccagc gagatgtttg
     6181 ccaacgcgat catttccggc tggctgatcg ccactatggt ttggatgttt cctgctgcgg
     6241 gtgcggcaaa gattgtggtg attatattga tgacctggct tattgcactg ggtgacacca
     6301 cccacatcgt tgtcggttct gttgaaatcc tctatctggt gtttaacggc acgctgcact
     6361 ggagcgattt catctggccc ttcgcactac ctactttagc ggggaacatc tgcggcggca
     6421 cctttatctt cgcgttaatg agtcatgcac agattcgtaa cgacatgagc aacaagcgta
     6481 aagcagaagc acgccaaaaa gcagaacgtg cggaaaacat taagaaaaat gataaaaacc
     6541 cagcataaat ggcgagggtt taagcaatcg agcggcagcg tacttacccc gcagtccatt
     6601 agcgggtata ctcatgccgc attgtcctct tagttaaatg gatataacga gcccctccta
     6661 agggctaatt gcaggttcga ttcctgcagg ggacaccaga tacccttcaa acgaaatcta
     6721 ccttcacccc gtaaaagatg ggtttggcag cacacttgcc ttatatctac tcatttttac
     6781 tgcaacaggt tgaaatctca gcactgtcag aaagcgctga tgactaaaca gccctgggcc
     6841 gggcgatgta accatcacac agaatcctga tagcgaaata tggcgtgact cgatacttca
     6901 ctccgcaatg cattccttga tgaattcgca ggaccgtgat acacgggaca ggtcactgaa
     6961 tgacgacaat gtcctggaaa tcagcgaacc gcgcatctga agtacatttg agcgactgta
     7021 ccagaacatg aatgaggcgt ttggattagg cgattattag cagggctaag cattttacta
     7081 ttattatttt ccggttgagg gatatagagc tatcgacaac aaccggaaaa agtttacgtc
     7141 tatattgctg aaggtacagg cgtttccata actatttgct cgcgtttttt actcaagaag
     7201 aaaatgccaa atagcaacat caggcagaca atacccgaaa ttgcgaagaa aactgtctgg
     7261 tagcctgcgt ggtcaaagag tatcccagtc ggcgttgaaa gcagcacaat cccaagcgaa
     7321 ctggcaattt gaaaaccaat cagaaagatc gtcgacgaca ggcgcttatc aaagtttgcc
     7381 acgctgtatt tgaagacgga tatgacacaa agtggaacct caatggcatg taacaacttc
     7441 actaatgaaa taatccaggg gttaacgaac agcgcgcagg aaaggatacg caacgccata
     7501 atcacaactc cgataagtaa tgcatttttt ggccctaccc gattcacaaa gaaaggaata
     7561 atcgccatgc acagcgcttc gagtaccacc tggaatgagt tgagataacc atacaggcgc
     7621 gttcctacat cgtgtgattc gaataaacct gcataaaaga caggaaaaag ttgttgatca
     7681 aaaatgttat agaaagacca cgtccccaca ataaatatga cgaaaaccca gaagtttcga
     7741 tccttgaaaa ctgcgataaa atcctctttt tttacccctc ccgcatctgc cgctacgcac
     7801 tggtgatcct tatctttaaa acgcatgttg atcatcataa atacagcgcc aaatagcgag
     7861 accaaccaga agttgatatg gggactgata ctaaaaaata tgccggcaaa gaacgcgcca
     7921 atagcatagc caaaagatcc ccaggcgcgc gctgttccat attcgaaatg aaaatttcgc
     7981 gccatttttt cggtgaagct atcaagcaaa ccgcatcccg ccagataccc caagccaaaa
     8041 aatagcgccc ccagaattag acctacagaa aaattgcttt gcagtaacgg ttcataaacg
     8101 taaatcataa acggtccggt caagaccagg atgaaactca tacaccagat gagcggtttc
     8161 ttcagaccga gtttatcctg aacgatgccg tagaacatca taaatagaat gctggtaaac
     8221 tggttgaccg aataaagtgt acctaattcc gtccctgtca accctagatg tcctttcagc
     8281 caaatagcgt ataacgacca ccacagcgac caggaaataa aaaagagaaa tgagtaactg
     8341 gatgcaaaac gatagtacgc atttctgaat ggaatattca gtgccataat tacctgcctg
     8401 tcgttaaaaa attcacgtcc tatttagaga taagagcgac ttcgccgttt acttctcact
     8461 attccagttc ttgtcgacat ggcagcgctg tcattgcccc tttcgccgtt actgcaagcg
     8521 ctccgcaacg ttgagcgaga tcgataattc gtcgcatttc tctctcatct gtagataatc
     8581 ccgtagagga cagacctgtg agtaacccgg caacgaacgc atctcccgcc cccgtgctat
     8641 cgacacaatt cacagacatt ccagcaaaat ggtgaacttg tcctcgataa cagaccacca
     8701 caccttctgc acctttagtc accaacagca tggcgatctc atactctttt gccagggcgc
     8761 atatatcccg atcgttctgt gtttttccac tgataagtcg ccattcttct tccgagagct
     8821 tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa gcggagcaaa tgctcgtctt
     8881 gccatagatc ttcacgaata ttaggatcga agctgacaaa acctccggca tgccggatcg
     8941 ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc agacaacgca attgaacaga
     9001 gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt cgtctctaaa aaaagatcgg
     9061 cactggggcg gaccataaac gtaaatgaac gttccccttg atcgttcaga tcgacaagca
     9121 ccgtggatgt ccggtgccat tcatcttgct tcagatacgt gatatcgact ccctcagtta
     9181 gcagcgttct ttgcattaac gcaccaaaag gatcatcccc cacccgacct ataaacccac
     9241 ttgttccgcc taatctggcg attcccaccg caacgttagc tggcgcgccg ccaggacaag
     9301 gcagtaggcg cccgtctgat tctggcaaga gatctacgac cgcatcccct aaaacccata
     9361 ctttggctga catttttttc ccttaaattc atctgagtta cgcatagtga taaacctctt
     9421 tttcgcaaaa tcgtcatgga tttactaaaa catgcatatt cgatcacaaa acgtcatagt
     9481 taacgttaac atttgtgata ttcatcgcat ttatgaaagt aagggacttt atttttataa
     9541 aagttaacgt taacaattca ccaaaattac ttaaccagga tgattaaaat gacgcaatct
     9601 cgattgcatg cggcgcaaaa cgccctagca aaacttcatg agcaccgggg taacactttc
     9661 tatccccatt ttcacctcgc gcctcctgcc gggtggatga acgatccaaa cggcctgatc
     9721 tggtttaacg atcgttatca cgcgttttat caacatcatc cgatgagcga acactggggg
     9781 ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca tgagcctatt
     9841 gcgttagcgc caggagacga taatgacaaa gacgggtgtt tttcaggtag tgctgtcgat
     9901 gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg tgcaggtaat
     9961 gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat tcatttcgag
    10021 aaacagggtg tgatcctcac tccaccagaa ggaatcatgc acttccgcga tcctaaagtg
    10081 tggcgtgaag ccgacacatg gtggatggta gtcggggcga aagatccagg caacacgggg
    10141 cagatcctgc tttatcgcgg cagttcgttg cgtgaatgga ccttcgatcg cgtactggcc
    10201 cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag ccttggcgat
    10261 cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag ttaccgaaat
    10321 cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt tgcacaatcc
    10381 gggcatttta ctgaacttga taacgggcat gacttttatg caccacaaag ctttttagcg
    10441 aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc aatgccctca
    10501 aaacgtgaag gatgggcagg ctgcatgacg ctggcgcgcg agctatcaga gagcaatggc
    10561 aaacttctac aacgcccggt acacgaagct gagtcgttac gccagcagca tcaatctgtc
    10621 tctccccgca caatcagcaa taaatatgtt ttgcaggaaa acgcgcaagc agttgagatt
    10681 cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca gctcggcact
    10741 ggaatgcggc tgtatattga taaccaatct gagcgacttg ttttgtggcg gtattaccca
    10801 cacgagaatt tagacggcta ccgtagtatt cccctcccgc agcgtgacac gctcgcccta
    10861 aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga agcggtgatg
    10921 agtagtcgaa tctatccgca gccagaagaa cgggaactgt cgctttatgc ctcccacgga
    10981 gtggctgtgc tgcaacatgg agcactctgg ctactgggtt aacataatat caggtggaac
    11041 aacggatcaa cagcgggcaa gggatccgcg tcactcttcc cccttcacga ccttcaataa
    11101 tatgcaatgc agcttcccgc ccgataatgt catgtggaag ctgaattgtg gtcagcggcg
    11161 gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc cattacggcg acatcctgcg
    11221 ggattcgtac ccccttcgcc agaagaacct gataagccac aaaggctgcg cgatcgttac
    11281 cacatatcag aacatcaaaa tctggtttgc ccgatttgaa gtggtcattg agtaaacttg
    11341 cgagatcggt gtagtgatca tcacctgttg ccatgtgaaa ttgtttcacc tcagccagat
    11401 ctcgtccagc atcacgccag gcctgctcaa atccctgccg acgataccct gttgccaacg
    11461 cactttccgg tagccagaag cataacggtt gacgatagcc cgccgcgagc aaatgctgtg
    11521 ttgattcata ttgtgcagtg taatcatcag ggatataact gggtaacgct gggtcatccg
    11581 ccacacagtt cgccaataca atattttcac catacagaga ctcaggcagc gtgatatgtc
    11641 gcagccccat tgtagtatag ataatgccat ccggacggtg ggcaagcagc tgacgtgccg
    11701 cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa actattccag ccgaactcgc
    11761 tggcggtttg ctcaatggca agcagaatat caacagagaa aggagtggta gccgtgtcct
    11821 gcgccagcac ggcgagagtc gacggcttac gtccttgagc gcgcatctta cgggcggaaa
    11881 gatcaggaac ataattcagg gtctggattg cctgcaatac gcggtcacgc gttgcaggac
    11941 gcacagattc tgcattatgc atcacccggg agactgtcat catcgacact cccgccaggc
    12001 gtgcgacatc ctttaatgaa gccataccca agccgtttgc cgtaaaacgg gcactgtagc
    12061 agaaacagac gtcactggcg agatccaacg ccctatcacc tgacacagca atacaataaa
    12121 aaataacaat aattcccgga caattgtccc caattccgcc tctgttctcg ccaacgagta
    12181 accatatagg caagtaagta aaacatgcca actggcatcg ccaacctgga gaagaaaata
    12241 taaatatgga tcattgtgct gccgctgtat cccgacatca gcccggaaat tattgcgatt
    12301 gctatcggtt caggtgcaat tggctgcacg atcgttacgg actcgctttt ctggctagtg
    12361 aagcaatatt gcggcgctac gctcaatgaa acatttaaat actatacgac agcgacattt
    12421 atcgcttcag tcgtcgctct ggcgggcaca ttcctgctgt catttatcat ctaagcgcaa
    12481 agagacgtac tatggaaaac gctaaaatga actcgctcat cgcccagtat ccgttggtaa
    12541 aggatctggt tgctcttaaa gaaaccacct ggtttaatcc tggcacgacc tcattggctg
    12601 aaggtttacc ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat
    12661 cccgttttgc accctatctg gcaaaagcat ttcctgaaac tgctgccact ggggggatta
    12721 ttgaatcaga actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc
    12781 aaccgatcag cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccggctcca
    12841 taaaagcacg cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag
    12901 cggggttgct gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt
    12961 tctttagcca atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca
    13021 ttatgagcgc ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat
    13081 ggaaaaaagc gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg
    13141 gtgttgccgt cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg
    13201 atgacgaaaa ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag
    13261 cgcaatttgc ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc
    13321 cgtgtggtgt tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg
    13381 atcatgttca ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc
    13441 atacaggatt acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag
    13501 cggatggcct tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gagcgtctgc
    13561 tggatggctt ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc
    13621 aggaagaagg tattcgtctt gaaccttcag cactggcggg tatggccgga cctcagcgcg
    13681 tgtgtgcatc agtaagttac caacagatgc acggtttcag cgcagaacaa ttgcgtaatg
    13741 ccactcatct ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat
    13801 atctggcaaa aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga
    13861 gcgatgctgc cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct
    13921 tttatcatca cccaatgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg
    13981 taagtaagat aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg
    14041 actgctgggt tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt
    14101 tatccataat ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca
    14161 actgactatg gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa
    14221 ctgatcctga caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat
    14281 ctggcaagcc tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc
    14341 cctggaaaaa ctgtggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag
    14401 aacgccagta atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt
    14461 ctattttgtt gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg
    14521 gcgcataagc aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg
    14581 gcataaggac gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa
    14641 tggtgaagtt acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact
    14701 cccaaatgac taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac
    14761 tcgagttgaa ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac
    14821 cgagcactaa cagggtcagt cctggtagat tcattttgac cggtgaagtt tcagtttctc
    14881 ttcctttaag taaggttaag cataatgtca ggacgataat ccccataggg acattgatta
    14941 aaaatatcca accccagcta aagttatcgc aaatataacc gcccaatatc ggcccacata
    15001 tcggagcgat aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggtg
    15061 gataattcct taataataaa ctctgtgaca gtggaattaa cggccccgcc attaatccct
    15121 gaacgactct aaaaaatatc agcacatcaa gattggtcga taaactacac attaaggaag
    15181 acagcgaaaa aaaagtgact gaaagtaaaa ataaccttaa ttcgcctatt ctttgtgcca
    15241 gcctgccggt aacagggatc gcaatggcat ttgctacgcc aaacgaggtg ataacccagg
    15301 tgccttcgtc tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa
    15361 tagtggaatc caacatttgc ataaacgtcg ctaatgacaa tgcaatagta acgcaccata
    15421 acgtcccacc ggttaatggt gccggagtcg atttagtgat tgccattaaa attgtccatt
    15481 atgcgaaata atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt
    15541 actggtataa gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc
    15601 ttcgttcttc gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat
    15661 gagttctttt ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt
    15721 ccctgtcgca ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc
    15781 tgtcacccga ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga
    15841 ttgaccaatc cgtacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg
    15901 acgtgccggt acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac
    15961 acttcgctgg gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa
    16021 ccaggcttct ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt
    16081 gttcattact aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt
    16141 gctacttatt aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt
    16201 cgctaacggc actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga
    16261 agcgacttcg gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat
    16321 atttgccaga ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga
    16381 aactaaaatg tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact
    16441 acctgagact tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact
    16501 aatcatgtct tctaattcca ttgaccaata ggcataggca cctgaaaacg caataaataa
    16561 aactaccgcc aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat
    16621 ctgttccact attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc
    16681 attcattctt catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg
    16741 cctaatttaa atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac
    16801 aattcttacg cctgtaggat tagtgagaag acttatagtg ctaacttgaa actataaatc
    16861 atcggtataa tccctgattt tattgttgac atttcattta tgccgactat ttatatggta
    16921 tacttgtcga attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga
    16981 tgacgcctta tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata
    17041 atctatgaac gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt
    17101 attgatcaaa aacgatattg aaatcttagc ggagttgact gaagggggaa gcgccgttca
    17161 gcgggtggaa acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa
    17221 cggtatccag gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt
    17281 ctccgctaaa aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt
    17341 cgtgagtaaa aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg
    17401 ctactgctat ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca
    17461 aaaactcgac tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa
    17521 ggataataat gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa
    17581 aagtcgcctg atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca
    17641 acgtaacaaa atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg
    17701 gtctttggtc gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta
    17761 gtaacaaccg tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag
    17821 cgagcaaaaa aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata
    17881 ccgattcgca gcaacaggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag
    17941 cgttaaatat caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc
    18001 ttgcagaagg tgaagtcgat atagtgttat cacatttagt tacttcgccg cctcttaata
    18061 gtgacattgc tgcaaccaaa ccattgataa ttacctttcc ggcgctggta accacccttc
    18121 acgactcaat gcgaccgctt acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt
    18181 accccccaga cgaggtaatt catcaatcgt ttccaaaagc aacaattatc tcttttacaa
    18241 atttatatca ggcattagca tccgtatcag ccggacagaa tgattacttt attggtagta
    18301 acatcattac cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat
    18361 attataactc gccgcgtcaa tataattttt tcttgaccag aaaagaatcc gtcattctta
    18421 atgaagtact caatagattt gttgatgctt taacaaatga agttcgctat gaagtttcac
    18481 aaaattggct tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac
    18541 atgaaaaaca gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc
    18601 ctccctattc tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta
    18661 atattattac cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc
    18721 atgccggaac acagcttaac cccggtggat gggatataat acctggcgct atttatagtg
    18781 aagatcgaga aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg
    18841 tcatgcaaaa agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc
    18901 catattatta tgagctgcat tcgcaattaa aagagatgta tccggaggtt gaatggatac
    18961 aggtcgataa tgccagcgct gcatttcaca aggttaagga aggtgaactt gatgctctgg
    19021 tcgcgacaca gttaaattcg cgttacatga tcgatcatta ctatcctaat gaactttatc
    19081 attttcttat tcctggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac
    19141 cggaacttaa ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc
    19201 gcctgacgga aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctatata
    19261 gcgagcaatt ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat
    19321 ggggattcta cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa
    19381 accaaatatc attccgaaaa gcactctcgg attccttacc gaatccaact tatgttgtaa
    19441 actggcaagg taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt
    19501 actacaaaaa tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt
    19561 ctaatgcgca tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat
    19621 ttgaaattga taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc
    19681 ttcctgcaag tgacaatgca gtatatattt gtggttggca agatattact gaaacgcgtg
    19741 atctaattaa tgcactcgag gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa
    19801 gtcagtttct ggcaacgatg agtcacgaaa taagaacacc aataagctct attatgggct
    19861 tcctggaact tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac
    19921 ttgcctacgc caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca
    19981 aaattgaatc gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc
    20041 agaacacttg tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca
    20101 gcagtacatt tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct
    20161 tatcaaattt actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga
    20221 cctccctggt tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg
    20281 gaagtggatt atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg
    20341 caggtcgcca gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa
    20401 atatgcaggg cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca
    20461 caatcccggt agaaattagc cagcaagtgg cgactatcga ggcaaaagca gaacacccca
    20521 tcacactacc tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat
    20581 tactcaaacg ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc
    20641 aagcgctaca caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc
    20701 cgaatatgga tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca
    20761 tctgggggct tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca
    20821 tgaacttatg tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt
    20881 tacaccaggt tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa
    20941 ataatacggc gaacgatcta caactgatgc aggagattct catgactttc cagcatgaaa
    21001 cgcataaaga tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc
    21061 atcagtgtat tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta
    21121 atattagcca tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc
    21181 agttgctgaa ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct
    21241 gtcagaaaaa tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag
    21301 cacatcttac atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt
    21361 tgccgctccc ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg
    21421 catcattatt cccccggctt caatcaacat atttcttgcc tgagtttgtg gcaatttaat
    21481 ggcctcagcc acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa
    21541 ccaaacttca gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg
    21601 gttttgtacg cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc
    21661 cagtgcctgg cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt
    21721 atcctgagta ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg
    21781 tggtgacttc cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc
    21841 atcaaacatc gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc
    21901 actcactatt ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac
    21961 gcgcactggc ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat
    22021 gatggtatcg taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga
    22081 agcatatatg aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc
    22141 cattgtacct gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa
    22201 tatactttta tcgtgatcat tctttaaatc aagaaccaca ctctctttgc catgattaat
    22261 aaaactgtaa tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc
    22321 atgacctggt ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc
    22381 gaaaggtcca ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt
    22441 gctttcatta tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc
    22501 ggcatttatg taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc
    22561 acaagggagc cgtgacgaca aaacccagta cgctcacagc caatgacgct gtaccagtgc
    22621 gggtgtagac attaaaccgg ctggcaataa tgattccgga gaatgccggc ggtaatgcgc
    22681 ctgccagtac catcatctgc aaatgttcgc tgttcaaatg acatgccata ccaa
//
LOCUS       DAFATA010000073        22667 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFATA010000073 DAFATA010000000
VERSION     DAFATA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.074"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(10..600)
                     /gene="slyD"
                     /locus_tag="LGJ20_003267"
     CDS             complement(10..600)
                     /gene="slyD"
                     /locus_tag="LGJ20_003267"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK2387288.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(695..895)
                     /locus_tag="LGJ20_003268"
     CDS             complement(695..895)
                     /locus_tag="LGJ20_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBK2387289.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(905..2710)
                     /gene="kefB"
                     /locus_tag="LGJ20_003269"
     CDS             complement(905..2710)
                     /gene="kefB"
                     /locus_tag="LGJ20_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBK2387290.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(2710..3261)
                     /gene="kefG"
                     /locus_tag="LGJ20_003270"
     CDS             complement(2710..3261)
                     /gene="kefG"
                     /locus_tag="LGJ20_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBK2387291.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            3392..5305
                     /locus_tag="LGJ20_003271"
     CDS             3392..5305
                     /locus_tag="LGJ20_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2387292.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            5305..6327
                     /locus_tag="LGJ20_003272"
     CDS             5305..6327
                     /locus_tag="LGJ20_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK2387293.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            6321..6539
                     /locus_tag="LGJ20_003273"
     CDS             6321..6539
                     /locus_tag="LGJ20_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBK2387294.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            6593..7462
                     /locus_tag="LGJ20_003274"
     CDS             6593..7462
                     /locus_tag="LGJ20_003274"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBK2387295.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(7517..7921)
                     /locus_tag="LGJ20_003275"
     CDS             complement(7517..7921)
                     /locus_tag="LGJ20_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBK2387296.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            8223..8855
                     /gene="crp"
                     /locus_tag="LGJ20_003276"
     CDS             8223..8855
                     /gene="crp"
                     /locus_tag="LGJ20_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBK2387297.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            8906..10996
                     /locus_tag="LGJ20_003277"
     CDS             8906..10996
                     /locus_tag="LGJ20_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK2387298.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(11063..12283)
                     /gene="argD/dapC"
                     /locus_tag="LGJ20_003278"
     CDS             complement(11063..12283)
                     /gene="argD/dapC"
                     /locus_tag="LGJ20_003278"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBK2387299.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVIGA"
     gene            complement(12369..12932)
                     /gene="pabA"
                     /locus_tag="LGJ20_003279"
     CDS             complement(12369..12932)
                     /gene="pabA"
                     /locus_tag="LGJ20_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBK2387300.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(12964..13566)
                     /locus_tag="LGJ20_003280"
     CDS             complement(12964..13566)
                     /locus_tag="LGJ20_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBK2387301.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(13556..13723)
                     /locus_tag="LGJ20_003281"
     CDS             complement(13556..13723)
                     /locus_tag="LGJ20_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBK2387302.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(13828..14400)
                     /gene="ppiA"
                     /locus_tag="LGJ20_003282"
                     /gene_synonym="rotA"
     CDS             complement(13828..14400)
                     /gene="ppiA"
                     /locus_tag="LGJ20_003282"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBK2387303.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            14671..15852
                     /gene="tsgA"
                     /locus_tag="LGJ20_003283"
     CDS             14671..15852
                     /gene="tsgA"
                     /locus_tag="LGJ20_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBK2387304.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            16114..18657
                     /gene="nirB"
                     /locus_tag="LGJ20_003284"
     CDS             16114..18657
                     /gene="nirB"
                     /locus_tag="LGJ20_003284"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBK2387305.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            18654..18980
                     /gene="nirD"
                     /locus_tag="LGJ20_003285"
     CDS             18654..18980
                     /gene="nirD"
                     /locus_tag="LGJ20_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBK2387306.1"
                     /translation="MNQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            19107..19913
                     /gene="nirC"
                     /locus_tag="LGJ20_003286"
     CDS             19107..19913
                     /gene="nirC"
                     /locus_tag="LGJ20_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBK2387307.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            19932..21305
                     /gene="cobA"
                     /locus_tag="LGJ20_003287"
     CDS             19932..21305
                     /gene="cobA"
                     /locus_tag="LGJ20_003287"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBK2387308.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            21560..21727
                     /locus_tag="LGJ20_003288"
     CDS             21560..21727
                     /locus_tag="LGJ20_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBK2387309.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCAPTAQ"
     gene            22022..>22667
                     /gene="frlA"
                     /locus_tag="LGJ20_003289"
     CDS             22022..>22667
                     /gene="frlA"
                     /locus_tag="LGJ20_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBK2387310.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASIC"
BASE COUNT         5457 a         6134 c         5767 g         5309 t
ORIGIN      
        1 ttttcggtat tagtggcaac cacaaccgcc gttgccttta ccgccacagc agccttcgcc
       61 accgtgttca tgaccgtgat cgtggccatg accgccgcag caaccgtcgt ggtcgtgatc
      121 gtggtgatga tcgtgcgcgc cgtgaacgtg gccatgagcc agttcttctt cggtcgcttc
      181 gcgaatcgcc acaacttcaa cgttgaattt caggttctga ccggccagca tgtggttacc
      241 atcaaccacg acgtgatcgt cttcaaccgc agtgatttca accggtaccg gaccctggtc
      301 agtttcagcc aggaaacgca tacctacctg cagttcatca acgcccataa atacgtcttt
      361 aggaacacgt tgcaccaggt tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc
      421 gacatcaaat ttgtcgccaa cttcatgacc ttccagcgcc gtttccaggc cagagatcag
      481 ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc ggagactcat caaccaacac
      541 accgtcttct gtacgtacct gataggccag gctgaccacc aggtcttttg ctactttcat
      601 gatatctcct gagcatggga agaatagtgg cgcagattgt agcggaattc tgcagccgtg
      661 tactcactag cttaaaaaaa ctcggcgcat atcgctagtc cggatgaaaa atcccgatca
      721 cttgctcatc tttgcgaacg tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc
      781 cgcacttaac acattcaaca atatcaatat tattttcgcg ccacatcgcc attgaatcct
      841 gcgcctgaca ggccgggcat tttgcgcccg caataaaacg ttttcggatt gccatcttta
      901 ccctctactc aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct
      961 ggaaaatctc ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acggtatcag
     1021 catgcatcgg gattaactcc cgcagcattc gcatatccag gcggcgaaaa tgcagttgcg
     1081 cccgctgtgc ctgatgcgga tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta
     1141 acgcactgga gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg
     1201 cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata
     1261 tttccaccag cttcatggtg tcttccggct cgttacaggt aatgacgata gactcagcgg
     1321 cctctgcacc cgcagaacgt aaaagatcga cctgcgtggc gtcgccgtaa taaactttgt
     1381 agccatattt acgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca
     1441 ttttatttgc catcagcaaa cgaccaatca cctgaccaaa acgcccgaag cccacgacaa
     1501 tgacctgggg tttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa
     1561 actggcggga tagccattta tccaccagct tcatcagcaa cggcgtggtc atcatggaaa
     1621 gcgtcaccgt caccagcaac agcgccatct ggtcgccctg gaataaacgt tgtgaagaag
     1681 cggtagaaaa gaggacaaag gcaaactcac cgccctgact caacacgcca gcaaactgca
     1741 tccgctctga gctacgcacg ccatacaatc gcgccagcag atacaacacg agaattttca
     1801 ccgccaccag cacaaccacg cttatcacta cccacaacag atgggtataa agcaccccga
     1861 gattgagcga catgccgaca gagataaaga acaaaccgag cagcaagcct ttgaagggat
     1921 cgatagccgt ttccagttca tggcgatatt cactttccgc cagcagcacg cccgcaataa
     1981 acgtaccgag cgccatcgac agccccagcg cgtccataaa caatgcggaa cccaacacca
     2041 acagcagcgt cgcggcggtg aacacttccc gcacgccaga agctgcaata aaacggaata
     2101 ccggacgcag taaatagcgc ccgccaatca gcatgccgac aaacgccagc accttcatgc
     2161 cgatcttcat ccagtcgaaa tgttcgtctg ccgaacccgc caacaacggc actaacgcca
     2221 gtgccgggat caccgccaga tcctgaaaca gcagaaccga aaacccgagc tgcccggatt
     2281 cgctgcgatt catccctttc tcacgcatca attgcaacgc cattgcagtt gaagacatcg
     2341 caaggccaat gccaccgaca accgccgcct gccaggcgaa atccgtcagc atcaataatc
     2401 ccgccagcaa cgccgcgctt aacagcacct gtgccgcgcc tacgccaaaa atcgaacgcc
     2461 gcagttgcca aagtttggag ggattcaact caagaccgat gataaacatc aggaatacca
     2521 cgccgagttc cgaaaagtgg aggatctcat cgacgtcgct aataaacccc agcccccatg
     2581 gaccaattgc aattcctgcc agcaaatatc ccaacacagc gccaataccc agccgcgatg
     2641 ccagcggcac cgcagccacc gccgcgaaga gaaacagcac tcctgcgagt aaaaaatcgg
     2701 aaccttccat cagcggcctc ctggagacag cggatttgcc agccagtcac cgtaggctct
     2761 ggcgtgactc gccagctcct gtgcgctttg ccgtctcgcc cagtaaataa tgatgggact
     2821 taaccaatgc atccggcaca tgcccgccgc cagttcaaag ggacgcagaa catcgctcat
     2881 cgggtagcga ttcaatgcgt cataacggta agcactttcc ggctcgccgg tggtaatcac
     2941 gctacgccag tactttcccg ccagttggtt tcctcccggc ccgctggcaa aaccacgact
     3001 taatacccgg tccagccact ctttcagtag cgccgggcag ctataggtat aaagaggatg
     3061 ctgaaagaca atcacctcgt gctcgcgcag taatgcctgc tcacggggga tatcaataaa
     3121 aaaatcggga tagtgcgcat aaaggtcgtg cacggtaaca ttgctgagct gcgtggccgg
     3181 tttaagcagt acccggtttg ccaccgagtc ctgagattcc ggatgggcat acagcagcaa
     3241 aactttcgct ggctgagaca tcatccccct cccggtatgg tttttgtgta tagtcgctgt
     3301 tttgggctac cattgcgccc ggtgcggcag ctcgcccata cattacatta tcataatgat
     3361 aagttaacat agtctgaaca tacggcgcct tatgattgtt ttctcctcgt tacaaattcg
     3421 tcgcggcgtg cgcgtcctgc tggataatgc caccgccacc atcaaccctg ggcagaaagt
     3481 cggcctggtg ggtaaaaacg gctgtggtaa atctaccctg ctggcattgc tgaaaaatga
     3541 aatcagcgcc gacggcggca gctacacctt tccgggaagc tggcaactgg cgtgggtgaa
     3601 tcaggaaacg ccggcgttac cgcaagcggc gctggaatat gtcattgacg gcgaccgtga
     3661 atatcgtcaa ctagaagcgc agctacacga cgccaacgaa cgtaacgacg ggcacgccat
     3721 tgcgaccatt catggcaagc tggatgctat tgacgcatgg agtattcgct cccgtgccgc
     3781 cagcctgctg cacggcctcg gtttcagcaa tgaacaactg gagcgcccgg taagtgattt
     3841 ctccggtggc tggcgtatgc gtcttaacct tgcccaggcg ctgatttgcc gttcagactt
     3901 gctgctgctc gacgaaccga ctaaccacct cgatctcgat gccgttatct ggctggaaaa
     3961 atggttgaag agctatcagg gcacgctgat cctgatctct cacgaccgcg acttcctcga
     4021 tccgatcgtt gataaaatta ttcatatcga acaacaaagc atgttcgagt acaccggcaa
     4081 ctacagttcg tttgaagtac agcgcgccac ccgtctggcg cagcaacaag cgatgtatga
     4141 aagccagcag gaacgcgtag cgcatctgca aagttatatc gaccgtttcc gtgccaaagc
     4201 caccaaagcg aagcaggccc agagccgcat taagatgctc gagcgtatgg agctgattgc
     4261 ccccgcgcac gtcgacaacc cgttccgctt tagcttccgc gcgccggaaa gcctgccaaa
     4321 tccgttactg aagatggaaa aagtcagcgc gggctatggc gatcgcatta ttctcgactc
     4381 gattaaactg aacctggtgc ccggctcgcg cattggtctg ttaggccgca acggcgcggg
     4441 taaatcgaca ttaatcaaac tgttagccgg tgaacttgcg ccagtcagcg gtgaaattgg
     4501 tctggcgaaa gggatcaagc tcggctactt cgcccagcat caacttgaat acctgcgcgc
     4561 cgacgaatca cctattcaac atctggcacg tttagcgccg caggagctgg aacaaaaact
     4621 gcgtgactac ctcggcggct ttggtttcca gggcgataaa gtaaccgaag aaacgcgccg
     4681 cttctcaggt ggggaaaaag cccgcctggt gctggcatta attgtctggc agcgtccgaa
     4741 tctgctgctg ctcgacgaac cgactaacca ccttgacctc gacatgcgtc aggcactcac
     4801 cgaagcatta atcgagtttg aaggcgcgct ggttgtcgtt tcgcacgacc gtcatttgct
     4861 gcgttccacc actgacgatc tctacctggt tcacgatcgt aaagtcgaac cgttcgacgg
     4921 cgatctggaa gattatcaac agtggttgag cgacgtacaa aagcaggaaa accagaccga
     4981 cgaagcgcca aaagagaacg cgaacagcgc ccaggcacgt aaagatcaga agcgtcggga
     5041 agcggagctg cgtgcgcaaa cccagccgct gcgtaaagag attgcccgtc tggaaaaaga
     5101 gatggagaag ctgaacgcgc aactggcgca ggcggaagag aaactcggcg acagcgaact
     5161 gtatgaccag agccgtaaag cggagttgac cgcctgcctg caacagcaag ccagcgccaa
     5221 atccggcctg gaagagtgcg aaatggcgtg gctggaagcc caggagcagc ttgagcagat
     5281 gctgctggaa ggccaaagca actgatggcg cagataacga cgaccgatgc caatgaattt
     5341 agcagcagtg ctgaattcac ccctatgcgc ggctttagca attgtcatct acaaactatg
     5401 ctgccgcgtt tgtttcgtcg tcaggtgaaa ttcaccccgt actggcagcg gctggagttg
     5461 cccgacggcg attttgtcga tctcgcgtgg agtgaagacc ccgcacaggc gaaacataaa
     5521 ccgcgtttag tggtgtttca cgggctggaa ggcagtctca atagccctta cgcccacggt
     5581 ctggttgagg cggcgcaaaa gcgcggctgg ctgggcgtgg tgatgcattt tcgcggatgc
     5641 agcggtgaac caaaccgtat gcaccgcatt taccattcgg gcgaaaccga agacgccagt
     5701 tggtttttac gctggctgca acgcgaattt ggtcatgcgc caacggctgc cgtcggctat
     5761 tcgctcggcg gtaatatgct ggcctgtttg ctggcaaagg aaggcaatga tctcccggtt
     5821 gatgcggcgg tgattgtctc tgcgccgttt atgctggaag cctgtagcta tcatatggaa
     5881 aagggctttt cccgcgttta tcagcgttac ttgctaaacc tgttaaaagc caatgccgca
     5941 cgcaagctgg cagcctaccc cggaacactg ccgattaatc tcgcgcagtt aaaatcggta
     6001 cgtcgcatcc gtgaatttga cgatctaatc accgccagaa ttcacggcta cgctgacgct
     6061 atcgactatt atcgtcagtg tagcgccatg ccgatgctga accggatcgc caaacctacg
     6121 ctgattattc acgccaaaga cgatccgttt atggatcatc aggtgatccc gaaaccggaa
     6181 agtctccccc cgcaggttga gtatcaactg actgaacatg gcggtcatgt tggctttatt
     6241 ggcggtacat tacttcatcc gcaaatgtgg ctggagtcac gcattcctga ctggttaaca
     6301 acgtatctgg aggcgaaatc atgttgattc cgtggcaaga cctctccccc gaaacgctgg
     6361 aaaatttaat tgaaagcttt gtgttacgtg aaggcaccga ttatggtgaa catgagcgta
     6421 cacttgaaca gaaagtcgcc gacgtcaaac gccagctaca gtgcggagaa gcggtgctgg
     6481 tatggtcgga gctgcacgaa acggtcaata ttatgccgcg cagtcagttt cgcgaataac
     6541 ctgcaaccgt cggcggacga gataataatg atagctaagt catggagtta ctatgtctgc
     6601 caaacatccg gtcattgcgg taacaggatc cagcggcgcg gggaccacca ccaccagcct
     6661 cgcgtttcgt aaaatattcg cgcagttaaa tctgcatgca gctgaggtgg aaggcgacag
     6721 ttttcaccgt tacacccgcc cggaaatgga catggcgatc cgcaaagcgc gcgacgccgg
     6781 gcggcatatc agctacttcg gccccgaggc taacgacttc ggcctgctgg aacaaacctt
     6841 cattgaatac ggtcagagcg gcaaagggaa atctcgcaaa tatctgcata cctacgacga
     6901 agccgtaccg tggaatcagg taccggggac attcaccccc tggcaacctt taccggaacc
     6961 cactgatgta ctgttttatg aaggtttaca cggcggcgta gtcacgccac agcataacgt
     7021 tgcgcagcat gtggacttac tggtcggcgt ggtgcctatc gttaaccttg agtggattca
     7081 aaaactgatc cgcgacacca gcgagcgcgg gcactcacga gaagcagtga tggactcggt
     7141 agtgcgttca atggaagatt atatcaacta catcacaccg cagttttccc gcacccatct
     7201 taacttccag cgcgttccca ccgtcgacac ttcaaacccg ttcgcggcaa aaggtatccc
     7261 gtcgctcgat gaaagctttg tggtgatcca ttttcgtaat ctggaaggga tcgatttccc
     7321 ctggctgctg gcgatgttgc aaggctcatt catttcccac atcaatacgt tagtggtacc
     7381 gggcggcaaa atgggtctgg caatggaatt aattatgctg ccgctggtgc aacgattgat
     7441 ggaaggaaag aaaatcgagt aactctgcta ttacgccgga taaaatacta tccggcttca
     7501 caacgggata gttaaatcac gcggcaacca cttcatacga gtgagtaata ttcaccgctt
     7561 tttccagcat caacgccact gaacaatatt tctcagcaga gagatcaacc gcacgcgcaa
     7621 ccgctgcgtc tttcaggtcg cgaccggtga cgataaaatg cagattaatg tgcgtaaaca
     7681 ggcgtggtgc ctcttcgcgg cgttcagagg tcaattttac ttcacaatcg accacatcct
     7741 gacgcccttt ttgcaggata gaaaccacat cgatggcact gcaacccccc gccgccatca
     7801 acaccatttc catcggactt ggtgctttat cgcctgagtt gccgtccatt aaaatctgat
     7861 gaccagaggc ggattcgccc agaaaagtta acccttcgac ccacttcact cgcgcttgca
     7921 tttttgctac tccactgcgt caattttcct gacagagtac gcgtactaac caaatcgcgc
     7981 aacggaaggc gacctgggtc atgctgaagc gagacaccag gagacacaaa gcgaaagcta
     8041 tgctaaaaca gtcaggatgc tacagtaata cattgatgta ctgcatgtat gcaaaggacg
     8101 tcacattacc gtgcagtaca gttgatagcc ccttcccagg tagcgggaag catatttcgg
     8161 caatccagag acagcggcgt tatctggctc tggagaaagc ttataacaga ggataaccgc
     8221 gcatggtgct tggcaaaccg caaacagacc cgactctcga atggttcttg tctcattgcc
     8281 acattcataa gtacccatcc aagagcacgc ttattcacca gggtgaaaaa gcggaaacgc
     8341 tgtactacat cgttaaaggc tctgtggcag tgctgatcaa agacgaagag ggtaaagaaa
     8401 tgatcctctc ctatctgaat cagggtgatt ttattggcga actgggcctg tttgaagagg
     8461 gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct gaaatttcgt
     8521 acaaaaaatt tcgccaattg attcaggtaa acccggacat tctgatgcgt ctgtctgcac
     8581 agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg caacctggcg ttcctcgacg
     8641 tgacgggccg cattgcacag actctgctga atctggcaaa acaaccagac gctatgactc
     8701 acccggacgg tatgcaaatc aaaattaccc gtcaggaaat tggtcagatt gtcggctgtt
     8761 ctcgtgaaac cgtgggacgc attctgaaga tgcttgaaga tcagaacctg atctccgcac
     8821 acggtaaaac catcgtcgtt tacggcactc gttaatcccg tcggagtggc gcgttacctg
     8881 gtagcgcgcc attttgtttc ccccgatgtg gcgcagactg atttatcacc ccgatatcaa
     8941 ctatgcactt cgacaaacgc tggtgctatg tttgcccgtg gccgttgggt taatgcttgg
     9001 cgaattacga ttcggtctgc tcttctcact cgttcctgcc tgttgcaata ttgcgggcct
     9061 tgatacgcct cataaacgtt ttttcaaacg cttaatcatt ggtgcgtcgc tgtttgccac
     9121 ctgtagcttg ctgacacagc tactactggc aaaagatgtt cctctgccct ttttgctgac
     9181 cggattaacg ctggtacttg gcgtcactgc tgagctgggg ccattgcacg ctaaattgct
     9241 tcccgcatcg ctgctcgccg ccatttttac cctcagtctg gcgggatata tgccggtctg
     9301 ggaaccattg ctcatctatg cgttgggcac tctctggtac ggattgttta actggttttg
     9361 gttctggatc tggcgcgaac aaccgctgcg cgagtcacta agtctgctgt accgtgaact
     9421 ggcagattat tgtgaagcca aatacagcct gcttacccag cacaccgacc ctgaaaaagc
     9481 gctgccgccg ctgctggtgc gccagcaaaa agcggtcgat ctaattaccc agtgctatca
     9541 gcaaatgcat atgctttccg cgcaaaataa tactgactac aagcggatgc tgcgtatttt
     9601 ccaggaggcg ctggatttac aggaacatat ttcggtcagt ttgcatcagc cggaagaggt
     9661 gcaaaaactg gtcgagcgta gccatgcgga agaagttatc cgctggaatg cgcaaaccgt
     9721 cgccgctcgc ctgcgcgtgc tggctgatga cattctttac catcgcctgc caacgcgttt
     9781 tacgatggaa aagcaaattg gcgcactgga aaaaatcgcc cgccagcatc cggataatcc
     9841 ggttgggcaa ttctgctatt ggcacttcag ccgcattgcc cgcgtgctgc gcacccaaaa
     9901 accgctctat gcccgtgact tactggccga taaacagcgg cgaatgccgt tacttccggc
     9961 gctgaaaagt tatctgtcac taaagtctcc ggcgctacgc aatgccggac gactcagtgt
    10021 gatgttaagc gttgccagcc tgatgggcac cgcgctgcat ctgccgaagt cgtactggat
    10081 cctgatgacg gtattgctgg tgacgcaaaa tggctatggc gcaacccgtt tgaggattgt
    10141 gaatcgctcc gtgggaaccg tggtcgggtt aatcattgcg ggcgtggcgc tgcactttaa
    10201 aattcccgaa ggttacaccc tgaccttgat gctgattacc accctcgcca gctacctgat
    10261 attgcgcaaa aactacggct gggcgacggt cggttttacc attaccgcag tgtataccct
    10321 gcaactgttg tggctgaacg gtgagcagta catccttcca cgtcttatcg ataccattat
    10381 tggttgtttg attgctttcg gtggtaccgt ctggctgtgg ccgcagtggc agagcgggtt
    10441 attgcgtaaa aacgcccatg atgctttaga agcctatcag gaagcgattc gcttgattct
    10501 gagcgaggac ccgcaaccta ccccactggc ctggcagcga atgcgggtaa atcaggcgca
    10561 taacactctg tataactcat tgaatcaggc gatgcaggaa ccagcgttca acagccatta
    10621 tctggcagat atgaaactgt gggtaacgca cagccagttt attgttgagc atattaatgc
    10681 catgaccacg ctggcgcggg aacaccgggc attgccacct gaactggcac aagagtattt
    10741 acagtcttgt gaaatcgcca ttcagcgttg tcagcagcgg ctggaatatg acgaaccggg
    10801 gagttctggc gatgccaata tcatggatgc gccggagatg cagccgcacg aaggcgcggc
    10861 aggtacgctg gagcagcatt tacagcgggt tattggtcat ctgaacacca tgcacaccat
    10921 ttcgtcgatg gcatggcgtc agcgaccgca tcacgggatt tggctgagtc gcaagttgcg
    10981 ggattcgaag gcgtaatgca ggctaaacca tcggataagg cattcacgcc gcatccgaca
    11041 tcttttgcct gatgcttcgt atttacgccc caatcacctt agccaccgcg tgggcgaaac
    11101 gttgcatccc ttcatcgata tccgcatctt ccaccaccag cgacggtgca aaacgcatca
    11161 catccggtcc ggcattcagc accattacgc cagcctctgc gcccgcatac aggaaatcac
    11221 gcgcccgacc tttgaactgt ggcttaagct ccgcgccaat caacagcccc ataccgcgaa
    11281 tatcgctaaa cacatcgtac tgctgatcga tcttctgcaa atggtcaaca aaacgctggc
    11341 gtttcgcctg aatgccttcc agcacttcag gggtattgat gatatcaaac gccgcgcccg
    11401 ctactgcaca ggccagagga ttaccgccgt aggtggaacc gtgagaacca ggatgaaacg
    11461 cagaagcaat ttccgccgtg gtcagcatgg cgctaatcgg gaagccgccg cctaacgctt
    11521 tcgcagaggt cagaatatcc ggcgtcacac cgtagtgcat gtaagcaaac aaatcgccag
    11581 tccgccccat cccgcactgc acttcatcaa acaccaataa tgcctgatgt tgatcgcaca
    11641 gctcgcgcaa gccctgcaaa aactctggcg tcgcagccgt cacaccgccc tcgccctgga
    11701 tcggctcaac caccaccgca caggtgtgat catccatcac cgctttcact gcgtggagat
    11761 cgttaaaggg aacgtggatg atgtctgccg gttttggccc aaagccgtcg gaatattttg
    11821 gctgcccgcc caccgagacg gtaaacagcg agcgaccatg aaaagcgtta tggaaggcaa
    11881 taattttggt tttgaacggg ctatgacgca cacaggcata atggcgtgcc agtttaaagg
    11941 cggtttcgtt agcttccgtg ccggagttca taaacaccac gcgttcggca aacgtcgcct
    12001 caatcagttt gcgcccaaga cgcagcgccg gttcattggt gaaaacgtta ctgatatgcc
    12061 acagagtttc gccctgggtt tttaacgcat tcaccagcgc aggatggcaa tggcccaacg
    12121 ccgtaactgc aatgccaccc gcgaaatcga catactcctt gccttgctga tcccagattc
    12181 ggctgccctg accttttacc ggaatgaact ctgccggagc ataaatcggc aggatcactt
    12241 catcgaaagt cgcccgtgta attgctgttt gttcaattgc catctcatga tcaccctgtt
    12301 acgcataaat aaatgtgaaa ttataatcac aaaatatgca taaaaaatca ctgaatggca
    12361 atcagaaatc agcgatgcag aaaattagcc agcagttgat gtccttgttc gctaagaata
    12421 ctttctggat ggaattgcac accttccaga tcccactggc gatggcgaat ccccataatc
    12481 tcgcgggttt cgctccaggc cgtcacgtca aagcacgctg gtaatgagtc aggttccacc
    12541 accagcgaat ggtagcgtgt cacggtgagt ggatttgcca gcccccgaaa tacgccctca
    12601 ccgttatgtg taatcggcga ggttttgccg tgcataacct ttgcggcgcg cacaacttta
    12661 ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga cgccaagaat cggcaagcgc
    12721 ccggcatagt ggcgaataac gtcaagggag atcccggctt catctggcgt acaggggcca
    12781 ggtgagatga caattttttg tggtttaagg gcgtcgatat ccgccagcgt caacgcatcg
    12841 ttgcgcttaa ccagcacatc cgcccccagt tcacaaaagt actggtagag gttccaggta
    12901 aaagaatcgt agttatctat aagcaggatc atggcggctc cggtacgaaa gaaccgcgct
    12961 attttactca gattccccgg cttcgcttac cactttgcta aatatcatct gcagcgcggt
    13021 gagatccccc attgctccac tctgatttgc ctgattccag gcctcttttt cgataccctg
    13081 ccagctcagt tgatatccgg catgaatcgc cagttgctcg aagaagatcc gctgtgccag
    13141 accacttccc acccggaagg gatgcagcac gttgatttca caatagtaat gcgccagccg
    13201 ctcgacgaac ttcgctttct ccaggccaac cagataacct tcttcctcca gatcctgcat
    13261 cagggcattg ccctcttttt cgatataagc aaagtggcag aacggcgtat cgccctgata
    13321 aatatcaact tcacgcagtt gccctgccca gtcgaaaata tcctggtaca gctggcgatg
    13381 gatagtgcgc aaatgcggta aaccgcgcac cagcggacca agctcaatgg tcgcagcacg
    13441 cagcgccgtc atttcgtaag cggcctgttc cagccgctgc tgctggcgga tgttcagccg
    13501 gttacgcatg atatcaaggc caggataaag atacggatcg cgcccttcgc cgaatttatc
    13561 gctcatagtg actcctcagc tcttcaaggc gcgctaaagc ctctgcggca gtaagagtga
    13621 ctaaaggcat ctcgacgcct tcaagacggc gactggcctg aaaattacga ttacgctgaa
    13681 gctcccagag acgggacttt tgcttatcgg tgagtttctt cacttgatgc ctccctgaac
    13741 gtgtccattt gccacaagta taagcggcaa atccgggtta cgccgggaga gcaaaagcgc
    13801 gggcaagatt gcccgcgcga gaaatcatta cggcaggact ttagcggaaa ggataactac
    13861 cggttttgac ggcacattct ggtacggacc aacgtcatga gtcggcacct gggaaatctt
    13921 atcggcaacg tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg
    13981 accatggtca aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc
    14041 tttgtcggcg gtacgcgcca tcgcgatggt accacgcgtg ttgcgcaggc cattatcggc
    14101 ttcatttttg attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc
    14161 ctgaatcata aagccaggaa tgacgcggtg aaaggtagtg ttgttataaa aaccgctgtt
    14221 cacataatcg acaaagttct gcacagacac tggcgctttt tgtttatcca gctccagttc
    14281 gatgttacca gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc
    14341 gggagaaaga gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat
    14401 gagatttcct taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc
    14461 aagaacaagg ccatcccttt acctaatttt acgtatctga aatatatgta acctgacgat
    14521 cactttttat tccgtgatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat
    14581 aaaacattta aacagatcac aaaatcacct aaaatcgccc gctcgcggca tatcccgcta
    14641 tgccatttta tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca
    14701 tggattagct ttctctccta cgcactgact ggtgcgttgg ttattgtcac cgggatggtg
    14761 atgggaaata tcgccgatta tttcaatctg cctgtttcca gtatgagtaa caccttcacc
    14821 ttcctcaacg ccggcatttt aatctctatc ttcctcaacg cctggctgat ggaaatcgtc
    14881 ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg
    14941 atgttcagcc acagcctggc gctgttctcg gcggcgatgt tcattctcgg ggtggtcagc
    15001 ggcatcacca tgtcgattgg tacattcctg gtaacacaaa tgtatgaagg gcgtcagcgc
    15061 ggttcccgcc tgttatttac cgactccttc ttcagtatgg ctgggatgat tttcccaatg
    15121 atcgccgcgt ttctactggc gcgcagcatt gagtggtact gggtttatgc ctgcatcggg
    15181 ctggtgtatg tcgctatttt tattctgacc ttcggctgtg agttcccggc gctgggcaaa
    15241 catgcgccaa aaacagatgc tccggtagag aaagaaaagt gggggatcgg cgtactgttt
    15301 ctctctgttg cggcgctgtg ctacatcctt ggtcagttag gttttatctc ctgggtgcct
    15361 gagtatgcca aaggcctggg catgagcctg aacgacgcgg gcacgctggt gagtaacttc
    15421 tggatgtcat acatggtcgg catgtgggcg ttcagcttta ttcttcgctt ctttgatttg
    15481 caacgtattc tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc
    15541 ggaacaccag cacatatggc gtggtcaatt ctcgctctgg gcttcttctc cagcgcgatc
    15601 tataccacca tcatcaccct gggttcacag cagaccaaag taccgtcgcc aaaactggtt
    15661 aactttgtcc tgacctgcgg aaccatcggt actatgttga cctttgtggt taccggcccg
    15721 attgttgaac atagcggtcc gcaggcggca ctgcttacag caaacggtct gtacgctgtc
    15781 gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg
    15841 acctctcatt aattgctaat gccggacggc actatcgtcg tccggccttt tcctctcttc
    15901 ccccgctacg tgcatctatt tctataaacc cgttcatttt gtccattttt tgcacaaaca
    15961 tgaaatgtca gacaattccg tgacttaaga aaatttatac aaatcagcaa tatacccctt
    16021 aaggagtata taaaggtgaa tttgatttac atcaataagc ggggttgctg aatcgttaag
    16081 gtaggcggta atagaaaaga aatcgaggca aaaatgagca aagtcagact cgcaattatc
    16141 ggtaacggta tggtcggcca tcgctttatc gaagatcttc ttgataaatc tgatgcggcc
    16201 aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg cttatgaccg cgtacacctc
    16261 tcgtcttact tctctcacca caccgccgaa gagctgtcgc tggtgcgcga aggcttctac
    16321 gagaaacacg gcatcaaagt tctggtcggc gaacgtgcta tcaccatcaa ccgtcaggag
    16381 aaggtgattc actccagcgc cgggcgcacc gttttttatg acaagctgat catggcgacc
    16441 ggttcctacc cgtggatccc gccaatcaaa ggttctgata ctcaggactg ctttgtctat
    16501 cgcaccattg aagacctcaa cgccattgaa tcctgtgccc gtcgcagcaa acgcggtgcc
    16561 gttgttggtg gcggcctgtt aggtctggaa gccgctggcg cgctgaaaaa cttaggtatt
    16621 gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg aacagcttga tcagatgggt
    16681 ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc gcgttcacac cagcaaaaac
    16741 acccttgaga ttgtgcagga aggtgtcgaa gcgcgtaaaa ccatgcgttt tgccgacggc
    16801 agcgaactgg aagtcgactt tatcgtcttc tctaccggta tccgtccgcg cgataagctg
    16861 gcaacccagt gtggtctgga cgtagctccg cgtgggggta ttgtcattaa tgattcctgc
    16921 cagacttccg atccggatat ctacgccatc ggtgaatgcg caagctggaa caaccgtgta
    16981 tttggtctgg tagcaccagg ctacaaaatg gcgcaggtcg ccgttgacca tattctcggt
    17041 agcgaaaacg cctttgaagg tgctgacctt agcgccaagc tgaaactgct gggcgtagac
    17101 gtaggcggta ttggtgatgc gcacggtcgc acgcctggcg cacgtagcta cgtttacctc
    17161 gacgaaagca aagagatcta caaacgcctt attgtcagcg aagacaacaa aaccctgctc
    17221 ggtgcggtac tggtgggcga taccagcgac tacggcaacc tgctgcaact ggtgctgaat
    17281 gctatcgagc tgccggaaaa cccggattct ctgattctgc cggcgcactc gggtagcggt
    17341 aagccgtcta tcggtgttga taaactgccg gacagcgcgc aaatctgctc ctgcttcgac
    17401 gtcaccaaag gtgatctgat tgctgccatc aacaaaggct gccacaccgt tgcggcgctg
    17461 aaagccgaaa ccaaagcggg tactggctgc ggtggctgta tcccgctggt cactcaggta
    17521 ctgaacgcgg aactggcgaa acagggcatc gaagttaaca acaacctgtg cgaacacttt
    17581 gcttattcgc gtcaggaact gttccatttg atccgcgttg aaggcattaa aaccttcgaa
    17641 gaactgctgg cgaaacacgg caaaggttac ggttgtgaag tttgtaaacc gaccgtcggt
    17701 tcgctgctgg cctcctgctg gaacgaatac attctgaagc cggaacatac tccgctgcag
    17761 gattcaaacg acaacttcct cgctaacatc cagaaagacg gcacctactc ggtgatcccg
    17821 cgttctccgg gcggtgaaat caccccggaa gggctgatgg cagtaggtcg tatcgcgcgt
    17881 gaatttaatc tctacaccaa aatcaccggc tctcagcgcc tggcgatgtt tggcgcacag
    17941 aaagacgatc tgccggagat ctggcgtcag ctgattgaag ctggcttcga aaccggtcat
    18001 gcctatgcga aagcactacg tatggcgaaa acctgcgtgg gtagcacctg gtgccgctac
    18061 ggcgttggcg acagcgtcgg cctcggcgtg gaactggaaa accgctacaa aggcatccgt
    18121 acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc gtgaatgttc agaagctcag
    18181 ggtaaagacg tgggtattat cgccactgaa aaaggctgga acctgtatgt ttgcggtaac
    18241 ggcggcatga aaccgcgtca tgcggatctg ctggcggcgg atatcgatcg cgaaacgctg
    18301 atcaaatatc tcgaccgctt catgatgttc tacatccgta ccgccgacaa actgacgcgt
    18361 accgcaccgt ggttagaaaa cctcgaaggc ggcattgatt acctgaaagc ggtgatcatt
    18421 gacgacaaac tggggctgaa cgcacatctg gaagaagaga tggcgcgcct gcgtgaagcg
    18481 gtagtgtgtg agtggactga aacggtcaat acgccgtctg cgcagactcg cttcaaacac
    18541 ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg tgccagagcg cgaacagcac
    18601 cgtccggcaa cgccgtatga acgtatcccg gtaactctgg tggaggacaa cgcatgaacc
    18661 agtggaaaga catctgcaaa atcgatgaca tcctgcctga aaccggcgtc tgcgcgctgt
    18721 taggtgacga gcaggtagca attttccgcc cgtatcacag cgaccaggtg tttgcgatca
    18781 gcaacattga tccgttcttc gagtccagcg tgctgtcacg cgggctgatt gcggaacacc
    18841 agggcgagct gtgggtcgcc agcccgctga aaaaacagcg ttttcgctta agtgatggct
    18901 tgtgcatgga agacgaacag ttttccgtca aacattacga agcacgagtg aaagacggcg
    18961 tggtgcagct gcgcggttaa tgttttaacg ggaggcgcaa tgcctcccct ttttgcatgg
    19021 tcctgtaatg accttcggta tattgcagga cattttttaa actttttgtt ttattttttt
    19081 gtttttattt tttaaaggat aatcaaatgt ttacagacac tattaataag tgtgcggcta
    19141 acgctgcgcg cattgcacgc ctgtcggcaa ataacccgct cggcttttgg gtcagctccg
    19201 ccatggcggg cgcgtatgtg ggtcttggga tcatcttgat tttcacgctc ggtaatttgc
    19261 tcgacccgtc cgtacgtccg ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg
    19321 tgattatcgc cggttctgaa ctgttcaccg gacacaccat gttcctcacc tttggggtaa
    19381 aagcgggcag catcagccac gggcaaatgt gggcaatcct gccgcaaacc tggctgggta
    19441 acctggtcgg ttccgtcttc gttgccatgc tctatagctg gggtggcggt agcctgctgc
    19501 cggtagatac cagcatcgtt cactccgtcg cgctggctaa aaccactgca ccggcaatgg
    19561 tactgttctt caaaggtgca ttgtgtaact ggctggtttg tctggcaatc tggatggcgc
    19621 tgcgcactga aggggcggcg aaatttatcg ctatctggtg gtgtctgctg gcatttatcg
    19681 cgtccggcta cgagcactcc atcgctaaca tgacgctgtt cgcgctctcc tggttcggca
    19741 accacagcga agcctacacg ctggcgggta ttggtcataa cctgctgtgg gtgacgctgg
    19801 gtaatacttt atcaggtgcc gtattcatgg gattgggtta ttggtatgct acgccgaaag
    19861 cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac ggctgccggt taattactaa
    19921 ggggttttta cgtggatcat ttgcctatat tttgccaatt acgcgatcgc gactgtctga
    19981 ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt gctgttagac gcaggcgctc
    20041 gcttaacggt gaatgcatta gcgtttattc cacagttcac cgcatgggca gatgcaggca
    20101 tgttaaccct cgtcgaaggg ccatttgatg aaagccttct cgacacctgc tggctggcga
    20161 ttgcagcgac ggatgatgac gcgcttaacc agcgcgtcag cgaagccgct gaagctcgtc
    20221 gcatcttctg taacgtggtc gatgcgccga aagctgccag cttcattatg ccgtcgatta
    20281 ttgaccgctc accgctgatg gtggcggttt cctctggcgg tacttctccg gttctggcgc
    20341 gcctgttgcg cgaaaaactc gaatcactgc tgccgctgca tctgggccag gtagcgaaat
    20401 acgccgggca gttacgcggt cgggtaaaac aacagttcgc cacgatgggt gaacgccgcc
    20461 gcttctggga aaaactgttc gttaacgacc gtctggcgca gtcgctggcg aacaacgatc
    20521 agaaagccat tactgaaacc accgaacagt taatcaacga accgctcgac catcgcggtg
    20581 aagtggtgct ggttggtgca ggtccgggcg atgccgggct gctgacgctg aaaggactgc
    20641 aacaaattca gcaggcagat gtggtggtct acgaccgtct ggtttctgac gatattatga
    20701 atctggtacg ccgcgatgct gatcgcgttt tcgtcggcaa acgcgcggga taccactgtg
    20761 taccgcagga agagattaac cagatcctgc tgcgggaagc gcaaaaaggc aaacgcgtgg
    20821 tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg tggcgaagag ctggaaacac
    20881 tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat taccgcagct tctggttgct
    20941 ctgcctattc gggtattccg ctcacgcatc gcgattatgc ccagagcgtg cgcttaatta
    21001 ccggacactt aaaaaccggt ggcgagctgg actgggaaaa cctggcggca gaaaaacaga
    21061 cgctggtgtt ctatatgggg ctgaatcagg ctgcgactat tcagcaaaag ctgattgaac
    21121 acggtatgcc tggcgaaatg ccggtagcaa ttgtcgaaaa cggaacagca gtcacgcagc
    21181 gcgtgattga cggtacgctc acacaactgg gcgaactggc gcagcaaatg aacagtccat
    21241 cgctaattat tattggtcgg gttgttggcc tgcgcgataa actgaactgg ttctccaacc
    21301 attaatttaa tcagggccag agaattctgg cctttttcgt ttacaactct ctttatatat
    21361 tcagcagata aaaatataac tcattaacaa aaatcatttg tttttgcccc gttatattca
    21421 ccgttacagc gacttacaaa attaagtcat tcactcactg acgccgatta aatattcctg
    21481 tatcgttctt aatgagcagt ctacttatat tcatataatc aatgaatatt aatcaatata
    21541 taatacatag ggaatgtaaa tgaacaaatt tattaaagtt gcactggtag gtgcggtact
    21601 ggctacgtta actgcatgta ctggtcatat tgaaaaccgt gataagaact gctcttacga
    21661 ctacctgctg cacccggcaa tttctatttc taaaatcatt ggcggttgcg ctcctactgc
    21721 acagtaagtc tctgacagaa atcggctaac accggtttaa ttgcccgacg ccctctggat
    21781 tttccgaggg cgtatttgtt tctatgtcct tcttgccccc gcccgtaaaa atattttcac
    21841 tttaaattca atttgataaa tacatccatt agccacagcg cgatcctgtg cgaaattttg
    21901 tgatcttcct ccacattaca taacatcata tgttgttata ttcatcatgc attgtcatgt
    21961 taccttttaa atgactgcaa actctcccct acaacgtatt ggacaagaaa aaggtatcgc
    22021 tatgggaagc caggaactcc aacgcaagct cggattttgg gccgttcttg caatcgccgt
    22081 cgggacaacc gtcggctccg gtatttttgt atctgtgggt gaagtggcaa aagcagcggg
    22141 cacgccgtgg cttacggtgc tcgcgtttgt cattggcggg ttaattgtga tcccgcaaat
    22201 gtgcgtctat gcggaactat ccaccgctta tccggaaaat ggcgcagatt atgtttatct
    22261 gaaaaatgcc ggaagccgac cgctggcttt cctctccggc tgggccagct tctgggccaa
    22321 cgatgcgccg tcattgtcga ttatggcgct ggcgattgtc agcaatcttg gctttttaac
    22381 gcctatcgat ccgttgctcg gtaaatttat cgccgccgga ttaattatcg cctttatgtt
    22441 gctacacctg cgctccgttg aaggcggcgc agcgtttcag acgctaatta ccatcgccaa
    22501 aattatcccg ttcactatcg tcattggcct tgggatcttc tggtttaaag cggagaattt
    22561 tgccgcccct accaccactg cgattggcgc aacgggcagc tttatggcgc tgctggcggg
    22621 gatctctgcc accagttggt cgtataccgg catggcctct atctgtt
//
LOCUS       DAFATA010000074        22206 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFATA010000074 DAFATA010000000
VERSION     DAFATA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.075"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..521)
                     /locus_tag="LGJ20_003290"
     CDS             complement(<1..521)
                     /locus_tag="LGJ20_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HBK2387311.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVY"
     gene            complement(518..1597)
                     /gene="rffT"
                     /locus_tag="LGJ20_003291"
                     /gene_synonym="wecF"
     CDS             complement(518..1597)
                     /gene="rffT"
                     /locus_tag="LGJ20_003291"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBK2387312.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(1594..2844)
                     /gene="wzxE"
                     /locus_tag="LGJ20_003292"
     CDS             complement(1594..2844)
                     /gene="wzxE"
                     /locus_tag="LGJ20_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBK2387313.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKATLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(2846..3976)
                     /gene="rffA"
                     /locus_tag="LGJ20_003293"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(2846..3976)
                     /gene="rffA"
                     /locus_tag="LGJ20_003293"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBK2387314.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(3981..4655)
                     /gene="rffC"
                     /locus_tag="LGJ20_003294"
                     /gene_synonym="wecD"
     CDS             complement(3981..4655)
                     /gene="rffC"
                     /locus_tag="LGJ20_003294"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBK2387315.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(4633..5514)
                     /gene="rfbA"
                     /locus_tag="LGJ20_003295"
     CDS             complement(4633..5514)
                     /gene="rfbA"
                     /locus_tag="LGJ20_003295"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK2387316.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(5533..6600)
                     /gene="rffG"
                     /locus_tag="LGJ20_003296"
     CDS             complement(5533..6600)
                     /gene="rffG"
                     /locus_tag="LGJ20_003296"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK2387317.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(6600..7862)
                     /gene="wecC"
                     /locus_tag="LGJ20_003297"
     CDS             complement(6600..7862)
                     /gene="wecC"
                     /locus_tag="LGJ20_003297"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBK2387318.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(7859..8989)
                     /gene="wecB"
                     /locus_tag="LGJ20_003298"
     CDS             complement(7859..8989)
                     /gene="wecB"
                     /locus_tag="LGJ20_003298"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBK2387319.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(9045..10091)
                     /gene="wzzE"
                     /locus_tag="LGJ20_003299"
     CDS             complement(9045..10091)
                     /gene="wzzE"
                     /locus_tag="LGJ20_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBK2387320.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(10103..11206)
                     /gene="wecA"
                     /locus_tag="LGJ20_003300"
     CDS             complement(10103..11206)
                     /gene="wecA"
                     /locus_tag="LGJ20_003300"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBK2387321.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(11446..12705)
                     /gene="rho"
                     /locus_tag="LGJ20_003301"
     CDS             complement(11446..12705)
                     /gene="rho"
                     /locus_tag="LGJ20_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK2387322.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(12790..12891)
                     /gene="rhoL"
                     /locus_tag="LGJ20_003302"
     CDS             complement(12790..12891)
                     /gene="rhoL"
                     /locus_tag="LGJ20_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBK2387323.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(13032..13361)
                     /gene="trxA"
                     /locus_tag="LGJ20_003303"
     CDS             complement(13032..13361)
                     /gene="trxA"
                     /locus_tag="LGJ20_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBK2387324.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            13492..14757
                     /gene="rhlB"
                     /locus_tag="LGJ20_003304"
     CDS             13492..14757
                     /gene="rhlB"
                     /locus_tag="LGJ20_003304"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBK2387325.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            14891..16375
                     /gene="gppA"
                     /locus_tag="LGJ20_003305"
     CDS             14891..16375
                     /gene="gppA"
                     /locus_tag="LGJ20_003305"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBK2387326.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(16422..18443)
                     /gene="rep"
                     /locus_tag="LGJ20_003306"
     CDS             complement(16422..18443)
                     /gene="rep"
                     /locus_tag="LGJ20_003306"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBK2387327.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            18660..19109
                     /locus_tag="LGJ20_003307"
     CDS             18660..19109
                     /locus_tag="LGJ20_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK2387328.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            19308..19589
                     /gene="ppiC"
                     /locus_tag="LGJ20_003308"
     CDS             19308..19589
                     /gene="ppiC"
                     /locus_tag="LGJ20_003308"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBK2387329.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(19676..21151)
                     /gene="ilvC"
                     /locus_tag="LGJ20_003309"
     CDS             complement(19676..21151)
                     /gene="ilvC"
                     /locus_tag="LGJ20_003309"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBK2387330.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            21301..22194
                     /gene="ilvY"
                     /locus_tag="LGJ20_003310"
     CDS             21301..22194
                     /gene="ilvY"
                     /locus_tag="LGJ20_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBK2387331.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
BASE COUNT         5433 a         6054 c         5585 g         5134 t
ORIGIN      
        1 aagtagacca ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg
       61 gagacttcac tggagaagat ctgactgtag gagttaagcc ggaacagcaa aaagccgttg
      121 tgcatgaaga agatgccgac gcttaccagc gcgatgccca tcaggatcac ccacgtaaga
      181 ttggtctcca cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg
      241 cgtaggcggg ttttgtaggt gacatagtaa accgcgtaga agcagcccgc agaaagcaac
      301 gcctgcaaca agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg
      361 gtcagcggga agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag
      421 ttaaagcgca cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc
      481 agccagacaa caaacaggcc actgaattgc agcagactca tgcgacctcc ccggcggcaa
      541 tcgccaacgc ccgctgccag ccttgtagat agttagggct aaagaaggca atggtgtttt
      601 tatccaccga cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg
      661 tagtaaacag caccggcaaa tgctgttccg tcatatcctg ccagaacgga ttttcccggt
      721 taagcacaca aggaatgccc gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc
      781 gggcaaaaat aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt
      841 ccagtttttc gctcaaaatt tgcagatttt cttcgctgaa taactccagc cccgcctgac
      901 gaacttcctc aatgtacgct tcgttattag gcggatatcc catcggcacc accactttta
      961 ccgtatcgcc aaattgctga tgaacggcgc acaaggcagc aatatggtca ttgctgcggt
     1021 caccggagtt ccccaccaga atggtcattt tcccttcacg ttgccgatcg ttcgccatcg
     1081 tattgagaga agggtccatc cgcgtcggga agtacagcag ttcgccccgc acctttggat
     1141 gcgttttggc aaaaaagctc aaatcgccgc gggtggcaaa tacacagccg actcgctttt
     1201 gcgccaggcg acgtagtggg taaaaaagct tatatctcaa gccactggaa agctcataca
     1261 ggtctgcccc ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag
     1321 ccagccacag tgtgggattg aactgaccgt ggaagaaaaa acgctgctga cggttagctt
     1381 ttgctttcgc gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa
     1441 gcgccggaca gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt
     1501 gctcgctcgt cgcagccagc gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag
     1561 ggatatccga tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa
     1621 cacgccacaa caaagagaaa aatagacgat ataagttgcc atatatgcct gcgccgcgcc
     1681 cagtgcacca tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct
     1741 gatttccgcc agaatataaa accgcaatgt cgctttggcg atcaccagat aaccaaagac
     1801 ataagcgccc acttttaaca catcacccac taactgccag gcaaagagat cgcgcatagc
     1861 ggtaaattta ttcgacaaca gcagccagat agcaaaatca cgcagcagcc agacggtaaa
     1921 actcgccgcc gccactgccg gtaagacgaa tttcagcgat ttaaccactt cccgggtgat
     1981 atcgcgcttt tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt
     2041 aataaattgc aggtaggcat cggaaatact gctcacccct tgccagatcc ccacttcatc
     2101 ccagctatac tgcgccgcga gcagtttacg catcatgatg taagcaacag gcaaggtcac
     2161 cgacgtaatc aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca
     2221 gctgggtttc agatagctta acgggatgac accacgtttg atcaacatga tggcggcagg
     2281 aattaccacc agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgcccaa
     2341 acggtaactg acgtagtacg cgagaacgcc aatcaagctg ccgacaatca gcgataacgc
     2401 attacctgcg gcatcgcgaa agcctttcat cagcgccagt aacaggttgc cccaggcgat
     2461 ccccatttgc accagcgcca ctaaacgcac cagcccctga tagtcggtat taccaaacaa
     2521 tccctggctg attggcgcag ctgccagcac aaaaaccagc gccatcagcg tagagaaacc
     2581 aagtaccatc gctgatgaag tgccgaccac gcggcgcagc tgttgcggat tatcatggta
     2641 ctgggcaacg tatttggtta caccgttaaa gatgccagct ccggcaagca cgccgagcac
     2701 ggtaatcaac tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacacagc
     2761 cagcaactta ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca
     2821 caaggacgct tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg
     2881 tacgctgatt gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcgctct
     2941 ctttggtggt gtagcgatct tcaccgtgga actcaccaaa gcgttccccc gcagggcaac
     3001 cgtgcagcgg aatgtaatgg aacaccgcca tgatttccgc ttctttcaga aagttaatca
     3061 acgcgctccg gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc
     3121 agccatcggg aatcgacggc agctcgatac gcccggcttt cgccagaggc gctaacgcat
     3181 cgtagtagtt ttgccacagc gccagacgtt gctggttgat acgatccgct gcttccagtt
     3241 gcgcccacag gtacgcagct tgcagatcgg acatcaaata gctggagcca atatcgcgcc
     3301 aggtatattt atcgacctga ccacggaaga actggctgcg gttagtgccc ttttcacgga
     3361 tgatctcggc tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg ccttcaccac
     3421 ccgccgtata gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca
     3481 gtgcacgccc tttgtaagtg gacatcacgc cctgagcggc atcttctacc acaaacagat
     3541 tatgcttttt cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga
     3601 ccggcacgat aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt tcgtcgatgt
     3661 tcatggtgtc cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc acaaaggcat
     3721 tggcggtgga gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga
     3781 gcagcagcgc cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc
     3841 tgccaaaacg ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca
     3901 gtttgccgct acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg
     3961 cgttaaatgg aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca
     4021 ccactttgta tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa
     4081 gttgttttac cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctaca
     4141 ccgcgtccag ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca
     4201 taaccacgaa taccgccgga cgccgcacgt aaaatcagac attgatgatc aaaggtgccg
     4261 cgcacggcat tttcaatcca ctgtgcataa aagcgtctgc tggcgtcagg cgcataccac
     4321 ggcgcacgaa aacggctttg cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata
     4381 tcggtctctt gtgccactac agcaccacta tcactgacat tgttcacggg tagtgccaaa
     4441 tcaacttcac cttctaccag ggagaatccc agctgttgca gggcatccag ttcacccgta
     4501 tttgatgccg caattttggc ctgcacccgt gaccacggcg ctaacacgtc tggcgtcagg
     4561 agcggtgctt cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc
     4621 caggttagtg gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc
     4681 gtagccagtt ttcgctaatg aactggcagc acgcttcaca ccctcgtcat cgagccagcc
     4741 gttacgccag gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttctactgt
     4801 ctgcacaaag gtactggctt caatcaggct gtcgtgagtg ccagtgtcca gccaggcaaa
     4861 tccgcgcccg agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga
     4921 ggtaatctcc agttcaccac gctccgacgg cttcacctgc tttgcgtact ccacgacttt
     4981 actgtcgtag aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc
     5041 ttccagcgag atagcgcgga aattgtcgtc aaactccacc acgccaaagc gttccgggtc
     5101 catcacctga tagccaaata ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt
     5161 cggactgaag ccctgaccga agaagatgtt atcgcccagc accagacaag aaggttcacc
     5221 gttgaggaag gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc
     5281 ggcgtattcc agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc
     5341 tttatcttcc ggcgtagtga tgatgagaat ttcgcggata ccggccaaca tcagcaccga
     5401 cagcggatag taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg
     5461 cgtaatcgga tgcaatcggg tgccggaacc gcccgccagg ataatacctt tcatgtttgc
     5521 ctccgctgaa aattagcctt tcagacctaa acgctcgccc tgatagctgc cgtcctgcac
     5581 ctgcttccac cagctttcat tagccagata ccactgcacc gttttacgca ttccactttc
     5641 aaaggtttcc tgtggcagcc aaccaagttc acgggcaatt ttcgaagcat caatggcata
     5701 acgcagatca tgccccggac ggtcagcgac aaaggtgatc aagtcacgat agtgcgccac
     5761 gccgtgcggc ttgttcggag ccagttcttc cagcagctcg caaatggttt ccacaacatc
     5821 gagattctta cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt
     5881 cgccacgcaa tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg
     5941 cccgttgcca tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat
     6001 cagtttttcc ggaaagtggt aaggaccgta gttattcgag cagttggtga tcagcgtcgg
     6061 cagaccgtag gtccgcagcc aggcgcgcac cagatggtcg ctgctggctt ttgacgcgga
     6121 ataagggctg ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag
     6181 gtcaccatat acttcgtcgg tggagatatg atgaaaacgg aacgctgatt ttttatcttc
     6241 cgtcagcgta ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt
     6301 ggtttcaata aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag
     6361 atgcatgaca cagtctggct gatgctcagt gaatacgcgt gccagttctg cccgatcgca
     6421 gatatcaact ttctcaaagg caaagcgctc gctttgcgcg accggtgcca gcgacatcag
     6481 gtttccggcg taggtcagct tatcgaccac taccaccgcg tcgctcgttt cgttgatgat
     6541 ataacgcacc agcgccgagc caataaaccc ggcaccacct gttatcagaa tttttctcat
     6601 cagcgccaga ctcctttggc atcgacgaca tactgctgat ggacattgtc gccattgata
     6661 actttgaact gactatgatc gaccagcatc accagcacgt ctgccgttgc cagcgcctcg
     6721 tcaagctgcg ccagagtaca aagcccggtc agttttttcg gcaactggtg gatgttaggc
     6781 tcaacaacca gagtttcgcc gctatgccac tgggcgatca gttcagcgat ttccatcgcc
     6841 gggctttcgc gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc
     6901 agttcactgg cgcgtttatc ggtagcagcc aggcaatcag ccaccgccgc tttcacctga
     6961 tcgataaccc agaacggttt gtgatcgttc acttcgcgtg cggtacggat aagccgcgcc
     7021 tgctgggggt tctgtgccac gataaaccac ggatcaacag caatgcagtg accgcccacg
     7081 ccagggccag gctgaagaat attaacgcga gggtgacgat tcgccaggcg aatcagttcc
     7141 cagacgttaa tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc
     7201 acatcgcgga agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg
     7261 acacactcac cttcgaggaa aattttgtac agttcgctgg cgcgggccga acaaaccggc
     7321 gtcataccac caatcacgcg atcgttttta atcagctcga ccataacctg gcccggtaac
     7381 acgcgttccg ggcagtaagc aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa
     7441 gtgagatccg gacgcatctc tgctaaccat tctgccatct tctcggttga ccccaccggc
     7501 gaggtggatt caaggatcac cagcgcgcct tttttcagca ctggcgcaat ggagcgagca
     7561 gccgattcaa cgtaggtcat atctggctca tgatcgcctt taaacggcgt aggtacagca
     7621 atcagccagg catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg
     7681 gcagttttta ctacgctcgc caaatcaggt tcgacgatat ggatttcgcc acgattgatg
     7741 gtatcaaccg cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac
     7801 gcagctgctg taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc
     7861 atagtgatat ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac
     7921 catacgggtt atgggcgcgg ctcatagctt gatattcgtt ttcgtctttt aaaagacgcg
     7981 tcacttcctc gacaattcgc tgcttatccg tgcctaccag acgcaccgta cccgccgtca
     8041 ccgcttccgg acgctcagtg gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg
     8101 cttcttcctg aatgccgcct gagtcggtca aaatcagcca ggcgtggttc atcagccaga
     8161 caaacggtaa atactcctgg ggatcgatca gaatgacatt tttcacatgc cccagaatgc
     8221 gattgaccgg ctctctgacg ttcgggttga gatgcaccgg atagacaatc tggatgtcct
     8281 ggtgcgtggt ggcgatgtct gccagcgcgt ggcagatttc ttcaaagcca cgaccgaaac
     8341 tttcacgcct gtgaccggtc accagaatca tctttttatc ggggtcgata aacgggtaat
     8401 ttgccgccag ttctgaacgc agcgtgtcgc tgctcatcac ctgatcacgc acccataaca
     8461 gtgcatcaat gactgtatta ccagtaatga agattcggct atccgcaacg ttttcacgca
     8521 gcaagttttg ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca
     8581 atgtacggtt agcctcttcc ggccacggcg aatagagatc gcccgtgcgt aaaccagcct
     8641 caacgtgacc aacaggaata cgctgataaa acgccgccag gctggttgcc agcgtcgttg
     8701 ttgtgtcgcc gtgaaccagc acgacgtctg gtttgaactc ggcaagaata ggttttagcc
     8761 cttccagaat ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat
     8821 cgtagtcagg tacaatggaa aagagtttca gcacctgatc gagcatctcc cgatgctgcg
     8881 cagtgacgca aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggag
     8941 ccatcttgat ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct
     9001 tcgaataagc ggcgagcgcc tttgcgctcg cagcagcagt gttgctattt cgagcaacgg
     9061 cgggttaatg cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg
     9121 aaggcacgac gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag
     9181 gtctgaaaac gcggatccag ggttggacca acattcaggg tgtttaacat ggcccgattc
     9241 tgatcatagt cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg
     9301 agcattggac gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct
     9361 gtcgcactgc gcgaaatatt atgctgctca gcaattttca gcgcctgctc aatgctgttc
     9421 atccggcggt cgtagatggc tttcgccacc tcttcctgac gcttcacctg agctttcatc
     9481 tggatggtgc gtgccgccca tgcgcctttc agctcatcat tcagatggct ggctgcacgc
     9541 tggctggcaa aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcggca
     9601 ataagcttca cgctgtcatt gaccgcgcgg gtaaagtctc cggggataaa ctggatgttg
     9661 ttaatcattt catccagcaa cgccgcatcg gctttgctgt tgcccaccat ccgctgcttg
     9721 taatagtcgg tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca
     9781 aactctttat aggcttcgtc catgaccgat ggttggtcgg cagaagccat gtttgaacgg
     9841 gcatccaggt tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat attcaccgtt
     9901 ggacgatcgg taatcgccgt cgcgctccac tcttgtcgag caaaaaaagt atacgccagc
     9961 gcgattaacg caaacgccag ccccatgcca ataatccata gcttcccagc ccacaaggta
    10021 cgaaacaacc cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt
    10081 ggttgtgtca tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac
    10141 gcagtctgcg ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat
    10201 atccatagag gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat
    10261 gagaatattc tgccagcacg ccaatggaag cgagcagtgc tgcggcaagg gtaatcagca
    10321 caaacgcctg acgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt
    10381 caggagagaa tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca
    10441 ttagcggaat ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct
    10501 gggtcgtttc gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac
    10561 ccataaagac tttgtagcgg cgacccagga taccaaggtt aagcatgatg tatggcagga
    10621 tggcggcgat catcgcaaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa
    10681 tcataccgat tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat
    10741 caaccatgtt gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtaaccaa
    10801 acggtccgag caccatctcc caggagccaa agatataacc caggctactg agataaagtt
    10861 tgccgaacac catcataaca atgccaacag cggcctgtat ggtggcacgg attttcacgc
    10921 tgatatcaaa acggtcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat
    10981 agagagatgc atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt
    11041 aaaccgaaat ccccccaacg agaggtatca atccctggtg acgtttgcgg aagtttggtt
    11101 tatccactaa accgactttt tttgccacct tacgggcaaa aaacagaaac agtgtcgtga
    11161 ataaaaaaat actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca
    11221 gagaaaatta ttagcagaag tataaccacg aagaccttta ttcagaaggg aaagtccgtt
    11281 ccgaacccac cagccctgtg caaaaattaa ccatttgata tgcaaattgc ataacatgcc
    11341 agcaaattcc aggaattttc ctaacatcgc cagcgcggca ttaagattac agatataaaa
    11401 gcaaaacgcc acgtaaacac gtggcgtttt tggcataaga caaatttatg agcgtttcat
    11461 catttcgaag aaatcgtcat tggtcttggt catggccagt ttattaatga ggaattccat
    11521 tgcatcgatt tcgcccatcg ggtgaatgat tttgcgcagg atccacattt tctgcagttc
    11581 ttcctgagtc gtgagcagct cttctttacg ggtaccggaa cggttgtagt cgatagccgg
    11641 gaagacgcgt ttttcagcga tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt
    11701 aaactcttcg taaataactt cgtccatttt agaaccggta tcgataagcg ccgtcgcgat
    11761 aatggtcagg ctgccgccct cttccacgtt acgcgccgca ccgaagaagc gtttcggacg
    11821 atgaagggca ttggcgtcca caccaccggt caacacttta cctgacgccg gaacaacagt
    11881 gttgtaagcg cgcgccagac gagtgatgga gtcgagcaga atgataacgt ctttcttgtg
    11941 ctcaaccagg cgtttggcct tctcgatcac catttccgca acctgaacgt ggcgagatgc
    12001 gggttcgtca aaggtagaag caacaacttc accttttacc agacgctgca tctcggttac
    12061 ttcttccgga cgttcgtcga tcagcagaac catcagcaca caatccgggt ggttgtaagc
    12121 aatgctctga gcaatgttct gcagcagcat agttttaccg gctttcggcg gtgccacaat
    12181 cagaccacgc tgaccacgac cgataggtga tgccagatcc agtacgcgag cggttaaatc
    12241 ttcagtagaa ccgttaccac gttccatacg cagacgagag tttgcgtgca gcggggttaa
    12301 gttctcaaag aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac
    12361 tttcagcagc gcaaaatagc gttcaccttc tttcggcggg cgaatcttac cagagatggt
    12421 atcaccagtg cggaggttga aacggcggat ttggctaggg gaaacgtaga tgtcatcagg
    12481 accggcgagg taggagctgt ctgcggaacg gaggaaacca aatccatcct gcaatatctc
    12541 cagtacgcca tcaccaaaga tatcttcgcc actctttgcg tgctgcttca ggatggcaaa
    12601 aataatgtcc tgcttacgca tacgagccag gttttccagc cccatatttt cgccgagagt
    12661 gatcagctca gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg tgtgagttct
    12721 taaacttggg gtaaatctcg aacttaatgt tgtgaatggt atggcaggat catccatgcc
    12781 tgtttacggt catcgactca tgtcttttcg ctgcctggtc acaggagagt acgcagaact
    12841 gaaacgacaa gacggattga gtgacgagcc agaaatctgt tcacttcgca tttaagataa
    12901 aacgggaagc gttggaaatt acaagattca aacttaataa ggtatgttta atacgaagtc
    12961 aacactaagt tagcatgact cacgccgggc gtccagtttt tagcgacggg gcacccgaac
    13021 atgaaattcc cttacgccag gttagcgtcg aggaactctt tcaactgacc tttagacagt
    13081 gcacccactt tggttgccgc cacttcaccg tttttgaaca gcagcagagt cgggatacca
    13141 cggatgccat atttcggcgc agtgccaggg ttttgatcga tgttcagttt tgcaacggtc
    13201 agtttgccct gatattcgtc agcgatttca tccagaatcg gggcgatcat tttgcacgga
    13261 ccgcaccact ctgcccagaa atcgacgagg atcgccccgt ccgctttgag tacatccgtg
    13321 tcaaaactgt cgtcagtcag gtgaataatt ttatcgctca tatataactc cacaggaata
    13381 agcctggcgt gttggtttcg ttgttggtgt aacattaacc aactaaaggt tgactttatt
    13441 tcaccggata cgctttcgta aagcaatagt aagctgatat tctaccacac tatgagcaaa
    13501 acacatttaa cagaacagaa gttttccgac ttcgccctgc atccgaaggt tgtagaagcc
    13561 cttgaaaaaa aagggtttca taactgtacg cccattcagg cactggccct tccgctgacg
    13621 ctggcgggtc gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac gatggcgttt
    13681 cttacgtcaa cgtttcatta tcttctctct catcctgcga ttgccgatcg caaggtgaat
    13741 caaccacgtg ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat ccatgccgac
    13801 gcagaaccgc tggcggaagc taccggcctg aagctgggtc tggcttacgg tggtgatggc
    13861 tacgacaaac agctgaaagt gctggaaagc ggcgttgaca ttctgattgg cactacgggg
    13921 cgtttaattg actacgccaa gcagaaccac attaacctcg gtgccattca ggtggtggta
    13981 ctggacgaag ccgatcgcat gtacgatctg ggctttatta aagatatccg ctggctgttc
    14041 cgccgtatgc cgcctgcaaa ccagcgcctc aacatgctgt tctccgccac gctttcgtac
    14101 cgggtacgtg aactggcgtt cgagcagatg aacaatgccg aatatattga agtggaaccg
    14161 gaacagaaaa cgggccaccg tattaaagaa gagcttttct acccttctaa cgaagaaaaa
    14221 atgcgtttgc tgcaaacgct gatcgaagaa gagtggccag acagagcgat tattttcgcc
    14281 aataccaaac accgttgtga agagatctgg ggccacctgg cggcagatgg tcatcgtgtc
    14341 ggtttgttga caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc
    14401 cgtggcgatc tggatattct ggttgccacc gacgttgccg cgcgtggttt gcatattccg
    14461 gcagtgacgc acgtctttaa ctacgattta cccgatgact gtgaagatta cgttcaccgt
    14521 attggtcgta caggtcgcgc aggcgcaagc ggtcactcta tcagcctggc gtgtgaagag
    14581 tatgcattga atttgcctgc tattgagacc tatattggtc actcaattcc ggtaagcaaa
    14641 tacaatccgg acgcattgat gaccgatctg ccaaaaccgc tgcgcctcac gcgcccacgt
    14701 acaggcaatg gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata
    14761 aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg caagagaatg aatcccgatg
    14821 agcatactgt agtaagtgat tcaggtgaaa gaatgcagcc aacacagaga cagattgaag
    14881 gatgaagagt atgggttcca cctcgtcgct gtatgcagcc attgatctcg gttcgaatag
    14941 ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc cagacgctga cgcgaataaa
    15001 acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat gccctgtcta atgaagcaat
    15061 ggagcgcggt tggcaatgtc tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc
    15121 gcaaattcgc gttgtcgcta cggcgacatt acgtcttgcc gtcaatgcgg gtgattttat
    15181 tgccaaagcg caggaaatcc tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc
    15241 acgtctgatt tatcagggcg ttgctcacac aactggcggt gccgatcagc gcctggtggt
    15301 ggatataggc ggtgccagta ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt
    15361 gttcagcctg tcgatgggct gcgtcacctg gctggaacgc tattttgccg atcgtaatct
    15421 ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc
    15481 cgatgaatta cggtatcacg gctggaaagt gtgcgttggt gcttccggca ccgtgcaggc
    15541 gttacaggaa atcatgatgg cacaggggat ggatgaacgc attaccctgg aaaagttgca
    15601 gcaattgaaa cagcgagcca ttcattgcgg tcggctggag gaactggaga ttgacgggct
    15661 gacgctggaa cgtgcgttag tgttcccgag tggtctggcg atcctgatcg ccatttttac
    15721 cgaactgaat attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta
    15781 cggcatgttg catctggccg tcgagcagga tattcgcagc cgtacgctgc gtaatattca
    15841 gcgccgcttt atgatcgata ttgatcaggc gcagcgcgta gccaaagttg cggctaactt
    15901 cttcgatcag gtggaaaagg aatggcatct tgaagcaata agccgcgatt tgctcatcag
    15961 cgcctgtcag cttcatgaaa tcggcctgag cgttgacttt aaacaagcgc cgcaacacgc
    16021 tgcttatctg gtgcgtaatc tggatcttcc cggttttacc cccgcacaga aaaaattgct
    16081 ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc tcatcgctgc atcagcaaaa
    16141 tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta ctccgcctgg ccatcatttt
    16201 tgccagccgt cgccgtgacg atctcgtgcc agagatgaca ttacaggcta accatgaact
    16261 gttgaccttg acgcttccgc aaggttggct aacccaacat ccgctgggta aagagattat
    16321 tgctcaggaa agccagtggc agagctatgt ccactggccg ctggaagtgc attaatgagt
    16381 aagtgccgga tgcgatgctg acgcatcttt tccggccttg attatttccc tcgttttgcc
    16441 gccatcatcg ctttcagatt cgccagatgg ctttgccctt tctgcatccg ttcttcggcg
    16501 ctgaccactt tgcgctcctg ttcccaaatc agatcatcct gcggcagctc cagcaaaaag
    16561 cggctcggct ccgggcgcac cagctcgccg tactgacggc gttctttaca cagcgtaaag
    16621 gtcaattcct tctgggcgcg ggtaatgccg acataggcca gccgccgctc ctcatcgata
    16681 ttatcttcat cgatgctgct ctggtgcggc aaaaaccctt cttccatacc gaccatgtag
    16741 acataaggaa actccagccc ttttgacgcg tgcagagtca tcagttgcac ctgatccagc
    16801 tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag taaagcgcgt caccacctgg
    16861 gtgagcgtca tcggctcatc cagttcgctg ccttccagca tctccgtcat ccagctaaac
    16921 agttggttga cgttcttcat gcgcatttcg gcggctttcg ggctgggcga tgtttcgtac
    16981 agccaggatt cataatccat gccgtggatc agatcgcgca ccgcggcaat cggctcccgc
    17041 tccgccagac gctggatttc tgccaaccag tgagtaaagc gggtcaatgc ttcataacca
    17101 cgtccgctaa gcgtctgact caggcccata tcaaagctgg cggtaaacat gcttttattg
    17161 cgcgtcatcg cccactcacc cagctttttc agcgtcgccg ggccaatctc tctcttcggc
    17221 gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa
    17281 gccagcaagt ccttgatttc aggacgagag aaaaacgacg taccaccaga aattttgtac
    17341 gggatgcggt tttgcatcag gaatttttca aacactcgcg actgatggtt accgcgataa
    17401 agaatagcgt aatctttgta ctgcgtttta ttgacgaagt gatgcgcaat cagttcacca
    17461 gtgacgcgct cggcttcatg ttcttcgtta ttcgcgctta aaactttaag ctctgtgccg
    17521 taacccagtt cggagaacag acgcttctca aagacgtgcg ggttattggc gatcaggatg
    17581 ttcgccgctt tcaaaatacg tccggaagag cggtagttct gctcaagctt aatcaccttc
    17641 agcgccggaa aatcctgact cagcagcacc aggttttgtg gacgtgcgcc gcgccaggag
    17701 tagatcgact ggtcgtcatc gcccaccacg gtaaagcgcg cgcggctgcc caccagcagt
    17761 ttcaccagct catactggct ggtgtttgtg tcctgatact catccaccag cagatagcga
    17821 attttgttct gccagcgctc gcggacttct tcattgcgtt gcagcagcaa cgtcggcaat
    17881 aaaatcagat catcgaagtc gagaacattg cacgctttca ggtgtgcatc atacagccca
    17941 taacaatggg caaaaatacg atcccgctcg ccctttgcct ctgccgccgc ctgcgccgga
    18001 gttttgagat cgttcttcca gttggagatg gtcgaaatca gctgttgcag gagaactttg
    18061 tcatcttcaa tcagcccctc ggtcaactct ttaagcaaag caagctgatc ggtatcgtca
    18121 aacagcgaaa agttcgcctt catcccaagc gccgcatact cgcgtttgat gatatccaac
    18181 cccagcgtgt gaaaagtgga gatcatcagc ccgcgcgcct ctttgcgacc cagcgtctgc
    18241 ccgacacgct ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt
    18301 cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt tattggtgat cacacgagtt
    18361 ttaccggaac ccgcgcccgc cagcaccagg caggggccgg taacgaattc gacagcttgt
    18421 tgttggccgg ggtttagacg cataggtgta ttgctcaatc ttcgaacggg gggaggattg
    18481 tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct aaccggttgg ctgcggatgt
    18541 ttaaatcaga gagagaagac cgaaatcgga gttcatcgtg aatccattag acttagaaaa
    18601 tatcgggtcg taacgttgtg ttattacttt ttcagagttt actaaggaga ggtttactcg
    18661 tggcgattaa tttcagccct aaggttggtg aaatacagga atgcaacttt ggtaactatc
    18721 ctgttatcca gaatggacag ttcagcacca cgtattacga tggccgcatt cctcctgaaa
    18781 tgataaaaaa caggttagtt gtcgtgctca acggaaaaat taatggcaac gcatgtatcg
    18841 tcgttccatt atcaaccact cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa
    18901 tagccagtaa tgttatcaat gtcctgcaat tctttgatca gcagattcgt tgggccaaag
    18961 ccgacctggt acaacaagtc agtcgaaacc gattaaatag agcaaggact tacagagggt
    19021 atcttaatca atgtttgcca catgagttgg ttgcagacat acagagggcg gtcatcaagt
    19081 caatcaatgc catatcgctc ataaattgac aataaaataa ccatccccga taatgacttt
    19141 gtagcccata gaggccctaa gttttctccc cttgagggac ctgcgaagcc cggattttcc
    19201 gggcttttgc catatatgta ccagcccctc cctgactgac aattacgtta gcatctgcgg
    19261 atcatgctaa aatcgccgcc tgacaattat tcactcaagg cacgatcatg gcaaaaacag
    19321 cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggatctt ctcgagcaga
    19381 ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca
    19441 aacgcggcgg tgatttaggt gaattccgcc agggtcagat ggttccggcg ttcgataaag
    19501 ttgtgttctc ttgtccggta ctggagccga ccggcccgct gcacacccag ttcggttacc
    19561 acatcattaa ggtgctgtac cgcaactaat agcaaggcct tctccaggag aaggccttga
    19621 gtgttttctc cctctccctg tgggagaggg tcggggtgag ggcatcagcg cgcacttaac
    19681 ccgcaacagc aatacgtttc atatctgtca tatagccgcg cagtttctta cctacctgct
    19741 caatcgcatg gctgcgaatc gcttcgttca catcacgcag ttgcgcgtta tctaccgcgc
    19801 cttccggaat agctttaccc aagtcgcccg gttgcagctc tgccataaac ggtttcagca
    19861 acggcacaca agcgtaagag aacagatagt taccgtactc agcggtatca gagataacca
    19921 cgttcatttc gtacagacgc ttacgggcga tggtgttggc aatcagcggc agctcgtgca
    19981 gtgattcata atatgcagac tcttcaatga tgccggaatc gaccatggtt tcgaacgcca
    20041 gttcaacgcc cgctttcacc atcgcgatca tcagtacgcc tttatcgaag tactcctgct
    20101 cgccgatttt gccttcatac tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac
    20161 gccaggtcag cagtttctta tcatcgttgg cccagtccgc catcataccg gaggagaatt
    20221 cgccggagat gatgtcgtcc atatgtttct ggaacagggg tgccatgatc tctttcagct
    20281 gttcagacag cgcgtaagca cgcagtttcg ccgggttaga gagacggtcc atcatcaggg
    20341 tgatgccgcc ctgcttcagc gcttcggtga tggtttccca accgaactga attagttttt
    20401 ctgcgtatgc cggatcggtg ccctcttcca ccagcttgtc gaagcacagc agcgagccag
    20461 cctgcaacat accgcacagg atggtttgct cacccatcag gtcagatttc acttccgcaa
    20521 cgaaggacga ttccagcacg cccgcacggt gaccaccggt tgctgccgcc catgctttag
    20581 cgatcgccat accttcgcct ttcggatcgt tttccgggtg aacggcaatc agcgtcggta
    20641 cgccgaatcc acgtttgtac tcttcacgca cttcggtgcc agggcatttc ggcgcaacca
    20701 tgactacggt gatatcttta cggatctgct cgcccacttc gacgatgttg aaaccgtgcg
    20761 agtaacccag cgccgcgccg tctttcatca gtggctgtac ggtgcgcact acatcagagt
    20821 gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg cgggatcagt tcttcgtaag
    20881 taccaacttt aaaaccgttt tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg
    20941 cttctttacg cagagcgtag gagatatcga gaccagaatc acgcatgttc aggccctggt
    21001 tcagcccctg tgcgccacag ccgacgataa ccactttttt accctgaagg tagctcgcgc
    21061 cgtcggcgaa ttcgtcgcgc cccataaagc gacatttgcc cagctgtgcc agctgctggc
    21121 gcaggttcag tgtattgaag tagttagcca tggtgattcc tcgtgatgtt gtgcttctta
    21181 ttgttcggtt cgcgtttgcg agattgaatt cactatatga caggaaattt attgcggaaa
    21241 ttgatatatt cacaacgtca cattgcaatt tctgcaacgt caacattgag ggctgcgcca
    21301 ttggatttac gcgatctgaa aaccttcctg catctggcgg aaagccgcca ttttggccgc
    21361 agcgcgcggg cgatgcacgt tagcccatcc acgctctcac ggcagattca gcgcctggaa
    21421 gaagatcttg gtcagccgct atttgtgcgc gataaccgca cggtaacgct gaccgaagcg
    21481 ggcgaagagt tacgcgtttt cgcccagcaa acattgttgc agtatcagca actgcgccac
    21541 accatcgatc agcaagggcc gtcgctctct ggcgagttac atattttctg ctcggtgacc
    21601 gccgcttaca gccatctacc gccgattctc gaccgcttcc gcgcggaaca cccgtcggtg
    21661 gagatcaaac tcaccactgg tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa
    21721 gcggatctgg cgattgccgg gaaaccggag acgctacccg gcgctgtggc gttttcgatg
    21781 ctggagaatc tggcagtagt gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag
    21841 gtatctgtcg aaaagcccga ctggtcaacg gtgccgttta ttatggccga tcaggggccg
    21901 gtacgccgcc gcattgaact gtggtttcga cgcaataaaa tcagcaaccc gatgatttac
    21961 gccacggttg gcgggcatga agcgatggta tcgatggtgg cgctcggctg tggtgtggcg
    22021 ctgttgccgg aagtggtgct ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt
    22081 ttagatcgca gcgatgagaa aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg
    22141 ctgcatgagc cgctgattga ggcgttctgg aggattttgc cgaaccacaa atgacgttgt
    22201 cgcgcg
//
LOCUS       DAFATA010000075        22143 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFATA010000075 DAFATA010000000
VERSION     DAFATA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.076"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="LGJ20_003311"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="LGJ20_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBK2387332.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="LGJ20_003312"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="LGJ20_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBK2387333.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="LGJ20_003313"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="LGJ20_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBK2387334.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="LGJ20_003314"
     CDS             complement(1087..1581)
                     /locus_tag="LGJ20_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBK2387335.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="LGJ20_003315"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="LGJ20_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBK2387336.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNLNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="LGJ20_003316"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="LGJ20_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBK2387337.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="LGJ20_003317"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="LGJ20_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBK2387338.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="LGJ20_003318"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="LGJ20_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBK2387339.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /locus_tag="LGJ20_003319"
     CDS             complement(6745..7032)
                     /locus_tag="LGJ20_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBK2387340.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /locus_tag="LGJ20_003320"
     CDS             complement(7151..8038)
                     /locus_tag="LGJ20_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBK2387341.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="LGJ20_003321"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="LGJ20_003321"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBK2387342.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="LGJ20_003322"
     CDS             complement(9275..9769)
                     /locus_tag="LGJ20_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBK2387343.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="LGJ20_003323"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="LGJ20_003323"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBK2387344.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="LGJ20_003324"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="LGJ20_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBK2387345.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="LGJ20_003325"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="LGJ20_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBK2387346.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="LGJ20_003326"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="LGJ20_003326"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBK2387347.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDSFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /locus_tag="LGJ20_003327"
     CDS             13861..14520
                     /locus_tag="LGJ20_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2387348.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="LGJ20_003328"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="LGJ20_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBK2387349.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="LGJ20_003329"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="LGJ20_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK2387350.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWGATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="LGJ20_003330"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="LGJ20_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBK2387351.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="LGJ20_003331"
     CDS             17948..18874
                     /locus_tag="LGJ20_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBK2387352.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="LGJ20_003332"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="LGJ20_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBK2387353.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="LGJ20_003333"
     CDS             complement(19225..21444)
                     /locus_tag="LGJ20_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBK2387354.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..>22143)
                     /locus_tag="LGJ20_003334"
     CDS             complement(21555..>22143)
                     /locus_tag="LGJ20_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HBK2387355.1"
                     /translation="GERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTL
                     VLTLRPTGLLPLVTDSLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVT
                     AQQGTWERWTVRADEPQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVY
                     FGQPSWAHFPFYFNSQTLEMADRGSIGQLLVNPVP"
BASE COUNT         5483 a         5933 c         5584 g         5143 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaggttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg ccccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgcc
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttaacaa
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagc gaccccattg
     3901 aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt
     3961 ttagtcgctg tagcgacacc ttccacccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgtta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat agcttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc ggattgtata acattgccac ttttggacaa
    13741 ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgattca ggagtacaat aggcaaataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccagg gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgtttcc agtttttcaa ctggttaagc
    14341 ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc tgttcccggt tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 actggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg ggggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct gctttttcgg
    20641 tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg ggattgggtc
    20701 gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag gcagcgcctc
    20761 tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    20821 cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgtgcggt
    20941 acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg
    21061 cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga tcatcgaatc
    21121 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca tgaaatcgtc
    21181 tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg
    21901 atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 ccc
//
LOCUS       DAFATA010000076        21859 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFATA010000076 DAFATA010000000
VERSION     DAFATA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.077"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..222)
                     /locus_tag="LGJ20_003335"
     CDS             complement(<1..222)
                     /locus_tag="LGJ20_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2387356.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLK"
     gene            complement(222..404)
                     /locus_tag="LGJ20_003336"
     CDS             complement(222..404)
                     /locus_tag="LGJ20_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387357.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(416..1912)
                     /locus_tag="LGJ20_003337"
     CDS             complement(416..1912)
                     /locus_tag="LGJ20_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK2387358.1"
                     /translation="MPITLEPWQLFVICCAFGWVNKGTRLRRFREVYTEIPRKNGKSA
                     ISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPARLMCKRTPMLTEAFGIEVNAS
                     NMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHATDALYTTMLTGMGARRQPLMWA
                     ITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIYTVDEGDDWTDPQVLEKANPNI
                     GVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKSLTLE
                     QFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNED
                     RRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHD
                     LADEDLNPITIIQNYTNMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKTIPGND
                     DVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLSDHIESYGIRSL"
     gene            complement(2146..2640)
                     /locus_tag="LGJ20_003338"
     CDS             complement(2146..2640)
                     /locus_tag="LGJ20_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBK2387359.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGKKKATNPFLK
                     IIES"
     gene            complement(2766..3116)
                     /locus_tag="LGJ20_003339"
     CDS             complement(2766..3116)
                     /locus_tag="LGJ20_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK2387360.1"
                     /translation="MPPRTPKACRVRGCRNTTTDPSGYCESHKSEGWKQYKPGLSRHQ
                     RGYGSKWDTIRERVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            complement(3677..4069)
                     /locus_tag="LGJ20_003340"
     CDS             complement(3677..4069)
                     /locus_tag="LGJ20_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HBK2387361.1"
                     /translation="MKLRYKLVIVAFVVSVIGSFIWSAGHYYSKYQHEKERADEAVRN
                     AESATAITRNVLQSLQIINTVIEANQHAKQQIALESQRTQEDIKVAVADDDCASRHVP
                     AAAADRLRKYANSLRAGSGGTAANKPDH"
     gene            complement(4053..4529)
                     /locus_tag="LGJ20_003341"
     CDS             complement(4053..4529)
                     /locus_tag="LGJ20_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBK2387362.1"
                     /translation="MQALNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGSIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKKAGGVVNEAEI"
     gene            complement(4533..4868)
                     /locus_tag="LGJ20_003342"
     CDS             complement(4533..4868)
                     /locus_tag="LGJ20_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HBK2387363.1"
                     /translation="MPHNPNTWPDWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGG
                     GGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVI
                     GNKFGSSKE"
     gene            complement(4945..5997)
                     /locus_tag="LGJ20_003343"
     CDS             complement(4945..5997)
                     /locus_tag="LGJ20_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBK2387364.1"
                     /translation="MKNTVKINSVDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWTHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTVKEVSELVGK"
     gene            complement(6148..6351)
                     /locus_tag="LGJ20_003344"
     CDS             complement(6148..6351)
                     /locus_tag="LGJ20_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387365.1"
                     /translation="MLKQQDMTETAAAVLHFLPADKWVTPRMMTRTTGVSEARCQLIL
                     TQLVLAGLAKDNGGYGNKFRRCQ"
     gene            complement(6604..7356)
                     /locus_tag="LGJ20_003345"
     CDS             complement(6604..7356)
                     /locus_tag="LGJ20_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBK2387366.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTK"
     gene            complement(7370..8359)
                     /locus_tag="LGJ20_003346"
     CDS             complement(7370..8359)
                     /locus_tag="LGJ20_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBK2387367.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FSSGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(8367..9176)
                     /locus_tag="LGJ20_003347"
     CDS             complement(8367..9176)
                     /locus_tag="LGJ20_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBK2387368.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKNRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEQNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSVPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(9196..9585)
                     /locus_tag="LGJ20_003348"
     CDS             complement(9196..9585)
                     /locus_tag="LGJ20_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBK2387369.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPGGKVEITISKYEKTAGAAA"
     gene            complement(9588..9908)
                     /locus_tag="LGJ20_003349"
     CDS             complement(9588..9908)
                     /locus_tag="LGJ20_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBK2387370.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRTLLAGEENARLRAAHWLH
                     ERGL"
     gene            complement(9905..10558)
                     /locus_tag="LGJ20_003350"
     CDS             complement(9905..10558)
                     /locus_tag="LGJ20_003350"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HBK2387371.1"
                     /translation="MSNKYCQALVELRNKPAHELKDVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLVQVRQQAEKLVREMAA"
     gene            complement(10558..11052)
                     /locus_tag="LGJ20_003351"
     CDS             complement(10558..11052)
                     /locus_tag="LGJ20_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBK2387372.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYIGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(11049..11867)
                     /locus_tag="LGJ20_003352"
     CDS             complement(11049..11867)
                     /locus_tag="LGJ20_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2387373.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSPGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKSSYDNVDYSQIPAGFRG"
     gene            complement(11864..12100)
                     /locus_tag="LGJ20_003353"
     CDS             complement(11864..12100)
                     /locus_tag="LGJ20_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387374.1"
                     /translation="MLNPLILNICRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            complement(12093..12929)
                     /locus_tag="LGJ20_003354"
     CDS             complement(12093..12929)
                     /locus_tag="LGJ20_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBK2387375.1"
                     /translation="MNSLTANNRLSQQLVVSVAEHLLLRHECRLPNHLAVSNHRELYL
                     TVGGELCRNLTAGFVTEEDFMFMLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLS
                     VKDHQHIDSAIPGRYTVQAPYKAGAGIGVLELIKAIYDAPASFLSSAFAHIKVMVGWA
                     GASKDAPVSLITGYANPVQFTTSEIGVSGGGSISPIKEAAIMATVPALTRLNDEDLHK
                     LSYVTTALRALRKVTLSDPQAHQVLVETLLNLQAERIRLADKANFHIHRLLNISGGHR
                     HA"
     gene            complement(12926..13477)
                     /locus_tag="LGJ20_003355"
     CDS             complement(12926..13477)
                     /locus_tag="LGJ20_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387376.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(13521..13721)
                     /locus_tag="LGJ20_003356"
     CDS             complement(13521..13721)
                     /locus_tag="LGJ20_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2387377.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            13812..14486
                     /locus_tag="LGJ20_003357"
     CDS             13812..14486
                     /locus_tag="LGJ20_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBK2387378.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            15155..15517
                     /locus_tag="LGJ20_003358"
     CDS             15155..15517
                     /locus_tag="LGJ20_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387379.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            15583..16407
                     /locus_tag="LGJ20_003359"
     CDS             15583..16407
                     /locus_tag="LGJ20_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HBK2387380.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            16536..17060
                     /gene="yfdR"
                     /locus_tag="LGJ20_003360"
     CDS             16536..17060
                     /gene="yfdR"
                     /locus_tag="LGJ20_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBK2387381.1"
                     /translation="MSFIKTFSGKHFYYDRINKDNIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHT
                     YGMFMERFKELTES"
     gene            17169..18035
                     /locus_tag="LGJ20_003361"
     CDS             17169..18035
                     /locus_tag="LGJ20_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387382.1"
                     /translation="MNQNPFSFYDFLGYLIPGGFFILLMYFCGLTFDLDIVIDLSELL
                     RGQSQIFGILNYASIVIISYIAGHFISITSAFFIEKYMNKELGYPSKYLFKKLIDTSE
                     SICSPSCDERSADKKTKIKNRIIKCVLCPIILWDFITQKLCYSQSLPFHLANTTWLMI
                     KEGYEKKFITNRQLLQDKNGLDDDLFRLAYHYVYEFSKQHQTKIQNYVALYGFCRNIC
                     LIFIISFWVSVPTFIYRLCTHSDYLYSLLSIMLSFFFVYVFYVGFVKFYRRYTLEVLM
                     AFAVLQSNDTIR"
     gene            18077..18283
                     /locus_tag="LGJ20_003362"
     CDS             18077..18283
                     /locus_tag="LGJ20_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBK2387383.1"
                     /translation="MLNLDCVPISTYCKETGETPEAINKRVQRGVWREGVQVLKVEGV
                     KERWIDLSEVAKWARQNCSNYRAA"
     gene            18244..19416
                     /locus_tag="LGJ20_003363"
     CDS             18244..19416
                     /locus_tag="LGJ20_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBK2387384.1"
                     /translation="MGQTKLLKLPRGVTIRKHRQGETINITFTYKGVRCREPLSNLEV
                     TPKNIKYAERTLGEIHNKIERGTFIYAEYFPRSARLKIFGNAAAGKTVKMYLDEYLEI
                     CETRKLSPSTIGGYKKCRSALASLHICPASELTPATLKAWIQNQKTTLKTIRNQLSFL
                     RSALDEAVTDGVLQINPVSLVTASRYQSDKSEAESSYVVDPLSPAEVDALLAAAGNKQ
                     WENLFRFAIHTGLRSSELCALRWRDIDFVGKTAHVQSASVVGVIKGTKTKAGTRKVEL
                     TEEAMLALINQKPFTFMKDTTVFEDPKTNKPWASADAIRKKAWVPTLRKAGIRYRNPY
                     QTRHTFATRHISRGANLFWLAAQMGHKGPEMLFRHYGSYLKEYDGNTTSNITKKAT"
     gene            complement(19430..19813)
                     /locus_tag="LGJ20_003364"
     CDS             complement(19430..19813)
                     /locus_tag="LGJ20_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020454476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387385.1"
                     /translation="MPVNSQDFLGFAKDCHQRNDEIGYRNAIARSYYGAYHHVLPCMV
                     NGPKDSHQGLINYLQGDASRGSEVYEARYLKGISFILSQLKAQRIVADYRLDQSVTSK
                     QSEVAILTTERLFAKCTEMLKSRAS"
     gene            complement(19803..20618)
                     /locus_tag="LGJ20_003365"
     CDS             complement(19803..20618)
                     /locus_tag="LGJ20_003365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387386.1"
                     /translation="MLQPAKKASVISARLTKYLKDGVRIDDFSFRRMLREIEALRDPV
                     SEDYLLALVYGAHGQVNEAIGFFERSLQVCHNEVVAKNFLVFLSDYGTLKKSFETSIK
                     LAESFISPFIYLQAYENSLFMGKMALAEKYFQSYSKLFGDKEPEKMDNNFDEVVSQVE
                     SFKQRADLSGLEYELIFNNVANVMDSQKVHLSGMRFYNISEEKVNAIVFMTKSSDAEQ
                     IADMNIELAFSMAEHDCLVGKDFAVWFECKDTHIEQNAISELGLITRRVAHAG"
     gene            21283..21859
                     /locus_tag="LGJ20_003366"
                     /pseudo
     CDS             21283..21859
                     /locus_tag="LGJ20_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         5715 a         5621 c         4933 g         5590 t
ORIGIN      
        1 cttcaggctg ccgttcaggt gatacaggtt gcagggcaac atcccgaccg actccgccag
       61 caccctgaca caggaaaaaa ccgccgtcag tcgcatggcc cgctggctgc tgatctgctt
      121 tccggtatag gtgtcgtagg acaacccgat agcatccgcc agctctgctg gcgtggtcac
      181 cggtgcgtca ctttttcgtt gaaataatcc cgaaaagaac actatttacc tccgccgaca
      241 gacgactgtg tacggtcgag atatcgcgcc accagccacg accagaacag acacaacgcc
      301 ccggcaacaa caaaccccgc cgggggataa atcagccagg caccatacgc cagcaaaagc
      361 gcccccagca cgcccaccag aggcgcgaga atcagcatga tcataattac ctcagttaaa
      421 gcgagcggat cccataggac tcaatgtggt cagacaacgt gtcttctttc tcgtacagca
      481 tggctctgcc aaccgccata atcagcgcaa ctgcaccatc gattttgttt tccgcctgct
      541 ctttgacggg cttcaccaca tcatcgttac ccggaatggt tttgccgacc acgttgccga
      601 tacaccaggt catgatggga ttgccatcat gatgaaagcg ccccgattca attgccgctt
      661 ccagctcttt catcgggtcg gacatgttgg tgtagttctg aatgatagtg atggggttca
      721 ggtcttcatc agcaaggtca tgtgacaacc cggtcgcccc gaaggggtcg atgggtgact
      781 cactgaccgg gctgattttg ttcgctgctt tggcctcctc gaggatgtag cgataatcca
      841 cctctgcacc atcggtaacg gtcaggacgc ccatttccac ccatttctga aagcgttcgg
      901 ctgtccgtct atcttcattt ttctcgacgc tgtacaccgt gtcatacggt acccagaagc
      961 gcggggccac actgtagtaa tgcgttttac cgtcaatctc gcgggtataa agtcgcgcca
     1021 tgctgttcat atccagctta cgcgccagat caaaggccag aatgcacggc tgcccctcga
     1081 actgctcaag ggtcagtgat ttatcctcgc agctctgcca gctcaccagg ttgaaatacg
     1141 ccgaacgcgc cgacacccag atattgaggt gttttgtttt aaagacgttt gccagacggg
     1201 cgttattttt cgcacgctgc tgctgactta acaaaaattc gcgataaacc gacacgccaa
     1261 tatttggatt ggctttttcc agcacctgcg ggtcggtcca gtcgtcacct tcatcaacgg
     1321 tatagatgat cccgaacagt tcatcgttag gcaccgagcc gttgagcatc tcgatgactt
     1381 cccgccgctt gtcgtagcac ggcccctcaa tgttgtaccc ggcagtagta atggcccaca
     1441 tcagtggctg acgtcgcgcc cccatcccgg taagcatcgt ggtgtaaagc gcatcggtgg
     1501 cgtgctcgtg atattcatcc accaccgcac agtggggtga tgatccatca ccggggttac
     1561 cgatcagcgg ttcaaaccgc gcgccatcct ccggacggtt catgtttgag gcgttaacct
     1621 caatcccgaa cgcttccgtc agcatgggtg tgcgtttaca catcagtcgc gccgggcgaa
     1681 agacttccca cgcctgtttc tctgtcgtgg caccggaata cacttccgcg ccaaactcgt
     1741 tatcacaggc aaaacaatac agggcgacac cggcagagat tgccgatttg ccgttcttac
     1801 gggggatttc ggtatacacc tcccggaagc ggcgcagccg ggtaccttta ttgacccagc
     1861 caaacgcaca gcagatcaca aagagctgcc acggctccag cgtgatgggc atcctcttga
     1921 atgcccactc ccccttggtg tgcggcaaca gctgaataaa tttcgcggcc cgttcagcca
     1981 ggtccttgtc gaagcggtaa cgaaacgact tacttttttc cgccatcagg tcatcaagat
     2041 ggcgctggca ggcctgaatc acaaactggc aggcaacaat ctttccgcgc acgacatccc
     2101 gggcatactg atttgcagca tttacgttgg ggtaagattt ccggctcatg attcgatgat
     2161 tttcagaaac gggttagtgg ctttcttctt ccccgccagg ccaatcagac gctggcggct
     2221 gctggggtcg agtccgagca ttgcccccgt actgctcatc tcggactcct gttctttttt
     2281 ggcggtcagc tccggatttt tgaccatacc gcccattgca ccggtgatgg tgttgccctg
     2341 tctggcaata tttttcacgg cacgtcgcca gaactcgtag gccacgcacc accgctcaag
     2401 caccgcaagg tcagtcacgc acagcaggcc ctgaccgcag agttctttgg ttgtcagttg
     2461 ccacatgatc gtggcgagag ggagatcttc ttcagcgaac cactccggtg gctcaacacc
     2521 tttgatgggc gtaaaaacag gttcatcttt attcagggct cgcttgccgg ggtttccggc
     2581 cagcgccttg cgcgccgttg gcttggggcg acgcccggaa cgccccgccg ttccagccat
     2641 atgcggcact cctggttaaa tttcattttt cgcgggtata aaaaaacgat ggggcgggca
     2701 gtccggaaga cgtcaggtca cagggatttg acccgcccct cccctctggc tgtgggaact
     2761 ggttcttact tcaaccgttc acgggccgtc ttcgccttat gacatggcca gcacaggctc
     2821 tgcagattac tgtcagcgtc agtgccgcca tgcgccttag ggataatgtg gtcaacggtt
     2881 ttcgcctcac gcaccacacc ggcacgcaga cataactgac acaggccttt gtcacgcttc
     2941 agcacacgtt cacggatggt atcccacttc gaaccgtaac cgcgctgatg acgtgacagg
     3001 cctggtttgt attgcttcca gccttcgctt ttgtggcttt cgcaatagcc tgacgggtca
     3061 gtagtggtat tgcggcaacc gcgaacgcgg caggcttttg gtgtacgtgg tggcattaat
     3121 tgtttcctta agccattacg atgcgtctga aaatgagtta atctgaaaat tccttcacta
     3181 tgcgagagca tgacaatggt cataggacaa gaagatcaga aatgctgccc ggcttgcaat
     3241 tcagatgcga cctggcagaa ccgtgatacc gcttggttaa ttagatgccc gatgtgtgaa
     3301 acatttctca tcaggaattc caccattgaa attttgcgaa gtgacgttgt gtatcggaca
     3361 ttagctggtg accttctcaa acaagaaggt gggtgtgact acatgcttac aagaggaagg
     3421 cttgcgaact tcgcgaaaac ccagctccca aaatcaaagt ttcaggaata cttccctggt
     3481 gacaactacg aataagccat tacaaagccc acccgcagat gagctttgta atggctactg
     3541 tgagttgcgt gacgtttcta tcttcctgat actggccttg tcgatgttgc actggcccag
     3601 cgccgaaagc aggctcacat tcagatccag actggcccca taggtcagcg ggtcgggaat
     3661 gactggctgg ggcgtttcag tggtcaggct tgttggcagc ggtaccgccg gaaccggcac
     3721 gtaaactgtt cgcgtacttc cgcaaccggt cagcagcggc agcaggcaca tgacgtgaag
     3781 cacaatcatc atccgcaaca gccactttga tatcttcctg ggttctctgt gactccagtg
     3841 cgatctgctg ttttgcatgc tggttagcct ctataactgt attgatgatt tgcagtgatt
     3901 gcagaacgtt acgggtaatg gcagttgctg attcagcatt tcgtacagcc tcatcagcac
     3961 gctccttttc gtgctgatat ttgctgtagt aatgtccagc agaccagatg aaggaaccaa
     4021 tgacgctaac aacgaaggca acaataacca gcttatatct cagcttcatt tactacccca
     4081 ccagcttttt taaatcgggc aatcaggtca ccgattttat gttcatactg accgtaacct
     4141 gcaccaggta acgacgccca gatattgctg caacggtcga ttgcctgacg aatactgccg
     4201 cggtcaatca tcggtaaagc accacgctct ttaatctgct gcagagctac agcgtcctgg
     4261 ctttctggag aaaaatcttt caggccaagc tgtttacggt aagcatccca ccagcgtgaa
     4321 agaagctggt aacgtccggc agctgttgat ttgagtttgg ggtttagcgt gacaagtttg
     4381 cgagggtgat cggagtaatc agtgaacagt tcgccaccaa caataacatc ataaccgtgg
     4441 ttacgtgtcg gttgtcgccc gttatccgtt ccttctgacc atgccaccat atccaggaaa
     4501 gctttacgct gggaatttag tgcctgcata aattactcct tcgagctacc aaatttgtta
     4561 ccgattactc gcattgcagc cccacgaata gcatcgacac cgatcagccc cacgccacca
     4621 ccaatggcaa cagaaagcga tttaggccat ccgacatatt caagagcgga tgcaaaggtc
     4681 agcgtcagag cgccacatag caaaatctcg agcgtttttc gcttccagcc accaccaccg
     4741 ccaaaatagg cgatgcgcaa accagccata acgatcgaca taatcactgc acccagcggt
     4801 gtgtctccac gccaccagct ctgaaacaac tccagccagt ccggccaggt atttgggtta
     4861 tgaggcattt cgtcatctct cacctcgcga tatttgcggg tgctgtgttg gaaataaaaa
     4921 ggccacgcaa cgtggccacc agaattattt ccccaccagt tcacttacct ctttcaccgt
     4981 ctgattaaac cgctctgact caagttcaac acctaacgcc cgacgcccca gcgccattgc
     5041 tgcttttatt gtggaaccgg atcccataaa gaaatcagca accagatcac caggtcgact
     5101 actggcattg attatttgcc ggagcatatc cgccggtttc tcgcacggat gtttacccgg
     5161 gtagaactga acgggcttat gcgtccagac atcggtataa ggcacggaga ctgatacgga
     5221 gaaatagcgc cggagagatt taaactcatc cagcaattca gaatatttgc gattcagtga
     5281 atcataagat gccaccagct ggtggtgtgg ttgttccagt tgttgttcct gaaacttctc
     5341 tgccgctata cgggaaaaca gtgcctgtaa cttccgatag tcagcctcat tcggcaactg
     5401 ccactgactg gcaccaaacc agtgggaaac catatttttc ttacctgtgg cttcggcaat
     5461 ttgttttgcc gttataccca gttcggcacg agcatccctg aaatacgata tcagcggtgc
     5521 cattatgtgc tgtttgagtt ccctttcttt tgccgcatag ccgtcacttt tgccgcgata
     5581 tggcccctgg taatgttcag caaacagaac gcgctctgtg gcaggaaaat atgcgcgcag
     5641 actttcttta ttacacccat tccaacgtcc ggacggcttc gcccagatga tatggttaag
     5701 cacgttgaaa cgttcacgca tcatgatctc aatatcagat gccaggcgat gcccacagaa
     5761 caggtaaagg cttccggcag gtttcaacac ccgccagaac tgggccagac agtggtccag
     5821 ccacttaagg taatcttcgt cccctttcca ctgattgtcc caaccgttag gtttcacctt
     5881 gaagtaaggc ggatcggtaa caatcaggtc aatggaatca tcaggcaggg actgaataaa
     5941 atgcaggcaa tcagcgttga ttaaatcaac actgtttatt tttacagtat ttttcatgga
     6001 tcagtaagcg taactctggt aggctcactc tgcttttgcg ctaaagcagt gggccgtggt
     6061 tcgcttgtga ccagtaagca tgagcgaatg gctggcaggt gctaccaaca cccaccagcc
     6121 gcccattttc acagcaggaa accgccatta ctggcagcgt ctgaatttat tcccgtaccc
     6181 gccgttatcc ttcgccagac ccgccagaac taactgagtc agtattaact ggcaccgggc
     6241 ttcgcttact ccggtagttc tcgtcatcat gcgtggcgtt acccacttgt cagcaggtaa
     6301 gaaatgaagg actgcggcgg cggtttctgt catatcttgc tgttttagca tgtctttttc
     6361 ccttctggtt aacatgacat accaataact cttgtctaaa aagccagcaa gataaaaagt
     6421 cagtattcac gaccaccagc gtgtttactg tactgcacca agtttacagg tacaaaaaac
     6481 ccgctcagtg gcgggttctt aaatcttatc aacggtagac atacaaagcc catcgttggg
     6541 aaaatcttat ccatgttttt tgaaaaatgc aagcatcatg tcgccatctt cggcgaaaat
     6601 catttatttt gtcacttttc tcaattgtgt ctctgcatat gcttcttcct gccagcactt
     6661 tgtaaccagt ttatcaatga catctgcata tcctttgtac cactgataat ccgtcagatc
     6721 cggtaccagc ttctggacat gatgccgcgc cagtgtggtt ggtaaacggc taaaccggtt
     6781 tccattgcaa cgcccacaaa tcttataaac aggcgtgcca tgaagccggg tccttttttc
     6841 atccaggaca atacctttac ccttacaccc tctgcacgct gtgctgactt ctcccttacc
     6901 atgacaatgc tgacatagtt ccttcaccca ctcctccttg ataacagatt caccgcttct
     6961 ggagtgtttc accacttcgc gcaatacatt atgaaatcca gtacctgcac aatgctcaca
     7021 gcgagcctta cttgccgcag acctggaata atcagcaaag gcaaaattca caaggtaagg
     7081 gatgatctgt aaccgggttt cttcactcaa tttattcaat gtcgggttat ccagtgccat
     7141 cgcgtaattg agtagacctt caatcgcaaa ctgaggatcc tgaacaccaa cttttgccag
     7201 gaataaggca aaacccagtg gtgctttcga ctgcaccatc ccctgcgcag ccatcacatc
     7261 cgtaatcgtt aaaccaccag agcctgtcgc cggtgcgtca tcgctcaatt ttggagattt
     7321 tggggagtaa tattttggta aggcttcaag gttcatgctc attctccact tacgccagta
     7381 cgcctattgc cagcgcacga tcgataaaac gaaatatcag ctccagctgg gagccatact
     7441 tctcttcaaa tgccacggta tccgcatgca gctcgtcgtg atgctttctg cacaaaggca
     7501 acacaaagag gtcatgcgct tttgtaccca ttcccccctg accgtggcct atcaggtggt
     7561 ggggatcatc agcgggcttt ccacaacatg cacacggctg tgtcttaacc cagcgcgtgt
     7621 acttttcatt aacccagcgg cgacgttttg ggcgtaacat aaaagactcc ggcgactccg
     7681 gatccacttt cagcgccagc accttttttg ccttatcctg gatgatgctg gtggcaggaa
     7741 ccgaaggcac aaggtcactt tcccgggtga cagacggcac aacaggcttt ggtaatctca
     7801 gtgccttacg ggctgcactt tccggtaagg catccgccag gtcattacga atcagccacc
     7861 agcacagttc cggcattgtc acaacgtgac tatcatcaaa accgagatcc cgacgcacaa
     7921 cagacaacac ccagcgggca cagttatccg ttgccattga ttccagccgt tccgtgaact
     7981 gatcgcgcag ctggttatca cagtgccagc acagacggat tgcgcccgga gcgtgtcgca
     8041 ttgtggtcat gttctcgctg tgccagtcgg aatgaggcca ctggcagcct ttttcacgaa
     8101 gtaaccagct ttcaagacat tccacgccac cagcacgacg gatcactgcc tcattgcgga
     8161 acacggcccg aacggcagga tcatccgcca gcggttgtga tgccgccgga acggcaccac
     8221 tggagaaaga tgaataacgc tccggctcag gctccagcag gacacgcccc tgcataaaca
     8281 ggggcatcag ctctgaaccg ggtctgaaca atacgatccc catacgcggg gctatttcag
     8341 gggtcagtag tgctctcacg gtcacctcaa tgaacggtat cgagcagctt taacagctca
     8401 gggaatcggg attcgaagaa atgcggctgc gtctcacgcg gatttgcggg actggtaatg
     8461 ttcttgccga acatgcaacc tttcgctgtc agcgaccaga attttttgat gttgttaatc
     8521 gcggtacggc tgtatcgttc gcgttgttca acgatcccca gcttcgccat ctggtgatat
     8581 gcctgattag ccgtcaggcg gataccatac tgcttcagca gtgcactcag cgacagcgtc
     8641 gggcggcttg agccatcagg cgcgtcagca ggtgcatcaa tggcatagcg cggtgccaga
     8701 ttcggtaagc caacagcctc ctggagtttc tgacaggccc caagcactga agagttagac
     8761 aggtttaact ccctgcgcat aaagtccagc agaatcactc cagcctgcat cttgtcagca
     8821 gcctgcccgg ataatttttc cggtacgctg gttaccatat cgaaagtacg gatcaccttc
     8881 agatggaatg acgggctgat ccacattgca taggcataca ccagttcttt gcagacatac
     8941 gtcccctggt tatttccgcc attaatgacg ctaactggtt gattttgttc cagaggcgga
     9001 attccaccct cggtgaaaag ttgttcaatc aattcacagg tttgcttatt ggagagccag
     9061 tatttcgggc ggtttttttg ttctcccccg gctgccctgt gcagatcgtt caggctgtaa
     9121 cgcccataag catcacgacg aacttcaata ccatcaatga ccatcagatt attcatactt
     9181 cgtttctcct cttaatcagg cggctgcacc cgccgttttc tcgtacttac tgatagtgat
     9241 ctcgaccttc cctcccggga taaccggtcc ccactccacc agcattcttt tcacctgact
     9301 gtcgtcttcc cacacacccg cgtgggtcag ggcgtcaaac agcgccttgt tatagttgtc
     9361 cagatcgcgg atccggttat ccggaggaaa caacacgatc tccactgaag caggtgccga
     9421 cgttggtttc ggcagacgac gtaactgctc aactattgct gcgcacgccg cgctctggaa
     9481 ttttcgcccc gccgcgctta tcagactctt accagcaaac gcccctttgt tggggtgtcg
     9541 ccagtacgtg ttcacgctgg gcgggaaagg caagatcagc ttcatactta caggcctctc
     9601 tcatgtaacc agtgggctgc acgcagcctg gcgttttcct caccggcaag cagtgtgcgg
     9661 ataatcccga ccgcctcgct gtcgtcgtcc ttcaccgcgg tatgaagcgt tatcccccgg
     9721 gccacgccac gctttatcgt gatgacgcct tttttctcca gtgcacgaag atgctctacc
     9781 gctgcattca ctgaacggta tcccagcatg gttgccacct cctgattggt tggcggaaag
     9841 ccacgctctt tctggtaaga aatcagcata tccagcacct gctgctggca ttgagttaac
     9901 gtcgtcatgc cgccatctcc ctgaccagtt tttccgcctg ctggcgaacc tgcaccagaa
     9961 aggcttcacc acatgcctca agttcatcgc gcccgatgta gctgattgcc ggtcccttcc
    10021 aggtcttgtc gaaaacagca atagcaccag cgaagaaagc gcctgtcggc acctgcttct
    10081 catccttcgg gataaaccag gcaggcagtt caaaaccaat acgcccgcga ataaaagcaa
    10141 tatggtccgc atcttccggc caccacactt cgctggtggc agctttgatc aggaaaacat
    10201 agcgcccgcc cttatcacgc atggcactgg catgcttcat gatgtaacgc atgccggtga
    10261 tgtattgccc ctcatgctga ctggcgcggc tgtatggggg attaccaaag gcagcacctt
    10321 taagctccgc aagacgttct gaccagtcat gcgccagcgc gttgtcttcc gccgtgtaat
    10381 acgcagcaca tttggcgtta tcaccgtcag tgaacagatc cagaacaaac gggccaaaca
    10441 gggtgttaat tccccagaaa atgttgtccg gcgtgcgcca ctgatcgccc acgtccttca
    10501 gttcatgggc tggtttgttc cgcagttcca ccagcgcctg gcaatattta ttactcatta
    10561 agcccccacg taattccctg acagatacca ctcttcaccc gatgcagcgc gcttgctgct
    10621 tttccgtaag caccgctcac gacgcgccag aaaattgttt cgttctggct gggagtggct
    10681 ttcacggaat gccgccatcc acacggttgc agcacgacgg tataagcctc ttgactccag
    10741 ttcttcagcc tggcgggtca ggcacaaaat cacccgggga tcgttagtgc cgatatagaa
    10801 attgcgcaca ggtctggttt cacgaactgg ttgtggttcc ggctcctgcg ctctctcagt
    10861 caggcgcggg aaatgtctgc gtgtatcccc ttcacaacgg tgagccacac gcccactctg
    10921 acgtaacttg cttgctgact gcagtacgcg ctgccgtgag taacctgcaa aagcatccgc
    10981 aatgtctccg gaagtacacc ccggatgggc ttcaatgaat ttctgaacgt cattcaaaag
    11041 actcatgatc accccctgaa tcctgccggg atctggctgt agtccacgtt gtcgtaactg
    11101 gatttgaagt acgggtcctc acgtctggct gcagataccg caggaacttc ccaggattct
    11161 tcgaaatgac gatccggacc aaagaacgtg acagcctgtt tcacaaattg tgtgccgctg
    11221 ttacccatcg cagataccca gcccgcatag cgtttcacac cttccagcat ggtttcgggg
    11281 tttaccccct cattcaaacg ggctttccag gctttgaagg ctgcagattt tgaattgcca
    11341 ccagcacgtt tgggatatgc cagccatgcc tgctcaaact ccggagaata ttccggtcgg
    11401 tttgaacgaa ctcgcacgga ctcatcaact gatgcaccaa cagctattgg ttcattgact
    11461 ggttctttga ctggttcaaa agagtgactg gttctgggtg aatctcctgc actaccccct
    11521 ggtgcaactc ctgcactacc tggtgaattt gctgcaccag atagtgaatt atttgcacta
    11581 ccccctggtg aatctcctgc accatccaga tgaaggagat agatattact tgagttacct
    11641 ttttcacctt tccgggtgac tttttttacc agcccggact cacaaagggc cgcaatatga
    11701 ttcatcacag aacgtttgct aatctcgcac tggtcagcaa tatgctggta gctgggccag
    11761 cactcaccct gatcgctggc attatcagcc agcttgatca gaaccagttt tcgcaatgga
    11821 ttacccactc gaattttcat cgctttaacc atcagctcca tactcatgct gcacctccga
    11881 gatgcttcat gttttttccg gagcgaaagg ctataagcgg catactgacg cggtaattac
    11941 ggcccagcgg ttcacaaatc accttctggc attcacggtc aaccaggcta acacgtagaa
    12001 catgccctgc aggtgtggtg taccactgcc caactgtagg aattgatgtt tttttacgct
    12061 gaagcaaacg gcaaatattg aggatcaacg gattaagcat gacgatgccc tccgctgata
    12121 ttcaggagac ggtgaatatg aaaattagcc ttatccgcca gacgaatacg ttcagcctgc
    12181 aagttaagaa gggtttctac cagaacttga tgcgcctgcg gatctgaaag agttaccttg
    12241 cgcagagcac gtagtgctgt tgttacataa ctgagtttat gtaagtcttc atcattcaga
    12301 cgagtgaggg ctgggacagt agccatgatg gcagcctcct tgatcggtga aatacttcca
    12361 ccaccggaaa cgccaatttc gctggtggtg aactgaacgg ggttggcgta accggtgatc
    12421 aaggaaaccg gcgcatcttt cgatgccccc gcccagccca ccataacttt gatgtgagca
    12481 aatgcggacg ataaaaaaga cgctggcgcg tcatatatcg ccttgatcaa ttccaggacg
    12541 ccaatcccgg cacccgcttt ataaggtgcc tgaacagtgt aacgtcccgg aatggcagaa
    12601 tcaatgtgct ggtggtcctt cacactcaat aaaatcacgc ctgaatttcc acaaaggact
    12661 aaagcactca tgcgggtagt ctttgcgaag atagataacg cgctgtgttt ctggctccca
    12721 acgaataaca tgaacataaa gtcctcttcc gtcacgaaac cagcggttaa gttcctgcac
    12781 aactcgcccc ccacagtcag gtaaagttct ctgtggttac ttacagccag gtgatttggt
    12841 aatctgcatt catgccgtaa caacaggtgt tcagcgacgc tgaccaccag ctgttgcgac
    12901 aaacggttat ttgccgttaa actgttcatg cgttagtttc tccacagaca caaaacgcca
    12961 cgacgcccgg agctgcacac tcgcgggcgt cactcttttc tggagcgcaa aagattttgt
    13021 agaccagtgc tgcatgctcc tggagcttcg aaattgacag atacaactca tcattaattg
    13081 ctgtctgctc gtgtggctcc acgaccccat cttcgattgc cgaacgaatc tgctttgagt
    13141 aattcccgat ctgttcgatg acttccagca ggcgctggtt tatatcggcg ttctctactt
    13201 cctcaatttc aggaagcgat acgaacaccc caccagcaga ctgtgcgaca gcatccgcaa
    13261 tgtagtgagt gccagccgcg cgctgtaaaa ccattgccca tcccagcggg aaaatctgat
    13321 cgccatcggc acgaaggcgg ttaaataatg cgttctctgt tacatccagc cagtcagctg
    13381 cttcagcgta accacccggc aacgctgcga tagtttttct gacagctttc acgtaccact
    13441 caggctgttt ttctactttc cagtgatact tacccacggt tagcctcatc gttctgtggt
    13501 taaaaattga aggtgttctg ttaatctttc ggatagatat ccggtcttaa gtcagatttc
    13561 gtaattgcac ctgacgtgca ttgctcaagt tttttcgcca gcacaaaact ggctttttta
    13621 taaccattga aaaccagccg taagtagcca ggtgttgagc caacttttcc ggccaactcg
    13681 ccctgctgtt ctttggttaa agagtcccaa tacgctttca tacaatatgt acctccggta
    13741 tacatattac atgattgaaa tgaaccttca agatacttgt accttatcgg tacaaaggtt
    13801 ttaatttcgt tatgaaaaca atccatgaca tccggcggtc taacgccaga aaactgagag
    13861 atggtgttgg cggaaattca tcctttgcca ccatgattga tcgcgagcca acccaaacca
    13921 gcaggtttat gggggatggc gctactaaaa atatcggtga cagcatggca cggcacatcg
    13981 aaaaatgttt cgacctgcct gtcggatggc ttgatcaaga acaccagaca acgaacatca
    14041 caaaaaaacc tgatgtttca atcactaaca aacaaataac gttagtccct gtcatatcat
    14101 gggtacaggc cggagcatgg aaagaagttg gctattctga ggttgatttg agcacagcag
    14161 aaacgtatcc ctgccctgta ccctgtggcg aaatgactta tatcttgcgg gtgattggtg
    14221 attcaatgat tgatgagtac cgccctggag acatgatttt tgttgatccc gaagtccctg
    14281 cctgccacgg tgacgacgtt attgcattga tgcacgatac aggcgaaacc accttcaagc
    14341 gattgataga agatggaaca cagcgttatc tcaaagcatt aaacccaaac tggcctgagc
    14401 cttacattaa gattaacggt aattgctcta taattggtac agtgattttc tcgggaaaac
    14461 caagaagata cacaataaag gcctaatcaa tatttatgaa cctgcttcgg caggtttttt
    14521 tatacttgac aatgtaccca tgagatacat aatgtatcta aaagaaacat aacacaggca
    14581 agattaaaac aaaatttggt tgtaacacgg cgtatggcac atgcgtcgtt agcggtctgg
    14641 tgacgttaaa ggggacaatc cactccttgc tcgagcaaac aaaccaggta gccggaatgt
    14701 gcaagtcaat gatgatgctg ataagacgcc taaccagcgt ggcgattcgg tttgacgcct
    14761 gggaagagac cagggtgcaa cgatgagggc atttatggag ccgcgacaaa gtgtggtgcc
    14821 gtaactggct aagtgctctc agcgttgtgg taatccgcga aatggcgcgg cggtaagtat
    14881 ggcggggtta ctctttcccc gttgaggaca ccggattgtc aggttgacca tacgcctgag
    14941 tgacaacccc accacaacag ccactgcttt ggcggtacca gtttgtaccc ttgcttccgg
    15001 ctggtaccgc tctttttaca aaacagagaa gagcatcacc ggacgacggg ctcataaccc
    15061 aatccatccg ggcggcagtc accgcaggtg ttcttctctg ttttgtggag aaaccaaccg
    15121 accttgcagg gtcgatatga tgaggagcag caaaatggct agcgaacgca gtactgatgt
    15181 gcaggcattt atcggggagc tggacggcgg cgtatttgaa accaaaatcg gcgctgttct
    15241 cagtgaagtc gcttccggtg tgatgaacac gaaaaccaaa ggtaaggtct cgctcaacct
    15301 ggaaatcgaa ccgtttgatg agaaccgtgt gaaaatcaaa cacaaactct catatgttcg
    15361 cccgactaac cgcgggaaaa tttctgaaga agacaccacc gaaacaccga tgtatgtcaa
    15421 tcgcggtggt cgcctgacta ttctgcagga agaccaggga caattactga ctcttgccgg
    15481 tgaacctgac ggaaaacttc gcgcagcagg tcattaatat cgttcttaat taactgatta
    15541 tttatctcat cactgaatat ctttatatag tgaggactta ttatgtctca gaacttagac
    15601 gcaaccgcaa ttaatcaaat ccatgccctt atttctgctc agggtgttaa tgaaattatc
    15661 agtaagattg gtgccgatgc tgtggcattg cctgagaatt tccgcattca tgatctggaa
    15721 aaatttaatt taaatcgctt ccgtttccgt ggtgcgcttt ccactgccag catcgatgac
    15781 tttacccgtt attctaaaga tcttgcagat gaaggcaccc gctgctttat cgatgctgat
    15841 aatatgcgtg ccgtcagtgt gcttaacctg ggtactattg atgaaccagg tcacgcagat
    15901 aacaccgcca cactcaaact gaaaaagaca gcaccgttct ctgctctgtt gtctgttaac
    15961 ggcgagcgta actcccagaa gtcactggca gaatggatcg aagactgggc cgactacctt
    16021 gtgggctttg atgctaatgg tgacaccatt caggcaacaa aagcggctgc ggcggtccgt
    16081 aaaatcacga ttgaagcaaa ccagactgct gattttgaag ataatgactt cagcggcaaa
    16141 cgctccctga tggagtctgt cgaagcgaag accaaagaca ttatgccagt ggcatttgag
    16201 tttaaatgcg ttccgtttga aggtctgaaa gaacgtccgt ttaaattacg cctcagcatt
    16261 atcactggcg atcgtcctgt actggttctg cgcattattc agctggaagc ggtgcaggaa
    16321 gatatggcta acgaatttcg tgatctgctt gttgagaaat tcaaggacag caaagtagaa
    16381 acctttattg ggactttcac cgcctgattt cattactgca aatgcccctg cgggggcatt
    16441 tatggaaacg taattgactc aataatcgcc tgaaggcgag ggttttcttt aaccaaaatt
    16501 cagcgcggtg cagcgcatat aacgtggaga acaaaatgtc atttattaaa actttttccg
    16561 ggaaacattt ttattatgac aggataaata aagacaacat cgatattaac gatatcgcgg
    16621 tttccctttc aaatatctgt cgctttgccg gccatctttc acacttctac agcgtcgccc
    16681 aacatgcggt gctttgcagc cagctggtac cgcaggaatt tgcttttgaa gcgttaatgc
    16741 atgatgcaac agaagcgtat tgccaggaca tcccggctcc actgaaacgc cttcttcctg
    16801 actataaacg gatggaagaa aaaatagacg ccgtaatccg tgagaaatac gggttacccc
    16861 cggttatgag tacgcccgtg aaatatgccg atctcatcat gctggcaacc gaacgccgcg
    16921 atctcgggct tgatgatggc tctttctggc ccgtactgga aggtatcccg gcaacagaga
    16981 tgttcaaagt tattccactg tcgccaggcc atacctatgg gatgtttatg gaacgcttta
    17041 aagagttaac tgagtcgtaa aaatcagcac gtacgaattc aaactctgcc ataaaagaac
    17101 atataagtaa tttattaaca tatagatata ggttatatta caaattgaaa aattattgga
    17161 gagcaacaat gaatcaaaat ccattctcat tctatgactt tcttggatat ttaataccag
    17221 gtggtttttt tatcctatta atgtatttct gtggtttgac attcgatcta gatattgtta
    17281 ttgacttaag tgaattgctc agaggtcaaa gtcaaatctt tggcattttg aactacgctt
    17341 caatagttat tatatcttat atagctggac attttatttc tatcacgtca gcatttttta
    17401 tcgaaaaata catgaataaa gaattaggat atccttccaa atatctattt aaaaaattga
    17461 tagatacctc agaaagcatt tgtagcccat catgcgatga gagaagcgct gataaaaaaa
    17521 caaaaataaa gaatcgcata atcaaatgcg tattatgtcc aataatacta tgggatttta
    17581 taacacaaaa actatgttat tctcaatctc taccatttca tttagctaat acaacatggt
    17641 taatgatcaa agaaggttat gagaaaaagt tcataacaaa tcgccaatta ttacaagata
    17701 aaaacggact tgatgatgat ctcttcagat tggcatatca ttatgtttat gagttttcaa
    17761 aacaacatca aacaaaaatt caaaactatg ttgcattata tggtttttgc agaaacatat
    17821 gtttaatctt tataatttca ttttgggtat cagttccaac ctttatttat cgattatgta
    17881 ctcatagtga ttatctttat agtttacttt caataatgct tagctttttc ttcgtctatg
    17941 ttttctatgt tggttttgtt aaattttata gaagatatac tttagaagta ttaatggcat
    18001 ttgctgtact tcaaagtaac gacactattc gttaataatg ttgctcccgt gtgcagacgg
    18061 gataatggag aaacgtatgc tgaacctcga ttgtgttcct atttcaactt attgcaaaga
    18121 aactggcgaa actcctgaag caataaacaa acgtgtacag cgcggtgttt ggcgtgaagg
    18181 tgttcaggtt ttaaaggttg aaggcgttaa ggagaggtgg attgatctta gtgaggttgc
    18241 aaaatgggcc agacaaaact gctcaaacta ccgcgcggcg taacaatcag gaaacaccgc
    18301 cagggagaaa cgatcaatat aactttcacc tacaaaggag ttagatgtcg tgagccgctt
    18361 tccaatctgg aagtaacacc aaagaacatt aaatacgccg agcgtacact cggcgaaatc
    18421 cataataaga tcgaaagggg aacattcatt tatgcagaat attttccccg ttctgctcgt
    18481 ttgaaaattt ttggtaatgc tgctgcaggc aaaacggtaa agatgtacct ggacgaatat
    18541 cttgaaatct gcgaaacaag aaaactttca ccctctacga ttggtggcta taaaaaatgc
    18601 cgtagtgcgt tagcctcact tcacatttgc cctgcaagtg aattaacacc agcaaccctg
    18661 aaagcgtgga ttcaaaatca gaaaacgacc ttaaaaacaa ttcgcaacca gttatctttc
    18721 ctgcggtcag cacttgatga agccgtgacc gatggggtac ttcaaattaa ccccgtatcg
    18781 ttagtaacag cttcgcgcta ccaaagtgat aagtcagaag cagaaagcag ctacgtagtt
    18841 gatccgctat caccagcaga agttgatgca ttactagcag cagccggaaa caaacaatgg
    18901 gaaaatctgt tccggttcgc tatacataca ggcctgcgta gttctgaatt atgtgccctt
    18961 cgatggcgtg atatcgactt tgttggaaaa actgcccatg tccagagcgc aagtgttgtc
    19021 ggtgttatca aagggacaaa gacaaaagca ggtactcgga aagttgaact gacagaagag
    19081 gcaatgttgg cgctgataaa tcagaagcca tttacattca tgaaggatac tactgtcttt
    19141 gaagatccaa agaccaataa gccttgggca agtgctgatg caatcaggaa aaaagcatgg
    19201 gtgccaacat tgcgaaaagc aggtattcgt tacagaaatc catatcaaac taggcataca
    19261 ttcgccaccc gccatatcag ccggggagca aacctgtttt ggcttgcagc tcaaatgggg
    19321 cataaagggc cggaaatgtt atttcgtcat tatggttcat atttaaagga atatgacggt
    19381 aatacgacca gcaatatcac aaaaaaagcc acttaagtgg ctttatcaat taggaggctc
    19441 tcgatttaag catttccgta cacttagcaa agagcctctc agttgttaga atcgcaactt
    19501 cagactgttt agaagtgacc gattgatcaa gtcgatagtc tgcaacaatc ctctgagctt
    19561 taagttgact caggataaaa ctaatccctt ttagatatct agcttcatat acttcacttc
    19621 ctcttgaagc atcgccctgc aaataattga ttagcccctg atgactgtct ttcggcccgt
    19681 ttaccataca aggcaagaca tgatggtaag cgccatagta ggatcttgct atagcatttc
    19741 gatagccgat ttcgtcatta cgttgatgac agtcttttgc aaaaccgaga aaatcttgac
    19801 tattaaccgg catgtgcaac cctccgggta atcagaccta attcggagat agcattctgc
    19861 tcaatatgtg tgtctttaca ttcaaaccaa acagcaaaat ctttaccgac taaacaatcg
    19921 tgctcagcca ttgaaaaagc aagctcaata ttcatatcag cgatttgttc agcatcagaa
    19981 gactttgtca tgaatacaat cgcattcact ttctcttccg aaatattata aaaacgcatt
    20041 cccgaaagat gaaccttctg agaatccatg acattggcta cattattgaa aataagttca
    20101 tattctaaac cagaaagatc ggcacgttgc ttaaaggatt cgacctggga aacaacttca
    20161 tcaaaattgt tgtccatttt ttcaggctcc ttatctccga acaatttaga gtatgactgg
    20221 aaatacttct ccgccaacgc catcttcccc ataaagaggg agttttcata agcttgaaga
    20281 taaataaacg gggatatgaa gctttcagca agcttaatgc ttgtttcaaa gcttttcttc
    20341 aacgtaccgt aatctgacaa gaaaacaaga aaattctttg caacaacctc attatggcat
    20401 acctgcaagg aacgctcaaa gaagcctatc gcctcattca cttggccatg tgcaccatag
    20461 accaacgcta aaagatagtc ctcagagaca ggatctctaa gtgcttcaat ctcacgtaac
    20521 atccttctaa agctaaagtc atcaatcctc actccatctt tgaggtattt agttagccgc
    20581 gcgctaataa ctgatgcttt ttttgctggc tgcaacatta aaaaccttat cttacaaata
    20641 gttggtaatg atgttgaatt attttttgtt ctttatggct aataagatta catgaaggga
    20701 caacttctga ccaccttaga aaacactcac tttagtaaca agagtcaata atattgttga
    20761 caaatcacaa aaaaacaaac ttatccaaaa caatctggtt tatcacaaag cctctgtgca
    20821 taaatctgtg cataacatca tttttacaca cattttttta taaaaatatt ttttagcaaa
    20881 aaatgctatc tatctccgaa actgcaacca caaaccatta attccaaatg aaactatttt
    20941 aaaataaata gtaagacagt aacaggacag aaaaagttcc gcaaaagagc cgcttgtaaa
    21001 atcaacaata tttggctatt taaattcaat gaattacata actatggaca cgggttcaac
    21061 tcccgccagc tccaccaaaa ttctccatcg gtgattacca gagtcatccg atgaagtcct
    21121 aagagcccgc acggcgcaag ccctgcgggc ttttttgtgc cctcaatttg tcccgcgaag
    21181 tccgaagaaa actaattaaa tccgatcctt ttaggcacct tgttaggcac ctcataaagc
    21241 tttattgttt tttgaggtgc ctaaaactat ggaaacccgg caatggcaag acaaaccaaa
    21301 cctctatccg ttaaagaaat agaatcagcc aaacccaagg aggcggacta cgttctctat
    21361 gatggcgatg gccttgagct actcattaaa tccagcggga gtaaaatctg gtagtttcgc
    21421 tacattcgcc ctgtcaccaa gaaacgagca aagaagagca taggctccta cccgtcagtt
    21481 acccttgccg atgccagaaa ctaccgtgca gagtcccgct cactactggc taaacagatc
    21541 gatccgcagg aacatcagca agaacaattg cgcagttcgc tggaagccaa aaccaacact
    21601 ttccaactcg tagctgaacg ttggtggaat gtgaagaaag ccagtgtgac agaggactat
    21661 gcggaagata tctggcgctc tcttgaaaga gacgtctttc ctgcgattgg cgacgttagc
    21721 gttacagata ttaaagctca tacactggtt caggccgtac aaccggttca ggccagagga
    21781 gcactggaaa ctgttcgtcg cctgtgccaa cgtattaatg aggtcatgat ctatgcccag
    21841 aacacagggc tgattgatg
//
LOCUS       DAFATA010000077        21227 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFATA010000077 DAFATA010000000
VERSION     DAFATA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.078"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1212
                     /gene="dtpB"
                     /locus_tag="LGJ20_003367"
     CDS             <1..1212
                     /gene="dtpB"
                     /locus_tag="LGJ20_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBK2387387.1"
                     /translation="GALVLAIGYFMTGMSLLKPDLIFIALGTIAVGNGLFKANPASLL
                     SKCYPPKDPRLDGAFTLFYMSINIGSLIALSLAPVIADRFGYSVTYNLCGAGLIIALL
                     VYIACRGMVKDIGSEPDFKPMSFSKLLYVLLGSVVMIFVCAWLMHNVEVANLVLIVLS
                     IVVTIIFFRQAFKLDKTGRNKMFVAFVLMLEAVVFYILYAQMPTSLNFFAINNVHHEI
                     LGFSINPVSFQALNPFWVVLASPILAGIYTHLGNKGKDLSMPMKFTLGMFMCSLGFLT
                     AAAAGMWFADAQGLTSPWFIVLVYLFQSLGELFISALGLAMIAALVPQHLMGFILGMW
                     FLTQAAAFLLGGYVATFTAVPDNITDPLETLPVYTNVFGKIGLVTLGVAVVMLLMVPW
                     LKRMIATPESH"
     gene            complement(1261..2013)
                     /gene="rsmJ"
                     /locus_tag="LGJ20_003368"
     CDS             complement(1261..2013)
                     /gene="rsmJ"
                     /locus_tag="LGJ20_003368"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBK2387388.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(2021..4063)
                     /gene="prlC"
                     /locus_tag="LGJ20_003369"
     CDS             complement(2021..4063)
                     /gene="prlC"
                     /locus_tag="LGJ20_003369"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBK2387389.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            4266..5108
                     /gene="rlmJ"
                     /locus_tag="LGJ20_003370"
     CDS             4266..5108
                     /gene="rlmJ"
                     /locus_tag="LGJ20_003370"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBK2387390.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5180..6532
                     /gene="gorA"
                     /locus_tag="LGJ20_003371"
     CDS             5180..6532
                     /gene="gorA"
                     /locus_tag="LGJ20_003371"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK2387391.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6586..6669)
                     /locus_tag="LGJ20_003372"
     CDS             complement(6586..6669)
                     /locus_tag="LGJ20_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBK2387392.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            7409..7762
                     /gene="arsR"
                     /locus_tag="LGJ20_003373"
     CDS             7409..7762
                     /gene="arsR"
                     /locus_tag="LGJ20_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBK2387393.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            7816..9105
                     /gene="arsB"
                     /locus_tag="LGJ20_003374"
     CDS             7816..9105
                     /gene="arsB"
                     /locus_tag="LGJ20_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBK2387394.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            9118..9543
                     /gene="arsC"
                     /locus_tag="LGJ20_003375"
     CDS             9118..9543
                     /gene="arsC"
                     /locus_tag="LGJ20_003375"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBK2387395.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(9677..9973)
                     /locus_tag="LGJ20_003376"
     CDS             complement(9677..9973)
                     /locus_tag="LGJ20_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2387396.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            10080..11081
                     /locus_tag="LGJ20_003377"
     CDS             10080..11081
                     /locus_tag="LGJ20_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK2387397.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            11661..12778
                     /locus_tag="LGJ20_003378"
                     /pseudo
     CDS             11661..12778
                     /locus_tag="LGJ20_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13132..13698
                     /gene="slp"
                     /locus_tag="LGJ20_003379"
     CDS             13132..13698
                     /gene="slp"
                     /locus_tag="LGJ20_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBK2387398.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            13851..14381
                     /locus_tag="LGJ20_003380"
     CDS             13851..14381
                     /locus_tag="LGJ20_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2387399.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(14423..15070)
                     /locus_tag="LGJ20_003381"
     CDS             complement(14423..15070)
                     /locus_tag="LGJ20_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK2387400.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(15134..15460)
                     /gene="hdeB"
                     /locus_tag="LGJ20_003382"
     CDS             complement(15134..15460)
                     /gene="hdeB"
                     /locus_tag="LGJ20_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBK2387401.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(15576..15908)
                     /gene="hdeA"
                     /locus_tag="LGJ20_003383"
     CDS             complement(15576..15908)
                     /gene="hdeA"
                     /locus_tag="LGJ20_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBK2387402.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            16163..16735
                     /gene="hdeD"
                     /locus_tag="LGJ20_003384"
     CDS             16163..16735
                     /gene="hdeD"
                     /locus_tag="LGJ20_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBK2387403.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            17534..18061
                     /gene="gadE"
                     /locus_tag="LGJ20_003385"
     CDS             17534..18061
                     /gene="gadE"
                     /locus_tag="LGJ20_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBK2387404.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(18062..18340)
                     /locus_tag="LGJ20_003386"
     CDS             complement(18062..18340)
                     /locus_tag="LGJ20_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387405.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            18400..19557
                     /gene="mdtE"
                     /locus_tag="LGJ20_003387"
     CDS             18400..19557
                     /gene="mdtE"
                     /locus_tag="LGJ20_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBK2387406.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            19582..>21227
                     /gene="mdtF"
                     /locus_tag="LGJ20_003388"
     CDS             19582..>21227
                     /gene="mdtF"
                     /locus_tag="LGJ20_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HBK2387407.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAG"
BASE COUNT         5485 a         5109 c         4966 g         5667 t
ORIGIN      
        1 ggagcacttg tgctggcgat tggctacttc atgaccggca tgtcgctact taagcctgac
       61 ctgattttca tcgccctggg gactatcgct gtcggtaacg gcctgtttaa agctaaccca
      121 gccagcttgc tttcgaagtg ctatccgccg aaagatccgc ggcttgatgg cgcattcacc
      181 ctgttctata tgtcgatcaa tatcggctcg ttgatagcgt tatcgctggc ccctgtgatc
      241 gctgatagat tcggttattc agtcacctac aacctgtgcg gggcggggtt aattatcgca
      301 ttactggttt acatcgcctg tcgtggaatg gtgaaagaca ttggttctga acccgacttc
      361 aagccgatga gcttcagcaa actgttgtac gtattacttg gcagcgtggt gatgatcttc
      421 gtatgtgcat ggctgatgca caacgtagaa gtcgccaatc tggtgctgat tgttctctcc
      481 atcgtcgtca ccatcatctt ctttcgtcag gcattcaagc tggataaaac cgggcgcaat
      541 aaaatgtttg tcgcctttgt cctgatgctc gaagcggtgg tgttttacat tctctacgcc
      601 cagatgccaa catcgctgaa cttctttgcc atcaacaacg tgcatcatga aattctcggt
      661 ttttccatca acccggtcag cttccaggcg cttaacccgt tctgggtggt actcgccagc
      721 ccaatactgg caggcattta cacgcatctg ggtaacaaag gcaaagacct ctcgatgccg
      781 atgaaattta ctctcggcat gtttatgtgc tctctgggct ttttgacggc tgcagctgcg
      841 ggaatgtggt ttgcggatgc ccaggggctg acatcgccat ggtttatcgt gctggtgtac
      901 ttattccaga gcttaggtga actgtttatt agcgcccttg gcctggcgat gattgctgcc
      961 ctggtgccgc agcatttgat gggctttatt ctcgggatgt ggttcctgac gcaggctgcc
     1021 gcgttcttgc tgggcggcta tgtggcaaca tttaccgcgg tgccggacaa cattaccgat
     1081 ccgcttgaga cgttgcccgt ctataccaac gtgtttggta agattggtct ggtcacactg
     1141 ggcgttgcag tagtgatgct gttgatggtg ccgtggctga aacgcatgat tgcgacgccg
     1201 gaaagccatt aattattctt gcagaaagca gggtagcgtt atcgctaccc tggtttactt
     1261 ttacactggc gtacctgcat aaatatcaaa cctatgccct ttagtgacta ccgcgtttgg
     1321 cgtggcgaca ttcgccagcg gtggcgcgta gtccgggcgc tttaccacca cgcgtttggt
     1381 cgccagcaag cgtgcaggct ccagtaatcc atcggcatca agatccggtc ccaccagcga
     1441 ctgaaagaca cgcatctctt ttttcactag cgcgcttttc tgcttatgcg ggaacatcgg
     1501 gtcgagataa accacctgcg ggcgcggggt aatatcagtc agcgccgtca ggctggaggc
     1561 gtgaattaac tgcaaccgct cctgcaacca gccgccgatt tccgcatccg cataaccacg
     1621 cgccaggccg tcgtcgagca atgcggcaac gactggatta cgctccagca tccgcacgcg
     1681 gcagccgact gaagccagca caaaggcatc gcgccccagc cctgcggtgg catccaccac
     1741 atccggcaaa taatcgcctt taatgcccac cgctttcgcc accgcctcac cgcgaccacc
     1801 gccgaatttg cgtcggtgcg ccatcgctcc gccaacaaaa tcaacaaaga tgccgccaag
     1861 ttttggctca tcacgcttgc gcaattccag atgttccggc gttaacacca gcgccatcag
     1921 gttgtcttca tcgtgctcca gcccccagcg gaccgccaga acagataagg caccgtctcc
     1981 ggtgcctgtt tcatcaatta agcagatttt cactgaatga tcagccctta atgccgtaat
     2041 gctccagcat cgcatccagc tgcggttcac gaccacggaa gcgtttgaac agatccatcg
     2101 gctcttctga accgccacgg ctcagaatgt tgtcgaggaa cgactgcccg gtttcacggt
     2161 tgaaaatgcc ctcttcctca aagcgcgaga aagcatctgc cgccagtacg tcagcccaca
     2221 ggtagctgta gtaacctgcg gcataaccac cggcgaaaat atggctgaaa gcgtgcggga
     2281 aacggcccca ggatggagac ggtactacgg caaccagttt cttgatttct gccagagttt
     2341 cgaggatttt cgctccctga tccgggcgga actcggcatg gaggcggaaa tcgaacaggc
     2401 cgaactccag ctggcgcaga ataaacagcg ccgcctggta gttcttcgcc gccagcattt
     2461 tatccagcaa ctctttcggc agcggttcgc cggtttcgta gtgaccggag ataaacgcca
     2521 gcgcctccgg ctcccagcac cagttttcca taaactgact cggcagttcg accgcatccc
     2581 acggcacacc gctgataccg gaaacaccag cggtttcgat gcgggtcagc atatggtgca
     2641 ggccgtgacc gaactcgtgg aacagggtga tcacttcgtc gtgagtgaac agcgccggtt
     2701 taccatttac cgggcggttg aagttacaag tcaaatacgc gaccggtttt tgcagcgaac
     2761 catcagcttt acgcatctgg cctacgcagt catccatcca cgccccgccg cgcttgtttt
     2821 cacgggcata cagatcgagg tagaagctgc cgcgcagttc gttattttcg tcatacagtt
     2881 cgaagaaacg tacatccgga tgccagacat caacatcttt acgctcttta gcggtgatgc
     2941 cgtaaatacg tttcaccact tcaaacaggc cgttaaccgc tttgttttct gggaagtacg
     3001 gacgcagctg ctcgtcgctg atgctgtaga ggtgctgttt ttgtttttcg ctgtagtacg
     3061 cgatatccca cggctgcaac tcatcgacgc caaattccgc tttggcgaag gcgcgcagtt
     3121 gtgccagctc tttttcgcct tgcggacgcg cgcgttttgc cagatcggtt aagaaatcca
     3181 gcacctgctg cgggttttct gccattttag tggcaaggga tttaaaggcg tagttttcaa
     3241 agcccagcag ttgcgccagt tcgtgacgca gagcgaggat ctcttccatc accttgctgt
     3301 tatcccattt accggcgttc gggccttgat cggaggcgcg ggtgctgtaa gcgcgataca
     3361 tctcttcacg caaggcctgg ttgtcgcagt aggtcattac cggcaggtag cttgggatat
     3421 ccagcgtcag caggtagcct tccagctctt tcgcttcggc ctgggctttt gccgcagcca
     3481 gcgcgctttc aggcatcccc gccagctccg cttcgtcggt aacgagtttg gtccagccca
     3541 tcgtcgcatc gaggacgttg ttgctgtact ggttgcccag ctcggaaaga cgagtggcaa
     3601 tctcaccgta gcgttgttgt ttctctattg gcagaccgat gccagagagt tcgaagtcgc
     3661 gcagtgcgtt atcaaccgct tttttctgag ccgtgttcag cgtggcgtaa tgatcgccat
     3721 cgcgcaggtc gcgatacgct ttatacagcc cttcatgttg ccctacccag gtgctgtatt
     3781 ccgacagcag cggcagggtt tgttcgtacg cttcacgcag ttccgggcta tttttcaccg
     3841 agttcaggtg gctgaccggg gagaagatac gccccagcac atcgtccact tccgccaacg
     3901 gctggcagag attttcccag gtgtacggtg ctccttgcgc tactacgcgc tccacatttt
     3961 cgcggcagtc gttcagcgcc ttagtcacgg ctggaacgac atgttccggg agaattttag
     4021 aaaacggagg caattcaaag ggagtcagta acggattcgt cattcgcgca gtcctggtta
     4081 aagaggttaa ggaagcgctc aacaggcgct ttacataatg tgtgtagcat ggggttaagt
     4141 gtagtgaatt tcaatgagaa acgttacgct ttcgcggcgg cggcctcttt tcggtatact
     4201 gtcctgatac gcttttgtgc gccccgaata cgggccgatt tttacttacc ggaacacctt
     4261 tacccatgct cagttatcgc cacagctttc acgctggcaa ccacgccgac gtccttaaac
     4321 ataccgttca gagcctgatc atcgagtcgc tgaaagagaa agataaaccg tttctctatc
     4381 tcgacaccca cgcaggggcc gggcgttatc agttaggcag cgaacatgcc gagcgtaccg
     4441 gcgaatatct cgaaggcatc gcccgtatct ggcagcagga cgatttgccc gcagaactgg
     4501 aggcgtacat caatgtggta aaacacttca accgtagcgg tcagttgcgt tactaccccg
     4561 gttcgccatt gattgctcgc cagctactgc gcgaacaaga cagcctgcaa ctgaccgaac
     4621 tgcacccgag cgattacccg ttgctgcgtt ctgaatttca gaaagacagc cgtgcgcgcg
     4681 tcgaaaaagc cgatggtttc cagcagctta aggccaaact gccgccggtt tcccgccgtg
     4741 gtttaatcct tatcgacccg ccgtatgaaa tgaaaaccga ctatcaggcg gtggtcagcg
     4801 ggatagcaga aggttacaaa cgtttcgcca ctggtactta cgcactgtgg tatccggtgg
     4861 tgctgcgtca gcaaattaag cgcatgatcc acgatctgga agcgaccggt attcgcaaaa
     4921 ttctgcaaat tgaactggcg gtactgccag acagcgatcg ccgtggcatg accgcttccg
     4981 gcatgattgt gattaacccg ccgtggaaac tggaacagca gatgaataac gtgctgccgt
     5041 ggctgcacag caaactggtt ccggcaggca ccgggcacgc caccgtaagc tggatcgtgc
     5101 cggagtaatt gcagccattg ctggcaccta ttacgtctcg cgctacaatc gcggtaatca
     5161 acgataagga cactttgtca tgactaaaca ctatgattac atcgccatcg gcggcggcag
     5221 cggcggtatc gcctccatca accgcgcggc tatgtacggc cagaaatgtg cgctgattga
     5281 agccaaagag ctgggcggca cctgcgtaaa tgttggctgt gtgccgaaaa aagtgatgtg
     5341 gcacgcggcg caaatccgtg aagcgatcca tatgtacggc ccggattatg gttttgatac
     5401 cactatcaat aaattcaact gggaaacgtt gatcgccagc cgtaccgcct atatcgaccg
     5461 tattcatact tcctatgaaa acgtgctcgg taaaaataac gttgatgtaa tcaaaggctt
     5521 tgcccgcttc gttgatgcca aaacgctgga ggtaaacggc gaaaccatca cggccgatca
     5581 tattctgatc gccacaggcg gtcgtccgag ccacccggat attccgggcg tggaatacgg
     5641 tattgattct gatggcttct tcgcccttcc tgctttgcca gagcgcgtgg cggttgttgg
     5701 cgcgggttac atcgccgttg agctggcggg cgtgattaac ggcctcggcg cgaaaacgca
     5761 tctgtttgtg cgtaaacatg cgccgctgcg cagcttcgac ccgatgattt ccgaaacgct
     5821 ggtcgaagtg atgaacgccg aaggcccgca gctgcacacc aacgccatcc cgaaagcggt
     5881 agtgaaaaat gccgatggta gcctgacgct ggagctggaa gatggtcgca gtgaaacggt
     5941 ggattgcctg atttgggcga ttggtcgcga gcctgccaat gacaacatca acctggaagc
     6001 cgctggcgtt aaaactaacg aaaaaggcta tatcgtcgtc gataaatatc aaaacaccaa
     6061 tattgaaggt atttacgcgg tgggcgataa cacgggtgca gtggagctga caccggtggc
     6121 agttgcagcg ggtcgccgtc tctctgaacg cctgtttaat aacaagccgg atgagcatct
     6181 ggattacagc aacattccga ccgtggtctt cagccatccg ccaattggta ctgttggttt
     6241 aacggaaccg caggcgcgcg agcagtatgg cgacgatcag gtgaaagtgt ataaatcctc
     6301 tttcaccgcg atgtataccg ccgtcaccac tcaccgccag ccgtgccgca tgaagctggt
     6361 gtgcgttgga tcggaagaga agattgtcgg tattcacggc attggctttg gtatggacga
     6421 aatgttgcag ggcttcgcgg tggcgctgaa gatgggtgca accaaaaaag acttcgacaa
     6481 taccgtcgcc attcacccaa cggcggcaga agagttcgtg acaatgcgtt aaatgttaaa
     6541 gggctaagag tagtgtgctc ttagccctta attacgtttc cgctatcagt tcagaagctg
     6601 aagcagaaag cggatcagtt ccagcagcgc aattaacgcc ccaagaacga tgattgcttt
     6661 atcaatcacc cgttttctcc atgcgatgga gtgagaatgc atccgcttac tcatccactg
     6721 cctgtcacgg cgcatgccct attgttagat aatggcattc tcactccggc cagagcatca
     6781 gttaacggca ccacccgtac ttctgaccag gactttgaaa gcgtttatgc gcattaccag
     6841 agtgaaaatg cctcagagct aactggataa tcatacagta cattcaggtt ataaaaccag
     6901 catgtccttg caatagtttc agtatggtat tagcattgat gcgttagata atggcattct
     6961 cactccagcc agtgccacca actcagggct ggaaagtaaa aaaccgacgc aaagtcggtt
     7021 tttttacatc cggattcgga caaggcttaa tatgacgatg acccagtgaa agtatataaa
     7081 tcgtcattgc gatatatacc gaagtgcccc ctccgccagc tgaagaaatc gctaattctt
     7141 gcaatgttag ccactggcta atagtattga gctgttagat aagaactctc tcactccagc
     7201 cagagccacc aactcagggc tggaaagtaa aaaaccgacg caaagtcggt ttttttacgt
     7261 cctgattcag acctcctttc aaatgaatag ccaactcaaa attcacacct attaccttcc
     7321 tctgcactta cacattcgtt aagtcatata tgtttttgac ttatccgctt cgaagagaga
     7381 cactacctgc aacaatcagg agcgcaatat gtcatttctg ttacccatcc aattgttcaa
     7441 aattcttgct gatgaaaccc gtctgggcat cgttttactg ctcagcgaac tgggagagtt
     7501 atgcgtctgc gatctctgca ctgctctcga ccagtcgcag cccaagatct cccgccacct
     7561 ggcattgctg cgtgaaagcg ggctgttgct ggaccgcaag caaggtaagt gggttcatta
     7621 ccgcttatca ccgcatattc cagcatgggc ggcgaaaatt attgatgagg cctggcgatg
     7681 tgaacaggaa aaggttcagg cgattgtccg caacctggct cgacaaaact gttccgggga
     7741 cagtaagaac atttgcagtt aaaaatttag ctaaacacat atgaattttc agatgtgttt
     7801 tatccgggag gcattatgtt actggcaggc gctatctttg tcctgaccat cgtattggtt
     7861 atctggcagc cgaaaggttt aggcatcggc tggagtgcaa cgctcggcgc agtactggcg
     7921 ttagttacgg gcgtggtcca tccgggtgat attccggtgg tgtggaatat cgtctggaac
     7981 gcgacggctg cgtttatcgc cgtcattatc atcagcctgc tgctggatga gtccggcttt
     8041 tttgaatggg cggcgctgca cgtctcacgc tggggtaatg gtcgtggtcg cttgctgttt
     8101 acctggattg tcctgctcgg tgctgccgtt gccgccctgt ttgccaatga tggcgcggcg
     8161 cttattttga caccgattgt catcgccatg ctgctggctt tagggttcag taaaggcact
     8221 acgctggcgt tcgtgatggc ggccggattc attgccgata ccgccagcct gccgcttatt
     8281 gtctccaacc tggtgaatat cgtttccgct gatttctttg gcctcggctt tcgcgaatac
     8341 gcctcggtga tggtgccggt ggatatcgcc gcgattgttg ccacgctggt gatgttacat
     8401 ctctattttc gcaaagatat tccgcagaac tacgatatgg cgctgctgaa atctcccgca
     8461 gaagcgatca aagatcctgc tacgttcaaa actggctggg ttgttttact gcttctgctg
     8521 gtgggatttt tcgtcctgga accgctcggc attccggtga gcgccattgc agctgtgggc
     8581 gcgctgatat tatttgtcgt cgctaaacgc ggtcatgcga ttaatacggg taaagtcctg
     8641 cgcggtgccc cctggcagat tgtcatcttc tcgctcggca tgtatctggt ggtttatggc
     8701 ctgcgcaatg ccggattaac ggaatatctt tctggcgtac tcaacgtgct ggcggataac
     8761 ggcctgtggg ccgcgacgct cggcaccgga ttcctcaccg ccttcctctc ttctattatg
     8821 aacaatatgc cgacggtact ggttggcgcg ttgtccattg atggcagcac ggcatctggc
     8881 gttatcaaag aagcgatggt ttatgccaat gtgattggct gcgatttggg accgaaaatt
     8941 accccaattg gtagcctggc tacgctactc tggctgcacg tactttcgca gaagaatatg
     9001 actatcagct ggggatatta cttccgtaca gggattatca tgaccctgcc ggtgcttttt
     9061 gtgacgctgg ctgcgctggc gctacgtctc tctttcactt tgtaatgaga tattgatatg
     9121 agcaacatta ccatttatca caacccggcc tgcggtacgt cgcgtaatac gctggagatg
     9181 atccgcaaca gcggtacaga accgaccatt atctattatc tggaaacacc gccaacgcgc
     9241 gatgagctgg tcaaactcat tgccgatatg gggatttccg tacgcgcgct gttgcgtaaa
     9301 aacgtcgagc cgtatgagga actgggcctt gcagaagata aatttactga cgatcggtta
     9361 atcgacttta tgcttcagca cccgattctg attaatcgcc cgattgtggt gacgccgctg
     9421 ggaactcgcc tgtgccgccc ttcagaagtg gtgctggaaa ttctgccaga tgcgcaaaaa
     9481 ggcgcattct ccaaggaaga tggcgagaaa gtggttgatg aagcgggtaa gcggttgaaa
     9541 taaagcggcg atatccactg cccatagttg gtaaagtgcg ctttgtttat gtcggatgcg
     9601 acgctaacgc gtcttatccg acctacaaaa ccgtgcaaat tcaatatatg gcatgaaata
     9661 tgtagagcgt agcgcatcag gcaattttgc tatttacgca gcactcctcg cgtaagaatt
     9721 caattatcgc ttccagcatt gcgtattgcg aaacacacat cagcgtccga ctctcacggt
     9781 tttgtttgac cagtcctacc gataccagtg ctgatatatg gtggctgagt gtggagccag
     9841 gtatcccaag ctgcttttgc aactcaccga ccggcaaccc ttgttccccg gctttgacaa
     9901 gatgtttaaa tatgaacaaa cgggtcgggt gacctagctc tttcagcgct ttagcgactt
     9961 cttccaactg catatgacac ccctctgtct ggttgatatt tcgataatac cagaaatatc
    10021 ttgatctgaa tttcatttcg attattctgg aaatatagat ttatccaatg aggttacgta
    10081 tgagtagttg gcttgcgatg ctgcaagatg ccgcagagat gtttgtgttt ctcgccgtcg
    10141 agctttcgtt gctgtttata gtgattagtg ccggtgtcag cctgataaga caaaaggtgc
    10201 cagaccataa aatccagcag atgatggggg ccagaaaagg gagaggctat ctcctggctg
    10261 ctctgttggg agccgttacc ccgttctgta gttgctcgac aatacccatg ttacgtggat
    10321 tgttgtcagc gaaagccggg tttggtccga ctctcacttt tttatttgtt tcaccattac
    10381 ttaatcccat tatcgtcgga ctaatgtgga tgacctttgg ttggaaagtc accttgttgt
    10441 acgcgattat cgcagccggc gtctccgttc ttgccagtat tatcctggat tctctggggt
    10501 ttgaacgtca tatcattgcc agtaaaagct catcagcaaa ttgttgtgct ccagcaaaaa
    10561 cttcgccggg aacgacatat acgcccataa aagtgagttg ttgtagtcca gcggctaaag
    10621 ccattgagaa ccccgtagtt acctgttgca gtaccaaagc tgtagtaagt attaatccca
    10681 taaaactagc caccaaagat gcgttgcaac agtttaaaga tgtactgcca tatcttttgt
    10741 taggagtatt aataggatct tttatttatg gctttattcc ctcagcgtgg attgccgctc
    10801 atgcaggagc agataatccc ttcgccatcc cattaagcgc cgttgtcggt attccgctgt
    10861 atatccgggc tgaggcagtt atccctctgg catctgttct gatgacaaaa ggaatgggtc
    10921 tgggagcatt aatggcatta atcatcggca gtgccggcgc aagcctgacg gaagtgatat
    10981 tacttaaatc aatgttcaga atgccgatga tagctgcatt cctgacggtt atattaggta
    11041 tggctatctt gatgggctat ttgactcaat tgctatttta aatagatgcc agcagtaaac
    11101 tataagatgc atagttagtg ccatatcctt ctaagtagaa aatcatagac aggataatcc
    11161 ttaaaaatca caaatgattt aactaatgca ttgagaatga tgacggctcg gtcaacaagt
    11221 catcaacaga caataaccac gtaatcatgc ttctctgaca ttttatcgga gaagcaatga
    11281 ctctttgtct cagtaccctc ctccttttta aaaggcagat ttcagtcgct aatacaacac
    11341 atttttaaat acactttaac ctggttcatt attacacatc taaaacatca caaaaatatt
    11401 acttcacaca ctatcggaga acaaaataac tttttaaaat gagcgctttt tgatagttgt
    11461 taacgttttc ctttacgata ctatcactgc ctttttttat acacaaatat taccgggtgg
    11521 aaatgaaagg gaatttaaag gtaattatat caggaagata taacgcatta tatttattgt
    11581 gtgtagaaga aaatccattc ctgttctaat tatccaattt aaaaacctta gcataaaaca
    11641 gatgatgaat aaggaatgtt atgtcaattg actttacccc aggtataata aatacatatc
    11701 acggcgatat ttataactgc acaacaaata ccgataatgt taaaacgcca gacacatcga
    11761 agtggccttg tgataattgg gaagaacaac aacccattaa ttccacattc tctggagaag
    11821 gatacaaccc tgaacaattt gatttagcgc aacaacaact ccaacaaatt aatgcatgcc
    11881 acacgaacac cacatataca aatgcagact actcaaaagt tgtggttcaa cttgtaagtc
    11941 ttattaataa cattgaaacg ataagttcga caccacttac gcaacagaca caatcaatac
    12001 ttaatcagat tcataacatt agatatgaaa aaaaaacaaa agtgctgaat gtcgaatcat
    12061 tgttgtcgct aatcctaaac ccgacaaggc aatcataact aaaattagcg tagaagaggg
    12121 aatacccata acattcagtg tccaaaccat gttttcagat actaatttta ttgctgaaca
    12181 acgagctgac ttaccaacta atataaaaga tattcaaagc ctgtatcaaa aaatgacgaa
    12241 gttatatatt gagcacagtg aaaataaaaa caggatgaaa gtctttgccg gaaccaactt
    12301 tattgatttc aatatgacag gacagaacct gtccgggttc gttctaacct tgtcaaggtt
    12361 ttatttcgaa gatctactta atataaattt cactgacgca aatttaggtg acactatttt
    12421 ttacataaag aacaccctac ccccaaatta tataaagatg gacaatatct tgacaaacaa
    12481 atcgaaggtt tattttcaac attattaacc attaatgaca actttttacg agcaaaagcg
    12541 gaaattgctt cgacaataat taaattttta gaggctagga taaccaatct ttcatacaat
    12601 gacatattaa catatcagca agaattccaa aaacaatgtt ataagcaagt taaagccttt
    12661 gcgacactct ctcgatacaa taaaattcaa acatgggcag aaatgtctga atatcagttc
    12721 gaagtatttc aatacgagac gcttaacccc aaaaaaatgt cacacacgcc ttatttaaag
    12781 aggccacttc ctaatgaaaa agatataaat tatggggtgg aaatagaaat accatctggc
    12841 aaaagaattc gcttatcaaa tcattatcag aatataatac cataatattg attattagca
    12901 ctttataatt attgaataaa aatagatttt atgtaatttt aaaacaaagg gttatattat
    12961 tgggcgatgg atactcatac cacgccccaa aataacggaa ttgaactaaa cagaagggat
    13021 ataaacatca gacaggttta cgttactatc aggcatatca cctcagaatc agatgaaaac
    13081 tataaagaaa tatctattat ggttttaata tttgttgata aggatagtaa catgaacatg
    13141 acaaaaggtg cactcatcct cagcctttca tttttgcttg ccgcatgtag ttcaattccg
    13201 caaaatatca aaggcaataa ccaacctgat attcaaaaaa gttttgttgc tgttcataac
    13261 cagccggggt tatatgttgg tcaacaagcg cgctttggtg ggaaggttat caacgttatc
    13321 aatggcaaaa cggatacgtt gttagaaatc gctgtattac cgttggatag ctatgcgaag
    13381 cctgatattg aagccaacta tcagggccga ctgctcgcca gacaaagcgg cttccttgat
    13441 ccagtgaact atcgtaatca ctttgttacc atcctcggta ccattcaggg tgaacaacca
    13501 ggctttatca ataaagtccc gtataacttc ctggaagtga atatgcaggg catccaggtg
    13561 tggcatttga gagaagtggt taataccacc tataacctgt gggattacgg ctatggtgca
    13621 ttctggccgg aaccgggctg gggtgcgcct tactacacca atgcggtgag tcaggtaaca
    13681 cctgagctgg tcaaataaca ccaccgaaag atgcagatgc tttctcagca tctgcatcat
    13741 gtattacata aaattaatat acagtaagct aactattatt ataagccctg tcctgttaat
    13801 tacctttggc aaactgatta taaagttaat gtccgcacca ggagtcggtt atgtttctta
    13861 taattaccag ggatacgatg ttcttcaccg cgatgaaaaa cattctgagt aaaggtaatg
    13921 tcgttcatat acagaacgaa gaagagatcg acgtaatgtt gcatcagaat gccttcgtca
    13981 ttattgatac attaatgaat aatgtatttc attctaattt tctcactcaa attgaacgat
    14041 taaaacctgt ccatgtgatt attttctccc cctttaatat taaacgctgt ctggggaaag
    14101 taccggtgac ctttgttccg cggactatca ctatcattga ttttgtcgca ctcatcaatg
    14161 gcagttactg ctctgtgcct gaagcgaatg tgtcactttc gcgcaagcaa catcaggttc
    14221 tgagctgcat tgcgaatcaa atgacaacgg aagatattct ggagaaactg aaaatatcgc
    14281 taaaaacgtt ctactgccat aaacacaata tcatgatgat cctcaatctt aagcggatca
    14341 atgagctggt acgccatcag catattgatt atctggtgtg aatttcaggc ttacggtgag
    14401 tctggctacg ctgccacaca gattagctaa ttgaaacgcc tttcacccct gccatacctt
    14461 ttaataatcg caacaggtct tctatcgacg ttgtggcatg aagctggata tcaatggcga
    14521 ccacttcatg atcttcattt tcctgcaacg aaactaaatc cgtttttatt ttttgctgct
    14581 taaaccagtc gagcattgat accgtattac cgttcactaa tgtcagctgt aaatggtaat
    14641 ttttgttcat caggcggaag gttaattgat ggaagacttc cagcaccaac aaggtcatca
    14701 ctgaaccata aatacccagt tcgtacatac cgctgccaat aaccatacct atggcggcgg
    14761 tcacccagat atccgctgcc gtcgtcagac ctacaatatt ttggttacga acgagaatgt
    14821 tacctgcacc gataaacccg acgcccgtca ccacctgagc agcaatacgg ctggggtcga
    14881 gtccgacgtg atctaaagac agcacgtcag caaaaccata tttcgaaaca atcataaaca
    14941 gggcgcttcc catgccaatt aatacatgtg tgcgtaaccc tgctcctttg ccgcgcattt
    15001 gcctttccat gccaatagcg ccacaggcaa ttgccgcgag tataagacgt ataataaatt
    15061 ctgctgtcat ataaaaatgc aaaaaggagc agcaagatgg ctcaacttgc tactcctttt
    15121 tacttgtacc tcattaattc ggcaagtcat tagatgcttg atttttgaag gtatacaaat
    15181 ttttctgcgg gtttttctta cagtattcga tcactttagg aatttgagtg agatcggttt
    15241 catttaaagt aacggtatcg ccacctttat atactgtttc ttcatgcagc atccaccatg
    15301 caaccggggt cattgctttt ggattcagat caataaattc ctggcaggtc atatctttag
    15361 cggattcatt ggctgccagc gcagattgtg cgttcaccag tgacaaagcc gctacagcgc
    15421 ccataaaaat aaacgcttta cggagagatg aaatattcat tttgtaaccc attcaatata
    15481 gagattatat atgaacagga ggaatagctg cttttaaaga agatattccg gcctgttcaa
    15541 tatgcaagga agtacgatgt aacgcattcc cggaattaca tatctttctt aattttgtcc
    15601 cattcgcctt taactttatc tttaaagttg gcttgtttat cctgagtaca agcctgaacg
    15661 atagctgggg ttacggttgc aataccctga acatctaaaa ccgcatcttc tggtttatct
    15721 ttgttgttca gcgcttcagc aaaaccaact gcagttggct ggaaggattc gtccacagcc
    15781 aggaaatctt cacaggtcca ggagttgacc ggttttttgt tatcagctgc tttttgcgca
    15841 tccgctgcat tgcttacaac tggcagaaga agcagaccac caagaataac gcctaatact
    15901 tttttcatcg taatatcctc aactataaag tgaaagagcc gtcacgaatc aatttcgaca
    15961 ctgaggttat aacctggttt tctgtatatg tcatgttgat agaaaatatc aaaatcagat
    16021 atttttattt caatacaatg agttacagat gcatcagata ctgtaattag gaaattttta
    16081 ttaaatcgac tgcattctta gacgcgtttt tggcatagat tgatagcagg ggattttctt
    16141 cttaatttta tagggtggtt ctatgttata tatagataag gcaacaattt tgaagtttga
    16201 tctggagatg cttaaaaaac atcgcagagc aatccagttt attgccgtgc tgttgtttat
    16261 cgtcgggttg ctgtgtatca gtttcccgtt cgtctctggc gatattttaa gcacagtagt
    16321 gggtgcatta ttaatctgct cgggcattgc gcttattgtc gggttattca gcaaccgcag
    16381 tcataatttc tggccggtat tatccggttt cctcgtcgca gtcgcctatt tattgatcgg
    16441 ctatttcttc atccgcgcac cggagctggg catttttgcc attgcggcat ttattgccgg
    16501 tttgttctgt gttgcagggg ttattcgcct gatgagttgg tatcgtcagc gttcaatgaa
    16561 aggcagctgg ctacagctcg ttattggtgt gctggatatc gtcattgcct ggatattcct
    16621 tggtgcaacg ccgatggtgt ctgtaacgct ggtttccaca ctggtgggaa ttgaactgat
    16681 atttagcgcc gccagcctgt tcagcttcgc cagtttgttc gttaagcagc aataattatc
    16741 ccggttgtca cccggatcat agtcacttga tgtgactatg atccgattaa tactctctcc
    16801 gctacgcagc gttgtagatc aattgcgcac tatcattgaa ataattacct gctagtgatt
    16861 atttcaacct actgaatttc atctaatttt tttcactcca tggcaaatta gccatttcaa
    16921 acattatcat ggctgatatt ttccgtagtc aggtttaatg ttttaaaagt gcagtgggaa
    16981 agtgaacaaa gagttccgta agcgttgatg ctatgggcgg ttaaataagt aatccgggtt
    17041 catttttttg caactggcat tgattacatt gcataaatat ccgtgtctcc agacgctata
    17101 taaaaacctg aagacatgaa tgcgttattt actcaggtaa tttcaatgcg ttaaaagaaa
    17161 gctggcaatc caattgccag cttaagtcga aacaaggaga ctcgatattt aaatcggatt
    17221 acattttaac tttagtaata ttcttcagag atcacaaact ggttattgat aacttattct
    17281 tgggcagtaa tccgcaaacg ttaacttttt gtttactgtt tacaaactga taacaaccag
    17341 gaatcttact taggatcaat atatggagtg cgtgatggat aaatctgaag tattgattag
    17401 tgttaataga cgtattagtt cacgaagggc aaagttctta taggcgttta ctatattgaa
    17461 caacgattcg gacaaggatg taaataatga aaaggatgac atgttcgaaa cgataacggc
    17521 taaggagcaa gttatgattt ttctcatgac gaaagattct tttcttttac agggcttttg
    17581 gcagttgaaa gataatcacg aaatgataaa aatcaattcc ctgtcagaga tcaaaaaagt
    17641 aggcaataaa cccttcaagg ttatcattga tacctatcac aatcatatcc ttgatgaaga
    17701 agcgattaaa tttctggaga aattagatgc cgagagaatt attgttttgg caccttatca
    17761 catcagtaaa ctaaaagcta aagcgcctat ttattttgtt agccgcaaag aaagtatcaa
    17821 aaatcttctt gagattactt atggtaaaca cttgccccat aagaattcac aattatgttt
    17881 ttcacataat cagttcaaaa ttatgcaact gattctgaaa aataaaaatg aaagcaatat
    17941 cacgtcgacg ctcaatattt cgcaacaaac attaaagatt cagaaattca acattatgta
    18001 caagctgaaa ctaagacgta tgagcgacat cgtcaccctg ggtatcacat cttattttta
    18061 gtcaggacat aagcaactga aattgatggc tggcatgacg agggatgcag atgctgattt
    18121 cattaccccc ggtgattact aaaggagagg ctaaaacgac tttattcccc tggtatgtgt
    18181 atccaccagt agaacccttc gttgcccgaa tgctggcagg aactgttggc agaacggcaa
    18241 catttttttt gtcgttgacc tcaccatgtc gatcactata cctgtatccc accttactgg
    18301 ctgacaaccc cactatgccg ctggtctgta aatccctcat atctctcctc gcgcgcaatt
    18361 taaagaaccg ttatttctca agaattttca gggactaaaa tgaacagaag aagaaagctg
    18421 ttaataccgt tgttattctg cggcgcgatg ctcaccgcct gcgatgacaa atcggcggaa
    18481 aacgccgccg ccatgacgcc tgaggtcggt gtcgtcacac tctcccccgg ttcggtcaat
    18541 gtgttgagcg aattgcccgg tagaaccgtt ccttatgaag ttgccgagat acgtccccag
    18601 gtgggcggta ttatcattaa acgcaacttt atcgaaggcg ataaagtgaa ccagggcgat
    18661 tcgctgtatc agattgatcc tgcaccttta caggccgagc taaactccgc caaaggctcg
    18721 ctggcgaaag cgctctctac cgccagcaat gcccgcatca cctttaaccg ccaggcatcg
    18781 ttgctgaaga ccaactacgt tagccgtcag gattacgaca ccgcgcgcac ccagttgaat
    18841 gaagcagaag ccaatgtcac cgtcgccaaa gcggctgttg aacaggcgac gatcaatctg
    18901 caatacgcga atgtcacctc gccgattacg ggcgtcagcg ggaaatcgtc ggtgaccgtc
    18961 ggcgcactcg ttaccgctaa tcaggcagat tcgctggtta ccgtacaacg tctggacccg
    19021 atttatgtcg atctcacgca gtcggtgcag gatttcttac gcatgaaaga agaggtcgcc
    19081 agtgggcaaa tcaaacaggt tcagggcagt acgccagtac agctcaatct ggaaaatggt
    19141 aaacgctaca gccagaccgg cacgctgaaa ttctccgacc cgacggtgga tgaaaccacg
    19201 ggctccgtga cgttacgggc gattttcccc aacccaaatg gtgacttgct gcctggcatg
    19261 tacgtcacgg cattagtgga tgaaggtagc cgccagaatg tattactggt gccgcaggaa
    19321 ggcgtcaccc acaacgccca gggtaaagca acggcgctca ttctggataa agacgatgtt
    19381 gtgcagctac gcgaaattga agccagcaaa gccatcggcg accagtgggt tgtcacctct
    19441 ggcttgcagg ctggcgatcg ggtgatcgtt tccggtttgc aacgcattcg tccgggtatc
    19501 aaagcacgag caatttcctc cagccaggaa aacgccagca ccgaatcgaa acaataacgt
    19561 tgcaggctta aggggacttt catggctaac tattttattg atcgcccggt ttttgcctgg
    19621 gtacttgcca ttattatgat gcttgcaggt ggtctggcga tcatgaactt accggttgcg
    19681 cagtatccgc agattgcgcc accgaccatt accgtcagcg ctacctatcc aggtgccgat
    19741 gcgcaaacgg tagaagactc ggtcactcag gtgattgagc aaaatatgaa tgggcttgat
    19801 ggcctgatgt acatgtcttc aaccagtgat gcggcgggta atgcctctat cactctgacc
    19861 ttcgagactg ggacatctcc tgatatcgca caggttcagg tgcaaaataa actgcaactc
    19921 gctatgcctt cattacctga agcggtgcag cagcagggga ttagcgttga taagtcgagc
    19981 agtaatattc tgatggtagc ggcgtttatt tctgataacg gcagcctcaa ccagtacgat
    20041 atcgcggact atgtagcgtc taatatcaaa gacccgctaa gccgtaccgc gggcgttggt
    20101 agcgtacagc tctttggttc cgagtacgcc atgcgtatct ggctggaccc gcaaaaactc
    20161 aataaataca atctggtacc ttccgatgtt atttcccaga ttaaggtgca aaacaaccag
    20221 atttccggtg gtcaactggg tggcatgcca caggcggcag accagcaact aaacgcctcg
    20281 atcattgtgc agacgcgtct gcaaacgccg gaagaatttg gcaaaatcct gttgaaagtt
    20341 cagcaagatg gttcgcaagt gctgctgcgt gatgtcgccc gcgtcgaact tggagcggaa
    20401 gattattcca ccgtggcgcg ctataacggc aaacctgctg ccgggatcgc catcaaactg
    20461 gctaccggag caaacgccct ggatacctcg cgggcagtca aagaggagct gaaccgctta
    20521 tcagcctatt tccccgcaag tctgaagacg gtttatcctt acgacaccac gccgtttatc
    20581 gaaatttcta ttcaggaagt ttttaaaaca ctggttgagg ctatcatcct cgtcttcctg
    20641 gtcatgtatc tgtttttgca gaatttccgt gccacaatca tcccgacgat tgccgtaccg
    20701 gtggttattc tcgggacgtt tgcgatcttg tcggcggtcg gtttcaccat caacacgtta
    20761 actatgttcg ggatggtgct ggcgataggg ttactggtgg atgacgccat cgtggtggtg
    20821 gagaacgtcg agcgtgtcat tgcggaagat aagctaccac cgaaggaagc gacgcataaa
    20881 tcgatggggc agatccaacg tgcgctggtc ggtattgctg ttgttctttc cgcagtgttt
    20941 atgccgatgg cctttatgag cggcgcaacc ggggagatct accgccagtt ctccatcacg
    21001 ctgatctcct ccatgctgct ttcagtattt gtggcaatga gcctgacccc tgccctgtgc
    21061 gccaccattc tgaaagccgc gccggaaggc ggtcacaaac ctaacgccct gttcgcacgc
    21121 ttcaacacgc tgtttgaaaa atcaactcag cactataccg atagcacccg ctcgctgttg
    21181 cgttgtaccg gtcgctacat ggtggtctac ctgctgattt gcgccgg
//
LOCUS       DAFATA010000078        21127 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFATA010000078 DAFATA010000000
VERSION     DAFATA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.079"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(60..1652)
                     /locus_tag="LGJ20_003389"
     CDS             complement(60..1652)
                     /locus_tag="LGJ20_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBK2387408.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="LGJ20_003390"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="LGJ20_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBK2387409.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /locus_tag="LGJ20_003391"
     CDS             complement(2850..3968)
                     /locus_tag="LGJ20_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBK2387410.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTAIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="LGJ20_003392"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="LGJ20_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBK2387411.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="LGJ20_003393"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="LGJ20_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBK2387412.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="LGJ20_003394"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="LGJ20_003394"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBK2387413.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="LGJ20_003395"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="LGJ20_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBK2387414.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="LGJ20_003396"
     CDS             complement(7218..7283)
                     /locus_tag="LGJ20_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387415.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="LGJ20_003397"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="LGJ20_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBK2387416.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGSALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="LGJ20_003398"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="LGJ20_003398"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBK2387417.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="LGJ20_003399"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="LGJ20_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBK2387418.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="LGJ20_003400"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="LGJ20_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBK2387419.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="LGJ20_003401"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="LGJ20_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBK2387420.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14213
                     /gene="ybiO"
                     /locus_tag="LGJ20_003402"
     CDS             11991..14213
                     /gene="ybiO"
                     /locus_tag="LGJ20_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBK2387421.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPPPGEPTL
                     "
     gene            complement(14210..15136)
                     /gene="rlmF"
                     /locus_tag="LGJ20_003403"
     CDS             complement(14210..15136)
                     /gene="rlmF"
                     /locus_tag="LGJ20_003403"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBK2387422.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15412..15672
                     /locus_tag="LGJ20_003404"
     CDS             15412..15672
                     /locus_tag="LGJ20_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2387423.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            15936..18218
                     /locus_tag="LGJ20_003405"
     CDS             15936..18218
                     /locus_tag="LGJ20_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HBK2387424.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVTTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18260..18937
                     /gene="ybiX"
                     /locus_tag="LGJ20_003406"
     CDS             18260..18937
                     /gene="ybiX"
                     /locus_tag="LGJ20_003406"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBK2387425.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19011..19277
                     /gene="ybiI"
                     /locus_tag="LGJ20_003407"
     CDS             19011..19277
                     /gene="ybiI"
                     /locus_tag="LGJ20_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBK2387426.1"
                     /translation="MASGWANDDAVNEQINSTIEDAISRARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            19542..19802
                     /locus_tag="LGJ20_003408"
     CDS             19542..19802
                     /locus_tag="LGJ20_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2387427.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(20030..21115)
                     /gene="hcxB"
                     /locus_tag="LGJ20_003409"
     CDS             complement(20030..21115)
                     /gene="hcxB"
                     /locus_tag="LGJ20_003409"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBK2387428.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         5194 a         5572 c         5193 g         5168 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaaa gcatccgccc tttttctcca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actggctcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttgccgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgtacgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt ccactcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggcagtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaagga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct atggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct attcacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgttatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ttttggtcca acccaggtgc tgcacaatat cgatttgaac
    11221 attgctcagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcggg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgtt tcggacaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccgtttaat ccacaaacct tcagcaatgc gttgacccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactggc tgattcgtct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg ccagtgggcg cggcaaaaaa atcgcgaacg cagcaactgg ctgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctactgtt actggcactg acattgtttg
    12601 tcggccaggt attaagcgac aacctgaatg cgggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggctgaa ctgcgcccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtattgc agcgtttgtt tccggtatgt tttcccgctg gctggcaaaa accatcactc
    13201 tctcgccaca tactcagcgt aactatccag agttgcaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgtatc gcacttattc tttttttctc ggcggttggc tggacggtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatattatca
    13681 ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggta actatcgggc
    13741 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    13801 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    13861 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctgatta
    13981 ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    14101 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcggccccg gctgaaccgc cgccggggga gccaacgctt taacgttggc
    14221 gattaacaaa acggcggcgc tgctcgtcgt tcataaaggt ccaggcaata aagcgactct
    14281 gcttttgccc ctgggccatc tcttttttta ccaccttcac cgcgcccacg tcggtcagag
    14341 cacgatacaa cggcggtaag ttttcaccac gagataccag cgaggtaaac cacatcacct
    14401 gcttcgcgaa gcctttgctc tcttcaatca tctttttgat aaaggcgact tcaccacctt
    14461 cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg ttcagcccca
    14521 ggttacggcg tttacgctca ctacctgccc gcgctgcagc ggcggaatcg tggaatggcg
    14581 ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat gatgccgtta aaaattgccc
    14641 cactctcttt ttgccgacgc agacgaatgg cgcggttaag ccccggatta gcactgataa
    14701 tcgcctgcgc actgcttaac gcctggctgc tggtttcact accggtaaaa cgccagccat
    14761 attcatgtac gccaatcagc ggataaatac agttcgcacc aacgccgata tccagaatgc
    14821 tggcattcgc tggaattgtt ccgcttgctt cagccagtaa atcggcaagg tgatgaatat
    14881 aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta
    14941 cggcgtaaaa atgggccagc aacgccttat tgagcgcctt caccgccagc ggattggcaa
    15001 agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga
    15061 cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg
    15121 gtttctgggc ggacatagca ttctccttta agcagcggcg taagataccc gcataacgcg
    15181 acagggtaaa tatctggctg cgtttatcat tccccaaaac cttaacatgg ctatggttta
    15241 tggcaattca tcataaaaat cagcattatg catgtcattg atttgtctta ttttgtaaaa
    15301 atatatcgtt caaaaaataa gcacatctct gtcaattttt tgcgtcattt tcatcgccca
    15361 ggcgacctac acttatcagg cactacccgt tgtatttatg aggatgtcat tatgaaaaag
    15421 tgcctcacac tactgattgc caccgtcctg agcggaatct ctctcacggc ttatgccgcg
    15481 caaccgatga gtaacctgga cagcggtcag ctacggcccg ccggtaccgt ttcggcaact
    15541 ggcgcatcaa acctaagcga tctggaggat aaactggcag aaaaagcgcg cgagcaaggc
    15601 gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc
    15661 atctacaaat aaccccacca acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt
    15721 ttgtaatcaa aaaacatcaa atatgaacgc aatgtaaata aatgtatttc tttttcgcgc
    15781 aatgggtgat agaaaatcgc gccaagtgat aatgcttatc aaaattatta tcacttgcac
    15841 gaacactatc acgagattaa cagtggcatc gcatccgcag agaggctttc tcgtggcagt
    15901 gaaaatttca acatataaaa aaagtcacct gcaaaatgga aaacaatcgc aatttccctg
    15961 ccagacaatt tcattcgctc acgttctttg ccggtctttg tattggcatc acgcctgtgg
    16021 ctcaggcact cgccgccgaa gggcaaacta acgcggatga cacgctggtt gtcgaagcat
    16081 caacgccttc gctttatgcg ccacaacaat ctgccgatcc gaaattctcg cgtccggtag
    16141 cggatactac ccgcacgatg acggtgattt ctgaacaagt gattaaagat cagggcgcaa
    16201 ccaaccttac cgacgcgctc aaaaacgtcc ccggcgtggg tgcgtttttt gcgggtgaga
    16261 acggtaactc caccactggc gacgccattt atatgcgcgg tgctgatacc tctaacagta
    16321 tttatattga tggcattcgc gatatcggca gcgtctcgcg cgacaccttc aatactgagc
    16381 aggtcgaagt gattaaaggg ccttccggca ccgactacgg gcgcagcgcg ccgacgggct
    16441 cgatcaatat gatcagcaag cagccgcgca atgattccgg cattgacgcc tccgccagta
    16501 ttggcagcgc ctggttccgc cgcggcacgc tggacgtcaa tcaggtcatt ggtgatacca
    16561 ctgcggtgcg cctgaatgtg atgggcgaaa aaacgcacga tgccggacgc gacaaagtca
    16621 aaaatgagcg ttacggcgtc gccccttcta tcgcttttgg ccttggtaca gcgaatcgtt
    16681 tgtatcttaa ttatctgcat gtcacccagc acaacacgcc agacggcggc attccgacca
    16741 tcggtttgcc gggttattct gccccatctg cgggaacagc ggctctgaat cattccggaa
    16801 aagttgatac tcataacttt tacggcacgg attccgatta cgacgattcg accaccgaca
    16861 ccgccaccat gcgttttgag cacgacatca acgataacac caccattcgc aatactaccc
    16921 gttggtcgcg cgtaaagcag gattacctga tgacggcgat tatgggcggg gcgtcgaata
    16981 ttactcagcc caccagcgat gtgaatagct ggacctggtc acgcacggcg aataccaaag
    17041 atgtgagtaa taaaattctc accaaccaga ccaacctgac ctcgacgttc tataccggtt
    17101 ctatcggtca tgatgtcact accggcgtgg aatttacccg tgaaacgcag actaactacg
    17161 gcgttaatcc ggtgacgtta cccgcggtaa atatttatca tcctgacagc agcattcatc
    17221 ccggcggcct gacgcgcaac ggcgcaaacg ccaatggtca gacggatacc ttcgcaattt
    17281 acgccttcga tacgctgcaa atcacccgtg attttgagct gaacggcggg atccgtctgg
    17341 ataattatca tactgaatat gacagtgcca ccgcctgcgg cggcagcgga cgcggtgcca
    17401 tcacctgccc aactggtgtg gcaaaaggtt ctccggtcac caccgtcgac accgccaagt
    17461 cgggcaatct ggtgaactgg aaagccgggg cgctgtatca cctgacggaa aacggtaatg
    17521 tctatattaa ctatgccgtt tcccagcagc ctccgggcgg caacaacttc gcccttgcgc
    17581 agtctggcag cggtaacagt gccaaccgca ccgattttaa accgcaaaaa gccaacacca
    17641 gcgagattgg caccaaatgg caggttctgg ataaacgcct gttgctcacc gccgcgctat
    17701 tccgtactga tatcgaaaat gaagttgagc aaaatgatga cggaacttac tcgcaatacg
    17761 gtaagaaacg tgtcgaaggc tatgagatat ccgtggccgg gaatatcact cccgcgtggc
    17821 aggtgattgg cggctatacc cagcaaaaag caaccatcaa aaacggcaaa gatgttgccc
    17881 aggatggttc ctcatcgctg ccgtataccc cggagcacgc cttcacctta tggagccagt
    17941 atcaggcaac cgatgatatc tctgttggcg cgggcgcacg ctatatcggc agtatgcata
    18001 aaggttcaga cggcgcggtg ggaacgccag cgtttaccga aggttactgg gtcgccgatg
    18061 ccaaactggg gtatcgggtt aatcgcaatc tcgacttcca gctaaacgtt tacaacctgt
    18121 ttgataccga ttacgtcgcc tcaatcaaca agagcggcta ccgttatcac ccgggcgagc
    18181 caagaacctt cttgctcaca gccaatatgc atttctgatt cagatgtggg gcgcaggccc
    18241 cactttttgg agaacttgta tgatgtacca cattcccggc gtgttatcgc cacaggacgt
    18301 cgcccggttt cgcgaacaac tggaacaagc cgaatgggtg gatggacgcg tcaccaccgg
    18361 cgcacaaggt gcgcaagtta agaacaatca acaggtcgac acccgcagca cgttatacgc
    18421 cgccctgcaa aatgaggtgc tgaacgcggt taaccaacat gctttattct ttgccgcggc
    18481 cttgccgcgt accctttcca cgccgctgtt taatcgctat cagaacaatg aaacctatgg
    18541 tttccatgtg gatggcgcag tacgcagcca tccacaaaac ggctggatgc gtactgacct
    18601 ttctgccacg ctgtttttaa gcgatccaca aagctacgac ggcggcgaac tggtcgttaa
    18661 tgacaccttc ggacaacatc gggtaaaact cccggcaggc gatctcgtgt tgtatccctc
    18721 cagcagcctg cattgcgtga cacccgtaac ccgcggcgta cgagtggcat catttatgtg
    18781 gatccagtcg atgatccgcg atgataaaaa gcgcgccatg ctgtttgaac tggacaacaa
    18841 tattcagtcg ctgaaaagcc gctacggcga aagtgaagag atcctgtcgc tgcttaatct
    18901 ttatcataat ctgctgcggg aatggtcgga gatctgatgc ttgaactgcc cgagttcgtc
    18961 cctggattag ctacacttaa ctgtacaagt attgatatgg ggaggtcgat atggcatccg
    19021 gttgggctaa cgatgacgcc gtcaacgaac agatcaacag tacaattgaa gatgcgattt
    19081 cccgcgctcg gggtgaaatt ccgcgcggcg aaagcctgga tgaatgtgaa gagtgcggtg
    19141 cccccatccc gcaggcccgt cgggaagcca ttcctggcgt gcgcttatgt attcattgtc
    19201 agcaggagaa agatttacaa aaaccagctt atacaggata taatcgcaga ggttcgaaag
    19261 acagccagtt acgttaacta caactggcga gatgcatagc gagtataatt tgtattttgc
    19321 gtggcattcc gtgcctttaa cgctgctgtg actgccacgc ggtataaaac aagttcataa
    19381 gtacaacaaa taaatggttt atcagtaggt tagatatcaa tcaatttatt tgaacaaggc
    19441 ggtcaattct cttcgatttt atctctcgta aaaaaacgtg atactcatca catcgacgaa
    19501 acaacgtcac ttatacaaaa atcacctgcg agagattaat tatgaaaact atcaatactg
    19561 ttgttgctgc tatggctctt tcaactctgt catttggcgt gtttgccgcg gaaccggtaa
    19621 cggcatccca ggcacagaac atgaataaaa tcggcgtggt ttctgccgat ggcgcatcca
    19681 ccctcgatgc cctggaagcg aaactggctg agaaagccgc ggcagccggt gccagtggat
    19741 acagcatcac ttccgccacc aacaacaata aattaagcgg tactgcggta atttataagt
    19801 aattcgtagg acggataagg cgtttacgcc gcatccggca acagatgcct gatgcgacgc
    19861 taccgcgtct tatcaggcct acaaaatccg aaccgttgga cggatcaggc gtttacgcta
    19921 cgtccagcac aagtatttcc ctgtctgttt gccgacagac gcatatgctc taaccctcat
    19981 tgatcctata ttacccttgt ttgcccgtcc gttactggac gggctttttt tagctggcta
    20041 actgctgaca gaaagcctgc aacgtctctt ccgacatacc aatctgccgc gccgcatcac
    20101 aaatggcctg ccagcttccc gcatccaatg gaatcccctg ctcctgccgt tcgcgacgcg
    20161 tgttcacttc ccactcgccc ggtagcaaaa tcggcttatc atcatcatgc ggcgaggctt
    20221 tcacccactc ggcaaaggct tcggtctgcg cgctacaatc cggcgcgccg aagagttccg
    20281 ggttgatgat gatagtggtc atgcagttaa gaatggcatc gggactggtt tgtaacgttt
    20341 cctgatgcgt cgttttaccg ccggaaagcg ccccgccaag aatttcacac atcgctgcaa
    20401 gggcgtagcc tttatgttcg gcaaaggtca acagcgaacc caacggcgac tcctgcatta
    20461 ccgccggatt ggtcgtcggc acgccgttaa cgtcaatcag gcaacctggc ggcacgggga
    20521 cgcctttatg ccaggcgacg cgggttttgc caaatgcaat ggcgctggtg gcgtaatcaa
    20581 gcaacagcgg gaaattatct ttacgaggga aaaccacaca gaacggattg gtgccaaagc
    20641 ggctgtcgcg accgtggaac ggcgcgacca ttggaatacc gaccacgcta acaaagtgga
    20701 tagagacaaa ccccgccgct gcacactgct ccgcccagta accgatacgg ccgatatgat
    20761 gcgagttatg tagcgccacg gcggcaatac cgtgctgatg cgctttctca atccccagcg
    20821 ccatcgcttc atgtgccgcg acctgaccaa atgcgcgatc gccatcgagc gtgactgccg
    20881 cccccgcctc tttaacgatt ttggcatgat ggttaatttg cagatgcccc tgactccagg
    20941 aacgcacata gcttgggatc atgccaatac catgagaatc atgccctgcc aggtttgccg
    21001 cgattaaatg atcggccact aatttcgctt cttgttcctc gctacccatc tgacgaaata
    21061 cagcctgaat aaaactgtgc agcgtctgag catcaaagcg atgaccactt tccatggtta
    21121 actcctg
//
LOCUS       DAFATA010000079        21087 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFATA010000079 DAFATA010000000
VERSION     DAFATA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.080"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..401)
                     /locus_tag="LGJ20_003410"
     CDS             complement(<1..401)
                     /locus_tag="LGJ20_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBK2387429.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQA"
     gene            complement(402..4915)
                     /locus_tag="LGJ20_003411"
                     /pseudo
     CDS             complement(402..4915)
                     /locus_tag="LGJ20_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(4939..5562)
                     /locus_tag="LGJ20_003412"
     CDS             complement(4939..5562)
                     /locus_tag="LGJ20_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBK2387430.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(5559..7247)
                     /locus_tag="LGJ20_003413"
     CDS             complement(5559..7247)
                     /locus_tag="LGJ20_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBK2387431.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQNKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEVGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(7396..10023)
                     /gene="gyrA"
                     /locus_tag="LGJ20_003414"
     CDS             complement(7396..10023)
                     /gene="gyrA"
                     /locus_tag="LGJ20_003414"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBK2387432.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            10170..10892
                     /locus_tag="LGJ20_003415"
     CDS             10170..10892
                     /locus_tag="LGJ20_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBK2387433.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(11020..14754)
                     /gene="yfaL"
                     /locus_tag="LGJ20_003416"
     CDS             complement(11020..14754)
                     /gene="yfaL"
                     /locus_tag="LGJ20_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBK2387434.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYRNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFSNDV
                     SEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            15450..17735
                     /gene="nrdA"
                     /locus_tag="LGJ20_003417"
     CDS             15450..17735
                     /gene="nrdA"
                     /locus_tag="LGJ20_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBK2387435.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            17824..18954
                     /gene="nrdB"
                     /locus_tag="LGJ20_003418"
     CDS             17824..18954
                     /gene="nrdB"
                     /locus_tag="LGJ20_003418"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBK2387436.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            18954..19208
                     /gene="yfaE"
                     /locus_tag="LGJ20_003419"
     CDS             18954..19208
                     /gene="yfaE"
                     /locus_tag="LGJ20_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBK2387437.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(19262..19912)
                     /gene="inaA"
                     /locus_tag="LGJ20_003420"
     CDS             complement(19262..19912)
                     /gene="inaA"
                     /locus_tag="LGJ20_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBK2387438.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            20091..20333
                     /locus_tag="LGJ20_003421"
                     /pseudo
     CDS             20091..20333
                     /locus_tag="LGJ20_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(20375..>21087)
                     /gene="glpQ"
                     /locus_tag="LGJ20_003422"
     CDS             complement(20375..>21087)
                     /gene="glpQ"
                     /locus_tag="LGJ20_003422"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK2387439.1"
                     /translation="IENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNHSTGKNI
                     GIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRIKNELEP
                     KMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADGIGPDYH
                     MLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDALYNKAGV
                     NGLFTDFPDKAVKFLNKE"
BASE COUNT         5012 a         5791 c         5095 g         5189 t
ORIGIN      
        1 gcctgacgct gttgccagta ttgcccccac acatctgcgc caatatgcaa ccgctgcggc
       61 gcaaaataga gcccacagga acgtaccagc gcggtatcgc gcgttatgct ctccccagcc
      121 tgacaacaat aaacctcttc cggggagttt ccgcgacact ggtagggctg ctccggttga
      181 tgggtatcct ccagccaggc gtaaacatac agtttccaca aactccctaa cggcgtggtg
      241 agcgagtcag gtaatgctga gactttggtc acgcctgatg acgacagctg atacagttga
      301 tcgtgctgcg caccgcgcag caccagttgt aacggcgtct cctccgcaag cgttggcaga
      361 gtaaccaacg ccaatagcca gacgattctg cgccagttca cttatttcac ctgcatcccg
      421 gtccattcac tccctgccgc aacactttgc tgcgcaggtg cataggaacg cacataacga
      481 gcaggaggca gaacgaattg ccctttttgc gagaagcgca gcaaatggcg gaaagtgacc
      541 gttcccgtca gttctttcac cggcaccata taggccagtt cgcccatttc attacgcgct
      601 ttttccagca attgcccctg ctgtttcgcg gcgttgggtt tattgactga aatgccccat
      661 gttgtgcgct caacgtcggc tcccggcggg agcggtactt ccacctgacc atagcgcaga
      721 actgcatcct gctcgctggt aagcgtaatt tcatcgagat acagcgcatc gctgtcaatc
      781 tcattgctgg tcaccggctg cagagtaaag ctcatctctt cttcaccggg gataagccga
      841 tacaactggc gttcaacggt caccggaatg ttactttgtt gagccgtttt tgccggttca
      901 cgccagcgga cctgcacatt ttgcggcgat aattcgtcac caaaagagag aatgtccggc
      961 acaccctgcc caacccaacg ccagtcttcg ccccctccag ttaacttatg tttagcccat
     1021 gcgcccgcag gcgcaggcaa aacaactgga ggcattgtcg ccatatattt cgccagccag
     1081 ttcatggcca gcgcgcgctc aatagtggat tgctcagcgg tcaaaccact taaaatagcg
     1141 gccgtctggg tagcgtcacc accgctattg agcagcacaa ccgtgtgcgc cagcggctga
     1201 ttgctgtttg ccgctttatc ccacgcatat tgctgaacct gtggcatcac cgtggacttc
     1261 aactgcgctt ttttcagcaa cgtcatggtt aagacgtttg ccaccgcatc tgccagtgga
     1321 gattccggtg tatcgaggat caggctgtca ttgatatcgc ggatgtcttc ctcagagaaa
     1381 tcttcatctt tagttccgcg ccgggcgata gcttcatcca accctttcaa caacgttttg
     1441 cacggcagat tcatctcctg tgcccacgcc agcaccagcg cccgatgcaa taacggcatg
     1501 ttatccgcct gctcggcata actgtcgagc atatgctgcc agtattccgg ttgttgcgtt
     1561 acgccgagcg cctggctggc ctgccagtcg gcgtaccatg cccatgccgt aaggaaggcg
     1621 tcaccattgc catcttcacc ccaccaggta aagcgcgctc cgggccccgc cagttgcatc
     1681 agccgcagac ggttatcctg aatcatctga cgaatgtcgt tagcggcggc actttgatga
     1741 tcggcaagcg aacgccaggc gagactgagc gggatcagac ggctaccggt gttgatcacg
     1801 ccaccccatg gtccatcgac taacgcatca agattgttgc ggaaaatctc ctgcggcgtt
     1861 tcgctacttt gtagccggat attgctagcc tgctcgggca acatcagcgc gttctcgcca
     1921 ccgccgagca tgacattttt ctgctgttca acgggccagc tgttatccac aaaagacagt
     1981 tttgtgctaa tgctgtcctg cacttgccca ttttgttgca gttctgcgct taacaagcca
     2041 gattgctgaa tgttctgcgc cagcgaaata taattcgccc ctttgtgcag cgtcagcgtc
     2101 tggcgcatct cagcgcctgc aaatttagtc accagcgcca ccggttcgtt atcctgctga
     2161 ctgaagataa acagtcctgc cgccggtttg tcgcccacgc gatacaccgt tggcatactc
     2221 cacttcatgt agagattttt ttccgaacgc agataagcac gcccctgccc gaccagcccg
     2281 tcgccgttca tcccacgcgc ggtgatacgc cagcgggtta acgaatcagg catcaggaac
     2341 gtgaaatacg ctttgccttg tttatcggtt gtgagtgacg gcatccatgc cgcggtatcc
     2401 acctcttcac gccgtggacg ttcaagcatt tttactcgcc gctcgctgcg gttagttgcg
     2461 ccaggcgcaa ccggctcgct ggagagcgcc tggtcgtagc tgataaacga cagactggag
     2521 ctggtacgca cattgttacg ccccagcgga tagaaaaatt tgccgatatt cggcgcgatt
     2581 tctggttgca gcgcgtagat catttcatcg accacgccta ccgttagctg cgcagaaaca
     2641 ggtttacctt tcagcgacga ggttaattcg acattgacca gttcaccagg ctggtaatgg
     2701 gttttgtccg ttttcacccg gatatcaagc tgaggaacgg caactttgat cccggcgttc
     2761 tgaaaactgt actgaccatt acgggtatac agcaccgaaa aagtgatgtt aggcgcaaag
     2821 gaattgctca ctggaacccg ggcttcatac tgggtatcgt ttaaacgttg tagcgttagc
     2881 cagtttgccg gatgcgaaag cagcgactgc tgttccacgc gatcgcgttc cagcgtcaat
     2941 aatgcttcat caattggctc cggaaaggta atcagcatct tcgcggtttc gcctggctgg
     3001 tacagcgttt tatccgccac gatatctacc gtacccgtat gcgccgtgct gcccttaccg
     3061 ctgacggcat aacttaaccc agcgagaatt aagccgtctt tatcgcgtaa tgtcagattg
     3121 tagttgccag gtttagcgaa attgacggta aaggatttgc cgcctgacgg tagctctccg
     3181 ctatggctcg tgcggtcttc gagacgcaac cattcatacg taacaggaac ctgttttgaa
     3241 gattccagcg cggcataacg gaacacaacc gactcgccgc tattactgta ttgtgcggca
     3301 gtacttaatg agtaatgcgc cagaccgcgt tcaatgagga tctctttggt ggtggtgaca
     3361 cgatacgccg cgccgtcact ggcggagacg gttaacaaat aacggctcgg tttatcggcg
     3421 gcggggagat ttaacgccac atgaccgctg gcgtcggaca ccgtttcgct gccttccagc
     3481 gacacgggga aacgtccggc ataacgcaaa tcgttaccga ccattgataa ttgctgagcg
     3541 cgcaaactta actgcacgcg ggcatttttt accggctcgc catccgggta gagcagttgc
     3601 agtttgccgc tgaccgcttc gccagttttg aactcttttt tgtcgagagc taaaccaatc
     3661 tcgaaatgtg gcttgatgta gtttgccacg cgaaaactgc tgctatagac ctgattgcgg
     3721 taagcaagac gtaactcata acctccggca acggcatttt ctggcaggcg gaaacttccc
     3781 tgcccgccat tgcgcgcatc cagcgtgaca tcgacggttt gcaacagact gccgttagcg
     3841 tccagcaccg aaagcttcgc cggggcgctg acgatggggg atgaatgcaa cggatcgtgg
     3901 aactcgcggc ccatcacttt aacatcgaca cgatcgcctg cgcgatatag cgggcgatcg
     3961 gtaaaaatat acaagcgggt gttgtagatt tcgctttcgt agaagaagtt ctcggagaca
     4021 aaaacaccgc cttcagcatc cttacccaga atgtatgaac gttctggcga tatatgttgt
     4081 aactgcaagg taccgctgtc atcggtcaca ccgcgggtca tcacgccaag accgtcagtc
     4141 cacaagatct ctgagccggg cttcgcttca ccctgttttt tacccgcggt ccacaccaga
     4201 agctcgttgc ctgacacttt gctaagcgcc acggtatcgg aaacaaacac caccgtcgtc
     4261 gcccgatacc caccaaccat cgcctcgacg aggtacagtc ccggctcttg ttggccgaga
     4321 ggaatataaa tgttacccgg ctgcggcgag atgaaattgc tggatgcgcc ttccagtttt
     4381 accccttgct gcggctcgac cggtttagcc tgccatagtg gataacggaa ctgttccacc
     4441 aagggatatt tttcagcggg gaaaactggt tgttctgtac ataacgggaa ggtttaataa
     4501 tggcattgcc gagatgtaat tcgggtaatg cctgagtcac attctgccgt gactgagaag
     4561 agaaagtacg ctgcattacg cggcgagatt tgccgtacca gttatcccac agccaggtca
     4621 gcgtattgtt cagcccgtcg cccagatatt gcggttgcac cacaatgcga tgcaggtttt
     4681 tctgctggcg caaaaatgcc atcgggtcag gaatacgata caggcgaacg tccacgccgc
     4741 cgtactcttc catctgatag cgccgataat cacgcccggg cgcttccagt cgcactttcg
     4801 cctcttcact gctgctaaaa ctgctgtcag caagcaaaaa gaatgttccc ccggcgggcg
     4861 gcgcatagtt actggaagga agcgaatcat cagcattagc aagccctgtt cccacaagag
     4921 acagggacaa gcacgctgct atcgcgccag aaaatttaaa cgataaatgc caatgaaatt
     4981 gggattggaa tcgttgggta tccatcgggt gtccttccat gtcataagtt gttgcagact
     5041 gactgcgcgc attccgttgt cagttttcgt ggcgcttccg gtgtggtaga tgacgtaacg
     5101 ccccatccag accattaagt gctgggcatc gccctgatcg aaaaaaatca tatcgccagg
     5161 cagcgcctgg tttatgtcct ggccaataaa ctggctgttg tactgaatca aattaatcgc
     5221 ggtcacgtag gggccggttt tcccgttccc ctgattccag ttttgcgcca gttgacgctg
     5281 tccaggtgtt agcgtcatct ctggcggcaa atactggcta gagaaaccgt tactttttag
     5341 ccatttacta tcgtgaactt tcagcgtctc gttcgccgca aatcgcacca ggcccgcaca
     5401 atcctgctga taccagcgtg gacttggccc ctggcggagc tgttcttgtg caatgcgtac
     5461 aaaccaggcg cgaaacagcc cggattgttc gacattcagc atttcgctat gggcaacaac
     5521 acaacacagc cagcaaatca gcgccagcag cccgtgcctc atagcggctg ccaggttatg
     5581 ggtagccact gccaggcggc accgggttcc atctgggcca gcttcatgac ataacgcggt
     5641 tgttgagata aagcgtccag cttcggcatt aataaagttt gtgcggcgtt ataaaaaacc
     5701 ggttcgagat tcttcggcag actggtcaac gtttcgttac gcagcagttt cgccacgcct
     5761 tgtggattga tatagagcgg aacgatgcca tcagtgggta ttacatccac cattgccggg
     5821 cggtttttat tcagtgtttg cagggcgtta ttaaccagcg tgtcatcgag ggagaaaagc
     5881 agcgttttgt tttgcatcgc cagcgacacg cggaaaaaat aatccgacat taattgatcg
     5941 ggttgcgccg cctgcgcttt cggatactga ccgtatcggg aactcacttc gcgacgccag
     6001 atttgcgctt cgccctgctg agtctggctt atcggcaaaa caccttctgg cgctttgctt
     6061 tcgtgcgcac caatattttg cgtaaacagt ttccctggca attgcgcctg ttcggcagtg
     6121 ccatcaaact gaccgacaaa cagcggggtt tgcaattttg agtctgcata ccagcacagc
     6181 cccgcggcac cgtctaacgc cccattcaac ttgtcgtttt cctgactgat gtgcgaaagc
     6241 atctcttcgg caataccgtg tgaatacggc accaccacac agaagctggc cccggcaggc
     6301 atactgttcc agaccggcgt aaaatcgaaa ctggtatcta cgctggcgga ttcatcattt
     6361 aacgccacaa aactgtgcca gccgtcgtta cccacttcga agcgtacgcc agcaaaagaa
     6421 ggcattaacc gctggtagcc aaaacccagc aacctggcgc tgaccacgat gcgctggcgt
     6481 actggcgttt tttcagcagc acgctcttcc aggccaaagc ttgcttgcca gcgttttttg
     6541 ccgctcaaca aatcacctgc gatcgccgtg gcttcggtat cctgctgatc atctttaaac
     6601 aacatatccg tgctggaaaa caccagcatc ttgttctgat aagtcgcgaa catcagggcg
     6661 ttattgccgt tatagcgcaa ctgataaaca ggaacagttt cactatttat cttgatgcta
     6721 ctgatttccg ttttgcttaa ctggctgtcg ctggtagcgg caaacaacaa tggctccagc
     6781 agtttgctta aaccactgcg ctggatcagc accatataat gtgaaagatg gccttgttta
     6841 tcgtgccaca gcgctgcctg cgcgggctga tctaagagtg acgaaaagag cttatctttc
     6901 agcgtgagat cgtgttcata gacaatacga cgaatgctgc cttcaatgcc cagacgatcg
     6961 gcatgattct gataatagaa aacgaaatct tcgctcagaa catcgtggag aaacggaatg
     7021 gtgaggagat ctttgggaag ctggctcaga gagtcgctgt cgagaaagag gtccggctca
     7081 ttgagatcga tttgcagatt gttgtgcacc accagcggcg acaacgtttt ttctggccca
     7141 ctgccagcat attgcaacgc ccagacgcca gcggaaagca gtgctattgc gccaaaacct
     7201 acaagaccat agaaccgcca gcctttcgcc tttttttcac cactcattgc cacattcctt
     7261 gtgtatagcc agccattttt tacgggcaca gccaaacttt accgtgccct aatacgacaa
     7321 aagcccagac tttgcagcct ggacttttca attcaaacaa gggagatagc tcccttttgg
     7381 catgaagaag taaaattatt cttcttctgg ctcgtcgtca acgtccactt ccggagcgat
     7441 ttcatcgtcc ccttccgcgg cactgccatc gatggtatcc agatcttcct cgtcaaccgg
     7501 ttcagcaaca cgttgcagac ccactacgtt ttcatcttcc gcagtacgga tgaggatcac
     7561 gccctgggtg ttacggccta cgatgctgat ttccgaaacg cgagtacgta ccagcgtacc
     7621 ggcatcggtg atcatcatga tctggtcgca gtcatctacc tgtaccgcgc caaccactaa
     7681 accattacgt tcagtaacct taatggagat aacccctttc gtcgcacgcg acttggttgg
     7741 gtattccgcc actgcggtac gtttaccgta accgttttgc gttgcggtga ggattgcgcc
     7801 atcgccacga ggcacgatca gagagacgac tttatcgcct tcacctaagc gaataccgcg
     7861 aacaccggtg gtgttgcagc ccatcgcacg gacagaagac tctttaaagc gcaccacttt
     7921 accttcagcg gagaacagca ttacttcgtc ttcgccgctg gtcaggtcaa cgccgatcag
     7981 ctcatcgccg tcaaccagtt tgatcgccac tttaccggcg gtacgcagac ggttgaactc
     8041 ggtgaggaca gttttcttca cggtaccgtt agcggtcgcc atgaagactt tcacgccttc
     8101 ttcaaactcg gtcactggca ggatcgcagt gatacgttcg tcctgctcca gcggcagcag
     8161 gttgacgatc ggacgaccgc gcgcgccacg agtggcttcc ggcaactgat aaactttcat
     8221 cgaatagacg cgaccacggc tggagaagca cagaatatgg tcgtgagtgt tcgccaccag
     8281 cagtcggtcg ataaagtctt cttctttaat acgtgcggca gatttacctt tcccgccacg
     8341 acgctgcgct tcgtattcag aaagcggctg atacttaacg tagccctggt gagagagcgt
     8401 cactaccaca tcttcctgag tgatcagatc ttccaggtta atgtctgcgc tgttggcggt
     8461 gatttcagta cgacgtttgt caccgaactg ttcacgaacc agctccagct cttcacggat
     8521 cacttccatc aaacgatcgg cgctaccaag aatacgcaac agttccgcga tctgatccag
     8581 cagctctttg tattcgtcga gcagtttttc gtgctcaagg ccggtcagtt tctgcaaacg
     8641 cagatccaga atcgcctgag cttgctgttc ggtcaggtag tacagaccat cacgcacgcc
     8701 gaactctggc tccagccatt ccggacgcgc agcatcgtcg ccagcacgtt cgagcatcgc
     8761 ggcaacgttg cccagctgcc acggattagc aaccagcgca gttttcgctt ctgcaggcgt
     8821 cggcgcatga cggatcagtt cgatgatcgg gtcgatgttc gccagcgcca cggctaatgc
     8881 ttcaaggata tgagcacgat cgcgagcttt acgcagttcg aaaatagtac gacgggtcac
     8941 cacttcacgg cggtgacgaa caaacgccgc gatgatgtct ttcaagttca tgatcttcgg
     9001 ctgaccatgg tgcaatgcca ccatgttgat accgaaagaa acctgcaact gggtctggga
     9061 gtagaggttg ttgagcacaa cttcaccgac cgcatcgcgt ttcacttcaa tcacgatgcg
     9121 cataccgtct ttgtcagact cgtcacgcag cgcgctgatg ccttccacgc gtttttcttt
     9181 taccagttcc gcaatcttct cgatcaggcg cgctttgttt acctgatacg gaatttcgtg
     9241 gacgataatg gtttcacgac cggttttggc gtcaacttcc acttctgcgc gagcgcggat
     9301 atacaccttg ccgcgaccgg tacggtaagc ttcttcaata ccgcgacgac cgttaatgat
     9361 tgccgccgtc gggaagtccg gccccgggat gtgttccatc agcccttcaa tgctgatgtc
     9421 ttcatcatcg atatacgcca gacaaccgtt gatgacttcc gtcaggttgt gcggcgggat
     9481 gttggttgcc atacctacgg cgataccgga agaaccgttc accagcaggt taggaatttt
     9541 ggttggcatg acgtcgggaa ttttttccgt gccgtcatag ttatcaacga aatcgaccgt
     9601 ctctttttcg agatcggcca tcagttcatg ggcaattttc gccagacgga tttccgtata
     9661 acgcattgcc gccgcagagt cgccgtcgat ggaaccgaag ttaccctgac cgtctaccag
     9721 catgtaacgc agcgagaatg gctgcgccat acggacgatc gtgtcataaa ccgccgagtc
     9781 accatgggga tggtatttac cgattacgtc accaacgaca cgggcagatt ttttataggc
     9841 tttgttccag tcattgccta gtacgttcat ggcgtaaagt acgcgacggt gtaccggctt
     9901 caggccatct cggacatctg gcagcgcacg gccaacaatg accgacatcg cataatccag
     9961 ataggagctc ttcagctctt cctcaatgtt gaccggtgta atttctctcg caaggtcgct
    10021 catctaaccg ctatccctct actgtatccc ggattcaaag gtcgcaaatt ataacacagc
    10081 cgcgcagttt gaggtaaacc tatacgcttt attcacatcc aatgcctgat atactcgttt
    10141 gtcttgccaa ttacggagta gaagtgccaa tgaatgccga aaaatcgccg gaaaaccata
    10201 acgtagacca cgaagagatc gctaaatttg aagccgtcgc ctcccgctgg tgggatcttg
    10261 aaggtgagtt caaaccgttg caccgcatta acccgctgcg tctgggctat attgccgagc
    10321 gtgctggcgg tttatttggc aaaaaggtgc tcgatgtcgg ttgtggcggc ggcattctgg
    10381 ccgagagtat ggcgcgcgaa ggcgcgacgg tgaccggtct ggatatgggc tttgagccat
    10441 tgcaggtggc aaaactgcac gcgctggaaa gcggcattca ggtggattat gtgcaggaaa
    10501 ccgtggaaga gcacgcggca aaacatgccg ggcagtatga tgtggtgacc tgcatggaga
    10561 tgctggagca cgtccccgat ccgcagtcag tggtcagagc ctgtgcgcaa ctggtgaaac
    10621 caggcggcga tgtctttttc tcgacgctta accgcaacgg caagtcatgg ctgatggcgg
    10681 tggttggcgc ggaatatatt ttgcgcatgg tgcccaaagg cacgcatgat gtgaagaagt
    10741 ttattaaacc ggcagaactg cttggctggg tagatcagac aagccttaaa gagcgacata
    10801 taaccgggct acattacaac ccgctcacta atacttttaa actcggcccc ggcgtggatg
    10861 tgaactatat gctgcacacg cagaataagt gaggttgttg tttggccgag ccaatgcctg
    10921 atgcgacgct tgccgcgtct tatcaggcct acaaatgctc cccgtaggcc ggataaggcg
    10981 tttacgccgc atccggcaac cgtgccgact agacagtgat taccatttca ccgtcatcga
    11041 caaaaaccct gccgtctggg caaaatcatc actccctttc tgccacgcca cgctgccgcg
    11101 cagggacact cgctgactga tattgcccgt gactcccact tttatttcac cccgttgctt
    11161 caccgcatcg tcactgatag tgctgccatc ctcttcaatt tccgtcgaat ggggatcgtg
    11221 ataataattc agatctaatg ttggtatgac atgaacagcg gtacgccatt cgctgtgtaa
    11281 acccagccgc gtctgaatat catcaccctg cgattgtgac acgcgcgcac ggttagcggc
    11341 ggtaaaatca tcctgctgca cgccctgata aatcacctgc gcctgcggtt caatcaccac
    11401 accacgcccc ggtaaccact gataccccgc ctccagcgag gcgataatcc ccgacgagtg
    11461 gtaatgatct gtgccatctt cttgttcgga aacatcattg ctaaaccacg cgtattgcag
    11521 ccagctatcc aaccaggccc cctgcttctg attaccgtgc tgataccagc ttgaggtcag
    11581 cccaacggca taaccgtggt tctggttatc ggcgcgagtt ccggtcatat tcgagcggct
    11641 ctcgccctga ttatcactgt agccaccaac cgccccaagc atccactcgc catcatcgcc
    11701 ccagcgcccg ctaaacaggt caccgctaag ctgcaccgta gaagtgtctt catgctgagc
    11761 cagttgccct gccgccgtgt aatgataacg tccgccgata acacgtaaat tcagcgtctg
    11821 accattgcca cctgcatgat cgcgtcgctc catcataaac gcctgatttg ccgcccgcag
    11881 attattgcga taaccgccaa ctttggcatt cagcaccggc tggtaagcgg ggattggatc
    11941 gggtgttggg tcaggatcgg gtgttgggtc agggtctgga tcaggtggcg atggcggcgt
    12001 tacttcttgc gatcgcagat accagtcgtt gttatcttcc accagcgtgt agtcatacgc
    12061 tcccatattg acgtagccgc tgcctgccag actgaactgc gcattgtttt gaaactgcgt
    12121 gggatcagct gcgaaatcaa ccactttaat gcctgtcgat gtcggctcac caatccctgt
    12181 aatggagtta accaccacag ttgtgttgcc agcagtatta ccgttcatca ccaattgatc
    12241 gcttaccgag tcatcgccgt ttaattcgct atcgagcagt agcgtaccgc cgccggtata
    12301 atcaccgttt accgtcagcg tatcgccagc gacgccattt tgcaggctaa ccgcaccgga
    12361 cgtattggtc agattgccat taaccgtcac gttggaaaga taagtcaggc tgtcagtttc
    12421 aaggttggta ttaagctcac ctgtcaacgt cacaccatcg gtggcattca ccagcgtccc
    12481 atcgccgtta aatagtgagg cattaagggc gacggaagta tcctgcccgg tcaggcgcaa
    12541 aaccgtacca tcgctgatgt cgatagtgcc gctggaaata gcatcaattg actgaatatc
    12601 ctgatccgcg ccagtaacga acgttgcccc atcaccaacc cacagtgacg aagcataagg
    12661 aaggatatca gcaacatcac ctttcagggt gccttcttcg acacgtaccg ccgactgcgt
    12721 tgtaccgctg gcggtcagct ccagtgtacc agcccccgtt ttagtcaatg tgctaccctc
    12781 atcctgatgc tgcccgctgc tgtcagccat cagtgcgccc cactgcgtgt ctacccccgc
    12841 atcaactgcc acttcaccgt cggcgcgcat ttcaatatca cgtccgtggc ctgccgcatc
    12901 tgaacgcgtg tcaacgtcgg aggtaatctc catcacgctt tcttgttgct tatcggtaaa
    12961 gataacctgg cggttatagt gggtatctcc aagctgcgag ttgtcgctca ccatcagcgt
    13021 accggaggcg atttgtgttg tgcccaaata actattattg tttgccagac ttacctgccc
    13081 gtcgctcgca tcgaccacca cgtagacgcc atctttcccg tcgccgccga tatgcaaatt
    13141 gtcgccacct gcaagatcta ccacatcctg actgccgata accgttccgc tgctgccact
    13201 gacttcaagg ccatcgttgt atgatgtgcc gctctgccag gtggagaaat cagagagatc
    13261 gagcacaccg ccgtttaaca cgatcgactg cggatcgtcc tggagagcgg taagatctgc
    13321 cgcgtcgcct tccagcgaaa gcaccgcacc atcatcaacg actatatcgc cggttagcgc
    13381 catcgactgc gcccctgcca gcacgtagct gccgttttgc gcaatggtga gctgaccagc
    13441 accttcgatg atgccagcaa aactgccctg attaacagta acgttgccac cagcatcgat
    13501 atttaaagtg ccattctgaa agcccgtcaa tgcgtgcaca aaagtttgtt gggtcgagcc
    13561 aacgtttagc tcagcctgat tctgatactg atcaatactc cctatcgtca gaccgtagca
    13621 gtcttgcgga tcgtcctggc aatgcgtatc gccgacattc atcagggagt tgctgcggcc
    13681 cagggtaact tcaccgtttt caatctgcat ctcaccagta aagtcattgt tatctgcatt
    13741 aagtaccaga tcgccggaac ctgttttggt gatcaacccg gtaccagcaa tagagtcaac
    13801 agctccgtca ttctctgtat tgccaataac cagcgttttt ccgtcggcaa tatcaaaggt
    13861 aacttcactt aagccgagat acataaagcc acccgccgca gtggaaggac catctccata
    13921 gcctgctgcg ctgttgttct catccactaa cacgccgccg ttctggctgt agctgtcatc
    13981 aacagaaata tcaataagat agggagccgt cgcgctattg gtatatatcg ccccgccata
    14041 accttcggca gtgttatttg taaaggcggt gttatttatt atcgtataac ctgaaggatg
    14101 cttgctgtcg ctattattat cggtaacatc gattgccccg ccatcgccat cactgtaact
    14161 tgttgatgta tatgcctggt tgttatcaaa aataacatca cttaaataaa cgtcattatt
    14221 aatggtataa attgcgccac ctttgccatc attagcgatg ttattgcgaa acatggcgtt
    14281 agtgacacgt aaatcgacgg caccagtatc gttagtacca gaagaataga ttgcgccacc
    14341 atagccgcct gcgacgttac cggaaaaaat aacatcagta agattcagcg ttgagttttc
    14401 tttagcaaat atggccccgc cgttattata ttctcctgta acggtgttat tggcaaacag
    14461 agtcattcca gtttcatttt ctggtaatag tgaaaattcc gctccttgtt gcaaaaatac
    14521 ggctcctccg ctggcattat tggtcatatc cgaaaaaacc agccattgcc catcggcgac
    14581 actccagtcc tgcgtaatgc ctgaaaggct ttgcctgctg gcgtgacaac tcgctttgat
    14641 atcatatccc tggcataaat caatggccgc cgcgacaccg ttagcagaga aaagagatgc
    14701 aatcattgac gggagtagag ataaatactc cttgcgtaga aagataatcc gcatattaat
    14761 ctgaccatca ttttatataa gacggcatat ttaaacgcat tatacaatat gggttatctt
    14821 tcttttgtaa attgtccaac ccgatgcaat ggctattttg ttattatttg atgaataata
    14881 tcagtgcgtc ataattcaag ttaataacct taagggatac cagttatatt taaactaaat
    14941 taaagtaatg aataattttc ttataatata aggtaaatta acaaaatggc ttcgcattta
    15001 acaataaccg aatagaaaac aaccatttcg acatcaacaa tcgcttacat tcgcttatat
    15061 attgaccaca actgatacat cagattatgt gatgactcgt gcttagatca atttttgcaa
    15121 tcattagcaa aaagattaat aaaccatcta tatcaattta tccaacctat tctgccgttc
    15181 aagaaatcgc cgaacagtta tttttaacaa atttttctct tcccattgac tttcccggac
    15241 accttgtctg acctaaggtg cgcgaaagcc actttttcct tcctgagtta tccacaaagt
    15301 tatgcacttg caagagggcc attttcacac tatcttgcag tgaatcccaa acataccccc
    15361 tatatatagt gttctaagca gcttcccgta ctacaggtag tctgcatgaa actattgcgg
    15421 aaagaattcc aaaaacaggt acgacataca tgaatcagaa tctgctggtg acaaagcgcg
    15481 acggtagcac agagcgcatc aatctcgaca aaatccatcg cgttctggat tgggcggcag
    15541 aaggactgca taacgtttcg atttcccagg tcgagctgcg ctcccacatt cagttttatg
    15601 acggtatcaa gacctctgac atccacgaaa ccattatcaa ggctgccgca gacctgatct
    15661 cccgtgatgc gccggattat cagtatctcg ccgcgcgcct ggcgatcttc cacctgcgta
    15721 aaaaagccta cggccagttt gagccgcctg cgctgtacga ccacgtggtg aaaatggtcg
    15781 agatgggcaa atacgataat catctgctgg aagactacac ggaagaagag ttcaagcaga
    15841 tggacacgtt tatcgatcac gaccgtgata tgaccttctc ttatgctgcc gttaagcagc
    15901 tggaaggcaa atatctggta cagaaccgcg tgaccggcga aatctacgaa agcgcccagt
    15961 tcctttatat tctggttgcc gcgtgcctgt tctcgaacta cccgcgtgaa acgcgcctgc
    16021 aatatgtgaa gcgtttttac gacgcggttt ccacatttaa aatttcgctg ccgacgccaa
    16081 tcatgtccgg cgtgcgtacc ccgactcgtc agttcagctc ctgcgtactg atcgagtgcg
    16141 gtgacagtct ggattccatc aacgctacct ccagcgcgat tgtgaaatac gtttcccagc
    16201 gtgccgggat cggcatcaac gccgggcgta ttcgtgcgct gggtagcccg attcgcggtg
    16261 gtgaagcgtt ccataccggc tgtattccgt tctacaaaca tttccagaca gcggtgaaat
    16321 cctgctctca gggcggcgtg cgcggcggtg cggcaacgct gttctacccg atgtggcatc
    16381 tggaagtgga aagcctgctg gtgttgaaaa acaaccgtgg tgtggaaggc aaccgcgtgc
    16441 gccatatgga ctacggggta cagatcaaca aactgatgta tacccgcctg ctgaaaggtg
    16501 aagatatcac cctgttcagc ccgtccgacg taccggggct gtacgacgca ttcttcgccg
    16561 atcaggaaga gtttgaacgc ctgtatacca aatatgagaa agacgacagc atccgcaagc
    16621 agcgtgtgaa agccgttgag ctgttctcgc tgatgatgca ggaacgtgcg tctaccggtc
    16681 gtatctatat tcagaacgtt gaccactgca atacccatag cccgtttgat ccggccatcg
    16741 cgccagtgcg tcagtctaac ctgtgcctgg agatagccct gccgaccaaa ccgctgaacg
    16801 acgtcaacga cgagaacggt gaaatcgcgc tgtgtacgct gtctgctttc aacctgggcg
    16861 caattaatag cctggatgaa ctggaagagc tggcaattct ggcggttcgt gcacttgacg
    16921 cgctgctgga ttatcaggat tacccgatcc cggccgccaa acgtggagcg atggggcgtc
    16981 gtacgctggg tattggtgtg attaacttcg cttactatct ggcgaagcac ggtaaacgct
    17041 actccgacgg cagcgccaac aacctgacgc ataaaacctt cgaagcgatt cagtattacc
    17101 tgctgaaagc gtctaatgag ctggctaaag agcaaggcgc gtgcccgtgg tttaacgaaa
    17161 ccacttacgc gaaagggatc ctgccgatcg atacctataa gaaagatctg gatgccatcg
    17221 ctaatgagcc gctgcattac gactgggaag cattgcgtga atcaatcaaa acgcacggcc
    17281 tgcgtaactc cacgctttct gccctgatgc cgtccgagac ctcttcgcag atctctaacg
    17341 ccactaacgg tattgaaccg ccgcgcggtt acgtcagcat caaagcgtcg aaagacggta
    17401 ttttgcgcca ggtggtgccg gactacgagc acctgcacga cgcctatgag ctgctgtggg
    17461 aaatgccggg taacgatggc tacctgcaac tggtaggtat catgcagaaa tttatcgatc
    17521 agtcgatctc tgctaacacc aactacgatc cgtcacgttt cccgtcagga aaagtgccga
    17581 tgcagcagtt gctgaaagac ctgctcaccg cctacaaatt cggggtcaaa acgctgtatt
    17641 atcagaacac ccgtgacggc gctgaagacg cgcaggacga tctggtgccg tcaatccagg
    17701 acgatggctg cgaaagcggc gcatgtaaga tctgataatc ccccagcctt tgcaccaaac
    17761 cgtcccctct ccccttgcgg gagagggtta gggtgagggg taataaaacc acaggacaca
    17821 ctcatggcat ataccacctt ttcacagacg aaaaatgatc agcttaaaga accgatgttt
    17881 tttggtcagc cggtcaacgt ggctcgctac gatcagcaaa aatatgacat cttcgaaaag
    17941 ctgatcgaaa agcagctctc tttcttctgg cgtccggaag aagttgacgt ctcccgcgac
    18001 cgtatcgatt accaggcgct gccggagcac gaaaaacaca tctttatcag caacctgaaa
    18061 tatcagacgc tgctggactc cattcagggt cgtagtccga acgtggcgct attgccgctt
    18121 atttctattc cggaactgga aacctgggtc gaaacctggg cgttctcaga aacgattcat
    18181 tcccgttcct atactcatat cattcgtaat atcgttaacg atccgtctgt tgtgtttgac
    18241 gatatcgtca ccaacgagca gatccagaaa cgtgcggaag ggatctccag ctattacgat
    18301 gaactgatcg aaatgaccag ctactggcac cttctgggcg aaggcaccca caccgttaac
    18361 ggtaaaactg tgaccgttag cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg
    18421 agcgttaacg cgctggaagc gattcgtttc tacgtcagct ttgcttgttc cttcgcattt
    18481 gcagaacgcg aattgatgga aggcaacgcc aaaattattc gcctgattgc ccgcgacgaa
    18541 gccctgcacc tgaccggcac ccagcatatg ctgaatctgc tgcgcagcgg cgcggacgat
    18601 cctgagatgg cggaaattgc cgaagagtgt aagcaggagt gctatgacct gtttgttcag
    18661 gcagctcaac aggagaaaga ctgggcggat tatctgttcc gcgacggttc gatgattggt
    18721 ctgaataaag acattctctg ccagtacgtt gaatacatca ccaatatccg tatgcaggca
    18781 gtcggtttgg atctgccgtt ccagacgcgc tccaacccga tcccatggat caacacctgg
    18841 ctggtgtctg ataacgtgca ggttgctccg caggaagtgg aagtcagttc ttatctggtc
    18901 gggcagattg acgcggaagt ggacaccgac gatttgagta acttccaact ctgatggccc
    18961 gcgttaccct gcgcatcact ggcacacaac tgctgtgcca ggatgaacac ccttcccttc
    19021 tggcggcgct ggaatcccac aatgtggcgg ttgagtatca gtgtcgcgaa ggttactgcg
    19081 gctcctgtcg cacccgtctg gttgcaggtc aggttgactg gattgccgaa ccgttagcct
    19141 ttattcagcc gggggaaatt ttgccctgtt gttgccgggc aaaaggcgat attgaaatcg
    19201 agatgtgaat tggttgtagt gccagataca acgcttatgc gtcttatctg gcctacaacg
    19261 attacatggc gtagtaatac gctttcacct gctcccagtc cgctttgggg attggctcca
    19321 gatatttttc cagctggcgg aagtcatgat taatcgcttt atcgcgacgc aagcggcgac
    19381 gacttttctc caggtcaaga aaaccagctt ctgcattacc ttctgttttc acatagatat
    19441 ggcgaacata acaacagcca tgctgacgat taatgctatg cattttctta aacgccagcg
    19501 ccactgcttt caacatggct tgccgtactt cgtcagaata aggcgatact gcatgctggg
    19561 cataccagtc agcaatgctg atgaaccccg ccatatcttc agtcaccagc aacgctcgcc
    19621 attcaccctc aattttcacc gcttcaccaa aaacgatctt cggcacgatg acaccggccc
    19681 gttccagttc tttaattacc gcaacctcac ggacaatcgt tggtcggccg aacggataac
    19741 gtacggaatg aaacagatga tgcgtcatgc gctttacata cagctttttg ccgttgcgct
    19801 cgacgcattg caccccgctc ataccattac ggcgatagtt aggctcttca acccagtcgc
    19861 cctctgttgc ccaccagtga ttaaattcgt cgtactttgc tgaaactgcc ataccctatc
    19921 gcctgttatt tttattaacg acaatgacta taggtggtta cctgaggaaa atcttaatga
    19981 aacgtgtcgt attaatgaat ttcttttgcc actactagct tgacaccgct tttacccttc
    20041 atttgcagcg catagattcc accagccact accactaata gtgcgcccag cagataaaat
    20101 gtgaatttaa tcaggcggat acagcgatga attttattcg acagggatta ggcatcgcct
    20161 tacaaccaga gttaacgctg aaaagcattg caggtgaatt gtgttccgtt cctctcgaac
    20221 caactttcta tcgacagatt tcgttgctgg ctaaagaaaa gccggtagaa ggcagtccac
    20281 tgtttttact acaaacgtgt acggaacaac tcgtggtgaa tggaaaaatt tgatgtgaga
    20341 caggatggcg catgaacgcc atcctgcagt agcattactc tttattaagg aattttactg
    20401 ccttatcagg gaaatcagta aacagcccat ttacacccgc tttgttatac agagcatcat
    20461 ataactgatt cacatcagta gtgtattcag gcagtttatc tgaccgcacg gtataaggat
    20521 gcactaccag tttattctgc tgagcatctt gcaccatgcc agtgagtttg atattacccg
    20581 gctgcgatgt ttcctcaatc aacatatggt aatccggccc gataccgtcg gcatattccg
    20641 ccacctgttt catagcaccc ggcttaaaca tccagtcgta gttgtaatta acccagcttc
    20701 catccggctg tttctgctgc gtttcattcc agtcggtata ggcaatcaac tgtaccagat
    20761 tgagatccat gcccatttta ggttccagct cattcttaat acgcttcagc tcatcagcat
    20821 caaaacattg caaataaact ttgtcgtcct taccggtgta accatatttc ttcagcactt
    20881 ccagcgtttt tgccgcaata tccttccctt cctgatgatg gaaccacggc gctttgattt
    20941 caggataaat accgatattt ttcccggtag agtgatttaa cccctgaaca aattcaatct
    21001 cttcttcaaa ggtgtgcacc cggaagtcgg acttacccat tgggaaacgc cccggataag
    21061 tctgcacttt tttaccgttt tcaatat
//
LOCUS       DAFATA010000080        20851 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFATA010000080 DAFATA010000000
VERSION     DAFATA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.081"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            133..612
                     /gene="coaD"
                     /locus_tag="LGJ20_003423"
     CDS             133..612
                     /gene="coaD"
                     /locus_tag="LGJ20_003423"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK2387440.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(651..1460)
                     /gene="mutM"
                     /locus_tag="LGJ20_003424"
     CDS             complement(651..1460)
                     /gene="mutM"
                     /locus_tag="LGJ20_003424"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBK2387441.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(1558..1725)
                     /gene="rpmG"
                     /locus_tag="LGJ20_003425"
     CDS             complement(1558..1725)
                     /gene="rpmG"
                     /locus_tag="LGJ20_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK2387442.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(1746..1982)
                     /gene="rpmB"
                     /locus_tag="LGJ20_003426"
     CDS             complement(1746..1982)
                     /gene="rpmB"
                     /locus_tag="LGJ20_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK2387443.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(2199..2867)
                     /gene="radC"
                     /locus_tag="LGJ20_003427"
     CDS             complement(2199..2867)
                     /gene="radC"
                     /locus_tag="LGJ20_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK2387444.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            3039..4259
                     /gene="coaBC"
                     /locus_tag="LGJ20_003428"
     CDS             3039..4259
                     /gene="coaBC"
                     /locus_tag="LGJ20_003428"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBK2387445.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            4237..4695
                     /gene="dut"
                     /locus_tag="LGJ20_003429"
     CDS             4237..4695
                     /gene="dut"
                     /locus_tag="LGJ20_003429"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK2387446.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            4802..5398
                     /gene="slmA"
                     /locus_tag="LGJ20_003430"
     CDS             4802..5398
                     /gene="slmA"
                     /locus_tag="LGJ20_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBK2387447.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(5435..6076)
                     /gene="pyrE"
                     /locus_tag="LGJ20_003431"
     CDS             complement(5435..6076)
                     /gene="pyrE"
                     /locus_tag="LGJ20_003431"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBK2387448.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6142..6858)
                     /gene="rph"
                     /locus_tag="LGJ20_003432"
     CDS             complement(6142..6858)
                     /gene="rph"
                     /locus_tag="LGJ20_003432"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK2387449.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            6985..7848
                     /locus_tag="LGJ20_003433"
     CDS             6985..7848
                     /locus_tag="LGJ20_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK2387450.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            8069..8893
                     /gene="dinD"
                     /locus_tag="LGJ20_003434"
     CDS             8069..8893
                     /gene="dinD"
                     /locus_tag="LGJ20_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBK2387451.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            9185..9802
                     /locus_tag="LGJ20_003435"
     CDS             9185..9802
                     /locus_tag="LGJ20_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBK2387452.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(9799..11481)
                     /gene="ligB"
                     /locus_tag="LGJ20_003436"
     CDS             complement(9799..11481)
                     /gene="ligB"
                     /locus_tag="LGJ20_003436"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBK2387453.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            11739..12362
                     /gene="gmk"
                     /locus_tag="LGJ20_003437"
     CDS             11739..12362
                     /gene="gmk"
                     /locus_tag="LGJ20_003437"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK2387454.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            12417..12692
                     /gene="rpoZ"
                     /locus_tag="LGJ20_003438"
     CDS             12417..12692
                     /gene="rpoZ"
                     /locus_tag="LGJ20_003438"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK2387455.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            12711..14819
                     /gene="spoT"
                     /locus_tag="LGJ20_003439"
     CDS             12711..14819
                     /gene="spoT"
                     /locus_tag="LGJ20_003439"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK2387456.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            14826..15515
                     /gene="trmH"
                     /locus_tag="LGJ20_003440"
     CDS             14826..15515
                     /gene="trmH"
                     /locus_tag="LGJ20_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBK2387457.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            15521..17602
                     /gene="recG"
                     /locus_tag="LGJ20_003441"
     CDS             15521..17602
                     /gene="recG"
                     /locus_tag="LGJ20_003441"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK2387458.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(17587..18453)
                     /locus_tag="LGJ20_003442"
     CDS             complement(17587..18453)
                     /locus_tag="LGJ20_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387459.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISVWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(18456..19661)
                     /gene="gltS"
                     /locus_tag="LGJ20_003443"
     CDS             complement(18456..19661)
                     /gene="gltS"
                     /locus_tag="LGJ20_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBK2387460.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            19941..>20851
                     /gene="xanP"
                     /locus_tag="LGJ20_003444"
     CDS             19941..>20851
                     /gene="xanP"
                     /locus_tag="LGJ20_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBK2387461.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPL"
BASE COUNT         5087 a         5679 c         5261 g         4824 t
ORIGIN      
        1 tcaccgacgc cgattaccgt agtttctatg gccgtcatgc cgttgaagta ctgtatcaaa
       61 accagggcgc gctacagcgt ctgcttcaac tgctggaacc ttacctgcca ccgaaaacgc
      121 attgaggttg ttatgcaaaa acgggcgatt tatccgggta ctttcgatcc cattaccaat
      181 ggtcatatcg atatcgtgac gcgcgccacg cagatgttcg atcacgttat tctggcgatt
      241 gccgccagcc ccagtaaaaa accgatgttt accctggaag agcgtgttga gctggcacag
      301 caggcaaccg cgcatctggg gaacgtggaa gtggtcgggt ttagtgattt gatggcgaac
      361 ttcgcccgta atcaacacgc tacggtgctg attcgtggcc tgcgtgcggt ggcggatttt
      421 gaatatgaaa tgcagctggc gcatatgaat cgccacttaa tgccggaact ggaaagtgtg
      481 tttctgatgc cgtcgaaaga gtggtcgttt atctcttcat cgttggtgaa agaggtggcg
      541 cgccatcagg gcgatgtcac ccatttcctg ccggagaatg tccatcaggc gctgatggcg
      601 aagttagcgt agcgtttatg ccggatggta tgccatccgg cgcgcatgaa ttacttctgg
      661 cactgccgac aataaaacgt tgcccgctgc gcatgtttag tcgccacaat cggcgtaccg
      721 cacacccgac acggctcgcc ttttcgcccg taaacctgca attcctgcgc gaaatagccc
      781 ggtttaccat cactttgcag aaaatctttc agcgttgtac caccctgctc aatcgaacgc
      841 agcaacaccg ctttaatcac ccgagctaac aattcacact ctgccagcga cagtgatgac
      901 gccagccgat ccggatggat ccccgccgca aacagtgatt cgctggcata gatattccct
      961 acccctacca ccagcttgtt atccatcagc cacggtttaa tcgccgtttt tttcttcgcg
     1021 cacttctgat gcagatactc accattgaaa tcgtcgctga gcggctccgg tccaagatgg
     1081 gccagcacat tatgcccttc cagctctttg gtccacagcc aggcaccaaa gcggcgcgga
     1141 tcggtgtagc gcagcacttt accgttgctc atcaccaaat ccacatggtc atgcttttca
     1201 gggggaagtt cttctggaag gatgcgcagg ctgccagaca tccccaaatg aatgataatc
     1261 cagccctcag gcagctccag cagcaaatat ttagcccgcc gctgcacgct aagcactggt
     1321 tggtcgctta aacggtagat ctcttctgaa accggccagc gcaagcgtcc gttgcgcacc
     1381 actgcatgaa gaatggttgc accaacgaga tgcggttcta tgccgcgacg gctggtttca
     1441 acttcgggta attcaggcat agcatctcca ggaatgaaca gatgcaatca atatgggggc
     1501 aagcagataa caaaaaaccc cgccggagcg aggttttttg ttacatcaaa gcgagaatta
     1561 tttgattttc gcttctttgt agatcacgtg ctgacgaaca actggatcga attttttcag
     1621 ttccagtttt tccggcttag tacgtttgtt cttcgtagtg gtatagaagt gaccagtacc
     1681 agcagaagaa accagcttga ttttctcacg aataccttta gccatgattt atttcctcta
     1741 agtacttagt acttttcgcc acgggcacgc agttcagcca gaactgtatc gatgcctttt
     1801 ttatcgatta cacgcatacc tttagcagat acgcgcaggg tgacaaaacg cttctcgctc
     1861 tcaacccaga aacggtgaga gtgcaggttc ggcaggaaac ggcgtttagt cgcgttcagt
     1921 gcgtgggaac ggttgttacc ggtcaccgga cgcttgccag taacttggca gactcgggac
     1981 atgtctattc tccaaaaatc aaattagctc gagcttcgta tggggtattg gcgcctcgtc
     2041 aggctttaca gcccggtcat cgcagttcta tgtgaactct cgattgccag gcccaaatgc
     2101 caaacccgag attctcaaag gtggcgtagt atacgctgac tcagcgatgt gctcaagtcc
     2161 cgaacagaca aagatcccga aggatcgcgc atagcgggtt aaatccagcc gcgttcggca
     2221 aaagaaacat actctccacg cccaatcacg atatggtcga gcacgcgtaa atccatgaac
     2281 tgacaactct ttattatccg ttcagtaatg agtttatccg ctttactggg ttcagcacaa
     2341 cccgaagggt gattatgtgc aaggatcagc gccgaggcgt ttatttttat cgcttcgcgg
     2401 ataatttccc gaggatggac ttcaacatgg tttagcgtgc cggaaaaaag tcggctatgc
     2461 gttataaccc ggtgttgtga gtcgagaaag atcaccataa agatctcccg ctcctcaccc
     2521 gtgagctggc tttgtaaaaa ttcccgcgtc atctccgggc tgagtaaagg gctttcttca
     2581 cgcatccgca cgttgtagta acgccgcgcc agttcagcaa tcccctttaa ctgggcaaat
     2641 ttcgccacgc caattccatg aacgccacta aattgttcat attcagaggt taacaagcca
     2701 taaagagagc cgaaattctc cagcatctct tttgccaggg ttaatacatc tttaccgcgc
     2761 gttccggtac gcagaaatag cgccagcagc tcgacatccg ttaaggcgct aataccaaac
     2821 ttcagcattt tttcgcgcgg catcaacagc tgtgaattgt ttttcacctt cacctccttt
     2881 gtggtgctcg catcctgtca tagccgttcc gttaaatcga cggccagttt tcaatcctgg
     2941 aaagcgcctc gcaaagtgat tctcaggtga aggcacgaca acaaaagtat tgtgataaaa
     3001 tcgccaactt ctggtgtcac ccaacaggaa aaatcatcat gagcctggcc ggtaaaaaaa
     3061 tcgttctcgg cgttagcggc ggtattgctg cctataaaac ccctgaactg gtgcgtcgtt
     3121 tgcgcgatcg cggggccgac gtccgcgtag ccatgaccga agcggcaaaa gcctttatca
     3181 ccccacttag cttgcaggcg gtttctggtt atcccgtttc cgacagtctg ctggacccgg
     3241 cagccgaagc cgctatgggc catattgagc tgggtaaatg ggctgattta gtgattctcg
     3301 cccctgccac ggcagatttg attgcccgtg ttgctgccgg aatggcgaat gacctggtat
     3361 cgacgatttg tctggctaca cctgcgcctg tagccgtgct ccccgccatg aaccagcaga
     3421 tgtaccgtgc cgctgccacg cagcataatt tagaggtgct tgcttcccgt ggtttgctca
     3481 tctgggggcc agacagtggc agtcaggctt gtggtgatat cggtcctggg cgaatgctcg
     3541 atccgttaac cattgtggat atggcggtag cgcatttttc gcccgtcaac gacctgaaac
     3601 atctgaacat tatgattacc gccggcccga cgcgtgaacc gctcgatccc gtgcgttata
     3661 tctctaatca cagctccggc aagatgggtt ttgctatcgc cgccgccgct gcccgtcgtg
     3721 gcgcgaacgt cacgctggta tcaggtccgg tttcactacc gacgccaccg tttgttaaac
     3781 gtgttgatgt gatgaccgcg ctggaaatgg aagccgccgt gaatgcttct gtacagcagc
     3841 aaaatatttt tatcggctgc gccgccgtgg cggattatcg cgcagctacc gtggccccag
     3901 agaaaatcaa aaagcaggcc acgcagggtg atgaattaac aataaaaatg gttaaaaacc
     3961 ccgatatcgt cgcaggcgtt gccgcactaa aagaccatcg accctacgtc gttggatttg
     4021 ccgccgaaac aaataatgtg gaagaatacg cccggcaaaa acgtatccgt aaaaaccttg
     4081 atctgatctg cgcgaacgat gtttcccagc caactcaagg atttaacagc gacaacaacg
     4141 cattacacct tttctggcag gacggagata aagtcttacc gcttgagcgc aaagagctcc
     4201 ttggccaatt attactcgac gagatcgtga cccgttatga tgaaaaaaat cgacgttaag
     4261 attctggacc cgcgcgttgg gaaggaattt ccgctcccga cttatgccac ctctggctct
     4321 gccggacttg acctgcgtgc ctgtctcgac gacgccgtag aactggctcc gggtgacact
     4381 acgctggttc cgaccgggct ggcgattcat attgccgatc cttcactggc ggcaatgatg
     4441 ctgccgcgct ccggattggg acataagcac ggtatcgtgc ttggtaacct ggtaggattg
     4501 atcgattctg actatcaggg ccagttgatg atttccgtgt ggaaccgtgg tcaggacagc
     4561 ttcaccattc aacctggcga acgcatcgcc cagatgattt ttgttccggt agtacaggct
     4621 gaatttaatc tggtggaaga tttcgacgcc accgaccgcg gtgaaggcgg ctttggtcac
     4681 tctggtcgtc agtaacacat acgcatccga ataacgtcat aacatagccg caaacatttc
     4741 gtttgcggtc atagcgtggg tgccgcctgg caagtgctta ttttcagggg tattttgtaa
     4801 catggcagaa aaacaaactg cgaaaaggaa ccgtcgcgag gaaatacttc agtctctggc
     4861 gctgatgctg gaatccagcg atggaagcca acgtatcacg acggcaaaac tggccgcctc
     4921 tgtcggcgtt tccgaagcgg cactgtatcg ccacttcccc agtaagaccc gcatgttcga
     4981 tagcctgatt gagtttatcg aagatagcct gattactcgc atcaacctga ttctgaaaga
     5041 tgagaaagac accacagcgc gcctgcgtct gattgtgttg ctgcttctcg gttttggtga
     5101 gcgtaatcct ggcctgaccc gcatcctcac tggtcatgcg ctaatgtttg aacaggatcg
     5161 cctgcaaggg cgtatcaacc agctgttcga gcgtattgaa gcgcagctgc gtcaggtatt
     5221 gcgtgaaaag agaatgcgtg agggtgaagg ttacaccacc gatgaaaccc tgctggcaag
     5281 ccagatcctg gccttctgtg aaggtatgct gtcacgtttt gtccgcagcg aatttaaata
     5341 ccgcccgacg gatgattttg acgcccgctg gccgctaatt gccgcgcagt tgcagtaata
     5401 tgacgccgga tgacttttca tccggcgagt ttctttaaac gccaaactct tcgcgatagg
     5461 ccttaaccgc cgccagatgt tccgccattt ccggcttctc ttccagatag gcaatcagat
     5521 ctttcagggt gatgatagag atcactttgc agttgtaatc acgctcaact tcctgaatcg
     5581 ccgaaatctc gccgcgcccc cgttcctgac gatcaagcga aatcaatacg ccagcaagcg
     5641 tcgcgccatt agcctgaata atctccatcg attcgcgaat cgccgttccg gcggtgatca
     5701 catcatctac cagcattacg cgtccttgta acgcgctacc aaccagattg ccgccttcac
     5761 cgtggtcttt tgcctctttg cggttaaagc agtacggcag gtcaaggtca tgatgctccg
     5821 ccagcgccac ggcggtagtg gtcgcaatcg ggatcccttt gtaagcaggg ccaaacagca
     5881 gatcgaactc aaggccggaa tccaccaacg cttcagcgta aaaacggcct aacagtgcca
     5941 gatcgcgccc ggtattaaac agcccggcgt tgaagaaata ggggcttttg cgcccggact
     6001 tcaacgtaaa ctcgccaaac tttaacacct gcttgccaag cgcaaattca ataaactggc
     6061 gctgatatgg tttcatgcct tcgctcctca tcttactttt ctacagacaa aaaaaaggcg
     6121 actcatcagt cgccttaaaa atcagtttgc cagcgccgcc ttctgcgtcg ctacaatgga
     6181 ttcgattccc cctcgggcca gagccaacaa ggtgagtagc tcttcatggg tgaacggctc
     6241 gccttctgcc gtcccctgca cttcaatgat gcgcccgtct tcggtcatca ctacgttcat
     6301 gtcggtctct gcggcagagt cttcaacgta ttccagatcg caaaccgctt cgccgttcac
     6361 aattccgaca gaaactgcgg ctaccatccc tttcatcgga ttggttttca gcttgccgtt
     6421 ttccaccagc ttctgtagcg catctgccag cgccacgcag gcacccgtaa tcgacgcggt
     6481 acgcgtgcca ccatcagcct gaagcacgtc gcagtccagc gtaatggtga actcacccag
     6541 cgctttcaaa tctactgccg cgcgaagagc acgggcgatc agacgctgga tttccattgt
     6601 gcgtccaccc tgcttacctt tcgccgcttc acgagcgtta cgggtgtggg tagaacgtgg
     6661 cagcatgccg tactctgcgg tgatccagcc ctggccctga cctttcagga agcgcggcac
     6721 gccttcttca atagaggcgg tacacaacac tttggtatcg ccaaattcga ccagcaccga
     6781 gccttctgca tgttttgtat agttacgagt cagggtaacg ggacgcacct gattattgct
     6841 acggcctgct ggacgcatat tgaaatctcc ggcttgaaac aaatgtggct gcgcattata
     6901 cggacttccg gcggttattc ctatcctgac aaggcatcga tggctataat ccttccacct
     6961 ctccttttat aaacaggaac gtctatgatc cgcagtatga ccgcctacgc ccggcgtgaa
     7021 atcaagggtg aatgggggag cgcaacctgg gaaatgcgct cggtaaacca gcgttatctg
     7081 gaaacttact ttcgtctgcc ggagcagttc cgtagccttg aacctgtcgt tcgcgagcgt
     7141 attcgttctc gcctgacgcg cggtaaagtg gaatgtaccc tgcgctatga gccagatgtt
     7201 agcgcgcaag gtgagctgat cctcaacgaa aaactggcta aacagctggt aactgccgcg
     7261 aactgggtaa aaatgcagag tgacgaaggg gaaatcaacc cggttgatat tctacgctgg
     7321 ccgggcgtga tggcagccca ggagcaggat cttgacgcca ttgccgctga aattctcgcg
     7381 gcgctggatg gtacgctgga cgactttatt gtcgcgcgcg aaaccgaagg tcaggcactg
     7441 aaagcattga tcgagcagcg tctggaaggc gtcaccgccg aagtggtcaa agtccgcgcc
     7501 catatgccgg aaatcctgca atggcagcgt gagcgtctgg tcgcgaagct ggaagatgct
     7561 caggttcagt tggaaaacaa ccgtctggag caggagctgg ttctgctggc acaacgaatt
     7621 gacgttgccg aagaactgga ccgcctggaa gcgcatgtca aagagaccta caacattctg
     7681 aagaaaaaag aagccgttgg tcgtcgtctg gactttatga tgcaggaatt caaccgcgag
     7741 tcgaacactc ttgcgtcgaa gtctatcaat gccgaagtga caaactccgc gattgagctg
     7801 aaagtgttga ttgagcagat gcgcgagcag attcagaaca ttgaataact cgtaacgcca
     7861 attcttactt ttcagccttc acaaatgccg cccactcaaa cagagcggca tttttcttcc
     7921 ccgcaacttc aattctgttt tgcgtgcctg ctccagattt tgcgatgttt ttttgcccag
     7981 cacactgaga acgtgagata ctcacaactg tatataaata cagttacaga tttactttct
     8041 ttgcaatcga tatcacatgg agtgggcaat gaacgaacat catcaacctt ttgaagaaat
     8101 acggcattat ggcactgaag ggcaagaatt ttggtcagct agagaattgg caccgttact
     8161 ggattatcgg gactggcgca attttcaaaa ggtactggcc cgcgcgactc aagcgtgcga
     8221 agccagcaat caagctgcat ctgaccattt cgttgaaacc accaaaatgg ttgtattagg
     8281 ttcaggggct caacgagaac ttgaagatgt tcatctctcc cgctatgcct gttacctggt
     8341 agtacaaaac ggcgaccctg cgaaaccggt tattgcggca gggcaaactt attttgctat
     8401 ccagacccga cggcaggagc tggctgacga tgaagctttc aggcaacttc gtgaagacga
     8461 aaagcgtctt tttctgagaa atgaattgaa agaacataac aaacaattgg ttgaggcagc
     8521 gcagcaagct ggggtagcga cagctatcga tttcgccata tttcagaatc atggttaccg
     8581 ggggctgtat ggtggattag atcagaaagc catccatcag cggaaggggc tgaaaaagag
     8641 tcagaagatc ctggatcata tgggttcaac agaactggcg gctaatctct ttcgagctac
     8701 ccaaacagaa gaaaaactca agcgggatgg cgtgaattca aaacagcaag ctaataccac
     8761 acactttgac gtgggtagca aggtgaggca aaccattcag gaacttggcg gaaccatgcc
     8821 tgaggagttg ccgaccccgc aggtcagcat caagcagttg gaaaacagtg taaaaattac
     8881 agagaagaaa tagcagaaac taaaaactct ttttgttgat tgagataccc gatgcgtaag
     8941 ccaaagtcca ggtgcggtga acacaatggc taaacattgc acctttcttt tccacccctc
     9001 aattttaacc tattttttct tatgcatttt ctcagacaag aagtcagaag aatgcatcta
     9061 cgctacagaa aatagcgatt tcacataact acaagttatc aatttcctct caccttaaaa
     9121 aatctcaatc gtgacaatac gcacaaatcg ctaccctgcc agacagattt ttagggagag
     9181 aaccatgctg ttacacattt tgtatttggt tggcattact gccgaagcca tgacgggggc
     9241 tctggcggcc gggcgacgcc gcatggatac atttggcgta attattattg cgaccgccac
     9301 cgcaattggc ggagggtcag tgcgcgatat tctgctgggc cactatccgc tcggctgggt
     9361 caaacacccg gaatatgtga ttatcgtcgc taccgccgca gtactcacca ctatcgttgc
     9421 tcctgtaatg ccttacctgc gcaaagtgtt tctggtgctc gatgcgcttg gactggtggt
     9481 cttttctatc atcggcgcac aggttgcact ggatatgggg cacggcccaa ttattgccgt
     9541 tgtcgcggcg gtaaccaccg gcgtgtttgg cggcgttttg cgcgatatgt tctgtaaacg
     9601 tatcccactg gtattccaga aagagttgta tgccggtgtc tcatttgcct ccgccgtgct
     9661 gtacatcgcg ctgcaacact atgtttctaa ccatgatgtg gtaattatct ccaccctggt
     9721 attcggcttt tttgcccgct tactggcgct acgccttaag ctgggattac cggtctttta
     9781 ctacagccac gaaggacact aaggttcaaa acctgtgatc tgctgggcag ccagccaact
     9841 gcccagcttc ttgatttgcg cattttcctt ccattcaata acctgtctgg cgcgccccga
     9901 tccagtcccc ggcagctgct gccagaactg ctccgtgcta aataaaagtt gcgaccagga
     9961 ccgttcatca ctggcattaa gcgccgcccg ggttagcggt attcccattg ccatcaccca
    10021 gcgagtaaaa ggctgcttac gagccagatt aaaccgatgc catagctgcg cacttttact
    10081 tttcgcgatc cccggcgtgt tctgtaattg ctctggcgtt aataaaagcc aggaaaagat
    10141 atgctcaaag cgatgagtct gatgcagcgc gcgccagccg gcctcaccaa taccatccag
    10201 ccccagaacc tgttttgacc ccagccagac taagcgtgaa atgaactgtt cctgacaaac
    10261 atcagaagca aagtagcagg tcaacgagtt aaagcggttt tctggtggtg tcggttttgt
    10321 acgttctgca ccgcgccaga ccacatcatc aatgcgagga ataccctgac cggcaaggct
    10381 gacgaggatc tgatcaccag gcgcaatatc ccactcctgc cagcgcctga cggaaccaat
    10441 attcacccgc tggacttttt tatcatccag catgacaggt gcgagtgacg caaccaccga
    10501 tattttaccg ctcttaccca ccgcaaactg aatcgccttc acttcggcaa cctgagctac
    10561 aggttgatat ttccaggcca ccagccattc tgcctggcct ggtagccaat ggcgggattc
    10621 gggttctttc gcccctcgca caaccacgcc atcggtgacg aagggtaatt tcgctttcca
    10681 ccactcattg cgtacgcgcg caacctcatc agcattttta accgcacggg tatacctctg
    10741 cgttagagta aaacctgcgg tagccagctc ctttaaacga tcggtcatta actgcggtcc
    10801 atccggccat gcccagacaa aaacgcccag agaattcagc gtgtcgctat cgtcctggcg
    10861 catcatcagg ccagcaactt ttgcgcgggc atttattccc cccatttgtt gttggatatg
    10921 cccctcgcgc tggagaaata tttccccctg aagcgtactg ttggctaaag gcccgctaac
    10981 ggtttgcggc acagcggaaa ttaagctaac tttctgcgtc cagtcctcgc ctttcaggcc
    11041 attaccgcga ctgattgctt tgttcagttt cccgtcccga taaaccaggg ttaccgcaac
    11101 gccatcaact tttggctgca cccaaagatc gctacgttct cgcatccaca gacttaatgc
    11161 gtttttatcc accattttgc gtacacccgt atgggcaacc ggatgcataa ccgcaccatt
    11221 taaaggcggc atcatcacgt cgcggggctc gctcccaaag cagcgttgcc actgcgtaag
    11281 acgggcgctt aactgatcgt aaacaccgtc ttccacctca ctttttcctt ccttccagta
    11341 gtcatcgtcc cactgtttta tttgctgttg caggcgggaa atttcttcct gtgctctggc
    11401 tggcgaccag gccggacaga ccgcccacac agatgattgc cagcacaaga tacttattaa
    11461 tatcgccatc catactttca tcatcacctc cgctgtaggt agtcaggcag atatacaacg
    11521 tgatgaaagc aaagccgagt ggcaaaaacg gagtctgcga ggacgcttcc tgaaaatcgt
    11581 ctttattgca gtgaatgaca ggcaaatgcg gaagcagcta cgcaaaacgc aacaactttg
    11641 cgcaaaaagt gtgagcaagg gctacgtcac atggccgcgc cgtgtataat aagctcgtat
    11701 gtaggcttta tttcgctaat cacatacgaa agatactcat ggctcaaggc acgctttata
    11761 ttgtttctgc ccccagtggc gcgggtaaat ccagcctgat tcaggcttta ttaaaaaccc
    11821 aaccgttgta tgacacccag gtttctgttt cacacaccac acgccaaccg cgtcctggtg
    11881 aagtccacgg tgaacattat ttctttgtta atcatgatga atttaaagaa atgattagca
    11941 gagatgcgtt cctcgaacac gcagaagttt ttggtaatta ctatggcact tcgcgtgagg
    12001 ccattgagca agtactggcg accggtgtcg atgtttttct cgatatcgac tggcagggcg
    12061 cgcagcaaat tcgccagaaa atgccgcacg cgcggagtat ctttatttta ccgccgtcca
    12121 aaattgaact ggaccgccgt ctacgcggtc gcggtcagga cagcgaagag gtcattgcaa
    12181 agcgtatggc gcaagctgtt gcagaaatga gccattacgc cgaatatgat tatctgattg
    12241 tgaatgatga cttcgatacc gcgttgaccg atttgaagac cattattcgc gccgaacgtc
    12301 tgcgcatgag ccgccaaaag cagcgtcatg acgctttaat cagcaaattg ttggcagact
    12361 gaacctgatt tcagtatcat gcccagtcat ttcttcacct gtggagcttt ttaagtatgg
    12421 cacgcgtaac tgttcaggac gctgtagaga aaattggtaa ccgttttgac ctggtactgg
    12481 tcgccgcgcg tcgcgctcgt cagatgcagg taggcggaaa ggatccgctg gtaccggaag
    12541 aaaacgataa aaccactgta atcgcgctgc gcgaaatcga agaaggtctg atcaacaacc
    12601 agatcctcga cgttcgcgaa cgccaggaac agcaagagca ggaagccgct gaattacaag
    12661 ccgttaccgc tattgctgaa ggtcgtcgtt aatcacaaag cgggtcgccc ttgtatctgt
    12721 ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc aagcgtctgc
    12781 ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca agcggtgaac
    12841 cctatatcac gcacccggta gcggttgcct gcattctggc cgagatgaaa ctcgactatg
    12901 aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc acctaccagg
    12961 acatggaaca gctttttggt aaaagcgtcg ccgagctggt agagggggtg tcgaaacttg
    13021 ataagctcaa gttccgcgat aagaaagagg cgcaggccga aaactttcgc aagatgatta
    13081 tggcgatggt gcaggatatc cgcgtcattc tcatcaaact tgccgaccgt acccacaaca
    13141 tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt gaaactctcg
    13201 aaatttatag cccgttggcg caccgtttag gtatccacca cattaaaacc gaactcgaag
    13261 agctgggttt tgaggcgctg tatcccaatc gttaccgcgt aattaaagaa gtggtgaaag
    13321 ccgcgcgcgg caaccgtaaa gagatgatcc aaaaaatcct ctctgaaatc gaagggcgtt
    13381 tgcaggaagc gggaataccg tgccgcgtca gtggtcgcga aaagcatctt tattcgattt
    13441 actgcaaaat ggtgctcaaa gagcagcgtt ttcactcaat catggacatc tacgctttcc
    13501 gcgtgatcgt caatgattct gacacctgtt atcgcgtgct cgggcaaatg catagcctgt
    13561 acaagccgcg tccgggtcgc gtgaaagact atatcgccat tcctaaagcg aacggctatc
    13621 agtcgttgca cacctcgatg attggcccgc acggcgtgcc ggttgaggtc cagatccgta
    13681 ccgaagatat ggatcagatg gcggagatgg gtgttgccgc gcactgggct tataaagagc
    13741 acggcgaaac cagtactacc gcacaaatcc gcgcccagcg ctggatgcaa agcctgctgg
    13801 agctgcaaca gagcgccggt agttcgtttg aatttatcga gagcgttaaa tccgatctct
    13861 tcccggatga gatttacgtt ttcacaccgg aagggcgaat tgtcgagctg cctgccggtg
    13921 caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc tgcgtgggcg
    13981 cacgcgttga ccgccagcct tacccgctgt cgcagccgct taccagcggt caaaccgttg
    14041 aaatcattac cgcaccgggc gctcgcccga atgccgcgtg gctgaacttt gtcgttagct
    14101 caaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat gattctgtaa
    14161 gcctgggccg tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg aatgaaatcc
    14221 cgcaggaaaa tattcagcgc gagctggatc gcatgaagct ggcaacgctt gacgatctgc
    14281 tggcagaaat cggccttggt aacgcaatga gcgtggtggt cgcgaaaaat ctacaacatg
    14341 gggacgcctc cattccaccg gcgactcaaa gccacgggca tctgccgatc aaaggcgctg
    14401 atggcgtgct gatcaccttt gcgaaatgct gccgccccat tcctggcgac ccgattatcg
    14461 cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt aacatccgtg
    14521 gctaccagaa agagccagag aagtttatgg ctgtggaatg ggataaagag acggcgcagg
    14581 agttcatcac cgaaatcaag gtggagatgt tcaatcatca gggtgcgctg gcaaacctga
    14641 cggcggcaat taacaccacg acctcgaata ttcaaagttt gaatacggaa gagaaagatg
    14701 gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga ccgtgtgcat ctggcgaata
    14761 tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac cgaaattaat
    14821 gttttatgaa cccaacacgt tatgcacgca tctgcgaaat gctcgccagg cggcagcctg
    14881 atctgaccgt ctgcatggag caggtccaca aacctcataa cgtttctgcg attattcgta
    14941 ccgcagatgc cgttggcgta catgaagttc acgccgtctg gcctggtagc cgcatgcgca
    15001 ccatggcttc ggcagcggcg ggtagtaaca gctgggtaca ggtgaaaaca caccgcacca
    15061 ttggcgatgc cgtcgctcat ctcaaaggcc agggaatgca gattctggca acccatcttt
    15121 ctgataacgc tgtcgatttc cgcgaaattg attacactcg cccgacctgc attttgatgg
    15181 gacaggagaa aacgggcatc acgcaggaag cattggccct ggcggatcag gacatcatca
    15241 ttccgatgat cggcatggtg cagtcgctga atgtttccgt tgcctcagcc ctcattcttt
    15301 acgaagccca gcgtcagcgg caaaatgcag gcatgtacct gcgtgaaaac agcatgttgc
    15361 cggaagcaga gcaacaacgc ctgttgtttg aaggcggcta tccggtgctg gcgaaagtcg
    15421 caaaacgcaa aggcctgcct tatccccacg tcaatcagca aggcgagatc gaagctgatg
    15481 ccgactggtg ggctactatg caggctgcag ggtaagtgcc atgaaaggtc gcctgttaga
    15541 tgctgtccca ctcagttccc taacgggcgt tggcgcagca cttagtaaca aactggcgaa
    15601 aatcaacctg cataccgtgc aggatctact cttacacctt cccctgcgct acgaagatcg
    15661 cacccatctc taccccatcg gagaactact gccgggcgtt tatgccacgg tggaaggcga
    15721 agtgctgaac tgcaatatct ccttcggcgg tcggcggatg atgacctgcc agatcagcga
    15781 cggttccggc atcctcacca tgcgcttttt caatttcagc gcggcaatga aaaatagcct
    15841 ggcgacgggc cgccgtgtac tggcttatgg cgaagcaaag cgcggtaaat atggtgcgga
    15901 gatgatccac ccggaatacc gcgtgcaggg cgatctcagc acgccagaat tacaggaaac
    15961 gctcacgccg gtttatccaa caacggaagg cgtaaagcag gccacgctgc gtaaattaac
    16021 cgaccaggcg ctggatctgc tcgacacctg cgccattgaa gaactcctgc cgccggaact
    16081 gtcacaagga atgatgacgc taccggaagc gttgcgcact ttgcaccgcc cgccaccgac
    16141 gctacagctt agcgatctgg aaaccgggca gcatccggcg caacgtcgtc tgattctgga
    16201 agaactgctg gcgcacaacc tcagcatgtt agccttacgt gccggagcac agcgttttca
    16261 tgcccagccg ctgagcgcca atgacacgct gaaaaataaa ctcctcgccg ccttaccgtt
    16321 caagccaacg ggcgcacagg cacgcgtagt ggcggagatc gagcgcgata tggcgctgga
    16381 tgtgccgatg atgcgtctgg tgcagggcga tgtaggttcc ggtaaaacgc tggtcgccgc
    16441 cctcgccgcg ttgcgtgcga ttgcccacgg caaacaggta gcattgatgg caccaaccga
    16501 attactcgcc gagcagcacg ccaataactt ccgcaactgg tttgcaccgc tcggtatcga
    16561 agtgggctgg ctcgccggta agcagaaagg taaagcacgg ctggcacagc aggaagccat
    16621 cgccagcggt caggtgcaga tgattgtcgg tacacacgcc atcttccagg aacaggtgca
    16681 gtttaacggc ctggcgctgg tgattatcga cgaacagcat cgttttggcg tgcatcagcg
    16741 tctggcattg tgggagaaag gccagcagca gggcttccat ccgcatcagt tgatcatgac
    16801 cgccacgccg atcccccgca cgctggcaat gactgcgtat gccgatctcg atacctcggt
    16861 gatagatgag ctgccgccag gccgcacgcc agtgactacg gtcgctattc ctgatacccg
    16921 ccgtaccgac atcattgacc gcgtgcgcca cgcctgcata actgaaggtc gtcaggcata
    16981 ctgggtttgt acgttgattg aagagtcgga attgctggaa gcgcaggcgg cggaagctac
    17041 ctgggaagag ttgaaactgg cgctaccaga gttgaacgtt ggcctggtac acgggcggat
    17101 gaaacctgtc gagaaacagg cggtgatggc gtcgtttaaa caaggtgagc tacacctgct
    17161 ggttgccaca accgttattg aagtcggcgt tgatgtgcct aacgccagtc tgatgattat
    17221 cgaaaacccg gagcgtctgg gtctggcgca gttacaccag cttcgcgggc gtgtaggtcg
    17281 tggtgcggtg gcttctcact gcgtgctgct ctacaaaacg ccgctctcca aaaccgcgca
    17341 aattcggctg caagtgctgc gcgacagcaa cgacggtttt gtgattgcac agaaagactt
    17401 agagattcgc ggaccgggtg aactgttagg tacgcgtcag acgggtaatg ctgagtttaa
    17461 agtggcggat ttactgcgcg atcaggcgat gatcccggaa gttcagcgcc tggcgcgaca
    17521 tattcacgaa cgttacccac aacaagcaaa agccctgata gaacgctgga tgccggagac
    17581 ggaacgttac tccaacgcat gaccagactc atcacaagac aagtcacgca gtaaatcttc
    17641 aatctggata gtgaggatat ccattgcctc atacgcatcc tgtgcaattc gcgccgccat
    17701 tgcgccttcc ggttcaccac gcagaatttc agccgctgcc tgtacgccag ccacaatcat
    17761 ttcaggtgtt aacatttggc gaagaaaagc gcgattatca gaggaccagc catctttatg
    17821 gcctaatgga tgcccccagc ctatccattc ttgcgaccat aacggattgg cttctatggg
    17881 ataaattaga tgcgcggcat actgaatagc acacaccgct tttcctggcg tatccagcgc
    17941 ccaccagcgc gaccagagag aatgaattaa cggcgcggcc ccacccagag tattgaacac
    18001 cgccagccag caaagaacgt tattgaaacc acgctcgtta tccatacgct gcaacgcggt
    18061 gtccagtaaa aattgccgta cctgctggcg ttgggaagca ttcatcatct cctgccagag
    18121 atagggccga cacaatgcct tcattaagga ttcttcgaag tgatcaccat aaccgccagc
    18181 taaaacggtt tcatgccatt gcgccaggca gagcggaaac agatagcgaa acaactctat
    18241 ttgcatcggt tcgttataaa cgagattgag tacatagtaa gcactcaaat cccatacgct
    18301 gatctgctgc cactctttag ccgccagcgc cttgagttcg gcatctgcgt aatcaaattc
    18361 cgtttcccat acacagagca ctggcggctc atcaccaaaa atatggcgac agtgttcacg
    18421 tagcgtttcc ggttccttcc gacgcttttt catttttaac cggcaaaaat cggcaacatt
    18481 aaatacagct taatcaccag cgcattgacg atatcgataa agaacgcacc gaccatcggc
    18541 accaccaaaa acgccatgtg cgacgggcca aagcgttcag tgatcgcctg catgttggcg
    18601 attgccgttg gcgttgcacc gaggccaaaa ccacagtgac ccgcagccag cactgccgca
    18661 tcgtagtttt tgcccatcat gcgccaggta acgaagatgg catacaacgc catgaagatg
    18721 gtctgtacca ccagaatcgc cagcatcggc agcgccagcg aagccagctc ccacagtttc
    18781 agccccatca gcgccatcgc caggaacaac gacaggctta cgttacccag cacggatacc
    18841 gcacgctcaa agacgcggta aaagcccatc attgacagac cgttgctcag aatcacgcca
    18901 acaaacagta cacagacgaa ggtcggcagt tcaaaagcag tgccagccaa aagttgcgca
    18961 acaattttcc ccaccgtcag gcagatagca atcagcgcga tagtttcaat cagcaccagc
    19021 gaggtgatca tgcggcccac atccggcttt tcaaacgccg tcgggacttc ctggtcatcc
    19081 ggaataccgt tcggcgtggt ggagtgtttc accagatagc gcgccaccgg accgccaatc
    19141 aagccgccca gcaccagacc gaacgttgca caggccatcg ccacttccgt cgcattggtg
    19201 aagccataac gttcaatgaa caatttactc cacgcagcgc ccgtaccgtg accgccggaa
    19261 agcgtaatag aaccggccaa cagccccatc agcggatcaa gccctaacag gctagccata
    19321 ccaatgccaa tggcattttg catcaccaac agaccaacaa ccacaatcaa gaagatgcca
    19381 accacacgcc caccggcacg caaactggca atgttggcgt tcaggccaat ggtggcgaag
    19441 aaagccagca ttaacggatc gcgcagggac atatcaaagt tgacttccca gcccatgctt
    19501 tttttcagta ctagtagcgc cagcgccacc aacaaaccac ccgcaacagg ttccggtatg
    19561 gtgtatttct tcaaaaagga gacggaatgg accaacttac gcccgagcag caacgtcagc
    19621 gttgcggcaa caagcgttgc taaagtatcg agatgaaaca tagttactcc tttgtatccg
    19681 catcactctt tcatactcgt catacttcat cgcgcagtgg caacttcatg aactatgacg
    19741 ggtatcgagt tatgctttcc tgggtcaaat tctcagctgt taatgaacaa atggcatgga
    19801 ttttaagcag aaaaatacca aaagttatat aaaaaagtgc atttatctca tttaaatatt
    19861 tagatgagga tgtggcaaac gtttgctttt ctctttcggt cagctaaaat gcccgctttg
    19921 ctaccacggg attgttttcg atgtctgttt ccaccctcga gtcagaaaat gcgcaaccgg
    19981 ttgcgcagac tcaaaacagc gaactgattt accgtcttga agatcgtccg ccgcttcctc
    20041 aaaccctgtt tgccgcctgt cagcatctgc tggcgatgtt cgttgcggtg atcacgccag
    20101 cgctattaat ctgccaggcg ctgggtttac cggcacaaga cacgcaacac attattagta
    20161 tgtcgctgtt tgcctccggt gtggcatcga ttattcaaat taaggcctgg ggtccggttg
    20221 gctccgggct gttgtctatt cagggcacca gcttcaactt tgttgccccg ctgattatgg
    20281 gcggtaccgc gctgaaaacc ggtggtgctg atgttcctac catgatggcg gctttgttcg
    20341 gcacgttgat gctggcaagt tgcaccgaga tggtgatctc ccgcgttctg catctggcgc
    20401 gccgcattat tacgccgctg gtttctggcg ttgtggtgat gattatcggc ctgtcgctaa
    20461 ttcaggttgg gttaacgtcc attggcggcg gttacgcagc catgagcgat aacaccttcg
    20521 gcgcaccgaa aaatctgctg ctggcaggcg tggtcttagc cttaattatc ctgcttaacc
    20581 gtcaacgtaa cccttactta cgcgtggcct cactggtgat tgcgatggcg gccggatatg
    20641 cgctggcgtg gtttatgggc atgttgccag aaagcaacga accgatgacg caagaactga
    20701 ttatggtgcc aacgccgctc tattacggtc ttggcattga atggagtctg ctgctgccgc
    20761 tgatgctggt ctttatgatc acttcgctgg aaaccattgg cgatatcacg gcgacctctg
    20821 acgtttccga acagccagtg tccggtccgc t
//
LOCUS       DAFATA010000081        20303 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFATA010000081 DAFATA010000000
VERSION     DAFATA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.082"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..456
                     /locus_tag="LGJ20_003445"
     CDS             <1..456
                     /locus_tag="LGJ20_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBK2387462.1"
                     /translation="RTVPSQMYYTPQWFKRMERWLGDSSLQPLVNLVQHHETSYRDIQ
                     QFIEKPPGKLRIFEIYPPKPLHSIALGSRIPALREDYKLGRLCGRYFLATVGKLLTEK
                     APLTRHLVPVVTPESIVIPPAPVANDTLVAEVSDAPQANDPTFNNEDLA"
     gene            453..1235
                     /locus_tag="LGJ20_003446"
     CDS             453..1235
                     /locus_tag="LGJ20_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2387463.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(1348..2211)
                     /locus_tag="LGJ20_003447"
     CDS             complement(1348..2211)
                     /locus_tag="LGJ20_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBK2387464.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(2183..3733)
                     /locus_tag="LGJ20_003448"
     CDS             complement(2183..3733)
                     /locus_tag="LGJ20_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBK2387465.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            3991..4770
                     /gene="deoC"
                     /locus_tag="LGJ20_003449"
     CDS             3991..4770
                     /gene="deoC"
                     /locus_tag="LGJ20_003449"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK2387466.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            4897..6219
                     /gene="deoA"
                     /locus_tag="LGJ20_003450"
     CDS             4897..6219
                     /gene="deoA"
                     /locus_tag="LGJ20_003450"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBK2387467.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            6271..7494
                     /gene="deoB"
                     /locus_tag="LGJ20_003451"
     CDS             6271..7494
                     /gene="deoB"
                     /locus_tag="LGJ20_003451"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK2387468.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            7574..8293
                     /gene="deoD"
                     /locus_tag="LGJ20_003452"
     CDS             7574..8293
                     /gene="deoD"
                     /locus_tag="LGJ20_003452"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK2387469.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(8568..8717)
                     /locus_tag="LGJ20_003453"
     CDS             complement(8568..8717)
                     /locus_tag="LGJ20_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387470.1"
                     /translation="MIDLENQEREIINIMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(8749..9765)
                     /gene="lplA"
                     /locus_tag="LGJ20_003454"
     CDS             complement(8749..9765)
                     /gene="lplA"
                     /locus_tag="LGJ20_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBK2387471.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(9793..10437)
                     /locus_tag="LGJ20_003455"
     CDS             complement(9793..10437)
                     /locus_tag="LGJ20_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBK2387472.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            10543..11511
                     /gene="serB"
                     /locus_tag="LGJ20_003456"
     CDS             10543..11511
                     /gene="serB"
                     /locus_tag="LGJ20_003456"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBK2387473.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            11560..12942
                     /gene="radA"
                     /locus_tag="LGJ20_003457"
     CDS             11560..12942
                     /gene="radA"
                     /locus_tag="LGJ20_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK2387474.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            12963..14195
                     /gene="nadR"
                     /locus_tag="LGJ20_003458"
     CDS             12963..14195
                     /gene="nadR"
                     /locus_tag="LGJ20_003458"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBK2387475.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLVIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(14502..16169)
                     /gene="ettA"
                     /locus_tag="LGJ20_003459"
     CDS             complement(14502..16169)
                     /gene="ettA"
                     /locus_tag="LGJ20_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBK2387476.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            16380..18317
                     /gene="sltY"
                     /locus_tag="LGJ20_003460"
     CDS             16380..18317
                     /gene="sltY"
                     /locus_tag="LGJ20_003460"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBK2387477.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18407..18733
                     /gene="trpR"
                     /locus_tag="LGJ20_003461"
     CDS             18407..18733
                     /gene="trpR"
                     /locus_tag="LGJ20_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBK2387478.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18775..19287)
                     /gene="yjjX"
                     /locus_tag="LGJ20_003462"
     CDS             complement(18775..19287)
                     /gene="yjjX"
                     /locus_tag="LGJ20_003462"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBK2387479.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            19339..19986
                     /gene="gpmB"
                     /locus_tag="LGJ20_003463"
     CDS             19339..19986
                     /gene="gpmB"
                     /locus_tag="LGJ20_003463"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBK2387480.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(19983..>20303)
                     /locus_tag="LGJ20_003464"
     CDS             complement(19983..>20303)
                     /locus_tag="LGJ20_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK2387481.1"
                     /translation="TRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMF
                     TYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRPINLRCELLI
                     PIRR"
BASE COUNT         4689 a         5297 c         5637 g         4680 t
ORIGIN      
        1 cgcactgtgc cgtcacaaat gtactacacg ccgcagtggt tcaaacgcat ggaacgctgg
       61 ctgggtgaca gtagcctgca gccgctggtc aatctggtgc agcatcatga aaccagctat
      121 cgtgacattc agcaatttat tgagaaacca cctggcaagc tgcggatatt cgaaatttat
      181 ccaccgaagc cattacatag tatcgcgctt ggcagtcgga ttccggcgct gcgtgaagac
      241 tataaacttg ggcgtttatg cggtcgttat ttcctcgcca cggttggcaa gctattaact
      301 gaaaaagcgc cgcttacccg ccatctggtg ccagtggtga cgccggaatc gatcgttatt
      361 ccgcctgcgc cagtcgccaa cgatacgctg gttgccgaag tgagcgatgc tccgcaggcg
      421 aacgacccga catttaacaa tgaggatctg gcttgatttg ccgttttatc gatacccact
      481 gccattttga tttcccgccg tttagtggcg atgaagaggc cagcctgcaa cgcgcggcac
      541 aagcgggcgt aggcaagatc attgttccgg cgacagaggc ggaaaatttt gcccgcgtgc
      601 aggcgttagc ggaaaaatat caaccgctgt atgccgcatt ggggttgcat ccggggatgt
      661 tggaaaaaca tagcgatgtg tctcttgatc agctacagca ggcgctggaa aggcgtccgg
      721 cgaaggtggt ggcggtgggg gagatcggtc tggatctctt cggcgacgat ccgcaatttg
      781 agaggcaaca gtggttactc gacgaacagc tgaaactggc gaaacgctac gatctgccgg
      841 tgatcctgca ttcacggcgc acacacgaca aactggcgat gcatcttaaa cgccacgatt
      901 tatcgcgcac tggcgtggtt cacggttttt ccggcagcct gcaacaggcc gagcggtttg
      961 tacagctggg ctacaaaatc ggcgtaggcg gtactatcac ctatccacgc gccagtaaaa
     1021 cccgcgatgt catcgcaaaa ttaccgctgg catcgttgtt gctggaaacc gacgcgccgg
     1081 atatgccgct caacggcttt caggggaagc ctaaccgccc ggaacaggct gcgcgggtgt
     1141 ttgacgtgct ttgcgagtta cgtccggaac cagaagatga gattgccgaa gtgttgctta
     1201 ataatacgta tgcggtgttt agcgttcgtg ggtagtttac ccttcgtagg cagcatccgg
     1261 cagtcggcgc ataatgcctg atgcgacgct tgtcgcgtct tatcatgcct acaggtttgt
     1321 gccgaaccgt aggccgtatc cggcatgtca caaatagagc gccggaaata tcaaccggct
     1381 caccccgcgc acctttaacg catcagccaa cggctcaacg tcttccggcg tggcgctcgc
     1441 ccagctttgc gcctcgccat acacgccgtg ggcatgaaac gcattcaggc gaaccggaac
     1501 atcgccaagt ctcttgataa acgtcgccag ctcatcgatg tgatgcaaat aatccacctg
     1561 gtctggaatt accagcaaac gcagttccgc cagcttaccg cgctctgcca gcaaacagat
     1621 gctgcgctta atctgctgat tatcgcgtcc ggttaattgc tgatgacatt cgctccccca
     1681 cgctttgaga tcgagcatcg cgccgtcgca caccgggagt aatttttccc agccggtttc
     1741 gctcaacata ccgttactgt ccacgaggca ggtgaggtgg cgcagttgcg gatcgttttt
     1801 gatggctgta aatagcgcca ctacaaacgg cagttgggtc gtggcttcgc cgccgcttac
     1861 cgttatccct tcaataaaca gcaccgcttt gcggacatgg ctaagcactt cgtccacgct
     1921 catggattgc gccattggcg tggcatgttg cggacacatc ttcaggcagg tatcacactg
     1981 ctcgcagacc acagcgttcc acagcacttt gccgtcaaca atctgcaacg cctgatgcgg
     2041 acactgtggc acgcactctc cgcaatcatt gcagcgtccc atcgtccacg gattgtgaca
     2101 gtttttgcag cgcagattgc agccctgcaa aaacagagcc agacgactac ctggcccgtc
     2161 aacgcaggag aaggggataa tcttactgac taaagcgcat ctgctgttca tggcttatca
     2221 cgcgcggctg gcgttccaga atacgagtgt tgcgtgcggc ttcttcgccc agccaggtgg
     2281 tgttggtgcg tgaaccttcg gcgcgatatt tttctaagtc cgacaaacgt accatataac
     2341 cggtaacgcg aaccagatcg ttaccgctga cattggcggt aaattcacgc attccggctt
     2401 taaaggcacc gaggcaaagc tgtaccagtg cctgcgggtt acgtttgatg gtttcgtcga
     2461 gcgtcagaat gtcgctgatg ccggaataat aataagcatg atgcggggcg acagtttgca
     2521 gatgggtgat cggatctggc tcatcgccat acggcaaacg cgcgcccggc gtggtgccaa
     2581 tatcggaact gatccccgac tgtgcgtgta acatggcgcg tttttgccat ccatatttca
     2641 cgggggtatt ggcgacaaac tccgccagtt gcgcgctgat gcgataaccc acttcatttg
     2701 cggcggcttc tttaccgtaa cgtgcggcaa tcccttcttt ctcacacagc aagttaaccg
     2761 cttccgccag cccatacatg ccaaacattg gcacaaaacg ttcagggtta atcagccctt
     2821 ctttcaccag gaagctattc tcaaagaagt gtgattgttg atagaggaat tcacaccgcg
     2881 catcgatgat ggcgatctgc tgctggcagt agtgcggtag agtgcgcgta aagaagtcat
     2941 ccagcgattc gctgcgctcg gcaatggctt tcaggttaag gcgtaccagc gtgctgccac
     3001 cacccgccag cggcagtgag ttgtaacagc tcacaatccc gtagccccct tttgtgaaaa
     3061 ttttatcatg caccggaccg ttggcgatgt gcggtttgct acattcacag atgttcttcg
     3121 ccacttccag cagcaggtca tcaggggtga tttcaggatc gtagataaag gtcaggttcg
     3181 gtgaaacctg cttcaactct gcgtctgcac gtaagattgc acgggtaatg ggcgaatcag
     3241 acgggccgat attggcgtgc ataaaggcgt ctggcagggt tctgtcgagg taacgccaga
     3301 aacgttttat tcgaacatcg atctcgtctt gtgttagaat tctaacatac ggttgcaaca
     3361 acgcatccag ttgccccagg tagaccggca tcgatgtgac cgacggtacg tggtggtaaa
     3421 gaatggtcag cagagagagt gcgtcatcaa gatctttcgc gccttccagc tccagccatt
     3481 cggaaccgtt cgccagaaaa cgggcgtaat cgggtaagac atagcgcggt ttgtacggcg
     3541 catgaccttc aaacatatcg cagattacac cctcatccag cgcgcggcgg gcttcggcag
     3601 gaagctgcgg ataaggcaga ttgttttctg cttccagagc cagaaaatgg cgcttctgct
     3661 ccgggctaag cactgggctg gtgacaattt gctggcaacg ttgttgcagt gcattttcat
     3721 gagaagtggg catcttcttt tccttttatg ccgaaggtga tgcgccattg taagaagttt
     3781 cgtgatgttc actttgatcc tgatgcgttt gccaccactg acgcattcat ttgaaagtga
     3841 attatttgaa ccagatcgca ttacagtgat gcaaacttgt aagtagattt ccttaattgt
     3901 gatgtatatc gaagtgtgtt gcggagtaga tgttagaata ctaacaaact cgcaaggtga
     3961 attttattgg cgacaagcca ggagaatgaa atgactgatc tgaaagcaag cagcctgcgt
     4021 gcactgaaat tgatggacct gaccaccctg aatgacgacg acaccgacga gaaagtaatt
     4081 gctctgtgtc atcaggccaa aactccggtc ggcaataccg ccgctatctg tatctatcct
     4141 cgctttatcc cgattgctcg caaaacgctg aaagagcagg gcaccccgga aatccgaatc
     4201 gctacggtaa ccaacttccc acacggtaac gatgacatcg aaatcgcgct ggcagaaacc
     4261 cgtgcggcaa tcgcctacgg tgctgatgaa gttgacgttg tgttcccgta ccgcgcgctg
     4321 atggcgggta acgagcaggt tggttttgac ctggtgaaag cctgtaaaga ggcttgcgcg
     4381 gcagcgaatg tactgctgaa agtgatcatc gaaaccggcg aactgaaaga cgaagcgctg
     4441 atccgtaaag cgtctgaaat ctccatcaaa gcgggtgcgg acttcatcaa aacctctacc
     4501 ggtaaagtgg ctgtgaacgc gacgccggaa agcgcgcgca tcatgatgga agtgatccgt
     4561 gatatgggcg tagaaaaaac cgttggtttc aaaccagcgg gcggcgtgcg tactgcggaa
     4621 gatgcgcaga aatatctcgc catcgcagat gaactgttcg gtgctgactg ggcagatgcg
     4681 cgtcactacc gctttggtgc ttccagcctg ctggcaagcc tgttgaaagc gctgggccac
     4741 ggtgacggta agagcgccag cagctactaa gtaagatgct ttacgcctga tgcgctgcgc
     4801 ttatcaggcc tacgagacgt atctacccgt aggccggata aggcgtagac gcatccggca
     4861 aaagccgcct catactcttt tcctcgggag gttaccttgt ttctcgcaca agaaattatt
     4921 cgtaaaaaac gtgatggtca tgcgctaagc gatgaagaaa ttcgtttctt tatcaacggt
     4981 attcgcgaca acactatctc cgaagggcag attgccgccc tcgcgatgac cattttcttc
     5041 cacgatatga caatgcctga gcgtgtctcg ctgaccatgg cgatgcgaga ttcaggaacc
     5101 gttctcgact ggaaaagcct gcatctgaat ggcccgattg ttgataaaca ctccaccggc
     5161 ggcgtcggcg atgtgacttc gctgatgttg gggccgatgg tcgcagcctg cggcggctat
     5221 attccgatga tctccggtcg cggcctcggt catactggcg gtacgctcga caaactggaa
     5281 tccatccctg gcttcgacat tttcccggat gacaaccgtt tccgcgaaat tattaaagac
     5341 gtcggcgtag cgattatcgg ccagaccagc tcactggctc cggcggataa acgtttctac
     5401 gcgacccgtg atattaccgc aaccgtggac tccatcccgc tgatcactgc ctcgatcctg
     5461 gcgaagaaac tggcggaagg tctggatgcg ctggtgatgg acgtgaaagt gggtagcggc
     5521 gcgtttatgc cgacctacga actctctgaa gcccttgccg aagcgattgt tggcgtggct
     5581 aacggcgctg gcgtgcgcac caccgcgctg ctcaccgata tgaatcaggt actggcctcc
     5641 agtgcaggta acgcggttga agttcgtgaa gcggtgcagt tcctgacggg ggaatatcgt
     5701 aacccgcgtc tgtttgatgt cacgatggcg ctgtgcgtgg agatgcttat ctccggcaaa
     5761 ctggcgaaag atgacgccga agcgcgcgcg aaattgcaag cagtgctgga caacggtaaa
     5821 gcggcagaag tctttggtcg tatggtagcg gcacaaaaag gcccaaccga cttcgttgag
     5881 aactacgcga agtatctgcc gacagcgatg ctgacgaaag cagtctatgc tgataccgaa
     5941 ggttttgtca gtgaaatgga tacccgcgcg ctggggatgg cagtggttgc aatgggcggc
     6001 ggtcgtcgtc aggcatctga caccattgat tacagcgtcg gctttactga tatggcgcgt
     6061 ttgggcgacc aggtagacgg tcagcgtccg ctggctgtta tccacgcgaa agacgaaaac
     6121 agctggcagg acgcggcgaa agcggtgaaa gcggcaatta aacttgccga taaagcaccg
     6181 gaaagcacac caactgtcta tcgccgtatc agcgaataac ggtatactga tctgatcatt
     6241 taaatttgaa gcactgagta cggagaacat atgaaacgtg catttattat ggtgctggac
     6301 tcattcggca tcggcgctac agaagatgca gaacgctttg gtgacgtcgg ggctgacacc
     6361 ctgggtcata tcgcagaagc ctgtgccaaa ggcgaagctg ataacggtcg taaaggcccg
     6421 ctcaatctgc caaatctgac ccgtctgggg ctggcgaaag cacatgaagg ttctaccggt
     6481 ttcattccgg caggaatgga cggcaacgct gaagttatcg gcgcgtacgc atgggcgcac
     6541 gaaatgtcat ccggtaaaga taccccgtct ggtcactggg aaatcgccgg tgtcccggtt
     6601 ctgtttgagt ggggatactt ctccgatcac gaaaacagct tcccgcaaga gctgctggat
     6661 aaactggttg aacgcgctaa cctgcctggt tatctcggta actgccactc ttccggtacg
     6721 gtcattctgg atcagctggg cgaagagcac atgaaaaccg gcaagccgat tttctatacc
     6781 tccgctgact ccgtgttcca gattgcctgc cacgaagaaa cctttggctt ggataaactc
     6841 tacgaactgt gcgaaatcgc ccgtgaagag ctgaccaacg gcggctacaa catcggtcgt
     6901 gttatcgctc gtccgtttat tggcgacaaa gccggtaact tccagcgtac cggtaaccgt
     6961 cacgacctgg ctgttgagcc gccagcaccg accgtgctgc agaaactggt tgatgaaaaa
     7021 cacggtcagg tggtttctgt cggtaaaatt gcagacatct acgccaactg cggtatcacc
     7081 aagaaagtga aagcaactgg cctggacgcg ctgtttgacg ccaccatcaa agagatgaaa
     7141 gaagcgggtg ataacaccat cgtcttcacc aacttcgttg acttcgactc ttcctggggc
     7201 caccgtcgcg acgtcgcagg ttatgcggcg ggtctggaac tgttcgaccg ccgtctgccg
     7261 gagctgatgt ctctgctgcg cgatgacgac atcctgatcc tcactgctga ccacggttgt
     7321 gatccgacct ggaccggtac tgatcacacg cgtgaacaca ttccggtact ggtttacggc
     7381 ccgaaagtaa aaccgggctc actgggtcac cgtgaaacct tcgcggatat cggccagact
     7441 ctggcaaaat attttggtac ttctgatatg gaatatggca aagccatgtt ctgataatgg
     7501 gtgcggcttg atgccctcac cccggccctc tccctcaggg agagggtgaa aacatgtaga
     7561 taaggataaa acaatggcta ccccacacat taatgcagaa atgggcgatt tcgctgacgt
     7621 agttttgatg ccaggcgacc cgctgcgtgc aaagtatatt gctgaaactt tccttgaaga
     7681 tgcccgtgaa gtgaacaacg ttcgcggtat gctgggcttc accggtactt ataaaggccg
     7741 caaaatttcc gtaatgggtc acggtatggg tatcccgtcc tgctccatct acaccaaaga
     7801 actgatcacc gatttcggcg tgaagaaaat tatccgcgtg ggttcctgtg gcgcagttct
     7861 gccgcacgta aaactgcgcg acgtcgttat cggtatgggt gcctgcaccg attccaaagt
     7921 taaccgcatc cgttttaaag atcatgactt tgccgctatc gctgactttg acatggtgcg
     7981 taacgcggta gacgcggcta aagcactggg cattgatgct cgcgttggta acctgttctc
     8041 cgctgacctg ttctactctc cggacggcga aatgttcgac gtgatggaaa aatacggcat
     8101 cctcggcgtg gaaatggaag cggctggtat ctacggcgtc gctgcagaat ttggcgcgaa
     8161 agccctgacc atctgtaccg tgtctgacca catccgcact cacgagcaga ccactgccgc
     8221 tgagcgtcag accaccttca acgacatgat caaaatcgca ctggaatccg ttctgctggg
     8281 cgataaagag taattgtgtt tcgctgaaag gcgattgtcg tgtgaagccg gagtgggaga
     8341 ctgctccggc tttttagtat ctattcatca atagcaactg atttatagat ttactattat
     8401 ttttcatatg gatagaaaat aaaatctatt caagtatcta ttcacgaatc tattcattta
     8461 tgttcagccg tcatccagga agggcatatc agcaactctg gtggacaacc ataaatttct
     8521 gccattttgc ttttgatgtt tgagctaaac cgttccgtct tttcgatcta ttttagagaa
     8581 ttaatcgaga tacctaacat tttacttact tcagcaagcg ataatttatg cctgatacgc
     8641 acggcggcta gccaggaaat acgctggctt aacattatat tgataatctc ccgctcctgg
     8701 ttttccagat cgatcatatt cccttccttt ggtattttaa tcagcgagtt accttaccgc
     8761 ccccgctatc cacgtcgaca actcccgtag ctctttttcc tggtccggga agtcaaccaa
     8821 cagcgcttcg cactcttgtt gcagcatatc cgcgcggtac aggccgcctt gcagtcgccc
     8881 ggcgagggct tccagcggcg cggggttgag gctgtcggta aacacctggg cgcgggtgat
     8941 atggcctttt tcaacgtcga aatgcagttc cacgccgccc cagctaaagc gttcatccag
     9001 cagatgcgag aatgcaggag cctgaccgaa gttccattcc cagctactct gacgggcaaa
     9061 ggtttcggcg aagtttggca agtctggcgt tttgtccggg gagatgattt ccgcttctac
     9121 acgctcgcca taatgggcga aaaaggcctt ggttatggcc tcgcaaacct gctcatgggt
     9181 gatccccggc agcagctcgg tgaggttggt cacgcgggaa cgcactgagg taatgccttt
     9241 cgccgccagt ttctttttat ccggattgag atagtttgcc aggcggctaa ggtcggcatt
     9301 gagcagcaag gtgccgtggt ggaagccacg atctttggtt tcgcgatagg ccgatcctga
     9361 gactttgcgg tcaccttcgg cggttttcac caccagatcg ttacgcccgg acgcttcggc
     9421 gctgacgcca agcgcgttca gcgcattgag cacaatcgac gtggagatgg ttttatcgta
     9481 ctccggcttg ccagccataa aggtaaagca ggtattgccg agatcgtgga acaccgcgcc
     9541 gccaccgcta ctgcgccgtg ccaggcggac gttatcttct tccatccgcc gggtattaca
     9601 ctctttccac gggttctgcg cgcgaccaat cactaccgtg tcggcattgc gccagagaaa
     9661 cagaacgcgc tgcgtggcgg gcatctggcg aaaaatacac tcctccaccg ccaggttaaa
     9721 ccacgggtcg taagagtcag agatgagcag gcgtaatgtg gacataacga tttcctttct
     9781 tgtagtaatg ggtcactctt ttttctcgct ttcttcctct tccggcaccg gtttgctggc
     9841 ggttaacagg aagggcgatt gctgccagcg ggtacgttta ccctgtagca gcgtgcgggt
     9901 cagcactacg ccgattgcca gtgaaagcag caacatcagg cgtaaaatgt tagtggtgtt
     9961 atccacctgt tgggcttcgg tggcgagcgt atgggtgtcg agcgtcaggc gcagatagcc
    10021 gagcggtccg tttttcccgg caattggttc gacaatctgc tggttaaaat agccgcccgc
    10081 ttttttaccg tcgagcgcca gccggtcgcg cacttcgacg ctttcgcctg aacgggcgat
    10141 aagatcgcct tgttcgtcat acacacccgc gtcgaggatg cggctttcat ccgttaactg
    10201 atcgaggatc gcctgaatgc gtttttcatc tggtgagtcg gtacgcatca gcggtgcaac
    10261 gttcagcgtc acctgacgcg ccagggtgcg ggccagttct tccagctgtg gattacgctg
    10321 tcgctggtgg ttttgactaa accatgacgc cccctgcatc agtgccacta acaaggcaag
    10381 acagaacagg acaatcactg cccgatgcag ccggaatttc agttttgtgc gagccatctt
    10441 ccaccctttg aaaatttgag acttaatgtt gccagaagca atggatacaa ggtagcctca
    10501 tgcgttattt tccctgcttc gaacgatttt acaggagcct taatgcctaa cattacctgg
    10561 tgcgacctgc ctgaagatgt ctctttatgg ccgggtctgc ctctttcatt aagtggtgat
    10621 gaagtgatgc cactggatta ccacgcaggt cgtagcggct ggctgctgta tggtcgtggg
    10681 ctggataaac agcgtctgac ccaataccag agcaaactgg gtgcggcgat ggtgattgtt
    10741 gccgcctggt gcgtggaaga ttatcaggtg attcgtctgg caggttcact caccgcacgg
    10801 gctacgcgcc tggcccatga agcgcaactg gatgtcgccc cgctggggaa aatcccgcac
    10861 ctgcgcacgc cgggtttgct ggtgatggac atggattcca ccgccatcca gattgaatgt
    10921 attgatgaaa ttgccaaact ggccggaacg ggcgagatgg tggcggaagt aaccgaacgg
    10981 gcgatgcgcg gcgaactcga ttttaccgcc agcctgcgca gccgtgtggc gacgctgaaa
    11041 ggcgctgacg ccaatattct gcaacaggtg cgtgaaaatc tgccgctgat gccaggctta
    11101 acgcaactgg tgctcaagct ggaaacgctg ggctggaaag tggcgattgc ctccggcggc
    11161 tttactttct ttgctgaata tctgcgcgac aagctgcgcc tgaccgccgt ggtagccaat
    11221 gaactggaga tcatggacgg taaatttacc ggcaatgtga tcggcgacat cgtagacgcg
    11281 cagtacaaag cgaaaacttt gactcgcctc gcgcaggagt atgaaatccc gctggcgcag
    11341 accgtggcga ttggcgatgg agctaatgac ctgccgatga tcaaagcagc agggctgggg
    11401 attgcctacc atgccaagcc aaaagtgaat gaaaaggcgg aagtcaccat ccgtcacgct
    11461 gacctgatgg gggtattctg catcctctca ggcagcctga atcagaagta attgctcgcc
    11521 cgccatcctg cgggcggcac agcattaacg aggtacaccg tggcaaaagc tccaaaacgc
    11581 gcctttgttt gtaatgaatg cggggccgat tatccgcgct ggcaggggca gtgcagtgcc
    11641 tgtcatgcct ggaacaccat caccgaggtg cgtcttgctg cgtcgccaac ggtggcgcgt
    11701 aacgagcgtc tcagcggcta tgccggtagc gccggggtgg caaaagtcca gaaactctcc
    11761 gatatcagcc ttgaagagct gccgcgtttt tccaccggat ttaaagagtt cgaccgcgta
    11821 ctaggcggcg gcgtggtgcc aggaagtgcc attctgattg gcggtaaccc tggtgcgggg
    11881 aaatccacgc tgttgctgca aacgctgtgc aaactggccc agcagatgaa aacgctgtat
    11941 gtcaccggcg aagagtcgct gcaacaggtg gcaatgcgcg ctcatcgact tggcctgccg
    12001 actgacaatc tcaatatgtt gtcggaaacc agcatcgagc agatctgcct gattgccgaa
    12061 gaagagcaac cgaaactgat ggtaattgac tcgatccagg tgatgcatat ggcggatgta
    12121 caatcatcgc ctggtagcgt ggcgcaggtg cgtgaaacgg cggcttatct gacgcgcttc
    12181 gccaaaacgc gcggtgtggc gattgtcatg gttgggcacg taaccaaaga tggctcgctg
    12241 gctggcccta aagtgctgga acactgtatc gactgttcgg tgcttctgga tggtgatgcc
    12301 gactcccgtt ttcgcacctt gcgcagccat aaaaaccgct tcggtgcggt gaatgagctg
    12361 ggcgtcttcg cgatgaccga acaggggctg cgtgaagtca gcaacccttc ggcaattttc
    12421 ttaagtcgcg gagatgaagt gacctccggc agctccgtga tggtggtgtg ggaaggaacg
    12481 cgtccgttgc tggtggagat tcaggcgctg gtcgatcact cgatgatggc gaatccgcgc
    12541 cgcgtggcag tggggctgga acaaaaccgt ctggcaatcc tgctggctgt cttgcaccgt
    12601 cacggtggtc tgcaaatggc cgatcaggat gtatttgtga acgtggtcgg cggcgtgaag
    12661 gtaaccgaaa ccagtgccga tttagcgtta ctgctggcga tggtttccag cctgcgtgac
    12721 agaccgctgc cacaggatct ggtggtgttt ggtgaagtcg ggctggcagg ggagatccgc
    12781 ccggtgccca gcggtcagga acgaatttca gaagcggcga aacacggttt tcgccgggcg
    12841 attgttccgg cggctaacgt accgaaaaaa gcgccggaag ggatgcagat ttttggcgtt
    12901 aaaaaactct ccgacgcgct tagcgtgttc gacgacttat aatgagagat acggagggag
    12961 atatgtcgtc atttgattac ctgaaaactg ccatcaagca acagggctgc acgctacagc
    13021 aggtagctga tgccagcggt atgaccaaag ggtatttaag ccagttactg aatgccaaaa
    13081 ttaaaagccc cagcgcgcaa aagctggagg cgttgcaccg ttttttgggg cttgaatttc
    13141 cccgacagaa gaaaaccatt ggtgtcgtat tcggtaagtt ctacccgctg cataccggac
    13201 atatctacct tatccagcgc gcctgtagcc aggttgacga gctgcatatc attatgggtt
    13261 ttgacgatac ccgcgaccgc gcgttgtttg aagacagcgc catgtcgcag cagcccacgg
    13321 tgccggatcg tctgcgctgg ttattacaaa cttttaaata tcagaaaaat attcgcattc
    13381 atgctttcaa cgaagagggc atggagccgt atccgcacgg ctgggatgtg tggagcaacg
    13441 gcatcaaaaa gtttatggct gaaaagggga ttcagccgga cctgatctac acctcggaag
    13501 aagccgatgc gccacagtat atggaacatc tggtgatcga tacggtgctg gtcgatccga
    13561 aacgtacctt tatgagtatc agcggtgcgc agatccgcga aaacccgttc cgctactggg
    13621 aatatattcc tacggaagtg aagccgttct ttgtacgtac cgtggcgatc cttggtggcg
    13681 agtcgagcgg taaatccacc ctggtaaaca aacttgccaa tatcttcaat accaccagtg
    13741 cgtgggaata tggtcgcgat tatgtctttt cacacctcgg cggtgatgag atcgcattgc
    13801 agtattccga ttacgataaa atcgcgctgg gccacgcaca atacattgat tttgcagtga
    13861 aatatgccaa taaagtggcg tttattgaca ctgattttgt cactacccag gcgttctgca
    13921 aaaagtacga agggcgtgag catccgttcg tacaggcgtt gattgatgaa taccgtttcg
    13981 atctggtgat cctgctggag aacaacacgc cgtgggtggc ggatggttta cgcagcctcg
    14041 gcagttcggt ggatcgcaaa gagttccaga acttgctggt ggagatgctg gaagagaaca
    14101 atatcgaatt cgtgcgggtt gaagaggacg attatgacag ccgtttcctg cgctgcgtgg
    14161 agctggtgcg ggagatgatg ggggagcaga gataaccgcg atgaaacggc tcaaaggcga
    14221 ggtataaaat aagttttttg atgagttgaa aacctctctg gaagaggctg tcgagattaa
    14281 acaaggttta aaagaatcag cacgggtgac ccgctacgaa tttgcggata ctaaggctgt
    14341 tgtaggccgg aaaaggcgtt cacgccgtat ccggcattcg gtgctcaatg cctgatgcga
    14401 cgcttaccgc gtcttatcag gcctggagat tcacaacgta ttgaaatttc ttggtttgta
    14461 ggccggataa ggcgttcacg ccgcatccgg cattttacgc attacttcgc aatacgcttg
    14521 tacttgatac gcttcggctc cagtgcgtct gcgcccagcg tgcgtttctt gtactcttcg
    14581 tactcggtaa agttaccttc gaagaactca actttacctt catcctggta atccagaatg
    14641 tgcgtggcga tacggtcgag gaaccaacgg tcgtgcgaga taaccatcgc gcagcccggg
    14701 aactccagca gggcgttttc cagcgcgcgc agggtttcga tatccaggtc attggttggt
    14761 tcgtcgagca gcagcatgtt gccgccaacc tgcagcagct tcgccagatg cagacgaccg
    14821 cgctcaccac cggaaagttc accaacgcgt ttaccctgat caaccccttt aaagttaaag
    14881 cggccaacgt aggcgcggct tggcatctcg gtgttgccga tcttcataat atccagcccg
    14941 ccggaaactt cttcccaaac ggttttgctg ttatccattg agtcacggaa ctgatcaacc
    15001 gatgccagtt ttaccgtttc acctaaagtg atggtgccgc tgtccggctg ttcctgacca
    15061 gagatcatac ggaacagggt cgatttaccc gcgccgttcg gaccgatgat cccgacgatt
    15121 gcccctttcg ggatcgagaa gctcaggtca tcaatcagca ggcgatcacc gtaggattta
    15181 cgcaggttgc tgacttccag cactttatcg cccagacgcg gtccaggtgg aataaacagt
    15241 tcgttggttt cgttacgttt ctgatattcg gtgctgttca gctcttcaaa gcgtgccaga
    15301 cgtgctttac ctttcgactg gcggccttta gttccctgac gtacccactc cagctctttc
    15361 tcgatcgact tacgacgcgc cgcttcttgt gaagcttcct gcgccaggcg ctgatctttc
    15421 tgctccagcc aggaggagta gttgccttcc cacggaatac cttcaccgcg gtcaagttcg
    15481 aggatccagc ctgcaacgtt atcgaggaag taacggtcgt gagtaatcgc cacaacggtg
    15541 ccttcgaagt cgtgcaggaa gcgttccagc caggccacgg attcggcatc caggtggttg
    15601 gtcggttcgt cgagcagcag catgtctggt ttttccagaa gcaggcggca caacgccacg
    15661 cgacgacgct caccaccgga gaggttagcg attttcgcgt cccaatctgg cagacgcagc
    15721 gcatccgccg cacgctccag ctgtacgttc aggttatgac cgtcgtgagc ctgaatgatc
    15781 tcttccagac ggccttgttc agcggccagc ttgtcaaaat cggcatccgg atcggcgtac
    15841 agcgcataca cttcatccag gcgtttcagg gcgttaacca cttcagaaac cgcttcttca
    15901 atggactcac gcacggtgtg ttccgggttc agctgcggtt cctgcggcag gtaaccaatc
    15961 ttgatgtctg gctgcggacg cgcttcacct tcgatgtctt tatcaatgcc cgccataatg
    16021 cgcagcaggg tagacttacc cgcgccgttc agacccagga caccaatttt tgccccaggg
    16081 aagaaactca gagagatgtt tttcaaaata tgacgtttcg gcggaacaac tttgccgaca
    16141 cgatgcatgg tataaacgaa ttgagccacg ttggacttcg cctctatgtt tatcgtgatg
    16201 atgagttttc aaaggcgaag tgtagccttt ttccccgcct aatcccagtt ggtcgatcac
    16261 cttcgcagta aaagtaaaaa agtgtccgta acgtggcgta aacggcaatg actggttagc
    16321 ataaatctat tacgcggcat gacgctgcat tgatgtattt acacttagag gatgcgcttg
    16381 tggaaaaagc caaacaagtt acctggcggc tgttggctgc cggtgtctgt ctgctgacgg
    16441 tcagcagcgt ggcgcgagcc gactcactgg atgagcagcg tagccgttac gcgcaaatca
    16501 agcaggcctg ggataatcga caaatggatg tggtcgaaca aatgatgcct ggactgaagg
    16561 attatccgct ttatccctac ctggaatacc gccagatcac cgatgatctg atgaatcaac
    16621 cggcggtgac ggtcactaac tttgttcgcg ctaaccccac gcttcctccc gctcgcacgc
    16681 tgcaatctcg tttcgtcaat gaactggcgc ggcgtgaaga ctggcgtggc ttgttagcct
    16741 ttagcccgga aaagcccgga actaccgaag cgcaatgtaa ttactactat gcgaaatgga
    16801 acaccgggca gagtgaagaa gcctggcaag gggcgaaaga gctgtggcta accggcaaga
    16861 gccagcctaa cgcctgtgac aagttattta gcgtctggcg tgcgtcaggt aaacaagatc
    16921 cgctggcgta tttagagcgt atccgtctgg cgatgaaagc gggtaacact ggcctggtaa
    16981 cagtgctggc agggcagatg cctgccgatt accagactat cgcctcggca atcatttcac
    17041 tggcgaacaa ccctaatacg gtactgacct tcgcgcgtac aaccggagcg accgatttta
    17101 cccgtcaaat ggcagcggtg gcgtttgcca gtgtggcgcg gcaggatgct gaaaatgcac
    17161 ggctgatgat cccgtcgctt gcccaggcgc agcagcttaa tgaagatcag attcaggagc
    17221 tgcgcgatat cgtcgcctgg cgtttgatgg gcaacgatgt caccgacgag caggcgaaat
    17281 ggcgcgatga cgccattatg cgctcgcaat ccacgtcgtt gattgaacgc cgtgtgcgaa
    17341 tggcacttgg caccggcgat cgtcgcggcc tgaatacctg gctggcgcgt ctgccgatgg
    17401 aagcaaaaga gaaagatgaa tggcgttact ggcaggcgga tttattgctg gaacgcggac
    17461 gtgaagctga agcgaaagag attttgcatc aactcatgca acagcgtggt ttctacccga
    17521 tggttgcagc acaacgcatc ggagaagagt atgagctgaa gattgataaa gcgccgcaga
    17581 atgttgacag cgccctgact caggggccgg agatggcgcg cgtgcgcgag ttgatgtact
    17641 ggaatctcga taacaccgcg cgtagcgagt gggccaatct ggtgaagagc aagtcaaaaa
    17701 cagagcaggc tcaactggcg cggtatgctt tcaacaacca atggtgggat cttagcgttc
    17761 aggcaacgat cgccgggaag ctatgggatc atctggaaga gcgattcccg ctggcttaca
    17821 acgatctttt caaacgctac accagcggta aggagatccc gcaaagctat gcgatggcga
    17881 ttgcccgtca ggagagcgcc tggaatccaa aagtgaaatc accggtaggg gccagcggcc
    17941 tgatgcagat tatgcctggt acagcgaccc atacggtgaa gatgttctct attcccggtt
    18001 atagtagccc tgggcaattg ctggatccgg aaacgaatat caacattggc accagttatc
    18061 tgcaatatgt ttatcagcag tttggcaata accgtatttt ctcctcagca gcttataacg
    18121 ccggaccagg gcgggtgcga acctggcttg gcaacagcgc cgggcgtatc gacgcagtgg
    18181 catttgtcga gagtattccg ttctccgaga cgcgcggtta tgtgaagaac gtgctggctt
    18241 atgacgctta ctaccgctat ttcatggggg ataaaccgac gttgatgagc gccacggaat
    18301 ggggacgtcg ttactgatcc gcacgtttat gatatgctat cgtactcttt agcgagtaca
    18361 accgggggag gcattttgct tcccccgcta acaatggcga catattatgg cccaacaatc
    18421 accctattca gcagcgatgg cagaacagcg tcaccaggag tggttacgtt ttgtcgacct
    18481 gcttaagaat gcctaccaaa acgatctcca tttaccgttg ttaaacctga tgctgacgcc
    18541 agatgagcgc gaagcgttgg ggactcgcgt gcgtattgtc gaagagctgc tgcgaggcga
    18601 aatgagccag cgtgagttaa aaaatgaact cggcgcgggc atcgcgacga ttacgcgtgg
    18661 atctaacagc ctgaaagccg cgcctgtcga gctgcgccag tggctggaag aggtgttgct
    18721 gaaaagcgat tgattttgta gcctggtaag acgcgtcagc gtcgcatcag gcgcttaata
    18781 caccgcatta tgaaacggac tcagcgccag gatcaccgcc tggtgataga cgctggcgcg
    18841 agtgagtttc ccggcggtaa acacgccgat cgccccttcc ttacgaccaa tctcatcaat
    18901 accggtataa cgcgacatta cgggaccaag tgcctcacct tcacgcactt tctgcagaat
    18961 caccgcaggc aacggcaaag tagccgaacg cgcctcgccg cgctggctgg cgttttcaat
    19021 caccacccaa ctgaaagtgc tgtcaccatc gatgccagct tcaatcgcca cccaaaaatc
    19081 agcctctgga agtaaacggc gggcatttgc tacccgattt cgtgcgccag cgcgcgtttc
    19141 ctcactgcca aagggttgtt ccggtacacc gctctcgacg gcaacggatg caatatggca
    19201 ggatccttcg ccgaagatct cgtgaaatgc ctgcagaatg gcctgaattt tagcgggatt
    19261 ggtggtcgca cagacaactt ggtgcataat cagcattact cagaaaatta acgttacagc
    19321 agtatacgga aaaaaagcat gttacaggta tacctagtcc gccacggtga aacgcagtgg
    19381 aacgccgagc gacgtattca gggccagtct gacagcccgc tgaccgccaa aggtgagcaa
    19441 caggcgatgc aggtggcaac ccgtgccaaa gagcttggca ttacgcatat catcagtagc
    19501 gatttaggac gcacccggcg tacggcggaa atcatcgccc aggcctgcgg ctgtgacatc
    19561 atctttgatt ctcgcctgcg tgaattaaac atgggtgtgc tggaaaaaag acatatcgat
    19621 tctctgaccg aagaagaaga gaactggcgt cggcagctgg tcaatggcac cgttgacggg
    19681 cgtattcctg aaggcgagtc aatgcaggag ctcagcgatc gcgtcaatgc ggcactggaa
    19741 tcttgccggg acttaccgca gggaagccga ccgctgctgg tgagccatgg tattgcactg
    19801 ggatgtctgg tgagtacgat tctcggatta ccagcatggg cagagcgccg cttacgtctg
    19861 cgtaactgtt ctatttcgcg cgtggattat caggaaagcc tgtggctggc gtctggctgg
    19921 gttgttgaaa ccgcagggga catctcgcat ctggacgccc ctgcattaga tgagttgcag
    19981 cgttaacgac ggatcggaat cagcagttca cagcgcagat taattgggcg atctcccgct
    20041 ttggcgtctt ctgccgggta gtatcgctca atatcctgac ctttacggcg cgtcaggttg
    20101 agcattggca tgcacgttcc gtataccgta aggataaact cctgcacgcc ggttcccaga
    20161 ccttcatagg taaacatcac atattcgccg ccctgcagca tcaccggatg ccccgtcagt
    20221 acatagccat ctgcctgatc ctgggctaac gcggtggtat agaatacctc ttgttcgtcg
    20281 tctttatcct gactcggacg cgt
//
LOCUS       DAFATA010000082        20235 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFATA010000082 DAFATA010000000
VERSION     DAFATA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.083"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(65..1309)
                     /gene="mtr"
                     /locus_tag="LGJ20_003465"
     CDS             complement(65..1309)
                     /gene="mtr"
                     /locus_tag="LGJ20_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBK2387482.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="LGJ20_003466"
     CDS             complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="LGJ20_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBK2387483.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="LGJ20_003467"
     CDS             complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="LGJ20_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBK2387484.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="LGJ20_003468"
     CDS             complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="LGJ20_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBK2387485.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="LGJ20_003469"
     CDS             complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="LGJ20_003469"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK2387486.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="LGJ20_003470"
     CDS             complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="LGJ20_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK2387487.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7325..8269)
                     /gene="truB"
                     /locus_tag="LGJ20_003471"
     CDS             complement(7325..8269)
                     /gene="truB"
                     /locus_tag="LGJ20_003471"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK2387488.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="LGJ20_003472"
     CDS             complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="LGJ20_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK2387489.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8834..11506)
                     /gene="infB"
                     /locus_tag="LGJ20_003473"
     CDS             complement(8834..11506)
                     /gene="infB"
                     /locus_tag="LGJ20_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK2387490.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVIEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="LGJ20_003474"
     CDS             complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="LGJ20_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK2387491.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="LGJ20_003475"
     CDS             complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="LGJ20_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK2387492.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13705..13781)
                     /locus_tag="LGJ20_003476"
     tRNA            complement(13705..13781)
                     /locus_tag="LGJ20_003476"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="LGJ20_003477"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="LGJ20_003477"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBK2387493.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..17105)
                     /locus_tag="LGJ20_003478"
     CDS             complement(15480..17105)
                     /locus_tag="LGJ20_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBK2387494.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTSEGQG"
     gene            complement(17564..17650)
                     /locus_tag="LGJ20_003479"
     tRNA            complement(17564..17650)
                     /locus_tag="LGJ20_003479"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17614..17616),aa:Leu,seq:gag)
     gene            complement(17665..17997)
                     /gene="secG"
                     /locus_tag="LGJ20_003480"
     CDS             complement(17665..17997)
                     /gene="secG"
                     /locus_tag="LGJ20_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK2387495.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="LGJ20_003481"
     CDS             complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="LGJ20_003481"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK2387496.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19555..>20235)
                     /gene="folP"
                     /locus_tag="LGJ20_003482"
     CDS             complement(19555..>20235)
                     /gene="folP"
                     /locus_tag="LGJ20_003482"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBK2387497.1"
                     /translation="VGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKP
                     EVIRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVF
                     AEVNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLV
                     GMSRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLS
                     AKENKRYE"
BASE COUNT         4940 a         5507 c         4830 g         4958 t
ORIGIN      
        1 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
       61 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
      121 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
      181 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
      241 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
      301 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
      361 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
      421 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
      481 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
      541 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
      601 gtgccatcag cgtaccgtac accaggcatt tcacaatggt tttcggatct ttgccgtaat
      661 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
      721 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
      781 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
      841 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
      901 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
      961 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
     1021 aggcaatgga aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
     1081 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
     1141 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg
     1201 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
     1261 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
     1321 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
     1381 aaaagacaga aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat
     1441 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
     1501 catcacgacg cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
     1561 ggccaccttc acggcgttca ccactgaagt tacgaccgcc ttcacgacgt tcgccaccga
     1621 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgtgcatcg cccagtaact
     1681 gcatgttcat cggcttgttg agaatgcgag tacgcgtaaa gtgttgcagc acttcacccg
     1741 gcataccttt cggcagttcg atggtggagt gagaagcaaa cagcttgatg ttaccaatgt
     1801 aacggctgct gatgtcgcct tcgttagcaa tcgcaccaac gatatgacga acttcaacac
     1861 catcatcgcg gcccacttca atgcggtaca gctgcatatc gccaacatca cgacgttcac
     1921 gacgcggacg atcttcacgg tcaccacgcg ggccacggtc gttacgatcg cgcggaccac
     1981 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggtacgatca
     2041 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatccagct
     2101 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
     2161 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
     2221 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
     2281 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
     2341 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
     2401 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
     2461 tgtccagacg accatctttc aggcgttcca gtgtctgttc acgaagcgcc tggttcatgt
     2521 caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag
     2581 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
     2641 gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag
     2701 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
     2761 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
     2821 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
     2881 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
     2941 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
     3001 gcacgtcata acgctggccg ccgtacagag caaccacatt tacgccgcgc atgtgtttag
     3061 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
     3121 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
     3181 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
     3241 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
     3301 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
     3361 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
     3421 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
     3481 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
     3541 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
     3601 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
     3661 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
     3721 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
     3781 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
     3841 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
     3901 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
     3961 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
     4021 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
     4081 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
     4141 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
     4201 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
     4261 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
     4321 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
     4381 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
     4441 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
     4501 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
     4561 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
     4621 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
     4681 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
     4741 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
     4801 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
     4861 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
     4921 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tatccgggtt
     4981 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
     5041 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
     5101 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcagtg cagagatacc
     5161 gtcgcgggaa cctgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
     5221 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
     5281 ggcagctttg attggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
     5341 catagaagaa gaaccgttgg attcggtgat ttcagacact acacgtacgg tgtacgggaa
     5401 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
     5461 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
     5521 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
     5581 acgagcggta cccagcgttg cagtaaccag tgcctgcgtt tcaccgcggg tgaacagcgc
     5641 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
     5701 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
     5761 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
     5821 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
     5881 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
     5941 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
     6001 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
     6061 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
     6121 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
     6181 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
     6241 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
     6301 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
     6361 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
     6421 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
     6481 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
     6541 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
     6601 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
     6661 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
     6721 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
     6781 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
     6841 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
     6901 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
     6961 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
     7021 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
     7081 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
     7141 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
     7201 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
     7261 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
     7321 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
     7381 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
     7441 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
     7501 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
     7561 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
     7621 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
     7681 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
     7741 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
     7801 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
     7861 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
     7921 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
     7981 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
     8041 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
     8101 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
     8161 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc ggtttatcca
     8221 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
     8281 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
     8341 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
     8401 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
     8461 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
     8521 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
     8581 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
     8641 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
     8701 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
     8761 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
     8821 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
     8881 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
     8941 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
     9001 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcgataacca
     9061 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
     9121 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
     9181 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
     9241 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
     9301 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
     9361 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
     9421 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
     9481 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
     9541 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
     9601 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
     9661 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
     9721 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
     9781 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
     9841 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
     9901 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
     9961 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    10021 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    10081 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    10141 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    10201 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    10261 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    10321 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    10381 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    10441 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    10501 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    10561 tagtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg aagccttgct
    10621 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    10681 cacgatcagc ttttgattca gcgtgtttgt tacctttctt cggacgcgct gctttcgcgt
    10741 tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttcg gcctggcgag
    10801 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    10861 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    10921 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    10981 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    11041 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    11101 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    11161 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    11221 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    11281 caccggtacc aggaatgtta agggtgctgc gtgttttacg ctgcagcgtc aatttgtccg
    11341 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    11401 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    11461 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    11521 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    11581 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    11641 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    11701 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    11761 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    11821 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    11881 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    11941 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    12001 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    12061 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    12121 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    12181 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    12241 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    12301 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    12361 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    12421 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    12481 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    12541 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    12601 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    12661 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    12721 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    12781 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    12841 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    12901 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    12961 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    13021 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    13081 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    13141 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    13201 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    13261 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    13321 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    13381 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    13441 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    13501 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    13561 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    13621 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    13681 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    13741 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaaca tggcaaattc
    13801 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccacgc cacattttgt caacgtttat tgctaatcat
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctccccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcacgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccaattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtag cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgaaaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaggct ccggggatgg cactgctaca tatcgcttat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca tgcacatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcagaggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat taaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    16681 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    16741 aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    16801 ggatcactct ggagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    16861 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    16921 agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag gacgaagaac
    16981 agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaat accaaaaaca
    17041 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    17101 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    17161 tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta gttttttctt
    17221 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    17281 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    17341 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    17401 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    17461 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    17521 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    17581 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    17641 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    17701 agctggttga gtttgttcgg ttttcaccgg tgcactcaga ttttcccatt cgctaccttt
    17761 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    17821 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    17881 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    17941 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    18001 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    18061 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    18121 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    18181 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    18241 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    18301 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    18361 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagtgg atcgccgcta
    18421 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    18481 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    18541 ccgtcaccgg tagtagtttt atccagtagg atcacatgac cggaattctc tgcaccgata
    18601 cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca
    18661 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    18721 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    18781 tgatcgccat cgactttatt gccttcatgg tcgaccataa tcacgcgatc gccatcgccg
    18841 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    18901 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    18961 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    19021 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    19081 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    19141 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    19201 agtttggtgc cgtcgataga gaagaattta atcccattat cgtagaacgg gttatgcgat
    19261 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    19321 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    19381 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    19441 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    19501 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    19561 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    19621 ttctttgacg tcatgaacac gaatgatgtg cgctccttgc attgcggcaa tgaccgcaca
    19681 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    19741 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    19801 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    19861 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    19921 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    19981 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    20041 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    20101 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    20161 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    20221 ggactcgcca ccaac
//
LOCUS       DAFATA010000083        19558 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFATA010000083 DAFATA010000000
VERSION     DAFATA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.084"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..819
                     /gene="rlpA"
                     /locus_tag="LGJ20_003483"
     CDS             <1..819
                     /gene="rlpA"
                     /locus_tag="LGJ20_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBK2387498.1"
                     /translation="GSNLTASGEAFDPTQLTAAHPTLPIPSYARITNLANGRMIVVRI
                     NDRGPYGNDRVISLSRAAADRLNTSNNTKVRIDPIIVAQDGSLSGPGMACTTVAKQTY
                     ALPAPPDLSGGAGASSVSGPQGDILPVSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGV
                     LESSEPTPAPQPVVTAPSTTPATSPAMVTPQAASQSASGNFMVQVGAVSNQARAQQYQ
                     QQLGQKFGVPGRVTQNGAVWRIQLGPFASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            959..2170
                     /gene="dacA"
                     /locus_tag="LGJ20_003484"
     CDS             959..2170
                     /gene="dacA"
                     /locus_tag="LGJ20_003484"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBK2387499.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            2281..2544
                     /locus_tag="LGJ20_003485"
     CDS             2281..2544
                     /locus_tag="LGJ20_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBK2387500.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            2645..3286
                     /gene="lipB"
                     /locus_tag="LGJ20_003486"
     CDS             2645..3286
                     /gene="lipB"
                     /locus_tag="LGJ20_003486"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBK2387501.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            3545..4498
                     /locus_tag="LGJ20_003487"
     CDS             3545..4498
                     /locus_tag="LGJ20_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBK2387502.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            4707..5672
                     /gene="lipA"
                     /locus_tag="LGJ20_003488"
     CDS             4707..5672
                     /gene="lipA"
                     /locus_tag="LGJ20_003488"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBK2387503.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(5773..5976)
                     /gene="tatE"
                     /locus_tag="LGJ20_003489"
     CDS             complement(5773..5976)
                     /gene="tatE"
                     /locus_tag="LGJ20_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBK2387504.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(6105..6893)
                     /locus_tag="LGJ20_003490"
     CDS             complement(6105..6893)
                     /locus_tag="LGJ20_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBK2387505.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            6986..7369
                     /gene="crcB"
                     /locus_tag="LGJ20_003491"
     CDS             6986..7369
                     /gene="crcB"
                     /locus_tag="LGJ20_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBK2387506.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(7424..7633)
                     /gene="cspE"
                     /locus_tag="LGJ20_003492"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(7424..7633)
                     /gene="cspE"
                     /locus_tag="LGJ20_003492"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBK2387507.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(7808..8368)
                     /gene="pagP"
                     /locus_tag="LGJ20_003493"
     CDS             complement(7808..8368)
                     /gene="pagP"
                     /locus_tag="LGJ20_003493"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBK2387508.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRKNIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            8956..10341
                     /gene="dcuC"
                     /locus_tag="LGJ20_003494"
     CDS             8956..10341
                     /gene="dcuC"
                     /locus_tag="LGJ20_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBK2387509.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(10382..11062)
                     /gene="dpiA"
                     /locus_tag="LGJ20_003495"
                     /gene_synonym="citB"
     CDS             complement(10382..11062)
                     /gene="dpiA"
                     /locus_tag="LGJ20_003495"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBK2387510.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(11031..12689)
                     /gene="dpiB"
                     /locus_tag="LGJ20_003496"
     CDS             complement(11031..12689)
                     /gene="dpiB"
                     /locus_tag="LGJ20_003496"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBK2387511.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVMRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            13069..14127
                     /gene="citC"
                     /locus_tag="LGJ20_003497"
     CDS             13069..14127
                     /gene="citC"
                     /locus_tag="LGJ20_003497"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBK2387512.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            14142..14438
                     /gene="citD"
                     /locus_tag="LGJ20_003498"
     CDS             14142..14438
                     /gene="citD"
                     /locus_tag="LGJ20_003498"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBK2387513.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            14435..15343
                     /gene="citE"
                     /locus_tag="LGJ20_003499"
     CDS             14435..15343
                     /gene="citE"
                     /locus_tag="LGJ20_003499"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBK2387514.1"
                     /translation="MISASLQQRKTRTHRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            15354..16886
                     /gene="citF"
                     /locus_tag="LGJ20_003500"
     CDS             15354..16886
                     /gene="citF"
                     /locus_tag="LGJ20_003500"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBK2387515.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            16890..17441
                     /gene="citX"
                     /locus_tag="LGJ20_003501"
     CDS             16890..17441
                     /gene="citX"
                     /locus_tag="LGJ20_003501"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBK2387516.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            17416..18294
                     /gene="citG"
                     /locus_tag="LGJ20_003502"
     CDS             17416..18294
                     /gene="citG"
                     /locus_tag="LGJ20_003502"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBK2387517.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVLNHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            18345..>19558
                     /gene="citT"
                     /locus_tag="LGJ20_003503"
     CDS             18345..>19558
                     /gene="citT"
                     /locus_tag="LGJ20_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBK2387518.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTAT"
BASE COUNT         4822 a         5079 c         4697 g         4960 t
ORIGIN      
        1 ggcagtaacc tgacggcctc tggcgaagct ttcgatccga cacagttgac ggcggcccat
       61 ccaacgcttc cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc
      121 gtggtgcgca ttaatgatcg cggcccctac ggcaacgacc gcgtaatttc gctttctcgc
      181 gcagcagctg accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt
      241 gtcgcccagg atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag
      301 acttacgccc tgcctgcacc tcccgattta agcggtggcg cgggggcaag ttcagtgtct
      361 ggcccgcagg gtgacattct tccggtcagt aactcgacgc taaaaagcga agatccgacc
      421 ggcgcgccgg taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctggtgta
      481 ctggaaagca gcgaaccgac acctgcacca cagcccgttg ttacagctcc gtcgacaacg
      541 cctgcaacct cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac
      601 tttatggtgc aagtcggggc cgttagcaat caggctcgtg cgcaacagta ccaacagcaa
      661 ctgggacaga agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcgtatc
      721 cagcttggcc cattcgccag taaagccgaa gccagtacct tgcagcaacg tttgcaaacc
      781 gaagcccaat tacagtcatt tattaccacc gcgcagtagc gtaaagcagg catctgatgt
      841 gtcaatttct gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt
      901 gcatttgcta tagtagggca ctttttttaa ttccatcacg gatgtcgtag ttcagaccat
      961 gaataccatt ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac
     1021 agcctttatc tctgctgcac atgccgatga cctgaatatc aaaactatga tcccgggtgt
     1081 accgcagatc gatgcggagt cctacatcct gattgactat aactccggca aagtgctcgc
     1141 cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg accaaaatga tgaccagtta
     1201 cgttatcggc caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg
     1261 caacgacgca tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa
     1321 accgggcatg caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa
     1381 cgatgcttgc gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt
     1441 gatgaacagc tacgttaacg ccctgggcct gaaaaacacc cacttccaga cggtacatgg
     1501 tctggatgct gatggtcagt acagctccgc gcgagatatg gcgctgatcg gccaggcatt
     1561 gatccgtgac gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg
     1621 tattcgccag ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggcat
     1681 caaaaccgga cacactgaca aagcaggtta caaccttgtt gcttctgcga ctgaaggcca
     1741 gatgcgcttg atttctgcgg tgatgggcgg acgtactttt aaaggccgtg aagccgaaag
     1801 taaaaaactg ctaacctggg gcttccgttt cttcgaaacc gttaacccac tgaaagtagg
     1861 taaagagttc gcctctgaac cggtttggtt tggtgattct gatcgcgctt cgttaggggt
     1921 tgataaagac gtgtacctga ccattccacg tggtcgcatg aaagatctga aagccagcta
     1981 tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat
     2041 caacttccag cttgatggca aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat
     2101 cccggaaggt aacttcttcg gcaaaatcat tgattacatt aaattaatgt tccatcactg
     2161 gtttggttaa aaattgaaca cttgaaagtg taatttccgt ccccatatac taagcatcag
     2221 taaaaaaact cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac
     2281 atgaaaacca aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg
     2341 gggcaggcgt tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca
     2401 ggtgactaca ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc
     2461 actatcaacg ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc
     2521 gatattgtcc gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac
     2581 tcccctctcc tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc
     2641 cgttttgtat caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat
     2701 ctcccaggct atgcatgaat tcaccgatac ccgcgatgat agtacccttg atgaaatctg
     2761 gctggtcgag cactatccgg tattcaccca aggtcaggca ggaaaagcgg agcacatttt
     2821 aatgccgggt gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg
     2881 gccgggacaa caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg
     2941 tgaactggtg accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga
     3001 agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact
     3061 gggtttacga attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga
     3121 tctttcacca tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat
     3181 atcacaatgg aaacccgaag cgacgactaa taatattgct ccacgtttac tggaaaatat
     3241 tttagcgcta ctaaacaatc cggacttcga atatattacc gcttaattcc atacatcaat
     3301 ggcccaattt acactgggtc attactcata atacctgcta atgctttaca tctccttcgc
     3361 gtttgtatat tttctttatc acttgcagaa atgcggtgcc acgccttgct ccgcaactca
     3421 ccccattgat taacctattg aaatagttga ttatggtgag caaaagcgtt ccgttccaat
     3481 tagacataag attattgtaa ttttttaaat attatttttc catgaataaa attggagtaa
     3541 gtgcgtggat agtaataatc aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa
     3601 accacaaata tttacaacct tacgaaatat tgatcttaac cttctgacta tttttgaagc
     3661 tgtatatgta cataaaggga tcgttaatgc agcgaaagtg cttaatctga ccccctcggc
     3721 aatcagtcag tctattcaga aactgcgcgt tatattccct gacccattgt ttattcgcaa
     3781 aggccagggt gtcactccta ccgcatttgc gatgcatcta catgagtata tcagtcaggg
     3841 ccttgagtcc attcttggcg cgctggatat cgaaggaagc tatgataagc aacgaacgat
     3901 aactattgcg accactccct cggtcggagc cctggtcctt cctgtcatct atcgggcgat
     3961 taaaactcac tatccgcagc ttttactgcg caacccgccc atcagcgacg cggaaaacca
     4021 actcagtcag tttcaaaccg atctcatcat cgataacatg ttttgcacca atcgtacggt
     4081 gcaacatcat gttctgttca ccgacaatat ggtgttaatt tgccgcgagg gaaatccact
     4141 actctcttta gaagatgaca gagagactat cgacaacgct gcgcatgtac ttctgttacc
     4201 ggaagagcaa aatttcagcg gtctgcggca gagagttcaa gaaatgtttc cggaccggca
     4261 aatcaatttc accagctaca acattttgac aatcgctgca ctggttgcca acagtgacat
     4321 gttagcgatt attcccagcc gtttttataa cctgtttagc cgctgctggc cgctggaaaa
     4381 actgcctttt ccgtccttaa atgaggagca aatagatttc tctatccact acaacaaatt
     4441 cagcctgcgt gatcccatcc tgcacggagt aatagatgtt atccgtaatg cattttgaaa
     4501 aagtattgtt acagccagat cacagcgcag aacgataaaa tggcacaatc caacaacaat
     4561 tttacatttt cgcgaccgct ttggctgctt tcaggtccgt ttcaatgata tactgccagt
     4621 cgttaattca aaaatagttg ataattacaa caatctattg aattgaaacg ctttccttcg
     4681 taattcgcaa ctggaacacg cacgctatga gtaaacccat tgtgatggaa cgcggtgtta
     4741 aataccgcga tgccgataag atggccctta tcccggttaa aaacgtggca acagagcgcg
     4801 aagccctgct gcgcaagccg gaatggatga aaatcaagct tccagcggac tctacacgta
     4861 tccagggcat caaagccgca atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct
     4921 cctgccctaa cctggcggaa tgcttcaacc acggcacagc aacgtttatg atcctcggcg
     4981 ctatttgtac ccgccgttgt ccgttctgtg atgttgccca cggtcgcccg gtagctcctg
     5041 atgccaatga accagtgaaa ctggcgcaga ccattgccga tatggcgctg cgttatgtgg
     5101 ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg tgcccagcac tttgcggatt
     5161 gcattactgc cattcgggaa aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt
     5221 tccgtggtcg tatggatcgt gctctggata ttctgactgc aacgccacca gatgtgttca
     5281 accataacct ggaaaacgta ccgcgtattt accgtcaggt acggcctggt gcagattaca
     5341 actggtcgct gaagctgctg gaacgcttta aagaagcgca tccggaaatc ccgaccaagt
     5401 ctggtctgat ggtgggactg ggtgaaacca atgaagaaat tattgaggta atgcgcgacc
     5461 tgcgccgtca tggtgtgacg atgttaacgc tggggcaata tttgcagcca agccgccatc
     5521 acctgccggt tcaacgttac gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc
     5581 tggcgatggg ctttacccat gctgcatgcg gtccgtttgt ccgctcttct taccacgccg
     5641 atttgcaggc gaaagggatg gaagttaagt aatcctgtaa taattactta catctgtctg
     5701 ataaaaaacc cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc
     5761 ctgctcgcca cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc
     5821 ttttttcgcc gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc
     5881 aaggtctccg cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc
     5941 aactactagc agtttggtaa tactaatctc acccatagat accttcttga catataaccg
     6001 gcaacagaac gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa
     6061 tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa aacatcataa taattgcggc
     6121 ggcgcaaagc gacggttgtt taagacgggc agttgcgcgc gcacctgacg cacacgttcg
     6181 ggcgtcactt ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca
     6241 aagggatcta taatccggct ttgaccgata tttttgttcc cgcactcccc cgccgccacc
     6301 atataacagg tggtatccag cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg
     6361 agcggcccgc gaacccaggc ggcaggaagt accaggattt cagctccctg taatgcctgt
     6421 gccagcgcca gctctggaaa gcgtaagtca taacaggtca tcagaccgac cttcatcccc
     6481 tccacctcga gtaacggagc gatttcatta ccagcatcaa cacggcgtga ttcctgaatg
     6541 gcaaatgcat catagagatg caatttggca taacgggcga cgatgtttcc tgcctgtagc
     6601 gccaccagca tattccatgc gcgccccggc gttgaaggaa catgaatcgt cagaattgtc
     6661 gtcatcatgt tacgtttact ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc
     6721 tgtgctgatt taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc
     6781 aggacgaaca gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca
     6841 gcgttctttt cccacacaga tgtaacagca aactgtcctg cggcaaccag cattgccacc
     6901 tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat
     6961 acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg
     7021 tggtacggga agcgtggcga gatggctgtt aagtatgcga tttaacccgc tgcatcaggc
     7081 gattccgttg gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt
     7141 cgcatggttc agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg
     7201 attttgtggc ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca
     7261 agagggtcgc tttggctggg cattactgaa cgttttcgtc aaccttctgg ggtcttttgc
     7321 catgacagca ctggctttct ggctgttttc ggcctcaacc gcacactaaa gaccaaaaaa
     7381 aaacccgctg attaagcggg ttttgaattc ttgctgacgt atcttacaga gcgattacgt
     7441 ttgcagcaga agggcctttg gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag
     7501 caagagtttt aaaaccatta gtctggattg cagagaagtg tacgaacacg tctttgctgc
     7561 cgtcttccgg agtaatgaaa ccgaatcctt tggactcatt aaaccactta acgttacctt
     7621 taatcttaga catcaaaatt acctttacat gaaaaataga cacaaatgct gtgtcggtta
     7681 ccagtacacc aattgtggta cgctttgtcc agtgcaactt tactaaaaag tgataaaagt
     7741 cgcgttattt ttgatgttac ttattttagc tattgatttt aaagaagtta ctaaaacttc
     7801 atttgtctca aaactgaaag cgcatccagg caaagtacac attgccattg ttgtaggtac
     7861 ccggaatgta ggtcatctga aaagtcactg ggccataacc cacggaggcc aatggcagta
     7921 gaaccgggag agggatgtaa ttccagttat cgcgtgccgt tacgccagcg gtgaatccca
     7981 gacctaaatg aaaattttca tccgccagcg gtcgccaggt actttcccat ccgtatccgg
     8041 caatcggttc ccatttgttc cacgagtcct taaatgccat ggcatacagg ccatgccagt
     8101 ttcctttttc atcccaacgc gacaggccaa aaccgccacc ccacggtcgc tcgttatagc
     8161 gatcggtttt ttctttgtcg taagcgaaac gtgcatgcca ggtgatggca ggaatatata
     8221 aatcataatg ttcaggctgt tgccaggttt gtgcaatatt ttttctaaac gttgtcatcc
     8281 actcatctgc gttagcaaaa actttaccaa cgctgattaa ctgaataaaa acaaaggaaa
     8341 agatagcgac atatttactc acgttcattt gtgaccataa aacatttatc aaaaatctac
     8401 tactagcata gcaaagctac aattaacata accttaatag acccaacata aagaataatc
     8461 tgaatagctg ctcatttatt tccgcttgtt caagttaaat atttccgcgc atcgattaaa
     8521 gatgatgcgt cgttttctgc gatgggaata gtcaaaaaag aaaaaccaag tcttttttga
     8581 tgacaaaatg caatcaagga aaaattattt tattttttaa cgagatacaa caatcatctt
     8641 aacgaagtat ataatatata tcgtcaacct gtgctcatat tagattattt tttgagcgca
     8701 cagccgcagc taacaccatt gcaattaaca aatttgcatc aatccaccat caatttgcac
     8761 acattattat gtgataattg ccaaccgcta aatatgcgtt ttgttatcta tgtataaaaa
     8821 cagcaacttc aatgtcttaa tggcagtttt tcttgatttt aatcagcatt catcgacaat
     8881 ttattgggca tattttttcc ttaagcttta ggaatttttt attatttact ttggggcctg
     8941 gagacaggaa aaattatgct gacattcatt gaactcctta ttggggttgt ggttattgtg
     9001 ggtgtagctc gctacatcat taaagggtat tccgccactg gtgtgttatt tgtcggtggc
     9061 ctgttattgc tgattatcag tgccattatg gggcacaaag tgttaccgtc cagccaggct
     9121 tcaacaggct acagcgccac ggatatcgtt gaatacgtta aaatattact aatgagccgc
     9181 ggcggcgacc tcggcatgat gattatgatg ctgtgtggat ttgccgctta catgacccat
     9241 atcggcgcga atgatatggt ggtcaagctg gcgtcaaaac cattgcagta tattaactcc
     9301 ccttacctgc tgatgattgc cgcctatttt gtcgcctgtc tgatgtctct ggccgtctct
     9361 tccgcaaccg gtctgggtgt tttgctgatg gcaaccctat ttccggtgat ggtaaacgtt
     9421 ggtatcagtc gtggcgcagc tgctgccatt tgtgcctccc cggcggcgat tattctcgca
     9481 ccgacttcag gggatgtggt gctggcggcg caagcttccg aaatgtcgct gattgacttc
     9541 gccttcaaaa cgacgctgcc tatctcaatt gctgcaatta tcggcatggc gatcgcccac
     9601 ttcttctggc aacgttatct ggataaaaaa gagcacatct ctcatgaaat gttagatgtc
     9661 agtgaaatca ccaccactgc tcctgcgttt tatgccattt tgccgttcac gccgatcatc
     9721 ggtgtactga tttttgacgg taaatggggt ccgcaattac acatcatcac tattctggtg
     9781 atttgtatgc tgattgcctc cattctggag ttcctccgca gctttaatac ccagaaagtt
     9841 ttctctggtc tggaagtggc ttatcgcggg atggcagatg cgtttgctaa cgtggtgatg
     9901 ctgctggttg ccgctggggt attcgctcag gggcttagca ccatcggctt tattcaaagt
     9961 ctgatttcta tcgctacctc gtttggttca gcgagtatca tcctgatgct ggtattggtg
    10021 attctgacca tgctggcggc agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt
    10081 gttgagatga tcccgaaact ggcgcactct tccggcatta acccggcgta tttgactatc
    10141 ccgatgctgc aggcgtcaaa cctgggtcgt accctatcac ccgtttctgg cgtagtcgtt
    10201 gcggttgccg ggatggcgaa aatctcacca tttgaagtcg taaaacgcac ctcggtgccg
    10261 gtgcttgttg ggctagtgat tgttatcgtt gctacagagc tgatggtgcc aggaacggca
    10321 gcagcggtca caggcaagta aatagtaatg ccggaggcaa gtttcctccg gctttttcat
    10381 gtcacccact gtggtatata cgttgcggtc tgccaacttt gccgtgaaca atttcagcaa
    10441 taatcagatg gcggctggcg caatattcaa gataacgcct ggcagtggtg cggctgatgg
    10501 tcagtgcctg cgccaccgtt tctgccgtat gttgcacacc aggctctttg aacagttttc
    10561 gcaccgcgtt tagcgtcaaa gggtcaatgc cggtcggtag ctcgtcctta ggttcaccgc
    10621 gagcataagc attaaacatc tcatcaattt gcttctggct ggcgctatca atactttcca
    10681 gcatatgttt acgctggcgg aaacgggtta gcgtttgccc cagccgttca taggcaatgg
    10741 gcttaatgag ataatcaaat acaccacaac gtacggcttc agacaccgtt tccatatcgc
    10801 tggctgcagt ggtaaacacc acgtcgccgg gataatgcgc ctgcaccagt tcatgcagta
    10861 aattaatccc tctaccgtca ggaagatagt tatcgagcaa gattagcccc ggcttaaaac
    10921 gctcgatcat cattcgggcc tgcgccaggt ttcccgccag taatatctga ctgaatccgg
    10981 gaatgtgacg aatatattcc gcatgcatct ctgccagcgg cgtttcgtcc tcaacgatca
    11041 atagggttaa tggagctgtc attaggtttc actttcggaa tatagattga aaataaggta
    11101 ccgcagggat cattatcttc gagagtgata acaccaccgc agcgcattac gtagctggca
    11161 atcaagtaca acccaatgcc atgttcaccg ggctcgtcag cacgcgtact gaccccctgc
    11221 tcaaatattt tgtctcgtag agactctgga acgccgcagc cctgatcggc gacttcaatc
    11281 accacatcat cgccttcatc gctgaggaat aattcaacga tcttgtttcc ttcatcgcta
    11341 cgcaggctgg cttcgaaggc gttatcaagt aaattgccaa caatggcggc aaactcggtg
    11401 ctatccagtc ctggcggcag ttgcgaaagc tggctaccgg ggacgatgat catttttagc
    11461 cccagttcgc gggcgcgctg cactttacca aaaagcagcc ccgccacctg gcgatcggca
    11521 aacgcctcgc gcaggctgtc aataagctgt tgctgggcct gagactcccc ctgcaccatc
    11581 gccagcacgc gatcatactc tttcatctgc aacagaccat tgagcgtcga catccaattg
    11641 agatgctcgt gtcgcaatgt acgaaggctc tcaacgtatt gttttatttg cgtcagttgc
    11701 gcattgaggg tggaaatttc gtctttacta cgaaagctga taatggcccc cagcaaatca
    11761 tcacctgaac gaatagcttc ccggttggca ataacgctca gaccgttaaa gttcgccacc
    11821 acatcctgac gtttttcatc aatctgttcg gtaaagaaat cggcgggcct gaccacttca
    11881 gcaatgggtt tacccaacca ttgccgtcca ggggaactca gccccagcat ctttcttgcg
    11941 ttacgattga tggcggtaat gtaaccgtgc ggatccaccg caatcagccc ttcataaacc
    12001 gaactaaaca gcgcctcttg ctgacggacc acccgtgcga tttgctttgg ctccatgccc
    12061 atcatctgcc gacggatatg cgcggccagg aaccacgaca gcaacatcag aatccctaac
    12121 agcacgacaa acacgcctgc catcggtaat aaaaactcag cccgccagct atcgatttta
    12181 ctcaccaggt agccaatcga caccacgccg atgacttttc catcgtcatc aaagattggc
    12241 gttttggcgc gcatcgccat acccattgac cctttcccgg taatgaagta gctctcccct
    12301 ttctccagcg cgccctgttt ggtgaactgc ataggataac caattttctc cggattagga
    12361 tggtaaaggc ggatcgagtg ccggtcgcca atcaccacat aatcaaaatc ggtatctctt
    12421 tgtaatttgt tagcgatggt cgccagccgt ttgtagtcac gcgttttcac cgcagagatg
    12481 acactgtcat tggaggcaat aattttcgcc tgattcattg ccatgtcgcg tacatgaagc
    12541 gttaaatagt cctcaaaact ggccgtaaaa tattgcgcca gggctgcaat gacaaatatt
    12601 gagaacacca gaatcagcaa aaagatacgc agcggaaatg ccagtctttg gaaaaatgca
    12661 aactgtttat tctcgttaag ctgcaacatt gttttccttg cgggttgtcc cgagcctctt
    12721 tgacggatgt aattaaaatg ttgaatcaaa atacatagat attaattatg atcgtggcaa
    12781 attttattca ttaaatcaat taaatcactt aaacctgcga gtcatttcgt aattacatta
    12841 attaaaaaac ttaaaaccat taaataaata aaaaaccact aactctatgt gaaataaatc
    12901 aaaatttcac gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc
    12961 gatcaccccc ctttctccca agtgaaaata aaaggttatc agtttgcaac attgaacaac
    13021 attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat gttcggcaat
    13081 gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc
    13141 ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc
    13201 gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc
    13261 agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc
    13321 gcctatgagc ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg
    13381 ttccgccagt gcggtttttc cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa
    13441 aacagcgcca cgcgactgaa acgctatgcc gaatcgctga aaaaatttcg tcatccaggg
    13501 aacaagattg gctgcattgt gatgaacgcc aatcccttca cgaatggtca ccgttatctg
    13561 attcaacaag ctgcggcaca gtgcgactgg ttgcatctgt ttttagtcaa agaagattct
    13621 tcacgcttcc cctatgaaga ccggttggat ctggtgttaa aaggcaccgc cgatattcca
    13681 cgcctgactg tgcatcgcgg ctccgaatac atcatctccc gcgctacgtt cccttgctac
    13741 ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc
    13801 cgtcagtacc tcgctcccgc gctgggtgta actcaccgct ttgtcggtac tgaacccttt
    13861 tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc
    13921 tccgcaccgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata
    13981 tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg
    14041 gtccctgcag tcacgctgca ttatttgcag aacctgcttg agcactcccg ccaggacgcg
    14101 gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg
    14161 ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc
    14221 aggatatcga cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat gcaattcgca
    14281 ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg
    14341 acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca
    14401 gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg
    14461 taaaactcgc acccaccgca gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag
    14521 caactccttc atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtagcatt
    14581 gcgtgaaaaa gacaccgccc gccgcatggt ttaccacgcg ctgcaacatc cgctgtatcg
    14641 cgatattgaa accattgtgc gtgtcaacgc gctggattcc gaatggggtg tcaacgacct
    14701 ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca
    14761 ggatgttctg gatatcgaaa aagagatcct gcgtatcgaa aaagcctgtg gtcgtgaacc
    14821 cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcagtgga
    14881 aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg
    14941 caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgcac gctgttccat
    15001 tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa
    15061 caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa
    15121 atcgctgatc aacccgcgtc agattgatct gttgcacaac ctctacgcac caacccagaa
    15181 agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct
    15241 cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct
    15301 ggtgctctct cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc
    15361 agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcgctgaat
    15421 gcgatcttgc cgctttccag aactcgccaa agcaaaccta ccaggctgaa aaagcgcgcg
    15481 atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctctggttta caggacggca
    15541 tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg
    15601 acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt
    15661 gccatgcgcc gctggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg
    15721 gcctacgtgg tccactggcg gaagaaatct cccgcggtct gctggcggaa ccggtgcaaa
    15781 tccactctca cggcggtcgt gtgcatctgg tacagagcgg cgaactgaat atcgatgtgg
    15841 ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac accggtaaag
    15901 cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggttgtga
    15961 tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg
    16021 ttgatttaat cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtgcta
    16081 cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg
    16141 tcaactcagg ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc ggcgcatcgc
    16201 tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg
    16261 ccctcggtgg tattaccgcg acgatggttg atctgcacga aaaaggtctg atccgcaaac
    16321 tgctggatgt acagagcttt gacagccacg ctgcgcaatc gctggcccgt aaccccaatc
    16381 acatcgaaat cagcgccaac cagtacgcta actggggttc gaaaggcgca tcggttgatc
    16441 gtctcgacgt ggtggtactg agcgcgctgg aaattgacac ccagttcaac gttaacgtgc
    16501 tgaccggctc tgacggcgta ctgcgtggtg cttccggtgg tcactgcgat accgcgattg
    16561 cctctgcgct ttccatcatc gtcgcgccgc tggtacgcgg tcgtattccg actctggtgg
    16621 ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg
    16681 gtatcgcagt taacccggca cgtccggagc tggcagaacg tctgcaggaa gcgggcatta
    16741 aagtggtttc cattgagtgg ctgcgtgaac gtgcgcgtct gctgaccggt gaaccacagc
    16801 cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg
    16861 atgttgtaca tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc
    16921 atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg
    16981 tctggctcaa gcgtcatcct gttccactgg tctcctttac cgtggttgcg cctgggccga
    17041 ttaaagacag tgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct
    17101 tagccgcaaa acagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc
    17161 cggagggtat gttgagcatt gccgccccgg ctcgcgacct caagctcgcc accattgagc
    17221 ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg
    17281 aaattctctc ccgccgcgac tattcactgc cgcctcgcag ctgcctgttg tgcgagcaaa
    17341 gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg
    17401 aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg
    17461 tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg
    17521 tcaccgaaac caggcctcgt ggatcggatt aactgcggtg cgcataaaga tatggcgctg
    17581 gaagatttcc accgcagcgc gctggcgatt cagggctggc taccccgttt cattgaattt
    17641 ggtgcctgta gtgcggaaat ggcaccagaa gcggtactcc acggattacg cccaattggt
    17701 atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggcagc
    17761 attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct caaccagccg
    17821 gtaacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggtct gaccgatcgc
    17881 gaactgcgta ccaataattc acaactgacg gcaggtcaac ggttgtacca gcagcttggc
    17941 cttaccggcg cacgcggtga agccgaagcg ggttatccac tagtgctcaa ccacgccctg
    18001 ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt gctcgatacc
    18061 ttgctcctac tgatggcgat caacggcgat accaacgttg catcgcgcgg tggcgagggg
    18121 ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc
    18181 cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt
    18241 ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt
    18301 aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag
    18361 ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt cttatccctg
    18421 tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg gcaatgattg
    18481 tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg gttactattt
    18541 gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta
    18601 atgcgcaaaa acaggcgctg aaatggggtc tggctggttt ttccagcacc acggtatggc
    18661 tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca
    18721 ttgccctttt cctggtgaaa ttcatgggca aacgcacgct gacgttgggt tatgcgattg
    18781 tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta
    18841 cggtttttcc ggtcattaaa aacctgccgc cgctgtttaa atcattcccc aacgatccgt
    18901 ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt
    18961 cgtccatgtt tgtcaccggt gcggcaccaa acgtgctggg tctggagttc gtcagcaaaa
    19021 ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta
    19081 tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca
    19141 gtgaagaagt ggcaacctgg gcgggtgatg aactgaaaac catgggtgcg ctgacacgca
    19201 gagagtggac actgattggc cttgtattgc tcagcttagg tttgtgggtt tttggcagtg
    19261 aagtcattaa tgctactgca gttggtctgc tggcagtttc gctaatgctg gccctgcacg
    19321 ttgtgccttg gaaagatatt acccgctata acagcgcatg gaacacgctg gtcaacctgg
    19381 caactctggt tgtgatggct aacggcctga ctcgttctgg ttttattgac tggttcgccg
    19441 gtaccatgag tacgcacctg gaaggattct caccaaacgc aacggtgatt gtactggttc
    19501 tggtgttcta ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccat
//
LOCUS       DAFATA010000084        19037 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFATA010000084 DAFATA010000000
VERSION     DAFATA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.085"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(138..1520)
                     /locus_tag="LGJ20_003504"
     CDS             complement(138..1520)
                     /locus_tag="LGJ20_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBK2387519.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1813..1907
                     /locus_tag="LGJ20_003505"
     tRNA            1813..1907
                     /locus_tag="LGJ20_003505"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1847..1849,aa:Sec,seq:tca)
     gene            2544..3728
                     /locus_tag="LGJ20_003506"
     CDS             2544..3728
                     /locus_tag="LGJ20_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HBK2387520.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            3839..4762
                     /locus_tag="LGJ20_003507"
     CDS             3839..4762
                     /locus_tag="LGJ20_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2387521.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(4766..5584)
                     /gene="nlpA"
                     /locus_tag="LGJ20_003508"
     CDS             complement(4766..5584)
                     /gene="nlpA"
                     /locus_tag="LGJ20_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBK2387522.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            5806..6099
                     /locus_tag="LGJ20_003509"
     CDS             5806..6099
                     /locus_tag="LGJ20_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387523.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(6140..7330)
                     /gene="nepI"
                     /locus_tag="LGJ20_003510"
     CDS             complement(6140..7330)
                     /gene="nepI"
                     /locus_tag="LGJ20_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBK2387524.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(7541..7993)
                     /locus_tag="LGJ20_003511"
     CDS             complement(7541..7993)
                     /locus_tag="LGJ20_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBK2387525.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(8046..9380)
                     /gene="adeQ"
                     /locus_tag="LGJ20_003512"
                     /gene_synonym="yicO"
     CDS             complement(8046..9380)
                     /gene="adeQ"
                     /locus_tag="LGJ20_003512"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBK2387526.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            9555..11321
                     /gene="adeD"
                     /locus_tag="LGJ20_003513"
     CDS             9555..11321
                     /gene="adeD"
                     /locus_tag="LGJ20_003513"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK2387527.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(11366..12757)
                     /gene="uhpT"
                     /locus_tag="LGJ20_003514"
     CDS             complement(11366..12757)
                     /gene="uhpT"
                     /locus_tag="LGJ20_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBK2387528.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(12895..14214)
                     /gene="uhpC"
                     /locus_tag="LGJ20_003515"
     CDS             complement(12895..14214)
                     /gene="uhpC"
                     /locus_tag="LGJ20_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBK2387529.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(14224..15726)
                     /gene="uhpB"
                     /locus_tag="LGJ20_003516"
     CDS             complement(14224..15726)
                     /gene="uhpB"
                     /locus_tag="LGJ20_003516"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBK2387530.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(15726..16316)
                     /gene="uhpA"
                     /locus_tag="LGJ20_003517"
     CDS             complement(15726..16316)
                     /gene="uhpA"
                     /locus_tag="LGJ20_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBK2387531.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(16391..16681)
                     /gene="ilvN"
                     /locus_tag="LGJ20_003518"
     CDS             complement(16391..16681)
                     /gene="ilvN"
                     /locus_tag="LGJ20_003518"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBK2387532.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(16685..18373)
                     /gene="ilvB"
                     /locus_tag="LGJ20_003519"
     CDS             complement(16685..18373)
                     /gene="ilvB"
                     /locus_tag="LGJ20_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBK2387533.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(18479..18577)
                     /gene="ivbL"
                     /locus_tag="LGJ20_003520"
     CDS             complement(18479..18577)
                     /gene="ivbL"
                     /locus_tag="LGJ20_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBK2387534.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
BASE COUNT         4913 a         5108 c         4619 g         4397 t
ORIGIN      
        1 tcacgcacat cacggggggc agcatagacc accatttcat tatcctgctg ttcaacctcg
       61 aacacctgaa gcgggtgaat caaattgagg ccaggttgaa tcaaccagtt tccatcgcta
      121 attttcattg ccgttcctta gttctgcact tcttgagagg tgaattgttg ctggcaacgg
      181 cgtttaccct gagccagctg ttccataacg gttttcagat tgtgcgtcgt gagtgagtag
      241 tagcgtttag cgataatcgc gctcagcaaa taacagatcg ccggaacaat cgtgaatagc
      301 gcaataatga tgctaatcgt ggcgctgttc tgcgcttttt ctgccgcatc atatccgcca
      361 taagccagca tccagccgat aagagcgccg ccgaaggcca accccagttt gagcacaaac
      421 agcgtgccag caaaactgat cccggtcagg cgtttaccat tgcaccactc gccgtagtcg
      481 acggtatcgg acatcattac ccactggata ggtgtcacca gttgatgcaa cacaccaatc
      541 acgaagatga agacaaacat agtgatgctg gcctgcatgg gaacaaagaa catcgcgagg
      601 ctaatcactg ccagcagggc gttcgtccac cagaagatag tgactttaca tttccagtcg
      661 gtcagaggtt ttgccagtgc ggaaccaatc aggttaccca cgcaataagt ggtgagaaaa
      721 gcgacaaaca cttccggcgt gcccaaaatc catgtgacgt aatacatcat cgccccaccg
      781 cgtacgcaca ccgccaggat attgaaaatg gttagtaaac cgacaatccg ccactggtcg
      841 ttttgccaga tatcacgtaa atcttcccgc atagacgttg ttgtaggtgg tgcttcaacg
      901 cgttctttag tggtgaagaa acaaaatgcc agcatcatga atgccaccac ggaaaggacc
      961 gcgataccgc cctggaaacc gagtggttta ttatcaccgc caattaaatt aaccagtggc
     1021 atcatcagaa cagtagaaag catgcctccc gcggtcgcca gcacaaaacg ccaggattgc
     1081 agcgagatac gctgagtcgg gtcattggtg attacaccac ccaatgcgca gtaagggata
     1141 ttgacgacgg tataaagtaa ggtaagtagg gtgtaagtaa ttgctgcata gatcattttg
     1201 ccgttcatac tgagatctgg cgtgctatag gccagtacac agacgatccc gaatggcagt
     1261 gcgccaaaca gtacccacgg acgaaattta ccccagcgag agcgcgttcg atcggccaac
     1321 aaccccatgc aaggatcgga aatcgcatcc agtgcacgag cgaccaaaaa catggttccg
     1381 acaaatccgg caggaatgcc aaaaatatcg gtataaaaga acatcatata taacattacg
     1441 ttatcgaaaa taatgtggct ggcggcgtct cccatgccat aaccaatttt ctctttaacg
     1501 gacaacactt cactcttcat ctgcttttcc ttgatgctat aggggtgctg agaccgataa
     1561 cgcattttgt aaaccatccc tgtgagagcg aatattccga tatctggtta ttaatttatg
     1621 attcttgttt tatgtgatcg tggtagcgtt aattccgctc atatatcatt gtaaaatatg
     1681 ggttttatat gaactataat gctttcgtga taatacgctg cgtgtattag gcggaaaaaa
     1741 ctgatctggg ggatgtagaa actcaaggaa gtagctataa tgcgccccgc ctccatgtag
     1801 caatcgaggc gcggaagatc gtcgtctccg gtgaggcggc tggacttcaa atccagttgg
     1861 ggccgccagc ggtcccgggc aggttcgact cctgtgatct tccgccaaaa tgcctctcct
     1921 gacgttctcc aaccccttgt aatactgtca atccagatat tttctactgc tcaatattca
     1981 atcggcttca ggaatacctg gtctaaggtt ttgtgatgga cgatgagcgg ctgaagaatc
     2041 cgcctgtcgg ttcttctgtc ctcgaacgca tccgcgatat tcacgccagt atgcgccgtg
     2101 tttatttgca ggtgatttgc ccccctgctg actctcagcc aactgtatcg ccattaaggt
     2161 gaagatgttg ttttactcgc cccagaacac ccttttttat acgactattc tatttcttta
     2221 accgctggcg aaaaaaactg actataaact tcaccaataa tcagactgcc atcaagatta
     2281 atttgttcat ttaatatatc aaatcaactt gttttatttt tactatcaaa aatagctcat
     2341 tgccttacta taattaatat ataaattcgg taattaattc ttaacatgct tttactaata
     2401 atctcaattg ctggccctat aatattgcgc tagcattgct ttctggttgt atcagcgata
     2461 ctcaaaaatt cttaatacaa tactcattcg actggtactt atttgtaact cagttatatt
     2521 ttttcgcccg gtgattcaga agaatgcaaa aaacggctac cactccatca aaaatacttg
     2581 atctcactgc cgcggcattt ttacttgtcg cctttctgac gggtattgcg ggcgctcttc
     2641 agactcctac cctaagtata ttcctcgcag atgaactgaa agcccgtcct ataatggtag
     2701 gttttttctt caccggtagc gctattatgg gaattctggt cagtcaattt ctggcaaggc
     2761 actccgataa acaaggcgac cgtaaattac tgattctgct atgttgctta tttggagtgc
     2821 tggcctgcac gctttttgcg tggaatcgca actacttcat tctcctctca acgggcgtac
     2881 ttctgagtag ttttgcttcc accgcaaacc cgcaaatgtt cgccctcgcc cgtgaacacg
     2941 ccgacagaac aggccgtgag acggtcatgt tcagtacatt tttacgtgct cagatctcgc
     3001 ttgcctgggt tatcgggcca ccgctcgctt atgaactggc aatgggattt agttttaaag
     3061 tgatgtatct caccgcagcc atcgcatttg ttgtttgcgg actgatagtc tggttgtttt
     3121 tgccatcaat acaaagaaat attcctgtcg ttacccaacc cgtagaaatt ttaccctcca
     3181 cccacaggaa gcgggatacg cggctacttt ttgtggtctg ttcaatgatg tgggcggcga
     3241 ataatctcta catgataaat atgccgctat ttattattga tgaactgcat ctaaccgata
     3301 aactggctgg agaaatgatt ggtatcgctg ccggtctgga aattccgatg atgttaatcg
     3361 caggctatta catgaaacgt attggcaagc gactattaat gctcattgct atcgtgagtg
     3421 gaatgtgttt ttacgccagc gtactcatgg cgacgactcc ggcggttgag ctggaattgc
     3481 aaattcttaa tgccatcttc cttggtattc tctgtggtat cggcatgctt tattttcagg
     3541 acttgatgcc tgaaaaaata ggctctgcga caacgttata tgcaaatact tcacgcgtcg
     3601 gctggattat cgccggctct gttgacggaa ttatggttga aatctggagc taccatgcgt
     3661 tgttctggct ggcgataggg atgttgggta ttgcgatgat ttgcctgctg tttattaaag
     3721 atatttagct caccagtctg ggctaagaaa aatcggcgtg gcgcaaacag agccatcctc
     3781 gccatttttg ctatcatgcc tgcatacata aacgacaaaa cagtatgcag agggaaaaat
     3841 gggttccacc agaaagggga tgctgaacgt tctgattgcc gccgtgttgt ggggaagttc
     3901 aggggtctgc gcgcaataca tcatggagca aagccagatg tcgtcgcagt ttttgactat
     3961 gacgcgtttg atattcgccg gtttgattct actgacgctg tcatttgttc atggcgataa
     4021 aatcttttct attattaaca atcataaaga tgccattagc ctgctgattt tttccgtggt
     4081 tggcgcgcta actgtacagc tcactttttt gctaaccatc gaaaaatcga acgcagccac
     4141 ggcaacggtg ctgcaattcc tctcaccgac gattatcgtc gcctggttct cactggtgcg
     4201 taaatcgcgc ccgggcattc tggttttctg cgctattttg acatcgctgg tcgggacttt
     4261 tttattggtg acacacggta atccgacgtc attatcgatc tctcctgccg cgttgttctg
     4321 gggcattgcc tcggcatttg ctgctgcatt ctataccacc tatccctcaa cgctaattgc
     4381 ccgctatggc acgttaccag tcgtcggctg gagtatgctg attggcggtc tgattctgtt
     4441 gcctttttat gccagacaag gaacaaactt tgtcgttaac ggcagtttga ttctggcgtt
     4501 tttttatttg gtggtcattg gtacgtccct gacatttagt ctgtacctga aaggagcaca
     4561 attaattggc ggtccaaaag ccagcatttt gagctgtgca gaaccattaa gtagcgcgct
     4621 actctctttg ctgttgctgg ggatcacgtt cacattaccg gactggctgg gaacgctgct
     4681 gattctgtca tcggtgattt tgatttcaat ggattcccgt cgccgcgcca gaaaaataaa
     4741 tcgtccggcg cggcatgagt gagaattacc agccaggcac cgcgccaccg ttaaaaatgg
     4801 tttctgccgc tttagcgact tcgggtgatt gataagattg cagaaattcc ttcacgtttt
     4861 ctgcattctt attatcttcc cgtgccacca aaatattcac atacggcgaa ttcttatctt
     4921 caataaatac gctgtcgtgc accggagaaa gccctgtctg ctgaatgtaa gtggtgctga
     4981 taatcgctac atcaactttg ggatcatcca gtacgcgcgg gagctgcgcc ccctcgagtt
     5041 ccataatctg caaatgacga ggattatcgg ttatatccag cgcggtaggt aataaccctt
     5101 ttccctcttt cagagtaatt agcttctctt tttgcaacag taaaagtgcg cgaccaaggt
     5161 tggtcgggtc gttggggatc gccactgtcg cgccttcttt tatttgcgcc acggttttga
     5221 tttttttgga ataacccgcc atagggaaga caaacgtatt tcccaccgct accagtttat
     5281 aaccatgcgc ctgattatct tgttcaagaa agggtcgatg ctggaagaca ttcgcatcaa
     5341 gttcgccatg atttgtcgca tcgttcggta gcaacgaacc gctaaatcct accagttcaa
     5401 catcgaggcc atatttctct tttgccacct ttttagcgac ttccgcgaca tcttgttctg
     5461 cgccatttat aacgccaact ttaatgtgtt ttgcatcgct gctactctgg tcgcaacctg
     5521 ccagcagaat tccggccagc aataatgcgg cccctgtccg tagatgatgt gttgtcagtt
     5581 tcattttttt atccttttat taatgattac ctgactatag cggtaaggtc gctgcggttt
     5641 aaaaaacgaa acgctatcga taagaataaa aaggaataaa agtggaatat aaagtctggc
     5701 acttcttact gaccacgcaa gcgcgtttcg tacagcatga tgagagcgat gaaagcaaac
     5761 tacacttatg cttcattcga tacacatttg taaaaggttg aatccatgaa accaacgaca
     5821 ctactgctta ttttcacttt tttcgctatg ccaggcatcg tatacgccga gtcgcctttt
     5881 agctcactgc agtccgcaaa agagaaaacc acggtattac aagatttacg taaaatttgc
     5941 acgccacagg cgtcattatc agatgaagcg tgggaaaagt taatgttgtc agatgaaaac
     6001 aataaacaac atatacgcga ggccatcgtg gcgatggaac gtaataatca gagcaattat
     6061 tgggaagcgt tgggtaaggt agaatgcccg gatatgtaag aacagcaaca actactgacc
     6121 aacgcttctt tacctgatat caggatttct tcattttcac ctttgcagta accaacaatg
     6181 cagtcagcaa catcaatgtg ccggacaaca tcaacggcga agtcagacca atattatcca
     6241 gcgcataacc gccgattgcc gcgccacagg tattagcaag ctgaataacc gccacctgaa
     6301 tagacccggc tttttctgcc tgatcggcca gcgagcgggt gatccacgtt gaccagccga
     6361 cgggaaccaa tgcaaaagtt agcccccaga taatcgccac gccggtagca acgattttat
     6421 cgcttcccca caacgtcagc accaacgcac tcacagccag tattaacggc gcgcctgcta
     6481 aggccagttt taccgaacgt ttaagaatga acgacgaaag cgacgtacca ataaagctgg
     6541 cgataccaaa actcaacagc accagcgtta agccatccac gccgaatccc gccaggttca
     6601 tatacaccgg gcgaatatac gtgaagaaag caaactgccc ggcgaaagac atgaagatgg
     6661 cgatcatccc tgccatcaca cccggacgtt gtaataagcg gaaagtattt tgtttctgat
     6721 gcgagggttc gcctggcagt gaaggcaatg atttgataat ccagaaaata cacagcacgc
     6781 ccatcaccgc cgccgcatta aagacattgc gccaaccgat aagctcgcct aaaaaactgc
     6841 ccaacggcgc ggcaatcacc agcgcaatag aaaccgcgcc gaagatcacc gacagtgcct
     6901 tcggcaccgt acgcggcggc accagacgca tggtcagcga cgccgacatc gcccagaacc
     6961 cgcccagcgc cagccccaga caggcacgac cgattaaaag caaactgaat gagttagcaa
     7021 aggaaaccag caagcaggag agcgtcagca aaacggcaaa caaaataaca acgtagcggc
     7081 ggtcagtagc ctgaattgtc tgggtaataa acaaactggc aaacattgcc acaaaggcgg
     7141 tcacggtcac cgattgcccg gcaacccctt ccgaaatgcc taaatcctgg gccattggcg
     7201 tcaacaaact gacgggcaaa aactcaacga taatcagaca ggcgacacaa aacgccaccg
     7261 agaaaacggc tgaccagttc ggtcgggtga tggcatccgc gccgcggttt tcggcaataa
     7321 attcactcat ggtgttaccc gtggcatgtt tcaggggaaa agccgaccac tttaacattg
     7381 aaatgtgtga cgcatttaac gtttttgcaa cttttacggt gttgcttgag ctggagttaa
     7441 gatcgaaacg gaacagggca aagattaccg gatgcggcgt gaacgtctta tccacccgac
     7501 aggtcaggcg cggtctgtag gctgataaga cgaaacggtt tcaatgcaac cagtgaacgc
     7561 cctcggcagg ctgaaagaac gcgttatagc gcatctgaaa agcattaata tcctgcattt
     7621 caggttcggt ttcgcgcaga aaacctagcg ccagcctgac ctgagcgtcg gtaatcggcg
     7681 cggcgaggcg agctttttgt aaaatccgat gccattgcag caggttctgt tcgctaccat
     7741 cgacaatgac aatttgccag atgagcaaca cctgcgcggc gttttgcagg tgcgactcat
     7801 ccggacgatg caaataacgt aaaaattcgt caccagctga ctttcccatt tgatcgacca
     7861 tcgactccac gggtacgaca tcgatgttca gccgatcgct gagacggcga atcgcttttc
     7921 tggccccgga tgtcagcacc cgaaaaccaa ccacaaacac taccgccagc gtggcgagca
     7981 ttatccagat catgagttct cctgcgtaat cagaactcat gatatctgga aaccctcgcc
     8041 agttactaat ccaccagaat aatcttcagt gcaaacagag ctgcgaccac caccacacac
     8101 aggttcagat cgcgccagcg cccggtgcat actttcatga tgcagtacga cataaagccg
     8161 agtgcaatcc cttcggtgat cgagaaagta aagggcatca tcaccgtggt aataaacgca
     8221 ggcaccgatt cggtaaaatc atcccagtta acgcgcgcca ggctcgaagt catcagcacg
     8281 ccaacaaaga ttaacgctcc ggcggttgcg taaggaggaa ccatcgccac cagcggtgag
     8341 aagaacataa ctaacaggaa cataacgcca accacaaccg cagtcagccc cgtgcgacca
     8401 ccgactgcca caccagaagt actttcaata taggcagtaa cagacgaggt gccgataaac
     8461 gcccccgcca ccgaactgac gctatcaaca tacagcgcct tattcatatt ggggaattta
     8521 ccgttactat ctattaagcc cgctttatca gttacaccaa ttaatgttcc tgatgaatca
     8581 aataggttga ttagcataaa ggagaaaatg ataccggcga gttcaagtgt taacgcgccg
     8641 ctcaaatcta cttcaccaat gacgccgcta atatcaggcg gaatggaata gacgccgcta
     8701 aaatgaacat caccgaaaaa taatccacag caagacgtca ccacaataga aaccagcacc
     8761 gcggcatgaa aatgacgtga tgacaacacg gttataataa aaaaccctaa aatacctaac
     8821 aacacgccgt gagaacttaa atcgccaatc atcaccagcg tgtctttatt ggcgacaata
     8881 acgccagtat tttttaatcc cattaaggcg ataaataatc caattccgct ggtgatacca
     8941 atacgtaaac ttaagggaat gttggagatc atccagtacc ggatacgaaa cagcgtgagc
     9001 aaaaatagtc caactgcgcc ccagaatatt gcgcccatcc cggtctgcca ggagatgccc
     9061 atcgccccca cgaccacgaa ggcaaagaag gcgttcagcc ccattgccgg agccagcgcc
     9121 acgggtaagt tagcaaatat ccccatcgca atactgccga taccggcaat caaacaggtg
     9181 gtaacaaaca ccactttcgg gtccatttgt gccgcgccga ggatttgcgg gttcacaaaa
     9241 acgatgtaca ccatggttaa aaaagtggtc atccccgcaa tcaattctgt gcggacggtg
     9301 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
     9361 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
     9421 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
     9481 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
     9541 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
     9601 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
     9661 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
     9721 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
     9781 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
     9841 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
     9901 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
     9961 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
    10021 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
    10081 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
    10141 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
    10201 cgggtttggg tggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
    10261 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
    10321 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
    10381 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
    10441 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
    10501 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
    10561 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
    10621 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
    10681 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
    10741 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
    10801 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
    10861 atgtgagctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
    10921 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
    10981 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
    11041 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
    11101 tgcccattgc cggcctgatg agcaccgaca cggcgcagtt gctggcggaa caaattgacg
    11161 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
    11221 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
    11281 gcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
    11341 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
    11401 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
    11461 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
    11521 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
    11581 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
    11641 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
    11701 agatatattc gttgctggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
    11761 cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca
    11821 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
    11881 agagtttcag ttcctggaac gcgtacacgg ttgaccactg gtcgatacca atacgcacca
    11941 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
    12001 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
    12061 tctcctcgcc gaacagctct tccgctttgc cgaggccata agattccggg gagtcgctgc
    12121 cgtaacgcag gccgataaaa ccgacaatca acgcgataat cgacgggaag ataaacatgc
    12181 cgatcacgtg gccgtcgaac aggtaatttg cgccgaacag cgccacacca gccgcacccg
    12241 cgccgccaag gttatgggaa atgttccaga aaccgaggaa ggtcccgcgc ttacgacgcg
    12301 gtgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
    12361 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
    12421 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
    12481 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
    12541 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
    12601 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
    12661 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
    12721 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
    12781 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
    12841 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
    12901 ttcgcgcggt gcctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
    12961 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
    13021 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
    13081 tgccgcctct ttgtgggaac actctgccgc cgccatgccg attaacatct gcgggccaaa
    13141 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
    13201 cagccacagc gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
    13261 acggttgccg ttaaacaatt tgtccgagcc ccaaccggct accagcgcac cgataaatcc
    13321 gcccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagtgtctc
    13381 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
    13441 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtca ggatctcttt
    13501 acgcgtcaac cctgcccctt cttgttgttg agcaatttcc agcgcgtcgt gccgccattc
    13561 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgt agccgccagc agagaaaaat
    13621 ccccacgact atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgcag
    13681 cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
    13741 taatgcccac caaccgccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
    13801 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagcacgg caaacgccca
    13861 tagcgacgtc gagaagccaa acagaatgtt catgatgccc gtggcgataa gccctatccc
    13921 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
    13981 gtaagtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
    14041 agcaaggatt tctggtaccg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
    14101 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
    14161 atatttatca gtcattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
    14221 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
    14281 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
    14341 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
    14401 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
    14461 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
    14521 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
    14581 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
    14641 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gctgcccgct
    14701 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
    14761 ggctgtgatg gtctgaccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
    14821 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
    14881 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
    14941 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
    15001 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
    15061 cagcgcgata atcggcagcg ccagacaaaa tggcgtaaag cgcgacagtt cgtccggcaa
    15121 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
    15181 gcgccagttg atcggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
    15241 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
    15301 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
    15361 cgccgccgtt aacgccgcac cctgtaacag caaggtgcgc cagtcacgct gatggcgata
    15421 gcgcgagatc agcgccacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
    15481 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cgcccagcag
    15541 caccggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
    15601 taacagcacc gccatatcag ggcgctcaac caggtgcagg ctgatactcc acaggcaaaa
    15661 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
    15721 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacacccagt
    15781 ttttccatca ggttggcgcg atggacgtgc accgttttcg gcgacaagcc cagttcggca
    15841 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctgccg ttcgcgtttg
    15901 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
    15961 ccgcccgtgg caaccgtatg caccgccgca atcagttcgt cagggctaca gcgtttggag
    16021 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgccggact gtcatgaaca
    16081 gagagcatta ttgtcgccat acctttcggc agctggctta gcagttccag accggagata
    16141 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
    16201 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
    16261 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
    16321 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
    16381 ccttgagcgg ttactgaaaa aacaccgcaa tcttgttaaa catagtcgga tcggactgat
    16441 tacgctgcac tttcaccaca tcttccagct tatcgatttg gcttatcatc tgctccagac
    16501 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
    16561 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
    16621 cgcccggatg gttgcgaacg gtgagctcca gaatcacgtt gtcatgagtt gtgttttgca
    16681 tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
    16741 tttcttcggc atcaatgcgc acatggatca gcgccgggcc agggcgattg atgatttcct
    16801 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
    16861 cggcaatctg cataaagttg atttttcccg gataggtggc ggcaaaaacg ccttgctcgt
    16921 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
    16981 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
    17041 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
    17101 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
    17161 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
    17221 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
    17281 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
    17341 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
    17401 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
    17461 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
    17521 gcgcaccgag cactatcaac aaatccgcct cctgcaaaat atagttggtg ctgcgcacgc
    17581 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcaacatg cccagcgcca
    17641 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgtgcgggcg
    17701 cattgatcac accgccgccc agataaagca ccgggcgttt ggcagcgtta atcatcgccg
    17761 ctgcgtcacg aatgctttct tcgctaaagg cgggggcggc ggctttttct gccatagcgg
    17821 gctgtgtttc aatctcaaaa actgccgttt gcacatcctt aggaatgtct atccacaccg
    17881 ggcctgggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
    17941 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcca
    18001 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccggtg atgcaaatca
    18061 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
    18121 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccatcccc tgagcgataa
    18181 atcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
    18241 catcgtaaac aggcaggata gaaccgcccg gaatgcccgt cacaatctta atgccctgct
    18301 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc gatgttgtgc
    18361 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
    18421 gaaaaacccc gcccggtttg cgccggcggg gttttggaat cgtgtgttgt tccagtccct
    18481 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatagcgcag
    18541 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
    18601 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
    18661 ggaaaattga ggggctgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
    18721 gagcgaggtt tcatcagccg cctgcggctg gtaaccgcaa agcacactgt attatgtcaa
    18781 cactgaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
    18841 tagggcatag ttgagtatcg atcacagttt gcgttttgtc caaatattac tgtttattta
    18901 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
    18961 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
    19021 cacactgtgt tatgtca
//
LOCUS       DAFATA010000085        18874 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFATA010000085 DAFATA010000000
VERSION     DAFATA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.086"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            132..1040
                     /gene="melR"
                     /locus_tag="LGJ20_003521"
     CDS             132..1040
                     /gene="melR"
                     /locus_tag="LGJ20_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBK2387535.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1239..3506
                     /gene="adiA"
                     /locus_tag="LGJ20_003522"
     CDS             1239..3506
                     /gene="adiA"
                     /locus_tag="LGJ20_003522"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBK2387536.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            3831..4592
                     /gene="adiY"
                     /locus_tag="LGJ20_003523"
     CDS             3831..4592
                     /gene="adiY"
                     /locus_tag="LGJ20_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBK2387537.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            4729..6066
                     /gene="adiC"
                     /locus_tag="LGJ20_003524"
     CDS             4729..6066
                     /gene="adiC"
                     /locus_tag="LGJ20_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBK2387538.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            6170..7813
                     /gene="eptA"
                     /locus_tag="LGJ20_003525"
     CDS             6170..7813
                     /gene="eptA"
                     /locus_tag="LGJ20_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBK2387539.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            7810..8478
                     /gene="basR"
                     /locus_tag="LGJ20_003526"
     CDS             7810..8478
                     /gene="basR"
                     /locus_tag="LGJ20_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBK2387540.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            8479..9579
                     /gene="pmrB"
                     /locus_tag="LGJ20_003527"
     CDS             8479..9579
                     /gene="pmrB"
                     /locus_tag="LGJ20_003527"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBK2387541.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(9555..9644)
                     /gene="pmrR"
                     /locus_tag="LGJ20_003528"
     CDS             complement(9555..9644)
                     /gene="pmrR"
                     /locus_tag="LGJ20_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBK2387542.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(9756..11258)
                     /gene="proP"
                     /locus_tag="LGJ20_003529"
     CDS             complement(9756..11258)
                     /gene="proP"
                     /locus_tag="LGJ20_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBK2387543.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(11522..12400)
                     /locus_tag="LGJ20_003530"
     CDS             complement(11522..12400)
                     /locus_tag="LGJ20_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBK2387544.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(12397..14625)
                     /gene="crfC"
                     /locus_tag="LGJ20_003531"
     CDS             complement(12397..14625)
                     /gene="crfC"
                     /locus_tag="LGJ20_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBK2387545.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            15027..15362
                     /locus_tag="LGJ20_003532"
     CDS             15027..15362
                     /locus_tag="LGJ20_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBK2387546.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            15522..15965
                     /gene="yjdN"
                     /locus_tag="LGJ20_003533"
     CDS             15522..15965
                     /gene="yjdN"
                     /locus_tag="LGJ20_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBK2387547.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            16098..16886
                     /gene="phnC"
                     /locus_tag="LGJ20_003534"
     CDS             16098..16886
                     /gene="phnC"
                     /locus_tag="LGJ20_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBK2387548.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            16911..17927
                     /gene="phnD"
                     /locus_tag="LGJ20_003535"
     CDS             16911..17927
                     /gene="phnD"
                     /locus_tag="LGJ20_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2387549.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            18052..18831
                     /gene="phnE"
                     /locus_tag="LGJ20_003536"
     CDS             18052..18831
                     /gene="phnE"
                     /locus_tag="LGJ20_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBK2387550.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
BASE COUNT         4752 a         5031 c         4641 g         4450 t
ORIGIN      
        1 agttttcggc ctcatgatgc tcggacgctc ccctcgcctc tctgggaaga gggtgaaaac
       61 cgtgctccca ctcgcagtca tcctccctca ctcctgccat aattctgata ttccaggaaa
      121 gagagccatc catgaataca gatacgttta tgtgcagcag cgacgaaaaa cagacccgca
      181 gcccgctgtc gctgtattcg gaataccagc ggatggagat tgagtttcgc gcaccgcata
      241 tcatgcccac cagccactgg catggtcagg tcgaagtgaa tgtgcctttc gatggcgatg
      301 tggaatacct gatcaacaat gaaaaagtga atatcaatca gggtcatatc acgctgttct
      361 gggcctgtac accgcaccaa ctaacagata ccggaacctg tcagagcatg gcgattttta
      421 atctgccgat gcatctgttt ctctcctggc cgctggataa agacctgatt aaccacgtca
      481 ctcacggcat ggtgatcaaa tcactggcca cacagcaact tagcccgttt gaagtgcgcc
      541 gctggcagca ggaattaaac agtccgaacg agcaaattcg ccagctcgcc attgatgaaa
      601 ttggcctgat gctcaagcga tttagcctct ctggctggga accgattctg gtcaataaaa
      661 cctcgcgcac acacaaaaac agcgtctcgc gccatgcgca attttatgtt agccagatgc
      721 tgggctttat tgccgaaaac tatgatcagg cgctgaccat caacgatgtg gctgagcacg
      781 tcaaacttaa cgccaactat gcaatgggga tattccagcg ggtcatgcaa ttgacgatga
      841 aacagtacat taccgcgatg cgcatcaacc acgttcgcgc gttactgagc gataccgata
      901 aaagtattct cgatattgcc ctgacggcag gctttcgttc aagtagtcgt ttttacagca
      961 cgttcggcaa atatgtcggc atgtcgccgc aacaataccg caaacttagc caacagcgcc
     1021 gccagacgtt tcccggctaa agacgatatc agtatcagcc aaaaaaatag tttagccgtc
     1081 gataataagc agtgaaaata atttaaaata atcacataac gcattgttta taaaaataaa
     1141 tatttcaaaa atgtttgttt ttcacgcgct ttacagcccg aaaaggccgg aagatacttg
     1201 cccgcaacga agattccttc ataaccgggt aagcaatgat gaaagtatta attgttgaaa
     1261 gcgagtttct ccatcaagac acctgggtcg gtaacgccgt tgagcgtctg gcagatgctt
     1321 taagccagca aaatgttacc gtgattaaat ccacctcctt tgatgatggt tttgccattc
     1381 tctcttcaaa cgaagccatt gactgcctga tgttcagtta tcaaatggaa catccggacg
     1441 aacatcaaaa cgtcagacaa ttgatcggta agcttcatga gcgccaacaa aacgtgccgg
     1501 tcttcctgtt gggcgatcgg gaaaaagccc tcgccgcaat ggatcgcgac ctgctggagc
     1561 ttgtcgatga attcgcctgg attctggaag ataccgccga ctttatcgcc ggacgcgctg
     1621 ttgccgcgat gacccgctac cgccagcagc tgttgccgcc actgttcagc gcgctgatga
     1681 aatatagcga catccatgaa tattcctggg cagcgccagg ccaccagggc ggcgttggtt
     1741 ttaccaaaac acccgccgga cgtttctacc atgactacta tggagaaaat ctgttccgca
     1801 ccgatatggg catcgaacga acttccctcg gttctttgct tgaccatact ggcgcatttg
     1861 gcgaaagcga aaaatatgcc gcacgcgtat ttggtgccga tcgctcctgg tcggtagtcg
     1921 tcggtacttc cggctctaac cgcaccatca tgcaggcttg catgaccgat aacgatgtcg
     1981 tggtcgttga ccgtaattgc cataaatcca tcgaacaagg cttgatgctg acaggcgcga
     2041 aaccagtcta catggtgcca agccgcaacc gctacggcat tatcgggcca atctatccgc
     2101 aggaaatgca acctgaaacc ttgcagaaga aaatcagtga aagcccgctg actaaagaca
     2161 aagctgggca aaaaccgtct tactgcgtgg tgaccaactg cacctatgac ggcgtgtgtt
     2221 ataacgctaa agaagcgcag gatctgctgg aaaaaacctc cgatcgtctg cactttgacg
     2281 aagcctggta cggctatgca cgtttcaacc cgatctatgc cgatcactat gccatgcgcg
     2341 gcgaacctgg cgatcacaac ggtcctaccg ttttcgccac ccactccacc cacaaactgc
     2401 tgaatgcgct ttcacaggct tcttatattc atgtacgtga aggtcgtggg gcgattaact
     2461 tctcccgctt taaccaggcc tacatgatgc atgccaccac ctccccgctg tatgccatct
     2521 gcgcatccaa cgacgtggcg gtgtcgatga tggacggcaa cagcggcctg tcactgacgc
     2581 aggaagtgat cgacgaagcg gttgatttcc gtcaggcgat ggcgcggctg tataaagagt
     2641 tcaccgctga cggtagctgg ttcttcaaac cgtggaacaa agaagtcgtc accgacccac
     2701 aaaccggcaa aacctatgac tttgctgacg caccaaccaa actgctgacc accgttcagg
     2761 actgctgggt aatgcatccg ggcgaaagct ggcacggctt caaagatatt ccggataact
     2821 ggagtatgct cgacccgatt aaagtcagca tccttgctcc gggaatgggt gaagatggtg
     2881 aactggaaga aaccggtgtt ccggcggcgc tggtcactgc ctggcttggt cgccacggca
     2941 ttgtgcctac ccgcaccact gacttccaaa ttatgttcct gttctctatg ggcgtaaccc
     3001 gtgggaaatg ggggactctg gttaacaccc tttgctcctt caaacgccac tatgacgcca
     3061 acacaccgct ggcgcaggtg atgccggaac ttgttgaaca atatcctgac acttacgcga
     3121 acatggggat tcacgatctg ggtgacacca tgtttgcctg gctgaaagaa aacaaccctg
     3181 gcgcacggtt gaacgaagcc tattccggcc tgccgatggc ggaaatcacc ccgcgtgaag
     3241 cgtacaacgc gattgtcgac aacaatgtcg aactggtatc cattgaaaat ctgccaggac
     3301 gcatcgcggc aaactcagtt atcccgtatc cgccaggaat cccgatgctg ctgtctggtg
     3361 aaaacttcgg cgataaaaac agtccgcaag taagttattt acgctcgctg caatcctggg
     3421 accaccattt ccctggattt gaacacgaaa ctgaagggac tgaaattatt gacggtattt
     3481 accacgttat gtgcgtgaaa gcgtaaccac tattccgctg aaggcgtaat tgtttaaata
     3541 acattacgcc gcctggcctt aggccttttg agtatggcaa cgttttcata aaaattgctg
     3601 caaacaaaaa tgtcatactt tttgcgcggc cccaccccgc gctttttttt gcctgttatt
     3661 tatcctgtaa aaaaatatgt acatgagaaa ttactataaa aatttgtaat attagtaaaa
     3721 ctcgttattt ttatgcatgt ttatattcat catacaatta tataaccatt tcccggtatc
     3781 gctttgcttt agcgagaact ggtcttttat ttgcactcag gagtacatgt atgaggattt
     3841 gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata agggtgaatg
     3901 ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt gaaaataata
     3961 ttatcgacgt ctcctctctt aacaacactt tggttgctca tattagtcac gacatcatca
     4021 aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg caacgtagcg
     4081 ctacgcccat actcaccctg ccatgcctga cgccagacgt ctttcgcgtt gccgcgcaac
     4141 acagcatgat gcccgcagaa actgagtcag aaaaggaacg aacacgcgca ttattattca
     4201 cggtgctatc ccgttttctc gacagtaaaa aattcctttc attaatgatg tatatgttac
     4261 gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatattcac aaagactgga
     4321 atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag aaaaagttga
     4381 aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa
     4441 atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc ggctacaaca
     4501 gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgccg ctgcattatg
     4561 ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag agctatatta
     4621 ataacggcat cagcgataac ccggtcgata ataattcaac tatcgaatgc aggcgtatga
     4681 tatgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat gtcttcggat
     4741 gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg gaatattatg
     4801 gggtcaggtg tttttctgtt acctgcaaac ctggcctcta ctggcgggat tgccatttat
     4861 ggatggttgg tgacgattat cggtgcactg gggctctcga tggtatacgc caaaatgtcg
     4921 ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt tggcccgttt
     4981 ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa tatcgccatg
     5041 gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga tccattggta
     5101 ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg
     5161 aaaatgatca cccgtgtgca ggcagttgcc accgtgctgg cgctgatccc catcgtcggg
     5221 attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc atggaacgtc
     5281 agcggcctgg gcaccttcgg tgcaattcaa agtaccctta acgttacgct gtggtcgttc
     5341 atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa acgcaatgtc
     5401 cctatcgcca ccattggtgg ggtattgatt gccgccgttt gctatgtact ttctaccacc
     5461 gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcttcgcc attcggtgat
     5521 gccgcacgga tggcgttggg tgacaccgcc ggggccattg tttccttctg cgcagctgcg
     5581 ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc gaaagccgct
     5641 gccgatgacg gactgttccc accaattttt gcccgtgtaa ataaagcggg tacgccagtg
     5701 gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagcag catttcacca
     5761 aacgcgacca aagagttcgg tctggtttct tccgtctcgg tcatctttac actggtgcca
     5821 tatctttaca cctgtgcggc gttactgctg ctcggacacg gtcactttgg taaagcacgc
     5881 ccggcatatc tggcagttac taccattgcc ttcctctact gcatctgggc cgtggtgggg
     5941 tccggagcga aagaggttat gtggtcattt gtcaccctga tggtcatcac cgccatgtat
     6001 gcgctgaatt acaaccggct acataaaaac ccgtatccct tagatgcacc aataagcaaa
     6061 gattaattcc ccttaatcca gcaaacataa aagccaacct taagaactta aggttggctt
     6121 aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg
     6181 cctactaaaa agaccctctt tgaatttact cgcctggcta ttgttggccg ctttttatat
     6241 ctctatctgc ctgaatattg ccttttttaa acaggtgttg caggcgctgc cgctggattc
     6301 gctgcataac gtactggttt tcttgtcgat gccggtcgtc gctttcagcg tgattaatat
     6361 tgtcctgaca ctaagctctt tcttatggct taatcgacca ctggcctgcc tgtttattct
     6421 ggttggcgcg gctgcacaat atttcataat gacttacggc atcgtcatcg accgctcgat
     6481 gattgccaat attattgata ccactccggc agaaagttat gcgctgatga caccgcaaat
     6541 gttattaacg ctgggattca gcggcgtgct tgctgcgctg attgcctgct ggataaaaat
     6601 caaacctgcc acctcgcgtc tgcgcagtgt tcttttccgt ggagccaata ttctggtttc
     6661 tgtactactg attttgctgg tcgccgcact gttttataaa gactacgcct cgttgttccg
     6721 caataacaaa gagctggtga aatccttaag cccctctaac agcattgttg ccagctggtc
     6781 atggtactcc catcagcgac tggcaaatct accgctggtg cgaattggtg aagacgccca
     6841 ccgcaacccg ttaatgcaga acgaaaaacg taaaaatttg accatcctga ttgtcggcga
     6901 aacctcgcgg gcggagaact tctccctcaa cggctacccg cgtgaaacta acccgcggct
     6961 ggcgaaagat aacgtggtct atttccctaa taccgcatct tgcggcacgg caacggcagt
     7021 ttcagtaccg tgcatgttct cggatatgcc gcgtgagcat tacaaagaag agctggcaca
     7081 gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc tgtggaatga
     7141 caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct
     7201 gaatctgcct ggtcagtgca tcaacggcga atgctatgac gaagtactgt tccacgggct
     7261 ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca ccatcggcag
     7321 ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta ccccaacctg
     7381 cgacaccaat gagatccaga cctgtagcaa agagcaactg gtgaacactt acgacaacac
     7441 gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag aacatcagga
     7501 taaatttacc accagcctgg tttatctttc tgaccacggt gaatcgttag gtgaaaatgg
     7561 catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac aggtgccgat
     7621 gctgctgtgg ctgtcggagg attatcaaaa acggtatcag gttgaccaga actgcctgca
     7681 aaaacaggcg caaacgcaac actattcaca agacaattta ttctccacgc tattgggatt
     7741 aactggcgtt gagacgaagt attaccaggc tgcggatgat attctgcaaa cttgcaggag
     7801 agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca gggattgatt
     7861 ctggcggcgc aaactgaagg ctacgcgtgc gatggcgtga caaccgcgcg gatggcggaa
     7921 caaagccttg aggccggtca ttacagcctg gtggtactgg atttagggtt acccgacgaa
     7981 gatggactgc attttctcgc ccgtatccgg cagaaaaaat ataccctgcc ggtactgatc
     8041 ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg tgccgatgac
     8101 tatctggtga agccttttgc gctggaagag ttacacgccc gtatccgcgc cctgctacga
     8161 cgccataata atcagggcga aagtgagctg attgttggca atctgacgct gaatatgggt
     8221 cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga atatgctctg
     8281 ctgtcacggt taatgctcaa agcaggcagt ccggtgcatc gggaaattct ctacaacgac
     8341 atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat ccacaatctg
     8401 cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta tatgctggtc
     8461 gcgaatgagg aaaactaatt gaatctgatg cattttctgc gccgaccaat atcgctgcgc
     8521 caacggctga tattgaccat cggggccatt ttgttggtgt ttgagctgat cagcgtcttc
     8581 tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct acgcgacaat
     8641 cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag cctgattgtc
     8701 cccggcgtct ttatggtcag cctgacgcta tttatctgct atcaggcggt acgccgcatc
     8761 acccgcccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga caacttaacg
     8821 cccattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc gttgaacgat
     8881 ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tgtttaccgc tgacgtcgcg
     8941 cacgaactgc gaacgccact ggcgggggtg cgtttgcatc tggaactgct ggcgaaaacg
     9001 catcacattg atgtagcacc gttagtggca cggcttgatc agatgatgga gagcgtctcc
     9061 cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta tcaacatgta
     9121 aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat gctcgaccag
     9181 cgacagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca gggtgatgca
     9241 accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta cagcccgcaa
     9301 ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc agttgaagat
     9361 gaaggaccag gtattgatga gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg
     9421 gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat tacacagttg
     9481 catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg ggcctgggta
     9541 cggctgaaga aagatcagaa cgtggcaaac cagatataaa gaaagctgct gaccaccagc
     9601 acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact ctcctccgtt
     9661 aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc aattggcctg
     9721 ataagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg cggatgttgc
     9781 tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat attatcgatt
     9841 ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg tatatctgac
     9901 gccgccggtg tcgcaccttt caacggacga tttgccgtct ctttcatggt tacgccggtg
     9961 attaaaccaa tcaccgccac taccatcagg taataggcag gcatcatcag attctgcgag
    10021 ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac cgaaatatta
    10081 aatgccgccg ccagcgcgct gtaacggata tgcgtcggga acatcgctgg caaggtagaa
    10141 gccataacgc ccgtaaagca gttaaggatc accgccagca tcagtaaccc ggcaaaaatc
    10201 aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac aaacagggca
    10261 acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa gcccatcacc
    10321 ggctggacaa acagcatacc gatcataatg gcgataataa tcagcacccc gtggtcttcg
    10381 gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat gtagtaagtc
    10441 acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagtg tttggtggca
    10501 atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc ctgttccagt
    10561 ttatcgacat gctgctggaa cgccggagtt tcttccagcg catggcgcag gtaaagcccg
    10621 ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc gaggaagttc
    10681 gcttcgccga caatggtcga aattaacacc actacgccag cacccagcac aaacccggca
    10741 atagaaccga agtccagcca gctgcccata aagccacgtt tacggtcagg tgagtattcc
    10801 gcaacaaata tcgacgcccc ggtatattca ccgccgaccg agaaaccttg tgccatctta
    10861 cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca
    10921 atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt ctggcgacca
    10981 tatttatcgc ccaacatacc aaagaagagc ccgccaagcg gtcgaatcag aaagggaacg
    11041 gagaacgtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg gaaaaaaact
    11101 ttacctaatg cgtaagcaac aaaaccataa acaccaaaat caaaccattc cattgcatta
    11161 cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat ggtgacatca
    11221 cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc agagttggcc
    11281 catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc gggaattgca
    11341 ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt aaggaatgta
    11401 caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc taccagtgtt
    11461 taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg taactgatta
    11521 tttacgccaa atttacagag aagatttcct cgaccattgc ccccgccaga cgctgcgctg
    11581 aacagagtcg tggcatcccc agcgcgacag tttgccagcg ttgggtcact gaccagcaaa
    11641 cgggttgttt atctgccgtg gtccactttc ccagccaact taaaaaatca gcatgatccc
    11701 cgcccgtcgg aacggtcgaa gaggcaaacc actgatggta atagtgacgc gtggtcgggg
    11761 ccgcattgat actctctgcc agatgttgtg tcgctttcac gcgcaaatca cccagcaaca
    11821 acgccaccgc ttgcgggctt cccagacgac aggcatcgcc aaatgccccc caacgcaact
    11881 cttcgagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc ccagcacgcc
    11941 agcgggtaaa aatttgctcg ctctgatcac ctgcctgcac cagtaggcct cgttgcgtta
    12001 acgccttttc cttccagcgg caacgggctg aaaaccactc atacaaggcg gcaatttgct
    12061 gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc agcgccagat
    12121 gctgcgcgat ctgcgtcagc gccagatgac cgggatccag catcgacgcc agtttttcca
    12181 ccattgtcag tcgcaggcta agcggcgcat ttaacatgcc gccgttcgtc catgtctgaa
    12241 gctgcacgcg cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc tgatgggctt
    12301 gtggaaggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgggga taaaccgact
    12361 ccagcacgcg accggggccg tcaagtaacg tcttggtcaa tatcgttctg ccgtgaaaag
    12421 tgtttgaata tcatcgcgta acagctgggc gtcggtgtaa atccagccgt gagtcatcac
    12481 agtctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt gacgcagagc
    12541 cagactttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact cagcgaaaaa
    12601 agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt tagccatttg
    12661 ttggcaataa agatcgactt ctgcgcttaa agctcgttgc aacacactgt aatcaaccgt
    12721 ttctgtcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc agcgcgaaaa
    12781 agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcatcct ggcgcgaaat
    12841 gacttcactg aacagctggc gagtgttgaa gtggggaact acgccgtgaa aaacaggaaa
    12901 atgaaaccca ggacgaaacc ctgactcgct caattccatt ttgacttgtt gctcaatggg
    12961 ccgaataaca tcggttaaca cccggcaaag ggtggattca agctcggcaa aacgcagcgt
    13021 aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc aggtacgaat
    13081 ctcgcttaac ggctccgaat catcctgaaa caaggcggct aactgcgcat tcagcgcatc
    13141 ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc agctcgattt catgtttaac
    13201 ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt cgacctgatg
    13261 gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa aatccaggta
    13321 tccccgcgcc tgctgcgcgt aattcaacag tttatgcgca gcagatcgca gagcatacaa
    13381 cgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga acagcgaatc
    13441 ttcccacaac tgatcggcag catgacgaat atgttcgagg tccgccagat cgtcatgacg
    13501 ccagcgcctg ccgagcgcag catgggcaaa atcttccacc cagcgttgtt gctctggcgc
    13561 tggtaactta ccgttgttgg ctaactcatg gcgcgctcga ttcgccaggt agccccacat
    13621 cgacgacacc ggaaatatct gctgtggcgt aatacagcct ttcatcagcg tcccggaaat
    13681 cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc tgttgatcga acttattgac
    13741 cagcacatac agcggcaccg attgccccac cgccaaaatc gcctcacgga cctcttcatc
    13801 ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg cgcgtgccag
    13861 ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc cgggggtatc
    13921 cagtaacgtc aactgaccgg gataactctc cagccccgcc agatggacaa actccacttc
    13981 aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc
    14041 cttcgccagt cgcactaaat cattcaaact tttcagacaa tgaaaaatag gctgggcacc
    14101 cagataatat ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg cacgcatatc
    14161 tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca ggcgctgttg
    14221 taattgttga attaaacaat cgattggcgc gacatgtgaa aaatgcagta ccggttcctt
    14281 ttgcccgggc gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat tacgattagg
    14341 cagaacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt tcatggtacc
    14401 gacaattgcc agcaccattt ccagtcggga aattttacgc aactcattat tcagcatcgc
    14461 gtgacgttcg gcgatattag gctgactcca gggtaaagcc agttgtggcg cgtcgtctcc
    14521 gggtacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag
    14581 gcgttcagcc tcctgactta actcatacag ggtctgtgtg tacatagaaa attcttcctt
    14641 aaagcaaatt ttgttacttt tatttagcca gattgttttt gagttctgtt ttcggctttt
    14701 ataattactg caagaaataa ttttatattt agtgtgttgt tttttatcag aataaataac
    14761 gtcttctgat acgtttaaaa cgtcagaaag ataaaaatat catgtgaata aaaaaaagaa
    14821 caagtagagc attaacatta tcttaaataa taaatagagg caaaaagatt attttctttt
    14881 tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact
    14941 cagggatttt gtgtataatt gcggcctttt tcggcaatct gccgtttttt ggcgcttttg
    15001 ccctgctgac ttttgaggaa atccacatgt cattaccaca ctgcccaaaa tgcaactccg
    15061 aatacactta cgaagataac ggcatgtaca tctgcccgga atgtgcctac gaatggaacg
    15121 acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa agatgctaac ggcaatctgc
    15181 tggctgacgg cgacagcgtt accatcatta aagatctgaa ggtgaaaggt agctcttcga
    15241 tgctgaaaat tggcaccaaa gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg
    15301 attgcaaaat cgacggtttt ggtccgatga aactgaaatc tgagtttgtg aaaaagaact
    15361 gattgtattg tgatcggtaa gccggataag gcgctcgcgc cgcatccggc aacgatgccg
    15421 actgcctgat gcgacgctga ccgcgtctta tcaggcttcc ctctcatctg tataaatttc
    15481 gaactacact taactggctt ctcttaactg aggtcaccat catgccgtta agtccctacc
    15541 tctcttttgc cggtaactgt gccgacgcaa ttgcttatta tcaacgtacg ttgggcgctg
    15601 aactgctcta taaaatcagc ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga
    15661 actgcccttc cggaatgcaa tttcccgata ccgccatcgc tcatgccaac gtgcgcattg
    15721 ccggaagcga catcatgatg agcgatgcca tgccgtcagg aaaagccagc tactccggct
    15781 ttacgctggt gctcgattcg caacaggtcg aagaaggaaa acgctggttt gacaatcttg
    15841 ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat ggctttggca
    15901 aagtcaccga taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc
    15961 aataatccgc cgggaggccc gccctcccgc actgtcatcg aattcccgtt aactcttcat
    16021 ctgttagtca cttttaatta accaaatcgt cacaataatc cgccacgatg gagccacttt
    16081 tttagggagg ctgcatcatg caaacgatta tccgtgtcga gaagctcgcc aaaaccttca
    16141 atcagcatca ggcgctgcat gcggttgatc tgaacattca tcacggtgaa atggtggctc
    16201 tgcttgggcc gtcgggttcc ggcaaatcca cccttttacg tcacttaagc ggtttgatta
    16261 ccggcgataa atccgccggc agccatatcg agctgctggg ccgcacagtc cagcgcgaag
    16321 gccgtctggc gcgcgatatc cgcaaaagcc gcgccaacac cggctacatc ttccaacaat
    16381 tcaacctggt gaaccgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca
    16441 cgccgttctg gcgcacctgt tttagctggt tcaccggcga gcagaaacag cgcgcgttac
    16501 aggcgctgac ccgcgttggc atggtgcatt ttgcccatca gcgcgtttcc accctctccg
    16561 gcggacagca gcagcgtgtg gcgattgccc gcgcgctgat gcagcaggcg aaggtgattc
    16621 tggccgatga acccatcgcc tcgctggacc cggaatccgc ccgcatcgtg atggacaccc
    16681 tgcgcgacat caaccagaac gacggcatca ccgtggtcgt cacgctacat caggtggatt
    16741 acgccctgcg ctactgcgaa cgcatcgtcg ccctgcgcca ggggcacgtt ttctacgacg
    16801 gcagcagtca acagtttgat aacgaacgtt ttgaccatct ctaccgcagc attaatcgca
    16861 tcgaagagaa cgcgaaagct gcctgacatc cccatcattg aggaaaacga atgaacgcta
    16921 agataattgc ctcgctggcc ttcaccagca tgttcagcct cagcaccctg ttaagcccgg
    16981 cacacgccga agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc
    17041 aaaacctgaa accgcaatgg acgccattct tacaggatat ggagaagaag ctgggcgtga
    17101 aggtgaacgc cttctttgcc ccagactacg caggcattat ccagggaatg cgcttcaata
    17161 aagtggatat cgcctggtac ggcaacctgt cggcaatgga agcggtggat cgcgccaacg
    17221 gccaggtctt cgcccagacc gtcgcggcgg acggatcgcc gggttactgg agcgtgttga
    17281 tcgtcaacaa agatagtccg atcaacaacc tgaacgatct gctggcgaag cggaaagatc
    17341 tcaccttcgg caatggcgat cctaactcca cctctggctt cctcgtcccc ggctactacg
    17401 tcttcgccaa aaacaatatc tccgccagcg acttcaagcg caccgtcaac gccgggcatg
    17461 aaaccaacgc gctggccgtc gccaacaagc aggtggatgt tgccactaac aacaccgaaa
    17521 acctcgacaa gctgaaaacc tccgcgccgg agaagttgaa agaactgaaa gtgatctgga
    17581 agtcgccgct gatcccaggc gatccgatcg tctggcgtaa aaacctttcc gaaaccacca
    17641 aagacaagat ctacgacttc tttatgaact acggcaaaac gccggaagaa aaagcggtgc
    17701 tggaacgcct gggctgggcg ccgttccgcg cttccagcga cctgcaactg gtgccgattc
    17761 gccagctcgc actgtttaaa gagatgcagg gcgtaaaaag caataaagga ctgaatgagc
    17821 aggacaagct ggcgaaaacc accgagattc aggcgcagct ggatgacctg gaccgcctga
    17881 acaacgcgtt aagcgcgatg agttcggtga gtaaagcggt gcagtaatgt gctcgatcgg
    17941 tcccctctcc cttgagggag agggttaggg tgagggggaa tgcgcacgct gatgccctca
    18001 ccccggccct ctcccacagg gagagggaga aaacattaaa cggagccaaa catgcaaacc
    18061 atcaccatcg ccccacccaa gcgtagctgg ttctcgcttc tgagctgggc cgttgttctc
    18121 gccgtgctgg tcgtctcgtg gcagggcgcg gaaatggccc cgcttacgct gattaaagac
    18181 ggcggcaaca tggcaacctt cgctgccgac ttcttcccgc ccgatttcag ccagtggcag
    18241 gattacctca ccgaaatggc cgtcacgctg caaatcgccg tctggggcac cgcgctggca
    18301 gtggttctct ccatcccctt cggcctgatg agcgccgaaa acctggtgcc gtggtgggtt
    18361 taccagcccg ttcgccgcct gatggacgcc tgccgcgcca ttaacgaaat ggtcttcgcc
    18421 atgctgttcg tggtcgccgt cggtctcgga ccgttcgctg gcgtgctggc gctatttatc
    18481 cacaccaccg gcgtgctctc caagctgctt tccgaggcgg tggaagcgat tgaacccggc
    18541 ccggtggaag gcattcgcgc caccggtgcc aacaagctcg aagagatcct ctacggcgtg
    18601 ctgccacagg tgatgccgct gctgatctcc tactccctct atcgcttcga atccaacgtc
    18661 cgctcggcga ccgtcgtcgg catggtcggc gcaggcggga tcggcgtcac cctgtgggaa
    18721 gcgattcgcg gtttccagtt ccaacaaacc tgcgccctga tggtgcttat catcgtcacg
    18781 gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc actttatctg ataagcgagg
    18841 cattgatatc tatgcacttg tctacacatc cgac
//
LOCUS       DAFATA010000086        18786 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFATA010000086 DAFATA010000000
VERSION     DAFATA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.087"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..876
                     /gene="rlmKL"
                     /locus_tag="LGJ20_003537"
     CDS             <1..876
                     /gene="rlmKL"
                     /locus_tag="LGJ20_003537"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBK2387551.1"
                     /translation="QEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKA
                     RQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVN
                     LTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTTVDMSR
                     TYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAF
                     DVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDF
                     ARNRQIHNCWLITAA"
     gene            888..2795
                     /locus_tag="LGJ20_003538"
     CDS             888..2795
                     /locus_tag="LGJ20_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2387552.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAVPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            2925..4178
                     /gene="pqiA"
                     /locus_tag="LGJ20_003539"
     CDS             2925..4178
                     /gene="pqiA"
                     /locus_tag="LGJ20_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBK2387553.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            4183..5823
                     /gene="pqiB"
                     /locus_tag="LGJ20_003540"
     CDS             4183..5823
                     /gene="pqiB"
                     /locus_tag="LGJ20_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBK2387554.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            5820..6383
                     /gene="pqiC"
                     /locus_tag="LGJ20_003541"
     CDS             5820..6383
                     /gene="pqiC"
                     /locus_tag="LGJ20_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBK2387555.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            6639..6806
                     /gene="rmf"
                     /locus_tag="LGJ20_003542"
     CDS             6639..6806
                     /gene="rmf"
                     /locus_tag="LGJ20_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBK2387556.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6876..7394)
                     /gene="fabA"
                     /locus_tag="LGJ20_003543"
     CDS             complement(6876..7394)
                     /gene="fabA"
                     /locus_tag="LGJ20_003543"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBK2387557.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(7463..9223)
                     /locus_tag="LGJ20_003544"
     CDS             complement(7463..9223)
                     /locus_tag="LGJ20_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBK2387558.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            9409..9861
                     /gene="matP"
                     /locus_tag="LGJ20_003545"
     CDS             9409..9861
                     /gene="matP"
                     /locus_tag="LGJ20_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBK2387559.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            complement(9936..10988)
                     /gene="ompA"
                     /locus_tag="LGJ20_003546"
     CDS             complement(9936..10988)
                     /gene="ompA"
                     /locus_tag="LGJ20_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBK2387560.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(11345..11854)
                     /gene="sulA"
                     /locus_tag="LGJ20_003547"
     CDS             complement(11345..11854)
                     /gene="sulA"
                     /locus_tag="LGJ20_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBK2387561.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            12073..12702
                     /gene="sxy"
                     /locus_tag="LGJ20_003548"
     CDS             12073..12702
                     /gene="sxy"
                     /locus_tag="LGJ20_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBK2387562.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(12665..14827)
                     /gene="yccS"
                     /locus_tag="LGJ20_003549"
     CDS             complement(12665..14827)
                     /gene="yccS"
                     /locus_tag="LGJ20_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBK2387563.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(14837..15283)
                     /locus_tag="LGJ20_003550"
     CDS             complement(14837..15283)
                     /locus_tag="LGJ20_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK2387564.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            15406..17460
                     /gene="helD"
                     /locus_tag="LGJ20_003551"
     CDS             15406..17460
                     /gene="helD"
                     /locus_tag="LGJ20_003551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBK2387565.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(17492..17950)
                     /gene="mgsA"
                     /locus_tag="LGJ20_003552"
     CDS             complement(17492..17950)
                     /gene="mgsA"
                     /locus_tag="LGJ20_003552"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK2387566.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(18046..18708)
                     /locus_tag="LGJ20_003553"
     CDS             complement(18046..18708)
                     /locus_tag="LGJ20_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBK2387567.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         4812 a         4535 c         4983 g         4456 t
ORIGIN      
        1 caggaaggaa ttgaatgtta ccgcctgtat gacgccgatc tgccggaata taacgttgcc
       61 gttgaccgtt atgccgactg ggtggtggtg caggagtatg caccgccaaa aactattgat
      121 gctcacaaag cgcgtcagcg tctgttcgat attatcgctg caaccatttc ggtactgggg
      181 attgcgccaa acaaactggt gctgaaaacc cgtgaacgtc agaagggcaa aaatcagtac
      241 cagaaactgg gcgagaaggg cgagtttctg gaagttaccg aatataacgc tcacttgtgg
      301 gtgaatctga cggattacct cgataccggt ctgttcctcg atcaccgtat cgcccgtcgt
      361 atgctcggcc agatgagcaa aggcaaagat ttcctcaacc tgttctctta taccggcagc
      421 gccaccgtgc atgcgggatt aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt
      481 acttatctgg agtgggcaga acgcaacctg cgtctgaatg gcctaaccgg gcgtgcgcat
      541 cgcctgattc aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg
      601 atctttatcg atccgccaac cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt
      661 cagcgcgatc atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg
      721 atcatgttct cgaacaacaa acgtggcttc cgtatggatc tcgacgggct ggcaaaactg
      781 ggactgaaag cacaagaaat tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc
      841 cagatccaca actgctggct gattaccgca gcctgaaagg aatagtaatg tcattaatca
      901 gtatgcatgg tgcatggctg tcgttcagcg acgcgccgct tctcgataac gcagaactgc
      961 atatcgaaga taacgaacgt gtttgtctgg taggccgtaa cggcgcgggc aaatcgacgt
     1021 taatgaagat cctcaaccgt gaacaggggc tggatgacgg gcgcattatt tacgagcaag
     1081 atttgatcgt ggctcgtctg caacaggatc cgccgcgtaa cgttgagggc agcgtttatg
     1141 atttcgttgc cgaaggcatt gaagaacaag cggaatacct gaaacgctat cacgatattt
     1201 cgcgcctggt gatgaacgac ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg
     1261 aacagctgga tcaccacaac ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc
     1321 aactggggtt agatcctaat gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag
     1381 cggcattagg acgcgcgctg gtgagcaatc cgcgcgtttt gctgctcgac gaaccgacta
     1441 accacctgga tatcgaaacc atcgactggc tggaagggtt ccttaaaacc ttcaatggga
     1501 cgattatttt catctcccac gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg
     1561 atctcgatcg cggcaagctg gtgacctatc cggggaatta cgaccagtac ctgcaggaaa
     1621 aagaagaagc cctgcgcgtg gaagaactac aaaatgccga gttcgatcgc aaactggcgc
     1681 aggaagaggt gtggatccgc caggggatca aagcacgccg tacccgtaat gaaggccgcg
     1741 tacgcgccct gaaagcaatg cgtcgcgagc gtggtgaacg tcgcgaagtg atgggtaccg
     1801 caaagatgca ggtggaagag gccagccgct ccggtaagat cgttttcgaa atggaagacg
     1861 tttgctacca ggttgacggt aagcaactgg tgaaagattt ttcggcccag gttctacgtg
     1921 gcgacaaaat tgccctgatt ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga
     1981 tgctcggtca gcttcaagcg gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg
     2041 cttatttcga tcagcaccgc gcggaactgg atcccgataa aacggtgatg gataaccttg
     2101 ccgaaggtaa gcaagaggtg atggttaatg gcaagccacg ccacgtattg ggctatttgc
     2161 aggactttct gttccatccg aaacgggcga tgacgccggt acgtgcgctt tctggcggtg
     2221 agcggaaccg cttgctactg gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg
     2281 acgaaccgac caacgatctt gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca
     2341 gctatcaggg cacggtattg ctggttagcc acgatcgtca gtttgtcgat aacaccgtta
     2401 cagaatgctg gatcttcgaa ggcggcggta aaattggtcg ttatgttggc ggttatcatg
     2461 atgcccgtgg tcagcaagaa cagtatgtgg cgctcaaaca gcctgcggtg aaaaaaagcg
     2521 aagaagccgc cgtgccaaaa gcagaaactg taaaacgcag cagtagcaaa ctaagctata
     2581 aattgcagcg cgaactggag cagctaccgc aattgcttga agatctggag gcgaagctgg
     2641 aagctttaca gacgcaagtg gcggatgctt ccttcttcag tcagccgcat gagcagacac
     2701 aaaaagtgct cgccgatatg gctgctgcag agcaggagct ggagcaagcc tttgaacgct
     2761 gggagtatct tgaagcgtta aaaaatggtg gctgatcgca aaatagtcga tttaaacaga
     2821 gacagctgac aacggtaaaa ttgcctgatg aactgggttt atcaggcatg aaaaccaaca
     2881 ctttgttagt catctgtaaa atagcgcatc attaaggagt accaatgtgc gaacatcatc
     2941 atgccgcgaa gcacatcctg tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg
     3001 agcatggtca gaaagcggca tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg
     3061 cccctcggca gcgtcctacc gcctatgcgt tggctgcact gttcatgctg ttgctgtcca
     3121 acttgtttcc ttttgtgaat atgaacgttg caggagttac cagtgaaatt acattactgg
     3181 aaattcccgg cgtgcttttt tctgaggact acgccagcct cggcaccttt ttcctgttgt
     3241 ttgtgcaact ggttcccgcg ttttgtctga taaccattct gttactggtg aatcgcgcgg
     3301 aattaccggt ccgtttaaaa gagcaactgg cacgggtgct ttttcaactc aaaacctggg
     3361 gaatggcgga gattttcctc gctggtgtgc tggtcagttt cgttaaactg atggcttacg
     3421 gcagcattgg ggtaggcagc agctttctcc cctggtgttt attttgtgtc ctgcaactgc
     3481 gcgcttttca gtgcgttgat cgtcgctggt tatgggacga catcgccccg atgccagaac
     3541 tgcgccagcc gctaaaacca ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct
     3601 gttgtacggc aatccttcct gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt
     3661 acgttcggcg tagaaacagc ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc
     3721 tgtatcttcc ggctaatatt ttgcccatca tggtgacgga tttattaggc tcgaagatgc
     3781 cgtcgacgat tctcgctggg gtcattctgt tatggagcga aggctcttat cccgtcgctg
     3841 cggtgatctt tctggccagt attatggtgc caacgttaaa gatgatcgcc atcgcgtggc
     3901 tgtgttggga tgccaaaggg catggcaagc gcgacagtga aagaatgcat ttgatttatg
     3961 aagttgttga gtttgtaggc cgctggtcga tgattgacgt tttcgttatc gcggtgctct
     4021 cggcgctggt gcgtatggga ggtttaatga gtatttatcc ggcaatgggt gcattaatgt
     4081 ttgctttagt cgtcataatg acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt
     4141 gggatcgtca acctgaatca gagcatgagg agtcctgaca gtatggaatc taataatggg
     4201 gaagccaaaa tccagaaagt gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg
     4261 gcgctcattg gggcctgggt tcttttttat cattacagcc atcagggacc ggaagtgacc
     4321 ctgatcaccg cgaatgcgga aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc
     4381 gttgacgtcg gcgtggttga aagcgccaca ctggctgatg atttgacgca cgttgaaatc
     4441 aaagcgcggc tgaattccgg tatggaaaaa ttgctgcata aagacaccgt cttttgggtg
     4501 gtgaaaccgc agattggtcg cgaagggatt agcggcctgg gaacgctgct gtctggagtt
     4561 tatatcgaac tgcagccagg cgcgaaaggc agcaaaatgg ataaatacga tttgctggac
     4621 tcgccaccgt tggccccgcc tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa
     4681 gccgggcagc tctcgccagg agatccggtg ctgttccgtg gctatcgggt aggttcggtt
     4741 gaaaccagca ccttcgatac acaaaaacgc aatatcagct atcaactgtt catcaatgca
     4801 ccttatgacc gactggtgac caacaatgtt cgcttctgga aagatagtgg cattgcggtt
     4861 gatctgacgt cagcagggat gcgtgtggag atgggctcat tgacaacgct gctgagtggc
     4921 ggtgtcagct ttgatgtgcc ggaaggtctg gatttagggc agccagtggc acctaaaaca
     4981 gctttcgttt tgtatgatga tcagaagagc attcaggatt cgttgtacac cgatcacatt
     5041 gattatctga tgttctttaa agattcggta cgcggtctgc aaccgggagc tccggtagag
     5101 ttccggggta ttcgcctggg taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt
     5161 cagacattta acgatgatta ccgtattccg gtactgattc gtatcgagcc agagcggctg
     5221 aaaatgcagc ttggcgaaaa tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt
     5281 ggtttacgcg gatcgctgaa aaccggaaac ctggtcactg gtgcactgta tgttgatctc
     5341 gatttctatc caaatacgcc tgcaataacc ggtattcgtg aatttaatgg ttatcagatt
     5401 atcccgaccg ttagcggcgg cctggcgcaa atccagcaac gactgatgga agcgttggat
     5461 aagatcaaca aactgccatt gaatccgatg attgaacagg caaccagtac gctttctgaa
     5521 agtcagcgca caatgaaaaa cctgcaaacg acgctggata gcatgaacaa gatcctcgct
     5581 agccagtcga tgcagcagtt gccgacggat atgcagtcaa cgttgcgtga attgaatcgc
     5641 agcatgcagg gcttccagcc tggctccgca gcctacaaca agatggtggc ggatatgcag
     5701 cgccttgatc aggtgttgcg agaactgcaa ccggtgctga aaacgctcaa tgagaagagt
     5761 aacgcgctgg tatttgaagc gaaggacaaa aaagatccag agccgaagag ggcgaaacaa
     5821 tgaaaaagtg gctagtgacg attgcagcac tgtggctggt cggatgcagc tccggcgaaa
     5881 ttaataaaaa ctattaccag ttacctgtgg tgcagagcgg tacacaaagt accgccagcc
     5941 agggcaatcg tctgttatgg gtagagcagg tcactgttcc tgactatctg gcggggaatg
     6001 gtgtggttta tcaaaccagt gatgtgaagt atgtgattgc caacaacaac ttgtgggcca
     6061 gcccgttgga tcaacagttg cgcaacaccc tggttgccaa cctgagtacg caactgcccg
     6121 gctgggtggt tgcctcccag cctctgggaa gcgcccagga cacgctcaat gttaccgtaa
     6181 cggagtttaa cggtcgctat gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc
     6241 accagggaca actgatcaaa cgtccgttcc gtctggaagg agtgcaaact caggatggtt
     6301 acgatgagat ggttaaagtg ctggccggtg tctggagtca ggaagccgct tctattgcac
     6361 aagagataaa gcgtctacct taattataaa gatttgtaaa tataaccgtc tccggtatgt
     6421 tgcctgaggc ggtttttttg tttctaacgt gcggaaaaat ttgttcctct tcacattttt
     6481 tgtacaaccg acatgcccgt gtagctcaca aatatgacag tggcgtgaat tttgcgcatt
     6541 gacggcagtt atgattcgcg gtattgctta actgtgattg cacatttagt aatcactgtt
     6601 ttcttttcca ccagaaacca gtatgaggga aacgaggcat gaagagacaa aaacgagatc
     6661 gcctggaacg ggcacatcaa cgtggttatc aggccggcat cgccggacgc tcaaaagaaa
     6721 tgtgtcccta tcagacgctg aatcaaaggt cacaatggct gggaggctgg cgagaagcca
     6781 tggcggacag ggtagtaatg gcctgattct gtctctttaa aaagaaacct ccgcattgcg
     6841 gaggtttcgc cttttgatac tctgtctgat tataatcaga aggcagacgt atcctggaac
     6901 agaccgactt tcaggtcgct ggcggtatag ataagacgac catcaaccag cacttcgcca
     6961 tccgccaggc ccataatcag acgacggtta acaatgcgtt taaagtgaat acggtaggtc
     7021 acttttttcg ctgtcggcag tacctgacca gtgaatttca cttcgccaac gcccagcgcg
     7081 cggcctttac cttcgccgcc cagccagccg aggtagaacc ctaccagctg ccacattgcg
     7141 tccaggccca ggcatcccgg cataaccgga tcgccaataa agtggcatcc gaagaaccac
     7201 agatccggat tgatatccag ttctgcttca acataccctt tgtcgaagtt accacccgtt
     7261 tcggtcattt tgaccacacg gtccatcatc agcatgttcg gtgctggcaa ttgcgggcct
     7321 ttagcgccaa acagttcacc gcgaccagag gcaagaaggt cttcttttgt ataggattcg
     7381 cgtttatcta ccatgttctc tgtaagcctt attttattga agcacgcagg atagctaaca
     7441 cgtgtacgct gaacaagtcc gatcagttcg gaataaacca gttcagccaa cgtaatggcc
     7501 atggaaaacg gtgacgtcct tcctgttgcg atgcttgcgc gatacgttcc tggatggttt
     7561 gcatcagcgt cgtttggcct tcgccatccc acaccagatt taataataac ggcagtgcgt
     7621 cagtcacatc gtctactgcc cagatggtga atttgccttc ttctaccgct ttcaccagtt
     7681 cactgtgaag acttaaatgg cgaacgttag ctgtggggat aatgacacct tgtttcccgg
     7741 ttaactcacg ttgctggcaa atagcaaaga agccttcgat tttctcattt aaaccaccga
     7801 ccggctgggc gcgaccgaac tgatcgactg aacctgtgat agcgatactc tgattcaccg
     7861 gcacatcggc gagggcgctt atcagggcgc agagttcagc catcgaggca ctatctccat
     7921 caacttcact gtatgactgc tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa
     7981 gctgtagttc cgacatcagg aacgcttgca tgatcatcat ccctttcgca tggatattgc
     8041 cgccaagctc cgctttgcgt tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc
     8101 agctaatgcg agaaggttcg ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa
     8161 gggcgttaat ttgcccgatg cgttcgcctt cggtttcaat caggatttgc tcctgaagga
     8221 tctcatcctg catacgttca gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta
     8281 agtttagctg ctcgccggag aaggtgtcgc catcacacag ggaggcgacc tctttacact
     8341 ggcggaggat ccactgcggg ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg
     8401 cttcgcggat aagtaccggc caggcatccg ctcccggtgc aggtaagtga ttatgtctgg
     8461 cggtaaatgt cacccagcga caccactggg ttactgactc tgcatcgaca atctgcagag
     8521 tatcttcaaa ttcgctataa atagcctgct ctgaaagctc tggctccatc tcctggaaat
     8581 cagccaatga ttcgcgttcg cctaccagaa tgactttcag cttcaatggc atcgaaggca
     8641 cagagacggg gagagggcgc gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa
     8701 cgatattttt cagccgcatc cacagcagag gttgcgccag cagtgtacgc aaagagatga
     8761 tgagaatacc gccatttgcc tgatgcacca gaccaggctg cagggtaatg tcgccattaa
     8821 actggcgcag gcagccaaag agttgctccg cttctaccca gtcggcagcg acaacttgcg
     8881 ttaaagtcgc aaaattatca tctgcactca ctgcgtgacg taagcggatg gagtggccgg
     8941 aaacctcata gtgaccgccc accagttggc ctgcatcgct ttgtagcgta cgcgcggcat
     9001 tggcaataag attcagatac tcagactctt ccggggcctt cgccagcata aaagaggagg
     9061 atgctcgcgt atgcagcaac tgctccagtg cgaattgcag tcgcggttga gtatcactga
     9121 ataaaggatc gttttcgtca atcaaatgtg gctgagcaaa tatttcctga tagctatcgg
     9181 tatcaggaac caggtcacgc catgcaagtt tcgtaatggt caaagttgat gttttttagt
     9241 ctgttgtcaa agccgcgatt ataccgtaac cggcactaca gcacacgtag aaagcaccga
     9301 caatactcct ggcatgggcg ttaaagctca caggatggag attcttttct tcactggcct
     9361 aaaaagctga tattctgtaa agagttacac ggtaacattg agatcgctat gaaatatcaa
     9421 caacttgaaa atcttgaaag cggttggaaa tggaagtatc tggtgaagaa gcatcgcgaa
     9481 ggggagttaa tcacccgtta catagaagcc agtgccgccc aggaagccgt cgatgtgttg
     9541 ctctcgctgg aaaatgaacc cgtgctggta aatggctgga ttgacaagca tatgaatccg
     9601 gaactggtca atcgcatgaa gcaaaccatt cgggccagac ggaagcgcca ttttaatgcg
     9661 gaacaccagc atacgcgcaa aaaatcgatc gatctggaat ttattgtctg gcaacgtctg
     9721 gctggtcttg cgcagcgtcg cggtaaaacg ctttctgaaa cgattgttca gctgattgaa
     9781 gatgcggaaa acaaagagaa atacgcgaat aaaatgtctt ctttgaagca tgatctgcag
     9841 gcattgctgg gtaaggaata actgacgaag tcagttcaat ttactaaagg cgaaaaaaac
     9901 cccgcagctg cggggttttt ctaccagacg agaacttaag cctgcggctg agttacaacg
     9961 tctttgatgc ctttaacttc gatctctacg cgacgatccg gagccaggca gtcgatcagt
    10021 gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata
    10081 ccacgtgcgg agatcttgtc tgccgggata cctttggaga tcaggtaatc aacaacagac
    10141 tgagcacggc gctcggacag accctggttg taagcgtcag aaccgatgcg gtcagtgtaa
    10201 cccagaacaa ctacggaacc gtctttcgga tccaggttgc tcagctggct gtacagctga
    10261 tccagagcag cctgaccttc cggtttcagg gttgctttgt tgaagttgaa cagaacgtca
    10321 gacttcagag tgaagtgctt ggtctgtact tccggtgccg gagctggagc cggagcaact
    10381 accggagctg cttcgccctg accgaaacgg taggaaacac ccaggctcag cagaccgttg
    10441 tccggacgag taccgatggt gtttgcgtca ccgatgttgt tggtccactg gtattccaga
    10501 cgggtagcga tttcaggagt gatcgcgtac tcaacaccac ctgcgaaaac cggagaaacg
    10561 ccagtgtcgt ggtctttaaa ggatgcgcca ccaggtacgt tagccttggt gtctgcacgc
    10621 cataccatac cacccagacg agtgtatacg tccagatcgt cagtgattgg gtaacccagt
    10681 ttagcagtca gttgaacgcc ctgagctttg tatgcaccgt tgatgttgtc gcctttgtac
    10741 ggcatacgac ctaaccagtc gtaacccatt tcaaaaccaa catacgggtt aacctggtaa
    10801 ccaccaaaag cacctgcacc cagttggttt tcgtgggtcg gaccattgtt aggaataaaa
    10861 ccagtgtcat ggtactggga ccagcccagt ttagcaccag tgtaccaggt gttatctttc
    10921 ggagcggcct gcgctacggt agcgaaacca gccagtgcca ctgcaatcgc gatagctgtc
    10981 tttttcattt tttgcgcctc gttatcatcc aaaatacgcc atgaatatct ccaacgagat
    11041 aacacggtta aatccttcac cgggggatct gctcaatatt aactctaccg atatcttcgg
    11101 cttatgccga gcacccctgg cgatgtaaag tctacaacgt agttgaaaac ttacaagtgt
    11161 gaactccgtc aggcatatga aaaaaaagtc ttgtataagg tatgtttaat cttttttgtc
    11221 agcgacaatt tacagaagaa aatcgcggaa accgcttcag acaagcctcc gcaaggaaaa
    11281 ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag ttccaggatt aatcctaaat
    11341 ttacttaatg atacaaatta gagtgaattt ttagcccgga aagttgtctc gtggcgtgag
    11401 aggatgcgct taccggacgc ataataaacc ccatagcgtt accttcattt gccgcatcaa
    11461 caagttcagc atgctcttct tcagtcaaat catctgccaa ccaaccgatc accacactgt
    11521 aattgcccgt gcgtaaagcg cgaaccattg actccacagt gtggcaaggg gagagctggc
    11581 taatctgcat tactttcgtt aagggtagcc cagatgcctg aacccattcc cgactcagtt
    11641 tttgttgcgg tgttaaccag agttgccagc gcgattgctg accgagttgc tgtaacaatg
    11701 gcaacagtag aagttgcgtc atcatgggct gatcttcgcg atagacaact tcactgataa
    11761 gcccggctgt agtgttttcc gtagagacac gcgcaatttt acttgctgcg gatgagaacg
    11821 acgaagaacg atgtgcatag cctgaagtgt acataatcaa tccagcccct gtgagttact
    11881 gtatggatat acagtacatc cagtgacaac aaagatcaac cctattttcg gaaagagcct
    11941 cgcaaatttt gtcgttggtg acgggaaaac ataaattaat cttgcccctt aagaataagt
    12001 tgcctatttt cgtagttaac ggatccgtta atgtgaatca ttcttttatg ttatgatttt
    12061 aaaaggaatt ttatgaaaag cctctcctat aagcggatct ataaatcaca agaatacctg
    12121 gcaacgttgg gcacaattga ataccgatca ttgtttggca gttacagcct gaccgttgac
    12181 gacacggtgt ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg tgagcaaagt
    12241 gcacagtact gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg tggccgatcc
    12301 gttaccctca attactatcg ggttgatgaa agtctatggc gaaatcaact gaagctggtg
    12361 cgtctgtcga aatattctct cgatgcagcg ctgaaagaga aaagcacgcg caatacccgg
    12421 gaaagactga aagatttgcc caatatgtct tttcatctgg aagcgattct cggggaggtg
    12481 gggattaagg atgtacgggc gttacgtata cttggggcaa aaatgtgttg gttgcgactg
    12541 cggcagcaaa acagtctggt gacagaaaag attctgttta tgcttgaagg tgccattatc
    12601 ggcattcatg aagctgcgct cccggtggca cgccgccagg agcttgcaga atgggctgac
    12661 tctcttacgc cgaaacagga gtttcctgcg gaacttgagt aatctggcgt tgcagacgac
    12721 caatctcagg cagtaatgca atcaataatc caacttgttg tacgaccaga ggttctttgc
    12781 tgtctgcccg tggttcaagt tgctgcatcc gctgtttcag gctagctaat gcctcattga
    12841 cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc aacatagcaa actgcgtcat
    12901 caagaaacgc cagaatttca ggattagtta actgctcccg gtgagcaccg agggctgaga
    12961 tatagctggt aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg cgaatttgcg
    13021 gggtaacgtt cggctcgctg gacatatttg ataccaccga cgccagctca gcatcacggt
    13081 tgtgtgcatc gcggcgggca atacgatacg ccagacggtt atcacgcccc tgatggtatt
    13141 gctccagtat ggcatcgaga taccgacagt tggcctctgt ggcgcgttcg agcatgcgcg
    13201 gcagattgcg aaactgccag tcaggccaga tgtagctcac tgccgcccac gcaatggcac
    13261 aaccaatcag cgtatcgatt acgcgaggta acgctacttc aaaaccttca cccagtaagt
    13321 taaaacacag tagcaccaaa agtgtgatga acatcgttgc atgagcgtat tgcacgttac
    13381 ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac cagctgccct tccagtgatg
    13441 gcacaaacca cagcacagga atgccaatgg cgatacctac cagcgtacca ataatcctta
    13501 acttcaggcg gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa cttgtcagca
    13561 agatccaata cccgtgatgc attccggtta tttgaatgat ggcgtagccg aagcacaaca
    13621 ccagcgacat tcttaccgca tgacggaaga gggcggattc cggcgtgaag tgacggctaa
    13681 gacgcagcca gatatcactc aacccgtgcg ggctgtcatc agcgagctca ttttcgtcat
    13741 tattatgggg tagtgcctgg gcctgttctg attcaattgt tgccagttgg gcatcaatgg
    13801 cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa atcggcgggt gcgccgttat
    13861 cgcgcatccg ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa tgcggatcat
    13921 gttgataagg ctgacgcagc aaaatacagc gtgacagttg ctggcacgcc tggccctgca
    13981 tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt tcacgcaatg
    14041 tttgatactg aatatgagaa gagctggcac gctcgtgaat atcctgcgcg acaaagtaat
    14101 aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg gtcagcagcg
    14161 agagtttcgt ctgattcaat gtcgccatca gttgtccgtt ggcgagagcc aaatcgtaca
    14221 gcggtgcctg gctttcatct tcaatatcag gatcaaacat gcgcgacttg agctcaagat
    14281 aacgcgccag ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg accgggaaca
    14341 gcagatgacc aataagtgtc aggacgttgt accagacggc accggccagc agatacatcg
    14401 gctgctgata ccagtgctca tacagtgatg ttcccaacat agtgtaaatg gcgatcagca
    14461 atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa atgaagccgc
    14521 tggtagagag cgttaagcca atcgcaaata gccagggcca gggaaacagc aattctactg
    14581 aggccgaggc gataaaaaag cagaacagcg taatgatgag gttacgcaaa cgtcccgcca
    14641 gtcggtcatc gagatcggtc agcgccgctg ccaccatccc cagcgttagc ggaatcgtca
    14701 gttttacatc acccagccac cacggaaacg ctgtggttcc acaaagcgca ataaaaatac
    14761 gcgcgtaata cagccaggcg ctgttccagg tatagcgttt gagcaaagga cttagcataa
    14821 aggccatatt agtggattat tcaaaacgac gacgtgcatt ggcttcacgc gcagcttgcg
    14881 ctgtttctac cgataccacg cgacgaccaa ccggccatag tgcaatagcg gcaattttaa
    14941 agttcgcaat gccgacagga atgccaatga ttgaaataca ttgtgcgatg cccgttgcaa
    15001 tgtgcatcag gcataaccac cagccaaaga aaatcagcca gaaaatattc aataccgtac
    15061 cgccagtatt cagcagcaca tttttgccag ccgggttcag ttcatcgaca tgaatagctt
    15121 cattgccata aggcaccaga gacagtttag tgatctccca gcaggatcgt gtcagcggta
    15181 aggtaaaaat cagcacaata ctgaccagag tcgccaacag ccagcccaga gtggtggcaa
    15241 atccgccaag cacaaagttc agaatgttca aaacggtacg cataaaacct cgctttactg
    15301 tggttttcag taatgggcgg caattgtaac gtttttttgg gctggagcac gttttctctg
    15361 acggttacac tgataagaaa taatttcgtg tggatctaca gagtcatgga actgaaagcg
    15421 acaacgcttg gaaaacgtct ggcacagcac ccttacgatc gggcggtgat cctcaatgcc
    15481 gggattaaag tctccggcga tcgccacgaa taccttattc ctttcaatca attactggcg
    15541 attcactgta agcgcggtct ggtatggggc gagctggaat ttgtactgcc ggacgaaaaa
    15601 gtggtgcgtc tgcacggcac cgaatggggc gagacgcagc gtttttacca tcatcttgat
    15661 gctcactggc ggcggtggag tggcgagatg agcgaaattg cgtctggtgt tttacgccag
    15721 caactggatt tgattgccac gcgcactgga gaaaataaat ggctgacgcg tgagcaaacc
    15781 tctggcgttc agcaacaaat ccgccaggct atgtcggcgt tgccgttgcc ggttaaccga
    15841 ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat gtcaggcctg gttgaaagat
    15901 attgaaagcg ctcggttgca gcataaccag gcgtataccg aagccatgct taccgagtat
    15961 gcggattttt tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc ccgggcagtc
    16021 gttaatggcg agcattctct gttagtgctg gcaggtgcag gaagcggaaa aacgtcggtg
    16081 ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga gcaaatttta
    16141 ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg agcgtattcg cgaacggcta
    16201 cataccgaag acattaccgc acgcacgttt catgcgctgg cgctgcatat tattcagcag
    16261 ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg ataccgctgc ccgtcatgaa
    16321 ctctttattg ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc gaagggctgg
    16381 cggcaatggc tgacggaaga aatgcagtgg tcagtgccag aaggtaactt ctgggatgat
    16441 gaaaaattac agcgtcgtct tgcctcacgc ctcgatcgtt gggtaagtct gatgcggatg
    16501 cacggtggtg cacaggcaga aatgattgcc agtgcacccg aagagattcg cgatctgttc
    16561 agtaaacgta tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc gctgaaggca
    16621 gaaaacgctg tcgatttttc gggccttatt catcaggcga ttgtgattct ggagaaaggt
    16681 cgctttatca gcccgtggaa gcatattctg gttgatgaat ttcaggatat ctcgccgcag
    16741 cgggcagcgt tgttagcggc attacgcaag caaaacagtc agacgacgtt gttcgccgtt
    16801 ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc aaatgtcgct caccaccgct
    16861 ttccatgaaa actttggtga aggcgatcgc tgtgatttag acacgactta ccgttttaac
    16921 agtcgtatcg gtgaggtggc aaaccggttt attcagcaga acccaggcca gttgaaaaag
    16981 ccgctaaaca gcttaaccaa tggagacgaa aaagccgtca cgttattgga tgagagtcaa
    17041 cttgacgctt tgctggataa gctctctggt tatgccaaac cggaagagcg cattcttatc
    17101 ctggcgcgtt accatcacat gaggcctgcc agcctggaaa aagcggcaac acgctggccg
    17161 aagttgcaaa tcgactttat gaccattcat gccagcaaag ggcaacaggc ggattacgtc
    17221 atcatcgttg gcttgcagga gggaagtgat ggttttccgg ctgcggcgcg ggagtcgatt
    17281 atggaagagg cgctactgcc accggttgag gatttcccgg acgctgaaga acggcggtta
    17341 atgtacgtgg cgctgacccg ggcacgccat cgggtatggg cactgtttaa caaagagaat
    17401 ccctctccct ttgtggaaat actgaaaaat ctggatgtgc cggtggcgag aaaaccgtaa
    17461 gaaacaggtg gcgtttgcca cctgtgcaat attacttcag acggtccgcg agataacgct
    17521 gataatcggg gatcagaata tcgaccgcgt cgttgaaatg cggcgactgg attatgaagt
    17581 ctgccgttgc cacgttggtg gcgaccggaa tgttccatac cgtcgccaga cgcagcaagg
    17641 ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc ccagaagaaa atcaatacat
    17701 caattttccc ttctgagatc aatgcgccaa cctgctggtc accccccatt gggccactca
    17761 acatcgcgtt gacgttcatg ccggtcgcgc gggaaattaa gttaccggta gtgcctgttg
    17821 catacagtac gtgttgttcc agtaacggtt gatgccgttc cacccagctc atcagcattt
    17881 gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa gtgcgagtcg
    17941 tcagttccat aatgtacatc cgtagttaac tttcctacag attactgtaa gcacttatcg
    18001 ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa aaatctcagg aagatggttg
    18061 tttttccgcc cactgcagga aagtatttcg cgtttgtggg tcagccagtt taaaccaata
    18121 cttcagccgt tgttctgtga gcacctgaga ctgcggtgga atagcatcca gctcggaaac
    18181 acccgagagc agcgtactgt catctgccat catcgtggca aattgcggta gtgaagcgcg
    18241 tttcgcggat ttgttatagc gttcaacctc gacctcataa tcaatcgttt ttgccgttga
    18301 ggtaatggcg agaatatcca gctttaccgg aatcggcgtc gcatcgccat ccaacaattc
    18361 aaggcgcggc gcggcatcaa aatggttagc ttcccgctca ttctccaggc gaggcaggcg
    18421 aaaattgacc tggttgatga gctgggtatt aaaactgacc accagcggtg gggagatata
    18481 cagccgttct tcactattgg aaagatgaat cgtcttctca acgcgaaaca ctaactgatg
    18541 cggtccatta tccagttcaa tgctgtccgc accgcgcagc agagagctgg aaaccttctt
    18601 gccgtcgagt accagaagat cgacatcggt tgaaagccgc agcgtggtgg caaaaacgga
    18661 taccggcaaa cataatgcaa tcaaggtggt cacgatgccg gttttcatag gtagctcctg
    18721 tcaaaagacc gcccgaataa taatgtgtca aaatttttct ttaatcatac ttactaacat
    18781 atagac
//
LOCUS       DAFATA010000087        18664 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFATA010000087 DAFATA010000000
VERSION     DAFATA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.088"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..146)
                     /locus_tag="LGJ20_003554"
     CDS             complement(<1..146)
                     /locus_tag="LGJ20_003554"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBK2387568.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMI"
     gene            complement(236..2170)
                     /gene="ftsH"
                     /locus_tag="LGJ20_003555"
     CDS             complement(236..2170)
                     /gene="ftsH"
                     /locus_tag="LGJ20_003555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK2387569.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(2270..2899)
                     /gene="rlmE"
                     /locus_tag="LGJ20_003556"
     CDS             complement(2270..2899)
                     /gene="rlmE"
                     /locus_tag="LGJ20_003556"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBK2387570.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            3025..3318
                     /gene="yhbY"
                     /locus_tag="LGJ20_003557"
     CDS             3025..3318
                     /gene="yhbY"
                     /locus_tag="LGJ20_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK2387571.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(3474..3950)
                     /gene="greA"
                     /locus_tag="LGJ20_003558"
     CDS             complement(3474..3950)
                     /gene="greA"
                     /locus_tag="LGJ20_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK2387572.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            4198..5631
                     /gene="dacB"
                     /locus_tag="LGJ20_003559"
     CDS             4198..5631
                     /gene="dacB"
                     /locus_tag="LGJ20_003559"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBK2387573.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(5671..6843)
                     /gene="cgtA"
                     /locus_tag="LGJ20_003560"
     CDS             complement(5671..6843)
                     /gene="cgtA"
                     /locus_tag="LGJ20_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBK2387574.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(6859..7824)
                     /locus_tag="LGJ20_003561"
     CDS             complement(6859..7824)
                     /locus_tag="LGJ20_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2387575.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(7951..8208)
                     /gene="rpmA"
                     /locus_tag="LGJ20_003562"
     CDS             complement(7951..8208)
                     /gene="rpmA"
                     /locus_tag="LGJ20_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK2387576.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(8229..8540)
                     /gene="rplU"
                     /locus_tag="LGJ20_003563"
     CDS             complement(8229..8540)
                     /gene="rplU"
                     /locus_tag="LGJ20_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK2387577.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            8799..9770
                     /gene="ispB"
                     /locus_tag="LGJ20_003564"
     CDS             8799..9770
                     /gene="ispB"
                     /locus_tag="LGJ20_003564"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBK2387578.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            9997..10275
                     /gene="sfsB"
                     /locus_tag="LGJ20_003565"
     CDS             9997..10275
                     /gene="sfsB"
                     /locus_tag="LGJ20_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBK2387579.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(10323..11582)
                     /gene="murA"
                     /locus_tag="LGJ20_003566"
     CDS             complement(10323..11582)
                     /gene="murA"
                     /locus_tag="LGJ20_003566"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK2387580.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(11637..11891)
                     /gene="ibaG"
                     /locus_tag="LGJ20_003567"
     CDS             complement(11637..11891)
                     /gene="ibaG"
                     /locus_tag="LGJ20_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBK2387581.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(12051..12344)
                     /gene="mlaB"
                     /locus_tag="LGJ20_003568"
     CDS             complement(12051..12344)
                     /gene="mlaB"
                     /locus_tag="LGJ20_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBK2387582.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(12344..12979)
                     /gene="mlaC"
                     /locus_tag="LGJ20_003569"
     CDS             complement(12344..12979)
                     /gene="mlaC"
                     /locus_tag="LGJ20_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBK2387583.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(12998..13549)
                     /gene="mlaD"
                     /locus_tag="LGJ20_003570"
     CDS             complement(12998..13549)
                     /gene="mlaD"
                     /locus_tag="LGJ20_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBK2387584.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(13554..14336)
                     /gene="mlaE"
                     /locus_tag="LGJ20_003571"
     CDS             complement(13554..14336)
                     /gene="mlaE"
                     /locus_tag="LGJ20_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBK2387585.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(14344..15153)
                     /gene="mlaF"
                     /locus_tag="LGJ20_003572"
     CDS             complement(14344..15153)
                     /gene="mlaF"
                     /locus_tag="LGJ20_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBK2387586.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLGIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            15363..16340
                     /locus_tag="LGJ20_003573"
     CDS             15363..16340
                     /locus_tag="LGJ20_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBK2387587.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            16354..17340
                     /gene="kdsD"
                     /locus_tag="LGJ20_003574"
     CDS             16354..17340
                     /gene="kdsD"
                     /locus_tag="LGJ20_003574"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBK2387588.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            17361..17927
                     /gene="kdsC"
                     /locus_tag="LGJ20_003575"
     CDS             17361..17927
                     /gene="kdsC"
                     /locus_tag="LGJ20_003575"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBK2387589.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            17924..18499
                     /gene="lptC"
                     /locus_tag="LGJ20_003576"
     CDS             17924..18499
                     /gene="lptC"
                     /locus_tag="LGJ20_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBK2387590.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            18468..>18664
                     /locus_tag="LGJ20_003577"
     CDS             18468..>18664
                     /locus_tag="LGJ20_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="LptA; periplasmic binding protein part of a
                     putative ABC superfamily of uptake transporters; interacts
                     with LptB protein, the ATP binding component of the uptake
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBK2387591.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTI"
BASE COUNT         4582 a         5079 c         4527 g         4476 t
ORIGIN      
        1 ttgatcatca gattcgcatg tttcaccgca tctatcagcg agttatgcgt gccaccatcc
       61 gaaaaggaat caggcgtgac gttgaggatc cccattacgt gaggatggct aaggtccagt
      121 gaagtaccct gggcaaagag tttcatgatg ttatccctgg tatgaattga taagaaaaaa
      181 ccccggagca cgccccgggg ttttcggtac aaatacagtc atctgatgcg ggaacttact
      241 tgtcgcctaa ctgctctgac atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg
      301 gacgaggagc ctttggacta ccattgtcgc cagaattgtt agaagcgcct ggttcttccc
      361 agcccgctgg cggacgtaca tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga
      421 tagtctcata tttcatgaga gcatctttca tcgcatgcag aatatccata ttgtcggtca
      481 gaagctgacg cgcacgatta tagttacgct caatcagtgc tttcacttcc tggtcgatga
      541 tacgtgcagt ttcatcggac atatgtttcg ctttcgctac gctacggccg aggaatactt
      601 caccttcttc ttccgcgtac agcagtggac ccaatttctc agagaagccc cactgggtca
      661 ccatgttacg tgccagattg gtcgcaactt taatatcgtt ggacgcaccg gtagaaacat
      721 gttccggccc atagatgatc tcttctgcca gacgaccacc gtacagcgta gaaatctggc
      781 tttccagttt ctgacggctg gcgctgattg cgtcgccctc aggcaagaag aaagtcacac
      841 ccagcgcacg accgcgtggg ataatcgtca ctttgtgcac cggatcgtgt tccggcacca
      901 ggcgaccgat aatcgcatga cccgcttcgt ggtaagccgt tgattctttc tgcgcttccg
      961 tcatcaccat ggagcgacgt tccgcaccca tcatgatttt gtctttcgct ttctcgaact
     1021 caaccatcga cacaacgcgt ttgttgccac gagcagcgaa cagtgccgct tcgttcacca
     1081 ggttcgccag gtcagcaccg gagaaaccag gagtaccacg ggcaatgatt gccgcgtcga
     1141 tatcgggggc caatggtacg cgacgcatgt gaactttcag gatctgctca cgaccgcgaa
     1201 catctggcaa gccgaccaca acctgacggt cgaaacggcc aggacgcagc agggccgggt
     1261 cgagaacgtc cggacggtta gtcgcggcga taacgatgat accttcgtta ccttcgaagc
     1321 catccatctc aaccagcatc tggttcagag tctgttcacg ttcatcgtga ccaccgccca
     1381 gaccagcgcc acgctggcgg cctacggcgt cgatttcatc gataaagatg atgcacggtg
     1441 ccgctttctt cgcctgttcg aacatgtcac gaacacggga tgcacccaca ccgacgaaca
     1501 tttctacgaa gtcagaaccg gagatagtaa agaacggaac tttcgcttcg cctgcaatcg
     1561 ctttcgccag caacgtttta ccggtacccg gaggaccgac catcaggacg cctttcggga
     1621 tcttaccgcc gagtttctgg aagcggctcg gctcgcgcag atactctacc agttcagcaa
     1681 cttcttcttt tgcttcgtcg cagcccgcaa cgtcagcaaa ggtcgttttg atctgatctt
     1741 ccgtcagcat gcgcgctttg ctcttaccaa acgacatggc acctttgcca ccgccgccct
     1801 gcatttgacg catgaagaag atccagacac caatcagcaa cagcatcggg aaccaggaga
     1861 tgaagataga agccagcagg cttggttctt caggcggttc accgacaacc ttgacgttct
     1921 tggtcaacag gttatccagt aatttcggat cctgaaccgg aatgtaagtg gtataacggt
     1981 tactatcttt cttggtaacg ttgatttcac gtccgttgat acgcgcttca cgaacctggt
     2041 cgttattgac ctcttgtagg aaggtagagt aatccacctt acggccatta gactcgctgg
     2101 gcccaaagct ctggaatact gacatcagca caacggcaat gaccagccag agtattaggt
     2161 ttttcgccat gtcactcaag ggattaacct cttattacaa ctgtgttaaa aacagcgtca
     2221 ggatactcta tatccagcat ctttcaaact ttcgtctgaa atctcccggt tagggtttac
     2281 gcccggtcgc tacaatatac acttcccgcg aacgtgcacg agaagagtcc ggcttacgaa
     2341 ctttgacctt cgtaaacagg gagcgaattt cccttagata ctcatcgaaa ccttcgccct
     2401 ggaacacctt cactacaaaa ctgccacctg gcgctaatac atcacgacac atttctagcg
     2461 ccagttccac cagatacatg gcacggggga tatccaccgc cggtgttccg ctcatgtttg
     2521 gtgccatatc ggacatgaca acctggactt tgctgtcgcc aacgcgctcc agcagtgctt
     2581 tcatcaccag ttcatcacga aaatcgccct gaagaaagtc cacaccaacg ataggatcca
     2641 taggtaaaag atcgcaagcg atgatgcggc ctttgccgcc aatttgggtg accacatatt
     2701 gtgaccaacc acccggagca gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt
     2761 tgtcactttg ctgtatttca tcaagtttaa accaggcacg ggaacgtaac ccctttttct
     2821 gtgcctgttg aacatattta tcgctaaagt gttcctgaag ccagcggctg gagctggcag
     2881 aacgcttctt acctgtcatt taactttccc atggggataa ctcatcgtaa ccaattgcgt
     2941 aaatttttac gcgcctattt ggtgatatat gggagatggc ggtagaatga cccgttttca
     3001 atcccaacgt aagcaaaaaa tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa
     3061 ggtctggcac atccgctcaa gccagttgtt ctgcttggca gtaatggttt gaccgaaggg
     3121 gtgctggccg agattgaaca agcgttagag caccatgaac tcatcaaggt gaaaatcgcc
     3181 accgaagatc gcgaaactaa aaccttgatc gtggaagcta tcgtgcgcga aaccggcgcc
     3241 tgtaatgtac aggtcatcgg taaaacgctg gtgctttatc gcccaactaa agaacgtaaa
     3301 atctcgctgc cacgctaaga ttatcctaaa gttacacaca tttgctgtgt aaaacgaggg
     3361 gttttccgca ggcaggagag caaaatgcca cgctctgttc gttgataaaa ggccgcatag
     3421 cggccttttt cctttcttta caatacatca acatcttgag tattgggtaa ttcttacagg
     3481 tattccacct taattacttc aaattctact tcgccgcccg gcgttttgat gaccacaaca
     3541 tcatcttctt ctttgccgat caggccacga gcaataggcg agtttacaga aatcaggttt
     3601 tgtttaaagt cagcttcgtc atcgccaacg atgcgataag tctgttcttc gtcagaatcc
     3661 agattcagca ccgttacggt agcaccaaaa ataacgcgcc cattgttggg cattttggtg
     3721 acatcaatca cctgcgcgtt cgacagcttg gcttcgatgt ctttaatacg gccttcgcag
     3781 aaaccctgct gttcacgagc tgcgtggtat tcggcgtttt ctttcaggtc gccatgctca
     3841 cgcgcttccg cgatagcagc aatgatttca ggacggcgca cagatttcag aaaatccagc
     3901 tcttcgcgta atttttcagc gccgcgtaag gtcatcggaa tagcttgcat ttgttatacc
     3961 tcttgaatat tcctgatggg gcaagtcttc acccaccccg gctgttcggc ctgcccggca
     4021 taactcctca ccgggaccag aagcaaaaaa ataccgaccc gggtacaagt cccaggtcag
     4081 ctacaattca cattttgata gtcattttac cctgaagttc ccgaagggtc atcgtttact
     4141 ttatagggcg ttgcgccgta gtatgacggc tcgattccag gttgttagcg cgagattatg
     4201 cgattttcca gatttatcat cggattgacc agctgtatag cgttcagtgt tcaggccgcg
     4261 aatgttgatg agtacgttac tcaactcccc gctggtgcca accttgccct gatggtgcaa
     4321 aaagtcggcg cgtcggcccc cgctattgat taccacagtc agcagatggc gctgcccgcc
     4381 agtacgcaga aagtgattac tgcgctggcg gcgttgattc aactcggccc cgattttcgt
     4441 ttcaccacga cgcttgaaac caaaggcaat gtggaaaacg gcgtacttaa gggtgactta
     4501 gtggcgcgat ttggtgccga tccgacgtta aaacgtcagg atattcgcaa tatggtcgcg
     4561 actttgaaaa aatctggcgt caaccaaatc gatggcaatg tgttgataga tacctccatt
     4621 ttcgccagcc acgataaagc ccccggctgg ccatggaatg acatgacaca atgctttagc
     4681 gctccgcctg ccgctgccat agttgaccgc aactgtttct ccgtctcgct ctacagtgcc
     4741 ccaaaacctg gtgatatggc ttttatacgc gtggcatctt attaccccgt tacgatgttc
     4801 agccaggtac gcaccctccc ccgaggttct gccgaagcgc aatactgcga actggatgtc
     4861 gtaccaggcg acctgaaccg ctttacgctg acgggatgcc tgccacaacg ttctgagccg
     4921 ctcccgttgg cttttgccgt gcaggatgga gccagctatg ccggggcaat tctgaaagat
     4981 gagttaaaac aggcaggtat cacctggagc ggaacactgc tgcgccagac tcaggttaac
     5041 gaacctggaa cggtagttgc cagtaaacag tcggccccgt tgcacgatct gcttaagatt
     5101 atgctgaaaa agtcggacaa catgatcgcc gatacggttt tccgcatgat aggccatgcg
     5161 cgcttcaatg tgcctggaac atggcgggcc gggtcggacg ccgtgcgtca gatcctgcgc
     5221 cagcaagccg gtgtcgatat tggaaacacc attattgccg atggttcagg gctttcgcgg
     5281 cataacctga ttgcccccgc caccatgatg caggtgctgc aatacattgc ccaacacgac
     5341 aatgaactta actttatctc catgctgccg cttgcgggct atgacggctc tttgcagtac
     5401 cgtgcaggtc tgcatcaggc gggcgtggat ggaaaagtct cagcgaaaac cggttcgttg
     5461 cagggggtat ataacctggc gggattcatt accacagcga gcgggcaacg aatggcgttt
     5521 gtgcaatatc tttctggcta tgcagtagaa cctgcggatc agcgtaatcg ccgtattccg
     5581 ttagtgcgtt ttgaaagccg tttgtataaa gatatttatc agaacaatta gtcaaaaaga
     5641 aaccccggca catggccggg gttttgctga ttaacgcttg taaatgaact caacgccttc
     5701 ttcgtcgtct tcgtcccagt catcatccca gtcctcatca tcctcttcag caatctcttc
     5761 aagctgctgg cgatgataat catcccacat gaattcgact ttctctggct gtttcgcttc
     5821 ttcagcctgc acgaccgggt tttcaatgat aaaggtcatc acatcccagc agagatcttt
     5881 cacgcccagt ccactcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
     5941 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
     6001 cactaaccaa cgtggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
     6061 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
     6121 caggacgcgg caacgttcca ggtgcttcag gaagcgaatg cccagacctg cgccttccgc
     6181 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
     6241 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
     6301 cgccgatacc gcacgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
     6361 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcgcccggcg taccgttagt
     6421 tttctgccgt ggtgtacggt taactgacga tttgaaacgg gtattgccca gaccgtgcca
     6481 gccgccctta gcaaccagca gacgctgacc gtgtttggtc atatcgccca tggtttcacc
     6541 agtaccctgg tcgattacac gcgtacctac cggcacttta attgtgacgt ctttaccgcg
     6601 cttaccggta cagtcgcggc ttgcgccatt ctgaccgcgc tctgcacgga aagatttttc
     6661 aaaacgataa tcgataagcg tgttcaggtt ctcgtcggct tccatccata cgtcaccacc
     6721 atcaccgccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
     6781 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
     6841 cattttattc tccgtaaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
     6901 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
     6961 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
     7021 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
     7081 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaaggt
     7141 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
     7201 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
     7261 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
     7321 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
     7381 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
     7441 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
     7501 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
     7561 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
     7621 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
     7681 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
     7741 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
     7801 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
     7861 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
     7921 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
     7981 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
     8041 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
     8101 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
     8161 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
     8221 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatggccc
     8281 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
     8341 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
     8401 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
     8461 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
     8521 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
     8581 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
     8641 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
     8701 ccattttttc agtacaatca ctctatattc ctaaccataa accctaagtt gcctttgttc
     8761 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
     8821 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
     8881 acgtccagct gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
     8941 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
     9001 ccattgctgc cctgatcgag tttatccaca cggcgactct gctgcacgac gacgttgtgg
     9061 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc tgcatttggc aatgccgcca
     9121 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
     9181 cgctcaaagt gctggaagtc atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc
     9241 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
     9301 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
     9361 gtacgccgga agaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
     9421 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
     9481 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
     9541 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
     9601 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
     9661 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
     9721 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
     9781 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
     9841 tataacacct gaaaaaaagg gcgatgaatc tttatatatt ctgaatattc acactcttta
     9901 caggaacttt ttagagcaat agccatcagg agtatagtga tgcccgacag aagaagtgtt
     9961 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    10021 catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct
    10081 cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa
    10141 ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc
    10201 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    10261 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac
    10321 gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg
    10381 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    10441 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    10501 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc atgcgcgccc
    10561 atacggctaa gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    10621 ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    10681 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    10741 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    10801 gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc
    10861 gccaccagga aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct
    10921 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    10981 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    11041 atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    11101 actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa
    11161 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    11221 ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    11281 accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    11341 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    11401 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagttttgg gacgttctgg
    11461 atctctaccg gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta
    11521 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    11581 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    11641 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca
    11701 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    11761 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    11821 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    11881 ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa
    11941 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    12001 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    12061 agaacatcag caggcaaatt atacaatttc gccagggtat acactttgtc gtttaccccc
    12121 tgaagcgtca cattgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    12181 agtccccccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc
    12241 gcttcctcac gcatttccca aagcggtagc aaaacgtcct gatccagctc tccggataac
    12301 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    12361 gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg
    12421 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    12481 tcatgtcgta agcctgccag ttgcccgtct gggagttttt acgccactgg aagtccagac
    12541 gcaccggcgg gcggccattc ggatcaataa tggtaacgcg aatcggcaca atggttttat
    12601 cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    12661 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag
    12721 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    12781 atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    12841 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttataag
    12901 gattggtctg gtctgccgcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca
    12961 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    13021 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    13081 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag
    13141 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    13201 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    13261 tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    13321 ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg
    13381 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt
    13441 cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg
    13501 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    13561 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    13621 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    13681 aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc
    13741 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg
    13801 cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc
    13861 gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc
    13921 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    13981 tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc
    14041 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    14101 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    14161 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt
    14221 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    14281 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    14341 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    14401 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    14461 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    14521 aatacctaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    14581 gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa
    14641 aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg
    14701 ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac
    14761 ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggaagtt gggtatgttc
    14821 gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    14881 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    14941 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    15001 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg
    15061 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    15121 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    15181 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg
    15241 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    15301 ccgttacggc tttctgaaaa tcttcagcgg accggcgagt atacctgaag aaaggacgtt
    15361 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    15421 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    15481 tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg
    15541 cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca
    15601 atatattgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattctgatg
    15661 ttctacgccg tgaattaccc ttaatgttgt tggtcagcgt ggtggccggt tccgtactct
    15721 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    15781 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    15841 gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg
    15901 gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc
    15961 tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa
    16021 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    16081 ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg
    16141 cgctgataac gccaggagag attgatccac tggcgtacag tcgtgactac agcgtgatgt
    16201 tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg
    16261 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    16321 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    16381 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    16441 cttgatcaat acatcaatca gaatttcacg cttgcttgtg aaaagatgtt ctggtgtaaa
    16501 gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggcggca
    16561 acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    16621 gatttaggca tggttacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc
    16681 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    16741 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    16801 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt
    16861 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    16921 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    16981 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcattg
    17041 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    17101 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    17161 cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg
    17221 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    17281 ggcgaccatt tactcggtgt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    17341 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    17401 ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg
    17461 atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt
    17521 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    17581 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    17641 acttgtatca ggggcagtca aataaactga tcgcctttag cgatctgctg gaaaaattgg
    17701 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    17761 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    17821 attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac
    17881 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    17941 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcatta atatggccga
    18001 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    18061 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    18121 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    18181 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    18241 ccggatgctc tatttatatg gacacgttga agtcaacgca ctcgtgccgg actctcaact
    18301 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    18361 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    18421 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    18481 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    18541 catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc
    18601 ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca
    18661 tcaa
//
LOCUS       DAFATA010000088        18408 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFATA010000088 DAFATA010000000
VERSION     DAFATA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.089"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1640)
                     /gene="yihQ"
                     /locus_tag="LGJ20_003578"
     CDS             complement(<1..1640)
                     /gene="yihQ"
                     /locus_tag="LGJ20_003578"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBK2387592.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNW"
     gene            complement(1839..2741)
                     /locus_tag="LGJ20_003579"
     CDS             complement(1839..2741)
                     /locus_tag="LGJ20_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBK2387593.1"
                     /translation="MQITNMHCSGQTVSLTAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(2879..4120)
                     /gene="yihS"
                     /locus_tag="LGJ20_003580"
     CDS             complement(2879..4120)
                     /gene="yihS"
                     /locus_tag="LGJ20_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBK2387594.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(4137..5015)
                     /gene="yihT"
                     /locus_tag="LGJ20_003581"
     CDS             complement(4137..5015)
                     /gene="yihT"
                     /locus_tag="LGJ20_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBK2387595.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(5039..5935)
                     /gene="yihU"
                     /locus_tag="LGJ20_003582"
     CDS             complement(5039..5935)
                     /gene="yihU"
                     /locus_tag="LGJ20_003582"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBK2387596.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6103..6999
                     /gene="yihV"
                     /locus_tag="LGJ20_003583"
     CDS             6103..6999
                     /gene="yihV"
                     /locus_tag="LGJ20_003583"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBK2387597.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7033..7818
                     /locus_tag="LGJ20_003584"
     CDS             7033..7818
                     /locus_tag="LGJ20_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2387598.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIVIILPQEDA"
     gene            7917..8516
                     /gene="yihX"
                     /locus_tag="LGJ20_003585"
     CDS             7917..8516
                     /gene="yihX"
                     /locus_tag="LGJ20_003585"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBK2387599.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8510..9382
                     /locus_tag="LGJ20_003586"
     CDS             8510..9382
                     /locus_tag="LGJ20_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBK2387600.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9379..9816
                     /gene="dtd"
                     /locus_tag="LGJ20_003587"
     CDS             9379..9816
                     /gene="dtd"
                     /locus_tag="LGJ20_003587"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK2387601.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9861..10802
                     /gene="fabY"
                     /locus_tag="LGJ20_003588"
                     /gene_synonym="yiiD"
     CDS             9861..10802
                     /gene="fabY"
                     /locus_tag="LGJ20_003588"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBK2387602.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(10866..11774)
                     /locus_tag="LGJ20_003589"
     CDS             complement(10866..11774)
                     /locus_tag="LGJ20_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2387603.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            12003..12314
                     /locus_tag="LGJ20_003590"
     CDS             12003..12314
                     /locus_tag="LGJ20_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2387604.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            12315..12605
                     /locus_tag="LGJ20_003591"
     CDS             12315..12605
                     /locus_tag="LGJ20_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2387605.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            13210..13428
                     /locus_tag="LGJ20_003592"
     CDS             13210..13428
                     /locus_tag="LGJ20_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBK2387606.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKRGRQRGETVGVYDQCKDHKE"
     gene            13648..13890
                     /locus_tag="LGJ20_003593"
     CDS             13648..13890
                     /locus_tag="LGJ20_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387607.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(14220..15149)
                     /gene="fdhE"
                     /locus_tag="LGJ20_003594"
     CDS             complement(14220..15149)
                     /gene="fdhE"
                     /locus_tag="LGJ20_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBK2387608.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(15146..15781)
                     /gene="fdoI"
                     /locus_tag="LGJ20_003595"
     CDS             complement(15146..15781)
                     /gene="fdoI"
                     /locus_tag="LGJ20_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBK2387609.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(15778..16680)
                     /gene="fdoH"
                     /locus_tag="LGJ20_003596"
     CDS             complement(15778..16680)
                     /gene="fdoH"
                     /locus_tag="LGJ20_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBK2387610.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(16693..>18408)
                     /locus_tag="LGJ20_003597"
     CDS             complement(16693..>18408)
                     /locus_tag="LGJ20_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBK2387611.1"
                     /translation="RGHSNIQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKP
                     LLEGQVNYWGNYPKFFVSMMKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEG
                     KVNGYICQGFNPVASFPNKNKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSS
                     KIQTEVFRLPSTCFAEENGSIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKM
                     YAEQGGANPDQVLNMTWNYAIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQL
                     SSFAQLRDDGTTSCGCWIFAGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRIL
                     YNRASADPQGNPWDPKRQLLKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLF
                     ALDKMAEGPFPEHYEPFETPLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTT
                     YRLTEHFHYWTKHALLNAILQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAV
                     VTKRIRTLKANGKDIDTIGIPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLV
                     NVEKV"
BASE COUNT         4397 a         4663 c         4812 g         4536 t
ORIGIN      
        1 tgccagttgt cgccaggacg gttaccttcg tgggtgcgca tcatcggcgt gaaggcgctg
       61 aaatcgcacc agcgcagcag cagctctttg ctgcgcttca tctcaaacag ggtggtgtaa
      121 ccgccaatgt cgctgtggtg caggccatgt ccggtcattg ccagcgacag cgccgccggg
      181 acaaccgacg ccaggccatc gtcgagactc cagtcgacgt tctggtcgcc cgcccacatc
      241 atggtggagt atttctggct accggtagaa ccggcgcgca taaagaaaag gatctcgccg
      301 agcttgcccg tttcttcaag ggcttcgtag ttacacttcg cccacagcgc aggccaggcg
      361 ttatgcataa tttcggcact gacgccgtta tgcaggtacg tgtcggtggg cagatactcg
      421 ccgaagtcag ccatccagcc gccgcagccg agttcaatca tgttcttttt gatcacttcc
      481 ttgaaccagg cgtaggcttc tggattagtg agatcgacaa cgccgccgta aaactcgcca
      541 aactccacca gatagtcacc gccagaggca tcttttgcca gatagccgtg ttgtgccgct
      601 tcttcgcaga gatctttatc gctggcaaca tacgggttga tataggccag gaactgcacg
      661 ccctcctgat tccactgctt aatgcgtgaa tccagttgcg ggtagttttc gctgttccac
      721 ttccagttcc acatcacgcg tttgccaaaa gaggtcatac gaataccgga ccagtcctgc
      781 gcccagatgc cgttgacctt cacgcccgcg ttacgcatgg tgtccagttt cttctggcac
      841 acttccgtcc cgccctgaat gccgagcgtt actccgtcat aaatccagtc gggcagttct
      901 ggctggcgtc ccagcagggc ggttaatttt tccagcaggg aaatgtatgt gtcagcacat
      961 tcaaaacgca gcgttgcttt gtcttcccac agcgccagtt catggtattc cggggcacta
     1021 aagtcgaagt tcatatagca actgttatca acatggcaat aatacttctg cgtgctgaca
     1081 aacgtaggct gtgggaagaa agtccagtaa tagtcgccgc ccgcattttc tttgcagtcg
     1141 gcctgccagg tgacataggt ttgtttgttg cgaccaacgc cttgttcact ggtccatagc
     1201 gggaacggtt tgccacgcag atcgaagtag gaaaactgtt cgccgcagcc gtagatatgg
     1261 tcctctggtt gagcggcaag gcgcagccag atacggttgt ggttaaggtt gtcgttttgt
     1321 agttccagca ataaacggcc ctgatcgtcg gcagagatat tcagcgtggc gctaatgtca
     1381 gaaccacggc tgaaatgaat taaccaacca tccggtgact ggctgacgat ggcgtcggta
     1441 agcgcaattt tctcctgtag tttatcttta atgctgaaat taccgcggaa catatcgata
     1501 tccgctatac ctgagccaat ccataaacaa ggattatctt tgctatgggt taaaataaga
     1561 cgttgttgaa aatgtagggt aaaactgtcg ttattctgat gaaattgaaa atctaataac
     1621 tgtggacgtg gcgtatccat aactaatctc catagagttg agaacgagat tgttgccttc
     1681 ctgttattac aggcttatgt aaaatgattc tcataaaact gatatataaa aactttacag
     1741 aagctgagct gtttttaact taataaattg aagatacata atgttgcctt tagcgagtga
     1801 aatgttcttc aatgattttg actgctaatc aatacaagtt attttctctg tccgtaaatg
     1861 ttaaaaaaca ggcggtgcgg ttttcctggt tccagtaata ataagtcgat acctgaatta
     1921 aaggcgttag gcgggcaact cataggttca actgccagtc cctgacgctg taatttttca
     1981 ccgctataaa tttgtaccca gggttgatcg caacaaaggc taaccgaaag cgcctgttgt
     2041 ggatgggtaa tggttatttc ccacagatca ttagcggttt taaaggtgtg gtcgattttt
     2101 gtcgcggcta tttttttcgc ctgcgtgaaa tttaaatcca gctcatcaac gtggtgaagc
     2161 gttgtcggat ttacgtgttc atcaacggca taaacctggt ttgccggaag ctggaacaga
     2221 tattcatcaa cagatgttaa gttgcaggtt aaataaggat gaatacccac accatagggt
     2281 gcggtcacag taccaatatt ctgacttgcg atctcaacgg aaagacccgt gcgtgcattg
     2341 agcgaataaa caacctgcga agccagcata aacggatagc cgtaactggg cggcagaaaa
     2401 gctgtcaggg taacgctggt ggcggttaat tcactaattt gccagtctcg ccaggcaagc
     2461 agcccgtgaa tagcggcttt tgaactatgt tcattaatgg gtaactgata ttcctgcccc
     2521 tgatagcggt aacagccgtt ggcgatccgg ttcggccacg gaataagcac tttacccaga
     2581 tgcgccagcg gcatctcttc gggtttgtgc gggatgacta aatgacaacc ctgaaaggtt
     2641 aattcagcca gaccagcacc gacggtcaca atggtcgcgt gataatcccc cgcggtaagt
     2701 gaaacggtct gtccactgca atgcatattg gtgatttgca tacaaggcac cttaattaac
     2761 gacatacagc ctcccgtgag gctgatgtca aacccaaaaa ctgcaccgga cggtcccctc
     2821 gcccctttgg ggagagggtt agggtgaggg gaaaacccgc accctatttt cagatcagtt
     2881 atttcgcatt aatatccagc agacccgctg caaccgctgg agccatgccg ggcgctaacg
     2941 ggatacgcgg gatcaccagg caatgcagca ggtgataaat atcctgtttg ccgtcccaga
     3001 ctttggtggt gaccttattg tccgcatcca gctcctgcca ccaggaacca ttttcatagt
     3061 ccatcaggta cttaatgcag tactcccacc atgtttgata ccaggtttca tactggcgat
     3121 cgccagtgac ggtgtagagc gcgtaggccg tacccattgc ttcgacgata ggccaacgta
     3181 cacgttcgcg gaccaccggt tttccttccc agtcaacggt ataaacaatc ccgtccgcgc
     3241 catcgggtgc ccaggcatcg cgcacggtgg cgttaaacag acctttggca tcttctagca
     3301 gccatgctgg tggttgttcg caacgggctt ccagggcagc atggatgtgc agcattaaac
     3361 ggccccattc gatccagtgg cctggtgtac ctccaaacgc gcggaagcga tgcgccgggt
     3421 tatctttgtt gtaatccggc agcggattcc actgggtatc gaagtgttcg ttaacgcgat
     3481 aatgattatt tctggcgacg tcgtggataa tcacggaagc cacgcgaatc gcgcgatcca
     3541 gccatttttt gtcgtgagtg acgtcataaa caatcaagaa agcttccacc gcgtgcatat
     3601 tggcattgcc gccgcggtac tcttcggttt tgctgaaggc ttcgtcccag gattccaggc
     3661 acatctgctc ttcttcgctc cagaaatatt tctcgataat ttcaatggtg taatcgagca
     3721 gcttgcgcgc ttccgggtga cccgttgtga cggcgctggc ggcacccagc agagcaaaga
     3781 agtgttgata gccctgtttg gaggcatcca ccacgccctc gtcattcacg caggcatacc
     3841 agccgccata ttttttatca cgcagtgcgc cgttcatggc tttgataccg tgatcaacca
     3901 acgagtaagc gccaggtcga cccatcgccg cagcaacgga ataaacgtgc aacatacgag
     3961 cggtgatcca cagatgggtg cccatctctt ctttgatttg ccctttattg cctaaccagc
     4021 caaaaccagt cggcactacg gaatttttac caaaatcaaa gatgcggtcg gtttcctgtt
     4081 ccagccaacg gttgtggctt agggtgttaa accatttcat tccttttatc ctcatcttag
     4141 cggcgtttag ccatcatttc gtcgacgata tcgccaagtt gttgtaattt cggtgcgcaa
     4201 acgtcacgca gcatcagctc gttgtctggt aaaccgacga ccgatgccca gactgcacgg
     4261 cctgccagga atcccgatgc gcccgccgtc attgccacgc gtacggcacg cgggaacagt
     4321 ttttcgtcga caccggaaga gaggatcacc catggcatat tgatatggtc attcagacgt
     4381 tgtgaagcac agagaagctc ttgttgcgga cctttgccat aaagcggcat ttcaactttg
     4441 tagaggtcag cgccactgtc gcccagctct ttggcggcat cgatgatcgc ttgttcgcga
     4501 tcgaatttat cgccacgacg cggtgggcgg acgactggct caatgatgct taccagaccg
     4561 tgtgagtggc acagttcgtt gaactctttc accatatcca gacgttgctg cgcatcttca
     4621 tcactacgcc acagcaccag cagttttaag gctttgccac cgtcttgttt gatctgtagc
     4681 ggattgattt tgcggtcaat aaccacgcta tcgaccggaa taccgttgcc aggaatgaac
     4741 tcatcggcgg cgacaatcat ggcgcaactt ttggcaatcg cgttttgctc aaccacctgg
     4801 cggtagcaga attgttgatc taccagaatc gccgaggcat aaggcgagag ggcctttgca
     4861 gcgttaactt tgaaatcagt taaaacgcta tcggctaccg gagcgggtgc cccagccgcg
     4921 gcaaacatca tgcgcatggc ttcgcgctga tcgaccgcca gcatggcaaa accgcccgat
     4981 gcgcgcgtaa tgtcgttgat ggtgtactta ttcattcatt tattccttta tccagtcgtt
     5041 acatttttac tttggcagtc atcccggcac tgacacggac ctgttccaga atggcggacc
     5101 agtcctggcg accgcgaccc gctgcgcgcg cctggctata aacctcccgt gaggcggccc
     5161 ccagcggcat tggcacatgc agctggttgg cgacatcaag ggcgatgcca agatccttat
     5221 gggcaagatc gatcatgaag gcgggagaaa gatctccgct gaggactttg tttggccagg
     5281 aagtggtgaa gtgaccttta ccggcggcgg taccgctcat cactttgacg gcaacatcga
     5341 agggaagatt cagggcttcg cacaaaacgg cggcttctgc cgaaagcgca ttgagcgcga
     5401 tgctcatgta gttgttgatg agcttaacgc ggatccccat gcccggaccg ccagagttga
     5461 tcaactcact gcccatcgcc atcaggatcg gtgtggcacg ttcaacttgt tcagcggtgc
     5521 cgccagccag cagtaacaga gtaccggtaa tggcatttgc agaagtacgg cctaccggaa
     5581 catccatcat gctgaagcct ttggcttgca tatcggcaat caatttatcg gtttgcagcg
     5641 gatggatggt ggacatatca atgaccagcg catcggtaga taagccttcg caaacgccgt
     5701 tttcaccgaa caacacgttg cgcaccagat cgccattcgg caacatggta atgataaatt
     5761 cggcatcttt agcggcctgc gccgggttgg cggcgggagt cgcacctttg tctaccagat
     5821 gccgcacagc ttcggcgttc acatcaaaga cgcgaagttg atgcccttgc tgcaataaat
     5881 tgctcgccat tggcgaaccc atttgtccta aaccgataaa cgcgattgct gccataaccc
     5941 tctcctgaat acagtgatgt cactttttgt catttatgac atgctttgct tgtctgtttt
     6001 tgatcgtatt tgtaatttat cgtcaaaaaa ttgacagccg tcacttttta aacaattggt
     6061 gaaattaaaa tgaacgcatc ccaaaatgtt taaggaatga ccatgattcg tgttgcttgt
     6121 gtaggtataa ccgtgatgga tcgcatctat tacgtggaag ggttaccgac ggagagcggt
     6181 aaatacgtgg cgagaaatta tacggaagtt ggtggcgggc cagcggcgac ggcagcggtt
     6241 gcggcggcaa ggctgggggc gcaggtcgat tttattggtc gcgtaggtga tgacgacacg
     6301 ggcaacagcc tgctggcaga gctggaatcc tggggcgtta acacccgtta caccaaacgg
     6361 tataaccagg cgaaatcttc gcaatccgcc atcatggtgg ataccaaagg cgagcggata
     6421 atcattaact accccagccc ggatctgctg cctgacgcag agtggttgga ggaaattgat
     6481 ttctctcagt gggatgttgt gctggcagat gtacgctggc acgacggcgc taaaaaagcc
     6541 ttcaccctgg cccgtcaggc gggtgtgatg accgttctgg acggggacat tacgccgcag
     6601 gatatcagtg agctggtggc attaagcgat cacgcggcct tttcagaacc gggtctggcg
     6661 cgcttaacgg gcgtgaaaga gatggccagt gcgctaaaac aggcacaaac gctcacaaat
     6721 ggtcatgtct atgtgaccca gggtagcgca ggctgcgact ggctggaaaa tggtgggcgt
     6781 cagcatcaac cggccttcaa agttgatgtg gtagatacca caggtgcggg tgatgttttt
     6841 cacggcgctt tggcggtggc gctggcaaca agtggggatt tagcggagtc agtccgcttc
     6901 gccagcggtg tagcggcgtt aaaatgcaca cgtcccggtg gacgagccgg gatccctgac
     6961 tgtgatcaaa cccgatcttt tttgtcactt tttgtataaa atgccagggt gatggttttt
     7021 cgaggaattt tcatgagcct taccgaacta accggtaacc cgcggcacga tcaactcctc
     7081 atgctgatcg ccgagcgtgg gtatatgaat attgatgagc tggcaaatct gctggatgtc
     7141 tccacgcaga cggtccgccg ggatattcgt aaattaagcg agcaaggcct gattacgcgc
     7201 catcacggtg gcgcgggtcg ggcttccagc gtcgttaata cggcgttcga gcagcgtgag
     7261 gtttcgcaaa ccgaggaaaa aaaagcgatt gccgaagcgg tggcagacta tattcctgat
     7321 ggttcaacaa tatttatcac cattggtacg actgttgagc atgttgcccg ggcgttactt
     7381 aaccataatc atttgcggat aatcaccaac agcctgcgtg tggcgcatat tctttaccac
     7441 aacccgcgct ttgaagtgat ggtgcccggc ggtacgttgc gctctcataa tagcgggatc
     7501 attggccctt cagcggcgtc ctttgtggct gattttcgtg ctgattatct ggtaacaagc
     7561 gttggggcga ttgagagcga tggcgcgttg atggagtttg atgtaaacga agctaacgtg
     7621 gtgaaaacga tgatggcgca cgcgagaaat attctgctgg tcgccgatca cactaagtat
     7681 catgcttcgg cggcggttga aattggtaac gtggcacagg tcactgcgct ctttaccgac
     7741 gagctgccgc ccgctgcgct aaaatcacgc ttacaagaca gccaaattgt aatcattctt
     7801 ccccaggaag acgcgtagat ttttgtgacc gttaacctgg cttcatacct tgccacatag
     7861 ccaaacccat cctttcccgc tacagttaat ttcttgtggc gcgaaaggag gcaaaaatgc
     7921 tctatatctt tgatttaggt aatgtgattg tcgatatcga ctttaaccgt gtgctgggag
     7981 cctggagcga tttaacgcgt attccgctgg catcgcttaa gaagagtttt catatggggg
     8041 aggcgtttca tcagcatgag cgtggggaaa ttagcgacga agcgttcgca gaggcgctgt
     8101 gtcatgagat ggctctaccg ctaagctacg agcagttctc tcacggctgg caggcggtgt
     8161 ttgttgcgct gcgcccggaa gtgatcgcca tcatgcataa actgcgtgag caggggcatc
     8221 gcgtggtggt gctttccaat accaaccgcc tgcataccac cttctggccg gaagaatacc
     8281 cggaaattcg tgatgctgct gaccatatct atctgtcgca agatctgggg atgcgcaaac
     8341 ctgaagcacg aatttaccag catgttttgc aggcggaagg tttttcaccc agcgatacgg
     8401 tctttttcga cgataacgcc gataatatag aaggagccaa tcagctgggc attaccagta
     8461 ttctggtgaa agataaaacc accatcccgg actatttcgc gaaggtgtta tgctaaaaac
     8521 cattcaggac aaagccaggc accgtacccg tccactatgg gcctggctaa aactactctg
     8581 gcaacgcatt gatgaggaca acatgacaac cctggcaggt aaccttgcct atgtgtcgtt
     8641 gctctcatta gtgccgctgg ttgccgttgt ttttgcgctt ttcgccgctt ttcccatgtt
     8701 ttccgacgtc agcattcagt tacgtcactt tatttttgcc aactttctgc ctgctactgg
     8761 cgatgttatt cagcggtata tcgaacaatt tgttgccaat tccaacaaga tgaccgccgt
     8821 tggggcgtgc gggctgatcg tcaccgcgtt attgctgatg tactccatcg atagcgcgtt
     8881 gaataccatc tggcgcagta aacgagcgcg accaaaaatt tactcgttcg ccgtgtactg
     8941 gatgatttta acgctggggc cgctgctggc aggggccagt ctggcgatca gttcctattt
     9001 gctctccctg cgctgggcga gcgatctcaa tactgtcatc gacaacgtgc tgcgtatttt
     9061 tccgctgctg ttgtcgtgga tctccttctg gttgctgtac agcattgttc ctaccatccg
     9121 cgtacctaac cgcgacgcga ttgtcggcgc gtttgtcgcc gcgctcctgt tcgaagcagg
     9181 aaagaaaggt ttcgcgcttt atatcaccat gttcccgtca tatcagctca tttacggtgt
     9241 gctggcggtg atccccattc tctttgtttg ggtctactgg acatggtgta tcgtcttgct
     9301 tggcgcggaa attactgtca ctctcgggga ataccgcaaa ctaaaacaag cagctgaaca
     9361 agaagaagac gacgaaccat gattgcatta attcaacgcg taacccgtgc cagcgtcacc
     9421 gtggagggag aagtgacggg cgaaattggc gcgggacttt tggtgttatt gggtgtcgaa
     9481 aaggatgacg acgaacagaa agcaaaccgt ctgtgcgagc gtgtgctcgg ctaccgcatc
     9541 tttagcgatg ccgaaggcaa gatgaatctc aacgtgcaac aggcgggcgg cagtgtactg
     9601 gtggtttccc agtttaccct cgccgcagat accgaacgag ggatgcgccc aagtttctcc
     9661 aaaggcgcat caccggatcg cgcagaggcg ttatatgact atttcgtcga acgctgccgc
     9721 cagcaagaga tgaacacgca aacaggacgc ttcgctgccg atatgcaggt atcgctggtc
     9781 aatgatggcc ccgtgacatt ctggttgcag gtatgagcca gcttccaggg ttgtcacggg
     9841 aaacaagaga gagtatcgct atgtatcacc ttcgggttcc acaaacagaa gaagaattag
     9901 agcgttacta tcagtttcgc tgggaaatgt tgcgtaagcc cctgcatcaa ccaaaaggtt
     9961 cggaacgcga cgcgtgggat gcgatggcgc atcaccagat ggtcgtcgac gagcagggta
    10021 atctggtggc ggtaggccga ctgtatatta atgccgacaa tgaagcgtcc attcgcttta
    10081 tggccgttca cccggacgtg caggacaaag ggttaggaac gctgatggcg atgaccctgg
    10141 agtcggtggc gcgtcaggaa ggcgttaagc gcgtgacctg tagcgcccgt gaagacgcgg
    10201 tggagttttt cgccaagctg gggtttgtta atcagggaga aatcaccacg ccaaccacca
    10261 cgccgattcg ccattttttg atgattaagc ccgtcgccac tctggatgat attttgcatc
    10321 gcggcgactg gtgcgcgcag ctgcaacagg cgtggtacga acatatcccg cttagtgaaa
    10381 aaatgggcgt gcgtattcaa caatataccg ggcaaaaatt tatcaccacc atgccggaaa
    10441 ccggcaatca gaatccgcac catacgctgt ttgccgggag tttattctcg ctggcgacgc
    10501 tcaccggttg ggggcttatc tggctgatgc tgcgcgaacg ccatctcggc ggaacgatta
    10561 ttctggcgga tgcgcatatc cgctacagca agccgattag cggtaaacct catgcggtag
    10621 ccgaccttgg tgccttaagc ggcgatctcg accgtctggc gcgcggacga aaagcacggg
    10681 tgcagatgca agttgaaatc tttggcgacg agacgccggg tgcagtgttt gaaggcacgt
    10741 atatcgttct gcccgcgaag ccatttggcc cgtatgaaga gggcgggaac gaagaagagt
    10801 aggggcgttt tacttgactg aaaaggtttt caatctgaaa accttttcag ggcccaggtc
    10861 cgaagttacc ttcccgctct ccaggcaaca tcccgtagtg ccgatcgcgc ggcctcgctg
    10921 ataccaccta actgaaaaaa tccgtggata acgccaaggt agcgttggca ggtgcattgc
    10981 actccttgcc ctgtcatgcg gtgatacaac gcttcacctt catcgcaaag tgggtcgtat
    11041 tcagcggtaa tgatatgtac aggtggcaag ccattgaaat catcgcgcca aagagggctg
    11101 gcttcaggat gcctgcgttc aatgcctggc agatacatct caaagccgct cagtaaagtg
    11161 tcgcgtgtga tgatgtaatc gtgaccattg cgtatgtagc tttcgaaatg cgcagtcgca
    11221 tcaagcatag gataaattaa gatgagttgt gccggttgcc actcgccagc acgctttaat
    11281 cgcaaagcag ttaccagcgc cagatgtccg cccgcactgt caccggcaag agttatacgt
    11341 tgtttatcaa cgccgagtcg ttcggcgtgt tgccagacta tttctgcacc tcgttgcgca
    11401 tcatcatggg cggcggggaa agtatgttcc ggtgccagcc gatactggac ggcaataacc
    11461 cgacattgcc cgtaataggc taactggcgc agctggttgt cgtgagttgc gaacccgccg
    11521 ctaacaaaac agccaccgta gtaataaatt atcgtcggga gtaatgtcgg ggcattcagc
    11581 ggcgacacta ttcgtaaggt catcccttca agttcgatag tctgaatatc tacccgcgtt
    11641 tctgtctcac ccgcaaggac agcgctggcg atatagccct ccctgcgctg cgcaatactt
    11701 tgttgacgcg atgaggggcg acccgcagcg ataaactttt cgactaactc tgcaattcct
    11761 ttttccagtg ccatagtaga acttattttg ctgtatgaat gtacagtctt atacacccgt
    11821 ttttgctgga tggatacagg gaaattcacg ggcgtgaaaa atctggaaag cgcctcgcaa
    11881 atctaaagac aaacccggca gagacgtggt tttgctggag cactatcagt aagctagtgg
    11941 tttcaagggt acgtcaatga cgtacaaaaa ggattgttac tctaagccaa tggcgtataa
    12001 ttatgctctt catcgaaacg gaaattttta ctgaagatgt tcaaaaactg ctgaccgacg
    12061 atgaattcag tcgttttcag tttttcctgg ccttgaatcc tgactatggt gaggtaatac
    12121 ctgaaactgg tgggttaaga aaggttcgat gggtttcagg aggaaagggg aaacgtgctg
    12181 gtgtcagggt tatttatttt catcaggtta aacattatga gatcagatta ctgcttattt
    12241 atcgtaaggg tattaaagat gatctgtctc cccaggaaaa agcaatgctc cggttgttaa
    12301 atacgaggtg gtaaatggat aaggcacttt ttgagcggtt gactcacagc atggcccaga
    12361 tgaatgaaat tattgaagga acgcgacagc cttcgcggac ttttgaagtt gatgctatga
    12421 aaatcaaaga aatacgccgc gcttcagggt tgtcacaatc taaatttgcc gatcttattt
    12481 ctgtaagcgt agacacgttg cgtaactggg agcaggggcg acgttcaccg accgggcctg
    12541 caaaggcttt gctgcgagcg attgccaatg atccgcaaca tgtgctgcaa gcacttaacc
    12601 gttagaggca tggttatgcc atacagaaag cactagcatt cagaactcct gtgaaagcgg
    12661 catagtggtc gcctgcccag gagttttgct atgacccgta atcgtgctga acgtcgccac
    12721 cctatgatgc gattaaaaaa actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga
    12781 tgataccggg gggttccgga aggaattcct tgtttcccga actgttacca acaaaattat
    12841 aacgatgagc aagtaaagac cggttggtgg cagtactgac cggtctgtga tggatgatta
    12901 atttcacctt aaatccgggt gctgttagga tgctgacatt tttcgggtac accattcaga
    12961 ttatgcaaat aaagatccaa tcaaaaaata aacatccctg aatataatat attcatagca
    13021 tccataactc atcctgggcg ataactaaac catttttgag taataaatga aacaactgca
    13081 acactcttat tatttctgga aaacacctcg caaaaacaaa acaacatgca aaaatccgct
    13141 ttttttaaat gattttccgg cgtaattttc gcacatacat taatgagtaa tatatgtaag
    13201 gtggtggtaa tggcgatgaa tacggttttt cttcatttat cagaagaggc aattaaacgg
    13261 ctgaacaagc ttcggggctg gcgtaaggtt tcacgatctg caattttacg cgaagcggtt
    13321 gagcaatatc tggagcggca gcaatttccg gtgcgtaaag caaagagagg caggcaaagg
    13381 ggtgagacag taggtgttta tgatcagtgt aaggatcata aggaatgaca aaaatttttt
    13441 tattcttgaa tataaaaaac cagatgccct tattctggta ttaatacaag gttgttttac
    13501 ttgaacttat aataactgca actgttacat catatctgga aaacgcctcg caaaaataaa
    13561 aaatgattcg taaatgagct ttctttaaag attcgacaag ggtacttttc tctccataga
    13621 ttcaaaaatg atattggcga gatatttatg aactctctgg taggtataga tatgggcaga
    13681 attttactcg atttatcgaa tgaggtgatt aagcaacttg atgatctcga ggtgcagcgt
    13741 aatcttcctc gtgcggacct attaagggaa gcggtagatc aatacctgat aaatcaatcg
    13801 caaacagcaa gaaccagtgt tcctggcatc tggcaagggt gtgaggaaga tggtgtcgaa
    13861 tatcagcgta agctgcgcga ggaatggtaa tgggaaaaaa tgatgtcaat cagattgctg
    13921 acaacgtgcg cgttgttcat gccggatgcg gtgtgaacgc cttatccggc ctacaaagtc
    13981 atgcaaattc aatatattga agtttattgg tagggctgat aagcgcagcg catcaggcaa
    14041 tcatacgctt gtcatcagtg acatacccat aaccttatcg atgtgttgga gggaagaacg
    14101 aggcaaacag acaattgcgg catgatcttg aatgggtaac gcggaatacg agagatggtg
    14161 tcgtcacacc ctatgagctg taatccgggc gaccagcgcc acccggaaaa cgtcagagat
    14221 tactcccctt cacccggaaa caggaacggg ttgatggaac tgcgggcata gccttcttgc
    14281 tccattcgcg cgtccagtac cagagaagcg aggtcatctg ccacggcttc aactttcggt
    14341 tctttttcct gataaagaat cttcaggtaa gtgccacagt catcgcagct ttcggcttta
    14401 atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctctg ctcgcagttg
    14461 ctgcatttta cgcgcactac gtgccattcg gtttcacaca ggttgcagtg caggtaacgc
    14521 agaccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagaacc acaaaccggg
    14581 caatattgac gttgctcgcc gtattcagcg cgggctttgc cggggatcag attggccatc
    14641 tgcgcccagt agagcgacag tgcagcccag ataaacggcg ctttatcgct gctgacggat
    14701 gagaaatcag aggcaaacag tgcgctggcc atatcttcca gctcctgagt tgatgccttc
    14761 tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag ctcagcaatc
    14821 agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat atccagcggg
    14881 ggcttgccct gtgcgctggc ttctttaatg cgcgcggtca gatccatctc cagcggatgg
    14941 tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag caaagcgcag gtaatcaccc
    15001 agcggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta taaattcttg
    15061 agccgaggga acaataacgg cggaatcata tccgccgtac gtttctcgct cgaacccagc
    15121 tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc ttgcggacct
    15181 cacggtacca gcgcgggtga tgtttcttcg cccaggcgct ggtaacccat ccttccacca
    15241 tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg ataaccacaa
    15301 ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc gggattgaga
    15361 aagcaggcgc aaaataagga cgccagataa tcacgccgct caccagcagc agaaccagga
    15421 aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg gtgtcaccta
    15481 cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca tcccgattga
    15541 ttaggttgtg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac ataaccacgc
    15601 cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc catgatttgc atcaaccagt
    15661 tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag cagaaggcgg
    15721 tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca cgtcgtttca
    15781 tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg cacggttcgg
    15841 accgacaccc acgtagtgga agatactggc cgcaaaggta gccgcaaagc caacagccgc
    15901 gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg ggttctccgg
    15961 caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacatga cgtgtgtacc
    16021 accgacgcct gccggatcgt acagacccgc attgtcgtaa ccgcgggttt tcagctcagc
    16081 aacgcgctcg ctcgccagcg ttttcatcga ctctttcgta ccgaagtgaa tcgcgcccgt
    16141 tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc acgcggtcaa cgcacagcgt
    16201 acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg gacagcccgc
    16261 aatgcaataa ccgcagccaa tgcactgctc ggactggaag tcgacgatac cgttggcata
    16321 ctgaataatt gcaccttccg ccgggcacgc tttcaggcag cctggatcgg aacagtgcat
    16381 acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt ccgagaagcg
    16441 cattaccgtc cacgatttgg cgcttaaatc attggggttg tcgtacaccc caatgttatt
    16501 gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg ctttacagcc
    16561 gatacaggtg gtgacgtcga tgagtttcgc cacttcttcc tggaagtccc gcgcctgagg
    16621 cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgagatt gataagccat
    16681 atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact ccggcgtctg
    16741 cgtgttcgca tcaccaacga atggcgttaa cgtattggca ataaagcctt ttttagctac
    16801 gccttcataa ccccagtgaa tcggaatacc gatggtatcg atatctttgc cgttcgcttt
    16861 cagcgtacga atacgtttgg tcaccaccgc tttggctttg atatagccac ggttggagga
    16921 gactttcacg gtatcgccct gggcaatgcc aagtttattc gccagcgact ccccgatttc
    16981 cacaaactgc tctggttgca aaatcgcgtt caacagcgcg tgtttggtcc agtagtggaa
    17041 gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt tacccaatgc
    17101 ttcggcgtcg tctttaaaga tacgtgcagc cgggttcgag ataacgtttg gatgcagcgg
    17161 gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac cttccgccat
    17221 cttatcgagg gcaaacagac gccccatgcc ttcctgctgc atgataaacg gcccgacgcc
    17281 gctacccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact tagtgccgtc
    17341 ccatttcagc aactgacgct tcggatccca cgggttaccc tgcggatctg cggaggcgcg
    17401 gttatacaga atgcggcggt taagcggcca tgcccatgcc cagcccagcg tgttaccgag
    17461 gccagacgga tcggcgttat cacgacgcgc catctggttg ccttccggcg tccagctacc
    17521 ggcgaaaatc cagcagccac aggaggttgt accgtcatcg cgcaattggg cgaacgagct
    17581 aagttgttgg cctttcttga cgataaccgc accggttgcc ggatcggtaa tatcggccag
    17641 cgccttaccg ttgctctcca tcgctacttc ttccgacttc ggctcatgcg gaatggcgta
    17701 gttccaggtc atgttcagca cctggtccgg gttcgcgcca ccctgttcgg catacatctt
    17761 gcgcaagcgc aggaagatac cggagaggat ctcgccatca gtcagcgcaa tccccggggc
    17821 gtccgcacct ttccagtgcc actgcaacca gcgacctgag ttaacgattg aaccgttctc
    17881 ttctgcgaag caggtcgatg gcagacggaa cacttcggtc tggatcttcg acgagtcaac
    17941 ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca gtgggtcgat
    18001 ggtgacgagg aacttcagtt tcgacagaca accgatcact ttgtttttgt tcgggaatga
    18061 ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccctctt tcatcatctc
    18121 gaagtactgc aggacgtcgt agcctttatc ccacttcggc aaccagtcaa agccccagct
    18181 attttccgcc gtcgctttat caccaaagaa ggccttcatc atagagacga agaatttcgg
    18241 gtagttgccc cagtagttta cctggccttc cagcagcggt tttggcgtgt tggcggtaag
    18301 gtaggtttgc agatcggtct gcttctcgct tggcagcgtc atgtaacctg gcagactctg
    18361 cgacagtagc cccaggtccg tcagcccctg aatattggag tgaccgcg
//
LOCUS       DAFATA010000089        18373 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFATA010000089 DAFATA010000000
VERSION     DAFATA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.090"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(265..1302)
                     /gene="iap"
                     /locus_tag="LGJ20_003598"
     CDS             complement(265..1302)
                     /gene="iap"
                     /locus_tag="LGJ20_003598"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBK2387612.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTASDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSQGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRFDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            1554..2462
                     /gene="cysD"
                     /locus_tag="LGJ20_003599"
     CDS             1554..2462
                     /gene="cysD"
                     /locus_tag="LGJ20_003599"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBK2387613.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            2464..3891
                     /gene="cysN"
                     /locus_tag="LGJ20_003600"
     CDS             2464..3891
                     /gene="cysN"
                     /locus_tag="LGJ20_003600"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBK2387614.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARIDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            3891..4496
                     /gene="cysC"
                     /locus_tag="LGJ20_003601"
     CDS             3891..4496
                     /gene="cysC"
                     /locus_tag="LGJ20_003601"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBK2387615.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            4546..4869
                     /locus_tag="LGJ20_003602"
     CDS             4546..4869
                     /locus_tag="LGJ20_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBK2387616.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            5063..5374
                     /gene="ftsB"
                     /locus_tag="LGJ20_003603"
     CDS             5063..5374
                     /gene="ftsB"
                     /locus_tag="LGJ20_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBK2387617.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            5393..6103
                     /gene="ispD"
                     /locus_tag="LGJ20_003604"
     CDS             5393..6103
                     /gene="ispD"
                     /locus_tag="LGJ20_003604"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBK2387618.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQIPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            6103..6582
                     /gene="ispF"
                     /locus_tag="LGJ20_003605"
     CDS             6103..6582
                     /gene="ispF"
                     /locus_tag="LGJ20_003605"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBK2387619.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            6579..7628
                     /gene="truD"
                     /locus_tag="LGJ20_003606"
     CDS             6579..7628
                     /gene="truD"
                     /locus_tag="LGJ20_003606"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBK2387620.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            7609..8370
                     /gene="surE"
                     /locus_tag="LGJ20_003607"
     CDS             7609..8370
                     /gene="surE"
                     /locus_tag="LGJ20_003607"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBK2387621.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            8364..8990
                     /gene="pcm"
                     /locus_tag="LGJ20_003608"
     CDS             8364..8990
                     /gene="pcm"
                     /locus_tag="LGJ20_003608"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBK2387622.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            9130..10269
                     /gene="nlpD"
                     /locus_tag="LGJ20_003609"
     CDS             9130..10269
                     /gene="nlpD"
                     /locus_tag="LGJ20_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBK2387623.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            10332..11324
                     /gene="rpoS"
                     /locus_tag="LGJ20_003610"
     CDS             10332..11324
                     /gene="rpoS"
                     /locus_tag="LGJ20_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBK2387624.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(11445..11852)
                     /locus_tag="LGJ20_003611"
     CDS             complement(11445..11852)
                     /locus_tag="LGJ20_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK2387625.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            11999..12592
                     /locus_tag="LGJ20_003612"
     CDS             11999..12592
                     /locus_tag="LGJ20_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK2387626.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALRDMPNVETHLVMSKWAKTTIE
                     LETPYSAHDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            12592..14019
                     /locus_tag="LGJ20_003613"
     CDS             12592..14019
                     /locus_tag="LGJ20_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK2387627.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            14030..14266
                     /locus_tag="LGJ20_003614"
     CDS             14030..14266
                     /locus_tag="LGJ20_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387628.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(14305..15669)
                     /locus_tag="LGJ20_003615"
     CDS             complement(14305..15669)
                     /locus_tag="LGJ20_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBK2387629.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(15758..16534)
                     /locus_tag="LGJ20_003616"
     CDS             complement(15758..16534)
                     /locus_tag="LGJ20_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBK2387630.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(16539..17177)
                     /locus_tag="LGJ20_003617"
     CDS             complement(16539..17177)
                     /locus_tag="LGJ20_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBK2387631.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(17174..18322)
                     /locus_tag="LGJ20_003618"
     CDS             complement(17174..18322)
                     /locus_tag="LGJ20_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBK2387632.1"
                     /translation="MPEAIDALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFK
                     YCSTFDSTAKGNIGPVTDALMDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESG
                     MRHHPVNPMTDSYLPRLVEAQSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVL
                     DALTEHHLEIQGEALRDAPLVTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLS
                     GSCSQMTNRQVAHYRQIAPAREVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFA
                     TASTDALAAIQQQYGAQKASQAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLG
                     IKGFHIGPTISPGVPWVNALDKPVSLALKSGNFGDEAFFSRAQREFLS"
BASE COUNT         4396 a         4734 c         4988 g         4255 t
ORIGIN      
        1 ggcaaatata aattccagcg tgcttcatga agtgttcccc gcgccagcgg ggataaaccg
       61 gggcaaatat aaattccagc gtgcttcatg aagtgttccc cgcgccagcg gggataaacc
      121 gctgcgtagc gacctttgct ctcaatttcg ttggtgttcc ccgcgccagc ggggataaac
      181 cgggcgcact ggatgcgatg atggatatca cttggagttc ccccgcccct gcggtagaac
      241 tcccagctcc cattttccaa cccatcaaga cgccttcgcc aactctttca ccagaggtag
      301 cattatccgc ataacgtcac ggcagcgacg ttctattctt ccaggaagtg ccttatcaat
      361 atgctgttga ttatcaaacc tgacgtcatg ccagctattt cccgcaggga atgcggctgt
      421 ttttgcgcgt tgctgataac catccttatt cccaagattc cagttagtcg cttccaccga
      481 aagtacagca atgcccgctt tgtcgaatat ttctgcgtca ttacaacacc cggtgccttt
      541 cggatagttt ttattcaaac caggattggt cgtcgcggct attccctgac tgcgcgcaat
      601 tgccagtgcc ctgtcgcgcg ttaatttcct tactgcttca ggggttttta caccgctgtt
      661 gaaatacaat ttatcgccaa caattaagtt atcgagatta atcaccagca gcgtattttt
      721 cttttcggtg tcactcatcc gcttgagtaa attctcagcg cctaatttcc cttcctcttc
      781 gccgctggtc gccacaaatc gaataccata ctcggtaggc gtatttttca ggcgttctgc
      841 caattccagc atgacaccta aacctgcggc gttatcatcc attccttgta acgtcagccc
      901 gccgagattg gcatcggcgt cagcatcgct cagcggggcg taagtatcca gatgcgccat
      961 aatgatgatc tgctgcggtg ctttgccttc atgagcggca atcaccgtac ttcccgtcac
     1021 gttatgccag ctcttacgat tatcgctggc ggtataaata taccgactat taaatgtccg
     1081 aatatcactg cgataaccca tttgctgaaa ctgttggcga atataatcgg cagataacat
     1141 ttctgcagga gttccggtca tgcgtcccgg aaagaaagta gcaatatgtc gtgcctgggt
     1201 attagcaaaa tcgccaggtt taggtgacga ggcgtgtacg gggagaataa agcatacgcc
     1261 gagcgccagg gcagcggtac ggtggcgcaa tgcggaaaac atagtgagtc cttaaatacc
     1321 atgcaaattt ttttaccgcc atagtatgaa actgccgctg cgctaaaaca atttcaaatc
     1381 ttcctaaacg cccgaaatcc ggtgccttaa gcactttttg atattagctt tgccaaatcg
     1441 ttattccgtt aaggaactac tcattctaat tggtaatttc attcgttctc ttacgctccc
     1501 tatagtcgaa acatctgatg gcaagaaaat agcggtattg caaaggaacg gttatggatc
     1561 aaatacgact tactcacctg cggcaactgg aggcggaaag catccacatt attcgcgagg
     1621 tggcggcaga attctcaaat ccggtgatgc tctactctat cggtaaagat tccagcgtca
     1681 tgctgcatct ggcgcgcaag gcgttttatc caggtacgct gcctttcccg ttgctgcatg
     1741 tcgataccgg ctggaaattc cgcgagatgt atgagttccg cgatcgtacg gcgaaagcct
     1801 acggctgcga gctgctggtg cataaaaacc cggaaggcgt ggcgatgggg attaatccat
     1861 tcgtgcacgg cagcgcgaaa cataccgata ttatgaaaac tgaaggcctg aaacaggcgc
     1921 tgaacaaata cggttttgat gccgccttcg gtggtgcgcg ccgtgacgaa gagaaatccc
     1981 gcgctaaaga gcgaatctac tctttccgtg accgcttcca tcgctgggat ccgaaaaatc
     2041 agcgcccgga gctgtggcac aactacaacg ggcaaattaa caaaggcgaa agcatccgcg
     2101 tcttcccgct ctctaactgg accgagcagg atatctggca atacatctgg ctggaaaata
     2161 tcgacattgt gccgctgtat ctcgctgcgg aacgtccggt tctggaacgc gacggtatgt
     2221 tgatgatgat tgatgacaac cgtatcgacc tgcaaccggg cgaagtgatt aaaaaacgga
     2281 tggtgcgttt ccgtacgctg ggctgctggc cgctgaccgg tgcggtggag tcaaatgcac
     2341 aaacactgcc ggagatcatc gaagagatgc tggtttccac caccagtgaa cgtcagggcc
     2401 gcgtgattga ccgcgaccag gcggggtcta tggagctgaa aaaacgtcag gggtattttt
     2461 aagatgaaca ccgcacttgc acaacaaatc gctaatgaag gcggcgtcga agcctggatg
     2521 attgcgcaac aacataaaag cctgctgcgt tttctgacct gtggtagcgt cgatgacggc
     2581 aaaagtaccc tgattggtcg cctgctgcac gatacccgcc aaatctacga agatcagctc
     2641 tcatcgctgc ataacgacag taagcgtcac ggcacccagg gcgaaaaact ggatctggct
     2701 ctgctggtgg acggcctgca agctgagcgc gaacagggca tcactattga tgtggcctac
     2761 cgctatttct ctaccgagaa gcgtaaattt attatcgccg acacccccgg gcacgagcag
     2821 tatacccgca atatggcgac cggcgcctca acgtgtgagc tggcgatctt actgatcgat
     2881 gcccgtaaag gcgtgctcga tcaaacccgt cgtcacagtt ttatctccac gctgctgggg
     2941 atcaaacatc tggtcgtagc gatcaacaaa atggatctgg tggattacag tgaagagaca
     3001 ttcacccgca ttcgtgaaga ctacctgact ttcgccgaac agttacctgg caatctggat
     3061 attcgctttg tgccgctctc tgcgctggaa ggcgacaacg tggcttcgca aagtgaaagt
     3121 atgccgtggt acagcggtcc gacactgctc gaagtgctgg aaaccgtgga gatccagcga
     3181 gtggtggatg ctcagccaat gcgcttcccg gtgcagtacg ttaatcgccc gaatctcgat
     3241 tttcgtggct acgccggaac gctggcatcc ggtcgcgtgg aagtcgggca acgtgtcaaa
     3301 gtgctgccct ctggtgtgga atcaaacgtc gcgcggatcg tgacttttga tggtgatcgc
     3361 gaagaagcct ttgccggaga agcgatcacc ctggtgctga cggatgagat cgacatcagc
     3421 cgtggcgatc tgctgctggc ggcagacgaa gcgttaccgg cggtgcagag cgcgtcggtg
     3481 gatgtggtat ggatggcgga acagccgctt tccccgggcc agagttacga catcaaaatt
     3541 gccggtaaga agacgcgcgc gcgtattgat ggcattcgct atcaggttga tattaataac
     3601 cttacccagc gtgaagttga aaacctgcca ctgaatggga tcggcctcgt ggatctcact
     3661 tttgacgagc cgctggtgtt agatcgttat caacaaaatc cggtgacggg tgggctgatt
     3721 tttatcgatc gcctgagcaa tgtgaccgtg ggtgccggta tggtgcacga gccagttagc
     3781 caggcaactg ctgcgccatc tgaattcagt gcattcgaac tggaattgaa tgctctggtt
     3841 cgtcgccact ttccgcactg gggcgcgcgc gatttgctgg gggataaata atggcgctgc
     3901 atgacgaaaa cgtcgtctgg catagccatc cggtcactgt gcaacaacgc gagctacacc
     3961 acggtcatcg tggtgtagtg ctgtggttta ccggcctctc cgggtccggt aaatcaacgg
     4021 tcgccggggc gctggaggag gcgttacata aactcggcgt cagtacgtat ctgctggatg
     4081 gcgataatgt tcgccacgga ttatgcagcg atctcggttt tagcgatgcc gatcgtaaag
     4141 agaatatccg tcgcgtcggt gaagtggcga atttgatggt tgaagccgga ctggtagtgc
     4201 tgaccgcatt tatctcgcca caccgcgccg aacgccagat ggttcgcgaa cgcgtaggag
     4261 aagggcgctt tatcgaagtg tttgtcgata cgccgctggc gatttgcgaa gcccgcgatc
     4321 caaaaggctt atataagaaa gcgcgtgccg gtgaactgcg caactttacg ggaatagatt
     4381 ccgtttacga agcgcctgaa tcggcagaaa ttcatctcaa tggtgaacaa ttagtaacaa
     4441 atttggtaca gcaattatta gatctgttga gacagaacga tattatcaga tcctgagaca
     4501 ccaccgggtt tccatgcccg gttcgtcaaa gtaacaggat caaatatgcg taatagccat
     4561 aacattacac taacaaataa cgacagcctt actgaggatg aagaaaccac atggtcactg
     4621 cctggtgccg tggtcggttt tatctcctgg ttatttgcgc tggcgatgcc gatgttgatt
     4681 tatggctcta acacgctgtt cttctttatc tacacctggc ctttctttct ggcgctgatg
     4741 cccgtcgcgg tagtggtggg gattgcgctg cattcattga tggacggaaa gctacgctac
     4801 agtattgttt tcaccctggt gacagtaggc attatgtttg gcgcactgtt tatgtggcta
     4861 ctgggctaat ttgtactttc ccgtctcctc tgttcataat tcaaaccgta actaataatg
     4921 agattatgtt ctgcacgccc tgggtatacg taacaatgga caaatgtggt acatttgccc
     4981 gcgttgtcgc ggtatcccca acagaggatg tagagtcgtc ttcggatgca tgggatgatg
     5041 atgccgtttt tcagggggca ggatgggtaa actaacgctg ctgttgctgg ctattctggt
     5101 ctggctacag tattcgctgt ggttcggtaa gaacggtata catgactata cccgcgtcaa
     5161 tgatgatgtg gcggcacagc aagctacaaa cgcgaaactt aaagcgcgaa acgatcaact
     5221 ttttgccgaa attgacgatc tcaatggcgg ccaggaggcg ctcgaagagc gtgcgcgtaa
     5281 tgaactcagc atgaccaggc cgggcgaaac tttttatcgt ctggtgcctg acgcgtcgaa
     5341 gcgcgcacag tctgcggggc aaaacaatcg ataaatcagc ccgggaatta acatggcaac
     5401 cactcatttg gatgtttgcg ccgtggttcc ggcggccgga tttggccgtc gaatgcaaac
     5461 ggaatgtcct aagcaatatc tctcaatcgg taatcaaacc attcttgaac actcggtgca
     5521 tgcgctgctg gcgcatcccc gggtgaaacg tgtcgtcatt gccataagtc ctggcgatag
     5581 ccgttttgca caaattcctc tggcgaatca tccgcaaatc accgttgtag atggcggtga
     5641 tgagcgtgcc gattccgtgc tggcaggtct gaaagccgct ggcgacgcgc agtgggtatt
     5701 ggtgcatgac gccgctcgtc cttgtctgca tcaggatgac ctcgcgcgat tgttggcgtt
     5761 gagcgaaacc agccgcacgg gagggatcct agccgcacca gtgcgcgata cgatgaaacg
     5821 tgccgaaccg ggcaaaaatg ccattgctca taccgttgat cgcaacggct tatggcacgc
     5881 gctgacgccg caatttttcc ctcgtgagct gttacatgac tgtctgacgc gcgctctaaa
     5941 tgaaggcgcg actattaccg acgaagcctc ggcgctggaa tattgcggat tccatcctca
     6001 gttggtcgaa ggccgtgcgg ataacattaa agtcacgcgc ccggaagatt tggcactggc
     6061 cgagttttac ctcacccgaa ccatccatca ggagaataca taatgcgaat tggacacggt
     6121 tttgacgtac atgcctttgg cggtgaaggc ccaattatca ttggtggcgt acgcattcct
     6181 tacgaaaaag gattgctggc gcattctgat ggcgacgtgg cgctccatgc gttgaccgat
     6241 gcattgcttg gcgcggcggc gctgggggat atcggcaagc tgttcccgga taccgatccg
     6301 gcatttaaag gtgccgacag ccgcgagctg ctacgcgaag cctggcgtcg tattcaggcg
     6361 aaggggtata ccctgggcaa cgtcgatgtc actatcatcg ctcaggcacc gaagatgttg
     6421 ccgcacattc cacaaatgcg cgtgtttatt gccgaagatc tcggctgcca tatggatgat
     6481 gttaacgtga aagccactac tacggaaaaa cttggattta ccggacgtgg ggaagggatt
     6541 gcctgtgaag cggtggcgct actcattaag gcaacaaaat gattgagttt gataatctca
     6601 cttacctcca cggtaaaccg caaggcaccg ggctgctgaa agccaatccg gaagactttg
     6661 tggtggtgga agatttgggc tttgagcctg atggtgaagg tgagcatatt ctggttagaa
     6721 tcctcaaaaa cggctgcaat acccgttttg tggcggatgc actggcgaaa ttcctgaaaa
     6781 ttcatgcccg tgaagtcagc ttcgctgggc aaaaagacaa acatgctgtt acggaacagt
     6841 ggttatgcgc tcgcgtgcca ggcaaggaaa tgcccgatct gagcgccttt caactggaag
     6901 gctgccaggt gctggagtat gcgcggcaca agcgcaagct gcgtttaggc gcgctgaaag
     6961 gtaacgcctt taccctggtt ctgcgcgaag tgagcaatcg cgatgacgtt gaacaacgtc
     7021 tgatcgatat ttgcgtaaaa ggtgtaccga actacttcgg tgcccaacgt tttgggattg
     7081 gcggtagcaa cttgcagggg gcgcagcgct gggcgcaaac caatactccg gtgcgcgatc
     7141 gcaataaacg gagtttttgg ttgtcggcag cccgcagtgc gttgtttaat cagattgttg
     7201 ctgagcgcct caaaaaagca gacgttaatc aagttgttga cggcgatgcg ctacaattag
     7261 ccggacgtgg tagctggttt gtcgcaacca ccgaagaact ggcggaatta cagcgtcgcg
     7321 ttaacgataa agagctgatg ataaccgccg cattgccggg cagtggcgaa tggggaactc
     7381 agcgtgaagc gctggcattc gaacaagcag ctgttgccgc agaaactgaa ttacaagctt
     7441 tactggtgcg cgaaaaagtt gaagccgcgc gcagagcgat gctgctgtat ccgcaacaat
     7501 taagctggaa ttggtgggat gacgtcaccg tagagatccg tttctggctt ccggcgggta
     7561 gttttgcaac cagcgttgtc agggaactta tcaacacaac aggtgattat gcgcatattg
     7621 ctgagtaatg atgacggggt acatgcaccc ggtatacaaa cgctggcgaa agccttgcgt
     7681 gagtttgctg acgttcaggt ggtcgccccc gatcgtaacc gcagcggcgc ttcaaattct
     7741 ctgacactgg aatcctccct gcgcacgttt acctttgaaa atggtgatat tgctgtgcaa
     7801 atgggaaccc cgaccgattg cgtctatctt ggcgtgaatg ctctgatgcg tccgcgcccg
     7861 gacattgttg tgtccggaat taacgccggg ccgaatctgg gggatgatgt tatttattcc
     7921 ggtacggtag ccgccgcgat ggaaggccgt catttaggtt ttccggcgct tgccgtctcg
     7981 cttgacgggc ataaacatta cgacactgcc gcggcggtaa cctgttcaat tttgcgcgca
     8041 ctgtgtaaag agccgttgcg caccgggcgt attcttaata ttaacgttcc ggatttaccc
     8101 ttggatcaaa tcaaaggtat tcgcgtgacg cgctgcggta cacgacatcc ggcagatcag
     8161 gtgatcccgc agcaagatcc gcgcggcaat acgctgtact ggattggccc gccgggcggt
     8221 aaatgtgatg ccggtccggg gaccgatttt gctgcggtag atgagggcta tgtctccatc
     8281 acgccgctgc atgtggattt aactgcgcat agcgcgcaag atgtggtttc agactggtta
     8341 aacagcgtgg gagttggcac gcaatggtaa gcagacgcgt acaagcactt ctggatcaat
     8401 tacgtgcgca aggtattcag gatgagcagg tgctgaatgc acttgctgcc gtgccgcgtg
     8461 aaaaattcgt tgatgaagcg tttgaacaaa aagcctggga caatatcgcc ttgccgatag
     8521 gtcaggggca gacaatttcg cagccgtata tggtggcgcg aatgaccgaa ttactcgagc
     8581 tgacgccgca gtcgcgggtg ctggaaattg gcaccggttc gggatatcaa acggcaatcc
     8641 tggcgcatct tgtccagcat gtttgctcgg tcgaacggat taaaggtttg cagtggcagg
     8701 cgcgtcgccg cctgaaaaat cttgatttac ataatgtttc aacccgtcat ggcgatggat
     8761 ggcaaggttg gcaggcacgc gcgccgtttg acgctatcat tgttacggcg gcaccgccgg
     8821 aaattccaac tgcgctaatg acgcagctgg acgaaggcgg gattctcgtc ttacccgtag
     8881 gggaggagca ccagtatttg aaacgggtgc gtcgtcgggg aggcgaattt attatcgata
     8941 ccgtggaggc cgtgcgcttt gtccctttag tgaagggtga gctggcttaa aacgtgagga
     9001 aatacctgga tttttcctgg ttattttgcc gcaggtcagc gtatcgtgaa catcttttcc
     9061 agtgttcagt agggtgcctt gcacggtaat tatgtcactg gttattaacc aatttttcct
     9121 gggggataaa tgagcgcggg aagcccaaaa ttcaccgttc gccgcattgc ggctttgtca
     9181 ctggtttcgc tatggctggc aggctgttct gacacttcaa atccaccggc accggtcagc
     9241 tccgttaatg gcaatgcgcc tgcaaatacc aattctggta tgttgattac gccgccgccg
     9301 aaaatgggga cgacgtctac agcccagcaa ccgcaaattc agccggtaca gcagccacaa
     9361 attcaggcta ctcaacaacc gcaaatccag ccagtacagc cagtagctca gcagccggta
     9421 cagatggaaa acggacgcat cgtctataac cgtcagtatg ggaacattcc gaaaggcagt
     9481 tatagcggca gtacctatac cgtgaaaaaa ggcgacacac ttttctatat cgcctggatt
     9541 actggcaacg atttccgtga ccttgctcag cgcaacaata ttcaggcacc atacgcgctg
     9601 aacgtcggtc agaccttaca ggtgggtaat gcttccggta cgccaatcac tggcggaaat
     9661 gccattaccc aggccgatgc agcagagcaa ggagttgtga tcaagcctgc acaaaattcc
     9721 accgttgctg ttgcgtcgca accgacaatt acgtattctg agtcttcggg tgaacagagt
     9781 gctaacaaaa tgttgccgaa caacaagcca actgcgacca cggtcacagc gcctgtaacg
     9841 gtaccaacag caagcacaac cgagccgact gtcagcagta catcaaccag tacgcctatc
     9901 tccacctggc gctggccgac tgagggcaaa gtgatcgaaa cctttggcgc ttctgagggg
     9961 ggcaacaagg ggattgatat cgcaggcagc aaaggacagg caattatcgc gaccgcagat
    10021 ggccgcgttg tttatgccgg taacgcgctg cgcggctacg gtaatctgat tatcatcaaa
    10081 cataatgatg attacctgag tgcctacgcc cataacgaca caatgctggt ccgggaacaa
    10141 caagaagtga aggcggggca aaaaatagca accatgggta gcaccggaac cagttcaaca
    10201 cgcttgcatt ttgaaattcg ttacaagggg aaatccgtaa acccgctgcg ttatttgccg
    10261 cagcgataaa tcggcggaac caggcttttg cttgaatgtt ccgtcaaggg atcacgggta
    10321 ggagccacct tatgagtcag aatacgctga aagttcatga tttaaatgaa gatgcggaat
    10381 ttgatgagaa cggagttgag gtttttgacg aaaaggcctt agtagaagag gaacccagtg
    10441 ataacgattt ggccgaagag gaactgttat cgcagggagc cacacagcgt gtgttggacg
    10501 cgactcagct ttaccttggt gagattggtt attcaccact gttaacggcc gaagaagaag
    10561 tttattttgc gcgtcgcgca ctgcgtggag atgtcgcctc tcgccgccgg atgatcgaga
    10621 gtaacttgcg tctggtggta aaaattgccc gccgttatgg caatcgtggt ctggcgttgc
    10681 tggaccttat cgaagagggc aacctggggc tgatccgcgc ggtagagaag tttgacccgg
    10741 aacgtggttt ccgcttctca acatacgcaa cctggtggat tcgccagacg attgaacggg
    10801 cgattatgaa ccaaacccgt actattcgtt tgccgattca catcgtaaag gagctgaacg
    10861 tttacctgcg aaccgcacgt gagttgtccc ataagctgga ccatgaacca agtgcggaag
    10921 agatcgcaga gcaactggat aagccagttg atgacgtcag ccgtatgctt cgtcttaacg
    10981 agcgcattac ctcggtagac accccgctgg gtggtgattc cgaaaaagcg ttgctggaca
    11041 tcctggccga tgaaaaagag aacggtccgg aagataccac gcaagatgac gatatgaagc
    11101 agagcatcgt caaatggctg ttcgagctga acgccaaaca gcgtgaagtg ctggcacgtc
    11161 gattcggttt gctggggtac gaagcggcaa cactggaaga tgtaggtcgt gaaattggcc
    11221 tcacccgtga acgtgttcgc cagattcagg ttgaaggcct gcgccgtttg cgcgaaatcc
    11281 tgcaaacgca ggggctgaat atcgaagcgc tgttccgcga gtaagtaagc atctgtcaga
    11341 aaggccagtc tcaagcgagg ctggcctttt ttctttcggt tggtgagtcg gtgtatttgt
    11401 agtctggata agacgcgata gcgtcgcatc cggaaatttg ccccctatgc gttcatcatc
    11461 ttgcgcacca gctgcataaa cagctcctgc tcttctccac tcaaacgccc caaaaattcc
    11521 tcatccacgc tgtcaccaat gggtatcgcc gcagcaagta tcttttcgcc ttcggcagtc
    11581 aaccagacaa agcgtcgccg cttatcagcg gggtcatgtt cacgtctgac taagccacga
    11641 ttttccattc ttgccaacat ttctgctaac gttgctttgg tgctgacagc tgcttctatc
    11701 agtgccacct gttcgatacc aggtttatca gcaatggcgc gcatcaccgc gtactgtggt
    11761 ttggtcaggt caggtaactc gtgctgccag cgagccgtgt gctgctgaaa aagctgtcgt
    11821 aactgatgga acgctttatt tcgtaacgcc atgtaaactc cgggctaatt ctctgtcgct
    11881 atcatagcgg ttttttagtg acgaagaaga tgttgttact tttcaatcct tgccgctgtg
    11941 gaaatgaagg agtatgttaa taataacgtt cgtatacgaa caattaagag gataagcaat
    12001 gaaactgatc gtcgggatga caggggctac cggtgcgcct cttggtgtgg cattactgca
    12061 agcgctgcgg gatatgccga atgtcgagac tcatctggtg atgtcgaagt gggcgaaaac
    12121 caccattgaa ctagaaacgc cttacagcgc tcacgatgtt gctgccctcg cagacttcag
    12181 ccataacccg gcggatcagg cggcgatcat ctcatccggt tcttttcgta ccgacggcat
    12241 gatcgttatt ccgtgcagta tgaaaacgct cgccggtatc cgcgctggtt acgccgatgg
    12301 cctggtaggg cgcgcggcgg acgtcgtgct caaagaaggc cgcaaactgg tgctggtgcc
    12361 gcgtgaaatg ccgcttagca ccatccatct cgaaaatatg ctcgcacttt cacgcatggg
    12421 cgtggcgatg gtgccgccga tgcctgcctt ttataaccat cccgaaacgg tagatgacat
    12481 tgtccaccat gtggtagccc gcgtgctgga tcaatttggc ctcgaacatc cccatgccag
    12541 gcgctggcaa ggattgccgc aggcccggaa tttttctcag gagaatgaat aatggcattt
    12601 gatgatttac gcagcttttt acaggcgctt gatgaccacg gccagttact gaaaatcagc
    12661 gaagaggtga acgctgagcc ggatctggca gcagcagcta acgccaccgg gcgtatcggc
    12721 gacggcgcgc ccgcgctgtg gtttgataat attcgcggct ttaccgatgc ccgcgtgacg
    12781 atgaatacca tcggttcctg gcagaaccac gcgatttccc ttggcctgcc accaaacacc
    12841 ccggtcaaaa agcagattga tgagtttatc cgccgctggg ataacttccc gattgccccg
    12901 gagcgccgcg ccaatccagc ctgggcgcag aacaccgtcg atggcgaaga gattaacctg
    12961 ttcgatatcc tgccgctgtt tcgtttaaac gatggcgatg gcggtttcta tctcgacaaa
    13021 gcctgcgtgg tttcccgcga tccgcttgac ccagataact tcggcaagca gaacgtcggc
    13081 atctaccgca tggaagtgaa gggcaagcgt aagctcggcc tgcaaccggt gccgatgcac
    13141 gatatcgccc tgcatctgca taaagcagaa gagcgcggtg aagatctgcc gattgcgatc
    13201 acgctcggta acgatccgat catcacgctg atgggggcca cgccgctgaa atacgatcag
    13261 tctgagtatg aaatggcagg cgcgctgcgt gaaagcccgt acccgatcgc caccgccccg
    13321 ctgaccggtt ttgatgtgcc gtggggttca gaagtgatcc tcgaaggggt tatcgaaagc
    13381 cgtaaacgcg aaatcgaagg gccgttcggt gagtttaccg ggcactactc cggcgggcgt
    13441 aacatgaccg tggtgcgcat cgataaagtc tcttaccgca ccaggccgat tttcgaatcg
    13501 ctgtacctcg gtatgccgtg gaccgaaatc gactacctga tggggccagc cacctgcgtg
    13561 ccgctgtatc agcagctgaa agccgagttc ccggaagtgc aggcggtaaa cgccatgtac
    13621 acccatggcc tgctggcgat tatctccacc aaaaaacgct acggcggctt tgcccgcgcg
    13681 gtgggcctgc gcgcaatgac cacgccgcat ggtctgggct acgtgaagat ggtgattatg
    13741 gtcgatgaag acgttgaccc gttcaacctg ccgcaggtga tgtgggcgct ctcctcgaaa
    13801 gtgaacccgg caggggattt ggtgcagttg ccgaatatgt ccgtgctgga actcgatcca
    13861 ggctcaagcc ctgcggggat caccgacaag ctgattatcg acgccactac gcctgtcgcc
    13921 ccggacaacc gtggtcacta cagccaaccg gtggtggatt taccggaaac caaagcctgg
    13981 gctgaaaaac tgaccgctat gctggctgca cgtaaataag gagaagaaga tgatttgtcc
    14041 acgttgtgcc gatgaacaga ttgaagtgat ggcgaaatcg ccggtgaaag atgtctggac
    14101 ggtatatcag tgccagcatt gcctttatac ctggcgcgat accgaaccgc tgcgccgtac
    14161 cagccgcgaa cattatcccg aagcgttccg catgacgcag aaagatattg atgacgcgcc
    14221 aatggtgccg agcatcccgc cgctgctggt ggaaggtaag cgttaaataa aaggtccgat
    14281 gcccatcgga cctttttatt aaggtcaaat taccgcccat acgcaccagg taattaagaa
    14341 tccggtaaaa ccgagaatgg tcgttaacac tgtccaggtt ttcagaccgt ctgctaccga
    14401 caaccccaga tatttggtca caatccagaa ccctgagtca ttaatatgtg acgcgccaag
    14461 cccaccaaag caggctgcca gcgtcaccaa tacgcactga atcggattca atcccatcac
    14521 cgcttctgag agtaacccgc cggttgtcag gattgctacg gttgctgacc cctgcgatgc
    14581 acgcagagcc agtgaaataa taaatgcggc tggtaacaga ggcaggtcaa tcatttgtag
    14641 catattggca agggctttgc cgacgcccga ttccaccagc actttgccaa atacccctcc
    14701 agcaccagta accaaaatca ctaccgccgc agtgggaagg gctgagccca taatgtcgct
    14761 ggtgtgttgt aagctccagc cgcgacgtaa agccaataac cagaatgcca gcaccagcgc
    14821 aatcattaga gctaccattg gtgagccgat cagctgtagc gtaccaagca ggggatgcga
    14881 aggcggcatc agtgttgcgg aaaccgtacc cgccatgata atcgcgatag gaataacaat
    14941 tagcgaggtg accagcgcga cgcccggtgg atttatttta tcgcttaatt ttgttgcgcc
    15001 ttcctcactg gccggagcca gttgcatctg ttccagtact tccaccgaca tcgcatattg
    15061 gcgcgtattg attattttcg ctgcaaagta gccaacaatc cctacgggaa tagaaatcgc
    15121 aataccgatg atggttagcc agccgatgtc tgcgtggagt aaccccgctg cggcgacagg
    15181 gcctggatgc ggcggtaccg ccacatgaac agttagcatg attccagcga caggcaggcc
    15241 aaatttgagt ggcgatattt tggcaacctt ggcaaaaccg taaatgattg gcgcaagaat
    15301 aataaagccg acatcaaaga agacgggaat accgaggaag aacgctgcca gagtcagcgc
    15361 agcgatagtt cgtttgtcac ctaacttgcg actgaaataa ttagccagtg actctgcacc
    15421 accagagtgt tcgatcatac gccccagcat agcgcccaga ccaataataa tagtgacgga
    15481 accaagcaca ccgcccatcc cggcgatcat cactttaccc acttcgcccg ccggtatacc
    15541 cgccgcaagt gcgactaaca ggctgacgag gagcagagca acgaatggtt gtacctttgc
    15601 cttgatgacc agcagcaaca gcatgattac gccagctaac gcaatgcata acaatgtaat
    15661 tgtggacatg ggaaaccctg tctgaaagtt atagttaacc taccccatcc gtagatgggg
    15721 ggatgtatgg gtacgttgta attagggatt taacgaatta gcgccaggcg tcaaaccagc
    15781 caagcccttc ttcggtgagg ccacgaggtt tatattcaca accgatccag ccctgatatc
    15841 ccacctcatc gaacaggcgg aacagccacg gatagttgat ttctccatcg tccggttcat
    15901 gtcgatcagg tagtccggca atttgtacgt gcgcatattt cccagcgtag tcgcggatta
    15961 aatgcgtcag gttgccatct actttttgcg catgaaaagt atctagttga ataaacacgt
    16021 tatctcgcgc aacctcttca acaatagcca gtgcctgata ctggctggag aagagataat
    16081 gaggcttaac gtcggggctg agtgcttcaa ctaatattcg cttgccgtgt agcgcaaagc
    16141 ggtcggcagc gtagcggaga ttatcgataa atactgcccg gtaccgttca gcgtcttcgc
    16201 cagcgggcac gacgcctgcc atcacatgga cttgttcaca attgagcgcc aatgcatatt
    16261 ccagtgccag gtcgatgtct gcgtgtgctt cgtgctcacg tccgggaagg gcggataatc
    16321 cccattcccc cgcattaata tctccgggag cggtattgaa cagcgccagt gtcagatggt
    16381 tttgctccag ttgcttttgg atttgcaggg tggagtagtc atagggaaac agaaattcca
    16441 cagcatcgaa cccggctttt cgcgctgcgg cgaagcgttc aataaaaggc acttcggtga
    16501 acatcatgga taaattagct gcaaaacgag gcattgcatt aactccttaa ttccgcaatt
    16561 tcacctgcgg tcagataacg gatcgggcgg tcaccgagaa taaaaatcag ctttgccgtt
    16621 tcctccagct cttccatatt gttggcggct tcttgcaggc tttcaccgca aaccactggg
    16681 ccatgatttg ccagtaaaaa agcctgattg tctgctgcca gttccgccag atcctgtgcg
    16741 atgcgtttat cgcccggtcg gtaataaggc accagcggga catttcccat ccgcatcacc
    16801 acgtatggtg tgaacggacg aataacgttg ctgctgtcca gcccttgcag gcaggaaagc
    16861 gccgtcgacc atgtgctgtg caaatgcacc accgctttac agcgcggatt gttgcgatac
    16921 agcgccagat gaaagagcac ctctttcgag ggtttgtcac cacttaacca ttcgccatcc
    16981 gcggcgactt tggaaagccg ctgcggatcg agattgccca ggcatgaacc tgtcggtgtc
    17041 gccagtaaat tcccgtcagg taaaagcagc gacagattgc cagccgaacc ggttgcatag
    17101 ccgcgctgaa agaatgaact ggcaatccgc gtcatctcct ctcgcaaaga ctgctctact
    17161 tttgcgaaat cgctcatgat aaaaactctc tttgggctcg tgaaaaaaaa gcttcatcac
    17221 cgaagttgcc agatttaagg gcgagtgaga caggcttatc cagtgcgttt acccacggca
    17281 cgccggggga aatggttggg ccaatatgaa acccttttat gcccaggctc tgtgtgacta
    17341 cgccggaggt ctcaccgcct gcgacaataa agcgtgtcac gccttccgct gctaaccgcg
    17401 ccgctagttg agaaaataga gtttctactg cctgactggc tttttgtgca ccgtattgct
    17461 gttgaattgc tgccaatgcg tcagtgctgg cggtggcaaa aaccagtgga gcaagtacac
    17521 tttcctggcc cagaacccac tctgccagtt cgtgtgcata agcggccaga gtttcggttg
    17581 agaggcagcg tgccacatca acttcacggg ctggtgcaat ttgacggtaa tgtgccacct
    17641 ggcggttggt catttgagag catgaaccgg agagcactac gccgcgccca gcgagcggat
    17701 gccctgcttc gcgagcctgg ttaccgtttt cttgcgccca ctgccgggcc aggccaatcg
    17761 ccagaccaga accgcccgtt accagtgggg catcgcgcaa ggcttctccc tgaatttcca
    17821 gatggtgttc ggtcagcgca tcaagcaccg cgtagcggta gccctcttgc tgtaagcgag
    17881 ccagctcttg acgaacggca tccacacctt gttcgaaaac atgtgccgaa acgacgccgc
    17941 agcgccctgt ggattgcgct tcaaccagac ggggaagata gctgtcggtc atgggattta
    18001 ccgggtgatg gcgcatcccg gattcggcca gcagttgatt cattacgaac aaatacccct
    18061 gataaaccgt acgtccgttg accggcaggg ccggagagaa gaccgtaaac ggcgtgtcga
    18121 gagcatccat taatgcatcg gtaaccgggc cgatattacc tttcgccgta ctgtcgaaag
    18181 tagagcagta tttgaaatag atctgtttgc aaccttgctg ttgcaaccag ctcagagccg
    18241 ccagcgattg ctgtgtggct tcaaccaccg gacaggagcg cgttttcagg ctgatcacca
    18301 gtgcgtcgat tgcttccggc attttacctg ttggaacacc gttaatttgt accgttggta
    18361 gaccgttttc cac
//
LOCUS       DAFATA010000090        18044 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFATA010000090 DAFATA010000000
VERSION     DAFATA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.091"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..744
                     /gene="corA"
                     /locus_tag="LGJ20_003619"
     CDS             <1..744
                     /gene="corA"
                     /locus_tag="LGJ20_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBK2387633.1"
                     /translation="DGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLFTLRERELPAFR
                     LYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSRVIMEGHQGDEY
                     DEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPH
                     NESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELK
                     WSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(798..1277)
                     /locus_tag="LGJ20_003620"
     CDS             complement(798..1277)
                     /locus_tag="LGJ20_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBK2387634.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1274..2122)
                     /locus_tag="LGJ20_003621"
     CDS             complement(1274..2122)
                     /locus_tag="LGJ20_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBK2387635.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQNKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQNYA
                     IGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
     gene            2279..2455
                     /locus_tag="LGJ20_003622"
     CDS             2279..2455
                     /locus_tag="LGJ20_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBK2387636.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            3079..3378
                     /locus_tag="LGJ20_003623"
     CDS             3079..3378
                     /locus_tag="LGJ20_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387637.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(3425..4312)
                     /gene="rarD"
                     /locus_tag="LGJ20_003624"
     CDS             complement(3425..4312)
                     /gene="rarD"
                     /locus_tag="LGJ20_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBK2387638.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(4364..4831)
                     /locus_tag="LGJ20_003625"
     CDS             complement(4364..4831)
                     /locus_tag="LGJ20_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBK2387639.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4996..5865
                     /gene="pldA"
                     /locus_tag="LGJ20_003626"
     CDS             4996..5865
                     /gene="pldA"
                     /locus_tag="LGJ20_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBK2387640.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5998..7827
                     /gene="recQ"
                     /locus_tag="LGJ20_003627"
     CDS             5998..7827
                     /gene="recQ"
                     /locus_tag="LGJ20_003627"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK2387641.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7891..8511
                     /gene="rhtC"
                     /locus_tag="LGJ20_003628"
     CDS             7891..8511
                     /gene="rhtC"
                     /locus_tag="LGJ20_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBK2387642.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(8573..9193)
                     /gene="rhtB"
                     /locus_tag="LGJ20_003629"
     CDS             complement(8573..9193)
                     /gene="rhtB"
                     /locus_tag="LGJ20_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBK2387643.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            9304..10326
                     /gene="pldB"
                     /locus_tag="LGJ20_003630"
     CDS             9304..10326
                     /gene="pldB"
                     /locus_tag="LGJ20_003630"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBK2387644.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            10334..11134
                     /gene="yigL"
                     /locus_tag="LGJ20_003631"
     CDS             10334..11134
                     /gene="yigL"
                     /locus_tag="LGJ20_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBK2387645.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            11210..12109
                     /gene="bioP"
                     /locus_tag="LGJ20_003632"
     CDS             11210..12109
                     /gene="bioP"
                     /locus_tag="LGJ20_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBK2387646.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11997..12950)
                     /gene="metR"
                     /locus_tag="LGJ20_003633"
     CDS             complement(11997..12950)
                     /gene="metR"
                     /locus_tag="LGJ20_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBK2387647.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            13068..15329
                     /gene="metE"
                     /locus_tag="LGJ20_003634"
     CDS             13068..15329
                     /gene="metE"
                     /locus_tag="LGJ20_003634"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK2387648.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(15368..16183)
                     /locus_tag="LGJ20_003635"
     CDS             complement(15368..16183)
                     /locus_tag="LGJ20_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBK2387649.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            16445..17206
                     /gene="udp"
                     /locus_tag="LGJ20_003636"
     CDS             16445..17206
                     /gene="udp"
                     /locus_tag="LGJ20_003636"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBK2387650.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            17347..>18044
                     /gene="rmuC"
                     /locus_tag="LGJ20_003637"
     CDS             17347..>18044
                     /gene="rmuC"
                     /locus_tag="LGJ20_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBK2387651.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIE"
BASE COUNT         4430 a         4334 c         4683 g         4597 t
ORIGIN      
        1 gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca cgccggtaac
       61 tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga gcgtgaactg
      121 cctgcattcc gtctgtatcg tatgcgcgcc cgtagccagt caatggtgga cggtaacgcc
      181 tacgagctgc tgttggatct gttcgaaacc aaaatcgaac agctggcaga tgaaattgaa
      241 aatatctaca gcgatctgga gcagttgagc cgggtaatta tggaagggca tcagggcgat
      301 gagtacgatg aggcgctctc cacgctggcg gaactggaag atatcggctg gaaagttcgc
      361 ctgtgtctga tggataccca gcgtgcgctc aacttcctgg tacgcaaggc gcgtttaccg
      421 ggtgggcaac tggagcaggc gcgtgaaatc ctgcgagata tcgaatccct gctgccgcat
      481 aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcgatggg ctttatcaac
      541 atcgagcaga accgcatcat caaaatcttc tcggtggtat ccgtggtatt cctgccgccg
      601 acgctcgttg cttccagcta tgggatgaac tttgagttta tgccagaact gaagtggagt
      661 ttcggctacc caggcgcgat tatctttatg atcctcgcgg gcctggcacc gtatctgtac
      721 tttaagcgca agaactggct gtaatatgag tgccggataa agctattttt ttatccggct
      781 tatttctaaa aattgtctta tttaaagtca tgcggattga aaactcgctc tattttattt
      841 tcttgtgtta attgatactc agtaaaatcg ttaataccaa tatgagtgtt acgataaggg
      901 tattcattaa taaccattaa gtaatttttc acgatgcaat tatcattgct cttaattgtc
      961 gggaaacagt agagatcttc atacggccct tcgatatatt ttttatagtc agtgaagcca
     1021 ttaccgatat caatgacata ccaggtatgc caactacctt ctggtagggg atgttgattt
     1081 ttaatttcat tctgatgctt cagataccaa tttattttat cgctgtcagt ccatggcaga
     1141 tgatcaacga ccagaaagac tgaattgcca gcatcagagt atgtatacaa aatctgtaca
     1201 ggacgcaatg tccataaatt atagatgcag catagaagaa ataaacataa tataattttc
     1261 tggagtttgc ttttcataga taattttcga ttctttcgat agtgttaaaa ttggtgaaaa
     1321 aaggtttgaa agcaaagtct ttatgccgtt gtaaaaagaa ccatatgcga aaaaatctat
     1381 atttgctgta agtcttgttt ttgatatcag ttacatcaag accgaagtgg tcctgtgcta
     1441 caaaatgaat tgttgcactc catcctatag cgtagttttg aaaacttaaa agagatattt
     1501 tttgagctga aatatcatga acactgattc ctaagccatt aaaactgtcc tcgaagtcat
     1561 taaatttatt tagtcttgat gaaagtaatt tggttttaat gctttgcagg ataagaggtt
     1621 ggtatcctgt cgagggagtt gaattaatgc cattttcgat acattctttg attattcgta
     1681 ttggactatt ataaccatat ttatttattt tttcatgaaa agataaattt aattgttggc
     1741 tatgaaaatt gctgccattt ccatatctaa aatgttcaat cagatcttcg ataagtgttt
     1801 tattttgtcc aaaaaaggaa aacatttttg ctagttcttt catttctgca aataaaatat
     1861 cagtgcattc acgttgtgat atttttgttc cggaacttga ggcactaaaa gcaacattaa
     1921 tagaacctgg gccagggaaa tggtatttaa taagtctgta aggatcaact tttgctgaga
     1981 tatcgcttag gccgagtgag agaaagtctt gttctaccat atcaccatat tgcatatcat
     2041 cagcagaaaa atcatcgaat aaactaatag tttcgaaaag tgtacaggga atattaattg
     2101 gcggaagtaa tatgttattc atttgcattt gccttggtta tatttaagct caatgtcaat
     2161 tcgagaacaa atgctagggt aaaaaaatat ttaaaaaaga agtacatttc acattttttc
     2221 attacatctc tgaagagaac ctcgttaatg attggtcttg ctcattgtta ataacaaaat
     2281 gttgaattta ccgattagga aactttttcc tacgcggatt gcctccagct atggcatgaa
     2341 ctttgagttt atgccagaac tgaagtggag ctttggctac cctggcgcga ttatctttat
     2401 gatcctcgcg ggcctgacac cgtatctgta ctttaagcgt aagaactggc tgtaactata
     2461 cgatgtcgct gtgagtaagt tagtgtgctg acagcgacat tttagccgag ggaatcgcag
     2521 ataacataat tgttgtgaat aatatccatg ccacgttgag ttggacgctt aatgactcac
     2581 atctcatccc ttcaattgct gcagagataa tagaaaaaag tgtcaagccc aatgaaatca
     2641 atacaaagta gattgcctta catccagtat ctcttattct ttttgctcca atattgatat
     2701 ttacataggt attattaata accagaataa aactcaaagc caggttgata tcattcaaag
     2761 ttattaatat tgaccatgat gttaatgtat agattatgaa ctgaaagcgt gataaacgct
     2821 caagcctgga tgggtagaat atatttctga tggatgattt gaacattttt acactgtttt
     2881 atatataatt gttttgaata tgtattacct gaattatgtg gtttgcatat atatatgcaa
     2941 tatagcagtc tttgcattga taactataca gtagtaattt agagtgtgta agtttttatt
     3001 gatggggtat attttaatta aatataattg aaatgtattt ttattctata aacaatatct
     3061 tgggtgaatg gattctatat gggaaatggt aacttttggc agttgttctt gggtaaagaa
     3121 ccttacagca aaaaatctga ttttgttaag gcatggttat tgtggtgggc tattaccatc
     3181 cttgttctat ttattttaag cctgactacg acattaaatt taagaagtat aggtataggg
     3241 ttaagttttg ggttttgtca aatccttggt agatatacga tgtctctagg atggagtaag
     3301 tactggggag ttttgggggt atttcctgta acaaatattg ttctttttct tatcttaatt
     3361 tttttcaaaa aaagataaat aagtcagtgg gtaggctctt taaacggcct acccatgtta
     3421 attcttaact ttttctacgc tgcgtataaa tcgcatccat cacaaaaatt gccagcgcca
     3481 cccaaataaa ggcgaaagtc accatcttat cggcacccgg tttttcacca taaaacgtca
     3541 cagccagcag gaacatcagc gtcgggccaa tgtactggaa aaagcctaac gttgagagac
     3601 gcaagcgcgt ggcagcggcg gtaaaacaca acagcggtac ggtagtgaca ataccggcgg
     3661 cgatcagcag taaattcagc gacatcgggt tttgccccat atggctggtt gagctgtcgg
     3721 caatagcaaa caggtaaatt gccgccacgg gcagcagcca catggtttcg attaacatgc
     3781 cggtttgcgc ttcaacggca atcttcttgc gtaccagacc gtagaaggca aaactaaatg
     3841 ccagtcccag cgcgataata ggtagcgaac caaaagtcca cagctggact aacacgccac
     3901 atatcgccag aatcaccgcc agccattgca tccggcggaa tcgctcgccg aggaaaatca
     3961 tccccagcac aatgttcacc agcgggttaa taaagtaacc aaggctcgct tccagcatat
     4021 ggtgattgtt caccgcccag ataaacagta gccagttgcc accaatcagc acggcagaga
     4081 ctgccagcat aaaaattttc tgtggcgtct gaatcagcgt ttttaaatag gaccactggc
     4141 ggcaaatgct catcagcacc accataaaga aaaacgacca gatcacgcga tgcgtcagga
     4201 tttcatcggc aggcacgtag taaatcaact tgaagtacgc tggcgctata ccccaaataa
     4261 aataagcggc aagagcgagt aatacgccct gccgcgtttg ttttgcatcc atcgggaata
     4321 ctcattttta attggtaaca gcagtttacc tgcttttatg tcttcaacct accatatagg
     4381 tggcggtggc actggcaata taaagctgtt cttcattgtg taattcaacg cgggcgacgg
     4441 cgactttatt gcctgcacgc aacaggctac tggtagcagt aaaacgctcg cccctgcctg
     4501 ggcgcagata atcaacgcga agatcaatgg tccccatccg cgatagccgc tggcgtagtt
     4561 catcttcact gatggtttcg tggcgggtta aggtacttcc cacgcacacc agaccggcgg
     4621 cgacatccag cgccgacgca atgaccccgc cgtgcaaaat gctttgcgcc cagttgccca
     4681 ccatcattgg ctgattttta aaggccagct gtgcgaactc tttttcgtaa cgctccagtt
     4741 ccatccccaa tgcgcggtta aatggcatgt gataaacaaa catctcaccc actaatttca
     4801 gggcttgttc agcggtcagt acggcagaca tatcatcctt acacttcatt agttaatgaa
     4861 atgttgattt tatgcttctt tgttgttggt ttctacttta ggaagggata actaacggct
     4921 atggagttaa gtatgtaaaa tagcccgcag aaaaatattc accttatcaa taattcgtta
     4981 cggagaacac gaccgatgcg gactctgcag ggctggttgt tgccggtgtt tatgttgcct
     5041 atggcagtat atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt
     5101 ggcagtatta tcgccaatat gctgcaggag catgacaatc cgttcacgct ctatccttat
     5161 gacaccaact acctcattta cacccaaacc agcgatctga ataaagaagc gattgccagt
     5221 tacgactggg cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag cctggcattt
     5281 ccgctgtggc gtgggatttt aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa
     5341 tcctggtggc aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa
     5401 ccgcaattgt tcctcggttt tgccaccgat taccgttttg caggttggac gctgcgcgat
     5461 gtggagatgg ggtataacca cgactctaac gggcgttccg acccgacctc ccgcagctgg
     5521 aaccgccttt atactcgcct gatggcagaa aacggtaact ggctggtaga agtgaagccg
     5581 tggtatgtgg tgggtaatac tgacgataac ccggatatca ccaaatatat gggttactac
     5641 cagcttaaaa tcggctatca cctcggtgat gcggtgctca gtgcgaaagg acagtacaac
     5701 tggaacaccg gctacggcgg cgcggagtta ggcttaagtt acccgatcac caaacatgtg
     5761 cgcctttata ctcaggttta cagcggctat ggcgaatcgc tcatcgacta taacttcaac
     5821 cagacccgtg tcggtgtggg ggttatgcta aacgatttgt tttgatgaac ggttgagtgg
     5881 ttggcaaatc tggaatccag catccaggat taccctctca gagactaaaa gcattgcagt
     5941 ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg
     6001 gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac aggttttaca agaaaccttt
     6061 ggctaccaac agtttcgccc cggccaggaa gaaattatcg acactgtgct ttccggtcgc
     6121 gattgcctgg tcgtcatgcc caccggtggc ggaaaatccc tttgctatca aattcctgcc
     6181 ttattgctaa acggccttac cgtggttgtt tcaccgctga tttcgttgat gaaagatcag
     6241 gtggatcaac tgcaagccaa cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc
     6301 gaacagcaac ttgaagtgat gacaggctgc cgcaccgggc aaattcgtct gctttatatc
     6361 gccccggaac gcctgatgct ggataacttt cttgagcatc tggcgcactg gaatccggtg
     6421 ttattagccg ttgatgaagc gcactgtatc tcccaatggg gccacgattt ccgcccggaa
     6481 tatgccgcgc tcggtcagtt gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc
     6541 gccacagccg acgacaccac gcgccaggat atcgtgcgcc tgctggggct gaacgatccg
     6601 ctgattcaaa tcaacagttt tgaccgtccg aatattcgct acatgctgat ggagaagttc
     6661 aaaccgctcg atcagttgat gcgctacgtg caggaacagc gcggtaagtc cggcattatc
     6721 tactgcaaca gccgggcgaa agtagaagac accgctgcgc gcctgcaaag caagggtatt
     6781 agcgcggcgg cctatcatgc cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa
     6841 ttccagcgcg atgacctgca aattgtggtg gcgacggtgg cgttcggcat gggcatcaat
     6901 aaaccaaacg ttcgcttcgt ggtccacttt gatattccgc gcaatatcga atcctattat
     6961 caggaaaccg gtcgcgccgg gcgtgatggc ctgcccgcgg aagcgatgct gttttacgat
     7021 ccggccgata tggcgtggct gcgccgttgt ctggaagaga agccgcaggg gcagttgcag
     7081 gatatcgaac gtcataaact caatgcgatg ggcgcttttg ccgaagcgca aacttgccgt
     7141 cgtctggtac tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat
     7201 atctgcctcg atccgccgaa acagtacgac ggttcaaccg atgctcagat tgccctttcc
     7261 accattggtc gtgtgaatca gcgttttggt atgggctatg tggtggaagt gattcgcggc
     7321 gctaataacc agcgtatccg cgactatggt catgacaaac taaaagtcta cggtatgggc
     7381 cgtgataaaa gtcatgaaca ttgggtgagc gtgatccgcc agttgattca cctcggcctg
     7441 gtgacgcaaa atattgccca gcattctgcc ctccaactga cagaggccgc gcgtccggtg
     7501 ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa
     7561 gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa attacgcaaa
     7621 ctgcgtaaat cgattgccga tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca
     7681 accttgattg agatggctga acagatgccg atcaccgcca gcgaaatgct cagcgttaac
     7741 ggtgttggga tgcgcaagct ggaacgcttt ggcaaaccgt ttatggcgct gattcgtgcg
     7801 catgttgatg gcgatgacga agagtagtca gcagcataaa aaagtgccag gatgaagact
     7861 ccgtaaacgt ttcccccgcg agtcaaatgt atgttgatgt tatttctcac cgtcgccatg
     7921 gtgcacattg tggcgcttat gagccccggt cccgatttct tttttgtctc tcagaccgct
     7981 gtcagtcgtt cccgtaaaga agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg
     8041 gtttgggctg ggattgcgtt gcttggcctg catttgatta tcgaaaaaat ggcctggctg
     8101 catacgctga ttatggttgg cggtggcctg tatctctgct ggatgggtta ccagatgctg
     8161 cgcggcgcgc tgaaaaaaga ggcagtttct gcacctgcgc cacaggtcga gctggcgaaa
     8221 agtgggcgca gtttcctgaa aggtttactg accaatctcg ctaatccgaa agcgattatc
     8281 tactttggct cggtgttctc attgtttgtc ggtgataacg ttggcactac cgcgcgctgg
     8341 ggcatttttg cgctgatcat tgtcgaaacg ctggcgtggt ttaccgtcgt tgccagcctg
     8401 tttgccctgc cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt
     8461 gccggggcgt tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg
     8521 cgtcttcaga gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat
     8581 gcctcgccga tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca
     8641 gcgccttcat ctgctttggt cctttaatcc atagagcaat ccgttgagca agggtggcgt
     8701 aaccgatcat cacaataata tcgaccacaa tagtggtgac gccgagcacg atatactgca
     8761 tcagttgcgg ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac
     8821 ttttgggatt ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag
     8881 tagaggccag cgatttaagg tcaattgcac cagcggcgcg ccactgctgg attcccagcc
     8941 aaatcaagta agccgcgcct gcccacttca acacttcaaa cgcaatcact gagcgggaaa
     9001 atagcgtccc caaccccacg ccaaccagca caatatgaat cgccagtccg gtttgaagcc
     9061 cagcaataga cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga
     9121 ttgcaccaga gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc
     9181 attctaaggt catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc
     9241 gcagcgttgt gtcaggcacg ctaaaaaaaa cgattttacg tggtttaaga ggcagattac
     9301 ccgatgtttc agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc
     9361 atgggaccgc tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat
     9421 gacattccgg tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc
     9481 tgcccggggc gtattgagag ctacgtaaaa tatgcggaac tggcctatga cctgttccat
     9541 ttggggtttg atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta
     9601 gccgatccgc atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca
     9661 ttctggcagc aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg
     9721 atgggcggtg cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt
     9781 gcgctaactg cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag
     9841 atcctcaact gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg
     9901 cgctggcgcg cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga
     9961 cgtaacttac gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat
    10021 tgggtacgcg aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc
    10081 acgccaacgc ttctcttaca agctgaagag gaacgcgtgg tggataaccg catgcatgac
    10141 cgtttttgtg aactccgcac cgccgcgggc catcctgtcg aaggaggacg accgttggta
    10201 attaaaggtg cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc
    10261 cacgccatcg ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag
    10321 gtttaaattt cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc
    10381 cgaccatacg ttatcccctt acgccaaaga aactctgaag ctgctcaccg cgcgcggcat
    10441 caactttgtg tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct
    10501 ggagattaag tcttacatga ttacctccaa tggtgcgcgc gttcacgatc tggatggtaa
    10561 tctgattttt gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa
    10621 cgacaatccg gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca
    10681 tcgcccggaa gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc
    10741 tggattactg gagccggaag gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca
    10801 actgctgccg ctggagcagg cgattaacgc tcgctggggc gatcgcgtca acgtcagttt
    10861 ctctacctta acctgtctgg aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga
    10921 agcagtggcg aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat
    10981 gaacgacgcc gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca
    11041 gcgtctgaaa gaccttcatc ccgagctgga agtgattggt actaacgccg aagacgcggt
    11101 gccgcattat ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa
    11161 cctgatactt cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgctact
    11221 tatcatcacc acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg
    11281 gcacgtcgat agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct
    11341 gccgtttctg cgtacccgtg gcaatagcct gaaaacagtc ggcctgtata tgctggtggg
    11401 cgcgatgcag ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt
    11461 ttccgagctg ctgctgttca ccgtgctgac gccgctctac atcacgctga tttatgacat
    11521 catgagtaag cgccgtctgc gctggggcta tgcttttagc gccttgctgg cggtgattgg
    11581 tgccgggatt attcgctatg atcaggtcac cgaccatttc tggactggct tgttgctggt
    11641 gcaactctcc aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac
    11701 tcgcccgatg ccgcagcata acgcctttgc gtggttctat cttggcgcgt tcctggtggc
    11761 ggtaattgca tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgctgcaatg
    11821 gggcattctg gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta
    11881 cggcgcgacg caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc
    11941 agggctgctg gtaaacctgg ctatctggca ccaacagccg cactggccaa cgtttattac
    12001 aggcgcgctg gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc
    12061 ttcacaaacg gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta
    12121 actggctgac gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg
    12181 cccagggttt tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt
    12241 agcgcggcaa tacccatccg cgcggcaacc atctgaatca acaataaggt gttatcgacg
    12301 cttttcagtg acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga
    12361 ctacgctgca ccggataaat caacagcgtc tcgctggcga gatcttccgg tgtaattcgc
    12421 gttttcgccg ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc
    12481 ggcgaataat gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg
    12541 ccctgttgca acgcgggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc
    12601 tgcggccagt tcttatggaa attttctaac gcgggcgtca gccactgaat acagctatgg
    12661 cactcaatgg caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg
    12721 ctaatttgcg gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag
    12781 cgtagcggct ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta
    12841 aactggtgag acagggcgga ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag
    12901 ccgcagttcc gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt
    12961 cacttcggca tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg
    13021 taaacatctg gacggctaaa tactaaaaat tccataaggg gcatataatg acaattctta
    13081 atcacaccct cggtttccct cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa
    13141 gttattgggc ggggaactcc acgcgtgaag aactgctgac agtagggcgt gaactgcgtg
    13201 ctcgtcactg ggatcaacaa aagcaagcgg gtatcgacct gctgccggtg ggcgattttg
    13261 cctggtacga tcatgttctg accaccagtc tgctgctggg taatgttccg ccacgtcatc
    13321 agaacaaaga cggttcggta gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc
    13381 cgactggcga acctgcggcg gcagcggaaa tgaccaaatg gtttaacacc aactatcact
    13441 acatggtgcc ggaattcgtt aaaggccaac agttcaaact gacctggacg cagctgctgg
    13501 aggaagtgga cgaggcgctg gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg
    13561 ttacttacct gtggctgggg aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga
    13621 acgacattct gccggtttat cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt
    13681 gggtacagat tgatgaaccc gcgctggtac tggaactacc acaggcgtgg ctggacgcat
    13741 acaaacccgc ttacgacgcg ctccagggac aggtgaaact gctgctgacc acctattttg
    13801 aaggcgtaac gccaaatctc gacacgatta ctgcgctgcc tgttcagggt ctgcatgttg
    13861 acctcgtaca tggtaaagat gacgttgctg aactgcacaa gcgcctgcct tctgactggt
    13921 tgctgtctgc gggtctgatc aatggtcgta acgtctggcg cgccgatctt accgagaaat
    13981 atgcgcaaat taaggacatt gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt
    14041 tgctgcacag ccccatcgac ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct
    14101 ggtttgcctt cgccctacaa aaatgccatg aactggcact gctgcgcgat gcgctgaaca
    14161 gtggtgacac ggcagctctg gcagagtgga gcgccccgat tcaggcacgt cgtcactcta
    14221 cccgcgtaca taatccggcg gtagaaaagc gtctggcggc gatcaccgcc caggacagcc
    14281 agcgtgcgaa tgtctatgaa gtgcgtgccg aagcccagcg tgcgcgtttt aaactgccag
    14341 cgtggccgac caccacgatt ggttctttcc cgcaaaccac ggaaattcgt accctgcgtc
    14401 tggatttcaa aaagggcaat ctcgacgcca ataactaccg cacgggcatt gcggaacata
    14461 tcaggcaggc cattgttgaa caggaacgtt tgggactgga tgtgctggta catggcgagg
    14521 ccgagcgtaa tgacatggtg gaatactttg gcgagcacct cgacgggttt gtctttacgc
    14581 aaaacggttg ggtacagagc tacggttccc gctgcgtgaa gccaccgatt gttattggtg
    14641 acgttagtcg cccggcaccg attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg
    14701 acaaaccggt gaaagggatg ctgacggggc cggtgaccat actctgctgg tcgttcccgc
    14761 gtgaagatgt cagccgtgaa accatcgcca aacagattgc gctggcgctg cgtgatgagg
    14821 tggccgatct ggaagcggca gggattggta tcatccagat tgacgaaccg gcgctgcgcg
    14881 aaggtttacc actgcgtcgt agcgactggg acgcctatct ccagtggggc gtagaggcct
    14941 tccgtatcaa cgccgccgtg gcgaaagatg acacacaaat ccacactcac atgtgttatt
    15001 gcgagttcaa cgacatcatg gattcgattg cggcgctgga cgcagacgtc atcaccatcg
    15061 aaacctcgcg ttccgacatg gagttgctgg agtcgtttga agagtttgat tatccaaatg
    15121 aaatcggtcc aggcgtctat gacattcact cgccaaacgt accgagcgtg gaatggattg
    15181 aagccctgct gaagaaagcg gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg
    15241 actgtggcct gaaaacgcgc ggctggccag aaacccgcgc agcactggcg aatatggtgc
    15301 aggcggcgca gaacttgcgt cgggggtaaa tccaaaccgg gtggtaatac cacccggtct
    15361 tttctcatta cagcgacttc ttcccaccat actgcttaaa ccattccagc atacgctgcc
    15421 agccatcttt cgcagattcg gcatgatagc tcgggcgata atcagcgttg aatgcatgcc
    15481 cggcatccgg gtacacgata atctctgctt tcgcattagc agcccgcagc gcctggcgca
    15541 tggtttcaac gctctcctgc ggaatgctgt tatcctgacc gccatataag ccgagaaccg
    15601 gcgcgttaag atcggttgcg atatcaacag gttgtttcgg tgaattcagc gacttgtcac
    15661 ccgtcagttt gccgtaccac gccactgcgg cttttagctg tggattatgc gcggcataca
    15721 gccaggtgat acgtccaccc cagcagaatc cggtgatcat taaacgatga acatcgccgc
    15781 cgttgcggga agcccaactg gcgacatgat cgagatcggc cagcacctgc gagtcaggca
    15841 cttttgctac cagaccgcta agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt
    15901 cgcggaagta aagttcaggt gcgatagcca gatacccctc cagcgccaga cggcgacaaa
    15961 tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac tacaatgacc actggcagtg
    16021 ggccatcgct ttgctttggt ctggcatggt aagcaggcat gttatcccct tgtgaaggga
    16081 tagaggtgaa gcccgccaca atcgcgtcgt ccggggtctg aacgatggta gaagcgagag
    16141 gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc catggtattc tccgtaccct
    16201 tataaaaatg ttgcgcaatg ttaactatag tcagcatgca acaaatcaca ttgcctgaat
    16261 cggctcatct tttatgcagt cctgcagaat gaagagtgat ttatgtgatt tacatcactt
    16321 ttgatgggta aatttatgca acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt
    16381 taaacccttc ggtaaagtgt ctttttgctt cttctgacta aaccgattca cagaggagtt
    16441 gtatatgtcc aagtctgatg tttttcatct cggcctcact aaaaacgatt tacaaggggc
    16501 tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga
    16561 taagccggtt aagctggcat ctcaccgcga attcactacc tggcgtgcag agctggatgg
    16621 taaacctgtt atcgtctgct ctaccggtat cggcggcccg tctacctcta ttgctgttga
    16681 agagctggca cagctgggca ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca
    16741 gccgcatatt aatgtgggtg atgtcctggt taccacggcg tctgtccgtc tggatggcgc
    16801 gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct gatttcgaat gtacgactgc
    16861 gctggttgaa gctgcgaaat ctattggcgc gacaactcac gttggcgtga cagcttcttc
    16921 tgataccttc tacccaggcc aggaacgtta cgatacttac tctggtcgcg tagttcgtca
    16981 ctttaaaggt tctatggaag agtggcaggc gatgggcgta atgaactatg aaatggaatc
    17041 tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt
    17101 tatcgttaac cgcacccagc aagagatccc gaatgctgag acgatgaaac aaactgaaag
    17161 ccaagcggtg aaaatcgtgg tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt
    17221 ctgaaggccg acgcgttcgg ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt
    17281 tccaactgga cgtttgtaca gcacaattct attttgtgcg ggtaagttgt tgcgtcagga
    17341 ggcgttgtgg atttctcaat catggtttac gcagttattg cgttggtggg tgtggcaatt
    17401 ggctggctgt ttgccagtta tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt
    17461 gaagagatgg tcgcggagtt aagcgcggca aaacaacaaa ttacccaaag cgagcactgg
    17521 cgtgcagagt gcgagttact caataacgaa gtgcgcagcc tgcaaagtat taacacctct
    17581 ctggaggccg atctgcgtga agtaaccacg cggatggaag ccgcacagca acatgctgac
    17641 gataaaattc gccagatgat taacagcgag cagcgcctca gtgagcagtt tgaaaacctc
    17701 gccaaccgta tttttgagca cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg
    17761 aacagcctgt tgtcgccgct acgtgaacaa ctggacggtt tccgccgtca ggttcaggac
    17821 agcttcggta aagaagcaca agaacgccat accctgaccc acgaaattcg caatctccag
    17881 caactcaacg cgcaaatggc ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac
    17941 aataaaaccc agggcaactg gggcgaggta gtattgacgc gggtgctgga ggcttccggt
    18001 ctgcgtgaag ggtatgaata tgaaacccag gtcagcatcg aaaa
//
LOCUS       DAFATA010000091        17988 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFATA010000091 DAFATA010000000
VERSION     DAFATA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.092"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..144
                     /locus_tag="LGJ20_003638"
     CDS             <1..144
                     /locus_tag="LGJ20_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK2387652.1"
                     /translation="NIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGG
                     WAI"
     gene            295..1944
                     /locus_tag="LGJ20_003639"
     CDS             295..1944
                     /locus_tag="LGJ20_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBK2387653.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2098..3390)
                     /locus_tag="LGJ20_003640"
     CDS             complement(2098..3390)
                     /locus_tag="LGJ20_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK2387654.1"
                     /translation="MRYNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKHFIDIALYANNK
                     CDDRITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDDCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     RQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(3568..5217)
                     /gene="actP"
                     /locus_tag="LGJ20_003641"
     CDS             complement(3568..5217)
                     /gene="actP"
                     /locus_tag="LGJ20_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBK2387655.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(5214..5528)
                     /locus_tag="LGJ20_003642"
     CDS             complement(5214..5528)
                     /locus_tag="LGJ20_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBK2387656.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(5728..7686)
                     /gene="acs"
                     /locus_tag="LGJ20_003643"
     CDS             complement(5728..7686)
                     /gene="acs"
                     /locus_tag="LGJ20_003643"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBK2387657.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            8078..9514
                     /gene="nrfA"
                     /locus_tag="LGJ20_003644"
     CDS             8078..9514
                     /gene="nrfA"
                     /locus_tag="LGJ20_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBK2387658.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            9559..10125
                     /gene="nrfB"
                     /locus_tag="LGJ20_003645"
     CDS             9559..10125
                     /gene="nrfB"
                     /locus_tag="LGJ20_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBK2387659.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            10122..10793
                     /gene="nrfC"
                     /locus_tag="LGJ20_003646"
     CDS             10122..10793
                     /gene="nrfC"
                     /locus_tag="LGJ20_003646"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBK2387660.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            10790..11746
                     /gene="nrfD"
                     /locus_tag="LGJ20_003647"
     CDS             10790..11746
                     /gene="nrfD"
                     /locus_tag="LGJ20_003647"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBK2387661.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            11766..13484
                     /locus_tag="LGJ20_003648"
     CDS             11766..13484
                     /locus_tag="LGJ20_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBK2387662.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            13477..13860
                     /gene="nrfF"
                     /locus_tag="LGJ20_003649"
     CDS             13477..13860
                     /gene="nrfF"
                     /locus_tag="LGJ20_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBK2387663.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            13857..14453
                     /gene="nrfG"
                     /locus_tag="LGJ20_003650"
     CDS             13857..14453
                     /gene="nrfG"
                     /locus_tag="LGJ20_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBK2387664.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            14795..16108
                     /gene="gltP"
                     /locus_tag="LGJ20_003651"
     CDS             14795..16108
                     /gene="gltP"
                     /locus_tag="LGJ20_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBK2387665.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(16186..16875)
                     /locus_tag="LGJ20_003652"
     CDS             complement(16186..16875)
                     /locus_tag="LGJ20_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBK2387666.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(16969..>17988)
                     /locus_tag="LGJ20_003653"
     CDS             complement(16969..>17988)
                     /locus_tag="LGJ20_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBK2387667.1"
                     /translation="AHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFE
                     DLELVIVQDIFMTKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTD
                     WQIISEIATRMGYPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDA
                     DQGTSYLFKEKFDTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGN
                     CAALAALADEPGYAQINTEDAKRLGIEDEALVWVHSRKGKIITRAQVSDRPNKGAIYM
                     TYQWWIGACNELVTENLSPITKTPEYKYCAVRVEPIADQRAAEQYVIDEYNKLKTRLR
                     EAALA"
BASE COUNT         4082 a         4553 c         4838 g         4515 t
ORIGIN      
        1 aacatcgtaa caggcatcat gatcggcttc gcgactctgg tgattggtcg tctggtttcc
       61 ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct ggtgaccttc
      121 tatgcgggtg gctgggctat ctaatctttc atcggattct gaaaacgggt ggcaatggct
      181 gcccgttttt attttctccg cacattgtgt gagcttttgc gatattctga aaaaatgaga
      241 attcaggcat aacgtctttc tcagatcata aaaaacatcg caaacaggga acgcatggaa
      301 atcttcttca ccatactgat aatgaccctc gtggtctcgc tgtccggggt ggtcactcgt
      361 gtcatgccct ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc gctactggcg
      421 tggccgacgt ttggtttgca tgtggagttt gatcctgaac tctttttagt cttgtttatc
      481 ccgccgttgc tgttcgctga tggctggaaa acgccgaccc gtgaatttct cgaacatggt
      541 cgagagattt ttggcctcgc gctggcgctg gtggtggtca ccgtggtcgg cattggcttc
      601 cttatttact gggtggtgcc gggcattccg ctgatccccg cctttgcgct ggcggcggta
      661 ctttctccga ccgatgctgt ggcgctctcc gggattgttg gcgaagggcg tatcccgaaa
      721 aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg acgcttccgg cctggtgtcg
      781 ttgaagtttg ccgtagcagt ggcgatgggg acgatgatct tcaccgtcgg cggtgcgacg
      841 gtcgaattta tgaaagtagc cattggcggt attctcgccg gttttgtggt tagctggctg
      901 tacggtcgct cgctgcgatt cctcagccgc tggggcggtg atgaacccgc gacgcagatc
      961 gtcctgctgt tcctgctgcc attcgcttct tatctgattg ccgaacatat tggcgtttcc
     1021 ggcatcctcg ctgcggttgc cgccgggatg accatcaccc gctccggtgt gatgcgccgt
     1081 gcgccgctgg caatgcgcct gcgtgcaaac agcacctggg cgatgctgga atttgtcttt
     1141 aacggcatgg tgttcctgct gttaggtctg cagctaccgg gtattctgga gacgtcgctg
     1201 atggcggcag aaatcgaccc taacgtcgaa atctggatgc tgtttaccga tattattctg
     1261 atatatgcgg cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa aaagttcagc
     1321 aaccgcttcc tgaagaagaa gccgatggag tttggttcgt ggaccacacg agaaatcctg
     1381 atcgcgtctt tcgctggggt gcgtggggcg atcactctgg ccggtgtgct ctctatcccg
     1441 ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt cctggcggct
     1501 ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt tgcctattct gctacaacac
     1561 attgaagtcg cggatcattc gcaacaattg aaagaggaac gtattgcgcg agcggcaacg
     1621 gcagaagtgg cgattgtggc gatccagaaa atggaggagc gtctggcggc ggataccgaa
     1681 gagaatatcg ataaccagct gcttacggag gtcagttctc gcgtcattgg taacctgcgt
     1741 cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc aggaagagaa ccttgagcgt
     1801 cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac tgtaccacct gcgcgccacg
     1861 cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt gcttgaagcg
     1921 ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga ggtaatctga
     1981 aaaaggtgca atcttttgca cctttaacag gttagctcgg gctaacattc atgattctaa
     2041 aacaaataag ttatctctct tgtaaagtca attcattggc tgttcttcga tttaacctca
     2101 atctgcgtgt tgcataatat ctgcagtaat cttcatagcg actgcattaa tattatcctc
     2161 agtttgtgac atgccagtat gagaggtaca atcttcttca aagaatttct gtcgtgttac
     2221 cagggtatca ctatcaatgg ggatattttc ctccccacgg agtatggaaa tgcccattcc
     2281 ctggacgcta gcctcatcaa aattaacccg atagagacgg gtattaaaca tacaggcttt
     2341 tgttaaaata gaacagttga aatggcagtt gcttaaattg gcattattaa aattaacatt
     2401 agccatatta gagtcggtac atataaccat ttcgaggaat gtatttgtta gccacgtaca
     2461 aaagagtttg ctatcaatta agtttatatt ctggaggatt gctttgttca gattgacgcg
     2521 agacagatca gcatgttcaa agttaattaa atcgagttta gagaaactaa ggtcggcttt
     2581 aaataaatta acatatggcg ctattacttt ataaagattt acctcagcca tgtacgccat
     2641 ataaaaattt gaatagtcaa gaaaggtatt tttaatattg gctctattca ttgctgcatt
     2701 gtagaattga acatgcatag cggagcatgc atttagagtt ccgtcggcca tttttatctg
     2761 agcgaaattt gagtcatcga gaatacaatc atcaaaaagt attttttcca ttatggcgta
     2821 ataaaaattc gttttataca tattacagtc ctggaaaata gcgttttgta gtctgcaacc
     2881 tgcaaaattc gaattggata aatcttcatc tttaatcacg atacctttca ggctaaggtg
     2941 tgaaaaatcg taaccaccat ttactgtctg tggaatttcg atagtattat tatggacggc
     3001 gacattattt ataaaatata ccaccttaat ggatacttcc ttttctttac taaggtaaac
     3061 tcttctcgta attctatcgt cacatttatt atttgcatat aaagctatat ctataaaatg
     3121 tttatgtatt ttcctgtccg tttccttcgt gatttttcct gatggtaaac tatcataaat
     3181 tcttttcatt ttctgaatca aacctatcac tttttcatga tcatccatct tgaaaagaga
     3241 gagggcttta ttgtcaatta acgtgttttt atgatgtttg ctgtaattat atgattttgt
     3301 tttctttgta tgtgatgaac ttgtgaaaga gtggttatcg ttaatctggc aaagagagaa
     3361 aaacatatta tttaaaccat tatagcgcat aaaatatcct tataattaac aagaaagaaa
     3421 aggcatattc tctgcattat cattttctac gactgtcaaa aatcgctcat tttttaatga
     3481 gtttatttgt ttaatattat gggaaaaggt gatgcatgct ggagaggaag agtattcccc
     3541 ggtcaggcga ccggggaagg ggagagatta atgtgcgcgg ccttgctcaa cgccaaagcc
     3601 ggtctgggag cggataaact gtgcacggaa cagttcacgc tcgcgcgcac cttccgctga
     3661 gttatcggtt gccgagaaga accagatgcc gaggaatgcc acgctgatag agaacagcgc
     3721 cgggtattca tacgggaaga tggctttttc gtgaccaagg atctgtaccc aaatcgtcgg
     3781 gccgaggatc atcagtacca ctgcggtaat cagccccagc cagccaccca tcatcgcgcc
     3841 acgagtggtc agtttcgacc agtacataga aagcagaatg atcgggaagt tacagctcgc
     3901 tgcgatggca aacgccaggc ctaccatgaa ggcgatgttc tgattctcaa acagcacgcc
     3961 gaggataatg gcaatcacac ctaaaatcag cacggtgatt ttcgataccc gcagttcttc
     4021 acgttcggtc gcgccttttt tgaagacgtt agcgtacagg tcatgcgaaa ccgctgatgc
     4081 gcccgccagc gtcagaccag caaccaccgc aagaatggtg gcaaaagcaa ccgctgaaat
     4141 aaaaccgagg aacaggttgc cgcccactgc attcgccagg tgaacggccg ccatgttgtt
     4201 accaccaatc agatggcccg ccgcgtcttt atattccgga ttcgcaccaa ccagcatgat
     4261 cgcgccgaag ccgataataa aggtcagaat atagaagtag cccataaacc cggtggcgta
     4321 gaacacgctc ttacgtgctt cgcgggcatc gctgactgta aagaagcgca tcagaatgtg
     4381 cggcaagccc gccgtaccaa acatcagtcc cagacccaga gagagcgcgg agatcggatc
     4441 tttcaccagc ccgcccggct tcatgatgtc gacacctttc gggtgtaccg ccatcgcttc
     4501 actgaacaga ttgttgaagc taaagccgac gtgtttcatc accataaagg ccataaagct
     4561 ggcaccgaac agcaacagca cggctttgat aatctgtacc caagtggtcg ccagcatgcc
     4621 gccgaacagg acgtacatca tcatcagcac gccgaccagc accaccgcaa tgtgatagtt
     4681 aaggccaaac agcagctcga tcagtttacc tgcgcccacc atctgggcga taaggtaaag
     4741 cgccaccacc accagagaac cacaggccga aagaatacga atcggccctt gtttcagacg
     4801 gtaagaggcc acatcggcaa aggtgtagcg ccccaggtta cgcagacgtt cggcaatcag
     4861 gaacaaaatg atcggccagc ccaccaggaa gcccagcgag taaattaagc cgtcatagcc
     4921 ggaggtaaac accagcgcgg agatccccaa gaatgaggcg gcggacatat agtccccggc
     4981 aatcgccagc ccgttctgga agccagtgat attgccgcct gcggtgtagt agtcgctacg
     5041 agaacgtacg cgttttgatg cccagtaggt aatgccgagc gtaaacacga cgaaaatcag
     5101 gaacataata atcgcctgcc agttcgttgg ctggcgctct acggccccgc taatagcatc
     5161 cgcggcgtta gctgcgaaag ggagtgtggc ggcaagcgcc gtcagaactc ttttcatgat
     5221 gcttgtacct catgcaggac ttcattatta agacggtcga attcgccgtt cgcccgccag
     5281 atgtagatac cggtgagaac aaaggagatc acaatcactc caacaccaat tggaataccg
     5341 cgtgtgacgc tggtgttcgg attcagcggc gtgcccagcc agccgggcgc gaaggcgatc
     5401 agtaaaataa agccgatata aactgccagc ataataatcg acaggatggt ggcaaaccgt
     5461 tgccgttttt cgactaactc cctgaaatgc gcattgtctt ctatccgctg ataaatagtg
     5521 ccattcatca cagattctcc agaggtaatg tagggattgt tttaattccc gctcccttat
     5581 gggagaaggt taacgctcgg gtaacccttg ccgaatgtag gccggataag gcgtttacgc
     5641 cgcatccggc aatcaatgcc tgatgcgacg ctgtcgcgtc ttatcaggcc tacaaaccgt
     5701 taccgactcg catccggcaa ttgtgggtta cgatggcatc gcgatagcct gcttctcttc
     5761 aagcagcttc tcgactacgc caggatcggc aagcgtcgag gtatcgccca ggttgctggt
     5821 atcgcccgcc gcaattttgc gcagaatacg gcgcataatt ttgccggagc gggttttagg
     5881 cagggagtcg gtccagtgca gcacgtctgg cgtcgccagc gggccaatct ctttacgcac
     5941 ccagttgcgg acttctgcgt acagttctgg tgacggttcc tccccgtgat taagcgtgac
     6001 gtaggcgtag atcgcctgac ctttaatatt gtgcggaata cctactacgg cggcttcggc
     6061 aatcttcgga tgcgccacca gcgccgactc aatctctgcc gtccccagac ggtgaccgga
     6121 gacgttcagc acgtcgtcca cacgcccggt gatccagtaa tagccatctt catcgcgacg
     6181 cgcgccgtcg ccgctgaaat acatattttt gaaggtggag aagtaggtct gttcaaaacg
     6241 ttcgtgatcg ccaaacagcg tacgcgcctg acccggccag gagtcggtga tcaccaggct
     6301 gccttcggta gcgccttcca gcgggttacc ttcgttatcg accagcgccg gttgcacgcc
     6361 gaagaacgga cgtgttgccg aaccggcttt cagctcggta gcgccaggca gcggcgtgat
     6421 catgaaaccg ccagtttcgg tctgccacca ggtatcgacc accggacatt tctcgttgcc
     6481 gatttttttc cagtaccact cccacgcttc cgggttaatt ggctcgccca cggaaccgag
     6541 aatgcgcagc gacgaacggt cggtaccctc gatcgcttta tcgccttccg ccatcagcgc
     6601 gcggatcgcc gtgggtgcgg tatagagaat attgacctga tgcttgtcca ccacctgcgc
     6661 catacgggca ggcgtcggcc agttgggtac gccttcaaac atcagcgtgg tcgcaccgca
     6721 ggccagcggg ccgtacagca agtaactgtg tccggtcacc cagcccacat cggcggtgca
     6781 ccagtagata tcacccggat gataatcaaa gacatattta aaggtcagcg ccgcgtacac
     6841 cagataaccg ccggtagtat gcagcacacc ttttggctta ccggtagaac cggaggtgta
     6901 gagaataaac agcggatctt cggcgttcat ctcttccgcc tggtgctgat cgctcgcttg
     6961 ctcaaccagg tcgtgccacc acaggtcgcg cccttcctgc cagtcaattt tcccgccagt
     7021 acgcttcagt accaccacat gctctacgct ggtgacgttc gggtttttca gcgcgtcatc
     7081 aacgtttttc ttcagcggaa tactgcgccc ggcacgcaca ccttcgtcgg aagtgatcac
     7141 cagtcgtgag ttggaatcaa taatgcgccc ggcaacggct tccggcgaga agccgccgaa
     7201 aatcaccgaa tgcaccgcgc caatgcgggc gcaggccagc atcgcaaccg cggcttccgg
     7261 caccatcggc atataaatcg ccaccacatc acctttttta atgcccagct cgagcagggt
     7321 attggcgaag cggcagacgt cgcggtgcag ctctttatag ctgatatgtt tgctctggct
     7381 ggcgtcgtcg ccttcccaga tgatggcggt gcgatcgccg ttttcttgca gatggcggtc
     7441 aaggcagttt gccgccagat tcagcgtgcc gtcctcgtac catttaatgg acacattacc
     7501 gggggcaaag gaggtatttt tcaccttctg gtaaggtttg atccagtcga gaatttttcc
     7561 ctgttcgccc cagaaggtat caggtacgtt aatagattgt tgatacatcg cctcgtactg
     7621 ctgagggttt atcaggcaac ggtctgcgat gttggcagga atggtgtgtt tgtgaatttg
     7681 gctcatgctt ttgttctcct tgtaggatgt taataatatg tggcataagc gttaaatgta
     7741 ggggtattgg cagttttgtt tagtatttgg gcgacagatc acgcaaaagt agaattgtgc
     7801 aaataaacgg cagggtaatt tttgaaggtc agcaacaaaa gttgattaat tctttgagga
     7861 acatgcagtt atgcatgctg ttgaaaaaga ggaagatact gactaactct aaagtggtat
     7921 tttacatgca cttacaattg attaaagaca acattcacag tgtggttatt tgttacacat
     7981 aggggcgagc aatgtcatga cagtgtaggt gcggttactg tcgtgaaaac aataaaaacc
     8041 gccattgcaa caatggcgca attcggatga agcccctatg acaaggataa aaataaacgc
     8101 acgccgtatc ttcagcttat tgattccttt tttctttttc acttctgttc acgctgaaca
     8161 aacggcagct cccgcaaaac ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca
     8221 gcatcccgat caatatctct cctggaaagc cacctcggaa cagtcagagc gtgttgacgc
     8281 cctggcggaa gatccacggc tggtgatcct gtgggcgggg tatcccttct cgcgcgatta
     8341 caacaagccg cgtggacatg cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg
     8401 tgcgccgaaa aacgctgaag atggtccgct accgatggcg tgctggagtt gtaaaagccc
     8461 ggatgtggcg cgtctgatcc agaaagacgg cgaagatggc tacttccacg gtaagtgggc
     8521 gcgcggcggc ccggaaatcg tcaacaactt aggttgtgcc gactgccata acaccgcctc
     8581 accagagttc gccaaaggca aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc
     8641 gatggaagcc attggtaaac cttttgagaa agccggacgt ttcgaccagc aatcgatggt
     8701 ttgcggtcag tgccatgtgg agtattactt cgacggcaaa aacaaagcgg ttaaattccc
     8761 gtgggatgac ggcatgaaag tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc
     8821 tgactggact aactccctgt cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga
     8881 aacctggaca gcgggcattc acggtaaaaa caacgtgacc tgtatcgact gccatatgcc
     8941 aaaagtgcag aacgccgaag gcaaactcta caccgaccat aaaattggta atccgtttga
     9001 taacttcgcc cagacttgtg cgaactgcca tacccaggac aaagctgcct tgcaaaaagt
     9061 ggtcgcggaa cgtaagcagt cgattaacga cctgaaaatc aaggttgaag atcaactggt
     9121 tcacgctcac ttcgaagcga aagcagcgct ggatgcaggc gcgacggaag ccgaaatgaa
     9181 gccaattcag gacgatatcc gtcatgccca gtggcgctgg gatctggcga tcgcttccca
     9241 cggcattcat atgcacgcac cggaagaagg tttacggatg ctcggtacgg cgatggataa
     9301 agcggcggat gcacgcacca aactggcgcg cctgctggcg accaaaggca tcacccatga
     9361 aatccagatc ccggatatct caaccaaaga gaaagcccag caggctattg gcctgaacat
     9421 ggaacaaatc aaggccgaga agcaggactt catcaaaacg gtgatcccgc agtgggaaga
     9481 acaggcacgt aaaaacggtc tgttaagcca ataaccccgt tccgcctcgc aaggggcgga
     9541 aaacacaatg gagtgaatat gagcgtatta cgttcgttat taactgccgg ggtgctggcg
     9601 tcgggcctgt tgtggagcct gaacggaatt accgctaccc ctgcggcgca ggcatctgat
     9661 gatcgctacg aagttaccca gcagcgtaac ccggatgccg cctgtctgga ctgtcataag
     9721 ccagataccg aaggtatgca tggaaaacat gcttccgtta tcaacccgaa taacaaactg
     9781 ccggtcacct gcaccaactg tcacggccag ccatcaccgc aacaccgcga aggggtgaaa
     9841 gatgtgatgc gctttaacga gccgatgtac aaggttggag agcagaacag cgtctgtatg
     9901 tcctgtcatc tgccggaaca gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc
     9961 aaagtggcgt gcgccagctg ccattccctg catccacagc aagatacgat gcagacgtta
    10021 agtgacaaag gacggattaa gatttgcgtc gattgccaca gcgatcagcg caccaatccg
    10081 aactttaacc cggcgtctgt tccgttgctt aaggagcagc catgacctgg tctcgtcgcc
    10141 agtttctcac cggcgtcggc gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg
    10201 cgaaaacgtt gaatatcaat ggggtgcgtt acggcatggt gcatgacgag tcgttatgca
    10261 tcggctgtac cgcctgtatg gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct
    10321 cgcgcctgac gattattcgt agcgagccgc agggcgaatt tcctgatgtg aaatatcgct
    10381 tcttccgtaa gtcttgccag cactgcgatc atgcgccgtg cgttgacgtc tgcccgaccg
    10441 gtgcgtcttt tcgtgatgcc gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg
    10501 gttgtcagta ctgcatcgcc gcctgtccgt accgcgtgcg ctttatccat ccggtcacga
    10561 agacggcgga caaatgcgat ttctgccgta aaaccaattt gcaggccggt aagctgcccg
    10621 cgtgcgttga ggcttgcccg acgaaggcgc tgacgtttgg caatctggac gatcctaaca
    10681 gtgagatttc gcaactgctg cgccagaagc ctacttaccg ctacaagctg gcgctgggaa
    10741 ccaaaccgaa gctgtaccgc gtaccgttta aatacgggga ggtgagccaa tgacgcagac
    10801 ttccgcattt cattttgaat cgctggtgtg ggactggccg attgccatct acctgttttt
    10861 gattggtatt tctgccggtc tggtgacgct ggccgtgctg ttacgtcgct tctacccgca
    10921 ggcgggcggt gcagacagta cgttgctgcg caccacgctg attgtcgggc cgggcgcggt
    10981 gatcctcggt ctgttgatcc tcgtcttcca cctgacaaga ccgtggacct tctggaagct
    11041 gatgttccac tacagtttta cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta
    11101 catggtggtg ctggtgctgt ggctggcgaa aatctttgaa catgatttgc ttgccctgca
    11161 acaacgctgg ttgccgaagc tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt
    11221 tcatcgcgga ctggaaacat tgatgctggt gttggcggtg ttgttggggg cttataccgg
    11281 ctttctgctg tcggcgctga aatcgtatcc gttcctcaat aacccgatcc tgccggtgct
    11341 gttcctcttc tccggcatct cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg
    11401 ccaacgcagt aacccgcatt ccacggaagc gcagtttgta caccgtatgg aaatccccgt
    11461 ggtatggggt gaaatcttcc tgctggtggc gttttttgtc ggtctggcgc tgggcgatga
    11521 cggtaaagtg cgtgcgctgg tggcggcatt aggtggcggt ttctggacgt ggtggttctg
    11581 gcttggtgtc gccgggctgg ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg
    11641 cagttccggc attcctgccg tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat
    11701 gctgcgcttt ttcattctct acgccgggca gttaacggtg gcgtaagcca gaaaagaggt
    11761 ggtttctgga cgtattcctt cctgaagtcg gttttctggc gttgttgtta agtctcgggg
    11821 tcaacgtgtt gaccccgttg acggcctttg cgggagtgcg gttgcgctgg cctgccatga
    11881 tgcgactcac ttgcatcggc attctggcgc agttcgcgct cctgttgctc gcctttggcg
    11941 tactgacgta ttgttttctc atcagcgatt tctcggtcat ttatgtcgcc caacatagct
    12001 acagcctgct gtcgtgggaa ctcaagctgg cagcggtgtg gggcggccat gaaggttcgc
    12061 tgctgctttg ggtgctgctg ctttccgcct ggagcgcgct gtttgcctgg cattatcggc
    12121 agcaaaccga tccgctattt ccgctgacgc tagccgtttt atctctcatg ctcgccgcac
    12181 tgctactgtt tgtagtgctg tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg
    12241 aaggccgcga tctcaatccg atgctgcaac atcccggtct tatctttcat ccaccgctgc
    12301 tttaccttgg ctatggcggt ttgatggtgg cggcgagcgt ggcgctggcg agtttactgc
    12361 gcggcgagtt tgatggtgcc tgcgccagaa tttgttggcg ctgggcacta cctggctgga
    12421 gcgcattaac ggcggggatc atcctcggtt cctggtgggc ctattgcgaa ctgggctggg
    12481 gcggctggtg gttctgggat ccggtggaaa acgcctcttt attaccctgg ctttctgcca
    12541 ctgcgctgct gcacagtttg tccctgacac gccagcggga gattttccgc cactggtcgc
    12601 tgttgctggc gatagtaact ctgatgctgt cgctactggg taccttaatt gtccgttctg
    12661 gcattctggt ttcggttcat gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca
    12721 gcctgtttgc actgattagc cttgcgtctc tggctctgta tggctggcga gcgcgggacg
    12781 gtggcccggt ggtgcgtttt tcggggttat cgcgggaaat gttaatcctc gctacgctgt
    12841 tgctgttttg cgcagtgcta ctgatcgtgc tggtgggaac gctttatccg atgatttacg
    12901 gtctgctggg ctggggacgc ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac
    12961 cgtttggcct gttgatgctg gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg
    13021 tgcagcttcc ggcgctggta gctcatgcgg gcgtgctgtt atttgccgct gggatcgtgg
    13081 tttccagcgt cagccgtcag gagatcagcc tgaatttaca gccgggtcag caggtgacgc
    13141 tggcaggata caccttccgt tttgagcgcc tcgatctgca agccaaaggc aattacacca
    13201 gcgaaaaagc gatagtggca ctgtttgacc atcagcaacg cattggtgaa ctgatgccgg
    13261 agcggcgttt ttacgaagca cgtcgtcagc aaatgatgga accgtcaatt cgctggaacg
    13321 gcatccatga ctggtatgcg gtcatgggtg aaaaaaccgg agcggatcgt tacgcttttc
    13381 gcttgtatgt acaaagcggt gtgcgctgga tctggggggg aggattgttg atgattgcgg
    13441 gcgcattgtt aagcggatgg cgggggagga agcgcgatga ataaaggact tctcacgcta
    13501 ctgctgttat ttacctgttt tgcgcgcgct caggtcgtag acacctggca attcgccaat
    13561 ccgcaacaac agcaacaggc gttaaatatt gccagccagt tacgttgtcc gcagtgccag
    13621 aatcaaaact tactggaatc caacgcgccg gtggcagtca gtatgcgcca tcaggtttac
    13681 agcatggtgg cggaggggaa gaacgaagtc gaaatcattg gctggatgac cgaacgctac
    13741 ggagattttg ttcgctataa cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg
    13801 ccagtggtgt tgttactgct gatggcactg atcctctggc gagtgagggc gaagcgatga
    13861 aagagcccaa aataccggtg aaaatgctga caacactcac gattttgatg gtatttctct
    13921 gtgtcggcag ttatctgtta agtccaaaat ggcaggcggt acatgcggaa tatcagcgtc
    13981 agcgcgatcc gctacatcag tttgccagcc agcaaacccc ggaagcgcag cttcaggcat
    14041 tgcaggataa aatccgcgct aatccacaaa acagcgaaca atgggcgtta ctgggcgagt
    14101 actatctgtg gcaaaacgat tacagcaatt cgctgctggc gtaccgtcag gcgttgcaac
    14161 tacgtggtga gaacgctgaa ctgtatgcgg cgctggcgac ggtgctttat taccaggcca
    14221 gccagcatat gaccgcccag actcgcgcaa tgatcgacaa agccctcgcg ctggactcta
    14281 acgaaatcac cgctctgatg ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc
    14341 aagccatcga actatggcaa aaagtgatgg atctcaactc accccggatc aaccggacac
    14401 agctggtcga gtcgattaat atggcgaaat tgttgcagcg gagatcagat taatcatctg
    14461 gtgatgaaga ttatgttatt gagttgtata aaaaataatc gaatgaacat atgccaaaaa
    14521 taatcaccaa tcagtattat tgcagattaa acaaataaaa atctttccat aacaaatggt
    14581 tattcattaa tcctgccata aactcgcgtt attatgcatt aatgcagcga aaagctctgt
    14641 tgttaaaggg ttgcgcaaca taccgcgcaa atgatactga tcataagcgt taaaaaaatc
    14701 tacaaaccaa cgcaacacaa ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa
    14761 cggggaacgg cgctccattg aggaagttat tctgatgaaa agtataaaat tcagcctggc
    14821 ctggcagatt ctgtttgcta tggtgctggg cattctcctg ggaagctacc tgcactacca
    14881 tagcgacagc cgcgactggc tggtcgtcaa tttgctctct ccggcgggtg atatcttcat
    14941 ccatctgatc aaaatgattg ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc
    15001 gggtgttggt gatgccaaac agctcgggcg tattggcgcg aaaaccatta tttacttcga
    15061 ggtgatcacc accgtcgcca tcattttggg gatcactctg gcgaacgtct tccagcccgg
    15121 tgccggggtg gatatgtcgc agctggcgac cgtcgatatc tcgaaatatc agagcactac
    15181 ggaagcggta caaagcagtt cccacggcat tatgggcacg attttgtcgc tggtgccgac
    15241 gaacattgtg gcgtcgatgg cgaaaggcga aatgctgccg atcatcttct tctcggtgct
    15301 gtttggtctg gggctttcct ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt
    15361 ccgctccatc tctgaaacca tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt
    15421 gggtgtgttt gcgctgattg cggtgacggt ggctaacttt ggtttctctt ctctgtggcc
    15481 actggcgaaa ctggtgctgc tggtgcattt cgccattctg ttcttcgcgc tggtagtgct
    15541 gggaattgtg gcgcgcctgt gcgggttaag cgtctggatc ctgattcgta ttctgaaaga
    15601 tgagctgatt ctggcgtact ccactgccag ctctgaaagc gtactgccgc gaattattga
    15661 gaagatggaa gcctacggag caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta
    15721 ctcttttaac cttgatggtt cgacgctgta tcaaagtatt gccgctattt tcatcgctca
    15781 gctgtatggc attgacctgt ccatctggca ggaaatcatt ctggtgctga cgctgatggt
    15841 gacctcgaaa gggattgctg gcgtgccggg cgtgtcgttt gtggtgctgc tggcaacgct
    15901 gggtagcgta ggtattccgc tggaaggcct ggcgtttatt gctggtgttg accgtatcct
    15961 cgacatggcg cgtactgcgc tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc
    16021 caagtgggaa cacaaatttg accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg
    16081 caaatttgat aaaactgcgg atcaataatt gaagattgcc ggggttatcc acccggcaat
    16141 gtgtggatgc ctgatgcgac gctggtgcgt cttatcaggc ctaccttaac cgttggttaa
    16201 tttttcaaac tcttcacacc cggtatcaaa cccttccata cagctcaggt tcaaccagtg
    16261 cagcgccttt tgtttgttct tctcgataaa accttcctca ccgtttaaga acatcattcc
    16321 cgcccagtac tcggaataac cggttcggga aattgcagag ctgcgtttga aataccaggt
    16381 tgccttgtca tcgtcggcct taatgcccac gccgttggcg taaatcaaac caagcagcat
    16441 ttgggcatcg acggcggagt cattctccag atcttccgag gcgttttcta acagcgaaat
    16501 cgcctttgga taatccggtt tacccgcttg agtatttacc agaatatgcg ccagcgttac
    16561 ttcaccttct ttactgcccg cttgcgccgc tttctctgca agaacttttg cttgtggata
    16621 gtccagactg accggattgg tgattttgat ctgcgccagc agcgcgcagg catcagcatc
    16681 accgctgtcg gcggctttct gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa
    16741 ccagctgtcg gcaagaaaat attgtgcgcg tcggtccccg gcctctgctg cctttaaata
    16801 ctggctgcca ggctcggagt cgttcgcgtg ggcaaagaat gtcaaaaaca acatcaatgc
    16861 gataattttt ttcattttga taatacgatt gggtagggag taaccagtat aaagagttcg
    16921 aatggataaa aaaacagcct ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc
    16981 cgcttcgcgc aggcgagttt tcaacttgtt gtactcgtca atcacgtact gctcggcggc
    17041 gcgctgatcg gcgatcggct cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt
    17101 aatcgggctt aagttttcgg taaccagctc gttacaggca ccaatccacc actggtaggt
    17161 catgtaaatc gcccctttgt tcggacgatc gctgacctgc gcacgggtga taattttgcc
    17221 tttacgcgag tgcacccaaa ccaatgcctc atcttcaata cccagacgtt tggcgtcttc
    17281 ggtattgatt tgtgcgtagc caggttcatc agccagcgcc gccagtgccg cacagttacc
    17341 ggtcatcgaa cggcaagagt agtgaccaac ttcacgcacc gttgacagta ccatcgggta
    17401 ctcgtcggtg agtttgtcga ttggcgctac ccagtcgcag gtgaagaact gtgccagacc
    17461 gttcggggta tcaaacttct ctttaaacag ataagaagtc ccctgatcgg catctgaagt
    17521 atcgcggcaa ggccactgaa tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta
    17581 gaaatccggg cacagatgac gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat
    17641 cggataaccc atacgggtgg cgatttcact gatgatttgc cagtccgttt tcagatccca
    17701 tttcggttca actgctttga agaaacgctg gaagccacgg tcagccgcag taaacacgcc
    17761 ttcatgctcg ccccacgacg ttgacggcaa aatgacatcc gccgccgacg cggttttggt
    17821 cataaagatg tcctgaacga taaccagttc cagatcttca aaggctttac gtactgccga
    17881 cagctccgcg tcagtttgca gcggatcttc gcccataatg tacgcggcac gcacttcgcc
    17941 atgcgctgcg cggtgcggca gctcgctgat gcgataaccg gtatgagc
//
LOCUS       DAFATA010000092        17978 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFATA010000092 DAFATA010000000
VERSION     DAFATA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.093"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(851..1882)
                     /locus_tag="LGJ20_003654"
     CDS             complement(851..1882)
                     /locus_tag="LGJ20_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK2387668.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSDETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            2855..3118
                     /locus_tag="LGJ20_003655"
     CDS             2855..3118
                     /locus_tag="LGJ20_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBK2387669.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            3036..3323
                     /locus_tag="LGJ20_003656"
     CDS             3036..3323
                     /locus_tag="LGJ20_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HBK2387670.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            3765..4298
                     /locus_tag="LGJ20_003657"
     CDS             3765..4298
                     /locus_tag="LGJ20_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBK2387671.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            4552..5235
                     /locus_tag="LGJ20_003658"
     CDS             4552..5235
                     /locus_tag="LGJ20_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HBK2387672.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            5408..5959
                     /locus_tag="LGJ20_003659"
     CDS             5408..5959
                     /locus_tag="LGJ20_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387673.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            6121..6375
                     /locus_tag="LGJ20_003660"
     CDS             6121..6375
                     /locus_tag="LGJ20_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBK2387674.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            6551..6847
                     /locus_tag="LGJ20_003661"
     CDS             6551..6847
                     /locus_tag="LGJ20_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HBK2387675.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILASPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQYEVPNETHKNVVIENSIKSQLI"
     gene            7471..8538
                     /gene="pilL"
                     /locus_tag="LGJ20_003662"
     CDS             7471..8538
                     /gene="pilL"
                     /locus_tag="LGJ20_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HBK2387676.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWNNKTLTVTTSAAAPAQDTATTISPAAMVTTSTYPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            8538..8975
                     /gene="pilM"
                     /locus_tag="LGJ20_003663"
     CDS             8538..8975
                     /gene="pilM"
                     /locus_tag="LGJ20_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBK2387677.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            8989..10671
                     /locus_tag="LGJ20_003664"
     CDS             8989..10671
                     /locus_tag="LGJ20_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBK2387678.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            10664..11959
                     /gene="pilO2"
                     /locus_tag="LGJ20_003665"
     CDS             10664..11959
                     /gene="pilO2"
                     /locus_tag="LGJ20_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBK2387679.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            11946..12398
                     /gene="pilP"
                     /locus_tag="LGJ20_003666"
     CDS             11946..12398
                     /gene="pilP"
                     /locus_tag="LGJ20_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBK2387680.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPIIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            12409..13962
                     /gene="tadA"
                     /locus_tag="LGJ20_003667"
     CDS             12409..13962
                     /gene="tadA"
                     /locus_tag="LGJ20_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK2387681.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            13975..15072
                     /locus_tag="LGJ20_003668"
     CDS             13975..15072
                     /locus_tag="LGJ20_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBK2387682.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            15090..15704
                     /locus_tag="LGJ20_003669"
     CDS             15090..15704
                     /locus_tag="LGJ20_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HBK2387683.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            15714..16274
                     /locus_tag="LGJ20_003670"
     CDS             15714..16274
                     /locus_tag="LGJ20_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBK2387684.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            16259..16915
                     /locus_tag="LGJ20_003671"
     CDS             16259..16915
                     /locus_tag="LGJ20_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK2387685.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            16915..>17978
                     /gene="pilV"
                     /locus_tag="LGJ20_003672"
     CDS             16915..>17978
                     /gene="pilV"
                     /locus_tag="LGJ20_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBK2387686.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         4852 a         4364 c         4155 g         4607 t
ORIGIN      
        1 caggatatgc cgcgccagcg gcatggaagg cggcacgctg tggttacatg tgataccgga
       61 gtaaaaccgc cgaagcccgg cgtcagccgg tactgattga tagatttcac cttacccatc
      121 cccagccctg ctaagccata cccgctttca gccatgagac agtttctgtg cgcggtcgga
      181 gtggtcccga cgagggttta cccgaagtcg gggcgtatct ccgcgttagc gggccgtcag
      241 ggccgcttac gagcgtgtac tgagaacgtc cagccagaag actgacagcg atgaaaatgt
      301 agttacaaca ttcataatta aaagcgatgt tgttcccgcc ctttgggcgg gggcggcctc
      361 gcttttcagt tatgaggggt gggcacggtg ctgcgtttgt gtggtgtttt tattcctgat
      421 gtttttttgt tgtggtttca gaacggtgag gttcggggcc cgcgcgtcag cgtgggaggt
      481 gcttttagat tctgagcggc gcggccttgg ccgctgcaag cgaaggcctc tttgtatttg
      541 ttcacagtgg tctcagagat aaagattttc cctgcggcga agcaacgcag gggattaaat
      601 aatggggtta agactatatt gcaggtttgc tgtttcaggt ttgcagcttc ttaaagttct
      661 ttgtttgcgg atttatgtgg ataactgaaa caacgtggcc atgttctgat gactgatgcg
      721 tggttttatg ggcagatatg cccctctgag gggatttaag gtgattactg cggattttgg
      781 gctgagcgga accagcgccg aaagccccgg aggggctggt gatgattcag tgctgaggga
      841 atggtgctgt ttagtgcggt ggcggggcag ccagcgtcag ttcaagctga cgcaactccc
      901 tgatggccat tcgcttcaga tggtcatcgg aacaaaaata ggcttcatcc ggaggcagac
      961 gcttcctgag atactctgcc atctcatgaa tctgctggtc ggcaggcagt ttcttcaggc
     1021 gtctggcgcg cttactggct gctgctttcg cacgacggtg tttcagcgca tcctgactgc
     1081 gctgcagata ccagcgtttt ctggccgcat gtacggagat atcttcatcc tcacgcagaa
     1141 caccttcccg aatgagctgc tggcggattt cactttcacg cagtcgtttc tgctgctgtt
     1201 cgtgaagttt taccatgtcg acgcccagca tctgccagcc tgccggtgtt atccagacgt
     1261 agcgtggcag acgctggcgg gtttcacggt cccacagggt ttcatctgaa acacccagca
     1321 caccaaaacg tacctgttcg gccagcaaac gggaaaggcg ggagaccgtc acttccagtt
     1381 ccggaataac cttccccttg ctgtctttcg ggctgatttc tctggccaga cgggaaacac
     1441 tcatgcctac ggtatgtgtg cccgcatcac tgaagctgac cagaaccggc cagacggcat
     1501 cgaggaggcg ttgtttttcc ggacggaaag catatttgcg accggcatac gttcgtttct
     1561 gcacgtaaaa aggatgcaga gacatgtgca tgcgcaggct ctgctcgccg gtcaccgggt
     1621 cctttttcac cagacggttg taggcgtgcc cgagctgccc gctcagcgcc ttatagcgct
     1681 caggacgata ccaggacgga ttttcgcatt tgggtttggt ggagtgctca ccacgacgac
     1741 gcttcgtgcg cttcctttcc ggttcaacca ggaaagtggt cgcccttccc gcttcatagt
     1801 cttcccagaa acggatctgc tcttcgggtg ctaactgact ccagtgtact gcgttatatg
     1861 gagtattctt cagacctgcc acaagctgtc gcttatggcg gagcgattgt gccgtgtatt
     1921 tatacactgc acagggttga atctctggta cggtttcatt atactttacc cgtattctgt
     1981 gaggccccca ctatctttcg tcgttccgcc aagttcgtaa gaaagatagt gggggttttc
     2041 cttttatggc ttccggaaca tcttcagtct tctgacttac tgtccgtaca tgccatctct
     2101 cctgaatgcc ttatggcgca ttctgccgtc cggctttcgc cgctttgctc cacagagtgc
     2161 caccctctgc acagctaata acagccgaaa tatacccgct ttacgttaat ttgaaaagca
     2221 ttacaacgat ctcttctctt ccagtgcctc ctcactgata cctctaataa gttaatcttg
     2281 acctgcgtca aaaaaataaa atctattttt aaaagcagat ttttctgctt tagatctttc
     2341 ttttggatta aaagatcgac ttttgtccta ctcacgatca tttatcttac aaccgatcta
     2401 cccaacagaa taatattctg ttttaatgca attaaacgat ctaaataacc tttaaaaaat
     2461 cattttgtag atcattcgat cgtaaataga ataattctta gatgataaaa ccatttgtta
     2521 cacaacccgc caattgtgta acaacagcct ctacaagagc atatttgttc atatgttcgt
     2581 cagaactgcg gattgtctgg acgagggtca gacaccgttt cgtgcatatg gaccagcatg
     2641 ttcgaacgcc acaaagcaga agcatcatgc ctggatcgct tcggcgcgga gcgttaagtt
     2701 ttgacgcttt tttgtatgtt ttttaagctg ttagttgact cggtgcgtta tactaagtac
     2761 cctttactgg tcacaggcaa aactgtgatc gggcgtagga acccgcgtag tcatcgtcgg
     2821 cgcagaagtt aatttcttca tttggcgtcg ttttttgcca taccataaag acaggaactt
     2881 gtatttcttg tcgtcatcta tggtagctca ggcggggcag gcattagcct ggccggtttc
     2941 cgatgacgcc ggtattccta cccccgtctg ggctatcacc aatgagcgta ggaactcggt
     3001 gatagcgttt ttagttgtca tcggagacct ttgctatgtc acctcaactg atacagaaac
     3061 tcccggccat cactctgttg gaaggcatgt ttcccgagct atcaacaaac cagcttaaag
     3121 tctgcgtttt ttacgcaatg ggtgttcctt acgacgccat tgctcagaac tgtcggcttt
     3181 ctcccgaaac ggtacgaacc tacctgaagc gaagcctgaa gaatttaaat ctggaaggat
     3241 atgatgcact tcgctctgct gtgctgatgc gaactttcgt tttcatgatt agcaatacag
     3301 ctaaagaaaa cgaaaaaatg tgaacaggtg tccccattaa aggggacact ttcatggaaa
     3361 ttgcattttg cactttacag gcgatcccaa attgataaac ataaacacca agcacaagtt
     3421 actttaataa agcaaatcac ttcagacggt gtcccagtta tttgtatctg gctcatgccc
     3481 gatatatggc gaccttactc tgaactgatg tgctgatgtt ttatttcgcg tatcacaata
     3541 ctgtgccact gtattgctga tattagtgct tagccgttct ttatctgtga gttcgccaaa
     3601 tcaccgatac agaacgtact agagtctgct ggttatatgc ctgtcagaac tgcagtttat
     3661 ccagatatgg gctggacact gcttcagaca tatgatttag catattcgcc tgacatcgca
     3721 aaatcgtcca tcatggatcg gttttgcgcg gagcgttgaa cttcgtgaat atagaacacc
     3781 tgaataaccg gaactggtat cttgcgcaat ataatacagc cggcaaaaac agagaatctt
     3841 tattctcatg gctgaatgag caaaacgtcg taccatggac tccattaata acaagaaaga
     3901 taagacgggc agactccagg tgctgttacc gtgaaagaat attcgctatt tttccagggt
     3961 acttttttat tctggctaat tttgacattc aaccagtatc ggcattacgt cgccatagcg
     4021 ccttcattga tttcgtgaag tttggcggtg aaataaaacc cgtcaataaa gatattgttg
     4081 atggattaat gaagatttac ccggaccctg tgctaaatcc gggagccagg gaagagctga
     4141 atgcggcttc cagcatatgg ctgacaaaag cacaatatca gtatctcctc agaatggaaa
     4201 atactctcca gcctgagtcg aggatatccc tgctcctgga actggtatca aatgccgaac
     4261 atcacggctt tatggagcgc cttgtaaaca tcccatagcc agtatttccc ggccttcggg
     4321 ccgggctttt ttcaattcaa gagaatattc tggagtgaag cctgcacgga gccatcccca
     4381 ccatggactc cccctgtctt ttgatggaac cgtgtatttc ttaatggcga aagaaataaa
     4441 aggcttcacc ccagagtatt gattcgtttt atcaaaggcc ggcccgtgcc atcacgtgtg
     4501 gcggagagaa cggataccgg tcttcttttc atctcacagt aggacattcg catgaccacg
     4561 acagaacatc ccgtgcgcca gttgctctgg tgtgcactga atgccctgaa aacggcacag
     4621 gaaaaccaca atataaccag tgaaaccgga ttacggcatt acctgcttga atggctttct
     4681 ggtgccagca gacatcctga gttccgtagt ctccctggcg aaatcatggc gttaaaagca
     4741 ttgaccgaaa aagatcgcaa tattcccatc atcggaacgc tgaatacgct ctttctcagc
     4801 tctgccactg tcggggaatg tccgttgttt cgttttcggg ctgcactggg taaattacag
     4861 aaagcaggct ggcgcacata tgtctgccca tggcctgaga gggtgttcaa tgaatccatt
     4921 gaacgggcct gttccgggaa acgtcatctg ctacaactaa gtcgcaccga agagtgtttt
     4981 ctaccgacag gcgatatgag ggcaccagtt acgttgcagc tgatcactcc actacaccgc
     5041 aaaactatgc agcttctgga aacggcagag ttaatattct ccgacgaagg atttcaggtg
     5101 gtgagaggat acgaacatca attcgggatc gaacgcagag aaaccctctt tcacacccta
     5161 tttatcgggc tgcccagcct gccagaagat gtctggggtg caacacagga gcagacgact
     5221 atcacattac actgaggatg tgtaatcccc ggcatacccc gtttccagtc ttcgcagcct
     5281 gtaccgccct tcctgccgtt tccgacattc tttctgtcac tactcgcttg tgccattttt
     5341 tcgcaagcgg cccccatcga catgacggtg cgagtctttc tttttaccct gagaggaaat
     5401 taacagcatg aaaaaacaac cactactctg gatgacgata gccgccgcac tgctgtccgg
     5461 ctgcacaggc cacagggaac atcacccgca ggtgacattc acggtcgata ccgtgcgaac
     5521 agacacaacg gccagcaacc caatctgcct ggcggaaaaa tacggaagcc ttcttaatga
     5581 gcgccatccg gacctgtttg ccggtgctca ggggagtatt tcacgcagtg cattttttaa
     5641 tgacagcctg acgctgacac ctgaaacgct ccacgggatg atacagaata cgccctgtgt
     5701 tgaatcactg gtaacgtccg taggtccgga taccttcact gcagatgtct ctcccggtgt
     5761 cagaatgcag gtatcaccgg atattcacgg tgcgcccgga aatatcactc tgcgactctc
     5821 gaccacagac cagaaacgaa aacagactgt tgaacaaagc atttctctca gaccggaaca
     5881 gtcccttgtc gtgaatggat tcaccggaga cggagctggc gaaaagcgcg tcgttctcat
     5941 cacacctcac gtgcgttaat tcccaacaca acccgtttcc cgtcttcgca gcctgtaccg
     6001 cccttcctgc cgtttccgac attctttctg tcactactcg cttgtgccgt tttttcgcaa
     6061 ccggtacgtg cagttatgac ggtacagctg cttcttttcc cgttccggaa ggagtctttg
     6121 atgccacagc aacatcccgg acgactgcaa atactggtcg tcgacgcaca ctgcaaacgc
     6181 aggctgtttt caacgaaaac accgacagac cccgatgagc ttgcccgacg tttctgcacg
     6241 cccgataact gtctcgtggt ggtactacgc gacaaccgat ttctgttccg actggagcgt
     6301 gctcccggca gccactgccg ctggcacaag ggcagcagct cccgccatca gcatctacag
     6361 gactggctgt cgtaaacaca acaaaaacaa aagacctcgt caaaacaggt gggggccgtg
     6421 ttctttttag ttaaatttac catcgcgatt gcgattgtct gtgtacacct tttaacgatt
     6481 ttaatttagc cgtttgttgc catctttatt ttctttatta cctctctccc ttttatcatg
     6541 gagattaacg atgtttacca tgttctttct agttaaatta accatcgcga ttgtgattgt
     6601 ctgtggaatc ctttctccga ttttagcttc accacataac aaactgtcca cacttttttg
     6661 gaagatacca aacgcgttct ggattttcct gatttgcata tactttgcaa tcgccatggc
     6721 ggtgttaata gaaagcagtg aacagacacg catccgccgc caacaatatt tccaacagta
     6781 cgaagtgcca aatgagacac ataaaaatgt ggtgatagaa aacagtatta aaagtcaact
     6841 aatatgaaaa ataataacgt accattatat ctatggttgc tttggtgtac gctttgtctg
     6901 tacatcacta ccccatggca atacttacgt tatccataca ctgacaactt atcattgcct
     6961 tatatgttat atttctgtgt ggtggttgtg atttgcattt tcccactact tttcagatgt
     7021 gtacttctta ttgtaaggtc tgcatctaaa tacgacttca cattctttgt gaagaagaaa
     7081 aagggaagca agaaatcaac tatacatctt agcccctatg ctgacacacg ttgcccgaag
     7141 tcaatgagga aacactggga tgtattgctt tctctgatat caaaagcaat taataatgat
     7201 gaaatcattg tcatgcattc acatctcttt actccagcaa gaataaataa acttataaag
     7261 agactggaat cttatgattt gagtttttca gttattacat acccacggaa aacaagtctg
     7321 tacgaaaaat tatcaattcc ttttatatat ctcttatttc agtggtcttt cccgcatttg
     7381 cattcggacg gaatgacagt cgtcattcgt cctctccagc cgcctcacac caattaacaa
     7441 acacgtatat acgaacacgg agtctccgtt atgaaaaaaa actgtctgac acttatactg
     7501 ccgtcattat tgtccggctg tgccggaatt tccggcagca ataacgtggc accatcagat
     7561 acgcctgctc tggtttttgt tgacggacaa atcagtgaaa gtgctggcgt tctggcactt
     7621 acgcaacaac gtctttctcc acctaaaata actccccccc cgagagtcag cgtgccagag
     7681 acagtaaaaa cagcacagcc tgttagcgaa tcccgaccgt taccttcacc aacaataagt
     7741 cagaatttct cccggttaat cattacaggc aagcctggta gcgcccctgt aacactatcg
     7801 ccgacggccc gtaaccggac cgtcagtcag tggataaaaa cattactacc ggaaggatgg
     7861 aaattcagac aagagaatgc agccacgccc aagctgaata ccaaactggt ttcatggtct
     7921 gccaatgatc agtggacccg ttctctgaac cggttgctgg aagaacaaca cctctggggc
     7981 catctggact ggaataataa aacgttgacc gtcacaacat cagctgctgc accggcacag
     8041 gacacggcaa ccactatctc gccagcagcc atggtaacca catccactta ccctgaatct
     8101 ccaacgacag caaatagcca aaacaaacca cgaaatccat tcaggggaaa cagcgtatct
     8161 tcgtcaacgc ccgccgccac cccgactcca accggctcca cagtcaagtc tatcccgctc
     8221 atgaccggaa cgcctgttaa accggtatct cagggtaaag agtggcgtgc gccggcaggc
     8281 accacattaa gagagaacat tatcaagtgg gcagaagaaa caaaatgcga aagcatggca
     8341 tcaacgcact ggatggtcat atggccactt tctgtaaccg attaccgtct ggatgcgcct
     8401 ttaatgttcc ggggatcatt tgaatcagcc atggagcagg tatttgagtt atatcggact
     8461 gcgcaaaaac ctttatacgc acaggcttct cgcatgcagt gcatcattac agtttctgat
     8521 acaagggatg gtcgctaatg ggatggctgg tcatggccgt cggcctgacc attctgatta
     8581 tcactggtag ctaccagaat cagaagatgt ctgaaaccac aaacgcccag caatatgcaa
     8641 gtgcgtctgt atgggcaagt cagatactaa tgattgctaa cagaatcaat gatatcagat
     8701 acgtatcagg ccagcaggac ggagtaattt cctcagataa actggctctc cctgtcactc
     8761 cggactcccg aattaaacat cagttacaac aggggcgtct gtgggtatgg atgcctgaac
     8821 aaccaggtct tgttgaaact ttgcgttcaa aaagtcgtgg ttctgcacta atagggatct
     8881 tccaaaacgg gcaactcacc tggctatcag gaactgcaac cggtctgaca cccccggcag
     8941 gcattactgc tggttctgtc gtctacgtta actaatctgg atatattcat gaaaaaatca
     9001 caccagcgtt caatgaagct ggcggtgctc ccctgcatga tcgccgtcgc tctttccatc
     9061 tccggatgta ctttcagcga aatcaacaaa atgcagaaaa aagcacagga agactcagca
     9121 catgcacggg aaaaggtatc agccctttcg gcccgtaaat cgcaggctct tacctggctc
     9181 gataatcaat ggataaaccc tgttccggtc gctcaggtat caagagagaa aaaacaaaca
     9241 gctccggcct gctacatcac gcaggcaaga aaaggagaga tcactctgca ggaactgggg
     9301 caacgtatta ctgccgtatg cggcatccct gtgatcatca cgcctgacgc agccaattca
     9361 actcttgaag gaggcgctac ccgccagatg acaggaacac taccagcacc agatgaaaat
     9421 gggcgtctac cgttaagcag cctgggcagc acaacaatga ctacctccac tcagccatta
     9481 acgctgaata acctcatgtg gcagggagat atcaatggtc ttctggatct gatggccagc
     9541 cggagtggtc tgtactggcg catggataat ggtcggattg tattctatct gactgaaacc
     9601 agaacgtatc cacttcatat gctgaacacc aaaaccagca gcagttccag tgtcagctct
     9661 ggctcaacaa gcacaatggg ggcaacagga ggccaggata actcagcatc cggtgatgca
     9721 acgtcctctc agagcacaac cgttggtcag gaatacgatc tgtatgaaga catccggaaa
     9781 actattgaag caatgctgac accagaaaaa ggccgttact ggttatctgc atcgagctca
     9841 acgctgactg tcactgatac tccagctgtc caggaagccg tcgcacgata tgtggacgaa
     9901 caaaacagta ttatgaaccg ccaggtagcc ctgaacgtac aggttctgag cgtcagcaat
     9961 accagaaacg aacagttcgg tctggactgg aaccttgttt ataaatcgct acattccgcc
    10021 ggagcaacgt tgaacaatgc aagcggagat tttacaggcg ctacatctgc aggcgtatca
    10081 attctggata cggcaacagg gaatgccgcc aaattcagcg gttccagtct tctgattaaa
    10141 gcgctgagtg aacagggcga tgtcagtgtt gtgacttcac aagaaagcac tgtcacaaac
    10201 ctgacgccgg tacctatcca gatggcagat cagacggttt acgtcgccca gtcagcaaca
    10261 acaacgacta cggatgtagg agcaacaaca acattaacgc cgggcatgat caccaccgga
    10321 ttcaatatga ccctgctgcc tttaattcag aaaacgggca atctccagtt gcagatgaat
    10381 tttaatctgt cagatccccc aacaatccgt agctttacgt caaaagacgg aaacagttac
    10441 atcgaaatgc cgtataccaa actgcgttca ctgagccaga aggtcaatct gaaagaaggg
    10501 caatcacttg tcgttactgg tttcgatcag aacaatacga cgacaagtaa agccggtacg
    10561 tttacgccag caaatccatt atttggtggt tcacaaaccg ggaaaaatga acgcagcacg
    10621 cttgtaatca tcattacccc gactttcccg tcaggaggca acaatggctg atgaagatat
    10681 caacccggtt gtgcttctcg cagatcctaa agtaaatcat cgcgtgtggg cagcctgtct
    10741 taaatggagt cctgtcgtaa aaaaacagcg agtcccttct catcagaaac acaagcccca
    10801 tgtaaaatcc agacggctta cctctctgaa agtgactgta ggcagcagaa gttcccgtgg
    10861 gaagatcagc cgcattaccg gcacaggaat actggccagg ccggagcgca atcattattt
    10921 ttcccttgct ctggctttct gctcatgggt gagaaatgga tacggcgtct tccggtacag
    10981 cgataaggag cttttattcc ttgcctccat taatggccag cctgccgtta tggctgatct
    11041 gtcaggaaat gatgctgatg ttgctcaaaa ggtcagtctt tttctgacga tgaacgaaga
    11101 gccccccgaa aaatggcagg tcgtatctcc tctggaacat cctgataact gggaaagtat
    11161 tattacccgg ttatcatcag cagatctccg ccgttgcaaa cttactgtcg gcaatcgcag
    11221 caaattcaca ctgcctgccg tactgttttt agtagcagcc ggcgcaggca ctgtattctg
    11281 gatgactcag ccagagccgg atgtcgtacc gacgcccgag gagatagctg cacgggcaag
    11341 attgcagttt aaaaagcccg atccaccgcc agaactcccg catccatggg catcacaacc
    11401 tgtaatcagt gattttctga aagcctgcgc ggaccttcga aaaccatcac cggtcgcgct
    11461 agaaggctgg aaacttaccg gtggaacatg tactcccgag acattcacac tgatttacga
    11521 acgacagcca ggaggaacta ttgaaggctt tctggcaaga agcaaagaaa tctttaatgt
    11581 tattcctgac tttaacctta aagatggtgc acgcctggct tctgtaaccc ggccactgcc
    11641 ttccctgccc cgcagggatg aggcagtccc taccccctca gaacaactga tgcgggtgtt
    11701 cacgtggttt cagaaaaaac agttaacgcc ggccattaat gaaatagcca taccggaacc
    11761 attaccaggt aacgatggtg aaccagcccc tgttcagaaa tggaaagaat atcagttcag
    11821 tttatcaacg cctgtaaatc ctgatgagtt gttcccttta tttcaggata ccggcgttcg
    11881 tctgagcaat atccattttg aattaaacgg cggtactttc agttattcct cagaaggtca
    11941 tatttatgcg tccaggtaaa ttactcatta ttccatccat atttttcttc tccggttttt
    12001 catttgcaac aacacaacct cttgtcacaa taggagaact tgaagcacaa caaaaccgga
    12061 atattctttt acaggcaaaa gttcagggcg cacagctaca gaaacaactc gaagaatcag
    12121 atgttacttc atcatctgaa actgtttcag gtttctctgg tgtgactgcc tctttgccat
    12181 cagtttcaga gcagccgaca tctaaaaaca gaaaggaatt gcccatcatt atggaaataa
    12241 acggcaaaga taaacgtctt aatgccgttc ttcgcatggc tgatggacgt cagacaagcg
    12301 tcaccactgg cagccagctt ccaggcacat ccgtcacagt gaagtcgatc tcattgtctg
    12361 gcgtcacgtt aagtgatggc accaccctta ctttctgagt aacgtgatat ggataaatat
    12421 aatctgaagg atgctctcct ttttatatcc aggggagaca cccatgaaat tcttatagaa
    12481 acaaaccagc ggacaaggcc tgacgtacaa agtaatctcc aggagctgct aaaattatat
    12541 ccggatatca acccaaaagt agtctcgttg tcggagttac aggaggcagg tcaggatgag
    12601 aaaaacagag gcccgaaaga acgaattatt catctgaaag accttgctga tgtcagtgaa
    12661 agtgagaaga aagttctatc ttattttgaa actgccagaa aacttggtgc ttcagatatt
    12721 catttcttaa tttcagagtc tattttcaaa gttcgcatga gaatattcgg tgaactgcag
    12781 actgtagatg aagatcaacc agcactgggc tattcacttt gcgcaacagc cattctgtca
    12841 atggctgatg tcactgaaac cagtttcttc cctcaaaggg agcaggatgc gcgcctgtcc
    12901 ccacaattaa tgcgaaaaat aggaatattt ggtgcacgct acagtcacag acctacggga
    12961 gacggtctga ttgcagttat gcgcctgata cccgatgacg gagataaagt tcccacgttt
    13021 aaacaactgg gctttatccc ggaacaaatc cggttgctga acataatgct gcgccgcccg
    13081 gaaggaaaaa tagtcctgtc aggtccaacc ggttccggta aatcgacaac gttacgcagc
    13141 gcctgccgtg tatatcttga cgataatcag gggcgacacc tgctaacaat cgaagatccg
    13201 ctggaaggac agatactggg agcaacacag actccgatta tctgcgacaa atccgacgaa
    13261 gatgccgtca aactggcatg gagtcgggct atttcatcgg caatgcgact tgaccctgat
    13321 gccatcatgg aaggggaaat gcgcgatctt atctcgatga tgtcaaccac ctacgcggca
    13381 cagaccgggc acatagttct gacgacatta catacaaact cagctctggg tataccggag
    13441 cgaatgatta cgatgggtat gaatgccgat cttatttgcg atgcgcaatt actgattggg
    13501 atgatcagcc agcgccttgt accaactctg tgtccctcct gccgtattcc atgggaaaag
    13561 cgagcacctg aacttagcga cgacgagcgt gattaccttg agcggcactg caataaagat
    13621 tcactctgca gtacagataa tatctggttc cgaaatcatc acggatgctc tgaatgtaat
    13681 cacgacgtaa tcattaacgg cagaaagcgc ggtgaaatag gtaaaggcct caccggcagg
    13741 actgtaattg ctgaggtgat agaacctgat aaccgcttat ttcagattct gaaaacacgc
    13801 ggcaaagttg cagcacgtaa atactggctg gaaaacatga aaggcatcag tcgtgtagag
    13861 catcttcttc gccgtatcaa tgaaggtctg gtagaccctc ttgaagccga cagaatcatc
    13921 cctctggacg aagacgagcg cctcagcatc gatgatgtct aacaggagaa caacatgcgt
    13981 gaaatgaatt tcagtcaacg gctgaggcgt ttcattgtca gaaaaacgtt cagcgccccc
    14041 tacagggtgc agttttacga agccctgcgt tttctgctgg aaaataaaca gccattaaag
    14101 accgcactgg aacagatgcg ggatgcctgg acagactttg gacgaaaatg gcatcccttt
    14161 gctgaactgg ccacagactg cattgagtct cttcgtgaaa acagtggcga aaactctctg
    14221 gaatatactc ttagtttatg ggttcctcag gaagaggccg cagtaatcag cgccgggata
    14281 agaagtggtt caattgtgga tgcactgcaa ttcgccacca ctcttacaga tgcaaaagaa
    14341 caaatccatc aggctatatg gcaaatggct atttatccgg tgggattgtt aatcatgatg
    14401 acggggacat tatatgttct gaatacagag ctaatacctg aactgagcaa aataagctcc
    14461 cccgattcat ggagtggtgc tcttggtttt ctttatggat tatctgtttt tgtcgataat
    14521 tacggcgcta tatgtgccgt tctttttgca gtgatcacag gtttaatatc atggtctctg
    14581 ccaaactgga aaagccctga cagtgttcgt acttttgccg acaagataat gccatggagt
    14641 atttatcagg atatacaggg agcaacattt ctcctgaata tggctgcact tctgaaagcc
    14701 aaaatgacga cgctcaactc gctaaacatt cttcaggagt ttgcatcacc atggttaagc
    14761 acacgtcttg acagcattat ttaccgcgtc cgccaggggg atcatcttgg actggctttg
    14821 cgtcagtgcg gttatcagtt cccatccagg gaagcagcca acttcctgtc tttattacag
    14881 ggagatggcg caacagagct aatcagcaat tatggtcaac gatggcttag tcaaacactt
    14941 caacgtgtaa agaaacgggc aaacgtgatt cgtttaatta tgctgatatt tcttgtcatg
    15001 tctctcatgt tactggtttt tgccattatg gatattcagt ctattagtga caacagcatg
    15061 ggtaactttt aaacaaaacg aggatattta tgttagtaga aaatattaat accactttaa
    15121 ctggaaataa taagaagaat gagcctcatg ataaaggatg ggcaattttg gagcagggaa
    15181 caatagcgct cgttgttctt tttgttatag tcgttgttct gggtagtttg tatgcgttgc
    15241 gtacaagagc caatgtggca actgaaaccg caaatataca aacaattatc acaagtgccc
    15301 agagcctgct aaaaggtagc gatggatata catttaccag cagtgccaaa atgacaggtg
    15361 ccctcattca gatgggtgtc attccctctg ggatgaccgt acagggcgac aaaacatctg
    15421 gtacagcaac gctctataac gcatggggcg gtgctgtaac tgttgccccg gcttcaacat
    15481 cagggttcaa caacggtttt accgttacct atgataaagt cccacaggat gcctgtatcc
    15541 agattgccac acgaatcagt aaaactggtt taactaacgg cattaccctg aacagtactg
    15601 ctcacagtga tggaaaagtc actactgaag aagccagtac gcaatgcaag gcagataatg
    15661 gcagcactgg tacaaacaaa cttatcttca ccatcaatgg ttaaggagtt ttaatgtcag
    15721 ggggtacagt acccctcaga cttatttcat taatcctgct tttttctctt attccacccg
    15781 cccgagcttc atacgtagct cccaaacaga tctggaccaa caagtgggac aattgctttg
    15841 ccgctgccgg cgcgcgctat caaattgaac cattattatt gaaggctatt tctgccgggg
    15901 aatcttcgtt gaggccgggc gcaattaata taaataagga caaaaaaaca ggtaaagcca
    15961 gtagcacgga ttatgggttg atgcaaataa actcaaccca tattccaaag ctgataaata
    16021 tgggcgtaat aaaaaagtct gaggatttaa ttaccaaacc atgccttaat atccatatag
    16081 gaagctggat actggcaaga cattttcaga tatgtggcgt cagctggaat tgccttgggt
    16141 catacaatgc cggattccgt aaggacagac atgaaacaag agagcaatat gccaataaaa
    16201 tatggagaat ttatcgtgac atgaaaggga tatgtcttcc cggacaggga ggcaggcaat
    16261 gcagacaatc gtaggcataa gtctatcccc tgtttatata ctgccattga tgttcacatt
    16321 ttccattatt ggacgaactc tgctattcag agttcgttat tttttatctg atacgggaca
    16381 tctctggtat aagacacacc ctgctgttct gtcaggaata tggctataca gtatagctgt
    16441 ggcgttaatt attttatcat catcaccact gctctaccgt attcatgctg tccttattct
    16501 ttcgttcata ctgcaaatgg cggtaacgga tgcactgaca ggtttattac caggaacatt
    16561 cacccgacgt tttttaattg caggaatgct atcacaaatc acgactgata tatggtggtt
    16621 ccgtacaaca gaatttgcca ctgctgcaat tgtcttattc tgccttcata aattagttaa
    16681 ccgccatcgt cttaatattg gcactggaga cctgtggctt attgcaggta ttaccgcatg
    16741 gtcagggtta tataacgcca tatggtgtgt gttgctgggc acaggaggat ttgtcttatg
    16801 gcattcaaca tggtgcataa aagggcataa agaaggccct ctgggtccat ggctatgttt
    16861 tggccatgtc cttttgctgc tggataatct ttatcaacca ctatgggtaa tctgatgaaa
    16921 aaatatgacc gaggctgggc atcgctggaa actggcgcgg ctttactgat tgttatgctc
    16981 ctgattgcct ggggagcggg catatggcag gactatattc aaacaaaagg atggcaaact
    17041 gaagcacgtc ttgtcagcaa ctggaccagt gcggcccgct cttatatagg gaaaaactac
    17101 acgactctcc agggcagtag taccacaaca actcctgccg ttatcacgac aaccatgctg
    17161 aaaaataccg gctttttgtc cagcgggttt actgagacaa acagcgaggg gcagcggtta
    17221 caggcatatg tggttcgaaa cgcccaaaac ccggaattac tacaggcaat ggttgtatcc
    17281 agtggtggca caccttatcc agtgaaagca cttatccaga tggctaagga tattaccact
    17341 ggtcttggtg gatatatcca ggacggcaaa acagccacag gtgcattacg ttcctggtca
    17401 gtagctttaa gtaattatgg tgccaaaagc ggtaacgggc atattgccgt attgttatcg
    17461 acagatgaac ttagtggtgc agctgaggac actgatcgtc tttacagatt ccaggtcaat
    17521 ggtcgccctg acttaaacaa aatgcacacg gccattgata tgggatcaaa taacctgaat
    17581 aacgttgggg cagtaaatgc ccagacaggt aatttcagcg gcaatgtgaa tggtgtaaat
    17641 ggcactttca gcggtcaggt aaaaggcaat agcggaaact ttgacgtaaa tgtcaccgct
    17701 ggcggtgata tcagaagtaa taatggttgg ttaattactc gtaacagtaa aggctggctc
    17761 aatgaaactc acggtggcgg attttatatg tccgatggat catgggttcg aagtgtaaac
    17821 aacaagggca tctataccgg cggtcaggtg aaaggcggta ctgttcgggc tgatggtcgc
    17881 ctttatactg gtgaatactt acaactggaa agaactgccg ttgctggcgc atcatgttcg
    17941 cctaacggcc ttgtaggccg cgataataca ggggcaat
//
LOCUS       DAFATA010000093        17485 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFATA010000093 DAFATA010000000
VERSION     DAFATA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.094"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..681
                     /gene="phnF"
                     /locus_tag="LGJ20_003673"
     CDS             <1..681
                     /gene="phnF"
                     /locus_tag="LGJ20_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBK2387687.1"
                     /translation="EIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQL
                     VEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHV
                     ADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQRFDNGSLHDFLREQT
                     GIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRAD
                     MIEFTMEH"
     gene            682..1134
                     /gene="phnG"
                     /locus_tag="LGJ20_003674"
     CDS             682..1134
                     /gene="phnG"
                     /locus_tag="LGJ20_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBK2387688.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            1131..1715
                     /gene="phnH"
                     /locus_tag="LGJ20_003675"
     CDS             1131..1715
                     /gene="phnH"
                     /locus_tag="LGJ20_003675"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBK2387689.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            1715..2779
                     /gene="phnI"
                     /locus_tag="LGJ20_003676"
     CDS             1715..2779
                     /gene="phnI"
                     /locus_tag="LGJ20_003676"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBK2387690.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            2772..3617
                     /gene="phnJ"
                     /locus_tag="LGJ20_003677"
     CDS             2772..3617
                     /gene="phnJ"
                     /locus_tag="LGJ20_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBK2387691.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            3614..4372
                     /gene="phnK"
                     /locus_tag="LGJ20_003678"
     CDS             3614..4372
                     /gene="phnK"
                     /locus_tag="LGJ20_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBK2387692.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            4483..5163
                     /gene="phnL"
                     /locus_tag="LGJ20_003679"
     CDS             4483..5163
                     /gene="phnL"
                     /locus_tag="LGJ20_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBK2387693.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            5160..6296
                     /gene="phnM"
                     /locus_tag="LGJ20_003680"
     CDS             5160..6296
                     /gene="phnM"
                     /locus_tag="LGJ20_003680"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBK2387694.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            6296..6853
                     /gene="phnN"
                     /locus_tag="LGJ20_003681"
     CDS             6296..6853
                     /gene="phnN"
                     /locus_tag="LGJ20_003681"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBK2387695.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            6840..7274
                     /gene="phnO"
                     /locus_tag="LGJ20_003682"
     CDS             6840..7274
                     /gene="phnO"
                     /locus_tag="LGJ20_003682"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBK2387696.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            7276..8034
                     /gene="phnP"
                     /locus_tag="LGJ20_003683"
     CDS             7276..8034
                     /gene="phnP"
                     /locus_tag="LGJ20_003683"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBK2387697.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(8181..8510)
                     /gene="yjdP"
                     /locus_tag="LGJ20_003684"
     CDS             complement(8181..8510)
                     /gene="yjdP"
                     /locus_tag="LGJ20_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBK2387698.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDLERRQLEDEERR
                     MEDEYGR"
     gene            complement(8579..8896)
                     /locus_tag="LGJ20_003685"
     CDS             complement(8579..8896)
                     /locus_tag="LGJ20_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBK2387699.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            9149..11134
                     /gene="yjcS"
                     /locus_tag="LGJ20_003686"
     CDS             9149..11134
                     /gene="yjcS"
                     /locus_tag="LGJ20_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBK2387700.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            11343..11618
                     /locus_tag="LGJ20_003687"
     CDS             11343..11618
                     /locus_tag="LGJ20_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387701.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            11637..12668
                     /gene="mdtN"
                     /locus_tag="LGJ20_003688"
     CDS             11637..12668
                     /gene="mdtN"
                     /locus_tag="LGJ20_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBK2387702.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASTMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            12668..14719
                     /gene="mdtO"
                     /locus_tag="LGJ20_003689"
     CDS             12668..14719
                     /gene="mdtO"
                     /locus_tag="LGJ20_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBK2387703.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            14716..16182
                     /gene="mdtP"
                     /locus_tag="LGJ20_003690"
     CDS             14716..16182
                     /gene="mdtP"
                     /locus_tag="LGJ20_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBK2387704.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            16380..>17485
                     /locus_tag="LGJ20_003691"
     CDS             16380..>17485
                     /locus_tag="LGJ20_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16797..16799,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBK2387705.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKA"
BASE COUNT         3927 a         5031 c         4852 g         3675 t
ORIGIN      
        1 gagatagccg caaaacttga gcaggagctt cgtcaacact accgctgcgg cgactatctt
       61 cccgccgagc agcaactggc ggcgcgcttt gaggtgaacc gccacaccct gcgccgcgcc
      121 atcgaccaac tggtggaaaa aggctgggta cagcgccgtc agggcgtcgg cgtgctggtg
      181 ctgatgcgcc cgttcgatta cccgctcaac gcccaggcgc gttttagcca gaacctgctg
      241 gaccagggta gccatcccac cagcgaaaaa ctgctgtcgg tattgcgccc cgcgtccggt
      301 cacgtcgctg acgcgctggg gattaccgag ggcgagaacg ttatccattt gcgcaccctg
      361 cgtcgcgtca acggcgtcgc gctctgttta atcgaccact actttgcgga cctcaccctt
      421 tggccgacgc tgcaacgctt cgacaacggt tcgctgcacg attttctgcg cgagcaaacc
      481 ggaattgcgc tgcgccgcag ccagacgcgg atcagcgccc gtcgcgccca ggccaaagag
      541 tgccagcgtc ttgaaattcc gaatatgtcg ccgctgctgt gcgtgcgcac ccttaaccac
      601 cgtgacggtg aaagcagccc ggcggagtac tccgtcagcc tgacgcgcgc cgacatgatt
      661 gaattcacta tggagcactg aatgcacgca gataccgcga cccgccagca ctggatgtcc
      721 gtactggcgc acagccaacc ggctgaactg gcagcccgcc tgaaagcact gaacatcacc
      781 gccgactatg aggttatccg cgccgccgaa accggcctgg tgcagattca ggcgcggatg
      841 ggcggcaccg gcgaacgctt ttttgccggc gacgccacgc tcacccgcgc cgccgtacgc
      901 ctgactgacg gcacgctcgg ctacagctgg gtgctggggc gcgacaaaca gcacgccgaa
      961 cgctgcgcgc tgattgacgc gctgatgcag caatctcgct attttcaaaa cttatcagaa
     1021 acccttattg ccccgctgga cgctgaccgt atggcacgca ttgccgcacg ccaggccgaa
     1081 gtgaacgcca gccgggtcga cttctttacg atggttcgcg gagacaacgc atgaccctgg
     1141 aaaccgcttt tatgcttccc gtgcaggatg cccagcacag ttttcgtcgc ctgttaaagg
     1201 ccatgagcga gccgggcgtg attgtcgccc tgcatcagct caagcgcggc tggcagccgc
     1261 tgaatatcgc caccaccagc gtgctgctga cgctggccga taacgacacg ccggtgtggc
     1321 ttgccgcgcc attgagtaac gatatcgtca gccagagcct gcgttttcat accaacgcgc
     1381 cgctggtcag ccagccggaa caggcgacct tcgcggtgac ggatgaggcg atttccagcg
     1441 aacagcttaa cgccctttcc accggcaccg ccgttgcgcc ggaagcgggt gcaacgctga
     1501 ttttacaggt cgccagcctg agcggcgggc gcatgttacg tctcaccggc gcgggcattg
     1561 ccgaagaacg aatgatcgct ccgcagctgc cggagtgcat tctgcacgaa ctcaccgagc
     1621 gcccgcaccc gttcccgctc ggcatcgacc tgatcctgac ctgcggcgag cgcctgctgg
     1681 ctattccgcg aaccactcat gtggaggtgt gctgatgtac gttgccgtga aagggggcga
     1741 gaaggcgatc gacgccgctc acgccctgca agagagccga cgccggggcg ataccgattt
     1801 gcctgaactg agcgtcgccc agattgaaca gcagcttaat cttgcggtgg atcgcgtgat
     1861 gaccgaaggc ggcattgccg accgcgaact ggcggcgctg gcgctgaaac aggccagcgg
     1921 cgataacgtc gaagcaattt tcctgctgcg cgcctaccgc accacgctgg cgaagctcgc
     1981 ggtaagcgag ccgctcgaca ccacagagat gcgtctcgaa cggcgtattt cggcggttta
     2041 taaagacatt cccggcggcc agctgcttgg cccaacctac gactacaccc atcgcctgct
     2101 cgatttcacc ctgctggcaa acggtgaagc gccgacgctg accaccgccg acagcgaaca
     2161 acagccgtcg ccgcacgttt tcagcctgct ggcgcgtcag gggctggtga agtttgaaga
     2221 agatagcggc gcacagccgg atgacatcac ccgcacgccg ccggtttacc cctgctcacg
     2281 ctcctcccgc ctgcaacaat taatgcgcgg ggacgaaggc tatttgctgg cgctggccta
     2341 ctccacccag cgtggttacg ggcgcaatca cccgttcgcg ggcgagatcc gcagtggtta
     2401 catcgacgtg tcgattgtgc cggaagagct gggatttgcg gtaaacgtcg gcgaactact
     2461 gatgaccgag tgtgaaatgg tcaacggttt tatcgacccg ccggatgagc cgccgcactt
     2521 cacgcgcggc tacgggctgg tattcggcat gagcgagcgc aaagcgatgg caatggcgct
     2581 ggtcgatcgt gcgttgcagg ctccggaata cggcgagcac gcgacaggcc cggcgcagga
     2641 tgaagagttt gtgctggcac atgccgacaa cgtcgaagcc gcaggctttg tctcgcacct
     2701 caaactcccc cactacgtcg atttccaggc cgaactggag ctactcaaac gtctgcaaca
     2761 ggagaagaac catggctaat ctgagcggct acaactttgc ctacctcgac gagcagacca
     2821 aacgcatgat ccgccgcgct atcttaaaag cggtggcgat ccccggttat caggtgccgt
     2881 ttggcgggcg cgagatgccg atgccatacg gctggggaac cggcggcata cagctcaccg
     2941 ccagcgtgat tggcgaaagc gacgtgctga aggtgattga ccagggtgcg gatgacacca
     3001 ccaacgccgt gtcgattcgc aacttcttta agcgcgtgac cggggtaaac accactgaac
     3061 gtacggacga tgcgacgctt atccagacgc gtcaccgcat ccccgaaacg ccgctgaccg
     3121 aagatcagat cattatcttc caggtgccaa tcccggaacc gctgcgcttt atcgagccgc
     3181 gcgaaacgga aacccgcacc atgcacgcgc tggaagagta cggcgtgatg caggtgaaac
     3241 tgtatgaaga tatcgcccgc ttcggtcata tcgccactac ctacgcctat ccggtgaagg
     3301 tgaacgggcg ctacgtaatg gacccgtcgc cgatcccgaa attcgataac ccaaaaatgg
     3361 acatgatgcc cgccctgcaa ctgttcggcg cggggcgcga gaagcgcatc tatgcggtgc
     3421 cgccgtttac ccgcgtggaa agtctcgatt tcgacgatca cccgttcacc gttcagcagt
     3481 gggatgagcc atgcgccatc tgcggatcga cccacagcta tcttgatgaa gtggtgctgg
     3541 atgacgccgg aaaccgcatg tttgtctgct ccgataccga ttattgccgc caacagagcg
     3601 aggcgaaaag ccaatgaatc aaccgttact ttcggtcaat aacctgaccc acctttacgc
     3661 gccgggcaaa ggctttagcg atgtctcttt tgatttatgg ccgggggaag tgctgggcat
     3721 tgtcggggag tccggctccg ggaagaccac gctgctgaaa tcaatctccg cgcgcctgac
     3781 gccgcagcag ggggagattc actatgagag ccgttcgctg tacgcaatga gcgaggccga
     3841 ccgccgtcgc ctgctgcgca ccgaatgggg cgtagtgcat cagcatccgc tcgacggcct
     3901 gcgccgccag gtgtcggcag gcggcaatat cggcgagcgg ctgatggcga ccggggcacg
     3961 tcattacggc gatattcgtg ccaccgcgca gaagtggctg gaagaggtgg aaattcccgc
     4021 caaccggatc gacgacctgc cgaccacctt ttccggcggc atgcagcaac gtttgcagat
     4081 tgcccgcaac ctggtgacgc atccgaagct ggtgtttatg gatgaaccga ccggcgggct
     4141 ggatgtgtcg gtgcaggccc gcctgctcga cctgctgcgc ggcctggtgg tggagctgaa
     4201 cctggcggtg gtgattgtca cccatgattt aggcgttgcc cgcctgctgg cggaccgttt
     4261 gctggtgatg aagcaggggc aagtggtgga gagtgggtta accgaccgcg tgctcgacga
     4321 cccgcatcat ccgtatacac agctgctggt gtcatcggtt ttgcagaatt gagccggtgc
     4381 cggatgcggc gtaaacgcct tatccggcct acaaatgcgc tccccgtagg tcggataaga
     4441 cgcgtcagcg tcgcatccga cacccgaacc acgaggcgaa aaatgattaa cgtacaaaac
     4501 gtcagtaaaa ccttcatcct gcaccagcaa aacggcgtgc gcctgcccgt cctcaaccgc
     4561 gcctcgctca ccgtcaacgc gggcgaatgc gtggtgctcc acggtcactc cggcagcggc
     4621 aaatcaaccc tgctgcgctc gctgtacgcc aactatctgc ccgatgaagg tcaaatccag
     4681 atcaaacacg gtgacgagtg ggtagacctg gtcaccgcgc cagcccgcaa agtggtggag
     4741 atccgcaaaa ccaccgtcgg ctgggtgagc cagtttctgc gcgtcatccc gcgtatttca
     4801 gcactggaag tggtgatgca gccgctgctc gataccggcg ttccgcgtga agcctgcgcc
     4861 gctaaagccg cgcgtctgct cacccgcctg aacgtgccgg aacgcctgtg gcacctggcg
     4921 ccatcaacct tttccggtgg cgaacagcag cgcgtcaaca tcgcccgcgg ctttatcgtc
     4981 gactacccca ttctgctgct cgacgaacct accgcctcgc tggacgccaa aaacagcgcc
     5041 gccgtagtcg agctgattcg cgaagccaaa acccgtggcg cggccatcgt cggcatcttc
     5101 catgacgaag ccgtacgtaa tgacgtcgcc gaccgcttgc acccaatggg agcctctgca
     5161 tgattatcaa taacgtcaaa ctggtgctgg aaaacgaagt ggtacacggt tcgctggagg
     5221 tgcaggatgg cgaaatccgc gcctttgccg aaagccagag ccgcctgcca gaagcgatgg
     5281 acggcgaagg tggctggcta ctacccggcc tgattgagct gcataccgac aacctcgata
     5341 aattcttcac cccgcgcccg aaagtcgact ggcccgccca ttcggcgatg agcagccacg
     5401 acgcgctgat ggtggcaagc ggcatcacca ccgtgctgga cgcggtggcg attggcgacg
     5461 tgcgcgacgg cggcgatcgg ctggagaatc tggagaagat gatcaacacc atcgaagaga
     5521 cgcagaaacg cggcgtcaac cgcgccgagc accgtctgca cctgcgctgc gagctgccgc
     5581 atcacaccac actgccgctg tttgaaaaac tggttcagcg cgaaccggtg acgctggtgt
     5641 cgctgatgga ccactcaccg ggccagcgcc agttcgctaa ccgcgagaag tatcgcgaat
     5701 attatcaggg caaatactcc ctcaccgatg cgcagatgca gcagtacgaa gaagagcaat
     5761 tggcgctcgc cgcacgctgg tcgcagccga atcgcgaatc catcgccgcc ctgtgccgcg
     5821 cgcgacaaat tgcgctcgcc agccacgatg acgccacgca cgcccacgtt gccgaatccc
     5881 accaacttgg cagcgtgatc gccgaatttc ccaccacgtt cgaagcggcg gaagcctcgc
     5941 gtaagcatgg catgaacgtg ctgatgggtg cgccaaatat cgtgcgtggc ggctcgcact
     6001 ccggcaatgt ggcggccagt gaactggcgc agcttggtct gctggatatc ctctcttccg
     6061 actactaccc agccagcctg ctggatgcgg cgttccgcgt cgccgatgac gagagcaacc
     6121 gctttacgct accgcaggcg gtgaggctgg tgaccaaaaa tccggcgcag gcgctgaatc
     6181 ttcaggatcg cggggtgatt ggcgagggta aacgcgctga cctggtgctg gcgcatcgcc
     6241 agggcaatca cattcatatc gaccacgtct ggcgtcaggg taaaagggtg ttctgatgat
     6301 gggaaaactg atttggttaa tggggccgtc cggctccggg aaagacagcc tgctggcgga
     6361 actccgcctg cgggaacaaa cgcagttact ggtggcgcat cgctacatca cgcgcgacgc
     6421 cagcgccgga agtgaaaacc atatcgccct gagtgagcgg gagtttttta cccgcgcggg
     6481 gcaaaacctg ctggccttaa gctggcacgc taacggtctg tattatggcg tcggcatcga
     6541 gattgacctc tggctgcacg ctggattcga cgtagtcgtc aacggttcac gcgcgcattt
     6601 gccgcaggca cgggcgcgct atcaatcggc gctgctgccc gtctgtttac aggtttcgcc
     6661 ggagatcctg cgccagcgcc tggaaaaccg tggccgtgaa aacgccagtg aaattaacgc
     6721 ccgcctggcg cgcgccgccc gctatactcc acaggattgc catacgctca acaatgacgg
     6781 cagcctgcgc cagtcggtcg acacgctgct gacgctgatc catcagaagg agaaacacca
     6841 tgcctgcttg tgagcttcgc ccggccacgc agtacgatac cgacgcggtt tacgcgctga
     6901 tttgtgagct aaaacaggcg gagtttgacc accatgcgtt tcgcgtgggc tttaacgcca
     6961 acctgcgcga cccaaacatg cgctaccatc tggcgctgct ggatggcgaa gttgtcggca
     7021 tgatcggcct gcatttgcag tttcatctgc atcatgtcaa ctggatcggc gaaattcagg
     7081 aactggtggt gatgccgcag gcgcgcggtc tgaacgtcgg cagtaaatta ctggcatggg
     7141 cagaagaaga agcccgccag gccggggccg aaatgaccga gctttccacc aacgtgaagc
     7201 gccacgacgc acaccgtttt tacctgcgtg aaggctacga gcagagccac ttccgcttca
     7261 ccaaggcgct gtaacatgag cctgaccctc acgctcaccg gcactggcgg cgcacagggc
     7321 gttccggcat ggggctgcga gtgcgcggcc tgcgccagag cgcggcgctc gccgcagtat
     7381 cgccgccagc catgcagcgg cgtagtgaag tttaacgacg cgatcaccct gatcgacgcc
     7441 gggcggcacg atctcaccga tcgctggtcg cctggatcgt tccagcagtt tttgctgacg
     7501 cactaccata tggatcacgt ccaagggcta tttccgctac gctggggcgt gggcgatgtg
     7561 atcccggtct acggcccgcc ggatgaacag ggctgcgacg atctgtttaa acatccgggc
     7621 ctgcttgatt tcagccacac agtggaaccg tttgtggtgt ttgatttgca ggggttgcag
     7681 gtcacgccgc tgccgcttaa ccactcaaaa ctgaccttcg gttatctgct ggaaacggca
     7741 catagccggg tggcgtggct gtctgacacc gctggcctgc cggaaaaaac gctgaaattt
     7801 ttactcaata atcatccgca ggtgatggtg attgattgca gtcacccgcc gcgcgcggat
     7861 gcaccgcgta atcactgtga tttaaatacc gtgcttgcgc tgaatcaggt tatccgctcg
     7921 ccacaggtga ttctgaccca tatcagccac cagtttgatg cgtggctgat ggaaaacgca
     7981 ctaccgtcag ggtttgaggt ggggtttgat gggatggaga ttggggtggc gtgatgagag
     8041 ggaatgtgcg cgctggcccc ctcaccctaa ccctctcccc agaggggcga ggggaccgat
     8101 tgtgctcaat attgaatatt gcgctcgttt tctccctctc cccattgggg tgaggggcga
     8161 tgcctgctcc atacccaacc tcatcgccca tactcatctt ccattctccg ctcttcatcc
     8221 tccagttgcc gacgctccag atcaagctgg cgctggcgat cgtccagctg cctgcggcga
     8281 tcttcaaact ggcggcggcg gtcgtcatat tgtctgcgcc gatcgtcgct cacttcacgc
     8341 tgccagccgt cgtcgcgcga atcttcatag tctcgcccac ggtcagggtt ataagcgtca
     8401 ttaatcgcct gctgaatatt accaatagta tcgtcgataa tatcagcctg ggccggaacg
     8461 gtggacagcg tgagcagggt gaataaaaga aatagcggaa agcgtttcat tagccaacct
     8521 caaaaagaaa ctctatccac attagtcatt actcatccat gcaagtagtg gatgaatctc
     8581 aatttctccg ctgctctatt gccgtaatcg cctccacgcg ttgttgatga cgaccgcctt
     8641 cgtactgtgc gcccagccac gcatccacaa tcatttttgc cagttcgagg ccaaccactc
     8701 gtgaaccaaa agccagtgca acttcactgg cataatgcgg ataatcagtg cgctctgacg
     8761 acctggtgtc tttatcaatc acctcactca ttgaccatga ataaaaggct ggcacagcaa
     8821 cgcagaatcc tgcggtgcca gcgacatgac cgcccattgc tccggtacgt tttttaaaaa
     8881 gaaacaccgg ttgcattgat aaattgtgct cagtatttgc cgaagtacaa tatatataat
     8941 aaatatataa aacacctcat attatttaca gagataataa attcgcgtaa ttttccccct
     9001 tcagcacaaa tgcctaataa aatggatcgt tccgctcaca cttccactta ttaaagtcga
     9061 aaattcccaa ttatgataac cccagcttta aatgacgtat tttgcatgga ttacatattt
     9121 acgacataaa tgataaatgg agatttatat gaataactct cggttattcc gtttgagcag
     9181 gattgttatt gcgttaactg ctgccagcgg catgatggta aataccgctt acgcaacaga
     9241 tgaagcgaaa gccgccactc aatataccca acaggttaat cagaattacg ccaaatcatt
     9301 accgtttagc gatcgtcagg attttgacga cgctcagcgt ggatttatcg ctccgctgct
     9361 ggatgaaggt attctgcgcg atgctaatgg caaaccatat tatcgcgggg aagattataa
     9421 atttgatatc aatgcgcccg cgcccgaaac cgttaaccct agcctgtggc gtcagtcgca
     9481 gttaaacggt atttccggcc tgtttaaagt caccgacaga atgtaccagg ttcgcggtca
     9541 ggatatctcc aacatcacct ttatcgaagg tgataccggg attattgtca tcgacccgct
     9601 ggtgacgcca ccgagcgcaa aagccgccct tgacctttac ttccaaaatc gcccgcaaaa
     9661 accgattgtt gcggttattt atacccacag ccataccgac cactatggcg gcgtgaaagg
     9721 cattatctct gaagccgatg tgaaatccgg taaagtgcag gttatcgccc ctgctggctt
     9781 tatggacgaa gccatcagcg aaaacgtact ggcgggtaat attatgagcc gccgtgcact
     9841 ttactcctac ggcctgctgc tggcgcataa ccctcagggt aacatcggca atggtcttgg
     9901 cgtaacgctg gcatcaggct acccgagcat catcgcaccg aacaaaacca tcaccaaaac
     9961 cggtgagaag atgattatcg acggcctgga gtttgacttc ctgatgaccc caggtagcga
    10021 agcaccagcc gaaatgcact tctatattcc ggccctgaaa gcgctgtgta ccgccgagaa
    10081 cgccacgcat accctgcaca acttctacac tctgcgcggc gcgaaaaccc gcgacaccag
    10141 caagtggacc gagtatctga acgaaacgct ggatatgtgg ggtaacgacg cggaagtcct
    10201 gtttatgccg cacacctggc cggtctgggg caataagcat atcaatgatt atattggtaa
    10261 atatcgcgat actatcaagt acattcacga ccagaccctg cacctggcga accagggcta
    10321 caccatgaat gaaatcggcg acatgattaa actgccgcct gcacttgcca ataactgggc
    10381 cagccgtggc tattacggtt ctgtcagcca caacgcccgc gcggtgtata acttctatct
    10441 tggctattac gacggtaacc cggctaacct gcatccgtat ggtcaggtgg agatgggtaa
    10501 acgttacgtg caggcgctgg gcggttccgc acgcgttatt aacctggcgc aagaagcgaa
    10561 caagcaaggt gattaccgct ggtcggcaga actgctgaaa caggtgattg ccgccaaccc
    10621 gggtgaccag gtcgcgaaga atctgcaagc gaataacttt gaacagctgg gctatcaggc
    10681 cgagtccgcc acctggcgcg gtttctacct gaccggcgcg aaagagctgc gcgaaggggt
    10741 gcataagttc agccacggca ccaccggttc cccggacacc attcgcggga tgtcggtcga
    10801 aatgctgttc gactttatgt ccgttcgcct cgatagcgcg aaagccgcgg gtaaaaatat
    10861 cagcctgaac ttcaatatga gcaatggcga taacctcaac ctgacgctga acgatagcgt
    10921 gcttaactac cgtaaaacgc tgcaaccgca agctgacgcc tctttctaca tcagccgtga
    10981 agatctgcac gccgtgctga ccggacaagc caaaatggcg gatctggtaa aagcgaagaa
    11041 agccaaaatt attggcaatg gcgcgaaact ggaagaaatt atcgcctgtc tggataattt
    11101 cgatttgtgg gtgaatatcg taaccccaaa ttaatgtcac tcgcttttgg gggcgctgaa
    11161 gcccccaaaa gcatttattt taaaaatata tacagccaat taataaataa aatatataag
    11221 ttatgaatgc ggtggctgaa taaagctatt tttattcatc aagaaaatag ccatactgac
    11281 gccttcaatg gatgttgata ttcactacag atattgttcg cagcagacag gatgtattta
    11341 atatgccaac agttctctct cgcatggcga tgcaactcaa aaaaacagcc tggataattc
    11401 ccgtcttcat ggtttcggga tgctcattat ctccggcaat cccggtgatc ggcgcttatt
    11461 atcccagctg gtttttctgc gccattgcca gccttatttt gacgctcatc acgaggcgag
    11521 ttattcagcg ggcaaatatc aatctggcat ttgtcggaat tatttatacc gccctttttg
    11581 ctctctacgc catgctgttc tggctggcat ttttctaatc tatttgagac gcgctcatgg
    11641 aaagtacgcc gaaaaaagct cctcgcagta aattccctgc tctgttagtg gttgcgttgg
    11701 cgctggttgc ccttgttttc gttatctggc gcgtagacag tgcgccatca actaatgacg
    11761 cttacgcgtc agcagatacc attgatgtgg tgccggaagt cagcggtcgc attgtagaac
    11821 tggcggtcac cgacaaccag gcagtcaaac agggcgattt gctgttccgc attgacccgc
    11881 gcccgtacga agccaatctg gcgaaagctg aagcctccct cgcggcgctg gataagcaaa
    11941 ttatgctcac ccagcgtagc gttgacgcgc aacagtttgg tgccgactcg gttaacgcca
    12001 cggtagaaaa agcccgtgcc gccgcgaaac aggccacaga tacattacgc cgcaccgagc
    12061 cattactgaa agaaggtttt gtctcagcgg aagacgttga ccgtgcgagg accgcgcagc
    12121 gcgctgcaga agccgatctt aatgctgtat tgttgcaggc gcagtcagcc gccagcgccg
    12181 tcagcggcgt ggatgcatta gttgcccagc gtgcggcggt cgaagcggat attgccctga
    12241 ccaaactgca tctggaaatg gcgaccgttc gcgcgccgtt tgatggccgg gtcatttccc
    12301 tcaaaacctc cgtcgggcag tttgcatcta cgatgcgccc tatttttacc ctaatcgaca
    12361 ctcgtcactg gtacgtgatc gccaacttcc gcgaaaccga tctgaaaaat attcgctcag
    12421 gtacacccgc aacgattcgc ctgatgagtg acagcggcaa aaccttcgag ggtaaagtgg
    12481 attcgattgg ctacggcgtg ctaccggatg acggcggcct ggtgctgggc ggcctgccga
    12541 aagtttctcg ctctattaac tgggtccgcg ttgcccagcg ttttccggtc aaaatcatgg
    12601 tcgataaacc tgacccggaa atgttccgca tcggcgcttc ggcagtcgct aatcttgagc
    12661 cgcaataatg agcgcgctca actccctgcc attaccggtg gtcaggctgc tggcgttctt
    12721 tcatgaagag ttaagcgagc ggcgaccagg ccgcgtgccg cagaccgtgc aactctgggt
    12781 aggctgcctg ctggtgattc tgatctcgat gacctttgag atccctttcg tggcgttatc
    12841 gctggcagtg ctgttttacg gtattcagtc gaacgcgttt tacaccaaat ttgtcgcgat
    12901 cttatttgtg gtcgccacga tgctggagat cggcagcctg tttttgatct acaaatggtc
    12961 atacggcgaa ccgttgatcc gattgatcat cgccgggccg atcctgatgg gctgtatgtt
    13021 tttgatgcgc acccatcggc tgggactggt ctttttcgca gtcgccattg tcgccattta
    13081 cgggcaaacc ttccccgcca tgctcgacta tccggaagtg gtcgtgcgct taacgctgtg
    13141 gtgtatcgtt gttggcctct atccaacctt gctgatgacg ttaatcggcg tgctgtggtt
    13201 tcccagtcgt gccatttcgc aaatgcatca ggcgcttaat gatcggcttg atgatgccat
    13261 tagccacctg acggacagcc tcgcaccgct acccgaaacg cggattgaaa gagaggcgct
    13321 ggcgctgcaa aaactgaatg tcttttgtct cgcggacgat gccaactggc gaactcaaag
    13381 cgcatggtgg caaagctgcg tggcaacggt aacctacatt tactcgacgc tgaatcgcta
    13441 cgatcccacc tcttttgctg attctcaggc aattattgaa ttccgacaaa aattagcttc
    13501 agaaatcaac aagctgcagc atgccgttgc tgaaggtcag tgctggcaaa gcgactggcg
    13561 gatcagtgaa agtgaagcga tggcggcacg ggaatgtaac ctggagaata tctgccagac
    13621 gttgttacaa ctgggtcaga tggacccgaa tacgccgcca acgcccgcag ccaaaccgcc
    13681 atcaatggcc gccgatgctt ttaccaatcc agactatatg cgctacgcgg taaaaacgct
    13741 gctcgcctgt ttgatctgtt acacctttta cagcggcgtg gactgggaag gcattcacac
    13801 ctgtatgctg acatgcgtga tcgtcgctaa cccaaatgtc ggttcgtcgt accagaagat
    13861 ggtgctgcgt tttggcgggg ccttttgcgg cgcgattctg gcgctgttat tcacgctact
    13921 ggtcatgccc tggctggaca atattgtcga attgctgttt gtgctggccc cgattttcct
    13981 gttgggcgca tggattgcca ccagctctga acgctcttct tatatcggca cacagatggt
    14041 ggtcaccttc gcgctcgcca cgctcgaaaa cgtttttggc ccagtgtacg acctggtgga
    14101 aattcgcgat cgcgccctgg gtatcatcat tggtaccgtg gtgtccgcgg tgatttacac
    14161 ctttgtctgg cctgaaagtg aagcgcgcac gctgccgcaa aaactggctg gcgcgctggg
    14221 tatgttaagt aaagtaatgc ggatcccacg gcagcaggaa gtcacggctc tgcgcactta
    14281 tctgcaaatt cgtatcggtc tgcatgcggc gtttaatgcc tgtgaagaga tgtgccaacg
    14341 cgtggcgctg gagcgtcaac tggacagcga agaacgcgcc ttactgattg aacgttcgca
    14401 aacggttatt cgtcagggcc gcgatattct tcacgcctgg gatgccacct ggaactcggc
    14461 gcaggcgctg gataacgcac tacagccgga cagagcagct cagtttgccg acgccctgga
    14521 gaaatacgct gccggtctgg caaccgcact cagccgttct cctcaaataa cgcttgaaga
    14581 gacacccgcc tcgcaggcca tcctgcccac cttattaaaa caggagcaac acgtctgcca
    14641 gcttttcgcc cgcttgccag actggacagc cccggcatta acgcccgcca cggaacaggc
    14701 acaaggagcc acgcaatgat caatcgtcaa ctttcacgtc tgctgttgtg cagcattctc
    14761 ggcagcacga cgctgatttc cggctgtgcc ctggtacgta aggattctgc acctcatcaa
    14821 cagctcaaac cggaacaaat caaactggcc gacgatattc atcttgccag ctccggctgg
    14881 ccgcaggcgc agtggtggaa acaactcaat gacccgcagc tggatgcgct gatccaacgg
    14941 acgctaagtg gttcacacac cctcgccgaa gcgaaactgc gggaagaaaa agcgcagtcc
    15001 caggccgatt tgttagatgc cggttcacaa ttacaggtcg cagcgttagg gatgctcaac
    15061 cgccaacgcg tctcggcgaa cggcttttta agcccttatg cgatggatgc gcccgcactg
    15121 ggtatggacg ggccgtacta tacggaagcc acagtaggtt tgtttgccgg actggatctt
    15181 gatttgtggg gtgtgcatcg ctcagcggtt gccgccgcca ttggcgcgca taatgccgcg
    15241 ctggcagaaa ccgcagcagt agagctatcg ctgaccacgg gcgtagcgca gctttattac
    15301 agtatgcagg ccagctatca gatgctcgat ctgttagaac aaactcgcga tgtgattgat
    15361 tacgcggtga aagcgcacca gagtaaagtg gcgcacggtc tggaagcaca agtgcctttc
    15421 cacggcgcgc gggcacagat tctggcggtc gataaacaaa ttgctgccgt caaagggcaa
    15481 atcaccgaaa cgcgagaatc tctgcgtgca ttgattggcg cgggggccag cgatatgccg
    15541 gagatcaaac cggtggcatt accacgagtc cagaccggca ttccggcgac actctcttat
    15601 gagttgctcg ccagacgccc ggatctgcaa gccatgcgct ggtatgttca ggcgtcatta
    15661 gatcaggtgg attccgcgcg ggcgctgttc tacccgagct ttgatatcaa agcgtttttc
    15721 ggccttgatg ccatccacct ggatacctta ttcaaaaaaa ccagtcgcca gttcaacttc
    15781 atcccgggtc tgaaattgcc gctgtttgac ggtggacggt tgaatgccaa tctcgaaggc
    15841 acgcgcgccg ccagcaacat gatgattgaa cgttacaacc agtcagtact gaacgcggtg
    15901 cgtgacgttg ccgtcaacgg cacgcgtctg caaacgctca acgacgagcg agaaatgcag
    15961 gctgaacgcg tggaagcaac gcgcttcacc cagcgcgctg ccgaggccgc ctatcagcgc
    16021 ggcttaacca gccgcttaca ggccactgaa gcccggttgc cagtacttgc cgaagagatg
    16081 tcattactga tgctggacag ccgccgggtg atccaaagca ttcagttgat gaaatcgctg
    16141 ggcggcgggt atcaggcggc tcccgtcgtc gagaaaaaat aaaatgtctg ccgcgtgatg
    16201 gctgtcacgc ggtatttcgt ttcgtcacgt caaaactgac gacagcctgt ttttcgtcag
    16261 agttttgaat aaatagtgcc cgtaatatca gggaatgacc ccaaataaaa tgtggcataa
    16321 aagatgcata ctgtagtcga gagcgcgtat gcgtgatttg attaactgga gcgagaccga
    16381 tgaaaaaagt cgtcacggtt tgcccctatt gcgcatcagg ttgcaaaatc aacctggtcg
    16441 tcgataacgg caaaatcgtc cgggcggagg cagcgcaggg gaaaaccaac cagggtaccc
    16501 tgtgtctgaa gggttattat ggctgggatt tcattaacga tacccagatc ctgaccccgc
    16561 gcctgaaaac ccccatgatc cgtcgccagc gtggcggcaa actcgaacct gtttcctggg
    16621 atgaggcact gaattacgtt gccgagcgcc tgagcgccat caaagagaag tacggtccgg
    16681 atgccatcca gacgaccggc tcctcgcgtg gtacgggtaa cgaaaccaac tatgtaatgc
    16741 aaaaatttgc gcgcgccgtt attggtacca ataacgttga ctgctgcgct cgtgtctgac
    16801 acggcccatc ggttgcaggt ctgcaccaat cggtcggtaa tggcgcaatg agcaatgcta
    16861 ttaacgaaat tgataatacc gatttagtgt tcgttttcgg gtacaacccg gcggattccc
    16921 acccaatcgt ggcgaatcac gtaattaacg ctaaacgtaa cggggcgaaa attatcgtct
    16981 gcgatccgcg caaaattgaa accgcgcgca ttgctgacat gcacattgca ctgaaaaacg
    17041 gctcgaacat cgcgctgttg aatgcgatgg gccatgtcat tattgaagaa aatctgtacg
    17101 acaaagcgtt cgtcgcttcc cgtacagaag gctttgaaga gtatcgtaaa atcgttgaag
    17161 gctacacgcc ggagtcggtt gaagatatca ccggcgtcag cgccagtgag attcgtcagg
    17221 cggcacggat gtatgcccag gcgaaaagcg ccgccatcct gtggggcatg ggtgtaaccc
    17281 agttctacca gggcgtggaa accgtgcgtt ctctgaccag cctcgcgatg ctgaccggta
    17341 acctcggtaa gccgcatgcg ggtgtaaacc cggttcgtgg tcagaacaac gtgcagggtg
    17401 cctgcgatat gggcgcgctg ccggatacgt atccgggata ccagtacgtg aaagatccgg
    17461 ctaaccgcga gaaattcgcc aaagc
//
LOCUS       DAFATA010000094        17058 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFATA010000094 DAFATA010000000
VERSION     DAFATA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.095"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..173)
                     /locus_tag="LGJ20_003692"
     CDS             complement(<1..173)
                     /locus_tag="LGJ20_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HBK2387706.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSA"
     gene            complement(385..825)
                     /locus_tag="LGJ20_003693"
     CDS             complement(385..825)
                     /locus_tag="LGJ20_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBK2387707.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWLAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            926..1525
                     /locus_tag="LGJ20_003694"
     CDS             926..1525
                     /locus_tag="LGJ20_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBK2387708.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1633..2400
                     /gene="tpiA"
                     /locus_tag="LGJ20_003695"
     CDS             1633..2400
                     /gene="tpiA"
                     /locus_tag="LGJ20_003695"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK2387709.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2455..3210)
                     /gene="cdh"
                     /locus_tag="LGJ20_003696"
     CDS             complement(2455..3210)
                     /gene="cdh"
                     /locus_tag="LGJ20_003696"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBK2387710.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(3317..4306)
                     /gene="sbp"
                     /locus_tag="LGJ20_003697"
     CDS             complement(3317..4306)
                     /gene="sbp"
                     /locus_tag="LGJ20_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBK2387711.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4626..5588)
                     /gene="pfkA"
                     /locus_tag="LGJ20_003698"
     CDS             complement(4626..5588)
                     /gene="pfkA"
                     /locus_tag="LGJ20_003698"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK2387712.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5769..6671)
                     /gene="fieF"
                     /locus_tag="LGJ20_003699"
     CDS             complement(5769..6671)
                     /gene="fieF"
                     /locus_tag="LGJ20_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBK2387713.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6820..7320)
                     /gene="cpxP"
                     /locus_tag="LGJ20_003700"
     CDS             complement(6820..7320)
                     /gene="cpxP"
                     /locus_tag="LGJ20_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBK2387714.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7470..8168
                     /gene="cpxR"
                     /locus_tag="LGJ20_003701"
     CDS             7470..8168
                     /gene="cpxR"
                     /locus_tag="LGJ20_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBK2387715.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8165..9538
                     /gene="cpxA"
                     /locus_tag="LGJ20_003702"
     CDS             8165..9538
                     /gene="cpxA"
                     /locus_tag="LGJ20_003702"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBK2387716.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9644..10318)
                     /gene="yiiM"
                     /locus_tag="LGJ20_003703"
     CDS             complement(9644..10318)
                     /gene="yiiM"
                     /locus_tag="LGJ20_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBK2387717.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10467..11450)
                     /gene="kdgT"
                     /locus_tag="LGJ20_003704"
     CDS             complement(10467..11450)
                     /gene="kdgT"
                     /locus_tag="LGJ20_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBK2387718.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11710..12330)
                     /gene="sodA"
                     /locus_tag="LGJ20_003705"
     CDS             complement(11710..12330)
                     /gene="sodA"
                     /locus_tag="LGJ20_003705"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBK2387719.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12615..13649
                     /gene="rhaT"
                     /locus_tag="LGJ20_003706"
     CDS             12615..13649
                     /gene="rhaT"
                     /locus_tag="LGJ20_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBK2387720.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWTYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAINAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13646..14512)
                     /gene="rhaR"
                     /locus_tag="LGJ20_003707"
     CDS             complement(13646..14512)
                     /gene="rhaR"
                     /locus_tag="LGJ20_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBK2387721.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(14568..15404)
                     /gene="rhaS"
                     /locus_tag="LGJ20_003708"
     CDS             complement(14568..15404)
                     /gene="rhaS"
                     /locus_tag="LGJ20_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBK2387722.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15692..>17058
                     /gene="rhaB"
                     /locus_tag="LGJ20_003709"
     CDS             15692..>17058
                     /gene="rhaB"
                     /locus_tag="LGJ20_003709"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBK2387723.1"
                     /translation="MTFRNCVAVDLGASSGRVMLSRYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVST"
BASE COUNT         4270 a         4439 c         4235 g         4114 t
ORIGIN      
        1 tctgcagagg atgcagcggc ggcaagctct ttcagtttgg tcataaaaga ctctgcttct
       61 tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt tgtcgttatc aatcaccgta
      121 ccgatatcaa tcgcacatcc tttagtactg catttatcta cgacatcttt catagggcac
      181 ctctgtatat tcgcctctct gtcgattacg acttaagaat aatgcgccgt taagggaaga
      241 acaaacaagt aatgatctta tggattacca ttttttttga tattgcttaa ggtgaagagt
      301 tcagaaggat tttccgggca ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa
      361 tagcagtaag agatatatta aaggttaatt acttgattta ttgtcggctt tatacttcac
      421 atcctgggta tctttaccat atttattttc gccttgtgtg ccaatgaacg ctccaagatc
      481 gataagcatc atcaccagaa tcaacgtcgg gacaaaacgc cccaccgccc attgccagac
      541 acccggtaaa atcgcccagt tacccgccag cagcatccac gccacaatca tcagaaatgc
      601 ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaacg gttactgccg ctgttggcca
      661 aagcaagcac accaggcaaa acgccgcggt ctgaatatcg agtaaatttt tacccgccag
      721 tgagaaaagc accagcatgc ctgctaacca caggcctatc caaatccaga aatcacggcg
      781 tccaatacgc cctttaaatg agaataacca ttgctgtatg gtcatgtaag ttccttgatg
      841 gttgtctttt ccaggattct acccttttga caagggcgac agatatcgtt ttaatcggag
      901 ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg
      961 ttctgcatta accgttacaa caacggcgca tgcacaaaca ccagatacgg caacgaccgc
     1021 gccttatctg ctggctggag cccctacttt cgatctctcc atcagccagt ttcgagaaga
     1081 ctttaacagc cagaatccca gcctgccact gaacgaattt cgtgccatcg acagcagtcc
     1141 cgacaaagcc aatctcactc gtgctgcaag taaaattaac gagaacttgt atgcttctac
     1201 agcgctggag cgcggtacct taaaaatcaa aagcattcaa atgacctggc tacccatcca
     1261 ggggccagag caaaaagcag cgaaagcgaa agctcaggaa tacatggcag cagtgatccg
     1321 cacactcacc ccattaatga ccaaaacaca aagccagaaa aaactgcagt cgctactaac
     1381 ggcggggaaa aacaaacgtt attacaccga gacagaaggt gcactgcgtt atgttgtcgc
     1441 ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg attaagctgg cgctatctga
     1501 atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct ttgtgccgat gaatctctat
     1561 actgtttcac agacctgctg ccctgcgggg cggccatctt cctttattcg cttataagcg
     1621 tggagaatta aaatgcgaca tcctttagtg atgggtaact ggaaactgaa cggcagccgc
     1681 cacatggttc acgagctggt ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt
     1741 gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc
     1801 agccacatca tgctgggtgc gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt
     1861 gaaacttctg ccgctatgct gaaagacatc ggcgctcagt acatcatcat cggccactct
     1921 gaacgtcgta cttaccacaa agagtctgac gaactgatcg cgaaaaaatt cgcggtgctg
     1981 aaagagcagg gcctgactcc ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg
     2041 ggcaaaactg aagaagtttg cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct
     2101 gcggcattcg aaggtgcggt tatcgcttac gaacctgtat gggcaatcgg tactggcaaa
     2161 tctgcaactc cggctcaggc acaggctgtt cacaaattca tccgtgacca catcgctaaa
     2221 gttgacgcta acatcgctga acaagtgatc attcagtacg gcggctctgt aaacgcgtct
     2281 aacgctgcag aactgtttgc tcagccagat atcgacggcg cgctggttgg cggtgcttct
     2341 ctgaaagctg acgctttcgc agtgatcgtt aaagctgcag aagcggctaa acaggcttaa
     2401 gtctgacagg tgccggattt catatccggc acttactttc cttaactctt cgccttaacg
     2461 caaaatctca cactgatgat cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag
     2521 atttcgttgt gtcgccagca atacaaatga gttatcactc tgccgtacca tcgccagccc
     2581 gtagcttccc atatgttccc gcgcctcagg tacttcttct gccagcatca taaatgggct
     2641 gcgttgtacc agttcgcttt ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc
     2701 acctggcagt ggcaaccagc ggctgctgat gttcgccaga ttgttatcca gctgttcgcg
     2761 cacatcagga cgaatacaag agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg
     2821 ggagttgatc gccaaagaaa ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat
     2881 aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa
     2941 aggactttca gtaccgttaa tacgatacgt tggcatcaac agatattgca gtggaccatt
     3001 aagatctttt aaaaccacgt atccggcatt gggtttgact tccgcacatg gcgaaggatt
     3061 ttgattttgc tgctgattgg gcaaacattc ctcaaggaca atcttacgta atgtatccga
     3121 ctcttcaccg gttaatttcc agtaaccaat accggcagcc acaacggcga taactatcat
     3181 caccaaaaaa agaagacccg cttttttcat ctttttttcc ctgtacctca aagagatgca
     3241 agggtaacgc aaaatcgtga caaataaaaa acccggtcgc gagaaaccac cgggtttgat
     3301 aattatcctg gggaaatcag cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat
     3361 gctctttttg cgctttcgtc cagccgccga actcttcatc aatggtgaat aacttcagct
     3421 ttggaaacgc attttcgtac tttttcgcta cttcagcgtc gcgcggacgg tagtagtttt
     3481 tcgcggcaat ttcctgacct tctggcgagt agagatattt caggtaggct tccgccacct
     3541 ctttggtgcc ttttttctcg accactttat cgaccaccga cacggttggc tctgcgagga
     3601 tagactcact cggcgtgacg atttcgaatt tatctttccc cagttcattc gctgccagca
     3661 gagcttcgtt ttcccaggcg atcagtacat cgccaatccc gcgctcgaca aaggtgttag
     3721 tggagccgcg cgcgccagaa tccagaactt cgacgttttt atacagtgcc cgaacaaaat
     3781 cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag gcagccaggt
     3841 agttccagcg tgcgccacca gagcttttcg gattaggcgt gatcaccgaa acacccggtt
     3901 taatcagatc gttccagtca tggatctgct tcggattgcc cttacgcacc aggaaaacaa
     3961 tggtggaagt gtacggtgcg gagttatccg gcagacgttt gatccactct ttatcaatcc
     4021 gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc cagcgtgaca acatcagctt
     4081 caataccgtt gattaccgac gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca
     4141 ccacgttatc accagtttgc tgtttccagt gggcgctgaa tgccttgttg tactgttcgt
     4201 acaattcgcg cgttggatca tatgaaacgt taagaagctg aatatccttt gccataacgc
     4261 tggttgccgc cagcaaaaat gttaacccta cgccccactt gttcatcgcc cgactctctt
     4321 atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac caaacattaa agaataaaaa
     4381 aagattggct ataacttgcg ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc
     4441 tcatattgct gacaaagtgc gctttatcga tgccggatgc ggcgcgaacg ccttatccga
     4501 cctacaaaaa tttgtaaatt caataaattg cagaattcac gtaggcctga taagcgaagc
     4561 gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc ctttttccga
     4621 aatcattaat acagtttttt cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg
     4681 ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctggat acctacacaa
     4741 cgaccgccgt aacctgccag cagcagatcg atagcgtaag cgcccatacg ggaagccaga
     4801 atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg
     4861 gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt
     4921 tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt
     4981 accaggtctt cacggctgaa ttcaacttcc ggaactacaa cgaattcaca gccaccggca
     5041 atggccgcag ccagggtcag atcgccacaa taacgaccca tcacttccac cacggaaata
     5101 cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc
     5161 gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc
     5221 agaccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta ggaaccgtca
     5281 ccgccgataa ccaccagcgc gtcgatacca cgttttttca ggttttcgat agccacggcg
     5341 cggatgttct cgtcgcggaa ttccgggaag cgcgcagaac cgaggaacgt accgccacgg
     5401 ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc atacagaccc
     5461 agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga acgaacaacc
     5521 ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc
     5581 ttaatcatga ctacctctga actttggaat gcaaaataaa atctgttgcc ggaagtcttc
     5641 ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat
     5701 tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt
     5761 tatactgatt atgaaagcat agaccgttta ccctccctgg gtacgacgga acaggggtcc
     5821 tgatggataa ttacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc
     5881 gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata
     5941 aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag
     6001 gaagtcacga tatcaataat ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat
     6061 gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc
     6121 aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga
     6181 attgcgccgt tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg
     6241 ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc
     6301 gccacaattg tcacgataac cccgacgcct ggatctgtca tcggtgttgg agatatcaga
     6361 tgttgaatac ccgtcaaaaa caggaatagt gccgaaccgg agataaacat actttgcgcc
     6421 agcgccgcga gggactctgc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc
     6481 agggaatatc gcaccaccag taaattcgtc aacgacgcgc cgatatccac cagcgaatcc
     6541 accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc
     6601 aatagcaacg aagccatcgc cgtcgcagca atcgccgccc gactgaccag ccgtccataa
     6661 gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct
     6721 gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc atgtggggga
     6781 agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct
     6841 actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc
     6901 cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta acaggcgata
     6961 catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt
     7021 ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat
     7081 tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc
     7141 tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt
     7201 actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc
     7261 gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat
     7321 catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg
     7381 caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac
     7441 gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc
     7501 gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg
     7561 cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg
     7621 acgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc
     7681 agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg
     7741 agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta
     7801 ttcgcgcgat cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt
     7861 caccgacact ggaagttgat gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg
     7921 acgggcaaac gctggagtta accggtactg agtttaccct gctctatttg ctggcacagc
     7981 atctgggtca ggtggtttcc cgtgaacatt taagccagga agtgttgggc aaacgcctga
     8041 cgcctttcga ccgcgctatt gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc
     8101 gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg cggctatctg atggtttctg
     8161 cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct
     8221 ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct
     8281 ggatagcgaa cagcgtcagg ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa
     8341 cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc
     8401 accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg
     8461 cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca
     8521 gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga
     8581 taattaccaa ctttatctga ttcgtccggc cagcagttct caatccgatt tcattaactt
     8641 actgtttgac cgcccgctat tactgctgat tgtcaccatg ttggtcagta cgccgctgct
     8701 gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga
     8761 agttgcccag ggaaacttac gccagcaccc ggaactggaa gcggggccac aggaattcct
     8821 tgccgcaggt gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca
     8881 gcagcgtctg ctttctgata tctctcacga gctgcgcacc ccgctgacgc gtctgcaact
     8941 gggtacggcg ttactgcgcc gtcgtagtgg tgaaagcaag gaactggagc gtattgaaac
     9001 cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg gtgatgtccc gtaatcagca
     9061 aaaaaacgcg ctggttagcg aaaccatcaa agccaaccag ttgtggagtg aagtgctgga
     9121 taacgcggcg ttcgaagccg agcagatggg caagtcgttg acggttaact tcccgcctgg
     9181 gccgtggccg ctgtacggca atccgaacgc actggaaagt gcgctggaaa acattgttcg
     9241 taatgctctg cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg
     9301 tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat
     9361 tttccgtccg ttctatcgga ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt
     9421 ggggctggcg attgttgaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga
     9481 cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac
     9541 tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct
     9601 gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg gggttatttt ccccacaacc
     9661 ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc
     9721 ttttcgataa cccggcggcg gagagtaaac ggtgatattg atcgtcatca aacggcatat
     9781 gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca
     9841 gcggcgcgtc cgcagacact ttccctggcg ctatcacgct atacaaccac cccaccttac
     9901 ctgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc
     9961 aaggcgagcg cggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccca
    10021 tataaacatt actttccgtg agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa
    10081 caaacaactc cgcctgttcc ggaaattccc gcgcccagta gagataatgc tcacgaggat
    10141 aatgacacag cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt
    10201 ccagccccag ctccgtcagc atcaactcac catcaacctg gattttggca attgcgctgg
    10261 gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct
    10321 gttccccctc cacgagaaat ttccccatta gaccatattt attttatgtc aggcgtgctg
    10381 ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc
    10441 cggctgtggg gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct
    10501 gctctggctt tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc
    10561 acaatgaccg ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc
    10621 accatttctg caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca
    10681 attccggcag tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg
    10741 gtcacgataa ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca
    10801 gtcaaatcaa tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct
    10861 ttgctgaaaa attctcgtaa ttcagggtca aggttcccca gggcaaagcc caccaggaac
    10921 ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg caatccctgc agtgcccaga
    10981 ataatcatcg tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct
    11041 tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc cgccattggt catatccatc
    11101 gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc
    11161 ggaataatgc gcgaggcaat tgccgcaacc acccacgcga cggcaatttt tgttaccacc
    11221 agcgtaccgg atttacgcaa taccgttccc gtcgcgctta attttattga cgcccccatg
    11281 caaaaaaacc acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat
    11341 ccaaaatatt tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg
    11401 acgagcatca tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc
    11461 tgtcggcata tttttaaggg ctgatgcgtc attcctcaat ggagcatcag tgttgatgga
    11521 aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca attgaaacac
    11581 tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa aaaccaccgc
    11641 cgttggcgat ggttcatttt ttaagctgca atgcggccta tacgcctcat tgcagcaggc
    11701 ggcaaatgat tattttttcg ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt
    11761 ccagaactct ttaatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc
    11821 ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag
    11881 cggagaatcc tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca
    11941 tgcccagccg gaaccaaagc gggaagctgc cgctttttca aattctgctt tgaagttatc
    12001 aacggagccg aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt
    12061 tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac
    12121 ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt
    12181 ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg tttacgtagg tctgatggtg
    12241 tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc
    12301 atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat
    12361 tgttaatgcc gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt
    12421 atcaatgccg tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc
    12481 aaatcaccca cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag
    12541 gttaatcgac agcacgattt ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg
    12601 atgaggataa gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg
    12661 cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa
    12721 ccatgtggtc agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc
    12781 tgttactacc gaatttctgg acgtattaca gctcgtttag tctctctacg ctgctgcctg
    12841 tttttctgtt cggcgctatg tgggggatcg gtaatatcaa ctatggcctg accatgcgtt
    12901 atctcggcat gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc
    12961 tgatgacgcc aattatcaac ggcaatttcg atgtgctgat taacaccgaa ggcggacgca
    13021 tgacgttgct cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc
    13081 agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaagggctgg
    13141 tgctggcggt gatgtgcggc attttctctg ccgggatgtc ctttgcgata aacgccgcaa
    13201 aaccgatgca tgaagccgct gccgcactag gcgtcgatcc actgtatgtc gctctgccaa
    13261 gctatgtcat catcatgggc ggcggcgcga tcatcaacct cggtttctgt tttattcgtc
    13321 tggcaaaagt gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca
    13381 ctcacaatgt gttactctca gcactgggcg ggttgatgtg gtatctgcaa ttctttttct
    13441 atgcctgggg ccacgcccgc attccggcgc aatatgacta catcagttgg atgctgcata
    13501 tgagcttcta tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaatg
    13561 caggacgccg tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta
    13621 acatcgtcgg catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc
    13681 tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc
    13741 cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat
    13801 gacacactct gacctgtcgc agatattgat tgatggtcat tccagtctgc tggcgaaatt
    13861 gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg
    13921 gacttttcag gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg
    13981 ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga
    14041 acagcaactc agccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct
    14101 gaccgataac ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc
    14161 ctgcgctggc gctaaatccc ggaatcgccc cctgccagtc aagattcagc ttcagacgct
    14221 ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat
    14281 cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga tcgttgagta
    14341 catgcaggcc attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcag
    14401 caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa
    14461 aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa
    14521 gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaaa
    14581 ccatcccgtc cctgacgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc
    14641 gaaaagtggt tactgtcgct gaatccacag cgataggcga tgtcagtaac gctggcctca
    14701 ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc
    14761 gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag agaaaattga
    14821 tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc
    14881 aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag
    14941 agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct
    15001 tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg
    15061 cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg
    15121 agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta
    15181 tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta
    15241 tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga
    15301 aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac
    15361 gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt
    15421 cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa
    15481 aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga
    15541 gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt
    15601 gccaatggcc cattttcctg tcagtaacga gaaggtcacg aattcaggcg ctttttagac
    15661 tggtcgtaat gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga
    15721 tctcggcgca tccagtgggc gcgtgatgct gtcgcgttac gagcgtgaat gccgcagcct
    15781 gacgctgcgc gaaatccatc gttttaaaaa tgggctgcat agccagaacg gttatgtcac
    15841 ctggaatgtg gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga
    15901 agggattcgt atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga
    15961 ccaacagggt cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgct ccaatggcct
    16021 aatggcgcag gcacagcaac aactcggcaa acgcgatatt tatcaacgta gcggcatcca
    16081 gtttctgccc ttcaatacgc tttatcaact gcgtgcgctg acggagcaac aacctgaact
    16141 tattccacac attgctcacg ctctgctgat gccggattac ttcagctatc gcctgaccgg
    16201 caagatgaac tgggagtaca ccaatgccac caccacacaa ctggtcaata tcaatagcga
    16261 cgactgggac gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc
    16321 gacgcatccg ggtaatgtca ttgggcactg gatttgcccg cagggtaatg agattccggt
    16381 ggtcgccgtt gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc
    16441 acgcgccgct tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc
    16501 atttaccaat gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg
    16561 ctatcgggtg ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggaacg
    16621 gcaaatcaac gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgtcgctt
    16681 catcatcaat cccaatgacg atcgctttat taatcctgaa gcgatgtgca gcgaaattca
    16741 ggctgcgtgt cgggaaacgg cgcaaccgat cccggaaagt gatgctgaac tggcgcgctg
    16801 tattttcgac agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg
    16861 cggtgaagat ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa
    16921 ccagctatgc gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac
    16981 gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg tggatgattt
    17041 ccgtcaggtc gtcagcac
//
LOCUS       DAFATA010000095        17031 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFATA010000095 DAFATA010000000
VERSION     DAFATA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.096"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..668
                     /gene="rmuC"
                     /locus_tag="LGJ20_003710"
     CDS             <1..668
                     /gene="rmuC"
                     /locus_tag="LGJ20_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBK2387724.1"
                     /translation="IDAKMTLVAYERYFNAEDDYTRESALQEHIASVRNHIRLLGRKD
                     YQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITEALKNNIMLVSPTTLLVALRTIA
                     NLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAIGQSLDKAQDNYRQAMKKLSSGR
                     GNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEYRLRSVPEQPNDEAYQRDDEYNQ
                     QSR"
     gene            763..1518
                     /gene="ubiE"
                     /locus_tag="LGJ20_003711"
     CDS             763..1518
                     /gene="ubiE"
                     /locus_tag="LGJ20_003711"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBK2387725.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            1532..2137
                     /gene="ubiJ"
                     /locus_tag="LGJ20_003712"
     CDS             1532..2137
                     /gene="ubiJ"
                     /locus_tag="LGJ20_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBK2387726.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            2134..3774
                     /gene="ubiB"
                     /locus_tag="LGJ20_003713"
     CDS             2134..3774
                     /gene="ubiB"
                     /locus_tag="LGJ20_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBK2387727.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            3853..4122
                     /gene="tatA"
                     /locus_tag="LGJ20_003714"
     CDS             3853..4122
                     /gene="tatA"
                     /locus_tag="LGJ20_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBK2387728.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQANTNQEQAKTEDAKRHDKEQV"
     gene            4126..4641
                     /gene="tatB"
                     /locus_tag="LGJ20_003715"
     CDS             4126..4641
                     /gene="tatB"
                     /locus_tag="LGJ20_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBK2387729.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            4644..5420
                     /gene="tatC"
                     /locus_tag="LGJ20_003716"
     CDS             4644..5420
                     /gene="tatC"
                     /locus_tag="LGJ20_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBK2387730.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            5462..6244
                     /gene="tatD"
                     /locus_tag="LGJ20_003717"
     CDS             5462..6244
                     /gene="tatD"
                     /locus_tag="LGJ20_003717"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBK2387731.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(6241..6729)
                     /gene="rfaH"
                     /locus_tag="LGJ20_003718"
     CDS             complement(6241..6729)
                     /gene="rfaH"
                     /locus_tag="LGJ20_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBK2387732.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            6896..8389
                     /gene="ubiD"
                     /locus_tag="LGJ20_003719"
     CDS             6896..8389
                     /gene="ubiD"
                     /locus_tag="LGJ20_003719"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBK2387733.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            8435..9136
                     /gene="fre"
                     /locus_tag="LGJ20_003720"
     CDS             8435..9136
                     /gene="fre"
                     /locus_tag="LGJ20_003720"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBK2387734.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(9328..10491)
                     /gene="fadA"
                     /locus_tag="LGJ20_003721"
     CDS             complement(9328..10491)
                     /gene="fadA"
                     /locus_tag="LGJ20_003721"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBK2387735.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(10501..12690)
                     /gene="fadB"
                     /locus_tag="LGJ20_003722"
     CDS             complement(10501..12690)
                     /gene="fadB"
                     /locus_tag="LGJ20_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBK2387736.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            12880..14211
                     /gene="pepQ"
                     /locus_tag="LGJ20_003723"
     CDS             12880..14211
                     /gene="pepQ"
                     /locus_tag="LGJ20_003723"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBK2387737.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            14211..14825
                     /locus_tag="LGJ20_003724"
     CDS             14211..14825
                     /locus_tag="LGJ20_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBK2387738.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            14864..16315
                     /gene="trkH"
                     /locus_tag="LGJ20_003725"
     CDS             14864..16315
                     /gene="trkH"
                     /locus_tag="LGJ20_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBK2387739.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            16327..16872
                     /gene="hemG"
                     /locus_tag="LGJ20_003726"
     CDS             16327..16872
                     /gene="hemG"
                     /locus_tag="LGJ20_003726"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBK2387740.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDHCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         3911 a         4348 c         4691 g         4081 t
ORIGIN      
        1 tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta tttcaacgcc gaagatgact
       61 acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat atccgtttgc
      121 tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac gtgctgatgt
      181 ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag ctgatcaccg
      241 aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg gcgttgcgta
      301 ctattgccaa cctgtggcgt tatgagcatc aaagccgcaa cgcccagcaa atcgccgatc
      361 gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc gcgattggtc
      421 aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc tcttcaggtc
      481 gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa attaaacgcg
      541 agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat cgacttcggt
      601 cggttccgga gcagccgaat gatgaagctt atcaacgcga tgatgaatat aatcagcagt
      661 cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg acgcccaatc
      721 tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga taagtcacaa
      781 gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga tatggtcgcc
      841 cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat gtcatttggt
      901 attcatcgtt tgtggaagcg attcactatt gattgcagcg gcgtacgccg tgggcagacc
      961 gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg cctggtcgga
     1021 gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat gggacgcgag
     1081 aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa cgctgaggcg
     1141 ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct gcgtaacgtc
     1201 accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg cggccgcctg
     1261 ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta tgatgcatac
     1321 tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga cagctaccgt
     1381 tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc catgatgcag
     1441 gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt ggcgctgcat
     1501 cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag tgacggcagg
     1561 aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa cagcccgctc
     1621 gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt cattgattct
     1681 ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg ctgactgcac
     1741 cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc ttaccgcact
     1801 gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa acttcgttgc
     1861 gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata ccggtgatat
     1921 cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc atcacggcat
     1981 taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta tggcacccgg
     2041 tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg ctgttgatgc
     2101 cctgaccaaa cggctggaaa aactggaggc taaatgacgc caggtgaagt acggcgccta
     2161 tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc caaaatgcgt
     2221 atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa tcggcataaa
     2281 gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc ggtttggatc
     2341 aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat tgccgatcag
     2401 ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa gcagcagatt
     2461 gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga aatcaagccg
     2521 ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa tggtaaagag
     2581 gtggtgatta aagtcatccg cccggatatt ttgccggtta ttaaagcgga tctgaaactt
     2641 atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg tctgcgccca
     2701 accgaagtgg tgcgcgagta cgaaaagaca ttgattgatg aactgaattt gctgcgggaa
     2761 tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct ctacatcccg
     2821 gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat ttacggcatt
     2881 ccggtgtctg atgttgcggc gctggagaaa aacggcacca acatgaaatt gctggcggaa
     2941 cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca tgccgatatg
     3001 caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata tatcggcatt
     3061 gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc agaaaacttt
     3121 atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga ttctggctgg
     3181 gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt ctgtgaacct
     3241 atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa tctgtttaat
     3301 acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca gaaaaccctg
     3361 ctctacgtcg aaggggtagg acgccagctt tatccgcaac tcgatttatg gaaaacggcg
     3421 aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgcgct ggtgagagca
     3481 tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga actggtttac
     3541 gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc ccgcgagctt
     3601 cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg cgctacgtta
     3661 gtattaagtg gcacattctt gttggtcagc cgacctgaat gggggctgat gcccggctgg
     3721 ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg ctgatttttt
     3781 catcgctcaa ggcgggccgt gtaacgtata atgcggcctt gtttaatcat catctaccac
     3841 agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc cgtcatcgtt
     3901 gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg tgcgtcgatc
     3961 aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac cagtcaggat
     4021 gctgatttta ctgcgaaaac tatcgccgat aagcaggcga atacgaatca ggaacaggct
     4081 aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt tgatatcggt
     4141 tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc gcaacgactg
     4201 cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact ggcgacaacg
     4261 gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag tctgaaaaag
     4321 gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat ggatgaacta
     4381 cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga aaaggcgagc
     4441 gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc gcatgagggc
     4501 gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc agaaaccacg
     4561 ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc accttcccct
     4621 tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt atcacgcatc
     4681 taattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg atattcctgt
     4741 gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg atcaagcagt
     4801 tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt acgccgatca
     4861 agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat caggtgtggg
     4921 catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg ctgctggttt
     4981 ccagctcgct gctgttttat atcggcatgg cgttcgccta ctttgtggtc tttccgctgg
     5041 catttggctt ccttgccaat accgcgccgg aaggggtaca ggtatccacc gacatcgcca
     5101 gctatttaag cttcgttatg gcgttgttta tggcgtttgg tgtctccttt gaagtgcctg
     5161 tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta cgcaaaaaac
     5221 gcccgtatgt gctggttggt gcattcgttg tcgggatgct gctgacaccg ccggatgtct
     5281 tctcgcaaac gctgttggcg atcccgatgt actgtctgtt tgaaatcggt gtcttcttct
     5341 cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct gaagcagaaa
     5401 gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata tggagtacag
     5461 gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc gtgatgatgt
     5521 tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca ctaacctgcg
     5581 tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt caacggcggg
     5641 cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga ttattgagct
     5701 ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta accgcaactt
     5761 ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg ccgcagaatt
     5821 aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga cattgctgga
     5881 gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca cacgcgaaga
     5941 gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg tttgcgatga
     6001 acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga aattgctgat
     6061 cgaaactgat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat catcccggcg
     6121 caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc gtggagaaga
     6181 tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg ggattgcgtt
     6241 ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat taataagatt
     6301 aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct gaaagccttc
     6361 gaacgcgcct tcggtaataa tcaccttatc acccggataa ggggttgccg gatcgacaat
     6421 gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta tcgctggcga
     6481 cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg ttgatagtcg tggtatgaat
     6541 cacttctgga tcaaattcga caaacaggta gttgggaaac aatggctcac tgactgcagt
     6601 acgttttcca cgcacgattt tttccagggt gatcatcggt gccaggcaat tcacagcctg
     6661 tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca gtaaatacca
     6721 ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa agctttacgc
     6781 taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta atttaacaaa
     6841 tttacagcat cgcaaagatg aacgccgtat aatgggcgta gattaagagg ctacaatgga
     6901 cgccatgaaa tataacgatt tacgcgactt cttgacgctg cttgaacagc agggtgagct
     6961 aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg ctgaccgcac
     7021 tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact caatgccggt
     7081 gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc aggaagatgt
     7141 ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg agccgccaaa
     7201 aggtttccgc gacctgtttg ataaactgcc gcagtttaag caagtattga acatgccgac
     7261 aaagcggctg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg acgtcgatct
     7321 caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga ttacctgggg
     7381 gctgacagtg acgcgcggcc cgcataaaga gcggcagaat ctgggcattt atcgccagca
     7441 gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg gcgcgctgga
     7501 ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg tggcgctggg
     7561 tgccgatccc gccacgattc tcggtgcagt cacccccgtt ccggatacac tttcagagta
     7621 tgcgtttgcc ggattgctgc gcggtaccaa gaccgaagtg gtgaagtgta tctccaatga
     7681 ccttgaagtg cccgccagtg cggagattgt gctggaaggg tatatcgaac aaggtgaaac
     7741 ggcgccggaa gggccgtatg gcgaccacac cggttactat aacgaagtcg atagtttccc
     7801 ggtatttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt ccacctatac
     7861 cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag tgtttgtacc
     7921 gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg aaggctgttc
     7981 ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga agcgcgtcat
     8041 gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga tcgtttgcga
     8101 tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca cccgtatgga
     8161 cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg attttgcctc
     8221 gcctgtctcc gggctgggtt caaaaatggg gctggatgcc acgaataaat ggccggggga
     8281 aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtcg cgcatattga
     8341 cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat gcgcgtttgt
     8401 tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct gtaaagtgac
     8461 ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag acgcggcctt
     8521 ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca aacgtccgtt
     8581 ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg gcgcttctga
     8641 aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc aaatcgtggt
     8701 cgatattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga tgattttgat
     8761 tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt tggcgcgtaa
     8821 cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc tgtatgatct
     8881 ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc cggtggttga
     8941 acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg tattgcagga
     9001 tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga tggcgaaaat
     9061 tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt ttggcgatgc
     9121 gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag aacggcaact
     9181 aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaattggtg cacgacgcct
     9241 gatgcgacgc tggcgcgtct tatcaggcct acattggtgc cggatcggta gaccggataa
     9301 ggcgttcacg ccgcatccgg caagtggtta aacccgctca aacaccgtcg caataccctg
     9361 acccagaccg atacacatcg tcgccagacc aaactgaacg tctttgcgtt ccatcagatt
     9421 cagcagcgtg gtgctgatac gcgcaccgga acagcccagc ggatgaccca gcgcgatcgc
     9481 accaccattg aggttgatct tctcgtcaat ctgctccatt agcccgagat ctttaatgca
     9541 cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac acgccgatat cgctggcaga
     9601 aagccccgct tttttcagcg ccagcttcga ggccggaacc gggccgtaac ccataatcga
     9661 tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg cgcggcttaa gacctaattc
     9721 atgggcgcgg ctttcactca tcaccagcat ggcagctgcg ccatcggaaa gtgcagaaga
     9781 tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc ggacgcagcg tggcgagggc
     9841 ttccacggtg gtttccgggc gaatcacttc gtcgtaatta aactgcttca ggacgccgtc
     9901 ggcatcgtga ccaccagttg ggatgatttc atttttaaat gcggccgact gcgtggcggc
     9961 ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc atttcacggc tgataccgtg
    10021 catacgcgcc agcatttctg ccgttaagcc catcatgccc gccgctttgg cgacattgcg
    10081 gctcaggccg gggtgaaaat cgacgccgtg actcatcggc acatggccca tatgctccac
    10141 gccgccaacc agacatgcct gcgcatcgcc agtcatgatc attcgtgctg cgtcatgcag
    10201 tgcctgcatg gatgaaccac acaagcgatt gacggtaacc gccgggacag agtgcggcac
    10261 ttctgccagc agcgccgcgt tacgggcgat attaaaaccc tgctccagcg tctgctgcac
    10321 acaaccccag taaatatcgt cgagggccgc cgcttccagc gccgggttac gcgccagcag
    10381 gctacgcatt aaatgagcgg agagatcttc tgcacgcacg ttacgaaaag caccgccctt
    10441 cgaacggccc atcggggtgc gaattgcatc gacaatgaca acctgttcca ttgtgactcc
    10501 ttaagccgtt ttcaggtcgc caaccggacg ggctggctca accgggggat agtacggttc
    10561 gttatgacgc gctttattac gcagaccttc cggcacttca tacagcgggc cgaggtgctg
    10621 atattgctgt gccatatcga ggtattttgc gctaccgagg gtgtccagcc agcggaacgc
    10681 gccgccgtgg aacggaggga agcccaggcc gtagaccagc gccatatccg cttccgccgg
    10741 agtggcgata atgccttcct ccagacagcg caccacttcg ttgaccatcg ggatcatcat
    10801 gcgggcgata atctcttctt cgctgaaatc gcgcttcggc tggctcactt ctgccagcag
    10861 gtcttcaacg gcggcgtctt cttctttctt cggcttacct ttgctgtctt ctttataacg
    10921 ccagaaaccg aggccgttct tctgaccaaa gcggttggca tcaaacagcg cgtcgatggc
    10981 atcgcggtaa tctttctgca tccgctgcgg gaagcctgct gccatgacag cctgagcgtg
    11041 atgcgcggta tcaatgccca cgacatccag cagatatgcc ggacccatcg gccagccaaa
    11101 ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg ccgtcgcgca gcagctggct
    11161 gaaaccggcg aaatacggga acagcacgcg gttaacaaag aagccggggc agtcgttaac
    11221 cacaatcggc gtcttgccca tcttgctcgc ccaggcgaca actttcgcga tggtttcgtc
    11281 ggagcttttc tcgccgcgaa taatttctac caacggcatc cggtgtaccg ggttaaaaaa
    11341 gtgcattccg cagaagtttt ccgggcgttc cagcgcgttg gccagttcgc tgataggaat
    11401 ggttgaagtg ttagacgcca gcacggtatc cgggcgtact ttttgttcgg tttctgccag
    11461 tacggctttt ttcactttcg ggttttcgac aaccgcttct accacaacat ccacgcggtc
    11521 aaacccggcg tagtcgagcg ttgggtggat tgtggagatc acgccagcca gtttcagacc
    11581 atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc gcggcttcgg tcatgccgag
    11641 ggttaacgac ttgtcgttga tatctttcat gacaaccggc acgcctttcc acgcagactg
    11701 gtaagcgatg ccgccgccca taatgcctgc acccagcacc gcggcctgtt tcggggtttc
    11761 aacgtctttg gtgagtttct tcgctttgcc ttttacatat tgatcgttaa ggaaaatgcc
    11821 gaccagtgcg cgggcttcgt ttgtatgcgc cagcgggaca aaacttttgt tttccaggtt
    11881 taaggcttct tcacgaccaa aacgggccgc agcttcaatg gtttttactg cggtgatggg
    11941 ggccggataa tgtttccccg ctgtttgtgc gaccatccct ttagcgatgg tgaagctcat
    12001 ggtggcttca atcttgctca gttttagtgg ttccagcttc ggctgacgtt ttgctttcca
    12061 gtcgaggtcg ccgttaatgg cctggcgtaa aaccgccttt gcgccttcaa ccagtttttc
    12121 tgctttgact acgccatcca ccagaccgat tttcagcgcc tgatccgcgc cgacatcttt
    12181 accggcggca atgatttcca gcgcactgtc agcgcccagc atacgtggca tacgtacaga
    12241 accgccaaag cccggcatga tgcccagttt ggtttccggc agaccgatgc gcagatccgg
    12301 cgtcgccaga cgataatcgg tcgccagcac gcattcgcag ccaccgccca gcgcatagcc
    12361 gttgacggca gcaatggtcg gcaccggcag atcttccagg cgattaaaca cgctattggc
    12421 aaagtgcagc cactgactta actgttcttc aggaacgagg aacagggaca aaaattcggt
    12481 gatatcagca ccgacgataa aggctgcttt gttcgaacgc agcagcagcc cttttagatc
    12541 tgattgctgt tccagcacgc cgatggcctc gccgaggctg gcgacggtcg cagtgtcgag
    12601 tttattaact gaacctgggg catcaaatac cagttcggca atgccatctt ccagccagtc
    12661 aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca aggtgatctg
    12721 gtcgtaccag atgcatcgaa gtgtgtattt tgtgttaaaa atatgcaaat aaaagattaa
    12781 agaaatgccg atctgatcac gctcggcaga aatcacgctc tggatgaacg atgtgctaag
    12841 atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact ggcctcactc
    12901 tataaaaatc atatagctac cttacaggaa cggactcgcg atgcgctggc gcgcttcaag
    12961 ctggatgcgt tacttattca ctccggcgaa ctgttcaacg tttttctcga cgatcatccc
    13021 tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca ggtgccaaac
    13081 tgctggctgc tggtggatgg cgtgaacaag ccgaaactgt ggttctatct gccggttgat
    13141 tactggcaca acgtcgaacc gctgccgacc tctttctgga ctgaagacgt ggaagtgatc
    13201 gcgctgccga aagccgatgg cattggtagc ctgctgcctg ctgcgcgcgg gaatatcggt
    13261 tatatcggtc cggtgccgga acgtgcgctg caactgggta ttgaggccag caatatcaac
    13321 ccgaaagggg tgatcgacta cctgcattac taccgctcct tcaaaaccga gtacgaactg
    13381 gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc atcgcgcggc agaagaagcg
    13441 ttccgttctg gcatgagcga gttcgatatc aacattgcct atctgaccgc gaccggtcat
    13501 cgtgataccg acgtacctta cagcaacatt gtggcgctca acgaacacgc tgcggtgttg
    13561 cattacacca aactggacca ccaggcaccg gaagagatgc gcagcttcct gctggatgcc
    13621 ggggccgaat ataacggcta tgcggctgac ctgacccgta cctggtcggc aaaaagtgac
    13681 aacgactacg cacagctggt gaaagacgta aatgatgaac aactggcgct gatcgctacc
    13741 atgaaagcag gcgtcagcta tgtggattac cacatccagt tccatcagcg catcgccaaa
    13801 ctgctgcgta aacatcaaat catcaccgat atgagtgaag aagcgatggt cgaaaacgat
    13861 ctcaccggac cgtttatgcc gcacggtatt ggccatccgc tgggcctgca ggtgcatgac
    13921 gtagccggtt ttatgcagga tgatagcggt acgcacctcg cggcaccggc aaaatatccg
    13981 tacctgcgct gcacccgtat tctccagccg ggcatggtgt taaccatcga accgggtatc
    14041 tacttcattg aatcgctgct ggcaccgtgg cgtgaagggc agttcagcaa gcacttcaac
    14101 tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc gtatcgaaga caacgtggtg
    14161 atccacgaaa acaacgtgga aaacatgacc cgggatctga aactggcgtg atggaaagct
    14221 ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat caaaaagagc cgcttcataa
    14281 cgatgttggc gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa tcggtgcggg
    14341 cagaacaccc cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg ccggatgatt
    14401 ctcaacagct gggtttctct gacgacgggg agccggcggg aacggcaggt aaaccgatgc
    14461 tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc tgtggtagtg cgctactacg
    14521 gcggcatatt gcttggcacc ggtgggttag tgaaagcgta tggcggcggc gtgaatcagg
    14581 cgctgcgcca gctaacgacc caacgcaaga cgccattaac cgaatatact ttgcaatgtg
    14641 aatatagtca gttaaccggc attgaagcgt tgctggggca gtgtgacggc aaaattatca
    14701 acagtgatta tcaggcattc gttctgctgc gggtggcgct tccggcggcg aaagtggctg
    14761 aattttccgc aaagctggcg gattttagcc gtggttcatt gcaattgtta gcgattgaag
    14821 aataatcccc acttcgtttt gcagaactaa ggaagcggca gagatgcatt ttcgcgccat
    14881 tacccgaatc gttggactac tggtcatctt attttcaggg accatgatta tccctgggct
    14941 ggtagcactc atctaccggg atggagcggg ccgcgctttt acccagacct tttttgtcgc
    15001 cctcgccatt ggctctatgc tgtggtggcc gaaccgcaaa gagaaaggcg aacttaaatc
    15061 ccgtgagggg tttctgatag tggtgctgtt ctggaccgtg ctgggtagcg tcggtgcgct
    15121 cccttttatc ttctcggaaa gcccgaacct cacgattacc gatgcgtttt ttgaatcttt
    15181 ctctggcctg accaccaccg gggccactac gctggtgggg ctggattcgc tccctcacgc
    15241 catcctcttt tatcgccaga tgctgcaatg gtttggcggg atggggatca tcgtgttagc
    15301 ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg cagaaatgcc
    15361 cggcccgctg aaagataaca aaatgcgccc gcgaattgcg gaaacggcga aaaccctgtg
    15421 gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg ccggaatgga
    15481 tgcctttgat gccatcggcc atagctttgc gactatcgct attggcggct tctcgacaca
    15541 tgatgccagt atcggttatt tcgatagccc gactattaac actatcattg ctatcttcct
    15601 gctgatctcc ggctgtaact acggtctgca cttttcactg ttaagtgggc gtagtctgaa
    15661 ggtttattgg cgcgatccgg aatttcgcat gtttatcggc gtacagttta cgctggtggt
    15721 tatttgtacc ctcgtactgt ggtttcataa tgtctacagt tcggcgctga tgacaattaa
    15781 ccaggcgttt ttccaggtgg tgtcgatggc gacaaccgcc gggtttacga ctgacagcat
    15841 tgcccgctgg ccgctctttt tgccggtact gcttttatgt tcggctttta tcggcggttg
    15901 cgccgggtca acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt ttaagcaggg
    15961 gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta agctggggaa
    16021 tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga tttttctccg cctatgcatt
    16081 ggtgtttatt gtcagtatgc tggcgattat cgccacgggc gtggatgact tttctgcctt
    16141 tgcctcagtt gttgcgacat tgaataacct ggggccaggg cttggcgtgg ttgctgataa
    16201 ctttaccagt atgaacccgg tggctaaatg gatcctgatt gccaacatgc tgtttggtcg
    16261 tctcgaggtc tttacattgc tggtgctctt taccccgact ttctggcgtg aatgatggag
    16321 taatacgtga aaacattaat tcttttctca acaagggacg gacaaacgcg cgagattgcc
    16381 tcctacctgg cttcggaact gaaagaactg gggatccaga cggatgtcgc caatgtgcac
    16441 cgcattgaag aaccacaatg ggaaaattat gaccgtgtgg tcattggtgc ttctattcgc
    16501 tatggtcact accactctgc gttccaggaa tttgtcaaaa aacatgcgac gcgactgaat
    16561 tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga gaagcgtact
    16621 ccacagacca acagctacgc gcggaagttt ctgatgaact cgcaatggcg tcccgatcac
    16681 tgcgcggtca ttgccggggc gctgcgttac ccacgttatc gctggtacga tcgttttatg
    16741 atcaagctaa ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga agttgtctat
    16801 accgattggg agcaggtggc gaatttcgcc cgagaaatcg cccatttaac cgacaaaccg
    16861 acgctgaaat aagcataaag aataaaaaat gcgcggtcag aaaattattt taaatttcct
    16921 cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac
    16981 acgccgccgg gtcagcgggg ttctcctgag aatctcaaca gagaaaagta a
//
LOCUS       DAFATA010000096        16859 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFATA010000096 DAFATA010000000
VERSION     DAFATA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.097"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..982
                     /gene="yhjD"
                     /locus_tag="LGJ20_003727"
     CDS             <1..982
                     /gene="yhjD"
                     /locus_tag="LGJ20_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBK2387741.1"
                     /translation="QDLEHEPIKQLDNSEKGGKVSQALETVTTTAEKVQRQPVIAHLI
                     RATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPMLLQDIFDKILQN
                     ISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAIRAQSRDVWERS
                     PQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALHLNSIEWLKPTW
                     RLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVIKIVMTYTLPSL
                     MKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGKTQP"
     gene            1393..2715
                     /locus_tag="LGJ20_003728"
     CDS             1393..2715
                     /locus_tag="LGJ20_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBK2387742.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(2949..5009)
                     /locus_tag="LGJ20_003729"
     CDS             complement(2949..5009)
                     /locus_tag="LGJ20_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBK2387743.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(5079..5846)
                     /gene="pdeH"
                     /locus_tag="LGJ20_003730"
     CDS             complement(5079..5846)
                     /gene="pdeH"
                     /locus_tag="LGJ20_003730"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBK2387744.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            6078..7007
                     /gene="kdgK"
                     /locus_tag="LGJ20_003731"
     CDS             6078..7007
                     /gene="kdgK"
                     /locus_tag="LGJ20_003731"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBK2387745.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(7103..8599)
                     /locus_tag="LGJ20_003732"
     CDS             complement(7103..8599)
                     /locus_tag="LGJ20_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK2387746.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(8820..10106)
                     /gene="dctA"
                     /locus_tag="LGJ20_003733"
     CDS             complement(8820..10106)
                     /gene="dctA"
                     /locus_tag="LGJ20_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBK2387747.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(10289..12277)
                     /gene="hmsP"
                     /locus_tag="LGJ20_003734"
     CDS             complement(10289..12277)
                     /gene="hmsP"
                     /locus_tag="LGJ20_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBK2387748.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSALSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(12359..15832)
                     /gene="bcsC"
                     /locus_tag="LGJ20_003735"
     CDS             complement(12359..15832)
                     /gene="bcsC"
                     /locus_tag="LGJ20_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBK2387749.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDHDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(15814..>16859)
                     /gene="bcsZ"
                     /locus_tag="LGJ20_003736"
     CDS             complement(15814..>16859)
                     /gene="bcsZ"
                     /locus_tag="LGJ20_003736"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBK2387750.1"
                     /translation="ACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFFAL
                     AANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAW
                     SLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSMLLPGKVGFAEDNSWRFNPS
                     YLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLI
                     SSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGP
                     VGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNYVLTLFGQGWDQHRFRFSTKGE
                     LLPDWGQECANSH"
BASE COUNT         3804 a         4753 c         4321 g         3981 t
ORIGIN      
        1 ccaggatctg gagcacgagc cgattaagca gttggataat agcgagaaag gcggcaaagt
       61 tagccaggcg ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc aaccggttat
      121 tgcgcacctg attcgcgcga cagaacgctt taacgatcgg ctgggtaacc agtttggtgc
      181 ggctatcacc tatttctcat ttttatcgat gatcccgatt ttgatggtgt cgtttgccgc
      241 agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg acaaaattct
      301 gcaaaacatc agcgatccga cgctagccgc cacgttgaaa aacaccatca acaccgccgt
      361 tcagcagcgt acgactgtag ggcttgtcgg cctggcggtg gcgctttatt ccggcatcaa
      421 ctggatgggt aacctgcgtg aagcgattcg tgcccagtcg cgcgatgtct gggaacgctc
      481 gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt cgctgattgg
      541 tttgttgatt gcgctgattg tgacgctttc gatcacctcg gttgccggtt cggcgcagca
      601 aatgattatt agcgccctgc acctgaacag cattgagtgg ctgaaaccga cgtggcggtt
      661 gattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttctgga tcttctggcg
      721 gttaccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc tggctgctat
      781 tggttttgaa gtgattaaaa tcgtgatgac ctacaccctg ccatcgctga tgaaatcccc
      841 ctctggcgca gcatttggtt ccgtgctggg gctgatggca tttttctact tcttcgcccg
      901 tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg acccgcgaat
      961 gccagggaaa acgcagcctt aaaataggcc ggatgcggcg taaacgcctt atccggcata
     1021 cgattttatt cggcctggct ccccgtaggc cggataagat gcgccagcat cgcatccggc
     1081 tataatgcgc acataacctc ttgaaactct tccccagagc ctcttcagcc atctatttgg
     1141 gagcaaacaa tttcattcca actcataacc ccagcatata aatccagttg gtaactttta
     1201 tttaacctga aaccagtttt atccactatt tataaaatta tgtgaagcat ttcatagaag
     1261 aaaaatcact ggcctaaaca ttatcccctt tttgcctggt ttttgaccat ttccgcgatt
     1321 tgttacacat tgaaatatca cttttgctgt gcgtaatatg gctattcgtt agccaaaaaa
     1381 taagaaaaga ttatgcaagc aacagccaca acactcgacc acgagcaaga atacacgcca
     1441 atcaactcgc gtaataaagt ccttgtcgcc tctctcattg gcacggccat tgagttcttc
     1501 gacttttaca tttacgccac tgcggccgtt attgtgtttc cgcatatctt cttcccgcag
     1561 ggcgatccaa cggcagcaac gctacagtcg ctcgccacct tcgccatcgc cttcgtcgcg
     1621 cgccccattg gctctgccgt ttttggtcat tttggcgatc gcgttgggcg taaagcgacg
     1681 ctggtcgcct cgttgctaac gatggggatt tcgaccgtgg tgattggtct gctgccgggc
     1741 tatgccacga ttggtatttt cgccccgctg ctgctggcgc tggctcgatt tggtcagggt
     1801 ctgggcttag gcggtgaatg gggcggcgcg gcgctgctgg cgactgaaaa cgccccaccg
     1861 cgcaaacgtg cactgtatgg ctccttccca cagctgggcg caccgattgg cttcttcttt
     1921 gccaacggca ctttcttgct gctttcctgg ctactgaccg acgagcagtt tatgagctgg
     1981 ggctggcgtg tgccatttat cttctcggcg gtgctggtca ttatcggcct gtatgttcgc
     2041 gtgtcgctgc atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa acaggtgaag
     2101 atcccgctgg gtacgctgct gaccaaacat gttcgcgtaa ccgtactggg tacgttcatc
     2161 atgctggcaa catatacgct gttttacatc atgacggtct actctatgac cttcagtacc
     2221 gccgccgcgc cagttgggct tggcctgcca cgtaacgaag tgttgtggat gctgatgatg
     2281 gcagttattg gttttggcgt gatggtgcca gtcgccggat tactggctga tgcctttggt
     2341 cgccgtaaaa gcatggtaat tatcaccacg ctgatcatcc tgttcgcgct gttcgccttt
     2401 aacccgctgc tcggttctgg caacccgatt ctggtttttg ccttcctgct gctggggtta
     2461 agtctgatgg ggctgacctt cgggccgatg ggcgcgctgt taccagagct gttcccaaca
     2521 gaagtgcgtt acaccggagc atcgttctct tacaacgtag cgtcgattct cggggcttcc
     2581 gttgcgccgt atatcgcagc ctggttgcag actaactacg ggttaggtgc ggtggggtta
     2641 tatctggcgg cgatggctgg cttgacgtta atcgccctgc tgctgaccca tgagacgcga
     2701 catcagtcgt tgtaagtatc gtgtgggcag ccaccctcac cccagccctc tccctgaaag
     2761 ggagagggag aaaagcggag cgagatgtta tatcgcgaac ataacagccc tcccccagaa
     2821 agtgaacgga aaagcggtac tcgttgtttc aacacgcaca aaacagtccc ctcgcccctt
     2881 tggggagagg gttagggtga ggggaacacg ctgctcccga actcaccagc aaacacaatc
     2941 caacctcatc acttcttcat ctgcgacaat atcgtccgac actgattagc ctccccttcg
     3001 gaaggtgaga tcagtgccag taacgccgcc gccggtgtta ccagcgttgc cagtgccgca
     3061 gcaacagcac cacgggcaat caacggcccg gctttcaccc cagcctgcgg atttttaaac
     3121 gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaatcccttt actctccgga
     3181 tcaatagtca aatccagctg ttccgaagca aaacttgccg tgccggtaac attaatcaac
     3241 gcgttctcag tatcgaaagc aaaaatctgc gggcgcgcca cgccgttggc aatattcaga
     3301 ttcgccgccg cgcagttcac ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag
     3361 ttgccgacat tcagcccaac aatctccatc aggttgcggc tcaccagccc gtcattcatc
     3421 aacagtttca ggttgccgtt actgttgcct aaaagcgccg ccaccgagtt accgctaccg
     3481 cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc cacatcgggc
     3541 atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccctg catcggcttt
     3601 ttatcgcctt ccagatggat attcgccgca atgctgccgc ccgccatgcc aaatttcagc
     3661 ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc gctaatcggc
     3721 aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc cataacgtcc
     3781 catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg aacgcttttt
     3841 tcgcccttct tctgttcaga ccgtttcgac ttttctgccc ctttcccgga gtcaacgcca
     3901 attaacggtc ccaggtccgc cagccgcaat tgccgcgact cgacatcacc ttccagtttt
     3961 ggtcgtggct ttccggtggt gtagaccaga gaaccgtgga tatcgctatc gccaattcgc
     4021 ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc
     4081 cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga
     4141 tcacccagtg aatcgccaga aaatttaagc cgtaaatcga caccgcccat cttcattggg
     4201 tcattcacga cgccatcaaa agcaacacgg gtgttaccag agcggaaatc agcctgtacc
     4261 ggaaacggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtacccgtg
     4321 agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt
     4381 tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg cttacctaac
     4441 ggatcaacaa aaatctccag atccgctttg cttactttgt catcaatggc gatccgccct
     4501 tgatcgaaaa gaatattatc cagccgaaac gaccatgccg acggctttgc attcgcgtct
     4561 ttgttatcat cgttggcgag attaaacgtc cagttattgt tcttttcgga gaggcgaatc
     4621 aggcgcgcgt cgggcttttc gagcttgatc cacggcagcc agaccgtttt ggtcagcagc
     4681 gccagcgggg ccagcgttgc ctctacgcgt ggcaaatgca ccatcgtgac ttcgggaata
     4741 tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg cacccagctg
     4801 cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg
     4861 aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag
     4921 tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc cactgaaatc
     4981 gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag
     5041 tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag aaccgccgta
     5101 ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc
     5161 gagttctgaa catcacgcca ctcttccggc gtttctacgc cctcgacaat caccccgcga
     5221 caatagcgat tcattagatg taaaagctga gaaaagagtg tgcgtccttc cggcgactga
     5281 cgcagcatca caaacagttc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagt
     5341 gcagagaaat ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc aaattcacac
     5401 atcgaggcaa aggttgaatc tttcggcaga cggatatgct ccaccagttc gaaacgcagc
     5461 cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta
     5521 gggccatcaa tattgaccga tgccagcagg ccgtgctcta taaagaagtc ggctttttgc
     5581 gccagcaaat caatctgctc tttcacaacc tccatccgat ggctgacggt gatttcagta
     5641 aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac caccgttaat
     5701 agctccacgg ccattaaccg tccgcatgtt tgatagatcg gctgccaggt gtaagcacgc
     5761 tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt
     5821 attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc
     5881 ggactcgaca aagattatcg gcgcgtaagg gcagaacttt accgcctggc cggggtaaat
     5941 ttcctcactc cacaacggaa tctttcagga atacgtcctg gctcaaaaaa ataatggaac
     6001 actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac
     6061 agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc
     6121 tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg
     6181 tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta acggcgctgg
     6241 gaacggacag ttttagccag caaatgctgg acgcctggca cggcgagaac gttgatactt
     6301 ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca
     6361 ccggtgaacg tacgttctac tactggcgta acgaagccgc tgccaaattc tggctggaga
     6421 gcgagcagtc tgcggcgatt tgcgaagagc tggcgaattt cgattatctc tacctgagcg
     6481 ggattagcct ggcgatctta agcccgacca gccgcgaaaa gctgctttcc ctgctgcgcg
     6541 aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg
     6601 ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg gatatcgcct
     6661 tcctgacgct ggacgacgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg
     6721 cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc
     6781 tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtgaaactg ccgaaagaaa
     6841 aagtgatcga taccaccgca gctggcgact ctttcagtgc cggttatctg gcggtacgtt
     6901 taacgggcgg cagtgcggaa aacgcggcga aacgcgggca cctgaccgca agtaccgtta
     6961 ttcagtatcg cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaaag cagcatatgc
     7021 aaaaggctgg ataaagtgtt acccctctat ccagctcatc cataaatacc tgatgattgt
     7081 gctaactcat caccatcagg cattactgtg caggtggaat atccgtcact tcaggatgta
     7141 cgtcatccgt ggcgacagag gtggtcgcag cggcggtaga tggggccatg atttgatccc
     7201 aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc agcagtatta
     7261 acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc accgtcaggc
     7321 tgttaaggaa atcctgccgc agtttctgat actgctccgg cgcgatatcg acgacctgat
     7381 tttgcaacga acgcatccgc tgacccatca gaatatcggt atcagcccgc gcataggcgg
     7441 caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac tcttcttccg
     7501 gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg ttgctgttca
     7561 gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac agcacacggc
     7621 agtcaaatcc aagaccgatg tctttgctgt tactggcgct taacgcttgc tgaacatgcc
     7681 agaacagcgc ctcgcgggcc aggtccgcac gccagtagcg cagcagtgcg gctgattcac
     7741 gaatcggctg ccacggcgta tcccacatga tagataaccg gtcctgacgc accgcgtcag
     7801 tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga gctggcgttt
     7861 cacgtttgcc tttcagttcg ccaaacgttt tgttgatttg gtcgacaacc gagcgcgcat
     7921 ccacgtttcc caccaccagt agcgtcattg catccggggt gtaccatttc tgatagaaat
     7981 ctttaatttt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg tgacctaaca
     8041 aggttgatcc tttcagacga taacgccacc agccctcttt agtatcggca ggccaggttg
     8101 ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggtgtgata gtcagtttgc
     8161 cagtggcatt tgccaggtaa gagagcgcct ctttcagcaa atcgttacgg ttattgggca
     8221 aactcagatt aaacagcgta gtgtcatacg agacaattac cggcggcatc gggcgtttag
     8281 ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca ccgctttgcg
     8341 ttagcgcaat acgagggatg gcgtgactgt aaccgctctg ttgtgtactt tcggcgagcg
     8401 aaccggtatt aaccagcagg cgaatttcaa cacgatcgct gggacgctgg ggggtggtca
     8461 gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga tcaggctgga
     8521 gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc
     8581 gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa aaaaactgcc
     8641 cgttgtcggt cagtctaaat gacgttaaaa acacttcgtg ttagaccgca agaacatgaa
     8701 aatgtcacgg aagaagtgaa ataaacctga actcacccag gtggtaagtt caggcacagg
     8761 ggtcaattat gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa gtgatgagat
     8821 taagaggata attcgtgcgt tttgccatcc ggcgcacgat tattcagcac atcgtccagt
     8881 tttttgtggt ccagttcttt cacccactta gcaacgacaa tggtcgctac gccgttaccg
     8941 accaggttag tcagcgcacg agcttctgac ataaagcggt cgataccgag gatcagcgcc
     9001 agacccgcta ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac gataaagcca
     9061 ctacccgtta cccctgccgc ccctttagaa gaaagcagca acacgattaa cagcgtgatt
     9121 tggtggacga tatccatctg actgttagtg gcctgggcga taaacaccgc cgccattgtc
     9181 aggtatatcg atgtgccatc aaggttaaac gagtagcctg tcgggatgac cagccccacc
     9241 accgatttac ggcagccgag tttctccatc ttgtcgagca tacgcggcag cgccgactcg
     9301 gaagatgaag tccccagtac aatcagcagt tcttcacgga tgtagcggat aaatttgaag
     9361 atactgaaac cagtcgcttt agcgattgaa cccaatacca gcaccacaaa caggatacag
     9421 gtaatgtaga aacagataat cagctgcccc agttgcacca gtgtgccgac gccgtattta
     9481 ccgatggtaa acgccattgc cccgaacgca ccaataggtg ccagacgcat gatcatattg
     9541 atgatgccga agatgacctg cgagaaactt tcgatgacgt taaaaatcag ttggcctttg
     9601 ctgcccagac ggtggagcgc aaaaccaaac agtacggcaa acagcagcac ctgcagaatg
     9661 ttaccgctgg caaatgcgcc aatgacgctc gccgggatga catccataat gaaggcgaca
     9721 atgccctggt ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc aagcgttgcc
     9781 ggatcgacgt tcattccggc accaggctgc acgacgttaa cgatgataag accaataatc
     9841 agcgcgatgg tactgacaat ctcaaagtaa agcagtgcga ctgcgccggt acgaccgacc
     9901 gccttcatgc tttccatgcc cgcaatgccc gttacgacgg tacaaaagat gacaggagcg
     9961 atgatcatct taatgagctt aacgaagccg tcgccaagcg gtttcatttg ctcgcctatt
    10021 tcaggataga aatggccaag gagaatacca atggctatcg ctgtcaggac ctgaaagtaa
    10081 aggcttttaa acagagaggt tttcataggg tgtcctttag taaaaccaca ggtcttgtaa
    10141 ggttatgggg tacctgcggc cttaaaataa cacccagaca acatcacaga aatgtacctg
    10201 gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa ccagcacttt
    10261 ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc tcttcgaaga
    10321 tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg cccgcttttg
    10381 ccagccagtc gcgttgtgct tcagtctcca cgccttcggc aatcatttgt aagttcaggc
    10441 tctgcgccag catgataatt gcagcaatca tgctgctatc ttccggcaag ccttcaacaa
    10501 acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc agctgacgca
    10561 gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca ttgcgcagcg
    10621 gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct gtcacttcca
    10681 gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata tccgccacca
    10741 tatttgggtg catcagttgc agcgcagaga ggtttaccga caagggcagc ataatgccgc
    10801 gctcttgcca ggctgcaagc aatcgacagg actcttccag cacccagtga ccgacggtaa
    10861 ccatcagccc acagcactca atgcgatcga ttaagccatc cggcaggtcc caactgccat
    10921 ccggttgctg gatacgcagt aacacttccg cactgaccag ttttccgctg gtcatctcga
    10981 cctgtggctg taaccaaata gcaaactgat gattttccag tgcattaagg atatcgctct
    11041 cttccgtcaa ccgcttctgg gcggcttcca tctgctgcgg atcaaagaac tgaatctgat
    11101 tcttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa agctgttcgg
    11161 cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg agttgaatac
    11221 gttcaatcgg caggcgctcg ctcatgatag tgagcacttg ctgacctaag gtgattgcgt
    11281 gccacggttc ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta atctgcgcga
    11341 ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc agcagaattt
    11401 ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag gtgatgatca
    11461 tcagcgcggt ggtttgttta cgcgcgacaa cctgctccag catctccatc agcaaggctt
    11521 tgttcggcaa atccgacacc gggaagcgca tcgcattttc gttctgttct tcataatggc
    11581 gctgcagcaa ttgctggttg aggttgtaac tgcgcaccaa cataccgatt tcatcgtcct
    11641 gatgcagacg cggtaatgcc agttggtgac caacaagctc ctgggctggg atggcgttaa
    11701 gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac cagctgatgg
    11761 cgacggtcag gataagcgac aaaagtaagt aaatggtcac taacgttgag agggcgctca
    11821 tcacgaactt atacatacgg aaggaatccg cctgtagtac cagataggca attggctgcg
    11881 ggtttgccgg acgttcgagc gagtaaacgc ccagcgagat ttgaaccggt agctcaaaca
    11941 ggcgagtaac cattaccggc accgggcgct ctggaataaa acttttacgc agcgcctgga
    12001 actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta atgctggcaa
    12061 gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag ggttcgcgga
    12121 cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgt tggaccagat
    12181 ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct gccaccattg
    12241 ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc ggaaatccca
    12301 gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat ctgaaaactt
    12361 accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc tgccatccgg
    12421 cggcggaata acgtacgtag agcaggaaat ggctgggtgc gtaatctttc gcctgctgga
    12481 tatcaattgc cgtgccaaca aaccagttgg aagtaacacg tcgttcaagt aatgcccgcg
    12541 ccgtgtagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca gcttcttcct
    12601 gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg gtgcgtgaat
    12661 gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc tcccgccaca
    12721 tcaccggtat ggcaaacgac aggtattcct gcgggctgta gtaaccgccc tgaccgagtg
    12781 agtagccact caggtctttg tcgtaatgcc agatcatgtt gttcaggccg attgtgacgc
    12841 ggcgattgtt ctggttgatg accttatagt aatagcccgt catccagcgc acgcgccagt
    12901 tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag acgccgtttg
    12961 cttcaccttt atcgtagctc aggcttagcc ccacgccgtc ggcacgcaca ccgccccatt
    13021 ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa gaactggaga
    13081 tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg ctgtaactga
    13141 tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtgccgata tcccagctcc
    13201 agacgtcatt tcgccagccg accgccacgc tggcaccgga atccgactgg ctgcggttgc
    13261 cgctacagtc ctgtaatgta caggtgcccc agttatcatc ccatttacca tcggcattag
    13321 tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc cgcccgtcag
    13381 aatacggcgc gtccacctgc aacatagtgg tgtgcgcttt cagatcggag taaccaccgg
    13441 tgccgctcga tccccaataa tcatgctcaa gggtgacgtt aagatcctgc tggcgataga
    13501 ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg tcgttacggg
    13561 tcagtcgggt gaatgtgtcg ttatcctgcg gacgcgtcgt agtcacaccg gatgcgacca
    13621 tggcgtcttt gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct tcaaacttcg
    13681 caccgtcgcg cagcaccatc gcgctttcca tcgacggtgg ctgagatttt gcctgcggga
    13741 tcaacttatt aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc tgcgccagcg
    13801 ccacgcgccg ctgtgtgttc agcgaggcgt tatcggtagc gggcagtttc gccagctggc
    13861 tacgtgccgc cgctgtgtca ccggcagcaa tatccacttc cgtcagcccc agaatggcat
    13921 cggcgttagt tggctcccgc gtcaggacat tctgatatgc agcgcgggcg gcggtgtaat
    13981 cacgtcgctg ctgcgcccag tcagccagcg tgaggtcaat acgcgtggaa ggtggttgct
    14041 gacgcagcat cgcttccgct tcagcttctt tgccgctttc tcgcaggcga ttagcggttt
    14101 ccagcacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg ttccactgcg
    14161 cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc gtggtcatga ccagagaggt
    14221 acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc agattgcgca
    14281 ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgcgaa agtcggtaag
    14341 taatccatac gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct gccgcctgcg
    14401 cccatttgcc ctggttttcc agtgcctctg cctgctgtgc cagacggtcg ttttgcaggc
    14461 tgcgttcgat atcatcaatg ctacgccgct gactggcaga gagcgaggcg ataaacgctt
    14521 cagctttttc tggcgattgc tggcggtaaa tatttgccag cccgcgcacg gcgttagtgt
    14581 tgccgctgtc catacgcaag gtctgctgat aataacgttc ggcggcggga taatcttttc
    14641 gcgccatcgc cacatcgccc agccccagca ctgcataact gtcggtgtta tcgacattac
    14701 gcgcctgctg gaacaggcgt tctgcccggt caggattatt ggctttcagc gcagcatcgc
    14761 cctgctggat cgccagccag tagcggttta ctttcagcag actgttccat ttgtcgttgt
    14821 tgctgctgtg cgggtccagt gcgagggctt tttccagatt cgccactgca ttggcgcgat
    14881 cgcctttctg agaatacgcc tggcccagcg cccccagagc ttcactgtct ttcgggttcg
    14941 cccgcaccgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag tccaccgccg
    15001 ctaaaccttg cgcacgagcg cggaaagcag gatcggccag ctgtttttgc tgttctgcca
    15061 gttgcgattg cgcagccgcc acgctatcgc catcactaaa gatcgagaga tattttttca
    15121 gcgccgacac actggcatca ctgacgggca tgtctttaat ctgcccgtac cagattttag
    15181 aggccccttc gcgcccggcg ttcgattttg ccatctgttc caggacggca aaaccttcgt
    15241 cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc gtattgcccg
    15301 gtgcatccgc attgatgcgt tttaactgat taatcgcttc gccacggcga gccggaattt
    15361 tcgccaccgt actccagtac tcgacagcaa tgtcaccttc cggcggcgca ccgttgaaca
    15421 gtttgttgta actcgccaca gcttcttctg catgaccggt cgtcgcctgc aatcgtgcct
    15481 gttgcagtgc ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc gacgatttat
    15541 acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc gcgccatcaa
    15601 tatcgccctg acgtaacaaa gaacggaaac gggcggcaac gacgtccggg ttattcggat
    15661 caataagttc cagccgatat aacgactgtt gcaccagatc ttcacgatgg gtcgcttcgc
    15721 ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg accatcggca
    15781 tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc attcctggcc
    15841 ccagtcaggt aataactcac cttttgtcga gaagcggaaa cggtgttgat cccagccttg
    15901 tccaaacagg gtcagcacat agttgtaata ggcatcgctg ccgggaaagt tatcggccac
    15961 gcgctggcgc tgaacggcct gcgcatcgcg gttttgtaaa aagggcagca tggcggcaga
    16021 aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt tttccggcgg
    16081 ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca tccgcgcttt
    16141 ttgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag cgtcgtagct
    16201 gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat agcgcaccca
    16261 gtctggcgaa aaacctttcg gggcggtttc cagcaataaa cgttgattgg tttcgcgcag
    16321 cgtggtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg gcaggtagct
    16381 ggggttaaaa cgccagctgt tatcctcagc aaaacccact ttgcccggta acaacatgga
    16441 acccagccca ggcaccgtca ccacttcctc ccgcgcgata cgttttagca acgcgctgcc
    16501 gatgtcggta taacgctgct ctttccacaa acgccccgcc tccagcaacg accaggccat
    16561 ccagacatca ccatcggagg ccgaattgct gtccagcact tcccacttac tgttctcttt
    16621 cttgccccac agccaggcgg gcaaacgttc ttttaaagaa ccctgagcga gattgttctg
    16681 cgtccagtcg agaatattat cgaaagctac acggtcgtta gccgccaggg caaagaacat
    16741 accgtaactt tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt cgatgacgcg
    16801 cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggccagg tacaggctg
//
LOCUS       DAFATA010000097        16776 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFATA010000097 DAFATA010000000
VERSION     DAFATA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.098"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..451
                     /gene="gstA"
                     /locus_tag="LGJ20_003737"
     CDS             <1..451
                     /gene="gstA"
                     /locus_tag="LGJ20_003737"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBK2387751.1"
                     /translation="PALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIE
                     WLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIA
                     DAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(495..1358)
                     /gene="pdxY"
                     /locus_tag="LGJ20_003738"
     CDS             complement(495..1358)
                     /gene="pdxY"
                     /locus_tag="LGJ20_003738"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBK2387752.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1417..2691)
                     /gene="tyrS"
                     /locus_tag="LGJ20_003739"
     CDS             complement(1417..2691)
                     /gene="tyrS"
                     /locus_tag="LGJ20_003739"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK2387753.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2820..3476)
                     /gene="pdxH"
                     /locus_tag="LGJ20_003740"
     CDS             complement(2820..3476)
                     /gene="pdxH"
                     /locus_tag="LGJ20_003740"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBK2387754.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3535..3864)
                     /gene="mliC"
                     /locus_tag="LGJ20_003741"
     CDS             complement(3535..3864)
                     /gene="mliC"
                     /locus_tag="LGJ20_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBK2387755.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(3962..5071)
                     /gene="anmK"
                     /locus_tag="LGJ20_003742"
     CDS             complement(3962..5071)
                     /gene="anmK"
                     /locus_tag="LGJ20_003742"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBK2387756.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5345..5812
                     /gene="slyB"
                     /locus_tag="LGJ20_003743"
     CDS             5345..5812
                     /gene="slyB"
                     /locus_tag="LGJ20_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBK2387757.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5859..6293)
                     /gene="slyA"
                     /locus_tag="LGJ20_003744"
     CDS             complement(5859..6293)
                     /gene="slyA"
                     /locus_tag="LGJ20_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBK2387758.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6494..6730
                     /locus_tag="LGJ20_003745"
     CDS             6494..6730
                     /locus_tag="LGJ20_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBK2387759.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6733..7590
                     /locus_tag="LGJ20_003746"
     CDS             6733..7590
                     /locus_tag="LGJ20_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2387760.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7590..9602
                     /locus_tag="LGJ20_003747"
     CDS             7590..9602
                     /locus_tag="LGJ20_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK2387761.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9603..10124)
                     /gene="sodC"
                     /locus_tag="LGJ20_003748"
     CDS             complement(9603..10124)
                     /gene="sodC"
                     /locus_tag="LGJ20_003748"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBK2387762.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10205..11101)
                     /locus_tag="LGJ20_003749"
     CDS             complement(10205..11101)
                     /locus_tag="LGJ20_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBK2387763.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11150..11389)
                     /locus_tag="LGJ20_003750"
     CDS             complement(11150..11389)
                     /locus_tag="LGJ20_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBK2387764.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11492..12091
                     /gene="nemR"
                     /locus_tag="LGJ20_003751"
     CDS             11492..12091
                     /gene="nemR"
                     /locus_tag="LGJ20_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBK2387765.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            12128..13225
                     /gene="nemA"
                     /locus_tag="LGJ20_003752"
     CDS             12128..13225
                     /gene="nemA"
                     /locus_tag="LGJ20_003752"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBK2387766.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13306..13713
                     /gene="gloA"
                     /locus_tag="LGJ20_003753"
     CDS             13306..13713
                     /gene="gloA"
                     /locus_tag="LGJ20_003753"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK2387767.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13816..14463
                     /gene="rnt"
                     /locus_tag="LGJ20_003754"
     CDS             13816..14463
                     /gene="rnt"
                     /locus_tag="LGJ20_003754"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBK2387768.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14556..>16776
                     /locus_tag="LGJ20_003755"
     CDS             14556..>16776
                     /locus_tag="LGJ20_003755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK2387769.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAITGRIHALWGADASVVASDDGIVA
                     "
BASE COUNT         3912 a         4430 c         4295 g         4139 t
ORIGIN      
        1 gcctgcattg ctgctggatg acggtacttt gctgacggaa ggcgtagcga ttatgcagta
       61 tcttgccgac agcgtccccg accgccagtt gctggcaccg gtaaacagta tttcccgcta
      121 taaaaccatc gaatggctga attacatcgc caccgagctg cataaaggtt tcacacctct
      181 gtttcgccct gatacaccgg aagagtacaa accgacagtt cgcgcgcagc tggagaagaa
      241 gctgcaatat gtgaacgagg cactgaagga tgagcactgg atctgcgggc aaagatttac
      301 aattgctgat gcctatctgt ttacggttct gcgctgggca tacgcggtga aactgaatct
      361 ggaagggtta gagcacattg cagcatttat gcaacgtatg gctgaacgtc cggaagtaca
      421 agacgcgctg tcagcggaag gcttaaagta atatccatcg gcccacaggt cgttgtgggc
      481 cgaaatgaga tatttcagag ctttgttgcg ctgaagtaat gttctggttt ggcaatacga
      541 tcctgagcag ccaccacttg cagctcatat tcctgcattg ctttggtggt caccatgatt
      601 tcgtagactg cagcggtcac atgttccagc gcctcctgca gcgttgcccc ctgaagcagt
      661 ttcaccagca gtaaaccgct cgtcacatca ccaacaccta ccggctggcg cataccaaaa
      721 tccaccagcg gacggctgat atgccaggct tcatcggcgg tgaccagcag catttcaaaa
      781 cggtcacggc tgtagccagc tcgcgccagg tgtttaacca acacaatttg tggcccttgc
      841 gcaatgagtt cgcgcgctgc cagaaccgct tcttcgacgt tatttaccgc atgctcacag
      901 agtatttcca gctcaaccag attcggcgca atgatatcgc tggcaggcaa accgtgccgc
      961 acatgaaact ctgcgacacc cggtgcaacg atacagcctt tttccggatg acccattacc
     1021 ggatcgcaaa aatatttcgc ctgcggattc gcggctttca cctgacggac gataccgagg
     1081 atatgttcac cctgctccgc cgatcccaga tagccactta acacggcatc acaggtgtgt
     1141 aatttatcaa tggcggcaat gccttgcaca atttcggtta aatggctggg cggcatcacg
     1201 cagccagtcc atttgccgta ttgggtgtga ttagaaaatt gaacggtgtt cagtggccag
     1261 acgttcgcgc ccagacggcg catcggaaac tctgccgcac tgttacccgc atgaccataa
     1321 acaacgtgag actggatagc gagaatattt ttcatcatgt tccctgtata aaaaccaggg
     1381 ggagtgattt ctcactcccc ctttccactt aatgcattat ttccagcaaa tcagacagta
     1441 attcttttta ccgcgacgca gtaaggtaaa acgaccaaac agacgatctt cttctttaaa
     1501 gaagtattca ggatcggact gtttttcacc gttaatggtg atggcattgg aggcgatagt
     1561 tttacgtgcc tgaccacggg aaggttgcag ttcagaatcg accagtgcct gcatcaggtc
     1621 tgcgcccttt tccatctcaa ccatcggtac gccgtcctgc gccagctgtt cgaagtccgc
     1681 ttcactcagc gcactcaaag aaccgctgaa caggcattcg gtaatacgtt ttgccgcctg
     1741 taaaccttct tcaccgtgaa ccagacgagt cacctgctcc gccagtacat actgggcgcg
     1801 cggtgcttta ccgctgtttt tatcttcttc ttccagggcg ttgatctctt caatgctcat
     1861 aaaggtgaag aacttcagga agcggtaaac gtcggcatcc gcagtgttga tccagaactg
     1921 gtagaatttg tacgggctgg ttttcttcgg atccaaccag actgcgccgc cttcagtttt
     1981 accaaatttg gtgccatctg ctttagtgat cagcggaacg gtcaggccaa acacctgatt
     2041 ctgatgcaga cgacgggtca ggtcgatacc agaagtgatg ttaccccact ggtcagaacc
     2101 accaatttgc agcaccacac cgtactgttt gttcagacag gcgaagtcat aaccctgcaa
     2161 caggttgtag gaaaactcag tgaacgaaat cccctgatct tcacggttga gacgctgctt
     2221 aaccgcttct ttgttgatca tctggttaac ggagaagtgt ttgccaatat cgcgcaggaa
     2281 ggtcagcaca ttcatattgc cgaaccagtc atagttgttc gccgcgatag cagagttttc
     2341 tccacagtcg aaatcgagga acggggcaac ctgcttacgg attttgtcca cccactcctg
     2401 aacagtttct tcggtgttca gcttacgctc ggcagctttg aagctcgggt cgccaatcag
     2461 acccgtcgcg ccgcctacca gcgcaaccgg cttgtggccc gcctgctgga agcgtttcag
     2521 gcataacaat ggaacaagat gccccaaatg caagctgtca gcggtaggat cgaagccgca
     2581 atagagcgcg atcgggcctt gcgccagtcg ctctgctaac gcttcctcgt ccgtcacctg
     2641 ggctaccagc ccccgctctt gcaattgttt aatcaagtta ctgcttgcca tcaaaatctc
     2701 catgtatata acgactgcac ctttgccggt acacgacttt tcgccagatg cgaaagagac
     2761 atagaataaa gtgccagaat caggagtacc agcgattaaa gcaagatttt tgcatctttt
     2821 cagggtgcaa gacgatcaat cttccacgca tcattttctc gctggtacaa aaagcggtca
     2881 tgcaggcgat gctcaccgcc ctgccagaac tcaatctgtt caaggctgac gcgaaaaccg
     2941 ccccaaaagc tcggcaatgg cacttcgccc tgttgaaact tctgcttcag ctccaggaat
     3001 ttactttcaa ggataccgcg ggcagaaatg cgactggact gcttcgaaac ccatgcacca
     3061 atctggctat cacgcgggcg gctatgaaaa tatttcatca cttcgagagt cgaaagtcgt
     3121 tctgctttac cgatcaccat cacctggcgt tcaagggtat gccacgggaa cagcaggcta
     3181 acgcgcggat tattttcaat ttgatgtgct ttacggctac cgaggttggt gtaaaacacc
     3241 atgccttttt cgtcgtaatg tttgagtaaa acgatgcgtt gataaggctg agcatgttca
     3301 tccacggtag cgaccaccat cgcggtaggg tctgccagtt tggcttcaca cgcctgagag
     3361 agccaacgtt caaaaagggt taatggatcg gcgggaagat cgcgacggcg taacccgcct
     3421 ttggtgtatt cacggcgcag atgcgcgatt tgctgcaatt cgtcgttatc agacatggtt
     3481 ttctttacgg attgtcagtg ggtgacgcta ttgtgcgccg cccctggaaa aatctcaacg
     3541 ctgtggattt tgtaactgac agttattcaa tacgatgcgg tcgcgtttat agacagtcgc
     3601 ttcatcgcct ttcgaccaga aaacatagat tccgtcagtg taacgtgcac cagacgctga
     3661 aatgccctgt ttgagatgca gtagttgatt atcgtaaaca aaactgactt cctggcgcgg
     3721 attattcagt ttgaccgtca acggtttttc atcacactgg tattccagcg tagcggtttg
     3781 catgtgctca accagttgat tgaacgcgct acagccggtg agaagcaccg gcaaacagat
     3841 cagtaacagt tttttcatag acatttcccg aagactttcc tggtctggag ggcaatacgc
     3901 cctccctaac gttccaagtg taacggcaga cgcggtaaga aaaattcagt taactctgat
     3961 atcacgggtt agcagggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg
     4021 aaggcagatt tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg
     4081 cttccatgtc atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctggca
     4141 gtaatgccgc cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca
     4201 atcgttcgca gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg
     4261 ccagtgtggc ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca
     4321 gccagccgta gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatatg
     4381 ggtcgctgag catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg
     4441 catctttatc gtaaggttta ccggcctgac gccagatcca ggcatccatc agcatgttac
     4501 caggaccggt atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga
     4561 tgccgccaat attgagcacc attcgtcgct cggttgagtg agccagcaag gcatgatgga
     4621 acgcaggtac cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac
     4681 cgacaaccgt aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt
     4741 gtggtgccac gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta
     4801 tatctctcgc ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc
     4861 gcccgagttg agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa
     4921 tatccagtac agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg
     4981 cgacccggtg ttcatctatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg
     5041 acataacgcc aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc
     5101 gcaccactca aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg
     5161 gtagtaaaac attatcttca aatcaataat catcatgaat gttttgttta taattggttg
     5221 atcctacttt cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata
     5281 tgccatatac tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt
     5341 ttcaatgatt aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt
     5401 taataacgac accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa
     5461 tgtcagctat ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc
     5521 caacgttatc ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg
     5581 tggcggaacc gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg
     5641 tcagggcgta cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga
     5701 cgatggtaat accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca
     5761 acgtgtcgta ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt
     5821 gcgtgtggtc aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca
     5881 atgatattat gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag
     5941 atgccatgta atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt
     6001 ggctctgcct tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga
     6061 cgcgaaatta accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg
     6121 atgccaatcg cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg
     6181 tgtaacgtaa cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga
     6241 gcacgccata tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc
     6301 tccttataat tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta
     6361 ttgctaaaac aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc
     6421 gcgtaaattt ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa
     6481 aggaatctac tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt
     6541 gttcggtgcg tccgtctatt ttcctccgtt tttcaaagcc ttcgcgtttg gattcgtcat
     6601 ctggcttgtc gtacaccgcc tgcttcgtgg ctggatctac gccggtgaca tctggcatcc
     6661 cttgttaatg gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat
     6721 tgcgtggtaa ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata
     6781 gttgcggttc ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc
     6841 gacgggaaaa tacgcgcaga aaaggtttct atcaccccac aggtgtcggg acgtattgtt
     6901 gagctgaata taaaagacaa ccaactggtt aacgcggggg atcttctgct cacgatcgat
     6961 aaaacgccct ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct
     7021 gacctcgcaa aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt
     7081 tctgccgaag aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat
     7141 gccgcacagg ccacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc
     7201 ccggtgagtg gttgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg
     7261 aaaccgctgt ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa
     7321 accaagttgc gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac
     7381 aaaacgttac ggggtcacgt ttccagtatc ggtcgggcga tttatgatca aagcgttgaa
     7441 agtgactcaa gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa
     7501 cgcgttcccg tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc
     7561 accacctgta gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt
     7621 tgccctggtt caaggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca
     7681 tgtgtctggc gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga
     7741 cctcggctgc agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac
     7801 gcatcgctgg cagtttactg ggagccattg cggcactgct tcttgccggg catacgctca
     7861 atgagccgtg gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctgggcct
     7921 gtgcgcactt cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca
     7981 tcatcgcctt tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg
     8041 cgcgcgtttg cgaggtgata gtcggtattt tgtgcggcgg aatgatgatg atgatcctgc
     8101 ctagcagttc cgatgctaca gcccttttaa ccgcattgaa aaacatgcac gcccgattac
     8161 tggaacatgc cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg
     8221 agggggtgat tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc
     8281 actatcgttt tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta
     8341 tgaccagtgt catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg
     8401 ccacgcgaga aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt
     8461 acaccgtcgc acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg
     8521 tcgccttctg gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat
     8581 tacatcgtct gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg
     8641 ctcgtcatac cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga
     8701 tgatgattgg cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc
     8761 tggcagcaat tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac
     8821 tgctgatgcg cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga
     8881 tggtccagat tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa
     8941 tgcagcttct taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt
     9001 ttatgggttc gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta
     9061 acgataatct ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc
     9121 gtccaggatc ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg
     9181 tcgatcagct aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc
     9241 atcacgtcag tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct
     9301 ggggtgtagt gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc
     9361 gttccgatcc gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga
     9421 gtgagcgtgg cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt
     9481 gcgacagcct tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc
     9541 ggctgtactg ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt
     9601 aattacttaa ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga
     9661 tcggacatat tatcgccgcc aacgtggacc atcagcgctt tgtctttgat ttcatccagt
     9721 gatttcagac gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt
     9781 gcaggcagat cgcctaaatg cccggcacct tctggccctt catgtttacc ggtattttgt
     9841 ggatcaagat gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc
     9901 tggcagcttc ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga
     9961 tcgggcgaaa actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc
    10021 cctacccctt gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg
    10081 gttgcaacaa ccagcgccag aatagccaga ctaaaacgtt tcataggacc tccgttcatt
    10141 gtgcattcct gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa
    10201 gtgcttacgg tacgtcgtac cccagtgccg ctttacggat acgaaaccat tgttgacggg
    10261 tcattttcag tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgaaccga
    10321 taattggcag cggctgcgat ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg
    10381 agcccgcgtt taactcctct gccaccacag ccagttcatc acgcagctgc tggaaataat
    10441 catcattaaa cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt
    10501 gttgtagttg gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca
    10561 cctgattagt ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg
    10621 taaagttcga tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt
    10681 ccgccacttc atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat
    10741 gatcggtcgc gagattaatt agcgactgtt cggcgctctt aatgatgtga tcgcggtcag
    10801 tgatgtaatg accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga
    10861 cgatttccat ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt
    10921 cgcactgata gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat
    10981 gctcttcaat aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt
    11041 agcccatcac aaaacgggaa aactccgggc cttgcggcgc aatagtaata cgctgaacca
    11101 taatcgcttc ctcttatcag atatgagagg agtatacgca agattaggtt caaaagagtg
    11161 atggttgctc cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc
    11221 gctgccgaca aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa
    11281 aacgctcatc ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa
    11341 ttccccggca cgggctctgg acgggaaaga actctaattg ctccgccact tcgccctcct
    11401 cagataagat tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca
    11461 ctagaccgac tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg
    11521 cgaacatctc ctggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg
    11581 gctaagcgaa ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg
    11641 ctctaaagaa gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg
    11701 actgactgag ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta
    11761 ccagcaaaca ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa
    11821 actctctgcc gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc
    11881 tcgcggcgtg atcgccctgc tctctcaggc gctggaaaat ggccgtgaga accattgttt
    11941 aaccttttgt ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggctgggtgc
    12001 gaatctgcag gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt
    12061 aaaaaacatt attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg
    12121 agcctttatg tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc
    12181 aaaccgtatt tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc
    12241 taccccgttg atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga
    12301 agccacgcaa atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc
    12361 ggagcaaatt gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat
    12421 ggccgtgcag ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca
    12481 ggcaccggta gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa
    12541 tggtcaggcg atccgtgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc
    12601 aggtatcgtc aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg gttttgatct
    12661 ggtagagctc cactctgctc acggttattt gctgcatcag ttcctttctc cttcttcaaa
    12721 ccatcgtacc gatcagtacg gcggcagcgt ggaaaatcgc gcacgtctgg tactggaagt
    12781 ggtcgatgcc gggattgaag aatggggtgc cgatcgcatt ggcattcgca tttcgccaat
    12841 cggtactttc cagaacacgg ataacggccc gaatgaagaa gccgatgcac tgtatctgat
    12901 tgaacaactg ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg
    12961 gggtgaaccg tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat
    13021 tatcggcgca ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat
    13081 tgatgcggtg gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca
    13141 gcgcaaagct gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg
    13201 ctataccgat tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata
    13261 ctaaaacaac attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat
    13321 accatgctgc gcgttggcga tctgcaacgc tccatcgatt tttataccaa agtgctgggc
    13381 atgaaactgc tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc
    13441 tacggcccgg aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa
    13501 tacgaactcg gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg
    13561 tgcgaaaaaa tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc
    13621 ggtactacgg ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa
    13681 gagaaagacg ccggtcgcgg tctgggcaac taatctcctg ccgggcgtga actcatcgcg
    13741 cccgcatctt tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg
    13801 tattaagaga atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt
    13861 ggtttttatc ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg
    13921 ctgcttgaga ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac
    13981 accacattac atttccacgt cgaaccattt gtcggcgcaa atttgcagcc agaggccctc
    14041 gccttcaacg gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg
    14101 ctgcacgaaa tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc
    14161 attatggtgg cgcacaatgc caattttgat cacagcttta tgatggccgc cgcagaacgc
    14221 gcctcactga aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc
    14281 gggctggcac tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc
    14341 gacagcaccc aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt
    14401 gaaatcgtca accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg
    14461 taatcgagtc gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca
    14521 cgcatcctgc atgatattca cggggaatag cgttaatggc agataatcca gacccttcat
    14581 cgctcctgcc ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac
    14641 agccgaccgc tgtccagtcg caaacctggc atgtggcggc gcgaagcgaa catgcgctgg
    14701 tgattgcacc gaccggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc
    14761 tcttccgcga aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc
    14821 tctatatttc accgataaaa gctctgggca ccgacgttca gcgcaacttg cagctcccgt
    14881 tgaagggtat tgccgatgaa cggcggcggc gcggcgaaac ggaagtcaat cttcgcgtag
    14941 ggatccgtac tggcgatacg cctgcacagg aacgcagcaa actcacccgt aatccgccgg
    15001 atattctgat caccacaccc gaatcactct atctgatgct gacctcccgc gcgcgcgaaa
    15061 cgctacgcgg cgtcgaaacg gtaattattg atgaagtcca cgcggtagcg ggcagtaaac
    15121 gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac acctcagcac
    15181 agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca tttcttggtg
    15241 gcgatcgccc ggttacggta gtcaacccgc ccgcaatgcg ccatccgcag atacgaattg
    15301 tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcagcacc ggcgaagaca
    15361 gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc cttgatgaag
    15421 tgttgcgcca tcgctcgacc attgtcttta ctaattcacg tgggctggcg gaaaaactga
    15481 cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct atcgccgttg
    15541 atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa agtagcgacg
    15601 tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca atcaccgagc
    15661 aggcgctgaa atcgggtgag ttacgctgcg tggtcgcaac ctccagtctc gaactgggga
    15721 ttgatatggg cgcagtggat ctggtgattc aggtggcaac gccgctttct gttgccagtg
    15781 ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa gggctgtttt
    15841 tccctcgtac ccggcgtgac ttagtcgatt ccgcagtcat tgtagagtgt atgttcgcag
    15901 gcaggctgga aaacctgaca ccgccgcata atccactcga cgtccttgcg cagcaaaccg
    15961 ttgccgccgc ggcgatggag gcattacagg tagacgaatg gtactcccgc gtacgccgtg
    16021 ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat atgctttccg
    16081 ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg aacagggaga
    16141 ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc ggcggcacca
    16201 ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag gccggttcgc
    16261 ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac gacattatca
    16321 ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg attgtgactc
    16381 ctgctccggg tcgttctgcc cggctcccct tctggcgtgg tgaaggtaac ggacgtccgg
    16441 ctgaattagg cgagatgatc ggcgattttc ttcatttgct ggcggatggc gcgttctttt
    16501 ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt caggggttga
    16561 ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg gtcctcgaac
    16621 ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat ggaagacggg
    16681 tgcatgaacc ctgggcgctg gcgattaccg ggcgaataca tgccctatgg ggcgctgacg
    16741 cgtctgtggt cgccagtgat gacggcattg ttgcgc
//
LOCUS       DAFATA010000098        16718 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFATA010000098 DAFATA010000000
VERSION     DAFATA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.099"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1410
                     /locus_tag="LGJ20_003756"
     CDS             <1..1410
                     /locus_tag="LGJ20_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HBK2387770.1"
                     /translation="QLSGIGTTLTGINFFVTILKMRAPGMTMFKMPVFTWASLCANVL
                     IIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYINLIWAWGHPEVYILILPVFG
                     VFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHHFFTMGAGANVNAFFGITTMI
                     IAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFSVGGMTGVLLAVPGADFVLHN
                     SLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNETWGKRAFWFWIIGFFVAFMP
                     LYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALGILCLVIQMYVSIRDRDQNRD
                     LTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWEMKEKGEAYKKPDHYEEIHMP
                     KNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIITWIVKSFDEDVDYYVPVAEIE
                     KLENQHFDEITKAGLKNGN"
     gene            1400..2014
                     /locus_tag="LGJ20_003757"
     CDS             1400..2014
                     /locus_tag="LGJ20_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBK2387771.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            2014..2343
                     /locus_tag="LGJ20_003758"
     CDS             2014..2343
                     /locus_tag="LGJ20_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBK2387772.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            2355..3245
                     /gene="cyoE"
                     /locus_tag="LGJ20_003759"
     CDS             2355..3245
                     /gene="cyoE"
                     /locus_tag="LGJ20_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBK2387773.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            3430..3741
                     /locus_tag="LGJ20_003760"
     CDS             3430..3741
                     /locus_tag="LGJ20_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBK2387774.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            3725..4267
                     /locus_tag="LGJ20_003761"
     CDS             3725..4267
                     /locus_tag="LGJ20_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2387775.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(4323..5258)
                     /locus_tag="LGJ20_003762"
     CDS             complement(4323..5258)
                     /locus_tag="LGJ20_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBK2387776.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            5666..7030
                     /locus_tag="LGJ20_003763"
     CDS             5666..7030
                     /locus_tag="LGJ20_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387777.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(7158..7649)
                     /gene="yajQ"
                     /locus_tag="LGJ20_003764"
     CDS             complement(7158..7649)
                     /gene="yajQ"
                     /locus_tag="LGJ20_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBK2387778.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7817..8728
                     /gene="panE"
                     /locus_tag="LGJ20_003765"
     CDS             7817..8728
                     /gene="panE"
                     /locus_tag="LGJ20_003765"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK2387779.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGAIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8691..9281
                     /gene="yajL"
                     /locus_tag="LGJ20_003766"
     CDS             8691..9281
                     /gene="yajL"
                     /locus_tag="LGJ20_003766"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBK2387780.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(9335..10783)
                     /gene="thiI"
                     /locus_tag="LGJ20_003767"
     CDS             complement(9335..10783)
                     /gene="thiI"
                     /locus_tag="LGJ20_003767"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK2387781.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10989..11231
                     /gene="xseB"
                     /locus_tag="LGJ20_003768"
     CDS             10989..11231
                     /gene="xseB"
                     /locus_tag="LGJ20_003768"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK2387782.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            11231..12130
                     /gene="ispA"
                     /locus_tag="LGJ20_003769"
     CDS             11231..12130
                     /gene="ispA"
                     /locus_tag="LGJ20_003769"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBK2387783.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            12155..14017
                     /gene="dxs"
                     /locus_tag="LGJ20_003770"
     CDS             12155..14017
                     /gene="dxs"
                     /locus_tag="LGJ20_003770"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK2387784.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            14197..15171
                     /gene="yajO"
                     /locus_tag="LGJ20_003771"
     CDS             14197..15171
                     /gene="yajO"
                     /locus_tag="LGJ20_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBK2387785.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(15225..15743)
                     /gene="pgpA"
                     /locus_tag="LGJ20_003772"
     CDS             complement(15225..15743)
                     /gene="pgpA"
                     /locus_tag="LGJ20_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK2387786.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15721..16698)
                     /gene="thiL"
                     /locus_tag="LGJ20_003773"
     CDS             complement(15721..16698)
                     /gene="thiL"
                     /locus_tag="LGJ20_003773"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBK2387787.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
BASE COUNT         4016 a         4298 c         4312 g         4092 t
ORIGIN      
        1 cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac cattctgaag
       61 atgcgcgcac cgggcatgac catgttcaag atgccagtat ttacctgggc atcactgtgc
      121 gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc gttgttgacc
      181 ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa catgatgatg
      241 tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat cctgcctgtt
      301 ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc
      361 tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtttg gctgcaccac
      421 ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac cacaatgatt
      481 atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta tcagggccgc
      541 atcgtgttcc attctgcgat gctgtggacc atcggtttta tcgtcacttt ctcggtgggc
      601 gggatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca taacagcctg
      661 ttcctgattg cgcacttcca taacgtgatc atcggcggcg tggtcttcgg ctgcttcgca
      721 gggatgacct actggtggcc taaagccttc ggtttcaaac tgaatgaaac ctggggtaaa
      781 cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct gtatgcgctg
      841 ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt ccacaccatg
      901 ctgatgattg cagctagcgg tgcagtactg attgcgctgg gtattctctg cctcgttatt
      961 cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg cgacccgtgg
     1021 ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa ctttgccgtt
     1081 gtgccgcacg ttcacgagcg tgatgcattc tgggaaatga aagagaaagg cgaagcgtat
     1141 aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc aggtatcgtc
     1201 attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg gtggctggcg
     1261 attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga cgaggacgtg
     1321 gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt cgatgagatt
     1381 actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg cccacgcgca
     1441 cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga tctacctgat
     1501 gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg tgaacggcac
     1561 cgcaggcggt ccgacaggta aggacatttt cgaactgccg ttcgttctgg ttgaaacttt
     1621 cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt acaaaaacaa
     1681 caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg gatttatcgg
     1741 gatggaaatc tacgaattcc atcacctgat tgttaacggc atgggtccgg atcgcagcgg
     1801 cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca cttccggtct
     1861 tatctggatg gcggtgctga tggtacaaat cgctcgtcgc ggcctaacca gcactaaccg
     1921 tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt ggatctgtgt
     1981 gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga tcacagcggc
     2041 gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat cattctgacg
     2101 gtgattccgt tctggatggt gatgacaggg gctgcctctc cggccgtaat tctgggaaca
     2161 atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct gcacatgaat
     2221 accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct aatcatcgct
     2281 atcctggttg taggctccat ctggattatg tggaacctca actacaacat gatgatgcac
     2341 taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca ggcatcatct
     2401 ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc agcattgatt
     2461 atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt
     2521 ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag aatcgggtgc
     2581 tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg ctgggtattg
     2641 ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg ggggtgatgg
     2701 gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa acgccactca gtctacggca
     2761 cattgattgg ttcgctctcc ggcgctgcgc cgccggtgat tggctactgt gcagtaaccg
     2821 gcgagttcga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg cagatgcctc
     2881 actcctatgc catcgccatt ttccgcttta aggattacca ggcggcaaac attccggtat
     2941 tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat atcatcgcct
     3001 ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tggatataaa tatctggtgg
     3061 tcgccgcggc ggttagcgtc tggtggttag gtatggctct gcgcggttat aaagttgctg
     3121 atgacagaat ctgggcgcgc aagctgttcg gcttctctat catcgccatc actgccctct
     3181 cggtgatgat gtccgttgat tttatggtac cggactcgca tacgctgctg gctgctgtgt
     3241 ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct tttttattgt ctgagatcag
     3301 agatacggct aataaccgta tgtcttgaca gagtgagcag atgaacataa attaatcata
     3361 cggttgatag ccgtattatg tatgagatcg actccctacg atttcattca gttaatcaaa
     3421 gaggtcataa tgcctggaaa aacagccact ctacccgatg tcgataaaac gctgaaaaat
     3481 gcgcgggtcg aactcaaaac cagtcccgac gccaaaaaca aacttcgtga agctgcgcaa
     3541 gccgttgggg tagatttaag cgcttttatt ttgagcgctg ctatggaacg cgccgaaagt
     3601 gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa gctgggaact gatgaaccaa
     3661 ctcatcgctg aacctgctca accgacgctc gccctcaagg cgttaatgaa aaggaaaaac
     3721 agcgatggtc gacaagcatg aagagattac tctgcccata gtcctctcct gtaattatca
     3781 gtctgatatt acttatcctg ggcaaaaaca gtttgattgc ggtaaccctg ttatcgataa
     3841 atttgtacgc gcatcgctaa agaaaagtgt gcgtaatagc gactgtgcgg ctaaagcact
     3901 tattgacaga caaagtggtg aactgatcgg catctgtact tttacggcat attcgctgga
     3961 aaaacaacgc gtttctggcg tccttcaggg ttcacaacct tcagaaattg gtgttgtcag
     4021 attagtcatg ttgggggtag cacggaagta tcaaaagcgg ggctttggtc aggacctact
     4081 atgtgatttt tttgaacatg taaaaataat tcaccaggca ttaccaatta aaggggttta
     4141 tcttgatgct gaccctgccg ccattaattt ttatgctcgt ctcggctttg ttcagctttc
     4201 agcgacacca aatgcttttg gtgctgtacc tatgtttttg gcgattcagc atattctcgc
     4261 ggcttagatt attatcgcca cccgccaggg caagttttgc ctcagccggg ggctgagcag
     4321 cgttaccact tttgttcaat tgcccccgct ttcagcaact ccacatcctt gccgttaatg
     4381 gttgccgttg cggcaacgat tgtggtcctt acagttgcac aagattttcg ctcaaattcc
     4441 ggtttataaa gaataagaga cgaattactg gtaaatgaac caaataggta atcagtaaag
     4501 ctaatattca gccggtttaa accgaaaggc gcgaagccaa ccgtttggtt aatctcctta
     4561 ccggatgggc gacgaatgct gaatgtcacg cgaagattgg ccacttcgcc taatttattg
     4621 gtggtggcga agaggctatc catgcaggaa aaacctttat ctaaaaaatt atctcgcatc
     4681 ccagaacggg taaccgtaat cacgggtaac gtatcgtcaa ccgtttttct gtcaatcatg
     4741 tcttttcgac aagtagccag aaagtcttct gggccaccat aaatgtggac acctttatca
     4801 agatattcct tcgctttctt acaatctacc tcaacatact gaccattggc gtacataatg
     4861 cccaacctgg accatgctaa attcacaccg tgctgcgcgg cctgattaaa tagtgcatac
     4921 gctttggcat aatccacccg cacctgctca ccgtcgtagt agataccccc cagcgctaac
     4981 ggagcttctc tgtcaccttg tttagccgct aactcgaacc atttaatcgc ttcctgaaca
     5041 tcaggtttgg tatcataacg aggctgataa tattcccagc ctaacaggct ctgcgcgtcg
     5101 atatctcccg attcagccat tttatgcagt gtatttataa catcgggagc aggtttacca
     5161 ccgtatatgc tgtcatacaa ttcatgcgct ttctcgctgg ccaccacgct tggtgaaact
     5221 atcagcatta cagaaaataa tattctttta gttaacatgt atacctttat gtcctcactg
     5281 tcatatattt ccataataat taaccaccaa atatccattt aatgaataaa tacagtgcca
     5341 taaaggtcca agtaataagc acacatttag cctgaaacat tatcatccat gtcaccaaat
     5401 cgtttattta gtgagataaa actagcatat tcgtaagata aaaaatgcac atggcaatca
     5461 agaaatgata ttattacttt tttgatgtaa aactatttag ttttaaaatt cattttttca
     5521 acatatagag cattcatttt atgaatgcaa aaaaacatac ctgacacaca aattactctc
     5581 ctctgtaata ttttctcatt aacactctgt ttacccaaca tgcgctccgc actacactat
     5641 gtgggcattt ttttaagggg tggtaatgaa cgattataaa atgacgccag gtgagaggcg
     5701 cgcgacctgg ggtttaggga ccgtattctc gttgcgcatg ctgggcatgt ttatggttct
     5761 gccggttctg accacgtacg gcatggctct gcaaggtgcc agcgaagcat taatcggtat
     5821 tgccattggt atttatggtc tgactcaggc cgtttttcag attccgtttg gcctgctttc
     5881 cgaccgcatt ggtcgcaaac cattaattgt cggtgggctg gcggtgtttg ccgccggtag
     5941 cgttatcgct gcgctttccg actccatctg gggaattatt ctgggccggg cgctacaagg
     6001 ctctggagca attgccgccg ccgttatggc gctgctttcc gatctcacgc gcgaacaaaa
     6061 ccgcaccaaa gcgatggcgt ttatcggcgt gagctttggc attacctttg ccattgcgat
     6121 ggtgcttggc ccgatcatca ctcacaaact tgggctgcac gcgctgttct ggatgatcgc
     6181 tattctggca acaaccggca ttgcgttgac catttgggtt gtgcccaaca gtagcactca
     6241 cgtacttaat cgtgagtccg gaatggtgaa aggcagtttc agtaaagtgc tggcggaacc
     6301 gcggctgcta aaactcaact ttggcattat gtgtctgcac atgcttctga tgtctacgtt
     6361 tgttgccctg cccggacagc tagctgatgc ggggttcccg gcggctgaac actggaaggt
     6421 ctatctggcg acgatgttaa tcgcctttgg ctcggtcgtg cctttcatta tctacgctga
     6481 agttaagcgc aaaatgaagc aagtctttgt cttctgcgtc gggttgatcg tggttgcgga
     6541 aattgtgttg tggaacgcac aaacgcagtt ctggcaactg gtggtcggcg tgcagctttt
     6601 ctttgtggcg tttaatttga tggaagccct tctgccctca cttatcagta aagagtcgcc
     6661 agcaggttac aaaggtacgg cgatgggtgt ctactccacc agccagtttc ttggcgtggc
     6721 gattggcggt tcgctgggcg gctggattga cggcatgttt gacggtcagg gggtatttct
     6781 cgctggcgca atgctggccg cagtgtggct ggcagtcgcc agtaccatga aagaaccgcc
     6841 gtatgtcagc agtttgcgca ttgaaatccc ggcggacatt gccgcaaacg aagcgttaaa
     6901 agtgcgtttg ctggaaactg aaggcgtcaa agaagtgttg attgcagaag aagagcattc
     6961 agcttatgtg aaaatcgaca gcaaagtgac aaatcgcttt gaggttgaac aggcaattcg
     7021 tcaggcataa aaaacgcggc gaattgcctg ctgcgctacg cttaccaggc ctacaaagtt
     7081 cttgagttac aaaccgttgt aggccgaatc aggcgttcat gccgcatctg acatgaacaa
     7141 aacgcacatt gtcgcgatta atcgcggaag tttttgaact ggaacggctg gccgagatcg
     7201 ccaccacgta ccatcgccat gacagcctgc aaatcatcac gagatttgcc agtcacacgg
     7261 atctcatcgc cctgaatttg cgcctgcact ttcagtttgc tgtctttaat catcttgacg
     7321 attttcttct gggtcgcgct ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt
     7381 ttaccgctat gaacgatatt ttccggtaca tccagtgaac tgccttcaat gccgcgcttc
     7441 agcagcttgg cacgcagaat atccagcaac tgattgacct ggaagtcgga ctcgctcaac
     7501 actttgatgg ttttgctggc gtcgttcagc tcaaatgagg cttcaacgtt acggaagtca
     7561 aaacgggact ccacttcgcg actcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca
     7621 acttcagaga caatatcgaa agatggcatc ttttcctctc ccttcatttt tgatgcgaag
     7681 cataatacct gcaaagttaa ataaccaaca gtcctgcacc aggacgtatc ccttaattgt
     7741 gcatagtcgc aacagcatgc acaattaagg gatagcctta ttgaacaccc ggagtggttg
     7801 cgggtgagga ggaacaatga aaattaccgt attgggatgc ggtgccttag ggcaattatg
     7861 gcttacagca ctttgtaaac agggtcatga agttcagggc tggctgcgcg taccgcaacc
     7921 ttattgtagt gtgaatctgg ttgagacaga tggtgcgata tttaacgaat cgctgaccgc
     7981 caacgatccc gattttctcg ccaccagcga tctgctcctg gtgacgctga aagcatggca
     8041 ggtttccgat gccgtcaaaa gcctcgcatc cacactgcct gtagctacgc caatactgtt
     8101 aattcacaac ggcatgggca ccatcgaaga attgcaaaac attcagcagc cattactgat
     8161 gggcaccacc acccatgccg cccgccgcga cggcaatgtc attattcatg tggcaaacgg
     8221 aatcacgcat atcggtccgg cacggcaaca ggatggcgat tacagttatc tggcggatat
     8281 tttgcaaacc gtgttgcctg acgttgcctg gcataacaat attcgcgccg agctgtggcg
     8341 caagctggcc gtcaactgtg tgattaatcc actgaccgcc atctggaatt gcccgaatgg
     8401 tgaattacgt catcatccgc aagaaattat gcagatatgc gaagaagtcg cggcagtgat
     8461 cgagcgcgaa gggcatcata cttcagcaga agatttgcgt gattacgtga tgcaggtgat
     8521 tgatgccaca gcggaaaata tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac
     8581 cgaaatcgac tatatcaatg gttttctctt acgccgcgcc cgcgcgcatg ggattgccgt
     8641 accggaaaac acccgcctgt ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat
     8701 cggcactggt ttgcctcgcc cctggtagtg aagagactga agccgtcacc actatcgatc
     8761 tgctggttcg cggcggtatc aaagtcacca ctgccagcgt cgccagcgat ggcaacctgg
     8821 ccattacctg ctcgcgcggc gtgaagctgc tggcggatgc gccgctggtc gaggtggctg
     8881 atggcgaata tgacgtgatc gtactgcctg gtggcattaa aggcgcagag tgttttcgcg
     8941 atagtaccct gctggttgaa accgttaaac agttccaccg ttccgggcgt atcgtcgcgg
     9001 ctatttgcgc cgcgccagcc accgtgctgg tgccgcacga tatcttcccg attggtaata
     9061 tgaccggctt cccgacgctg aaagacaaaa ttcccgctga acaatggcag gacaagcgcg
     9121 tcgtctggga tgcacgggta aaattgctga ccagccaggg gccggggaca gctatcgact
     9181 ttggtctgaa aattatcgac ctgctggttg ggcgcgagaa agcccatgaa gtggcatcac
     9241 aactggtgat ggcggcaggg atttataatt attacgagta gtgtcggatg cggtaagcgt
     9301 cgcatctgac cagatgcgac gttacaacca caaattacgg gcgatatacc ttcacattgt
     9361 taaagccctg ctcgcgcaga tagagcgcct gcagacggct catcaccccg cgctcacacc
     9421 acagtagcca ggttctgttc tggtcgagat cgccaaactt ggtgctcagt ttatagaacg
     9481 gcagagaaac tacatcaatc ccttcgactt tcagtggctt atcttcctgt tcatcgatag
     9541 aacggatatc gaggatcacg tcgttcgggc cgaagccatt gacggtttcc acttccacca
     9601 cttcctgctc ggtctgctgg gcgatttcgc ggatatcaac gttattcgct tcttcaacca
     9661 ctttatcgag aatgctgaag tcgaatttct cttcttccgc ttcaatcttc gacttaaccg
     9721 ctttcaccgt cgggcttttg gagataacac cgcaatattc cggcatcgtg cgagcaaagt
     9781 cttcggtgcc aatctggcga gccaggttga tgatgtgctc tttgtcgtaa gaaatcagcg
     9841 gacgcaggat cagcgtatcg gagacgttat caatcaggcg caggttggtc agcgtctgac
     9901 tggacacctg gccgagcgct tcgccggtga ccagcgcctg tacgccgtaa cgttcagcca
     9961 ctttagacgc ggcacgcacc atcatacgtt tgaggataac gcccatctga ccgtcgtcga
    10021 ttttctcgag aatttccccg acgaccggtt cgaaattaat agcgacaaaa cgcacgcggt
    10081 gggagctgcc aaaacggttc cacagataat gcgccacctg acgcacgcca atttcatgcg
    10141 ccgcgccgcc aaggttaaag aaacagtaat gcacgcggca gccgcgacgc atcaacatat
    10201 aactggaaac accggagtcg aaaccaccgg aaatgagcga cagcacatct tcctgggtgc
    10261 cgatcgggaa accgccaata ccttcgtagc ggcctttaat cagcaggaga cgatcgtctt
    10321 ccacttccag atggacagtc acttccggat tggtcagctt cacgcgcgcg gactcaatat
    10381 gctgatttaa accgccgccg acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc
    10441 cacggcgctt cacgcgtacg cagaaggttt tgccttccag ctgatcgcga tactgaacca
    10501 acgctttctc aaaaatatcg tgcatgtcgg taaacggcac gtcttcgact tcgagaatat
    10561 ggtggatacc cggaatacgg gtcagggcgt cgcgaatagc cagacgctgg ttttcatctt
    10621 ttgcgcgaac ttcgatgtta tcccagtggc ggacgacagc gagcgtctca tcatagtgct
    10681 ttaaaacgtt acgaatgttc ccggtaagga tttttataaa gcgcaagcgc acagattggc
    10741 ttttgatggt gatttccggg aacaatttaa tgataaactt catggcggca atggttcgtt
    10801 ggcaagcctt aagcgacttg tatagggaaa aatacagcag cccacacctg cggctgcatc
    10861 caggcgcgga agtataccac taacatcgct ttgctgcgca catcacctta ccattgcgcg
    10921 ttatttgcta tttgccctga gtccgttacc atgacggggc gaaaaatatt gagagtcaga
    10981 cattcattat gccgaagaaa aatgaggctc ccgccagctt tgaaaaggcg ctgagcgagc
    11041 tggaacagat tgtaacccgt ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg
    11101 agttcgaacg cggcgtgcag ctggcacgtc aggggcaggc caaattacaa caagccgaac
    11161 agcgcgtaca aattctgctg tctgacaatg aagacgcctc tctaacccct tttacaccgg
    11221 acaatgagta atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc
    11281 gctgagccgt tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca
    11341 gtatggcgca ttattaggtg gtaagcgtct gcgacccttc ctggtttatg ccaccggtca
    11401 tatgtttggc gttagcacaa acacgctgga cgcacccgct gccgccgtag agtgtatcca
    11461 cgcttactca ttaattcatg atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg
    11521 tttgccgacc tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt
    11581 acaaacgctg gcgttctcga ttctaagcga tgccaatatg ccggaagtgt cggatcgcga
    11641 cagaatttcg atgatttctg aactggcgag cgccagcggt attgccggaa tgtgcggtgg
    11701 tcaggcatta gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat
    11761 tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc
    11821 cggagataaa gggcgtcgtg ctctgccagt actcgacaag tacgcagaga gcatcggcct
    11881 tgccttccag gttcaggatg acatcctgga tgtggtagga gatactgcaa cgttgggaaa
    11941 acgccagggt gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga
    12001 gcaagcccgg aagaaagccc gggatctgat cgacgatgcc cgccagtcgc tgaaacaact
    12061 ggctgaacaa tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg
    12121 taataaataa acaataagta ttaataggcc cctgatgagt tttgatattg ccaaataccc
    12181 gaccctggca ctggtcgact ccacccagga gttacgactg ttgccgaaag agagtttacc
    12241 gaaactctgc gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagcgggca
    12301 cttcgcctcc gggctgggca cggtcgaact gaccgtggcg ctgcactatg tctacaacac
    12361 cccgtttgac caattgattt gggatgtggg gcatcaggct tatccgcata aaattttgac
    12421 cggacgccgc gacaaaatcg gcaccatccg tcagaaaggc ggcctgcacc cgttcccgtg
    12481 gcgcggcgaa agcgaatatg acgtattaag cgtcgggcat tcatcaacct ccatcagtgc
    12541 cggaattggt attgcggttg ctgccgagaa agaaggcaaa aatcgccgca ccgtctgtgt
    12601 cattggcgat ggcgcgatta ccgctggcat ggcgtttgaa gcgatgaatc acgcgggcga
    12661 tatccgtcct gatatgctgg tggtcctcaa cgacaatgaa atgtcgattt ccgaaaatgt
    12721 cggcgcgctc aataaccatc tggcacagct gctttccggt aagctttact cttcgctgcg
    12781 cgaaggcggg aaaaaagttt tctctggcgt tccgccaatt aaagagctgc tcaaacgtac
    12841 cgaagaacat attaaaggca tggtagtgcc tggcacgttg tttgaagagc tgggctttaa
    12901 ctacatcggc ccggttgacg gtcacgatgt gctggggctt atcaccacgc tgaagaacat
    12961 gcgcgacctg aaaggcccgc agttcctgca tatcatgacc aaaaaaggtc gtggttatga
    13021 accggcagaa aaagacccca tcactttcca cgccgtgcct aaatttgatc cctccagcgg
    13081 ttgtttgccg aaaagtagcg gcggtttgcc gagctattca aaaatctttg gcgactggtt
    13141 gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt actccggcga tgcgtgaagg
    13201 ttccggcatg gtcgagtttt cacgtaaatt cccggatcgt tacttcgacg tggcaatcgc
    13261 cgagcaacac gcggtgacct ttgccgccgg tctggcgatt ggtgggtaca aacccattgt
    13321 cgcgatttac tccactttcc tgcaacgcgc ctatgatcag gtgctgcatg acgtggcgat
    13381 tcaaaagctc ccggtcctgt tcgccatcga ccgcgcgggc attgttggtg ctgacggtca
    13441 aacccatcag ggcgcttttg acctctctta cctgcgctgt ataccggaaa tggtcattat
    13501 gaccccgagc gatgaaaacg aatgtcgcca gatgctctat accggctatc actataacga
    13561 cggcccgtcc gcggtgcgct acccgcgcgg taacgcggtt ggcgtggaac tgacgccgct
    13621 ggaaaaactg ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggcgatcct
    13681 taactttggt acgctgatgc cagacgcggc gaaagtcgct gaatcgctga acgctacgct
    13741 ggtcgatatg cgttttgtga aaccgcttga tgaagcgtta attctggaaa tggccgccag
    13801 ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag gcagcggcgt
    13861 gaacgaagtg ctaatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgcctga
    13921 cttctttatt ccacaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc
    13981 cggtatggaa gccaaaatca aggcctggct ggcataatcc ctactccact cctgctatgc
    14041 ttaagaaatt attcatagac tctaaataat tcgagttgca ggaaggcggc aaacgagtga
    14101 agccccagga gcttacataa gtaagtgact ggggtgaacg aatgcagccg cagcacatgc
    14161 aacttgaagt atgacgagta tagcgggagt ggcagcatgc aatacaaccc cttaggaaaa
    14221 accgaccttc gcgtttcccg actttgcctc ggctgtatga cctttggcga gccagatcgc
    14281 ggtaatcacg catggacact gccggaagaa agcagccgtc ccataattaa acgtgcactg
    14341 gaaggcggca taaatttctt tgataccgcc aatagctatt ccgatggcag cagcgaagag
    14401 atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag acgtggtcgt tgcgacgaaa
    14461 gtgttccatc gcgttggtga tttaccggaa ggattatccc gtgcacaaat tttgcgctct
    14521 atcgacgaca gcctgcgccg tctcggcatg gattatgtcg atatcctgca aattcatcgc
    14581 tgggattaca acacgccgat cgaagagacg ctggaagccc tgaacgacgt ggtaaaagcc
    14641 gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt cgcagtttgc tcaggccctg
    14701 aagctacaaa aacagcacgg ctgggcgcag tttatcagta tgcaggatca ctacaatctg
    14761 atttatcgcg aagaagagcg tgagatgctg cctctgtgtt atcaggaggg cgtggcggtg
    14821 attccgtgga gcccgctggc gcgggggcga ctgacgcgtc cgtggggaga aactaccgca
    14881 cgactggtgt ctgatgaggt ggggagaaat ctctataaag aaagcgatga aaatgacgcg
    14941 cagatcgcag agcggttaac gggcgtcagt gaagaactcg gtgcaacacg agcacaagtt
    15001 gcgctggcct ggttgttgag taaaccgggc attgccgcac cgattatcgg tacatcgcgg
    15061 gaagaacagc ttgatgagct gctgaacgcg gtggatatca ctttgaagcc agaacagatt
    15121 gccgaactgg aaacgccgta taaaccgcat gcggtcgtag gatttaaata agagtccagg
    15181 cctgatgaga cgcaacaagc gtcacatcag gcactggtgc acaactacga cagaataccc
    15241 agcggccagt gatgaccgat aaagtacagg atacctgcgg agatcacccc ggcgacaata
    15301 tcatcgatca tgatccccat gccgccatgt acattgcgat caaaccagcg gatcggccac
    15361 ggcttccaca tatccagaat acggaaaatc acaaacccgg cggcaaccca ctgccagtca
    15421 ttggtcggca gcgccatgag cgtgatccac ataccaataa attcgtccca gacaatgctg
    15481 ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac aaagatagac gccgatacag
    15541 atccccaaca tcaccaccag cgagtagagc tgccagggta aaaaggtcat cagataccag
    15601 aacggaatcg ctgccagcga tcccatcgtg ccaggaacga ttgggcttaa tccacttccg
    15661 aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt cgcgacatct
    15721 ttatggcgtg gcaaaatggt catatccttt ccagtcaaac gtgacaggtt cgccgtcacg
    15781 aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa acggtacgcc
    15841 caggtgtccg agagccacat ccagcgcgcc acggttcagt tccggcacag tgaaacacaa
    15901 ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggtt caacatggcg
    15961 agaaagcgca tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc
    16021 tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag ctgaatttgc
    16081 cagatcgcgc agtgcctgtc cctgtaaaat acgcggcgat ggacggagat gacgtttgat
    16141 caagtagtcc gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc
    16201 ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt tcgctccaga
    16261 gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccaacgtca ttgataatgg
    16321 cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa gatcaaacaa
    16381 actgtcgctg aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta atgccagcgt
    16441 cagccaggcc ggatcggccc ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt
    16501 atacgccaga tcagcaggat cgatatcatg gaggaagtgg ttgcccgcca ccagcgtatc
    16561 agtgctgatc gccagggtct gtttctcggg gatattgaga agagcgcaat cgtcgccgat
    16621 gcccagttcg acatcaagac gagaacttct tacacggtca aaataacggg caatcaggga
    16681 gaactcgcca catgccatac gttatgcctc agcaggta
//
LOCUS       DAFATA010000099        16488 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFATA010000099 DAFATA010000000
VERSION     DAFATA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.100"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..2451
                     /gene="uvrA"
                     /locus_tag="LGJ20_003774"
     CDS             <1..2451
                     /gene="uvrA"
                     /locus_tag="LGJ20_003774"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK2387788.1"
                     /translation="VPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKGEHTKTLEN
                     LASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLAESFETALE
                     LSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGACPTCDGLG
                     VQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEAPWGSLSAN
                     VHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSAVREELAKF
                     ISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAGQRAKIAEK
                     ILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGVMYVLDEPS
                     IGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGVHGGEVVAE
                     GPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLKDVTLTLPV
                     GLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHFDKVIDIDQ
                     SPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRCEACQGDGV
                     IKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEAREFFDAVPAL
                     ARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYILDEPTTGLH
                     FADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEILVSGTPE
                     TVAECEASHTARFLKPML"
     gene            complement(2486..2842)
                     /locus_tag="LGJ20_003775"
     CDS             complement(2486..2842)
                     /locus_tag="LGJ20_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK2387789.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2846..3262)
                     /locus_tag="LGJ20_003776"
     CDS             complement(2846..3262)
                     /locus_tag="LGJ20_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBK2387790.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(3373..4086)
                     /gene="aphA"
                     /locus_tag="LGJ20_003777"
     CDS             complement(3373..4086)
                     /gene="aphA"
                     /locus_tag="LGJ20_003777"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBK2387791.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDIGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            4488..4991
                     /locus_tag="LGJ20_003778"
     CDS             4488..4991
                     /locus_tag="LGJ20_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387792.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICTLCCFAEEVKGDYS
                     HYRTFLFDIGNQYKVKLTQSGKKELSLTLEFNDTIIESQKVTGNKAKHILEDIEKFYR
                     NKPDTYY"
     gene            complement(5213..6406)
                     /gene="tyrB"
                     /locus_tag="LGJ20_003779"
     CDS             complement(5213..6406)
                     /gene="tyrB"
                     /locus_tag="LGJ20_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBK2387793.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(6659..7738)
                     /gene="alr"
                     /locus_tag="LGJ20_003780"
     CDS             complement(6659..7738)
                     /gene="alr"
                     /locus_tag="LGJ20_003780"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK2387794.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(7791..9206)
                     /gene="dnaB"
                     /locus_tag="LGJ20_003781"
     CDS             complement(7791..9206)
                     /gene="dnaB"
                     /locus_tag="LGJ20_003781"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK2387795.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            9289..10272
                     /locus_tag="LGJ20_003782"
     CDS             9289..10272
                     /locus_tag="LGJ20_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBK2387796.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGCKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(10438..10680)
                     /gene="pspG"
                     /locus_tag="LGJ20_003783"
     CDS             complement(10438..10680)
                     /gene="pspG"
                     /locus_tag="LGJ20_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBK2387797.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(10814..11851)
                     /gene="dusA"
                     /locus_tag="LGJ20_003784"
     CDS             complement(10814..11851)
                     /gene="dusA"
                     /locus_tag="LGJ20_003784"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBK2387798.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(12215..13205)
                     /locus_tag="LGJ20_003785"
                     /pseudo
     CDS             complement(12215..13205)
                     /locus_tag="LGJ20_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
     gene            13523..14038
                     /gene="zur"
                     /locus_tag="LGJ20_003786"
     CDS             13523..14038
                     /gene="zur"
                     /locus_tag="LGJ20_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBK2387799.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(14080..14289)
                     /locus_tag="LGJ20_003787"
     CDS             complement(14080..14289)
                     /locus_tag="LGJ20_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK2387800.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(14405..15730)
                     /gene="dinF"
                     /locus_tag="LGJ20_003788"
     CDS             complement(14405..15730)
                     /gene="dinF"
                     /locus_tag="LGJ20_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBK2387801.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(15803..16411)
                     /gene="lexA"
                     /locus_tag="LGJ20_003789"
     CDS             complement(15803..16411)
                     /gene="lexA"
                     /locus_tag="LGJ20_003789"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBK2387802.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
BASE COUNT         4294 a         4189 c         3966 g         4039 t
ORIGIN      
        1 gtcccgctgg cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa
       61 ggcaagcgtc tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa
      121 acgctggaga acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc
      181 gatctttccg atccgccgaa actggaactg caaaagaaac acactattga agtggtggtt
      241 gatcgcttca aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg
      301 ctggagcttt ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag
      361 ctgctgttct ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag
      421 ccgcgactgt tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc
      481 gtacagcaat atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc
      541 ggtgcgatcc gtggctggga tcgccgcaac ttctattatt tccagatgct gaaatcgctg
      601 gcagatcact ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat
      661 aaagtggtgt tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt
      721 ggcgatacct ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt
      781 tataaagaga cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc
      841 ccatgcgcca gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag
      901 aatacgccgc tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac
      961 aatctcaaac tagctggtca gcgggcgaag attgcagaaa aaatccttaa agagatcggc
     1021 gatcgtctga aattcctcgt taacgtcggc ctgaattacc tgacactttc ccgctcggca
     1081 gaaacacttt ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc
     1141 ctggttggcg ttatgtatgt actggacgag ccgtctatcg gcctgcacca gcgcgataac
     1201 gagcgcctgt tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg
     1261 gagcatgacg aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg
     1321 ggtgttcacg gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcagtgcct
     1381 gaatcgttga ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc
     1441 gttccggcga atccagaaaa agtgctgaag ctgacaggtg cacgcggcaa caacctgaag
     1501 gacgtgacgc tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc
     1561 ggtaaatcga cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt
     1621 gcgaccatcg ccgaaccggc accgtatcgc gatattcagg ggctggagca tttcgataaa
     1681 gtgatcgata tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat
     1741 accggcgtgt ttacacctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt
     1801 ggttatacgc caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag
     1861 ggcgacggtg tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat
     1921 cagtgcaaag gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc
     1981 atccacgaag tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca
     2041 gctctggcgc gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg
     2101 cagtccgcaa ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg
     2161 tcaaaacgcg gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac
     2221 ttcgccgata ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc
     2281 attgtggtga ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg
     2341 ggaccagaag gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc
     2401 gcggagtgcg aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg
     2461 ccgctttctg agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg
     2521 gtagtaaatt cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg
     2581 gcagcgaacc gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac
     2641 gcacatcgct gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa
     2701 cagctgggcg attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct
     2761 gcgtcgcttt ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt
     2821 gtagcaactc cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg
     2881 atccgccgtg gatgcgatgt tcccccagcc agatgccttg ccaggtgccg gtctgaatac
     2941 gccctttatg taacggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag
     3001 gcatatcgtc tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga
     3061 ggaaaaaacg ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag
     3121 aggcggaggt atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag
     3181 ccagctgatt cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg
     3241 tgagcgtctt ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca
     3301 gcaagacagc gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcacgc
     3361 tctcccgctc tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg
     3421 tggcaagggt ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgat
     3481 atcgcgtgcg gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc
     3541 ctgcagccat tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttactggatt
     3601 catgttggtg gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt
     3661 cgtcgggcta cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat
     3721 cagctggcga gcgacctctt ttggaatgct gaattcatcc cagccattgt tcattttttc
     3781 ccagaacaca ggatttttca gataatcttc gctttctggt gagaaggttt ttttgccgcg
     3841 ccagaagccc gggctggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg
     3901 cggacgccct gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg
     3961 ttcagcaagc ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc
     4021 aacagcggaa ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt
     4081 gcgcatattt ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca
     4141 aaaaccatct gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt
     4201 gatgaaaagc aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata
     4261 aaaaatgttt attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct
     4321 cggcaaatga gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg
     4381 atacagcatt acaaaaacaa tcgaagttta taaagatgat tcctgattga ccagaaccct
     4441 atcctacaat ggtgactaat tttattactc ccgatggaga tgatgatatg aatatatcat
     4501 atgtgaatag caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag
     4561 atatctctta tgccaaagat ttttcttatg ctaaagattt ctttttatac atagaaacac
     4621 agttaaaaat tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa
     4681 gtaaagtttt tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta
     4741 tgtatatttg cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca
     4801 ggaccttttt atttgatatt ggtaatcaat ataaggttaa acttacacaa agcggaaaaa
     4861 aagagctctc tttaacttta gaatttaacg atactataat tgaatctcag aaagtcacag
     4921 gcaataaggc aaagcatatt cttgaggata tagaaaaatt ctatcgtaac aaacccgata
     4981 cttattatta aatttaataa tagttagatg tgcagaaatt attactccac ccaaaaaaac
     5041 aggataagga attccagata attaggataa gcttaacaat aaaagaacta ataacgttta
     5101 ttatctctca cgtagaacga tggcatcaaa aaaatcatct ataaagaaag agtgaaagcg
     5161 acgacagttt aatttcactg caggctgggt agctccagcc tgctttcctg cattacatca
     5221 ccgcagcaaa cgcctttgcc acacgttgta catttgccgt atttaacccg gcgacacaca
     5281 tgcgaccgct ggcgatgaga tagacaccaa attcttcacg tagtcggtca acctgagcgg
     5341 cacttaaacc ggtataactg aacatgccgc gctgattaag cagataatcg aaattgcgtt
     5401 ctggcatctc tgtgcttaat accttcacca gttcctgacg cattgccaga atgcgagtac
     5461 gcatctcttc tacttccgcc agccagctgg ctttcaatgc ctcgtcattc agcactgcag
     5521 ccaccacctg cgcaccaaaa ttcggcgggc tggagtagtt gcggcgaact gttgctttca
     5581 attgccccag tacgcggcca gcggcttcgg catcttcaca cagaacagaa agtccgccga
     5641 cgcgctcgcc gtaaagggag aaaattttcg agaacgaatt gctcaccaga gcgggtaatc
     5701 cagcgctggc aatggcgcga atagcgtagg catcctcttc cataccggca ccaaatcctt
     5761 gataggcaat atcgaggaat ggaataagct cgcgggattt gagaatttca atcaccgcat
     5821 cccactgatc attagtgaga tcggcacccg ttgggttgtg gcaacatgga tgcagcaaca
     5881 caatactgcg ggcaggtaat gttttcagcg tcgccaacag gtcattaaag cgcacgccgt
     5941 tagtcgcttc gtcataccag gggtaagtac tcacttcgaa tccagccccg gcgaatattg
     6001 ctacgtggtt ttcccaggta ggatcgctga cccagacgcc tgattccggg aagtagcgtt
     6061 tcaggaaatc cgcgcccact ttcaatgccc ctgagccgcc aagggtttga atggttgcta
     6121 cgcgctgttg ttgcagtact ggatggtccg caccaaacag cagcggcgca atggcatggc
     6181 gatagctgtt aagcccttcc atcggtaaat aaagcgaagc gccatgaggc tgcgcattca
     6241 ggcgcgcttc cgcctccgcc acggcttgca gttgtggaat aattccgtct tcgttgtagt
     6301 acagaccgat acttaaattc actttgtcgc tgcgagggtc ttctttaaaa cgctccataa
     6361 gcgtaagaat cgggtcgcca gcgtaggcgt caactttttg aaacacgcga tggttctcca
     6421 ggtttacggg caggtggtta aaacacaata aaccggaaga aggcgaagat cgagtggatg
     6481 ttcaggagcg aacggcaatt agcaacagag tgagactcat gacaaacgta catccgccag
     6541 aggcacgacc tttataagag catgaaagaa catcaactta ttgaattttt aggattttat
     6601 tggccggata aggcattcac gccgcatccg gcacagacaa tcaaatatta cagaacgatt
     6661 aatccacgta tttcatcgcg acccttgaag tcaggcgcgt aataagttcg taagcgctta
     6721 ctttcgtcat ttcagcgata cgttctacgg gcaaaccttc gccccataaa atgaccggat
     6781 ccccggcttt gtcctgcgct tgtggaccta agtctacgca gatcatatcc atcgccacgc
     6841 gcccgacaat cggtacttcg cgaccgttca ccagcactgg cgtaccggac ggcgcggcgc
     6901 gcggataacc atcgccatag cccatcgcga ctacgccaag acgggtatca cgttcgctta
     6961 cccaggttcc accataacca acaggttctc cggctttatg ctcacgcacg gcaatcaggc
     7021 tggaggttag tgacatcact ggctgacagc caaaatcggc accggtggag cgatcttcca
     7081 gcggcgagac gccataaaga atgatgcccg ggcgcaccca gtcaaaatgc gactgtggcc
     7141 acagcagaat gccacccgat gcggcaatgg aacgttgacc aggtttgcct tcgcaaaagg
     7201 tattaaagat agcgagttgt ttctcggttg cgccacattt tggttcatcc gcgcgcgcaa
     7261 aatggctgac gatattcacc ggctgacgaa cgtttttgca ctgggtcagg cgatgataaa
     7321 acgcctcagc ctgttccggc cttacgccca gacggtgcat accggtatcg agtttcatcc
     7381 agacggtaac cggctcgtcc aggctagcct cttccagcgc agccagctgt tcttcgttat
     7441 gcacggcggt atgaaaatgt tgcgcagaaa tcgtcggcag atctctggca tcaaaaaagc
     7501 cttcgagtaa cagtacaggt ttggtgattc cccccgcacg cagtcgcaga gcttcttcga
     7561 gacgggctac gccaaaggcg tcagcatcgg ggagcgttcg cgcggtctca agaagaccgt
     7621 gaccataagc gttcgctttc accaccgcaa ccattttact ggcaggggcc agttcacgaa
     7681 gacgttgcag gttgtgtcgc agagcgcggc ggttaatcac aacagttgcc gcttgcattt
     7741 gtgttccttg ataagtgttt gctttaatta cctaattcat aaaataatta ttattcgtcg
     7801 tcgtactgcg gccccgcata gttgtcgaag cgcgaccatt gaccgttaaa ggtcaggcgt
     7861 accgtcccga ttgggccgtt acgttgttta ccgataataa tttccgcgat gccttttaaa
     7921 tcactgtttt cgtgatacac ctcatcacga tagataaaca tgatcaagtc cgcatcctgc
     7981 tcgatagagc cagattcacg caggtcggag ttgaccgggc gtttgtcggc acgttgttcc
     8041 agagaacggt tcaactggga cagcgccacc accggcacgt tcagttcttt cgccagtgct
     8101 ttcagcgagc gggagatttc tgcaatttcc agcgtacggt tatcggaaag cgccggtacg
     8161 cgcatcagtt gcaggtagtc gatcatgata agcccgatgc cgccgtgttc acgggcaata
     8221 cggcgtgcgc gggaacgcac ttccgttggc gtcaggccgg aggagtcatc gatatagata
     8281 ttgcgttttt cgagcaaaat acccatggtg ccggaaatgc gcgcccagtc ttcatcatcg
     8341 agctgcccgg tacggatttt agtctggtca acgcgcgaca gcgacgccag agaacgcatc
     8401 ataatctgtt ctgaaggcat ctccagcgag aagataagta ccggtttatc ctgcaacatc
     8461 gccgcgtttt cgacgaggtt catcgcaaat gttgttttac ccatcgacgg acgcgcggcg
     8521 acgatgatca aatccgacgg ctgcaagcca gcggtttttt tgttgagatc gtcataaccg
     8581 gtgtttactc cggtaacgcc atcgtgtggc tgctgaaaca actgctcaat acgcgccacg
     8641 gttgcgtcga gcacatcggc gatgttcttc ggcccttcgt ctttgtttgc acgactttcg
     8701 gcaattttaa agacgcggga ttcagcaagg tccagcagat cttcgctggt acgcccctgc
     8761 ggatcaaaac cggcttcagc aatctcattc gcaaccgaga tcatctcacg gacaacggca
     8821 cgttcacgca cgatgtccgc ataggcactg atgttcgccg cacttggcgt attttttgac
     8881 agctctgcca gataagcaaa accaccgacg ctatcgagtt gcccctggcg ttccagcgat
     8941 tccgcaaggg taatcagatc gataggacta ccgctttcct gcaaacgcgc catttcagta
     9001 aagatatggc ggtgtgggcg ggtgtaaaaa tcgtctgcca ccacacgctc ggctacatcg
     9061 tcccagcgtt cgttatccag cattaaaccg cccaacaccg actgctccgc ttcgatcgag
     9121 tgcggaggca ctttcagccc ggcaacttgt ggatcgcgtt cgcggggttc agcctgctgt
     9181 ttgttgaagg gtttatttcc tgccatagtg aatggagtta ccgagataaa gaatgggtcg
     9241 aaactttacc atatgaagca gaccctgacg atacgttctg gaggacacat ggcaacacga
     9301 attgaatttc acaagcacgg tggcccggaa gtacttcaag ccgtagagtt cactcctgcc
     9361 gatccggcag agaatgaaat ccaggtcgaa aataaagcca tcggcatcaa ttttatcgac
     9421 acgtatatcc gcagcggcct ttacccgccg ccatcactac ccagcggatt aggtaccgaa
     9481 gcggcaggca tcgtgagtaa agtcggcagt ggtgtaaagc atattaaggc aggcgatcgt
     9541 gtagtctatg cgcagtcggc gttaggcgct tacagctctg tgcataacat taatgcggat
     9601 aaagcggcga ttctgcctgc ggcaatttct tttgaacaag ctgcagcatc tttcctgaaa
     9661 ggcttaacgg tttattatct gctgcgcaaa acctatgaaa ttaaacccga tgaacagttc
     9721 ctgttccacg cagcggctgg cggcgttggc ttaattgcct gccagtgggc aaaagccctg
     9781 ggcgcgaaac ttatcggcac cgtaggaacc gcgcaaaaag cgcagagcgc gctaaaagcc
     9841 ggcgcgtggc aggttattaa ctatcgtgaa gagaatctgg tcgagcgatt gaaagagatc
     9901 accggctgta agaaagtgcg cgtagtgtac gattccgtgg gcagagacac ctgggaacgg
     9961 tcgctggatt gcctgcaacg ccgcggctta atggtcagtt ttggcaactc atcaggtgcg
    10021 gttaccggtg tgaacttagg cattctcaat caaaaaggct cgttgtatgt gacacgccct
    10081 tccctgcaag gctatatcac cacgcgggag gaattaaccg aggccagtaa tgaactgttc
    10141 tctttgattg ccagcggtgt gattaaggtc gatgtcgccg agcagcagaa atatccgctg
    10201 aaggatgcgc agcgtgcgca tgagattctg gaaagccggg cgacgcaagg ttccagcctg
    10261 ctgattccat aaaagaaata gggcttccac ctgggaagcc ctttcttttt atagttcggc
    10321 tgtatgtagg gtacagcacg atgaatctgt tagaggcgca atagtgacag atttgattat
    10381 caattcctat tttgttctaa ggataaaacc ttaggttgtg atcatccgca caatccctta
    10441 gtaacgccag cggtcataac gctgatattt cggcactttt ggtgctttaa tcgccttaat
    10501 aacccacacc accgcaatcg ccagtagtaa ccacggcagc aacttaatca tcaatgccag
    10561 cataccgccg aggaacataa tggccgtcgc cacaaccagc gcggcgataa tgcccagcaa
    10621 cgaaacgccg gtgaccatca gcatgacaaa aaagccaaac acaaaaagta gttccagcat
    10681 gatgctctcc caaatatgaa atctcttgct ggcattacaa gaatcatgcc aaaaataatc
    10741 tattgattta acagcaaaac gccccgcgac ggtgcgcagg gcgtggtgaa tttgactact
    10801 ttttggtgaa aagttaacgc ttatccgcca ccagtttgag cgcgtgttcc agcacattaa
    10861 tgtctgcacc cgctttatgg gcattttcac ttaaataacg ccgccactgc cgcgcgccag
    10921 gaataccctg gaacaagccc aacatatgcc gggtaatatg gccgagatac gtcccctggc
    10981 tgagttcacg ctcaatgtac ggatacatgg cgcgcactac cgccaccgga tcggcatcgg
    11041 tatccgagga accaaagatc tcccggtcta ccgccgccag aatacccgga ttctgatacg
    11101 cctcgcgccc gaccatcacg ccatccatat gttgcaggtg tgctttggcc tcttccagcg
    11161 acttgatacc accgttaatc gacattgtca gatgcggaaa gtcacgcttc agttgataca
    11221 cacgcggata atcgagcggc gggatttcac ggttttcttt cgggcttaac cccgaaagcc
    11281 aggctttacg tgcgtggatg ataaacatct cacactcgcc tttgccggaa acggtgttga
    11341 tgaaatcgca gagaaattca tagctgtcct ggtcatcgat gccaatacgc gttttcaccg
    11401 tcactggaat cgacaccaca tcgcgcatcg ctttcacgca gtcggcaacc agctgcgcat
    11461 tacccatcag acacgcacca aacatgccgt tctgcacccg gtcagacggg cagccgacat
    11521 tcaggttgat ctcatcatat ccgcgcgctt ctgccagctt tgcacactgc gccagcgccg
    11581 ccggatcgct accgccgagt tgcaacgcta ccggatgttc ttcttcactg tacgccaggt
    11641 aatcaccttt accgtgaata atcgcccctg tggtcaccat ttcggtatac agcaacgtat
    11701 tgcgggaaag cagacgcaag aaatagcggc aatgtctgtc cgtccagtcg agcattggtg
    11761 caacgctaaa ccgaccactc cagtgaacgt cagttttttc aggcattgcg ctggtttggt
    11821 tgattttttg catttcagaa ttaccgtgca ttttcaaatg tagagattat tttatcgata
    11881 tatcattggg ttatgttatt cagcatcact gttcaggagg ctcaatagcg gggtactata
    11941 ccataacaac aggaagcgcc tgtctcattg caaaagaaaa ttgagatcaa atcaaggcat
    12001 gaagctctca cgaagtgatg gaaataatct tattagccgt tagccttgtt aaggacaatg
    12061 ataaacaatc caggttcgac gataaataaa aaatcacaca ttaaactccg gtgatatctc
    12121 ttcctgctaa tggcactgat agaagaaaaa agaacccaat aagtattagg ttcttttatg
    12181 taatgccttc cataccatcg aagaacttca catattattt cgccgattta accccgaaca
    12241 aatcataaat taaattagaa gtatctgtaa gtattttaat cttttccttt gagtttgggt
    12301 caaacgactt agcaaagtca attaatttcg gtgcagcatc tcgcattttg cttaaaatat
    12361 caggttcgag ctttccttca ttcaccagaa gtgacatttt atccagatag tcatcaaatt
    12421 tcatcccccc cggtccgcca gatgcttcat ctctcccagg tacttcttca tatcacgttg
    12481 agataattct tcaaactgag ctgtcagata gttctcatta tattcattaa tattcacttt
    12541 ttctgctccg gctaaatctg tgtaggcact agaatatgaa attgaaatta acagcgaagc
    12601 aaataatttt aagctgttgt catcatgttc ctggcaggca ttaacaaacg ttaaaaaacc
    12661 agaacctatt gcttgaaaat gtatattcgc taaaggttca ttatccttag attcctcata
    12721 aaatagctga accgtggagg gaaggccatt cttagtttct acatcaaaag tacatcgttc
    12781 aatgggagtc gcatctttat tatccccagg attacatata ctattaatcg cgaaatgaaa
    12841 aatagcccgg tcaacaactg atgcaataaa gtcacgatcg tttaaatcct catcagtgca
    12901 atcgtcaata tacttattga cccacatttt gagatcattt atttgctcaa ttaaaaacac
    12961 atttttttac gtgcatgcca taaattatgc gtgattcgtt gaataaacaa atctattttt
    13021 gcaataatta tatcccgatg acgcagcgct gcataatcaa tagatttacc atatatatta
    13081 tttaatttac ctttgattga acctctatta tctaatttat ctttgattgc atcttcatta
    13141 tcgaattgat ttgaattgag tgttttatta aagtatccag ttgcaccggg tattatcttc
    13201 aacatgattc atctaccttt tttgtcgtac cgtgtcctcc ttataattct tttgccatct
    13261 ggtcactatc aaaaatcgct cataatttct gaactacagg tatgcgttta aacccagtta
    13321 aataaatagc gagttaactt gaagcaattt atttaaaaat gaattattaa ttattatcgt
    13381 gttgataatg gtacgacaag gcaacacaac accttaccta caaaacagaa aatcaacatt
    13441 acggcaacaa taagggttct cggtgttttt cctggaacat ggtaaagtaa ggacattctt
    13501 aacccccact ttgaggtgcc cgatggaaaa gaccacaacg caggagttat tagcgcaggc
    13561 tgaaaaaatc tgcgcgcagc gtaatgtgcg cctgacccca cagcgcctgg aagtgttgcg
    13621 cctgatgagt ctgcaggatg gcgctatcag cgcttatgat ctgcttgatt tactgcgcga
    13681 agctgaaccg caagccaagc cgccaacggt ttatcgcgcg ctggattttc tgcttgagca
    13741 aggttttgtg cataaggtgg aatccaccaa cagttatgtg ctctgtcatc tgttcgacca
    13801 gcccacgcat acgtcagcca tgtttatatg cgatcgctgc ggcgcggtga aagaagagtg
    13861 tgcagaaggc gtggaagaca ttatgcatac gctggcggca aaaatggggt ttgccctgcg
    13921 gcataatgtg attgaagcac atgggctctg tgcggcatgc gtggaagtgg aagcgtgtcg
    13981 tcatcctgaa cagtgccagc atgatcactc tgtgcaggtg aaaaagaaac cgcgttaaga
    14041 gggcgtacat ccttgtacac gtcgggcagg agggattaat taccagcgat attcattgcg
    14101 ggtttcccaa tccacgacct ctttttctgc ctgatctttc tgataaccat aacgttcctg
    14161 gattttaccg accagttgat cacgtttacc ttcaatgatc gtcatatcat catcggtcag
    14221 tttgccccat tgctctttca ctttaccttt aaactgtttc cagttaccgc cggcttcatc
    14281 tttattcata atcaagacct catcgttagg ttgtgaatga gagtacgttc acttttcttc
    14341 tgaacgtgag attaagtgta gtcagcaatt tggcttagga taatttattc agaattttta
    14401 accgtcacgt tgcggcaaac caggtaccgt tgcgccagtg acgccgccag atagccgcca
    14461 gagaaagccc gcgcaacgcc agaaagacgg ttaatgccag ccacaaacca tgattaccca
    14521 gccacggcag cgtaaggagc gtcagcgcaa aacctgcggc ggccaccgcc atactgttac
    14581 gcatttcggc ggcacgcgtt gcgcctataa acatgccgtc cagcagataa caccagacgc
    14641 caaccaacgg caaaatcacc tgccagataa gatagcggtc agccagctgc tgaatctggg
    14701 ttaacgacgt cagcaacgca atgatgtgtt cccctgccag caaataaacc accgaaaaca
    14761 gtaacgctac aatccccgac tggcggcacg ctgcccgcca tacatccagc aactggctac
    14821 cgtcgcgcgc accgtatgcc tgaccggagt gcgcttcaac cgcgtaggca aaaccatcaa
    14881 gcgcataggc ggtaaaggtg agtagcgtca tcagaaccgc gttaacagcg acaatgtcac
    14941 tccccagtcg cgcaccaagt acggtgatcg cgccgaaaca gagttgcaac aacagcgaac
    15001 gcagcatgat atcgcggtta agcgccagca agcgacggaa gtttcctcgc caggcagttt
    15061 tcagcatttc gccggaaatt ccgcgtagtt tgaggatttt acgcaccatt agcagaccaa
    15121 tcagcaatgt tgcatattcc gcaataaccg tcgccagcgc cgcgccctgc acgttcatat
    15181 gcagccccat caccagccag acatccagca caatgttgag gatattgccg accactaaca
    15241 aaattactgg cgcacgggca tattgcacgc cgagtaacca accgagtaat accagattcg
    15301 ccagcgacgc cggagcgctt aaccagcgga tttcaagaaa gcgccgcgcc tgttccagga
    15361 ccgcttcact accgccaaca atatgcagcg ccagatcgat aatcggtgta cgcagcagcg
    15421 caattaacgc cccagccccc aacgccaaca gcaacggttg caccagcgca cgggctaatg
    15481 cctgaggatt tttggcacca taagcctgcg cagtcagccc ggtggtgctc atgcgtaaaa
    15541 acagcaacag cataaagaga aagctggtcg ccgttgcacc aaccgccacg ccgcccaaat
    15601 aaaccgggct atcaagatga ccaattaccg ccgtatcgac cagtcccagc aacggaacgg
    15661 tgatattgga gaaaatcatg ggtaaggcga gatgccagag tgctttatca gatgaagtga
    15721 ggaatgccat gcagacaagc ctgatgaaga gagatgaaaa acaaaccgcg acgcctggcg
    15781 gcatcgcggt ctcagagata tgttacagcc agtcgccgtt gcgaataacc ccaaccgcca
    15841 gcccttcaat ggtgaagctc tgctgacgaa ggtcaacgac aattggttta aactcgctat
    15901 tttctggcaa cagttcgact ttattgccct gttttttcag gcgcttaacg gtaacttcgt
    15961 catcaatacg tgcgacaacg acctgaccgt tacgtacatc ctgagtttta tgcactgcca
    16021 gcaagtcacc atccataatg ccgatatctt tcatcgacat cccgctgacg cgcagcagga
    16081 aatcagcatt cggcttgaac aaggaaggat cgacctgata atgaccttca atatgctgtt
    16141 gcgccagaag cggttcaccg gcagccacac gacctaccag cggcaaccct tcttcctctt
    16201 cctgcaacag acgaatcccg cgtgatgcgc cggaaacaat ttcaataacg cctttgcgtg
    16261 ccagcgcctt cagatgttct tcagccgcgt ttggggaacg gaaccccaaa cgctgcgcga
    16321 tttccgcacg cgtcggcggc atacctgtct ggctgatgtg atcacggatg agatcaaaca
    16381 cctcttgttg cctggccgtt aacgctttca ttccgccccc tgggtgtata tacagttatg
    16441 ctgtgagtat atacagcaaa aggcgatttt ggaaccataa actgcaca
//
LOCUS       DAFATA010000100        16371 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFATA010000100 DAFATA010000000
VERSION     DAFATA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.101"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..636
                     /locus_tag="LGJ20_003790"
     CDS             <1..636
                     /locus_tag="LGJ20_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK2387803.1"
                     /translation="KVLVDGAQAVMHHPVDVQALDCDFYVFSGHKLYGPTGIGILYVK
                     EALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEAGTPNTGGIIGLGAALEYVSAL
                     GLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGVIAFNLGKHHAYDVGSFLDNYG
                     IAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVDRLVTGLQRIHRLLG"
     gene            649..1065
                     /gene="sufE"
                     /locus_tag="LGJ20_003791"
     CDS             649..1065
                     /gene="sufE"
                     /locus_tag="LGJ20_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBK2387804.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            1214..2218
                     /gene="ldtE"
                     /locus_tag="LGJ20_003792"
     CDS             1214..2218
                     /gene="ldtE"
                     /locus_tag="LGJ20_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBK2387805.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(2282..2518)
                     /gene="lpp"
                     /locus_tag="LGJ20_003793"
     CDS             complement(2282..2518)
                     /gene="lpp"
                     /locus_tag="LGJ20_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBK2387806.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(2829..4241)
                     /gene="pykF"
                     /locus_tag="LGJ20_003794"
     CDS             complement(2829..4241)
                     /gene="pykF"
                     /locus_tag="LGJ20_003794"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBK2387807.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            4798..5007
                     /gene="fumD"
                     /locus_tag="LGJ20_003795"
     CDS             4798..5007
                     /gene="fumD"
                     /locus_tag="LGJ20_003795"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBK2387808.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            5463..6089
                     /locus_tag="LGJ20_003796"
     CDS             5463..6089
                     /locus_tag="LGJ20_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBK2387809.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            6110..8212
                     /locus_tag="LGJ20_003797"
     CDS             6110..8212
                     /locus_tag="LGJ20_003797"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBK2387810.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            8225..8863
                     /locus_tag="LGJ20_003798"
     CDS             8225..8863
                     /locus_tag="LGJ20_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBK2387811.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            8927..9595
                     /locus_tag="LGJ20_003799"
     CDS             8927..9595
                     /locus_tag="LGJ20_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBK2387812.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            9592..10377
                     /locus_tag="LGJ20_003800"
     CDS             9592..10377
                     /locus_tag="LGJ20_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBK2387813.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            10381..11193
                     /locus_tag="LGJ20_003801"
     CDS             10381..11193
                     /locus_tag="LGJ20_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387814.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(11205..12809)
                     /locus_tag="LGJ20_003802"
     CDS             complement(11205..12809)
                     /locus_tag="LGJ20_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBK2387815.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(12935..13240)
                     /locus_tag="LGJ20_003803"
     CDS             complement(12935..13240)
                     /locus_tag="LGJ20_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBK2387816.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(13348..13424)
                     /locus_tag="LGJ20_003804"
     tRNA            complement(13348..13424)
                     /locus_tag="LGJ20_003804"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13388..13390),aa:Val,seq:gac)
     gene            complement(13429..13505)
                     /locus_tag="LGJ20_003805"
     tRNA            complement(13429..13505)
                     /locus_tag="LGJ20_003805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13469..13471),aa:Val,seq:gac)
     gene            13813..15069
                     /locus_tag="LGJ20_003806"
     CDS             13813..15069
                     /locus_tag="LGJ20_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBK2387817.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(15110..>16371)
                     /gene="mdtK"
                     /locus_tag="LGJ20_003807"
     CDS             complement(15110..>16371)
                     /gene="mdtK"
                     /locus_tag="LGJ20_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBK2387818.1"
                     /translation="YSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRE
                     RIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPG
                     YLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATA
                     AVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLF
                     AVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTA
                     ARTGLMVGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQV
                     IGSGILRGYKDTRSIFYITFTAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLT
                     SAAIMMMLRMRFLQRLPSVIILQRASR"
BASE COUNT         4255 a         3729 c         4235 g         4152 t
ORIGIN      
        1 aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg
       61 gattgcgact tttacgtgtt ctccgggcat aaactgtatg gccccaccgg aattggcatt
      121 ctttatgtga aagaagcctt gttgcaggag atgccgccgt gggaaggggg cggttctatg
      181 atcgccaccg tcagcctgag tgaaggcact acctggacca aagcaccatg gcggtttgaa
      241 gccggtacac ccaataccgg gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg
      301 gcgctggggc ttaatagcat agccgagtat gaacagaatc tgatgcatta tgcgctatca
      361 cagctggaat ctgtaccgga tctcactctc tatggcccac aaaacaggct tggcgttatt
      421 gcttttaatc tcggtaaaca ccacgcctat gatgttggca gttttctcga taattacggc
      481 attgctgtgc gtaccggaca tcactgcgct atgccattaa tggcctatta caacgtccct
      541 gcgatgtgtc gggcgtcgct ggccatgtat aacacccatg aagaagtgga tcgtctggtg
      601 accggactgc aacgtattca ccgtctgctg ggataacagg gaggcactat ggctttattg
      661 ccggataaag aaaaattgct gcgtaatttt ttacgctgcg ccaattggga agagaaatat
      721 ctctacatta ttgagctggg ccagcgtctg ccagaattac gcgacgaaga ccgaagccca
      781 caaaatagca ttcagggctg ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag
      841 ggaattattg aattgcaggg cgacagcgat gcggcgattg tgaaagggct tattgcggtc
      901 gtatttattc tctacgatca gatgacgccg caggatattg tcaatttcga tgtgcgtccg
      961 tggtttgaaa aaatggcgct cacccaacat ctcaccccat ctcgttcaca aggtctggaa
     1021 gcgatgattc gcgcaattcg cgccaaagcc gctgcactta gctaaactaa agggacagct
     1081 ttcatccggt tggctcacat tgccggatgg tcaatgttta cactgacctg cacaggagtg
     1141 ttttggtttc aggtgaacat aagcgccatc cgacgtttca gcgtgagtct ccggcaaata
     1201 caggaggttt acaatgaaac gcgcgtcttt gcttacactc acgcttatcg gcgcttttag
     1261 cgccatccag gctgcctggg cggttgatta tccgctacca ccaaccggaa gccgactggt
     1321 tgggcaaaat caaacgtata cggtgcaaga aggggataaa aaccttcagg ctattgcccg
     1381 acgttttgat actgcggcaa tgttgatcct tgaagccaat aacactatcg ccccggtgcc
     1441 aaaacctggt acgacgataa ctattccttc acaactgtta ttacctgatg caccgcgtca
     1501 ggggattatc gttaaccttg cagagctgcg cctttattat tatccgccgg gagaaaatat
     1561 tgtgcaggtt tatccaatag gtattggatt gcaggggctg gaaacgccgg tgatggaaac
     1621 gcgtgttggg cagaaaatcc ctaacccaac ctggacgcct acggcaggca ttcgtcaacg
     1681 ttcgctggag cgtggcatta aattaccgcc agtcgttcct gccggaccaa ataacccgct
     1741 aggacgttac gcactgcgcc tcgcgcatgg taatggcgaa tacctcattc atggtaccag
     1801 tgcgccggac agcgtcggtt tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga
     1861 tattaaagcc ttgttctcca gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc
     1921 ggtgaaatat tccgtggaac ctaacgggat gcgttatgtt gaagtacatc gaccactatc
     1981 agcagaagaa cagcaaaacg ttcagacaat gccatacaca ctgccagcag gctttacgca
     2041 atttaaagac aataaggctg tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc
     2101 agggtatccg gtttcggtga gcagtggagc aactcccgca gccagcaatg cgccttcagt
     2161 agagtcagcg cagaatggtg aaccagagca agggaatatg ttacgcgcga cgcagtagcg
     2221 gtaaacggca gacaaaaaaa atggcgcaca atgtgcgcca tttttcactt cacaggtact
     2281 attacttgcg gtatttagta gccatgttgt ccagacgctg gttagcacga gctgcgtcat
     2341 ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc gttgctcagc tggtcaactt
     2401 tagcgttcag agtctgaacg tcagaagaca gctgatcgat tttagcgttg ctggagcaac
     2461 ctgccagcag agtagaaccc aggattaccg cgcccagtac cagtttagta gctttcatta
     2521 ttaataccct ctagattgag ttaatctcca tgtagcgtta caagtattac acaaagtttt
     2581 ttatgttgag aatatttttt tgatgggaag gcacttattt ttgatcgttc gctcaaagaa
     2641 gcatcgaacg ctgggtttta gataaaaaaa ttgagagaaa acaggatgct tccatcggat
     2701 tcatcttaga taaagtgaga ttatatagtg aaatccgatt tgatttttta ttgcttctgg
     2761 ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg atatacaaat taattcacaa
     2821 aagcaatatt acaggacgtg aacagatgcg gtgttagtag tgccgctcgg taccagtgca
     2881 ccagaaacca taactacaac gtcacctttg tgtgccagac cgctctgcag agccagttct
     2941 ttacccagac ggtagaaatc atcagtagaa gtgatctctt taaccagctg cggcacaacg
     3001 cctttgctca gtaccaactg atgagccgtt ttttcgttgg tggtcagtgc caggatggtg
     3061 gcatccggga agtatttacg tactgcgcga gcagatttac cgccctgagt agcaaccacg
     3121 atcagcggag catccagttt ttcagcagtt tcaacggcac cacggcatac cgcttcggta
     3181 atgcgcagtt tacggttgtc attgttgaac tcgagacggc tgttcatcac gcggtcggta
     3241 cgttcgcaaa tggtcgccat gatagaaacc gcttccagcg ggtatttacc ttttgcggat
     3301 tcaccagaca gcatcactgc atcagtaccg tcgaggatgg cgtttgcaac gtcaccggct
     3361 tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca gcatctgggt cgcagtgata
     3421 acgactttac gtgcacggat acatttttcg atcatcatct tctgggcgaa gataacttct
     3481 tctaccggga tttctacacc caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg
     3541 aggatttcgt cgaagttgtt gaggccttcc tggttttcga ttttggagat gatgtggatg
     3601 ttttcgccgc cgtgcgcttt caggtgctca cggatttcga taacgtcaga acgcttacgg
     3661 ataaaggaag cagcaacaaa gtctacgcct tgttcgcaac caaagatcag gtcctgtttg
     3721 tctttttcag ccagtgctgg cagagcaatg gaaacgccag gcaggttcac acctttgttt
     3781 tcgcccaggt caccgttgtt cagcacttta cagataactt tgttaccttc aatggcggta
     3841 acttccatac cgatcagacc atcgtcaacc agtacggtgt tgccaactga caggtcagta
     3901 gtgaaacctt catacgttac cgcaaccatt tcgctgttgc cgataacaga tttatcagtg
     3961 gtgaaagtaa aggtctgacc agctttcaga gaaacgtcgt taccgccttc cagtttcatg
     4021 gtgcggattt ccggaccttt ggtatcaagc aggatagcgg cggttttacc agttttgctc
     4081 atcacgttgc gcagattctg aatgcgctga ccgtgttctg catagtcacc atgagagaag
     4141 ttcagacgca taacgttcat gccagcgtcc agcattttag ctaacatctc ttcagattcg
     4201 gttttcggtc cgatggtgca aacaattttg gtctttttca tgacagtctt agtctttaag
     4261 ttgagaagga tgggagaaac ttgctttctg ggcgcactgc cggaaagcga atccggtatt
     4321 acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat
     4381 gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg agtcgttgag ttctacagtc
     4441 caaagtgctg aaaccattca agagtcaatt ggcgcgcatt atacgctgaa attaatccaa
     4501 aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg ttagacaata aattgttatc
     4561 aaagatttaa cgttccaggg aaaaggttac gcaaaaactt cactgtttgc gcaacgtctg
     4621 gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc cggtgtataa atatatcatc
     4681 gctcaattgc tacagttaaa ataatttatc gcgttatttg tctgattttg tttgattttt
     4741 gtttaattgg ttagattgat atatgctgat agttggcatg ataaataggt ataaagaatg
     4801 ggaaatcgga caaaagagga tgaactgtac cgagaaatgt gcagagttgt cggtaaagtt
     4861 gtgctggaaa tgcgcgatct ggggcaggaa ccaaagcata ttgttatcgc aggcgtatta
     4921 cgtacagcat tagccaataa acgcattcag cgcagtgagc tggaaaagca ggcgatggaa
     4981 accgtcatta acgcactggt aaaatgacca ttcaggatga ggcggtaagc ttcatcctga
     5041 tattcttgct tcagttgcgg tccagttaat cattctaaat aacaactgct gaaattatac
     5101 gaattgcggt atgtatttta atttatagcg ataatgttat ttttattttc tgcaatgaac
     5161 gtcacaaata attgtcgctt aagttaaatt tatttcaaat gataataaat atcagttcac
     5221 ttataatttc ttgctttgta attttaattg ataacgatca atgttaaggg ctcagaataa
     5281 tcacattatt ttttctccta accataaagg attagtttat cggcgttaaa tcttttctca
     5341 tacactatcg ggttagggaa aatatctggt gttggggaca gagtcagcag cgtattagtt
     5401 gaatgcttaa tatcaggctg ctgaattgtc agcactgtgt ttgcgaagag gatgcgaaat
     5461 aaatgaaccc ggttgatcgt ccactattag atattggctt aacgcgatta gaattcttgc
     5521 gtatatcagg aaaaggcctt gctggtctaa caattgcccc tgcgttactg tcacttttgg
     5581 gttgcaaaca ggaagatatt gatagcggca cagtagggtt gataaataca cccaaagggg
     5641 tgctggtgac ccagcgagcg cgatgcacgg gttgccaccg ctgtgaaatc tcttgtacca
     5701 acttcaacga tggctcagta gggacattct tctcccgtat caaaatccat cgtaattatt
     5761 tctttggcga caacggggtt ggctctggcg gcggcctgta tggcgatctc aactataccg
     5821 cggacacctg ccgtcaatgc aaagagccgc aatgcatgaa cgtctgcccg attggtgcga
     5881 ttacctggca gcagaaagaa ggctgtatta ccgtcgatca taaacgctgt attggctgta
     5941 gcgcctgtac tacggcatgc ccgtggatga tggccaccgt aaataccgaa agtaaaaaat
     6001 cctcgaaatg tgtgttatgc ggtgaatgcg caaacgcctg cccaacaggg gcgttaaaaa
     6061 ttatcgagtg gaaagatatt actgtgtgaa tcttgcaaag gaaaaaatca tggctaacgg
     6121 ttggacaggt aatatattaa gagtcaatct cacgacagga aatattaccc tcgaagattc
     6181 cagtaagttt aaaagttttg tcggtggcat gggcttcggc tacaaaatta tgtatgacga
     6241 agtaccgcca ggcacgaaac ctttcgatga agcgaataaa ttagtctttg ctaccggccc
     6301 attaactgga tctggtgccc cctgtagttc tcgcgtaaat atcacctcac tttctacttt
     6361 taccaaagga aatttagttg tcgatgccca tatgggtggc ttttttgcag cgcaaatgaa
     6421 attcgctgga tacgacgtca ttattatcga agggaaggcg aaatcaccgg tatggctgaa
     6481 gattaaagat gacaaagtta gcctggaaaa agccgatttc ttatggggaa aagggacgcg
     6541 cgcaacgacg gaagaaattt gtcgattgac cagtccggaa acctgtgtgg cggctattgg
     6601 tcaggctggg gaaaaccttg ttcctctctc tggcatgttg aatagccgta accacagcgg
     6661 cggtgcggga actggcgcaa ttatgggttc gaaaaacctg aaagcgattg cggttgaagg
     6721 gacgaaaggg gtcaacattg ccgatcgtca ggagatgaag cgtctcaacg attacatgat
     6781 gactgaactt attggtgcga ataacaacca tgtcgtgcca agtacgccgc aatcgtgggc
     6841 agagtattca gatcccaagt cacgttggac agcacgtaaa gggctgtttt ggggcgcggc
     6901 tgaaggtggt ccgattgaaa cgggggaaat tccgccaggc aatcagaaca cggtcggctt
     6961 tcgtacctat aaatccgttt ttgacttagg tccggcggca gagaaataca cagtaaaaat
     7021 gagtggctgc cactcttgcc cgatccgttg tatgacccaa atgaatattc ctcgggtgaa
     7081 agagtttggc gtgcccagca caggtggtaa cacttgtgta gccaactttg tccataccac
     7141 catcttcccg aacgggccga aagattttga agataaagac gatggtcgtg tgattggtaa
     7201 cctggtgggt ctgaatttgt tcgatgacta cggcctatgg tgtaactacg ggcagttgca
     7261 tcgcgacttt acctattgtt acagcaaagg ggtgttcaag cgtgttctgc ctgctgaaga
     7321 gtatgcggaa attcgctggg atcaactgga agcgggtgac gttaacttca ttaaagattt
     7381 ttactaccgt ctggcgcatc gtgtgggtga gctgagtcac ctggctgatg gttcatatgc
     7441 catcgcagaa cgctggaatt tgggtgaaga gtactggggc tacgcgaaaa ataaactctg
     7501 gtcgccgttt ggctatccgg ttcaccatgc caatgaagcg tcagcgcagg tcggttccat
     7561 tgttaactgt atgttcaacc gtgactgcat gacgcatacc catatcaatt ttattggttc
     7621 gggtttgcca ttgaaactgc agcgtgaagt ggcgaaagag ctttttggtt ctgaagatgc
     7681 ctacgatgaa accaaaaact acacgccaat caatgacgca aaaattaaat atgcaaagtg
     7741 gtcgctgttg cgggtctgtt tgcataacgc cgtcaccctg tgcaactggg tctggccaat
     7801 gaccgtttcg ccgctgaaaa gccgtaatta ccggggcgat ctggcgcttg aagccaaatt
     7861 cttcaaagcg atcaccggcg aagagatgac tcaggaaaaa ttagatttag ctgcagagcg
     7921 tatctttacg ttgcatcgtg cctacacggt aaaactgatg caaaccaaag atatgcgtaa
     7981 tgaacacgat cttatctgtt cctgggtatt cgacaaggat ccgcagatcc cggtctttac
     8041 tgaaggtact gacaaaatgg atcgtgacga tatgcatgct tcgctcacga tgttctacaa
     8101 agaaatgggc tgggacccac agcttggttg tccgacccgc gaaacattgc agcgtctggg
     8161 gctggaagat attgccgccg atctggcagc acacaatcta ctgcctgtgt aaggaatggg
     8221 tgagatgaac catcgggatg aattgccgtt agcgaaagtc agcgaagttg acgaggcgaa
     8281 gcggcaatgg ttacagggga tgcgtcaccc cgttgatacg gtaaccgaac cagaaccggc
     8341 tgaaatcctg gcagagttca ttcgccagca ttctgcggct gggcagctgg tggcgcgcgc
     8401 cgtgtttctc tcgccaccct attcggttgc agaagaggag ctgtcagttt tgctggaaag
     8461 cattaagcag aacggagact acgcggatat cgcttgtatg acgggaagtc aggatgatta
     8521 ctattactca acgcaggcca tgagtgaaaa ctacgcggcg atgtctttgc aagttgtgga
     8581 gcaggacatt tgtcgcgcca ttgcacacgc cgttcgtttt gagtgccaga catatcctcg
     8641 tccttacaaa gtcgccatgc tgatgcaagc gccatattac ttccaggaag cgcagattga
     8701 agccgcgatc gccgcgatgg atgttgcacc tgaatatgcc gatatacggc aggtggagtc
     8761 atcgacggcg gtgttgtact tgtttagcga gcgatttatg acctatggca aggcttatgg
     8821 cttatgcgaa tggttcgagg tagagcagtt tcagaacccc tgaaatttca gggagatgtc
     8881 atggataggg tggacagttg cagccactgc attaggggat aatcagatgt ccttcactcg
     8941 acgcaaattt gttctgggga tgggaacagt aatctttttt actggttctg cgtcttctct
     9001 gttagcgaac acgaggcaag aaaaggaagt tcggtatgcc atgattcatg acgagtcacg
     9061 gtgcaatggc tgtaatattt gcgcccgcgc atgccgtaaa actaaccacg tccccgctca
     9121 gggaagccgt ttatcgatag cacacattcc agtcaccgat aacgataacg aaacgcagta
     9181 tcactttttt cgccagtcat gtcagcactg cgaagatgct ccgtgcatcg acgtatgccc
     9241 gacaggggcg tcgtggcgtg atgagcaggg gatcgtgcgg gtagaaaaat cgcagtgcat
     9301 cggctgcagt tattgcattg gcgcttgtcc gtaccaggtt cgctacctta accctgtcac
     9361 taaagtggca gataaatgtg atttctgcgc tgagtcccga ctggcaaaag gctttccgcc
     9421 catttgcgtc agcgcctgcc cggaacacgc attgatcttt ggtcgcgaag atagcccaga
     9481 aattcaggcg tggttgcagc aaaataaata ctatcaatat cagctacctg gtgccgggaa
     9541 accgcatctg tatcgccggt tcggtcaaca tttgattaaa aaggaaaatg tatgaacccg
     9601 tcgcaacatg ctgaacagtt tcagagccag ttagcgaact atgtgccaca gttcactccc
     9661 gaattttggc cggtgtggtt gatcattgcc ggagtattgc tggttgggat gtggttggtg
     9721 ctggggctgc atgccttgct tcgtgctcgt ggcgtgaaga aatcagtcac cgattatggt
     9781 gagaagattt atctttactg caaagcggtc agattatggc actggtcgaa tgcgttactc
     9841 tttgtattgt tgctggcaag tgggctgata aatcactttg cgctggtggg cgcaactgcg
     9901 gttaaaagtc tggttgcggt gcatgaagtt tgcggatttt tgttactcgc atgctggctc
     9961 ggctttgtgc tgatcaatgc cgttgggggt aatggtcacc actatcgcat tcgtcgtcag
    10021 gggtggctgg agcgagcggc aaaacaaacg cgattttatt tgtttggcat tatgcagggg
    10081 gaagaacatc ctttcccggc aacaacccag tctaaattta atcccttaca gcaggtcgcc
    10141 tatgttggtg ttatgtatgg attgctgccg ttgttactat tgacggggct gctgtgtctc
    10201 tatccgcaag ccgtgggaga tgtgtttcct ggcgtaagat actggttatt gcaggcgcat
    10261 tttgctctgg catttataag cctctttttt atcttcggtc atctttatct ttgcaccacg
    10321 gggcgtacgc cacacgaaac ctttaaaagc atggtcgatg gctatcaccg gcactaacga
    10381 atgatcatta cacgagcaga cctgcgagaa tggcgtatcg gagcggtgat gtatcgctgg
    10441 ttcctgcgcc atttcccacg cggcggtagt tatgccgata ttcatcatgc actgattgag
    10501 gaaggatata cggactgggc ggagagtctg gttgagtatg cgtggaaaaa atggctggcg
    10561 gatgaaaact tcgcgcatca ggaagtttcc tctatgcaaa aactggccac agaccccgga
    10621 gagataccat tttgcagtca gtttgcccgc agcgacgatc atgctcgcat cggctgttgt
    10681 gaggataacg cccgaatcgc aaccgctggg tatgccgcgc aaattgccag tatgggttat
    10741 agcgtgcgga ttggaagtgt cggctttaac agtcacattg gcagctccgg tgaacgcgca
    10801 cgggttgctg taaccggtaa ttcttcgcgg ataagcagcg ctggagatag tagtcggatt
    10861 gcgaataccg gaatgcgagt acgcgtttgt acattgggtg aacggtgtca tgtcgccagt
    10921 aatggtgacc tggtgcaaat cgccagcttt ggcgcaaacg ccagaattgc caacagcggc
    10981 gacaatgtac acatcattgc cagcggtgaa aattcaacgg tagtgagcac gggcgtggtc
    11041 gactcaatta ttctcgggcc agggggaagc gccgcactgg catatcatga cggcgaaaga
    11101 gttcgcttcg ccgtcgccat tgaaggtgaa aacaatattc gaaccggagt ccgctatcgt
    11161 cttaatgaac agcatcagtt tgttgagtgc tgatagcttt acctttaatc aaacgaaaga
    11221 cgccgacgag cacgggatgc aggctttacg accgcccgag ccatcgcctc accaatttct
    11281 gcacatgccg taagtccctg aacgaaaggc tggtcgtgca tcaaccaggg caacgcgccg
    11341 aacgctacgc gtccacgaat atcttcgggt tgctgtaacg tataatcttc gccaacatca
    11401 gggatttcat cccctgtttt ctgtaattgc tcgcgtagcc cagggaaagg aatatctttc
    11461 actttaagcg gacgctgtcc gcgggcatca ataaaaacgt caaacgagta gctgttgtct
    11521 tccgttttca ggacggtgcg cgactcatta atttccattt tgtagtcttc accgagggcg
    11581 agaatatgaa tgattcccgc ttcacgtaag gcaagtaggc gacgaataga ctctgaaggg
    11641 attgccgcat aattatcgat gaaaaccctg gcaaggcctt tactgaaccg tttatggtcg
    11701 tgttcattca gatgaggaac aatttcctgt acggcttcat gcaggcgcag aatgacataa
    11761 cgccagggaa cagtatgttt ttctcgttta ttgcgttcga cttcctggag atttttttct
    11821 gcccagtcaa atgggtcgcg ttgtttgcgc tcggcaaacc aggcttgagc aaaggaatcg
    11881 acattcaggc tctctaaggc tatgcgttgg ctccagtctg gatcagcaaa cttgatttcc
    11941 tctactatca atctaaatac ccgatccaaa aggccctctt cacctttttg aatctcagca
    12001 tttaatgcct gatcggtgac gatgtgtaag ggctcgtagg gaatagggca atagaaatcg
    12061 gcttcgggta aaatacccgt gcgcgacatc aacgtaatat ttagcttttc acttgcgtta
    12121 tcgcggttaa aaacgacgtg ttgtttatca tcttcaatga acgaaccatg ctgaatagcc
    12181 actgccattg ccgcatccag tccgctcaag gatgttccca taatacccac gttacacgca
    12241 tcgacctttg cttccatcag gcctgaccac ggactgggaa aatacgttct ggttgcttct
    12301 tcttcatcag gccagacgtg acccgtggca atcaccgcta aatcaaacgt ctcgctgggt
    12361 aaatcctgat ttgtagcgag catgacgcca gcatttgtaa tttgcagatc ggtaacctgg
    12421 catgattcat aaacagccac tgcaaatttt tgctgtcgtg cctggtctac taatcgtaaa
    12481 aattgatcgc ggaaatattc gcccagcaga attcgcggta aaaactgacg atcgtgcaag
    12541 gtttcttttt taacgccata acgctggaga tggctggctt cttgcttttg tagccattcg
    12601 agatacgtac aattaatcgg cggtatttca atactggcaa tatttgccag catcattttt
    12661 gagttttcct catcactgta tggcatcccg acaccggcct cgtcagcctg ctcgaagata
    12721 gaaatagaaa gtggagtttg ttgctgtaga agcgagaata aggtgtagat ccccgtaggc
    12781 ccggcaccca caatagcaat ttttttcatt gttacttacc tgtacgcatt ctttcgaatt
    12841 tagtgttatc agataagttt ggttgagatt tttagaattt gagaaatttc agacagggat
    12901 gaaaagggcg ctaaccgccc ttgattaaat tctgtcaggc gagttttgcc tgattgattt
    12961 gactcagtgg ctcattgaca tcgaaaactt tggcgacaac ttcctcaacg ccgagatttt
    13021 taagtcgggc agtatgtttt tccagatagg caagggcgct tttttcatca gtaaaaagat
    13081 agatcccacc ggcttcgtgg ttcttttcac tttctgtcca taccttccac agaaaaccag
    13141 gttcctgatt aatcgactca gcaagcggtt taagctgctc agccattgcg tcgccaaatg
    13201 ggccattaaa agcaaaatga agttgtaaca aagttgccat agcgattctc cttctgataa
    13261 cgcatacgaa gacagtgtaa atcttttaac gtattggata cagagaagaa acagagggaa
    13321 aaaggagaag accagattaa gaaaatctgg tgcgtccgag tggactcgaa ccaccgaccc
    13381 ccaccatgtc aaggtggtgc tctaaccaac tgagctacgg acgcaggatg gtgcgttcaa
    13441 ttggactcga accaacgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctatg
    13501 aacgcaacgt tgtaggtgac aacggggacg aatattagcg gcagagtggg aaggtggcaa
    13561 gaggcaaaac gtaattttct gcgctatttc gaccgtttgc agagctttta agcaaattgg
    13621 ctatattttg ttgatttgca agggcgattt ttattcagga tcgcatttac atctgataca
    13681 acccaataat tcgtgtatca gagtttaaac agagttgttt gccaccatct caaatctgtc
    13741 gtggataaat cctccgtagc ctttacactt aggtagcaat aaaggcacac agatgcagca
    13801 aaggaataaa tcatgggatc tgatgcgaaa aacttgatga gcgacgggaa tgtgcaaatt
    13861 gttaagaccg gcgaggtcat tggcgcgacg caacttactg aaggcgagtt gattgttgaa
    13921 gctggcggaa gagccgaaaa taccgtggtc acgggggctg gctggttgaa agtggcaacc
    13981 ggtgggatcg ccaaatgcac acagtacggt aacaatggca cgctatcggt cagcgacggt
    14041 gccattgcca cagatattgt tcagtccgag ggaggcgcaa ttagtctctc tacgctcgct
    14101 acggttaatg gccgccatcc cgaaggtgaa ttcagcgttg atcagggtta tgcctgcggt
    14161 ttgttgctgg aaaatggcgg taacctgcgt gtactggaag gacatcgcgc ggaaaaaatc
    14221 attctcgatc aagagggcgg cctgttggtt aatgggacaa cctcagcggt cgtggtagat
    14281 gaaggtggtg aattgttggt gtatccaggt ggggaagcca gcaattgtga gattaatcag
    14341 ggcggcgttt ttatgctggc cgggaaagcc agtgatacgt tgcttgctgg tggcaccatg
    14401 aataatctcg gtggtgaaga ctctgacact attgttgaga atggatccat ttatcgtctg
    14461 gggacggatg gccttcagct ctacagttcc ggtaagacgc aaaacctgtc cgtgaatgtg
    14521 ggtggtcggg ctgaagtgca tgccggtacg ctggaaaatg cggtaataca aggtggaaca
    14581 gtgatcctgt tgtcacccac cagcgcggac gaaaattttg tcgtagagga agatcgtgca
    14641 ccggttgaac tgaccgggag tgttgcatta ctggacggcg cttcaatgat tattggctat
    14701 ggcgcagatc tgcaacaatc aacgattact gtacagcagg gcggtgtgtt gattctcgac
    14761 ggcagtacgg taaaaggtga cggtgtcact tttattgttg gtaacatcaa tctgaatggc
    14821 ggaaaactgt ggctgatcac tggtgcggca acgcatgtgc aactgaaagt gaaacgcctg
    14881 cgcggagagg gagcgatttg cctgcaaacc agtgcgaaag aaatctcacc tgacttcatc
    14941 aatgtgaaag gggaagttac cggggatata cacgttgaga taacagatgc cagtcggcaa
    15001 actctgtgca acgctctgaa attacagcca gacgaagacg ggattggcgc aacgctccag
    15061 cctgcgtaaa aacacaggcg caacccgtga aggttgcgcc ttgtctttat tagcgggatg
    15121 ctcgttgcag aatgataact gacggcagac gttgcaggaa ccgcatacgc agcatcatca
    15181 taatggctgc cgacgtcagg ccaataataa agcctatcca gaagcctgct ggccccatag
    15241 gttcaacgac cagatcggtc agtgccagaa tatagccgct tggcaagccc agcacccagt
    15301 aagccgtaaa ggtgatatag aaaatggaac gcgtatcttt ataaccacgc aaaatcccac
    15361 tgccaatcac ctggattgag tcagaaatct gatataccgc cgccagcaac atcaaatgcg
    15421 cagccagcgt tacaacctcg ggattgtcgt tgtacaacag ggcgatttgc tcccgcagtg
    15481 aaaccgtgaa tatggccgtc agggttgcca tacagacacc caccataagc ccggtccgcg
    15541 cagcggtttg cgcatccagc gttgagccct gacccagacg ataacctacg cggatagtta
    15601 ccgctgccgc cagcgacatt ggaagcacaa acatcagtga actaaagttc agggcaatct
    15661 ggtgtcctgc gacatcaaca ataccgagcg gagacactaa cagagccacg acggcaaaca
    15721 gtgtcacttc aaagaatagt gccagcgcaa tcggcaaacc gagttgaatc agtcgtttca
    15781 tcaccgcagg atcgggtttc gcggtgcctt tttcgttacg aatatcgcgc atggagcggg
    15841 cgcgtttaat gtaagaaacc atggcaagga acatgaccca atacaccgcc gcagtagcca
    15901 cgccacaacc aacgccaccg agctcaggca taccgaaatg accataaata aagatatagt
    15961 tcaccggaat gttcaccagc aggccgataa agcccattac catacccggc ttggtttttg
    16021 ccagaccttc acattggtta cgggcaacct ggaagaacag atagcccggc gcgccccaca
    16081 acaacgcacg cagataaccc acggctttgt ccgccagagc cggatcgatg ttttccatgg
    16141 agcggataat gtaacctgca ttccacagca caagcataat gagaacggaa acaaaacctg
    16201 ccagccagaa accttgtcgc acctgatgcg caatgcgctc acgtcgaccg gaaccattta
    16261 attgcgcgat aaccggcgtt aatgccagca gcagtccgtg accaaagagg atcgccggaa
    16321 gccagataga agtaccgata gcgaccgccg ccatgtcggt ggcactatag c
//
LOCUS       DAFATA010000101        15922 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFATA010000101 DAFATA010000000
VERSION     DAFATA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.102"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..782)
                     /gene="ydiB"
                     /locus_tag="LGJ20_003808"
     CDS             complement(<1..782)
                     /gene="ydiB"
                     /locus_tag="LGJ20_003808"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBK2387819.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLL"
     gene            complement(794..2059)
                     /locus_tag="LGJ20_003809"
     CDS             complement(794..2059)
                     /locus_tag="LGJ20_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387820.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSTLATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(2286..2762)
                     /locus_tag="LGJ20_003810"
                     /pseudo
     CDS             complement(2286..2762)
                     /locus_tag="LGJ20_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2769..3977)
                     /locus_tag="LGJ20_003811"
     CDS             complement(2769..3977)
                     /locus_tag="LGJ20_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBK2387821.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            complement(4086..4838)
                     /locus_tag="LGJ20_003812"
                     /pseudo
     CDS             complement(4086..4838)
                     /locus_tag="LGJ20_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4938..5294)
                     /locus_tag="LGJ20_003813"
     CDS             complement(4938..5294)
                     /locus_tag="LGJ20_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBK2387822.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5430..5537)
                     /gene="rprA"
                     /locus_tag="LGJ20_003814"
     ncRNA           complement(5430..5537)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="LGJ20_003814"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5723..6835)
                     /gene="ydiK"
                     /locus_tag="LGJ20_003815"
     CDS             complement(5723..6835)
                     /gene="ydiK"
                     /locus_tag="LGJ20_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBK2387823.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            7224..10280
                     /locus_tag="LGJ20_003816"
     CDS             7224..10280
                     /locus_tag="LGJ20_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK2387824.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELYAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEEKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            10277..10687
                     /gene="menI"
                     /locus_tag="LGJ20_003817"
     CDS             10277..10687
                     /gene="menI"
                     /locus_tag="LGJ20_003817"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBK2387825.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10787..10975
                     /locus_tag="LGJ20_003818"
     CDS             10787..10975
                     /locus_tag="LGJ20_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBK2387826.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11524..11892
                     /gene="sufA"
                     /locus_tag="LGJ20_003819"
     CDS             11524..11892
                     /gene="sufA"
                     /locus_tag="LGJ20_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBK2387827.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11901..13388
                     /gene="sufB"
                     /locus_tag="LGJ20_003820"
     CDS             11901..13388
                     /gene="sufB"
                     /locus_tag="LGJ20_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK2387828.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            13398..14144
                     /gene="sufC"
                     /locus_tag="LGJ20_003821"
     CDS             13398..14144
                     /gene="sufC"
                     /locus_tag="LGJ20_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK2387829.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            14119..15390
                     /gene="sufD"
                     /locus_tag="LGJ20_003822"
     CDS             14119..15390
                     /gene="sufD"
                     /locus_tag="LGJ20_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK2387830.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            15387..>15922
                     /locus_tag="LGJ20_003823"
     CDS             15387..>15922
                     /locus_tag="LGJ20_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK2387831.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGT"
BASE COUNT         4221 a         3786 c         4067 g         3848 t
ORIGIN      
        1 cacaacaaca tgccgtatcc atcaatcgtt ttgcaaccag cttgttgcgc ctgctgcaat
       61 aacttcgtca tatgcgggtt atacacgcat tcagtgacca gcagtcccgg atgtaacaga
      121 ctgatatcat taactaatga ttcattctca aggggtttca tacccacttt tgtgccattg
      181 gttaaaatgt cggcggaagc cagggcttca gcaaaggctt gctgatcggc gagatcggta
      241 accgtgacga cacaatcggt gttttcgtta acccgctgcg cgaaggcgag ggctttatcg
      301 aagaactcat cccgacggtt aaagagttta atttctttta aaccttcaat tgccccctgc
      361 gcgccaatcg ccgttgaggc acctccggcc cctaacagca ccattgtttt gcctttgata
      421 tcaaaaccgc tctctttaat ggctcgaata tggcccgtgc cgtcggtgtt atagccgcgc
      481 aggtaaccat catcattaac gatggtgttg atggcaccca ccagtttggc tgctggcgtt
      541 aattcatcaa catattcaca cgccagttgt ttgttcggca ttgatatacc agttccgcgc
      601 attttgaggg cttttaatcc ttcaattgct ccgggaaaac tatcgttatc cacttcaaag
      661 gccatatagg taaatggcaa tcccgctttt tctaaggctt tattctgcat ttcaggcgat
      721 aaactgtggc ggataggata ggccatcagc ccaatcaatt cgtattttgc ggtaacatcc
      781 ataattaact cctttaacct ctatgcttaa ttgtgtttaa tcgggtgcta aatttgcgct
      841 cgccaaaccg cacatcattc tcaggaataa taaaaacgcg gtaatagcgg ataaaaacaa
      901 taattgcggt aatcagggcc agcagcgcga aagtaaaatc gagaacaatg atatattgca
      961 ggccgatgtt cgacagataa ccggtaatca gcggaataac aaagttagcc agtccaccca
     1021 tcatcatata aatactggtg actttggctt tgctttttgg aaaaaactct gacatgaccg
     1081 aaacaccgag ctgtaaaatg ccgccagctg ctgagaaacc gataacaaag gcgccggcat
     1141 tacacaccag tggagaaggg tacaggtaga taatggctgc tgttattgtt gccagtgtag
     1201 agttaaatac attagcccag atgggccgga ccattttttt cagtagtgcg gcaaaaataa
     1261 agacacagac caacgagccc atactgtaat aagagatggt ttttaatgcc tcagcttctg
     1321 acataccagc aaaagccatc gcatatttgg gcatccacac cacaatcaca taaaaggtcg
     1381 agaatgcggc tacaccgaac agtaccgatg aaacaccttc cagccagact aacggtttgc
     1441 tgttcatttg cggtaattca ttagctacgc tggcgtccac caactggctg gggaatttgc
     1501 ttttcaacag catcagcgtg atcagtacaa ataggatgcc tggaataatc agcccatagc
     1561 cgtaccagat gttattgagc aacatatagc tcaccagcat tgggtagaac atttgtccga
     1621 atgacaccat cgctttaacc agtatgaccg ccgaaccaga ggctttcgga aagcattcca
     1681 tgagcgcggg gtagccaccc gtatccagcg ctgagttagc gatacctacg cacactgcca
     1741 gaccgtaggc gagagttaaa ttcgggcaag cgggaatacc aaagaagaat agcagataca
     1801 ttattactgc cattaatatc accgcccgac gaccaaactt atcggagatc acaccgaaga
     1861 ataaaatact gatcaatcgc cccaaaccga taccggaaat taaataggca atgcccgcgt
     1921 tgtcagtgga aaacttttcc gccagagatg acatgttttg ggcaagcgta ataacactaa
     1981 tgccgtgcag gaagtagctg aagtaaatac aaagaacagc caggataaat ggcgtgctga
     2041 aagccttatt ttgagacatt ttcacagctc cttgctgaaa gataatttcg ttaccgtacg
     2101 tattgttctc cgacggttta cataattact atactgaaga tgattttccc tgggtacatg
     2161 gcataaagtt tatgtttcca ggaatattgc atgtcatagt ttgtttttga tcgcgatttt
     2221 ccttagttag cgcagaaaga gttatttatc cgtaagatct tgcatgacct gaaaagaata
     2281 ttgcattacc cacccggagc gacattttcc tttagcgact gatgccgcgt ttttttgcgg
     2341 ctgcgtaagc cgataaacag tgccagtaaa aaaccgatgg cagcgatggt ggtatcgaac
     2401 cacataatat cggcaatact tctttgggac agatgagccg taatcaacgg aatagtaaag
     2461 gtcgcaatac tgcccgcact gtaatagatc cctgtagctt tacctttagc gtaagggaaa
     2521 cgttcagcca ttaacgtcag gccaatttgc acaacacctc cagcagaggt aaaaccaatg
     2581 acaaaagcaa atattatcac cacataaaat gtgggatgca ggcagacggt aaacagagca
     2641 ataaatgaga taaaggtgta cagcatcagt aatgttgttg ggcgaacggt attacgaatg
     2701 agtggagcgg taataaatac acaaagcagc gaacccacgg tgtagatact gagtactgag
     2761 agatcccctc ataatttccc caaaacgtaa ccatgtgtga atagattttg agtaagcagg
     2821 gttgcagcca cgagtgagtc ttcccttgtt attgtgtagc cagaatgccg caaaacttcc
     2881 atgcctaagc gaactgttga gagtacgttt cgatttctga ctgtgttagc ctggaagtgc
     2941 ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc aacatgttag ttgaagcatc
     3001 agggcgatta gcagcatgat atcaaaacgc tctgagctgc tcgttcggct atggcgtagg
     3061 cctagtccgt aggcaggact tttcaagtct cggaaggttt cttcaatctg cattcgcttc
     3121 gaatagatat taacaagttg tttgggtgtt cgaatttcaa caggtaagtt agttgctaga
     3181 atccatggct cctttgccga cgctgagtag attttaggtg acgggtggtg acaatgagtc
     3241 cgtgtcgagc gctgattttt tcggccttta gagcgagatt tatacaatag aatttggcat
     3301 gagattggat tgcttttagt cagcctctta tagcctaaag tctttgagtg actagatgac
     3361 atatcatgta agttgctgat aggtttccag ttttccgctc ctaggtctgc atattgtact
     3421 tttcctctta ctcgacttaa ccagtaccaa cccagcttct caacggattt ataccatggc
     3481 actttaaagc cagcatcact gacaatgagc ggtgtggtgt tactcggtag aatgctcgca
     3541 aggtcggcta gaaattggtc atgagctttc tttgaacatt gctctgaaag cgggaacgct
     3601 ttctcataaa gagtaacaga acgaccgtgt agtgcgactg aagctcgcaa taccataagt
     3661 cgtttttgct cacgaatatc agaccagtca acaagtacaa tgggcatcgt attgcccgaa
     3721 cagataaagc tagcatgcca acggtataca gcgagtcgct ctttgtggag gtgacgatta
     3781 cctaacaatc ggtcgattcg tttgatgtta tgttttgttc tcgctttggt tggcaggtta
     3841 cggccaagtt cggtaagagt gagagtttta cagtcaagta atgcgtggca agccaacgtt
     3901 aagctgttga gtcgttttaa gtgtaattcg gggcagaatt ggtaaagaga gtcgtgtaaa
     3961 atatcgagtt cgcacatctt gttgtctgat tattgatttt tcgcgaaacc atttgatcat
     4021 atgacaagat gtgtatccac cttaacttaa tgatttttac caaaatcatt aggggattca
     4081 tcagtactga gtagtttaat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtac
     4141 tgtgccagcc actggctaac cagataaaac gttgccattg agatatagcc atataaggta
     4201 taactggcta aatcgataat tgaacagcga tgttccgtag agctggtggt tttctttatg
     4261 acaggtaagc gatgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta
     4321 ataaacataa tgcctgcagc aatcataaag gaccaaccga accacagttc agcccacact
     4381 aacaggctaa tgattagcgg taataaaaat tgtccgctgg aaacaaatgc tttaattaaa
     4441 atattggctg tcccaggtga gcgtggaaaa gcttccatca aactgggata agtgcctgca
     4501 tcgagaaaac tgtttgccat tcctgccaga aagccaaaaa cataagcgat aatgatgtta
     4561 ttggtgtgca ggatgccaaa aaagaaggcc atatagcagc acatcccgag catgataaaa
     4621 gggcggcgac caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca
     4681 atgcccagcg atgagataac tatcgagaca cccgcggcat tagtctgcca aagtgtctcc
     4741 agcgaggcca tattcaggct catcaaaatg acgcccatac catgcaccag gtaattaaaa
     4801 tacaacccaa gtgcggtagg gtaataggga tttttcatag gttctactta gcaaaggtgc
     4861 tttaaaaagt gtagcgatgt tgtgtcagta tccgttcatt attgttgtaa caacgacaat
     4921 tcctttttgt ggagaaatta acaaagccgc tccagatcgt gtgcatataa ttcagcggct
     4981 accgattgat agatcttcca gtcaataaaa ttgccatcag ggtagacttg ttgaaacgcg
     5041 gttaagtcgg ggaaaaaacg catggtgttg ttgccgatgc ggttatttat tttctctatg
     5101 atggcatgaa gatgtttttt tcgttgctga cgcatagcca acagttgctc gactacacga
     5161 tcaggaatgg cgcatttgcc attttcccac tgacgccagc tttcactgtc accggtctgg
     5221 gcaatccagg tcgcgcattc atcaatagtc atggcaaaaa tatggcgaag tgcctggagt
     5281 tcataagcgt tcatcgtgta atggctgcat gaaataaaca ataaaatata aataacatta
     5341 tcagcctgct gacggcttga agagagtcac agtatcttgt gcaacattat tggaaagagt
     5401 gaggggcgag gtagcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg gagatgggca
     5461 aagactacac acagcaattc gttgtttcac tcaggggatt tccatgctta taaatcaata
     5521 tgttgattta taaccgtaag ctaatagtag gcatacggac tttttgcgtc gatcagattc
     5581 gtctcattag tttaaaaact gtaaagaatt tgcgacaggt actaccagct gaatggcggt
     5641 aagcaggaaa atatggggaa gagcaatcaa atatcaatta gcgggggtta acaatcaggc
     5701 ggcagagccg cctgatgaga aattacttat tcggtttctc gatttcgcca agttcttcga
     5761 gaatttcttc cggttggtcc gtcggcggcg ggacttcttc cacccacgcg gcaaacagac
     5821 gccaggaaac ggctaacaga accggaccaa taaacagacc gatcatcccg aaagcaatca
     5881 aaccaccaat aacaccagag aggatcagga tcagcggtaa atcggcaccc atgcgaatta
     5941 acattgggcg gatgacgtta tccagcgtgc caaccacacc gctccacact aacaataccg
     6001 ttccccaggt ggtatcgcca gtccagtaga gccagataat cgccggaatc agtaccggca
     6061 acgggccaag ttggacaagg caggagagga tcattaacac cgttagcaac gttgcataag
     6121 gtacgccgga tacggcgagg ccgataccgc caagcactgc ctgtactaac gccgtcacca
     6181 ccacacccag cgccaccgcg cggatagcct gtgccgccag cagtacggcg gcatctccgc
     6241 gaacgcctgc cagacgggtt gcaaaatggc gaatgccttg tgccacctgt tcaccgcgcc
     6301 agtacagcag ggcactgaag agaagcatca gcgcacaatg caccataaag cgcccgatat
     6361 gcgccgcctg cccaacgaac caggtggtgg tggtgccaat ataagggcgg actttcgcca
     6421 tgatcgccgt cccccccata tccagcaagt tgtgccagcc tgcatacagc ttcgcgccaa
     6481 tcaccggaat ggtattaagc cacgccagat cgggtaacgt catgtcgccg ctggaaatgg
     6541 ctttaattag cgggccgctg ccgtcgacga tactgttaac cagcaaagca atagggatga
     6601 taaacaccat cactaataac agcgtcatca ccagaacggc gagagagcgg cggccaaaca
     6661 tgatcttttg caaacgtaac aataccggcc aggtggcgat aaccaccgta ccggcccatg
     6721 caaagccgag aataaagggt tgaacaatcc acagacatgc cacaatcatg atggctaaaa
     6781 acagcaccga aagcagaatt tgtgcgacat ccctgggctg acgaacattt accataacta
     6841 cttttcacct tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga aactcacctg
     6901 cataatgatg agcaatttca gcggttttta acaggccgat tctgccctta attctgatgg
     6961 gcgtgttaga aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat ttactcgtcg
     7021 tatttcaagt tgcatgtgct gcggctacgt tcgttcaccc cagtcactta ctattgtaag
     7081 ctcctggagt tcactcgctt gccgccttcc tgcaactcga aatccataga gtaaataaac
     7141 atcacctttc agtacgctat tgcagccgcc tgaaagaata gtgtatcgac ctacatcaca
     7201 gaccgcagga aagggtcaat ataatgattc cacagatttc ccaggcaccc ggcgtcgttc
     7261 aactggtgct taattttttg caagagctgg agcaacaagg ttttaccggc gatacggcga
     7321 caagttatgc cgatcgtctg acaatgtcga ccgacaacag tatttaccaa cttctccccg
     7381 atgcggtggt atttccgcgt tcaaccgcag atgtggcgct gatcgcccgt cttgccgcgc
     7441 aggaacgcta ctcatcgctg atctttaccc cccgcggcgg cggcaccggc actaacggtc
     7501 aggcgctcaa ccaggggatt attgttgata tgtcccgcca tatgaaccgc atcatcgaaa
     7561 ttaaccctga agagggctgg gtgcgcgttg aggccggggt gataaaagat caactcaatc
     7621 agtacctgaa accgttcggc tacttttttg cgccggaact ttcgaccagc aaccgggcaa
     7681 cgctcggtgg gatgatcaat accgatgcat ccggtcaggg atcgctggtc tatggcaaaa
     7741 cgtcagatca cgtacttggc gtacgcgcgg tgttgttggg gggcgatatt ctcgatacgc
     7801 aacctttacc cgtcgaattg gcggaaacgc tgggtaaatc caataccaca atcgggcgaa
     7861 tttataacac ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc gacaacttcc
     7921 ccaaacttaa ccgctttctt accggttacg atctgcgtca tgtctttaac gatgagatga
     7981 ccgagttcga cctgacgcgc attctgacgg gttcagaagg gacgctggcc tttattaccg
     8041 aagcgcggct ggatattacg cccttgccta aagtgcgccg tctggtgaac gtcaaatatg
     8101 actcttttga ctccgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg gcgctttcgg
     8161 tagagacggt ggactcaaaa gtgctgaatc tggcgcggga agatattgtc tggcattccg
     8221 tcagcgagtt gattaccgat gtgcctgaca aagagatgct cgggctgaac attgtggagt
     8281 ttgctggtga tgatgaggcg ctgattgatg agcgggtaaa tgcactctgt gcgcggttgg
     8341 atgagctgat cgccagtcat caagcaggtg tcatcggctg gcaggtgtgc cgtgagctgg
     8401 cgggggttga acgtatctat gcaatgcgca aaaaagccgt tggtctgctt ggcaatgcca
     8461 aaggtgccgc taagccaatt ccgtttgctg aggatacctg cgtaccgccg gaacatctgg
     8521 ccgattatat tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc tacggtatgt
     8581 tcggtcacgt cgacgcaggt gtcttgcacg tccgtccggc actggatatg tgcgatcctc
     8641 aacaagagat tttgatgaag caaatttctg atgacgtggt ggcgctgact gcgaaatacg
     8701 gtggtttgtt gtggggcgag cacggcaaag gttttcgcgc tgaatacagc ccggcgtttt
     8761 tcggtgagga actttatgca gaactgcgca aagtgaaagc ggcatttgac ccgcataacc
     8821 gactcaaccc agggaagatt tgcccgccag aaggtctcga tgcgccgatg atgaaagtgg
     8881 acgcggtgaa gcgcggtaca ttcgatcggc agatccccat tgcggtacgc cagcagtggc
     8941 gcggtgcgat ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc cgtagtccga
     9001 tgtgtccgtc gatgaagatc acccagaacc ggattcattc accgaaaggg cgcgcaacgc
     9061 tggtgcgtga atggctgcgt ttgttggcgg atcgcggcgt tgatccactc aaactggaac
     9121 aagaactgcc tgaatccggc gtcagtttgc ggacgttaat tgcccgcacg cgcaatagct
     9181 ggcatgcgaa taaaggcgaa tatgacttct cacacgaagt caaagaggcg atgtcgggct
     9241 gtctggcctg taaagcttgt tcgacccagt gccccatcaa aattgatgtg ccggagtttc
     9301 gctctcgttt cctgcagctc tatcataccc gttatttacg tccgctgcgc gaccacctcg
     9361 tcgctacggt cgagagctac gcgccgctga tggcacgcgc gccgaaaacc tttaacttct
     9421 tcattaacca gccgctggtg cgcaaactct cggaaaaaca tatcggcatg gttgatttgc
     9481 cactgctgtc ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg gcaaacatga
     9541 cgctggaaca gcttgaagct ctcaatgcag agcagaaagc gcgcacggtg ttggtggtgc
     9601 aggacccctt taccagctat tacgatgcgc aagtggtggc ggattttgtc cgtctggttg
     9661 aaaaattagg tttccagccg gtgttactgc cattttcgcc aaatggcaaa gcccagcata
     9721 ttaaaggttt tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat ttcctcaacc
     9781 gtatggcgaa gctggggatg ccaatggtgg gcgtcgatcc ggcgctggta ctttgttatc
     9841 gcgatgaata taaactggcg ctgggcgagg aacgtggcga gtttaacgtc ttactggcga
     9901 atgaatggct ggcaagcgca cttgagtcac agccagtggc tacagtcagc ggtgaatcat
     9961 ggtatttctt tggtcactgt accgaggtta ccgccttgcc gggcgcgcca gcacaatggg
    10021 ccgcgatatt tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt tgctgcggca
    10081 tggcagggac ttacggacat gaagagaaaa accataaaaa ttcgctcggg atctatgagt
    10141 tatcctggca tcaggctatg cagcgactgc cgcgtaaccg ctgtctggcg accggatatt
    10201 cctgccgtag ccaggtcaaa cgggttgaag gcacgggggt acgccatcct gtgcaggctt
    10261 tactggagat tattaaatga tatggaaacg aaaaatcacc ctggaagcac tgaatgctat
    10321 gggtgaagga aacatggtgg ggttgctgga tattcgcttt gaacatattg gtgatgacac
    10381 ccttgaagcg acaatgccag tagactcgcg gacaaagcag cctttcgggt tgctgcatgg
    10441 aggcgcatcc gtggtgctgg ccgaaagtat cggttccgtt gccggttatt tatgtactga
    10501 aggtgagcaa aaagtggttg gcctggaaat caatgctaac cacgtccgct cggcacgaga
    10561 agggcgggtg cgcggcgtat gcaaaccgtt gcatctcggt tcgcgtcacc aggtctggca
    10621 gattgaaatc ttcgatgaga aagggcgttt gtgctgttcg tcacgattga cgaccgccat
    10681 tttgtgacgt tgtcaccaga aaagtgtgac gcatattgct tttggtcatt atctgcacag
    10741 taaacggtgg tatactgtgc aggtcttgtc taaaagcgag gatgctatgt ctactcaact
    10801 tgatcccacc cagctggcca ttgaattttt acgccgtgac cagagcaatc tttcacctgc
    10861 ccaatatctc aaacgtctga aacaactaga acttgaattt gctgacctgc ttactctctc
    10921 ttctgctgaa ttaaaagaag aaatctactt tgcctggcgt ctgggcgtgc attgatcctg
    10981 acttaatgcc gcaagttcgc caaatcgacc tggattaacc tccgtccagg ttcgatgacc
    11041 ttcttaatac tgcttatcta cccgcctgat attgttcaaa aataatacta atcgcagttt
    11101 gtgttaaaac ggcgggttag ctttatgaga ttattcttat cgccccttca agagctaagc
    11161 cactgagagt gccggagata agcgccggat ggggtagaaa cccttaagcc tgtgtcgcac
    11221 agacttaagg gttttcttat ttctgaacgc tgcacttttt tacctatctc ttaaatagtc
    11281 attttcaata caactttttc tttttcatca cagccttaat gctccttaga taataaccat
    11341 tatctaacaa tgagatacct aattcttagc gtgcctgtta acccacacat cagggtctat
    11401 gcttattaaa agcaggcaaa aagcatgttt tacggtaaag cccctgcgtt tgctgggttg
    11461 aactgataat cattatcact aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa
    11521 tcgatggaca tgcattcagg aacctttaac ccacaagatt tcgcctggca aggcttaacg
    11581 ctgacacccg cagcggcgat acacatccgt gagctggtgg caaagcagcc gggtatggtc
    11641 ggcgtgcgct taggcgtgaa gcaaacgggc tgcgcgggct ttggctatgt gctcgacagt
    11701 gttagcgagc cggacaaaga cgatctgctg tttgaacacg acggcgcgaa gctgtttgtc
    11761 ccgctgcaag cgatgccgtt tattgatggc acggaagtcg atttcgttcg tgaaggactt
    11821 aatcagatat tcaaatttca caaccctaaa gcccagaatg aatgtggctg tggcgaaagc
    11881 tttggggtat aggcggtact atgtctcgta atactgaagc aactgacgat gtcaaaacat
    11941 ggaccggcgg cccgctgaat tataaagaag gattcttcac ccagttagcc accgatgagc
    12001 tggcaaaggg gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg
    12061 agtggatgct ggagtttcgt ctaaacgcct atcgcgcatg gctggagatg gaagaaccgc
    12121 actggttgaa agcgcactac gacaagctga attatcagga ttacagctac tactcagcac
    12181 catcgtgcgg taattgtgac gacacttgcg cgtctgaacc tggcgcggtg cagcaaactg
    12241 gcgcgaacgc ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg ggcgttcccg
    12301 tgcgggaagg caaagaggtg gcggtggatg ccattttcga ctctgtttcg gttgccacta
    12361 cttatcgcga aaaactggcg gagcagggaa ttattttctg ttcctttggt gaggcgatcc
    12421 acgatcaccc ggaactggtg cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact
    12481 tctttgccgc gcttaatgcg gcggtagcct ctgacggtac gtttatttat gtgcctaaag
    12541 gcgtgcgctg cccgatggaa ctttccacct attttcgcat taacgcggaa aaaactgggc
    12601 agtttgagcg caccattctg gtggccgacg aagacagcta cgtcagctac attgaaggct
    12661 gttccgctcc ggtgcgtgac agctatcagt tacacgcggc agtggtggaa gtcatcatcc
    12721 ataaaaacgc cgaggtgaaa tattccacgg tacaaaactg gttccctggc gataacaaca
    12781 ccggcggtat tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa
    12841 tgtcatggac gcaatcagaa accgggtcag cgattacgtg gaaatatccc agctgcattt
    12901 tgcgcggcga taactccatt ggtgagtttt actcagtggc gctgaccagc ggtcatcagc
    12961 aagcggatac cggcaccaag atgatccaca tcggtaaaaa caccaaatcg accattatct
    13021 cgaaagggat ctctgccgga catagtcaga acagttatcg cggcttagtg aaaatcatgc
    13081 cgacggcaac caatgcgcgc aatttcactc agtgcgactc aatgctgatt ggcgctaatt
    13141 gtggggcgca taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg
    13201 aggcaacgac atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca
    13261 gcgaagaaga cgccatctcg atgattgtta acggtttctg caaagacgtg ttctcagaac
    13321 tgccgctgga atttgccgtt gaagcacaaa aactcctcgc catcagtctt gaacacagcg
    13381 tcggataagg aataaacatg ttaagtatta aagatttaca cgtcagcgtg gaagataaag
    13441 ctatcctgcg tggattaagc ctcgacgttc gtcccggcga agttcacgcc attatggggc
    13501 caaacggttc gggcaaaagt accttatcgg caacgcttgc cgggcgagaa gattatgaag
    13561 tgacgggcgg cacggttgag ttcaaaggca aagatttgct tgcgctgtcg ccggaagatc
    13621 gtgcaggcga aggcatcttt atggccttcc agtatccggt ggagattccc ggcgtcagta
    13681 accagttttt cctgcaaacg gcacttaatg cggtgcgcag ctatcgcggc caggaaacgc
    13741 tcgaccgctt tgattttcag gatttgatgg aagagaaaat cgctctcctg aagatgccgg
    13801 aagatttatt aacccgttcg gtaaacgttg gtttttccgg cggcgagaaa aagcgcaacg
    13861 atattttgca aatggcggtg ctggaaccgg agttatgcat tcttgatgag tcggactccg
    13921 ggctggatat tgacgcatta aaagtggtcg ccgatggcgt gaactcgctg cgtgatggca
    13981 agcgctcatt catcattgtt acgcactacc aacgcattct cgactacatc aagcctgatt
    14041 acgttcatgt gctatatcag ggacgaattg tgaaatccgg cgatttcacg ttggtcaaac
    14101 aactggagga gcagggttat ggctggctta ccgaacagca gtaatgcgct gcaacagtgg
    14161 catcacttgt ttgaagctga agggacaaaa cgctcaccgc aagcacagca gcatttacaa
    14221 caattgctgc gtaccggact gccgacacgt aaacatgaaa actggaaata tacgccgctg
    14281 gaagggctga ccaatagcca gtttgtcagc attgcgggag agatatcccc acagcagcga
    14341 gatgccttag cgttaacgtt agacgctgtg cggctggtat ttgtcgatgg acgttacgtg
    14401 tcagcactga gcgatgcgac tgaaggcagc gggtatgaag tgagcattaa cgacgaccgt
    14461 cagggggtac ccgacgctat tcaggcggaa gtgtttctgc atttgacgga aagcctggca
    14521 caaagcgtga cgcatatcgc cgtgaagcgc ggtcaacggc cggcaaagcc attgctgtta
    14581 atgcatatta cccagggcgt ggcaggtgaa gaggtcaaca ctgcccatta ccgacatcat
    14641 ctggatctgg cggaaggtgc cgaagcaacg gtgatcgaac attttgtcag ccttaatgat
    14701 gctcgtcact tcaccggcgc acggttcact atcaatgtcg cagcgaacgc ccacttgcag
    14761 catatcaagc tggcgtttga aaacccggtc agtcaccact ttgcccataa cgatttgttg
    14821 ctggctgacg atgccaccgc atttagccat agtttcctgc tgggtggcgc agtgttacga
    14881 cacaacacca gtacgcaact caatggcgaa aacagcacgc tgcggatcaa tagcctggcg
    14941 atgccggtga aaaacgaggt gtgtgatacc cgtacctggc tggaacacaa taaaggtttt
    15001 tgtaacagcc gacagttgca caaaactatc gtcagcgaca aaggccgcgc ggtatttaac
    15061 ggtttgatca acgtcgcgca gcacgccatc aaaacggatg gtcagatgac caacaataat
    15121 ctgctgatgg gcaaactggc ggaagtggat acaaaaccgc agctggaaat ctatgcagat
    15181 gatgtgaaat gcagccacgg cgcgacggtg gggcgtattg atgatgaaca gatgttctat
    15241 ctgcgctcgc gcgggatcaa tcagcaggat gcccagcaga tgatcattta cgcctttgct
    15301 gccgaactga cggaagcact gcgtgatgag gggcttaaac agcaggtgct ggcccgaatc
    15361 ggtcaacggc tgccaggagg tgcaagatga ctttttccgt cgacaaagtg cgggccgact
    15421 ttccggtgct ttcgcgtgag gtaaacggtt tgccgctggc ttatctcgac agcgccgcca
    15481 gtgcacagaa accgagccag gtgattgacg ccgaggccga gttttatcgt catggctacg
    15541 cggcggtgca tcgcggtatt cataccttaa gcgctcaggc gaccgagaaa atggagaacg
    15601 tgcgcaagcg ggcatcgctg tttattaatg cccgttcggc ggaagagctg gtgttcgtcc
    15661 gcggcacgac ggaagggatc aatctggtcg ccaatagctg gggcaacagc aatgtgcggg
    15721 cgggcgataa catcatcatc agtcagatgg agcaccacgc taacattgtc ccctggcaga
    15781 tgctttgcgc acgcgttggc gcagagctgc gtgtgatccc tctcaacccc gacggtacgc
    15841 tgcaactgga gacgctgcct acgctgtttg atgagaaaac tcgcctgctg gcaattactc
    15901 atgtctccaa cgtgctggga ac
//
LOCUS       DAFATA010000102        15880 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFATA010000102 DAFATA010000000
VERSION     DAFATA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.103"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..106)
                     /locus_tag="LGJ20_003824"
     CDS             complement(<1..106)
                     /locus_tag="LGJ20_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK2387832.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSES"
     gene            416..946
                     /gene="ppa"
                     /locus_tag="LGJ20_003825"
     CDS             416..946
                     /gene="ppa"
                     /locus_tag="LGJ20_003825"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBK2387833.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(1026..1376)
                     /gene="chpB"
                     /locus_tag="LGJ20_003826"
     CDS             complement(1026..1376)
                     /gene="chpB"
                     /locus_tag="LGJ20_003826"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HBK2387834.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(1370..1621)
                     /gene="chpS"
                     /locus_tag="LGJ20_003827"
     CDS             complement(1370..1621)
                     /gene="chpS"
                     /locus_tag="LGJ20_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HBK2387835.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(1834..2175)
                     /locus_tag="LGJ20_003828"
     CDS             complement(1834..2175)
                     /locus_tag="LGJ20_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBK2387836.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(2178..5957)
                     /gene="tamB"
                     /locus_tag="LGJ20_003829"
     CDS             complement(2178..5957)
                     /gene="tamB"
                     /locus_tag="LGJ20_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBK2387837.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(5954..7687)
                     /gene="tamA"
                     /locus_tag="LGJ20_003830"
     CDS             complement(5954..7687)
                     /gene="tamA"
                     /locus_tag="LGJ20_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBK2387838.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            7893..8531
                     /gene="msrA"
                     /locus_tag="LGJ20_003831"
     CDS             7893..8531
                     /gene="msrA"
                     /locus_tag="LGJ20_003831"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK2387839.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            8854..10197
                     /locus_tag="LGJ20_003832"
     CDS             8854..10197
                     /locus_tag="LGJ20_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK2387840.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLV
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(10259..10465)
                     /locus_tag="LGJ20_003833"
     CDS             complement(10259..10465)
                     /locus_tag="LGJ20_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBK2387841.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            10790..11347
                     /locus_tag="LGJ20_003834"
     CDS             10790..11347
                     /locus_tag="LGJ20_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBK2387842.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(11337..12077)
                     /gene="cysQ"
                     /locus_tag="LGJ20_003835"
     CDS             complement(11337..12077)
                     /gene="cysQ"
                     /locus_tag="LGJ20_003835"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBK2387843.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEARQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            12267..14210
                     /gene="cpdB"
                     /locus_tag="LGJ20_003836"
     CDS             12267..14210
                     /gene="cpdB"
                     /locus_tag="LGJ20_003836"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBK2387844.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(14328..14708)
                     /locus_tag="LGJ20_003837"
     CDS             complement(14328..14708)
                     /locus_tag="LGJ20_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK2387845.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            14797..15657
                     /gene="qorB"
                     /locus_tag="LGJ20_003838"
     CDS             14797..15657
                     /gene="qorB"
                     /locus_tag="LGJ20_003838"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBK2387846.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEVGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEVDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            15756..>15880
                     /locus_tag="LGJ20_003839"
     CDS             15756..>15880
                     /locus_tag="LGJ20_003839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HBK2387847.1"
                     /translation="MPVMISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYL"
BASE COUNT         3810 a         4268 c         3932 g         3870 t
ORIGIN      
        1 cggattccga gccgacctgc gaaaatccta cggttaatgg agcggccaac gccatagacg
       61 acatggctgc cgagactgca gagactacaa gtaagcgttt ccacataggg ttgtcctcgt
      121 cggggtaatt attattggtg aaaagatgtt cgcggaaaaa ctatagacaa ttcgttatgt
      181 aacggattgc gttacatcac acttcataaa agtaaataaa acgttaatca caagtttgta
      241 atcgctttca tctcactatg aaaaatgcgg ctacggttat ggattttcct gctctgtata
      301 ccgtcttaaa actggcgaaa aaggaaaatg aagacgaaaa caagcgaaga cattcggcgc
      361 gagttggcta taatactcgg cacttgtttg ccacatattt ttaaaggaaa cagacatgag
      421 cttactcaac gtccctgcgg gtaaagatct gccggaagac atctacgttg ttattgagat
      481 cccggctaac gcagatccga tcaaatacga aatcgacaaa gagagcggcg cactgttcgt
      541 tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac tacggctata tcaaccacac
      601 tctgtctctg gacggtgacc cggttgacgt actggtcccg actccgtacc cgctgcagcc
      661 gggttctgtg atccgttgcc gtccggttgg cgttctgaaa atgaccgacg aagccggtga
      721 agatgcgaaa ctgattgctg ttccgcacac caagctgagc aaagaatacg atcacattaa
      781 agacgttaac gatctgccag agctgctgaa agcgcagatc gctcacttct tcgagcacta
      841 caaagacctc gaaaaaggca agtgggtgaa agttgaaggt tgggaaaacg cagaagccgc
      901 taaagctgaa atcgttgcct ccttcgagcg cgcaaagaat aaataagttc ttatagcgca
      961 ataaccctga acaccgggct tcggttagta agggtttttt tatgcccgcg ataaataaac
     1021 acaccttatt ccaccaccgc ctgcaagcgt aataacgcct cttccaccac ctcatccgca
     1081 gccagaccaa tacgctttgc cagccgggcg cgtagatcca tcatccgcac ctgattcacc
     1141 agcaccacgc cgtgcacatc gccttcttcg caatgtaaag gaacgctaaa tccggcataa
     1201 cgggcaaaat ttccgccctg cgtaatgggg gccaccagcg tcattcccag ttgattaaag
     1261 gcttgaacgg agagcacaag cgcaggtcga ccagcacctt gctgttcatg gccgcttgct
     1321 ggatcaaagc caaccagcac aatgtctccc cgttcaaatt cacttttctt taccatattt
     1381 cgtcacccgc aggggtggat ttaccccaga catcctgctc gctaagttcc gcggcgttca
     1441 tgtcacactg tgccagcagt tcatcaagcg agtagcgcct ggagatgggt gtcagaatca
     1501 gttggttgtt gctcacctgc gcttccacgc tctgccccgg ctgtaagtta agttctttca
     1561 ttacgatatt gggaatgacc atacctgcac tgttccccca tctttttatg gtaatacgca
     1621 tctgcacctc cttaagttat acaaagtata actcacggtg ctacaggaaa cgttttaccc
     1681 ctgcacattt gtgaacgcca gcagagtttt ttatgcgatt gcagatatgg aaagcggctc
     1741 ggagagaaaa gcaaaagatg gaaaagttac aaaaaaaaat cgcgagatac atcgcataaa
     1801 acaacgccac ccgaaggtgg cgtgctttta ttattactta tccctgtcta accagtcgcc
     1861 gctttcaatt agctttaatc catcgacggg tcgttgataa acgtacatcc atgcactccc
     1921 gtacggcgtc tgaatcaact ggcgcgcgta ttcaccgccc ctggtgcgca aggcatcaag
     1981 ttcggccagc gtggcgttgt caatacgata aacttcaccg tgtactgttc cgttccccgg
     2041 aactgcgcct ggatagtggc ccaggctata caactggtag ttatcgatac tgaaatcgcc
     2101 cagtaactgg gcattggtca tccagtgact gttgccttgt ttgtggcgta aactgccgta
     2161 gacaaatatt cgcattgcta aaactcgaac tgatagagca aatccagtgc ctggtctaca
     2221 ccagacacgg cttccagata tagcttaggc atcaggcgat aacgtaacgt gagtgttgct
     2281 atagagtcaa atatacccac gccatatttc acttgcagac ctggcaatac atagccgctg
     2341 accactacct gggaggagtc gcctactccc tgggtgtcga gcgctaaatt gcttacgcca
     2401 aacgtctcgc cgattttacc cacaatctgg ccactttgcg caacccccag accaatcagc
     2461 atcgaggtca ttgccgcact gtcgctctga tcgctctcca gcccttgtcc acgtagcaaa
     2521 taagacaatg cagcttgttg cgacatcgcc gggtcagaga agatctccgc tttcggttcg
     2581 tccgccagac cagtgacgcg aactccggcg attacgtcgt cttctgtagc atccgggtta
     2641 cgaatagctt caatattaag atacggttga tccggcggac cagagaacag taactcaccc
     2701 ttacgcacaa tcagatcctg accataggca tggaagcgcc cttcagggat gttgatctgc
     2761 ccgttcaggc ccagcccttg tttgtcctgt acgacgttaa gatcgcccgt cagccgcgct
     2821 ttcaggccaa aggcgtcaat gcgcacattg ttgccaacgt ggacaatcag gttactgtta
     2881 atcggaatcg acgccgtttt cggctcttcc ggttgcaggt tatcgttaag catcaccaca
     2941 tcgctggaga cgcctactgc gctttccggc agatcgtgca ccacgatgcg cgcccacggg
     3001 acatccacgc gaccatcgag ggtaaacagg tttggtgtag cctcgaatac aacatctggc
     3061 gatacatcca ttcgtaccat cggcggcacg gtgatccgca ctttactgcc tttcgccgtt
     3121 actcgtgccc gccagttttc aatttggctc cagtcggcat caccgttcag gtagatttca
     3181 ccctgctggg tccgtactgt accggcaagc gtcgagcgca taccgttaaa gttgaccgca
     3241 agctggctcg gctgcatatc aaacggcata aagttgccgt cgatatccac acccgtaacc
     3301 tgaagctgac caaacaactg cgggctttgc acatcaccac ccagacgcaa gttggcactc
     3361 accatccccg ctgctttttc cccacgggta aagatggggt ttatcatcgc aaggttgaag
     3421 ttacggatat tgacgttgcc accaagatta cggcggcctt gcggatcggt cacctgcacc
     3481 tgtccatcaa actggccgtt attggtcagg cggatggtcc agcccaattc ggcacggttg
     3541 ttacgcaatt ccgccgtcag attcagtgtc tgaaacgcca ccggcagcgc cgcatcgttg
     3601 acggtttgcg ttacctgcac gttacgcccc gaaagggtga tactgccctg cggcagcccc
     3661 tctttcgtgg tgtcccaggc aacatccgct ttacccgtga agataccgct ggcctgagtg
     3721 gtttctggca taaacggttt cagcatggcg aggtcgaagc ggttgagatt caccaccgca
     3781 cgcccttcgg cccccgcatc gatagtttgc ggcacgcaca gttccgcatt cgggttaagc
     3841 cagcagtgtg gcccgatgct gattttttgc tccttattgc ggtaatccag cgcaatatcg
     3901 cgggtcagcg accacgggcc gaccggcgtc tggaagcggg tattgctaag agttcccttc
     3961 cagcgttctt ctttgcgatc aaaacttcct gccagattaa gctgcccgga gacaggctcg
     4021 ccctgaatcc gcaactgtag ctcgtgctgc ttttcgctgc ctttggcatt cagggtgacg
     4081 aggttgatat tgacatccgg ctgcgaaatt tgctcaacgc gtacgtcgag tttacccgca
     4141 atctgatcgg tggatttgat atcgccttcc acgcgaacct gcgccacgga aagttcctgc
     4201 cagcgcaggc cgcgcgcggt gatatctgcc agtagttgtg gcgcttccac cgtgccgcgt
     4261 actttcacca gccctttcgc tgtaccgcca agccccggca gcgcgttatc cagccccggc
     4321 gcgttgatgg tggcatcaag attgagatct tttaccccca gctcgccttt cacttcggca
     4381 ctgtttggcc ccagttccag atgcagccct gggatcatcc actgcatata actgttgcct
     4441 ttcagcgtgc cgtcaacatt cactttgttc tgtttgacgt taccggtcag cttcagttct
     4501 ggcacctcca tctgccaggt gccgccgtac aggctaccgc gggttttaat caagccattg
     4561 agtttcgacg gccactctgg gatctctttg gcggtgttaa tgccgttaag cgttagctca
     4621 ccgcgccaac taatggcctg ctgccagtcg agcaacgcct tgagttcagt tttcccttcc
     4681 agcgccgcga cggtgagttt gtcgagattg acctgctgtt cattaccttt ggcatcaagg
     4741 gtaatggtgg ctggcgggat ctccagtccc ttcactgccg tacgcataga gagcgtgtaa
     4801 tcggtcattt tgccggtaag tttcagtttc agatcatccg cctgatactg cttctcacca
     4861 gtgaacggcc agtaaatctg tttgctgttc acttccacgt tgagcggcaa tccggcttcc
     4921 gccagtcgcg tctgggcgcg taaatccata tccaccggac cggaaagatt aacgccaatc
     4981 tccagctgtt cgcgcagcgc gccgcccact tttagcttca ctttttcacc tttcaacggc
     5041 tccacgttca gtgtgctgtt gagggtgata tccaccggcc agttgtctga cagctgcgcc
     5101 gtgccgctgg cgttgacgat cccctggttg gaatcgatat ccagggcgtc cagtttagta
     5161 ttgccgtcaa tgctgctcac tttcagcagc atggtgcgca cggtgatgtc cgtgtcgccc
     5221 gtcacgcgta actgctcgcc tttaaactct tcaatgttca ggttaagcgg caaatgcacg
     5281 tcggtcattt ccggcaatac cgggcgagaa aagagatctt tcagcgtttc gccgagcggc
     5341 ttttcatccg gctgcggatt ttcaatcttc ggttcgacga cctcttcctg cgccacttcc
     5401 gccactttcg gcagagcaat cagcaggcct ttcagcgacg tcggtttcag ggtcagggtt
     5461 ttctcctgcc agttcaggcc ggaggtgaag tccatcaccg ataccgtggt gtcatcaatc
     5521 ttgatgttga cgttatccag cgccacccgt gtcagggtga tgggatacgg cgtggagaga
     5581 tccagcggac cgctatcttc ctcttcttca acctgttcag aaggaggcat ttttttactg
     5641 tcgatgttga cctgaatgtc tttcagcgcc aggtcattaa tacaaacgct actgttccac
     5701 aggcactcaa gcccgaccgc cagatgcaga ttgcccgctt ttaccgccac gcctggctgc
     5761 tcataacgaa cgtcagacaa ggtgagatcg cgccagccgc cggtgacctt gccaatatcc
     5821 agtcctggta cccagcgatc cgccgcttta aataccagat gcaggccact ggtggtgccc
     5881 accagaaacg ccaccgatcc cagcaacagt aagataacga taaccacgcc gaggctgatt
     5941 tttttccata aactcataat tctggcccca gaccgatgta aaactgtaac ccgtgttcgt
     6001 ctttatccgc gaccggtacg gcaaaatcga gtttgattgg cccgaccggc gattcccagc
     6061 gcacgccgac cccggtaccg gtcttaaagt cgctgcggcg aatatcgctt accgcttcgc
     6121 cactatcgac aaacaccgcg ccccaccatt ttccggtcac gttgtactgg tactccagcg
     6181 atccggttat caacttcgag gcccctttca ggtcaccgtt ggcgtatttc ggggcgatag
     6241 atttgtattt gtagccgcga atactgcgat cgcccccggc gaagaaacgc agatccggcg
     6301 gtactttgtc gaaatcaccg gtttcaatcc agcccagcgt gccgcgtgta acaaaacgat
     6361 ggcgatcgta cagtgtgcgg atccagacgt tttgtgcctg gaaaacggag aaatcgacat
     6421 ctgagcccca ggccgtgttg gagtagtcga tagagtagcg ttgcgagtcg ccccaggttg
     6481 gcatcaagcc accacgagaa cgcgtgcggc taatcatcac ccctggataa aacagcatcg
     6541 tggtgttggt aatttcaccc tgagtaaagt ggtcgagact ccagcgcagg ttaatggcac
     6601 gctgccagcc gctggagaga tcccagtagc gagaagccac cagcgtagtg gagtcagact
     6661 cggtatcgtt caggtcagtg cgcttaaaac cgccctgcac caaataatat tgttccagtg
     6721 gattcttcag cagcggcatt ttatagctga agtcgagggt ctgttccggc gcggaaatac
     6781 tggtactggt ggtcagactg tgaccataag agttcatcca cggctttttc cacgtcgctt
     6841 tcacgcgcgg tcccacgtcc gtagagtaac cgaccccggt ttcgatggta ttttcagttc
     6901 gcggcgaaac cacgcccgtc aatggtaata ctttcgtttc gcgcgcttta tcaaattgtg
     6961 gagccactac gaccgagtta aaccagccgg tagcagaaag tcgacggttc agttccgcca
     7021 gatctttcga ttcgtactca tcgccctctt taaacggcac cagattttgc aggtattcat
     7081 cgcggatttg tgatccttca aaggtcacat gcccaaagcg gtaacgttcg ccactgttat
     7141 aatcaatatc ccagaaggct ttatgcaggc cgagcgcaat gcccagctgc gctttggtaa
     7201 attcgctatc gaaataacct ttacgcaacg caatgctggt taaggacttt ttgaaatttt
     7261 cataatcgcc ctggttcagt accgtgccaa tagccgggcg agtatcgagc aatttcaaat
     7321 agtctttatc ggtccgcgcg ccgccgcgca ataccacatc ggtgccgcca attaacaccg
     7381 gcacgcctgg cgtgactttg gcgatcaata cctgccgccc tttctttggc ggtggacgga
     7441 gatcaaattc aatggtcggc tggtaataac ccagcgcttt cagaccttcg cggatggcat
     7501 catcgacgcg tgcgcgaaag cgacggtctg gcgtcacttc atcactttca atcgtagaaa
     7561 gctgcgcacg aacgttcttt tccagctgtc ccgataaccc ctcgacctgt agacggacgt
     7621 tcgcggcgac ggcagatccg cttaagcaga gtaagcttac acagcataac tgtcggatat
     7681 agcgcacatt ttctcctgaa tatccttttt tcctgcccct ggaacgccgt taaacggctt
     7741 aacaaaaatc cagtaatatg gattaaaaag cagactaaac cccaaatatt tcttatgttt
     7801 tactttagac ctattcacgg tggttattgt gtgcaaatac gcctcttgtt acaaccttaa
     7861 ccccaatgac cgattttcgg gagagcgaca ccatgagttt atttgataaa aagcatctgg
     7921 tttcccccgc cgatgccctg cctggacgta acaccccgat gcccgtagcc acgctgcatg
     7981 cggtcaacgg tcactcaatg accaatgtac ctgacggaat ggagattgcc atttttgcga
     8041 tgggttgttt ctggggtgtg gagcgtctgt tctggcagtt acccggcgtt tacagcaccg
     8101 ccgcaggcta taccggcggc tatacgccaa atccgactta tcgggaagtg tgctccggtg
     8161 atacgggtca tgccgaagcg gtacgcattg tttacgatcc ttccgtcatc agctatgagc
     8221 agttgctaca ggtattttgg gagaatcacg atcccgccca gggaatgcgt cagggcaatg
     8281 accacggcac gcagtatcgt tcagcgattt atccgctgac cccagaacag gatgccgcag
     8341 ctcgcgccag tctggaacgt tttcaggcgg cgatgcttgc cgccgatgac gatcgtcgca
     8401 tcaccacgga aatcgctaac gccacaccgt tttattatgc cgaagatgac caccagcaat
     8461 atctgcataa aaacccgtat ggttactgtg gaattggcgg aattggcgtc tgtctgccgc
     8521 cggaagcata gcgttacggg tacaaatgta gattgtcgat aaagtgctat ttgtctatgc
     8581 cggatgcggc gtaaacgcct taccaggcct acaaaatcgt ccaaattcaa tatattgcat
     8641 ggactgcgta ggcctgatcg gcatagcgca tcaggcagtt ttgcatttat cagcagcctg
     8701 aattttaacc ctctggcggc tttacagtac cttacgctat actagccact gaaaatgccg
     8761 gttcactttc ttcgaatcgg ctttcaatgt gtatttcaca caaattaatc aacttcccct
     8821 tccgaggatc tggcctgaaa ggtcggataa gatatgttaa acagtatttt agtcatactc
     8881 tgcttgatcg ctgtaagtgc gttcttctcg atgtccgaga tctcacttgc cgcctcacgc
     8941 aaaatcaaac ttaaactgct ggctgatgaa ggcaatataa atgcccaacg cgttctgaat
     9001 atgcaggaaa atcccggcat gttctttacc gtggtccaaa tcggtctgaa cgcagtggcg
     9061 attctcggcg gtatcgtcgg tgatgcggca ttttctccag cttttcacag cctgttctcc
     9121 cgctatatgt cggcagagct ctctgagcaa ctgagcttta ttctctcttt ctcgttagtg
     9181 actggcatgt ttatcctgtt tgcggattta accccgaaac gcatcggtat gattgcgcca
     9241 gaagctgtcg ctttgcgtat catcaacccg atgcgcttct gcctgtacgt ttgcaccccg
     9301 ctggtgtggt tcttcaacgg cctggcgaac atgatcttcc gtattttcaa actgccaatg
     9361 gtacgtaaag atgacatcac ttctgatgac atctacgcgg tagtggaagc cggtgcgctg
     9421 gcgggcgtgt tacgtaaaca ggaacacgag ctgattgaaa acgtctttga gctggaatcc
     9481 cgtaccgttc cgtcttcaat gacaccgcgt gaaaacgtga tttggtttga tctccacgaa
     9541 gatgagcaaa gtctgaagaa taaggtggcg gaacatccgc actctaagtt cctcgtctgt
     9601 aatgaagata ttgaccacat catcggttat gtcgattcta aagacctgct gaaccgcgtg
     9661 ctggctaacc aaagcctggt actgaacagc ggcgtacaaa ttcgcaacac gctgattgtg
     9721 ccggatacgt taaccctttc agaggcgttg gaaagtttta aaaccgcagg tgaagacttc
     9781 gcggtgatca tgaacgagta cgcgctggtg gtggggatca tcaccctcaa cgacgtgatg
     9841 accacgctga tgggcgatct ggtcggtcag gggctggaag agcagattgt cgcccgtgat
     9901 gagaactcat ggctgattga cggcggcacc ccaattgacg acgtcatgcg cgtgctggat
     9961 attgacgagt tcccgcagtc gggcaactac gaaaccatcg gaggctttat gatgtttatg
    10021 ctgcgtaaga tcccgaaacg caccgattcg gtgaaattcg ccggctacaa atttgaagtg
    10081 gtggatatcg ataactaccg catcgaccag ctgctggtga cccggatcga cagcaaggcc
    10141 accgcccttt cgccaaaact gcctgacgct aaagataaag aagaaagcgt cgcgtaaccc
    10201 ccagaaacat caacggctcc tgaatcagga gccgtcttat tactgcatag cactttggtt
    10261 aagccatctc tgtttgcaga cgcataacct gacggttaac ttcggacatc actgacaaat
    10321 gcagcttatc cttcactttt gggataagaa tcttaccctt atcaaattca aaagcgccaa
    10381 cgtccttgat gtataaccgt ccacggaaca ggatctttac gtacttcgcc acctgaagtg
    10441 ggttgtaccg ttggaaaatt ttcattgtta tctcctgctg agtattacgc ctgtgcggtg
    10501 ccacaatcgg cccaactatt atgaggcgca aattttaatg cctagtgact atagactatc
    10561 cgggcaatgt ttccaccgcg tataactttt tttacctaaa ggttacaatt attcagaatt
    10621 atctttttac cgaagcgcgg tcttcagtat aagcattcat ttttcatatg atttgtgcgc
    10681 ttgaccgcaa actggcatca cacttgcggg aaattcgata aatagcacat atgattaaaa
    10741 ctcagaccca agtggtcgga tcacctgcat atcataagaa ggaaacacca tgaccctacg
    10801 caagattctg gcactcacct gcctgctgtt gccgatgatg gcttccgcac atcagttcga
    10861 aaccggtcag cgagtgccgc cgattggcat caccgatcgt ggcgagttgg tgcttgataa
    10921 agatcagttt agctacaaaa cctggaacag cgcgcagtta gtgggaaaag tgcgagtact
    10981 gcaacatatt gctggtcgca cctctgcaaa agagaaaaac gcgacgctga ttgaagcgat
    11041 taaatcagcg aagttaccgc acgatcgtta ccagaccacc accattgtta acaccgacga
    11101 cgcaattccg ggttccggca tgtttgtgcg cagcagtctg gagagtaata aaaagcttta
    11161 tccctggtcg cagtttattg ttgatagcaa tggcgtcgca cgcggtgcct ggcagctgga
    11221 tgaaaaaagt tccgctgtag tggtgctgga taaagacggt cgcgtgcaat gggccaaaga
    11281 cggggcgctt actcaggaag aggtgcagca agtgatggac ctgctgcata aattaattaa
    11341 taaatagaca ctctgaaccc aggattcagg aacgactcgc gcggagtgta atccagcggt
    11401 ttaccctgcc agtcgtgaac gtgcgctccg gcagccgcag ccacagcatg accagcggcg
    11461 gtatcccaaa tattcgttgg cccgaaacgc gggtacagct gcgcctgtcc ttccgccacc
    11521 aggcagaatt tcagcgaaga gccgatggac gtggtctgat gttcgccaag ctgttgcaga
    11581 tactctttca gctccgcatc cgcatgggaa cggctgatca ccaccagcgg cgggcgcgca
    11641 tcgcggacct gaatctgctt gcgcacgccg cactcttctt tccacgcttt gccttctgcc
    11701 gcgctgtaca ttacgttcat taccggcgca tacaccacac ctaaaatcgg tttgccatgg
    11761 tcaatgagtg caatgttaac ggtgaattcg ccattacgtt taataaactc tttcgtacca
    11821 tccagcgggt ctaccagcca gtaacgctgc cagtgctgac gggcttccca accgggagga
    11881 tcttcttcag aaaggaccgg aatatccggt gtcagcgtac gtaaaccgtc catgataacg
    11941 gtgtgtgcgg caatatccgc tgccgttacc ggagaattgt ccgctttgct gacgacgtcc
    12001 atcggtttcg tcccgtcgta gacctgcata atggcatcgc ctgcattccg tgcaagctgg
    12061 catacttgat ctaacatttc tccacctcgt ctctgtgagc ggtgttaact tattgtttta
    12121 cttataccct atcgttaatg aatgcgccaa ctgtgatagt gtcatcattt tcaaagcgta
    12181 aaattgtggc attcttcact gttctataag taagacgttt attctccctt ttctttcgta
    12241 ttcccgatga taaaaggatg tccctgatga ttaagtttag cgcaacgctc ctggccacgc
    12301 tgattgccgc cagtgtgaat gcagcgacgg tcgatctgcg tatcatggaa accactgatc
    12361 tgcatagcaa catgatggat ttcgattatt acaaagacac cgccacggaa aaattcggac
    12421 tggtacgtac ggcaagcctg attaacgatg cccgcaatga agttaaaaac agcgtactgg
    12481 tcgataacgg cgatttgatt caggggagtc cgctggccga ttacatgtcg gcgaaaggat
    12541 taaaagcagg tgatattcac ccggtctata aggcattaaa tacgctggac tataccgtcg
    12601 gaacgcttgg caaccacgag tttaactacg gtctggatta cctgaaaaat gcgctggcag
    12661 gagcgaaatt cccttatgta aatgccaacg tcattgacgc cagaaccaaa cagccaatgt
    12721 ttacaccgta tttaattaaa gacaccgaag tggtcgataa agacggaaaa aaacagacgc
    12781 tgaagattgg ctatattggc gtcgtgccgc cacaaatcat gggctgggat aaagctaatt
    12841 tatccgggaa agtgacggtg aatgatatta ccgaaaccgt gcgcaaatac gtgcctgaaa
    12901 tgcgcgagaa aggtgccgat gttgttgtcg ttctggcaca ttccgggctg tctgccgatc
    12961 cgtataaagt gatggcggaa aactcagttt attacctcag tgaaattccg ggcgttaacg
    13021 ccattatgtt tggccatgct cacgccgttt tcccgggtaa agattttgct gatatcgaag
    13081 gggctgatat caccaaaggc acgctgaatg gtgttccggc ggtaatgccg ggcatgtggg
    13141 gcgatcatct tggggtggtc gacttacaac tcagtaatga cagcggtaaa tggcaggtga
    13201 cgcaggcgaa agcggaagca cggccgattt acgacatcgc caataaaaaa tccctcgcgg
    13261 cggaagacag caagctggta gaaacactca aagccgatca cgatgccaca cgccagttcg
    13321 tcagcaagcc aatcggtaaa tctgccgaca acatgtatag ctatctggcg ctggtgcagg
    13381 acgatccgac cgtgcaagta gtgaacaacg cgcaaaaagc gtatgtcgaa cattacattc
    13441 agggcgatcc ggatctggca aaactgccgg tgctttcagc tgccgcaccg tttaaagttg
    13501 gtggtcgcaa aaatgacccg gcaagctatg tggaggtgga aaaaggccag ttgaccttcc
    13561 gtaatgccgc cgatctttat ctctatccca atacgctgat tgtggtgaaa gccagcggta
    13621 aagaggtgaa agagtggctg gagtgctccg ccggacagtt taaccagatt gatcccaaca
    13681 gcacgaaacc acagtcactc atcaactggg acggtttccg cacctataac tttgatgtta
    13741 ttgatggtgt gaattatcag attgatgtta cccagcccgc ccgttatgac ggcgagtgcc
    13801 agatgattaa tgccaatgcg gaaaggatta agaacctgac ctttaatggc aagccgattg
    13861 atccgaacgc catgttcctc gttgccacca ataactatcg cgcttacggc ggcaaatttg
    13921 ccggtacggg cgacagccat atcgcttttg cttcaccgga tgagaaccgc tcggtgctgg
    13981 cagcgtggat tgctgatgag tcgaaacgtg cgggggaaat tcacccggcg gcagataaca
    14041 actggcgttt agcaccgata gctgccgata agaaactgga tatccgtttc gaaacttccc
    14101 cgtcagataa agccgcagcg tttattaaag agaaagggca gtatccgatg aataaagtcg
    14161 cgaccgatga tatcgggttt gcgatttatc aggtggattt gagtaagtaa aacacttctt
    14221 tttcggccta taaatcatca atcgcatccg gcatttattg gcggatgcga tgctggcgca
    14281 tcttatccgc cctacaagtc atacaccgta ggccgcgcat ccggcagtta tgccgcacgt
    14341 tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa tccagtccgc cagtgcagca
    14401 accttttcgc tcacctgctc gcccagcggc gtgaggctgt attcaacatg cggtggcacc
    14461 accggatacg cgatacggtt aagaaaacca tcctgttcta acgcctgtaa cgactgcgca
    14521 agcatctttt cgctcacccc gccgattttg cgccgcaggt cgctaaagcg atgagtacct
    14581 tcgcgtagcg ccaccagaat caacaccccc caacggctgg tgacgtgttt caacacctcg
    14641 cgcgacgggc actgttccgc aaagagattg ccctctttca gttgttgcga caggctaacc
    14701 tgactcattt catacttacc tttttgtatg tacttactaa aagtaagttt aggtgttagc
    14761 gtatttaaac acaagacaaa acgatggaga cttcccatga tcgctattac tggtgccact
    14821 ggccaacttg gtcactatgt tattgaatcc ttgatgaaaa cggttcctgc cagccaaata
    14881 gtggctatcg ttcgtaatcc ggcaaaagcc caggccctgg cagcacaagg cattaccgtg
    14941 cgtcaggctg actacggcga tgaagccgca ctgacatctg cacttcaggg agtggaaaaa
    15001 ctactgctga tatcttccag cgaagtgggt caacgtgccc cgcagcatcg taatgttatt
    15061 aatgccgcaa agacggctgg cgtgaaattt atcgcttata ccagcctgct acatgcagat
    15121 acctccccgc tcggcctcgc cgatgagcac attgagacgg agaaaatgtt ggctgattct
    15181 ggcatcgttt acaccctgct gcgcaacggc tggtacagcg aaaactacct cgccagcgcc
    15241 ccggcagcac tggaacacgg cgtatttatc ggtgcggcgg gcgatggcaa aatcgcctca
    15301 gcaacgcggg cagattatgc ggcagctgcg gcacgcgtga ttagcgaagt cggtcacgaa
    15361 ggcaaggttt acgaactggc gggcgatagt gcctggacgt tgacacagtt agcggcagag
    15421 ctgaccaaac agagcggaaa atccgttacc tatcaaaatc tgagcgaagt cgatttcgcc
    15481 gcggcactga aaagcgtcgg actgcccgac ggactggcgg atatgctggc ggattctgac
    15541 gttggcgcat cgaaaggcgg tctgtttgat gacagcaaaa cgcttagcaa actgattggc
    15601 cacccaacga caacgttagc cgaaagcgta agccatcttt ttaatgttaa taactagtta
    15661 attaaagtgg catcctcccg catcctctct gataatgacg ggatgccggg agcaatcatg
    15721 tctgcttcct gaactttctt ctgacagatc aatggatgcc agtaatgatt agcggcgtgc
    15781 tgtacgccct gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg ttgatcgtgc
    15841 cggaataccc ggcgatgttg cagtcgatgg ggcgttatct
//
LOCUS       DAFATA010000103        15438 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFATA010000103 DAFATA010000000
VERSION     DAFATA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.104"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..510
                     /gene="nikC"
                     /locus_tag="LGJ20_003840"
     CDS             <1..510
                     /gene="nikC"
                     /locus_tag="LGJ20_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBK2387848.1"
                     /translation="RVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARM
                     VRSLVISLRQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGM
                     SFLGLGVTAPTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLD
                     PHLVTEHAH"
     gene            510..1274
                     /gene="nikD"
                     /locus_tag="LGJ20_003841"
     CDS             510..1274
                     /gene="nikD"
                     /locus_tag="LGJ20_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBK2387849.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            1271..2077
                     /gene="nikE"
                     /locus_tag="LGJ20_003842"
     CDS             1271..2077
                     /gene="nikE"
                     /locus_tag="LGJ20_003842"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBK2387850.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            2083..2484
                     /gene="nikR"
                     /locus_tag="LGJ20_003843"
     CDS             2083..2484
                     /gene="nikR"
                     /locus_tag="LGJ20_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBK2387851.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(2604..2963)
                     /locus_tag="LGJ20_003844"
     CDS             complement(2604..2963)
                     /locus_tag="LGJ20_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK2387852.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(2960..3235)
                     /locus_tag="LGJ20_003845"
                     /pseudo
     CDS             complement(2960..3235)
                     /locus_tag="LGJ20_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(3294..4418)
                     /locus_tag="LGJ20_003846"
     CDS             complement(3294..4418)
                     /locus_tag="LGJ20_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2387853.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(4418..7153)
                     /gene="rbbA"
                     /locus_tag="LGJ20_003847"
     CDS             complement(4418..7153)
                     /gene="rbbA"
                     /locus_tag="LGJ20_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBK2387854.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(7150..8217)
                     /locus_tag="LGJ20_003848"
     CDS             complement(7150..8217)
                     /locus_tag="LGJ20_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2387855.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(8583..10205)
                     /locus_tag="LGJ20_003849"
     CDS             complement(8583..10205)
                     /locus_tag="LGJ20_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBK2387856.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALEPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(10467..12091)
                     /locus_tag="LGJ20_003850"
                     /pseudo
     CDS             complement(10467..12091)
                     /locus_tag="LGJ20_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            12475..13527
                     /locus_tag="LGJ20_003851"
     CDS             12475..13527
                     /locus_tag="LGJ20_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HBK2387857.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(13843..15045)
                     /locus_tag="LGJ20_003852"
     CDS             complement(13843..15045)
                     /locus_tag="LGJ20_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK2387858.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            15277..>15438
                     /locus_tag="LGJ20_003853"
     CDS             15277..>15438
                     /locus_tag="LGJ20_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="involved in the transport of inorganic phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBK2387859.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAV"
BASE COUNT         3849 a         3954 c         3598 g         4037 t
ORIGIN      
        1 cgcgtcgccg atatgtttat gaccttcccg acctcgattc tgtcgttctt tatggttggc
       61 gtgctcggca ccgggctgac caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg
      121 tatgcacgca tggtgcgcag cctggtgatt tcactacgcc aacgcgagtt tgtgctggcg
      181 tcacggcttt ccggtgcggg ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg
      241 atcccttcgc tgctggtgct ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg
      301 gggatgtctt tccttggcct cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt
      361 aacgacgcgc gccagtatat ctggacccag ccgctgcaaa tgttctggcc ggggctggcg
      421 ctgtttatca gcgtgatggc ctttaacctg gtgggtgacg cactgcgcga tcatctggat
      481 cctcatctgg tgacggagca cgcacactaa tgccgcaaca gattgaacta cgtaatatcg
      541 cgctacaggc cgcgcagccg ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg
      601 tgctggcgtt agtcggcggt agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg
      661 gcattttgcc cgctggcgtt cgccagacgg cgggggaaat tttagccgat ggcaaaccgg
      721 tttcgccttg cgccctgcgc ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg
      781 cctttaatcc actgcacacc atgcacaccc acgcgcggga aacctgcctg gcgttaggga
      841 aacccgccga tgacgctacg cttaccgctg ccatagaagc ggtggggctg gaaaacgccg
      901 cgcgcgtgct gaagctgtac ccgttcgaga tgagcggcgg catgttgcag cgcatgatga
      961 ttgcgatggc ggtgctgtgt gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc
     1021 tcgacgtggt agcacaggcg cgcatcctcg atctgctgga aagcattatg caaaaacaag
     1081 cgccgggaat gctgctggtc acccatgata tgggcgttgt ggcgcgtctg gcggatgacg
     1141 tggcggtgat gtcacaaggt aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg
     1201 cccccaaaca tacggtgacg cgcagcctgg tttccgctca tctcgccctc tacggtatgg
     1261 agctggcatc atgaccttac ttaacgtctg cggcctttcc catcactatg cccacggtgg
     1321 atttagcgga aaacatcaac atcaggcggt gctgaataac gtttccctga ccctgaaaag
     1381 cggcgaaacc gtcgccctgc tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct
     1441 gctggtaggc ttagagtctc cctcacaagg gaatattagc tggcgcggcg aaccgctggc
     1501 gaaactcaat cgcgcccagc gtaaagcgtt ccgccgcgat attcagatgg tatttcagga
     1561 ctccatcagc gccgtgaatc cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg
     1621 ccacctgcta tcactgaaaa aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc
     1681 ggtcgatctc gatgacagcg ttctcgacaa acgcccaccg cagttaagcg gcggccagct
     1741 ccagcgcgtc tgcctggctc gcgcgctggc ggtcgaaccg aaactactga ttctggatga
     1801 agccgtttct aaccttgatc tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct
     1861 acaacaacag tttggcaccg cctgcctgtt catcacccac gacttacgcc tggtggaacg
     1921 cttctgtcag cgggtgatgg tgatggacaa cggacaaatc gtcgaaaccc aggtggtggg
     1981 agataaatta accttttcct ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc
     2041 attccccgtg cgccgtcgca ccacagaaaa ggtttaacgc aaatgcaacg agtcaccatc
     2101 acgcttgatg acgatttact ggagacgctg gacagcctga gccagcgtcg tggttataac
     2161 aaccgttccg aagctatccg cgatattctg cgtagcgccc tggcccagga ggccactcag
     2221 cagcacggca cgcaaggttt cgcggtgctc tcctatgtgt atgaacacga aaaacgcgac
     2281 ttagccagcc gcattgtctc cacccagcac catcaccacg atctctccgt cgccacgctg
     2341 catgtgcata tcaatcacga cgactgtctg gaaatcgccg tgctgaaagg tgatatgggc
     2401 gacgtgcagc attttgccga tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg
     2461 cagtgcttgc cgaaggaaga ttgagttatt tctgaaacga ggaaagaaaa gatattaact
     2521 gaacttaatt tataaaatta agttcagttg catagataga tcctgtaaag gcagatattc
     2581 tgcgtaatgt acgccgctaa aatttataaa tgattcaaac gctcattcag tgtttcaata
     2641 atataggtat taacagagac ttgttgctgc gcagccgcat tattaaggcg ttcactcagc
     2701 gattctggat agcgtaacgt aaaggtctta atcttttctg tacgcgcata cggctcaata
     2761 cccgcagctt tacaatcctc aagatactct cgcagggaaa gttccccttc tttttgcagc
     2821 ccctgaatgc tgtcagagac aaaatcacaa taacctgaca agcccaggaa tttgccacga
     2881 aatgcattca gttctggcac gtaagtgata acagcaggtt gcccggcaat ctccatagag
     2941 tttggcgtct tcagtttgat catggtttta cccctatact tagtaaccag tcacgcacgc
     3001 tttctacagc gcctttatcg gtatctggcg acggatgagg ccgatggaaa cacgcaacgc
     3061 tcatatttag tatgaactta cagcgcgacc ccctaccttc cttaatttct ccgcctaacg
     3121 ctttaaccag agattctata tcagtccatt tgattccttg aggtaaaggt gatttaaata
     3181 gctgatccag tgtgtttttt tgtttctttc gtaaagcctg tacctgtgtt tacatgacat
     3241 tcccttatga cttcacttta tgacatcaca gtatgaagtc aagtttccct tccttacgcc
     3301 attgtcccaa tcgtcttcct gaatcgcagc agcgcgatgg tgaaaaacgc gccgccaatt
     3361 gccatcagcg tcaggaactg cggccagacg atttcgaatc cggcaccccg gtagagaatg
     3421 gcctgcgcga ggctaacaaa gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc
     3481 tgcggcatac tttcgcgcgg cgtggatcca ccggaaagca tttgcagcgg cagcagcacc
     3541 agaatcacca gcagccccag ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg
     3601 attgacgtgg tggcaaacag actgagcgcc acgcccagca taaacagcgg gatcgagcct
     3661 tcaatcggta cacccagcac gcctttcacc atcagcacca gtgataatcc cgacaccacc
     3721 agcaccacca gccccatcga ccagaccttt gccatcatga tctcaaacgg cgttatcggc
     3781 atcaccaaca agtgttccac cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc
     3841 aatacaatcg ccagcatggt aatgttgttg atgattgcca tcaccccgcc aaaccacgcg
     3901 ggatcaaggt tcgggttaaa gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta
     3961 tcacggtagc gcgcgacaaa gctgttcact tcaccgttga taatattctg gatataccca
     4021 ttgccggtaa atgcctggct catgcgcgtg gcatcgacgt tcacctgaat atccggctgg
     4081 cgtccggcga ggacatcacg ctgaaaatta ggcggaatat ttatcgcaaa ggtataacgt
     4141 ccggcgtcca gtccggcatc catctcatcg gcggtgatca tctccggtgg caaaaaccac
     4201 gggcgataga agctgttaac gatccggttc gataactgcg attgatccat atcggcaatg
     4261 gcgatcggcg cgaggttcaa cgatcctggc gtaacggtcg ctgacgaata caccgacacc
     4321 gtaaacgaga agacaatcag cgtcagcatc gctttatcac cgagcagact gcgcaactct
     4381 ttgataccca gattaaaaat attgcgtaaa tggcgcatca tccctcctgt tttttcagca
     4441 gcaggatact taagcccatc accagcggga tggctatcag caacgggata aaaagttgcc
     4501 acaaatcagt cagatccagc gctttcgaga acgttccgcg ggcgatagtc agaaaatgac
     4561 tggtcgggta aacctcgccg atccaacgtc caggcccttc cagcgaagct accggatcga
     4621 tcatcccgga aaactgtgtc gccgggatca acgtgataat cgccgttccg aaaatggcgg
     4681 caatctggct tttcataaag gtggagatca gcagccccat tccggtggca atgatgatat
     4741 acagcagcgc cgccagggtg agcgtcagga aactgccttt atgcggtacg ccaaacacaa
     4801 acaccgacag gccgcagagc aggaaaaagt tcagcatccc cagcgcgatg tatggcaact
     4861 gtttaccaag caaaaattca ctacgcgtgg tgggggtcac gtaaaggttg ataatcgacc
     4921 caagctcttt ttcccgcacc acgctaaggg cgcttagcat tgacgggatc atcatcagca
     4981 gaagcgggat caccgccgga acaatcgctg gcaggctttt tacgtccggg ttatagcgat
     5041 agcgcgtctc aatattcatc agcccgcttt ggctggcggg tgtggattgt cggctcgcca
     5101 catcctgtag ccagctctgg tgcattgcct gcacgtaacc ttttaccgtt tcggcacggc
     5161 tcggcatcgc tccgtcgatc cagacgccga gttccacagg cgtaccacgc gcgatatcgc
     5221 gcccgaaatt gggagggatc tcaattgcca ccgttatatc gcccgcacgc atccgacgat
     5281 caagctcgtc ataactggtg agcggcggct gttcgataaa gtaacgggaa ccggagagat
     5341 tgagcgtcca cgcctggcta ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt
     5401 tttccacatc catactgatg ccgtaaccca ttatcagcat caggatcact gttcccatca
     5461 gcgccagcgt cgaacgtacc ggatcgcgtc gcagttccag tgcttcgcgg cggctgtagc
     5521 taaacagacg gcgcaggcta aatccctgac gcggcgcgtg ggtggtgtcg tgtatcacgg
     5581 gcggcgcttc ggcttcgttg ctctgtcctg ccgcttcctg caaataggcg ataaatgcct
     5641 cttccagact ggcggctccg cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca
     5701 cttttccggc gtgcatcagt gagatgcggt cgcaacgttc cgcttcgttc atgaagtggg
     5761 tggagatgaa gatagtcact ttgtcctggc gcgagagatc gaccatcaac tgccagaaca
     5821 tatccctcgc caccggatcg acaccagaag taggctcatc gaggattaac atctccgggc
     5881 gatgaatcac cgccaccgcc agcgaaagcc gctggcgaat gccgagcggc aatgactccg
     5941 gcagaatatc ttcaacgtcg ttgagcttaa aacgctcgct catttcagcc actcttgcgg
     6001 gaatttccgc ttccgggatg tgaaacaaac gggcatgtaa ctcaaggttt tgccgcacgg
     6061 tgagttcgtt atagagcgaa aacgcctgcg acatatagcc cacccgacgg cgggtatcga
     6121 tatcttttgg atcaaccggt tgcccgaaca gccacgcctc accttcgctg gcgggcagca
     6181 gtccggtgag cattttcatg gtggtggatt taccgcagcc gttcgaacca aggaatccaa
     6241 aaatctcccc gcgtggaatg cggaaattaa cgtgatcaac ggcaacgaag gaaccaaaac
     6301 gcatggtcag atcgcgcgct tcgatggcaa tctctgcgtt ttcaggttga tacggtggga
     6361 tcactaccgc ctgatgcgcc tggcgttgcg cttgcggtaa cagatttata aatgcttctt
     6421 ccagcgtagc gctttgcgtt tgctgccgta gctcttcggc gctgccagtt gccagcactt
     6481 ctccggcatt catcgctacc agccagtcga agcgttcggc ctcttccata taggcggtgg
     6541 cgaccagcac gctcatattg ctctgccgct gacgaatact gtcgatcaga tcccagaact
     6601 gggcgcggga gagcgggtca acccccgttg ttggctcatc aaggatcaac agttccgggt
     6661 cgtggattaa cgcgcagcac agcccaagtt tttgcttcat cccgccggag agtttccctg
     6721 ccggacgatc gcgaaacggt gctaaccccg tgctggtcag cagctcatta attcgcactt
     6781 cccgctccgc tttgtcgtga ccgaacaggc gagcgaaaaa atcgacgttt tcatacaccg
     6841 acaaggtgtg gtagaggttt ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga
     6901 cgtcgcggcg atgcctcggg tcgcgcatat cgccgcccag caccatcaca ttgccctgct
     6961 caatgacgcg ggcaccggaa atcaacgaca ataagctcga cttcccgacg ccatccggac
     7021 caatcagccc gaccatacag cgggccggaa tatcgagagt aatattgttc agcgcaacgg
     7081 tttttccata atgctggctc acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt
     7141 ccagatgcgt cattgcggca acctcaccac gaggtcgtca ggccacggaa gttcttcatt
     7201 cacccgcacc cacgctacgc ccggtaaacc ggttttgaca tattccagat gctgctggag
     7261 taattccggt gggatacgcg ctttgacgcg gaacatcagt ttcagccgtt catcgctggt
     7321 ttcgacggtt tttggcgtga actgggcgac actggcgaca aaactgatgg tcgccggaat
     7381 acgcagatct ggcgcggcat cgaggatcag ccgggcttca ccacccagtt tcagcgtgcc
     7441 cgcctgttcg gttggcagga agaaggtcat atagacgtcg ctgagatcga ccatattcag
     7501 cacccgaccg cctgccgcca gcacttcccc tggctcggca acccgatact gcacgcgtcc
     7561 gtcacgcggg gctttcagtt cgctgtcatc gatatctgcg gcaatgcgcc gttcagtggc
     7621 ttgtgccgct tcgacgcggg tttgcgcctg aatgatattg gtacgtgccg cctctatagc
     7681 ggctttagaa gcagaaacct gagcttttgc cgattccagc gcagctcggg cgctctcagc
     7741 ggcggcgcgg tcgtcatcca gctgttgcgc agaaatagcc cctcgttggg ccagtgaacg
     7801 ggaacgcata tgacgttttg ctacggagtc cagttctgcc tggcgttgat taaccagcga
     7861 ctgcgcggcg cgagtttcgc tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc
     7921 gctttgtgcc tctttgattt gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt
     7981 atccatcttc gccagcactt caccttcgcg aacaaactgg ccttctttca ccagaatggt
     8041 gtcgatacgc ccggcaattt tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt
     8101 gctgacagca aagccttccg gcacacccgc cgggcgcaac agccaccacg ccacgacggc
     8161 agccaccgcc agtgccccga caacccacca cgccagatgg cgcttactct tatccataat
     8221 cggcccgcca taatccctgt aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt
     8281 cagcggattc ttccatccgg gataagaagg aaacgacaaa tacgaccagc gcgcagagca
     8341 ttgagcgcca acggggaagg cttagtgaaa acgggtagta atgttgtcat catcagcatt
     8401 cctggccgta aatgaaacac gcatactcat aagtgtgatc tataaaactg gctgacaaaa
     8461 cggtatccat cctgttggat cgcgtttcac gcctgttcag ttgcaggaac agagcattcc
     8521 tttttacgtt ttatcaacag tctcaaccag atagtaacat taatattcat gttgtctatg
     8581 gttcagagct gtaacattgt gcgccttcta atcaaaggca taagtccatt tctgtttttt
     8641 tcatgctggt aagacttcca gggagtggtt atctcaggcc ctgcaccagg gttaatttgg
     8701 caattatgca tgctgaacat tcttccagca tgtttactgt cacgggcgtt aagacctaaa
     8761 actgtccccg tttcacctat acattctctt ccaccattac cgttatgtgc aacaacttga
     8821 tttataccta acatttgtga gcatgctaat agtgcttcaa gtttaggtct gtaatctgtc
     8881 gaaacagaaa aataacgatt aaaggatcga caagagatag cccggctatt tttaaacttt
     8941 ttgccattaa ttacagcatg gtgcttttta ttgataattg ccgctaattc gaccggttcg
     9001 agagcattgg tcatttcatc actagagact gttatcggtc ctataatgta acgttttcgc
     9061 tgatcatcaa aagttatccc atgatgattt gccattattt tgtatttggg gccatagaaa
     9121 catacaggat aatcttttat cccagcgtag gtatcctcat caagtggttt catgcgcgtg
     9181 aagttctgca tatagttacc attgcaattc gtttcatgat ttccagctaa tacatgaaca
     9241 ttttcattga tactcattat gcgttttaac aaatcgatga taaattgatc gccacagata
     9301 ttactaaatc gatcgccggt ttggtcgcca agaaacgtac atggcgtcac agcgtcttta
     9361 ttaataacta aataaggctg aagactttct aaaactatat ctcttctgtt cagaatactg
     9421 attattgtat taacattagt gtcaagttgc tcattaaagt tctcccttag caatacgttg
     9481 agaacttcat cttctatatt aagcaactgg ctaaggaaat tttctccttc agccatgatt
     9541 ctcaaatgtc ccgttgcaaa aagcgcgtac aaaactgcac cgactgagcc atcggtatcg
     9601 ccaaaatagg ttggtctggt acttgtggga tcaacaggtg aacaattgga gaaataggtt
     9661 ctgtcagcga actgggggtt tatcattgtt tcgaatttaa tatcagatac attgaattgc
     9721 gatattttcc agtcccagac gggtttgtac gactcagtaa ctgcaacatc attagcatca
     9781 tcatttactg tcatattatt aagtaaataa acaatgctgc accaattaat atcggtatta
     9841 tcaagatagc agccgtaatt tttactatta attgtcacaa taaactgata attattatcc
     9901 ctacgtagaa tatgagcgcg catgtccgat agcatactat atttttctga atgattcgtt
     9961 tgtatggtaa atgatatttt gtcatctcga atagattgtt ggaaacgttc tatttgccaa
    10021 tcatcaattt tttccacaac ttcaccgagc attgtcctgg caatacttga tatatcatca
    10081 cctataccat ttgcaatata cccaaacagt tctttagtga agttagtaac atgctcttgc
    10141 accatatcat ttgttgcgat tgtcgttgta ctgtcgttag tgcctgccac agttccaatt
    10201 ttcatctttt acctcatata attcaccgcg aggaatgcat agttaacatt taatcttatg
    10261 acatacacct catgttcgat ggaaaaataa ttatattttg cacaaatcat tcagccttaa
    10321 agcataatat gaaatttctt atctcatagc agagaaagtc cctaaagatt gtaaaggcgt
    10381 catcagaaca tgcctctaca atcgattaag aaaacctatg aaatactggc aggattaaaa
    10441 agacatacca ccgtattcat ggagttttaa tgagattgaa aacaactaag ccctgaggaa
    10501 taaattattt tattattttt atcacgtgct tccttcaggg aatttagccc cataatacca
    10561 ttatcaaatt gttgattctg atctaccccg accccatgat gcccatattt ttgtctaatg
    10621 tatttgctgg ggtcttcacc tttgagtgtc tccccatata tgaagatacg tttacgtgct
    10681 ggtctgaaat cagtagtgtt atcaaaataa ttagcatata caggtgtggg tttcaaagta
    10741 ttgcttcgga ttagatggaa cagaggatca tccatgtgtt ctttatgttt tttattaaaa
    10801 atattggcta attcctcagg atcagtagga tttttcatct gattaaatgg aacctgcaaa
    10861 gcaccgagac aataacattt ttttcttcat ctcttattat tccgtgatga ctggttaaaa
    10921 ctttcctttc tgagtcataa ttacaaacat cgagcgtttt aattaagtta tacgtatcac
    10981 cggaagagag tttatgattt gctagtcgtg cactatagtt accattaaaa ttgatttcat
    11041 gatttccagc cagaacgacg acatttttat ttatacgact atctttatta ccctccatat
    11101 ttcgcataga atttagcaaa gtaaggatat atttatcacc aaaaatagta ctgaatctat
    11161 cgcctgtatg atccccaagg aagatacaag gggtaatcgc atcgcctttt aaaaaaacat
    11221 gttgatgcaa ctcattcaat atcatgtctc tcgtatttaa taaattgtag agcaatttat
    11281 tatttttatg tatgtatgca gatgaagcca tgttttcgtg ttttaataat tcacataata
    11341 aactccaacc cttttccctg atgcctatat gccctgaaac gagcaatgca taaagtacag
    11401 cgcctactct tccatctgta tcgccaaaat aagccgtgtt ggcattttgt gggttaatca
    11461 caccacattg tttataaaag tctattttag aaaacacaga tttaacatag ctatcaaact
    11521 tatccttagg aaacgtctct ttagagacat cccaacgcca gtcagcttta aaattaccgc
    11581 taagaagagt aaagaaattt tcaggtatga tagaaagccc attaacttta taagttatgt
    11641 ctgatttata aattatgtct gatttatctc cggagaaatt aataaaataa taaccatcct
    11701 tacactcaat cttaactgta atgggagacg ataattttat cacatggtta ttgtctggta
    11761 taattgtaaa gataaacgtg ttagtagtat tatcggcgaa actattatat tcttgaatat
    11821 ctatagatgg ctcactattg taaaaccata ttttctccat ctgaagacta tgttcattta
    11881 ccctccagtg accatgttgc aggaaataga tgatattcag gcctaaatcg ttatctattt
    11941 gcgaaaagtc attcagcata ttaacttcaa ctgtgtttga tcgatatttt ttatcaacct
    12001 tgccatggtg ttgatgactg ttaactttat ctgaatgctt atcttgatgg tttgaagaga
    12061 agtgcttaaa tatattgtga aatactttca taaataacct gtaatatttt tacttgttag
    12121 taacaaaata aacaatacat gtctgaaaga catatttacg atgatacaaa gcaataatac
    12181 ttatatgttc gatgatttta agcaccgaga ctatccggtc ttttactaaa taatggaaaa
    12241 caatccatcc ctggaagata attgtaaaaa aaacaacaga ataagaatta atcttaggat
    12301 aaatatttat ttatcatggc cttttaaccg gtaaaaaatg gcactcggca cagtaaaccc
    12361 cgctatagtt atgctatgca tttgtcttta atgatatctc gtcgttatat taacaggctg
    12421 aaattatcat taatgcacat ttcattatta gcagggatac ctgaggagtt taaagtgaac
    12481 atatatatcg ggtggctttt caaattaatc cctttgatta tgggattaat ttgcatcgcg
    12541 ttaggtggct ttgtgctgga aagttcaggg caaagcgagt atttcgtcgc gggtcatgtg
    12601 ctgatttctc tggcggccat atgcctggca ttattcacta ccgcatttat tatcatttcg
    12661 cagctcacgc gcggcgttaa tacgttttac aatacattgt tccccattat tggctatgcg
    12721 gggtcaatta tcaccatgat atggggttgg gcactgttag caggcaatga tgtgatggca
    12781 gacgagtttg tcgccggcca tgttattttc ggcgttggta tgattgccgc ctgtgtatcg
    12841 acggtggcag cgtcatccgg tcactttctg ctcattccca aaaatgcagc ggggagcaag
    12901 agcgatggaa cgccggtaca ggcttattct tcattaatcg gtaattgcct cattgccgtt
    12961 cccgttttac tcaccctgct cggtttcatt tggtctatta cgctgttacg tagtgctgac
    13021 ataactccgc attatgtcgc gggtcacgta ttgcttgggt taaccgcaat ctgtgcctgt
    13081 ctaattggcc ttgttgccac aattgtccat caaacacgta atacgttttc aactaaagaa
    13141 cactggctgt ggtgttattg ggttattttt ctcggctcaa tcacggtact gcaggggata
    13201 tacgtcttag tcagttccga tgcaagcgcc cgactggctc ccggcattat tcttatttgc
    13261 ctcggaatga tctgttacag catattctca aaagtctggc tactggcact ggtatggaga
    13321 cgtacctgtt cgttagccaa cagaataccg atgattcccg tcttcacctg cctgttttgc
    13381 cttttcctgg catcgtttct tgcggaaatg gcgcagaccg acatgggata ttttattcct
    13441 tcgcgggttt tggtcggttt aggagcggta tgctttacgt tgttctcaat cgtttcaata
    13501 ttagaagcag gttctgctaa aaaataattg caacgtaccg gataaaacca gcgttgacca
    13561 tttgcgtaac gctggttttt cttaggcatc atgaaataac gcagcattaa tgcatagtgg
    13621 ttaaggataa aaaaacaaca aagttactgt ttttttcaac ctgttcatat tttataaaga
    13681 tctggccact gggtgagttt tcaatcacct ttccttccac cttatattaa gcctggaggg
    13741 tttcagttca cgggctcatt agaaaataat cacaagatag ccactatcaa aattagctca
    13801 tttttaatgc ggctctaata attttcatct ttaggtaaca ggtcaggacg acttcgctgc
    13861 aatcaaatcc tgcgcacaag cccacgcact cgaccacgcc cactggaagt tatagccccc
    13921 cagccagccg gtaacgtcca tcacttcgcc gatgaagtac agcccaggca ctttgcgcgc
    13981 ttccatcgtc cgtgaagaga gttcgttggt gtccacgccg ccgagcgtca cttcggcagt
    14041 gcgatagcct tcagtgccgt tgggttgtac gcgccagtcg gtcaatgtgc taatcagtgc
    14101 ctgttggtca cgcacgttga gctgttttag cgaaacatcc gggatttgcc cgagttgctg
    14161 taagcgttca accaaccgct tcggtagatg aaccgccagt gtgtttttca ggctctgatt
    14221 cggatgtgcg ttacgctgct cattcaggaa ggtttcgaga tccacatccg gtagcagatt
    14281 gatactgaca aattcacccg gttgccagta gctggaaatc tgcaacaccg ccggtccaga
    14341 caagccgcga tgggtgaaga gtaagttctc acggaaaacg atgccgtttt cagcggtaat
    14401 cacggaaggc accgccacgc ccgccagcac ctgtaactct tcgagcaacg gtttatgcag
    14461 agtgaatggc accagacccg cgcgggtcgg cagcacgttg aggccaaatt gttcggcaat
    14521 cttataacca aacggcgacg cgcccagccc cggcattgac agcccaccgg tcgcgatgac
    14581 cagcttttcg caaccgacag tcatgccgtt cagttcaagc gtgaagcctg tttcatcctt
    14641 cgccacactc agcacttcgc tacgcaatct gaaggttaca ttgcccttct cgcactcatc
    14701 caccagcatg tcgacaatct gctgcgcgga gtcatcgcag aagagttgtc ctaacgtttt
    14761 ctcgtgccag gcgatgccgt gtttattgac cagatcaatg aaatcccact gggtaaaacg
    14821 cgcgagtgca gacttacaaa aatgcggatt ctggctcaga taagcgcctg gttcgacata
    14881 aaggttggta aagttgcagc gcccaccgcc agacataagg attttgcgcc ctggtttttt
    14941 accattatcg atcagcagaa cccggcgtcc tgcctgacct gccagcgcag aacagaacat
    15001 acccgccgca ccagcgccta taataatggc atcaaacctt tccacgttgc gctcctctta
    15061 gaaaaaacgg gcgtgaattg taaagattcc tcagtggtcg caccagcatc aatattacta
    15121 aaaggaagta tttgcctgaa ttatataaga taattatttt ttgagtgaaa tccatacagg
    15181 aggcaaatca aaaaaagtct atatttcact ttgcccgcgc cgcgaaagtc actgataatg
    15241 cgccgcgttc atgtcctcaa aatggcgtaa cgtcctatgc tacatttgtt tgctggcctg
    15301 gatttgcata ccgggctgtt attattgctt gcactggctt ttgtgctgtt ctacgaagcc
    15361 atcaatggtt tccatgacac agccaacgcc gtggcaaccg ttatctatac ccgcgcgatg
    15421 cgttctcagc tcgccgtg
//
LOCUS       DAFATA010000104        15217 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFATA010000104 DAFATA010000000
VERSION     DAFATA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.105"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(194..1867)
                     /gene="rpsA"
                     /locus_tag="LGJ20_003854"
     CDS             complement(194..1867)
                     /gene="rpsA"
                     /locus_tag="LGJ20_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK2387860.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(1978..2661)
                     /gene="cmk"
                     /locus_tag="LGJ20_003855"
     CDS             complement(1978..2661)
                     /gene="cmk"
                     /locus_tag="LGJ20_003855"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK2387861.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(2834..3598)
                     /gene="ycaL"
                     /locus_tag="LGJ20_003856"
     CDS             complement(2834..3598)
                     /gene="ycaL"
                     /locus_tag="LGJ20_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBK2387862.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(3767..5050)
                     /gene="aroA"
                     /locus_tag="LGJ20_003857"
     CDS             complement(3767..5050)
                     /gene="aroA"
                     /locus_tag="LGJ20_003857"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK2387863.1"
                     /translation="MESLTLQPIASVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(5121..6209)
                     /gene="serC"
                     /locus_tag="LGJ20_003858"
     CDS             complement(5121..6209)
                     /gene="serC"
                     /locus_tag="LGJ20_003858"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBK2387864.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(6408..7100)
                     /locus_tag="LGJ20_003859"
     CDS             complement(6408..7100)
                     /locus_tag="LGJ20_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK2387865.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            7230..8990
                     /locus_tag="LGJ20_003860"
     CDS             7230..8990
                     /locus_tag="LGJ20_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBK2387866.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            9396..10253
                     /gene="focA"
                     /locus_tag="LGJ20_003861"
     CDS             9396..10253
                     /gene="focA"
                     /locus_tag="LGJ20_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBK2387867.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            10308..12590
                     /gene="pflB"
                     /locus_tag="LGJ20_003862"
     CDS             10308..12590
                     /gene="pflB"
                     /locus_tag="LGJ20_003862"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK2387868.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            12782..13522
                     /gene="pflA"
                     /locus_tag="LGJ20_003863"
     CDS             12782..13522
                     /gene="pflA"
                     /locus_tag="LGJ20_003863"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBK2387869.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(13604..14194)
                     /locus_tag="LGJ20_003864"
     CDS             complement(13604..14194)
                     /locus_tag="LGJ20_003864"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HBK2387870.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQIGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            14294..15202
                     /locus_tag="LGJ20_003865"
     CDS             14294..15202
                     /locus_tag="LGJ20_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2387871.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYEQLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         3848 a         3861 c         3567 g         3941 t
ORIGIN      
        1 tggcaagtct ttctatcaat tctgacttgg tcatgattcc tccggttcct taaaggcaaa
       61 ttaagcggct gctgttgctg cattgattaa gggcggccgt agccgccctt agtgcttgat
      121 tacaggacga aacctgcaat ctgtcaagta aactcaacaa acttcggaat aaaaatcccg
      181 aagagtcaga gaattactcg cctttagctg ctttgaaagc ttcagccatt gcgttgttgg
      241 agaagtttgc atcttcctgt ttgttaacag ttgcgattgc atctttctcg tcagcttcgt
      301 ctttcgcacg aacagacagg ctgattgcgc ggtttttacg atcaacgccg gtgaatttag
      361 cttcaacttc gtcgccaacg ctcagaacca gggtagcgtc ttcaacgcgg tcacgggatg
      421 cttcagaagc acgcaggtaa ccttcaacgc cgtcagccag ttctacggtt gcgcctttag
      481 cgtcaactgc agttacttta ccggttacga tagcgccttt cttgttcaga gcaacccagt
      541 tgttgaacgg atcttctgcg agctgtttaa cgcccaggga gatacgttca cgttctgcgt
      601 caacctgcag aacaactgca gcgatttcgt cgcctttttt gtattcacga actgcttctt
      661 cgcctgcaac gttccaggag atgtcagaca ggtgaaccag gccgtcgatg ccgccgtcca
      721 ggccgatgaa gataccgaag tcagtgatag acttgatttt accttcaaca cggtcgccct
      781 tgttgtgggt ttccgcgaac tgctgccacg ggttagcttt gcactgtttc agacccaggg
      841 agatacgacg acgttcttcg tcgatatcca gaaccataac ttccactaca tcgccaacgt
      901 taacaacttt ggacgggtgg atgtttttgt tggtccaatc catttcggaa acgtgtacca
      961 ggccttcaac gccttcttcg atttcaacga agcagccgta gtcggtcagg ttggtcacgc
     1021 gaccagtcag tttggtacct tccggataac gtttagcgat agctacccac ggatcttcgc
     1081 ccaactgttt caggcccagg gatacacggg tacgttcgcg gtcgaacttc agcactttaa
     1141 cagtgatttc gtcgcccacg ttgacgattt cgctcggatg cttaacgcgt ttccaggcca
     1201 tgtcagtgat gtgcagcagg ccgtcaacgc cgcccagatc aacgaatgca ccgtagtcag
     1261 tgaggttctt aacgatacct ttaacttcca tgccttcctg caggttttcc agcagctgat
     1321 cgcgctctgc gctgttttcg gattcgataa cggcacgacg agaaacaaca acgttgttgc
     1381 gcttctgatc cagcttgatt actttaaatt caagctcttt gccttccagg tgcagagtgt
     1441 cacgcaccgg acgaacgtct accagagaac ctggcaggaa cgcacgaata ccgttcagct
     1501 caacagtgaa gccgccctta actttgccgt tgataacacc ggtaacagtt tcagcatctt
     1561 cgtaagcttt ttccagcgtg atccaggctt cgtgacgttt agctttctca cgggacagca
     1621 gagtttcacc gaagccgtct tctactgcgt ccagagcaac gtcaacttcg tcacctacct
     1681 ggatttccag ctcgccctgg gcgtttttga actgctcagc cgggatggcg gactcagatt
     1741 tcagaccagc gtcaaccagt actacgtctt tgtcgatagc aacaacaacg ccacgaacga
     1801 tagaacccgg gcgggtttcg atttctttta aggactcttc aaagagttga gcaaaagatt
     1861 cagtcatgtt taatcttcag gtttatattt aacgtccacc tggctccatg ccggatgggg
     1921 ttgtttaaca tacccgctgt cattccattg caacgggggt actgcaaatt cggtcgctta
     1981 tgcgagagcc aatttctggc gcgcgtattg tagcgctttt tcaatcactt gctcaatgct
     2041 taaggtggtg gaatccaaca ctaaagcatc ggctgccgga accagtggcg ctaccgctcg
     2101 gttacgatcg cggtcgtcgc gttctttgat ctcggccaaa aggcgctcaa agttaacact
     2161 aaagcccttc tcctgcaact gtagcatgcg gcgatgcgca cgttcttccg aggaggcgtc
     2221 aaggaaaatt ttcactggcg catcagggaa taccaccgtt cccatgtcgc ggccatcggc
     2281 aatcaggcct ggtaattcgc gaaacgcgcg ttggcgacgc aataatgctt cacgaacgcg
     2341 tgggaatgcc gcgacttgtg aagctgcatt cgccacttcc tgagtacgaa tttcgccgct
     2401 gacatcttcc ccttcgagga tcacttccag attgccattg gttgacacaa aacgtacatc
     2461 cagatgggat gccagcggta ccagcgcatc ttctgacgca acatcaacat ggtgatgtaa
     2521 tgccgccaat gccagtacgc gataaattgc acccgaatcc agcagatgcc attgcaatgc
     2581 ttccgccata gccttacaca aggtgccttt ccctgcaccg cttgggccat caatggtaat
     2641 aaccggggca attgccgtca tctttatctc cttaaaacag gcgtaccgtt aacataaccg
     2701 cgcgcattat acgcgccgac gccacaaatc gttaacgttg tgtgcaaaaa ccagttttgt
     2761 ctgtattcag atggggattt attgcgcaat tatagcgggc tggcagaaga ccagcccgaa
     2821 agatgacaat gatttactta ccagaggcga tacgatcacg gatgtgttgc gcacgctctg
     2881 ttgatggtgg gtgagagtca aacatggact gggtgcgacc gccatccaga ctagccagtt
     2941 tttcaaagct gccaaccagc ccctgggtgc tgatgccacg tttcttcaac agatcaaagg
     3001 agaaatcatc tgcttcggac tctttatcac gggagtattt agcattaata gcgccttctg
     3061 cgatatcacc caattgtgag cgggaaagct gggaagccac tccgctggta gctgaaatgg
     3121 catcgcgtgc ggcaacgatc gcataagaag ctttcatttc agccagcgag tgacccaacg
     3181 cgacgtggcc cagttcatgg cccagaacac cttcaatttc gttgtcattc atcatgtcca
     3241 tcaggccact gtagacacgg acacagccgt tcgccatcgc ccatgcgttg acgtcgctgg
     3301 tcatataaac cttatagttg accggcgtgc cattaatatt gttacccaat gctttggcga
     3361 tttttgccag acgtttaccg tacttgctgg atttacttgc gacttgattg ccgctgtcca
     3421 tttcggcaca gccctgattg gcaatcgctt ttgcatcggc atcgcttaat gttgctgctt
     3481 tataggcatt taaaccggag ctgatagcca tattggtatc aataccgtgg gtattttgac
     3541 accctgtcag taatgctgca gaggtcgcaa tcgccagcag taatttagta ttcttcatgt
     3601 gaatcatccg ttgattaatc atatataaat attatgtatc aacaacttac attgacaagc
     3661 gacgctacca ttaatcaaaa tgactcacaa ggtccgaaaa aaaacgccga caatgtgccg
     3721 acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag gctgcctggc
     3781 taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat ttgggatcaa
     3841 gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc atccggtgat
     3901 cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata cgaatgtaat
     3961 cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc gcaaacaggc
     4021 gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg ccttttgcaa
     4081 ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata tccatatcaa
     4141 tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa atggtcgcgc
     4201 ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta cgtccaatac
     4261 cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa gaagccgaag
     4321 atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg ccttttacga
     4381 caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc agattgagtg
     4441 tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc gtatcttccg
     4501 gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg gagccatcaa
     4561 cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa ttttcttgtt
     4621 ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga ccaatcgggc
     4681 gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga caaagagctg
     4741 ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg gcaccttctg
     4801 cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct gaaagcgtat
     4861 agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg ctatccagca
     4921 gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg cggttagaaa
     4981 cgctcttgga accgggcaga ttaatagtgc catcgacact agcgatgggt tgtaacgtca
     5041 gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc tggctgtggg
     5101 gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat gaagtctgtc
     5161 agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat tccgccgacc
     5221 acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag gaacaatttg
     5281 tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt agctttcgcc
     5341 acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc tgctttttgc
     5401 tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt aaagaccaga
     5461 ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt atcgttgagg
     5521 atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa atcttcacga
     5581 acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc gtaaatcacg
     5641 ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc ggcggcgacc
     5701 accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat ggtttcattc
     5761 gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat tggcttaacc
     5821 gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca gtattttttc
     5881 gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc ggtggtttta
     5941 tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc atggcagaat
     6001 aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc ggcttcctct
     6061 gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt accaagaccg
     6121 ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag cattgccgga
     6181 ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt gctattaacc
     6241 cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc ggttttatca
     6301 ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg cttgcatcac
     6361 acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca ggtcacccgt
     6421 tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa ttgtttttcc
     6481 cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc cgactccggc
     6541 accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg tttcaccttg
     6601 tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc cagacgtacc
     6661 tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa ttccgtcatg
     6721 ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac aaccggctta
     6781 ccttccagta aatcttccag tttttcactg tgcgccatca accacattac caggcggtat
     6841 aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc atcataaaac
     6901 gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa cagcgacatc
     6961 tgtcgcacac cgcgtcttcc ggtcattttg aggaacagaa aaaccagtac aaaggtataa
     7021 agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa tgccatacgg
     7081 tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta aaagcatagt
     7141 caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc gtattattgc
     7201 gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc ggcaaagatg
     7261 ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc ggctttcaga
     7321 ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat attcgcgaca
     7381 aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg gcgctggctt
     7441 ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct gatttctggc
     7501 tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa tggttcccac
     7561 tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc gcgttttatg
     7621 atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt aacgaagatc
     7681 gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt tatattccga
     7741 tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat acccgcaacg
     7801 aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac cgcattattg
     7861 ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac ccagcagtag
     7921 ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat gacggttcgc
     7981 ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac ggtacctgct
     8041 ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc gtgaccgagc
     8101 tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc ttcgatgatg
     8161 aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc ggtttaatct
     8221 cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat ttctccggca
     8281 ccacggaaga agagttcgcc acactgatgg ctatcttcaa caaagaagat aaagaagttt
     8341 atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg cctggcatgt
     8401 ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc agccatttac
     8461 gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac ctgaacctca
     8521 tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag ctgttgggtc
     8581 tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta aaagccatgc
     8641 tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg acgatggagt
     8701 ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg caaacgttgt
     8761 tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg tttgttcgca
     8821 tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa gcggcgtttt
     8881 acggcctgca accggtagat agcgatctgc acgcgtttgc tgcgcatcag tcgttgttga
     8941 aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa aatcaaatag
     9001 cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt tttttaatgc
     9061 cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta aatatcggta
     9121 atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc tcccttttaa
     9181 ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt tttaaaattt
     9241 atttttattt ggataatcaa atatttactc cgtatttgca taaaaaccat gcgagttacg
     9301 ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat gctttgttag
     9361 tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac ccttttgatc
     9421 ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat aaagcaacga
     9481 aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc tcaatcgcat
     9541 tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc atggcaaaac
     9601 tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc ggagccgatc
     9661 tctttacttc cacagtgttg attgttgttg ctaaggcgag tgggcgcatc acctggggtc
     9721 agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca ctgctgtttg
     9781 tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg ggactaaacg
     9841 tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc tgccttggta
     9901 tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc cgtagcctga
     9961 tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc ggttttgagc
    10021 acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac ttcgcatctc
    10081 cggaattctg gaccgctgtc ggttctgcac cggaaaattt ttctcacctg accgtgatga
    10141 acttcatcac tgataacctg attccggtta cgatcggtaa tattatcggc ggtggtttgt
    10201 tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgatcaccat taatggttgt
    10261 cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg tccgagctta
    10321 atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag aatgaagtaa
    10381 acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag tccttcctgg
    10441 ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc gttaaactgg
    10501 aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc atcacctctc
    10561 acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag actgaagctc
    10621 cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt tcctgcaaag
    10681 cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac cgtaaaactc
    10741 acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt aaatctggtg
    10801 ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac taccgtcgcg
    10861 ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc acttctctgc
    10921 aggctgatct ggaaaacggc gtaaacctgg aacagactat ccgtctgcgc gaagaaatcg
    10981 ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac ggctacgaca
    11041 tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc ggctacctgg
    11101 ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc accttcctgg
    11161 atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa gcgcaggaaa
    11221 tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact ccggaatacg
    11281 atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt atgggcctcg
    11341 acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg tacaccatgg
    11401 gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg ctgaacttca
    11461 agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag aacgatgacc
    11521 tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta agcccgatga
    11581 tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa accatgctgt
    11641 acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg aagtctgaac
    11701 cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat cacttcatgg
    11761 actggctggc taaacagtac atcactgcac tgaacatcat ccactacatg cacgacaagt
    11821 acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc accatggcgt
    11881 gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa tatgcgaaag
    11941 ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa ggcgaatacc
    12001 cgcagtttgg taacaatgat ccgcgtgtag atgacctggc tgttgacctg gtagaacgtt
    12061 tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact cagtctgttc
    12121 tgaccatcac ttctaacgtt gtgtatggta agaaaactgg taacacccca gacggtcgtc
    12181 gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac cagaaaggtg
    12241 ctgtagcgtc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa gatggtatct
    12301 cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt cgtaagacca
    12361 acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa ggtggtcagc
    12421 acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa aacccggaaa
    12481 aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac tcgctgacta
    12541 aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa ttagatttga
    12601 ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg ccttttttag
    12661 cacgagagcc ttttttgtca gctatctata ctttaaggtg actgccaaaa cagactcgac
    12721 gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga gaaacaccgc
    12781 aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag acggcccagg
    12841 tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt gtcataaccg
    12901 cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa tgaaagaggt
    12961 ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat ccggcggtga
    13021 ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa aagaaggcat
    13081 tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga ttgatgaact
    13141 gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg agatccacca
    13201 aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc tggcgaacaa
    13261 aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg atgacgattc
    13321 agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa tcgagcttct
    13381 cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt acaaactcga
    13441 cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc ttgagcagta
    13501 cggtcataag gtaatgttct aatttccccg taaagcggca actttattga gttgccgctt
    13561 ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt gcatctacca
    13621 tatcccgtac ctgagataat aacgactgat aatagctcgc gtgaagttct tccccatcga
    13681 ataccactgt attacacaag aaagtgacat cggcatcttc aataccaaga taactgcaca
    13741 tattttttaa ataatcgctt atattttttt cccagcctat ctggacaaat gattctttgt
    13801 ctcctccaac cagcgccacc caacgaactt tattgaatgg taatttgtgc ccatctccat
    13861 aagccagccc attattccat actctgtcaa tatatccttt tagcattgcc gggaagctgt
    13921 accaccagag aggaaaaacc accactaacg tgtcatgctc aagcagctct gaataaagct
    13981 gatgaacctc tggagaataa cgtttatcca tattcttcca gtctggttca tcttccgacg
    14041 tcatcaccgg gttgaaatta cgccgatata aatcgagttc cgtcacctgt attttccgct
    14101 ccattgcccg ctgatggatc gcatcagcaa tatgtgcggt caatgaatca tgacgaggat
    14161 gggcccatac caaataaata cgttcagact gcataatttt acctccacgt taagttctgg
    14221 agcgcacttt acgcaggcgg cataatcctg taaacgtgat gattcaggat gagttaatga
    14281 aggattttca ctaatgcgga tgaatatgtc tgactttgcc actttctttg ccgtggcccg
    14341 taatcaaagc tttcgtgcag cgggcgatga attaggctta tcctcgtccg ccattagcca
    14401 tagtattaaa acactggaac aacgtcttaa aattcgctta ttcaatcgga caacccgaag
    14461 cgtatcgtta acggaagcgg gttctaatct ctatgaacaa ttgcgcccag ctttcgacga
    14521 aattcagatc atgttagatg agatgaatga ttttcgtctg acaccgacag gaacgctcaa
    14581 aatcaatgcc gcccgggtag ctgcacgtat atttttaatg cctttactgg ttggtttcac
    14641 acgtgaatat cctgatatta aagtcgagct cacaactgac gactctcttg tcgatatcgt
    14701 ccaacagggc tttgacgctg gcgttcgact tagtggcatc gtcgaaaaag atatgatcag
    14761 cgtggcgatc ggcccgccag tgaaattatg tgtagcagca acgccagagt actttgcaag
    14821 atatggcaaa ccccgccatc ctcatgatct gcttaatcat caatgtgttg tttttcgtta
    14881 tcccagcggc aaacctttcc actggcaatt tgcaaaggag ctggaaattg ctgtcgcagg
    14941 aaatatcatt ctggatgatg ttgatgctga actggaggcc gttttgatgg gtgctggtat
    15001 cgggtatctg ctctatgaac agatcaagga gtaccttgat actggtcgtt tagaatgtgt
    15061 actggaagac tggagcacag agcgtccagg cttccagatc tattatccaa accgccagta
    15121 tatgtcctgc ggcttgcgcg catttctcga ttatgtaaaa acgggtcaga tctgccagag
    15181 tcagcgtcac cgaccacaat aattatttac tattcgc
//
LOCUS       DAFATA010000105        14845 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFATA010000105 DAFATA010000000
VERSION     DAFATA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.106"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(125..1435)
                     /locus_tag="LGJ20_003866"
     CDS             complement(125..1435)
                     /locus_tag="LGJ20_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBK2387872.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(1463..2794)
                     /locus_tag="LGJ20_003867"
     CDS             complement(1463..2794)
                     /locus_tag="LGJ20_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBK2387873.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(3069..4433)
                     /gene="tdcG"
                     /locus_tag="LGJ20_003868"
     CDS             complement(3069..4433)
                     /gene="tdcG"
                     /locus_tag="LGJ20_003868"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBK2387874.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(4505..4894)
                     /locus_tag="LGJ20_003869"
     CDS             complement(4505..4894)
                     /locus_tag="LGJ20_003869"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBK2387875.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(4908..7202)
                     /gene="tdcE"
                     /locus_tag="LGJ20_003870"
     CDS             complement(4908..7202)
                     /gene="tdcE"
                     /locus_tag="LGJ20_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBK2387876.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(7236..8444)
                     /gene="tdcD"
                     /locus_tag="LGJ20_003871"
     CDS             complement(7236..8444)
                     /gene="tdcD"
                     /locus_tag="LGJ20_003871"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBK2387877.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(8470..9801)
                     /gene="tdcC"
                     /locus_tag="LGJ20_003872"
     CDS             complement(8470..9801)
                     /gene="tdcC"
                     /locus_tag="LGJ20_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBK2387878.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(9823..10812)
                     /gene="tdcB"
                     /locus_tag="LGJ20_003873"
     CDS             complement(9823..10812)
                     /gene="tdcB"
                     /locus_tag="LGJ20_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBK2387879.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(10911..11849)
                     /gene="tdcA"
                     /locus_tag="LGJ20_003874"
     CDS             complement(10911..11849)
                     /gene="tdcA"
                     /locus_tag="LGJ20_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBK2387880.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            12038..12382
                     /gene="tdcR"
                     /locus_tag="LGJ20_003875"
     CDS             12038..12382
                     /gene="tdcR"
                     /locus_tag="LGJ20_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBK2387881.1"
                     /translation="MTGITIFYGDNIIRYVVNIKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            12638..13177
                     /locus_tag="LGJ20_003876"
     CDS             12638..13177
                     /locus_tag="LGJ20_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387882.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPTHFNKTCIT
                     SDYWQILFFSPDHFNNDFY"
     gene            13199..14386
                     /locus_tag="LGJ20_003877"
     CDS             13199..14386
                     /locus_tag="LGJ20_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HBK2387883.1"
                     /translation="MSPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNPFKLDSTNFTNANMFQVNFVSSTQNVTISWGYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNYLNRIKLPEMKGFSSEKIDYYIDIIIKDYESVKENAYLIDPKINYNTD
                     LNIEDSSSEEFLSDNTLEKDENSPDNCFEVVKYNTYEAYNSENQYFTREDYTYNYDLL
                     NAI"
BASE COUNT         4133 a         3751 c         3252 g         3709 t
ORIGIN      
        1 aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca caacaacatc aacaagttga
       61 gcaccccgta ggccggataa ggtgcgccag catcgcatcc ggcacgatac ccaaaacctg
      121 gcgtttatct ggccttgctc gccataatct cgataatctg ccgatccgtt tgctgcatcg
      181 aatggcttgc taacgcacac aggttggcaa tcgactgctc aacatcatgc gccacgatcc
      241 cttcattgcc ggtcacggcg gtatcatcca gcgccattaa caccgctttc cacgcagccg
      301 aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc accatcgcaa atcatgccgc
      361 tgacatcgcc gatcatactg ctgatcgcca tcgagatggt ttcataacgc ccatccacca
      421 gccatgccat cccggcggcg gcccccattg ctgcggtcgt tgcggcacac agcgcagaca
      481 aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg agaaagcatc agcgcacgcg
      541 ccagccgttc atcatccgct ccgaagtgtt ctgctaccac caccacaggc attgttgcgg
      601 tgatcccctg gttacccgag ccggagttac tcatagccgg aagcgtagcg ccgcccatac
      661 gcgcatcgga tgccgcgctg gtacgaatca caatggatga agagagatct ttcgccagca
      721 aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg cagcccccac ttaccgctca
      781 aaccttcctg cgataacgca caatttagct tcgcggaatc gagaataaag cggatcgccg
      841 caaacgggac ttcattgacg aacttcagga tctcagccag cgtcgttctg gaaagcaccg
      901 tcagcggaga ctcttgctcg ccctctgcca cacacgcctg ctgggtaaac accacaccat
      961 tgtgcgtctc aatatgcaca atgttggtat gcccgccgac gatggtgaca cacgcccact
     1021 tctcaccgtt ccagactttg gcgcgtgaga agaggatttc attgcaaggt tcctggatct
     1081 taacggagac tttccccgcc gccagcagtg ctttggcatc ggcaattgcc tgcgcagttg
     1141 cgtctttcag cacttccagc ccggcgttgg catttccacc taacgccccc agcgccgccg
     1201 caatcggcag ccccaccatt cccgtgccgg gaacggtgac gcccagaccg ttcttcatca
     1261 gatttggcga aacccaggct tctacacgtt caaccggacc ttccagttct gccgcagcaa
     1321 ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc cagcgccggt tttacttcct
     1381 cctgaacggc gaggatgtaa cgctgccata acggatttaa agtcgaatca aacatattaa
     1441 aaaccttaaa atttcaggta aatcaagaaa atgccaggaa cggagaaaca caaagcaaca
     1501 aaccagtgac gataatcagg tacagagaca tccctttgta tttgtgcaat gccggtactt
     1561 tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc gaaaatcggg ctacagatag
     1621 aggtgaagct caacaccggt gcgttcagta cgatggcgct ccaggccagc aaaatggcga
     1681 aaatcatgat gccgcgctga acaagatttt cgttaatctt ctcggcaggc atcttgcgac
     1741 gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa gcctaaatag acgccaaaga
     1801 atgcagtcat gacagcaaag atattgagaa tgacgctgac cactttcacc cacgctgcgc
     1861 cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga aatattctgc tcataggctt
     1921 ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac ggcgtagaaa aagacggtga
     1981 caaacaaaat gccaaacgcg atattcattg cccgcaatgc tttatgccgc gccacttcaa
     2041 ttgatttttc ccgcgagcga taagagatca ccatcgggct taacgtctgg ataaacagaa
     2101 tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt caccagcagc cccagcggcg
     2161 gtagcgaacc gacgttatac agatgccaca ttcccaccat cgacacgccc agcgccgcga
     2221 ccaccagcag cttggtcagc accatgccgg tcgaaatttt gaataacaat ttctcgccgc
     2281 gtgaagagat cgccaccaga atgcaaatca gtaccagacc ataaaaggga ctgtctgaca
     2341 gcaacccttc cgtcacgccg aaggtatgca gataggaagc actatcgttg gtgatggcgg
     2401 tggaatagac gaacatccaa atcaccagca ttacgaaata gagcgcacct aacaggatgc
     2461 cccagttttt acctaaataa ccgctaatga cactcgggta atctttacat tctggtgatt
     2521 ctgccagcgt attaataaac aaccgctgaa acagatacat tgccgggtaa ccaatcaccg
     2581 atgagagcaa aaatacccac aatcccatca aaccgacctg caccgggaga aaaacaatcc
     2641 ccgcgccaat cgccatcccg atactcataa tcacccagcc ggtgtcagta ctgtcgaatt
     2701 tgatcgcttc tcgccactcg ctctcactca ttccagcacg acccgccggg gtcgaagcgt
     2761 ctgcaatgac gcctttattc gatgcaattt ccataatttc tcgctcaata ttttgtaggg
     2821 cttattattt ttttccgagc cgcatcaagg cgatatgcgg tctacgtttt tgcaggcgag
     2881 ttattagaat aaagaaatga tacgcgcgag gtcagagaaa atcttcacaa tcaagacttt
     2941 gaaataacgg agatggataa gaattttcta ctaaattaat cgcaagagaa tattcattct
     3001 ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg cacccaagga tgaaagctga
     3061 cagcaatgtc agccgcagac cactttaatg gccagtcctc cgcgtgatgt ttcgcggtat
     3121 ttatcgttca tatctttgcc ggtttcatac atcgtctcga tcactttatc gagtgaaaca
     3181 cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt ttactgcttt cacggcatta
     3241 atggcattac gttcaatgca cgggatttgt acctgtccgg caaccggatc gcaggtcagc
     3301 ccaaggttat gctccatcgc gatttccgcc gcattgcata cctgcgccgg actgccgccc
     3361 agtagttcag ttaaccctgc cgccgccatt gaacaggcca cgccaatctc cccctgacag
     3421 ccgacctccg cgccagagat ggaggcgttc attttataca gcgcgccaat agcccccgct
     3481 gccagaaaat agcgggcaat tgaccgctcg tttaccggac gacggaactt atcgtaataa
     3541 gccagcactg ccggaataat gccgcacgca ccgttagtcg gtgccgttac cacgcgcccg
     3601 ccagctgcgt tttcttcact aaccgccagc gcgtacatgt tgatccagtc gatgacattc
     3661 atcggatcgt tagagatgtt atcgctagaa accagctgac gacgcagcgc tacggcacga
     3721 cgcggcacat tgagcggacc aggcagcacg ccttcagtgt tcatcccgcg ttcaataccg
     3781 tcatgcatca cttgccagat acgggcaaac cctgcgtcaa tttccgcttt actgcgcagc
     3841 gctagctcgt tgtgcatcat caggccagat atagacaggc cgttgtaatc acacattttc
     3901 agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg tttcgacatc gtgcgacagg
     3961 ccgaagtgtt cttcttcgac aataaacccg ccgccgacag agtaataggt tttacttaat
     4021 agcgcttcct gtcccttcca ggcagtgatc cgcattccgt tctcatggcg aggcaacatt
     4081 tcgggatgga agataatgtt ctttgctaca ggaaaatcaa caatatgcgc accagatgcc
     4141 actggcagcc gcccgctgcg cgttactaac tctataaatg cagggatctc atcaatgaca
     4201 acatcctgcg gactgtttcc tgccagtccc atgataatgg cgacatccgt ggcatggcct
     4261 ttgcccgtca gtgacaacga cccatacaga tcgaccacaa tatggctcgt cgcggttaat
     4321 aagccgctac tttccagccg atcaataaaa ctttttccgg cattcattgg ccccacggta
     4381 tgcgaactgg agggaccaat cccaattttg aaaatatcga atgcactaat catatccaca
     4441 ccctcggatt gccgttcagt gaagtggagc ggaacgacct tgcgaccgtc ccgctcacga
     4501 ggctttacgc actacgtact gcgatagctt caatttccag cttcacatct ttcggcaaac
     4561 gcgcaacctg cacacagctc cgggtcgggt aggtcgcctg atgctcatcg aagaactgct
     4621 tatagacttc attgatggtg gcaaaatcat tcagatcggt gatgaacacg gtcatcttga
     4681 tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc tttgacgttt tcgaggctta
     4741 aacgcgcctg atcttgcaca tcagccggga tctcaccggt ctgcgggcaa accggaattt
     4801 gcccggaggt gaagaccatg ctgcctaaat caacgccctg aacataaggg ccgattgcgc
     4861 ctggggcacg ttgcgtttcg ataatctttt tcatacatcc tccggcgtca gagcgcctgg
     4921 gtaaaagtac gtgaaataac atcctgttgc tgttcacggg tcagtgcgtt gaagcgcacg
     4981 gcgtagccag agacacggat tgtcaggtta ggatattttt ccgggtgctc gatggcatcc
     5041 agcagcattt cccgattcat tacgttgacg ttgaggtgtt gaccgccttc gacatccgct
     5101 tcgtggtgga aatacccatc cagcaggccg acaaggttgg ttttacgtac tggatcttct
     5161 ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga tcccatcttt ggcgtaggtg
     5221 aacggcagtt tcgccaccga cgtcaatgag gccacggcac ctttgcggtc acgaccatgc
     5281 atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac gaccgtccgg cgtattaccg
     5341 gttttctggc cgtacaccac gttagaagtg atagtcagaa tcgactgggt agggacggcg
     5401 ttgcgatagg ttggcagcgc tttaattttc ttcataaagc gttcaaccag gtcgcaggca
     5461 atgctgtcta cgcgctcgtc gttgttgccg tactgcggat attcaccgtc gatttcaaag
     5521 tccaccgcca ggccgttttc gtcacggatt ggtttcacgc gggcatattt gatggcagac
     5581 agggagtccg tcgccaccga caggcccgcg atgccgcatg ccatagtgcg atagacatca
     5641 cgatcgtgca gcgccatcag cgaagcttcg tagctgtact tgtcgtgcat gtagtgaatg
     5701 atattcagcg cgctgatgta ctgcaccgcc agccagtcca tgaagtgatc gaggctgtcc
     5761 atcactttgt cgtaatccag cacgtcgtcc atcagcggtg ctgttttcgg cccgacctga
     5821 atcttcagct tctcgtccac cccgccgtta attgcgtaga gcagcgtttt cgccaggtta
     5881 gcgcgtgcac caaagaactg catttgctta ccaatcacca ttgggctgac gcagcaggca
     5941 atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat cgtcattttc atactgcaag
     6001 gaagaggtga cgatcgacac ctgcgcggca tattttttga aggcaatcgg taattcttcc
     6061 gaccaaagaa tggtcaggtt aggttccggt gccggcccca tagtgtgcag ggtgtgcaaa
     6121 tagcggaagg agtttttggt caccagcgta cgaccgtcca gccccatccc gccgatcact
     6181 tccgtcgccc agattgggtc gccggagaac agcgaatcaa attccggtgt acgcaggaag
     6241 cgtaccatac ggatcttcat gatgaagtga tcgatcagtt cctgtgcctg ctgctcattg
     6301 agtacgccag ctttaaagtc gcgctcaatg tagatatcga ggaacgatgc cgtgcggccc
     6361 agcgacatcg cgccgccatt ttgcgatttc actgccgcca gataagcgaa gtagagccac
     6421 tgcaccgctt cctgcgcatt ctgcgccggg cgagagatat caaagccata tttcgccgcc
     6481 atttcctgaa tctgcaacag cgcatgacga tgctctgcca gctcctcacg cagacggatg
     6541 gtggcttcca gatcctcgcc tttttccaga cgagactgga gatcggcaaa ttgcagttcg
     6601 cgttcacgta ccagataact gatgccatac agcgctacgc ggcgatagtc accgataatg
     6661 cgcccacggc catagccatc tggtaaaccg gtcagcacgc cagatttacg gcagcgcagc
     6721 atatccggtg agtaaacatc aaatacgccc tggttatggg ttttacgcag atcggtaaac
     6781 agatattcaa attcactgtc catttctcgg ccataggcgt ggaatgaact tttaatcata
     6841 ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg catccgtttg caggccaaca
     6901 attttttcca gcggctggtt aatatatccc gcatcatgag cggtaattgt ggtggcaata
     6961 ttggtatcga aatcaaccgg cgcgtgggtt gcattttcga tacggatgcc ttccattact
     7021 ttttcccaca attccgtggt ggcaggcgtc gcttcggcga ggaaagattc atcgccttca
     7081 tacggtgtat agttatgttg aataaaatcg cggacattaa tttcgttttt ccagtccgta
     7141 cctttaaagc caagccatgc gtcggcgtac agcttatcgc tggtatcaat atctaccttc
     7201 atgaaaaata atctctctac aatacttcaa ctaaattatg caaattctgc gggcgcgtta
     7261 actttgccta aatgaatggc atccaaagca atcatttttt cttcgttagt cggaataacg
     7321 gcacaaatga cacgcgcatt ttcactggaa acaattcgct caccacagga gttagagcga
     7381 ttattcattt ctgtatcaat ctctacgcct aatacagcca aatgttccat gaccagacga
     7441 cgaattaagc ttgaattctc tcctattccg ccggtgaata taattccatc caggcgacgt
     7501 aatgaagctg cgtgtccggc aatatgacgg gcaattcggt gaacaaaggt tttaattgcc
     7561 agttgcgcgc gttcgtgacc ttcatgccag gctttttcca gaacacgtaa atccgaagaa
     7621 agaccggaaa tacctaataa tcccgactct ttattcacta cgcgttccag gtcacccagg
     7681 ctctggttgg tttggctggc gacccaggac atcgcaccaa agtcgacatc gccactgcgg
     7741 gtacccatca tcaagccttc cagcggcgtc attcccattg aagtatcaac actctgaccg
     7801 ttgcgaaccg cgcagattga cgcgccattg ccaagatgcg ccacaaccag gccggagtca
     7861 tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa catagcggtg cgacgtgccg
     7921 tggaaaccat aacggcgtac acctaactct tcataatatt tccacggcag gccgtataaa
     7981 taagcttccg gagccatcgt ctggtggaaa ctggtatcaa ataccgccac ctgagttacg
     8041 cccggaaata attgctgcgc cgattcaata ccacttaaat tggcgtaatt atgcaggggt
     8101 gccagtggag aaacgcgacg gatattatca atgacttcat cggtaataat ggcggactcg
     8161 gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta aggccacact gtcatttaaa
     8221 ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac cttcgtagct gtggtgagcc
     8281 agcggtgctg gctctccccc atttacggat aagaatgcat tttccgagtt aataccgtcg
     8341 gcaatacctg acattaatac ttcacagtcg ctggcatcga gtacggaaaa cttaatcgaa
     8401 gacgaaccac agttaataac caaaacaacc ggaaattcat tcatctcttt tctcatcctg
     8461 agttacggat taaaacagtt tgtatacgat gttcaggatg gtcagcagac caatcacggt
     8521 aacaaacacg ttatccagac gaccacggta tttcgccaga gacggcgctt tacggatggc
     8581 atacatcggc aacaggcaca gcagggatgc gataatcggt gcgcccatgg cttcaataag
     8641 gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg gagcccatga tgaagatcat
     8701 gctgatagtg ttcagtttac ccagcgacac tttagttttg tcgcctttat aaccgaactt
     8761 cagaatcaga ccattcaagc cttccagcgt ccccagatag tgaccgaaga aagatttgaa
     8821 gatagccacg agtgcgatga tggaagccgc atattccagt gtaatcgcga acgttgtttt
     8881 ggtaccggtc atggacgcaa agtggttagc cagataagaa agcactggaa tattctgcgc
     8941 tttggcttcc gccatgttgg ccggagacag cgtaaacagg cagctaaagg caaagaacat
     9001 caccactgca accatcagca tgctggcacg agaaatgatt tgggaacatt tacgttcggt
     9061 gaagtcgcga ccgaagtctt tctcatactc ttcacgttta gaaaccacga aggaagagac
     9121 gattggcgag aagttaaagg agaaaaccat gatggaaatc cccagccaga cagtgatcag
     9181 gataccgtca tgaccggtta acgacagcga accgaggtca acctggtcga taactgcaga
     9241 gttccagtaa gggatcagcg acagagaaat cagcaccagg ctggcgataa acggccatac
     9301 caggtagctc atcactttaa ccatcagatc cttaccaaac cagatgacga aagccatcag
     9361 cagcaacagg aacagcgcca caaagccgcg attcagcggt gcaaatccga gctggttttc
     9421 ccagaacgtc ataaaggtat tggtaatagt aacgccataa atccacagca gtgggcaaat
     9481 cgcgaagaag tacaggaacg tgataaccac gccgccagtt ttaccaaaat gctcttccac
     9541 cgtttccgta atgttgccgg aagggttaga gccagaaaga cacagacgcg ccagcgcccg
     9601 gtggcaataa aatgcgatgg ggtatgccaa taccaacatc agaagaatcg ggatcagtcc
     9661 gccaaaacct gcgcggatag ggaagaacag caccccggcg ccgattgccg taccaaacaa
     9721 gcctaacgtc catgtggtat ctgatttacg ccaggacgat tgttttgtct ggctggatac
     9781 aatgctatct gaagtactca tatcctatcc tcaacgaatt aattaagcgt caacgaaacc
     9841 ggtgatttga gagacgcgag aaagatcgat attgccgccg gaaataatac tgacggtttt
     9901 tctgttttga atatattggt ctaatttacc gcttaataat gcagcacatg ccagagcgcc
     9961 tgcgccttcg gtgacgactt tattgcgctg aattaaggca atcatactgt ttctgatttc
    10021 gtcttcgctg accagcacga tgtcatcgac taattcacga acgatttcgt aagttaaatt
    10081 acccgggcgg gagacatcac aaccatccgc cagggtgccg gtagttcggt gcgtggttat
    10141 ttctccggag tggaaagaag ccgccatgcc gtgaacgttt tctgactgta cgccaataac
    10201 acgaatggtc gggttaatag atttaattgc caccgcaata ccagcaatta aaccaccacc
    10261 accaattggc acaatcacgt tatcgacatc atagagatct tccataattt ccagaccaat
    10321 cgttccctgg ccagcaatca ctttcggatc atcgtaaggt gggataaaaa tacgaccttc
    10381 catttcgaca atttcgctca ctttagcgat agtgtcgttg aaattatcac catgcagaac
    10441 gacttctgcg gagtagtcgc acgttgccgc tactttggat tttggcgcac cttttggcat
    10501 caccacttta ccgtcgatac ccagcatcgc gcaggagagc gaaacccctt gcgcatggtt
    10561 gcccgcagaa caggccacta cacctttacg tttttccgca tcagtcagtg aacttaattt
    10621 attaaatgcg ccacgaattt taaatgaacc cgtacgctgc atattttcaa acttcaggaa
    10681 tatttcacct ttgcaacgtt cactaaaata gtttgagcga ggcatgcctg ttttataaat
    10741 tcgcccagcc agtcgttgtt tcgcttcaat aatgtcatca atagcaaccg gcagatcgta
    10801 tgtaatatgc attataaaac ctcttcgcct gaattaaata tgtaggtaac cgtaaccgac
    10861 acctgcaaaa cagacaggtg gattatttat gtttaaataa aaacaaataa ctaaccaact
    10921 tctattaatt gccttcgtct acacccatta taagatgaat actctttggc taattccacc
    10981 aaaaccgatg ctgctttttt aatacgataa tttttcgacc ataccgcggc atattgtgcc
    11041 acaggtaatg tttcttcaac cggaatagta ataaattgat ttgaaccaaa aggtgacgtc
    11101 atatcacaag gaattacagt taagaaatca gcattgagaa caagattata aattgtcacg
    11161 actgagtcgg ttttaacgat gttttcaata ctgatgccat ttctttgtaa cgtagtaaga
    11221 agctcgctgt agtaccccat attggtttgt ggcaacaccc actgttcgtt cttcaacgac
    11281 tccagcgtgg tggtgccggt gcatgttcgg gacttactgg ctaccagcac aaactcggac
    11341 tcgaacagcg gctcaacatg taaatcctga agcttcattt ctgcacttaa cgtaccaatc
    11401 gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg aagacagttg cgcttcatac
    11461 atagaaacct gcgctttcgg gaacacctct ttgaacttgt tgatcatccc tgacataaaa
    11521 gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca ccaccgcctc agaagacata
    11581 ccgcttatct cattaaccat atttttcatt tcacgggtaa tggattcgga acgggagagt
    11641 aacaattgac cggcaggtgt taatgttaca ccggtatttt tccgcaccac taattccaca
    11701 ccaaaataat cttcaatatc gttaatgatt ttactgacgg ctggttgagt taaccctaat
    11761 tcttttgcag ccgagccgat agagccactt ctaatgactt cctgaaagac taccaggtgc
    11821 tgcgttttcg gaagaagaat agtgctcata acgacctacg ttaattacct cattgacggc
    11881 atgaagtgta tcaaaatgaa atgaacagga tatgtgcgac cactcacaaa ttaactttca
    11941 atactttcca tggtatcgtt acgcaaatta aataccttta aatatcaaca agttaaagta
    12001 taaaaatcag cataaaccct gatttttgtc aaaaaaaatg acggggataa ccatatttta
    12061 tggcgataac atcattcgtt atgtggttaa tataaaaaag gggctgagac catatttcaa
    12121 gcaattaccg gataattacc aggcaaaatt tgagttaaat ttaatgagca agttctcaaa
    12181 ttttattata aataaaccat tttcagcgat aaatactgcg gcacgtcaca ttttttcacg
    12241 ctatttgttg gagaacaaac atttatttta tcaatatttt aaaatttcga atacaggtat
    12301 tgatcatctc gaacaattga ttaacgtcaa ctttttctct tctgacagga cgtcattttg
    12361 tgaatgcaat cgttttccat aaattcttct cccctcatag gcgacgaata gcattttgtg
    12421 ttgaggatca caaaacgaat aattgctggt cgccgcgata aggtcagacg aagtcaacaa
    12481 gggggatttt cactgtgctt tacatcagcg taggacacag aatgttgctt cgtttactgt
    12541 aacgccgggt aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg aaaataagca
    12601 tctattattg tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc caattgcaca
    12661 tatttttcac ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca atagaaatat
    12721 tgattattgg acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg acgttaataa
    12781 aatttacagt ataagtaatg aactgcggag agtattatct gcaattactg cattgaattt
    12841 ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa ccggaaaata tgagtccatt
    12901 catcatagat atttctacag gagaacatga tgattatatc atacaaacat tagatgtagg
    12961 cacttttgca ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa aagagctgga
    13021 atgtattaag gagacgattt ctaagtattg cgcaaagttc acccaaaaag aagccatctt
    13081 aacaccccct acgcacttta ataaaacctg cattacttca gattattggc aaattctctt
    13141 tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg ataaaataat
    13201 gtcccctgtt tcatcaattg gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa
    13261 tgaccttcct gaaagcccca taggaaataa cctcgaagca ctagcaccag gtatagaaaa
    13321 actaaaacag acctctattc aaatggtcac tttacttaac gcgttacaac caggtggaaa
    13381 atgcattatc actggtgatt ttcaaaaaga attagcgtac ttacaaaatg taattcttta
    13441 taatgactcg tctcttcgtc tggatttttt aggttataac gcccaaatta ttcaacgttc
    13501 ggacaatacc tgcgaactta ccattaatga accgttaaaa aaccaggaaa tatctacagg
    13561 caatatcaat gttaattgcc cattaaaaga tatttacaat gaaatcagga ggttgaacgt
    13621 aatctttagt tgtgggactg gagatatagt tgatctatcc tctctggact tacgtaatgt
    13681 cgatttagat tattatgatt tcacagataa acatatggct aatactattt taaatccttt
    13741 taaattggat tcaacaaatt ttactaatgc caacatgttt caggttaatt ttgttagttc
    13801 aacacaaaac gtcacaatct cctggggtta tttactaaaa ataacgcctg ttttaataag
    13861 cattagcgat atgtattctg aagaaaaaat caagtttgtc gaaagttgtt taaatgagct
    13921 tggagacatt accgaagaac aattaaagat tatgagattt gcaattataa agtctatacc
    13981 aagggcaact cttacagata aattagaaaa tgaattaaca aaagaaatat ataaaagctc
    14041 atcgaaaatt aacaattact tgaacagaat taaattacca gagatgaaag gattctcatc
    14101 agaaaaaata gattattaca tcgatataat cattaaagat tatgaaagcg tcaaagaaaa
    14161 tgcttatctt atcgatccaa aaattaatta caatacagat ttaaacatag aggattctag
    14221 ctcagaagaa ttcctttcag ataatacact cgagaaagac gaaaattctc ctgacaattg
    14281 ctttgaggtt gtaaaatata acacatatga agcatataac tcagagaatc aatattttac
    14341 cagagaggac tatacgtata attacgacct tttaaatgca atatagactt aataacttct
    14401 tacaaagtct attattaata ccgtagatat ttattcatct taccctgtgc accaaaagca
    14461 aaaactcact gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg atgcgctacg
    14521 cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg taggcaggat
    14581 aaggcgttca cgccgcatcc ggcatgaata aagcgcactc aacaatctga aacccgccgg
    14641 aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt ttgggtatag
    14701 tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc cgaagcgggt
    14761 ttttacgtaa atcaggtgaa actgaccgat aagccgcttt cttttgggta tagcgtcgtg
    14821 gacagtcatt catctttctg cccct
//
LOCUS       DAFATA010000106        14479 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFATA010000106 DAFATA010000000
VERSION     DAFATA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.107"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..237
                     /locus_tag="LGJ20_003878"
     CDS             <1..237
                     /locus_tag="LGJ20_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="tyrosine specific permease; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBK2387884.1"
                     /translation="ALFYPRGFVMALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYR
                     VKGGRPALVVVFLCGIAVIGVQFLIAAGLLPEVG"
     gene            complement(299..964)
                     /gene="yecA"
                     /locus_tag="LGJ20_003879"
     CDS             complement(299..964)
                     /gene="yecA"
                     /locus_tag="LGJ20_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBK2387885.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(1321..2322)
                     /locus_tag="LGJ20_003880"
     CDS             complement(1321..2322)
                     /locus_tag="LGJ20_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK2387886.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(2328..2675)
                     /locus_tag="LGJ20_003881"
     CDS             complement(2328..2675)
                     /locus_tag="LGJ20_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HBK2387887.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(2705..3355)
                     /locus_tag="LGJ20_003882"
     CDS             complement(2705..3355)
                     /locus_tag="LGJ20_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387888.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(3371..3775)
                     /locus_tag="LGJ20_003883"
     CDS             complement(3371..3775)
                     /locus_tag="LGJ20_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2387889.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(3865..4002)
                     /locus_tag="LGJ20_003884"
     CDS             complement(3865..4002)
                     /locus_tag="LGJ20_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387890.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            4074..4277
                     /locus_tag="LGJ20_003885"
     CDS             4074..4277
                     /locus_tag="LGJ20_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK2387891.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            4299..4649
                     /locus_tag="LGJ20_003886"
     CDS             4299..4649
                     /locus_tag="LGJ20_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HBK2387892.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADF"
     gene            4660..4938
                     /locus_tag="LGJ20_003887"
     CDS             4660..4938
                     /locus_tag="LGJ20_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387893.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            4950..5192
                     /locus_tag="LGJ20_003888"
     CDS             4950..5192
                     /locus_tag="LGJ20_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HBK2387894.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAEHAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            5189..5302
                     /locus_tag="LGJ20_003889"
     CDS             5189..5302
                     /locus_tag="LGJ20_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387895.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDE"
     gene            5395..5811
                     /locus_tag="LGJ20_003890"
     CDS             5395..5811
                     /locus_tag="LGJ20_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387896.1"
                     /translation="MNGKEEGIIDTLKKISEAEDEMAKDAVKRSQHMAALHALTIAKI
                     TADAAKIIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            5835..6038
                     /locus_tag="LGJ20_003891"
     CDS             5835..6038
                     /locus_tag="LGJ20_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387897.1"
                     /translation="MAIKHFSVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            6035..6301
                     /locus_tag="LGJ20_003892"
     CDS             6035..6301
                     /locus_tag="LGJ20_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK2387898.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTNMFNQLLKEARA"
     gene            6298..6597
                     /locus_tag="LGJ20_003893"
     CDS             6298..6597
                     /locus_tag="LGJ20_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387899.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            6609..7226
                     /locus_tag="LGJ20_003894"
     CDS             6609..7226
                     /locus_tag="LGJ20_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBK2387900.1"
                     /translation="MHTQKNRLPCRNRSGYISAAPHKTGAGILNPIQSKAHNRASGFF
                     VRTVLPRLFRVRIMAGRTGPTSVGPDSLLSGVENPVRLATPRFSTLDGELFLSPSKEA
                     TPWQTANSTALSRSVVTSRLKSTADFPAHHASRKSCTSICCMSAATHYQTFIPPLFSA
                     IWRMICTSFNSSSSSKTNSINSCSGPFLHLAAGGLRTSVVKRIAA"
     gene            7223..7612
                     /locus_tag="LGJ20_003895"
     CDS             7223..7612
                     /locus_tag="LGJ20_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HBK2387901.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQEWQRVTGYQP"
     gene            7609..10449
                     /locus_tag="LGJ20_003896"
     CDS             7609..10449
                     /locus_tag="LGJ20_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBK2387902.1"
                     /translation="MSAFAWSWNEPRPAIDPARFTERRQETETDLQRAIRYYLEADKR
                     AQEEQEAKEEAFFAQSAMGKKLMASLEEAGQREKLAQSIISKRRATEQDPVARAFATL
                     KALPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYTRGTLRKIFERLDRTDGRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARIALHLHIMPPHYDALTTDKDRRNEPDTELLPGAILRLTCTEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPTPRPQLPDGVDVNEWMRSLKRHERRALMHS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            10526..11485
                     /locus_tag="LGJ20_003897"
     CDS             10526..11485
                     /locus_tag="LGJ20_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBK2387903.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            11490..11801
                     /locus_tag="LGJ20_003898"
     CDS             11490..11801
                     /locus_tag="LGJ20_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBK2387904.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            11865..12455
                     /locus_tag="LGJ20_003899"
     CDS             11865..12455
                     /locus_tag="LGJ20_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HBK2387905.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(12946..13992)
                     /locus_tag="LGJ20_003900"
     CDS             complement(12946..13992)
                     /locus_tag="LGJ20_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2387906.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDIQPTS"
     gene            complement(13992..>14479)
                     /locus_tag="LGJ20_003901"
     CDS             complement(13992..>14479)
                     /locus_tag="LGJ20_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387907.1"
                     /translation="VVVPPSVPGGRFRTVETRRVQGLEFEEQARVIEEFTCRYNVEHI
                     GIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQIMRAGRWEYDRAEREL
                     VAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVINEPIGGEGENERFTVME
                     F"
BASE COUNT         3891 a         3881 c         3428 g         3279 t
ORIGIN      
        1 gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt ggcgctggcg
       61 gtactggcat tgattatccc ttcgctgttg acctggcaaa gcagaaagca caatcctcag
      121 gcgggttacc gggtgaaagg tggtcgtccg gcgctggtgg tggtgtttct ctgtggtatt
      181 gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt taccagaagt ggggtgatca
      241 gatagcctca aattccttat tgggtgccag aattaacgct gacacccaat ttgacctctt
      301 aatgcaggca gcactgctta aatttcttac cactaccgca cgggcaagga tcgttacgcc
      361 ccggtttctc ttctgctttg atcggttgct gaacagcttt ttcctgcgga tgcgccatcc
      421 agtacgcatg tagatcaagc gccgccagtc gaatggcatc tacgctctct tcaaacgctt
      481 ctggcgacat tttctctacc cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct
      541 ccagcgctgg ttttaacgaa tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca
      601 tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcaact tctcgcaagc
      661 cgaataacgg ctcaaactgc tccgggaatt cgttaagacg ctctgcggta tcggccatat
      721 gttgaaaagc cagattcata aagcgcgtca tctctttctc tgacgcccag cgcggcacat
      781 agtcagcccc accccacacg gcaaccagcc actgttccgg ttcaatctct tgcggagaac
      841 tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aaggatggcg tggtcagtgt
      901 tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac ggtcccgttt
      961 tcatacgctt ttccttgtgg atctcaactc gccagcacct atcttacatg ccggtccgta
     1021 tcagagatac tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca
     1081 agttgcaggc acaaaaaaac cacccgaagg tggtttcacg acactgctta ttgctttgat
     1141 tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc
     1201 cgagggattt taaaaactat caaccaccat ttataaatca taaccttatg attttattaa
     1261 ctttgaaaat ggctctatac aacgccattt gaatctattg tcacttttta tcgccacttt
     1321 ttaccctcta ttgtcaaaag ggttcaattc aacagcagcc tctaaatgac ctggagcaaa
     1381 atgcgcataa cgcatagtca tttttatatc gctatgcccc agtatttttt gcaacacaag
     1441 aatatttccg ccccgcatca taaaatgact ggcaaacgtg tgacgtagca catgagttaa
     1501 ttgcccatca ggaagctcga tcttcgctct cttaattgca gcgtcaaaag cctcataaca
     1561 tggtgaaaat agcgctcctc gttttttagg aagcatagct tgcaattgag gtgaaatcgg
     1621 tacagtgcgg ttcttctttc ctttagtttt aacaaatgtg attcgaccgg gcagtacttg
     1681 agattgcttt aatccttctg cttcactcca ccgagcacca gtcgcaagcc caatacggac
     1741 aacaaccccc aaatctttat tccgtgactc atcacacgca atcagaaggc gttcaatctc
     1801 atctacatac agaaacgcca gttccttttc ttcctcacga aacttgcgaa taccagtcag
     1861 ggggttttca ccagaccact ctccaaggcg cttcagttcg gcaaaaacag catgtagata
     1921 tgactgctcg cgattaacgg ttgcttcact aagttttttc ttccccttgg gattccattc
     1981 tcctgatagc cttctttccc gataagtagc aaacatattt ttgtcaaact gagaagcaaa
     2041 tggatctccc agcctggaac aaatcgcctc aagtttgact ttgcgctctg caccagagga
     2101 caaggtttta ccgtacatct caaaccaacg agcaatcaac tcagaaagac gaggaccaga
     2161 accatcttga aactcgtctc caactctact attcattaaa cggcgctcat aagagagcgc
     2221 ctcacttttt gtcgcaaact gtttacgaat gcgttttccc gatgccccgt agggataaca
     2281 ttcgcaaagc catttacctg atggaatctt acgaaccgac attttagtta cttatcacat
     2341 aaatcaaatg cagccttagt gacatccccc agactctttt ttaaccctgg ggcggcatca
     2401 ttatctagcc aaaatggatt attgttatct aacggtaacg caccaaatgt tttacctttt
     2461 attcgagcca aacctgtaag tgcatataac ttattatcgt caaaattcat cacataagga
     2521 ttaccatcaa gacactgtaa ttgaacctca tcagtattaa atggccatac cccattgaaa
     2581 ctctcacgtt caatagtttt aaaaggcatt gcgacggcgg aaaaagaaaa catagataaa
     2641 aaagtaacta atagttgaac cttttttact ttcatatcat tacctcaatt tagctcaagt
     2701 aagattacaa attaaaaaac gccctagaaa tgacaccgcc taccaaaacc cctacgcaga
     2761 taaagaatat tatttctttt ggataaagtc ggattaattc tgaagcacga agtcggactt
     2821 ctggtaaggt cgaactctct gtgtggcttg atgccgattg ttgctctaac cacgacaatg
     2881 cagactgtaa ctgagaacga gtaagatcgt ttaaacgtcc tgtaccgaaa ttgatatggc
     2941 aataccgcag aagtttttgt cgaagtccac agtcttcact gttacgtagt aataaactta
     3001 caagagcctt acaggcatca tgatctttac atcgctcaag cattgcatgc agaaaactct
     3061 ccgctgtttt atattgattt actgtcatat catcaatacc agctacacca atctccgcat
     3121 gtactttttg ccaaataata aacgcttcag tattgctagc ttctgcaata gcagcaacca
     3181 agctatttag ctccttacgc tgagccttaa gcaaagggcg atcgtcatca tcattattcg
     3241 aagggattgc gatattgacg gtatgagaac catcatatcg ctctatctga atattctttt
     3301 cgtgaaaatc acgcccagca acgcgattgt ttgaaccgtt tgagttgacg gccatgtcac
     3361 ctccctacta tcacctaccc ttagtttcgt tatagtcacg accagcgata cggttattac
     3421 caccagaaat atttaactca cgtcctgatg gctgagtttc cttttcactg atcgcacctt
     3481 ttaaagcccc aatcaccgcg tttttcacat ctaacgaagc tgctcgaaag cgagtaatca
     3541 attcctgctc atcatcgtta taagtttcag gtgagtgaat tcccaacaca acatactgaa
     3601 catcaaggcc aaaacgagac agcgctgcca aatacgcagc atcaggaaag ctatctcctt
     3661 tctcatatct aagctgagtt agctttttga ctccaccaat gtcgctcatg gcaacttgac
     3721 taagtcccaa tctttccctt tcctcacgca accgctgacc aatatcattt ttcatacaaa
     3781 aaccttgaca ggtatctttt ttgataccaa aatgatttca cgagctatta gatgatcaca
     3841 atataccact atgaaacaag ttcttcacga taccagatca cgcattccgc gtaacaccgc
     3901 cacaggtcca agactggcac ttcggctgtc ccttgaggag cgagccgtca ttgatgaaat
     3961 ggcagctaaa gaacaacgct catcctctaa catggcgcgc atgatcttcc ttcgcggcct
     4021 agagctaacc cagaaagaac aaaacaaatc ttcctgatca ggaggctagt gggatgtcag
     4081 gtataaccat caatatcaat gtgaatgccc cctatgtatc cctgcagaaa tatgcagaga
     4141 taacaggtat ccctcttaat acatgcaaaa agatgttggc tgacggtcga attattatcc
     4201 gccccaaacg cgccaaaatg gaaaagcctg aagtaaacct tgtggcgatg ttaaaagacg
     4261 ctttggctaa cagctaaaac aatgaacaga gcaccatcat gaaaaaaaac gctaataatc
     4321 catactccaa atttcgtaat ggcgtagaac gccatgtaca ccacgtcgct accagtgcat
     4381 cacgtagtaa cagtcgctat aacctgaacg agacgcacgc aacaccggat ggccacgctg
     4441 taaaacaaat cggcgaacat gcctggctga ttgagaaagc tggaatcgtg gtccacaaat
     4501 gcccacgcaa tccgtttacc ggaaaccgca tttttgcatt gaactgcggc gacaatcact
     4561 tcgggcagga tttcacatta tacgaagcac ttcgcacggt tgatcgtctg cttcgcgggc
     4621 aaagttttat taaacaggct gatttttaac aggtgcttta tgaccaaaga gcatgcacaa
     4681 ggtgtattta tccgttttat tgattttcgc ggtgaactgt tattacgcgc atcagccatt
     4741 gacggagttg ttccatccga aaaaaacgca gctacttacg tttatctgaa cggtacgcgc
     4801 ctgactgtgg agcttccgta ccagaccgta cgagaaatca ttagtgaagc tgaaaaggca
     4861 cgtcaggtta atggcgatga accctatatc gaaattattt gtatggattc agaagctgaa
     4921 attcagaaag cagattaaag ggcgttgcga tgggcaaaga atataaaact ctcattaaca
     4981 aagcacttga acgattttat tttcgcttaa gtgcatcagg cgctcatgct gaacatgcag
     5041 cccgtgactc attgaccagg gcaatccgga gtttgtatga cgttgctttt tacgctgatg
     5101 atctggatgc acttaacgaa ctttccgagc tgatctgtgc cgcagaatgc ggggaacaca
     5161 ttgaaccata taagctgggg aatatcgcat gagtatattt atctcatggc ttgttctgat
     5221 tatttcggtg gcctgcgcca ttgggattat gcgaattatt cattcagtaa aaaagattga
     5281 acgctttttc actgacgaat aacagcttaa acaaaacacc agattaaacc cgaaaacctg
     5341 aaaactatcc gcattcgcgg aggtattcgc acacgtaaat aacggagata taaaatgaac
     5401 ggaaaagaag aaggcattat cgacacatta aaaaaaatat cagaagcgga agatgaaatg
     5461 gctaaagatg ccgtgaagcg tagccaacat atggcagcac ttcacgcact gaccattgca
     5521 aaaatcaccg ctgacgcagc caaaattatt gaggaacaga gcaaagaaat cgacactctt
     5581 aaaacacagt caacagttgc agccatgaat ccgtccagca ttggacgctg catttatatt
     5641 cttggttcag caatgatgct gcaatacacc attattgccg aactgcacgg caaatacctg
     5701 ataacgcctt accacacaaa agagtcagag cttctgacaa atctccgcct gatagaacgc
     5761 tctcaagctg tattcattga tgacgcgcaa cgtgccgtat ttaacgcata gggttactgg
     5821 acaaaggggg cgcaatggca attaagcatt tttccgtcgt tcgctttacc tccagagggc
     5881 gcgaatacga ggtcgacgaa cgcctgatta ccactatcga caaacatcgt tcggaaaagg
     5941 atgcacacca catctacctc actgacggca cttacttctg cgccaccaac gtggcgcgag
     6001 taaatcttat ccgacaggta caggagccac gcagatgacc attctggact acatcgctac
     6061 tcatccgggg tgtagcggcg gagagatcgc cgcagcactg aataccccaa ccacagccat
     6121 taatgctgag ttacgccgac tttggcgcgg cggcttagtc atcagaacaa accgcagcac
     6181 aggtggtcgc gctcgcaaaa caggaggcca ggcttcttac cacgtaaacc cgatgccgtt
     6241 cgggtgtagc aatccactta ccaacatgtt taaccagcta ctgaaggaag ccagagcatg
     6301 agcaccatca accaccagaa gctacgcgaa ctggcatttg ccctgcaacg aatggcaacg
     6361 cctcaaaaat tactggcatt tcgcgcaatg ctctcgccgt ctgctgtgct ggcactgctg
     6421 gatcagctgg agcacgccag aaccacggct cctgccattc gcctcacgct ccatcatgaa
     6481 atcgctgatt tctgcgcaac gctgggggca cctggcgaac cggaaacgcc ggaagcaatg
     6541 cagcaagagc tgctgcaacg catcgataac gtttttgatt tttttctgaa ccagtaagaa
     6601 accagaacat gcacacacaa aaaaaccgct tgccatgccg caatcggtca ggttacattt
     6661 ccgctgcacc tcataaaacg ggtgccggga ttctcaaccc gatacagagc aaagcgcata
     6721 accgcgcaag cggttttttt gtgcgtactg tattgccacg tctttttcgc gtcagaatta
     6781 tggcggggcg tacggggccg acttcggtcg ggccggattc tttgctctcc ggtgttgaga
     6841 accctgtacg tctcgccacc ccgagattct caactctgga tggtgagtta tttctatcac
     6901 cgagcaaaga ggccacacca tggcagaccg caaacagcac cgcgctatcg cggagcgtcg
     6961 tcacatccag actgaaatca accgcagact ttcccgcgca tcacgcgtcg cgcaaatcat
     7021 gcacatcaat atgctgcatg agcgcagcca cgcactatca aacatttatt ccgcctctgt
     7081 tttcagctat ctggcggatg atctgcacga gcttcaacag ctcatccagc agcaaaacaa
     7141 actccattaa ttcctgttcc gggcctttcc tgcaccttgc ggcgggaggc cttcgcacat
     7201 ctgtagtaaa gagaattgca gcatgattga cgctcatgac ttcacaagat gggtgcgcac
     7261 acaggacacc cgtctggctc ccgttcttca ggggttattt gatctctaca tccgtggtcg
     7321 tgacaacaga gcacgcacca caaaaccgga gaatgcagac accctttatt tcacagtaga
     7381 cgactgctac cgcgtggatt tcacaccaca cggactggcg ttgcactgcc tgacaccgca
     7441 cggcgaatca ctgctggcgt attacgactc cccggcctcc gtatttgcag caatgctggc
     7501 gcatcgcact gctggcgggt gtgcctcgct gagtgaatac accgctgaat ttaaccgcct
     7561 ttccgccctc ttctcgcagg agtggcagcg cgtgacggga taccagccat gagtgcgttt
     7621 gcatggagct ggaatgaacc acgaccagct attgatccgg ccagatttac ggagcgcagg
     7681 caggaaactg aaaccgacct gcaacgcgcc atccgttact accttgaggc ggacaaaagg
     7741 gcacaggaag aacaggaagc gaaggaagaa gcctttttcg cacaatccgc catgggtaaa
     7801 aaactcatgg catcccttga ggaagccgga cagcgtgaaa agctggcaca gagcatcatc
     7861 agtaagcgcc gggcaacaga acaagacccg gtagcccgtg cctttgccac actgaaggcg
     7921 cttcccgttt atctgcgaga acctctgagc cgccacctct ctttcctgcg caagaagcag
     7981 gaagccgatc gtcagaaagg caaaaagagc tggcaggcgg aacgctatac acgcggaacc
     8041 ctgcgcaaaa tattcgaacg tctggatcgc actgacgggc gctggctgac acctggttat
     8101 cgctcccttg ccggacgcga acgcctggac gatttgcttt acctgccgca gctcaacaaa
     8161 caccagatac agacgctggc caccatgacg gcggcgatgt tcagcagcac cttcgaaaaa
     8221 ctctgcgatg gctttggcgc gaccgatggc gaactgacca tggatgtaac gctgaaggcg
     8281 tatcagatgc tggcccgcat agcgttacac ctgcacatca tgcctccaca ttatgacgca
     8341 ctgacaacag ataaagaccg gaggaacgaa ccggacacgg agctgctgcc gggcgcaatc
     8401 cttcgcctga cctgtacgga atggtggaaa cgcaaactgt ggctgttacg ttgcgagtgg
     8461 agagaagaac aactccgcgc cgcctgtctg gtttccagaa aaacatcgcc ctatctgagc
     8521 caggacgcat taagcgagtt tcgcgcgcag cgcgagaaaa cacgcgattt cctgaaaagt
     8581 ttcatgctgg aaaacgaaga tgggttcacg attgatctcg agacagtgta ttacgcggga
     8641 gtaagtaacc cggttcaccg taaggcagaa atgatggcca ccatgaaggg gctggaactt
     8701 ctggccgaag cccgtggcga caaagcggtg tttctgactg tcacctgccc gtcaaaatac
     8761 cacgccacaa cagagaacgg tcatccgaat cccaaatgga acggggccac catgcgcgac
     8821 tccagcgatt acctggttaa cacgtttttt gcggcggtcc gcaagaaact gaaccgcgac
     8881 ggcctgcgct ggtatggcat ccgcacggtg gagcctcacc atgacggcac cgtgcactgg
     8941 catatgatgg tctttgctca tccggaagaa atcgacacta ttgtgtccca cacccgcgat
     9001 attgccattc aggaagatcg tcacgagctg ggtgatgata ttaccccacg ctttaaggcg
     9061 gagtacgtcg acggctcaaa aggcacaccg accagctaca tcgccaccta catcgggaaa
     9121 aacctggaca gccgtgccgt ggatggcatc gacccgaaaa cgggcaagcc acgcgttgac
     9181 cacgaaaccg gaaaatcaat ggccgagagc gtggaacgcg ccatcggctg ggcgcgcctt
     9241 caccgggtcc gccagttcca gttctttggc atcccctccc gccaggtatg gcgtgaactg
     9301 cgccgccttg ccagccagat ggcacgcaac ccggaaggcc cgcaacggct gaaggatgac
     9361 gcaatggatg cggttcttgc cgccgctgat gccggatgtt ttgccaccta catagagaaa
     9421 cagggcggcg tacttgttcc acgcaaagac tacctgattc gcaccgccta cgacctcgcc
     9481 gatgagctga acgattacgg cgaacagagc gtacagattt acgggatctg gtcaccactc
     9541 atcggggaat cctcccgtgt gtgcacgcat ccggataact ggaagctggt aagacgcaaa
     9601 ccggaagcgg aagacagtgc ccgcgaaaat ggttttgacc ttcagggcgg ccctgccgcc
     9661 ccttggactc gtggcaataa ctgtccccgt gtacaggaaa cggacaacaa cgggacagaa
     9721 cagccggaag aacggccaac accgcggccg cagcttcctg acggcgttga cgtgaatgaa
     9781 tggatgcgct cactgaaacg gcacgaacgc cgggcgctga tgcattcgct gcgtgacaaa
     9841 caggcaaaaa acagcagtga tgaaatgcag aactggacac agagccgcaa acagccacgg
     9901 cctttgcctg ataaccacga gttactcgct aaagaatggc gggagtctgc tgaatctctc
     9961 ggcctgcata tcggtgaaca gcagatgcag cacctgttac ggggcggcag tctgtacgtt
    10021 gacggcagca tcattgcacc gcagggattt gaaattgtac gcaaaccaga tacccgcccg
    10081 gacagccgaa tcacgcaact ctggcagcgc ctgagccgta atcacggcgt aagcagcacg
    10141 gagatccgcc ataacccggt cgccagctat ctggaacagc tgggggcatc agaccccgaa
    10201 gccgccgcac acctggcatc cacacttcag caggaccaga acaccatgaa aacccccgtt
    10261 accgtgcttt ctgacatgct gcgcgccatc cgtgacgcag agcacgcaca gagaatcagt
    10321 gaaaccactg aacgcgccca ccgcaaagca gacctgctgc ggggtagcct gaccagtgga
    10381 aacaaaaaac agacagaaac gggactcaca aatcccgtaa atgagcaaaa aacgtgccgc
    10441 gatatatgaa gcgcgcacaa aacaggcaaa agcgggattt aaaaatcctg taaccgatta
    10501 attaatcaac ataaggaaaa gcgacatgaa aatttgtatc gacgacggct ccaccaacat
    10561 caagctggca tggactgaga acggcgaacg ccgcaacgcc atcagcccga acagcttcaa
    10621 gtcggaatgg tctgcgccgt tcggtggcac gcagcccgcg aactacatgc ttgatggcgt
    10681 gcgctatggt tttgatccgg tcagcgatcg ctttgtccag acgaccgaca cgcaatacca
    10741 gtacagcgat gtgaatataa ttgccattca tcacgcgctg gtcaaatcag gcatcacacc
    10801 acaggaagtg gatgtggttg tcaccctgcc actaagcgag tatttcgata caaacgcaca
    10861 gccggacatg gccaacatca accgcaaaaa agcgaacgtc atgcgcccgg tggagtacca
    10921 gaacggcgaa gcattcacta tccgtaacgt acgggttatg cctgaatcca ttccggctgg
    10981 ctttaaggca ctggctgaca tgagtccgtt tgaatccctg ctgattgtgg atttgggcgg
    11041 aaccacgctg gatgtggcaa aggttcaggg gcaactggca ggtatcagcc aggtgttttg
    11101 cgatccacac gtgggcgttt ctctgatggc cgatgccgta ctgtcggtga tggccactaa
    11161 cggtatgcgc accagtcacc acatcgccaa taccattatc gaacatcgcc atgatgaagc
    11221 ctggctgcgc cagcacatcc acaatgacgc gcattacgcc agcctgatgg cggtaattcg
    11281 tgaaaaggaa gaaacactga aacaacgcgt gatccgcgcg ctggcgggtt tttcgggtta
    11341 cgggcgggtg atggttgtcg gtggaggggc ggagattgtg gcacccgcta tccgcgaagc
    11401 ctgcggagtt aatgcgactt tcatcgcgga cggggtgcca cagtttgctc tggttaatgg
    11461 gctgtacgca atggacaagg agtaaaccaa tgacgactcc aaccagacgg ataagtttct
    11521 atctgaagcc caccgccgtc aagaacgaag gagaagcatg cgcctggctg gacagcctta
    11581 caccagaagc ccgcaaaagc ggccaacgcg tggcttttct ggccgggctg gcacttctga
    11641 aaatgaatcc ggcagaggct tatcgactgg ctgcatgggc tggtgatgaa gcgttatcag
    11701 tgacacaaac caggacagaa cgccccgcat cacagccagt atcaaccgca cagataacca
    11761 gtcagatggc cggaaatatc cgggcgttat ttcctgaata acacaacatc agggcgagtt
    11821 cgccctgctc tccacctcag aacataaaca aggagaacga cttaatgagc gaaatcaact
    11881 atcaggcatt acgtgaacgt tattcacctg tgccagtacc gaaatgccct atttgcggcg
    11941 aggaaatgtc aattcagcga atatctggag cacaggttgt ttatgcctgc tccggttatg
    12001 gtgatgatgg agatttcaaa attggccgaa ctcttgctga cgaacattat gaaaaatcac
    12061 gcgtaacagt gttggatgtc ggggatcctg aagtattggc gctacttgat tggctggaaa
    12121 ccaaagacaa ccgaatcgct gaactggaaa aaatcgccac tgactatgca cttaaattcc
    12181 agaaagcaca ggacgcatta aagcatgccg ctttgctgca tagcaggacg gcgcaacaaa
    12241 caaataattt tgcagtatcg cttccggaca taagcgaata tttcattaat gacgtatttc
    12301 agcccttgcg atacgagcgg gatgttgaaa gagccatcat aaaggctggc ggaaaagcat
    12361 tgtggcagga gaaacacgag gacagaacgc atcagtcatg cgatgtaaat tgtggatggt
    12421 ttagcccact gacgacagat aaaaataaca cctgatcccc ctcaaaccag ggcgataatc
    12481 gcacatcgcc ctgctgcaca atagtgcaca aatttgcaca atttttttga acgaattttt
    12541 gcccttccag cccgcgtggc ggctggatcc gtcaaggatc cgtgcgtgca caaaaaaacg
    12601 cgctttttct gcgcgcaggt gacgggggaa cagcccgcgt ttcagggggt aaatagcgtt
    12661 cccttaacga tgtcgcagcg acacgacaga atggccgtat ttctcacgct gagcgtgaaa
    12721 aatacgtgag ggattctgat ttgatggggt gaaaggtaag gccgtcaaaa tcgcactgag
    12781 gcggcgagaa catgcagtca acgcggtggg attgcgtaag agtctgaccg tcgatggtgg
    12841 cgataaactg gaaggcgtcg tgaaattatc tgactgatac aggagctgga gagtcggggc
    12901 ataaattttt tatgccccgg cgaagcagca gacaagcgaa gcgcgtcagg atgtgggctg
    12961 gatgtctaac agtgcgtaag ggttaaagcg gatcacctct tcgccaagcc agtcattgat
    13021 gtgcttcatg gcctccatga cgggcatcag ctcgttaatt gcgtaaaccc gcgcggcctt
    13081 ctccacatca ccaaacgcac ttttttcacc cggcatcgcc cccatcagtt gcggcggaac
    13141 gcggtgcgca gccagcacat catcacggga tgccgcctta acattcatga actcatcctt
    13201 tgcggtgatc tgctggaacg gcaaaatttg caccccctct ttgcccccgt tgggcgcatg
    13261 aatgagcacg tttttaaacg caccaccacc acgcgcaccc tgtagcgttt ctttcaggga
    13321 gtccatgctt tcgcggttta cctgcgctgc accgatgtag atgatgcacc cggcgtggga
    13381 tccgttgtcg taatacagtt ttctgaacat gtccgccgaa tgagacaggc tggccgagag
    13441 taatgcgccg agatattccg gcatgccgta gatttcctgg ttaatgtcag gattcatcag
    13501 gtgacacact ttgcctgggc gaaactgaaa cgcatccttg ccatcctgca cataccacca
    13561 tgattcaaga tcgcttccgc gtcgcatgta tttcgccagg gcgtgccgta atttaagcgg
    13621 ttcgccgagc atattgcttc gaagctcaag gaatgcgtta ccgaacacaa accagtccag
    13681 cgccagcgcc gagaaatcct gccgggaaag cagcgggtgc gggatgtagc aaccgagcaa
    13741 tacattgcgc ttaaagtaaa gcgcagactg atgccaggac gtttgccggg cagctcttgc
    13801 cagaccgtac cagtccaccg gggtttcata ccaccgcccg ttatcagcac agtacatatt
    13861 gtccagcagg tcatgcccgg tcaggcgata aggaccatca aatgtgaatg cactgagcga
    13921 tgattctttc ctgagcgcat cagcgagatc aatgcgtgaa ctcatgcgca cttttttatt
    13981 ttttctgctc atcagaactc cataaccgtg aaacgctcgt tttctccttc gccgccaatc
    14041 ggttcgttaa tgacagcaag catggttgcc cacgcaaggt cgccgtggct gatccccctc
    14101 gctcggtccg tttcgtaagt gataaagccg cccggtgttt tcaccttacg cacggcgtta
    14161 aaggccgcga ccagctcgcg ttcggcgcga tcgtattccc accgcccggc acgcattatt
    14221 tgcagcattt tcagtaccag cgaccgtttt gatgacagcg tgaaggtgta cggaatagcg
    14281 gcagggaaaa accgtttcac tatctgataa acagcctccc cgttcccgcc cgtcacatca
    14341 atgccgatgt gttccacgtt gtagcgacac gtgaactctt caatgactct ggcctgttct
    14401 tcaaactcca gcccctgaac gcgtcgcgtc tccaccgttc gaaaacggcc accaggaaca
    14461 gacggaggaa ccaccacgg
//
LOCUS       DAFATA010000107        14406 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFATA010000107 DAFATA010000000
VERSION     DAFATA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.108"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..375
                     /locus_tag="LGJ20_003902"
     CDS             <1..375
                     /locus_tag="LGJ20_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK2387908.1"
                     /translation="IAHFARMTSVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDA
                     HTYTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIG
                     KPPVFYRADSEWAALFASLKSI"
     gene            421..1806
                     /locus_tag="LGJ20_003903"
     CDS             421..1806
                     /locus_tag="LGJ20_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387909.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDFLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            1849..3252
                     /locus_tag="LGJ20_003904"
     CDS             1849..3252
                     /locus_tag="LGJ20_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387910.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            3320..4012
                     /gene="ompL"
                     /locus_tag="LGJ20_003905"
     CDS             3320..4012
                     /gene="ompL"
                     /locus_tag="LGJ20_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBK2387911.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(4103..5368)
                     /locus_tag="LGJ20_003906"
     CDS             complement(4103..5368)
                     /locus_tag="LGJ20_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387912.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(5470..6450)
                     /locus_tag="LGJ20_003907"
     CDS             complement(5470..6450)
                     /locus_tag="LGJ20_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBK2387913.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(6458..7168)
                     /locus_tag="LGJ20_003908"
     CDS             complement(6458..7168)
                     /locus_tag="LGJ20_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBK2387914.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSSVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(7385..9208)
                     /gene="typA"
                     /locus_tag="LGJ20_003909"
     CDS             complement(7385..9208)
                     /gene="typA"
                     /locus_tag="LGJ20_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBK2387915.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            9581..10990
                     /gene="glnA"
                     /locus_tag="LGJ20_003910"
     CDS             9581..10990
                     /gene="glnA"
                     /locus_tag="LGJ20_003910"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBK2387916.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            11309..12358
                     /gene="glnL"
                     /locus_tag="LGJ20_003911"
     CDS             11309..12358
                     /gene="glnL"
                     /locus_tag="LGJ20_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBK2387917.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            12370..13779
                     /gene="glnG"
                     /locus_tag="LGJ20_003912"
     CDS             12370..13779
                     /gene="glnG"
                     /locus_tag="LGJ20_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBK2387918.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            13891..14001
                     /locus_tag="LGJ20_003913"
     CDS             13891..14001
                     /locus_tag="LGJ20_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBK2387919.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(14191..>14406)
                     /locus_tag="LGJ20_003914"
     CDS             complement(14191..>14406)
                     /locus_tag="LGJ20_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK2387920.1"
                     /translation="PIEKQWDLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGR
                     LLIRNICMCFDTYLRQKARMQQFSRVI"
BASE COUNT         3536 a         3609 c         3550 g         3711 t
ORIGIN      
        1 atcgcccatt ttgcccgtat gacctccgtc ttcaccaccc tgaaacctta cctgaaagag
       61 gctgtcgcgc tgaatgcgaa gtccggcctg ccggttatgc gcccgctgtt cctgcattac
      121 gaagacgatg cgcacactta caccctgaaa tatcagtacc tgttaggtcg cgacattctg
      181 gtcgctccgg tgcatgaaga aggccgtagc gactggacgc tctatctgcc ggaggataac
      241 tgggtccacg cctggacggg tgaagcgttc cggggcgggg aagttaccgt taatgcgccc
      301 atcggcaagc cgccggtctt ttatcgcgcc gatagcgaat gggcggcact gttcgcgtcg
      361 ttaaaaagca tctaatcaca tccgcccgtg gggaaactca tgggcaaagg gagaacatcg
      421 atgagtcaca tcacaacgga agatccagca actttacgcc tgccctttaa agagaaactc
      481 tcttacggta ttggcgacct ggcctctaac atcctgctgg atatcggtac gctttatctt
      541 ttgaagtttt ataccgactt tctggggctg ccaggcacct atggcggcat tatctttttg
      601 atttcaaaat tctttactgc gtttaccgat atgggtaccg gcattatgtt ggattcccga
      661 cgcaagatcg gtccaaaagg taagttccgt ccttttattc tgtatgcgtc attcccggtc
      721 accttactgg cgatcgccaa ctttgtcggc acaccgtttg atgtcaccgg taaaacggtg
      781 atggccacta ttctgtttat gctttacgga ctgtttttca gcatgatgaa ctgctcctac
      841 ggcgcaatgg tcccggcaat caccaaaaac cccaacgaac gcgcctcact ggcggcatgg
      901 cgtcagggcg gcgctacgct gggcctgctg ctgtgcacgg tgggattcgt gccagttatg
      961 aatcttatcg aaggtaatca gaaacttggc tatatcttcg ccgccacgct gttttcactg
     1021 ttcggcctgc tgtttatgtg gatctgctac tcaggcgtga aagagcgtta tgtcgaaacc
     1081 caaccaacca atccggcgca aaagcctggc ctgttgcaat ctttccgcgc aattgccggt
     1141 aaccgcccac tgttcattct gtgtattgcc aacctctgca ccttaggggc gtttaacgtc
     1201 aagctcgcca ttcaggtcta ttacacccag tacgtactta acgatcccat cctgttgtcg
     1261 tatatgggat ttttcagcat gggctgtatt ttcatcggcg tgttcctgat gcctggcgca
     1321 gtcagacgtt ttggtaagaa gaaggtctat atcggcggcc tgctgatttg ggtgctgggc
     1381 gatctgctca actatttctt cggcggcggc tcggtcagct ttgtggcgtt ctcctgcctg
     1441 gcgttcttcg gctcagcgtt tgttaacagc ctgaactggg cgctggtttc cgacaccgtc
     1501 gagtacggcg agtggcgcac cggcgtgcgt tcggaaggca cggtctacac cggcttcacc
     1561 ttctttcgca aagtgtctca ggcgctggct ggtttcttcc ccggctggat gctgacgcaa
     1621 attggctatg tgccgaacgt cgcacaggct gaccacacca ttgaagggtt acgccagttg
     1681 atcttcatct acccaagcgc actggcggta gtcaccattg tggcgatggg ttgcttctac
     1741 agcctgaacg agaagatgta tgtccgcatt gtggaagaga tagaagcccg taaacgcacg
     1801 gcgtaataat aataaacgac gccctgcggg gcgttataag gagtgattat gtctgaccat
     1861 aatccactga cattaaaact gaatctgcgg gaaaaaatcg cctatggtat gggcgacgtc
     1921 ggttcgaatt taatgctctg catcggtact ctgtatctcc tcaaatttta taccgatgaa
     1981 ttagggatgc ctgcttacta tggcgggatt atctttctgg tggcgaagtt ttttaccgcg
     2041 tttaccgata tgctcaccgg atttttactc gactcgcgga aaaatattgg gcctaaaggc
     2101 aaatttcgtc cctttatttt atatgcagca gttccggcgg cgttaattgc aacgctccag
     2161 tttatcgcca ccaccttttg tttgccggtt aaaacgacga ttgccaccgc gctgtttatg
     2221 atgtttggac tttcatacag tctgatgaac tgctcgtatg gtgcgatgat cccggcaatt
     2281 accaaaaacc cgaatgagcg cgcgcagctc gcggcttacc gccagggtgg tgcaaccata
     2341 gggctattga tttgtaccgt ggcgtttatt ccgttgcagt cgcttttttc tgactcaacc
     2401 gtcggttatg cctgtgcggc acttatgttc tccattggcg gctttatttt tatgatgctg
     2461 tgctacagag gcgtcaaaga gcattatgtg gacacagcgc caaccggaca taaagccagt
     2521 attctcaaat ctttttgcgc gatatttcgt aatccaccat tgctggtttt atgcattgct
     2581 aacctgtgta ctctggcggc atttaatatc aaactggcga ttcaggtcta ttacacccag
     2641 tatgtgctga atgatattaa tttattgtcg tggatgggat tcttcagtat gggatgcatc
     2701 ctcgtcggcg tattactggt gccagtaact gtaaaatgtt ttggtaaaaa acaggtttat
     2761 ttagctggca tggtgctgtg ggcggtgggt gatatactga attatttctg gggaagtaac
     2821 tctttcactt tcgtcatgtt ctcttgtgtc gccttttttg gcacggcctt tgtaaacagc
     2881 ctgaactggg cactggtacc agataccgta gattacggtg aatggaaaac cggtattcgt
     2941 gccgaaggtt ctgtttatac cggttatacc ttctttcgta aaatttctgc cgcacttgct
     3001 ggcttcttgc caggcattat gctgacgcaa attggttatg ttcccaacat cgcgcaaagc
     3061 gatgcgacat tacagggttt gcgtcagctc atttttatct ggccttgtgc actggcaatt
     3121 attgctgcat tgacgatggg attcttttac acactcaatg aaaaacggtt tgcattgatt
     3181 attgaggaaa tcaaccaacg caaaaataaa gagatggcga cagaagaaaa aacggcttcc
     3241 gtaacgttat aaatgtcaat agccgctatt tccatcctgg tgggtggcgg cctccctacg
     3301 tttaaaaaat ggacttatta tgaaaaagat taatgcaata attttattat cttctctgac
     3361 ctctgcctcg gtatttgctg gcgcatacgt cgaaaacagg gaggcatata atcttgcctc
     3421 cgatcagggc gaggttatgc tacgtgtggg ttataacttc gatatgggcg cgggtattat
     3481 gttaaccaat acctacacct ttcagcgaga agatgaacta aaacatggct ataatgaaat
     3541 tgaaggttgg tatcctttat tcaaaccaac cgataaatta accatccagc ccggtggctt
     3601 aattaatgat aagagtatcg gttcgggtgg tgcagtgtat ctggacgtca actataaatt
     3661 tgtaccatgg tttaatctga cagtacgaaa tcgctataac cataataact atagttcaac
     3721 agatttgagc ggggaactgg ataataatga cacctatgaa attggcacct actggaattt
     3781 taaaatcacc gataaatttt cctatacatt tgagccacat tacttcatgc gagtgaatga
     3841 ctttaatagc agcaatggaa aagatcatca ctgggaaatc actaatacct tccgttatcg
     3901 cattaatgaa cactggctgc cttatttcga attacgttgg ttagatcgta atgtcgaacc
     3961 gtaccaccgc gagcaaaacc agattcgtat cgggacgaag tatttcttct gatgtgaaac
     4021 tgccggatgc ggcgtaaacg ccttatccgg cctacggttg atacggtttt cgttggccgg
     4081 attaagcaca tccggccatt aatcaagctt cagccgccag catctctttc ttacgttggc
     4141 taattcgctt aaccagcatt acggaaacca ccgcaccaca gaatgcgaag caggccataa
     4201 tgccaaacac gattttgtag ccaataatcc ccggatttaa atccagaatg tagccataca
     4261 gactgaagca gaacatcgcc ggagcgtaac caataaagct acccaacgcc atcgccgcgc
     4321 ctgttttatt ttcagcaatt tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg
     4381 taaagactat cgcgccaaag cccagcgtac atgccatccc taaatagacc ggcatacttt
     4441 cgtgcggcag cataatcaac agtacgagcg cagcggtact gatgataaag gtgtagcata
     4501 gatatttact cggcgatttc aggatcttat ctgaaatcat gccaccaatc ggtccaccaa
     4561 tcattttcag acagtattgg ttaatgatgc cgtaagcccc caccagcgca acgggcaata
     4621 gatagatgtt tttcaggaat ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga
     4681 agaagacgtt aaaagcgata agccagatag ttttgtcttt cagcaccgag gtcatactgg
     4741 tgtttttcga tgctccgtct tcttttttca cctcgacgga cggtgcctct tctttgtcat
     4801 tcagaacaaa gaaaatgata atgcctacgg caatcacaat aagggaatag aaccagatgc
     4861 ctgctttaaa acccaataag ccactgccaa accaggtaaa tactgccaac gcagaaaatg
     4921 ccaccacggt atcgacaatg ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt
     4981 gttcgctatt gcccaatcga cttaccgact tgagcaagac cggccagttc atcatgtcgc
     5041 aagtaacgcc aaatagcgcc cagacaaaga ggatgcccca ataccccggc attgtcgtca
     5101 aataaacacc cagtaatcct gtcgcaatga gtgagaatga catggtgtat ctgcgtggta
     5161 gtttatcggc aaaataaata gacagaaaaa aacccactgt cgtgacaaat gagtttaccg
     5221 acatagcatt accaatttga ccattggtca aatggaaata ttcctgcatc gggatataaa
     5281 acgcatcttt cagcgacggt aatttataaa ttgtaccgcc acacaatgtt aatagactaa
     5341 ataacgccca tttctttttc gtgagcataa catcccctaa gcgattttta aaatcactgt
     5401 tttaaaggca ataagatacc gaccattcat atggaatggt tttattaact gcgtatgagt
     5461 taaaacttat tagtggttca gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt
     5521 acgcacatga tttatctgat taattgcatc ttctttttct ttacgtaacc gcgcatccag
     5581 taaatgattt tctggcaatg gtgtttcact catctggcga taagggatcc acggattatc
     5641 atcttcgtct tcaaagcgga acgccggcgc atagtaatca acctcttctt ccaggccata
     5701 tgccgtcacc tgcggctcat catcacccag gcatataagg tgcgttaata acgctttgaa
     5761 cggcatatca ccctgacctg aaatacaggc tttatgaccc aggccacccg gttctttaac
     5821 gatcaaggca tctttgatat ggacctgggt aatatgcggc gccatcgttt ttaaagcgtc
     5881 gatgggatgc tcatttgcat tgatcatgtt cgcaaaatca aacagtaagg aaagagattc
     5941 catctcactt tctttgacca gcgacaccag ctcatggctt tttaaatctt catgctgttc
     6001 gatcgtaaaa gtcaggccgc tgtcctgata cgtttcccgt acataggcaa tgtcgttagc
     6061 gatgatcgat aatacgtcgc gcaaattacc ttcataacgt ggataaaaac gtacggacga
     6121 tgccccagtt ttcaacgcaa tggcgacggc ttcgtcaata gatgccttat ctgaggcgct
     6181 ggtttcaata tgaatatcaa ggttcagacg gcgggcttta tctccaaagg cagagagttc
     6241 tttatcgtcc atattaccaa gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc
     6301 acgtagatta ttttcacttg cgatatcaag taaatcagca ggcaacactc tttccagccg
     6361 catatttaaa tgaaaggcat aagcatgtaa ataaagagga agagtgtcga cacgttgtag
     6421 aatctttctt gcgttattta ttgtcaccat aaaacaccta cagattaatg atgaggtcta
     6481 cggcctcaaa tcgccaatat tcaatatcta tctggacaat attgccttgc tggtcggcgc
     6541 gatgcttttc aattaagatc gctggcatac ccgcagaacc gccaagatac ttgctgatat
     6601 cgcccggcat tctgacgggt ttgaaaatca atttttttac taccgtctct ttttggtagc
     6661 acttttccca gactgaagaa aatgagtggt tttcaagttg ttcaataaaa cccggagcaa
     6721 cttcaggatt aatatatgtt tcgtgataga aaactttatg tccttccagc gccccccagc
     6781 cggtgatgcg atagagttca gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg
     6841 gaatatcgct ggtacggctt ttctcggtaa acccccagga aggttcccgc ccttgctcaa
     6901 ttgcggcccg ctgaaagctc gccgacagct ccgggctgta attaaatcgc ggctgagtca
     6961 cgaaccaccc tttacgatcc ttacggaaga ttttggattc tgcttcgagg ttcagcaacg
     7021 cctggcgcag cgtcatacgt ttaatgccca gcagctctcc cagctctctt tctgaaggga
     7081 gttttccacc cggcgtagtg atgccatctt ttaaccaccg atccagtttc tcttttgcat
     7141 tttctacggt ggattgattt cccgtcattg tttaatcctt ttggtttaaa ccagatgagg
     7201 catcttacgc ttacgccaaa aaagaaagca attaacggcg tatgactttg cagataattg
     7261 tgacaaagct ctctattgac gtaatcccag acttataagg gagatactcg cattggtgtg
     7321 cgctcacgac aggtaaaaaa aaacctgcca gcgatggcag gttttttata actaaaaaac
     7381 gaaattaatc gtctttcggt gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac
     7441 gtttacgaat acggatagag gtcggagtca cttctaccag ttcgtcatca tcgatgaact
     7501 ccagagcttg ttccagagtc atgcggatag gcggaaccag aacaacggct tcgtcagtac
     7561 cggaagcacg catgttggtc agtttcttac cggtcaggca gtttacagtc aggtcgttag
     7621 agcggctatg aataccgata atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca
     7681 gcttaccgcg atcctgcaga ccgaacagcg cgaacgcgac cgctttaccc tgaccgttag
     7741 agatcagtac gccgttctga cgctgaccca cttcacccgg acgtacgtcg tcgtagtggc
     7801 tgaaggtgga gtacagcaga ccagtaccgg aagtcatggt catgaactca gaacggaagc
     7861 caatcagacc acggcttggg atcacgtagt cgagacgtac gcggccttta ccgtctggat
     7921 tcatgttttt caggtcgcct ttacgttcgc ccagcgcctg cattacagaa ccctgatgct
     7981 gttcttcaac gtccagagtc acgttttcat atggctcttg tttacgaccg tcgatttcac
     8041 ggaagataac tttcggacgg gataccgcca gttcgaaacc ttcacgacgc atgttttcga
     8101 tcagaacaga caggtgtagt tcgccacgac cagaaacgcg gaacgcatca gcgtcttcgg
     8161 tttcttctac gcgcagcgca acgttgtgta ccagttcttt gttcagacga tccaggatct
     8221 gacgagacgt tacgaactta ccttctttac cgcagaacgg cgaggtgtta acgcagaaga
     8281 acatagaaac ggtcggctca tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt
     8341 cgcaaacggt gtcagaaatg ttcagttcgc caaggcccgt gatcgcaacg atatcgccag
     8401 cttccgccag gtcggtttcg atacgttcca gaccgaggtg gcccagcact ttaccgactt
     8461 tcgcgttgcg ggttttgcct tcgctatcga tgatagtgac ctgctggttc ggcttcactt
     8521 taccgcgctt gatgcggcca atgccgataa cgccaacata gctgttgtaa tcgagctgag
     8581 aaatctgcat ctggaacgga ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa
     8641 tcgcctggta cagcggggtc atgtcttccg ccatatcttc gtggtccaga cccgcgatac
     8701 cgttcagcgc agaagcgtaa acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt
     8761 taacgaacag atcgaatacc tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa
     8821 ctttgttgat aacaacaatc ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc
     8881 gcgtttgcgg catcgggccg tcaaatgcgt caaccaccag cagcactgag tctaccatgg
     8941 acattacacg ttcaacttca ccaccgaagt cggcgtgccc cggggtatca acgatgttga
     9001 tacggtaatc attccatttg atagcggtgt ttttcgcgag gatggtaatc ccacgctctt
     9061 tctccaaatc gttggagtcc atcacgcgct cttgggtttc ggcacgagag tcgaacgtac
     9121 cggattgttg gagcagcttg tctaccaggg tggttttacc atggtctacg tgcgcgatga
     9181 tggcgatatt acgcaatttt tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt
     9241 attgtacacg gattaatcgc actacaaaac aggatcacaa acatcctccg caaacaagta
     9301 ttgcagagtc cctttgtgat cgctttcacg gagcataaaa agggttatcc aaaggtcatt
     9361 gcaccaacat ggtgcttaat gtttccattg aagcactata ttggtgcaac attcacatcg
     9421 tggtgcagcc cttttgcacg atggtgcgca tgataacgcc ttttaggggc aatttaaaag
     9481 ttggcacaga tttcgcttta tcttttttac ggcgacacgg ccaaaataat tgcagatttc
     9541 gttaccacga cgaccatgac caatccagga gagttaaagt atgtccgctg aacacgtact
     9601 gacgatgctg aacgagcacg aagtgaagtt tgttgatttg cgcttcaccg atactaaagg
     9661 taaagaacag cacgtcacta tccctgctca tcaggtgaat gctgaattct tcgaagaagg
     9721 caaaatgttt gacggctcct cgattggcgg ctggaaaggc attaacgagt ccgacatggt
     9781 gctgatgcca gacgcatcca ccgcagtgat tgacccgttc ttcgccgact ccaccctgat
     9841 tatccgttgc gacatccttg aacctggcac cctgcaaggc tatgaccgtg acccgcgctc
     9901 cattgcgaag cgcgccgaag attacctgcg ttccactggc attgccgaca ccgtactgtt
     9961 cgggccagaa cctgaattct tcctgttcga tgacatccgt ttcggatcat ctatctccgg
    10021 ttcccacgtt gctatcgacg atatcgaagg cgcatggaac tcctccaccc aatacgaagg
    10081 tggtaacaaa ggtcaccgtc cggcagtgaa aggcggttac ttcccggttc caccggtaga
    10141 ctcggctcag gatattcgtt ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt
    10201 tgaagcccat caccacgaag tagcgactgc tggtcagaac gaagtggcta cccgcttcaa
    10261 taccatgacc aaaaaagctg acgaaattca gatctacaaa tatgttgtgc acaacgtagc
    10321 gcaccgcttc ggtaaaaccg cgacctttat gccaaaaccg atgttcggtg ataacggctc
    10381 cggtatgcac tgccacatgt ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa
    10441 atacgcaggt ctgtctgagc aggcgctgta ctacattggc ggcgtaatca aacacgctaa
    10501 agcgattaac gccctggcaa acccgaccac caactcttat aagcgtctgg tcccgggcta
    10561 tgaagcaccg gtaatgctgg cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc
    10621 ggtggtttct tctccgaaag cacgtcgtat cgaagtacgt ttcccggatc cggcagctaa
    10681 cccgtacctg tgctttgctg ccctgctgat ggccggtctt gatggtatca agaacaagat
    10741 ccatccgggc gaagccatgg acaaaaacct gtatgacctg ccgccagaag aagcgaaaga
    10801 gatcccacag gttgcaggct ctctggaaga agcactgaac gaactggatc tggaccgcga
    10861 gttcctgaaa gccggtggcg tgttcactga cgaagcaatt gatgcgtaca tcgctctgcg
    10921 tcgcgaagaa gatgaccgcg tgcgtatgac tccgcatccg gtagagtttg agctgtacta
    10981 cagcgtctaa tagttgaagt tgtactaccc ggcgcaacaa cgccggaggt tcaaaccagg
    11041 cccatctggc gtaattgttg cagccagttt gaacacggac agcgcgcaga acccggagcg
    11101 tacactggta cgtgaggagt tcgagcactg cccaggttca aaatggcaaa taaaatagcc
    11161 tgatgggact ggtttttagt tgccgtggaa atttcagccc atctctgcat gggctttttt
    11221 ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa aaagctataa tgcactaaaa
    11281 tggtgcaacc tgttcaggag actgctttat ggcaacaggc acgcagcccg atgctgggca
    11341 gatcctcaac tcgctgatta acagtatttt gttaatcgat gacaacctgg cgatccatta
    11401 cgccaaccct gccgcgcaac aactgctcgc ccaaagctcc cgcaaattgt ttggtacacc
    11461 gttaccggaa ctgttgagct acttctcatt aaatatcgag ctgatgcaag aaagtctgga
    11521 ggcggggcaa ggttttaccg ataacgaagt gacgctggtc atcgacgggc gctcgcatat
    11581 cctttctgtg acggcccagc gtatgccgga cggcatgatc ctgctggaga tggctccgat
    11641 ggataaccag cgccgcttaa gtcaggaaca gctacagcac gcccagcagg ttgctgcccg
    11701 tgatttagtg cgcggcctgg cacatgagat taaaaatccg cttggcggtt tacgtggcgc
    11761 ggcgcagctg ctcagcaaag cgttacctga cccatcacta ctcgaatata ccaaagtgat
    11821 tatcgaacag gcggaccggc tgcgaaatct ggtcgaccgt ctgttggggc cgcagctgcc
    11881 cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa cgcgtggtaa cgctggtgtc
    11941 gatggaactg ccggacaacg tgcggttgat tcgtgattac gatcccagcc tgccggaact
    12001 ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat attgtgcgca atgcgctaca
    12061 ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc cgcaccgcgt ttcaactgac
    12121 cttacacggc gagcgctacc ggctggcggc gcggattgat gtggaagata acgggccggg
    12181 cattccgcct catttgcagg atacgctgtt ttacccgatg gtcagcggcc gcgaaggtgg
    12241 caccgggctt ggcttatcca tcgctcgtaa tttgattgat cagcattcag gcaaaattga
    12301 atttaccagt tggccagggc ataccgagtt ctcggtttac ctgcctatca ggaaataaag
    12361 gtgacgttta tgcaacgagg gatagtctgg gttgtcgatg acgatagttc catccgttgg
    12421 gtgcttgaac gtgcgctcgc tggggcaggt ttaacctgta cgacgtttga gaacggcgca
    12481 gaagtgctgg aggcgctggc gagcaaaacg ccggatgtgc tgctttcaga tatccgtatg
    12541 ccgggaatgg acgggctggc gctgctcaag cagattaaac agcgccatcc aatgcttccg
    12601 gtcatcatta tgaccgcaca ttccgatctg gatgctgccg tcagcgccta tcaacaaggg
    12661 gcgtttgatt atctgcccaa accgtttgat atcgacgaag cagtggcgct ggttgagcgc
    12721 gctatcagtc attaccagga acagcagcag ccgcgtaatg ttcagcttaa cggcccaacg
    12781 accgatatca tcggcgaagc gccagccatg caggacgtgt tccgtattat cggtcggctt
    12841 tcgcgttctt ctattagcgt gctgattaac ggcgaatccg gcaccggtaa agaactggtc
    12901 gctcatgccc tgcatcgcca cagtccgcgc gccaaagcgc cgtttatcgc gctgaatatg
    12961 gcagctatcc caaaagattt gatcgaatca gaactgtttg gccacgagaa aggcgcgttt
    13021 actggcgcga ataccattcg tcaggggcgt tttgaacagg ccgatggcgg tacattattc
    13081 ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc gtttgctgcg cgtgctggca
    13141 gacggtcagt tttaccgcgt tggcggctat gcgccggtga aagtggatgt gcggattatc
    13201 gctgccactc accagaatct cgaacagcga gtgcaggaag gtaagttccg tgaggatctg
    13261 ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc tgcgcgaacg tcgggaagat
    13321 attccccgtc tggcgcgcca ttttttacag gttgccgcgc gcgaactggg cgtagaagcg
    13381 aagttactgc atccggaaac cgaagctgct ctgacgcgtc tggcgtggcc aggcaacgtg
    13441 cgccagctgg aaaacacctg ccgctggcta acggtgatgg ccgccgggca ggaagtgttg
    13501 attcaggatt tgcccggcga actgtttgaa tcaacggttg cggagagtac ttcgcaaatg
    13561 caaccggaca gctgggcgac gcttcttgcg cagtgggcag acagagcgct gcgttccggt
    13621 catcaaaatc tgctttccga agcgcagcca gagctggagc ggacgttact gacgaccgcg
    13681 ttgcgacata cgcaggggca taaacaggaa gcggcgcggc tactcggctg gggccgcaac
    13741 accctgacgc gtaagttaaa agagctgggg atggagtgat tcacagcttg tgtgtaaaga
    13801 ttgattattg agcgcaaatt gctggtattt tacgctttac tgttccgata agttcagtat
    13861 gatcttgccc ggaaaacggg gagagtcatt atgctggaat caataattaa tctggtatcg
    13921 agtggcgcag ttgacagcca cacaccgcaa actgccgttg ctgccgtgct gtgtgccgca
    13981 atgattgggc tgtttagttg agtttcagat tgctgatgaa gtgctctttg ttcatgccgg
    14041 atgcggcgtg aacgccttat ccggcctaca aaagcaatat tttcaacagg ttttgtaggc
    14101 ctgataagcg tagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt
    14161 ctactttgta aacgaagcgc cattcactat ttaaatcacc cgtgagaact gctgcatccg
    14221 cgctttctgg cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc
    14281 tttcgccgtc acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg
    14341 ggcgagcagc ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc
    14401 aatagg
//
LOCUS       DAFATA010000108        14329 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFATA010000108 DAFATA010000000
VERSION     DAFATA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.109"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(285..497)
                     /gene="rpmE"
                     /locus_tag="LGJ20_003915"
     CDS             complement(285..497)
                     /gene="rpmE"
                     /locus_tag="LGJ20_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBK2387921.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            700..2898
                     /gene="priA"
                     /locus_tag="LGJ20_003916"
     CDS             700..2898
                     /gene="priA"
                     /locus_tag="LGJ20_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK2387922.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3054..4079
                     /gene="cytR"
                     /locus_tag="LGJ20_003917"
     CDS             3054..4079
                     /gene="cytR"
                     /locus_tag="LGJ20_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBK2387923.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4171..5130
                     /gene="ftsN"
                     /locus_tag="LGJ20_003918"
     CDS             4171..5130
                     /gene="ftsN"
                     /locus_tag="LGJ20_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBK2387924.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5223..5753
                     /gene="hslV"
                     /locus_tag="LGJ20_003919"
     CDS             5223..5753
                     /gene="hslV"
                     /locus_tag="LGJ20_003919"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBK2387925.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5763..7094
                     /gene="hslU"
                     /locus_tag="LGJ20_003920"
     CDS             5763..7094
                     /gene="hslU"
                     /locus_tag="LGJ20_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBK2387926.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7161..8087
                     /gene="menA"
                     /locus_tag="LGJ20_003921"
     CDS             7161..8087
                     /gene="menA"
                     /locus_tag="LGJ20_003921"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK2387927.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8180..8665
                     /gene="rraA"
                     /locus_tag="LGJ20_003922"
     CDS             8180..8665
                     /gene="rraA"
                     /locus_tag="LGJ20_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBK2387928.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8750..8995)
                     /gene="zapB"
                     /locus_tag="LGJ20_003923"
     CDS             complement(8750..8995)
                     /gene="zapB"
                     /locus_tag="LGJ20_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBK2387929.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9421..10266
                     /gene="glpF"
                     /locus_tag="LGJ20_003924"
     CDS             9421..10266
                     /gene="glpF"
                     /locus_tag="LGJ20_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBK2387930.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10289..11797
                     /gene="glpK"
                     /locus_tag="LGJ20_003925"
     CDS             10289..11797
                     /gene="glpK"
                     /locus_tag="LGJ20_003925"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK2387931.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11933..12943
                     /gene="glpX"
                     /locus_tag="LGJ20_003926"
     CDS             11933..12943
                     /gene="glpX"
                     /locus_tag="LGJ20_003926"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBK2387932.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13040..13786
                     /gene="fpr"
                     /locus_tag="LGJ20_003927"
     CDS             13040..13786
                     /gene="fpr"
                     /locus_tag="LGJ20_003927"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBK2387933.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13791..14219)
                     /gene="uspD"
                     /locus_tag="LGJ20_003928"
     CDS             complement(13791..14219)
                     /gene="uspD"
                     /locus_tag="LGJ20_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBK2387934.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14246..>14329)
                     /locus_tag="LGJ20_003929"
     CDS             complement(14246..>14329)
                     /locus_tag="LGJ20_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HBK2387935.1"
                     /translation="QVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         3436 a         3689 c         3879 g         3325 t
ORIGIN      
        1 ccgccggggc acaccgaaca ggccacgccg gtgggggcac cgatgcgcac cccggctgaa
       61 ccctggacaa tgctaccgcc atactgcgtc atgtcgccct gacgcgctgc cggttttccg
      121 ctcatcgtaa aatccttttg aaataaataa gaaccagaag cctagcgcca gtcaacaaca
      181 ggaaatgata atgagttaac accccggaat ttttcagaat ttataaaagg caaattacag
      241 gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc ggtcttattt gctgcccggg
      301 atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg tagcaacatc acgctgtttg
      361 ccagtgaaga acgggtggca cttgctgcac acgtcgaggt tcaggtcatg accaacggtg
      421 gagcggattt tcattacgtt accgcaagag cagctagcag taatttcttc gtatttcggg
      481 tgaatatctt ttttcatggg aaaacctcgg tttaaggccg cgtcgctctt ccagccctaa
      541 cgccagacac cacgcgatgt taaaagtata gcttcaatac gatcatttcg tacgaaggcg
      601 cgaaatcata cagaaattaa ccagcgtatg caaactgatc cgcactcttc tacggcaatg
      661 tgtatactaa cccaccgaat ttcaagtcag gatgatgcta tgcccgttgc ccacgttgcc
      721 ttgcccgttc cgcttcctcg tacctttgac tatctgctgc cagaaggcat gacggttaaa
      781 gccgggtgtc gcgtgcgcgt gccgtttggc aaacagcagg agcgcatcgg ggttgtggtg
      841 tcagttagcg atgtcagtga gctgccgctt aacgagctga aagcggtagt tgaagtgctg
      901 gacgttgagc cggtatttac tcactccgtc tggcggttac tgctctgggc ggcagattac
      961 tatcatcatc cgattggcga tgtgctgttt catgccttgc cgattttact gcgccagggg
     1021 cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca ctgaacaagg tcaggcggtg
     1081 gatcttaaca gcctgaaacg ttcgccaaag caacaacagg cgctggcggc gttacggcaa
     1141 ggcaaaatct ggcgcgacca ggtcgcggaa cttgaattta atgatgccgc gttgcaggcg
     1201 ctgcgcaaaa aaggtctgtg tgatttagca agtgaaacac cagagtttag cgactggcga
     1261 acgaactatg ccgtttctgg tgagcggttg cgattgaata ccgaacaagc caccgccgtt
     1321 ggcgcaattc atagcgcggc agatactttt tctgcctggc tgctggcggg cgttaccggt
     1381 tccggtaaaa cggaggttta tctcagtgta ctggaaaacg tgctcgctca gggcaaacag
     1441 gcgctggtga tggtgccgga aatcggcctg acaccgcaaa ctatcgcccg ttttcgtgaa
     1501 cgttttaatg cccccgtgga agttctgcat tccggcctga acgacagcga gcggctttcg
     1561 gcgtggttga aagcgaaaaa tggcgaggcg gcgattgtga tcggcacccg ctccgcgctg
     1621 tttacgccgt ttaaaaatct cggcgtgatt gtcattgatg aagagcacga cagctcctac
     1681 aagcagcagg aaggctggcg ctatcatgcc cgcgacctgg cggtgtatcg tgcgcatagc
     1741 gagcaaatcc cgattattct tggctccgca acgcccgcgc tggaaacgtt atgcaacgtc
     1801 cagcagaaaa aataccgcct gctgcgcctg acccgtcggg cagggaatgc gcgtccggca
     1861 attcaacatg tgctggattt aaaaggtcag aaggtgcagg caggtctggc tccggcgtta
     1921 atcacccgta tgcgccagca tttacaggcc aacaatcagg ttatcctctt tcttaatcgt
     1981 cggggttttg cgcctgcgct gctgtgccac gactgcggct ggatagccga atgcccacgt
     2041 tgcgatcact actacacgct gcatcaggcg caacagcatt tgcgctgtca ccactgtgac
     2101 agtcagcgtc cggtgccgcg ccagtgccct tcctgcggtt ccacgcacct ggtccccgtg
     2161 gggctgggca ccgaacagct tgaacagacg cttgcgccga tgttccccgg cgtgcccatt
     2221 tcacgtatcg accgcgatac caccagccgc aaaggggcgc tggaacagca actggcggaa
     2281 gtgcatcgcg gcggcgcgag gatcttgatt ggcacgcaaa tgctggcgaa aggtcaccac
     2341 tttccggatg tgacgctggt ggcgttactg gatgtggacg gcgcgctgtt ttctgccgat
     2401 tttcgctcgg cagaacgttt cgcccagctt tacacccagg tcgccggtcg tgccgggcgt
     2461 gcgggtaaac agggcgaagt ggtgctgcaa acgcaccatc cggaacatcc tctgttgcaa
     2521 acgttgctct ataaaggcta cgacgccttt gccgaacagg cgctagctga gcgacgcatg
     2581 atgcagctgc cgccgtggac cagccatgtg attgtgcgtg cggaagatca taacaatcag
     2641 catgcgccgc tgttcctgca acaactgcgt aatctgatcc tttccagccc gctggcagac
     2701 gacaagctgt gggttctcgg cccggttccg gctctggcac ctaagcgtgg tggccgctgg
     2761 cgctggcaga tcttgttgca gcacccttcc cgcgtgcgct tgcaacacat cattagtggt
     2821 acgctggcgc ttattaatac aataccggat tcccgcaagg tgaaatgggt gttggatgtc
     2881 gatcccatcg aaggctaatg cgcaaacgtt gcgaggcgga tcgaaaaatt caatattcat
     2941 cacacttttc atgaaaattc tgtaaccgtt ttcacgcgct atctgctaaa aatgttgccg
     3001 atgtgaagta aacatggatg tagtacgcct gacgtgccag gcgaggagtg agtgtgaaag
     3061 cgaagaagca ggaaactgcc gcgaccatga aagacgttgc cctcaaggca aaagtctcta
     3121 cagcgactgt ctcccgagca ttaatgaatc ccgataaagt ctcccaggcc acccgtaatc
     3181 gggttgaaaa agcggcccgg gaagtgggtt atttaccgca acctatgggg cgcaacgtca
     3241 agcgtaatga atcccgcacc attctggtga ttgtcccgga tatctgcgat cccttcttta
     3301 gcgaaattat tcgcggtatc gaagttacgg cggcaaatca cggatatctg gtgctgattg
     3361 gcgactgtgc gcatcaaaat cagcaggaaa aaacctttat cgatttgatc atcaccaagc
     3421 aaattgatgg catgttgctg ctgggctcaa ggctgccgtt tgatgccagc attgaggaac
     3481 agcgtaatct gccgccgatg gtgatggcga acgaatttgc accggaactg gagctgccta
     3541 ccgttcatat cgacaatctg accgccgcat ttgatgcagt aaattattta tatgagcaag
     3601 ggcataaacg gattggctgt atagccggtc ccgaagagat gccactgtgt cactatcgcc
     3661 tgcaaggcta tgttcaggcg ctgcgtcgct gcggcattat ggttgatccg caatacatcg
     3721 cccgtggcga cttcaccttc gaagccggaa gcaaagcgat gcagcagctg cttgatcttc
     3781 cacaaccgcc tactgctgtc ttctgccata gcgatgtgat ggcgctcggc gcactttctc
     3841 aggcaaaacg ccaggggctg aaagtcccgg aagacctttc cataatcggt tttgataaca
     3901 tcgacctgac gcaattttgt gatccgccgc tgacaaccat cgcgcagccg cgttacgaaa
     3961 tcggtcggga agctatgctg ttattgcttg atcaaatgca ggggcaacac gttggcagtg
     4021 gctctcgttt aatggactgc gaacttatca tccggggatc aacacgcgcg ttaccttaaa
     4081 gtaaaccttt aagactgccg tgactggtca aaggcccgcc gcttaagtaa catggcgggc
     4141 tgacgaacga ataaatacag cgaaacgata gtggcacaac gagattatgt acgccgcagc
     4201 caaccggcac cttcgcggcg aaaaaagagc acctcacgga aaaagcaacg aaatctgcct
     4261 gcggtttctc ccgctatggt cgctattgct gccgccgttc ttgtgacctt tatcggtggt
     4321 ctgtacttca ttacgcatca caagaaagaa gagtccgaga cgctgcaaag ccagaaagtg
     4381 accggtaacg gactaccacc aaaaccagaa gaacgctggc gctacattaa agagctggaa
     4441 agtcgccagc cgggtgtgcg tgcgcccaca gaaccttctg ccggtggtga agtgaaaacg
     4501 ccggagcaac tgacaccaga acaacgtcag cttcttgaac aaatgcaggc tgatatgcgc
     4561 cagcagccaa cgcagctggt tgaagttccg tggaatgaac agacgccgga acagcgtcag
     4621 caaacgctac aacgccagcg tcaggcgcag caattagcgg agcagcaacg cctggctcag
     4681 cagtcgcgta cgactgaaca aagctggcag cagcagacgc gtacgtcgca agccgcgccg
     4741 gtacaggcgc aaccgcgcca gtctaaaccg gcttctactc agcagccgta ccaggatctg
     4801 ctgcaaaccc ctgcgcacac gaccgcgcag tcgaagccgc agcaagctgc gccggttgct
     4861 cgtgccgctg acgcgccaaa accgacggcg gagaaaaaag acgaacgccg ctggatggtg
     4921 cagtgcggtt cgttcagagg cgcggaacag gcagagacgg tacgtgctca gctggcgttc
     4981 gaaggatttg actcgaaaat caccaccaac aatggctgga atcgtgtggt cattggcccg
     5041 gtgaaaggca aagagaacgc agacagcacc ctcaatcggt tgaagatggc gggtcataca
     5101 aactgcattc ggctcgccgc cgggggttga aaccctcaaa atccccccca tctataattg
     5161 cattatgccc cgtacttttg tacggggttt gtactctgta ttcgtaacca aggggtcagc
     5221 tcgtgacaac tatagtaagc gtacgccgta acggccatgt ggtcatcgct ggtgatggtc
     5281 aggccacgtt gggcaatacc gtaatgaaag gcaacgtgaa aaaggtccgc cgtctgtaca
     5341 acgacaaagt catcgcgggc tttgcgggcg gtactgcgga tgcttttacg ctgttcgaac
     5401 tgtttgaacg taaactggaa atgcatcagg gccatctggt caaagccgcc gttgagctgg
     5461 cgaaagactg gcgtaccgat cgcatgctgc gcaaacttga agcactgctg gcagtcgcgg
     5521 atgaaactgc atcgcttatc atcaccggta acggtgacgt ggtgcagcca gaaaacgatc
     5581 ttattgctat cggctccggc ggcccttacg cccaggctgc ggcgcgtgca ctgttagaaa
     5641 atactgaact gagtgctcgt gaaattgctg aaaaggcgtt ggatattgca ggcgacattt
     5701 gcatctatac caaccatttc cacaccatcg aagaattaag ctacaaagcg taaggatctc
     5761 ccatgtctga aatgacccca cgcgaaatcg tcagcgaact ggataagcac atcatcggcc
     5821 aggacaacgc caagcgttct gtggcgattg ctctgcgtaa ccgctggcgt cgcatgcagc
     5881 tcaacgaaga gctgcgccat gaagtgaccc cgaaaaatat cctgatgatc ggcccgaccg
     5941 gtgtcggtaa aactgaaatc gcccgtcgtc tggctaagct ggcgaatgcg ccgttcatca
     6001 aagttgaagc gaccaaattc accgaagtag gctacgtcgg taaggaagtg gattctatta
     6061 ttcgcgatct gaccgatgcc gccgtgaaaa tggtacgcgt ccaggctatc gagaaaaacc
     6121 gttatcgcgc tgaagaactg gcagaagaac gtattctcga cgtgctgatc ccacctgcta
     6181 aaaacaactg gggacagacc gaacagcagc aggaaccgtc cgctgctcgt caggcattcc
     6241 gcaaaaaact gcgtgaaggc cagcttgatg acaaagaaat cgagatcgat cttgccgcag
     6301 caccgatggg cgttgaaatt atggctcctc caggcatgga agagatgacc agccagctgc
     6361 agtccatgtt ccagaacctg ggcggccaga agcaaaaagc gcgtaagctg aaaatcaaag
     6421 acgccatgaa gctgctgatt gaagaagaag cggcgaaact ggtgaacccg gaagagctga
     6481 agcaagacgc tatcgacgct gttgagcagc acgggatcgt gtttatcgac gaaatcgaca
     6541 aaatctgtaa gcgcggcgag tcttccggtc cggatgtttc tcgtgaaggc gttcagcgtg
     6601 acctgctgcc gctggtagaa ggttgcaccg tttccaccaa acacgggatg gtcaaaactg
     6661 accacattct gtttatcgct tctggcgcgt tccagattgc gaaaccgtct gacctgatcc
     6721 cggaactgca aggtcgtctg ccaatccgcg ttgaactgca ggcgctgacc accagcgact
     6781 tcgagcgtat tctgaccgag ccgaatgcct ctatcaccgt gcagtacaaa gcactgatgg
     6841 cgactgaagg cgtaaatatc gagtttaccg actccggtat taagcgcatc gcggaagcgg
     6901 catggcaggt gaacgaatct accgaaaaca tcggtgctcg tcgtttacac actgttctgg
     6961 agcgtttaat ggaagagatt tcctacgacg ccagcgattt aagcggtcaa actatcatca
     7021 ttgacgcaga ttatgtgagc aaacatctgg atgcgttggt ggcagatgaa gatctgagcc
     7081 gttttatcct ataatcgcgt tcaatcattt tcatcattgt ttgatggggc tgaaaggccc
     7141 catttttatt ggcgcgtatt atgactgaac aacaaattag ccgaactcag gcgtggctgg
     7201 aaagtttacg acctaaaacc ctccccctcg cctttgctgc aattatcgtc gggacagcgc
     7261 tggcatggtg gcaaggtcac ttcgatccgc tggtcgccct gctggcactg attaccgccg
     7321 ggctattaca gatcctttct aacctcgcca atgattacgg cgatgcggta aaaggcagcg
     7381 ataaacctga ccgcattggg ccgctacgcg gcatgcaaaa aggggtcatt acccagcaag
     7441 agatgaaacg ggcgctcatt attaccgtcg tgctcatctg tctctccggg ctggcactgg
     7501 ttgcagtggc atgccatacg ctggccgatt ttgtcggttt cctgattctt ggcgggttgt
     7561 cgatcattgc cgctatcacc tacaccgtgg gcaatcgtcc ttatggttat atcggtctgg
     7621 gtgatatttc cgtactggtt ttctttggct ggttgagtgt catggggagc tggtatttac
     7681 aggctcatac attgattccg gcactgatcc ttccggcgac cgcatgcggc ctgctggcaa
     7741 cggcagtact gaatattaat aacctgcgtg atatcaatag cgaccgcgaa aatggcaaaa
     7801 acacgctggt ggtgcgctta ggtgaagtga acgcgcgtcg ttatcatgcc tgcctgctga
     7861 tgggctcact ggtgtgtctg gcgctgttta atctcttttc gctgcatagc ctgtggggct
     7921 ggctgttcct gctggcggca ccattactgg tgaagcaagc ccgttatgtg atgcgggaaa
     7981 tggacccggt ggcgatgcga ccaatgctgg aacgtactgt caagggagcg ttactgacta
     8041 acctgctgtt tattttaggg atattcctaa gccagtgggc agcataactg acaaatatca
     8101 attaacaatt gatgattttg ccaacagccc acatagcgcg atatactgaa aattctcgca
     8161 gcaactgaat gttaagccta tgaaatacga tacttccgag ctttgtgaca tctatcaaga
     8221 agatgttaac gtcgtggaac cgctgttctc caactttggc ggacgggcgt cgtttggcgg
     8281 acaaataatc acggtaaaat gtttcgagga caacgggttg ctgtacgatc tactcgaaca
     8341 gaatggccgt ggtcgtgttc ttgtcgtcga tggcggtggt tctgttcgtc gcgcactggt
     8401 cgatgctgaa ctggcgcgtc tggcagtaca aaatgaatgg gaaggtctgg tcatttacgg
     8461 cgcggtgcgt caggtagatg acctggaaga gttggatatc ggcatccagg cgatggcggc
     8521 aattccggtt ggtgccgctg gcgaaggcat tggcgaaagc gatgtccgcg tcaattttgg
     8581 cggtgtcacc ttcttctccg gcgaccatct ttatgccgac aataccggga ttattctttc
     8641 agaagatccg ctggatattg aatgatagaa aaggcaccgc aaggtgcctt ttttctgcgt
     8701 tacctgttgg cctacacagt aaagaaatta cgcgaaagat gaagcgtaat cagacctctt
     8761 ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc gttctgctgt tctttcagat
     8821 ggttgttctc acgctccagc tcttcgcgct gatgctgggc attttgaact tcctgcgaca
     8881 gtgagttgtt tttttctttc agctcttcga tttccatctg caacagagtg atggtatcaa
     8941 tcgcctgctg tacttttgct tccagtttct caaacacttc taatgacatt gtcatacctc
     9001 tcctgaattg caaggcgttg atggataaaa aatcctcgtc ccgattaccg gtgacgcctt
     9061 aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca tcgctgtcca
     9121 gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat
     9181 ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca
     9241 tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgaa gcacacacat
     9301 tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag
     9361 catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt
     9421 atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg taccgggttg
     9481 ttgattttct tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc gtcttttggt
     9541 cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta cctgaccgca
     9601 ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct gtttgcctgt
     9661 ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc tttctgtgct
     9721 gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca gactcatcac
     9781 attgttcgcg gcagcgttga aagtgttgat ctggctggca ctttctctac ttaccctaat
     9841 cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc tattctgatg
     9901 gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc tttggctccc
     9961 ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt gacaggtttt
    10021 gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctggctggc gggctggggc
    10081 aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct tttcggccct
    10141 atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg ccatttgcct
    10201 tgcgatatct gtgttgtgga agaaaaggaa accacaactc cttcagaaca aaaagcttcg
    10261 ctgtaatatg actacgggac aattaaacat gactgaaaaa aaatatatcg ttgcgctcga
    10321 ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt
    10381 gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt tgggtagaac acgacccaat
    10441 ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag
    10501 ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga
    10561 aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga
    10621 aatctgcgag catttaaaac gtgacggttt agaagattat atccgcagca ataccggtct
    10681 ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg
    10741 ctctcgcgag cgtgcacgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat
    10801 ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct ctcgtaccat
    10861 gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc tggatattcc
    10921 gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta tacggtcaga ctaacattgg
    10981 cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggccgcgct
    11041 gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca ctggctgctt
    11101 tatgctgatg aacactggcg agaaagcggt gaaatcagaa aacggcctgc tgaccaccat
    11161 cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa ggtgcggtgt ttatggcagg
    11221 cgcatccatt cagtggctgc gcgatgagat gaagttgatt aacgacgcct acgattccga
    11281 atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg catttaccgg
    11341 gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc tgactcgtgg
    11401 ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc agacgcgtga
    11461 cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc gcgtggatgg
    11521 tggcgcagta gcaaacaatt tcctgatgca gttccagtcc gatattctcg gcacccgcgt
    11581 tgagcgcccg gaagttcgcg aagtgactgc attgggtgcg gcctatcttg ctggtctggc
    11641 ggtcggtttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg aacgcgagtt
    11701 ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt
    11761 caaacgcgcg atggcgtggg aagaacacga cgagtaatgt aaatgccgga tgaagcgttt
    11821 atgccgcatc cggtagtcct gaaaccgtgc gggggcaacc ccgcacacat caataatccc
    11881 acccttcccc tgtgctacac ttcgcgccat tccttactgc ttagagtttg ctatgagacg
    11941 agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg gcgctggctg gctacaaatg
    12001 gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg gtaaacgcca tgcgtattat
    12061 gctcaaccag gtcaacattg acggcaccat cgtcattggt gaaggtgaaa tcgacgaagc
    12121 accgatgctc tacattggtg aaaaagtcgg tactggtcgc ggcgacgcgg tagatattgc
    12181 tgttgatccg attgaaggca cgcgcatgac ggcgatgggc caggctaacg cgctggcggt
    12241 gctggcagta ggcgataaag gctgcttcct caatgcgccg gatatgtata tggagaagct
    12301 gattgtcggg ccgggagcca aaggcaccat tgatctgaac ctgccgctgg cggataacct
    12361 gcgcaatgta gcggcggcgc tcggcaaacc gttgagcgaa ctgacggtaa cgattctggc
    12421 taaaccacgc cacgatgccg ttatcgctga aatgcagcaa ctcggcgtac gcgtatttgc
    12481 tattccggat ggcgacgttg cggcctcaat tctcacctgt atgccagaca gcgaagttga
    12541 cgtgctgtac ggtattggtg gcgcgccgga aggcgtagtt tctgcggcgg tgatccgcgc
    12601 attagatggc gacatgaacg gtcgtctgct ggcgcgtcat gacgtcaaag gcgacaacga
    12661 agagaatcgt cgcattggcg agcaggagct ggcacgctgc aaagcgatgg gcatcgaagc
    12721 cggtaaagta ttgcgcctgg gcgatatggc gcgcagcgat aacgtcatct tctctgccac
    12781 cggtattacc aaaggcgatc tgctggaagg cattagccgc aaaggcaata tcgcgactac
    12841 cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc cgcattcagt ccatccacta
    12901 tctggatcgc aaagacccgg aaatgcaggt gcacatcctc tgattgattt gatcgattga
    12961 gccttccagt ccttcgggac tggaattttt ttgttcggag aacgaagata aggcaagtta
    13021 atcaaaacag gagaaaaaca tggctgattg ggtaacaggc aaagtcacta aagtgcagaa
    13081 ctggaccgac gccctgttta gtctcaccgt tcacgccccc gtgcttccgt ttaccgccgg
    13141 gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc gtccagcgcg cctactccta
    13201 tgttaactcg cccgataatc ccgatctgga gttttacctg gtcaccgtcc ccgatggcaa
    13261 attaagccca cgattggcgg cactgaaacc aggcgatgaa gtgcaggtgg ttagcgaagc
    13321 agctggcttc tttgttctgg atgaagtacc ggactgcgaa acgctatgga tgctggcaac
    13381 cggtacagcg attggccctt atttatcgat tctgcaacta ggcaaagatt tagatcgctt
    13441 caaaaatctg gtcctggttc acgccgcacg ttatgccgcc gacttaagct atttgccact
    13501 gatgcaagaa ctggaaaaac gctacgaagg gaaactgcgc attcagacgg tggtcagtcg
    13561 ggaaacggca gcggggtcgc tcaccgggcg gataccggcg ttaattgaaa gtggggaact
    13621 ggaaagcgcg attggcctgc cgatgaataa agaaaccagc catgtgatgc tgtgcggcaa
    13681 tccacagatg gtgcgcgata cacaacagtt gctgaaagag acccggcaga tgacgaaaca
    13741 tttacgtcgc cgaccgggcc atatgacagc ggagcattac tggtaagcgg ttacttatcg
    13801 ataaacggca cgatgagcaa atccgcactc atcttattga tcatcccgcg atatgccggc
    13861 atcaaacggt tgataaatga gtgatgatga ccacagacaa ggaggtcgca ctgctctttt
    13921 tgcataattt ccagcagtgt ttccggcatt tctccgcgtt caatacgcag ttttgtcttc
    13981 ggccattgaa tatttttcgt cagtttatac agcttgttat ccgacttatt cttcaacaat
    14041 tgaagaatat cttctgttgc agggaagtag atacccggat acaactcgct taagccatca
    14101 tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg cgagctccag agctttattc
    14161 accagtaagg catcttcttc attcccggaa attgccacgc caatgtgttt ataagccatg
    14221 tttaactcct tctaaagcaa ctccatcaag ctaacgaacg cagtgataac tcaaaaataa
    14281 tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag ctcaacttg
//
LOCUS       DAFATA010000109        14121 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFATA010000109 DAFATA010000000
VERSION     DAFATA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.110"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            141..1496
                     /gene="melA"
                     /locus_tag="LGJ20_003930"
     CDS             141..1496
                     /gene="melA"
                     /locus_tag="LGJ20_003930"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK2387936.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            1599..3020
                     /gene="melB"
                     /locus_tag="LGJ20_003931"
     CDS             1599..3020
                     /gene="melB"
                     /locus_tag="LGJ20_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBK2387937.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(3159..3788)
                     /locus_tag="LGJ20_003932"
     CDS             complement(3159..3788)
                     /locus_tag="LGJ20_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBK2387938.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(3911..5557)
                     /gene="fumB"
                     /locus_tag="LGJ20_003933"
     CDS             complement(3911..5557)
                     /gene="fumB"
                     /locus_tag="LGJ20_003933"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBK2387939.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(5635..6975)
                     /gene="dcuB"
                     /locus_tag="LGJ20_003934"
     CDS             complement(5635..6975)
                     /gene="dcuB"
                     /locus_tag="LGJ20_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBK2387940.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(7546..8265)
                     /gene="dcuR"
                     /locus_tag="LGJ20_003935"
     CDS             complement(7546..8265)
                     /gene="dcuR"
                     /locus_tag="LGJ20_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBK2387941.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(8262..9893)
                     /gene="dcuS"
                     /locus_tag="LGJ20_003936"
     CDS             complement(8262..9893)
                     /gene="dcuS"
                     /locus_tag="LGJ20_003936"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBK2387942.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAMARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            10074..10304
                     /locus_tag="LGJ20_003937"
     CDS             10074..10304
                     /locus_tag="LGJ20_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBK2387943.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            10316..10588
                     /locus_tag="LGJ20_003938"
     CDS             10316..10588
                     /locus_tag="LGJ20_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2387944.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            10815..11111
                     /gene="ghoS"
                     /locus_tag="LGJ20_003939"
     CDS             10815..11111
                     /gene="ghoS"
                     /locus_tag="LGJ20_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBK2387945.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            11139..11312
                     /gene="ghoT"
                     /locus_tag="LGJ20_003940"
     CDS             11139..11312
                     /gene="ghoT"
                     /locus_tag="LGJ20_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBK2387946.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(11431..12948)
                     /gene="lysS"
                     /locus_tag="LGJ20_003941"
     CDS             complement(11431..12948)
                     /gene="lysS"
                     /locus_tag="LGJ20_003941"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK2387947.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(13185..>14121)
                     /gene="dtpC"
                     /locus_tag="LGJ20_003942"
     CDS             complement(13185..>14121)
                     /gene="dtpC"
                     /locus_tag="LGJ20_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBK2387948.1"
                     /translation="GGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         3549 a         3390 c         3501 g         3681 t
ORIGIN      
        1 gtaacttatt accataaact cagatttact gctgcttcac gcaggatctg agtttatggg
       61 aatgctcaac ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc
      121 aagcaagcca ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt
      181 cgacgatttt cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg
      241 cgcatattgc cctgatggac attgatccta cccgcctgga agagtcgcat attgtggtgc
      301 gtaagcttat ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag
      361 aagccttaca ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt
      421 gcacggtgac tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata
      481 cgttggggcc gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt
      541 gcgaggacat gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg
      601 cgatgaatac ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc
      661 attcggtgca gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc
      721 tgcgttaccg ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa
      781 ccgccgacgg cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc
      841 aggcaccgaa gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctacgaaa
      901 tgttcaaaaa gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac
      961 cgtggtttat taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg
     1021 agtacccgaa acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata
     1081 aaaacgcctc ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg
     1141 ctatctggac tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg
     1201 ataacctgcc acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc
     1261 agccgaccaa agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca
     1321 acgtacagac gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg
     1381 ccgcgatgat ggacccgcat actgctgccg tgctgggcat tgatgaaata tatgctcttg
     1441 ttgacgacct gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg
     1501 cgactaaacg ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata
     1561 acagaaggcg ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa
     1621 aactcagtta tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt
     1681 acctcatgta ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt
     1741 ttctggtggc gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg
     1801 ctacgcgatc gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact
     1861 ctgtaatctt atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct
     1921 ttgtttgcgt gacctacatc ctctggggca tgacttacac cattatggat attcccttct
     1981 ggtcgctggt tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc
     2041 cgcgtttttt tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta
     2101 attatgtcgg cggtggcgat cggggatttg gctttcagat gttcactctg gtactaatcg
     2161 ccttttttat tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag
     2221 acaatcaacc gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt
     2281 ataaaaacga tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca
     2341 acattattac cggatttgct atctattatt tctcatatgt tatcggtgat gcggatttgt
     2401 tcccctatta tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac
     2461 gcttagttaa atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt
     2521 taagctgtgg tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg
     2581 tgattgcggg tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca
     2641 tggtggcaga taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg
     2701 cttactccgt gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg
     2761 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
     2821 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
     2881 tctacttccg tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc
     2941 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
     3001 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
     3061 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
     3121 aattcacgcc gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
     3181 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
     3241 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
     3301 tccgctccct ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt
     3361 atcgccagcg ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc
     3421 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc
     3481 agcttgtcat acggattacg gctcaaccct aaccattcct gcacctcaaa accaacgggg
     3541 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaaa gaaaatgagc
     3601 gtatagagca gcggcgttaa cggataacgt ttcgcggtgg caaggagtag cggcacgaca
     3661 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
     3721 taaatcagga ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     3781 cgggtcatgg tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa
     3841 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     3901 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatctgc tggaagaagt
     3961 cgttaccttt gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga
     4021 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     4081 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     4141 cgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     4201 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     4261 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     4321 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     4381 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     4441 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     4501 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     4561 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     4621 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     4681 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     4741 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     4801 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     4861 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
     4921 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     4981 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     5041 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     5101 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     5161 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     5221 tgatcgcggt gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt
     5281 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     5341 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     5401 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     5461 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     5521 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     5581 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     5641 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     5701 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     5761 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     5821 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac
     5881 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     5941 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     6001 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     6061 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     6121 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     6181 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     6241 tgcaccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag
     6301 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     6361 ctcttcgtct ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat
     6421 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     6481 atcaaaggtg acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact
     6541 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
     6601 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     6661 accgcaaaga atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     6721 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     6781 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ctggtggttt
     6841 acctggctga aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc
     6901 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     6961 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     7021 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     7081 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa
     7141 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     7201 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     7261 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatctgat ctacccattt
     7321 gtgggtaaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     7381 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
     7441 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
     7501 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     7561 tttcagcagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
     7621 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtattttcg
     7681 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     7741 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     7801 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
     7861 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     7921 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
     7981 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     8041 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     8101 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     8161 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     8221 ctccgcgacc attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc
     8281 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     8341 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     8401 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     8461 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     8521 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     8581 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg gctttcactg
     8641 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     8701 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg
     8761 gctgttttga gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     8821 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     8881 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     8941 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     9001 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     9061 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     9121 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     9181 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgttgctc aaacagcgtg
     9241 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     9301 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     9361 tcattgattt gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt
     9421 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgtgccag aaaaccgcga
     9481 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     9541 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca
     9601 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     9661 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccatt
     9721 gccagtgcct tgttggccag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc
     9781 agatgcacca ccaatagcac cgagaacagt accgcgctga ccattaagat cactgtggta
     9841 ctcaatttca tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc
     9901 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
     9961 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
    10021 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
    10081 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
    10141 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
    10201 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt tgccactgtg gtgaaagtga
    10261 ttgatacgtg cccgagtggt gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
    10321 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
    10381 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
    10441 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
    10501 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
    10561 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
    10621 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
    10681 agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc
    10741 ctatatgaga atcatcaatc ggggttaata atttttgcgt ccccagagca ttcaatattg
    10801 ataggagtca tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt
    10861 atccatcatc ttattcctca gtgttcttaa ggttaagatc attgatgtat gatatgaatt
    10921 tctcttctat cgtggctgat gaatatggga taccacgaca attgaatgaa aattctttcg
    10981 caataacgac atcgttagcc gcaagtgaaa tcgaagattt aatcaggctt aaatgcttag
    11041 acttaccgga tattgatttt gacctcaaca ttatgacagt tgatgactat ttccgtcagt
    11101 tttacaagta gccaggaagg ataagaagaa cataaaaaat ggcactattc tctaaaatat
    11161 taatttttta tgtgattggt gtgaacatat cctttgtcat tatctggttt atctcacatg
    11221 agaaaacaca tattcgttta cttagtgcat tcctggtcgg aataacctgg ccaatgagtc
    11281 tgcctgtggc attacttttt tctctctttt aggagctgta gttgagtgga cccgctggtt
    11341 atcgtgaaac gaagcgtctc tcctcaaaac tggatttgcg gaacgacgaa cgaagcagtc
    11401 gcctgactgc ttcgttcatt aaagtgaaat ttatttctgt gggcgcatcg ccgggaagag
    11461 aataacgtcg cgaatagtat gactgtttgt aaacagcata atcattcggt cgataccaat
    11521 acccagacca gcggttggcg gcagaccata ttccagcgca gtcacgtaat cttcgtcata
    11581 gaacatggct tcgtcgtcac ctgcagcttt agcgttaacc tgttcctgga aacgttcagc
    11641 ctgatcttct gcgtcgttta attcgctaaa accattaccg atttcacgac caccgatgaa
    11701 gaattcaaaa cggtcggtga tttccgggtt aacatcatta cggcgcgcca gcggtgacac
    11761 ttctgccgga tattccgtaa taaaggttgg ctgaatcaga tgtgcttctg ccacttcatc
    11821 aaagatctcc gtgacaatac gtcccagccc ccagcttttc tctaccgtaa ttccgataga
    11881 ttcagctaat gctttagcag catcaaaatt atccaggtcg gccatatcgg tttctggacg
    11941 atattttttg attgcttcgc gcatggtgag tttttcaaac ggtttgccga aatcaaacac
    12001 atgctcgcca taagtgactt tagtggtacc cagaacctct tgtgccaggg tgcggaacag
    12061 ggactctgtc agttcaatca aatcgtggta atccgcatac gccatgtaga gttccatcat
    12121 tgtgaactca ggattatggc gaacagaaat accttcatta cggaagttac ggttgatttc
    12181 gaatacccgt tcaaaaccgc ctacaaccag acgtttcaga tacagctccg gcgcgatacg
    12241 caggtacata tctaaatcca gagcgttatg atgggtaata aacgggcgag cagatgcccc
    12301 acctggaatt acctgcatca tcggggtttc tacttccata aagccgcgcg cgaccatgaa
    12361 ctggcggata gcggccagaa tttttgaacg gacaacaaac gtttgacggg atttatcgtt
    12421 agcgatgagg tccagataac gttgacgata acggacttcc tgatcctgca gaccatggaa
    12481 tttatctggt aaaggacgta gtgcttttgt cagcaggcgc aactcagtac agtgaatgga
    12541 aagctcaccg gtttgcgtct taaacagcgt accgcgggca ccgataatgt cacccagatc
    12601 ccatttttta aactgatcgt tataaacacc ttctggcagg ctatctcttg caacgtacag
    12661 ttgaatacgg ccaccgacat cctgcaacgt tacaaaggag gctttcccca tgatacgacg
    12721 ggtcatcatt cggccagcaa ccgagacttc aatgtttaag gattccagtt cctggttatc
    12781 cttcgcatca aactcttcgt gcaactggtc agaggtatgg tcgcggcgaa aatcattggg
    12841 aaacgccaca ccttgctgac gtagtgccgc cagtttttcg cggcgatttc tcagttcatc
    12901 gttaaaatca atagcctcat tggctccccg tgtttcttgt tcagacattt tggttcctct
    12961 aaatccagct ttcaatttag cgtagataaa gagacagatc ggtacactgt attatctgcc
    13021 tcgactatta gtaactcagt ttatcttatc gaaactaacg cttacctcaa gttgatatca
    13081 ctaataaata accatcaaaa tggtttaaat gacttattaa tctcgatttg ttagtgttat
    13141 aaactgaaag ttaatttatt cataaccaat tgtttttacg acagttaatc gttgctctcc
    13201 tgtatcatat tcgtcggcgt gctaaacaga aaacgggtag caaaggccaa tacgacgatg
    13261 atcgcgacac aagccaacgt ccactctccc atctgagaaa agaatcgctg gtaagctgca
    13321 attgccatac cgctaatttg cgactctgtc gtctgctgtg ccacgacgcc tgccagccag
    13381 ttggcgaccg cgcctgttgc cagcatataa atcccggtta atacgccaga cattttcaga
    13441 cgcgtgattt gcgcaatcgc caccgggtca ataaagagtt cggcaaagcc cattagcgcc
    13501 agcccggata tcatcacgcc cattgacgct tgaccgtcag ccgctgcatg tcgggcatca
    13561 aatgccaaca acataaagcc acaagccatc agcagtaagc caaaggcaaa cttcagccag
    13621 acgcgcaatg ttgagttgcc gcggctttct ggcgacgcca gccaggccag tacaacccca
    13681 gcgagcatca ccgcaattgc attcaccgac tggaatagtg ctgtaggtac ttcaatattg
    13741 aatgcctgac gattcacaaa gcgatcgata aacaagctga tggtactgcc gccctgttgt
    13801 gccagtaccc agaacaatgt cccgacaaac atcaacaata caatttgcca aagagcacgg
    13861 cgatgttcgg ggaatttgat catcatgcgg gcaatgattt gtgcggcaat gaggcaaacg
    13921 atcgccagca aatatcccga ccagtcgttc tccagcagca gggtaaaaaa tactggggct
    13981 aaacagagca tcaccactaa ccagctccat actggtaagg caaatttcac gctagtgagc
    14041 gcttttttat ccatactacg cgtggactgg aaatgacgat gcccgcttaa gaaaatcaac
    14101 aaaccgataa acatgccgcc a
//
LOCUS       DAFATA010000110        13997 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFATA010000110 DAFATA010000000
VERSION     DAFATA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.111"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1256
                     /gene="glgB"
                     /locus_tag="LGJ20_003943"
     CDS             <1..1256
                     /gene="glgB"
                     /locus_tag="LGJ20_003943"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBK2387949.1"
                     /translation="FGTRDDFRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTN
                     LYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALYWIERFGIDALRVDAVASMIYR
                     DYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQD
                     MGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDEVV
                     HGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDASLDWH
                     LLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKERSVLIFVRRD
                     KEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTDSMHYHGSNAGNGGTVHSDEIA
                     SHGRQHSLSLTLPPLATIWLVREAE"
     gene            1253..3226
                     /gene="glgX"
                     /locus_tag="LGJ20_003944"
     CDS             1253..3226
                     /gene="glgX"
                     /locus_tag="LGJ20_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBK2387950.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            3244..4539
                     /gene="glgC"
                     /locus_tag="LGJ20_003945"
     CDS             3244..4539
                     /gene="glgC"
                     /locus_tag="LGJ20_003945"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBK2387951.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            4539..5972
                     /gene="glgA"
                     /locus_tag="LGJ20_003946"
     CDS             4539..5972
                     /gene="glgA"
                     /locus_tag="LGJ20_003946"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBK2387952.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            5991..8438
                     /gene="glgP"
                     /locus_tag="LGJ20_003947"
     CDS             5991..8438
                     /gene="glgP"
                     /locus_tag="LGJ20_003947"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBK2387953.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            8563..10071
                     /locus_tag="LGJ20_003948"
     CDS             8563..10071
                     /locus_tag="LGJ20_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK2387954.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            10086..10796
                     /locus_tag="LGJ20_003949"
     CDS             10086..10796
                     /locus_tag="LGJ20_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387955.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVLKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            10799..11566
                     /locus_tag="LGJ20_003950"
     CDS             10799..11566
                     /locus_tag="LGJ20_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387956.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            11569..12174
                     /locus_tag="LGJ20_003951"
     CDS             11569..12174
                     /locus_tag="LGJ20_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387957.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGNWVTCLSLAILAQGPLFNYLQ"
     gene            complement(12226..13731)
                     /gene="glpD"
                     /locus_tag="LGJ20_003952"
     CDS             complement(12226..13731)
                     /gene="glpD"
                     /locus_tag="LGJ20_003952"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBK2387958.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYIWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            13921..>13997
                     /gene="glpE"
                     /locus_tag="LGJ20_003953"
     CDS             13921..>13997
                     /gene="glpE"
                     /locus_tag="LGJ20_003953"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="belongs to rhodanese family; thiosulfate
                     thiotransferase; in Escherichia coli this enzyme catalyzes
                     the formation of thiocyanate from thiosulfate and cyanide
                     with low efficiency; contains an active site cysteine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBK2387959.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVD"
BASE COUNT         3303 a         3425 c         3726 g         3543 t
ORIGIN      
        1 gttttggtac tcgcgacgac ttccgttatt tcattgatgc cgcacacgca gctggtctga
       61 acgtgattct cgactgggtg ccaggccact tcccgactga tgactttgcg cttgccgaat
      121 ttgatggcac gaacttgtat gaacacagcg atccgcgtga aggctatcat caggactgga
      181 acacgctgat ctacaactat ggtcgccgtg aagtcagtaa cttcctcgtc ggtaacgcgc
      241 tttactggat tgaacgtttt ggtattgatg cgctgcgcgt cgatgcggtg gcgtcaatga
      301 tttatcgcga ctacagccgt aaagaggggg agtggatccc gaacgaattt ggcgggcgcg
      361 agaatcttga agcgattgaa ttcttgcgta ataccaaccg tattcttggt gagcaggttt
      421 ccggtgcggt gacaatggct gaggagtcta ccgatttccc tggcgtttct cgtccgcagg
      481 atatgggcgg tctgggcttc tggtacaagt ggaacctcgg ctggatgcat gacaccctgg
      541 actacatgaa gctcgacccg gtttatcgtc agtatcatca cgataaactg accttcggga
      601 ttctctacaa ctacactgaa aacttcgtcc tgccgttgtc gcatgatgaa gtggtccacg
      661 gtaaaaaatc gattctcgac cgcatgccgg gcgacgcatg gcagaaattc gcgaacctgc
      721 gcgcctacta tggctggatg tgggcattcc cgggcaagaa actactgttc atgggtaacg
      781 aatttgccca gggccgcgag tggaaccatg acgccagcct cgactggcat ctgttggaag
      841 gcggcgataa ctggcaccac ggtgtccagc gtctggtgcg cgatctgaac ctcacctacc
      901 gccaccataa agcaatgcat gaactggatt ttgacccgta cggctttgaa tggctggtgg
      961 tggatgacaa agaacgctcg gtgctgatct ttgtgcgtcg cgataaagag ggtaacgaaa
     1021 tcatcgttgc cagtaacttt acgccggtgc cgcgtcatga ttatcgcttc ggcataaacc
     1081 agccgggcaa atggcgtgaa atcctcaata ccgattccat gcactatcac ggcagtaatg
     1141 caggcaatgg cggcacggta cacagcgatg agattgccag ccacggtcgt cagcattcac
     1201 taagcctgac gctaccaccg ctggccacta tctggctggt tcgggaggca gaatgacaca
     1261 actcgccatt ggcaaacccg ctcccctcgg cgcgcattac gacggtcagg gcgtcaactt
     1321 cacacttttc tccgctcatg ccgagcgggt agaactgtgt gtctttgacg ccaatggcca
     1381 ggaacatcgc tatgacttgc cagggcacag tggcgacatt tggcacggtt atctgccgga
     1441 tgcgcgcccg ggtttgcgtt atggttatcg cgttcatggc ccctggcaac ccgccgaggg
     1501 gcatcgcttt aacccggcga agttgttgat tgatccttgc gcgcggcaaa ttgaggggga
     1561 gtttaaagat aacccgctgc tgcacgccgg tcataatgaa cctgactatc gcgacaacgc
     1621 cgccattgcg ccgaaatgcg tagtggtggt tgatcactat gactgggaag atgatgcccc
     1681 gccgcgcacg ccgtggggca gcaccatcat ttatgaagcc catgtcaaag gattaacgta
     1741 cttgcacccg gagatcccgg tcgagatccg tggcacttat aaagccctcg ggcatccggt
     1801 gatgatcaac tatttgaaac aattgggcat taccgcgctg gaactgctgc cagtggcgca
     1861 gtttgccagt gaaccacgtc tgcaacgcat ggggctaagt aactactggg gttacaaccc
     1921 ggtggcgatg tttgcgctgc atccggcgta tgcctgctcg ccagaaacgg cgctggatga
     1981 gtttcgcgat gcaatcaaag cactgcataa agcgggtatc gaagtcattc ttgatatcgt
     2041 gctcaaccat agtgcggaac tggacctcga cggcccgtta ttctcgctgc gtgggatcga
     2101 taaccgtagc tattattgga taagagaaga cggcgattat cacaactgga ccggttgcgg
     2161 caacacgctc aatttgagtc atccggcggt ggtggattat gccagcgcct gcctgcgtta
     2221 ttgggtagaa acctgccacg tcgatggttt ccgctttgat ctggcggcag tcatgggccg
     2281 tacgccagag ttccgtcagg atgcgccgtt gtttaccgct atccagaact gcccggtgct
     2341 ctcgcaggtg aagttaattg ctgaaccgtg ggatatcgct cctggtggtt atcaggtggg
     2401 aaatttcccg ccgctgtttg ccgagtggaa cgatcatttc cgcgatgctg cccgtcgttt
     2461 ctggctacat tatgatttgc ctctgggggc gtttgccggg cgttttgctg cctccagcga
     2521 tgtttttaaa cgtaatggtc gtctgccgag tgccgcgatt aatctcgtca ccgcgcatga
     2581 cggttttacg cttcgcgact gcgtttgctt caaccataaa cacaatgaag caaacggaga
     2641 agaaaatcgc gacgggacca acaacaatta cagtaacaat catggtaaag aagggttagg
     2701 cggttctctt gacctggttg aacggcggcg cgacagcatt cacgccctgt taacaacgtt
     2761 gttgctctcc cagggtacgc cgatgttact ggccggtgac gaacatggtc acagccagca
     2821 tggcaataac aatgcctact gtcaggataa ccaattaacc tggttggact ggtcgcaggc
     2881 aagcagtggt ttaaccgcat ttaccgccgc gttaatccat ctgcgcaagc gcattcccgc
     2941 tttggtggag aatcgctggt gggaagaagg cgacggcaat gtccgttggc taaatcgata
     3001 tgctcaacct ttaagcacgg atgagtggca aaacgggccg aaacagctgc aaattctgct
     3061 ctcggatcgc tttttgatcg caattaacgc cacgcttgag gtaacagaga ttgttttacc
     3121 tgctggggag tggcacgcca ttcccccatt cgctggagag gataacccag tgattacggc
     3181 tgtctggcag ggacctgcac acggattgtg tgtgttccag agatgataaa aaaggagtta
     3241 gtcatggtta gtttagagaa gaacgatcac ttaatgttgg cgcgccagct gccattgaaa
     3301 tctgttgccc tgatactggc gggaggacgt ggtacccgcc tgaaggattt aaccaataag
     3361 cgagcaaaac cggccgtaca cttcggcggt aagttccgca ttatcgactt tgcgctgtct
     3421 aactgcatca actccgggat ccgtcgtatg ggcgtgatca cccagtacca gtcccacact
     3481 ctggtgcagc acattcagcg cggctggtca ttcttcaatg aagaaatgaa cgagtttgtc
     3541 gatctgctgc cagcacagca gagaatgaaa ggggaaaact ggtatcgcgg caccgcagat
     3601 gcggtcaccc aaaacctcga cattatccgc cgttataaag cggaatacgt ggtgatcctg
     3661 gcgggcgacc atatctacaa gcaagactac tcgcgtatgc ttatcgatca cgtcgaaaaa
     3721 ggcgcacgtt gcaccgttgc ttgtatgcca gtaccgattg aagaagcctc cgcatttggc
     3781 gttatggcgg ttgatgagaa cgataaaatt atcgaattcg ttgaaaaacc tgctaaccca
     3841 ccgtcaatgc cgaacgatcc gagcaaatct ctggcgagta tgggtatcta cgtctttgac
     3901 gccgactatc tgtatgaact gctggaagaa gacgatcgtg atgagaactc cagccacgac
     3961 tttggcaaag atttgattcc caagatcacc gaagccggtc tggcctatgc gcacccgttc
     4021 ccgctctctt gcgtacaatc cgacccggat gccgagccgt actggcgcga tgtgggtacg
     4081 ctggaagctt actggaaagc gaacctcgat ctggcctctg tggtgccgga actggatatg
     4141 tacgatcgca attggccaat tcgcacctac aatgaatcat taccgccagc gaaattcgtg
     4201 caggatcgct ccggtagcca cgggatgacc cttaactcac tggtatccgg cggctgtgtg
     4261 atctccggtt cggtggtggt gcagtccgtt ctgttctcgc gcgttcgcgt gaattcattc
     4321 tgcaacattg attccgccgt attgttaccg gaagtatggg taggtcgctc gtgccgtctg
     4381 cgccgctgcg tcatcgatcg tgcttgtgtt attccggaag gcatggtgat tggtgaaaac
     4441 gcagaggaag atgcacgtcg tttctatcgt tcagaagaag gcatcgtgct ggtaacgcgc
     4501 gaaatgctac ggaagttagg gcataaacag gagcgataat gcaggtttta catgtatgtt
     4561 cagagatgtt cccgctgctt aaaaccggcg gtctggctga tgttattggg gcattacccg
     4621 cagcacaaat cgcagacggc gttgacgctc gcgtactgtt gcctgcattt cccgatattc
     4681 gccgtggcgt taccgatgcg caggtagtat cccgtcgtga taccttcgcc ggacatatca
     4741 cgctgttgtt cggtcattac aacggggttg gcatatacct gattgacgcg ccgcatctct
     4801 atgatcgtcc gggaagcccg tatcacgata ccaacttatt tgcctatacc gacaacgtat
     4861 tgcgttttgc gctgctgggg tgggttgggg cagaaatggc cagcgggctt gacccattct
     4921 ggcgtcctga tgtggtgcat gcgcacgact ggcatgcagg ccttgcgcct gcgtatctgg
     4981 cggcgcgcgg gcgtccggcg aagtcggtgt ttactgtgca caacctggcc tatcaaggca
     5041 tgttttatgc acatcatatg aatgacatcc aattgccatg gtcattcttt aatattcatg
     5101 ggctggaatt caacggacaa atctctttcc tgaaggccgg tctgtactat gccgatcaca
     5161 ttacggcggt cagtccaacc tacgctcgcg agatcaccga accgcagttt gcctacggta
     5221 tggaaggtct gttgcaacag cgtcaccgtg aagggcgtct ttccggcgta ctgaacggcg
     5281 tggacgagaa aatctggagt ccagagacgg acttactgtt ggcctcgcgt tacacccgcg
     5341 atacgttgga agataaagcg gaaaataagc gccagttaca aatcgcaatg gggcttaagg
     5401 ttgacgataa agtgccgctt tttgcagtgg tgagccgtct gaccagccag aaaggtctcg
     5461 acctggtgct ggaagcctta ccgggtcttc tggagcaggg cgggcagctg gcgctactcg
     5521 gcgcgggcga tccggtgctg caggaaggtt tccttgcggc ggcagcggaa taccccggcc
     5581 aggtgggcgt tcagattggc tatcacgaag cattttcgca tcgcattatg ggcggcgcgg
     5641 acgtcattct ggtgcccagc cgttttgaac cgtgcggctt aacgcaactt tatggattga
     5701 agtacggtac gctgccgtta gtgcggcgca ccggtgggct tgctgatacg gtttctgact
     5761 gttctcttga gaaccttgca gatggcgtcg ccagtgggtt tgtctttgaa gatagtaatg
     5821 cctggtcgct gttacgggct attcggcgtg cttttgtact gtggtcccgt ccttcactgt
     5881 ggcggtttgt gcaacgtcag gctatggcaa tggattttag ctggcaggtc gcggcgaagt
     5941 cgtaccgtga gctttactat cgcttgaaat agttttcagg aaacgcctat atgaatgctc
     6001 cgtttacata ttcatcgccc acgcttagcg tagaagctct taagcactct atcgcttaca
     6061 agctgatgtt tacgattgga aaggacccgg tcgtcgccaa taaacatgaa tggctgaacg
     6121 caacgttatt tgctgtgcgc gatcgtctcg tggagcgctg gttacgttca aaccgtgccc
     6181 agttgtcgca agaaactcgt caggtttact acctgtcgat ggagtttttg attggccgta
     6241 cgctctccaa cgccatgttg tcgctaggaa tttacgaaga tgtacagggc gcactggaag
     6301 cgatggggtt aaatctcgaa gagctgattg atgaagaaaa tgatccgggc ctcggtaacg
     6361 gtggcctggg acgtctggcg gcttgcttcc ttgattctct ggcgacgtta gggttgccgg
     6421 ggcgcggtta cggcatccgc tatgactacg gtatgttcaa gcagaacatc gttaacggta
     6481 gccagaaaga gtcgccagac tactggctgg aatacggtaa cccgtgggaa ttcaaacgcc
     6541 acaacacgcg ctataaagtc cgttttggtg gtcgcattca gcaggaaggt aaaaaaacgc
     6601 gctggattga aaccgaagag attctgggag tcgcttacga tcagataatc cctggttacg
     6661 ataccgacgc gaccaacacg ttgcgtttgt ggagtgcgca agccagtagc gaaattaacc
     6721 tcggtaaatt caaccagggt gactacttcg cggcagtgga agataaaaac cactccgaga
     6781 acgtatctcg cgtactgtat ccggatgact ccacctactc cgggcgtgag ctgcgcctgc
     6841 gtcaggaata cttcctggtt tcctcgacca ttcaggacat tttaagccgc cattatcagt
     6901 tgcataaaac ctacgataac ctggcggata aaatcgcgat tcatctcaat gatacccatc
     6961 cggtactgtc gattcctgag atgatgcgtc tgctgatcga tgagcaccaa tttagctggg
     7021 acgacgcgtt tgaggtgtgt tgtcaggtct tctcctacac taaccacacg ctgatgagcg
     7081 aggcgctgga aacctggccg gttgatatgc tgggtaaaat tctgccgcgt cacctgcaga
     7141 tcatctttga aatcaacgac tatttcctga aaaccttgca ggaacagtat ccgaacgata
     7201 ccgatctgct gggacgggcg tcgatcattg atgaatccaa cggtcgtcgt gtgcgtatgg
     7261 cctggctggc ggttgttgtg agccacaaag ttaacggtgt atcggaactg cactctaatc
     7321 tgatggtgca atcgttgttt gccgactttg cgaaaatctt cccgggtcgt ttcaccaacg
     7381 tcaccaacgg tgtgacgccg cgtcgctggc tagcggtagc gaacccatcg ctttcagccg
     7441 tgctggacga acacctgggc cgtaactggc gcaccgacct tagcctgctt aatgagctgc
     7501 aacaacactg tgatttccca atggttaatc acgctgtgca tcaggcgaag ctggagaaca
     7561 aaaagcgtct ggcagagtat atcgcccagc agctgaatgt ggtggtgaat ccaaaggcgt
     7621 tgttcgatgt acaaatcaaa cgtattcacg aatacaaacg tcaattgatg aatgtgttgc
     7681 atgtgattac ccgctataac cgcatcaagg ccgacccgga tgcgaagtgg gtaccgcgcg
     7741 tgaatatttt tggcggtaag gcggcttcgg cctattacat ggcgaagcac attattcatt
     7801 tgatcaatga cgtagcgaaa gtgatcaaca acgatccgca gattggcgac aagctgaaag
     7861 tcgtgttcat cccgaactac agcgttagcc tggcgcagtt gatcattccg gcggccgatc
     7921 tgtctgaaca gatttcgctg gcggggacgg aagcttccgg caccagtaac atgaagtttg
     7981 cgcttaacgg tgcgctgact atcggtacgt tggacggtgc gaatgtcgag atgctggatc
     8041 atgtcggtgc tgacaatatc tttatctttg gtaacactgc ggaagaagtg gaagaactgc
     8101 gtcgtcaggg ctacaaaccg cgtgaatact acgagaaaga tgaggagctg catcaggtgc
     8161 tgacgcaaat cggcagcggt gtattcagtc cggaagatcc gggtcgctat cgcgatctgg
     8221 ttgattcgct gatcaacttc ggcgatcact atcaggttct ggcggattat cgcagctatg
     8281 tcgattgtca ggataaagtc gacgaactct acgagcttca ggaagagtgg accgcaaaag
     8341 cgatgctgaa cattgccaat atgggctact tctcttctga ccgtactatc aaagagtacg
     8401 ccgatcatat ctggcatatt gatccggtga gattgtaatt tcactaataa atagaacggg
     8461 gccaaagggt cccgtttttt tcgccatcat ttcggaaaag tgtccagaag gggcaagggg
     8521 actcggggag tataatttcg ccattcagaa agaatggaaa gaataatgca aaacagaaaa
     8581 tggattttga cctcgctggt aatgactttt ttcggcatcc ccattctggc gcagtttttg
     8641 gcggcggtta ttgccatgct gggtgtcgga cttgccggta ttattgaagt ttgtaatatc
     8701 tttatcacgc caacaattta ccttctgctc aacattttta tgctggcgct gggcgcatta
     8761 atgctatttt tctcggggcg agtgtgggcg gacgatagtg caccagaaaa aagagaaata
     8821 gccgtctggc gacaatgtct ttttttagta cccgcattat taaccctggg ggtctggata
     8881 atcgcgctgc atctggcaga ttatcaattt cgccagatgg gagcgggttg gttggctgat
     8941 cttatgctcc cctggctggg cgttttgtta gcctcattag tcggtggtga gtactggtgg
     9001 ttagtcatta tacctgttgg cgcgcatatc agtttttcgc tggggtacgg ctggccgacc
     9061 agatatcctt taacgggcac gtccgggtta cgttgccgta attctctctt gtttatcctt
     9121 ctcatgcttg gttttgtcgc cggttaccag gcttatttat ataaacagct taatcccggc
     9181 gtcggtgtgc gtgaaaatat tgatacctgg gcctggcgac ccgataaact caataatcaa
     9241 ctgacaccac tgcgtggtaa accgcaaatt cagttcacgc aaaactggcc gcgacttgat
     9301 ggcgcaacgg cggcgtaccc catttatgcc tctgcctttt atgcactaag cgttttgccg
     9361 gaagattttc acgaatggga atatctggcg aactctcgta ctcccgaagc atataacaag
     9421 attgttaaag gtaatgccga tattatcttt gtggctcaac cttccggtgg gcagaaaaaa
     9481 cgcgcggagg aatcgggcgt cactttgatt tacacgcctt ttgcccgtga agcgtttgtt
     9541 ttcatcgtca atgtagataa cccggttaat tccctgaccg aacaacaagt gcgtgacatc
     9601 ttcagtggtg caattaccaa ctggcgcacg gttggcggta acgatcagga gatccagacc
     9661 tggcaacgcc cggaagactc tggtagccag acggtgatgc aatcccaggt catgaaaaat
     9721 gtccgcatga tctcgccgca ggaaacagaa gtggcaagta tgatggaggg aatgattaaa
     9781 gtcgttgccg aataccgtaa tacaaacaac gcaataggtt acaccttccg ttattacgca
     9841 acacaaatga acgccgataa aaatataaaa ctgctagcga ttaacggtat tgcaccgact
     9901 gccgaaaaca ttcgcaacgg caaatatccc tatatcgtcg atgcctttat ggtaacgcgg
     9961 gaaaatatga cgtcagaaac acaaaaattg gtggagtggt ttttaacgcc gcaggggcag
    10021 agcctggtgg aagatgtagg ttatgtgccg atgtataaaa cgttgcatta aggcggataa
    10081 taaaaatgga attagttaat acattatttg caagccttgt cggtactgat ccattcactg
    10141 gcgtggatat tacgattgcc aactgcaaat cggcatactg ggatgaaggc attgttcagc
    10201 aactcattaa ccaggccctg gatgaagggg aaaagttcgt cggtgctgac ggtcttgagg
    10261 gattgcttcg atataatgtc acattaaata ttggcttaac ctccagcaag gtatggccgg
    10321 gattttccct cgataccgcc accattagcc gtctttgtgc ctgtggtgct gattttggat
    10381 ttgaccccta tatcagtgat gtaccagatg ttcaatgtga cctgaacacg acaaatgacg
    10441 tcaccgtaca atttacggcg atgctaaatc cggatgagcg ggtgataata gcaaagcgcc
    10501 cgcttaaaaa atgtgactcc tggattgagg atgtctatat ctttcaggtc ttaaaagatg
    10561 cctggaaatt tcataacaat aacagtttac gcggatttcg cgataagcag gccgaactga
    10621 aactctacac gcgtcattac tcagtggaaa attgcgcaga ggaaagctgt cgagactgca
    10681 atagttgcat tcgccccagc ttttcccttt cgcgtagcgc ccttattcgc ctgaacgcgg
    10741 cgaatgctcg ttttgtctat caaccgttta cacgcgatca gcgggcgagg gggtaattat
    10801 gcaacccagc cccgccatgc aggcattaat tgagcagatt tatcatattt ttcgtcgtta
    10861 tccggtgccg cagaaatttg tggtttgttg tgaatattgc ctgagtcagc aggaacaaaa
    10921 ggtattacgg agtacatcgt tacgagcgat ctcgtattcc ttaatcaatg catggaatag
    10981 tagcccaggg cctgatccgc aaaatagcga tgaggtccgc tattttctgc cgcgtttact
    11041 ggaatttgtt gcacaggggc agtttgacaa tattcacgaa gtattttctc tacggcgtat
    11101 aaaccttgcc agtaaagaaa attggcgtga ggatgaatgg aaaattttgc aacgatttgc
    11161 ctgccagtat atgactgatt gggtatccgg cgatgaagct gtcgaattac agtatatgct
    11221 ggaaatgttt ttccgtgccg atatcgctct tgcgccattg ctggatgcca ttaattcagt
    11281 tcccggcttt tggtcgaccg tttctcttgc ctgtttattg aacagatatt gtgaggatta
    11341 tatcagagat aaccaggacg atatcgataa cgtcattacc acccaaatca atgcctgggc
    11401 tagaaataat catcctttac tgaaagaaag agcgcggcaa gcgattgaga acccgctaaa
    11461 acagcccgaa ccaatgacag aatatcaggt ctgggaagat gactggataa ttgatgagtg
    11521 tttatgcgcg atgtatgacg catcatccga atcaccagga aaataaaaat gattaaattc
    11581 cggctttata ttccccctgt aattctcggt tttgttatcg tgccattatt ggtatggccg
    11641 acggttattg tcttagccgt acttatattc acgttaactt ttctggcaga aataatattc
    11701 tcctttccgc tcctggttgt gcgtatttct cttcaggaat tacaacttga gttaatggtt
    11761 gaatatgcac tttttttcag tgtaatggct ggcatcggtt ggcaattctc ccgcagaacg
    11821 cctcctgaat taaaaaacag actgcattgc tggctggtct tttcgccggt ctatttctgg
    11881 ttaattctct cgaatttcat tctttatatt tctccagaga aatcagcgtt gctggaaaat
    11941 atccgaaatt tctttctgac gtttgtctgg cctcccctga atttttcccc tttttggccg
    12001 cagccttgga ctgattttgt cggcccgatt agtgcacagc ttggttttgc attgggatat
    12061 tattgccatt ggtgcagaaa aaatagaaac cagaggaaga agtggggcaa ttgggtaacg
    12121 tgcttaagtt tggcgatttt agctcagggg ccgttattca attatttaca atgaatagtc
    12181 taaggaatcc aaatagcccg gttgcacttc aaccgggcat catccttacg acgccagcga
    12241 caacttctgc tgcgtatatt ccaccagcca ctgactcaca cgagattgtt gatccgcatt
    12301 cagccacatg ccttgttttg tgcgacgcca aagggcatcg tcggtgcggc ggacccattc
    12361 gtgatccacc aggtatttca gttccgcttc gtagaactca tgaccgaaat cctccccgag
    12421 gtcgcttatc gcccccgcat tgccgagcag cagctcgctg ttgctgccgt aagtgcgtgc
    12481 gtaatgacgc gccagcgatt cagtcaggaa cgggtaacgg cggcgcaggc gagcggcgta
    12541 atcgtcgcgg tcgccttcaa tggcaccacc cggcagcacg ctctctttcg tccacgccgg
    12601 gccaatgccc tgataatacg gcgtcagttt ttccagcgca tgttccgcca gtttgcggta
    12661 ggtggtcagc ttaccgccga ataccgacag cagcggtgct ttgccgtttt catcatggat
    12721 atcaagggtg taatcacggg taatggcttg cggcgagtcg gactcatcat cacacagcgg
    12781 acgcacaccg gagtaggtcc agacgatatc gtcacggctt agctgctttt taaagtgcgc
    12841 gttatacact ttcagcaggt agttgatttc actctcttca atcttcaccg cttttggatc
    12901 gcctttgtat tccacatcgg tggtgccgat gatggaaaac tcgtccatcc acgggatcac
    12961 gaacacaatg cgtttatctt cgttttgcag aatgtaggcc tgcttttggg tatgcacgcg
    13021 cggcaccaca atatggctgc ctttgatcag gcgaatgcca taaggcgaag gcagatgcat
    13081 cccgtcgtcg aagaactgtt tcacccacgg gccggtggcg ttaaccaggc cgcgcgcttg
    13141 ccagatatat tttttgccgg tatcgatatc ttccgcttcc acaatccaca ggccgttttc
    13201 gcggcgagca gaggtggcgc gagtccgagt aagcacttcg ccgcctttac gcaccaccat
    13261 ctgggcgttg gcgagtacca gacgggcgtc gtctacccaa cagtcagaat attcgaatcc
    13321 gcgcttaatt tccggtttta acactgagtt tgcgccaaaa cgcaaaccgg ttgatcccgg
    13381 caggctggtg cgtttaccca gatgatcgta cataaacaga ccaatccgaa tcatccacgc
    13441 cgggcgcaga tgcggacgat gtggcaggcg aaaacgcatc gggaaggcga tatgcggagc
    13501 cattttcagc agcacttcac gttcagccag cgcctcgctg accaggcgga attcatagtg
    13561 ctcaaggtag cgcaggccac cgtgaatgag ttttgaactg gcggaagagg tcgcgcaagc
    13621 gagatcctgc gcctccagca tcagcacgga taaaccgcgt ccagcggcgt ctgccgcgat
    13681 accagcacca ttgatgccgc cacctatcac aatcagatct ttggtttcca tgctgccctc
    13741 attcactttc gttaaagctc ataaatgttc gttatcgaac atattagcaa agaatcgcgc
    13801 tttaggtaac attgaaaaaa cattttagag tgatatgtat aacattatgg cgtttatctg
    13861 ccgcttcgac gtaaactgtg cggtaaattt gcccacttgt ttgtaaagaa agagagacgc
    13921 atggatcagt tcgaatgtat taacgttgcc gacgcgcacc agaagttgca ggaaaaagag
    13981 gcggtgctgg tcgatat
//
LOCUS       DAFATA010000111        13893 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFATA010000111 DAFATA010000000
VERSION     DAFATA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.112"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1167
                     /locus_tag="LGJ20_003954"
     CDS             <1..1167
                     /locus_tag="LGJ20_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2387960.1"
                     /translation="DHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRIEGTQF
                     VLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLSTAH
                     NAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIFAETGE
                     LYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNIHI
                     PALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQIL
                     RDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAITSIGQ
                     SLNLAVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLKI"
     gene            1425..2006
                     /gene="ddpX"
                     /locus_tag="LGJ20_003955"
     CDS             1425..2006
                     /gene="ddpX"
                     /locus_tag="LGJ20_003955"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBK2387961.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            2020..3570
                     /locus_tag="LGJ20_003956"
     CDS             2020..3570
                     /locus_tag="LGJ20_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2387962.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            3572..4594
                     /locus_tag="LGJ20_003957"
     CDS             3572..4594
                     /locus_tag="LGJ20_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2387963.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            4591..5487
                     /locus_tag="LGJ20_003958"
     CDS             4591..5487
                     /locus_tag="LGJ20_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBK2387964.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            5484..6470
                     /locus_tag="LGJ20_003959"
     CDS             5484..6470
                     /locus_tag="LGJ20_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2387965.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            6463..7335
                     /locus_tag="LGJ20_003960"
     CDS             6463..7335
                     /locus_tag="LGJ20_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2387966.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVR"
     gene            complement(7445..7876)
                     /gene="osmC"
                     /locus_tag="LGJ20_003961"
     CDS             complement(7445..7876)
                     /gene="osmC"
                     /locus_tag="LGJ20_003961"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBK2387967.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            8222..8437
                     /gene="bdm"
                     /locus_tag="LGJ20_003962"
     CDS             8222..8437
                     /gene="bdm"
                     /locus_tag="LGJ20_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBK2387968.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            8539..8676
                     /gene="sra"
                     /locus_tag="LGJ20_003963"
     CDS             8539..8676
                     /gene="sra"
                     /locus_tag="LGJ20_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBK2387969.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            8833..10530
                     /gene="maeA"
                     /locus_tag="LGJ20_003964"
     CDS             8833..10530
                     /gene="maeA"
                     /locus_tag="LGJ20_003964"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBK2387970.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            10664..11674
                     /gene="adhP"
                     /locus_tag="LGJ20_003965"
     CDS             10664..11674
                     /gene="adhP"
                     /locus_tag="LGJ20_003965"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBK2387971.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            11821..12105
                     /locus_tag="LGJ20_003966"
     CDS             11821..12105
                     /locus_tag="LGJ20_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBK2387972.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(12156..12809)
                     /gene="fdnI"
                     /locus_tag="LGJ20_003967"
     CDS             complement(12156..12809)
                     /gene="fdnI"
                     /locus_tag="LGJ20_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBK2387973.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(12802..13686)
                     /gene="fdnH"
                     /locus_tag="LGJ20_003968"
     CDS             complement(12802..13686)
                     /gene="fdnH"
                     /locus_tag="LGJ20_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBK2387974.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(13699..>13893)
                     /locus_tag="LGJ20_003969"
     CDS             complement(13699..>13893)
                     /locus_tag="LGJ20_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2387975.1"
                     /translation="VVTRRLKPLNVNGQQVETVGIPIHWGFEGVARKGYIANTLTPNV
                     GDANSQTPEYKAFLVNIEKA"
BASE COUNT         3388 a         3558 c         3584 g         3363 t
ORIGIN      
        1 gaccatattc aggatgtgat tgatagcctt ggctatgcgt gggccgatca ggcattgctg
       61 gaagtggtca atcgctttcg tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa
      121 ggtacgcagt ttgtcctcgt gagcctggaa aacgacgtca gtaacattac ccagatcgcc
      181 gatgagctac ggaatgtggt cagcaagccg ataatgattg acgataaacc attcccgctt
      241 accttgagta ttggcatcag ctacgacgtg ggtaaaaacc gcgattacct actctccact
      301 gctcacaatg caatggatta tattcgcaag aatggcggta acggctggca gttcttcagc
      361 ccggcgatga acgaaatggt aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg
      421 attagcaata accaactgaa acttgtttac cagccgcaaa tcttcgcaga aacgggtgaa
      481 ctgtacggca tcgaagccct tgcgcgctgg cacgatcccc agcatggtca tgtgccccct
      541 tcacggttta ttcctctcgc agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc
      601 atcgcggaag cttgccgtca gttagcagaa tggcgtagcc agaatattca tatcccggcg
      661 ctttccgtta acttgtcggc actgcacttt cgcagtaatc aactgcctaa tcaggtgtct
      721 gatgcaatgc acgcctgggg tattgacggc caccagctga cggtagaaat cacggaaagc
      781 atgatgatgg aacacgatac cgaaatcttt aagcgcattc agatcctgcg tgatatgggc
      841 gtgggcttat cggtagatga ttttggtacg ggcttttccg gattatcccg cttagtcagt
      901 cttccggtaa cggaaatcaa aattgacaaa agttttgtcg atcgttgtct gacggaaaaa
      961 cgcatcctgg ccttacttga agccattacc agcattgggc aaagcctcaa tttagccgtc
     1021 gtggcggaag gggtcgaaac caaagagcaa tttgagatgc tacgtaagat ccactgtcgc
     1081 gttattcagg gatatttctt ttcccgcccc cttcccgccg aagaaattcc aggctggatg
     1141 agcagcgtgt taccgctgaa aatctgacaa attcctctcg cccgcactcg cgggttttcc
     1201 tttaaggtga cactgtcact taaatactgt gatttcagct acgattccgg gagattcctt
     1261 ccttaacttc ccctcttgca ccaaaactgc actacgctgc acaaatgttg atcaaaagtt
     1321 aaaaaatata actttaagcc agcattctgg cgcttctccc ggcatggcat gagatctgca
     1381 taagcggaaa gcgcagcaat ttttgtctta tacaggaacc gtttatgtcg gataccaccg
     1441 aactggttga tttagccgta atcttccctg atctggagat cgaattgaaa tacgcctgcg
     1501 ctgataacat cacaggtaaa gctatttatc agcaagcgcg ttgtctgtta cacaaggatg
     1561 cgattaccgc gctggcgaaa agtatcagca tcgcccagct gtcaggctta caactggtga
     1621 tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc
     1681 cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc
     1741 tgacgcttcg tgatgaacac gggaacatcc tcgatatggg cgcagggttc gatgaaatgc
     1801 acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcggctgt
     1861 tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact
     1921 tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattcacc tgttttatat
     1981 cccctggcac acaacacgtc agttaatcag gaaactgtca tgaagagatc gataccgttt
     2041 cgtcccacat tgctcgcgct cgtccttgcc acaaatttcc cggttgcgca cgccgccgta
     2101 ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta
     2161 acaatagata ataacgactg gacggtgacc tacccgtctt atcagcggct ggttcagtac
     2221 aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa
     2281 gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa atttgccgat
     2341 ggcacaccgg tcactgccga agcagtaaaa ctctcttttg agcggctact aaaaatcggc
     2401 caggggccag cagaagcatt tcccaaagat ttaaagattg atgctcccga cgaacatacg
     2461 gtgaagttta cccttagcca gccattcgca ccgttcctct acacgctggc gaatgacggt
     2521 gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc
     2581 ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag
     2641 caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta
     2701 tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt
     2761 gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc
     2821 aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa cagcaaagcg
     2881 cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta tcagggcatg
     2941 gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg
     3001 tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa
     3061 tgggataaag tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg
     3121 aactgggagc ctattgcttt ggcgacacaa tccagtctca acaagctggg catcaatgtg
     3181 aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac
     3241 attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg
     3301 tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc
     3361 gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgggactac
     3421 cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac
     3481 taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag
     3541 gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg
     3601 ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca
     3661 tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt
     3721 ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta
     3781 cgtcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc gtccggtgct
     3841 ggaagagttg cgtatatttt tcccggcgac gctggagctg gctttttgtg ccctgctgct
     3901 ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa atcgctggct
     3961 ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt tctggcttgg
     4021 gctgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg gcggaaggct
     4081 tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgctgct
     4141 tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac
     4201 gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga tgctggaaca
     4261 attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt
     4321 tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg gtttggcgct
     4381 gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg
     4441 tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt
     4501 cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat
     4561 tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta
     4621 cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt
     4681 ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc tattgatgat gattttttca
     4741 ccgtggctgg cgacgcatga tcccaacgcc attgatttaa ccgcccgcct tttgccgcct
     4801 tctgcggcgc actggtttgg caccgatgaa gtgggacgcg atctgttcag ccgcgtactg
     4861 gtcggcagtc agcaatcaat tctcgccgga ttagtggtgg tcgccattgc gggtatgatt
     4921 ggttcgctac tcggatgtct atccggtgtg cttggcggac gcgcagacgc catcatcatg
     4981 cgcatcatgg acattatgct gtcgattcct tcgttggtac tgacaatggc actggctgcc
     5041 gctctcgggc cgagtttgtt taacgccatg ctggcgattg ctattgtgcg aattcccttt
     5101 tatgtgcgcc tggcgcgggg gcaagcatta gttgtacgcc agtataccta tgttcaggcg
     5161 gcgaaaacct ttggcgcgtc tcgttggcat ctgatcaact ggcatatttt acgtaactcc
     5221 ctaccgccgc tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt aatggccgcc
     5281 acgttgggat ttattggcct gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta
     5341 gcaaatggtc gcaactatgt actcgatcag tggtggtatt gcgcatttcc gggggcagcg
     5401 attttgctta ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga tctgcttgac
     5461 ccgaaagcag gaggaaagca gtcatgaccc aacccgttct ggacattcaa cagctgcatt
     5521 tgagtttccc cggttttaac ggtgatgttc acgcgctcaa caatgtgtcc ttgcagatta
     5581 accgcggcga aattgtcggt ctggtgggag aatccggctc aggtaaatca gtcaccgcaa
     5641 tgctgattat gcgtctgcta cagacgggca gttattgcgt acatcggggg cagatttcac
     5701 tgctgggaga cgatgttctt aacgctcggg aaaaacagct tcgtcagtgg cgcggcgcac
     5761 gagtggcgat gatctttcag gaaccgatga ccgccctcaa tccgacactt cgaataggtc
     5821 ttcagatgat ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa gccagagcta
     5881 aagcgattgc cctgctggaa gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct
     5941 atccgtttga gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg gcattctcct
     6001 gcgagccgca attgattatt gccgacgaac cgactacggc gctggacgtc acggtgcagt
     6061 tgcaggtact gcgtttgctt aaacataaag cccgcgccag tggaactgcg gttctgttca
     6121 tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg
     6181 gaagcgtaat tgaaagcggc gtgacggcag atgttatcca tcatccccgg catccgtata
     6241 ccattggttt gctccaatgc gcaccagaac atggagtacc acgccagcta ttacccgcca
     6301 ttccagggac ggtaccaaac ctcacccatt tgcctgaagg ctgcgctttt cgcgatcgtt
     6361 gctatgcggc aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca
     6421 accagcgctg cgcctgttgg tatccgcagc aggaggtcat aagtgtctga cacgttatta
     6481 acgttacgcg acgtccatat caatttcccg gcccgtaaaa actggcttgg taaaactacg
     6541 gaacatgttc atgccattaa tggtattgat ttacagatcc gccgtggtga aaccttaggg
     6601 atcgtcggcg agtcaggctg cggcaaaagc accctcgcac agcttttaat gggcatgctg
     6661 caaccgagcc acgggcagta catccgttca ggctcacaac gcattatgca gatggtgttt
     6721 caggacccgc tctcttcgct taatccgcgc ttaccggtgt ggcgcatcat cacagaaccg
     6781 ctctggatag ctaagcgtag tagtgaacaa cagcggcgag cgttagcaga ggagttggct
     6841 gtgcaggtgg gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg
     6901 cagcggcaac gcatcgccat tgccagagca ctctcttcgc agcctgacgt gattgtgctt
     6961 gatgagccaa cctctgcact ggatatctcc gtgcaggcgc agatcctcaa tttactggta
     7021 acgctacagg taaatcgcgg gctgacctat gtgctgatct cacacaatgt ctcggtgatt
     7081 cgtcatatga gcgatcgggt agcggtgatg tatctcgggc agattgtaga actgggcgac
     7141 gcgcagcagg tgttgacggc acctgcacat ccatacaccc gattattgct ggattccctc
     7201 cccgccattg ataaaccgct ggaggaagaa tgggcattac gtaaaacgga tctgccggga
     7261 aaccgcacgt tgccgcaagg ctgttttttc tacgaacgtt gccctctggc aacccacgga
     7321 tgtgaagtcc ggtaatcatt aacaataagg gaggacggac gtgagatccg gtgctggcgg
     7381 gcgctgtaga gatgctaaca acgcatcagg cattcacgcg cctgacgcgt catccggcaa
     7441 tgctttacga tttcaactgg taatcaagcg taatttccgc tttcagtacc tgagaaaccg
     7501 ggcatcctgc ttttgctttc tggatgatgc cgtcaaaggt agaggcatca atacccggca
     7561 ccgcaacttc actcttcagt gcgattttcg taatcgcaaa accggcatcc actttatcca
     7621 gcgacacatc ggcggtggta tcaatcgatg ttggcgtaaa tcccgcttcc cccagcatta
     7681 atgaaagcgc cattgagaaa catgcggcat gcgctgcgcc aatcagttct tcagggttag
     7741 ttcctttttc gccttcaaaa cgcgtgttaa atccatacgg ctgttggttc agcacaccac
     7801 tctcggtgga tactgttccc ttcccgcgtt tgatatcgcc ttcccagtgt gcctgacctt
     7861 tcttatggat tgtcattgtt gctctcctgt gggcttaaaa aggaaattat agactacgtg
     7921 agaaacagca cgaggataag caggatattc cgaataaaaa tccggcagaa atattacttc
     7981 tgcacttaaa ttaaatttcc acaatagata accgggaata tataacccaa gagatattgc
     8041 attaatatta atcaactcac cacaataata atacctatca atatcatgag gttaaaatat
     8101 aaaaatcatc aaccaggact aatcttaaca acgaagcggc aaatatttgc catgctgaat
     8161 gtgctttata aatctgcgat ccgtagcaga caccataaat acacagacac ggagaatcac
     8221 tatgtttact tattatcagg cagaaaattc aacagcagaa cccgctctgg ttaatgccat
     8281 tgagcaaggc cttcgggcag agcacggtgt tgtcactgaa gatgacattc tcatggagct
     8341 gaccaaatgg gtggaagctt ctgataacga catcctcagt gatatctacc agcaaacgat
     8401 caattatgtg gtcagtggcc agcaccctac gctttaaggt gctatgcttg atcggcaacc
     8461 taatttaggg gtttagcacg tgtttcttcg ctacggcgat gttgtcctta aaactagcaa
     8521 caggattgag gagttaaaat gaaatcgaac cgtcaggcac gtcatattct tggactggac
     8581 cataaaattt ctaaccagcg caaaatagtt accgaaggtg acaaatccag cgtggtaaat
     8641 aacccaaccg gcagaaaacg ccccgctgaa aagtaattca taaccatcag tcctcaatga
     8701 cgattaaaca ccattgcctg cgcaatggtg tttttgtttt tatctgcttt atacttgggg
     8761 ccgacgccct ggtggtaaag caaagacgat aaaagcgtgc cagggatgga tattcaaaaa
     8821 agagtgagtg acatggaacc aaaaacaaaa aaacagcgtt cgctttatat cccttacgct
     8881 ggccctgtac tgctggaatt tccgttgttg aataaaggca gtgccttcag catggaagaa
     8941 cgccgtaact tcaacctgct ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa
     9001 gcggaacgag catggatcca gtatcaggga ttcaaaaccg aaatcgacaa acacatctac
     9061 ctgcgtaaca tccaggacac taacgaaacc ctcttctacc gtctggtaaa caatcatctt
     9121 gatgagatga tgcctgttat ttatacccca accgtcggcg cagcctgtga gcgtttttct
     9181 gagatctacc gccgttcacg cggcgtgttt atctcttacc agaaccggca caatatggac
     9241 gatattctgc aaaacgtgcc gaaccataat attaaagtga ttgtggtgac tgacggtgaa
     9301 cgtattctgg ggcttggtga ccagggcatc ggtgggatgg gcattccaat cggtaaactg
     9361 tcgctctata ccgcctgtgg cggcatcagc ccggcgtata cccttccggt ggtgctggat
     9421 gtcgggacca acaaccagca gctgcttaac gatccgctgt atatgggctg gcgtaatcca
     9481 cgtatcactg acgatgaata ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa
     9541 cgctggccgg acgtgctgtt gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta
     9601 cttaaccgct atcgcaatga aatttgttct tttaacgatg acattcaggg cactgcggcg
     9661 gtaacagtcg gcacactgat cgcagccagc cgcgcggcag gtggtcagtt aagcgagaaa
     9721 aaaatcgtct tccttggcgc aggttcagcg ggatgcggca ttgccgaaat gatcatctcc
     9781 cagacccagc gcgaaggatt aagcgaggaa gcggcgcggc agaaagtctt tatggtcgat
     9841 cgctttggct tgctgaccga caagatgccg aacctgctgc ctttccagac caaactggtg
     9901 cagaagcgcg aaaacctcag tgactgggat accgacagcg atgtgttgtc actgctggat
     9961 gtggtgcgca atgtaaaacc agatattctg attggcgtct caggacagac cgggctgttt
    10021 acggaagaga tcattcgtga gatgcataaa cactgtccgc gtccgatcgt gatgccgctg
    10081 tctaacccga cgtcacgcgt ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt
    10141 aacgcgctgg tcgccactgg cagcccgttt aatccagtgg tatggaaaga taaaatctac
    10201 cctatcgccc agtgtaacaa cgcctttatt ttcccgggca tcggcctggg ggttattgct
    10261 tccggcgcgt cacgtatcac cgatgagatg ctgatgtcgg caagtgaaac gcttgctcag
    10321 tattcgccgc tggtcctgaa cggcgaaggt ctggtactac cggaactgaa agatattcag
    10381 aaagtctccc gcgcaattgc gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg
    10441 gtgaaaacgt ctgccgaagc tctgcaacag gccattgatg ataatttctg gcaagccgaa
    10501 taccgcgact accgccgtac ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc
    10561 tatttctctc tccctttttc agatctcatc catactgggt agtggcgaat aaatctcatt
    10621 tgcctcacct gctatgcaga acatcatccg aaaaggagga actatgaagg ctgcagttgt
    10681 tacgaaggat catcacgttg acgttacgga taaaacactg cgctcactga aacatggcga
    10741 agccctgctg aaaatggagt gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg
    10801 cgattttggt gacaaaactg gcgtaattct gggccatgaa ggcatcggtg tggtggcaga
    10861 agttggccct ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta
    10921 cgaaggatgc ggtcattgcg aatactgtaa tagtggtaac gaaacgctct gccgttcagt
    10981 taaaaatgcc ggatacagcg ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga
    11041 ttacgcggta aaagtgccag atggtctgga ctcggcggcg gccagcagca ttacctgtgc
    11101 gggggtcacc acctacaaag ccgttaaact gtcaaaaatt cgtccagggc agtggattgc
    11161 tatctacggt cttggcggtc tgggtaacct cgctctgcaa tacgcgaaga atgtctttaa
    11221 cgcgaaagtg atcgccattg atgtcaatga tgagcagtta aaactggcaa ccgaaatggg
    11281 tgcagattta gcgattaact cacgcaccga agacgccgcc aaaattgtgc aggagaaaac
    11341 cggtggcgct cacgctgcgg tggtaacagc agttgctaaa gctgcgttta actcggcagt
    11401 tgatgctgtc cgtgcaggcg gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag
    11461 cctggatatc ccacgccttg tgctggatgg cattgaagtg gtcggttcgc tggtcggcac
    11521 gcgccaggat ctaaccgaag ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt
    11581 cgccctgcgt ccgttagcgg acatcaacac catctttacc gagatggaag aaggcaaaat
    11641 ccgtggccgt atggtgattg atttccgccg ttaagaggcc tttgctgcga gtgccagtcc
    11701 tggtgtcgca gcatcgcact ctctccgaca ttaaatggct aaatggatta ttgttccttc
    11761 ctacgcaaca ataatgatag tgttcctgat gaaggaacac tactgagagt aaagacactg
    11821 atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact ggacgaactt
    11881 aatgtcagcc tgcgcgagtt tgccagagca atggaaattg ctccctcaac ggcaagccga
    11941 ttgctgaccg gaaaagcagc tttgacacca gaaatggcga ttaaactttc cgtggtgatc
    12001 ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagtctggc agaggcagaa
    12061 aaaacggtgg atgtgtcgcg actacgccgt ctggttacgc aataaaaaaa tggcgcttct
    12121 taaagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc actctctttt
    12181 ttcgcttctg ccttctcgat ttcacgatac cagcgcggat ggtgtttctt cgcccagcga
    12241 cggctcacct tcccttcgat catcccttta atcgaccctt tcacccagaa tgccatatac
    12301 atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcgtggat cagcaggctg
    12361 tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg gacgccagat aatcaccccg
    12421 gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt ttgcccggcg
    12481 ttgtacttac cgacatccgc cactttatgc tcattgcctt tcaatacttc gacaatgttc
    12541 aacagccacg gaatatcttt cttatccggg atgttgtgat gcacaaaacg cacaaacata
    12601 aacatcagtg cgacgaaaat cgcaatgccg aaaaacgggt gcaaaatgcg tcccatctgc
    12661 ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga aatcccggac
    12721 agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc aataaatttg
    12781 gtgcgcacaa tcattttcga cttactcatg atgatcctcc tcgtcatcgt ccacttcctt
    12841 attcgggcca ataccgatgt agtggaaaat caacccggca aaagtggcaa taaagccagc
    12901 cgctgccagc ggtttcaacg cgcctttcca caggcttacc gaggtgtcga tcttcggatc
    12961 tttcggcaga ccgtgataca gctccggctg atcggcgtga tgcagcacgt acataacgtg
    13021 cgtaccaccg accccttccg ggttgtagac gccagcatgt tcgtaaccac gcgctttcag
    13081 tttcgccacg cgctgttccg ccagctccag catctccttc ttggtgccga agtggatagc
    13141 cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc gatcgacgca
    13201 gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat taaacggaca
    13261 cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcga caatcccgtt
    13321 agcgtactga atgattgcac cagcagacgg gcacgccttc aggcagccgg gatcttcaca
    13381 gtgcatacag ccgtctttac ggatcagcca ctccagcttg ccgttctgtt cggtttcgct
    13441 aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt aaaccccgac
    13501 gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa cacgccacct gacaggcttt
    13561 acagccgata caggtggaaa cgtcgataag ttttgcgact tctgctttgt aatcacgcac
    13621 ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatgtcct gcgtttccat
    13681 agccatctgt tcgccccctt acgccttctc gatgttgact aagaacgctt tatattccgg
    13741 cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagcgatat aacctttacg
    13801 cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct gctgaccatt
    13861 tacattcagt ggtttcagac gacgcgttac cac
//
LOCUS       DAFATA010000112        13867 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFATA010000112 DAFATA010000000
VERSION     DAFATA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.113"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..144)
                     /locus_tag="LGJ20_003970"
     CDS             complement(<1..144)
                     /locus_tag="LGJ20_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBK2387976.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKS"
     gene            complement(163..564)
                     /locus_tag="LGJ20_003971"
     CDS             complement(163..564)
                     /locus_tag="LGJ20_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HBK2387977.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(691..1422)
                     /gene="rlmB"
                     /locus_tag="LGJ20_003972"
     CDS             complement(691..1422)
                     /gene="rlmB"
                     /locus_tag="LGJ20_003972"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK2387978.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1602..4043)
                     /gene="rnr"
                     /locus_tag="LGJ20_003973"
     CDS             complement(1602..4043)
                     /gene="rnr"
                     /locus_tag="LGJ20_003973"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK2387979.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(4082..4507)
                     /gene="nsrR"
                     /locus_tag="LGJ20_003974"
     CDS             complement(4082..4507)
                     /gene="nsrR"
                     /locus_tag="LGJ20_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBK2387980.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4712..6010)
                     /gene="purA"
                     /locus_tag="LGJ20_003975"
     CDS             complement(4712..6010)
                     /gene="purA"
                     /locus_tag="LGJ20_003975"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK2387981.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(6114..6311)
                     /locus_tag="LGJ20_003976"
     CDS             complement(6114..6311)
                     /locus_tag="LGJ20_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBK2387982.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6393..7397)
                     /gene="hflC"
                     /locus_tag="LGJ20_003977"
     CDS             complement(6393..7397)
                     /gene="hflC"
                     /locus_tag="LGJ20_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBK2387983.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7400..8659)
                     /gene="hflK"
                     /locus_tag="LGJ20_003978"
     CDS             complement(7400..8659)
                     /gene="hflK"
                     /locus_tag="LGJ20_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBK2387984.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8745..10025)
                     /gene="hflX"
                     /locus_tag="LGJ20_003979"
     CDS             complement(8745..10025)
                     /gene="hflX"
                     /locus_tag="LGJ20_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK2387985.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(10101..10409)
                     /gene="hfq"
                     /locus_tag="LGJ20_003980"
     CDS             complement(10101..10409)
                     /gene="hfq"
                     /locus_tag="LGJ20_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBK2387986.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10495..11445)
                     /gene="miaA"
                     /locus_tag="LGJ20_003981"
     CDS             complement(10495..11445)
                     /gene="miaA"
                     /locus_tag="LGJ20_003981"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK2387987.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11438..13285)
                     /gene="mutL"
                     /locus_tag="LGJ20_003982"
     CDS             complement(11438..13285)
                     /gene="mutL"
                     /locus_tag="LGJ20_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK2387988.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(13295..>13867)
                     /locus_tag="LGJ20_003983"
     CDS             complement(13295..>13867)
                     /locus_tag="LGJ20_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK2387989.1"
                     /translation="VARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWL
                     EQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGE
                     IHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNY
                     FLAHPMQSAPQGATAQTASTVTTPDRTLPN"
BASE COUNT         3136 a         3930 c         3417 g         3384 t
ORIGIN      
        1 agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg
       61 cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt tccccagcgt
      121 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc
      181 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
      241 ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt cctgttgcac
      301 gctggagatt cctaccgatg acaacggcat catcttctga tttcttaata agaaggtatt
      361 aaattcatct ggattgctga tactacttac cggacaaata aaggtttcga taatcatttg
      421 gcgggaagta aaaagaatat tgatttgcaa atcgccataa tcattcattt taataattaa
      481 tgcgctctcg ctatttgtca catcaatatt ttgttcaggt acagtttgca gcgccgtcgc
      541 cagcgccaac ggattccatg tcatatatta ttccatatag atttagttta aatattaata
      601 aaatgaatat ttgcagtacg taattatctt accagctata gacaaaaaaa aaccatccaa
      661 atctggatgg cttttcataa ttcagacgaa ctagctgcgc tgacgaaccg cttcaaataa
      721 gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca ttgggatgct
      781 gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat
      841 caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc
      901 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg caaggttagt
      961 cacccgaatc agtggaacgc tttctgccgc gccgcaggct acttttttcg ccgtagcgtt
     1021 gagctgtgcg gagcgatctt tcggcacaat caccgcgtga acgccagcag cgtccgcgct
     1081 acgcaggcac gcgccgaggt tgtgcggatc ggttacaccg tcgaggatca gcaggaacgg
     1141 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac gtcctggctt
     1201 cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatattggcg
     1261 gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg
     1321 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
     1381 cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
     1441 cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata tattgaattt
     1501 gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcatt
     1561 ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc cacttttttc
     1621 ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt
     1681 ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc accgcggaag
     1741 gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc gccttttttg
     1801 cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt
     1861 tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga
     1921 acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat caggcgttgc
     1981 cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca
     2041 atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga aattacgcct
     2101 ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc agcgacatcg
     2161 cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcaacatc
     2221 tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg cccctgctct
     2281 ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc
     2341 gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttctggatcg
     2401 taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc atcaggacgg
     2461 tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt accacccggc
     2521 agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc
     2581 ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag
     2641 atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg ctgggtctgt
     2701 tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc gctctcaaat
     2761 gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct
     2821 tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata
     2881 tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc gtagaatttg
     2941 tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct
     3001 acctgcgggt tgagcgagca caggccgtta gagagcactt ccggcagcat cgggataacc
     3061 tgcgaaggga agtacaccga cgtaccacga ttacgcgctt ctctgtccag cggcgttggc
     3121 ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc gccgccgcgt
     3181 tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc
     3241 agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac ttcttctttc
     3301 agcccggcaa cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc
     3361 agagcgatat caaccgccat gccggtgccc atattatcgc caaggacttc gacgatttta
     3421 cccaccgctt tggtacggcg agtcgggcgt tgggtcagtt caacaaccac cacaaagccc
     3481 atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag acggctgtcg
     3541 tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt
     3601 tttggcacca gtacacggac aatacgcgct tcacgacgac ctttacggtc agcgcccagc
     3661 ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac
     3721 aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg gccaataacg
     3781 gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg agtgaagacc
     3841 agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg
     3901 ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt
     3961 aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt
     4021 tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc
     4081 gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg
     4141 tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc
     4201 gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca
     4261 gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg cgaatgccgc
     4321 cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga
     4381 ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc
     4441 cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta
     4501 actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg atgataaacc
     4561 agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa
     4621 cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg
     4681 gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacggatcg cgcagaatca
     4741 tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacacca gtcagctctt
     4801 caatacgctt gatgtagttc agcgccgcct gcggcagacc gctacgatct ttcacgccga
     4861 aggtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt
     4921 cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa gccacgcaga
     4981 gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacaggg
     5041 agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac
     5101 gccccgtagt tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa
     5161 acagttcggt cgggaacgga cctgcaccta cacgagtgga gtaagctttg aggataccca
     5221 gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg
     5281 tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc gtaccctgcg
     5341 caccttcaaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt
     5401 caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt
     5461 aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt
     5521 ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac
     5581 gtgctacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg atcgctttcg
     5641 cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac
     5701 atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca
     5761 tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg ctggttacat
     5821 tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga
     5881 gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca
     5941 gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt
     6001 tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca
     6061 gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca
     6121 atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa
     6181 cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc
     6241 gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt
     6301 gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg
     6361 tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc
     6421 atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag
     6481 ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc tttactgaat
     6541 gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcatgat gcggccctga
     6601 cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc
     6661 gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata
     6721 cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat acgcacatcg
     6781 acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc
     6841 gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatccgctgc cggggtagta
     6901 acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga
     6961 cgaccacggg aatcggtgac gatgtctttc acgtccaggc gaccaatttc agaacgcaga
     7021 cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt
     7081 gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg
     7141 tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg
     7201 tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagacccgg ctcataaacc
     7261 agaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca
     7321 ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg
     7381 ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg
     7441 cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg
     7501 ttgtggagga agaggctggc ggcaaacgca gcagattgct ggcaccgtta tcgctcttcg
     7561 ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc aggttgccac
     7621 ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc gtctcgatat
     7681 acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc
     7741 gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga
     7801 ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt
     7861 cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta
     7921 cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata cccatgtcat
     7981 acggacggat agtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt
     8041 ccgtcagaat gcggtccatg gtgtatttac cgataacccc gcgcagggcg ctgtcggtag
     8101 cctgacgcag actgtcatcc gggctggtca cgctatacag atatttttcc ggattggtaa
     8161 cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc aacatcacac
     8221 cggaagcggc cagttcacgc acggcttcca cgtttaccgg tttgacttcg tcgataaacg
     8281 tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca
     8341 cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg
     8401 ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac
     8461 tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt
     8521 tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc
     8581 cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac
     8641 cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct
     8701 ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg
     8761 atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc
     8821 agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa
     8881 cgacttctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg
     8941 gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt ctgtgcggaa
     9001 agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc
     9061 agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca
     9121 agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg
     9181 tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc tgcaaccaga
     9241 tcgtgcggca ggtggcgaat aaaccctacg gtatctgcca acacggtttc accgacatct
     9301 gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag
     9361 acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc
     9421 agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt
     9481 tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg
     9541 gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg
     9601 tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc
     9661 aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg
     9721 tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca
     9781 tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct
     9841 ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc
     9901 aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct
     9961 ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca
    10021 aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg
    10081 taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc ggaagtattc
    10141 tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt gttactgtga
    10201 tgagaaaccg gacgagacgg gacaacagta gaaatcgcgt gcttgtaaac catctggctg
    10261 accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc ttgcagctta
    10321 ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc gttcaggaac
    10381 ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct tatttgtact
    10441 ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac acattcagcc
    10501 tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt tttcactgtc
    10561 aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg ccaactgtct
    10621 cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt caaggtaaga
    10681 ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca aatctcctcg
    10741 ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat gaaaacgctg
    10801 ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact gatgcacctg
    10861 atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac ccgaaatgaa
    10921 aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc ttgctgccgc
    10981 aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct ctgccgcctg
    11041 ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca acccttccag
    11101 caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc ccgccgcggt
    11161 gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt aagcctgcga
    11221 cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag cgttcggctt
    11281 cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta tcaactctac
    11341 tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg tcggccccat
    11401 caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc agggctttta
    11461 tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc acaagttgcg
    11521 gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac cactgcgcat
    11581 gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg aatacggact
    11641 gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt tggcgtaagg
    11701 gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca atacccaatt
    11761 ccgccagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa acttttagcc
    11821 gcaacggaat cagcaacggc tgggcgcaaa ctggcgcttc acccggcgtc agttgcgcct
    11881 gacgcagcca acgttctgcc actggcaagg ctaaaagtga aatgttgccg tcgcgctcca
    11941 gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga ctgttcgccg
    12001 caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg ggcgtttgca
    12061 aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca ttcggccagg
    12121 gggcagccgg acgactgcct gatgctggcg caggagtgta acgcggagct accggctccc
    12181 gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc gggatggcac
    12241 gcggtgcagg ttggggttca tcgtccagcg gtagcggcgt ttccagttgc tgctgtaaca
    12301 cgctcagcac gccttgatag ataaagtcat gcaccagacg cgactgatga aaacgcactt
    12361 cgtgtttggc ggggtgcacg ttgacgtcca cctgatgtgg gtcgatctcc agatacagca
    12421 caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg atcgcgtgat
    12481 tgatcaggcg atcgcgcatc atgcgaccgt tcacgtagca atactgaatt tctgccagtg
    12541 cgggcgtggt gtgatttgga tcggccaccc agccgcgtag cgtgagatcg ccgtgttgcc
    12601 attcaatcgc cagcgcttgt tcaagaaaag cggtgccgca aatcgcgcct aagcgccgtt
    12661 ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg ttatgcgaca
    12721 ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatctca tcaatgtggt
    12781 taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg tagaacagat
    12841 ccagcacctc cagcgtcgtc cccacaggat gcgccgccgg ttttaccgtc acgttcatat
    12901 cgcgcccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt gaagtgagcg
    12961 tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc aggctgataa
    13021 tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgcgcc agcgccagcg
    13081 ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt ttcgccccac
    13141 cgcgttcgat atcaatatcg atacgcgtcg cgcctgcatc gaggctgttt tccaccagtt
    13201 ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg ttcgccagtt
    13261 gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg cgatctggcg
    13321 tcgtcaccgt actggcagtt tgtgccgttg ccccctgcgg cgcagattgc atcggatgcg
    13381 caaggaaata attgcgcagg cctttgtaaa tggcttctgc cagctgttgt tggtaatcgt
    13441 cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg accagtactg
    13501 acgggatatc cggcgaacgc agaacgccga gactcgcgtg ttctggtcgg cgtttatgta
    13561 tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac cctacccgtt
    13621 gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca gactgactgt
    13681 tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc tgctccagcc
    13741 agctggccat ttcgctgttg gcgcgacggt tagagagcac ccatacggaa gcgccagtcg
    13801 cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg ttttgcttac
    13861 gtgccac
//
LOCUS       DAFATA010000113        13776 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFATA010000113 DAFATA010000000
VERSION     DAFATA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.114"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1044
                     /gene="aroC"
                     /locus_tag="LGJ20_003984"
     CDS             <1..1044
                     /gene="aroC"
                     /locus_tag="LGJ20_003984"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK2387990.1"
                     /translation="FGESHGLALGCIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQ
                     RREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQKYG
                     LRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQMGDIPLEIKDWSLVE
                     QNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAH
                     ALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGILGGISSGQQIIAHM
                     ALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQR
                     AQNADVKTDIPRW"
     gene            1048..1872
                     /gene="mepA"
                     /locus_tag="LGJ20_003985"
     CDS             1048..1872
                     /gene="mepA"
                     /locus_tag="LGJ20_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBK2387991.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            1872..2681
                     /locus_tag="LGJ20_003986"
     CDS             1872..2681
                     /locus_tag="LGJ20_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK2387992.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            2681..3229
                     /gene="epmC"
                     /locus_tag="LGJ20_003987"
     CDS             2681..3229
                     /gene="epmC"
                     /locus_tag="LGJ20_003987"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBK2387993.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            3263..3541
                     /locus_tag="LGJ20_003988"
     CDS             3263..3541
                     /locus_tag="LGJ20_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBK2387994.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(3662..5668)
                     /gene="mnmC"
                     /locus_tag="LGJ20_003989"
     CDS             complement(3662..5668)
                     /gene="mnmC"
                     /locus_tag="LGJ20_003989"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBK2387995.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            5827..7047
                     /gene="fabB"
                     /locus_tag="LGJ20_003990"
     CDS             5827..7047
                     /gene="fabB"
                     /locus_tag="LGJ20_003990"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBK2387996.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            7358..8536
                     /locus_tag="LGJ20_003991"
     CDS             7358..8536
                     /locus_tag="LGJ20_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2387997.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIVVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(8533..9528)
                     /gene="flk"
                     /locus_tag="LGJ20_003992"
     CDS             complement(8533..9528)
                     /gene="flk"
                     /locus_tag="LGJ20_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBK2387998.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(9850..10146)
                     /locus_tag="LGJ20_003993"
     CDS             complement(9850..10146)
                     /locus_tag="LGJ20_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain and TM2 domain-containing
                     protein"
                     /protein_id="HBK2387999.1"
                     /translation="MSEMVFCRGCGKEIHKDATSCPNCGAPQRTASSKSRMTAALLAF
                     FLGGLGAHKFYLGKVGQGFLYLIFCWTFIPAIVAFIEFFIYLCTSDEDFARKYG"
     gene            complement(10282..10422)
                     /locus_tag="LGJ20_003994"
     CDS             complement(10282..10422)
                     /locus_tag="LGJ20_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBK2388000.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(10613..10873)
                     /locus_tag="LGJ20_003995"
     CDS             complement(10613..10873)
                     /locus_tag="LGJ20_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HBK2388001.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKQASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            11065..12087
                     /locus_tag="LGJ20_003996"
     CDS             11065..12087
                     /locus_tag="LGJ20_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388002.1"
                     /translation="MGRDITLYPKKATRNELKNYLENLGFKRCKHLWEWPQGTLNYSW
                     FDDTDFKSTDGVSADIYPVSNDELHISGNKWALHVRNLSSASWHDVKMLNDVLKGARS
                     LFGGTLIGDYGKNRYAPLWEDSSSPISRGISSIFNHVHHEISAVKHALPEPSIKLNLP
                     EDGGLSEYFDDMQCMDPSRVIYNGLVPFAVAMFEYFFSRAFQILIKYDPFAIAKRTSY
                     KQKVDFDILLEIEKGNISIESVIARNYTFQNLTLLNKAYKEWLDIDVRAILYKKKRIG
                     KSVDYLENRISEIIQYRHGIVHHFELDRTLNRDGYIHILDAIEKSIIEFIHYIESKYK
                     FKLNAY"
     gene            complement(12208..13317)
                     /locus_tag="LGJ20_003997"
     CDS             complement(12208..13317)
                     /locus_tag="LGJ20_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBK2388003.1"
                     /translation="MTDTTMQLLSQGTAPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSDIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            13475..>13776
                     /locus_tag="LGJ20_003998"
     CDS             13475..>13776
                     /locus_tag="LGJ20_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBK2388004.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAISDQVSTKVIVVRVAEHKEEDGKTQDQL"
BASE COUNT         3189 a         3494 c         3624 g         3469 t
ORIGIN      
        1 ttcggcgaat cgcacgggct ggcgctcggc tgcatcgtcg atggtgttcc gccaggcatt
       61 ccgctgacgg aagcggacct gcaacatgac ctcgaccgtc gtcgccctgg gacatcgcgc
      121 tataccaccc agcgccgcga gccggatcag gtcaaaattc tctccggtgt ttttgaaggc
      181 gttactaccg gcaccagcat tggcttgttg atcgaaaata ccgaccagcg ttctcaggat
      241 tatagtgcga ttaaggacgt tttccgtcca ggccatgccg attacaccta cgaacaaaaa
      301 tacggtctgc gcgattatcg cggcggtgga cgttcttccg cccgcgaaac cgccatgcgc
      361 gtggcggcag gggcgattgc caaaaaatat ctcgccgaga aatttggtat tgaaatccgc
      421 ggctgcctga cccagatggg agacattccg ctggaaatca aagactggtc gctggttgaa
      481 caaaatccgt tcttctgccc ggacccggac aaaatcgacg cgttagatga actgatgcgc
      541 gcgctgaaaa aagagggcga ctccatcggc gcgaaagtca ccgttgttgc cagtggtgtc
      601 cccgccggac ttggcgagcc ggtctttgac cgcctggatg ccgacatcgc ccatgcgctg
      661 atgagcatca acgcggtgaa aggcgtggaa attggcgacg gttttgacgt agtggcgctg
      721 cgcggcagcc agaaccgcga cgaaatcacc aaagacggtt tccagagcaa ccatgcgggc
      781 ggcattctcg gcggtatcag cagcgggcag caaatcattg cccatatggc gctgaaaccg
      841 acctccagca ttaccgtgcc ggggcgcact attaaccgct ttggcgaaga agttgagatg
      901 atcaccaaag gccgtcacga tccctgtgtc gggatccgcg cggtgccgat cgctgaagcg
      961 atgctggcga tcgttttaat ggatcacctg ctacggcagc gggcgcaaaa tgccgatgtg
     1021 aagactgata ttccacgctg gtaaaaaatg aacaaaaccg cgattgcgct gctggctctg
     1081 cttgccagta gcgctagcct ggcagcgacg ccgtggcaaa aaataaccca acctgtgccg
     1141 ggtagcgcac aatcgatagg cagtttttct aatggctgta tcgtcggcgc tgacacgctg
     1201 ccgatacagt ccgaacatta tcaggtcatg cgtaccgatc agcgtcgcta tttcggtcac
     1261 ccggacctgg tgatgtttat ccagcgtctg agtagccagg tgagcaatct gggcatgggt
     1321 acggtgctga ttggcgatat ggggatgcct gctggtgggc gtttcaacgg cggtcatgcc
     1381 agccaccaga ccggactgga tgtcgatatc tttctgcaac tgccgaaaac tcgctggacc
     1441 tccgcgcagc tcttgcgccc gcaagcactg gacttagttt cccgcgacgg taaacacgtt
     1501 gtctccacgc tgtggaagcc agaaattttc agcttgatca aactcgccgc ccaggacaaa
     1561 gacgtcacgc gcatttttgt taatccggcg attaaacaac aactttgcct tgatgcgggc
     1621 accgatcgcg actggttgcg caaagtgcga ccctggttcc agcatcgcgc gcatatgcat
     1681 gtacgattac gttgtcctgc cgatagtctg gagtgtgaag atcaaccttt accgccacca
     1741 ggcgatggtt gcggggcaga actgcaaagc tggtttgaac ctccaaaacc gggaacaaca
     1801 aagcctgaga agaagacacc gcctccgttg ccgccttcct gccaggcgct actggatgag
     1861 cacgtgatct aatggaaacg tttaatagcc tgtttatggt ttccccgctg ttgctgggag
     1921 ttctcttttt tgtcgccatg ctggcgggat ttatcgactc gattgccggt ggcggtgggt
     1981 tactcaccat tccggcattg atggctgcgg gaatgtctcc cgctaacgcg ctggcaacca
     2041 ataaactgca agcctgcggc ggctctattt ccgccactat ctactttatt cgccgcaaag
     2101 tggttagttt aagcgatcag aaactcaata tcgccatgac ctttgtcggc tcaatgagcg
     2161 gcgcactgct ggtgcaatac gttcaggctg atgtcttacg gcagattttg cccattctgg
     2221 taatttgtat cggcctctat ttcttgctga tgccaaagct gggtgaagaa gatcgccagc
     2281 gtcggatgta cggtttaccc ttcgcgttaa ttgccggtgg ctgcgtcggt ttttatgacg
     2341 gattctttgg tccggcagcc ggatcgtttt acgcactggc ctttgttacg ctgtgcggat
     2401 tcaacctcgc caaagccacg gcgcacgcca aattactcaa cgcaacgtca aacatcggcg
     2461 gtttactgct gtttattctc ggtggcaaag tgatttgggc gacgggcttt gtcatgctgg
     2521 tggggcagtt cctcggcgcg cgcatgggtt cacgactggt gttgagcaaa ggacaaaagc
     2581 tgatccgccc gatgattgtt attgtctcag cggtgatgag tgccaaatta ctttttgata
     2641 gccacggaca ggagatcctc cactggttgg ggatgaacta atgaacagta cacaccacta
     2701 tgagcagttg attgaaattt ttaatagctg ctttgccgat gaatttaata cccgtctgat
     2761 taaaggcgac gacgaaccga tctatcttcc tgctgatgcg gaagtaccgt ataaccgaat
     2821 cgtctttgct catggctttt atgccagcgc aattcatgag atttcgcact ggtgtattgc
     2881 cgggaaagcg cgccgtgagc tggttgattt cggctactgg tattgcccgg atgggcgtga
     2941 tgcccaaacg caaagccagt ttgaagatgt tgaagtgaag ccgcaggcac tcgactggtt
     3001 gttctgtgtg gcggcgggat atccgtttaa cgtcagttgc gacaatctgg aaggcgactt
     3061 cgaaccggat cgtgtggtgt ttcaacggcg ggtacatgcg caggtgatgg attacctagc
     3121 aaacggcatt cctgaacgcc cggcacgctt cattaaagca ctacaaaatt attatcacac
     3181 gccggaactt acggcggaac agttcccgtg gccggaagcg ctcaactaaa gccagcgcca
     3241 cgttttacaa tgaggaaaga agatgatcgc ggagtttgaa tcacgcattc tggcattaat
     3301 cgacggtatg gttgaccatg ccagtgatga tgagttgttt gccagtgggt atttgcgtgg
     3361 ccacctgaca ttagccatcg cagaactgga aagtggtgat gaccactccg ctcaggcggt
     3421 gcatacgacc gttagccaga gtctggaaaa agccattggt gccggtgaat tgtcgccgcg
     3481 tgaccaggcg ctggtgaccg atatgtggga aaacctgttt cagcaggcgt cacagcagta
     3541 agttgcggcg tctggagcac catgcctgat gcgacgctta tcgcgtctta tcaggcctac
     3601 acgtcacacg taggtcggat aaggcgttca cgccgcatcc ggcaaacact gcgagagcag
     3661 attaccccgc cttaaccgct ttacccttca acaatttccg cacccataac cgattcgggt
     3721 ttaacgccgc cagcgtactg gcatccatcg gaatcggttc gtcgctcatc tgcgccgcca
     3781 gaatctcggc acacagcggg gcagaacaca aaccgcgaga acctaaagcc gcaaacataa
     3841 agagatcgtc aaaaaccggc gcgcttaccg cctcatcttt ctgctccgcc aacgacgcat
     3901 attccacgag tgttgcctca taatcgggaa cattgcctac cattggcaga tgatcgcggg
     3961 tggcacaacg cacaccgcag cgcgcctctt tatcactgac atcaacctct tttgcccact
     4021 gtgcctgcgg gaaacaatca atcaaccgct ggcgattctg ctgctgatcg tcctcactgt
     4081 acgccgtatc ttcgctgccg cgatgataac tggcaccaat acaatgatgt tgattcgccg
     4141 gattttgtgg cgtgagataa ccgtcatagc acagcacctg cttcagctct gccagttccg
     4201 gcgttgtcgg aatatggctg acctgcccgg caaccgaata caccgggaga gtcgacgttt
     4261 ggctgaatcg gctgatttga tgcccgttcg ccagtaccac tacgctgtgt gttgcttgct
     4321 gatctcctgc aaaattcaac aaccaacagt catccttacg ggataaattc tgtaactgat
     4381 attgataata aatctgcaaa ccctgctgtt gcgccagttc cagcacatta cgggtcagtt
     4441 ctgctgggca cagccaacca ccttgcggat aagtaatgcc gctgcaattt gtcgcaacgc
     4501 ccgtaatttg ttcaaccgca tttgcctcaa cggctacagc cagttctgcg ggtaaatcca
     4561 ttgacaacat ctgtgcgatt ttatgctggc ttttctcatc ccagcctaac tgcgtgacgc
     4621 cgcaccagtc atgatcaaat ttaacgggta attggtcgta aaaccgacga gcaaaagtaa
     4681 acgcattaga gaaaaagcgg tttagcgcct catcgtgttt gcttaataac ggatacagcg
     4741 ccccctggcg attgccggaa gcacccagtg cgggggcctc atccgcgcaa taaagcgtta
     4801 cctgccagcc gcgccgtaat agcgccagcg acaacaacgc gctggcaata ccaccgccga
     4861 taatcgccgc ttcccgtttg ctgctgcccg tgcggttaaa ccacggcgcg gagcagggga
     4921 gcggtaatgt ctgttccatc accccgcaaa gcatttcccg tttgcgccca aagcccttac
     4981 gtttttgcat cgtgaatccg gcgtcctgca aaccgcggcg gacaaaaccg gcagacgtaa
     5041 atgtcgccag cgtgccgccc ggacgcgcca accttgccat ggcgttaaac agattttgcg
     5101 tccacatatc cgggtttttc gctggcgcaa agccgtccag aaaccaggca tctacttttt
     5161 gatttagcga atcgtccagt tggctggtca gttcgttaat atcgccaaac cataaatcca
     5221 gcgtcacgcg gccttcatcg agcaataaac gatggcaacc gggcaagggc attggccact
     5281 gcgcctgaag ttgttctgcc cacggagcca gttccggcca gtgttgatgc gctaaggcta
     5341 aatccgcacg ggtgagggga aatttctcaa aactaatgaa atgtaagcgt tgtaattgcg
     5401 cttgcggatg cgcttcgcga aactgatcaa atgcctgcca tagcgtcagg aagtttaatc
     5461 cggtgccgaa gccgctctct gctaccacaa acagaggatg tggatgctca ggaaagcgta
     5521 cctctaattg gttgcctccc agaaaaacat aacgcgtctc ttccagcccg ttatcgttgg
     5581 aaaaatagac atcgtcaaaa tctcgggaaa caggtgtacc ctcagcatta aattcgaggt
     5641 tggcaggttg tatggagtag tgtttcacgt aagttactcg tcttacaggc ggtggctcga
     5701 tcttagcgat gtgtgtaagg ctgcgcaaat ttctctatta aatggctgat cggacttgtt
     5761 cggcgtacaa gtgtacgcta ttgtgcattc gaaacttact ctatgtgcga cttacagagg
     5821 tattgaatga aacgtgcagt gattactggc ctgggcattg tttccagcat cggtaataac
     5881 cagcaggaag tcctggcatc tctgcgtgaa ggacgttcag ggatcacttt ctctcaggag
     5941 ctgaaggatt ccggcatgcg tagccacgtc tggggcaacg taaaactgga taccactggc
     6001 ctcattgacc gcaaagttgt gcgctttatg agcgacgcat ccatttatgc attcctttct
     6061 atggagcagg caatcgcaga tgcgggcctt tctccggaag cttaccagaa taacccgcgc
     6121 gttggcctga ttgcaggttc tggcggcggt tccccgcgtt tccaggtgtt cggcgctgac
     6181 gcaatgcgcg gcccgcgcgg cctgaaagcg gttggcccgt atgtggtcac caaagcgatg
     6241 gcatccggcg tttctgcctg cctcgccacg ccgtttaaaa ttcatggcgt taactactcc
     6301 atcagctccg cgtgtgcgac ttccgcacac tgtatcggta acgcagtaga gcagatccaa
     6361 ctgggcaaac aggacatcgt gtttgctggc ggcggcgaag agctgtgctg ggaaatggcc
     6421 tgcgaattcg acgcgatggg tgcgctgtct actaaataca acgacacccc ggaaaaagcc
     6481 tcccgtactt acgacgctca ccgtgacggt ttcgttatcg ctggcggcgg cggtatggta
     6541 gtggttgaag agctggaaca cgcgctggcg cgtggtgctc acatctatgc tgaaatcgtt
     6601 ggctacggcg caacctctga tggtgcagac atggttgctc cgtctggcga aggcgcagta
     6661 cgctgcatga agatggcgat gcacggcgtt gataccccaa tcgattacct gaactctcat
     6721 ggtacttcga ctccggttgg cgacgtgaaa gaactggcag ctatccgtga agtgttcggc
     6781 gataagagcc cggcgatttc tgcaaccaaa gccatgaccg gtcactctct gggcgctgct
     6841 ggcgtacagg aagctatcta ctctctgctg atgctggaac acggctttat cgccccgagc
     6901 atcaacattg aagagctgga cgagcaggct gcaggtctga acatcgtgac cgaaacgacc
     6961 gatcgcgaac tgaccaccgt tatgtctaac agcttcggct tcggcggcac caacgccacg
     7021 ctggtaatgc gcaagctgaa agattaattc gcagtaggcc ggagtagtcg gagtagtcgg
     7081 agtagtcgga gtagtcggag taggtcggat aagacgcgcc agcgtcgcat cctacgttac
     7141 gcgctaatgc ggcctccggc actaacgcaa aagggaacct gatggttccc tttttcacat
     7201 cattgacaat cgccgcctgt tccaggcaaa cttcccgctt tgtcgatttc cttctgaaaa
     7261 gacgtacgcg ttaaatcctg ccaacgcact gtaaccctga aaccagagag atgagacggg
     7321 gatactcctc gccttgcgct gcattctgga gtaatgcatg actgctgtaa gccaaaccga
     7381 aacacgatct tctgccaatt tttcgctctt ccgcatcgct tttgcggttt ttctcaccta
     7441 catgaccgta gggctgccgt tgccggttat cccgctgttt gttcatcatg atctgggcta
     7501 tggcaatacc atggtcggga ttgtcgtcgg gattcagttt ctggctacgg tgctgacgcg
     7561 tggctatgcc gggcgactgg ccgatcaata tggcgcaaaa cgttcggcgc ttcaggggat
     7621 gttagcttgt ggtctggctg gcggcgcgtt gctgctggcg gcgattttgc ctgtctccgc
     7681 accgttcaaa tttgccctgt tggtcatcgg gcgtttgatt cttgggtttg gtgaaagcca
     7741 gttactgaca ggcgctctga cctgggggtt aggcatcgta ggggcaaaac actctggcaa
     7801 agtgatgtca tggaatggaa tggcgattta cggtgccctc gctgttggtg ctccgcttgg
     7861 cctgttgatt catagccatt acggttttgc cgcactggcg atcaccacaa tggtattacc
     7921 cttactggcg tgggcctgta acggcacagt gcgcaaagta ccggccctgg cgggagaacg
     7981 tccatcgctg tggagcgttg tcgggtttat ctggaaacca gggttagggc tggcactaca
     8041 aggcgttggt tttgcggtta tcgggacttt cgtttcgctc tactttgcca gcaaaggatg
     8101 ggcgatggcg ggctttactc ttaccgcgtt tggcggcgca tttgtcgtga tgcgcgtcat
     8161 gtttggctgg atgccggacc gttttggcgg cgtgaaagtg gcgattgtct ctctgcttgt
     8221 agaaacggtg ggcttgttgt tgctctggca agccccaggt gcgtgggtcg cattagcggg
     8281 cgcggcgtta accggagccg gatgttcgct tatctttcct gcgctgggcg tggaggtggt
     8341 taaacgcgtc ccctcacaag ttcgcggcac cgcactgggc ggttacgccg cgtttcagga
     8401 tatcgccctc ggcgtctccg ggccgctggc gggaatgctg gcgaccacgt ttggttactc
     8461 ttcggtattt cttgccgggg cgatctctgc ggtgctggga attattgtca cgatactgtc
     8521 atttcgtcgg ggctaacgaa ccagccagac cagcatcagg ataatcgcga ctaacagtat
     8581 ccacagcgcg ggacgcgttg ccatattttg caacgtgtcc atcatcggtg gaaaaggatt
     8641 aagcagcggt tgcatatcac gcggatcaat ccctttaacc cgccgttgat aaagctgatt
     8701 aagaatatcc tcggcctggg cagaagaaag cgatgattgc gcagaaaggc catatttttg
     8761 ctggatatat gccgataacg ccgccagttc actggcatct aaaggttgtt ttagcgccat
     8821 ctgtagtgat tccagcgtcg gcgtattttg ctggcttaac gtctggcgcg cctgcaacca
     8881 ggtcaccaga tggttaaaca gtttcgctgg aattaactcg ccatctttca ccccggaaag
     8941 ttccagcatc gattgccaga tctgtttgct gggttccccc gttgccgccg caagtttggt
     9001 caccagctgt ttgagcgcat tgtgctccgc cggtaataaa ggacggtcgg tcgcctcgcg
     9061 ctgctgcggt tgcggaataa ccatcttccc ttcctgcaac agggtgagaa tggtttttaa
     9121 ttgctccggt gagagctgat tcagcggcgt ctgaccaaag ttatgacgga tataatccgt
     9181 gaccgcctga cgattattcc ccagacgtaa atactcgcct aattgcgcta aaagctggcg
     9241 ggcagaatgg cttttttgcg cggccagcag acgttgcgcc agattatgtt cagcggcagg
     9301 gaagtgacgc gaaagcagcg gtgaatctcc cgacagacca atatcgtgcc tgatacccgc
     9361 ccacagttct gctctttgct gttgcgtcag tgaggtcact ttcgtcatta agctttccag
     9421 cgaagtacgt tgctgactgg ataaaggctg attgcccgcg ccagacggca ggttatctcc
     9481 ctgacctggt ggttgcccag gaggagggcc ggaaataggt tgtatcatta cgtatcctta
     9541 tacctgaaat cttcgcaagt atgcctggcc gcgagattat ggcacacttg tccggttaac
     9601 tctcgtctca tacaggtaac acaaacgtga aaatccttgt tgatgaaaaa aatgatgatt
     9661 taattaattg atataaaagg atttatgtca gagcgtgtcc acgcagtgac cacattttcg
     9721 agatgtaggg taagctcgca atattgcggg ctttgtctta agtatgatgc cctcacgtgt
     9781 gagggtgaca aaaagaaaac ccgcaatttt tacgctgcgg gtttgtcatt ctgattcgtc
     9841 cgttaggcat cagccatatt tgcgggcgaa atcttcatca gaggtgcata gatagataaa
     9901 aaactcaata aatgccacga ttgccggaat gaatgtccag caaaaaataa ggtaaaggaa
     9961 tccttgcccc actttaccta aataaaattt gtgtgcacca agaccaccta aaaagaaagc
    10021 cagtagtgcg gctgtcattc tgctttttga acttgctgtt ctttgaggtg ctccacaatt
    10081 ggggcaggat gtggcatctt tatgtatttc cttgccgcat ccgcgacaaa ataccatttc
    10141 actcataaag agttcctttc aaatttaaac aagtgttaag cccaccactc ttactggtgg
    10201 gcctcaattt aaatttgggt gccacttcgg ccttaccctg gcaaccgatt gacgggggat
    10261 tgctcccccg tcgcggtttc cttactgctt acactgtaag aacgccgcaa actccgctcc
    10321 ccagaagctc atccgtattt cgcacagcga accgtgtaac atccagatga tgaggattac
    10381 cgtcacgcag aacgtgatgg ccgtaagcga tttttgcgac atagcacttg ctccttttcc
    10441 ggagaggcgc taacctttca cttgcttagg gtagacggtt agggcctcgg ttaaacaaaa
    10501 gtgttttccg gggcctttcc acatctggcc ttcgggtgtt ccctccgacc atcagccgaa
    10561 aggcacccgc gcgtaatcta tcgctttttt gttactccgg caattctgcc tgttaattct
    10621 gaggtaaagg caaactcatc tgattgtttc ccctgtgtga agctggcagc tcatgccacg
    10681 ggataccttc tgaagagtga acgccggagg cttgtttcga tgtgaattta tggaaagctt
    10741 ccagtgttga gaagcatacg ccgcattcca gattgttaca ctggtaatac ttttgccgca
    10801 cggtgtttga atcattttcc ggacgacttg tgcggatacg ggcagatgcg ccacaaagcg
    10861 gacaacggaa catagcgacc tcccttaacg tggtgctgcc gctattctag attgtacgat
    10921 ccctaattcc aactaacagc tcgctctgtg cgaagagtga aaattgaact ttcaatagct
    10981 gttgaacttc catacatcca atgtaaaaat tcatacgttc atgatagtta aaatgtaaaa
    11041 tattgtatgt caggtgaata aagcatggga agagatataa cgctgtaccc taaaaaagca
    11101 actagaaacg aacttaaaaa ttatcttgaa aatttaggtt ttaaaaggtg taaacatttg
    11161 tgggaatggc cccaaggaac gttaaattac tcttggtttg atgatacgga cttcaaatct
    11221 acagatggag tttcggctga tatatatcct gtttcaaatg acgaactaca catatctggt
    11281 aataaatggg cattgcatgt gcgtaattta tctagcgcta gttggcatga tgtaaaaatg
    11341 cttaatgatg ttctaaaagg cgcaaggagt ctttttggtg gaacacttat aggtgattat
    11401 gggaaaaata gatacgctcc tctatgggag gattctagta gtccgatcag tcgtggcatt
    11461 tcttcaatat ttaatcatgt tcatcatgag atttccgctg ttaaacatgc cttacctgaa
    11521 ccttctatta aattaaacct gccggaagat ggcggcttat ccgaatactt tgatgatatg
    11581 caatgtatgg atcccagtag agttatatat aatggattag taccttttgc ggttgctatg
    11641 tttgaatatt ttttctctag agcttttcaa atacttataa agtacgatcc tttcgcaata
    11701 gcaaaaagaa catcgtataa gcaaaaagtt gattttgata ttctgcttga gatagagaaa
    11761 ggaaatattt caatagaaag tgttattgca agaaattaca cttttcaaaa cttaaccctc
    11821 ctaaacaaag catataaaga gtggctagat atagatgtga gagcgattct ttataagaaa
    11881 aaaagaattg gaaaatcagt tgattacctt gagaatcgaa tttctgaaat catacaatat
    11941 agacatggga tagttcatca ttttgagttg gataggacct taaatcggga tggatatatt
    12001 catatattag acgcgataga aaaaagcata attgaattta tccattacat agaaagcaaa
    12061 tacaaattta aattaaatgc gtattaagca atcaacctta catggggatg acaaagtccg
    12121 cttctggcac ggagcggacc gtcgtttatg tttaattcta tactgtaaac ttgccagctt
    12181 aagtctgagc taatatatct aagttgctca ctctgtttct gctatccatt ccgggatttt
    12241 tgcttcaagc tcaagctgcg tggtaaagcc gctgttatca atggtgtgct cggcttttgc
    12301 aataatccag tcctgattgt cgatgtcact tttgaagcct gtcaccgtgc catgcatttc
    12361 ggggtagagt tccgcacgtc cacgcgccag cgtgatggag aatgatgcag ctccgcgttg
    12421 tagctgctgc cactttgccg ccgctgcgcg tcttgctgcc tgctcgttct gataagtctt
    12481 gcgtaacaca aacacattgc cttccgcgcc ttccatataa tcaccttcac ggctgctgct
    12541 tttctccttt ttgggttttg gcggtttgcg gcgtttcacg ctgacttttt tctttttccc
    12601 gtaattaaga tcaagccagt aagcgcgtac ccccgtatac gcctcgcggt cagcaatgcg
    12661 gaactgatgg cgatcgccgc tgctgcgtgt aatggcgaac gagggcaacg gctggccctg
    12721 cgcgttcacg ccaccacctg gcatgatgaa taacagattg ccgcttttta ccgtggtgat
    12781 tgcgcccagc atttccgcca tgcgcgtaag gaaggacatg tcgctttctt cggtctggtc
    12841 ggcgtggtcg atttcgatat ccatcagcat ttcgctgatt tgcggtttca gaccataccg
    12901 atgagcgatg gcggatacca cacgctcaac ggtcacatca tgccaggaca cctcacgttt
    12961 aacgttaaat tcatcccgaa aatctgcgct tctggctgaa acagtcagcc tgtccggcgg
    13021 tccttcgtga gcgatttcat caacaatgta agtgcctttt tctgtcagcg gttctccttt
    13081 ccagccaatg agaaccgtca ggcgcgcgcc ccgtggcggt agctgcaact gaccatccgc
    13141 atcatccagc gtgatggtga gctggtccgc ctcaaatccc cggttgtcgg tcagtgacag
    13201 gctcatcagg cgctctgcca cgcctgacag cgttttaccc tccgcgagaa tatcaaaatc
    13261 cggcattttc acgggggctg tgccctgact gagcaattgc atggtggtgt cggtcatctg
    13321 ttccctccct gtgcggcatg gtcgcatgtg cgtgcggagg gggttactgc tttttgttgt
    13381 cgccgtggcg ggagaatagc gcaggggtga gattacgcgc gtggtgggtg atgattgttg
    13441 ctgaatcatt taacggatac aaggggctga agctatgagt gaaactcgtt ttcatggtgc
    13501 ccgtgttacg gaaaataccg acctggtaac agcgattaac gatgttgatt ccagcgttat
    13561 cggtatcgtg gcaacggcgg atgatgcgga cgcgaagctg ttcccgctga acaagcccac
    13621 actgctgacc cgcgtcaatg acgtgctggg aaaatgcgga acaacgggga cgctttatcg
    13681 tgcgcttaag gccatctcag accaggtgag cacaaaggtg atcgtcgttc gcgtggctga
    13741 acacaaagaa gaagacggaa agacgcagga tcaact
//
LOCUS       DAFATA010000114        13408 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFATA010000114 DAFATA010000000
VERSION     DAFATA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.115"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1063)
                     /gene="waaA"
                     /locus_tag="LGJ20_003999"
     CDS             complement(<1..1063)
                     /gene="waaA"
                     /locus_tag="LGJ20_003999"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBK2388005.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFN"
     gene            1476..2534
                     /gene="rfaQ"
                     /locus_tag="LGJ20_004000"
     CDS             1476..2534
                     /gene="rfaQ"
                     /locus_tag="LGJ20_004000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBK2388006.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            2531..3655
                     /locus_tag="LGJ20_004001"
     CDS             2531..3655
                     /locus_tag="LGJ20_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK2388007.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            3648..4445
                     /gene="rfaP"
                     /locus_tag="LGJ20_004002"
     CDS             3648..4445
                     /gene="rfaP"
                     /locus_tag="LGJ20_004002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBK2388008.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKLEQGLLSQAEAKATKIRERTIRKSL"
     gene            4461..5477
                     /gene="waaO"
                     /locus_tag="LGJ20_004003"
                     /gene_synonym="rfaI"
     CDS             4461..5477
                     /gene="waaO"
                     /locus_tag="LGJ20_004003"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBK2388009.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            5494..6489
                     /locus_tag="LGJ20_004004"
     CDS             5494..6489
                     /locus_tag="LGJ20_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HBK2388010.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            6499..7191
                     /gene="rfaY"
                     /locus_tag="LGJ20_004005"
     CDS             6499..7191
                     /gene="rfaY"
                     /locus_tag="LGJ20_004005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBK2388011.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKTEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            7217..8242
                     /locus_tag="LGJ20_004006"
     CDS             7217..8242
                     /locus_tag="LGJ20_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HBK2388012.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            8327..9310
                     /locus_tag="LGJ20_004007"
     CDS             8327..9310
                     /locus_tag="LGJ20_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK2388013.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNDKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            9356..10609
                     /locus_tag="LGJ20_004008"
     CDS             9356..10609
                     /locus_tag="LGJ20_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK2388014.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFITIILLCAYFKAQSDQCLLEK"
     gene            complement(10679..11650)
                     /gene="rfaC"
                     /locus_tag="LGJ20_004009"
     CDS             complement(10679..11650)
                     /gene="rfaC"
                     /locus_tag="LGJ20_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBK2388015.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(11654..12700)
                     /gene="rfaF"
                     /locus_tag="LGJ20_004010"
     CDS             complement(11654..12700)
                     /gene="rfaF"
                     /locus_tag="LGJ20_004010"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBK2388016.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEV"
     gene            complement(12710..>13408)
                     /gene="rfaD"
                     /locus_tag="LGJ20_004011"
     CDS             complement(12710..>13408)
                     /gene="rfaD"
                     /locus_tag="LGJ20_004011"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBK2388017.1"
                     /translation="SSTTEWDGKYMMDNNYQYSKELLHYCLEREIPFLYASSAATYGG
                     RTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANSQIVGFRYFNVYGPREGHKGS
                     MASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVADVNLWFLENGVSGIFNLGTG
                     RAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFTQADLTNLRAAGYDKPFKTVA
                     EGVTEYMAWLNRDA"
BASE COUNT         3789 a         2799 c         2970 g         3850 t
ORIGIN      
        1 agttaaaagt atgcggcccc atcaataccg gaatagcgtg tgcggcagct tccagcggat
       61 tatgcccacc acgttcaacc agtgaaccgc caacaaaggc gagatcggca atgccgtaca
      121 gtaacatcaa ctcgcccatc gtatcgccaa ccacaacctg cgtgctggtg gaggggactt
      181 cccctgaaga gcgtgtgata tagcttagtc cagcctggcg gacaagatta atcgcatccg
      241 ggaagcgttc tggatgacgg ggtaccagga tgagcaataa attcgggaat tgctgtaaca
      301 atgcctgatg tgcggcgatc accacactct cttcgccttc gtgagtgctg gtggcaatcc
      361 ataccgggcg gtgtggtgcc cactggcggc gcagcgtcac ggctttagca gccaactgcg
      421 gcgttacaga aatatcgaat ttcaggctac cggtaacggt cacctgatta ttttttgcgc
      481 ccagcgccac aaaacgtgca ccatcttctt cattttgcgc agcaatcagc gtaatacgac
      541 gcagcaagcg acggacgaat ttacccagtt tggcataacc tgcggccgag cgggcagaga
      601 gtcgcgcgtt agcgatcacc agcggaattt tacgtttgtg tagcgccgca atcaggttag
      661 gccatagttc ggtttccata atcaacacca gtttggggtc gactttattc aggaaacggt
      721 tgagcgcatc gggcaggtca tacggcagat aaacgtgctg aacatccttc ccgaaagccg
      781 attgtacgcg ctccgaaccg gttggcgtca tggttgtaac ggtaatcggt aaatcaggat
      841 aacgatggcg cagcgcgcgc accaacggga tcgccgccag agtttcaccg acggagacgg
      901 agtgcagcat aatgccgcct ggttttagcg gatggcggta aaaaccgtaa cgttcacccc
      961 agcgttttcg ataggccgga gccttacgtc cgcgcaccca gagccgtatc cagatcagcg
     1021 gctgaataag gtagagaagg gcggtgtaaa gcaattcgag catagtaaat agctgactta
     1081 tggatgtgct ggggattcta tgtatttagc tgtggcttta ccattacttt tcccgttttt
     1141 gacttaaata gcaatttacc cgccaattct tgctgaacat ctatactttt tttgtatttt
     1201 tatgcgattc attgatttct gctgtacgtg caggaaggaa tcgcagagca acagtgaaag
     1261 aatatggttg ctaatgattt tatttgattg atttctttat tgaaaatcaa caatgtgctt
     1321 aaaaatcatt cgggtcggat tgccgcgaaa gaagtgatac actagcgcgt caatatcagt
     1381 gcggtatcag tattcaggta gctgttgagc ctggggcggt agcgtgcttt ttctgcttca
     1441 ccttaatcgg ataatctcaa caaaaagagt tacttgtgga taagccattt cgaagaattt
     1501 tgctcattaa gatgcgtttt catggggata tgttattaac tactcccgtc attagttcgc
     1561 tgaaaaaaaa ttaccctgac gcaaaaatcg atgtgctgct ttatcaggac accatcccga
     1621 tcctgtctga aaatccagag attaacgcgc tctacggcat aaaaaataaa aaagcaaaag
     1681 cctcagaaaa aattgccaac ttttttcatc tcatcaaggt attacgtgcc aataagtatg
     1741 accttatcgt caatcttacc gatcaatgga tggttgctat actggttcgc ttattaaatg
     1801 cccgtgtgaa aatttcccag gattatcatc atcggcagtc tgctttttgg cgtaaaagtt
     1861 tcacccattt ggtgccgttg cagggtggaa atgtggtgga aagtaactta tccgtgctga
     1921 caccattggg acttgattcg ttggtgaagc agacaaccat gagttacccg cctgcaagct
     1981 ggaaacgtat gcgtcgcgaa cttgatcacg ctggtgttgg acaaaattat gtggttatcc
     2041 aacctacggc gcggcaaatc ttcaaatgct gggacaacgc caagttttcc gctgtgattg
     2101 atgccttaca tgctcgtggt tatgaagtcg ttctgacgtc cggcccggat aaagacgatc
     2161 tggcctgcgt caatgaaatt gcgcagggat gccagacgcc accagtaacg gcgctggctg
     2221 gaaaggtgac cttcccggaa cttggtgcgt taatcgatca tgcgcagctg tttattggcg
     2281 ttgattccgc accggcgcat attgccgctg cagttaatac gccgctgata tcgctgtttg
     2341 gtgcgacaga ccatattttc tggcgtccct ggtcaaataa catgattcaa ttctgggcgg
     2401 gagattaccg ggaaatgcca acgcgcgatc agcgtgaccg aaatgagatg tatctttcgg
     2461 ttattccggc ggcagatgtc attgctgctg tcgataaatt actgccctcc tccacgacag
     2521 gtacgtcgtt atgatcgttg ctttttgttt atataaatat tttccctttg gcggtttgca
     2581 gcgtgatttt atgcgtattg ctcagacagt cgccgcccga ggtcatcatg ttcgggttta
     2641 tacccagtcg tgggaaggcg aatgccctga tgtatttgaa ctgatcaaag tgccggttaa
     2701 atcgcatacc aatcacgggc gcaatgcgga gtattttgcc tgggtgcaaa aacatttacg
     2761 cgaacatccc gtcgataaag tcgttggatt caacaaaatg ccggggctgg acgtttatta
     2821 tgccgctgat gtttgttatg ccgagaaagt agcgcaggaa aaaggctttt tctatcgcct
     2881 gacgtcacgt tatcgccatt atgccgcctt tgagcgggca accttcgaac agggcaagcc
     2941 gacacagctg ctgatgctga cagataagca aatcgccgat ttccagaaac attatcagac
     3001 tgaagcggag cgttttcata ttctgccacc ggggatttat cctgatcgta aatatagcca
     3061 gcagccagcc aatagccgtg aaatcttccg taagaagaat ggaataaccg aacaacaata
     3121 tttattgttg caggtcggtt cagacttcac gcgtaaaggt gtcgatcgtt ccattgaagc
     3181 acttgcttcg ttaccggatt cgctgcgcca caacacattg ctatatgttg ttgggcagga
     3241 taaaccgcga aaatttgagg cactggcaga aaaacgcggc gtgcgcagta atgttcactt
     3301 cttctcgggg cgcaacgatg tctcggaatt aatggcggcg gcggatttat tactgcatcc
     3361 tgcctaccag gaagcggcgg gaattgtgct gctggaagcg attactgcag gattaccggt
     3421 actaacaact gccgtttgtg gctatgcgca ttatattgtc gacgctaatt gcggcgaggc
     3481 tattgctgag ccattccgcc aggaaacatt gaatgagatt ttacgcaaag cgttaacgca
     3541 atcttcattg cgccaggctt gggcggaaaa tgcgcgacat tatgctgata cacaagattt
     3601 atacagtctg ccagagaaag cggcggatat cataacgggt ggtctggatg gttgaactta
     3661 aagagccgtt tgccacgtta tggcgcggca aagatccttt tgaggaagtt aaaaccttgc
     3721 agggtgaggt atttcgtgaa ctggaaactc gccgtactct gcgctttgaa atggcgggca
     3781 aaagctattt tctcaaatgg catcgcggca cgaccctgaa agagataatc aaaaatttac
     3841 tctcattgcg gatgccagta ttaggcgctg accgcgaatg gaatgcgatt catcgactgc
     3901 gggatgtcgg cgttgatact atgtatgggg tggcatttgg cgaaaaaggc atgaatccgc
     3961 tgaccagaac ttcatttatt attaccgaag atctgacacc aaccataagt ctggaagatt
     4021 actgtgctga ctgggcgacc aacccaccag atgttcgcgt aaagcgtatg cttattaagc
     4081 gtgtcgcgac gatggtgcgc gatatgcatg ctgcgggcat taatcaccgt gactgttata
     4141 tctgtcattt cctgctgcac ttgccttttt ccggtaagga agaggagtta aaaatttcgg
     4201 taattgacct gcaccgggcg cagcttcgca cgcgcgttcc acgtcgttgg cgcgataaag
     4261 atcttattgg gctttatttt tcttcgatga atatcggcct gactcagcgg gatatctggc
     4321 ggtttatgaa agtgtatttt gccgccccgc ttaaagacat tctcaagctg gaacaaggac
     4381 tgctgtcgca agcagaagca aaagccacaa aaatcaggga aagaacgatt cgaaaatcgt
     4441 tgtaatttcc ggagagaaat atgagtgccc actattttaa tccacaagag atgatcaata
     4501 agacaatcat cttcgatgaa aggccagcgg cgtcagtggc atcatcattc catgttgctt
     4561 atggcattga taaaaacttt ctttttggtt gtggtgtttc aatcacgtca gttttgttac
     4621 ataacaacga cgtgagtttt gttttccacg tttttattga tgatatccct gaagccgata
     4681 tccagcgttt agcccaattg gcgaaaagct atcgtacctg tatccagatc catctggtaa
     4741 attgtgaacg gcttaaggca ttaccgacga ccaaaaattg gtctattgcc atgtatttcc
     4801 gttttgtaat tgcagattac tttattgatc aacaagataa gatcctttac ctggatgctg
     4861 atatcgcctg tcagggaaac ttaaagccgc tgataacaat ggatcttgcc aataacgttg
     4921 ctgctgttgt tactgaacgc gatgctaact ggtggtcgtt acggggtcaa agtctgcagt
     4981 gtaatgaact tgaaaagggt tactttaatt caggtgtcct gttaattaat acactagcgt
     5041 gggcgcagga gtccgtttct gctaaagcga tgtcgatgct tgctgataaa gccatcgttt
     5101 cccgtttaac ctatatggat caagatatcc ttaatcttat cctgttaggg aaagttaaat
     5161 tcattgatgc taaatacaat acgcaattta gtttaaatta tgaattaaaa aaatcatttg
     5221 tttgtccaat taatgatgaa accgtattaa ttcattatgt cggcccgaca aaaccctggc
     5281 attactgggc cggttatcca agtgcgcaac cttttatcaa agccaaagaa gcatcgccct
     5341 ggaaaaatga accgttaatg cggccagtta actcaaacta tgctcgttat tgcgccaagc
     5401 ataattttaa acaaaacaaa ccaattaacg ggataatgaa ttatatttat tatttttatt
     5461 taaagataat aaaatgaaat tgagaaaagt taaatgaatg aatttataaa agaacggttt
     5521 tcgtatttag cagataataa aaaagaaaac gccccagagc taaatgtttc ctacggtatc
     5581 gataagaatt ttttgtatgg tgctggcgtt tcaatttctt ccgttttgat taataattca
     5641 gatattaatt ttgtctttca tgttttcact gattatgtgg atgatgatta tttaaagtca
     5701 tttaatgaaa cagcaaaaca atttaatacc tcaattattg tatatttaat tgaccccaaa
     5761 tactttgctg atctgccgac gtcacagttt tggtcgtacg cgacatactt cagggtattg
     5821 tcttttgaat atctgagtga aagtatttcc acactgctgt atctggatgc cgatgttgtt
     5881 tgtaaaggaa gcctgaaacc tctcacagaa attatattta aagatgagtt tgctgcggtc
     5941 attcctgaca atgatagtac tcaggcggca tgtgcaaaac gcctcaacat tcccgaaatg
     6001 aatggacgtt atttcaatgc aggcgttatc tatgtcaatc ttaaaaaatg gcatgaagca
     6061 aatttgacac cgtatttact caaactttta cgaggggaaa ctaaatatgg ctctcttaaa
     6121 tatttagatc aggatgcgtt gaatatcgca tttaatatga ataatatcta ccttgcgaag
     6181 gattttgata ctatttatac cctgaaaaac gaactttatg atcgtagtca tcgaaagtat
     6241 cagcaaacca ttaccgataa aacagtgttg attcactata cagggataac taaaccatgg
     6301 catagctggg ctggatatcc gtctgcatca tactttaata tcgcgcgtga acaatctccc
     6361 tggaagaaat atcctcttaa agaggcgcgg actgttgcag aaatgcagaa acaatataag
     6421 catctgtttg cccatggtga gtatataaaa ggcataactt cattaattaa gtacaagctt
     6481 aagaaataaa ttactcttat gattacaagt atacgttatc gcggcttctc attttattac
     6541 aaagataatg ataataaata taaagaaatc tttgatgaaa tactggccta taattttaaa
     6601 acagtaaaag tattacgtaa tattgatgat accaaagtat cattgattga tacgaaatat
     6661 ggtcgctatg tttttaaagt ctttgccccg aaaacgaaaa ggaatgaacg ttttttaaaa
     6721 tcttttgtaa aaggtgacta ctatcagaat ttgatcgttg aaaccgatcg tgtcagaagt
     6781 gcaggtctta cttttcctaa tgatttttat tttctggctg agcgtaaaat tttcaactat
     6841 gccagtgttt ttattatgct tatcgagtat gtcgagggcg tagagctcaa tgatatgccg
     6901 attattccag aaaatgttaa aacagaaatt aaggcttcaa tggaaaaatt gcatgcctta
     6961 aatatgctat cgggcgaccc acaccggggt aattttatcg ttagtaaaga tggtgtcagg
     7021 ataattgacc tatccggtaa aagctgtacg gcagagcgta aagctcgtga tcgtctggcc
     7081 atggaacgtc acctgggtat tgccaacgaa ataaaagatt atggctacta ttcagtaatc
     7141 tacagaacca aactacgcaa atttattaaa aaattaaaag gcaaagcgta aaccacacag
     7201 tcaaaacgga accaacatgg atttattagc tgagagtatt actgaagtcg ctgtctctgg
     7261 ggaaattgct aacaccgatc gtgtgttaaa tatcgcttac ggtattgacc gtaacttttt
     7321 atttggtgcg gcagtatcta tgcaatcagt tgttatgcat aacccggacc ttgcggttaa
     7381 gtttcatctc tttaccgact acattgatga agattatcta caacgtgtta atgcttttac
     7441 cagcaaaaat gctaacgttg aagtaataat ttataaagtc tccaatgcct ttattgatat
     7501 cttccccagc ctgaaacagt ggtcttatgc aacattcttc cgtttagttg cgttccagta
     7561 tttgagtgaa actattgaaa atctgttata tatcgatgct gatgtcatct gtaaaggctc
     7621 attagctgga ttgcttgata ttaattttga tgaggataag ttcgcagctg ttattaaaga
     7681 tgtgcctttt atgcaggaaa aaccagcgaa gcgtctggct atagagggac ttccagggaa
     7741 ttatttcaac gccggtgtag tatatctgca gcttgaagca tgggcgaaaa atgattttat
     7801 gaataaagcc attgctatgc tggcaagtga cccgcagcac acgaagtata aatgccttga
     7861 tcaggatatt ttaaatattc tgttctttgg tcattgtatt tttattagcg gcgattatga
     7921 ttgcttttat ggcattgact atgagttaaa aaataaaagc gatgaagatt ataaaaagac
     7981 cattaccgat gatactaagc tgattcatta tgttggcgta acgaagccct ggaacgactg
     8041 gacgaattat ccctgccaga agtattttaa tgaggcttat caggcttctt gctggaatga
     8101 tgtggcgttt attccagcca cgaatgaaaa gcagtatcaa gtgaaatatc aacatgcaaa
     8161 gaaaaatggt gatacgttta acgcttttat ttacttcatt aaatttaaat taaataagta
     8221 taaaagaaaa ctatttggat aataataaaa atcatggatg tctgagttac aataataatt
     8281 aaggatgagt actatcatat tttattagtt caggcaagag aacacgatga gcaatgatta
     8341 ccctttagta tccataataa taccgacgta taattcatct gattacatta ctgaaactct
     8401 aacgaaatta gaaaaacaaa cttacccaaa ttttgaaatt gttattgtta atgatggttc
     8461 taaagataac acatcaaacg ttttgagaga gtatgggtta acccactctc gattaattat
     8521 tatcaataaa gaaaatggcg gtgtttcgtc tgccaggaat acaggtatcc gcaaggcgca
     8581 aggacagttt atatgtttta tggatgatga tgatgagata gatcctaact atctgctgaa
     8641 gatgtatacc agacaacatg agacgggagg ggatgcgatt tattgtgggc tttatggcca
     8701 ttatataaaa gatggtgtta cttactcacc tataaataca gagtttaatg aaggatcttt
     8761 acttttcgac tttttttata aaaaggttag attccatata gggtgcttgt ttataagaaa
     8821 acaacttctg gaagagaata atcttttttt tgatgaagat ttacgactag gagaagatct
     8881 tgattttatc tatcgactgc taattacatg cgatatgtat gcggttccat attatatgta
     8941 taagcataac tatagagaaa attccttaat gaactcatgt agaaccatca ctcattatcg
     9001 acatgagtca tttgcgcacg aaagaatcta ctcttctgtg atgcagttat acaaaggtaa
     9061 ccggaaagaa gaaattcata cattattgag tcaaaataga gcttatcata aaactcgtta
     9121 tttatggaat gttctactta atggtgattt taaactattg aatcaattag ttgaaagcaa
     9181 tgataaagaa ttaagagatt gtaatcttcc tggcaagaga gataagagac gagcaaaaat
     9241 attagcatca aaaaattata ttctctggag gatggtaaga ctggtaaata gaaaaaagaa
     9301 taaacgttag atcttgcatt aaataaccca tcctcgtagc ataggttgaa actatatgtc
     9361 gttttgttgg aatgaaatta attctggtat aaagtcttta attctcatat tatgtatttt
     9421 ttctttaatg actttgtctt tatgggatga tgttgcaaca aagtttcttc atgcagctgg
     9481 aattatatct gcattgtatt ttcttgcgac accaaaaaaa acaataacta ataatcctac
     9541 tttgttaatt ttcatctcat tatgtctttt gggtatcgta aatatcatct ggtattcaca
     9601 ttataaagtt tcaggctctg tttataccaa tgcatatcgt ggcccaatgg aaactggaaa
     9661 aattgccttg tgtagcgctt ttattttctt agttcttttt gctaaaaatg agatgagaac
     9721 aaaaataaaa tttgggaaac taattctgtt cgcatccctg gcaacgcagt tacttttttt
     9781 tgcgcatgcc atgtggcaac atttctattt aaacgtcgac cgtgttgcat tatcagcttc
     9841 ccacgctaca acagcaggct acatcatcct ttttccttct ttactggcat caattctcat
     9901 tttaaaatcc gacttaagac ataaaacaac attatataca attaacttca tgcttagttt
     9961 atgtgctgtc atagtaactg agacgcgtgc agccatatta gtgtttccat tctttgcgtt
    10021 aatattaatc gtaatggata gttatattaa taagcgaatt aattataagt tatattgttt
    10081 tattacgatt gcattattag caggtgtatt ttcttttaaa gatacattgc ttatgagaat
    10141 gaatgactta aataacgatt tagttaatta ttcgcatgat aacaccagaa cttcagtcgg
    10201 tgcccgtctg gcaatgtatg aagttggctt aaaaacatat tctccaatag gacaatcact
    10261 ggaaaaacgt gcggaaaaaa tacatgaact agaagaaaaa gagcctagat tgagtggcgc
    10321 tttaccctat gtagattctc atttgcataa cgatctcata gatacgttat caacgcgtgg
    10381 tattcctgga gttgtattaa caattttagc attttcagca atactcatat atgccttaag
    10441 aactgctaaa gaaccttata ttttaatctt gcttttttca ctactggtag taggactaag
    10501 tgatgtaata ctcttttcta aaccggttcc gactgctgtg tttatcacca taatattgct
    10561 ttgtgcttat tttaaagcac aatcggacca atgtttatta gagaagtaat gaatatgtga
    10621 aataaaatca gcaaggaagc tgattttatt tcaaaattat ctattaaaga gttgtaattt
    10681 aaatcatggc agcgttttct tcaatgaacc gcttaacagc atttgctgtc aattgagaca
    10741 actcattccc cggagcccta caaaccatct gattcttccc atacccacca attaatcccg
    10801 gatcggtagg tccataaacc gtgatattgg gtctatccag tgccgctgtt aaatggctta
    10861 accccgtatc cactgacact acaaatttag ccccggccag cacgcgggca acgccttcca
    10921 gactcatctt cggcaatact tcaacataag caaatccttc cgccagtcgt ttcgcccgtt
    10981 cttcctcatg cggcgcgccc cacggaagtt taatccgtat tcctgaatca gccagtaaac
    11041 caatcaattc tcgccagtgt tcttccggcc agtgtttatc atcacgggtc gtcgcatgaa
    11101 gaaatacggc atattcgcca gcatctgtag gcaggttcgt caggaaatgc tgtgcaatag
    11161 cataatcgcc ctgggtttgc ggtttgctat agcccagact tttggcaaac agttcgcggg
    11221 tgcgttctac ggcgtgctgc tgttttgcaa tatgatgctt acgattgtaa aacaggctgg
    11281 ctaacggttc gcgagcggtt tgccagtcca atccatgctt tacgccatgc gccagacgcg
    11341 tcaccagcgc cgcgcttttt accagcccct gagcatcgat aactgcgtca tagttctctg
    11401 cttgtagcgc ttcacgaaac gctttgcgtt cagcttttat gggggccgag aaccaggctt
    11461 tacgccagcg acgtattgcc acaggaataa ctcgctcaac ggcagcgtgc caggaaggaa
    11521 tctgtgcgaa cccttcttcc accacccagt caaacttaat ccctgggatt gcctgctggg
    11581 catcagtgag tgcgggcaac gtatggagaa catcgcccat cgacgatgtt ttaacgatca
    11641 aaacccgcat ccgtcagact tcctcttgta acaatagcgc gttgagttct tccagtacgc
    11701 gctggggagt aatgtcgatc aagctctggt gataaccctc cgcagcgtca cctttacgca
    11761 ctttgtgata gccggtaatc agacggatca cgcgcgcttt atgggatagc ggcggtgtga
    11821 agtccgggct acttggacca tacaaggcaa ccagcggacg attgagcgcc gccgcaacgt
    11881 gcatcaggcc agaatcgtta gtgacaatgg ctttacaggc tgcaatcagg ataaccgctt
    11941 gatcaagctg tgtttccccc gccaggttcc gacaccatgc ctgctgctcg gtattcaaag
    12001 cggcaagaat ctcattgccc gcctcatgat ctttcgccga gccaaacaga accacctgat
    12061 aaccttcatc aatcagctgc tttgccagct cagcatagtg gtagtgtggc cagcgttttg
    12121 ccggaccaaa ctccgccccc gggcaaaaac caatcatcgg acgttctgat gaaagcgaaa
    12181 attgattaca ggtatatgat ttttcacctt cgctcacctg caactgcggc cataacaatg
    12241 gctgcggcag atcttgtgcg gtacgcatca cgcctttgtc ataggccagc gcgacatagc
    12301 gttccaccat tagcggccag gcttctttat cgagcacgcg tacatcgttg agtaaaccgt
    12361 agcgcatctc gccgcgccag ccggtgcgat gaggaatacc cgcgaagaaa ggcactaatg
    12421 cggatttgaa ggagttgggt aagacgtagg cgcggtcgta gcgcttttca cgcaggctat
    12481 gacccagttt gcggcgttcg ccgatttcca gcgctccgtg accgagaggc atagggatag
    12541 cttcgttaac ttccggcatc cgcgataata atggacggca ccatgccggt gccatcacat
    12601 cgattatcgc ctggggatag cgcgcctgga gcgtgcgata gagactttgc gacatcatca
    12661 tgtcgccaac ccaagacggg ccgatcacca gtattttcat gcagagctct tatgcgtcgc
    12721 gattcagcca ggccatgtat tccgttacac cttcagcaac ggttttgaat ggtttgtcgt
    12781 aacccgccgc gcgcagattt gtcagatctg cctgagtgaa cgcctggtag cggcctttca
    12841 gcttatccgg gaacggaatg tattcgatct ggcctttctt gtgataagcc agcgtagcat
    12901 ctgctactgc ctggaaggat tctgcacgac cggtgccgag gttgaagata ccggaaacgc
    12961 cattttccag gaaccacagg tttacatctg ccacgtcgcc cacatagacg aagtcgcgtt
    13021 tgaagttctc gctaccttca aacagcttcg gtgattcacc gttgttaagc tgagtgttga
    13081 gatggaacgc gacgctcgcc atgctgcctt tatggccttc acgcggtcca taaacgttga
    13141 aataacggaa gccaacaatc tgcgagttcg cttcaggcag gatttggcga acatattcat
    13201 caaacaggaa ttttgagtaa ccgtagacgt tcaacggttt ttcgtactcg cgggattcaa
    13261 taaagtcgga ggtgcgtccg ccgtaggtgg ctgcggaaga agcgtacagg aacgggattt
    13321 cgcgctccag gcagtagtgc agcagctcct tggagtattg atagttgtta tccatcatat
    13381 acttgccgtc ccactcggtg gtggaaga
//
LOCUS       DAFATA010000115        13052 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFATA010000115 DAFATA010000000
VERSION     DAFATA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.116"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..185)
                     /locus_tag="LGJ20_004012"
     CDS             complement(<1..185)
                     /locus_tag="LGJ20_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBK2388018.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEAS"
     gene            555..1319
                     /locus_tag="LGJ20_004013"
     CDS             555..1319
                     /locus_tag="LGJ20_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBK2388019.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(1466..3628)
                     /gene="uvrD"
                     /locus_tag="LGJ20_004014"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1466..3628)
                     /gene="uvrD"
                     /locus_tag="LGJ20_004014"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBK2388020.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(3712..4428)
                     /gene="yigB"
                     /locus_tag="LGJ20_004015"
     CDS             complement(3712..4428)
                     /gene="yigB"
                     /locus_tag="LGJ20_004015"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBK2388021.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4428..5324)
                     /gene="xerC"
                     /locus_tag="LGJ20_004016"
     CDS             complement(4428..5324)
                     /gene="xerC"
                     /locus_tag="LGJ20_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK2388022.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5321..6028)
                     /locus_tag="LGJ20_004017"
     CDS             complement(5321..6028)
                     /locus_tag="LGJ20_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBK2388023.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6025..6849)
                     /gene="dapF"
                     /locus_tag="LGJ20_004018"
     CDS             complement(6025..6849)
                     /gene="dapF"
                     /locus_tag="LGJ20_004018"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK2388024.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6886..7089)
                     /locus_tag="LGJ20_004019"
     CDS             complement(6886..7089)
                     /locus_tag="LGJ20_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2388025.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7439..7993
                     /locus_tag="LGJ20_004020"
     CDS             7439..7993
                     /locus_tag="LGJ20_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HBK2388026.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            8361..9839
                     /locus_tag="LGJ20_004021"
     CDS             8361..9839
                     /locus_tag="LGJ20_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBK2388027.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYEGAQAWYPHYS
                     WHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKRTDNNGGGNRL
                     DIKTLHQVEFRPPRDPDLLGLILRDANFLYINGHRLADPDSFRVLAQKSWDQRGKFST
                     AFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFQPISDAVAQ
                     YQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHV
                     FESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLWRYRTADPKQV
                     RLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            9823..11148
                     /locus_tag="LGJ20_004022"
     CDS             9823..11148
                     /locus_tag="LGJ20_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBK2388028.1"
                     /translation="MRRQNEGSNNQVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGK
                     GYWGDKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMYRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSIFGEDQLEIIP
                     LDTPDLVIDRSFNGGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            11196..11516
                     /gene="cyaY"
                     /locus_tag="LGJ20_004023"
     CDS             11196..11516
                     /gene="cyaY"
                     /locus_tag="LGJ20_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBK2388029.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(11556..>13052)
                     /locus_tag="LGJ20_004024"
     CDS             complement(11556..>13052)
                     /locus_tag="LGJ20_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBK2388030.1"
                     /translation="LSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQSY
                     RNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLTFI
                     YVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLYIK
                     GNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAFRN
                     QVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTILGK
                     MHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVSGQ
                     TWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEGII
                     QFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGSSF
                     INFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
BASE COUNT         3161 a         3405 c         3371 g         3115 t
ORIGIN      
        1 gatgcttcga tgtcttccag ttccgggcgg gttgccaggc tctggccaag ttcagattgt
       61 acgcgcagtc gctcgtcgtc gtccggttcg acaagatcaa tccatactgc atttacaagg
      121 ggttgtgact cttcgacttc cagccgggtc agtcggttat tttccagttg aaatgcgctc
      181 agcatgaccg ggactcccaa tgcttaaaat atcggacagt tcggtgggca cacagaaaca
      241 aattgggttt cagaccatta aacagcctga ctcagcgcga cgggaaaaat tgaggtcgct
      301 gacaaccgct aaggctatca gcaaaaaggg atagccttag gagttgatcc tggatgacag
      361 gatagtgagc cagtatctac tgggtgtgtc caaggcgaat gtcctcttag agtgatcgtg
      421 cgcgcatgtt acgccagcaa aattttgccg tcaacacgca acagaacacc acagaacaat
      481 agttgaactt cgcgcttttt ggtactcgtc aaggtcgcta acaggataaa aaaatccaac
      541 atccaggacc tataatggcg catcggctac tcattggtaa aggaatgatc accttgaatc
      601 tcaaacggat ttttctcgcc ctcaccttac ttcccttgtt tgctgttgcc gctgatgatt
      661 gcgcactctc agatccgacg ctgaccgtac aggcgtatac cgttaatcct caaacagagc
      721 gggtgaaaat gtactggcaa aaagccaatg gcgaagcgtg gggaacttta catgctctgc
      781 tggcggatat gaatagtcag ggtcaggtgc agatggcgat gaacggcggc atctatgatg
      841 aaagctatgc gccgctcggt ttgtacatcg aaaacggtca gcagaaggtg gcgttaaatc
      901 tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg cgtgttttat gtcgcgggag
      961 ataaagtcgg catcgttcgt ctggatgcct tcaaaaccag taaagagatt cagtttgcgg
     1021 tgcagtcagg gccaatgttg atggaaaaca gtgtaattaa tccgcgtatt catcccaacg
     1081 tcgcctcacg taaaattcgt aacggtgttg ggattaataa acatgggaat gccgtgtttt
     1141 tgttgagcca gcaggcgaca aatttttatg attttgcctg ttatgccaaa gcgaaactga
     1201 acgttgagca gctgctttat cttgatggca cgatttcaca tatgtatatg aaaggcgggg
     1261 caatcccgtg gcaacgctat ccttttgtca ccatgatttc cgtagagcgg aaaggttaaa
     1321 acgatagttt tgtgtcagat actgaagatg gcgcagatgc cggatgcggc gtaaacgccc
     1381 tatccggcct acatgacgtt gcaatttatt gaatatgaat gattttttag gccaaataag
     1441 gtgcgcagca ccgcatccgg caacgttaca ccgactccag ccgggcgtat gccgccacca
     1501 gccatttaat accctggccc tggaatgcca cctgcaaacg gctatgctcg ccgctgcctt
     1561 ccatattgac gatggtgcct tcaccaaact tagcgtggcg tacacgctga ccgagcttgt
     1621 agccgctgtc gttctcgacc atcggcgtcc ccatccgttg atggctgacc ggacgactta
     1681 ccgtagcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg ataaagcgcg
     1741 acgggcgatg gtaaacctct ttaccataca gacggcgggt ttccgcgtag gtcagcgtca
     1801 gtttctgcat cgcgcgggtt acaccaacgt aggccagacg gcgttcttct tccagacgcc
     1861 cgccttcatc cagcgacatc tggcttggga acataccctc ttccataccg acaataaaca
     1921 cctgcgggaa ctccaggcct ttcgccgagt gcagcgtcat caactgcact gcatcctgcc
     1981 aggtatccgc ctgcccctcg cccgcttcca gcgccgcatg ggagaggaat gcctgcagcg
     2041 gcattaaatc ttcatcttct tcgttgtagc tgaactggcg cgttgccgtc accagttcct
     2101 ctaagttttc gatacgcgtc tggccttttt cgcccttctc ctgctcgtac atggtgcgca
     2161 ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg gcagtttctt
     2221 gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct cgcccggcga
     2281 gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg cgatcgcgcg
     2341 atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc gtattcacca
     2401 cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac gagagcgcgt
     2461 ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac ggcatactgg
     2521 cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag agaatggcgc
     2581 actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc acaaaacgcg
     2641 cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc gcgccatcgg
     2701 tccacagttt tttacccaga cgcccgttat tgttttcaat cagggcgtta gcggcgctca
     2761 gaatattgct ggtagagcgg tagttttgct ccagacgaat agtttcggca ccggggaaat
     2821 cattaaggaa acgctgaata ttctccacct gcgccccgcg ccagccgtag attgactggt
     2881 cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc cagcaggcgg atccacgcgt
     2941 actgaatgtt gttggtatcc tggaattcgt ccaccaggat attggtaaaa cgttcgcggt
     3001 agtgttgtag gatatgcggc ttgttaagcc acagttcgtg agcgcgcagc agcagctcgg
     3061 cgaagtccac caggcccgcg cggtcacacg cttcctgata cgcctgatac accttctgcc
     3121 aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg ccttcatctt
     3181 tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg tcgaggttca
     3241 tggctttgat cagacgctta agcaggcgca gctggtcctc gctatcgagg atctggaaat
     3301 cctgcggcag attggcgtcc atatggtgcg cacgcagcag gcggtgcgcc agcccgtgga
     3361 aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata cgatgacgca
     3421 tctccgccgc cgctttgttg gtaaacgtca ccgccataat cgagtatggc gagcagtttt
     3481 ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca ctgcccgcgc
     3541 ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt ttgtcattaa
     3601 ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag gttgggcgga
     3661 gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttgctg attatatcag
     3721 cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc tgtcccaggt
     3781 ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc tgcgaattgc
     3841 cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga tcggcacgtt
     3901 gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc cgtgcgggcc
     3961 agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct gggcgttacc
     4021 gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt cgtgagtttg
     4081 ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg cgtgtgcgcc
     4141 tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa tcgaacgaaa
     4201 acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg cctggcgcag
     4261 gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt gcacaaaggt
     4321 aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg tatcatccag
     4381 gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta tttcccccgt
     4441 ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc aagatgagta
     4501 tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc acgaagatcg
     4561 ccgctcgact ccagcatatg cgtagcgaac gagtgacgta atttatgtgg gtgaacgtga
     4621 ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt acgcgcggag
     4681 atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa caggtcgcgc
     4741 aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag gcggcgctct
     4801 ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg tttgatatcc
     4861 agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag cattgcacgg
     4921 tcgcgtacag cgaggggatc gttgatatca atatccagca gccgattcat atcgtcgacg
     4981 tcgatgtttt tcggcagatg acgcggcgct ttcggtgccg aaactccttt cgccgggtta
     5041 gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc agaaagccgt
     5101 aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa attgcgcacc
     5161 atcgtcacat cacattgctg ccagctttgc aggccgtttt cgctggcaaa attgatgatc
     5221 gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg ctccacgctc
     5281 agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt tcaatccaac
     5341 gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt ccttgccctt
     5401 gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca gcatcgcttc
     5461 ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc accagcagct
     5521 ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga atacgcagcg
     5581 gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca cctaagcgcc
     5641 agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc gcccagcggt
     5701 gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc tgcaggtaga
     5761 gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc gccatgttct
     5821 cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc aacgaaacgg
     5881 tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta cggataaaaa
     5941 actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc gtgagtgttt
     6001 cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga catgtaccgc
     6061 cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa gacgaccgcc
     6121 ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta ctgcaaccgc
     6181 cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat aaacgcgtaa
     6241 acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggctcgct ccggaaaacg
     6301 ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga catcatcgac
     6361 ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg tctgctcggc
     6421 ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg cggaaggttc
     6481 gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg tcagaaccat
     6541 ccgcccgttg gcggtgctga cgcggatatc gcgcttattg gtcagccctt tcagacgcac
     6601 aaaacgggca aagcagcgcg caccattgcc gcactgcgcc acttcactgc catcagcatt
     6661 gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca ccagcagttg
     6721 gtcaaaccct actcccaggt gccgatcagc caggcgacga atcagctccg gtgaaaaaaa
     6781 gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat gcattttcga
     6841 gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt aattcacctg
     6901 ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt gcgtttgcgt
     6961 ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat agagcggacc
     7021 tttcagaccg cagcccgtca ggctgaagag agtaattaat acagtgagtg ccttaaacac
     7081 gtttttcatt atggattgcc tgtaaagttc atcgctttct gctttctatc atcgcaggag
     7141 aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc tgaaaagtga ggaaatgctg
     7201 cgagtataac gcttacacgt ggggatttat gttttgctgg tagtttcgtg atcggcacct
     7261 cgcgccagga tggctttatg cactatcgca atgtggtttg tttgttgtct tgttcattgt
     7321 tcttatcgtc ggcatggggc ttttaataga gtggcgcacc atgcaatttc gttgtctgac
     7381 cttagtcgcc agtgacatac aactttgcca acctggtgat aagagggaat aaaatctgat
     7441 ggggctattt acccaactgg aaaaatttga ccaaaagctt acgcgcggtt atgcccgctg
     7501 ggggcgctgg gtatggcgga cgttaattgt cgttccggta ctggtcgtgt tgtggaatat
     7561 aggacaggca gtatggggag gaccaagagg tggagtgatt ctggaaatcc acagtgaaat
     7621 tgaccgtccg atactgggat tcagtgttaa cggcgtggcg ggcgcgaatg cttttgcaaa
     7681 tggtggcggt tcaatcacct gttgtggtga tgtcagtggg gatacggcag aggttatctg
     7741 gacattaagt actactcgta cacagtataa tgctggaatg cgtctggaga aacggaatat
     7801 gacgttacct ctccccaaaa gagaatgggg agaagatttt ctgcatgtgc attttatgcc
     7861 tggcgataaa gtgctgctgg ggtggagtaa agattctttt tctccgtatg aagatcttca
     7921 caacgggggg tacaagaccc gcgttcggca ggatgtgaaa gataaacttt atggtacagg
     7981 gaaaatgaat tgatagattt ctataaaaat cacgttcggc agaaagcgta ataatgtggg
     8041 gaatgttaga taccggtggt gggacctaag tacaacacgc gaaagacaat aaagtaggta
     8101 acggtgggca gagtcgcatg ttgacaccct tcccggcgtc aatccggcac attttaacgt
     8161 gatcagtcac accatggcgc agtagctcgt atttatatca tgaatgaaaa cctcaagttt
     8221 accccagcaa ctatcagaaa atagcgatgg cctttataga ttctgaaaag tgacgatttg
     8281 ccgcaaaagt tacgcttacg tgcgaggaat tatgttctgc tggtagtttc gtgatcggca
     8341 cctcgcgcca ggatggcttt atgcactatc gcaatgtggt ttgtttgttg tcttgttcat
     8401 tgtttttatc gtcggcatgg ggatgtcggc ttgatccgcc tgaacataat atttaccaga
     8461 aacagggaaa aggcgtggta tacctgcgcc cttataaaga aacaaatctt tctcttcctg
     8521 acgtgaatta caaaagtttg cgccgtttac ccaatttatt aatagacccg acaacactag
     8581 acaaatggga taaagtacct cctctgactg atctgacaac cgactacctc tatgaaggcg
     8641 cacaggcctg gtatcctcat tactcctggc atagcgatgg tcgctatatc ctctacgcgg
     8701 gtgaagttgt gcaaaacccg ccagacaaac ctcccgtcga tgtcgcctcg ttcaaggcgt
     8761 ggggtgattt tgccgcagat aaacacagtc tctatttcga aggcaagcgc acagataaca
     8821 atggcggggg aaatcgctta gatatcaaaa cactgcatca ggtggaattc cggcctcccc
     8881 gggatcctga cttactgggg ctgatattac gtgacgctaa tttcttgtat atcaatgggc
     8941 atcgcctcgc agatcccgat agtttccgtg tgctggcaca aaagtcatgg gatcagcgag
     9001 gaaagttttc taccgcgttt aatccctgtg tggctatgcc atttggcccc tgggataccc
     9061 tggctcgtac acggaccaaa atcctgatca acggcgaaca gcttgatgcc gacccggaca
     9121 ccttttccgt cgtgcgctgg atgcccggct cactcttgac ctggcgtgat aaaaacgggc
     9181 tgcagcgtaa agtcctcgac aaggaaaatc tggcgtggga tgaagattta acaaagcact
     9241 gtctggattt ttctctgctg gaaaagaaag tgttctggcg taaaggacct gcttgtaaac
     9301 aggaagaatt acccggactc gatccggagc agtttcaacc catcagtgat gctgtcgccc
     9361 agtatcagga ctcgctttat accatcatcg aaacagagtc tggtgaccgc aagctggaaa
     9421 tcgtgaaact tgatgatccc aatcttatta tcaacaaacg tttcaacgcc gggaaacgcc
     9481 acggctattt acttacgcgt gccgaagggt ggccatacca ttccggttta catgtgtttg
     9541 aatctgacgg accgctgatc ttactggata accgttctcc ggatgaacgc gaagcccatc
     9601 ttaatgacca tccctttttg cgcagatggt atgcccgcga taaccgctac atttacagct
     9661 ttgatggcgc gcagctctgg cgataccgca ccgctgatcc gaaacaagtt cgcttaatct
     9721 ggaaggaaca acattcggga tatggctatg gcgtaaatta caaaacggga tatctggacg
     9781 gaaaaattac tgatgacggc gaatttattc ctgccccgcg caatgaggcg acaaaatgaa
     9841 gggtcaaata atcaagttgc actcatcctg ttgggacaca catttctgac gcctgctgta
     9901 caggccatag ggttcgacta ttacaatgat cacggtgtta tgtcttacgg taaaggttat
     9961 tggggggata agaaaatcat tgcccagttt ccggagatga gcagggccga tttgcgcctt
    10021 gtgaaaaata tctctgggaa aacgggttat ataccttccg atgaaataag cataaaaaat
    10081 gaggaatacc actgggtgac agacggccgc gttattctct ggcgtggcaa aatcgttagc
    10141 aatccaccgg gaacccccac tgtcgatatt gccagctttc aggctatggg ccgcttcgcg
    10201 gtcgataaat atagcctcta ttttgacgga cagcgcaccg aaagtaatag cggcgcgtca
    10261 cgtgttgatc tggcgacact aaaagctatc gaaggtaact ctaccacgct ggtggatagt
    10321 aaaaatctct atttgtccgg tcgacgtcag gggagcagca gtaatgttac tgtattagaa
    10381 aaaagatggt ggggtattaa tccacgtctt atgagtgtaa acagaaattt gtattccaat
    10441 gatctgctta tccgcagtgg gcagaatatt tatttaaatg gcgttcacct tacggcgaat
    10501 gcagactcat ttgagataat tcgctggata cctcattcac tgctggtttt tcgcgacaat
    10561 aagggtatgt atcgttatcc ctttggtcaa ttatcaggca aagcgatacc cgtagatgat
    10621 gacgtctctt ttgaagtagg ggaaagtcgc gttcgctggc gtaaacagct cacgtccgac
    10681 cgtcagtgga gcaagtggat agacctacca ggtattgaac ctgaacaatt tcatctgatt
    10741 actggcaata ttgcgcaata taaagatcgg ctgtatgtaa caaaattatc gatatttggt
    10801 gaagaccagc ttgagataat cccgctggat acgccagacc tggtcattga tcgctcattt
    10861 aatggcggca aacaacatgc ttactttatc cgccaattac ggtcaaagag cttgcaaatt
    10921 attccagtta acggtccgct aactaaaaac gatcgcttcg cttatgacga tcgcaatgtt
    10981 tatacatgga ccgatacaga ggtaaggatt acgccctccc cctgcccggc gaaaactcgt
    11041 gtcagagagg agaacgtacg tgaagttcaa aacagagaca tcattattcc ggtgacggat
    11101 gaatcatgcc ggaacgcagc agcagaggtg caaactttga agccctgacc ccatgccgct
    11161 atactgccgc catcacttaa aaacaggata caacaatgaa cgacagtgaa tttcatcgcc
    11221 tggctgatca actgtggctg accattgaag agcgtctgga cgactgggat ggcgacagcg
    11281 atatcgactg cgaaatcaac ggcggcgtgc tgaccattac ctttgaaaac ggcagcaaaa
    11341 tcattatcaa ccgccaggaa ccgctgcacc aggtatggct ggcaaccaaa caaggcggct
    11401 accattttga tctgaaaggc gatgagtgga tttgcgatcg cagcggcgaa accttctggg
    11461 atttgctgga acaggcggcg acacagcagg cgggtgaaac agtcagtttc cgctaagatt
    11521 gcatgccgga taagcctcgc tttccggcac gttcatcacg aaaaatattg ctgtaatagc
    11581 ggcgtatcgt gatcctgatt ggcaggcggc atgttaccga tagattttgt gcggaacgga
    11641 atcacctgtt cacgaccatc aaccttcaca atctgataga actgcggcag gttgaagttg
    11701 atgaagcttg agccgtaggt gaagcggtcg tgcgatgacg agtagaagcg actgacgtca
    11761 cgcaccagtt cctctttgct gccttcgcag tggtgataca cctcaacccg gttgctttcg
    11821 tcgagaatgt agatattaaa gccattctcg tcttgcgttt cttcgaagaa gaactggatg
    11881 atcccttcac tagcaaagcc gtccaccacc gccggtaatt tgacgtgatt ggtttcaacc
    11941 tgcactgaca ggccgtgcag tttgttatgc gaaatagcgc cataaaactc gatggcgttc
    12001 tccagcttct gtaccgatac attcaggcgt tcgaagaaca agccccaggt ttgaccagaa
    12061 acgcgcagcg ccttgaaacg cccggtttcc tggcgggtgc tggaaaggcg cagttcaata
    12121 cactcagaaa ccagttgctg cacgcgagta cggattaacc cgcgcagatg ctggctataa
    12181 cagaagactt ccacgctatc tggcggtgcg gcgtcctgat gcattttgcc gagaatagtt
    12241 ttcagggctt cgatcatcga ttgctcgccg ttgaagtgca gcgtacgcac ttcgttccac
    12301 gagttgcggt acagcaggtc aacgctacct accaggcaat tttgattctc gccaaagctg
    12361 aagacatcca gcttacggaa atcaaaatgc accacctgat tgcggaacgc cgctgtcggg
    12421 tcatattcca ggttaacgat aatcgccaga tggcggatct cacacgggct gtagagcgcc
    12481 ttcggtgtcg gtgcaggtaa gcgcagcggg aaatggtgcg acacgtcggc gaccatctcc
    12541 tgcaacttag gcaaatcgac aatgccgtta cctttaatat acaaacgggt gcgcgaggtc
    12601 agcaggccgt taaaccatgc ccacgccacc agtttgttca ggtaacggtt atattccagc
    12661 ggctgatggc tgatgatcga ctcaatattt ggcgcgcggt tatacagata ccaacctgaa
    12721 cggttagcac ggcccggcgg cacataaata aaggtcagat tcggttccga gagatcgggt
    12781 gaaatctgcg ggtttaccag cgtcacttta cctggtaatg cttcaaacgc ggcatacagc
    12841 ttacgcgtca gcacgccgat atcctgcgga ctggcggaga cgctaaggtt attgcgacgc
    12901 gcaaagcgga tcagattacg gtagctctgc atcatcgcgt cgagcaactc gttgtgcgcc
    12961 tcacgcacct gatcaatctt ccagttagcg cggttatcga gcattgccag acgagcttcg
    13021 tcccaacccc actcgctcac taactggctc aa
//
LOCUS       DAFATA010000116        12891 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFATA010000116 DAFATA010000000
VERSION     DAFATA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.117"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..207
                     /locus_tag="LGJ20_004025"
     CDS             <1..207
                     /locus_tag="LGJ20_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBK2388031.1"
                     /translation="PMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRL
                     GAIFGVIYISMLLLVGWPILAMWN"
     gene            321..1127
                     /gene="rna"
                     /locus_tag="LGJ20_004026"
     CDS             321..1127
                     /gene="rna"
                     /locus_tag="LGJ20_004026"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBK2388032.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            1357..1767
                     /gene="rnk"
                     /locus_tag="LGJ20_004027"
     CDS             1357..1767
                     /gene="rnk"
                     /locus_tag="LGJ20_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBK2388033.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(1998..3236)
                     /locus_tag="LGJ20_004028"
     CDS             complement(1998..3236)
                     /locus_tag="LGJ20_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBK2388034.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            3457..3885
                     /gene="uspG"
                     /locus_tag="LGJ20_004029"
     CDS             3457..3885
                     /gene="uspG"
                     /locus_tag="LGJ20_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBK2388035.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(4006..5571)
                     /gene="ahpF"
                     /locus_tag="LGJ20_004030"
     CDS             complement(4006..5571)
                     /gene="ahpF"
                     /locus_tag="LGJ20_004030"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK2388036.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(5700..6263)
                     /gene="ahpC"
                     /locus_tag="LGJ20_004031"
     CDS             complement(5700..6263)
                     /gene="ahpC"
                     /locus_tag="LGJ20_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBK2388037.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            6635..7381
                     /gene="dsbG"
                     /locus_tag="LGJ20_004032"
     CDS             6635..7381
                     /gene="dsbG"
                     /locus_tag="LGJ20_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBK2388038.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            7590..8492
                     /locus_tag="LGJ20_004033"
     CDS             7590..8492
                     /locus_tag="LGJ20_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2388039.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            8639..9859
                     /locus_tag="LGJ20_004034"
     CDS             8639..9859
                     /locus_tag="LGJ20_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBK2388040.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            9832..10461
                     /locus_tag="LGJ20_004035"
     CDS             9832..10461
                     /locus_tag="LGJ20_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBK2388041.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(10462..11622)
                     /gene="ybdL"
                     /locus_tag="LGJ20_004036"
     CDS             complement(10462..11622)
                     /gene="ybdL"
                     /locus_tag="LGJ20_004036"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBK2388042.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            11731..12819
                     /gene="hcxA"
                     /locus_tag="LGJ20_004037"
     CDS             11731..12819
                     /gene="hcxA"
                     /locus_tag="LGJ20_004037"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBK2388043.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(12829..>12891)
                     /locus_tag="LGJ20_004038"
     CDS             complement(12829..>12891)
                     /locus_tag="LGJ20_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenoprotein"
                     /protein_id="HBK2388044.1"
                     /translation="EFFRERQDARYGGKGGARCC"
BASE COUNT         3322 a         3259 c         3064 g         3246 t
ORIGIN      
        1 ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg
       61 ccgtatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat
      121 tactggcgtc ttggcgcaat cttcggggtg atttacatct ctatgttgct gttggttggc
      181 tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc
      241 ccgccaggta agcccacctg gcgggctttt ttatgattta atagatagta ttcagtgtat
      301 gtatgagttc cacacccatt atgaaagcat tctggcgtaa tgccgcgttg ctcgcggttt
      361 ctctgcttcc cttctcttct gccaacgccg tagcgttgca ggcaaaacag tatggcgatt
      421 ttgatcgcta tgtcctggcc ctctcctggc aaaccggatt ttgccagagt caacacgatc
      481 gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac aaagctgatt
      541 ttctgaccgt acatggtctg tggccaggat tgcctaaatc ggttgctgcc cgtggtgttg
      601 atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat ctaccagaag
      661 cgcacgccag ccggatgtgt tcatcgccgg aaaccggatt atcactggaa acggccgcta
      721 aactaagtga agtcatgcca ggagctggcg gacgttcctg cctggaacgc tacgaatatg
      781 ccaaacacgg tgcctgcttt ggttttgatc cggacgcata cttcggtacg atggtacgcc
      841 tgaatcaaga aattaaagag agcgaagccg gaaaattcct tgcggataat tacggtaaaa
      901 cagtgagccg ccgtgacttt gacgccgcct ttgccaaaag ctggggaaaa gagaacgtga
      961 aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt cagatctcga
     1021 tcaaagctga cgccatcaac gctccgcttt ctgcaaactc attcttgcca caaccacacc
     1081 caggcaactg tggcaaaacc tttgtgattg ataaagtggg ttattaatca tctgatttac
     1141 aaaacacgct aattcgtctt ccataaaccg tgacgattaa gaattataca taatgcgctg
     1201 cattgaggaa ttccctgaat gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat
     1261 catcatgtga gttaaaccct cgccgcctga cggtgagggt tttcttttgg gtatgtttat
     1321 ctgcgacact cgcagtaccg acgacatgga gtaaaaatgt ccagaccaac tatcatcatt
     1381 aacgacctgg atgccgaacg catcgatatt ctgctggagc aacccgccta tgctggtttg
     1441 ccaatcgccg acgcgttaaa cgcagagttg gatcgcgccc aaatgtgttc gccagaagag
     1501 atgccacacg acgtggtgac aatgaacagc cgggttaaat tccgcaatct tagcgatggc
     1561 gaagtgcgtg tgcgcacgct ggtgtatccg gcaaaaatga ccgatagcaa tactcagctt
     1621 tccgttatgg ctccggtagg tgccgcactg ctggggctgc gcgttggcga ttccattcac
     1681 tgggaacttc cgggcggcgt tgcaacccac cttgaagtgc tggaactcga ataccagcca
     1741 gaagctgctg gcgactacct gctttaatcc catctgcaca agcgataact cgctttgccc
     1801 agaggatgca ctcgcatcct ctttacgcaa aatcagcaat ttttcctggt tctcactgtt
     1861 cacttttgct gcaaaattat tccgtttcgt ttgtgggaat aaccatggca gaagcatttt
     1921 acatcctgat aggatttctg attatggcgg cgattatcgt catggctgtc ctttacctcg
     1981 aaaaccactc ctgacgatca tattgttccc cccggcatcg cattcaccag acctgaaacc
     2041 gccttctgcg ccgcctctgc gctttgtgca ccgggtacca gaatcacctt acggcactcc
     2101 tcttcacgtt tttcgaaaat ctcatatccc cgggcggcct cttcaaacgg catatagtgg
     2161 gtaacaattt cttctggttt cagtaatcct ttctcaatta acggtaataa ttctcccagc
     2221 catgcgtgaa cgtgggtctg tcccatttta aacgtcaacc ctttatcaaa ggcgtcgcca
     2281 aacaggaaac cgtgaataaa tccagcgtag acgcccggta cgctaacaat gccgccacgc
     2341 ctgaccgccg caatacactg gcgcaacgct ttaccgctgc tgccctccag tttcaggtta
     2401 gtcagcaccg tttccgtggt gctgcctttc gcttcaaaac cgacggcgtc tattactgca
     2461 tccacgcccc ggtgacctgc cgtttgttca ataattgact gtgccggatc gctatcttca
     2521 tcgaaattaa tcgggatcgc gccgtagcgg tcagcggcga aacgcaagcg gtagggatga
     2581 tgatcaacaa caaaaatctg ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat
     2641 cccactggac cggcaccata gactgcaacg cttgaacctt gttggatctg cgcatttttt
     2701 gctgcctgcc atgccgttgg cagaatatca gaaaggaaaa gggctttatc atctgaaagc
     2761 aaaggcggta ctttaaacgg ccccacattc cctttaggga cgcggacata ttccgcctgc
     2821 ccaccaggaa cgccgccata caggtgacta taaccaaaca atgccgcggg agctggtatc
     2881 tgttttttat tgagcgcagc gcctttaccc gcattggtat tttcgcaggc ggcatattgc
     2941 tgcattcgac agaaaaaaca gtcgccacaa gcaatgacaa acggaattac cacccggtcg
     3001 cctttttgca aattttttac gtcctttccg gtttcaacga cttcccccat aaattcatga
     3061 ccaaaaatat cgccatgttt aacctggggt attttgcctc gataaagatg gaggtcagag
     3121 ccacagatcg ccgttgccgt aatacgcaga ataatatcat cagcctgttc aatgcccgga
     3181 tcgggaacat tttctacctg aacgtgatgt gggccgtgat aagtcaatgc tttcatgcaa
     3241 cctccagaga cagttcaaaa aagagtcagt ctttaagggt agctatcaca gcgggaaaac
     3301 gggcggtacc gcaggttttg ggagaattcc tggtgttcag tgagaatata ttaatcacag
     3361 aaaccgttcg taaggaaaaa gaaccagaca aatatcaggg ataaatatta taatcatggc
     3421 gaatgatttg ttcatgatta acagggagaa agggttatgt ataagacaat cattatgcca
     3481 gttgatgtat ttgaaatgga attgagcgac aaagctgttc gccacgctga attcctcgcc
     3541 caggatgacg gagttattca tctacttcac gtactacccg ggtcagccag cctgagcctg
     3601 caccgttttg ccgctgatgt gcgtcgtttt gaagagcatc tgcaacatga agcagaagaa
     3661 cgtctgcaaa cgatggtcag ccacttcacc atcgatcctt cccgcattaa acaacatgtc
     3721 cgttttggta gcgtgcggga tgaagtcaat gagttggcag aagaactggg ggctgatgtt
     3781 gtagttattg gttctcgcaa cccatcgatt tcgacccatc tgttaggttc taacgcctcg
     3841 agcgtgatcc gccacgccaa tctgccggtg ctggtcgttc gctaacagat aaaagtattt
     3901 atgccgccct gcctggaaca tcccggcagg gctttttaat gccataaaaa agccgccagg
     3961 tttgaccccg gcggctaagc aattgcaggt gaatcttact tcttcttatg cagttttggt
     4021 gcgaatcagg tagtcaaaag cactcagaga ggctttggca ccttcgccag tggcgatgat
     4081 gatctgcttg tacggaaccg tcgtacagtc acccgctgcg aacacgcctt tcacgttggt
     4141 ttcgcatttc gcatcaatga taatctcgcc catgcggtta cgttcgactg cgccttccag
     4201 ccagttggtg ttcggcagca gaccaatctg cacgaaaata ccggccagtt cgatgttgtg
     4261 aatatcgccg ctgacacgat ctcgatattc cagaccaacg actttgctgc cgtcgccttt
     4321 cacttccgtg gtttgcgcat tcagaataat gtcgacgttt ttcaggctgc gcagtttgtc
     4381 ctgcagaacc tggtcggctt tcatttctgg cgcaaattcc agcagagtta cgtgctcaac
     4441 gatacccgcc aggtcgattg ccgcttccac gccggagtta ccgccgccga taaccgctac
     4501 gcgtttgcct ttaaacagcg ggccgtcgca gtgcgggcag taggtcacgc ctttggtgcg
     4561 atactgatct tcgcccggaa cgttcatgtt gcgccatttt gcaccggtcg ccacgataat
     4621 gctgcgtgct ttcagtaccg cgccagaagc tgtttcaatc tggtgtagac caccttcaac
     4681 cgctgccggg atcagtttgc tggcgctctg gctgtcgatc acatcaacgt cgtattcatc
     4741 aacgtgaact ttcagtgcgc ctgccagctt ctgcccttca gtcttcggta cagaaatgta
     4801 gttttcgata tcaacggtat cgaggatctg accaccaaaa cgttcgccca tcagaccggt
     4861 acggatgcct ttacgtgcgg agtaaattgc tgccgctgca cccgccgggc cggaaccgac
     4921 gattaatacg tcataagcat cacgcttgtt cagctcttct gccgcacgtt tttccgcgcc
     4981 agtatcaatt ttggcaacga tttcagtcaa cgtcatgcgg ccctgaccaa actctttccc
     5041 gtttacgaac actgccggaa cgcccatcac gttgcgatcg gtgatttcgt tctggaaggt
     5101 gccgccgtca attgcagtgt gcttgatgcg cgggttcagt acgctcatca ggttcagcgc
     5161 ctgcaccacg tccgggcagt tgtggcaaga gagcgagtaa taggtttcga attcaaaatc
     5221 accgtcaata tggcgaatct gctccagcag agactgcgct tctttcgacg gatgaccacc
     5281 ggtccacagc aacgccagta ccagcgaggt gaactcgtgg cccagcgggg agcctgcaaa
     5341 acgtggcccc tggttggaac ctgggttggt gatcaggaaa gacggcttac gcaccggcaa
     5401 gctgttatct tctttaaagg tgactttgtc tgacagttct gcgatttcag ccaacagttc
     5461 cttgatttct gccgatttag cgctgtcatc cagcgtggca attaactcaa caggcttggt
     5521 caatttctca aggtaagcct tgagttgagt tttcatattt gtgtcgagca tgtttatctc
     5581 ctgggcttta aacatcatca tgcaagcggc ctctgcggct acctggatgc agcttgcatc
     5641 atggtgcggg gaagaaaatc gggtgccgca gcacccgatt aattacgcga ttgcggaact
     5701 tagattttac caaccaggtc cagagacgga gccagagttg cttcaccttc tttccattta
     5761 gccgggcaaa cttcacctgg gtgagaagct acgtactgtg ctgctttgat tttacgcagc
     5821 aggtcagacg cgtcacggcc aatgccttca gcggtaactt cgattgcctg gatgataccc
     5881 tgcgggtcaa caacgaaggt cgcacgatca gccagacctt catcttcacg catgttgtcg
     5941 aagttacggg tcagggcgcc agtcgggtcg ccgatcatcg catatttgat tttagcgatg
     6001 gtttcagagc tgctgtgcca tgctttgtgg gtgaagtgag tatcggtaga tactgcgtat
     6061 acgtctacgc ccagtttctg cagttcttcg tagtggtcag caacgtcacc cagttcggtc
     6121 gggcatacga aagtaaagtc agccgggtag aagaagaaga cgctccagcg gccttcggta
     6181 tctttttcgg tgatttcgat gaattcgccg tttttgaatg cctggttttt aaaaggttta
     6241 attttggtgt taatcaagga catctatact tcctccgtgt tttcgatgag atgtaaggta
     6301 accaattttt gccgattcgg ctaatgcgtt tccattatca aatcgataag ttttacctta
     6361 caaccttcgt aagacaactt tgtgaacttt gcaccgccag cggcgataaa aagtaaaatg
     6421 gccccctttc caggtgaccg tgtaatctga actacccttt ggtatcttca gcgcctgcct
     6481 gcaggctgag gtgttgccct tacaaatgca acaacgacat ggattacaac accctcataa
     6541 acaaagggca atcacctgat ctaagctctt acctatgaca gtgataggtt atgcctttta
     6601 ctcgactttt gcactgactg aaaaggacaa attaatgtta aaaaagatac ttttactggc
     6661 tctgcttcct gcaatcgcct tcgcagagga acttccttcc ccagtaaaag cgattgaaaa
     6721 acagggcatt acaatcatca aaacgttcga tgcccccgga ggaatgaaag gttatctcgg
     6781 aaagtatcag gatatgggcg tcaccatcta cctgactccc gatggtaagc acgctatctc
     6841 tggttacatg tacaacgaga aaggtgaaaa cctgagtaac acacttatcg aaaaagaaat
     6901 ttacgcacca gccggacgcg aaatgtggca acggatggaa caatcccact ggctcctcga
     6961 cggtaaaaaa gatgcgccgg tcattgtcta cgtgttcgcc gatccgttct gcccatattg
     7021 taaacagttc tggcagcagg cgcgcccgtg ggtagattct ggcaaagtgc aattaagaac
     7081 attgttggtt ggggttatca agccagaaag cccggcgaca gcagcggcaa ttcttgcctc
     7141 caaagatccc gcaaaaacct ggcaagaata tgaatcctct ggtggcaagc ttaagctaaa
     7201 cgtgcctgca aacgtaagta cagaacaaat gaaagtgtta agcgccaacg agaaactcat
     7261 ggacgatctg ggggcaaatg tcacaccagc gatctattac atgagtaagg aaaatacgct
     7321 gcaacaggcc gtggggttgc ccgatcagaa aacgcttaat atcattatgg ggaataaata
     7381 aaagataatt catggatggt tgaaatacat ccctgctttt caaccatcct aaaaaagcat
     7441 gaattgctcg ctctgcccta agtggctatg ctattttaga aaaacttaat cctgagttaa
     7501 gcaaaaaaat ataatcaata aaaatatata cgcaaaaaac attgattaag tgaatatacc
     7561 atggaagaaa aatataaccg gagtagtgta tggccaatct ctacgacttg aaaaagttcg
     7621 acttaaacct tcttgtcata tttgagtgta tttaccagca tttaagcatt agcaaagctg
     7681 ctgaatcact atatatcacc ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc
     7741 aatttaatga ccctttattc attcgctcag gaaaaggtat cgcgccgacg acaacagggt
     7801 tgaatctgca tcaccatctt gagaaaaacc tcaaaggcct tgaacaaaca attaatatag
     7861 tcaataagtc cgaactaaag aaaaacttta ttatttacgg ccctcagctc atttcttgct
     7921 ctaataatag tatgctgatc cgctgtctac ggcaagatgc ctctgtcgag attgagtgcc
     7981 atgatatcct catgtcagca gagaatgctg aagaactgtt ggttcaccgc aaagccgatc
     8041 tggtgatcac ccaaatgcca gtgatcagcc gttccgtcat ttgcatgcca ttgcatacaa
     8101 ttcgcaatac tctaatttgt agtaataagc atccgcgaat tactgacaat agcacttatg
     8161 aacaaattat ggcagaagag ttcacccaat taatatccaa atcagcagga gttgatgata
     8221 ttcaaatgga aatagacgag aagtttatga atcgcaaaat ttcatttcgt ggttcctctt
     8281 tgctgacaat cattaatagc attgcagtta cagatttact gggaatagtc ccctatgaac
     8341 tttataattc ctaccgtgac tttcttaatt taaaagagat aaaactagaa catccgttac
     8401 cttcaataaa gctttacatt tcctataata aatcttcttt aaataatttg gtgttctccc
     8461 gatttattga ccgtctaaat gagagttttt gatatataac tacagacagc tactataact
     8521 ttcatctatt tattcacagc gcgaatgaaa aataaaacac tctgctttgc agtgtttact
     8581 ttataattaa cagacttatc tcactgatca ccctgtaacg ttcagagagc gtcttcagat
     8641 gtctatttat aaaattcctc ttccgctcaa tattctggaa gctgcacgag agcgtattac
     8701 ctggacactc aacaccctac cccgcgtttg tgtctcattt tctggcggca aagactctgg
     8761 tttaatgctg catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt
     8821 gtttatcgac tgggaggcgc aattctcttg caccattaac tatgttcagt ccctgcgcga
     8881 gttgtacacc gatgtcatcg aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa
     8941 ttccctttca caattccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg
     9001 tcagcccccg caagatgcga taaccgatcc tgactttttc tgcttttacc agcctggcat
     9061 gaccttcgaa caatttgtac gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc
     9121 gatgatgatc ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt
     9181 aaataaacaa cgttttgccg acgataaacc atggactacg gccgcaccag gcggtcatag
     9241 ctggtacatt taccccattt acgactggaa agtggctgat atctggacct ggtatgcaaa
     9301 tcatcaaagt ctctgcaacc cactgtataa cctaatgtat caggcaggcg ttcctctgcg
     9361 tcatatgcga atttgcgaac cttttggccc ggagcaacga caaggattat ggctctatca
     9421 cgttatcgaa ccggatcgct gggcagctat gtgcgcacga gtcagcgggg taaaaagtgg
     9481 cggcatttac gccggacatg acaatcattt ctatgggcat cggaaaatcc tcaagccaga
     9541 acatttagac tggcaagaat atgcattatt gctgctcaat agcatgccgg aaaaaacagc
     9601 tgagcattac cgcaataaaa tcgccattta tttgcactgg tatcagaaaa aaggcatcga
     9661 ggttccacaa acccagcaag gggacattgg cgcgaaagat atcccctcct ggcggcggat
     9721 atgcaaagtt ttactcaata acgattactg gtgtcgagca ttatcattta gtcctacgaa
     9781 agcgaagaac tatcagcgtt ataacgaacg gataaaagga aaacgtcagg aatgggggat
     9841 actatgcaac aacgattaac gcaggattta acgcaatttc tcgccagctt gccggaagat
     9901 gatcgcatca aagcgattaa tgaaatccgc atggcgatcc atcaggttag cccctttcgt
     9961 gaagagccag tggattgcgt tctgtgggtt aaaaacagcc aacttatgcc taatgattac
    10021 aacccaaaca acgtggcacc acccgaaaaa aaactgctga aaaaatccat tgaaatagat
    10081 ggttttaccc aacccattgt ggtcacgcat acggataaaa atgccctgga aatcgtggat
    10141 ggttttcacc gccatgaaat tggtaaaggc tcatcatcat taaaattacg cttaaaaggt
    10201 tatttgccgg tgacctgtct ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt
    10261 cataatcgcg ctcgtggacg ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt
    10321 agccagttgg gatgggacga taataaaatt ggcaaagagc tgggaatgga cagcgacgaa
    10381 gtgctgcgcc tgaagcaaat taacggcctg caagagctgt ttgccgaccg tcaatattcc
    10441 cgcgcctgga cggttaaata actaaagctg gcgcaggcgt tcagctgctg ccagcaacgt
    10501 cgattccttc ttggcaaaac agagacgaat cagtttatgt gggaagggat cggcgcaaaa
    10561 caccgacagc ggaatcgccg ctacgccgtg ctcctgcgtc agccactggc aaaactcaac
    10621 atcatccagg gtagaaacct cgctgtaatc caccagcaaa aagtatgtac cttcacacgg
    10681 taaaatctcc agccggcttt catttaaagc attcaccaga atatcgcgct tctggcgata
    10741 aaagtccggt aacgcaagat aatgctcagg ttctgcacgt agcatatcag caagcgccag
    10801 ctgtgccggg gtattcaccg aaaaggtcag atactgatgt accttgcgaa tttcggcgct
    10861 gatgggcgct ggcgcaacac aataacccac tttccagccg gtcatatgat aggtcttgcc
    10921 aaatgaagaa accgccactg cccgctcacg cagctgcgga tgcgccagca cactggcatg
    10981 gccctgttgt gaaaagttga tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg
    11041 cccggcgatc gcctgccaca aagcggcgaa atcagcctgc tgccagatag ttgcactggg
    11101 gttatgcgga gtgttgagga tcaccagtct ggtgcgctcg cttaataatg cggcaaattc
    11161 ctgccagtca acgcgaaaat gcggtggttg cagtgccata cgcttcacta ttcccccaga
    11221 aagcgcgatg gcgggggagt aactgtcata gctgggatca aaacaaatca cttcatcgcc
    11281 attgcgcacc agtgcggtaa tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt
    11341 gatatcgcta tcggcatctg gttgatagcc atacaaacgt tccgttttct gagcaatcgc
    11401 ctcgcgcaag gcctgcacgc cggtcatggg cgcgtattgg tttgccccct gggcaacgtg
    11461 gtacgccagc cgctcctgta aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag
    11521 gttaatcgcc tggtgttgct gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag
    11581 ttgtggaagt ttgctttgtg gaatcagagg gttatttgtc atttttattg tacctgacga
    11641 agttgcgtgt tgaaggcact ataacacgat gctagtattt ggcaatcaag acgtttagat
    11701 gtctaaataa aacaataagg acaacacaac atgcctcaca atcctatccg cgtggtcgtc
    11761 ggcccggcta actacttttc acatccagga agtttcaatc acctgaacga ttttttcact
    11821 gatgaacaac tttctcgcgc ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa
    11881 accaaacttc cgccagcgtt tggacttcca ggggcaaagc atattttgtt tcgtggtcat
    11941 tgcagcgaaa gcgatgtaca gcaactggcg gctgagtccg gtgacgatcg cagcgtggtg
    12001 attggcgtcg gcggcggcgc actgctcgac accgcgaaag ccctcgcccg ccgtctcggt
    12061 ctgccatttg ttgccgctcc gacgatcgcc gccacctgcg cagcctggac accgctctcc
    12121 gtctggtata acgatgccgg acaggcgctg cattatgaga ttttcgacga cgccaatttt
    12181 atggtgctgg tggaaccgga gattatcctc aatgcaccgc aacaatatct gctggcgggg
    12241 atcggtgaca cgctggcgaa atggtatgaa gcggtggtgc tggctccgca accagaaacg
    12301 ttgccgctaa ccgtgcggct ggggatcaat aatgcgcagg ccattcgcga cgtcttgtta
    12361 aacagtagcg aacaggcact tgccgatcag caaaatcagc agttaacgca atcattttgc
    12421 gatgtggtgg atgcgattat cgccgggggt ggaatggtcg gtggtctggg cgatcgtttt
    12481 acgcgtgtgg ctgcggccca tgccgtgcat aacggtctga ctgtcctgcc gcaaaccgag
    12541 aaatttctcc acggcaccaa agtcgcttac ggaattctgg tgcaaagcgc cttgctgggt
    12601 caggatgatg tgctggcgca attaaccggg gcgtatcagc gttttcatct gccgactacg
    12661 ctggcggagc tggaagtgga tatcaataat caggcggaga tcgacaaagt gattgcccac
    12721 accttgcgcc cggtggagtc cattcattac ctgccagtca cgctgacacc agatacgttg
    12781 cgtgcagcgt tcgaaaaagt ggaatcgttt aaagcctgac gtgcaaattt agcagcagcg
    12841 cgcgccgcct tttccaccgt agcgcgcgtc ctgccgctca cggaaaaact c
//
LOCUS       DAFATA010000117        12652 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFATA010000117 DAFATA010000000
VERSION     DAFATA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.118"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..269)
                     /locus_tag="LGJ20_004039"
     CDS             complement(<1..269)
                     /locus_tag="LGJ20_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK2388045.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLG"
     gene            complement(262..1461)
                     /gene="lolC"
                     /locus_tag="LGJ20_004040"
     CDS             complement(262..1461)
                     /gene="lolC"
                     /locus_tag="LGJ20_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBK2388046.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            1723..2796
                     /locus_tag="LGJ20_004041"
     CDS             1723..2796
                     /locus_tag="LGJ20_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBK2388047.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            2924..6370
                     /gene="mfd"
                     /locus_tag="LGJ20_004042"
     CDS             2924..6370
                     /gene="mfd"
                     /locus_tag="LGJ20_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK2388048.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKSGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            6514..7476
                     /gene="ldtC"
                     /locus_tag="LGJ20_004043"
     CDS             6514..7476
                     /gene="ldtC"
                     /locus_tag="LGJ20_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBK2388049.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(7559..7816)
                     /gene="bhsA"
                     /locus_tag="LGJ20_004044"
     CDS             complement(7559..7816)
                     /gene="bhsA"
                     /locus_tag="LGJ20_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBK2388050.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            8057..8689
                     /gene="comR"
                     /locus_tag="LGJ20_004045"
     CDS             8057..8689
                     /gene="comR"
                     /locus_tag="LGJ20_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBK2388051.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(8751..9290)
                     /locus_tag="LGJ20_004046"
     CDS             complement(8751..9290)
                     /locus_tag="LGJ20_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBK2388052.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(9517..10821)
                     /gene="ndh"
                     /locus_tag="LGJ20_004047"
     CDS             complement(9517..10821)
                     /gene="ndh"
                     /locus_tag="LGJ20_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBK2388053.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(11229..11771)
                     /locus_tag="LGJ20_004048"
     CDS             complement(11229..11771)
                     /locus_tag="LGJ20_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2388054.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(11794..>12652)
                     /gene="nagZ"
                     /locus_tag="LGJ20_004049"
     CDS             complement(11794..>12652)
                     /gene="nagZ"
                     /locus_tag="LGJ20_004049"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBK2388055.1"
                     /translation="LVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEA
                     GWLMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGM
                     KTTGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIY
                     SDVDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCD
                     MILVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHE
                     RWQEEKAGH"
BASE COUNT         3033 a         3309 c         3212 g         3098 t
ORIGIN      
        1 cccagcttct ggttgcgcag ctctgctttc gccgccgaag acagtttgct catcggttga
       61 ccgttaaaga tcacatcgcc ggaggttggc gtatccagcc cgcccaacaa gtgcagcaag
      121 gtgcttttac cggaaccaga gctaccgacg atcgccatca tttcaccttc gccgacgctg
      181 aaactgacat tgtgcagcac atcggtttgc acactgcctt cctgatagcg tttgcacagg
      241 ttgtcgcatt gcaacaggat cttattcata acgtaaagcc tcagcgggtt gagtggcggc
      301 agcgcgccat gaagggtaaa gcgtagacag cagcgcgata gccatcgcca ccagcgcaat
      361 aacaatgacc tgtaaaggtt cgatagcgac cggcagcgcc gcgccatcaa gcaggacgcc
      421 gattatcggc atcagattat ttaactggct ggcaagcagg gcgccaagcg ccgctccgag
      481 gatcgcaccg ataatcccgg cgctggcccc ttgcaccata aagaccatca tgatttgtcg
      541 cggagttaag ccttgcgttt gcaggatcgc tacttcgccc tgcttctcca ttaccatcag
      601 ccctagtgag gtaataatgt taaacgccgc aacggcgaca atcaggctca gcagtaaacc
      661 catcatattt ttttccatgc gtacggcctg gaacaactcg cctttacgat cacgccagtc
      721 ctgccatttg ctgccttcag gcagtttttg ctgacttaat gagtcgactt tcagcggctc
      781 atccagccac aaacgccagc cggtaatatt gcctgccgga taacgcatca gacgcgaggc
      841 atcctcaata ttcaccagca tttcatagcc atcgacttca ctgttggcgg cgaaagtgcc
      901 aatcacattg aacaggcgct ggcttggaat acgccccatc ggcgtgaact ggctggcaga
      961 tggtaccatc acgcggattt gatcaccgcg attaacgcct agctgtgagg caagttgttc
     1021 gccgaggatg acattatatt tccccggctc gaggtcagtt tgtttcacat tgaccagata
     1081 cggtgtaagt ggatcttttt gcgccgggtc gataccgagc atcaccccga ccgccacgct
     1141 gcgcgcgctt tgcagtacca catcaccggt agtaataggt gcgacgcgat taacgccgtc
     1201 cagtttgact gccgtttccg ggagttgctg cgggttaaga gagccatgct cagaagagag
     1261 aattgcctgt ggcatcaggc caaggatgtt gttttgcagc tcgcgctcaa agccgttcat
     1321 cactgacaat actgtgacca gcgccatcac cccgagggta atgccgatgg tagaaagcca
     1381 ggagacgaaa cgaccgaagc gatccgctgc acgcccacgc atgtaacgca ggccaataaa
     1441 tagagcgaca ggttggtaca tgaaatccgt ctggttgctg tagcaaagac acgagtatat
     1501 aagcgaaagc aagatgggta aacgtctgaa ctgtaagtga tcgtcagggc taatctgaaa
     1561 agcccccgtg aatcaatcgg ctaaattgcc atgttttatc atcaatcttg ccgcctgatc
     1621 ttaatctgaa aaatcatcca atacagacag atacccatta cacatagggt cataacccgc
     1681 aggatcaacc gcatgtaaat taaaaaagag cgtgttgtcc tgatgaaaca aaaagagcta
     1741 tggattaacc agatcaaagg gttatgtatt tgtctggtgg tgatttatca ctcggtcatt
     1801 accttttatc cgcatctgac cacttttcag catccgttat cggaagtcct gagcaaatgc
     1861 tggatctatt tcaatcttta ccttgcaccc tttcgtatgc cggttttttt ctttatttcc
     1921 ggctatttga ttcgccgcta tatcgacagc gtaccgtggg gaaattgtct cgataaacgc
     1981 atctggagca tcttttgggt gctggcgctt tggggcgtgg tgcagtggct ggcgttaagt
     2041 gcgcttaacc agtggctggc acctgagcgc gatttaagca acgcttccaa tgccgcgtat
     2101 gccgattcca ccggtgagtt cctgcacggg atgatcacag ccagcaccag cttgtggtat
     2161 ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt ttagccgtct ggcgctgcca
     2221 ctattcgcct tgtttgtact gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg
     2281 ggaatgaaca gtgtgatccg caatttgcct tattacagcc ttggcgcatg gtttggcgca
     2341 acattaatga cctgtgttaa agaggtgccg ttgcgccgcc atctgctgat ggcttctttg
     2401 cttaccgttc tggcggtcgg tgcctggttg tttactatct cgctgctgtt gtcgctggta
     2461 tcgattgtgg tgatcatgaa gctgttttat caatacgagc aacaattcgg tatgcgctcc
     2521 accagcctgc tgaatgtgat tggctccaac accattgcta tctacaccac ccatcgcatt
     2581 ctggttgaaa tattcagctt aactctgctt gcgcaaatga acgcagcacg ctggtcgccg
     2641 caagtcgaac tgacactcct gctggtttat ccctttgtca gtttgttcat ctgtactgtt
     2701 gcgggcttgc tggtaagaaa actttcacag cgtgcattca gcgatctgtt gttctccccg
     2761 ccttcactgc ccgctgccgt cagttactcc cgctaagcca tcgccccagc cggggcgatt
     2821 caatttgcta aaccatgtcg attacggata atgaacagca ttgcttatca gagatatgcc
     2881 cccatatgtt gaggcatatc ctaacgagaa tctgacaacc gttatgcctg aacaatatcg
     2941 ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg ggcgagttaa ccggcgcagc
     3001 ctgtgcaacg ctggtagcgg aaattgccga acgtcacgcc ggtccggtgg tactcattgc
     3061 accagatatg caaaatgctc tgcgtttgca tgatgaaatc agccagttca ccgatcagat
     3121 ggtgatgaat ctggcggact gggaaactct tccctacgac agtttttcgc ctcatcagga
     3181 cattatctcc tcgcgccttt ccacccttta ccagctaccg acgatgcagc gtggcgtact
     3241 gattgttccg gtgaatacgc ttatgcagcg cgtttgccca cacagttttc tccacggtca
     3301 tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat gcattacgaa cccaactgga
     3361 cagcgccggt tatcgccatg ttgaccaggt gatggagcac ggcgaatacg ccacgcgcgg
     3421 cgcgttgctg gatctcttcc cgatggggag tgagctgcct tatcgtcttg atttctttga
     3481 tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt
     3541 agaagcgatc aatctgctgc ccgcgcacga atttccgacc gataaagcgg caattgaact
     3601 gttccgcagc cagtggcgcg ataccttcga agtgaagcgc gatccggaac atatttacca
     3661 gcaagtgagt aaaggcacat tacctgccgg gatcgagtac tggcagccat tgttcttcag
     3721 cgaaccactg ccgccgctgt tcagttattt ccctgccaat accttgctcg tgaatactgg
     3781 cgatctggaa accagtgccg aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg
     3841 cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg ctctggctgc gggtggacga
     3901 gctcttctca gagctgaaaa actggccccg ggtgcagcta aaaactgaac atttaccgac
     3961 aaaagccgcg aatgccaatt taggtttcca gaaactgcca gacctggccg ttcaggcgca
     4021 acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag actttcgacg gtccggtggt
     4081 gttctcggta gaaagtgaag gtcgccgtga agcgctgggt gaactgctcg cacgaattaa
     4141 aattgctccg caacgcatta tgcgtcttga tgaagccagc gaccgtgggc gttatctgat
     4201 gattggcgct gccgaacatg gttttgtcga taaggtgcgt aatctggcgc tgatctgcga
     4261 aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag gattctcgcc gcaccatcaa
     4321 ccccgataca ctgatccgta accttgcgga gctgcatatt ggtcagccgg tggtccatct
     4381 ggagcacggt gtcgggcgct atgccggaat gaccacgctc gaagcgggcg gcattactgg
     4441 cgagtatttg atgctcacct atgccaacga cgccaaactg tatgttccgg tgtcgtcact
     4501 gcatctgatt agccgttacg caggtggcgc ggaagaaaac gccccgctgc ataaacttgg
     4561 cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc
     4621 ggaattgctg gatatctacg cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga
     4681 tcgtgagcag tatcagttgt tctgcgacag cttcccgttt gaaaccacgc cggatcaggc
     4741 gcaggccatt aatgcggtac ttagcgacat gtgtcagccg ctggcaatgg atcgtctggt
     4801 gtgcggcgat gttggctttg gtaaaacaga agtggcgatg cgcgccgctt tcctggcagt
     4861 agataaccac aagcaggtgg cggtgttggt gcctaccacc cttctcgcgc agcagcatta
     4921 cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt atcgaaatga tctcccgttt
     4981 ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat
     5041 tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag tttaaagatt taggcctgct
     5101 gattgtcgat gaagaacacc gcttcggggt gcgtcataaa gagcgtatta aagcgatgcg
     5161 cgcgaacgtg gatattctga cgcttactgc aacgccgatc ccacgtacgc tgaatatggc
     5221 aatgagcgga atgcgtgacc tgtcgattat cgccacgccg cccgcccgtc gtctggcagt
     5281 taaaaccttt gtccgtgagt atgacagcct ggtggtccgg gaggcgatcc tgcgtgaaat
     5341 tttgcgcgga ggacaggttt attatctcta caatgatgtg gaaaacattc agaaagccgc
     5401 cgaacggctg gcagaactgg tgccagaagc gcggatcgcc atcggtcacg ggcagatgcg
     5461 cgagcgcgaa ctggaacggg tgatgaatga tttccatcat caacgtttca acgtgctggt
     5521 ttgtaccacc attatcgaaa ccgggatcga catcccgaca gccaacacca ttatcattga
     5581 acgtgcggat cacttcggtc tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc
     5641 gcatcatcag gcatatgcat ggttgctgac accgcatcca aaagcgatga ctaccgatgc
     5701 acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc
     5761 aacgcacgat ctggagattc gcggcgcggg tgaactgctt ggcgaagaac aaagtggctc
     5821 aatggaaacc atcggtttct cgctgtatat ggagttgctg gaaaacgccg tcgatgcact
     5881 gaaatccgga cgcgagccgt cgctggaaga tctcaccagc cagcaaacag aagtcgagct
     5941 gcggatgccg tcgctattgc cagatgattt cattcctgac gtgaatacgc gtttgtcgtt
     6001 ctataaacgt attgccagcg ccaaaacgga aaacgaactg gaagagatca aagtcgagct
     6061 tatcgatcgc ttcggcctgc tgccggatcc ggcgcgtacc ctgctggatg ttgcccgtct
     6121 gcgccagcaa gcgcagaaac tggggatcag gaagctggaa ggtaatgaga aaggcggggt
     6181 gatcgaattt gccgagaaga atcacgttaa tccggcctgg ttgattggtt tgctgcaaaa
     6241 acagccgcag cattaccgcc ttgatggtcc gacgcgcctg aaatttattc aggatttgag
     6301 tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg cgtgaactgg aagagaacgc
     6361 gatcgcttaa tggtaaatcg ggggcgtttc tgcgccccca tctgcaacat ttacaaattc
     6421 tttgcacttc cctgcactat ccgacactgt caccatccat aattcaggct tatctgttta
     6481 ttacaataac cttatattta ttatggattt ttggtgatga tcaaaacgcg tttttctcgc
     6541 tggctaacgt tttttacgtt cgccgctgcc gtggcgctgg cgctaccggc aaaagccaac
     6601 acctggccgc tgccgccagc gggcagtcgt ctggttggcg aaaacaaatt tcatgtggtg
     6661 gaaaatgacg gtggttctct ggaagccatc gccaaaaaat ataacgtcgg ctttctcgct
     6721 ctgttacagg ctaaccctgg cgttgatcct tacgtaccgc gcgcgggtag cgtgttaacg
     6781 atcccgttgc aaaccctact tccagatgcg ccgcgcgaag gcattgtgat caatattgcg
     6841 gagctgcgtc tctattacta cccgccgggt aaaaattcgg taaccgtgta tccaataggt
     6901 attggtcagt taggtggtga cacgctgaca ccgacaatgg tgaccaccgt ttcagacaaa
     6961 cgtgcaaacc caacctggac gccaacggca aacatccgcg cccgttataa agcacaggga
     7021 attgagttgc ctgcggtagt gccggctgga ccggataacc caatgggcca tcatgcgatt
     7081 cgtctggcgg cctatggcgg cgtttatttg cttcacggta cgaacgccga tttcggcatt
     7141 ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg acgatatcaa aacactcttt
     7201 agccaggtca ccccaggcac caaagtgaat atcatcaaca ctccgataaa agtctctgcc
     7261 gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc tgtcagagaa gattgatgac
     7321 gatccgcagc tgctgccaat tacgctgaat agcgcaatgc aatcatttaa agatgcagca
     7381 caaactgacg ctgaagtgat gcaacatgtg atggatgtcc gttccgggat gccggtggat
     7441 gtccgccgtc atcaagtgag cccacaaact ctgtaaatcc tggataaaaa aaatccccgc
     7501 gacacggcat gtcgcgggga aaaaatcagc agtagcaggc attaatgagg gttaatgctt
     7561 atttataaat tactgctgtt ccatggaggg tattcggacc ggttacagaa gtaatacgga
     7621 aagattttgc gcccatctca tccgcttttt gcgccagctg ctcttccagc gatcccagat
     7681 ttgtccccgc gttagcactg attgtaccga ctttttgttg gccttctggc gttgactgaa
     7741 cttcgacagc cgcaaagctg gcaaatgaca tggagcttaa aatcgccgca gcgatgaggg
     7801 tttttacgtt tttcataata gtggccttat gcagatgaat gacgtcgcaa gcaattactt
     7861 aacgatcgat aactaaatga tagatgtgat ctggatcaca tacaagatat tttttataac
     7921 aatcattaat taatttaaaa atctttaaat ttcagttaaa tagaaagcaa ttatttttat
     7981 gggatctgga ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac
     8041 caggtacaac ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg
     8101 cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcaaca
     8161 cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg accggagcca aagcgcccac
     8221 gctatacgcg gaatttacca acaaagaggg gttatttcgt gccgttctcg accgctatat
     8281 cgatcgtttt gccgctaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc
     8341 tgcgctggct gactattttg ctgccatcgc caactgcttt accagcaaag acaccccggc
     8401 aggctgcttc atgatcaaca actgcaccac cctctcccca gattcaggag atatcgccaa
     8461 tacgttgaaa tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg
     8521 acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca catctggcag aattccttaa
     8581 ttgtattatt caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg aaaaactaat
     8641 gcagattgcc ggaacgactt tgcgtttatg gcccgaactg gtgaaataag tcgcaaaaaa
     8701 agcccctcag cctgagcgaa tgaggggcca aattgcagag tacagccttg ttatactttg
     8761 ttattcaaaa tcagttgccc attactatct agcgggatct gcgtacccgg atcgcggtcc
     8821 atgcggattt tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt
     8881 tctgacttgt catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc
     8941 tggagagagc cctggatctg gttaccggca tatccaccac ccagcgcccc cacaacagtg
     9001 gcgacatctt taccacgacc accaccaaac tgatgcccta tcacgccgcc agcaacagcg
     9061 ccgagcaccg acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg
     9121 acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca
     9181 acctgagcgt attgcgggcc ccgttcaaac acgttcagac tggccactgc cgctacgccc
     9241 agcgcagctg cgacaccaat cccgataccc gccaacattg atttattcac gggaacacct
     9301 ccttctttgc agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag
     9361 atgaaaagcg cgggaatggc tggaaatact taaggattcg gaggagtctg aaggaatgcc
     9421 tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga
     9481 tgaggcgttt atgccacatc cgccagtgta cgtcgattaa tgcaacttca aacgcggacg
     9541 gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag
     9601 cgcaatctga tgcattcggt atagcgagat atatacaaag cgcgcaattc gtccttcaat
     9661 catcattgag ccgcgcgtca ggttacccat caggctaccg acggtggaga agttcgacag
     9721 cgataccagc gaaccatgat ctttatactg ataatttttc agcggcttac cgttcatctg
     9781 cgccagtatg ttgttcattg cgcaagtcgc catctggtgt gcagcctgag cacgcggcgg
     9841 aacaaagccc ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt aaatgtctgg
     9901 atcgcgggtg gtttgcagcg tcggttccac caccagctgg ttgatacggt tagtttcaag
     9961 accaccgata tctttcagga agtctggcgc tttgatcccg gctgcccaca ccatcagatc
    10021 agcctcaata tattcgccat ctttagtgtg caggccgcct tcatcagcac tggtgaccat
    10081 ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg cagcagagat
    10141 acgtggcggt aatgcaggca aaatacgttc tcccgcttct accagcgtta cgttcagggc
    10201 ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc ttgaccgcgt tgtgcaattc
    10261 agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttca ctttgccatt
    10321 tgcgcccagg ttggcggagt atttcaggaa gagattcagc atctcctggt gaaagcgacg
    10381 cgcctggtgc gggttatcga ggaaaatgca gttctctttg acacctggcg tattgaaatc
    10441 gttagaggtg ctacccagcg ccattaccag ggtgtcatag gcgattttac gttccggaac
    10501 cagcagttca cctttctcat cgcgcagttc tgcaatagtg attgttttcg cttcacgatc
    10561 aatatcaatg acggaaccca gctggaactg gaaaccatga ttgcgcgcat gggccagata
    10621 gctcaacgca tcgacgcctt catcaagcga gccagtcgcc acttcgtgca gcagcggttt
    10681 ccacaggtgg ctgtggttac gatcgaccag cgtaattttg gcttttttct tacgtcccag
    10741 cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat
    10801 ctttttcaat ggcgtagtca acgtgacccc cttaaattta ttaaccaatt gttaattaat
    10861 ggttattaac atagctctta attaacaaca ggttacagca atgaaaatgt tcttcgctat
    10921 tgagaataac atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg
    10981 ctgaaaagat aggcgacttt accgggtgag atcaaaaaat agagtttatt gtttattatt
    11041 tgagcaaatt cggaagagta atatagggtg aagcgtatat cgagggcgtt tggtcgtcct
    11101 gataagatgc gcagcatcgc atcaggcatt gatgctcgac tgtcggatgc ggcgtggacg
    11161 ccttatccga cttacagaat tcggagcgca tcaggtaccg gtaaggttac ctgatgcgac
    11221 gagggcattt acccgagggt tttgaacgct ttaatgcgct gtaaatgcgg ggagatattc
    11281 ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg caactcttca
    11341 gatgtccgct ggctgttaag tgcttcatca ttacgcgaca aaataaccag acaacgatcg
    11401 cgattcttct cgcggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg
    11461 cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg
    11521 cggatatcgc aaagaaaacc aatccgttcc gcccagtatc cgcccaggcc aacgccgcaa
    11581 attagcggac gctcgtcaac gttcagttgc aacattttgt ccacttcttt aagcagatgc
    11641 tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata
    11701 aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa
    11761 tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca
    11821 gcgttcgtgt aactgattca gacgggtgct gatcgctttc cagcgagccg agtccatcag
    11881 ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg
    11941 tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca ggatcatatc
    12001 gcaacccgca tccagtgatg cctgcccgcg ttcggcataa ctgcccataa tcgcggcacc
    12061 ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa cccagttcct gacgcaaaac
    12121 ggttttcagc cagtagggag aaccgctcgc cggacgcgga tcaacatcac tgtagatcac
    12181 atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt aaggaactga agaccgacat
    12241 atctttagcg cgaatctccg cttgtggacg tggatcgcac ggtgtttctt tgtgtgagtc
    12301 tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc gttttcattc cggcttcatg
    12361 cataccatca ataaaccggc tggcaattgc cagggctttt tgtggatcgg catgataaga
    12421 acgctcgcca atcgccgcgc tgatatgccc gacatccagc actggcgcaa agctgatatc
    12481 aatatccata gcgatcattt cgctggccat cagccaaccc gcctcttgcg ccagtttgcc
    12541 gccctcttcc attcctgaca gcgcagcgaa tgattgcgcc gctggcaagc gggtaaaacc
    12601 ttcacgaaaa cgctgcacgc gtccaccttc ctgatctacc gccaccacca ga
//
LOCUS       DAFATA010000118        12618 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFATA010000118 DAFATA010000000
VERSION     DAFATA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.119"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..176)
                     /gene="galM"
                     /locus_tag="LGJ20_004050"
     CDS             complement(<1..176)
                     /gene="galM"
                     /locus_tag="LGJ20_004050"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="mutarotase; catalyzes the conversion of
                     beta-galactose to the alpha-anomer; links the metabolism
                     of lactose and galactose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBK2388056.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASP"
     gene            complement(170..1318)
                     /gene="galK"
                     /locus_tag="LGJ20_004051"
     CDS             complement(170..1318)
                     /gene="galK"
                     /locus_tag="LGJ20_004051"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK2388057.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(1322..2368)
                     /gene="galT"
                     /locus_tag="LGJ20_004052"
     CDS             complement(1322..2368)
                     /gene="galT"
                     /locus_tag="LGJ20_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBK2388058.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFREFGV"
     gene            complement(2378..3394)
                     /gene="galE"
                     /locus_tag="LGJ20_004053"
     CDS             complement(2378..3394)
                     /gene="galE"
                     /locus_tag="LGJ20_004053"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK2388059.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(3655..5127)
                     /gene="modF"
                     /locus_tag="LGJ20_004054"
     CDS             complement(3655..5127)
                     /gene="modF"
                     /locus_tag="LGJ20_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBK2388060.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(5195..5983)
                     /gene="modE"
                     /locus_tag="LGJ20_004055"
     CDS             complement(5195..5983)
                     /gene="modE"
                     /locus_tag="LGJ20_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBK2388061.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6112..6261
                     /gene="acrZ"
                     /locus_tag="LGJ20_004056"
     CDS             6112..6261
                     /gene="acrZ"
                     /locus_tag="LGJ20_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBK2388062.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6428..7201
                     /gene="modA"
                     /locus_tag="LGJ20_004057"
     CDS             6428..7201
                     /gene="modA"
                     /locus_tag="LGJ20_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2388063.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            7201..7890
                     /gene="modB"
                     /locus_tag="LGJ20_004058"
     CDS             7201..7890
                     /gene="modB"
                     /locus_tag="LGJ20_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBK2388064.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            7893..8951
                     /gene="modC"
                     /locus_tag="LGJ20_004059"
     CDS             7893..8951
                     /gene="modC"
                     /locus_tag="LGJ20_004059"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBK2388065.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(8952..9770)
                     /gene="ybhA"
                     /locus_tag="LGJ20_004060"
     CDS             complement(8952..9770)
                     /gene="ybhA"
                     /locus_tag="LGJ20_004060"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBK2388066.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            9925..10920
                     /gene="pgl"
                     /locus_tag="LGJ20_004061"
     CDS             9925..10920
                     /gene="pgl"
                     /locus_tag="LGJ20_004061"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBK2388067.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(10961..11914)
                     /locus_tag="LGJ20_004062"
     CDS             complement(10961..11914)
                     /locus_tag="LGJ20_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2388068.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            12098..>12618
                     /locus_tag="LGJ20_004063"
     CDS             12098..>12618
                     /locus_tag="LGJ20_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HBK2388069.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKV"
BASE COUNT         2948 a         3159 c         3334 g         3177 t
ORIGIN      
        1 gggctggcac agccgagcag cgcctcgcgg acgctgccat cggaaagcgg aatacgggcg
       61 gaaagtaaag tcgcacccca gtccatcagc gtgactacca tccctgcgtt gttacgcaaa
      121 gttaacagtc ggtacggctg accatcgggt gccagtgcgg gagtttcgtt cagcactgtc
      181 ctgctccttg tgatggttta caaacgtaaa aagtctcttt aatacctgtt tttgcttcat
      241 attgttcagc gacagcttgc tgtacggcag gcaccagctc ttccgggatc agcgcgacga
      301 tacagccgcc aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca atcacagctt
      361 tgacgatttc taccagagtg tcaatttgcg gcacggtgat ttcgaaatca tcgcgcatag
      421 aggcatgaga ttccgccatc aactcgccca tacgtttcag gtcgccttgc tccagcgcgc
      481 tggcagcttc aacggtgcgg gcgttttcag tcaggatatg acgcacgcgt ttcgccacga
      541 ttgggtccag ctcatgtgca acagcgttga actcttcaat ggtgacatcg cgcagagctg
      601 gctgctggaa gaaacgcgca ccggtttcgc actgttcacg acgggtgttg tattcgctgc
      661 caaccagggt acgtttgaag ttactgttga tgatgacgac agccacacct ttcggcatgg
      721 aaactgcttt agtccccagt gagcggcagt caatcagcaa ggcatgatct ttcttgccga
      781 gtgcggaaat tagctgatcc atgatcccgc agttacagcc aacaaactgg ttttctgctt
      841 cctgaccgtt aagcgcgatt tgtgcgccgt ccagcggcag atgataaagc tgctgcaata
      901 cggttccgac cgcgacttcc agtgaagcgg aagaacttaa cccggcaccc tgcggcacat
      961 tgccgctgat caccatgtcc acgccgccga agctgttgtt acgcagttgc agatgtttca
     1021 ccacgccacg aacgtagtta gcccattgat agttttcatg tgcgacaatg ggcgcatcga
     1081 gggaaaactc gtcgagctga ttttcataat cggctgccat cacgcgaact ttacggtcat
     1141 cgcgtggtgc acaactgatc acggtttgat aatcaatcgc gcagggcaga acgaaaccgt
     1201 cgttgtagtc ggtgtgttca ccaatcaaat tcacgcggcc aggcgcctga atggtgtgag
     1261 tggcagggta gccaaatgcg ttggcaaaca gagattgtgt tttttctttc agactcattt
     1321 cttacactcc gaattcgcga aaatggatat cgctgactgc gcgcaaacgc tctgctgcct
     1381 gttctgcggt caggtctcgc tgggtctctg ccagcatttc ataaccaacc ataaatttac
     1441 gtacggtggc ggagcgcaac agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat
     1501 tctcttcgcc attaaatggt gcgccgtgcc agcccataga gtaggggaag gaacactgga
     1561 agaggttgtc ataacgactg gtcagctttt tcaacgccag cgccaaatcg ctgcgctggg
     1621 cgtcggtcaa atcggtgatc cgcaaaacgt gggctttggg cagcagtagc gtttcgaacg
     1681 gccaggcagc ccagtaaggc acaacggcta accagtgttc ggtttcgaca acggtacggc
     1741 taccgtctgc cagctcgcgc tgaacataat ccaccagcat tggtgatttc tgttcggcaa
     1801 aatattcttt ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc
     1861 aaatctgtcc gtgcggatgc gggttagagc agcccatcgc cgcacctttg ttttcaaaga
     1921 cctgcaccca cgggtacgtt tttcccagct ctgcggtttg ctcctgccag gttttgacga
     1981 tttccgtcaa tgccgcaacg ctcagttctg gcaacgtttt actgtgatcc ggtgaaaagc
     2041 agatcacccg gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg ttgctttctg
     2101 gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg taagtcctgg
     2161 tgtaatcggg gtttttatcg cctgtcaccc gcacattacc tgcgcagagg aagcaatctg
     2221 gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg cgccccctgc caggggcgct
     2281 tagcgcggtg cggtgaaacc agaatccatt gcccggtgag cgggttgtag cggcgatgtg
     2341 gatgatcaac gggattaaat tgcgtcatgg tcgttcctta atcgggatat ccctgtggat
     2401 ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc
     2461 agttcagttc acggtcggct ttgctggcgt ccgcccagta ggccggaagg tcgccctcgc
     2521 gacgcggtgc aaaatgataa ttaaccggtt tgccgcaggc tttgctgaag gcattaacca
     2581 cgtccagcac gctgttgcct acgccagcgc cgaggttgta gatgtgtacg cctggcttgt
     2641 tcgccagttt ttccatcgcc acgacgtgac cgtccgccag atccattacg tggatgtaat
     2701 cgcgtacgcc agtaccatct tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc
     2761 gacggcctac agcaacctgg gcgatgtatg gcatcaggtt attcggaatg ccttgcggat
     2821 cttcgcccat atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc agcagggcaa
     2881 tgctccagtc cggctgggct ttttgcagat cggtgaggat ctgttccacc atcagcttgc
     2941 ttttgccgta agggctttgt ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg
     3001 gctgatcgcc ataaacggtg gcggaggagc taaaaataaa gtttttgacg ttagcggcgc
     3061 gcatggcgct aatcaggcgc agagtgccgt tgacattgtt gtcgtaatat tccagcggtt
     3121 tttgtaccga ttcgccgacg gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt
     3181 gatcgtgcag tatctcggtc atcaacgctt cgttacgaat atcgccttca acaaacgttg
     3241 gatgtttgcc gcctaaacgc tcgataacag gcagtacgct gcgcttactg ttacagaggt
     3301 tatcaagaat gatgacatca tgaccgtttt gcagtaattg cacacaggta tgacttccaa
     3361 tgtaaccgct accaccggta accagaactc tcataattcg ctccattagg cttatggtat
     3421 gaaataacca tagcataaca aagatgcgaa aagtgtgaca tggaataaat tagtggaatc
     3481 gtttacacaa gaatttagcc gttttttatg cgcgattaag tgattataaa acagagggtt
     3541 tatgaatgat tgcgcttttt atctgaaaaa agaagcggtt tcatgcctgc atgcgtcgaa
     3601 ccgttggccg gagagggcgc ttaggccgcc tccggcaagg tcagcactac cgactcaata
     3661 tatttttgtc agcacatagc gatagagtcc accgtccggc acgaactcaa ggcgatgggt
     3721 aatacaggca ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg tttcaccttc
     3781 gctaatcagc acatcaacaa aacggcggat aagctggcga ttgagcggat caagcccctg
     3841 tagtggttca tcgagaataa gcaacgtcgg atgtttcacc agtgcgcgga cgatcagcgc
     3901 cagacgctgc tgtccccagg aaagactatg gaacggagcg tcagccgtac gtttatcaat
     3961 gccgagaata tccagccact gctgcaccag tttttgctgg cgatccgaaa cggcctgata
     4021 aatgccaatc gaatcaaaat agccagaaag aatcacatta cgcacggtag tgctgacccg
     4081 gtaatccaga tgcaaactac tgctgacgta accgatatgc tttttgatat cccagatggt
     4141 ttcgccgctg ccgcgacgtc ggccgaaaag cgtcaaatcg ttgctgtaac cttgcggatg
     4201 atcgccagta atcaggctta ataacgtcga ttttcccgca ccatttggcc cgacaatttg
     4261 ccagtgctcg ccaggattca cctgccagct aaggttatta agaatggggc gatcgttata
     4321 agaaaccacg ccattattca gcacaatgcg cggttcgttg gtgggtaagg cgtgacgtgc
     4381 tgaaggttca tccggctccg gcagttgcac accttcaagc tgttcgctat gcgccagttg
     4441 cgcgacgagt gcttgctgga gcagttcctc tttagcgccc gtttccgcta acgtacaatc
     4501 cgccagcacg ccagcaaact ggacaaattc cgggatctca tcgaagcgat tgagcaccag
     4561 taccagagta ataccggact gatgtaacga ggcgagtcgc tcagccagct gctgacgtga
     4621 ggcaacatcc aggccatcga acggctcatc aagaatcaac aagtcaggct ccgacatcag
     4681 cgcctgacac agcagggttt ttcgcgtctc gccagtggaa aggtatttaa agcgtcggtc
     4741 gaggagggcg gtaataccga actgctgcgc cagttgcatg caacgcggtg catccttaac
     4801 ttcatcctga atgatctcag ccgtagtgcg tccggtgtca tcttcgccag ggccgagcat
     4861 atcggtgtta ttccgctgcc attcgtcgct gacgagcttt tgcaattgct cgaaggagag
     4921 acgagtgatg tgggaaaact ggctttgccg ttcacctttc aaaagcggaa gttcccccgc
     4981 cagcgcacgg gccagcgccg actttccact tccattcgaa ccgacaaacg cccaactatc
     5041 acccgcgttt aacgttagct gaggcaattg cagcgttttt gtgtcgctaa gacgaaacgt
     5101 gccttgcaaa atttgcaacg atgacatttt atatcccatt ttgtgcagcg attactgaca
     5161 gggatacgtg tttcataaca aattgtcaac acgcttagca tagcgtggcg ataatcacgc
     5221 tgtcggcatt aaagtaggcc gtgacattct gtccttgctg aagagaagtc gcttcattaa
     5281 ccggcactgt ggcgcacagt gtttgcccgt cgggtagcgc cattaatact tcgcactgct
     5341 ctgcgccgcg ctcaatatga ctaataatac ccggtaattg gttgtcggcg ttttgtgcga
     5401 ccgcttcgtc ctgagtaatg cctacccacg gcgcttttag caatatcaac acttctttgc
     5461 cttcatccag ccccagacgt gcgccgcttt gtgcggtaat tgcgactttc aggcgtgttt
     5521 ttccgtcagc cagtaagaca tcaacatgct gttgaacgtc atcatgatcg cgggcggtga
     5581 tggtgccgaa ccactggtta cgggcgctgg tttgcagtga aaaacgtgag atcgcggcca
     5641 gcaggctgtt cagcggcagg gcgtcatcgt cacttaacac atcaaaggct ttttgctgga
     5701 tttgcgccag taagtcatag agctgaatca gtcgctgacc atagcgggtc agtactgcgc
     5761 cgccgccacc tttaccgcct gttgcgcgct cgaccagaat atgctcactt aactgattca
     5821 tctcgttaat ggcatcccag gcgcttttat agctgatacc ggcatctttc gctccctggc
     5881 taatggaacc ggaaagcgca atgtgtttta gtagcgaaat gcggcgcggg tcggcgaata
     5941 atttttgttg gagcttaagg gtgagaagga tttcggcctg cataacaatg tcctggcaaa
     6001 agtcttattg tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa ggggagtgct
     6061 tttctgtgct tagcggttag aatagtctca tgactatatc tggagttgac catgttagag
     6121 ttattaaaaa gtctggtatt cgccgtaatc atggtacctg tcgtgatggc catcatcctg
     6181 ggtctgattt acggtcttgg tgaagtattc aacatctttt ctggtgttgg taaaaaagac
     6241 cagcccggac aaaatcattg attccctgaa tgcccgctta gtcgggcatt ttctttttct
     6301 caacttcctg cttttcctgc cgatattttt tcttatctac ctcacaaagg ttagtaataa
     6361 ctgctgggaa aattccgagt tagtcgttat attgtcgcct acataacgtt acattaaggg
     6421 gttaccaatg gctcgtaaat ggttgaactt gtttgccggg gcggcactct ctttcgctgt
     6481 tgctggcaat gcactggcag atgaagggaa aatcacggtg ttcgccgccg catcactgac
     6541 taacgcaatg caggacattg ctacgcagta taaaaaagag aaaggcgtgg atgtggtttc
     6601 ttctttcgct tcgtcatcta ctctcgcccg tcagattgaa gcgggtgcgc ctgcggatct
     6661 gtttatttct gccgatcaga aatggatgga ttatgcagtt gataaaaaag cgatcgatac
     6721 agctacgcgt cagacactgc tcggcaatag cctggtcgtt gtagcaccga aagccagcga
     6781 gcagaaagat ttcatcatcg acagcaaaac taactggact tcgctgctga atggcggtcg
     6841 cctcgcggtt ggcgatccgg aacatgtccc tgctggcatt tatgcaaaag aagcactgca
     6901 aaaactgggc gcatgggata cgctctcccc gaaactggct ccggcggaag atgttcgtgg
     6961 cgcgctggcg ttggtcgaac gcaatgaagc gccgttgggc attgtttacg gttctgacgc
     7021 agttgccagc aaaggggtaa aagtggttgc caccttcccg gaagattcac ataaaaaagt
     7081 ggaatatccg gttgctgttg tggaagggca taacaatgcg acagtgaaag cattttatga
     7141 ttatctgaag ggaccgcagg ctgcggaaat ctttaaacgt tacggattta caaccaagta
     7201 atgatactga ccgatccaga atggcaggca gttttattaa gcctgaaagt ttcttccctg
     7261 gctgtgctgt ttagcctgcc gtttgggatc ttttttgcct ggttactggt gcgttgcacg
     7321 tttccgggca aagctttgct cgacagcgta ctgcatctac cgctggtgtt accgcccgtg
     7381 gtcgtcggtt acttattatt agtttcgatg ggacggcgcg gatttatcgg tgaacgtctg
     7441 tatgactggt ttggtattac cttcgccttt agctggcgcg gcgcggttct cgctgccgcc
     7501 gtcatgtcgt ttccgctaat ggtgcgggca attcgtctgg cgctggaagg ggttgatgtc
     7561 aaactggaac aggccgcaag aacactgggg gccgggcgct ggcgcgtttt ctttactatc
     7621 acgttaccgc tgaccttacc gggaattatt gttggtacgg tactggcttt tgctcgttct
     7681 ctcggtgaat ttggcgcaac catcaccttt gtgtcgaaca ttcctggtga aacgcgaacc
     7741 attccttctg ccatgtatac cctgattcag acccccggcg gggaaagtgg cgcagcgaga
     7801 ctgtgcatta tttctattgc gctggcgatg atctccctgt tgatttcaga atggctggcc
     7861 agaatcagcc gtgaacgggc ggggcgctaa tcatgctgga actgaatttt tcccagacct
     7921 tgggcaacca ttgcctgact attaatgaaa cgctgcccgc caatggcatc actgctatct
     7981 ttggcgtctc cggtgccgga aaaacttcgc tgattaacgc catcagtgga ctgacgcgcc
     8041 cgcaaaaagg gaggattgtc ctcaatggtc gggtactaaa tgatgccgaa aaaggtatct
     8101 gcctgacgcc agaaaaacgt cgcgttggct atgtttttca ggatgcacgg ctgttcccgc
     8161 attacaaagt gcgtggcaat ttgcgctacg gcatggcgaa aagcatggtc gatcagttcg
     8221 ataagctggt ggcgctttta ggcattgaac cgttgcttga ccgtttaccg ggcagcctgt
     8281 ccggaggcga aaaacagcgc gtggcgattg gtcgggcttt gctgacggct ccggaattgc
     8341 tgttgctgga tgaaccgctg gcgtcactgg atattccgcg taaacgcgaa ctgttgcctt
     8401 atctgcaacg tctgacgcgg gaaatcaaca ttccgatgtt gtatgtcagc cattcgctgg
     8461 atgagatcct ccatctggca gacagagtga tggtactgga aaacggtcag gtgaaagcct
     8521 ttggcgcgct ggaagaagtc tggggtagta gcgtgatgaa tccgtggctg ccgaaagagc
     8581 aacaaagtag cattctgaaa gtgacggtac ttgagcatca tccgcattac gcgatgactg
     8641 cgctggcgct gggcgatcag catttgtggg tcaataagct ggacgaaccg ctgcaagctg
     8701 cgctacgtat ccgcattcag gcttccgatg tttctctggt tttacaaccg ccgcagcaaa
     8761 ccagcattcg taacgtattg cgggcaaaag ttgttaatag ttatgacgac aacggccagg
     8821 tggaagtgga gctggaagtc ggcggtaaaa cgctgtgggc gcgtatcagc ccgtgggcca
     8881 gggatgaact ggcgatcaaa cctggcctgt ggctgtacgc gcaaattaaa agtgtgtcga
     8941 taaccgcctg attaaatcag gtggctataa atgaactggg caatgctgtc ggtggtgtta
     9001 tcaccaatca caatgttggc gcgcgctttt accgcgtcat cggcgttgcc catcgccacg
     9061 cctgtaccag cggcttccag catactgata tcattaaagt tatcgccgaa tgccacgacg
     9121 ttttccatcg accaaccttg cgcctcaacc catttcgtca aacgtttacc tttgctgttg
     9181 ccgccgcgtg caatatcaac ctgatcgtgc caggaccatt cacactccag tcccagttca
     9241 tgttcgacat gcttaccaaa atgctgcaat tgcggcaggt catcgtgcgt cagggcgaac
     9301 ttccatacgg cgttaacttg ttgcgccgtt tcagccagag aagcgacttg tgtgaaagtc
     9361 ggacgctgtt ccggcggcag ggtttgcgcc cagttagacg tgcgaatgac atgcccggtc
     9421 gggtgctcat agaccattgc atcatcgaca tacatcagac cgtgaatgtg gtgttcattc
     9481 agcatctcaa tgagttgcag ggctttatta acgggcattg ggtccgcttc cagcacggtt
     9541 tttgcatgat aatcatacaa ataggtgcca ttacagcaaa tagcaggtgt atccagcgcc
     9601 agcgcctgat aaaaaggatg aatagcgacg tgatggcgac ctgtgacgat gattaattga
     9661 tagcctgctt cgcgagcgcg ggccagggct tctatcgatg aaggaagcag ggtctttttc
     9721 ggggtcaata aggtgccgtc taagtcgaga gcaatcacgc gtgtggtcat ggcgtattcc
     9781 agattaaggt taagaatttt cgtctgcgcg aatggtacac cgataccact atcggacaaa
     9841 attctgcgtt ttaattcagc attcaccgcc aaaagcgact aattttagct gttacagtca
     9901 gttgctaaat gcaaaggagc attcatgaag caaacagttt atatcgccag ccctgagagc
     9961 cagcaaattc acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc
    10021 gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt
    10081 ggtgttcgcc ctgagtttcg cgtcctggcg tatcgtattg ccccggacga tggcgcactg
    10141 acctttgccg cagagtctgc gctgccgggt agtccgacgc atatttccac cgatcaccag
    10201 gggcagtttg tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa
    10261 gatggcctgc cagtgggcgt cgtcgatgtg gtcgaggggc tggacggttg ccattccgcc
    10321 aatatctcac cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc
    10381 ctgtttacgg tcagcgatga tggtcatctc gtggcgcagg accctgcgga agtgaccacc
    10441 gttgaagggg ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc
    10501 gtcaatgagt taaacagctc agtggatgtc tgggaactga aagatccgca cggtaatatc
    10561 gaatgtgtcc agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct
    10621 gatattcata tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagcctg
    10681 attaccgttt tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca
    10741 acggaaaccc agccgcgcgg cttcaatgtt gatcacagcg gcaagtatct gattgccgcc
    10801 gggcaaaaat ctcaccacat ctcggtatac gaaattgttg gcgagcaggg actactgcat
    10861 gaaaaaggcc gttatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa
    10921 ccgctgattt aaccggcgca gtctctcctg cgccggtgta ttaacttatc tcctgtaacg
    10981 catgtctctg gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat
    11041 taatcagcaa ggcaaaaaag cgttcagctg ccggagaaag cacggcatct ttgcggcgaa
    11101 taatccccaa tgtccgacgt ataacgggtt ccaccagtgg gataccaata atcgaagaat
    11161 agggcgcatg gggcattgcc agaccaggaa gcgccgaaat ccccagtcct gcctccacta
    11221 gtcctaatga cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt
    11281 cggctagctg ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt
    11341 tgtaacccac taactcctgc cattctacaa gctgcttttt ggccaatgga tggtcacgcc
    11401 gacaggccag cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga
    11461 cgttgttcat gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat
    11521 tcgtgccttg ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga
    11581 ttgccagcgg taaaaaataa aatactgcgg tcggaataca cgctaacgtc accataccac
    11641 gatgataagc attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt
    11701 cccgcgcctc cgggagcaac cttttccctg cttttgttaa cgtaaccttg cgagttgtac
    11761 gctcaaatag ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag
    11821 gttgcgtaat attgagtaat ttagcggcat tgttaaatga actggactct gccagtatga
    11881 caaatgcctt catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg
    11941 attttgggtg gctggccgtt aaaaatttta actgcattta gccaacttaa attaatgaaa
    12001 aaatgttatt aatcgttgag ctaaagtcat tagagatgct ttgcccttaa tgtaaccata
    12061 tcgcaataag ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat
    12121 gatgcgaggt ggaacctcga ggggcgcgtt cttgttagcg gaacatttac ccgaagatca
    12181 aacgcagcgc gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga
    12241 cggtattggc ggcggtaatc cgctgaccag taaagtcgcc attattagcc gttccagcga
    12301 tccgcgtgct gatgtcgatt atctgtttgc tcaagttatt gtccatgagc aacgtgtcga
    12361 taccacgcct aattgtggca atatgctgtc tggtgttggg gcattcgcca ttgaaaatgg
    12421 tttgattgca gcgacttcac cagttacccg cgtacgtatc cgcaacgtca atactggtac
    12481 gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagcgccag
    12541 aattgacggc gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgccgg
    12601 aaccaaaacc ggaaaagt
//
LOCUS       DAFATA010000119        12478 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFATA010000119 DAFATA010000000
VERSION     DAFATA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.120"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..615)
                     /locus_tag="LGJ20_004064"
     CDS             complement(<1..615)
                     /locus_tag="LGJ20_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBK2388070.1"
                     /translation="MSATKLTRREQRAHAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRD"
     regulatory      complement(695..795)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(847..1323)
                     /gene="rsd"
                     /locus_tag="LGJ20_004065"
     CDS             complement(847..1323)
                     /gene="rsd"
                     /locus_tag="LGJ20_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBK2388071.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            1418..2191
                     /gene="nudC"
                     /locus_tag="LGJ20_004066"
     CDS             1418..2191
                     /gene="nudC"
                     /locus_tag="LGJ20_004066"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBK2388072.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            2231..3295
                     /gene="hemE"
                     /locus_tag="LGJ20_004067"
     CDS             2231..3295
                     /gene="hemE"
                     /locus_tag="LGJ20_004067"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBK2388073.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            3305..3976
                     /gene="nfi"
                     /locus_tag="LGJ20_004068"
     CDS             3305..3976
                     /gene="nfi"
                     /locus_tag="LGJ20_004068"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBK2388074.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            4019..4609
                     /locus_tag="LGJ20_004069"
     CDS             4019..4609
                     /locus_tag="LGJ20_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBK2388075.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            4796..5068
                     /gene="hupA"
                     /locus_tag="LGJ20_004070"
     CDS             4796..5068
                     /gene="hupA"
                     /locus_tag="LGJ20_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBK2388076.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            5081..5776
                     /locus_tag="LGJ20_004071"
     CDS             5081..5776
                     /locus_tag="LGJ20_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBK2388077.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(5778..6203)
                     /gene="zraP"
                     /locus_tag="LGJ20_004072"
     CDS             complement(5778..6203)
                     /gene="zraP"
                     /locus_tag="LGJ20_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBK2388078.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            6441..7817
                     /gene="zraS"
                     /locus_tag="LGJ20_004073"
     CDS             6441..7817
                     /gene="zraS"
                     /locus_tag="LGJ20_004073"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBK2388079.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            7814..9139
                     /gene="zraR"
                     /locus_tag="LGJ20_004074"
     CDS             7814..9139
                     /gene="zraR"
                     /locus_tag="LGJ20_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBK2388080.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(9136..10425)
                     /gene="purD"
                     /locus_tag="LGJ20_004075"
     CDS             complement(9136..10425)
                     /gene="purD"
                     /locus_tag="LGJ20_004075"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK2388081.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(10437..12026)
                     /gene="purH"
                     /locus_tag="LGJ20_004076"
     CDS             complement(10437..12026)
                     /gene="purH"
                     /locus_tag="LGJ20_004076"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK2388082.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAESAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         2843 a         3253 c         3497 g         2885 t
ORIGIN      
        1 atcacggaca aattccgcag tgatattttc cggcagacgt gcgccaaagc tcattcccgg
       61 atgctggtgg cgtaaaactt cgctacgtat gcgctcgcgg cccatattct cgcggatggc
      121 gatgaattcc atttccggcg tgatgatgcc ctggcgggcg tagtgcagtt gggtgacacg
      181 gcgtcctgct ttggcgcgtt ttggtgttag tacgccgcta aaacgcagtt cgtcgaggcc
      241 atcatctgcc agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc
      301 gccgcgcgca tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc
      361 aatctgcgga tcaccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc
      421 gtactgcggc tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat
      481 cggcacgcga acgccggggt gcgtgccagt gatataaata cgttttgagt tgggaaaggc
      541 ggtgccttcc agggtgtcga taaaatgttg ggcgtgggcg cgctgttcgc ggcgggtcag
      601 ttttgttgca gacatagctc attccaaaag ttaaggacgt ggcttgtcag acgacggatg
      661 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
      721 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
      781 ctccgacaag aaattagccc cgcaaatggg gcattgaatg taaattacgc gttaacagca
      841 cagaactcaa gcaggatgtt tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc
      901 ttccagcaca aagcgcgctt ccagcgcctc gccgatgtca gataaaacct gttgaaactc
      961 aaggtaatta tcatgatcga tagcggtttc caggctggaa tcgtagtaat ccataatctg
     1021 ttgagtattg gcttcaagtt gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc
     1081 ttccagctta tgaagaatgc gctcataaat actgaaatgc ccggcagaca agtaatcgac
     1141 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
     1201 aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
     1261 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
     1321 catgattgac tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg
     1381 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
     1441 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
     1501 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
     1561 atggcagggg gaacctgttt ggttagtaca acagcagcgg cgtcacgata tggggtcggt
     1621 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgag gcgtacaact
     1681 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccgag
     1741 caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
     1801 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
     1861 ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggcg aaaccctcga
     1921 gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa acttgcgtta
     1981 cgtgacttct cagccgtggc cgtttcctca gtctttaatg accgcgttta tggcggaata
     2041 tgacagcggc gacatcgtga tcgacccgaa agaattgctc gaggcgaact ggtatcgcta
     2101 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
     2161 ggtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
     2221 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
     2281 cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
     2341 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg
     2401 cgaagtgact ttgcaaccgc tgcgtcgcta cccgctggat gcggcgatcc tcttttccga
     2461 tatcctcacc gtgccggacg cgatggggtt agggctctat tttgaagccg gagaaggtcc
     2521 gcgttttacc tcgccagtca cctgcaaagc cgacgtcgat aaactgccaa ttccggaccc
     2581 ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
     2641 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcaacct acatggtgga
     2701 aggcggcagc agcaaagcct tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc
     2761 gctgcacgct ctactcgata aactggcgaa aagcgtcact ttgtatctga atgcgcagat
     2821 taaagccggt gctcaggctg tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
     2881 cgattatcaa cagttctcgc tttattacat gcataaaatt gttgatggtt tactgcgtga
     2941 aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa ggcggcggac agtggctgga
     3001 agccatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
     3061 tgcgcgccgc cgtgtgggca ataaagtcgc gttgcagggt aatatggatc cgtcgatgct
     3121 gtacgcgccg cctgcccgca ttgaagaaga agtagcgact atacttgcag gtttcggtca
     3181 cggcgaaggt catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
     3241 tgctggcgtg ttcgtggagg cagtgcatcg actgtctgaa cagtatcacc gctaaggagt
     3301 gattatggat ctcgcgtcat tacgcgctca acaaatcgaa ctggcttctt ctgtgatccg
     3361 cgaggatcga ctcgataaag atccaccgga tctgatcgcc ggagccgatg tcgggtttga
     3421 gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatatccct cgcttgagct
     3481 ggtggagtat aaagttgccc gcatcgccac caccatgcct tacattccag gttttctttc
     3541 cttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa agccggattt
     3601 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtcg ccagccattt
     3661 tggtttgatg gtggatgtgc caacgatcgg cgtggcgaaa aaacggctct gcggtaaatt
     3721 cgaaccgctc tccagcgaac cgggcgcgct ggccccactg atggataaag gcgagcagct
     3781 ggcctgggtc tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg
     3841 ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc
     3901 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
     3961 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
     4021 gttacaaaac ccaattcatc tgcgtctgga gcgcctggaa agctggcagc atgtcacttt
     4081 catggcttgc ttatgcgaac gcatgtaccc caattacgcc atgttctgcc agcaaaccgg
     4141 ttttggtgat gggcaaattt accgtcgcat tctcgatctc atctgggaaa cgctgaccgt
     4201 taaagacgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
     4261 agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcctgcg tggcgttaag
     4321 tgaactggtc cattcgcgtt tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa
     4381 gacctccatc acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
     4441 tgaagagctt aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
     4501 ccgactttta gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
     4561 cctgcgtgag gcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac
     4621 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
     4681 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
     4741 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
     4801 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
     4861 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
     4921 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
     4981 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
     5041 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
     5101 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggctg gcgctctgct
     5161 tctcactgcc tgtagtcata actcttcact tcctcccttt accgccagtg gatttgctga
     5221 agaccagggc gcagtacgca tctggcgaaa agacagcggc gataatgtgc atctgcttgc
     5281 cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga gagtatcgct ggcagggcga
     5341 taacctcacg ctcatcaata tcaatgttta cagcaaaccg ccggtgaaca ttcgcgcgcg
     5401 ttttgacgat cgcggtgatc tgagctttat gcaacgtgaa tccgatgggg aaaagcagca
     5461 gctttctaac gaccaaatcg atttataccg ttatcgtgct gatcagatcc gccagattag
     5521 cgatgcctta cgtcagggga gagtcgtgct gcgccagggg cgctggcatg cgatggaaca
     5581 gaccgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc
     5641 gcatatcgag cgccgccaga gccgctcttc tgttgatgtc agcgtggcat ggctggaagc
     5701 gcccgaaggt tcgcaattac tgttagtggc aaactctgat ttctgtcgct ggcaacccaa
     5761 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg
     5821 ccgtagccca tgcccattcc ggctccgcgc ggaatacccg cttcagccat cgcgatatct
     5881 cgtttcaccc gtaactcatc taacgactga cgcaaattct ccatctcttt ggcgaccgcg
     5941 ttaattttgc tgctatccgg tgggttcgcg gctaacaggg cattgtattc ataacgcttc
     6001 gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg gattttctgc
     6061 cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt gctgccacat accgtgtccg
     6121 ccgtgagcaa atgcagatgt cgatcccatc gccattgctg aaagcgccat cattaccagg
     6181 gcaattttcg tgttccgttt catggttaat cctccagtgg ttgtcttact tcgggtattg
     6241 catcttccgt gccaacgatg aaacgctgat atgacgggta atctggcatg ataaacgagt
     6301 aaaaatgact cgcctgctgc gggtagcgag tcatttttac tcattgaaac ttaagccttt
     6361 gtgttacagc gcagggtaag cgctgataaa agatggcatg atttctgctg tcagaaaggg
     6421 atgagcaggc aaagaagaag atgcgtttta tgcaacgttc taaagactcc ttagctaaat
     6481 ggttaagcgc gatcctcccc gtggtcattg ttgggctggt agggttgttt gcggtgacgg
     6541 tgattcgcga ttatgggcgc gagactgccg ccgccagaca aacgctgctg gaaaaaggca
     6601 gtgtacttat tcgcgctctt gaatccggct cgcgcgtcgg catggggatg cgcatgcatc
     6661 atgcgcagca gcaggcctta ctggaagaaa tggccgggca gcctggagta cgttggtttg
     6721 cggtcacgga tgaacaagga acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac
     6781 agctttattc cccgcaggaa atgcagcagt tacatcctgg agatgaagaa gcgtggcggc
     6841 ggatcgatag cgcagacggc gagcctgttc tggaaattta tcgccagttt caaccgatgt
     6901 ttgctgctgg aatgcaccgg atgcgccata tgcagcagta tgccgcgaca ccacaagcaa
     6961 ttttcatcgc tttcgacgcc agtaatattg tgagtgccga agatcgtgag cagagaaaca
     7021 ccctgattat cctcttcgcc ctggcgacgg tcttgctggc aagcgtgttg tcattcttct
     7081 ggtatcgccg ctatctgcgc tcgcgccagc tgttgcagga tgaaatgaag cgcaaagaga
     7141 agctggtagc tctggggcat cttgcggcag gcgttgccca cgaaatccgt aatccacttt
     7201 cctcaattaa agggctggcg aaatactttg ccgaacgcgc gccagcaggg ggagaagcgc
     7261 atcaattggc gcaggtgatg gcgaaagaag ccgaccgttt aaaccgcgtg gtaagcgagt
     7321 tgctggaact ggttaagcca acgcatctgg ctttgcaggc ggtggatctc aacacgctga
     7381 ttaaccactc attacagctg gtaagccagg atgcaaacag ccgggagatc cagttacgct
     7441 ttaccgccaa cgacacatta ccggaaattc aggccgatcc ggacaggctg actcaggtcc
     7501 tgttgaatct ctatctcaat gctattcagg cgattggtca gcatggcgtg attagcgtga
     7561 cggtcagcga aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg
     7621 cggcagatca gcttgaagcc atcttcactc cgtacttcac caccaaagcc gaaggcaccg
     7681 gattggggct ggcggtcgtg cataatattg ttgaacaaca cggtggtaca attcaggtcg
     7741 caagccagga gggaaaaggc tcaacgttca ccctctggct tccggtcaat attacgcgta
     7801 aggacccaca aggatgacgc acgataatat cgatattctg gtggtggatg atgacattag
     7861 ccactgcact attttgcagg ctttactgcg cggctggggc tataacgtcg cgctggcgaa
     7921 cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga
     7981 tgtgcgaatg gcggagatgg acggcatcgc cacgctgaaa gagatcaaag cgttaaaccc
     8041 ggcaattccg gtgctgatta tgactgcgta ctccagcgtc gagacggcgg tagaggcact
     8101 gaaaactggg gcgctggatt atctcatcaa gccgctggat ttcgataacc tacaggcgac
     8161 gctggaaaag gcgctcgcgc atacgcacag tattgatgct gaaacacctg cggtgactgc
     8221 cagccagttc ggtatggtcg gtaaaagctc ggcgatgcaa cacctgctca gtgaaatcgc
     8281 cctcgtcgcg ccatcggaag ccacggtact gatccacggc gattccggca ccggtaaaga
     8341 gctggtcgcc agggcgattc acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct
     8401 caactgtgcg gcactcaacg aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg
     8461 ggcgtttact ggagccgata aacgccggga agggcgcttt gttgaggcgg acggcggcac
     8521 gctgtttctc gatgaaattg gcgatatctc gccgatgatg caggtgcgtc tgctgcgtgc
     8581 gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag actatctcgg ttgatgtccg
     8641 gctgattgcg gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca
     8701 ggatctctac tatcgcctga atgtggtggc gattgaagta ccatcgctgc ggcaacggcg
     8761 ggaagatatc cctctgctgg ctggccattt tctgcagcgc tttgccgagc gtaatcgcaa
     8821 ggcggtaaaa ggttttacgc cccaggcgat ggatctgttg atccactacg actggccggg
     8881 aaatattcgt gaactggaaa acgcggtgga acgggcggtg gtgctgctga ccggggaata
     8941 tatttccgaa cgcgagctgc cgctggcgat tgccggtacg ccgatcccgc tggggcaaag
     9001 tcaggatatt cagccgctgg tagaagtcga aaaagaagtg attctggcgg cactggagaa
     9061 aacgggcggc aacaaaaccg aggccgcccg tcagttaggg atcacgcgca aaacgctatt
     9121 ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg cgccagccaa tatctttccg
     9181 gcagaagcag tcgtcccagt gaatatcggt cattaaggca taagcgcgtt tctgcgcttc
     9241 tgccacggta tgacccagcg cggtgacgca cagtacgcgc ccgccgctgg tcactacctg
     9301 ctcgttatcc gccagtttcg tgcccgcgtg gaacactttg ccgtcagcca cttcttccag
     9361 cggcaggccg tggatcacat cgccggtgcg gtaatcgccc ggatatccac ccgcagccat
     9421 caccacgccg agagaagcgc gttcatccca ctcggatgtt ttctcgtcca gcttcccttc
     9481 acaggctgcc aggcagagct caaccagatc ggacttcatg cgcagcataa tcggctgggt
     9541 ttccggatcg ccaaagcggc agttaaattc gataaccttc ggattgccct gtttgtcgat
     9601 catcaggccc gcgtagagaa aaccggtgta ggtgttgcct tccgccgcca tgcctttcac
     9661 ggttggccag atgatacgtt ccatggtgcg ctgatgaact tcatcggtta ctaccggcgc
     9721 gggggagtaa gcgcccatcc cgcccgtgtt tggtccggta tctttatcgc ctacgcgttt
     9781 gtgatcctgg ctggtagcca tcggcaacac atgctcgccg tccaccatca cgataaagct
     9841 cgcttcttcg ccatcgagga actcttcgat aacgatgcga tgacccgcgt cgccaaaagc
     9901 gttgcccgcc agcatatcgt gaacagccgc ttccgcttct tccagcgtca tcgccacgat
     9961 aacgcctttc ccggcagcca gaccgtccgc tttaatgacg attggcgcgc ctttctcacg
    10021 cagatacgcc agcgcaggtt ctacctcggt gaagttctgg tattccgccg tagggatctt
    10081 atggcgggcc aggaaatctt tggtaaacgc ttttgagcct tccagttggg ccgcacctgc
    10141 ggttgggccg aagattttca gcccggcggc gcggaaggta tcgaccacgc ctttcaccag
    10201 cggcgcttcc gggccgacga tggtcagatc aatcttttcg ttttgtgcga aatccaacag
    10261 cgccgggata tcggtcacgc caatagcaac gttttgcagc gcgggttcca gtgcagtgcc
    10321 tgcattaccc ggagcaacaa aaacagtctc aaccagcggc gactgggccg ctttccaggc
    10381 cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact ttcatctatt gctccattaa
    10441 tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca
    10501 atcacttcgt catcacggat ggaaccgcca ggctggatta cgcaggtcac gcccgcagcg
    10561 gcggcggcat caataccgtc gcggaacggg aagaacgcgt cagaagccat cgaggaacct
    10621 ttcacttcca ggccttcatc ggcagcttta ataccggcga ttttcgcgga gtacacgcgg
    10681 ctcatctggc ccgcgccaat accgatggtc atattgtttt tggcatagac gatagcattg
    10741 gatttcacga acttcgccac tttccagcag aacagcgcat cgcgcagttc ctgttcggtc
    10801 ggctgacgtt gggtgacgac gcgcagttct tccgcaccga ccatccccag gtcacgatcc
    10861 tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac ccggaacacg ctcgccccac
    10921 tgaccgcagg tcagaacgcg tacgttctgc ttggcggcgg tgattttcag ggcttcttcg
    10981 ctggcggaag gcgcaataat cacttcgaca aactgacgag aaatgatggc ctgtgcggat
    11041 tccgcatcca gctcgcggtt aaaggcaatg atgccgccga atgcggaggt tgggtcggtt
    11101 ttgtacgcgc gatcgtaagc atcaagaatg gaattgccga tagctacacc gcaagggttg
    11161 gcgtgcttca caatcacaca tgccggctcg gcgaactctt tcacgcactc cagcgccgca
    11221 tcggtatcgg cgatgttgtt ataagagagg gctttaccct gaacctgggt tgcggtagca
    11281 acggaggctt ctttcacatt ctcttctata tagaaggcag cctgctggtg gctgttctcg
    11341 ccgtaacgca tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg gaagcgacca
    11401 gcggcttctt tgctttcacc gtggtacgcc ggaaccatgc tgccgaagta gttggcaatc
    11461 atgctgtcgt aggcggcagt gtgttcgaag gctttgatgg cgaggtcgaa acgggttgca
    11521 agcgtcagcg atccttcgtt gtcatccatc tctttaataa tggcgtcgta gtcgctgctc
    11581 ttcaccacga ttgcgacatc tttatggttc ttagcggcgg agcgcaccat ggtcgggccg
    11641 ccgatatcga tgttctcaac cgcatcttcc agcgagcaac cttcacgggc cacggtctgg
    11701 gcgaacggat acaggttaac aaccaccata tcgataggct ggatctgatg ttcttccata
    11761 atggcatcgt cctggccgcg acggcccaga atgccaccat gtactttcgg atgcagggtc
    11821 ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc
    11881 agaccttttt ctgctaacag acgggcagtg ccccctgtag acagcagctc cacaccgcgt
    11941 gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt cagaaacact gagcagagcg
    12001 cggcggactg gacgacgttg ttgcatggta aatcccctgg atttgactat tacagagagc
    12061 gttagctgaa tttttcgcga aaaactcagc taacgcccct aacggggcat ccttattttt
    12121 cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga atttttctct ttcaatggtg
    12181 atcacaattt tgacagtggt taccgtggga aaaatacaga aattacattg atgattgtgg
    12241 ataactctgt gcgtaaaaag gtataaagcg gggttttgct ggggaatgca gcagtcagtc
    12301 atttttctgc aatttttcta ttgcggcctg cggagaactc cctataatgc gcctccatcg
    12361 acacggcgga tgtgaatcac ttcacacaaa cagccggttc ggttgaagag aaaaatcctg
    12421 aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc gacagtgc
//
LOCUS       DAFATA010000120        12317 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFATA010000120 DAFATA010000000
VERSION     DAFATA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.121"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1860
                     /locus_tag="LGJ20_004077"
     CDS             <1..1860
                     /locus_tag="LGJ20_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK2388083.1"
                     /translation="EAQRDNLATLKGEFIDGKYMRVHRTIGSTRMDIKIAHARIENKI
                     VNLLEPLATLAWTLGFEYHHGLLEKMWKEILKNHAHDSIGCCCSDKVHREIVARFELA
                     EDMADNLIRFYMRKIADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDR
                     GQPVPYFIRHAREIDPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQP
                     GNVIAAKSDAEGILENAFWQIALNEDGSLQLVDKDSGVRYDRVLQIEESSDDGDEYDY
                     SPAKEEWVITAANAKPQCDIIHEAWQSRAVIRYEMAVPRNLQERSARQSTGRVGVEMV
                     VTLSDNSRRIDVDINLDNQADDHRLRVLVPTPFNTDSVLADTQFGSLTRPVNDSAMNN
                     WQQEGWKEAPVPVWNMLNYAALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVG
                     LLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTP
                     VQCYNKIPWDAMKLNKAGFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAE
                     SATCDATVAFSREVISCSETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            2708..4276
                     /gene="cydA"
                     /locus_tag="LGJ20_004078"
     CDS             2708..4276
                     /gene="cydA"
                     /locus_tag="LGJ20_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBK2388084.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            4292..5431
                     /gene="cydB"
                     /locus_tag="LGJ20_004079"
     CDS             4292..5431
                     /gene="cydB"
                     /locus_tag="LGJ20_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBK2388085.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            5446..5559
                     /gene="cydX"
                     /locus_tag="LGJ20_004080"
     CDS             5446..5559
                     /gene="cydX"
                     /locus_tag="LGJ20_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBK2388086.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            5559..5852
                     /gene="ybgE"
                     /locus_tag="LGJ20_004081"
     CDS             5559..5852
                     /gene="ybgE"
                     /locus_tag="LGJ20_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBK2388087.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            6002..6406
                     /gene="ybgC"
                     /locus_tag="LGJ20_004082"
     CDS             6002..6406
                     /gene="ybgC"
                     /locus_tag="LGJ20_004082"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBK2388088.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            6403..7095
                     /gene="tolQ"
                     /locus_tag="LGJ20_004083"
     CDS             6403..7095
                     /gene="tolQ"
                     /locus_tag="LGJ20_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBK2388089.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            7099..7527
                     /gene="tolR"
                     /locus_tag="LGJ20_004084"
     CDS             7099..7527
                     /gene="tolR"
                     /locus_tag="LGJ20_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBK2388090.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            7592..8857
                     /gene="tolA"
                     /locus_tag="LGJ20_004085"
     CDS             7592..8857
                     /gene="tolA"
                     /locus_tag="LGJ20_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBK2388091.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            8990..10282
                     /gene="tolB"
                     /locus_tag="LGJ20_004086"
     CDS             8990..10282
                     /gene="tolB"
                     /locus_tag="LGJ20_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBK2388092.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            10317..10838
                     /gene="pal"
                     /locus_tag="LGJ20_004087"
     CDS             10317..10838
                     /gene="pal"
                     /locus_tag="LGJ20_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBK2388093.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            10848..11639
                     /gene="cpoB"
                     /locus_tag="LGJ20_004088"
     CDS             10848..11639
                     /gene="cpoB"
                     /locus_tag="LGJ20_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBK2388094.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            11804..11879
                     /locus_tag="LGJ20_004089"
     tRNA            11804..11879
                     /locus_tag="LGJ20_004089"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11837..11839,aa:Lys,seq:ttt)
     gene            12015..12090
                     /locus_tag="LGJ20_004090"
     tRNA            12015..12090
                     /locus_tag="LGJ20_004090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12048..12050,aa:Val,seq:tac)
     gene            12093..12168
                     /locus_tag="LGJ20_004091"
     tRNA            12093..12168
                     /locus_tag="LGJ20_004091"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12126..12128,aa:Lys,seq:ttt)
BASE COUNT         3084 a         2888 c         3297 g         3048 t
ORIGIN      
        1 gaagcgcagc gagataatct ggcaaccctg aaaggggaat ttattgatgg caaatatatg
       61 cgcgtgcatc gcaccatcgg ttctacgcgt atggatatca aaattgccca cgcgcgtatt
      121 gaaaataaga ttgttaatct gctggaaccg ctggcaacac tggcctggac gttgggtttt
      181 gaataccacc acggcttgct ggagaaaatg tggaaagaga tcttaaaaaa tcatgcccac
      241 gacagtatcg gttgttgttg cagtgacaaa gttcatcgcg aaatcgtcgc ccgcttcgaa
      301 ctggctgaag acatggcgga taatctgatt cgtttctaca tgcgcaaaat tgccgacaac
      361 atgccgcaga gcgacgccga caaactcgtc ctgtttaacc tgatgccctg gccgcgtgaa
      421 gaagtgatca acaccactgt gcgcctgcgc gccagccagt ttaatttgcg ggacgatcgc
      481 ggccagcctg tgccgtattt tattcgccat gcccgtgaga tcgatccagg cctaatcgat
      541 cggcaaattg ttcattatgg caattatgat ccctttatgg agttcgacat ccagatcaac
      601 cagattgtcc cttctatggg ctatcgcacg ctttatattg aagcgaatca gcctggcaac
      661 gtgattgcgg caaaaagtga cgctgaaggg attctggaaa atgctttctg gcagattgcg
      721 ctcaatgagg atggttctct gcaactggta gataaagaca gcggtgtgcg ctatgaccgg
      781 gtattgcaaa ttgaagaaag ctctgatgat ggtgatgaat atgactattc acccgcaaaa
      841 gaagagtggg taattaccgc ggcgaacgcg aaaccgcaat gcgatattat tcatgaagcc
      901 tggcagagca gggctgttat ccgttatgaa atggcggtgc cgcgtaatct gcaagaacgc
      961 agcgcaagac aatccactgg cagagtaggg gtggaaatgg ttgtcacttt gagtgataac
     1021 agcaggcgca ttgatgtgga tatcaacctt gataaccagg ctgacgatca tcgtcttcgc
     1081 gtcctggtcc ctacaccttt taacaccgac agtgttctgg cagataccca gtttggttcg
     1141 ctaacgcgcc ccgtgaacga cagtgcaatg aacaactggc agcaagaagg ctggaaagaa
     1201 gcgccggttc cggtatggaa tatgctcaac tatgctgcct tacaggaagg gcgtaacggc
     1261 atggctgtct ttagcgaagg gttacgtgaa tttgaagtca tcggtgaaga gaagaaaacc
     1321 tttgccatta cgttgctgcg tggcgtgggc ttactgggga aagaagatct gcttttaagg
     1381 cctgggcggc cttcgggaat taaaatgcca gtcccggact cacaactacg tggtctgctt
     1441 tcttgtcgcc taagtttatt gagttatacc ggtacgccaa ccgccgctgg tgtagctcag
     1501 caggcgcgag catggctgac tccagtacag tgttacaaca aaatcccatg ggatgcgatg
     1561 aagctcaaca aagccggatt caacgtgccg gaaagttata gtttgttgaa aatgccccca
     1621 gtgggatgcc tgataagcgc acttaagaaa gctgaagacc gacaagaagt gattttacgg
     1681 ctgtttaatc cggctgaatc agcaacctgt gatgcgactg ttgctttcag tcgcgaggtg
     1741 atttcttgct cagaaacgat gatggatgaa cacattacca ccgaggaaaa tcaaggttca
     1801 aatctatcgg ggcctttttt acccggccag tcacggacgt tcagttaccg gcttgcctga
     1861 atagcaatca aaccgaagcc acatatgcgc ggccagattg ttgacaaagg gcgctttgtt
     1921 catgccggat acggcatgaa cgctttattc ggtctacaaa agcaggcaaa ttcaatatat
     1981 tgcagagatg atgtagacac tggcaagcgt agcgcatcag gtaattttgc gtttatcttc
     2041 actctcaagc cacgtatatg tggctttatt tttaacaaaa taataaccct gggtgagtta
     2101 attataatat aattataagt taattaaatg ttaatatcgg cgggggtgga tttatgcctt
     2161 tattagtaat cctgaaactc tgcgtcgtat tagccagtga ccaaaaaaag aattaaggtc
     2221 aaccgtgctg tttttgcttc gtctcttttt atctttaatt gccaaccgaa actaatttca
     2281 gccttataac tcacacattt taaacataaa tgtcactaaa gttaccttat tgaaacatga
     2341 ttaacataat ttgtaggaat tgatatttat caatgtataa gtcttggaaa tgggcatcaa
     2401 aaagagataa attgttctcg atcaaattgg ctgaaaggcg gtaatttagc tataaattga
     2461 tcaccgtcga aaaatgcaaa tttgcttcaa caaaaacctg tttattgtaa ggattttgcg
     2521 gcgtaatata tacgtgggat caatttgagt ttttattaac atgtttgcaa cctttcttta
     2581 cgccgttttt gtgtgcattc acatggtatg atgaaagtgt tcaaacaaat ttctattggg
     2641 gcatgcgtgt gaccctttct aacggggttc actctcggag tcttcatgcg atgagcaagg
     2701 agtcatgatg ttagatatag tcgaactgtc gcgcttacag tttgccttga ccgcgatgta
     2761 ccacttcctt tttgtgccac tgacgctcgg tatggcgttc ctgctggcca ttatggaaac
     2821 ggtctacgtc ctctccggca aacagattta taaagatatg accaagttct ggggcaagtt
     2881 gtttggtatc aacttcgctc tgggtgtggc taccggtctg accatggagt tccagttcgg
     2941 gactaactgg tcttactatt cccactatgt aggggatatc ttcggtgcgc cgctggcaat
     3001 cgaaggtctg atggccttct tcctcgaatc cacctttgta ggtctgttct tcttcggttg
     3061 ggatcgtctg ggtaaagttc agcatatgtg tgtcacctgg ctggtggcgc tcggttcaaa
     3121 cctgtccgca ctgtggattc tggttgcgaa cggctggatg caaaacccaa tcgcgtccga
     3181 tttcaacttt gaaactatgc gtatggagat ggtgagcttc tccgagctgg tgcttaaccc
     3241 ggttgctcag gtgaaattcg ttcacactgt agcgtctggt tatgtgactg gcgcgatgtt
     3301 catcctcggt atcagcgcat ggtatatgct gaaaggtcgt gacttcgcct tcgctaaacg
     3361 ctcctttgct atcgctgcca gcttcggtat ggctgctgtt ctgtctgtta ttgttctggg
     3421 tgatgaatcc ggctacgaaa tgggcgacgt gcagaaaacc aaactggctg ctattgaagc
     3481 cgagtgggaa acgcaacctg cgcctgctgc ctttactctg ttcggcattc ctgatcagga
     3541 agaggagacg aacaaatttg cgattcagat cccttacgca ctgggcatca ttgcaacgcg
     3601 ttccgtggat accccggtta tcggcctgaa agagctgatg gtgcagcatg aagaacgcat
     3661 tcgtaacggg atgaaggcgt actctctgct cgaacaactg cgttctggtt ctaccgacca
     3721 ggcggttcgt gaccagttca atagcatgaa gaaagacctc ggttacggtc tgctgctgaa
     3781 acgctatacg ccaaacgtgg ctgatgcgac tgaagcgcag attcaacagg caaccaaaga
     3841 ctccatcccg cgtgtagcgc cgctgtactt tgcgttccgt atcatggtgg cgtgtggctt
     3901 cctgcttctg gcaatcatcg cgctctcttt ctggagtgtc atccgcaacc gcattggcga
     3961 gaaaaaatgg cttctgcgcg ccgcgctgta cggtattccg ctgccgtgga ttgctgtaga
     4021 agcgggctgg ttcgtggctg aatatggccg ccaaccgtgg gctatcggtg aagtgctgcc
     4081 gacagctgtg gcgaactcgt cactgaccgc aggcgatctc atcttctcaa tggtgctgat
     4141 ttgcggcctg tataccctgt tcctggtggc agaattgttc ttaatgttca aatttgcacg
     4201 cctcggccca agcagcctga aaaccggtcg ctatcacttt gagcagtctt ccacgactac
     4261 tcagccggca cgctaagaca ggagtcgtca aatgatcgat tatgaagtat tgcgttttat
     4321 ctggtggctg ctggttggcg ttctgctgat tggttttgca gtcactgacg gtttcgacat
     4381 gggggtgggc atgctcaccc gtttcctcgg tcgtaacgac accgagcgtc gaattatgat
     4441 taactccatt gcaccacact gggacggtaa ccaggtttgg ctgatcaccg cgggcggcgc
     4501 actctttgct gcctggccga tggtctatgc cgctgcgttc tccggcttct atgtggcgat
     4561 gatcctcgtg ctggcgtctt tgttcttccg tccggtcggt tttgactacc gctccaagat
     4621 tgaagaaacc cgctggcgta acatgtggga ctggggcatc ttcattggta gcttcgttcc
     4681 gccgctggta attggtgtag cgttcggtaa cctgttgcag ggcgtaccgt tcaacgttga
     4741 tgaatatctg cgtctgtact acaccggtaa cttcttccag ttgcttaacc cgttcggcct
     4801 gctggcaggc gtggtgagcg tagggatgat cattactcag ggcgcaacct atctgcaaat
     4861 gcgtaccgtg ggcgaactgc acctgcgtac ccgtgcaacg gctcaggtgg ctgcgctggt
     4921 gacactggtc tgtttcgcac tggctggcgt atgggtgatg tacggtatcg atggttatgt
     4981 cgtgaaatcg acaatggacc attacgcagc ctctaaccca ctgaataaag aagtggttcg
     5041 tgaagctggc gcatggctgg ttaacttcaa caacacgcca attctgtggg ctattccggc
     5101 actgggtgtg gttctgccgc tgctgaccat cctgactgca cgtatggata aagccgcgtg
     5161 ggcgtttgtg ttctcctccc tgacgctggc ctgcatcatc ctgacagccg gtatcgcaat
     5221 gttcccgttt gtgatgccgt ccagcaccat gatgaacgca agtctgacaa tgtgggatgc
     5281 aacttccagc cagctgacgc ttaacgtcat gacctgggtt gcggtggttc tggtaccgat
     5341 cattctgctc tacaccgcct ggtgttactg gaaaatgttc ggtcgtatca ccaaagaaga
     5401 tattgaacgt aacacccact ctctgtacta agtaaggagc taaaaatgtg gtatttcgca
     5461 tggattctgg gaacgcttct tgcctgttcg tttggggtaa tcaccgcgct ggcgcttgaa
     5521 cacgtcgaat caggcaaagc cggtcaagaa gacatctgat gagtaagatt atcgcgactt
     5581 tgtatgcggt aatggacaag cgccccctgc gggcgctttc cttcgtgatg gcgcttctgt
     5641 tagcaggatg tatgttttgg gacccatctc gttttgccgc gaagaccagt gaactggaaa
     5701 tctggcatgg tttattgctg atgtgggccg tctgtgctgg tgtgattcac ggcgtgggct
     5761 ttcgtccgca gaaggttctt tggcaaggga ttttttgccc attgcttgcc gatattgttc
     5821 tcattgtcgg gctgattttc ttcttctttt aaatcagaat tctctttaaa aaattatggg
     5881 ccgctccagg cccataaatt tttacgctcc cttaacttgc cctcattccc aaacctcaat
     5941 cgcgcgcgta tagtagcagc gtttaaaagc tctaactttt gttgcattac cgggatgtaa
     6001 agtgaataca acgctgtttc gatggccggt tcgcgtctac tatgaagata ccgatgccgg
     6061 tggtgtggtg taccacgcca gttacgtcgc tttttatgaa agagcacgca cagagatgct
     6121 gcgtcatcat cacttcagtc agcaggcgct gatggctgaa cgcgttgcct ttgtggtacg
     6181 taaaatgacg gtggaatatt acgcacctgc gcggctcgac gatatgctcg aaatacagac
     6241 tgaaataaca tcaatgcgtg gcacctcttt ggttttcacg caacgtattg tcaacgccga
     6301 gaatactttg ctgaatgaag cagaggttct ggttgtttgc gttgacccac tcaaaatgaa
     6361 gcctcgtgcg cttcccaagt ctattgtcgc ggagtttaag cagtgactga catgaatatc
     6421 cttgatttgt tcctgaaggc tagccttctg gttaaactta tcatgttgat tttgattggt
     6481 ttttcaatcg catcttgggc cattattatc cagcggaccc gtattcttaa cgcagcggcg
     6541 cgcgaagccg aagcgtttga agataaattc tggtctggaa tcgaactctc tcgcctctat
     6601 caagagagcc aggggaaacg ggataatctg actggttcgg aacaaatctt ttacagcggg
     6661 ttcaaagagt ttgtgcgcct gcatcgtgcc aatagccatg cgccggaagc cgtagtggaa
     6721 ggggcgtcgc gtgctatgcg tatctccatg aaccgtgaac ttgaaaatct ggaaacgcac
     6781 attccgttcc tcggtacggt tggctccatc agcccgtata ttggtctgtt tggtacggtc
     6841 tgggggatca tgcacgcctt tatcgccctc ggggcggtaa aacaagcaac actgcaaatg
     6901 gttgcgcccg gtatcgcaga agcgttgatt gcgactgcaa ttggtctgtt cgccgcaatt
     6961 ccggcggtaa tggcttataa ccgcctcaac cagcgcgtaa acaaactgga actgaattac
     7021 gacaacttta tggaagagtt taccgcgatt ctgcaccgcc aggcgtttac cgttagcgag
     7081 agcaacaagg ggtaagccat ggccagagcg cgtggacgag gtcgtcgcga tctcaagtcc
     7141 gaaatcaaca ttgtaccgtt gctggacgta ctgctggtgc tgttgctgat ctttatggcg
     7201 acagcgccca tcatcaccca gagcgtggag gtcgatctgc cagacgctac tgaatcacag
     7261 gcggtgagca gtaacgataa tccgccagtg attgttgaag tgtctggtat tggtcagtac
     7321 accgtggtgg ttgagaaaga tcgcctggag cgtttaccac cagagcaggt ggtggcggaa
     7381 gtgtccagcc gtttcaaggc caacccgaaa acggtctttc tgatcggtgg cgcaaaagat
     7441 gtgccttacg atgaaataat taaagcactg aacttgttac atagtgcggg tgtgaaatcg
     7501 gttggtttaa tgacgcagcc tatctaaaca tctgcgtttc ccttgcttga aagagagcgg
     7561 gtaacaggcg aacagttttt ggaaaccgag agtgtcaaag gcaaccgaac aaaacgacaa
     7621 gctcaagcgg gcgataatta tttcagcagt gctgcatgtc atcttatttg cggcgctgat
     7681 ctggagttcg ttcgatgaga atatagaagc ttcagccgga ggcggcggtg gttcgtccat
     7741 cgacgctgtc atggttgatt caggtgcggt agttgagcag tacaaacgca tgcaaagcca
     7801 ggaatcaagc gcgaagcgtt ctgatgaaca gcgcaagatg aaggaacagc aggctgctga
     7861 agaactgcgt gagaaacaag cggctgaaca ggaacgcctg aagcaacttg agaaagagcg
     7921 gttagcggct caggagcaga aaaagcaggc tgaagaagcc gcaaaacagg ccgagttaaa
     7981 gcagaagcaa gctgaagagg cggcagcgaa agcggcggca gatgctaaag cgaaggccga
     8041 agcagatgct aaagctgcgg aagaagcagc gaagaaagcg gctgcagacg caaagaaaaa
     8101 agcagaagca gaagccgcca aagccgcagc cgaagcgcag aaaaaagccg aggcagccgc
     8161 tgcggcactg aagaagaaag cggaagcggc agaagcagct gcagctgaag caagaaagaa
     8221 agcggcaact gaagctgctg aaaaagccaa agcagaagct gagaagaaag cggctgctga
     8281 aaaggctgca gctgataaga aagcggcagc agagaaagct gcagccgaca aaaaagcagc
     8341 agaaaaagcg gctgctgaaa aggcagcagc tgataagaaa gcagcggcag aaaaagccgc
     8401 cgcagacaaa aaagcggcag cggcaaaagc tgcagctgaa aaagccgctg cagcaaaagc
     8461 ggccgcagag gcagatgata ttttcggtga gctaagctct ggtaagaatg caccgaaaac
     8521 ggggggaggg gcgaaaggga acaatgcttc gcctgccggg agtggtaata ctaaaaacaa
     8581 tggcgcatca ggggccgata tcaataacta tgccgggcag attaaatctg ctatcgaaag
     8641 taagttctat gacgcatcgt cctatgcagg caaaacctgt acgctgcgca taaaactggc
     8701 acccgatggt atgttactgg atatcaaacc tgaaggtggc gatcccgcac tttgtcaggc
     8761 tgcgttggca gcagctaaac ttgcgaagat cccgaaacca ccaagccagg cagtatatga
     8821 agtgttcaaa aacgcgccat tggacttcaa accgtaatcg cgatgttgac tgttcggacg
     8881 gtcaacatca ggcaccggtt gccacggggt tctggtagtt ttgtgtattt tagtttgtta
     8941 acattctgct aaattatcgt gggccatcgg tccagataag ggagatatga tgaagcaggc
     9001 attacgagta gcatttggtt ttctcatact gtgggcatca gttctgcatg ctgaagtccg
     9061 cattgtgatc gacagcggtg tagattccgg tcgtcctatt ggtgttgttc ctttccagtg
     9121 ggcggggcct ggtgcggcac ctgaagatat tggcggcatc gttgctgctg acttgcgtaa
     9181 cagcggtaaa tttaatccgt tagatcgcgc tcgtctgcca cagcagccgg gtagtgcgca
     9241 ggaagtacaa ccagctgcat ggtccgcact gggcattgac gctgtagttg tcggtcaggt
     9301 cactccgaat ccggatggtt cttacaatgt tgcttatcaa cttgttgaca ctggcggcgc
     9361 accgggtact gtacttgctc agaactcgta caaagtgaac aagcagtggc tgcgttatgc
     9421 tggtcatacc gccagtgatg aagtgtttga aaaactgacc ggcattaaag gtgcgttccg
     9481 tactcgtatt gcctacgttg ttcagaccaa cggcggtcag ttcccgtatg aactgcgcgt
     9541 atctgactat gacggttaca accagtttgt cgttcaccgt tcaccgcagc cgctgatgtc
     9601 tccggcgtgg tcaccagacg gttctaaact ggcttatgtg accttcgaaa gcggtcgttc
     9661 cgcgctggtt attcagacgc tggcaaatgg cgctgtacgt caggtggctt cattcccgcg
     9721 tcacaacggt gcacctgcat tctcgccaga cggcagcaaa ctggcattcg ccttgtcgaa
     9781 aaccggtagt ctgaacctgt acgtaatgga tttggcttct ggtcagatcc gccaggtgac
     9841 tgatggtcgc agtaacaata ccgaaccgac ctggttcccg gatagccaga acctggcatt
     9901 tacttctgac caggccggtc gtccgcaggt ttataaagtg aatatcaacg gcggtgcgcc
     9961 acaacgtatt acctgggaag gttcgcagaa ccaggatgcg gatgtcagca gcgacggtaa
    10021 atttatggta atggtcagct ccaatggtgg gcagcagcac attgccaaac aagatctggc
    10081 aacgggaggc gtacaagttc tgtcgtccac gttcctggat gaaacgccaa gtctggcacc
    10141 taacggcact atggtaatct acagctcttc tcaggggatg ggatccgtgc tgaatttggt
    10201 ttctacagat gggcgtttca aagcgcgtct tccggcaact gatggacagg tcaaattccc
    10261 tgcctggtcg ccgtatctgt gataataatt aattgaatag taaaggaatc attgaaatgc
    10321 aactgaacaa agtgctgaaa gggctgatga ttgctctgcc tgttatggca attgcggcat
    10381 gttcttccaa caagaacgcc agcaatgacg gcagcgaagg catgctgggt gccggcactg
    10441 gtatggatgc gaacggcggc aacggcaaca tgtcttccga agagcaggct cgtctgcaaa
    10501 tgcaacagct gcagcagaac aacatcgttt acttcgatct ggacaagtac gatatccgtt
    10561 ctgacttcgc tcaaatgctg gatgcacatg caaacttcct gcgtagcaac ccgtcttaca
    10621 aagtcaccgt agaaggtcac gcggacgaac gtggtactcc ggaatacaac atctccctgg
    10681 gtgaacgtcg tgcgaacgcc gttaagatgt acctgcaggg taaaggcgtt tctgcagacc
    10741 agatctccat cgtttcttac ggtaaagaaa aacctgcagt actgggtcat gacgaagcgg
    10801 catactccaa aaaccgtcgt gcggtactgg tttactaaga gaattgcatg agcagtaact
    10861 tcagacatca actattgagt ctgtcgttac tggttggtat agcggccccc tgggccgctt
    10921 ttgctcaggc accaatcagt agtgtcggct caggctcggt cgaagaccgc gtcactcaac
    10981 ttgagcgtat ttctaatgct cacagccagc ttttaaccca actccagcaa caactttctg
    11041 ataatcaatc cgatattgat tccctgcgtg gtcagattca ggaaaatcag tatcaactga
    11101 atcaggtcgt ggagcggcaa aagcagatcc tgttgcagat cgacagcctc agcagcggtg
    11161 gtgcagcggc gcaatcaacc agcagcgatc aaagcggtgc ggcggcatca acgacgccga
    11221 cagctgatgc tggtactgcg aatgctggcg cgccggtgaa aagcggcgat gcaaacacgg
    11281 attacaatgc agctattgcg ctggtgcagg ataaatcccg ccaggatgac gcaatggtgg
    11341 catttcagaa tttcatcaaa aattaccctg attcaactta cctgccaaac gccaattatt
    11401 ggctgggtca gttaaactac aacaagggta aaaaagatga tgcggcgtac tattttgctt
    11461 cggtagtgaa aaactatccg aagtcaccaa aggctgctga tgcgatgttt aaagtcggcg
    11521 tcatcatgca ggacaaaggt gacaccgcaa aagcgaaagc cgtataccag caggttatca
    11581 gtaaataccc tggtactgat ggcgctaaac aggcacaaaa acgtctgaac gcgatgtaat
    11641 gcataacaca cgaccagaag tcgcattatt tctggtcgtg tcgtgcgaat cataagcagt
    11701 tgagtgatct gcatcgaaat ttttgttgcg ctcaagtctg aaatcagtaa tatatgccgc
    11761 cgttgccacg ggatatcaaa caaaccgaaa gcaacgaaaa agtgggtcgt tagctcagtt
    11821 ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccaa
    11881 tcgctaaggt ggaagcggta gtaaaacgtg aaggataacg ttgcatcagc aacggcccga
    11941 agggcgagac gaagtcgagt catcctgcac gacccaccac taacatagtt agttgtagta
    12001 tccagcgtag tattgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg
    12061 tcggcggttc gatcccgtca tcacccacca ccgggtcgtt agctcagttg gtagagcagt
    12121 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccagt tttagcatcg
    12181 aagacaagat gttaagcgtg taggataacg ttgcgtcagc aacggcccgt agggcgagcg
    12241 aagcgagtca tcctgcacga cccaccacta attacggtgg gttcggtgga agtagtttgt
    12301 agtatccagc gcagtat
//
LOCUS       DAFATA010000121        12286 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFATA010000121 DAFATA010000000
VERSION     DAFATA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.122"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..153)
                     /locus_tag="LGJ20_004092"
     CDS             complement(<1..153)
                     /locus_tag="LGJ20_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBK2388095.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMT"
     regulatory      complement(257..405)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            527..817
                     /gene="ubiK"
                     /locus_tag="LGJ20_004093"
     CDS             527..817
                     /gene="ubiK"
                     /locus_tag="LGJ20_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBK2388096.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            1100..1651
                     /locus_tag="LGJ20_004094"
     CDS             1100..1651
                     /locus_tag="LGJ20_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBK2388097.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            1711..4215
                     /locus_tag="LGJ20_004095"
     CDS             1711..4215
                     /locus_tag="LGJ20_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2388098.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            4231..4980
                     /locus_tag="LGJ20_004096"
     CDS             4231..4980
                     /locus_tag="LGJ20_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2388099.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            4982..6046
                     /locus_tag="LGJ20_004097"
     CDS             4982..6046
                     /locus_tag="LGJ20_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388100.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEINRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(6089..6289)
                     /gene="glgS"
                     /locus_tag="LGJ20_004098"
     CDS             complement(6089..6289)
                     /gene="glgS"
                     /locus_tag="LGJ20_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBK2388101.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            6558..7187
                     /locus_tag="LGJ20_004099"
     CDS             6558..7187
                     /locus_tag="LGJ20_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBK2388102.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7214..8875
                     /locus_tag="LGJ20_004100"
     CDS             7214..8875
                     /locus_tag="LGJ20_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK2388103.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            9114..9173
                     /locus_tag="LGJ20_004101"
     CDS             9114..9173
                     /locus_tag="LGJ20_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBK2388104.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            9490..9549
                     /gene="ibsE"
                     /locus_tag="LGJ20_004102"
     CDS             9490..9549
                     /gene="ibsE"
                     /locus_tag="LGJ20_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBK2388105.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(9669..11102)
                     /gene="hldE"
                     /locus_tag="LGJ20_004103"
     CDS             complement(9669..11102)
                     /gene="hldE"
                     /locus_tag="LGJ20_004103"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBK2388106.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11150..>12286)
                     /gene="glnE"
                     /locus_tag="LGJ20_004104"
     CDS             complement(11150..>12286)
                     /gene="glnE"
                     /locus_tag="LGJ20_004104"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBK2388107.1"
                     /translation="LARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQ
                     QLEALRQFKQAQLLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYG
                     KPNHLNDREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFY
                     LRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQA
                     LVRARVVYGDPQLTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFD
                     IKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTR
                     AYTTLRDELHHLALQELPGHVPEDCFTAERELVRASWQKWLVEE"
BASE COUNT         3456 a         2909 c         2738 g         3183 t
ORIGIN      
        1 agtcatggtt tctgccggga agatcatatc accttcgttt tcacggtctt catcatcaag
       61 caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac gttcgaaagg
      121 cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaacctcact aaaattatgg
      181 ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac gtgagcgggt
      241 ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg ccccggaatt
      301 acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt tcaactgagt
      361 tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt cactataacg
      421 ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga cttaccctta
      481 tcgcactaca atggcactca acacctatca gtacagggaa actacaatga ttgacccgaa
      541 aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa tcagggagtt
      601 cggggaagat gtggagaaaa aaatccgcca aaccctacaa gctcagctga cgcgcctcga
      661 ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga ctcgtgagaa
      721 actggcgctg cttgaacaaa gaatgagcga actggaaaat cgcagtactg agattaaaaa
      781 gcagccagat ccagagactc tccccccaac gctgtaaaca tttgtcttta atgcactgta
      841 aattatgaat taatcttcta atttacagtg ctttctatta ccttatcgct taattccatt
      901 cagaatatca tcacaaaaat gaatgagtgc aattgaaaat ataattgtta tgtatttttt
      961 aagttttact taatccatat ttagataaac taatttcata aaacccgaaa agtaaaatat
     1021 aatatttgaa tgattagcaa tccttaaata acagttacta atcattcatt ttctacttat
     1081 atttatataa aggaatatta tgtctgcttt taaaaagtca cttcttgttg ctggcgtagc
     1141 aatgattctt tccaataacg tatttgctga cgaaggtcac ggcattgtta aatttaaagg
     1201 tgaagttatc tctgctccat gttcaattaa accaggtgac gaagacttaa ctgtaaatct
     1261 gggtgaagta gcagataccg ttctgaaaag cgaccagaaa tctttggctg agcctttcac
     1321 tattcacctg caagactgca tgttgagcca gggtggaacc acttattcaa aagcgaaggt
     1381 tactttcacc acagcgaata ctatgacggg tcagactgac ctgctgaaaa acaccaaaga
     1441 aactgaaatc ggtggtgcca caggcgttgg cgtacgtatt ctggatagcc agagcggcga
     1501 agtgacttta ggtactccgg tagtaattac attcaacaat actaacagct atcaggaact
     1561 gaacttcaaa gctcgtatgg aatccccaag caaagatgct accccgggca acgtttacgc
     1621 tcaggctgac tataagattg cttacgagta atcacattag gccgggttcg cccggccaca
     1681 tcattaacgg attaatgata agtggaacag atgtataaaa aattaaaatt aaccacaata
     1741 agcgaattga ttaaaaatat ttattgttca ttatccgttc tcatcattgg ttgtgcgtca
     1801 gcttatgccg ttgaattcaa caaagattta atcgaagccg aagatcgtga aaacgttaac
     1861 ctttcccaat ttgaaactga tggccaatta cccgtaggta aatactcatt aaacgctctt
     1921 attaataata aaaggacgcc aatccatctg gacctgcaat gggtattaat tgataaccaa
     1981 actgcagttt gtctgactcc agagcaatta acattattag gctttactga tgagattatt
     2041 gaagtggctc agcaaaacct gatcgatggt tgttacccta tcgaaaaaga aaaacaaatt
     2101 acaacttatc tcgataaagg gaaaatgcaa ttatccatat ctgcacctca ggcatggtta
     2161 aaatacaaag atgcaaactg gacgcctcct gaactttggg atcatggtat tgctggggca
     2221 tttcttgact acaatttata tgcctctcat tatgcaccac atcagggcga taattcacaa
     2281 aatataagtt cctatgggca ggctggggtt aatcttgggg cctggcgcct gcgtactgat
     2341 taccagtacg atcaatcatt taacaatggc aaaagccagg cgaacaacct ggattttccg
     2401 cgtatttatt tgtttcgccc aatcccagca attaatgcaa aactaactat aggtcaatac
     2461 gatactgaat cctctatttt cgactctttc catttttctg gcgtttcgtt gaaaagcgat
     2521 gagaatatgt tgccgcctga tctacgtggt tacgcaccgc aaatcacggg tgttgcacaa
     2581 acgaatgcaa aggtcactgt ctcacagaac aaccgtatta tttatcaaga aaatgttcct
     2641 cctggcccat ttgctattac taatttattc aatacattac aggggcaact ggacgtcaag
     2701 gttgaagaag aggacgggca agtcacgcaa tggcaagttg catctaatag tattccttat
     2761 ctgacgcgta aagggcagat tcgctacacc actgctatgg gtaaaccgac cagcgttggt
     2821 ggtgattcct tacaacaacc cttcttctgg actggtgaat tctcatgggg ttggctgaac
     2881 aatgtatccc tgtatggtgg ttcagtttta acaaaccgtg attatcaatc tctggctgcc
     2941 ggcgttggct ttaaccttaa ctcattgggt tcattatctt ttgatgtcac acgatctgat
     3001 gctcagttgc ataatcagga taaagaaacg ggttatagct accgcgctaa ctattcaaaa
     3061 cgttttgaat ctaccggtag ccagctcact ttcgctggtt accgtttctc tgataaaaac
     3121 tttgtgacaa tgaatgaata tatcaatgac actaaccatt acacgaatta tcagaatgaa
     3181 aaagagagtt atattgtcac gtttaaccag tatcttgaat cattaaggtt aaatacatac
     3241 gtaagtttgg ctcgtaatac ttactgggac gccagcagta atgtgaatta ttcattatca
     3301 cttagccgcg attttgatat cgggccatta aaaaacgtct ccacttctct aacatttagc
     3361 cgaataaact gggaagaaga caaccaggat caactgtacc taaatatttc gattccctgg
     3421 ggaaccagta gaacattgag ctatggtatg caacgaaatc aggataacaa gatttcgcat
     3481 actgcttcgt ggtatgactc ttccgatcga aataattcct ggagcgtttc tgcttcaggc
     3541 gacaatgatg aattcaaaga tatgaaagcg tcactacgcg ccagttatca gcataatacc
     3601 gagaacggtc gtctctatct ctccgggaca tcacagcgag acagttatta ttctctgaat
     3661 gccagttgga atggttcatt cacggcgact cgtcacggag ccgctttcca tgactatagt
     3721 ggtagtgctg actcgcgttt tatgatcgac gcagatggtg cagaagatat tccgttgaac
     3781 aataaacgcg cggtaactaa tcggtatggc atcggagtta ttccatcagt cagcagttac
     3841 ataacaacat cattaagcgt tgatacccga aatcttccag aaaatgtgga tatcgaaaac
     3901 tcggttatca ccaccacctt aaccgaaggt gctattggct acgccaaact tgatacccgc
     3961 aagggctatc aaatcatggg ggttattcgc ctggcagatg gtagtcaccc accactgggg
     4021 attagcgtaa aagataaaac cagccataaa gaattaggac tggttgctga tggcggcttt
     4081 gtatacctca acggcattca ggatgacagc aaacttactt tacgctgggg tgataaatcc
     4141 tgttttattc aaccacccaa tagcagcaac ttaaccaccg gaacggttat tttaccgtgt
     4201 attagccaaa attaattcag gatgaaacta atgcgctact taaacacaaa gaatctcatt
     4261 gcagcaggag tattactcgc ctgcatgagc agcattgcct ggggtgccat catcccggac
     4321 cgtacccgta tcataatgaa tgagtcagat aaaggggaag cattaaaact tactaatcaa
     4381 agcaaaaatc taccctatct ggcgcaaaca tggatagaag atacgaaagg caataaatca
     4441 cgtgacttta ttgtcacggt tccccctatg gtacgtttaa atcccagtga gcaaattcaa
     4501 ataagaatga taagtcagga aaaaattgct caactgccta atgacagaga aacgctcttc
     4561 tattttaacg tgcgagaaat accgccaaaa acagataaaa aaaatgtgat gcaagtcact
     4621 atgcagcacg cattgaaact attctggcga ccaaaagcta ttgaattaga agatgatggg
     4681 gtaatgtctt acgaaaaagt tgaaatcatc cgtagaaatg atggttcgat ccgctttaac
     4741 aataagatgc cttatcacgt tacgctgggt tatatcggaa caaacggaat cacaatgttg
     4801 ccacaaacgc aaagtttaat ggtaacccct tatagccatg cagatacgca gtttaaaaat
     4861 gttccgtcag cttttcaggt tggttatatc aatgattttg gtggattaag tttttatgaa
     4921 ataaactgcc cgacggttaa taactcttgt aatgtttccg tagccaaacg agataaataa
     4981 tatgcgctac ttattaattg taattatatt tattataggt tttagtgcat tacctgtatg
     5041 ggcaatggac tgctatgctg aacatgaagg gggaaatacc gtagtcattg gttacgtacc
     5101 aagaatttct atccccagta atggtaaaaa aggtgataag atctggcaaa gcagtgagta
     5161 ttttatgaat gtcttctgta ataatgcatt acctgcccca tcacctggag aagaataccc
     5221 atcagcctgg actaatataa tgatgttttt accaggcggt caggactttt ataatcagaa
     5281 ctcctatatt tttggcgtaa catacaatgg agttgattat gattcgactg cccctaatgc
     5341 tatcgcagca ccagaatgca ttgatataaa aggagcgggt acctttaata atcactataa
     5401 aaaccccgct gtctgtagtg gaggtcctga gcctcaatta tcagtaacct ttccagctcg
     5461 cgtacaactc tatattaaat tagcaaaaaa tgccaataga gttaataaaa acctggtgtt
     5521 acctgatgaa tatatcgctc tagagtttaa aggtatgagt ggcgcaggag ctatagaagt
     5581 agacaaaaat ttgaccttca ggattcgtgg attaaataat attcatgtcc ttgactgctt
     5641 tgttaatgtc gcactggaac cagctgatgg cgttgttgac tttggtaaaa taaattcccg
     5701 aacaattaaa aataccagcg ttagtgagac gtttagtgta gtcatgacaa aagatcctgg
     5761 tgcggcctgt actgagcaat tcaatatttt agggagcttt ttcactacgg atattttgag
     5821 tgattatagc catctggata tgggtaatgg actgctattg aagatatttc ataaagatgg
     5881 aacagcaacg gaaattaacc gcttctcaca atttgcttct ttttcatcgt ctagtgcacc
     5941 ttcggtcacc gcaccattca gggcagaact tagtgcgaat ccggcagaga ctgttgttga
     6001 gggaccgttt agtaaagacg tcatcctcaa aatcacctat aactagcatc taatacaaac
     6061 actaaaacgg gccatcaggc ccgttatatc agtgctctaa ctccagctct cttgcctgca
     6121 tcatctgttg acaataccag gcataacgtg cgcaaaacca ggttctatgt tcttcatcca
     6181 tgttaccagt aacggcaaga atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac
     6241 tacgcgccag gaaatcgaaa ttatttaaag aattaagact atgatccata agcacatcct
     6301 ccggttatcg catacctttt aaggccatat gctttttctg atttcagtat tccccaatca
     6361 gtgtagtgcc tttaaacata acgtgcgtaa atatattgtc cccgatcaca ctttttagtg
     6421 aaaaataaaa aaccatatta aaaaactttc tcttccagaa cttctctaac atgtgttatt
     6481 attaacaaag ataaatgtaa gcaaggaaac tatcccaaag taaatattgc gtcactaaat
     6541 ggacattgga gtgacatatg attttattcg ccgactataa taccccttat cttttcgcta
     6601 tttcctttgt actgttgatc ggccttttag aaattttagc gcttatctgc ggtcacatgc
     6661 tctccggtgc tcttgatgca catcttgacc attatgattc cataaccaca ggtcatatta
     6721 gccaggcact tcattatctc aatatcggaa gattgccagc tctcgtcgtt ctctgcttgc
     6781 tggctggttt ttttggtctt attggcatcc tgttgcagca tgcctgcatc atggtctggc
     6841 aatcaccact gtcgaacctg ttcgtggttc ctgtcagttt actgtttacg attattgcgg
     6901 tgcattatac cggtaaaata gttgcgccct ggattccacg cgaccatagt tcagctatta
     6961 cagaagaaga atatattggt agcatggcat taattaccgg tcatcaagcc acttcaggta
     7021 atccctgcga aggaaaactc accgatcaat ttggccaaat acattattta ctactggagc
     7081 ctgaagaagg aaaatttttc actaaaggag acaaagtgtt aattatttgc cgactttctg
     7141 caacgcgata tttggcggaa aacaatccct ggccacaaat attgtaattt attaatcaaa
     7201 aggaaattta ataatggatg atattgttaa ttctgtgccc tcctggatgt ttaccgcgat
     7261 tattgccgta tgcattctgt ttattattgg aattattttc gccaggctct atcgtcgcgc
     7321 ttcggcagag caagcttttg ttcgtactgg tttaggtggg caaaaagtgg tgatgagcgg
     7381 tggcgcaatc gtgatgccga tctttcatga aataatcccc atcaatatga atactctgaa
     7441 gctggaagtc agccgctcaa ccattgatag cctgattacg aaagatcgta tgcgtgttga
     7501 tgtagtagtc gctttctttg tacgggtaaa accgtctgta gaaggcatcg ccactgcggc
     7561 gcagacgttg gggcaacgca cgctatcacc agaagactta cgtatgttgg ttgaagataa
     7621 atttgtcgat gccctccgtg caacagctgc acaaatgacc atgcatgagt tacaggatac
     7681 ccgcgagaac ttcgtgcagg gagtacaaaa tactgtcgca gaagacctgt cgaaaaacgg
     7741 tcttgagctg gaaagcgttt cacttaccaa ctttaaccag acctcgaaag aacatttcaa
     7801 tccgaacaat gcctttgacg ccgaaggttt aaccaaactg actcaggaga cggagcgccg
     7861 tcgtcgcgaa cgtaacgaag ttgaacagga tgtagaagtt gcggtgcgtg agaaaaaccg
     7921 tgatgcactt tcgcgcaagt tggagattga acaacaagaa gcgtttatga cgcttgagca
     7981 ggagcagcag gttaaaaccc gtactgccga acagaatgca cgtattgcgg cttttgaagc
     8041 tgaacgtcgt cgtgaagcag agcagacgcg aattctggct gaacgacaaa ttcaggaaac
     8101 ggagatcgag cgcgagcagg ccgtccgctc aagaaaggtt gaggctgaac gtgaagttcg
     8161 tattaaagag atcgaacaac agcaggtcac cgaaatcgcc aaccagacga aatcgatcgc
     8221 tattgccgcc aaatcggaac aacagtccca ggcagaagcg cgtgctaacc ttgcactcgc
     8281 agaagcagta agtgcgcaac aaaacgtaga aaccactcgc cagaccgcag aagccgatcg
     8341 tgctaaacaa gttgccctaa tcgctgccgc gcaggatgca gaaaccaaag cggttgaact
     8401 gaccgtgcgg gcgaaagcag aaaaagaagc cgcagagatg caggcggcgg ctatcgttga
     8461 gttagccgaa gctacacgta aaaagggtct ggcggaagca gaagcacaac gtgcgctgaa
     8521 cgatgctatc aacgtacttt ctgatgaaca aaccagcctt aaattcaaac tggccttgtt
     8581 gcaggcgcta cctgcggtaa tagaaaaatc cgttgagccg atgaaatcta ttgacggtat
     8641 caagattatt caggtcgatg gtctgaatcg tggcagcgct gcgggtgatg caaacacggg
     8701 taatgtgggg ggcggaaacc tggcggaaca agcattatca gccgctctct cttaccgcac
     8761 acaggcaccg ctgattgact ccttgctcaa tgaaattggc gtttcaggcg gctcactggc
     8821 ggcattgact tcacccttaa cctcaacaac tcccgtcgcc gaaaacgtag aataaatttt
     8881 cagctatgaa cgtccctggc acaaaaagcc ggggattttt tatatctgcg ttccgctaaa
     8941 aactgcaaat gatcaggtcg ttgcaacgtt ttgcgtgacc gctcggggaa ggcaaaactg
     9001 cctctgggaa agcgttgcgc gaggtccggc gctcatcaac aatcggggag tcagcaaagg
     9061 gctgaaacgg gaaagcccct cccgaggaag gggccttaca gaaggaaagg gttatgatga
     9121 agctcgtcat catactgatt gtgttgttac tcgtaagttt cgcagcttat taacagccaa
     9181 tcagaggggg gagaaatcct ccctcaccct tgttccttta ctttaggttg aaaaaaaatc
     9241 agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg attttttata
     9301 tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc gtgaccgctc
     9361 ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc atcaacaatc
     9421 ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc cttatagaag
     9481 gaaagggtta tgatgaagtt cgtcatcata ctggttgtgt tgttactgtt aagtttcccg
     9541 acttactaac aactgatcag aggggggaga aatcctccct tacccttgtt cctttacttt
     9601 aggttgaaaa aacaacagcg tcaataggcc tgcctgctac gaagcgagat ctgtgaaccg
     9661 ctttccggtt agcctttttt atcctgttgg atcttcttga tgatgttagt ggtcgagcaa
     9721 ccgtcttcga agttgagcac cagcacttcg ccgccgttgg cccagacttc tttactcccg
     9781 gcaatttctt ctggtttata gtcgccgcct ttcaccagca gatctggcag gatcccggcg
     9841 atcagacgct gcggcgtgtc ttcttcaaac gacactaccc agtcgaccgc ttccagtgcg
     9901 cccagcacaa tcatacgctg ctcgagtggg tttaccgggc gggaatcccc tttcagccgt
     9961 ttggtggagg catcgctgtt cacagcgaca atcagacggt cacccagttt gcgagcattt
    10021 gccagatagg agacgtgccc ggcgtgcagg atgtcaaaga caccgttggt cattaccacc
    10081 ttttcgccgc gtttacgcgc tgccgctaca gcaagcttca gttcctcttc ggtcatcaca
    10141 ccaaagccgg tatctgcgcg cccacgcact gcattttcca gctcgatcgg cgaaaccgtg
    10201 gaggttccca gtttgccgac caccacgcca gccgccgcat tggcaaagaa gcaggcttct
    10261 tccagcgaat tacccgctgc cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca
    10321 ccggtaacgt catacacttc ctgcgcctgg gttggcatat gcagcggcgc tttacccggt
    10381 tgcagcagcg acataccctg ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg
    10441 gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt accgacaaca
    10501 gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg
    10561 gtaccttttg gatcaatcag caccggaacg cccgctttac gcgccagttg gatcatctgc
    10621 tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca ccagcgcgcc aatcgaactc
    10681 agcgcctgat taatccgctc gtgcagcggc tgcggatcaa caccttcgaa accttcttca
    10741 aaatccaggc ggatcagctg ttggttgcgg gaaagtaccc gtaatttggt gatagtcgga
    10801 tgcgtcggta cagaaacgaa gtcgcatttg acgttgacgt cggccagaga tttactcagc
    10861 gcacgcgccg catcgtcaat gcccgtcagc ccgaccaggc gagcattggc accgagagaa
    10921 gcgatgttca ttgccacgtt agccgcgccg cccggacgtt cttcgatggt attgacttta
    10981 accacgggca ccggcgcttc cggcgagata cggctggtgg gaccgtacca gtaacgatcc
    11041 agcatcacat caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc
    11101 attcctgtct cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca
    11161 ccagccactt ctgccagctt gcccgcacca gttcacgctc tgcggtgaag caatcctccg
    11221 gcacatggcc cggcaattcc tgtaatgcca gatgatgaag ttcatcgcgc agcgtagtat
    11281 aagcacgggt cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagtagtt
    11341 ccagaatgcg cacgttgtct gaccagcgcg ttaacttcgg tttttcatga gcgtagcgca
    11401 acaccagata ttgggtaata aattcgatat cggtaattcc gccttcatca gctttgatat
    11461 caaagcgatc gcgatgttta ttgccgagat gagcgcgcat tttctcgcgc atttcccgca
    11521 cttccgtttg cagagtttta ccttcacgcg gcagcgtcat aatctcgcga cgcaccgcgt
    11581 caaagtgcgc ggtgagctgc ggatcgccgt acactacccg cgcacgcacc agcgcctgat
    11641 gttcccacgt ccaggcctcg tttttctgat aatcggcaaa tgcttctgcg gatgtcacca
    11701 gcattcccgc ggccccggac gggcgtagcc gtgcatccac ttcatacaaa atgccggaag
    11761 aggtacgcgt actgaacaga tgcataatgc gttgcgccag acgcagataa aactgtcgcc
    11821 cgtcgatttc ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagatca
    11881 ggtcaagatc ggaactgtag cctaactccc agccgcccag cttgccgtag ccgaccaccg
    11941 caaaaccacg gccttcgcga tcgttgaggt gatttggctt accgtagcgg gccaccattt
    12001 gaacccacgc ctgctgaacg acggcatcta tcatagcctc tgccagccag gttaagtgat
    12061 cgctcacttt catcaccggt agcgtaccag cgatatccgc tgcggcgatg cgtaacagct
    12121 gcgcctgttt gaactgacgc agcgcctcaa gctgttgctc ttcgtcatct tccggcacgc
    12181 gcagcaaata ctggcgcaac tcatcgcggt aggcatcggt cgccgtcggc tggtaaaggg
    12241 tgtttggatc gagcaattca tccagcaata atggataacg cgccag
//
LOCUS       DAFATA010000122        12247 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFATA010000122 DAFATA010000000
VERSION     DAFATA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.123"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..2141
                     /gene="rpoB"
                     /locus_tag="LGJ20_004105"
     CDS             <1..2141
                     /gene="rpoB"
                     /locus_tag="LGJ20_004105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK2388108.1"
                     /translation="EDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHD
                     DANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRI
                     VIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTD
                     LGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPE
                     EITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIF
                     GEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEEL
                     QILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELK
                     HEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKIN
                     PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEV
                     AKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIK
                     ELLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLV
                     TQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGN
                     HQMEPGMPESFNVLLKEIRSLGINIELEDE"
     gene            2218..6441
                     /gene="rpoC"
                     /locus_tag="LGJ20_004106"
     CDS             2218..6441
                     /gene="rpoC"
                     /locus_tag="LGJ20_004106"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK2388109.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            6654..7193
                     /locus_tag="LGJ20_004107"
     CDS             6654..7193
                     /locus_tag="LGJ20_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HBK2388110.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(7603..8736)
                     /gene="thiH"
                     /locus_tag="LGJ20_004108"
     CDS             complement(7603..8736)
                     /gene="thiH"
                     /locus_tag="LGJ20_004108"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBK2388111.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALIAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(8733..9503)
                     /gene="thiG"
                     /locus_tag="LGJ20_004109"
     CDS             complement(8733..9503)
                     /gene="thiG"
                     /locus_tag="LGJ20_004109"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBK2388112.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVLVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(9505..9705)
                     /gene="thiS"
                     /locus_tag="LGJ20_004110"
     CDS             complement(9505..9705)
                     /gene="thiS"
                     /locus_tag="LGJ20_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBK2388113.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(9689..10444)
                     /gene="thiF"
                     /locus_tag="LGJ20_004111"
     CDS             complement(9689..10444)
                     /gene="thiF"
                     /locus_tag="LGJ20_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBK2388114.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(10437..11072)
                     /gene="thiE"
                     /locus_tag="LGJ20_004112"
     CDS             complement(10437..11072)
                     /gene="thiE"
                     /locus_tag="LGJ20_004112"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBK2388115.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGVSLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(11072..>12247)
                     /gene="thiC"
                     /locus_tag="LGJ20_004113"
     CDS             complement(11072..>12247)
                     /gene="thiC"
                     /locus_tag="LGJ20_004113"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBK2388116.1"
                     /translation="GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIL
                     RNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVP
                     MTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
                     IQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAP
                     FYTLGPLTTDIAPGYDHFTSGIGAEMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLIT
                     YKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYHDETLPQES
                     GKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEIYLRKEEA"
BASE COUNT         2907 a         3259 c         3318 g         2763 t
ORIGIN      
        1 tagaagacct ggtaacttgc cgtagcaaag gcgaatccag cttgttcagc cgcgaccagg
       61 ttgactacat ggacgtatcc acccagcagg tggtatccgt cggtgcgtcc ctgatcccgt
      121 tcctggaaca cgatgacgcc aaccgtgcat tgatgggtgc gaacatgcaa cgtcaggccg
      181 ttccgactct gcgcgctgat aagccgctgg ttggtactgg tatggaacgt gctgttgccg
      241 ttgactccgg tgtaactgcg gtagctaaac gtggtggtgt cgttcagtac gtggatgctt
      301 cccgtatcgt tatcaaagtt aacgaagacg agatgtatcc gggtgaagca ggtatcgaca
      361 tctacaacct gaccaaatac acccgttcta accagaacac ctgtatcaac cagatgccgt
      421 gtgtgtctct gggtgaaccg gttgaacgtg gcgacgtgct ggcagacggt ccgtccaccg
      481 acctcggtga actggcgctt ggtcagaaca tgcgcgtagc gttcatgccg tggaatggtt
      541 acaacttcga agactccatc ctcgtatccg agcgtgttgt tcaggaagac cgtttcacca
      601 ccatccacat tcaggaactg gcgtgtgtgt cccgtgacac caagctgggg ccggaagaga
      661 tcaccgctga catcccgaac gtgggtgaag ctgcgctctc caaactggat gaatccggta
      721 tcgtttacat tggtgcggaa gtgaccggtg gcgacattct ggttggtaag gtaacgccga
      781 aaggtgaaac tcagctgacc ccagaagaaa aactgctgcg tgcgatcttc ggtgagaaag
      841 cctctgacgt taaagactct tctctgcgcg taccaaacgg tgtatccggt acggttatcg
      901 acgttcaggt ctttactcgc gatggcgtag aaaaagacaa acgtgcgctg gaaatcgaag
      961 aaatgcagct caaacaggcg aagaaagacc tgtctgaaga actgcagatc ctcgaagcgg
     1021 gtctgttcag ccgtatccgt gctgtgctgg tagccggtgg cgttgaagct gagaagctcg
     1081 acaaactgcc gcgcgatcgc tggctggagc tgggcctgac agacgaagag aaacaaaatc
     1141 agctggaaca gctggctgag cagtatgacg aactgaaaca cgagttcgag aagaaactcg
     1201 aagcgaaacg ccgcaaaatc acccagggcg acgatctggc accgggcgtg ctgaagattg
     1261 ttaaggtata tctggcggtt aaacgccgta tccagcctgg tgacaagatg gcaggtcgtc
     1321 acggtaacaa gggtgtaatt tctaagatca acccgatcga agatatgcct tacgatgaaa
     1381 acggtacgcc ggtagacatc gtactgaacc cgctgggcgt accgtctcgt atgaacatcg
     1441 gtcagatcct cgaaacccac ctgggtatgg ctgcgaaagg tatcggcgac aagatcaacg
     1501 ccatgctgaa acagcagcaa gaagtcgcga aactgcgcga attcatccag cgtgcgtacg
     1561 atctgggcgc tgacgttcgt cagaaagttg acctgagtac cttcagcgat gaagaagtta
     1621 tgcgtctggc tgaaaacctg cgcaaaggta tgccaatcgc aacgccggtg ttcgacggtg
     1681 cgaaagaagc agaaattaaa gagctgctga aacttggcga cctgccgact tccggtcaga
     1741 tccgcctgta cgatggtcgc actggtgaac agttcgagcg tccggtaacc gttggttaca
     1801 tgtacatgct gaaactgaac cacctggtcg acgacaagat gcacgcgcgt tccaccggtt
     1861 cttacagcct ggttactcag cagccgctgg gtggtaaggc acagttcggt ggtcagcgtt
     1921 tcggggagat ggaagtgtgg gcgctggaag catacggcgc agcatacacc ctgcaggaaa
     1981 tgctcaccgt taagtctgat gacgtgaacg gtcgtaccaa gatgtataaa aacatcgtgg
     2041 acggcaacca tcagatggag ccgggcatgc cagaatcctt caacgtattg ttgaaagaga
     2101 ttcgttcgct gggtatcaac atcgaactgg aagacgagta attctcgctc aaacaggtca
     2161 ctgctgtcgg ggtaaaaccc ggcagcggat tgtgctaact ccgacgggag caaatccgtg
     2221 aaagatttat taaagtttct gaaagcgcag actaaaaccg aagagtttga tgcgatcaaa
     2281 attgctctgg cttcgccaga catgatccgt tcatggtctt tcggtgaagt taaaaagccg
     2341 gaaaccatca actaccgtac gttcaaacca gaacgtgacg gccttttctg cgcccgtatc
     2401 tttgggccgg taaaagatta cgagtgcctg tgcggtaagt acaagcgcct gaaacaccgt
     2461 ggcgtcatct gtgagaagtg cggcgttgaa gtgacccaga ctaaagtacg ccgtgagcgt
     2521 atgggccaca tcgaactggc ttccccgact gcgcacatct ggttcctgaa atcgctgccg
     2581 tcccgtatcg gtctgctgct cgatatgccg ctgcgcgata tcgaacgcgt actgtacttt
     2641 gaatcctatg tggttatcga aggcggtatg accaacctgg aacgtcagca gatcctgact
     2701 gaagagcagt atctggacgc gctggaagag ttcggtgacg aattcgacgc gaagatgggt
     2761 gcggaagcaa tccaggctct gctgaagagc atggatctgg agcaagagtg cgaacagctg
     2821 cgtgaagagc tgaacgaaac caactccgaa accaagcgta aaaagctgac caagcgtatc
     2881 aaactgctgg aagcgttcgt tcagtctggt aacaaaccag agtggatgat cctgaccgtt
     2941 ctgccggtac tgccgccaga tctgcgtccg ctggttccgc tggatggtgg tcgtttcgcg
     3001 acttctgacc tgaacgatct gtatcgtcgc gtcattaacc gtaacaaccg tctgaaacgt
     3061 ctgctggatc tggctgcgcc ggacatcatc gtacgtaacg aaaaacgtat gctgcaggaa
     3121 gcggtagacg ccctgctgga taacggtcgt cgcggtcgtg cgatcaccgg ttctaacaag
     3181 cgtcctctga aatctttggc cgacatgatc aaaggtaaac agggtcgttt ccgtcagaac
     3241 ctgctcggta agcgtgttga ctactccggt cgttctgtaa tcaccgtagg tccatacctg
     3301 cgtctgcatc agtgcggtct gccgaagaaa atggcgctgg agctgttcaa accgttcatc
     3361 tacggcaagc tggaactgcg tggtcttgct accaccatta aagctgcgaa gaaaatggtt
     3421 gagcgcgaag aagctgtcgt ttgggatatc ctggacgaag ttatccgcga acacccggta
     3481 ctgctgaacc gtgcaccgac tctgcaccgt ctgggtatcc aggcatttga accggtactg
     3541 atcgaaggta aagctatcca gctgcacccg ctggtttgtg cggcatataa cgccgacttc
     3601 gatggtgacc agatggctgt tcacgtaccg ctgacgctgg aagcccagct ggaagcgcgt
     3661 gcgctgatga tgtctaccaa caacatcctg tccccggcga acggcgaacc aatcatcgtt
     3721 ccgtctcagg acgttgttct gggtctgtac tatatgaccc gtgactgtgt taacgccaaa
     3781 ggcgaaggca tggtgctgac tggcccgaaa gaagcagaac gtctgtatcg ctctggtctg
     3841 gcttctctgc atgcgcgcgt taaagtgcgt atcaccgagt atgaaaaaga tgcaaacggt
     3901 gaattagtag cgaaaaccag cctgaaagac acgactgttg gccgtgccat tctgtggatg
     3961 attgtaccga aaggtctgcc ttactccatc gtcaaccagg cgctgggtaa aaaagcaatc
     4021 tccaaaatgc tgaacacctg ctaccgcatt ctcggtctga aaccgaccgt tatttttgcg
     4081 gaccagatca tgtacaccgg cttcgcctat gcagcgcgtt ctggtgcatc tgttggtatc
     4141 gatgacatgg tcatcccgga gaagaaacac gaaatcatct ccgaggcaga agcagaagtt
     4201 gctgaaattc aggagcagtt ccagtctggt ctggtaactg cgggcgaacg ctacaacaaa
     4261 gttatcgata tctgggctgc ggcgaacgat cgtgtatcca aagcgatgat ggataacctg
     4321 caaactgaaa ccgtgattaa ccgtgacggt caggaagaga agcaggtttc cttcaacagc
     4381 atctacatga tggccgactc cggtgcgcgt ggttctgcgg cacagattcg tcagcttgct
     4441 ggtatgcgtg gtctgatggc gaagccggat ggctcgatca tcgaaacgcc aatcaccgcg
     4501 aacttccgtg aaggtctgaa cgtactccag tacttcatct ccacccacgg tgctcgtaaa
     4561 ggtctggcgg ataccgcact gaaaactgcg aactccggtt acctgactcg tcgtctggtt
     4621 gacgtggcgc aggacctggt ggttaccgaa gacgattgtg gtacccatga aggtatcatg
     4681 atgactccgg ttatcgaggg tggtgacgtt aaagagccgc tgcgcgatcg cgtactgggt
     4741 cgtgtaactg ctgaagacgt tctgaagccg ggtactgctg atatcctcgt tccgcgcaac
     4801 acgctgctgc acgaacagtg gtgtgacctg ctggaagaga actctgtcga cgcggtaaaa
     4861 gtacgttctg ttgtatcttg tgacaccgac tttggtgtat gtgcgcactg ctacggtcgt
     4921 gacctggcgc gtggccacat catcaacaaa ggtgaagcaa tcggtgttat cgcggcacag
     4981 tccatcggtg aaccgggtac acagctgacc atgcgtacgt tccacatcgg tggtgcggca
     5041 tctcgtgcgg ctgctgaatc cagcatccag gtgaaaaaca aaggtagcat caagctcagc
     5101 aacgtgaagt cggttgtgaa ctccagcggt aaactggtta tcacttcccg taatactgaa
     5161 ctgaaactga tcgacgaatt cggtcgtacc aaagaaagct acaaagtacc ttacggtgcg
     5221 gtactggcga aaggcgatgg cgaacaggtt gctggcggcg aaaccgttgc aaactgggac
     5281 ccgcacacca tgccggttat caccgaagta agcggttttg tacgctttac tgacatgatc
     5341 gacggtcaga ccattactcg tcagacagac gaattgaccg gtctgtcttc gctggtggtt
     5401 ctggattccg cagaacgtac agcaggtggt aaagatctgc gtccggcact gaaaatcgtt
     5461 gatgctcagg gtaacgacgt tctgatccca ggtaccgata tgcctgcgca gtacttcctg
     5521 ccgggtaaag cgattgttca gctggaagat ggcgtacaga tcagctctgg tgacaccctg
     5581 gcgcgtattc cgcaggaatc cggcggtacc aaggacatca ccggtggtct gccgcgcgtt
     5641 gcggacctgt tcgaagcacg tcgtccgaaa gagccggcaa tcctggctga aatcagcggt
     5701 atcgtttcct tcggtaaaga aaccaaaggt aaacgtcgtc tggttatcac cccggtagac
     5761 ggtagcgatc cgtacgaaga gatgattccg aaatggcgtc agctcaacgt gttcgaaggt
     5821 gaacgtgtag aacgtggtga cgtaatttcc gacggtccgg aagcgccgca cgacattctg
     5881 cgtctgcgtg gtgttcatgc tgtgactcgt tacatcgtta acgaagtaca ggacgtatac
     5941 cgtctgcagg gcgttaagat taacgataaa cacatcgaag ttatcgttcg tcagatgctg
     6001 cgtaaagcta ccatcgttaa cgcgggcagc tccgacttcc tggaaggcga acaggttgaa
     6061 tactctcgcg tcaagatcgc aaaccgcgaa ctggaagcga acggcaaagt gggtgcaact
     6121 tactcccgcg atctgctggg tatcaccaaa gcgtctctgg caaccgagtc cttcatctcc
     6181 gcggcatcgt tccaggagac cactcgcgtg ctgaccgaag cagccgttgc gggcaaacgc
     6241 gacgaactgc gcggcctgaa agagaacgtt atcgtgggtc gtctgatccc ggcaggtacc
     6301 ggttacgcgt accaccagga tcgtatgcgt cgccgtgctg cgggtgaagc tccggctgca
     6361 ccgcaggtga ctgcagaaga cgcatctgcc agcctggcag aactgctgaa cgcaggtctg
     6421 ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg
     6481 gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa ggaatttctg
     6541 aatactcata atcaatgtag agatgactaa tatcctgaaa ctgactgaac taattgagtc
     6601 aaactcggca aggattcgat actattcctg tgtaactttc ttaaggaacg agaatgaaac
     6661 aggaagtgga aaagtggcga ccttttggac atccggatgg tgatattcgt gatttatcat
     6721 ttcttgatgc tcatcaggct gtctacgttc agcatcatga gggcaaagag cctttagagt
     6781 atcgcttttg ggttacctac tctcttcact gcttcacaaa agattatgaa catcagacga
     6841 acgaagaaaa acaatcgtta atgtaccacg cgcctaaaga atctcgtccc ttctgccagc
     6901 accgttataa cttagcgcgc acacacttaa aaagaactat tttggcgctg ccagaaagca
     6961 acgttattca tgccgggtat ggtagctatg ccgtgattga ggtggactta gacggaggag
     7021 ataaggcatt ttactttgtt gcgttcaggg ctttcaggga aaagaaaaaa ctccgtttgc
     7081 atgtaactag cgcttatccc atttctgaaa aacagaaagg taaatcagtg aaatttttca
     7141 ccattgccta caacttattg agaaataagc agcttcctca gccctcaaaa taacaaaacc
     7201 caccttaagg tgggtttcgc cagagaatta tctctggtat tcagaacgcc attaccggac
     7261 tttgccttga ccttgcgata atcgcaggtt gcgggatgtc tgaatttctt cagtctgctg
     7321 catcctggaa gatgagaaca tgtgttctta ttttcgtctc tatcatagtt gagtatttac
     7381 tctcttacaa tcagatctct ttcattgctc aacaggcgat ggcttcagac tttgcattac
     7441 ggaattttta agaaaggcag ggcgaaacga ggaagaagct tttcgccctg gacaacattc
     7501 ctgctgacgg cactaccata aaaaaacaca acgctttcaa caaccttgcg tttttcatct
     7561 tccgtagcgg ctcggaataa atttacgttc ttacattccg tctcatagtc tttgcgaggc
     7621 ccgtcccaga tagctgtccc agtctttcca taccggctgc aaaccctgag cgattaacgc
     7681 ggcagcaacc gcttccggtc tgcgatcgtc gtgcggtgag aactgttcca actcggggtg
     7741 attatcggca tagccacctg gctgcgtttt cgagaaggcg ctgacgttat tgatcgccag
     7801 cggaataacg cgatcgcgaa accacggtga ttcccgcgtg gagagtgaca gttcaatctc
     7861 cggtgcaagc agtcggaagg cgcagatggt ttgcactaac tggcgttcat ccataatcga
     7921 cgcaggctca atgccgccag tacacgggcg caggcgcgga aaggagacag agtaacggct
     7981 ttgccagtaa tgctgttgca gccatagcaa atgttcggca accatatagc agtcaacgcg
     8041 ccagttgtcg gaaaggccaa ttagcgcgcc gaggcctatc ttatcaatcc ccgcacgccc
     8101 cagccgatcc ggcgtttcca gccgccagaa gaagtcctgt ttttttcctt tcagatgatg
     8161 gcgggcataa gtcgcctcgt gatatgtctc ctgataaacc atcacgccat ccagaccaag
     8221 ctgctttaac tcggcgtatt ccgtctccgc cagcggttgc acttccattt gtagtgaaga
     8281 gaactgttca cgcagggcgg ggagatgacg acgaaagtaa tccatcccca ctttcgcctg
     8341 atgttcacca gtgactaata gcagatgttc aaagcccatc tcccgtatag cggcgctttc
     8401 cctggcaata tccgcttcat ccagcgtttt gcgcttgatg cgattactca tggaaaatcc
     8461 gcagtacgtg cagtcgttag cgcaaagatt ggaaagataa agcgggacgt agaaactaac
     8521 tgtgttgcca aatcgctgac gggtcagacg ctgcgcccgt tgggccagtt gttccagata
     8581 gccactggcg gctggcgata acagcgccat catatcgtcg cgggtgaatt gcgaggcatt
     8641 tagcgcccgc tctacgtcaa cagccgtttt gccgttgata cgcaggcgga tgtcgtccca
     8701 gtccagttgt cgccagcgat cgctgaaggt tttcatgccg atgcctccag aaatccagtc
     8761 agcgggctgg tggcatgagc aaaatgactg cggctgcccg gtccggactg acgtgccagc
     8821 aggcctgctt ctaccgccag acgaaatgcc ttcgccatgt tgacgggatc gtccgcgacg
     8881 gcaattgccg tattcactaa caccgcgtcg gcccccattt ccagcgcctg cgcggcatgg
     8941 ctgggaacgc cgatgccagc atcgacaacc accagcactg tggcctgctg gataataatc
     9001 tccagcatgg cgcgggtttc cagtccctga ttcgagccaa tcggcgcgcc gagcggcatc
     9061 accgctgcac agccgacttc ttccagacgt ttacacaata ccggatcggc cccgcagtaa
     9121 ggcagcacga caaatccctg ttgtaccagc gtttcggcgg ctttcagggt ttcgatggga
     9181 tcgggcaaca gccagcgggc gtcagggtga atctctaatt ttaaccagtt tgtgcctaac
     9241 gcttcacgag ccagatgggc ggcgaaaatg gcttcttccg ctgttttcgc cccggatgta
     9301 tttggcagca gggtcacacc cgccgcgata agcggttcga ggatagcgtc gttgtgctgg
     9361 cgcaagtcga cacgtttcat cgccagtgtc accagctggc tgccggaagc gcggatcgcc
     9421 tccaccatca gttgtgaaga agcgaatttc cctgtgccgg taaacagatg tgaatcaaac
     9481 gttttgtccg caatacgtaa catttcaacc ccctgcaata acctgaaaaa gcaggatctg
     9541 gtcgccatcc tgcacgatat gttgcgccca ctgctcacgc gggacgattt gctgattaat
     9601 cgccagagcc gcgcccgctt gtcgttggtc gagttgctcc agtagttcgt gaacagtttg
     9661 cccggcggcg cactgcatcg gttgatcgtt aaacaggatc tgcattgctt cctccgcata
     9721 ccgggcaacc actggcgcgg cgcaacgcca ggctgcgcca ctggctcgat ttaccgtcga
     9781 acagtcggag ttctcccgca ggtgtctcta taccgcttaa taacttaatg gcttccagtg
     9841 cctgcaaagt gcccataacc ccgaccaccg ggccaaccac gcccgccgtg cggcagttgc
     9901 gttctggctc ctggttatct ggccacaggc agcggtaaca cccctgctcc cagggcggcg
     9961 tcagtaccat caactgaccg ccaaatccga ccgcgctggc agtgataagc ggcgtgttga
    10021 gtgccacgca ggcggcatta atctcctggc gagtcgccat attgtcggta cagtcgagca
    10081 ccacatcggc ccgtgcaacc gcatctttta acgcctcacc cgttaaccgt tgttgtaatg
    10141 ctgtcagttg aatgtcggga ttcaactgtg tcagtcgctg ttggctgacc tgcgatttcg
    10201 ggcgatcgat atcttcagtg gtaaagagga tttgtcgttg cagattgctt aaatgcacat
    10261 cgtcgtcatc tgccagtacc agcgtcccga cgccagcgcc cgccaggtat agcgcagcag
    10321 gtgtacccag cccgccaaga ccgataatca gcacctggct gtcgagcagt ttttgctgcc
    10381 cgtcaagagc gatatcgtcg agcaggattt ggcggctata acgcataaaa tcacggtcat
    10441 tcatcgccaa ctcctgcaat ttccagcaac tgtgccgttg ccaaacgcca gtctgcggct
    10501 tgagtaatgg cgctgacgac ggcgatactg ccgacacccg ttgctatcac cgcaggcgcg
    10561 cgtgccagac tgacaccgcc aatcgccacg gtgggataat ccgccagtcg ctcaacatgc
    10621 cgtgccagct gttccagccc ctgcggtgca gaaggcatct gtttggtttg cgtcgggaac
    10681 acatgtccca gcgcgatata agaggggcgt gctgccagcg cgacgtcgat ttccatatcg
    10741 tcatgtgtcg aaacacccag tcgcaggcct gccgcgcgga tggcattgag atcggtggct
    10801 tgcaaatctt cctgccccaa atggacgcca tacgcctgat gcttgatcgc gagccgccag
    10861 taatcgttga taaacaatcg cgcgttatag cggcggccca gcgcaattgc cgccacgaca
    10921 tcggcttcca cctcttcatc gcgccgatct ttgatgcgta gctggagagt acgtacgcct
    10981 gcatccaaca gacgttcgat ccactgtacg ctgtccacca ctgggtacag tcctaaacga
    11041 aaaggtacag gaggaaaatc aggctgatac atcacgcttc ctccttacgc aggtagattt
    11101 ctccgcctct ggcacggaag ttctccgaca tatccgccat tcccatttca atagtttgcg
    11161 tggcggcgta atcacgcact tcctggctga ttttcatcga gcagaatttc ggcccacaca
    11221 tggagcaaaa atgggcgact ttacctgact cttgcggcag ggtttcatcg tgataagcgc
    11281 gggcggtaaa cgggtcgagg gccagattaa actggtcttc ccagcgaaat tcgaagcggg
    11341 ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg ccctttcgcc aggtcagcgg
    11401 cgtgggcggc aatcttatag gtgataagcc cctgcttaac atcttcttta ttgggcagac
    11461 ccagatgctc ttttggcgtt acgtaacaga gcatcgcgca gccaaaccag ccaatcatct
    11521 ccgcaccaat ccccgacgtg aagtggtcat agcccggcgc aatatcggta gttagcggcc
    11581 ccagagtgta aaacggcgct tcgtggcagt gctctaactc ctcggtcata ttgcggcgga
    11641 tcatctgcat cggcacgtgg cctgggcctt caatcatcac ctgcacgtca tattcccagg
    11701 caattttggt cagttcgccc agcgtatgca gctcggcaaa ctgcgcttca tcgttggcgt
    11761 cctgaataga accggggcgc agaccgtcgc ccagcgacag cgaaacgtca taagcggcac
    11821 agatttcaca aatttcgcgg aagtgttgat agaggaaatt ttcctgatga tgggagaggc
    11881 accatttcgc cataatcgaa ccgccgcgag agacgatacc ggtcaggcgt tttgcggtca
    11941 tcggcacata gcgcagcagt acgcccgcat ggatagtgaa gtaatccaca ccttgctcgg
    12001 cctgttccag cagcgtgtcg cggaacgctt cccaggtaag atcttcggcg atcccgttaa
    12061 ccttctccag cgcctggtag atcggcactg taccgatcgg caccgggctg ttacgcaaaa
    12121 tccactcgcg ggtttcgtga atatagcgac cggtggagag atccatcacc gtatccgctc
    12181 cccagcgcgt ggaccatacc agcttttcca cttcttcttc gatggaagag gtgaccgccg
    12241 agttgcc
//
LOCUS       DAFATA010000123        12213 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFATA010000123 DAFATA010000000
VERSION     DAFATA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.124"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..530
                     /gene="fadI"
                     /locus_tag="LGJ20_004114"
     CDS             <1..530
                     /gene="fadI"
                     /locus_tag="LGJ20_004114"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="FadI; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadJ; similar to
                     FadA2B2 complex; functions in beta-oxidation of fatty
                     acids during anaerobic growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBK2388117.1"
                     /translation="RKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYA
                     FTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFA
                     REVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELHRRGGGFGLVTA
                     CAAGGLGAAMVLEAE"
     gene            530..2674
                     /gene="fadJ"
                     /locus_tag="LGJ20_004115"
     CDS             530..2674
                     /gene="fadJ"
                     /locus_tag="LGJ20_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBK2388118.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            2877..3362
                     /gene="sixA"
                     /locus_tag="LGJ20_004116"
     CDS             2877..3362
                     /gene="sixA"
                     /locus_tag="LGJ20_004116"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBK2388119.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            4037..4600
                     /locus_tag="LGJ20_004117"
     CDS             4037..4600
                     /locus_tag="LGJ20_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2388120.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            4682..7324
                     /gene="yfcU"
                     /locus_tag="LGJ20_004118"
     CDS             4682..7324
                     /gene="yfcU"
                     /locus_tag="LGJ20_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HBK2388121.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            7344..8096
                     /locus_tag="LGJ20_004119"
     CDS             7344..8096
                     /locus_tag="LGJ20_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBK2388122.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            8112..8621
                     /locus_tag="LGJ20_004120"
     CDS             8112..8621
                     /locus_tag="LGJ20_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388123.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILIDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            8618..9106
                     /locus_tag="LGJ20_004121"
     CDS             8618..9106
                     /locus_tag="LGJ20_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388124.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            9103..9642
                     /locus_tag="LGJ20_004122"
     CDS             9103..9642
                     /locus_tag="LGJ20_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388125.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            9644..10495
                     /locus_tag="LGJ20_004123"
     CDS             9644..10495
                     /locus_tag="LGJ20_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBK2388126.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(10568..11119)
                     /gene="smrB"
                     /locus_tag="LGJ20_004124"
     CDS             complement(10568..11119)
                     /gene="smrB"
                     /locus_tag="LGJ20_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBK2388127.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            11285..>12213
                     /gene="prmB"
                     /locus_tag="LGJ20_004125"
     CDS             11285..>12213
                     /gene="prmB"
                     /locus_tag="LGJ20_004125"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK2388128.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYK"
BASE COUNT         3027 a         2995 c         3239 g         2952 t
ORIGIN      
        1 atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat ggcgcggcag
       61 cggtgatcct gatgactgaa tcccgagcga aagaattagg gctggtgccg ctggggtatc
      121 tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc ggtccggcct
      181 ggtcaacacc gctggcgctg gagcgtgccg gtttgacgat gagcgatctg acattgatcg
      241 atatgcacga agcctttgcc gctcagacac tggcgaatat tcagttgctg ggcagcgaac
      301 gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac gatagcaaat
      361 ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc ggcgcgcgga
      421 tgattaccca gacattgcat gaacttcacc gtcgcggcgg tggatttggt ttagttaccg
      481 cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa tggaaatgac
      541 atcagcgttt acccttaatg ttcgtctgga caacattgcc gttatcacca tcgacgtacc
      601 gggtgagaaa atgaataccc tgaaggcgga gtttgcctcg caggtgcgcg ccattattaa
      661 gcaactccgt gaaaacaaag agttgcgagg cgtggtgttt gtctccgcta aaccggacaa
      721 cttcattgct ggcgcagaca tcaacatgat cggcaactgc aaaacggcgc aagaagcgga
      781 agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc ccattccggt
      841 tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc tggcgtgcca
      901 cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag tacaacttgg
      961 attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg tcagtacagc
     1021 attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa agctggggct
     1081 ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc tggcgaagaa
     1141 ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg ggccgttagg
     1201 tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc aaggcaatta
     1261 tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg gcaccagcag
     1321 cggttatgac gccgaagctc gggcgtttgg cgaactggcg atgacgccac aatcgcaggc
     1381 gctgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta gtgatgcacc
     1441 gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg gcggtattgc
     1501 ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca acccgcaggg
     1561 cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc gccgtcgtca
     1621 tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga cggactatcg
     1681 cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg aattgaaaca
     1741 acagatggtg gcggaagttg agcaaaatag cgccgctcat accatctttg cttcgaatac
     1801 gtcatcttta ccgattggtg atatcgccgc tcacgccacg cgacctgagc aagttatcgg
     1861 cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc ctcatgcggg
     1921 tacatcagcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg gcaaaacgcc
     1981 cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc cttacattaa
     2041 tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg ccgcgttagt
     2101 gaaatttggt tttccggtag gtccaatcca acttttggat gaggtaggaa tcgacaccgg
     2161 gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg cgcctgcaaa
     2221 tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc ggggtttcta
     2281 tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca tttacccgct
     2341 cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac ggtgtgtgat
     2401 gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta gcgtgcgtga
     2461 cggggatatt ggtgcggtat ttggcattgg ttttccgcca tttctcggtg gaccgttccg
     2521 ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac ttgccacgca
     2581 gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc gtggggaaag
     2641 tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata tgaatccgcg
     2701 ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta cgccattgag
     2761 gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca ccgttttctt
     2821 tctctggttt cagatgaaag aaaacgggcg aatctggtta acaaaagcgg tgcaatatgc
     2881 aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat tccgttcgtc
     2941 ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg aaaggtcaaa
     3001 aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa acactggaag
     3061 aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag ttaacgccct
     3121 gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa ggtgttgctt
     3181 cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag ttatgtccgg
     3241 gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt gatgaaagcg
     3301 gaaacggtac attcaactgg cagatgagcc cgtgtaatct gaagatggca aaagctatct
     3361 gatgtgttcc aaattatgcc caatgaggta gattcattgg gcataattgc tcatcaggca
     3421 gcggatatct gtcaacctca ctactatcga agactctcct tatctataaa taaaacactt
     3481 tctacattca ttcggaaatt attcttctgc gcgtatcttg attttgccca aagttacatg
     3541 ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga taatagagtt
     3601 gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt gtttatatat
     3661 tatattgtca aaagtggata aatgccaatc gcattatatg ttcattgttt gttttttaag
     3721 ctgttgatat tcattattga tatactttct attttttcta aatacttatt ccgtattttt
     3781 taaggatgtc cttaagataa cctttcttct gtcaatatat taattaaatc tacatttact
     3841 ctttagtaaa attaaattgc atcaggggtg tggaaacatc actatgcggc cccacaccaa
     3901 cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca atggctgggt
     3961 ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt aattatttat
     4021 agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg caatggtgat
     4081 gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg cacgttttta
     4141 cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac tggaagttga
     4201 tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc cgaaaagttt
     4261 ccagattcgt ctgcaagatt gcgtatttga tactcaggaa acaatgacca ctacctttac
     4321 tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata ccgataccgg
     4381 tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct cttacaaaag
     4441 cggcatgggt attgaccaga aaatagtgaa agacacttct accaataaag gcaaagcgaa
     4501 gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag atctgggtaa
     4561 tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc catagcgaca
     4621 ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga tagaaacgtg
     4681 tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg cgctggcaat
     4741 gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt cccgttttct
     4801 ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt tccagccagg gatatgttga
     4861 gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg aagagtacga
     4921 tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc tgacaccgga
     4981 actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat ggagccacga
     5041 tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg atttaagcca
     5101 gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttacc ccgactggga
     5161 tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca gcatcaacgc
     5221 acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg gcaacgggac
     5281 ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta actatcaaca
     5341 tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa aatgggagtg
     5401 gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg cgctgggcga
     5461 ggattacctc agatccgata tttttgatgg ttttaactat gttggtggca gtgtcagtac
     5521 tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt ccggcgtggc
     5581 acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg aaacgcaggt
     5641 tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta cgttgcatat
     5701 tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg cctcgatgcc
     5761 atacctcact cgcccaggtc aggttcgcta taagatcatg atgggccgtc cgcaagagtg
     5821 gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg ggatcgctaa
     5881 cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg cggcgcttgg
     5941 cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc actcgcacac
     6001 caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact ccttccgtgt
     6061 gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg gatatcgctt
     6121 ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca gcggaatggt
     6181 acgcacgggc aacgacaaag agatgtacac cgccacttat aaccagaact tccgcgatgc
     6241 gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg aggagcagac
     6301 aaactacaac atcatgctct cgcactattt caatatgggc agtattcgta atgtcagcat
     6361 ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca tgtacatttc
     6421 gctcagtatg ccgtggggcg acaacagtac cgttagctat aacggtaact atggcagtgg
     6481 gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc actatcagtt
     6541 gaacgtcggc accagtgaca aacacaccag cgttgacggc tattacagcc atgatggttc
     6601 gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct ctgcgggcct
     6661 gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc gtacccagaa
     6721 tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc cggtggaagg
     6781 taacggggct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg acgtcaataa
     6841 ctattaccgc aatcaggcgt atatcgacct caacagattg cctgaaaacg ctgaagcaac
     6901 ccagtcggtg gtgcaagcca cgctaactga aggtgccatt ggctaccgta aatttgctgt
     6961 cattagcgga caaaaagcga tggcggtgct gcgcctgagc gacggcagcc atcctccgtt
     7021 tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg atgacggcaa
     7081 tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga gtggtgttgc
     7141 gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg gcttgttact
     7201 gccatgccag cataaaggca atgtagcacc tatcacttcg ccggcggtca aaccggcgat
     7261 tcaggaacag acacagcggg tgacgccaac ggaacctccg acttcaattt cagtaaacca
     7321 gtaacgtgat taaggaatga tccatgttta atctgactaa cactgcaaaa atcgttgtcc
     7381 cggcactggc gctgctggcg acagcggtca gtttctccag ccacgcttcg gtgacgcccg
     7441 acaggacacg gctggtgttt aacgaaagcg acaagtccat cagcgtcacg ctgcgcaaca
     7501 acgatcccac actgccttat ctggcgcaga gctggataga ggatgcaaag ggcaacaaaa
     7561 tcagctcgcc gcttgcggtg cttccgccag tgcagcgcat cgatgcgatg atgaacggcc
     7621 aggtgaaggt gcaggggctg ccggatatca acaaactgcc ggccgaccgc gagagcgtgt
     7681 tctatttcaa cgtgcgcgaa attccgccga aatcaaataa accgaacaca ctgcagattg
     7741 cgctgcagac gcgtatcaaa ctgttctggc gaccgaaggc gctggagaac accagcatga
     7801 aaagtccgtg gcagcataag gtgacgctca tccgtaacgg ccaggacttc accgtcaata
     7861 acccgacgcc gtactacgtg atcatcagca acgccagcgc taaaaaaggc ggcaacccgc
     7921 cggcgggatt cacaccgctg atgatggagc cgaaaacctc cgcaccgctg aatgcgaaga
     7981 tggacagcgt gccggtgctg acttacatca acgactacgg cgcgcggatg ccgctgtact
     8041 tcatatgcac cggcaacagc tgccaggtgg atgaagagca gagccgcaaa ggctgatgcc
     8101 gggagggact tatgagtcac aagtttgtag cgggcgcagg gttgctggtg ctggccggat
     8161 ggggcatcag cggtttggct gttgctggca gtgatatcaa acaggtggat ctgacgctgc
     8221 gaatcctgat cgatggaccg ccgccttgct ccgtgaaggg aagcgctgtg aaatttggca
     8281 atgtgataat caacaggatc gatggtacca attaccaaca ggatgcgaaa tacaccctca
     8341 actgcggtaa taaagtcagc gatgacttac ggatgcagct caaaggcaat accgccgtga
     8401 tcaacggaga aacggttctc agcacggata tcgccggcct gggggtgcgg atagaaaacg
     8461 ccgccgacaa ctccctgttt gcagttggtg aaaataactg gacgcctttt aatgtcaaca
     8521 ctcagcctca gctgaaagcg gtgccggtaa aacagagcgg ggtgcaactg acgccgtcgg
     8581 agtttaacgc cacgatgacc atggtggtgg attaccaatg aaaaaattcc tgctcctttt
     8641 actgtgcgct ggttgcgggg ctgtgctggc ggcggaagat gacatcacat tccacggtac
     8701 gctgatgtcg ccgcccgcct gtagcatcag cggcgggcaa accatcgaag tgcaatttcg
     8761 cgatcttatt atcgacagca ttgatggcaa ttatggtcgt cagaaagtgg actacgaact
     8821 gagctgcgac tcggatgtcc gcgatcccga gtggaatatg acactctcct ggacggggac
     8881 tgccacctcc tacaacgact ctgctattga gacggatgta ccgggcttcg gaattgagct
     8941 tcagcatgac ggtcagcgtt ttaagctcaa cgagccgctt tccattaacg ccaccgattt
     9001 tagccagaag ccaaagctgg aggcggtacc ggtcaaagcg gctgatgcgg tgctgacgga
     9061 cacgaatttt tctgcttacg caaccttacg ggtggattac caatgaaaac agcacacatt
     9121 tattcacccg gttatgcgct gttagcaggc ctgctgctga ccgccagcac gggggcacgg
     9181 gcagtcgata acaacctgca tttttacggc aacctgctga gtaaatcctg cacgctggtg
     9241 gtggatggtg ccaatctggc ggaaattcat tttcccaccg tcagcaaccg ggatttgatg
     9301 gtcgccgggc agtccgcgca tctgccggtt gtgtttaagc tgaaagactg caaagggccg
     9361 gcgggataca acgtcaaagt caccctcacc ggaacggaag acagcggtca gccgggtttt
     9421 ctggcagtcg atgacacctc caccgcgcag ggcgtgggga tagggatgga aacgctcgac
     9481 ggcaagcggg tggatattaa caacaccacc ggggcgactt ttgcgcttac caacggcaac
     9541 aatgatatta atttccgggc ctggatccag gcgaaaagcg gtcgtgatgt aaccatcggt
     9601 gatttcaccg ccagcctgac agctacgttt gagtatatat aaaatgcata aaatacgtca
     9661 tgttatatgt ttattggtgc tggctctgtg cgcttctggg atacaggctg cgacaaagat
     9721 cgccacactc tatgtacccg cggggacgac cagcgttgtt gcaaagtatc gttttcatct
     9781 ttcagtcctg acccctcaat cagttgaata tggcacgtat gaatctaatt caactgcggc
     9841 agcaagtttg cctttagtta gttggaccgg gagtcctccc ggccctgaac tcaggatgga
     9901 gcgcaataat acaaccttac cggattctac ctgccccgga ttagaagagt atgatgctct
     9961 ctctccagta acggcctggt catgtaatga attagtactt ggcgtttatt acgatggaga
    10021 tttacacggt tgtccctgga ttgtgtcgtc ttatgttgaa agcgccagta ctatggatca
    10081 gcgtttcggg gccggaattt ttataggacc aacaaaagtt agctctgact gcccgacaga
    10141 gtccctggca ccgtatgatg tttcctggaa tgaaaattat gttgtacata acaaagtcgt
    10201 caggttgcag tcaacaggtg gtgttattga gcaaacactg cccactttcc tgatggaaaa
    10261 cggcaaactg tgtgatggca gtaattttga cgagcgtgga gcctactgcc gttttgtcgc
    10321 acagcagatg accttctcga cttccggctg tgataatgcc aaagtgacgg tgacgccaga
    10381 gccgcaaccg atcacgtcca ggcaactgca tgatatgaag ctacgcgtgg acacgacatc
    10441 tcgacagccc atagattcta cctgccgatt cacttacata ctgaatatgt attagcgcac
    10501 cttttctcac ctggcagggt cattcgcccc tgccattccg cgctcctggc acaacacgct
    10561 caactcctca gggcaactcc ggcggcaacc actcttccac ttcaatcaac accaacaaag
    10621 ccgcatcacc gccgtactct ttcggtgcct ggtgaaatgc catcacatgc ggatgttgtg
    10681 ccagccacag cggtgtttgt tgcttcaaaa tatgcttccc gtgcccatgc atcacgcagg
    10741 cacaaaacac atgttcgcgg cgacaggcgg caatcaacgc gcccagttcc tgcttggcct
    10801 gcagctgcgt cagaccgtgc aaatccaaaa acaactccgg cgaataatcg ccacggcgca
    10861 gtttcttcgc ctcaaaatgg ctgacatccg ggcgaacata tttgaccgga ccttcggtat
    10921 tcagtaacgg ctgaaactca tcggagaaat aatggctggc atcagcctgc tcctgaatca
    10981 accgcttcac cggcacttcg ctgacttttt tacgctgcgg tcggtggaca atcgtgtcct
    11041 gcttaatctt gcgagtcccc gccatcaact ggcgaaacag agcctggtcc tcctcgctaa
    11101 gtgttgtttt ctttttcatt tatctgtctc tatttctaat tttcctacag tttacccgac
    11161 tcgtagcgca atcgatcgtt ttaaacgtat ttttcctcac tcaccgcgcg tgaatgctga
    11221 tttatcgtca tcttcatggc aaactagccg ccgaaattat tgcgatcatg ccctggagga
    11281 atacgtggat aaaattttcg ttgatgaagc agtaaatgag ctgcaaacca ttcaggacat
    11341 gttgcgctgg tcggtgagcc gcttcagcgc ggcgaatatc tggtacggcc acggcactga
    11401 caacccgtgg gatgaagccg tgcaactggt gttgccttcg ctctacctgc cgttggatat
    11461 cccggaagac atgcgcaccg cgcgtctgac ctccagcgaa aaacaccgta ttgttgagcg
    11521 cgtgattcgc cgcgtcaacg agcgcattcc ggtggcctac ctgaccaaca aagcctggtt
    11581 ctgtggccat gaattttacg tcgatgaacg cgtgctggtg ccgcgctcgc cgattggtga
    11641 actgatcaac aataaatttg ccggacttat cagcaagcaa ccgcagcata ttttagatat
    11701 gtgtaccggt agtggctgca tcgccattgc ctgtgcttat gccttcccgg aagcggaagt
    11761 cgacgcggta gatatctctc ccgacgcgct ggcggttgcc gaacagaaca tcgaagaaca
    11821 cggtctgatc cacaacgtca ttccgattcg ttccgatctg ttccgcgact tgccgaaagt
    11881 gcagtacgat ctgattgtca ctaacccgcc gtatgtcgat gcggaagata tgtccgacct
    11941 gccaaatgaa taccgccacg agccggaact gggcctggca tctggcaccg acggcctgaa
    12001 actgacgcgt cgcattctcg gtaacgcggc agattacctt gctgatgatg gtgtgctgat
    12061 ttgtgaagtc ggcaacagca tggtacatct tatggaacaa tatccggatg ttccgttcac
    12121 ctggctggag tttgataacg gcggcgatgg tgtgtttatg ctcaccaaag agcagctgct
    12181 ggcggcccgt gaatatttcg cgatttataa aga
//
LOCUS       DAFATA010000124        11965 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFATA010000124 DAFATA010000000
VERSION     DAFATA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.125"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(101..1561)
                     /locus_tag="LGJ20_004126"
     CDS             complement(101..1561)
                     /locus_tag="LGJ20_004126"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBK2388129.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(1642..2826)
                     /gene="uxuA"
                     /locus_tag="LGJ20_004127"
     CDS             complement(1642..2826)
                     /gene="uxuA"
                     /locus_tag="LGJ20_004127"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK2388130.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            3166..4509
                     /gene="gntP"
                     /locus_tag="LGJ20_004128"
     CDS             3166..4509
                     /gene="gntP"
                     /locus_tag="LGJ20_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBK2388131.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(4752..5654)
                     /gene="fimH"
                     /locus_tag="LGJ20_004129"
     CDS             complement(4752..5654)
                     /gene="fimH"
                     /locus_tag="LGJ20_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBK2388132.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(5674..6177)
                     /gene="fimG"
                     /locus_tag="LGJ20_004130"
     CDS             complement(5674..6177)
                     /gene="fimG"
                     /locus_tag="LGJ20_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBK2388133.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(6190..6720)
                     /gene="fimF"
                     /locus_tag="LGJ20_004131"
     CDS             complement(6190..6720)
                     /gene="fimF"
                     /locus_tag="LGJ20_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBK2388134.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6731..9367)
                     /gene="fimD"
                     /locus_tag="LGJ20_004132"
     CDS             complement(6731..9367)
                     /gene="fimD"
                     /locus_tag="LGJ20_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBK2388135.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLLS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSIIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(9434..10159)
                     /gene="fimC"
                     /locus_tag="LGJ20_004133"
     CDS             complement(9434..10159)
                     /gene="fimC"
                     /locus_tag="LGJ20_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBK2388136.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10196..10735)
                     /locus_tag="LGJ20_004134"
     CDS             complement(10196..10735)
                     /locus_tag="LGJ20_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2388137.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDQGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(10800..11348)
                     /gene="fimA"
                     /locus_tag="LGJ20_004135"
     CDS             complement(10800..11348)
                     /gene="fimA"
                     /locus_tag="LGJ20_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBK2388138.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
     gene            complement(11828..>11965)
                     /locus_tag="LGJ20_004136"
     CDS             complement(11828..>11965)
                     /locus_tag="LGJ20_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="inversion of on/off regulator of fimA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HBK2388139.1"
                     /translation="RLIQDYLGHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREE
                     V"
BASE COUNT         2866 a         3081 c         2920 g         3098 t
ORIGIN      
        1 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
       61 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
      121 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt cactgcgcca acaaagtcgg
      181 cattctgcgg cagatcatcg gcaaaaatac cgctcaggcc cagcagcgct ttcacgcggt
      241 ctgcgccctg atactgcgcg ttgatcttct ggaactccgc cagcatcggg tcgaccacgt
      301 caatggcatt accctgctca tccacgccct gggtgtaacg catccagcca gccacgccca
      361 gcgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccggg tccagcagac
      421 gctgcggtaa cttctggctg ccgtccatcg caatctgcca ggtacggtga cgcagagacg
      481 ggttgctgaa acgctcgatc agcaacgtcg cataggcgtt caggtccgta ccttccggca
      541 tcgacagcgt tggcgcttgt tcctgcatca tcagggagaa ggccgctttg cgataagccg
      601 ggttagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagaa
      661 aagagtggct gccgttcagc atacgcagct tcatcatttc gaacggcaca acgtctgcaa
      721 cgaactgtgc gcccacttta tcccagtccg ggcgaccatt aacgaagtta tcttcaatca
      781 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
      841 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
      901 ggaaggtgac attttcttca atccatgccg ccagctgcgg gtcacgcgcc tgagccagcc
      961 ccagtaccgc gaccttcgcc acatgaccgt tttcacgcac gttatcgcag gacatcaccg
     1021 taaacgcttt caaccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     1081 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     1141 gttgaccgct tgccgcatca gcgcagtagc ctttttccgt gaccgttaga gagacaatcg
     1201 ccgtttgcgg acgcgccatc gcgttgagaa taccttcgca gccatcgatt tccggatgca
     1261 gcgcttcttt catcgaaccg ataattttca gctcggtgct ctctgcgcct ttttccgcta
     1321 cggtgtacag cagttgctgt tttttcaggt tttcgatcag cacgcggtcg ttgcctggca
     1381 tcaggttaac ttcgcagatg ccccagtcgc tgtcggtgct ttccagcaga tgatgggtat
     1441 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     1501 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     1561 tcgtaaactc cagaatccgt cttcaaagag tgacggcaag gaagaaggaa ccggcacggc
     1621 gcgcagccat gccggtggat attaacggct aaagaaagcg cgctggatcg ccagttcgac
     1681 accgcgaact tcggccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     1741 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     1801 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     1861 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     1921 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     1981 atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg gtaaaaccgt ttgccatgct
     2041 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     2101 gaggatcggg cgcggcggat cgtccgggtg aacagccata cgcacgccga cttcttcagc
     2161 aactggaata atcgctttca ggaagacggc aaagttttcg cgcagcttcg ctttgtcgat
     2221 atctttgtac agctccaggt gtttacggaa ctggtcgagg gtgtagcctt cttccgcgcc
     2281 cggaagacca gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     2341 gaagcgaaca gcagcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     2401 tttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     2461 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     2521 gtagcacacg gtgcgaatgc cacactgcgc caggttgcgc agagtctgct gatagttagc
     2581 gatccactgc tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gtacgctttc
     2641 tacgacagac cacaccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     2701 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     2761 cgcctgacgg acatcagcta aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     2821 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgc ggaaccctgg
     2881 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     2941 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaattctgaa attggttaac
     3001 cacatcacaa gaatttcact ctcctggttg ttgctgcaaa ttgcagcaaa aggttatcga
     3061 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     3121 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     3181 attctctggg tagtattcgg tatcggcctg atgctggtac tgaatttgaa gttcaaaatc
     3241 aattcaatgg tggctttgtt ggtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     3301 ttgatgtcgc tgctgcacac tatgaaagcg ggcttcggca acacgctggg ggaactggct
     3361 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggctcac
     3421 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtgatt
     3481 atcatcggcc tgatttttgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     3541 ccgctggtta ttgttattgc cgccgaagct aaaattccgt tcctgaaact ggcgatcccg
     3601 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     3661 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     3721 atcccaagtg taatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     3781 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     3841 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     3901 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     3961 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tctttggcac cgcacgtggt
     4021 catgacatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagtat tgcaatggtg
     4081 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggc
     4141 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     4201 atcactgtgc taattcgtct ggcgacgggt cagggtgtcg tttcggcgat gaccgccgcc
     4261 gggattatca gtgctgcaat cctagatcca gcaactggtc agctggttgg cgtgaatccg
     4321 gcgctgctgg tactggcgac ggctgcgggt tccaacaccc tcacccacat taacgatgca
     4381 tctttctggt tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     4441 ggtctgctgg agctggtcaa ctccgtggtt gggctgatta ttgtgctgat tattagcatg
     4501 gtagcgtaaa aggacaccag agcctgccaa tggcaggctc tgactgatga caaagtctaa
     4561 aaatgtgccc agacagtcct ctcgcccctc tggggagagg gttagggtga ggggaaaaga
     4621 ccagcaaagt gcgctttgtt tatgccagat gcgacgctaa cgcgtcttat ctggcctaca
     4681 aagggctaac gtgcaggttt ttagcttcag gtaatattgc gtacctgcat tagcaatgcc
     4741 ctgtgatttc tttattgata aacaaaagtc acgccaataa tcgattgcac attccctgca
     4801 gtcacctgcc ctccggtacg tgcgtaattt gccgttaatc ccagacttac cgccgaagtc
     4861 cctactgctc ctaacgatac cgtgttattc gctggaataa tcgtaccgtt gcgcgtcaac
     4921 tgtacgccga cgccctgcgc tggtgaaaac gacgcggtat tggtgaaaat cgagttgccc
     4981 gcatctgcgg ttgtgccgga gaggtaatac cccaggtttt ggcttttcgc acaataaacg
     5041 gtaagaggaa ttggcactga accagggtag tccggcagag taacggtgac atcacgagca
     5101 gaaacatcgc agccgccagt aggcaccacc acatcattat tggcgtaaat attccacaca
     5161 aactggaaat catcgctgtt atagttgttg gtctgtcgca aaataagcac ggcaattaat
     5221 gagccagctt taatcgccac cccgcccgca ctgctcacag gcgtcaaata aagcgccacc
     5281 ggccacggct tatccgttct cgaattataa acaacccgcg gcgtttcgct ggtggtcgga
     5341 aatggatagc tactgccact atattttacg gtcccggaaa aattagataa cacgccgcca
     5401 taagccgagc ctcgttgcag tgtgacatag tctgtaatgg tttccggata atcgttatgg
     5461 caaaagattt gcgtcgaaag atctacgacc aggttttgcc ccacattcac ggcaggcgca
     5521 aggtttacat aaacattagc gctgccaccg ccaataggga tagctgtacc attggcggtt
     5581 ttacaggcga atgaccaggc atttaccgac cagcccatca gcagtacagc aaacagggta
     5641 ataactcgtt tcattacaat catctcttcg ggttcagctg taggtatagg tgatgctaat
     5701 cactgcctga atggttccct gagtggctcc gccatttact gtcaatgctc tgacctgtaa
     5761 cgggaagtgc gctgattgtg aggaatcatc cacctgaact gttttggttg cgccagtatt
     5821 caatgtgttg ccactgtcat cctgtagctc taactggatg ttttgcgcgg tcccctggtt
     5881 tttataatat ccggtactgt cggctgcccc gctgaagctg gcagtgaccc tcgacgttcc
     5941 caccggacaa ttagtcaact caagcgcaac atcatgccag gccgatgccg ccccggcaga
     6001 catcagactg aaagaataaa gatcgccgag atcaaccgtg gcattggtgg tggaaaccgt
     6061 gcacggtttg gcgacgacct taccgttcac cgtgatggtg acatcggctg cctgtatcgt
     6121 cgcacttgcg agcgccaata ttgccgccaa taaataccca cgtttgcgcc atttcatgag
     6181 catctccagt tactgatatt caagagtgaa ggtagccgtg gcattgatat gccccgcagt
     6241 gacaggcacc tgtgtcgcca ttagccgggc gtaaaaattc aacgtatttg gtttacccgg
     6301 cgtcagggtc gtccacgaaa tcgcggacga tggagcatta aggggtatct gattttgctg
     6361 ctcattcaga agctgtattc ccagtcccga agccgctgat accgtatttt caagtgcaag
     6421 caggttggca ttgtggctat ctgcaacgcc ggtaaaccca acctttacgg cagaaacggc
     6481 gttaccacag ggtgacagca aaatacgaaa tggaacgaca ggagtcgttg cgccaatgtt
     6541 gttaaattgc ttcgccgcgt tttccatcag atcaacagta aaattggttg attcagcggc
     6601 cacactacag ccgttatctc tgacatagcc gcggatagta atcgtgctat ccgcagccaa
     6661 aacgcgactt accgccagcc acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat
     6721 cacgccctcc ttaacgacat tcagctgata gctgggttaa taactgctgc tgactctctg
     6781 gtggcagttg ataattggcg atacagtgag cattttcctc ttctccccat ttcacctgaa
     6841 cttttcccgc taaaggcatt ccgctgaggt aaacctgacc attatccgca acaatgccgc
     6901 tactctggct actctctgat gtcaccatcg ccccaaacgg cagcggctta ttattgtggg
     6961 ttagcgtcat gagcagtttt atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc
     7021 cacgagtggg aacaacgtta gcgaccgcgt tatctaaatc gacgttatca gccagggtat
     7081 tggtatccag cgccactcta ttttcccgat attcagtggc ataaggcagc acggcataac
     7141 cacgccagtc ggtacgcacc cccgtctggt tttcgacttt tgcatctttt gcgccaggcg
     7201 ctttaacaag caccaccgta tcgtttaacg gctgccccag cgttacgcca ttggcatgag
     7261 ccagtacccc accgctgact ccgtaataga gctgcttaat atcatcacta tggctgtaac
     7321 cgatattggc attgccgtaa ccaccgcgat aattcagcgt ggcgtagcct gtgcttccgc
     7381 tattaccatc gcctcccccg gcatagccgg tttgcacgct atagctgagg ttgttgtctt
     7441 ccagcaacgt accgtataca ccagccagat tggtcattcg accgttgaga tcgtgtgaca
     7501 tgctgtagct ggcactggca tgtcgccact gagatttact gtcagaacgc agccagtggc
     7561 tgaaaggaat attgacgtta agcgctaaca tctgatcacg tcctttttgc caggcgtttt
     7621 tcgtcaggct atagctgagc gtccagttga tatcttcgaa cgcagtattt aatccagcct
     7681 ggaattgctc atcgacatta ctcgttcccc aataagtttg atggctacca ctcaaataca
     7741 gtgttgatga gcgcccgagt tgctgagtaa cggtgagttg taatttcccg cgtttgttat
     7801 aagcgaggtt gtaatagtcg gtgaatttcg gcttaacctg aataactccg tcctgtgttt
     7861 cgatgttgta gccattcatt cgactgtatg ttgtatcagc gaaattaaaa tatccgctgg
     7921 tcgaataacg gtaacccact aactgaatat tcgtgcctga ctcattgagc gatttgttat
     7981 agagaaaacg caccgattgt ccgtcatgct gactgtcatc gggaagtgtg gaattagcct
     8041 gcgtcatatc cacagacaga gcgcccagtg cccccatgtt tttcccgata ccgaaattaa
     8101 aagcacgata acgatccgcc agttgcgttc caccatatat tgtccagcca gccggaaggc
     8161 cgtggagtaa tgtactctgg aaaaagcggg gtttttcctg ctgcgcattt ccactacggt
     8221 attctcctgc cgtaatggaa taacgagtat gcccttcacg ttgcaaaagc gggactgacg
     8281 aatagggtac ggtaaaaatc tgcgtgctgc cgtcagcctc tttgatcgtt acctgcaagt
     8341 caccactatt acctgcggca tagatatcgt tgatggtaaa aggccccggt ggcaccgtac
     8401 tattataaat gtcataccca ttttgtttaa tagtgacctg tgcagtacca cgagcaatac
     8461 cgtggatcac cggggcaaat cctctttggc tatcgggtaa catattgtca tctgaggcca
     8521 attgtgcgcc gcgaaagtta ataccatcga aaatatcgcc ctgagtataa ccatcaccca
     8581 gcgtcagccg ggaacgtaac ggtattatgt ctcgctcaag ccaggtattg atatgctgcc
     8641 atttattttt gctacctgat gatctgtcgc tactgttata actccaggtg gtattgtcgc
     8701 gtaaacgcca cgcaccaata tttaacccac tctgtaggtt taaatatgca taatggctgt
     8761 tacccccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aatccggcat
     8821 taataccggg atcccataac tcaggaggaa tataaccacg cgcgcgatta ctcataaatg
     8881 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
     8941 ggattattga ggttaatggc acgcaggcat catccgccag cagattcata ccggagacag
     9001 aagccgtatt cagccccata ctggcaagtt gcgcgcgtgt caggcaggga acaatccctt
     9061 gttcgctgtc gcccgtatta aatgtgacat cacgcgttgc catataacca ttattcaaat
     9121 agatatcgac acgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
     9181 cagccacagc ctggggatca tccgctaaaa agcgcgggtt aaaatagagt tcggcagata
     9241 acaaaggtgc ctgtgcggca aaagcacagg caacaacgag ccggacaaaa aaaccagcca
     9301 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
     9361 atgacattat tcctccggtt taaaatcacc cggcagtcaa ttctttttat tcaggcgaaa
     9421 aattccccct gcgttattcc attacgcccg tcattttggg ggtaagtgcg ccataatcat
     9481 ttattgttcg gtaagtaata ttgcttcccg catcagaagg caatttaacc gtgctttcgc
     9541 ccattggagg caccaaggca ttttcaagaa cccgggctcc ggcattcaac tctgttaccg
     9601 tcaggtaata gggtgtcggg ttaatcagcg tcagagaatt cgcgctacga cgaaatctta
     9661 atttttctgc ggcctgatcg ggtggcaacg ctaatttagc cgggcgatag tacagtttaa
     9721 tgcggctgat aattgcgagc tgtagcgtat tctcagtcaa ttttgattta tccattgacg
     9781 gaatcgcttt aacattcatc cagaataaac tttcccgatc ctgtggcaat tggttatttg
     9841 ttgcatcaag aatacgtaag gtattctctt tttttccctt catcgcaaac agaggaggcg
     9901 tcacgataaa acgaccatcc tttacaccat cggcattttc cacccatgat tgaattaaat
     9961 aggtactatt ttcatcatta tttgtcacgg caagttgcac ttgtttttgc cctgccggat
    10021 aaattacgcg agtcgcacct aaggccactc ctgcttcagc gcgtccggca accatcattg
    10081 ccatgaacat caggatacct gccagcaagc agaatgttat ttcctgcgat ttccttacat
    10141 tgacgttttt attactcact gtcctgttcc tgttatcaca ttatctgctg aacaattact
    10201 gataggttaa agagaaccag gcctgggcat tggcgatgcc gccagtaacc cgacgcccgg
    10261 tagcacgata tttggcgatg aaatgtagcg aagtagagcc tgagtaaagc cgtttccagt
    10321 ttgctggagg acgattaatc ggtacgaggt ttccttgatc atcaaacaac gctacgccaa
    10381 tattggtggc tatccctggc ccctctccca cggaaagcac atccggattt ttaccatccg
    10441 cgacaccgtg aaacgccaca cctacacgtt cactcaccac cgtgctacat tcccgtaaat
    10501 gaataacaaa aggcaccggt gcgctatctt cccctaccgc atgaaaccgg ttactgctga
    10561 tttgccccat attgaccgtc atttgtttat caccggcttc aatccggcaa gtttccgcaa
    10621 taatgacgcc ctgaaattgc atatttccgc cgggcagcgt ggtattccat ttatttccgg
    10681 ccagagcaaa cattggcagc aacgacgcta atagaaataa tctcttcctt ttcatcgtta
    10741 tttttatcgc acaagggtgg gcatccctgc ccgtaatgac gtccctgaac ctgggtaggt
    10801 tattgatact gaaccttgaa ggtcgcatcc gcattagctg cgccaggggt tgcggcaccg
    10861 gttgcaaaat aacgcgcctg gaacggaatg gtgttggttc cgttattcag ggttgtttct
    10921 gaactaaatg tcgcaccgtc cagcgccagc gcagcacccg ttctgtccag gatctgcaca
    10981 ccaacgtttg ttgcgctacc cgcagctgaa ctctgcagag ccagtacatc agtatgcgta
    11041 gcatcaattg ccgtacctaa gaaggcaaca gcggcttttg tggcaacagt ggtatcgcaa
    11101 tcattcagct gaatgttaaa accaacggca gagctggttg ctccagcctg cttcaggcta
    11161 gcggtacgaa cctggcccaa ctgaacggtt tgatcaacag agcctgcatc aactgcgcaa
    11221 gcggcgttaa caacttcccc tttaaagtga acggtcccac catttaccgt cgtagtatcg
    11281 gccagagccg ctgcggaact gagggacaga gccgacagaa caacgattgc cagagtttta
    11341 attttcatgc tgctttcctt taaaaaacta tttctaaatc gacatgggca gtcgttctgt
    11401 acactttgtt ttgtcaacga gtttgactgc caacactgca cagttttccc ccaaaagatg
    11461 aaacatttgg ggccattttg actcatagag gaaagcatcg cgcacaaact ttttcagttt
    11521 atttgttggc ttaatattct attgttatct ttatttatag atttttatat tgcatgaggt
    11581 ggtttttgga gagaagaatg aggaagatgc gtcgagccac agaaacgtta gctttacata
    11641 tagcggaggt gatgtgaaat taatttacaa gagaaataat ttacatatca aacagttaga
    11701 tgctttttgt cgttttttaa tatttttatg cttgagaaaa aatacgtaac ttatttatga
    11761 tatggacagt ttggccccaa ttgtcttgta tttatttgtt ttttaacttt attatcaata
    11821 agttaaatca aacctcttct ctttttaatt tttcgtttat gagattattt ctttcccata
    11881 gtccggcaaa acgagcagca ttactggcgg tataacgcac agtatggcga atatttcgat
    11941 gcccgagata atcctgaatt aaacg
//
LOCUS       DAFATA010000125        11948 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFATA010000125 DAFATA010000000
VERSION     DAFATA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.126"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1184
                     /locus_tag="LGJ20_004137"
     CDS             <1..1184
                     /locus_tag="LGJ20_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBK2388140.1"
                     /translation="DQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTID
                     LSGLNLTTQPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLDSINFPQGRNV
                     SITHISMNNNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIV
                     AGNKNELLLLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKISYSNFCNVQTLNAAN
                     NQLKYINLDFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSG
                     KNNCPTMQLMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPY
                     NIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWP
                     F"
     gene            1508..2896
                     /gene="narU"
                     /locus_tag="LGJ20_004138"
     CDS             1508..2896
                     /gene="narU"
                     /locus_tag="LGJ20_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBK2388141.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2978..6718
                     /gene="narZ"
                     /locus_tag="LGJ20_004139"
     CDS             2978..6718
                     /gene="narZ"
                     /locus_tag="LGJ20_004139"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBK2388142.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGAI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIESIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6715..8259
                     /gene="narH"
                     /locus_tag="LGJ20_004140"
     CDS             6715..8259
                     /gene="narH"
                     /locus_tag="LGJ20_004140"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBK2388143.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            8259..8954
                     /gene="narW"
                     /locus_tag="LGJ20_004141"
     CDS             8259..8954
                     /gene="narW"
                     /locus_tag="LGJ20_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBK2388144.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8951..9631
                     /gene="narI"
                     /locus_tag="LGJ20_004142"
     CDS             8951..9631
                     /gene="narI"
                     /locus_tag="LGJ20_004142"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBK2388145.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9710..10603
                     /locus_tag="LGJ20_004143"
     CDS             9710..10603
                     /locus_tag="LGJ20_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBK2388146.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(10699..11544)
                     /gene="nhoA"
                     /locus_tag="LGJ20_004144"
     CDS             complement(10699..11544)
                     /gene="nhoA"
                     /locus_tag="LGJ20_004144"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBK2388147.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11717..>11948
                     /locus_tag="LGJ20_004145"
     CDS             11717..>11948
                     /locus_tag="LGJ20_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBK2388148.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIP"
BASE COUNT         2964 a         3066 c         2997 g         2921 t
ORIGIN      
        1 ttgatcaact aatatcatca aaacatcttt ggcatgtatt agttaacggc atcaacaatg
       61 atccacttgc acaaatgcag tactggactg cagtaagaaa tataattgat gacactaatg
      121 aagtgaccat tgatttatca ggacttaatt taaccactca accaccaggg ctgcaaaact
      181 tcacctctat caatcttgat aataaccaac tcacacattt tgatgcaacc aactacgata
      241 gactcgtaaa gctaagtctg aatagtaata ctcttgactc aataaatttt cctcaaggca
      301 gaaatgtaag tattacacat atatctatga ataataatgc tctcagaaat attgatatag
      361 ataggctttc atcagttact tattttagtg cggcacataa tcaactagag tttgtgcaat
      421 tagaatcttg cgaatggctg caatacctga atctcagcca taatcaatta actgatattg
      481 ttgcaggtaa taaaaatgaa ctcttactgc tggatctatc ccataataaa ctaacaagtt
      541 tacacaatga cttatttccc aacttgaata cgttacttat taacaacaat ttgctttctg
      601 aaattaaaat atcctatagc aacttctgca atgttcagac attaaacgct gctaacaacc
      661 agttgaaata tataaatctt gatttcctga cttatcttcc atctatcaaa agtttaagac
      721 tggacaataa taaaataacc cacattgata ctaataatac atccgatatt ggaactttat
      781 tccccataat aaaacagagc aaaaacttaa attttttaaa tgtttctggg aagaacaatt
      841 gccctactat gcagctcatg ttatttaatt tgttttcccc agcacttaag cttaatactg
      901 gcctggcaat tctttcgcct ggtgcatttg aagttcactc tgacggaata gatgtggata
      961 acgaattgtt tcactatcct attaaaaaag catatacccc atataatata cacacttaca
     1021 agacagagga agttgtaaac cagaggaata taaaagttaa aaacatgacc ttagatgaaa
     1081 taaacaatac ttactgtaat aacgattatt acaatcaggc aataagagag gaaccgatag
     1141 accttctgga cagatcgttt tcctccagtt catggccttt ttagtgaata aatcatcact
     1201 aattctataa gtgtctggtc ttgttgataa tatagttaat atgtaaccct ttagattcac
     1261 gatctgaagg gttgaaacat gacatacgtt caaagattaa cccccgtttg aaggttagct
     1321 tgttagttac atttagtaac acatatctgg ctacttaatc cccattctgc ccccgcggtt
     1381 acgctttctt atctattctt tcaatcagca cttcacccaa ttcatcctta attctaataa
     1441 ctggctattg cgccttacct cctgttttgt cttttttcat taccaatgtg tgcatgtgag
     1501 gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg actggaaacc
     1561 agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa acctctggat
     1621 atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg cagttaccgt
     1681 taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat taaccgcatt
     1741 accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc ctatattcgg
     1801 tggacgccga tggacggttt ttagtactgc aatcctgatt attccttgcg tctggctcgg
     1861 aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg ctttgctatg
     1921 cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct tctttccaaa
     1981 agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag gtgtaagtgt
     2041 aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc tcggcgtcaa
     2101 tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat ggatttgggt
     2161 accgctactg gcgattgcca cgatcgccgc ctggtcaggg atgaatgata tcgccagttc
     2221 acgcgcctca attgccgacc agctccctgt cttacaacgc ctgcatctct ggctgctgag
     2281 cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt ttgccatgct
     2341 ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg gcccatttat
     2401 cggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aagttcggcg gcgtgcgggt
     2461 gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc ttaccttacc
     2521 gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc tgtttctgac
     2581 tgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc gccagataac
     2641 catttatcgg gtaaagatga aaggcggtag tgatgagcaa gctcagaaag aagccgtcac
     2701 cgaaacggcg gcggctctgg gctttatctc agccattggc gcagtgggcg gcttttttat
     2761 tccgcaggcg tttggcatgt cgctcaatat gaccggctct ccggtcggcg cgatgaaagt
     2821 gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg gtcggcggaa
     2881 gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc cgacaaggca
     2941 tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt tggatcgctt
     3001 tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg tgatgcatag
     3061 caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa tcgtgcgttc
     3121 cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta aaaatggtct
     3181 ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc tgcccaatca
     3241 tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctttaca gcgctaaccg
     3301 cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgcgaag ccctcaagca
     3361 acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa agtgcctgag
     3421 ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg aactaaacca
     3481 gctgattgcc gccgctaacg tctggaccat caaaacctac ggcccggatc gcgttgccgg
     3541 tttctcgccg atcccggcga tgtcgatggt ttcttacgcc gccggaacgc gttatctgtc
     3601 actgcttggc ggcacctgtt taagcttcta cgactggtat tgcgacctgc cgcccgcctc
     3661 gccgatgacc tggggcgagc aaaccgacgt accggaatct gccgactggt ataactccag
     3721 ctacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg cccacttctt
     3781 taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact actctgaagt
     3841 ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg ccctggcgat
     3901 ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg actactttat
     3961 caactactgc cgccgctaca gcgacatgcc gatgctggta atgctggagc ctcgcgacga
     4021 tggtagctac gttcccgggc gcatgatccg cgcatctgac ctggtggatg gactgggcga
     4081 aagcaacaat ccgcagtgga aaaccgtagc agttaatacc gcaggtgaat tggtagtgcc
     4141 gaacggtgcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg aatccattgc
     4201 cgccggtacg gaaaccgaat tgtcgttaac cctgctcggt caacatgacg ctgttgcagg
     4261 cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg taaaacacaa
     4321 tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg gcaacacctg
     4381 tccggtggtc agcgtttatg atttggtact ggcgaattac ggcctcgatc gcgggctgga
     4441 agatgaaaac agtgcgaaag attacgctga aatcaaaccg tacaccccag cctggggtga
     4501 gcaaattacc ggcgtgccgc gccagtatat tgaatccatc gctcgtgaat ttgccgatac
     4561 tgcccataaa acgcatgggc gctcaatgat tatcctcggc gctggcgtca accactggta
     4621 tcacatggat atgaactacc gtgggatgat caatatgctg atcttctgcg gttgtgtcgg
     4681 gcaaagcggt ggcggctggg cacactatgt cggccaggaa aaactgcgcc cacaaaccgg
     4741 ctggttgcca ctggcctttg cgctcgactg gaaccgacca ccgcgccaaa tgaacagcac
     4801 ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg ctcaggagtt
     4861 actttcaccg ctcgccgatg ccagtaagta cagcggtcat ctgattgatt tcaacgttcg
     4921 cgccgaacgt atgggctggc taccttctgc gccgcagctg gggcgtaacc cgctcgggat
     4981 taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc aggcgctgaa
     5041 atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc atccgcgtaa
     5101 tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg agtatatgca
     5161 gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg ccagcgacgg
     5221 gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct
     5281 ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg ttctgcccac
     5341 cgccacctgg tacgaaaaag acgatatgaa cacctcggat atgcatccgt ttattcatcc
     5401 gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa tctacaaagg
     5461 tatcgccaaa gcattttcgc aagtgtgcgt gggtcatctt ggcaaagaaa ccgacgtggt
     5521 attacaaccc ctactgcatg actctccggc agagctctca cagccgtgtg aagtgctcga
     5581 ctggcgcaaa ggcgaatgcg atctgattcc aggcaaaacc gcgccgaata ttgtggcggt
     5641 ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat tgatggacaa
     5701 acttggcaat ggcggtaaag ggatttcgtg gaatacccag gatgaaatcg atttcctcgg
     5761 caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga ttgacaccgc
     5821 cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc atgttgcagt
     5881 caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc tggcgctgca
     5941 caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta aaattatctc
     6001 cagccccacc tggtccggtc tggaaagcga tcacgtatcc tataacgcgg gatacaccaa
     6061 cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct atcaggatca
     6121 tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta tcgacacccg
     6181 tagcgtcagt gagatgcgcc agataccgcc aaacggcttc ccggaaaaag cacttaactt
     6241 cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc tgctaatgct
     6301 gacgctctct cgcggtggac cgattgtctg gatcagcgaa acagatgccc gagaactgac
     6361 cattgtcgat aacgactggg tggaagtatt caacgccaac ggcgcgctga ctgcccgcgc
     6421 ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc aggaacgcat
     6481 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata actcggtcac
     6541 ccgcgtttgc ccgaaaccaa cgcatatgat tggcggttac gcgcagctgg cctggggctt
     6601 taactactac ggcaccgtcg gatcgaaccg cgatgagttc atcatgatcc gcaagatgaa
     6661 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
     6721 atccgttcac aagtcggcat ggtgcttaac ctcgacaaat gtatcggctg ccatacctgt
     6781 tcggtgacct gtaaaaacgt ctggaccggg cgcgaaggca tggagtacgc atggtttaac
     6841 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
     6901 caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg cagcaagatg
     6961 ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga ttactacgaa
     7021 cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca tatccctact
     7081 gctcgcccgc gttcactgat tgacggcaaa cggatggaca aagtgatctg gggcccaaac
     7141 tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa cttcgaggcc
     7201 atgcaaaagg agatgtacgg gcagtttgaa aacaccttca tgatgtacct gccgcgcctg
     7261 tgcgaacact gcctcaatcc cagttgcgtg gcgacctgcc caagcggcgc tatctacaaa
     7321 cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg gcgtttgtgc
     7381 attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa gtcagaaaaa
     7441 tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc agaaacctgc
     7501 gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat tgaggaagcg
     7561 gcgagcaccg agcgcgaagt tgacctctat gaacgtcagt gcgaagtatt cctcgatcca
     7621 cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa cgtgattgaa
     7681 gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc gctaccgctg
     7741 caccctgaat atcgcaccct gccaatggtc tggtacgttc ctccgctgtc gccgattcag
     7801 tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc catcgaaagc
     7861 ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac cggtccggta
     7921 ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca aaccgtggaa
     7981 ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca ggtcgaagag
     8041 atgtatcgtt acctcgccat tgccaactat gaagatcgtt ttgtcatccc gacgagccat
     8101 cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac ctttggcgac
     8161 ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat cgatgccatc
     8221 aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc aaagtgatcg
     8281 gcctgttgat ggagtatccg gacgagctgt tgtgggaatg caaggaggac gcgctggcgt
     8341 tgatccgcag cgacgcgccg atgcttacgg atttcaccca caacctgctt aacgcgccgc
     8401 tgctggataa acaggccgaa tggtgcgaag tgtttgaccg tggacgtacc acatcgctgc
     8461 tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg gtggacctgc
     8521 tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc gattatctac
     8581 cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa ggattgctca
     8641 atgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag gcaccgtggt
     8701 acgcgttgtt tgatgctctg ctgcaactgg cgggaagctc cctttcaagt gacagtgtca
     8761 caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat gcagtgtggg
     8821 aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc tcgccgctta
     8881 atcaatatca gcgacgcttt agccaggatg tcgcgccgca atatgtcgac atcagtgcgg
     8941 gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc cgtacatttg
     9001 tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt acacctggcg
     9061 cgcctcctca agtcaaatgc tcgataaacg cgggatggtg atatggtcga atctgttcca
     9121 tatcggcatt ttggggattt tcttcgggca cttgttcggc atgttaacgc cgcactggat
     9181 gtacgcgtgg tttttgccag tggcagcgaa acaactaatg gcgatggtgc tcggcggtat
     9241 ttgcggtgtg ttgacattga ttggcggcgc aggactgctg tggcgcaggc tgacaaatca
     9301 gcgcgtgcgt gcaacttcca ctacgccgga tattatcatc atgagcattt tgctgatcca
     9361 gtgtctgctg gggctaagta ccataccgtt ttcagcacag tatcctgatg gtagcgaaat
     9421 gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat cgtcagaaat
     9481 gcttaacggc gtagcgttcg ttttccgcct gcatctggta ttgggaatga ccatcttcct
     9541 gctcttcccg ttcaccagat tggtgcatgt gtggagcgcg ccgtttgagt actttactcg
     9601 tcggtatcag attgtgcgtt cgcggcgata atttcaccct ggccggatag cccttcgctc
     9661 tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa tgaaaccgca
     9721 ggtgtaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg ccggtgttgt
     9781 tttccccgcc gataatctta gcgaagcgca aatgcagctt atcgcccgcg agttaggcca
     9841 ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc gctactttac
     9901 gccaacggtt gaagtgccga tttgcggtca cgcgacagta gctgcgcact atgtacgtgc
     9961 gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca tcgctggcag gaaaacatcg
    10021 cgtgactatc gaaaagcaca acgatgatta tcgtatttcg ctggaacaag gtacgccggg
    10081 ctttgaaccg ccgctggagg gtgaaacacg tgaggcgatt atcaacgcgc ttcatctcac
    10141 tgaggacgat attctgccag gcttgccaat tcaggtggca acaacaggac actcaaaagt
    10201 gatgatcccg ttgaaaccag aagtggatat cgacgccctt tcgcctgatc ttgctgcgct
    10261 gagcgctatc agcaaacaga ttggctgcaa tggtttcttc ccgttccaga tccgcccagg
    10321 caaaaacgaa accgatggtc gcatgttctc gcctgcgatt ggtattgtgg aagatccggt
    10381 gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg tattgcccca
    10441 cgatggcaag gtgctgcgtg ttaaaggcca tcaggggcgc gcattggggc gtgacggcgt
    10501 gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca tttcaggtac
    10561 agcggtgatt ttgtttcatg ccgaatgggc aattgaaatc tgaaatcgtt gcctgatgcg
    10621 cttcgcctgt caggtctaca accgggctaa tgtatgccgg ataaggcgtt tacgccgcat
    10681 ccggcaacct gacataaatt attttcccgc ctccggatgc gtatcaaacg ccgccatcac
    10741 cagcgctaac tcatccacag taaagccatg tttcgcatca tccacgccca gaccaaactg
    10801 ttcctgcatc acggcatata acgacaccac atccggtaga tttcgctgct ccaccgcgtg
    10861 cccattttca taatgggtaa aatgaaaatt ggtcagcgtt agcttgccac cgtccggcaa
    10921 atggcggcac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg ccgaccagaa
    10981 attgcccatc acataatcgc tttgttgctg ctcgcagaga tcaaaacggt acatcgactg
    11041 ccaatgctga tgatgattaa actgcaacac ccaatcatca ccctcctgca acaaccgata
    11101 ctctccgtgt ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg cggttagcgt
    11161 ctgcccaccg aaaccgacat cagcaatcca tttttcctct tccagttcca ccaacagcaa
    11221 acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgcc ctaacaagct
    11281 gcgaacgtta aactccagct cgcgtaacac ccgctcaaac acgccattct gctcaaaaca
    11341 gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat caagctgtat
    11401 ttccctcggc agcaaaacgt cgaggttttc aaacggaata gtgcaattgt gtttcaggtg
    11461 caatgcacga agcgtatcaa tattgaccgc agcagctccc gaccagttaa tacgggcaaa
    11521 atagtgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc aacttataac
    11581 ctggaataga cgccgcaccg gctattttgt gcgatcgcgg acatgcatca cgtttgcaaa
    11641 tcgccaatcc cgttaatttc gttacttatt gcgttacgga ctacgcttaa aaaaaccttc
    11701 ttcgcgaggc gataccatga gccgattcat ccccatagaa ttacaccatg ccagccgtct
    11761 gttaaatcat ggcccaactg tcatgatcac cagtttcgat gagcaatccc agcggcgtaa
    11821 cattatggca gcagcctggt caatgccggt ggagtttgaa ccgccacgcg tggcgattgt
    11881 ggtagataaa tcgacatgga ccagggagtt gattgaacgt aacggtaaat ttggcatcgt
    11941 tatcccgg
//
LOCUS       DAFATA010000126        11552 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFATA010000126 DAFATA010000000
VERSION     DAFATA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.127"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..360
                     /gene="pcnB"
                     /locus_tag="LGJ20_004146"
     CDS             <1..360
                     /gene="pcnB"
                     /locus_tag="LGJ20_004146"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Polymerase that creates the 3' poly(A) tail found
                     in some mRNA's; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HBK2388149.1"
                     /translation="LAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWK
                     LLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPR
                     RRTRRPRKRAPRREGTA"
     gene            357..836
                     /gene="folK"
                     /locus_tag="LGJ20_004147"
     CDS             357..836
                     /gene="folK"
                     /locus_tag="LGJ20_004147"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBK2388150.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            1206..1790
                     /locus_tag="LGJ20_004148"
     CDS             1206..1790
                     /locus_tag="LGJ20_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388151.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            1895..2635
                     /locus_tag="LGJ20_004149"
     CDS             1895..2635
                     /locus_tag="LGJ20_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBK2388152.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            2670..5270
                     /gene="htrE"
                     /locus_tag="LGJ20_004150"
     CDS             2670..5270
                     /gene="htrE"
                     /locus_tag="LGJ20_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HBK2388153.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RLSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            5287..5856
                     /locus_tag="LGJ20_004151"
     CDS             5287..5856
                     /locus_tag="LGJ20_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388154.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            5871..6473
                     /gene="yadL"
                     /locus_tag="LGJ20_004152"
     CDS             5871..6473
                     /gene="yadL"
                     /locus_tag="LGJ20_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBK2388155.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            6500..7096
                     /locus_tag="LGJ20_004153"
     CDS             6500..7096
                     /locus_tag="LGJ20_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388156.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            7149..8423
                     /locus_tag="LGJ20_004154"
     CDS             7149..8423
                     /locus_tag="LGJ20_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBK2388157.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            8536..9330
                     /gene="panB"
                     /locus_tag="LGJ20_004155"
     CDS             8536..9330
                     /gene="panB"
                     /locus_tag="LGJ20_004155"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBK2388158.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            9342..10193
                     /gene="panC"
                     /locus_tag="LGJ20_004156"
     CDS             9342..10193
                     /gene="panC"
                     /locus_tag="LGJ20_004156"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBK2388159.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(10267..11169)
                     /gene="rpnC"
                     /locus_tag="LGJ20_004157"
     CDS             complement(10267..11169)
                     /gene="rpnC"
                     /locus_tag="LGJ20_004157"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBK2388160.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            complement(11281..11493)
                     /locus_tag="LGJ20_004158"
     CDS             complement(11281..11493)
                     /locus_tag="LGJ20_004158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388161.1"
                     /translation="MSHFHAVEFALQHRANHNFYLSTLSLTKQAVPALRKFSRSIARF
                     LMSVYSSDGICVSSLRTAPKRAMYRL"
     gene            11443..>11552
                     /locus_tag="LGJ20_004159"
     CDS             11443..>11552
                     /locus_tag="LGJ20_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBK2388162.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAG"
BASE COUNT         3240 a         2578 c         2637 g         3097 t
ORIGIN      
        1 ctggcgatga acgacgtgct ggacgaagcc tgccgttcac tggcaatccc gaaacgtctg
       61 acgacgttaa cccgcgatat ctggcagttg cagttgcgta tgtcccgtcg tcagggtaaa
      121 cgcgcatgga aactgctgga gcatcctaag ttccgtgcgg cttatgacct gttggccttg
      181 cgagctgaag ttgaacgtaa tgctgaactg cagcgtctgg tgaaatggtg gggtgagttc
      241 caggtttccg caccaccaga tcaaaaaggg atgcttaacg agctggatga agaaccgtca
      301 ccgcgtcgtc gtactcgtcg tccacgcaaa cgcgcaccgc gtcgtgaggg taccgcatga
      361 cagtggcgta tattgccata ggcagcaatc tggcctctcc gctggagcag gtcaatgctg
      421 ccctgaaagc attaggcgat atccctgaaa gccacattct tgccgtttct tcgttttacc
      481 gcaccccacc gctggggccg caagatcaac ccgattacct caacgccgcc gtggcgcttg
      541 aaacttcgct cgccccggaa gagttactca accatacaca gcgcatagaa ctgcaacagg
      601 gtcgtgttcg taaagctgaa cgctggggac cacgcacgct ggatctcgat atcatgctgt
      661 ttggtaatga agtgataaat accgaacgcc tgaccgttcc gcactacgat atgaagaatc
      721 gtggatttat gctgtggccg ctgtttgaga ttgcaccgga gctggcgttt cctgatgggg
      781 agacgttgcg tgaagtctta catacaagag catttgacaa attaagcaaa tggtaattta
      841 tttatattac gaatataaat attttctgaa aatcatacat cctctctttc cttaataaaa
      901 attttagttt aaacattttt gcagaaacat aatataaatt cccgcagata aatatcaatc
      961 ccatcgccat accctaacca attgcacttg tgaatttatt tttgcttaat tttgaattaa
     1021 gaaaatttaa tttgttcaaa agcccttaaa gtgcacaata ggcgtcctaa tgaacaatgg
     1081 ttgtaaattt tggtaacaaa tatcattgct cattaatatc tagcatctgc ttataacatt
     1141 tgtgtcatga atagtcattc ttcaaaccag tagtctgact aatggaattt tattcaagga
     1201 tttttatgtc taaaaaatta ggttttgccc tgtctggtct tatgctggca atggttgcag
     1261 gtactgcttc tgctgatatg gatggtggtc agttaaatat cagtggtctg gttgttgata
     1321 acacctgtga aacccgcgtt gacggcggta ataaagatgg tctgatcctg ctgcaaactg
     1381 caactgttgg tgaaatcgat gctggcgtgc tgaatgacac cgttggcgct aaagccaaac
     1441 ctttcagcat caccgttgac tgcagcaaag caaatccgaa tccaggtagt acagctaaaa
     1501 tgaccttcgg ttctgttttc tttggtaata gcaaaggaac gctgaacaac gacatgtcta
     1561 ttaacaaccc gtctgacggc gtgaatatcg ctctgcacaa tattgatggt tccactatca
     1621 aacaggttca aattaacaac cctggcgatg tgtatactaa agccctggat gcaacgacaa
     1681 aatctgctgt ttatgatttt aaagcgtctt acgttcgtgc cgtcgcagac caaacagcaa
     1741 ctgctggtta tgtaaaaact aacactgcat acaccattac ttatcagtaa gtattcacca
     1801 attaaattga ttgaatgaat atacagggaa tagtgttaat aatttctatt tttatattat
     1861 tccctgtttt aattaactct gtcagggatg gtttatgttt tttaacacca aacatacaac
     1921 agctttatgc ttcgtaacct gtatggcttt tagttcatcg tctattgcgg acattgtcat
     1981 ttcgggtact cgcgtaatat ataaaagcga tcaaaaaagt gtcaacgtac gtctggaaaa
     2041 taaagggaat aacccgttgc ttgtccagag ttggttagat actggcgatg acaacgctga
     2101 gcctggcagt attacagtcc cttttactgc cacgccgcca gtatcgcgta ttgatgccaa
     2161 acgtgggcaa acaatcaaat taatgtacac agccagcacc tcactgccta aagacagaga
     2221 gagcgtgttc tggtttaacg tactggaagt tccaccaaaa ccagatgcag aaaaggtcgc
     2281 gaatcaaagt ctgctgcaac tggcatttcg cacacgtata aaacttttct atcgcccgga
     2341 tggattgaag ggaaatccct ctgaagcccc gttagccctg aagtggttct ggtcaggttc
     2401 agaaggtaag gcgtcattgc gagtgaccaa tccaacccct tactacgtct cttttagcag
     2461 tggtgattta gaagctagcg gtaaacgcta tccgattgat gtgaaaatga ttgcaccatt
     2521 tagtgatgag gtcatgaaag tcactggcct taatggcaaa gcaagttctg caaaagtaca
     2581 tttttacgcc attaatgatt ttggtggcgc cattgaaggt aatgccagtc tgtaatcagg
     2641 caaggatata attccgcagg aagcataacg tgactataaa accaactaaa aattatcata
     2701 atcatctgac ccgtatcgct acgttttgcg cgctgctgta ttgcaatagt gctttctgtg
     2761 ctgaacttgt tgaatatgac cataccttcc tgatggggca gaatgcctca aatattgatc
     2821 tcagccggta tagtgaaggt aaccctgcta taccgggtat gtacgacgtc agtgtttatg
     2881 tcaacgatca acctattatt aaccaaagca ttacatttat tgaaattgaa ggaaaaaaaa
     2941 acgcacaagc ctgtatcaca cttaagaatt tattgcagtt tcatataaat tctcccgata
     3001 taaataacga aaaagccgtt ctgcttgcca gggatgaaac actcggcaat tgccttaatt
     3061 tgacggaaat tatccctcag gcttctgttc gttatgacgt taacgagcaa cgtctggata
     3121 tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa ctatgttgat ccatcgttat
     3181 gggaaaatgg cattaatgcg gccatgttgt catacaacct caacggatat catagtgaaa
     3241 ctcctggtcg aagaaatgac agcatttatg ctgcttttaa cggtgggatg aatttaggtg
     3301 catggcgact gcgtgcctca ggcaactaca actggatgac cgattctggc agtaattatg
     3361 attttaagaa tcggtatata cagcgtgata tcgcctcgct gcgttctcaa ctcattcttg
     3421 gtgagtctta tacaacgggc gaaacctttg attctgtcag tatacgcggc attcgtttat
     3481 acagcgacag ccgcatgttg cctccgacat tagcgagttt tgcgcctatc attcatggcg
     3541 ttgctaatac caacgccaaa gtcactatta cgcaaggtgg ctataagatt tatgaaacga
     3601 ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc gtcaggttac ggcagcgatc
     3661 ttattgttac tgtcgaagaa tccgatggct caaagcggac attctcgcaa cctttttcat
     3721 cggtcgttca aatgttacgc cctggcgttg gacgttggga tattagcggc ggccaggtct
     3781 taaaagatga tattcaggat gagcctaatt tatttcaggc cagctactac tacggtctga
     3841 ataactatct aacgggttat actggtattc agattaccga taataactat accgctggtt
     3901 tgttaggtct tggtctgaat acttcagtcg gtgcattttc tttcgatgtg actcactcca
     3961 atgttcgtat accggatgat aaaacatatc agggacaaag ttatcgtgtt tcctggaaca
     4021 agttattcga agaaacaagt acttcactga atatcgcggc ctatcgctat tcgacacaga
     4081 attaccttgg tcttaatgat gcactaactc taattgatga agtgaaacat cccgaacaag
     4141 atcttgaacc gaaatccatg cgtaattact cgcgcatgaa aaatcaggtt acggtcagta
     4201 ttaaccaacc gctaaaattt gagaaaaaag attacggttc attttatctt tccggaagtt
     4261 ggtccgatta ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca
     4321 gtgcatcctg gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca
     4381 ctgacgatag cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac
     4441 aacggacttc aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata
     4501 accaactcaa cgtcagtagc agtggctata gtgataacgc tcgcctcagt tatagcgtaa
     4561 atactggcta tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca
     4621 gctatgaatc accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc
     4681 gtcaagtgtc tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca
     4741 gtaatgacag ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag
     4801 gagcgcgaat aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg
     4861 ctctttctcc ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg
     4921 ttgaattaaa aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg
     4981 attttgaaac cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa
     5041 atattccatt tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac
     5101 agggtggaca agcatttgtt cgtggtattg agcagcaggg aaatatcagc attaaatggc
     5161 tcgaagaaag taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa
     5221 aaatagcgca atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggga
     5281 cgtcaaatga taaaaataac gccacataaa atagcgatac tgatgggaat attattatca
     5341 ccatcagtat ttgcaacgga tattaatgta gagtttacag ccactgtcaa agcgacaacc
     5401 tgtaacatca cacttactgg taataacgtc acgaatgatg gcaataataa ctacacattg
     5461 agaatcccta agatgggtct ggataagatc gcgaataaaa cgacagaatc tcaggctgat
     5521 tttaaactgg ttgctagtgg gtgcagcagt ggcatcagtt ggattgatac cactctgacc
     5581 ggaaatgcat catcaagctc acctaagctt attataccgc agtctggtga ttcatcttcg
     5641 acgacaagta atatcggtat gggtttcaaa aaacggacta ctgatgatgc cactttcctt
     5701 aaacctaaca gtgcggaaaa gatacgctgg agcacagacg agatgcagcc cgataagggt
     5761 cttgaaatga ccgttgcgct acgtgaaaca gatgcagggc aaggcgtacc ggggaatttt
     5821 cgtgcactgg caacgtttaa tttcatctat caataagaag gataattaag atgacattta
     5881 aaaatttacg ttatggatta tccagcagtg tcgttttggc tgcctcattg ttcagcgtac
     5941 cctcttatgc ggcaacagac agcattgggc tgaccgtgat tactaccgta gaaataggta
     6001 cttgtactgc tacattagta aatgactctg atcaggacat ttctgttgtt gactttggcg
     6061 atgtatatat ttctgaaatc aatgccaaga ccaaagttaa aacattcaaa ctcaaattca
     6121 aagactgtgc gggtatcccc aataaaaaag cacaaataaa attaaccaag cgagccacat
     6181 gcgaaggtac tgctaatgac ggtgcggggt tcgcaaatgg atccacagcc gcagataaag
     6241 caagtgccgt cgccgttgaa gtctggagca ccgaaactcc ggcaacaggg agcgcaacac
     6301 aatttagttg tgtgacacca gcatctcaag aggtcactat ctccaatgca gccaacgcgg
     6361 ttgtttatta tccgatgagt gctcgactgg tcgtcgaaaa aaataaaacc gtaagcaatg
     6421 tcactgcggg taaattttct gcacctgcca catttacagt aacctataac taacagactg
     6481 acagatccag gaactatata tgcatcccac tcaacgtaag ctgatgaaga gaataattct
     6541 gtttctgtca ctacagtttc ccattaccta tccagccatc gctgggcagg atattgacct
     6601 tgttgccaat gtaaaaaaca gcacttgcaa aagcggaatc agtaaccagg gtaatattga
     6661 tcttggcgtc gttggggtgg gatatttttc agataatgtt actcctgaaa gctatcaacc
     6721 aggtggaaaa gagttcactg tcacagtatc agactgtgca ttacagggaa ctggcgatgt
     6781 gcttaatcag ttacatattg attttagagc ccttagcggt gtcatggctg caggttcaag
     6841 gcaaatattt gccaatgaag tcgcaaccgg ggctaaaaat gtcggtgtcg ttctcttttc
     6901 cattcaggac ccgaccaata tattcaatgt tattagttca gcaggaaatt ctcgttccgt
     6961 gtatccagta atgacttctg ccctgcataa ttcttcatgg aaattttatg ccagaatgca
     7021 aaaaatcgat cccgcgctgg atgtcatatc tggccaggta atgagtcata tattagttga
     7081 tgtgtactac gaataataga tcaaaacaaa taactgcgac tgttacagca gtagatggtg
     7141 gagtgataat gaagaaattc ttcagacatt ttcttttttt aattctgtgc ttgagttgtt
     7201 ataccgcgag tgctggcact gatgataacg taggatatat tgttgggaat agttacggcg
     7261 ttggtccgtc agatcaaaaa tggagagaaa cggggccgaa tggcgatgct actgtcatat
     7321 tcagatatgc aacaagtaca aataatttag ttttctacaa accgacgcag cttggaccta
     7381 caggtgtaaa attacagtgg agtcagttag ataccgcttc tggtggtggt tttctttatt
     7441 gcaacagaag tgatagcaca agtggtagcg caatgcgtat tgaaaatgca atggttgact
     7501 caggtaaaat gtatggctcc cataaattat ttaatacatc agttcctggt ttgtattaca
     7561 cattattaat ttcaaacatg tggtcagctt acggtaccgt aactaacgtt agttcacctg
     7621 ggatatacat tggtgactct gcagaacaat atttttcgtg gtataatcca agcgaagacg
     7681 tgttatattg gagttgcaat aatgcgaata gcacccgtaa atactgggct gtaggtggta
     7741 tttatcagac ccttacaatt gaattctata cagatacaaa ctttgatcca acagtaactc
     7801 aacagatcaa attatccagc tcatcaaatt atctgtattc atttaaagcc tatggcgcag
     7861 gccaaggtat aaatgagcat agttatttta tcaaaatcga tttcgatctg ctcaatgtca
     7921 agttgactaa tcccacttgt tttaccgcta tgcttagtgg aacttctgtg actggttcta
     7981 cggtaaaaat gggtgaatat agtgcagaac aaatcagaaa cggtgccaca ccggttcctt
     8041 ttgatatttc acttcaaaac tgcgttcgtg tgactaatat tgagacaaaa ttagtttcaa
     8101 caaaggttgg tactgaaaac gggcaactcc ttggtaatac tctcacaggt aatgacgcag
     8161 caaaaggagt cggtgtactc atagaaggtt tagcaactag taaaaatcct ctaatgacat
     8221 tgaaacctaa tgattcaaat tctgtttata aagattacga cccgagaggc aaagacgata
     8281 caacaggagg agtttacccg gatcaagata caggtataac ataccctctc catttccagg
     8341 ccacgctaca acaggatgga actataccaa tagaagctgg tgagtttaaa gccaccagta
     8401 ctttccaggt aacctaccct taataagtcc accgcaccgc catcatctgg cggtgcggta
     8461 attgataaat cattgccccc accaatgtga ctgataaaat gccaggcaac acgacatcat
     8521 ttatcaggac acgttatgaa accgaccacc attgcttcac ttcagaaatg taagcaggat
     8581 aaaaagcgtt tcgcgaccat caccgcttac gactacagct tcgcgaaact atttgctgat
     8641 gaaggactta acgtcatgct ggtaggcgat tcgctgggca tgacggttca gggccacgac
     8701 tccacccttc ccgttaccgt cgaggatatc gcctaccaca ctgccgccgt acgtcgcggc
     8761 gcgccaaact gcctgctgtt ggctgacctg ccgtttatgg cgtatgccac gccggaacaa
     8821 gcctttgaaa acgccgcaac ggttatgcgt gccggtgcca atatggtcaa aattgagggc
     8881 ggtgagtggc tggtcgaaac cgtaaaaatg ctgaccgaac gtgccgttcc tgtatgtggt
     8941 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt tcaggggcgc
     9001 ggcgatgaag cgggcgatca actgctcagc gatgcattag ccttagaagc cgctggggca
     9061 cagctgctgg tgctggaatg cgtgccggtt gaactggcaa aacgtattac cgaagcactg
     9121 gcgatcccgg ttattggcat tggcgcaggc aacgtcactg acgggcagat cctcgtgatg
     9181 cacgacgcct tcggcattac cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc
     9241 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
     9301 tatccgggcg aagaacacag tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc
     9361 tgccgctgct gcgtcagcaa attcgccgcc tgcgtatgga aggcaagcgc gtggcgctgg
     9421 tgcctactat gggtaatctg cacgatggcc atatgaagct ggtcgacgaa gccaaagccc
     9481 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac cgcccggaag
     9541 atctggcacg ttatccacgg accttgcagg aggactgcga gaagctaaac aaacgtaaag
     9601 tggatttagt tttcgcccct tcggtaaaag agatctaccc gaacggtact gaaacccata
     9661 cttacgttga cgttcccggg ctttcgacca tgctggaagg cgcgagccgt ccggggcatt
     9721 ttcgcggcgt ttcgactatc gtcagcaagc tgtttaacct ggtccagccg gacatcgcct
     9781 gcttcggtga aaaggacttt cagcaactgg cgctgatccg caaaatggtt gccgatatgg
     9841 gcttcgatat tgagattgtc ggtgtgccaa ttatgcgcgc caaagacggt ctggcgctaa
     9901 gttcccgtaa cggttatctg acggcggaac aacgcaaaat tgcgcctggt ctgtacaaag
     9961 ttttaagttc gattgctgac aaattgcagg ctggggaacg ggatctcgat gaaattatta
    10021 ccattgcggg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg
    10081 atgccgacac attgctggaa gtttctgaaa ccagcaaacg ggcagtaatt ctggtagccg
    10141 cctggcttgg cgatgctcgc ctgatcgaca acaaaatggt cgagctggcg taatacttaa
    10201 ctggcgctac ggctgatggc gccagttatt aatttacccc acgtcatctg tcataacaca
    10261 ggtaacttaa ataaggttaa ttaccttatc aatctcagca agagataaat ttgtcatctc
    10321 tgcaacgtct tcccgagaca ttcctttact tagaagacgc agggcgaatt cttgcctttc
    10381 ctcctgtctt ccctgctgaa tccccttctc aatccctttc tcttcaaacc actgcgccag
    10441 cgtcatcata gactcccctc ccgtttccct gtctctcagc acaccataaa acaaatccgc
    10501 ttgttcagta tgaccgcgtt gcagcatata gttttgcatg gcaactaact gacttccgct
    10561 agtgtaccct tcgtcaatca gcgtgaccag ttgctccagc aataacatta agtcgcgctg
    10621 gcgaatatgt ttttgcagta gttcgagaat cgcaatccgc cgatgttgca tgatttcgtc
    10681 atccggtgtg atggtgatat ccaccagcgg gaaaggactg ttatagacgc gtcgtgccag
    10741 ctccggcgag taaaatatat caaaccagca cattgagagc ggataaggtg tggcctcgcc
    10801 ctgataaaac agtatcggca ccaccagcgg cagcttatcg tgatccgcct ccagatgccg
    10861 gtgcatggca gcaatagaat aacgcatcat gcgaaaggcc attttcttat ccggcttgct
    10921 ttggtgttca atcacaacat gcagataacc gggattgccc tgcatttgca cggaatagag
    10981 cacgtccgtg ctgtgtcctt tcaggctctc ttcaatgaaa ctccccgact ctaaatgaag
    11041 cgtgttgagg tcacaaagtt ctcgtaattc cactggcaaa tgtatctcca gaaagtcgcg
    11101 agccgtctcc gcatgcatta aaaattgttt aaataccgcg tcatgcggtg tggtactcgg
    11161 tgcatccatc ttccctgtct tccgtgttgt cttgcgtgaa ggcagtatcc agtcaaggcg
    11221 cgccgtcgtc agccgcctct ttagtgttta ccgagcagcg ttcagagaaa tttcaattcg
    11281 ttacaggcga tacatggcac gcttcggcgc ggtacgcaaa ctgctgacgc aaatgccatc
    11341 agatgagtag acactcatca aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg
    11401 caccgcctgc ttcgttaacg acagggtaga aaggtagaag ttatgattcg cacgatgctg
    11461 cagggcaaac tccaccgcgt gaaagtgact catgcggacc tgcactatga aggttcttgc
    11521 gccattgacc aggattttct tgacgcagcc gg
//
LOCUS       DAFATA010000127        11270 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFATA010000127 DAFATA010000000
VERSION     DAFATA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.128"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..225
                     /locus_tag="LGJ20_004160"
     CDS             <1..225
                     /locus_tag="LGJ20_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="in Escherichia coli this periplasmic enzyme was
                     found to encode the periplasmic catalytic subunit of an
                     oxidoreductase; requires inner membrane anchor protein
                     YedZ; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mononuclear molybdenum enzyme YedY"
                     /protein_id="HBK2388163.1"
                     /translation="ERPPTTWNLAAPDEYGFYANVNPHVDHPRWSQATERFIGSGGIL
                     DVQRQPTLLFNGYADQVASLYRGLDLRENF"
     gene            226..861
                     /gene="msrQ"
                     /locus_tag="LGJ20_004161"
     CDS             226..861
                     /gene="msrQ"
                     /locus_tag="LGJ20_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBK2388164.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            1118..1768
                     /gene="zinT"
                     /locus_tag="LGJ20_004162"
     CDS             1118..1768
                     /gene="zinT"
                     /locus_tag="LGJ20_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBK2388165.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFPHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            2111..2641
                     /locus_tag="LGJ20_004163"
     CDS             2111..2641
                     /locus_tag="LGJ20_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBK2388166.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(3211..3300)
                     /locus_tag="LGJ20_004164"
     tRNA            complement(3211..3300)
                     /locus_tag="LGJ20_004164"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3264..3266),aa:Ser,seq:cga)
     gene            3394..4191
                     /gene="mtfA"
                     /locus_tag="LGJ20_004165"
     CDS             3394..4191
                     /gene="mtfA"
                     /locus_tag="LGJ20_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBK2388167.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLLRLHHANDTDSFSATNVH"
     gene            4292..4367
                     /locus_tag="LGJ20_004166"
     tRNA            4292..4367
                     /locus_tag="LGJ20_004166"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4325..4327,aa:Asn,seq:gtt)
     gene            4678..>11270
                     /gene="yeeJ"
                     /locus_tag="LGJ20_004167"
     CDS             4678..>11270
                     /gene="yeeJ"
                     /locus_tag="LGJ20_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HBK2388168.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLVFPMAAA
                     AQGVVNAAIQQPVPAQIAIANTNTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGALSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETTDGVYKATYTAYTKGSGLTAKLLMQSWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATIDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQYMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNGGKGMTDSNGVAIATLTGTLAGTHMITARLANSNVSDA
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPSNHPVAGITVTFTMPQDV
                     AANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDSQPVTFVADKTSA
                     QVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNE
                     SGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGT
                     PQNSSGSVITATVVDNNGFPVKGVTVNFTSRTNSAEMTNGGQAVTNEQGKATVTYTNT
                     RSSIESGARPDTVDASLENGSSTLSTSINVNADASTAHLTLLQALFDTVSAGDTTNLY
                     IEVKDNYGNGVPQQEVTLRVSPSEGVTPSNNAIYTTNHDGNFYASFTATKAGVYQVTA
                     TLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATVADTEGNAIANTE
                     VTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTASMTGGKSEQLVVN
                     FIADTLSAQVNLNVTEDNFIANNVGMTTLQATVTDGNGNPLANEAVTFTLPADVSASF
                     TLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIADAGTATLAS
                     LTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDQGFAEI
                     LVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPEGQLMVAQDVAVK
                     AHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVSMTPERNGSYMVKAS
                     LANGASLEKQLEAIDEKLTLTASSPLIGVYAPTGTTLTATLTSANGTPVEGQVINFSV
                     TPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSST
                     AHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVT
                     DQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADASQSVLKNNRSSLKGD
                     FTDSAELHLVLHDISGNPIKVSEGMEFVQSGTNVPYMKISAIDYSQNINGDYKATITG
                     GGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGA
                     YYQLNND"
BASE COUNT         3178 a         2711 c         2629 g         2752 t
ORIGIN      
        1 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
       61 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
      121 ggcggcatcc tcgatgtaca gcgccagcca acgctactgt ttaatggtta cgccgaccag
      181 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctgaatgcg tctgacagca
      241 aaacaggtga catggctgaa agttagcctg catcttgccg gattgttgcc gtttctctgg
      301 ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga tattcagcat
      361 tttaccggtc gcactgcact gaaatttttg ctggcgacct tgttaatcac acctctggca
      421 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
      481 gcctgggcga cactgcattt aaccagttac gcattgctgg agctgggcgt gaataatctg
      541 gcgttattag gcaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
      601 gttattttgc ttgctttagc gtttacttca acccaggcga tgcagcgaaa actcggcaag
      661 cactggcaac agttgcataa tttcgtctat cttgtcgcga tcctggcccc aatacattat
      721 ctgtggtcag tgaagattat ttcaccgcag ccgctcatct acgctgggct ggctgtactg
      781 cttttagcct tacggtataa gaagttgcgt tcccttttta accggttacg caagcaggtt
      841 cacaacaaat tatctgtata acttcatatt gccgacaaag tacgcgttat ccctgccgga
      901 tgtggcgcga gcgccctgtc cggcctgcgc agatcacatc aggttatgtt tcgtttgtca
      961 tcaacctcaa attatcaaca taattcattt tatttcaata agatagataa gtacacctga
     1021 gaaaactatg ctctcatctc ctaagagttg ttgcattttg ccatatgtta caatataaca
     1081 ttacacatca tatacaatga ctctggagga aactgttttg gcaattcgtc ttcacaaact
     1141 agctgttgct ttaggtgtct ttattgttag cgctcctgcc tttccgcatg gtcatcactc
     1201 acacggcaaa cccttaacag aggtcgaaca aaaagctgct aatggtgttt ttgatgatgc
     1261 caatgtacaa aaccgaacgc tcagtgactg ggatggagtc tggcaatccg tttatccttt
     1321 actgcaaagt ggcaaacttg accccgtctt tcagaaaaaa gcggatgcag ataaaaccaa
     1381 aacatttgct gaaattaaag attattatca caaaggttat gcgacagata tcgagatgat
     1441 tggcattgag gacggcattg ttgaattcca tagaaataat gaaacaacat cctgtaaata
     1501 tgattacgat ggatacaaaa tactcaccta taaatcaggc aagaaaggcg ttcgttactt
     1561 attcgaatgt aaagatcctg aaagcaaagc ccctaaatat atacaattta gcgatcatat
     1621 aattgcacca cgaaaatcat ctcattttca catttttatg ggtaatgatt cacagcaatc
     1681 tttattaaat gaaatggaaa actggccaac gtattatcca tatcagttga gtagcgaaga
     1741 agtggtcgag gaaatgatgt ctcattgagt attctcatga taacgcctcg atgccgcttt
     1801 agtaagtcat cataactgcc actggtccat ccacaaacgc cactgaacgc aagctagcta
     1861 cagacacgct catcactatg acgtgtctgt atattaataa gctaacccgc attgagttaa
     1921 ccaataacgg attccataca caatacggcc aacagagaaa gatacctgtg ctcacgccat
     1981 tgcttatatt ggctggttac aatgtgcact atcaattttt taaataacaa attcttaaaa
     2041 ccacttctgt ataaggaatt tttaaggttc gtgggatagc ttgactgtga aaattacagg
     2101 agctacaaaa atgaaccgat tctcaaaaac tcaaatttat ttacattgga taacgctgct
     2161 tttcgttgca ataacctatg ccgcgatgga actccgtggc tggtttccta aaggtagtag
     2221 tacttatctg ctgatgcgag aaacacatta caatgcgggt atattcgttt gggtgttaat
     2281 gttttcacgc ctgattataa aacaccgtta tagtgatcct tctattgtac caccgccacc
     2341 tgcctggcaa atgaaagcgg cttcgctaat gcacatcatg ctttatataa ccttccttgc
     2401 acttcctctg ctggggattg ctttgatggc ttacagtgga aaatcgtgga gtttccttgg
     2461 tttcaatgtg tctccctttg ttaccccaaa cagcgaaatt aaagcactga taaaaaatat
     2521 tcacgaaacc tgggcaaata taggttactt tttaatcgca gctcacgctg gcgcagcact
     2581 ctttcatcac tacattcaga aagataatac tctgttacga atgatgcctc gccgcaaata
     2641 agtcgatccc ctgacaagga aatgtgaacc atggacatca acgcgtcata aatgtttacg
     2701 caagtgcaca atgcaccctt tccactatca acaaggagga gcagaaactg tgtaaatcgt
     2761 acaatggtaa gacttatcaa aaatacaggt taattttggt atttaaacct tttttacttg
     2821 ttagtatgat acaaaggctt tcaaaaaagc tgggaaattc aacaactctt ggaaattatg
     2881 gactttgtcc cgcggcttta atctgacggc cgcgttcctt ttttggctca aatattgacc
     2941 ccttctctac gcaacttcag ttcccaccac caactttaca gcagtttcat aggatgaatg
     3001 tctaaaagaa gaatggtgac cgataagaaa acgactgaat aactgcacat attcgctcga
     3061 aactttcctg tcagatccat agcgaatcaa gtgctgaatg tcacagtatc gaacagaaaa
     3121 cagtgacgat ctaacccttc aagaatattc tacgattgtt ctgtttagga aaagcaaggc
     3181 gggaagtcgg gagataagtc attgataaag tggcggagag agggggattt gaacccccgg
     3241 tagagttgcc cctactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc
     3301 gttcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa
     3361 ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa
     3421 gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg
     3481 ggtctgacgg aacaggaaca aagcaaatta gtcactcttg ccgaacgttt tttacagcaa
     3541 aagcggcttg ttcctttaca gggctttgaa ctggattcat taagaagctg ccggatagca
     3601 cttctatttt gcctacccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa
     3661 gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg
     3721 gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg
     3781 aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa
     3841 gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttattccg
     3901 ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa
     3961 gaaatcgaat tggttggcga gaatgcggcg agcattgatg cttatgctgc cagtgatcct
     4021 gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct
     4081 cgtttccctt cattgtggca acgtttctgc caattttatc aacaagatcc tttgctgaga
     4141 ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta attaacaact
     4201 ttgcagatta attaaccaat tgaaatgact tatgaaattt agtgttgaca gacaaggtac
     4261 cgctaagtaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     4321 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tctaaaaatt
     4381 cgcttcttag cgcaatgtca ctgaccttaa ttgagcattg tttttcaacg gatagcgggt
     4441 tttgacatct taagcgccct cgacctttat ggttgagggc gttttgctat ggcgccatca
     4501 ccattttccc ctcgattata aaacttgagt aattcagtag tctcccctct tgcaactcac
     4561 acccaaaact gcctaacgaa aagttattaa ttttcaatca tattgctatc aggatttaca
     4621 ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa ggtaagcatg
     4681 gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatcttatg cgggatgggt
     4741 atcaaactac gccgcttaac tgcgggtatc tgcctggtaa ctcaacttgt gttccctatg
     4801 gctgcggcag cacaaggtgt ggtaaacgcc gcaatccaac aaccagttcc tgcacaaatc
     4861 gccattgcaa ataccaatac ggtgccctac accctaggag cgctggaatc ggcccaaagc
     4921 gttgccgaac gtttcggtat ttcggtggct gagttacgca aactcaacca atttcgtacg
     4981 tttgctcgag gttttgataa tgtccgccag ggtgatgaac tggatgtccc ggcacaagtt
     5041 agtgaaaaaa aattaacccc gccgccgggt aatagcagtg acaacctcga gcaacagata
     5101 gccagtactt cacagcaaat cgggtctctg ctcgccgagg atatgaacag cgagcaagcg
     5161 gcaaatatgg cgcgtggatg ggcctcttct caggcttcag gcgcaatgac agactggtta
     5221 agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagattttag cctgaagaac
     5281 tcccagttcg attttctcca tccgtggtat gaaacgcctg ataatctctt tttcagtcag
     5341 catactctcc atcgtactga cgagcgtacg cagattaaca acggcttggg ttggcgtcat
     5401 ttcactccca catggatgtc gggcatcaac ttctttttcg accacgatct tagccgttac
     5461 cactcccgcg ccggcattgg cgcggagtac tggcgcgact atcttaaatt aagcagtaac
     5521 ggctatttgc gactgaccaa ctggcgcagc gcacctgaac tggacaacga ttatgaagca
     5581 cgcccggcca atggctggga tgtacgcgca gaaggctggt tacccgcctg gccgcacctt
     5641 ggcggtaaac tggtctatga acagtattat ggcgatgaag tggccctgtt cgataaagac
     5701 gatcggcaaa gtaatcctca tgccataacc gctggactaa actatacccc cttcccgctg
     5761 atgaccttta gcgcggagca acgccagggt aaacagggcg aaaatgacac ccgatttgcc
     5821 gtcgatttta cctggcaacc tggcagcgca atgcagaaac agcttgaccc gaatgaagtc
     5881 gctgcacggc gtagccttgc aggcagccgt tatgatctgg tggatcgcaa caacaacatc
     5941 gttctggaat atcgcaaaaa agaactggtc cgcctgaccc tgacagaccc cgtgacaggg
     6001 aagtcaggag aagtgaaatc actggtttcg tctctacaaa ccaaatatgc cctgaaaggc
     6061 tataacgtcg aagccaccgc tctggaagct gccggtggta aagtggttac aacgggtaaa
     6121 gatattctgg ttaccctgcc ggcgtaccgg ttcaccagta cgccagaaac cgataacacc
     6181 tggccgattg aagtcaccgc tgaagatgtc aaaggcaatt tttcgaatcg tgaacagagc
     6241 atggtagtcg ttcaggcacc tacgctaagc cagaaagatt cctcagtatc attaagtacc
     6301 caaacattga acgcggattc ccattcaacc gccacactga cttttattgc tcatgatgca
     6361 gcaggtaatc ctgttatcgg gctggtgctt tcgacgcgtc acgaaggagt tcaggacatc
     6421 accctgtctg actggaaaga taatggtgac ggaagctata cccaagtcct gaccacagga
     6481 gcgctgtctg gcacgctgac gcttatgcca cagctgaacg gtgtggatgc ggctaaagcc
     6541 cccgccgtgg tgaatatcat ttctgtttcg tcatcccgga ctcactcgtc aattaagatt
     6601 gataaggacc gttatctctc cgggaatcct atcgaggtaa cggtagaact gagagatgaa
     6661 aatgacaaac ctgttaagga gcaaaaacag caactgaata ccgcagtcag catcgacaac
     6721 gtgaaacctg gtgtcactac agactggaaa gaaaccacag atggcgtcta taaggcaacc
     6781 tataccgcct ataccaaagg cagtgggctt actgcgaagc tattaatgca aagctggaat
     6841 gaagatttgc ataccgctgg atttatcatc gacgccaacc cgcagtcagc gaaaattgcg
     6901 acattatctg ccagcaataa tggtgtgctc gccaatgaga atgcagcaaa caccgtctcg
     6961 gtcaatgtcg ctgatgaagg aagcaaccca atcaatgatc ataccgtcac gtttgcggta
     7021 ttaagcggat cggcaacttc ctttaacaat caaaacaccg caaaaacgga tgttaatggt
     7081 ctggcgacta ttgatctgaa aagtagtaag caggaagaca acacggttga agtcaccctt
     7141 gaaaatggcg tgaaacaaac gttaatcgtc agttttgtcg gcgactcgag taccgcacag
     7201 gttgatctgc agaagtcgaa aaatgaagtg gtcgctgacg gcaatgacag cgccacaatg
     7261 accgcgacag ttcgggatgc aaaaggcaac ctgctcaatg acgtcaaggt caccttcaat
     7321 gttaattcag cagaggcgaa actgagccaa accgaagtga atagccacga cgggatcgcc
     7381 acagctacgc tgaccagttt gaaaaacggt gattatacgg ttacggcctc tgtgagctct
     7441 ggttcccaag ccaatcaaca ggtgaatttt attggtgatc aaagtactgc tgccctgacc
     7501 ctcagtgtgc cttcaggtga tatcaccgtc accaacacag ctccgcaata tatgactgca
     7561 accttgcagg ataaaaatgg caacccacta aaagataaag aaatcacctt ctctgtgcca
     7621 aacgacgtcg caagtcggtt ctcgattagc aacggcggaa aaggcatgac ggatagtaac
     7681 ggggttgcaa tcgccaccct gaccggcacg ttagcgggca cgcatatgat cacggctcgt
     7741 ctggctaaca gcaatgtcag cgatgcacag ccaatgacgt ttgtggcgga taaagacaga
     7801 gcggttgtcg ttctgcaaac atcgaaagcg gaaatcattg ggaatggcgt ggatgagaca
     7861 actctgacag caacagtgaa agatccgtcg aatcatccgg tggcgggaat aacggtgacc
     7921 ttcaccatgc cacaggacgt tgcggcaaac tttaccctcg aaaataacgg cattgccatc
     7981 acccaagcca atggggaagc gcatgtcacg ctgaaaggta aaaaagcggg tacgcatacg
     8041 gttaccgcaa cgctgggtaa taacaatacc agtgattcgc agccggtaac gtttgtggcg
     8101 gacaaaacct cggctcaggt tgtcctgcag atgtcaaaag atgagatcac aggtaatggc
     8161 gtcgataacg caacgctaac tgcaacggtt aaagatcagt tcgacaatga ggtgaataat
     8221 cttccggtaa cattcagctc agcctcttca ggactcaccc tgaccccggg agtaagtaat
     8281 accaatgagt ctggcatcgc gcaggccact ctcgcaggcg ttgcctttgg tgagcagacg
     8341 gtcactgcat cactggctaa taatggtgcc agcgacaaca aaactgtgca ttttattggc
     8401 gacacagcag cggcaaaaat tatcgagttg acgcctgtcc cagacagcat aatcgccggt
     8461 accccgcaga acagctccgg cagcgtcatc accgccacag tcgttgataa taatggcttt
     8521 ccggtgaaag gtgtgactgt gaacttcacc agcagaacaa actctgccga aatgacgaat
     8581 ggcggccaag ccgtaacgaa cgaacagggt aaggctaccg tcacttatac caatacccgc
     8641 tcctcgatag aatcaggagc gagaccggat accgttgatg ccagtctgga aaatggtagc
     8701 tccacgctta gcacatcaat taatgtcaac gctgatgcgt ctacggcaca tctcaccttg
     8761 ctacaggcac tttttgatac agtctccgca ggcgacacta ccaatctgta tattgaggtg
     8821 aaggataatt acggcaacgg tgtaccccag caggaggtaa ccctcagagt atcaccaagt
     8881 gaaggcgtga cccccagtaa taacgctata tatactacca accacgacgg caatttttac
     8941 gcaagcttta ccgctacaaa agccggggtt tatcaagtga cggcaaccct cgaaaatggc
     9001 gattcgatgc aacaaacagt gacctatgtg ccgaacgtcg cgaatgccga aatcacgctg
     9061 gcagcctcga aggatccggt gattgccgac aataacgatc tcacgacact aacagcaaca
     9121 gtcgctgata cagagggcaa tgcgatagcc aacactgaag taacatttac tctgccggaa
     9181 gatgtgaagg cgaacttcac gctgagcgat ggcggtaaag cgattactga tgctgaaggc
     9241 aaagcgaaag tcacgctgaa aggtacaaaa gcaggcgctc atactgttac agcatcgatg
     9301 actggcggta agagtgagca gttggtggtg aactttattg cggatacgct cagtgcgcag
     9361 gttaatctta acgttaccga ggacaacttt atcgccaata acgttgggat gaccacactt
     9421 caggcaacag tgactgatgg aaacggcaac ccgttagcca atgaggcggt gacattcacg
     9481 ctaccggcag acgtgagcgc aagcttcact ctcggacaag gcggttccgc cattactgat
     9541 atcaacggca aggctgaagt tacactgagc ggtacaaaat ccggcaccta ccccgtgaca
     9601 gttagcgtga acaattatgg tgtcagtgat acgaaacagg tgactttgat tgccgatgct
     9661 ggtaccgcaa cactagcctc cttaacctct gtatactcat tcgtcgtcag cacgaccgag
     9721 ggcgcgacca tgactgcaag cgtcactgac gctaacggca acccggtaga aggcataaaa
     9781 gttaatttcc gcggaacctc cgtcacgcta agcagcacca gcgttgaaac ggatgatcag
     9841 ggtttcgctg aaattcttgt gacaagcacc gaagtcggac tgaaaacagt ttcagcctct
     9901 ctggcagata aacctactga agtcatatcg cgattactga atgcaaaagc agatattaat
     9961 tctgcaacga ttaccagtct ggagatacct gaaggtcagc taatggtcgc acaagacgta
    10021 gcagttaaag ctcacgtcaa cgaccagttt ggcaacccga ttcttaatga atctgtaaca
    10081 ttcagtgcag aaccaccaga gcacatgacc atcagccaaa atattgtctc tactgatacg
    10141 catggtatag ccgaggtctc catgacgccc gaaagaaacg gttcgtatat ggtgaaagca
    10201 tccctggcga atggagcctc acttgagaaa caactggagg ctattgatga aaaactgaca
    10261 ctcacggcgt ccagtccgct tatcggtgtc tatgccccta caggcactac tctgacggca
    10321 acgctaacct ctgcaaatgg cactccagtg gagggtcagg tcatcaactt tagcgtaacg
    10381 ccagaagggg cgacgttaag tggcggaaaa gtgagaacta actcttcagg tcaggctccg
    10441 gtcgttctga ccagcaataa agtcggtaca tatacggtga ctgcatcgtt ccataacggc
    10501 gtaacaatac agacacagac aaccgtgaaa gtcactggca actcaagcac cgcacatgtt
    10561 gctagcttta tcgctgatcc atcgactatc gccgccacca acagtgattt aagtacctta
    10621 aaggcaacgg ttgaggatgg cagtggtaac ctgatcgaag gtctcactgt gtacttcgcc
    10681 ttaaaaagcg gctctgccac attaacgtca ttaacagcgg tgaccgatca aaacggaatc
    10741 gcgacaacaa gcgtgaaagg agcgatgaca ggtagcgtca cggtaagcgc agtcacgacc
    10801 gctggtggaa tgcaaacagt agatataacg ctggtggcag gcccggcaga cgcctcgcag
    10861 tccgtcctta agaacaatcg gtcctcactg aaaggggact ttaccgatag tgctgagcta
    10921 catcttgttt tgcacgatat atcaggcaat ccgatcaaag tttctgaagg gatggaattt
    10981 gtgcaatcag gtactaacgt gccctatatg aaaattagcg caattgatta cagtcaaaat
    11041 atcaacggtg attacaaagc caccattaca ggcggcggag agggtatcgc aacgctgatc
    11101 cctgtattga atggtgttca tcaagctggt ctgagtacca caatacaatt cactcgcgca
    11161 gaagacaaaa taatgagcgg tacagtatca gtcaatggta ctgacctacc gacaactaca
    11221 ttcccttcgc aggggttcac cggggcgtat tatcagttga ataatgacaa
//
LOCUS       DAFATA010000128        11268 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFATA010000128 DAFATA010000000
VERSION     DAFATA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.129"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1292)
                     /locus_tag="LGJ20_004168"
     CDS             complement(<1..1292)
                     /locus_tag="LGJ20_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK2388169.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRV"
     gene            complement(1279..2244)
                     /gene="iaaA"
                     /locus_tag="LGJ20_004169"
     CDS             complement(1279..2244)
                     /gene="iaaA"
                     /locus_tag="LGJ20_004169"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK2388170.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            2448..3683
                     /gene="moeA"
                     /locus_tag="LGJ20_004170"
     CDS             2448..3683
                     /gene="moeA"
                     /locus_tag="LGJ20_004170"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBK2388171.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            3683..4432
                     /gene="moeB"
                     /locus_tag="LGJ20_004171"
     CDS             3683..4432
                     /gene="moeB"
                     /locus_tag="LGJ20_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBK2388172.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(4611..5273)
                     /gene="fsa"
                     /locus_tag="LGJ20_004172"
     CDS             complement(4611..5273)
                     /gene="fsa"
                     /locus_tag="LGJ20_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK2388173.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQAVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            5404..6303
                     /locus_tag="LGJ20_004173"
     CDS             5404..6303
                     /locus_tag="LGJ20_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBK2388174.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            6309..8741
                     /locus_tag="LGJ20_004174"
     CDS             6309..8741
                     /locus_tag="LGJ20_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBK2388175.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            8887..9702
                     /gene="ybiV"
                     /locus_tag="LGJ20_004175"
     CDS             8887..9702
                     /gene="ybiV"
                     /locus_tag="LGJ20_004175"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBK2388176.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            9854..11119
                     /locus_tag="LGJ20_004176"
     CDS             9854..11119
                     /locus_tag="LGJ20_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBK2388177.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTADQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         2661 a         3074 c         2946 g         2587 t
ORIGIN      
        1 tgtacacgca gcggttcgat aatcgaatca ccgatggtct gacgtgggtc cagcgaagcg
       61 taagggtcct gaaaaataaa ctgaatatcc cggcgcaatg cctgaagttt gccgggtgac
      121 aaggtatcga ttctctgacc gttaaagata atttcgccgc cctgcgattc gaccaggcgc
      181 agcaacgccc gcccggtagt ggatttaccg ctgccagact cgcccaccag cgatagcgtt
      241 tcgccaggcc agagatcaaa actgactttc tcaacggcat gcacttcccg cgttacgcga
      301 ttcaacaaac cgctgcgcaa agggaaacgg gtgacaagat tacgcactcg taaaacaggt
      361 tcgccatcca ccaccgtttt ctgctcgatg gggggggcct gtttcgctgg atgttcaagc
      421 gatatcaacg ggaaacgtcg gggataatct aaccctttca tcgcaccaag ttgcggaaca
      481 gcagctaaca gcgcacgggt gtaaggatgt tgcggtgcat gaaaaatctg ttcgacggta
      541 cccgtttcca ccgcctcgcc ctgatacatc accagtaccc gatcggcaat ctctgccacc
      601 acgcccatat cgtgagtgat aaagataacg cccatcgaca tctctttttg caatactttg
      661 attaattgca ggatctgcgc ctgaatagtg acatccagcg cggtggttgg ctcatcggca
      721 atcagcaccg ccgggcggca tgacagcgcc atcgcaatca tcactcgctg gcgcatcccg
      781 ccagagagtt gatgcggata acgtgaaaga atggtttgtg cctcaggaat gcgtacctga
      841 tccagcatcc gcttcgcctc gaccatcgct tcttcacgac tggcgttctg atgcagacga
      901 attgattcgg caatctgttc acccacagta aataccgggt tcagcgatgt catcggttcc
      961 tgaaatatca tcgccatatc cgcaccgcga acatggcgca tttgtgcagc gctctgctcg
     1021 ctaagttcaa tcacttcgcg actgcgccgc tgcaacagca ttttatcgca ctgtactaaa
     1081 ccgcccgcct gttccaacag gcgcatcaat gccaacgcag tcactgactt accggagccg
     1141 gattcgccaa caattgccag cgtctcaccg cgttgcagac taaaagagag attgcggacc
     1201 gcagctattt tctgctggtc ctgcataaag gcaatattca gattttcaac cgccagcaca
     1261 ttaccggcat caagttcatc actgtgtggc aacggtgtcc cctttttcac ggtagatacc
     1321 ggtggttggc gtatcgcctg cgtagcccca ggcgcgatac attccttcgg tgttaaacgg
     1381 tagcgcgaca ttcccttcat ggtcgatagc gattaagcca ccgctaccgc caagcgcagg
     1441 gagtttttcc attactaccc gctcgcaggc ttccgcgaga cttaatccgc cgtaatccat
     1501 taacgcggcg atgtcatatg ccgccagcgc gcggatgaag acttcgcccg tgccggtaca
     1561 agaaaccgcc acactggcgt tattggcgta gcatccggca cccactaagg gactatcgcc
     1621 aactcgtccg ggtaatttat tggtcattcc gcctgtggac gtggctgccg ccaaattgcc
     1681 gtctaaatcc aacgccacgg cccccacggt gcccattttc tgtttttcat ccagtggcgc
     1741 accgctatgg tcgaggacgg ttgccccttc cttgcgcgct gccagtagtt gttcataacg
     1801 caaaggcgtg gagaaaatct ccggcgagac gcgctccatg ccacgagcaa acgcaaaatt
     1861 ttctgcccct tcgccaatca tcatcacatg cgggctttgc tccatcacca gccgggcggc
     1921 aagaaccgga ttacgcagat gactaacgcc cgccaccgca ccggctttca gggtgttacc
     1981 atccatcaca caggcgtcca gttcatgggt ttcatcacgc gtaaagacag cgccaattcc
     2041 ggcgttaaac agtggacact cttccagcag acgcaccgct tccgtcacca catccagcgc
     2101 actttcgccc gcttccagca ttttctgccc ggtttcaaca atggcagaca acgcctcaat
     2161 gtagcgtaat tcctgttgca gactcatctg cgcgcggcta attgcacctg cgccaccatg
     2221 aattgcaatg actgctttgc ccatagtatt cgtccattaa gggtggctcg ccacttttgc
     2281 tataaatatc agaattttta tgaattactc attcaatttt tgaatataga aagatggata
     2341 tggcatgtaa aggcaggagc caacgctacg cccgaggtgg catacagtct gtggcgtttt
     2401 tctgacataa taggcaaatt cgattttgcc tccgcaggag tgttttcatg gaatttacca
     2461 ccggattgat gtcgctcgac accgcgctta atgagatgct ttctcgcgtc accccactga
     2521 ccgcccagga aacgctgcca ctggtacagt gttttggtcg tattctggcg agcgatgtcg
     2581 tttcgccgct tgatgttccg gggtttgata actccgcaat ggacggctac gcggtgcgtt
     2641 tagccgatat tgcctccagg caaccgctgc ccgttgccgg taaatccttt gccggtcagc
     2701 cgtaccatgg tgaatggcct gcgggtacct gcattcgtat tatgaccggt gcgccggtgc
     2761 cggaaggctg cgaagcagtg gtgatgcagg agcagactga acaaacggac aatggcgtgc
     2821 gttttactgc tgaagtgcgt agcgggcaaa atattcgccg tcgcggtgaa gatatctctg
     2881 caggtgcggt tgttttcccg gcgggaactc gcctgactac cgctgaactg ccagtgattg
     2941 cttcactggg gattgccgaa gttccggtga ttcgtaaagt gcgtgtagcg cttttttcta
     3001 ccggtgatga actccagttg cccggtcagc cactgggcga tggccaaatc tacgatacca
     3061 accgtctcgc cgtacacctg atgttagaac agttgggatg cgaggtaatc aacttaggga
     3121 ttatccgcga cgatccacat gccctgcgcg ccgcatttat tgaagccgac agccaggcgg
     3181 atgtggtgat cagttccggc ggtgtttcag tgggtgaagc ggattacacc aaaaccattc
     3241 ttgaagagct gggggagatc gccttctgga agctggcgat taaaccgggt aaaccgttcg
     3301 cgttcggtaa actttctaat agctggttct gcggcctgcc gggcaacccg gtttcagcga
     3361 cgctgacctt ctatcaactg gtacagcctt tgctggcaaa actaagcggc aacaccgcca
     3421 gcggcctgcc cgcgcgccag cgcgtacgca cagcgtcccg cctgaaaaaa acgccaggac
     3481 gtcttgattt ccagcgcggc gtgctgcaac gcaacgccga cggtgaactt gaagtgacga
     3541 ccaccggaca tcagggttca catatattta gctcctttag tctcggcaac tgctttatcg
     3601 tgctggaacg cgatcgcggc aatgtggacg tgggcgaatg ggtggaagta gaaccgttta
     3661 acgcgttgtt cggaggcctg taatggcgga actcagcgat caggagatgc tgcgctacaa
     3721 ccggcaaatc attctgcgcg gttttgattt tgacggtcag gaggcgctaa aagattctcg
     3781 tgtgctggta gtcggcctcg gtggactcgg ctgcgcagct tcgcagtatc tggcaagcgc
     3841 cggtgtcggc aacctgacgc tgctcgactt cgacacggtt tcgctctcga atctgcaacg
     3901 ccagacactg cacagtgatg ccacggtcgg gcaaccgaag gtggaatccg cccgttacgc
     3961 cctgacgcgg atcaacccac atatcgcgat tacgccagtc aatgcactgc tagatgacgc
     4021 agaacttgca gcattgattg ctgaacacga tctggtgctc gactgtacgg ataacgttgc
     4081 ggtacgtaat caactgaacg caggctgttt tgccgcgaag gtgccgctgg tttccggcgc
     4141 ggcaattcgt atggaagggc aaatcacggt cttcacttat caggacggcg aaccgtgcta
     4201 tcgctgcctt agccgtttgt ttggcgagaa cgcattaacc tgcgtagaag caggcgtgat
     4261 ggcaccgttg atcggcgtaa ttggttcgtt gcaggcaatg gaagcgatca aactgctggc
     4321 aggttatggc aaaccggcca gcgggaaaat cgtcatgtac gatgcgatga cctgtcagtt
     4381 tcgcgaaatg aaactgatgc gcaatccggg gtgtgaggtg tgcgggcagt aattgccacc
     4441 tccaggcctg ataagacgcg acaagcgtcg catcaggcat ttgctccaga cgccagatgc
     4501 ggcgtgaacg ccttatctgg cctacggttc gggcacagat ttccaagcgc ggcaggcatc
     4561 ggtgcactcc gccacgtcat cagcaattta tgaggatgca gaacgtccgg ttaaatcgac
     4621 gttctgccaa acgctcccag ccagtcctgt tcaaacttcg ccacagcggc atcaaccgcc
     4681 ggatagctaa tcatctgttg tgccacatcc agtggcagcg taattgattc acatcctgcc
     4741 agtaagcagt ccagcgcctg acgcggggtt ttgaaactcg ctgccagcac tttcgcctgc
     4801 ggcgcatgca ttttcaataa ctggtgtaag tcggtcacag cctgaatgcc gctaccgccc
     4861 tgagcatcaa tacgattaac gtaaggcgca acatattccg cacctgccag cgccgacagc
     4921 agcccttgtg ctgcgccata taccgcggtt cccagcgttg gaatcccttc cgcttttaac
     4981 atcttaatag ctgccagccc ctcggcggtc accggaactt tcaccacgat atccgcaata
     5041 atagaacgca atttacgtgc gtcattcacc atcccttcag cagtggtagc cattacctgg
     5101 gcaaacagac gcccctgacc gcccatcgct tcatgaagtt gcggaagcac aacatccagc
     5161 ggttttttac ccgcggcgat aatgcttggg ttagtggtca cacccgccag cggaaaaata
     5221 cgtgacagcg ccttcaccgc aacaacgtct gaagtatcca gatacagttc cataaccatc
     5281 ctcaaaatgt actttttgtc tgaatgtaga ctatcacggg aaaactctct gaacagttga
     5341 cgcacatcaa tctaactttc attcgaaagt attttaatgt ttgaacgaaa cagagaggca
     5401 actatgattt tcaatattca gcgctactcg acccatgatg gccccggtat ccgcacggtc
     5461 gtatttctta aaggctgttc gctgggctgc cgctggtgtc agaacccgga aagccgcgcc
     5521 cgcacgcagg atctgctgta tgacgcacga ctgtgtctgg aaggctgcga gctgtgcgct
     5581 aaggccgcgc cggaagtgat tgagcgcgcg ctgaatggtt tgcttattca tcgggaaaag
     5641 ttaaccccgg agcatctgac ggcgttaacc gactgctgtc cgacacaggc attaaccgtg
     5701 tgtggtgaag tgaaaagcgt tgaggagatc atgacgaccg ttctgcgcga taaaccgttt
     5761 tatgatcgca gcggcggcgg tttaacgctt tcgggtggtg aaccctttat gcagccggaa
     5821 atggcgatgg cgctactgca agccagccac gaggccggca ttcatactgc ggtagaaacc
     5881 tgtctgcatg tgccgtggaa atatatcgcc ccttctctgc cctatatcga tctgtttctt
     5941 gccgatttaa aacacgttgc cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc
     6001 agggtgctgg ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg
     6061 ccgctgattc agggctttaa tgccgacgaa acctctgtaa aagccattac cgattttgcc
     6121 gccgacgagc tgcacgttgg cgaaattcat tttctgccct accacacgct gggcatcaac
     6181 aaatatcact tacttaatct gccctatgac gccccggaaa aaccgcttga tgcgccagaa
     6241 ctgctcgact ttgcccagca gtatgcctgc cagaaagggt taaccgcgac cttacgagga
     6301 taacaaccat gaccacactg aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa
     6361 atgcgctggt gcatattgtg aaaccgccag tctgtaccga gcgcgcgcag cactataccg
     6421 agatgtatca acaacatctc gataagccga tcccggtacg tcgcgcgctg gcactggcgc
     6481 atcacctggc gaatcgcacc atctggatca aacacgatga gttgatcatt ggcaaccagg
     6541 caagcgaagt tcgcgccgcg ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag
     6601 agattgatga tctggcagat cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac
     6661 gcgttctgca tgaagtgtgc ccgtggtggc gcggtcagac cgtacaggat cgctgctacg
     6721 gcatgtttac cgatgagcaa aaaggtctgc tggcgaccgg aatcattaaa gcggaaggca
     6781 atatgacctc cggcgatgcg cacctggcgg tgaatttccc gctgctgctg gaaaaaggtc
     6841 ttgatggtct gcgtgagaaa gtagcggaac gtcgctcgcg catcaatctg acggtgctgg
     6901 aagatttaca tggcgaacaa ttcctgaaag cgattgatat cgtgctggtg gcagtcagtg
     6961 aacacattga acgtttcgct gccctggcgc gtgaaatggc tgctaccgaa actcgcgaaa
     7021 gccgtcgcga tgaactgctg acgatggcag aaaactgcga tcttatcgcc caccagccgc
     7081 cgcagacttt ctggcaggcg ctgcaactgt gttacttcat ccaattgatt ttgcagatcg
     7141 aatctaacgg tcactcggta tcgtttggtc gtatggacca gtatctctac ccgtactatc
     7201 gccgcgacgt tgaactcaac cagacgctgg atcgcgaaca cgccatcgag atgctacata
     7261 gctgctggct gaaactgctg gaagtgaaca agatccgctc cggctcacac tcaaaagcct
     7321 ctgcgggaag tccgctgtat cagaacgtta ccattggcgg acaaaatctg gttgatggtc
     7381 aaccaatgga tgcagtgaat cccctctctt acgcgatcct cgaatcctgc ggtcgcctgc
     7441 gttccactca gcctaacctc agcgtgcgtt accatgcagg aatgagtaat gatttcctcg
     7501 acgcctgcgt acaggtgatc cgttgcggct tcgggatgcc ggcgttcaac aacgacgaaa
     7561 tcgtgatccc ggaatttatt aaactcggta ttgaaccgca ggacgcttac gactacgcag
     7621 cgattggttg tatcgaaacc gccgtcggtg gcaaatgggg ctatcgctgt accggcatga
     7681 gctttatcaa cttcgcccgc gtgatgctgg cggcgctgga aggcgggcgt gatgccacca
     7741 gcggcaaagt gttcctgcca caagaaaaag cgttgtcggc aggcaacttt aacaatttcg
     7801 atgaagtaat ggacgcgtgg gatacgcaaa tccgttatta cacgcgtaaa tcaatcgaaa
     7861 tcgaatatgt cgtcgacacc atgctggaag agaacgtgca cgatattctc tgctcggcgc
     7921 tggtggatga ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact
     7981 gggtttctgg cctgcaggtt ggcattgcca acctcggcaa cagcctggcg gcagtgaaga
     8041 aactggtatt tgaacagggc gcgattggtc agcaacagct ggctgccgca ctggcggatg
     8101 acttcgatgg cctgactcat gagcaactgc gtcagcggct gattaacggt gcgccgaagt
     8161 acggcaacga cgatgatact gtcgatacgc tgctggctcg cgcttatcag acctatatcg
     8221 acgaactgaa acagtaccat aatccgcgct acggtcgtgg tccggttggc ggcaactatt
     8281 acgcgggtac gtcatctatc tccgctaacg taccgtttgg cgcgcaaact atggcaacgc
     8341 cggacgggcg taaagcccac accccgctgg cagaaggcgc tagtccggcc tctggtactg
     8401 accatctcgg ccctactgcg gtcattggct cggtgggtaa actgcctacg gcagcgattc
     8461 tcggcggcgt gttgctcaac cagaaactga atccggcaac gctggagaac gaatctgaca
     8521 agcagaagct gatgatcctg ctgcgcacct tcttcgaggt gcataaaggc tggcatattc
     8581 agtacaacat cgtttcccgc gaaacgctgc tggaggcgaa aaaacatccg gatcagtacc
     8641 gcgatctggt agtgcgtgtc gcgggttatt ccgccttctt caccgcgctc tctccagacg
     8701 ctcaggacga tatcatcgct cgtactgaac atatgctgta atcttttcgg cccggctgcg
     8761 gcgtcgccgg gctaaatcat tcacatcatc aatttcatcc ttactttcat tcaaaatata
     8821 atttgtgctc tgcgtcacat tgttattaca ctgttacggt ttcattttcg agccaggagc
     8881 acacctatga gcgtaaaagt tatcgtcaca gacatggacg gtacttttct taacgacgcc
     8941 aaaacgtaca accaaccacg ttttatggcg caatatcagg aactgaaaaa gcgcggcatt
     9001 gagttcgttg tcgccagcgg taatcagtat taccagctta tttcgttctt tcctgagcta
     9061 aaggatgaga tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa
     9121 ctgttccacg gcgaactgac ccgacatgaa tcgcggattg ttattggcga gttgctaaaa
     9181 gataagcaac tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc
     9241 cccgaagcat ttgtcgcact gatggcaaaa cactaccatc gcctgaaagc tgtaaaagat
     9301 tatcaggaga ttgacgacgt actgttcaag ttttcgctca acctgccaga tgaacaaatc
     9361 ccgttagtga tcgacaaact gcacatagcg ctcgatggca ttatgaaacc cgtcaccagt
     9421 ggttttggct ttatcgacct gattattccc ggtctacata aagcaaacgg tatttcgcgg
     9481 ttactgaaac gctgggatct gtcaccgcaa aatgtggtag cgattggcga cagcggtaac
     9541 gatgcggaga tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac
     9601 attaaacaaa tcgcccgtta cgctaccgat gataataatc atgaaggcgc gctgaatgtg
     9661 attcaggcgg tgctggataa cacatcccct tttaacagct gacctctcta ccggagccgt
     9721 gcgctccggt tttcccttct ttttacatcc tgtacatcac atcaaatcat taaacttgca
     9781 ttaacttatt tataacttat attgtcacat gcaaatcaga atggtttcga atgaaagtta
     9841 atgagaggtg gttatggctt ctacttttac cagcgacaca ttgcctgccg atcacaaagc
     9901 agctatccgt cagatgaagc acgcgctgcg ggcgcagctt ggcgacgtcc agcagatctt
     9961 taatcagcta agcgatgaca ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca
    10021 gggcgatgcc gtctggccgg tgctgtctta tgccgatatc aaagcaggtc atgttactgc
    10081 agatcagcgc gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg
    10141 cgaacaagcg ctaggctggg atcagtcgat gctggactat ctggaccgca accgctttga
    10201 cgaggtctac aaaggccccg gcgataattt cttcgggacg ctcagcgctt cacgtcccga
    10261 gatttacccc atctactggt cgcaggcgca aatgcaggcc cgccagagtg aagaaatggc
    10321 gaatgcgcag tcgtttctca atcgtctgtg gacatttgaa agtgatggaa agcaatggtt
    10381 taacccggat gtgagcgtca tctaccctga ccgtatccgc cgccgtccgc ccggaacgac
    10441 ctccaaaggt cttggagcgc ataccgactc cggggcgctg gaacgctggc tgcttccagc
    10501 gtatcagcgc gttttcgcca acgtctttaa tggcaatctg gcgcaatatg atccctggca
    10561 tgcggcacat cgtacggaag ttgaagagta cacggtggac aacaccacca aatgttccgt
    10621 gtttcggaca ttccagggct ggacagcgct ctctgatatg ctgcctggtc aggggctgct
    10681 gcacgtcgtg cccattcctg aagccatggc gtacgtactg ttacgtccgc tgcttgatga
    10741 tgtgccggag gatgaactgt gcggcgtagc gcccggaaga gtattgccgg tatcagagca
    10801 atggcatcca ctgttgattg aggcgttaac cagcattcca aaactcgaag ccggagactc
    10861 cgtctggtgg cactgcgacg tcatccattc cgttgccccc gttgaaaatc aacagggctg
    10921 gggcaacgtg atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg cctacgcgca
    10981 caaggtgaag gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta
    11041 tgaaacaaac tgggaaggac gctttacgct tgccgacctc aacattcacg gtaagcgagc
    11101 gttgggcatg gatgtttgag agttaatgga agtgaattga tgtgggatta agaaaatcga
    11161 ttcgacgtta ctcagattgc tgacaagtgc gctttcttaa tgcctgatgc ggcattaacg
    11221 ccttatctgg cctacaaagt cctgcagatt caatatatta caggtact
//
LOCUS       DAFATA010000129        10712 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFATA010000129 DAFATA010000000
VERSION     DAFATA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.130"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..473
                     /locus_tag="LGJ20_004177"
     CDS             <1..473
                     /locus_tag="LGJ20_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388178.1"
                     /translation="KTAADYEFSSSASWVDVDATGKVTFKNVGSNWERITATPKSGGP
                     SYVYEIRVKSWWVNSGDAFMIYSLAENFCSSNGYTLPRADHLNHSRSRGIGSLYSEWG
                     DMGHYTTEAGFQSNMYWSSSPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNI"
     gene            complement(736..1788)
                     /locus_tag="LGJ20_004178"
     CDS             complement(736..1788)
                     /locus_tag="LGJ20_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBK2388179.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVMSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCFSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDMPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            2103..3419
                     /gene="shiA"
                     /locus_tag="LGJ20_004179"
     CDS             2103..3419
                     /gene="shiA"
                     /locus_tag="LGJ20_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBK2388180.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANITHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            3521..4975
                     /gene="amn"
                     /locus_tag="LGJ20_004180"
     CDS             3521..4975
                     /gene="amn"
                     /locus_tag="LGJ20_004180"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBK2388181.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            5318..6034
                     /locus_tag="LGJ20_004181"
     CDS             5318..6034
                     /locus_tag="LGJ20_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK2388182.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6487..6562)
                     /locus_tag="LGJ20_004182"
     tRNA            complement(6487..6562)
                     /locus_tag="LGJ20_004182"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6527..6529),aa:Asn,seq:gtt)
     gene            6889..7806
                     /gene="nac"
                     /locus_tag="LGJ20_004183"
     CDS             6889..7806
                     /gene="nac"
                     /locus_tag="LGJ20_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBK2388183.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7908..8858
                     /gene="cbl"
                     /locus_tag="LGJ20_004184"
     CDS             7908..8858
                     /gene="cbl"
                     /locus_tag="LGJ20_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBK2388184.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPGVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(8896..8971)
                     /locus_tag="LGJ20_004185"
     tRNA            complement(8896..8971)
                     /locus_tag="LGJ20_004185"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8936..8938),aa:Asn,seq:gtt)
     gene            8976..10619
                     /gene="yeeO"
                     /locus_tag="LGJ20_004186"
     CDS             8976..10619
                     /gene="yeeO"
                     /locus_tag="LGJ20_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBK2388185.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT         2736 a         2330 c         2530 g         3116 t
ORIGIN      
        1 gaaaaacggc ggctgattat gagttttcaa gctctgcctc ctgggtcgat gttgatgcta
       61 ccggtaaagt gacatttaaa aatgtcggca gcaattggga aaggattacg gcgacgccaa
      121 aatcaggagg ccctagctat gtatacgaaa tccgtgtgaa gagttggtgg gtgaactccg
      181 gcgatgcttt catgatatac agccttgctg aaaatttttg cagtagcaat ggctacacac
      241 ttccccgtgc agaccattta aaccatagtc gttcccgagg catcgggtca ctgtacagtg
      301 aatggggaga tatggggcat tacacgactg aagctggttt tcaatcaaat atgtattggt
      361 catctagtcc cgcaaactca agcgaacaat acgtagtttc cctggcaaca ggtgatcaaa
      421 gcgtatttga aaagcttggg tttgcttatg cgacatgtta taaaaacatc tgattttcct
      481 ctataatttc ttaaagagtg ctccattcgg ggcactcttt ttttacattc cctctataga
      541 gcttgctctt ctctcacaga aaatgaataa ataaaaatgc gataccgcca gattcgcgtt
      601 cggtgtgact atgatcattt tcggtgagta cagctttgat ccaaaggctg gttttctgtg
      661 atgattgacc agcctcagag tattgatgac accaccgttc ataacagatc ggcattacta
      721 tgcataagta ctgtctcata ttctcgccaa aacacttttt aatgcctttg cgaattcatc
      781 cagacttata ttgtcaattt tttgcccaac gacgactgac tccgaaacat cggccattgc
      841 gggccatcgc gttttgtaat cagcataaat tgcaacaacg ggagtattat aggcacttgc
      901 catatgtaat attgaggtgt caggagttat tgcgatataa gcatctttta tgattgctgc
      961 tgaacgttta atcgagggtg gtaaggataa acggtagaca ttattataac aatccactaa
     1021 tgccctggct ttatcttcac ctttgggtcc atgaacgatc actattggca tatctgtttc
     1081 actttgaatt tttgctatta gattttctgc aatcgataat gaaaatgtac gttcctgcga
     1141 gctaccttct aaattgaacg caatgtaaga tcctaacgat cgcatttcct cgcgcacctc
     1201 atcgagtaca tcctcactta gtggcaattc atatattggc ctgactgctg gaaaacccgc
     1261 ctcacgcatc ataaatgccc atgtcatagg tacgggagcc cggagtttct gatccatacg
     1321 ggacgcgttc ttgcacaaag gggagaagca attcatggtt ataccaacaa cctgaaaatt
     1381 cgtttttgct ttcaactgac tgataaataa catcgttttc aggttctttt tacgcatccc
     1441 ctcaatgcaa agatccggcg taccgtattg ctgtgttatg ttctttgcta aatcttttat
     1501 ttcttttaat gttgcgtgat cctgcatagt cattgtgact aatgttaatt tagtctgttc
     1561 aagtttaagc gcattaaaga cttctaaatt aattgtcgac attacaatta aaagatgctt
     1621 aattttatgc aattcaagcg cccgaataac aggaaagatg gccatagcat cgccaatctg
     1681 atcgggaata tggatgacaa caaagtctgt tttttcaata ttgaaattat aagctttata
     1741 atcgtagtaa ctaaatgcaa tacgtctcaa caatgatgct aaaaacatac ctaacctcgc
     1801 ctccctactg gttataatgc aatgcagtct atcagactca tcagggtgcc attttgtgca
     1861 tatgcggact tttatgtttc atatctctaa cctgtgggtc ctctgcttaa tccttaaaca
     1921 acaccagcaa ctcctgcgct ttcatcttcc atcgaatttt tcatgttgcc gctaatcagc
     1981 cataaaatca tttgcagatg cgctctgtcg aggtagtctc ataaggttcg tttatagatc
     2041 gacggcaatg tgagttacct tttccatact aattataaaa agacagtaca aacaggatca
     2101 ttatggactc cacgctcatc tccactcgtc ccgatgaagg gacgctttcg ttaagtcgcg
     2161 cccgacgagc tgcgttaggc agcttcgctg gtgccgtcgt cgactggtat gattttttac
     2221 tctatggcat caccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg
     2281 cgatgggaac gctcgccgca tttgctacct ttggcgtcgg attccttttc cgtccgctcg
     2341 gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga
     2401 ccgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca
     2461 ttgggtggtg ggcacctatt ttgctggtga cactgcgtgc cattcaggga tttgccgtcg
     2521 gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag
     2581 ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt tcaaccggac
     2641 tggtttcatt gatcagtatg atgacgactg acgaacagtt tttaagctgg ggctggcgca
     2701 ttcctttcct atttagcatc gtactggtac tgggagcatt gtgggtgcgc aatggcatgg
     2761 aagagtccgc ggaatttgaa caacagcaac attatcaagc tgccgcgaaa aaacgcatcc
     2821 cggttatcga agcgctgtta cgacatcccg gtgctttcct gaagattatt gcgctacgac
     2881 tgtgcgaatt gctgacgatg tacatcgtta ctgcctttgc acttaattat tcaacccaga
     2941 atatggggct accgcgcgaa cttttcctta atattggttt gctggtaggt ggattaagct
     3001 gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tggtcgccgt agggtttata
     3061 tcacaggtgc gttaatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac
     3121 aatctatttt ctggatagtt ttcttctcca taatgctggc aaacattacg catgacatgg
     3181 tggtgtgtgt gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg
     3241 gcgctggagt cggttatcag gttgccagtg tggttggcgg tggatttaca ccttttattg
     3301 ccgctgcact catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg
     3361 ctggatgcct gatttccgca atgaccgctt tgttgatgaa agacagtcaa cgcgcttgat
     3421 agcctggcga agatcatccg atcttcgcct tacacttttg tttcacattt ctgtgacata
     3481 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
     3541 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc
     3601 attacgcaac gccattggca actatattac cagtggcgaa ttacctgatg aaaacgcccg
     3661 caaacaaggt ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaaccaa
     3721 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
     3781 tactcgccct actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga
     3841 ttatggtgcg catatctctg tgcaaccctc gcagcatgaa atcccttatc cttatgtcat
     3901 cgatggctct gaattgacac ttgatcgctc aatgagcgct gggttaactc gctacttccc
     3961 gacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaacgga
     4021 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg
     4081 ccattatacc ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac
     4141 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
     4201 ctacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga
     4261 agaagccatt tccgaccttg catggaaaaa acatcagatg ccagcatggc atttaattac
     4321 cgccgatggt cagggtatta ctctggtgaa tattggcgtg ggaccgtcaa atgctaaaac
     4381 catctgcgat catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg
     4441 cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga
     4501 tgaccacgtt cttgatgcgg ttctgccgcc cgatattcct attccgagca ttgctgaagt
     4561 gcaacgtgcg ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagtcaa
     4621 acagcggcta cgtactggta ctgtggtaac cacagatgac aggaactggg aattacgtta
     4681 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
     4741 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
     4801 agataaaccg ttgcatggcg agattaaact tcccggccag gctaaccgtt tttatgaagg
     4861 cgctatttcc gaacatctgc aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
     4921 cgaccgactg cattcgcgta aattacgaac ctttaatgag ccgccgttcc gataataagt
     4981 tctgaacctt cttatcagaa atagatgtga aggagtaagt aagacacctg gcaaatagcc
     5041 tgcaataatc gtgggctgtt tgcttccttg ggcggatacg agttttatta tcgtcttaat
     5101 gatttccaca tattaaaagc aagtatgctt tcaaaacaca attataaaaa atcccgccaa
     5161 caatataagt ttttataaaa ttaaatataa gattatggct ttagaatatt tttatttcta
     5221 atagacgaga tttttcctgt tatgatataa tatgctgaat taacacatgt taacgattta
     5281 ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat gggccaatat
     5341 tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa aattcggtgt
     5401 agaaatctat gctgctgcta aacaaggtga acccgatcca gaattaaaca catctttaaa
     5461 attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg ataaagcaat
     5521 tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag ggacgttatg aaggctttgg
     5581 tcctaatggc tcaatgatta tcgccgagac attgacttca aatgttaacc gtacgattgc
     5641 taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag gttctgtcag
     5701 ctatatgttt gacaatacgg gtgtgattgt atttaaagga tcagaccctg accatatttt
     5761 tgaaatttta cttgaagctg aagttgatgt tcgtgatgtg actgaagaag aaggtaacat
     5821 tgttatttat accgaaccta ctgaccttca taaaggaatc gcggctctaa aagcagctgg
     5881 aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag ttgagctttc
     5941 cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg acgacgatgt
     6001 acaaaaagtt tatcataacg tcgcaaatct ctaattatat tttaaagaaa tctgtcttta
     6061 cggcagattt ctttaatctc atataaccct tataaaaaat ataatgttca actcgtcata
     6121 ttgattatga aaaatccccg ttcccagaga aataatattt attaaaattc cagttcttct
     6181 ttttctgatt acagaaggca aagtggcaat aacgcatagt ttcccggtaa agacgcgata
     6241 gcgacatccc gcataaggca tttttctctt tatctttgta cggtacttca tggaacagag
     6301 tttttgacct tgcgaatcgt gatgtctgtt ggggggcaac ttgctcactg aagcgtgaga
     6361 ctcgattaag cgcacgaaac acagaaatca aaaaacccgg tcactttttt acaaggtaac
     6421 cgggtaaaaa taatttttat tttttaactg ttttgagact catagagatg tctcaaaact
     6481 aaaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt
     6541 ctaccgactg aactacagag gaatcgtgtg aacggggcgc atattaatgg cagtgcatgg
     6601 tgatgtcaaa gggtgaaatg catttttttg tttgtttgct gtcaatttca acaaagtggt
     6661 gagctttcaa tcttattggg taatgaacca tcgtggtgca taccctcctt ttatagggca
     6721 ggggaacgcg acagctgatt aaaggagcaa atgacgaatc tatcataatc tcttatttga
     6781 cagttggtta gcttgtacat caacaccaaa ataaaactgg caagcatctt gcaatctggt
     6841 tgtaagtaat ggcggcactt gggccgattc ttaaaaaccg gaggcaacat gaacttcaga
     6901 cgcctgaaat acttcgtaaa aattgtagat attggtagcc tgacccaggc tgctgaagta
     6961 ttgcatatcg cacaaccagc gctcagccag caggttgcca cactggaagg tgagttaaat
     7021 caacaacttt tgatccgtac aaagcggggc gttacaccaa cagacgccgg aaaaattctc
     7081 tatacccatg cgcgggccat tttacgtcag tgtgaacagg cccaactggc ggtgcataac
     7141 gttggtcagg cattatcggg gcaagtctcg attggctttg caccaggaac cgctgcgtca
     7201 tccatcacca tgcccttatt acaggcggtt cgcgctgaat ttccggagat cgttatctat
     7261 cttcatgaaa atagtggtgc agtgcttaac gaaaaattga taaatcacca actcgatatg
     7321 gcggtgattt atgagcattc ccctgtggct ggtgtatcca gtcaggcttt gctgaaagaa
     7381 gatctttttc tggtaggaac tcaagattgc ccggggcaaa gcgttgatgt gaatgctatt
     7441 gcgcaaatga acctctttct ccccagtgat tacagtgcta ttagacttcg tgttgatgag
     7501 gctttttccc tacggcgact cacggcaaaa gttattggtg aaattgagtc tattgccacg
     7561 cttaccgcag cgattgccag cggcatgggc gttgcagtat tacccgaatc ggccgcgcgt
     7621 tcgttatgtg gcgcagtaaa tgggtggatg tcacgcatta ccactccttc catgagtctc
     7681 tctttgtcat taaatttacc cgccagagcg aacttatcgc cacaggcaca ggcagtgaaa
     7741 gagttgttaa tgtcagtgat tagttctcca gtgatggaaa aaaggcagtg gcaattggtg
     7801 agctaagcgt tatatcggga tggaataaga tgcgggtttt tattatttgt tatgccgggc
     7861 gttagacttt aacaataacg ggatatctga actgcccgga gtttaccgtg aatttccaac
     7921 aactaaagat aatccgcgaa gctgcacgtc aggattacaa cctgacagag gttgcgaata
     7981 tgctttttac ctcacagtca ggcgtcagcc gtcatattcg ggaactggag gatgaacttg
     8041 gcatcgaaat atttgttcga cgaggtaagc gactgctggg catgactgaa ccgggcaaag
     8101 cattactggt cattgcagaa cgtattctga atgaagccag taatgttcgt cggcttgcag
     8161 acctgtttac caacgatacg tctggcgttc tcactattgc aacgacgcat actcaggcgc
     8221 gttatagctt gccaggggtc attaaagctt ttcgcgaact tttcccggag gttcggctcg
     8281 agctaatcca ggggacgcca caggaaattg cgacgttgtt gcaaaatggc gaagctgata
     8341 ttggtatcgc cagcgagcgt ttgagtaatg acccgcagct cgtcgccttc ccgtggtttc
     8401 gttggcacca tagtttgctt gttccactcg atcatccctt gacgcaaatt acaccattga
     8461 cgctggaatc aatagcgaag tggccgttaa tcacttaccg acaggggatt acggggcgct
     8521 cacgtattga tgacgcattt gcccgcaaag gtttgctggc agatattgta ttaagtgcgc
     8581 aggattctga tgtcattaaa acctatgttg ctcttgggct ggggatcgga ttagttgccg
     8641 aacaatccag cggtgaacaa gaggaaaaga atttaatccg cctggatacg cggcatcttt
     8701 tcgatgctaa tactgtctgg ttgggactca agcgaggaca acttcagcgt aactatgtct
     8761 ggcgctttct ggaactttgt aatgcaggac tttcagttga ggatatcaag cggcaggtga
     8821 tggaaaacag tgaagaggaa attgattatc agatatagca aaaagcccgc tataaaagcg
     8881 ggcttttcag aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
     8941 tccgccgttc taccgactga actacagagg aatcgctgtt gaggcacatc ttaacggcga
     9001 aaaatctttt gtcaaacccg atttttaaat tccccaactg tttgccgttt ctatcaacag
     9061 tttgttgcat ttgcagacaa tttgttggcg aaaatctttg cagctttgct gattctccct
     9121 cattatttga aatgtggttt cactttctgc aattaaggtc ggctttgaat atctcctctg
     9181 ctttacgcca ggttgttcac ggcactcgct ggcacgctaa acgcaagagc tacaaagtgt
     9241 tgttctggcg cgagataacc ccgcttgctg ttcctatctt catggagaat gcctgtgtcc
     9301 tgttgatggg ggttctgagc acttttctgg tcagctggct gggaaaagat gcgatggccg
     9361 gcgtgggatt ggcggacagc ttcaatatgg tcattatggc tttttttgct gctatcgatc
     9421 ttggtactac tgtcgttgtg gcatttagtc tcggtaagcg ggatcgacga cgagcgaggg
     9481 tggcgacgcg gcagtcattg gtgatcatga cgttgtttgc cgtactgttg gcaacgctta
     9541 ttcatcattt tggcgaacaa attattgatt tcgtcgcggg tgatgccacg acagaagtta
     9601 aagcactggc gttgacttat ctggagctga cggtactcag ttatccagca gctgccatca
     9661 ctttgattgg tagcggggca cttcgtggtg cagggaatac gaaaataccg ctactgatta
     9721 acggtagcct gaatattctt aatattatta ttagcggcat attgatttac ggccttttct
     9781 cctggccggg actgggattt gtcggggctg ggttgggttt aaccatttct cgttatattg
     9841 gcgcagttgc aattttgtgg gtgctggcga ttggttttaa tcctgcgcta aggatttcgt
     9901 taaagagcta ttttaaaccg ctgaatttta gcattatctg ggaagtcatg gggattggta
     9961 ttcccgcgag tgtcgaatca gtgttattta ccagtggtcg gttattaacc caaatgttcg
    10021 ttgccgggat ggggaccagt gttattgccg gaaattttat cgcgttttca attgcggctc
    10081 ttatcaactt acccggaagt gcgctcggct ctgcttctac gatcattaca ggccgaaggt
    10141 tgggggtagg gcagatagcg caagcagaga ttcagttgcg gcatgtgttc tggctttcca
    10201 ctcttggatt aacggccatc gcctggctaa cggctccctt tgccggggtt atggcatcgt
    10261 tttacaccca ggatccacag gttaaacatg tcgttgtgat tctgatttgg ctaaatgctt
    10321 tatttatgcc tatttggtcc gcctcatggg tgctacccgc tggatttaaa ggtgctcgtg
    10381 atgcccgtta cgccatgtgg gtttcgatgt tgagcatgtg gggttgtcgg gttgtagtcg
    10441 gttatgtgct gggaatcatg cttggctggg gtgtggttgg tgtctggatg ggaatgtttg
    10501 ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat ggttactgga cgttggctat
    10561 ggaaataccc tcgacccgag ccgcaaaagt gtgaaaaaaa gccagttgtg tcggaataaa
    10621 cgacaaaatg cagattattt cagcaaacga tttcaaattt aaaaaacagg ctttgacatt
    10681 gtgggtgggc atcgctaata ttcgcctcgt tc
//
LOCUS       DAFATA010000130        10628 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFATA010000130 DAFATA010000000
VERSION     DAFATA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.131"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..659
                     /locus_tag="LGJ20_004187"
     CDS             <1..659
                     /locus_tag="LGJ20_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBK2388186.1"
                     /translation="ERLRKEIAELRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFC
                     LMDVSGSMDQSTKDMAKRFYILLYLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQE
                     TGGTIVSSALKLMDEVVKERYNPAQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVV
                     RYYSYIEITRRAHQTLWREYEHLQSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD
                     "
     gene            806..2281
                     /locus_tag="LGJ20_004188"
     CDS             806..2281
                     /locus_tag="LGJ20_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2388187.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            2462..3952
                     /locus_tag="LGJ20_004189"
     CDS             2462..3952
                     /locus_tag="LGJ20_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2388188.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3995..4498
                     /gene="yeaK"
                     /locus_tag="LGJ20_004190"
     CDS             3995..4498
                     /gene="yeaK"
                     /locus_tag="LGJ20_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBK2388189.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(4499..4603)
                     /locus_tag="LGJ20_004191"
     CDS             complement(4499..4603)
                     /locus_tag="LGJ20_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388190.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            4773..5219
                     /locus_tag="LGJ20_004192"
     CDS             4773..5219
                     /locus_tag="LGJ20_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK2388191.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(5176..5997)
                     /gene="nimR"
                     /locus_tag="LGJ20_004193"
     CDS             complement(5176..5997)
                     /gene="nimR"
                     /locus_tag="LGJ20_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBK2388192.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            6094..7275
                     /gene="nimT"
                     /locus_tag="LGJ20_004194"
     CDS             6094..7275
                     /gene="nimT"
                     /locus_tag="LGJ20_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBK2388193.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGGDKEI
                     R"
     gene            7330..7677
                     /locus_tag="LGJ20_004195"
     CDS             7330..7677
                     /locus_tag="LGJ20_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBK2388194.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(7699..7953)
                     /gene="yoaF"
                     /locus_tag="LGJ20_004196"
     CDS             complement(7699..7953)
                     /gene="yoaF"
                     /locus_tag="LGJ20_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBK2388195.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            8136..9161
                     /gene="dgcP"
                     /locus_tag="LGJ20_004197"
     CDS             8136..9161
                     /gene="dgcP"
                     /locus_tag="LGJ20_004197"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBK2388196.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            9194..9292
                     /locus_tag="LGJ20_004198"
     CDS             9194..9292
                     /locus_tag="LGJ20_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBK2388197.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            9295..9369
                     /gene="yoaJ"
                     /locus_tag="LGJ20_004199"
     CDS             9295..9369
                     /gene="yoaJ"
                     /locus_tag="LGJ20_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBK2388198.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(9428..9676)
                     /locus_tag="LGJ20_004200"
     CDS             complement(9428..9676)
                     /locus_tag="LGJ20_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK2388199.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9904..>10628)
                     /locus_tag="LGJ20_004201"
     CDS             complement(9904..>10628)
                     /locus_tag="LGJ20_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2388200.1"
                     /translation="IQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQK
                     NQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMI
                     VIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQV
                     LAVPPAYTSQRYACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLAC
                     GEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2660 a         2542 c         2448 g         2978 t
ORIGIN      
        1 aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa aattgaacgc gtccctttta
       61 ttgacacctt cgatttacgt tacaagaact acgagaagcg gcccgatccc tccagccagg
      121 cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga tcaatccact aaagatatgg
      181 ctaagcgttt ttatattctg ctgtatctgt tcctcagcag aacgtataag aacgtggaag
      241 tcgtatacat ccgccatcat acccaggcga aagaagtcga tgaacatgag tttttctact
      301 cgcaggaaac aggcggcacc attgtttcca gcgccctgaa actgatggat gaggtagtga
      361 aagagcgtta taacccggca cagtggaata tttacgctgc acaagcatcg gacggcgata
      421 actgggccga tgactctccg ctttgccatg aaatcctggc gaaaaaatta ttacctgttg
      481 ttcgttatta cagctatatc gaaattaccc gtcgtgcaca tcagacattg tggcgagaat
      541 atgagcatct gcaatctact ttcgacaact ttgcgatgca gcacatccgc gaccaggatg
      601 atatttatcc ggtgttccgt gaactgtttc ataaacaaaa tgcaacagct aaagactaaa
      661 actatcagcc aggtcattat cgcctggctg atttttagct tactgtaaat tatctcattt
      721 attacataca ttagcttaca atcgctttaa atatgacagc ataaccttta cataatttag
      781 ttccagaaaa caatcattcg gaaaaatgat tcagtcaaca cgtatttcca tggggttatt
      841 ctttaaatat tttttatcgt taacgaaaat tgatcctggt caaaactata tatctctgcc
      901 atcaataaaa tccagcactc acattgctct cctttttatg gtttctatgg gtacacaaaa
      961 attaaaagct caaagctttt ttattttcag tttattgctg acgttaattt tattttgcat
     1021 caccacatta tataacgaaa acacaaatgt aaaactcatc ccacagatga attacctgat
     1081 ggttgttgtg gctttgtttt tccttaacgc cgtcattttt cttttcatgt taatgaaata
     1141 tttcactaac aaacaaattt taccaacact cattttaagc cttgcatttt taagtggcct
     1201 tatctattta gttgaaacca ttgtaattat ccataaacca attaacggca gtacactgat
     1261 ccagacaaag tcgaatgatg tttctatttt ctatattttc cgccaactca gttttatttg
     1321 tttaacctcg ctggcactct tttgttatgg aaaagacaac atccttgaca acaataagaa
     1381 aaaaacggga atcctgttgc tggcgctgat tcctttttta gtttttcccc ttctggcaca
     1441 caatctgagc agttataacg ctgactattc tttgtatgtc gtcgactact gtccggacaa
     1501 ccatactgcg acctggggaa tcaactatac aaaaatattg gtttgtctgt gggcattttt
     1561 actgttcttt attatcatgc gtacacgatt agccagcgaa ctatggccgt taatagcatt
     1621 attatgtctg gcatcgctat gctgcaactt acttctactg actctggatg agtataatta
     1681 caccatctgg tatatcagtc gcgggattga agtttccagt aaactgtttg ttgtgtcttt
     1741 tctgatttat aacatttttc aggaactgca actctccagc aaactggcag ttcatgatgt
     1801 gctgaccaat atttataatc ggcgctactt tttcaacagc gtagagtcat tattgtcgcg
     1861 acctgttgtt aaggacttct gtgtcatgct ggttgatatt aatcagttca aacgcatcaa
     1921 tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt tcaattgtcg atattatcca
     1981 gcaaagcatc cgccctgatg atattttagc gcgactggag ggtgaggtgt ttggcttgct
     2041 atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg gaacgtatgc gtaaaaatgt
     2101 cgaactcctg accggcttta gtaacagata tgatgttcct gaacaaatga ccatcagtat
     2161 tggcacggtt ttttcaacgg gtgacacgcg taatatctcg cttgtcatga cggaagcaga
     2221 taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa gtgattattc atcatattta
     2281 agtgcaaaaa tattcagagc catgcttttt gcgtggcttt tgcatacaat ttattactat
     2341 tacccctaaa tttcccctcc ccagcggcgc agagatgagt ataattagcg cccctgtgcc
     2401 aggccgcaat cgaactttat ctggttttct cgtttcacta accgaaggag tgccatttat
     2461 catgaaattg caccatagaa tgctccggca ttttatcgcc gcaagtgtca ttgtgctgac
     2521 atcttccttc cttatttttg aacttgtcgc cagcgacaga gcaatgagtg cctatctgcg
     2581 ctatatcgtg cagaaagcag actcctcctt tctttatgat aagtatcaga atcagagtat
     2641 tgccgcgcat gtgatgcgcg ctctcgctgc tgagcagtcg gaagtgtcgc cagaacagcg
     2701 gcgcgccatc tgcgaggctt ttgagtctgc caataatacc catggcttaa acctgactgc
     2761 ccataaatac ccgggcttac gcggcacact acaaaccgca tccactgact gcgacacaat
     2821 tgtggaagct gcagcactat tacccgcttt tgatcaggca gtggaaggca accgccacca
     2881 ggatgattac ggttcaggtc ttgggatggc cgaagagaaa tttcactatt atctcgatct
     2941 caatgaccgc tatgtctatt tttatgagcc ggttaatgtt gaatactttg cgatgaataa
     3001 ctggtccttc ctgcagtcag gaagtattgg catcgatcgc aaagatattg aaaaggtatt
     3061 taccgggcgt accgtattgt cgagcattta ccaggatcag cgtactaaac agaacgtgat
     3121 gagtttgctg acgccggtat atgtcgcagg gcagctaaaa gggattgtgc tgctggatat
     3181 taacaaaaac aatttgcgga atatctttta cactcatgac cgccctctcc tctggcgttt
     3241 tctcaatgtc acgctaaccg ataccgattc ggggcgcgac attatcatca accagagcga
     3301 agataatctg ttccagtatg tcagttacgt ccatgactta ccgggcggca ttcgtgtctc
     3361 gttatccatt gatattcttt actttatcac gtcttcgtgg aaaagcgttc tgttctggat
     3421 tttgacggcg ttaattttgc tgaatatggt gcggatgcac ttccgcttat accaaaatgt
     3481 gtcgcgagaa aatattagtg atgcgatgac tggactgtat aatcgcaaaa ttttaacccc
     3541 tgaactggag cagcggttgc agaaactggt gcaatccggt tcttcggtga tgtttattgc
     3601 aattgacatg gacaagttaa agctaataaa tgacaccctc ggtcatcagg aaggggattt
     3661 agcgattacg ttgttagctc aggcgattaa acaatcgatt cgtaaaagtg attatgccat
     3721 ccgactcggt ggcgatgaat tctgcatcat tcttgtcgat tcgacgccgc aaattgcagc
     3781 acaactgcct gaacgtatcg aaaaacgtct gcaacatatc gcgccgcaga aagagatcgg
     3841 cttctcttcc ggtatttacg cgatgaaaga aaacgatacg ttacatgatg cgtataaagc
     3901 ttccgatgag cgtttatatg tcaataagca gaacaaaaac agccgttcat gataaccttc
     3961 tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact gaaatggcta aaggaagcgt
     4021 gacgcatcag cggttaatcg ctttattatc acaagaaggt gctaacttcc gcgtcgttac
     4081 ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt cgtggcaccg ctttaggcca
     4141 gggagctaag gcactggtat gtaaagtcaa aggcaatggc gtaaatcaac atgttctggc
     4201 gatcctcgct gccgatcaac aggccgatct gagccaactc gccagccata taggcggatt
     4261 acgcgcttcg ttggccagcc cggcggaagt cgatgaactc acaggctgtg tcttcggcgc
     4321 gatcccccct ttcagcttcc atccaaaact caaactggtt gccgaccctc tactcttcga
     4381 acgatttgat gaaatcgcct tcaatgcagg gatgctggat aaatccgtta ttttgaaaac
     4441 cacggattat ttgcgcattg cgcaaccaga actgctcaat ttccgccgca ctgcgtaact
     4501 agcctgtccg ttcaataagc agaacggaca aaaccagcac aacagcaatg gcaaaaaacg
     4561 atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt catagcatgc ccccgggaga
     4621 tagctgtttt cacgttattg ttagcgtgca caaatggcag tttgatgaca gttcgcctga
     4681 ttttttatgc aaaaaacgta aagattttct acttccttcc tgcagcaagc gtaaagtaag
     4741 caggcttatt attttttggc aaggaaacca cgatgtttga tgtcactttg ctgatcctgc
     4801 tcgggttagc tgcgctgggc tttatcagtc ataacaccac tgtcgccgtt tcaattctgg
     4861 tgttgatcat tgtccgcgtc acaccgttaa gcaccttttt cccgtggatt gaaaaacagg
     4921 ggctaagtat cgggataatc attctgacga ttggcgttat ggcccccatc gccagcggga
     4981 cgctaccgcc ctcaaccttg attcactcgt ttctgaactg gaaatcgctg gtggcaattg
     5041 cagtaggcgt tattgtctcc tggctgggtg ggcgcggcgt gaccttaatg ggcagccagc
     5101 cgcaactggt cgccgggctg ctggtaggca ctgttttagg cgtggcgctg tttcgcggcg
     5161 taccggtcgg cccgcttatt gccgcgggtc tggtttcgct gattgtgggg aaacagtagt
     5221 taatcccgca atatatcggc ctggcgtttg ccccaggcct ttcttaaaca tagtaataaa
     5281 ggccgttgtt gaatcatacc ccagcgtatg agcaactttc tgtacagtat cgcctttcac
     5341 taatccctga agtgccataa tcagctgtaa ctgctgacgc cactggcgaa aactcaaccc
     5401 cgtctctttt actatcagtc gggcaagatt gcgttcactc atcgcaaaaa agccagccca
     5461 ttgccctaat gacccccact cgacgggccc tttcgccatc atctccacca tcgtgcggat
     5521 tttaggatga gaagaaacgg gcaggtgcag ctgttgttgt ggctgctgag ggagttcatc
     5581 aaacaggacc tgaattaatc tgcgggtcat cggctcagca cgctgagtcg tcgtcctgtt
     5641 tgccagggtt aaaatcaatt cccggcataa gggcgatatt ttcaatgtgc aacatgttgt
     5701 cggcattgtc actgcgctgg gttcgataaa cagaaaacag agttcagcgt tagctgtcac
     5761 ttgattgcta tgttccaccc cgcccggtat ccaaaccgca tattgcggcg gcaccatcca
     5821 caaagcattt tccaccgtac aggtaattgc accatgtaat gctagtatca gctgtccttt
     5881 gcggtgctga tgtacaggac tggttaactc atccgttccg gcgtgaatac aaaatgcgac
     5941 cgcggcttca tggtgtcggt cgggttcata gccattgaga ttcaacctgt gcatcatttt
     6001 gtccgaattt agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta
     6061 aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc
     6121 aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc
     6181 tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg
     6241 caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc acctttggct
     6301 gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc
     6361 tgtgcaggta tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg
     6421 gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat
     6481 ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg
     6541 gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg
     6601 ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga
     6661 agccaacaac atgcaaattt gagtacctcg cgcgccttac atactcgggg tatctggcgt
     6721 tcgccgcttg cctggcaggt cacattgttt cttgggatca actcactggt ctattacgtg
     6781 attattggct ggcttccggc gatcctcatc agtcgcggct atagcgaagc acaggcgggt
     6841 tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat ccctcttttc
     6901 ttacatcatg tgaaagatca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg
     6961 ggcgctgttg ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt
     7021 tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg ggcaagttct
     7081 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     7141 tgtgggccgc cgctgatggg taaaatacac gatgctaacg gtgactggtc cgtaccactg
     7201 atgggtgttg ccatactttc actactgatg gcgattttcg gactttgcgc cgggggagac
     7261 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcatgcgt gaaagggaga
     7321 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     7381 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     7441 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     7501 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcttgcag aactggcgca acacgagcaa
     7561 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     7621 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     7681 tagtacagca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     7741 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     7801 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     7861 aatctgcggc ggtttaggtg cttctggctg agaagaggtt gaacaaccag ccaggaccag
     7921 caagcaaggc aaagcaaaac tgataatttt catttattga tctcacacat ttatctacgc
     7981 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     8041 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     8101 atcagcattt acccgccgta tcctggagtt gtttcgtgtc agatcagatt atcgcccgcg
     8161 tctcgcaatc ccttgccaaa gaacagtcac tagaaagtct ggtccgacag cttctggaga
     8221 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     8281 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     8341 ccgtctcgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     8401 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     8461 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     8521 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgctggac
     8581 tgattgcaca atatattcaa aaagaggcgc tggttgaaca gctgcgcgaa gccaatgctg
     8641 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     8701 ttgaaaatct gacgacgctg ttttctctcg cccggcatct taaccataag ataatgatcg
     8761 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     8821 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     8881 ttggtcgtct cggcggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     8941 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     9001 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     9061 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     9121 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     9181 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttta ttacagcggt
     9241 cgtattttta gcatggtttt ttattggcgg ctatgctgcc ccgggagcat aaagatgaaa
     9301 aaaacaacga ttattatgat gggtgtggcg attattgtcg ttctcggcac tgagctggga
     9361 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     9421 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     9481 caaccacgaa gctgccaaaa ttgaagccat cgactttacc aaagccaaac agtgtgctga
     9541 tccatccgcc gactacggca ccgactatcc ccagcagaat agtcataaag aatccaccac
     9601 catctttacc tggcatgatc cacttcgcca gaataccggc aataagccca aaaataatcc
     9661 atgacagaat gcccattgtt tcctcactta tctgttttgc attagcgggt tagtcgctga
     9721 taaaaagcat agcacaacat cgggagggca agatttgtga cgagcatcac ggaggatttt
     9781 tttgcggatg gcacagaaat tgcgccatca acgatcagtg ataattacca accacaaaca
     9841 tcatgttcgt tttccgtgtc ttgacatcct ccacgccctg aatgacgagg atccctgcta
     9901 cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgggc ggcctgactg
     9961 caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagcgccgtt
    10021 tacatcggcg ttcgctgtat atccacatac ctggcatctg aattgacttt gtgacaggcg
    10081 attttctttc gctgtatgac cacagcacgc gtaacgctgg cttgtgtacg ctggcggaac
    10141 agcaagcacc tgaccgccac gccagagctg cttgtactca agctggcggc gcatttcata
    10201 ccagccctga tccagtatcg aacggtttaa acctgatttt gcccggacat tgcgccccgg
    10261 ctggctgacc gtacccgctg ctgactttga catgttgctg accttcaaat cctcaatgac
    10321 tatcattgcg tggtttttgc tgacggtcgt tgtgactttg tgaaggtagt ccctgcggat
    10381 atttgcgata cgggaatgca gtcgctgtat tttgcgtttc tgcttctgcc agttgttgct
    10441 gaatttgacc ttgcggctta actgtcgctg aagtctcgcc agcgtcttct ggtttttctg
    10501 aaaactgttt acaggctcaa agactgtgcc atctgacagc gtggcgagtt tagccacgcc
    10561 agcatccagc ccgaccattg atgctgaagg gtgaaccgga gtggatactt cactttctgt
    10621 ctgaatac
//
LOCUS       DAFATA010000131        10249 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFATA010000131 DAFATA010000000
VERSION     DAFATA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.132"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..602)
                     /gene="thrB"
                     /locus_tag="LGJ20_004202"
     CDS             complement(<1..602)
                     /gene="thrB"
                     /locus_tag="LGJ20_004202"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBK2388201.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDC"
     gene            complement(604..3066)
                     /gene="thrA"
                     /locus_tag="LGJ20_004203"
     CDS             complement(604..3066)
                     /gene="thrA"
                     /locus_tag="LGJ20_004203"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBK2388202.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(3092..3211)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(3147..3212)
                     /gene="thrL"
                     /locus_tag="LGJ20_004204"
     CDS             complement(3147..3212)
                     /gene="thrL"
                     /locus_tag="LGJ20_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBK2388203.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(3426..4112)
                     /locus_tag="LGJ20_004205"
     CDS             complement(3426..4112)
                     /locus_tag="LGJ20_004205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBK2388204.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(4512..4652)
                     /locus_tag="LGJ20_004206"
     CDS             complement(4512..4652)
                     /locus_tag="LGJ20_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388205.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            4748..5464
                     /gene="arcA"
                     /locus_tag="LGJ20_004207"
     CDS             4748..5464
                     /gene="arcA"
                     /locus_tag="LGJ20_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBK2388206.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(5524..6876)
                     /gene="creD"
                     /locus_tag="LGJ20_004208"
     CDS             complement(5524..6876)
                     /gene="creD"
                     /locus_tag="LGJ20_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBK2388207.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(6934..8358)
                     /gene="creC"
                     /locus_tag="LGJ20_004209"
     CDS             complement(6934..8358)
                     /gene="creC"
                     /locus_tag="LGJ20_004209"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBK2388208.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(8358..9047)
                     /gene="creB"
                     /locus_tag="LGJ20_004210"
     CDS             complement(8358..9047)
                     /gene="creB"
                     /locus_tag="LGJ20_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBK2388209.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(9060..9533)
                     /gene="creA"
                     /locus_tag="LGJ20_004211"
     CDS             complement(9060..9533)
                     /gene="creA"
                     /locus_tag="LGJ20_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBK2388210.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            9744..>10249
                     /locus_tag="LGJ20_004212"
     CDS             9744..>10249
                     /locus_tag="LGJ20_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2388211.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLG"
BASE COUNT         2587 a         2779 c         2408 g         2475 t
ORIGIN      
        1 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc cgtcgagact
       61 ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
      121 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
      181 tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc cagcaaacga
      241 gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
      301 caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt catcgccact
      361 ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc
      421 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
      481 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt caccgccgcc
      541 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
      601 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccggcagc
      661 tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
      721 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattaccatc
      781 cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa catagcgcaa
      841 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
      901 cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg gcagcacagg
      961 ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga gaatcaatag
     1021 cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg tataacccat
     1081 ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
     1141 ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac cagcattgag
     1201 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
     1261 tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca tcgacgaggt
     1321 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt cggcatattg
     1381 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
     1441 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg ccagttcttc
     1501 ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg agttggcaac
     1561 accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac gcttcagttg
     1621 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
     1681 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
     1741 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
     1801 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
     1861 taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta agccttcttt
     1921 cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg
     1981 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
     2041 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
     2101 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
     2161 gtcttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
     2221 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
     2281 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
     2341 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
     2401 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
     2461 aagtaccacc agttcgcctt tttcattacc ggcggtgaaa cctgccatca acaccatgtg
     2521 atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
     2581 ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg
     2641 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
     2701 cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat gcaggacatg
     2761 ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg ggaaccccgg
     2821 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
     2881 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
     2941 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
     3001 atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
     3061 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg
     3121 gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg
     3181 gtggtggtaa tggtggtgct aatgcgtttc atggatgttg tgtactctgt aatttttatc
     3241 tgtctgtgcg ctatgcctat attggttaaa gtatttagtg acctaagtca ataaaatttt
     3301 aatttactca cggcaggtaa ccagttcaga agctgctatc agacactctt tttttaatcc
     3361 acacagagac atattgcccg ttgcagtcag aatgaaaagc tgaaaaatac ttactaaggc
     3421 gttttttatt tggtgatatt tttttcaata tcatgcagca aacggtgcaa cattgccgtg
     3481 tctcgttgct ctaaaagccc caggcgttgt tgtaaccagt cgaccagttt tatgtcatct
     3541 gccactgcca gagtcgtcag caatgccatg actcgttcgc gtaaagcttg cagttgatgt
     3601 tggtctgccg ttgtgtcact tttcgccggt tgttgtatta atgttgctaa ttgatagcaa
     3661 tagaccatca ccgcctgccc cagattgagc gaaggataat ccgccaccat cggcacacca
     3721 gtaagaacgt cagccaacgc taactcttcg ttagtcagcc cggaatcttc gcgaccaaac
     3781 accagcgcgg catggctcat ccatgaagat ttttcctcta acagcggcac cagttctact
     3841 ggcgtggcgt agtaatgata tttcgcccga ctgcgcgcag tggtggcgac agtgaaatcg
     3901 acatcgtgta acgattcagc cagcgtcggg aaaactttaa tattatcaat aatatcacca
     3961 gatccatgtg cgacccagcg ggtggctggc tccaggtgcg cttgactatc gacaatccgc
     4021 agttcgctaa aacccatcgt tttcattgcc cgcgccgctg ccccaatatt ttctgctctg
     4081 gcgggtgcga ccagaataat cgttatacgc atattgccac tcttcttgac caaataaccg
     4141 cgaacagggt gatcactgtc aacttattac gcggtgcgaa tttacaaatt cttaacgtaa
     4201 gtcgcagaaa aagcccttta cttagcttaa aaaaggctaa actatttcct gactgtacta
     4261 acggttgaat tgttaaaaaa tgctacatat ccttctgttt acttaggata attttataaa
     4321 aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca actagttgga
     4381 ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc aaactgttaa
     4441 cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg tgtccggccc
     4501 ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt tgacactgtc
     4561 gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt
     4621 gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta atttgcagca
     4681 tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg caatttaggt
     4741 agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa cacgcaacac
     4801 gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag atggcgcgga
     4861 aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata tcaatctgcc
     4921 gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat
     4981 gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga
     5041 tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac gcaacctgct
     5101 gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa
     5161 gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg atggcgagca
     5221 gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa acccaggcaa
     5281 aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga aaccacacga
     5341 ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta cgccggatac
     5401 gccggaaatc atcgccacca tccacggtga aggttatcgc ttctgtggtg atctggaaga
     5461 ttaatcggct ttaccaccgt caaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     5521 accttatttc cagatacgta attgatcgtc cattgtaact tctttactgg ctttcatttt
     5581 cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc cggccagcgc
     5641 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     5701 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
     5761 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
     5821 tgccacggta aaaccggtat gttcagaaag cgccagcaag agcaaataaa acatcaccaa
     5881 tgaaagcccc accagcaaat attgcattgg gtgtaagcgt tgcgcggtga gcgtttcaaa
     5941 aacaaagaac gccataaaag tcagcgcaat cagcagaatg gcatacttag tcgcccggtc
     6001 agttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
     6061 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttatttgcaa accagctgct
     6121 ttgccattgc gcctgaaaac ctgactcgct aacttcccgt ttggctggca aaaaatcacc
     6181 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
     6241 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
     6301 cttgcgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
     6361 ggtaccgggt tcgatggtca gcgctgttcc gttaacttca ggcgctttca ccacaccaat
     6421 accacgcgca tccccgacgc taatcacaat aaatggcttg cctaaggtga tatttggtgc
     6481 gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     6541 gacctgaccg gtataaatcc ctatcttgcg ttcttcaacg ttctgattgc catcaaccat
     6601 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcgtc
     6661 ttcctgcacc gtataaagct cggtcacagg aatagcgatg agcggcccaa cgagtttttg
     6721 cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
     6781 gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacag
     6841 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     6901 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     6961 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
     7021 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
     7081 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
     7141 atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg ttatacgacc
     7201 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gatccagtaa
     7261 cgccggttca gcagcaacat taacctcggt gggcgtaaca tgtagagtga tgtttttttc
     7321 tgccaactgc acggtgcgcg cttcgctgac gcggcggaat aatgccgcca catcaacagc
     7381 agtcaaaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag
     7441 tgcctgcatt cgcgcatttt gcgtcagaat gttgtcagta aaacgagcca ctacttcagg
     7501 cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
     7561 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     7621 catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat cgggaagagg
     7681 aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tggagcggtt
     7741 gatccaccaa accatgcctg cgccaatcac cagtgcgatc cccaacaaaa tggcgctggc
     7801 ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt tcggtttgcc
     7861 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
     7921 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
     7981 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
     8041 gaacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
     8101 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
     8161 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
     8221 tgcggtatcg attaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
     8281 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
     8341 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
     8401 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
     8461 tcaacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
     8521 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
     8581 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
     8641 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
     8701 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
     8761 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
     8821 aacgccggat ggagcgccag taactggcgg cataattcaa agccgctgat atcaggcaaa
     8881 ccaacgtcga gaatcatgac gtcgggaacc agctggcgag ctttatccag caccggcaag
     8941 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
     9001 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
     9061 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
     9121 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tagcatcata aaagcgcacg
     9181 acctgtaacg acttaaagac cagggacgta cgttttttga atactacctc gccctgagct
     9241 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatggcc
     9301 gcatcggagg tatcttccgc caggcccaat ccccctttaa tacccccggt tttcgcccgg
     9361 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
     9421 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
     9481 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
     9541 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
     9601 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
     9661 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
     9721 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
     9781 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
     9841 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
     9901 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
     9961 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    10021 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    10081 gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc
    10141 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    10201 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcgg
//
LOCUS       DAFATA010000132        10222 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFATA010000132 DAFATA010000000
VERSION     DAFATA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.133"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..998
                     /locus_tag="LGJ20_004213"
     CDS             <1..998
                     /locus_tag="LGJ20_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2388212.1"
                     /translation="HKLISTHPNGYMTPQEFWELVVTCLCLRGNFYAYKVKAFGEVAE
                     LLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLSQEDIWHVRTLTLDGLVGLNPIAYA
                     REAISLAAATEEHGARLFSNGAVTSGVLRTEQTLSDQAYERLKKDFEERHTGLGNAHR
                     PMILEMGLDWKSMALNAEDSQFLETRKFQLEEICRLFRVPLHMVQNTDRATFNNIEEL
                     GLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYYAKFNAGALLRGDMKSRFEAYATGI
                     NWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTKPSDGSKAGKQKDNANADETTS"
     gene            976..1626
                     /locus_tag="LGJ20_004214"
     CDS             976..1626
                     /locus_tag="LGJ20_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBK2388213.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            1641..2846
                     /locus_tag="LGJ20_004215"
     CDS             1641..2846
                     /locus_tag="LGJ20_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK2388214.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATGVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            2896..3096
                     /locus_tag="LGJ20_004216"
     CDS             2896..3096
                     /locus_tag="LGJ20_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388215.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGSSAKSSKAAKEKKATTSKEG"
     gene            3099..3422
                     /locus_tag="LGJ20_004217"
     CDS             3099..3422
                     /locus_tag="LGJ20_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK2388216.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            3419..3829
                     /locus_tag="LGJ20_004218"
     CDS             3419..3829
                     /locus_tag="LGJ20_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK2388217.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VAFRTWAKVVQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVWGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGNNDDFLFAR"
     gene            3804..4310
                     /locus_tag="LGJ20_004219"
     CDS             3804..4310
                     /locus_tag="LGJ20_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388218.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNHSWTRYFLARELRKS
                     LKPERRHR"
     gene            4307..4867
                     /locus_tag="LGJ20_004220"
     CDS             4307..4867
                     /locus_tag="LGJ20_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388219.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDELNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            4876..5046
                     /locus_tag="LGJ20_004221"
     CDS             4876..5046
                     /locus_tag="LGJ20_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HBK2388220.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            5030..6526
                     /locus_tag="LGJ20_004222"
     CDS             5030..6526
                     /locus_tag="LGJ20_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBK2388221.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPTLPFTASSSA
                     GVVTLTARHKGLCGNEVPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKTGTLSELVNAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            6526..6882
                     /locus_tag="LGJ20_004223"
     CDS             6526..6882
                     /locus_tag="LGJ20_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBK2388222.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVGKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            6882..7151
                     /locus_tag="LGJ20_004224"
     CDS             6882..7151
                     /locus_tag="LGJ20_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBK2388223.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQNSVDQMSPADLNAAAWLVAGFFLQA"
     gene            7293..9128
                     /locus_tag="LGJ20_004225"
     CDS             7293..9128
                     /locus_tag="LGJ20_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388224.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKRFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFKQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            9189..>10222
                     /locus_tag="LGJ20_004226"
     CDS             9189..>10222
                     /locus_tag="LGJ20_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HBK2388225.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTCWPSVTHPALNNAPAVKN
                     TVDLPTWEELT"
BASE COUNT         2422 a         2554 c         3069 g         2177 t
ORIGIN      
        1 tgcataagct gatctccacg catcccaatg gctatatgac gccgcaggag ttctgggagc
       61 tggtggtcac ctgtctgtgc ctgcggggaa acttttacgc ctacaaagtg aaagcatttg
      121 gcgaagtggc tgaactgctg cccgtcgatc ccggctgtgt ggtaccgaag cttaacagta
      181 gctgggagcc ggtctatcag gtcacattcc cggatggctc cacggatgta ctgagccagg
      241 aggatatctg gcatgtgcgc acgctgacgc tggacggact ggtggggctg aatcccatcg
      301 cctatgcccg cgaggcaata tcgctggcgg cagcgaccga agagcacggg gccagactgt
      361 tcagcaatgg cgcggtgacg tcgggtgtgt tgcgtacaga gcagacgctg tcagatcagg
      421 cttatgagcg cctgaagaaa gattttgagg agcgtcacac cgggcttggc aatgctcacc
      481 gcccgatgat ccttgagatg gggctggact ggaagtcgat ggcgctgaac gccgaggaca
      541 gccagttcct ggaaacccgc aagtttcagc ttgaagaaat ctgtcgtctg ttccgggtgc
      601 cgttgcacat ggtgcagaac accgatcgcg ccaccttcaa caatatcgaa gagctggggc
      661 tgggatttat caactattca ctggtgccgt atctgacccg catcgaacag cggatcaaca
      721 ccggactggt acgaaaaagt aagcagggcg tttattacgc caaatttaac gccggggcgt
      781 tactgcgcgg ggatatgaag tcccgttttg aagcctacgc caccgggatc aactggggaa
      841 tttactctcc caatgactgc cgcgacctgg aagatatgaa tccgcgtccc ggtggtgatg
      901 tctatctcac accgatgaac atgaccacga aaccctccga tggcagtaaa gccggtaagc
      961 agaaggataa cgccaatgca gacgaaacaa cgtcttgatg taccgctgag tctgaaatct
     1021 gtcagtgact ccggtgagtt tgaagggtat ggctccgtct ttggtgtaaa ggacagccac
     1081 gatgatgtgg tgatgtccgg ggcatttgct gcttccctgc gggcgtggag tgacagaaaa
     1141 gcgttacctg cgctgctctg gcagcaccgc atggatgaac ccatcggtgt ttacaccgaa
     1201 atgaaggaag acgatgtcgg gctttacgtc agggggcggt tgctcattga tgatgatccc
     1261 ctggcaaaac gcgcacatgc acacatgaag gccggttcgt taaccggcct ttctattggg
     1321 tacgtcctga aggactggga atacgaccgg acgaaagaag cctttctgct gaaagaaatc
     1381 gacctctggg aagtcagtct ggtgacgttt ccgtctaacg acgaggcgcg gatcagcgac
     1441 gtcaagaacg cgctggcccg cggggaaatc cccgaacaga aaaaaatcga aagagtcctg
     1501 cgtgatgtcg gactctcccg tacccaggcc aaagcattca tggccggggg ctatggcgca
     1561 ctgtccctgc gcgacgctga ggatgtgggc tctgcactga atgcactgaa aaatctgaac
     1621 ttctaatcag gagaaatacg atggcggtag atattaaaga tgtcgaacag gtcgcgcagg
     1681 agctgcagca gaagtttgac gacttcaaag caaagaacga caagcgcgtg gatgcgattg
     1741 agcaggaaaa aggcaaactt gccgggcagg tggaaaccct gaacgggaaa ctcagcgagc
     1801 tggaaaatct caaaagcgat cttgaaaaag agctgcttga gctgaaacgt ccggcaggtg
     1861 gcgcgcaaaa taaactggcc accgagcata aagaagcgtt tgtgggcttt ttgcgtaaag
     1921 gccgtgaaga tggtctgcgc gatctggagc gtaaggcatt gcaggtgggc accgatgaag
     1981 acggtggcta tgccgtgccg gaagcgctgg atcgcaacat tctcaccctg ctgaaagatg
     2041 aagtggtgat gcgccaggaa gccacggtga tcaccgttgg cggttccgac tacaaaaaac
     2101 tggtgaatct gggcggtacg gcttccggat gggtggggga aacggatacg cgatcccaga
     2161 ctgccacctc cagactggag ctgattgaac ctctcatggg ggaaatctac ggcaacccgc
     2221 aggctaccca gaaaatgctg gacgatgcct tcttcaacgt ggaggcctgg atcaacagcg
     2281 agctggcaac cgaatttgcc gaacaggaag aaattgcctt tacctccggc gatggcacca
     2341 agaagccgaa agggttcctg gcgtatgaat ccacggatga aaccgataag gtccgggcgt
     2401 tcggcaaact tcagcatatt gtatccggcg aagcgacggg ggtgaccgca gacgccatta
     2461 tcaaactgat ttacacgctg cgaaaggcac accgcactgg cgcgaagttc atgatgaaca
     2521 acaacagcct gtttgccatc cgtttgctga aagacaccga gggtaactat ctgtggcgtc
     2581 cggggctgga actggggcag ccgtcctctc tggcgggtta cggtatcgct gaaaacgaac
     2641 agatgccgga tatcgccgct gatgcgaaag ccattgcatt tggtaacttc aaacggggtt
     2701 acaccatcgt tgaccgtatc ggcacccgca ttctgcgtga cccgtacacc aataaaccgt
     2761 ttgtcggttt ttataccacc aagcgcaccg gcgggatgct ggtcgattcg caggccatca
     2821 aactgctgaa gattgcagcg gcgtaatcat tcaggggcgc ggaaccgcgc cccctgttct
     2881 gacgggtgaa gaatcatgat cctgaaacaa gatctgaaat ggtcaccgga cggtatgcgt
     2941 gttgaggtca ttcgggccgg tgagtatgac gacggggcgc ttcctgcccg ggtgcaggag
     3001 attgcacttc aggccgggtt agcagagcgc ggaagcagtg caaaaagcag taaagcggca
     3061 aaagagaaaa aagccacgac cagtaaagag ggctgagtat gcttctgaca atggaagaga
     3121 ttaaagccca actccggctg gatgaggatt tcgatgctga tgaccgccat ctgcaactgc
     3181 tggcctgtgc ggcgcaaaag cggacggaaa cgtatctgaa ccggaagctc tatgcaccgg
     3241 atgaaaccat tccggacagc gatccggacg ggctgcacct gccggatgat attcgtctgg
     3301 ggatgctgat gcttatcagc catttttacg aaaaccgctc gtcggttacg gaagtggaga
     3361 aactcgacat gccgcagagt tttggctggc ttgtcggccc gtacaggtac tttccgcaat
     3421 gaaaattcgt caggcgcaga ccagcgcaac ctacattctg ccggaccccg gtgaactgaa
     3481 taaacgcgtc ctgatccgcc agcgggtgga tatgcccgcg gataactttg gcgtggagcc
     3541 tcaatacccg gttgcgttcc ggacatgggc gaaggttgtc cagaccagtg ccaccacctg
     3601 gcaggaaacc gcgcagaccg gtgacgccat cacccattac atcaccattc gttaccgccg
     3661 ggggatcacc gctgattatg aggtggtctg gggtgacagt gtgtaccggg tgaaacgtca
     3721 gcgcgatctg aacggggcgc ggcgctttct gctgctggag tgtacggagc tgggcgaatg
     3781 taggcagagt cacggaggca acaatgacga cttccttttt gcacgttgat tttcagcagc
     3841 ccgcggagat gcgctttaac cgcgcccgtg tccggcgggc gtttgtcacg attggtcagc
     3901 gtcatatgcg tgatgcccgt cggctggtga tgcgccgtgc gcggtcggca ccgggtgaaa
     3961 accccggtta tcagaccgga cgcctggctc gttcgattgg ttacatggta cccagagcca
     4021 gtaaacatcg ccctggtttt atggcacgta tagcccctaa ccagcgtaat ggagagggaa
     4081 accgccgtat caccggtgat ttttatccgg cttttttgtt ctatggcgtg aggcgaggtg
     4141 caaagcgtcg tcgcagccat catcgtggtg catccggtgg cagcggctgg cgactggctc
     4201 cacgtaataa cttcatggtg gaaacgcttg aaaagaacca cagctggaca cgctattttc
     4261 tggcgcggga attgcgtaaa tcactgaagc cggagcgacg acacagatga aactgacgcc
     4321 tgttattgct gcgctgcgtg cccgctgccc gtattttgaa aaccgggtgg caggcgcggc
     4381 acagttcaaa aatctgccgg aggtcggaaa gctgagactc ccggcggcgt atgtggtacc
     4441 gggggatgat tctccgggag aaaacaaaag ccagaccgac tactggcagg agctgaaaga
     4501 gggcttctcc gtggttgtca tactgagtaa cgggcgtgat gagcgcggtc agtttgcttc
     4561 gtatgatgtg gtggacgatg tccggcagat gctctttaag gccctgctgg gctggaaccc
     4621 ggaagcgtgc ggtaacccga ttacctatga cggcggcacg ctgctggatc tgaatcgtca
     4681 tgagctgatt tatcagttcg atttttcggt catcagcgag ctgaccgaag acgatacccg
     4741 ccagcaggat gagctgaaca gtctggatga actgcgaacg ctggcgattg atgttgatta
     4801 tctcgatccc ggtaacgggc ctgacggcga tatcgaacat cacaccgaaa taacccttcc
     4861 ttcctgagga tcctcatgtt tgtcaaacct gttaaagggc ggtcagtgcc tgaccctgcc
     4921 cgcggcgacc ttttgcccgc cgaagggcga aatgttgacg agaacaacta ctggctgcgc
     4981 cgtgaagcag cgggtgatat ccggcgcgtg aataaaaagg tgaacaccga tgacgataag
     5041 ctttaacacc attccgtcga atacgctggt tccgttgttt tatgcggaaa tggataacca
     5101 ggcggcgaat actgcacagg acagcggagc atcgctgctg attggtcatg ccaataacgg
     5161 tgcagagatt gttgccaaca gtctggtact gatgccgtcg gcagactatg cacgccagat
     5221 ttgtggtgcg ggaagtcagc tggcgcgtat ggtcgaggct tatcgccaga ctgacccgtt
     5281 tggcgagctg tatgtgattg ccgttcctga atccacaggc gcggcggcaa cggttacgct
     5341 gacggtgacc ggggaagcaa ccgaaagcgg cacggtgaat gtctatgtgg gacgtacccg
     5401 cgtgcaggct ccggtgacca acggcgataa cgtcacgacg attgccagca gtatccagga
     5461 tgccatcaat gccgttccga ctctgccgtt tacagcttca tcttcggctg gtgttgtcac
     5521 gctgaccgcg cgccataagg ggctttgcgg gaatgaagtt cctgtcagcc tcaattacta
     5581 cggcttcggt gggggtgaag tgctgccagc gggcgtacag attgccgtgg cgacggggac
     5641 cgccggaacg ggcgctccgg ttctcaccgg cgcggtggct gcaatggcgg atgagccgtt
     5701 tgattatatc ggtctgccgt tcaacgacac ggcctccgtt aacacgctgg tgaccgagat
     5761 gaacgatacc agcggtcgct ggagctatgc gcgtcagctg tatggtcatg tgtatacggc
     5821 aaagaccggc acactgtcag aactggtgaa cgcaggtgac cagtttaacc agcagcacat
     5881 caccctggcg gggtacgaaa aagagaccca gacgcctgcc gacgagctgg cggcaagccg
     5941 taccgcccgc gcagcggtgt ttatccgcaa cgatccggca cgtcccacgc agaccggtga
     6001 gctggtgggg atgctgcctg cgccgaaggg gaaacggttc acgatgaccg aacaacagac
     6061 cctgctgtct catggcgtgg caacggcgta tgtcgaaagc ggggtgctgc gcattcagcg
     6121 tgatgtcacc acgtacagga aaaatgctta cggggttgcg gataacagct acctcgacag
     6181 cgagacgctg cataccagtg cgtatgtact gcgcaaactg aaatccgtca ttaccagtaa
     6241 gtacgggcgt cacaagcttg ccagtgacgg tacccgcttt ggtcccggtc aggcgattgt
     6301 caccccggcg gtgatcaaag gggaactgct ggcaacctac cgtcagctcg agcgtgcggg
     6361 gatcgtggaa aactacgaac tgtttaagca gtacctggtt gtggagcgtg atgccagcga
     6421 tccgaaccgc ctgaacacgc tgttcccgcc tgactatgtt aaccagttgc gtgtctttgc
     6481 cgtggttaac cagttccgtc ttcagtattc agaggagtcc gcataatggc ccgtatcggg
     6541 ggaacctgtt atttcaaaat tgacggtcag cagctatcgc tgaccggcgg cattgaggtg
     6601 cccatgaaca ggacggtcaa tgatgacatc atcggcctgg acggttcagt ggaccgcaag
     6661 gaaactcacc gtgcgcctta tgttaaaggg accttcaagg tgccgaagaa ttttccggtg
     6721 ggcaaaatca cctcgtctga tgagatgacc atcactgccg agctggcgaa cggtcaggtc
     6781 tatgtactgt cgtctgcctg gctgcacggc gaagcgaacc ataatgccga agaagggacg
     6841 gttgatcttg agttccacgg tgaagaaggg gattaccagt aatgaaagag cttgagttaa
     6901 agaaaccgat taccgctcat ggcgagacac tctccgtact ggagtttgat gagcccaccg
     6961 ggaaagatgt ccgcgagctg gggtatccct accagatgaa tcaggatgag tccgtcagac
     7021 ttctggcgca tgtggtatcg aaatacattg tgcggctggc gaaagtgccg caaaactctg
     7081 tcgaccagat gtctccggca gacctgaatg ctgcggcgtg gcttgtggct ggttttttcc
     7141 tccaggcctg acggctgaat acctcactga tcgcttcttt gactgcgcca gctactggcg
     7201 cattaatccc ttcgaattgc tgaatatgcc gatcagtgaa attcccttgc tggtcagtca
     7261 ggcaaacagg atagagcagg agaaacgcac acatggctga atttgagctt aaggcgttga
     7321 tcaccggtgt cgacaggctt tctcccgcgc tgtcgaaaat gcaaaagaaa atccggggat
     7381 ttaaacgcca ggcggaagaa gcgtcacagg gtgggctggc gcttggtggc ggactggctg
     7441 cgggtctgac gctttccctg aaatcttatg ctgatcagga aaacgccgcc accgggctga
     7501 aagtcgccat gatggatgcg aacggcgagg ttggaaagcg ctttcaggac atcaataaac
     7561 tggctattgg cctgggtaac cagctacccg gtacaacggc tgatttccag aacatgatgc
     7621 agatgctggt gcgtcagggg atcccggcag aaaacattct tggtggtgtg ggtaaagcga
     7681 cagcttatct tgcggtacaa ctgaaaaaaa caccggaagc ggctgctgag tttgctgcaa
     7741 agatgcagga tgctaccgga acggcgtcag aagacatgat ggggctgttc gacactatcc
     7801 agaaggcgtt ttatctgggc gttgacgata ccaacatgtt gtccttcttc actaaaacca
     7861 gttctgttct gaagatggtg aataaggacg gacttcaggc tgcacagagc cttgccccta
     7921 tcagcgtcat gatggatcag atggggatga acggagagtc ggcaggtaat gccctgcgaa
     7981 aagttatcca gtccggatta agcgttaaga aaatcaggga cgtcaataaa gtcatggccc
     8041 gccagaaact cggagtgcag ctcgatttta ctgacggcaa agggagtttt ggcggtcttg
     8101 ataacatgtt taagcaactg gcaaagctgc gaaaactgac cgacgttaag cgaacaggtg
     8161 tacttaaggc aatatttggt gatgatgccg aaacccttca ggtggtcaat gctctgatcg
     8221 ataaaggaaa ggatggttac gatcagatcc agcagaagat gaataaacag gccagcctga
     8281 ataaacgtgt tcaggcacag cttggtacgc tgtccaacct gtgggaggca atgacgggga
     8341 ccgcaactaa cggtcttgca gctattggcg gcgcattttc tggtgacgcc aaaaatatca
     8401 cgcaatggct gggggagtta ggggaaaaat tcacgaagtt tgcggatgaa aatccccggg
     8461 ttattcgcgg cgtcgtcggg cttgctgccg gtcttgcgat tctgaaactg ggattgatgg
     8521 gcgttggcgg tgccatcagt attgtcagca ggatcatgtc gatgacgccg attggaatga
     8581 ttgcgacggc gatagccctg gctgcgggat taattatcac taactgggat gttgtcggac
     8641 cttatttcaa gaagctctgg gaaaccattg gtccttattt tgaggctggc tgggaacttc
     8701 tgaagaaggt ttttgcctgg tcgccgctgg ggatggtaat caataactgg ggaccggttg
     8761 ttaagtggtt tcaggatatg tgggacaagc tgaagccaat tattgagtgg tttaccgaca
     8821 gttccggtga cacggtcgat gccattaact ctgcgcagtg gggcgcgggt gcttatgatg
     8881 cttatgggac gggaataccg gcacggggat acacacctta tcaggcggtg gatccggctc
     8941 agtcaaacaa cgcctccgat gccacaggcc cgaatccctt catgattaac aaagcttctg
     9001 cgccaaaagt tgatggtgag atcaaggtct cttttgtgaa ttcgcctccg ggtatgcggg
     9061 ttatggaaac gcgatccagc ggttttgatg tcagccatga tgttggctat acgcgctttg
     9121 gcaggtaatg aaaaattaat ctgttaatga gtcccactcc ggtgggattt tttatgtacg
     9181 gagtttatat gacgtggaaa gacagacttc aggacgcgtc atttcgcggt gtgccattta
     9241 aggttgaaga agaaagtgcg ggaaccggtc gtcgtgtgga aacgcacgaa tacccgaacc
     9301 gcgacaagcc ctataccgaa gacctgggga aaatcacttt ccgcccgtcc atcacagctt
     9361 atgtggtggg agatgactgc tttgaccagc gcgatcgcct gattgacgcg ctgaataaac
     9421 ccggtcccgg cacgcttgtc catccgactt acggtgagct gaaagtctgt gttgacgggg
     9481 aagttcgggt cagcacatcg aagagtgaag ggcgtattgt ccgctttgac ctgaagtttg
     9541 tcgaagcggg agaactctct taccccactt caggtgcggc gacggcgcag acgctgatgt
     9601 catcctgttc tgcactggat gactgcatca gtgacagctt cagtggtttc agtatcgatg
     9661 gcgtggcaga ttttgtgcag aacgacgtcg tcggtaatgc cagcacaatg cttgggtatg
     9721 tttctgatgc gatgaaagtg gtggattctg ccgtatcgga tgccgccagg cttttgcagg
     9781 gggatatctc ggtacttctg ccgccgccat cgtcaggcaa aaatttcgtt gagcaggtgc
     9841 agaaaatgtg gcgtaccggg aaacgccttt atggtaacgc cagcgacctg gtcaccatga
     9901 tcaaaacgct ttccggtgtc agcctcggca gcgatctgca accgcgcggc gtctggaaaa
     9961 cggacagtaa aaccaccgcc acggcgacgc agcagcgtaa cgtggttgcc agcacccttc
    10021 gtacgaccgc aatcagcgaa gcggcgtatg ccgtcacacg attgcctgcg cccacaactt
    10081 ccgcggtgat gcagaatgcc acagtggggc agtcaacaac acccgcgcag agcacttgct
    10141 ggccttccgt cacgcatccg gcactgaaca atgcaccggc ggtgaaaaac acggttgacc
    10201 tgccaacgtg ggaagaactg ac
//
LOCUS       DAFATA010000133        10207 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFATA010000133 DAFATA010000000
VERSION     DAFATA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.134"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            49..816
                     /locus_tag="LGJ20_004227"
     CDS             49..816
                     /locus_tag="LGJ20_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388226.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            813..1070
                     /locus_tag="LGJ20_004228"
     CDS             813..1070
                     /locus_tag="LGJ20_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBK2388227.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            1135..1995
                     /locus_tag="LGJ20_004229"
     CDS             1135..1995
                     /locus_tag="LGJ20_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBK2388228.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            2063..3241
                     /locus_tag="LGJ20_004230"
     CDS             2063..3241
                     /locus_tag="LGJ20_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388229.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(3254..3808)
                     /gene="kptA"
                     /locus_tag="LGJ20_004231"
     CDS             complement(3254..3808)
                     /gene="kptA"
                     /locus_tag="LGJ20_004231"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBK2388230.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            4058..4741
                     /locus_tag="LGJ20_004232"
     CDS             4058..4741
                     /locus_tag="LGJ20_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388231.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAITTNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            4738..5199
                     /locus_tag="LGJ20_004233"
     CDS             4738..5199
                     /locus_tag="LGJ20_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBK2388232.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            5212..6384
                     /gene="iadA"
                     /locus_tag="LGJ20_004234"
     CDS             5212..6384
                     /gene="iadA"
                     /locus_tag="LGJ20_004234"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK2388233.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            6449..7360
                     /gene="hypT"
                     /locus_tag="LGJ20_004235"
                     /gene_synonym="qseD"
     CDS             6449..7360
                     /gene="hypT"
                     /locus_tag="LGJ20_004235"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBK2388234.1"
                     /translation="MDDSGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(7353..7745)
                     /gene="iraD"
                     /locus_tag="LGJ20_004236"
     CDS             complement(7353..7745)
                     /gene="iraD"
                     /locus_tag="LGJ20_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBK2388235.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(7742..7825)
                     /gene="idlP"
                     /locus_tag="LGJ20_004237"
     CDS             complement(7742..7825)
                     /gene="idlP"
                     /locus_tag="LGJ20_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBK2388236.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            8418..9248
                     /locus_tag="LGJ20_004238"
     CDS             8418..9248
                     /locus_tag="LGJ20_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBK2388237.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALFNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(9389..10162)
                     /gene="uxuR"
                     /locus_tag="LGJ20_004239"
     CDS             complement(9389..10162)
                     /gene="uxuR"
                     /locus_tag="LGJ20_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBK2388238.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
BASE COUNT         2475 a         2541 c         2473 g         2718 t
ORIGIN      
        1 ctcagtaccc gtgtcgcggc ctttattgag atgctgtaag gagtggcagt gacatattcg
       61 attggcattg attctggttc taccgccacc aaagggatct tactggcaga cggcgtgatt
      121 acgcgccgtt tcctcgttcc aacacccttt cgtccggcaa cagcaattac tgaagcctgg
      181 gaaactctgc gcgaagggtt agagacaacg ccgtttctga cgctcaccgg ctatggacgg
      241 caactggtgg attttgccga taaacaagtg acggaaatct cctgtcatgg gctgggcgca
      301 cggtttcttg cgccagcaac gcgcgcggta atcgacatcg gtggtcagga cagcaaagtg
      361 attcagcttg atgatgacgg taacctgtgc gatttcctga tgaatgacaa atgcgcggcg
      421 ggcaccgggc gtttcctgga ggtgatctcg cgcacgcttg gcaccagcgt cgagcaactc
      481 gacagcatta ccgaaaatgt cacgccgcac gccatcacga gtatgtgcac agtgtttgcc
      541 gaatcagaag tgatcagcct gcgctcagcg ggcgtcgcgc cagaagcgat tctcgccgga
      601 gtgattaacg cgatggcgcg gaggagtgcc aatttcattg ctcgtctctc ctgtgaagcg
      661 ccgattctgt ttactggtgg cgttagtcat tgccagacgt ttgcccggat gctggaatct
      721 catctgggaa tgccggtaaa tacccatcct gatgcgcaat ttgctggcgc aattggcgcg
      781 gcggtaattg gtcaacgagt gaggacacgc cgatgaaaga gtttttattt ttatttcact
      841 ccacggtcgg cgtcatacaa acccgcaagg cgttgcaggc agcgggcatg acttttcgcg
      901 tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg ctgacctgtc
      961 cccccggcga ggaaatacaa tgggtgatcc ctgggctgac tgagtcaatt tattgtcagc
     1021 aggatggtgt ctggcgctgc attgcacatt accgggtttc tccgcgttaa ggattcatta
     1081 aggtcgctac gtatacttct cgatatctta ttgatttgcc ttaaagggat taccgtgaca
     1141 aaaagtattt cgttaagtaa acgcatctcc gttattgtga ttctttttgc cattgtggca
     1201 gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca atcatgcagc atcatttcag
     1261 aattaccacg ccaccattga cggcaaagaa attgcaggca taacgaataa tatctcttcg
     1321 ctgacctggt cggcacaaag caatactctt tttagcacta tcaacaaacc tgccgccatt
     1381 gttgaaatga ccaccaatgg tgattttatc cgcacaattc ctctggattt tgttaaggat
     1441 ctcgagacga tcgaatacat tggcgataat cagtttgtca ttagcgatga gcgcgattac
     1501 gccatttatg tgatttcact gaccccaaac tcggaagtaa aaatcctcaa aaaaataaaa
     1561 atcccgctgc aagactcccc aaccaattgc ggttttgaag ggttggctta ttctcgccag
     1621 gatcacactt tctggttttt taaagagaaa aatccgattg aggtctacaa agttaacggg
     1681 ctgttaagta gcaatgaatt acacatcagc aaagacgagg cactacaacg gcaatttact
     1741 ttagatgatg tgtccggcgc agaatttaac cagcaaaaaa atacgcttct ggtgctgtct
     1801 catgaatctc gcgcactcca ggaagtaacc ctggttggcg aggttatcgg tgaaatatca
     1861 ctaaccaaag gtagtcgagg tttgtcgcat aacatcaaac aggccgaagg gattgcgatg
     1921 gatgcctctg gtaatattta tatcgttagc gaaccaaatc gtttttatcg ctttacccct
     1981 aaatcatcgc attaacaggc tggcatattt cacctttttg ttattaactt gttaacaaca
     2041 aaaatgacat acaaggttaa acatgccttc gtcctcgcat cccgtagaac gcttttcttt
     2101 cagcaccgcg cttttcggga tgctggttct gaccctaggt atgggattag gtcgcttcct
     2161 ttatacgcct atgttgcccg tcatgatggc ggaaggggcg ttttcattta gccagctctc
     2221 gtggattgcc agcggcaact atgcggggta tctggctggc agtctgctat tttcgtttgg
     2281 cgcatttcac cagccgtcgc gcctacgccc atttctgttg gtttctgccc tggcgagcgg
     2341 gttgttgatc ctcgcaatgg catggttgcc gccatttatt ctggtgttat tgattcgctt
     2401 cctggcgggt gtcgccagcg ccggtatgct gatttttggt tcgacgctga ttatgcagca
     2461 cacgcgccat ccttttgtgc tggcagcttt gttttctggc gttggcattg gcattgcact
     2521 gggcaacgaa tatgttctgg caggcctgca ttttgcctta tcctcgcaaa cgttatggca
     2581 aggcgccggg gcgctttctg gcatgatgct gattgcactt acgcttttaa tgccctcgaa
     2641 aaaacacgcc atcgcaccaa tgccattggc aaaaacggag caacagataa tgagctggtg
     2701 gttactggct attctgtacg gccttgcggg atttggttat atcatcgtcg cgacttacct
     2761 gccgctcatg gcgaaagatg caggctcacc attgttaacc gcccatctct ggacgttagt
     2821 cggcttatca attgtgccag gctgctttgg ctggctatgg gcagcaaaac gttggggggc
     2881 tttgccttgc ctgacggcga atttgctggt gcaggctatc agtgtgctgc ttactctcgc
     2941 cagcgactct cctctcttgc ttatcattag tagtatcggt tttggcggta cctttatggg
     3001 cacgacatct ctggtgatga ctatcgcccg ccaactgagc gttccgggaa atctcaatct
     3061 tttaggcttt gtgacgctca tttacggcat tggacaaatc ctcggcccgg cgctgaccag
     3121 tatgctcggc aatggaacat cggcgcttgc cggcgccact ctctgcggcg cggcggcgtt
     3181 gtttattgct gcgcttatca gcacagtaca attatttaag ctgcaagtgg tcacttcatg
     3241 aggagtgttt cccttaccac tcaagaaact caacagcaac cgttgatgtc agccagaccc
     3301 cgttttccgc ttgccagaaa gggatgcctc gtttcgccat ttcctgtgct ttgacggtta
     3361 agataaccgg cgatccgtgt cttgctccca ctttgcgtgc cgttgcttca tcagcagaga
     3421 gatgtacgta atgacgctcc cctgcaatca gcccctgttt ttttatctcg tcaaggaaac
     3481 ggcttgccgt accgtggtag agaaactgtg gcggtgtttt ttcggcaaag gaaatggcga
     3541 cctgcgaagt cgaatgcccc tgaaccgcgc ggatgcacct accatcactg gaataactaa
     3601 aacgtttttt atcactggta gcgaccacgg tatccagcaa tgcacgggtt aaccgcttgc
     3661 ccgctttttg ggcacaaagt atgagcttat cgatatccgc ccatccttca cggtccagta
     3721 cgatacctat cgcctccggt ttgtgtcgga ggacaaaact taaaaacttg ctcgtatccg
     3781 ccagctcttt ttcgttgtac tttgccatct cgttttcgtc cctggatttt ctgtttttat
     3841 ttactgatgt cattgtcact gggtttatga attttctgca actcaatcct gcgcagagcc
     3901 ttttccacac agcataattt ttccgctatg caaaaaacgc atagccagaa acatacttgc
     3961 attttcccca tacccacatg gctgataagg tttttcagct ccaggaattt tcctggctca
     4021 agcaacacac aaacacaaaa aaacaatggg gattgttatg acacaacaag gggatgctgt
     4081 agccggggag ctggcgacag aaaaagtggg cattaaaggg tatctggctt tttttctcac
     4141 cataatcttt ttttccggtg ttttttccgg aacagacagc tggtggcgcg tttttgattt
     4201 ttccgttctg aacggatcgt ttgggcaact gcctggtgcg aacggagcca ccacctcatt
     4261 tcgcggggcg ggtggcgcgg gggctaaaga tggctttctc tttgcgctgg agctggcacc
     4321 gtcagtcatt ctctctctgg ggattatttc aattaccgat ggtcttggtg gattacgcgc
     4381 cgcgcagcag ttaatgacac ctgtgctaaa accgttgttg ggcattccag ggatctgctc
     4441 gctggcgctg atcgctaacc tgcaaaacac ggatgccgcc gccggtatga ccaaagagct
     4501 ggcgcaggaa ggggaaatta ccgaacgcga taaagtgatt ttcgccgcat atcaaaccag
     4561 cggtagcgcc atcaccacca actacttctc ttccggcgtc gcggtgtttg cctttctggg
     4621 cacgtccgta atcgtacctt tagccgtcat cctggtgttt aaatttgtcg gagccaacat
     4681 tttgcgcgtc tggcttaact tcgaagaacg tcgcaaccca acgcaaggag cgcaagcatg
     4741 acaactcagg tccgcaaaaa tgtcatggat atgtttatcg atggtgcgcg ccgtggcttt
     4801 actatcgcca cgaccaacct gctgccaaac gtggtgatgg cgtttgtcat cattcaggcg
     4861 ctgaaaatta ccggcctgct cgactgggtg gggcatatct gtgaacccgt aatggcgctg
     4921 tggggattac ccggtgaagc ggcaaccgtc cttttagccg cattaatgag tatgggcggc
     4981 gcagtcggtg ttgccgccag tctggcgacc gccggcgcat taaccggtca cgatgtcacc
     5041 gttttactgc ccgcgatgta tttaatgggc aacccggtgc aaaatgtcgg tcgctgcctg
     5101 gggacagccg aagtgaatgc caaatattac ccgcatatca ttacggtctg cgtgattaac
     5161 gcattgctgt cgatctgggt tatgcagctt attgtttaac aaggagttac catgattgat
     5221 tataccgcag ccggttttac cctgctgcag ggagcgcatt tgtatgcgcc ggaagatcgg
     5281 ggaatttgcg atgtcctcgt cgctaacggc aaaattatcg ccgttgccag caatatccct
     5341 tctgacattg taccggactg cacggttgtc gatctcagcg ggcagatcct ctgtcctggt
     5401 tttattgatc aacacgtcca tttgattggt ggtggcggcg aagcaggtcc cacgacgcgc
     5461 acgccagaag tggcgctaag tcgcctgacg gaagcgggcg tcacgtcagt ggttggtctg
     5521 ctgggcaccg actctatctc tcgccacccg gaatccctgc tcgccaagac ccgtgcgctc
     5581 aatgaagaag gcatcagcgc ctggatgctg accggcgctt atcatgtccc ttcccgcacc
     5641 attacaggtt ccgtggaaaa agacgtggcg attatcgatc gtgtgattgg cgtgaaatgc
     5701 gccatctctg atcaccgttc tgccgcaccg gacgtttatc acctggctaa tatggcggcg
     5761 gaatcccgcg ttggcggttt gctcggcggt aaacctggcg tcaccgtgtt ccacatgggc
     5821 gacagcaaaa aggcgttaca gcctgtctat gacctgctgg aaaactgcga tgtgccgatc
     5881 agcaagttgc tgccgaccca cgttaaccgc aacgttccgt tgtttgagca ggcgctggag
     5941 ttcgcgcgca aaggcggcac tatcgatatc accagcagca ttgacgaacc ggtcgcccct
     6001 gccgaaggta ttgcccgcgc cgttcaggcg ggtattccgc tggcacgcgt caccctcagc
     6061 tccgacggca acggtagcca gccgttcttc gatgacgaag ggaatttaac ccatatcggt
     6121 gttgccggtt ttgaaacgtt gctggaaacc gtgcaggtgc tggtcaaaga ctatgatttc
     6181 agtatcagcg atgccctgcg cccgctcacc agtagcgtag ccggtttcct taacctgacc
     6241 gggaaaggcg aaattctgcc aggcaatgat gctgacttgc tggtcatgac gccagaactg
     6301 cgcattgagc aggtatacgc tcgcggcaaa ctgatggtca aagacggcaa agcctgcgtg
     6361 aaaggaacgt ttgaaacggc ttaaacggtt gtcagccgcc cgaatggacg tagaatgccc
     6421 gcaagggcgg ctgacagagt aaaacgtaat ggatgacagt ggtgcgattt tgcataatat
     6481 tgaaaccaaa tggctttatg attttctgac cctggaaaaa tgccgcaatt tttcccaggc
     6541 ggcagtcagt cgcaacgtct cgcaaccggc attcagccgc cgcatccgtg cgctggaaca
     6601 ggcgattggt gttgaattgt ttaaccgcca ggtaacgccg ctgcaactct cagaacaagg
     6661 gaaaatcttc cattcgcaga tccgccatct gttgcaacag ttagagagca atctggcaga
     6721 gctgcgtggc ggcagcgatt acgcgcaacg aaaaatcaag atagccgctg cacactctct
     6781 ttccctcggg ctgttaccgt ccattatcag ccagatgccg ccgctcttta cctgggcaat
     6841 tgaagctatt gatgtcgatg aagcggtcga taaactgcgt gaagggcaaa gtgactgtat
     6901 tttttccttt cacgacgaag atttgctgga agcgccgttt gaccacattc gcttatttga
     6961 atctcaattg ttccctgtct gcgccagtga cgaacacgga gaagcacttt ttaacctcgc
     7021 gcagccacac tttccgttac tgaattacag ccgcaactcc tacatggggc gattgattaa
     7081 tcgcaccctg acgcgccaca gtgagttaag tttcagcacc ttttttgtct cttcgatgag
     7141 cgagctttta aagcaggttg ccctcgacgg ctgtgggatt gcctggctgc cggagtacgc
     7201 catccaacaa gaaattcgca gcgggaagct cgttgtactg aatcgggacg aactggtgat
     7261 cccgattcag gcctatgcat accgaatgaa tacccgcatg aatccagttg ccgaacgctt
     7321 ctggcgtgaa ctgcgcgagc tggagattgt gcttagctga cattctccag catcgcacta
     7381 caccaaccgc cattatccca gaccagatca aacgttagcg gttcaccgtc gtggtacagc
     7441 attatctcaa ggctgatcgc cagagtgtgg cagtacgctt ctttaaaatt aacctgcaac
     7501 gccgcaatcc gtggctcatg aaataacagt gcagaacgca gctgacggca ataccagctc
     7561 tggagatgtt tgtcgctctg gttatgcact tgccactctg gcatgcctga aacaagtaat
     7621 gacggtaatg ccgaatgagg agaattaaaa agagtgagga tatcgctgca taccgattta
     7681 cgcagcaatg acgttttatt gcctgaaatt tcaggtaaaa cagcctgaag tgattggcgc
     7741 atcattttgc gcactcctga cgtttagcaa catatgcggc ttgccctgaa caccgggcac
     7801 cactgtattg atgctcattt tccatagcca ttcccttttg ttcattacat ccatgtcagt
     7861 ctgagacagg cctgaatcct gtcagcaaag tataaaacct tacttgccta tgaatatcta
     7921 ctgacttttc aggaaaaaag tcatgatttg ataaaaatca tcaaactcaa aaaaaaaccg
     7981 ctagtatatt ctgaatattc cgaatagtta tgtagatgaa aatacttgtc accgccgcta
     8041 cttaattccg atgggaaatt atttatttcc gcataatcag ccatgtatta tttgaaacaa
     8101 ttagtttgta aataatataa attgataaca tatcaaatac atcaaaaata ccattcacag
     8161 gcattattaa tatagacatc aatatagcaa catcaaattc caattagtaa taagatatgc
     8221 tttttaattg catgaaaata atttcaaatg agcgtttttt gatactttag agtgtttcct
     8281 accctttaat atcaccgtgt agagttgata tcactacaac agaaacatgc atgtcatttg
     8341 tttacttctc tttttactac agaaaaattt atctattttt ctaaacatca gtgaaatact
     8401 cctaaaaggt aactattatg tctatgccat taagcaatgc ccttcagagt caaattatta
     8461 cagataatca cttccttcat cacccaaaag tcgacaatga gttaacacgt aaatatgaac
     8521 gagctcgcct ggatacggaa aatatctatc ttctgcctct tgctcgtggc aataaccata
     8581 attacgatgg aaaaagcgtt gtcgaaatta gaaaacttga tattagtaaa gagtcctggc
     8641 catttaatta tgtgacagaa gcatgcagag aatccgatgg tattacaaca acaggacgta
     8701 tgctctatcg aaacttgaaa atcacctcag ctttagacga aatatatggt ggtatatgta
     8761 aaaaggcgca tgctgctaca gaattagcag aaggtttacg gctaaattta tttatgaaga
     8821 gccctttcga tcctgttgag gactatacag ttcatgaaat aacattaggc cctggttgca
     8881 atgttccagg ttacgccgga accacgatag gatatatttc aacccttcca gtttctcagg
     8941 caaaaagatg gaccaatgag cagccaagaa ttgatatcta tattgatcag ataataactg
     9001 tctcaggggt agcaaattct tccggattcg cccttgccgc attatttaat gctaatattg
     9061 gaatgggaaa tgacccaata attggtatag aagcctatcc cggtaccgcg gagatccatg
     9121 caaaaatggg atataacgtt atccccggtg atgaagatgc gccactcaaa cgcatgactc
     9181 tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa tggtgaatgg aattatattg
     9241 gtaaatagcc actgagaaga atgagtacta atattcaggt tatacagata tttaaacaat
     9301 accctgcaat ttacaaagta atactcattg cgacttctta atttaagaaa gcgcaaggaa
     9361 cgtttaccct tgcgcttatt ataataagtc aggcgtcgac tttatccagc ggccatgaat
     9421 caaacagata gccgtcaaaa taaatatcgt caacgttcga gaattccagc agacgttgct
     9481 taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct tttttgatta
     9541 acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg ctgtcatcca
     9601 gatggctgtg caactggatc cacattgggt tgttttcccg ccactgccag gactgacgga
     9661 acagctccac cagcatgctg ttatgcgttg cttcggcaat agcgagatgg aactgcatgt
     9721 caccgctttc gctgctgccc ggcgcactgg aagccagttc acgctcttcc agttgcaatg
     9781 cctgacgcat tttgacgata tcttcgcggg tagcctgcag ggcggcaaac tcggcgatgt
     9841 tactttccag taactgacgc gcctgtaaca gctcaaaagg acccgcatca ttgcagacgt
     9901 tggcatccgg actgtctgtg ttgtggctgc ctgagctatc aagaacatag ataccggcac
     9961 cccggcgcac ttccaccagc cctttgatct ccagcatgat cagcgcttca cgtaccaccg
    10021 tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt tcgccagggt
    10081 tgtacggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg taaggtcttt
    10141 gcgcagaggt ggcagatttc atggtgttgt gtccgttagg tgcggttaat caaatttttg
    10201 ccggatg
//
LOCUS       DAFATA010000134        10207 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFATA010000134 DAFATA010000000
VERSION     DAFATA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.135"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1239
                     /locus_tag="LGJ20_004240"
     CDS             <1..1239
                     /locus_tag="LGJ20_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBK2388239.1"
                     /translation="SVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPS
                     NTVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGE
                     PFIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQH
                     NGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMS
                     ATQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDF
                     SRRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQR
                     QVFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNN
                     EQRSAVRERLSERGARRQER"
     gene            complement(1310..2734)
                     /gene="lpdA"
                     /locus_tag="LGJ20_004241"
     CDS             complement(1310..2734)
                     /gene="lpdA"
                     /locus_tag="LGJ20_004241"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK2388240.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(3059..4951)
                     /gene="aceF"
                     /locus_tag="LGJ20_004242"
     CDS             complement(3059..4951)
                     /gene="aceF"
                     /locus_tag="LGJ20_004242"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBK2388241.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(4966..7629)
                     /gene="aceE"
                     /locus_tag="LGJ20_004243"
     CDS             complement(4966..7629)
                     /gene="aceE"
                     /locus_tag="LGJ20_004243"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBK2388242.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(7790..8554)
                     /gene="pdhR"
                     /locus_tag="LGJ20_004244"
     CDS             complement(7790..8554)
                     /gene="pdhR"
                     /locus_tag="LGJ20_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBK2388243.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            9095..>10207
                     /locus_tag="LGJ20_004245"
     CDS             9095..>10207
                     /locus_tag="LGJ20_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2388244.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALV"
BASE COUNT         2312 a         2779 c         2551 g         2565 t
ORIGIN      
        1 tcggtacagc gattgcggca actggcgcaa caaaccggct cgctgaagtc atcaaccgaa
       61 cagaaagtta ttaccacaac gaagaaagct gtaccggtaa cacagacagt cacggctccc
      121 gtcataccat ccaataccgt ttcaactgcc agccccgtca ttacagagcc tgcaacaacc
      181 gtcatttcca ttgagcccgc caatcctgat gtggtctata ttcccaacta caacccaacc
      241 gtggtttacg ggaactgggc caatactgcg tatccgccgg tttatctgcc accaccagcc
      301 ggagaaccgt ttattgacag ctttgtgcgc ggattcggct atagcatggg tgttgctacc
      361 acgtacgcac tattcagcag catcgactgg gacgacgacg atcatgacca tcatcatcat
      421 gacgatgatg attatcatca ccacgatggc ggtcatcgtg acggtaatgg ctggcaacac
      481 aacggcgaca acatcaatat cgacgtcaac aatttcaacc gtatcaccgg tgagcatctt
      541 actgataaga atatggcatg gcggcacaat ccaaactacc gtaatggtgt gccctatcat
      601 gatcaggata tggcaaagcg gtttcatcaa accgatgtca acggcggaat gagcgccacg
      661 cagctacctg ctccaacacg cgacagccag cgtcaggcgg cagcaagtca gtttcagcaa
      721 cgaacacacg ccgcccccgt cattacacga gatacccaac gtcaggcagc ggcacagcgg
      781 tttaatgaag ctgaaaacta tgggagctat gacgacttcc gcgacttcag ccgtcgccaa
      841 ccactgaccc agcaacaaaa ggacgccgct cgtcagcgtt atcagtcggc ctcgcctgag
      901 cagcgccagg cagtccgcga gaaaatgcag actaacccac agatccagca gcgaagagac
      961 gcagcgcgtg agcgtattca gtcagcctcg cctgagcagc gccaggtgtt taaggaaaaa
     1021 gtacagcagc gcccactgaa ccaacagcaa cgtgataacg cccgccagcg tgttcaatca
     1081 gcatcacctg aacaacgtca ggtttttcgg gaaaaagttc aggagagccg cccacaacgt
     1141 ctaaacgaca gtaaccatac tgtcaggctg aataacgagc aacggtcagc agtacgcgaa
     1201 cgtctctctg agcgcggagc aaggcgacag gaaaggtaaa ttgcagacgt aaaaaaagcg
     1261 gcgtggttag ccgctttttt aattgccgga tgttccggca aacgaacact tacttcttct
     1321 tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca
     1381 cagactcgtg cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac
     1441 ccatttcgat tgccaggccg atttcaccca gcagctcgcc gccgttggta ccgacaatcg
     1501 caccaccgat cacacggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc
     1561 agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcatagctga
     1621 tgcctttctc tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat
     1681 aggcgatgga cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag
     1741 cggcaacgtg accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc
     1801 cgatagcaaa gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac
     1861 ggtcgtccac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac
     1921 caatcgctac cagcacggcg tcgtaacgct gcggttcagc gggtgctttt ttgccttcca
     1981 tcgtcacata aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca
     2041 ggttgaattt cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg
     2101 ggataacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtatacgg
     2161 tgcccatttc cagaccgatg ataccgccac ccattaccag caggcgttct ggtacttctt
     2221 tcagttccag cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca
     2281 gttggatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc acggttttac
     2341 cgttctcacc ttcaacttcc agggtgttag ccccggtaaa tttacccaga ccgttgacca
     2401 ctttgacttt gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt
     2461 tctctttcca ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag acgataccgt
     2521 gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag
     2581 ggatacagcc gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg
     2641 tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa
     2701 gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg
     2761 cggtcatacc cgtcgtcttt caggccgcag cggcgttagc ttcacgccct caccccagtc
     2821 acttacttat gtaagctcct ggggattctg gcgcttgcta ccttgctgca cccagaaatc
     2881 catagggtat gtgaagtaat accctaacca ccaccgggtc gttctatccg tcggtatttt
     2941 acaaaattgt taacaatttt taaacaacaa acggcaaccg atttgtctat tcgctaataa
     3001 cctcaataca ttcatgagat taccagaaaa aagccggccg ttgggccggc tcttttactt
     3061 acatcaccag acggcgaatg tcagacagcg tgttgttaat gatggtaatg aaacgggcac
     3121 catcagcacc gtcgatcacg cggtggtcga aggagagaga aatcggcagc atcagacgcg
     3181 gcacgaactc tttaccattc cacaccggct ccatcgcgga cttggaaacg ccgaggatag
     3241 ccacttccgg cgcgttcaca atcggcgcga agtgggtagt acccaggccg ccgatgctgg
     3301 agatggtgaa gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct
     3361 tagaaatagt catcagctcg cgagacagct cgatgatgcc tttcttgttg acgtctttga
     3421 ataccggaac aaccagaccg ttcggggtat ccaccgccac accgatgttg atgtatttct
     3481 tcagggtcag acgctgaccg tcttccgaca acgaactgtt gaagcgaggc atctgctcaa
     3541 gagctgcagc aacggctttc atgatgaaga caaccggggt gatcttcaca tccagcttac
     3601 gtttcgccgc ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg atatcggttt
     3661 tgtcgaagtg agtaacatgc gggatcatca cccagttacg gctcaggttc gcaccagaaa
     3721 ttttctggat acggcccagt tccacttctt cgatttcacc aaacttgctg aagtccacct
     3781 tcggccacgg cagcatgcct gggataccgc cgccagtcgc cgccggagct gcttctgcac
     3841 gtttgatagc ttctttcacg taagcctgaa cgtcttcgcg caggatacga cctttacggc
     3901 cagtgccctt cactttcgcc aggttaacgc caaactcgcg tgccagacgg cggatcagcg
     3961 gagtcgcgtg aacgtaagcg tcgttttctg caaattcaga tttgccttcc gctttcgcag
     4021 ccggtgctgc tgccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt
     4081 tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgagccag
     4141 ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg
     4201 gaacttccat agaagctttg tcgccttcta cggtaatcag tgactgttca gcggcaactt
     4261 tgtcgcccac tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa
     4321 cgttaacttc tttcacgcca gccgctggtg caggggccgc tgccggagcc gcttcctgtt
     4381 tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc
     4441 cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtgcca gcaaacggag
     4501 ccggaacttc catagacgct ttatcgcctt ctacggtgat cagcgactgt tcagcttcaa
     4561 ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg
     4621 gaacgttaac gtcttttgcc gctgcagccg ctggtgctgc tgccggagct gtttctttct
     4681 tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa
     4741 tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg
     4801 cctgcggaga cggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt
     4861 cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc
     4921 cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg
     4981 gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc
     5041 gatttcgcca cgtttagcca gttcgcccag cgccgctaca accacgtagg aagcatcaac
     5101 ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag
     5161 tacgcggtag tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata
     5221 gtcggtagat gccactgccg gagcgtcgtt catcacctga gcgatatacg gaacgcgcgg
     5281 agtttccagc gggtgcagca tgttccagcg ttcacaatcc tgaccatctc gcgccagttc
     5341 ggtgaaggag gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc
     5401 tgccgcttca cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt
     5461 gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc
     5521 cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc
     5581 ttgtttttca ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc
     5641 gtaagccggg tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc
     5701 atcttcgtgc tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag
     5761 gaagccacgc gcttgctggt cgccagccgc ccagcacaga tcgccgatac gctggaagcc
     5821 gaacatcgag taatagatgt agaacggaat catcggcaga ttgttggtgc tgtaagaggt
     5881 cgccgctgcc agccaggaac aacctgcgcc caactcgttg atcccttcct gcagaatctg
     5941 acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg
     6001 accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc
     6061 ttcgtcggcg atgatcggta ccagacgatc tttgatcgac ttgttcttca gcattacgtt
     6121 cagggcacga acgaaagcga tagtggtaga gatctctttg ctctgctctt ccagcagcgc
     6181 gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg
     6241 cagataaccg tgcagtttct gacgctgtgc gtgcagatag gtatgctctt cagaaccttc
     6301 cgggaaggtg atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg
     6361 gtcgcggatg tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt
     6421 accttcagcc gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc
     6481 tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc
     6541 accacggttc agtgcccaga tctgttcgtc agtccagtct gcaaccagtg ctgcggtttc
     6601 cggatattta ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg
     6661 gtagtcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg
     6721 cagcagttca tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc
     6781 gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg
     6841 ctgcaggtta cagttgataa cgaagaccag gttatccagt ttttcacggg tagcgatggt
     6901 gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt
     6961 ctgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta
     7021 aatagcacca atcggcccca gacccataga tacggtcggg aactgccaga attccggcat
     7081 cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc
     7141 cagctgctcc tgagtcagac gaccttccag gaaagcacga gcgtacacgc ccggggagat
     7201 gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg
     7261 gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag
     7321 ttcgagatct tttttcgacg cacgcagcac cgtcatgatg gcgttccagc gaatagctga
     7381 acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat
     7441 ggtgttgatg tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc
     7501 ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac
     7561 cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg
     7621 ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca
     7681 ttgaactctc gccggaaagc tcaataagac aggttctacg tttagttgcc gcgcttttat
     7741 atgcgcttga tttacaacat cttctggata atttttacca gaaaaatcac taattctttc
     7801 gttgctccag acgacgcaga gaacgctcac ggcggctctc ttcacgactt ctgtcgagca
     7861 aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac
     7921 cggccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc
     7981 gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac
     8041 accttagcag atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact
     8101 ggagtacggc gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt
     8161 ggtggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag
     8221 cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgac tcaggatggt
     8281 cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag
     8341 tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac
     8401 gcaaggaggg acgggagacg tcaaactgtt ttgccagttc gcgttccggt gggagttttt
     8461 cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg
     8521 agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg
     8581 aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt
     8641 atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata
     8701 tataacgttt caaagttgta actatgcaca aatgtagact ttacgtagga aaggagtttt
     8761 ttaacgatta ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttgtgat
     8821 ttttaattca attaaaacga atttaaattc attctacata ttgagagggg ttgaggctga
     8881 gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat
     8941 tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtagca attcaaactt
     9001 ctttgatgta aacaaattaa tacaacaaac ggaattgcaa acttacacac gcatcactgc
     9061 gtagatcaaa aaaacaacca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga
     9121 gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat
     9181 agggaccggg ttattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat
     9241 cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat
     9301 ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag
     9361 ttttgccggt ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc
     9421 tgagctgact gccgtgggta aatacattca gttctggtat ccggaaatcc ccacctgggt
     9481 ttctgccgcc gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt
     9541 tggcgagatg gagttctggt ttgccattat caaagttatc gcggtggtag cgatgatcat
     9601 cttcggcggc tggctgctat tcagtggcaa cggcggcccg caggcgaccg ttagcaacct
     9661 gtgggatcag ggtggtttcc tgccgcacgg cttcaccggg ctggtgatga tgatggcgat
     9721 tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa
     9781 cccggagcaa agtataccga aagcaactaa ccaggttatc taccgcatcc tgattttcta
     9841 tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac
     9901 cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa
     9961 catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg
    10021 tatgctgttt ggtctggcac aacagggtaa tgcgccaaaa gcgctggcgt ctgtcgataa
    10081 acgtggtgta ccagtaaata ccattctggt gtctgcactg gtaacggcgt tgtgcgtact
    10141 gattaactac cttgccccag agtccgcttt cggactgtta atggcgctgg tggtatctgc
    10201 actggta
//
LOCUS       DAFATA010000135        10178 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFATA010000135 DAFATA010000000
VERSION     DAFATA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.136"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..302)
                     /gene="nikC"
                     /locus_tag="LGJ20_004246"
     CDS             complement(<1..302)
                     /gene="nikC"
                     /locus_tag="LGJ20_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="with NikABDE is involved in nickel transport into
                     the cell; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBK2388245.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGG"
     gene            complement(299..1243)
                     /gene="nikB"
                     /locus_tag="LGJ20_004247"
     CDS             complement(299..1243)
                     /gene="nikB"
                     /locus_tag="LGJ20_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBK2388246.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRNRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(1243..2817)
                     /gene="nikA"
                     /locus_tag="LGJ20_004248"
     CDS             complement(1243..2817)
                     /gene="nikA"
                     /locus_tag="LGJ20_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBK2388247.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(2928..3515)
                     /gene="acpT"
                     /locus_tag="LGJ20_004249"
     CDS             complement(2928..3515)
                     /gene="acpT"
                     /locus_tag="LGJ20_004249"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBK2388248.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALFSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(3570..4619)
                     /locus_tag="LGJ20_004250"
     CDS             complement(3570..4619)
                     /locus_tag="LGJ20_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK2388249.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            4751..5968
                     /locus_tag="LGJ20_004251"
     CDS             4751..5968
                     /locus_tag="LGJ20_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388250.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCQGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(5972..6529)
                     /gene="dcrB"
                     /locus_tag="LGJ20_004252"
     CDS             complement(5972..6529)
                     /gene="dcrB"
                     /locus_tag="LGJ20_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBK2388251.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(6602..7267)
                     /locus_tag="LGJ20_004253"
     CDS             complement(6602..7267)
                     /locus_tag="LGJ20_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBK2388252.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            7488..7733
                     /gene="tusA"
                     /locus_tag="LGJ20_004254"
     CDS             7488..7733
                     /gene="tusA"
                     /locus_tag="LGJ20_004254"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBK2388253.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(7835..10033)
                     /gene="zntA"
                     /locus_tag="LGJ20_004255"
     CDS             complement(7835..10033)
                     /gene="zntA"
                     /locus_tag="LGJ20_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBK2388254.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTAVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAAEEPQASRLKENLSLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(10107..>10178)
                     /locus_tag="LGJ20_004256"
     CDS             complement(10107..>10178)
                     /locus_tag="LGJ20_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBK2388255.1"
                     /translation="ADNAIAAACYFAGHFLIVRSLYL"
BASE COUNT         2316 a         2772 c         2788 g         2302 t
ORIGIN      
        1 ccgccaatca gtccggcgct gccgccaatc accagcccta atgtcagcac cagcagcagg
       61 caggccatta ccgaaccgag cgacacgcgg gtcgccgcca tcagccgcga gaaaatatca
      121 cgccctaagt gatcggtgcc cagccagtgc tgcgcatccg gcgaaagcag gcgcgacggc
      181 agatcaatag cctgagggtc atacggcagc caccactggc tggtaagcgc aatcagcgcc
      241 agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag gaaaaagttc
      301 acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg agcaaatcga
      361 caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg
      421 gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga
      481 tgttttcgat aatcatcgtc ccgccgatca gttcgccgat gtgcatcccc acggcggtga
      541 tcatcggcag cgaggcattg cgcaggatgt ggcgacgttc ggtctgtttg tcgttcaggc
      601 cgcgcagacg cgcccaggtg acgtgacgct gaccggcgac gtccagcata ctggcgcgca
      661 gtaaacgcgc gttaatcgcc agcgacataa aggcaatgga aaccgcaggc aaaatgatgt
      721 gctgccagcc gccgtaaccc atcgcgggta gccattgcag atacaccgaa aacgccatca
      781 ccagtaaaaa cgccagccag aagttaggca tcgacacgcc aagaaacgcg atgaaacgta
      841 cggcgaaatc cggcagacgg ttgcggtggc gcgccgccca gataccgagc ggtacggaag
      901 tgagcagaat taataccagc gccgcacctg caagttccag cgtggcgggc aggaagttca
      961 gcatatcgtc cagtaccggg cgttggctgg cgaatgagat accaaagtca agatgcagcg
     1021 ccttccacaa ccaggtgccg tactggacgt acagcggctg atccagtccc agcatggtgc
     1081 gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga cagacgcaaa taatcgagcg
     1141 ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg gcgagcacca
     1201 tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt tcaccggttt
     1261 aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatgttac ccagctccgg
     1321 ttttgatacc accatcatcg agatgtagct gataggcaga taaaccgcct cgtcatgcag
     1381 acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac
     1441 ttcgccgatc tctttatcaa tcagcggctt gtcggctaat ccttgctgtg cctggaagtc
     1501 agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca
     1561 ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc
     1621 ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct gaatgatttc
     1681 cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg
     1741 accatttttc tcgcggatgt ctttgcccgc aggcagcgtc caaccggctt tttccagcaa
     1801 ctctttcgct ttctgcggat cgtactggcg cggtttcagg ccgagattag cgtaaggcac
     1861 agaaggggca aacagggtgt cggcgacctg ctgggtgcca tataacgcgt tatcaatcag
     1921 cgattttttg tttaccgcgt aattaagagc ttcacgtact gccagctcgt tggtgggggc
     1981 tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gggtgtggta
     2041 agccggattc tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta
     2101 cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt ccgggatgac
     2161 gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt tacggacgaa
     2221 gacatcgtac tgattcagtt tcgattcctg caaaatccac ggtccggtgc caatcggcgc
     2281 tttaattccg ttcatggttt catggttttt aaactgcgag ggagcgataa aacggaaagg
     2341 acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg taatttgcag
     2401 ctctgtttta ctgagtgctt taacatcaac aatctggttt gccagctcca gccaggcgtg
     2461 acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctcgg catcgaacgg
     2521 ttcaccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt taccatcttc
     2581 tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac
     2641 caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa ggtgcgggtt
     2701 tagtggcccg acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac
     2761 gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg agagcatggt
     2821 aaccccaatg gattaaaata ggtggcggaa ataagtatga cgattttaag tattcgtcat
     2881 actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa
     2941 tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg tgggggtgca gatcgccagg
     3001 cttaaatttt caagctgaca atggctgact gacagcgagg agtgataggt gctgtctacg
     3061 ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag
     3121 atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatctc agcgtgttcc
     3181 ccgaggctga atacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg
     3241 atatcgcagc cgacttcgcc ttcatcactc aacagcagag cgatatcgtc accgctatgg
     3301 cttaagttga accatagcgg cgtttccggc gcgaatgcag gtttgccttg ttcgccatag
     3361 atgatctccg gtagcgggga aagcgtgtgc gaaaacaatg cacgccccgc cagccagcgt
     3421 tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg gtggcagtgg agctgcgctt
     3481 aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt taatggaaaa
     3541 tgaaagtgcg tatcgtatca cttgtcgcct catcccggta accgactttt cggtctgccc
     3601 ggccccagta aaatcgccag tttgctgcca cctttcgtcg tttccatcca gattttacac
     3661 acgctggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca tccccagatt
     3721 aacaatgaaa gaaataccac catggtggac atccccaggc gatggcccat catccgtggc
     3781 tctaaaatat tgccgatgac catatggaca actaaaaaca atgcgccgac cagaatacat
     3841 tcgtaaatac cattaaacag cagcacctga atcattggcg gtacggcgga aattaccgcg
     3901 ccgatattgg gcacgtagtt aagcaaaaac gccagtactg cccacatcag cgcaaactgt
     3961 acccccatca gctccagccc cagccagacg atgacacctg tccataaact gagtagcgtc
     4021 ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt gtaatcccgc gatgtgaatc
     4081 tgtggattat tcagcgcaaa acgcattttg taagggacgt ggcgcacttc aaacagcata
     4141 aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag cccggtcatt
     4201 agcgctgtgg tgaaggtaac cactttttcc gagtccatcc gctgcagcat tcgctccggc
     4261 gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaagttt gcgcgtcagc
     4321 tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc cagtacgccg
     4381 accagcgcgg ttagtgtgat cagcatcacc accaccacaa tcgtaatggc aacggggcgt
     4441 tttactcccc gtcgaataaa ccaggtaacg agcgggttga ggacgatggc aaaaaacagc
     4501 gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc
     4561 agcgaggcca gcttgagcag aatgtgcatg cccgttttat cgggttgagg ggtttccatt
     4621 ggggcttcct tgtgactttt tgtattaagt gtagcgggag tcacgccagc aatattctga
     4681 ttcttactgc tgattttcac gtcagcacat ggtaaaaatg aaacactgtt gtaaaaatgt
     4741 ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt
     4801 gcgcattgtc tccattgtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct
     4861 cgctgtatta ccgggctatg tccatgatgt gatgggcttt agcgcttttt gggcaggatt
     4921 ggttatcagc ctgcaatact tcgccacctt gctgagccgc cctcatgccg gacgttacgc
     4981 cgatttgctg ggacccaaaa agattgtcgt cttcggttta tgcggctgct ttttgagcgg
     5041 tctgggatat ctgacggcag gattaaccgc cagtctgccc gtcatcagcc tgttattact
     5101 ttgccaggga cgcgtgatcc ttgggattgg gcaaagtttt gccggaacgg gatcgaccct
     5161 gtggggcgtt ggcgtggttg gctcgctgca tatcgggcgg gtgatttcgt ggaacggcat
     5221 tgtcacttat ggggcgatgg cgatgggtgc gccgttaggc gtcgtgtttt atcactgggg
     5281 cggcttgcag gcgttagcgt taatcattat gggcgtggcg ctggtggcca ttttgttggc
     5341 gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gcgcggtgct
     5401 tgggagcgtc tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgtcat
     5461 cgccaccttt atcacgctgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct
     5521 gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg
     5581 tatcggtggc ttaaacgtag cgatgatttg ctttagcgtt gagataatcg gcctgctact
     5641 ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggccgg
     5701 gttttcgctg gtgttcccgg cattgggtgt agtggcggta aaagcggttc cgcagcaaaa
     5761 tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgactgg
     5821 accactggct gggctggtga tgagctgggc gggcgtaccg gtgatttatc tggcggcggc
     5881 gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ctccggaaca
     5941 cgtccctgag gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt
     6001 tttctgcggt ggtctgcgct ttttgctgat cgtcagcggg cagcgtaatt tgcatggtca
     6061 gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg
     6121 agataatact gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta
     6181 ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccagcaccg
     6241 ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttctgc ccggtggcgt
     6301 cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat
     6361 ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtagcgt
     6421 tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa
     6481 gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc
     6541 tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc
     6601 tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc cagtcttttc aacagcatat
     6661 tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt
     6721 aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga
     6781 aggcaatgaa gaaaaaggcc agcgtgtcac tgacgttacc gaacagtgtg gacgctgtcg
     6841 gtgccagcca ccagcggcga ctttgacgca ggcggttaaa aacgtgcacg tcgaggattt
     6901 gccccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt
     6961 gggcgagtgc gccgaatccc tgccatgaac ccatatagaa cagcgacgag atgacatagg
     7021 agattaataa cgcagggatc attaccgcga agataatgcg tcgggccagc ggtgcgccaa
     7081 aaatacgcac ggtcaggtcg gtcgcgagaa agataaacgg aaaactaaac gcgccccagg
     7141 tggtatgaaa accaaaaatg gagaccggaa gctggaccag atagttactg gaggtgatca
     7201 ccagcagatg aaataacgat aaccagaaca acgccttata gcgttgagtt tgcgagaaaa
     7261 cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata cgtacgacac
     7321 gtctgttatc ttaacataat aacgtgctta cccttttttc gagccgccgc atgatactgc
     7381 tttacgttga caatgcaatg gttaattttc acgcaatcgt taacctggtt tgcttacgga
     7441 ctcacagggc gtaaactagc gccgtttttt taagtgatga gaagaaaatg accgatctct
     7501 tttccagccc tgaccacaca ctcgacgcgc ttggcctgcg ctgcccggaa ccggtgatga
     7561 tggtgcgcaa aaccgtgcgc aatatgcagc ctggcgaaac tttactgatt atcgccgacg
     7621 atccggcgac gacccgcgat attcctgggt tttgtacctt tatggaacac gaactggttg
     7681 cgaaagagac ggatggactg ccttatcgtt atttgattcg taaaggcggt tgatggggaa
     7741 agattggctt cgatgccgcc ttttcccctc accctaaccc tctccccaga ggagcgaggg
     7801 gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct cctgcgcaac aatcttaacg
     7861 cattcgctgt taccagcacg gtcgcaccgg tatctgccag caccgccagc catagtccgg
     7921 tcatccctag cagcgtggtg acgaggaaga tccctttcag ccccagcgca atagtgatgt
     7981 tctggcggat attggcgtga gtggcgcgtg ccagttcaat catttgcacc aggccgcgca
     8041 ggtggttatg ggttaatgct gcgtcggcgg tttccagcgc cacgtctgtg ccactgccca
     8101 ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc gttaatacca tcaccgacca
     8161 tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt gactttatct tccggcaata
     8221 ggcccgcttt aaactccagc cccagctccc cggcaattgc cgccgctgcg cgtggattat
     8281 cgccggtgag gatcactcct ttaaccccca gcgcgttcag ttcactgatg gcagttgctg
     8341 cgtcggcgcg cagggtatcc tgcaatgcaa tgatacccag cacgtcatca ttacgtacta
     8401 ccagcacaac cgtttgcccg gcgctttcca gttcattaat caaaccagca aatgcatcag
     8461 cgggatgttt cccggcagcg caaatcaata cgcgctcacc gttaacctgc gcttcaatgc
     8521 cagacccgac cagcgcccgc tgtgattcgg cggtgggaat ggcgagttca gcaacctgtg
     8581 cttcgcgtac gatggcctgc gccagtggat gcgtcgcgcc ttgctcgacc gccgccgcca
     8641 gtgtcagcag ttcagattca ctaatacccg ttgccggatg aatcgcggta acgcgcggtt
     8701 taccgacggt cagcgtaccg gttttatcaa acgccacctg agtaacacga cccagctgtt
     8761 ccagcgccgc tccgccttta atcaacgccc cacgacgtgc cgctgctgcc agcccggagg
     8821 taatcgccgc gggcgtggag ataactaacg cacacgggca gccaatcagc agcagcgtca
     8881 ggcctttata aatccactct tgccagctgg cggcaaacag cagcggcggc accagcgtta
     8941 ccagcagggc gacggccata attgctggcg tataaatgcg gctgaaacgg tcgataaacc
     9001 gctcaatggg ggcgcggcgc tcttcggctt cttcgatcag tttcagaatc cggtcaatgg
     9061 cgctggcacc cggttctgac agcacttcca gcgttaccag acggtctacg ctggtggctc
     9121 cagcagggac tttatcgccc gtcgcgcgct ccaccggaat ggattctccg gtcagggcgc
     9181 tttcatcaaa actggcaaac ggtgagagca atttaccgtc ggcaggcaaa cgcccacctg
     9241 cggcgacttc aatcacatcg ccagggcgca ggctgttaat cgccacctct tcccgctcac
     9301 cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat taacgcgctg accccttgac
     9361 gtgcgcggct ggcggcccag ccttccagtc gttcgccaat caaaaacagc agcaacacca
     9421 tcgcagcttc agccgttgcg ccaataaaca gcgcaccaat agcggctacg ctcattaacg
     9481 tttcaatggc gaagtagctg ccggatttga tcaaccgtaa tgcctgacga gcaatcgggt
     9541 acagcccaac cagcgtggtc gcgataaacg ccagttgccc gaacggatga ttgaactgct
     9601 ccagacccca gctgattgcc atcatcacga ttagcgtaat cagcgacaga ttctctttca
     9661 ggcgtgatgc ttgcggttct tcggcggcct gttcatcgcg cagggaatag cctgcttttt
     9721 gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc atcgaccacc agtttttcgg
     9781 tggcgaacaa cacctgcacc tgattcacgc ctgcaagctg gcgcacagca ttttctacct
     9841 tgcgcgcaca ggcggcgcag tccatgccgc tgactttcca gctatagcgg gtgccggaga
     9901 cgttttcaga gagcgttggc gagctggaac atgcgccgtc gcagcaacag tcgttggcgt
     9961 tctgtaccgc agttagcggt ttgaacgcag caaattgagg ggctttcttg ccgtgattgt
    10021 caggagtcga catggcatcc tccggttaag ttttttctca ttaaccgaag gatacactct
    10081 ggagtcgact ccagagtcaa gttttatcag agatacagcg agcggacgat gaggaagtga
    10141 ccggcaaagt agcaggccgc agcaatcgcg ttatccgc
//
LOCUS       DAFATA010000136        10119 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFATA010000136 DAFATA010000000
VERSION     DAFATA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.137"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..171
                     /locus_tag="LGJ20_004257"
     CDS             <1..171
                     /locus_tag="LGJ20_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HBK2388256.1"
                     /translation="GVGGTYDVFTGHVKRAPKIWQTLGLEWLYRLLSQPSRIKRQLRL
                     LRYLRWHYTGNL"
     gene            362..1747
                     /locus_tag="LGJ20_004258"
     CDS             362..1747
                     /locus_tag="LGJ20_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2388257.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            1850..1926
                     /locus_tag="LGJ20_004259"
     tRNA            1850..1926
                     /locus_tag="LGJ20_004259"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1884..1886,aa:Arg,seq:ccg)
     gene            1985..2060
                     /locus_tag="LGJ20_004260"
     tRNA            1985..2060
                     /locus_tag="LGJ20_004260"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2018..2020,aa:His,seq:gtg)
     gene            2081..2167
                     /locus_tag="LGJ20_004261"
     tRNA            2081..2167
                     /locus_tag="LGJ20_004261"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Leu,seq:cag)
     gene            2210..2286
                     /locus_tag="LGJ20_004262"
     tRNA            2210..2286
                     /locus_tag="LGJ20_004262"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2244..2246,aa:Pro,seq:tgg)
     gene            2433..3668
                     /locus_tag="LGJ20_004263"
     CDS             2433..3668
                     /locus_tag="LGJ20_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBK2388258.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3752..3913)
                     /locus_tag="LGJ20_004264"
     CDS             complement(3752..3913)
                     /locus_tag="LGJ20_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388259.1"
                     /translation="MEFSFSPKLFVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(4592..5788)
                     /gene="hemY"
                     /locus_tag="LGJ20_004265"
     CDS             complement(4592..5788)
                     /gene="hemY"
                     /locus_tag="LGJ20_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBK2388260.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(5791..7026)
                     /gene="hemX"
                     /locus_tag="LGJ20_004266"
     CDS             complement(5791..7026)
                     /gene="hemX"
                     /locus_tag="LGJ20_004266"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBK2388261.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQGDTPAAAPQGE"
     gene            complement(7048..7788)
                     /gene="hemD"
                     /locus_tag="LGJ20_004267"
     CDS             complement(7048..7788)
                     /gene="hemD"
                     /locus_tag="LGJ20_004267"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBK2388262.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(7785..8726)
                     /gene="hemC"
                     /locus_tag="LGJ20_004268"
     CDS             complement(7785..8726)
                     /gene="hemC"
                     /locus_tag="LGJ20_004268"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBK2388263.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            9113..>10119
                     /locus_tag="LGJ20_004269"
     CDS             9113..>10119
                     /locus_tag="LGJ20_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBK2388264.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     E"
BASE COUNT         2265 a         2557 c         2683 g         2614 t
ORIGIN      
        1 ggcgttggcg ggacttacga tgttttcacc ggtcacgtaa aacgcgcacc gaaaatctgg
       61 caaacgctgg gactggagtg gctctaccgc ctgctttcgc agccgagccg cattaagcgt
      121 cagcttcgtt tgctgcgtta tttacgctgg cactacaccg gcaacctctg attttcctct
      181 ctttgtaaag cgacggagtg gtcacttcgt cgcctgcctg cttttttatt acacaaagca
      241 ttcaaatttt taatgcttta tttgccattt ctcctgaatt acgaaaacat tcgcaacact
      301 cgatgtaccc ataacgataa ccggtaacac cggaaagcat gcaaacacaa cacgaggatt
      361 tatggcagat aacaaaccag agctacagcg tgggctggaa gctcgacata tcgaactcat
      421 cgccctgggg ggcaccattg gcgtcggcct gtttatgggg gccgccagta ccttgaaatg
      481 ggccgggcca tccgtattgt tggcctatat catcgccggg ctattcgtct ttttcatcat
      541 gcgttcaatg ggcgaaatgt tgttcctcga accggttacc ggttcgttcg ccgtttatgc
      601 gcatcgttat atgagcccgt tctttggtta tctcaccgcc tggtcttatt ggtttatgtg
      661 gatggcggtg gggatctcag aaatcaccgc cattggcgtt tatgtccagt tctggttccc
      721 ggagatggcg cagtggatac ccgcattgat cgcagtggcg ctggtggcgt tggcgaatct
      781 ggcggcggtg cggttgtacg gcgaaatcga gttctggttc gcgatgatca aagtcaccac
      841 gattatcgtg atgattgtca ttggcctggg cgtgattttc tttggctttg gcaatggcgg
      901 gcagtcgatt ggttttagca atctcacaga gcatggcggt ttctttgcgg gtggctggaa
      961 agggttcctg accgcgctgt gtattgtggt ggcgtcctac cagggcgtgg agctgattgg
     1021 cattactgcc ggtgaagcga agaatccgca ggtgacgctg cgcagtgccg taggcaaggt
     1081 gctgtggcgg atcctgattt tctacgtagg cgcgattttc gttatcgtca ccatcttccc
     1141 gtggaatgaa ataggcagca acggcagccc gttcgtactg acttttgcca aaatcggtat
     1201 taccgcagcg gcgggcatta tcaactttgt ggtgctgacg gctgcgctct ctggctgtaa
     1261 cagcggcatg tacagttgcg gacgtatgct ctacgcactg gcgaaaaacc gtcagttacc
     1321 ggcggcgatg gcgaaagttt cccgtcacgg cgtacccgtt gcgggtgtgg cagtatctat
     1381 tgctattctg ctaattggct catgcctgaa ctacatcatt cccaatccgc agcgtgtgtt
     1441 tgtctacgtc tacagtgcca gcgtgcttcc ggggatggtg ccatggtttg tgatattgat
     1501 aagccagctg cgttttcggc gtgcacataa agcggcgatt gccagccatc cgttccgctc
     1561 aatcctgttc ccgtgggcca actacgtaac aatggcattc ctgatttgcg ttttgatcgg
     1621 catgtacttt aatgaagata cgcgtatgtc gctgtttgtt ggcatcatct ttatgctggc
     1681 ggtgacggcg atttataaag tttttggcct taatcgccac gggaaagcgc ataaactgga
     1741 ggaataagca gcaaaacgca caaaccgtaa ccaaacgcgc aatttattta aaaagggact
     1801 agacagaggg gtgggaagtc cgtattatcc acccccgcaa cggcgctaag cgcccgtagc
     1861 tcagctggat agagcgctgc cctccggagg cagaggtctc aggttcgaat cctgtcgggc
     1921 gcgccattta gtcccggcgc ttgagctgcg gtggtagtaa taccgcgtaa caagatttgt
     1981 agtggtggct atagctcagt tggtagagcc ctggattgtg attccagttg tcgtgggttc
     2041 gaatcccatt agccacccca ttattagaag ttgtgacaat gcgaaggtgg cggaattggt
     2101 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct
     2161 cgcaccacga ctttaaagaa ttgaactaaa aattcaaaaa gcagtatttc ggcgagtagc
     2221 gcagcttggt agcgcaactg gtttgggacc agtgggtcgg aggttcgaat cctctctcgc
     2281 cgaccaattt tgaaccccgc ttcggcgggg ttttttgttt tctgtgcatt tcgtcaccct
     2341 cccttcgcaa taaacgcccg taataactca ttgccccacg gtatgatttc gcccttaacg
     2401 tattgaagga tgacttcagg caaggagtga ccatgctgca acaggttcca acgcgtgctt
     2461 ttcatgtgat ggcgaaaccg agtggttccg attgtaatct gaactgtgac tactgttttt
     2521 atctcgaaaa acaatccctt taccgcgaaa agccagtcac gcatatggac gatgacacgc
     2581 tggaagcgta tgtccgtcac tatatcgctg ccagcgaacc gcaaaacgaa gtggctttta
     2641 cctggcaggg cggcgaacca acgttactcg ggctggattt ttaccgccgt gccgtaaagt
     2701 tacaggcgaa atacggtgct ggcaggaaga taagtaacag cttccagact aacggcgtgc
     2761 tgctcgatga taaatggtgt gcatttctgg cagaaaatca ttttcttgtt gggttatcgc
     2821 tggacggtcc ggctgagatc cacaatcaat atcgcgtgac caaaggtggc agaccaacgc
     2881 ataagctggt gatgcgtgcc ctgacgctgc tgcaaaaaca tcatgtcgac tataacgtgc
     2941 tggtctgcgt caaccgcacc agcgcgcagc aaccgttgca ggtttatgat tttttgtgcg
     3001 atgcgggagt cgaattcatc cagtttattc cggtggtcga gcgcctggct gatgaaacag
     3061 ctgccagcga tggactgaaa ctacatgcgc ctggtgatat tcagggggaa ctgacggaat
     3121 ggtcggtgcg ccccgatgaa tttggtgaat ttctggtggc gatattcgac cactggatca
     3181 aacgcgacgt cggcaagatt ttcgtgatga atatcgaatg ggcgtttgcc aattttgtcg
     3241 gtgcgccggg tgcggtttgc catcatcagc caacctgtgg gcgctcggtg attgttgagc
     3301 acaacggcga cgtttacgcc tgcgatcact atgtttatcc gcaatatcgg ctggggaata
     3361 tgcatcagca aacaattgca gaaatgatcg attccccgca acagcaggtg tttggtgaag
     3421 ataaatttaa gcaattaccg gcgcagtgtc gcagttgtaa cgtgttaaaa gcgtgctggg
     3481 gaggctgccc gaaacaccgc ttcatgctcg atgccagcgg caaaccgggg ctgaattatt
     3541 tgtgtgccgg gtatcagcgt tatttccgcc atctaccgcc atatcttaaa gcaatggctg
     3601 atttgctggc gcacggtcgc ccggccagtg acattatgca ggcacatttg ctggtggtga
     3661 ataagtaaaa aagcggccat tttgtggccg cctgcggttg attgccggat gcggcgtaaa
     3721 cgccttatcc ggcctacatg atcgtgcaaa ttcaataaat tgcagcgttc tgtggatact
     3781 ggtttgggtg atcgtatccg gcaaagcctt tgcgtgcggt ggcagttgac ggggtatctg
     3841 ctgcgctggc catgagagga agagcggcgg cgacagcaac aacaaaaagt ttgggtgaaa
     3901 acgaaaattc catgcaaaat gctccggttt catgtcatca aaatgatgac gtaattaagc
     3961 attgataatt gagatccctc tccctgacag gatgattaca taaataatag tgacaaaaat
     4021 aaattattta tttatccaga aaatgaattg gaaaatcagg agagcgtttt caatcctacc
     4081 tctggcgcag ttgatatgta aggcaggttt attatatcgc gttgattatt gatgcttttt
     4141 ttagttttaa cggcaattaa tatatgtgtt attaattgaa tgaattttat cattcataat
     4201 aagtatgtgt aggatcaagc tcaggttaaa tattcactca ggaagttatt actcaggaag
     4261 caaagaggat tacagaatta tctcataaca agtgttaagg gatgttattt cccgattctc
     4321 tgtggcataa taaacgggta gatgctcatt ccatctctta tgttcgcctt agtgcctcat
     4381 aaactccgga atgacgcaga gccgtttacg gtgcttatcg tccactgaca gatgtcgctt
     4441 atgcctcatc agacaccatg gacacaacgt tgagtgaagc acccacttgt tgtcatacag
     4501 acctgtttta acgcctgctc cgtaataaga gcaggcgttt ttttatgcat cagaaaggtc
     4561 ccggaggtgc ttgcctccgg gtgagaagga actactgtgg tgggttgttc tgcaacgtta
     4621 acatcaaacc gtcgcgacgc atcgctgcag cttcttccgg cttgtgcagt ctgtccagcg
     4681 cgtcggcaag ccatgcgtaa tcgtaggcgt ccggacgttg tttcagcgcc gcgcggaagg
     4741 cgagcgatgc ttcctgccat tctccgtgct tcatcagtga ctggcccagt gtgctccaca
     4801 acagcgggcg atcgccgacg tttttgatct gctggcgcag cactttttcc agctgttccg
     4861 gattgttggt tttcagtcga ggaatcggca gcagtaggcg atcgtcgtac tggcgtttca
     4921 ggccatcgat gataatttgc tgggcagtat catgatcgtc acattcaata agatgttccg
     4981 ccattgccac ctgcaacgct acctgatgac gcgttttccg gctttggttt ttccaccagt
     5041 tacgcaaacc ttcactaccg ttatcggcac gcgcctgatc catcaggcca atccatgcct
     5101 gttgttccag cattgcacga tgttcttcat caccaacatg ggctttcgcc attgatggga
     5161 tgatatccag cagagaactc catgcacctg tgcggatata cgcctgttcc gccagacgta
     5221 atacttccgg atggcgtggc gtaacttcca gcagcttatc cacgccgtgg cgtgcagcat
     5281 ggttttcatt acgggccagt tgcagacgta cacgggtgat ttctaccgga atggtgtcat
     5341 tgccggccag ctctgctgcg cgttccagat gttggttggc gcgtgcttca tcaccacgtt
     5401 gttgcgcggc ttcggcagcc agtagatagt tcaccaccgg ttgttccgcg tgatcggcat
     5461 ttttcgccat cagcttttca acttgctgat aatcgccttc cgccagtttc agcagcgcct
     5521 gttcggtctg cttacgggca cggcgacgct tacgtccgac aaaccaccca cgggtgtgtg
     5581 cgcctgtgcg gaagatccgc cgcagtagcc actcaatggc aaacagcact accatcgcca
     5641 gaatcaaaat aatcgccagg cccgtgacgc tggtttcgat attgtagttg tcagtctgga
     5701 tcagcacgta accctgatga ccggcaatca tcgggccaac cactatcccc gcaatcagca
     5761 aaacaaagag taataacact tttagcatgg ttattctcct tgcggcgcgg ctgccggagt
     5821 atcaccttgc ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg
     5881 tgcaggtgca ggtgcaggtt tagcttccgt tgtccccgct gccggttgtg ccagcaggtt
     5941 acgcacgcgg gtctgcatca gtttttccag catcgcctgg ctttgcaggg tttccggaag
     6001 atccatcgag atattttgct ggcttaactg gtccacctcg tcgaggaacg ctttggtggt
     6061 ggcatcatca gtatcgtagt aagctcgtac ccaggtggag acgttctcca gcgcctggcg
     6121 ataagtctct tcctggtgac gcggtacagc ttgtgctgcg accagcaggc gagagcgaat
     6181 attttcgcgc agatagatat cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg
     6241 acggcgaatc gtaatgaagt tgtccataaa gttctgccag cttttttgca gattgatacg
     6301 ccattcgctg atggaactgg aaagctcttc accgtcggaa tccatcggcg aaccatcgct
     6361 gtcattatcg gccagacgca ggttatctac ctgatttgaa agctgattaa gcttaaggat
     6421 gatgccgtca taatccacct gcgaaactgc agaaaggctg gcgatatcat cggtaattgc
     6481 ccgacgaacg gtaatcagac tcgggtcatt catatccgcc aggctggcat ctgcactttt
     6541 cagcaacgct gcagcggtcg tgacgtcctg atcgctccac agcttccgtc cggcgagttt
     6601 caccagaaaa tcggcctgag ccagcagcca ggttttagca tcgctgccgg aaatggtggc
     6661 gaccttttgt tggacttcat ccaactgttt tgccagcgtt tcttgctgac gattcgcctg
     6721 cttaagctgt gcagcttgtt gcttaataat gccttccagc tcggcttttt ggctctcctg
     6781 ggctttttgc aatgccgtca gttggttagc cagggcatcg ctggtggcgg tctgattgac
     6841 ggcctgttgt ttaccccagc catacaaacc gacgcccgcc gccagagcaa tagcgatagc
     6901 caccgcgctg agaatcaatg cggtattgtt cttactcttt ttttctgttg cgacaggttg
     6961 tgacgtggtg tccacggcct ccctggtctc ttcaaccacg gcggaggttt tttcttgttc
     7021 cgtcattatg gcttcctgtt atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc
     7081 agcgttatcg gcgaccttaa tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg
     7141 ctcactgacg accaatagtc gacagtgtag taaccagtgc tcacgatacc attgtgggat
     7201 cagcgaccag agttgctgca acatttcacc gctggtaaca acgaccatcg tcacctcgcg
     7261 ggattgccag cgcatcgctt cttctgcacc atcgtaatgg attgcgcatc gttgataaca
     7321 ttcacaaaaa gtgacctcag caccgcgcgc tgtcagggta tccccaatta gctcacgacc
     7381 gccattgcca cgtaatatca gcgcacgttt gcccgcaata ttttgtaatt caggtaattg
     7441 tagcaagact tcgctgattt cccgatcctg cgggtagaga atcttctgtc cgcttacggt
     7501 atgtagcgcc agtgcggtgg tgcgtccaat ggcgaaataa tcaggtagtc ggggccattt
     7561 acgatcttgc tgatgcagct gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa
     7621 caacagatcg ctctccccca gcgccgccag ttgatcagca agttgcggta attgtcgacc
     7681 cggagaaaac tcaatcagcg gaaaatgcca ggccacctgc cccagtgtgc gcagacggct
     7741 cactaactct tctccagcgg gagacgggcg ggtgaccagg atactcatgc cggggcgtct
     7801 ccgttataga cttcagcgag gatctcgcgc gcgccgttat tcagtagctc ttctgccagc
     7861 gaaatcccca tttgttcggc attttgcggc gcaccgcggc gttcaccgcg aataatctgc
     7921 gaaccgtccg gcgcgccgac cagcgcacgc agccagattt caccatcaat aagctcggcg
     7981 tagctaccaa ttggcacctg acatccacct tcgagacggg tattcatggc gcgttctgcg
     8041 gtaacgcgca gtgcagtttc gtggtgattc agcgcggcaa gcagctcgcg agtgcgcgta
     8101 tcatcaaggc ggcattcaat accgactgca ccttgtccta ccgccggaag agaaatctcg
     8161 ggtggtaacg cggcgcgaat gcgggactcc agacctaaac gttttagtcc ggctacggca
     8221 agaatgatgg catcgtattc gccgttatct agtttgctca ggcgagtgcc gacgttgccg
     8281 cgcagggagc ggataatcag atccgggcgg cgttcagcca gttggcactg gcgacgtaaa
     8341 ctggacgtcc cgacgatact gcctgccggt aacgcatcca gactgtcata attattggac
     8401 acaaaggcat cgcgaggatc ttcacgctca caaatagtga ccaaccccag accttgcggg
     8461 aattcaaccg gcacatcttt cattgaatgt acggcgatat cggcgcgatt ctccaggagc
     8521 gcgacttcca gctcttttac aaataagcct tttccgccta ctttcgccag cggcgtatca
     8581 agaatcacat cgccgcgcgt caccatcggt accagttcaa ccaccaggcc cggatggctc
     8641 gccatcaact tgtctttgac atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg
     8701 gcaattctta aaacattgtc taacatgctt gttaccgtca ttatcatccg tggtccatcc
     8761 taacatcctt gccagagtga tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaatcg
     8821 taaaatgagg tctggcagtg gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt
     8881 acagagaata aacggtgcta cacttgtatg tagcgcatct ttctttacgg tcaatcagca
     8941 aggtgttaaa ttgatcacgt tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc
     9001 gcgaaaagtg gtaacggtta cctttgacat acgaaatatc ccgaatgccg cgtgttaccg
     9061 ttgatgttgg cggaatcaca gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct
     9121 ctatattgag actctgaaac agagactgga tgccataaat caattgcgtg tggatcgcgc
     9181 gcttgctgct atggggcctg cattccaaca ggtctacagt ctactgccga cattgttgca
     9241 ctatcaccat ccgctaatgc cgggttacct tgatggtaac gttcccaaag gcatttgcct
     9301 ttacacgcct gatgaaactc aacgccacta cctgaacgag cttgaactgt atcgtggaat
     9361 gtcagtacag gacccaccga aaggtgagct tccaattact ggtgtataca ccatgggcag
     9421 cacctcgtcc gtagggcaaa gttgttcctc tgacctggat atctgggtct gtcatcaatc
     9481 ctggctcgat agcgaagagc gccagttact acaacgtaaa tgtagtctgc tggaaagctg
     9541 ggccgcctcg ctgggtgtgg aagtcagctt cttcctgatt gatgaaaacc gcttccgtca
     9601 taatgaaagc ggcagcctgg ggggcgaaga ttgtggctcc acccagcata tactgctgct
     9661 tgacgaattt tatcgtaccg ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt
     9721 gccgtgcgac gaagaagagc attacgacga ctatgtgatg acgctttacg cgcagggcgt
     9781 gctgacgcca aatgaatggc tggatctcgg tggcttaagc tcgctttctg ctgaagagta
     9841 ctttggtgcc agcctttggc agctctacaa gagtatcgat tccccataca aagcggtact
     9901 gaaaacactg ctgctggaag cctattcctg ggaatacccg aacccacgtc tgctggcgaa
     9961 agatatcaaa cagcgtttgc acgacggcga gattgtatcg tttggtctcg atccatactg
    10021 catgatgctg gagcgtgtta ctgaatacct gacggcgatt gaagatttta cccgtctgga
    10081 tttagtacgt cgctgcttct atttaaaagt gtgcgaaaa
//
LOCUS       DAFATA010000137        10090 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFATA010000137 DAFATA010000000
VERSION     DAFATA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.138"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..2353
                     /locus_tag="LGJ20_004270"
     CDS             <1..2353
                     /locus_tag="LGJ20_004270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK2388265.1"
                     /translation="LFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRT
                     PLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISD
                     VTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLD
                     PQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLEN
                     LLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRY
                     LRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSL
                     QAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLA
                     GVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEYA
                     AESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEALW
                     ALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPNL
                     AGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSMED
                     SGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNLL
                     PWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTTA
                     LRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(2404..2751)
                     /gene="grxD"
                     /locus_tag="LGJ20_004271"
     CDS             complement(2404..2751)
                     /gene="grxD"
                     /locus_tag="LGJ20_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBK2388266.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            3086..3901
                     /gene="mepH"
                     /locus_tag="LGJ20_004272"
     CDS             3086..3901
                     /gene="mepH"
                     /locus_tag="LGJ20_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBK2388267.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            4029..4610
                     /gene="sodB"
                     /locus_tag="LGJ20_004273"
     CDS             4029..4610
                     /gene="sodB"
                     /locus_tag="LGJ20_004273"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBK2388268.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(4756..5925)
                     /locus_tag="LGJ20_004274"
     CDS             complement(4756..5925)
                     /locus_tag="LGJ20_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388269.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(6091..6180)
                     /gene="ynhF"
                     /locus_tag="LGJ20_004275"
     CDS             complement(6091..6180)
                     /gene="ynhF"
                     /locus_tag="LGJ20_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBK2388270.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            6479..7504
                     /gene="purR"
                     /locus_tag="LGJ20_004276"
     CDS             6479..7504
                     /gene="purR"
                     /locus_tag="LGJ20_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBK2388271.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(7501..8433)
                     /locus_tag="LGJ20_004277"
     CDS             complement(7501..8433)
                     /locus_tag="LGJ20_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2388272.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            8546..9757
                     /locus_tag="LGJ20_004278"
     CDS             8546..9757
                     /locus_tag="LGJ20_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBK2388273.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
BASE COUNT         2465 a         2673 c         2529 g         2423 t
ORIGIN      
        1 tttgtttgaa ccagaaaagt tgctgcaaat tgtccgcgag gcggtaggca gctcggcact
       61 tttcgccgcc cgttttcgcg aatgcgccgc gcgggcatta ttaatgccgg ggcgcactcc
      121 gggccatcgc accccgcttt ggcaacaacg gctgcgcgcc agtcagttgc tggaaatcgc
      181 tcagggatat ccggattttc cggtcattct cgaaaccctg cgcgaatgtc tgcaagatgt
      241 ttatgatctt cccgcactgg aacgtttgat gcgtcgcctg aacggtggcg aaattcaaat
      301 atccgatgta acgaccacta cgccctcgcc tttcgccaca agtttattgt tcggctatgt
      361 cgcggaattt atgtaccaga gcgacgcccc gctggcagag cgccgggcat ccgtactgtc
      421 gctggacagc gagttactgc gcaatctact cggacaggtc gatccggggg aattactcga
      481 cccgcaggtc attcgccagg tggaagaaga gttgcaacga ctggctcctg gcagaagagc
      541 gaaaggtgaa gaaggattgt tcgacctgct gcgcgaactg gggccaatga ccgttgaaga
      601 cctggcgcaa cggcatacag gcagcagtga agaggttgcg tcgtatctgg aaaatcttct
      661 tgcagtaaaa cgaatcttcc cagcgatgat tagcggacag gagcgtcttg cctgtatgga
      721 tgatgccgcc aggctgcgtg atgccctcgg cgtacgacta ccagagtcat tgccagagat
      781 ttatttacat agagtcagtt acccgcttcg cgacctcttt ctgcgctatc tccgggctca
      841 tgctctggtc acggctgaac aactggctca tgagtttagt ctcggtattg ccattgtcga
      901 agagcagctt cagcaactgc gtgaacaggg tctggtgatg aatctgcaac aagacatctg
      961 ggtgagcgat gaagtatttc gtcgtctgcg tttgcgctcg ctgcaagccg ccagagaagc
     1021 gacgcgtccc gttgcagcca cgacctatgc gcgattgctg ctggaacgtc agggcgtatt
     1081 acccgccacc gatggtagcc cggcgctctt tgcctcaaca tcgccaggcg tttatgaggg
     1141 cgtagatggc gtgatgcggg tgatcgaaca gcttgccgga gtcggtttac ccgcctcact
     1201 ctgggaaagc cagatcctgc ctgcccgcgt acgcgactat tcgtcagaaa tgctcgatga
     1261 attactggca accggtgcag ttatctggtc ggggcaaaaa aaactgggtg aagatgacgg
     1321 cctggtggca ctgcatctac aggaatatgc tgcagaatcg ttcactcccg ccgaagcgga
     1381 tcaggcgaat cgttcggcgc tgcaacaagc gatagtcgct gttctggctg acggaggagc
     1441 ctggtttgca caacaaatca gccaacggat acgcgacaaa atcggcgaat cggttgatct
     1501 ctctgccctg caagaggcgt tatgggcgct ggtctggcaa ggcgtcatca ccagcgacat
     1561 ttgggcaccg ttacgcgccc tcacccgcag cagttccaac gcacgcacct caactcgccg
     1621 cagtcaccgg gctcgtcgtg gacgtcctgt ctatgcgcaa cccgtctcgc cgctggtatc
     1681 ttacaacaca ccaaatctgg ctggacgctg gtcgttattg caggtggagc cactaaacga
     1741 taccgaaagg atgctggcgc tggcggaaaa tatgctcgac cgctacggca tcatcagtcg
     1801 tcaggcggtg atagccgaaa atatccctgg cgggtttcca tcgatgcaaa cgctttgtcg
     1861 aagtatggaa gactccgggc gaattatgcg aggtcgtttt gtagaaggtc tgggtggcgc
     1921 gcaattcgct gaacgtctga ctattgaccg attgcgcgat ctggcgacac aagccacgca
     1981 aacgcgccac tatacaccag tggcgctctc tgccaacgat ccggctaatg tgtggggaaa
     2041 tcttctgccc tggcctgcac atccggcaac gctggttcca acgcgtcggg cgggtgcgct
     2101 ggtggtcgtt tctggcggca aattgttact ctatctggcg caaggtggca aaaaaatgct
     2161 ggtctggcag gaaaaagagg aattactcgc cccagaggtt ttccacgcgc tgactaccgc
     2221 actgcgtcgc gaaccacggc tgcgctttac gctaacagaa gtgaatgatc taccggtccg
     2281 gcaaacgccg atgtttacgc tgctgcgtga ggcgggattt tcaagttcgc cacaagggct
     2341 ggattgggga tagagaaagg actgacggat gcccgttcgc atccgtcagt attgcaggac
     2401 ggattattcc gcgtccggct cttcagactt gtatttagcg gcagtttctt tgatcagctg
     2461 ctgcagttcg ccacgctgat acatttcgat cacgatatca caaccgccga ccagctcgcc
     2521 gtcaacccac agttgcggga aggtcggcca gttagcatat ttcggcagtt ccgcacgaat
     2581 gtccggattc tgcagaatat caacataggc aaaacgttcg ccacatgcgg caagcgcctg
     2641 gactgcctgg gcagagaaac cgcagctcgg cagtttcggt gaacctttca tgtacagcag
     2701 gatcgggttt tcagcaatct ggcgttggat tttttcgata gtggtgctca ttgtcttgct
     2761 tcctcaaact tctttacggc agtaatctga cattgtagcg ggtcagtgcg gcatcggaaa
     2821 ataacatttt catcacgctt ttgctatttt atccctttgc tctatttgtt gcatttcaaa
     2881 tattcgtttc ttctctgatg cgcggcaaac tggcgtattt ataaccattt ttagctttca
     2941 ctgctgcatt tttttgcgct cgccaacgaa acgtattttt taacaataaa agctattaac
     3001 tttctcttct tctatgcatt agaatcatca agttttgtaa atcagacgca ggcatgatag
     3061 acctgcctta acagagggac gctcagtggc gcggataaac cgtatttcga tcacgctctg
     3121 tgctttactt tttaccaccc tgcctttaac gcctatggcc catgcttcaa agcaagccag
     3181 ggagagttct gctaccactc atatcaccaa aaaagcagat aaaaagaaaa gcacggcaac
     3241 caccaaaaag acccagaaaa cagcgaaaaa agccgccagt aaaagtacga ccaaaagcaa
     3301 aaccgcttct tccgttaaaa aatcttccat taccgcttct aaaaacgcca aaactcgcag
     3361 caaacacgcc gtcaataaaa cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa
     3421 gggctataaa tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaactc ttgccgacgc
     3481 gcacaaagcg aaggtgcaaa aagctacgaa agtggcaatg aataaactga tgcagcaaat
     3541 tggtaagcca tatcgttggg gtggcagctc accgcgtacc ggttttgatt gcagcggcct
     3601 ggtttattac gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat
     3661 gtatcacctg cgtgatgcag cgccaatcga acgtagtgaa ctgaaaaacg gcgacctggt
     3721 ctttttccgt actcagggac gcggcacagc cgatcatgtc ggcgtgtatg tcggcaacgg
     3781 caaatttatt cagtcaccgc gcacaggtca ggaaattcaa atcacttctc tcagtgaaga
     3841 ctactggcag cgccactatg ttggcgctcg tcgggtaatg accccaaaaa cacttcgcta
     3901 aaactttacc ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt
     3961 tgctacccta tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga
     4021 gagtagcaat gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc
     4081 acatttctgc ggaaactatc gagtatcact acggcaagca tcatcagact tatgtcacta
     4141 acctgaacaa cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa gagattattc
     4201 gcagctctga aggtggcgta ttcaacaacg cagctcaggt ctggaaccat actttctact
     4261 ggaactgcct ggcaccgaac gccggtggcg aaccgactgg aaaagtcgct gaagctatcg
     4321 ccgcatcttt tggcagcttt gccgatttca aagcgcagtt tactgatgca gcgatcaaaa
     4381 actttggttc tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt
     4441 caacctctaa cgcgggtact ccgctgacca ccgatgcgac tccgctgctg accgttgatg
     4501 tctgggaaca cgcttattac atcgactatc gcaatgcacg tcctggctat ctggagcact
     4561 tctgggcgct ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg
     4621 caaatgcagc attgcctgat gcgcttcgct taccatgcca acgcggatga ggcgttaaca
     4681 gcgcattcgg caagataaat cgcacgttgt cagcaactgt aacgcagaag gttatccttc
     4741 tgcgtttttg tttaattagc tgttagcaac gcaaactgtt tcaggttgtt ttctggctga
     4801 cataaacacc agcaataatg ccagtcccgc gacaatcgct cccatcaccg gcacaaagct
     4861 gtatcccagc ccagcggaaa ttaccgcacc accagcagct gctcccagcg catttccaag
     4921 attaaaggca ccaatattga ctgatgaaga cagacccggc gcttcactgg cgacacgcat
     4981 cacgcgcatc tgtaacggcg gtacgaccgc aaaggttgct gcgccccaca ccaccatgct
     5041 aatagctgcg ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcaa
     5101 caacaacaaa aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag
     5161 atagttaccg atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacactgg
     5221 tgttgcgtgg gtaatacttt gtagtaccgg agagatatag gtgtagagag taaacattgc
     5281 accagctccc agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc
     5341 cagctctttt ttcacttcag gtcgtgcccc tgcaccacct ttaggtaatg agaagaacag
     5401 acttaccatt gaaatcactc ccagccccgc cgttgccaga aatgacatcc gccagccgat
     5461 ggtttcaccc aaccaggtcg ccgccggcac gccaccgata tttgccaggg ttaaccccat
     5521 aaacatagtg gcaactgcgc tggcctgttt atgttttggc accacgcttg cggccacgac
     5581 tgaacccaaa ccaaaaaatg ctccgtgatt caggctggtc aaaatgcgtg aaagcatcag
     5641 ggtcatataa tccggcgcga tggcggaaag tacgttgccg agcgtgaaaa ttgccatcag
     5701 gaaaatcaac gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc
     5761 aaccattacg ccaactgcat aggcactgat taacattccg gcagcgggaa tcgagacatc
     5821 cacaccgcgc gcaatgacgg gcaacaaacc cattggcgag aactccgttg tcccgatacc
     5881 aaacgcgcca atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct
     5941 cttcgcgtca ttacgcgata ttcattaaag tggcgaaagc atgacagcaa tcacaaaaaa
     6001 atgaaaataa caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg
     6061 cgagggagaa cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca aacccacggc
     6121 tacgatcaaa ccgaggacga taatcgttgt taccagtgaa aatttaaggt cggtgctcat
     6181 caagttttct ccttttttat taccacacaa aaagtgatat tacgcatttt tacacactgt
     6241 gatgaaaaaa tctcccgtca tttataatga taagtgtttt taccacttcc ccttttcgtc
     6301 aagatcggcc aaaattccac gcttacacta tttgcgtact ggccattgac cccttcctga
     6361 cgctccgtgt cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag gtgtgtaaag
     6421 gcaaacgttt accttgcgat tttgcaggag ctgaagttag ggtctggagt gaaatggaat
     6481 ggcaacaata aaagatgtag cgaaacgagc aaacgtttcc actacaactg tgtcacacgt
     6541 aatcaacaaa acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg cagcgattaa
     6601 agaattacac tactccccta gcgcggtggc gcgtagcctg aaggttaacc acaccaagtc
     6661 tatcggtttg ctggcgacca gcagcgaagc ggcctatttt gccgagatca ttgaagcggt
     6721 tgaaaaaaat tgcttccaga aaggttacac cctgattctg ggtaatgcgt ggaacaatct
     6781 tgagaaacag cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt
     6841 gatgtgttct gagtacccag agccgttgct ggcgatgctg gaagagtatc gccatatccc
     6901 aatggtggtg atggactggg gtgaagcaaa agctgacttc accgatgcgg tcattgataa
     6961 cgcgttcgaa ggcggctaca tggccgggcg ttatctgatt gaacgcggtc accgcgaaat
     7021 cggcgtcatc cctggcccgc tggaacgtaa caccggcgca ggccgccttg ccggttttat
     7081 gaaggcgatg gaagaagcga tgatcaaggt gccggaaagc tggattgttc agggtgactt
     7141 tgaacccgaa tctggttatc gcgccatgca gcaaatactg tcgcagccgc atcgccctac
     7201 tgccgtcttc tgtggtggcg atatcatggc aatgggcgca ctttgtgctg ctgatgaaat
     7261 gggtctgcgc gtcccgcagg atgtttcgct gatcggttat gataacgtgc gcaacgcccg
     7321 ctattttacg ccggcgctga ccacgatcca ccagccaaaa gattcgctgg gtgaaacagc
     7381 gttcaacatg ctgttggatc gtatcgtcaa caaacgtgaa gaaccgcagt ccattgaagt
     7441 gcatccgcgc ttgattgaac gccgctccgt ggctgacggc ccgttccgcg actatcgtcg
     7501 ttaatcaccc gttgcgggag tctcttccgg ctcccgcagc cactccttat tcagcgtctc
     7561 actatcgccg agatactcaa gcagccaggt taacgcaggc gacatatcat tttgctgcca
     7621 tgtcagacaa catgccgaat ccgggaaggg gttttccaat tcaagtgcta cccacttccc
     7681 ctcattaagc cacggtttgg cgaaatgtgt tggcaccatc cctatgcata atcctgccga
     7741 gatacaggtt gccgatgatt cccagtcagg cacgacaact cttttttggt tatccagcaa
     7801 ccaggtaata cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt
     7861 gcgcaacgta tcatcgctga atgggccatc catcaacgcc agcgggtggt ggctggcaac
     7921 aacgcaactc cagcttagca tccccatatc ccggaaggca taacgaccgc ctaccggaat
     7981 cgcgcgtgtt gcgccaatcg ccagttccac gcgcccgtcg gaaagcgcat cccagacacc
     8041 gttgaacact tcctgaaaga caagaagttc gacatcatca aaatggcgat aaaaatcaac
     8101 gatcatctgc cgtgtacgtt ctggcctgac aatattatcc actgcgatag ctaactgacc
     8161 gcgccagccg ttcgctatct gctgacattg ctggcgggtg atctgcattt ttttgacaac
     8221 agagcgccct tctttgagaa accacgctcc agcagcggtc agctccacat cacggtgccg
     8281 tcgttcaaag agcggcaccg ccagccactc ttccagctga cgcacggtat agctgaccgc
     8341 agaaggaacg cgatgcagct cctgtgccgc agcgctaaaa ctaccattac gcgctaccgc
     8401 atcaacaact tcgagtgaat attctgacca catagtctgc ctgcaaaatt tttgaaacca
     8461 gtcatcaaat attaccgttt cacaacacta atttcactcc ctacactttg cggcggtgtt
     8521 taattgagag atttagagaa tatacatgca acctgggaaa agatttttag tctggctggc
     8581 gggtttgagc gtactcggtt ttctggcaac cgatatgtat ctgcctgctt tcgccgccat
     8641 acaggccgac ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc
     8701 cggttttgcc gcagcccagc ttctgtgggg gccactctcc gaccgttatg gtcgtaaacc
     8761 ggtgttatta atcggcctga caatttttgc gttaggtagt ctggggatgc tgtgggtaga
     8821 aaacgccgct acgctgctgg tattgcgttt tgtacaggct gtgggtgtct gcgccgcggc
     8881 ggttatctgg caagcgttag tgacggatta ttatccttca cagaaagtta accgaatttt
     8941 tgcgaccatc atgccgctgg tgggtctatc tccggccctg gctcctctgt taggaagctg
     9001 gctgctggtc catttttcct ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt
     9061 gctgattctg cctattttct ggctcaaacc cacgacgaag gcccgtaaca atagtcagga
     9121 tggtctgacc tttaccgacc tgctacgttc taaaacctat cgcggcaacg tgctgatata
     9181 cgcggcctgt tcagccagtt tttttgcatg gctgaccggt tcaccgttca tccttagtga
     9241 aatgggctac agcccggcag ttattggttt aagttatgtc ccgcaaacta tcgcgtttct
     9301 gattggtggt tatggatgtc gcgccgcgct gcagaaatgg caaggcaagc agttattacc
     9361 gtggttgctg gtgctgtttg cagtcagcgt cattgcgacc tgggctgcag gcttcattag
     9421 ccatgtgtcg ctggtcgaaa tcctgatccc attctgtgtg atggcgattg ccaatggcgc
     9481 gatctacccg attgttgtcg cccaggcgct gcgtcccttc ccacacgcaa ctggtcgcgc
     9541 cgcagcgttg cagaacactc tacaactggg tctgtgcttc ctcgcaagtc tggtagtttc
     9601 ctggctgatc agtatcagca cgccattgct caccaccacc agcgtgatgt tatcaacagt
     9661 agtgctggtc gctctgggtt acatgatgca acgttgtgaa gaagttgact gccagaatca
     9721 tggcaatgcc gaagtcgctc atagcgaatc acactgatct atatcgatat acttatactt
     9781 aggctgctaa caaaattttg ttgtatgatt gaaattagcg gcctatacta atttcgagtt
     9841 gttaaagcta cgataaatat tatgttttta cggggacagg atcgttcccg actcactatg
     9901 gatggtcatt tcggcaaggg ttcctccttt ccctctgttc tacgtcggat tatagactcg
     9961 cggttttttc tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc
    10021 tgatcaccga ccagtgatgg agaaactatg agttcatcgt gtatagaaga agtcagtgta
    10081 ccggatgaca
//
LOCUS       DAFATA010000138        10029 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFATA010000138 DAFATA010000000
VERSION     DAFATA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.139"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            265..1098
                     /gene="fdhD"
                     /locus_tag="LGJ20_004279"
     CDS             265..1098
                     /gene="fdhD"
                     /locus_tag="LGJ20_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBK2388274.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1251..2291
                     /locus_tag="LGJ20_004280"
     CDS             1251..2291
                     /locus_tag="LGJ20_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBK2388275.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(2341..4089)
                     /locus_tag="LGJ20_004281"
     CDS             complement(2341..4089)
                     /locus_tag="LGJ20_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBK2388276.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGYNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIHEE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4089..5159)
                     /locus_tag="LGJ20_004282"
     CDS             complement(4089..5159)
                     /locus_tag="LGJ20_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK2388277.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            complement(5149..6600)
                     /locus_tag="LGJ20_004283"
     CDS             complement(5149..6600)
                     /locus_tag="LGJ20_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBK2388278.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(6611..7057)
                     /locus_tag="LGJ20_004284"
     CDS             complement(6611..7057)
                     /locus_tag="LGJ20_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK2388279.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLRRGDADQVFALLNQTLSS"
     gene            complement(7370..7684)
                     /gene="rhaM"
                     /locus_tag="LGJ20_004285"
     CDS             complement(7370..7684)
                     /gene="rhaM"
                     /locus_tag="LGJ20_004285"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBK2388280.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(7694..8518)
                     /gene="rhaD"
                     /locus_tag="LGJ20_004286"
     CDS             complement(7694..8518)
                     /gene="rhaD"
                     /locus_tag="LGJ20_004286"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBK2388281.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            complement(8693..9952)
                     /gene="rhaA"
                     /locus_tag="LGJ20_004287"
     CDS             complement(8693..9952)
                     /gene="rhaA"
                     /locus_tag="LGJ20_004287"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBK2388282.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
BASE COUNT         2456 a         2529 c         2631 g         2413 t
ORIGIN      
        1 agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg
       61 ctgacctgca tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc
      121 tgcgtgtgca gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat
      181 tggctcaatt tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt
      241 gtgttcttat ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg
      301 acaaatatca caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca
      361 cggttagatg aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat
      421 gtggtgatga tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc
      481 gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat
      541 ggtcttgaag tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc
      601 cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc
      661 ggaaaaccgg tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat
      721 gcattacgtc atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc
      781 gctgcctgga tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat
      841 gtggcgctgg acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt
      901 gcggtactgg tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc
      961 gtagagattt tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt
     1021 tgtaatctga cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg
     1081 cagcgtttga gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat
     1141 tcatttcgtg aattttatta ttttatttat aaataaaacg cgtgtagtgg cgttttcttt
     1201 tttcatttat tatgttcact ctcttatcta tatatttata aggcaattaa atgaaaagga
     1261 atttattatc ttccgctatt atagtcgcca tcatggccct cggtctgacg ggttgtgatg
     1321 ataaaaaagc cgaaacagaa acgcccccgc ctgccaatag tcaacctgcc gcaccagctc
     1381 ctgaagctcc cgttgcaaaa gcagaagcta aacctgaaac acctgcgcaa ccggtggtcg
     1441 atgaacaagc ggttttcgac gaaaaaatgg atgtctatat caagtgctat aacaagttac
     1501 agatccctgt acagagcagt ctggcgcgtt atgctgactg gctgaaagat tttaaacagg
     1561 ggcctaccgg tgaagagcgt actgtttatg gcatctacgg cattagtgaa tccaacctcg
     1621 ctgagtgtga aaaaggcgta aaaagtgctg tggcgttaac gcctgcgctg caacctattg
     1681 atggcgtggc ggtgagttat atcgatgctg ccgtggcgtt gggtaatacc attaacgaaa
     1741 tggataaata ttacacccag gaaaattata aagacgatgc gtttgcgaaa gggaaaacgc
     1801 tgcaccagac attcttaaaa aatctggaag cctttgaagc tgtagcagaa tcttatcatg
     1861 cggcgattca ggaaattaac gataagcgcc agcttgcgga actgaaaaat attgaagaaa
     1921 gagaagggaa aacattccac tattactctc tggcagtcat gatttcagcg aaacaaatta
     1981 ataacctgat atcgcaagat aagtttgatg cagaagcggc aatgaagaaa gtgtctgaac
     2041 tggaaacgct ggtggcgcag gccaaagaag cggataaagg cggcatgaat ttctcgttta
     2101 ttaattcggc aggccagtat caactcgagg ctaaaaaata cgttcgccgc gtcagagata
     2161 aagtcccgta ctctgactgg gataaagagc aactgcagga cgcaaactca agctggatgg
     2221 tcgacgactc tttcccgaga gcattacgcg agtacaacga aatggttgat gactataata
     2281 gcctgcgtta aacgtttttg atgataaatg cagtgtcgga tgcggcgcat ccgacacagt
     2341 tcaccccttg tgaagtaact ccataaatgc ctcatatcct gttaacccgg caataacctc
     2401 tgccggatgc tgacacaata cgcttgccag atagctaaat atttttagct catgacgcgc
     2461 atcggcggcg gcgcaggcaa tcaacacatg gttaatgatt tcgttattca cctcgatcgg
     2521 gtgggcgagg gtaataaaaa atccacgaaa acgtcgctcc tgttcgctcc agcaatgtgg
     2581 gatggcgagg cgattaacaa tcaggttttc accttcgcct tcgcgggcga tgatgcgttg
     2641 tgcttcatcg gcggtaatat ggtgttgtgc cactaattgc gcacaaattt gccgggtaat
     2701 atgttgccag ctttcaccgc gtacattcga ataatgaaaa cttccggggg cagagaaaaa
     2761 acgctccggt tgctggcgaa taaatgccgt cgccagaaaa tgttttattt gttcgatacc
     2821 ggcagccgta atgatatttt ttacggtgat gtaattattt accgcgtctt ccagtaaata
     2881 atggctattg ttaatgatca ataacggctc aatctcttcg tgaatggcaa caagttcgct
     2941 taagctacgg gcaataatta cccgacaatt taaaacatcg cgctcaatgg cgagctgatt
     3001 aatagtggca atcgcgttct ggtccgagag caaaatgatc ggctggcgtt cgttttgatg
     3061 ccgctccagc gcacaggcaa aatacaaacc gatcagatcg ctgtcgaaaa ggggaatatc
     3121 gagctgttca cgcagtagcg taataaagtg cagactcata tcaaaggccg caggccaggc
     3181 ggctttcagg ttattcatgc tgctttggcg gtgctcagca atccacactg gtgcggcgag
     3241 gcaacgtaat aaatgcccgg taatgttctc aaccagctgc gtatcaataa tattcaatga
     3301 cgcgatgccc ggcacccttt gcagcaaccc cagcacatga tcggctgaaa ttacctgcgc
     3361 ttgctgatgc tgtttctcta tcaaacccgt cagggttctt acagcgttat aaccaagaaa
     3421 cagaccgcta tgttcaacga tttgttttat ctcgtcaccg ggatattgcg gccactcatc
     3481 ggtcagttga ttacgcaggg cgtaaatcgc cagaatcagg ctggagagat aatcaccctg
     3541 catgagcggc cagcggtgtt ggttgtcgca ggccgtggat aaatgctgaa ggttgtctcg
     3601 tgttatgccc gccagcggaa ttaaaaacgg atctttgcgc agcaagttcg ccagcaagat
     3661 aacgcgtttc tcttctgtct catcaatgaa atggcccaaa ccagggcggc tggccaggca
     3721 acaagtgcgt tcataacgct gttttaaccg gggcagacgc tctgctaccc acgtttctgg
     3781 caaattaagc gccgaggcga gttgcgcacg gggagtgaaa gtattcagta ataacagcgc
     3841 cagcagccga tcgtcgttat cgtgcttttg cagcaactga aaaaagctgc ggcgctcgaa
     3901 gatttccagc tgatagcccg cactaccact ggcgaaaatg cgggctttgc cgttaagggt
     3961 gaagttgaga tagtcaatat cacgcaggat ggtcctgcct gaaacgccag tctgttgcgc
     4021 cagctctcca ggcgtgcgcg gctgttgctc cagcagatcg acaatcttta actggcgttc
     4081 gtttagcatt aatccacctg acggaactgg ctaaacgcgt tgactttctc cgcagttaag
     4141 gtcgtcagaa aatcccgtat cagcgtgagc agagcatcgt aatcggcttt tgaaatcatg
     4201 ccgctgttag cgtgcagata acgggttggc agacacagcg cgacaaccgg acgcccgccg
     4261 cccatcacgt tgtagcgccc gccatccgtc gctccggttt tcatggtgga aaattgcagc
     4321 ggtaaatcgt tatgtgcggc acagtttttt aacactgcta ccagtttctg gttggggaag
     4381 tagcgcttgt caaacagcat cagccccggc ccctggccca gtttcagcgg gtatttaatg
     4441 ttatcaatgc ccggaacatc acctgcgaca gcggtatcca gcacaatcac cacatccggt
     4501 ttaatgtgtt ccgccgaggt ttgtgccccg cgtagcccaa cttcttcttc cacactgcca
     4561 acgccgtaca gcgtaatttc tgggttattt actgtctgta gtagctccgc catcatcgcg
     4621 cagccgatgc ggttatccag cgcctttccg actactttat cttcgcccca gcaggcaaaa
     4681 ttggcttccg ggctaataaa atcgccaatt tcaacgccgc gcttttccgc ttcttcgcga
     4741 ctgttcgcgc caatatcaat gaacatctca tcaaatgaca gcggttgttg cttttgcttt
     4801 tccgttaacg catgaggcgc gacggaacca atcacaccag ggattttaaa tcccttgtgt
     4861 gtgcgtatgg ttacccggtg gttgagcatc gactgattcc accagccgcc aatggtggtg
     4921 aagcgcagaa aaccgctctc gtcgatgtgg gtgaccataa agccgacttc atccatatgc
     4981 ccgacaacgg caacttttgg acctttattc cccttacggg caacaaagct gcccagacca
     5041 tcaaaggtga tttcattaac gcaaggttcc agcgtgttta tcaggatgtc gcgaacttcc
     5101 tgttcatcgc cgctgacggc gctggcttcg cacaactgtt gcagtaactc aatgttcatg
     5161 gacaagctcc tgttgcgcgg ctttcgcttt gcggcgtaac cacaatcctt tcaggacgat
     5221 aatgaatgaa atgtttagcg cgaggccaat cgccagcacc agataaaacg aacctaccgg
     5281 cgacatcagg ccgatcagcg ggtcaaagat acccaaacca ggtgccagac gtttgattcc
     5341 gaaagcgatc accagcacgc cggtaatacc gcccgccagc gtattggcgg taatcatcgg
     5401 taacggagct gccagagcgt aaggaatagc aggttcggtt gcgactgtgg caccgaccac
     5461 aatggcacta ctggcagcct ctttttcctg tcgggtaaac agtttgggcg caatgaacgt
     5521 cgcaagaccc gccgcgacgg gcggcatcag cgcgacaacg ccgacgatgg cgtaccagtc
     5581 ataaatgtgt ttttccagca gtgagaagca gaagaaccag gcggttttat tgatggggcc
     5641 gcccatatcg aacgccagca tcgcaccgac cagaaacgct gccgcaaatt tcattgatgg
     5701 aggaatggtg ttcaggaagt gcagcaaccc gcccatgagg tcggacatta ccggtccgat
     5761 aacgtagtaa gtgagcacgc cgaaaaccag cagagtaacg aacgggatca gcattgaacc
     5821 gagcagcggt tgtaatgctt tgccaaggcg cactttacgg aaccagaaga cgaaatagcc
     5881 aatcgccagc cccagcacca ccgcgccgag gaatcccgca cctgactggg tgccgagcag
     5941 ggctttgtca ttcgccagat agcaaaccag aaacgccgga gcgaaagcgg gtttatcggc
     6001 aatcgacgag gcaatataag cgcccatgat ggggatcata aaggtgaagc cgagataacc
     6061 gatggattca accacccagg taaacgacgg cgcgcctttc gacatatcgg tatacggcag
     6121 gccgaactgc accagcatgt tggcaagcgc caccaggata ccgccaccga taacaaacgg
     6181 cagcgccgca gagacgcccg ccatcaggtg gctcattacg ctgccgcttt gtacttcctg
     6241 tttgccgagc ttcacgccgc tatctgcggc aaaaagctgc gagtttgttg gtaattcact
     6301 gaaaatctgg tcgatatttt tcaacgcctg ggagatggca atctcataaa ccttcttccc
     6361 ggcaaaccgc gcccggtcat cgccgctcag gccacgcccg gtagcgagaa tgacgtaatc
     6421 ggcagcggca atctcttcgc tggataagcg gttttcaacg ccactggacc cttgagtttc
     6481 cacttttatg gtatggccga gagaacgggc tttctgttcc agggcttccg ccaccatgta
     6541 ggtgtgagcg atcccggcgg ggcagttggt gatcgcgaca atacgtaagg atgactccat
     6601 cgccacttcc ttatgagctg agggtttgat ttaacaaggc aaacacctgg tcggcgtcgc
     6661 ctcgtcgcag ttggtgaata aaatcctggt ggataatttt gcgacacagt gtaccgatga
     6721 ttttgacctg atcctcttcg ccgctttgcg gcacgccgag gcagatccag caattgacgt
     6781 cttcgccatc gctggctttc cagtcaatag cctgcgcttt gcgggcgaat aacacgaacg
     6841 gttgtttaac gcaggcgctt ttaccgtgcg gcacggcgat gccggaacca aatccagtag
     6901 agtgcatttt ttcgcgcagc agcagggttt gcaggaactg gtgtgagtcg gtgataaaac
     6961 cgttttgtaa cgccattgtc gccaactgct tcagaatgga ataagcgcca ttgccctgaa
     7021 tattcaggtc aatacagctt gcagtaagag ctgccattaa tcggactcca tgaaatatat
     7081 tttaatcaat tgtaatggtg gagcgattaa gtgatgagag gcgatatgtc ggtaaacaat
     7141 gacaatttga gacagagtga aaggtcagat ttgcggagta atgcacataa tggttattta
     7201 aataaaccac atgaatcatt aatgagttat tcattatttt tgtgatttac tcacaatgca
     7261 tcggggaaat ataagttacc gggatatatt aactgaaggt gtaatatgtt tatttttcaa
     7321 atcgaaatta aaaatattgt gccggaggcg tctccggcac attaggcaat tacggcaggt
     7381 aaaacacttc ttgcagttcg ctactcaccg ggctgttatc cgggttcgcg ggcatgacgt
     7441 cggtcatata tttccaccaa cgttggcaaa catcagtact ggcaaccgca ttccagcgct
     7501 cttcagattc aatctctacc gtggcaaaca gcagattacg cgctttgtcg aggtaaatgg
     7561 cgtagttatg cgcaccgtga gatttcaaca ctgcttccag ttccggccag atggggttat
     7621 gccgacgctg atactcttcg tgagcgtcgg ggttaacctg catgacaaag gctttgcgga
     7681 tcatgtttgc tccttacagc gccagcgcac tggctagtgg tgtaacgccg aagcgcttgc
     7741 cgagcgctat caactcttca cggctgatgg tctgtttcat gccgcccatc gaataaacct
     7801 tcactaatac ttgtgctgat ttttctgcgg tgtcgattaa accgaatgtt tcatccagcg
     7861 tcggcccgct gccgaagacg ccgtggaagg accacaacac cagcgaatgt ttttgcatct
     7921 cttgtgcggt cgcctggccg atttcgtccg tgccgggcac catccacggc aaaatgccaa
     7981 cgccatccgg gaacactacc agacactcgg tgctgccttc ccacagttgg cgagtaaaga
     8041 ccgcggtgtc gttttccagt acataggtga gggcgatcag gttggtggcg tggcagtgca
     8101 tgatcacccg atctttaccg ttggtggctt taatgcgctc gcagtgggaa aggaagtgag
     8161 ccggaagttc ggaagtggga acggcttcgt tggttaaccc ccagagaata tggtagcccg
     8221 cgccgtcgct gtcgactttt acgacgccta agttagctgc aggatcaagc tggacgttac
     8281 ggaagaattt gccggagccg gtgacaataa acggcgtatt tgccagtaaa ggcatgggct
     8341 ggctgagcgg aatatagcgc ggttgtgggt ggaagttgtc gtgataaagc gcgatatcgg
     8401 cgtcatccag gcgcagcgtc aggttgccgc cgttgcgctc gtcccagcct ttcagccagg
     8461 catcggtggt ggctttgatc attccctgga caaaccagga ctgggtaatg ttttgcatgt
     8521 tctgtgttcc tgtaaattcg gtgttgtcag atgcacgacc cgtaggccgg ataaggcgct
     8581 cgtgccgcat ccggcaatca atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa
     8641 ctgttgccga cctgtaggcc ggataaggcg ctcgcgccgc atccggcagt gtttacccac
     8701 gctggctcaa aatcgctttc tcataagccc gcacgctctc cagccattcg ctacccgttg
     8761 gggtatcgtg acgttggcaa tacatttccc agaccgcctg ccacggcaac gatttctgct
     8821 cttccagcag tgccagacgc gcagtgtaat cgcccgccgc ttccaacttg cgcagctcgg
     8881 cggtaggttc cagcaacgca cgcagcaggg cttttttcat attgcgtgta ccaatgaccc
     8941 acgcggcaat gcggttgata gaggcatcga agaagtcaag gccgatatgc acccggtcaa
     9001 acagatcgtg acgcacaatc tcactggcaa ttgcctgggt ttcatcatcc agcagcacta
     9061 cgtgatcgct gtcccagcga accggacggc tgacgtgcag cagcaactgc ggcacataca
     9121 gcatggcggc ggaaatcttg tcggaaatca cttcagtcgg gtggaagtgc ccggcgtcca
     9181 ggcacagcgc agtctggcgg ctggtggcat accccatgta aaactcattg gagccaaccg
     9241 tgtagctctc tgcgccaatg ccaaacaatt tgctctcaac ggcgtcgata tggtgcgcag
     9301 ggtttagctt ctcgctgatc acctcatcca gtgctgccag cagacgctga cgcggggcga
     9361 gacggtcaac ggtgatatct ttcataccat ccgggatcca gatgttcatc accgatggtg
     9421 tgccgagttg ctcgccaaaa taggccgaaa cgcgacggct ggctttgcag tgatcaatcc
     9481 agaactggcg aatgctgtcg tcggcatggg aaagcgtaaa gccatcggcg cttagcggat
     9541 gcgaaaagca ggaggggtta aaatccagac cgagctgatt ggctttcgcc cattcaaccc
     9601 agtttttgaa gtgctctggt ttgatctgat cgcgcgagac tggcgtatct gattccagat
     9661 agatggcatg taaattaagc cgtttcggcc ccggaatcag ccgcatagcc tgttccagat
     9721 cggtacgtag ctcactggca ttacgcgctt tgcccggata attaccagta gcttgaatcc
     9781 caccggtcag cgaaccttcc gggttttcaa aaccggaaac atcatcgccc tgccagcagt
     9841 gcattgaaac gggtaaacga tcaagttggc gcagcgcctc ctcgacatca atccccaccg
     9901 ccgcgaaacg ctgtttcgcc agctcccagg cctgttccag ttgagtggtc atgcgcaaag
     9961 ctcctttgtc tgtcgtgtag agtgaatctg cgccacatag tgggcaattt cactgtcagg
    10021 attaggggt
//
LOCUS       DAFATA010000139         9949 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFATA010000139 DAFATA010000000
VERSION     DAFATA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.140"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(23..1567)
                     /gene="ilvA"
                     /locus_tag="LGJ20_004288"
     CDS             complement(23..1567)
                     /gene="ilvA"
                     /locus_tag="LGJ20_004288"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBK2388283.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(1570..3420)
                     /gene="ilvD"
                     /locus_tag="LGJ20_004289"
     CDS             complement(1570..3420)
                     /gene="ilvD"
                     /locus_tag="LGJ20_004289"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBK2388284.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(3485..4414)
                     /gene="ilvE"
                     /locus_tag="LGJ20_004290"
     CDS             complement(3485..4414)
                     /gene="ilvE"
                     /locus_tag="LGJ20_004290"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBK2388285.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(4434..4697)
                     /gene="ilvM"
                     /locus_tag="LGJ20_004291"
     CDS             complement(4434..4697)
                     /gene="ilvM"
                     /locus_tag="LGJ20_004291"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBK2388286.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(4694..6340)
                     /locus_tag="LGJ20_004292"
     CDS             complement(4694..6340)
                     /locus_tag="LGJ20_004292"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBK2388287.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQVRQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLCDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(6343..6393)
                     /locus_tag="LGJ20_004293"
     CDS             complement(6343..6393)
                     /locus_tag="LGJ20_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388288.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(6480..6578)
                     /gene="ilvL"
                     /locus_tag="LGJ20_004294"
     CDS             complement(6480..6578)
                     /gene="ilvL"
                     /locus_tag="LGJ20_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBK2388289.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            6931..8451
                     /locus_tag="LGJ20_004295"
     CDS             6931..8451
                     /locus_tag="LGJ20_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBK2388290.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(8476..8814)
                     /gene="maoP"
                     /locus_tag="LGJ20_004296"
     CDS             complement(8476..8814)
                     /gene="maoP"
                     /locus_tag="LGJ20_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBK2388291.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            8933..9772
                     /gene="hdfR"
                     /locus_tag="LGJ20_004297"
     CDS             8933..9772
                     /gene="hdfR"
                     /locus_tag="LGJ20_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBK2388292.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(9868..9943)
                     /locus_tag="LGJ20_004298"
     tRNA            complement(9868..9943)
                     /locus_tag="LGJ20_004298"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9908..9910),aa:Trp,seq:cca)
BASE COUNT         2339 a         2686 c         2572 g         2352 t
ORIGIN      
        1 aagcgctatc aggcattttt ccctaacccg ccaaaaagaa cctgaacgcc ggattattgg
       61 tttcgtcgtg gcaatcgtag cccagctcat ttagccgggt ttcgaaatcc ggttcatggt
      121 cgccgagttc gaacgccgcc agtacgcgcc cgtagtcggt gccatggctg cgatagtgga
      181 acaaagaaat gttccagtgc gtacccagcg tgttgaggaa gcgcagcagc gcgcccggtg
      241 attccgggaa ttcgaagctg tagaggcgtt cctgcaacgg atgtgacgga cgcccgccga
      301 ccatatagcg cacatgcaac ttcgccattt cgtcgtcgga gagatccacc acgctgtagc
      361 cgccgtcgtt gagcatctgc aaaatttctt tgcgctcttc gaggccgcgg ctcaggcgca
      421 cgccgacaaa gatgcaggcg tttttggcat cggcaaaacg gtagttgaac tcggtgaccg
      481 atcgcccgcc aagcagttgg cagaatttga ggaagctgcc tttttcttcc ggaatggtca
      541 ccgccaacaa cgcttcacgc tgttcgccca gttcgcagcg ttccgagacg tagcgcaggc
      601 cgtggaagtt cacgttggca ccggaaagaa tatgcgccag ccgttcgccg cgaatgttgt
      661 gctgggcgat atattttttc attcccgcca gcgccagcgc gccagagggt tccgccaccg
      721 cgcgcacatc ttcgaataaa tccttcatcg ccgcacagat cgcatcacta tcgacggtga
      781 tgatgtcgtc gagatactcc tggcataaac ggaaggtttc gtcaccgatg cgttttaccg
      841 ctacgccttc agcaaatagc cctacgcgcg gcagatcaac cggatgaccc gcatccagcg
      901 ctgctttcag gcaggcggag tcttccgctt ctacggcgat cactttgatt tgtggcatca
      961 gttgtttgat cagcaccgca acgcccgccg ccagaccgcc gccgccgact ggcacaaata
     1021 cgcggtcgag atgggcgtcc tgctggagca gttccagcgc cagcgtgcct tgcccggcaa
     1081 tcaccatcgg atggtcgaac ggcggcaccc aggtgaaccc ctgctgctgt gacagttcga
     1141 tcgctttagc tttcgcttcg tcaaagttcg cgccatgaag cagcacttcg ccgccgaatc
     1201 cacgtaccgc atcgactttg atgtcggcgg tggcggttgg cataacgatc agggccttca
     1261 cgcctaaccg cgcagaagaa aacgcgacgc cctgcgcgtg gttacccgca gaagcagtga
     1321 tcacgccgtg cgctttttgt tcttccgtca ggcccgccat catggcgtat gcgccgcgca
     1381 gcttaaagct gtgcactggc tggcgatctt cgcgcttcac cagaatgacg ttatcaagac
     1441 gcgacgacag tttttccatt ttttgtagcg gcgtaacctg cgccgcctcg taaaccggcg
     1501 cacgcagcac tgctcttaaa tattcggcac cttccggagc accggacagg ggttgcgagt
     1561 cagccattat taacccccca gtttcgattt atcgcgcacc gcacctttgt cggcgctggt
     1621 tgccaggctg gcgtaggcgc gcagggcaaa ggaaacctga cgttcacggt ttttcggcgt
     1681 ccaggctttg tcaccccgag cttcctgcgc ttcacgacgc gccgccagtt cggcatcgct
     1741 tacctgtaac tgaatgccac ggttcgggat gtcgatagcg atcaggtcac catcttcaat
     1801 caggccaatg ctgccgccgc ttgccgcttc cggtgagacg tggccgatgg aaagaccaga
     1861 ggtgccgcca gagaaacgac cgtcggtgat cagcgcacag gctttaccga gacccattga
     1921 tttcaggaag ctggttgggt agagcatttc ctgcatgcca gggccgcctt tcgggccttc
     1981 atagcggatc accaccacat cgcccgcgac aactttgcca ccgaggatcg cttctaccgc
     2041 gtcgtcctgg ctttcgtaca ctttcgccgg gccggtgaat ttgaggatgc tgtcatcgac
     2101 gcccgcggtt ttaacgatgc agccgttttc tgcgaaatta ccgtagagca ccgccaggcc
     2161 gccgtctttg ctgtaggcgt gttccagcga gcggatacag ccattggcgc gatcgtcatc
     2221 gagagaatcc caacggcaat cctgcgagaa tgcctgtgtg gtacgaatac ccgcagggcc
     2281 ggcgcggaac atatttttta ccgcgtcatc ctgggtcagc ataacgtcgt attgttccag
     2341 cgtttgcggc aacgtcaggc caagtacgtt tttcacatca cggttcagta accccgcgcg
     2401 atccagttcg ccgagaatac cgataacacc accagcacgg tgaacatctt ccatatggta
     2461 tttctgggtg ctcggcgcaa ctttacacag ctgtggaacc ttgcgggaaa gcttatcgat
     2521 atcactcatg gtgaagtcga tttccgcttc ctgcgccgcc gccagcaggt gaagtactgt
     2581 gttagtcgat ccacccatcg cgatatccag cgtcatggcg ttttcaaacg ccgccttact
     2641 ggcgatatta cgcggcagtg cactttcgtc gttttgctcg taataacgtt tggtcaattc
     2701 aacaatgcgt ttaccagcat taaggaacag ctgcttacgg tcggcgtggg ttgccagcag
     2761 cgagccgttg cccggctgcg acaggcccag cgcttcggtc aggcagttca ttgagttagc
     2821 ggtaaacatc ccggagcagg aaccgcaggt cggacacgcg gaacgttcaa cctgatcgct
     2881 ctgggagtca gatactttcg ggtctgcgcc ctggatcatc gcatcaacca gatcgagctt
     2941 gatgatctga tcggaaagtt tggttttccc ggcctccatc gggccgccgg aaacaaagat
     3001 caccggaata ttcaggcgca gggaagccat cagcatcccc ggggtgattt tgtcgcagtt
     3061 agagatgcag accatggcgt cggcgcagtg ggcgttgacc atatactcaa cggaatcagc
     3121 gatcagttcg cgagatggca gtgaataaag catccccccg tggcccatgg caatcccatc
     3181 atccaccgca atggtgttga actctttggc aacgccgcca gccgcttcaa tttgttcggc
     3241 gaccagttta ccgagatcgc gcagatggac gtgacccggt acaaattggg tgaacgagtt
     3301 cacaaccgcg ataatcggct tgccaaaatc ggcgtcggtc attccggtgg cgcgccacag
     3361 cgcacgagca cccgccatat tacgaccatg agtggtggtg gcggaacggt acttaggcat
     3421 actttattta ctcccagtgt ctgtctcgta aatgggacgg tgcgtgccgt cccatttttt
     3481 gtatttattg attaacttga tctaaccagc cccatttatc ttcggtttcg ccagtgaaga
     3541 ggccgaagaa ggcttgctga atgcgtttgg taaccgggcc acaacggcct tcgccaacct
     3601 gaataccgtc tacgctgcgc actggcgtga tttctgccgc agtaccggac ataaacactt
     3661 catccgccag gtacagggat tcgcgcgaca gaacctgctc acgcacttca attcccagct
     3721 ctttcgccag tttgatgatg gcatcacggg taatacccgg cagcgcggag gaggtgaacg
     3781 gtggggtgaa cagcacacca tctttcactt caaacaggtt ttcgcctgcg ccttcagaga
     3841 tataaccgtt cacatccagc gcgatacctt cctgataacc gtggcggcgc gcttcgctac
     3901 ccaccagcag ggaagagagg tagttaccac cggcttttgc cgcggttggg atggtgtttg
     3961 gtgctgcgcg gttccaggag gaaaccatcg catcgatccc ctgctccagc gcttctgcgc
     4021 ccagatacgc tccccacggg aaagcagcga taatcacgtc ggttgagtat cccgctggcg
     4081 ggtttactcc catgccaaca tcaccgacga agatcagcgg acggatatag gcgctggtga
     4141 gattattttt gcggatcacg tcacgacaag cttccatcag ctcatcaatg ctctgcgaaa
     4201 ccgggaagcg atagattttg gcggagtcat gcagacgctg catatgctca cgatggcgga
     4261 atacaaccgg tcctttgtgc gagtcgtagc aacggatgcc ttcaaaaacc gaagtgccat
     4321 agtgcagcgc gtgcgacatc acatgcacct tcgcgtcttc ccagcgaacc atctccccat
     4381 tgaaccaaat gtaatcagct ttcttcgtgg tcatttttat attccttttg cgctcaggcg
     4441 cggatttgtt gtgatgtggt tgtgctctgg cagatggcaa cgtgtgcgac gtccaccagt
     4501 ttatttaact gactaaacag taagtcgacc gaccgtgggc tggcaacggt caattcgata
     4561 tttatatttt gtgcatcgct ggcggcggcc atattcattg agcagacgtg gaaaccacga
     4621 tgacgcacca cgcgtaaaac acgttctaag gtttccggat tgaagcgagc cgatacattg
     4681 acctgatgtt gcatcatgat aatttctcca acatttctga attactggca ccaggcggca
     4741 ccagcggcca gacgttctca agttcgtcga ttgagacatg aagcaggtat ggcccatcac
     4801 tgttcagcat ggtgtcgagt gccgcttcaa cctggtcttt acgggtgatg tgttggccag
     4861 ggatgccgaa ggcgctggct aacatgagaa aatcggggtt atcggtaagg gtggtttcgc
     4921 tatatcgttc ctggaaaaac agttgctgcc attgtcgaac catccctaac cgttggttat
     4981 cgagtaagac gattttcaac ggtaactgct tacgttttac ggtgcccagc tcttgtacat
     5041 tcatcatgaa agagccgtca ccggagatac agacgacggt atcgttcggt cgcgcgactt
     5101 gcgcgccaac cgccgccggt aaaccgaaac ccatggtgcc taagccgctg gaggtgatga
     5161 aattttccgg gcgagtatgg gcgatgtgct gcgcagccca catctggtgc tgccccacat
     5221 ctgtggtcac gacgcaatcc gcaggtttac gatccgacag ttgttttaac aacaatggcg
     5281 cgtagatagc gtcaccggga tggtcgtaac gccaggcatg ttcatcacac agctgcgcgc
     5341 agtgtagctg ccagtcattg atatttaacg gctgctgtaa tgctggtaac agagcattta
     5401 aatcaccttg taatgccaca tgtgcctgac gcagcttgtt catttctgcc gggtcgatat
     5461 ccatatggat aacactggcg tgtggtgcga aggtgttcag tttgccggtc acccggtcat
     5521 caaaacgtgc acccacggcg atcagcaagt cgcactcctg caccgcgaag ttcgccgctt
     5581 tggtgccatg cattcccagc atgcccagat agtacggata atctgcttca actgcgccca
     5641 gccctttcag cgtgcaggtg gcaggcattt ttgtggtagc gagaaattct cgtaaagcag
     5701 gaactgcctg cgccataccc acgccaccac caacgtacag catcggtttt tgcgcttttg
     5761 ccagcatctg gcgcacttgc tcgacttcgg catgtgggaa agtcacttcg ttttcaacgg
     5821 tggtgaacca cggttccagg tcgccgctgg ctaactggat atcttttggg atatcgacca
     5881 gaaccggacc aggacgacct gagctggcaa cgtcgaatgc ttcagccata atgcgcggca
     5941 actcttccag cgactgcacc agaaagctgt gcttggtaca ggctaacgac aatcccagga
     6001 catccacttc ctgaaatgcg tcagtgccga taaacggtgc ggacacttga ccggtgatgg
     6061 caacaacagg gatagaatct aacagtgcgt ccgcaagccc ggttatcagg ttggttgcgc
     6121 ccggaccaga cgtggcgata catacgccag ttttgccggt agcacgagca taaccgatag
     6181 ccgccattgc cgcaccctgc tcatgtcggc acagcaagtg ctccacgccg ccgtcataca
     6241 atgcatcgta aaccggcata attgcgccac ccggataacc gaaaacggtg ttcacaccct
     6301 gtgcccgcaa cgcatgtacc acccactgtg cgccattcat agttagttcc ccgtcctgaa
     6361 tcttgagaaa cagaattttg tgctgttatt cattgtctgc tcctcggtta tgtttttaag
     6421 gtcaaaaaaa acccccggac ctttcggtgc gggggtctta gttcgttaag gcttgatctc
     6481 taagcctttc ctcgtccaag tgcagccccg cacggtggga taataatcac caccacgcta
     6541 atcacgacca agctaatcac tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt
     6601 tcgaaggaat gcctaaagag ttaccataga ttttgcgaat agcacaagat attttttggc
     6661 cgttttttat gatggcttta ataatttcaa tagaaattgt tgttttttaa aaagaaaata
     6721 gtgagtgaaa aaaattcaat ttcagtacga atgttgttcc ctccctcgta gttcatcctt
     6781 tgtccgctgc atttttgcga gcatcattcc accgccagaa caactcaact tcacttgcac
     6841 aaataaacag gaatcagcca ctaaaattca cgaattagcg ggctacagca gcgatatctt
     6901 atgcacataa tggtgactca aggagggctt atgtcactgt caattgttca tacccgcgca
     6961 gccctgggag taaatgcgcc cccaatcact gttgaggtac atatcagtaa aggtctaccc
     7021 ggcttaacga tggtgggctt accagaaaca actgtaaaag aagctcgcga tcgcgtgcgc
     7081 agcgccatta tcaatagcgg atatgaatat ccggcgaaaa aaatcaccat caacctggct
     7141 ccagccgatc tgccaaaaga agggggacga tatgatttac ctatcgccat tgcgttgctg
     7201 gcggcctcag aacagcttac agccaataag ttagatgaat atgaattagt cggagaactg
     7261 gcgcttacag gcgctctgcg tggcgttccc ggcgcaatct ccagtgcaac tgaagctatt
     7321 aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag atgaagtggg gctaattaac
     7381 ggtgaaggat gcctgatagc cgatcatctg caggctgtct gtgcgtttct ggaaggtaag
     7441 cacgctctcg aacgcccgaa accaactgat gcagtatccc gggcgctaca acatgatctc
     7501 agtgatgttg tcggtcagga gcaaggaaag cgaggactgg aaattaccgc cgctggcggg
     7561 cacaaccttt tactgattgg gccgccggga acaggtaaaa caatgctcgc cagccgtatt
     7621 aatggcctgt tgccagattt aagcaatgaa gaggcactgg agagcgctgc gatattaagt
     7681 ctggtaaatg ctgaatcagt acaaaaacaa tggcggcagc gcccgttccg ctcaccgcat
     7741 cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt
     7801 tcgttggcgc ataacggcgt gctttttctt gatgagctac ctgaatttga acggcgtaca
     7861 ctggatgcct tgcgagagcc gattgaatcc gggcagatcc atctttcacg cacacgagcc
     7921 aaaataacct atccagcccg tttccagctt gttgcggcga tgaatcccag ccctaccgga
     7981 cattatcagg gaaaccataa ccgctgcacg ccagaacaga cattacgtta tctcaaccgg
     8041 ctctcggggc cctttctcga ccgcttcgat ctctcactgg agatcccatt accacccccc
     8101 ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa acaacgcgta
     8161 atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg gctggatagt
     8221 ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt ggaaggaaca
     8281 ctgatccatc tggggttatc gattcgtgcc tggcagcggt tattgaaagt tgcacgaacc
     8341 attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga ggcagttagc
     8401 tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata aaaaaagggc
     8461 atttcgccct ttttattaat catcagaatc ggtgtagtct tcagcacctt caacctgcgg
     8521 tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca tatacttgga
     8581 ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg ctacgaagag
     8641 tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga aggcataacc
     8701 atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag agaatccacg
     8761 tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg ccatcctgca
     8821 cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg cttgacagag
     8881 tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca ctgtggatac
     8941 ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc gagcggctga
     9001 atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg aaaatcaact
     9061 gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa
     9121 actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca aggaggtggc
     9181 gcatacctca cgacataacg agttttctat cggtgccagc gcctcattgt gggaatgtat
     9241 gcttaatcag tggctgggac gtttgtatca aaatcaggat gcccatacag gcttacagtt
     9301 cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac gccagcttga
     9361 tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt tgctgggata
     9421 tttcacttta gcgctttata ccagtgcccc ttcaaaacta aagggagatc ttaattatct
     9481 gcgacttgag tgggggccag attttcaaca gcatgaggca ggtttgatcg gtgctgacga
     9541 agtgcccatt ctgacaacca gttctgctga actggcacag caacagattg cgatgcttaa
     9601 tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt
     9661 cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa
     9721 tacgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt aatatgaatg
     9781 gctggcaagg atgccggtag aaggatttac ttcggagagg gttatttcag ataaaaaaaa
     9841 tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa ctcccaacac
     9901 ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattag
//
LOCUS       DAFATA010000140         9948 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFATA010000140 DAFATA010000000
VERSION     DAFATA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.141"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..696)
                     /locus_tag="LGJ20_004299"
     CDS             complement(<1..696)
                     /locus_tag="LGJ20_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBK2388293.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLT"
     gene            1308..4013
                     /gene="malT"
                     /locus_tag="LGJ20_004300"
     CDS             1308..4013
                     /gene="malT"
                     /locus_tag="LGJ20_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBK2388294.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICHDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDILLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(4056..5072)
                     /gene="rtcA"
                     /locus_tag="LGJ20_004301"
     CDS             complement(4056..5072)
                     /gene="rtcA"
                     /locus_tag="LGJ20_004301"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBK2388295.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRSTVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(5076..6302)
                     /gene="rtcB"
                     /locus_tag="LGJ20_004302"
     CDS             complement(5076..6302)
                     /gene="rtcB"
                     /locus_tag="LGJ20_004302"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBK2388296.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            6491..8089
                     /gene="rtcR"
                     /locus_tag="LGJ20_004303"
     CDS             6491..8089
                     /gene="rtcR"
                     /locus_tag="LGJ20_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBK2388297.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(8071..8829)
                     /locus_tag="LGJ20_004304"
     CDS             complement(8071..8829)
                     /locus_tag="LGJ20_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBK2388298.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(8846..9676)
                     /gene="glpG"
                     /locus_tag="LGJ20_004305"
     CDS             complement(8846..9676)
                     /gene="glpG"
                     /locus_tag="LGJ20_004305"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBK2388299.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(9721..>9948)
                     /gene="glpE"
                     /locus_tag="LGJ20_004306"
     CDS             complement(9721..>9948)
                     /gene="glpE"
                     /locus_tag="LGJ20_004306"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBK2388300.1"
                     /translation="MGHAVQAFHLTNDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQY
                     LLQQGYDVVYSIDGGFEAWQRQFPAEVAYGA"
BASE COUNT         2389 a         2763 c         2515 g         2281 t
ORIGIN      
        1 agtcagatca aacggatgcg cgtgcgtcgc ctgccacaga cgcagcggct gcgccacgcc
       61 attacgatag ccgacaacgg ggagatccca cgcttgaccg gtaatggtaa actccggctc
      121 ccagcgtccg tctttcgtca ctttaccgcc aatccctacc tgcacatcca gtgcttcgtt
      181 gtggcggaac cacgggtagt tactgcgatg ccagtcatcc ggcgcttcaa cctgtttgcc
      241 atcgacaaaa gactggcgga acaaaccata ttgatagttc agaccgtaac ccgtcgcaga
      301 ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc agacgtccca gaccaccgtt
      361 acccagcgcc gggtcgatct cttcttccag caggtccgtc agattgatgt cataagcctt
      421 caacgaatcc tgtacatcct gataccagcc gagattcaac aggttgttgc ccgtcaggcg
      481 accaatcaaa aactccattg agatgtagtt aacatgtcgc tgattcgcca ccggcttggc
      541 gaatggctga gcacgcagca tttcggccag tgcttcactc actgctagcc accactggcg
      601 aggagtcatt tcagccgcag aatttaagcc ataacgctgc cactgacgtg aaagcgcttc
      661 ctgaaattgc ttatcgttaa aaataggttg tgacatagga gttccacttt tcttagattt
      721 tcaacacaac gttatcgcta gtttgccagg ctcgatgttg accttcctca tcctgcgggg
      781 gattaggcag ggaggagttg cggggatgag caaggaaatg tgatctcaac cacttaaagc
      841 tagtgcaaac cacaggatta gcagcaaatc aatgcaatac agcgcagaaa atctgtatct
      901 aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga aggtaactct ataaattaag
      961 taaaggagtg aaacagtttc ataagtaaaa tatccagtgt gctccatctc attcttaata
     1021 gatttattaa gatcatcttt ttagatggca ctttcatcag gaatgaagaa gaaacccttg
     1081 cttaaatgaa tctgatgaac ataagggaaa ccagtattca cgctggatca gcgtcgtttt
     1141 aggtgagttg ttaataaaga tttggaattg tgacacagtg caaattcaga cacataaaaa
     1201 aacgtcatcg cttgcattag aaaggtttct ggccgacctt ataaccatta attacgaagc
     1261 gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg attaactatg ctgattccgt
     1321 caaaactaag tcgtccggtt cgactcgacc ataccgtggt tcgtgagcgc ctgctggcta
     1381 aactttccgg cgcgaacaac ttccggctgg cgctgatcac gagtcctgcg ggctacggaa
     1441 agaccaccct catttcccag tgggcggcag gcaaaaacga tatcggctgg tactcgctgg
     1501 atgaaggtga taaccagcaa gagcgtttcg ccagctatct cattgccgcc gtgcagcagg
     1561 caaccaacgg tcactgtgcg atatgtgaga cgatggcgca aaaacggcaa tatgccagcc
     1621 tgacgtcact cttcgcccag cttttcattg agctggcgga atggcatagc ccactttatc
     1681 tggtcatcga tgactatcat ctgatcacta atccagtgat ccacgagtca atgcgcttct
     1741 ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt gtcacgcaac cttccgcaac
     1801 tgggcattgc caatctgcgt gttcgtgatc aactgctgga aattggcagt cagcaactgg
     1861 catttaccca tcaggaagcg aagcagtttt ttgattgccg tctgtcatcg ccgattgaag
     1921 ccgcagaaag cagtcggatt tgccatgacg tttccggttg ggcgacggca ctacagctaa
     1981 tcgccctctc cgcccggcag aatacccact cagcccataa gtcggcacgc cgcctggcgg
     2041 gaatcaatgc cagccatctt tcggattatc tggtcgatga ggttttggat aacgtcgatc
     2101 tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg ctcaatgaac gatgcactca
     2161 tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgcct cgaagagatt gagcgtcagg
     2221 ggctgttttt acagcggatg gatgataccg gcgagtggtt ctgctatcac ccgctgtttg
     2281 gcaacttcct gcgccagcgc tgccagtggg aactggcggc ggagctgccg gaaatccacc
     2341 gtgccgccgc agaaagctgg atggcccagg gatttcccag cgaagcgatt catcatgcgc
     2401 tggcggcagg cgatgcgctg atgctgcgcg atattctgct taatcacgcc tggagtttgt
     2461 tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa ggccctgccg tgggacattt
     2521 tgctggaaaa tccgcagtta gtgttattgc aggcgtggct aatgcaaagc caacatcgct
     2581 atggcgaagt taacaccctg ctagcccgtg ctgaacatga aatcaaggac atcagagaag
     2641 gcaccatgca cgcagaattt aacgctctgc gcgcccaggt ggcgattaac gatggtaatc
     2701 cggatgaagc ggaacggctg gcaaaactgg cactggaaga gctgccgccg ggctggttct
     2761 atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct gcactgcaaa ggcgaattga
     2821 cccgctcact ggcgctaatg cagcaaaccg aacagatggc acgccagcac gacgtctggc
     2881 actacgcctt atggagttta atccagcaaa gtgaaattct gtttgcccaa gggttcctgc
     2941 aaaccgcgtg ggaaacacag gaaaaagcgt tccagctgat caacgagcaa catctggaac
     3001 agctgccgat gcatgagttt ctggtgcgca tccgagcaca actgttatgg gcctgggcac
     3061 ggctggatga agccgaagcg tcggcgcgta gcgggattga agtcttgtcg tcttatcagc
     3121 cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg ctcgctggcc cgtggtgatt
     3181 tagataacgc ccgtagccag ctgaaccgtc tggaaaacct gctggggaat ggcaaatatc
     3241 acagcgactg gatctctaac gccaacaaag tccgggtgat ttactggcaa atgaccggcg
     3301 ataaagccgc cgctgccaac tggttgcgtc atacggctaa accggagttt gcgaacaacc
     3361 acttcctgca aggtcaatgg cgcaacattg cccgcgcaca aatcttgctg ggcgagtttg
     3421 aacctgcgga aattgttctc gaagaactca atgaaaatgc ccgcagtctg cggttgatga
     3481 gcgatctcaa ccgtaacctg ttgctgctta atcaactgta ctggcaggcc ggacgtaaaa
     3541 gtgacgccca gcgcgtgttg ctggacgcat taaaactggc gaatcgcacc ggatttatca
     3601 gccattttgt catcgaaggc gaagcgatgg cgcaacaact gcgccagctg attcaactca
     3661 atacgctgcc ggaactggaa cagcatcgcg cacagcgtat tctgcgagaa atcaatcaac
     3721 atcatcggca taaattcgcc catttcgatg agaatttcgt tgaacgtctg ctgaatcatc
     3781 cggaagtgcc agaactgatc cgcaccagcc cgttgaccca acgtgaatgg caggtcctgg
     3841 ggctgattta ctccggttac agcaatgagc aaatagcggg tgaactggaa gtggcggcaa
     3901 ccaccatcaa gacgcatatc cgcaatctgt accaaaaact cggcgtcgcc caccgtcagg
     3961 ctgccgtgca gcacgcgcag aaactgctga agatgatggg ttatggggtg tgagtttagc
     4021 cggataatgc attggatccg gtttacatct ttacatcatt caatactcac ccgcgttacg
     4081 ccatctgttt ctatcaaacc aaaccgcacc ggcaagaaac gctccaccac cgcgatattg
     4141 gtcagcagat ggcatgaagg atgggcgacc ttaaattctc ccgcgcccgc cagcgccatc
     4201 ggcagtacca gttggtcggc gagatattcc cccaccgccg ccgggcttgc caggtagcgt
     4261 ttcacctctt tcactaactg cgcggctacc acctcggcac tgacgcgctt ttcaccgacg
     4321 acgaaaaagc gttcagtgat attttcactt tcgacttcca gcgagacggt attacccggc
     4381 ccctggtcgc gcggcaggtt atgaatattc tgttcatgca gggaaaaact ccccgccagt
     4441 gtagcgattt cacgctcagc aacatggcgc ggcacaccag ctaatagaac ttctccacgc
     4501 atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt taaacgatgc cacgggcgag
     4561 acttccgttg ccaccacgcc gcctccggca ggataaaaac cgtgacgtaa cagcgtggtt
     4621 tgctgatgaa ttcctatttt cgccagcagc ggctccagca cccggcggat aaaatccgca
     4681 ggcggggccg acgggttatc ggtgcctccg ctcacttcaa cacgcgaagg tccatcggca
     4741 aaccacagcg cgggcagcac cgtttgcagc accagcgtac agcttccggc actaccgata
     4801 gcaaagcggt actcgccgcc gcgcacggtg ccaggccgga agaccagacg ctgcgacccc
     4861 agctccgcgc cttccaccgt tgacctacaa atttccgcag ccgcttttac cgcggtcaga
     4921 tgctggcgca acagccccgg tttcgcccgc ccggcacgaa tgctggtgat ggtaaatggc
     4981 tggccggtta tcatcgacag gctcagcgcc gagcgcagga tctgcccgcc accttcgccc
     5041 tgtgcgccat ccagcgcaat catccttttc atcatttatc cttttacgca caccacctga
     5101 cgcagggtat agataacttc caccagatcg ctttgtgccg ccatcaccgc atcaatatct
     5161 ttatacgcca tcgggatttc gtcgatcact tcggcatctt tacggcattc cacatgcgcg
     5221 gtggcacgaa tttgatcttc cacgctgaac agttttttcg ctttagtacg gctcattacc
     5281 cgcccggcac cgtggctgca cgaacagaac gactcttcat ttcccagccc acggacgata
     5341 aagctttttg ctcccatcga accgggaata attccatatt gaccagcacg cgcagacacc
     5401 gcgcctttac gcgtcacgta gatctcttca ccaaagtgct gttctttttg cacatagttg
     5461 tggtgacagt tgatctcttc catcgccagc gtttgtggct gtctgaccgt tttctgcgta
     5521 atgctctgca acgccgttac cacgttttcc atcatcgcat cgcggttaag gctggcgaaa
     5581 agctgcgccc aggccacggc tcttaggtaa tcatcaaagt attccgtacc ttccataaag
     5641 tacgccaggt cgcgcgatgg caacgtttca agctggtcct gcatctcttt ttgtgccaaa
     5701 tcgataaagt aagtcccgat ggcgttacca attccgcgtg aaccggagtg cagcataatc
     5761 cacacctggt ctgactcatc aaggcagatt tcaataaagt ggttacccgt tcccagcgtt
     5821 cccaggtgtt tatagttgtt agtgttaagg aaacgcggat atttttgcgt taaccactga
     5881 taaccggctt caagctcagc ccatttcgca tcgacgttaa caggcgggtt ttcccatgcg
     5941 cctttatcac gtttacaacg gccagtggta cgcccgtgcg gcacggccat ttcaatcgcc
     6001 tgacgcagct ctgccaggtt ttcaggcagg tcttccgccg ttaacgcggt acgcagcgcg
     6061 ttcattccac agccaatatc cacgcccacc gccgccggaa taatcgcccc tttggtcggg
     6121 atcacgctac caatggtgga acctttaccc aggtgtacat caggcattac cgcaatatgt
     6181 ttgaaaataa acggcatctt cgccgtatta ataagttgct gacgcgcatc ggcttctacc
     6241 ggcacgcctt tggtccacat ttttaccggg gcattttcag tggtcagtaa ttcgtaattc
     6301 attttgtttt tctcttttcg ttgtttgctg tcgtgataat tgcaaccgtt gtgccagaaa
     6361 attaaataag cagattaatt ttttaattca ttgtttttaa ataggttaaa tctttacgtc
     6421 cgtaaacgga gattttccac aaaaccaatt caccgataat gaaatatcgt cttttataag
     6481 gatatctaag atgcgtaaaa cagtggcttt tggctttgtc ggtaccgtgc tggattatgc
     6541 cggacgcggc agtcagcgct ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga
     6601 atcgttagtc atcgatcggc tggaattgtt gcacgacacc cgctcgcgct cgctattcga
     6661 aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat
     6721 tgaactgcat aacccgtggg atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg
     6781 tggttacgag tttcagccag aaaaagaaga ctatttaatt cacatcacca ccggcaccca
     6841 cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt tacctgcccg cccggctgat
     6901 acaatcttca cctccgcgca aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat
     6961 cgatctcgat ttaagccgtt ataacgccat cgccagccgc tttgccgagg aacgccagca
     7021 aacgcttgat tttcttaagt ccggcattgc cacgcgtaac ccccacttca accgcatgat
     7081 tgagcagatc gaaaaagtgg cgatcaaatc ccgcgcgccg attctgctta acggtccaac
     7141 cggcgcgggc aagtcatttc tggcgcgacg catcttcgag ttaaaacagg cgcggcatca
     7201 gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg cgcggcgata ccgccatgtc
     7261 gacgctgttt ggtcatgtaa aaggcgcgtt taccggggcg cgggaatctc gtgaaggttt
     7321 attacgcagc gccaacggcg gaatgttgtt tcttgatgag attggcgaac tgggcgcaga
     7381 cgaacaagca atgctgctaa aagccattga agagaaaacc ttttacccgt ttggcagcga
     7441 tcgccaggtg agcagtgatt ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct
     7501 ggttgccgaa ggcaaatttc gcgaagatct gtacgcgcgg atcaatctct ggaccttcac
     7561 cctgccgggt ctacgccagc gccaggaaga tattgaaccg aaccttgatt atgaagtgga
     7621 gcgccacgcc acactcactg gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg
     7681 gttggctttt gcgacctctc cccaggcaac atggcgcggt aactttcgcg aactttctgc
     7741 cagcgtcacg cggatggcca cctttgccac tagcggacgc atcactctgg acgtggttga
     7801 agatgagata aaccgtctgc gctataactg gcaggagagc cgcccctccg cgcttacggc
     7861 gttgctgggc gcagaggcag aaaacatcga cctttttgac aggctgcaac tggaacacgt
     7921 gatagctctc tgtcgccagg caaagtcgct ttccgctgcc ggacgcctgc tttttgatgt
     7981 ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg
     8041 ttttggtctg acgtgggaag ccgtgcagga tcagcacagc tccagttgaa tatgatggtc
     8101 cgtcagcacc tgcattacgc ttactggcgg cggggcgtcg gtgtagacgg catctaccat
     8161 gctgatgctg cccatattga ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag
     8221 cataacgtgg cgcgagttct caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa
     8281 ctccagcagc gagccgtcgc tatcgatgcc gcttatcccc agaatgccga aatcaaggcg
     8341 gaactgggag ataaaatcga gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa
     8401 ttcgccaccg gcgagaataa tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt
     8461 gagattgttg gtgacaatac gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc
     8521 cggcgtggtg ccgatatcga taaacagtgt cgagccgttg gggatttgct ccgccacttt
     8581 gcgggcaatc ctctcttttt cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt
     8641 aaccgaactg gaaggcagcg ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc
     8701 cagctcattg aggtcgcggc gaatagtctg cgggctgacg gagaaatgct ctaccagctc
     8761 ttcagtactg acataaccct gctgtttaac cagttcgata ataccgttgt gacgttgtgt
     8821 ttgtttcatt tataaatccc tggaattatt ttcgttttcg cgcattgagc gaatcaacaa
     8881 aagccatcgc taaacccacg gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc
     8941 caaacaaatc aaaccatccg gcgacaatcc agatcagcgc aaagataatt aacccacgtt
     9001 gcaggtaaat gccactttgc ggatcgcgtt caccacgtag ccagacgtag cccatcagcg
     9061 catacaccac gccagaaagc ccgccaaacc acggcccgct gaatttttgc tgcacatagc
     9121 cgcttaacag ggcgctaatg agagtaataa caattagctt accgctaccg aggcgttttt
     9181 ccaccgcacc gccgagatac caccaccaga gcaggttaaa gaggatatgc atcagcgaga
     9241 agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa tttcagtgtt ggatcgaatg
     9301 gccaggccag ccataacatc acttcctgat cgccgagaat ttgcatggcg ataaacacca
     9361 ccacgcaggc gatcatcacc acccaggtta ccggacctgc gcgttcacgc aaggcggcaa
     9421 agaaaggata acggcgataa tgcaggccac tgccggtatg gcctgcctgc cagctggccg
     9481 ccagataacg cggatctgcc gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct
     9541 cggcctggga ctcatccgcc agccagacat cgctttggtt atgttgttga atcgtgagga
     9601 taacaccctg cgtcgccatg taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag
     9661 aggtaatcat caacatcgtt gctgtcgctt attccacaca aaaggggaca gtataaagcg
     9721 ttacgcgccg tacgccacct ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc
     9781 aatgctatag accacatcgt agccctgttg cagcagatac tgcgccgcgc ctttgctgct
     9841 attgccgtga taacacatca ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc
     9901 gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca tgccccat
//
LOCUS       DAFATA010000141         9414 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFATA010000141 DAFATA010000000
VERSION     DAFATA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.142"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..164)
                     /locus_tag="LGJ20_004307"
     CDS             complement(<1..164)
                     /locus_tag="LGJ20_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388301.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNT"
     gene            317..976
                     /locus_tag="LGJ20_004308"
     CDS             317..976
                     /locus_tag="LGJ20_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBK2388302.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIDAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            1080..2054
                     /gene="ghrB"
                     /locus_tag="LGJ20_004309"
     CDS             1080..2054
                     /gene="ghrB"
                     /locus_tag="LGJ20_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBK2388303.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(2104..2814)
                     /locus_tag="LGJ20_004310"
     CDS             complement(2104..2814)
                     /locus_tag="LGJ20_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBK2388304.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3248..3538
                     /locus_tag="LGJ20_004311"
     CDS             3248..3538
                     /locus_tag="LGJ20_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBK2388305.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3819..4031
                     /gene="cspA"
                     /locus_tag="LGJ20_004312"
     CDS             3819..4031
                     /gene="cspA"
                     /locus_tag="LGJ20_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBK2388306.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(4219..4371)
                     /gene="hokA"
                     /locus_tag="LGJ20_004313"
     CDS             complement(4219..4371)
                     /gene="hokA"
                     /locus_tag="LGJ20_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBK2388307.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGCYELAAFL
                     ACNLKE"
     gene            complement(4697..6766)
                     /gene="glyS"
                     /locus_tag="LGJ20_004314"
     CDS             complement(4697..6766)
                     /gene="glyS"
                     /locus_tag="LGJ20_004314"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK2388308.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6776..7687)
                     /gene="glyQ"
                     /locus_tag="LGJ20_004315"
     CDS             complement(6776..7687)
                     /gene="glyQ"
                     /locus_tag="LGJ20_004315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK2388309.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7782..8081)
                     /locus_tag="LGJ20_004316"
     CDS             complement(7782..8081)
                     /locus_tag="LGJ20_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBK2388310.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFYADGHFLHLSMR"
     gene            8256..9251
                     /gene="wecH"
                     /locus_tag="LGJ20_004317"
     CDS             8256..9251
                     /gene="wecH"
                     /locus_tag="LGJ20_004317"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBK2388311.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
BASE COUNT         2332 a         2375 c         2333 g         2374 t
ORIGIN      
        1 gtattgctgt gaacctggtc gcgaaccgcg ctctgcaagg agttttccat tcctgtggct
       61 aacgcgccat gcgagctaaa cacggtttcg ccgtcggttt caaccagcat gggcccccaa
      121 tgggcggcag tcagaacgga atatcgtgag gatgagttgg ccaaaccgaa aactcctgaa
      181 aggttagatg tattgagcag ttaaatatta cttacaaatt tccgagtatt cccaggaata
      241 atcttcatgt tcacgcggca taatctcccg ccacggaacc cgtggcaaag aataaaaagg
      301 ttattaagga ttaacaatga agaaacgtgt ttatcttatt gccgccgtag tgagtggcgc
      361 tctggcggta tctggctgca caactaaccc ttacaccggc gaacgcgaag caggtaaatc
      421 tgctatcgac gcaggtctgg gctctctcgt gggcgcgggt attggtgcgc tctcttcttc
      481 gaagaaagat cgcggtaaag gcgcgctgat tggcgcagca gcaggcgcag ctctgggcgg
      541 cggcgtgggt tattacatgg atgtgcagga agcgaagctg cgtgacaaaa tgcgcggcac
      601 tggtgttagc gtaacccgca gcggggataa cattatcctc aatatgccga acaatgtgac
      661 cttcgacagc agcagcgcga ccctgaaacc tgcgggcgct aacaccctga ccggcgtggc
      721 aatggtgctg aaagagtatc cgaaaacggc ggttaacgtg attggttata ccgacagcac
      781 gggtggtcac gacctgaaca tgcgtctctc ccagcaacgt gcggattccg ttgccagcgc
      841 gttgatcacc cagggcgtgg acgccagccg catccgtact cagggccttg gcccggctaa
      901 cccaatcgcc agcaacagca ccgcagaagg taaggcgcaa aaccgccgtg tagaaattac
      961 cttaagcccg ctgtaatccc tttcatgcca ggtgatgcag aaatcacctg gcattcttca
     1021 gttttccttc atcatatttc aggctaaggt gatcgcctta tcagtgaatg gagagaagca
     1081 tgaagccgtc cgttatcctc tacaaagcct tacctgatga tttactgcaa cgcctgcaag
     1141 agcatttcac cgttcaccag gtggcaaacc tcagcccaca aaccgtcgaa caaaatgcag
     1201 caatttttgc cgaagctgaa ggtttactgg gttcaaacga gaatgttgat gccgcattgc
     1261 tggaaaaaat gccgaaactg cgtgccacat caacgatctc cgtcggctat gacaattttg
     1321 atgtcgatgc gcttaccgcc cgaaaaattc tgctgatgca cacgccaacc gtattaacag
     1381 aaaccgtcgc cgatacgctg atggcgctgg tgttgtctac cgctcgtcgg gttgtggagg
     1441 tagcagaacg ggtaaaagca ggcgaatgga ccgcgagcat aggcccggac tggtacggca
     1501 ctgacgttca ccataaaaca ctgggcattg tcgggatggg acggatcggt atggcgctgg
     1561 cacaacgtgc gcactttggc ttcaacatgc ccatcctcta taacgcgcgc cgccaccata
     1621 aagaagcaga agaacgcttc aacgcccgct actgcgattt ggatactctg ttacaagagt
     1681 cagatttcgt ttgcctgatc ctgccgttaa ctgatgagac gcatcatctg tttggcgcag
     1741 aacaattcgc caaaatgaaa tcctccgcca ttttcattaa tgccggacgt ggcccggtgg
     1801 ttgatgaaaa tgcactgatt gctgcattgc agaaaggcga aattcacgcc gccgggctgg
     1861 atgtcttcga acaagagcca ctgtccgtag attcgccgtt gctctcaatg gccaacgtcg
     1921 tcgcagtacc gcatattgga tctgccaccc atgagacgcg ttatggcatg gccgcctgtg
     1981 ccgtggataa tttgattgat gcgttacaag gaaaggttga gaagaactgt gtgaatccgc
     2041 acgtcgcgga ctaagccgcg actgcgtgaa gtaaagcccg ataatcgctc gggcttttac
     2101 tctttattgg gttgcagtaa ctgctgtagt ccaggcctga ttaaacgcct gatgttgtgc
     2161 cggtaatggc gcaatcagtt tgttatattc acttgcctgc tgtgaagtcg ggaactggat
     2221 gccatttgcg acaaagctca cctgagtacc ttgctgagca atgtagtctc caaccatcac
     2281 cagttgttgc gtgaaagttt gtgcagccgg aatcaacggt tgcaatgcat cagctggcgt
     2341 cgttaccact ttggtgaatg cctgatcaaa gaccggtttc aaatcatcgg tctgttttag
     2401 cgcagagtgc gcagcatcgg cctgcaactt cgcattttgc agttgttgcg ccagcacacc
     2461 cagcgaaccg ttcatttcac gcagcggacc gctttgcgta acgtaatcct gcggtacgcg
     2521 aattgcgtta acgctgtcta cgaccgggcg cagaccggaa tccatcgcct gattcacctg
     2581 ctgagaataa ccatacagaa tcgcgtaatc agagacaaaa ggaccaaact gttttttctg
     2641 atcggcagtc agggttggta gacgttcacc gctacgcatc actgtattct gcaggaagtc
     2701 gataaacgct ttacgctgat cgccttcttt atcaaaacac ccactcaggc taactaccat
     2761 taataacgcc gcaagaggcg caaaccagcg agagcaggac tttcctgtcg ccattttctt
     2821 actcctttca cccaaaaaaa gcgcacaacg acacacgcgt gtctgacgtg acaaggatag
     2881 tccaggacag gcttgcagga taccctttct acgtaatctt cctggtaaaa tcatggagaa
     2941 aatgaaacaa ggttaatttg ttgtttcatt gttaaaaaat gatagtgccg ttgctttttt
     3001 tgtagccaga gatttacgcc ttcgcagaag gttagatttt cgaaatcttt acaaaccagg
     3061 agtggtaaga gatatatcgg catttatcag atcgttaggt ggctataagt cacgctaaat
     3121 gactggctga atcgaatcat agccagagca tgccctgact tcaccccgct gtgtctgctt
     3181 ttcccgacta ttcttaatga gcttcgatgc aattcacgat cccgcagtgt gatttgagga
     3241 gttttcaatg gaatataaag atccaatgca tgagctgttg agcagcctgg aacagattgt
     3301 ttttaaagat gaaacgcaga aaattaccct gacgcacaga acaacgtcct gtaccgaaat
     3361 tgagcagtta cgaaaaggga caggattaaa aatcgatgat ttcgcccggg ttttgggcgt
     3421 atcagtcgcc atggtaaagg aatgggaatc cagacgcgtg aagccttcaa gtgccgaact
     3481 aaaattgatg cgtttgattc aagccaaccc ggcattaagt aagcagttga tggaatagac
     3541 ttttatccac tttattgctg tttacggtcc tgatgacagg accgttttcc aaccgattaa
     3601 tcataaatat gaaaaataat tgttgcatca cccgccaatg cgtggcttaa tgcacatcaa
     3661 cggtttgacg tacagaccat taaagcagtg tagtaaggca agtcccttca agagttatcg
     3721 ttgatacccc tcgtagtgca cattccttta acgcttcaaa atctgtaaag cacgccatat
     3781 cgccgaaagg cacacttaat tattaaaggt aatacactat gtccggtaaa atgactggta
     3841 tcgtaaaatg gttcaacgct gacaaaggct tcggcttcat cactcctgac gatggctcta
     3901 aagacgtgtt cgtacacttc tctgctatcc agaacgatgg ttacaaatct ctggacgaag
     3961 gtcagaaagt gtccttcacc atcgaaagcg gcgctaaagg cccggcagct ggtaacgtaa
     4021 ccagcctgta atctctgctt aaaagcacag aatctaagat ccctgccatt tggcggggat
     4081 ttttttattt gtttttcagg aaataaataa tcgatcgcgt aataaaatct agtattattt
     4141 ttgtgaaaaa taaatttggg tgcaatgaga atgcgcaacg ccgtaagaaa ggcgggaata
     4201 acttcccgcc gaagactctt actcttttaa attgcaggct aaaaatgccg ccagctcata
     4261 acacccctgt ttaatatgca attcacacag tgaatctctt atcatccagg tgaaaaataa
     4321 aagcgtgaaa caaatcacta ttaaagaaag taatctatat ttctgcggca ttcccagccc
     4381 cctgtgttga tttccaacga gtaagaggct aaccttatgg tgttcaggca tagggtagcc
     4441 tcactttgat ttatagtcag gtggggcttt tctctgtctg cctttcggtg aatacctgag
     4501 acaaacagtc tcaagcaccc gtggctattc tagcttaata agtttgtttc ttctccttga
     4561 tataatccta aaaaaacctc ataaaattaa tatatgagat aatctttatt cagcagaaga
     4621 ttattaaagg ttgctgtatt atttagcgat aataaaaaaa gcctgccaga tggcaggctt
     4681 tttaataacg gcgttattac tgcaacagcg aaatatccgc aacgcgcagg aacagttcgc
     4741 gcagtttctc cagcatggtc agacggttga gacgcaattc tttgtcatca accatgacca
     4801 tcactttatc gaagaaagca tcaaccggct cacgcagctc tgccagttcg accagcgcat
     4861 cctggtaacg accttccgca aagtacggct ccagcttgtc acgtagcacc acaacctgca
     4921 tcgccagttt aatttcttcc ggctctttca gggtagaggc attcacgcgg tcgctcagca
     4981 cttcgtcaga tttcgccaaa atgttggaga cacgcttgtt cgccgccgcc agtgcagcag
     5041 ctgcttcaag ggtacggaag tgcgataccg ctttcatgcg ggcatcgaaa tcagccggac
     5101 gagtcggacg acgcgccagt accgcctgga tggtgtcaac ggtgtaacct tcgtcctgat
     5161 accaggcgcg gaagcgaccg agcataaagt cgataacatc atcaaccacg ttggcgttag
     5221 tcagcttatc gccatacaga cgcactgctt cttcggtcag cgtttgcaga tcaaggttga
     5281 ggttcttctc aacgataatt cgcagcacgc caagtgcggc acgacgcagc gcaaacgggt
     5341 ctttgtcgcc tttcggatgc tggccgatac cgaagatacc cgccagagta tccatcttgt
     5401 cagcaatcgc cagcgcacag gctaccgggt tagacggcag gtcatcaccg gcaaagcgcg
     5461 gctgatactg ctcattcagc gccaccgcga catcttccgc ttcgccatcg tgacgcgcat
     5521 agtgcatccc cataacgccc tgggtgtcgg tgaactcgaa gaccatgttg gtcatcaggt
     5581 cacacttaga cagcaggccc gcacgggttg cgtggttaac gtcagcgcca atctgttcag
     5641 caatccagcc agccagcgcc tggatgcggt cagttttgtc gcgcagcgta cccagctgtt
     5701 gctggaacag cacggtttgc aggcgcggca ggttatcttc cagacgtttt ttacggtcgg
     5761 tattgaagaa gaactcggca tccgccagac gtggacgaac gactttctcg ttaccagaga
     5821 taatttgctg cggatctttc gattcgatgt tggcaacgaa gatgaagttc ggcagcagtt
     5881 tgccatcgtt cgcatacacc gggaagtatt tctggtcacc tttcatggtg taaaccaggg
     5941 cttcagccgg caccgcgagg aatttctctt cgaattttgc ggtcagaacg accggccact
     6001 ccaccagcga agccacttct tccagcaggc tttcgcttaa gtcagcgtta ccgccaatct
     6061 tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt acgttcttcg taatcggcga
     6121 tgactttccc gcgctcacgc agaatttccg gatactgatc ggcgttgtcg atggtgaatt
     6181 ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc ggactgaatg cccagaatgg
     6241 ttgccggaat gactttgtcg cccagcagca gggtcacggt gtgcaccgga cgcacgaagt
     6301 gcacgtcgct tgcgccccaa cgcatcagtt tcgggatcgg cagtttcgcc agagaagtcg
     6361 caaccatatt cggcagcagt gcttcggtgc tttcgccctt cacatgggcg cgatacagca
     6421 gccattcgcc tttatcggta gtcagacgct cagcctggtc aacggtaata ccgcaaccac
     6481 gcgcccaacc ttctgccgct ttgctcggtt tgccttcagc gtcgaacgcc tgggcaatcg
     6541 ccgggccgcg tttttcgatt tcacgatccg gttgcgcttc cgccaggtta gctactttca
     6601 gcgccagacg acgcggagca gcaaaccatt gaacggtgcc gtgtgcgagg ccagcgttat
     6661 ccagctccgc agtaaaattc gcagcaaagg actcagccag gctgcgcagt gcttttggtg
     6721 gcagctcttc agtgccgatt tccaccagaa aagttttctc agacatagcc gcctcttact
     6781 tatctttgtt gcacatcggg aagccgaggg cttcacggga agcgtagtat gcttctgcca
     6841 ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg acgctcggtg acggagatgg
     6901 ctttacgcgc atccagcagg ttgaagctgt gggcggcttt cagaatacgc tcgtaggctg
     6961 gcagcggcag cggattttcc agcgccagca gctgctgcgc ttctttctcg tactgctcaa
     7021 agcaggtgaa caggaagtcc acatccgcgt attcgaagtt gtaagtggac tgctccactt
     7081 cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag cgggccgtcg ctccagacca
     7141 ggtcgtaaac gctgtctacg ccctgaatgt acatggccag acgttccaga ccgtaggtga
     7201 tctcgccggt aaccggttta cactccagac caccaacctg ctggaagtaa gtgaactgcg
     7261 tcacttccat gccgtttagc cacacttccc agcccagtcc ccaggcaccc agagtcgggt
     7321 tttcccagtt atcttccacg aaacggatgt cgtgaatagt cgggtccatg cccagctctt
     7381 tcagagaacc gaggtacagc tcctgaatat tgtccggcga tggcttaatg accacctgga
     7441 actgatagta gtgctgtaaa cggttggggt tttcgccgta gcgaccatcg gtcgggcgac
     7501 gagaaggctg aacataagca gccgccatcg gttctggccc cagcgcgcgc agacaggtca
     7561 ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg ttgaacaatg gtgcagccct
     7621 ggcgagccca gtaatcctgt aaggtcagga tcaagccctg gaaggtcctg gtatcaaact
     7681 tttgcatatt atttcgtgct ggatacgtgt ggatttaaag gaagggatca gtatacccgc
     7741 tggatggaag atatacagta cgaaacggga aaaagcaggg cttaacgcat ggaaagatgc
     7801 aaaaaatggc catctgcgta aaaagaacag acaaactgct ctttacggaa ggtataaccg
     7861 cgcatttcat agcttccctg gaactgttcg aaggcggtga cgtcgatttt ttgctcgccg
     7921 gtgttataac gttttgccgc ctgatccttg cataacgctt ccatattcaa cgaacgcaga
     7981 ggatcaacct ttacccgttg tgctttctgc acgggtgacg gtgtagaaca acccactagc
     8041 accataagcg ccattgccgg aaagaaagcg ttcatcatca ttaaattacc gtctggtttg
     8101 caggtgagtc ttattattta tatcggtata gtggctgata tcttacgaat ttccttaagc
     8161 ctctataagc tgtagtgata aaactcagat tattccagtg cgccaaccaa atgatttatc
     8221 ggtcagactt tttttaacca gatacagaga cacagatgca gcccaaaatt tactggattg
     8281 ataacctgcg agggatagcg tgtttaatgg tggtgatgat tcacaccact acctggtatg
     8341 tgaccaatgc tcatagtgtt agccccgtca catgggatat cgccaatgtt ctgaattctg
     8401 cctctcgtgt cagcgtgccg ctatttttca tgatttccgg ctatctcttt tttggcgaac
     8461 gcagcgccca gccgcgccat ttcttgcgta tcggcttatg tctgattttt tatagcgcaa
     8521 tcgcactgct ctacattgcg ctgtttacct ccatcaatat ggagttagcg ctgaaaaacc
     8581 tgctgcaaaa gccagtgttt taccacttgt ggtttttctt cgcgattgcg gtgatttatc
     8641 tggtttcacc gctgattcag gtgaagaacg tcggcggaaa aatgttgctg gtgctaatgg
     8701 tggtgattgg cattatcgct aacccaaaca cagtgccgca gaaaattgac ggttttgaat
     8761 ggctgccaat taacttatat atcaatggcg atacttttta ctacattctg tatggcatgt
     8821 tgggccgcgc tatagggatg atggacacac agcataaagc actgtcgtgg gtgagcgccg
     8881 cgctgtttgc gacgggggtt tttattatct ctcgcgggac attatatgaa ttgcagtggc
     8941 gcggaaattt tgccgatacc tggtatcttt actgtgggcc gatggttttt atctgcgcaa
     9001 tcgcgctatt gactctggtt aaaaacacgc tggatacgcg taccattcgc ggacttggct
     9061 taatctcccg ccattcattg ggtatatacg gattccacgc cttgattatc catgcgctgc
     9121 gcacccgggg aattgagctt aaaaattggc caatactgga tattatttgg atcttttgcg
     9181 cgacgttggc agcgagtttg ttactttcta tgctggtaca acgaatcgac agaaacagat
     9241 tagtgagtta agtaaaagcc cgttcacatt ggactgaccg ggcttacgtg aattattcgc
     9301 tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt tattccggca tcacgaatat
     9361 tcttctgcac cgctactgca ccaaacaagc ttaagaagaa agccagtcca taaa
//
LOCUS       DAFATA010000142         9271 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFATA010000142 DAFATA010000000
VERSION     DAFATA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.143"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..329)
                     /locus_tag="LGJ20_004318"
     CDS             complement(<1..329)
                     /locus_tag="LGJ20_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBK2388312.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADVA
                     MEKSVELV"
     gene            complement(358..1212)
                     /gene="gatY"
                     /locus_tag="LGJ20_004319"
     CDS             complement(358..1212)
                     /gene="gatY"
                     /locus_tag="LGJ20_004319"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBK2388313.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(1522..2574)
                     /gene="fbaB"
                     /locus_tag="LGJ20_004320"
     CDS             complement(1522..2574)
                     /gene="fbaB"
                     /locus_tag="LGJ20_004320"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBK2388314.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            2831..4108
                     /locus_tag="LGJ20_004321"
     CDS             2831..4108
                     /locus_tag="LGJ20_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HBK2388315.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            4105..5109
                     /locus_tag="LGJ20_004322"
     CDS             4105..5109
                     /locus_tag="LGJ20_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBK2388316.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            5106..6071
                     /locus_tag="LGJ20_004323"
     CDS             5106..6071
                     /locus_tag="LGJ20_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK2388317.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(6045..6791)
                     /locus_tag="LGJ20_004324"
     CDS             complement(6045..6791)
                     /locus_tag="LGJ20_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2388318.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(6843..7661)
                     /locus_tag="LGJ20_004325"
     CDS             complement(6843..7661)
                     /locus_tag="LGJ20_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBK2388319.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYSYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(7726..8526)
                     /gene="thiD"
                     /locus_tag="LGJ20_004326"
     CDS             complement(7726..8526)
                     /gene="thiD"
                     /locus_tag="LGJ20_004326"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK2388320.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(8523..>9271)
                     /gene="thiM"
                     /locus_tag="LGJ20_004327"
     CDS             complement(8523..>9271)
                     /gene="thiM"
                     /locus_tag="LGJ20_004327"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBK2388321.1"
                     /translation="LHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEAS
                     QFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVAVGALDYRRRFCLEL
                     LSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEM
                     DYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVATCCALPGDTLENVASACHWMKQAG
                     ERAVARSEGPGSFVPHFLDALWQLTQEVQA"
BASE COUNT         2110 a         2427 c         2393 g         2341 t
ORIGIN      
        1 accagctcga cggatttttc catcgccaca tccgcatttt cttgctgcca gcagtttggc
       61 cccagatgat cgccgccgag aataatgcgt tcgcgtgcaa acccaacttt atcggcaatc
      121 gtaaaaacaa attcgcgaaa gtctgccggt gtcattccgg tataaccgcc aaattgattg
      181 acctggtttg acgttgcttc aatcagcact ttgcgcgtgc tgttgcgatc aaatgccagc
      241 gccgcttcga taaccaacgg atgggcagaa cagactgaac atatgccgat atgttcacca
      301 gctttatgcc gggcaattaa cgttttcatg ttttttcctt attaaatggc aggtgcgtta
      361 tgccctgccc tcgcagccac aatcggcaat cactttgctc accacatcgc gcattgcgga
      421 tttagccgac tgcaaataat cccggggatc ggtcgcttca gggtgctcgg tcaggtaatt
      481 ttttaacgcc tgcgagaagg catttttcag ctccgttgca acgttgattt tgcatatccc
      541 cagtttgatg gtttgctgaa tatccttagt cgataaccct gacgcgccat gcagcaccag
      601 cggtaagttc acccactggc gaatgttctc cagtcgagaa aaatcaagcg ccggtgcgct
      661 ggcatacatc ccatgagccg tgccgatggc gaccgccagg gaatcaattc cggttgcctc
      721 ggcaaattca cgcgcctgag cggggttggt ataaaaagca tcggcttcat tgacttgcac
      781 atcatcttcc tggccgccaa gttgccccag ctccgcttcg acgctgacat caaagcgatg
      841 gcaaaaatcc accacctctt tgacccgtga aatattttgc gcaaaaggca aatgcgaggc
      901 gtcaatcatg actgagcgca cgccagaacg aaccttctga gcgatatcgt caaatttcgt
      961 gtgatggtcg agatgaattg ccagtggatg gtgatattgc ttcgccatcg cgctgaccag
     1021 cgccaacaga ttttctgtac cagcatgagt aaatgtgcca ggcgttccgg cgatgatgac
     1081 cggcgcatgc aggttggcag cggtttccac caccacttgc atcgtttcga gattgtgaat
     1141 attgaatgcc ggaaccgcat aaccgccgcg ctgtgcgttg ttcagcatct gctttgtcga
     1201 taccacgtac attttcatat cctgtcgttt gttttcgatt tcaaaatata atgaaatgat
     1261 ttgtttttaa atgtcgagat aacgatcaca aaaacgacaa tgtgaaaatt gttcgaggag
     1321 taaaaggcaa aaaaaaacgg cctcccgata gggaagccgt agcaaagtgc gcgtgttttt
     1381 atgccggatg cggtgtaaac atacgtggca gtgctatgta ggcatgataa gacgcttccg
     1441 cgtcgcatca ggcataggtt gccggatgca gtgtaaacgc cttatccgtc ctacggggtg
     1501 gtgctgttcg tatgaggcgt atcaggcaat agtaattttg ctatcgagat aaacgtcctg
     1561 cacggcatta atcagtttca cgccgtcagc catcgatttc ttgaacgcct tacgtccaag
     1621 aatcagcccc attccgccag cgcgtttgtt gataaccgca gtacgcactg catcactgag
     1681 gtcagtttca ccgcctgcag caccgccgga gttaatcagc cctgcgcgcc ccatatagca
     1741 gttagctaac tgataacgca ccagatcaat cgggttttcg ctggttaact tgctgtacac
     1801 gcgatcgtcg gtataaccgt aattaattgc tttatagccg ccgttatttt ccgccatttt
     1861 ttgtttgacg atatctgcac cgatggttgc cgccagatgg tttgcctgac cggtcaggtc
     1921 ggcggaaaca tggtaatcaa cgccatcttt cttaaaggcg gagttacgca aataggccca
     1981 cagcactgtc accataccca gctcgtgcgc acgttcaaaa gccgcagaaa tttcttcaat
     2041 ctggcgacgt gactcttccg agccaaaata gatagtcgca ccaaccgcaa ccgcgcccat
     2101 gttgaacgcc tgctccacgc tggcatacag cgtttgatcg taggtattcg ggtaacttag
     2161 cgtctcgttg tgattaagtt tgacgaggaa tggaatgcga tgcgcataac gccgcgatac
     2221 cgacgccagc acgccgtaag ttgacgccac acagttacag cccgcttcga tcgccagttc
     2281 aacaatgttt ttcgggtcaa agtagagcgg gttagcagca aatgaagctc cggcagagtg
     2341 ctcaacgccc tggtcaaccg gcagaataga aagatatcct gtgccagcca gacgcccggt
     2401 gttgtacaac gtctgcatat tacgtaacac cgctggcggg cgattattgt caatcattac
     2461 gcggtctacg tagtcatgtc cggggagata aagctggtca gaaggaattg tcatacaacg
     2521 gtgctgtaaa aggttgtcgg cgtctttgcc aagcaactgc gcaatatctg tcattactat
     2581 gctcccgtaa attccgattg gatatcggct atggattgtc ctggcccgcc ttttgcgggc
     2641 aatcataatc ctggtcgtta cgggcgagtt tttccatctt ttggatgttt tttcagcgtt
     2701 ttctgttggc tcgattcatc agaaaaaatg ttagcgcggt caagttttca ccgcaaaggt
     2761 atttaaaagg tattattaag tggtattgtc atcgcgtacc ttacattacc tgtcatgaag
     2821 gaattaaaag atgaaaacaa cagcaaagct gtcgttcatg atgtttgttg aatggtttat
     2881 ctggggcgcg tggtttgtgc cattgtggtt gtggttaagt aaaagcggtt ttagtgccgg
     2941 agaaattggc tggtcgtatg cctgtaccgc cattgcggcg atcctgtcgc caattctggt
     3001 tggctccatc actgaccgct ttttctcggc gcaaaaagtg ctggcggtat tgatgttcgc
     3061 aggcgcgctg ctgatgtatt tcgctgcgca acagaccact tttgccgggt tctttccgtt
     3121 actgctggct tactctctaa cctatatgcc gaccattgcg ctgactaaca gcatcgcttt
     3181 tgccaacgtg ccggatgtgg agcgtgattt cccgcgcatt cgtgtgatgg gcactatcgg
     3241 ctggattgcc tccggtctgg catgtggttt cttgccgcaa atactggggt atgccgatat
     3301 ctcaccgact aacatcccgc tgctgattac cgccggaagt tctgctctgc tcggtgtgtt
     3361 tgcgtttttc ctgcccgaca cgccaccaaa aagcaccggc aaaatggata ttaaagtcat
     3421 gctcggcctg gatgcgctga tcctgctgcg cgataaaaac ttcctcgtct ttttcttctg
     3481 ttcattcctg tttgcgatgc cactggcgtt ctattacatc tttgccaacg gttatctgac
     3541 cgaagtcggc atgaaaaatg ccaccggctg gatgacgctc ggccagttct ctgaaatatt
     3601 ctttatgctg gcattaccgt ttttcactaa acgctttggt atcaaaaagg tattattgct
     3661 tggtctggtc accgctgcga tccgctatgg cttctttatt tacggtagtg cggatgaata
     3721 tttcacctac gcgttactgt tcctcggtat tttgcttcac ggcgtaagtt acgattttta
     3781 ctacgttacc gcttacatct atgtcgataa aaaagccccc gtgcatatgc gtaccgccgc
     3841 gcaggggctg atcacgctct gctgccaggg cttcggcagt ttgctcggct atcgtcttgg
     3901 cggtgtgatg atggaaaaga tgttcgctta tcaggaaccg gtaaacggac tgactttcaa
     3961 ctggtccggg atgtggactt ttggcgcggt gatgattgcc attatcgccg tgctgttcat
     4021 gatttttttc cgcgaatccg acaacgaaat tacggctatc aaggtcgatg atcgcgatat
     4081 tgcgttgaca caaggggaag ttaaatgaaa acagaacgta ttctcggtgc tctttatggg
     4141 caggcgttag gggatgcgat ggggatgccc tccgagcttt ggccacgcag ccgcgttaaa
     4201 gcacactttg gctggattga ccgttttctt cctggaccaa aggagaataa cgcggcctgt
     4261 tattttaacc gcgccgaatt caccgacgat acctcgatgg cgctgtgtct ggcggatgcg
     4321 ttactggaac gtgaaggcaa gatcgatccg gatctgattg ggcgtaatat tctcgactgg
     4381 gcgctgcgtt tcgacgcctt taacaaaaac gtactaggtc cgacctcgaa gattgcgctt
     4441 aacgccattc gcgacggtaa acccgttgct gaactggaaa acaacggcgt gaccaacggc
     4501 gcagcgatgc gcgtctcgcc attaggttgt ttgcttccgg cgcgtgatgt tgattccttt
     4561 attgatgatg tagcgctggc gtccagcccg acccataaat ccgatctggc ggttgcaggc
     4621 gcggtagtca tcgcatgggc gatttctcgt gccattgacg gagaaagctg gtcagcgatt
     4681 gttgattcac tgccttcaat tgcgcgacat gcccagcaaa aacgcaccac cacgttcagc
     4741 gcctcactgg cggcgcggct ggagattgcg ctgaaaattg tgcgcaatgc cgacggcacc
     4801 gaatccgcca gcgaacagct ttaccaggtc gttggcgcag gtaccagcac tattgagtcc
     4861 gttccgtgcg ccattgcgct ggttgaactg gcacaaaccg acccgaatcg ctgcgccgtc
     4921 ctgtgcgcta accttggcgg cgacacagac accatcggtg ctatggcgac ggcaatttgc
     4981 ggcgcgttgc atggcgttaa cgctatcgat cctgcgttaa aggcggaact ggatgcggta
     5041 aatcagcttg atttcaaccg ctatgccaca gcgttggcga aatatcgtca acaacgggag
     5101 gtggtatgag cggcgctcga ttacacacgc tgctgcctga attaaccacg cgtcagccgg
     5161 tgatggtcgt cggcgcggcg gtcattgacg tgatcgccga cgcttatgcc ctcccctggc
     5221 gtgggtgcga tatcgaactg aaacagcaga gcgttaacgt tggcggctgc gcactgaata
     5281 ttgccgtggc gttaaagcgc ctcggcatcg aagcgggtaa tgccttgccg ctcggtcagg
     5341 gcgtgtgggc ggagattatt cgcaaccgga tggcaaaaga gggcttaatc agcctgatcg
     5401 ataacgccga aggtgataac ggctggtgtc tggcgctggt tgagccggat ggcgaacgca
     5461 cttttatgtc attcagcggt gtggaaaatc agtggaatcg ccagtggctg gcgcgattaa
     5521 ccgttgcgcc tggcagcctg ctctattttt ccggttatca actggcctcg ccctgcggcg
     5581 aattgttagt ggaatggctg gaagagctgc aagacgtgac gccgtttatc gattttggcc
     5641 cacgtattgg cgatatcccg gatgcattac tggcgcggat catggcctgt cgacctttag
     5701 tgtcgctcaa tcgtcaagag gctgagattg ccgccgaacg ttttgcttta tccgcagaga
     5761 taacaacact tggcgagcaa tggcaggaga aatttgccgc gccgttgatc gttcgtctcg
     5821 ataaagaagg cgcatggtat ttcagcaacg acgcttctgg ctgcattccg gcatttccaa
     5881 cgcaagttgt agacaccatt ggggcgggcg acagtcatgc aggtggcgta cttgccgggc
     5941 tggcctctgg tctgccactg gcggatgccg tattactggg caatgcagtg gcgtcgtggg
     6001 ttgtcgggca tcggggcggt gattgtgcgc caacgcgcga ggaactactc ctcgcacaca
     6061 aaaacgtata gatcgctgcg acagtggcta atgctgtact caataggccg ttgctgttgg
     6121 tcaagcgcca cttgcttgat caccagcacc ggtattttgc tatctaactg aatatgtgac
     6181 tgaaactcgg catccggcat ccgggcgcta acgcgggaac gcgtacgttg tgggtaaatg
     6241 tgctggctgc ggaaatagtc ataaagtgaa atcccgatgg catcaacatc atgaattaaa
     6301 tgcgccggaa cccacgattc ctcaatcgat accgcttctt catcgacata gcgaatacgc
     6361 ttaagcaaaa agacatcact tcctgcttcg accgccagct gctgcgcgac ttcttcggga
     6421 catttcacga cacgcttgtt aacccatagc gtatcgggct ttttcccgcg caataccacc
     6481 tgctgagaaa aacctcgcgc ttctttcaac gagtattcga agatgttgtt gatctgcgtg
     6541 ccgtaaccgc gcgaacgcgt caccacaccc tcttcttcca gcgcctgcat cgccttgcgc
     6601 accgtaatgc gcgaaacgcc ggttaactga cttaaatcac gctcaccggg caaaatattg
     6661 ccatgctcca gcaccccgct gcgcacggca ttttttaccg tttcggcaaa cttaatataa
     6721 agcggcgtgt tatccgccgc taaaatacgc tcgttcagtt gggcaatcag ctgggtatgc
     6781 gcttgttcca tttgtctttt tcctgccgag gagtctggtg ggagtatatc actgcttctt
     6841 atttaaggcg tttctttaat cccatcaacg aatgcctgca actcctccac cgtgccatga
     6901 aatacattaa aatccaccgg accattgatg ccatctacct gtccacggtc ggaatgctgc
     6961 cagaagcgcc aggccatgcc gtcattgtcc ggacgacgtt gataatagtg agccacccac
     7021 cacggatatt cattgaaata gcccgccaga ttggtgtgat aaaaaacggc tcctgagtaa
     7081 ataatcggct tttttcccgt acttttttcg accattttta gccactgact tactcgtttg
     7141 cgtaattctt ttgccgataa ttttccccgt tcttctacgt ccagtaccgc agggaaatcg
     7201 ccttgtgaga aatccaccgt ttgcagaaat aatcgtgcct gaaccggcgc tgctaccgac
     7261 ggggagaaat aatgatacgc cccgcgcagc aggccatttt cgcggcttag ttgccagtta
     7321 cgcgaaaaat acgggtccac cagcttttcg ccttccgtcg ccttaataaa agcaaactgt
     7381 aagcggatac cgttgtcgcg catttttgcc acccgctgcc agtcgatccg ctcctgccag
     7441 cgcgaaacat ctattccgtg aatggtataa ctggcaggaa tgcgaattgc gaaagattta
     7501 accgggcgat aactgtagaa gtggatgtag tctgtaatct gaactgctgt cgaatagaaa
     7561 aaattcaccg tctgacgcgg ataaattgca acgatggaaa ttagcccaag agcgcataat
     7621 agagaggtga gtttttttcg actggtaatt cttagctgca ttggtttcat ccctgaatgt
     7681 cagtgccaaa ggctgacaat aaccaaagca gctatagtac ggtgctcacc accaggcgtg
     7741 gaagtggtga accggaccaa taccgtgacc aacttccagc gtgtcggcct gggctaatgc
     7801 tgatgaaagc cagctttttg cctcctgtac ggtgtcagcc cagtttgtat ggcgcgggcg
     7861 tagtgcagcc aacgccgcag agagcgtaca accagtgccg tgggtgtttt tggtcataat
     7921 gcgcggtgcg gtaaaccgtt gttcaccctc gcgggtaaac agccagtccg ggctttgctc
     7981 atcatccaga tgaccacctt tcattagcac tgcgccacag cccatcgcca acagcgatcg
     8041 cccttgttcc agcatttcct gttcggtgcg cgcgtgtggc gcgtcgagca aggcggcagc
     8101 ttcgggcaag tttggcgtta ttaatgaaac ctgtggcaat aatcgactgc gcagcgtagc
     8161 aaccgccgaa ggtgaaagca gcgggtcgcc gctttttgcc agcataacgg tgtcgagtac
     8221 cacgttttgg atctgataac gttgcaaccg ttctgccacc gcttcaacaa tatcggtttc
     8281 cgccaacata ccgattttag tggtgtcgat tcgcacatcg ctgaacaccg aatcgagctg
     8341 ggcggcgaca aaatcaggct caatgcgata caccgactgt acgccacggg tattttgcgc
     8401 caccagtgca gtaataactg agcaaccata agcgccaagt gccgagaagg ttttaagatc
     8461 ggcctgaatc cccgcaccac cactcggatc agtaccggca atcgtcagag cgttaattcg
     8521 tttcatgcct gcacctcctg cgtcaattgc cagagcgcat caaggaaatg tggaacaaaa
     8581 ctgcctggcc cctcgcttct ggcgactgcg cgctctccgg cttgtttcat ccagtgacag
     8641 gcagatgcga cattttccag cgtatcgcct ggtaacgcac agcaggtagc gacaaccgcc
     8701 gataatgcac agccagttcc taccactttg gtcattaacg gatcgccacc gtgaatacca
     8761 atgatacgat gtccatcggt aacataatcc atctcgccag tgaccacgac gattgcgcca
     8821 gtttcccgtg ccagtgtttg tgcagcgggt atcgcgttag ctgcggcgtc agtggtatcc
     8881 actccccgtc caccattagc aatgccagct aatgccatga tttccgaagc attaccacgt
     8941 atggcggttg gcttatgaga cagaagttcc agacaaaaac ggcggcgata atcgagtgca
     9001 ccaaccgcta ctggatcaag cgtccagggt gtttgagagc tttttgcttg ctcaacggcg
     9061 gcacacatcg actgtgcgcg tagctgcgtc agcgtgccaa cgttaatcag caacgcactg
     9121 gcaatagccg caaactgact ggcctcttcg gtttcgataa ccatcgctgg cgatgcaccg
     9181 agcgccagca aggtattggc agtaaaggtt tgcaccacat cgttggtcat gcagtgcaca
     9241 agaggggaat gttggtgaaa aaggtgtaac g
//
LOCUS       DAFATA010000143         9017 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFATA010000143 DAFATA010000000
VERSION     DAFATA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.144"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(115..822)
                     /locus_tag="LGJ20_004328"
     CDS             complement(115..822)
                     /locus_tag="LGJ20_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK2388322.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1220..3355
                     /gene="speC"
                     /locus_tag="LGJ20_004329"
     CDS             1220..3355
                     /gene="speC"
                     /locus_tag="LGJ20_004329"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBK2388323.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3405..4661)
                     /gene="nupG"
                     /locus_tag="LGJ20_004330"
     CDS             complement(3405..4661)
                     /gene="nupG"
                     /locus_tag="LGJ20_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBK2388324.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4863..5942)
                     /gene="mltC"
                     /locus_tag="LGJ20_004331"
     CDS             complement(4863..5942)
                     /gene="mltC"
                     /locus_tag="LGJ20_004331"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBK2388325.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6007..6282)
                     /locus_tag="LGJ20_004332"
     CDS             complement(6007..6282)
                     /locus_tag="LGJ20_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBK2388326.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6310..7362)
                     /gene="mutY"
                     /locus_tag="LGJ20_004333"
     CDS             complement(6310..7362)
                     /gene="mutY"
                     /locus_tag="LGJ20_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK2388327.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7523..8242
                     /gene="trmB"
                     /locus_tag="LGJ20_004334"
     CDS             7523..8242
                     /gene="trmB"
                     /locus_tag="LGJ20_004334"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK2388328.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8242..8568
                     /locus_tag="LGJ20_004335"
     CDS             8242..8568
                     /locus_tag="LGJ20_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBK2388329.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8752..>9017
                     /locus_tag="LGJ20_004336"
     CDS             8752..>9017
                     /locus_tag="LGJ20_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 family protein"
                     /protein_id="HBK2388330.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWID"
BASE COUNT         2184 a         2234 c         2406 g         2193 t
ORIGIN      
        1 tatccgggca acggggcgca ttaaacctga ttcgcctcgt ctcgtcaatc aaatttcttc
       61 aatttactgc agactgcaca atctatcatc actttgccgc tattgcacgc attgtcaggc
      121 aaaccaggcg gtccaggcgg cggaaagcgg cattgccaga aggagtgctg gaagcatgtt
      181 gaccaccggg aacattttaa tgccacagat gcgtaatccg gttgccagca gcaataaacc
      241 gcctacggcg ctgaagtctg ccatcatcga cggtgtggtc agcggtaata tcagcgcggc
      301 agcccacgcc agcgttaact ggatgatcag taatgggata ctaattaccg acaccgcaat
      361 acccagcgag caggcgaaga tcatcgccgt aaagaaatca agaaatgact tggcgattaa
      421 aatactcgga tcgccggtca tcccttcgtt catcgccccg aagatcccgg tgccgctggc
      481 gcaaaacagg acaataatcg cgacataatt ctgaataaaa gattcatgcg ctggcttctt
      541 acgtgagtga cgaaacagat tttgtgcttt ggcgactgct gtattgacac ctttctccag
      601 caaacaaatt tcgccaatta gagcgccaag tagcgtagct aaaaccatcg ctggaaggtt
      661 ggcgcatttc acaaccagta aaataccaat ccccagcgat gccagaccaa aaatagatgt
      721 catggagacg cggatacgtt ccggtaagcg ttggctgagc agtgcgccga gaacgccacc
      781 cagtaagact gcactagcgt tgataaaagg gccgatgacc acaagagttc ctgttagctg
      841 tattgtttga ttttcttatt atgaactttt tgccttgttg atggcttgtc tggtcgcaaa
      901 gtcctggctt gcacgcttta gcgaaaggtg ccatgattgc gcgaattttc tcctctctgt
      961 acggagtttg cccgatgcac gccatctcct tacattctct cgcttatcgc cgtttcgcgc
     1021 gaaactcttc tctttttatg ctactgctca tgcggtgaag ttaacgcacg ttcactgcag
     1081 gacaacagta aaatcagagc gtttctgctt ttactgatgt ctggcggtcg gagctggtga
     1141 ccagtttgac ccatatctca tggggcaggg ttttccacct tgtccggtat tcttacttcc
     1201 ccgaaacggg tttgcgctta tgaaatcaat gaatattgcc gccagtagtg aactggtatc
     1261 ccgactttct actcatcgcc gcgtggtggc gttgggagat actgatttta cggacgtcgc
     1321 ggcagtcgtc attaccgctg cggatagtcg cagtggcatt cttacgttgc ttaagcgcac
     1381 cggttttcat ctaccggtgt ttttgtattc cgaacatgct gttgaattac ctgcgggcgt
     1441 tacggcggta atcaacggca acgagcagca ttggctggag ctggaatccg cagcctgtca
     1501 gtatgaagag aatttgctgc caccgtttta tgacacgctg acgcagtacg ttgagatggg
     1561 caacagcacc tttgcttgcc ctgggcatca acatggtgcg ttttttaaaa agcatcctgc
     1621 cggacgccat ttttacgatt tctttggtga gaacgtcttt cgcgccgata tgtgtaatgc
     1681 tgacgtaaaa ttgggcgatc tgcttattca tgaaggatcg gcgaaagatg cgcagaaatt
     1741 cgcagccaaa gtctttcatg ccgataaaac ctattttgtg ctgaacggca catcggcagc
     1801 gaataaagtg gtgacgaatg cgctgttaac gcgtggcgat ctggtgctct tcgaccgtaa
     1861 caaccataag tcgaatcatc acggcgcgct gattcaggcg ggggcgacgc cggtctatct
     1921 ggaagcttca cgcaacccgt ttggtttcat tggcggtatt gatgcgcact gttttaatga
     1981 agagtatctg cgccagcaaa ttcgcgacgt tgcgccagaa aaagccgacc tgccgcgccc
     2041 gtttcgcctg gcgattattc agctgggaac ctatgacggc actgtctata acgcccgtca
     2101 ggtgatcgat accgttgggc atctgtgtga ttacattctg tttgattccg cgtgggtcgg
     2161 ttatgaacaa tttatcccga tgatggcgga tagctcgccg ctgctgttag aacttaacga
     2221 aaacgatccg gggatttttg tgacccagtc ggtgcacaaa cagcaggctg gattctcaca
     2281 gacgtcgcag atccataaaa aagataacca tatccgcgga caggcgcgtt tttgcccgca
     2341 taagcggttg aataacgcct ttatgctcca tgcttctacc agcccgttct acccgctgtt
     2401 tgccgcgctg gacgttaacg ccaaaattca tgaaggggag agtgggcgtc ggctgtgggc
     2461 tgagtgcgtt gagttgggta ttgagtcgcg caaggctatt cttgcgcgct gtaagctgtt
     2521 ccgcccgttt atcccccccg ttgttgatgg caaattgtgg caggattatc cgacatcagt
     2581 gttagccagc gaccgccgtt ttttcagttt tgagccgggg gcgaagtggc acggctttga
     2641 aggatatgcc gcggatcagt attttgttga tccgtgcaag ctgttactca ctacaccagg
     2701 tatcgatgcc gaaaccggcg aatatagcga ctttggcgtt ccggcgacga ttctggcgca
     2761 ctatctgcgt gagaacggca ttgtgccgga gaagtgcgat ctcaactcca ttctgttctt
     2821 attaactccg gcggaaagcc acgagaagct ggcacaactg gtggcgatgc tggcgcaatt
     2881 tgaacagcat attgaggatg actcgccgct ggctgaggtg ttgccgagcg tttataacaa
     2941 atatccggtg cgctatcgcg actacaccct gcgccagttg tgtcaggaga tgcacgatct
     3001 gtatgtcagt ttcgacgtca aagacctaca aaaagcgatg ttccgccagc agagtttccc
     3061 gtcagtggtg atgaaccccc aggatgcgca tagcgcttat attcgcggtg acgtggagtt
     3121 ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa ggggcgttgc cttatccacc
     3181 tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg gcggttcaac gttatttcct
     3241 tgcactggaa gaaggggtga atttgttgcc gggattttcg ccggagctgc aaggtgttta
     3301 tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt tatgtgttga agtaagaata
     3361 aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc gtaattagtg gctaaccgtc
     3421 tgtgtacctg tcgggacacg aacgtgttta tatttgaaca tcgccatgaa cgcgaaggcc
     3481 agaaccacgg agtaaccagc gaaaatcaac catacggtct gccagtcggt aatgccgttt
     3541 tgggtgtaca tctcaaccac tttaccgctc acgatgccac cgaggataca gccgaagccg
     3601 ttggtcatca tcaggaacat cccttgcgca ctggcgcgaa tcgccgggct aacttctttt
     3661 tcgacaaaca ccgaaccaga gatgttgaag aagtcgaacg cacagccgta aacaatcatc
     3721 gacagaacca gcagtacggt gccgaacgga gtcgggtcgc cgtaagcaaa cagcgcaaaa
     3781 cgcaggatcc acgccacgat actgatcatc attacgttct taatgccgta gcggcttaag
     3841 aagaacggga tggtcagaat gaacagggtt tcagagatct gcgaaatcga catgatgatt
     3901 gacgcatgct gcacaataaa gctgctggca aacatcggat ctttgtcgaa gctgtgcagg
     3961 aaggtattac cgaacatgtt ggtaatctgc agttccgcgc ccagcagcat tgagaagatg
     4021 aagaagattg ccatacgctt gtttttaaac agcgcgaatg catcgaggcc cagcagggtt
     4081 gtccagctct gattcgcttg ctgtttagca accggaatat gcggcagagt cagggtaaac
     4141 agaaccagaa tggcggaaag agctgcgcca atatacagct gcatgtggct taattcgaag
     4201 ccagacaggc tcaccaccca cattgccatg ataaagccga tggtgcccca gatacggatt
     4261 ggtgggaagt cagtaacgat atccatcccg gcattttgca ggcgatagta agagatggtg
     4321 ttgattaacc caagcgttgg catataagca aacgagttaa tcaatatcac aaggaacatc
     4381 gcctccggcg tcgtgacctg tgctgccatg aacagcgtga tagcgccaat ggtgtggcaa
     4441 atggcatata cccatttcgc acttaaccat ttgtcggcca caatccccag cagcgcaggc
     4501 ataaagaccg cggcgatacc cagtgagcta taaactgcgc caatagaagc accgtcaaac
     4561 ttcagagtaa caaacatata ggagccgagg gtcgtcagcc aacttcccca cagacagaac
     4621 tgcagaaaag agaggatttt cagctgcagc ttaagattca tgttaatttc ctcacatcgt
     4681 gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg agatgatggg attctatcaa
     4741 cttcgcggac tggttttttg ttacctgtgg caaataattg caaacattcc caatttgcaa
     4801 aacgaaattg tgatgtggat aacatttttg cccctgagca tcgtcagggg cggttaatgg
     4861 aattatcggc ggcggtagga tttttgcgcg gtattcactt tataaagata acggcgagat
     4921 tccgcagagg gatggcgggt cgtcagcgtc tgataaacat cgcccggcgt catggtgtta
     4981 ataatattgg cagcctgaat cttatcattc gaaaagactc gcagcacgct gcctgcgccg
     5041 ccgttatagg cggtgatgac ggcataacgc cgcgatgttg ggttatcaat tccgccgaga
     5101 taaacattgt tcagcatcgc cagatacgcg gtgccggtat caatattgct ggcaggatca
     5161 aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg agcggaacac atctttcccg
     5221 gcagtatgtt gtaccacctg cattaatccc agcgcatcgg aacggctgac cgcatacggg
     5281 ttaaaggaag attcggtctg cataattgcc agaatcagcg actcatcaac gccatatttc
     5341 cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac gtttatcaag gtggttcggt
     5401 accatgttaa tggtgacgct gtagatgata cgcagcccgt tactgcggct ttgcagacgg
     5461 tttttcagca gataatccgc gaagttgctt gcgcgacctt cccagcgaat cggctgcccg
     5521 gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg aaatcgtaat atcatcaacg
     5581 tcggaataga gatcgaccga actcggatcg tcacccatca ataacgtttt gataattgcc
     5641 cggcgcaaat gcgcggcagg ttctgtcccg gcgatggttt cgatagtaat cgtaccgtca
     5701 tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt atttcacgta gtccttagga
     5761 ccagcgatca ccacctcttt gaatccccag atgttctcaa tattgtgggc aaattgcccc
     5821 atcagaatat caaaaccgtt ggtgtctttg acccaggctt cgttataggt atcgcctttt
     5881 ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag ccagcgcgag atattttttc
     5941 atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt gccggaggcg tgctccggca
     6001 ctgtttttat tttttatctt ccggcgtata gccctcgata tgcacctctt taccctcgaa
     6061 caggaagttg accatctcct gctccagcag cttgcggtgc tcggcattca tcatgttgag
     6121 tttcttttca ttaatcagca tggtttgttt gtgctgccac tgcgcccagg cttctttgga
     6181 gatctcgtta tagatgcgtt ttcccagctc gccggggtac agctgaaaat cctgaccttc
     6241 tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc ataaatcatc ctctttatcg
     6301 gctcacgcgc taaaccggcg cgccagtgcg taactgctgt aacaaacgct ccacgggagc
     6361 cgccaggcca actgacggcg gttgcgctaa gttataccag agcgcattgc cttcatccat
     6421 gcagccggtg aatgacgaca cgggaagcca cataggcaca atatctaagt ggaaatggct
     6481 gaaggtatgg cgaaacgcgg tcagctgcgt caggttatcg gcagcaatct gccgttgcgc
     6541 cagccaatgc cgcaaacttt cttcgtcggc aaactgcggg aaacagtata aaccgcccca
     6601 caatccgctc ggcggacgct gcgccagcaa tacttcatct tcgtgctgta atagcaaaaa
     6661 gtagccggtg cgctccggca gcgtctgttt cggttttttg cccggataaa gcgcccagct
     6721 attgttggcg gcggcaatac atccgttttg tagcggacag agcgaacatt tcggtttcga
     6781 gcgcgtacaa atcatcgcac ccaaatccat catcgcctga ttaaaccgtt ccacgccgac
     6841 cgcgggcgtc acctgctcgc ttaaactcca taatttattc tcgacctctt ttttcccagg
     6901 ccagccgctt acagcatagc agcgcgccag cacgcgtttg acgttaccgt cgagaatcgg
     6961 aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg gtggaacgcc cgacgcccgg
     7021 cagtgctgca acttcctcaa aggtttccgg gaatttaccg ccgtgtaagg tcgccacttg
     7081 ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag ccaagcccgg tccacaagtg
     7141 gagaacttcg tcgagcggcg cattggcgag atcggtcacc gtcgggaagc gcgccataaa
     7201 gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt tgcaacatca cttctgagag
     7261 ccatactttg tagggcgtct tgtcaatttg ccagggcaga gtttttcgcc cgtatttatc
     7321 gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc atggtcaccg aattcactgt
     7381 tgttgggggc aagattgcag cacagcgacg gcagggtgta aaccggaact ttccgcagcc
     7441 acggccttat catgcttgca tccggcaact aactttggat aatgcccgtt ttcagaacac
     7501 tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt tcaccggaat ttgatgaaaa
     7561 cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc caggggcgac tgaccaaagg
     7621 ccaggaacat gcgctggaaa actactggcc ggtgatgggc gttgagttca gcgaagatat
     7681 gctggatttc cccgcgcttt ttggccgtga agcgccggtg acgcttgaga ttggttttgg
     7741 catgggggcg tcgctggtgg caatggctaa agatcgcccc gagcaggact tcctcggcat
     7801 tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct gcgcatgaag agggcttaag
     7861 caacctgcgc gtgatgtgtc acgatgcggt tgaagtgctg cataaaatga ttcctgacaa
     7921 ttcattgcgc atggtgcagc tctttttccc tgacccgtgg cacaaagcgc gccataataa
     7981 acgccgtatc gttcaggtgc cgtttgccga actggtaaaa agcaaactgc agctgggggg
     8041 cgtattccat atggcgaccg actgggaacc ttatgcggaa catatgcttg aagtgatgtc
     8101 ttctattgac ggttataaaa acctgtcaga gagcaatgat tacgtaccgc gtccggcatc
     8161 acgtccagtg acgaaatttg aacaacgtgg tcatcgtctt ggtcacggag tatgggactt
     8221 aatgttcgag agggtgaaat aatggcaaag aaccgtagcc gtcgtctgcg taaaaaaatg
     8281 cacatcgacg aattccagga attaggattt tcggtggcat ggcgattccc ggaaggtaca
     8341 tcggaagaac agattgataa aaccgttgat gattttatta acgaggttat cgaaccgaac
     8401 aaactggcct ttgacggcag cggttatctg gcctgggaag gtctgatctg catgcaggaa
     8461 atcggcaaat gcaccgaaga acatcaggca attgtgcgta agtggctgga agagcgcaaa
     8521 ctggatgagg tacgcaccag cgaacttttc gacgtttggt gggactaaga aagcatacgg
     8581 gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat catgcctgga aagatgcacg
     8641 atcgagtagg ccggataagg cgtttacgcc gcatccggca tggaaaacgc gtactttgtt
     8701 atcaatctgg ggccagcaaa tgctggcctg atttgttctt gagggaagac tatgatgcgc
     8761 aaaatgctgc tggcggcagc actttcagtg acggcaatga ccgctcacgc cgactaccag
     8821 tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc aaaccgtgca ggtgaagggc
     8881 gaaaacggca atctggtgat cacgccagac ggcaacgtga tgtataacgg taagcaatat
     8941 tccctgaatg ccgcccagcg cgagcaggcg aaggattatc aggctgaact acgcagcacg
     9001 ctgccgtgga ttgatga
//
LOCUS       DAFATA010000144         8972 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFATA010000144 DAFATA010000000
VERSION     DAFATA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.145"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            31..1188
                     /locus_tag="LGJ20_004337"
     CDS             31..1188
                     /locus_tag="LGJ20_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBK2388331.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(1154..1276)
                     /locus_tag="LGJ20_004338"
     CDS             complement(1154..1276)
                     /locus_tag="LGJ20_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388332.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            1479..3164
                     /locus_tag="LGJ20_004339"
     CDS             1479..3164
                     /locus_tag="LGJ20_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBK2388333.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPAGSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            3202..5574
                     /locus_tag="LGJ20_004340"
     CDS             3202..5574
                     /locus_tag="LGJ20_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBK2388334.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDDNNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYDGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5631..8414
                     /locus_tag="LGJ20_004341"
     CDS             5631..8414
                     /locus_tag="LGJ20_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK2388335.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNIQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            8776..>8972
                     /locus_tag="LGJ20_004342"
     CDS             8776..>8972
                     /locus_tag="LGJ20_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBK2388336.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQT"
BASE COUNT         2619 a         1954 c         1970 g         2429 t
ORIGIN      
        1 ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc ctccagtttt
       61 caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca gtttactcat
      121 gaaaaatgga tggatgacag tactctgaaa gagttcatca aacaatatat cgcagcgtct
      181 ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt
      241 ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat ttttaatgca
      301 ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa agaacatgaa
      361 tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta cagacgcagt
      421 aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat
      481 caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta ccctcttgag
      541 gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctagaa
      601 accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat
      661 ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt
      721 aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag ccgttttttg
      781 gggaatgggc atacaagttg tattttccag gagtcctgca aggataatct ggttgttgaa
      841 agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac
      901 attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg
      961 atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg ttgtcctaag
     1021 catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg ttataaaatc
     1081 cttttttcaa ccatggtacc ttatatgaac gccatggtgg agttagctaa gaacagagta
     1141 ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata acgctctctt
     1201 ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca taaattcctg
     1261 tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt ggtatgcgtc
     1321 agggcattag caataccctt gattatcaat agcataacca taaccgatga aaatgaaata
     1381 tttctttata tttcatattg ttaagtgata cttaaataaa tttcatcagt aaatcattgc
     1441 catagataat cattggatgg cacaatcaat tttcgctaat gataaccatt cccattacgt
     1501 tttgtatgtt gatagcaaag tatctctgtc tgctgaagcc cttttggctg cgtaagaata
     1561 ataaaacttc agtgttgtta atcattatta tcctcgcaat gatccttggc gtggttaaaa
     1621 ttcaggtctg gttaaacgac tggaataatg attttttcaa tgcgctaagc cagaaagaaa
     1681 ccgataagct ttggcagctt gtcctttggt tccctgccct gctggggatc tttgtattaa
     1741 tatccgtaaa caaaacatgg ttaattaaac tactgactat tcgctggcgc gaatggttaa
     1801 cagattacta ccttaatcgc tggtttgcag ataaaaacta ctacttcacg cagatctacg
     1861 gcgagcataa aaatacagat aaccctgacc aacgtatcgc tgaagacatt ctcttactga
     1921 tcagcaaaac gctcagtctt tcatttggct tcatccagtc acttagcatg ctgatcacct
     1981 ttaccgttat tctttggcaa agcgcgggta cgctctcatt cactgttggt ggaacagaat
     2041 ggaatatcca ggggtatatg gtctataccg tcgtgctcat tgtgatcggt ggaactttat
     2101 ttacccataa ggtgggtaaa cgtattcgtc cgcttaatgt ggaaaaacaa cgtagcgaag
     2161 cgacatttcg aactaatctt gtgcagcata ataagcaggc agaactgatt gcgctaagca
     2221 atgctgagtc tctacaacgc caggagttgc gcgacaattt tcataccatt aaagagaact
     2281 ggcatcgttt aatgaatcgt cagcggtggc ttgattactg gcaaaatatt tattcgcgct
     2341 cgcttagcgt tcttccctac tttctgttat tgccgcagtt tattagcggg cagatcaatc
     2401 tgggcggact gatgaaatcg cgccaggcat ttatgctagt atcgaacaat ttaagctggt
     2461 ttatttataa atatgacgaa cttgctgaac tggctgcggt tatcgatcgc ttgtatgagt
     2521 tccatcaact cactgaacag cgccctacga ataagcctaa aaattgccaa catgcggtac
     2581 aagtggctaa tgcgagtatt cgtacgcctg ataataagat catattagag aacttgaact
     2641 ttcatgtttc gccaggcaaa tggctattac tgaaaggcta ctctggtgcg ggaaaaacca
     2701 cactgcttaa aacattatcc cactgctggc cgtggtttaa aggtgatatt tcttctcctg
     2761 ctggcagttg gtatgtgtca caaacaccgt taatcaaaag cggcttactg aaagagatta
     2821 tttgtaaagc acttcccctg cccgtagacg ataaatcgtt gagcgaagta ctgcatcagg
     2881 ttggtctggg gaaattggct gcgcgtattc atgaccacga tcgctgggga gatattcttt
     2941 ccagcggcga aaaacaacgt atcgccctgg cacgattaat tttacgacgt ccgaaatgga
     3001 tatttcttga cgaaactacc tctcatcttg aggaacaaga ggctatccgc ttactgcgtt
     3061 tagtgcgtga aaaactaccc acaagcggcg tcattatggt tacacatcaa cccggtgtct
     3121 ggaacctggc cgatgatatt tgtgacatta gcgcggtttt ataaaaacaa taaaagccct
     3181 gctgtacgga gatataaata aatgaagcga gttcttattc ctggcgtcat tttatgtggc
     3241 gctgatgtgg cgcaggccgt cgatgacaaa aacatgtaca tgcatgtttt tgaagagatg
     3301 acggtctatg ctcctgtccc tgtacccgta aacggcaaca cgcattacac cagtgaaagc
     3361 atcgagcgtt taccgaccgg gaatggcaat atcagcgatc tgctgagaac caaccctgcg
     3421 gtacgcatgg attcaacgca aagtacctcg ttgaaccagg gagatattcg cccggagaaa
     3481 atctctattc acggtgcgtc gccctaccag aatgcctatt tgattgatgg tattagtgcc
     3541 actaataacc tgaacccagc gaatgagtcc gatgccagta gtgcaaccaa tattagcggg
     3601 atgtcacagg ggtattatct tgatgtcagc ttactggaca atgtgacgct ttatgacagt
     3661 tttgtgccgg ttgaattcgg tcgcttcaat ggcggggtaa ttgatgcaaa gatcaaacgc
     3721 ttcaacgctg atgatagcag cgtgaaactg ggttatcgca ctacgcgttc ggactggtta
     3781 acatcgcata tcgatgacaa taacaagagc gcatttaatc aaggttcttc aggaagtact
     3841 tattactctc cagattttaa aaagaacttt tataccttgt cgtttaatca ggaactcgct
     3901 gataactttg gcgttaccgc cggtttatcg cgccgccagt ctgatatcac ccgcgcggat
     3961 tatgtttcga atgacggcat tgtcgccggt cgggcacagt ataaaaacgt tatcgatact
     4021 gcattgagca aatttacctg gtttgccagc gaccgcttta cccacgattt aaccttaaaa
     4081 tataccggct ccagccgtga ttataatacc agcaccttcc cgcagtctga tcgcgaaatg
     4141 ggtaataaat cctatggtct ggcatgggat atggatacgc agctcgcatg ggccaaactt
     4201 cgtaccaccg ttggttggga tcatattagt gattataccc gtcacgatca tgacacctgg
     4261 tacaccgaac tttcatgtac atatggtgat attacagggc gttgcacccg tggcggatta
     4321 ggacacattt cccaggctgt agataattac accttcaaaa cacgcctgga ctggcaaaaa
     4381 ttcgccgtgg gtaatgtttc gcatcaaccc tacttcggcg cggaatacat ctattccgat
     4441 gcatggactg aacgccataa ccagtctgaa tcctatgtga ttaatgctgc cggaaagaaa
     4501 actaaccata ccatttacca taaaggtaaa ggcagcctgg gaattgacaa ctacacactg
     4561 tatatggcgg atcgcattag ctggcgtaat gtgtcattaa tgcccggcgt gcggtatgac
     4621 tatgacaact atctgtcaaa ccacaatatc tccccgcgct ttatgacgga atgggatatt
     4681 tttgctgatc aaacctcaat gattaccgca ggttataacc gttactatgg tgggaatatt
     4741 cttgatatgg gattacgtga tatccgcaat agctggacgg aatcggtatc aggcaataaa
     4801 accctgacgc gttatcagga tttgaaaacg ccttataacg atgaactggc aatgggattg
     4861 cagcagaaaa tcggtaagaa cgttattgcg cgtgcaaact atgtttaccg tgaagcgcat
     4921 gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat
     4981 aacaacgatg gcaaaaccaa aacgcattcg ttcaacctca gttttgagct ggccgaaccc
     5041 ctgcatatcc gccaggtaga tattaaccca caaattgtct ttagctatat caagagcaag
     5101 ggcaacttgt cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt
     5161 tatgacggta atctggtttc ttacgatagc gttccagtgg cagattttaa taacccatta
     5221 aagatctcct taaacatgga tttcacgcat caaccgagcg ggttggtgtg ggcgaatacg
     5281 ctggcctggc aagaagcgcg taaagctcgc attatcctgg gtaagacaaa tgcgcaatac
     5341 atcagcgaat attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc
     5401 tgggacaccc gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt
     5461 gccgatattc tcaatgtatt ggatagcaaa accgctgttg atacaacgaa caccggtgtg
     5521 gcgacctacg ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc
     5581 ggattttact taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc
     5641 tctgtttctt actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg
     5701 ccttaccgca ggatgaaaag ttaattaccg ggcaactggg caatggcttg cgatatatga
     5761 tttatccgca tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt
     5821 cattgcagga agaagacaat gagcgcggcg tggctcattt tgtagaacat atgatgttta
     5881 acggcacaaa aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc
     5941 gttttggtcg cgatgttaat gcctatacca gctatgacga aacggtgtat caggtgagtt
     6001 tgccgactac gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta
     6061 atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat
     6121 ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc gcgccgtcct ttcctgctgg
     6181 ctaatacccg taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca
     6241 caccggcaca attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta
     6301 tcgtggtcgg cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta
     6361 agcttccggc taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc
     6421 tgcgctttaa tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc
     6481 gcctgccaat ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca
     6541 tgttagttca gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga
     6601 ctatttctgg cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt
     6661 tccgtgtaaa tgcacgagac gataatatac aggatgctgc gaatgcatta atggcagagt
     6721 tggcaaccat tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc
     6781 gcctcacctg gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga
     6841 ccagtcgcct ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat
     6901 atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc
     6961 agcagttaag aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg
     7021 ccaaaaaagc attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa
     7081 agctggcggc ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac
     7141 aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca
     7201 atggtgccag ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattactg
     7261 ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg
     7321 caaataaagc agtaagcgga agcggcgttg gtgagctctc ctcttccagc ttgaaacgct
     7381 ggagtgcgga aaattcggta accatgagca gtaaagtcag tggcatgaat acgttgctct
     7441 ccgttagcgc gcggactaat aaccctgaac ctggttttca gttgattaac cagcgaatca
     7501 cccacagcac gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt
     7561 tgaaaacgct cgaccagcgt ccagcggaga aattcgccca gcagatgtat gagacgcgct
     7621 atgctgatga ccgcacgaaa ttactgcaag aaaatcagat tgtacagttt actgccgcag
     7681 atgcgctggc tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg
     7741 tcggtaatgt ctcagaagac aaactcgtgg cgttaattac gcgttactta ggatcaatca
     7801 aacactctga ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat
     7861 cggttactgt aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg
     7921 attcccggac acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac
     7981 tggcaaaaga tctacgtgtt aatattcgtg aacaggcatc tggagcatac agcgtttctt
     8041 ctcgcctctc ggttgatcct caggccaaag atatcagtca tttgctggct tttacttgtc
     8101 aaccagaacg acatgatgaa ctgttaacgt tagcgaatga agtgatggtt aagcgtctgg
     8161 ctaaagggat cagtgagcaa gaactgaatg aataccagca aaacgttcag cgcagcctcg
     8221 atatccaaca gcgtagcgtt caacaattag cgaacactat tgtaaatagt cttattcaat
     8281 atgacgatcc tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga
     8341 atgttaacac tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat
     8401 tattgccaaa ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc
     8461 gctattttta tgtaataatt ttataaatgc gttcaaaata ataatcaagt actaatagtg
     8521 atattttaag gtctgatttt tacgtgataa ttcaggagac acagaatgcg cataaaaata
     8581 acagcataaa acaccttacc accacccaag aatttcatat tgtattgttt ttcaatgaaa
     8641 aaatattatt cgcgtaatat ctcacgataa ataacattag gattttgtta tttaaacacg
     8701 agtcctttgc acttgcttac tttatcgata aatcctactt ttttaatgcg atccaatcat
     8761 tttaaggagt ttaaaatgga taagaagcaa gtaacggatt taaggtcgga actactcgat
     8821 tcacgttttg gtgcgaagtc tatttccact atcgcagaat caaaacgttt tccgctgcac
     8881 gaaatgcgcg acgatgtcgc attccagatt atcaatgacg aattatatct tgatggcaac
     8941 gctcgtcaga acctggccac tttctgccag ac
//
LOCUS       DAFATA010000145         8144 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFATA010000145 DAFATA010000000
VERSION     DAFATA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.146"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1682)
                     /gene="glnE"
                     /locus_tag="LGJ20_004343"
     CDS             complement(<1..1682)
                     /gene="glnE"
                     /locus_tag="LGJ20_004343"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBK2388337.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAA"
     gene            complement(1705..3006)
                     /gene="ygiF"
                     /locus_tag="LGJ20_004344"
     CDS             complement(1705..3006)
                     /gene="ygiF"
                     /locus_tag="LGJ20_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBK2388338.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            3248..3868
                     /locus_tag="LGJ20_004345"
     CDS             3248..3868
                     /locus_tag="LGJ20_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBK2388339.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            3932..5170
                     /gene="cca"
                     /locus_tag="LGJ20_004346"
     CDS             3932..5170
                     /gene="cca"
                     /locus_tag="LGJ20_004346"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBK2388340.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAIASWKEQRCPKPD"
     gene            complement(5333..6154)
                     /gene="bacA"
                     /locus_tag="LGJ20_004347"
     CDS             complement(5333..6154)
                     /gene="bacA"
                     /locus_tag="LGJ20_004347"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK2388341.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(6244..6612)
                     /gene="folB"
                     /locus_tag="LGJ20_004348"
     CDS             complement(6244..6612)
                     /gene="folB"
                     /locus_tag="LGJ20_004348"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBK2388342.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGTRFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            6717..7334
                     /gene="plsY"
                     /locus_tag="LGJ20_004349"
     CDS             6717..7334
                     /gene="plsY"
                     /locus_tag="LGJ20_004349"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK2388343.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(7347..>8144)
                     /gene="ttdR"
                     /locus_tag="LGJ20_004350"
                     /gene_synonym="dan"
     CDS             complement(7347..>8144)
                     /gene="ttdR"
                     /locus_tag="LGJ20_004350"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HBK2388344.1"
                     /translation="NTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQ
                     IKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDI
                     RINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGI
                     WELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLP
                     EYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
BASE COUNT         1941 a         2328 c         2025 g         1850 t
ORIGIN      
        1 gcggcacaca gagaaatcaa atgtttaagc gccgcgggga attcactgag caattctaaa
       61 taggtggtgc gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtaacggca
      121 gcgtcttcac gcgcacagac atcacttagc agatgcggca tcagatggtc gagcacctga
      181 cgtcctcgcg gcccgatggt gcgcttatcc agctctttgc ggaaatcggc aatcagcgtt
      241 agcacctgtt tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt gtcatcttcc
      301 tgcaacgcat cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca
      361 ctttcgtcgt cgccaatcaa ttcattaaac acccggcgca cattggtcat atgtgcggtc
      421 agcgccccgg tcagttgcgg ccagtcagca aagtccatcg cccacgccag ccgcgcacgg
      481 ttaagctcat cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg cagcaggttt
      541 tccagacgcc gcaggaacag atacgccact cgcaattgtt cagcatcgtt ttcagaaagc
      601 agatgcagcg cggcaatgac gctgagcgtt ggcagtaaag agcgcgactg cagcgacggt
      661 tcgcgcccac cgcgaatgag ctggaacacc tgaacgataa attcaatttc gcgaatgccg
      721 cctgcgccga gtttgatatt gtcggtcaaa ccacgtcgac gcacttcacg ggcaatcatc
      781 cctttcatgt tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac
      841 gggcgcagca tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc cataatccgc
      901 gccttgacca tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata atcctccaac
      961 gcggcaaagc tcagcaccag cgggccactt tcgccaaacg gacgcagccg catatccacg
     1021 cgatagacga agccatcctg cgttggttga tccaacactt taatcagccg ctgccccatg
     1081 cgggtaaaaa actgcgcgtt atccagttcc cggcgtccgc cctgcgtaca accatgttcc
     1141 ggccaggcaa aaatcagatc gatatctgag gagaaattca gttccccacc gcccagctta
     1201 cccatgccta aaatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccac
     1261 tcgcggcagc aagcgtcata cagccagtca cgcgcggcga caatcagcgt ctccgccaga
     1321 tggctgagct gctgcaatat gctctcttca gtaaccaaag ccagcgtttg cgcccaggcg
     1381 atgcgcacca taatgcgccg ccggaatagc cgcagctcgc gcattaaccc ggcttcgtca
     1441 ctcacattac tgagcgcctc ctgcaaccat gccgcgtaat gctgccattc gtcggcctgc
     1501 ggcggttggc tttccagttc cgtcagccac tctggatgca caatcacgct gtcctgcaca
     1561 aaatcactaa aagtaagtac tgacttcgcc tgtgcgctaa gtgattcctc ggctaaaggc
     1621 tctggcagcc gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc
     1681 ataagcgatt ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg
     1741 attgtttgcc tcattacgga aatgttcaat ttcaatgcgt tgcccggtcg caatagcgtg
     1801 acgcagcccc tgccagttct ccagccaggc ttgcgcgacg acaggatcat agtaacctgc
     1861 cagcagcagt attgagtcaa tatcacgcgt caggcgtggc aactgatcat ggtagcgatc
     1921 gcctaacggc tggcagaaaa cgcttttcag ttcagcggca tggcgggaaa gatggatatc
     1981 ggcaaagcgt ttgaaggaat cgctgatttt accctgcgct ttggcatcta aaaacggctg
     2041 ccaggctttg cttaccaacc attcggtcaa cgccagcttt gccattgccg tttcggtaga
     2101 gtagaccgcc gtcacggcag aaaccgcaga agcgatggtc gcttcgcatt gagtcagcaa
     2161 atcacgtaag tgagtgctcg ctttacgcgg cacaatgcca ccgaacagca tcagtgcatg
     2221 gcgaacaagg ccaatggctg ccagcacctg ctctttcgcc gcatcgttgc cgcgtaccca
     2281 cagttcttca tgatactgcc attgcgctaa cgccagctcg aacgccgctt ccagcccctg
     2341 ttccacatcg gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg
     2401 attgccctgc gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc
     2461 ggtttgcgat accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag
     2521 ttccagttcc agctcacaga taggttcagc aaattccccc gctttcacct ccccctgatc
     2581 gagagcgatt tcaattcggc tgtcatcgac cgccaccagc catttttcgc gataaaaatc
     2641 ggtgctgaac agcggttgta cgcgggaagc gagatcggcg ggcaattcgc cgttcggcca
     2701 gacttccgtc ggtaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc
     2761 cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg
     2821 accgttttcg ccacgaatac gtaagcccat atcgtgccca cgcagccagt tatccggcgt
     2881 ttcgtagtaa atattcagca actgcacggg gtcatggtgc tcgccgccca gcgtattgag
     2941 atggtcacgc aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg
     3001 agccatggcc tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg
     3061 cgatcttttt tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga
     3121 cgggcgtaaa agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga
     3181 atgctttgcg tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga
     3241 cagcctgatg ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgccactgc
     3301 cgtctcacac gctgaagaaa cgcgctatgt ttctgatgaa ctgaatacct gggtccgtag
     3361 cggtccggga gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt
     3421 attacaaact gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc
     3481 ctggatcccg ttgaaacaac ttagcactga gccaagcctg cgctcccgtg tgccagatct
     3541 ggaaaatcag gtcaaaaccc tgaccgataa actcaccaat atcgataaca cctggaatca
     3601 gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca acggattaaa
     3661 agaagaaaat cagaaattga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc
     3721 cagcgtacag ttggatgaca aacagcgcac catcatcatg caatggttta tgtatggcgg
     3781 tggcgtgctg gggcttggct tgctgctcgg tctggtactg ccacacctga tcccaacgcg
     3841 caaacgcaaa gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta
     3901 ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctgt
     3961 tcgggatgca ttgttagggc taccggtcaa agacagagat tgggtggtgg tcggcagtac
     4021 gccacaggag atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct
     4081 gcatccgcaa acgcatgaag agtatgcgct ggcacgtacc gaacggaaat ccggttccgg
     4141 ttacaccggt tttacttgct atgccgcacc ggatgtcacg ctggaagatg atcttaagcg
     4201 tcgcgatctg accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta
     4261 caacggtctg ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga
     4321 agatccgtta cgcgttctgc gcgtggcgcg ttttgctgcg cgttatgccc acctcggttt
     4381 tcgtattgcc gatgaaactc tgacgctgat gcgcgagatg acccatgcgg gtgaactgga
     4441 acacctgacg cctgaacggg tatggaaaga gacggaaagc gccctcacca cccgcaatcc
     4501 acaggtgttc ttccaggtac tgcgcgattg tggcgcactg cgcgttttat tcccggaaat
     4561 tgacgcactg tttggcgttc cggcccctgc caggtggcat ccggaaatcg acacgggtat
     4621 tcatacctta atgacgctct caatggcggc gatgctgagt ccgcaggtcg atgtccgttt
     4681 cgcaacttta tgccacgatc tcggtaaagg actgacgccg ccagaacttt ggccgcgtca
     4741 tcatggtcac ggtccggcgg gtgtgaaatt agtggaacaa ctctgccagc gtctgcgcgt
     4801 gccaaatgaa attcgcgatt tagccagact ggtggctgag tttcacgatc tcatccacac
     4861 cttcccaatg ctgaacccaa aaactatcgt caaattgttt gattccatcg atgcctggcg
     4921 taaaccgcag cgtgtcgagc aactggcgct gaccagcgag gctgacgtgc gcggcagaac
     4981 cggttttgaa tcagcggact acccgcaagg ccgttggttg cgcgaagcct gggaagtggc
     5041 gcagtcagtg ccgacaaaag cggtcgttga agcgggattt aaaggtgtgg agattcgcga
     5101 ggagctgacc cgacggcgga ttgcggcgat agccagttgg aaggaacaac gttgcccaaa
     5161 gcctgactga gtataaaact aaacgttaac acgaaccatc ccctcgcccc ttcggggaga
     5221 gggttagggt gaggggaaca gaccagcacc ggtgcgaaca atcaccccca ccccggccct
     5281 ctccctacag agcgagggag aaaaccaggc ctgctcccga accttttctc aatcaaaaga
     5341 acacgacgta caccgcagcc gccacaataa agcgataaat ggcgaacggg ataaacgaaa
     5401 tgcgcttaat caattgcagg aaggttttaa tcgctatcag cgccaccaca aaagcggtga
     5461 taaacccaac ggcaaacatc gggatatcgc cggttgtcag gaagccccag cttttgtaga
     5521 gatcgagcgc cgttgcgccc atcatcatcg gtaccgccag caggaacgag aactcggaag
     5581 cagcgtaacg gctcacgccc atcagcatcc cacctgaaat ggtggccccg gaacgggaga
     5641 aaccaggcca cagcgccaga cactggaaac agccaatcat aaacgcctga cgataggtca
     5701 tatcatcaag acccggcgca cgcggctctt tcggcttcag gcattcggcg gcaatcagca
     5761 acaaaccgcc aacgaccagt gcatacatca catttatcgg gttaaacaat gacttaatcg
     5821 tgtcgtggaa caacagcccc agtaccaccg ccggaatcat ccccagcaaa atgtggatca
     5881 gcgttaaacg acctttgctt tctccttcgt gctgcaacgg gcggccaaag tggatgccaa
     5941 tcaggccaaa cagacgccgc cagaacatca ctactaccgc cagaattgat cctaactgga
     6001 tcacaacttc aaaggttttc gccgtgtcgc cctcgaaccc caacaagtga ccgacaataa
     6061 tcatatggcc cgtgctggat accggcagaa attctgtcaa tccttcgacc acacccaata
     6121 ttgccgctat cagcagcgag tgcatatcgc tcatcaataa acccctaaat tattaaaatg
     6181 taccgcttgt ccggactact gcgtatgacc aggttataac cgtttggttt aacagctgta
     6241 aaattaatta ttttctttca gattattgcc acgctcaatg attacgccaa cattcgccgc
     6301 ccgcgccact gcgcctggct tgctgagttt gatacgcacc cacggcgagt tgaagcgtgc
     6361 taacagcagc tccgccacct cttcagccac gcgttccacc agcgcaaaac gcgtcccctc
     6421 gacgtggctg accaccgttt ctgcaatgtc agcgtaactg aggcaatccg ccacatcatc
     6481 acttttcgcc gctttacggt tatcccatgc catttcgata tcgaacacta acttctgttc
     6541 gatggtctgt tcccagtcgt aaacaccaat agtggtgatt accgaaagtt gctctataaa
     6601 tacaatatcc atcacgtcct gcctgctttt tggctaaccg gataccactt ccggcgaaat
     6661 gtgcgtatta tccacagatt catcgttgaa cacgaatttt caaaacggaa cagcttatga
     6721 gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg cggctccatt tccagtgcca
     6781 ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac cagcggctcc ggcaatccag
     6841 gcgcaaccaa tgtgttacgt atcggtggca agggagcagc cgtagcagta ctgattttcg
     6901 acgttctgaa aggaatgttg cccgtctggg gcgcgtatga attaggtgtc agccccttct
     6961 ggctaggctt aattgccatc gccgcctgtc ttggacacat ctggcccgtt ttcttcggat
     7021 ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat cgcacccatt ggctgggatc
     7081 tcaccggagt aatggcggga acctggttac tgaccgtgct attgagcgga tactcgtcgc
     7141 tgggagcgat tgtcagtgca ctgattgctc cgttttatgt atggtggttt aagccacaat
     7201 tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct gcgtcatcat gacaacatcc
     7261 agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa attcaaaaga aagcgcgaaa
     7321 aggatcccga gtgatttctg gtggatctac atgacctgat agccttcatc gggcttgccc
     7381 agccgttgct ggcaccatgc cgccagaaat tccacgcaga cacgtaattt catgctgcga
     7441 tagagcggct cccggtaaac agcccagata ttggcgcttt gtgcatactc cggcaatact
     7501 tgcaccagtt tgccactctc cagaaacggc aacacatccc actcggaacg cagcataatc
     7561 cctttgccct ccagcgccca ttgcaacact atctcgccgc tattggagga aagatgcccg
     7621 ctaaccttca ccgatttttt ctcctgaccg ttccccaact cccatattcc gtgggtcata
     7681 tcgcgctctt tggtcaccag acagtcatga cgacttaatt cctgtaatga ttgtggctgc
     7741 ggatattttt gcagatactc aggtgctgcg cataatattc ttttattttt cgttaacaga
     7801 tgcgcaatat aataatcagg aatttcgtca ttaatacgaa tatccagatc aatattatcc
     7861 tgcactaaat caatttgccg atcgaacaat tcaaaatgca cctgtaactc aggataattg
     7921 cgcataagtt cggtaatggc tggcgcaata tggctgcgcc caaaaccaaa actacagcca
     7981 atacgaatca tcccctccgg gcgcgttttg atttgcgtga cgtcatcgac cagtcgctga
     8041 tattgggtga ggatttccag cgcatgttcg taacaacgtt ggccactttc agtcagcgcc
     8101 acgccgcgag cggagcggtt cagcagcgtt gttgccagcg tatt
//
LOCUS       DAFATA010000146         7932 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFATA010000146 DAFATA010000000
VERSION     DAFATA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.147"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(64..1614)
                     /gene="cueO"
                     /locus_tag="LGJ20_004351"
     CDS             complement(64..1614)
                     /gene="cueO"
                     /locus_tag="LGJ20_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBK2388345.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            1780..2127
                     /locus_tag="LGJ20_004352"
     CDS             1780..2127
                     /locus_tag="LGJ20_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBK2388346.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            2233..3099
                     /gene="speE"
                     /locus_tag="LGJ20_004353"
     CDS             2233..3099
                     /gene="speE"
                     /locus_tag="LGJ20_004353"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBK2388347.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            3115..3909
                     /gene="speD"
                     /locus_tag="LGJ20_004354"
     CDS             3115..3909
                     /gene="speD"
                     /locus_tag="LGJ20_004354"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBK2388348.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(3947..4309)
                     /locus_tag="LGJ20_004355"
     CDS             complement(3947..4309)
                     /locus_tag="LGJ20_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBK2388349.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(4484..7081)
                     /gene="acnB"
                     /locus_tag="LGJ20_004356"
     CDS             complement(4484..7081)
                     /gene="acnB"
                     /locus_tag="LGJ20_004356"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBK2388350.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            7436..>7932
                     /locus_tag="LGJ20_004357"
     CDS             7436..>7932
                     /locus_tag="LGJ20_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBK2388351.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLA"
BASE COUNT         1877 a         2212 c         1927 g         1916 t
ORIGIN      
        1 atttgcgttt gtcatcagtt taatgcccgg agagatccgg gcatatttcc gaatacggtc
       61 tttttatacc gtaaacccta acatcatccc cgtatcttca tgctccagca gatggcagtg
      121 cgccatataa gcatgttctt tcggtgcgtt gtgattaaac ttcaccagca cttcactgac
      181 attgccttct accttaacgg tatctttcca gcccgcgcga tgagccgctg gcggtttgcc
      241 attttctgac aagatacgga actgggtgcc gtgaatatgg aacggatgca gcatcatgtc
      301 gcccacgcca gagataaccc aacgttcgta ctgccctttc gccgccgcaa acatcggctt
      361 attcatatca aacgcctgac cgttgatttt attggcatgg tggaaatcga acttcccgcc
      421 atggttcata tgattcatat tgccgtgccc catatggccc atcatctggc tatgatccat
      481 cccggccatc gcctgatcgc catatttctc catcagcatc tgcatcccca tcatatcgag
      541 catcgggtcc atagaaagtt gcagcttgcg taccgtcagc ccttccagcg aaggtaacgc
      601 aggcaggcta cttaatgtgt ctggcaaagc accggaggca ctaatagcaa tcggctgaat
      661 ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc atccccatct ggctgaccgg
      721 tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc accagcactt caaaacgctc
      781 gcccatcagc accggcagct cgctcacctt caccggttca ggtagcagac caccgtcgct
      841 ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa ttgagcgagc gggcattaca
      901 gccattgagc aaacgcaggc gcagccaacc acgtggggca gcgtgttgtg ggtagattgc
      961 accgttggtc agcagcgtat cgccaaacca gcccacggcg gcggtcatca catccagttg
     1021 ataatcaatc tgcccatcag cgttaaattt tttatcctga acaatcaccg gaacatcatc
     1081 gataccccac tgttttggca gcattaattt caggatctcg tcatcttcaa ttaccaccag
     1141 cccagccaac cccatcgcca cctgtcgccc ggtcttgcca tgttgatgtg gatggaacca
     1201 gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag cgcttgccac ctggcggaat
     1261 aattccctgc gggccgccgt cgacttcacc cggtacttcc agcccgtgcc agtgcaacgt
     1321 cgtctcttcc gtcagttggt tatagatatc aaccgttacc gctttgccgc gctgtaattt
     1381 caccgccggc cccagcagat tgccgttata gccccaggta gttgcagttt tctcgccaaa
     1441 ggtagactga cctgcgccaa tagttaactg aatgcgatta cgggcatcgg tcgtgagcaa
     1501 atcagggatc ggtaacgttg ggcgttccgc cgcaaagact gcgcggctcc acagcggcaa
     1561 ggctgaagcc acacccagcg cgacggaata ttttaagaaa tcacgacgtt gcatagttat
     1621 ttccttattc ttaagcaggc gaaacaaaat caaacgttga gcatagtcct tccccttacg
     1681 ggaaggtcaa gccgcagaca caatttaatc gacaataatg gtcgtcgcct cgctcgcagt
     1741 gtgctaacgt ttatcttctt taagcccctg gtagaagcaa tgaagacgtt tttcagaaca
     1801 gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt acgcgctcag cgagtctgaa
     1861 gccgaagata tggccgattt aacggcagtt tttgtctttc tgaagaacga ttgtggttac
     1921 cagaacttac ctaacgggca aattcgtcgc gcactggtct ttttcgccca gcaaaaccag
     1981 tgggatctca gtaattacga caccttcgac atgaaagccc tcggtgaaga cagctaccgc
     2041 gatctcagcg gcattggcat tcccgtcgct aaaaagtgca aagccctggc ccgcgattcc
     2101 ttaagcctgc ttgcctacgt caaataatcc ctgatacctt tttgtagaaa tattgaccgt
     2161 gcatccgcgg tcaatgttag ctattatgtt gcgccctttt tttacgggtg ttaacaaagg
     2221 aggtatcaac ccatggccga aaaaaaacag tggcatgaaa cgctacacga ccagtttggg
     2281 cagtactttg cggtagataa cgttctgtat catgaaaaga ccgatcacca agatctgatc
     2341 atttttgaga acgctgcatt tggtcgcgta atggcgctgg atggcgtagt acaaaccacc
     2401 gagcgcgacg agtttatcta tcatgagatg atgacccatg ttccgctact ggcccacggt
     2461 cacgcgaaac atgtgctgat tatcggcggc ggcgacggtg ccatgctgcg tgaagtaacc
     2521 cgacataaaa acgttgagtc aatcacgatg gtggaaatcg atgcgggtgt cgtatcgttc
     2581 tgccgtcagt atctacccaa ccataacgcc ggtagctacg acgatccgcg ctttaagctg
     2641 gtgatcgacg atggcgtcaa tttcgttaat caaaccagcc agacctttga tgtcattatc
     2701 tccgactgca ccgatcctat cggtcccggc gaaagccttt tcacttcggc attttatgaa
     2761 ggctgcaaac gttgcctgaa tcctggcggt atcttcgtcg cacaaaacgg cgtctgcttt
     2821 ttacagcagg aagaagccat cgacagccat cgcaaactca gccattactt cagcgacgtt
     2881 ggcttttatc aggcggcgat tccgacctat tacggcggta tcatgacttt tgcatgggcg
     2941 acagataacg acgccttacg ccatctctca accgaaatta ttcaggcgcg ttttctcgct
     3001 tctggcctga aatgccgtta ttacaatccg gcaatccata cggcagcttt tgccttacct
     3061 cagtatctgc aagacgcact ggcttcacag ccgtcctaag gagaagataa gaaattgaaa
     3121 aaactgaaac tgcatggctt taataatctg accaaaagtc tgagtttttg tatttacgat
     3181 atctgctacg ccaaaactgc cgaagagcgc gacggttata ttgcttatat cgatgaactc
     3241 tataatgcca accgtctgac cgaaatcctg tcagaaacct gttccattat cggggccaat
     3301 attcttaaca tcgcccgcca ggattacgaa ccacagggtg ccagcgtcac tattctggtg
     3361 agtgaagaac cggttgaccc gaaactcatc gacaaaacag aacaccccgg cccactgcca
     3421 gaaacggtcg ttgcccatct cgataaaagt catatttgcg tacataccta cccggaaagt
     3481 catcctgaag gcggtttatg taccttccgc gccgatattg aagtctctac ctgcggcgtg
     3541 atttctccgc tgaaggcgct gaattacctg atccaccagc ttgagtccga tatcgtaacc
     3601 attgattatc gcgtgcgcgg ttttacccgc gacattaacg gtatgaagca ctttatcgac
     3661 catgagatta attcgattca gaactttatg tctgacgata tgaaggcgct gtatgacatg
     3721 gtggatgtga acgtctatca ggaaaatatc ttccatacca agatgttgct taaagagttc
     3781 gaccttaagc actacatgtt ccacaccaaa ccggaagatt taaccgacag cgagcgccag
     3841 gaaattaccg ctgcgctgtg gaaagaaatg cgcgagattt attacgggcg caatatgcca
     3901 gctgtttaac ggctctggcg gagctcccag gctccgccag atttatttac ttctgctgca
     3961 cgaaattgcg gtaagccgcc acgacctgca gaaaatcctc aacgccgcat agcgacaggc
     4021 tttcttcgtc gtagtagttc atcccctctt ccatttcatc gccagcgaat tccagttgat
     4081 tggcgcgaac catcacctct tcaccgtcca tccacagggt gtattcatgc cctgcccgtt
     4141 gccaggaccg ttcgctacct ttcagtgcgt gcgctgcttg ttccacttca tcaagcaagg
     4201 ccaggttttc tttcacctct tcattaaacc agtgcccgac cacttcatgc cccatggaca
     4261 tacgcacctt taccactccg gtaatatcgc gcagaaattc gtaatccata gtgtgttcct
     4321 ctgctcccgg caatgcgccg aaatcgcatc gcgccattga tgtaattatc gcagccttac
     4381 cggagaaaaa cagcggaacg gcgatggttt agaaataaaa aatgcccggg aatctcattc
     4441 accgggcatt gtgtcgttta tgcgcagcgc gtgcgcttac ttattacacc gcagtctgga
     4501 aaatcacccc atcggctttt tcggtgtact gagaaagctg gttgaagttc agataacgat
     4561 aagtatcaac ggcagtttta tctacttgcg caacgtaggt ctggtactct tccggcgtcg
     4621 gcagtttgcc aatcagcgcc gcaacagccg ccagttctgc agaagccagg aagacattcg
     4681 cgccagtccc cagacggttc gggaagttac gggtagaggt ggaaaccacc gtcgcaccgt
     4741 ctgctacacg cgcctggtta cccatacaca gagaacagcc cgggatctcg atacgagccc
     4801 cgctcttacc gaagacgctg tagtagcctt cttcagtcaa ctgtgcggcg tccatacggg
     4861 ttggcggtgc cacccacagg cgggtcggca actggccttt atgcgcatcc agcagtttac
     4921 ccgcagcacg gaagtgaccg atgttagtca tgcaagaacc gataaatact tcgtcgatct
     4981 tctcgccctg taccgcagac agcggacgcg cgtcatccgg gtcgttcggt gcacagagga
     5041 ttggctcttt aatatccgcc agatcgatgt cgatcactgc cgcgtattca gcatctgcat
     5101 cggcttccag cagctcagga ttcgccagcc atttttccat gccctgaata cgacgttcga
     5161 gggtacgacg atcgccataa ccttccgcga tcatccactt cagcaggacg atgttagagt
     5221 tcaggtattc gatgatcggt tctttgttca gcttgatagt acaaccagcg gcagaacgct
     5281 cagcggacgc atcggtcagt tcaaatgcct gctcaacttt cagatccggc agaccttcaa
     5341 tttccagaat gcgaccagag aagatgtttt tcttgccttt cttctcaacg gtcagcagac
     5401 cttgtttgat cgcatacagc gggatcgcgt gtaccagatc gcgcagggtg atgcccggct
     5461 gcattttgcc tttgaagcgc accagaacgg attccggcat atccagcggc attacgcctg
     5521 ttgcggctgc aaatgccacc agaccagaac ccgccgggaa agagataccg atcgggaaac
     5581 gggtatggga gtcaccaccg gtaccgacgg tatccggcag cagcatacgg ttcagccagg
     5641 agtggattac gccgtcaccc ggacgcagcg acacaccacc acggttcata atgaagtccg
     5701 gcagcgtgtg gtgcgtgttc acgtcaactg gcttcggata cgccgcggta tggcagaaag
     5761 actgcatcac caggtcagcc gagaagccca ggcacgccag gtctttcagt tcatcacggg
     5821 tcatcgggcc ggtggtgtcc tgagagccta cagaagtcat tttcggttcg cagtacgcgc
     5881 ccggacgaat gcctttcacg ccacaggcac ggcctaccat tttctgcgcc agcgagaagc
     5941 cgcgatcgct ctcagcgaca tctttcgcct gacggaacac atcactgtgc ggcagaccaa
     6001 gtgcttcacg cgctttggtg gtcaggccac gcccgataat cagcggaata cgaccgccag
     6061 cacgcacttc atcaatcagc acgtcggttt tcagttcgaa ggtcgccagc agttcgccgg
     6121 tttcgtggtt acgcacttca cctttgtacg ggtaaacgtc aatcacgtcg cccatgttca
     6181 ggttagagac gtcgacttcg attggcagtg cacccgcgtc ttccatcgtg ttaaagaaga
     6241 tgggtgcaat tttaccgccg aggcacaaac caccgccgcg tttgttcggc acatgtggaa
     6301 tatcatcgcc cataaaccac agaacggagt tagtggcgga tttacgcgaa gaacccgtac
     6361 ccacaacgtc accgacgtac gccagcggga aacctttctg ttgcagagct tcgatttgct
     6421 tgatcggacc aacaacacct ggctggtctg gctcaatacc ttcacgggcg tttttcagca
     6481 tcgccagcgc gtgcagtggg atatccgggc gtgaccacgc atccggtgcc ggagaaaggt
     6541 catcggtgtt agtttcgcca gtgactttga agacggtaac ggtcagtttt tcagccagcg
     6601 ccgggcgatt caggaaccat tcggcatccg cccaggactg cataacctgc ttcgcatatt
     6661 cgttgcctgc tttcgctttc tcttctacgt catagaagtt atcgaacatc agcagcgtgt
     6721 gagaaagtgc tttggcagca ataggtgcca gtttggcatc atccagcgcg tcgatcagcg
     6781 gatgaatgtt gtaaccaccc tgcatggtgc ccagcagttc gatggctttt tccggagtca
     6841 gcagagggga tttggcttcg cctttcgcga tagcagccag gaagcctgct ttgacatagg
     6901 cggcttcatc gacgcctggg ggaacacggt tggttaacag atctaacagg aattcttctt
     6961 cgcccgcggg cgggtttttc agcagctcta caagtgcggc catttggttt gcatccaggg
     7021 gtttgggcgc aatcccctca gcggcacgct cagctacgtg cttacggtat tcttctagca
     7081 cgacggttct cctcgctctc attgtcatag tgcggcaggc gattctcttc acgctcctgt
     7141 gagacagcag tttgtaggat aaatgcccag taccgcaacg ggcagaatag caggattttg
     7201 acgatgtgtt aatctgttta caaaaaagca acataaaaaa gtggctgaat cgttaaggat
     7261 ggtctagaga ttgtttccct ccgtaaaaac ttcgttaatt cgcatggtga taatcacctt
     7321 tctcaacgaa tcccaagatt agtcaaaatt taaactaccg cctctttata ctcggattca
     7381 cagcacctgc gggtggcagt tcgccgacca ttgcgatttc cttgagatcc gaattatgaa
     7441 aatgactttg ccgtttaaac cccatgtgct ggcgctaatt tgcagtgccg ggctttgtgc
     7501 cgcctctacc gggctatata taaaaagccg cacagtggaa gcgcctgtgg aaccgcaatc
     7561 gacacaacag actgcgcctg acatcaccgc agttacgctt cctgcaacgg tttccgcacc
     7621 gcccgtaacg cccgccgtcg tcaaatccgc attcagcact gcacaaatcg atcaatgggt
     7681 tgcgcctgtc gcgctgtacc ccgattctct gctttcacaa gtgttaatgg catcaaccta
     7741 tccggcaaac gttgctcaag cagtgcaatg gtcgcacgat aatccactta aacaaggcga
     7801 tgctgctatt caggcggtat ctgaccagcc gtgggacgcc agcgttaaat cactggtggc
     7861 ctttccacaa ttgatggcat tgatgggcga aaacccgcaa tgggtgcaaa acctgggcga
     7921 tgcttttctg gc
//
LOCUS       DAFATA010000147         7836 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFATA010000147 DAFATA010000000
VERSION     DAFATA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.148"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..614)
                     /locus_tag="LGJ20_004358"
     CDS             complement(<1..614)
                     /locus_tag="LGJ20_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HBK2388352.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPA"
     gene            complement(727..2661)
                     /gene="yeaG"
                     /locus_tag="LGJ20_004359"
     CDS             complement(727..2661)
                     /gene="yeaG"
                     /locus_tag="LGJ20_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBK2388353.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3097..3843
                     /gene="mipA"
                     /locus_tag="LGJ20_004360"
     CDS             3097..3843
                     /gene="mipA"
                     /locus_tag="LGJ20_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBK2388354.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGNHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3933..4787
                     /gene="yeaE"
                     /locus_tag="LGJ20_004361"
     CDS             3933..4787
                     /gene="yeaE"
                     /locus_tag="LGJ20_004361"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBK2388355.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(4838..5722)
                     /locus_tag="LGJ20_004362"
     CDS             complement(4838..5722)
                     /locus_tag="LGJ20_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBK2388356.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(5806..6801)
                     /gene="gapA"
                     /locus_tag="LGJ20_004363"
     CDS             complement(5806..6801)
                     /gene="gapA"
                     /locus_tag="LGJ20_004363"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK2388357.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7143..7556
                     /gene="msrB"
                     /locus_tag="LGJ20_004364"
     CDS             7143..7556
                     /gene="msrB"
                     /locus_tag="LGJ20_004364"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK2388358.1"
                     /translation="MANKPSTEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            7598..>7836
                     /locus_tag="LGJ20_004365"
     CDS             7598..>7836
                     /locus_tag="LGJ20_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBK2388359.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFG"
BASE COUNT         1958 a         1909 c         1966 g         2003 t
ORIGIN      
        1 tgcgcaggtt cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc
       61 cgcttgcctg ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg
      121 ctgatattgg ccggaacgcc gttagcagta taacccgccc gatgcgtttt atattcggtc
      181 agctggcgtt gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcaaatca
      241 agatactcat ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggctg
      301 gcctggccct gaccactgcc ggaacctccg ccgccaccct ggggacgttc aattcggtcg
      361 ttctggacga aatggtcatt gcccggatgc acgcggtggc gcagaccgcc acgcccctga
      421 tgaaacatcg gttcgctaat atcttccgtg ggaatggata cggattcgcc gctgtcgacg
      481 tcagtcaccg aacgcttatt aatggcctcg gagatcgact gtttaatttg cgctttataa
      541 cggcgtaaaa aacgctggcg attcaccatg cttttgtttt tgccgttcag acgccggtca
      601 ataaaccagg tcatatgccc cccgtactac atttgccaac tgtatgctac gcctgtgttg
      661 ccgcttctca ggcccacggt tcgctgtcgt tgtaggcccg gtaacatgtg taccgggcca
      721 gggttgttaa gacgatttac gtacgcgcaa ataccattcg cacagtaaac gcacctgttt
      781 acgggtgtag cctttttcca tcatacggtc gacaaagtcg tcgtgtttct tctgctcgtc
      841 ggttgacgtt ttggcgttaa acgagataac cggcaacagc tcctcggtat tggagaacat
      901 tttcttctcg ataaccgtgc gcagtttttc atagctggtc cagttcggat tgcgtccgct
      961 gttattcgct ctggcgcgca gtacgaagtt aacaatctcg ttgcggaaat cttttggatt
     1021 actgatcccc gccggtttct cgattttctc cagctcggcg ttaagagact cgcggtcaaa
     1081 cagctgcccg gtatccggat cgcgatactc ctgatcctga atccagaaat ccgcgtaggt
     1141 aacataacgg tcgaaaatgt tttgcccata ttcggaatag gattcaaggt aggccgtctg
     1201 gatctctttg ccgataaact cggcatattt cgggatcaga taacctttca ggaactccag
     1261 atagcgttct gcctgctctt gtgggaactg ctcgcgctcg atctgctgtt ccaggacgta
     1321 gaacagatgg accgggtttg ccgccacttc tacatgatcg aagttgaaca cgcgggagag
     1381 gatcttaaac gcaaaacgcg tcgacagacc gttcatccct tcatcgacac cggcgtagtc
     1441 acgatattcc tgatacgact tggctttggg atcggtgtct ttcagacttt cgccatcata
     1501 aacccgcatc tttgaataaa tgctggagtt ttctggctct ttaaggcgcg aaagaatgga
     1561 aaaacgtgac agcgtttcca gcgtgccagg ggcgcagggg gcgtgagtca attcactgtg
     1621 attaagcaat ttctcgtaga ttttgatctc ttcggaaatg cgtaagcaat acggcacctt
     1681 cacgatgtaa acacggtcga ggaaggcttc gttgtttttg ttattacgga aagtgaccca
     1741 ttcggattcg ttcgagtggg cgagaataat cccgttgaac ggcagggcgg agataccttc
     1801 cgtcccgttg tagttacctt cctgagtggc ggttaacagc ggatgcagca cttttatcgg
     1861 tgctttaaac atctcgacaa attccatgat cccttggttg gcgcggcaca gcgcaccgga
     1921 atagccgtag gcgtccgggt cattctgcgc gtagtgttcg agtttacgaa tatcgacttt
     1981 cccaaccagc gcggagatgt cctggttgtt ctcatcaccg ggttccgttt tggcgatagc
     2041 aatttgttgc agaattgacg gccagacctt cactacccgg aacttagtga tatcgccacc
     2101 aaattcatgc aggcgttttg ccgcccatgg cgacatgatg gtgccgagat aacggcgagg
     2161 aatgccatac tctttttcca gaatctgagc atcttcctgc ggattgaaaa gacagaacgg
     2221 atgatcgttg accgggctac gctcaccgtt ggcgctcaat acataaatcg gtacgagctg
     2281 cattaatgat ttcagtcgct cagcaagcga tgatttaccc ccacccacag gccccagcag
     2341 atacaggatt tgtttcttct cttccagccc ctgagccgcg tgtttcagat aagagacaat
     2401 ctgttcaatc gcgtcttcca tgccgtaaaa ctcttcaaat gccggataac gtgcaatgac
     2461 ccggttagaa aagagtcgag aaagtctggg ttcctgggct gtatcgacca tgacaggctc
     2521 accgatagcc atcaatagcc gctcagccgc gttggcataa gcactgcgat cttgccgaca
     2581 agtggtaaga aactcctgca gtgtgaactc ttcgtccttg gcagcttcat atcgctggcg
     2641 atagtgatcg aatatattca tggtatgccg tcctttcgtt ttttagcaca ggttaagagc
     2701 cgttcatatg aatagtagag gctcccggaa gaggtaaact gaacgacgtc tcttgctaaa
     2761 acacagcaac tctcatgcca ttttatcgac gatgagaaca acgtgcccgg tgatacacct
     2821 tcaaaattaa gcgtagatgg caaatggaaa acttgcctgc acggctttct taatttcaat
     2881 ggcatatcaa caactcatcc ataaattttg cataattaat gtaaagacca gggtagccag
     2941 taacgctaaa ttcatttggc tgtaagcgcg gtgtcatccg cgtcaggaaa attaaacagt
     3001 tactttaaaa aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg gtaaactttg
     3061 tcgcccgcta aacatttgtt taaggaatga ttaattgtga ccaaactcaa acttctggca
     3121 cttggagtgc ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa attttccctg
     3181 ggcgcaggcg taggtgtcgt tgagcaccca tataaagatt acgataccga tgtttaccca
     3241 gtaccggtaa tcaactatga aggcgataac ttctggttcc gtggcttagg tggtggttac
     3301 tacctgtgga atgacgcaac ggacaaactt tcaattaccg cttattggtc gccgctttac
     3361 ttcaaagcga aagacagtgg caatcaccaa atgcgtcacc tggatgaccg taagagcacc
     3421 atgatggctg gtctgtctta tgctcacttt acccagtacg gttacctgcg taccaccctg
     3481 gctggcgata ccctggataa cagcaacggc atcgtctggg atatggcctg gttgtatcgt
     3541 tacaccaacg gcggcctgac cgtgaccccg ggtatcggtg tgcagtggaa cagcgaaaac
     3601 cagaacgaat actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg tctgcgtggc
     3661 tataacccga acgacagctg gagcccttac ctggagctga gcgccagcta caacttcctc
     3721 ggcgactgga gtgtttacgg taccgcgcgc tatacccgtc tgtctgatga agttactgac
     3781 agcccgatgg tggataaatc ctggactggc ctgatttcta ccgggatcac ctacaaattc
     3841 tgataatgca tctttccagg gcattttgtg cataaaaaca gggcgtcacg ccctgttttg
     3901 cattacggtg gtgcaatacc tgggaggaaa caatgcaaca aaaaatgatt caatttagtg
     3961 gcgatgtctc actgccagcc atagggcagg gaacatggta tatgggcgaa gatgccagtc
     4021 agcgcaaaac agaagttgct gcactacgcg cgggcattga gctcggttta accctcattg
     4081 ataccgccga aatgtatgcc gatggcggtg ccgaaaaggt ggttggggaa gcattaaccg
     4141 gtctgcgcga taacgtcttt ctcgtctcta aagtctatcc gtggaatgct ggcgggcaaa
     4201 aagcgataaa tgcatgcgaa gccagtttac gccgtctcaa tactgattat ctcgatctct
     4261 acttattaca ctggtctggc agtttcgctt ttgaagagac tgtggcagcg atggaaaaat
     4321 tgatcgccca gggaaaaatc cgccgctggg gcgtttctaa ccttgatcat gctgatatgc
     4381 aggaactctg gcagctgccg gggggaaatc agtgtgccac taatcaggtg ctttaccatc
     4441 tcggttcacg agggattgag tacgatctac tcccctggtg ccagcaacag cagatgccgg
     4501 tgatggctta cagtccatta gcccaggccg ggcggttgcg caatggactg ttaaaaaacg
     4561 cggtagtcaa cgaaattgca catgctcaca atatcagcgc ggcacaagta ttgttggcgt
     4621 gggtaatcag ccatcagggt gtgatggcga ttccaaaagc ggccacgatt gcccatgtcc
     4681 aacagaatgc ggctgtgctt gaggtcgaac tttcgtcagc ggaattaacc atgctggata
     4741 aagcgtatcc ggcaccaaaa ggaaaaactg cgctggatat ggtgtgatgt tgatacgtgc
     4801 cggatgcatc gcacccggca cacaacatta acgcaaatta acgtttcgca acgcgaatgg
     4861 attgcgccag atgtgcaggt ttctctttgg tcactttttg cgtttctgaa gcgtaagccg
     4921 tttctacaca aacaaatgtt ttgtagccat catccggcat gtcgcccatg ctaactgaaa
     4981 gcgccggtcc cgggttccag ccgacaacgt tcagatgatg ctggtggcct acggcgataa
     5041 tacgattcag cgcttcatca ttaatcacgc tgcaatcttg cggattcaga tacacgcgat
     5101 cggtacggtc agggaaggtc tgaataccgt cggtcagtac atcttctttc gcgtcattta
     5161 ctttatcaat gaagcgatcg cccagcccac tgacgcttac cttagcgata tcacccacgt
     5221 taaagtaggt atgcagggca gaggtggttt caaattcgcc atgtgattca agatcgattt
     5281 cacaggtttt acccacgcgg aaatgcgcta acagcgtaaa gtcgtgcggc cagaattttt
     5341 tcgtctcttc gctttgcgtc aattcaaaag tcagcgctac gccatcagca tcttcacgat
     5401 gtgatttcag cgtccacggc aggttgcggg caaaaccgtg cgcaggcaga ccttgttgtg
     5461 ccgccggacc aaaccacggc cagcaaaccg gtacgccacc gcgaatagcg acgccatttt
     5521 tgaacggtgt gttgttgctc aaccacagaa cttcttcttc acccgcaggt ttccacgaga
     5581 gaaggtgtgc gccctgtaat gcaaaagagg cttttacctg gggatgatcg accacaatga
     5641 ggtccagttc atccagttta cgacgggaga ggacagggga gatttgttcg atgaccggaa
     5701 gggcaaaaat tttcttaatc atgacgcagt cctttaactt cattttatca ggtaaaaaaa
     5761 agagcgaccg aagtcgctct ttttagatca cagtgtcatc tcaacttatt tggagatgtg
     5821 agcgatcagg tccagaactt tgttggagta accggtttcg ttgtcgtacc aggataccag
     5881 tttcacgaag ttgtcgttca gagcgatacc agctttagca tcgaacacgg aagtgcaaac
     5941 ttcgccgttg aaatcggtag atactacgtc atcttcggtg tagcccagaa cgcctttcat
     6001 ttcgccttca gcagcagctt taacggcagc tttgatctgc tcgtaagttg cagctttttc
     6061 cagacgaacg gtcaggtcaa ctacggatac gttcggggtc ggaacgcgga acgccatacc
     6121 agtcagtttg ccattcagtt ctggcagtac tttacctaca gctttagcag caccggtaga
     6181 ggacgggatg atgttctggg aagcgccgcg gccgccgcgc cagtctttgt gagacgggcc
     6241 atcaacggtt ttctgagtag cggtagtagc gtgaacggtg gtcatcagac cttcgatgat
     6301 gccgaagtta tcgttgataa ctttagccag cggagccagg cagttggtgg tgcaggaagc
     6361 gttggaaacg atgtcctggc cagcatattt gtcgaagtta gcgcctttaa cgaacatcgg
     6421 agtgttgtct ttagacggac cagtcataac cactttcttc gcaccagcgg tgatgtgttt
     6481 acgagcagtt tcgtcagtca ggaacagacc agttgcttca gcgacaacgt caacaccaac
     6541 ttcgtcccat ttcaggttag ccggatcacg ttcagcggta acacggattt ttttaccgtt
     6601 aacgatcaga tgaccgtctt tcacttcaac ggtaccgtcg aaacggccgt gagtggagtc
     6661 atatttcagc atgtatgcca tgtaatcagc gtctaacagg tcgttgattg caacgatctc
     6721 gatgtcagaa cgtttctgag cagcacggaa aacaatgcga ccgatacggc caaaaccgtt
     6781 gatacctact ttgatagtca tatattccac cagctatttg ttagtgaata aaaggttgcc
     6841 tgtaaaatta caaaaacctt acgcagcgtc aagcggaatc gtgtcaatca ttgcgacaaa
     6901 tcaatcctgt gcctaagcat tacgcgactg actcgcctca ctcttccttt gggcttgaga
     6961 ccacatgggg tcggcgcccc gaattttaaa gggcaattac gataaaaatg tgattttcat
     7021 cacgatttag gtgcagccat ttcgtcgaga tcaagtttag gacaaaattt tgccagttgg
     7081 tgttaatgtt ttgttagaat cagtcacgta atgtgagcac gattaaagtg agatgtgagc
     7141 aaatggctaa taaaccttcg acagaagaac tgaaaaaaaa tttgtccgag atgcagtttt
     7201 acgtgacgca gaatcatggg acagaaccgc catttacggg tcgtttactg cataacaagc
     7261 gtgacggcgt atatcactgt ttgatctgcg atgccccgct gtttcattcc gaaaccaagt
     7321 atgattccgg ctgtggctgg cccagtttct acgaaccggt gagtgaagaa tccattcgtt
     7381 atatcaaaga cttgtcacat ggaatgcagc gcatagaaat tcgttgcggt aactgtgatg
     7441 cccatctggg gcatgtcttt cccgacgggc cgcaaccaac aggcgaacgt tattgtgtta
     7501 actctgcttc tttacgcttt accgatggcg aaaacggcga agaaatcaac ggttgaaaaa
     7561 acgattcagc aacttattcc acaggagcgg aaaaattatg aatcttgatg acattatcaa
     7621 cagcatgacg cctgaggtat accagcgttt gtcgaccgcc gttgaactgg ggaaatggcc
     7681 tgatggcgtt gcgttgaccg aggaacaaaa agagaattgc ctgcaactgg tgatgctgtg
     7741 gcaagcccgc cataataccg aagcacagca tatgacaatt gacacaaatg gtcagatggt
     7801 catgaagagc aaacagcagt taaaagaaga ttttgg
//
LOCUS       DAFATA010000148         7811 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFATA010000148 DAFATA010000000
VERSION     DAFATA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.149"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(105..2495)
                     /gene="gcd"
                     /locus_tag="LGJ20_004366"
     CDS             complement(105..2495)
                     /gene="gcd"
                     /locus_tag="LGJ20_004366"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBK2388360.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            2701..3237
                     /gene="hpt"
                     /locus_tag="LGJ20_004367"
     CDS             2701..3237
                     /gene="hpt"
                     /locus_tag="LGJ20_004367"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK2388361.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            complement(3278..3940)
                     /gene="can"
                     /locus_tag="LGJ20_004368"
     CDS             complement(3278..3940)
                     /gene="can"
                     /locus_tag="LGJ20_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBK2388362.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            4049..4975
                     /locus_tag="LGJ20_004369"
     CDS             4049..4975
                     /locus_tag="LGJ20_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2388363.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            4972..5742
                     /locus_tag="LGJ20_004370"
     CDS             4972..5742
                     /locus_tag="LGJ20_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2388364.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            5847..6287
                     /locus_tag="LGJ20_004371"
     CDS             5847..6287
                     /locus_tag="LGJ20_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK2388365.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            6351..7580
                     /locus_tag="LGJ20_004372"
     CDS             6351..7580
                     /locus_tag="LGJ20_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK2388366.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     TLDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVLDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(7584..>7811)
                     /gene="panD"
                     /locus_tag="LGJ20_004373"
     CDS             complement(7584..>7811)
                     /gene="panD"
                     /locus_tag="LGJ20_004373"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBK2388367.1"
                     /translation="GKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPD
                     EEARTWRPNVAYFEGDNEMKRTAKAIPVQVA"
BASE COUNT         1844 a         1915 c         2085 g         1967 t
ORIGIN      
        1 agctgtgcaa tccattgatt ttgcacaatt ttgtaggctg gataaggcgt tcacgccgca
       61 tccggcatga acaacgcgca cgttgtcagc aatctgagca agtcttactt cacatcatcc
      121 ggcagcgcat aagccacaat atagtcgccc atcttcgtac caaatgaacc gtgaccgcct
      181 gcggagatca ccacatactg cttaccattc acttcatagg tcattggcgt agcctgacca
      241 cccgctggta aacgaccctg ccacagtttt tcaccgttgc tcatgttgta agcgcgcagg
      301 tagttatctg ccgtagcggc gataaacagc acgttacccg ccgtggagat tggcccgccc
      361 agcatcggca tacccatatt gaacggcacc ggtacaggca tcgggaacgg catactgtcc
      421 tgcggcgtac caatacgttt cttccacacc acttcattgg ttttcagatc cagcgccgag
      481 atataacccc atgctggctg tttacatggc agaccaaacg gtgagaggaa cgggttgagc
      541 gtgacaccat acggtacacc gtactgtggc tgaatgccgg attccgtacc cgtgcctttg
      601 gcatctttcg gctgctccat cgggttaccc ggaccgcgtg ggatcagttt cgaaacaaac
      661 ggcagtgcca ttgggttggc aattgccact tcacgatttg gatcaacgga aatcccgccc
      721 cattcaaaca ttcccaggtt acccgggaag accagcgtgc cctgttcaga tggcggcgtg
      781 aaaatgcctt catagcgcaa ctggtggaac atcacgcggc acaccagttg gtcaaacatg
      841 gtggctcccc acatatccgc accgctcaaa tctttcttcg gacggaagct cagttcagag
      901 aacggctggg tcggagtaac gtaatcgcct ttcgcagcac cttgcggcac cggtttttcc
      961 ggggccggaa ccaccagttc gccattgcga cgatcgagca caaaaatgtt gccggttttc
     1021 gccggagcgt aaataactgg cactttctga ccattaacgg tgatgtccgc cagcgtcggc
     1081 tgtgccggaa gatccatgtc ccacaggtcg tggtgaacgg tctggtagct ccacgccagt
     1141 ttcccggtag tggcattcag cgccagaatc gagctggcat aacgttcctg ttccggtgtg
     1201 cggttaccgc cccagatatc cggcgtggtc acgcccatcg gcagatagac cagatccagc
     1261 ttcgcgtcat aggccgctgg tgcccaggag tttggcgagt taaaggtaaa ggtgtgttcg
     1321 tcagacggga ttgcgttcgg atctttcgcg ccgggatcaa aagcccacag cagctccccg
     1381 gtgttgacat caaaaccacg gatcacgcca gacgtttcgc gggttgagaa gttatcggta
     1441 actgaaccgg ccatcacgat ggttttatcg gtgataatcg gtggcgaagt cggttcatac
     1501 agacccggtt tggtgtctgg catattgctt tgcagattga gcacgccttt attggcgaag
     1561 gtttcgcaca gtttgccgtt ttcagcgtta atcgcaatca gtcgaccatc attgaccgga
     1621 agaatgatac gacgcgggca atccgccatc acttccggcg aagccgtttc tgctttggct
     1681 tcatgataag agacgccacg gcaggttacg tgctggaaag actcgttagt tttcagctca
     1741 ggatcgtaat gccatttctc tttgccgctg gcggcatcga gcgcaaacag gcgctggtga
     1801 gcggtacaca ggtaaagggt gtcgcccact ttaatcggcg tcacttcatt ggtgatttcg
     1861 cccggatcgt tcggctgctt cacatcgcca gtacggaaca cccaggcttc tttcagatta
     1921 tggacgttat cggcgttaat ttgtttcaga ggtgaaaagc gttgaccttc ctgattgcga
     1981 ccataggcag gccagtcctg atcggcaacg ggtgagatag cttccgcagg tgtggcattg
     2041 gcgcttaagg tgccgttgat ttcctgcgga tcgttaaatc cggcccaggt caggatacca
     2101 ccgctaatca gcagtgccac caccagtgcg gcaactgcgc cgctggcagg aatgaccagg
     2161 cgacgccaga caaacggcag gatcagccag atgccgaaga agaccagaat gtcgctgcgc
     2221 ggagtcagcg cccagaagtc gaaaccaact tcccagacgc cccaaatcat ggtgccgagc
     2281 agcagggctg cgtacagcca aagcgcggca cgtttactgc gccacagcat ccaggcgacg
     2341 ccgagcatca caagaccagc gatagggtag taccaggagc cgccaatcgc gaccagccag
     2401 cctccgccaa tgagtagata cagcccgcaa agcgctgcaa aaagggctgt tagcgtgacg
     2461 agtaatcgtc gcgagcctgt attgttaatt gccataaaga gacaccattt caattcatta
     2521 atattttagt agcaattaat tataggtttt aacatgtgtg atcgtcatca caattcgagc
     2581 tttattaaca gattccgcga atgaatagtt ttactggtat actgcgtgtc ttgcgctttg
     2641 ttgcggtgcc aaaacctgcc cgtgcgaagt gatttgtttt taaatcatat ggttagagat
     2701 atgaaacata ctgtagaagt aatgatcccc gaagcggaga ttaaagcgcg tatcgccgaa
     2761 ctgggtcgtc agattactga gcgttacaaa gacagcggca gcgatatggt gctggttggt
     2821 ctgctgcgtg gctcatttat gtttatggcg gacctgtgcc gtgaagttca ggtatctcat
     2881 gaagtcgact ttatgaccgc ctccagctac ggtagcggca tgtccaccac ccgtgatgtg
     2941 aaaatcctca aagatctgga tgaagatatc cgtggcaagg acgtgctgat tgttgaagat
     3001 atcatcgact cggggaatac actgtcgaaa gtgcgtgaga tcttaagcct gcgcgaaccg
     3061 aagtcgctgg cgatttgtac gctgctggat aaaccgtccc gtcgtgaagt gaacgtcccg
     3121 gtagaattta tcggtttctc gatcccggat gagtttgtgg tgggttacgg cattgattac
     3181 gcacagcgtt accgtcatct gccgtatatc ggcaaagtga ttctgctgga cgagtaagtg
     3241 tgaagttgcc ggatgtgttg catccggcat ggcattttta tttgtggttg gcgtgtttca
     3301 gcttgaggtt ggaaatcccg tgacggtaac gttgctcaag ggtttcgcgg ttggtcgctg
     3361 tgacatccag atcacgcagc aagccgtcgt gaatgccgta ggcccagccg tgaatggtaa
     3421 ctttctgccc gcgtttccac gctgattgca taatggtgga gtggcccagg ttatacacct
     3481 gttccatgac gttcagttca cacaaggtat ccagacggcg ctcttgcggc atttcgccga
     3541 gcaatgagct atgtttgaac cagatatcgc ggatatgcag cagccagttg ttgataagcc
     3601 ccagttccgg gttttcaact gcggcttgta cgccgccgca accgtagtgg ccacagataa
     3661 taatgtgttc aacttcgagt acatccactg catactgaac cacggaaagg cagttcaggt
     3721 cagtgtgaat gaccaggtta gcaacattac ggtgaacaaa gagttcgccc ggctcaagac
     3781 cggttaaacg ttctgcagga acgcgactgt cggaacatcc aatccataga aagcgcggtt
     3841 tttgcgcttg tgccagtttc tcaaaaaacc cgggatcctc ttccaccagc atttttgacc
     3901 atagtgcatt gttgctgatg agtgtatcta tgtctttcat ggaggttaac gacctgtaac
     3961 caaataatta cgtttggcta atatagggca actccgggac gatttaaacc acagataaag
     4021 tgtaagaacg taaggtaagt aaaaatttat gaccattgca ctggaacttc aacagcttaa
     4081 aaaaacctat ccaggcggcg ttcaggcgct tcgtgggata gatttgcagg tcgaagcggg
     4141 tgatttttat gcgcttctcg ggccgaacgg ggccgggaaa tcgaccacta tcggtattat
     4201 cagctctctg gtaaataaaa cctccgggcg ggtcagcgta tttggttacg atctcgagaa
     4261 ggatgtcgtg aacgctaaac gtcagttggg actggtgccg caggaattta acttcaaccc
     4321 gtttgaaacc gtgcagcaaa ttgtggtgaa tcaggcaggg tactatggcg tggagcgcaa
     4381 agaagcgtac atccgcagcg aaaagtatct taaacaactc gatctatggg gaaaacgcaa
     4441 cgaacgtgcg cgtatgttat ctggcgggat gaagcgccgt ttaatgattg cccgtgcgtt
     4501 aatgcatgaa cctaaactac tgattctcga cgaaccgacc gcaggcgtgg atattgaact
     4561 tcgccgctca atgtggggct ttttgaagga tttaaacgac aaaggcacca ccatcattct
     4621 caccacacac tacctggaag aagcagaaat gctgtgccgc aatatcggca ttattcaaca
     4681 cggtgagctg gtggaaaata cctcgatgaa ggcgctgctg gcgaagctga aatcggaaac
     4741 ctttattctc gatctcgcac cgaaaagtcc gttaccgaag ctcgatggct atcagtatcg
     4801 actggtcgat accgcgacgc tggaagttga agtgctgcgt gagcagggga tcaacagcgt
     4861 atttacgcag ttaagtgagc agggcattca ggtattaagt atgcgtaaca aagctaaccg
     4921 tctggaagag ctgtttgttt cactggttaa tgaaaaacaa ggagatcgcg catgatgcat
     4981 ctttactggg tggcgctaaa aagcatctgg gcgaaagaga tccatcgctt tatgcgtatc
     5041 tgggtgcaga cgctggtgcc gccagtcatc accatgaccc tttactttat tatcttcggt
     5101 aacctgattg gttcgcgtat tggcgatatg catggcttca gctatatgca gttcatcgta
     5161 ccggggctga tcatgatgtc ggtgatcacc aatgcctacg ccaacgttgc gtcatcattt
     5221 tttggtgcca agttccagcg taatattgaa gagctgctgg tagcgccggt tccgactcac
     5281 gtcattattg ccggatatgt cggcggtggc gtggcgcgtg gtctgtttgt tggcattctg
     5341 gtgacggcaa tttcactgtt ttttgtgccg tttcaggtgc attcgtgggt attcgttgcc
     5401 ttaaccctgg tgctcacggc ggtgttgttc tcccttgcgg gtttgctgaa cggtgtgttt
     5461 gccaaaacgt tcgatgacat cagcctggtg ccaacctttg tgttaacgcc actcacgtat
     5521 ttgggtgggg tcttttactc actgactttg ttgccgccgt tctggcaagg gctgtcgcac
     5581 ctgaacccaa tcgtttatat gatcagtggt ttccgctacg gcttcctcgg tatcaatgat
     5641 gttccgctgg tcactacttt tggcgtactg gtggtcttta ttgtggcgtt ttatttgatc
     5701 tgttggtcgc tgatccaacg tggacgtggt ttgcgtagct aaggctattt cctctcctct
     5761 ggatttgggg gagaggagtt ttgacggcta tcacccttta tcaacaatgg tcagggtaga
     5821 ctgattttcg gctaaggagg aaggcgatgt taggttgggt aattacctgt cacgatgacc
     5881 gggcgcaaga gatactggat gcgctggaga aaaaacatgg ggcgcttctt cagtgccggg
     5941 ccgtgaattt ctggcgtggg ttaagctcta atatgctcag ccgcatgatg tgcgatgctc
     6001 tgcatgaagc ggactctggt gagggtgtca tcttcttaac cgatatagcc ggagcgccac
     6061 cgtaccgcgt ggcttcatta ttaagccaca aacactcccg ttgcgaagtg atttctggtg
     6121 tcacgttacc gttaattgaa cagatgatgg cttgccgtga aaccatgacc agttcagagt
     6181 ttcgcgagcg tattgtcgaa ctgggtgggc cggaggtgag tagtctttgg caccaacaac
     6241 aaaaaaatcc gcctttcgtc ctcaaacata atttgtatga gtattaaccc acgattctta
     6301 tagcgctttt gctacaataa aagcgttgtt tcaccctcgg ttattttttc atgtacaaac
     6361 aagctgttat tctcctgctg atgctgttta ccgcaagtgt cagtgccgcg ttacctgccc
     6421 gttatatgca aaccatcgaa aatgctgcga tctgggcgca aattggcgac aagatggtga
     6481 ccgtggggaa tattcgggcc gggcaaatca ttgccgtgga gcccactgcc gcaagttatt
     6541 acgcatttaa ttttggcttt ggtaaagggt ttatcgataa agggcatctc gagccagttc
     6601 aggggcgaca aaaagttgaa gacggtttgg gtgacctcaa caagccgctg agtaatcaga
     6661 acttaattac ctggaaagat acgccggtct ataacgcgcc gagtgcgggc agtgcgccat
     6721 ttggggtact ggcggacaat ttgcgctacc cgattttgca taaactgaaa gacaggttaa
     6781 atcaaacctg gtatcagatc cgtattggcg atcgactcgc ctatatcagc acgctggatg
     6841 cccaacccga taatggcctg ccggtgctaa cctatcacca tattctgcgc gatgaagaaa
     6901 acacccgttt tcgccatact tcgacgacca cctcggtacg cgctttcaat aaccagatgg
     6961 cctggctgcg cgacagggga tacgcgacac tgagcatggc gcagctggaa ggttacgtga
     7021 agaataagat caatctccct gcgcgagcgg tggtgattac ctttgatgat ggcctcaagt
     7081 cggtgagccg ctatgcgtat cctgtattga aacaatatgg catgaaggcg acggcgtttg
     7141 ttgttacctc gcgcatcaaa cgtcacccgc agaagtggaa cccaaaatcg ctgcaattta
     7201 tgagcgtttc tgagcttaac gaaattcgcg atgttcttga ttttcagtca catacccatt
     7261 ttttgcatcg ggtggatggc tatcgccgac ccatattact gagccgtagt gagcacaata
     7321 ttctgtttga ttttgcacgt tcacgccgcg ctctggcgca atttaatccg catgtctggt
     7381 atctttcgta tccgtttggc ggatttaatg acaaagccgt gaaggcagca aacgatgccg
     7441 gatttcacct ggcggtgaca accatgaaag gcaaagtaaa accgggggat aatccgttgt
     7501 tactaaaacg actttatatc ttaagaacgg attcgctgga gacgatgtcg cggctggtga
     7561 gtaaccagcc gcagggataa caatcaagca acctgtaccg gaatcgcttt cgcggtacgt
     7621 ttcatttcat tgtcgccttc aaaataggcg acattgggtc gccaggtgcg agcttcttca
     7681 tctggcatgg taacgaagct ggcgatgatg acaatatcgc cgacgctggc gcagtgggcc
     7741 gccgcaccgt taacagaaat aattctcgaa ccgcgttctg ccgcgatggc ataagtggag
     7801 aaacgcttgc c
//
LOCUS       DAFATA010000149         7569 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFATA010000149 DAFATA010000000
VERSION     DAFATA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.150"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..840
                     /gene="murG"
                     /locus_tag="LGJ20_004374"
     CDS             <1..840
                     /gene="murG"
                     /locus_tag="LGJ20_004374"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK2388368.1"
                     /translation="PLRIFNAWRQARAIMKAYKPDVVLGMGGYVSGPGGLAAWSLGIP
                     VVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGNPVRTDVLALPLPQQRLAG
                     REGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQSGKGSQQSVEQAYAEAGQP
                     QHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPALFVPFQHKDRQQYWNALP
                     LEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERARAASIPDATERVANEVSRA
                     ARA"
     gene            894..2369
                     /gene="murC"
                     /locus_tag="LGJ20_004375"
     CDS             894..2369
                     /gene="murC"
                     /locus_tag="LGJ20_004375"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK2388369.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            2362..3282
                     /gene="ddlB"
                     /locus_tag="LGJ20_004376"
     CDS             2362..3282
                     /gene="ddlB"
                     /locus_tag="LGJ20_004376"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK2388370.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            3284..4114
                     /gene="ftsQ"
                     /locus_tag="LGJ20_004377"
     CDS             3284..4114
                     /gene="ftsQ"
                     /locus_tag="LGJ20_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBK2388371.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            4111..5373
                     /gene="ftsA"
                     /locus_tag="LGJ20_004378"
     CDS             4111..5373
                     /gene="ftsA"
                     /locus_tag="LGJ20_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK2388372.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            5434..6585
                     /gene="ftsZ"
                     /locus_tag="LGJ20_004379"
     CDS             5434..6585
                     /gene="ftsZ"
                     /locus_tag="LGJ20_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK2388373.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            6686..>7569
                     /gene="lpxC"
                     /locus_tag="LGJ20_004380"
     CDS             6686..>7569
                     /gene="lpxC"
                     /locus_tag="LGJ20_004380"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBK2388374.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELP"
BASE COUNT         1790 a         1774 c         2256 g         1749 t
ORIGIN      
        1 ccgctgcgta tcttcaacgc ctggcgtcag gcgcgggcga ttatgaaagc gtacaaacct
       61 gacgtggtac tcggtatggg cggctatgta tcaggtccag gtggtctggc cgcgtggtcg
      121 ttaggcattc cggttgtact tcatgaacaa aacggtattg cgggcttaac caataaatgg
      181 ctggcgaaga ttgccaccaa agtgatgcag gcgtttccag gcgctttccc taatgcggaa
      241 gtggtgggta acccggtgcg taccgatgtg ctggcgctgc cgttgccgca gcaacgtttg
      301 gctggacgtg aaggtccggt tcgtgtgctg gtagtgggtg gttcccaggg cgcacgcatt
      361 cttaaccaga caatgccgca ggttgctgcg aaactgggtg attcagtcac tatctggcat
      421 cagagcggta aaggttcgca acaatccgtt gaacaggcgt atgccgaagc ggggcaaccg
      481 cagcataaag tgacggaatt tattgatgat atggcggcgg cgtatgcgtg ggcggatgtc
      541 gtcgtttgcc gctccggtgc tttaacggtg agtgaaatcg ccgcggcagg actaccggcg
      601 ttgtttgtgc cgtttcaaca taaagaccgc cagcaatact ggaatgcgct accgctggaa
      661 aaagcgggcg cagccaaaat tatcgagcag ccacagctta gcgtggatgc tgtcgccaac
      721 accctggccg ggtggtcgcg agaaacctta ttaaccatgg cagaacgcgc ccgcgctgca
      781 tccattccgg atgccaccga gcgagtggca aatgaagtga gccgggctgc ccgggcgtaa
      841 ttgtagcgat gccttttgca tcgtatgaat ttaagaagtt aatggcgtaa agaatgaata
      901 cacaacaatt ggcaaaactg cgttccatcg tgcccgaaat gcgtcgcgtt cggcacatac
      961 attttgtcgg catcggtggt gccggtatgg gcggtattgc cgaagttctg gccaatgaag
     1021 gttatcagat cagtggttcc gatttagcgc caaatccggt cacgcagcag ttaatgaatc
     1081 tgggagcgac gatttatttc aaccatcgcc cggaaaacgt acgtgatgcc agcgtggtcg
     1141 ttgtttccag cgccatttct gccgataacc cggaaattgt cgcagctcat gaagcgcgta
     1201 ttccggtgat ccgtcgtgcc gaaatgctgg ctgagttaat gcgttttcgt catggcatcg
     1261 ccattgcagg aacgcacggc aaaacgacaa ccaccgcgat ggtttccagc atctacgcag
     1321 aagcggggct cgacccaacc ttcgttaacg gcgggctggt aaaagcggcg ggggttcatg
     1381 cgcgtttggg gcatggtcgg tacctgattg ccgaagcaga tgagagtgat gcatcgttcc
     1441 tgcatctgca accgatggtg gcgattgtca ccaatatcga agccgaccac atggatacct
     1501 accagggcga ctttgagaat ttaaaacaga cttttattaa ttttctgcac aacctgccgt
     1561 tttacggtcg tgcggtgatg tgtgttgatg atccggtgat ccgcgaattg ttaccgcgag
     1621 tgggacgtca gaccacgact tacggcttca gcgaagatgc cgacgtgcgt gtagaagatt
     1681 atcagcagat tggcccgcag gggcacttta cgctgctgcg ccaggacaaa gagccgatgc
     1741 gcgtcaccct gaatgcgcca ggtcgtcata acgcgctgaa cgccgcagct gcggttgcgg
     1801 ttgctacgga agagggaatt gatgacgagg ctattttacg tgcgctggag agcttccagg
     1861 ggactgggcg ccgttttgat ttcctcggtg aattcccgct ggagccagtg aatggtaaaa
     1921 gcggtacggc aatgctggtc gatgactacg gccaccaccc gacggaagtt gacgccacca
     1981 ttaaagcggc gcgcgcaggc tggccggata aaaacctggt aatgctgttt cagccgcacc
     2041 gttttacccg tacgcgcgac ctgtatgatg atttcgccaa tgtgctgacg caggttgata
     2101 ccctgttgat gctggaagtg tatccggctg gcgaagcgcc aattccggga gcggacagcc
     2161 gttcgctgtg tcgcacaatt cgtggacgtg ggaaaattga tcccattctg gtgccggacc
     2221 ctgcgcaggt agccgagatg ctggcaccgg tattaaccgg taacgacctg attctcgttc
     2281 agggggctgg taatatcgga aaaattgccc gttctttagc tgaaatcaaa ctgaagccgc
     2341 aaactccgga ggaagaacaa catgactgat aaaatcgcgg tcctgttggg cgggacttcc
     2401 gctgagcggg aagtttctct gaattctggc gcagcggtgt tagccgggct gcgtgaaggc
     2461 ggtattgatg cgtatcctgt cgacccgaaa gaagtcgacg tgacgcaact gaagtcgatg
     2521 ggttttcaga aagtgtttat cgcgctacac ggtcgcggcg gtgaagatgg tacgctgcag
     2581 ggcatgctcg agctgatggg cttgccttat accggcagcg gagtgatggc atctgcgctt
     2641 tcaatggata aactacgcag caaactgctg tggcaaggtg ccggtttacc ggtcgcgccg
     2701 tgggtagcgt taacccgcgc agagtttgaa aaaggcctga gcgataagca gttagcagaa
     2761 atttctgctc tgggtttgcc ggttatcgtt aagccgagcc gcgaaggttc cagtgtggga
     2821 atgtcaaaag tagtagcaga aaatgctcta caagatgcat taagattggc atttcagcac
     2881 gatgaagaag tattgattga aaaatggcta agtgggccgg agttcacggt tgcgatactc
     2941 ggtgaagaaa ttttaccgtc aatacgtatt caaccgtccg gaaccttcta tgattatgag
     3001 gcgaagtatc tctctgatga gacacagtat ttctgccccg caggtctgga agcgtcacaa
     3061 gaggccaatt tgcaggcatt agtgctgaaa gcatggacga cgttaggttg caaaggatgg
     3121 ggacgtattg acgttatgct ggacagcgat ggacagtttt atctgctgga agccaatacc
     3181 tcaccgggta tgaccagcca cagcctggtg ccgatggcgg cacgtcaggc aggtatgagc
     3241 ttctcgcagt tggtagtacg aattctggaa ctggcggact aatatgtcgc aggctgctct
     3301 gaacacgcga aatagcgaag aagaggtttc ttctcgccgc aataatggaa cgcgtctggc
     3361 ggggatcctt ttcctgctga ccgttttaac gacagtgttg gtgagcggct gggtcgtgtt
     3421 gggctggatg gaagatgcgc aacgcctgcc gctctcaaag ctggtgttga ccggtgaacg
     3481 ccattacacg cgtaatgacg atatccggca gtcgatcctg gcattgggtg agccgggtac
     3541 ctttatgacc caggatgtca acatcatcca gacgcaaata gaacaacgcc tgccgtggat
     3601 taagcaggtg agcgtcagaa agcagtggcc tgatgaattg aagattcatc tggttgaata
     3661 tgtgccgatt gcgcggtgga atgatcaaca catggtagac gcggaaggaa acaccttcag
     3721 cgtgccgcca gatcgcacca gcaagcaggt gcttccaatg ctgtatggcc cggaaggcag
     3781 cgccaatgaa gtgttgcagg gctatcgcga aatggggcag atgctggcaa aggacagatt
     3841 tactctgaag gaagcggcga tgaccgcgcg gcgttcctgg cagttgacgc tgaataacga
     3901 tattaagctc aatcttggcc ggggcgatac gatgaaacgt ttggctcgct ttgtagaact
     3961 ttatccggtt ttacagcagc aggcgcaaac cgatggcaaa cggattagct acgttgattt
     4021 gcgttatgac tctggagcgg cagtaggctg ggcgcccttg ccgccagagg aatctactca
     4081 gcaacaaaat caggcacagg cagaacaaca atgatcaagg cgacggacag aaaactggta
     4141 gtaggactgg agattggtac cgcgaaggtt gccgctttag taggggaagt tctgcccgac
     4201 ggtatggtca atatcattgg cgtgggcagc tgcccgtcac gtggtatgga taaaggcggg
     4261 gtgaacgacc tcgaatccgt ggtcaagtgc gtacaacgcg ccattgacca ggcagaattg
     4321 atggcagatt gtcagatctc ttcggtatat ctggcgcttt ctggtaagca catcagctgc
     4381 cagaatgaaa ttggtatggt gcctatttct gaagaagaag tgacgcaaga agatgtggaa
     4441 aacgtcgtcc ataccgcgaa atcggtgcgt gtgcgcgatg aacatcgtgt gctgcatgtg
     4501 atcccgcaag agtatgcgat tgactatcag gaagggatca agaatccggt aggactttcg
     4561 ggcgtgcgga tgcaggcaaa agtgcacctg atcacatgtc acaacgatat ggcgaaaaac
     4621 atcgtcaaag cggttgaacg ttgtgggctg aaagttgacc aactgatatt tgccggactg
     4681 gcatcaagtt attcggtatt gacggaagat gaacgtgaac tgggtgtctg cgtcgtcgat
     4741 atcggtggtg gtacaatgga tatcgccgtt tataccggtg gggcattgcg ccacactaag
     4801 gtaattcctt atgctggcaa tgtcgtgacc agtgatatcg cttacgcctt tggcacgccg
     4861 ccaagcgacg ccgaagcgat taaagttcgc cacggttgtg cgctgggttc catcgttgga
     4921 aaagatgaga gcgtggaagt gccgagcgta ggcggtcgtc cgccacggag tctgcaacgt
     4981 cagaccctgg cagaggtgat cgagccgcgc tataccgagc tgctcaacct ggtcaacgaa
     5041 gagatattgc agttgcagga aaagcttcgc caacaagggg ttaaacatca cctggcggca
     5101 ggcattgtat taaccggtgg cgcagcgcag atcgaaggtc ttgcagcctg tgctcagcgc
     5161 gtgtttcata cgcaagtgcg tatcggcgcg ccgctgaaca ttaccggttt aacggattat
     5221 gctcaggagc cgtattattc gacggcggtg ggattgcttc actatgggaa agagtcacat
     5281 cttaacggtg aagctgaagt agaaaaacgt gttacagcat cagttggctc gtggatcaag
     5341 cgactcaata gttggctgcg aaaagagttt taatttttat gaggccgacg atgattacgg
     5401 cctcaggcga caggcacaaa tcggagagaa actatgtttg aaccaatgga acttaccaat
     5461 gacgcggtga ttaaagtcat cggcgtcggc ggcggcggcg gtaatgctgt tgaacacatg
     5521 gtgcgcgagc gcattgaagg tgttgaattc ttcgcggtaa ataccgatgc acaagcgctg
     5581 cgtaaaacag cggttggaca gacgattcaa atcggtagcg gtatcaccaa aggactgggc
     5641 gctggcgcta atccagaagt tggccgcaat gcggctgatg aggatcgcga tgcattgcgt
     5701 gcggcgctgg aaggtgcaga catggtcttt attgctgcgg gtatgggtgg tggtaccggt
     5761 acaggtgcag caccagtcgt cgctgaagtg gcaaaagatt tgggtatcct gaccgttgct
     5821 gtcgtcacta agcctttcaa ctttgaaggc aagaagcgta tggcattcgc ggagcagggg
     5881 atcactgaac tgtccaagca tgtggactct ctgatcacta tcccgaacga caaactgctg
     5941 aaagttctgg gccgcggtat ctccctgctg gatgcgtttg gcgcagcgaa cgatgtactg
     6001 aaaggcgctg tgcaaggtat cgctgaactg attactcgtc cgggtttgat gaacgtggac
     6061 tttgcagacg tacgcaccgt aatgtctgag atgggctacg caatgatggg ttctggcgtg
     6121 gcgagcggtg aagaccgtgc ggaagaagct gctgaaatgg ctatctcttc tccgctgctg
     6181 gaagatatcg acctgtctgg cgcgcgcggc gtgctggtta acatcacggc gggcttcgac
     6241 ctgcgtctgg atgagttcga aacggtaggt aacaccatcc gtgcatttgc ttccgacaac
     6301 gcgactgtgg ttatcggtac ttctcttgac ccggatatga atgacgagct gcgcgtaacc
     6361 gttgttgcga caggtatcgg catggacaaa cgtcctgaaa tcactctggt gaccaataag
     6421 caggttcagc agccagtgat ggatcgctac cagcagcatg ggatggctcc gctgacccag
     6481 gagcagaagc cggttgctaa agtcgtgaat gacaatgcgc cgcaaactgc gaaagagccg
     6541 gattatctgg atatcccagc attcctgcgt aagcaagctg attaagaatt gactggaatt
     6601 tgggtttcga ggctctttgt gctaaactgg cccgccgaat gtatagtaca cttcggttgg
     6661 ataggtaatt tggcgagata atacgatgat caaacaaagg acacttaaac gtatcgttca
     6721 ggcgacgggt gtcggtttac ataccggcaa gaaagtcacc ctgacgttac gccctgcgcc
     6781 ggccaacacc ggggtcatct atcgtcgcac cgacttgaat ccaccggtag atttcccggc
     6841 cgatgccaaa tctgtgcgtg ataccatgct ctgtacgtgt ctggtcaacg agcatgatgt
     6901 acggatttca accgtagagc acctcaatgc tgctctcgcg ggcttgggca tcgataacat
     6961 tgttatcgaa gttaacgcgc cggaaatccc gatcatggac ggcagcgccg ctccgtttgt
     7021 atacctgctg cttgacgccg gtatcgacga gttgaactgc gccaaaaaat ttgttcgcat
     7081 caaagagact gttcgtgtcg aagatggcga taagtgggct gaatttaagc cgtacaatgg
     7141 tttttcgctg gatttcacca tcgattttaa ccatccggct attgattcca gcaaccagcg
     7201 ctatgcgatg aacttctccg ctgatgcgtt tatgcgccag atcagccgtg cgcgtacgtt
     7261 cggtttcatg cgtgatatcg aatatctgca gtcccgtggt ttgtgcctgg gcggcagctt
     7321 cgattgtgcc atcgttgttg acgattatcg cgtactgaac gaagacggcc tgcgttttga
     7381 agacgaattt gtgcgtcaca aaatgctcga tgcgatcggt gacttgttca tgtgtggtca
     7441 caatattatt ggtgcattta ccgcttataa atccggtcat gcactgaata acaaactgct
     7501 gcaggctgtc ctggcgaaac aggaagcctg ggaatatgtg accttccagg acgacgcaga
     7561 actgccgtt
//
LOCUS       DAFATA010000150         7538 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFATA010000150 DAFATA010000000
VERSION     DAFATA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.151"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(93..916)
                     /locus_tag="LGJ20_004381"
                     /pseudo
     CDS             complement(93..916)
                     /locus_tag="LGJ20_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(970..2688)
                     /gene="proS"
                     /locus_tag="LGJ20_004382"
     CDS             complement(970..2688)
                     /gene="proS"
                     /locus_tag="LGJ20_004382"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK2388375.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2800..3507)
                     /gene="tsaA"
                     /locus_tag="LGJ20_004383"
     CDS             complement(2800..3507)
                     /gene="tsaA"
                     /locus_tag="LGJ20_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBK2388376.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3504..3908)
                     /gene="rcsF"
                     /locus_tag="LGJ20_004384"
     CDS             complement(3504..3908)
                     /gene="rcsF"
                     /locus_tag="LGJ20_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBK2388377.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVESVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4026..4841)
                     /gene="metQ"
                     /locus_tag="LGJ20_004385"
     CDS             complement(4026..4841)
                     /gene="metQ"
                     /locus_tag="LGJ20_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBK2388378.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4881..5534)
                     /gene="metI"
                     /locus_tag="LGJ20_004386"
     CDS             complement(4881..5534)
                     /gene="metI"
                     /locus_tag="LGJ20_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBK2388379.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5527..6558)
                     /gene="metN"
                     /locus_tag="LGJ20_004387"
     CDS             complement(5527..6558)
                     /gene="metN"
                     /locus_tag="LGJ20_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBK2388380.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6746..7318
                     /gene="gmhB"
                     /locus_tag="LGJ20_004388"
     CDS             6746..7318
                     /gene="gmhB"
                     /locus_tag="LGJ20_004388"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBK2388381.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1858 a         1949 c         1911 g         1820 t
ORIGIN      
        1 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
       61 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
      121 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
      181 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
      241 tgcgccagtg taagtggatg cccggctttg gcaaaggcat ccgtgacaaa ttccgaacaa
      301 aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc caggccgctg
      361 acgcactttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
      421 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
      481 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
      541 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
      601 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
      661 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
      721 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
      781 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
      841 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaaacagg cggcagtacg
      901 cctttggttt atccatttta tacaatccat gtaaaaaaag ggccctgaaa ttcaggaccc
      961 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
     1021 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
     1081 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
     1141 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
     1201 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
     1261 cacctggaac ggcgcgatag cgtcaggcca tacgatgcct cgttcgtcgt agttctgctc
     1321 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
     1381 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
     1441 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
     1501 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
     1561 atcccagttg atgccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
     1621 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
     1681 acccggaccg gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
     1741 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
     1801 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
     1861 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
     1921 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
     1981 tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
     2041 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
     2101 ttgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
     2161 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
     2221 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
     2281 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
     2341 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
     2401 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcagc
     2461 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
     2521 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
     2581 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
     2641 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
     2701 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaaagt gatttagttt
     2761 accagtgcaa aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa
     2821 gacttcaaaa cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta gccagacggc
     2881 ataggttttg cccgtttcct cacctttacg ataggccggg cgtgggtcct gcgccagtac
     2941 ttcgcggata aacagcgtta actgcggata acgcttctcc agcgtcaaaa gctgcttttc
     3001 gacctctgcg gtaaaactca ccgccatctc tgccgctggc gcactttgcg catagctggc
     3061 actggcatcg ggaagcgatt cggcaaaggg gagatacggt ttgatatcca ctaccggcgt
     3121 accatcgacc agatccagac tgccgagctt cagaatcacg ctgtctttat ggcaaacaac
     3181 ctctttcagc tctaccagcg acataccaat tgggttaggg cggaaagtag agcgtgttgc
     3241 gaaaaccccc attctggcgt taccgccgag gcgcggtgga cgcacagtcg gacgccagcc
     3301 gccttccatc gtttgatgaa agacgaaaag gatccataaa tggctgaacg cttccaggcc
     3361 gcgaacggcg tcggcctggt tgtagggagc aatgagatgc agttcgccgt tggcgctttt
     3421 taccagacct ggctggcgcg gaacggcgaa tttttcttta tagggcgagc gaataacgcc
     3481 tatttgctcg aactggaaac tgctcatttc gccgtaatgt taagcgcaga gccgatacat
     3541 acagcctgac gatagcagcc tggcgtaccg ctggtgactt cgcagctatg cagtaatacc
     3601 gcattggcct tcattttcga ggcgttgatt tgcatccgct tacgcgcggt tggaatgctc
     3661 ggcggagagt cctgattaga ggcctggcaa gagtcgccac tgacttcacc gagatcgcgg
     3721 aacggtttgc cgactaattc ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc
     3781 gctttcggtt ttgcaggctc cgctttcggc tggggtgcag tgctttgaac ggattcgaca
     3841 ggggatctgc ttaacatgga acagccgctt agcatgagtg ctactaaaca gatcggtaaa
     3901 gcacgcatag tatttcctca atgtatgatc aaaacgtcaa tattgaatca ggagcttgta
     3961 aaaatgacaa gacgggcaag cgcccgtcct gaatgatatt acaaattgtg gaaacagcct
     4021 aaaaattacc agcctttaac agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact
     4081 tcatcagact gataagcctg gacgaatttc ttcacgttct cggcgtcttt gttatcttca
     4141 cgcgttacga tcaggtttac gtacggggag tctttatctt cgacaaagat accgtctttc
     4201 gctggagtca ggccaatctg gctggcatag gtggtattga taactgccag agcgatttgc
     4261 gcgtcgtcca gagatcgagg cagctgcggt gcttccagct caacaatttt cagatttttt
     4321 gggttctcaa caacatcaag aacggtcggc agcaggccaa cgccatcttt cagtttgatc
     4381 aagcccactt tttgcagcag cagcagtgaa cgaccaagat tagttggatc gtttggcacg
     4441 gcaacctgcg aaccatcctg cagttcatcc agtgatttga ttttcttgga gtaaccagca
     4501 atcggataaa caaaagtgtt gcctactgcg accagtttgt agccacgatc tttcagttgc
     4561 tgatcaaggt acggtttatg ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct
     4621 tcgtttggca gaacatagtc gttgaaggtt accagctcaa cgtccaggcc atatttgtct
     4681 ttcgcaactt tctgcgcgac ttctgctacc tgctgttcgg caccaacaat cacgccgact
     4741 ttaatgtggt ttggatcttt ttcatcctga ccgcagccta ccagtgccag tgatccaatc
     4801 agggctccca ctgccgcaaa ggttttgaat ttgaacgcca taccttattc cttcttcaat
     4861 tatttatgtt gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg
     4921 aactgaatta aataaaccag aatgaccagc aataccagta ccgtattcat caccgtcgcg
     4981 ttatagccga tgtagccata ctgatagcca atctgaccta aaccaccggc accgactgca
     5041 ccacccatcg cggaataacc gaccagggta atcagggtga tagttgccgc attcaccaga
     5101 cccggcaatg cttccggtaa cagcacttta cggacgatct gcatcggcgt ggcacccatt
     5161 gcacgggaag cttcaattaa cccggttggg atctccagca gagcgttctc gaccatacgg
     5221 gcaataaacg gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa accaatcgat
     5281 gtaccgacaa taacgcgggt aaacggaatc atccatacca gcaagataat gaacgggatg
     5341 gaacggaaaa tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt agcaataatt
     5401 tgccccgggc gcgtaacata aagcaggacg ccgaccggca gaccaatcac aaagccaaaa
     5461 aatccggata cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag caaccacatc
     5521 atcggctcag acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat
     5581 ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac
     5641 gccaccggcg taatccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc
     5701 ggtttcagaa agcagcgggg catcgaccga ttgaccggta aactccaggc gcaacatcgg
     5761 cacgcagtca gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag
     5821 atgcagggtc gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc
     5881 acttaccgtg tcctgctcga tcagttcgcc attgctgatg accgccacgc aatcacaaat
     5941 acgcttcaca acgtccattt cgtgggtgat caacagaatc gtcaaaccca gacggcggtt
     6001 aatgtctttc agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc
     6061 ttcatcacac agcaatactt tgggattgct ggctaatgca cgggcaatcg ccacacgttg
     6121 tttctgccca ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa
     6181 tgacagcaat tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag
     6241 cggcagagcc acgttgccaa aaacagtacg cgaagagagc aggttaaaat gctggaaaat
     6301 cataccaatc tggcggcgag ctttggtcaa ctcggattct gacagcgtgg tcagttcctg
     6361 gccatcgacc agcacgctac cctcggttgg gcgctccagc aggtttacac aacgtataag
     6421 cgtactctta cccgcgcctg aggcaccgat aacgccataa atttgtccag ctggcacatg
     6481 caggctgacg ttgttcaacg cctggatggt gcgggtgccc tggtggaaca ctttggtgat
     6541 attcgaaagt tttatcattg attatttatt atcgtcatta agttagtcgt ggcatttcga
     6601 atgcctgaaa cgggcaacgc cgtcaatgaa atggatgtta aggcatccag acgtctaaat
     6661 caatcaggtt tatgcgaaga gcactttctt gcaggtcgaa acatgcgata ctagcgtcac
     6721 atgccttatt aaggagctat aaaaggtggc gaagagcgta cccgcaattt ttcttgaccg
     6781 tgatggcacc attaatgtcg atcacggcta tgtccatgag atcgacaact ttgaatttat
     6841 cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg ggctttgcgc tggtggtagt
     6901 aaccaaccag tctggcattg ctcgcggtaa atttactgaa gcgcagtttg aaacgctgac
     6961 cgagtggatg gactggtcgc tggcggaccg cgatgtcgat ctggatggca tctattattg
     7021 cccgcatcat ccgcagggta gtgttgaaga gtttcgccag gtctgcgact gccgcaaacc
     7081 acatccgggg atgcttttgt cagcacgcga ttatttgcat attgatatgg ccgcttctta
     7141 tatggtgggc gataaattag aagatatgca ggcagcggct gcggcgaacg tgggaacaaa
     7201 agtgctggtg cgtacgggta aacctattac gcctgaagca gaaaacgcgg cggattgggt
     7261 gttaaatagc ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac cggcataatg
     7321 attaaaagat gagcaattga aataaaaatg catttttcca cttgtcttcc tgagacgact
     7381 ccctataatg cgcctccatc gacacggtgg atgtgaatca cttcacacaa acagccggtt
     7441 cggttgaaga gaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata
     7501 cgccacctcg cgacagtgcg ctaaagcgcg tcgcaact
//
LOCUS       DAFATA010000151         7520 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFATA010000151 DAFATA010000000
VERSION     DAFATA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.152"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..512)
                     /locus_tag="LGJ20_004389"
     CDS             complement(<1..512)
                     /locus_tag="LGJ20_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBK2388382.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFI"
     gene            653..1012
                     /locus_tag="LGJ20_004390"
     CDS             653..1012
                     /locus_tag="LGJ20_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBK2388383.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(1015..1284)
                     /locus_tag="LGJ20_004391"
     CDS             complement(1015..1284)
                     /locus_tag="LGJ20_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBK2388384.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(1274..1870)
                     /gene="rsmD"
                     /locus_tag="LGJ20_004392"
     CDS             complement(1274..1870)
                     /gene="rsmD"
                     /locus_tag="LGJ20_004392"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBK2388385.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            2020..3516
                     /gene="ftsY"
                     /locus_tag="LGJ20_004393"
     CDS             2020..3516
                     /gene="ftsY"
                     /locus_tag="LGJ20_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK2388386.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            3519..4187
                     /gene="ftsE"
                     /locus_tag="LGJ20_004394"
     CDS             3519..4187
                     /gene="ftsE"
                     /locus_tag="LGJ20_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK2388387.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            4180..5238
                     /gene="ftsX"
                     /locus_tag="LGJ20_004395"
     CDS             4180..5238
                     /gene="ftsX"
                     /locus_tag="LGJ20_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBK2388388.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            5483..6337
                     /gene="rpoH"
                     /locus_tag="LGJ20_004396"
     CDS             5483..6337
                     /gene="rpoH"
                     /locus_tag="LGJ20_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBK2388389.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            6608..>7520
                     /gene="livJ"
                     /locus_tag="LGJ20_004397"
     CDS             6608..>7520
                     /gene="livJ"
                     /locus_tag="LGJ20_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBK2388390.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTY"
BASE COUNT         1931 a         1848 c         2104 g         1637 t
ORIGIN      
        1 ctgataaaca gcaacgaagc gccgacaaac gcagagagcg ccggagcggt cggacggaag
       61 aaccacagtt cacctgccag ccacaccatc accagcgtca tgccgataaa cgtgcagata
      121 ggccaacgat actcttccag ccgcgtccag ataatcgcca gtaacagcgc gcccagaacc
      181 agcagcacca gtggcagagg ccagaagaaa gagagcgtca tctgactggc gaaatagatg
      241 gtgtacagca ggtgcgagag gaaaaacgcg ccgatggcgt acatcagacg ttgacgtggc
      301 aacagggtta gcgcatcgcc cagcagtgag gcgcacagcc ccgccagcac cagatagcta
      361 atggcgtcga acatcggcgc ttgccaggcc agtaacagca ggagaagaag ggttaatggt
      421 ttaaataccc agcgttgcca ggttggccca cgatacgatg catccacaga tagccatgcg
      481 gaaagacaga cagcgataaa cgaccaaagc atcttagttc cttgaattcg ttattttttc
      541 gtaagcggaa tgcctacggt cttgcttcca gtttagtggc gagggtggac ggatgacaac
      601 accataactg gcagggtatg cttaattccg cattttccga tgagggataa agatgagcaa
      661 accacctctt ttctttattg ttatcattgg cttaatcatc gtcgccgcat cgtttcgttt
      721 tatgcaacaa cgacgggaaa aagctgataa tgatatggca ccactccagc aaaagctggt
      781 gttggtgagc aacaagcggg aaaaaccgat taacgatcgc cgttcgcgcc agcaggaagt
      841 gactccggca ggcaccagta tgcgctatga ggcaagcttc aaaccgcaaa gcggaggaat
      901 ggagcagacg tttcgcctcg acgcccagca gtaccacgcc ctgacagtgg gcgataaagg
      961 tacgctgagc tataaaggaa cgcgctttgt cagctttgta ggcgaacaat aaggttattt
     1021 tttaacttta aatttcttct gccagaccag cagttcaaac acgccgaaaa ggaaaatccg
     1081 taccttttcg gcggtggtca tctgcgggcc atctttcggt aaagtggatt tcaatagcgc
     1141 cagctgcata ccatgcatca gcacggtaaa aatcagcgcc acgttaacga aaatattcag
     1201 cggacgtggg aagggatgca ccaggttgag gattaaaaat ccccaaacac agagcattaa
     1261 caaacgacca atattaatca gcatcacttt ctccttgtgc ttcgcgttga tacagccgat
     1321 aagccacctg acccgccact ttttcccgat gtaatgacca gtttgccgga acagtgggca
     1381 gaccgttttc gacttcgctt tcgacataaa tcagggcttc gtcagccagc cagccgttat
     1441 cttccagtaa atttatcgtc tcttctaaca agccacggcg gaacggtgga tcgacaaaca
     1501 cgatattatg cggtgtgcct ttttgcgcca ggaatgacat tgcgttgctg ttcaccacgc
     1561 gtgcattgcc tgcttttagt gtcgccagat tcttaattaa ctgctgagaa accgcgcgat
     1621 ccatctcaat caacgtggcc cccgcagcat agcgcgacaa cgcttccagc cccagcgcgc
     1681 cgctcccggc gaagcaatcc agacattggg cgtcaacaat gaccggagcc agccagttaa
     1741 acaacgtttc gcgtacgcgg tcggtggtgg ggcgcagacc tgggctatca ggaaccggga
     1801 gtttacggcc tcgccactgc ccgccaataa tgcggatttg gccgctgccg gaatgattcg
     1861 gttttttcat gataaattgc tcaatccgcc agataacaca tacttgcagg cggtgatgtg
     1921 aattaagtta agtgatagac tatttcatca tttttttagc tgctatgtac atagcgttaa
     1981 cgctgtgcca tgaagcaaca gcgaggagtg tagtcgcaaa tggcgaaaga aaaaaaacgt
     2041 ggcttttttt cctggctggg ctttggtcaa aaagagcaga ccccggaaaa agagacagaa
     2101 gttcagaatg aacaaacggt tgtagaagaa atcgttcagg cgcaagagcc tgtgaaggct
     2161 tctgaacaag ccgtcgaaga gcagccgcag gcgcatactg aggccgaggc ggaaactttt
     2221 gctgccgacg ttgtggaagt cactgaacag gttgctgaaa gtgaaaaagc gcagcctgaa
     2281 gcggaagtcg ttgcacaacc ggaaccggtc gtagaagaaa cgccggagcc agtggctatc
     2341 gaacgtgaag agctgccgtt gccggaagac gtcaacgccg aagaggtttc gccagaagag
     2401 tggcaggctg aagcggaaac cgtagagatt gtcgaagcgg cggaagaaga agcggctaaa
     2461 gaagaaatca ccgacgaaga gctggaagca caggcgctgg ctgccgaagc ggcagaagaa
     2521 gcggtgatag tggttcctcc ggcagaagaa gagcagccgg ttgaagaaat tgcccaggag
     2581 caggaaaaac cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct gttaaaaacc
     2641 aaagaaaatc tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat cgacgatgat
     2701 ctgtttgaag agctggaaga acagctgttg atcgccgatg tgggcgtgga aaccacacgt
     2761 aaaatcatca ccaatctgac ggaaggcgca tcccgcaagc agcttcgtga tgccgaggcc
     2821 ctctatggcc tgctgaaaga agagatgggc gagattctgg cgaaagtcga tgagccgctg
     2881 aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtag gcgtcaacgg tgtgggtaaa
     2941 accacgacga ttggtaagct ggcgcgtcag tttgagcagc agggtaaatc ggtgatgctg
     3001 gcggcgggcg atactttccg tgcagcagcg gttgaacagc ttcaggtctg ggggcagcgc
     3061 aacaatattc cggtgattgc ccagcatacc ggtgcggatt ccgcctctgt tatcttcgac
     3121 gccattcagg ccgctaaagc gcgtaatatc gacgtcctga ttgccgatac cgccggacgc
     3181 ctgcagaaca aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt gatgaagaaa
     3241 ctcgacgttg aagcgccgca tgaagttatg ctgactatcg atgccagcac cgggcagaac
     3301 gcggtaagcc aggccaaact gttccatgaa gctgttggct taaccggcat cacgctaacg
     3361 aaactggacg gcacggcgaa aggcggggta attttctcgg tggctgacca gtttggtatc
     3421 cctatccgct acattggtgt cggcgaacgt attgaggatt tgcgtccgtt taaggcggac
     3481 gactttatag aggcactttt tgcccgagag gattaacaat gattcgcttt gaacatgtca
     3541 gcaaggctta tctcggtggg agacaggcgc tgcagggcgt tacgttccat atgcagccgg
     3601 gtgagatggc gtttctgacc ggtcattccg gcgcagggaa aagtaccctc ctgaagctga
     3661 tctgtgggat tgagcggccc agcgccggga aaatctggtt tagcggccat gacatcacgc
     3721 gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat tggcatgatt ttccaggatc
     3781 accatctact gatggaccgt actgtctacg ataacgtggc aatcccgctg attatcgccg
     3841 gtgccagcgg tgacgatatt cgtcgccggg tgtcggcggc actggataaa gtcgggctac
     3901 tggacaaagc gaagaacttc cctattcagc tttctggcgg tgaacaacag cgtgttggca
     3961 ttgcccgcgc ggtggtgaac aagcccgcgg tactgctggc ggacgaaccg actggtaacc
     4021 tggacgacgc gttgtcggaa gggattctac gtctgtttga agagtttaac cgcgttgggg
     4081 taaccgtatt gatggcaacg cacgacatca acctgatctc gcggcgttcc tatcgcatgc
     4141 tcaccctgag cgatggtcac ttgcatggag gcgtgggcca tgaataagcg cgatgcaatc
     4201 aatcatattc ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt cggcggctct
     4261 ggcgacggcg gtcgtaacgc gccaaaacgc gcgaaatcct cgccaaaacc ggtaaatcgc
     4321 aaaaccaacg ttttcaacga acaggtgcgc tatgccttcc acggcgcatt gcaggatctg
     4381 aaaagcaaac cgttcgccac gtttttaacg gtgatggtta tcgccatctc cctgacgctg
     4441 cccagcgtct gttatatggt gtacaaaaac gttaaccagg cggcgacgca atattatccg
     4501 tcaccgcaaa tcactgttta cttgcaaaaa acgctggacg atgacgccgc agcaggcgtg
     4561 gtagcacagc tacaggccga gcagggcgtg gagaaagtga actatctttc tcgtgaagac
     4621 gcactgggcg agttccgtaa ctggtctggt tttggtggtg cgctggatat gctggaagaa
     4681 aacccgcttc cggcagtggc ggtggtgatc ccgaaactcg attttcaggg gacggaatca
     4741 ctgaatacgc tgcgtgatcg tatcacccag attaacggca ttgacgaagt gcggatggat
     4801 gacagctggt ttgcccgtct ggcggcgttg accgggctgg tcgggcgcgt ttccgcgatg
     4861 atcggcgtgt tgatggtggc agcagtgttc ctcgtcatcg gcaacagtgt gcgtctgagc
     4921 atctttgctc gccgtgactc cattaacgtg cagaaactga ttggtgcgac ggatggtttc
     4981 atcctgcgcc cgttcctgta cggaggcgca ttgctgggat tttctggcgc attgctgtca
     5041 ttaattttgt cagaaattct ggtactgcgt ttgtcatcgg cggttgcaga agtggcacag
     5101 gttttcggaa cgaagtttga tatcaatggc ttatcgttcg atgaatgcct gctgttgctg
     5161 ctggtttgct ctatgattgg ctgggtggca gcgtggcttg ccacggtaca acatttacgc
     5221 cactttacgc ctgaataata aaagcgtgtt atactctttc cctgcaatgg gttccgtagc
     5281 agggaaagag accccgttgt ctcttcccgg tatttcatct ctatgtcaca ttttgtgcgt
     5341 aatttattca caagcttgca ttgaacttgt ggataaaatc acggtctgat aaaacagtga
     5401 atgataacct cgttgctctt aagctctggc acagttgttg ctaccactga agcgccagaa
     5461 gatatcgatt gagaggattt gaatgactga caaaatgcaa agtttagctt tagccccagt
     5521 tggcaacctg gattcctaca tccgggcagc taacgcgtgg ccgatgttgt cggctgacga
     5581 ggagcgggcg ctggctgaaa agctgcatta ccatggcgat ctggaagcag ctaaaacgct
     5641 gatcctgtct cacctgcggt ttgttgttca tattgctcgt aattatgcgg gctatggcct
     5701 gccacaggcg gatttgattc aggaaggtaa catcggcctg atgaaagcag tgcgccgttt
     5761 caacccggaa gtgggtgtgc gcctggtctc cttcgccgtc cactggatca aagcagagat
     5821 ccacgaatac gttctgcgta actggcgtat cgtcaaagtt gcgactacca aagcgcagcg
     5881 caaactgttc ttcaacctgc gtaaaactaa gcagcgtctg ggctggttta accaggatga
     5941 agtcgaaatg gtggcccgtg aactgggcgt aaccagcaaa gacgttcgtg agatggaatc
     6001 acgtatggcg gcacaggaca tgacctttga cctgtcttcc gacgacgatt ccgacagcca
     6061 gccgatggca ccggtgctct atctgcagga taaatcatct aactttgccg acggcatcga
     6121 agatgataac tgggaagagc aggcggcaaa ccgtctgacc gacgcgatgc agggtctgga
     6181 cgaacgcagc caggacatca tccgtgcgcg ctggctggac gaagacaaca agtccacgtt
     6241 gcaggaactg gctgaccgtt acggtgtttc cgccgagcgt gtgcgccagc tggaaaagaa
     6301 cgcgatgaaa aaacttcgcg ctgccattga agcgtaattt ctactactaa gcagagaacc
     6361 ctggatgaga atccggggtt tttgtttttt gggcctctac aataatcaat tccccctccg
     6421 gcaaaacgtc aatccccacg cagattgtta ataaactgtc aaaatagcta ttccaatatc
     6481 ataaaaatgg ggtatgtttt agcagagtat gctgctaaag cacgggtagt catgcataaa
     6541 acgaaataaa gtgctgaatc acaacaccac aacacacgta ataaccagaa gaatggggat
     6601 tctcaggatg aacataaagg gtaaagcgtt actggcagga tgtatcgcgc tggcattcag
     6661 caatatggct ctggcagaag atattaaagt cgcggtcgtg ggcgcaatgt ccggtccggt
     6721 tgcgcagtac ggtgaccagg agtttaccgg cgcagagcag gcggttgcgg atatcaacgc
     6781 taaaggcggc attaaaggca acaaactgca aatcgtaaaa tatgacgatg cctgtgaccc
     6841 gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac ggcattaaat atgtgattgg
     6901 tcacctctgt tcttcatcaa cgcagcctgc gtctgacatc tacgaagacg aaggcatttt
     6961 aatgatcacc ccagcggcaa ccgcgccgga gctgaccgcc cgtggctatc agctgatcct
     7021 gcgcaccacc ggcctggact ccgaccaggg gccgacggcg gcgaaatata ttcttgagaa
     7081 agtgaaaccg cagcgtattg ctatcgttca cgacaaacag caatacggcg aaggtctggc
     7141 gcgagcggtg caggacggcc tgaagaaagg caatgctaac gtggtgttct ttgatggtat
     7201 caccgccggg gaaaaagatt tctcaacgct ggtggcgcgt ctgaaaaaag agaatatcga
     7261 cttcgtttac tacggcggtt atcacccgga aatggggcaa atcctgcgtc aggcacgcgc
     7321 ggcagggctg aaaactcagt ttatggggcc ggaaggtgtg gctaacgttt cgctgtctaa
     7381 cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact acgatcaggt
     7441 tccggcgaac aaacccattg ttgacgccat caaagcgaaa aaacaggatc caagtggcgc
     7501 gttcgtctgg accacctacg
//
LOCUS       DAFATA010000152         7473 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFATA010000152 DAFATA010000000
VERSION     DAFATA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.153"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1092
                     /locus_tag="LGJ20_004398"
     CDS             <1..1092
                     /locus_tag="LGJ20_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK2388391.1"
                     /translation="FTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYN
                     PEPETARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARD
                     GVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVR
                     GGGELGQQWYEEGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVA
                     INQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYD
                     NVTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSG
                     RFKSYEGVAMEYAFLVALAQGTLPATPAD"
     gene            complement(1089..1751)
                     /gene="exoX"
                     /locus_tag="LGJ20_004399"
     CDS             complement(1089..1751)
                     /gene="exoX"
                     /locus_tag="LGJ20_004399"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBK2388392.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1775..2431)
                     /locus_tag="LGJ20_004400"
     CDS             complement(1775..2431)
                     /locus_tag="LGJ20_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBK2388393.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(2533..2763)
                     /gene="holE"
                     /locus_tag="LGJ20_004401"
     CDS             complement(2533..2763)
                     /gene="holE"
                     /locus_tag="LGJ20_004401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBK2388394.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2902..3276
                     /gene="yobA"
                     /locus_tag="LGJ20_004402"
     CDS             2902..3276
                     /gene="yobA"
                     /locus_tag="LGJ20_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBK2388395.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGSKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3280..4152
                     /gene="copD"
                     /locus_tag="LGJ20_004403"
     CDS             3280..4152
                     /gene="copD"
                     /locus_tag="LGJ20_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBK2388396.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            4165..4506
                     /locus_tag="LGJ20_004404"
     CDS             4165..4506
                     /locus_tag="LGJ20_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBK2388397.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            4902..5558
                     /gene="pphA"
                     /locus_tag="LGJ20_004405"
     CDS             4902..5558
                     /gene="pphA"
                     /locus_tag="LGJ20_004405"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBK2388398.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFEPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(5559..5750)
                     /locus_tag="LGJ20_004406"
     CDS             complement(5559..5750)
                     /locus_tag="LGJ20_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBK2388399.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(5855..6091)
                     /locus_tag="LGJ20_004407"
     CDS             complement(5855..6091)
                     /locus_tag="LGJ20_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBK2388400.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(6209..>7473)
                     /gene="rsmF"
                     /locus_tag="LGJ20_004408"
     CDS             complement(6209..>7473)
                     /gene="rsmF"
                     /locus_tag="LGJ20_004408"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBK2388401.1"
                     /translation="YGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEAS
                     SMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHAN
                     ISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQ
                     EIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGDL
                     FPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKDR
                     EAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNK
                     GYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIG
                     LAKRIGSRLKNSYPRELVRDGKLFTGNA"
BASE COUNT         1867 a         1806 c         1888 g         1912 t
ORIGIN      
        1 tttaccgact ggctggtggt tgaagagcgt cagcgcgggt taaccagttt gcgccaaatt
       61 aaccgcaaga cccgggaagt cattggtatt gcctttgatg atccggccta tgtgacctgg
      121 attgcctaca atccagaacc tgaaaccgcg cgattgcgtt atggttattc ttccatgacc
      181 acaccagata ctttgtttga actggatatg gataccggtg agcgtcgtgt attaaaacaa
      241 acggaagttc ctggttttga tgcggcgaat taccgcagtg aacacctgtg gatagtcgcc
      301 cgtgatggcg tcgaagttcc ggtttcgttg gtctaccatc gcaaacattt tcgcaaagga
      361 cacaacccgt tgctggtgta tggctatggt tcttacggcg caagtattga tgccgatttc
      421 agttttagcc gcttgagttt gttagatcgt ggctttgtct acgccattgt ccatgttcgc
      481 ggcggtggtg agctggggca acaatggtac gaagagggaa aatttctgaa aaagaaaaat
      541 acgtttaatg attatcttga tgcctgcgat gcattgttaa aactgggcta tggctctcct
      601 tcgctttgtt atgcgatggg cgggagtgcg gggggcatgt tgatgggcgt tgcaattaat
      661 caacgcccgg aattattcca cggcgttatc gcccaggtac cgtttgttga tgttgtaaca
      721 acgatgcttg atgaatcaat tcctcttacc actggtgagt ttgaagagtg ggggaatccg
      781 caggatccgc aatattacga gtacatgaaa agctacagcc catatgacaa cgtcaccgca
      841 caggcttatc cgcatttact ggtaacgacc ggtttgcacg attctcaggt gcaatattgg
      901 gaaccggcaa aatgggtcgc taaattgcgc gagctgaaaa ccgatgacca tcttttattg
      961 ctctgtaccg acatggactc aggccatggc ggtaaatctg gtcgctttaa atcgtacgaa
     1021 ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc agggaacatt acccgctacg
     1081 cctgcggact aagtattttc cagataatgt ttcagtgtta aacgcagctc cgggctcatg
     1141 ctgtccaggt tattaaataa ccagcgcaga tagcccggat cgcgttcggc aacgtcggaa
     1201 accgctttgc cacggtattt gccaaaggtg aaggtcgtca tcaacgacgg acgtccggtg
     1261 atatcggcca tctgttctgc cgtccagccg gaggtgttca taatatcgat aagcaacgcg
     1321 gcggtgatat aacagtcata caacgcgcgg tgatgatgca ggcccggcgg cgtctgtaca
     1381 ttgagcttgc gtgttttata taacgccata ttgctgtact tgatcccagg ccacaaacga
     1441 cgggccagtt tcatagtgca aatccactca ccgggcatct caggcagtac gcggcggtca
     1501 aagctggcgt tatgcgcgac ataccattca ctaccgtaat agtgtgggat cacatcttca
     1561 atccacggtt tatcggcgac catggcttcg gtgatgcgat gaatcgccat cgcttgtgga
     1621 ctaataggac gatcggggcg caccaggtgg ctcatggggt tgacgatttt tccgtcaatg
     1681 acatcaacag aggcaatctc aacgatccct ccctgcaaac cgcaggtttc tgtatcgata
     1741 atgcgcaaca tggcatgctc caggccgaaa aagcctagcg taatggaatg atatcgcctt
     1801 gccaaccctg tgatgaacgc tgaccgacta acaataatga accacgatcg gcgcgaacga
     1861 tgagacgacc atattcatcc catagtgcac tgttaccacg ggcatttgcc atcagcacgg
     1921 caatcgagta ctgatgtgaa aaaaattgta agcgacgggc cgacgccagc aggtcgggtt
     1981 ctcccagaca ttgccctgta gtaaacagtg aacaggtcgg gtccatatcc ataccttccg
     2041 gttgttcatc gaccaccgtg atggttctgg aacgacgacc cagacatgca ccatgactct
     2101 gatggtagat cccaggcgtt tttcgccacg gtgcgaacac cgcaattcca cgaataaagc
     2161 gatcgttata ttcaacggga aggccggcaa tgatggtcat tcgccaggtc gttgcggcat
     2221 aacaaagcgg gtctaacagt gagagatcgg gtggggcagg aagggcacgt cgcgaataat
     2281 cacaccccag taaagaaagc gatggaaaaa ctaacagctg acattgttgc ctggcggcgg
     2341 ccctcacaaa ctccagatga tgagctacct gttcggtgag tgaggtttta cgaggctcat
     2401 attgcgctgc tgcaactttc cagaacgaca taatgacttc cttttcaaaa tgactgcaaa
     2461 ttatgggtgc ggtttagaaa atactcataa tttagcaggc gttatgtaag aaagtgtaac
     2521 tctgtataag ttttatttaa gtttgggctc gtaaggtaaa cgtgacagat tgaccgaagc
     2581 caaacggtgg gcaataagcc gctcgcgaaa ccagctgcgc aaatgttcag gctgttcacg
     2641 ttcaaccgct tcagcgatca ccggcatatt gtagcgttct ttaaatgcca ccccggccgc
     2701 cgccaaatcg acattcactt tatccatttc tgtttgatcc agtttagcca gattcttcag
     2761 cattcttaat ctcctcatca ttcgcgggca aaagttacta agagtgagcc aggatggcaa
     2821 gccggaattg atgatcctcg actcagacat tagacaaggt tatgctctgt aaaaaagtta
     2881 ttacaaaaag gaatagataa tatgacttca actgcacgct cccttcgcta cgcgctggca
     2941 atcctgacca cttcgttggt gacaccttcc gtctgggcac atgcgcattt aacgcatcag
     3001 tatcctgcgg caaacgcgca agtgacagct gcaccgcagg caatcacttt aaacttctcg
     3061 gaaggtgttg aaacaggatt cagtggtgca aaaatcacgg ggtcaaaaaa cgaaaatatt
     3121 aaaacattac cggcgaagcg aaatgagcag gaccaaaagc aactgattgt tccactggcc
     3181 gattcgctga aacccggaac ctacatcgtt gactggcatg ttgtttcggt ggacgggcat
     3241 aaaacgaaag gacactacac ctttagcgtg aaataaatca tgctggcgtt tacctggatc
     3301 gcgctgcgat ttatccattt cacctcgctg atgctggtct ttggcttcgc aatgtacggt
     3361 gcctggctgg cccccttaac gattcgtcgc ttgctggcga agcgttttct gcgtttacaa
     3421 cagcacgctg ccgtctggag tttgatcagt gccactgcaa tgctcgccgt tcagggcggg
     3481 ttgatgggaa cgggatgggc agatgtattt tcgccaaaca tttggcaggc tgttttgcaa
     3541 acgcagtttg gtggcgtctg gttatggcaa attgttctcg ccctcgtaac gttgatcgtc
     3601 gcccttatgc aaccgcggaa tatgccacgt ttgctgttta tgctcactac cgcgcaattt
     3661 attctgctgg cgggaatcgg gcatgcgacg ctgaatgaag gggtaacggc gaaaatccat
     3721 cagactaatc acgcgattca cctgatttgt gcggcggcgt ggtttggggg attactgccg
     3781 gtgctctggt gtatgcaact catcaaaggc cgttggcgac accaggctat tcaggcgctg
     3841 atgcgttttt cctggtgcgg gcattttgcg gtgatcggcg tactggcaag cggcgtgctt
     3901 aatgcattgt tgattaccgg atttcccccc acgctaacga cttactgggg ccaactctta
     3961 ttgctcaaag ccatcctggt gatgattatg gtggtcattg cgctggctaa tcgttatgtt
     4021 cttgtaccgc gcatgaggca ggatgaagat cgtgcggcac cctggtttgt gtggatgact
     4081 aaactcgaat gggcgatagg tgcggtagtg ctggtgatca tcagcctgct tgcgactctc
     4141 gaaccttttt gatggattac gaaaatgatg aaaaaaagta ttctggcgtt tctgttactc
     4201 accagttctg cagcggcgct ggctgcaccg caggtgatta ccgtcagccg ttttgaagtg
     4261 ggtaaagaca aatgggcgtt taatcgcgaa gaggtgatgc tgacttgccg accgggtaat
     4321 gctttgtatg tcatcaaccc aagtaccctc gtgcagtatc ctttaaacga tatcgcacaa
     4381 aaggaagttg ccagtgggaa gactaaagcc caacccattt cggtgattca gattgatgat
     4441 cctaacaatc ccggcgaaaa aatgagtctg gcaccgttta tagaacgagc tgaaaaactc
     4501 tgttaattac ctaaaatagc cttttgattt ccaataaaaa aaccgcctca gttctttcac
     4561 cagaacgggc ggtttttaac atttcagctg atgaccacca cgctttttat tgaccatttt
     4621 gcacgcaaac tggaaaacct ggcgtcgtca tctattctta aagggcaagg caactaagcc
     4681 tgcattaatg ccaactttta gcgcacggct ctctcccaag agccatttcc ctggaccgaa
     4741 tacaggaatc gtgttcggtc tctttttatc tgttaaaagc cagaagcatt tccttcgctg
     4801 actttatagt caaccataac acacactcta ctgtctgagt ccagcgtttt ttaacattct
     4861 tgttaagatt atgtgatctt tagcgcggga ggaaaatatt gatgaaacag cctgcgcccg
     4921 tttatcagag aattgcgggt catcaatggc gacatatctg gctttctggc gatatacacg
     4981 gttgtcttga gcagttgcgc cgcaaattat ggcattgtcg ttttgaaccg tggcgagatt
     5041 tacttatctc agtaggagac gttatcgatc gtgggccgca aagtttacgt tgtctgcagt
     5101 tactggaaca acattgggtt tgtgcggtaa gaggcaatca tgaacagatg gcgatggatg
     5161 cgctggcatc ccagcagatg tctttgtggt tgatgaatgg cggcgactgg tttattgcgc
     5221 tggcagataa tcaacagaaa caagcgaaaa cggcgctgga aaaatgtcag catttgccct
     5281 ttattcttga agtacacagt cgtaccggca aacatgttat tgctcatgcc gattatccag
     5341 atgatgttta tgaatggcaa aaggacgttg atttgcatca ggtcttgtgg agccgctcgc
     5401 gattaggtga acgccaaaaa gggcagggaa ttacaggtgc tgatcatttc tggtttggtc
     5461 atacaccgtt gcgacatcgc gtggatattg gcaacctgca ttatattgat accggtgctg
     5521 tctttggggg cgaactgact cttgtgcaat tgcaataatt aaaaatcacc atactcctgt
     5581 gctggtcgcc agaaaccatc tataaaatcc tcaatcggaa aacaaccgcc atggcggatc
     5641 cgttgatcgc tcatagaata aagacactgc tgttccgtgt tgtagacatc cacaacaata
     5701 tcttcacaac cgccatccag gtagcaaaca aaaagtacca gcgcgaacat ttcatccccg
     5761 aagtgtggtg ccgtaccgtt aagtttagga gagattttac aacgggggaa taaccaggac
     5821 aaataacccg ccatgccgac gggttctttt tggatcaggc aagacgcata atccaggcat
     5881 cagattgttg atcgtaacgg gtacggtaaa ggacagagtg ttcgccattt agcagggcag
     5941 ggatactggt ttcagcatcc caggcatcca gttgcataca taaatcgggg tcagatttac
     6001 aaggaatggt taacgttcga tcacctggcg attcaccgtg taattcgccg tcgtcgattt
     6061 caaaagcgcc tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccgattg
     6121 tggtacgacc agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag
     6181 tcaaaaagtg cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc
     6241 gcaccagttc acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa
     6301 tcggttgatg ctggaaagta accaatacat catccgccac tggcgcggct tgcgggtaaa
     6361 catcgcgccc gcgataccac tcctccgctt cctgcggtgt cagctcgaat gcattcatat
     6421 tatcgggtga ggcaagagca ataaccgctt catgctgcca gcgataacct ttgttgtgcg
     6481 tctcggcaag tttaatcccc aaccgagaaa atcggacttt accgatcagg gcttcaatgc
     6541 ccaccgggaa caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt
     6601 ttaagccaac acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcaccgggc
     6661 tgaacgggaa attgcccact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt
     6721 tacgcagacg agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa
     6781 agccttcttc ggtcagcgct ttgtttgcac cagggaagag atcgccaagt ggtaaaaact
     6841 ctactgcgtc ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt
     6901 ttaaggtaca ggtcgagtaa accagcgtac caccaggacg taatgcatga aaggcgctgt
     6961 cgataagctc ccgttgtgta gctgcgattt cctgattgct ttctggtgac cagtttttta
     7021 gcgcatcggg atctttacgc accacgcctt cgccagagca gggagcgtcc agcaaaatgg
     7081 catcgaacat ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa
     7141 cattactgat gccacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa
     7201 actcattggc aaggattgcc ccttcgttat tcatccgcgc ggcaatttgc gtcgttttgg
     7261 agcctggcgc agctgcgaca tccatcaccc gctgtggtgc attatcgtca gcaaacaagg
     7321 cggcaacggg caacattgaa ctggcttcct gaatataaaa caggccgctt aaatgctcgg
     7381 cggtactacc caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac
     7441 accacggaat tggcgtaagc gtccagccat aag
//
LOCUS       DAFATA010000153         7324 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFATA010000153 DAFATA010000000
VERSION     DAFATA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.155"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..837)
                     /locus_tag="LGJ20_004409"
     CDS             complement(<1..837)
                     /locus_tag="LGJ20_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK2388402.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTA"
     gene            complement(910..1545)
                     /locus_tag="LGJ20_004410"
     CDS             complement(910..1545)
                     /locus_tag="LGJ20_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK2388403.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            1673..2191
                     /gene="yhbO"
                     /locus_tag="LGJ20_004411"
     CDS             1673..2191
                     /gene="yhbO"
                     /locus_tag="LGJ20_004411"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBK2388404.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(2171..2614)
                     /locus_tag="LGJ20_004412"
     CDS             complement(2171..2614)
                     /locus_tag="LGJ20_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBK2388405.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            2665..2967
                     /gene="yhbQ"
                     /locus_tag="LGJ20_004413"
     CDS             2665..2967
                     /gene="yhbQ"
                     /locus_tag="LGJ20_004413"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBK2388406.1"
                     /translation="MTPWYLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(2954..3457)
                     /locus_tag="LGJ20_004414"
     CDS             complement(2954..3457)
                     /locus_tag="LGJ20_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2388407.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(3451..3975)
                     /locus_tag="LGJ20_004415"
     CDS             complement(3451..3975)
                     /locus_tag="LGJ20_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBK2388408.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPIKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            4184..5179
                     /locus_tag="LGJ20_004416"
     CDS             4184..5179
                     /locus_tag="LGJ20_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK2388409.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            5188..6066
                     /locus_tag="LGJ20_004417"
     CDS             5188..6066
                     /locus_tag="LGJ20_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK2388410.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            6272..7279
                     /locus_tag="LGJ20_004418"
     CDS             6272..7279
                     /locus_tag="LGJ20_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBK2388411.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         1732 a         2062 c         1895 g         1635 t
ORIGIN      
        1 tgccgtggga atgacaaaca agcatcctgc taccgccatc gccatcaccc acatcaggct
       61 gttatcgaca gcaccatcgg catgggggaa taaccagacg cgagcggcac ccaacaccag
      121 tactgcaagg atgtaaaccg ggatcgtact ccagaaaagc gtccatagcg ccctgcccca
      181 gcggctaaaa aaaccgccct gtgcttccgg tatgtcaatt tcgaccggtg cctgcgtttg
      241 cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc agcaacacca tcaccagccc
      301 ggccaccaga cgaatcgccg caaaacccca gccgaggaca aaccccataa acaccagcgt
      361 cgccgggttt aacaccggat tgcccatcca gaatgccagc gcaccgccca tcgacacctg
      421 ttggcgacgc attcccgccg cgaccggagc cgcacagcag gtacacatca tgcccggcag
      481 cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat tgcccaagcg tacgcaacaa
      541 ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg atcaccccca gcactgccgc
      601 tttccatacc gcgaggaaat agatcatcgc gtaatccaac gccgcctgcc acggattagc
      661 atccgcctgc gcaaggatag atttaccgat actgtgggtt tcggcagcag taaacgcttt
      721 gccgtagtaa ggttgccatt tcacatacca gagaccggca atgacaacga gaaagaaaag
      781 cgcgggtttc caccactgaa tgggcgttgc cgcctgagat gaagactgac cagtcatagc
      841 attccccagg aataagttat gataattgag cgcgtgaata ttacgctcac tatcaattct
      901 tgggaataat tattcagctc ttttgcgtaa ttctgaagag cttaaaatcg tcacaccttc
      961 atgcccgggc cgcatcgcct ctgccagcat gacccgcgcc acgtctctcg catcaatgga
     1021 tttccagtta ccgggtaata aacggaacag cggcgcaaaa agcgtttcgt tcatccgctg
     1081 tttgctacga tcgcccagta acatcgacgg gcgagcaatg gtcaatttcg gccagttctg
     1141 ggcgattaat gcttcttcca tctccccttt gacgcggtta tagaaaaacg gcgagtgggc
     1201 attggcaccc atagcactga ccaccagcat atgctgcgcg cccagtcgcc gcccggttaa
     1261 tgcggtatcc actaccagcg tgtaatcggc atgaataaac gcctctttgc tccccgcttc
     1321 tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg ggatcggtga cctgcgccag
     1381 cgcgtcactc agttgcggat catggggatt aaacaccccg ggcatatcgc ccaacggacg
     1441 tcgcgtcggc gcagcaatgg cgttaacttt cggttcgtta atcaacatcc gcagcaggtg
     1501 accgcccacc agccccgttg cgcctgtaat cagtacctga ctcatcttcg ctcctttaca
     1561 gaattgtctg ccttgcgctc cacggctcat gcaccaggct taatagacca tgaggtaatt
     1621 atccccgatt gtgggaaatt cgcctcatcc aatgcaacaa cgcggaggaa gcatgagtaa
     1681 gaaaattgcc gttttaatca ctgatgaatt tgaggattca gaatttactt cacccgcaga
     1741 cgagttccgt aaagccggac acgaagtgat taccattgaa aaacaagcgg gtaaaacggt
     1801 gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa tccatcgatg aagtgacgcc
     1861 tgcggagttt gatgccctgc tgctaccggg cggccattca ccggattatc tgcgtgggga
     1921 caaccgtttt gtcaccttta cccgtgattt tgtgaatagt ggcaaaccgg tgtttgccat
     1981 ctgtcacggc ccgcagttgc tgatcagcgc cgatgtgatt cgggggcgca agctgaccgc
     2041 agttaaaccg atcattattg atgttaaaaa tgcgggcgcg gaattttacg atcaggaagt
     2101 tgtggttgat aaagatcagc tggttaccag ccggacaccg gacgatctgc cagcgtttaa
     2161 ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag ccaaatcatt tttttaccaa
     2221 agcccagcgt gttgtcggtg aatttgattt catcgagccg gatttcccat accggggccg
     2281 acagcattct ggcaaccgga aagcgacggt tgtacgcctt gcgtgcgagg tcgctttcct
     2341 caccttccag cctgcggatc tcacctttaa actgcacacc gcgaattaac gctactgttt
     2401 tcggctgacc gtttaccgtt ccggcaactg ccgcctgcgg cccactcatc tgcgcatgac
     2461 gtgttttttc ttccgtcaaa atgtagaagg cgactttctg tgcatcaaaa agataaaagg
     2521 cattggcgca ccagagttcc ccttcctgct gcacacacca ggtgacaaca tgttgttttg
     2581 ccagccaacg actgatggcg gtgagtgttt ccattgctgt tctcttttat actgttggcc
     2641 tgaccttaac atgtgacgca gacgatgaca ccctggtatc tttacttgat ccgtaccgcc
     2701 gacaataagc tttataccgg gatcaccacg gatgtcgaac gccgctatca gcaacatcaa
     2761 agcggcaaag gggcgaaagc actgcgcggg aaaggagaac tgacgctggc gttttctgcg
     2821 ccagtgggcg atcgttcgct ggcgttacgg gcagaatatc gcgttaagca actgaccaaa
     2881 cggcaaaaag agcgtctggt agcggaaggc gcagggtttg cagagctgtt gagcagcctg
     2941 caaaccccaa agattaaaag cgattgaaat gctcgtgata ctcaaccagg ccggtaacgc
     3001 cattcagcgc gtcatccgct aaacgatgta cctggaaggc gctttcagtg cccggccagc
     3061 ggcaacgcag atcgtgatgc gccgccagtt caaagccgaa acgactgtac agcgccggat
     3121 cgcccagcgt caccactgcg gcatagccga actcattaag cgaatcgagt ccttcataga
     3181 ccagttggcg tgccagcccc tgtccacggt atttttcatc aaccgccaac ggtgccatgc
     3241 cgacccattg caggtcttcg ccctgcacat caaccggact aaatgccaca tagccaatga
     3301 cctgaccttc gtcatctgtc gccaccagcc ccagcgtcag aaagccatct tcacgcagat
     3361 cgtgaaccag cttcgcttcc gcatcacttt cgaatgagcg acgcagcagg gcatcaatac
     3421 ccggcgcatc aatgggaatt tctactcgaa ttagcatggt tcacctaccg atgtctgttt
     3481 ggtttcaggc gcggttttca ttcccgcctc aacaaaatcc gccagttgca gcagcatcat
     3541 gcgtaaggct ttcggcattt gctccagttc aatggcgtcc atcaggtttt tcacatacag
     3601 ccccagctcc gtatcgcctt caatcaccag ccgacgctgg aagaagagcg tatccggatc
     3661 ttgtttacgc gccgcaatca tcagcagatc gctggcgtcg gcactaaaac tcacatcagc
     3721 ttgcgcgttc tggctgacca ccagtttgcc attcaccacc gaggtaaacc attgcaggtc
     3781 aatatcacgc acatgaatac ttaaccagcg gccttcaaga aactccagct caccatcatc
     3841 cagcgcctga cggaattgcc agcttaagac ctgctcaaga acctggcgtt ttagcgcaaa
     3901 tggcgtcagt tttatcggta cactcaacag agatggcccc aaatgcacaa tacgggaacg
     3961 cagtttatcc aacacgagtt ttactccctg tttcaacaat catcctattt tgccatatca
     4021 gaaaaataac atagcggtat aaatcaacaa ttccatatga aattgctgct accaccaata
     4081 caactttaac tgccttaaat caaaaattgt cgcagcaagg ttaactaaaa tcccagttcg
     4141 ttaacatttt tgcgttttga tagcgcaacc ttcaggaaaa attatggagc tgctctgccc
     4201 tgccggaaat ctcccggcgc ttaaggcggc catcgaaaac ggcgcagatg ctgtttatat
     4261 cgggctaaaa gatgatacca atgcccgtca cttcgccggc cttaacttta ccgagaaaaa
     4321 attgcaggaa gcggtgagtt ttgtccatca acatcgccgc aaacttcaca tcgcgattaa
     4381 cacttttgcg catccggacg gttacgcccg ttggcagcgc gccgtggata tggcggcgca
     4441 gctgggtgcc gacgcgctga tcctcgccga cctcgccatg ctggaatacg ccgccgagcg
     4501 ttatccgcat attgagcgcc acgtatcggt gcaggcttcg gcgaccaatg aagaggcgat
     4561 taacttttat catcgccatt ttgacgttgc ccgcgtggtg ctgccgcgcg tgttgtcgat
     4621 tcatcaggtg aaacagctgg cacgggtcac acctgtacca ctggaagtgt ttgctttcgg
     4681 cagcctgtgc attatgtcgg aaggccgttg ctatctgtcg tcgtatctga cgggtgagtc
     4741 gcctaacacc gtgggcgcgt gttctccggc ccgtttcgtg cgctggcaac aaacgccgca
     4801 ggggctggaa tcccgcctga acgaagtgct gatcgaccgt tatcaggacg gcgaaaacgc
     4861 aggttatccg acgctgtgta aagggcgtta tctggtggac ggcgagcgct atcacgcgct
     4921 ggaagaacca accagtctca ataccctgga actgctgccg gagttaatgg cggcgaatat
     4981 tgcttcggtg aaaattgaag gccgccagcg tagcccggcg tatgtcagcc aggtggcgaa
     5041 agtctggcgt caggctatcg accgttgtaa ggccgatccg caaaacttca taccgcaaag
     5101 cgcgtggatg gagacgctcg ggtcgatgtc cgaaggcacg cagaccaccc ttggcgcgta
     5161 tcaccgtaaa tggcagtgag aagagcaatg aaatattcct tagggccagt gctgtggtac
     5221 tggccaaaag agacgctaga agatttttat cagcaggccg ccaccagcag cgccgacgtg
     5281 atttatcttg gtgaagcggt atgcagcaag cgccgggcaa ccaaagttgg cgactggctg
     5341 gagatggcaa aatcgctcgc cggaagtggc aagcagattg tgctctccac gctggcgctg
     5401 gtgcaggcat cctctgaact gggcgaactg aaacgctatg ttgagaacgg tgagtttctg
     5461 attgaagcca gcgatctcgg cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc
     5521 gccgggcacg cgctgaactg ctacaacgcg gtgacgctga aaatattgct caaacagggc
     5581 atgatgcgct ggtgtatgcc ggtggagctt tcccgcgact ggctggtgaa tctgcttaat
     5641 cagtgcgatg agctgggcat tcgcaaccag tttgaagtgg aagtcctgag ctacggtcat
     5701 ctaccgctgg cctactccgc ccgctgcttt accgcgcgtt cggaagaccg cccgaaagat
     5761 gagtgtgaaa cctgctgcat taagtatccg aacgggcgca acgtgctgtc gcaggaaaac
     5821 caacaagtgt ttgtactcaa tggcattcag accatgagcg gctacgttta caacctcggt
     5881 aacgagctgg catccatgca ggggctggtt gatgtggtcc gcctgtcacc gcagggtact
     5941 gacactttcg cgatgctcga cgccttccgc gctaatgaaa atggcgctgc gccactaccg
     6001 ttgacggcaa acagcgactg taacggctac tggcggcggc tggccgggct ggagctgcaa
     6061 gcctaagtaa atatgctcgt catacttcaa gttgcatgtg ctgcatctgc attcgttcac
     6121 cccagtcact tacttatgta agctcctggg gattcactct cttgtcgcct tcctgcaact
     6181 cgaattattt agagcacagc tcactttgtt aacaacttta actactcttt aatgcagtat
     6241 taaagattaa tcggtaacaa agtgagctgt tatgactgat aaaaccattg cgttttcgct
     6301 actcgatctg gcccccattc ccgaaggttc ttcagcgcgg gaagcattct cccactctct
     6361 cgatctcgcc cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cagaacacca
     6421 caatatgact ggcattgcca gtgctgccac gtcggtattg attggctatc tggcggcgaa
     6481 taccaccacg ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt
     6541 cattgccgaa cagttcggca cgctcaatac actctatccg gggcgaatcg atttggggct
     6601 gggtcgtgcg ccaggtagtg accaacggac aatgatggcg ctgcgtcgcc atatgagcgg
     6661 cgatattgat aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga
     6721 tcccaatccg catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgtt
     6781 aggctccagc ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt tcgcgtttgc
     6841 ctcacacttc gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa
     6901 accgtcggca cgactggaaa aaccatacgc gatggtgtgc atcaatatta tcgccgccga
     6961 tagcaaccgc gacgctgagt ttctgtttac ctcaatgcag caagcctttg tgaagttgcg
     7021 ccgtggcgaa accgggcaac tgccgccacc gattcaaaat atggatcagt tctggtcgcc
     7081 gtccgaacag tatggtgtgc agcaggcgct gagtatgtcg ttggtaggtg ataaagcgaa
     7141 agtgcgtcat ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggtcaa
     7201 cgggcagatt ttcgaccacc aggcgcggct gcattcgttt gagctggcga tggatgttaa
     7261 ggaagagttg ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct
     7321 gtcc
//
LOCUS       DAFATA010000154         6984 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFATA010000154 DAFATA010000000
VERSION     DAFATA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.156"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..405)
                     /locus_tag="LGJ20_004419"
     CDS             complement(<1..405)
                     /locus_tag="LGJ20_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK2388412.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVP"
     gene            769..3555
                     /gene="polA"
                     /locus_tag="LGJ20_004420"
     CDS             769..3555
                     /gene="polA"
                     /locus_tag="LGJ20_004420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK2388413.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(3937..4569)
                     /locus_tag="LGJ20_004421"
     CDS             complement(3937..4569)
                     /locus_tag="LGJ20_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK2388414.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            5151..5660
                     /gene="yihI"
                     /locus_tag="LGJ20_004422"
     CDS             5151..5660
                     /gene="yihI"
                     /locus_tag="LGJ20_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBK2388415.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            5849..>6984
                     /gene="hemN"
                     /locus_tag="LGJ20_004423"
     CDS             5849..>6984
                     /gene="hemN"
                     /locus_tag="LGJ20_004423"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK2388416.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLI"
BASE COUNT         1913 a         1680 c         1803 g         1588 t
ORIGIN      
        1 tggcacccag gccagctgtt gcttgaggaa atatttattc atcggaatgt gcttacgaaa
       61 cagcacgcac agtacgacaa tatctgccca actacggtgg ttacaaataa gcagatacca
      121 gttcttctta cttaaccctt ccagcccgtg aacttcccat tgcaggtgtg ggttcagatg
      181 cagtaatacc gccagacctt cacaccagca atacatcata aaatcacaaa aacgcgaaac
      241 ctttcgccaa atgactggca caggtagcaa tagttttaca atcccggcaa tgatgatcgg
      301 gacggaacaa aaaatagtga ccaaaatggt caatacgata ctcagcaaaa gggttatcgc
      361 agcgagtatt ctcgtcataa tgaatttatt caaaaggtta gccatagaca gtgcgctaaa
      421 gaagcgcaag gcgcagattt tagcagaaaa cgacccaaat aacggatgat ccttaaggag
      481 aaaaataatt catatctatc cacattagaa aaaatcccat tatctcaatt attagggatg
      541 gctttatttt taactgcatg aaaaacaaag acaaacatca tgctgtaaaa agcatgataa
      601 taaattaaaa gcgatgtaaa taatttatgc acaaagttat ccacatgacg atttgcgagc
      661 gatccagaag atctacaaaa gattttcacg aaaagcggtg aaaaactcat gttttcatcc
      721 tgtctgtggc atcctttacc cataatctga taaacaggca cggacattat ggttcagatc
      781 ccccaaaatc cacttatcct tgtagatggt tcatcttatc tttatcgcgc atatcacgcg
      841 tttcccccgc tgactaacag cgcaggcgag ccgaccggtg cgatgtatgg tgtcctcaac
      901 atgctgcgca gtctgatcat gcaatataaa ccgacgcatg cagcggtggt ctttgacgcc
      961 aagggaaaaa cctttcgtga tgaactgttt gaacattaca aatcacatcg cccgccaatg
     1021 ccggacgatc tgcgagcaca aatcgaaccc ctgcacgcga tggttaaagc gatgggactg
     1081 ccgctgctgg cggtttctgg cgtagaagcg gacgacgtta tcggtactct ggcgcgcgaa
     1141 gccgaaaaag ccgggcgtcc ggtgctgatc agcactggcg ataaagatat ggcgcagctg
     1201 gtgacgccaa atattacgct tatcaacacc atgacgaata ccatcctcgg accggaagag
     1261 gtggtgaata agtacggcgt gccgccagaa ctgatcatcg atttcctggc gctgatgggt
     1321 gactcctccg ataacattcc tggcgtaccg ggcgtcggtg aaaaaaccgc gcaggcattg
     1381 ctgcaaggtc ttggcgggct ggatacgctg tatgccgagc cagaaaaaat tgctgggttg
     1441 agcttccgtg gcgcgaaaac aatggcagcg aagctcgagc aaaacaaaga agttgcttat
     1501 ctctcatacc agctggcgac gattaaaacc gacgttgaac tggagctgac ctgtgaacaa
     1561 ctggaagtgc agcaaccggc agcggaagag ttgttggggc tgttcaaaaa gtatgagttc
     1621 aaacgctgga ctgctgatgt cgaagcgggc aaatggttac aggccaaagg ggcaaaacca
     1681 gccgcgaagc cgcaggaaac cagtgttgca gacgaagcgc cagaagtgac ggcaacggtg
     1741 atttcttatg acaactacgt caccatcctt gatgaagaaa cactgaaagc gtggattgcg
     1801 aagctggaaa aagcgccggt atttgcattt gataccgaaa ccgacagcct tgataacatc
     1861 tctgctaacc tggtcgggct ttcttttgct atcgagccag gcgtagcggc atatattccg
     1921 gttgctcatg attatcttga tgcgcccgat caaatctctc gcgagcgtgc actcgagttg
     1981 ctaaaaccgc tgctggaaga tgaaaaggcg ctgaaggtcg ggcaaaacct gaaatacgat
     2041 cgcggtattc tggcgaatta cggcattgag ctgcgtggga ttgcgtttga taccatgctg
     2101 gagtcctaca ttctcaatag cgttgccggg cgtcacgata tggacagcct cgcggaacgt
     2161 tggttgaagc acaaaaccat cacttttgaa gagattgcgg gtaaaggcaa aaatcaactg
     2221 acctttaacc agattgccct cgaagaggcc ggacgttacg ccgccgaaga tgctgatgtc
     2281 accttgcagt tgcatctgaa aatgtggccg gacctgcaaa aacacaaagg gccgttgaac
     2341 gtcttcgaga atatcgaaat gccgctggtg ccggtgcttt cacgcattga acgtaacggt
     2401 gtgaagatcg atccgaaagt gctgcacaat cattctgaag agctcacctt gcgtctggct
     2461 gagctggaaa agaaagcgca tgaaattgca ggtgaagaat ttaacctttc ttccaccaag
     2521 cagttgcaaa ccattctgtt tgaaaaacag ggtattaaac cgctgaagaa aacgccgggt
     2581 ggcgcgccgt caacgtcgga agaggtactg gaagaactgg cgctggacta tccgttgcca
     2641 aaagtgattc tggagtatcg tggtctggcg aagctgaaat cgacctacac cgacaagctg
     2701 ccgctgatga tcaacccgaa aaccgggcgt gtgcatacct cttatcacca ggcagtaact
     2761 gcaacgggac gtttatcgtc aaccgatcct aacctgcaaa acattccggt gcgtaacgaa
     2821 gaaggtcgtc gtatccgcca ggcgtttatt gcgccagagg attatgtgat tgtctcagcg
     2881 gactactcgc agattgaact gcgcattatg gcgcatctct cgcgtgacaa aggcttgctg
     2941 accgcattcg cggaaggaaa agatatccac cgggcaacgg cggcagaagt gtttggtttg
     3001 ccactggaaa ccgtcaccag cgagcaacgc cgtagcgcga aagcgatcaa ctttggtctg
     3061 atttatggca tgagtgcttt cggtctggcg cggcaattga acattccacg taaagaagcg
     3121 cagaagtaca tggaccttta cttcgaacgc taccctggcg tgctggagta tatggaacgc
     3181 acccgtgctc aggcgaaaga gcagggctac gttgaaacgc tggacggacg ccgtctgtat
     3241 ctgccggata tcaaatccag caatggtgct cgtcgtgcag cggctgaacg tgcagccatt
     3301 aacgcgccaa tgcagggaac cgccgccgac attatcaaac gggcgatgat tgccgttgat
     3361 gcgtggttac aggctgagca accgcgtgta cgtatgatca tgcaggtaca cgatgaactg
     3421 gtatttgaag ttcataaaga tgatgttgat gccgtcgcga agcagattca tcaactgatg
     3481 gaaaactgta cccgtctgga tgtgccgttg ctggtggaag tggggagtgg cgaaaactgg
     3541 gatcaggcgc actaagattc gcctgaacat gccctttttt cgtaagtaag caacataagc
     3601 tgtcacgttt tgtgatggct attagaaatt cctatgcaac aactgaaaaa aaattacaaa
     3661 aagtgctttc tgaactgaac aaaaaagagt aaagttagtc gcgtagggta cagaggtaag
     3721 atgttctatc tttcagacct tttacttcac gtaatcggat ttggctgaat attttagccg
     3781 ccccagtcag taatgactgg ggcgtttttt attgggcgaa agaaaagatc cgtaatgcct
     3841 gatgcgctat gtttatcagg ccaacggtag aattgtaatc tattgaattt acgggccgga
     3901 tacgccacat ccggcacaag cattaaggca agaaaattat tcgccgtcct gcgtttcttc
     3961 tacaggctgc atctcgctaa accaggtatc cagtttctgc cgcagcttgt ccacgccttg
     4021 tttcttcaac gaagaaaacg tttcaacctg cacatcaccg ttaaacgcca gtacagcttc
     4081 acgcaccata ttcaattgcg ctttacgtgc gccgcttgcc agtttgtccg ctttggtcag
     4141 cagcaccaga acggcgatat tgctgtctac cgcccactca atcatctgct gatccaaatc
     4201 tttcagcgga tggcgaatat ccattagcac caccagacct tgcaggctct gacgtttttc
     4261 gaggtattcg ccgagcgcac gctgccattt gcgcttcatc tcttccggga cttccgcata
     4321 accgtaccca ggcaagtcaa ccagacgctt gccgtcagcc acttcaaaca ggttgataag
     4381 ctgggtgcgc cctggggttt ttgaggtacg agccaggctt ttctggttag tcagcgtgtt
     4441 cagcgcgctg gatttacctg cgttggaacg gcctgcaaaa gccacttcaa ttccggtatc
     4501 ggaaggtagg tggcgaatat caggcgcact catcacaaaa tgcgtctgtt gataattcaa
     4561 attagtcaaa gcggtcgtct ccgtcagtca aagctttgtg gcgattatac ctgaaccata
     4621 ataaaaggcg gatttttcgg cgtgagcgtt gtaagtaaaa gccatacgct ttgtgagaca
     4681 ttgccgatag tctttatgcg aaatagcaga gaaaattctg cgatgcatgt caaataagct
     4741 atataaatca gtgaattgct tttatataaa actctgaaaa tgtgcaaata ctgatggcgg
     4801 ttgattgttt gtttaaagca aaggcgtaaa gtagcaccca tagagcgagg acgctaacag
     4861 gaacaatgac tcaggatgag ggtcaggagc gccaggaggc gaagacagag gattgtcagg
     4921 aagacaaacg tccggagacg taattaaacg gaaatggaat caacacggat tgttccggct
     4981 aaaggaaaaa cagggtgtgt tggcggcctg caaggattgt aagacccgtt aagggttatg
     5041 agtcaggaaa aaaggcgaca gagtaatctg tcgccttttt tctttgcttg ctttctgtta
     5101 gattccgccg caaatctata ctgaataaaa cggctaaaag acgaaccatt atgaaaccat
     5161 catcttcaaa ctcacgcagc aaaggtcatg caaaagcgcg tcgaaaaaca cgcgaggagc
     5221 tggatcagga agctcgtgat cgcaagcgtc agaaaaaacg tcgtggtcat gcgccgggca
     5281 gccgcgcagc gggcggaaac accacgtcag gcagcaaagg ccagaacgca ccaaaagatc
     5341 cacgtattgg cagtaaaacc cctattccat tgggcgtgac tgaaaaagtc accaaacagc
     5401 acaaaccgaa gagtgagaaa cctatgcttt caccgcaggc ggagttggaa ttactggaaa
     5461 cggatgagcg tctggatgcg ctgctggaac gtctggaagc aggcgaaacc ctgagtgccg
     5521 aagagcaatc ctgggtggat gctaaactgg atcgtattga tgagttgatg cagaaactcg
     5581 gcctctctta tgacgatgac gaagaagagg aagaagacga gaagcaagaa gacatgatgc
     5641 gtctgctgcg gggcaactaa cggattgccg ccgtgggcct tcccgttctg ctgataaccc
     5701 ttccggttat atgttatctg gtgtggttat tcgttaaact acagcggttg tcgcggcgac
     5761 aaaagtggct gcgcaaccgg ctgatgaccc gaaacgggca tccgccggta cgccgtagcc
     5821 gccagagacg ccatcggaag gagtgagcat gtctgtacag caaatcgact gggatctggc
     5881 cctgatccag aaatataact attccgggcc acgatacacc tcgtacccga ccgcgctgga
     5941 gttttcagaa gacttcggcg aacaggcgtt tttacaagcc gtggcgcgct atcctgagcg
     6001 tccattatct ctctacgtac atatcccgtt ctgccataag ctttgttact tctgcggttg
     6061 caataagatt gttactcgcc agcagcacaa ggccgatcag tatctggacg cgctggagca
     6121 agaaatcgtc catcgtgcac cgctgttcgc cgggcgtcac gtcagccaat tgcactgggg
     6181 cggcggaacg ccgacgtatc tgaataaagc gcaaatcagc cgcctgatga agctgctgcg
     6241 cgaaaacttc cagttcaatg ccgatgcgga gatttcgatc gaagtcgatc cgcgggaaat
     6301 cgaactggat gtactcgatc atttacgcgc cgaaggcttt aatcgcctga gcatgggcgt
     6361 gcaggacttc aacaaagaag tgcaacgtct ggttaaccgc gagcaggatg aagagttcat
     6421 ctttgcactg cttaaccatg cgcgtgagat tggttttacc tccaccaaca tcgacctgat
     6481 ttacggcctg ccgaaacaga cgccggagag tttcgccttt accctgaaac gtgtggcgga
     6541 gctgaacccc gatcgtctga gtgtctttaa ctacgcgcat ctgccgacca tttttgctgc
     6601 tcagcgcaaa atcaaagatg ctgacctgcc gagtccgcag caaaaactcg atatcctgca
     6661 ggaaaccatc gccttcctga cgcaatcggg ctatcagttt atcggtatgg atcactttgc
     6721 ccgtccggat gacgagctgg cggtggccca gcgtgaaggc gtgctgcatc gtaacttcca
     6781 gggctacacc actcagggcg ataccgatct gctggggatg ggcgtttccg ccatcagcat
     6841 gattggcgac tgctacgcgc agaaccagaa agagttgaag cagtactatc agcaagtgga
     6901 tgaacaaggc aatgcgctgt ggcgtggtat tgcgctaacg cgtgatgact gtattcgccg
     6961 cgatgtgatt aagtcgctca tctg
//
LOCUS       DAFATA010000155         6909 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFATA010000155 DAFATA010000000
VERSION     DAFATA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.157"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..572)
                     /locus_tag="LGJ20_004424"
     CDS             complement(<1..572)
                     /locus_tag="LGJ20_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HBK2388417.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQA"
     gene            complement(575..1246)
                     /gene="cecR"
                     /locus_tag="LGJ20_004425"
     CDS             complement(575..1246)
                     /gene="cecR"
                     /locus_tag="LGJ20_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBK2388418.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAECLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            1475..2839
                     /gene="rhlE"
                     /locus_tag="LGJ20_004426"
     CDS             1475..2839
                     /gene="rhlE"
                     /locus_tag="LGJ20_004426"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBK2388419.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(3071..3553)
                     /gene="ybiA"
                     /locus_tag="LGJ20_004427"
     CDS             complement(3071..3553)
                     /gene="ybiA"
                     /locus_tag="LGJ20_004427"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBK2388420.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            3673..5823
                     /gene="dinG"
                     /locus_tag="LGJ20_004428"
     CDS             3673..5823
                     /gene="dinG"
                     /locus_tag="LGJ20_004428"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBK2388421.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            5851..6813
                     /gene="ybiB"
                     /locus_tag="LGJ20_004429"
     CDS             5851..6813
                     /gene="ybiB"
                     /locus_tag="LGJ20_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBK2388422.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
BASE COUNT         1584 a         1819 c         1913 g         1593 t
ORIGIN      
        1 tgcgcctgtt cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg
       61 tactgacgca atttatcctg tgctgatttc agcgttgcct gcgcctggtc gcgcgaggag
      121 cgggcatttt ccaggtcatt tgccgaaata gtgcggcttt tccacaaccc ttgctggcgg
      181 ttatagaagt tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc agcggcggcc
      241 tgagcgattt cttcatcgcg atacccggca agcatcaggt catactgcgc ctgtgccacc
      301 gagacgcccg ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc
      361 agcacctggc ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc
      421 ccaacacgga aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg
      481 ttatcctggc ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact
      541 accgccagtc cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt
      601 gcaaaatcag gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat
      661 cgaacgcggt ccagccggta cgtaacaaaa tggtttcttt gccaagacgg aacgccagaa
      721 tctcaccaat caacgcatgg gtgtgaagga tcatgcgggt atcgttggcg tcgcagccgg
      781 tccaggcggc aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca
      841 ccaggtggta ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga
      901 cggtgtcatc ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac
      961 ggatagctgc gcgatcaggc tgtggttgtg cgaacaagca ttcggcttct tcggcgtgcg
     1021 gacgaaactg ctcgccaatg aaatcggcaa tccattgggc gcaggcgagg tacaaatctt
     1081 ctttcgaacc gaagtaatag gtgatggcag cgatattctg cccggcctgg gcggctatct
     1141 cgcgggtggt ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct
     1201 gttttttcgc ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc
     1261 ttaatcaaat gattgattaa gattatgact ccatagggga gttgtccagt atggctaaga
     1321 attttggcta cgccagtcac agggataatt tatgcgctgt gtcacaaaaa ctgctacact
     1381 ccgctccctc atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact
     1441 acaccggtaa cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag
     1501 ccctgatatc ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca
     1561 gcaggcgatc cctgcggtgc tggaaggccg cgacctgatg gctagcgccc agaccggtac
     1621 cggtaaaaca gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca
     1681 cgccaaaggg cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggctgc
     1741 gcagattggc gaaaacgtcc gtgattacag caaatatttg aacattcgtt cgctggtggt
     1801 gtttggcggt gtcagcatta acccgcaaat gatgaagctg cgcggcggcg ttgatgttct
     1861 ggtggcaaca ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca
     1921 ggttgaaatc ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga
     1981 tatccgtcgc gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac
     2041 cttctctgac gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga
     2101 agtggcacgc cgcaataccg cgtctgatca ggtgacacag cacgttcact ttgtcgataa
     2161 gaaacgtaaa cgggaactgt tgtcgcacat gattggcaaa gggaactggc agcaggtgct
     2221 ggtgtttacc cgtaccaaac atggcgctaa ccatctggct gaacagctca ataaagatgg
     2281 catccgcagt gcggcgatcc acggtaataa atcgcaaggt gcgcgtaccc gtgcgctggc
     2341 cgattttaaa tcgggtgaca ttcgcgtact ggtggcaact gacatcgctg cgcgcggtct
     2401 ggatatcgaa gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta
     2461 tgtccaccgt atcgggcgta ccggtcgtgc cgcagccacc ggagaagcgt tgtcgttggt
     2521 gtgtgttgat gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc
     2581 gcgcattgcg attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa
     2641 cggtcgccag caacgtggcg gcggcggtcg tgggcaaggt ggtggtcgcg gtcaacagca
     2701 accacgccgt ggggaaggtg gcgcaaaatc tgcaagcgcg aaacctgcag aaaaaccgtc
     2761 tcgccgcctc ggcgatgcca aaccggcagg cgaacaacaa cgtcgccgcc gtccgcgtaa
     2821 acctaccgct gcgcagtaat cttttatgcc gggctatgcc cggcatcagg ctgatgaaca
     2881 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
     2941 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
     3001 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
     3061 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
     3121 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
     3181 caaccagttt tgcgggcgcg gttgccagca agagcgcacg cagttctgga tgctgttcga
     3241 atttagcgcg aagtgctttt cgcatcacct gttctttgac cgactcccag tttttacgca
     3301 gaggcttaga acgatcgcgc cccatgcgtg cggcgaccat aggggaagaa acccgacgga
     3361 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tctgaggtgg
     3421 gccaggtttt cccgtcaacc ttgatgggcc aggcggcaaa gttagaaaaa tcaccgtagt
     3481 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
     3541 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
     3601 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
     3661 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
     3721 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
     3781 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
     3841 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
     3901 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
     3961 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt tgggcgtggg
     4021 cgctacgttt gcccgcgtaa tctgacggcg ctcgccagca ctgagcccac gcaacaggat
     4081 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
     4141 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatactgat
     4201 atcgctatag atgacgatct ctggcgtcgt ttaagtaccg acaaagccag ctgcctcaac
     4261 cgcaactgtt actactatcg tgaatgcccg ttttttgttg ctcgtcggga gattcaggaa
     4321 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
     4381 ctacctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
     4441 gcccgggatg cgctggagat gagtgccgaa atcaccgcgc cctggtatcg actacagctg
     4501 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
     4561 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
     4621 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
     4681 gcgatgggcg aactgcctga tgaagtgctg gagatctgcc agcggctggc aaaactcacc
     4741 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
     4801 cacgatattg tgcgtctgca tcggttgatt ttgcagatga accgcgcgtt ggggatgttc
     4861 gaggcgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
     4921 accaaatggg cgacgcggga agagcgcgaa gggcagctgc atctctggtt tcactgcgtg
     4981 gggatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
     5041 gtcacctccg caaccttgcg ttcgctgaac agtttttcgc gtttgcagga gatgagcggg
     5101 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
     5161 cagggcaaaa ttgttattcc ccggatgcgc gttgagcctt ccatcgacaa cgaagagcag
     5221 catattgccg aaatggcggc ctttttccgt gagcaggtgg agagcaaaaa acatctcggt
     5281 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
     5341 ttacgcctga tgctgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
     5401 cgcaaacgcg ttgccaacgg tgagcatagt gtgctggtgg gcttacagtc atttgccgaa
     5461 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
     5521 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
     5581 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
     5641 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgacc
     5701 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
     5761 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
     5821 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
     5881 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgcc
     5941 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctacgt
     6001 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
     6061 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
     6121 gcgcgtaaac aggccaacct gacgccgttg ttggcgattc tcctgcacaa actcggtttt
     6181 cctgtggtgg ttcacggggt tagcgaagat ccaacccgcg tgctgactga aaccattttt
     6241 gaattgatgg gtattacgcc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
     6301 catcaaccgg tgtttatgcc agtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
     6361 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
     6421 tttgccgaag gtgaggcgct gcgtctttcc agcgtttcgc atccggaata cattggacgc
     6481 gtcgcgaagt tctttagcga tatcggcggg cgggcgctac taatgcatgg tactgaaggt
     6541 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
     6601 cgggtgctgt atgaaaaaca ggacactgct ggtagcgagt tactgccaca agcaaaagat
     6661 ccggaaacca cggcgcagtg gattgagcgt tgccttgctg gcagcgaacc gattcccgaa
     6721 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
     6781 gacggcctgg cgcgcgttaa tcaggcattt taattctttt ctcagccgga tgagccatgt
     6841 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccacgc
     6901 ccggcgtgc
//
LOCUS       DAFATA010000156         6881 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFATA010000156 DAFATA010000000
VERSION     DAFATA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.158"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..340
                     /locus_tag="LGJ20_004430"
     CDS             <1..340
                     /locus_tag="LGJ20_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBK2388423.1"
                     /translation="PVARHTTDGLLKLKVKTNSSSSVLVGPLFGSSAPAPVTVGNTAA
                     PAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDVDKALKLLDEAERLGSTSARS
                     TFISSVKGKG"
     gene            569..2149
                     /locus_tag="LGJ20_004431"
                     /pseudo
     CDS             569..2149
                     /locus_tag="LGJ20_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            2372..2869
                     /gene="ubiC"
                     /locus_tag="LGJ20_004432"
     CDS             2372..2869
                     /gene="ubiC"
                     /locus_tag="LGJ20_004432"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBK2388424.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            2882..3754
                     /gene="ubiA"
                     /locus_tag="LGJ20_004433"
     CDS             2882..3754
                     /gene="ubiA"
                     /locus_tag="LGJ20_004433"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBK2388425.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(3909..6332)
                     /gene="plsB"
                     /locus_tag="LGJ20_004434"
     CDS             complement(3909..6332)
                     /gene="plsB"
                     /locus_tag="LGJ20_004434"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBK2388426.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            6503..6871
                     /gene="dgkA"
                     /locus_tag="LGJ20_004435"
     CDS             6503..6871
                     /gene="dgkA"
                     /locus_tag="LGJ20_004435"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK2388427.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
BASE COUNT         1742 a         1561 c         1724 g         1854 t
ORIGIN      
        1 tccggttgct cgtcatacca ccgatggctt actgaaactg aaagtgaaaa cgaactccag
       61 ctccagcgtg ttggtaggac ctttatttgg ttcttccgct ccagctccgg ttacggtagg
      121 taacacggcg gcaccagctg tggctgcacc cgctccggca ccggtgaaga aaagcgagcc
      181 gatgctcaac gacacggaaa gttattttaa taccgcgatc aaaaacgctg tcgcgaaagg
      241 tgatgttgat aaggcgttaa aactgcttga tgaagctgaa cgcctgggat cgacatctgc
      301 ccgttccacc tttatcagca gtgtaaaagg caaggggtaa ttacgcccca cagtgctgat
      361 tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta tgcttccttc ctgggatatg
      421 agcgattttt tatagtaact cacttcttct tcactaagaa tatccattat ctcaatgcct
      481 tatcagagat tcttttcctt tcgccggtag tgtctggaca ttcaggctac ttttccaggt
      541 tattttattt ctgttatgca gaggttttat gataagtcat atcctaaatt ctggcggcaa
      601 taactctttg atgaaacatg atgtggtgca aggaaataat atagtagatc ttgatttact
      661 acgtaatttc aatggggtgc caggtttaaa cagagataac tttatttata tcagcaatat
      721 tttttcaaat ataaaacaac ggaacgaaaa aatcatgcaa taaatatgtt tcgtgaagtc
      781 tcaatcagta atgatactat aagtgtaaaa ttctacagaa atgaaaaaaa ttgaatgcgc
      841 ttgcaatttt ctgatggata aagatgcgca ggggtatatc gacctgtctg atttggattt
      901 aacaagttgt cattttaaag gtgacgttat atcgaaggtg tcttttttat catcaaatct
      961 acaacatgta acattcgaat gtaaagaaat tggggattgc aattttacta ctgcaatagt
     1021 tgataatgtc atatttagat gtcgacgttt acacaatgtg atttttatca aagcgagtgg
     1081 tgaatgtgtc gatttcagca aaaatattct tgatacagtt gacttctcgc agagtcaact
     1141 tactcagagt aattttcgcg aatatcagat tagaaattca aacttcgata attgttatct
     1201 ttacgcttcg cacttcacca gagcagagtt tctgtctgcc aaagaaatat catttattaa
     1261 atcgaatatg acagctgtta tgtttgatca tgtgcgaata tcgacaggga attttaaaga
     1321 ttgcattaca gaacaattgg aattaactat tgattattca gatatatttg ggaatgaaga
     1381 tctcgatggt tatatcaata acattataaa aatgattgat acattgccag ataatgcaat
     1441 gatattgaaa tccgttctgg cagtaaaact ggttatgcaa ttaaaaattc ttaatattgt
     1501 taataaaaac tttattgaga atatgaagaa aacatttagc cattgtcctt atataaaaga
     1561 tccaattata cgcagttata tccattctgg tgaagataac aagttcgatg attttatgcg
     1621 tcaacatcga ttcagcaagg tggatttcga tacccaacag atgatccatt ttattaacag
     1681 gtttaatatg aataaagggc tgattgataa aaataacaat ttttttatcc aacttatcga
     1741 tcaggcctta cgatcaacgg atgatatgat caaagcaaat gcctggtatc tttataaaga
     1801 gtggattcgt agtgatgatg tttcacctat atttatagaa actgaagaaa agttaagaac
     1861 ctttaacact aataaattaa cacgaaacga taatatattt atcctgttct cctctgtcga
     1921 tgatgggcct gttatggtgg taagctccca gcgcttacat gatatgttga atcctacaaa
     1981 agataccaat tggaattcca cgtatatcta caaatccaga catgagatgt tgcctgttaa
     2041 tcttactcag gaaacacttt tcagctccaa atctcatggt aaatatgcgc ttttccccat
     2101 ttttactgcg agttggcgag ctcatcgtat aatgaataag ggtgtttaag taaaggaaaa
     2161 catcaccgtt cctggcatcc tgggcggtga tgcccctatg gttgccctct ccagcgcggg
     2221 catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc atgcgattgt tgcgtttttg
     2281 ttgcgtatta gatcacttaa tttgctttac atctcccgta aacacttttc tgcgatacaa
     2341 tgcctttacg ttatgtaacg gagagttcgg catgtcacac cccgcgttaa cgcaactgcg
     2401 tgcgctgcgc tattttaaag agatccctgc cctggaaccg caactgctcg actggctgtt
     2461 gctggaggat tccatgacaa aacgttttga acagcaggga aaaacggtaa gcgtgacgat
     2521 gatccgcgaa gggtttgtcg agcagaatga aatccccgaa gaactgccgc tgctgccgaa
     2581 agagtctcgt tactggttac gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc
     2641 cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt
     2701 gggtaaaacg ccgttaggac gctatctgtt cacatcatcg acattaaccc gggactttat
     2761 tgagataggc cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa
     2821 accgctgttg ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa
     2881 tatggagtgg agtctgacgc agaataagct gctggcgttt catcgcttaa tgcgtacgga
     2941 taagccaatt ggcgcgttac tgctgctctg gccaacatta tgggcgttgt gggtggcgac
     3001 accgggcgtt cctcagctct ggatcctggc ggtatttgtc gcgggtgtct ggctgatgcg
     3061 cgctgctgga tgtgtggtga atgattatgc tgaccgaaag tttgatggtc atgttaagcg
     3121 cacggcgaac cgaccacttc ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt
     3181 tgtagtgctg gtactgattt cgtttttact ggtactgacg ctgaatacga tgaccattct
     3241 gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg tttatgaagc ggtataccca
     3301 tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg cttttgccgc
     3361 tgtgagtgag tcggtgccat tgagttgctg gttaatgttc ctcgccaata ttctctgggc
     3421 ggtggcttac gacacgcagt atgcgatggt tgaccgcgat gatgatgtga agattggcat
     3481 taaatccacg gcaatcctgt tcggccaata cgataaattg attattggta ttttgcagat
     3541 tggcgtactg gcactgatgg cgatcatcgg tgagttaaat ggcttaggct ggggatatta
     3601 ctggtcaatt gtggtggctg gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg
     3661 cgagcgtgaa gcctgcttta aagcatttat gaataataac tatgttggtc tggtactgtt
     3721 tttaggactg gcaatgagtt actggcattt ctgatgatgt aaaaaagccg gatgatcatc
     3781 cggctttctt ctgggttgcc ttatgcgcgg cgcttctcag gcctacacaa cacatcgcaa
     3841 tttattgaat ttgcagatta tggaaggccg gataaggcgt tttcgccgca tccggcaatt
     3901 ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta
     3961 atcaactccg ccagcaactg ataaaccttc atcgtttctg ccggttcggc gtcgccgcta
     4021 tcgctgatat acccttcatc acgcagcgtc agcaccagag aactgaacac cgccttgtcg
     4081 aagaactccg gcgcgttgat gccgtgcagc acggagagac gttgcgcgac ggtgcggctc
     4141 tctttctcca gcgtaccgcg gttgatcgac gggttggcac tcaacaacca gaaggtgatg
     4201 gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca gctgtagggt gcgagaatgc
     4261 gccgggttga tatgcaactc atcatcttgc agggtaatca gcccctgacg ttgcatctca
     4321 tttgccagcg catcaataac gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc
     4381 gctttcagca ttgggtaaag cacattgacg tgctccatca atacgtcgcg ggagatgtgg
     4441 cgatgctggg tgacaattgc cgccatcagc gaaggcagca ccaacatatg cgcaatgttg
     4501 ttgcgatagt aggtcatcag caccgcttgc tcgcgtggca gaatgatgat gtcgccgata
     4561 gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg
     4621 ctggctgaag gaacggtaga gtctgtggag taaggtacgt tgcgcatcag atccaggtag
     4681 cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga tgccagaagc
     4741 gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga
     4801 tcggcagcaa tattattgac cgtcggcgtt aaccaggccg gacgcaccgc ttcgatggga
     4861 tcgatagatt cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa cggcattggt
     4921 tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc
     4981 tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact
     5041 tccatgacgt gctcataacc gatatagatc ggaatcagcg taatcggacg cgtgccgcca
     5101 cgcagcatcg cctgaatggt catcgacagc gtaccggttt tcggatccag caaacgcccc
     5161 gtgcgggaac gaccgccttc cacgaagtac tcgacggaat aaccacggct gaacagttcg
     5221 ccgagatact cacggaaaac ggtggaataa agtttattgc ctttaaaagt acggcgaata
     5281 aagaacgccc ccagacggcg gaaaatcggc ccggccggcc agaagttcag gttgatcccg
     5341 gcggcgatat gcggcggcac cagcccctgg tggtacagca cgtaagaaag tagcaggtag
     5401 tccatgtgac tgcggtggca aggcacatac accagctcat gaccgtcgtg ggccagctgg
     5461 cgaacgcgct cggcgttatg gacgttgatg ccctgataaa gtcggttcca ggtgaagccc
     5521 agaatgcggt cagtcaggcg gatcatctcg taagagaaat tcgccgcaat ctcttccatc
     5581 agcgcaatcg cgttctgctg cgctttttca tgggagattt ttttgctgcg cgcttcatct
     5641 tctaccgctt tggcaatggc gcgggaggcg agcagcttat taaacagatc ctgacgagca
     5701 ggcagacgtg ggcccacagc agccagacgt tgacgggcaa agtgcatacg cgccacgcgc
     5761 gccagtttct gtgcgatagt tttatccgtg ccgtgttcat ccgccatacg gcgcagcgaa
     5821 actgacggcg agaaacgcac aaaactgtcg cgaccgagcc acagtacggc gaaaaatttc
     5881 tgtacgccgt taagcatacg cagcggcggg tttacttcgc ctttttcacg ccccggcgcg
     5941 cgaccaaaca tcaccgatac tggcaccatc tgcacatcca gatttgggtt gctacggtgc
     6001 aaatcgagat agtcgtggaa cagcttaata gactcttctt tcggcgtgta ataggtgaat
     6061 acacgcggcc cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc gatttccagc
     6121 ggctctaacg ggtcaggcaa gtcatgagcc agacactggg cgcgtaacgt cagcaaatct
     6181 gctttcgagt tgtacggtaa aacgtacata attggacgag aagtatccag ccccagttcc
     6241 ggggcaggat ctgccggaat agacttgctt tttaccagga tgcttaatgg taaattcagt
     6301 aatttgtagt aaattcgtgg ccagccggac ataaacgatg taaagcctct ggttaataat
     6361 gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt
     6421 tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg gtacactgat attgacgctc
     6481 ataatgtaaa aaggttcttt caatggccaa taataccact ggattcaccc gaattatcaa
     6541 agctgctggc tattcctgga aaggtttacg cgctgcatgg atcaacgaag cggcattccg
     6601 tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc
     6661 aattacccgc gtgctgctta tcagctccgt gatgctggtg atgattgtgg aaattctcaa
     6721 tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa taccatgagc tttccggacg
     6781 cgctaaagat atgggatccg ccgcggtgct gattgccatt atcgtcgccg tgattacctg
     6841 gtgcattctg ttatggtcgc attttggata acccttccag a
//
LOCUS       DAFATA010000157         6877 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFATA010000157 DAFATA010000000
VERSION     DAFATA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.159"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..843
                     /gene="galM"
                     /locus_tag="LGJ20_004436"
     CDS             <1..843
                     /gene="galM"
                     /locus_tag="LGJ20_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBK2388428.1"
                     /translation="AFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQLHGGPEGF
                     DKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITYRATVDKPC
                     PVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGTSFDFRSAK
                     IIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYTTAPALQFY
                     SGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEEYSSLTEYQ
                     FIAE"
     gene            1048..1800
                     /gene="gpmA"
                     /locus_tag="LGJ20_004437"
     CDS             1048..1800
                     /gene="gpmA"
                     /locus_tag="LGJ20_004437"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK2388429.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(1958..3010)
                     /gene="aroG"
                     /locus_tag="LGJ20_004438"
     CDS             complement(1958..3010)
                     /gene="aroG"
                     /locus_tag="LGJ20_004438"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBK2388430.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            3326..3706
                     /locus_tag="LGJ20_004439"
     CDS             3326..3706
                     /locus_tag="LGJ20_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBK2388431.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3820..4761
                     /gene="zitB"
                     /locus_tag="LGJ20_004440"
     CDS             3820..4761
                     /gene="zitB"
                     /locus_tag="LGJ20_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBK2388432.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(4758..5477)
                     /gene="pnuC"
                     /locus_tag="LGJ20_004441"
     CDS             complement(4758..5477)
                     /gene="pnuC"
                     /locus_tag="LGJ20_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBK2388433.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(5515..6558)
                     /gene="nadA"
                     /locus_tag="LGJ20_004442"
     CDS             complement(5515..6558)
                     /gene="nadA"
                     /locus_tag="LGJ20_004442"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBK2388434.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         1731 a         1752 c         1688 g         1706 t
ORIGIN      
        1 gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag ccgttatacc
       61 tttgacggtg aaaccgtgac gctttcgcca agtcagggcg ttaaccagct gcacggcggc
      121 ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg tcaggtgctg
      181 tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg cgcgacggtg
      241 caatatcgtc tgaccgacga taaccgtatc tctattactt atcgcgccac agttgataaa
      301 ccttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga gcagtctgac
      361 gtgcgcaatc acaagttgca gattctggcg gacgaatatc tgccggttga tgaaggcggc
      421 attccgcacg acggtctgaa atctgtcgcc ggaacgtctt ttgatttccg tagcgccaaa
      481 atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg ttacgatcac
      541 gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt ctggtcagca
      601 gatgaaaaat tgcagctgaa ggtctacacc accgctccgg ctctgcaatt ctactccggc
      661 aacttcctcg gcggcacgcc atcaagagga actgaacctt acgccgactg gcaaggcctg
      721 gcactggaaa gcgagtttct gccggacagc ccaaaccacc ctgaatggcc gcaaccggac
      781 tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt tattgctgag
      841 tagcacctga agccctcgta atgagggctt tatccttata ttgctcaaat caccagcaaa
      901 caccgacata tttgcaactc aatattcaca acaaccttac actgcgacac tattttcgct
      961 atggttatgc gtaagcattg ctgttgcttc gtcacggcaa tataatgaga attattatca
     1021 ttaaaaaatg atttgaggag taagtatatg gctgtaacta agctggttct ggttcgtcat
     1081 ggcgaaagtc agtggaacaa agaaaaccgt ttcaccggtt ggtacgacgt ggatctgtct
     1141 gagaaaggcg taagcgaagc aaaagcagca ggtaagctgc tgaaagagga aggttacagc
     1201 tttgactttg cttacacctc tgtgctgaaa cgcgctatcc ataccctgtg gaatgtgctg
     1261 gacgaactgg atcaggcatg gctgcccgtt gagaaatcct ggaaactgaa cgaacgtcac
     1321 tacggtgcgt tgcagggtct gaacaaagcg gaaactgctg aaaagtatgg cgacgagcag
     1381 gtgaaacagt ggcgtcgtgg ttttgcagtg actccgccgg aactgactaa agatgatgag
     1441 cgttatccgg gtcacgatcc gcgttacgcg aaactgagcg aaaaagaact gccgctgacg
     1501 gaaagcctgg cgctgaccat tgaccgcgtg atcccttact ggaatgaaac cattctgccg
     1561 cgtatgaaga gcggtgagcg cgtgatcatc gctgcacacg gtaactcttt acgtgcgctg
     1621 gtgaaatatc ttgataacat gagcgaagaa gagattcttg agcttaatat cccgactggc
     1681 gtgccgctgg tgtatgagtt cgacgagaat ttcaaaccgc tgaaacgcta ttatctgggt
     1741 aatgctgacg agatcgcagc gaaagcagcg gcggttgcaa accagggtaa agcgaagtaa
     1801 acgtcattcg tttaaaatga gaaagccgac tgcaagtgag tcggcttttt tgttgctaac
     1861 aatggagcac attgcctgat gcgacgctgc gcgacttatc aggcctgtgg tgattcatcg
     1921 gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg
     1981 cattcgccag ttgacgtaac agagcatcgg tatcttccca gccgatgcag gcatcggtga
     2041 tgctcttacc gtaggccagc ggctccccgc tctcgaggct ctgattgcct tccaccagat
     2101 ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt
     2161 cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg ctgaaatcga
     2221 tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca gcaacgtgct
     2281 tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccgc
     2341 tggtattcac aatcgccgaa tgcccccatt tcgttacgga caggaagcag tgcggcgcac
     2401 cggcggcatt aatggcatcg atagccactt taatcgtacc gtcggtgcca tttttgaagc
     2461 cgaccggaca agaaagccct gatgccagtt cgcggtgcac ctgcgattcg gtggtacgtg
     2521 cgccaattgc gccccagctc atcaggtcag cgagatattg tggggtgatc atatcgagaa
     2581 actcacctgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac
     2641 gcagaccgtc gttgatctgg aagctattat ccatatgcgg atcgttaatc agccctttcc
     2701 agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt
     2761 tcagctcttc acgcagcgcc agcaagcgag tggcatactc ttttgccgcg acaggatcat
     2821 gaattgagca tgggccaatc acaaccaaca ggcgatcatc attacctttc aggatcttat
     2881 ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagtagcg gggaattttt
     2941 ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct
     3001 gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat
     3061 ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga
     3121 attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact
     3181 gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata acctggctga
     3241 aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac
     3301 ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac
     3361 tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa
     3421 taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa
     3481 tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggtg gcacaatgct
     3541 gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa
     3601 caccatgtgc aaagatggtc gctgtccgga tatcaataaa aaggtacaaa ccggtgatgg
     3661 catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc
     3721 caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg
     3781 tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac
     3841 acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg
     3901 actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg
     3961 ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc
     4021 gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc
     4081 acgctcgcgg cgtttgtgaa tgccatagcc ctcgtggtaa ttaccatttt gattgtctgg
     4141 gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat gatggcaatt
     4201 gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa
     4261 gagaaaaacc tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg
     4321 gttggggcta ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc
     4381 attctctcga tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat
     4441 agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt
     4501 cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta
     4561 ggcgagaagc cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc
     4621 ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt
     4681 cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc
     4741 cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg
     4801 atccacatcc ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg
     4861 gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac
     4921 agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc
     4981 atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat
     5041 cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata
     5101 aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc
     5161 aatggcaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca
     5221 taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc
     5281 tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa gaagtagttg
     5341 ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct
     5401 tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag gatattctgc
     5461 acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc
     5521 acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag
     5581 cctttcatca acatgaacct cgtgattgct tccttcctgt tctaatgcct ctgcgatgga
     5641 ctgaaggcca ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcaccctc
     5701 acctgcggtt ggtgcttcca gtaactcttt atctggcact gcctgctgca ttttgtagaa
     5761 aatgccccga tcggttgcca cgataagccg ctgatgtggc aatgttttcg cagcagcgat
     5821 cagttgactg gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg
     5881 atgcaccagt atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt
     5941 aaattcatca tgcacaatac aggcaccctg ccagcataga atgtctccac ccgtctgttt
     6001 ttgcacgtaa cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc
     6061 aagatgatca ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt
     6121 taccgcagca gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa
     6181 tgcgttaaat tcttcaacag ggcagccgag atccagtgaa cattcagcct gaagtgtcgg
     6241 catcagaatt gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc
     6301 agcgactaac aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc
     6361 agaaatacag ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg
     6421 ggcaaccatc accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc
     6481 tttttcatca atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg
     6541 atcaaacatt acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt
     6601 agcgtaatat tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat
     6661 ggtggggaaa agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt
     6721 aagctctgac gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa
     6781 agcgccttta gggcgctttt ttacattggt gggtcgtgca ggatgactcg cttcgctcgc
     6841 ccttcgggcc gtcgccgcga gcggctccgt tgtttcg
//
LOCUS       DAFATA010000158         6839 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFATA010000158 DAFATA010000000
VERSION     DAFATA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.160"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..240
                     /locus_tag="LGJ20_004443"
     CDS             <1..240
                     /locus_tag="LGJ20_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="negative regulator of ulaG and ulaABCDEF; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBK2388435.1"
                     /translation="KGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGM
                     LFSRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(237..986)
                     /gene="yjfP"
                     /locus_tag="LGJ20_004444"
     CDS             complement(237..986)
                     /gene="yjfP"
                     /locus_tag="LGJ20_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK2388436.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            1168..1497
                     /gene="bsmA"
                     /locus_tag="LGJ20_004445"
     CDS             1168..1497
                     /gene="bsmA"
                     /locus_tag="LGJ20_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBK2388437.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1646..1921
                     /gene="yjfN"
                     /locus_tag="LGJ20_004446"
     CDS             1646..1921
                     /gene="yjfN"
                     /locus_tag="LGJ20_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBK2388438.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(2038..3663)
                     /locus_tag="LGJ20_004447"
     CDS             complement(2038..3663)
                     /locus_tag="LGJ20_004447"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBK2388439.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3747..4910)
                     /locus_tag="LGJ20_004448"
     CDS             complement(3747..4910)
                     /locus_tag="LGJ20_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBK2388440.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLM
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(4913..5551)
                     /locus_tag="LGJ20_004449"
     CDS             complement(4913..5551)
                     /locus_tag="LGJ20_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBK2388441.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(5561..5959)
                     /locus_tag="LGJ20_004450"
     CDS             complement(5561..5959)
                     /locus_tag="LGJ20_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBK2388442.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(5977..6636)
                     /locus_tag="LGJ20_004451"
     CDS             complement(5977..6636)
                     /locus_tag="LGJ20_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HBK2388443.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(6687..>6839)
                     /locus_tag="LGJ20_004452"
     CDS             complement(6687..>6839)
                     /locus_tag="LGJ20_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBK2388444.1"
                     /translation="RQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSSAQDVLARL
                     QRQQGE"
BASE COUNT         1755 a         1853 c         1624 g         1607 t
ORIGIN      
        1 aaagggctga ccgcagaagg gttgtataaa accgatatgc tgacagcaat ggcagagcag
       61 aagatgctga gcgtggtagg gaaactggtg gtactggttg atagcagtaa gattggcgaa
      121 cgcgcgggaa tgctttttag ccgtgccgat caaatcgata tgctgatcac cggcaaaaat
      181 gctaacccgg aaatcctgca acaactggaa gcgcaagggg tcagcattct gcgtgtttaa
      241 agatgctggc ggaaaaatgt cacggcagca tctaacgcct caggcgtaat gcggtgacgc
      301 acgcctggct gccatgaaca ggttagcagt ttatcccgtc ccgtctcgct taaggcctgc
      361 tgcaaacgta gtgattcgtc ggcaggcaca acgtcatcgt cgaggccatg ccacagcagt
      421 agaggtctgt ccccaagttg ttccaggtgg tttgtcgctt cccactctgc cagcggcgcg
      481 ataatgttat tgaattcatt ctgctgtgct gccgtttcag gtatcagcgg tggaaacagt
      541 gaacgggcga gcgatgtaaa atagcccgat cccatcatgc tggcggtaca tttcaccgtg
      601 gggtggcgag cggtaattcc cagtgccgtc atcgcgccca tcgacgcgcc accgactgcc
      661 agacggtcat caagcagcca gttttcttcg gctattgccg cacgtaaagt agtgaattcc
      721 tgcatacttt gtagcaagat ttgccagaat tgatttaacc gccgcgcttc gtcgctacta
      781 aaacggctac cgtgatcggg cgcatccggc atgatcaccc gcaaaccagc ctgcgccagc
      841 gcaacggcaa aatagctata caccaggctg gatgaagtaa agccgtgata aaaaattacg
      901 cacggtaacg gggtatcttt ttgcccgaca ggataagcat gaagaacggg aatatctgcc
      961 agctcgcgtg attctatttc aatcatctgt ctctcctttt tgtctggtat gacatgaaga
     1021 aatccttagc acaagtttct gctggcggta ttagttcata atgttgagat gtgggttacg
     1081 ctttcgtcac gttttcattc gaaaatcgcg taccaggtaa caattcggga acattccccc
     1141 aaagttaaat taataaggca ctacactatg gttagcagga aacgtaatag cgttatttac
     1201 cggtttgcca gtttattatt ggtgttgatg ttaagtgcct gtagcgcact gcaaggtacg
     1261 ccacagccag caccgccagt aacgaaccat ccgcaagaga ttcgccgcga ccagacgcaa
     1321 gggttacagc gaataggtag cgtaagcacg atggttcggg gttctccgga tgacgcttta
     1381 gcagaaatca gagcgaaagc tgtcgctgca aaagctgatt attacgttgt cgtcatggtg
     1441 gacgaaacca tcgtgacagg acagtggtat tcacaagcca ttttgtaccg taaataaatc
     1501 agacaactta catagtcagc tttacattgc tttgcgtccc tatgcgttta cctgtgcaca
     1561 ataaattacc ggctggaagc ctgggcgttt attaccgcga tggaatgccc tgcaacatgt
     1621 ggggaaacga aaaatggagc tgacgatgaa acaattactt gcctcaccct cgctgcaatt
     1681 agtgacttat cctgcgagcg ccacggcaca gtctgcagaa ttcgccagtg ctgactgcgt
     1741 gacgggcctg aatgaaatag gtcagatctc agttagcaat atttcagggg atccgcagga
     1801 cgtggaacgt atcgtggcgt taaaggccga tgaacaaggt gcgtcatggt accgcattat
     1861 taccatgtac gaagaccagc agcctgataa ctggcgcgta caggcgatcc tgtatgcatg
     1921 agtgtcatct acagcaaatg aacaatgccg gatgcggcgc gagcgccttg tccggcctac
     1981 aattttcaca tatttcattg agttaatcga aaccagcgtc gcatcagtcg tatacgctta
     2041 cacacacact ccccccgtcg cccgcagcaa taaatcattc tggatctgct ctgacaaccg
     2101 tacgccgccg cgcgtatcta acatcacctg acaccacgcc tgcgccattg gcggagaggc
     2161 atatttcaac atttgcgcac cgcagcccag caggaatagc tgatgagtaa tctctcgccc
     2221 cagttcttca gctggcttac gcagctgctg ctgtaaacga cgaaccgcgc gatcaaaata
     2281 gcgatcctgc cctttcactt ccacaaatgc ttccgacaat aagtcgtata cgcccgcttg
     2341 tttattgaga acgcgcagca catccaggca cataatattg ccggaacctt cccaaatact
     2401 gtttaccggc atctcccggt aaagccgcgg cagctcgctc tcctcgcaat aaccaatgcc
     2461 gcccagcacc tccatcgctt cggccacaaa cggcatacct cttttgcaga tcacaaattt
     2521 tgccgcaggt gtaaataaac gcgcccacag agcttctttg gcatcggcac gccggtccca
     2581 cgctcgcgca agacgaaaca gcaacgccgt ttgcccttca agctgaagtg ccatgcgact
     2641 taagacatga cgcataaggg gctgttggat caatggatta ccaaaaacat ggcgttgatg
     2701 tgcatgataa atcgccagcg agaacgcacg gcgcatcatg gcatggctac ccagggcgca
     2761 atcaaaacgc gtcatcccac ccattttcag gatcagacga attccttccc cttcctgccc
     2821 caacaaccag ccaatggcat cctgaaactc cacttcgcag ctggcgttag agcgattacc
     2881 cagcttatct ttcagccgct cgaggcgaat cgcgttgcgt tgcccgtcag gcaaaaagcg
     2941 cggcacaaaa aagcaggaca aaccgcccgt agtctgcgcc agcaccagat gcgcatcgct
     3001 ttgcggcacc gagaaaaacc atttatgccc caccagccga taagagccat cttccaggcg
     3061 ctctgcgcgg gtggtgttgc tcatgacatc ggaaccgccc tgcttttccg tcattcccat
     3121 gccaatcaac aaaccgcgtt tttgcccacc tggcaataag tgagaatcgt agcgatcgct
     3181 cagcagcggc gtggtccagt cctgaaacgg cgtgggtaac atctgtaaca acaatggcgt
     3241 ggcggcaaag gtcatggtta tcggacataa cgaccccgcc tcaacctgcg catgtaacat
     3301 aaaacgcgcc gcgcgcgcca caaatgcgcc ggagcgagcg tcttcttccc aggcaagatt
     3361 gtgcacccga ttggtacata gcgcctgcat cagcaggtgc caggcggggt gaaaacgcac
     3421 atcgtccaga cggcgtcctt gcgcatcgta gcgcaataat tcaggcggat tcacattcgc
     3481 cagccgcccc agttcaaggg attcagccgt tcctaactgc tgaccaatac tggctagaaa
     3541 atcgctatcc cagccagcac cttcacgcgt taccgcttcg cagagcgcgc catcagacag
     3601 gtataagttg ctgttattta atggtatagg ttgattaaaa acggtgtgag tttgccagtg
     3661 cactgtgtct ccctccatca atggcagtca cctttagtat ggtcactgcc atggattcct
     3721 gacagaaaga gattgctaaa acattcttag ccagcaatgt aatgcggaac gaaacgagag
     3781 gtgtctttgg tgatcagcgt gttatcttca cggatcccca ttccgcacgc ttcatcatcg
     3841 acaatccagc taccgatgag tgtgtagcta tcgccaaatc gcggcagagg ttgaaacgcc
     3901 tggtagatca tcggttcatc ggcgtaatca ccatcagcgt ggtcaacgac gttattctga
     3961 ccgtcgaaaa tggtgacgtt gccgccttcg cgcgagtaga tcggtttacg cacatagctt
     4021 tcgccagcgg caatctgcgg tttttcacct tcaaaccagg atgcaagaag gttaggatga
     4081 ccagggaaga agcgccaaag cagcggcatt agccctttat tactcaagat acttttccat
     4141 aacggctcca cccattgctc gcgacgcttg cgcagcagcg gaccgttatc gtcacgcatc
     4201 atccactcca gcggatacag cttaaatgca cgctggatga cattatcatc aagatcggtc
     4261 agtacgccgc cgacgcccaa accgagatct tcaatgtaga taaaccgcga ctcctgccct
     4321 gcctgctggg cgcagtcctg caagtacagc acggtagtcc tgtcttcatc ggtgtcctga
     4381 cagcagcaaa aataaaacgg ttcccgactg taaagctcac taaagcgcga catcaggcgt
     4441 tcctgaatgg cattgtactg atcggcatca cgcggaataa tgccgctgcg ccgggcatcc
     4501 tccagccaca accactggaa ataagccgac tcatacaacg aggtcggcgt atcggcgttg
     4561 tactccagca gcttcaccgg cgcattgcca caccaggcaa aatccatccg gccatacagc
     4621 gaaggatcgc gtgcgcgcca gctttcggcg atcacatccc agtacaacgg cgggatcgcc
     4681 agttgcgtca ggatctcttc gtctttaacc gcgcgatcca ccacctcaag gcacatctga
     4741 tgcagttccg cagtcggttt ttcgatctgc tcttcaatct ggcgcagagt aaaacggtaa
     4801 gcccgactct catcccaata gatttcattg tcgatgatat gaaagtcgaa accgttgtta
     4861 gcggcgatct ggtccagatc ccgtcgcaca ggaacgttgt gtctcagcat gattagcctc
     4921 cccaatgccc acgggcgctg gaagaacgac catagccgcc gcgcgaaacg gttgacgctt
     4981 ttttggtggt aaagccgcct gaagagtaag actctttttt gccagaaccg gagcgccagg
     5041 agtaatcacc ggaagtgttg cgccagacgg ggcgggaagc aaatgaggat ccgccgctgt
     5101 tataaacaaa ctgctcatcg cgatctttac gaataacccg gcttaacaaa aatccagata
     5161 ttaccgggat ccaactctgt tcaacgttgt cgtagtaaca attttcatac ttagactgac
     5221 agttctgctg agtcatattc ttcggaacat cggcataaaa cgccgctttg gcgttattcc
     5281 agccacgcgc gcaaatgtcg gcattattac cgtcatcaat acaatcctgc actgtcgagt
     5341 aaaacgttcc gtcgccgtcg ttatcgacat cgctactttc actccatcct ttgactatta
     5401 aaaaaacggc accgcccatt agcaccaacg ttaagtattt ttgtgcgtag cgattgcggc
     5461 gcggctcaaa aggattatgc ggtcttccaa tgcgactgat cgctccgtga ccaattttac
     5521 tgttatttct tgatttgcgt tttctggcca tgatcgtcca ttaccatgtc atacaagctg
     5581 cgttaaaaat accgccagcc agcgcggcgg ttcccataaa cattcctgct gcggtattgt
     5641 gattaataat tttttcgctt aatgcgggca tataaagtct gacaccagca aaaacgagta
     5701 actgaatcac cagtgcaatc ccgccccagg cgaaatagtc tggaatactc accgcattga
     5761 ttgccgcact ggataatggg ataacgtaac ctaacaatgt accactgaac gccagtgaag
     5821 ccgcggtatt attgtttttg attaactgcc attcgttgtg cggtgtaatt ttagaataga
     5881 taaaaagaaa aataatcacc atagccacgc caataaaaaa ataggcgcta aaggcaagaa
     5941 gtgaatccag tatatgcatt gtaaacgtcc ttgtaattaa ccaataatat gaagtgatgt
     6001 cagcgggata tcgacaccca acgcgcgcga aaataaccac tctggctcgc cgagatcgtt
     6061 aaaagattcc tcaccattta acagcaaata ttcgtaagta tcttcggtta cttggcgttg
     6121 gtagcccatg gtaaaattat ggacctccca tttggcatgg ttttgatttt ctactttttc
     6181 cagcatgtag acgggttcga tatttcccgg ttcttcgctg ttaaaaaatc gctgccagcg
     6241 tttttcctgc cagtttaaga tcattgcccc catcactttg gcgttgatcg cctcgcgcca
     6301 gtgactctct ttactgatac cgtagctctc ttcatagaca aagagcttga tatcatcaat
     6361 gtcatcaata tcttcgccgc cggtggtatt gatttgcaga aattcatcgc ctgaagtgta
     6421 gtagcggaaa atctgactac cgccgcccag atcgatgtgg ctgacagcgg ctaccgtaaa
     6481 ttcctcaccc ggcagcgcaa tcagcaattc atcttccagc aaacgaaacg ctaacgtgtc
     6541 gagcgtaaag ccactattga gatgaagtcc cagcggaccg cgagcgatag caggcttatt
     6601 atccttgcca aacagacgct ggaaaaaacc agacatattt tatccttaaa atagttgcca
     6661 gccttttagc gaggctggca aaaaaattac tcgccctgtt ggcgttgcag tcttgctaat
     6721 acatcctggg cgctactttt attgctaccg ccaattccgg cttccgccag cttttcatca
     6781 agatcgcgac cgtctgcgac tttttccaac tgtgcagcag catccaggcg agcctgacg
//
LOCUS       DAFATA010000159         6834 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFATA010000159 DAFATA010000000
VERSION     DAFATA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.161"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(17..103)
                     /locus_tag="LGJ20_004453"
     tRNA            complement(17..103)
                     /locus_tag="LGJ20_004453"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Leu,seq:cag)
     gene            complement(132..218)
                     /locus_tag="LGJ20_004454"
     tRNA            complement(132..218)
                     /locus_tag="LGJ20_004454"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Leu,seq:cag)
     gene            complement(486..1517)
                     /gene="rsmC"
                     /locus_tag="LGJ20_004455"
     CDS             complement(486..1517)
                     /gene="rsmC"
                     /locus_tag="LGJ20_004455"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBK2388445.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1620..2033
                     /gene="holD"
                     /locus_tag="LGJ20_004456"
     CDS             1620..2033
                     /gene="holD"
                     /locus_tag="LGJ20_004456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBK2388446.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            2002..2448
                     /gene="rimI"
                     /locus_tag="LGJ20_004457"
     CDS             2002..2448
                     /gene="rimI"
                     /locus_tag="LGJ20_004457"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK2388447.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2463..3140
                     /gene="yjjG"
                     /locus_tag="LGJ20_004458"
     CDS             2463..3140
                     /gene="yjjG"
                     /locus_tag="LGJ20_004458"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBK2388448.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3231..4820
                     /gene="prfC"
                     /locus_tag="LGJ20_004459"
     CDS             3231..4820
                     /gene="prfC"
                     /locus_tag="LGJ20_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK2388449.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5213..5818
                     /gene="osmY"
                     /locus_tag="LGJ20_004460"
     CDS             5213..5818
                     /gene="osmY"
                     /locus_tag="LGJ20_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBK2388450.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5945..6106
                     /locus_tag="LGJ20_004461"
     CDS             5945..6106
                     /locus_tag="LGJ20_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBK2388451.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6228..>6834
                     /locus_tag="LGJ20_004462"
     CDS             6228..>6834
                     /locus_tag="LGJ20_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK2388452.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKT"
BASE COUNT         1651 a         1773 c         1830 g         1580 t
ORIGIN      
        1 ttgatatcgc ctcgtttggt gcgagggggg ggacttgaac ccccacgtcc gtaagaacac
       61 taacacctga agctagcgcg tctaccaatt ccgccacctt cgcccagtgc gagcaatatc
      121 aacgtggttt ttggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca
      181 cctgaagcta gcgcgtctac caattccgcc accttcgcat accatcaatt cttaaaaaga
      241 attgctacca cggaggcgca ttctagtggt tttcagcttt tcgtcaatag ttaattatcg
      301 ccggaggtgt aattgctgga aaaatgtcca tcaggaaact agcgtgcagg tttggtaggc
      361 atgcgggggc agatgccaga tgcgacgctg gcgcgtctta tctggcctac gaagggctaa
      421 cgtgcaggtt ttgtaggtcg gataaggcgt tcacgccgca tccgacacgg tattcggcga
      481 gataattaac ctttcttcgc ctggcgggtc ataatggcgc gatacacctt gaaacgcccg
      541 gtttgcgcaa tcacttcatg gaagccaaat gtctcatcca gcacgtccgg gtaaggcagg
      601 aaggcgttcg ctacaattcg cagctcgccg ccgctattaa gatgacgcac cgcgccgcga
      661 atcagcgttt gcgccgcatc caggctggtt tgcatcccat cgtggaacgg cgggttggag
      721 atgatcatat caaaacgacc tttcacctcg gaaaagacgt tgctggcaaa gacttcacct
      781 tcaacacagt tggccgcaag tgttgcgcgg ctggcttcta ccgctggcgc agagacatcg
      841 cacaaggtga gacgaatttt cggcgaatgg cgcgcaaagg caactgaaag cacccccgcg
      901 ccacagccga catccagcac tttacctttc gtgtgcggag ttaacgtcga gagcagcaac
      961 tggctaccga catccagacc gtcgcggcta aacacgccag gcagcgtttt gaccgtcagg
     1021 ccatcgacgc tgtattcgcc ccagaattta tcggcatcaa ataccggctg tttttccaga
     1081 cgaccaaaat agaggccaca gcgacgagcg ctgtcgactt tattcaacgg cgcataatct
     1141 gccagcatct gctcggcgct gcgcacgccg ctgcggttct cgccaacgac aaaaatatcc
     1201 gtccccaccg gcagcagaga aagtaaattc atcaactgga actgggcttc cggtttgttc
     1261 ttcggccagt agtaaatcag cgtatcgcaa tctgcgacgt catccgccgt ggcgaccaga
     1321 ctgaaacggg cgttatcccc catctggcgg cttaatacct gccagtggtg gaattgctgg
     1381 gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca ggtcatcctg taagtctccg
     1441 gcaaacagaa tacggctttg ttcgaaatca tcactgtgac gcagcaagac ttcacttgcc
     1501 ggggtaaatg cagacatgga atgctcctca attgatactg gcggcgatta tagccatatg
     1561 ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg acaacaggag cgattcgcta
     1621 tgacatcccg acgagactgg cagttacagc aactgggcat tacccagtgg tcgctgcgtc
     1681 gccctggcgc gttgcagggg gagattgcca ttgcgatccc ggcacacgtc cgtctggtga
     1741 tggtggcaaa cgatcttccc gccctgaccg atcctttagt gagcgatgtt ctgcgcgcat
     1801 taaccgtcag ccccgatcaa gtgctgcaac tgacgccaga aaaaatcgcg atgctgccgc
     1861 aaggcagtcg ctgcaacagc tggcggttgg gtactgacga accgctatca ctggaaggcg
     1921 ctcaggtggc atcaccggcg ctcaccgaat tacgggcaaa cccaacggca cgcgccgcgt
     1981 tatggcaaca aatttgcaca tatgaacacg atttcttccc tcgaaacgac tgatttacca
     2041 gcggcttacc acattgaaca acgcgcccac gcctttccgt ggagtgaaaa aacctttgcc
     2101 agcaaccagg gcgagcgtta tctcaacttt cagttaacgc aaaacggcaa aatggcggcg
     2161 tttgcgatta cgcaagtggt gctggatgaa gctacattgt tcaatattgc ggtcgatcct
     2221 gactatcagc gtcagggatt gggaagggtg ctgctggaac atctgatcga cgaactggaa
     2281 aaacgcggcg tggcgacact atggctggaa gtccgtgcct caaatgctgc cgccattgcc
     2341 ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc gcaattacta ccccaccacg
     2401 gacggtcgcg aagacgccat catcatggcg ttgccaatca gtatgtaaga caaggtggaa
     2461 taatgaagtg ggactggatt ttctttgatg ccgatgaaac gctgtttacc tttgactcat
     2521 tcaccggcct gcagcggatg tttcttgatt acagcgtcac ctttaccgct gaagattttc
     2581 aggactatca ggccgttaac aagccactgt gggtggatta tcaaaacggc gcgatcactt
     2641 cattacagct tcagcacggg cgttttgaga gctgggccga acggctgaaa gttgaagcag
     2701 gcttgcttaa cgatgccttt attaatgcga tggcggaaat ctgcacgccg ctgccgggcg
     2761 cggtttctct gcttaacgcc attcgtggca acgccaaaat cggcatcatc accaacggct
     2821 ttagcgcctt gcagcaagtg cgtctggaac gcacgggcct gcgtgattac ttcgatttgc
     2881 tggtgatttc cgaagaagtt ggcgttgcca aaccgaataa gaaaattttc gattatgcgc
     2941 tggaacaggc gggcaatcct gaccgttcac gcgtgctgat ggttggcgac actgccgagt
     3001 ccgatattct cggtggcatc aacgccgggc ttgcgacctg ctggctgaat gcgcaccatc
     3061 gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc ttcgttgcac gaactggagc
     3121 agctcctgtg taaacactga ttgcctcccc cccgttgatg ggtaaaatag ccgcaatttt
     3181 tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga agaaagaatt atgacgttgt
     3241 ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc cattatttct cacccggatg
     3301 ccggtaagac taccatcacc gagaaggtgc tgctgttcgg acaggccatt cagaccgccg
     3361 gtacagtaaa aggccgtggt tccaaccagc acgctaagtc ggactggatg gagatggaaa
     3421 agcagcgtgg gatctccatt actacgtctg tgatgcagtt tccgtatcac gattgcctgg
     3481 ttaacctgct cgacaccccg gggcacgaag acttctcgga agatacctat cgtaccctga
     3541 cggcggtgga ctgctgcctg atggttatcg acgctgcaaa aggtgttgaa gatcgtaccc
     3601 gtaagctgat ggaagttacc cgtctgcgcg acacgccgat cctcaccttt atgaacaaac
     3661 ttgaccgtga tatccgcgac ccgatggaac tgctcgatga agttgagaac gagctgaaaa
     3721 tcggctgcgc accgatcacc tggccgattg gctgcggcaa gctgtttaaa ggcgtttacc
     3781 acctttataa agacgaaacc tatctctatc agagcggtaa aggccacacg attcaggaag
     3841 tccgtattgt taaagggctg aataacccgg atctcgacgc tgcggttggt gaagatctgg
     3901 cacagcagct acgtgacgaa ctggaactgg tgaaaggcgc gtctaacgag ttcgacaaag
     3961 aactgttcct tgcgggcgaa atcactccgg tattcttcgg tactgcgctg ggtaacttcg
     4021 gtgttgatca tatgctggat ggcctggtgg agtgggctcc tgcgccgatg ccgcgtcaga
     4081 ctgatacccg taccgtagag gcgagcgaag acaaatttac cggcttcgta tttaaaattc
     4141 aggccaacat ggacccgaaa caccgcgacc gcgtggcgtt tatgcgcgta gtatccggta
     4201 aatatgaaaa aggcatgaag ctgcgccagg tgcgtactgc gaaagatgtg gttatctccg
     4261 acgcgctgac ctttatggcg ggcgaccgtt cgcacgttga agaagcgtat ccgggcgata
     4321 tcctcggcct gcacaaccac ggcactattc agatcggcga cacctttacc cagggtgaga
     4381 tgatgaagtt caccggtatt ccgaacttcg caccagaact gttccgtcgt atccgcctga
     4441 aagatccgct gaagcaaaaa cagctgctca aagggctggt acagctttcc gaagagggcg
     4501 cggtgcaggt gttccgtccg atctccaaca acgatttgat cgttggtgct gttggtgtgc
     4561 tgcagtttga tgtggtggta tcgcgcctga agagcgaata caacgttgaa gcagtatatg
     4621 aatcagtcaa cgttgccact gcccgctggg tagaatgtgc ggacgcgaag aaattcgaag
     4681 agttcaagcg taagaacgaa agccaactgg cgcttgatgg cggcgataac ctcgcttaca
     4741 tcgctaccag catggtcaac ctgcgcctgg cgcaggaacg ttatcccgac gttcagttcc
     4801 accagacccg cgagcattaa ttcttgcctc cagggcgcgg tagccgctgc gccctgtcaa
     4861 tttcccttcc ttattagccg cttacggaat gttcttaaaa cattcacttt tgcttatgtt
     4921 ttcgctgata tcccgagcgg tttcaaaatt gtgaactata tttaacaaag taatgacatt
     4981 tctgacggcg ttaaataccg ttcaatgcgt agatatcagt atctaaagcc gtcgattgtc
     5041 attctaccga tattaataac tgattcagag gctgtaatgg tcgttattca tcacccatcg
     5101 cttttgtgat ggcgaccatt gacttctgta gagggtgaag tctctcccta ttcagcaatg
     5161 caacctcgtg ttgccaggct caaattacga gcaaacatac aggaataaat cgatgactat
     5221 gacaagactg aagatttcga aaactctgct ggctgtaatg ttgacctctg ccgtcgcgac
     5281 cggctctgcc tacgcggaaa acaacgcaca gactaccaat gaaagcgcag ggcaaaaagt
     5341 cgatagctct atgaataaag tcggtaattt catggatgac agcgccatca ccgcgaaagt
     5401 gaaggcggcg ctggtggatc atgacaacat caagagcacc gatatctctg tcaaaaccga
     5461 tcaaaaagtc gtgaccctga gcggtttcgt tgaaagccag gctcaggccg aagaggcagt
     5521 gaaagtggcg aaaggcgttg aaggggtgac ctctgtcagc gacaaactgc acgttcgcga
     5581 cgctaaagaa ggctcggtga agggctacgc gggtgacacc gccaccacca gtgaaatcaa
     5641 agccaaactg ctggcggacg atatcgtccc ttcccgtcat gtgaaagttg aaaccaccga
     5701 cggcgtggtt cagctctccg gtaccgtcga ttcccaggca cagagcgacc gtgctgaaag
     5761 tatcgccaaa gcggtagatg gtgtgaaaag cgttaaaaat gatctgaaaa ctaagtaatt
     5821 cgtcgtaatt cgtcctcccg aactttgtcg ggaggcgcga tgtgcaccac actaaaaata
     5881 tcgcattgag tgattttcac gctcatatca agcggtgaca ttaactatgg taaaggagac
     5941 gcttatgttt cgttggggca tcatatttct ggttatcgcg ttaatcgccg ccgcacttgg
     6001 gtttggtggt ctggccggta ccgctgcagg cgcagctaaa attgtctttg tcgtcgggat
     6061 tattctgttc ctggtgagtt tgttcatggg ccgaaaacga ccctagattt cagaccatta
     6121 agaaaatttt atccaaagcc agtccagcgg actggctttt gcggttttag cgaataataa
     6181 attgcgttac tttgtcattc tttcacaaca aaaacaggaa ggcagaggtg gggcagcgaa
     6241 tacctgtaac gcttggtaat attgcgccgt tgtcgctaag gccgttccag cctggacgaa
     6301 tagctctggt gtgcgaaggc ggcggacagc gtggaatttt cacggctggc gtgctggatg
     6361 agtttatgcg cgcgcagttt aatcctttcg atctttatct cggcacatct gccggggcgc
     6421 agaacctctc ggcgtatatc tgcaatcagc ccggttacgc gcgcaaagtc atcatgcgct
     6481 ataccacaaa acgcgaattt ttcgatccat tgcgctttgt ccgtggagga aatcttatcg
     6541 atctcgactg gctggtggag gccaccgcaa gtcagatgcc gttgcaaatg gataccgccg
     6601 cgcggttgtt tgacagcggc aaatcgtttt atatgtgcgc ctgtcgtcag gatgactacg
     6661 cgccgaatta ctttttacca accaaacaaa actggctgga tgtgattcgc gcctccagtg
     6721 cgatacctgg cttttatcgt agcggagtgt cgctggaagg cattaactac ctggatggcg
     6781 ggatcagtga tgcgattccg gttaaagagg cggcaaggca gggcgctaaa acgt
//
LOCUS       DAFATA010000160         6808 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFATA010000160 DAFATA010000000
VERSION     DAFATA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.162"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(161..472)
                     /locus_tag="LGJ20_004463"
     CDS             complement(161..472)
                     /locus_tag="LGJ20_004463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388453.1"
                     /translation="MEIVIYITISILILDIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     K"
     gene            complement(587..796)
                     /locus_tag="LGJ20_004464"
     CDS             complement(587..796)
                     /locus_tag="LGJ20_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388454.1"
                     /translation="MARRELELREIPYIKNSLHANYSYKSISIGSKQGWLISAKLKVP
                     ETFEPDMIFIEISDPEGFINIPDVL"
     gene            complement(835..5697)
                     /locus_tag="LGJ20_004465"
     CDS             complement(835..5697)
                     /locus_tag="LGJ20_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
                     /protein_id="HBK2388455.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARQGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWL
                     YRYDERGLFSNITDPAGQTWTQLCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYVLDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVISPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQLTSQRAGLFPEEATWGGLLPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETERYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDPTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLY
                     EVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGL
                     REKFMMGLTN"
     gene            complement(5717..6178)
                     /locus_tag="LGJ20_004466"
     CDS             complement(5717..6178)
                     /locus_tag="LGJ20_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HBK2388456.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(6206..>6808)
                     /locus_tag="LGJ20_004467"
     CDS             complement(6206..>6808)
                     /locus_tag="LGJ20_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388457.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTTIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT         1333 a         1989 c         1766 g         1720 t
ORIGIN      
        1 ggcttgtctg taatcgggaa taatagaaat atgttccatc aattttttaa gttccatttt
       61 gttctcctta attatgtaag gagtatttga tcatgtataa gcaataaaaa acagcttcag
      121 gtaatgagga atatctcaat ttttaaacat aaaatgtgaa ttacttagta caaaaagtgt
      181 acttctgatg atctttccct gtatacatcc ggtctgcggg gctgtgtgcc aggcggtcat
      241 cgtcggtatc gccggtcacc tggtggcgca gacgcattcc cggaaaggcg tcataaaaga
      301 agcggcagct cagggcatca cggtcgaaca gcaccaggta atcatcgcgg cggcgcagga
      361 tgtactgggc gtgatacggg ttactggttt cgatattggc taacatccgc tgttttatta
      421 atgcgatatc taaaatcagg atggaaatag tgatgtatat cactatttcc atgatcttgc
      481 cttgcgttca tccaggcagg aatatccctg ttagcagcct ctgtgcgatg tacgccagcg
      541 gtctgtaaag aaaccgatta ccttataaca aaacctctaa cgtagtttat aatacatcag
      601 gaatattaat aaaaccttct gggtcagata tctcaataaa tatcatatca ggctcaaaag
      661 tttcagggac ttttaatttc gctgaaatta accaaccttg cttagaacca atactaatag
      721 atttatagct gtaattggca tgtagactat ttttaatata aggaatttcc cttaactcta
      781 actcacgccg agccaagtca agagccattt cgatagttat tttgttatgt tttatcaatt
      841 tgttagtccc atcatgaatt tttctctcaa tccctcaaca tattttatgg catgagctat
      901 ttccgcttca ttgaataaat ttttactttt taatatttca tttagaacgt gttcctccct
      961 agataaacgt cctagtgctt cgtatgcttc tcgtccaatt ttcgcaaatt gcttagcatg
     1021 aatcacttca tgatacacct catataaggt agcatttttc ctaagataaa tattgcgaag
     1081 gccataatca aaagctgccc tagcatttgg tggcaaatat ttatctgctt tccttattac
     1141 tttaattcct agcttctcca tttctttagc aatattgttt atttgactac gtgataaacg
     1201 aaccccatcc aggaaacccg tatgcccact aacacgacga ccatcgccaa tatagtccat
     1261 caaactgctg attttagaac ttgcagccaa tcccagcgga tcaacccacc cacaaggatt
     1321 cggcacatac cggtaagtct gctccccgcc cagcaacccc agcggatcac tcaccaggta
     1381 cattccggtt tccggctcgt aataccggaa ccggttatag cacagccctg attccgcatc
     1441 ctgccactgt cctgcataca gcaggccggg gtcggcttcc gggtccagtt ccccgcgcgg
     1501 gtccgggtag ccggtgtctg cgggcagact gagtgccagc ccccacaggc tggtctgccc
     1561 cggtcgccag acggttttac cttcactgtt aaagagcatc agcgggcggc ccgtcgggtc
     1621 gctcaccgcg tgattcgtcc gcgtcaccca ctgcggggcc acggacgggt gcggctgccg
     1681 tacccggctg aactgctggc ttatcagctc aaagccgtta aacaccaggt gccttacgct
     1741 gtacagttta tcatcgcggt attcccggat ttccgcaata ctgtcgccgt cccacagata
     1801 cgtgaagcgg attttcttcc ggtcgcagcg tttctctgtt cgtctgccgc tggcgtcgtg
     1861 gcggtattcc cactgctcac cctgtgcgct gcaatacccg gtcagctggt cacggctgtc
     1921 ccaccggaac cgctctgttt ccgggcgata gccgtcacgg tgttttgtcc ggctgaccat
     1981 ccggcctgcg gcgtcatagt catactgcgt gtttccggcc tgtttcagcc ggtggcctcc
     2041 ggcataccgc tcagtctctt cacttatccg gtgcctgccc gcagactgcg ccttcaggta
     2101 gccacaactg tcatagccat aggactcatc gccttcgcca tagcccagcc cggtgcctga
     2161 ggctgtcacc gccgtgacct ggccgttccg gtccagccgg tattcccgcc gtgtttcccg
     2221 tccgtaatct tcccggctgg tgacgcctga cacattaccc gcgctgtcgt aacggtactc
     2281 ccgtaccagt gaggggagca gcccgcccca tgtggcttct tccgggaaaa gtccggcgcg
     2341 ctggctggta agctgaccgg ctgcattcca gccctgttca caggcaaaac cccggttact
     2401 gtgccggagc acttcacgtc ccgcctcatc acggctgaac gtcagcgtct catcccccgg
     2461 caggataacc tccgtcagtt ctccggcggc gttgtaacgg taggtggttt tcagcaggga
     2521 ttcatcggga tgtctgtcgc gttcatcttc cggtggcagg atacagcgga tgacggtgtg
     2581 ttcgtcgggg tagtggtatt caatggcccg tcggccctga gtggtgacat tcacccgccc
     2641 gcgttcgtcg tagccggtgc gctgtgatgt gccgtccggg ctgaagatac ccgtcagcct
     2701 gccggatttg tcataggcat actgcgtttt cccttccggc gagatgactt ctgccaggcg
     2761 gcctgcggca tcccggctgt agaggatggt ttcctccagg ccgttaaccc tggcggtaca
     2821 gaggccatcg gcgtcatagc ggtaacgcca gaccacgccg ccccagtcac gctcttccgt
     2881 cacccggccg cagttatcca gaacataaag gtatgattcg ccctgcgggt taatgacttc
     2941 tgtcagttgc agcgtgtctt tatcgtagcg gtactgccag cggtggcctt caccgtccgt
     3001 tcgcgccacc ggcaaatcaa acgggccgta ctccattgtc cagacaacac cgttaccgtc
     3061 cgtgtacgcc agcaggttgc cgtggcggtc atagcggaat ccttccgcgc cggttccagc
     3121 acgttcaatg ccgctcagtt tatggtgctg ctgccactgt aaccgcactg actgaccgcc
     3181 ggggccgcgc atcttcaccg gcaggtcgtg accgctgtat tcggtgtggt gttcacggcc
     3241 catcacgtcg gtgagtgttt ccggccggtt gtggtggtcg tactctatgc ccagacgccg
     3301 ttcatcctgg cggaatattc ccgacaacag cccctgagcg gtgtacgcca gccgggtctc
     3361 ttcgccctgt ggcgacacca ggcttaccgg caggccgcgt tcatcgcaca gctgtgtcca
     3421 cgtctgcccg gcagggtcgg tgatgttgct gaacaggccg cgctcatcat agcggtacaa
     3481 ccactccgcg ccgtctgccg ccgtgtaacg gcttaactga ccgtgtgcgg cgttgtattc
     3541 aaacttctcc acccgcccgg caggggagtg gcgggccagc aggtgatgga attcatccca
     3601 ttcatacgtg gtggtgctgc cgtccggcgc ttcttcccgc agaatattgc cgtcaggatc
     3661 atagtaataa cggtgaatgc ccttgccgtc cgtcacggtg gtgcagccgt caccatagtc
     3721 cagcgtggcg ttgtaatagc cctccgcccc ggtgacattc acgcaccggc cctgtgcatc
     3781 gtaggtgaaa cggctccacg tctggtcgtt atcggaccag cggatgatcc ggtccgcagc
     3841 gtcgtactca taaaacaggt ggtagtccag ccgcgcatcc gcttctgtca gccgcccccg
     3901 cgcatcctgt tcgtaggtcg ccagcgtctg tatgccgtta tccgtgcggt gaatggcttt
     3961 caggtagccg ctggcatggt acgtcagttt aagccggatc ccgtcggtgt gactgacacc
     4021 cgtgatccgg tactggctgt cgcgctcaaa cgtgatgcgg ttgctggcgg tgtcactcat
     4081 cccgcccagc atatacatcc ggtcaccggg gctgtgtgcc aggcggtcat cgctggtatc
     4141 gtcagtcacc gggtggcgca gacgcattcc cggaaaggcg tcataaaaga agcggctgct
     4201 cagggcatca cggtcgaaca gctccaggta atcatcacgg cggcgcagga tgtactgggc
     4261 gtgatacggg ttaaccgccg tatcactttc accgtcgaag gcgaagttca gcgtggctcc
     4321 caggctgatg atatccaccc ggtttccctg ttcacgcacc caggcgtatt cgctccagga
     4381 gtcgctccag cccacgccgg tcaggccatg cagcggcagt acagaactgt ggaaacgggt
     4441 gaaggagagg gggagtgtct ggcccagggt gaagtcggta cgttcgtcgc agtacgcgcc
     4501 ggttaccgga tcgacaggat ctgttaccca tctgatgact ttattggcaa tactttttaa
     4561 cgacttacct gttttcccca gcaacgcgcc gagctttgcc cccagcagct tcccgctttt
     4621 ccccagcagg ttccccagtt tgccgagtct cgccagattc ttcagttttc ccagcagtcc
     4681 cgctggcggc agaagatagc tggctatcgt tattcccccc aggatcattc tctgccacgg
     4741 cgggaactcc ggctggatat ccagataggt cacctgctca ccgccaataa agacgtccgg
     4801 cgaacctccc acgattgctg cgctgcactc cagtttgtcg ccctggcgtg cggcaggctg
     4861 attgttgata aagacgttgc tgctgccctg ggctatcctc ggtggtttgt tctccttgtc
     4921 gcagagcgcc gtgtcctcac cggcttccgg gaaatccaca tcttttttga tttcgccacg
     4981 cccgcccacc atggtggcaa actccattaa taccggaccg gcgggaaaca gaagcgatgc
     5041 gccggcgctg acgcgatcca taaaaccggc attgctgtcc gtcagggtga taacagcgtt
     5101 cccccaggcc ttcgccagtg gtactgcttt ttccgctgcc gattcaagca ggtcagtggc
     5161 aatatcaccc catgagggtt ccccctgtgg gacttcaggt attgctccag gtgggggagg
     5221 agggggaagg gtgacggcgg ccctggcagc gggctttccg ttaattttta cgttttcaga
     5281 accggtattg atggcgccgt ccgtgtcaca aaaactgttt atccatgact caagctggtt
     5341 ctgtttctcc tccacataat cactcgcctt atagctggca tacatcagcg caggcaccag
     5401 aaccagcgca taagggccgg tggcacaggc ggccataatc aggcaacctt ctgcgatggt
     5461 cactatcgcc cccagcacgg cccctgccag cgcccccaaa aaactcttgt gctgaatttc
     5521 atcgcccagc ctggcggcgg gggggccaga cttttttgat gccagcatct gtgcaatggc
     5581 tttgtcgttg gctgcccgtt tcatctggct ggcgaccatc gcctgctgtg acagcattcg
     5641 cattgccagc gtaccgcctg cggtcgctcc ctgtggcccg cctggtcctt cactcatgtg
     5701 ttatccctcg tttatgtcag cgcggctggt ccttttggat gtcattctcc ggtggcaacg
     5761 gggtgaagct ctttatcacc tccagcagca tcgtcttttg actgttcttc agggataagg
     5821 aggtgctgaa agtgaaggtc agcagcgtat ctccacgccg ctggaatacg gctatctggc
     5881 taatccgccc ctgattcccc tcatcttccg gaccgttcca ggaataatcc agcagccagg
     5941 ccggggcctc tgccagagtg atttcccggc aggcatgttc ggtgtaccca tcaagtttgc
     6001 cctggtacag cgccagcgtt tcctggtaca gctctggtgt cagggttccc tgtggaacgg
     6061 cttcgcgggt aatgaccagg ttgatgccac tgttatctgt ggacaccagg acctgcattg
     6121 actgatcctg ccattgagac ggcagttgaa tgtggccttc attaaaagtg aactgcataa
     6181 aatactccct gataatctga gtgaatcagt tgatgaaaac agaaccgccc acgatggtga
     6241 tgccatcctt cgacagcctg attaccgtcg attccccgac ctgtagctga atgccatccg
     6301 gcgtcagcgc aattttgctt tcctccacgg ccagcaaaat ttccttaatc gtcgtataag
     6361 cctgtatacc gtcgccttcg ccaccattgt ttacagcgtc aacatgcacc gaaccggcaa
     6421 tcgtgcctct gtcctgaaag acatcctcag ataaagaccc tttcagtact ccggtgatct
     6481 tgtggccttt ttccaccgta agaaggcggt taccttcctg gatcgtgacc gtctggtcat
     6541 tttttacggt ttcgcggtga tcgtgtttta cggtggtggt acggtcgttg agcacttcgg
     6601 tatccatgtt cttctgcgcg tggatgtaga cctgttctct ccctgtcgca tcatcaaact
     6661 tcagctcatt aaatccgctg cccatgtacg ttttggagcg gatggtcatc tgcgtctttg
     6721 ttcccggcag gctgccgggg gtgcggtttt cctggtggta ggtgcgcccc ataatgatcg
     6781 gctggtccgg atcgccgttg aggaagtc
//
LOCUS       DAFATA010000161         6769 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFATA010000161 DAFATA010000000
VERSION     DAFATA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.163"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(43..981)
                     /locus_tag="LGJ20_004468"
     CDS             complement(43..981)
                     /locus_tag="LGJ20_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBK2388458.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(1001..1765)
                     /locus_tag="LGJ20_004469"
     CDS             complement(1001..1765)
                     /locus_tag="LGJ20_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBK2388459.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            2081..2992
                     /locus_tag="LGJ20_004470"
     CDS             2081..2992
                     /locus_tag="LGJ20_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK2388460.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(3035..4186)
                     /locus_tag="LGJ20_004471"
     CDS             complement(3035..4186)
                     /locus_tag="LGJ20_004471"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBK2388461.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(4200..5795)
                     /locus_tag="LGJ20_004472"
     CDS             complement(4200..5795)
                     /locus_tag="LGJ20_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBK2388462.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(5939..6697)
                     /gene="aroD"
                     /locus_tag="LGJ20_004473"
     CDS             complement(5939..6697)
                     /gene="aroD"
                     /locus_tag="LGJ20_004473"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK2388463.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT         1658 a         1693 c         1614 g         1804 t
ORIGIN      
        1 ttcccgctgc gtttacagcg ggagatgtgg ttgaaagggg aattagcggc tcaactggct
       61 aatcagggca gggacgactt tgtagatatc gcccaccaga ccgtagtcgg cataattgaa
      121 gattggcgca tttttatctt tattgatggc gacaatcact tttgcgccgt tgccgccaac
      181 catatgctgg atctgcccgg agatccccag cgtcaggtag agatcggatt tcagcaacac
      241 gccggagaca ccgatataac gttcacgctc catccagttc tcgccttcgg caattggacg
      301 tgaacagccg acttcagcat tcagcaccgc cgccagttcg tggaccattt tcaggtcatc
      361 ctgcgccgcc agaccacgac cgacgccaac cacacgtttt gctttgctca ggtccacgct
      421 gcttgcggct ttcgcacggc gttcgcgaca gagaatttca tgacgcggag caacataagg
      481 taccgtttct gtcgggcact gatgagaggt atcactggtg cacggttcct gaacaccggg
      541 tgcaagggta atgatcgcca gcgggctgtt gatcttttcc tgagcgaacg ctaacccgcc
      601 atacatccgg tgttcggcgc aaatgtgacc atcgataata tccaccgccg tggcatcgtt
      661 caccagcgcc gcattcagtt gcacacttaa ccgtgctgcc agagctttac cacgtttcgt
      721 cgcggccaac agcaacatag cggggtgttt atctttcagc agggcagcaa tgctttcggc
      781 gtaattttca gtgcgttgca gcgcgtcgtt ttgctcaaga acataaatac attttggacc
      841 ataaggcata actgcctgcg cctggtcggt attttgtaca atggcataca cctgttggcc
      901 ccattgctga gcgccgccaa acagttcagc ataacgttca ggattatcgc taaagaccca
      961 gacgctgttt aattgactca tcgtttcttc tccgtaggct tcagttcagg gctttcttca
     1021 gatgttccgc cagctcggca atggcctccg gcgaatcgtt atcgataatg atgtgcttac
     1081 gttctgtttg cggcggtacg cgaatgccga ccagttcagc aagtggcgcg ctctggctcc
     1141 agtcaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg agaatggctt
     1201 tcatcgaagg aatgcgtggc acgttaatat cggaggtgac gcagagcacg gctggaacag
     1261 agagttcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt gtattgccct
     1321 gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct ccgaccagca
     1381 agccagcctg ctgggcataa aggtcgccgg aaccttcacc aaagatcagt aaatcgaagc
     1441 cgatcttttc aactgctgcc gccagcgctt ttgcggtatc gagaggcagt gcatgttcaa
     1501 gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg tctttgcgca
     1561 ctttcgagtt ctgcaacaat gagccgccaa cggtcagcgc ggctatctca tcgtcatctg
     1621 ttgcgagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt ttggcgtcgg
     1681 cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct tcaggcacca
     1741 gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg aaccatctcc
     1801 gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt tttcgacgga
     1861 gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa aacaaaagtg
     1921 caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt gtaaataatc
     1981 ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat tttatccggc
     2041 atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac gctgttttga
     2101 taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca cggctttcac gttttgcaat
     2161 tagcgatgat ccaaaatggg agtctggaca tcacgttcat gacaatgaaa ccgagctgat
     2221 ttacgtcaag aaaggggtag caaggtttac catcgattca tcgttatatg tcgcacatgc
     2281 cgatgatata gtggtgatag aacgcggcag gctgcatgcg gtggcctctg acgttaacga
     2341 tccggcaacg acgtgtacct gtgcgctgta cggctttcag tttcaggggg ctgaggaaaa
     2401 tcagctactg caaccgcatt cttgtccggt aattgccgcg gggcagggaa aagaagtcat
     2461 aaaaacctta tttaatgagc taagtgtgat tttgccgcaa agtaaaaata gccaaacatc
     2521 ttcgttatgg gatgcatttg cctatacgtt agccattctt tactacgaaa attttaaaaa
     2581 tgcttatcgt tcggagcagg ggtatattaa aaaagatgtt ctgataaaag atattctttt
     2641 ctatctgaat aataattatc gcgaaaaaat cactttagaa cagttatcga aaaaatttcg
     2701 tgccagcgtc agttatattt gccatgaatt taccaaagag tatcgtattt cccctattaa
     2761 ctatgttatt caacggcgta tgacggaagc aaaatggtca ctcactaata ctgaattatc
     2821 acaggcagag atctcctggc gtgtgggtta tgaaaatgtc gatcactttg ccaaactgtt
     2881 tttgcgccat gtcggttgtt cgcccagcga ttaccgtaga caatttaaaa actgttttgc
     2941 ggaacaagaa atcctatctg aatttcctca accggtaagt cttgttggat aaatctggcg
     3001 gcgcagtttg ttaagaagct gcgccgcctg cagattattt gttctgatag tctttcagga
     3061 tctgccgacc tgctacgtaa atcataattt cgtctgtacc gccgccgata cgttcacaac
     3121 ggacatcacg ccagaagcgg gagacgcgcg cttcatcggt atagcccaga ccgcccatga
     3181 tttgaatcgc atcatcaatg acttccattg cggtacgtgc gcaatacagt tttgccagcg
     3241 ccgcgctggt gcgcagtgac tgatgctgat cggcttgcca tgccactttc agcaccatgt
     3301 tgcgcatgtt gtcgatctta atcgccatca gcgccagttt ttcctggatc atctggttat
     3361 gaccaatggg cttaccaaaa gcgatacgtt ggttggcata gcgggcggca tcttcaaagg
     3421 cgcattcggc aaagccggtg ctgcgcgcgg cgttgatcag gcgctccatc tcaaagttgt
     3481 acatcacatt gaggaaaccc attccttctt cgcccaccat gtcgctctct tcaacctcca
     3541 cgttgtcgag atagacttcg caggtgctga gcatatgcca gccgatttta tgcagcgggt
     3601 taatcttaat gccgggctta ctggagtcga cccaccacag ggtgaaggct tttttgggat
     3661 ctttcggttg cggatcgcgc gccaacacca gcatatacgg gtactctttc gcaccggtaa
     3721 taaaggtttt ttgtccgttg atgtagacct tgccgttttt acgcgtgtaa gtggtagtgg
     3781 cactgttgtt atctgagcca gcacctggtt ccgtcaacgc cagggcatag gcgggatcac
     3841 ccgtttccag ggtgctttct gccgttttac gtagctgctc tgcagaaccg aaacggcgca
     3901 tactgtgaat acattgaccg ttggtgatca aaaatgccgg agcaccgcat tttgacactt
     3961 ccatcagcgc cagcatttgg gtgacgtaat ccgcagggat accaccaaat tcttccggca
     4021 cgccaagcat ggaaataccg ttatccgcca gcgcccgcat aaactcacgc ggatatgtcc
     4081 cgttttgatc gcaggtgcgg aaatactctt ccggaaagtt agtcgtaatc agttcgcgaa
     4141 tactggccag cagcagttct tgttcttcag ttaaagaaaa atccatcatt atactcctgt
     4201 taatgagccg cttcaggcag gacaaaaccc atcgccgcat cgataaataa tctttcgtcc
     4261 atcagtttga gttctggcga aatcactggg gtgaagtcca ttttgtcgag aatatctttt
     4321 tgtaaatcga cgccaggggc gatttcgatt aaatgcaggc cgtcttcttt cagcgtgaat
     4381 acagcacgtt cggtgatgta acgaacatcc agtccccgtt cgagcgcgat ttttccgcta
     4441 aaagtaattt ccggtagttc ccgaataaat ttatttaccc gtccttcctg gacgatatgc
     4501 aatttgccat cggcaatttc tgtttttaaa ctgcccgcag ttaacgtgcc gcagaaaata
     4561 attttcttcg aggtggcact gatatcaata aatccaccgg tacccatgat tttgccattg
     4621 aatttatgca cgccgacgtt accgtgctgg tcgacttcag caaaactcaa ataacaaaca
     4681 tccagaccac caccgtgata aaaatcaaac tgggacgtca tatccagaat ggcgcgggta
     4741 ttcacgttcg cgccaaaggc gataccctgt gaagtaattc cgccaatcgg acctgtttct
     4801 accgtcagaa taaagtcatc agcacaacct tcttcccggg cgaccaggcc aataccgtca
     4861 gcaataccga cgccgacatt ccccaccgcg cctttacgca tttcgaataa tgcacgccgc
     4921 gcaactaatt tacgttggtt taggggcagg ctaagtttgg tgctgtcatc aagggtgaag
     4981 tcaccagaaa taaaacggtt aaccggcgca ccgccataca gttggctttg atccggatcg
     5041 accaccacaa tatccaccag ataacccgga atacggacag atttaggatg cagcgtggct
     5101 ttcttaacca ttttctgcac ctgcatcatc acaataccgc cgttattgtg caccgcctgg
     5161 gcaataacca atgcgtcgag atacatcacc tcatcttcaa aagtggcgta gccttcactg
     5221 tcgcaggtgg tagcgcgaat gaaggcgata tctggcgcaa tcgctttgta atagagatat
     5281 tctttgttat caaactcgac cagtttaatc aggtcttctt tcgtgacttc attcagtttg
     5341 ccgccttgct ggcgtggatc gacaaatgtc ccgatgccaa tatcactaat aataccaggc
     5401 tggtgggctg cggcggcgcg taaggtttgt gtaagtacac cttgtggata gttataagca
     5461 ataattttat tttgttctgc gagttcagaa atacgcggcg attgtcccca gtgaccacat
     5521 aatgcccatt tcaccagacc ttcttgcgcc agaggactaa taccgcggtc ggcgcgatcg
     5581 ccaagccctg ttggactaat aatcgataaa ttacgtggcg tttgagtctg tttatattta
     5641 tcagcaagag cagtaattaa cgtggtggct tccagaatac cgccgccagc gcctaacaca
     5701 caaagtgttg cttcgtcggg aatataattc accgcttcct gtgccgacag gaccggcact
     5761 cgtccattaa tacgaggtgg ttttacaggt ttcattgtta attcctgatt cagatgtgat
     5821 tttccctacg ccagatggag aattagcgca cagagactca cgaaatattt ttattaaata
     5881 cggttaaata tattttttaa gttcggcggg gagggtgttc ccgccgaaat attattgctt
     5941 atgcctggtg taaaatagtt aataccgtgc gtaaatcatt taccgagatt tgccctggtg
     6001 cagaggcttt ttttaccgca ccaaaagttg ccgccgagcc aaatacttca ccagccagac
     6061 gagaaattac gccagttttt gccatcgaca tcgtgatgat tggacgatcg gcatactgct
     6121 cctgcatctc cagggtcgcg gtaagcaacg tcagcacatc gctggtactt tgcggcatca
     6181 gcgcaatctt aggaatatcg gcgtcgaagg actgcatttt gcgcagacgg gcaatgattt
     6241 cttcggcttc cggcgtttta tggaagtcat ggttggacat gacaactttc acatcatgcg
     6301 cgtgggcgta ggcgacggtt tctttaacct gatcatcgcc ggtaaataac tccagatcga
     6361 tcatatcaac caggccgctg tcgatggctg cacgattgag agcaatataa gcctcggtgg
     6421 aaatcgcctg ctcgccgcct tctttggcac tgcggaaggt aaacagcagc ggtttttctg
     6481 gcatggtttc acggagaatt tttgccgccg ccatgacaga ctccacattg gagaggtcgg
     6541 caaagtggtc cacacgccat tccagaatat caaagtccgc ttcacgatag gcgagagctt
     6601 cggatttcac gcgggcgata tctttcgcca tcagcgagac gatgatttta ggtgcgcccg
     6661 taccaatgac gagatctttt acagttacgg ttttcatttt ttaccctttc tgcacgcggt
     6721 cagcctgtca ggcaccgaac cccatgacct gtttaacata ttccagagg
//
LOCUS       DAFATA010000162         6764 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFATA010000162 DAFATA010000000
VERSION     DAFATA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.164"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..392)
                     /locus_tag="LGJ20_004474"
     CDS             complement(<1..392)
                     /locus_tag="LGJ20_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2388464.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNHALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLP"
     gene            400..905
                     /gene="tnpA"
                     /locus_tag="LGJ20_004475"
                     /pseudo
     CDS             400..905
                     /gene="tnpA"
                     /locus_tag="LGJ20_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(923..1591)
                     /locus_tag="LGJ20_004476"
     CDS             complement(923..1591)
                     /locus_tag="LGJ20_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBK2388465.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1894..2487)
                     /gene="tehB"
                     /locus_tag="LGJ20_004477"
     CDS             complement(1894..2487)
                     /gene="tehB"
                     /locus_tag="LGJ20_004477"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBK2388466.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2484..3476)
                     /gene="tehA"
                     /locus_tag="LGJ20_004478"
     CDS             complement(2484..3476)
                     /gene="tehA"
                     /locus_tag="LGJ20_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBK2388467.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3600..4580
                     /locus_tag="LGJ20_004479"
     CDS             3600..4580
                     /locus_tag="LGJ20_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBK2388468.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4575..5111)
                     /gene="rimL"
                     /locus_tag="LGJ20_004480"
     CDS             complement(4575..5111)
                     /gene="rimL"
                     /locus_tag="LGJ20_004480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBK2388469.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5174..5398)
                     /locus_tag="LGJ20_004481"
     CDS             complement(5174..5398)
                     /locus_tag="LGJ20_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBK2388470.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5538..>6764)
                     /gene="opgD"
                     /locus_tag="LGJ20_004482"
                     /gene_synonym="mdoD"
     CDS             complement(5538..>6764)
                     /gene="opgD"
                     /locus_tag="LGJ20_004482"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBK2388471.1"
                     /translation="GVDTKQLEGQSDLGFAGFRVFKAPELARRDVVSFLGASYFRAVD
                     DTYQYGLSARGLAIDTYTDSKEEFPDFTAFWFDTVKPGATTFTVYALLDSASITGAYK
                     FTIHCEKSQVIMDVENHLYARKDIKQLGIAPMTSMFSCGTNERRMCDTIHPQIHDSDR
                     LSMWRGNGEWICRPLNNPQKLQFNAYTDNNPKGFGLLQLDRDFSHYQDIMGWYNKRPS
                     LWVEPRNKWGKGTIGLMEIPTTGETLDNIVCFWQPEKAVKAGDEFAFQYRLYWSAQPP
                     VHCPLARVMATRTGMGGFPEGWAPGEHYPEKWARRFAVDFVGGDLKAAAPKGIEPVIT
                     LSSGEAKQIEILYIEPIDGYRIQFDWYPTSDSTDPVDMRMYLRCQGDAISETWLYQYF
                     PPAPDKRQYVDDRVMS"
BASE COUNT         1812 a         1640 c         1558 g         1754 t
ORIGIN      
        1 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
       61 gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt
      121 ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta
      181 agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa
      241 gggatgtatt tattaccggc ctcatgattc tcattctgaa gcgccagagc atgattgaaa
      301 acgaaacgac atgcgcctgc gaagcgcctc atttcacgct cctgttgacc accgggtctt
      361 aactggaatt taaatgcctg taatcgtttc acaggccaaa tgatactttg gtctatgaat
      421 aaagaaaccg atatccgacg gggaagcatt gtgttttcct gatgcatgtt cacctggtct
      481 ttgtcaccag ataccgacgt cagatttttg actatgacgc gacagaaaaa ctacgcactt
      541 acttttcaaa tgtatgtgct gattttgaag ctgaactggt ttaaatggat ggcgaaccag
      601 atcacgtcca tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca
      661 gcctcaaagg cgtatcgagt aggttactgc gacgagatcg accagatatt gcagtcaggt
      721 attactacaa aggcgttttg tggagtcctg gctattttgc cagtagctgc ggaggtgcgc
      781 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag gtggaaaacc
      841 gcgccttata tccccgccct gaaggatggg gttttacggc gcaccggata aaaagcgcca
      901 tctaacgatg gcgcgtctgg aactactttt tgttaacgtc aaacatggtg gcatcggtcg
      961 ccatgtcatc aataacttgt ttaatatttt cgaaagccat tggcgtactt tcgttattca
     1021 ggtctttacc ttcgccctga cgaacgactt tgataaccgg tttattagtc gctgcatcaa
     1081 tcagctcacc ttcaaaatag aggcgagtat ccatggtacg gtggcctgta gccatttgcg
     1141 tccccgcaac cactaatgca acaggaacca cttcatagaa ttgcagccct tctttgctgg
     1201 tatctacacc ggtaatggcc ccacggaaaa tcagactacg cggcccagcg gtggtaacca
     1261 gtggtttacg ctgcgctatc gcttctttca tttcggtgtt ggtatagttc aaaattttat
     1321 ccagaacttt ctgccctacc tgggtagaag gttttggtac cggataataa gtgattgggt
     1381 tccagacgat gctgtcatat ttgctttgat caaaactcgg gtctacccaa cgtaaaacag
     1441 gtttacctgt tgctgaggtt gtttctttta aatcagagta attgtttaaa aaaccagaat
     1501 atttatctgg ctgggtgatt ttagaggcac aaccagacag agccaacaag ccgcataaag
     1561 ctgcaacttt cgcaaatgat gtggtacgca tgaatagttt cctgagatga ttatattact
     1621 ttctaagtta tagcaaaaag cgccgcttcg tgttgtggca aaaaaagtgt tggtgaaaat
     1681 aaatttttca gccccttttc agacgacata gtcaatgccg aaaaaggggc taagtcaatc
     1741 atcagtagtt aaatttcagt aagttaagct gctaaaaaac ttcgactact gtgattaatt
     1801 tcacatccct tcgataaaaa atatgcttac cgaaaagaag aacagtaaat catctcagcg
     1861 tggggaattt tcacgcgcct tattcttagc gggtcatttt ttacgtgcca gcatcgtggc
     1921 gaaacgcagt ttaatacgat taccgttggc gtcggtgcgg tgcagctcgc cgacgtcttc
     1981 attgtatttc accctctccc agccttcgta atatcgacgt aattctccct ctttgaaggc
     2041 aaacgggaag ccgacggtac atggataatc agcggtatcc atcgccgcca caatcaggtt
     2101 gtaaccacca ggtttagtgc aacgttgcat attgacaatc agccctggga tggttttagc
     2161 ctcaaggaac atcagtacca cagtcgaaag aataaaatcg tactgtctat caaatgtgag
     2221 gttattcaga tcaacgactc gggtgtgtaa attatccaga ttttcaatgg atttaatgcg
     2281 ctcgacgttg gcgatactca tggcattttt atcccatgcg tcaacatcat aaccattggc
     2341 tgccaggtaa agactgttac gaccattgcc acagcccaga tccagcgttt tacccggttt
     2401 aaccactttc accgcttcca gtacttcaga gtgtgtgcgg gttaattcat atttatcagt
     2461 aaaatagttt tcgtcacgaa tgatcattct ttgtcctctg ctttcattaa aacggcgcgc
     2521 tcggttctga ctaacaattt tccctgcatc agaagcgcaa aagtacggat gagcagtatt
     2581 gcaataataa aattggtaaa gataaacagc ggcaccgcca gcgtatggaa aaatccatta
     2641 tcgctgccac tccccagatg caaaccggtg gttgccagtg cagatacgcc gaacgagaaa
     2701 ctccagaatg aagcattaaa tggctgggag agataccatg gcatcagacg tagcataaac
     2761 agcagttgca gcagtccata accgaaaagc attttcgcca gcgtgtcacc ctcgccgccg
     2821 ttgacgctca gccaggcact acaagccacc agcgcaggag cgagctgaat gccgagtgat
     2881 gtccgcagtg ccgtgggtaa ttctcccgaa ctgcgcagac gctgcaagat caccggttca
     2941 aggcttagcc atgagaaaac gcctgcgcct aaaaacacca gaccggcgtc ggtgtagccc
     3001 aacgcaccac aggccattgc gctgataaag ttgttggcaa ctgtcggcag atacagtcca
     3061 ggcgtggtag cttcttcagg gtgagatccg cgccataatc ccgcagtttg ccaggcggca
     3121 taagccaact gaaccacgac accaaaactg aacaggcaca ccgccagtgg gcgaaaccac
     3181 ggaacaaaac caatcgccac cagcatcgtc gttgccggaa acaaactcac aaaactgctc
     3241 agcactggat ggcgaacttc cgccagcacg ctatgcggaa agcgtatgag tcgggtaata
     3301 aatgcgctag ttaataatcc ccagatgatc atcgccagaa tcaccagccc atcccctaac
     3361 cagtggctga cctgccaaac ctggctggca tagcgccagg caaatcccat ccctatcgtc
     3421 cccaacacaa taccaaagta gcctgccggc aaattgagca ctttatcgct ctgcatcttc
     3481 ttactcattt tgtttaattt ataaagtata tttgaaatgc attttatgga attttctcga
     3541 gcatagccag agccgcagaa tttgctacgg ttatagtaag tcacctgtgg gaaaacgcta
     3601 tgcgcaaata tcgtctaagt gaagaacagc gagcctttag ttatcaggaa gatggcacta
     3661 aaaaaaatgt gttattacgg cagattatcg ccattagcga ttttaacgat gtgatagcag
     3721 gaaccgctgg cggctggatc gatcgcgaaa cggtgttagc acaggaagga aattgctgga
     3781 tttacgacca gaatgctatt gcgttcggcg gaacggtgat ttccggtaat acgcgcatca
     3841 ccggcaccag cgtactgtgg ggagaggttt acgcaacgga taatgtctgg atcgataata
     3901 gtgaaatcag ccagggtgca tatataagtg acagtgtcac cattcatgac tcactggttt
     3961 gcgggcaatg tagaatattt ggtcacgcct taattaatca acattctatg atcgtcgcag
     4021 cacaaggctt aacgcctgac catcaactcc tgctacaaat ttatgatcgc gcaagggtta
     4081 gcgcctcgcg tattgtccac caggcacaaa tttatggcga tgccgtcatc cggtatgctt
     4141 ttattgaaca tcgtgccgaa gtttttgatt ttgccagtat tgaaggtaat gaagagaaca
     4201 acgtctggct atgcgattgc gctaaagtct acggtcacgc gcaggtgaaa gctggcatag
     4261 aagaagatgc cattccaacg attcattaca gttcgcaggt ggcggaatat gccattgtgg
     4321 aaggtaattg tgtgttaaaa catcatgtct tgattggcgg aaatgccgtg gtacgtggtg
     4381 gaccgattct gctcgacgag cacgttgtaa tacaaggtga gagccgtatt acaggtgcgg
     4441 tgataattga aaaccatgtg gagttaaccg accacgccgt cgttgaagcc tttgatggcg
     4501 acacggttca tgtccgtggt ccaaaagtca ttaacggcga ggagcgcatt acgcgaacgc
     4561 cgctggcagg gttattatga atcgataata cgcgcgtata agttcacatc atcataggca
     4621 tcattcagga actcagcctg tttcaggcaa ccttcaagga taaaaccatt gcgcaaagcg
     4681 acctggttgc tttgcggatt gtccacccga catttgatca cgaagcgtct aagttcacca
     4741 gactgggcgt aatgatgaat caatgcctgc agcgcctgag aaatgatccc ctgcccctga
     4801 tgagattcgt ccagccagta gcctatttca gcggttttat tcagtggttc aatacgatta
     4861 aacgagataa cgccgataag ttcatcttct ttgaaaatca tgaacatttt ggcatagccg
     4921 cgttgatgca acatcacatt accctgcacc gtttttcgcg tgtcctcttc actctgaacg
     4981 aactgcggcc agtttagcga ctgctgtaac caggttttat ttttacagat taactgataa
     5041 agaggagtga cgtgattttc tgcaacagca tgtaattcaa gtgattcgct tacttttatc
     5101 gtttcagtca tcttatctcc agtcaatcca tgcagttgtt aaaaaagccg cctggctggc
     5161 ggcttaggaa agtttatacc tctttgacgc tctcctgctg caggattttg agcatctcat
     5221 cttttaactg tagcttttgt tttttcatgc ggaccacttc cgcattgtac cctcgaccgt
     5281 cggaaccttc ctttctggca atttcatgat caagtttatt gtgtttatcg aacaaggaca
     5341 taaagcgagg attttcgttt ttcagacggg atattaaatc tcgatattct ggaaacatac
     5401 gcactccctg ttagtggctg acaaggtgta tctgaaatac acaccatcag attactcatt
     5461 taacttaggg aaaaatgcga gcgggatcgt gtttaaccag acggcacctc ccgaagaagg
     5521 tgccgaagaa aaaacgatta actcatcacg cggtcgtcaa catactgacg tttatccggc
     5581 gctggcggga aatactgata cagccatgtt tcactgatag cgtcgccctg acaacgcaga
     5641 tacatccgca tatcgaccgg atcagtggag tccgaagtcg gataccagtc aaactgaata
     5701 cgataaccat caatgggttc aatatagaga atttcgattt gcttcgcttc cccactggaa
     5761 agcgtaatca ccggctcaat gccttttggt gcggcagctt tcagatcacc accaacgaaa
     5821 tcgacggcaa aacgacgcgc ccatttttcg ggatagtgtt cacctggcgc ccaaccttcc
     5881 gggaaaccgc ccatgccggt acgcgtcgcc ataacgcgcg ctaatgggca atgaacaggc
     5941 ggttgcgcac tccagtacag acgatactgg aatgcaaact catcacccgc ctttacagct
     6001 ttttctggct gccagaagca gacaatgtta tccagcgttt cgcccgttgt tgggatttcc
     6061 atcaggccga tggtgccctt accccacttg ttacgcggtt ccacccacag acttgggcgt
     6121 ttgttatacc agcccataat gtcctgataa tgggagaagt cacgatccag ttgcaataaa
     6181 ccaaaccctt tcgggttgtt gtcggtgtaa gcattgaact gcaatttttg cggattattc
     6241 agcggacggc aaatccactc gccgttgccc cgccacatgg acagacgatc agagtcatga
     6301 atttgcggat gaatggtgtc gcacatccga cgttcattag taccgcagct gaacatactg
     6361 gtcatcggcg caatgcccag ctgtttaatg tctttgcgcg catacaggtg attttccaca
     6421 tccataatca cctgactttt ctcacaatgg atagtgaact tataggcacc agtaatgctg
     6481 gcgctatcga gcaacgcata aacggtaaag gtagttgccc ccggttttac cgtatcaaac
     6541 cagaaggcgg taaagtcggg gaactcttct ttactgtcgg tgtaagtgtc gatcgccagg
     6601 ccgcgggccg acaaaccgta ttgatatgta tcatcaacgg cgcggaaata actcgcgccg
     6661 agaaatgata ctacatcacg gcgcgccagt tcgggggctt taaacacgcg aaaaccggca
     6721 aagccgagat cgctttgccc ttctaattgt tttgtatcaa cacc
//
LOCUS       DAFATA010000163         6748 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFATA010000163 DAFATA010000000
VERSION     DAFATA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.165"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..118)
                     /gene="fxsA"
                     /locus_tag="LGJ20_004483"
     CDS             complement(<1..118)
                     /gene="fxsA"
                     /locus_tag="LGJ20_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="F exclusion of bacteriophage T7; overproduction of
                     this protein in Escherichia coli inhibits the F
                     plasmid-mediated exclusion of bacteriophage T7; interacts
                     with the F plasmid-encoded PifA protein; inner membrane
                     protein; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBK2388472.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFT"
     gene            455..1891
                     /gene="aspA"
                     /locus_tag="LGJ20_004484"
     CDS             455..1891
                     /gene="aspA"
                     /locus_tag="LGJ20_004484"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBK2388473.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            2009..3310
                     /gene="dcuA"
                     /locus_tag="LGJ20_004485"
     CDS             2009..3310
                     /gene="dcuA"
                     /locus_tag="LGJ20_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBK2388474.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            3426..3764
                     /gene="cutA"
                     /locus_tag="LGJ20_004486"
     CDS             3426..3764
                     /gene="cutA"
                     /locus_tag="LGJ20_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBK2388475.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            3740..5437
                     /gene="dsbD"
                     /locus_tag="LGJ20_004487"
     CDS             3740..5437
                     /gene="dsbD"
                     /locus_tag="LGJ20_004487"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBK2388476.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            5474..6049
                     /locus_tag="LGJ20_004488"
     CDS             5474..6049
                     /locus_tag="LGJ20_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2388477.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            6156..6231
                     /locus_tag="LGJ20_004489"
     tRNA            6156..6231
                     /locus_tag="LGJ20_004489"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6189..6191,aa:Phe,seq:gaa)
BASE COUNT         1680 a         1711 c         1674 g         1683 t
ORIGIN      
        1 acgtgaatat aaccagcacg agggtcagta atacccccaa tacatgggca acctgaataa
       61 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
      121 gtttctcctg taatagcagc cggtaaaacc cggctacctg aatgggttgc gaatcgcgtt
      181 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
      241 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
      301 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
      361 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
      421 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
      481 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
      541 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
      601 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
      661 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
      721 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
      781 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
      841 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
      901 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
      961 actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg
     1021 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     1081 cctgctgaaa gaagaagtga aaaatatcca acgtaccgct gaactgctgc tggaagttaa
     1141 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     1201 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     1261 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     1321 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     1381 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     1441 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     1501 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggagc cggtcattgg
     1561 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     1621 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     1681 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     1741 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     1801 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     1861 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaagaaggc
     1921 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca acctaatatc
     1981 aacttgttaa aaaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     2041 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     2101 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     2161 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     2221 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     2281 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatat ctctggcgac
     2341 actgccagtt atcgctgaag ttgcgaagga acaaggcgtc aaaccttgcc gtccgctgtc
     2401 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     2461 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     2521 catcccgtcc accctgctgg cggttctggt gatgtccttt ctggtcacta tgctgttcaa
     2581 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggcc tggttgaact
     2641 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     2701 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     2761 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     2821 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     2881 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     2941 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     3001 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     3061 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     3121 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     3181 acagatggat gacacgggta caacccgtat cggtaaattc gtcttcaacc atccgttctt
     3241 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     3301 catgctgtaa tgaccaatcg cggggcgttc aagccccgct ttctttcccg ccgactaaca
     3361 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     3421 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     3481 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     3541 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     3601 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     3661 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     3721 gagacacaga ttacctctca tggctcaacg catctttacg ctgatcctgc tactttgcag
     3781 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     3841 tcaagccttt acttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     3901 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     3961 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     4021 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
     4081 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     4141 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     4201 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     4261 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     4321 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     4381 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
     4441 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
     4501 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
     4561 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
     4621 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
     4681 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
     4741 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
     4801 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
     4861 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
     4921 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
     4981 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
     5041 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
     5101 aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
     5161 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
     5221 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
     5281 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
     5341 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
     5401 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
     5461 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
     5521 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
     5581 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
     5641 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
     5701 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
     5761 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
     5821 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacact
     5881 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
     5941 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
     6001 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
     6061 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
     6121 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
     6181 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
     6241 aaacggacct ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
     6301 ctccaaatca attctcccga actcaaccag attctcccct catcaccagc aatttgcggg
     6361 catatcccaa ttcgggaaaa tttgttcctg agcgatagca ctaactgacg tgaaatgtcg
     6421 tgcggccccg tgatgttgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgcatccata
     6481 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
     6541 tgtatcagtc cgcttgctca tgcaaagact aacaatccat taaaatagta agcgctccgg
     6601 acaattttcc atggattatt ttctgaacat ttttctttgg caaagatgat gaattttgat
     6661 ggtaaggaat attacttctg gttctcagta aaatcctttc gtaatactat gtaatcaaga
     6721 agtttatggc tggtaaaaat aacgtctt
//
LOCUS       DAFATA010000164         6745 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFATA010000164 DAFATA010000000
VERSION     DAFATA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.166"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..199)
                     /gene="tuf"
                     /locus_tag="LGJ20_004490"
     CDS             complement(<1..199)
                     /gene="tuf"
                     /locus_tag="LGJ20_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK2388478.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTS"
     gene            complement(270..2384)
                     /gene="fusA"
                     /locus_tag="LGJ20_004491"
     CDS             complement(270..2384)
                     /gene="fusA"
                     /locus_tag="LGJ20_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK2388479.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2481..2951)
                     /gene="rpsG"
                     /locus_tag="LGJ20_004492"
     CDS             complement(2481..2951)
                     /gene="rpsG"
                     /locus_tag="LGJ20_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK2388480.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3048..3422)
                     /gene="rpsL"
                     /locus_tag="LGJ20_004493"
     CDS             complement(3048..3422)
                     /gene="rpsL"
                     /locus_tag="LGJ20_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK2388481.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3548..3835)
                     /gene="tusB"
                     /locus_tag="LGJ20_004494"
     CDS             complement(3548..3835)
                     /gene="tusB"
                     /locus_tag="LGJ20_004494"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBK2388482.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3843..4202)
                     /gene="tusC"
                     /locus_tag="LGJ20_004495"
     CDS             complement(3843..4202)
                     /gene="tusC"
                     /locus_tag="LGJ20_004495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBK2388483.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4202..4588)
                     /gene="tusD"
                     /locus_tag="LGJ20_004496"
     CDS             complement(4202..4588)
                     /gene="tusD"
                     /locus_tag="LGJ20_004496"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBK2388484.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4588..5310)
                     /locus_tag="LGJ20_004497"
     CDS             complement(4588..5310)
                     /locus_tag="LGJ20_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2388485.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQSDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSNVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFLGQDK"
     gene            complement(5477..6289)
                     /gene="fkpA"
                     /locus_tag="LGJ20_004498"
     CDS             complement(5477..6289)
                     /gene="fkpA"
                     /locus_tag="LGJ20_004498"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK2388486.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6510..6728
                     /locus_tag="LGJ20_004499"
     CDS             6510..6728
                     /locus_tag="LGJ20_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBK2388487.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
BASE COUNT         1672 a         1725 c         1612 g         1736 t
ORIGIN      
        1 gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga
       61 atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca gcggtcagag
      121 tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt tttgtacgtt
      181 caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct tcaggagaga
      241 gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat tacggcctga
      301 gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga tgcacgacct
      361 ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg tacttcagcg
      421 tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg acgacggctc
      481 aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac cttcatgatc
      541 ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat agaagcagcc
      601 agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg cagacgaata
      661 cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc ctggatacct
      721 ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc gttgatgaac
      781 tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac aacatgacca
      841 tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt aactttctgg
      901 cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac gttgaattca
      961 cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc gataatggtc
     1021 tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc cagacggccc
     1081 agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc gatggagatt
     1141 accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc acacagggtg
     1201 tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc gcgaacttct
     1261 ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca
     1321 gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta aacacggaag
     1381 aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc agagaacggc
     1441 tcatcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag gataccgttg
     1501 atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag catcgcctga
     1561 acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt gttcagaacg
     1621 cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc caggtatttt
     1681 tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg ccattcgtta
     1741 gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc ctggtcagcg
     1801 tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa atgttcttca
     1861 gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt gatctggtta
     1921 acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc aatgcgcgga
     1981 actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac accaccaact
     2041 gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac ttcgattgtg
     2101 aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc
     2161 ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg ttcctgctcc
     2221 tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt atggtttaca
     2281 ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat gtgcgcactg
     2341 ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt cctcgtttat
     2401 cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc gcccgcctgg
     2461 tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc
     2521 atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc agcatcagaa
     2581 agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc agcttcaacg
     2641 atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg tacctgataa
     2701 gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt ttcgagagct
     2761 acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc cagcgcgctg
     2821 tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt tacaaattta
     2881 gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc aatgacgcga
     2941 cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc tacgagttta
     3001 gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta agccttagga
     3061 cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga gcagtcaagc
     3121 gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc gccacggatc
     3181 aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga agtcacttcg
     3241 aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg ttttttagga
     3301 gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc
     3361 acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc
     3421 attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac gtcctcacac
     3481 aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat
     3541 cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg aaatcagtat
     3601 agtcaatggg aataatgtcg ttcgaaattt gaccagtcaa accgcgggca ataaggtctt
     3661 cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag cggttaccgt
     3721 caactgcggc agttacacca tcttgcaata atagcagttc gtcgccctca ctgagcagac
     3781 gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taatgtgtgc agcataaacg
     3841 cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc atctgcttcg
     3901 agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc gcgcagtgaa
     3961 gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa agtggcaatg
     4021 taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa aacgccatca
     4081 gcgataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa agcatctaaa
     4141 ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc aattcgtttc
     4201 atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcc ccaagtccgc
     4261 ttaaggtaaa tcctggctga aggtttgacg aagccagccc cagccttcct gcttcagttt
     4321 catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc acaccatgtt
     4381 gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc ggtgaggtca
     4441 attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac tcatggcctt
     4501 cggctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta ccgtatgctg
     4561 gcccggtcac cacgatggca aaacgcatta cttatcttgc cccaggaagt cgccactttt
     4621 gaactggcga atatagagat aaacagtatg tttggaaata ttcagacgat cagcaacctg
     4681 attaatcgcg tcttttatgt cgaatatccc tttctcgtag agattcaata cgatctggcg
     4741 atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg tgaactccag
     4801 cgtttgggta accagatctt ctacagagga agcaaagttg acatttgagc cgacatccgg
     4861 cgtctctggt ggcacgaagg tgctcataat ctgcgagaaa ggaacatcga ggttcatatt
     4921 aatgcacagc aggccaatta cacgctgttc gcgattacga atggcgatgg tcagcgactt
     4981 cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc tatccgctcc
     5041 cgtcatatcg tgaagcatac gtagcgccag gtcggtaatt ggcgaaccaa tcttccggcc
     5101 tgtatgttca ccgttagcaa tacgaatggc tgaacatttt aaatcctgca aagagtgcaa
     5161 aacgatttca cagtgggagc caatgagcat cgctaacccg tccaccaccg cttcgtagga
     5221 tttcagaata tcaaaatcgc tctggtcgaa aggacgttga tccagtaaat ccaactcact
     5281 ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc aggagcctgt
     5341 cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat acagcggagg
     5401 ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg cctggtcagg
     5461 cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca gcttccggct
     5521 ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac accagggtag
     5581 aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt ggaataacca
     5641 gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc gggataacac
     5701 cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac tctttaccgt
     5761 cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt gcttcgcctt
     5821 taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca cctttctctt
     5881 tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg tctttttcca
     5941 tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc tgttcgatct
     6001 cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg atcagctgat
     6061 ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc atgtaacgac
     6121 ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac gctgctttgc
     6181 tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt gcatgcaggg
     6241 caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc catatctcca
     6301 ggatcggggc atctcacccc agggttaact attatcagaa gggtactata aagcgttgtc
     6361 gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct ttttgtttaa
     6421 attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa gtagaatccg
     6481 cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa gcacgcctgg
     6541 cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg aacgtcacgg
     6601 tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg ctgaccgaga
     6661 agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg ccaccgccac
     6721 attattgagg cgtaaaaaaa gcggg
//
LOCUS       DAFATA010000165         6674 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFATA010000165 DAFATA010000000
VERSION     DAFATA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.167"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..810
                     /locus_tag="LGJ20_004500"
     CDS             <1..810
                     /locus_tag="LGJ20_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HBK2388488.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            877..5085
                     /locus_tag="LGJ20_004501"
     CDS             877..5085
                     /locus_tag="LGJ20_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2388489.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLAWLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGD
                     VIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPG
                     QQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLY
                     QLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGH
                     AGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESV
                     GIGAGVDASVMTLACRTWQECWVN"
     gene            5070..5267
                     /locus_tag="LGJ20_004502"
                     /pseudo
     CDS             5070..5267
                     /locus_tag="LGJ20_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            5283..5492
                     /locus_tag="LGJ20_004503"
     CDS             5283..5492
                     /locus_tag="LGJ20_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388490.1"
                     /translation="MNEAKEKDLGTYKKSTLKTEKITRGLFSNDEITLIYFSEYSKRI
                     VQEVFVFNVEDKKVKLKGYRYDSIN"
     gene            complement(5514..5633)
                     /locus_tag="LGJ20_004504"
                     /pseudo
     CDS             complement(5514..5633)
                     /locus_tag="LGJ20_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            6000..6674
                     /locus_tag="LGJ20_004505"
                     /pseudo
     CDS             6000..6674
                     /locus_tag="LGJ20_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1738 a         1613 c         1996 g         1327 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt
      961 ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg
     1021 gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc
     1081 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg
     1141 gtgggagtgt ttggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgcgat
     1201 gatgcactgg tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg
     1261 ggggaggcgg tgtacagccg cagcgagtca atgtggctgg tgcgcggtgg taaggcagcg
     1321 cagccggacg gccacacgct ggcgtggctg tggggggcgc tgccgccgga tatccggtta
     1381 agcccgcatc tttacctggc gaccaacagc gcacaggggc cgtggtggat actggggtgg
     1441 tcagagcggg tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg
     1501 cttaccgggc tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc
     1561 gacctggccg gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta
     1621 ctgaccacac aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac
     1681 agttcccgcc ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc
     1741 cccgacaggg gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa
     1801 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
     1861 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     1921 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
     1981 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
     2041 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2101 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
     2161 tatgacgcgg caggaaggct aacggcgcag acggacgcgg cgggacggcg gacagagtac
     2221 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2281 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
     2341 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg cagtggggac
     2401 gtcatccggt atgcttatga taatccgcac agtgaattac cggccacgac aacagatgcg
     2461 acgggcagca cccggcagat gacctggagc cgctacgggc agttgctggc gttcaccgac
     2521 tgctcgggct accagacccg atatgaatac gaccgcttcg gccagatgac ggcggtccac
     2581 cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt aacctcggtg
     2641 aaagacgcac agggccgtga aacgcggtat gaatacaacg ccgcaggcga cctgactgcc
     2701 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     2761 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
     2821 agcctgacca acgagaacgg cagccacagc gtcttcagtt acgatgcgct ggaccggctg
     2881 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
     2941 ctcacacaga gtgaggatga ggggcttgtc accctctggt actacgatga atcggaccgt
     3001 atcactcacc gcacggtgaa cggcgaaccg gcagagcagt ggcagtatga tgaccacggc
     3061 tggctgacag acatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
     3121 gacgataagg gccgcctgac cggcgaacgg cagacggtgg agaacccgga gacgggggaa
     3181 ctgctgtggc agcatgagac aacacacgcc tacaacgaac aggggctggc aaaccgcgtc
     3241 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta tctggcgggc
     3301 atgaagcttg gcgacacgcc gctgctggag tacacgcgcg accgcatgca ccgggagacg
     3361 gtgcgcagct ttggcagcat ggcaggcagt aatgccgcat ataaactgac cagcacatac
     3421 acccccgcag gccagttaca gagccagcac ctgaacagcc tggtgtatga ccgtgactac
     3481 gggtggaatg acaacggcga cctggtgcgc atcagcggcc cgcgacagac gcgggaatac
     3541 ggctacagcg ccacgggcag gctggagagc gtgcgtaccc tcgcaccgga cctggacatc
     3601 cgcatcccgt atgccaccga cccggcggga aaccgcctgc cggacccgga gctgcacccg
     3661 gacagtaccc tcacggcgtg gccggataac cgcatcgcag aggatgcgca ctatgtttac
     3721 cactatgatg aatacggcag actgacggag aagacggacc ttatcccggc gggtgttatc
     3781 cggacggacg acgagcggac ccaccactac cactacgaca gccagcaccg tctggtgttc
     3841 tacacgcgga tacagcatgg cgagccactg gtcgagagcc gctacctcta cgacccgctg
     3901 ggacggcgaa tggcgaaacg ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg
     3961 ctgtcgcgta aaccggagat gacgtggtac gggtgggacg gcgaccggct gacgacggta
     4021 cagaccgaca ccacgcgtat ccagacggta tatcagccgg ggagcttcgc gccgctcatc
     4081 cgcatcgaaa cagacaacgg cgagcgggag aaagcgcagc gccgcagcct ggcggagaag
     4141 ctgcagcagg aagggagcga ggacgggcac ggcgtggtat ttccggctga actggtgcgg
     4201 ctgctggaca ggctggagga agaaatccgg gcagaccgcg tgagcagtga aagccgggcg
     4261 tggcttgcgc agtgcgggct gacggtggag caactggcca gacaggtgga gccggaatac
     4321 acaccggcgc gaaaagttca tttttaccac tgcgaccacc ggggcctgcc gctggcgctc
     4381 atcagcgaag acggcaatac ggcgtggcgc ggggagtatg atgaatgggg caaccagctt
     4441 aatgaagaga acccgtatta cctgcaccag ccataccgtc tgccggggca gcagcatgat
     4501 gaggaatcag ggctgtacta taaccggaac cggtactatg acccgctaca ggggaggtat
     4561 attacacaag accccattgg gctggcgggg ggatggaacc tgtataatta cccactgaat
     4621 ccgataataa ggatggatcc tttgggtttg tataatttat atcaattatt atatgatgtt
     4681 tggcatgatg attcatatgg aacatcatca attgatatta ctggcagtgg agatctaata
     4741 tcattaggtg gtcatgcagg acttggcgtt gcgtttgcta aaaagaaagg tgaaatgtta
     4801 tctgatattt gtatttatgc tacagcatgc ggacatgcag gaattggtgg tgggataaat
     4861 gcggctatca catattctga gaccaagtct ttacctacat cgggagtcag caattcagta
     4921 ggtgtaacgg ttggcggcgg agttgggggg cattttgcgt atacttatgt agtggatgtt
     4981 gataatccag aatcatcgac agaatctgtt ggtatcggtg caggtgttga cgcttcagtt
     5041 atgactctgg cttgtagaac gtggcaagaa tgctgggtca attaacagga ggaaatatga
     5101 aaaaatatat atttttagtt ttatttttat tgtgtggttg ttctccagtg gacatagagc
     5161 cagctagaca agaagttaat atctttcgtg gtttatatca gacagggaaa tacagtgata
     5221 tatataaaat cacttcaagt gattttcaga ctgccacatc tgaaaaaaaa tttatcgaca
     5281 taatgaacga agcaaaagaa aaagatcttg ggacttataa aaaatcaacc cttaaaactg
     5341 aaaaaatcac tcgcggatta ttttcaaacg atgaaattac attgatatat ttctcagaat
     5401 attcaaaaag aattgtccaa gaggtttttg tgtttaatgt tgaagacaaa aaagtaaaat
     5461 taaaaggtta taggtatgat tcgattaatt aaattttttg atgatgactt caattagtag
     5521 aacatgtatt tttcgataaa agcaccgatg actttttcat gaagttctac agagcgcgag
     5581 aaacagatgg ttctgcgagt cagacgtttg attcaggttc tcaggggcag attcagggaa
     5641 agatcacagg gaaagatcat cggtgtgccg taggcacaaa gatgcaaaac gggacgtttt
     5701 gcatgtaata agcataagct tgtgaagaga tggtggcagg cccaccggga tctgctgtcg
     5761 gaagtcggtc ccaaacaacc ccggctgctg tggtaaagag ccatggtagt gagcgcaggg
     5821 ggaacgatca gacgagatct gatcctatgt gagattaggg aaagatcatc aaaagttcac
     5881 tttttgtact aaataattcg cattttatgt ttaaaaattg agatattact cattacctga
     5941 agctgttttt tattgcttat acatgatcaa atactcctta cataattaag gagaacaaaa
     6001 tggaacttaa aaaattgatg gaacatattt ctattatccc cgattacaga caagcctgga
     6061 aagtggaaca taaattatcg gatattctac tgttgactat ttgtgccgtt atttctggtg
     6121 cagaaggctg ggaagatata gaggattttg gggaaacaca tcccgactca acaatgcata
     6181 gtctggtcct cggacagatc aagacggatg agaaatccaa tgagattaca gccattcctg
     6241 aacttcttaa catgatggat attaaaggaa aaatcatcac aactgatgcg atgggctgcc
     6301 agaaagatat tgcagaaaag atacaaaaac agggaggtga ttatttattc gcggtaaaag
     6361 gaaaccaggg gcggctaaat aaagcctttg aggaaaaatt tccgctgaaa gaattaaata
     6421 atccagagca tgacagttac gcaatgagtg aaaagagtca cggcagagaa gaaatccgtc
     6481 ttcatattgt ttgcgatgtc cctgatgaac ttattgattt cacgtttgaa tggaaagggc
     6541 tgaagaaatt atgcgtggca gtctcctttc ggtctataat agcagaacaa aagaaagagc
     6601 cagaaattga cggtcagata ttatatcagt tctgctgatt taaccgcaga gaagttcgcc
     6661 acagcaatcc gaaa
//
LOCUS       DAFATA010000166         6610 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFATA010000166 DAFATA010000000
VERSION     DAFATA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.168"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..448)
                     /gene="kdgR"
                     /locus_tag="LGJ20_004506"
     CDS             complement(<1..448)
                     /gene="kdgR"
                     /locus_tag="LGJ20_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBK2388491.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGK"
     gene            625..1998
                     /locus_tag="LGJ20_004507"
     CDS             625..1998
                     /locus_tag="LGJ20_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388492.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILTVIAACVSGLRI
                     TQPRSRA"
     gene            complement(2044..2925)
                     /gene="htpX"
                     /locus_tag="LGJ20_004508"
     CDS             complement(2044..2925)
                     /gene="htpX"
                     /locus_tag="LGJ20_004508"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBK2388493.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(3117..5165)
                     /gene="prc"
                     /locus_tag="LGJ20_004509"
     CDS             complement(3117..5165)
                     /gene="prc"
                     /locus_tag="LGJ20_004509"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBK2388494.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(5185..5883)
                     /gene="proQ"
                     /locus_tag="LGJ20_004510"
     CDS             complement(5185..5883)
                     /gene="proQ"
                     /locus_tag="LGJ20_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBK2388495.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(5980..6477)
                     /gene="msrC"
                     /locus_tag="LGJ20_004511"
     CDS             complement(5980..6477)
                     /gene="msrC"
                     /locus_tag="LGJ20_004511"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBK2388496.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
BASE COUNT         1497 a         1780 c         1536 g         1797 t
ORIGIN      
        1 ccttaccaat cgcggtgctg tacagcggat tacgacgccc aatccgtgaa tacatgcgca
       61 aattgtacat agagtcaatt ttgtgaatgt aaacaatact gtcttcgtcc agtgcgccga
      121 ggtggatagt ttctttggtc aggcgggaga tctcacgcat ctggatatct gcgctacgaa
      181 ttaaatcgac gttttgtaac gcgcgagcgc ccagttcaaa caatttcagg gtcagggaat
      241 atttctccga ctccccttcc tgcgccacat aacctaaggt tttcatggtc tgtaaaaagc
      301 gataaacggt gctttttgac atcatgacgc gctgcgacag ctcggttatc cctatttcgc
      361 gctcttcacc cagcgcctgc aaaatgccaa aaacttttag cacggaagat acagaatcag
      421 gctgtttatc cagatctgcg ttagccattt atcacctcat tgcgagtgtt ttataaaaat
      481 cagaactgtt ttttattata atttcgcacc agggtggtcg caatccatct tttgccggtt
      541 agttacaatt ctgcgacatc caccgtgaat atcagtgcta gaatcatacc cctgttgatt
      601 attcaccaaa gatataaaat tcctatgcca aaagttcagg ccgacggcct gccattgccc
      661 cagcgatacg gtgcgatatt aaccattgtg attggtattt cgatggctgt ccttgacggc
      721 gcaatcgcca acgtcgccct gccaacaatc gccacggacc ttcatgccac gccagccagt
      781 tccatctggg tagtgaacgc ctatcaaatc gccattgtca tctccctgct ctcgttttcg
      841 tttctgggcg atatgtttgg ctatcgacgt atttataaat gcggtctggt cgtttttctg
      901 ttgtcttcac tgttctgcgc cctttctgat tcgctgcaaa tgctcaccct tgcgcgtgtc
      961 atacaaggtt tcggcggtgc agcgttgatg agcgttaata ccgcacttat ccgcctgatc
     1021 tatccacaac gttttctggg gagagggatg ggcataaact cgtttattgt tgccgtttct
     1081 tctgctgccg ggccgacaat tgctgcagca atcctctcca tcgcatcctg gaaatggtta
     1141 tttttaatca acgtaccgtt aggtattatc gccctgcttc tggcgatgcg ttttctgcca
     1201 cccaatggtt ctcgcgccag taaaccccgt ttcgacctgc ccagcgccgt gatgaacgcg
     1261 ttaaccttcg gcctgcttat cactgcgttg agtggtttcg ctcaggggca atcgctgacg
     1321 ttaattgctg cggaactggt ggtaatggtt gttgttggta ttttctttat tcgccgccag
     1381 ctttctcttc ccgtaccgct gctaccggtg gatttactgc gtatcccgct gttttcactt
     1441 tctatttgca catctgtttg ctctttctgc gcacaaatgc tggcaatggt ttccctgccc
     1501 ttttacctgc aaaccgtgct cgggcgtagt gaagtcgaaa caggtttact tctgacaccg
     1561 tggccgttag caacgatggt gatggctccg ttggcaggct atttgattga acgcgtacat
     1621 gcaggattgc tgggtgctct agggttgttc atcatggctg cggggctttt ttccctggtt
     1681 ctgctgcccg cgtcacctgc ggatatcaat attatctggc cgatgatctt atgtggcgct
     1741 ggatttggct tgttccagtc acccaataac cacaccatta ttacctccgc tcctcgcgaa
     1801 cgtagcggtg gagccagtgg catgttagga acggctcgtc tactgggtca gagtagcggc
     1861 gcggcactgg tggcgctgat gctaaatcag ttcggtgata atggtacgca cgtttcgctg
     1921 atggctgcgg ctattctgac agtgattgct gcctgtgtca gtggtttacg tatcactcag
     1981 ccacgatcca gggcataaaa aaagcgcgtc gatcaggacg cgctttttag tatttacttc
     2041 atattacttc aggtattcac ccgtacgcag agcttcaatt cgtttatcca gcggcgggtg
     2101 agtcatgaac aactcactga gcgatttcga cttaccgtta atgcagaaag ccatcatgct
     2161 ggttgcttct tgcggttcat agctggtttt caggcgctgc agcgcggcaa tcattttctc
     2221 gcgaccaacc agttttgccg aaccggcatc agcatggaat tcacgatgac gcgagaacca
     2281 catggtgata atgctcgcca gaataccaaa caccagttcc agaaccgttg caaccgcaaa
     2341 gtagatcagc gggttgccgt tgctctcttc accttcatca cgatttccgc ccataaaacc
     2401 cgcggcaagc tgcgccagaa tacgggaaat aaagataacg aaggtgttca ccacgccctg
     2461 aatcagcgtc atggtgacca tatcaccatt ggcgatgtgg ctgatttcgt gagcaattac
     2521 cgcctcggct tcatccgggc tcatgttctg cagcaaaccg gtgctgacag caaccagaga
     2581 ggcatcacgg cgcgcaccgg ttgcaaaagc gttgatgtcc ggcgcatggt agatagccac
     2641 ttgcggcata gcgatccctg cctgacgagc ctgggttgct acagtattaa ccagccaacg
     2701 ttccctttcg ttacgcggtt gctcgatcac ttccccgcca acagatcgta atgccatcca
     2761 tttggacatc agaagcgaaa cgaaggaacc accaaaaccg aacagcaagg ccatgatcat
     2821 cagcccctga acgctgctcg actgtatccc tgtcaggctc agtaccagcc cgaaaacgac
     2881 cattacggcc aggttcgtta gcaggaagag cgcgattcgc atcataattt tctttttacc
     2941 tcagtttaac aaaacgcaat atgcgatacc cacatcgtat gggttacgcg actattttca
     3001 agtctggata gtgcgtaagt caccagaaag acacaacttt acattttgta gcatctgatt
     3061 tacggcatct tgccgctgtt aaaaaatcag gcacaatttc ttgtgcctga ttgatattac
     3121 ttgacgggag cgggttgttc cgcgggtctg gctttttcaa gcttcgccag atcgagtgcg
     3181 atattcaccg tctcatccag ataaggatcc ggctcctggt aatctttcgg tagatcatcc
     3241 agcttcttca actccggttt accttcgcgt ttaaagcgtt cgttcaaacg cgccagacgc
     3301 gtcgcatcat cttcattatt ctctttctca cgcacagcgt agttcagaga aacgatattg
     3361 cgcttgtcct tcatagcgtt gaagcgcgcg atatccttca tgatgttctg gaactcagga
     3421 tctttcgcga tacgcgcatt atgttccttc agcagctccg gcccaaaggc cgttaaatcg
     3481 cctgatttca cataagtcgc ggcatcaatg ctatcccacg gcagcgcgtt atcttcgaat
     3541 ttctcacccg tttccgtttc ttcattaccc gtcggcatga tgatgtctgg cgttacgcct
     3601 ttacgttgcg tactgccgcc gttaacgcga tagaatttct ggatcgtgta ctgcacagaa
     3661 cccagcgctg gccattcagg acgtaacatc tgatcgtaaa tacggttcaa tgaacggtat
     3721 tgctgaacgg tgcctttacc aaacgtcggt tcacccacaa ccagcgcacg accgtaatcc
     3781 tgcattgccg cggcaaagat ttctgaagcc gaagcactga aacggtcaac cagtaccacc
     3841 agcgggcctt tatagaacac ctgcccgtcg gtatcgctgt cttcacgaac cttgccgttg
     3901 ttatcgcgga cctgaacaat gggacccgca ggaataaaca gacctgagag cgataccgct
     3961 tcggtcaacg ccccaccgcc attgctacgc aggtcgatga tcacactgct gacattctgt
     4021 ttttccagtt tctgcagttg aactttgaca tcgtctgtca aacccacata gaagcccgga
     4081 atatccagca cgccgacttt ctctttaccg acggtcttca ccgacatttt aaccgcgcgg
     4141 tcttcgagac gaatacgttc acgggtcaat gttacagtgc gggtcttggt ccctttacca
     4201 gcaggtaaaa tttccagacg aactttactg cccttcggcc ctttaattaa ggcaaccaca
     4261 tcatcaagac gccagccaat cacgtcaacc atcggcttgc ctgtttgacc aacaccgaca
     4321 attttgtcac caacgctgat agctttactc ttcgctgccg gaccacctgc caccatcgaa
     4381 ttgataacgg tgtagtcatc atccatttgc agcactgcgc caataccttc cagcgacaaa
     4441 ctcatttcag tgttgaactg ttcggtatta cgcggggaaa gatagttggt atgcgggtcg
     4501 atttcacgcg caaacgccgt cattgccagc gagaaaacat cttcgctgtt ggtttgcgcc
     4561 agacgacgaa tggcaaattt gtagcggcgt gtcagggttt cacgaatttc tttatccgtt
     4621 tttcctgcca gcttcaggct taactcgtcg aatttgactt tactgtccca cagcgcgttc
     4681 aactcagcct cgtttttcgg ccagggcgct ttgctgcggt caaggttata agtgtcgttg
     4741 ccggtgaaat ccatcggctt ttccagtacc gacaaagcgt actggtaacg ctcaaaacgg
     4801 cgcttttgcg ccagattgta gagatcgtag aaaacgtcga gtttgcctga acgcagttca
     4861 tcgcctaact cggttttctt tttcgcgaac tgttcaacat cgcttgccag cagcacgttg
     4921 tggctgtaat cgagcagatt caggtagcgg tcaaagattt tggccgaaaa tgcctgatcg
     4981 aggtcgaact ggcgataatg agaacgggtg aagcgcgacg ttacgcgctc acttaccgtc
     5041 gcatgctgcg tctcttcctt taataccgga atttgatcag cacgcgtgat atcttctaca
     5101 gcgaaggtct ggcctgctat tgcaagcagg ccagctaacg cggtaagcct aaaaaacatg
     5161 ttcatgcctg gcccggcctc cgtttcagaa caccaggtgt tctgcgcgca caatcaaaga
     5221 catacccgaa ttcagctgga cgcggacgcc gtctttggtg atttctaata cggtggcatc
     5281 catcgcgttt tgacccgctt tcaccttcag ggcttgtccg acagtcagag ctgaaatgtc
     5341 agaaaccggg gtgtgctgtt cttcgcgagg tgcttttact gtttttggcg ctttctctac
     5401 cggcttttgc gcacgaggtt tacgttcagc gccttctttg cggcgtggcg tggtcggacg
     5461 tggcttgcgt tcgcggcgcg gtgcgtcttc tttctcacca gcagctgcgg cagcttcgcg
     5521 ttttttcgct tgctgttcag cacgctgtgc ctgaacacgc gctttcgctt cttcaagctg
     5581 cttgcgagca tgctctacat gttgctcgtc cagctcaccg catgggttgc cgtcaagatc
     5641 gacacgcgtt gcgccgggtt taacaccgta aagataacgc cagctcgaag tgtagagacg
     5701 taaagcggat cgtaattgcg ttttgctcag gttcatttcc ccggcaacac gatcgaccaa
     5761 atcctgaaaa ataccgattt tcagcggacg cgcttcacct tccgcactga aacagtgggg
     5821 aaaacgttcg gccagaaacg cgattacttc tttactgcta ttcaacttag gttgattttc
     5881 catgaaattt cctgattaca acggacgtag ccaacaagcg caggcatgaa caggcgtcat
     5941 tataatgacg ctaccagtaa atgctacgtt atccgttgat tatcctgcga cgctcgcaaa
     6001 gaattttttg taatccgtcg ttgcaagcac tttttcaagc tgtgccacaa gctgacgtaa
     6061 gccttgctcg tcctcgtctg taaagcgacc gaagacggta ctatcgatgt caagaacacc
     6121 aataatctga tttttcacca ccagcggcag aacaatttca gaattactcg ccgcatcaca
     6181 ggcaatatgc ccgtcaaacg cgtgaacatc ctcgatacgc tgcacttgat tgcgggcaac
     6241 cgcagtgccg cacaccccgc gcccgacggg tatccggaca caggcaattt tgccctgaaa
     6301 tggtccgagt accagtgtat cgtcctcaag caaataaaaa cctgcccagt ttacgtcagt
     6361 gagacgctcg tataacaacg cactggtgtt cgcaagcgtt gccagaaaac tggtttctcc
     6421 cgccatcagc gcgttaaagt cgcgatttaa atccgcgtaa aattctgttt tgttcattat
     6481 ataatcactt gtttgtctta cctggaactg ccagcctatt aaaataagca ttaaatgcgt
     6541 taatgctcaa gatcattccc atcatgggtt aagattaatg ttaattctta ttacatttgg
     6601 cacgtcatgg
//
LOCUS       DAFATA010000167         6386 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFATA010000167 DAFATA010000000
VERSION     DAFATA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.169"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..998)
                     /gene="pcnB"
                     /locus_tag="LGJ20_004512"
     CDS             complement(<1..998)
                     /gene="pcnB"
                     /locus_tag="LGJ20_004512"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBK2388497.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPL"
     gene            complement(1037..1963)
                     /gene="gluQRS"
                     /locus_tag="LGJ20_004513"
     CDS             complement(1037..1963)
                     /gene="gluQRS"
                     /locus_tag="LGJ20_004513"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBK2388498.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2000..2455)
                     /gene="dksA"
                     /locus_tag="LGJ20_004514"
     CDS             complement(2000..2455)
                     /gene="dksA"
                     /locus_tag="LGJ20_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBK2388499.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(2633..3337)
                     /gene="sfsA"
                     /locus_tag="LGJ20_004515"
     CDS             complement(2633..3337)
                     /gene="sfsA"
                     /locus_tag="LGJ20_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK2388500.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(3352..3882)
                     /gene="thpR"
                     /locus_tag="LGJ20_004516"
     CDS             complement(3352..3882)
                     /gene="thpR"
                     /locus_tag="LGJ20_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBK2388501.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            3956..6385
                     /gene="hrpB"
                     /locus_tag="LGJ20_004517"
     CDS             3956..6385
                     /gene="hrpB"
                     /locus_tag="LGJ20_004517"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBK2388502.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1463 a         1650 c         1804 g         1469 t
ORIGIN      
        1 agtgggtacc agaacatggc ggcaaacagg aacgccgggt tcacgcgcat atcgttatgg
       61 atacgcgtat cggtattctt cagcacctgt tcaatgatcc gctccatcgg gctgtcgcca
      121 ttttccgtga agtagcgggt aatggtcggg aacagcggct ggaacagatg atattcacac
      181 aacagcttat aggtatcgta accgtagccc gcttgtagca gtttaagcga ttcttcaaac
      241 aggcgtgccg gtgggatatc gttcaacagg gtagcgaggc gagggatcgg ttctgcggtt
      301 tccgggctga tgcgcatacc caatttggcg gcaaaacgta ccgcgcgcag catacgtacc
      361 ggatcttcac ggtagcgcgt ttccgggtta ccaatcagac ggataacgcc gtccttcaga
      421 tccttcatac cgccaacgta atcacggacg gtaaaatccg ctacgctgta atagaggctg
      481 ttgatagtga aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat gttgtcgcgc
      541 agcaacatgc cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt accttcgtgg
      601 tgtccacgga aggtcgcaac ttcgataatc tccgggccaa acatcacatg agccagacgg
      661 aaacggcgac ccaccaggcg gcagttacgg aacagtttgc gcacctgctc aggcgtggcg
      721 ttagtggtta cgtcaaaatc tttcggcttt ttgccgagta acaggtcgcg cacgccgccg
      781 ccaaccagcc aggcttcgta tcccgcttta ttgagcctgt acattacctt cagggcattt
      841 tcactgatat ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt cacctgtgga
      901 cgggcgactg cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg gcaaaaatta
      961 gcgactcggg taaaaatagt acacctcggt agtgtcaaac atcattcagg acaaaaaaat
     1021 agcggctaat catagctcag catgacgcat ttgagaatgt tgaatttaca attgccgact
     1081 cgggcacggc ggtaagccgc cagtttttga cggctgactg aaggatttgc tcgacgctga
     1141 aatcctgcca gtgtgcttct gcctgctgcc ccagaaattg aagtgccgcg attagtaccg
     1201 ggcgtggatc gcctttcggc aacgcaggcg catgattctg cttggaaagt ttagcgcctt
     1261 gtggattaag cgccagcggc agatgaatgt aatctggcac tttccagcca aaaagctggt
     1321 acagcgagat ttgccttact gttggttcaa tcagatcagc cccacgcact atttctgtaa
     1381 cgccctggaa atgatcatca accacaacag ccaggttgta ggcgaacaac ccatcacggc
     1441 gatgaatgat aaaatcttcc cgtgccagtt tttcgtcggc gtgaataatg ccgcgcagct
     1501 ggtcagtaaa ttgcgtgacc ggatgctgct ggcggatacg cactgcggca ttgtctggtc
     1561 catgatgcaa cacccggcaa tgaccgtcgt aaataccgcc aatgctttga atacgcgcac
     1621 gcgtacaggt gcagtaataa cttagtcctt gttcatgtaa ccaggcgagt gcttcacgat
     1681 aggcgtggtg acgttgcgat tgccagagaa catcgccgtc ccagtgcaga ccgtaatgtt
     1741 ccagctggcg caggatagtt tctgcggcac cgggaacttc acgaggcggg tcgatatctt
     1801 ctatgcgtac cagccagcga ccttgccggg cgcgagcctg caaatagctg ccgagcgcgg
     1861 cgatcagaga gccaaaatga agctcgccgg aaggagaggg ggcgaagcgg ccaatatact
     1921 gtgtgtctgt catctctttg aacaaaaaat aaggcgggag catttcccgc ctgtggtaaa
     1981 cgtgatggaa cggctgtaat tagccagcca tctgtttttc gcgaatttca gccagcgttt
     2041 tgcagtcgat gcacagatcg gctgtcgggc gcgcttccag acggcgaata ccaatttcaa
     2101 caccgcagga ttcgcagtag ccgaaatctt cgtcttccac ttttttcagc gtcttctcga
     2161 tctttttgat cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg aactcttctt
     2221 cctgggctgc acggtctacc gggtccggga agttggctgc ttcatcctgc atatgtgtaa
     2281 cggtgcgatc gacttcatcc ctgagttgat tacgccatgc ttccagaata cgacggaagt
     2341 gcgccagctg ggcttcattc atatactctt cgcccggctt ctcctgatat ggttccaccc
     2401 cagcgatggc gagaatactc agggacgatg ttttacggtt ttgcccttct tgcatgttgc
     2461 ttctccttaa cacgcactat cgatccccat gttcggggga aaaatgaggc cgctataaat
     2521 agcagatgct tttccggata gcaattatct aaacgtaaca cttgacaact gtgtgaggaa
     2581 aagcgtattt gcgcacgcga ccagaatgta aattaaccag ttacttactt tactacaatg
     2641 taaccggcag tgatttttta agagccatgc cttcagcaga aatttccgct ttgtaagcca
     2701 gaatttctac ccccctctgt tgagcttctg acaatagttg cgcgtatttc tcatcgatgt
     2761 ggcgcgcggg tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaag ataaccgcac
     2821 gctggccttc agccgctacg ctcatcaact cccgaaggtg tttctgacct cgttcagtga
     2881 ccgcatcggg aaaatatccc tgttcgttct ccgctaacgt aaccgatttc acttcaatat
     2941 agcagtctgg acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt tctgcgccgt
     3001 attttacttc gcttttcagc gagctatagc ctgacagttc tgaaattgat tcattaagga
     3061 tagcctcttt cgtcaacctg ttagcccaaa gcgtgttgac gcaaataaat gcgccgctct
     3121 ggctttgagt taattcccag gtgtgtgggt atttccgttt ggtgttgtct gaagtcgaat
     3181 accagacggt atcgccaggc gttgcacaac cggtcattgc acccgtattc gggcagtgta
     3241 gcgttaattc gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg taacgctgaa
     3301 ttagcgtcgc gcgctgtaga gggggagaga attccattgc gacaatcctt gttattgcgt
     3361 tagcgcccag cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg aggaggcgta
     3421 aagggtgaac tccgtcaccg cataagacca gttaaaacct ggcggcggga ttgtcaccgc
     3481 ctcgctggcg tcgcgcaata aggtaatatg tggatgaaac ggacgattgc tttgaaaaca
     3541 accgctgcgg gcagcctgtg aacggagcat attcgccagc tggattaagc cgcgtggagg
     3601 ctgacgcatc cctaaccaca ccacacgcga acgcagccat tgtccggcgt catcaagcgt
     3661 gagtgtgaaa ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct tctctttctc
     3721 ggcgctcact tcgcctaaaa atgccagagt cagatgcaaa ttatcggcgg cgaccggacg
     3781 tcccgcctca ggtgggaagt gtgtggcgcg ccaatggata atctgttcgc ggatttctgc
     3841 aggtaagtcg atagcaaaga acagacgttg cggttcagac atgtgaggca ctcggttatg
     3901 aattaccgcg atgctacaat gtggcgcgaa gaatgttaac cctctggagc gttttgtgtc
     3961 gtcgttgccc gttgctgccg tcttatctga attacttacc gctctcgatt gtgcgccgca
     4021 ggtattatta agtgcgccga ccggggccgg gaaatcaacc tggctgccgc tgcaactgct
     4081 ggcgcatccc ggcattaacg ggaaaattat cctgctggag ccgcgtcgtc tggcggcgcg
     4141 taacgtcgcg caacgactgg cggagctgct taacgaaaag ccaggcgata ccgttggcta
     4201 ccggatgcgt gcgcaaaact gcgtcgggcc gaatacccgc ctggaagtgg ttaccgaagg
     4261 cgtgctgacg cgcatgatcc agcgtgaccc ggaactgagc ggtgttggat tggtgatcct
     4321 cgatgaattt catgagcgca gcttgcaggc ggatttggcg ttggcgctgt tactcgatgt
     4381 gcaacaaggt ctgcgtgatg accttaaact gctgattatg tcggctacgc tggataacga
     4441 ccgcttgcag caaatgctgc cagaagcgcc cgtcgtcatc tcagaagggc gctcgtttcc
     4501 ggttgaacgc cattatttac cgctgcccgc gcatcagcgt tttgacgaag ccgttgcggt
     4561 agccaccgcc gaaatgctgc gtcaggaaag cggatcatta ctgttatttt tacctggcgt
     4621 cggagaaatt cagcgtgtgc aggaacaact ggcttcgcgc atcggcagtg atgtgttgct
     4681 ctgcccgctg tatggcgcgt tgtcgctgaa cgatcagcga aaagcgatcc tcccggcacc
     4741 gcaagggatg cgcaaagtgg tgctggcgac caatattgct gaaaccagtt taaccattga
     4801 aggtattcgt ctggtggtgg attgtgccca ggagcgtgtg gcgcgttttg atccgcgcac
     4861 ggggcttacg cgactgatta ctcaacgcgt tagccaggca tccatgacgc agcgtgccgg
     4921 gcgcgccggg cgtctggagc cgggtatcag cctgcattta atcgccaaag aacaagcaga
     4981 acgcgccgcg gcgcaaagtg aaccggagat cttacaaagc gatctttccg gtttgctgat
     5041 ggaattactg caatggggat gcagcgatcc ggcgcagatg agctggctgg atcaaccgcc
     5101 aacggtgaat ctactggccg cgaaacgcct gttacaaatg ctgggggcgc tggagggtga
     5161 acggcttagt gcgcaggggc aaaaaatggc agcgctgggt aacgatccgc gtttagcggc
     5221 aatgctggtt agcgcgaaga acgacgacga agctgctacc gcggcaaaaa ttgccgccat
     5281 tctcgaagag ccgccacgga tgggcaatag tgacctgggc gtggcgtttt cgcgcaatca
     5341 accagcctgg cagcaacgta gtcagcaact gttaaaacgc ttaaacgtac gtggcggtga
     5401 ggcagacagt tcgcttatcg cgccgctact tgccggggcg tttgccgatc gcattgctcg
     5461 tcgccgtggg caagatggac gctatcaact ggcaaacggc atgggagcga tgctcgatgc
     5521 caacgacgcg ctaagccgcc acgaatggtt gatcgcaccg ttattattgc agggcagcgc
     5581 ctcgccggat gcgcggattt tactggcgtt accggtcgat attgatgaat tagtacaacg
     5641 ctgtccgcag ctggtgcagc aatctgacac cgtggaatgg gatgacgcgc aaggtacgct
     5701 gaaagcctgg cgtcggctgc aaatcggtca gttgacggtg aaagtgcagc cactggcgaa
     5761 accgtcagaa gacgagttgc atcaggcgat gcttaacggc attcgtgaaa aaggtttaag
     5821 cgtgctcaac tggacggcgg aagcggaaca gctacgcttg cgtttgttat gcgccgcaaa
     5881 gtggttgccg gaatatgact ggccagcggt tgatgatgaa agtttattgg cgacgctgga
     5941 aacgtggctg ctgccacata tgactggcgt acattcacta cgcggcctga aatcactcga
     6001 catttatcag gcactacgcg gattacttga ttggggaatg cagcaacgtc tggatagtga
     6061 attgcctgcg cattacactg tgccgacggg aagccggatc gccattcgtt atcatgaaga
     6121 taacccgccc gcgctggcgg tgagaatgca agagatgttt ggcgaggcca ccaatccgac
     6181 gatcgcccag gggcgcgtgc cgctggtgct ggagttgctt tcacctgccc aaaggccatt
     6241 acaaatcaca cgagatttga gcgccttctg gaaaggagcg taccgtgagg tgcaaaaaga
     6301 gatgaaaggg cgttatccca aacatgtctg gccggacgac ccggcaaata ctgcaccgac
     6361 gcgacggacg aaaaagtatt cgtaag
//
LOCUS       DAFATA010000168         6353 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFATA010000168 DAFATA010000000
VERSION     DAFATA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.170"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(137..994)
                     /gene="murI"
                     /locus_tag="LGJ20_004518"
     CDS             complement(137..994)
                     /gene="murI"
                     /locus_tag="LGJ20_004518"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK2388503.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(939..2783)
                     /gene="btuB"
                     /locus_tag="LGJ20_004519"
     CDS             complement(939..2783)
                     /gene="btuB"
                     /locus_tag="LGJ20_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBK2388504.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2848..3038)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3152..4252
                     /gene="trmA"
                     /locus_tag="LGJ20_004520"
     CDS             3152..4252
                     /gene="trmA"
                     /locus_tag="LGJ20_004520"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBK2388505.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4292..4651)
                     /locus_tag="LGJ20_004521"
     CDS             complement(4292..4651)
                     /locus_tag="LGJ20_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBK2388506.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4651..5301)
                     /gene="fabR"
                     /locus_tag="LGJ20_004522"
     CDS             complement(4651..5301)
                     /gene="fabR"
                     /locus_tag="LGJ20_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBK2388507.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5632..>6353
                     /locus_tag="LGJ20_004523"
     CDS             5632..>6353
                     /locus_tag="LGJ20_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK2388508.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEY"
BASE COUNT         1661 a         1653 c         1537 g         1502 t
ORIGIN      
        1 ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg
       61 cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt ttctgaccgc
      121 gcaacattca accaaatcag cctaaaactg ccagtttttc gagcgtttcg aagccgtaac
      181 gctgtaaaac gggcaataat tgttcagctt ctggcgtcat tgccatacaa aaggcaatat
      241 tcgcatcggc agattttgca tccggagctt catgttctaa cagccaggcc gttctgcgag
      301 caatcgctgc gccagaatcc accagccggg ttccctctgg cagcacctgt aagagttctt
      361 cttgtagtag agggaaatgg gtgcaaccca atacaacggt atctggcggc tctttcattc
      421 ttaaccacgg gcgtaggata cgttttagtg catccagaga aacatcttcg ccatgcagct
      481 tcgcttcagc caactcaacc atctctgccg agcccagcat ttctatctgg cattcattag
      541 cgaaacgcgc gatcagctca tgagtataag aacgtttaac agttccgcgg gttgccagta
      601 atccgacaat gccatttgcc gtcagacgtg cagcaggttt aatcgccggc acgacaccaa
      661 caaccgggaa gtcgaacttt tcgcgtaatg caggaagtga aacggtactg gcggtgttgc
      721 aagcgaccac agccagcgca aggggataac gttcttgcac tgcggtgaca attgccacca
      781 ctcgctcaac aataaactct tcgctttttt cgccatacgg aaaagcgacg ttatcgaaag
      841 cataaatgta atggagatcc ggtaagagat gccggatctc gtcatagacc gacaacccac
      901 cgacgccgga gtcaaacacc agcacggtgg gacgtggttc agaaggtgta gctgccagac
      961 aaggtgtatt cccgtcctgc agtttggtag ccatagactg tctcataatc tttgtcgaac
     1021 aggttggcta ttttaccacg aactgtcagg tgagaggtga ccggatacgc aaccgcaaga
     1081 tcccacaagc tcacaccgcc cattttaacg gtttgataag gataagatga gtaatcctta
     1141 tcatagcgag tgcctaaata ctgataagta ataccccagt cgaagtcata caactgccag
     1201 tcgagctggt atttcacctg ctgtttagca cggcgtaaca acggcgtgtc ggtaatcgca
     1261 ttgcgcgcat cgacataatc ataactcaca gtatgcgtca gtggtccggt atcaaaattg
     1321 gcggtcgcct cgacgccctt aatccgcgct ttcccttcgt tgtaatattt cagggtgtga
     1381 tcatcataat cgatcaagtc actgacatcg ttacgatatc cggaaatacg ccagttcacc
     1441 ccagcggtta agccttcaaa cgcgccttcc cactgtttgc ttttctccgg gtccagattc
     1501 ggatttccgt agaagccata cagttgcccc agatttggtg ccttataaga tgtcccgtag
     1561 gaagcaatga agcgataacc ttcgatgaat tcccaaccgg cgctggtttg ccaggttcca
     1621 tgacgaccaa actgtgagtt atcgtcactg cgtgctgcgc cttcaaaggt aaaatcgccg
     1681 acttgttgca gcccggtcag atagatgccg gtattacgtt gatcatatcc attctcaaca
     1741 taacctgtac ccggcgtcgt agtctgtttc tgccagtcga cacccgcacc aatactaccg
     1801 tgaccaacga tgacattgtt tgcccactgg acggtgtatt gcttcatctc atcgagcgtc
     1861 gccgacgaat cataacgacc aaaatgggga tcgtagttgt aatctttgct atggctatag
     1921 ctggtaatga gttgtgattt aatcagttcg ccgttatagc gcagcccggc gtcccaactt
     1981 tggctataga gtttacgggt atcgagcaac ggtgaaccgg gagaataata cgcgtcataa
     2041 ttggtacggt tatcatagcc atagccgcgc acaaagccgc tccaggcatc agtaaagtta
     2101 tgctccagcg cgccataaag cgttttactt aaaaaaccat cgttatctgt ctgcgcttgc
     2161 gttccggtat taccataggc aacaacatca taaccatgag tatgggcata atcgcccaac
     2221 agcgttaccc gtgtcttatc ccccagttgt tgctgcgtag agacatcata gttctgataa
     2281 ctattgcttc cccaccctgc tgaaatttcc gttccgggtt catcgcgcgt cgtgatgata
     2341 ttcaccaccc cgcctattgc atcggaacca taaacagcgg agcgcggccc acggatatat
     2401 tcaacacgct ggacaagcgc aatagggaac tggctaaggt cggcagaacc actcaccccc
     2461 gccagattca ggcgtacgcc atcaattaac accaacacat gactggcatt tgtaccgcga
     2521 ataaaaatag atgagagctg acctgaaccg ccgttttggg tgatatcgac gcccggaaga
     2581 cggcgcagca catcattgac cgaggtcgac tgccagcggt cgatatcctg acgggtcaca
     2641 acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa aacggttagc agtaacgacg
     2701 agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg ctgtgacaga acacgccgtc
     2761 agcagcgaag cttttttaat cattgtaaag catccacaat agaagaagga tgccgcaggt
     2821 ttcatcaata ttacgcgatg atgagaacca gatgcgacgt tggccggcag gtcttcgggc
     2881 ttggaggggt atctaagata ctaagagatg atgacttccc accggatggc agtgtccgca
     2941 taacgcaatc atcgcacctt tccttaccgc tgcgcgtcag ctccagattc gcactggatt
     3001 ccctattaac tcacaggacc ggcaagtgga tgctacaggt tgtaacaagt tactgtccag
     3061 acgtagctca caaataggaa ttcatcaaga tctggacatc tgatgagcaa tccctacaat
     3121 cgccgcgtac tttaattttt caggatacat catgaccccc gaacaccttc caacagaaca
     3181 gtatgaagcg cagttagccg aaaaagtggt acgtttgcaa agtatgatgg caccgttttc
     3241 tgacctggtt ccggaagtgt ttcgctcgcc ggtcagtcat taccggatgc gtgcggagtt
     3301 ccgcatctgg cacgatggcg atgacctgta tcacatcatt ttcgatcaac aaaccaaaag
     3361 ccgcatccgc gtggatagct tccccgctgc cagtgaactc atcaaccagt tgatgacggc
     3421 gatgattgcg ggtgtgcgta ataatcccgt tctgcgccac aagttgttcc agattgatta
     3481 cctcaccacg ctgagtaatc aggcggtggt ttccctgttg taccataaaa agctggatga
     3541 tgagtggcgt caggaagcgg aagccctgcg cgatgcactg cgcgcgcaga atctgaatgt
     3601 gcatctgatt ggtcgggcaa cgaaaaccaa aatcgagctg gatcaagatt acatcgatga
     3661 acgtctgccg gtcgcaggga aagagatgat ctaccgtcag gtagaaaaca gttttaccca
     3721 gccgaacgcg gcgatgaata ttcagatgct ggaatgggcg ctggacgtaa ccaaaggctc
     3781 aaaaggcgat ttactggagc tgtactgcgg caacggtaac ttttcattag cactggcgcg
     3841 caattttgat cgggtattag ccaccgaaat cgctaagccg tcggttgctg ctgcgcaata
     3901 caacatcgca gctaaccata ttgataacgt acaaattatt cgtatggcgg cagaagaatt
     3961 tactcaggcg atgaatggtg tacgcgagtt taatcgcctg caagggatag acttgaagag
     4021 ttatcagtgc gaaaccattt ttgtcgaccc accgcgcagc ggtctggaca gtgaaaccga
     4081 gaaaatggtg caggcgtatc cacgtattct gtatatctcc tgcaatccgg aaacgttatg
     4141 caagaatctg gaaacattaa gccagacgca caaggtcgaa cgtctggctc tgtttgatca
     4201 gttcccctac acgcaccata tggagtgcgg cgtattactg accgcgaagt aaaacccatg
     4261 ccggatgcgc cagcatccgg cataataccg attactcagc aacttcctgc ttacggttac
     4321 gcatcttcgc gccaatccag aacaccataa tgacggagag tactgccggg aagaagttag
     4381 agccgatatc cggatattcc gcacgaacta ccgtgctgta cagcagtacg ccgagaataa
     4441 aacaggcagc tgccagaccc ggcaagccta ccggcatggt gcgattaaga taacgttgat
     4501 gcaggcagta aaccgtcagc accagggaaa taatcgggaa tacagaaaat ggcacaatgg
     4561 agctaaacag cgctgcgaaa gtaccattaa tcgataagcc agcaaccaac gccagcagca
     4621 gcgtacctct atcttgattt gcttgtttca ttactcgtcc ttcacatttc ccggaataat
     4681 tgcggttttc tcttgttcac ggcgatacca gtaataagcc cctttcgaaa tcattcgcag
     4741 ttgcagtacc agtcgctctt ctaattgccg acgttgttcg acgccgacgt ccaacgcctc
     4801 ggcacccgca ctgaagacaa ttgtcaccat tgcttcggct tgcgcttcag taaacgcacg
     4861 cggcatatgg ttttcgagtt ccagatagtc cgcaagttcc gcaatgaagt gctgaatttc
     4921 acgcgcaacg gcggcacgaa acgcagcgga ggtgccggag cgttcccgca ataataaccg
     4981 gaaggcgtta ggattattac cgatgaactc cataaatgtg gagaccgagg tgcggatcac
     5041 actcccgcct ttggcgatac gctgacgcgc ctggcgcatg agttggcgta gcattaaacc
     5101 gctctcatca accatggtca gacccagttc gtctacgtcg cggaaatgcc gataaaaaga
     5161 ggtgggagca atgcccgctt cacgcgccac ttcacgcaaa ctcaggctgg cgaagctgcg
     5221 ttcagcactt aattggctaa atgcggcttc caccagcgaa cggcgggttt tttctttttg
     5281 ttgcgctctt acgcccatca cgatgtctga atccttgcca aaagtactgc tggcactata
     5341 ccagagaatg aacatactgg attgctccag tattgtgaat gaacggtaac gcaataaaac
     5401 atatttactg caattctttg gccggttctt ttacgtacag cggaaacctg ccgcttaaac
     5461 ggagagtatc gtcgataaaa atccaataaa acgtcagggc aaaagtaaga aacagacaaa
     5521 gcaaaggccg ctcaggatat agccagataa atgacgggga tcaattggct tacccgcgat
     5581 aaaatgttac cattctgttg cttttatgta taagaacagg taagccctac catgccacat
     5641 tcctacgatt acgatgccat agtaataggt tccggccccg gcggcgaagg cgctgcaatg
     5701 ggcctggtta agcaaggtgc gcgcgtcgca gttatcgagc gttatcaaaa tgttggcggc
     5761 ggttgcaccc actggggcac catcccgtcg aaagctctcc gtcacgccgt cagccgcatt
     5821 atagaattca atcaaaaccc actttacagc gaccattccc gactgctccg ctcttctttt
     5881 gccgatatcc ttaaccatgc cgataacgtg attaatcaac aaacgcgcat gcgtcaggga
     5941 ttttacgaac gtaatcactg tgaaatattg cagggaaacg ctcgctttgt tgacgagcat
     6001 acgttggcgc tggattgccc ggacggcagc gttgaaacac taaccgctga aaaatttgtt
     6061 attgcctgcg gctctcgtcc atatcatcca acagatgttg atttcaccca tccacgcatt
     6121 tacgacagcg actcaattct tagcatgcac cacgaaccgc gccatgtact tatctatggt
     6181 gctggagtga tcggctgtga atatgcgtcg atcttccgcg gtatggatgt aaaagtggat
     6241 ctgatcaaca cccgcgatcg cctgctggca tttctcgatc aagagatgtc agattctctc
     6301 tcctatcact tctggaacag tggcgtagtg attcgtcaca acgaagagta cga
//
LOCUS       DAFATA010000169         6345 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFATA010000169 DAFATA010000000
VERSION     DAFATA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.171"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..128
                     /locus_tag="LGJ20_004524"
     CDS             <1..128
                     /locus_tag="LGJ20_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBK2388509.1"
                     /translation="AALLLDERLTGLQFLGAVLIMTGLYINVFGLRWRKAVKVRG"
     gene            complement(173..871)
                     /locus_tag="LGJ20_004525"
     CDS             complement(173..871)
                     /locus_tag="LGJ20_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2388510.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            1070..1381
                     /locus_tag="LGJ20_004526"
     CDS             1070..1381
                     /locus_tag="LGJ20_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK2388511.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            1496..2818
                     /locus_tag="LGJ20_004527"
     CDS             1496..2818
                     /locus_tag="LGJ20_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK2388512.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            2846..3157
                     /locus_tag="LGJ20_004528"
     CDS             2846..3157
                     /locus_tag="LGJ20_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK2388513.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            3213..4886
                     /locus_tag="LGJ20_004529"
     CDS             3213..4886
                     /locus_tag="LGJ20_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBK2388514.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHDRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            4911..>6345
                     /locus_tag="LGJ20_004530"
     CDS             4911..>6345
                     /locus_tag="LGJ20_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK2388515.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYAQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGEN"
BASE COUNT         1801 a         1253 c         1455 g         1836 t
ORIGIN      
        1 gtgcggcact attgttggat gaacgcttaa cgggtctgca atttttaggt gcggtgctca
       61 ttatgaccgg gctgtatatc aatgtatttg gcttgcggtg gcgtaaagcg gtaaaggtta
      121 gaggctaaac catacgcaca gaatgtgatg tcagcactct gtgcgtctgc ttttatcgcg
      181 ggaaaaagta ctttacctgg aactcgaaaa tattgctgta aagtaacgaa taatccagcg
      241 gaatagagtt actgtccaca gcctgggtct gaattttaag tatgggatcc cgtgggctga
      301 tccgtaaaat actggcgatt tctggggtag gaaaagtagg ggagaaagtt tcaaaggtgc
      361 caatgatttt gatcccgcat tcattctcaa tatatgaaaa tttggatttt tgcatatgtg
      421 acacggttaa atcaggaaaa cgtgctactg acatccacgt ttcttctaat tgtgctgcat
      481 tatcctcaat gaatcgcagt ctctttatgt aataaacctg ctcaccagtc actaaattca
      541 gctcagtcgc aatagtgggt gaagcatcct gcacttcaaa tttcataatc tgactccctg
      601 cctctttgcc gcttttatgc gcaatttcac tgaaactgtt catgtgatca atgtgtgcaa
      661 caaaattctt tttttgaatg tatgttcctg aaccatgctt tctgtgaata attccttcgc
      721 cttccagcaa ggataatgcc ttacgcaggg tattacgcga aacattataa cgaacagcga
      781 gttccgcttc tgacggcaac aaatcaccaa catgccatga attgccattg atttcggatt
      841 taagtaagcg ggcaatcttt tgaaaaatca cttttgcacc ttttaagagc cattgaaaac
      901 aaggagttga gtcctgattg gtttatcgta tggccgagtc gtttaataat caccctgatg
      961 ttgaacaact ttatcactgt attactgtga tcaatgtacc aggacgtata gctactgcgg
     1021 gatatgatat ttaaacaatg taatctgtta tataccagta agggaaaata tgaaaaaaat
     1081 attgttagtt tgcgctgcgg gcatgtcaac cagtatgctg gttaaacgta tgattgatca
     1141 tgctaacgct atttcacttg aagttaatat ttccgcattg gcgattgcag aggctaaagg
     1201 gaaaattaaa aataacgaag ttgacgttgt tttacttggc ccgcaagttc gctttcagaa
     1261 accagagatt gaagccgttg cacaggggaa aatgcctgta gccgttatcg agatgaaaga
     1321 ctacggcacc atgaacggac aggcagttct tgaatttgcg atgaaactac tgcaagagta
     1381 agaatctaat attattaaac tattacctga actaactttt cacacatcat aaaatggata
     1441 tggcatacat gctttattgg gatgtataaa tatccaaaac aacaggtata aaaaaatggg
     1501 attaatggcc tcattcgaac gtggaatgga acgttttctt gttccagttg ctatcaagtt
     1561 aaactcacaa aaacatgttg cagcggtgag agatggattc gtttttacgt ttccaattat
     1621 catggcaagt tcattaatta tattaattaa ctttgccata ttatcgccag acggttttat
     1681 tgccggatta ctgcatctga atagcatttt ccccaacctt gaaaaagcac aagctatttt
     1741 tactccggta atgaatggtt ctgtaaatat catgtcaatt atgattgctt tcctggtcgc
     1801 caggaatatg gcgattagct atgagcaaga tgatctttta tgcggattaa cggcaatagg
     1861 agcatttttt attgtttata cgccatatca gatgatagat gggcaagcat tcctgacgac
     1921 caaatatctc ggcgcgcagg ggttgtttgt tgctgttatc gttgcattga tcaccagtga
     1981 aatattttgt cgcttagctc gaaacccaaa aatcaccata acgatgccgg cagctgtacc
     2041 gcctgcggta gcgcgttcat ttaaagtttt attgccaata ttttttgtca tggtgttctt
     2101 ttccgcactt aattattgcc tgacactgat atccccggca ggactaaacg atcttattta
     2161 cacattaatc cagacgccgc tcaaacatat gggaacgaat atctttgcgg taattatcct
     2221 gggggctgtg ggtaatttcc tgtgggtgct ggggatccac ggacctaata ccacatcggc
     2281 aattcgagaa accgtttttt ctgaggctaa tctggagaat ctctcctggg ccgctcaaca
     2341 tggcactacc tggggcgcgc catatccgat tacctggact tctattaatg atgcattcgc
     2401 caactgcggc ggttcaggta tgacgttggg gttattgttg gctattttta tcgcttctaa
     2461 gcgtgcggaa taccgtgatc tggcaaaaat gtcatttatc cccggtattt tcaatatcaa
     2521 tgaaccgata atgtttggcc ttcctattgt acttaacccc atcatgatgg tgccgtttat
     2581 tatggttccc attgttaact gtgccattgg ttacttcttt gtttcgatgg aaattattcc
     2641 accggttgct tatgccgtgc cctggactac gcccggacct ttaattgctt tcctcggaac
     2701 cggggggaac tggctggctt tactggttgg ttttttatgt ttaggtgtgg cgacaatgat
     2761 ctatttacct tttgttattg ccgccaacaa agtcaataac atgacaacta acggataatt
     2821 ccatttaccc aggaaggaga ttgtgatgtt tgcagatgaa gaacttgtta tggagttact
     2881 ggtcaacgcg gggcaggcac gttctgacgc gatggaagcc atacgttgtg ccgggcaaaa
     2941 agactggcag ggcgcaactc agctaatggc cagttctgaa tctgcctgtc ttcaggcaca
     3001 taagattcag accgcattaa ttagtcagga tgaaggctgt ggcaaaataa aagttaattt
     3061 aattctgatt catgcacagg atcatttgat gaatgcaata ttatgccagg atttagccag
     3121 ggaaattatt tcattaagaa aagaactcca tgcctgattt tttcctccag tgcgatagct
     3181 ggaggaaatt attatactct taaggataag tcatgaatat aaagacgctt aacgtcagcc
     3241 ttttgtcttt ttctattatt acagcattgt ttccattgaa cgcgatggca acaaaattaa
     3301 ccatagagca gcgccttgaa ttgcttgaaa atgaattgtc gcaaaataaa caagagctga
     3361 aagcaacaca gaatgaactg ggagtatata aatcccgact ttcgacctta caaaaaagca
     3421 tcacggaaaa caaatataaa tcagcctcgc ttgctgaaat atcagcgaca tctcccgtcg
     3481 ctgataacat caaaaatgaa aacggtgaac agaactcgtc tgccgcagcg catactataa
     3541 atggatcgca gcaagttgcc gttattgaaa gtaaaggcga caaaaccact atcgaaagcg
     3601 tgaccctgaa agatatcagt aaatatataa aggatgatat tgggttcagc tatcaggggt
     3661 acttccgctc aggctggggt actggtaatc acggctcacc acaaacttat gcagcgggtt
     3721 ctctgggacg ttttggtaat gagatgagcg gttggtttga cctgacctta aatcagcgtg
     3781 tttataatca ggacggtaaa acggcaaatg cggtcgttac ctatgatggc aacgtgggtg
     3841 agcagtataa cgatgcctgg tttggtgaca gtgccaatga aaatatcatg cagttcagcg
     3901 atatttacct gacaacgcga ggttttttac ccttcgcacc agaggcagac ttctgggtcg
     3961 gcaaacataa actcccgcaa tatgagatcc agatgctgga ctggaaaacc ttaaccacgg
     4021 atgtcgccgc gggtgtgggg attgaaaact gggcacttgg tgtagggctg tttgatatgt
     4081 ccttaagccg tgatgatgtc gatgtttact ctcgtgattt ttcgcgtacc agccagatga
     4141 ataccaattc tgtggatgtt cgttatcgca atatcccgtt atgggatgat gcgacattat
     4201 cattaatggc taaatattcc gcacctaata aaacggatca acaacaagat aacgaaaatg
     4261 acaacagtta ttttgaaatg aaagatagct ggatgctgac ttctgtttta cggcaaaacc
     4321 tgcaacgcga tacgtttaat gaatttacgt tacaggttgc caataattcc tatgccagca
     4381 gttttgccag tttctcagat gccagtaata cgatggcgca tgatcgctat tactatggtg
     4441 atcataccaa tgggatcgcc tggcgtttaa tctctcaggg cgagatgtat cttactgaca
     4501 atattattat ggctaacgcg cttgtctatt ctcacggcga agatgtttat agttatgaaa
     4561 gtggtgctca tagtgatttt gacagtattc gcactgtaat aagaccggcc tggatctgga
     4621 atacatggaa tcagacgggg cttgaattag gctggtttaa gcaacagaac aaaactcagc
     4681 agggtgtaac actaaatgaa tcggcttata aaacgacact ctggcatgca ttgaaagtgg
     4741 gtgaaagcat tttaggttca cgaccagaaa ttcgcttcta tggaacgtat atcaatattc
     4801 tggataacga attatcgaat tttaagttta atgagaacag caaagacgag tttatggccg
     4861 gcatccaggc ggaagtctgg tggtaatcta ttttagttaa ggagttgtgt atgtcaggat
     4921 ttaaaaaagg ttttttatgg ggcggtgcgg tagccgcgca tcagttggaa ggtggctgga
     4981 atgaaggagg aaaaggcatc agcatcgctg atgtgatgac tgctggcgct cacggggtgc
     5041 cgcgtgaagt gacagaaggc gttatcgacg ggcttaatta tcccaatcat gaagcaattg
     5101 atttttatca tcgctataaa acagatattc agttatttgc cgagatggga ttcaaatgct
     5161 ttcgaacttc cattgcctgg acacgaatct ttccgcaagg tgacgaacag gagccgaatg
     5221 aagagggttt acaattttat gatgatctgt tcgatgaatg ccttaagcag ggaatggaac
     5281 ctgtggtgac gctttcacat tttgagatgc cttatcatct ggtgacaaaa tatggtggct
     5341 ggcgaaaccg taaactgatc gactttttta tccgcttcgc atcaacggtc ttcacgcgct
     5401 ataaagaaaa agtaaagtac tggatgacgt ttaacgaaat caataatcag gtgaatttca
     5461 gcgaaagcct gtgtccattt actaattccg gtatcttgta ttcgccagag gaagatatca
     5521 atgagcgcga acaaataatg taccaggcgg tacattacga gttagttgcc agtgccctgg
     5581 cggtacagac tggaaaatcg atcaatcctg aatttaacat tggctgtatg atcgccatgt
     5641 gtcccatcta tcctctgacg tgtgcaccca acgatatgat gatggccacg aaagcaatgc
     5701 atcgtcgtta ctggtttact gatgttcatg ctcgtggata ttatgcgcaa catatgctga
     5761 attactttgc caggaaagga ttcaacctcg atatcacacc agaagataac gcgattcttg
     5821 ccagtggttg tgtcgacttt atcggcttta gctactacat gtcttttacg acacaatttt
     5881 cgcccgataa cccgcaactg gattatgttg aaccacgaga tttggtcagc aacccttata
     5941 tcgatacatc cgaatgggga tggcaaattg atccggcagg gctacgttat tcactcaact
     6001 ggttctggga tcatttccag ttgccgctgt ttattgtcga aaatggattt ggtgcggttg
     6061 accagagaca agctgacggc acggtgaacg atcactatcg cattgattac tttgcttccc
     6121 atattcggga aatgaaaaaa gccgtagttg aagatggtgt tgacttaatt ggctacaccc
     6181 cgtggggctg cattgacctg gtttctgccg gaacagggga aatgaaaaag cgctacggaa
     6241 tgatttatgt cgataaagac aacgaaggga agggaacgct ggaacggata cgtaaagcgt
     6301 cgttttactg gtatcgggat ctcatcgcca acaatggcga aaata
//
LOCUS       DAFATA010000170         6317 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFATA010000170 DAFATA010000000
VERSION     DAFATA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.172"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(137..1459)
                     /gene="xylA"
                     /locus_tag="LGJ20_004531"
     CDS             complement(137..1459)
                     /gene="xylA"
                     /locus_tag="LGJ20_004531"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBK2388516.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            1825..2817
                     /gene="xylF"
                     /locus_tag="LGJ20_004532"
     CDS             1825..2817
                     /gene="xylF"
                     /locus_tag="LGJ20_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2388517.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            2895..4436
                     /gene="xylG"
                     /locus_tag="LGJ20_004533"
     CDS             2895..4436
                     /gene="xylG"
                     /locus_tag="LGJ20_004533"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBK2388518.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            4414..5595
                     /gene="xylH"
                     /locus_tag="LGJ20_004534"
     CDS             4414..5595
                     /gene="xylH"
                     /locus_tag="LGJ20_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBK2388519.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            5673..>6317
                     /gene="xylR"
                     /locus_tag="LGJ20_004535"
     CDS             5673..>6317
                     /gene="xylR"
                     /locus_tag="LGJ20_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HBK2388520.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCV"
BASE COUNT         1654 a         1505 c         1544 g         1614 t
ORIGIN      
        1 tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc tatcccgata
       61 tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc gggccaacgg
      121 actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt tccagttgct
      181 cctggcgacc actctgatgc accggagaca aattatgttc ctgagcatat ttggctaaat
      241 ctgccagtga catttggcct ttcaggattt gctggcccaa ttcgctattc cagccggaat
      301 aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgcgct gcaattttca
      361 gcgccagtgc catcgtatcc atcgcgccga tatgaccgta aaacagatca tatttatcag
      421 tactttgacg acgtactttg gcatcgaagt tcagaccacc ggtggtgaaa ccgcctgctt
      481 tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac tggtcggtgt
      541 cccagcccag ttgcgcatcg ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa
      601 tggcggtggc aatttcatga tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa
      661 tgttcagttt aatctctttt tccagaccaa actgtttcag gaagccatag accgtcgcgg
      721 catcgtaatc atattgatgt ttggtcggtt cttgcggttt cggttcgata agcaacgtgc
      781 cctggaaacc gattttatgt ttatgctcaa ccaccatctg cataaagcgg cccagctgtt
      841 cacgctcctg acgcaagtcg gtatttaaca gcgtttcgta accttcacga ccgccccaca
      901 ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca acttgcgttg
      961 ccgcccaact gaagacttca ggatctgggt tcgtcgccgc acccgcgcca tagcgagggt
     1021 ttgtaaagca gttagcggtt ccccacagca gcttcacgcc gctctcttct tgcttgcctg
     1081 ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac gcgccctcag
     1141 gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg aaaaactcaa
     1201 atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc tgccacggac
     1261 gattaaacgc ccccacacca aacatgtccg ccccgttcca gcagaatgtg tgccagtagc
     1321 aggcggcaaa acgcaagtgc tcttccatac gcttgcccaa caccagttcg tcgggattgt
     1381 agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg cgatcgagct
     1441 ggtcaaaata ggcttgcata ttgaactcca taatcaggta atgccgcggg tgatggatga
     1501 tgtcgtaata ttgggcactc cctttcagtt gctcaattat gttatttcac actgctattg
     1561 agataattca caagtgtgcg ctcgctcgca aaataaaatg gaatgatgaa actgggtaat
     1621 tcctcgaaga gaaaaatgca ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata
     1681 aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt gttttcccct
     1741 gtttagttgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt aaagctgtcc
     1801 tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac cctttgcacc
     1861 tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg tatggcgatt
     1921 gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa aaaggcagaa
     1981 tctctcggcg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac acaaatgtcg
     2041 cagattgaaa acatgataaa ccggggtgtc gatgttcttg tcattattcc gtataacggt
     2101 caggtattaa gtaacgttgt aaaagaagcc aaacaagaag gtattaaagt attagcttac
     2161 gatcgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa cgaaaaagtc
     2221 ggtgaactgc aggcaaaagc cctggtcgat attgttccgc aaggtaatta cttcctgatg
     2281 ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat gaaagtgtta
     2341 aaaccttacg ttgattccgg aaaaattaaa gtcgttggtg accaatgggt tgatggctgg
     2401 ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa taacaaaatt
     2461 gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc attaagcgcg
     2521 caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc aggtattaaa
     2581 cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacgtt gttggcaaat
     2641 actgccgcag aaattgccgt tgagttgggc aatggtcagg aaccaaaagc agataccaca
     2701 ctgaataatg gcctgaaaga tgtgccctcc cgcctcctga caccgatcga tgtgaataaa
     2761 aacaacatca aagacacggt agttaaagac ggattccaca aagagagcga gctgtaagcg
     2821 ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa tgaattggct
     2881 taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa accttcggca
     2941 gtgtgaaggc gattgataac gtctgcttgc ggttgaatgc tggcgaaatc gtctcacttt
     3001 gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc
     3061 atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg agtcacatcc
     3121 gcgataccga acgcaaaggt atcgccatca ttcatcagga attggccctg gtgaaagaat
     3181 tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc attatggatt
     3241 atgacctgat gacgctacgc tgtcagaagc tgctcgcaca ggtcagttta tccatttcac
     3301 ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa attgccaagg
     3361 cacttaataa acaggtgcgc ttgttaattc tcgatgaacc gacagcctca ttaactgagc
     3421 aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt atcgcctgta
     3481 tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt tgcgttattc
     3541 gcgacggaca gcacattggt acgcgtgatg ctgccggaat gagtgaagac gatattatca
     3601 ccatgatggt cgggcgagag ttaaccgcgc tttaccctaa tgaaccacat accaccggag
     3661 atgaaatatt acgtattgaa catctgacgg catggcatcc ggtcaatcgt catattaaac
     3721 gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt gccggactcg
     3781 ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc ggacaatggg
     3841 aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag caagccatcg
     3901 cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg
     3961 cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc attagccagc
     4021 ttgatgacgc ggaagagcaa aaatgtattc tggaatcaat tcagcaactc aaagttaaaa
     4081 cgtcgtcccc cgatcttgct attggacgtt tgagcggcgg caatcagcaa aaagcgatcc
     4141 tcgcccgctg tctgttactc aacccgcgca ttctcattct tgatgaacct accaggggta
     4201 ttgatattgg cgcgaaatat gagatctaca aattaattaa ccaactcgtc cagcagggta
     4261 ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc gatcgtgtac
     4321 tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg actcaggagc
     4381 aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg
     4441 aaattggccg taccgacatc cggtggcttt tctgggctga aatcactgaa tttgcaggtc
     4501 ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac caccgatggt
     4561 gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc agaccgcgat taccggcatc
     4621 ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc cgtcggctca
     4681 atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg ctggcctttg
     4741 ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcctg gaacggatgg
     4801 tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat gttggcattt
     4861 cgcggcatac tcattggcat caccaacggc acgactgtat cccccaccag cgccgcgatg
     4921 tcacaaattg ggcaaagcta tctccccgcc agtaccggct tcatcattgg cgcgcttggc
     4981 ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc tttgggttta
     5041 cagtctccgg cctctaccgc agtagtcggt cgccaggctt taaccgctat catcgtatta
     5101 ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct gctgctgacg
     5161 ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg acgcatttat
     5221 gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga acgcaccaaa
     5281 cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat ccttagttct
     5341 cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc aattgcagca
     5401 tgcgtgattg gcggcaccag cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg
     5461 ggggcattta tcatggcttc actggataac ggcatgagta tgatggatgt accgaccttc
     5521 tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga ctccgcaacc
     5581 aaacgccgtt cttgattttg attaaaattt tctcaaagcc ggttacgtat taccggtttt
     5641 gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac cgcatcacat
     5701 tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta ggggaatatt
     5761 tacaggcgtc acaatcggaa tgggatattt tcattgaaga agatttccgc gcccgcattg
     5821 ataaaatcaa ggactggtta ggagatggcg tcattgccga cttcgacgac aaacagatcg
     5881 agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg tatcaccttg
     5941 cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg gttgaaagcg
     6001 catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt cttccggaat
     6061 caagcggcaa acgttgggcc actgagcgcg aatatgcatt tcgtcagctt gtcgccgaag
     6121 aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac tggcaacacg
     6181 cgcaaaatcg gctggcagac tggctacaaa cgctaccacc gcaaaccggg attattgccg
     6241 ttactgacgc ccgagcgcgg catattctgc aagtatgtga acatctacat attcccgtac
     6301 cggaaaaatt atgcgtg
//
LOCUS       DAFATA010000171         6215 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFATA010000171 DAFATA010000000
VERSION     DAFATA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.173"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..611)
                     /locus_tag="LGJ20_004536"
     CDS             complement(<1..611)
                     /locus_tag="LGJ20_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK2388521.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGG"
     gene            complement(657..1415)
                     /locus_tag="LGJ20_004537"
     CDS             complement(657..1415)
                     /locus_tag="LGJ20_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388522.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(1447..2160)
                     /locus_tag="LGJ20_004538"
     CDS             complement(1447..2160)
                     /locus_tag="LGJ20_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBK2388523.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            2334..3026
                     /locus_tag="LGJ20_004539"
     CDS             2334..3026
                     /locus_tag="LGJ20_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBK2388524.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3075..4574)
                     /gene="pitB"
                     /locus_tag="LGJ20_004540"
     CDS             complement(3075..4574)
                     /gene="pitB"
                     /locus_tag="LGJ20_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBK2388525.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(4866..>6215)
                     /gene="gss"
                     /locus_tag="LGJ20_004541"
     CDS             complement(4866..>6215)
                     /gene="gss"
                     /locus_tag="LGJ20_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBK2388526.1"
                     /translation="NGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGA
                     RLENKGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHL
                     MYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYE
                     YNADSASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDK
                     DIEENYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWET
                     AFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPIL
                     WSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAE
                     QKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
BASE COUNT         1599 a         1618 c         1487 g         1511 t
ORIGIN      
        1 ccgccaacca gattcgacac gtaccaggca ataacaaaac ccgcaaaacc aaagtcaaaa
       61 taccaggcta cgacgctccc cgctgcgcgc agaaaaggtt tcgtcgcctg ctgtacagca
      121 attaaatcga agcgatctac cgcacgcaga atgccggtcg gcgtggagga agccattgaa
      181 ggaatgagcg tgcaatacag cgccgccagc caaaagcttt ggtcatccag acctaatgaa
      241 tgggaaagga atggcagtaa ggcaatgccg ccgacaatcg ccaccgcgcc gctgacgata
      301 tccagcgaga aggaaaatga gacgacattg cgaaattgct gcggattatt gttggtcaag
      361 gctggcgttc cgtactgaac caccagttgc catgtctgaa acttaataaa atcgctgatc
      421 gacttggcgt acgattgcac aatcaccagt acgccaaaca tggcgggcgt catcccttta
      481 ccggcacacg agagcgccaa cagacccagc aaggcgctca cgacattact ggagcctaac
      541 caggcgctat tgcgaataat ggtgcgaaac gcgccatctg caaaccaatg tttgatgtta
      601 aaacccgcca attcagcctg accttatcgt tgatagtaaa aagtatcccg ccagccttaa
      661 gttaaacttc ggcggtcaga aacgatggca accagagaaa ccgccttctg tgcctgttcc
      721 agcacttcgc tgtagggcgc tctggaatca atctcaagaa tttttgtgcc gttatagcca
      781 atcgtcgaca tgacgccgat tttgtcctgc agctcggcat agtcatggtc aggcttgcgg
      841 gagatggcag tctcaatatc aatgcccagg cgaataatta attccgggcg atattgcgcc
      901 atttgttggt ataaacgccg ttcgcgctgc gccagaaaca tgctgatttt cccggtcgca
      961 cgttcgacgc caatccccgg tccatcataa taaaagcccg aaatttcagc ctgcgggaag
     1021 cgatcgctga ccaccagaac gccactttgc gccagtcgct gaaccttgcg tagattcgcc
     1081 attcttcgca gcgagaagca gtacataatc accgccgccc atagcgccgg agatttggtt
     1141 ttcatgcttt gggttttcga ggatttggcc gccagtcgcc gttccagcca gacgccaacc
     1201 aacggcaatc gtttgatttt gtcgccgtct tcgccggaga gcagccccag atagcgccgc
     1261 tcggtttgcc agtgttgttg cagcgatttc accaggtcgg tggtcagtgt ggatttaccg
     1321 gtgccatcac acccgaccac cgcaatcagc cccggaatgt agttgggctg cggcgcggtt
     1381 gtactattaa cagtttgagt ttgtagtgca tccattcgtt aaatcatccc ctatccattt
     1441 tggtgatcag gaaagcgcgg cgtgaattgc gcgtagtgac gcttgcagaa tttcatcggc
     1501 ggggtgtcgc ccatccagtt caaggatctt tgcgccattg aatgtcagtt gcggcgtaac
     1561 ggcgattttt tcctgtaacg ctgccagttg gtggtcaggt ttacgcgcaa acgcggtttg
     1621 ttcatcaatg ccaagacgaa tcaacaatac gggcaaataa gatgccatcc attggtacag
     1681 cttcagctcg cgctgcctta acatttttat ccaaccgtta ccgcccgtgg tttttgccaa
     1741 ttgcgggcca tcaaagcgaa aacccggcac ttcaacctgc gggtagcggt cggtgatgag
     1801 cagaaagcct tgctggcttt tacacaacat tttgcgaaac ttgtacgccc gccagcagga
     1861 aagcagatag ataaccagcg cagtaatatt gccaggcggt gttgagggtt tttcgtgcac
     1921 atgtgccgct ttacttcgca gataacgccc acaaggtgcg ccaataacag ggagctgtga
     1981 aatccattcg ccaattcgcc cggacgattg cccgagataa atgtgttctg ttggcattct
     2041 tgctgccagt tcatttacca ggcttgccgt gagggtcgat ttacctgaac catcacatcc
     2101 tacaatggca ataacacgca ctggcgtgga attaattatt gacatattac gttgattcac
     2161 gaaaaacccg gcagtaaata atgtattgaa tattacgtgg tccgtgctca gaatatccgt
     2221 tcaaccttat tgtgacaatg gttaattgtg acattgacct gaattacgct ttcataaaaa
     2281 catattaacc aaataaatat tttggatgga tatttaaatt taaggatata ttcatgcagt
     2341 caataacacc tccattaatt gccgttattg gtagcgatgg ttcaggcaag tcaacggtgt
     2401 gtgaacatct cattaccgtt gtcgaaaaat atggtgctgc cgaaagagtt catttaggaa
     2461 aacaagccgg aaatgtcggt cgtgcagtga caaaattacc gttgatggga aaatccttac
     2521 ataaaacaat tgaacgaaat caggtgaaaa cagcaaaaaa attgccagga ccagttccgg
     2581 cgctggtaat tacagcgttt gtcgcccgtc gcttactgcg ctttcgtcat atgcttgcct
     2641 gtcgtcgtcg cgggttaatt gttctaaccg accgttatcc tcaggaccaa attcctggcg
     2701 cttacgatgg tacggtgttc ccacctaacg ttgaaggtgg tcgttttgtc tcatggctgg
     2761 caagccagga acgtaaagcg tttcactgga tggcgagcca taagcctgat ctggtcatca
     2821 aactcaatgt cgatctagac gtggcctgcg cgcgtaagcc tgaccacaaa agggaatcgc
     2881 tggcaagaaa gatagccata acgccacagt taacctttgg tggtgcacaa ctggttgata
     2941 tcgatgccaa tcagccactg gaacaggtgt tggttgatgc agaaaaagcg attacggatt
     3001 ttatgaccgc gcgtggttat cactagtcaa aaatggaaat gcccgatcgc caggaccggg
     3061 cattttcagg aaggttaaat caactgcaat gctatccagt acagcccacc agaaagaaaa
     3121 attgccgccg gtaaagtaaa cacccatgcc atcaggatgc tggttaccgt tttacgctgt
     3181 aacccaccgc cgtccaccac catcgtccct gctacggcag atgagagtac gtgtgttgta
     3241 gagacgggca tcccaatata actggcaagg ccgatagaca ctgccgccgt catttgtgca
     3301 gccatgcctt gcgcatacgt catgccgcgc ttaccaatct tctcaccgat ggtcattgct
     3361 acacgacgcc agccaatcat ggtgccaatg ccgagcgcca gtgctaccgc catgatgatc
     3421 cacaccggag cgtactcaat ggtgcttaac atatcgctgc gcagtttttt cagcaggttc
     3481 tggtcttctt tactgacgcc tggcagtttc gccagcttcg cggaggtatc tgagatgcac
     3541 agcataatgc ggcgcagctg gctgcgctga ctcacgctta acggctcgta actttccata
     3601 ttgcctggca gcatcgtttt aacgcgcgca atcgcatcaa aggtatttgc cggatgacag
     3661 tgaaactctg ttacttgcgc gccatcagtc gatgccgcag gcaatggagg ttccatcgca
     3721 atcaacttct gcggcagttc aggatgctgt tgcagatagt gttcgaagtt ggagacggca
     3781 tcgcgagtac gggtaatttc atagccggac gcgttcatat tgacgacgaa gccagcaggg
     3841 gcaatcccca ccagtaccag catcaccagg ccgatccctt tctgcccgtc gttcgcgccg
     3901 tgcgaaaacg ccacgcccgc agcggaaaca atcagcgcaa tacgcgtcca gaatggcggt
     3961 ttacgtttgc cttttttctt tttgcgatct tccggaatgc ggtgaatacg atcacgcttt
     4021 ttcgtcccgc tccagtagcg tcgcagcagg aatatcaggc ctcccgcaat gaccaggccg
     4081 acgatagggg aaacaatcag cgaggagaaa attttggtca cttcacgcag gtttaacgca
     4141 tccatcaccg atgagccggt taacagcgcg ttggttaaac cgatgccgat aatcgcacca
     4201 atcaaggtgt gcgaactgga ggccggtaaa ccgaagaacc acgtccccag gttccatata
     4261 atcgccgcca gcagcatgga aaagaccatc gccagaccgt gggttgaccc catattcagc
     4321 aacaaatcgg ttggtaacat atggacaatg gcataggcaa cgctaagtcc gcccaataac
     4381 acgccaaaaa agttaaaaaa tgccgccatc accacagcaa gttgtggttg catggcacga
     4441 gtataaataa cggttgccac cgcattcgcc gtgtcatgaa aaccattgat tgcttcgtag
     4501 aacaacacaa atgccagagc aagcaataac aaaagccctg tgtatatatc aaggccaaca
     4561 aataaattta gcatatatta gattacgcca ttttgaattt acgaacggac gcattatcag
     4621 tgactttaac ggcatgggca aagtgaaata tcatttttta acgataaaaa gagtgttatt
     4681 tcttgtgtat cagacaataa gcattcattc aaaaccttta gaaataatca aaacacaggt
     4741 attccactgg ttaatatatt cacatatgaa atgaataaat attggaatat ataaatattg
     4801 aatattttga ttaatccccg tactgattat tcttcataat cagtacgggg ttgctacaac
     4861 atgtattact ttttcaccac aattaacggt tcaatatcac tctctttttt gatgaccagt
     4921 gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc caacggtgaa ggtacatacc
     4981 tgaatgtatt taccgtccac tttcggcaaa caccacagtt gctgatagat gtttttctgc
     5041 tcggcaaatt taccgctggt tttgtccagc acctcttcat gatggctgac gaggtcgata
     5101 ttgctgccac agcgaccggc gatcggtttc actgcgtaac ctgttttcac cagttcatca
     5161 ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc
     5221 ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg gctcaaagac cagcacttcc
     5281 gggcgcagca atacgtcgat aagacgcact tcgttttgcg gatgaccggt acggattggc
     5341 accgcagcaa actcacggtc gctaacttca cgaatctgat caaacgcggt ttcccacgcc
     5401 caggttttcc acacgcagtt aaccagccgt ccttccccat caatcagttg cccggcagca
     5461 tcccagccca gttcatccag cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc
     5521 gcctgctcca taaactgcgc gtgatagttt tcctcgatat ctttgtcctg catgatatgg
     5581 acaaacggac gtgcacgact gtgtttccag gcaccagcca gttcgttaat cagcccttcc
     5641 gccggattga agccgttgcc tttatagccc tgctccgccc aacgttcgag gatcaagccc
     5701 gcttcggtat gacatgaggc agagtcggcg ttgtattcgt aaaccttcag gccacgctca
     5761 tccatgcaga aatccatacg accagtgatc atatggtgac ggcgacgctg ccaggagaga
     5821 cgcaaacgtg gccagaggat tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc
     5881 agcaccttgt cggttgcgtg aagatacatc aggtgcagct cgttggtggc tttaattagc
     5941 tcctgctcgg cactctcggt aatggtgtag taatgataag gatcctgatt gatcacctga
     6001 ccgttggcct gcacataggc gttttgcagc ggatcttttt catccagcca tttaccgtca
     6061 aactggcctt tgttttccag gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc
     6121 ggctgcggta agctgtattc agtatcttcc gtctggatca tccagcccag aatggtggtg
     6181 tcatcaaaag tgtctttcag ggtatagcag ccgtt
//
LOCUS       DAFATA010000172         6130 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFATA010000172 DAFATA010000000
VERSION     DAFATA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.174"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(85..846)
                     /gene="envY"
                     /locus_tag="LGJ20_004542"
     CDS             complement(85..846)
                     /gene="envY"
                     /locus_tag="LGJ20_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBK2388527.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1029..1919)
                     /locus_tag="LGJ20_004543"
     CDS             complement(1029..1919)
                     /locus_tag="LGJ20_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBK2388528.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(1920..4892)
                     /gene="nfrA"
                     /locus_tag="LGJ20_004544"
     CDS             complement(1920..4892)
                     /gene="nfrA"
                     /locus_tag="LGJ20_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBK2388529.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWGLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4879..>6130)
                     /gene="nrfB"
                     /locus_tag="LGJ20_004545"
     CDS             complement(4879..>6130)
                     /gene="nrfB"
                     /locus_tag="LGJ20_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBK2388530.1"
                     /translation="WIIGIVFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLV
                     MLQLLLLLAYESLWPNAWHFLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGL
                     TQGLLSVLRLFWGNLINFMANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRP
                     LGQILLENQVITEEQLDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWES
                     IDAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKV
                     RYVIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLF
                     AEILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSM
                     QSLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1385 a         1629 c         1625 g         1491 t
ORIGIN      
        1 agtcgtgggc cgcaggttcg aatcctgcag ggcgcgccat ttcttcttca tttatgcccg
       61 tcttatccgt ttccgctttg cccttcacca catcactttt tgtcgctgtt tggcgagata
      121 attcaacggt gtcaggccgt aaaacgcctt aaaaacagag ataaagtatg acgtgctgct
      181 atagccacat aattgcgcca cctgagtgat atttttgtta tccatcaata acatctgtac
      241 ggcgtaacgc atacgacact ctgtgacaat ctggctatag ctggtatttt cgttttttaa
      301 tttctttttg agcaggctgg ggcttaaaca tagcgaactg gcgacaattc gcagattcca
      361 gtaatgctga atatcgcttt gaataatgcg gcagacgctg tcgcggacgc tgctgcgtaa
      421 gatatacatc agtagggcaa taaaccgcga ttgctcaaga aagttagaca atacggtaaa
      481 aagcaatacg cgcgtcaact ccgcttcgca gggtttgctg ggttgctgct tgctgtggtt
      541 ggcggctagc cggaacactt ccggtgtacg gctatgacaa gcaataaccg gggttgccag
      601 ccgcgaccag ggctttacac aggttaagtt cttatttaaa aacaacagat aatcgttgat
      661 gatgttacgg ttgatgtggg tgattaatac gtgatttaac gttgagagtt caatgacgtt
      721 attgttgcac gcgaaggcgg ccaggtagtt tttcggaagg gtaaacgtag catggttatt
      781 gacgcttacc tctacctctt tttcggtcag gatcaccacg caaggttcac tgctgctcaa
      841 ttgcatttcg cactcctcag atatcagaaa ctccgctcaa aggatctatg cttcctgcat
      901 gagtgatcgg cccgttcgcc gataacgatc ttctttcttt agcacgcttt ttagcaatta
      961 atcttgatgg aattctgacg agagcgaaag aggtaagcca ggtcgtaccc gacttacctg
     1021 gaggagattt aatactcgag aatgccgtgc gcgacgggca aatagcgcag agagaaatag
     1081 agagtgtctt taccgcaaga ggaacgtttc gctaacagcg aggcgatctc tgcgtccggt
     1141 ttcggtttcg ctgtaaaggt tttgcctttg ccggcgacaa acagttcata aacaatgccg
     1201 cttgctgggg cggaactttg gcaatcggcg ctggcctgta gataacgttc acgctgttca
     1261 gcggtcagtt tatccacgcc gctgccatcc tgtacccaaa cattaacgcc ggttgcttta
     1321 acgtgttcca gcagttggcg atagccatcg ggcgacatgt ttccggcgaa aaaactactg
     1381 atataaaccg gttttgctga aacatcgcta atcagccgct gcgcgttgtt taaccatgtt
     1441 agcaaaggct gacgggcagc ttcgctgcgc cagttcaggt cgtcaatttc cgcgctgatg
     1501 taccagccat ccggcgttac gccaggcgcg gcgctccata atctggcttg ctggagatcg
     1561 gcagccagca ggcgattaag atagctttcc agcgctgcgg acgactgttt ctggtgcata
     1621 aaaaattccg gatcggcgtt cagcccgaca atcagcttca ggccagcctg ttgcgcagct
     1681 gcggcacgct taaacaataa cgcgcgctgt tctggctggg tgaatgcatc gccgtaacgg
     1741 gtccattgca aaaccagggt atcgaagcct tgcaaacgta actgactcat cagcccctgc
     1801 cactgggtat cggtaacctg actatctcgg ttttgtggtt gccagataat acctttcatc
     1861 gcaaaggaaa aagggctgac caaaagcagt gtcagcaata cgaaaatgaa cttacgcatt
     1921 taccagtgca ctccaatggt gagaaacgcg ttgttgcgct ctccgttacg ttgattaatc
     1981 gccttaaagg tatgttgata ctcgacgccg agactgactt tgtgcggcca ggcgtcgtag
     2041 tgcgtctcgc cggtccagat attccagcgg accccgactc cgccaagctg cgcgccctga
     2101 gtgcctttat cacgatagcc gttgtcctga acgtgagcgt aaggctcaat agtctgtccg
     2161 ttagctacct tctgatgcca gctgacgcga taatctgccg tccacgcctg aatatcctgg
     2221 cggatatatt gcgccgcatc gaggtacagg ttttgggcaa accagcctga accgttcggg
     2281 tgccattcgt cgctgtattt gccgccatta aagaatgagg cgctggcgcg cagcatggta
     2341 tcggatgcgc cattttgccc gttcagcggc aactgctgtt cgacggcgag gaaaaagatc
     2401 tgatcgcgca gcggcttcca gcgcagaccg gtgccggaca tcggattttt caccggcatc
     2461 atcaccccgt tttctccggt atcggcaaag acgcggctat aaactgagag caggtcgcct
     2521 tccagcagca tattgcgtcc gatgcggtac tcggcttcca gttgcccgta gctacgatag
     2581 cttttccctg gcgctgcgcc gccgacatta ttgttagcgg tgctcattgc cccggaacgc
     2641 aagccgatag aagaatcgaa actgaacgtc cagcggcgac cgacctcctc atgcaaacgg
     2701 cggaaattga agcgttgctg attttgctct ggggtcagtg gggttatcag cgcctgatta
     2761 tcaatgtcat caatcaccag ccgggcgtag tgctgcgtcg caggcatgtc atccagacgc
     2821 tggttcacgt aggccagttg tcggatcagt gccggatcgt ccggaagccc tttatgcgcc
     2881 tgttcgagca tttcccgcga ctgtgcgata tcaccgctat cccacaaggc gtaaccaagc
     2941 gctgcctgga tgttgctatt attcggttcc agttccagcg cggcgcgcaa atcactcacc
     3001 gccgccggga cattatgacg ttggcgataa attgtcgccc gcgcaacgta agcgttggca
     3061 gaaggcgcaa tattgattga gcgcgtgaga tcgctcagtg cgagttccgg ctgaccagga
     3121 atgtaacgtt gcgcatgcag cccccagtag agggcattgt ttcccagtcc acgttgttct
     3181 gcctgttgta gccagcgatc gcgagctgca ccatttcctg ccgcctgggc ggtattggca
     3241 gcagcaagca gatcctcatt gctcatgtcg tgaagactga ttttctgcca ggccgccagt
     3301 gcggtggcgt agtcctcaac ctgatacgcc tgataggcta ccgcacgatg ttgccaggcg
     3361 ttcggttgtc gttgttcggc ctgaagccat gcatacagcg ccacaccggg tagcgtgtcc
     3421 cgataacact ttgccagacg gttccaggcg gcagcatcgt aggaaggcga catatcgccc
     3481 agcaagcgaa ctattgccgg gcaattatct gcaatacccg gcaactgact ttgccactga
     3541 cgttgctccg ccagcggtaa gggtttcgat aaaatcgcca ccttcgccgg cgttgccagg
     3601 taaggatgac tttccagcag agacgccagt cgcgccatta aagtctggct gacacgcgca
     3661 tcgccctgga aaggatagcg ttgcagcaat aaatcggcag cttcgcgtga ctgctcgttc
     3721 tgcatcagtt gccaggtcag ttgatccagg cgggtaagat ttgccggttc ttgctgatac
     3781 agcaatcgtg ccagacgcag agattcagcc ttgttacggg tcgccacgct gacagcataa
     3841 cgctcctcaa gcatttcatt ggcggggagg gtggcgagca gtttttgcgc tgcgtcgtac
     3901 tgaccttctt ttaacagcac cggtagcgtc gcgccaacaa catactggcg gttatcggca
     3961 aactgcaccg tataattcgc caatgcctga acggggttgg cgctgtattt agataacaga
     4021 tagagccaac ttttctcttg tgcgtccgtg gtaaacagcg gcttattttc attgagataa
     4081 tgctggaggc gtgctttttc gccacgataa gccagcgcgg tcgcgtaagt aatatatgac
     4141 tgaggatcgg tgaagatccc ctgtgattgc agtgccagga tccgatcgtc cagctgcccg
     4201 gcaagaagca cgtcaaacca ctgacggcgt tctgccgcgc ttaatgtgtt ctgctggcgt
     4261 gcttcattgt atagcgtatc tgcctgggac cattgtttca ggtagattgc ccgttgcagc
     4321 agatcggttc gcagcgtttt tccttctggc gatgcagcaa acgtcgcatc gttcagttgc
     4381 gctctggcga caggtaattg tgccagccgc agggcattct gcccgacttc actgcgacaa
     4441 cgcagggtcg gcgcagcgtc gcacgctttt tgctgggcaa gcagttcttc aacagtcgta
     4501 acgcttttca cttcaaccgg aatagccgcc agactgcgct caagtcgggc atctcctggg
     4561 tgacgtttca gttgatcctc aagcaacagc cgcgcccggt catcatgacc aaaatggcga
     4621 taggcttccg caaggtataa agtcagcgga atattatccg gcacctgctg gtgtatatat
     4681 tcaaattcgc ggatggcggt tgcttcgtcg ttatttttct gtgccttcag cgccttatcg
     4741 agacggggat aaataacaaa atggcgataa tcgctcagcc ccagctcttc tgcgctggtg
     4801 ccgatattgt ctgcgagtgc gctggtactc aataaagacg tcagcagtaa accagaccat
     4861 ccgatgacgc gattaaggtt attctccttc attttcggac tccagttgcg caacctgttc
     4921 tgtgtttaaa cctgctttga gtaatagtga ttgcatcgaa acttgtaatt cgcgttgaat
     4981 tgtcaggacg cgatccaacg tttcctggct gataacgcct tcggtgacca aaaacttgcc
     5041 gagcggcaga gaactgcgtt catggcgcaa taacaacacg ttaattgctg aacgattaat
     5101 atgaccgagc gtggtcagta tttcggcgaa caggaactga tgcggcacat attgccgcca
     5161 gatttcaccg gtctgctgtt ccgtgagcca ctgatgctga accgcattgt acaacattgc
     5221 ccgcggatcg tgaccgcgtc ggcgtgcata ccagtgacgt aaccccgtga caatttgtcc
     5281 ccgcagaaca atgacgtaac gcactttgcg tccgacttta cgcgtcaggg ccgccagcga
     5341 aaccgggtca ataccatctt cactgccgac aattaactca tcattttcca gacgcagcgg
     5401 cagtactgca taatgcagcg ctacggaggc tggcatttcg gcaatcagcg aggacgggat
     5461 ctgccaggca tcgatggatt cccacgccac gccgttttgc tctgccagcg cctgtgccag
     5521 ctgctcggcg ctaatcagcc cctgcatcag cattgaaccg cccaggcgta gaccttcgac
     5581 gcgattacgc agtgctgtat cgagttgttc ttcagtgatg acctgatttt ccagcagaat
     5641 ttggcctaac gggcgcaacg agcgggtatc gccagtcacg ctggggaagt catgcgttgt
     5701 tttatcccac gcgacgcgac gtggatcgcc gtgttgaagt acctgtttta gcgcgcgcca
     5761 gttggccatg aagttaatca ggttgcccca gaaaagacgc aggacagata gcagcccctg
     5821 cgtcagaccg tagtagccag tgacgaaaat cacccgctgc acgatgcggt taaccatcaa
     5881 gccaaagttt agccacagca gggtcattaa ccatgcgctg ccgctaaaaa tagaaaggaa
     5941 atgccaggca ttgggccaca aactttcata cgccagcaac agcaaaagct ggagcatcac
     6001 cagcatcgcg aggaagctga caaagttact gattgcccct ttgcggtcgc gccagagaaa
     6061 gtagttcagc gtcaggctgg aggtccattt gtgggtttta aagccctgga aaacaatgcc
     6121 gatgatccag
//
LOCUS       DAFATA010000173         6093 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFATA010000173 DAFATA010000000
VERSION     DAFATA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.175"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1163)
                     /gene="ghxP"
                     /locus_tag="LGJ20_004546"
                     /gene_synonym="yjcD"
     CDS             complement(<1..1163)
                     /gene="ghxP"
                     /locus_tag="LGJ20_004546"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBK2388531.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDA"
     gene            complement(1709..2173)
                     /gene="soxR"
                     /locus_tag="LGJ20_004547"
     CDS             complement(1709..2173)
                     /gene="soxR"
                     /locus_tag="LGJ20_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBK2388532.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2259..2582
                     /gene="soxS"
                     /locus_tag="LGJ20_004548"
     CDS             2259..2582
                     /gene="soxS"
                     /locus_tag="LGJ20_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBK2388533.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(2585..4171)
                     /gene="pdeC"
                     /locus_tag="LGJ20_004549"
     CDS             complement(2585..4171)
                     /gene="pdeC"
                     /locus_tag="LGJ20_004549"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBK2388534.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            4601..4882
                     /locus_tag="LGJ20_004550"
     CDS             4601..4882
                     /locus_tag="LGJ20_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBK2388535.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(4981..5517)
                     /gene="ssb1"
                     /locus_tag="LGJ20_004551"
     CDS             complement(4981..5517)
                     /gene="ssb1"
                     /locus_tag="LGJ20_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBK2388536.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            5771..>6093
                     /locus_tag="LGJ20_004552"
     CDS             5771..>6093
                     /locus_tag="LGJ20_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK2388537.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDY"
BASE COUNT         1629 a         1596 c         1403 g         1465 t
ORIGIN      
        1 gcatcaacaa aatcagcaaa gtcgattttc gccacgttgc tcagcatcag caggccaacg
       61 tacatcagcg ccggagccgt tgcatacccc ggaacgaggt aagagagcgg agagaggaac
      121 agaatcagca ggaacagcac gccaacggtg atagccgtca aaccggtttt accgcccgcc
      181 gccgtacccg ccgcagactc gatgtatacc gctgccggag ctgcacccac caggccagag
      241 aaaacgctgc tcatggagtc agtggtcagt gctttcccac cgtcgatgat ctgcccatct
      301 ttatccagca ggttcgcctg gccggcgacg gcacggatag ttccggtggc atcaaatact
      361 gccgtcatca ccagcgccag aacgcttggc aggactacag gattcagcgc gcccataatg
      421 tccaggctgc caatcagtga attgccgttt tcatcgctca atgaaggcat ggcgaaaacg
      481 ccggagaaat ggacgttagg atcgaagatc aaaccgacaa ttgagatacc gataatggtc
      541 agcagaatgc caccagggac tttcagtttt tccaggccga tgatcaccgc cagacctacc
      601 agtgacataa tcaccgggaa ggtcgcgaaa tcacccagcg caacgggcag accatcaagc
      661 gggtttttaa tcaccagacc gacaccatta gcggcaatga gcagcaggaa cagaccgata
      721 ccgatccccg tgccgtgcgc cacaccgtga ggcaagttgc gcaaaatcca gctacggata
      781 cccgtggcag aaattaccgt aaacagcaca cccatcagga acacggcacc cagcgcgaca
      841 ggtacgctaa tatgttgccc cagcaccagg ctgaatgcgg taaatgccgt cagggagatg
      901 gcgcaaccaa tcgccaacgg cagattagcc cacagaccca tcacgataga accgagtccg
      961 gcaaccagac aggttgcaac gaaaactgcc gcaggcggga agcctgcttt acccaacata
     1021 cctggaacga cgatgaccga gtagaccatc gccagaaacg ttgttaaccc ggcaaccact
     1081 tcctgacgga cagtgcttcc acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg
     1141 gtacgcgctg atggcgtaga cataagaaaa catcccctga gagtttaatt ttcgtcaaga
     1201 tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc ctgctccagg tttgcgacaa
     1261 tttgctgcgt cgcaaaaaag caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa
     1321 aagcaaacga ttatccggcg atcatagcgg ctttttcaac cgaagtttgc tgcttttttc
     1381 tcttttattg tcttgacgat gaaaaaccag gcaattgatg cgcaaacgtt atcgtcggtg
     1441 cgcaagattg cagcgttatc gagccgtata ccgttcagga ttgcgataaa aaatcgtctg
     1501 agatgacaaa aatattgcgg ttaaaaagta tacttttaca ctggcgaaga aacgtcggtg
     1561 acatacaatt aaagcatcaa caccaaccgg aacctccacc acgtgctcga atgaggtgtg
     1621 ttgacgtcgg gggaaaccct cctgtgtacc agcgggatag agagaaagac aaagaccgga
     1681 aaacaaacta aagcgccctt gtggcgcttt agttttgttc atcttccagc aagcgtgcgc
     1741 cggtaccttc ttctcctaag cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa
     1801 ggcagccaca accaatacat ccgtccagtt cgtcacgcag cgctactaag gtatgaatgc
     1861 gccgatccaa ctcttctcgc cattgggacg aaagctgttt ccactctttc gcacttaacg
     1921 tatgcccttc gggcaacacg ccaaacgctt caccaatggt cgccagcgga atgccaatac
     1981 gctgagcaat tttgataatt gcaacatatc gcaacacatc acgtttatat cgccgctgat
     2041 tgccgctgtt acggatactg gtaatcaacc ctttactttc atagaaatgc agcgccgata
     2101 ccgccacacc gctgcgtttc gccacttcgc cgggggttag cagcgcttta atgcggggta
     2161 atttcttttc cataaatcgc tttacctcaa gttaacttga ggaattatac tccccaacag
     2221 atgaattaac gaactgaaca ctgaaaagag gcagatttat gtcccatcag aaaattattc
     2281 aggatcttat cgcatggatt gacgagcata ttgaccagcc gcttaacatt gatgtagtcg
     2341 caaaaaaatc aggctattca aagtggtact tgcaacgaat gttccgcacg gtgacgcatc
     2401 agacgcttgg cgattacatt cgccaacgtc gcctgttact ggccgccgtt gagttgcgca
     2461 ccaccgagcg tccgattttt gatatcgcaa tggacctggg ttatgtctcg cagcagacct
     2521 tctcccgcgt tttccgtcgg cagtttgatc gcactcccag cgattatcgc caccgcctgt
     2581 aattttattg cccgcgcgtt aactcccgcg cgggtaattg ctccatccat tgcataaaca
     2641 cctgcggcgg catcgccttc gcaaagaacc atccctggca atagcgcacg ccgcgtttgc
     2701 gcagccagtt aacctgctcc tcagtttcga cgccttcagc gatcgttttt aaccccaggc
     2761 tgtgcgccag ctcgatgatg tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg
     2821 tttcaacaaa cgatttgtcg attttcaaaa tatcgacatt caatgattta aggttatgca
     2881 agttagagta gccaatacca aaatcatcaa ttgccacttc gtaaccagcc tggcggaaag
     2941 ccagaataat cggcgtcatt ttttcgacat caagaaatgc atgctcagtc acttcaaatt
     3001 taatttgctg cggacgcacc gcgtattgct ctgttttctg attgattcgc gctatcaacc
     3061 gtgacgtatg aaaatcggag gccgacaggt taatagaaac atagcgatct gcatgtgttg
     3121 ccaggtaatc gcccagatcg cggaagacat tatcaataac ataatcagtt atctgttcta
     3181 tcatcccctc cttttctgcc agcggaataa actctgccgg attcattatt tgcccctgct
     3241 caccaagcca acgtaacaac gcttcagcgc cgatacattt ttctgttttg atatcgatta
     3301 ttggctggta ataaagacaa agttgatgtt tttcgagggc gcgttgcaat ttacgtttgg
     3361 gagataaata gttttgtcga atacgtagcc agagtaatag cagaacaaga ctccccaaaa
     3421 taccggcggg caacgcaaaa ataagatgat tataaaaatg ggttataaga cgttgatatg
     3481 aagtcgcaac aatggctgca attgggcgtt ttgtcgaata aactgtcgca tataaatagc
     3541 catttctttg tacggttaaa tccttcaaat gaatcagcgg agaaaacgtt gctgccgagg
     3601 cctctttgct taacgagaaa aaggttttcg tcaccgtatc atacacaccc cattgcaatg
     3661 tcggatcatc agacatcact tcactccaga agagagggtt gataaccgcc acataatttc
     3721 cccgctgcat ataggtcatt ttatagccag agaaaaaagg cgtatcgcgg taataataga
     3781 tagaaacgtt aggttcacgc ttataatcgg ccggtgcaat cgtatagccg tttacaggcg
     3841 ctatcagcga tgagcataaa aaatggttat cacgggcata gatcaattca ttaatataaa
     3901 gatagccacg aataatattc aacattcgct tttgatgggc tggagtgcaa acttgcccct
     3961 gatagcgttc agctgcatcg ctcactaaat ctgcctggcg aatgaccagc tcggatttat
     4021 ccagtgccag ttgagcaaag gttcggagct gattattcac ttctgatttt gcccaaagga
     4081 aggcaatcca tagcgaaaga atgatgggaa agagaactaa aaagataatg cccggcaacg
     4141 ccagtaattg gtgtcgtgca cgatgactca ttgctatcaa tttcctgcct gtttataccc
     4201 ggccattgca aggtgaaaac cctgggtacc gacgtcttta gataaatctt acggtataaa
     4261 tagcactttt gcctgatttg aaattgatac ctttctaatc gtcacctgga tgaaaataat
     4321 tcattgatat tcaaaattaa tttcctacag gaatgcagag gcggcgggaa gatatgccct
     4381 taatgccact gaaaaaccac ataatctgta cagcaataaa agtcacggcc taatatggct
     4441 aacaaacaat caattgtgat atagttcaca aaattaatga aacaaacaga gtgtttcatt
     4501 tttgtgttct gcaagccccg cgatagccat agtgtaccac gcctcccgtg aacaacgccg
     4561 cgctgtccag ggcatcggct cttttcagag gaattgtttg atggctaccc tcaccactgg
     4621 cgtggttctt cttcgctggc aacttcttag tgccgtaatg atgtttctgg ccagcacact
     4681 caacatccgt tttcgtcggt ctgattatgt cgggcttgcg gtgatcagca gcggtctggg
     4741 cgttgtttcc gcctgttggt tcgcaatggg gttgcttggc atcacaatgg cggacatcac
     4801 cgctatctgg cacaacatcg agtcggtgat gatagaagag atgaatcaga caccgccaca
     4861 atggccaatg attttgacct gatacgtaga agcctccata acggaggctt cttttttatg
     4921 gctggcagat gctttaatca tccaccttaa aacaatataa cctattgttt taatgacaaa
     4981 tcagaacgga atgtcatcat caaagtccat cggcggctcg ttagacggcg ctgccggagc
     5041 ggactgctgc gggcgagact gcgcgccgcc gctgaactga ttgccaccct gcggctgctg
     5101 tggctgaccc caaccgccct gcggctgacc accaccgata ttgccacctg ccggagcgcc
     5161 accaccctga cgaccaccca gcatctgcat ggtgccgcca acgttcacca cgacttctgt
     5221 ggtgtagcga tcctgaccgg attgatcggt ccatttacgg gtacgcagct gaccttcgat
     5281 ataaacctga gaacctttac gcagatattc gctcgccact tctgccagtt tgccgaacag
     5341 cacaacgcgg tgccattcag tctgttcttt catctcgccg gtcgctttat cacgccagga
     5401 ttcggaagta gccagcgtaa tgttggcaac tgcgccacca tttggcatgt agcgtacttc
     5461 cgggtcctga cccagattac caacgagaat aaccttgttt acgcctctgc tggccatgtt
     5521 cgtgtctcct gaaaaaaatc gttctgaata agtgtaaacg cgcgattgta ccattaccaa
     5581 tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca gacattgtta
     5641 cacaacactc cgggtaatgc attccaatac tgtatattca ttcaggtcaa tttgtgtcat
     5701 aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg
     5761 ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc tcaaaaacat
     5821 caacctcgtt atcccccgcg acaagctcat tgtcgtgacc gggctttcgg gttctggcaa
     5881 atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg ttgaatccct
     5941 ttccgcctac gcgcggcagt ttctgtcact gatggaaaag ccggacgtcg atcatattga
     6001 ggggctttct cctgccatct caattgagca gaaatcgacg tctcataacc cgcgttctac
     6061 ggtggggaca atcaccgaaa tccacgacta ttt
//
LOCUS       DAFATA010000174         5979 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFATA010000174 DAFATA010000000
VERSION     DAFATA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.176"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            248..631
                     /gene="secE"
                     /locus_tag="LGJ20_004553"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="LGJ20_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK2388538.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="LGJ20_004554"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="LGJ20_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK2388539.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="LGJ20_004555"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="LGJ20_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK2388540.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="LGJ20_004556"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="LGJ20_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK2388541.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="LGJ20_004557"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="LGJ20_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK2388542.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="LGJ20_004558"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="LGJ20_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK2388543.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..>5979
                     /gene="rpoB"
                     /locus_tag="LGJ20_004559"
     CDS             4135..>5979
                     /gene="rpoB"
                     /locus_tag="LGJ20_004559"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK2388544.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYV"
BASE COUNT         1510 a         1439 c         1573 g         1457 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtt
//
LOCUS       DAFATA010000175         5976 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFATA010000175 DAFATA010000000
VERSION     DAFATA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.177"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..590
                     /locus_tag="LGJ20_004560"
     CDS             <1..590
                     /locus_tag="LGJ20_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBK2388545.1"
                     /translation="YITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLRGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            562..945
                     /locus_tag="LGJ20_004561"
     CDS             562..945
                     /locus_tag="LGJ20_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HBK2388546.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1050..1892
                     /locus_tag="LGJ20_004562"
     CDS             1050..1892
                     /locus_tag="LGJ20_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBK2388547.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNQIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1920..2878
                     /locus_tag="LGJ20_004563"
                     /pseudo
     CDS             1920..2878
                     /locus_tag="LGJ20_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2890..3351
                     /locus_tag="LGJ20_004564"
     CDS             2890..3351
                     /locus_tag="LGJ20_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBK2388548.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            4058..4495
                     /locus_tag="LGJ20_004565"
     CDS             4058..4495
                     /locus_tag="LGJ20_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388549.1"
                     /translation="MADKAILWALISASNKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(4954..>5976)
                     /locus_tag="LGJ20_004566"
     CDS             complement(4954..>5976)
                     /locus_tag="LGJ20_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2388550.1"
                     /translation="LVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNN
                     QLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDR
                     LFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNG
                     EQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQ
                     KRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQS
                     LTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMH
                     YLYLDH"
BASE COUNT         1924 a         1102 c         1311 g         1639 t
ORIGIN      
        1 gatatatcac tcaggatccg attggactga aggggggatg gaacctgtat ggatatcaat
       61 tgaatccgat atcagacatc gaccccctgg gtttatctat gtgggaggat gcaaaatcgg
      121 gggcatgtac taatggtctt cgcggcacac tatccgctat gataggtcca gataaatttg
      181 attctataga tagcaccgca tatgacgcct taaataaaat aaatagccaa tctatttgcg
      241 aagataaaga gttcgctggt ttaatatgta aggataatag tggcagatat ttctcaacag
      301 cacctaaccg aggagaaaga aaaggatcat atccattcaa tagcccttgc cctaatggta
      361 ctgagaaagt atcagcttat catactcatg gtgcagatag tcatggagaa tattgggacg
      421 aaatattttc aggtaaagat gagaaaatag ttaaaagtaa agataacaat atcaagtcat
      481 tttatttagg tacgcccagt ggtaatttta aagcaataga taaccacggg aaggaaataa
      541 caaacagaaa aggattacct aatgtctgca gagttcatgg taatatgtaa aaaaatattg
      601 tttaggaact gtgtcattgt atctttgttt gtttttacat acaacacatg ggcgcagtgt
      661 aataataata ttaaaataat gcgcaagtat gaaagtgaag gtaaatatac cgttagaaat
      721 ttggttaaaa ataaagctat agcattggaa ttagctgaga tatatgttaa gaatcgttat
      781 ggacaggatg ccgcagaaga agaaaaacca tacgaaatca ctgagttaac aacaagttgg
      841 gttgttgaag gtaccattca ctcagaccaa attgctggtg gggtttttat tatagaaata
      901 ggcaaaaatg atgggagaat tctgaatttt ggccacggaa aataagtatg taaggataat
      961 caaacatcgt gtgcgctgat ggcagagtag gtggaggact ccagacagtc aaacgataga
     1021 aaaagatagc ctttatggag gttcctgcaa tgtcaaatac ataccagaaa agaaaggcaa
     1081 gtaaagagta tggtttatat aatcaatgta agaaactaaa tgatgatgaa ttatttcgct
     1141 tacttgatga tcacaattcc ttgaaaagga tttcatctgc cagagtatta cagttaagag
     1201 gtgggcaaga cgctgttaga ttggcaattg agttctgctc tgataaaaat tatatccgta
     1261 gagatatcgg agcatttata ctcgggcaaa taaaaatttg caaaaaatgc gaagataatg
     1321 tttttaatat tttgaacaat atggcattga atgataagag cgcttgcgtt cgagctacgg
     1381 caatcgagtc aacggctcag cgatgcaaga aaaaccaaat ttattcacct aaaatagtag
     1441 aacaatctca aattactgct tttgataaat cgactaatgt cagacgtgct acagcatttg
     1501 ctatttctgt tatcaatgat aaagcaacaa ttccactatt gattaatctg ttaaaagatc
     1561 caaatggaga tgtcagaaac tgggccgcat ttgcaataaa tatcaataaa tatgataata
     1621 gtgatattag ggattgtttt gtggagatgc ttcaggataa aaatgaggaa gtccgtattg
     1681 aagcaataat cggactttcc tacagaaaag ataaaagggt tttatctgtt ttatgcgatg
     1741 agttaaaaaa aaatactgtt tatgatgata tcattgaagc tgcgggtgaa ttaggtgata
     1801 aaacgctact tcctgtttta gatactatgt tgtacaagtt tgatgacaat gaaattataa
     1861 cttccgctat tgataagctg aagcgttcat agcgtgagtt gcctatgcac agtgggggat
     1921 tcccgccggc acggtgcaga tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt
     1981 gagtgaggaa agccgccgct ggctggcatc gtgcggcctg accgtggagc agatgaaaaa
     2041 ccagatggac ccggtgtaca cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg
     2101 cggcctgccg ctggcgctca tcagcacgga aggggcaaca gcgtggtgcg cagaatatga
     2161 tgaatggggc aacctgctga gtgatgagaa cccgcatcat ctgcagcagc tcattcgcct
     2221 gccgggtcag cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga
     2281 cccgctgcag gggcggtata tcactcagga tccgattggg ctgaaggggg gatggaattt
     2341 ttatcagtat ccgttgaatc cggtcataaa tgtagatccg caaggtttgg ttgatataaa
     2401 tttatacccc gaaagtgatc ttatccattc tgtagctgat gagattaata tcccaggcgt
     2461 tttcacaatc ggggggcatg gtacccccac atctattgaa tccgcaacgc gcagtatcat
     2521 gacagctaaa gatctagcat atctaattaa atttgatggg aattataaag atgggatgac
     2581 agtttggtta ttttcttgta atacaggtaa aggacaaaat tcatttgcta gccaattagc
     2641 taaagagtta catacaaatg taataggacc tgacacgcta tggacgtggt gggggcgagg
     2701 aactaatggt aagttaaaaa tggatacagt gctaacagca ccaacgaacc ttaattcaaa
     2761 taaggatcta atggctataa caacaaaaga ccttggtaat tggataacat atgggccatc
     2821 tgggcacccc atttctaata tgcaaggtac gccagaaaaa cccagtgata taagataggt
     2881 tgtagatgta tgaaagcatg cttgttacta tttttttatt tttcttttat ttgtcaattg
     2941 catggtgctg atgtgaaaat aaaagaaaac gaaagtgtga tgggatctac agcaatgacc
     3001 tatgatttaa gtgaagaaaa gctgatgaaa ctgaagtata aatcacaaca tggtgattca
     3061 gaggcatcat ttcgactata tcaatattac tgctttacta aaaataatat tgataaacaa
     3121 ttgcgattct tggaaagatc agcatctcag gggaatgtta cagcgcagtt taattatggg
     3181 gtctttttat cagatacaaa tccaacatta tcagaatatt ataatttgaa tagagctatt
     3241 tattggatgg aattcgctgt aaataacggt aatattgatg caaagagcaa acttcaagag
     3301 ctcaaaaagc taaagcgaat ggatagaagg aagaataagg agaatccgta aaagcaatag
     3361 ttcatcagac tgccgcagca gtagtatgat gatgaatcag ggctgtacta taactattat
     3421 cggtaacatt aaccgcagca ggggcggtat aacactcagg ctccgattgg gctggaaggt
     3481 aggtggaatt tttatcagta tccgttgaac ccgatttctg gaatcgatcc ccttgggatt
     3541 agccacctgt ttatacagta taactttagg tatgctttcg tgtgtttcag atataccatg
     3601 tgatgataat ttttatgatg tgcttagtat tccagtcgct tctggtaata atatgcaatg
     3661 taaaaataat ccccgttgta cacatttata aatagatggc caatacctca aggcctatgg
     3721 agttggaatg ttaatgaccc gggagcaagc aatagaaaac ctgacggcat acgattagta
     3781 ccgtcagtta atacaggaac ttataatcgc aatggatttt caatacatag ttgtttaaac
     3841 gcttttggtc cgtctttagg acctcgattc tgttccgagg gttgtataac tggtttatct
     3901 aatgacatgc aaaaattaaa tgagttaata ttttcagagc cagatagcac attaacagtt
     3961 acggactaaa aaatatggca aggttattaa caattgtatt tttttctcta tttctgcgag
     4021 tgcagttact gcaagacaat ttcagagaga gttagatttg gcagacaaag caatattatg
     4081 ggcactaata tcagccagta ataaagaggg acgaaaagcg tgttcactaa gctattttgc
     4141 ctgtaaggcc gcagaggctg aactcggact ggcatatatg gctgcgaatg ataacaagga
     4201 atttctcaca tccttatcta atataatgag gtacaaaata gatgctggac tttccgaatc
     4261 ctatacatgc tatttattaa gtaagggaaa gatcataaga ccatatctga aaaatctaaa
     4321 tccactccaa cttgccgctg attgcattga aacagtaaat aaaataaaag ataagaataa
     4381 aaaaatcatt gatataaatt cagttaatat ttgtagtgat gataaaaata taaaattgag
     4441 agttaatagt actattatgg ccattgatga ttctataaag tgcatcgatg aatgataact
     4501 taattttatt taaaaatata ttaataactc actctccagg agttttttaa cttagtttta
     4561 taatgaatac gataattaaa agtgaagaag aaaatagttg tatacatata ttccatgacc
     4621 ctacattgtg atctatagat taaatatttt ttaaatgtga ctgtcatcct tcagataata
     4681 aagccatcat cgcaatagat ggaaaagcac tcctgctctt ttttgattag agccgtcgca
     4741 ggaggaacaa ctcatatcat cattgcgatc tcgacgatgt atagtctcat cctgcggcag
     4801 aacaagacgg ataaaaaatc caatgagagc acactcatcc agaacttatt aacataatga
     4861 atattaaaga aaaatcatcg cagctaatga agtaatatgc aaaaataatt agtccttaca
     4921 aggagcaaag ttccataatc tcccccctcc ccctcaatga tccaaataaa gataatgcat
     4981 ccagctggtc attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga
     5041 gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttaggatc
     5101 cagttcaatg gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac
     5161 cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atactagtca gtttgccgtg
     5221 aaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccgg gtttcagacg
     5281 taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaagac
     5341 catcaccgga cgcagcggca aggcagctgc gtatgtacct gggcggatca gtacctgagt
     5401 aacgtagcca ttgctcggcg cgcgaatgac agtctgctca aggttatatt ttgcttcagt
     5461 tagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg
     5521 gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcgaggaa
     5581 attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc
     5641 tttcaagtaa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt
     5701 gttggcctgc gcttcagtga gctgcgcacg cagcgtcttt atattatgcg tcgccgtcat
     5761 caggtcagcc tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag
     5821 cacctcgccc ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt
     5881 cacctgtggc gtgataggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg
     5941 gttgtagttc atcaacaaaa tcaaaccact caccag
//
LOCUS       DAFATA010000176         5953 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFATA010000176 DAFATA010000000
VERSION     DAFATA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.178"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..586
                     /gene="rbsC"
                     /locus_tag="LGJ20_004567"
     CDS             <1..586
                     /gene="rbsC"
                     /locus_tag="LGJ20_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBK2388551.1"
                     /translation="IATLVMMLLLRGVTMVYTNGSPVNTGFTENADLFGWFGIGRPLG
                     VPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNKIKIIVYSLCGL
                     LASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGTLIGALILGF
                     LNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            611..1501
                     /gene="rbsB"
                     /locus_tag="LGJ20_004568"
     CDS             611..1501
                     /gene="rbsB"
                     /locus_tag="LGJ20_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBK2388552.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            1627..2556
                     /gene="rbsK"
                     /locus_tag="LGJ20_004569"
     CDS             1627..2556
                     /gene="rbsK"
                     /locus_tag="LGJ20_004569"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK2388553.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKSIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            2560..3552
                     /gene="rbsR"
                     /locus_tag="LGJ20_004570"
     CDS             2560..3552
                     /gene="rbsR"
                     /locus_tag="LGJ20_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBK2388554.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAISAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3518..4945)
                     /gene="mdtD"
                     /locus_tag="LGJ20_004571"
     CDS             complement(3518..4945)
                     /gene="mdtD"
                     /locus_tag="LGJ20_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBK2388555.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(4968..5660)
                     /locus_tag="LGJ20_004572"
     CDS             complement(4968..5660)
                     /locus_tag="LGJ20_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK2388556.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1527 a         1456 c         1557 g         1413 t
ORIGIN      
        1 tatcgctacg ctggttatga tgcttttact gcgcggcgtg accatggttt ataccaacgg
       61 tagcccagtg aataccggct ttactgagaa cgccgatctg tttggctggt ttggtattgg
      121 tcgtccgctg ggcgtaccga cgccagtctg gatcatgggg attgtcttcc tcgcggcctg
      181 gtacatgctg catcacacgc gtctggggcg ttacatctac gcgctgggcg gcaacgaagc
      241 ggcaacgcgt ctttctggta tcaacgtcaa taaaatcaaa atcatcgtct attctctttg
      301 tggtctgctg gcatcgctgg ccgggatcat tgaagtggcg cgtctctcct ccgcacaacc
      361 cacggcgggg actggctatg agctggatgc tattgctgcg gtggttctgg gcggtacgag
      421 tctggcgggc ggaaaaggtc gcattgttgg gacgttgatc ggcgcattaa ttcttggctt
      481 ccttaataat ggattgaatt tgttaggtgt ttcctcctat taccagatga tcgtcaaagc
      541 ggtggtgatt ttgctggcgg tgctggtaga caacaaaaag cagtaataac gactacagga
      601 catcttgaat atgaacatga aaaaactggc taccctggtt tccgctgttg cgctaagcgc
      661 caccgtcagt gcgaatgcga tggcaaaaga caccatcgcg ctggtggtct ccacgcttaa
      721 caacccgttc tttgtatcgc tgaaagatgg cgcgcagaaa gaggcggata agcttggcta
      781 taacctggtg gtgctggact cccagaacaa cccggcgaaa gagctggcga acgtgcagga
      841 cttaaccgtt cgcggcacca aaattctgct gattaacccg accgactccg acgcagtagg
      901 taatgctgtg aagatggcta accaggcgaa catcccggtt atcactcttg accgccaggc
      961 aacgaaaggt gaagtggtga gccacattgc ttctgataac gtactgggtg gcaaaatcgc
     1021 cggtgattac atcgcgaaga aagcgggtga aggtgccaaa gttatcgagc tgcaaggcat
     1081 tgctggtaca tccgcagccc gtgaacgtgg cgaaggcttc cagcaggccg ttgctgctca
     1141 caagtttaat gttcttgcca gccagccagc agattttgat cgcactaaag gtttgaacgt
     1201 aatgcagaac ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga
     1261 aatggcgctg ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt
     1321 cggatttgac ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac
     1381 tatcgctcag ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct
     1441 gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta
     1501 gttttaatca ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg
     1561 taatataaag aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc
     1621 ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt
     1681 cttaatcttc aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt
     1741 gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc
     1801 gcgtttattg cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc
     1861 actgataaca ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg
     1921 ctgatttttg ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct
     1981 gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta
     2041 ttaatgcagc tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa
     2101 aataagacta tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg
     2161 gcgctggtgg acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt
     2221 gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aagtatccgt
     2281 actgtactga ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag
     2341 cgcgttcctg gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac
     2401 ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc
     2461 catgctgccg ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt
     2521 gaagagatcg acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt
     2581 gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc
     2641 gtcagtgaag cgatttccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca
     2701 tcagccctgg cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact
     2761 gccagtacca atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa
     2821 cgcggttata gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat
     2881 ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat
     2941 caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg
     3001 gctccgttcg atggcgacag cgatcttatt caggataact cgttgctggg cggagactta
     3061 gcaacgcaat atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg
     3121 gataaaactc cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt
     3181 ctcagcattc ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac
     3241 gccatgcgcc aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac
     3301 gctatggctg ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat
     3361 atcgcggtga ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc
     3421 actatccacc aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg
     3481 ataacccagc cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc
     3541 ggttcggctt agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa
     3601 gcatgaacat tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga
     3661 attgttcgac agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta
     3721 cagcaacgcc taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc
     3781 tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg
     3841 acatagccat ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg
     3901 attgcaaaga gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat
     3961 ggcgatagcc cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta
     4021 acgctgtcgg tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta
     4081 acatcaatgg cataaggaac ggtacacaac cggtccccag acgggttgca atattgccta
     4141 cgataccgat cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg
     4201 tgtgtcgcgc atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca
     4261 aggcaatcca gctggcgaca atcctttccc cgaatagctc tattccgctt gagaagagaa
     4321 caaggctgag gccaaacagc aaaaagccag tgatatcgaa tctgcgtcgt gcggtggtga
     4381 aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta
     4441 aaaatatcca gtgccaggtt gcccaggtga ccagcacgcc gccaagaacg gggcctaaaa
     4501 ttggccccac cagacccggc atggcgacaa aattcaatac tggaagaagt tcattacgag
     4561 gataagcgcg cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct
     4621 gaataacccg gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac
     4681 ccaatgtgaa cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta
     4741 gccatccgct taccggaata agcatcgcca ccgtcagcgt ataactgatg atggctgatt
     4801 gcatcgcgag aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa
     4861 tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca
     4921 tactgcgctt ctttttatcg ctcattcaat gtcctgttat cgggttgtca cttatcaggt
     4981 gagcgtagca gcgcctgaca agctttaaat gccgcgtcgc catcgctttg gacaatcgca
     5041 tcgacaatcg cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag
     5101 taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc
     5161 atttcataga tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag
     5221 ttctctttta atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg
     5281 gtgccaaccg ttgcagccag taggcaagct tgcggctcca gacagatacg cataaccaga
     5341 aagtgatcga tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga
     5401 tcaagaaaat tccagtttga ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa
     5461 accatccctt ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca
     5521 aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt
     5581 aagatgcgtt gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac
     5641 tgttgtgcgc ttaaaggcat tacttatctt cctttttctt tttattcctc cttagtatgc
     5701 caccaggaag tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa
     5761 cggggctttt gctgaaaaaa tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
     5821 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg
     5881 ggtcagcggg gttctcctga gaattccgac agagaaagca aaaataaatg cttgactctg
     5941 tagcgggaaa gcg
//
LOCUS       DAFATA010000177         5949 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFATA010000177 DAFATA010000000
VERSION     DAFATA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.179"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..332
                     /locus_tag="LGJ20_004573"
     CDS             <1..332
                     /locus_tag="LGJ20_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2884 family protein"
                     /protein_id="HBK2388557.1"
                     /translation="MNRIIETRSDGLTFHYKAIDQVRAEGQQLVNQAMGGILQDSINE
                     MGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGKDVCSRVVTLEDSRK
                     ALVGNLK"
     gene            508..1554
                     /gene="ansB"
                     /locus_tag="LGJ20_004574"
     CDS             508..1554
                     /gene="ansB"
                     /locus_tag="LGJ20_004574"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBK2388558.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            1671..2678
                     /locus_tag="LGJ20_004575"
     CDS             1671..2678
                     /locus_tag="LGJ20_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBK2388559.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(2833..3969)
                     /gene="hemW"
                     /locus_tag="LGJ20_004576"
     CDS             complement(2833..3969)
                     /gene="hemW"
                     /locus_tag="LGJ20_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBK2388560.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(3962..4555)
                     /locus_tag="LGJ20_004577"
     CDS             complement(3962..4555)
                     /locus_tag="LGJ20_004577"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBK2388561.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(4563..4853)
                     /locus_tag="LGJ20_004578"
     CDS             complement(4563..4853)
                     /locus_tag="LGJ20_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBK2388562.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(4850..5416)
                     /gene="yggT"
                     /locus_tag="LGJ20_004579"
     CDS             complement(4850..5416)
                     /gene="yggT"
                     /locus_tag="LGJ20_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBK2388563.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(5434..>5949)
                     /gene="yggS"
                     /locus_tag="LGJ20_004580"
     CDS             complement(5434..>5949)
                     /gene="yggS"
                     /locus_tag="LGJ20_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBK2388564.1"
                     /translation="DKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHTIDRLR
                     IATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRLRGLMA
                     IPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTMVRIGT
                     AIFGARDYSKK"
BASE COUNT         1583 a         1505 c         1496 g         1365 t
ORIGIN      
        1 agatgaaccg cattatcgaa acgcgcagcg atggcctgac gtttcactat aaagccattg
       61 atcaggttcg cgccgaaggc cagcaattag tgaatcaggc aatgggtgga attttacagg
      121 acagcattaa tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga
      181 atgtgctggg aagcctgggg gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg
      241 aaaaagattt ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata
      301 gccgcaaagc cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt
      361 ttttatgccg tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat
      421 atattcacgt tgtaaattgt ttaacgtcaa atttcccata caaagctaag ggataatgcg
      481 tagcgttcac gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca
      541 ctggttatgg gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc
      601 ggcgggacca ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa
      661 gttggcgtag aaaatctggt taatgcggtg ccgcaactga aggacattgc gaacgttaaa
      721 ggtgagcagg tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg
      781 gcgaaaaaaa ttaacaccga ctgcgataaa accgacggct tcgtcattac ccacggtacc
      841 gacacgatgg aagaaaccgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg
      901 gtgatggtcg gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg
      961 tataacgcgg tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg
     1021 atgaacgaca ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg
     1081 accttcaagt ctgttaacta cggtcctctg ggctacattc acaacggtaa gattgactac
     1141 caacgtaccc cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat
     1201 gagctgccga aagtcggcat cgtttataac tacgctaacg catccgatct tccggctaaa
     1261 gcactggtag atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg
     1321 tataaatccg tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt
     1381 tcttcccgcg taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac
     1441 ggcttcgtcg cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct
     1501 ctgacgcaaa ccaaagatcc gcagcagatc cagcagatct tcaatcagta ctaatcacct
     1561 cgccccggta tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat
     1621 taccctaatg ataatcaccg gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc
     1681 aatggattgt tggcacggca ctgcttatgt tgatgactgg taatgcttgg gcagatggcg
     1741 aaccgccaac tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca
     1801 agcttgatgc tatcacctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag
     1861 cggaaggcga tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt
     1921 acgaactgct cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa
     1981 ccagtaaagg aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag
     2041 ccagcgatcg tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga
     2101 atagcgaaga tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga
     2161 actcgattcc tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc
     2221 agaaagcagc ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag
     2281 aagcctttaa aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg
     2341 cgttcgcggt taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac
     2401 agagtgaaga gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac
     2461 aacggcgaca gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg
     2521 taacgctcga aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa
     2581 aagtcgcgat tttgatgaaa attgacgata tcaaccaagc taatgaacgc tggaaaaaag
     2641 ataccgaaca actgcggcgc aatggggtga ttaagtaagt ttgatatagt gcacgatttg
     2701 tagcaggatg cggcgttgac cccgcatccg gcaatcaacg tctgatgcga cgcttgcgcg
     2761 tcttatcaga cctacaaccg ttgccacatg taggctggat aaggcgttca cgccgcatcc
     2821 ggcaatacaa gtttactcag ccagaaaaag ctccagcagc gaatttaaaa acagcttccc
     2881 atgttccgtt atctgccagt aatccgcaca ttcggtgaga taaccctggg caatcgcctc
     2941 gtctaactgt gggcgaatca catcttcgca aagcccggta tacgcaataa actccacgcg
     3001 cggcgcggcc tccagcagac ggaagcgatt cataaagaac tcaaacggct tatctgcggc
     3061 ttcgacatca cgctggcttt ccagatacct tccctgcata aaaccacgcg gatgacgcgt
     3121 tttggtggta cgcagaatgc gcccatccgg gaaggtcact ttgccgtgcg cgccacagcc
     3181 aataccgatg tagtcgccaa atcgccagta gttgagattg tgctggcact gataaccggg
     3241 tttggcgtaa gcggaagttt catattgctg ataacccgct gcggttaata actgatgccc
     3301 ctgttcgaaa atatcccaca gcgcgtcatc gtccggcagc accggtggtc gcgaaccaaa
     3361 cagcgtattg ggttcgatgg tcagttgata ccaggaaaga tgcggcggat tcagttcaat
     3421 ggcctggcgt agatcgccaa gcgcctcttc cagtgattga tccggtagtc catgcatcaa
     3481 atcaaggtta aagctacgta accctaaacc gctcgccagc tttgccgctc gtttcgcttc
     3541 ttgcgggcca tgaatgcgcc cgagtcgttt cagcttttct tcgctaaaac tctgcacacc
     3601 aatagagatg cggttcacac cagcacgctg ataatcgaca aagcgatccg cttctaccgt
     3661 gcctgggttc gcttccatag taatttctgc atccgctgcc agcggcaaac gcgcacgcac
     3721 gccgtccagc agcgtttgca tcgccgggcc ggaaagcagg ctcggcgtac caccgccaat
     3781 aaagattgtc tttacttcac ggccctgagc gtaagccaca tcgttgtcca gatcgttaag
     3841 cagatgctga acataatcgt cgtgcggcac ttctcctttc aacgcgtgag agttgaaatc
     3901 gcagtacggg catttctgca cgcaccacgg gatgtgaatg tagagactca gcggcggtaa
     3961 tttaaccatt acgtaaagcg tccagcagca gtttcaatgc ctgaccacgg tgggaaatgg
     4021 cgctcttttc ttcgcgggtc agttcggcgg cggttttccc ttcggaaggt acgaagaaga
     4081 ttggatcata accaaagcca ccagtgcccg ctggttcacg agtaatcacg cccggccagc
     4141 taccgtggca taccagcgga gtcggatctt ccgcgtgacg cagatatacc agcacgcagt
     4201 ggaaacgcgc ctgacgttgg tcatccggta cgtctttcag tgtttccagc agtttttgca
     4261 gattctttag atcggtcgcg tcttcaccgg aataacgcgc ggagtaaatg cccggcgcgc
     4321 cgccaagcac atctaccgcc agaccagagt cgtcggcaat tgccggtaaa ccggtcactt
     4381 ttgccgcatg gcgcgctttc agaatcgcgt tttcgataaa ggtcaggccg gtttcttcag
     4441 cggaatctac gccgaggtct gtttgggcca cgatatcaag accgaagtcg ctaagcagcg
     4501 acgccagctc acgcacttta ccggcattgc cggttgcgag gacaactttt tgcataggat
     4561 acctaattaa ttaacgccgc gatttctggc gggatttgtt gcggattaat gattttaatt
     4621 tgtttgtggc ggccaagttc gcctttttca atcaccacct ggcttttggc aacccggaat
     4681 tgcttaccga gaaacttcac cagatgactg ttggcctgac cgtcaaccgg cggcgcggta
     4741 atggcgactt taacttcgtc gccatgtaaa ccgacaatag aatcacggct ggctttcggc
     4801 tgaatataga gccgtaaaac cagaccgtca tcattaactg ttacggcact cataacgcca
     4861 tccacagccc cggcagcagc atgtttccgg ttgcctgtaa tacttctgcg acacccatat
     4921 tgatgacata cagcagcaga acgaggatca tcggcgagaa atcaatccca cccattgccg
     4981 gtagcaggcg gcgaatcggg cgcagcagcg gatcggccag ctgaatcagc acgtattcaa
     5041 tcgggctacg accctggctt acccagctca taatcgccat caccagcagg acccagaaaa
     5101 tcagcaggcc gatggttttc agcagaatca gtaaaccggc aatccagatg attggcagga
     5161 aggtcaccac tttaaacagc acgatggctt tgataaaact gagaatatag gcaaccagca
     5221 gcgaggcgct gtcaatgggg cccattgccg gaataacgcg gcgcagtggc ccgataattg
     5281 gctgcgttac cttcactaca aactgtgaga aggggttgta aaaatcacaa tgagcccact
     5341 gcatccagat gcgtaataac agcaccatgg tatacagctc aatgaccgtt gaaagcagga
     5401 aagtcaacgt attcatggcg ttcctttaat tccttatttt ttagagtaat cacgcgcacc
     5461 aaaaattgca gtgccgatac gaaccatcgt gctacctgcc gcaatggcgg cttccatatc
     5521 gtccgacatt cccagagaga gcgtgtcgat atgcgggtag cgcgttttca gtccggcaaa
     5581 tgctacagcc atttggcgtg caacttcaaa ctgccttaca tattctgact caggtgcagg
     5641 gattgccatc agcccgcgca gacgtaaacg cggtagttca gcgaccgcag ctgccagctc
     5701 gtccagttct gccagttgaa tcccggactt actgttttca tcactaatat taatttgaat
     5761 cagtacgtta agagggggaa gttctgccgg gcgctgatcg ttgaggcggg tagcgatgcg
     5821 caaacggtcg atggtatgac accagtcgaa atgctctgcc accaggcggc ttttattaga
     5881 ctgcaacggg ccaataaaat gccattctaa tcctgttacg cccagttcct gaaagtgtcg
     5941 aattttatc
//
LOCUS       DAFATA010000178         5423 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFATA010000178 DAFATA010000000
VERSION     DAFATA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.180"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1429
                     /locus_tag="LGJ20_004581"
     CDS             <1..1429
                     /locus_tag="LGJ20_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBK2388565.1"
                     /translation="SATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGSVKVTADEVNKIMQAANISQPDADELLAVSRGKFVEGISDPNDPKWIKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADS"
     gene            1502..1753
                     /locus_tag="LGJ20_004582"
     CDS             1502..1753
                     /locus_tag="LGJ20_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBK2388566.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            1788..3068
                     /locus_tag="LGJ20_004583"
     CDS             1788..3068
                     /locus_tag="LGJ20_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBK2388567.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKHKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(3070..3198)
                     /locus_tag="LGJ20_004584"
     CDS             complement(3070..3198)
                     /locus_tag="LGJ20_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBK2388568.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHTCQLFDKY"
     gene            complement(3256..4275)
                     /locus_tag="LGJ20_004585"
     CDS             complement(3256..4275)
                     /locus_tag="LGJ20_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HBK2388569.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(4287..>5423)
                     /gene="rspA"
                     /locus_tag="LGJ20_004586"
     CDS             complement(4287..>5423)
                     /gene="rspA"
                     /locus_tag="LGJ20_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBK2388570.1"
                     /translation="GITGLGDATLNGRELSVASYLQDHLCPQLIGRDAHRIEDIWQFF
                     YKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSI
                     DEALDDYARHQELGFKAIRVQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEEQLWSTE
                     KYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDPTPAE
                     NQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIAD
                     FASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFD
                     NGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNW"
BASE COUNT         1603 a         1268 c         1259 g         1293 t
ORIGIN      
        1 gagcgcaact gatactctct cgtcgccagt accagaaact gatgcagtgg aaacggcaat
       61 tccagacaac gaaaaaaccg aatgcaaagt ggaagtcgaa ccatctgtag aacgtgaggg
      121 gccgttctac ttcctcttca ccgacaagga tggcgaaaaa tacggtcgcg caaataaact
      181 ttctggtctg gataaggcgc tgtctgctgg ggctactgaa atcacgaaag aagaatattt
      241 cgcccgcaaa aacggtacat actcaggttc acaacaaaat actggtgcat ctgacacgac
      301 cgcacaacca gggtcagtaa aagttaccgc tgacgaagta aacaaaatta tgcaggcagc
      361 caatatcagc cagcctgacg ccgatgaact gcttgcagta tcacgtggta aatttgttga
      421 agggattagc gacccgaatg atccgaaatg gattaagggg atccagaccc gcgattctgt
      481 gaaccagaac cagcaagaaa cggaacagaa cgaccagaaa gcggaacaaa acagcccaaa
      541 tacgcaacaa aacgagccag aaacgaaaca acctgaacca gtagtgcaac aggaaccgga
      601 aaagatctgc accgcctgcg gtcagagcgg tggcggcaac tgccctgatt gtggtgcggt
      661 gatgggtgac gcaacatacc aggaaatatt cgatggagag aatcagcctg aagttcagga
      721 aaatgatccg gaggaaatgg aaggtactgc gcatcagcac aaggagaaca ctggcggcaa
      781 tcagcatcat gccagcgata gtgaaactgg cgaggcgtca gatcccttaa ttaaggcaaa
      841 cggtcatcat aatctcacat ccaccagcag agcggggatt catctgatga tcgaccttga
      901 aaccatggga aaaaatcccg atgccccgat tatctcaata ggtgcaatat ttttcgatcc
      961 gcaaaccgga gatatgggac cggaatttag taagactatc gatctggaaa ctgctggcgg
     1021 agtcattgat cgggacacca ttaaatggtg gcttaaacaa tcacgcgaag cgcaatctgc
     1081 cattatgacc gatgaaatcc cgttagatga tgcactgtta caattgcggg aatttatcga
     1141 cgaaaactcc ggtgaatttt ttgttcatgt ctggggaaat ggagccaact tcgacaacac
     1201 gattttgcgc cgttcatacg aacggcaggg gatcccctgc ccgtggcgtt actacaacga
     1261 tcgcgatgta cgcacaatcg ttgagctggg gaaagccata gacttcgatg ccagaacggc
     1321 tattccattc gaaggtgagc gccataatgc acttgatgac gcccgttacc aggcaaaata
     1381 cgtttcagct atctggcaaa aactgatccc gagtcaggct gattcttaat gttcaactgt
     1441 cgccggttgt gactggtatt ctgcaaccgg cgctcgtctg atgtaagaga taaagaaatc
     1501 gatgagcgaa gtaatcatga ttgtctctcc cggcaaatgg gtatccgaag agcagttaat
     1561 tgcgctgaaa ggaataaaaa aaggtacgtt aaaaaaggcc cgggaaaaat cgtttatgga
     1621 aggaagggaa tataagcatg tcgctcatga cggtatgcca tgggataaca gtccatgctt
     1681 ttacaacctg gaagaaattg atcgctggat tgagcgccag gcatcagcaa gaccaagacg
     1741 tcatcttact tgactaaaag ccacactaac taatgagaga agttgaaatg aaatatccga
     1801 caggcgtgga aaaccatgga gggaaattac gtatctggtt tgtttataaa ggcgtaagag
     1861 tcagggaaaa cctgggggtt cctgacacag caaaaaacag gcgcgttgca ggtgagctac
     1921 gcgcctctgt ttgttacgca ataaaaactg gtgttttcga ctatgcaaaa cagtttccct
     1981 cctcacgcaa tctggaaaaa tttggtgagg cccgacaaga tttaaccata aaagaactgg
     2041 ctgaaaaatt tctggcactg aaagaaactg aagtcgcaaa aacatcactc aacacatacc
     2101 gtgccgtcat caaaaatatc ctgagcataa tcggtgaaaa aaatcttgcc tcatcgatta
     2161 ataaagaaaa attactggag gttcgtaaag agctactgac tggataccag atccccaaaa
     2221 gtaactatat tgttacacaa ccagggagat cggctgtaac tgtaaataat tacatgacaa
     2281 atcttaacgc cgtgttccag tttggtgttg ataacggtta cctggcagat aacccgttta
     2341 aggggatctc gccattaaag gaatcaagaa ccattccgga tcctctttcg cgggaggaat
     2401 tcatccgtct tatcgatgcg tgcagaaatc agcaagccaa aaatttatgg tgtgtttctg
     2461 tttatactgg cgttcgccct ggtgagctgt gtgcacttgg atgggaggac atagatctga
     2521 aaaatggaac aatgatgatc aggagaaatt tagcaaaaga ccgtttcacg gtcccaaaaa
     2581 cacaggcggg aaccaatcgg gtcattcatc ttattaagcc agcaatcgac gctctccgga
     2641 gtcagatgac attaacgaga ctgagcaaag agcatatcat tgatgttcac ctcagagagt
     2701 atggcagaac agaaaaacat aaatgcacct ttgtttttca acctgaagtg tcagcgagag
     2761 taaaaaatta tggtgaccat tttaccgttg actcaataag gcagatgtgg gacgcagcga
     2821 taaaacgtgc cggactccgc catcgaaaat catatcagtc gagacatact tatgcctgct
     2881 ggtcgctgac agctggtgct aacccggcat ttatagcaaa ccagatgggc catgcagatg
     2941 cgcaaatggt atttcaggta tacggaaaat ggatgtctga aaacaataat gcacaggtag
     3001 ctttgttaaa tacacagtta agcgagtttg ccccaaccat gccccataac gaagcaatga
     3061 aaaattaatt taatatttat caaatagttg acacgtatga ctcttgaaat ccataaattc
     3121 aagcgcagtg cccagccatc ccgatactgc tgctttcacc aaatccttag tgcttctttc
     3181 atgtttttct attgtcataa tggttatctc taaaaaagag gtaagatgcg tactacttac
     3241 tcgccgttat tggtattatt cagaaaaagt gagtaagact ttgcagcaat gtttttgatc
     3301 ctgttcaaat aaactaatgg catcagcaac atgctggaaa tcaaacgtat gggtaattaa
     3361 tttttctggt ttaattaacc ctttacttaa ccagtcgata acaaccggaa atttatttgc
     3421 atttaagcgt gaagagaaaa tagagagttc ttttccggta attccttgct gaatcacttc
     3481 agacggttca ctggagaagc ccatcaatac aatacgtgcc gctggagaag ccagcgttac
     3541 ggcctctttc aggatagaag gatgacaagc cgcatcgata attaatgtcg gcttgatgcc
     3601 tttttcagcg aaaatctcgc caagcggtgt ctggctgtta ttaatcgccc tgtctgcccc
     3661 gctctctttc gctttttcca gtcgttcatc aatgcgatcg gcaacaatca catttttaac
     3721 gttatagacg ccttttaata cctgaacgat cgtcaggccg attggaccgg caccataaac
     3781 cagaacggta tcattttcag tcggttgacc atgtccggtt acgttagccg caatggtaaa
     3841 aggttcgatc atcaccgcat attgatcggc cactgcttca ggaattttcc acgcattttt
     3901 tgccggaacc acggcatatt cactgaaacc accgtcagcg tgcacaccta atacagcaag
     3961 tgtcgtacaa acgttcggtt tacctataga gcacggatag caatgcccac agctgaccac
     4021 cggatcgaca gcaacacgtt caccgactct ggcgctttcc acgccgtcac ccaccgcatc
     4081 aatgacgcca aagaattcat gtccaatgac gcgcggatat ttcgcaaaag gattatgccc
     4141 acgataaata tggctatcag aaccacaaat tccggcaagt ttcactttta ctcgtacttc
     4201 acccgctgac ggggtgggta tttcacgttc gacaatcgcc agttgattcg gtttttcaat
     4261 taatatgctt ttcattatct tactccttac cagttccaca gcgtgccatc ttccagacgt
     4321 gcgactggta gataagcagg ttcataggga tatttcgccg ccagcttttc atcgaattcg
     4381 atgccaagac ccggtttgtc tcccggatgc atatagccgt tatcgaaagt ccagttgtgc
     4441 gggaagactt cgagcatttg ttcggaataa cccatgtatt cctggacacc gaaattgggg
     4501 acccacagat caaagtgcag cgctgcagcc atgcagactg gtgacaaatc ggaaggaccg
     4561 tgtgagccag tacgtacctg atacagcgaa gcaaaatcgg caatccggcg cataccggta
     4621 attccgcctg catgggtcag cgtggtgcgg atataatcga tgagttgctc ttcaatcagt
     4681 tgtttgcagt cccagatgct gttgaagact tcacccactg cgatgggtgt gacggtatgt
     4741 tggcgaatga gacggaagca ttcctggttt tctgcaggcg tcgggtcttc catccagaac
     4801 atgcgataat cttcaatgct tttaccaaag cgcgccgctt caataggcgt taagcgatgg
     4861 tgcatgtcat gcagcaaatg ttcattaaaa ccaaacttgt tacgtaccgc gtcaaacaat
     4921 ttcggcatga aatcgaggta tttctccgtc gaccacagct gctcttccgg ccactgtcct
     4981 ttggttgcgg gttcataagc cagaccttta cctttcgaca tgccgtaggt ggttttcata
     5041 ccagggattc cgcactgcac gcggatggct ttgaatccca gctcctgatg acgggcataa
     5101 tcatccagag cttcatcaat actgtgaccg gtggtatggc aataaaccat caccccttca
     5161 cgagacgcgc cgccgagtaa ctggtaaagc ggcatgttgg cagctttggc tttaatatcc
     5221 cacagcgcca tatcaaccgc tgaaatggcc gacatcgtaa ccggaccgcg acgccagtaa
     5281 gcacctttat agaaaaactg ccagatatct tcgatacggt gcgcatcgcg accaataagc
     5341 tgcggacaaa ggtgatcctg caaataagag gccacggaaa gctcacgtcc attgagggtg
     5401 gcatccccaa ggcccgtaat acc
//
LOCUS       DAFATA010000179         5404 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFATA010000179 DAFATA010000000
VERSION     DAFATA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.181"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            114..506
                     /locus_tag="LGJ20_004587"
     CDS             114..506
                     /locus_tag="LGJ20_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK2388571.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            503..1030
                     /locus_tag="LGJ20_004588"
     CDS             503..1030
                     /locus_tag="LGJ20_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388572.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASALKST
                     VDTLNIISTGVQDMQQVLAQLRVENQQRNQDGEARREQLRNDIAKDECAHALPDSRFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPPSLTLPHGEISVYGAIAFWMHWKPVTL
                     INGLLNYWNSAGCND"
     gene            1049..1516
                     /locus_tag="LGJ20_004589"
     CDS             1049..1516
                     /locus_tag="LGJ20_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388573.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            1509..2144
                     /locus_tag="LGJ20_004590"
     CDS             1509..2144
                     /locus_tag="LGJ20_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBK2388574.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVL"
     gene            2156..2722
                     /locus_tag="LGJ20_004591"
     CDS             2156..2722
                     /locus_tag="LGJ20_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBK2388575.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDTNPAPGKSLKEIVISAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVKLDTPVVECTNLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            2740..3069
                     /locus_tag="LGJ20_004592"
     CDS             2740..3069
                     /locus_tag="LGJ20_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HBK2388576.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            3073..3969
                     /locus_tag="LGJ20_004593"
     CDS             3073..3969
                     /locus_tag="LGJ20_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBK2388577.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3962..4492
                     /locus_tag="LGJ20_004594"
     CDS             3962..4492
                     /locus_tag="LGJ20_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBK2388578.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            4495..>5404
                     /locus_tag="LGJ20_004595"
     CDS             4495..>5404
                     /locus_tag="LGJ20_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBK2388579.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANAKYTAQDATT
                     ARKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNS"
BASE COUNT         1336 a         1497 c         1564 g         1007 t
ORIGIN      
        1 tttcctgaag atgccgtgac gtttatcggc gcatcaatcg gttttatggg cgcagagaaa
       61 gcacgcgaca aggttattgc ggcctttaat cgcagggtga aggagaagga cgaatgagca
      121 acacgtttaa attcagcagc cggagcgaaa agaatttgca gggcgtaaat cctgatctgg
      181 tgaaagtgac ccgacgggca ctggaaatct cggaagtgga ttttggtatc accgaagggt
      241 tgcgcagccg ttaccgccag aagcaacttg tggccacagg taagagccag accatgaaca
      301 gccgccacct tacggggcat gccgtggatg ttgtggctta tatcggcagc caggtgtcat
      361 gggaatggcc gctgtacgaa aaaatcgcag cagcattcag acaggccagc cgggagctga
      421 atattccggt ggaatggggc ggcgactgga agaccctgaa agacggaccg cattttcagt
      481 tgccacacgg agcctatccg gcatgaagct ctggcccacg cttggcgtcg ctttccttct
      541 gattgccgca tggggaacat ccatgcgtct gtcgtggtcg ctgggcaggg agaacgccag
      601 aaacgaagcg caggccagcg ccctgaaaag taccgtcgac accctgaata tcatcagcac
      661 cggggtacag gatatgcagc aggtgctggc gcaactccgc gtggaaaatc aacagcgaaa
      721 tcaggacgga gaggccagac gtgaacagct acgcaacgat attgcaaaag atgaatgcgc
      781 ccacgctttg cctgactctc gttttactga caggctgcgc aggcacgcag aacgcgccac
      841 ggccagcgcc gtcagtccgg cttataccgc agacgctgac cataccggta acgcctcccc
      901 cccttccctg accctcccac atggggaaat ctcggtatat ggggcgatcg ccttctggat
      961 gcactggaaa cctgtaacgc tgataaacgg gctattgaat tactggaaca gcgcaggctg
     1021 caacgactga acaacgagga taacaaccat gctgaaaact gattccctgc gtgaagccat
     1081 gacccgttca tgccgatggt gtcaggccaa cccggaaaaa ttcaccattt tcgtggagag
     1141 cggcaacatt gaaacgaccg gagaaacgcc ctcgtttgtt taccgctatc agatggtgat
     1201 gtttgtcatg gattacgccg gggagctgga cgacctcacg ctgccgctgc tggcgtggtt
     1261 atccgaaaat cagccacagt tgttgctcaa tccggagcgt aatcaggaca tcaaattctc
     1321 cgccgttatc aatgacgatg acagcgccga tctcttgttt acgctccctc tgcgggaacg
     1381 cgttcgcatc acgcgcagca gtcaggggac accgcaggca gaacacctgc cggagccaaa
     1441 accccgtctg ccctcttccg aaggcgactg gtcgcatgta ttccaggatg tgacgtgggg
     1501 tgaaagcgat ggataaggca ttcacccgcg tggatgaaac ctttgaggct atccgcgaca
     1561 gcctgaatca gcaggccatc aataacatcg ccagaaagct ggcacaggat ttacgtcgcg
     1621 cccagcaggc acgtatccgg tcacagaaag cgccggacgg gactgcatgg acaccacgca
     1681 gacgccgcgt aacccggata caggagcgca ttcgctttat ctggaataac gaagcacgca
     1741 cgctgaaaaa ctggcatcac gacacgggaa aatacgggcg aaccatcacc gggtgggatg
     1801 aggataaaaa cagcatccgc acgttttacc gggatgacat cgaccgcttt ctggaaatac
     1861 gcacccggcg catcaaccag gacagcacaa agcgcgtccc catgttcgta aaactgcgca
     1921 ccgcccgcta cctgaaagcc cgtgcagatg cttccggtgt gacggtgggt tacagcggcg
     1981 tggccgcacg tattgcacgc gttcatcagt tcggtgagcg cgatcaggtt gcgccgggca
     2041 ttttcaccga ttacccggta cgtgagctgt tgggcatcag tcaggcagat gaacgcctga
     2101 tttataacac ggtgctgggc cggattgcgg aggctgtact gtgagcgcag aactcatgcg
     2161 actgctgagc aatatcatcc gtaccgggat catctctgaa gttgatgaga agtcctggcg
     2221 cgtgcgcgtt cgcagcggcg aactggaaac aggctggctg cgctggaaca ccacgcgcgc
     2281 gggggccttc aatgtgtggc tgccgccatc accaggcgaa caggtggtaa ttgcctgcat
     2341 tggcggcaac ccggaaaccg ccatgataat tggcagcctg tggagtgata ccaatccggc
     2401 acccggcaaa agcctgaaag aaatcgtgat cagcgcgccg gacggcgcgg tgttccgcta
     2461 cgacgcggac gcaggcgcac tgagcgccag cggcatgaaa acggccactt tacaggcatc
     2521 cgtcagcgtg aaactggaca cgcccgtcgt ggaatgcaca aaccttctga gaacggcgac
     2581 gcttgacgtc acaaaagggg gaaagatgag cggcaatatc acgcacagcg gcggcaactt
     2641 cacctcaaac ggcattaccg tgcatacgca taaacacggt ggcgtgaaag gcggcagcga
     2701 ttcgacagga ggcccgcagt gacaacccgc tacacaggaa tgaatccgga cgggacggga
     2761 aacctgaacg atatggagca cctgaaacag tcagtcaggg atatcctgac caccccgctg
     2821 gcaagccggg ttatgcgacg ggaatatggc agccttgtgc ctgatttgat tgacgaaccc
     2881 atgaataaca ccacgcgtct gcaatgcatg agtgctgccg tgattgcgct gacacgatgg
     2941 gaaccccgca ttgccctgga tgccatcgac gttgtctgga aagcgggagg ccgcgccggg
     3001 gtgacgctgt cgggcactgt catgcagacc atgcagaatg ttgaattaac catcacgctg
     3061 agggagtaaa tcatgcccgc cgttgacctt tcacagttac cggaacccgc catcatcgcg
     3121 gagcctgact ttgaggcaat tctggctgac acaaaggcca tgatgattgc ggcttatccc
     3181 gccgaacagc gtgaagccgt ctccgccgcg ctggagctgg aatcggaacc cctgaacgtt
     3241 atcgcccaga caacagcgtt tcgtgaaatg ctgttacgcc agcgggtcaa tgagggggca
     3301 cgcgcctgca tgttaagcca cagcgccggg acagacctgg acaacctcgc gggcaatatg
     3361 aacacaaagc gcctgaccat cactccggca acggatacca ccgacgcagt gatggaaagt
     3421 gacacctcgc tgagactgcg ggcacagcgg gcgtacgatg gcctgagtgt tgctggcccg
     3481 tcaggtgcat acgagtattt tgcccgcagc gccagcggtc tggtgcgtga tgcgcgggct
     3541 atcagtccgt ctccggcaaa tgtgacggtt tccatcctgt ccactgaagg cgacggcaca
     3601 gcaacggagg cgttgcttaa taccgttcgc gccgttctga atgcagagga tacccgcccg
     3661 gtggccgacc gccttactgt acagagcgcc agaatcgtga catggcggct gaatgcaaaa
     3721 ctgtactttt accccggccc ggaatccgaa cctattctgg cggcggctga atcgtcgttc
     3781 aggaagtggc tggctgagca ggggcttatc ggtcaggacg tggcgttgtc cgccattgct
     3841 gccgcactgc atgtgcacgg tgtgcaacgc gtggagataa tcgaacccac acagaatatg
     3901 gccatcagcg acatacaggc ggcgcgctgt gagtcgttca ccatcagcga aggtgggcgc
     3961 aatgagtaat tcactgttac caccatcagc cagcagtttc atgcgttgtg ccgaagctgt
     4021 cggaacgcgc attacagaca tcccggtaga cctcaacacg ctgtggtcgc cggacacctg
     4081 cccggtgcac ctgctgcctt atctcgcctg ggcattttcc gttgaccgtt gggatcgcaa
     4141 ctggccggaa gagacaaaac gacaggtgat tcgtgatgca tggctgatac accgacacaa
     4201 agggaccatc agcgcactgc gcagggccat tgagccgctg ggatacctca ttcgcgtgtc
     4261 tgagtggtgg gagttcggcg gagaaccggg aacatttacc gttgaagtcg gcacactgga
     4321 cagtggcgtg acggaggaaa tgtatctgga aatggagcgg ttgattgctg atgcccgtcc
     4381 ggtcagccgc cacatgacag ggctgaatat cattcaggaa attccggggg atatttttgc
     4441 agcggcggca acttatgacg gtgaagttat taccatttat ccggacgatt aagcatgagt
     4501 accacaacac gaaaatttaa aaccgttatc accgatacag gtgccaaaaa attagctcag
     4561 gcagccgctc cagatggtaa gcctgtccgc ctgactcata tggccgtggg cgacggtggc
     4621 ggcacgttgc ccacaccaga cagtaagcag acccgtctgg tgcatgaggt gtggcgacac
     4681 actgttaatc gcgtcatcct ggacgcaaca catcagaacc gcattattgc ggagctggtt
     4741 attcctcctg aaacgggcgg attctggatc cgggaaattg gtgtatttga tgagcacggc
     4801 gatttaatcg cggtgggcaa tactgccgaa agttacaaac caaccgttgc cgaagggtcc
     4861 ggacgtgcac aaacatttcg caccattctg accgtatcca gcacggccac cgtggcgctt
     4921 accgtggata acaccatggt gatggccaca gtggattacg tggatgacaa actgaaagag
     4981 catgaacagt cacgacgtca cccggacgcc tcgctgaccg caaaaggctt tgttcaactc
     5041 agtagcgcca ctaacagcgt gtctgaaacg caggctgcaa cgccgaaagc ggttaaggcc
     5101 gcgtatgacc ttgctaacgc aaaatatacc gctcaggacg ccacgacggc acgaaaaggc
     5161 cttgtccagc tcagtagcgc caccaacagc acgtctgaaa cgcaggctgc aacgccgaaa
     5221 gcagtaaagg ccgcgtatga ccttgctaac gcaaaatata ccgctcagga cgccacgacg
     5281 gcacaaaaag ggatagtcca gctcagtagt gccaccaaca gcacgtctga aacactggcc
     5341 gcgacatcga aagcggttaa ggcggtaatg gatgaaacga acaagaaagc gcccttaaac
     5401 agtc
//
LOCUS       DAFATA010000180         5395 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFATA010000180 DAFATA010000000
VERSION     DAFATA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.182"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            114..506
                     /locus_tag="LGJ20_004596"
     CDS             114..506
                     /locus_tag="LGJ20_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK2388580.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            503..910
                     /locus_tag="LGJ20_004597"
     CDS             503..910
                     /locus_tag="LGJ20_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HBK2388581.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKRT
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            1048..1515
                     /locus_tag="LGJ20_004598"
     CDS             1048..1515
                     /locus_tag="LGJ20_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388582.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            1508..2143
                     /locus_tag="LGJ20_004599"
     CDS             1508..2143
                     /locus_tag="LGJ20_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBK2388583.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVPRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDKDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            2155..2721
                     /locus_tag="LGJ20_004600"
     CDS             2155..2721
                     /locus_tag="LGJ20_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBK2388584.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVISAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTNLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            2739..3068
                     /locus_tag="LGJ20_004601"
     CDS             2739..3068
                     /locus_tag="LGJ20_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HBK2388585.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILITPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            3072..3968
                     /locus_tag="LGJ20_004602"
     CDS             3072..3968
                     /locus_tag="LGJ20_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBK2388586.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPTEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSTGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSASIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3961..4491
                     /locus_tag="LGJ20_004603"
     CDS             3961..4491
                     /locus_tag="LGJ20_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBK2388587.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLRSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLTHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            4494..>5395
                     /locus_tag="LGJ20_004604"
     CDS             4494..>5395
                     /locus_tag="LGJ20_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBK2388588.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAP"
BASE COUNT         1330 a         1505 c         1564 g          996 t
ORIGIN      
        1 tttcctgaag atgccgtgac gtttatcggc gcatcaatcg gttttatggg cgcagagaaa
       61 gcacgcgaca aggttattgc ggcctttaat cgcagggtga aggagaagga cgaatgagca
      121 acacatttaa attcagcagc cggagcgaaa agaatttgca gggcgtaaat cctgatctgg
      181 tgaaagtgac ccgacgggca ctggaaattt cggaagtgga ttttggtatc accgaagggt
      241 tgcgcagccg ttaccgccag aagcaacttg tggccacagg taagagccag accatgaaca
      301 gccgccacct tacgggacat gccgtggatg ttgtggctta tatcggcagc caggtgtcat
      361 gggaatggcc gctgtacgaa aaaatcgcag cagcattcag acaggccagc cgggagctga
      421 atattccggt ggaatggggc ggcgactgga agaccctgaa agacggaccg cattttcagt
      481 taccacacgg agcctatccg gcatgaagct ctggcccacg cttggcgtcg ctttccttct
      541 gattgccgca tggggaacat ccatgcgtct gtcatggtcg ctgggccggg agaacgccag
      601 aaacgaagcg caggccagca ccctgaaacg taccgccgac accctgaata tcatcagcgc
      661 cggggtacag gatatgcagc aggtgctggc gcaactccgc gtggaaaatc agcaacgcaa
      721 tcaggacgga gaggcaagac gtgaacagct acgcaacgat attgcaaaag atgaatgcgc
      781 ccacgctttg cctgacgctc gttttactga caggctgcgc aggcacgcag aacgcgccac
      841 ggccagcgcc gtcagtccgg cttataccgc agacgctgac cataccggta acgcctcccc
      901 ccttccctga cactcccaca tggggaaatc tcggtatatg gggcgaccgc cttctggatg
      961 cactggaaac ctgtaacgcg gataaacggg ccattgaatt actggaacag cgcaggctgc
     1021 aacgactgaa caacgaggac aacaaccatg ctgaaaactg attccctgcg tgaagccatg
     1081 acccgttcat gccgatggtg tcaggccaac ccggaaaaat tcaccatttt cgtggagagc
     1141 ggcaacattg aaacgaccgg agaaacgccc tcgtttgttt accgctatca gatggtgatg
     1201 tttgtcatgg attacgccgg ggagctggac gacctcacgc tgccgctgct ggcgtggtta
     1261 tccgaaaatc agccacagtt gttgctcaac cctgagcgta atcaggacat caaattctcc
     1321 gccgttatca atgacgatga cagcgccgat ctcctgttta cgctccccct gcgggaacgc
     1381 gttcgcatca cgcgcagcag tcagggcaca ccgcaggcag aacacctgcc ggagccaaaa
     1441 ccccgcctgc catcttccga aggcgactgg tcgcatgtat tccaggatgt gacgtggggt
     1501 gaaagcgatg gataaggcat tcacacgcgt ggatgaaacc tttgaggcca tccgcgacag
     1561 cctgaatcag caggccatca ataacatcgc cagaaagctg gcacaggatt tacgccgcgc
     1621 ccagcaggcg cgtatccggt cacagaaagc gccggacggg accgcgtgga caccacgcag
     1681 acgccgcgta ccccggatac aggaacgcat tcgctttatc tggaataacg aagcacgcac
     1741 gctgaaaaac tggcatcacg acacggggaa atacgggcga accattaccg ggtgggataa
     1801 ggataaaaac aatatccgca cgttttaccg ggatgacatc gaccgttttc tggaaatacg
     1861 cacccggcgc atcaaccagg acagcacaaa gcgcgtcccc atgttcgtaa aactgcgcac
     1921 cgcccgctac ctgaaagcac gtgcagatgc ttccggtgtg acggtgggtt acagcggcgt
     1981 ggccgcacgt attgcccgcg ttcatcagtt cggtgagcgc gatcaggttg cgccgggcat
     2041 tttcaccgat tacccggtac gtgagctgct gggtatcagc caggcagatg agcgcctgat
     2101 ttataacacg gtgctgggcc ggattgcgga ggctgtacgg tgagcgcaga actcatgcga
     2161 ctgctgagca atatcatccg caccgggatc atctctgaag ttgatgagaa gtcctggcgc
     2221 gtgcgcgttc gcagcggcga actggaaaca ggctggctgc gctggaacac cacgcgcgcg
     2281 ggagccttca atgtgtggct gccgccatca ccaggcgaac aggtggtaat tgcctgcatt
     2341 ggcggcaacc cggaaaccgc catgataatt ggcagcctgt ggagtgatgc cagtccggca
     2401 cccggcaaaa gcctgaaaga aatcgtgatc agcgcgccgg acggcgcggt gttccgctac
     2461 gacgcggacg ctggcgcact gagcgccagc ggcatgaaaa cggccacttt acaggcatcc
     2521 gtcagcgtga cactggacac gcccgtcgtg gaatgcacaa accttctgag aacggcgacg
     2581 cttgacgtca caaaaggagg aaagatgagc ggcaatatca cgcacagcgg cggcaacttc
     2641 acctcaaacg gcattaccgt gcatacgcat aaacacggtg gcgtgaaagg cggcagcgat
     2701 tcgacaggag gcccgcagtg acaacccgct acacaggaat gaatccggac gggacgggaa
     2761 acctgaacga tatggagcac ctgaaacagt cagtcaggga catcctgatc accccgctgg
     2821 caagccgggt tatgcgacgg gaatatggca gccttgtgcc tgatttgatt gacgaaccca
     2881 tgaataacac cacgcgtctg caatgcatga gtgctgccgt gattgcactg acacgatggg
     2941 aaccccgcat tgccctggac gccatcgacg ttgtctggaa ggcaggaggc cgcgccgggg
     3001 tgacgctgtc gggcactgtc atgcagacca tgcagaatgt tgaattaacc atcacgctga
     3061 gggagtaaat catgcctgcc gttgaccttt cccagttacc ggaacccgcc atcatcgcgg
     3121 agcctgactt tgaggcaatt ctggctgaca caaaggccat gatgattgcg gcttatccca
     3181 ccgaacagcg tgaagccgtc tccgccgcgc tggaactgga atcggaaccc ctgaacgtta
     3241 tcgcccagac aacagcgttt cgtgaaatgc tgttacgcca gcgggtcaat gagggtgcac
     3301 gcgcctgcat gttaagccac agcaccggga cagacctgga caacctcgcg ggcaatatga
     3361 acacaaagcg cctggttatc actccggcaa cggataccac cgacgcggtg atggagagcg
     3421 acacctcgct gagactgcgg gcgcagcggg cgtacgacgg cctgagtgtt gctggcccgt
     3481 caggtgcata cgagtatttt gcccgcagcg ccagcggtct ggtgcgtgat gcgcgggcta
     3541 tcagtccgtc tccggcaaat gtgacggttt ccatcctgtc cacagagggc gacggcacag
     3601 caacggaggc gttgcttaat accgttcgcg ccgttctgaa tgcagaggat acccgcccgg
     3661 tggccgaccg cctgaccgta cagagcgcca gcatcgtgac atggcggctg aatgcaaaac
     3721 tgtactttta ccccggcccg gaatccgaac ctattctggc ggcggctgaa tcgtcgttca
     3781 ggaagtggct ggctgaacag gggcttatcg gtcaggacgt ggcgttgtcc gccattgctg
     3841 ccgcactgca tgtgcacggt gtgcaacgcg tggagataat cgaacccaca cagaatatgg
     3901 ccatcagcga catacaggcg gcgcgctgtg agtcattcac catcagcgaa ggtggacgca
     3961 atgagtaatt cgttgttacc accatcagcc agcaatttca tgcgttgtgc cgaagccgtc
     4021 ggaacacgca ttacagacat tccggtagac ctcaacacgc tgcggtcgcc ggacacctgc
     4081 ccggtgcatc tgctgcctta tctcgcctgg gcgttttccg ttgaccgctg ggatcgcaac
     4141 tggccggaag agacaaagcg acaggttatt cgtgatgcat ggctgacaca ccgacacaaa
     4201 gggaccatca gcgcactgcg ccgggccatt gagccgctgg gatacctcat tcgcgtgtct
     4261 gagtggtggg agttcggcgg agaaccggga acatttaccg ttgaagtcgg cacgctggac
     4321 agtggcgtga cggaggaaat gtatctggaa atggagcggt tgattgctga tgcccgcccg
     4381 gtcagccgcc acatgacagg gctgaatatc attcaggaaa ttccggggga tattttcgca
     4441 gcggcggcaa cttatgacgg tgaagttatt accatttatc caggcgatta agcatgagta
     4501 ccacaacacg aaaatttaaa accgttatca ccgatacggg tgcaaaaaaa ttagctcagg
     4561 cagccgcgcc agatggtaag cctgtccgcc tgactcatat ggccgtgggc gacggtggcg
     4621 gcacgttgcc cacaccagac agtaagcaga cccgtctggt gcatgaggtg tggcgacata
     4681 ctgttaatcg cgtcatcctg gacgcaacac atcagaaccg cattattgcg gagctggtta
     4741 ttcctcctga aacgggcgga ttctggatcc gggaaattgg tgtatttgat gagcacggcg
     4801 atttaatcgc ggtgggaaat actgccgaaa gttacaagcc agccgttgcc gaagggtccg
     4861 gtcgtgcaca aacatttcgc accattctga ccgtatccag cactgccacc gtggcgctta
     4921 ccgtggataa caccatggtg atggccacag tggattacgt ggatgacaaa ctgaaagagc
     4981 atgaacagtc acgacgtcac ccggacgcct cgctgaccgc aaaaggcttt gttcaactca
     5041 gtagcgccac taacagcgtg tctgaaacgc aggctgcaac gccgaaagca gtaaaggccg
     5101 cgtatgatct tgctaacggt aaatatactg cgcaggatgc cagcacgacg cgaaaaggcc
     5161 ttgtccagct cagtagcgcc accaacagca cgtctgaaac gcaggctgca acgccgaaag
     5221 cagtaaaggc cgcgtatgac cttgctaacg caaaatatac cgctcaggac gccacgacgg
     5281 cacaaaaagg gatagtccag ctcagtagtg ccaccaacag cacgtctgaa acactggccg
     5341 cgacatcgaa agcggttaag gcggtaatgg atgaaacgaa caagaaagcg ccctt
//
LOCUS       DAFATA010000181         5323 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFATA010000181 DAFATA010000000
VERSION     DAFATA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.183"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..482)
                     /gene="glpC"
                     /locus_tag="LGJ20_004605"
     CDS             complement(<1..482)
                     /gene="glpC"
                     /locus_tag="LGJ20_004605"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBK2388589.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     "
     gene            complement(479..1738)
                     /gene="glpB"
                     /locus_tag="LGJ20_004606"
     CDS             complement(479..1738)
                     /gene="glpB"
                     /locus_tag="LGJ20_004606"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBK2388590.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(1728..3356)
                     /gene="glpA"
                     /locus_tag="LGJ20_004607"
     CDS             complement(1728..3356)
                     /gene="glpA"
                     /locus_tag="LGJ20_004607"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBK2388591.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            3629..4987
                     /gene="glpT"
                     /locus_tag="LGJ20_004608"
     CDS             3629..4987
                     /gene="glpT"
                     /locus_tag="LGJ20_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBK2388592.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            4992..>5323
                     /gene="glpQ"
                     /locus_tag="LGJ20_004609"
     CDS             4992..>5323
                     /gene="glpQ"
                     /locus_tag="LGJ20_004609"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="hydrolyzes deacylated phospholipids to glycerol
                     3-phosphate and the corresponding alcohols; periplasmic;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK2388593.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLD"
BASE COUNT         1163 a         1399 c         1486 g         1275 t
ORIGIN      
        1 tctttatatt gtgcttgctg agccgctatg ctgcgatacc agcgacggaa cgtgccgaag
       61 gagtatttcg gtagcgtgcg gcgatgatcg atttttaacg ccgcatcaag cagctgccgc
      121 accggtttca gcgaggtagc ggtgttgacg attggtgcga acggcgtgga aacgctaccc
      181 atcaggtcgg tatgactcaa cacaaaatta cgcagcgacg ggcgcgtggt gtcatatttt
      241 gcccgcgcgc gctggataat atcgccaatc ttcacatcgg acgggcaggc gacttcacaa
      301 cgtttgcagt tgatgcaata tttcagcgcc tcgtcataca gtgcgccatc tttcaaacgc
      361 agacgctcgc catccggccc ggcttgtttt ggccctggat aaccgggatt cacccggctc
      421 accgggcagg cggtggtgca gacggtgcac ttaatgcagt tttcgaagct ggtgtcgttc
      481 attgttggcc tcctgcgcgt tgggcaatct gttgagcggc atgtaaagca ctgacggcac
      541 aaacaccgcc gccgcatccc tgggcgatgg gatcaaatcc gcccagcacc gaaccgatgg
      601 caaacaggtt ctcaatggtt tgccctgcct gcgacgggcg tagcgtctca tcagtggtta
      661 caccgaactg ctgccacggt tgcggcgcaa aaaaatcgcc cttataccat tcaccccgcg
      721 tggcggtttg tagcacatca aggccgagaa tcggctcgcg aatgccgtta cgttccgcta
      781 ccagtccgcc actaaagaaa ctgccgctgg cgagaaccgc gaaacgtgga cgtagcggaa
      841 tatcggcgtg attgcgggtc cagatttcat tcactacgcc atttttacag gtcacttttt
      901 tcacttcatc gcccggcatc cacacgccac cctggcgcac aaactggcgc tgtaactggt
      961 tttgcagacg aatgcccagc acggaaggcg gcagcgttgg caaaagcatc agtgaacaag
     1021 gtagtttttc attcaaccaa cgccacagtt tgtcatcggc caggccgaag caggcgggca
     1081 tcaggatcat ttcgcaggta ttggcgacag gaataagcgc atcaagtaac agcggccagt
     1141 tttcttcatt atcaaggaaa cgggcgatat tcacagcgcg aaattcggtg gcgttattgc
     1201 gcaggacatc cagttccggc agctctattt ctgcggtttc aaccgaaaga tccagttcgc
     1261 gcaacgaagc cgccgcaaga tgcgcctgga aatccatcag cccgcttatc cccactacac
     1321 atatcttctt cgcgggtagc ggccagacgg gaacttctgg cgaacttaac caggttgcac
     1381 gcaaagtgcc gagcggggta acgcgctgat gggccagttc cacgctgccc tgcaattgcg
     1441 caccgctttc agcgatcaat gcctgcgcct ggcaagcgag atcgagcacg cgttgtggtt
     1501 cgagaagact gtaaggatgg gctggtgcct gctgacgcaa agattccagt ccactgtgaa
     1561 tgtctgtcac cggttgacca tcaggcagat ggctcagcaa atccagcgaa ccggatgaga
     1621 aatgcagcgc gctttgacca cgcgtgacaa tggcacagcg caggccgtgt ttttgcagtt
     1681 gcaggccaca gagtaatccg gcgaggccgc cgcccataat gacagtatca aagcgcatct
     1741 ttctgctcct tctccagacc acacagtccc tgataaaccc agcgggtaaa ttcgctttcg
     1801 cgcagtgcgt ctccccaggc gatgggttgc acgcctttcc agcgttcgtt aaggaaggtg
     1861 gaaagttgct cgatagattg cgcggacgtc gtgacgttaa aacgttgcag cagtccggca
     1921 gcgcggcagg cgcagagttc gccctggcag gtacccatcc ccacacgggt acgacggcgt
     1981 aaatccagca ggctattaac gtttaaattt tctaccgcgt actgaacttc acccgcagtt
     2041 accgcttcgc actcacatac caggctacga tgcagacggc cttcgctcag ccaggcaggc
     2101 gtgcgatcgc catgacgata aaccgcagaa ccgcgcagcg gggcaggcag ggagatgact
     2161 ttacgcaagg taacttcagc gggttcttgt gaaccaggca gtgccagatc ggcagtcgta
     2221 caggggcgcg tgttgcccag tttgcggcat accgcgtcgg tagcccattc agccatcagc
     2281 cgataggtca tcagtttgcc accggtgatg gtgataaatc cgtccagacc atcgcgttca
     2341 gcatggtcga gcagcacgat gccacggctg acgttacgtc cgctcgggtc gtcatcgctg
     2401 gcaaccagcg ggcgcacgcc agaataggcc cgcaaaatgc gcgttttcgc catcacgggg
     2461 gccagttttt ccccttcacg cagcagaata tcaacctctt ctgccgtcac tcgattatcg
     2521 tcaatctcgt tgtaatcaat acgtaaagag gtggtaccaa tcagcgaaat ggtatcgcca
     2581 ggcaccagaa tatcggcgtc ggaaggttta cggcagcggt tgatcacatg ctggttaatg
     2641 cggtgatcca tgatcagcag cgatcctttc gccgggaaca tgcgaatgcg cagatcggca
     2701 tattcggcaa tgtgttgccc ccagatccca gcggcattaa ccacgacagg tgcatgaagg
     2761 gcctgagttt cgccggtgag atgattacgt acacgaacac cgcacaccgt cgcgccttca
     2821 cgaatcagcc ccgtgacttc atgagcggta aggataacgg caccgtgttc tttggcatcc
     2881 agcatgtttg ctgcggtcag acgaaatgga tcaacggtgc catccggaac tttcaccgcg
     2941 ccaatcagtg ccgggttaac ggcaggttcg ataatgcgcg cttgctgcgg gtctatagct
     3001 tctgcgctga tccctgcttc ttcgcaggcg cgaataaaag tggcctggaa tgagaggtca
     3061 tcttccggca gggtgataaa caggccgttg gttggttcaa cgcagtgacg tgcaatgcgt
     3121 ttcaggatct ggttttcact aatgcattcg cgggccgatt ccgcatcggt taccgcatag
     3181 cgcgcaccgc tgtgcagcag gccgtggtta cgcccggtgg caccggttgc gatgtcgtgg
     3241 cgctcaacca aaatcacgcg cagcccgcgc agggcacagt cgcgggcaat cccggctccc
     3301 gttgcgccgc caccaatgat aatcacgtca cttgattgcg agtcgcgagt tttcattgtt
     3361 atccctctga agttcgtttt ttaccattta gccatagtaa aaacatgaat tgtttgattt
     3421 cgcgcatatt cgctcataat tcgaaagtga aatgtgattt catgcgtcat tctgaacatt
     3481 ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttctga atgttttctt
     3541 aacatcgcgg caactaaaga aacggcaggt tctcccactg aatcagtctg ttaatcataa
     3601 ataagaccac gggccacgga ggctttcaat gttgagtatt tttaaaccag cgccacacaa
     3661 agcgcgctta cctgctgcgg agatcgatcc gacttatcgt cgattgcgct ggcaaatttt
     3721 cctggggata ttctttggct atgcggctta ctatttggtt cgtaagaact ttgcgcttgc
     3781 tatgccttat ctggttgagc agggattctc acgcggtgat ttaggttttg ccctttcggg
     3841 gatctcgatt gcttatggat tttcgaaatt catcatgggt tcggtatcgg atcgctcgaa
     3901 tccgcgcgtt ttcctgcccg caggtttgat tctggcggcg gcagtgatgt tgtttatggg
     3961 ctttgtgcca tgggcgacgt cgagcattgc ggtgatgttt gtactgttgt tcctctgcgg
     4021 ttggttccag gggatggggt ggccgccgtg tggtcgtact atggtgcact ggtggtcgca
     4081 gaaagaacgt ggcggcattg tgtcagtgtg gaactgtgcg cacaacgtcg gtggtggtat
     4141 tccgccgctg ctgttcctgc tggggatggc ctggttcaat gactggcatg cggcgctcta
     4201 tatgcctgct ttctgcgcca ttctggtggc attattcgcc tttgcgatga tgcgcgatac
     4261 cccgcaatcc tgtggcttgc cgccgatcga agagtacaaa aatgattatc cggacgacta
     4321 taacgaaaaa gcggaacagg agctgacggc gaagcaaatc ttcatgcagt acgtactgcc
     4381 gaacaaactg ctgtggtata tcgccatcgc caacgtgttc gtttatctgc tgcgttacgg
     4441 catcctcgac tggtcaccga cttatctgaa agaggttaag catttcgcgc tggataaatc
     4501 ctcctgggcc tacttccttt atgaatatgc aggtattccg ggcactctgc tgtgcggctg
     4561 gatgtcggat aaagtcttcc gtggcaaccg tggggcaacc ggcgttttct ttatgacact
     4621 ggtgaccatc gcgactatcg tttactggat gaacccggca ggtaacccaa ccgtcgatat
     4681 gatttgtatg attgttatcg gcttcctgat ctacggtcct gtgatgctga tcggtctgca
     4741 tgcgctggaa ctggcaccga aaaaagcggc aggtacggca gcgggcttta ccgggctgtt
     4801 tggttacctg ggcggttcgg tggcggcgag cgcgattgtt ggctacaccg tggacttctt
     4861 cggctgggat ggcggcttta tggtaatgat tggcggcagc attctggcgg ttatcttgtt
     4921 gattgttgtg atgattggcg aaaaacgtcg ccatgaacaa ttactgcaaa aacgcaacgg
     4981 aggctaatgg catgaaattg acgctgaaaa accttagcat ggcgatcatg atgagcacta
     5041 tagtcatggg aagcagtgca atggcggcgg acagcaacga aaaaatagtc atcgcccatc
     5101 gcggtgccag tggatatttg ccggagcata cgctgccagc aaaagcgatg gcgtatgcgc
     5161 agggagcgga ttatctggaa caggatttgg tgatgaccaa agacgaccat ctggttgttc
     5221 tgcatgacca ttatctcgat cgtgttactg atgttgccga tcgtttcccg gatcgggcgc
     5281 gcaaagacgg tcgttactac gcgatagatt tcacgctgga tga
//
LOCUS       DAFATA010000182         5274 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFATA010000182 DAFATA010000000
VERSION     DAFATA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.184"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1140
                     /locus_tag="LGJ20_004610"
     CDS             <1..1140
                     /locus_tag="LGJ20_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBK2388594.1"
                     /translation="VLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAG
                     WLTDGTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITA
                     EKSTTAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARV
                     LDREAKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTM
                     AAWTQDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWG
                     APRSTCQLLPKAKAWLAKKKPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRL
                     FIRNFFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            1145..1921
                     /locus_tag="LGJ20_004611"
     CDS             1145..1921
                     /locus_tag="LGJ20_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBK2388595.1"
                     /translation="MRKIFLPLLLVALSPVVHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            2196..5045
                     /gene="rcsC"
                     /locus_tag="LGJ20_004612"
     CDS             2196..5045
                     /gene="rcsC"
                     /locus_tag="LGJ20_004612"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBK2388596.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
BASE COUNT         1257 a         1260 c         1463 g         1294 t
ORIGIN      
        1 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
       61 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
      121 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
      181 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
      241 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
      301 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
      361 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
      421 gaaaagggca aactgaaatt gcagtcacat ctcgatcgtg aagagtacgt tgccagagtt
      481 ctggatcgcg aggcgaaaag tacgccccct gaggcagcga aagccatgac cgtggcgatt
      541 cgcacttttt tacagcaaaa cgccaatcgg gaaggcgatt gcctgaccat ccctgacagc
      601 agcgccacgc agcgtgtttc ggcttcgcca gctacgacgg gggcgcgaac gatggccgcc
      661 tggacgcagg atctcatcta tgcgggcgat ccggttcatt atcatggcag tcgcgctacc
      721 gaagggacgc tttcctggcg gcaagccacg gcccaggcgg ggcagggaga gcgttacgac
      781 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc gccacgctca
      841 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaaagcc gcagtggcgg
      901 cgtatattac aagctgagac ggggtacaac gaaccagacg tgtttgcggt ttgccgttta
      961 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     1021 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     1081 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     1141 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgttcacag
     1201 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggtagaggg
     1261 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     1321 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     1381 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     1441 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     1501 cccggatggg caaagccgac aaaaaatgca gttttactca acgccgggta caggaacgat
     1561 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     1621 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     1681 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     1741 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     1801 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     1861 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     1921 atccatccct tcacacaacc tggctctctc ttttttcaga taagactgct gggcgatctg
     1981 ccgacgccag gatataacca ttaacaagcg tcggcagatt ggtcatttct tatttttcgt
     2041 actgagaact gtccatctac tccggctttc ctggttaagg caatggtttc taagcgatac
     2101 gcattattgc aatggtgaca gaacattgcg gtaacacgct tttaccgcta ccttaaccac
     2161 actccatcgg tcacctgagg cggagcttcg cccctttgaa ataccttgct tcttttcgta
     2221 caaccctgaa agcctcgcgc tacatgttca gagcattggc gttagtgctc tggctgttga
     2281 ttgctttttc atccgttttt tacatcgtta atgcgttaca tcagcgagaa tcggaaattc
     2341 gtcaggaatt taatctgagt tccgatcagg ctcagcgctt tattcaacgc acctctgatg
     2401 tgatgaaaga gctgaagtac atcgccgaaa atcgcttatc ggcagaaaac ggtgtgcttt
     2461 ccccgcgtgg acgagaaacg caggcggatg tgcctgcgtt tgaaccgctg tttgccgact
     2521 ccgattgttc cgcaatgagt aacacctggc gaggttctct ggagtcattg gcgtggttta
     2581 tgcgctactg gcgcgataat ttttctgcgg cttacgatct caaccgggta tttttaatcg
     2641 gcagtgataa cctctgcatg gccaatttcg gtctgcgtga tatgccagtg gaacgcgata
     2701 ccgcgttgaa agctttgcat gaacgcatca ataaatatcg aaatgcacca caagatgata
     2761 gcggcagtaa cctctactgg atcagcgaag gtccgcgccc tggcgtcggg tatttttacg
     2821 cgttgacgcc agtttatctg gcgaaccggt tgcaggcgct tttgggtgtc gagcagacca
     2881 tccggatgga gaactttttc ttaccgggta cgttgccgat gggggttacc attcttgatg
     2941 aaaatggtca taccctgatt tcgcttaccg gaccagaaag taaaattaag ggcgatcctc
     3001 gctggatgca ggaacgctcc tggtttggct atacggaagg gttccgggag ctggtgctga
     3061 agaaaaatct gccaccctca tcgctaagca tcgtgtattc ggtgccggtt gataaggtgc
     3121 tggaacgcat tcgcatgttg atccttaacg caattttgct gaatgtgctt gccggagctg
     3181 cattgtttac tctcgcgcgg atgtacgagc gacgtatttt cattccggcg gaaagcgacg
     3241 ccctgcgact ggaagaacat gagcagttca atcgcaagat tgtcgcctcc gcgccagtgg
     3301 gtatctgcat tttgcgtacc gctgatggcg tcaatatttt aagtaacgaa ctggcgcata
     3361 cctatctcaa tatgcttacg catgaggacc gccaacgact gacgcaaatt atctgtgggc
     3421 agcaggtcaa ttttgttgat gtcctgacca gcaacaatac caatctgcaa atcagcttcg
     3481 tccattcgcg ctatcgtaat gaaaacgtgg ccatttgtgt gctggtggat gtttcttcgc
     3541 gcgtgaagat ggaagagtcg ttgcaggaga tggcacaagc agcggaacag gcgagccagt
     3601 caaaatcgat gttccttgcc accgtcagtc atgagctgcg aacgccgctg tatggcatta
     3661 tcggtaacct ggatctgttg caaaccaaag agttaccgaa aggcgtcgat cggctggtga
     3721 cggcaatgaa caactcttcc agcctgttgt tgaaaattat cagcgatatt ctcgatttct
     3781 cgaagattga atcggaacag ttgaagatcg aaccgcgtga gttttcaccg cgtgaagtga
     3841 tgaaccacat caccgccaac tatttaccgc tggtggtacg caagcagtta ggcttgtact
     3901 gctttattga accggatgtg ccagtggcct taaatggcga cccgatgcgt ttacagcagg
     3961 tcatctccaa cctgttgagt aacgccataa aattcaccga taccggctgt atagttttgc
     4021 atgtccgcgc tgatggcgat tatctctcta tccgtgttcg cgataccggc gtggggattc
     4081 cggcgaaaga agtggtgcgc ttgtttgatc ccttcttcca ggtcggaacg ggcgtacagc
     4141 gaaacttcca ggggaccggt ctgggtctgg cgatttgtga aaaactgatc agcatgatgg
     4201 acggcgatat ctcggtagat tcagaaccgg gaatgggcag ccagtttacc gtgcgtattc
     4261 cgttgtacgg cgctcagtac ccgcagaaaa aaggcgtgga agggttgagt ggtaaacgct
     4321 gctggctggc ggtccgcaat gcgtcgctct gtcagttcct ggaaaccagt ttgcagcgca
     4381 gcggcatcgt cgttacaaca tacgaagggc aggaaccgac tcccgaagat gtgttaatta
     4441 ctgacgaggt agtgagtaaa aaatggcagg gtagagcggt agtgaccttc tgtcgtcgcc
     4501 atattggtat tccgctggag aaagcgccag gggagtgggt acacagtgtg gctgccccgc
     4561 atgagctacc ggcattgttg gcgcgtattt atttgatcga gatggagagc gacgatcctg
     4621 ctaacgctct gccgtcgacg gacaaagcgg tcagcgataa tgacgatatg atgattctgg
     4681 tcgtggatga tcatccgatt aaccgacgtc tgctggcaga tcagttggga tcgttgggct
     4741 atcaatgtaa aaccgcgaat gatggtgtcg atgcgcttaa tgtacttagc aagaatcata
     4801 ttgatatcgt gcttagcgac gtcaacatgc caaatatgga tggttaccgc ttgacgcaac
     4861 gcattcgtca gttgggactg acgttgccgg taatcggagt aactgctaat gcgttggctg
     4921 aagagaagca gcggtgtctg gagtccggta tggacagctg cctgtcgaag ccggtaacgc
     4981 tggatgtgat aaaacagacg ctgacggtat atgccgagag ggtcaggaaa tcgcgggaat
     5041 cgtaggacgg ataaggtgtt tatgccgcat ctggcattca gtgcaaatgc cagatgcgac
     5101 gctgacgcgt cttatctggc ctactttaat gtaatctgtt gaaataatgg gaatcgtagg
     5161 ccggataagg cgtttacgct gcatccggca atcggtgcaa atgccagata agacactaac
     5221 gcgtcttatc tggcctacag atgattagtc tttatctgcc ggacttaagg tcac
//
LOCUS       DAFATA010000183         5185 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFATA010000183 DAFATA010000000
VERSION     DAFATA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.185"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..84
                     /locus_tag="LGJ20_004613"
     CDS             <1..84
                     /locus_tag="LGJ20_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
                     /protein_id="HBK2388597.1"
                     /translation="TVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            137..619
                     /locus_tag="LGJ20_004614"
     CDS             137..619
                     /locus_tag="LGJ20_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2388598.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            1165..3423
                     /gene="parC"
                     /locus_tag="LGJ20_004615"
     CDS             1165..3423
                     /gene="parC"
                     /locus_tag="LGJ20_004615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK2388599.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            3656..4393
                     /gene="plsC"
                     /locus_tag="LGJ20_004616"
                     /gene_synonym="parF"
     CDS             3656..4393
                     /gene="plsC"
                     /locus_tag="LGJ20_004616"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBK2388600.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            4468..>5185
                     /locus_tag="LGJ20_004617"
     CDS             4468..>5185
                     /locus_tag="LGJ20_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HBK2388601.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNS"
BASE COUNT         1228 a         1323 c         1440 g         1194 t
ORIGIN      
        1 acggttgaga ttcagggtga agtcgataaa gactggaatt ctgttgaaat tgacgtcaaa
       61 cagatccgca aagtaaatcc gtaattgtta ccgctcccgg gacgcgttcc cgggaataat
      121 ttcgcaggga ggcaaaatga caaacctgac actggatgta aacattatcg atttcccatc
      181 aatccctgtg gcgatgttgc cgcaccgctg tagccctgag ttgctcaact acagtgtggc
      241 gaaatttatc atgtggcgta aagagacggg gctttctcct gttaaccaaa gccagacttt
      301 tggcgtcgcc tgggacgacc ctgccaccac cgcaccggaa gcgtttcgct ttgatatctg
      361 cggcagcgtt agcgaaccga ttcccgataa tcgttatggt gtgagcaatg gtgaacttac
      421 cggtggacgt tatgccgtgg cccgccacgt tggcgagctg gacgatattt cacacacgat
      481 atggggcatt attcgccact ggctgcctgc aagcggcgag aaaatgcgta aagcaccgat
      541 tctgtttcac tacaccaatc ttgccgaagg ggtgacagag cagcgactgg aaacggatgt
      601 ttatgtgccg ttggcgtgat ggggaatctt taccggagtg tgagccaaat cactcgcgat
      661 aagtttgagc gaacaatgcc acaggcgtat tgttgttgga cttataattt ctccggtaag
      721 gaataccatg tatacgcgaa atttattatc cggtgcgccg taagccgtct cgggggtacc
      781 catgagcaag ccgcacttat ggcaggaggc gcggggcact ggtggagaca cagctaaact
      841 gggctgaacc gtatacttac agtcgactac tttgatgctt taggcttccc tggactcgca
      901 taacctcaac ttctcgaatc gcccggagcg gacccccatg ccgagtggtg tggcatgggc
      961 gcaaccttgt gctgccctct atgccgatca agtctacagc aaagagttgt atatcaaggc
     1021 acattgatgc ctttatcgcc gtcggagcac gtccggcggc gctattcacg ctgatctcct
     1081 gtgactcgac gcggcagata atgtagtatc tccggcaata ttgccccttt gaaggctggc
     1141 gaataagttg aggaatcaga attaatgagc gatatggcag agcgccttgc gctacatgaa
     1201 tttacggaaa acgcctactt aaactactcc atgtacgtga tcatggaccg tgcgttgccg
     1261 tttattggtg atggtctgaa acctgttcag cgccgcattg tgtatgcgat gtctgaactg
     1321 ggcctgaatg ccagcgccaa atttaaaaaa tcggcccgta ccgtcggtga cgtactgggt
     1381 aaataccatc cgcacggcga tagcgcctgt tatgaagcga tggtcctgat ggcgcagccg
     1441 ttctcttacc gttatccgct ggttgatggt caggggaact ggggcgcgcc ggacgatccg
     1501 aaatcgttcg cggcaatgcg ttacaccgaa tcccggttgt cgaaatattc cgagctgcta
     1561 ttgagcgagc tggggcaggg gacggctgac tgggtgccaa acttcgacgg cactttgcag
     1621 gagccgaaaa tgctgcctgc ccgtctgcca aacattttgc ttaacggcac caccggtatt
     1681 gccgtcggca tggcgaccga tattccaccg cataacctgc gtgaagtggc tcaggcggca
     1741 atcgcattaa tcgaccaacc gaaaaccacg ctcgatcagc tgctggatat cgtgcagggg
     1801 ccggattatc cgactgaagc ggaaattatc acttcgcgcg ccgagatccg taaaatctac
     1861 gagaacggac gtggttcagt gcgtatgcgc gcggtgtgga agaaagaaga tggcgcggtg
     1921 gttatcagcg cattgccgca tcaggtttca ggtgcgcgcg tactggagca aattgctgcg
     1981 caaatgcgca acaaaaagct gccgatggtt gacgatctgc gcgatgaatc tgaccacgag
     2041 aacccgaccc gtctggtgat tgtgccgcgt tccaaccgcg tggatatgga tcaggtgatg
     2101 aaccacctct tcgctaccac cgatctggaa aagagctatc gcattaacct caatatgatc
     2161 ggtctggatg gtcgtccggc ggtgaaaaac ctgctggaaa tcctctccga atggctggtg
     2221 ttccgtcgcg ataccgtgcg ccgccgactg aactatcgtc tggagaaagt cctcaagcgc
     2281 ctgcatatcc tcgaaggttt gctggtggcg tttctcaata tcgacgaagt gattgagatc
     2341 attcgtaatg aagatgaacc gaaaccggcg ctgatgtcgc ggtttggcct tacggaaacc
     2401 caggcggaag cgatcctcga actgaaactg cgtcatcttg ccaaactgga agagatgaag
     2461 attcgcggtg agcagagtga actggaaaaa gagcgcgacc agttgcaggg cattttggct
     2521 tccgagcgta aaatgaataa cctgctgaag aaagaactgc aggcagacgc gcaagcctac
     2581 ggtgacgatc gtcgttcgcc gttgcaggaa cgcgaagaag cgaaagcgat gagcgagcac
     2641 gacatgctgc cgtctgaacc tgtcaccatt gtgctgtcgc agatgggctg ggtacgcagc
     2701 gctaaaggcc atgatatcga cgcgccgggc ctgaattata aagcgggtga tagcttcaaa
     2761 gcggcggtga aaggtaagag caaccaaccg gtagtgtttg ttgattccac cggtcgtagc
     2821 tatgccatcg acccgattac gctgccgtcg gcgcgtggtc agggcgaacc actcaccggc
     2881 aaattaacgt tgccgcctgg ggcgaccgtt gaccatatgc tgatggaaag cgacgatcag
     2941 aaactgctga tggcttccga tgcgggttac ggtttcgtct gcacctttaa cgatctggtg
     3001 gcgcgtaacc gtgcaggtaa ggctttgatc accttaccgg aaaatgccca tgttatgccg
     3061 ccggtggtga ttgaagatgc ttccgatatg ctgctggcaa tcactcaggc aggccgtatg
     3121 ttgatgttcc cggtaagcga tctgccgcag ctgtcgaagg gcaaaggcaa caagattatc
     3181 aacattccat cggcagaagc cgcgcgtgga gaagatggtc tggcgcaatt gtacgttctg
     3241 ccgccgcaaa gcacgctgac cattcatgtt gggaaacgca aaattaaact gcgcccggaa
     3301 gagttacaga aagtcactgg cgaacgtgga cgccgcggta cgttgatgcg cggtttgcag
     3361 cgtatcgatc gtgttgagat cgactctcct cgccgtgcca gcagcggtga tagcgaagag
     3421 taatatgccg gggagggcct gtttcctccc gcttgcaagg aacgccggat gaaacgttaa
     3481 gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa aaattccctt gaaaccgttg
     3541 tttattcatg cgttgcgatt aacaatacgc ttttccagag agcggctttt aacaatgccc
     3601 taacctgatt tcaggtgacg tacaatgcca gtctctcaga ccttcagagg gtgttatgct
     3661 atatatcttt cgtcttatta ttaccgtgat ttacagcatc ttagtctgtg tattcggctc
     3721 catttactgc cttttcagcc cgcgtaaccc gaaacatgtg gccacctttg ggcatatgtt
     3781 tggccgtctt gcgccgctgt tcggcctgaa agttgagtgc cgcaaacctg cagatgctga
     3841 aagctacggc aatgctatct atatcgctaa ccaccagaac aactatgaca tggtgacagc
     3901 atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa aagagcttgc tgtggatccc
     3961 cttcttcggg cagttgtact ggttaaccgg caacttattg atcgacagaa acaatcgcac
     4021 taaagctcac ggcaccattg cggaagtcgt gaatcatttc aaaaaacgcc gtatttccat
     4081 ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc ctgctaccgt tcaagactgg
     4141 agcatttcac gcggcaattg cggcgggcgt cccgattatt cccgtgtgcg tctctacaac
     4201 ttcgaataag attaatctta atcgactgca caacggtctg gtgattgtcg aaatgctgcc
     4261 gccaattgac gtcagtcagt atggcaaaga tcaggttcgt gagctggctg cccattgtcg
     4321 ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa gtcgcagaac gcgaagccgc
     4381 cggaaaagtt taagtcggca atctgtattt ttgcggggaa cactttcctg cacggtatta
     4441 ctttagccag ttttacatgg agcaaatatg tcactcagtc ggcgtcagtt cattcaggca
     4501 tcggggattg cactttgtgc aggcgctgtt cccctgaagg ccagcgcagc cgggcaacag
     4561 caaccgctac ccgttccgcc gctacttgaa tctcgccgtg ggcaaccgct gtttatgact
     4621 gtacaacgtg cgcactggtc atttacgcca gggacacgcg cgtcggtctg gggaatcaat
     4681 ggtcgttacc tggggccgac tatccgcgtc tggaagggcg acgatgttaa gcttatttac
     4741 agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg ggctacaggt accaggcccg
     4801 ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg actgggcacc cgtactgccc
     4861 attcgccaga acgcagctac tctgtggtat cacgccaata ctcccaaccg cacggctcag
     4921 caggtctata acggccttgc cggaatgtgg ctggtggaag atgaagtcag caagtcgctg
     4981 cctatcccca accattatgg tgtggatgat tttccggtca ttatccagga taaacggctg
     5041 gataactttg gtacgccaga atacaacgaa ccgggaagcg gcggctttgt tggtgatacg
     5101 ctgctggtta acggtgtaca aagcccgtac gttgaagtct cgcgtggctg ggtgcgcttg
     5161 cgactgctga acgcgtcgaa ctctc
//
LOCUS       DAFATA010000184         5152 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFATA010000184 DAFATA010000000
VERSION     DAFATA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.186"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1046)
                     /locus_tag="LGJ20_004618"
     CDS             complement(<1..1046)
                     /locus_tag="LGJ20_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HBK2388602.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIIS"
     gene            complement(1224..2135)
                     /gene="gsiD"
                     /locus_tag="LGJ20_004619"
     CDS             complement(1224..2135)
                     /gene="gsiD"
                     /locus_tag="LGJ20_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBK2388603.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(2138..3058)
                     /gene="gsiC"
                     /locus_tag="LGJ20_004620"
     CDS             complement(2138..3058)
                     /gene="gsiC"
                     /locus_tag="LGJ20_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBK2388604.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(3076..4614)
                     /gene="gsiB"
                     /locus_tag="LGJ20_004621"
     CDS             complement(3076..4614)
                     /gene="gsiB"
                     /locus_tag="LGJ20_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBK2388605.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4634..>5152)
                     /locus_tag="LGJ20_004622"
     CDS             complement(4634..>5152)
                     /locus_tag="LGJ20_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK2388606.1"
                     /translation="LLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALD
                     VSIRGQIINLLLDLQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFE
                     NPQHPYTRKLLAAVPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYV
                     AQPQSEYAFMRR"
BASE COUNT         1378 a         1396 c         1172 g         1206 t
ORIGIN      
        1 atgctgataa tctctttcgc atcgccttcg cgaattttca gagcctcgat ctgatccgtt
       61 gagtcgggca aaaacgccag cgtattaacc agctcggtca tttcagaaaa ttgccgacgg
      121 aacacacgag tggtcatgaa atataccacc agcaacacga taataatgaa cgcaaacggt
      181 agataaaaaa gtttatctga tgcgttcaat aaattaccgc caggataata aaaatagatt
      241 tcgaacaatg cattatttgg cactgctgat gtctttgcaa tatactccgt atcgacagaa
      301 tcaataatat tataattacc ttcgctatta taaataatca gtttttcacg agcgagttga
      361 ttcttggcaa tgatctcatg accttgtttc aaaaatagaa cttcatcatt agcgctgatt
      421 tgatcaagat actcgttcag ataccgccag tttaaatcga catgaatttt atctaccgct
      481 aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga
      541 cgatcaaagc aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac
      601 cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc
      661 ggtccattta cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag
      721 agataatatt tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc
      781 attttcagaa tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc
      841 tttaatgtca gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata
      901 agttcaaagc ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatg
      961 atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc
     1021 tttatctttt cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc
     1081 tgtgccgctg caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca
     1141 aaatcagcag aaagttacaa cacttgctta gggcaataat tgcctgtgag gtgattaccc
     1201 tttcaagcaa tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa
     1261 ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca
     1321 gcgacatgcg gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct
     1381 ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc tggcggcaga gataatcgag
     1441 gtgccaatgc gcatggtgaa aaacaccacg atagacgaga cggtcccagg caagatatga
     1501 cgcaataaaa tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc
     1561 tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa acgcggggat ggaaaaaatg
     1621 gcgactgcga taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc
     1681 agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcaa ccgatcccac
     1741 cagccttcat aatatccagc caacaagccc agcaacgtcc cgatcgccgc accgataaac
     1801 acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg
     1861 tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg
     1921 tcataatcaa aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc
     1981 acaatcaata aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt
     2041 cgccagaatt catgccacgg tgtacgcacc tggtcaggtt tgaccagtgg catggcgttt
     2101 aacaccgcct gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt
     2161 taatggcggc gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa
     2221 gcagaatttc cgcctgaatc accgggtaat cgcgcatttc tacggagtca acgagtaagc
     2281 gtccaagtcc gggccagttg aaaactttct ccaccacgat agaaccaccg agcaaaaagc
     2341 caaactgtaa gcccatcatg gtcactaccg ggatcatcgc gttacgtagc ccgtgtttga
     2401 ggacaaccca ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta
     2461 aaacatcgac aaacgacgcg cgggtaaagc gcgccatcac ggctgccact gccgcgccga
     2521 gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc tcccacggta ggcagccagc
     2581 ccagttcaac ggagaatacc tgaattaaaa gcatccccag cgcaaatgcc ggaaacgaga
     2641 tccccgacac cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg
     2701 cgataattcc cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc
     2761 acagcgttgg cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg
     2821 acaggccaaa atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg
     2881 gctgatccaa ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgggcc
     2941 cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac aaataaaaac accagcaccg
     3001 agacgataaa cagcgtcgga atcaacccca gtaagcgttt gataacgtaa ttaagcattc
     3061 cactccctgc gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgg tgtctggcat
     3121 gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg
     3181 gatccacggc gactcttgcc agatgatatc ctgcgccgcc ttatacaagc gggtcttttc
     3241 cgccggatca ttagttttca gtgcctgagc caggaagtcg tccacctgtt tattgctgta
     3301 aaatgcggta ttaaacagcg tcggtggcca gttttgcgag gcgaacagtg gcgatagcgc
     3361 ccagtccgct tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc
     3421 tttttgccct ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg
     3481 ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt
     3541 gctgtggtta tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt
     3601 cagtaattcg cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc
     3661 gatacttggc ggtaccacac cagtagctgg cgttgcatag cccgcaaagg caactttcac
     3721 cagcgccgga cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg
     3781 cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc
     3841 gatatttttg tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg
     3901 cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt
     3961 tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac
     4021 aaaatcggtc tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc
     4081 aatctccttg ccatattttt ccagcgctgc cggtgaaatc atcgcggtcg ccggatgggc
     4141 aagaatatta ataaacgctg agaacggctg tttgagggta atctttactg ttgttggatc
     4201 gatcgcctcc gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg
     4261 atcgctggcc cggtccagat tcgctttcac cgccgcggcg ttgaaatcgg tgccatcctg
     4321 gaatttaatg ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata
     4381 actctccgcc agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta
     4441 aaacgatttc gctacggcct gagataacgt gtcattcgcg tcatacggat cgagcgtggt
     4501 gaaattcgat cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa
     4561 cgctgtcgca atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt
     4621 ctccgcctga atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat
     4681 gccccggccc gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat
     4741 tgctgggaag atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga
     4801 ccggaactgc cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc
     4861 gccgtggacc aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc
     4921 gctctaccac ggccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga
     4981 gatcgagcaa caagttgata atctgcccgc gaatagaaac atccagcgcc gagacggctt
     5041 cgtcggcaat gatcactttc ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc
     5101 gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac ag
//
LOCUS       DAFATA010000185         4933 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFATA010000185 DAFATA010000000
VERSION     DAFATA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.187"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..304)
                     /locus_tag="LGJ20_004623"
     CDS             complement(<1..304)
                     /locus_tag="LGJ20_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBK2388607.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVVVVRVAEHKEEDGKTQDQL"
     gene            462..1571
                     /locus_tag="LGJ20_004624"
     CDS             462..1571
                     /locus_tag="LGJ20_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBK2388608.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKTEHTIDDSGFTTQLELEAKIPEWIAETE"
     gene            1971..3110
                     /locus_tag="LGJ20_004625"
     CDS             1971..3110
                     /locus_tag="LGJ20_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014606071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBK2388609.1"
                     /translation="MGNNKVKLGRNDKCWCNSGLKYKKCHLGRDRETPVSKAEALKFS
                     KSLSQRKACYAPASMHEDCDKRIVQAHTISKSSSLKAIADSSNHVMGVIMNLASLMRN
                     NGKWLPEKIGINQASTFTGFCAVHDRILFSCLENEKFTGTDEQCFALMFRSLSKEIYA
                     KEGGVLSSDFAKNADKGKLQVEQYYIQKLIDQHQSGLNAAIIELNNLKTSLDRILLSR
                     QFSEIESVIFTFAEPLPIVVSSILSPERDFDGTKIQDLSDLTVSAEQVCFNAFSCGGK
                     GYVVFSWLRTSTIIRRFIRTLIKIPANRMFNTLLYFFFTKAENTYSSPEWWDRLSDKQ
                     RENIGNMIMSGVEFFGRPTSQVDHSVDYNAAALEEITCSNNEICS"
     gene            3400..3660
                     /locus_tag="LGJ20_004626"
     CDS             3400..3660
                     /locus_tag="LGJ20_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HBK2388610.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            3852..3992
                     /locus_tag="LGJ20_004627"
     CDS             3852..3992
                     /locus_tag="LGJ20_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBK2388611.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(4181..4462)
                     /locus_tag="LGJ20_004628"
     CDS             complement(4181..4462)
                     /locus_tag="LGJ20_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388612.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
BASE COUNT         1421 a         1174 c         1153 g         1185 t
ORIGIN      
        1 ccagttgatc ctgcgtcttt ccgtcttctt ctttgtgttc agccacgcga acgacgacca
       61 cctttgtgct cacctggtct gcgatggcct taagcgcacg ataaagcgtc cccgttgtcc
      121 cgcattttcc cagcacatca ttgacgcggg tcagcagtgt gggcttgttc agcgggaaca
      181 gctccgcgtc cgcatcatcc gccgttgcca cgataccgat aacgctggaa tcaacatcgt
      241 taatcgctgt taccaggtcg gtattttccg taacacgggc accatgaaaa cgagtttcac
      301 tcatagcttc agccccttgt atccgttaaa tgattcggca acaatcatca cccaccacgc
      361 gcgtaatctc acccctgcgc cgttctcccg acccggcgac aacaaaaagc agtaaccccc
      421 tctgcacgca catgcgacca tgccgcacag ggagggaaca gatgaccgac accaccatgc
      481 aattgctcag tcagggcaca gaccccgtga aaatgccaga ttttgatatt ctcgcggagg
      541 ggaaaacgct gtcaggcgtg gcagagcgcc tgatgagcct gtcactgacc gacaaccggg
      601 gatttgaggc ggaccagctc accatcacgc tggatgatgc ggatggtcag ttgcagctac
      661 cgccacgggg cgcgcgcctg acggttctca ttggctggaa aggagaaccg ctgacagaaa
      721 aaggcactta cattgttgat gaaatcgctc acgaaggacc gccggacagg ctgactgttt
      781 cagccagaag cgcagatttt cgggatgaat ttaacgttaa acgtgaagtg tcctggcatg
      841 atgtgaccgt tgagcgcgtg gtatccgcca tcgctcatcg gtacggcctg aaaccgcaaa
      901 tcagcgaaat gctgatggat atcgaaatcg accacgccga ccagaccgaa gaaagcgaca
      961 tgtccttcct cacgcgcatg gcggaaatgc tgggcgcaat caccacggta aaaagcggta
     1021 acctgttatt catcatgccc ggcggtggcg tgaacgcaca gggccagccg ttgccctcgt
     1081 tcgccatcac acgcagcagc ggcgatcgcc atcagttccg cattgctgac cgcgaagcgt
     1141 atacgggggt acgcgcttac tggcttgatc ttaattacgg gaaaaagaaa aaagtcagcg
     1201 tgaaacgccg caaaccgcca aaacccaaaa aggagaaaag cagcagccgt gaaggtgatt
     1261 atatggaagg cgcagaaggc aatgtgtttg tgttacgcaa gacttatcag aacgagcagg
     1321 cagcaagacg cgcagcggcg gcaaagtggc agcagctaca acgcggagcc gcatcatttt
     1381 ccatcacgct ggcgcgtgga cgtgcagaac tctaccccga aatgcatggc acggtaacag
     1441 gatttaaaag cgagattgat aatcaggact ggattattgc aaaaaccgag cacactattg
     1501 atgacagtgg ctttaccaca cagcttgagc ttgaagcaaa aatcccggaa tggatagcgg
     1561 aaacagaatg agcaaattag atgtattgac tcaaaccaga actgacacac tcacagcaca
     1621 gtgccagacc caatctggca gcccgctttg tgcctagagc aggtgttgct tatgtaacag
     1681 ttaatccacg gatagtggtc atgaatgctg gttgaagttg tattgccatg agtggtcaat
     1741 tagggactca cttattatcg cgtgctcacc attgatgggt aagatcatgc atttcggcta
     1801 ctgtacttcg cgaatcctcc acacacaggc aacacctgat ttaagaacag agggagatga
     1861 tgacgcaagg aagatttatt atcgtgcatg tggcagatga tcttttagct gccggagtga
     1921 taaattacac ctattttcga taagtgtttt tgttatagaa cattaatttc gtgggaaaca
     1981 ataaagtgaa attaggaaga aatgataagt gctggtgtaa ttcaggactt aagtataaaa
     2041 agtgccatct tggtcgagac cgtgaaactc cggtatccaa agccgaggca ctgaaatttt
     2101 caaagtctct ctcgcagaga aaggcatgtt atgcgccagc atccatgcac gaagattgtg
     2161 ataaacgcat cgttcaggcg catacgatat ctaagagcag tagccttaag gcgatcgccg
     2221 actccagcaa tcacgtcatg ggggtgataa tgaacttagc aagcctgatg agaaacaacg
     2281 gaaaatggct tcccgagaag atcggtatca atcaggcatc cacttttaca ggattttgtg
     2341 cagttcatga cagaatcttg ttttcatgcc tagagaacga aaaatttaca ggcacggacg
     2401 agcagtgctt tgccttaatg tttcgttcat tgtctaaaga aatttacgcc aaagaagggg
     2461 gagtgctatc ttccgatttt gctaaaaatg ccgataaagg aaagctacaa gttgaacagt
     2521 attatattca gaaattaatc gaccagcacc aaagtggctt aaatgcagca ataattgaat
     2581 taaataatct taaaactagc ctcgaccgca tcctacttag caggcaattt tcagaaattg
     2641 aaagtgttat cttcactttt gcagagcctt taccaattgt agtttcgtca attctttcac
     2701 cggagaggga tttcgatggg accaaaattc aggatttgag cgatcttaca gtgtcggcgg
     2761 aacaagtatg tttcaatgct ttttcctgcg gaggtaaggg atatgtagtg ttcagttggc
     2821 tgagaacttc aacaattatc agacgtttta tacggacact aattaagatc ccggctaaca
     2881 ggatgttcaa tacccttctg tattttttct tcacaaaggc cgaaaataca tattcatccc
     2941 ctgagtggtg ggacaggctc agtgataaac agcgagagaa tatcggcaac atgattatgt
     3001 caggggttga attttttggc agacctacat cacaggttga tcattccgtt gattataacg
     3061 ccgctgcact tgaagagata acatgcagca acaacgaaat ttgcagctga atgtttacat
     3121 gctgttagaa aaagcgggct gaacaatcat ttcgggattg taacagataa gatggcactg
     3181 cccgcttgct tcccgatatg tacctcagcc aataaactta tgtaagcaac cgatttccat
     3241 ttatgcggag gttatcaaag ttgttggagt tgcgaacttc cgctcttcgc acaaagcgta
     3301 ccgctaggtt tgattgtatt ctggctgcgc aaactgtcag ttggagtctg agctattaca
     3361 acttagaata gcggcagcac cacgttaagg gaggtcgcta tgttccgttg tccgctttgt
     3421 ggcgcatctg cccgaatccg caccagtcgt ccggaaaacg attcaaacac tgtgcggcaa
     3481 aagtattacc agtgtaacaa cctggaatgc ggcgtatgct tctcaacact ggaagctttc
     3541 cataaattca catcaaaaca cgcctccggc gttcactctt cggaaggtat cccgtggcat
     3601 gagctgccag cttcacacag gggaaacaat cagatgagtt tgcctttacc tcagaattaa
     3661 caggcagaat tgccggagta acaaaaaaac gatagattac ccgcgggtgc ctttcggctg
     3721 atggtcggag ggaatacccg aaggccggat gtggaaaggc cccggaaaac atttctgttt
     3781 aaccgaggcc ctaacatatc taccttaagc aagtgatagg ttagcgcctc tccaacaaag
     3841 gagcaagcgc tatgtcgcaa aaatcgctta cggccatcac gttctgcgtg acggtaatcc
     3901 tcatcatctg gatgctacac ggttcgctgt gtgaaatacg gatgagcttc tggggagcgg
     3961 agtttgcggc gttcttacag tgtaagcagt aaggaaaccg cgacggggga gcaatccccc
     4021 gtcaatcggt tgctaaggtt ggctgaagtg gcaccctatc tcacagacat gaacaacaaa
     4081 cccgcagcgt aagaactgcg ggttttcttt ttggaactat cactagctag tgtaccgtta
     4141 acggcacata atgctcgcac aacaaacctg ttaaaagaga ttactcagca ttctcctgct
     4201 tccattgccg gatcatttca tcggtaacat caccctcata acataccacg tcataccctc
     4261 cattacggct atatgcactg cgtccaccac atctactacc attccttgca tgattataag
     4321 gacacgcaca attgcctgga taagattcaa tggattcttt aattatttct tttttgatct
     4381 gagcatcaga ctttcctgtt gctgcatatc cactaaaaga tactaaacaa aggcagattg
     4441 ccattaataa accacatcgc ataccgatac actctattac taaccaatca tagcccacat
     4501 cataaaatcg agatgaggag cacaatacct ttaacaagaa ggtgtcataa cgataatatg
     4561 tcgtgcaatt catcaaaaaa aagatctata tcaaagagat agaactctta tcagtgcttc
     4621 agacatcggc attcgaggca gcagaaaaac agtaaatctg acgaatttgc cttttcccaa
     4681 gatgttgtca gattaaaaag atagattagc gcaggtacct gttgggggat tgcaggagga
     4741 aaaatgaagt cgagtggaaa aatcccagaa aaatttttgt ttaacagagg tcctggttca
     4801 ctatacgaaa tacggaggag ctgcagaaga attctgcttc atccttagag tttaaacagc
     4861 acgtgcacta ccactgtttc tgcatatcgc cacaccatcg ccactctacc gccattgcac
     4921 aaaatacaaa tac
//
LOCUS       DAFATA010000186         4926 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFATA010000186 DAFATA010000000
VERSION     DAFATA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.188"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            87..4349
                     /locus_tag="LGJ20_004629"
     CDS             87..4349
                     /locus_tag="LGJ20_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2388613.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPKKKD
                     SPNYGNPVNPVLGAKVLPGETDIALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEVSYSRSESFWLARGGVAEQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPPPPAYRVLTGVVDG
                     FGRTLTFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSSPAGP
                     RSASSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLA
                     AETSRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQMVSQKDAQGRETRYEYSAAGDLTATVSPDGKR
                     STIAYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTT
                     LSHTCEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYT
                     LTGQLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLD
                     IDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPE
                     GVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDL
                     TGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKAR
                     HRSLAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQL
                     EAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRL
                     PGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKF
                     HVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWN
                     SRASLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARV
                     ITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            4361..4810
                     /locus_tag="LGJ20_004630"
     CDS             4361..4810
                     /locus_tag="LGJ20_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388614.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT         1202 a         1161 c         1625 g          938 t
ORIGIN      
        1 aaataaaaat atatcgttaa tatatgttgg tgatgttttg ttgagggctt ttttattgtt
       61 agtggtataa atataaggga gtgagaatga gcggaaaacc ggcggcgcgg cagggcgaca
      121 tgacccgcaa gggactggat attgtgcagg gttcagcagg ggtgctgata ggcgcgccga
      181 cgggggttgc ctgctcggtg tgtcctaaaa aaaaggacag tccaaattat ggcaacccgg
      241 tgaacccggt gctgggtgca aaggtgctgc cgggcgagac ggacattgcg ctgcccggcc
      301 cgctgccgtt tattctttcc cgcgcctaca gcagctaccg gaccagaaca cccgcgccgg
      361 tgggggtgtt tggtcccggc tggaaagcgc ccttcgatat ccgcttacag atacgcgatg
      421 aaggcctgat actcaacgac agcggcggca ggagcattca ctttgagccg ctgtttcccg
      481 gcgaggtaag ctacagccgc agcgagtcgt tctggctggc gcggggcggg gtggcggagc
      541 agcacagttc gcagccgcta agcgcgctct ggcaggtgct gccggaagat gttcgcctga
      601 gtccgcatgt gtacctggcg acaaacagcc ttcaggggcc gtggtggata ctcagctggc
      661 cggagcgggt gccgggggcg gacgaggtgc tgccgcctcc gccgcccgca taccgggtgc
      721 tgacgggggt ggtggacggc ttcgggcgga cgctgacttt ccaccgggca gctaagggtg
      781 acgtggcggg cgcggtgacg ggggtgacgg acggcgcggg ccgccgtttt cacctggcgc
      841 tgaccacgca ggcgcagcgg gcggaagcct tccgtaaaca gcgcgccacg tctttatctt
      901 cccctgccgg tccccgttct gcttcctctt cttctgcttt ccccgacacg ctgcccgccg
      961 gtacagaata cggtgccgat aacggtatcc ggctggaggc ggtgtggctg acacacgacc
     1021 cggcataccc ggatgaacag ccgactgcgc cgctggcgcg ctacacgtac acggccagcg
     1081 gagaactgcg ggcggtgtat gaccgcagcg ggacgcaggt tcgcgggttt acttatgatg
     1141 cggagcacgc cgggcggatg gtggcacacc attatgcggg gaggccggag agccgctacc
     1201 ggtatgatga taccgggcgg gtgacggagc aggtcaaccc ggaggggctg gactatcgct
     1261 ttgagtacgg gcaggaccgt gtgaccatca cggacagcct gaaccggcgg gaggtgctgt
     1321 acacggaggg cgaaggcggc ctgaagcgtg tggtgaagaa ggagcatgcg gacggcagca
     1381 tcacccgcag cgagtatgat gaggcgggga ggctgaaggc gcagacggat gcggcgggac
     1441 ggcggacgga gtaccgactg catatggcgt ctggaaagct gacttcagtg atactgccgg
     1501 atggcaggac ggtacggtat ggctataaca gccagcggca ggtgacgtcg gtgacgtacc
     1561 cggacgggct tcgcagcagc cgggagtatg atgagaaggg aaggctggcg gcggagacct
     1621 cacgcagcgg tgagacgacg agttacagct atgatgaccc ggcgagtgag ctgccgacag
     1681 ggatacagga cgcgacgggc agtacaaaac agatggcatg gagccgttac ggtcaactgc
     1741 tggcctttac ggactgctcg gggtacacga cgcggtatga gtacgaccgg tacggtcaac
     1801 aaatcgccgt tcaccgggaa gaaggcatca gcacttacag cagttataac ccgcgtgggc
     1861 aaatggtcag tcagaaggat gcgcaggggc gtgaaacccg ttatgagtac agcgccgcag
     1921 gagacctgac agcaacggta tcaccggacg gtaaacgcag caccatcgca tatgataaac
     1981 gcgggcgtcc ggtgagtgtg acggaaggtg gactgacgcg cagcatgggg tatgacgctg
     2041 ccgggcgcat caccgtgctg accaacgaga acggcagcca gtccacgttc cggtatgacc
     2101 cggtggacag gctgactgaa cagcgcggtt ttgacggccg gacgcaacgt taccactatg
     2161 acctgaccgg aaaactcacg cagagtgaag acgaggggct tatcaccctc tggcactacg
     2221 atgcgtcgga ccgcatcacg caccggacgg tgaacggcga cccggcagag cagtggcagt
     2281 atgatgagca cgggtggtta accaccctca gccatacctg tgaaggccac cgcgtgtcgg
     2341 tccactacgg ctatgacgat aaaggccgcc tgacgggtga acggcagacg gtggagaacc
     2401 cggagacagg ggagatgctg tgggagcatg agacggggca tgcgtacagc gaacaggggc
     2461 tggcgacccg tcaggagccg gacggtctgc cgccggtgga gtggctgacg tatggcagcg
     2521 gttatcttgc ggggatgaag ctgggcggaa cgccgctggt cgagtacacg cgggaccggc
     2581 tgcaccgcga gacggcccgt agcttcggcg gggcgggcag tacagcgggg tacgaacagg
     2641 ccacggcgta caccctcaca gggcagctac agagcaggca cctgaacctt ccgcagcttg
     2701 actgtgacta tacgtggaac gataacggac aactggtgcg catcagcggc ccgcaggagt
     2761 gccgggagta ccgttacagc ggcacgggaa ggctgacggg cgtccacact acggcagcga
     2821 acctggatat cgatatcccg tatgcgacgg acccggcagg aaaccggctg ccggacccgg
     2881 aactgcaccc ggacagcacc ctcacggcgt ggccggataa ccgcatcgcg gaagatgcgc
     2941 actatgtcta ccgctacgat gaatacggca ggctggcgga gaagacggac cgcatcccgg
     3001 aaggggtgat acggatgcac gacgagcgga cccaccacta ccactacgac agccagcacc
     3061 gtctggtgtt ctacacgcgg atacagcacg gtgagccgca ggtggagagc cgctacctct
     3121 atgacccgct gggccgccgg acgggaaaac gggtgtggcg gcgggagcgt gacctgacgg
     3181 ggtggatgtc gctgtcgcgt aaaccggagg agacctggta cgggtgggac ggtgacaggc
     3241 tgaccactgt acagacccaa cagacaagaa tccagacggt ataccagccg ggaagcttca
     3301 cgccgctcct gagaatcgaa acagagaatg gtgaacaggc gaaggcgcgg caccgtagcc
     3361 tggcggaggt gttgcaggag gacacgggtg tgacgctacc ggcggagctg gcggtgatgc
     3421 tggggaggct ggagcgggaa ctgcggcagg gcagcgtgag tgaagaaagc cagcagtggc
     3481 ttgcgcagtg cgggctgacg gcggaacaga tggccgcgca actggaggcg gaatacatcc
     3541 cggagaggaa acttcatctt taccattgtg accaccgtgg gctgccgctg gcgcttatca
     3601 gcccggaagg ggaaacggcg tggcaggggg agtatgacga gtggggaaac ctgctgggcg
     3661 aggaaagcgc gcagcacctt cagcagtcac tccgtctgcc gggacagcag tatgatgagg
     3721 agtcggggct gtactacaac cgcaaccggt actatgatcc gttgcagggg agatatatca
     3781 cccaggaccc gatagggctg agaggtgaat ggaatctgta taaatatcca ttaaaccctg
     3841 ttcggtttat agattctttg ggtttaaaat tccatgttaa cggtgatcca agtgatttta
     3901 atcaggctgt tgagtattta aaacaagatt cacaaatgaa agaaactata gattttttat
     3961 caagctctga agaaactata aatatagagt atattgaagg aaccaatgtt agatttaatt
     4021 cgaataatat ggcgatatat tggaatagta gagcatcttt attttgttca actgaattaa
     4081 actcgaaatc acaatcgcct gcattaggtt tgggacatga gtttgctcat gcgcagtatt
     4141 accttcttga taaggaaaat ttcatggctc ttttaagccg aacagataag aaatatgaga
     4201 ataaagaaga ggcaagggtt attacaataa ttgaatcgcg tgcggcgaaa actttaggtg
     4261 agtgtaccag aggagctcat agtggactgc ctttttatcg ggtggatgga ccgttgcaga
     4321 cgatgaaaat aacgggtacg ccagaatagt gaggtttttt atgatgaaat atttgatggt
     4381 tttattgtct ctattttctg gttcggtact ggggatgggg cgagtgaatg aattgtgtgg
     4441 aatagatagc gtgaaaacga tagagataat taatcttcca tcgtatgtta ccacgttagt
     4501 tccgcttagc aaggaaggtc ttaatgaaat atacagatat aaagtggtgg tgaatgaaat
     4561 tagcgatctt tatgctggta agataattga tttattgcaa atgaagtatt tcagaaaaga
     4621 aaaatataac aatataagat ggggggttag tatcatttcg aaaggtaata ataaatgtga
     4681 aatttatttt gatgcttttg gtgaatgtgg aagtgtgaat ggcattaacg tgtgttttga
     4741 aaaaaatgaa atgataggtt ggattaaaaa agaaatccct ttgctgtcgc aaaaaatagg
     4801 tgggctttaa atggtagggc aatattcaaa gacagtatag gtaactattt gtatggccat
     4861 gtaaggtaaa agaaaaggaa taaaccatga gcggaaaacc ggcggcgcgg cagggcgaca
     4921 tgaccc
//
LOCUS       DAFATA010000187         4789 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFATA010000187 DAFATA010000000
VERSION     DAFATA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.189"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..230)
                     /locus_tag="LGJ20_004631"
     CDS             complement(<1..230)
                     /locus_tag="LGJ20_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="This protein is required for primosome-dependent
                     normal DNA replication; it is also involved in inducing
                     stable DNA replication during SOS response. It forms, in
                     concert with dnaB protein and other prepriming proteins
                     dnaC, N, N', N'' a prepriming protein complex on the
                     specific site of the template DNA recognized by protein
                     N'; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK2388615.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEP"
     gene            complement(337..810)
                     /locus_tag="LGJ20_004632"
     CDS             complement(337..810)
                     /locus_tag="LGJ20_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBK2388616.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(801..1571)
                     /locus_tag="LGJ20_004633"
     CDS             complement(801..1571)
                     /locus_tag="LGJ20_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBK2388617.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            2190..2915
                     /locus_tag="LGJ20_004634"
     CDS             2190..2915
                     /locus_tag="LGJ20_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK2388618.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            2873..3550
                     /gene="bglJ"
                     /locus_tag="LGJ20_004635"
     CDS             2873..3550
                     /gene="bglJ"
                     /locus_tag="LGJ20_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBK2388619.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(3588..4376)
                     /gene="fhuF"
                     /locus_tag="LGJ20_004636"
     CDS             complement(3588..4376)
                     /gene="fhuF"
                     /locus_tag="LGJ20_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBK2388620.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            4517..4753
                     /locus_tag="LGJ20_004637"
     CDS             4517..4753
                     /locus_tag="LGJ20_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBK2388621.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT         1306 a         1069 c         1182 g         1232 t
ORIGIN      
        1 ggttcctgat agagttgatc gtccagagcg acatcgcttc ccggacgcgc cagcttttct
       61 tccagcgcca gcagttcagc caggcgtgca ggcgtgacgg cataaaacgc cggggcattg
      121 ttagcaaata cggcaaccac accgccttca gcttttgcca gaacggtttg gtgatcgtgt
      181 accagggcgt caataccaac gacgtccggg gtcaaaactc tggaagacat actgtttctc
      241 aaatatggaa cgatgaacga gaagggagat aacttttata gtaacacacg gaggagagga
      301 ggctgtctct catccgtgtg cttaagcgag gcaattttat acgcgagggc gcttgcggta
      361 caaccataat ccaggaatgg aaagaccgat ggataacgca ccaacaatcg atgaagctgt
      421 aagaaagttg gttaacaggg taatcatcaa cggttcgctg taacctaact ggctgatttt
      481 taccgccgaa atcattgcgg tataagccga tatgcccggg aacataggga taacggccgc
      541 cacggtaaac actttcggat gcgccagata ccagcgcgac cattgaatac caatggtgcc
      601 gaccagcata gaagccataa aagttgacca ctcaatattc agcccgctgg tcattaagat
      661 cattcgtgaa ccatgaccaa tcgcgccaag cagcgcacac cagcgtaacg cccgaacggg
      721 aacgttgaac accatcgcaa agccgaccgc aggaatggcg gcgaggatca tatcctgcgc
      781 caacgctaac agaaattcga tcacacccat ccgcgtagcc cccaaatcgt cagtgccatc
      841 actacgccga cgcaggtagc cagtgtcagc agactggcga tcgcccagcg tgccagtccg
      901 gtattgatgt ggcctttaaa catatcggcg acggcattaa tcaacggaaa tcccggcacc
      961 agcagcagaa cgctggcggc catcgcgata gtgggtgtat tgctgaaagt gggaagttgc
     1021 aaaagcaatc cggaaatggt ggtggcggcg aaagcggtaa ggcaaaagtt gatttgtgga
     1081 tgaagatgac gttgtgccag cagctggcgg atatacatcg cggccgtact ggcaaagaag
     1141 gtgatgacgg caccatccca gccaccttta ttgagtttac agaaacaggc acaagaaagg
     1201 ccaaccatta aggctaccag ccatcttggg taacgtaatg gctgaatttg gctaaatcgt
     1261 ttctctacgc ctttgtaatc cagcagatgg tgctccgcca gaatcacaat gtgctggact
     1321 tcagtcacca catgcatatt aatgccgcga tcgtgatttt tacgtgtcga tgtcaggcat
     1381 tgcccatctt taatggttgt cagcactatg gcgttcgaag agatagaact ttcgacgctg
     1441 tccatcccca acgcccgacc cagtcgtgag gaaagctcat caaccaacgc gctttccgca
     1501 ccatgttgca aaagaaataa tccacactgg atacaaagcc gtgttacggc tcgctgttgc
     1561 tcagtttgca taactcgccc tttgtcatag acgtgctgcg tattgttcag ataatgagaa
     1621 tccatttgta tcatgaagca cggttgtttt gcggtaggtt aagatcaaaa ttgcgctgat
     1681 taatcctggt tatcgggaca agctcggtga taaaaagcga attatctctc gccgcaaaca
     1741 aactatcaca tgaatattat cactataatg aatttattgt ttggccttta cgaatcagga
     1801 taatagataa ccgggcacgt tttttaatgt taacaagtta aaaccatcag atttcattga
     1861 ataaatattg gttgtgtgat ttttcattct gcgggaaggg atatcattta attataaatt
     1921 aaatgaatgt gatttctgtt tttcattaag tattcctaac aacttaatgt agatatttta
     1981 aatgtctcca ggctatttca ccaggttatc atttgatgct aaccttctga cacatgcagt
     2041 ggagttgttg tgcagcagga gtatgctgat atgaaagtga atgctaagga taatttattc
     2101 gcttaatcta ttaatttgct ggaaacattt aaggaatgaa tatcctgaat aaaccatcat
     2161 gatttttaga cagtggatgt ggaggaaata tgttgccagg atgctgcaaa aatggaattg
     2221 ttatcagtaa aatacctgtt atgcaagcag ggttaaaaga ggtcatgagg actcacttcc
     2281 ctgaatatga aataatatcc agcgcctctg cggaggacct taccttatta caattacgtc
     2341 gttccggatt agtcattgct gatttagccg gtgaaagtga agatcctcgt tctgtttgtg
     2401 aacattatta ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac
     2461 gctcctggta ttcccaggca gtagaattgc tcatgtgccc tacggcgacg ttattgtctg
     2521 atgttgaacc cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc
     2581 gtatcagcgc catgctgacc tctccggcaa tgaatgaaac tcatgatttt agctatcgct
     2641 ccgtcattct cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca
     2701 tcaaccagat agcttcgttg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca
     2761 gtgcgatgcg tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg
     2821 cgcagggtgc aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca
     2881 cagccgaatt aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gcagtatcgg
     2941 tattgagagt ttattcagaa agtttgcggg taacccttat aagctccata cctataccag
     3001 tcaggagtca tttcaggatg ccatgtcgcg gatctcattt gcggcggtca ttttttcttt
     3061 ttctgccatg agaagtgagc gcagagaggg attatcttgc ctgactgaac tggcgattaa
     3121 gtttccgcgt acccggcgtt tagttattgc ggatgatgat attgaagctc ggctgattgg
     3181 ttcattgtcg ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt
     3241 tcatcaggaa ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa
     3301 tcagtggtac attaaccaaa gccggacgct aagcccgacg gagagagaaa tattgcgctt
     3361 tatgtcgcgt ggctactcaa tgacacaaat tgccgagcag cttaaacgca atatcaaaac
     3421 gatccgtgca cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt
     3481 gttggaggcc gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca
     3541 tccctattaa tctgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta
     3601 caatcgccac attgctgcac atccggtaag cgataacgct ggcagcaggt ccggcgcacc
     3661 agcaggccgt cgcgtagcac cacggtacgc cagagtggat tatcttcacc ggtagtaagc
     3721 gttttctcaa aaaagagggc atggcgcagc gattcaaccg tcgcctcgcc gagcagttgc
     3781 ttcatctcag taagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg
     3841 ttgatctctc cggtcgcttc tagtgcctgc acaaccggaa ccagcgcctg gctgattaac
     3901 ctttccattc gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc
     3961 cagaaacagg cggcacggcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca
     4021 tctaatgcct tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata
     4081 taccattgtg cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg
     4141 cgatagatat gatcggaata gaccgcgagt agagaactta gcgcattcgg tgatgaccat
     4201 tgcgccagcg tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa
     4261 tgttcacgat gttcggcgat cgtcgcacgt acggcttgtg caagcccagc atcctgtggc
     4321 tggagatgcg ttcgccagat aacatcttca tagagcggtg cggaacgata ggccataatc
     4381 gggatagtaa tctaaatgat aatgattgct aatcctagcg ataggtttac ccgatagcaa
     4441 gggatttctc tggcttgcaa atgataaaaa ttatcatatg atattggtta tcattatcaa
     4501 tgaaagagat gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc
     4561 aggattgctg attgcagggc tgatgtatgc ggatttatca ccggatcagt ggcggattgt
     4621 cattctgatg ggattagtat tgacgtcggt gatgctgtat cacaaacagt tacggcatta
     4681 cgttttgcta ccatcgtgcc tggcgcttat tgctgccatc atgctgatga taatgaattt
     4741 gaatcaggga tgaaaaatca aggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DAFATA010000188         4681 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFATA010000188 DAFATA010000000
VERSION     DAFATA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.190"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..140)
                     /locus_tag="LGJ20_004638"
     CDS             complement(<1..140)
                     /locus_tag="LGJ20_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HBK2388622.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQ"
     gene            complement(140..634)
                     /locus_tag="LGJ20_004639"
     CDS             complement(140..634)
                     /locus_tag="LGJ20_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBK2388623.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAI
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(738..1538)
                     /locus_tag="LGJ20_004640"
     CDS             complement(738..1538)
                     /locus_tag="LGJ20_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK2388624.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLMQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(1584..2636)
                     /locus_tag="LGJ20_004641"
     CDS             complement(1584..2636)
                     /locus_tag="LGJ20_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBK2388625.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENAVKESTEFTKKINVFGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRVSTQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRAIG
                     GLDVFLAPFFPDSTMLITSFNNLSIYWQKGTMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(2660..3496)
                     /locus_tag="LGJ20_004642"
     CDS             complement(2660..3496)
                     /locus_tag="LGJ20_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBK2388626.1"
                     /translation="MANEKKTSRKKFRVAVSGVTADGREINGDMLKAAATSYNPSVYG
                     ARVNIEHILSPLPGSEFSAMGDVVGLSTEDITDGPLAGRTALYAEIEPTARMMSLLND
                     GKKIYSSIELEPQSTITGGPYLRGLAMTDTPASLGTERLAFAAQQRMQLMTFNCQQGD
                     VAMFTAAMESELIELTEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            3651..>4681
                     /locus_tag="LGJ20_004643"
     CDS             3651..>4681
                     /locus_tag="LGJ20_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HBK2388627.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDI"
BASE COUNT          969 a         1292 c         1314 g         1106 t
ORIGIN      
        1 tcctgcccgg cgtccagaaa tacctgctga cagagtccgg ggttggcatc cagcaccttt
       61 tcggtgacgc cctgcgtcgt gccgtagtac cggaaacaga gcgaatccac ggtgtcgcct
      121 tccagtgcct tcactttcat cagcacagct ccgcaaaaat tcgcgggcgg cacagaatgt
      181 cagagatggc ccagctcaca tcgcgccaca aatctgatgt ctgtatatcc agtgcgtccg
      241 cccggcggtc gcccttgtcc gttgtgtccg catcgcggta acgctccaga atcagggcgc
      301 gtgtggcggt ataaacagca ttgcgccagt gccagagatt gacgctttct ccgttaatta
      361 cgggtgccgg aacatcggcc agcgtctgat ggccagccgc ctgctgttcc tgctgccatg
      421 cttccagctc gcgggtaacg tgagccaccg ccccggtggc ggtatgcagc aggcgggagg
      481 tggtcacacg gcccggcagt cgtatcgcca gacgcagctc acgcagcaca atatccggcc
      541 agaatgcgcc cgctgaaata cgggtatcac catcatcggt atcggtgatg tcgtcctctg
      601 cgggtccggg gttggttctg gcaaccatac tcatggggtt cactcctgaa aaaaaatcgg
      661 gcggtgggtg cgcggtgtaa acggtcacgg agtcaaaccg gaacaccgcg tacgccgccc
      721 gctgacgggg tcagtcgtta accgcgcttc gccttctgcg tcgcggtggt ttttcgtgtt
      781 gcaggcttcc gcgttgtctt tttacttttg ctgctttcgt cctgcgcctg ctgtgcgctg
      841 gcgtcttctg gtgctgctgc ggaatcggct tttttcaggg cgcgggaaag ggttgcaatc
      901 tcgcgtttca cacctgcgtt cgggttcagg tgcattgctt cgcgcagcag cttcaatgac
      961 agggccatgc tgtccgcatc gctcaggcca cggcgggcaa aggcgcacgc cttgcataat
     1021 ttggcgcgca cttcgtccgg catgtcctgg tcggtgacaa tctcccggag ggtatccagt
     1081 ggttcgataa aggcggacaa atccgcgtcg gcatccgtcc cggcctgcat cagtaccggg
     1141 ttgcagattt cttcggtcag cactgtggca gcagtacggc caaagttatc cggcataatg
     1201 aggttatgac ggaccacata cgcaccaata cgcaacgcca gcggaagatc gccgcagtca
     1261 atcgcccaca ccatcagcgt ggcaatcact tcatcctgct gcccgccgtc agcctccagc
     1321 gttccctcaa tccagccgga aaaatccggc aacaactctt ttttgatggc ggctttcgcg
     1381 cttctggcct gtacgccctt aagccgggcc tgtgccagac gcagacgata cagcacctct
     1441 tcatgcgcgg tacgcgcggc gtggtccacg ccttcattcg cccggcctgc gcgctgtgcc
     1501 atcacgttct gccagtgttg ctgtgcagga gtaatcattt tttctctccg ttacaggcgg
     1561 gcatgatgcc cgccgtgagt tgattagctg tcggcgaact tcaggccagt gaccatcgcg
     1621 cacttgccat agtcttcaac gacataagcg tcattgatgg actggtaggt ggcgatgcgg
     1681 ttgtattccg gctcgtcttt catcaggcga cgcattgttc ctttctgcca gtaaatcgac
     1741 aggttattga acgaggtgat cagcatcgtt gaatccggga agaacggcgc aaggaataca
     1801 tccagcccgc caatggcgcg cgatgacagg atgagctgtc cggcaagtaa ttccgcattg
     1861 ggattctggc cgctgatgct gttcagcacg ggcagacgca gcgagttaaa caggttgcgc
     1921 cccataatca ccacgaggtc gtcagcttcc ttgtgccatt catccagcag ggatgagcgc
     1981 gcgtcctgta ccagtgcatc agcgttcgcg tatttacccg cgtgcgccac ggtgttgtcc
     2041 atgttgcggg aagttagcgt cacgtcattc attacgcgct cgctggcatt ggttctgatg
     2101 tgctccagcc accccacgtt aacgtcctga agcagcttgt tggtgctgaa gttggactca
     2161 tccgcgtgag acgtgccgtt gaaaccgatc atgatgcggt caagcgccac ctgccgggca
     2221 atctgtgtgc tgacgcgtga ctgaaaatca gggtgtgccg cccaggcatc aagctgcgga
     2281 tacgaaataa acgtgtcgta gttcacctgt tcgcactgat atttgcggtt tttcagatca
     2341 accacgttat tcgggttacg gcgttttgtg ccgtcataac tggtattcgt gcgcgcaatc
     2401 ggcccggtgg tgtccaggag gattttttcg cctttctggt cggtcacacc gaacacgtta
     2461 attttttttg taaattcagt gctctccttt actgcgtttt caaaacgctg ctgcaccgag
     2521 ggttccacgg taaatcgcga taccagtgcg gaaaccggga tattgttaag cgacgcctgc
     2581 tgcgccatat agcaacccag cttgttgcgg gtaatatctg acatcaccag attcataaaa
     2641 aatttgctcc tttgtcttat cagaagtcag ccagttgatc ggaggctgcg cccgttgcgg
     2701 tgaaccggtt ctgcggatcg ccgtcctgcg tgcgcagttt ttccttcagt gctgtcagct
     2761 ctgtggtcag taaagtgatt ttctggctgt cctgctgatg gcgggtttcc agcgcattaa
     2821 aacggtcgat aatgtcggcc tgtgacgttg cgacaccttc caccgcttcc tgaatacggg
     2881 agaaactggc gtcatccgct ttgcggccac ggccaataat ccccataacg cggttaaacc
     2941 actgggtgcc ttcttcctga cgttgttcgg tgagttcgat aagttctgac tccatagcgg
     3001 cggtaaacat cgccacgtct ccctgctgac agttgaatgt catcagttgc atacgttgtt
     3061 gtgccgcaaa ggccagacgt tccgtgccca ggctggcggg ggtgtcggtc attgccagcc
     3121 cgcgcaggta agggcctccc gtgatggttg actgtggttc cagctcaata ctggagtaaa
     3181 tttttttacc atcgttaagc agggacatca tgcgagcggt cggctcaatt tcggcataca
     3241 gtgccgtgcg acctgccagc gggccatcgg ttatgtcttc ggtgctcaac cccacaacat
     3301 cgcccatagc ggaaaactcg ctaccgggga gtggtgacaa gatgtgctca atattcacac
     3361 gtgcaccata aacggacggg ttataactgg tggcggcagc tttcagcatg tcgccgttga
     3421 tttcgcgccc gtctgccgtt acaccggaga cagccacgcg aaactttttg cgggatgtct
     3481 ttttttcatt agccatagtt tttgcccctc tgactggttc ttcagtcatg atggcaaagc
     3541 gtaacaggct gatacaaagg gcttttgttg taagaaaacg gccagaacag ggggttaagg
     3601 agaacggttt cgcgcgcggg taatcttcct gtaattactc agggggagca atgattcagg
     3661 acgcttttgt gcgccagcgt gcgcggcaac tttactggca gggttatccg cccgcagaaa
     3721 tatcacgtct gatgggaata aacccgaaca cgatttatgc gtggaaaaaa cgtgaccagt
     3781 gggatgaaac gccacccgtg cagcgtgtca cgcagtccat cgatgcgcgc ctcatccagc
     3841 ttactgaaaa acagaataaa acaggcggtg actttaagga aatagacctg ctgacccggc
     3901 agcttaaaaa gctgcatgat ggccagccgg atgtgatggc cgcaggaaag aaaggccggg
     3961 cgaaaaaact caaaaatcat ttcacgccgg aacagattgc cgcactgcgg gaaaaaatca
     4021 tcagcaggct ggagtggcat cagcggggct ggtttgactc cctgaccctt tgcagggaag
     4081 ccgggatacg taacaggatg atcctgaaat cccgacagat tggggcgacc tggtattttg
     4141 cacaggaagc actgctgatg gcgctgcgtg acgatgtggc gcaaccttac cagcgtaacc
     4201 agattttttt gtctgcgtcg cgtcgtcagg cgttccagtt taaaagcatt attcagaagg
     4261 ccgcggctga agttgatgtg gagctgaaag ggggcgataa aatcatcctc tccaacggcg
     4321 cagagctgca ttttctcggc acttctgctg cgtcggcaca gtcctatacg ggcaattttt
     4381 attttgatga atttttctgg gtcagtcgct ttgctgaact gcgcaaggtg gctggcgcta
     4441 tggcaaccct cagcggactg cggcgcacct acttctccac gccatccacc gaaacgcacg
     4501 aggcatacgc ctactggaac ggcgaccgct ggaacgagaa aaaggcctcg cataaacgcc
     4561 agcgtttttc tgtggactgg aaaacgctgc ataacgggct tatctgccct gaccggacgt
     4621 ggcggcaaat tgtcacgctg gaagatgtgg ttaatcacgg ctggaaacac accgatatcg
     4681 a
//
LOCUS       DAFATA010000189         4593 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFATA010000189 DAFATA010000000
VERSION     DAFATA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.191"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..607
                     /locus_tag="LGJ20_004644"
     CDS             <1..607
                     /locus_tag="LGJ20_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388628.1"
                     /translation="KNISYSLMAAFIFDTGLNFSKENITKGVISTRWHNDLYSSFERM
                     KAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFRLMWDLSSEKYLSYKEII
                     IRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPSLKTYRALSRLVNSIKD
                     PSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
     gene            complement(1295..1528)
                     /locus_tag="LGJ20_004645"
     CDS             complement(1295..1528)
                     /locus_tag="LGJ20_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBK2388629.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNN
                     AILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
     gene            complement(1535..2095)
                     /locus_tag="LGJ20_004646"
     CDS             complement(1535..2095)
                     /locus_tag="LGJ20_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBK2388630.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLN
                     RQKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPAL
                     TALSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKF
                     LVLPDGVKGENTWTVGDKNAVRLVRE"
     gene            complement(2070..2783)
                     /locus_tag="LGJ20_004647"
                     /pseudo
     CDS             complement(2070..2783)
                     /locus_tag="LGJ20_004647"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2773..3096)
                     /locus_tag="LGJ20_004648"
     CDS             complement(2773..3096)
                     /locus_tag="LGJ20_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK2388631.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPT
                     LSPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQG
                     GRDDS"
     gene            3159..3350
                     /locus_tag="LGJ20_004649"
     CDS             3159..3350
                     /locus_tag="LGJ20_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBK2388632.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGESN"
BASE COUNT         1106 a         1264 c         1054 g         1169 t
ORIGIN      
        1 aaaaaacata tcatattcac tgatggctgc atttattttt gatacagggc ttaattttag
       61 caaggagaat ataactaaag gagtgatttc aacaaggtgg cataatgacc tatattcatc
      121 atttgagaga atgaaagcaa ttaataaaat ttactaccca tccaacaaag agataaacac
      181 agaaggtcta tccaaagcca tcacatcatc tcttttcaat gatgacgcga actcgttcgc
      241 aaagagagat tttcgcttga tgtgggactt atcttctgaa aaatatcttt cctataaaga
      301 aataataata agaaaaggcg ataagcttga cgctgtatgc ttgagattta taaatgatga
      361 ctacaagttt ttagttaatt tcaacagaga tgaagaggtt ttcaaatact ttccatctat
      421 aatgcaacct tcactcaaaa catatagagc gttatcaaga ctagtaaact caatcaaaga
      481 tccttctaga tttaagttca cgacagagtc attagaaatg gagttactag aatacttaga
      541 gttaagaaat gaattattca atgacataga agaagtaatg ggtagctatg ctcagagggc
      601 accatagttc atgatagccc tcgatagtac ttacttatat ttttagagag gtttaagtgt
      661 aagtagaggg agtaacacct ggtttttcag catccactcc aatgaatgct gacaccgata
      721 cgcgataatt ctctttatcg tagcaaaatc atcctccaga ttgcggttga gaataggttc
      781 atggtgcgca atacgattgc gcagttttct cacgggactt gttagcagta atgcttcccc
      841 ccggggacaa acgacagaga tatacaacaa aggcccgcaa ttaaaatact ccgtatttac
      901 cataacgtcg cattgcgcgt accgcttaaa cgcgttcagc gcgatcacgg cagcagacag
      961 gtaaaaacag caacaaacca cccgaaaaac tgccgcgatc gcgcctggta aattttaacc
     1021 tcatgcatag ttatgcgcga aactgaatga cgatcagttt cgcacgttcc agccgtcgaa
     1081 cggggcaaaa tggggaggtt tggatattgt taatgctaat gtacgtacat taacattggc
     1141 gccatccggg tagggcgtgc cacaaactgg ctcgatttca gggggtaccc cggtcacatc
     1201 cccattgacg ggccacgcca gatccgcgct tcgggttcct gctcttcatg ccagcgccgg
     1261 gcaagctcct gcgcctgacg ctcctgttca tcccttatct gttgttcgtg ataaataacc
     1321 gactcaagcg gtcgacttgt ccggctaata gctgcacctg ctgactcaag ttcacgacac
     1381 gctcgttcag tatcgcgttg ttctgttgcg taaccgcgtg catcttctgc aattctgcga
     1441 aggcgctctc ccactcgttc agccgccgca tatagtcctg ttgcaaatgc tctaaggcgt
     1501 tcagcaaatg cccttccagt tccgtcatac tcgtttactc cctgaccagc ctcacggcgt
     1561 tcttgtcgcc caccgtccag gtgttttccc ctttcacccc gtccggcagc accagaaatt
     1621 tcccgttctc gccattgacg aacgtcaccc cccaggtttt tgcctcgagt ttcgccagcg
     1681 tgttttcctg ctctctgatg gtcaagatat ttttcgcaat cctgctttcc tgccactgaa
     1741 tcagccccca gcaccccagg aaaatgccgc ccgtgacgct gagcgcggtc agcgccggaa
     1801 acagtacccg ccctttgatc acctgccaga gcaggttttt ctgctgccgg gacagttcgg
     1861 cctctgtggt gctgagctgc gtgctcacgg tggtgctcag cgtatccagt tgctgtttca
     1921 cggatgttgt atgttcgctg atagcgtctc tgattttctg cctgtttaag ctcagtgcct
     1981 tgtctacaga ctcttctagc ttcctgaact cgctgttcag catgttctcg gtagaagcgg
     2041 ctcgctcttt cagtttcttc tcgaagtccg ttcccatttg taaaagattg ctcatacagc
     2101 gcccccttta gcctgagatt ccgccccccc tccgggtcgg caatactgat actgcttctg
     2161 gttgtcctca ccacctcaaa acctgccgct gtgagcgcct cagtgacatc ctgacgcgtt
     2221 tttattgctc cggcacggta aagcgcctca agaccccgtg taatcgcttc tgcggcctcc
     2281 tgtttcgctt ttggcagatt attcggtgtg acaagcgtcc gtctgttctc cggtgcgttc
     2341 gggtcacgca gcccgtaatg atgattaacc agcgtctgcc aggcatttat cctcggcctg
     2401 tccgcacggt cgtagtacgg ctggaggcgc ttaccgctct gtagttccat gttcggaatg
     2461 acaaaattca gttcgagccg ccccttatcc tggtgttcca cccagagaat gctgtactgg
     2521 tctttttcga gaccgggcat aagaacacgc tcaaagctcg tcatcactct ttcgcgttca
     2581 cccggcggta actccttctc cgcgaacgac agaacgcctg acgtgtattt cttcgaaaat
     2641 ggcgtggcat caatgagttc ccggatttct tccgggttac cccggagcac cgttgcgcct
     2701 tcgcggttac gctcccgccc cagcaggtaa tcgaccgggc cacttccacc gcctttcccc
     2761 ctggcatgaa atttaactat catcccgtcc cccctgttcc ctgacagcct gccgcaactg
     2821 gcgcaattca ccctcgatgg ccatcagagc ggccaccaca tgaacccggt caatcgaaga
     2881 ccagtggccg ctgttcacct tccgggctgt ctggttcaga ttgttcccga tggcagccag
     2941 atgccgcagc aatggcggcg atagtgtcgg cagttttcct gtgcgcgcaa ccggctcccc
     3001 cagacagacc cgccgcatcc attctgccag tcgtttaccc tcgcagcgtt ccagtaaccg
     3061 ggcgtgctcc tcatcgctaa cccgtatcgt gagcatcttc tcgcgtttca tcggtatcat
     3121 tccccctaaa aaatccccta ttaaggagcc ttaagaccat gaccaaacag gaaaaaacag
     3181 cccttaacat ggcccgcttt atcagaagcc agacgctgac cctgctggaa aaactcaacg
     3241 agctggacgc ggacgaacag gcagacatct gcgaatcact tcacgaccac gccgacgaac
     3301 tctaccgtag ttgcctcgca cgcttcggcg atgacggtga aagcaactga aacatgcagc
     3361 tcccggagac gttcacagct tgcctgtgag cggatgccgg gagcagacaa gcccgtcagg
     3421 gcgcgtcagc gggttttagc gggtgtcggg gcgcagccct gacccagtca cgtagcgata
     3481 gcggagtgta tactggctta actatgtggc atcagtgcgg attataagaa aagtgcacca
     3541 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa tgccgcatca ggcgctcttc
     3601 cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3661 tcactcaaag acggtgatgc ggttatccac agaatcaggg gataacgcca aaagaaacat
     3721 gtgagcaaaa ggccaggaac aggaagaagg ccgcgccgct ggcgtttttc cataggctcc
     3781 gcccccctga cgagcatcac aaaaacagat gctcaagtca gaggtggcga aacccgacag
     3841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3961 atagctcacg ctgttggtat ctcggcccgg tgtaggtcgt tcgctccaag ctgggctgtg
     4021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4081 ccaacccgga aagacacgta aaaacgccac tggcagcagc cattggtaac cgtttagagg
     4141 agttagtctt gaagttatgc gccggttaag gctaaactga aaggacagat ttggtggctg
     4201 tgctcctcca agccagttac cttggttcaa agagttgctg ctctgagaac cttcgaaaag
     4261 ccgccttgct gggcggtttt ttcgtttacg gacaagacta ccttcaggat cgttagatct
     4321 caagacaatc atgcatagtg agcatacaca ggagccgaac ttgcttacac tgttcatctt
     4381 ttttgtttta ttaatagctg catgtttctt ttgttttgct ccaccacgaa gaggatatga
     4441 cagaaatgag atcattccat acaaaataaa actatcaata aataaatatc gcctttacat
     4501 atactcatcg ggaaaggtaa gacaatacct tttattttta gttattttat cactgtacta
     4561 ctccatagcg gaaccattta agtctgagtt aat
//
LOCUS       DAFATA010000190         4527 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFATA010000190 DAFATA010000000
VERSION     DAFATA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.192"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            45..2075
                     /gene="malS"
                     /locus_tag="LGJ20_004650"
     CDS             45..2075
                     /gene="malS"
                     /locus_tag="LGJ20_004650"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBK2388633.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            2253..3506
                     /gene="avtA"
                     /locus_tag="LGJ20_004651"
     CDS             2253..3506
                     /gene="avtA"
                     /locus_tag="LGJ20_004651"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBK2388634.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3658..4131)
                     /locus_tag="LGJ20_004652"
     CDS             complement(3658..4131)
                     /locus_tag="LGJ20_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK2388635.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(4233..>4527)
                     /gene="yiaJ"
                     /locus_tag="LGJ20_004653"
     CDS             complement(4233..>4527)
                     /gene="yiaJ"
                     /locus_tag="LGJ20_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBK2388636.1"
                     /translation="LTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFN
                     IHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDQGAIT"
BASE COUNT         1134 a         1212 c         1155 g         1026 t
ORIGIN      
        1 gctggcaaac tgtctgaaat cgcagcaata aggactcatc caccatgaaa ctcgccgcct
       61 gttttctgac actccttcct ggcttcgccg ttgccgccag ctggacttct ccggggttcc
      121 ctgcctttag cgaacaggga acgggaacat ttgtcagcca cgcgcagttg cccaaaggta
      181 cgcgtccact cacgctaaat tttgaccaac agtgctggca gcctgcagat gcgataaaac
      241 tcaatcagat gctttccctg caaccttgta gcaacacgcc gcctcaatgg cgattgttca
      301 gagacggcaa atatacgctg caaatagaca cccgctccgg tacgccaaca ttgatgattt
      361 ccatccagaa cgccgccgaa ccggtagcaa acctggtccg tgaatgcccg aaatgggatg
      421 gattaccgct cacgctggat gtcagcgcca ctttcccgga aggagccgcc gtacgggatt
      481 attacagcca gcaaattgcg atagtgaaga acggtcaaat aacgttacaa cccgctgcta
      541 ccagcaacgg tttactcctg ctggaacggg cagaaactga cgcccctgcc cctttcgact
      601 ggcataacgc cacggtttac tttgtgctga cagatcgttt cgaaaacggc gatcccagta
      661 atgaccagag ttacggacgt cataaagacg gtatggcgga aattggcact tttcacggcg
      721 gcgatttacg cggcctgacc aacaaactgg attacctcca gcagttgggc gttaatgctt
      781 tatggataag cgccccattt gagcaaattc acggctgggt cggcggcggt acaaaaggcg
      841 atttcccgca ttatgcctac cacggttatt acacacagga ctggacgaat cttgatgcca
      901 atatgggcaa cgaagccgat ctacggacgc tggttgatag cgcacatcag cgcggtattc
      961 gtattctctt tgatgtcgtg atgaaccaca ccggctatgc cacgctggcg gatatgcagg
     1021 agtatcagtt tggcgcgtta tatctttctg gtgacgaggt gaaaaaaacg ctgggtgaac
     1081 gctggagcga ctggaaacct gccgccgggc aaacctggca tagctttaac gattacatta
     1141 atttcagcga caaaacaggc tgggataaat ggtggggaaa aaactggatc agaacggata
     1201 tcggcgatta cgacaatcct ggattcgacg atctcactat gtcgctggcc tttttgccgg
     1261 atatcaaaac cgaatcaact accgcttctg gtctgccggt gttctataaa aacaaaacgg
     1321 atactcacgc taaagtcatc gaaggcttta cacctcgcga ttacttaacc cactggttaa
     1381 gtcagtgggt ccgcgactat gggattgatg gttttcgggt cgataccgcc aaacatgttg
     1441 agttgcccgc ctggcagcaa ctgaaaaccg aagccagcgc cgcgcttcgc gaatggaaaa
     1501 aagctaaccc cgacaaagca ttagatgaca aacctttctg gatgaccggt gaagcctggg
     1561 gccacggcgt gatgcaaagt gactactatc gccacggctt cgatgcgatg atcaatttcg
     1621 attatcagga gcaggcggcg aaagcagtcg attgtctggc gcagatggat acgacctggc
     1681 agcaaatggc ggagaaattg cagggtttca acgtgttgag ctacctctcg tcgcatgata
     1741 cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt attactatta gcgccaggcg
     1801 cggtacaaat cttttatggc gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc
     1861 cgctgcaagg tacacgttcg gatatgaact ggcaggatgt tagcggtaaa tctgccgcca
     1921 acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg ccatcccgca attggcgcgg
     1981 gcaaacaaac gacactttcg ctgaagcagg gctacggctt tgttcgtgag catggcgacg
     2041 ataaagtgct ggtcatctgg gctgggcaac agtgactttt ccggcttccc gttcgtcagt
     2101 acctcgggaa gccgccaacc aggataaaat gtcagcccta atcagcgttg caggataaag
     2161 caccgctcac tctccaacag accgatttgc accccagcaa atgtagcgtt attgttacct
     2221 tccttgctac agagttcgac agatatcccg ctatgacatt ctcccttttt ggtgacaaat
     2281 ttacccgcca ctccggcatt acgctgctga tggaagatct gaacgacggt ttacgcacgc
     2341 ctggcgcgat tatgctcggc ggcggtaatc cggcgcagat cccggaaatg caggactact
     2401 tccagacgct actgaccgac atgctggaaa gtggcaaagc gactgatgca ctgtgcaatt
     2461 acgacggtcc acaggggaaa acggagctac tcacactgct tgccggaatg ctgcgcgaga
     2521 agttgggttg ggatatcgaa gcacagaata ttgcactaac aaacggcagc cagagcgcgt
     2581 ttttctactt atttaatctg tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc
     2641 tgttcccgct ggcaccggaa tacattggct acgctgacgc cggactggaa gaagatctgt
     2701 ttgtctctgc gcgtccgaat attgaactgc tgccggaagg ccagtttaaa taccacgtcg
     2761 attttgagca tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga
     2821 atccaacagg caatgtgatt actgacgaag agttgctgaa gcttgacgcg ctggcgaatc
     2881 aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct
     2941 tcagtgaagc gcgcccgcta tggaatccga atatcgtgct gtgcatgagt ctttccaagc
     3001 tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcactgcca
     3061 tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt
     3121 gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt
     3181 actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaagatcgct
     3241 gcctaattca taaaccagaa ggagccattt tcctctggct atggtttaag gatttgccca
     3301 ttacgaccga gcaactctac cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc
     3361 acaacttctt cccagggctg gataaaccgt ggccgcatac gcatcaatgt atgcgcatga
     3421 actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag
     3481 aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc gctacgctta tcaggcctac
     3541 atgatttctg caatatattg aatttgcaag aatttgtagg ccggataagg cgtttacgcc
     3601 gcatctggca taaacaaagc gcactattcc ggcgttgaga aacgccggaa aaacgtttca
     3661 aacaggctgc tgccgtagcc gatgccgctg cactttctcg acatccatgc actgcaacgc
     3721 ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc acagatcgca
     3781 cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc
     3841 cagcatacag cttttacagc caatacaacg tgattgttca acgaaaatat gcccatgttc
     3901 gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac actgatgaca
     3961 ggctactgcc gtggtccagc tgtggtcttt aatgacacga atacgggaaa taaactcgtc
     4021 tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag ccacttcaca
     4081 ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca tgatcactcc
     4141 tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat ccaacgaagg
     4201 aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgccctg gtcgtccctg
     4261 acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga
     4321 tttttctccc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc
     4381 acccgcccat gaatattaaa caccggaaca gcaatacagg agacgccgag ttcgttttct
     4441 tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc
     4501 agctcggtaa tggtattgcg ggttaac
//
LOCUS       DAFATA010000191         4524 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFATA010000191 DAFATA010000000
VERSION     DAFATA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.193"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..653
                     /gene="frlA"
                     /locus_tag="LGJ20_004654"
     CDS             <1..653
                     /gene="frlA"
                     /locus_tag="LGJ20_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBK2388637.1"
                     /translation="RALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWI
                     PALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPD
                     VSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPA
                     FGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            674..1696
                     /gene="frlB"
                     /locus_tag="LGJ20_004655"
     CDS             674..1696
                     /gene="frlB"
                     /locus_tag="LGJ20_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBK2388638.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            1746..2576
                     /gene="frlC"
                     /locus_tag="LGJ20_004656"
     CDS             1746..2576
                     /gene="frlC"
                     /locus_tag="LGJ20_004656"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBK2388639.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            2573..3358
                     /gene="frlD"
                     /locus_tag="LGJ20_004657"
     CDS             2573..3358
                     /gene="frlD"
                     /locus_tag="LGJ20_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBK2388640.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            3458..4189
                     /gene="phoB"
                     /locus_tag="LGJ20_004658"
     CDS             3458..4189
                     /gene="phoB"
                     /locus_tag="LGJ20_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBK2388641.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(4341..>4524)
                     /locus_tag="LGJ20_004659"
     CDS             complement(4341..>4524)
                     /locus_tag="LGJ20_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388642.1"
                     /translation="ALPYPVGAESKYEIPPLFYRLSGTFRQANPQSEHCAIRINPNRS
                     GEETVLRILRESIASI"
BASE COUNT         1024 a         1226 c         1245 g         1029 t
ORIGIN      
        1 cacgagcgct gattggttcc tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg
       61 tgatttccgg cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg atttccgacg
      121 ccctgacctg gatccccgca ctcggcagca ccgctgggat ctttgttgcc atcacggcga
      181 tgatcgtcat tcttggttcg ctttccagct gcgtgatgta ccagccgcgg ctggaatacg
      241 cgatggcgaa agacaacctg ttctttaaat gcttcggcca tgtgcatccg aaatacaaca
      301 cgccggatgt ctccatcatc ctgcaagggg cgctggggat cttcttcatc ttcgtttccg
      361 atctcaccag cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat accctcacct
      421 tcggctccat catctggtgt cgtaaacgcg acgattacaa accgctgtgg cgtactccgg
      481 ctttcgggct gatgaccacc ctcgccattg cgtcaagcct cattctggtc gcctcaacct
      541 ttgtctgggc accgattccc ggccttatct gcgccgtcat cgttattgct actggtctgc
      601 ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg taatgttatc
      661 tggagaaaat aaaatgttgg atattgataa aagcaccgtg gactttctgg tcaccgaaaa
      721 tatggttcag gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc acaccatcgt
      781 ggaagagatg gtgaagcgcg acattgatcg tatttatttc gttgcctgcg gatcgccact
      841 caacgcggcg caaacggcga aacatctggc ggatcgcttt tccgatcttc aggtctacgc
      901 catttccggc tgggagttct gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt
      961 gattggcgtt tctgactacg gtaaaacgga agaggtaatc aaagcgctgg agctgggccg
     1021 ggcctgcggc gcactcactg cggcgttcac caaacgcgcg gacagcccga ttacctcggc
     1081 ggcggaattt agcattgatt atcaggccga ctgtatctgg gaaattcacc tgctgctctg
     1141 ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat
     1201 caaaaacgat ctcaagcaat tgccgaatgc gctcggtcat ctggtacgca cctgggaaga
     1261 aaaaggccgc cagcttggtg aactggccag ccagtggccg atgatttata ccgttgctgc
     1321 gggtccgctg cgtccgctgg gttacaaaga aggcattgta acgctgatgg aatttacctg
     1381 gacgcacggc tgcgtgattg agagcggaga gttccgccat ggcccgctgg agattgtcga
     1441 accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg
     1501 cgccattaac tttgttaaac agcgtactga caacgtgatc gtcatcgatt acgccgaaat
     1561 ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa tggagtggct
     1621 ctgctactac ctgtctattt acaaagatca caacccggat gaacgccgct attacggtgg
     1681 tctggtggaa tattaatccc tctcccggcc cggcaacggg ccgaatttac gcaaggagtt
     1741 acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat
     1801 ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg
     1861 cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt
     1921 atcagatgcc gattatcggc tatacgccag aaaccaacgg ctatccgtat aacatgatgc
     1981 tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat caagctggcg atggatatgg
     2041 caaaagagat gaacgcgggt tatacgctga tttccgcggc ccacgcgggc tatctcacgc
     2101 cacctaatgt tatctgggga cggctggcag agaacctaag cgaactgtgt gagtacgcgg
     2161 aaaacatcgg catggatcta atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt
     2221 gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca
     2281 tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac
     2341 tgggcgataa attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt
     2401 acattcctgg agaaggcaaa atgccgctga gggaactgat gcgcgatatt atcgaccggg
     2461 gctatgaggg ttactgtacg gtggagctgg tgacgatgta tatgaacgag cccagactct
     2521 atgcccgcca ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac
     2581 cctggcgaca atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc
     2641 tggcggtaat gcggtcaatg tggcggtgta ctgcactcgc tacggcatac agccgggatg
     2701 cattacctgg gtgggtgacg atgactacgg cacaaagctg aagcaggatc tcgcccgcat
     2761 gggcgtcgat atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga
     2821 actgcacgac aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc
     2881 cctgagtgaa gaggattacg cctggctggc gcagtatgac attgtgcacg cggcaatctg
     2941 gggacatgcg gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga
     3001 cttctccgac aagtgggaca gctcgctctg gcagacactg gtgccgcatc tcgattttgc
     3061 ctttgcctcc gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc
     3121 ccgtggcgca ggcgtggtga ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg
     3181 cgcgcagttc tggcgtcagg ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg
     3241 agattcgttc attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc
     3301 gatggcgcag ggaacggcgt gcgcggcgaa aaccattcag taccacggtg cctggtaggt
     3361 ataacgttgg cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta
     3421 taatccgagt aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc
     3481 tcatcaactc ctctacgcta ccgtccgcca gcggttgctg gatgatatcg cgcagggggt
     3541 ttaccaggcc gggcaacaga tccctaccga aaacgagctt tgtacgcaat ataacgtcag
     3601 ccgcattact attcgcaaag ctatcagcga cttagtggca gacggcgtac tgatccgctg
     3661 gcagggcaaa ggcacctttg tacaaagcca gaaggtcgaa aacgccctgc tgaccgtcag
     3721 tggttttacc gattttggcg tctcacaagg caaggcgacg aaagagaaag tgatcgagca
     3781 ggaacgagtc agcgccgcgc cgttttgcga aaagctgaac atccctggca acagcgaagt
     3841 gttccatctt tgccgggtga tgtatctcga taaagagccg ctgtttattg atagttcctg
     3901 gatcccactg tcgcgttatc ctgactttga tgagatttac gttgaaggaa gctccactta
     3961 tcagttattt caggagcgtt tcgacacgcg agtggtcagc gacaaaaaga ccatcgatat
     4021 ctttgccgcc acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt
     4081 tcgcatcagc aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt
     4141 ctgccgcgcc aatcgcatca ccttaacgat tgataataaa agacattaac cgtaggccgg
     4201 ataagatgcg ccagcatcgc atccggcgat gctggcgcgt tgaattttac atcccgtacg
     4261 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcagta cggaatttag
     4321 cttgacggtg ctatccagca ttaaatactg gcaatactct cacgcaaaat ccgcagcacc
     4381 gtctcttcac cgctgcgatt cgggttaatg cgaatcgcac aatgttctga ttgtggattc
     4441 gcctggcgaa acgttccgga aaggcgataa aagagcggcg ggatttcata tttcgactct
     4501 gcacccaccg ggtaaggcaa ggca
//
LOCUS       DAFATA010000192         4489 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFATA010000192 DAFATA010000000
VERSION     DAFATA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.194"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..438
                     /gene="xanP"
                     /locus_tag="LGJ20_004660"
     CDS             <1..438
                     /gene="xanP"
                     /locus_tag="LGJ20_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBK2388643.1"
                     /translation="NSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVALMLIVL
                     GLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIALSLAVGL
                     GVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            559..2268
                     /locus_tag="LGJ20_004661"
     CDS             559..2268
                     /locus_tag="LGJ20_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBK2388644.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(2321..>4489)
                     /gene="yicI"
                     /locus_tag="LGJ20_004662"
     CDS             complement(2321..>4489)
                     /gene="yicI"
                     /locus_tag="LGJ20_004662"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBK2388645.1"
                     /translation="PLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTI
                     ENTERYAEFKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFE
                     RLDLGVGETVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLV
                     NHPQCVSFEVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWS
                     FGLWLTTSFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFP
                     DPEGMIRRLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGL
                     AIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAY
                     IYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSI
                     GLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDV
                     VRFFTQLKCRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMV
                     APVFTEAGDVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNN
                     DQRPDYVWHEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAK
                     NWTLCLRNVVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
BASE COUNT         1055 a         1232 c         1159 g         1043 t
ORIGIN      
        1 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
       61 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
      121 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
      181 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
      241 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
      301 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
      361 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
      421 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
      481 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
      541 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
      601 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
      661 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
      721 cgtttttccg cgccatctca tatcgtgctg gagaacgtca cgtttggccg tgatggccag
      781 cccgcgaccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcggca actgaccgaa
      841 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
      901 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
      961 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtagttcc gtggtcacca
     1021 aaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
     1081 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
     1141 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
     1201 aacgccgacg gcagctggca agtggaaaac ctgcgcatgg cggatatacg tctacaaagc
     1261 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
     1321 cgcctggaag tgatcgatgc tcgtttgcaa ggtccggact gggcggtgac cgacctcgat
     1381 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
     1441 tcgatgaacg ctagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
     1501 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
     1561 atggtcagaa cgtcagggaa ctggctgcgt gacgggaaaa cgttgatcct tgatgatgtg
     1621 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
     1681 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
     1741 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
     1801 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
     1861 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
     1921 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
     1981 accgccagtg tttcacaaac gccacaacgt cagacacata tcagcctgaa tggacgcggt
     2041 gtgccggtga atattttgca acagtgggga tggcctaaat taccgttgac tggcgacggc
     2101 aatattcagc ttaccgccag tggcgatatt caggccaatg tcccgttgaa acctacggtt
     2161 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgct
     2221 ggcatcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
     2281 ccgggtacga ctcagacgta cccggcattc catcaataga ttacaacgta attgtcagcg
     2341 cattcccttg aggcttcacc accagcccct gctcactttc agcctgcgaa ccgtcttgca
     2401 gaccatttac tttcacaaca ttgcgcaggc acagcgtcca gttcttcgcc tcgcccgcac
     2461 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
     2521 cagtagcggg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
     2581 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtaggg
     2641 tgttatcacg cacataaatg ggcagactca ggaagctgtg ctgctgttta tgccagcgac
     2701 taccgtcgag ctcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
     2761 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
     2821 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
     2881 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
     2941 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
     3001 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
     3061 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
     3121 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
     3181 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
     3241 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
     3301 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
     3361 actgaacatc agttgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
     3421 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
     3481 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
     3541 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
     3601 taatccagac gcagattttc agtcctttcg ctttcaggcg gcggatcatc ccttccgggt
     3661 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
     3721 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
     3781 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
     3841 acgcgggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
     3901 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
     3961 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
     4021 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
     4081 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
     4141 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
     4201 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
     4261 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
     4321 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
     4381 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
     4441 caccgacaat accttcctgt ggggagaaaa agcgcaacgt aaataaagg
//
LOCUS       DAFATA010000193         4448 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFATA010000193 DAFATA010000000
VERSION     DAFATA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.195"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..620)
                     /locus_tag="LGJ20_004663"
     CDS             complement(<1..620)
                     /locus_tag="LGJ20_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBK2388646.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
     gene            complement(831..2228)
                     /gene="ccp"
                     /locus_tag="LGJ20_004664"
     CDS             complement(831..2228)
                     /gene="ccp"
                     /locus_tag="LGJ20_004664"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBK2388647.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2633..4282
                     /locus_tag="LGJ20_004665"
     CDS             2633..4282
                     /locus_tag="LGJ20_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBK2388648.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4333..>4448)
                     /locus_tag="LGJ20_004666"
     CDS             complement(4333..>4448)
                     /locus_tag="LGJ20_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK2388649.1"
                     /translation="RALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         1129 a         1135 c         1051 g         1133 t
ORIGIN      
        1 ggcaccacgc cgatggtgtt ttcgtcacag gcttcaatca tgcgtttcgg gtccataaac
       61 aactgaccgg ggcgcatagg gatctcacgc agctccacat cccagtagcg ggcgaattta
      121 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgttgg tttgcctgca
      181 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      241 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggcgcagg cgcatgccac
      301 agatcggcaa ccatatttac gcaacgcagg tcgatggctg cggattgcgg atattcttct
      361 ttgtcgatcc agtttttatt gatcgacaaa tccatcaatt tatggacgtt ttcgtcgtcc
      421 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcatca
      481 ttgataatct ggaatgcgac atcatcgcgc atttcgtgca gcggaaatcg ttttgactcc
      541 gcgatagtag aaatggcctt tgcgccaaaa cgtgaatcga gtagttctga gcggaaatcc
      601 gttaacagct tctggtccat ttcgaactcc ttaaatttat ttgaaggcaa taaaaaagta
      661 ggatttatcc gcaatggaag caaggcatta caggcttaat ttaaataaca aaatcctaag
      721 cagaaaaacg actttttata atataaacat ttaacatgat aatattaaaa acagacttta
      781 ttatcgtaat aaaaatcgcc caaacattcc tgttcgggcg atcaaattaa ttattgttta
      841 tcctgcatat acggcgtgta caccccgttc agactgtgca ggaaagctac gatatcatcc
      901 acatcctcct gcggcagctc tttgcctacc tgatagcgca gcatcagttt caccgccccg
      961 tccagcgtcg gcacgtcacc gcgatggaag tacggtgccg ttaaagcaac gttacgtaaa
     1021 ccgggtactt tctgacgcaa tttatcacgc tcttctttag tcacattcat acgaccaata
     1081 tccgccgccg taatttcccc aaagttaaag tcttttttca gccccaacgg ttcaaaggaa
     1141 cgtccgccga gaataatacc accatgacaa gttgcacatt tattatcttt aaataattga
     1201 tagccttttt tctgttgcgc cgtcagcgca ttttcatctc cacgcaacca tttatcaaat
     1261 ggggaatccg gcgtaattaa tgttttctca aattcagcaa tggcatcagt aatattttcg
     1321 ccactgaaac cttgcggata aacttcgagg aactgcgctt taagctgcgg atctttttcc
     1381 agcttagcaa taatttcgtc ccaggatttc gacgccattt caatcgggtt caacggcggt
     1441 ccacctgcct gatcctgcaa tgttgccgca cgaccatccc agaactgctc aacgttaaat
     1501 actgagttaa ataccgtcgg cgcgttaatc ggcccaactg cgccaccaac accaatcgat
     1561 gtttttctgc catcgacgcc ccccgcattc aacgcatggc aatgagcgca tgaaatggtg
     1621 ctatcagccg ataaacgggg atcgtgatac agcgcaaagc ccaacgccac tttttgcgca
     1681 tcggtaggca gtttttgcgg gatgggctgc accggttcat tgcgatgctc cggagcagta
     1741 tcattgctgg cgtaatattc cgcgcgctgt tttgcaatcc aggccagtat ttccgcccgc
     1801 tcttcatcac tcaccttacc cgcccagtgt agcgcggtat aacgcgttgg tggcatagtt
     1861 tcatactgca tcacccattc aatcttattc agatcgcttt gcgaaacggg tttatcagcc
     1921 agcagtgccg cacgcacggc ctcgaggtta aaagatttat atccaagctt aatgtcgtaa
     1981 tccatcaact gtttcgcgcc aggaatataa taataggcgg gtaattctgc cgaaggtgtg
     2041 tggcaatagt cgcatccttt ttcgcggaga aagcctaaaa ccttattatt ttcactgaca
     2101 gcagatgcct gaacatcggc ctgtttactg cgtttattat cgtggtacca cacataacca
     2161 gataacccta aatagcaaat cgcgacgcca gccaggccga tcgcggtaat acgtgagacc
     2221 attttcatta ttcttccctc acggtggtga gttattgtga cccagaaaat tttccagaat
     2281 gctgcaaacg tctctgcgag acaaagccat cataaaaaac agggagatac gctttttgat
     2341 aggaatcaat caattacatg aatataaact atcaataaga tagcctgtgc ctattaccca
     2401 gatcaggaat ggcatatcat gattgcggcg agagcaggat tgaatgttct gaaaatgaaa
     2461 aacaacacga aatccgtatt tttataaatt ccatggatat tagtgcgtta tattttttga
     2521 caggttaata aacgtgatcc accgcacgct ttgtcgccca ccaggcggag cgaatgacta
     2581 cccttaaaga aaagcccgat aattagcgac gaatttcgga ggttggatcc ttatgctcaa
     2641 tcagaaaatt caaaacccta atccagacga actgatgatc gaagtcgatc tctgctatga
     2701 gctggacccg tatgaattaa aactggatga gatgatcgag gcagaaccgg aacccgagat
     2761 gattgaaggg ctgcccgcct ctgatgcgct gacgcctgcc gatcgctatc tcgaactgtt
     2821 cgagcatgtt cagtcggcga aaattttccc cgacagtaaa acctttcccg actgcgcacc
     2881 caaaatggac ccgctggata ttttaatccg ctaccgtaaa gtgcgccgtc atcgtgattt
     2941 tgacttgcgc aagtttgttg aaaatcactt ctggctgccg gaggtctact ccagcgagta
     3001 tgtatcggac ccgcaaaatt ccctgaaaga gcatatcgac cagctgtggc cggtgctaac
     3061 ccgcgaacca caggatcaca ttccgtggtc ttctctgctg gcgctaccgc agtcatatat
     3121 tgtcccgggc ggccgtttta gcgaaaccta ctattgggat tcctatttca ccatgctggg
     3181 gctggcggaa agtggtcggg aagatttgct gaaatgcatg gccgataact tcgcctggat
     3241 gatcgaaaac tatggtcaca tccccaacgg caaccgcacc tattatttga gccgatcgca
     3301 accaccggtt tttgcgctga tggtggagtt gtttgaagaa gatggtgtac gcggtgcgcg
     3361 ccgctatctc gaccacctta aaatggaata tgccttctgg atggacggtg cagaatcgtt
     3421 gatccctaat caggcctatc gccatgttgt gcggatgccg gacggatcgc tgctcaaccg
     3481 ttattgggac gatcgcgaca cgccgcgtga cgaatcctgg cttgaggacg ttgaaaccgc
     3541 gaaacattct ggtcgcccgc ccaacgaggt gtaccgcgat ttacgcgcgg gagcggcctc
     3601 aggttgggat tactcttccc gttggctgcg tgatactggt cgtctggcga gcattcgtac
     3661 cacccagttc atccccatcg atctgaatgc cttcctgttt aaactggaga gcgccatcgc
     3721 caacatctcg gcgctgaaag gcgagaaaga gacagaagcg ctgttccgcc agaaggccag
     3781 tgcccgtcgc gatgcggtaa accgttacct ctgggatgat gaaaacggca tctaccgcga
     3841 ttacgactgg cgacgcgaac aactggcgct gttttccgct gccgccattg tgccgctcta
     3901 tgtcggcatg gcgaaccatg aacaggccga tcgtctggca aacgccgtac gcagccggtt
     3961 actgacacct ggcgggattc tggcaagcga gtacgaaacc ggtgaacagt gggataaacc
     4021 caatggctgg gcaccgttac aatggatggc aattcaggga tttaaaatgt atggcgatga
     4081 ccttctgggt gatgaaatcg cgcgcagctg gctgaaaacg gtgaatcagt tctatctgga
     4141 acagcacaaa atgatcgaga aataccatat tgccgatggt gttccccgcg aaggcggcgg
     4201 tggcgagtat ccgttgcagg atgggtttgg ctggactaac ggtgtggtac gccgtttaat
     4261 tggtttgtac ggcgaaccat aatattttta cagccagccg ctaacttcct gctggctgta
     4321 aaattatcct cttcaggagg agatatttaa catcattgcc gcctgggtgc gatttttcac
     4381 ttccagacgg cgatacaggg attccagatg cgcttttacc gttccggtac tgatattcag
     4441 cgctctgc
//
LOCUS       DAFATA010000194         4413 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFATA010000194 DAFATA010000000
VERSION     DAFATA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.196"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..2545)
                     /gene="fdnG"
                     /locus_tag="LGJ20_004667"
     CDS             complement(<1..2545)
                     /gene="fdnG"
                     /locus_tag="LGJ20_004667"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1958..1960),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBK2388650.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPE"
     gene            2777..3658
                     /gene="yddG"
                     /locus_tag="LGJ20_004668"
     CDS             2777..3658
                     /gene="yddG"
                     /locus_tag="LGJ20_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBK2388651.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            3917..4207
                     /locus_tag="LGJ20_004669"
     CDS             3917..4207
                     /locus_tag="LGJ20_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2388652.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            4231..>4413
                     /locus_tag="LGJ20_004670"
     CDS             4231..>4413
                     /locus_tag="LGJ20_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBK2388653.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWH"
BASE COUNT         1077 a         1128 c         1045 g         1163 t
ORIGIN      
        1 gttccgggaa cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg
       61 gctgcatgat aaacggcccg gttggcgtac ccggtgcggc attgccgaag tccggaatat
      121 cgttacccgt ccacttgctg ccgttccact ggatcagcat ccgtttcgga tcccacggtt
      181 taccgttgat atccgccgaa gcacggttgt acagcacgcg acggttgagc ggccacgccc
      241 aggcccatcc cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct
      301 ggttgccctg ctctgtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt
      361 catcacgcag atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat
      421 ttgcgtcata gagatcttcc agcgcatagc cgttgttctc ttttgccact tcgtcagatt
      481 gcggttcgtg cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac
      541 caccttcggc ctggtacaac tcgcgcagat gatggtagat acccgccaga atttcgccgt
      601 cgttacgcgc ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg
      661 agttagcgat agaaccatcc tcttcagcaa agcaggtcga aggcagacgg aatacttcag
      721 tctgaataga cgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg
      781 tttcagtcac cagcggatcg ataaccacca tgtacttcag cttgctcagg cagctcacca
      841 ctttgttttt gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga
      901 ctttgccttc atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg
      961 gcagccagtc atagccccag ttgttctctt tctgcgcggc atcgccatag aaagatttca
     1021 tcaggctaac gaagaacttc ggatagttgc tccagtagtt cacctgatca gctagcgtcg
     1081 ctttcggcgt gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg
     1141 tcagataacc tggcaggctg gtagagagca ggcctaagtc agtcaagccc tgaatgttgg
     1201 agtgaccacg caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact
     1261 ggatcatcgc catagtacgg atgttctgcg cacccacagt gtgctgcgtc cagcccagcg
     1321 cgtacaggaa ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt
     1381 tcaggaagtc ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg
     1441 aaacgtgcgc tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt
     1501 tcgcatagcc gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt
     1561 cagcgtcgta gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc
     1621 tggcgttggt gtaatgctta acgtattcgg cgttgatttt gtcgttttcg atcaggtagc
     1681 gcaaaacgcc agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg
     1741 ccacagaagc ggtacgcgta aaacgaggat cgacaacgat caaggttgca tcgttgttgt
     1801 ttttcgcttc catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca
     1861 ttaccattac gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac
     1921 caaatgttgg agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta
     1981 ccgccagcat cccgagggag cgggcaaatt tctgtgtcag catcccggtt tcgttgctgg
     2041 caccggaggc acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt
     2101 tcttttcaat aaagttagcg tcacggtcag ctttcatcag ctttgcaata cgggagaatg
     2161 cttcttccca gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc
     2221 gcagacggtt ttcgctgttg acgtaatcca gcaaaccggc cccttttggg cacagcgcac
     2281 cacggcttac cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt
     2341 tcgcgccatc acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg
     2401 tgttacggat ctctttagcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct
     2461 tcggggcaaa gcccaatgcc gctaccgttg ttccagccat accgcccgcg cagattttaa
     2521 aaaattgtct gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga
     2581 ggaaactaac agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc
     2641 cacaaaataa tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac
     2701 tgcctgacag gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag
     2761 agacaaaaca gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt
     2821 cctgtggagc acgatggtag gattgattcg cggtgtcagc gaggggctcg gcccggtcgg
     2881 cggcgcagct gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc
     2941 gcgtattcgg cagattccga aaggctattt actcgccggg agtctgttat tcgtcagcta
     3001 tgaaatctgt ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt
     3061 gggtatggtg aactatctat ggcccagcct gacaattctc tttgccattc tgtttaatgg
     3121 tcagaaaacc aactggctga ttgtaccagg attattatta gccctggtcg gcgtctgctg
     3181 ggtgttaggc ggtgataatg ggttacatta tgatgaaatc atcaataata ttaccaccag
     3241 cccattgagt tatttcctgg cgtttattgg tgcgtttatc tgggcagcct attgcacagt
     3301 aacgaataaa tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc
     3361 aagtctgtgg gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgcccgt
     3421 catgattaaa ctcatctctg cggcatttac cttaggattt gcttatgctg catggaatgt
     3481 cggtatattg catggcaatg tcaccataat ggcggtaggc tcgtatttta cacctgtact
     3541 ttcctcagcg cttgcagccg tgctgctcag cgccccactg tcgttctcgt tctggcaagg
     3601 cgcgctaatg gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggttaaaa
     3661 taattgccgg gtctacggcc cggcagttaa tcagataaat tccgacaata ttaaaaaata
     3721 aatttcaaat accagtaatt caccttatat ctcatttcga cttcgatcac atatcgataa
     3781 ataatattat ttcatctatt tttctcattg tttttaatca ccattctatt ttaaaaacag
     3841 tatgtcattt ataaatgacg ccgtttaaaa ttcgtttaga aaatacgttt aacgataatt
     3901 aaaaggattc tgttaaatga aattaaaaat agttgcggtg gttgtaactg gtttgttagc
     3961 tgcgaacgta gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta
     4021 tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac
     4081 ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg
     4141 tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaagaa
     4201 tctctgattg agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat
     4261 cccaggatga aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac
     4321 actacaaaac tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt
     4381 gatcaactaa tatcatcaaa acatctttgg cat
//
LOCUS       DAFATA010000195         4247 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFATA010000195 DAFATA010000000
VERSION     DAFATA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.197"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(83..610)
                     /gene="mobB"
                     /locus_tag="LGJ20_004671"
     CDS             complement(83..610)
                     /gene="mobB"
                     /locus_tag="LGJ20_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBK2388654.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(592..1176)
                     /gene="mobA"
                     /locus_tag="LGJ20_004672"
     CDS             complement(592..1176)
                     /gene="mobA"
                     /locus_tag="LGJ20_004672"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBK2388655.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1246..1515
                     /locus_tag="LGJ20_004673"
     CDS             1246..1515
                     /locus_tag="LGJ20_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBK2388656.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1592..2578
                     /gene="srkA"
                     /locus_tag="LGJ20_004674"
     CDS             1592..2578
                     /gene="srkA"
                     /locus_tag="LGJ20_004674"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBK2388657.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2595..3221
                     /gene="dsbA"
                     /locus_tag="LGJ20_004675"
     CDS             2595..3221
                     /gene="dsbA"
                     /locus_tag="LGJ20_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBK2388658.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            3376..>4247
                     /locus_tag="LGJ20_004676"
     CDS             3376..>4247
                     /locus_tag="LGJ20_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HBK2388659.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIA"
BASE COUNT         1127 a          954 c         1011 g         1155 t
ORIGIN      
        1 tccgtcagga tggccttttt gcattggcgc agaaaaaaat gccggatgcg acgctggcgc
       61 gtcttatcca gcctactctt gcttatccgt tctgcttttg catccactcc accacaaaat
      121 cagccagccc ctcaacatca ttaatatcca gtaatgcgac atcaagatta agcggcacat
      181 cactggctac agcaataaca tgcctgtcta tcactaattc ttcaggtcga tgtccggctc
      241 catcgcgaaa cagcacaatc tttgcgatct cttcatgctt aaacccttcg accagaatca
      301 aatccagctt tgaggtatcc attcgacttg cgagaaactg tagatccagc tcttcttcgt
      361 ctggtgtttc cgtcatcaag gcccatcgtt gctggctggc aacgatggtt tgtgccgcgc
      421 cagccttgcg cagctcatag ctatctttgc ctggcttatc aacatccata tcatggtgcg
      481 tatgcttaat cagccctgga cggatccctc tggcgcataa tgccgggatc agttgtttca
      541 aaagcgtagt ttttccggta ccactccacg cggcaaaggc gagtaacggt atcatcgttt
      601 ttcctgccat cgggcaagct cctctggcgt attcacgtta ataaatgcat ctttattatc
      661 gctgaaatca accgcatgac cgccagccag acgcataaat gccattaccc ggcgttctcc
      721 tgcttgcaga tattccagta ataaaggctc aattgcgcgg tttaccagag caatagtcgg
      781 gtgatcgcgt tcaccgtcgt ggacccacac gacaggcgca tctttgcgct gatgattaag
      841 ccgggctgct aaatcctcgg gaatataagg agtatcgcac ggacaaaaca aaaaccactc
      901 gcctgcttcc tgctgcatta ctgaaagcat tcctgccaga gggcctgggt aatccgccag
      961 tgaatcttca atcactttca gaccgcttgc ctgatagatt tcctgatggc gattggcatt
     1021 aaccacgacg tgagagagct gcgtcataag cgcgtcagcg acatgttgcc ataatggttt
     1081 gccgtttaat tcaagcaatc ctttatctac gccgcccatt cgtctggctt taccgcctgc
     1141 cagcacaacg cctgttatcg tcgtcatcag attcaccgat atcgcctctt ttattgtggg
     1201 attgaccctg ctaacgtgtc tgtctcaaga gtaaggagca ttactatgaa atgtaaacgt
     1261 ctgaatgaag ttattgaact cctccagcca gcctggcaaa aagagccaga ccttaacctg
     1321 ttgcaatttt tgcagaaact ggcgaaagag tcaggttttg acggcgaact ggcggatttg
     1381 acggatgaca ttctgatcta tcacctgaaa atgcgtgatt ccgcgaaaga tgcggtgatc
     1441 ccgggtttgc agaaagatta tgaagaagac ttcaaaacgg cgctgttacg cgctcgtggc
     1501 gtaattaaag agtaaaagct tgtaagcggt gccaccaaaa tcatcgtgaa atgatatcct
     1561 tcatcattcg taatgttttc cggatgatgg gatgaacaac agcgctttta ctttccagac
     1621 actacacccg gataccatca tggatgctct atttgagcaa gggatccggg tggattccgg
     1681 tcttaccccg cttaacagct atgaaaaccg tgtctatcaa tttcaggacg aagatcgtcg
     1741 acgttttgtc gtcaaatttt atcgccctga acgttggaca gccgatcaaa tcctcgaaga
     1801 acatcaattt gcgttgcagt tggtaaatga tgaagttccg gtcgcagcac ctgtggcctt
     1861 taacggtcag actttattga atcatcaggg attttatttc gctgtttttc caagcgtcgg
     1921 tggtcgccag ttcgaagctg ataatatcga tcagatggaa gcggttgggc gttatttagg
     1981 gcgtatgcac cagacggggc gcaaacagct ttttatccat cgcccgacca tcggtttgaa
     2041 cgaatatctc attgagccac gcaagctgtt tgaggacgct acactgatac cttccgggtt
     2101 gaaagcggca ttcctgaaag cgacagatga gctgattgcc gccgttacag cacactggcg
     2161 ggaagatttc accgttctgc ggctacatgg agactgccac gccgggaata ttctctggcg
     2221 cgatggtcca atgtttgttg atctggatga tgcacgtaat ggtccagccg ttcaggattt
     2281 gtggatgttg ctcaatggcg ataaagccga gcagcggatg caactggaaa ctattattga
     2341 agcttatgaa gaatttagcg agttcgacac cgctgaaatc ggactgattg aacctttacg
     2401 cgccatgcgt ttggtttatt atcttgcctg gctaatgcgg cgttgggctg atcccgcgtt
     2461 cccgaaaaat ttcccgtggt taaccgggga agattactgg ctgcgacaga cggcgacttt
     2521 tatagaacag gcaaaagttc tacaagaacc ccctttgcaa ttaacaccta tgtattaatc
     2581 ggagagagta gatcatgaaa aagatttggc tggcgctggc tggtttagtt ttagcgttta
     2641 gcgcatcggc ggcgcagtat gaagatggta aacagtacac taccctggaa aaaccggtag
     2701 ctggcgcgcc gcaagtgctg gagtttttct ctttcttctg cccgcactgc tatcagtttg
     2761 aagaagttct gcatatttct gataatgtga agaaaaaact gccggaaggc gtgaagatga
     2821 ctaaatacca cgtcaacttc atgggtggtg acctgggcaa agatctgact caggcatggg
     2881 ctgtggcgat ggcgctgggc gtggaagaca aagtgactgt tccgctgttt gaaggcgtac
     2941 agaaaaccca gaccattcgt tctgcttctg atatccgcga tgtatttatc aacgcaggta
     3001 ttaaaggtga agagtacgac gcggcgtgga acagcttcgt ggtgaaatct ctggtcgctc
     3061 agcaggaaaa agctgcagct gacgtgcaat tgcgtggcgt tccggcgatg tttgttaacg
     3121 gtaaatatca gctgaatccg cagggtatgg ataccagcaa tatggatgtt tttgttcagc
     3181 agtatgctga tacagtgaaa tatctgtccg agaaaaaata attcaatgta aattacataa
     3241 agcccgtgaa tattcacggg ctttttttat tatttaataa atataaatac attctgataa
     3301 tgcatcctgc cgctggacat tatttcagca taagtggata ttcaaagttt tgctgtttta
     3361 tcagggaata tttatatgat acgtaagtca gctacaggtg ttattgttgc gttagccgta
     3421 atctggggtg gtggcacatg gtacacaggt acgcaaattc agcctggtgt cgaaaaattt
     3481 attaaagatt ttaacgatgc taaaaagaaa ggtgaacatg cctacgatat gacgttaagt
     3541 tatcaaaatt ttgacaaagg cttttttaat tcccgttttc aaatgcaaat gacattcgat
     3601 aacggtgcac ccgatctcaa tatcaagcca ggccagaaag ttgtatttga tgtggatgtt
     3661 gagcacggtc cgttgcccat cacaatgtta atgcatggta atgtcatccc agcactggca
     3721 gcggcaaaag tgaacttagt gaataatgaa ctgacacaac cgctatttat cgccgcgaaa
     3781 aataaatcgc ccgtggaagc gacattgcga ttcgcgtttg gtggctcatt ctctacgaca
     3841 ttagatgttg cccctgcaga gtatggaaag ttttcttttg gtgagggcca gtttactttt
     3901 aatggtgatg gtagttcatt gtctaacctg gatattgaag gcaaagtcga agatattgtt
     3961 ctgcaattat caccaatgaa caaagtaacg gcaaaaagtt ttaccattga ttctctggcg
     4021 cgattagaag aaaagaaatt tccggttggt gaaagcgagt cgaaatttaa tcagattaac
     4081 attatcaatc acggggaaga cgttgcccaa atcgatgctt tcgttgcaaa aaccatgctg
     4141 gatcgcgtta aagacaaaga ttatatcaat gtcaatctca cctacgaact tgataagtta
     4201 acaaaaggga atcagcaact cggtagtggt gagtggtcat tgattgc
//
LOCUS       DAFATA010000196         4220 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFATA010000196 DAFATA010000000
VERSION     DAFATA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.198"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..273
                     /locus_tag="LGJ20_004677"
     CDS             <1..273
                     /locus_tag="LGJ20_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HBK2388660.1"
                     /translation="TAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARIIQRT
                     PDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            270..1349
                     /locus_tag="LGJ20_004678"
     CDS             270..1349
                     /locus_tag="LGJ20_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HBK2388661.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTGAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            1349..1897
                     /locus_tag="LGJ20_004679"
     CDS             1349..1897
                     /locus_tag="LGJ20_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HBK2388662.1"
                     /translation="MRAIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPRARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            1897..2322
                     /locus_tag="LGJ20_004680"
     CDS             1897..2322
                     /locus_tag="LGJ20_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HBK2388663.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSIILWRRDGPVMISFDDLWSAITHGGQ"
     gene            2309..3367
                     /locus_tag="LGJ20_004681"
     CDS             2309..3367
                     /locus_tag="LGJ20_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBK2388664.1"
                     /translation="MADSEFQRPMLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            3358..3942
                     /locus_tag="LGJ20_004682"
     CDS             3358..3942
                     /locus_tag="LGJ20_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBK2388665.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINENFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWLYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            3946..>4220
                     /locus_tag="LGJ20_004683"
     CDS             3946..>4220
                     /locus_tag="LGJ20_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388666.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKARHDQLLTALRALLLSRKNPFGDIKSDGTVKT"
BASE COUNT          902 a         1094 c         1345 g          879 t
ORIGIN      
        1 acggcaattg ataaggagtt gtcccgtaca accagtgatg cgctgtttct ggcgctgcgc
       61 cgggtgaaag cagatctgaa tgcggatatc aacacgcgcc ttgaacagtc tgcacggatc
      121 attcagcgca cgccggatga ggttttaccc gcgctggtgc tggcggcgac ctggtttgat
      181 aacgcggcgc gtgacgcgga cattatccgg cgtaatgcca ttacgcatcc cggctttgtg
      241 ccggtgatcc ctctgaaggt gccagtgcaa tgaacgacaa tgtcacgcta cgggtaaatg
      301 gccgggagtg gaatggctgg acatcggtgc gcatcggtgc cggtattgaa cggctggcgc
      361 gggatttcag tgtggagatc actcgccagt ggccgggaga tgagggtatc accacgcttc
      421 agccgcgcat taaaaacggt tcaaaagtgg aagtgctgat tggtgatgag ctggtgatca
      481 ccggctgggt ggaggcgact cccgttcgtt acgatgcccg ttcggtcagc accggtattg
      541 ccggacgtag tctgacggct gacctgattg actgtgcagc cgaaccgaca cagtttaacg
      601 gacgctcgct ggtgcagatt gcgcaggcgc ttgctgcgcc tttcggcatt gaggtggtga
      661 acagcggtgc gccgtcgggt gttattcctg atgttcagcc tgatcacggt gaaacggtga
      721 ttgaggtaat caacaaaata ctcggtcagc agcaggcact ggcttacgac gacccgcacg
      781 gcaggctggt gattggcggt attggctcaa cgggggcaca tactgcgctg gtactcgggg
      841 aaaacatcct ttcctgcgat acggagaaga gtatccggga gcggttttct gtttaccagg
      901 tggcggggca gcgtgccgga aacgacgatg atttcggtga ggccaccacc accgcgctgc
      961 gggcccgcac agaggacgca tttattgccc gttaccgtcc gatgtatatc aggcagacag
     1021 ggcaggctac gggggcaggc tgtattgccc gtgcggactt tgaagcccga caacgggcgg
     1081 cgcggacgga tgaaaccacc tatgtggtgc agggctggcg acagggtaac ggtacgctgt
     1141 ggcagcccaa ccagcgggtg attgtctttg atccggtctg tggtttcgac aataccgaac
     1201 tgcttgtttc ggaagtcacg tttactcagg accagaacgg caccctgacg gaaatccgtg
     1261 tcggcccacc tgatgcttat ctgcctgaac ccgaagcccc cggcgcgcgg aaaaagaaaa
     1321 aagccagagt acaggaggac ccgttctgat gagggcgatt gaagccatgc agcgacaact
     1381 cctcggcctg attgggcggg ccgtggtgaa aagcatcagt gccgccacga aatgtcagac
     1441 cgtggatgtg tccctgattg ccggtgaacc caaagccggg gttgaacatc ttgaacccta
     1501 cggttttacc gcaagggcaa acagcggtgc ggaagcggtg gtgttgtttc cggatggcga
     1561 ccgttctcat gcggtggttg ttacggtgtc ggaccggcgc taccgcctga aagggctgca
     1621 gacgggtgag gtggctgtct atgacgatca ggggcagtcc gtgacgctga cccgggaggg
     1681 gatcgtggtg gacggtgcag gtaaaacgat cacgtttcgc aatgcgccca gagcacgttt
     1741 tgaaatggac ctggaagtga caggacaggt gaaagacctg tgcgactccg gcggcactac
     1801 catgtcagcg atgcggcttg cctataacgg gcatcgtcac agagagaacg gtcagggcag
     1861 taacaccgac aaaccggata aagcgatgga ggcatgatgg aactgtggct gacggtgaac
     1921 ggtaaacgca cctgcgccag cgcaccgctg gatccgctga cccgcgccgt ggtgatttcc
     1981 ctgtttacct ggcggcgggc ggagcctgat gacaacgccg acgtcccgat gggatggtgg
     2041 ggggatacct ggcctgcggt acagaatgac cgttacggct cccgactgtg gctgcttcag
     2101 cgcagcaaac tgaccaatca gctggtgcag acagtaaggg ggtatatccg cgaatgcctg
     2161 caatggatga ttgatgacgg cgtggtgtcc cgtattgatc tggatatccg ccgcaccggg
     2221 attaatgagc tgggtaacag tatcatcctc tggcgtcgtg acggaccggt aatgatttct
     2281 tttgatgatc tgtggagtgc gataacgcat ggcggacagt gaatttcagc gcccgatgct
     2341 ggcagaaaat atcagtatgc tccgtaacga tttattcgcc aggctggacg tcagcgacac
     2401 gctccggcgc atggatgaag acgtgcgggc aaaggtgtat gcggcggcgc tgcatacggt
     2461 ttacggttac atcgattatc tggcaatgaa catgctgcct gacctgtgcg atgagtcctg
     2521 gctggcgcga catgctgcga tgaaacggtg tccgcgcaag ggggccacgg ctgccagcgg
     2581 gtatatgcgc tgggaaggtg tcagcgatgg cctgaaggtg accgccggga gcgtgattca
     2641 gcgcgatgac ctggttcagt acacggcaac tgctgatgca accagctccg gtggtgtcct
     2701 gcgcgtgccg atcgcctgct caagtgcagg cgcggtcggt aacgctgacg acggtacggc
     2761 attaatcctg gtcacgccgg tgaatggtct gccgtcttcc ggtgttgcag ataccctgac
     2821 tggcggattc gatactgaag atctggaaac gtggcgcgcc cgcgtcattg agcggtatta
     2881 ctggacgcct cagggcgggg ctgacgggga ctatgttgtc tgggctaaag aagtgcccgg
     2941 cattacccgc gcatggacat accgtcactg gatgggaacg ggaactgtcg gtgtgatgat
     3001 tgccagcagt gacctgatta atcccattcc ggaagaatca acggaaacgg cggcaagaca
     3061 acatatcggg ccactggccc cggtggcagg ctctgatttg tatgtgttca ggccggtggc
     3121 acatacggtg gattttcata tccgtgtgac gccggacaca ccggaaatac gggctgccat
     3181 caccgcggag ttgcgttcgt tcctgctgcg tgatggttat ccgcagggag aactgaaggt
     3241 atcgcgtatc agtgaggcga tttccggtgc gaacggggaa tacagccatc agttgcttgc
     3301 accggcagac aatatctcca ttgcaaaaaa tgaactggcg gtactgggga cgatttcatg
     3361 gacgtgacaa acgatgatta catccgtctg ttgtcggcac tgttgccccc cggtccggcg
     3421 tggtcagcca gcgatccggc gattgccggt gcggcaccgt cattaacccg cgttcatcag
     3481 cgtgcggatg ccctgatgcg ggagctggat ccgcgcacca ccactgaact gataaaccgc
     3541 tgggagcgtc tgtgcggtct gccggatgaa tgtattcccg cagggacaca gacccttcgc
     3601 cagcgtcagc aacggctgga tgcgaaggtt aacctggcgg gcggcatcaa cgagaatttt
     3661 tatcttgcac agcttgctgc cctgggcaga ccagacgcta ccatcacgcg atacgacaaa
     3721 agcacgttca cctgctcatc ggcctgtact gatgcggtga atgcgccgga atggctgtat
     3781 tactggcagg tcaacatgcc agccgccaca aacaccacct ggatgacatg tggcgatccc
     3841 tgtgattccg cgctgcgtat ctggggtgac accgttgtcg agtgtgtgct taacaaactc
     3901 tgcccgtcgc atacctacgt aatttttaaa tatccggagt aatccatgca tcgtatagac
     3961 acgaaaaccg cgcagaagga taaattcggc gcgggtaaga acggttttac ccgtggtaac
     4021 ccccagactg gcacgcctgc caccgatctg gatgatgact actttgacat gttgcaggaa
     4081 gaactttgta gcgttgttga ggcatccggt gccagtctgg agaaggcgcg gcacgaccag
     4141 ctgcttaccg cgcttcgtgc gctgctgtta agccgcaaga atccgtttgg cgatatcaaa
     4201 tcggatggca cggtgaaaac
//
LOCUS       DAFATA010000197         4180 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFATA010000197 DAFATA010000000
VERSION     DAFATA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.199"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            403..1653
                     /gene="frc"
                     /locus_tag="LGJ20_004684"
     CDS             403..1653
                     /gene="frc"
                     /locus_tag="LGJ20_004684"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBK2388667.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1707..3401
                     /gene="oxc"
                     /locus_tag="LGJ20_004685"
     CDS             1707..3401
                     /gene="oxc"
                     /locus_tag="LGJ20_004685"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBK2388668.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3471..>4180
                     /gene="yfdV"
                     /locus_tag="LGJ20_004686"
     CDS             3471..>4180
                     /gene="yfdV"
                     /locus_tag="LGJ20_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBK2388669.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFL"
BASE COUNT         1124 a          890 c          975 g         1191 t
ORIGIN      
        1 ctataaacac gcattttatc ttcctcaaag taaatcccaa taatcccgtg tcatcacaaa
       61 tcatttatta tgttatgttt tatggtacgc tttaaaaagc gtaatcatac ccttacttgt
      121 aaatcaccgg cgcttcttac agttgtaaga ataacatcac aaatgtcgcg actctctctg
      181 attaccacga atcaatattt atcatattct tcttttcgtc ctcactactt accatgcaga
      241 aaggaattgg aagtggcgat gaaatgattt tcagcacttg atacaggatt ttaaaaatat
      301 cgagtgcagc tacgacgata ttttgcgggt tatttataga agatattacg ccttaaataa
      361 acgctaaatt aacacatatt taattaacta ataggtattg ctatgtcaac tccacttcaa
      421 ggaattaaag ttctcgattt caccggtgtg caatctggcc catcttgtac tcaaatgctg
      481 gcctggtttg gcgctgacgt tattaaaatt gaacgtcccg gcgttggtga cgtaacgcgt
      541 caccagctgc gagatattcc tgatatcgat gcgctttact tcaccatgct taacagtaac
      601 aaacgttcta ttgagttaaa taccaaaaca gcggaaggca aagaggtaat ggaaaagctg
      661 atccgcgaag ctgatatctt agtcgagaac tttcatccag gggccattga tcacatgggc
      721 ttcacctggg agcatattca agaaatcaat ccacgtctga tttttggttc gatcaaaggg
      781 tttgatgagt gttcgcctta tgtgaatgta aaagcctatg aaaacgttgc tcaggcagcg
      841 ggtggcgcgg catccactac gggtttttgg gatggtccgc cgctggtaag cgctgcagcg
      901 ttgggtgaca gcaacaccgg aatgcatttg ctgatcggtt tacttgctgc tttgctgcat
      961 cgcgaaaaaa cggggcgtgg gcaacgagtc accatgtcaa tgcaggatgc cgtattgaac
     1021 ctttgccgcg tgaaattacg tgaccagcag cgtctcgata aattgggtta tctggaagaa
     1081 tacccgcagt atccgaatgg tacatttggt gatgcagttc cccgcggtgg taatgcaggt
     1141 ggtggcggtc agcctggctg gatcctgaaa tgtaaaggct gggaaaccga tcctaacgcc
     1201 tatatttatt tcactattca ggagcaaaac tgggaaaaca cctgtaaagc catcggcaaa
     1261 ccagaatgga ttaccgatcc ggcatacagt acagcccatg cacgacagcc acatattttc
     1321 gatatttttg ctgaaatcga aaaatacact gtcactattg ataaacatga agcggtggcc
     1381 tatttgactc agtttgatat tccttgtgca ccggttttaa gtatgaaaga aatttcactt
     1441 gatccctctt tgcgccaaag tggcagtgtt gttgaagtgg aacaaccgtt gcgtggaaaa
     1501 tatctgaccg ttggttgtcc aatgaaattc tctgccttta cgccggatat taaagctgcg
     1561 ccgctattag gtgaacatac cgctgctgta ttgcaggagc taggttatag cgacgatgaa
     1621 attgctgcaa tgaagcaaaa ccacgccatc tgatattaac gggggcttct ggctcccaat
     1681 ttataaaaat ttcgaggtta ttaatcatgt cagatcaact tcaaatgaca gatggtatgc
     1741 atatcatcgt tgaagcatta aaacagaata atattgacac tatttatggt gttgtaggta
     1801 ttcctgtgac ggatatggca cgccatgccc aggcggaagg cattcgttat attggttttc
     1861 gtcatgagca gtcggcaggc tatgccgctg cggcaagcgg ttttcttacc caaaaaccgg
     1921 ggatctgcct gacagtttct gcgccaggat tcctcaatgg tttgaccgca ttggccaacg
     1981 caacggtaaa tggttttccg atgatcatga ttagcggctc cagcgaccgc gcgatcgtcg
     2041 acctacagca aggtgattat gaagagctgg accaaatgaa tgcggcaaaa ccgtatgcca
     2101 aagcagcatt tcgcgttaat cagccgcagg atcttggcat tgcattggca cgcgctatcc
     2161 gggtctctgt atcgggtcgc cctggcggag tttatcttga tttgccagca aatgtcctgg
     2221 ccgcgacgat ggaaaaagac gaagcgttaa ccacgattgt taaagttgaa aatccatcgc
     2281 cagcattatt gccatgcccg aagtcagtca ctagcgcaat ttcgctttta gcaaaagctg
     2341 aacggccatt aattatcctt ggcaaaggcg cggcgtattc acaagctgat gaacagcttc
     2401 gtgaatttat tgaaagtact cagattccat tcctgccaat gtctatggcg aaagggatcc
     2461 ttgaagatac gcatccactt tctgcggcag ctgcgcgttc gtttgccctg gcaaatgctg
     2521 acgttgtcat gcttgttggt gcacgactga attggttatt ggcacacggt aaaaaaggat
     2581 gggcggcaga tacacagttt attcaactgg atattgaacc gcaggaaatt gacagcaacc
     2641 gccccattgc tgtgccagtc gttggtgata ttgcatccag tatgcaaggt atgctggcag
     2701 aactgaaaca aaacacattt acgactccac tggtatggcg cgatatttta aatatccaca
     2761 agcagcaaaa tgcacaaaaa atgcatgaaa aattaagtac agatactcaa ccattaaatt
     2821 actttaatgc attaagtgct gtgcgcgacg tattgcgcga gaaccaggat atttatttag
     2881 ttaatgaagg tgcaaatacc ctggataatg cacgaaatat tattgatatg tataaaccac
     2941 gtcgtcgtct ggattgtggt acctggggtg tcatgggcat cggtatgggc tatgccatcg
     3001 gtgctagcgt gacttctggt tctccggttg tcgccattga aggtgatagt gcttttggtt
     3061 tcagtgggat ggaaattgaa acgatttgtc gatataacct gccggtgacg atcgttattt
     3121 ttaataatgg cggcatctac agaggagacg gtgttgatct cagtggcgct ggtgcaccat
     3181 caccaacgga tctgttgcac catgcaaggt atgacaaatt aatggatgcg tttcgtggcg
     3241 ttggctataa cgtcaccacg acagatgaac ttcgtcatgc tttaaccacc ggtattcagt
     3301 cgcgcaaacc gaccattatt aatgtggtca tcgaccctgc agcaggaact gaaagtggcc
     3361 atattaccaa acttaaccca aaacaagtcg ctggtaatta aatactgaca tcgtctcctc
     3421 ttgccaactc attatgcgat ggtgagggga cccttatcgg aaatactatt atgctaacat
     3481 tttttattgg cgatttattg cctattatcg taatcatgct gttgggttat tttagcggca
     3541 gacgagaaac attttcagaa gatcaagctc gggcatttaa taaactggta ttaaactacg
     3601 cgcttcctgc ggctctattt gtatctatta ctcgggcaaa cagggaaatg atttttgcgg
     3661 acactcgtct gacccttgta tcacttgtgg ttattgtcgg atgtttcttt ttctcctggt
     3721 tcggttgcta caaatttttt aaacgtaccc atgcagaagc agctgtatgt gcattaattg
     3781 caggttcacc taccattgga ttcctggggt ttgcagttct cgatcctatt tatggtgatt
     3841 ccgtatcaac aggtttagtg gtagcaatta tttctattat tgttaacgca attactattc
     3901 ctattggtct gtatttgctg aatccttcct caggagcgga tggtaagaag aatagtaatc
     3961 tgagcgcatt aatttctgcg gcaaaggagc cagttgtatg ggcacctgtt ctggcaacga
     4021 tcctggtgtt ggttggggta aaaattccgg cagcatggga cccaaccttt aatctgattg
     4081 cgaaggctaa ctcaggggta gcggtattcg ctgcggggtt gactctggct gcacataaat
     4141 tcgagttcag tgccgaaatt gcttataaca ccttcctgaa
//
LOCUS       DAFATA010000198         4177 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFATA010000198 DAFATA010000000
VERSION     DAFATA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.200"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..448)
                     /gene="fimE"
                     /locus_tag="LGJ20_004687"
     CDS             complement(<1..448)
                     /gene="fimE"
                     /locus_tag="LGJ20_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBK2388670.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAER"
     gene            complement(926..1528)
                     /gene="fimB"
                     /locus_tag="LGJ20_004688"
     CDS             complement(926..1528)
                     /gene="fimB"
                     /locus_tag="LGJ20_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HBK2388671.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2984..3700
                     /gene="nanC"
                     /locus_tag="LGJ20_004689"
     CDS             2984..3700
                     /gene="nanC"
                     /locus_tag="LGJ20_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBK2388672.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            3720..>4177
                     /gene="nanM"
                     /locus_tag="LGJ20_004690"
     CDS             3720..>4177
                     /gene="nanM"
                     /locus_tag="LGJ20_004690"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBK2388673.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNG"
BASE COUNT         1330 a          850 c          812 g         1185 t
ORIGIN      
        1 cacgctccgc cagttcataa ccgcaagcat gccttaacat atgaggatga gtctgcgtta
       61 cggttccagc ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc
      121 gactcccgcg gcgagaaata aatatagcgt cagtccggtc agcgcctttc cagttagcac
      181 gttcctgggt ccatcgttcc acggcttcac gctcatcaaa acgtaacggg tgaacggtag
      241 aaaatccgtt cttcagtcgg cgaatattta ttctaccttc attaaggtca aggtcctgat
      301 aatgcagatc aagcagttca ctaatacgca tcccatgccg atatgccaac agaataagac
      361 aataatctct ggctcccgtt gccccgtaac aaaccgcctg catcatggcc tgaacttctt
      421 taccggtaag ataacgacgt ttactcacaa tagtagtact cctaactgag atatatttga
      481 atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa aaagataacc
      541 acacacggag tgatgtggct atcagtcaat tacattaaat atcatacaaa aataaaatat
      601 cactgatgtg acaacatcta ttttcattga tttgattcaa tataacaata gattgcagtg
      661 gattaacttc cagttgctta aaaaataaag aaaaaagtgc ggaaacagga gaatttaagt
      721 gatgtgtaaa tttatgaatg attttgttta tttattttga agaacataaa ccattgtatt
      781 ttgtatggat atggttaact taatgtttat tatgaaaatt aataatcaca cttacaataa
      841 catcaattaa taaatggtta atcatttttg atatatcgta agaataatgt agtttttaac
      901 accatccctg gtatctcaac tatctctata aaacagcgtg acgctgtcgt cctctggctc
      961 tatcccagat gccgtaaaaa cgccctgcat tgctggcggt ataccagaca gtatgacgaa
     1021 tattgcgatg cccaagataa tcctggataa gtcgcgtatc tattcccata ttcgccaaag
     1081 caaaaccaca cgaatggcgt aacatgtgcg gatgaatctc cagtgacaac ccggcattac
     1141 caccggaagt cgagataata tggtaaaact gttgccgaga aagcggattc cccttacgtg
     1201 ataaaaatac ccactcgctc tcagcatgcg ggtacgaagt acggatactc aaccagtttt
     1261 ttaaagcctg aacttcttta ttcaatagcg ggtgcgttgt tgaaaagcct ttttttaatc
     1321 gatggatata tatacacttt gccttaagat caatatccga aatcctcaat cgacaaattt
     1381 cactcgcccg gaaaccatga ataaaacaaa gcaaagtcag acaataatta cgtgctgcat
     1441 gaggcccggt atttgctgct ttaaggagtg attcgatttc actatgggtc aggaagttcc
     1501 tttttttgtt atcagcctta ttcttcatcg tttttccctt ataattacag acgcgcacta
     1561 gctgtgctgg gttagcaata atccaacatt tttatcctga ttatgtttat aaaagcgaac
     1621 gtttgcttaa taactaacgt aagtggacca gttcttctga gtgaacttaa atggagtagc
     1681 aacagttaat tataataacg ttgccataca aacgccatgc tcttgcatgc tatgtacctt
     1741 tatatattta tcaatcggat acgaatctaa taataaccct catcattaat gcaactaatc
     1801 ttatctatat atcatgtgat atgttctgta acaagtaaaa tcaacataaa aaattcagca
     1861 aaatttaata ttcgttcaac tagccgtctc acatatgaca ttattagtca actccatttg
     1921 ccacaaaatg gctatttcat ggagcgcagt aatttcttag tggctattat catgctaatt
     1981 cttaggcccc tagcgattat tcctgctgat tcgtcagctt acgccatgag attgttacat
     2041 tcctttttat cctggagata tgggggaacg caggagatga ttttttcatc tacattcgtt
     2101 ggtaaatata gctttggtca ctacgactta aaccttcctc aatcacagcc attcgccggg
     2161 cgatacggca aacaaactaa gtgaaacatc atcctgaaaa caccaacatc aacaagcctc
     2221 tccagatcga cttcagaagt gaccagttac aagccacaaa ccaggaaaca tatttcatga
     2281 tgagaattat gctcagcctg atggcgggta aacactgatt ttcaccaagc tcactgagtt
     2341 agcttgcaaa gcttcccttg tttagactct aaataaatta tttataaaac aaatagttaa
     2401 acaaagatct ttagtttttg aagaccaccg aaagtgttcg tctggcttca catggcatct
     2461 tcctcttaga aaaagatcga catattttgt gacacgaatt gcaaatctgg ttttgttgta
     2521 tggattgcgt gatttttgat ctggtataac aggtataaag gtgcaccaag atagtcaatg
     2581 agacagggca tctcgcaatc tatggcaaac atcacttcag ttctttctca tcgggtgatg
     2641 aaaacgcact tcagtctgaa aggaatatga aaatgagatc aacagacatt cgattttatg
     2701 actctgggta aaatggattg agtaagtgat atagcttacg aacattcaaa tcaattaaac
     2761 atcagaagag attttatact caggtattta atctggatct ctgtttattt aaataatgtg
     2821 aaaagagatt tttcacagga gaccttatac aaaaaaatat aaaatacagc taccggttgc
     2881 caaagacact ataagcctgg caaaaaaata ttacacaaca taaatgctaa ttgtttatgc
     2941 gggctttgta ttgctttctg tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat
     3001 actttctggc gtattattac tgtgcttttc gtccccatta atttctcagg ctgcgacact
     3061 ggacgtacgt ggtggatatc gtagtggaag ccacgcctat gagactcgac tcaaagtcag
     3121 tgagggatgg caaaatggat ggtgggcaag catggaaagt aatacctgga ataccattca
     3181 tgataataaa aaggaaaatg ccgcactcaa tgatgttcag gttgaagtta attacgcgat
     3241 taaacttgat gatcaatgga cggtgcgccc gggaatgtta acgcatttta gcagcaacgg
     3301 cacacgctac ggaccctacg taaaactgtc ctgggacgcg acaaaagatc ttaattttgg
     3361 cattcgctat cgttacgact ggaaagctta ccgacaacaa gacttatccg gtgatatgtc
     3421 tcgtgataac gttcatcgtt gggatggata tgtcacttac catattaata gtgatttcac
     3481 cttcgcatgg caaacgacgc tatacagcaa acagaacgat tatcgctatg caaaccataa
     3541 gaaatgggcg acggaaaatg catttgttct acaataccat atgacgcccg atattacgcc
     3601 atacatagaa tatgactacc tggaccgtca gggtgtttac aacggcagag ataatttatc
     3661 ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag tagacaggag acagtcacaa
     3721 tgaataaaac aataacggcg cttgctatca tgatggcttc atttgccgca aacgcgtctg
     3781 tattacctga aactcctgtg ccatttaaaa gtggtaccgg agcaattgat aacgacactg
     3841 tctacattgg tttaggtagc gcaggtacgg catggtacaa gctggataca caggccaaag
     3901 ataaaaaatg gacagcgtta gctgcattcc ctggtggacc aagagatcaa gcaacctcgg
     3961 catttattga tggcaatctg tatgtgtttg gcggcattgg caaaaatagc aagggcttga
     4021 ctcaggtatt taatgacgta cacaaataca accccaaaac caatagttgg gttaaattga
     4081 tgtcgcatgc accgatgggc atggcgggcc atgtgacttt tgtacacaac ggcaaggctt
     4141 atgttactgg cggtgttaac cagaatatct tcaatgg
//
LOCUS       DAFATA010000199         4080 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFATA010000199 DAFATA010000000
VERSION     DAFATA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.201"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="LGJ20_004691"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="LGJ20_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBK2388674.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="LGJ20_004692"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="LGJ20_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBK2388675.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>4080)
                     /gene="mdtF"
                     /locus_tag="LGJ20_004693"
     CDS             complement(2659..>4080)
                     /gene="mdtF"
                     /locus_tag="LGJ20_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HBK2388676.1"
                     /translation="EDQGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVF
                     TVGGFGFSGQGQNNGLAFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNL
                     PAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPM
                     FKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPD
                     NINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMK
                     FMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFS
                     VMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGK
                     TPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATV
                     LAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1104 a          912 c          915 g         1149 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ctgaacgttt tacaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgcagtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
//
LOCUS       DAFATA010000200         4062 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFATA010000200 DAFATA010000000
VERSION     DAFATA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.202"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..659)
                     /locus_tag="LGJ20_004694"
     CDS             complement(<1..659)
                     /locus_tag="LGJ20_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK2388677.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     T"
     gene            complement(678..1139)
                     /gene="tsaE"
                     /locus_tag="LGJ20_004695"
     CDS             complement(678..1139)
                     /gene="tsaE"
                     /locus_tag="LGJ20_004695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK2388678.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(1111..2658)
                     /gene="nnr"
                     /locus_tag="LGJ20_004696"
     CDS             complement(1111..2658)
                     /gene="nnr"
                     /locus_tag="LGJ20_004696"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBK2388679.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIER
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            2657..3796
                     /gene="queG"
                     /locus_tag="LGJ20_004697"
     CDS             2657..3796
                     /gene="queG"
                     /locus_tag="LGJ20_004697"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK2388680.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(3779..3832)
                     /locus_tag="LGJ20_004698"
     CDS             complement(3779..3832)
                     /locus_tag="LGJ20_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388681.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          968 a         1139 c         1038 g          917 t
ORIGIN      
        1 atggtgacat ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg
       61 tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc
      121 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
      181 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc
      241 gcaaccacgg gcggcggagg aggaggtggc ggcgcatcgg cgttaatcgt aaagacgaca
      301 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg
      361 accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga
      421 ttattgccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
      481 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
      541 gttatccgcg cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc
      601 accggcgtgc acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc
      661 ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa
      721 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
      781 gggtcaggaa gaacaccagt accttgttgt ggccactcca ccaggcagat ggcatcgttg
      841 gcaaaataat cgcgaatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
      901 tcaaagtgat aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg
      961 cttttgacat taccctgatg acccagagct tgtaaaaagc cccggctaaa ggtggtttta
     1021 cctgcgccta aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc
     1081 cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg
     1141 gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
     1201 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgcagcac cgtgcgcgac
     1261 acagcccgca caggcggcat catacggcga cagtttttgc ccaagcaatg cgccaataat
     1321 accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat
     1381 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
     1441 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcacgttcaa tttcagcgac
     1501 ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg
     1561 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
     1621 aaaattctca actttttgca gtgctttttt cccccactct tgctggccca gaccgggacc
     1681 aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatcg tcagttcatg
     1741 caccatcaat tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc gggtcagtac
     1801 tcggactaaa ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc
     1861 cgtgccgtga tcgccaccga taattaccag ccgcccgtga tcgcctttat gcgaagtcgg
     1921 gcgacgcggt tttagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc
     1981 ctgacctgcc agccaactat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc
     2041 ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc
     2101 gttgatcacc gcgcctggcg tagcgccagt ttcagccagc aggccggaag ggatatcaac
     2161 cgccacaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
     2221 gggcgcttgc cgcaaaccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg
     2281 ccagacaata ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag
     2341 cgcggcctct tccggcaacg gtttgtcgct ctcctgggcc aacaacgtga cctcaatgcc
     2401 gaccgctttg gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca
     2461 cagcaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc
     2521 gccagcgcga agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc
     2581 tccgcggcgg atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt
     2641 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
     2701 acagtgggga ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
     2761 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
     2821 ggcacgtcac ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt
     2881 gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
     2941 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
     3001 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
     3061 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
     3121 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttctttct tctttttagg
     3181 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggtaa
     3241 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
     3301 tgctcgccgc tgtatctctt atctcaccat cgaacttgaa ggggcgatcc cggaagagtt
     3361 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg
     3421 gaatcgctat tcacaactca ccacagaaga ggatttcagc ccgcgtaagc cgctacacgc
     3481 accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg
     3541 atcggcgatt cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg
     3601 caatgcccct tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc
     3661 acttctcgat gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg
     3721 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt
     3781 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
     3841 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta
     3901 tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta
     3961 cagcctgagg ctgtggataa ctctgtttac aagagtttgt cagagacaac gaaaaccagc
     4021 aataacgcat cgcatcgctg tggatatttt attgagagaa ga
//
LOCUS       DAFATA010000201         4045 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFATA010000201 DAFATA010000000
VERSION     DAFATA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.203"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..132)
                     /locus_tag="LGJ20_004699"
     CDS             complement(<1..132)
                     /locus_tag="LGJ20_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388682.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     "
     gene            complement(212..2845)
                     /gene="yebT"
                     /locus_tag="LGJ20_004700"
     CDS             complement(212..2845)
                     /gene="yebT"
                     /locus_tag="LGJ20_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBK2388683.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(2814..>4045)
                     /gene="yebS"
                     /locus_tag="LGJ20_004701"
     CDS             complement(2814..>4045)
                     /gene="yebS"
                     /locus_tag="LGJ20_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBK2388684.1"
                     /translation="IGEELPRGDYQRCPQCDMLFSLPEINSHQSAYCPRCQAKIRDGR
                     DWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANVMQGIWQMTKQGDTITGAM
                     VFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLKEWVMLDIYLVGIGVASIK
                     VQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFYPQRPATRRDEKLRVCLGC
                     HFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVLLLPANLLPISIIYLNGGR
                     QEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFTLLLSIHFKCQQGLRTRIL
                     LLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAAFYFGAAVILTILAVEWLD
                     SRLLWDAHESGNARFDD"
BASE COUNT         1021 a         1122 c         1007 g          895 t
ORIGIN      
        1 attaacgcga atgctgcggc gcaacgggcg ctgacaggcg gcaagaaaat catcaaatga
       61 gagcgtcgaa ggcatcgctt cgcgcatttg tgtcagaaag gcgtccggga aataaacggt
      121 gtgttgggcc acgagcatgt accactggca aaaaaacagg cgcgcagttt agcataaacg
      181 ctccggcgca ggcacgccgg agcagtgggc attatttggg aagcgcagtt ccccattcac
      241 gccactcttt cggttcactt tcctgcaaca ggaagtgttt gccttcctgg gctttcggtg
      301 ccagtggcgt acccggaggc gtggcgaagg cgatgccgcc acggataaac tggttaaagg
      361 tgccggtttt cactacgccg cccgtcagac caaagtccag actgtaaccc gatgccaacc
      421 agaagacgga attgttacgc accaggtgtt gatagcgttt actgatgcgc atcgcaatca
      481 tcacgcgatc tgacaatgtc cccagcgtca ttcctgtaac cgtaccgact tccagaccac
      541 ggaacagcac aggcgtacca atacctaacg aaccggcttc cggcgcttca acaataatgc
      601 ttaagccatc caggtaacgc gaatcagtaa tggtggcctc ttgtaattca aagtcgcggc
      661 gaggattgcc ccggcctggt tcgacgttga tatacggctg gaggatagta tcaagatgct
      721 caacgccagc tgccgaaatt tgcggtgtga ccactgagaa gcgcgtacca ccgcgagcaa
      781 aggtctggac atattccgga tagagcaccg cctttgcctg tacttcattg cgcgcggtaa
      841 tcagatccag cgtctggatt tgcccgatat caataccgag atagcgaatt ggcatcccga
      901 ccgccagttt tccggcatcg aaagcgtgaa gcgtaatctg cccaccaacc gcacgggccg
      961 ctgtttcgga agcatacaga atacgtttgt cgcctttacg ctgactggcg ctggcaccac
     1021 tgaggttatc gaagctaata gcccctttta atgctctgga gagcggggat gcctgtacgg
     1081 tcaggccact accattcagc tgaactttcg ccccgccttc tgcccagaac acgctattgc
     1141 tggtcagaag gttgcgatac tccggcttaa tatgcagatc gatatcaaac gcgttagctc
     1201 gcggacgcac ggtaataact tcaccaactt caaatttacg gtacagcact accgatcctg
     1261 cctgcacatc cggcagcgtc tctgcactca aactcacggt tgtggtgggt aaatcgctaa
     1321 ggctgttctc cagcgctttt tccagattgg catacagtgg atagctggct ttcatctcgc
     1381 ctttatcgcc cggcagaata cgtatcccgc cgttaatcca ttctgaggcg ctggcaccga
     1441 gaaactcaac gccatccagc cccaccttca cgtcgacacg gctgttgacg acaaatttgc
     1501 tatcgccttt taccagttct cgatgctgag gctcgatggc gacggtaaag gtgacgcctt
     1561 tgctggtgag tttacgatcg ataacctggc ctacctgcac gccgtgaaga atgagcggct
     1621 gaccagcatc aataccgtaa ctttccggcg cggtcagggt cagcgtcaga acatcaggtt
     1681 catgcagcag tgctttttcg cctggcacaa caacgaactc tttgcgtggc tcgccatcgc
     1741 cgggtaccaa ctcgaaggtt ttgccagtca gcagggcgct gagattggca tcgctaaggg
     1801 ataatttcgg attacgtaat tcgatgcggg tattttcacg aagcagggta acgacgctgg
     1861 gatcaactgt catttcccca gtgactttac caccaggatt taaatccagt ttagttagct
     1921 gtccgacttc cagtccctga tacattaatg gcgtcgagtc ggcggttaat ccggccccac
     1981 tcggcagttc cagttttatt attacgccac gctggctatg ggcgaggtct tcatacagac
     2041 caaaggtatc ttccgcctcg gcaggtttcg actcttctgg tgaatcgaag gcaatcgcac
     2101 cgttaaccag tgccgccagg ctttccagtt tcaccttcgc gccactgata ctgacattgg
     2161 catcaacgcc ggaaacgttc cagaaacggc tacctttttt caccagatcg gtaaaacgcc
     2221 gctcgatcag gacatcaatc accacgcctt gtttgttggg attgatggca tagtcgtaga
     2281 cttttcccac cgggatcttg cggaaataga ccaatgaacc gctgttcagc gaaccgagat
     2341 cgggggcttg caggtggatc atcagatcgc cattgtccag ccgatatttc ggttgggtat
     2401 cgagtgcgac aaagtgatcc tgctctttac ctttacccgg catcatgccg atatagttcc
     2461 caccgacgag ggcgtccagc ccagagacac ctgccaacga tgcttttggc gtcaccagcc
     2521 agaattgagt ctcttcgcgc agcgcatctt tcatatcgga cttgatgctg accttgactt
     2581 caatcttacg aagatcgtcg ctgaggctga tatcctgcac tgttccgact tcaacgccct
     2641 gataacgaac aggcgtacgg cccgggacaa taccatccgc cgacataaag tcgatggtga
     2701 cggtattacc ccggtcctga taactgtccc aaatcagcca accggcaatc attagcgcga
     2761 tgaaaggcag cagccagaaa ggtgagatac ggcgtttatt tttaatctgc gcttcagtcg
     2821 tcgaagcggg cgtttcctga ctcatgtgca tcccaaagta agcggctgtc cagccattcc
     2881 acagcaagaa tagtcaaaat tacggctgcg ccaaaataaa acgcagccgg tcccatagta
     2941 aaagcgagga tctgatcgcg attaatcagc gacatggtta aagatatgac aaacaggtcg
     3001 agcatcgacc agcgaccaat ccaggtcacc atccgcagta acagaatgcg tgtgcgtaaa
     3061 ccttgctggc atttaaaatg aatgctcaac agtaaagtga acatgacgat cactttagta
     3121 aacggtacca gaatactggc gataaacacg attcccgcaa ccgcaatgtt gctacttgcc
     3181 agcgacataa ttccggaaag aattgtatct tcctgccgtc ctccgttcag ataaatgata
     3241 gaaataggca acaggttggc aggtaacaac aaaacgattg acgctaacag cgccgcccag
     3301 catttttgca aactatgacg gcgacgcagg cgtagcggga tatggcaacg cgggcagcga
     3361 ccacgctgat caggatagcc ggtaaaatgg cacccaagac agacacgaag tttctcgtcc
     3421 ctacgcgtag cggggcgctg cggataaaat cgctcccaca gctcctcgac attaagatgt
     3481 gacaacgtca ccgtcgttaa aatcaccaac gccacaaaag agaacaagcc gacacccgcc
     3541 tggatatggg cataatcctg tacctttata gaagcaacgc caatgccgac caggtagata
     3601 tccagcatta cccactcttt aagtcgctca agcatcagca gtaccggacg taaattcatt
     3661 cccagtcggt taccaaacca taaatatgct atggaggaca ccagaatgag gggggcacca
     3721 ataacgcaga aaaagaccat cgcccccgtt atcgtatcgc cctgtttggt catttgccag
     3781 atgccttgca tgacgttggc gtcgatacga atacctaaca gccagatatg caacagcggt
     3841 tcgccccagg caaacggcat cagcaacagc atggtgaaag ccattgccgc caggcgcgtt
     3901 agcgaccagt cgcgcccgtc gcgaattttt gcctgacagc gcggacaata ggcactctga
     3961 tgagaattta tctcgggcag gctaaacagc atgtcacatt gcgggcaacg ttggtaatca
     4021 ccacgcggca gttcctcgcc gattg
//
LOCUS       DAFATA010000202         3949 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFATA010000202 DAFATA010000000
VERSION     DAFATA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.204"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..301)
                     /locus_tag="LGJ20_004702"
     CDS             complement(<1..301)
                     /locus_tag="LGJ20_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2388685.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGK"
     gene            456..1292
                     /locus_tag="LGJ20_004703"
     CDS             456..1292
                     /locus_tag="LGJ20_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBK2388686.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSAQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNALETRHQQDSQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            1316..2368
                     /locus_tag="LGJ20_004704"
     CDS             1316..2368
                     /locus_tag="LGJ20_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBK2388687.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDSTMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            2414..3214
                     /locus_tag="LGJ20_004705"
     CDS             2414..3214
                     /locus_tag="LGJ20_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK2388688.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            3316..3810
                     /locus_tag="LGJ20_004706"
     CDS             3316..3810
                     /locus_tag="LGJ20_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBK2388689.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            3810..>3949
                     /locus_tag="LGJ20_004707"
     CDS             3810..>3949
                     /locus_tag="LGJ20_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HBK2388690.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQ"
BASE COUNT          936 a         1121 c         1110 g          782 t
ORIGIN      
        1 tctttcctgt ggccgtcgca tccggctggc catcatgcag ttttttaagc tgccgggtca
       61 gcaggtctat ttccttaaag tcaccacctg ttttattctg tttttcagta agctggatga
      121 ggcgcgcatc gatggactgc gtgacacgct gcacgggtgg cgtttcatcc cactggtcgc
      181 gttttttcca cgcataaatc gtgttcgggt ttattcccat cagacgtgat atttctgcgg
      241 gcggataacc ctgccagtaa agttgccgcg cacgctggcg cacaaaagcg tcctgaatca
      301 ttgctccccc tgagtaatta caggaagatt acccgcgcgc gaaaccgttc tccttaaccc
      361 cctgttctgg ccgttttctt acaacaaaag ccctttgtat cagcctgtta cgctttgcca
      421 tcatgactga agaaccagtc agaggggcaa aaactatggc taatgaaaaa aagacatccc
      481 gcaaaaagtt tcgcgtggct gtctccggat caactgttga tggccgtgaa atcagtccgg
      541 tgcatctgcg tgaagccgcc gagaacttca acccggatgt ttacgctgcc cgcgtgaacg
      601 ttgagcacta tctctcgcca tgcccgtcaa gcgaattttc cgcaatgggc gatgtcaccg
      661 cactgagtac ggaagacatt acggaaggtc cgctggccgg acgtactgcg ctgtatgcag
      721 aaatcgaacc gaccgagcgc atgaagcagc ttgtcgcgga cggcaagaaa atctattcca
      781 gtatcgaact gcacccgcag ttctccgtta acgggcgcgc ctatctggtc gggctggcga
      841 tgaccgacac cccggcaagc ctgggcactg agcgcctgaa attcacggca cagcagcgtc
      901 aggcggtgat gacgttcaac agtgcccagg gtgaagcacc gctcatttcc gaagccatcg
      961 agtctgaaat catcgaaatg gcagaacaac gccaggaaga aggcacccag tggtttaacc
     1021 gcgtaatggg gattattggc cgtggccgca aagcggatga cgccagtttc tcccgtattc
     1081 aggaagcggt ggaaggtgtc gcaacgtcac aggccgatat tatcgaccgt tttaatgcac
     1141 tggaaacccg ccatcagcag gacagccaga aaatcacttc actgaccaca gagctggcag
     1201 cactgaagga aaaactgcgc acgcaggacg gcgatccgca gaaccggttc accgcaacgg
     1261 gcgcagcttc cgaccagctg gctgacttct gataagacaa aggagcaaat tttttatgaa
     1321 tctggtgatg tcagatatta cccgcaacaa gctgggttgc tatatggcgc agcaggcgtc
     1381 gcttaacaat atcccggttt ctgcactggt atcgcgattt accgtggaac cctcggtaca
     1441 gcagcgtttt gaaaacgcct caaaggaaag caccgaattt acaaaaagaa ttaacgtgat
     1501 cggcgtgacc gaccagaaag gcgaaaaaat cctcctggat accaccgggc cgattgcgcg
     1561 cacgaatacc agttatgacg gcacaaaacg ccgtaacccg aataacgtgg ttgatctgaa
     1621 aaaccgcaaa taccagtgcg aacaggtgaa ctacgacacg tttatttcat atccgcagct
     1681 tgatgcctgg gcggcacacc ctgattttca gtcccgcatc agcgcacaga ttgcccgaca
     1741 ggtggcactt gaccgcatca tgatcggttt caacggcacg tctcacgcgg atgagtccaa
     1801 cttcagcacc aacaagctgc ttcaggacgt taacgtgggg tggctggagc acatcagaac
     1861 cgacgccagc gaacgcgtta tgaatgacgt gacgctgacc tcccgcaaca tggacaacac
     1921 cgtggcgcac gcgggtaagt atgcgaacgc tgatgcactg gtacaggacg cgcgctcatc
     1981 cctgctggat gaatggcaca aggaagctga cgacctcgtg gtgattatgg ggcgcaacct
     2041 gtttaactcg ctgcgtctgc ccgtgctgaa cagcatcagc ggccagaatc ccaatgcgga
     2101 attacttgcc gggcagctca ttctgtcatc gcgcaccatt ggcgggctgg gcgtgttcct
     2161 tgcgccgttc ttcccggatt caacgatgct gatcacctcg ttcaacaacc tgtcgattta
     2221 ctggcagaaa ggttcaatgc gtcgtctgat gaaagacgaa ccggaataca accgaatcgc
     2281 cacctaccag tccatcaatg acgcttatgt cgttgaagac tatggcaagt gcgcgatggt
     2341 cactggcctg aagttcgccg acagctaatc aactcacggc gggcatcatg cccgcctgta
     2401 acggagagaa caaatgatta ctcctgcaca gcaacactgg cagaacgtga tggcgcagcg
     2461 cgcaggccgg gcgaatgaag gcgtggacca cgccgcgcgt accgcgcatg aagaggtgct
     2521 gtatcgtctg cgtctggcac aggcccggct taagggcgta caggccagaa gcgcgaaagc
     2581 cgccatcaaa aaagagttgt tgccggattt ttccggctgg attgagggaa cgctggaggc
     2641 tgacggcggg cagcaggatg aagtgattgc cacgctgatg gtgtgggcga ttgactgtgg
     2701 cgatcttccg cttgcgctgc gtatcggcgc gtatgtggtc cgtcacaacc tcatcatgcc
     2761 ggataacttt ggccgtactg ctgccacggt actgaccgaa gaaatctgca acccggtact
     2821 gacgcaggcc gggacggatg ccgacgcgga tttatccgcc tttatcgaac cactggacac
     2881 cctccgggag attgtcaccg accaggacat gccggacgaa gtgcgcgcca aattatgcaa
     2941 agcgtgcgcc tttgcccgcc gtggcctgag tgatgcggac agcatggccc tgtcactgaa
     3001 gctgctgcgc gaagcaatgc acctgaaccc gaacgcaggt gtgaaacgcg agattgcaac
     3061 cctttcccgc gccctgaaaa aagccgattc cgcagccgca ccagaagacg ccagcgcaca
     3121 gcaggcgcag gacgaaagca gcaaaagtaa aaagacaacg cggaagcctg caacacgaaa
     3181 aaccaccgcg acgcagaagg cgaagcgcgg ttaacgactg accccgtcag cgggcggcgt
     3241 gcgcggtgtt ccggtttgac tccgtgaccg tttacaccgc gcacccaccg cccgattttt
     3301 tcaggagtga accccatgag tatggttgcc agaaccaacc ccggacccgc agaggacgac
     3361 atcaccgata ccgatgatgg tgatacccgt atttcagcgg gtgcattctg gccggatatt
     3421 gtgctgcgtg aactgcgtct ggcggtacga ctgccgggcc gtgtgaccac ctcccgcctg
     3481 ctgcataccg ccaccggggc cgtggcacac gttacccgcg agctggaagc gtggcagcag
     3541 gaacagcagg cggctggcca tcagacgctg gccgatgttc cggcacccgt aattaacgga
     3601 gaaagcgtca atctctggca ctggcgcaat gctgtttata ccgccacacg cgccctgatt
     3661 ctggagcgtt accgcgatgc ggacacaacg gacaagggcg accgccgggc ggacgctctg
     3721 gatatacaga catcggattt gtggcgtgat gtgagctggg ccatctctga cattctgtgc
     3781 cgcccgcgaa tctttgcgga gttgtgctga tgaaagtgaa ggcactggaa ggcgacaccg
     3841 tggattcgct ctgtttccgg tactacggca cgacgcaggg cgtcaccgaa aaggtgctgg
     3901 atgccaaccc cggactctgt cagcaggtat ttctggacgc cgggcagga
//
LOCUS       DAFATA010000203         3942 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFATA010000203 DAFATA010000000
VERSION     DAFATA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.205"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..145)
                     /locus_tag="LGJ20_004708"
     CDS             complement(<1..145)
                     /locus_tag="LGJ20_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK2388691.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDL"
     gene            complement(169..864)
                     /gene="minC"
                     /locus_tag="LGJ20_004709"
     CDS             complement(169..864)
                     /gene="minC"
                     /locus_tag="LGJ20_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBK2388692.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1384..1752
                     /locus_tag="LGJ20_004710"
     CDS             1384..1752
                     /locus_tag="LGJ20_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388693.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            complement(1872..2273)
                     /locus_tag="LGJ20_004711"
     CDS             complement(1872..2273)
                     /locus_tag="LGJ20_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBK2388694.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            2515..2808
                     /locus_tag="LGJ20_004712"
     CDS             2515..2808
                     /locus_tag="LGJ20_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBK2388695.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2880..3539
                     /locus_tag="LGJ20_004713"
     CDS             2880..3539
                     /locus_tag="LGJ20_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBK2388696.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            3631..>3942
                     /locus_tag="LGJ20_004714"
     CDS             3631..>3942
                     /locus_tag="LGJ20_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBK2388697.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PA"
BASE COUNT          992 a          881 c          964 g         1105 t
ORIGIN      
        1 tcaggtcgag attacgcagg ccgatatcaa aatctatcac gacagttttc tttcccttct
       61 gggccaaacc agtggcgatg gccgcgctgg aggttgtctt accaacaccc cctttgcccg
      121 aagtaacaac aataatgcgt gccatagaaa ttccttgtta aaaagggatc aatttaacgg
      181 ttgaacggtc aaagcgtttt cgactaactg cagtcgcgcc gctttgccat aaaattctgc
      241 tgggatttga tcactcagcc agtattcacc tgcgatggac accagttccg ccatcaggtt
      301 cgtacaaaat atttgcgttt cccggtcacc acttgcccct gcaagcgcac gaccgcgcat
      361 catgccatag acatgaatgt tcccatcggc aatcaattcg gccccagcgc taacgtggct
      421 tgtaacaatc agatcacatt gtggagcata aatacgctga ccggaacgca ccggggtatc
      481 tattaaacgc gtttttgtga ccggcgttgt attttgcgct ggagcctgtg gtgcgggagc
      541 tggacgcggc gctttttcct ttccttccgt caggataggc agccccatct tttcaatttc
      601 ggctttaagt tgcgcatctt tgcagccact tacgccaata acccgcaaac cggttgccga
      661 aaccgcctta tgcatcgctg accagtttac cgggtcttcc agtgcactga cgttgagtac
      721 aacgggggca tgttttaaaa atgcgggggc ctgagcgatt ttgtcttcca gcgcctgatg
      781 gataacctta ggttctgcct catgcagatg aaccacagat aaagtgaagc tactgccttt
      841 aagctcgatt ggcgtgtttg acatcctggc cttactcaat tagctattaa tcatcgccag
      901 cgcgcgatga tgttccgaag actataaggc atgttatagt ctggattata ttgaggcaag
      961 tcaccctccc atttattcag agtaaaagtc tattctgtga taaatggcgc tgattcatag
     1021 cttaaaaaat acccttgtca atcaacccat tgccgtcgta cttttgattg ttcttattta
     1081 cgcttccttt ttccgcaccc taatcgtgac agcatctcca gacctgttta aatatccagt
     1141 attgaggcaa gtgtattttt tcaccacgcc aggctttctt aactttccat tttttgcggc
     1201 atcattaaag aacagatacc cgttatctta atatccgcca cagaattgct ctatcataga
     1261 aaccgcagaa gacgtctgat acttctgtca ggtcatcgta actcgtccag gtcagcggtt
     1321 ttgattacgg attgatgatg aaatgagcat aagtgttgta cggttggatt atcaggatgt
     1381 gctatgaata tgatgcgtat tttttatatc ggattgtcag gtgtggggat gatgttctca
     1441 tcaatggcgt ctggacatga cgctggcgga cttcaatctc cggcgtgcgg cgttgtttgc
     1501 gacccgtata tttgtgtgaa ctcagatggt atttccccag agttaacaag gaaatatctc
     1561 ggcgaaaaag ccgctgaaaa cttacaatca ttacaaggct acgatcccag cgaatttaca
     1621 ttcgctaacg gtgtattttg cgatgttaaa gaaaaattat gtcgtgatga tcgttatttt
     1681 ggtgtggatg gtaagcggag tggaaaaatc aatcaaatca ccacaaagat gttatttatg
     1741 tgtcgtgagt gatgccttgt ttacattatg ttctattcgg cattgtctgc tattcgtcat
     1801 taacctcttg agcgacagag aggagtgggg cgttaacgtt tatgacgggg agagtccccg
     1861 tcggttgaca tttacttaat ttgaatatcg acgttatatt ttttagtttt gtttttacgg
     1921 gcgtcgtttc gggtttgcag cactcgcagc tcatatttgc cagaagcagg gagtgagtat
     1981 tgtccgtgac tatcgagttc tggtgaatat ctggataaat caactgaatc atcgattcct
     2041 ggcccgaaga ggtaagtatc agcgccttca ttagagatac tcacatgcac tttctgacct
     2101 tttttggcat agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc ggagtactga
     2161 gcgctgctat ggcctttgct gaactcaaca ttgacattct tacccgcagc aaagctcgtg
     2221 gaggttagca gtgcaagcag caatacagcc ttactgatgc tcttgatttt cattggtagt
     2281 ctcctgttca gaatgagtac gatgaactgt tataatataa caattcctta cgggagaatt
     2341 ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca ttttttgccg gggggattac
     2401 ttataaaggg gcatggactc aacaaaccgc agaaatctac agctggctgg cttgtggcgc
     2461 gggtttcatt gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt
     2521 tgtgtgattt atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac
     2581 gatttttcgc gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg
     2641 attctgccgc tggatgggcg caagaaactg gtcaatgctg atattgaaaa ggttaaactg
     2701 gcattaaccg agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag
     2761 caacatcttt ccgtcatggg gcagaaaaca gacgacacta acaaataacc gatatccggc
     2821 ggtggcatta tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta
     2881 tgtatcaaca tcataactgg caaggtgcgc tgctggatta tccggtgagt aaagtagtct
     2941 gtgttggcag taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc
     3001 cagtgctgtt tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc
     3061 catccgattt cggttcagtt catcatgaag tcgaactggc ggtgttgatt ggcgcgacgc
     3121 tgcgtcaggc aacggaagag catgtccgca aagccattgc cggttacggc gtggcgcttg
     3181 atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg
     3241 ctaaagcgtt tgataactct tgtccgcttt ccgggtttat ccccgcggcg gaatttaccg
     3301 gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta
     3361 cgactgcgga catgatccat aaaatcgttc cgttgatcgc ttatatgagc aagtttttta
     3421 ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc ccgttgcaaa
     3481 gcggtgatga gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat
     3541 actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag
     3601 gtgcgtttta acgtaatggc ggaacacctg atgagcgatg tacctttctg gcaaagtaaa
     3661 accctggacg aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt
     3721 tgcctgcata aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt
     3781 cgccagctca atattaaaac ttgtcaatgt cggaactacg aacgtcgttt cgagtttgaa
     3841 cccgactgca ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct gccaatgacc
     3901 tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg cg
//
LOCUS       DAFATA010000204         3884 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFATA010000204 DAFATA010000000
VERSION     DAFATA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.206"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(93..863)
                     /locus_tag="LGJ20_004715"
     CDS             complement(93..863)
                     /locus_tag="LGJ20_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK2388698.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(941..1741)
                     /locus_tag="LGJ20_004716"
     CDS             complement(941..1741)
                     /locus_tag="LGJ20_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK2388699.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1982..2896
                     /locus_tag="LGJ20_004717"
     CDS             1982..2896
                     /locus_tag="LGJ20_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2388700.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(2893..3696)
                     /gene="dkgB"
                     /locus_tag="LGJ20_004718"
     CDS             complement(2893..3696)
                     /gene="dkgB"
                     /locus_tag="LGJ20_004718"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBK2388701.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1013 a          937 c          931 g         1003 t
ORIGIN      
        1 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
       61 aaaggcaccg attcccccgg tgcctttttt atttatgctg ctttatgtgc ttctaccatg
      121 atgatatcac tggtgaaaga gccatcattc tgcaaggcaa aatacgttct cacctctgtc
      181 gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc
      241 gcgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
      301 ttggcttcat tgattaacct caaccactca ccgctggcgt agtttcgtac gtgagaggta
      361 tcgcgtaatg cttctaccgt ctgtaaccag atgtcgcgca ctggatgacc cggagacatt
      421 acgtccatca caatcatcct accgccaggt ttcaatatcc ggttcacttc acgcagtgct
      481 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta
      541 tcggcaaatg gcagactttc ggcataaccc tggcgggtgg tgatattttt cagttgccgg
      601 acttcggcag cttgtgccac gacatccagc atgtgggcag ataagtcata cgccaccacc
      661 gtgctcacgt tttgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt
      721 acacttgcac caggataatc agccagacgc accgccaggc gctgtaaatc tcgtccggat
      781 gcatgtacgg tactggtcag atattcgcag gcttgagagc taaactgctt ttctacgtgg
      841 tcatggtggg attgtgttgt cattgcgctg tccttttgtt gtttgagtat aaaaggacag
      901 cgccaaacgt tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg
      961 gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccaat acagaagctt
     1021 cgctgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcggccaaac gcccgacggc
     1081 gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag cgatataacg
     1141 cgttcatcct tctacggctc caggcattga aatctcccgc cataatgacc gggccgctgt
     1201 ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc acgcccagac
     1261 tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aatggataga
     1321 ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc
     1381 aatacactgg atgcgccgcc gaaagggtca ttacaccgga aggatgttgt ggcagcacga
     1441 aagcgggcac ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg
     1501 ttgtctgcgc ttcctgcaat aacaccagat gtgcatcttt gccgtagttc tttaataccg
     1561 acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt
     1621 cggtacttaa cggttctcca ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg
     1681 gtaagatcct ttccgcaggt tgtccagcaa catagcgcat ggcataggtg ttttttcgca
     1741 cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactgtt
     1801 atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct
     1861 tacacaggtc agaattttgc tcattattga gaacattaaa tcggatgaca tatcacgcat
     1921 cctgatggtt taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa
     1981 aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtagaaa gcggcagctt
     2041 tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg cagtgaaaaa
     2101 gctggagatg aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac
     2161 ggaagaaggc cagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga tggcagcggc
     2221 agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttgcgca tcgatgccgc
     2281 aactccagtg gtgctgcact ttctgatgcc gttgattaag cctttccgtg aacgctatcc
     2341 ggaagtcact ttatcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt
     2401 ggatgtcgcg atacgcgctg gtacgttaac ggattccagc ttacgtgcca ggccgttatt
     2461 taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga
     2521 aacgatcgac gatttaaagc aacatatttg cctgggattc actgaacccg cttccctcaa
     2581 tacctggccg atagcccgta gcgatggaca attacatgag gtgaagtacg gtttgtcatc
     2641 caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc
     2701 cgactacatg atcgacaaag aaatcgctcg cggagaattg gtggagttaa tggcagataa
     2761 agtgttgcca gtggaaatgc cattcagtgc cgtctattac agcgaccgtg cggtaagtac
     2821 gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc ccggaggagc
     2881 tgtcagagag gcttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt
     2941 gcaatccagt gcggcgatcg cttttttatc ttcggcatca agctgtaaat tttgtgcctt
     3001 aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt aaccttcccc
     3061 catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc
     3121 aataacctca tctttcaggg ccttaccata cgccagcgtc atataggaag taatatggat
     3181 gccgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat
     3241 ctggttagta gcgatgtttt cagcaccaac agcagcaatc gctttttcca tcaacgggat
     3301 cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc
     3361 ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag
     3421 atcaacataa tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc
     3481 tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc
     3541 actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat
     3601 tgcgcgataa ccaagttcaa gcgccgttat cacagatgaa ataacaacgt cgtctttcag
     3661 acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt
     3721 tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaagagcgt aaaatgcaga
     3781 ggatttttgc gattctggca ataatagata tacagacgaa aaaaaacctc gagcgttaac
     3841 tcgaggcttc ttaataagtg gcggaacgga cgggactcga accc
//
LOCUS       DAFATA010000205         3814 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFATA010000205 DAFATA010000000
VERSION     DAFATA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.207"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..389
                     /locus_tag="LGJ20_004719"
     CDS             <1..389
                     /locus_tag="LGJ20_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBK2388702.1"
                     /translation="EFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLV
                     EAMQPDDCLVVMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATV
                     CEFFRAPPPQNGRSFLSSLRFAGDTL"
     gene            386..1264
                     /locus_tag="LGJ20_004720"
     CDS             386..1264
                     /locus_tag="LGJ20_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBK2388703.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            1275..1628
                     /locus_tag="LGJ20_004721"
     CDS             1275..1628
                     /locus_tag="LGJ20_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBK2388704.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            1640..2944
                     /locus_tag="LGJ20_004722"
     CDS             1640..2944
                     /locus_tag="LGJ20_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBK2388705.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSASVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            2956..>3814
                     /locus_tag="LGJ20_004723"
     CDS             2956..>3814
                     /locus_tag="LGJ20_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK2388706.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFH"
BASE COUNT          807 a          935 c         1113 g          959 t
ORIGIN      
        1 acgaatttaa cacgcatccg acggcgttta tttgcatcaa tatccaggaa accgacctcg
       61 ctggtcatgc agaagacgtc gcacgttatg ccgaacgttt gcaggtcgtt gaccgtaacc
      121 ttgcccggct tgttgaggcg atgcagccag atgattgcct ggtcgtgatg gcggatcacg
      181 gtaacgatcc gaccattggt cacagccacc atacccgtga agtggtgcca gtgctggttt
      241 atcagcaagg gctggtccac acgcaactcg gtgtacgcac cacgctttct gatgtggggg
      301 ctaccgtgtg tgaatttttc cgtgcgccac cgccacaaaa tggtcgctct tttctttcct
      361 ccctccggtt tgcaggagac accctatgag tattgatccg acgggttaca ccctggccca
      421 tgagcatctg catattgatc tctccggctt taaaaacaac gtggactgcc gccttgatca
      481 gtatgcgttc atttgccagg agatgaacga cctgatgacc cggggcgtgc gtaatgtgat
      541 tgagatgacc aaccgttaca tggggcgcaa tgcgcaattt atgctcgatg taatgcgcga
      601 gacggggatc aacgtggtgg cctgcaccgg ttattaccag gacgcttttt tcccggagca
      661 tgtggcgacc cgcagcgtgc aggaactggc gcaggagatg gtcgatgaaa ttgaacaggg
      721 tatcgatggc accgacctga aagccgggat tatcgcggag atcggctcta gcgaagggaa
      781 gattacgccg ctggaagaga aggtgtttat tgccgctgcg ctggcgcata accagaccgg
      841 acgcccgatc tccacgcata cttcgttcag caccatgggg ctggaacaac tggctctgtt
      901 gcaagcccac ggagttgatc tttcgcgcgt caccgttggt cactgcgatc tgaaagacaa
      961 tctcgacaac attttgaaga tgatcgatct cggcgcgtac gtgcagttcg acaccattgg
     1021 taagaacagt tactacccgg acgaaaagcg cattgcgatg ctccacgcgc tacgtgaccg
     1081 tgggttgctg aaccgcgtca tgctgtcgat ggatattacg cgccgctccc atttaaaagc
     1141 caatggtggt tatggctatg actatttatt gaccaccttt attccgcaat tgcgccagtc
     1201 aggattcagt caggccgatg tggatgtgat gttacgtgaa aatccctctc aatttttcca
     1261 ataaggacag actcatgaaa aagattggcg ttgcaggctt acagcgtgag cagattaaaa
     1321 aaaccattga agcgacggct cctggcagtt ttgaagtttt cattcacaac gacatggaag
     1381 cggcaatgaa agtgaaatcc gggcaactgg attactacat cggtgcgtgt aataccggtg
     1441 cgggcgcggc attgtcgatt gccatcgcgg tgattggcta taacaaaagt tgcaccattg
     1501 ccaaaccagg cattaaagcg aaagacgagc atatcgccaa aatgatcgcc gaagggaaag
     1561 tggcgtttgg cctttccgtt gagcacgtcg aacacgcgat tccgatgctg attaaccatc
     1621 tgaaataaaa ggcacgacta tggatctgta tattcagatt atcgtggtgg cgtgcctgac
     1681 gggtatgaca tcgcttctgg cgcatcgctc ggcgtctgtt tttcatgacg gcatccgccc
     1741 gatcctgccg caactgattg aaggctatat gaaccgtcgc gaggcgggga gtatcgcttt
     1801 tggtctgagc attggttttg tggcctcggt ggggatctct tttaccctga aaaccgggct
     1861 gctcaacgca tggttactct ttcttcctac cgatatcctc ggcgtcctgg cgataaacag
     1921 cctgatggcg tttggtcttg gcgctatctg gggcgtgttg atccttactt gcctgttgcc
     1981 agtaaaccag ctgctgaccg cgctgccggt ggatgtatta ggtagcctgg gggaattaag
     2041 ctcgccggtg gtttcagctt ttgcactgtt cccgctggtg gcgattttct accagtttgg
     2101 ctggaagcaa agtctgatcg ccgccgtggt ggtactgatg acccgtgtgg tagtcgtgcg
     2161 ctatttccca catcttaacc ctgaatccat cgaaatcttt attggcatgg tgatgctgct
     2221 ggggatcgcg ataactcacg acctgcgtca tcgtgatgaa aatgacattg atgccagcgg
     2281 gctttcggtg tttgaagaac gcacgtcacg gattatcaaa aacttaccct atatcgccat
     2341 cgtgggagca ttgattgccg ccgttgccag catgaagatt tttgctggca gtgaagtgtc
     2401 gatcttcaca ctggagaaag catattccgc aggcgtaacg ccggaacaat cgcaaacgct
     2461 gattaatcag gcggctctgg cagaatttat gcgcggactg gggtttgtgc cgttgattgc
     2521 caccaccgcg ttagcaacgg gtgtgtatgc agttgcgggc tttacctttg tttatgcggt
     2581 gggctatctc tcgccgaatc cgatggttgc agcggtatta ggtgcagtgg ttatttcggc
     2641 agaagtttta ttgcttcgtt cgatcggcaa atggctgggg cgctatccgt cggtgcgtaa
     2701 tgcgtcggat aacatccgta acgccatgaa tatgctgatg gaagtggcgc tgctggtcgg
     2761 ttcgattttc gcagcaatta agatggcggg ttataccgga ttctctatcg cggttgccat
     2821 ttacttcctc aacgaatccc tgggccgtcc ggtacagaaa atggcggcac cggtcgtggc
     2881 agtaatgatc accggtattc tgctgaatgt tctttactgg cttggcctgt tcgttccggc
     2941 ttaaggaagg cacctatgaa gacgtttcct ctgcaaagcc tgacgattat tgaggcgcag
     3001 caaaagcagt ttgcgctggt ggatagcatt tgtcgccatt tccccggcag cgagtttctt
     3061 actggcggtg atttaggctt aacgccagga ctgaatcaac cgcgcgttac ccagcgtgtg
     3121 gaacaggtgc tggctgatgc atttcacgca caggctgcgg cgctggtgca gggcgcgggg
     3181 actggcgcga ttcgcgccgg gctggcggct ttgctcaaac cggggcagcg tcttctggtg
     3241 catgacgcgc ctgtttaccc gacgacacgg gttattattg agcagatggg gctgacgctt
     3301 attactgttg atttcaatga cctgtcggca ctgaagcagg tcgtcgacga gcaacaaccg
     3361 gatgcggcgc tggtgcagca tacgcgccag cagccgcagg acagctacgt gctggcagat
     3421 gtgctggcaa cgttgcgcgc ggcaggtgtt ccagcgttaa ccgatgacaa ctatgcggtg
     3481 atgaaggtgg cgcgaatcgg ctgtgaatgc ggcgcgaatg tctcgacatt ttcctgcttc
     3541 aagctatttg ggccagaggg tgttggtgca gtggtcggcg atgctgatgt tatcaaccgt
     3601 attcgcgcca cgctttactc cggcggtagc cagatccagg gcgcacaggc gctggaagta
     3661 ttgcgtggtc tggtgtttgc gccagtgatg cacgcggtgc aggcaggggt atctgaacgg
     3721 ttgctggctt tgcttaacgg tggtgcggtg ccggaagtga aaagcgcggt gattgctaat
     3781 gcgcagtcga aggtgttgat tgtcgagttt catc
//
LOCUS       DAFATA010000206         3810 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFATA010000206 DAFATA010000000
VERSION     DAFATA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.208"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="LGJ20_004724"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="LGJ20_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK2388707.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="LGJ20_004725"
     tRNA            complement(324..399)
                     /locus_tag="LGJ20_004725"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="LGJ20_004726"
     tRNA            complement(406..480)
                     /locus_tag="LGJ20_004726"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="LGJ20_004727"
     tRNA            complement(597..681)
                     /locus_tag="LGJ20_004727"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="LGJ20_004728"
     tRNA            complement(690..765)
                     /locus_tag="LGJ20_004728"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="LGJ20_004729"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="LGJ20_004729"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK2388708.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="LGJ20_004730"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="LGJ20_004730"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBK2388709.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..>3810)
                     /gene="murB"
                     /locus_tag="LGJ20_004731"
     CDS             complement(3068..>3810)
                     /gene="murB"
                     /locus_tag="LGJ20_004731"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK2388710.1"
                     /translation="LQEGMPGLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVE
                     LATGKQVRLTAKECRFGYRDSIFKHEYQDRFAILAVGLRLPKEWQPVLTYGDLTRLDP
                     TTVTPQQVFNAVCHMRTTKLPDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQ
                     AGGSVKLAAGWLIDQCQLKGMQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKV
                     GEKFNVWLEPEVRFIGASGEVSAVETIS"
BASE COUNT          991 a          961 c          842 g         1016 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cacctgttta ctcacatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 aaatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctaaaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag
//
LOCUS       DAFATA010000207         3775 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFATA010000207 DAFATA010000000
VERSION     DAFATA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.209"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..2787
                     /gene="clpK"
                     /locus_tag="LGJ20_004732"
     CDS             <1..2787
                     /gene="clpK"
                     /locus_tag="LGJ20_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock survival AAA family ATPase ClpK"
                     /protein_id="HBK2388711.1"
                     /translation="NGRHSTMLLCDDHYRQLVRQQKRTVSPLEALFGSRSGLFEDFLG
                     SDFFRIGDDATPVAADTDDVVDASFGEPAAAGSGVPRRRGSGLASRISEQSEALLQEA
                     AKHAAEFGRSEVDTEHLLLALADSDVVKTILGQFKIKVDDLKRQIESEAKRGDKPFEG
                     EIGVSPRVKDALSRAFVASNELGHSYVGPEHFLIGLAEEGEGLAANLLRRYGLTPQAL
                     RQQVSKVVGKGAEDGRAETPTNTPELDKYSRDLTKMARDGKLDPVIGRAQEIETTIEV
                     LARRKKNNPVLIGEPGVGKTAIVEGLAQRMVAGEVPETLRDKRLVELNINAMVAGAKY
                     RGEFEERVQKVLKEVTEHQGELILFIDEVHTIVGAGQGGGEGGLDVANVFKPMMARGE
                     LNLIGATTLNEYQKYIEKDAALERRFQPVTVPEPTVAQTIMILRGLRDTFEAHHKVSI
                     SEDAIIAAAELSDRYITARFLPDKAIDLLDQAAARVKLSATARPVAVQELESELHQLR
                     REQDYVAARKQYDQAAELGKRIEAKEAELKKLVENWERERASGSAEVKAEHVAQIVSR
                     LTGIPVNELTVEEREKLLHLEQRLHERLVGQDEAVRAVADAVRLSRAGLREGSKPVAT
                     FLFLGPTGVGKTELAKALAESIYGDEHALLRIDMSEYGERHTVARLVGAPPGYVGYDE
                     GGQLTEKVRRKPYSVLLLDEIEKAHPDVYNILLQVFDDGRLTDGKGRVVDFTNTIIIA
                     TSNLGSDIIQRRLKARGAADEEYEKTKAEVMDVLRGHFRPEFLNRIDEIIVFHALGKE
                     EIRHIVGLQLDRVARSAASQGVTLTFDQTLIDHFAEEGYKPEFGARELKRLIRSELET
                     ALAREMLGGGIGKGDHASARWDDKAERVVFERKEPPQTPAEPEQPDAAKATETPHGDA
                     GKGSRKKKSASDAS"
     gene            2787..2978
                     /locus_tag="LGJ20_004733"
     CDS             2787..2978
                     /locus_tag="LGJ20_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388712.1"
                     /translation="MAGEAAVSDPIGLAWAASLASIAVAVVAAGHAVVYKRDPRSATP
                     WGLLISGGTALPLAGHPFA"
     gene            3008..>3775
                     /locus_tag="LGJ20_004734"
     CDS             3008..>3775
                     /locus_tag="LGJ20_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HBK2388713.1"
                     /translation="MATRLSRNKAHGKKNQWNTGYWIVAFLLLLSLQSYWQTAKTVEP
                     VPYSEFEKALAEVRVAEVLVSDRTVTGRLKSPDSRGKTTIVATRVEPDLADRLSKYDV
                     PYARVLESTWLRDVLSWILPAVAFFGVWFFLFRRFAEKQGMGGFLNIGKSRAKVFVEK
                     NTGVTFADVAGGDEAKAELVEIVDFLKNPQDYGRLGARIPKGVLLVGPPGTGKTLLAK
                     AVAGEAVVPFFSISGSEFVEMFVGVGAARVRGIGGHDE"
BASE COUNT          711 a         1160 c         1303 g          601 t
ORIGIN      
        1 aacggtcgtc acagcaccat gctgttgtgc gacgaccatt accggcaatt agtgcgccag
       61 caaaagcgca ccgtttcgcc gctggaagcc ttgttcggtt cgcgcagcgg cctgttcgag
      121 gacttcctcg gcagtgactt cttccgcatc ggcgacgacg cgacgccagt tgccgccgat
      181 accgatgacg tggtcgatgc ctcgtttggc gagcccgccg ccgcaggttc gggtgtgccg
      241 cgccgtcgcg gcagtgggct ggccagccgc atcagcgaac agtcggaagc cttattgcag
      301 gaggccgcca aacacgctgc cgaatttggc cgctccgagg tggataccga acatctgctg
      361 ctggcgctcg ccgacagcga cgtggtcaag accatcctgg gtcagttcaa gatcaaggtc
      421 gatgacctca agcggcagat cgagtctgag gccaagcgcg gggacaagcc cttcgagggc
      481 gagatcggcg tgtcgccgcg cgtgaaggat gcgctcagcc gcgccttcgt agcctccaat
      541 gaactcggcc attcttatgt cggtccagag catttcctga tcggtctggc cgaggaaggc
      601 gaagggctgg ccgccaacct gctgcgccgc tacggcctga cgccgcaggc gctgcgccaa
      661 caggtcagca aggtggtcgg caagggcgcc gaggatggcc gcgctgagac gccgaccaac
      721 acgccagaac tcgacaagta ctcgcgcgac ctgaccaaga tggcgcgcga cggcaagctc
      781 gacccggtga tcggccgcgc gcaggagatc gaaaccacca tcgaggtgct ggcccggcgc
      841 aagaagaaca acccggtgct gatcggcgag ccgggtgtgg gcaagaccgc catcgtcgaa
      901 gggctggcgc agcgcatggt ggcgggtgaa gtgcccgaga ccttgcgcga caagcgcctg
      961 gtggaactca acatcaacgc catggtggcc ggcgccaaat atcgcggcga gttcgaggag
     1021 cgcgtgcaga aggtgctgaa ggaggtgacc gagcaccagg gcgagctgat tttgttcatc
     1081 gacgaggtgc acaccatcgt cggtgccggc cagggcggtg gcgaaggcgg gctggacgtg
     1141 gccaacgtgt tcaaaccgat gatggcgcgc ggtgaactca acctgatcgg cgccacgacg
     1201 ctgaacgagt accagaaata catcgagaag gatgccgcac tggagcggcg cttccagccg
     1261 gtgacggtgc ccgagccgac ggtggcccag accatcatga ttctgcgcgg cctgcgcgac
     1321 accttcgagg cgcaccacaa ggtcagcatc tccgaggacg cgatcatcgc ggcggccgag
     1381 ttgtccgacc gctacatcac ggcgcgcttc ctgccggaca aggcgatcga cctgctcgac
     1441 caggcggccg cgcgcgtgaa gctgtcggcc acggcccggc cggtggccgt gcaggagctg
     1501 gagtccgaac tgcaccagct gcggcgtgaa caggattacg tggccgcgcg caagcagtac
     1561 gaccaggccg ccgagctcgg caagcgcatc gaagccaagg aggccgagct caagaagctc
     1621 gtcgagaact gggagcggga gcgggcctcc ggcagtgcag aggtcaaggc agaacacgtg
     1681 gcgcagatcg tctcgcgcct gaccggcatc ccggtcaacg agttgacggt ggaagagcgc
     1741 gaaaagctgc tgcacttgga acaacggctg cacgagcgcc tggtgggaca agacgaggcg
     1801 gtccgtgccg tggccgatgc cgtgcggctg tcccgcgccg gcctgcgcga aggcagcaaa
     1861 ccggtggcta ccttcctgtt tctgggcccg accggggtgg gcaagaccga gctcgccaag
     1921 gcattggccg aatcgatcta cggcgatgag cacgccctgt tgcgcatcga catgtcggaa
     1981 tatggcgaac gccataccgt ggcgcggctg gtgggcgcgc ctccgggcta tgtcggttac
     2041 gacgaaggcg gtcagctcac cgagaaggtg cggcgcaagc cctacagcgt gctgctgctc
     2101 gacgagatcg agaaggcaca ccctgacgtc tacaacatcc tgctgcaagt gttcgacgac
     2161 ggtcgcctca ccgacggcaa gggccgggtg gtggatttca ccaacaccat catcatcgcc
     2221 acgtccaacc tgggttcgga catcatccag cgacggctga aggcgcgtgg ggcggccgac
     2281 gaggagtacg aaaagaccaa ggccgaggtc atggacgtgc tgcgcggcca cttccggccc
     2341 gagttcctca accgcatcga cgagatcatc gtcttccatg cgctgggcaa ggaggagatc
     2401 cgccacatcg tcggcctgca gctcgatcgc gtggcgcgca gcgccgccag ccagggcgtg
     2461 acgctcactt tcgatcagac gctgatcgac catttcgcgg aagaaggcta caaacccgag
     2521 ttcggtgcgc gtgaactcaa gcgcctgatc cgcagcgagc tggaaactgc gctggcgcgc
     2581 gagatgctcg gtggcggcat cggcaagggc gatcacgcca gcgcacgctg ggacgacaag
     2641 gccgaacgcg tggtgttcga acgcaaagag ccaccgcaga ccccggccga gccggagcag
     2701 ccggatgccg cgaaggcgac cgagacgccg cacggcgacg ctggcaaagg ctcgcgcaag
     2761 aagaagtcgg cgagcgacgc atcttgatgg cgggcgaggc tgccgtgtcc gaccccatcg
     2821 gcctggcatg ggcagcctcc ctggcctcga tcgcggtggc ggtcgtggcc gcgggtcacg
     2881 cggtcgtcta caagcgtgat ccgcggtcgg ccacgccgtg ggggctgctc atcagcggcg
     2941 gcactgctct cccgctcgct ggccatccgt ttgcgtgacg gtgcggcccg tctgttcacc
     3001 tgatcctctg gcaactcgac tttcgaggaa caaggcccat ggaaaaaaga atcaatggaa
     3061 taccggctac tggatcgttg ccttcctgct gttgctgagt ttgcagagct actggcagac
     3121 ggcgaaaacc gtcgagccgg tgccctacag cgagttcgag aaggcgctgg ccgaggtgcg
     3181 cgtggccgaa gtgctggtgt cggatcgtac ggtgaccggg cgcctgaagt cgccggacag
     3241 ccggggcaag acgacgatcg tggcaacgag ggtcgaaccc gatcttgccg atcgcctgtc
     3301 gaagtacgac gtgccctatg cccgcgtgtt ggagagcacc tggctgcgcg atgtgctgtc
     3361 gtggatcctg ccagcggtag ccttctttgg cgtctggttc ttcctgttcc gccgcttcgc
     3421 cgagaaacag ggcatgggcg ggtttctcaa catcggtaag agccgcgcca aggtgttcgt
     3481 ggaaaagaac accggtgtga cgttcgccga tgtggcgggg ggggatgagg ccaaggcgga
     3541 gctggtcgag atcgtcgatt ttctgaagaa tccgcaggac tatggccgtc tcggggcgcg
     3601 cattcccaag ggcgtgttgc tggtcggccc accgggcacc ggcaagacat tgctggccaa
     3661 ggctgttgcc ggcgaggcgg tggtgccctt cttctccatc tccggatcag agtttgtcga
     3721 gatgttcgtc ggcgtgggtg cggcccgtgt gcgcggcatc ggcggccacg acgag
//
LOCUS       DAFATA010000208         3702 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFATA010000208 DAFATA010000000
VERSION     DAFATA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.210"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(17..214)
                     /gene="yacG"
                     /locus_tag="LGJ20_004735"
     CDS             complement(17..214)
                     /gene="yacG"
                     /locus_tag="LGJ20_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBK2388714.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(224..967)
                     /gene="zapD"
                     /locus_tag="LGJ20_004736"
     CDS             complement(224..967)
                     /gene="zapD"
                     /locus_tag="LGJ20_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBK2388715.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            complement(967..1587)
                     /gene="coaE"
                     /locus_tag="LGJ20_004737"
     CDS             complement(967..1587)
                     /gene="coaE"
                     /locus_tag="LGJ20_004737"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK2388716.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            1612..1656
                     /locus_tag="LGJ20_004738"
     CDS             1612..1656
                     /locus_tag="LGJ20_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388717.1"
                     /translation="MIIKTLKMSARKRN"
     gene            1812..2855
                     /gene="guaC"
                     /locus_tag="LGJ20_004739"
     CDS             1812..2855
                     /gene="guaC"
                     /locus_tag="LGJ20_004739"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK2388718.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(2890..>3702)
                     /gene="hofC"
                     /locus_tag="LGJ20_004740"
     CDS             complement(2890..>3702)
                     /gene="hofC"
                     /locus_tag="LGJ20_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBK2388719.1"
                     /translation="TGELTGKLDECCFELARQQKAQRQLTDKVKSALRYPIIILAMAI
                     MVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSWLLVLFGFLLAIANK
                     LLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGITFLQGVESVRET
                     MRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTGEASGSLDLMLDNLA
                     HHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHLGDAMSGMG"
BASE COUNT          898 a          953 c          939 g          912 t
ORIGIN      
        1 cggcatcaga caaatgtcac tgctttggct cttcgctcca gtcatcgctt tcggaaagat
       61 cgccactgct ggggattcgt ttttcttcag cagcccattc tccgaggtcg atcagctgac
      121 aacgtttgga gcaaaatggc cgaaacgggc tgatttcacc ccacaccacc gttttcccgc
      181 aggttgggca attcaccgta atagtttctg acattttcac tccttagcaa caggccagtt
      241 cgaaatccag acgttccggt acctgtccgt tttcagtgtc cagcggcata aaacgaatgg
      301 caaaacggct cttatgtccg gaaatttgcg gataaagctg tgaatcgagc gacagattca
      361 ggcgcagcaa gtcggcatcg ccaccgttat cctgataaaa accattcagg ctggtttgtt
      421 tacggaaggg agccgactgg cgaattaaat ccagcaccat ggtaagtgcc tgggtgagcg
      481 ggttcaggct ggcaatccag gtttctacct ggctgtcgcg ctgcgcctgg ggtagatgca
      541 gccaaatgtg caatgtaggt aaatcaaagc tgcaacagcc gcctgggatg ctcagtcgct
      601 gacgcaccag agcaatcaaa cgatcttcac gcagaaattg cccgatacgc ggcgcggaaa
      661 ttaatacgct ccccgccgct tttaactgct gaattaatgc ttcaatacgg ctctggtcca
      721 cgccaggcac gccaatccag gtctggagtt tacgttgctg ccggtcaagt tctttcaaca
      781 gctcagtgcg gacttcgccg cgctcgaaaa catccagtaa ttcactgaca ttacggaaga
      841 aatgcagcgc gccagcgtgg tcaacgatgg gtaaattaac ggtgagttgc tgaatcaaaa
      901 actcaatgcg cagccatgta cgcatttttt catttagtgg atgttcaaaa aggacctggg
      961 tctgcattac ggtttttcct gtgagacaaa ctgcgacgca agctgcaaat agtgtgcgtg
     1021 caggcgggca acatccgatg cgatagcatc cggtgcgccg ttattatcaa tgacgtcatc
     1081 tgccacggca aggcgggctt cgcgcgttgc ctgagcagca aggatttgtt cgacatgctc
     1141 gcgagttaca tcatcgcgct gcatggtgcg cttaagttgc gtttctgggc tgacatccac
     1201 caccagcact cgattcgctt ttttatacag tgagttttct accagcaatg gcacaaccca
     1261 cagtacatag ggggaagtag cttgctggat ctggtgttgc gtctcttgct gaatcagcgg
     1321 atgcagcagg gcgttaagcc agtttttctc ttccgggttg gcgaagatcc gctcgcgcaa
     1381 ggcccggcgc tgcaatgttc catcagcagc aatcatgtta gcgccaaagt gatcagcaat
     1441 ggcatgtagc gcaggtgcac ctggttcaac cacctgacgc gcaataatat cggcatcaat
     1501 gacgttaatt ccgagatcag caaacgcatt ggcaacggta ctcttgccac tgccaatgcc
     1561 tcccgttaag gcaactatat acctcataat ttatattccc gggaattcat catgattatc
     1621 aaaacgttaa aaatgagtgc acgaaagcga aattgatgaa acgttcgctc actatttacc
     1681 aggtaaattt atgggattgt agcgtaaaaa aagacaattt cgcagtcttg cgccgcattg
     1741 attagtgcgt atgatagcgt cactggagtt gcgctcttac ccttatagcc attaacccca
     1801 ggaatccgca catgcgtatt gaagaagatc tgaagttagg ttttaaagac gttctcatcc
     1861 gccctaaacg ctccactctt aaaagccgtt ccgatgttga actggaacgt caattcacct
     1921 tcaaacattc aggtcagagc tggtccggcg tgccaattat cgccgcaaat atggacaccg
     1981 taggtacatt ttctatggcc tctgcgctgg cttcttttga tattttgact gctgtgcata
     2041 aacactattc tgtcgaagag tggcaagcgt ttatcaacaa ttcttccgct gatgtgctga
     2101 aacatgtgat ggtttctacc ggtacgtctg atgcggattt cgaaaaaact aaacagattc
     2161 tcgacctgaa cccggcatta aacttcgttt gtattgatgt ggcgaatggt tattccgaac
     2221 acttcgtgca gttcgttgcg aaagcgcgtg aagcgtggcc gaccaaaacc atttgtgctg
     2281 gcaacgtagt gactggtgaa atgtgtgagg agcttatcct ctccggtgct gatatcgtta
     2341 aagttggcat tggcccaggt tctgtttgca caactcgcgt caaaacaggc gtcggttatc
     2401 cgcagctttc tgcggtaatc gaatgtgccg atgcggcgca cggtctgggc ggaatgatcg
     2461 tcagcgacgg tggctgcacc acgccgggcg atgtcgcaaa agcctttggc ggcggtgccg
     2521 atttcgtcat gcttggcggc atgctggcgg gccacgaaga gagcggcggt cgcatcgttg
     2581 aggagaacgg cgagaaattt atgctgttct acggcatgag ctccgagtct gcgatgaaac
     2641 gtcacgttgg cggcgttgcg gaatatcgcg cagcagaagg taaaaccgtt aagctgccgc
     2701 tgcgaggccc ggttgaaaat accgcgcgtg atattttggg cggcctgcgt tcagcttgta
     2761 catacgttgg ggcttcacgc ctgaaagaac tgaccaagcg caccacattt atccgtgtgc
     2821 aggaacaaga aaaccgcatc ttcaacaacc tgtaatcttc caacgctggt gtggagcaac
     2881 acgccagcgt tatcccatcc cactcatcgc atcgcctaaa tggaaaattg gcagatacat
     2941 cgccaccacc agcgtaccga taattcctcc cgttatgatc agcaacgccg gttccagtaa
     3001 ggctgcgagg ttatccgcca gcgccattgt gttatcccga tgatgatggg cgaggttgtc
     3061 taacatgagg tccagcgagc cggatgcctc tcctgttctc actaattgca aacagagcgg
     3121 gctaaactcc ccggtatttt ttagcgccag ccagatggga tgaccgttac tgatatcgtg
     3181 ctggatttgt gtcagaagtt gcacccagta cgggcagcgc attgtttctc tgacgctctc
     3241 tacgccctgc aaaaaagtaa tgcctgcact ttgtgtcagc gccagaatcg taaagatctg
     3301 cgtgagtttt tgtccccgca tcagtgaacc cataatcggg attcgtaaca gcaatttctg
     3361 ccgcactata agccaggtcg gtcggcgcat cagcaactta ttggctatcg ccagcagaaa
     3421 gccgaacaac accagcagcc agctccattc gccactaaag tctgccagcg tcatgatccc
     3481 ctgcgttagt gccggtagtg gggtgttgaa ggtcttatag atagcggcaa actccggcag
     3541 aacaaaatgc agcattgcca caaccaccat gattgccatc gctaaaatga tgatgggata
     3601 acgtaacgct gatttcactt tgtcggtcaa ctgacgctgg gctttttgct gacgcgccag
     3661 ttcaaagcag cattcatcca gcttaccggt cagttcaccc gt
//
LOCUS       DAFATA010000209         3631 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFATA010000209 DAFATA010000000
VERSION     DAFATA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.211"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..262
                     /gene="yneK"
                     /locus_tag="LGJ20_004741"
     CDS             <1..262
                     /gene="yneK"
                     /locus_tag="LGJ20_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBK2388720.1"
                     /translation="FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLK
                     LFVKNETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            412..1602
                     /gene="ydeA"
                     /locus_tag="LGJ20_004742"
     CDS             412..1602
                     /gene="ydeA"
                     /locus_tag="LGJ20_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBK2388721.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIAALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(1627..2292)
                     /gene="marC"
                     /locus_tag="LGJ20_004743"
     CDS             complement(1627..2292)
                     /gene="marC"
                     /locus_tag="LGJ20_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBK2388722.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2504..2938
                     /gene="marR"
                     /locus_tag="LGJ20_004744"
     CDS             2504..2938
                     /gene="marR"
                     /locus_tag="LGJ20_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBK2388723.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2958..3341
                     /gene="marA"
                     /locus_tag="LGJ20_004745"
     CDS             2958..3341
                     /gene="marA"
                     /locus_tag="LGJ20_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBK2388724.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3373..3591
                     /gene="marB"
                     /locus_tag="LGJ20_004746"
     CDS             3373..3591
                     /gene="marB"
                     /locus_tag="LGJ20_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBK2388725.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
BASE COUNT          932 a          895 c          859 g          945 t
ORIGIN      
        1 atttttcgcc aatatgttat tgcactatca ccaatttatg aatcccaatg aaagtgattt
       61 gaatgacgtg ttgatgccag catcattaag tgatgataaa attatcaaac atatggcgcg
      121 caggaccctc aaactgtttg taaaaaatga aacaccgcca aaagttactc acgaagacct
      181 ggtgaaaaac aggcctcgct cccctgtcag accacctata cccgcaaccg ccaaaacgcc
      241 agacctccct gaacgtcatt aaaccgtgat gttaccgact ctctgacgcg tgaaagaatc
      301 agcgtcagag aaacggaaaa cgcgatccag atcacaaatg cattgtattc acatcattaa
      361 ccgttttaag atcatttcat cactttttcg caactcaccc gataatctgt tatgacaaca
      421 aacactgttt cccgcaaagt ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc
      481 atcttcaaca ccaccgaatt tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt
      541 cacatgcaaa ccgctcaggt cggcattatg ttgaccattt atgcatgggt agtggcgcta
      601 atgtcattgc cttttatgtt aatgaccagc caggttgaac ggcgcaaatt actgatctgc
      661 ctgtttgtgg tgtttattgc cagccatgta ctgtcgtttt tatcgtggag ctttaccgtt
      721 ctggtgatca gtcgcattgg tgtggctttt gcgcatgcga ttttctggtc gattacggcg
      781 tctctggcga tccgtatggc tccggccggg aagcgagcac aggcattgag tttaattgcc
      841 accggtacag cactggcgat ggtcttaggt ttacctctcg ggcgtattgt gggccagtat
      901 ttcggttggc gaatgacctt cttcgcgatt ggtattgcgg cgcttatcac ccttttgtgc
      961 ctgattaagt tacttccctt actgcccagt gagcattccg gttcattgaa aagcctcccg
     1021 ctattgttcc gccgcccggc attgatgagc atttatttgt taactgtggt ggttgtcacc
     1081 gcccattaca cggcatacag ctatatcgag ccttttgtgc aaaacattgc gggattcagc
     1141 gccaactttg ccacggcatt actgttatta ctcggtggtg cgggcattat tggcagcgta
     1201 attttcggta aactgggtaa tcagtatgcg tctgcgttgg taagtacggc gattgcgctg
     1261 ttgctggtgt gcctggcact gctgctacct gcggcgaaca gtgaaataca cctcggggtg
     1321 ctgagtattt tctgggggat cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg
     1381 ctggcgctgg caccagatgc caccgacgtc gcgatggcgc tattctccgg gatatttaat
     1441 attggaatcg gggcgggtgc gttggtaggt aatcaggtga gtctgcactg gtcaatgtcg
     1501 atgattggtt atgtgggcgc ggtgcctgct tttgccgcgt taatttggtc aatcattata
     1561 tttcgccgct ggccagtgac actcgaagaa cagacgcaat agttgaaagg cccattcggg
     1621 ccttttttaa tgatacgttt taatgatttc caggatgccg ttaataataa actgcacacc
     1681 catacatacc agcaggaatc ccatcagacg ggagatcgct tcaatgccac ccttgcccac
     1741 cagccgcata attgcgccgg agctgcgtag gcttccccac aaaataaccg caaccaggaa
     1801 aaagatcagc ggcggcgcaa ccatcagtac ccaatcagcg aaggttgaac tctgacgcac
     1861 tgtggacgcc gagctaataa tcatcgctat ggttcccgga ccggcggtac ttggcattgc
     1921 cagtggtaca aaggcaatat tggcactcgg ttcatcttcc agctcttccg acttgctttt
     1981 cgcctccggt gaatcaatcg ctttctgttg cggaaaaagc atccgaaaac cgataaacgc
     2041 gacgattaag ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg tatccatcac
     2101 cagttgcccg gcgtaatacg ccaccatcat gatggcaaat acgtacaccg aggccatcaa
     2161 cgactgacga ttacgttcgg cactgttcat gttgcctgcc agaccaagaa atagcgcgac
     2221 agttgttaat gggttagcta acggcagcaa caccaccagc cccaggccaa ttgctttaaa
     2281 caaatctaac attggtggtt gttatcctgt gtatctgggt tatcagcgaa aagtataagg
     2341 ggtaaacaag gataaagtgt cactctttag ctagccttac atcgcattga ccaaaacttg
     2401 aaccgattta gcaaaacgtg gcatcggtca attcattcat ttgacttata cttgcctggg
     2461 caatattatc ccctgcaact aattacttgc cagggcaact aatgtgaaaa gtaccagcga
     2521 tctgttcaat gaaattattc cattgggtcg cttaatccat atggttaatc agaagaaaga
     2581 tcgtctgctt aacgagtatc tgtctccgct ggatattacc gcggcacagt ttaaggtgct
     2641 ctgctctatc cgctgcgcgg cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt
     2701 cgacctggga gcactcaccc gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag
     2761 gttgccgaac ccgaatgata agcgcggcgt actggtaaaa cttaccacca gcggcgcggc
     2821 aatatgtgaa caatgccatc aattagttgg ccaggacctg caccaagaat taacaaaaaa
     2881 cctgacggcg gacgaagtgg caacacttga gcatttgctt aagaaagtcc tgccgtaaac
     2941 aaaaaagagg tatgacgatg tccagacgca atactgacgc tattaccatt catagcattt
     3001 tggactggat cgaggacaac ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt
     3061 caggttactc taaatggcac ctgcaacgga tgtttaaaaa agaaaccggt cattcattag
     3121 gccaatacat ccgcagccgt aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg
     3181 agccgatact ctatctggca gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa
     3241 ccttcaaaaa ttactttgat gttccgccgc ataaataccg gatgaccaat atgcagggcg
     3301 aatcgcgctt tttacatcca ttaaatcatt acaacagcta gttgataacg tgacaacgtc
     3361 actgaggcta tcatgaaacc actttcatcc gcaatagcag ctgcgcttat tctcttttcc
     3421 gcgcagggcg ttgcggaaca aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg
     3481 gttgttcccc catcgcagga acaaccaccg tttgatttaa atcacatggg tactggcagt
     3541 gataagtcgg atgcgctcgg cgtgccctat tataatcaac acgctatgta gttattgtca
     3601 ggctctgatc acagagcctg acatctccgt a
//
LOCUS       DAFATA010000210         3560 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFATA010000210 DAFATA010000000
VERSION     DAFATA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.212"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..176)
                     /locus_tag="LGJ20_004747"
     CDS             complement(<1..176)
                     /locus_tag="LGJ20_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBK2388726.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDP"
     gene            264..1157
                     /gene="nadC"
                     /locus_tag="LGJ20_004748"
     CDS             264..1157
                     /gene="nadC"
                     /locus_tag="LGJ20_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBK2388727.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            1360..1800
                     /gene="ppdD"
                     /locus_tag="LGJ20_004749"
     CDS             1360..1800
                     /gene="ppdD"
                     /locus_tag="LGJ20_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBK2388728.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            1810..3195
                     /gene="gspE"
                     /locus_tag="LGJ20_004750"
     CDS             1810..3195
                     /gene="gspE"
                     /locus_tag="LGJ20_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBK2388729.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDDETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            3185..>3560
                     /locus_tag="LGJ20_004751"
     CDS             3185..>3560
                     /locus_tag="LGJ20_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBK2388730.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLY"
BASE COUNT          855 a          965 c          930 g          810 t
ORIGIN      
        1 tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc
       61 ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccgg gcggcaatcg
      121 taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag
      181 gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt
      241 cgttagctat ctggagtttt aacatgccgc ctcgccgcta taaccctgac acccgacgtg
      301 acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg
      361 aagatttagg cggaacagtc gatgccaaca atgatattac ggcaaaactt ttaccggaaa
      421 attctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtcttttgc ggcaaacgct
      481 gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg
      541 atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc ccatcccgcg
      601 tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca ggagttgcca
      661 gtaaggtacg ccactatgtc gaattgctgg aaggcaccaa cacgcagttg ttggatacgc
      721 gcaaaacctt acccggcctg cgttcagctc tgaaatacgc ggttctttgc ggcggcggag
      781 cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat attattgcct
      841 ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcacccggat gcgccagtag
      901 aagtcgaagt agagaatctg gaagaacttg atgaagccct gaaagcagga gccgatatca
      961 tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacggca
     1021 aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgtgaa tttgccgaaa
     1081 cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt
     1141 caatgcgttt tcgctaatag ttgacagatt tatccgctcc atcgcgggcg gataatctcc
     1201 ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg caacgaaata
     1261 aaaaacctcg gaatctgctt tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt
     1321 ttgccaaagt agcgccaact aaatcaagga gcgaaacaga tggacaagca acgcggtttt
     1381 acacttatcg aactgatggt ggttattggc atcattgcca ttttaagcgc cattggtatt
     1441 cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca aacctttgtg
     1501 ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac
     1561 ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg
     1621 gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg
     1681 acaccgggtt gggataacgc aaacggcgtc accggctgga cgcgcaactg caatattcaa
     1741 agtgacagcg cattgcatca agcctgcgaa gatgtcttcc gctttgatga cgccaactaa
     1801 ggagcggcaa tgaatattcc acagctcact gccctgtgtc tgcgttatca tggagtcttg
     1861 ctggatgcca gcgaagaggt ggttcatgtt gcggtagtcg atgcaccttc gcatgagcta
     1921 ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa
     1981 caaatggaag gtcacgccag tcgcacacaa cagacattgc ccgtagctgt tcaggagaag
     2041 catcagccca aagcagagtt gctaactcga acgttacaat ctgcgctgga acaacgcgcg
     2101 tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggc
     2161 gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac cgccagatta
     2221 aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact
     2281 gtcgaactgg caggaaacgc cgtctcattt cgtattgcga ccttaccatg tcggggtggt
     2341 gaaaaggtgg tattaaggtt gttacagcag gtgagccagg cactggatgt caacacgctt
     2401 ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg
     2461 gtgctggtaa ctggccctac aggcagcggc aaaacggtca cgctttatag tgccctgcaa
     2521 acgctgaata ccgctgacat taatatttgt agcgtcgaag atccggttga gatccccata
     2581 gccggactaa accagacgca aatccatccg cgtgccggac tcacctttca gggcgtgttg
     2641 cgtgcgttat tgcgccagga tcctgacgtc atcatgatcg gagagatccg cgatgacgaa
     2701 acagcagaga tcgctattaa agcggcgcaa actggtcacc tggtgttgtc taccctacac
     2761 actaattcca cctgcgaaac gctggtacgt ttacagcaaa tgggggtcgc ccgctggatg
     2821 ctatcatcgg cgcttacgct ggtaatagcc cagcgtctgg tacgcaaact ttgcccacat
     2881 tgtcgccggc agcaagggga gcccatccac attccagaca atgtatggcc atcgccactg
     2941 ccccactggc aggcacccgg ttgtgtacat tgctaccacg gtttttatgg tcgtacggcc
     3001 ttatttgaag ttctgcccat aacgccggtc attcgtcagc ttatttccgc taataccgac
     3061 gttgaatcgc tggaaacgca cgcacgacag gcgggtatgc gtacgctttt tgaaaacggc
     3121 tgcctggccg tggagcaagg cttaaccacc tttgaagagt taatccgcgt actggggatg
     3181 ccgcatggcg agtaagcaac tctggcgctg gcatggcata accggcgacg gcaatgcgca
     3241 agatgggatg ctatgggcag agagccgtgc tttgctgctc atggcactac agcaacagat
     3301 ggttacccca cttagcctga agcgaatcgc catcaattct gcgcagtggc gaggagataa
     3361 aagcgcggaa gtcattcatc aactggcgac gctactcaaa gccgggttaa cgctttctga
     3421 agggctggca ctgctggcgg aacagcatcc cagtaagcaa tggcaagcgt tgctgcaatc
     3481 gctggcgcac gatctcgaac agggcattgc tttttccaat gccttattac cctggtcaga
     3541 ggtatttccg ccactctatc
//
LOCUS       DAFATA010000211         3495 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFATA010000211 DAFATA010000000
VERSION     DAFATA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.213"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..755
                     /locus_tag="LGJ20_004752"
     CDS             <1..755
                     /locus_tag="LGJ20_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2388731.1"
                     /translation="LCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAA
                     TQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQA
                     GVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSAGGKASDLFGEL
                     TGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGT
                     FIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            835..2982
                     /gene="cadA"
                     /locus_tag="LGJ20_004753"
     CDS             835..2982
                     /gene="cadA"
                     /locus_tag="LGJ20_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBK2388732.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3041..>3495
                     /gene="dtpC"
                     /locus_tag="LGJ20_004754"
     CDS             3041..>3495
                     /gene="dtpC"
                     /locus_tag="LGJ20_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBK2388733.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIA"
BASE COUNT          855 a          882 c          830 g          928 t
ORIGIN      
        1 tgctctgcct gtgggccttc gtgggtgttg aatccgcagc agtaagtact ggtatggtta
       61 aaaacccgaa acgtaccgtt ccgctggcaa ccatgctggg tactggttta gcaggtattg
      121 tttacatcgc tgcgactcag gtgctttccg gtatgtatcc atcttctgta atggcagctt
      181 ccggtgctcc gtttgcaatc agtgcttcaa ccatcctcgg taactgggct gcgccgctgg
      241 tttctgcatt caccgccttt gcgtgcctga cttctctggg ctcctggatg atgttggtag
      301 gccaggcagg tgtacgtgcc gctaacgacg gtaacttccc gaaagtttat ggtgaagtcg
      361 acagcaacgg tattccgaaa aaaggtctgc tgctggctgc agtgaaaatg actgccctga
      421 tgatcctcat cactctgatg aactctgccg gtggtaaagc atctgacctg ttcggtgaac
      481 tgaccggtat cgcagtactg ctgactatgc tgccgtactt ctactcttgc gttgacctga
      541 ttcgttttga aggcgttaac atccgcaact ttgtcagcct gatctgctct gtactgggtt
      601 gcgtgttctg cttcatcgcg ctgatgggcg caagctcctt cgagctggca ggtaccttca
      661 tcgtcagcct gattatcctg atgttctacg ctcgcaaaat gcacgagcgc cagagccact
      721 caatggataa ccacaccgcg tctaacgcac attaattaaa agtattttcc gaggctcctc
      781 ctttcatttt gtcccatgtg ttgggagggg ccttttttac ctggagatat gactatgaac
      841 gttattgcaa tattgaatca catgggggtt tattttaaag aagaacccat ccgtgaactt
      901 catcgcgcgc ttgaacgtct gaacttccag attgtttacc cgaacgaccg tgacgactta
      961 ttaaaactga tcgaaaacaa tgcgcgtctg tgcggcgtta tttttgactg ggataaatat
     1021 aatctcgagc tgtgcgaaga aattagcaaa atgaacgaga acctgccgtt gtacgcgttc
     1081 gctaatacgt attccactct cgatgtaagc ctgaatgacc tgcgtttaca gattagcttc
     1141 tttgaatatg cgctgggtgc tgctgaagat attgctaata agatcaagca gaccactgac
     1201 gaatatatca acactattct gcctccgctg actaaagcac tgtttaaata tgttcgtgaa
     1261 ggtaaatata ctttctgtac tcctggtcac atgggcggta ctgcattcca gaaaagcccg
     1321 gtaggtagcc tgttctatga tttctttggt ccgaatacca tgaaatctga tatttccatt
     1381 tcagtatctg aactgggttc tctgctggat cacagtggtc cacacaaaga agcagaacag
     1441 tatatcgctc gcgtctttaa cgcagaccgc agctacatgg tgaccaacgg tacttccact
     1501 gcgaacaaaa ttgttggcat gtactctgct ccggcaggca gcaccattct gattgaccgt
     1561 aactgccaca aatcgctgac ccacctgatg atgatgagcg atgttacgcc aatctatttc
     1621 cgcccgaccc gtaacgctta cggtattctt ggtggtatcc cacagagtga attccagcac
     1681 gctaccattg ctaagcgcgt gaaagaaaca ccaaacgcaa cctggccggt acatgctgta
     1741 attaccaact ctacctatga tggtctgctg tacaacaccg acttcatcaa gaaaacactg
     1801 gatgtgaaat ccatccactt tgactccgcg tgggtgcctt acaccaactt ctcaccgatt
     1861 tacgaaggta aatgcggtat gagcggtggc cgtgtagaag ggaaagtgat ttacgaaacc
     1921 cagtccactc acaaactgct ggcggcgttc tctcaggctt ccatgatcca cgttaaaggt
     1981 gacgtaaacg aagaaacctt taacgaagcc tacatgatgc acaccaccac ttctccgcac
     2041 tacggtatcg tggcgtccac tgaaaccgct gcggcgatga tgaaaggcaa tgcaggtaaa
     2101 cgtctgatca acggttccat tgaacgtgcg atcaaattcc gtaaagagat caaacgtctg
     2161 agaacggaat ctgatggctg gttctttgat gtatggcagc cggatcatat cgatacgact
     2221 gaatgctggc cgctgcgttc tgacagcacc tggcacggct tcaaaaacat cgataacgag
     2281 cacatgtatc ttgacccgat caaagtcacc ctgctgactc cggggatgga aaaagacggc
     2341 accatgagcg actttggtat tccggccagc atcgtggcga aatacctcga cgaacatggc
     2401 atcgttgttg agaaaaccgg tccgtataac ctgctgttcc tgttcagcat cggtatcgat
     2461 aagaccaaag cactgagcct gctgcgtgct ctgactgact tcaaacgtgc gttcgacctg
     2521 aacctgcgtg tgaaaaacat gctgccgtct ctgtatcgtg aagatcctga attctatgaa
     2581 aacatgcgta ttcaggaact ggctcagaat atccacaaac tgattgttca ccacaatctg
     2641 ccggatctga tgtaccgcgc atttgaagtg ctgccgacga tggtaatgac tccgtatgct
     2701 gcattccaga aagagctgca cggtatgacc gaagaagttt acctcgacga aatggtaggt
     2761 cgtattaacg ccaatatgat ccttccgtat ccgccgggag ttcctctggt aatgccgggt
     2821 gaaatgatca ccgaagaaag ccgtccggtt ctggagttcc tgcagatgct gtgtgaaatc
     2881 ggcgctcact atccgggctt tgaaaccgat attcacggtg cataccgtca ggctgatggc
     2941 cgctataccg ttaaggtatt gaaagaagaa agcaaaaaat aattagctcg tacaagggaa
     3001 gtggcttgcc acttcccttt tttgctcata aggagaacac atgaaaacac cctcacagcc
     3061 gcgcgcgata tactatatcg tggcgatcca aatctgggag tacttcagtt tttacggcat
     3121 gcgtgcctta ctcattctct atctcaccca tcagcttggt tttgatgata accatgccat
     3181 cagcctgttc agcgcatatg cttctctggt ttacgttacc cctattctcg gcggctggct
     3241 tgccgaccgc ctgctcggca accgcactgc agtgattgcc ggcgcgctgt taatgaccct
     3301 tggccatgtg gtgctgggca ttgatacaaa ttcaaccttt agcctgtatc tggcgctggc
     3361 aatcattatt tgtggctacg gtttattcaa atcaaacatc agctgtttgc ttggcgagct
     3421 ctacgacgag aacgatcatc gacgtgatgg cggtttttcg ctgctgtatg ctgcaggcaa
     3481 tatcggttct atcgc
//
LOCUS       DAFATA010000212         3464 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFATA010000212 DAFATA010000000
VERSION     DAFATA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.214"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(318..1637)
                     /locus_tag="LGJ20_004755"
     CDS             complement(318..1637)
                     /locus_tag="LGJ20_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBK2388734.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLNHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1768..3303)
                     /gene="gadC"
                     /locus_tag="LGJ20_004756"
     CDS             complement(1768..3303)
                     /gene="gadC"
                     /locus_tag="LGJ20_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBK2388735.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT          927 a          824 c          849 g          864 t
ORIGIN      
        1 agatcgacta actgcaccat attctcctga aatatgaaga tatactgaaa agaaataagc
       61 gacttaggac agttttaatc tacgctactg ttcttcagaa gagtatagcc catcgtaatt
      121 atttttcggt gacagcgaat atcgtatgtt ttttcatatt catacatttt tattagggga
      181 tttatggctg tttaactaag tgtggttaat tgcgtggtta atttgacttt agtaagcatg
      241 attagtagtg ggatagttca agagggtaac aagccggtgg gtaaagcacc ggcttgttac
      301 aaagtaagaa tgggagttta actgccccaa cgactttgca gatagctgac cgcttgttga
      361 gtctgcggtt tattcagata gtcctcacgg aacaagatgg tgccgctaat ttcgggtacc
      421 gcatcgttaa gatcgagctg ctttttcagt tcgggtacgc cgccgttaat catccagtct
      481 ggctctatct ttgaaggttc acccacttta tagaaggcga taccgatata caggcgggtc
      541 ctggtcggtt taacgacatc cgcccaccat tttgccaaca cgtcataacg cgcggcactc
      601 cgtgagaacg gccagtaaat ttggggagca atataatcca gcaatccttg ttccacccat
      661 cgacgggtgt cagcgtagga ttcgtcatag gctgccgcac ctcgggtatc ggaaccgagc
      721 ggatcgtgtg atcggttacg ccacacgcct gccgggctaa cgccaaattc gactcccggc
      781 ttaatgcttt taatggtgtg cgatactttt gcaattaact gctgagtatt gttgcgccgc
      841 cagtctgctt ttgacgcaaa tgcgcctccg tatttgcggt acgtttcgtt atcatttagc
      901 cgtgaacccg gtgactccgt atagaaatag tcgtcaaact gcacgccatc taccggatag
      961 cgggaaacca cttctgcgac tattgatgtg atccagtcct gaacctcagg gatgcccggg
     1021 tcgaggacaa agcgatcgcc agacgttctg atccagtcac ggtgttgcac atagacgctc
     1081 gccggttgtt gagacagagt gctattcagt tccctgatag taccgggctt cgtattaacc
     1141 gatacgcgat aggggttaaa ccaggcgtgt actttcatcc cacgcttgtg ggcttcgtcg
     1201 agcataaatt gcagcggatc gtaacccgga ttttcaccaa tcttaccggt cataagatcg
     1261 gaccacggca aaattttcga tggccacagg gcggtaccgt ccggcttgac ctggaaaaaa
     1321 accgtgttta taccgagacg ttgcagatga ttcagtttgt cgatcatcgc ctgttgttgt
     1381 acacgggccc ggctggtggg gttactaatg ttaaccgagg aaaccggtgg ccagtcgagg
     1441 cgagaaaccg tggccagcca gatgccacgc atcggttgtg acgattgttg cgtcgtggct
     1501 ggtggctttg aacctgctgg tggtgtcacc atggactctg gaggcgtgct tttacaacta
     1561 cacagtaaaa gtgccagtgc aactagtatc gctggtcttc taatcgttaa tttcttgttt
     1621 cgggagcaga tatccatatg tgctggtttc cggtaaacag atgtgcgctc attctcgtac
     1681 ttatcccggt taagtcaata cgacagcaag cacgaaaaag ggagcgatga cttatcgctc
     1741 ccttgtctta taaccattca gacatggtta gtgtttcttg tcattcatca caatatagtg
     1801 tggtgaacgt gcacgcgggt gcaggaagaa gtgacctttt ggtgcgttct gactgttgat
     1861 tggctccaga gtgacgccgg tattcgcttt gcctttacga tcatgaacag catagagaat
     1921 aaagggcagg gcaagtacca ccaggaaact aacaaccagt aattcaacat acatatcggt
     1981 agaatcaccc tggatgttat ccggcggcag gaaggaaaca ataaacgcca taattgaagt
     2041 cagcagaccg acaattgcca cgaccagttt caccccttta ccaccaggga tattaaatgt
     2101 gcgttttaag tcaggatgtt taagaaccaa cacaatgtag ccaataaaca gcatgaaata
     2161 agcacacaga taaatcacca ccgtcagcgc cagagcgatc aggaaggaca tgttgttacc
     2221 gccaccggta ttggtgagga tgatcaacgc gatagaagta atcaccagct gcgaaatgac
     2281 cagcgttacc ggtacgccat ttttgttcat tttagcgaat gccgctggca gcaggttttt
     2341 ctgcgctgtg acatacatcc cgcgagaagg accaacaatc caggaggcga tttccgccaa
     2401 aacacccagc aacagcagtg cggagatcac gcgaaccgtc cactcaattt ccggtgccac
     2461 atgggacatc agaacggtaa aggtttgcat tacacctgcg gagaggttga tttcattacc
     2521 cggaatgacc atcgcaatag acaaaccgcc aacagagctt aagcagattg ccgccaccat
     2581 cagcagtaac atagccagtg gatagtcgcg cccggggttg ctcatttcat tgacgtgagt
     2641 tgcggaagct tctacgccca tataactcaa aatgaaggca acaaatacaa ccagtgtgcc
     2701 cactttagag aagtcaggga agaaggtctt cgaatccatt tcgatagcaa cgggggcacc
     2761 ggagtgcaga taaatagccg ctaatgcgat caaaataaat gcaggtaaca ggataccggc
     2821 gaagaagcca actttagcaa ttcgcgccgt gtatttcgtg ccaccaaact gcgttaatgc
     2881 cagcgcccaa agaatgatga gtgctgcaat agttttggta atggggtctt cattcagcgc
     2941 tggccatttc aggatgtagg agagtgcccc taacacgaaa tagagcatcg gaataaaacc
     3001 aatggcgatt tgcagatagc caaatgagat cgctgcaaat ccccatctcg gccccagagt
     3061 atttgatacc caggcgaaga caccaccttc ttcccagccg tcgacggtgg ccatttccgc
     3121 agcacaaagt cccacgggaa taaaccataa aatcccgcct aatagcagga agaagactaa
     3181 tgaaaagccc gatgttgcga aggtagggta ttcataaaca gccattacca tcgatgccgt
     3241 tatggcaaag aatccgagta atgtgagctg cttagcttta cctgtctgta ctgatgtagc
     3301 catattatcc ccctaaaacg gtattcctgt cggaaccgca cctgttttgt tctgaagcgt
     3361 attcagaaca atattttccg ttgctaatgc cagtgaacag actttggaaa ttgtcccgaa
     3421 acgggttcgt ttcgggacac cgttaccgtt aaacgttatc aggt
//
LOCUS       DAFATA010000213         3373 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFATA010000213 DAFATA010000000
VERSION     DAFATA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.215"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            1..1642
                     /locus_tag="LGJ20_004757"
                     /pseudo
     CDS             1..1642
                     /locus_tag="LGJ20_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            1626..2108
                     /locus_tag="LGJ20_004758"
     CDS             1626..2108
                     /locus_tag="LGJ20_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388736.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSV
                     EIYLSEKNIIYKIINDQKISRGNGHFISIMVNNYRTHCGVVDINLNFFNDILYSVRLK
                     NISKLENMEFCAKKQRVYFSDKNKKASYKIINYGDYYDVDYYDNNLKNEVFDWIGKWS
                     "
     gene            complement(2023..2208)
                     /locus_tag="LGJ20_004759"
     CDS             complement(2023..2208)
                     /locus_tag="LGJ20_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388737.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            2289..3373
                     /locus_tag="LGJ20_004760"
                     /pseudo
     CDS             2289..3373
                     /locus_tag="LGJ20_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1065 a          608 c          900 g          800 t
ORIGIN      
        1 gacaggctga ctgaacagcg cggttttgac ggccggacgc aacgttacca gtatgacctg
       61 accggaaaac tcacgcagag tgaagatgag gggcttatca ccctctggca ttacgatgcg
      121 tcggaccgta tcacccggcg cacggtgaac ggtgaaccgg cggagcagtg gcagtatgac
      181 gaccacggct ggctgacaga aatcagccac ctgagtgaag gccaccgtgt ggcggtccac
      241 tacggctatg acgataaagg ccgtctgacg ggcgaacggc agactgacgg agaagacgga
      301 ccgcatcccg acgggtgtga tacggacgga cgacgagcgg acccaccact accactacga
      361 cagccagcac cgcctggtgt tccacacgcg gatacagcat ggcgagccac tggtcgagag
      421 ccgctacctc tacgacccgc tgggacggcg aatggcgaaa cgggtatggc ggcgggagcg
      481 tgacctgacg gggtggatgt cgctgtcgcg taaaccggag gagacatggt acggctggga
      541 cggcgacagg ctgacgacgg tacagaccga caccacgcgt atccagacgg tataccagcc
      601 ggggagcttc gcgccgctca tccgcatcga aacagacaac ggtgaacggg agaaagcgca
      661 gtgccgcagc ctggcggaga agatacagca ggaagggagc gaggacgggc acggcgtggt
      721 atttccggct gaactggtgg ggctgctgga caggctggag ggggaaatcc gggcaaactg
      781 cgtgagcagt gaaagccgcc agtggcttgc gcagtgcggg ctgacggtgg agcggctggc
      841 ggcacagata gagccggttt atttgcctga gcgaaaaata catctttatc actgtgacca
      901 ccggggcctg ccgctggcgc ttatcagtga agacggcaat acggcgtgga gcgcggaata
      961 tgatgaatgg ggcaaccagc ttaatgagga gaacccgcat cacctgcacc agccgtaccg
     1021 gctgccgggg cagcagtatg ataaggagtc ggggctgtac tataaccgtc accggtacta
     1081 cgatccgttg caggggcggt atatcacccc ggacccgatt gggttgagag gtggatggaa
     1141 tatgtatcag tatccgttga atcccataca agtgatagac ccaatggggt tagatgcgat
     1201 tgagaatatg acatcaggtg gactaattta tgccgtatct ggtgtacctg gattgattgc
     1261 tgcaaacagc attactaaca gtgcttacca gttcggttat gatatggatg ctattgttgg
     1321 cggagctcat aatggggccg ccgatgcaat gagacattgt tacttgatgt gtcgaatgac
     1381 taagacattt ggatcaacaa tagctgacgt gataggtaaa aatcatgagg cggcagggaa
     1441 tagacaaggt cagccagcta aagaaagaat catggatctt aaaaataaca ctgtcggtat
     1501 tgcttgtggc gatttttctg ccaaatgtag cgatgcatgt attgaaaaat ataacactgg
     1561 gcaactcttc gggttagatg gtataaaagc agataatcca ataaaagcaa agcaagggag
     1621 ttcagatgct tcaaattatt agaggcaaac ttgtcatttt tttaattacc ctttgtttat
     1681 ttgttgttta ccttgggttt gataacaatt caaattctga catcgtattt tatggacata
     1741 aaacaccaaa gagcgttgag atatatcttt ctgaaaaaaa tattatttat aaaataatta
     1801 atgaccaaaa aattagtaga gggaatggtc attttataag tataatggtt aataattaca
     1861 ggacgcactg tggagtggtt gatataaatc ttaatttttt caatgatatt ctttatagcg
     1921 tgcgattaaa aaacattagt aaactggaga atatggagtt ctgcgctaaa aaacaacggg
     1981 tgtattttag tgataaaaac aagaaggcta gttataaaat aattaattat ggagactatt
     2041 acgatgttga ttattatgat aacaatttga aaaatgaagt ttttgactgg attggtaaat
     2101 ggtcataatg aacaacacca gaaagagaaa aatataaaat acatacagaa agacatgaga
     2161 gcaagggacg gggcagggca agatcatgaa aattgtgatg taaatcacga ttttcatctt
     2221 ggctttaacg cctacaggtg atcaaaattt aacctttgga ataactaaaa agataaaaaa
     2281 ggacccaggt gagtattcaa agtttgcttg attatatttc agtgatccct gatatacgac
     2341 aacaaggaaa ggttaaacat aaattatctg ctattttgtt tctcaccgta tgtgcagtaa
     2401 ttgcaggtgc cgatgagtgg caggaaattg aagattttgg acatgaaaga cttgaatggc
     2461 taaagaaata tggtgatttt gataatggca ttccggtcga tgacaccatt gcacgcgttg
     2521 tgagtaacat tgacagtttg gcctttgaaa agatgtttat tgaatggatg caggagtgcc
     2581 atgaaatcac tgatggtgaa attatagcaa tagatggaaa gaccataaga ggctcctttg
     2641 ataagggaaa aagaaaagga gcaatccata tggtgagtgc attctcgaac gaaaatggtg
     2701 ttgtactggg gcaggtgaaa acggaagcca aaagtaatga gattacagcc attccagagt
     2761 tgcttaacct actgtattta aagaaaaatt tgataaccat tgatgctatg ggctgtcaga
     2821 aagatatcgc ttcgaagatc aaagataaaa aagcagatta tcttctggca gtaaaaggca
     2881 atcaggggaa attacatcat gcattcgagg aaaaatttcc tgtaaatgtg ttttctaatt
     2941 ataaaggcga ttcgtttagt acgcaggaga taagtcatgg aagaaaagaa acacgtttgc
     3001 atattgtcag taacgtaacg cctgaacttt tgtgattttg aattcgaatg gaagggatta
     3061 aaaaagcttt gtgtagcatt gtcattcagg cagaagaaag aagataaatc agcagaaggt
     3121 gtaagcatcc gatattatat ttcatcaaag gatatggatg ctaaagaatt tgcacatgct
     3181 atcagagcgc actggctgat cgagcacagt cttcattggg tgttagatgt aaaaatgaat
     3241 gaagatgcca gccggataag aagaggaaac gcagcctaaa taatatctgg aataaagaag
     3301 atggcactga atttattaag agattgcaaa gacattaagg gtgaagagga aaagaaagaa
     3361 ggctgcgtta aac
//
LOCUS       DAFATA010000214         3286 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFATA010000214 DAFATA010000000
VERSION     DAFATA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.216"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(164..240)
                     /locus_tag="LGJ20_004761"
     tRNA            complement(164..240)
                     /locus_tag="LGJ20_004761"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Arg,seq:acg)
     gene            complement(438..514)
                     /locus_tag="LGJ20_004762"
     tRNA            complement(438..514)
                     /locus_tag="LGJ20_004762"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Arg,seq:acg)
     gene            complement(518..610)
                     /locus_tag="LGJ20_004763"
     tRNA            complement(518..610)
                     /locus_tag="LGJ20_004763"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(574..576),aa:Ser,seq:gct)
     gene            complement(926..1111)
                     /gene="csrA"
                     /locus_tag="LGJ20_004764"
     CDS             complement(926..1111)
                     /gene="csrA"
                     /locus_tag="LGJ20_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBK2388738.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1346..>3286)
                     /gene="alaS"
                     /locus_tag="LGJ20_004765"
     CDS             complement(1346..>3286)
                     /gene="alaS"
                     /locus_tag="LGJ20_004765"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK2388739.1"
                     /translation="PKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGAT
                     DLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFY
                     KLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTL
                     DGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNA
                     MIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQV
                     GDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNH
                     SATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRR
                     NLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLF
                     RIISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLER
                     TRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQ
                     LGSTIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGG
                     TDAAALPAALASVKGWVSAKLQ"
BASE COUNT          791 a          928 c          760 g          807 t
ORIGIN      
        1 cgctactaaa gccatatcaa gtaagagatg gtgcatccgg gaggattact cgccttgcgg
       61 ctcgcccttc gggccgttgc taaagcaacg ttatcctccc tggtgcttgc ggttagtacc
      121 gccacctctt cgataccttc atcgctgaag attacggaga atatggtgca tccgtgagga
      181 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
      241 atcgggaaac ttactttact gcagattttt tgataccgct actaaagccg tatcaagtaa
      301 gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc cgttgctaaa
      361 gcaacgttat cctccctggt gcctgcggtt ataccgccac ctcttccata ccttcatcgc
      421 tgaaagtacg tagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
      481 agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa
      541 ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc agcctctcgg
      601 ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc
      661 tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc ctacactttt
      721 atcgtttgca cacttcacgt tcaattagtc tgtaaaaacg tcaaaaagag tgttttatca
      781 acagaaaaat ggaggtctga cagatagtag taatgcaaaa aaatggagac ttaagttgaa
      841 tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg gtgcgtctca
      901 ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt tcagcctgga
      961 tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt acgccaatac
     1021 gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca atcatgaggg
     1081 tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat taaaagagtc
     1141 gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag caatgacaaa
     1201 cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt agccgatata
     1261 cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag
     1321 tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag cctttcacac
     1381 tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac
     1441 gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc cctgctttca
     1501 cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct tcggctaccg
     1561 ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc atggtacgca
     1621 acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca ttaacatcaa
     1681 ttgccttact ggaaagattt gcgctttcct gtgcggcagc ttgttcttta agctgttgta
     1741 gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc gccagattat
     1801 tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg
     1861 tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca gcagtacccg
     1921 attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga gtaccgccac
     1981 acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca tacttctcgc
     2041 cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg ttggtttcga
     2101 tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga atctcttccg
     2161 gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta accagtgaac
     2221 ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg
     2281 cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt
     2341 cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc tggccgtatt
     2401 tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct ttatcaccaa
     2461 cctgaccgcc ggattccgca tagaatggtg tttgatccag cacgaccaca gcttcctggc
     2521 ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact ttgccgttca
     2581 gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc attgcgttgt
     2641 aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc attgctgctt
     2701 caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa
     2761 ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc gtatcaccag
     2821 aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta cgggcaaact
     2881 gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac
     2941 ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag gtttctttcg
     3001 cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt acataaccac
     3061 ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt
     3121 cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact ttcgctaccg
     3181 cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt tgcagcaccg
     3241 cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcgg
//
LOCUS       DAFATA010000215         3204 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFATA010000215 DAFATA010000000
VERSION     DAFATA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.217"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..2226
                     /locus_tag="LGJ20_004766"
     CDS             <1..2226
                     /locus_tag="LGJ20_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2388740.1"
                     /translation="DRLTEQRGFDGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRI
                     TRRTVNGDPAEQWQYDEHGWLTTISHTSDGHRVSVHYGYDDKGRLTGERQTVENPETG
                     EMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLH
                     RETVRSFGSMAGSNAAYELTSTYTLTGQLQSRHLNLPQLDRDYDWNDNGQLIRISGPQ
                     ESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIA
                     EDAHYVYRHDEYGRLAEKTDLIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQV
                     ESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQT
                     VYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAG
                     AVSAESEAWLAQCGLTVEQMESQMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWC
                     GEYDEWGNQLNEENPHHLYQPYRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGL
                     KGGINLYTYPLAPIRYTDPLGLERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDP
                     ETGDSMTFDSSNRIDRRSQRGAGDPYTGEVVGCETNESGISAAYGTTKIYTTDTRARW
                     LHGGGSSLRDPYAPRQGWKPTMGCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKT
                     R"
     gene            2204..2596
                     /locus_tag="LGJ20_004767"
     CDS             2204..2596
                     /locus_tag="LGJ20_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388741.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGGAVSFIFNEETLYFTVFGGDFIMDDLKKVKNT
                     EAWVWYKNCDSKNKNVAVELLQIYELHQ"
     gene            2775..3204
                     /locus_tag="LGJ20_004768"
                     /pseudo
     CDS             2775..3204
                     /locus_tag="LGJ20_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          875 a          695 c          948 g          686 t
ORIGIN      
        1 gacaggctga cggagcagcg tggctttgat ggcaggacgc aacgttacca ttatgacttg
       61 accggaaaac tcacgcagag tgaagatgag gggcttatca ccctctggca ttacgatgcg
      121 tcagaccgta tcacccggcg cacggtgaac ggcgacccgg cggaacagtg gcagtatgat
      181 gaacacgggt ggctaaccac catcagccat accagtgacg gccaccgagt gtcggtccac
      241 tacggctatg acgataaagg ccgcctgacg ggcgaacggc agacggtgga gaacccggag
      301 acgggggaga tgctgtggga gcatgagacg gggcatgcgt acagcgaaca ggggctggcg
      361 acccgtcagg agccggacgg tctgccgccg gtggagtggc tgacgtatgg cagcggttat
      421 cttgcgggga tgaagctggg cggaacaccg ctggtcgagt acacgcggga ccggctgcac
      481 cgtgagacgg tgcgcagctt cggcagcatg gcaggcagta atgcagcata tgaactgacc
      541 agcacataca ccctcacagg gcagctacag agcaggcacc tgaacctccc gcagcttgac
      601 cgggactacg actggaacga caacggacag ctaatccgca tcagcggccc gcaggagagc
      661 cgggagtacc gttacagtga cacgggaagg ctgacgggcg tccacaccac ggcggcgaac
      721 ctggatatcg atatcccgta tgcgacggac ccggcaggaa accggctgcc ggacccggaa
      781 ctgcacccgg acagcacgct cacggcgtgg ccggataacc gcatcgcgga agatgcgcac
      841 tatgtctacc gccacgatga atacggcagg ctggcggaga agacggacct catcccggaa
      901 ggggttatcc ggatgcacga cgagcggacc caccactacc attacgacag ccagcaccgt
      961 ctggtgttct acacgcggat acagcacggc gaaccacagg tggagagccg gtacctctat
     1021 gacccgctgg gccgccggac gggaaaacgg gtatggcggc gggagcgtga cctgacgggg
     1081 tggatgtcgc tgtcgcgtaa accggaggag acctggtacg ggtgggacgg tgacaggctg
     1141 accactgtac agacccaaca gacaagaatc cagacggtat accagccggg aagcttcacg
     1201 ccgctcctga gaatcgaaac agagaatggc gaacaggcga aggcgcggca ccggagcctg
     1261 gcggaggtgt tgcaggagga cacgggggtg acgcttccgg cggagctggc ggtgatgctg
     1321 gggaggctgg agcgggaact gcgtgcggga gcggtgagtg cggagagtga ggcatggctt
     1381 gcgcagtgcg ggctgacggt ggagcagatg gaaagccaga tggaggcgga atacatcccg
     1441 gagcggaggc tgcatcttta ccactgtgac caccggggac tgccgcaggc gctcatcagc
     1501 ccggaagggg aaacggcgtg gtgcggggag tatgacgagt gggggaacca gcttaatgag
     1561 gagaacccgc atcacctgta ccagccgtac cggctgccgg ggcagcagta tgatgaggag
     1621 tcgggactgt attacaaccg tcaccggtac tacgatccgt tgcaggggcg gtatatcacc
     1681 caggatccga tagggttgaa ggggggaata aatctataca cgtatccact ggcaccaata
     1741 cgttacactg atccactggg gttagaacga gttataagtg tatatgggcc tcccgctcca
     1801 gaccgggcag gtgctgaaac tcctcttgtt cttactgata tgactggagg agtgactatt
     1861 tattatgatc ctgaaacagg tgattcaatg acttttgata gtagcaatag aattgatcgt
     1921 cgttctcaac gtggtgctgg tgatccatat acaggtgaag tcgttggttg tgagacaaat
     1981 gaatcaggaa tatcggcagc atatggcaca acgaagatct atactacaga tactagagca
     2041 agatggctac atggtggggg atcatcgttg cgcgatcctt atgctcctcg acaaggctgg
     2101 aaaccaacta tgggatgtac ccgtgcacag aatgaagatg tagatgagtt gtgtaagaaa
     2161 gtgacaagtt ggatgtattc gcatcctgga gagagaatta gatatgaacg ttttaagaca
     2221 cgttaatgcg attaacatgg gagtgtgcat tctaattttg gttattggat gtgcaaatgc
     2281 taaagttctt aaacgatgta tattacccgc tcctgatggt ggcgtagaaa aactatcaaa
     2341 acccaactta catggagtaa ttaaaaaagt tgatttgaac acaaatgaag cggaaattaa
     2401 aataaaagag gggggggcag tgagttttat ttttaatgag gaaacattgt attttactgt
     2461 ttttggtggg gattttatta tggatgattt aaaaaaagta aaaaatacgg aagcatgggt
     2521 atggtataaa aattgcgata gtaaaaataa aaatgtggca gttgaacttc ttcaaatcta
     2581 cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc aggaaaagca
     2641 tcacgtgatg ctgatttttt gcactaaata attcgcattt tatgtttaaa aattgagata
     2701 ttccttatta cctaaagctg ttttttattg cttatacatg atcaaatact ccttacataa
     2761 ttaaggagaa caaaatggaa cttaaaaaat tgatggaaca tatttctgtt atccccgatt
     2821 acagacaagc ctggaaagtg gaacataaat tatcggatat tctactgttg actatttgtg
     2881 ccgttatttc tggtgcagaa ggctgggaag atatagagga ttttggggaa acacatcccg
     2941 atgttttgaa gtgatatggt gattttgaaa atggtattcc tgttcacgat actattgcca
     3001 gagttgtatc ctgtatcagt cctgcaaaat ttcatgagtg ctttattaac tggatgcgtg
     3061 actgccattc ttcagatgat aaagacgtca tcgcaattga tggaaaaacg ctccggcact
     3121 cttatgacaa gagtcgccgc aggggagcga ttcatgtcat tagtgtgttc tcaacaatgc
     3181 acagtctggt catcggacag atca
//
LOCUS       DAFATA010000216         3160 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFATA010000216 DAFATA010000000
VERSION     DAFATA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.218"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..445)
                     /locus_tag="LGJ20_004769"
     CDS             complement(<1..445)
                     /locus_tag="LGJ20_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="involved in the transport of lipid A across the
                     inner membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HBK2388742.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVR"
     gene            complement(482..2746)
                     /locus_tag="LGJ20_004770"
     CDS             complement(482..2746)
                     /locus_tag="LGJ20_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBK2388743.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(2953..>3160)
                     /gene="ihfB"
                     /locus_tag="LGJ20_004771"
     CDS             complement(2953..>3160)
                     /gene="ihfB"
                     /locus_tag="LGJ20_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBK2388744.1"
                     /translation="KEMLEHMASTLAQGERIEIRGFGSFSLHYRAPRTGRNPKTGDKV
                     ELEGKYVPHFKPGKELRDRANIYG"
BASE COUNT          872 a          825 c          738 g          725 t
ORIGIN      
        1 cacgcacaac agtaatcagt gcgccggaag aagaagaagc aacctgttcg gaatcgtagg
       61 taatacgtga caacagcgta cccgttgact gtttgtcaaa gaatgaaact ggcattccca
      121 tcatgtgacc aaacaggcgg cgacgcatgg tcattaccac ctttcctgat acccaggaga
      181 tacagtagct ggagacatag ctggtgatac cacgtaaaat catcagcccg atcaccacca
      241 gcggcatcca caccagcacg gagcgatctg ttttaccaaa gccatcatca agaagtggct
      301 taaggagcga taacatgaag gtatcgctgg ctgcgttgag gattaacgct acgcccgcca
      361 cgatcagacc cgctttaaaa ggcgcaatgg ttggccacag tcggcggaac gtctgccacg
      421 tagagagatc tttgtcgtta tgcattcaaa aaaccagcat ttgttgaaat agccgcatat
      481 tctacccgtt atccactggc acgccaaacc actgatgata ccaacgaggt aaaatttgct
      541 ctctgaggct gctaatccgc cagccttgcg ctgaaaaatt gaccgtcact tgaccctgat
      601 gtggagtatc aagccattga tatccttgct gttgatagcg atgcttaact ttattagagg
      661 gcaatcgcca tgcgttatag cgcgatgccg atgcgagtgc cacttttcca ttcactcgct
      721 gaattaatgg caacgatgat gaggtattac tgccatggtg aggtacctga agcaatgttg
      781 tctgcacttg ctgccagtaa cggcttagca tcttttgttc agcggggact tcaatatcac
      841 cggttagaag aatgctattc gtcccgtcat caaccttaac cacacaggaa tggttatttc
      901 ctttatcgtt gctagcttgt aaaggccagt gcacgctgaa acgcaatcct tgccattgcc
      961 acgcttcgcc acgcacacag ggctgatgat gttcccagtt taacggactt ctgatccata
     1021 acatcggcca tgtgtgcaat attgagtcca gccctccccg gtgatccaga tgttcatgac
     1081 tcagaataac gccttccggt tcaagattat gccagtggag ccaggggata atcagttgtt
     1141 gcccactatc cccttcgggc caggccagtc ctgtgtcata gagaatcgct ttgccgtttc
     1201 tggctatcac cattgccagc ccttgcccga catcaagcat gtacacctgc cactcgtcag
     1261 gtcgaggttt ttgccacagc ggccagcaca tcagcaagcc tacagccaca cacattgctg
     1321 gcaacgttcg ccaggcgttt aatcgccata ccaccagtaa gaaccatggg gaaaatgata
     1381 gccattgcca acgttcagca atgttgatcc acccttctgg caaactcttt aacccccaga
     1441 aaagtaaagc caaagaccgg tcggcaagaa accataaccc ttgctccagg attaacggcc
     1501 cgcttaaatg cacaaccatc gcggcgagga tcaacggaac cgtgataaat gtcaccaagg
     1561 gaattgctaa tagatttgca ataaacgagg tcagactaat gccatgaaat atgacgattt
     1621 gcacaggcat aagcaggagt gtgattccca gttgcaaatg aatgagggaa acaactgcac
     1681 gcaataccgg cggcagttgc cactcaggac agggaaacca ctggtaccaa aaaatcagtg
     1741 ccgcgaccgc agcggcagag agccataaac tttgcgagag aatggcaaca ggatccatca
     1801 gcaaaattgc cgccagacaa catatccata catcccagcc actccactgt cgcccactta
     1861 acttaagcat tccccacgta gcaagcgcca ccacggtacg caatgcagga ggttgcattc
     1921 ccgtcaacca ggcataaaaa gcagcacagc agatcccacc aattaatggt atttgccagt
     1981 ggatccagcg cccaggcaga aaaatttgtc cactgcgaat gagtccggca gccagcaacg
     2041 ccgcaaaagc gatgtgcaat cccgatatcg ccattaaatg cgccgttcca gtatcgcgca
     2101 tgatattttt gatttctttg gggacggata accgttcccc catacctaaa ccaagaataa
     2161 ccgcattcca cggatagggt tgcagcgttg tttgtagtga cgccagatac tgtgcacgca
     2221 ggctacaatt cggttcaata acacttgcct gcagaaagcg gccggtgagc ggttgatgct
     2281 gggcaatggc ataacgctgg ctatcaaagc cgccatcatt aagttggcca tgaaccgcac
     2341 gaactttgag tttcattgac cattgttgtc cagcacaaac cgcttgcgga agatattcgc
     2401 catacagcac aaggcctggc gcagggaata tacgtcgacc ttgtagatga gtaatttgac
     2461 cgtaatgagt ggtcatgccg tcagttgcag tgatctcaac aattgcatcc tgcgtcgcgc
     2521 cagttaaggt ttctcctgcc cacaaaattt gctttgctga taatatgccc caaacgaaaa
     2581 acagcaacgt cagcgcgata taacggacgg ttttaacagg gataaatgcc agtacgcagg
     2641 cgaagagagt cagaaacgca agggttaatg tcccaggcaa ttggggcaaa atcagcaacg
     2701 gaaaaattcc gcaaattatg catacgccga ctgtcgttat tttcatgccc ccctccctgt
     2761 ttatggaaag tgtgcaactt tgttgagcgg ttgtctggtg ggaattttgc gttccccgag
     2821 gcacattcag aagaatttaa cgaaagggtg aaaactgctc aaaactctgc gaggcagctt
     2881 ccagttcctg attagagaaa tgaaaaaagc acccgacagg tgcttttctc gcgttcaagt
     2941 ttgagtaaaa acttaaccgt aaatattggc gcgatcgcgc agttctttac caggtttaaa
     3001 gtgaggaacg tattttcctt ccagttctac tttatcgcca gtcttcggat tacgtccggt
     3061 acgtggtgcg cggtagtgca aagagaaact gccgaaaccg cggatttcaa tacgctcgcc
     3121 ctgcgcaaga gtcgaggcca tatgctccag catctctttt
//
LOCUS       DAFATA010000217         3138 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFATA010000217 DAFATA010000000
VERSION     DAFATA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.219"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            17..448
                     /locus_tag="LGJ20_004772"
     CDS             17..448
                     /locus_tag="LGJ20_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388745.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLFIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMFETHSLDMNNSVLASPEDL"
     gene            669..1301
                     /locus_tag="LGJ20_004773"
                     /pseudo
     CDS             669..1301
                     /locus_tag="LGJ20_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1346..1822)
                     /locus_tag="LGJ20_004774"
     CDS             complement(1346..1822)
                     /locus_tag="LGJ20_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBK2388746.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIKSLDYWLMQINQMHIPLLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNQKKRQQYAPKYILYIPGLMN"
     gene            complement(1905..2123)
                     /locus_tag="LGJ20_004775"
     CDS             complement(1905..2123)
                     /locus_tag="LGJ20_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388747.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            complement(2291..2752)
                     /locus_tag="LGJ20_004776"
     CDS             complement(2291..2752)
                     /locus_tag="LGJ20_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388748.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          975 a          477 c          558 g         1128 t
ORIGIN      
        1 aaaaaaaaga ataaacatga taaacgatct gaagagtata ttgttaaaat cctcagagga
       61 agttgatgtt tttatcaaaa tttttgaaag ttggagaaaa aaattaccgg caatatcagg
      121 tcctgtaaat ctctttatac ctaccagatt caaagataag tatcttgaag tagagtcata
      181 ttttgtcgac aaatcaatat ggaatgtcca tattacattt catgatgata agcgttttgt
      241 gttttttacc gatcaattta ttgctgaatt tagtccgcag gaatttgtgg ataattgtga
      301 gcaatattta attaataatc attgtttctc ccctgacaag gttaacgaaa tctgcgaaca
      361 agccagacat tatttagttg aaaaaatgtt tgaaacacat tctttggata tgaataattc
      421 tgttttggca agccctgagg acttatagct ccctggctca agtttaatga tttgaatata
      481 atctaattgt aatgttttaa tcatctcatg attttttaat gcctgtggta ttttttacga
      541 aaaaatttta tttttaatta tatttcatta ttatttcatc aatgtattct ttggattttg
      601 cttataataa taatggaagg gatgattata aatgaatgcg ttaaagcatt tacactcagg
      661 atgaatatat gggaaaaatt aaaattgtag tttcagatca gcagccgttt atgattgatg
      721 ggataattgg atttctcgga cattatcctg atttatataa ggttgttggg ggctataaag
      781 atctgaagaa agctatagcg gagtgtaaca aatctacagc ctaaatattt attttaggag
      841 aatttgcagg tggaatgatg gggtccgaac tcgtaaaatg ggttaaatcg cataagatag
      901 atgctcatat tattacattt gtagcaaaga tgccgtatat tgattcaata aaattgcttg
      961 aagcaggtgc gaaaggatgc gtatggaaaa ccagtcaccc ggcgaaacta aatcgtgcta
     1021 ttgattcgat tagtaatggt tacacttatt ttgatagtgt acatatggat tgtgaaaaaa
     1081 tatcttccag gtattcctct gataatcaac ttacaaatcg tgagtcagaa atattacaac
     1141 tcatagctga tggtaaaaca aacaaagaaa tcgctaattt cttgcagtta agcagaaaaa
     1201 aagtcgaaac tcatagactc aatatcatga agaaattaga tgttcacagt ggtatcgagt
     1261 tgatcaaaac agctttacgt atgggtgttt gcactatata aaaaaaagag gctttaggct
     1321 cattttgctg aagccttacg attgtctagt tcattaagcc tggtatatac aatatatatt
     1381 ttggggcata ttgctgtctc tttttttggt tatccattgc aaaacccgca ttataagtcc
     1441 ccaggcattg ccacgttact ccacaacgtg aaaaatggtt atataaaatc tcggttccta
     1501 ttttaatgtt cagacaaggg ttatctaaca aatctcgttc atcttttatt attccacgtt
     1561 tttttaacag gggaatatgc atctggttta tctgcattaa ccaataatct aaactcttta
     1621 tattgttatt cttatctctg ttcttgccta tgctatcctt ttttagattg ctttcaacaa
     1681 gagcgatagc tttaattaaa tttggttcga ttctgaacat tgttcctgct tcattagagc
     1741 aagagttgct ggcgaaagac atttttgtaa taaatataca aaacgtgaag atggttaaga
     1801 atttaacttt tggtgagcgc atattttttc atgtgtgagg attgaatgaa agggaggaga
     1861 tactattata aaagtatctc gttatgattt atttggtcaa tcgtctacca ttcaatttta
     1921 tagtaaggtg agataggatt atcttctgaa tacatatcat tttgaaaagg cgccatccat
     1981 gttgaaacta taaatgcttg tatttgatct gtatcagtag cactggtgtt gatgtgttta
     2041 tctaatgata cgacttcatc acccgttgca ttcgctatat ttgaactttg agataaatta
     2101 ttgataccga ttggattagt cataaagctc ccttttttaa ttgtccaggc caatggtctt
     2161 tacatcatac ttttaactct agagcatttt aaatatcttg caataaggtg aatatcttag
     2221 atatttttat ctctgttgtt taaataacaa gttgcataca tctaaataat aaaattgcgt
     2281 caataaaaat ttatcgcata caaattctcg tcagctgctt taatttcaaa atgttgtcaa
     2341 ttataactcg atggctttca ctctcagttt ctttatagta gagtttaatg ttgtttttga
     2401 gcgggttagg cacatacata tctacacatt cttttatcac aactgctttt cgtcttctgg
     2461 gttcgagttg tagtgatttg tctatacaaa tccttgcctc ttcaattttt tcattatgaa
     2521 agtggactag tgccctatag tagtagagag aggctatatc agttgagtgt atcttagcct
     2581 gttcaaataa gatatgagat acttttgctt gtccagacag accagttatc agtcccataa
     2641 tagctaaaat ttttccatcg acagtggtta tgtctgatac ataatctaaa agctctaatg
     2701 ctttttgagc agcaagttca agttcacttt ttccatgaag tgccaaagac atatggcatt
     2761 ccgctagaag gcaataacat tctgttttta gcgtctgtat atcactttta ttttgtaatc
     2821 catcaaatat agtcatggcc ctgtaaatgc tttcaggggt gaagtcgtaa agttctttct
     2881 ttccggctaa catttcatct gataaataat gattcttata tccttggtcc tgcttagcta
     2941 atcttacaga tcgttctggt ttatgtaccg tttgaaggat attatctata ataaattgtg
     3001 atgtattatg tagttcatca acagggagat gattactggc gatgaggaaa aggtttttag
     3061 cgtcgatcaa ctcaatgtat aaagtgttca ctgcattatt ctggtttatt cttcctgtaa
     3121 cgaaataatc tggcttga
//
LOCUS       DAFATA010000218         3107 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFATA010000218 DAFATA010000000
VERSION     DAFATA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.220"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..236)
                     /gene="tppB"
                     /locus_tag="LGJ20_004777"
                     /gene_synonym="ydgR"
     CDS             complement(<1..236)
                     /gene="tppB"
                     /locus_tag="LGJ20_004777"
                     /gene_synonym="ydgR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="mutations in this gene confer resistance to the
                     toxic peptide alafosfalin in Salmonella typhimurium;
                     member of proton-dependent oligopeptide transport (POT)
                     system family; in Escherichia coli this gene is regulated
                     by OmpR although not via osmoregulation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease"
                     /protein_id="HBK2388749.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLG"
     gene            complement(554..988)
                     /gene="uspA"
                     /locus_tag="LGJ20_004778"
     CDS             complement(554..988)
                     /gene="uspA"
                     /locus_tag="LGJ20_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBK2388750.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            1379..1714
                     /gene="uspB"
                     /locus_tag="LGJ20_004779"
     CDS             1379..1714
                     /gene="uspB"
                     /locus_tag="LGJ20_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBK2388751.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(1785..>3107)
                     /gene="pitA"
                     /locus_tag="LGJ20_004780"
     CDS             complement(1785..>3107)
                     /gene="pitA"
                     /locus_tag="LGJ20_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBK2388752.1"
                     /translation="FNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLLA
                     AIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLIV
                     SPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAI
                     GVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQH
                     PALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSLD
                     QRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVALA
                     LGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLS
                     SSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT          810 a          795 c          784 g          718 t
ORIGIN      
        1 cccagcaggt ggtcgccgac atagccgcca atggaaatga ggccatagac cagcgcagca
       61 aaagcaccaa aagtgacaaa agcctgctct tgcgagaatc caagctgttt aacgaagaaa
      121 accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc gacaaaaaag
      181 atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt attcatattt
      241 attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag
      301 tttacatctg tcagagattc ttaatattga tgcggtgaat agattaatac actattacaa
      361 cagaaaataa ccagctgatc acactaatga tggaaaatat gatggtgtta aatgttgtat
      421 tggcaggaga ttttgattgc gtacgcagat cgtaggccag ataaggggtt tacgctgcat
      481 caggtaacaa ggcgcgccaa aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt
      541 cgtggaggga tgattattct tcttcgtcgc gcagcggaac aatcagcata tcaacgtgaa
      601 cggtgttgat cagctgacgt gcggaagaca tcagtttgct ccagaagtcc tggtggtgac
      661 cacaaaccac cagatccata tcgtatttct tgattgcatc gaccagaacc tggcccaggt
      721 cgccgctgcc gctcagggtt tcagtgattg ggtagcctgc attagtggaa agctcggtca
      781 gcgcatgatg tgtctcttca gagatgcgtt tctgcatatc acccagattc acatcaataa
      841 gcccggtgta taggtcagag tagtttacat ctacgtggat cagagaaact ttcgcattgt
      901 aggggcgagc catagagact gctttctcta ccagaacttt gctttccggg gagagatcga
      961 ctgcgatgag aatgtgttta taagccatag tgttactcct tccataaagt tgtcgatgac
     1021 tggccagcta gcgtttcttg tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg
     1081 ggctaaccca gccgtaaaga ttcagtgtta agaccatcct taccttatag cgacccggat
     1141 gatccgtcaa tccgccttgc ttacccgtta gataaacaaa ttttcgacat gaatatattg
     1201 atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct atagagccgc
     1261 tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact gattggcagc
     1321 gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg gaggaggtat
     1381 gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg
     1441 ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc cattgctcta
     1501 tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca aacaggtgcg
     1561 tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg aatttattcg
     1621 ccgctgtgag cgggtgcgtc ggcagtttat tctgaccagc gcattgtgtg gtctggtggt
     1681 ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat aacgcataaa
     1741 aaaagcgggc cagtaaactg acccgctcgt tttggtgtgt acgattacag gaactgcaag
     1801 gagagccagt acagcccgcc ggaaagcagt accgcagccg gaagggtaaa cacccaggcc
     1861 atcagaatgc tggtcacggt tttacgctgt aagccgccac catctaccac catcgtcccc
     1921 gcgacagaag aggagagtac gtgagtagtg gaaaccggca tcccggtata actcgccagg
     1981 ccgatagaca ctgccgccgt catctgggca gacatcccct gagcgtaggt catgcctttc
     2041 ttaccgattt tctcaccgat agtcgttgcc acacggcgcc agccaatcat cgtaccgata
     2101 cctaacgcca gcgcgaccgc catgatgatc cacaccggtg catactcgat ggtgctaagc
     2161 atgtcagact tcagtttctt caacaggcgc tgatcgtcag cactcacgcc aggcatcttc
     2221 accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg gctacgttga
     2281 tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt caggcggttg
     2341 agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt tgcgccagct
     2401 tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc aggatgctgc
     2461 tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc gtagccagtg
     2521 gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa cataaccaga
     2581 ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat agcggaaagg
     2641 atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt ttcacgctcc
     2701 gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg acgcagcaag
     2761 aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag agaaccgaaa
     2821 atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt catcaacgca
     2881 ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga tgcaggtaaa
     2941 ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga gaacaccatg
     3001 gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat atgcacaatg
     3061 gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaa
//
LOCUS       DAFATA010000219         3025 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFATA010000219 DAFATA010000000
VERSION     DAFATA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.221"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(51..986)
                     /gene="ldtA"
                     /locus_tag="LGJ20_004781"
     CDS             complement(51..986)
                     /gene="ldtA"
                     /locus_tag="LGJ20_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBK2388753.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(1048..2127)
                     /gene="cobT"
                     /locus_tag="LGJ20_004782"
     CDS             complement(1048..2127)
                     /gene="cobT"
                     /locus_tag="LGJ20_004782"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBK2388754.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLTPSHLSAEKGARIALSHLGLEPYLDMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(2139..2882)
                     /gene="cobS"
                     /locus_tag="LGJ20_004783"
     CDS             complement(2139..2882)
                     /gene="cobS"
                     /locus_tag="LGJ20_004783"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBK2388755.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISWLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDCMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(2879..>3025)
                     /locus_tag="LGJ20_004784"
     CDS             complement(2879..>3025)
                     /locus_tag="LGJ20_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBK2388756.1"
                     /translation="TNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIG
                     VKIK"
BASE COUNT          793 a          867 c          680 g          685 t
ORIGIN      
        1 aaaaaagcct gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc
       61 tgtcttgaac cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt
      121 gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc
      181 aatggcactt ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact
      241 tccagccagt tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc
      301 acacgcgtcc caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga
      361 atacagccct gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca
      421 tacaacctgc caatataaat cgcgtacagc cccatgggat tatcgggccc cgcaggaaca
      481 aatgcgggca aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc
      541 caggttggag cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc
      601 ccagcctggc cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa
      661 taaagacgca tctcagcgac gttaacaaca atccctttac gaacagtgtc gggcaaaatc
      721 agttgctgcg gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg
      781 ttcgcttcca gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc
      841 tgggtattgt gatcaggaac agttacagta aacgactgcc ccactaaacg gctaccctct
      901 ggaggtaatg gataagttac cgccaggcta gtatggctgg caaaaagcag agcaaatgag
      961 caaagaatat ttacacgacg catcatgtcc ctttcctatg tcgcgaaagc tatccgttaa
     1021 gtatagcttt tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg
     1081 tagaacaata ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc
     1141 gatgatgggc atcgccagag ctgcgccact cccctcacct aaacgcatct ccatatcgag
     1201 ataaggctcc agccccaaat gcgacagcgc tatacgcgcc cctttttctg ccgacaggtg
     1261 agaaggagtg agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc
     1321 ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac
     1381 gccagccatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg
     1441 cggatttggt tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt
     1501 atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt
     1561 gctgactatt gctgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag
     1621 cgtgacacca ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc
     1681 agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg
     1741 catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac
     1801 gttagcgccc gcttgttctg ccagcacaca cacgccggtt gttccacggg tcatattttc
     1861 agcctgtatg gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg
     1921 atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat tcaaccccgg
     1981 cattcctgcc agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag
     2041 taacccgtcg atatgccgtt gtgcacgcga catagcggca ggatcgatag cagggatcgt
     2101 attcagtaaa tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag
     2161 accagttcac caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta
     2221 cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca
     2281 ccatgcatac ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag
     2341 gtttgtcgcc catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa
     2401 cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc
     2461 gatgcaagga tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt
     2521 acaacaaaaa tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc
     2581 aacatgcaat cgcggctacg tgcagaaaat acgccatcac aagtatcggc gaggccgtcc
     2641 aggtggaatc ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt
     2701 acgccacacc atgcctgcag caccatgaag accagccagc taatcgcgcc aagcaataat
     2761 ccaattaaag gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac
     2821 cagcgacgcg gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc
     2881 atttgatttt tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca
     2941 gctgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca
     3001 caatccccat tcccacttcg ttagt
//
LOCUS       DAFATA010000220         2957 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFATA010000220 DAFATA010000000
VERSION     DAFATA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.222"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(122..271)
                     /locus_tag="LGJ20_004785"
     CDS             complement(122..271)
                     /locus_tag="LGJ20_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK2388757.1"
                     /translation="MHEPPVQSDCCACPGNYRLESNPERHDKAPLAAATGNGLEELSL
                     DVMRL"
     gene            841..990
                     /locus_tag="LGJ20_004786"
     CDS             841..990
                     /locus_tag="LGJ20_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388758.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPV
                     GREAL"
     gene            complement(1863..2228)
                     /locus_tag="LGJ20_004787"
     CDS             complement(1863..2228)
                     /locus_tag="LGJ20_004787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388759.1"
                     /translation="MPPQNRSSSSSSYSSPSVNSGSSSALQIDAAWASFEAMIERRLE
                     EIKPKTPAQIKANGDSRNKTIVISALVIILAIIVIYIGAIYIIDSVTGKEFTSSLMPV
                     ITAIISGVFGYLSGEKSSK"
     gene            complement(2228..2893)
                     /locus_tag="LGJ20_004788"
     CDS             complement(2228..2893)
                     /locus_tag="LGJ20_004788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388760.1"
                     /translation="MVHVMSFQLAIFFKQHITRPDHFFSSINDAAGNLFDRMPQIIPL
                     PDEVPPEIPRVAATNMSGTYNFNISLNRLDFTLNVSDSQFKEPEALSDFVLKSKLIVK
                     NIPNEIEIIRIGIIGNYFELERLPAIALAKKYSKKDLGNISEFNFRYNKISQEFGYSF
                     NNVFSISNAEINTKGIVSDGVFIQKDINNNPSGLSIKKDMAMEIITKKIKELSTSGLE
                     GIS"
BASE COUNT          769 a          646 c          650 g          892 t
ORIGIN      
        1 aaaaaaaacc gccctggcag gcggtttttt agaggttaga aagacctagg aaatctaact
       61 aaccgaggta actggcttag agggagcgca gtcaccaaaa cttgttcttt tagtttagcc
      121 tttacaggcg catcacatca agactcaatt cctctaatcc gttaccagtg gctgctgcca
      181 gtggcgcttt gtcgtgtctt tccgggttgg actcaagacg atagttaccg ggacaggcgc
      241 agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttcc
      301 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
      361 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg
      421 gatctttata gtcctgtcgg gtttcgccgc cactgatttg agcgtcagat ttcgtgatgc
      481 ttgtcagggg ggcggagcct atggaaaaac ggcagcgccg cggccttctc gtttccctgt
      541 taagttcgct cctggcatct acccgaaaat ttccgcccca tttgtaagcc ggtaccgctc
      601 gccgcagccg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatctcctgc
      661 attgcatctt ctgctgacgc gccggtgcca cctttttctc ctgccacatg aagcactttc
      721 ataaagtgtt actccaccgt agcatattga tcccatatac actccgctag tgctacgtga
      781 ctggttcagg gctgcgctcc gaaacccgct aacaacctct ccgctactgg atatcacccc
      841 atgccatctg aatatgatga acaactggat tcgactgata cgcagcttcc cggcgaatat
      901 gacctttttg atttaatggc gcaggttact gaagagaacc tgcacgcccc ggtggatttt
      961 gggccgcctg tcggccggga agcgttatag ctttttcgct tacccgcgta cgtgtaatac
     1021 gccggtatta catcttattt ctttattttc atgtaattac aggtggttgt aatacgttgc
     1081 gtattacata aataaacggg aacttcagtt tttacctttt aattcatgca attgcgatga
     1141 aaatcgcggt tgcgtatggt gtatgaccat tttaaggttg aaaaataatc aaccatggag
     1201 ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa gcgttcctca ctgtatcgtt
     1261 catgcctgca tgaacgggtt attcttcctg actacagaat tacatcaaaa gtacatcaaa
     1321 attacatcac ttttacgtca gatcgcgggt gtacaacaga attacatcaa aagtacatca
     1381 aaagtacatc aaaattacat cacttttaca tcatttttac gtcagatctg acctctgaac
     1441 tattcgtaca tccgcccctc ttcctgggcc tcctgcacgt gctgaaaatg gtttttccgg
     1501 ctgtgccggc aacgcctcac ggccacgcaa taaattgcgc atccgttcac cgttatcggg
     1561 gtggttcgtg gtggctttca gggcgttcat cggattttag ccgctaaaat cccggccagg
     1621 tatgtccgtg catagccatg cagtcgcgcc agaatcatca ggggcgtttt ttctcgatat
     1681 gggcacagaa aggtgtgtgt ttcaggccgg aggcctcaga aacgcgctga gggcctctgc
     1741 ggcggtgcac gcaacggatg ttatggtaaa ttgaagttaa tttcaccaca tgttaataaa
     1801 tttgtagttt tgtgtttggg ataataagtg ctgccatatc ttaatacagc agcgcttatt
     1861 ttttatttgg atgatttctc tccagaaaga tatccaaata cgccacttat tattgcggtt
     1921 attacgggca taagtgagga cgtgaactcc ttcccagtga cagaatcaat tatgtatatt
     1981 gctccaatat atataactat tatagctaat attataacta aagcagatat gacaatggtt
     2041 ttatttctgc tgtcaccatt tgctttgatt tgtgctggcg tcttaggttt tatctcttcc
     2101 agtcttcttt ctatcattgc ttcgaaagaa gcccatgcag catctatttg caaagcagac
     2161 gagctaccag aattcacact tggtgaactg tatgacgagc tggatgatga acggttctgt
     2221 ggaggcatta ggatatcccc tctaagccac ttgttgacaa ctccttgatt ttcttagtta
     2281 ttatttccat tgccatgtct ttttttattg ataatcctga tggattgttg tttatatctt
     2341 tttgtataaa tactccatct gagactattc ccttagtgtt tatctctgca ttgctaatcg
     2401 agaaaacatt attaaaacta tatccgaatt cctgagatat tttattgtat cggaaattaa
     2461 actcacttat atttccaaga tcttttttgg agtatttttt tgctaaggct atagctggaa
     2521 gtctttctaa ttcgaaataa ttgccgatta ttccaatccg gataatctca atttcgttag
     2581 gaatgttttt tacaattagt tttgacttta gtacaaagtc acttaaagct tctggctctt
     2641 taaattgaga atcggataca tttaatgtga aatctaatct gttgagtgat atgttaaagt
     2701 tatatgttcc cgacatattt gttgctgcca ctctaggtat ctctggtggt acctcatctg
     2761 gaagtgggat aatttgaggc atcctatcaa ataaattgcc agcggcatca ttaatactgg
     2821 aaaaaaaatg atccggtctt gttatatgct gcttaaaaaa gatagccaac tggaaactca
     2881 tgacatgtac catttcaact ccatttttat tagtgtcttg actgaagtat aaagcgaaag
     2941 gtaatcttct tacgaag
//
LOCUS       DAFATA010000221         2928 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFATA010000221 DAFATA010000000
VERSION     DAFATA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.223"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..951
                     /locus_tag="LGJ20_004789"
     CDS             <1..951
                     /locus_tag="LGJ20_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388761.1"
                     /translation="QETVNQASGALQKKQNGADIPGKDTFTKNIGACRAYSAWLNIGG
                     DSQVWTTAQFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVIG
                     TRGAMTIRVTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGR
                     RVANDKAVGWNWNSGVYDADISGASTLILHFNMNAGSCPAVQFRVNYRNGGIFYRSAR
                     DGYGFEANWSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLSSVQTWNGPGWSD
                     RSGYVVTGSVNGNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            954..1487
                     /locus_tag="LGJ20_004790"
     CDS             954..1487
                     /locus_tag="LGJ20_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2388762.1"
                     /translation="MMHLKNITAGNPKTVEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWMFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLFAQKV
                     ESTDTFSLPVTFPEQPE"
     gene            complement(1516..2043)
                     /locus_tag="LGJ20_004791"
     CDS             complement(1516..2043)
                     /locus_tag="LGJ20_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2388763.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRRADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2045..2887)
                     /locus_tag="LGJ20_004792"
     CDS             complement(2045..2887)
                     /locus_tag="LGJ20_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388764.1"
                     /translation="MTGELKIGTVNALRIFNDAFGLIFRRSEDFLHFIPTAEGQGENG
                     DIGPLRPFAINLRTGAISVSHGAKIEGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHIYKDIESNGSNFRLKGNYRHHITFANEDGSIRMFLWKD
                     KDGDGVHINNGSSGGGDFVFKTDGGFALGSGAQFASNGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDSGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
BASE COUNT          809 a          669 c          643 g          807 t
ORIGIN      
        1 caggaaacgg taaatcaggc ttctggcgca ttacagaaaa aacagaacgg cgcagatatt
       61 ccgggaaaag ataccttcac caaaaatatt ggggcctgtc gcgcatatag cgcatggctg
      121 aatattggtg gcgatagtca ggtctggaca actgcgcaat ttatttcgtg gctggagagt
      181 cagggggcat ttaaccatcc ttactggatg tgcaaaggct catgggctta tgcaaataat
      241 aaggtcatta cagatacagg ttgcggaaat atttgtcttg caggtgctgt ggtggaagtt
      301 attggcaccc gcggcgcaat gaccatacgc gttaccacgc cgagcacgtc cagcggtggc
      361 ggaatcacta acgctcaatt cacttatatt aatcatggtg atgcttacgc tcctggctgg
      421 cgacgagact acaacacgaa aaaccagcag cctgcatttg ctttagggca aacaggacgc
      481 agggtcgcaa atgataaagc tgttggctgg aactggaata gcggcgttta tgatgcagat
      541 atcagtggcg catcgacatt aatcctccac tttaatatga atgcggggag ttgccctgct
      601 gtacagttcc gcgtgaatta cagaaatggc ggtatctttt atcgttcagc gcgtgatggt
      661 tatggctttg aagctaactg gtcagagttt tacaccacaa cacgcaaacc ctctgcggga
      721 gatgttggtg catatacgca ggcagaatgt aactcaaggt ttattacagg tattcgcctt
      781 ggcggtctgt catctgttca gacatggaat ggtcctggct ggtctgacag gtcaggttat
      841 gtcgttactg gttcagttaa cggaaaccgt gatgaattaa ttgatacaac tcaggcaagg
      901 ccaattcagt attgcattaa tgggacttgg tataacgcgg gaagtattta attatgatgc
      961 acttaaaaaa cattaccgct ggtaatccta aaacagtgga gcagtaccag ctaacaaagc
     1021 aatttaacat taagtggctt tattcagatg atggaaaaaa ctggtatgag gaacaaaaga
     1081 atttccagcc agacactttg aaaatggtct atgaccataa cggcgttatt atttgcattg
     1141 aaaaggatgt ttcagcaatt aatccagagg gggcaagcgt cgttgaatta cctgatatta
     1201 cagcaaatcg ccgggctgat atttcgggga aatggatgtt caaagatggc gtagtgataa
     1261 agcgaactta taccgaggaa gagcagaggc aacaagcgga aaatgaaaag caaagcctgt
     1321 tgcaacttgt cagggataaa acccagctat gggactcaca gctacggctg ggcatcattt
     1381 ccgacgagaa taaacaaaaa ttaacagagt ggatgctctt tgcgcagaaa gtcgaatcta
     1441 cagacacttt cagcctgcca gtaacgtttc ccgaacaacc agaatgagag aaggcccgat
     1501 atcgggcctt aatttttatt caggcttttg tggccattca ggatttgccg tatccacacg
     1561 gctgaccaga acactgtagc gttcccatgc ttccagtcgt gtgcgttcct cgtctgttgc
     1621 catattcagc ctgacagcgc gttccagcgg ctggatgact gattcagctt cggaaagcaa
     1681 tgcggccttt tgtgattcgg cctgttgttg cagttcctgt tctgtatatt ccctctttac
     1741 tactttacca tccagaaaca tccattttcc gttattgtct gcgcgacgat ttgccgtaat
     1801 atcaggaact tcaactatgc ttagcccttc aggattaata gttgaaacgt ctttactgac
     1861 agcaacgatg atgttgttct catcgtaagc aatttttatg gtgtctggct gaaaattctt
     1921 ttgttcctca taccagtttt tgccatcttc acaaaagagc cataccacgc ccgctttttt
     1981 agttagctgg tattgttccg gtgttttcgg attcccggcc ttaatatttt tcaggtgcat
     2041 catcttaaac actccccacg ttataccata ttcccccaat caatttctga attggccttc
     2101 tggcaacagt gtcgacaata tcatccgcat tgctgttaat tgcggctgtt aaaacataac
     2161 ctgacgcatc gccaaagccg gagtcgcgcc atacgttttt atattcaatg ctgccaagac
     2221 gaatatcccg aacgacatga ttatgcagcc atgtgcttaa ccagttgttt tcccacactg
     2281 aaccataaat atcgccatta gacgcaaatt gcgcaccact tcccagcgca aatcccccat
     2341 ctgttttaaa aacgaaatca ccgccaccgc ttgaaccatt attaatatga acaccatcac
     2401 catctttatc tttccacaaa aacatacgaa tacttccgtc ttcgttggcg aatgtaatgt
     2461 ggtggcggta attacctttt aatctaaaat tgctgccatt agattcaata tctttataaa
     2521 tatgtaatcc taactcatca atatgtccga tgcgcgaacc atttgaatag aagctaataa
     2581 taccatctcc atcttgccta aatccggtgt cgttatctcc cagaacaatg gagttttcac
     2641 ccagtgcgtt atcagtagct ccaatagcta gacctccttc aattttggcc ccgtggctga
     2701 cagatatagc acctgttctc agatttatag cgaatggcct taatgggccg atatcaccgt
     2761 tttcgccttg tccttcagcc gtcggaatga aatgaagaaa atcttctgaa cgacggaaaa
     2821 taagaccgaa ggcatcattg aaaattcgca gcgcattcac cgtgccaatt ttcagctctc
     2881 cggtcatttt atcgccggaa cgctgaacgg cgttaccagc cttgttta
//
LOCUS       DAFATA010000222         2829 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFATA010000222 DAFATA010000000
VERSION     DAFATA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.224"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            394..2673
                     /gene="ydeP"
                     /locus_tag="LGJ20_004793"
     CDS             394..2673
                     /gene="ydeP"
                     /locus_tag="LGJ20_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBK2388765.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICHVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          763 a          648 c          685 g          733 t
ORIGIN      
        1 aacaaagttt cagccggaac cgtccagtca gtcattggtg tcacttttat ctatgaataa
       61 ttgtgatttt gaccttagtc tatgcgctcc attcacgaaa attgcgcgga taattatttt
      121 gtgaaagcta ttatcctaca cctgtaagaa aatccgcgca caattgaagc cagagtctta
      181 ttttttatat taaaaaatat gttaatttaa acactatgag agatgatgct taccttatcg
      241 tatttaatct attgtttaac gataatagat taaaaataga aagagagtag tcccctactc
      301 ttatatatcc atgttggcga taatcccttt gtatgtactg tgcatcatcg ctattacaaa
      361 tcctaataat tcatttccac acaggataag tagatgaaga aaaaaattga atcctaccag
      421 ggtgctgccg gtggttgggg tgctgttaaa tccgtagcga atgcagtacg taagcagatg
      481 gatatacgcc aggatgttat tgccatgttt gacatgaata agccagaggg ctttgactgt
      541 ccgggttgtg catggccaga tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc
      601 gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa acgcctcttt ctttgctgag
      661 aatacggttc aatcattact tacctgggga gaccacgagc ttgaggctgc ggggcgactc
      721 actcagcctt tgaaatatga tgccgtcagc gactgttaca agccattaag ctggcaacaa
      781 gctttcgatg aaattggcgc acgccttcaa agctatagtg atcccaatca ggttgaattc
      841 tatacttcgg gccgcacttc caatgaagct gcctttcttt atcagctttt tgcccgtgaa
      901 tacgggagca ataactttcc cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt
      961 ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc tggaagactt tgagaagtgc
     1021 gatttagtca tttgcattgg gcataaccct ggtacaaacc accctcgcat gctgacttcg
     1081 ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca tcaatcctct acaggaacgt
     1141 ggcctggagc gatttactgc accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact
     1201 cagttggcca gtgcctacta taacgtgcgc attggtggtg atatggcgtt gctcaagggg
     1261 atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg ccgcaggtcg gccctcattg
     1321 ctggatgacg aatttattca aacgcatacc gtcggctttg acgagctacg ccgtgacgtt
     1381 ctcaattccg agtggaaaga tatcgaacgt atttctggat taagtcagac tcaaatcgcc
     1441 gaactggctg atgcatatgc cgctgccgaa cgaaccatta tctgttacgg aatggggatc
     1501 actcagcacg aacatggtac ccagaatgta cagcaactgg tcaatctgct gttgatgaaa
     1561 ggtaacattg gcaagcctgg tgcgggtatc tgcccactac gtggccactc taatgtacag
     1621 ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag agtttctggc tcgcctgggt
     1681 gagcgctatg gcttcacacc acctcaagca cctggacatg ctgcaattgc cagcatgcaa
     1741 gcaatatgta cagggcaggc tcgagcattg atctgcatgg ggggcaactt tgcactggca
     1801 atgccagatc gggaagcgag cgctgtaccg ttaacgcaat tagatctggc ggtacacgta
     1861 gccactaagc ttaaccgctc tcatctgctg accgcacggc atagctatat tctgccggtt
     1921 ctgggacgta gcgagattga catgcaaaaa agcggtgcgc aggcggtaac cgttgaggat
     1981 tcaatgtcga tgattcatgc ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa
     2041 tcagagtgtg cagtggtcgc gggaatcgcg caggcagcac taccccagag cgtggtagcc
     2101 tgggagtatc tggtggaaga ttatgatcgc attcgcaatg acattgaagc tgtgctgcca
     2161 gagttcgccg attataacca gcgcatccga catcctggtg gttttcacct gataaacgct
     2221 gctgctgaaa ggcgctggat gactcagtca ggtaaggcta atttcattac cagcaaaggg
     2281 ctgttagaag atccctcttc agcgtttaac agtaagctgg tcatggcgac agtacgcagc
     2341 cacgatcagt acaacacgac gatttatggt atggatgatc gctatcgagg ggtattcggt
     2401 caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccatgt aaaaaacggc
     2461 gaaagagtta atcttattgc gcttacgcca gacggtaagc gcagctcacg ccgcatggat
     2521 agattaaaag tggtcattta ccctatggct gaccgctcac tagtgaccta ttttccagaa
     2581 tcgaatcaca tgctaacact tgataaccac gatccattaa gtggcattcc tggctataaa
     2641 agtattccgg ttgaattaga accatcaaat taatgcctct tctcatttct tctgctgtca
     2701 tccgcacagc agaagaattc ctcattgact attatcgcgc aatttgctca catggattaa
     2761 attaaactac atactataag atataaactt ctgcctacag ctgtaagaaa ctccgctcag
     2821 tactgaagc
//
LOCUS       DAFATA010000223         2642 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFATA010000223 DAFATA010000000
VERSION     DAFATA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.225"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            19..855
                     /locus_tag="LGJ20_004794"
     CDS             19..855
                     /locus_tag="LGJ20_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388766.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFTLSSSNNST
                     KNATFNLWGASTRPVVAELGDEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            859..1386
                     /locus_tag="LGJ20_004795"
     CDS             859..1386
                     /locus_tag="LGJ20_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2388767.1"
                     /translation="MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDGKNWYEELKNF
                     QDDTIKIVYDENNIIVAITKDASTLNPEGFSVVEIPDITANRRADDSGKWMFKDGTVV
                     KRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMATDEERTRLESWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1415..1948)
                     /locus_tag="LGJ20_004796"
     CDS             complement(1415..1948)
                     /locus_tag="LGJ20_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2388768.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1951..2508)
                     /locus_tag="LGJ20_004797"
     CDS             complement(1951..2508)
                     /locus_tag="LGJ20_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388769.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGFELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          752 a          561 c          591 g          738 t
ORIGIN      
        1 aacagggctg gtaacgccgt gcaaaaaaat ggcgatacct tgtctggtgg gcttactttt
       61 gaaaatgact caatccttgc ctggattcga aatactgact gggcgaagat tggatttaaa
      121 aatgatgccg atggtgacac tgattcatac atgtggtttg aaacaggcga caacggcaat
      181 gaatatttca aatggagaag ccgccagagc accacaacaa aagacctgat gacgctgaaa
      241 tgggatgcac taaatattct tgttaatgct gtcattaatg gcagtcttgg agttggttcg
      301 acgaatgcgt taggtggtag ctctattgtt cttggtgata atgataccgg atttaaacag
      361 aatggagacg gtattctgga tgtttatgct aatagtcagc gtgtattccg ctttcagaat
      421 ggagtggcta ttgcttttaa aaatattcag gccggggatg gtaaaaaatt cacgctatcc
      481 agctccaaca actccacaaa gaatgcaacg tttaatttat ggggtgcttc aacccgtcca
      541 gtagttgcag agttaggcga tgaggcagga tggcatttct atagccagcg aaatacagat
      601 aactcggtaa tattttctgt taacggtcag atacagccca gcaactgggg gaattttgat
      661 tcacgctatg taaaagatgt tcgcctgggt acgcgtgttg ttcaattgat ggcgcgtggt
      721 ggtcgttatg aaaaagccgg gcacgcaatt accggattaa gaatcattgg tgaagtagat
      781 ggcgatgatg aagccatctt cagaccaata caaaaataca tcaatggcac atggtataac
      841 gtagcacagg tgtaaattat gcagcattta aaaaatatta agtcaggaaa tccaaaaaca
      901 aaagagcaat atcagctaac aaagaatttt gatgttatct ggttgtggtc agaagatggg
      961 aaaaactggt acgaggaatt aaagaacttt caggacgaca ccataaagat tgtatacgac
     1021 gaaaataata ttattgttgc cataaccaaa gatgcctcaa cgcttaatcc cgaaggcttt
     1081 agtgtcgttg agattccaga tataacagcc aatcgtcgtg ccgatgattc agggaagtgg
     1141 atgtttaagg acggaactgt ggttaagcgg atttatacag cagacgaaca gcaacaacag
     1201 gccgaatcac aaaaggtcgc gttgctttct gaagctgaaa gcgttattca gccactggaa
     1261 cgcgctgtca ggctgaatat ggcgacggat gaggaacgca cacgactgga gtcatgggaa
     1321 cgctacagcg ttctggtcag ccgtgtggat acagcaaatc ccgaatggcc acaaaagcct
     1381 gagtaaaaat taaggcccaa tatcgggcct tctctcattc tggttgttcg ggaaacgtta
     1441 ctggcaggct ggaggtgtct gtagattcga ctttctgcgc atagagcatc cactcggtta
     1501 atttttgttt attctcgtcg gaaatgatgc ccagccgtag ctgtgagtcc catagctggg
     1561 ttttatccct gacaagttgc aacaggcttt gcttttcatt ttccgcttgt tgcctctgct
     1621 cttcctcggt ataagttcgc tttatcacta cgccatcttt gaacaaccat ttccccgaaa
     1681 tatcagcccg gcgatttgct gtaatatcag gtaattcaac gacgcttgcg ccttccggat
     1741 taattgctga aacatccttt tcaatacaaa taataacgcc gttatggtca tagaccattt
     1801 tcaaagtgtc tggctggaaa ttcttttgtt cctcatacca gttttttcca tcatctgaat
     1861 aaagccattt gatgttaaat tgctttgtta gctggtattg ctcttttgtt ttagggttgc
     1921 cagcagtaat gttttttaag tgcatcatcg ttaaatactc cccgcgttat accacgtccc
     1981 attaatgcaa tactgaattg gccttgcctg tgttgtatca attaattcat cacggtttcc
     2041 gttaactgaa cccgtaacga cataacctga cctgtcagac cagccgggac cattccatgt
     2101 ctgaacagat gacagaccgc caaggcgaat acctgtaata aaccttgagt tacattctgc
     2161 ccgcgtatat gcaccaacat ctcccgctga aggttttcgt gtcgtggtat agaaatctga
     2221 ccaacctaat tcaaacccat aaccatcacg agccgacctg taggaaatgc caccattttt
     2281 ataattcaca cggaattgta cggcaggaca gctaccagca tttatattaa agtgaagaat
     2341 taatgcagat gcaccaccaa tcagggcgtt atatgcacca ctatcccaat tccatccaac
     2401 ggatgtatca ccattaacag tattacctgt ttgtcggagt gcaaatgcat tgacattttt
     2461 ggcactaatt gaaatatcgc tagaaccatc aaaaccaact ccagcaattt ttcgtgctgt
     2521 tttcagcttt gttgcagtag cggcattgcc ggacagttcc ccagaaaggc caccgctgaa
     2581 tgtttgtcga ttggtccagg tattcgctgt actgagtaac ggtattttct ccccgcttgt
     2641 gc
//
LOCUS       DAFATA010000224         2533 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFATA010000224 DAFATA010000000
VERSION     DAFATA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.226"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..552)
                     /locus_tag="LGJ20_004798"
     CDS             complement(<1..552)
                     /locus_tag="LGJ20_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2388770.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTT"
     gene            complement(918..2456)
                     /gene="cadC"
                     /locus_tag="LGJ20_004799"
     CDS             complement(918..2456)
                     /gene="cadC"
                     /locus_tag="LGJ20_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HBK2388771.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGNLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          785 a          532 c          518 g          698 t
ORIGIN      
        1 agtggtatcc gcagtattcc agttagctgc ataagttgcc gcatcaaacc aatgccagcc
       61 aacaatagca gtcatcacca caggaataag aaccagcacc agaccaatag tggttaaacg
      121 gcttacccaa gtaccgccga gcatatttac aaaggtaaat acccagacga tagcaataca
      181 ggcgataccc gccggaacag gatcatttaa tactgggaag aaggtggaaa gataagatac
      241 cgcggtaata ccaatcgcca ggttaccaat ccagttagca tggtaataaa gaacacctgt
      301 ctgaaaacca aatgcagggg aaatttctcc ggcataagca attgggccac cttgttgcgg
      361 gttttttgtt gccagtcggg catatacata cgccagcgac attgcaccaa taatagagat
      421 aatccaaccc cagatagcaa taccaccgat acttgctagg ttcgcaggta ataatgcaat
      481 accgctcccc atcatattac cggcaacaac accggtacag gcaaatagcc cgatcttctt
      541 ggcagaactc atgctcttct cctaatttca tttttgaatt tggagtccgg gtcatgatgt
      601 ataactattt cctgaccaga ccaaactggc gctaagaata ctcacgaaaa aaggattaat
      661 cctaaagatt aggtgaaata atacaaaagt ttctgtaagt gagaacttga ggttttttat
      721 taacacatca ggatcgcaag ttgatatcat gaaaagataa acattcaatg tttacaatgg
      781 attgcgggat attctctggt taaatttatg taataaaaat tatgcggcaa ataaattgcc
      841 gcaacatatt ataccaacag aaacatacaa aaactcaaca acaaatattc ccgagcataa
      901 atcaacccgg gagttactta ttctgaagcg agaaatttgt cgagataagg tacaacataa
      961 ggaacagaag tctggaatat accattttca atccagtaaa gggtgtttgc ccctgggcgt
     1021 aaattaaagg cggtgagata tgcatcagcc gcttcccggt tcatcccctt catttcataa
     1081 accttgccaa gcaacacata atttagccag gacatttcaa gatcaatgcc agtatttatc
     1141 gcctggtaag actcatctgt tttacctttt accagagcac tgaccgcttt tatttgatat
     1201 ataatggaca gattattcaa ttccggcagt gtaacaatgt tgtctatttc tgtgttcagt
     1261 gctgctaatt gtttttcatc taaaggatgt tgagaatggc gcacgatatc aactaatgct
     1321 ttttctgctc tcgcgtaggt aaattctggg gatgattgaa caatctcacc taataattca
     1381 ctggcacggt tcaatgattt atcatcgcca tgcagtaaat aatcatgtgc ctgataaaaa
     1441 ttagttaata acgcaccacg atgaggcaaa atttgctgga gcgtctcctg cattcgttgt
     1501 ggccacggtt ggtttaacgc ttttgataaa ctctccagta aatcattttg aatcgccagc
     1561 tgattaccgt tagtgatgac ataacgttta tccagcatgg ttgaaccatc tgcattgtct
     1621 accaatttta tcgacataaa gcattgttga gcacggtatt ggcgctgatt aacaaacgca
     1681 atagataatg ttttaccgga actgctcggt tcatcaatgt tgtagttgat tttgtcatgc
     1741 accataaagg tggagaaggt gttaagtgat gtcgccacca aattacccac gcctatcgcg
     1801 taagagagct gatacgggga actccagctg ttacaacttt tatttaccat attaatgtca
     1861 atatcgcgtg gattgagcaa aatacgcgat ttgctcatcg gaagacgtgt atcaagagtt
     1921 gaaaacgcta ccagtgctac acagatacct aacgacaaca ggaaaaaaaa ccatacccaa
     1981 aaggtagtga atcgtttgct tttaactggg gattgttcag gtggcgttgc ggtgttttga
     2041 atgttaagac tgtgggaggg agaatctgtg gcaggaaccg cctctggtat agggggaggc
     2101 gaagatagca ttatttcctc tccctcttct tcgctgtacc agataaccgg caccattaat
     2161 ttatagccgc gctttggtac agtagcgata tagacaggac tatcttcatc attatctttt
     2221 aatgacttac gtagttctga gatactctgc gtcacaacat gattggtgac aatacttctc
     2281 ttccagacat tatcgataag ttcatccctg ctaagtactt cgccactgtg ttgagcaaag
     2341 aaaaccagaa gatcgattaa tctcggctca agggtaagtt gacgcccatt gcggctaatt
     2401 tggtttatgg acggagtaac aagccattcg ccaacgcgaa ctacaggttg ttgcataata
     2461 gaaactcatt cgaaaaggga atgatgcaat gataattgcc acaacctatt tttaccatct
     2521 atagatgggt tta
//
LOCUS       DAFATA010000225         2430 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFATA010000225 DAFATA010000000
VERSION     DAFATA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.227"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(97..540)
                     /locus_tag="LGJ20_004800"
     CDS             complement(97..540)
                     /locus_tag="LGJ20_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK2388772.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            697..1626
                     /gene="metA"
                     /locus_tag="LGJ20_004801"
     CDS             697..1626
                     /gene="metA"
                     /locus_tag="LGJ20_004801"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBK2388773.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1895..>2430
                     /locus_tag="LGJ20_004802"
     CDS             1895..>2430
                     /locus_tag="LGJ20_004802"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Catalyzes the aldol condensation of glyoxylate with
                     acetyl-CoA to form malate as part of the second step of
                     the glyoxylate bypass and an alternative to the
                     tricarboxylic acid cycle; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBK2388774.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVT"
BASE COUNT          619 a          628 c          562 g          621 t
ORIGIN      
        1 agtcggaaga ctgggccttt ttgcgctggt gcagaaaaag gtcggatgcg acgctggcgc
       61 gtcttctcca gcctgggcag gcacggcagg ggcggcttat tcccccacat acgccagatt
      121 cagcaacgga tacggtttcc ccaaatcgtc cacctcagag cgtcccgtaa ccttaaaacc
      181 caccttctta tagaacccaa ccgcctgctc attttgctca ttaacgttgg ttgtcagttc
      241 cggtgccatc gagagcgcat gctccaccag catccgaccc acgccgcagc cgcgcacatc
      301 aggatcgata aacagcgcat ccatatgctg cccacttaac aacataaacc caaccggctg
      361 atcccgttca ttaaccgcga cccacaacgg tgcttccggc agaaaggaac gaaccagctc
      421 ttccagctcg gcccgatact ctgctgataa aaaatcgtgg gtggcatcga cagatcgaca
      481 ccaaatcgca acgagttcct ccccttcctc atgctgtgag cggcgaatac taataaccat
      541 tttctctcct tttcgtcatt cttatattct aacgtagcct tttccttgaa actttctcac
      601 cttcaacatg caggctcgac attggcaaat tttctggtta tcttcagcta tctggatgtc
      661 taaacgtata agcgtatgta gtgaggtaat caggttatgc ctattcgtgt gccggacgag
      721 ctacccgccg tcaatttctt gcgtgaagaa aacgtctttg tgatgacaac ttctcgtgcg
      781 tctggtcagg aaattcgtcc acttaaggtt ctgatcctta acctgatgcc gaagaagatt
      841 gaaactgaaa atcagtttct gcgcctgctt tcaaactcac ctttgcaggt cgatattcag
      901 ctgttgcgca tcgattcccg tgaatcgcgc aacacgcccg cagagcatct gaacaacttc
      961 tactgtaact ttgaagatat tcaggaacag aactttgacg gtctgattgt aaccggtgcg
     1021 ccgctaggcc tggtggagtt taatgatgtc gcttactggc cgcagatcaa acaggtgctg
     1081 gagtggtcga aagatcacgt cacctcgacg ctgtttgtct gctgggcggt acaggccgcg
     1141 ctcaatatcc tctacggcat tcctaagcaa actcgcaccg acaaactctc tggcgtttac
     1201 gagcatcata ttctccatcc tcatgcgctt ctgacgcgtg gctttgatga ttcattcctg
     1261 gcaccacatt cgcgctatgc tgactttccg gcagcgttga ttcgtgatta caccgatctg
     1321 gaaattctgg cagagacgga agaaggggat gcatatctgt ttgccagcaa agataagcgc
     1381 attgcctttg tgacgggcca tcccgaatat gatgcgcaaa cgctggcgca ggaatatttc
     1441 cgcgatgtgg aagccggact aggcccggaa gtaccgtata actatttccc gcacaatgat
     1501 ccgcaaaata caccgcgagc gagctggcgt agtcacggta atttgctgtt taccaactgg
     1561 ctcaactatt acgtctatca gatcacgcca tacgatctac ggcacatgaa tccaacgctg
     1621 gattaatctt ctgtgatagt cgatcgttaa gcgattcagc accttacctc aggcaccttc
     1681 gggtgccttt tttatttccg aaacgcacct caggaggtga ataaatttta ttcatattgt
     1741 tatcaataag ttatcaagta tttttaatta aaatggaaat tgtttttgat tttgcatttt
     1801 aaatgagtag tcttagttgt gctgaacgaa aagcgcacaa cgatccttcg ttcacagtgg
     1861 ggaagttttc ggatccatga cgaggagctg cacgatgact gaacaggcaa caacaaccga
     1921 tgaactggct ttcataaggc cgtatggcga gcaggagaag caaattctta ctgccgaagc
     1981 ggtagaattt ctgactgagc tggtgacgca ttttacgcca caacgcaata aacttctggc
     2041 agcgcgcatt cagcagcagc aagatattga taacggaact ttgcctgatt ttatttcgga
     2101 aacagcttcc attcgcgata cggactggaa aattcgtggt attcccgcgg acttacaaga
     2161 tcgtcgagtc gagataactg gtccggttga gcgcaagatg gtgatcaacg cactgaacgc
     2221 caatgtgaaa gtctttatgg ccgatttcga agattcactg gcaccagact ggaacaaagt
     2281 gatcgacggg caaattaacc tgcgcgatgc ggttaacggc accatcagct ataccaatga
     2341 agcaggcaaa atttatcagc tcaagcccaa tccagcggtt ttgatttgtc gggtacgcgg
     2401 tctgcacttg ccggaaaaac atgtcacctg
//
LOCUS       DAFATA010000226         2407 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFATA010000226 DAFATA010000000
VERSION     DAFATA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.228"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..113)
                     /locus_tag="LGJ20_004803"
     CDS             complement(<1..113)
                     /locus_tag="LGJ20_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenoprotein"
                     /protein_id="HBK2388775.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHP"
     gene            complement(295..2400)
                     /gene="cstA"
                     /locus_tag="LGJ20_004804"
     CDS             complement(295..2400)
                     /gene="cstA"
                     /locus_tag="LGJ20_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBK2388776.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
BASE COUNT          608 a          768 c          591 g          440 t
ORIGIN      
        1 tcgggatggt taacccgcat atgttcgaca tagttgtcgt aatcaggcat accaatcatc
       61 agcttcgccg cctgacctaa atattttccg gcttttgcca gtgaatcaaa catcttggcc
      121 tccgctaacc atgttgctga aagagtgccg gatgcggcgt gaacgcttta tccggcctac
      181 aaatgctatc aacgcactcg gtcagtcccc tcgccccatc ggggagaggg ttagggtgag
      241 ggggaaccct cggcactatg ccaacattcg ccaacatccc ccctcactct gactttagtg
      301 cgcacctttc gcctgcgcta cgatctcctc aacattttcc ggcattggct catacggcgt
      361 ttctttcgcc gttggcttcg gctctttcaa tgccgcaagt gccgtcttaa tcgagaacag
      421 tgccagaacc accacgacca ccataaagaa gatggttaac ccggcatcca gacggttgtt
      481 gaacaccagt tgcgccagct gtgactcagt atactgcgac ggaatattgc cgctgtcgat
      541 cattgcctgg aacttgttag caatggccag gaagccgact ttcgcatccg ggctaaacgc
      601 tttctgccag cctgcggtca gggtacaaat cagtagccag gccgttggta ccagcgccac
      661 ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg gcacagagca tcagcgccat
      721 ccctgccagc atctggttgg caataccaaa cagcggccac agagtgttaa taccgcccag
      781 cggatcgacc acgccctgat ggaggaagta gccccacgcc agtacgcaca gcgccgttgc
      841 cagcaggtta gcaggcagtg aatcggtccg cttcaggcca ggagacacca cgcccagcag
      901 atcctgcaac ataaagcgcg cagcacgcgt acccgcatct accgccgtca gaataaacag
      961 agcttcaaac agaatggcga agtgatacca gaacgccaca tccatcatac cacccagcgc
     1021 accgtgcagg atgtaagcca tccccaccgc caacgtcggc gcaccgcccg cacgggaaat
     1081 gatcgactgt tcacccactt cgctggcaat ctggttgagc gtatctggtg taatgctaaa
     1141 gccccagcta ctcaccacct gcgcggcaga agcgaccaca tccgccgtcc ctgccggagc
     1201 cagcaccgcc attgggctgt tcatggcaaa gtaaacgccc ggatcgatga tacaggcgga
     1261 aaccagcgcc ataatcgcca cgaaggattc catcaacatt ccaccgtagc cgataaaaca
     1321 agcctgccct tcgttcgcca gcatcttcgg cgtggtgccg gaagagatca gcgcatggaa
     1381 gccagacacc gcgccacagg cgatggtgat aaacaggaac gggaacaggt taccggtcca
     1441 taccgggcca gtgccatcga caaatttggt cagcgcaggc atggtcagcg tcgggcgcat
     1501 aatcaaaatg cctaccgcca gaccgacgat cgtcccgatt ttcaggaagg tagagaggta
     1561 gtcacgcggg gccagcagca accacaccgg cagcaccgcc gccacaaaac cgtaacccac
     1621 cagcatccag gtcagctgca cgccggtaaa gtcaaagtac ggtgcccagg tcggactttc
     1681 tgccacccag ccgccagaga taatggcgaa aatcaggaat accagaccga tgaccgacac
     1741 ttcaccaata cgccccggac gcagatagcg caggtagatc cccataaaca gcgccagcgg
     1801 aatggtgaac gcaacggtat acgttcccca cgggctatga gtcagggctt tcaccacgat
     1861 catcgccagt actgcaagga taatgaccat gatcataaag caggccacca gcgctatcac
     1921 cccggcggtt ggccccatct cttctttgac cagctcaccc agcgatcgac cgtcacggcg
     1981 cgtagaaaca aacagcacca tgaaatcctg caccgcaccg gcgagaacca ccccggcgag
     2041 cagccagatc atccctggca ggtaacccat ttgcgccgcc agtaccggcc caaccaacgg
     2101 acctgctccg gcaatggccg caaaatggtg accaaacagc actttcttgt ccgtcggcac
     2161 atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata cgcgtcgggt caaccgccag
     2221 cacatttttg gcgatataca gcccataaaa acggtaagcg atcagataga tacagaccga
     2281 cgccaccaca atccacagcg cgttgatctg ttccccacga tttaaagcaa tgtatcccag
     2341 agcaaatgct cccattacag agagcactgt ccagacgagg tatttccctg atttgttcat
     2401 agttgtt
//
LOCUS       DAFATA010000227         2321 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFATA010000227 DAFATA010000000
VERSION     DAFATA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.229"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1496
                     /gene="istA"
                     /locus_tag="LGJ20_004805"
     CDS             <1..1496
                     /gene="istA"
                     /locus_tag="LGJ20_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HBK2388777.1"
                     /translation="LKYGVGLSHRQIGRSLAISPSVVSRYANRAAQLGIKQWPLPTGW
                     DDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLWEEYCERNPGGFYSYNH
                     YCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPETGEIRTAQVIVAVLGAS
                     SYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSATSRACKYDPDVNPTYQ
                     QMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHEIFYSLASLNQRIRELL
                     ERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKKVRVHADYHVEIDKHYY
                     SVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYSTRNEHMPEAHRQHATWT
                     PERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNLHKKYSKARLNAACARA
                     LKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRGSEYYH"
     gene            1511..2257
                     /gene="istB"
                     /locus_tag="LGJ20_004806"
     CDS             1511..2257
                     /gene="istB"
                     /locus_tag="LGJ20_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HBK2388778.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          660 a          556 c          570 g          535 t
ORIGIN      
        1 ggcttaaata cggtgttgga ctcagccatc gacaaattgg tcgtagtctt gcaatctccc
       61 cttccgttgt atccagatat gctaatcggg cggctcaact tggcataaag cagtggccct
      121 tacctacagg atgggatgat acaaaactaa aacatgcgtt ccttcagacc caggttaaga
      181 tgaagaagca ctctctgcct gactgggcta cagtacaccg ggaactgcgt aataaatgcg
      241 tgacgctgca gctactctgg gaagaatact gtgagcgtaa tccaggcggt ttttacagct
      301 ataaccatta ctgccggatg taccgtgaat ggctcaaaac cacttcacca tcaatgcgtc
      361 aggtacataa agctggcgaa aaacttttcg ttgattactg tggacctacc gttggcgtta
      421 ccgaccctga gaccggagaa ataagaactg ctcaggtcat cgtagctgtt ctcggggcat
      481 caagttacac atgggcagag gccacctggt ctcagcagct tgaagactgg gtgatgagtc
      541 atgttcgctg cttccagtgg ttgggtggcg ttcctgaact tgttgttccg gacaatctga
      601 aaagcgccac atccagggca tgtaagtatg atcctgacgt taaccctacc taccagcaga
      661 tgcttgagca ttataatgtc gcagttttgc ctgcgcggcc acgtaaaccg aaagataaag
      721 ccaaagctga agttggcgtt caggttgttg aacgctggat catggcccga atcaggcatg
      781 agatcttcta cagccttgca tcgcttaatc agcgcattcg ggagttgctg gaaagactga
      841 ataacaaaat aatgcagaag ttgggttatt cacgtgcaga actcttcatc cagcttgata
      901 aacccgcact gaagcctctt cctgaagcca gttacagtta caccctggtg aagaaagtca
      961 gagttcatgc cgattaccac gtggaaatcg acaaacatta ctactcggtt ccatgttcgc
     1021 tgttaggcca gcaactggaa gcatggatct ccggagaact ggtaagactc ttcaatcagg
     1081 ggcaggaggt tgctgtgcac ccgcgcaagc gtacttatgg ctacagtacc cgcaacgagc
     1141 acatgcctga agctcatcga cagcatgcca cctggacgcc agagcgtctt ctggaatggg
     1201 cggggcacat aggcagtgaa actcatagtt atgtgcttca tatactgaac tctcgtccac
     1261 atccggaaca aagctatcgc ttctgccttg gactcctgaa ccttcataaa aaatacagta
     1321 aagccagact taatgcagca tgtgcaagag ctctgaaaac aaaggtatgg cgtctgtcag
     1381 gtattaaatc gatcctggaa aaaggtctgg ataaacaacc tgttcaggat ccaaaaccag
     1441 atctgttatc cacgatggaa cacgaaaacg tacgcggcag tgagtattac cactgatacg
     1501 ggatccaatg atgaatcatc tttacgaaca actgaccgca cttaaactca ccggcttccg
     1561 tgatgcgctt aaaaagcaac ttgctcagcc gggcacatac caggagctgg gcttcgaaga
     1621 acgcctgtca ttactgacag cagaagaact aacctgccgt gaaaacagga aggcagagcg
     1681 tctgatcaaa catgcacggt tcagacttaa tgctgagtta tcaaagctgg attatcgtaa
     1741 caatagaggg ctggacaggg ccctcatccg ttcactcagt cagggaaact ggttaaccct
     1801 gaaacaaaat attttactga ccggggccac cggcagcggt aaaacgttcc tggcatgtgc
     1861 acttggtcat aatgcctgcc gacagggata caaggtctac tattatcgcc ttaaagcgct
     1921 gatggaacag tgctatcagg ggcatgctga tggaagatac agcaaacttt tgaccaggct
     1981 gaataatagc gatctgctgc ttctggatga ctggggactg gaacctctct catcagaaca
     2041 gcgtagcgac ctgctggaaa tagtggatct gatgtaccaa cgaggctcaa tcatcgtagt
     2101 gagccagttg ccggtggaaa actggtacaa aatgatcgga gactccacac atgcggatgc
     2161 catcctagat cgactggttc atggcagtat caagatcgaa cttaaaggag aatcaatgcg
     2221 gaaaatacaa tctccgttga ccgaaggaga tcagtgaagg taatttaaaa acggttctgt
     2281 gaaagtgaca cgaaccgatc tccatcgatg ttactcaccg a
//
LOCUS       DAFATA010000228         2280 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFATA010000228 DAFATA010000000
VERSION     DAFATA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.230"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..891)
                     /locus_tag="LGJ20_004807"
     CDS             complement(<1..891)
                     /locus_tag="LGJ20_004807"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="catalyzes the formation of
                     2-hydroxy-3-oxopropanoate (tartronate semialdehyde) from
                     two molecules of glyoxylate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBK2388779.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRK"
     gene            complement(981..1796)
                     /gene="allR"
                     /locus_tag="LGJ20_004808"
     CDS             complement(981..1796)
                     /gene="allR"
                     /locus_tag="LGJ20_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBK2388780.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(1874..>2280)
                     /gene="allA"
                     /locus_tag="LGJ20_004809"
     CDS             complement(1874..>2280)
                     /gene="allA"
                     /locus_tag="LGJ20_004809"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBK2388781.1"
                     /translation="FFHINNGLVERYHDLALVEILEQDRTLISINRAQPANLPLTIHE
                     LERHPLGTQAFIPMKGEVFVVVVALGDDKPDLSTLRAFITNGEQGVNYHRNVWHHPLF
                     AWQRVTDFLTIDRGGSDNCDVESIPEQELCFA"
BASE COUNT          562 a          648 c          548 g          522 t
ORIGIN      
        1 tttgcgccct tcggtgtatt tctctaccga gccggtatga cggttagcaa aacggttacc
       61 gataccaaac accatgtcag acgccagcag cgttgcgtta ccgtaacgat gcgcggtttg
      121 cagacccacc atcccggcca tcagttcatg atcgtccggg atacagcccc agcccattag
      181 cgttgggatc accggaacgc tggtcagttc agcaaactgt tgtaacagtg cagctgcgtc
      241 agcattaatt accccgcccc cggcaacaat caccggacgt tcggcctgga ttaacatttc
      301 tacagctttt tcgatctgca tacggctggc agcaggtttg tagaccggca gcggttcgta
      361 catgtcagga tcaaactcga tttccgcaac ctgaacgtcg aacggtaaat ccaccagtac
      421 cggacccgga cgaccagaac gcatcaggtg aaatgcctgt tgcagcacgc gaggcaccag
      481 cgccgcttca cgaactgtaa ccgccatttt gctgaccggt ttagcaattg cttcaatatc
      541 tacggcctga aaatcttctt tatgcagacg ggcgcgcggt gcctggccgg taatgcacag
      601 aataggaatg gaatcagcag aagcggaata gagcgcggtg atcatgtccg tgcccgcagg
      661 accggaagtc cccagacata cgccgatatt tcctgccgtt gcgcgggtat aaccttccgc
      721 catgtgcgaa gcaccttcca catgacgcgc cagaatgtga cgaataccgc cgtgcttacg
      781 catcgctgag tagaacggat tgattgcagc tcccggaaca ccgaaggcgg tggtgatacc
      841 ttctttctcc agcacataca ttgccgcgtc aacggctctc atttttgcca ttttattcct
      901 acctctattt attggaaaat ttttccaact ttcaaatttt gtcaaaaaaa cccgctccgc
      961 agagcgggat gcgacagaca ttatggatgt gctttcagtc ccaacgccgt gctgatatcg
     1021 cgggcggtgt ctctgaccag ctcaccctga ctgacaaaac gatcttctgt cagtcttgat
     1081 gaaggcccgg agatggagat agcggcaaca acactaccga catcatcgta aattgctgaa
     1141 gctatgcaat tcagacctac aacatgctct tctttatcta cggtatagcc cagttcacgc
     1201 gcttgttcca ggtccttcag caaggtgggc atatccacaa gcgtagttgg cgtaaactgc
     1261 tgcaaaccgg tttgcagaat gatgctcatc aactcctctt ccgccagcgg ataaagcagc
     1321 gctttgcccg caccggaagc atgcagcggc agacgactgc ccagtggcgc acacatcctg
     1381 accatcgatt tacactctaa ctgaccaatt aatactgctt cattgccgtt acggatcgcg
     1441 acattgaccg tttcgccgga aagtaacatc aggcggcgca taaacggccc ggcgacggag
     1501 aggacatcgc ggttatggat gtacgctgca ccgacgttaa agacacctaa tcctatatgc
     1561 caccagccta attgactgtc ctgatagaca aaatccgctg cttgtaaaac cttcagcaag
     1621 cgaaaggtcg tggagagcgg caaatccaga ttgagagaaa tatcgctaac cgacgaactt
     1681 cccccacttt tttccagata ttgcagaatc gcaattcccc gctctaacgc ctgtgcgccc
     1741 ttctgtgcca caggctccgc ctgtcctggc ctgccgcgcc gtctaacttc cgtcatacct
     1801 acgttaactc cgacctacaa gtgagcataa acaatgaaca attagtttat cttatgcaag
     1861 tcggttgcag gcgtcacgca aaacagagtt cctgttcagg aatactttca acatcacagt
     1921 tgtcactgcc gccgcgatcg atggtcagaa aatcggtgac gcgctgccag gcgaaaagtg
     1981 ggtgatgcca gacgttacga tggtaattca ctccctgttc gccgttggtg ataaacgccc
     2041 gcagcgttga caggtctggt ttgtcgtcgc ctaacgccac gaccaccaca aacacttcac
     2101 ctttcatcgg gataaaggcc tgagtgccca gcggatgacg ttcgagttcg tgaatggtca
     2161 gcggcagatt cgccggttgc gcgcggttaa tgctgataag cgtacggtct tgctcaagaa
     2221 tctcaaccag cgccaaatcg tggtaacgct ccaccaggcc attgttaata tggaaaaaat
//
LOCUS       DAFATA010000229         2231 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFATA010000229 DAFATA010000000
VERSION     DAFATA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.231"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..830
                     /locus_tag="LGJ20_004810"
     CDS             <1..830
                     /locus_tag="LGJ20_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBK2388782.1"
                     /translation="LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAI
                     LSALNTPDRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
                     RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLV
                     RRLCPQCRQFTPVSPQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVT
                     PELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            827..2023
                     /gene="gspF"
                     /locus_tag="LGJ20_004811"
     CDS             827..2023
                     /gene="gspF"
                     /locus_tag="LGJ20_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBK2388783.1"
                     /translation="MNYLYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            2033..>2231
                     /locus_tag="LGJ20_004812"
     CDS             2033..>2231
                     /locus_tag="LGJ20_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK2388784.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHHY"
BASE COUNT          560 a          601 c          560 g          510 t
ORIGIN      
        1 agctttcatt gaacaacctg gggatgacgg cagcggataa gcaggattta gaaaatctca
       61 ttcagcttcc gcacggtatt atcctggtga cagggccgac aggctccggt aaaagcacca
      121 cgctctacgc catcctttcg gcgctgaata ctcccgaccg caatattctg acggtagaag
      181 atcccgtgga atatgagctg gaaggcattg ggcaaacgca ggtgaatacc cgtgtggata
      241 tgtctttcgc tcgcggcctg cgcgccatac ttcgccagga cccggatgtc gtcatggtgg
      301 gggaaattcg tgatacagaa accgcgcaga ttgcggttca ggcctcgctc accggccatc
      361 tggtactctc aacactccac actaacagtg catcaggcgc agtgacccgg ctccgcgaca
      421 tgggcgtcga atcattcctg ctttcgtctt ccctggcagg gattatcgcg caacgtctgg
      481 ttcgtcgcct gtgtccgcaa tgccgacaat tcacgcccgt atcaccccaa caagcgcaga
      541 tgtttaaata tcatcagctc gcggtgacaa caattggcac tcccgtaggc tgccctcatt
      601 gccatcaatc cggctatcag gggcgcatgg cgatccacga aatgatggtg gtgacgccgg
      661 aattacgggc cgctattcat gaaaatgtgg atgaacaagc actggagcga ctagtccggc
      721 aacaacacaa ggccttaatc aaaaatggcc tgcaaaaagt gataagcggt gacacctcct
      781 gggatgaggt tatgcgcgtc gccagtgcca cgctggagag cgaagcatga attatctcta
      841 tcgcgccatg acccaggatg gtcaaaaatt gcaagggatc attgatgcta acgatgaacg
      901 tcaggcacga ctgcggctgc gtgaagaagg gcttttcctg ctggatattc gcccccaaaa
      961 aagttcggga gtaaaaacac gtcgcccgag gatcagccat agtgaactga cgcttttcac
     1021 ccggcagttg gcaaccttaa gcgcagcggc attacccctg gaagagagcc ttgccgtaat
     1081 cggtcaacaa agcagtaata aacgactggg tgacgtgtta aatcaggtac gcagcgccat
     1141 ccttgaaggg catccccttt ccgatgcatt acagcatttt cccacgcttt tcgattcgct
     1201 ctatcgtacc ctggtaaaag cgggcgaaaa gagcgggctg ctggccccgg tgttggaaaa
     1261 gctggctgat tacaatgaaa accggcagaa aatccgcagc aagctcattc agtcactgat
     1321 ctacccctgt atgctcacta cggtggcgat tggggtcgtg attattctcc tcactgctgt
     1381 cgtgcccaaa attaccgaac agttcgtgca tatgaagcag caactgccgc tgagtacacg
     1441 cattctttta ggtctgagcg acacgttgca acgtaccggc ccgacattat tagcgacagt
     1501 gtttattgtc gctgtaggtt tctggctctg gttaaaacgc ggcaataacc gccaccgttt
     1561 tcatgccatg ttgctgcgcg ttgcgctcat cggcccgctg atttgcgcca ttaacagcgc
     1621 acgctatctc cgcactttaa gtattttgca atccagcggc gtccctctgc tggatgggat
     1681 gaatttgtcc accgaaagcc tcaacaacct cgaaattcgc cagcgtctgg caaatgcggc
     1741 agagaacgtt cgccagggta acagcattca tctttcgctg gaacaaaccg caattttccc
     1801 gccgatgatg ctctacatgg tggcctctgg cgaaaaaagc gggcagctcg gcacattaat
     1861 ggtcagagcc gcagataacc aggagacact ccaacaaaat cggatcgcct taacgctctc
     1921 catcttcgag ccagcactca ttattacgat ggcactgatc gtcctgttta ttgtcgtgtc
     1981 ggtactccaa cctcttcttc aacttaactc aatgattaat taaggaaagc atatgcgcgc
     2041 aacggataag caacgcggtt ttacattact ggaaattatg gtggtgatcg tcattatcgg
     2101 cgtacttgcc agcctggtgg ttcctaacct aatgggcaat aaagaaaagg cggataagca
     2161 aaaagccgtc agcgatattg tggcgctgga aaacgccctt gatatgtaca aactcgacaa
     2221 tcatcactat c
//
LOCUS       DAFATA010000230         2208 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFATA010000230 DAFATA010000000
VERSION     DAFATA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.232"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..295)
                     /locus_tag="LGJ20_004813"
     CDS             complement(<1..295)
                     /locus_tag="LGJ20_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388785.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNAT"
     gene            complement(341..682)
                     /locus_tag="LGJ20_004814"
     CDS             complement(341..682)
                     /locus_tag="LGJ20_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388786.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(851..>2208)
                     /gene="xylB"
                     /locus_tag="LGJ20_004815"
     CDS             complement(851..>2208)
                     /gene="xylB"
                     /locus_tag="LGJ20_004815"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBK2388787.1"
                     /translation="SRPHPLWSEQDPEQWWQATDRAMKALGDQHSLQDVKALGIAGQM
                     HGATLLDAQQRVLRPAILWNDGRCAQECTLLEARVPQSRVITGNLMMPGFTAPKLLWV
                     QRHEPEIFRQIDKVLLPKDYLRLRMTGEFASDMSDAAGTMWLDVAKRDWSDVMLQACD
                     LSRDQMPALYEGSEITGALLPEVAKAWGMATVPVVAGGGDNAAGAVGVGMVDANQAML
                     SLGTSGVYFAVSEGFLSKPESAVHSFCHALPQRWHLMSVMLSAASCLDWVAKLTGLCN
                     VPALIAAAQQADESAEPVWFLPYLSGERTPHNNPQAKGVFFGLTHQHGPNELARAVLE
                     GVGYALADGMDVVHACGIKPQSVTLIGGGARSEYWRQMLADISGQQLDYRTGGDVGPA
                     LGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLM
                     A"
BASE COUNT          623 a          601 c          476 g          508 t
ORIGIN      
        1 atgtcgcatt ccacagacct accatcagta atgcaacaga gataataaag acagtcaggc
       61 aggtcatata ataaatggaa gtggtcggta tgccttcata cttgtcccga acggtctttt
      121 gataagacgc agcagaaaac agtcctaaca ccagtacggc aaaataatac cctttttcat
      181 ttaactgcat ctctgcattc catagcccta acagataggt aacgatacca ccaacgagag
      241 ctatccatga cacaatacta aaggccggtg aataggttga tattttgttg tccatcacag
      301 tattcctttc attcctgaat gtgtaagagc tttatgttgc tcataccgag cgaaacaaac
      361 gcatttgacc aaacaagcag acaaaaaagg cgatggggta gatcaccatt tcataagcat
      421 tgataggtgc atttaaaaca ccgataaaca agagaccgat agtgattaac gcaactaact
      481 ggcagatatg ggtaaaaaaa ttatccagtt gcccgagttt ttctgcgcga agataagcat
      541 agttaccaaa agttgctgtc attaacacgc caagaaaata gccttttcca cttagcaatg
      601 ggcatgagat ccatagccca accagataaa ccagcgcccc gacaacaaat aatagttttg
      661 ccacagtttt tgaggttttc ataacgatct ccatatctac cagcgataca ttacgagtaa
      721 ccaacgaaag acaaaactga aaaatgccat taacaaatga ttttcagaat aaattcatac
      781 taaatattaa ttaattagtg agatatatag atgtgaatta tcccccaccc ggtcaggcag
      841 gggataacgt ttacgccatt aatggcagaa gttgctgata gaggcgacgg aacgtttcac
      901 gtcgtggctg ataagcggca taacgctgcg catctggtag atgcgactgt tctaacggta
      961 gctgcggcaa caactcaatg agcgatttct ctggattcgc cgcaatctgc gccagccttg
     1021 ctgcgcccag tgctggcccc acatcccccc ccgtacggta atcgagctgc tgaccgctga
     1081 tatccgccag catctgacgc cagtactcac tacgcgcccc gccaccaatc aacgtaacac
     1141 tttgcggttt aatgccgcag gcatgcacga catccatgcc atctgccagc gcataaccca
     1201 cgccttccag cactgctcgc gccagttcat tggggccatg ttgatgagtc aaaccaaaga
     1261 aaaccccctt cgcctgggga ttattgtgtg gtgtacgctc gccggaaaga taaggcagaa
     1321 accaaactgg ctcggcactt tcatcagcct gttgagctgc agcgattaaa gctgggacat
     1381 tgcacaggcc ggttaatttc gcgacccaat ccagacacga tgctgcactc agcattacag
     1441 acattaaatg ccaacgctgc ggtagcgcat ggcaaaagct gtgtacagcg ctttctggct
     1501 tgcttaagaa cccttcgctg acagcaaaat agacccccga cgtccccagc gataacattg
     1561 cctgattagc atcaaccatt cccacaccaa ctgcaccagc tgcattgtcg ccaccgcctg
     1621 caacaactgg caccgtcgcc ataccccacg ctttcgcaac ttcaggtaac aaagcaccag
     1681 taatttcgct gccttcgtat aatgcgggca tctggtcacg agataagtcg caagcctgca
     1741 gcatgacgtc actccagtca cgctttgcga catccagcca catggtgcca gctgcgtcag
     1801 acatatcgct ggcaaactcc cccgtcatac gcagacgcaa gtaatctttc ggtaataata
     1861 ctttgtcgat ttgacggaat atctccggct catgccgctg aacccatagc aatttaggcg
     1921 cagtaaatcc gggcatcatc aggttgccgg taatcactcg tgattgcgga actctcgctt
     1981 ccagcaaagt gcactcttgc gcacagcgcc cgtcgttcca caaaatggca gggcgcaata
     2041 cccgttgttg agcatccagt aaggttgctc catgcatctg gccggcaata cccaatgctt
     2101 taacgtcctg cagagaatgc tgatcgccca gagctttcat tgcgcgatca gttgcctgcc
     2161 accactgttc cgggtcttgt tccgaccaga gtggatgcgg gcgcgaaa
//
LOCUS       DAFATA010000231         2146 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFATA010000231 DAFATA010000000
VERSION     DAFATA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.233"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(51..347)
                     /locus_tag="LGJ20_004816"
     CDS             complement(51..347)
                     /locus_tag="LGJ20_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBK2388788.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(561..1847)
                     /gene="thrC"
                     /locus_tag="LGJ20_004817"
     CDS             complement(561..1847)
                     /gene="thrC"
                     /locus_tag="LGJ20_004817"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBK2388789.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVESILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1848..>2146)
                     /locus_tag="LGJ20_004818"
     CDS             complement(1848..>2146)
                     /locus_tag="LGJ20_004818"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="catalyzes the formation of O-phospho-L-homoserine
                     from L-homoserine in threonine biosynthesis from asparate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBK2388790.1"
                     /translation="ACYSRQPELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVA
                     SGISGSGPTLFALCDKPDTAQRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
BASE COUNT          486 a          596 c          539 g          525 t
ORIGIN      
        1 cagcgtcaca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
       61 tgtttgcctg gaccatgacc gccgtgatga tcatgatgag ctttcttatg gtggcgcggc
      121 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
      181 cagccgtggt cgcgccagtg accgccatcc cagtaataac cgcgattatc acgatcgcct
      241 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgcgctgc catgggagcg
      301 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
      361 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacgg cggttgttat
      421 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
      481 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
      541 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
      601 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
      661 agatccaacg tttcaccgag aatcgattcc acgctctctt taaatttcgc cggatgcgcg
      721 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
      781 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
      841 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
      901 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
      961 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
     1021 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt cgccagcaga
     1081 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgct
     1141 tcctgtggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
     1201 atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc atcatcaaac
     1261 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
     1321 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
     1381 aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc cgctccggta
     1441 tcaccggagg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt
     1501 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
     1561 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
     1621 acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc
     1681 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
     1741 ggcaggtcat gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
     1801 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
     1861 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
     1921 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
     1981 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
     2041 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
     2101 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgt
//
LOCUS       DAFATA010000232         2116 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFATA010000232 DAFATA010000000
VERSION     DAFATA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.234"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            9..845
                     /locus_tag="LGJ20_004819"
     CDS             9..845
                     /locus_tag="LGJ20_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HBK2388791.1"
                     /translation="MKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAY
                     LSAYGCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKID
                     EQTGWHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSR
                     CLSDDPLFAFENYTRTAQVLTDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRRLVSQ
                     GYLIGGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQV
                     SA"
     gene            855..1370
                     /locus_tag="LGJ20_004820"
     CDS             855..1370
                     /locus_tag="LGJ20_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBK2388792.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            1425..1727
                     /locus_tag="LGJ20_004821"
     CDS             1425..1727
                     /locus_tag="LGJ20_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBK2388793.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            1742..1861
                     /locus_tag="LGJ20_004822"
     CDS             1742..1861
                     /locus_tag="LGJ20_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBK2388794.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            1854..>2116
                     /locus_tag="LGJ20_004823"
     CDS             1854..>2116
                     /locus_tag="LGJ20_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388795.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMG"
BASE COUNT          525 a          550 c          618 g          423 t
ORIGIN      
        1 aaacaggtat gaaagccctg ttatctgccc agtcacagct cggcgttaaa ccgcgcattc
       61 tcggcgtgcc aggccacgac accaaggcgg tagctactga gttgctgagc gtggcgcaaa
      121 gcctgcgtgg atttgcttac ctgtcagcgt atggctgcaa gacagtgcag gaggcgatca
      181 cttaccgcga aaacttcagc cagcgcgaag ggatgctgat ctggcctgac tttactggct
      241 gggacacggt gctgaatgcc gaagcaacgg catatgccac cgcccgtgcg cttggtctgc
      301 gcgccaaaat tgacgagcag accggatggc acaaaagcct gtccaatgtg ggcgtgaacg
      361 gtgtcaccgg aatttctgca gatgtgttct gggatctgca ggacccggca accgatgcag
      421 gtctgctgaa ccagaacgac gtcaccacgc ttgtgcgtaa agacggtttc cgcttctggg
      481 ggtcccgctg cctgagtgat gacccgctct ttgccttcga aaactacacc cgcacggcgc
      541 aggtgctgac ggacacgatg gcagaagcac acatgtgggc ggtggataaa ccgcttaacc
      601 cgtcgctggc ccgcgacatt atcgaaggta tccgcgccaa aatgcgcaga ctggtcagtc
      661 agggctatct cattggtggt gattgctggc tggatgagtc ggtgaacgac aaagacacgc
      721 tgaaagccgg aaaactcacc atcgactacg actacacgcc agtgccgcca cttgaaaacc
      781 tgatgctgcg tcagcgcatc accgatcagt acctggtgaa tttcgccagc caggtcagcg
      841 cgtaagggga caacatggct ttaccacgca aattaaaaca cctgaacctg tttaacgacg
      901 ggaacaactg gcaggggatc gttgagtcgc tgacgctgcc gaaatttacc cgcaaatatg
      961 agaagtatcg cggcggagga atgccgggtg cggtggatgt ggatctgggg cttgatgaca
     1021 gtgcgctgga cacagaattt tccattggtg gtactgaatt gctgctgttt aaacagatgg
     1081 gtaaagccac ggtggatggc atccagctgc gctttaccgg ctctattcag cgtgacgata
     1141 ccggggaagt gcaggccgtg gagcttgtcg tgcgtggacg tcacaaagaa gtggattccg
     1201 gcgagtggaa gacgggcgaa agcaacacca ccaaagtgac cagtaccaac agctacgcga
     1261 agctgaccat caatggtgag gtgctctatg aagtggacct tatcaacatg gtggaaattg
     1321 tggacggtgt ggacctgatg gaagcgcacc gcaacgccct cggcctctga tatatctgaa
     1381 cggcgcggga taccgcgcca gaacccaatt gacaggacag caaaatgagc gataagcaga
     1441 ctgaaaagac cattcaactg gataccccca tcaagcgcgg taaaacagaa atcaccgaaa
     1501 ttgtgctgcg taaaccgcag tccggtgcgc tgcgcggtac acgcctgcag gccattatgg
     1561 atatggatgt aaacgcgatg atgaccgtga tcccccgcat ctccagtccg gcactgactg
     1621 cacaggaaat tgcagagatg gacccggcag atctcactgc catgtcggtt gaggttgtca
     1681 cttttttgtt gaagaagtcg gtgcttgccg gtttaccgac agcctgacgg ttgacgatct
     1741 ggtggcagat atcgccacca tttttcactg gccgccatcc gttactgacg ttatgccgct
     1801 gaccgaagtg ctggaatggc ggtataaagc gattcagaga agcggggcca acgatgagtg
     1861 ataacaacct gcgtctgcag gtcattctta atgcggttga caagctcacc cgcccatttc
     1921 gatctgcgca ggccagttca agagaactgg ctgctgctgt caaaaaatcc cgcgatgcaa
     1981 taaagcagct tgatcaggcc gggagcagtc tggacagctt ccgaaagctg caggcagaaa
     2041 atcagaaatt aggcgacagg ctgaactatg cccgccagcg tgcaaatttg ctcagtcagg
     2101 aactgggagc gatggg
//
LOCUS       DAFATA010000233         2088 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFATA010000233 DAFATA010000000
VERSION     DAFATA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.235"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..1098
                     /gene="cfa"
                     /locus_tag="LGJ20_004824"
     CDS             <1..1098
                     /gene="cfa"
                     /locus_tag="LGJ20_004824"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBK2388796.1"
                     /translation="RIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGES
                     YMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASARLFNLQSKKRAWIVGKEH
                     YDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCG
                     WGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSV
                     GMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPS
                     VRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPEIADNYSERFKRMFTYYL
                     NACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(1138..1779)
                     /gene="ribE"
                     /locus_tag="LGJ20_004825"
     CDS             complement(1138..1779)
                     /gene="ribE"
                     /locus_tag="LGJ20_004825"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBK2388797.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
BASE COUNT          512 a          507 c          484 g          585 t
ORIGIN      
        1 cgtatcgcca acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg
       61 gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc tgcaagaagg ctctttgggg
      121 ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc gactggatat gttttttagc
      181 aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga cacgctgcgt
      241 attgccagcg ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaag
      301 gagcattacg atttgggtaa tgacttgttc agccgcatgc ttgatccctt catgcaatat
      361 tcctgcgctt actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa
      421 atgatttgtg aaaaattgca gttaaaacca gggatgcgcg tactggatat tggctgcggc
      481 tggggcggac tggcgcacta catggcatct aactatgacg taagcgtggt cggcgtcacc
      541 atttctgccg aacagcaaaa aatggctcag gaacgctgtg aaggcctgga tgtcaccatt
      601 ttgctgcaag attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg
      661 ttcgagcacg tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg
      721 aaaccggaag gtatattcct gctccatact atcggttcga aaaaaaccga tctgaatgtt
      781 gatccctgga ttaataaata tatttttccg aacggttgcc tgccctctgt acgccagatt
      841 gctcaatcca gcgaacccca cttcgtgatg gaagactggc ataacttcgg tgctgattac
      901 gatactacgt tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat
      961 aactacagtg aacgctttaa acgaatgttt acctattatc tgaatgcctg tgcaggtgct
     1021 ttccgcgccc gtgatattca gctctggcag gtcgtgttct cacgcggtgt tgaaaacggc
     1081 cttcgagtcg ctcgctaaag gctattctat cgccccctct ccgggggcga tttcagatca
     1141 ggcttctgta cctggttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt
     1201 atctaccact gcctgagttt gtggatcaat ttcaatgttg acgcgtgcgc cgagtttttt
     1261 cttcccaaga gtcgtgcgtt ccagagtttc cggaattaaa tggacgcaaa aacgcgttgg
     1321 cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat
     1381 atatttcatc aactgactat cctggacttt aaaccagatc tggcgattat tttccgaggt
     1441 taatattttc gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat
     1501 ttcatcactg aatttcgccg cacgctcaac gtttacccaa tcccccactt ttaaatcgcc
     1561 aagattggta atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat
     1621 ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa gcacctgttt ccaggccgtc
     1681 cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa tttggtttct cgtcaatcga
     1741 caccagtttt gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag
     1801 ttaagacatt ctgttcagca caatagcagg tggcaaacgc ccttaccagt gaaggggtaa
     1861 gaatgactat tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc
     1921 ctgcttcttc tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat
     1981 aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat
     2041 cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcga
//
LOCUS       DAFATA010000234         2041 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFATA010000234 DAFATA010000000
VERSION     DAFATA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.236"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..852
                     /locus_tag="LGJ20_004826"
     CDS             <1..852
                     /locus_tag="LGJ20_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388798.1"
                     /translation="SGISQVFNWFKSLLSPMESSKETLDKCTSAGEIFGNVLGGALQL
                     VLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQDKVALFQGDIAKINP
                     PKSAENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVDETKKRIGPGDIVFK
                     NLPRALAVRGEWQERKIAQVSKPAPAINITSVVPAPLPPALVPVVAASSRPVAEAIRS
                     PVASVPATSRNREPAVSGFGGEIHVHLHNVVTQNPRELAKLVGEMVRAEMERRARAGR
                     GSFYDKD"
     gene            865..1353
                     /locus_tag="LGJ20_004827"
     CDS             865..1353
                     /locus_tag="LGJ20_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388799.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1380..1979)
                     /locus_tag="LGJ20_004828"
     CDS             complement(1380..1979)
                     /locus_tag="LGJ20_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2388800.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYDIGISTIYRYHPVGDIQAEETTRQTQENENR"
BASE COUNT          486 a          517 c          534 g          504 t
ORIGIN      
        1 agcgggatca gccaggtgtt taactggttt aaatcgctgc tgtcaccgat ggagtccagc
       61 aaggaaacgc tggataaatg taccagtgct ggcgagatat tcggtaacgt tcttggtggc
      121 gcgctacagc ttgttctgac gcctgcaaaa atgttgctgg atacgctggc gtggatactt
      181 gaaaaactcg gtgtgcttcc ggatgaagcg gaaagggcga gaaagaaaat cgaagacgca
      241 cagcgtgcgg ccattcttca ggacaaggtt gctctgtttc agggagacat tgcgaaaatc
      301 aatccgccga agtctgcgga aaatggcaat ggcaccggag gcgataaacc caaagacaac
      361 aaaccgctca cagacagcaa taccggcacg ctacgcagac tcagcaaaat tgctgataac
      421 acaggtaagc tggttgatga gacaaaaaaa cgtattggcc ccggcgatat tgtctttaag
      481 aacctgcccc gcgcacttgc tgttcgtggg gagtggcagg agcggaagat tgcgcaggtc
      541 agtaagcctg cccccgcaat taatatcaca tccgtagtcc cggctccgct gcctccggcg
      601 ctggtccctg ttgttgcggc cagctcccgc ccggtggcgg aggccatacg atcgccagtg
      661 gcatcagttc ctgcaacttc ccgtaaccgg gagcctgctg tctccggatt tggtggtgaa
      721 attcatgttc atctgcataa cgttgttacg cagaatcccc gcgaactggc gaaactggtc
      781 ggtgaaatgg tcagggcaga aatggaacgg cgcgcccgtg ccgggcgtgg cagtttttac
      841 gataaagatt gaggagtcat ggccatgatg atgatctacg gcatgtttgt ttttgagctg
      901 cgcacgctgc cgcatcagca gttacagcaa aacaaaagct ggcggcatgt gaaaaatgag
      961 cgcgtaaacc gctcagcaag ctggcagtat atcggtgcag gtgatgatcg catcgtgctt
     1021 tctggcgtgc tttatcctga aattacaggt ggcgaagtgt cgctttcgct gctgaccacg
     1081 caggcatata caggacgccc ctggcctctg attgatggtg tcgggcagat ttacggcatg
     1141 tatgtactga ctgaaacgaa tacgacccgc tccgagtttg atcgctacgg taaggcgaaa
     1201 aagatagaat tttcactgac tcttgaacgc tgtgatgagg atttgcggga gcgcctgcaa
     1261 tcctcatcgt tcagtgatat gctgtccggc ttcaaagata aggtcacatc atcccttaac
     1321 agcgcggcca gctccgttaa agggctgttt tgattaacgc aaaaccgcta atggtcagat
     1381 tagcggtttt cattttcctg agtctgcctg gttgtttctt cagcctgtat atcgcctaca
     1441 gggtgataac gataaatcgt cgatatgccg atgtcgtaaa tgatcgccag ttgtttcctg
     1501 tcatgaccgt ttttgatcag ccttgctatt tgctcatgct gttcttttgt cagcttcggg
     1561 cgacgtccgc ctgtgcgccc ccgtgcgcgc gctgccgcca gtccggccag tgtacgttca
     1621 acaattaatt cacgttccat ttcagccagg gcacccataa cgtggaagaa aaaacgcccc
     1681 attggagaag atgtatctat gctgtcggtc agactgcgaa aattaatccc tcgctcccgt
     1741 agttccccga cgagagaaat cagatgtttc atgcttcgcc cgaggcgatc cagtttccag
     1801 acaaccagcg tgtcaccttt ttgaagccgc tttaaagcgc gttttaatcc cggtcggtct
     1861 gtctttgtcc cgcttaattt atcttcaaat atttgttcac atcctgcgca aacaagagcg
     1921 tttcgttgca ggtctgtatt ctggtcattt gttgataccc ttacataacc aatcagcaca
     1981 ctgaatctcc cgtccaaaag cgcaaatcat gccatgcagg ccggaaacgg ccattatcta
     2041 a
//
LOCUS       DAFATA010000235         1984 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFATA010000235 DAFATA010000000
VERSION     DAFATA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.237"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..699)
                     /locus_tag="LGJ20_004829"
     CDS             complement(<1..699)
                     /locus_tag="LGJ20_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK2388801.1"
                     /translation="MAYLDPYQRPAPDRFVRRGLFITACIAALMLLWQFLPAIEAWFS
                     PREAAERTVMARGDLAADEKTTIELFEKSRASVVYITTAQLVRDVWTRNVFSVPRGTG
                     SGFIWDDAGHVVTNFHVIQGASEATVKLADGRDYQAALVGTSPAHDIAVLKIGVGFKR
                     PPAVPVGTSADLKVGQKVFAIGNPFGLDWTLTTGIVSALDRSLPGEAGGPAIDHLIQT
                     DAAINPGNSGGPLLD"
     gene            complement(724..1690)
                     /locus_tag="LGJ20_004830"
                     /pseudo
     CDS             complement(724..1690)
                     /locus_tag="LGJ20_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
     gene            complement(1668..>1984)
                     /gene="psiE-GI"
                     /locus_tag="LGJ20_004831"
     CDS             complement(1668..>1984)
                     /gene="psiE-GI"
                     /locus_tag="LGJ20_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat resistance protein PsiE-GI"
                     /protein_id="HBK2388802.1"
                     /translation="NPLDHKVFQSVFGMIMTLLIAMEFKHSIVRVALRRDSIIQVKTV
                     VLIALIALSRKFVILDSDASPAKIAALASATLALGATYWLLRKRGDRAAETSEHDPSS
                     SQ"
BASE COUNT          361 a          629 c          690 g          304 t
ORIGIN      
        1 atcgagcagc gggccaccgg aattgccggg gttgatggcg gcgtcggtct ggatcaggtg
       61 atcgatggcc gggccgcccg cttctcccgg caacgagcgg tcgagcgcgg agacgatacc
      121 agtggtgagc gtccagtcca ggccgaaggg gttgccgata gcgaacacct tctgacccac
      181 cttgaggtcg gcactggtgc cgaccggcac ggccggcggg cgcttgaaac cgacgccgat
      241 cttgagcacg gcgatgtcgt gcgcggggct cgtccccacc agcgcggcct ggtagtcgcg
      301 gccgtcggcc agtttgacgg tggcttcgga cgcgccctgg atgacgtgga agttggtaac
      361 gacgtggccg gcgtcgtccc agatgaagcc cgagccagtg ccgcgcggca cggagaacac
      421 gttgcgcgtc cagacatcgc gtactaattg cgcggtggtg atgtagacca ccgaggcgcg
      481 cgatttttcg aacagttcga tggtggtttt ctcgtccgcc gccagatcgc cacgcgccat
      541 cacggtgcgt tctgctgcct cgcgcggact gaaccatgcc tcgatggcgg gcagaaactg
      601 ccacagcagc atgagcgcgg caatgcaggc ggtgatgaag agcccgcgcc ggacgaagcg
      661 gtccggcgca gggcgttggt acgggtcgag ataggccatg agacgtctcc tgttgatggg
      721 caatcagcgc cacagcccgc tcgggcgcca gcgcggtggg cgcgtcgcca aagcggattc
      781 cggcaaaaaa tggggcgcgc ggagtggcaa cgctgatgct ggcccaggcg ccaacgtcag
      841 caggcgtgcg atgcgctctt gcgttgccgg acgcgtgcac aagcaggagg gctcgggatt
      901 gccccatccc ggccatagcc aggcacgcca ggagcggctg acccgcgcga ttttcgccag
      961 cgcggacgcc agcccctgcg ggtcgccagt cagttccgcc gcaaggcggt cagcgtcgaa
     1021 ctcacgcacg cgagacaagc cgagctgtgc gagcagggcc agctggggcg atgcggccaa
     1081 taacaataga ccaggccaat agacctccgc cgcgccaacc agctgcgcgg gcaggctgag
     1141 caggatcgcg atctgtccca taagggccag taggctcgtg aggcgactgt tggaatccgc
     1201 cagccccatg acacgcagat cctcattagc gatgtgcgcg acctcgtgca cgagcacacc
     1261 cgccagctcg cgtgggctca ggctgcgcag caggccatcg gtgagggcga tcgatgcctc
     1321 ctgcttcgat cccggtggcg aaggcgttga cgacggcact gggcacatag tgcggcaccg
     1381 gcgtggcggg caaaccggca cgggcgggca gctcgcgcaa cagggcccac atctcgtggg
     1441 cttcttgcgg gtgcaaggcc cgtacgcggt acaggcgcaa gctcagcgcc gacgcggcta
     1501 ccggttccag cagcaacgca cctgcaacgg caaacagcgc gagccacagg cagccttccc
     1561 caaacggcag tctccctgct gcggctgcga tccctaccat ggtcaggacc aacagcccgg
     1621 tctgcaggcg gttgagccag cggtgttgca agagcgccgt gcgcggatca ctgggatgat
     1681 gacgggtcat gctcagaggt ctcggcggcg cggtcgccgc gcttgcgcag cagccagtag
     1741 gtcgcaccca gagccagcgt ggcgcttgcc agcgcggcta tctttgcggg gctcgcgtcg
     1801 gagtcgagga tcacgaactt gcgggagagt gcgatcagcg cgatcaggac caccgtcttg
     1861 acctggatga tgctgtcccg acgcagcgcc acgcgcacga tggaatgctt gaactccatc
     1921 gcgatcaaga gcgtcatgat catgccgaac acgctctgga ataccttgtg gtccagggga
     1981 ttga
//
LOCUS       DAFATA010000236         1963 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFATA010000236 DAFATA010000000
VERSION     DAFATA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.238"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..486)
                     /locus_tag="LGJ20_004832"
     CDS             complement(<1..486)
                     /locus_tag="LGJ20_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK2388803.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EI"
     gene            1006..1905
                     /locus_tag="LGJ20_004833"
     CDS             1006..1905
                     /locus_tag="LGJ20_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2388804.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
BASE COUNT          530 a          500 c          446 g          487 t
ORIGIN      
        1 gatctcctta tttgattcgc ccgccgctaa catggttaaa atctcccgct ggcgggcgct
       61 taacgatttg agatctttaa tgtccttttc cggcgtcgtc cgccagtctc caggcagaaa
      121 catcatcccc atcgccgcac tatttaccgc caacgcaaat gtctcgacgg ttgaatcacg
      181 aggcacaatg gccagcacat taaaatgaat aacttcctgt aaccaccgtt tattgcaatc
      241 cgtcgccgtg attaacacct taacctcagg aaactgcacc acggtttttt gcagcaacca
      301 gtagcaaaac tcaccatcct gatcgccatc gagcataact aaggcttcag ggtaactttc
      361 cagcttttgc cataactcgt ctgcctgact ggccccctga atactcactc ctggaatacg
      421 ctgctgtaaa ctgattttca ttccatgaat aaatattgac tgcctgtcaa acatgactat
      481 ttgcataact gaatctccac ctgaatacgt taaaaagact taagtagtgg aagggtatta
      541 cccgcgagaa aaaataagaa ttcgccattt ggcggtggcc attctacaga gatgacgcgt
      601 agaaaatagt taccgatata aatagttaca gctaaacgcc tgaaattaca tgtcgagggc
      661 actatttaaa acaattttga ggatttcctt atattggtgg ttagtacgca tgcaattaaa
      721 aatgaaactc cgcgaccaca agcaaaaata acaaacggca atgagacaaa aataagcaca
      781 aatagccaac acgtcctcta ttcactttaa agggaatcgc tgaaaaatac gctctgttta
      841 gggggattca cctttctcag aaagctattc cgcccttttc ctgctgagaa atcgccacat
      901 tcgtcatgac aacattgtga aacccggcat tagatgttag aaaaaacaat aaacaatgcg
      961 atatgcgcgt tactgtttcg ctgtgaacaa caatcggtca aagaaatgga taaaattcac
     1021 gcaatgcagt tgttcatcaa agtcgcggag ctggaaagtt tttcccgcgc ggcggatttc
     1081 tttgctttgc caaaaggaag tgtttcgcgc cagatacagg cactggaaca tcaacttggc
     1141 acccagcttc tccagcgcac cacgcgacgg gtcaaactca cgccagaagg catgacctat
     1201 tatcaacgag caaaagatgt gttgagtaat ctcagcgaac tggacggtct gtttcaacag
     1261 gatgccacca gtatcagcgg taaattacgc atcgacatcc cgccaggaat cgcgaaaagc
     1321 ctgttactgc cgcgcctgtc ggaatttctc tatctgcatc cgggaattga gctggaactg
     1381 agtagccatg accgtccggt agatattctt catgatggtt ttgattgcgt gatacgcact
     1441 ggcgcgttac cggaagatgg cgttatcgcc cgtcccctcg gcaaactgac catggtcaac
     1501 tgtgccagtc cgcactatct gacgcgcttt ggttatccgc aaagccccga tgatctgact
     1561 tcacacgcaa tagtgcgtta tacaccgcac ctgggtgtac atccgttagg ttttgaggtt
     1621 gccagcgtta atggcgtcca gtggtttaag tctggcggca tgttgacggt aaatagtagc
     1681 gaaaactatc tcgccgccgg tcttgccggt ctggggatta ttcagatccc gcgcattgcc
     1741 gtgcgcgaag ccctgcgtgc cgggcgcctt attgaagtgt tgccaggcta ccgtgccgag
     1801 ccgctctccc tttcgctggt ttacccgcag cgtcgggagc tttcccggcg tgtaaacctg
     1861 tttatgcagt ggctggctgg cgtaatgaaa gagcacctgg actgaccgac tatacttttt
     1921 aagaatgacc acgacagaag gacaaaagag cggatgacgc agg
//
LOCUS       DAFATA010000237         1908 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFATA010000237 DAFATA010000000
VERSION     DAFATA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.239"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     regulatory      complement(53..149)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(241..513)
                     /gene="rcnR"
                     /locus_tag="LGJ20_004834"
     CDS             complement(241..513)
                     /gene="rcnR"
                     /locus_tag="LGJ20_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBK2388805.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLIREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            634..1458
                     /gene="rcnA"
                     /locus_tag="LGJ20_004835"
     CDS             634..1458
                     /gene="rcnA"
                     /locus_tag="LGJ20_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBK2388806.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1677..>1908
                     /gene="rcnB"
                     /locus_tag="LGJ20_004836"
     CDS             1677..>1908
                     /gene="rcnB"
                     /locus_tag="LGJ20_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBK2388807.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAP"
BASE COUNT          480 a          465 c          457 g          506 t
ORIGIN      
        1 cagcaggtcg acttgcatag tttgctcctg ccataacgtg aagaagcaat gacctggtgg
       61 tccgtgactt ccctacgctg gcattatcca gatcaggtga tacgggtatt tctcagcctt
      121 cacgcagaag ggcaccccga gtcgtttggt tgcgatgata aatcgcagag gaggatggta
      181 atgtccagcg cacgcgttgt aaacgaggaa aaacggcagt tttacaatcg cgttaataaa
      241 ttatttgata tatgaatcca gcaccttcag aacgacatcc agatcttctt cacgttttag
      301 ctcatccccc tggtgaacga tgtgttccgt cagatgacct ttaatcactt cccgtatcag
      361 accgtttacc gcgccacgga tagcagcaat ctgttgtaaa actgcagcgc attcgtgcgg
      421 ctcgtcgagc attttcttga gcgccacgac ctggccctga atcttactgg cacgcgcttt
      481 cagtttctgt ttatcacgga ttgtatgaga catggcaaca cctggttaac aagaatatga
      541 aaaatcatag cactattaat ctactggggg gtagtatcag gtactggggg ggagtagaat
      601 cagattgccg aattaatact aagaattatt atcatgaccg aatttacaac tcttcttcag
      661 caaggaaacg cctggttctt catccccagc gccatcttac ttggtgcgct tcatggcctg
      721 gaaccagggc actcaaaaac gatgatggcg gcgtttatca tcgccatcaa aggcaccatt
      781 aaacaggcgg tgatgctcgg actggcagca actatttcgc ataccgcagt ggtctggtta
      841 attgcctttg gcgggatggt gatcagcaag cgctttactg ctcaatcagc agaaccgtgg
      901 ctccagctga tttccgcagt gatcattatt ggcaccgcgt tctggatgtt ctggcgtacc
      961 tggcgcggcg aacgcaactg gctggagaat atgcacgagt atgatcatga acatcatcac
     1021 cacgatcacg aagatcacca cgaccatgga catcatcacc atcacgaaca tggcgagtat
     1081 caggatgccc atgcacgagc ccatgccaat gatattaaac gacgctttga tggtagagag
     1141 gtcaccaact ggcaaatttt gttatttggc ttaacaggtg gtcttatccc ctgtccggca
     1201 gcaattaccg tgctgttgat ttgcattcag ttgaaagccc tgacactggg cgcaacactg
     1261 gtcgtcagtt tcagcattgg cctggcgtta acgcttgtca ccgtaggcgt tggcgcagca
     1321 atcagcgttc agcaggtcgc aaaacgctgg agcggattta acactctcgc taaacgcgcc
     1381 ccctattttt ccagtctgtt gattggctta gtcggtgtgt atatgggcgt acatggcttc
     1441 atgggcataa tgcgataact caatccttta ggcttgcatg ttatcttcag cccaggatga
     1501 ttcttaaatc agctatttcc gctgactctc tgctcgctaa ggatgctttt agggcatcct
     1561 ttcatttaca ctttttacga aatcatggga tcactaacaa aatatcgctt gtcagttata
     1621 ttgtatggca ggaaagatat gcgactgata ttacagatcc ccaaagtgga gagtttatga
     1681 ccattaaaaa taagatgttg ctgggtgcgc ttttgctggt taccagtgcc gcctgggccg
     1741 caccagccac cgcgggttcg accaatacct cgggaatttc taagtatgag ttaagcagtt
     1801 tcattgctga ctttaagcat ttcaaaccag gggacaccgt accagaaatg taccgtaccg
     1861 atgagtacaa cattaagcag tggcagttgc gtaacctgcc cgcacctg
//
LOCUS       DAFATA010000238         1855 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFATA010000238 DAFATA010000000
VERSION     DAFATA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.240"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            55..729
                     /locus_tag="LGJ20_004837"
     CDS             55..729
                     /locus_tag="LGJ20_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2388808.1"
                     /translation="MLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYIT
                     QDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDI
                     AKQLRLPYPIDQASSAPNVFKTFFRVLSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMD
                     TKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRT
                     SRGIRK"
     gene            726..1295
                     /locus_tag="LGJ20_004838"
     CDS             726..1295
                     /locus_tag="LGJ20_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388809.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
     gene            1521..1754
                     /locus_tag="LGJ20_004839"
                     /pseudo
     CDS             1521..1754
                     /locus_tag="LGJ20_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          608 a          335 c          394 g          518 t
ORIGIN      
        1 gtcagcacgg aaggggcaac agaatggtgc gcagaatacg atgaatgggg caacctgctg
       61 aatgaagaga acccgcatca gctgcagcag cttatccgcc tgccggggca gcagtatgat
      121 gaggagtccg gcctgtatta caaccgccac cgctattatg acccgctgca ggggaggtat
      181 atcactcagg atccgattgg gctgaagggg ggatggaatt tttatcagta tccgctgaat
      241 ccggttcagt atatagattc aatgggactg gcatcaaaat atggacactt aaataatggc
      301 ggatatggag cgagacccaa caaaccgcct acgcccgatc caagtaaatt gccggacata
      361 gcgaaacaat taagactgcc atatcctatt gaccaggcca gtagtgcgcc taatgttttc
      421 aaaacattct tcagagtatt aagcccttac gactacacac tgtattgcag gaagtgggta
      481 aaaccaaatc tgacttgtac gccacaggat gattcccagt atccagggat ggatacaaag
      541 acagcaagtg attacctgcc acagacaaat tggccaacaa ctcaattacc accaggatat
      601 acttgtgcag aaccctattt attcccagac attaataaac ccgatgggcc agcaacagca
      661 gggatagatg atttgggtga aattttagct aaaatgaaac agagaacatc gagaggaata
      721 agaaaatgaa aagagttttg ttctttttgc tgatgatatt tgttagtttt ggtgttatag
      781 ctgattgcga aatacaagct aaagatcatg attgttttac tattttcgct aagggtacta
      841 ttttttccgc gtttcctgtc ttaaataata aagccatgtg gagatggtat cagaatgagg
      901 acatcggtga gtattattgg caaacagaac tgggtacatg taaaaataat aaattcacac
      961 caagcggggc gagactctta attcgcgtcg ggtcactacg ccttaatgaa aatcatgcca
     1021 tcaaaggaac gttgcaggag ttgataaata cagcagaaaa aacggcattt ctcggcgatc
     1081 ggtttagaag ttatataaga gcgggtatct atcaaaaaaa gagcagtgat ccagttcagt
     1141 tgttggcagt ccttgataat tcaatcatgg taaaatattt taaagatgag aaaccaactt
     1201 atgctcgaat gacagcacat ctcccgaaca aaaatgagtc ttacgaatgt ttaataaaga
     1261 tacagcatga actaattcgt agtgaagaga aataaaatct cttcctgaga aaacaaatta
     1321 atcattgtga aaaattatat aactcatgga ataggtgttt attttgcgtt ccatttgcag
     1381 ggaaagatca cgtaacgcta cttttttgta ctaaataatt cgaattttaa gttcaacaat
     1441 tgagatacta cttattgtct aaagctgttt ttcatagctt atacatgatc aaatactcct
     1501 tacataaata aggtgaacaa atggaactta aaaaattgat ggaacatatt tctattaccc
     1561 ccgattacag ataagcctgg aaagtggtgc ataaattgtc agatattcta ctgttgacta
     1621 tttgtgccgt tatttctggt gcagaaggtt gggaagatat agaggatttc ggggaaacac
     1681 atctcgattt tttgaagcaa tatggtgatt ttgaaaatgg tattcctgtt cacgatacca
     1741 ttgccagagt tgtatcccag ggaaagatca cgtaacgcta cttttttgta ctaaataatt
     1801 cgcattttat gtttaaaaat tgagatattc cttattacct aaagctgttt ttcat
//
LOCUS       DAFATA010000239         1842 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFATA010000239 DAFATA010000000
VERSION     DAFATA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.241"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..494
                     /locus_tag="LGJ20_004840"
     CDS             <1..494
                     /locus_tag="LGJ20_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2388810.1"
                     /translation="GLTTYSALSADSVIKISGRVLDYGCTVSSDSLNFTVDLQKNSAR
                     QFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLKLDEGSNTASGLGI
                     EILDGNMRPVKLNDLHAGMQWISLVPEQNNILPYSARLKSTQKSVNPGLVRASATFTL
                     EFQ"
     gene            507..1010
                     /locus_tag="LGJ20_004841"
     CDS             507..1010
                     /locus_tag="LGJ20_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2388811.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAVDVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            1069..>1842
                     /locus_tag="LGJ20_004842"
     CDS             1069..>1842
                     /locus_tag="LGJ20_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2388812.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILA"
BASE COUNT          523 a          399 c          427 g          493 t
ORIGIN      
        1 tggggttaac cacctattct gctttatccg cagatagcgt tattaaaatt agcgggcgcg
       61 tcctcgatta tggctgcaca gtctcatcgg attcgcttaa ttttaccgta gatctccaaa
      121 aaaacagtgc cagacaattt ccaacgaccg gtagcacaag tccagccgtc ccttttcaga
      181 ttacgttaag tgaatgcagc aaagggacaa cgggggttcg ggttgcattt aacggtattg
      241 aggatgcaga aaataatact ctgttgaaac tggatgaggg aagcaatacg gcctccggtt
      301 taggtataga aatactggac ggaaatatgc gtccggtgaa actgaatgac cttcatgccg
      361 ggatgcagtg gatctcactg gtaccagaac agaacaatat tttgccttac tccgctcgtc
      421 tgaagtcaac tcagaagtcc gtcaatccgg gactggtgag ggcttcggca acctttaccc
      481 ttgaatttca ataatcagag tcagatatga aaagacttca caagaggttc ctgttagcta
      541 cgttttgcgc gttattcaca gcaactctcc aggccgtcga tgtcactatc actgttaatg
      601 gtcgggtagt cgctaaaccc tgcactattc aaaccaaaga agctaacgtt aatctcgggg
      661 atctttatac gcgcaatctg caacaacctg gttctgcatc tggctggcac aatattactt
      721 tgtcattaac cgattgtccg gttgaaacaa gtgcagtgac ggcaatcgtg acaggttcaa
      781 ctgacaatac gggttattac aaaaatgaag gtactgccga aaatattcag atagagctga
      841 gggatgacca ggatgcgacg ttaaaaaatg gcgatagcaa aacggttatt gttgatgaga
      901 tcactcgtaa tgcacagttt ccacttaagg caagagctat cacggtgaat ggaaacgcaa
      961 gccagggaac gatcgaggcg ctaatcaatg tgatctacac ctggcaataa cgtcgattcg
     1021 attttcttag ccttcttttt aaatcaattt ttaaccttag gatatatcat gggtaaaaca
     1081 atcagtataa aggtcctgtt cggtatttat cttctattaa tggcaggcaa agtttttgcc
     1141 ttcagttgta atgtcgatgg cgggtcaagt attggagcag gtacaacctc tgtttatgtt
     1201 aatctcgacc ctgtaataca gccgggccag aatctggttg tagacttgtc tcagcatata
     1261 agttgctgga atgattacgg cggctggtac gacactgatc atataaacct ggtacaaggc
     1321 tcagctttcg caggctccct acagtcatat aaaggatcgc tgtactggaa taatgtgacc
     1381 taccccttcc cgttgacgac aaataccaac gtattggata ttggtgataa gacaccgatg
     1441 ccattaccgt taaaactcta tattacccca gttggggcgg ccggtggagt tgtcataaaa
     1501 gcgggggaag tcattgcccg tattcatatg tataaaattg caacattagg aagcggaaat
     1561 ccgcgtaatt ttacgtggaa tattatttca aataacaatg ttgttatgcc aacaggcggc
     1621 tgcaccgttg atagtcgtaa tgtcaccgtg gatctcccgg actttcctgg aagcgcagaa
     1681 ataccccttg gcgtatattg ctcaagcgaa caaaagctct cattttatct ttcgggtgcc
     1741 accactgaca gtgcccgaca agtgtttgct aacacagcac cagatgcaac aaaagcatcg
     1801 ggagtgggtg tctctctgat gcgtaatgga aaaatactgg ct
//
LOCUS       DAFATA010000240         1837 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFATA010000240 DAFATA010000000
VERSION     DAFATA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.242"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            3..491
                     /locus_tag="LGJ20_004843"
     CDS             3..491
                     /locus_tag="LGJ20_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBK2388813.1"
                     /translation="MPVGVPVPWPSATPPTGWLKCNGAAFSVEEYPELAKAYPTNKLP
                     DLRGEFIRGWDDGRGMDTGRAILSAQGDAIRNIYGEFKTVNTENYSIWESVGSFKGAV
                     VPLNPSTNNSYFSLVRSMVTERSEGGVYPKVIGLDASRIVPTANENRPRNIAFNYIVR
                     AA"
     gene            491..1093
                     /locus_tag="LGJ20_004844"
     CDS             491..1093
                     /locus_tag="LGJ20_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2388814.1"
                     /translation="MNKAVLNNELIAIKAGDITIYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGSYKAGYAICRSADFNSWEYVPDHRGEIIYSTETGESKEITAPGDYPEN
                     ATTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQPENTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1065..1505)
                     /locus_tag="LGJ20_004845"
     CDS             complement(1065..1505)
                     /locus_tag="LGJ20_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2388815.1"
                     /translation="MQKYIFSADKNAFFPVELKIAYQESGEWPGDGIEIDDTVAAEFM
                     KEAPEGKYRGVIDGMPAWIDIPPPTHEEQIAAAELEKQQLINQVNEYINSKQWPGKAA
                     IGRLKGGELAQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
BASE COUNT          495 a          437 c          436 g          469 t
ORIGIN      
        1 cattgcctgt tggcgtacct gttccgtggc cctcagccac tccgccaaca ggctggctga
       61 aatgcaacgg agcagctttt tctgttgaag aatacccgga actggcaaag gcttacccga
      121 caaataaatt gcctgattta cgtggtgagt ttattcgtgg ctgggatgac ggacgtggta
      181 tggatacggg gagggcaata ttatccgctc agggcgatgc catacgtaat atctatggtg
      241 agttcaagac tgtaaacacc gaaaattatt caatatggga atcagtaggc tcgtttaagg
      301 gggcagtagt gcctttgaac ccctcaacga acaatagtta tttctcctta gtcagaagta
      361 tggtgacaga aaggtccgag ggcggtgttt acccaaaagt gattggcctt gatgcttcaa
      421 gaattgttcc aactgcaaac gaaaaccgtc ctcgtaacat tgcctttaat tatatcgtga
      481 gggctgcctg atgaataaag ccgtattaaa taacgaactc attgccataa aagcgggaga
      541 cattaccatt tataattatg atggtgaaac gcgggaatat atttccacat caactgaata
      601 tcttgcggtt ggcgtcggta ttccggcatg ttcctgttta gatgctcctg gctcatataa
      661 agctggttat gcaatctgcc gttctgcaga ttttaactca tgggaatatg tgccagacca
      721 tcgcggtgaa atcatctata gcaccgaaac aggagaatcg aaagaaatca cagctccggg
      781 tgattatcct gaaaatgcaa ccactatcgc cccgttaacg ccatacgata aatgggatgg
      841 tgagaaatgg gtgacggata ccgaggcaca gcatagcgcc gcagtagaag cggcagaagc
      901 acagcgtcag tcgctgattg atgctgcaat ggcttccatc agtctgattc agctgaaatt
      961 acaggccggg cggaagctga cgcagccaga aaacacccga cttaacgctg tgctggatta
     1021 tattgacgcg gtgacggcaa cagataccag cacagcgcca gacgtcatct ggcctgaact
     1081 gccggaggcg taggccattc aatatctggc gcaccggaag tatcgaccag ttccagtgcg
     1141 tccagataat ccagccacaa attatattgc gccagttccc cacctttcag gcgaccaatc
     1201 gccgctttac caggccattg cttactgttt atgtattcgt tgacctgatt aatcaattgc
     1261 tgcttttcca gttcggctgc ggcaatttgt tcctcatgag ttggcggtgg aatatcaatc
     1321 catgcaggca ttccgtcgat gacacctctg tattttcctt ctggtgcttc cttcataaat
     1381 tcggcggcaa cagtgtcgtc aatttcgatt ccatcaccgg gccattcgcc ggattcctga
     1441 taagcgattt taagctccac agggaaaaac gcatttttat cggcactgaa aatatatttc
     1501 tgcatttcta ccgtcctatc gaaacataac tgaatctgta ttgctgtgag atatcactgg
     1561 ttgccacacg ccacgctgaa ttactgatat gttcaaaatt tacagacaaa acctgcgggg
     1621 caggattcga cgggtctgac tgaacggcat cagacataac actgactgaa accatcggct
     1681 gattagggaa tggtataggg aagtgtccac tgataaagcg ggtcgtgttt cctgaaaaag
     1741 tgccaaactg aacaatatat ccacctggta gcctgaacca tcccgaacca gaagcgaatg
     1801 ctcccatatc cggtatctga ttatctcctg tgcccac
//
LOCUS       DAFATA010000241         1817 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFATA010000241 DAFATA010000000
VERSION     DAFATA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.243"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..852
                     /locus_tag="LGJ20_004846"
     CDS             <1..852
                     /locus_tag="LGJ20_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388816.1"
                     /translation="SGISQVFNWFKSLLSPMESSKETLDKCTSAGEIFGNVLGGALQL
                     VLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQDKVALLQGDLAKINP
                     PKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVEETKKRIGPGDIVFK
                     NLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPVVAASSRPVAEAIRS
                     PVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGEMVRAEMERRARAGR
                     GSFYDKD"
     gene            865..1353
                     /locus_tag="LGJ20_004847"
     CDS             865..1353
                     /locus_tag="LGJ20_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388817.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1382..>1817)
                     /locus_tag="LGJ20_004848"
     CDS             complement(1382..>1817)
                     /locus_tag="LGJ20_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2388818.1"
                     /translation="DTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSP
                     MGRFFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKN
                     GHDRKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
BASE COUNT          424 a          451 c          494 g          448 t
ORIGIN      
        1 agtgggatca gccaggtgtt taactggttt aaatcgctgc tgtcaccgat ggagtccagc
       61 aaggaaactc tggataaatg taccagtgct ggcgagatat tcggtaacgt tcttggcggt
      121 gcgttacaac ttgttctgac acctgcaaaa atgttgctgg atacgctggc gtggatactt
      181 gaaaagcttg gtgtgcttcc ggatgaagcg gaaagggcgc gcaagaaaat cgaagacgca
      241 cagcgtgcgg ccattcttca ggacaaggtt gccttgcttc agggggacct ggcgaaaatc
      301 aatccgccga agcctgtgga aaatggcaat ggcaccggag gtgataaccc caaagacaat
      361 aaaccgctca cagacagcaa taccggtacg ctgcgcagac tcagcaaaat tgctgataac
      421 acaggtaagc tggttgagga gacgaaaaaa cgcattggcc ccggcgatat tgtctttaaa
      481 aacctgcccc gagcacttgc tgttcgtggg gagtggcagg agcggaagat tgcgcaggtc
      541 agtaagcctg cccccgcaat taatatcaca cccgtggtcc cggctccgct gcctccggcg
      601 ctggtccctg ttgttgcggc cagctcccgc ccggtggcgg aggctatacg atctccagtg
      661 gcatcagttc ctgtaacttc ccgtaaccgg gagcctgttg cctccggatt tggtggtgaa
      721 attcatgttc atctgcataa cgttgttacg cagaatcccc gcgaactggc gaaactggtc
      781 ggtgaaatgg tcagggcaga aatggaacgg cgcgcccgtg ccgggcgtgg cagtttttac
      841 gataaagatt gaggagtcat ggccatgatg atgatctacg gcatgtttgt ttttgagctg
      901 cgcacgctgc cgcatcagca gttacagcaa aacaaaagct ggcggcatgt gaaaaatgaa
      961 cgcgttaacc gttcagcaag ctggcagtat atcggtgcag gtgatgatcg catcgttctt
     1021 tctggtgtgc tttatcctga aattacaggt ggcgaagtgt cgctgtcgct gctgaccacg
     1081 caggcgtata caggacgacc ctggcctttg attgatggcg tcgggcagat ttacggcatg
     1141 tatgtcctga ctgaaacgaa tacgacccgc tccgagtttg atcgctacgg taaggcgaaa
     1201 aagatagaat tttcactgac tcttgaacgc tgtgatgagg atttgcggga gcgcctgcaa
     1261 tcctcatcgt tcagtgatat gctgtccggc ttcaaagata aggtgacatc atctcttaac
     1321 agcgcggcca gttcagttaa agggctgttc tgatttaaca caaaaaccgc taatggtcag
     1381 attagcggtt ttcattttcc tgagtctgcc cggttgtttc ttcagtctgt atatcgccta
     1441 cagggtgata acgataaatc gtcgatgtgc caatgccgta aattattgcc agttgttttc
     1501 tgtcgtgccc gtttttgatt agccttgcta tttgctcatg ctgttctttt gtcagcttcg
     1561 gtcgacgtcc gcctgtgcgc ccccttgcgc gcgctgccgc cagcccggcc agtgtacgtt
     1621 caacaattaa ttcacgttcc atttcagcca gggcacccat cacgtggaag aaaaaacgcc
     1681 ccattggaga agatgtgtct atgctgtcgg tcagactgcg aaaattaatc cctcgctccc
     1741 gtagttcccc gacgagagaa atcagatgtt tcatgcttcg cccgaggcga tccagtttcc
     1801 agacaaccag cgtgtca
//
LOCUS       DAFATA010000242         1790 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFATA010000242 DAFATA010000000
VERSION     DAFATA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.244"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..751)
                     /gene="eamA"
                     /locus_tag="LGJ20_004849"
     CDS             complement(<1..751)
                     /gene="eamA"
                     /locus_tag="LGJ20_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBK2388819.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLL"
     gene            946..>1790
                     /locus_tag="LGJ20_004850"
     CDS             946..>1790
                     /locus_tag="LGJ20_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388820.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTISVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTL"
BASE COUNT          473 a          474 c          390 g          453 t
ORIGIN      
        1 ccagtaacga taacggtgca acccgccagg tttcatagcg tcccagtaac gtcccccaga
       61 tcccataacc aacaattgtc gccacaaacg ccagatacat cagcgacaag atggtggtca
      121 tatcaatagt aaccagactg tgaatcatgg atgcggaacc atcgagaatc agcgaggcaa
      181 caaagaaggg aatgattggg attaaagcgc tccagattac cagcgacatc accgccggac
      241 gcgttgagtg cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg
      301 cagccagggt caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga
      361 ttaacaccag tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgcaatc
      421 gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca
      481 gcgaagccag tccagcaggc ataccgaagt taatggcaca aaaaagaaaa gcaaactgcg
      541 caaaactgat ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtgcga
      601 caaaaaagat agccggaaaa gcgaccagca taaagcgcaa accggccagc atcagcggtg
      661 gcatgttatg aagccccact ttgatgacca caaaatttag cccccatacg accactacca
      721 gtagcgccaa caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta
      781 agcaatcaac ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac
      841 gacggcatta atgcgcaaat aagtcgctat acttcggatt tttgccatgc tatttcttta
      901 catctctaaa acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta
      961 cgacgctcta ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct
     1021 acgctgccat ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc
     1081 ggttatgcga tgacaattgc gctcactatt agcgtcgttt ttagcctcgg ttttggtatc
     1141 ctggcggata agttcgacaa gaaacgctat atgttactgg caattaccgc cttcgccagc
     1201 ggttttattg ccattccttt agtgaataac gtaaagctgg tggtgctctt ttttgccctc
     1261 attaactgcg cctattctgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt
     1321 tcgtccacca gcaaaacgaa aatcttctca atcaactaca ccatgctaaa cattggctgg
     1381 accatcggtc cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct gcccttctgg
     1441 ctggcagcta tctgttccgc gtttcccatg cttttcattc aaatttgggt aaagcgcagc
     1501 gagaaaatca tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat
     1561 aaagcactgt tgtggtttac ctgctctggt tttctggctt cttttgtaag cggcgcattt
     1621 gcttcatgca tttcacaata tgtgatggtg attgctgatg gggattttgc cgaaaaggtg
     1681 gtcgcggttg ttcttccggt gaatgctgcc atggtggtta cgttgcaata ttccgtgggc
     1741 cgccgactta acccggctaa catccgcgcg ctgatgacag caggcaccct
//
LOCUS       DAFATA010000243         1788 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFATA010000243 DAFATA010000000
VERSION     DAFATA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.245"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            107..>1788
                     /locus_tag="LGJ20_004851"
     CDS             107..>1788
                     /locus_tag="LGJ20_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBK2388821.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          551 a          409 c          364 g          464 t
ORIGIN      
        1 tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct
       61 tcgtaaaatt acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt
      121 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
      181 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
      241 agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat
      301 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
      361 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
      421 acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacggt ttactaacgg
      481 ctatgtggca cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc
      541 cgcccgcttt ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac
      601 tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca
      661 cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga
      721 caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt
      781 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg
      841 tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
      901 tgttgatcgt gccaaaacac ttgaccagcc atttatgctt tacctggctt ataatgctcc
      961 gcacctgcca aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag
     1021 tcaaacagca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     1081 tctcgaacaa ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga
     1141 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     1201 tcagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca
     1261 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     1321 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     1381 gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca
     1441 ctggtttgac gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca
     1501 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     1561 gagaaataac gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa
     1621 actgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     1681 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa
     1741 tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaata
//
LOCUS       DAFATA010000244         1760 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFATA010000244 DAFATA010000000
VERSION     DAFATA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.246"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..965)
                     /gene="secA"
                     /locus_tag="LGJ20_004852"
     CDS             complement(<1..965)
                     /gene="secA"
                     /locus_tag="LGJ20_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK2388822.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTR"
     gene            complement(1027..1539)
                     /gene="secM"
                     /locus_tag="LGJ20_004853"
     CDS             complement(1027..1539)
                     /gene="secM"
                     /locus_tag="LGJ20_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBK2388823.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
BASE COUNT          405 a          436 c          466 g          453 t
ORIGIN      
        1 cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg
       61 gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag cagttcttca
      121 atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc caccgagaag
      181 tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag gtgcggaata
      241 attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc
      301 ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt
      361 ttacgctgta cacgttcttc agggctgaac gccatattgt cgcgcaggta gtcaaagccg
      421 tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct
      481 ggcatgcccg gcaggttgat accgacagtc aggccaagga attcaaacag cggacggttg
      541 ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac gcctttaccg
      601 gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc
      661 atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga
      721 cgcataccaa agacgcgctt acttgcctca cgtaccacgg cgaaagcttc cgggatcaga
      781 ttttccagca cttcgccttt ttccagacgt gcacgaaact ctgcggtttt ccctttcagt
      841 tcttcgtcgg agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg
      901 cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa caatttgatt
      961 agcataataa aatctcaaac gccccgcgtt gcggagttaa taaaatgaag taaaggttta
     1021 ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac
     1081 gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat aacccttttc
     1141 agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg caagatgttg
     1201 cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca ttgcgaaaga
     1261 aagatgacgg attaccgtgc gaatggcatg ttgatgccag taatcaacgg aatagttcga
     1321 attcgggcgg cgtgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga
     1381 aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag
     1441 cgcaggcaaa cctaaactcg ccgcaaccat ccctaataag agatgcggcc agaagtagcg
     1501 tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgta
     1561 ttcaaacgta tttagcgcac aaatcttatc attaaagcct gcagtccaca gcacgaatga
     1621 agataagttg tgcggaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatagctct
     1681 tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca gatttgccag
     1741 tcgaatttta tacgacagta
//
LOCUS       DAFATA010000245         1731 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFATA010000245 DAFATA010000000
VERSION     DAFATA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.247"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..861
                     /gene="ytfQ"
                     /locus_tag="LGJ20_004854"
     CDS             <1..861
                     /gene="ytfQ"
                     /locus_tag="LGJ20_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBK2388824.1"
                     /translation="SESGWRAAETNVAKSEAEKRGITLKIADGQQKQENQIKAVRSFV
                     AQGVDAIFIAPVVATGWEPVLKEAKDAEIPVFLLDRSIDVKDKSLYMTTVTADNILEG
                     KLIGDWLVKEVNGKPCNVVELQGTVGASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFT
                     RSKGKEVMESFIKAENNGKNICMVYAHNDDMVIGAIQAIKEAGLKPGKDILTGSIDGV
                     PDIYKAMIDGEANASVELTPNMAGPAFDALEKYKKDGTMPEKLTLTKSTLYLPDTAKE
                     ELEKKKNMGY"
     gene            1171..>1731
                     /locus_tag="LGJ20_004855"
     CDS             1171..>1731
                     /locus_tag="LGJ20_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK2388825.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGL"
BASE COUNT          470 a          446 c          464 g          351 t
ORIGIN      
        1 tcggaatccg gctggcgcgc tgcagaaacc aatgtggcga aaagtgaggc cgaaaaacgc
       61 ggaattacgc tgaaaattgc cgatggtcag caaaagcagg aaaaccagat taaagcggta
      121 cgttccttcg tcgcgcaagg ggtggatgcg atctttatcg ctccggtggt cgcgacaggt
      181 tgggaaccgg tattaaaaga ggcgaaagat gccgaaatcc cggtcttctt gctcgaccgt
      241 tccatcgatg tgaaagacaa atctctctat atgaccaccg ttactgccga caacatcctc
      301 gaaggtaagt tgatcggtga ctggctggta aaagaagtga atggcaaacc atgcaacgtg
      361 gtggagctgc agggcaccgt tggggccagc gtcgccattg accgcaagaa aggctttgcc
      421 gaagccatta agaatgcgcc aaatatcaaa attatccgct cgcagtcagg tgactttacc
      481 cgcagtaaag gcaaagaagt catggaaagc tttatcaaag cggaaaacaa cggcaaaaac
      541 atctgcatgg tttacgccca taacgatgac atggtgattg gcgcaattca ggcaattaaa
      601 gaagcgggcc tgaaaccggg caaagatatc ctcacgggtt ccattgacgg tgtaccggac
      661 atctacaaag cgatgattga tggcgaagcg aacgccagtg ttgaactgac gccgaatatg
      721 gcgggacccg cttttgacgc gctggagaaa tacaaaaaag acggcaccat gcctgaaaag
      781 ctgacgttaa ccaaatccac cctttacctg cctgataccg caaaagaaga gttagagaag
      841 aagaaaaata tggggtattg agggttgcta tgcctgatgc cgattcgtag gccggataag
      901 gcgctcgcgc cgcatccggc ggtggtgcgc tgaagcctga tgcgacgctt accgcgtctt
      961 atcatgccta cgagttcggt gctctgtgta ggccggataa ggcgttcacg ccgcatccgg
     1021 cagtggtgca acaccggact taacaccgtg cacaatctgt cccctcgccc cttcggggag
     1081 agggttaggg tgagggggac aaacggcgca gctcttaaca tcaggcacga tctattccca
     1141 gacttttaga gcgtggctaa gggggaaacc atgacgaccg accaacacca ggaaatcctc
     1201 cgcaccgaag gattaagtaa atttttcccc ggcgtcaaag cgttagacaa cgttgatttc
     1261 agcctgcgcc gtggcgaaat catggcgctg ctcggtgaaa acggggcggg aaaatcgacg
     1321 ctaatcaaag cattaaccgg tgtataccac gccgatcgcg gcaccatctg gctggaaggc
     1381 caggctatct caccgaaaaa taccgcccat gcacaacaac tcggcatcgg aaccgtttat
     1441 caggaagtca acctgctacc caatatgtcg gtcgctgata atctattcat aggtcgcgaa
     1501 cccaaacgtt tcggctttct acgccgcaaa gagatggaaa agcgtgccac cgaactgatg
     1561 acatcttacg gtttctccct cgacgtgcgt gaaccgctta accgcttttc agtcgcgatg
     1621 cagcaaatcg tcgccatttg ccgggctatc gatctctctg ccaaagtgct gatcctcgat
     1681 gaacccaccg ccagtctcga cactcaggaa gtagaattac tgtttggcct g
//
LOCUS       DAFATA010000246         1717 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFATA010000246 DAFATA010000000
VERSION     DAFATA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.248"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..987)
                     /gene="nrfB"
                     /locus_tag="LGJ20_004856"
     CDS             complement(<1..987)
                     /gene="nrfB"
                     /locus_tag="LGJ20_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBK2388826.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSR"
     gene            complement(1256..>1717)
                     /locus_tag="LGJ20_004857"
     CDS             complement(1256..>1717)
                     /locus_tag="LGJ20_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HBK2388827.1"
                     /translation="SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRG
                     NAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          436 a          411 c          416 g          454 t
ORIGIN      
        1 gcgagatttt tgtcgaactg cagtcgaaaa ggtatcgggg aaatattcgc gcacgcagat
       61 catgtttgac gtccgtgcgt gctgtaaaaa tttgcgctgc tcgcgttctt tggcttcgtc
      121 caccaccgga aaacggacaa aaatttccgt catacctttc tctttcaggc ggaaaccaat
      181 gtcgtaatct tcagtcagac tctgcacgtc gaaagcaata ccgtcaccgt cagctaacag
      241 agcggtcacg gcgcggcggc tgaaacaggt gccgacgcct gcgctgggca cttgtccggc
      301 gagggcttca cgcaccggaa catctttgcc atgcagctct gaaaactcat caatgtaagt
      361 catgctggtg aagtgcgtcc attcgcgttc gaacggatac accgggatct gaatcagatc
      421 tttacgctcg accagatagt tgaacagacg caattccatc ggtgaaatca catcttcggc
      481 gtcatgcaga ataaaaccag caaaagcgaa attggcgcta cgttcaaact gggtgatggc
      541 gtccagcacg ttgttcagac agtcggcttt gctggtggga ccaggacgcg cgcagactac
      601 cttatgcacg ttcggaaagc gagcgcacac ttcgtcaaca tcacgttgag tatcggggtc
      661 gttggggtag gtgccaacaa agatatgata gttttcgtag tcgagcgtgg tcgccgccag
      721 ctcggccata ttgccgatga cgcccgtttc attccacgcc ggaaccataa tcgctaacgg
      781 tttttcatct ggtttataca gttcgcggta actcattcgc gggtagcggc gataaacact
      841 caacttgcgt ttaatgcggc gtacccagta gacgacatca ataaaaaaat cgtccagccc
      901 gctgatgaac atgatgaccg ctaacgttat cgcgattact tttaagccgt agagccaggt
      961 tgcaaaaaca tcaagaagcc agtccacaca aaaaccttac attaacgctg gttatgttta
     1021 gggtggcgta tattaaggtt ttttatgaat tgtgacagct ttttaccatt aataggtatg
     1081 actattgcgg cacgttattt ttacactggt tataaaagtt gccgtttgcg tagaatgtag
     1141 acaaaagcat ggtgtcaagg aggggaggtt aacgtcatag tgaaaaggag cggtggcacc
     1201 gagcaggtca taatgtagtg gaagtgcctg gaggaggtga gcaggcaggg caagattacg
     1261 aaagcccgct ccccgcaagg actgacgcga gatagtttct gtccatggct gcttttcgca
     1321 tcttacgtct taaccctgcc ttgaatacct tatcattcgt taaaatatta atagcgatgt
     1381 gccgtatccc tgaaaataat tctgcggcgt ttcctcttct tattttgcag tcgtcttcat
     1441 tcattaccac gtccagacgc cagtgcagct tattctccac gtgccagtgg tttcggattg
     1501 ctgtggcgaa cttctctgcg gttaaatcag cagaactgat ataatatctg accgtcattt
     1561 cgggctcttt cttttgttct gctattattg accgaaagga gactgccacg cataatttct
     1621 tcagaccttt ccattcaaac gtgaaatcaa taagttcatc agggacatcg caaacaatat
     1681 gaagacggat ttcttctctg ccgtgactct tttcact
//
LOCUS       DAFATA010000247         1711 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFATA010000247 DAFATA010000000
VERSION     DAFATA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.249"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..331)
                     /locus_tag="LGJ20_004858"
     CDS             complement(<1..331)
                     /locus_tag="LGJ20_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBK2388828.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWKSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGT"
     gene            complement(343..1167)
                     /locus_tag="LGJ20_004859"
     CDS             complement(343..1167)
                     /locus_tag="LGJ20_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388829.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            complement(1167..>1711)
                     /locus_tag="LGJ20_004860"
     CDS             complement(1167..>1711)
                     /locus_tag="LGJ20_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBK2388830.1"
                     /translation="AAAGLFKIAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLL
                     GVKSGLLAGGIGILVALAVFIVGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQ
                     ESLLLMAGKQRAFLVAQTIASIGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIP
                     TLKAFFQRHSLLYNAAGEKS"
BASE COUNT          468 a          441 c          388 g          414 t
ORIGIN      
        1 cagtaccaaa gatggcatca tcggtgttca caatggtggt gggctgaaaa cgatcctgcg
       61 gaaagtttgc cgcttcaacc gctttaatta acagtgagtt ctttttcttc gccgacttac
      121 gcccggcgat atactgaata atgcggactt ttcccgctaa ttgtgtgctt ttccactttt
      181 ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc gccttcctgg atataaacag
      241 gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac acccggggct aagagcatga
      301 taatcaacgc aataatcaaa cgagatgaca tcattgctcc ggctatagcg gaaaacgcga
      361 aatcagcagc tctaagcgct gacgaatatc gctatccagc gccaacggtg catcatacga
      421 ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt ttatctccca tcaggccatt
      481 aatggcggcg aggaaacggg aaatctgtaa cgcctcttta ttttcaggat caagcgatct
      541 taaggtgtcg taataagaaa cctgttggct gatgattttt cgcacatcat gaaattccct
      601 gccggttagt cgcggtttgg caataatctc tttcagggta tgcatttgct tttgccgata
      661 ggcaataaaa ctgcttacgc tttctaactg gctatggcgt aatgaatagt tcaccaacga
      721 ccagaccgga gagcttaaat aaatccgtaa caggttgccg taatacgaca caatattttt
      781 cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa acggtcgttt tgccgaataa
      841 aaatccggcc tgatgcttct ttaaatacag ccgttgcgca aaacgcaggt gcttgtagcg
      901 cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc agctcatcgc ctttggcttg
      961 tttgcgaact aaacgcagta attcctgacg atcaacgcca ttgagccaga attgcaggca
     1021 taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt agtgcaatag tcggcggcaa
     1081 actaacctgt gcatcaacga catcatcttc ttcaatcgca acaaatagac gctcgctcgc
     1141 ctcaagcggg aaatattttc tcatcatcag gatttctctc cggctgcgtt ataaagcaag
     1201 gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg agagcgccac gtccagacat
     1261 tgcccaccga agtaagcaaa cgctgctccc agcacaccaa acagatgaca gaacatgaac
     1321 agcaatacga tgtagccgat tgaggcgatg gtttgcgcca cgagaaaagc gcgctgtttt
     1381 cccgccatca atagcaatga ctcctgaggg aagcccagca tcgaaatcac aattgcgccc
     1441 agcatcacct gaatcaggtc atacgcttcg agatacttaa cgccaaacac cagcgaaatg
     1501 agcggcttgc cgacgataaa caccgcgagc gccaccagaa taccgattcc gcccgccagt
     1561 aaaccggact tcacacctaa caaccacggt ctggtggtgc gcggatctaa acgcatcacc
     1621 tctgggtaaa aacttttacc cagcaaaccc gctggcgttc cggcggcgtc gaagaatgtc
     1681 atggcgattt taaataaccc ggcggcagcg g
//
LOCUS       DAFATA010000248         1627 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFATA010000248 DAFATA010000000
VERSION     DAFATA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.250"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..467)
                     /locus_tag="LGJ20_004861"
     CDS             complement(<1..467)
                     /locus_tag="LGJ20_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK2388831.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLP"
     gene            672..1538
                     /gene="yghU"
                     /locus_tag="LGJ20_004862"
     CDS             672..1538
                     /gene="yghU"
                     /locus_tag="LGJ20_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBK2388832.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          402 a          415 c          438 g          372 t
ORIGIN      
        1 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
       61 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
      121 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
      181 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
      241 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
      301 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
      361 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
      421 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
      481 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
      541 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
      601 aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta ccactcagaa
      661 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
      721 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
      781 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
      841 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
      901 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
      961 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc cgcgtgtttg
     1021 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     1081 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     1141 tcggcggtgg ttttggtcac ttttaccatt acgcgccgat gaaaattgag tacgccatca
     1201 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     1261 ataagtttat tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     1321 gcaatgtggt gttaggcggc gtgtatgatg ccgcagagtt tcttgatgcg ggcagttata
     1381 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     1441 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     1501 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     1561 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     1621 cgacgct
//
LOCUS       DAFATA010000249         1622 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFATA010000249 DAFATA010000000
VERSION     DAFATA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.251"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..355
                     /locus_tag="LGJ20_004863"
     CDS             <1..355
                     /locus_tag="LGJ20_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBK2388833.1"
                     /translation="GLGGLITEFFINVANKDTFPVMTFFSSALINFAVPSGGGHWVIQ
                     GPFVIPAAQALGADLGKSVMAIAYGEQWMNMAQPFWALPALAIAGLGVRDIMGYCITA
                     LLFSGVIFVIGLTLF"
     gene            386..1570
                     /gene="atoB"
                     /locus_tag="LGJ20_004864"
     CDS             386..1570
                     /gene="atoB"
                     /locus_tag="LGJ20_004864"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HBK2388834.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGLVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKNLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
BASE COUNT          384 a          400 c          459 g          379 t
ORIGIN      
        1 cggtctgggc ggactcatta ccgaattctt catcaatgtt gcgaacaaag acaccttccc
       61 ggtaatgacc ttttttagtt ctgcactgat taacttcgcc gttccgtctg gcggcggtca
      121 ctgggttatt cagggacctt tcgtgatacc cgcagcccag gcgctgggcg ctgatctcgg
      181 taaatcggta atggcgatcg cctacggcga gcaatggatg aacatggcac aaccattctg
      241 ggcgctgcca gcactggcaa tcgccggact cggtgtccgc gacatcatgg gctattgcat
      301 cactgccctg ctcttctccg gcgtcatttt cgtcattggt ttaacgctgt tctgacggca
      361 accctacaga cagaaggaat aaaaaatgaa aaattgtgtc atcgtcagtg cggtacgtac
      421 tgctatcggt agttttaacg gttcactcgc ttccaccagc gccatcgacc tgggggcgac
      481 agtaattaaa gccgccattg aacgtgcaaa aatcgattca caacacgttg atgaagtgat
      541 tatgggtaac gtgttacaag ccgggctggg gcaaaatccg gcgcgtcagg cactgttaaa
      601 aagcgggctg gcagaaacgg tgtgcggatt cacggtcaat aaagtatgtg gttcgggtct
      661 taaaagtgtg gcgcttgccg cccaggccat tcaggcaggt caggcgcaga gcattgtggc
      721 ggggggtatg gaaaatatga gtttagcccc ctacttactc gatgcaaaag cacgctctgg
      781 ttatcgtctt ggagacggac aggtttatga cgtaatcctg cgcgatggcc tgatgtgcgc
      841 cacccatggt tatcatatgg ggattaccgc cgagaacgtg gccaaagagt acggaattac
      901 ccgagaaatg caggatgaac tggcgctaca ttcacagcgt aaagcggcag ccgcaattga
      961 gtccggtgct tttacagccg aaatcgtccc ggtaaatgtt gtcactcgga agaaaacctt
     1021 cgtcttcagt caagacgaat tcccgaaagc ggattctacg actgaagcgt taggcgcatt
     1081 gcgcccggcc ttcgataaag caggaacagt taccgccggg aacgcgtctg gtattaacga
     1141 cggtgctgcc gctctggtga ttatggaaga atctgcggcg ctggcagcag gccttacccc
     1201 cctggctcgc attaaaagtt atgccagcgg tggcgtgccc cccgcattga tgggtatggg
     1261 gctagtacct gccacgcaaa aagcgttaca actggcgggg ctgcaactgg cggatattga
     1321 tctcattgag gctaatgaag catttgctgc acagttcctt gccgttggga aaaacctggg
     1381 ctttgattct gagaaagtga atgtcaacgg cggggccatc gcgctcgggc atcctatcgg
     1441 tgccagtggt gctcgtattc tggtcacact attacatgcc atgcaggcac gcgataaaac
     1501 gctggggctg gcaacactgt gcattggcgg cggccaggga attgcgatgg tgattgaacg
     1561 gttgaattaa tcaataaaaa cacccgatag cgaaagttat cgggtgtttt cttgaacatc
     1621 ga
//
LOCUS       DAFATA010000250         1599 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFATA010000250 DAFATA010000000
VERSION     DAFATA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.252"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(72..956)
                     /gene="xapR"
                     /locus_tag="LGJ20_004865"
     CDS             complement(72..956)
                     /gene="xapR"
                     /locus_tag="LGJ20_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HBK2388835.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(997..1155)
                     /locus_tag="LGJ20_004866"
     CDS             complement(997..1155)
                     /locus_tag="LGJ20_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HBK2388836.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1208..>1599)
                     /gene="xapB"
                     /locus_tag="LGJ20_004867"
     CDS             complement(1208..>1599)
                     /gene="xapB"
                     /locus_tag="LGJ20_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HBK2388837.1"
                     /translation="AWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFV
                     EQEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYA
                     LFLAVIFFFGFKYNHDPEKIKHRAVAH"
BASE COUNT          475 a          422 c          329 g          373 t
ORIGIN      
        1 tgcgatttgc gaaattgcct gatgcgcttc acttagcaga cgactatttc cggcaattcc
       61 tgtctcctca cctactgtgt caatgcagcc aacagcttaa ccatcgcggg cgtcacctgc
      121 tgtgtttcat aaacaatata taaatctgca gggatgcgct gtttgagcgg acggaaaatg
      181 acacctggcc agttcatttg tgcgtagctg tccgctatca atgtgatacc aatacccata
      241 ctgaccatgg cgagtaccgt ttgcggttca ttaacttcgc gaataacaac cggtgaaaat
      301 cccacctgct ggcaaactcg ctgcaaaaaa tcccagtcag tgtacacggg cggcattgta
      361 acaaaatact cgtcacgtag cgcttccagc ggaacggtgg aaaatgatga gagatgatgc
      421 tcttcaggca tcgccaccag aaacgccgat tcatgcaacc gtaagctggt aaaacctgtc
      481 ggtggttctg tcgccattcg ccagatcccg gcatcgagtt cacgtcgttc cagcagggcc
      541 atttgcatcg cgggcatctt ttcgcgaaaa agaacttcaa cgttaggatt ttccctgagg
      601 aatcgccgca taaccgggcg catccgtccc cacatagccg ttcccactac gccgagttca
      661 atccgccctg cttctccccg acctatttgt tcaacccgag ccaatacatt attagcattc
      721 accagcaatc gacgcgattc ttccatcaag attttgcccg cgtgtgtcag tacgacgctg
      781 cgcgaatggc gaataaaaag ctgcgtgccg agttgatttt ccagctcttt aatatgaata
      841 ctgagcggag gctgagacat atttaaacgc gctgctgcgc ggccaaaatg caactcttcc
      901 gctacggcaa gaaaataacg gagcaactta agatctgttc tgtatacgcg ttccataatt
      961 aagtgctcca tataataaat acatcgtatt aaattatcat ggcataaagt atttcaccac
     1021 cagatatccg acatacataa acacaatgga taatatacct ggtaatacat ttggtacagg
     1081 aataggcaag cgcacgattg taaacaaagc accaatgcca aaaccaacgc cggaagaaaa
     1141 taaaatatct ttcactaatt aacctttatc ataaaagcag ctctgaagag cagagctgcg
     1201 aatcctttta atgagccacc gctcgatgct ttatcttttc agggtcatga ttatatttaa
     1261 acccaaagaa aaatatcact gcgagaaaaa gagcatatcc tgcaaacacc agccagatag
     1321 tttgccagtc ttttacgcca tccaccgaaa agtaatctac tgccatgcca ctcagaatcg
     1381 agccaaccca tgcgccgaca ccatttacca tggtcataaa gagcccctgc gcgctggcac
     1441 gaatgctgga atcaacttcc tgttcgacaa ataccgaacc agaaatattg aagaaatcga
     1501 atgcacagcc ataaacaatc atcgacagca gcagcaaaat aaatccggtt gttgacggat
     1561 cgccataggc gaagaagcca aagcgcagcg tccaggcca
//
LOCUS       DAFATA010000251         1590 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFATA010000251 DAFATA010000000
VERSION     DAFATA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.253"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..502
                     /gene="xylR"
                     /locus_tag="LGJ20_004868"
     CDS             <1..502
                     /gene="xylR"
                     /locus_tag="LGJ20_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HBK2388838.1"
                     /translation="LSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVR
                     VIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGE
                     TIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDV
                     NSEVML"
     gene            complement(610..1434)
                     /locus_tag="LGJ20_004869"
     CDS             complement(610..1434)
                     /locus_tag="LGJ20_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBK2388839.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSGSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
BASE COUNT          406 a          380 c          367 g          437 t
ORIGIN      
        1 tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg gctatcaggc
       61 ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc gaattttggt
      121 cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga ccgatcccgc
      181 cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta aagtggatca
      241 ggtactggat gcggtcggga tctcgcgctc caatcttgag aagcgtttta aagaagaggt
      301 gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc gcagtctgct
      361 gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc catcgctgca
      421 atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt atcgcgatgt
      481 aaatagcgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc agggaactca
      541 gcacagccac ctgatgcgag caaaagcctt atccgaccaa caaatcagat aaggctcaag
      601 gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca ttgcgaacag
      661 gtagttatta taactcttcc ctttggtcga gtagcccttc agcttgtgaa tcatcgctgt
      721 ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga acgaagagta
      781 agccggatgc gtattgaggt tagtgacata ggcgctcacc gattctttga cagaactaaa
      841 ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca tgcatttcat
      901 gccgaaaaga ttgttgttgt tgcgcgccag cttcgacgtt ccccaaccgc tttctgctgc
      961 agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca aggtattcca
     1021 cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga tgtctttcag
     1081 acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca gccagttacg
     1141 ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc ggagaaacgc
     1201 ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac cactctttgc
     1261 actattgcga gaatactctt gtttactgct tacctgttta ttactagctt tagttaagtg
     1321 ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg aaaacaccag
     1381 agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa tcatcaggtc
     1441 tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga gagttgaatc
     1501 tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa atctgaaact
     1561 atgtcacgtg ttaacgattc agattggcgc
//
LOCUS       DAFATA010000252         1563 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFATA010000252 DAFATA010000000
VERSION     DAFATA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.254"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..357
                     /locus_tag="LGJ20_004870"
     CDS             <1..357
                     /locus_tag="LGJ20_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBK2388840.1"
                     /translation="WLKADINSEDSRVKERIARAGQWFGETLIADDALRASLNGHLEQ
                     AAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQFIRVNGTLVGGCIGLI
                     LYLLSQLPALFPLSNF"
     gene            473..>1563
                     /locus_tag="LGJ20_004871"
     CDS             473..>1563
                     /locus_tag="LGJ20_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HBK2388841.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETD"
BASE COUNT          387 a          376 c          435 g          365 t
ORIGIN      
        1 tggctgaaag cggatatcaa cagtgaagat tctcgtgtga aagaacgtat cgcgcgggcg
       61 ggtcaatggt ttggcgaaac gttaattgcc gatgatgcct tgcgggcgtc gttaaatggt
      121 cacctggaac aagccgcgca ccgcgtcgcg cctgagtttt ccgcattcct gacgcgccac
      181 atcagcgata cagtaaaaag ctgggatgcg cgggatatgt cgcggcaaat cgagttaaat
      241 atcggcaaag atctgcagtt tatccgtgtc aacggtacgc tggttggcgg ttgtattggg
      301 ctaattttat atttgttgtc gcagctcccg gccttgttcc ccctcagcaa tttttagaaa
      361 tccatgaaaa actaagtggc taaccgcgag agagatcaaa taaaccacac taaaagaatg
      421 gtaataatgt ggcctctttt cattatctcc cgtggtacgg ggaaggaaaa tcatgtcact
      481 tatcaccgat ctacccgcca ttttcgatca gttctctgaa gctcgccaga aaggctttct
      541 caccgtcatg gatctcaagg agcgcggcat tccgctggtt ggcacttact gcacctttat
      601 gccgcaagag atcccgatgg cagccggtgc ggttgtggtt tcgctctgtt ctacctctga
      661 tgaaaccatt gaagaagcgg agaaagatct gccgcgcaac ctctgcccgc tgattaaaag
      721 cagctacggc tttggcaaaa ccgataaatg cccctacttc tacttttcgg atctggtggt
      781 cggtgaaacc acctgcgacg gcaaaaagaa aatgtatgaa tacatggcgg agtttaagcc
      841 cgttcatgtg atgcagttgc cgaacagcgt taaggacgat gcctcgcgtg cgttatggaa
      901 agccgagatg ctgcgcttgc aaaaaacggt agaagaacgt tttgggcacg agattagcga
      961 agatgctctg cgcgatgcca ttgcgctgaa aaaccgcgaa cgtcgcgcac tggctaattt
     1021 ttatcatctt gggcagttaa atcctccggc gcttagcggc agcgacattc tgaaagtggt
     1081 ctacggcgca accttccggt tcgataaaga ggcgttgatc aatgaactgg acgcgatgac
     1141 agcccgcgtt cgtcagcagt gggaagaagg ccagcggctg gccctgcgtc cacgcatttt
     1201 aatcaccggc tgcccgattg gcggcgcagc agaaaaagtg gtgcgcgcga ttgaagagaa
     1261 tggcggctgg gttgtcggtt atgaaaactg caccggggcg aaagcgaccg agcaatgcgt
     1321 ggcagaaacg ggcgatgtct acgacgcact ggcggataaa tatctggcga ttggctgctc
     1381 ctgtgtttcg ccgaacgatc agcgcctgca aatgctcagc cagatggtgg aagaatatca
     1441 ggtcgatggc gtagttgatg tgattttgca ggcgtgccat acctacgcgg tggaatcgct
     1501 ggcgattaaa cgtcatgtgc gtcagcagca caacattcct tatatcgcta ttgaaacaga
     1561 cta
//
LOCUS       DAFATA010000253         1527 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFATA010000253 DAFATA010000000
VERSION     DAFATA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.255"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..699
                     /locus_tag="LGJ20_004872"
     CDS             <1..699
                     /locus_tag="LGJ20_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBK2388842.1"
                     /translation="CHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPA
                     PAYACGLRAYIDHEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTI
                     IRRNGFRFWGNRTPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISA
                     IRAKLDSLVTAGKLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFT
                     DEYFEPAFAVLGGA"
     gene            699..1211
                     /locus_tag="LGJ20_004873"
     CDS             699..1211
                     /locus_tag="LGJ20_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBK2388843.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHSYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            1266..>1527
                     /locus_tag="LGJ20_004874"
     CDS             1266..>1527
                     /locus_tag="LGJ20_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388844.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTI"
BASE COUNT          380 a          367 c          439 g          341 t
ORIGIN      
        1 tgtcacggct gtaacacgat ggctgaggcg attacctatc gccagaaatt caacgaacgt
       61 gaggtgatgc tcttatggcc ggacttcatc gcctacaacc cgaaaagtgg caaaaacgaa
      121 acgttccccg cgcctgccta tgcgtgcggc cttcgtgcgt acattgacca tgagcagggc
      181 tggcacaaat cactgtccaa cgttccggtt aaaaatgtgc tggggatgtc gaggcatgtg
      241 ttctggtcgt tgcaggccga agacagcgat gccaacagcc tcaacaacaa agaaatcacg
      301 accattattc gtcgcaacgg gttccgcttc tggggcaacc gcacaccgga aacgaacgcc
      361 tacatctttg aggtgtatac ccgaaccgca caggtgttgg ctgattcaat tgcggaagcg
      421 cagtttgaaa ccatcgacag tccactgacg cctgcgaacg tgaaggatgt tatcagtgcc
      481 atcagggcaa aactggattc actggttact gccgggaaac tgattggcgc ggagtgctgg
      541 tatgacgtgg tggataacag caccacggat ttacgtcagg ggcgtgtgcg tattcgctac
      601 aaatatacgc ctgttccccc gctggaagat atggagcttt accagacgtt tactgatgaa
      661 tactttgaac ccgcatttgc ggtgctggga ggtgcctgat ggctgtgcca aaacatcttc
      721 gcttttttac gctgtttgtg gatggtgaaa acgaagtggg taaggtgacg tccgtcactc
      781 tgcctaagct gacgcgcaaa accgacagct accggggtgg tggcatgatg ggtgcggtaa
      841 gtattgatct cggtctggac gactccgcgc ttgatgcgag ctttgtcatg gggggcgcag
      901 ttcgtgagct gttccttaag tatggcggca cgattgacgg cacgctgctg cgttttgcgg
      961 gtgaatacta caccgatgca gaaagcgacc tgtatgaagt cgaaatgcgc ggacgtgtga
     1021 cggaaattga tatgggggaa gccaaacagg gcgaagccac atcacactct tacgccatta
     1081 aaaacaccta ctacaagctg agcgttaacg atcgcccgct gtgggagatt gacctgctga
     1141 acttcattta ccggaaggac ggcaaggaca ttgtgcccga tcgcatccgt tccgcgcttg
     1201 ggcttggctg ataagtaata tgcaggcggc gcagtgcgtc gcctctgact gaaaggagtt
     1261 tcctgatgaa agagacgaaa aacatcgata ccgaaaacac ggtagttgct gacactgtga
     1321 aagaaaccag tgagcgtggc gtaaaactta cccaaccaat tgagcgaggc ggcgaaaaaa
     1381 tcacgtatgt ggagatcacc ggggctattg agcaggctgg atctctgcga gatttgtcgc
     1441 tgtctgatgt gctgaatctg aaagcggaat ccatgtttac gctgctgtca cgcgtgacat
     1501 caccgcgact ggatgaagtg acgatca
//
LOCUS       DAFATA010000254         1525 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFATA010000254 DAFATA010000000
VERSION     DAFATA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.256"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..873
                     /locus_tag="LGJ20_004875"
     CDS             <1..873
                     /locus_tag="LGJ20_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HBK2388845.1"
                     /translation="SSIPVTEALSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGR
                     MLQNGDTLIVSTDRYASTIQVRVEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNA
                     VQLYRPSVAQRQKEMLNLSLQKLEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTV
                     VPKGEVILNESNIDSVLLEDGDVINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIE
                     KCGGLTQKSGNARIIVIRQNGAAVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTI
                     LYQLAVGAKVILSL"
     gene            883..>1525
                     /gene="kdsB"
                     /locus_tag="LGJ20_004876"
     CDS             883..>1525
                     /gene="kdsB"
                     /locus_tag="LGJ20_004876"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBK2388846.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIR"
BASE COUNT          387 a          376 c          428 g          334 t
ORIGIN      
        1 agcagcattc ccgtaacgga agctttgagc tgggcgcgcc ctaagcctgg cgcgactcac
       61 attacgatta tgcgtaaaca ggggctacaa aaacgcagcg aatactatcc gatcagttct
      121 gcgccaggcc gtatgttgca aaatggcgat accttaatcg tgagcactga ccgctatgcc
      181 agcaccattc aggtgcgggt tgaaggcgca cactccggtg aacatgccat ggtactgcct
      241 tatggttcca ctatgcgtgc ggttctggaa aaagtccgcc cgaacagcat gtcgcagatg
      301 aacgcggttc agctttatcg cccatcagtg gctcagcgtc agaaagagat gctgaatctc
      361 tcgctgcaaa agctggagga agcctcactt tctgcccagt catccaccaa agaagaagcc
      421 agcctgcgaa tgcaggaagc acaactgatc agccgctttg tggcgaaagc acgcaccgtg
      481 gttccgaaag gtgaagtgat cctcaacgaa tccaatattg attctgttct gcttgaagat
      541 ggcgacgtca tcaatattcc ggagaaaaca tcgctggtta tggttcatgg cgaagtgctg
      601 ttcccgaacg cggtgagctg gcagaagggt atgaccaccg aggattacat cgagaaatgt
      661 ggtggcctga cgcaaaaatc gggtaacgcc agaattatcg tcattcgtca gaacggtgct
      721 gccgtcaacg cggaagacgt ggattcactc aagccgggcg atgagattat ggttctaccg
      781 aaatatgaat cgaaaaatat tgaagttacc cgtggtattt ccaccatcct ctatcagctg
      841 gcggtgggtg caaaagtgat tctgtctttg taaggagttg aaatgagcaa agctgttatt
      901 gtcatcccgg ctcgttatgg ctcatcgcgt ctgccgggta agccgctact cgatattgtc
      961 ggcaaaccga tgatccagca tgtttacgag cgtgcgttac aggtggcggg cgtcgcagaa
     1021 gtttgggtgg caacagacga tcagcgtgtt gaaaaggccg tgcaggcatt tggcgggaaa
     1081 gccattatga cgcgcaacga tcatgaatcc ggcaccgatc ggctggtcga ggtgatgcat
     1141 aaagtcgagg cagatatcta cattaacctg cagggcgacg aaccaatgat tcgcccgcgg
     1201 gatgtagaaa cgctgctaca aggaatgcgt gatgatcctg cgttgccagt ggcaacgcta
     1261 tgccacgcga tttctgccga agaagcggct gagccaagca cggtaaaagt ggtggtaaat
     1321 acccgccagg atgcgcttta tttcagccgt tcgcctattc cgtatccgcg taatgctgaa
     1381 aaagcgcgct acctgaaaca cgttggtatt tacgcttatc gtcgcgatgt gctgcaaaac
     1441 tacagccagt taccggagtc catgccggag caggcggaat cactggagca gctgcggttg
     1501 atgagcgcgg ggatcaacat ccgca
//
LOCUS       DAFATA010000255         1497 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFATA010000255 DAFATA010000000
VERSION     DAFATA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.257"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(1..593)
                     /locus_tag="LGJ20_004877"
                     /pseudo
     CDS             complement(1..593)
                     /locus_tag="LGJ20_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(624..950)
                     /locus_tag="LGJ20_004878"
     CDS             complement(624..950)
                     /locus_tag="LGJ20_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388847.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(950..1369)
                     /locus_tag="LGJ20_004879"
     CDS             complement(950..1369)
                     /locus_tag="LGJ20_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388848.1"
                     /translation="MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKG
                     DGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLG
                     LFKNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          384 a          385 c          317 g          411 t
ORIGIN      
        1 aacaccacac tgccaccgtc ttcgccgccg gactgctgaa gggtatccgc caggctgcgg
       61 cgctgcgttt tcgcctgctc accggtggcg gtttcaaccc tgatgagtgg cgtgaagctc
      121 cccggctgat aaatcgtctg gatgcgggtt ctgtcgttct gtattgtggt caggcggtcg
      181 ccgtcccagc cgtaccaggt cacttgcggt ttccgtgaca gcgacatcca gcccgtcagg
      241 tcccgttcac gtcgccacac ccgttttgcc accctgcggc ccagcgggtc gtaaagatag
      301 cggctttcga ccagcggctc tgcatattgt gtccgcgtgt agtgcaccag ccggtgctga
      361 ctgtcgtaat ggtaccggtg ggtgcgctca tcatccgtgc ggataacccc ttccgggatg
      421 aggtcggttt tctccgtcag cctgccgtga cggtcatacc ggtaaagata gtgcgcgtca
      481 cgggcgatac ggttatccgg ccacatgctg agggcgctgt ccgggtgcag ctccgggtcg
      541 ggcaggcggt tacctgccgg gtctgtggca tacgggatat caatgcccag gttacatata
      601 ataataagag caattaactt aatttatttg tttataatgc gcttacttaa tacaaaccat
      661 tgaatatacc ctcctgacag aaaataaagc catattatta tcgccataat atacttacta
      721 tcaatcctta cgccaataat gtctgataca gtccctataa atattagagc aataaagaat
      781 actatgcctg ttggaaaaca tattaccatc attccataag acatgagact aattaactcg
      841 ctcacatcac gagtggagtc taatagtgaa agtctatgca tggaaaagaa tagtattaaa
      901 agcgataaag ccagccatat agctctgaat aaaaaaataa tatatttcat tacatcactc
      961 cggtgaatac aaatctaata aaacgtccaa tgttatttcc ggagcacttc atcttacatg
     1021 tttctaaaga atcattagac cagtcttggc agtttttaaa tagccctaaa tttataggtg
     1081 tgaatatatc aggcaataca gaataacgcg gccttacctc tttaaacttc ccttttaaac
     1141 agttctccac acaactatta tcggtttcaa cgaggtcaca acggccagca gcttcttttg
     1201 tatcattact tgctttacct ggcccccata gaccatctcc ttttgattct ccccgttggt
     1261 cttggccacc acaagtatag tcccctttac catctgggac acaaagatat tgatgataga
     1321 acggattccc ccatatatct ggaccgcttc tttcaccagt tccgcccatt gaatgcagtg
     1381 gcttaatgca ttttagtgtt tctaatccta atggatctat atttgagatc ggattcaacg
     1441 gatactgata aaaattccat ccccccttca gcccaatcgg atcctgagtg atatacc
//
LOCUS       DAFATA010000256         1467 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFATA010000256 DAFATA010000000
VERSION     DAFATA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.258"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..535)
                     /locus_tag="LGJ20_004880"
     CDS             complement(<1..535)
                     /locus_tag="LGJ20_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HBK2388849.1"
                     /translation="MSKLSSEMKALAKKAGGSFKTVNDRIHITKRFSEHLRALNIQIQ
                     RVEQIKVRHIECYIEERLEQDIGLRTLQNDMAALQAVLRQAGRRQVVEHPRLTNKALG
                     VSGASRNGTRRAITPEHYQQVMEKARTEDAGLAAALEIARLMGLRSQEAVQSSQSLKT
                     WLKAIERGENRLKVVFGT"
     gene            759..908
                     /locus_tag="LGJ20_004881"
     CDS             759..908
                     /locus_tag="LGJ20_004881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388850.1"
                     /translation="MSDGGNMAQAGTGAACGGMLCSVTLPCRWRDQSDASSGAEGKRC
                     VSGAG"
BASE COUNT          282 a          426 c          403 g          356 t
ORIGIN      
        1 tggtgccaaa caccactttc agccggtttt ccccgcgctc aatggctttc agccacgttt
       61 tcagcgactg tgaactctgt accgcctcct gcgaacgcag ccccatcagc cgcgcaatct
      121 ccagcgctgc tgccagccct gcgtcctcag tgcgggcttt ctccatcacc tgctggtaat
      181 gttccggcgt gatggcgcgg cgcgtgccgt tgcgcgaagc gcctgacacc cctaatgcct
      241 tattggtcag acgcggatgc tccaccacct gcctgcgtcc agcctgtctc agcacggcct
      301 gcagtgccgc catatcgttt tgcagcgttc gtaaaccaat atcctgctcc agccgttcct
      361 caatgtagca ttcgatatgg cgaaccttga tctgctccac ccgctggatc tggatattca
      421 gcgcccgtaa atgttcactg aaccgttttg taatatggat acggtcattc accgttttga
      481 agctgccgcc cgctttcttc gccagcgcct tcatttcact gcttaattta ctcatgaatc
      541 ccctgcaaca taaacaaaca gtctccggcg tgtgctgttt ggctgcgcca gaacgatacc
      601 ttcacgccgg agtggctggt tgttgaggtt tagggagttt caggctgtac gcggtacaga
      661 ctgcctgacg aagcaggggt atctccgggg cttctagccc cccctcggta tcgatctttt
      721 tctcctctta ctctgagctg acagcggccc caaagggcat gtcagacggc ggaaatatgg
      781 cgcaggccgg gactggcgcg gcttgcggcg gtatgttgtg cagcgtcact ttgccctgtc
      841 gctggcgcga ccagagtgac gcctcgtctg gtgcagaggg taaacgttgc gtttctggtg
      901 cagggtgacg gtcacgcggc agcaaagcct gctgtctttg caggccgcag cccgtcaatg
      961 taaggcgctg gttgtcagca gaagataaaa cccggcaaag ccagagtgcc agcccgtaaa
     1021 gccgggtatc tggtgcagtg atggtgtata tgtacgtcag tctcagatgc cggtattttt
     1081 gcatccccgg cgttttcaga gatcaacgcc ggtaaaggat gtcatgctgg caatgccagg
     1141 cgcttctttc agacgcgaag cgacaaaggg actgcccgga ggcagtttgt tgcaggcggg
     1201 agactgcgcc attatttctg gtgcaggaag gaaaaactct ttccgggttt gaaagtatgt
     1261 ttgcttacct ggtgacggaa aaactgccgc gcttcgcttg tcttctcccg gcgcgctgcc
     1321 agcaaaaaca ggcagagcgc cgggagagtc tggttttact gtaacagtgt tcctgccggt
     1381 tcagttatca ggactaacac tttcacatcg gtcctccggt atcggtgtca attccccaac
     1441 aggtgcaact ctgacggcaa cgctaac
//
LOCUS       DAFATA010000257         1445 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFATA010000257 DAFATA010000000
VERSION     DAFATA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.259"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            32..1144
                     /locus_tag="LGJ20_004882"
     CDS             32..1144
                     /locus_tag="LGJ20_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HBK2388851.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT          355 a          352 c          395 g          343 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aaacgctgga agcagcgggc catgtgctat tgctaacatc
      901 attaccggaa gatgaatatt cagcagagca agtggctgat tgttaccgtc tgcgatggca
      961 aattgaactg gcttttaagc ggctcaaaag tttgctgcac ctggatgctt tgcgtgcaaa
     1021 ggaacctgaa ctcgcgaaag cgtggatatt tgctaatcta ctcgccgcat ttttaattga
     1081 cgacataatc cagccatcgc tggatttccc ccccagaagt gccggatccg aaaagaagaa
     1141 ctaactcgtt gtggagaata acaaaaatgg tcatctggag cttacaggtg gccattcgtg
     1201 ggacagtatc cctgacagcc tacaaaacgc aattgaagaa cgcgaggcat cgtcttaacg
     1261 aggcaccgag gcgtcgcatt cttcagatgg ttcaaccctt aagttagcgc ttatgggagc
     1321 aatccccgca tatccggttg tcaggtcagg atggtaaggc acctgcttta cactttcgcc
     1381 cgtggtcagt gatggctgcg ggcgaatcgt accagatgtt gtcaattaat cgtgttggca
     1441 cagcg
//
LOCUS       DAFATA010000258         1441 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFATA010000258 DAFATA010000000
VERSION     DAFATA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.260"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..425)
                     /locus_tag="LGJ20_004883"
     CDS             complement(<1..425)
                     /locus_tag="LGJ20_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBK2388852.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGEAPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSR"
     gene            complement(418..1326)
                     /locus_tag="LGJ20_004884"
     CDS             complement(418..1326)
                     /locus_tag="LGJ20_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBK2388853.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLTERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVKRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(1313..>1441)
                     /locus_tag="LGJ20_004885"
     CDS             complement(1313..>1441)
                     /locus_tag="LGJ20_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBK2388854.1"
                     /translation="TLDSITINSNFDGSMVVELTGRRNNGVPVSLSVSTGAENGSD"
BASE COUNT          301 a          457 c          376 g          307 t
ORIGIN      
        1 cggctacatg gtttggcatc gcttatcagt cgctcaagtt ccagataggt atcttcagtg
       61 atgccctggt cctgcacgcc gatatccagg cgaaacgtgc ccggtgcctc tccggtctgc
      121 caccactcaa taatgcggat cagaaagccg aacggctcca ccacccgccg cacggcactg
      181 gtggtccctt tatgctgatg aatataaaaa gcatccttca ccacctggcg cttgacgctt
      241 tctgtccagc cctcgtccca gcgatccaca gagaacgccc aggcgagata aggcaggaaa
      301 ctgaccggac aggttgccgg attccacaag tcacgcagcg gcacctgcag atcagaaatc
      361 ccgctgcagg tttgcgccag tcggcgctcc agtggtgttg aacccggtgg cagcagacta
      421 ttcatccgtt cctccgttgg ttacgctcca ctgcgtacat gatgccgcct gtgttttgtt
      481 caggaccaca tccgccagcg gcgaagccag ctccacacgc tgcacaccct caacatgcag
      541 ggcggcaaag atggcgctac ggcgaatatc ccggccaaga cgcgtctgac tggcgatata
      601 cttctgcaga ctggcttttg ccgctgccat taccggctct gcttccggtc ccggatagag
      661 aaaaatggtg gcttccacgc ggtacgggat gatttctgcg ctgcgaaccg tcagacggtc
      721 agccaccggg cggacgttct cactgttcag agctttttcc accacgtcca gcaggtcttt
      781 ttctgcagtt ccatcgcctt cgcggctaag aacagtcagc accacctctg caggtgccgg
      841 gctggttgca ctggcatccg ccacccgacc gtcggcgctt cgtgcatgaa attcataagc
      901 tgcagttggc cccgcaactg aaagcccttc aaaggctgcg ggcacacgca ggcgtaacgc
      961 ttcatcgctt tccatcacag ccgcaacggg cggcacagcg tcattatcag caggcgtcac
     1021 cgtcaggcgt ttcacgttgt agttggcagc gagctggtca agatcgccgc ccatcgcgta
     1081 agccaccatc acagcctgcg cggcttcgtt aatgcgctgg cgcagaagca actcacggta
     1141 agcattctcc tgcaacaatt tggtgacggg ttcagattcc agttccagcg tgcggatcac
     1201 tgcttcctgc tcatctttcg gatgaagcgc cacaaattct gccttgcgtt cggtaagcag
     1261 cgtctcaaag tccggcacat ccacaatctg cggcgcaggc aactgcgaaa ggtcaatcac
     1321 tgccattctc tgctcctgtt gatacggaaa gggacacagg cacaccgtta ttccgccgcc
     1381 cggtcagctc caccaccata gaaccgtcaa agttgctgtt gatggtgatg gaatccagcg
     1441 t
//
LOCUS       DAFATA010000259         1432 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFATA010000259 DAFATA010000000
VERSION     DAFATA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.261"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            23..1405
                     /gene="dosC"
                     /locus_tag="LGJ20_004886"
     CDS             23..1405
                     /gene="dosC"
                     /locus_tag="LGJ20_004886"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBK2388855.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
BASE COUNT          407 a          301 c          337 g          387 t
ORIGIN      
        1 cagccttata agggtgtgaa ttatggagat gtattttaaa agaatgaaag atgagtggac
       61 cggacttgtc gaacaggcag atcctctcat tcgtgctaaa gccgcagaaa ttgcccttgc
      121 gcatgctcat tatctgagta tcgagtttta tcgaattgtc cgcatcgacc cgcatgccga
      181 agaattcttg agtaatgaac aagttgagcg gcagttgaaa agtgcgatgg aacgctggat
      241 tattaacgtg ctttctgccc aggttgacga tgtcgaaagg ctaatacaaa tccagcatac
      301 cgtcgcggaa gtgcatgccc gcattggaat tccggtagaa attgtcgaga tggggtttcg
      361 ggtgctgaaa aaaatcctct atccggtaat cttctcttcg gattattctg ccgcagaaaa
      421 acttcaggtc taccatttct cgattaacag tattgatatc gctatggaag tgatgacccg
      481 cgcgtttacc tttagtgaca gtagtgcctc aaaggaagat gaaaactatc gtatcttctc
      541 gttactggaa aacgccgaag aagaaaaaga acggcaaata gcctcaatac tttcatggga
      601 aatagatatt atctataaaa tcctgctgga ttctgattta ggcagtagtt tgcctttaag
      661 ccaggctgat tttggcctgt ggtttaacca taaaggtcga cattatttta gcggcattgc
      721 cgaagtaggc catatctccc gtctgattca ggatttcgac ggtattttca atcaaaccat
      781 gcgtaacacc aggaatttga ataacagaag tttgcgggtg aagtttctat tacagataag
      841 aaataccgta tcgcaaatta ttaccttgct gcgtgaattg tttgaagaag tatcgcgcca
      901 cgaagtcggt atggatgtac tgacgaaatt acttaaccgc cgtttcctgc cgactatctt
      961 caaacgcgaa attgcccatg ccaaccggac cggtacaccg ctgtcagtgc tgattattga
     1021 cgttgataaa ttcaaagaga tcaacgatac gtggggccat aacactggcg atgaaattct
     1081 gcgtaaagtc tctcaggcct tttatgacaa cgtccgcagt agtgattatg ttttccgcta
     1141 cgggggcgat gaatttatca ttgttttgac tgaagcttct gaaaacgaaa cgttacgtac
     1201 cgcagaacgt attcgcagtc gggtggagaa aaccaaactg aaagccgcaa acggcgagga
     1261 tattgccctc tcactttcca tcggtgccgc catgtttaat ggtcatcctg actatgagcg
     1321 cctcattcaa atagccgatg aagctctgta tatcgccaaa agacgaggta gaaaccgtgt
     1381 tgaactctgg aaagccagtc tttagatgcg ccaggatgca gaggtaatca tg
//
LOCUS       DAFATA010000260         1402 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFATA010000260 DAFATA010000000
VERSION     DAFATA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.262"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..376
                     /locus_tag="LGJ20_004887"
     CDS             <1..376
                     /locus_tag="LGJ20_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK2388856.1"
                     /translation="EIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYN
                     VVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLE
                     GLKKQFDPNGIMNTGTIYPIEK"
     gene            398..>1402
                     /locus_tag="LGJ20_004888"
     CDS             398..>1402
                     /locus_tag="LGJ20_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388857.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     K"
BASE COUNT          294 a          367 c          364 g          377 t
ORIGIN      
        1 cgaaatctac gaaagcgtta ttaaccgtat tcgtactgag ttcccgcacg ccgacgacat
       61 caccatgctg ggcggtcatt cctctcatag ctatcagaac ggcaccaaca tgtacttcgt
      121 ctacgattac aacgtcgttg actgtaagcc ggaagaggaa atcgacaagt accacaatcc
      181 gctcaacaag atcatctgtg aagaaaccat tcgcctcggc ggttcgatgg tgcaccacca
      241 cggtatcggt aaacatcgcg ttcactggag caaactggaa cacggcagcg cgtgggcgtt
      301 gctggaaggg ctgaaaaagc agttcgatcc taatggcatt atgaatacgg gtactatcta
      361 tccgattgaa aaataatgtg tcaggcagcg ttccgcgatg acggggcgtt gccttttcgg
      421 cttctcaggc gagaagccgt tcttattacc ggacaatgaa ggggtaaaga tgaacacttc
      481 accggtgcga atggatgatt taccgcttaa ccgttttcac tgccgcattg ctgcgctcac
      541 tttcggcgca cacctgaccg acggttatgt tctcggcgtc attggttacg ccattattca
      601 gcttacgccc gccatgcaac tgacgccgtt tatggcggga atgatcggcg gctcggcgct
      661 ccttggtttg ttccttggca gcctggttct tgggtggatc tccgaccata ttggtcggca
      721 aaaaatcttc accttcagct ttttgctgat tacgcttgct tcgtttttac aattttttgc
      781 caccacgcca gagcatctta ttggactgcg cattttgatt ggcattggtc tgggaggcga
      841 ttattcagta ggtcacacct tgctggctga attttccccg cgccgccatc gcggtatttt
      901 gctgggcgca ttcagcgtgg tgtggaccgt aggctatgtg ctggcaagta ttgccggaca
      961 tcactttatt tccgaaaacc cggaggcctg gcgctggcta ctggcatcgg cagctctgcc
     1021 cgcgttgttg attacgttat tacgctgggg aacgccagaa tcaccacgct ggctactgcg
     1081 ccaggggcgt tttgcagaag ctcacgctat cgtgcatcgc tattttggtc cccatgtttt
     1141 actgggcgat gaagtggtaa cggcgaccca taaacacatc aaaaccttgt tctcttcgcg
     1201 ttactggcgg cgcacggcgt ttaacagcgt cttctttgtc tgcctcgtaa tcccatggtt
     1261 tgtgatttat acctggctgc caactatcgc ccagactatt ggtctggaag atgcgctgac
     1321 tgccagcctg atgcttaatg cgttgttaat tgtgggcgcg ctgctgggat tagttctgac
     1381 gcacctgctg gcacatcgca aa
//
LOCUS       DAFATA010000261         1387 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFATA010000261 DAFATA010000000
VERSION     DAFATA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.263"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..140
                     /locus_tag="LGJ20_004889"
     CDS             <1..140
                     /locus_tag="LGJ20_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388858.1"
                     /translation="MAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKA
                     H"
     gene            complement(183..1037)
                     /gene="ampE"
                     /locus_tag="LGJ20_004890"
     CDS             complement(183..1037)
                     /gene="ampE"
                     /locus_tag="LGJ20_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBK2388859.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(1034..>1387)
                     /gene="ampD"
                     /locus_tag="LGJ20_004891"
     CDS             complement(1034..>1387)
                     /gene="ampD"
                     /locus_tag="LGJ20_004891"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBK2388860.1"
                     /translation="IAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQYQGRERCND
                     FSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPDRKTDPGPA
                     FDWARFRVLVSKETT"
BASE COUNT          394 a          397 c          320 g          276 t
ORIGIN      
        1 ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc
       61 tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca
      121 aagccgtaaa agcgcattaa tctctctacg ccctcacccg tacagggtga gggcaataat
      181 ctttacacca acgccccgta aatcgtcagt agtgcaatca ccaccacgac cacgaacgag
      241 gttttcttcg ccattgaaac cgctgccttc ggcgtctcca ccttatcgac atgcggttca
      301 cgcgccagag agaactgcgc cagacgcgtt aacacctgat actgcgaagt atggaaatca
      361 cccagcgaag caaaccaggc tggtaacgct ttctcaccat gaccgatcaa ggcatatacc
      421 acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac
      481 tgtaaacgat gatgcggcgt ctgatatcgc gccagccagt attgccatgc acgcaaaaac
      541 gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt
      601 gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca
      661 cgttcgtcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca
      721 cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact
      781 ttacctgcgc caatacacag caaaccaatc agcagccaca ccagtagcgt gggaacgttg
      841 aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc
      901 atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga
      961 tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag
     1021 gttgtaaata gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt gcccaatcaa
     1081 atgcaggacc gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgtttt
     1141 tagcgatatc cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact
     1201 gcgcatcggt atacgccagc gtatcggtgc cttcaagctc aatcccaata gaaaaatcat
     1261 tgcagcgttc gcgcccctga tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag
     1321 gaacatactg gactatttca ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat
     1381 gggcgat
//
LOCUS       DAFATA010000262         1381 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFATA010000262 DAFATA010000000
VERSION     DAFATA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.264"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1295)
                     /locus_tag="LGJ20_004892"
     CDS             complement(<1..1295)
                     /locus_tag="LGJ20_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2388861.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPI
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQVHERELILNDSGGRSIHFEPLFPGEVSYSRSESFWLARGGVAEQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGP
                     RSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGS"
BASE COUNT          251 a          507 c          366 g          257 t
ORIGIN      
        1 atgctcccgt ccgcatgttc cttcttcacc acccgcttca ggccaccctc gccttccgtg
       61 tacagcacct cccgccggtt caggctgtcc gtgatgatta cccggctctc tccgtactca
      121 aagcggtagt ccagcccctc cgggttgacc tgctccgtca cccgaccggt atcatcatac
      181 cggtagcagc tctccggcct ccccgcataa tggtgcgcca ccatccgccc ggcgtgctcc
      241 gcatcataag taaacccgcg cacctgcgtt ccgctgcggt catacaccgc ccgcagttca
      301 ccaccggccg tgtacgtgta gcgcgccagc ggcgcagtcg gctgttcatc cgggtatgcc
      361 gggtcgtgtg tcagccatac cgcctccagc cggataccgt tatccgtgcc gtatcctgta
      421 ccggcgggca gcgtgtcggg gaaaaccaga gaagaggaag cagaacgggg accggcaggg
      481 gaagataaag acgtggcgcg ctgtttacgg aatacttccg cccgctgtgc ctgtgtggtc
      541 agcaccaggt gaaaacggcg gcccgcgccg tccgtcaccc ccgtcaccgc gcccgccaca
      601 tcaccctccg ccgcccggtg aaaggccagc gtccgcccga agccgtccac cacgcccgtc
      661 agcacccggt atgcgggcgg ctccggcggc agcacctcgt ccgcccccgg cacccgctcc
      721 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      781 ctcaggcgaa catcttccgg cagcacctgc cagagcgcgc ttagcggctg cgaactgtgc
      841 tgctccgcca ccccgccccg cgccagccag aacgactcgc tgcggctgta gcttacctcg
      901 ccgggaaaca gcggctcaaa gtgaatgctc ctgccgccgc tgtcgttgag tatcagttcc
      961 cgctcatgaa cctgtaagcg gatatcgaag ggtgctttcc agccgggacc gaacaccccc
     1021 accggcgcgg gcgtcctggt ccggtaactg ctgtaggcgc gggaaagaat aaacggcaac
     1081 gggccgggca gcgcaaggtc cgtctcgccc ggcagcacct ttgctcccag cagcgggttc
     1141 accgggttac ccttctgctc ctctatcggg ctgttgggac ataccgaaca ggccactccc
     1201 gtcggcgcgc ctatcagcac ccctgctgaa ccctgcacaa tatccagtcc cttgcgggtc
     1261 atgtcgccct gccgcgctgc cggttttccg ctcattctca ctcccttata tttataccac
     1321 taacaataaa aaagccctca acaaaacatc accaacatat attaacgata tatttttatt
     1381 t
//
LOCUS       DAFATA010000263         1330 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFATA010000263 DAFATA010000000
VERSION     DAFATA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.266"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..407
                     /locus_tag="LGJ20_004893"
     CDS             <1..407
                     /locus_tag="LGJ20_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388862.1"
                     /translation="VLMYVMLWRIDAGDYAGALEIGRHALRHGWVMPLGNRNVQTVLA
                     EEMADAAQSAMLAATGFDADLLLQTLELTDGLDMPDQSRARLHKAIGAVLSESNPASA
                     LNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            503..967
                     /locus_tag="LGJ20_004894"
     CDS             503..967
                     /locus_tag="LGJ20_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBK2388863.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYVSLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAESSGDLWRDARWAISRVQDAPHCTVELI"
     gene            967..1170
                     /locus_tag="LGJ20_004895"
     CDS             967..1170
                     /locus_tag="LGJ20_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HBK2388864.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKTNPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            1174..>1330
                     /locus_tag="LGJ20_004896"
     CDS             1174..>1330
                     /locus_tag="LGJ20_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388865.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKA"
BASE COUNT          288 a          360 c          421 g          261 t
ORIGIN      
        1 acgtgctgat gtacgtgatg ctgtggcgca ttgatgccgg agattatgcc ggggcgctgg
       61 agatcgggcg tcatgccctg cgtcatggct gggtgatgcc gctgggtaac cgcaatgtgc
      121 agaccgtgct ggcagaggaa atggcagacg cggcgcagag cgcaatgctt gccgccaccg
      181 gctttgatgc cgatctgttg ttgcagacgc tggagctgac agacggtctg gatatgccgg
      241 accagtcacg agcgcgtctg cataaagcga ttggcgctgt cctgagtgaa agcaatccgg
      301 cttctgccct taaccatctc aaccatgcgt tacagctcga tccccgctgt ggcgtgaaaa
      361 aagacaaaca gcagctggag cgcagactgc gcaatgacag ccgctgacag aacgtgcccc
      421 cgcgcacggg cggcacgggg tggcgaaagg catagccaca tcaaaatccc gtccaccgcc
      481 ctctatttca ggagaaagca gcatgaagtt tgttgcgcca gaacaggcac cggaacaggc
      541 ggaaatcatc agaaatacgc cgttctggcc tgatgtggac ctgtcggagt ttcgcagcgt
      601 gatgcgcact gacggcacgg tgacgcagcc gcgtttaaag caggttgccc tgtcggcaat
      661 ttcggaggtc aacgcagagc tgtatgagtt tcgcagacgt cagcagatgc tgggatatgt
      721 gtcgctggct gaggttccgg cggaacagct ggacggcaaa agtgagcgca ttcagcacta
      781 tttcaacgcg gtttactgct gggcacgcgc catgctcaac gaacgatacc aggactatga
      841 cgccacggca tccggtgtga agcgaggcga ggaactggcg gaatccagcg gtgatttgtg
      901 gcgtgacgcc cgctgggcca tcagccgggt gcaggatgcg ccgcactgca cagtggagct
      961 tatctgatga aagtgcgtgc gcatcagtat gacacggtgg acgcgctttg ctggcgtcat
     1021 tacgggcgca cgcagggtgt cacggagcag gtactgaaga caaatccggg gcttgccgaa
     1081 tacggcccct ttttacctca cgggctgcag gtggagctgc cggacattcc gacaaccacc
     1141 accgtgcaga ccgtccagct atgggactga attatgacgc ttgagcgaat cagcgccttt
     1201 atcacgtatt gcatcgccgt cgtgctggcc tggctgggcg atttgtccat caaggatgcc
     1261 tcaacgctgg gcggcctgat gatcggtgtg ctgatgctgg ctatcaactg gtactacaaa
     1321 cacaaagcct
//
LOCUS       DAFATA010000264         1315 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFATA010000264 DAFATA010000000
VERSION     DAFATA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.267"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..877)
                     /locus_tag="LGJ20_004897"
     CDS             complement(<1..877)
                     /locus_tag="LGJ20_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBK2388866.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGT"
     gene            1000..1257
                     /locus_tag="LGJ20_004898"
     CDS             1000..1257
                     /locus_tag="LGJ20_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388867.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
BASE COUNT          366 a          298 c          314 g          337 t
ORIGIN      
        1 aggtgcctaa ctgaatcaca gcgaggcgga aaggacgagc ctcttttgcc cgctgcggtg
       61 cgacttccgc gatcagctca cgcaggtaac tttcttcaaa acagtgcgca tcaatgccac
      121 cgataaagcc atacgggtta cgtgccgttt ccagataaac cggtgttgca ccagcctgta
      181 gcaacgctcc gtggtggtta gatttgtggt tattgcgatc aaacagcacc agatcacccg
      241 gtgttagcag ggcgtttaaa accactttgt tagaagatga agtgccattt aaaacgaagt
      301 aggttttatc ggcattaaac acttttgccg catgttgctg tgcaatgcat ggcgcgcctt
      361 cgtgaatcag cagatcgccc atcgctacgt cggcgttgca caagtcggca cggaacagcg
      421 cctcaccaaa gtattccaca aactgattcc ccgccggatg gcgacggaaa aattcgccgc
      481 cctgatgacc agggcaatca aacgcgctgt taccttgatt gacataatcg accagtgcgc
      541 ggaagaaagg tgggcgcagt tgagtttcat aatggctggc agcggtttct aactggcgac
      601 cataaaattc tcgtcgcgat tcgcaattct caaagacacc cgaaatacgg ggcaaatact
      661 ctgcgggtac acgttcttga ttttcagtag caataaatac cgggatgcca taacccgtgg
      721 catcgatttc atcgagtttt ccgcatgtaa catcgttgag cgataaaact attgccgcca
      781 catcgatatt acgactttca ttaatgtaga tacattctcg ctgcgtggta aaacagtccg
      841 ggcaagaatc actaaccgca atttttaatt ttgacatttt tcatctcttt attttaggta
      901 atagcaggtc ctcaatttct cgaatgagaa atcgatcgtg ccggaaaaaa gcaaagttac
      961 cccgctgata aataaatcag gtagattgct tttttcagaa tgcagaaacc cttaccagtc
     1021 aggtggctga taatacaagg gttaacagag aatgagcact gcggaataca ggcatatgcc
     1081 tgtaatacag agagtcagga agacctgtac gctgaagtgc ccttagtcgg tagactaacg
     1141 ggcattgaag aagtgaaagt cgaagctatt gcgatgagca aacttcataa tatgagttgt
     1201 ccgccttata tggggcatag tgcggctgtt attttccatt taatttacct ttcctgatgt
     1261 gaatgaaggt gaagatattc tatccaggaa atacggttga actgtgaagg tgatc
//
LOCUS       DAFATA010000265         1287 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFATA010000265 DAFATA010000000
VERSION     DAFATA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.268"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(87..1139)
                     /locus_tag="LGJ20_004899"
     CDS             complement(87..1139)
                     /locus_tag="LGJ20_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK2388868.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPNGDKVAAAVGITP"
BASE COUNT          319 a          295 c          304 g          369 t
ORIGIN      
        1 caaaaaaccc atttatgtaa acgggttaat gaaaacaata agttagagta aaaacaataa
       61 gttggattgg tggtcagtgg tggttattac ggggtaatgc caaccgctgc cgccactttg
      121 tcgccatttg gcagcgttgc cagaggattg aaacggagcg ccgtttccag atgatccggt
      181 gccagatgtg cgtaacgcat agtcattttt atatcgtggt gtccaagaat tttttgtaag
      241 gccagaatgt ttccacccga catcatgaag tgcgccgcaa acgtatggcg cagaacgtgt
      301 gtcagttgac cgcgagggag cacgatagac gttttttcca tcacggataa aaattgaaaa
      361 tagcagtctg tgaagaaatt gaacccatca agcgccatga tctcttcgta aagctcttta
      421 ctgataggga tgcttctgtt tttcttcccc ttcgttctga caaaggtaat tcggtatttg
      481 gtcacctgtg agcgggtaag atttacggct tcacgccagc gtgcgcctgt gcttaagcat
      541 atcttaacta ccagtgccag aattgggtcc tgacgtttgc aatcagccag caattcaaca
      601 atctgctcat gggtaagcca tgccatctct ttttctgcga tggtgaattt tcgcatgttc
      661 tccagtgggt tcggatacga ccattcgccc agacgggata gttcgctaaa aacactactt
      721 agatagcttt gctccaggtt aatggtgacc gggcttgctc ctttcttcca tttctcgctg
      781 aagtagatct cgcctgtcag gcgtttatct cgatagtggg caaacatttt agaggtgaga
      841 tcggttgcaa gaggattgcc cagagcatca accatcaaca gcaatttgtc atagacatgc
      901 tgcccagcgg ttagagattt accatgtagt ttgaaccata gctcaaccac gtctttcaat
      961 gtccgacgat ccactgattc gcccagccag ggctttgctt cggtttcttc catcgtgtga
     1021 cgctcaaaag ccagagcttc gcctttggtg gcgaattgtt tacgcacacg gcgtccacta
     1081 cgtccggcgg ggtaacattc gcaaagccat tttcctgtgg tgagttttcg tactgccata
     1141 aaaaatgccc tccaatagag agcattttta ctgtatgtat aaccagtgtc aatgtatgaa
     1201 atcctgcgac catacatctc actgaagcca taatgaagtt ggctattttt tgctatgtga
     1261 gtatgtgact tttgcggtta gcctgcg
//
LOCUS       DAFATA010000266         1285 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFATA010000266 DAFATA010000000
VERSION     DAFATA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.269"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..740
                     /locus_tag="LGJ20_004900"
     CDS             <1..740
                     /locus_tag="LGJ20_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBK2388869.1"
                     /translation="GSSAGGEQPKFTCYVQTPSGNKHVLVKFTVPQQTAVSQRWGDLL
                     IAESIAAQILCDGGIHAIESTVLVTSNRQVFLEAERFDCKGNDGRLPIVSLEAVQSEF
                     ISSPGSWPEAMRRLCEQQFVTHQSVAQTEVIWAFGRLIANSDMHAGNLSFYLSEPPFA
                     LTPVYDMLPMAYAPNSAGMLRDAAIEVKFDLNVSKSAWLTAIPLAQQFWQTVAGDPRI
                     SEAFRHIAQEMPEKIRQIEGKVARMGG"
     gene            complement(741..>1285)
                     /gene="lplA"
                     /locus_tag="LGJ20_004901"
     CDS             complement(741..>1285)
                     /gene="lplA"
                     /locus_tag="LGJ20_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBK2388870.1"
                     /translation="LSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCE
                     AISETFFAHYGERTEAEIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERF
                     TWGGVELHFDVEKGHITRTQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVD
                     FPEQEKELRELSAWIAGAVR"
BASE COUNT          284 a          333 c          366 g          302 t
ORIGIN      
        1 tgggttcttc tgcgggcggc gagcagccaa aatttacctg ctatgtacaa acgccgtcag
       61 gcaataaaca tgtgttggtg aaattcaccg tgccacagca aaccgcggtc agccaacgtt
      121 ggggtgacct gctaattgct gaatctattg ccgcgcaaat cctgtgcgac ggtgggatcc
      181 acgccatcga gtcaacggtg cttgtaacaa gtaacaggca ggtattcctt gaagcggaac
      241 gctttgactg taaaggtaac gatggtcgct tgcccattgt gtcgctggag gcggtgcaga
      301 gtgagtttat ctcttctccg ggatcgtggc cggaggcaat gcgccgtttg tgtgagcaac
      361 aatttgtcac tcaccagagc gtggcgcaaa cagaagtgat ctgggcgttt gggcgactta
      421 tcgccaacag cgatatgcac gcaggcaatt tatcatttta tttatctgaa ccaccatttg
      481 cgctgacgcc cgtctacgac atgctgccga tggcctatgc accaaacagc gccggaatgc
      541 tgcgtgatgc tgccattgag gtgaagtttg atcttaacgt cagtaaaagc gcatggttaa
      601 cggcgatccc gctggcgcag cagttctggc aaacggtcgc tggagatccg cgtatcagcg
      661 aggcgtttcg ccacattgcg caggaaatgc cggaaaaaat ccggcaaatc gaagggaaag
      721 ttgcccgcat gggcgggtaa ctaccgtacc gctcccgcta tccacgccga caactcccgt
      781 agctcttttt cctgttcagg gaagtcaacc aacagcgctt cacactcttg ttgcagcatg
      841 tcagcgcggt ataggcagcc ttgcagtcgt ccggcgagag cttccagagg tgcggggttg
      901 aggctatcgg taaacacctg ggtgcgggtg atatggcctt tttcgacgtc gaaatgcagt
      961 tctacgccgc cccaggtaaa gcgttcatcc agcagatgcg aaaacgccgg agcctgaccg
     1021 aagttccatt cccagctact ctggcgggca aaggtttcgg caaagttcgg caagtctggc
     1081 gttttgtccg gtgagatgat ttccgcttct gtgcgctcgc cataatgtgc gaaaaaggtt
     1141 tcggatatgg cctcgcaaac ctgctcatgg gtaattcccg gcagcaattc ggtcagattg
     1201 gtcacgcggg aacgtaccga cgtaatgcct ttcgccgcca gtttcttttt atccgggttg
     1261 agatagtttg ccaggcggct aaggt
//
LOCUS       DAFATA010000267         1245 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFATA010000267 DAFATA010000000
VERSION     DAFATA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.270"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..167)
                     /locus_tag="LGJ20_004902"
     CDS             complement(<1..167)
                     /locus_tag="LGJ20_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HBK2388871.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFG"
     gene            185..1165
                     /locus_tag="LGJ20_004903"
     CDS             185..1165
                     /locus_tag="LGJ20_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBK2388872.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
BASE COUNT          277 a          288 c          385 g          295 t
ORIGIN      
        1 tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc aggttttgtt
       61 ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt tgcagccgct
      121 gagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt ccgaggatct
      181 tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct cggatctaca
      241 cctgtgcagc gcctggcccg cacgatggcg cattcgcggc agaatggaag ctgcgccgtt
      301 tgatgcgccg gacgtcgaag agctactgcg ggagtggctg gatgacgatc agcgggcaat
      361 attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc agcggttgcg
      421 tggcagtgcg ttcgcgcaac ggcaaggtat ttctctggca ttacggttgt taccttcgca
      481 ctgtccacag ctcgaacagc ttggtgcgcc accggtattg ccggaattac tcaagagcga
      541 gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca cgctggcggc
      601 gatggttggc tatcttaatc aacatgccga tgcgcatatt ctgacgctgg aagatcctgt
      661 ggaatatctc tatgccagcc agcgatgttt gatccagcag cgggaaattg gtttgcactg
      721 tatgactttc gcatcggggt tgcgggctgc attgcgggaa gatcctgatg tgattttgct
      781 cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag aaaccgggca
      841 tttggtgctg gcaacattac atacgcgtgg tgccgcgcag gcagttgagc gactggtgga
      901 ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta gtttacgggc
      961 cgtgttgtca caaaagctgg aagtggataa acaggaagga cgcgtggcgc tgtttgaatt
     1021 attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc accagttacc
     1081 gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga gttatcagca
     1141 gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt tatagggcct
     1201 ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcag
//
LOCUS       DAFATA010000268         1181 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFATA010000268 DAFATA010000000
VERSION     DAFATA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.272"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..602)
                     /gene="gltX"
                     /locus_tag="LGJ20_004904"
     CDS             complement(<1..602)
                     /gene="gltX"
                     /locus_tag="LGJ20_004904"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK2388873.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFERYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRG"
     gene            861..936
                     /locus_tag="LGJ20_004905"
     tRNA            861..936
                     /locus_tag="LGJ20_004905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:894..896,aa:Val,seq:tac)
     gene            981..1056
                     /locus_tag="LGJ20_004906"
     tRNA            981..1056
                     /locus_tag="LGJ20_004906"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Val,seq:tac)
     gene            1103..1178
                     /locus_tag="LGJ20_004907"
     tRNA            1103..1178
                     /locus_tag="LGJ20_004907"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Val,seq:tac)
BASE COUNT          286 a          318 c          287 g          290 t
ORIGIN      
        1 tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac acagaagtta
       61 taggttgggg aaccatcggt acggcggata atcagatcat ccagctcctg gttgctgaac
      121 tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg attagcaaaa
      181 cgcacgacac acggttcatc atcagcatga tgctcatggc tgtggcggca gcgaccgtca
      241 taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag gcgctcttta
      301 gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac cgcattgtag
      361 cgctcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct cagccagttc
      421 atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc ggtgtcttca
      481 atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta aagagcagta
      541 cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt tttgattttc
      601 atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga aaaattgagt
      661 gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc taccttacgc
      721 attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat cttgcttaaa
      781 tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa ctctccctat
      841 aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc ccttacaagg
      901 agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag tctccgccgt
      961 gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc ccttacaagg
     1021 agggggtcgg cggttcgatc ccgtcatcac ccaccacttt ctcgccagct aaatttcttg
     1081 taaaaatgtg aagtaccgaa gtgggtgatt agctcagctg ggagagcacc tcccttacaa
     1141 ggagggggtc ggcggttcga tcccgtcatc acccaccact t
//
LOCUS       DAFATA010000269         1178 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFATA010000269 DAFATA010000000
VERSION     DAFATA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.273"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>1178
                     /locus_tag="LGJ20_004908"
     CDS             <1..>1178
                     /locus_tag="LGJ20_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:472..474,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBK2388874.1"
                     /translation="KLLRTRETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDP
                     DHPVNRGALCPKGAGLVDFIHSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKED
                     RDANYIAQNAEGVTVNRWLSTGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHG
                     PTVASLAPTFGRGAMTNHWVDIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLI
                     VIDPRFTRTAAVADYYAPIRSGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVRE
                     DYGFEDGLFTGYDAEKRKYDKSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYT
                     PDVVENICGTPKDAFLKVCEYIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQL
                     L"
BASE COUNT          289 a          331 c          309 g          249 t
ORIGIN      
        1 aaactgctgc gcacccgcga aacccgtaat acctgcacct attgttccgt aggctgtggg
       61 ctgttgatgt acagcctcgg tgacggagca aaaaacgcca aagcatctat cttccatatc
      121 gaaggcgatc cggatcaccc ggtcaaccgc ggtgcacttt gtccgaaagg cgctggcctg
      181 gtggatttca tccactccga aagccgtctg aagtttccgg aataccgtgc gccaggttct
      241 gataaatggc agcaaatcag ttgggaagag gcgtttgatc gcatcgccaa actgatgaaa
      301 gaagaccgcg atgctaacta cattgcgcaa aacgccgaag gcgtgactgt taaccgctgg
      361 ctctccaccg ggatgctgtg tgcttccgcg tcgagtaacg aaaccggcta tttaacgcaa
      421 aaattctccc gcgcgctggg tatgctcgcg gtcgacaacc aggcgcgtgt ctgacacgga
      481 ccaacggtag caagtcttgc tccaacattt ggtcgcggtg cgatgaccaa ccactgggtc
      541 gacatcaaga acgccaacct cgtcgtggtg atgggcggta acgccgctga agctcacccg
      601 gtcgggttcc gctgggcgat ggaagccaaa attcacaacg gcgcgaagct gattgtgatc
      661 gatcctcgct ttacgcgtac ggcggcggta gctgactact atgcccctat tcgttccggt
      721 actgacattg ctttcctgtc aggcgtattg ctgtacctgc tgaacaatga aaaattcaac
      781 cgcgaataca ccgaagccta taccaacgcc agcctgatag tgcgtgagga ttacggcttt
      841 gaagatggcc tgttcaccgg ctacgacgcg gaaaaacgca agtacgataa atcctcctgg
      901 acttatgaac tggacgaaaa cggcttcgcc aaacgcgata ccacgctgca acatccgcgc
      961 tgcgtgtgga acttgctgaa acagcacgtt tcccgctaca cgccagatgt ggttgaaaac
     1021 atctgtggta cgccaaaaga cgcgttcctg aaagtctgcg aatacatcgc agaaaccagt
     1081 gctcacgata aaactgcctc gttcctgtat gccctcggct ggacgcaaca ctccgttggt
     1141 gcgcaaaaca ttcgtacgat ggcgatgatc cagctgct
//
LOCUS       DAFATA010000270         1176 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFATA010000270 DAFATA010000000
VERSION     DAFATA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.274"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>1176
                     /locus_tag="LGJ20_004909"
     CDS             <1..>1176
                     /locus_tag="LGJ20_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HBK2388875.1"
                     /translation="IFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNN
                     IDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEG
                     KVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYC
                     ISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISP
                     VYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNE
                     SHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGILQIKTSSGAETSA
                     FIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSP
                     "
BASE COUNT          309 a          298 c          301 g          268 t
ORIGIN      
        1 catttttttc cccgcccttg agcaaaatat gatgggtgcg gtgttaatta acgaaaatga
       61 tgaagtgatg tttttcaacc ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt
      121 cattggcaat aacattgata tgctgattcc gcgggatttg cgtcctgcgc atcctgaata
      181 cattcgtcat aaccgtgaag gcggtaaagc gcgtgtcgag gggatgagtc gggagctgca
      241 gctggagaaa aaagacggca gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc
      301 cgaggggaaa gtttattacc tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa
      361 agaacagact cgacaattga ttattgccgt tgaccatctc gaccgaccgg tgattgtcct
      421 cgatccggaa cgccatattg tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg
      481 cattagcgaa gccagcggta tgcagcccga tacactcctg aacattcctg aattccctgc
      541 cgataaccgc attcgtttac aacagttgct atggaaaacc gcccgcgatc aggacgaatt
      601 tctgctgttg acgcgcaccg gtgaaaaaat ctggattaaa gcctctatca gcccggttta
      661 tgacgtgctc gcgcatctgc agaacctggt aatgactttc tcggatatca ccgaagaacg
      721 gcaaatccgc cagcttgaag gcaatattct cgccgccatg tgcagcagcc cgccatttca
      781 tgaaatgggg gaaatcattt gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc
      841 gctgttcgca ctgcgcaacg ggatgccgat acactgggcg tcatcttcgc acggtgcaga
      901 agttcaaaat gcgcaaagct ggtcagcgac cattcgtcag cgtgatggcg cgcctgcggg
      961 gatcctgcaa attaaaacct cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc
     1021 agatatcagt cagcatatgg ccgcgctggc gctggaacag gaaaaaagcc gccagcatat
     1081 tgaacaactc atccaatttg atccgatgac cggtctgcca aatcgcaata acctgcacaa
     1141 ttacctcgat gacctggtcg acaaagccgt ctctcc
//
LOCUS       DAFATA010000271         1169 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFATA010000271 DAFATA010000000
VERSION     DAFATA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.275"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            3..401
                     /locus_tag="LGJ20_004910"
     CDS             3..401
                     /locus_tag="LGJ20_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HBK2388876.1"
                     /translation="MALVALGWQSWRLNSASHTIETQRAALKSKAQELTKKNSQLIGL
                     SILAETNNREQARLYAEAEQTSALLRQRQRRIEELKRENEDLRRWADTPLPADIIRLR
                     ERPALTGGAAYRQWLSASDAVSAGAGCAAH"
     gene            497..928
                     /locus_tag="LGJ20_004911"
     CDS             497..928
                     /locus_tag="LGJ20_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388877.1"
                     /translation="MNKPQSLRHAINKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTNGSVSSVEAIAEPDEPEEMWTVKRG"
     gene            921..>1169
                     /locus_tag="LGJ20_004912"
     CDS             921..>1169
                     /locus_tag="LGJ20_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBK2388878.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFTKLRTTKYLKT"
BASE COUNT          305 a          308 c          342 g          214 t
ORIGIN      
        1 cactggcact cgttgcgctg ggctggcagt cgtggcggct taacagcgcc agccacacca
       61 tcgaaacgca gcgcgcggcg ctgaaaagta aagcgcagga actgacgaag aaaaacagcc
      121 agctgatcgg tctatccatt ctggctgaaa ccaacaaccg ggagcaggcg cggctctacg
      181 ccgaagcaga acagaccagt gcactgctga gacaacgaca acgccggatc gaggaactga
      241 aacgtgagaa cgaggattta cgccgctggg ctgatactcc tttgcctgct gacattatcc
      301 ggctgcggga acgtccggca cttaccggag gtgcagctta ccgtcagtgg ttgtccgcga
      361 gtgacgccgt gtcggctgga gcaggctgcg ccgcgcacta acggtgatct gaacgcattg
      421 ctggatgaaa cggaggccgc ctgggcggtc tgtgcagaca aagtggacat gattattgcg
      481 tgtcaggagc gaaacagtga acaaaccaca gtccctgcgc cacgccatca ataaagcagt
      541 gccttatgtc cgcaataacc cggacaaact gcatctgttt gtggataacg gttcgctggt
      601 tgccacgggg gccagctcca tgtcatggga gtaccgctat accctgaacg tggtgataga
      661 ggatttcagc ggcgaccaaa atttgctgat ggccccggtt ttactgtggc tgcgggataa
      721 ccagcccgat gccatcaata acccggcgtt acgggaaaag ctattcacct ttgaggtgga
      781 tattctgcgc aacgatgtct gtgatatcag ccttaacctg caactgacgg aacgtgtgct
      841 ggtcagcact aacggcagtg tgtcgagcgt tgaagctata gcggaacctg atgaacctga
      901 agaaatgtgg acggtgaaac gtggctgaat tgcagaaagt ggacgactgg ctgagtgcct
      961 tgctggcgaa tctggaacca gccgccagaa gccgcatgat gcgccagctg gcgcaggaac
     1021 tgcgccggac acagcagcag aatatcagga tgcaacgcaa tcctgatggc agcagctatg
     1081 aaccgaggcg agtaacagca cgcagtaaaa aaggccgcat caaacggcag atgtttacaa
     1141 aacttcgcac cacaaaatac ctgaaaacc
//
LOCUS       DAFATA010000272         1155 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFATA010000272 DAFATA010000000
VERSION     DAFATA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.276"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(202..>1155)
                     /locus_tag="LGJ20_004913"
     CDS             complement(202..>1155)
                     /locus_tag="LGJ20_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK2388879.1"
                     /translation="ARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVDAVSNAI
                     RRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEAMVRIDMSEFMEKHS
                     VSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLT
                     DGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKELVLGVVSHNFRPEFINRID
                     EVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDEALKLLSENGYDPVYGARP
                     LKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAVQ"
BASE COUNT          295 a          327 c          259 g          274 t
ORIGIN      
        1 aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt ggtggcgcat
       61 tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg ttcgctcatt
      121 tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca aaaacgagcc
      181 ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat taacttccag
      241 gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca gcgggttttc
      301 gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat cgtaaccgtt
      361 ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac gttcttccag
      421 acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct gttcacccag
      481 cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga agttatggct
      541 taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga agcgttcctg
      601 aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga agtcgaccgt
      661 tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa tgttgaagac
      721 atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac gacgacgcac
      781 agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg caccaaccag
      841 acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca tcgcctcgtc
      901 gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca cacccgttgg
      961 gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg cacggctacg
     1021 gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc gatggtgcag
     1081 ttcttgttcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag aaaccggaat
     1141 ccccgtccaa cgcgc
//
LOCUS       DAFATA010000273         1155 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFATA010000273 DAFATA010000000
VERSION     DAFATA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.277"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>1155
                     /gene="chiA"
                     /locus_tag="LGJ20_004914"
     CDS             <1..>1155
                     /gene="chiA"
                     /locus_tag="LGJ20_004914"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HBK2388880.1"
                     /translation="KIYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNT
                     LSCEKSGSSSSSNSNTPASNTPANGSSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFN
                     GAVYKNAWWVASSNCPGDAKSNDASNPWRYVRAATATEISETSNPQSCTSAPQPSPDV
                     KPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANC
                     PGDAKENDASNPWRYVRAATATEISQYGNPGSCSVKPDNNGGAVTPVDPTPETPVTPT
                     PDNSEPSTPADSVNDYSLQAWSGQEGSEIYHVIFNGNVYKNAWWVGSKDCPRGTSAEN
                     SNNPWRLERTATAAELSQYGNPTTCEIDNGGVIVADGFQASKAYSADSIV"
BASE COUNT          312 a          289 c          297 g          257 t
ORIGIN      
        1 caaaatttat gaaaatgcct ggtgggtttc ttctacaaat tgcccgggaa aagcgaaagc
       61 aaatgatgca actaacccgt ggcgtttaaa gcgtaccgca acagctgctg aaattagtca
      121 gtttggcaat acactttcct gcgaaaagag cggcagctca tcttcttcaa attcaaatac
      181 gcctgcatcc aatacgccgg ctaatggcag ttcggctaca ccagcacagg gcactgttcc
      241 gtctaattct tctgtagttg cctggaataa acagcagggc ggtcagacct ggtatgtcgt
      301 ctttaatggt gcggtatata aaaatgcctg gtgggtagcc tcttctaact gtccgggtga
      361 tgcgaaaagc aatgatgcca gcaacccatg gcgttatgtt cgtgccgcta cggcaacgga
      421 aatctcagaa accagtaatc cacagtcctg tacttcagca ccacagcctt caccggatgt
      481 gaaaccggca ccggacgtta aaccggctcc tgatgttcag ccagccccag ctgataagtc
      541 aaacgacaac tatgctgtag tagcctggaa aggtcaggaa ggttcttcta catggtacgt
      601 tatctataac ggcggcattt ataagaacgc ctggtgggta ggcgcggcaa attgcccagg
      661 cgatgcgaaa gaaaacgatg ccagtaaccc atggcgttat gttcgcgcgg caacggcaac
      721 agaaatcagc cagtatggta accctggctc ctgttccgtt aagccggata ataatggcgg
      781 tgctgtgact ccggttgatc caactccgga aacaccggtg accccaactc cggataacag
      841 cgagccatca acaccagcgg atagcgttaa cgattactca ttgcaagcgt ggagcggcca
      901 ggaaggtagc gaaatttacc atgttatttt caatggtaat gtttacaaga acgcctggtg
      961 ggttgggtct aaagattgcc cacggggtac cagcgctgaa aactccaata acccatggcg
     1021 tctcgagcgt acagctaccg ctgcggaatt gagtcagtac ggtaacccga ctacctgtga
     1081 aattgataac ggcggcgtca ttgttgcgga tggtttccag gccagcaaag cgtacagcgc
     1141 ggacagcatc gtaga
//
LOCUS       DAFATA010000274         1150 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFATA010000274 DAFATA010000000
VERSION     DAFATA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.278"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..600
                     /locus_tag="LGJ20_004915"
     CDS             <1..600
                     /locus_tag="LGJ20_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HBK2388881.1"
                     /translation="VSGDEAFYTFLYLTRDTFSPWENLALLLQNYDNIDFQGLAPIVR
                     DFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAISPTLIGSLVVMGGALFIPLG
                     AIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIFNMIVLAREGLDSFVSRVVF
                     FIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG"
     gene            603..>1150
                     /gene="rffM"
                     /locus_tag="LGJ20_004916"
     CDS             603..>1150
                     /gene="rffM"
                     /locus_tag="LGJ20_004916"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBK2388882.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLV"
BASE COUNT          253 a          278 c          326 g          293 t
ORIGIN      
        1 gtgagcggcg atgaagcgtt ctatacgttt ctctatctca ctcgcgacac cttctcgccg
       61 tgggagaatc tggcgttgct gttgcagaac tacgacaaca tcgacttcca gggcctggct
      121 ccaattgtcc gcgatttcta tgtctttatc ccttcctggc tgtggccggg tcgcccgagt
      181 atggtgctga actcagccaa ctactttacc tgggaagtgc tgaataacca ctccggactg
      241 gcgatctcgc ctacgcttat aggctcactg gtggtgatgg gcggcgcgtt gttcatcccg
      301 ctcggggcga tcgtggttgg tctgatcatc aaatggttcg actggctgta tgagctgggc
      361 aaccgcgagc ctaatcgcta taaggctgcg atattgcaca gtttctgctt tggagcgatc
      421 ttcaatatga tcgtgctggc gcgtgaaggg ctggattcgt ttgtctcacg cgtggtcttt
      481 tttatcgtgg tcttcggcgc atgtctgatg atcgcaaaac tgttgtactg gctttttgaa
      541 agcgccggac tcattcataa acgtacaaaa tcatcgctcc ggacgcaggt tgaaggataa
      601 caatgaataa caacaccacg gcaccaacct atacgctgcg tggcttacag ttgattggtt
      661 ggcgtgatat gcagcacgcc ctcgattatc tgtttgctga cgggcagctt aagcagggaa
      721 cgctggttgc cattaatgct gaaaaaatgc tgactattga agataacgcc gaggtcaggg
      781 agttaattaa cgctgccgaa tttaaatatg cggatggcat cagcgttgta cgttcagtac
      841 gtaaaaagta cccgcaggcg caggtttccc gcgttgccgg tgccgatctc tgggaagagc
      901 tgatggcgcg cgcaggcaaa gaagggacgc cggtatttct tgtgggcggt aaacctgaag
      961 tgctggcgca aactgaagct aaactgcgca accaatggaa tgtgaatatc gttggcagtc
     1021 aggatggtta ttttaaaccc gagcagcgtc aggcgctgtt tgaacgcatt catgccagcg
     1081 gagcgcaaat cgtcaccgtt gcgatgggat cgccaaagca ggagatcttc atgcgcgact
     1141 gccgtctggt
//
LOCUS       DAFATA010000275         1149 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFATA010000275 DAFATA010000000
VERSION     DAFATA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.279"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..421
                     /locus_tag="LGJ20_004917"
     CDS             <1..421
                     /locus_tag="LGJ20_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBK2388883.1"
                     /translation="LNNKEITTIIRRNGFRFWGNRTPETNAYIFEVYTRTAQVLADSI
                     AEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAGKLIGAECWYDVVDNSTTDLRQGR
                     VRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLGGA"
     gene            421..933
                     /locus_tag="LGJ20_004918"
     CDS             421..933
                     /locus_tag="LGJ20_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBK2388884.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVREPFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            988..>1149
                     /locus_tag="LGJ20_004919"
     CDS             988..>1149
                     /locus_tag="LGJ20_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2388885.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSL"
BASE COUNT          291 a          273 c          335 g          250 t
ORIGIN      
        1 cctcaacaac aaagaaatca cgaccattat tcgtcgcaac gggttccgct tctggggcaa
       61 ccgcacaccg gaaacgaacg cctacatctt tgaggtgtat acccgaaccg cacaggtgct
      121 ggctgattca attgcggaag cgcagtttga aaccatcgac agtccactga cgcctgcgaa
      181 cgtgaaggat gttatcagtg ccatcagggc aaaactggat tcactggtga ctgccgggaa
      241 actgattggc gcggagtgct ggtatgacgt ggtggataac agcaccacgg atttacgtca
      301 ggggcgtgtg cgtattcgct acaaatatac gcccgttccg ccactggaag acatggagct
      361 ttaccagacg tttactgatg aatactttga acccgcattt gcggtgctgg gaggtgcctg
      421 atggctgtgc caaaacatct tcgctttttt acgctgtttg tggatggtga aaacgaagtg
      481 ggtaaggtga cgtccgtcac tttgcctaag ctgacgcgca aaaccgacag ctaccggggt
      541 ggtggcatga tgggggcggt aagtattgat ctcggcctgg acgactccgc gcttgatgcg
      601 agctttgtca tggggggcgc agttcgtgag ccgttcctta agtatggcgg cacgattgac
      661 ggcacgctgc tgcgttttgc gggtgaatac tacaccgatg cagaaagcga cctgtatgaa
      721 gtcgaaatgc gcggacgtgt gacggaaatt gatatggggg aagccaaaca gggcgaagcc
      781 acatcacaca cttacgccat taaaaacacc tactacaagc tgagcgttaa cgatcgcccg
      841 ttgtgggaga ttgacctgct gaacttcatt taccggaagg acggcaagga cattgtgccc
      901 gatcgcatcc gttccgcgct tgggcttggc tgataagtaa tatgcaggcg gcgcagtgcg
      961 tcgcctctga ctgaaaggag tttcctgatg aaagagacga aaaacatcga taccgaaaat
     1021 acggtagttg ctgacactgt gaaagaaacc agcgagcgtg gcgtaaaact tacccaacca
     1081 attgagcgag gcggcgaaaa aatcacgtat gtggagatca ccggggctat tgagcaggct
     1141 ggatctctg
//
LOCUS       DAFATA010000276         1148 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFATA010000276 DAFATA010000000
VERSION     DAFATA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.280"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            1..525
                     /locus_tag="LGJ20_004920"
                     /pseudo
     CDS             1..525
                     /locus_tag="LGJ20_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            579..806
                     /gene="pptA"
                     /locus_tag="LGJ20_004921"
     CDS             579..806
                     /gene="pptA"
                     /locus_tag="LGJ20_004921"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBK2388886.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(810..>1148)
                     /locus_tag="LGJ20_004922"
     CDS             complement(810..>1148)
                     /locus_tag="LGJ20_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388887.1"
                     /translation="GVAATNWTWAVGSVSGREEDKFNCYGIPVVKGPVLGLPLVEEKC
                     LAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTF
                     VTSGKRVTAG"
BASE COUNT          356 a          264 c          245 g          283 t
ORIGIN      
        1 cctacaaaat ttcatgagtg ctttattaac tggatgcgtg attgtcattc ttcagatgat
       61 aaagacgtca ttgcaattga tggaaaaacg ctccggcatt cttatgataa gagtcgccgc
      121 aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt catcggacag
      181 atcaagacgg atgataaatc taatgagatt acagctatcc cagaacttct taacatgctg
      241 gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga tatagcagag
      301 aagatacaaa aacagggagg tgattattta ttcgcggtga aaggaaacca ggggtggcta
      361 aataaagcct ttgaggaaaa atttccgctg aaagaattaa ataatccaga gcatgacagt
      421 tacgcaattt gtgaaaagag tcacggcaga gaagaaatcc gtattcatat tgtttgcgat
      481 gtccctgatg aacttattga tttcacgttt gaatggaaag gactgaaaat tcacaatgtg
      541 tgttaatttt actgacatct ttcaaaagga gcacaatcat gccgcacatc gacattaaat
      601 gctttccgcg tgaactggac gaacaacaaa aagcagcact tgctgcagat attaccgacg
      661 ttattattcg ccatctgaac agtaaagaca gttcgataag cattgctcta cagcagattc
      721 aaccagaatc ttggcaagct atctgggatg ccgaaatcgc gccccaaatg gaggctttga
      781 taaagaaacc cggttacagc atgtaatgct taacctgccg taacacgctt gccgctagta
      841 acaaacgtgc cagcacctaa atgatgcaac gtattgagct tatcatcatc aaactgccag
      901 cggccttcga caaatacccg tgcgtctgcc gctgctgata ccacctcgcc aaacagcgtg
      961 tcgtattttt gttgcgcaga agtcgcaggt agcaggcggc actccatcca cgccaggcat
     1021 ttctcttcaa caagtggcaa accaagcacc ggacctttca ccaccggaat gccatagcaa
     1081 ttaaatttat cttcttcacg acccgacacg cttcccactg cccacgtcca gttagttgct
     1141 gcaacgcc
//
LOCUS       DAFATA010000277         1127 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFATA010000277 DAFATA010000000
VERSION     DAFATA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.281"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..784
                     /locus_tag="LGJ20_004923"
     CDS             <1..784
                     /locus_tag="LGJ20_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBK2388888.1"
                     /translation="SCVIGAFNANYHLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAI
                     DYALHGPVNPSLLVITDTDNDKLSYARKHYPSEPQTLIHYLNATDAAFDTLMALSGGH
                     GFDDIFVFVPNEGLVTLASSLLATDGCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGT
                     SGGNTDDMRAAVKLIEEKKVQAAKVVTHILGLNAAGETTLELPAVGGGKKLVYTGKYL
                     PLTSLTQIQDQALAAILARHQGIWSGEAEQYLLTHAEAISHD"
BASE COUNT          274 a          283 c          300 g          270 t
ORIGIN      
        1 ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt caggaaggta gttataacca
       61 cacgatgggg attcgcccgc aagggcgcat gctgatcctc ggcggcaccg gaccaatggg
      121 actgttggcg attgattatg cgctacatgg acccgttaac ccgtcgctgc tcgtcattac
      181 cgataccgac aacgataaat tgagttatgc gcgcaagcac tatccatcag aaccgcaaac
      241 actgattcat tatctcaatg ccaccgatgc agcatttgat acgttaatgg cgctgagtgg
      301 cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg tgactctcgc
      361 ctcttccttg ctggcgacag atggttgcct gaatttcttc gccggaccgc aggataaaca
      421 tttcagcgcg ccaattaatt tctacgatgt gcattatgca tttacccact acgtgggtac
      481 gtcaggcggc aataccgacg acatgcgcgc agcggtcaaa ctgattgaag agaaaaaagt
      541 gcaggccgca aaagtggtaa cacatattct tgggctgaat gccgcgggcg aaaccacgct
      601 tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat acctgccgct
      661 gacgtcactc acgcagattc aggatcaagc actggcggcg attctggcgc gtcatcaggg
      721 gatctggtcc ggtgaggcgg agcaatatct gctcactcat gcagaggcaa tttcccatga
      781 ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg ccgcggcaac ttcgcgacct
      841 gatgtgcggc tgattgctga agcggcccaa tgtgcacgag aaggataaaa aaacatcagc
      901 gccttaaggg gcgctcaaat tggtggcaaa gtacacattg tttatgccgg atgcggaata
      961 aagccttatt cggcctacat aatctcgaaa attcaatata ttgcagttat ctggtaggcc
     1021 tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc agtctcagcg cccggtgcaa
     1081 tacgtgacat cgtgagccat gtcagtaagt ggttaaattc gcagtgg
//
LOCUS       DAFATA010000278         1125 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFATA010000278 DAFATA010000000
VERSION     DAFATA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.282"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..1087)
                     /locus_tag="LGJ20_004924"
     CDS             complement(<1..1087)
                     /locus_tag="LGJ20_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HBK2388889.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLCATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDLQTGEVSDLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCT"
BASE COUNT          275 a          312 c          283 g          255 t
ORIGIN      
        1 cagtacaacg acgtgaaaaa ctcagccggt ttgttgccgt ttgcaagaaa ggtttcagga
       61 tcgctgactt ccacagcgtt aagcctggcg cccataaata accattacgc tgttgcagat
      121 gtcgacgttg taattgcagc cattgcagca cggtaaatag atcgcttact tctcccgttt
      181 gcagatcgat atagcgacag taacgcaggt atgcagcggc aaaaagttcc tccagcgtgg
      241 cagaaccgcg tcgggcagat aacaaagcgg actgtggatg acgatcgtcg gttaagcccc
      301 aacctgcata ccagggctgg ccgaagcatg ttactggttt tcctgccagc aaggcttcaa
      361 agccatattg ggaggtcacg acgtaaaccc gggaaacgtg tcgcaacagc gactgcgggc
      421 tgacattctc ggcaatcaaa cgtactcgtt gcgtggcgca cagatcagcg aaataacctg
      481 ttttctttcc ttccaggaca tccggatgca ccttcaccca aatttcggct tgaggatttt
      541 ccgccatcgc ggcttccagc atggcagcaa actcatgcgg gccagcattg ccatacgtca
      601 ctgacatatc attaaatgtc tgatcgacaa ccagaacgat gtctgaacgt tctgactcat
      661 cagccacaaa cgcaggtgcc aggttatatt tcgacaaatc cccggtcacg atggtgtgca
      721 tcgcttctct ggcctggctt atcagagctg tatttccggc tttatcctgt accagtttct
      781 ccagtgctga aggcttgctg gcatcgtagt aaatgccaca atcatccacc accagagaaa
      841 gcggcggctc gccattgacg ccaagatcca gcgaacgcac aaatccatct tccagacgaa
      901 tgacgggttt tcccgctgct ttggcgatgg cgactggttt cgccgcgctg ggacgatgtc
      961 cccacacggc gatagcatca acttcttgcg gaacagggcg cagcagagaa agtttctggc
     1021 acggttgcgc cagaaatttc tccagatgcg gaatacgcca gatgccaggc gagtaaatgc
     1081 caatcatgcg ttttcagcca gttcctgtgc catcagggcg cgcac
//
LOCUS       DAFATA010000279         1121 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFATA010000279 DAFATA010000000
VERSION     DAFATA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.283"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..612
                     /locus_tag="LGJ20_004925"
     CDS             <1..612
                     /locus_tag="LGJ20_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBK2388890.1"
                     /translation="EMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERILAQ
                     SIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPK
                     QEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQLS
                     HQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            672..1061
                     /gene="mutT"
                     /locus_tag="LGJ20_004926"
     CDS             672..1061
                     /gene="mutT"
                     /locus_tag="LGJ20_004926"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBK2388891.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
BASE COUNT          312 a          241 c          327 g          241 t
ORIGIN      
        1 gaaatgtggg atattccggg gctgcaggaa cgtctgaaga acgatttcga cctcgattta
       61 ccaattgccg agtggctgga taaagaacca gaactgcatg aagagacgct gcgtgagcgc
      121 attctggcgc agtccatcga agtgtatcag cgtaaagaag aagtggttgg tgctgagatg
      181 atgcgtcact tcgagaaagg cgtcatgctg caaacgcttg actccctgtg gaaagagcac
      241 ctggcagcga tggactatct gcgtcagggt atccacctgc gtggctatgc acagaaagat
      301 ccgaagcagg aatacaaacg tgaatcgttc tccatgtttg cagcgatgct ggagtcgtta
      361 aaatatgaag ttatcagcac gctgagcaaa gtccaggtac gtatgcctga agaggttgag
      421 gagctggaac aacagcgtcg tatggaagcc gagcgtttag cgcaaatgca gcagcttagc
      481 catcaggatg acgactctgc agccgcagct gcactggcgg cgcaaaccgg tgagcgcaaa
      541 gtaggacgta acgatccttg cccgtgcggt tctggtaaaa aatacaagca gtgccatggt
      601 cgcctgcaat aaaagctaac agttgaagta aaaggcgcag gattctgcgc cttttttata
      661 ggtttaagac aatgaaaaag ctgcaaattg cggtaggtat tattcgcaac gagaacaatg
      721 aaatctttat aacgcgtcgc gcagcagatg cgcacatggc gaataaactg gagtttcccg
      781 gcggtaaaat tgaaatgggc gaaacgccgg aacaggcggt ggtgcgtgaa cttcaggaag
      841 aagtcgggat taccccacaa catttttcgc tatttgaaaa actggaatat gaattcccgg
      901 acaggcatat aactctgtgg ttctggctgg tcgaaagctg ggaaggggag ccgtggggta
      961 aagaagggca acccggtgag tggatgtcgc tagtcggtct taatgccgat gattttccgc
     1021 cagccaatga accggtaata gcgaagctta agcgtctgta ggtcggataa ggcgttcacg
     1081 ccgcatccga catttgcaca agatgcctga tgcgacgctg t
//
LOCUS       DAFATA010000280         1100 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFATA010000280 DAFATA010000000
VERSION     DAFATA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.284"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>1100)
                     /locus_tag="LGJ20_004927"
     CDS             complement(<1..>1100)
                     /locus_tag="LGJ20_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK2388892.1"
                     /translation="LVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAILG
                     YVWPPVQHAIHAGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNA
                     AGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLL
                     SVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAID
                     YALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEA
                     NSNTEEGLNQLATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGV
                     VKLNKQTIQVIVGAKAESIGDAMKKVVARGPVA"
BASE COUNT          289 a          317 c          294 g          200 t
ORIGIN      
        1 gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg
       61 ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc acccagacgt
      121 ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag acgggtgata
      181 caggcgtcaa ttgctttcag gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc
      241 agttgattga ggccttcttc agtgttgctg ttggcttctt cagtaacgat ctcgtcttct
      301 ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg
      361 aagtagatag cgaagaagac aacacccatc accagcagca tccagacgtt ctggctggcg
      421 gccggcaggt tatacatcaa cgcgtagtcg atagcccccg cagagaaaga gaagccagca
      481 tggataccca gcagtgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg
      541 tacagcagcg gtgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg
      601 aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt
      661 gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcataat cgggaagaag
      721 ccggacatga acatccccgc ggtgccgtca ccggcataga agcggttgat atcaccgtgg
      781 aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc
      841 acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc
      901 agcgcgcccg cagaaacgat ccactcgcct cctgcatgga tagcgtgctg taccggcggc
      961 cagacgtaac caagaatggc cgccagcacc aggcagaaga agccggtggc aatcggcaca
     1021 aagcgtttgc cgccgaagaa actcaggaag tccggcagtt taatatcgga ccaacggtta
     1081 taggctgcgc caccaaccag
//
LOCUS       DAFATA010000281         1075 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFATA010000281 DAFATA010000000
VERSION     DAFATA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.285"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>1075
                     /gene="secA"
                     /locus_tag="LGJ20_004928"
     CDS             <1..>1075
                     /gene="secA"
                     /locus_tag="LGJ20_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704405.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK2388893.1"
                     /translation="DGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLA
                     SITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTE
                     AEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAA
                     IVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDA
                     VLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSG
                     MMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQR
                     NELLDVSDVSETINSIREDVFKAT"
BASE COUNT          281 a          265 c          296 g          233 t
ORIGIN      
        1 gatggtgaag ttatcatcgt tgacgaacac accggtcgta ccatgcaggg ccgtcgctgg
       61 tccgatggtc tgcaccaggc tgtggaagcg aaagaaggtg tgcagatcca gaacgaaaac
      121 caaacgctgg cttcgatcac cttccagaac tacttccgtc tgtatgaaaa actggcgggg
      181 atgaccggta ctgctgatac cgaagctttc gaatttagct ccatctacaa gctggatacc
      241 gtcgttgttc cgaccaaccg tccaatgatt cgtaaagatc tgccggacct ggtctacatg
      301 actgaagcgg aaaaaattca ggcgatcatt gaagatatca aagaacgtac tgcgaaaggc
      361 cagccggtgc tggtgggtac tatctccatc gaaaaatcgg agctggtgtc aaacgaactg
      421 accaaagccg gtattaagca caacgtcctg aacgccaaat tccacgccaa cgaagcggcg
      481 attgttgctc aggcaggtta tccggctgcg gtgactatcg cgaccaatat ggcgggtcgt
      541 ggtacagata ttgtgctcgg tggtagctgg caggcagaag ttgccgcgct ggaaaatccg
      601 accgcagagc aaattgaaaa aattaaagcc gactggcagg tacgtcacga tgcggtactg
      661 gaagcaggtg gcctgcatat catcggtacc gagcgtcacg aatcccgtcg tatcgataac
      721 cagttgcgcg gtcgttctgg tcgtcagggg gatgctggtt cttcccgttt ctacctgtcg
      781 atggaagatg cgctgatgcg tatttttgct tccgaccgag tatccggcat gatgcgtaaa
      841 ctgggtatga agccaggcga agccattgaa cacccgtggg tgactaaagc gattgccaac
      901 gcccagcgta aagttgaaag ccgtaacttc gacattcgta agcaactgct ggaatatgat
      961 gacgtggcta acgatcagcg tcgcgccatt tactcccagc gtaacgaact gctggatgtc
     1021 agcgatgtga gcgaaaccat caacagcatt cgtgaagatg tgttcaaagc cacca
//
LOCUS       DAFATA010000282         1007 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFATA010000282 DAFATA010000000
VERSION     DAFATA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.286"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..371
                     /locus_tag="LGJ20_004929"
     CDS             <1..371
                     /locus_tag="LGJ20_004929"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="with AtoC is a member of a two-component regulatory
                     system involved in the transcriptional regulation of the
                     ato genes for acetoacetate metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBK2388894.1"
                     /translation="DNELSPINADRELLKQVLLNILINAVQAISARGKIRIQTWQYSD
                     SQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGLGLALSQRIINAHQGDIRVASLP
                     GYGATFTLILPINPQGNQTV"
     gene            368..>1007
                     /locus_tag="LGJ20_004930"
     CDS             368..>1007
                     /locus_tag="LGJ20_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK2388895.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRVKGPFIKVNCAALPESLLESE"
BASE COUNT          270 a          261 c          249 g          227 t
ORIGIN      
        1 tggataatga attaagcccg attaacgccg atcgtgaact gctcaaacag gtactactga
       61 atatcctgat caatgccgtc caggctatca gcgcacgagg gaaaattcgc attcaaacct
      121 ggcaatacag cgactcacaa caggccattt cgatagagga caacggctgt ggcattgatc
      181 tctcgctgca aaaaaagatc ttcgatccct ttttcaccac caaagcctca ggaaccgggc
      241 ttggtctggc gttaagtcaa cgcatcatta atgcccatca gggtgatatt cgcgtcgcca
      301 gtttgccggg ctacggcgca accttcacgc ttattttacc gatcaacccg cagggaaatc
      361 agactgtatg actgctatta atcgcatcct tattgtggat gatgaagata atgttcgccg
      421 tatgctgagc accgcttttg cactacaagg attcgaaaca cattgtgcga acaacggacg
      481 cacagcatta cacctgtttg ccgatattca ccctgatgtg gtgttgatgg atatccgcat
      541 gccagagatg gacggcatca aggcactaaa ggagatgcgc agccatgaga cccggacacc
      601 cgttattctg atgacggcct atgcggaagt ggaaaccgcc gtcgaagcgc tacgctgcgg
      661 agccttcgac tatgttatta aaccgtttga tctcgatgag ttgaatttaa tcgttcagcg
      721 cgctttacaa ctccagtcaa tgaaaaaaga gatccgtcat ctgcaccagg cactgagcac
      781 cagctggcaa tgggggcaca ttctcaccaa cagcccggcg atgatggaca tctgcaaaga
      841 caccgccaaa attgcccttt ctcaggccag cgtcttgatt agcggtgaaa gcggcaccgg
      901 gaaagagttg attgccagag cgattcacta caattcgcgg cgggtaaagg ggccgttcat
      961 taaagtcaac tgcgcggcgc tgccggaatc gttgctcgaa agtgaac
//
LOCUS       DAFATA010000283         1006 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFATA010000283 DAFATA010000000
VERSION     DAFATA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.287"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..681)
                     /locus_tag="LGJ20_004931"
     CDS             complement(<1..681)
                     /locus_tag="LGJ20_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK2388896.1"
                     /translation="MADDRKMTDWHRKVLCNFDNAWSATKDMREQIIEAQRFVRVSGA
                     QWEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEA
                     LAEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISL
                     LPVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQ
                     YDWSTPDAI"
     gene            complement(681..>1006)
                     /locus_tag="LGJ20_004932"
     CDS             complement(681..>1006)
                     /locus_tag="LGJ20_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK2388897.1"
                     /translation="YAEAGFDMLPDHATWPDGGNAVEPGIAEIRDMMLDGRFKVFNTC
                     EPFFEEFRLYHRDENGKIVKLNDDILSAVRYGYMMRRFAIQMRDIKDPKEIDYSSYNI
                     PCGVG"
BASE COUNT          242 a          272 c          226 g          266 t
ORIGIN      
        1 gatggcatcg ggcgttgacc agtcatattg agtgccagtg tcatcacgag aaaggcttgc
       61 cgcgatggaa tcagggtatt cagcctcgaa cgctttaggc gtcatggaga acatttccat
      121 agcccacata gcatcagagc ggtcatattg cttgctgtcc tgatcgaaga aaacgcatgt
      181 cgctgggtcg taaacaggaa gaaggctgat gcgtcgctgc tcgttactcg gatccatttc
      241 atcttcgtaa tcggcacaca tgcggaaaca accgaatccg cccgttacag catcatcaaa
      301 tgcgttatca cacgcttcgc caccggatgt ttcctgatag tcagcgcgga atttgccgtt
      361 catcttttcg gctaacgctt ccgatgcctt atcgtcctta ggcctgaatt taacgctgat
      421 gcgattctgt cgatactcgc caatgatgcg atcacattca cgggcaatct tattcagttc
      481 aaagcgcggg taatgctcaa acctgccttc atcaaatgag taaccagcgt ttgtgctgcc
      541 ttcccactgt gcgccggaca cccggacgaa acgttgagcc tcaataatct gctcacgcat
      601 atccttcgtt gctgaccagg cattatcaaa gttgcacagc accttgcgat gccagtcagt
      661 catctttcta tcatcagcca tcatccaact ccgcaaggta tgttgtagct tgagtaatca
      721 atctctttag gatctttgat gtctcgcatc tgtattgcaa aacgcctcat catgtagcca
      781 tagcgaacag cagaaaggat gtcgtcattt agcttgacga tcttcccgtt ctcatcgcgg
      841 tgatacagac gaaactcttc aaagaatggc tcgcaggtgt taaatacctt gaaacgaccg
      901 tcgagcatca tgtcgcgtat ctctgctatc ccgggttcga ccgcattacc tccatcaggc
      961 catgttgcat gatctggcaa catgtcgaac ccagcttcgg cgtatt
//
LOCUS       DAFATA010000284         1006 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFATA010000284 DAFATA010000000
VERSION     DAFATA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.288"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..537
                     /locus_tag="LGJ20_004933"
     CDS             <1..537
                     /locus_tag="LGJ20_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HBK2388898.1"
                     /translation="VRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPLL
                     QKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVATE
                     TAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFN
                     GQEMSEEEFMSRAGRFVH"
     gene            complement(578..>1006)
                     /locus_tag="LGJ20_004934"
     CDS             complement(578..>1006)
                     /locus_tag="LGJ20_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK2388899.1"
                     /translation="ETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPP
                     KAAGIAMALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADEL
                     HGDYINDKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          300 a          219 c          226 g          261 t
ORIGIN      
        1 gtgcgccaat ttatcatcca gtataacatt gcaatgcaga agcagcttgc tgcacaccct
       61 gagttagcaa acgatgaagt tgctctgcaa gaagtgaatg ctgcattgtt caaagagtat
      121 ttaccgttat tacaaaaaag tgagccgacc attaaacaac cggtaaaatg gaagaacgca
      181 ctcggcgaac taaatgccaa tctggatatc agtattgccg acccagccaa atcttcatca
      241 tccacaaaca aagatattaa atcgctcaat tttgatgtga agttaccgct taatgtcgcc
      301 acagaaaccg caaaacagct taatttatct gaaggaatgg atgcggaaaa agcgcaaaag
      361 cgggctgata aacaaatcag cgggatgatg accttaggtc agatgtttca gttaatcacg
      421 attgacaaca ataccgcctc gctgcaactg cgttatacac cgggtaaagt tgtttttaac
      481 ggacaggaga tgagcgaaga agaatttatg tctcgtgccg gacgttttgt tcattaaaat
      541 actcactcgc ctctgctcat tcagaggcga gcaaaattta cttgtcctgc cgctgtgatg
      601 acatcaacga cgtcagaagc cggtcttttt cctgccacaa tgagttcagc cattgctgaa
      661 aatggcgctt aaagcttttg tcattgatgt agtcgccatg taactcatcg gcaataggct
      721 gtaaatccac atgtacgaca atccgcgtca gtttaccgct taacatatcg aagaacggct
      781 gacggttatt gtccggataa cacagcgtaa cattaagcag tttatcgaat tgtttaccca
      841 gtacatttag cgccatcgca atgcctgccg cctttggtgg caacaagttt tgaaaagtgg
      901 agtgggtttg ctgatgtttt tcttgcgtga aacgggagcc ttcaacgaaa ttgacaatgg
      961 tggtgggatg caggcgaaac ttttcacaag aacgccgagt ggtttc
//
LOCUS       DAFATA010000285          988 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFATA010000285 DAFATA010000000
VERSION     DAFATA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.289"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            79..759
                     /locus_tag="LGJ20_004935"
     CDS             79..759
                     /locus_tag="LGJ20_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2388900.1"
                     /translation="MSALQIAERSIANAGRISVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIKKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTTSNAPAARGR
                     RYAQGADV"
BASE COUNT          273 a          235 c          266 g          214 t
ORIGIN      
        1 aagattgctg aaggcgttgc aagccgcgtt attcccggtt ctgacgtccg gcgcggggta
       61 ttgattttct caatggaaat gagcgcattg cagattgcag agcgaagcat tgccaacgcc
      121 gggaggatat cggttagcgt actgcgaaat cctgcatcga tggatgacga gggctgggca
      181 cgtgttgcta acggcatgag tcagcttgca gatttggatg tatgggtagt cgatgcctcg
      241 cggttatcag tcgaagaaat acgctcaatc gcagaacggc acaaacagga aaatccaaac
      301 ctctcactca tcatggcgga ttatcttggc ctgattaaga agccgaaagc agaccgcaac
      361 gacctcgcaa ttgctcacat ctccggaagc ctgaaggcga tggcgaaaga cctgaaaaca
      421 ccggttatct ccctgagtca gctttcgcgc gatgttgaga agcgaccaaa caaacgcccg
      481 acaaacgcag atttgcgtga ttcaggaagc attgagcagg acgcagactc aatcatcatg
      541 ctctatcggg aagcggtata tgacgagaac agtagcgccg cgccatttgc tgaaatcatt
      601 gtgacgaaaa accgttttgg ctcgcttggt acggtttacc agcggttctg caacggacac
      661 tttgttgcat gtgaccagga tgaagccaga cagatttgca caacatcaaa tgcacccgct
      721 gcacgtggca gacgatatgc acaaggggct gacgtatgac catctacatc actgagataa
      781 taacaggggc tatttacaca gtagcccttt tttattggat taagaacgag ggggattctg
      841 atggacaccg ttaacggaat gtgttcagac gcaccgcgtg ccaaaaaatg taaatgcgga
      901 aaatcaccga caatattcga catggagaac gggtgccaaa tctactgcgc taaccacgct
      961 gctgtggcgg ccgcgaatta tcgcagtg
//
LOCUS       DAFATA010000286          958 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFATA010000286 DAFATA010000000
VERSION     DAFATA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.290"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..191
                     /locus_tag="LGJ20_004936"
     CDS             <1..191
                     /locus_tag="LGJ20_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CesD/SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HBK2388901.1"
                     /translation="NYFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVK
                     SKAQAYIELLNDNSEDNG"
     gene            525..>958
                     /gene="ygeH"
                     /locus_tag="LGJ20_004937"
     CDS             525..>958
                     /gene="ygeH"
                     /locus_tag="LGJ20_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HBK2388902.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQI"
BASE COUNT          334 a          151 c          161 g          312 t
ORIGIN      
        1 acaattactt cccgtatgat gactattcag ttatttatcg tatgggtcaa tgtcagattg
       61 gggctaaaaa tatcgataac gcaatgcaat gtttctatca cattattaac aattgtgagg
      121 atgatagtgt taagagtaaa gcgcaggcat atattgaact cttaaacgat aattcagaag
      181 ataatggcta agcattacaa tcatttggtt aaatttgctg gttatagtgc agttaaccac
      241 tataaccagc acctcttatg ttttcttaaa tcagcatctt aaaacagtta agagcatact
      301 ataaatcatt atttttcaat gcgttagaga acaatctact ttatcaaaat tgaataaggc
      361 tatttcagat tacatcactg aattcctaag ataaattagt gaacacaacc atagaatgtc
      421 gaatgtaatt tcctgtaacg ttaatgacac ataaatatga atagccataa tttctattgc
      481 ttaagcagga tgcaagaaac caattttttc atagaggtta actaatggac ttagaaaata
      541 aattctcata tcattttctt gagggattaa cgctcacgga agatggaatt cttactcaag
      601 gaaatgagca agtttatatt ccacagaaag agttaggtgt attaatagta ttacttgaat
      661 ccgctggtca tgttgtactg aaagatatga tcatcgaatc agtatggaaa aatattattg
      721 ttagtgacga gtccctgaca agatgtatct attctttgcg ctgcattttt gaaaaaattg
      781 gctatgatcg ttgcatagaa acaatctacc ggaaaggtta tcgtttcagc gggcaggttt
      841 tcaaaactaa aattaatgaa gataatactt cagactattc catagctata ttccctttca
      901 ctacttcgtt gaatacactg gatccattaa tacttaatca ggaattagtg caaatcat
//
LOCUS       DAFATA010000287          954 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFATA010000287 DAFATA010000000
VERSION     DAFATA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.291"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>954
                     /locus_tag="LGJ20_004938"
     CDS             <1..>954
                     /locus_tag="LGJ20_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HBK2388903.1"
                     /translation="ACVINQAAATGGDARQLVGYLVSQSGLPLDTSALQAQLRETLPP
                     HMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAPKAGSETIIAAAFSSLLGC
                     DVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVMVASTVAKLATIIDAEEDS
                     TRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYLDPQWSIIGIQSPRPNGPM
                     QTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQGIAARLRARGEQVAFLGLL
                     DTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGST"
BASE COUNT          194 a          260 c          293 g          207 t
ORIGIN      
        1 acgcctgtgt gattaaccag gcggctgcca ccggtggtga tgcgcgtcaa ttggtgggct
       61 atctggtgtc gcaatcgggc ctgccgttgg ataccagcgc attgcaggcg cagcttcgtg
      121 aaacattgcc accacatatg gtaccggtgg ttctgctgca acttccacag ttaccactta
      181 gcgccaacgg caagctggat cgcaaagcct tgccgttgcc tgaactgaag gcacaagcgc
      241 cagggcgtgc gccgaaagcg ggcagtgaaa cgattatcgc cgcggcattc tcgtcgttgc
      301 tggggtgtga cgtgcaggat gccgatgctg atttcttcgc gcttggcggt cattcgctac
      361 tggcaatgaa actggcagcg cagttaagtc ggcaggttgc ccgccaggtg acgccggggc
      421 aagtgatggt cgcgtcaact gtcgccaaac tggcaacgat tattgatgct gaagaagaca
      481 gcacccggcg tatgggattc gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc
      541 tgttttgttt ccatcctgcg tccggttttg cctggcagtt cagcgtgctc tcgcgttatc
      601 tcgatccaca atggtcgatt atcggcattc agtcaccgcg ccccaatggc cccatgcaga
      661 cggcggcaaa cctggatgaa gtctgcgaag cgcatctggc aacgttactt gaacaacaac
      721 cgcatggccc ttattacctg ctggggtatt cccttggcgg tacgctggcg cagggtattg
      781 cggcgcgact gcgtgcccgt ggcgaacagg tggcatttct tggcttgctg gatacctggc
      841 cgccagaaac gcaaaactgg caggaaaaag aagctaatgg tctggacccg gaagtgctgg
      901 cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt actt
//
LOCUS       DAFATA010000288          948 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFATA010000288 DAFATA010000000
VERSION     DAFATA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.292"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            19..780
                     /gene="ydeO"
                     /locus_tag="LGJ20_004939"
     CDS             19..780
                     /gene="ydeO"
                     /locus_tag="LGJ20_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBK2388904.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYT
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          288 a          184 c          184 g          292 t
ORIGIN      
        1 ttgttttaag gagataaaat gtcgctcgtt tgttctgtta tatttattca tcatgccttc
       61 aacgctaaca ttttagataa agattacgcc ttctctgacg gcgagatcct gatggtagat
      121 aacgctgttc gtacgcattt tgaaccttat gagcggcatt ttaaagaaat cggatttaat
      181 gaaaatacca ttaaaaaata tctacaatgc actaacatcc agacagtgac ggtgcctgtt
      241 cctgcgaagt ttttacgtgc ttcaaatgta ccgactggat tgcttaatga aatgattgct
      301 tatctcaact cggaagaacg caatcatcat aatttttcag aacttttgct tttttcttgc
      361 ctgtctatat ttgccgcatg caaaggtttc attacactat taactaacgg tgtgctatcc
      421 gtttccggga aagtgagaaa tattgtcaac atgaagctgg cgcacccatg gaagctgaaa
      481 gatatttgtg actgcctgta catcagtgaa agcctgttga agaaaaaact aaagcaagag
      541 caaacgacat tctcacagat tctattagat gccagaatgc agcacgcaaa aaatttgata
      601 cgcgtagaag gttcagtcaa taaaattgcc gaacaatgtg gttatgccag tacatcttat
      661 tttatttata cgttccgcaa acatttcggc aacagtccga agagagtttc taaggagtac
      721 cgttgtcaaa gtcacacggg tatgaatacg ggcaacacga tgagtgcttt agctatttga
      781 ttatttgcta acgagtagtc aaccacgcac gctacgtaag aattatatgg ggcagccatt
      841 ccctgccccg cgttgttttt aggcgatata tttattgaaa taaataagtg acatccatca
      901 catatttatg cacttgcata acctgttgca tgattattta tgatctca
//
LOCUS       DAFATA010000289          947 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.293, whole genome shotgun sequence.
ACCESSION   DAFATA010000289 DAFATA010000000
VERSION     DAFATA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.293"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            209..718
                     /locus_tag="LGJ20_004940"
     CDS             209..718
                     /locus_tag="LGJ20_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBK2388905.1"
                     /translation="MNQQRFDDSTLIRIFALHELYRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDYYNQYRQRVIVLLSHPANTRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            715..>947
                     /locus_tag="LGJ20_004941"
     CDS             715..>947
                     /locus_tag="LGJ20_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBK2388906.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREV"
BASE COUNT          219 a          251 c          213 g          264 t
ORIGIN      
        1 aacccgtctt catattgttt gcgatgtccc tgatgagctt attgatttca cgtttgaata
       61 gaaagggcag ctatggttag aaactacctg acgtcagtcc ttgcggggag caggctttcg
      121 taaatttgtc ctgctacaac aggattaact tcacaaatat catttctcaa cgtctacact
      181 tactcctgta aaccgttcag gagcagtaat gaatcagcaa cgatttgatg acagcaccct
      241 aatccgtatc ttcgcgcttc atgagttata tcgactgaaa gaacatggct taacgcgcgg
      301 ggcgcttctc gattatcaca gccgctataa actcgtcttt ctggcgcatt ctcagccgga
      361 ataccgcaaa cttggcccgt ttgtggctga tattcatcgg tggcaaaatc tggatgacta
      421 ttacaaccag taccgccaac gcgtaatcgt tttactttct caccccgcca acacgcgcga
      481 tcacaccaac gttttgatgc acgttcaggg ttattttcgc ccacatattg attccacaga
      541 acgccagcag ctggctgcgc ttatcgacag ttatcgccgt ggcgagcaac cgcttcttgc
      601 gccgctgatg cgtatcaaac actatatggc gctttatcct gacgcctggc tttcagggca
      661 gcgttatttc gaactttggc cgcgtgtgat taacttgcgc cattcaggag ttttatgact
      721 acccatctgg tctggtttcg ccaggattta cgtctgcatg ataatctcgc gcttgctgcc
      781 gcctgccgca attcgtctgc acgcgtgctg gcgctgtata tcgctacacc acgccagtgg
      841 gagacgcata acatgtcgcc gcgtcaggct gaacttatca atgctcaact gaatgggcta
      901 caaatagcgc ttgcggaaaa aggtattcct ttattgttcc gtgaagt
//
LOCUS       DAFATA010000290          897 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFATA010000290 DAFATA010000000
VERSION     DAFATA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.295"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..216)
                     /locus_tag="LGJ20_004942"
     CDS             complement(<1..216)
                     /locus_tag="LGJ20_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388907.1"
                     /translation="MNEQTPNPNATNEGINEQAAVSSLPVSPESKPEVVTEVQPEVQK
                     ETDSQAADKRKQVLDEAVSALSLTKSAL"
     gene            complement(319..>897)
                     /gene="yfdX1"
                     /locus_tag="LGJ20_004943"
     CDS             complement(319..>897)
                     /gene="yfdX1"
                     /locus_tag="LGJ20_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein YfdX1"
                     /protein_id="HBK2388908.1"
                     /translation="LALAPVDVRVITHDIHANVESVKKAVKLSRELLGDGEVQKARPI
                     VANLASEIVIQTDNLPMATYPAAIKSAARLIDSGKIDNAKAELARALNTLVVTSVAFP
                     LPVLRAEAAMAKAEKLAETDRRDAKQNEELSTLLSSVRTEIEMAQILGYGKKADFKPI
                     FDQVKSIEQKSAGGKSGKGWFDELKTRIQKLF"
BASE COUNT          161 a          258 c          247 g          231 t
ORIGIN      
        1 cagcgctgat ttggtcagcg acaaggccga gactgcctca tcgaggactt gtttacgttt
       61 gtctgccgcc tgcgagtccg tttctttctg aacctcaggc tgtacctccg tgacgacttc
      121 aggcttggac tctggactga caggaaggct gcttaccgcg gcttgttcgt ttattccttc
      181 gttcgtcgca ttgggattcg gtgtttgctc attcatggtg atatgcctcg tatgagatag
      241 tgaaaatagg cgaatctgtt ggggctgaat gcgaacatcg cgagactgac cgacagagca
      301 gtcagcccct tcatcacctc aaaacagctt ttggatgcgc gtcttcaact cgtcgaacca
      361 tcccttgccg cttttgccac cagccgactt ttgctcaatg gacttcacct gatcgaaaat
      421 gggtttgaag tccgccttct tgccataacc caggatctgc gccatctcga tctccgtgcg
      481 cacggacgac agcaaggtgc tgagctcctc gttctgcttg gcatcgcgcc tgtcggtctc
      541 ggccagcttt tcagcttttg ccatcgcggc ttcggcccgt agcacgggca gaggaaaggc
      601 gaccgaggtc accaccagcg tgttcagtgc tcgggcgagt tccgctttgg cgttgtcgat
      661 cttgccgctg tcgatgagcc gtgcggccga cttgatcgct gccgggtacg ttgccatcgg
      721 aaggttgtcg gtttggatta cgatttcgct ggccagattg gcaacgatgg gccgggcctt
      781 ttgcacctcg ccatcaccca acaactcccg agacaacttg accgctttct ttaccgattc
      841 cacgttggcg tggatatcgt gggtgatgac gcgtacatcg accggcgcca aagcaag
//
LOCUS       DAFATA010000291          893 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFATA010000291 DAFATA010000000
VERSION     DAFATA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.296"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            173..>893
                     /locus_tag="LGJ20_004944"
     CDS             173..>893
                     /locus_tag="LGJ20_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HBK2388909.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYE"
BASE COUNT          220 a          226 c          244 g          203 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag tgtcagtcat
      421 taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca agactgatac
      481 cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag cctgggaagc
      541 gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg cgctgacaga
      601 tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat
      661 tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga
      721 cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg
      781 tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc
      841 ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg aat
//
LOCUS       DAFATA010000292          883 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFATA010000292 DAFATA010000000
VERSION     DAFATA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.297"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..573
                     /locus_tag="LGJ20_004945"
     CDS             <1..573
                     /locus_tag="LGJ20_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HBK2388910.1"
                     /translation="VSNQPEHKVEAKDSNGMPVDNRQGTITVSASGLQVGDAFTIAGV
                     NSVHQITKDTTGQLQVFRVLAVDSAGTTVTISPKILPVENTDVASRPYANVDAKPAES
                     AAITILNENAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDH
                     IKGVTTARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            615..803
                     /locus_tag="LGJ20_004946"
     CDS             615..803
                     /locus_tag="LGJ20_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388911.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            784..>883
                     /locus_tag="LGJ20_004947"
     CDS             784..>883
                     /locus_tag="LGJ20_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head DNA stabilization protein"
                     /protein_id="HBK2388912.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIE"
BASE COUNT          244 a          212 c          223 g          204 t
ORIGIN      
        1 gtatcaaatc aacctgaaca caaggttgaa gcgaaagatt caaatggtat gccagttgat
       61 aaccggcagg ggactattac ggtatctgca tctggcttgc aggttggtga tgcgttcacc
      121 attgccggtg tgaattccgt acaccagatc accaaagata ccaccgggca gctgcaggta
      181 ttccgtgttc tggcagtaga ctcagcagga accaccgtaa caatctctcc aaagattctc
      241 cctgttgaaa atactgatgt tgcgagtcgt ccatatgcaa acgtcgatgc caaaccggca
      301 gaatcagcag caatcaccat tctcaacgag aacgcagcac cggctaacct attctgggct
      361 gatggttctg ttgagctgat gtacggcaaa ctggcgttcc cgactggtca gggtccacag
      421 gtaatgacag caaccaccga gcagggcgct acgctgatca tgtcttacgc cttcgaccac
      481 atcaaaggtg taaccactgc gcgtttcacc actctgtacg gttgctctgt actggttcct
      541 gaatatacgg gcatcgttat tgccgggcag taattttggt ggggcttcgg ccccattttt
      601 attgggagaa gacaatggca cgaacaatgc tctataagcc gggcaacatg atcacctgcg
      661 gtcagtttgc tgtcgattac atcattgttg atgacgaaga agttaaatct cacctgaaaa
      721 aaggttgggt aaaaactcct gaagaaaccg caacgaagca aaaagtggct aaggcggaag
      781 aagatggcga aaacgaaggg tgatctcgtt ctaaaggctt tacgaaaagc cgggctgtat
      841 tccaatgcca cgttgacaga tgctgaccct caggcaattg aag
//
LOCUS       DAFATA010000293          873 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFATA010000293 DAFATA010000000
VERSION     DAFATA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.298"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>873
                     /locus_tag="LGJ20_004948"
     CDS             <1..>873
                     /locus_tag="LGJ20_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2388913.1"
                     /translation="YGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCI
                     PDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQL
                     QSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPY
                     ATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRYGRLTEKTDLIPEGGIRT
                     DDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMS
                     LSRKPQVTWYGWDG"
BASE COUNT          197 a          285 c          261 g          130 t
ORIGIN      
        1 attacgggta tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catcacccgc
       61 agacggaagc actgctctgg cagcatgaga ccagacatgc gtacaacgcg caggggctgg
      121 cgaaccgctg tataccggac agcctgcccg ccgtggaatg gctgacctac ggcagcggtt
      181 acctggcagg catgaaactc ggcgacacac cgctggtgga gtacacccgc gaccgcctgc
      241 accgggaaac gctgcgcagc ttcggccgtt atgaactcac caccgcttat acccctgccg
      301 ggcagttaca gagccagcac ctgaacagcc tgctgtctga ccgcgattac acctggaacg
      361 acaacggcga actcatccgc atcagcagcc cgcgccagac ccggagttac agctacagca
      421 ccaccggcag gctgaccggc gttcacacca ccgcagcgaa ccttgatatc cgcatcccgt
      481 atgccacaga cccggcaggt aaccgcctgc ccgacccgga gctgcacccg gacagcaccc
      541 tcagcatgtg gccggataac cgtatcgccc gtgacgcgca ctatctttac cggtatgacc
      601 gttacggcag gctgacggag aaaaccgacc tcatcccgga agggggtatc cgtacggatg
      661 atgagcgcac ccaccggtac cattacgaca gtcagcaccg gctggtgcac tacacgcgga
      721 cacaatatgc agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg
      781 tggcaaaacg ggtatggcgg cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga
      841 aaccgcaagt gacctggtac ggctgggacg gcg
//
LOCUS       DAFATA010000294          863 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFATA010000294 DAFATA010000000
VERSION     DAFATA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.299"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..659)
                     /gene="cas1e"
                     /locus_tag="LGJ20_004949"
     CDS             complement(<1..659)
                     /gene="cas1e"
                     /locus_tag="LGJ20_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HBK2388914.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IA"
     gene            complement(675..>863)
                     /gene="cas6e"
                     /locus_tag="LGJ20_004950"
     CDS             complement(675..>863)
                     /gene="cas6e"
                     /locus_tag="LGJ20_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBK2388915.1"
                     /translation="DVHPISERPQYFSGDGKSGKIQTVCFEGVITINDAPALIDLVQQ
                     GIGPAKSMGCGLLSLAPL"
BASE COUNT          225 a          237 c          199 g          202 t
ORIGIN      
        1 gcaatatcgt aaacaaagga aagaggcttt cctgtatgca caaacccaat agcgggtgca
       61 taaccagctg caagtatcgc cgcttcagtt acgccgtata aacaggaagt tgcagcgcta
      121 atgcattggt tgaccgtatc gcccttttcc cagtctttcg gatcgtagcg acgtccattc
      181 catgtcacgc cgtattgctt cgccagaagt gcgtaggttg cccgcacgcg actgccttct
      241 atacctcgga gttgttctac ggagcgtcgg gcgggcgctg gttcgccaaa ccgtagttca
      301 aacattttgc gtaccacttt cagacgcaaa tcttcatcca gagcaagttt tgcctgatag
      361 agcagcttat ctgaacgcgc accaccaggc tgaccagaag cataaacacg aacacccgct
      421 tcccccaccc ataccaataa tgttccaact tgcgcagcca agcgtacagc tgcatgcgaa
      481 acccgtgtac caggctccag catgatgcag gcaaccgagc caacaggaat atgagtgcgg
      541 atccctgtct tgtcgataag tacaaacgcg ccatcgatta catcgatctg cccatattgc
      601 agaaagatca tggagacgcg atctttgagt ggaatggggt taagaggaag ccaggtcatt
      661 ttattacacc ttaatcacag tggagccagg gatagcaagc cacatcccat cgatttggct
      721 ggcccaatac cttgctgtac aagatctatt aacgctggcg cgtcgttgat ggtgatcacc
      781 ccttcaaagc aaaccgtttg gatctttcca cttttaccat caccagaaaa atactgtggt
      841 cgttccgata tgggatgcac atc
//
LOCUS       DAFATA010000295          863 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFATA010000295 DAFATA010000000
VERSION     DAFATA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.300"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            1..495
                     /locus_tag="LGJ20_004951"
                     /pseudo
     CDS             1..495
                     /locus_tag="LGJ20_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(499..>863)
                     /gene="tcyP"
                     /locus_tag="LGJ20_004952"
     CDS             complement(499..>863)
                     /gene="tcyP"
                     /locus_tag="LGJ20_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBK2388916.1"
                     /translation="NGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVA
                     GVGGGATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLK
                     QTDKAILDSEDDAELAHR"
BASE COUNT          248 a          196 c          192 g          227 t
ORIGIN      
        1 tattaatgag aattttgttg ggatggattt tacctgtgaa ttctgacccg aaaaaatgac
       61 gaatttgatc aatataaaaa atagtttggt tattcattat ttaaggacta tatactatga
      121 aaaaaattat atacatcctg ccgggagcat ttttgctgcg attcaaaaaa aaatgagagt
      181 ctcctaaagt ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta
      241 aatcttccgg gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca
      301 cttagaaaac gtctcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc
      361 caggaatgta ttgataagtt ggacattttt ttcgctgacg atctgtttaa atatgagtca
      421 gtatgcatcg ccgcgatgga acatttaaaa agtaaagcaa cggcccctat acaaaacgga
      481 ccgttgccag cataagaatt aacggtgtgc cagttcggcg tcgtcttcgc tatccagaat
      541 ggctttatcg gtttgcttca gccactggct ggtcagcgtg ccagctgtca tcgagccact
      601 aacgtttagc gccgtacggc ccatgtcgat aagcggttca acggagatta acagcgccac
      661 cagggttact ggcaggccca tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc
      721 accgacaccg gcaacgcctg cggaactaac ggtaacaata ccgaccagcg tcgcaatcca
      781 catcgggtcc agcgggttaa tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg
      841 atacaaaccg gcgcagccgt tct
//
LOCUS       DAFATA010000296          848 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFATA010000296 DAFATA010000000
VERSION     DAFATA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.301"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>848
                     /locus_tag="LGJ20_004953"
     CDS             <1..>848
                     /locus_tag="LGJ20_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK2388917.1"
                     /translation="ALVAGAKYRGEFEERLKGVLNDLAKQEGNVILFIDELHTMVGAG
                     KADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQKVFVAEPSVED
                     TIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRM
                     QIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSDKERQYSELEEE
                     WKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPELEKQLEAATQL
                     EGKTMRL"
BASE COUNT          244 a          201 c          234 g          169 t
ORIGIN      
        1 gcgctggtgg ctggggcgaa atatcgcggt gagtttgaag aacgtttaaa aggcgtgctt
       61 aacgatcttg ccaaacagga aggcaacgtc atcctgttta tcgacgaatt acataccatg
      121 gtcggcgcgg gtaaagccga tggcgcaatg gacgccggaa acatgctgaa accggcgctg
      181 gcgcgtggtg aattgcactg cgtaggtgcc acgacgcttg acgaatatcg ccagtacatt
      241 gaaaaagatg ctgcgctgga acgtcgtttc cagaaagtgt ttgttgccga gccttccgtt
      301 gaagatacca ttgcgattct gcgtggcctg aaagaacgtt acgaattgca ccaccatgtg
      361 caaattactg acccggcaat tgttgcagcg gcgacgttgt ctcatcgcta cattgctgac
      421 cgtcagctgc cggataaagc catcgacctg atcgatgaag cagcatccag cattcgtatg
      481 cagattgact caaaaccaga agaactcgac cgactcgatc gtcgtatcat ccagctcaaa
      541 ctggaacaac aggcgttaat gaaagagtct gatgaagcca gtaaaaaacg cctggatatg
      601 ctcaacgaag aactgagcga caaagaacgt cagtactccg agttagaaga agagtggaaa
      661 gcagagaagg catcgctttc tggtacgcag accattaaag cggaactgga acaggcgaaa
      721 atcgctattg aacaggctcg ccgtgtgggg gacctggcgc ggatgtctga actgcaatac
      781 ggcaaaatcc cggaactgga aaagcaactg gaagccgcaa cgcagctcga aggcaaaact
      841 atgcgtct
//
LOCUS       DAFATA010000297          833 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFATA010000297 DAFATA010000000
VERSION     DAFATA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.302"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>833
                     /gene="tuf"
                     /locus_tag="LGJ20_004954"
     CDS             <1..>833
                     /gene="tuf"
                     /locus_tag="LGJ20_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK2388918.1"
                     /translation="VVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLE
                     LVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA
                     IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRK
                     LLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTP
                     FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREG
                     GR"
BASE COUNT          193 a          205 c          240 g          195 t
ORIGIN      
        1 gtagttgctg cgactgacgg cccgatgccg cagactcgtg agcacatcct gctgggtcgt
       61 caggtaggcg ttccgtacat catcgtgttc ctgaacaaat gcgacatggt tgatgacgaa
      121 gagctgctgg aactggttga aatggaagtt cgtgaacttc tgtctcagta cgacttcccg
      181 ggcgacgaca ctccgatcgt tcgtggttct gctctgaaag cgctggaagg cgacgcagag
      241 tgggaagcga aaatcctgga actggctggc ttcctggatt cttatattcc ggaaccagag
      301 cgtgcgattg acaagccgtt cctgctgccg atcgaagacg tattctccat ctccggtcgt
      361 ggtaccgttg ttaccggtcg tgtagaacgc ggtatcatca aagttggtga agaagttgaa
      421 atcgttggta tcaaagagac tcagaagtct acctgtactg gcgttgaaat gttccgcaaa
      481 ctgctggacg aaggccgtgc tggtgagaac gtaggtgttc tgctgcgtgg tatcaaacgt
      541 gaagaaatcg aacgtggtca ggtactggct aagccgggca ccatcaagcc gcacaccaag
      601 ttcgaatctg aagtgtacat tctgtccaaa gatgaaggcg gccgtcatac tccgttcttc
      661 aaaggctacc gtccgcagtt ctacttccgt actactgacg tgactggtac catcgaactg
      721 ccggaaggcg tagagatggt aatgccgggc gacaacatca aaatggttgt taccctgatc
      781 cacccgatcg cgatggacga cggtctgcgt ttcgcaatcc gtgaaggcgg ccg
//
LOCUS       DAFATA010000298          831 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFATA010000298 DAFATA010000000
VERSION     DAFATA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.303"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..345)
                     /locus_tag="LGJ20_004955"
     CDS             complement(<1..345)
                     /locus_tag="LGJ20_004955"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2388919.1"
                     /translation="MLDMRLGNSGDIPFIMNLVRDGVISGAFFYAEGNGLETVRSELQ
                     SVTQQYRDNIPVGDYLFVFAEKLTRQPVGFSLLTGRNDFAGRASHLEIRMFAVAADEQ
                     GKGIGKSMLASII"
     gene            complement(358..768)
                     /locus_tag="LGJ20_004956"
     CDS             complement(358..768)
                     /locus_tag="LGJ20_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBK2388920.1"
                     /translation="MNVIGSAKDNIRMMAYSFTAPDIMKALIAAKRRGVDVKIVVDEN
                     GNTGRASRAAMNLVTNAGIPLRVNSNYKIQHDKVIIVDGRHVETGSFNYTASAEKYNS
                     ENAIVMWDAPELAGQYLKHWQSRWNQGRDFTPSY"
BASE COUNT          204 a          230 c          190 g          207 t
ORIGIN      
        1 gatgattgag gcaagcatac tcttgccaat gcctttaccc tgctcatcag ccgcaacagc
       61 gaacatacgg atctcaagat gagatgcccg gcccgcaaaa tcattgcggc cggttaacag
      121 cgaaaatccc accggctgcc gcgttaactt ctcggcaaag acaaagagat aatcccctac
      181 ggggatatta tctctgtatt gttgggtgac actttgtagc tcagatctca ctgtttcaag
      241 accgttgccc tcggcataga aaaatgcgcc agatattacc ccatcgcgca ccaggttcat
      301 gatgaaagga atatcgccgc tgtttcctaa tcgcatatca agcatatcgt tctccattca
      361 ataagacggg gtaaaatccc ggccctgatt ccagcgtgat tgccagtgct tcaggtactg
      421 cccggccagt tcgggcgcgt cccacatcac aatcgcgttt tcagagttat atttctcggc
      481 actggccgtg tagttaaaac tgccagtttc cacatgacgg ccatcaacga tgatcacctt
      541 gtcatgctgg attttgtagt tactgttaac ccgcagcgga atacccgcgt tcgtaacaag
      601 attcattgcc gcacgacttg cgcggcccgt attgccattc tcatcaacga caattttcac
      661 gtctacaccg cggcgtttgg ccgcaatcag cgctttcata atatcggggg cagtaaagct
      721 gtaggccatc attctgatgt tatcttttgc cgagccgata acgttgataa cgagctgctg
      781 cgcggttcct tccggcgaga aacctacttg cgccgatgca gcaaaaagag a
//
LOCUS       DAFATA010000299          824 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFATA010000299 DAFATA010000000
VERSION     DAFATA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.304"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..161
                     /locus_tag="LGJ20_004957"
     CDS             <1..161
                     /locus_tag="LGJ20_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HBK2388921.1"
                     /translation="HMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAAD
                     LNTQRGDL"
     gene            158..>824
                     /locus_tag="LGJ20_004958"
     CDS             158..>824
                     /locus_tag="LGJ20_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBK2388922.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLME"
BASE COUNT          149 a          206 c          228 g          241 t
ORIGIN      
        1 tgcatatgct ggttactgaa ctggctgtct ttcgttttat tgacggcaaa atgtggctca
       61 ccgaaattgc cgacgggtgt gatttagcca ccgtgcgtgc caaaacagaa gctcggtttg
      121 aagtcgccgc cgatctgaat acgcaacggg gtgatttatg attggtcgca tatcgcgttt
      181 tatgacgcgt tttgtcagcc ggtggcttcc cgatccactg atctttgcca tgttgctgac
      241 attgctaaca ttcgtgatcg cgctttggtt aacaccacaa acgccgatca gcatggtgaa
      301 aatgtggggt gacggtttct ggaacttgct ggcgtttggt atgcagatgg cgcttatcat
      361 cgttaccggt catgcccttg ccagctctgc tccggtgaaa agtttgctgc gtactgccgc
      421 ctccgccgca aagacgcccg tacagggcgt catgctggtc actttcttcg gttcagtcgc
      481 ttgtgtcatc aactggggat ttggtttggt tgtcggcgca atgtttgccc gtgaagtcgc
      541 ccggcgagtc cccggttctg attatccgtt gctcattgcc tgcgcctaca ttggttttct
      601 cacctggggt ggcggcttct ctggatcaat gcctctgttg gctgcaacac cgggcaaccc
      661 ggttgagcat atcgccgggc tgatcccggt gggcgatact ctgttcagtg gttttaatat
      721 tttcatcact gtggcgttga ttgtggtgat gccatttatc acccgcatga tgatgccaaa
      781 accgtctgac gtggtgagta tcgatccgaa actactcatg gaag
//
LOCUS       DAFATA010000300          818 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.305, whole genome shotgun sequence.
ACCESSION   DAFATA010000300 DAFATA010000000
VERSION     DAFATA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.305"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..590
                     /locus_tag="LGJ20_004959"
     CDS             <1..590
                     /locus_tag="LGJ20_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBK2388923.1"
                     /translation="MTQQIEQLKSQLLKQEQTLASLTGSSGIGNLLNDDISTLKGNLP
                     SDWTQVYSDAMNSSSGITSSAQSMLKEFQSQIDGMNRSEAMSFIKQKLAEKGAYDRVM
                     AQNAYNNEMAELNNIQELTNQIDSTTDMKQIADLQARIQTAQGAIQGEQTKLQLMAML
                     QTSQDKILQEQKQYAQKRFVLGADGEDLSMPNVTE"
     gene            594..>818
                     /locus_tag="LGJ20_004960"
     CDS             594..>818
                     /locus_tag="LGJ20_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HBK2388924.1"
                     /translation="MKKLILPLALFLLAGCDSEHPVSWYKEHDQERKAMVEKCRDDAA
                     LKVKPDCQNALEAEKDLKVFGKNDDGLSMPK"
BASE COUNT          248 a          178 c          210 g          182 t
ORIGIN      
        1 taatgacgca gcaaattgaa cagctcaaat ctcaattgct gaagcaggag caaacgctgg
       61 cttccttaac cggcagttct ggtatcggta atttgctgaa tgacgatatt agtacgctga
      121 aaggcaattt accgtctgac tggacgcagg tatacagcga cgccatgaac agcagttcag
      181 gtatcacttc atctgctcaa tccatgctga aggaatttca gtcgcagatt gacggtatga
      241 accgttccga agcgatgagt ttcattaagc agaagttggc ggagaaaggg gcgtatgacc
      301 gtgtgatggc gcagaacgct tacaacaatg aaatggcgga gctgaataac attcaggagc
      361 tgaccaatca gattgattca accacagaca tgaaacaaat cgctgacctt caggcgcgta
      421 tccagaccgc gcaaggtgct attcaggggg agcaaacgaa gctgcaactg atggcgatgc
      481 tgcaaacctc gcaagacaag attttgcagg aacaaaaaca atacgcccag aagcgctttg
      541 tcctcggcgc tgacggtgaa gatctttcca tgcctaacgt aacggaataa accatgaaaa
      601 aactgatttt acctttagct cttttcctgc tggccggttg tgattctgaa catcctgttt
      661 cctggtacaa ggaacacgat caggagcgta aagcgatggt ggaaaaatgc cgtgatgatg
      721 cggcgctcaa agtgaagccg gattgtcaga acgcgctgga agcagagaaa gatttgaaag
      781 tgtttggcaa aaatgatgat ggcctctcaa tgccaaaa
//
LOCUS       DAFATA010000301          804 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFATA010000301 DAFATA010000000
VERSION     DAFATA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.306"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            258..716
                     /gene="tnpA"
                     /locus_tag="LGJ20_004961"
     CDS             258..716
                     /gene="tnpA"
                     /locus_tag="LGJ20_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK2388925.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          243 a          161 c          224 g          176 t
ORIGIN      
        1 ctgaagaaca ggagcgagaa gggctggacg tcaacagcca cggcgagaat gcctataacg
       61 cgtaacaagc actgcaaaaa acagccggac ggttttcacc tccggctatt ttttgtgcac
      121 agaaaacccc cagctaggct gggggttccg gaaagctttc agctttgagc cagttattaa
      181 aacccctttt gatttgttaa aacaccttgc ggtctggcaa ctgcaagtgt caaacaagaa
      241 atcaaaaggg ggtcccaatg gggaacgaaa agagcttagc gcacacccga tggaactgta
      301 aatatcacat agtttttgcg ccaaaatacc gaagacaggt gttctacaga gagaagcgta
      361 gagcaatagg cagtattttg agaaagctgt gtgagtggaa aagcgtacgg attctggaag
      421 ctgaatgctg tgcagatcat atccatatgc ttgtggagat cccgcccaaa atgagcgtat
      481 ccgggtttat gggatatctg aaagggaaaa gcagtctgat gctttacgag cagtttggtg
      541 atttgaaatt caaatacagg aacagggagt tctggtgcag agggtactac gtcgatacag
      601 tgggtaagaa cacggcgaag atacaggatt acataaagca ccagcttgaa gaggataaaa
      661 tgggagagca gttatcgatt ccctatccgg gcagcccgtt tacgggccgt aagtaacgaa
      721 gttggatgca aatgtcagat cgtgtgcgcc tgttagggcg cggctggtaa gagagcctta
      781 taggcgcatt tgaaaaacct ccgg
//
LOCUS       DAFATA010000302          786 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.307, whole genome shotgun sequence.
ACCESSION   DAFATA010000302 DAFATA010000000
VERSION     DAFATA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.307"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..770)
                     /locus_tag="LGJ20_004962"
     CDS             complement(<1..770)
                     /locus_tag="LGJ20_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388926.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMREIGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     QARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAG"
BASE COUNT          132 a          245 c          213 g          196 t
ORIGIN      
        1 aagccagcca ttgcaagaaa tgcctgccca ctggcggcat cgcctgcggt gaactgcgtt
       61 tcagagccaa gttttaacgc ctgctcacgc agcgccttaa actgcgggct gtttttgtcg
      121 attcgcgtca gtgcctgaac gcgggacatc tctttcccga acccgatcgc aggctgcaaa
      181 aaacgcccgg cagcatagcc gcccgccgct gccgcaccaa ttgccagcgc accacctgtt
      241 ttcagttttc ccgctgtttc ctgcgcgcgc gaataccgct cacgcgcctg cgttacacgc
      301 gcaagcgcct gccgttcgcg ttcaagctgg ttgttgtact gttcggtgcg tctgatggcc
      361 tgctggatgg tgttatcgct gcctgtcagg gaaatgccgt ggcgtttcag ctctccgcca
      421 atctcccgca ttttctgaat ttcccgtgtg cgcgattcat tcaggcgttc aagccgggtg
      481 cttaactgct gcatcagctt ttgttgtttt tcgctgagca ctgtacccgt gcgttgtaac
      541 tgattaaggg cgttaagctg gcgtcgtgct ttcacgatac ccgcatccgc tttactgaca
      601 gcgtcgcggg cgcgctcaaa tgaacgcgcc tgacgctcga gatttttgat cgccccctgc
      661 gttcgctgga tggagtcacc aaactgcccc atcaggcggc gggcgttttc ggcaggccgg
      721 gtcagcctgt caacggcgct gaaagcgacc cggatatcaa gagtcttcat tgtctgcatt
      781 cccgct
//
LOCUS       DAFATA010000303          786 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.308, whole genome shotgun sequence.
ACCESSION   DAFATA010000303 DAFATA010000000
VERSION     DAFATA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.308"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..770)
                     /locus_tag="LGJ20_004963"
     CDS             complement(<1..770)
                     /locus_tag="LGJ20_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388927.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAG"
BASE COUNT          131 a          244 c          216 g          195 t
ORIGIN      
        1 aagccagcca ttgcaagaaa tgcctgtcca ctggcggcat cgcctgcggt gaactgcgtt
       61 tcagagccaa gttttaacgc ctgctcacgc agcgccttaa actgcgggct gttctggtcg
      121 attcgcgtca gtgcctgaac gcgggacatc tctttgccga acccgatcgc aggctgcaaa
      181 aaacgcccgg cagcatagcc gcccgccgct gccgcaccaa ttgccagcgc accacctgtt
      241 ttcagttttc ccgctgtttc ctgcgcgcgc gaataccgct cacgcgcccg tgttacacgc
      301 gcaagcgcct gccgttcgcg ttcaagctgg ttgttgtact gttcggtgcg tctgatggcc
      361 tgctggatgg tgttatcgct gcctgtcagg gaaatgccgt ggcgtttcag ctctccgcca
      421 agctcccgca ttttctgaat ttcccgtgtg cgcgattcat tcaggcgttc aagccgggtg
      481 cttaactgct gcatcagctt ttgttgtttt tcgctgagca ctgtacccgt gcgttgtaac
      541 tgattaaggg cgttaagctg gcgtcgtgct ttcacgatgc ccgcatccgc tttactgaca
      601 gcgtcacggg cgcgctcaaa tgatcgcgcc tgacgctcga gatttttgat cgccccctgc
      661 gttcgctgga tggagtcacc aaactgcccc atcaggcggc gggcgttttc ggcaggccgg
      721 gtcagcctgt caacggcgct gaaagcgacc cggatatcaa gagtcttcat tgtctgcatt
      781 cccgct
//
LOCUS       DAFATA010000304          782 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.309, whole genome shotgun sequence.
ACCESSION   DAFATA010000304 DAFATA010000000
VERSION     DAFATA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.309"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..235
                     /locus_tag="LGJ20_004964"
     CDS             <1..235
                     /locus_tag="LGJ20_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK2388928.1"
                     /translation="IVVVGYAMVLRMMGVKYLMPFFFLGFLAGGYLDLSLLAFGGVGV
                     IMALVYIQLNPQWRKAEPQLQAPASTALDQLDD"
     gene            246..>782
                     /locus_tag="LGJ20_004965"
     CDS             246..>782
                     /locus_tag="LGJ20_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK2388929.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQK"
BASE COUNT          157 a          214 c          220 g          191 t
ORIGIN      
        1 tatcgtggtg gtcggttacg ccatggtgct acgtatgatg ggcgtgaaat atttgatgcc
       61 tttctttttc ctcggtttcc tcgcaggtgg ctacctcgat ctcagtctgc tggcgttcgg
      121 tggcgtcggc gtgatcatgg ccctggtcta catccagcta aatccacagt ggcgtaaagc
      181 tgaacctcag ctccaggccc ccgcttccac cgcccttgac caacttgacg attaacggag
      241 ccatcatgga acagagaaaa attacacgca gcgatctggt gagcatgttt ctgcgctcca
      301 acctgcaaca ggcgtccttt aactttgaac gtattcacgg gctgggcttt tgctacgaca
      361 tgatccccgc catcaagcga ctttacccat taaaagagga tcaggttgcg gcgctcaggc
      421 gacacctggt gttcttcaat accacgccag ccgtatgtgg cccggtcatc ggcgtcactg
      481 ccgccatgga agaggcgcgg gccaacggcg cggaaattga tgacggtacc attaacggca
      541 tcaaagtcgg tctgatggga ccattggcag gagttggcga tccactggtt tggggaacgc
      601 tgcgcccgat taccgctgcg ctcggcgcat ctctggcact ttcgggcaac attctcggcc
      661 cgctgctgtt cttctttatt ttcaacgcgg tgcgtctggc gatgaagtgg tatggcctac
      721 agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat gggcgggaat gtgctgcaaa
      781 aa
//
LOCUS       DAFATA010000305          779 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.310, whole genome shotgun sequence.
ACCESSION   DAFATA010000305 DAFATA010000000
VERSION     DAFATA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.310"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            22..>779
                     /gene="aslA"
                     /locus_tag="LGJ20_004966"
     CDS             22..>779
                     /gene="aslA"
                     /locus_tag="LGJ20_004966"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HBK2388930.1"
                     /translation="MMPVMQHPAQDKETQQKLAELEKKTGKKPNVVIFLLDDVGWMDV
                     GFNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPM
                     YGQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMY
                     TEWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRW
                     MEYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNA"
BASE COUNT          201 a          208 c          207 g          163 t
ORIGIN      
        1 gcgaccacta ttgccgacaa catgatgcca gtaatgcagc atccggcgca ggataaagaa
       61 acccagcaga agctggcaga acttgagaaa aaaaccggta agaagcccaa tgtggttatc
      121 ttcctgctgg acgatgtggg ctggatggac gtcggtttta acggtggcgg cgtggcggtg
      181 ggtaacccta caccagatat cgatgcagtt gccagccagg ggctgatttt aacgtcggcg
      241 tattctcaac caagctcttc cccaacccgc gccaccattc tcaccggaca atactccatc
      301 caccacggca ttctgatgcc gccaatgtac gggcaaccgg gaggtctgca agggttaacc
      361 accctgccgc aattgctgca cgaccaaggt tacgtcaccc aggctatcgg gaaatggcat
      421 atgggggaaa acaaagagtc gcagccgcag aacgttggct ttgatgattt ccgtggcttt
      481 aactcggtgt ctgatatgta caccgaatgg cgcgacgttc acgtcaatcc ggaagtggcc
      541 ctgagtccgg accgttctga atacatcaag caattaccgt tcagcaaaga tgatgttcat
      601 gcagtgcgcg gcggcgaaca acaggccatt gccgacatta cgccgaaata tatggaagat
      661 ctggatcaac gctggatgga atatggcgtt aagttcctcg acaagatggc gaagagcgat
      721 aagcctttct tcctctatta cggcactcgc ggctgtcact tcgataacta cccgaacgc
//
LOCUS       DAFATA010000306          765 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.311, whole genome shotgun sequence.
ACCESSION   DAFATA010000306 DAFATA010000000
VERSION     DAFATA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.311"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            50..>765
                     /gene="rhmD"
                     /locus_tag="LGJ20_004967"
     CDS             50..>765
                     /gene="rhmD"
                     /locus_tag="LGJ20_004967"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HBK2388931.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHA"
BASE COUNT          188 a          172 c          218 g          187 t
ORIGIN      
        1 gatggaaaag tccctttgac ttacaagcct cataacaatg gagaacatca tgaccctacc
       61 aaaaattaaa caggttcgcg cctggtttac tggcggtgcg acagcagaaa aaggcgctgg
      121 cggcggtgat tatcacgacc agggggcgaa tcactggata gacgatcata ttgccacccc
      181 gatgagtaaa taccgcgatt acgagcagtc acgccagtca tttggcatta acgttcttgg
      241 cacgttggtt gttgaagtcg aagcagaaaa cggccagacc ggattcgccg tttcgacagc
      301 cggtgaaatg ggctgtttta ttgtcgaaaa acatcttaac cgtttcattg agggtaaatg
      361 tgtcagtgat atcaaactga tccacgatca aatgctcagt gccaccctgt attactccgg
      421 ctctggtggc ctggtgatga atacgatttc ttgtgtcgat ctggctctgt gggatctgtt
      481 cggcaaagtg gtcgggcttc cggtttataa acttttaggc ggcgctgttc gtgatgagat
      541 tcagttctac gccacaggtg cgcgtccgga tctggcaaaa gagatgggct ttatcggtgg
      601 caaaatgccg acgcactggg ggccacatga tggcgatgcg gggatccgca aagatgccgc
      661 tatggtcgcg gatatgcgtg aaaaatgcgg tgaggatttc tggttaatgc tcgactgctg
      721 gatgagtcag gacgtgaact atgcgaccaa actggcccac gcttg
//
LOCUS       DAFATA010000307          755 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.312, whole genome shotgun sequence.
ACCESSION   DAFATA010000307 DAFATA010000000
VERSION     DAFATA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.312"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>755
                     /locus_tag="LGJ20_004968"
     CDS             <1..>755
                     /locus_tag="LGJ20_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBK2388932.1"
                     /translation="IARELGIYRTTISRLLKRGRDQGIVTIAINYDYNENLWLEQQLK
                     QKFGLKDVVVVSGNDEDEETQLAMMGLHGAQLLDRLLEPGDIVGFSWGRAVSALVENL
                     PQAGQSRQLICVPIIGGPSGKLESRYHVNTLTYSAAAKLKGESHLADFPALLDNPLIR
                     NGIMQSQHFKTISAYWDNLDVALVGIGSPAIRDGANWHAFYGGEESDDLNARQVAGDI
                     CSRFFDIHGAMVETNMSEKTLSIEMNKLKQARYS"
BASE COUNT          189 a          181 c          209 g          176 t
ORIGIN      
        1 atcgcgcgcg aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc
       61 gatcagggaa ttgtcaccat cgccatcaac tatgactaca acgaaaatct ctggctcgag
      121 cagcaactga agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag
      181 gatgaagaga ctcaactggc gatgatgggg ttacatggcg cacaactgct ggatcgcttg
      241 ctggagcctg gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa
      301 aacttgccgc aagcggggca atcgcggcag ttaatctgcg tgccaattat tggcggaccg
      361 tccggtaaac tcgaaagccg ctatcacgta aacacattaa cctacagcgc ggcagcgaag
      421 ctgaaagggg aatcgcatct cgcggatttt ccggctctgc tggataaccc cttaattcgt
      481 aatgggatca tgcagtctca gcactttaaa accatctctg cctactggga taatctggat
      541 gtcgccctgg tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt
      601 tatggtggtg aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg
      661 cgcttttttg atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacactctct
      721 atcgaaatga ataaattaaa gcaggcacga tattc
//
LOCUS       DAFATA010000308          752 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.313, whole genome shotgun sequence.
ACCESSION   DAFATA010000308 DAFATA010000000
VERSION     DAFATA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.313"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            193..543
                     /gene="tnpB"
                     /locus_tag="LGJ20_004969"
     CDS             193..543
                     /gene="tnpB"
                     /locus_tag="LGJ20_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK2388933.1"
                     /translation="MIPLPSGTRIWLVAGVTDMRKSFNGLGELVQHVLDDNPFSGHLF
                     IFRGRKGDTVRILWADADGLCLFTKRLEEGQFVWPAVRDGKIAITRSQLAMLLDKLDW
                     RQPKTARLNSLTML"
     gene            574..>752
                     /locus_tag="LGJ20_004970"
     CDS             574..>752
                     /locus_tag="LGJ20_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063945310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK2388934.1"
                     /translation="MSQDYLARIAALEDALRQKDSQLSLVAETESFLCSALARAEEKI
                     ENEEREIEHLRAQIE"
BASE COUNT          163 a          210 c          208 g          171 t
ORIGIN      
        1 tgttatcccc gttgtcctgc ctgatatggt cctgtcacac catgctgagc cgcactatga
       61 acccgccgct ccagcctgcc gcgaggccat gacatgcgag gtgactgtcg gcggtgccag
      121 cctgcgtctg tccggggatt tatcacctgc acttctgaaa acgctgatcc gcgagctgag
      181 cgggaggagc cgatgatacc cttaccgtca ggcactcgta tctggctggt tgccggggtc
      241 accgatatgc gtaagtcctt caatggtctg ggcgaactgg tccagcatgt tcttgatgac
      301 aatccgttct ccggccacct gtttatcttc cgtggtcgta aaggtgacac cgtgaggatc
      361 ctctgggctg atgctgacgg tctgtgtctg tttaccaaac gtctggaaga gggacagttc
      421 gtctggcctg ctgtacgcga cggcaaaatc gccatcaccc gctcacaact cgccatgctc
      481 ctcgataagc tggactggcg gcaacctaaa actgcacgcc ttaactcact gacgatgttg
      541 taaaaagcgt atgaccgcat tataaatgtg gtcatgagtc aggactatct cgcccgtatc
      601 gctgcgctgg aagacgcgct tcgccagaaa gacagccagc tcagtctcgt tgctgagact
      661 gagtcgttcc tgtgttcggc gctggcccgc gcagaagaga aaatagagaa cgaagagcgt
      721 gaaatagaac atctgcgggc tcagatagaa aa
//
LOCUS       DAFATA010000309          748 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.314, whole genome shotgun sequence.
ACCESSION   DAFATA010000309 DAFATA010000000
VERSION     DAFATA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.314"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>748
                     /locus_tag="LGJ20_004971"
     CDS             <1..>748
                     /locus_tag="LGJ20_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388935.1"
                     /translation="LLWGFLSAAMAWADTEAKFLIVRTLLGAAEAGFFPGMIYLTSQW
                     FPQRNRASIMGLFYMGAPLALTLGSPLSGALLEMHGFMGHPGWFWMFVIEGLLAVGAG
                     VFTFFWLDDTPEQARFLSKQEKTLLINQLASEEQQKVTSRLSDALRNGRVWQLAIIYL
                     TIQVAVYGLIFFLPTQVAALLGTKVGFTASVVTAIPWVAALFGTWLIPRYSDKTGERR
                     NVAALTLLAAGIGIGLFGLLSPVMAIVALCV"
BASE COUNT          142 a          172 c          224 g          210 t
ORIGIN      
        1 ctgctgtggg gatttctttc ggcagccatg gcatgggccg atactgaagc gaaatttctg
       61 atagttcgca ctctgcttgg tgctgcggag gccggatttt tccctggtat gatttatctc
      121 acctcgcaat ggtttccgca gcgtaatcgc gccagcatta tggggctgtt ctatatgggc
      181 gcaccgctgg cgttaacact gggatcaccg ctttccggcg cgctgttgga gatgcatgga
      241 tttatggggc atcccggctg gttctggatg tttgtgattg aaggattgtt ggcagtcggt
      301 gctggggtat tcacattctt ttggcttgat gacacaccgg agcaggcacg ttttctgagt
      361 aaacaagaaa aaacgttgct tatcaatcaa ctggcaagtg aagaacaaca gaaagtgact
      421 tctcggctga gcgatgcgct gcgtaatggc cgagtctggc aactagcgat tatctacctg
      481 accattcagg tggcggttta cggattaatt ttcttcctgc cgacccaggt tgcggcattg
      541 ctgggaacaa aagtgggctt tacagcgtcg gtggtcaccg ccattccgtg ggttgcggcc
      601 ttgtttggga cctggcttat tccgcgctat tccgataaaa ccggcgaacg gcgtaatgtc
      661 gcagcgctga cattactggc ggcgggcatt ggtattggtc tgttcgggct gctttctcca
      721 gtaatggcga tcgtagcgct gtgtgttg
//
LOCUS       DAFATA010000310          743 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.315, whole genome shotgun sequence.
ACCESSION   DAFATA010000310 DAFATA010000000
VERSION     DAFATA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.315"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..301
                     /locus_tag="LGJ20_004972"
     CDS             <1..301
                     /locus_tag="LGJ20_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="regulates the genes involved in 2-keto-3-deoxy
                     gluconate transport and catabolism; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBK2388936.1"
                     /translation="EGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRC
                     IAVPVFDRFGVVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(444..683)
                     /locus_tag="LGJ20_004973"
     CDS             complement(444..683)
                     /locus_tag="LGJ20_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388937.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
BASE COUNT          200 a          163 c          173 g          207 t
ORIGIN      
        1 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
       61 acccgttctg gaccaggtgc gcgagcaggg gtatggcgaa gataacgaag agcaggaaga
      121 agggctgcga tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
      181 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
      241 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
      301 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
      361 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
      421 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
      481 agtgggcaat ctgttatccc caagaaacca cttttagtgt gcaagtattg taccgtgctg
      541 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
      601 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
      661 actgtgcgaa tgatgaatcg cattacaacc tcttctcttt ttatgttcgc ttaatcgtag
      721 cggcaatatg cgctgaagca agc
//
LOCUS       DAFATA010000311          737 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.316, whole genome shotgun sequence.
ACCESSION   DAFATA010000311 DAFATA010000000
VERSION     DAFATA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.316"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
BASE COUNT          145 a          222 c          259 g          111 t
ORIGIN      
        1 ttttagtatc attatatagt acgaaaaaga tacttaaatt ttgcaccgct gccgacaaat
       61 gaaaggcaac tgcgccgatc tccacctggg cggcgtggtc aaattgccgg gccttacggc
      121 atcgagctgg gcgacgcgcc cacttccccg gagccgaacg gggcagggca ttacgtcgcc
      181 ggtgacgccg accagggcga agcgcccacc cccctccctg caaagccggt tttggcgggc
      241 tgtcagacga taccaccgcc ggaacagtgg cccgtcacaa cggcccggtt gagcgggcgg
      301 gctggcgcgt agcgacgggc cgggcgtgat ttatccgggt gttgggggga tggccgcgca
      361 gcggccagca cccttacagg cacgattgcg ccgttgctgg cagaggatgg cgaagccacc
      421 ccgaaggggc ggggccgccg gaggcggggc aagtccggcg cagggcgtgg gggccaagcg
      481 gagcgcggag ggcaaagccc ggaggcgtta gggattgaag cccgaagggg cgagacagct
      541 tgcctggctc gatgcgcagc acgaaagccc ggcccgcagg gaacgccctg agtaaccggc
      601 ggccgctgcc gggccaatca cggcgatcac ccaaaataac cctgctgtga aatccacgga
      661 tcgtacagca gggtaagcca ggggagcagg ggaaaggggc ggcttgccgc ccctttccgt
      721 tacttcttct tgctttt
//
LOCUS       DAFATA010000312          716 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.317, whole genome shotgun sequence.
ACCESSION   DAFATA010000312 DAFATA010000000
VERSION     DAFATA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.317"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>716
                     /gene="alsK"
                     /locus_tag="LGJ20_004974"
     CDS             <1..>716
                     /gene="alsK"
                     /locus_tag="LGJ20_004974"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HBK2388938.1"
                     /translation="TAEVIAPGLVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKR
                     TIISTPNLPLTAADLYDLADKLENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAY
                     LGTGMGFAVWMNGAPWTGAHGVAGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRW
                     YEQQPRNYPLRDLFVHAENAPFVQSLLENAARAIATSINLFDPDAVILGGGVMDMPAF
                     PRETLVAMTQKYLRRPLPHQ"
BASE COUNT          152 a          200 c          203 g          161 t
ORIGIN      
        1 ggaccgcaga agtcattgct cccggcctgg tgtcgggtat cggcgaaatg attgacgagc
       61 aactcaggcg ctttaacgct cgctgtcatg gtctggtgat gggatttccg gcgctggtca
      121 gtaaagataa acgcaccatt atttctacgc ctaacctgcc gttaacagcg gcggatttat
      181 atgatctcgc cgataagctc gaaaatacgc tgaattgtcc ggttgagttt tcccgcgacg
      241 ttaacctgca actctcctgg gacgtagtag aaaaccgcct tacgcaacaa ctggttctgg
      301 cggcctatct cggtacgggg atggggttcg cagtgtggat gaacggtgcg ccgtggacgg
      361 gtgcacacgg tgtggcaggc gaactgggtc atatccccct gggagatatg acccaacact
      421 gcgcgtgtgg caatcctggg tgcctggaaa ccaattgctc tggaatggcg ctaagacgct
      481 ggtacgaaca acagccccga aattacccat tgcgcgatct tttcgtccat gcggaaaacg
      541 cccctttcgt ccagagtctg cttgaaaacg cggcacgggc cattgccacc agcattaatc
      601 tgttcgatcc cgatgcggtg atcctgggcg gtggcgtgat ggatatgccc gccttcccac
      661 gcgagactct cgttgccatg acccaaaagt acctgcgccg tccactgccg catcag
//
LOCUS       DAFATA010000313          703 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.318, whole genome shotgun sequence.
ACCESSION   DAFATA010000313 DAFATA010000000
VERSION     DAFATA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.318"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            10..408
                     /locus_tag="LGJ20_004975"
     CDS             10..408
                     /locus_tag="LGJ20_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBK2388939.1"
                     /translation="MAMNAWAHPDGEKVPAAEITQAYFELGMTFPELYDDSHPEALAR
                     NTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRELVETRERLV
                     RDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            480..>703
                     /locus_tag="LGJ20_004976"
     CDS             480..>703
                     /locus_tag="LGJ20_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HBK2388940.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSIL"
BASE COUNT          189 a          158 c          201 g          155 t
ORIGIN      
        1 cacatccgca tggcgatgaa tgcctgggcg catccggacg gtgaaaaagt tccggcagct
       61 gaaataaccc aggcttattt tgaactgggt atgacgttcc cagaactgta tgacgacagc
      121 catccggaag ccctggctcg caatacccag aaaattttcc gctgggtaga gaaagatacc
      181 cctgatgcag ttgaaaaaat tcaggcgttg ttaccagcga tcgaaaaggc aatgccacct
      241 ttgctggtgg ccagaatgcg cagccacagt tcagcttatt ttcgggagct ggtggagacg
      301 cgggagcgac tggtgagaga cgctgatgat tttgtcgcag tggcaatcgc cggtttcaat
      361 cagatgaacc gtggtggccc ggcaggaaat gctgtggcag tgcattgagt gataatagcc
      421 atatcgaatc acttccggca actcgtgagt aaaaagattc ggtatcagaa gaggtgagta
      481 tggctaacgc ctggctcaga ttatggcatg acatgccaaa tgaccctaag tggcgaacaa
      541 ttgccagggt gtcagggcag ccaattgcaa cagtgatggc agtgtatatc cacctcctgg
      601 tgagcgcgtc acgaaatgtc acgcgaggtc acattgatgt cacgacagaa gatttggcaa
      661 gtgcgctcga cgtgacagaa gaggtaattg attcaatttt gca
//
LOCUS       DAFATA010000314          697 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.320, whole genome shotgun sequence.
ACCESSION   DAFATA010000314 DAFATA010000000
VERSION     DAFATA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.320"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..199)
                     /locus_tag="LGJ20_004977"
     CDS             complement(<1..199)
                     /locus_tag="LGJ20_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2388941.1"
                     /translation="MKKRFSDEQIICILREAEAGVSARELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENARLK"
     gene            354..697
                     /locus_tag="LGJ20_004978"
                     /pseudo
     CDS             354..697
                     /locus_tag="LGJ20_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611504.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          174 a          172 c          195 g          156 t
ORIGIN      
        1 tcttgagtct ggcgttctct tcctcaagcg acttcaggcg cttaacctca ggcacctcca
       61 taccgccata cttcttacgc catgtgtaaa aggtggcgtc ggaaatggcg tgcttacggc
      121 agagctcacg ggcagaaacg ccggcttcgg cctcgcggag aatacatatg atctgttcgt
      181 cggaaaaacg cttcttcatg gggatgtcct catgtggctt atgaagacat tactaacatc
      241 acggtgtatt aatcaacggg gagcaggtca gtgttgatct cctccctgaa aacagtgcca
      301 ggctaaactg aagtctccgg tctttcttcc atctcacaga gaggcacatc gccatgaaaa
      361 agacccgcta taccgaagaa aagattgcgt ttgttctgaa acaggccgaa accggcaccc
      421 gcgccgggga agtctgccgg aagatgggca tttctgaagc cactttttat aactggaaga
      481 agaaattcgc cggtctgggc gtgaccgaac tgcggcgtct gcggcaactg gaggatgaaa
      541 atctgcggct gaagaaactg gtgtctgacc tgaatctgga caaggagatg ctgcaggagg
      601 tactgaaaca aaagttctga ggccggctca gaaacgtcag gcggtgcatt ttctgcggga
      661 ggcttaccgt atcagtgtcc gacgggaatg cggactg
//
LOCUS       DAFATA010000315          695 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.321, whole genome shotgun sequence.
ACCESSION   DAFATA010000315 DAFATA010000000
VERSION     DAFATA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.321"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(137..>695)
                     /locus_tag="LGJ20_004979"
     CDS             complement(137..>695)
                     /locus_tag="LGJ20_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBK2388942.1"
                     /translation="VAAQQQTQQVQQTQKTAAAAERISEQGLYAMRDVQVARLALFHG
                     DPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLNDDQYIVINASVGISESYVATPE
                     KEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQYLMPLKQTRNALADAQKLLDKKQ
                     YYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          156 a          170 c          147 g          222 t
ORIGIN      
        1 gaggaatata aaatgcgagt ttatagcacc gcgagcgcca aataatttta tgaccacaac
       61 ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga gttaaatcct ttacctcaat
      121 atactgcctg tcagcattag ttcacaaaca gtgcttcgct gtcgacgatg attccatctt
      181 ccgcaccttt aagcgccagg tttgcctcgt agtattgctt tttatcaagc agtttctgcg
      241 catcagcaag tgcattacga gtctgtttaa gcggcatcag atattggttt tccattacgc
      301 caacaccggc caggcgaagt tcttccattg cgcccttttt atcccctttc gccatttttt
      361 cattggcaat ttttatcgcg gcttcttttt cgggagttgc tacataactc tcagaaattc
      421 cgacagatgc attaatgaca atatattggt catcattgag attggtcttt ttacctggct
      481 tagcgaactt tgcccattcg gtactgtcat ctgacagcaa agcggaagct tcattggtca
      541 gttcttttgc cttttctgga tcgccatgaa acagagccag gcgcgctacc tgcacgtcac
      601 gcatcgcata taaaccttgt tcagagatac gctctgctgc cgctgccgtt ttctgggttt
      661 gctgaacttg ctgagtttgc tgttgtgccg ctacc
//
LOCUS       DAFATA010000316          690 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.322, whole genome shotgun sequence.
ACCESSION   DAFATA010000316 DAFATA010000000
VERSION     DAFATA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.322"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>690)
                     /gene="kefB-GI"
                     /locus_tag="LGJ20_004980"
     CDS             complement(<1..>690)
                     /gene="kefB-GI"
                     /locus_tag="LGJ20_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat resistance system K+/H+ antiporter KefB-GI"
                     /protein_id="HBK2388943.1"
                     /translation="LLLMWLGQPGQSAALLGTAAAMSSTALVSRQLADQGELTTRHGR
                     SAIAVLVFQDLASVPLLALLAIWARGESPKIEHVLLEVFGVLLLFAAAALASRRLLHG
                     LLGWVARRGHEESFVLVSLCVVVAAAAAAHAVGVSAALGAFLAGMVLGESDFRHHMES
                     HLRPFRDVLSGVFFVTIGLQLDAAQILSAPLAVLAWLVVLVPVKILLNTLALRATRLS
                     ALDAWRTGIAL"
BASE COUNT          140 a          246 c          225 g           79 t
ORIGIN      
        1 ccaacgctat gcccgtgcgc caggcatcga gggcggacag gcgcgtcgcc cgcaaggcca
       61 gcgtattgag caggatcttg accggtacca gcaccaccag ccacgccagc accgccagcg
      121 gtgccgaaag aatctgtgct gcgtccagtt gcaggccgat ggtcacgaag aacacgcccg
      181 acaacacatc gcgaaacggc ctgaggtggc tttccatgtg gtgccggaag tcgctctcgc
      241 ccagcaccat cccggcgagg aaggccccca gggcggcgga tacgccgacc gcgtgcgcag
      301 cggcggcggc agccaccacc acgcacaagg agacgagcac gaacgattcc tcgtggcccc
      361 gccgtgccac ccagcccagc aggccatgca acagacggcg cgaggcgagg gccgctgccg
      421 cgaacagcag cagcacaccg aatacttcga gcagcacatg ctcgatcttc ggcgactcgc
      481 cgcgcgccca gatcgccagc aaggccagca ggggcacgct ggccaggtcc tgaaagacca
      541 gcacggcgat ggcactgcgt ccatggcggg tggtgagctc accttggtcg gccagttgtc
      601 ggctgaccag cgccgtggac gacatggccg ccgcagtgcc gagcagcgcg gcgctctggc
      661 cgggctgccc cagccacatc aatagcagga
//
LOCUS       DAFATA010000317          675 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.324, whole genome shotgun sequence.
ACCESSION   DAFATA010000317 DAFATA010000000
VERSION     DAFATA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.324"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            30..>675
                     /locus_tag="LGJ20_004981"
     CDS             30..>675
                     /locus_tag="LGJ20_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBK2388944.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYASYPLPYVQQVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTQGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAAAQAPTLAGRIAHGATRLLAENAVGSLAANSAKDDAEALA"
BASE COUNT          188 a          165 c          185 g          137 t
ORIGIN      
        1 agtaactttt cttcactatg gggtgattaa tggctaaagc atggaaagat gttatcgcct
       61 ctccacagta tcaggcgtta gcaccagaac aaaaagcgca ggctcaggag caatacttca
      121 atgaagtcgt tgccccgcaa gccggagaaa atgcagagca ggctaagcaa gctttctatg
      181 cttcctatcc attgccatat gtgcagcaag tggagacaca gcaaccagta gcacagcaac
      241 aaccacagca aagtggattt atgtctgatc ttggcgaagc agtaaaagag actggtcgcg
      301 gactggtgca ggctggcgtg aacgtggcaa acatacctgc atcagttgcc gatgctgtaa
      361 caagcgcggc ggcttgggct ggcggtaaac tcggcattgg cgatggtaca tatcaaccag
      421 cgccacgtgt aacaacgcag ggattagagc aggactttgg ccttcagcaa ggcgcgctga
      481 ctccacaaac gacagaggga agggtatttg ctgaagcatt gccttacctc actcctgctg
      541 gcgttgagag agcggcagca caggcaccaa cacttgctgg tcgaattgct catggggcaa
      601 ctcgccttct agcagaaaac gcagttggat cacttgctgc aaatagtgcg aaagatgatg
      661 cggaagcact cgcca
//
LOCUS       DAFATA010000318          670 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.325, whole genome shotgun sequence.
ACCESSION   DAFATA010000318 DAFATA010000000
VERSION     DAFATA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.325"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            27..670
                     /locus_tag="LGJ20_004982"
                     /pseudo
     CDS             27..670
                     /locus_tag="LGJ20_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          196 a          146 c          164 g          164 t
ORIGIN      
        1 gacgatgaaa tggaatagcc cctaatatgt caaagccaaa ataccctttt gaaaagcgcc
       61 ttgaagtcgt gaatcactac ttcacaactg atgatggtta caggatcatc tcggcacgtt
      121 ttggtgtccc ccgaacccag gtcaggacat gggttgccct ctatgaaaaa catggagaaa
      181 aaggtttaat tcccaaacct aaaggcgtta gtgctgatcc agagttgcgt attaaggtcg
      241 tgaaagctgt gatcgagcag cacatgtccc ttaatcaggc tgctgctcac tttatgcttg
      301 ctggtagtgg ttctgtagcc aggtggctga aggtctatga agagcgcgga gaagctggtt
      361 tacgcgcgct caagattggc accaaaagaa acattgcaat atcagttgat ccagaaaaag
      421 cggcatcagc attggagctg tcaaaagacc gacgcattga ggatcttgaa aggcaagttc
      481 gatttcttga aacgcggctt atgtatctaa aaaagctgaa agccttagct catcccacga
      541 aaaagtgaaa gtactcaacg agctaaggca gttttatcct cttgatgagc ttctcagggc
      601 tgcggagata ccgcgcagta cgttttatta tcatctaaag gctctcagca agcctgacaa
      661 gtatgcggac
//
LOCUS       DAFATA010000319          668 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.326, whole genome shotgun sequence.
ACCESSION   DAFATA010000319 DAFATA010000000
VERSION     DAFATA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.326"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>668
                     /locus_tag="LGJ20_004983"
     CDS             <1..>668
                     /locus_tag="LGJ20_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK2388945.1"
                     /translation="TDFWSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRA
                     LARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRE
                     SLASGEIAAVQSTLVADNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSK
                     LKPGQSLTIGAGSSAQRLANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTAN
                     LQPG"
BASE COUNT          174 a          170 c          189 g          135 t
ORIGIN      
        1 acggacttct ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg
       61 gcattgagtg atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat
      121 gaaagccgtg ctctggcgcg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca
      181 gatatggcgg ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc
      241 acgctgggcg caagtggtcc gcaatacgtg atggtgccaa agaagcatgc agatcaactg
      301 cgtgaatctc tcgcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat
      361 acgccgctta acagccgtgt ttacaccgta cgctctggcg acacgctttc aagtatcgct
      421 tcacgtctcg gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag
      481 ctgaagccag gccaaagttt gacgattggt gcaggcagta gcgcacagcg actggcaaac
      541 aacagcgata gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa
      601 cgccacggcg tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa
      661 ccaggcga
//
LOCUS       DAFATA010000320          663 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.328, whole genome shotgun sequence.
ACCESSION   DAFATA010000320 DAFATA010000000
VERSION     DAFATA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.328"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            59..>663
                     /locus_tag="LGJ20_004984"
     CDS             59..>663
                     /locus_tag="LGJ20_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBK2388946.1"
                     /translation="MAGVNIQPARAIQQIDTEISNLKKLGKVADNETISKLQSYHDEL
                     VRNAGPDGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAI
                     GHNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEIVNNMLFSKNKSEIK
                     TLYNSVGRVGRAQMRNGIIGKAMEKSGGSPDQFLRQLNILQ"
BASE COUNT          196 a          147 c          170 g          150 t
ORIGIN      
        1 gaaaacatca ggaattcgtc gtgctgcagg gaaccgtctt gagcaggttc agaatgcaat
       61 ggcgggagtc aatatccagc ctgcgcgagc aattcagcag attgatactg agatatctaa
      121 tctgaagaag cttggtaagg ttgctgataa cgagacaatt tcaaaacttc agtcatatca
      181 tgatgagctt gttcgcaatg ctggccctga tggtccggta aatctggatt tgaagcaatt
      241 aagcgacctg cgcagtcagt tcagaatgga cgtgaagggt gagcgaccag tgttaccaaa
      301 ccgttccgat gctgccattc agcgcgttta caaggcgatg accgacgata tcaatggtgc
      361 cattggtcat aatcttggca acgatactct ccgtaaatat cagcaggcca atgccgtcta
      421 cgctgacgaa gcggcgaaac taaagaatac caggctgaag aatgttctca tgaaaggcga
      481 cctgacaccg gaaattgtca acaacatgct attcagcaag aacaaatcgg aaattaagac
      541 gctgtataac tcagttggtc gtgttggcag ggcgcaaatg cgcaatggca tcattggaaa
      601 ggcgatggag aaatctggcg gatcccctga ccagttcctt cggcagctta acatcctgca
      661 aaa
//
LOCUS       DAFATA010000321          660 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.329, whole genome shotgun sequence.
ACCESSION   DAFATA010000321 DAFATA010000000
VERSION     DAFATA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.329"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>660)
                     /locus_tag="LGJ20_004985"
     CDS             complement(<1..>660)
                     /locus_tag="LGJ20_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388947.1"
                     /translation="SAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKE
                     AAKAKLPESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGE
                     NGPEIVNGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAIVSRA
                     ATVPLVQRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYD
                     A"
BASE COUNT          120 a          226 c          161 g          153 t
ORIGIN      
        1 catgcgtcgt atcccccctg atcgctgtag ttactcctgg cttttgcctt cagcctgcgt
       61 tcacgttcat caagctggcg tgccacctcc cgcgcaatat cctgcgcact ttgtcctggc
      121 tgcgtctgaa tgatgatctg cgtcggtgcc tcaatccgtt gaacgagcgg cacagtggct
      181 gcgcgactca caattgcttc tccacctttc gcgggaagtg ccaaagggtg caacggtgga
      241 agctctgctg gcgcggcagc aacgcccatc attccggcaa caacggcagc cagtgcagct
      301 gtatttctcc ggctggtcac atttgccggg ccgttaacaa tttccggccc gttttcaccg
      361 acgatgccaa actgcccgcg cgggatatac ccgccgctgt catacatccc cgcaaagcca
      421 tatccccatg atggaaaacc acccgatggc atcatcactt taccgtctgc attcaccgtc
      481 gcaggttgct gacgcgtcac gctttccggc agttttgcct ttgcggcctc tttactgaca
      541 atgccgagct tctccagcaa ccaggaaacg ccggatttca gggagtccag cggatgcatg
      601 accatattca gcccttccgc cagtgcctcc ccgaatcgcc gccccattgc cgctgcgctc
//
LOCUS       DAFATA010000322          645 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.330, whole genome shotgun sequence.
ACCESSION   DAFATA010000322 DAFATA010000000
VERSION     DAFATA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.330"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            221..>645
                     /locus_tag="LGJ20_004986"
     CDS             221..>645
                     /locus_tag="LGJ20_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2388948.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGND"
BASE COUNT          181 a          141 c          145 g          178 t
ORIGIN      
        1 catatctcac cttcccctgt gattaacctg atgatattgg acaaaaaaac aacgcaggct
       61 gacaaacgat aaaatttgcc tttcacatga ggtaaattgc atcaatggag tgatttacat
      121 ctaaaaatga aggtgaattg agatatggtt cactttagct catctcatag ctgaaatttt
      181 gccgctattt ttttacactt aagcgaaaaa tgaggtgatt atggaacccc ttcgtgaaat
      241 aagaaatcgg ctgcttaacg gctggcaatt atcaaaaatg catacttttg aagtggctgc
      301 caggcatcag tccttcgccc tggcggcaga ggaattgtcg ctgagcccca gtgcggtaag
      361 tcaccgtatc aatcagctgg aagaagaatt gggcattcag ttgtttgttc gttcccatcg
      421 caaagtggaa ttaacgcacg aggggaaacg tgtttattgg gcgctaaaat cgtcgctgga
      481 taccctgaac caggaaattc ttgatatcaa aaatcaggag ttatcgggaa cgttaactct
      541 gtattcccgg ccctctatcg cccaatgctg gttggtgccc gcattaggtg actttacacg
      601 ccggtatccg tctatttcgc tcaccgtgct cactggtaat gacaa
//
LOCUS       DAFATA010000323          643 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.331, whole genome shotgun sequence.
ACCESSION   DAFATA010000323 DAFATA010000000
VERSION     DAFATA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.331"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..483
                     /locus_tag="LGJ20_004987"
     rRNA            <1..483
                     /locus_tag="LGJ20_004987"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          172 a          149 c          180 g          142 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgttct ttgaagtgct cacacagatt gtctgatgaa aagtaaatag
      541 caaggcgttt acgcgttggg agtgaggctg aagagaataa ggccgttcgc tttctattaa
      601 tgaaagctca ccctacacga aaatatcacg caacgcgtga taa
//
LOCUS       DAFATA010000324          638 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.332, whole genome shotgun sequence.
ACCESSION   DAFATA010000324 DAFATA010000000
VERSION     DAFATA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.332"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>638
                     /locus_tag="LGJ20_004988"
     CDS             <1..>638
                     /locus_tag="LGJ20_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HBK2388949.1"
                     /translation="GFSGADLANLVNEAALAARRRRASAVELQDFTATIERIVAGLEK
                     KSRVLNPKERETVAHHEMGHALVLPETDPVHKISIIPRGIGALGYTLQPPTEDRFLMT
                     RTDLEHKIAVLLGGRAAEKLVFGELSTGAADDLARATDIARDMITRFGMDEGLGYIAF
                     EAQRPRFLDTPELAHGGCRVAESTQARIDQAIRDIVMGVFEHAYRILDINRAV"
BASE COUNT          115 a          215 c          208 g          100 t
ORIGIN      
        1 ggcttttccg gcgcggacct cgccaatctg gtcaacgagg cggcgctggc ggcgaggaga
       61 cgccgtgcgt ccgccgtgga attgcaggac ttcaccgcga ccatcgagcg catcgtggcg
      121 ggcctggaaa agaagagccg cgtgctcaat cccaaggagc gggaaaccgt ggcccatcac
      181 gagatgggcc atgcgctggt gctgcccgaa accgaccccg tacacaagat ctcgatcatc
      241 ccgcgcggca tcggcgcgct gggctacacc ttgcagcccc ccaccgaaga ccgcttcctg
      301 atgacgcgta ccgatctcga gcacaagatc gccgtactgc tgggcgggcg tgccgccgaa
      361 aagctggtgt tcggcgagtt gtctaccggg gcggcggacg atctggcgcg agccaccgac
      421 atcgcccgcg acatgatcac ccgctttggc atggacgagg gcctgggcta catcgccttc
      481 gaggcgcagc ggccccgctt tctcgataca ccggaactgg cccacggcgg ttgccgggtg
      541 gccgaatcga cccaggcgcg catcgatcag gctatccgcg acatcgtgat gggcgtgttc
      601 gagcacgcct accggattct cgacatcaac cgcgcggt
//
LOCUS       DAFATA010000325          633 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.333, whole genome shotgun sequence.
ACCESSION   DAFATA010000325 DAFATA010000000
VERSION     DAFATA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.333"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..469)
                     /locus_tag="LGJ20_004989"
     CDS             complement(<1..469)
                     /locus_tag="LGJ20_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK2388950.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVC"
BASE COUNT          165 a          151 c          160 g          157 t
ORIGIN      
        1 cacaaactat cagcgtcttg ataccgtggt tttgcaaaat cagatgtaag tcggtttgat
       61 aaaacgcgcc tttgccaggc ttatcaatca ccggttcgcc tgccaccggg tacagttcag
      121 gaatgatgtc atgacctgct tcacctctga ccagaatgcg tcccatcggt cctggttcac
      181 caataaaggt ttttccgccg cgcgtcagtt tggctggcgg gcagtcgctt aagtctgcac
      241 gatggccttc acgagtatga ataaccataa ttcctttttg acgtgcggct gccagcacct
      301 ctgtacatgg tgcgatagcc gtgcgtacca gtgaaacatc attgcccagt gcctcaccga
      361 agccccctgc ttcgacaaag tcacgctgca tgtcgatcat gaccagtgca gttgtgcgcg
      421 gatcaaaagg taattcaaag ggttgagcct gaaaaatgga ttgcgtcatg gtaaatctcc
      481 tgtctgatgt ttaaacgatc cagttgttct gagaacgagt attcaggaga aaagcggcac
      541 aaaacaggaa aaaagtttgc cgcacagcaa cgaaattttt gcattcagca ggtggtgtga
      601 tgacgaatgt gaagggagtc aaagagtggc ata
//
LOCUS       DAFATA010000326          633 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.334, whole genome shotgun sequence.
ACCESSION   DAFATA010000326 DAFATA010000000
VERSION     DAFATA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.334"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..579)
                     /locus_tag="LGJ20_004990"
     CDS             complement(<1..579)
                     /locus_tag="LGJ20_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBK2388951.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAVMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLW"
BASE COUNT          170 a          167 c          154 g          142 t
ORIGIN      
        1 ccataaccag agcgctgcca gatagcccaa caaatagtca tctccatcgg gcgttaagcc
       61 tttaccaaag ccgatcagtt gtggcacctg gcgttccagc gcatctggat gctccataac
      121 cgctaatccc tggtgcgtga gactatccgg caggtgcaat gtgctttgaa cgttatgttc
      181 cagacaaaat aaaagccgtt gcgatgccat cgttgggtag tgctggaaaa ccaaagggaa
      241 tgcctcaagt gtcagcattc cccccacaaa tggcgactgc cagagcgttg cgttatcgag
      301 cgccacacaa aatcgaggtg tccgcagtaa gcctggggtt aactgaatgg gatctgaatg
      361 ggcaatctct cttcgccagt cccaacattc aggtagccag actctggcag catcagggag
      421 attcagatac gtttgcgcac acaacagagt gatgaatgtt cccgcgtctg aatacagatt
      481 taccgcatgt ttaaaagcgc tatgcagacg taaatcttgc gtttcattct gcgggaaaag
      541 atatcccaac gacgatgcgc aaagcgtaac ctgtaccatc ggcgttagcc ttccagacct
      601 gcaataatgg ctttggaatc agtaacccaa ccg
//
LOCUS       DAFATA010000327          632 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.335, whole genome shotgun sequence.
ACCESSION   DAFATA010000327 DAFATA010000000
VERSION     DAFATA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.335"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            360..>632
                     /locus_tag="LGJ20_004991"
     CDS             360..>632
                     /locus_tag="LGJ20_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2388952.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLL"
BASE COUNT          189 a          124 c          118 g          201 t
ORIGIN      
        1 aaaaaatcca ttgagaacgc attgagagat tcgattgaca tagttagctc caatttccgt
       61 aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc
      121 tctggtaact ggagataatt tttagcataa gggcataact gctaatgtga tcgtaatcac
      181 agtgtgatat tcgtattatc aaataatttt aaaaagcaga agcgtgatta ctctcacaca
      241 acacctctgc tttccatgtt atttattatt tcaattacta gagacctgag aaaagtaatc
      301 cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata
      361 tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg
      421 ttagtttcga cctggcttat ttacgttata tttatcaaat tcccatggcg aaatttatgg
      481 gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg gccattattc
      541 tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg
      601 cgatgatcat taccggatta ctgggtctgt ta
//
LOCUS       DAFATA010000328          629 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.336, whole genome shotgun sequence.
ACCESSION   DAFATA010000328 DAFATA010000000
VERSION     DAFATA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.336"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>629)
                     /locus_tag="LGJ20_004992"
     CDS             complement(<1..>629)
                     /locus_tag="LGJ20_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBK2388953.1"
                     /translation="IKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGLPGT
                     HAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAAQEH
                     HQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTPIAL
                     IGSANLNSRYSQAFNHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNAR"
BASE COUNT          144 a          150 c          177 g          158 t
ORIGIN      
        1 ccctggcgtt ggtggacagt aacccggcag cgtgggcgat tcgccataat ccctgttcag
       61 tggcattggg aatggcgtgt atctgtctgt cgggaaaaac gtggttgaat gcttgtgaat
      121 agcggctgtt cagatttgcg ctaccgatta aggcgatcgg tgttgtggcg ggtaaatcac
      181 gtagcaaact ggatgccaga ccacataatt catggcctat cagtaatccg gaaaggtaat
      241 ccccctgcga ggacgacgta agattactgg tcagcaggcg cgaacgggtc gaaaacagcg
      301 tcgataataa tccggtctga tgatgctctt gtgctgctgt cagcccgtgg gtgaaggcat
      361 cccagtgcgg ttcatctcca ggatgcatgg tggcaccgag aatcgaatgg cgggataaaa
      421 catcaaataa ctcgcccgtc ataaaggttc ggaaatcggt aatggtgttg ttttcaacta
      481 ctgcccattt cgcatgtgtg ccgggtaagc ctatcagcac tggcataccc tgatggatcg
      541 cgttttgcag ggccggttcc atcgagatag caccgaagat ttgtgtctct tcgccacgca
      601 tcacttcggg catttcacct tctttaatc
//
LOCUS       DAFATA010000329          626 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.337, whole genome shotgun sequence.
ACCESSION   DAFATA010000329 DAFATA010000000
VERSION     DAFATA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.337"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>626)
                     /locus_tag="LGJ20_004993"
     CDS             complement(<1..>626)
                     /locus_tag="LGJ20_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBK2388954.1"
                     /translation="RRLSANIDIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQ
                     ALRETRTLNDLIIENAADGVIAIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDN
                     TQFYSPVLDTLEHGTEHVALEISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLT
                     ARKETQRRMAQAERLATLGELMAGVAHEVRNPLTAIRGYVQILRQQTS"
BASE COUNT          144 a          152 c          169 g          161 t
ORIGIN      
        1 cactggtttg ttggcgcaag atctgtacat aaccacgaat agccgttaac ggattacgta
       61 cttcatgcgc gacgccagcc atcagctcac ccagtgtggc gaggcgttct gcttgcgcca
      121 tgcggcgctg ggtttctttg cgggcagtta aatcagagaa aatcaccaaa gcacctatca
      181 tttcaccgtg cgtgttatga atacgactgg tagtgacact gagttcaatg gtgcggtcac
      241 gacctggaaa actgatctcc agcgccacat gttcggtgcc atgttccagc gtatccagta
      301 ctggactgta gaactgagta ttgtcgaaca acatggagta aggctgccct accagttcat
      361 ggcgttgata gccagtgata acttctgctg ccgggttcat ggtggttaca tcaccctggc
      421 ggtcaatggc aatgacgcca tcggcagcgt tttcaataat cagatcgtta agtgtccgcg
      481 tttcacgcag tgcctgggcg aggttattaa cactctgact gatttgcccc atttcaccgg
      541 gcaattgtgg taatcgagtg ggaatatttt gtgccagagt cgagaggcca tcggtgatga
      601 tatcaatatt ggcgctcagg cgacgg
//
LOCUS       DAFATA010000330          625 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.338, whole genome shotgun sequence.
ACCESSION   DAFATA010000330 DAFATA010000000
VERSION     DAFATA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.338"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>625
                     /gene="murD"
                     /locus_tag="LGJ20_004994"
     CDS             <1..>625
                     /gene="murD"
                     /locus_tag="LGJ20_004994"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK2388955.1"
                     /translation="TSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCV
                     VNADDALTMPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQH
                     NYTNALAALALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGS
                     TEAALNGLHVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDG"
BASE COUNT          149 a          149 c          185 g          142 t
ORIGIN      
        1 ccacctccag cttacaggcg gtagcagcga ccattctgaa cgtgactgaa gatcatatgg
       61 atcgctatcc gtttggttta caacagtatc gtgcagcaaa actgcgcatt tacgaaaacg
      121 cgaaagtttg cgtggttaat gctgatgatg ccttaacaat gccgattcgc ggtgcggatg
      181 aacgctgcgt cagctttggc gtcaacatgg gtgactatca cctgaatcat cagcagggcg
      241 aaacctggct gcgggttaaa ggcgagaaag tgctgaatgt gaaagagatg aaactttccg
      301 ggcagcataa ctacaccaat gcgctggcgg cgctggcgct ggcagatgct gcagggttac
      361 cgcgtgccag cagcctgaaa gcgttaacca cattcactgg tctgccgcat cgctttgaag
      421 ttgtgctgga gcataacggc gtacgttgga ttaacgattc gaaagcgacc aacgtcggca
      481 gtacggaagc ggcgctgaat ggcctgcacg tagacggcac actgcatttg ttgctgggtg
      541 gcgatggtaa atcggcggac tttagcccac tggcgcgtta cctgaatggc gataacgtac
      601 gtctgtattg tttcggtcgt gacgg
//
LOCUS       DAFATA010000331          618 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.339, whole genome shotgun sequence.
ACCESSION   DAFATA010000331 DAFATA010000000
VERSION     DAFATA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.339"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            90..530
                     /locus_tag="LGJ20_004995"
     CDS             90..530
                     /locus_tag="LGJ20_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="HBK2388956.1"
                     /translation="MMVGAGTNYKSMAYVGNIVEFIKYKLKNVTAGYEAYNYVDKPDL
                     NMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAVSSVRVKKFCAT
                     TQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
BASE COUNT          185 a          123 c          145 g          165 t
ORIGIN      
        1 taaccatcat ccgtcctacc gttatcttcg gtgaacgcaa ccgcggtaac gtctataact
       61 tgctgaaaca gatcgctggc ggcaagttta tgatggtggg cgcagggact aactataagt
      121 ccatggctta tgttggaaac attgttgagt ttatcaagta caaactgaag aatgttaccg
      181 caggttacga ggcttataac tacgttgata agccagacct gaatatgaac cagttggttg
      241 ctgaagttga acaaagtctg aacaaaaaga tcccttctat gcacttgcct tacccattag
      301 gaatgctggg tggatattgc tttgatatcc tgagcaaaat tacgggcaaa aaatacgctg
      361 tcagctctgt gcgcgtgaaa aaattctgcg caacaacaca gtttgacgca acgaaagtgc
      421 attcttcagg ttttgtggca ccgtatacgc tgtcgcaagg tctggatcga actctgcagt
      481 atgaattcgt ccatgccaaa aaagacgaca taacgtttgt ttctgagtaa tgcagacgag
      541 tcagagaaaa aaggaccagg ttggactacg attattcgga aagaggtact gattaaatta
      601 tcagtatgtt gatttccg
//
LOCUS       DAFATA010000332          612 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.340, whole genome shotgun sequence.
ACCESSION   DAFATA010000332 DAFATA010000000
VERSION     DAFATA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.340"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>612
                     /gene="pyrC"
                     /locus_tag="LGJ20_004996"
     CDS             <1..>612
                     /gene="pyrC"
                     /locus_tag="LGJ20_004996"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK2388957.1"
                     /translation="LAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNE
                     LERGFNEGVFTAAKLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHAD
                     IDIFDREARFIESVMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLM
                     FNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVASGFNRVFL"
BASE COUNT          148 a          174 c          149 g          141 t
ORIGIN      
        1 atctggctcc gcccgtgacc accgttgagg ctgccgtggc gtatcgccaa cgtattcttg
       61 acgccgtacc tgccgggcac gacttcaccc cattgatgac ctgttattta acagattcgc
      121 tggatcctaa tgagctggag cgcggattta acgaaggcgt gttcaccgct gcaaaacttt
      181 acccggcaaa cgcaaccact aactccagcc acggcgtgac gtcaattgac gcaatcatgc
      241 cggtacttga gcgcatggaa aaaatcggta tgccgctact ggtgcatggt gaagtgacac
      301 atgcagatat cgacattttt gatcgtgaag cgcgctttat agaaagcgtg atggaaccgc
      361 tgcgccagcg cctgactgca ctgaaagtcg tttttgaaca catcaccacc aaagacgcag
      421 ccgactatgt ccgtgacgga aatgaacgac tggctgctac catcactccg caacatctga
      481 tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc gcacctgtat tgtctaccca
      541 tcctcaaacg caatattcac caacaggcac tgcgtgaact ggtcgccagc ggttttaatc
      601 gagtattcct cg
//
LOCUS       DAFATA010000333          609 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.341, whole genome shotgun sequence.
ACCESSION   DAFATA010000333 DAFATA010000000
VERSION     DAFATA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.341"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>609
                     /locus_tag="LGJ20_004997"
     CDS             <1..>609
                     /locus_tag="LGJ20_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388958.1"
                     /translation="ALPGVLSMATAGGMDLGETADIGSNILTQFGLSADQMDRVGDTL
                     TAAFTRTNTDLRALGETMKYAGPVAGKLGISLEQAAAMAGVLANMGIRGSDAGTAMRA
                     SLARLASPPKAAAEALKELGVSVSDAGGKMRPMEDVLADLYKATRKYGEVDRVSFFKD
                     IAGEEAFTSFMALVDAAGDGSLPKLRKELEGARGEAERTAKVM"
BASE COUNT          132 a          151 c          204 g          122 t
ORIGIN      
        1 gcgctccctg gcgtactgag catggcaacg gctggcggta tggacctcgg tgagacggcg
       61 gatattggct caaatattct gacgcagttc ggtctttctg ctgaccagat ggaccgggtc
      121 ggcgacacac tcaccgcagc gtttacccgt accaacactg accttcgcgc actgggtgaa
      181 accatgaaat atgcaggtcc ggtggcgggt aagctgggaa tatcgctgga gcaggccgca
      241 gcgatggcgg gcgtgctggc gaatatgggt atcaggggga gtgatgctgg tacggcaatg
      301 cgtgccagcc tggctcgtct ggcatcaccg ccaaaggcgg cagcagaggc gctgaaagag
      361 cttggcgtgt ctgtctcgga tgccgggggc aaaatgcgcc cgatggagga tgtgctggct
      421 gacctttata aagctacccg caaatacggg gaagttgacc gggtatcgtt ctttaaggac
      481 attgccggag aagaggcttt cacatcattt atggccctcg ttgatgcggc aggtgacggc
      541 tccttaccca aactgagaaa agaacttgaa ggcgcgcgcg gtgaggctga acgcacggca
      601 aaggttatg
//
LOCUS       DAFATA010000334          604 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.342, whole genome shotgun sequence.
ACCESSION   DAFATA010000334 DAFATA010000000
VERSION     DAFATA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.342"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            45..604
                     /locus_tag="LGJ20_004998"
                     /pseudo
     CDS             45..604
                     /locus_tag="LGJ20_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          165 a          165 c          157 g          117 t
ORIGIN      
        1 gacacgcggc taagtgagta aactctcagt cagaggtgac tcacatgaca aaaacagtat
       61 caaccagtaa aaaaccccgt aaacagcatt cgcctgaatt tcgcagtgaa gccctgaagc
      121 ttgctgaacg catcggtgtt actgccgcag cccgtgaact cagcctgtat gaatcacagc
      181 tctacaactg gcgcagtaaa cagcaaaatc agcagacgtc ttctgaacgt gaactggaga
      241 tgtctaccga gattgcacgt ctcaaacgcc agctggcaga acgggatgaa gagctggcta
      301 tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa atgaagtatg tctttattga
      361 aaaacatcag gctgagttca gcatcaaagc aatgtgccgc gtgctccggg tggcccgcag
      421 cggctggtat acgtggtgtc agcggcggac aaggataagc acgcgtcagc agttccgcca
      481 acactgcgac agcgttgtcc tcgcggcttt tacccggtca aaacagcgtt acggtgcccc
      541 acgcctgacg gatgaactgc gtgctcaggg ttaccccttt aacgtaaaaa ccgtggcggc
      601 aagc
//
LOCUS       DAFATA010000335          597 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.343, whole genome shotgun sequence.
ACCESSION   DAFATA010000335 DAFATA010000000
VERSION     DAFATA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.343"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            1..597
                     /locus_tag="LGJ20_004999"
                     /pseudo
     CDS             1..597
                     /locus_tag="LGJ20_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          141 a          159 c          179 g          118 t
ORIGIN      
        1 gatgtcagac tccctgaaca gttctcgata atcgggaaac tcagggcgcg ttatcctgtg
       61 gccactctct gccatgtgtt cggggtccat cgcagcagct acaaatactg gaaaaaccgt
      121 cctgaaaagc cagacggcag acgggctgta ttacgcagcc aggtacttga actgcatggc
      181 atcagccacg gctctgccgg agcaagaagc atcgccacaa tggcaaccca gagaggctac
      241 cagatggggc gctgacttgc tggcagactc atgaaagagc tggggctggt cagttgccag
      301 cagccgactc accggtataa gcgtggcggt catgagcacg ttgctatccc gaatcatctt
      361 gagcgacagt tcgccgtaac ggaaccaaat caggtgtggt gcggtgatgt gacctatatc
      421 tggacgggta agcgctgggc gtacctcgcc gttgttctcg acctgttcgc aagaaaacca
      481 gtgggctggg ccatgtcgtt ctcgccggac agcaggctta ccatgaaagc actggaaatg
      541 gcatgggaaa cccgtggtaa gcccgtcggg gtgatgttcc acagcgatca aggcagt
//
LOCUS       DAFATA010000336          596 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.344, whole genome shotgun sequence.
ACCESSION   DAFATA010000336 DAFATA010000000
VERSION     DAFATA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.344"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>596)
                     /locus_tag="LGJ20_005000"
     CDS             complement(<1..>596)
                     /locus_tag="LGJ20_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBK2388959.1"
                     /translation="QLIRQNNDIERSGANNVGLTALQGLSGIASVFQQEKQAQRQKEF
                     QQAYANAYASGDRSALRNLATQYPDQIESVRKGMGFIDEDQRNSIGTLAAGARLASSS
                     PEAMQSWLQNNANEMARVGVDPNNVAKMYQQNPSGFGEFVDHLGMAALGPIDYFNVQD
                     KMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQN"
BASE COUNT          148 a          160 c          128 g          160 t
ORIGIN      
        1 tgttctgtcc acgtatctgg atgtcctgac ctcgagctgt tagtgcttct cctgcctgat
       61 tgctgcggat tgtctctgcc agcctgcctc ggtcaatctc acgaccagcc atcttgtcct
      121 gaacattgaa gtaatcaatc ggaccaagag cagccattcc aaggtgatca acaaactcac
      181 caaatcctga aggattctgc tgatacatct tagcaacgtt attagggtca acaccgacgc
      241 gcgccatctc attggcgttg ttttgcagcc atgattgcat tgcttctgga gaagatgacg
      301 caagacgtgc gccagctgct aatgtaccga tagaattgcg ctggtcttca tcaatgaatc
      361 ccatgccttt acgaacggat tcaatctggt ctggatattg agtagccaaa ttacgcaaag
      421 cactgcgatc accagacgca taagcattag cgtatgcctg ctgaaattct ttctgccgct
      481 gagcctgctt ttcctgctga aaaacactcg ctatacctga aagaccttgc aaagcagtca
      541 gcccaacatt gttagcgcct gaacgctcaa tatcattgtt ctgcctgata agctga
//
LOCUS       DAFATA010000337          587 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.345, whole genome shotgun sequence.
ACCESSION   DAFATA010000337 DAFATA010000000
VERSION     DAFATA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.345"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..526
                     /gene="aslA"
                     /locus_tag="LGJ20_005001"
     CDS             <1..526
                     /gene="aslA"
                     /locus_tag="LGJ20_005001"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HBK2388960.1"
                     /translation="TWEGGVRVPTFVYWKGMIQPRKSDGIVDLADLFPTALDLAGHPG
                     AKVANLVPKTTFIDGVDQTSFFLGTNGQSNRKAEHYFLNGKLAAVRMDEFKYHVLIQQ
                     PYAYTQSGYQGGFTGTVMQTAGSSVFNLYTDPQESDSIGVRHIPMGVPLQTEMHAYME
                     ILKKYPPRAQIKSD"
BASE COUNT          142 a          158 c          153 g          134 t
ORIGIN      
        1 gacctgggaa ggcggcgttc gcgtaccgac tttcgtttac tggaaaggga tgatccaacc
       61 gcgtaaatct gacggtattg tcgatctggc agatctcttc cctaccgcgc tggatctggc
      121 agggcatcct ggagcgaaag tggcgaattt agtgccgaaa accaccttta tcgatggtgt
      181 ggaccagaca tccttcttcc tgggaacaaa tggtcagtct aaccgtaagg ccgagcacta
      241 cttcctcaac ggtaaactcg ctgctgtgcg tatggatgag ttcaagtatc acgtcctgat
      301 tcagcaacct tacgcttata cccagagcgg atatcagggt ggattcaccg gcacagtaat
      361 gcaaacggcg ggatcgtcgg tgtttaacct ctacaccgat ccgcaggaaa gcgactccat
      421 cggcgtgcgc catattccga tgggtgtacc gctacagacc gaaatgcacg cgtatatgga
      481 gatcctgaaa aaatatccac cacgcgcgca gattaaatct gactaagccg gcgctatcgc
      541 tgagatctgc ctttgccgga tgcgatgctg acgcatctta tccagcc
//
LOCUS       DAFATA010000338          576 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.346, whole genome shotgun sequence.
ACCESSION   DAFATA010000338 DAFATA010000000
VERSION     DAFATA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.346"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>576
                     /locus_tag="LGJ20_005002"
     rRNA            <1..>576
                     /locus_tag="LGJ20_005002"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          125 c          177 g          112 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatg
//
LOCUS       DAFATA010000339          568 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.348, whole genome shotgun sequence.
ACCESSION   DAFATA010000339 DAFATA010000000
VERSION     DAFATA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.348"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>568)
                     /locus_tag="LGJ20_005003"
     CDS             complement(<1..>568)
                     /locus_tag="LGJ20_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBK2388961.1"
                     /translation="AAIAQHGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPAL
                     LACSLPQEHIQRVVAFITGNDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNF
                     GIRLSGTGDRWFQAPANAVEGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAI
                     VKFVGGTPADATNNSRRMQAITLGGNPAF"
BASE COUNT          137 a          157 c          159 g          115 t
ORIGIN      
        1 taaatgcggg gttaccaccg agggtgattg cttgcatcct acggctgtta ttggtggcat
       61 cggcaggggt gcctcccaca aatttgacga tcgctggaga tgacgccatc gcgaagccac
      121 ccacacctgc ggtttcagta attgcgctat cacccaggtc agcagcggca tcgtcgacgc
      181 caaatccggg gaagaacaga ccttcaacgg cattggcagg agcctggaac cagcgatcgc
      241 cagtaccgga aagccgaata ccaaaattca ccccgttacg cgccatcacc gtaaccattg
      301 aactgaacgg tacattggcg gcggcatcca tcattgcttt acaggcggcc atcgaaagat
      361 tgaggaagaa gtgatcgtta ccggtgataa atgccacgac gcgctggata tgttcctgtg
      421 gaagagagca ggccagcaat gccgggagca ggcgcttaat cagcaaaccg gtagcggcag
      481 cattgcggtt atgaacttca tcccccatat gcagcgcctg cgccattaat ggcttgagtt
      541 ccagctcgcc gtgttgtgcg attgctgc
//
LOCUS       DAFATA010000340          563 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.349, whole genome shotgun sequence.
ACCESSION   DAFATA010000340 DAFATA010000000
VERSION     DAFATA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.349"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(10..>563)
                     /locus_tag="LGJ20_005004"
     CDS             complement(10..>563)
                     /locus_tag="LGJ20_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HBK2388962.1"
                     /translation="SYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLA
                     VVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQA
                     QLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDY
                     NRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          129 a          169 c          132 g          133 t
ORIGIN      
        1 gacacaggcc taagcgaggt tcttgttttc aaattgttcc ggactgaggc cgccacacca
       61 actgtgccgc cgccaccgat tgtaatcaca ttcgatataa ttaaacaccg ttgcccgcat
      121 tatttcccgg ctgataaagt gttctccatg gatacattcc actttcagcg aatgaaagaa
      181 gctttccacg caggcattat cgtagcagca accttttgcg ctcatacttc cacgcagatt
      241 atgccgcttc agttgcgcct gataatctgc tgaacagtac tggcctccac ggtccgtgtg
      301 aacgataacg ttccggggcc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
      361 cagttgcgcc gtcatgcgtg gcgacattga ccagccaata acggcacgtg accacaggtc
      421 aatgaccact gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcctgc
      481 ccacttctgg ttcgggccac tggcgtaaaa atcctgctcc aacagatttt ctgacacagg
      541 caggccgtgt gcgcggtagc tga
//
LOCUS       DAFATA010000341          561 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.350, whole genome shotgun sequence.
ACCESSION   DAFATA010000341 DAFATA010000000
VERSION     DAFATA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.350"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>561)
                     /locus_tag="LGJ20_005005"
     CDS             complement(<1..>561)
                     /locus_tag="LGJ20_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK2388963.1"
                     /translation="VIEIKAAAICGADMKHYNVDSGSDEFNSIRGHEFAGCIAQVGEK
                     VKDWKVGQRVVSDNSGHVCGVCPACEQGDFLCCTEKVNLGLDNNTWGGGFSKYCLVPG
                     EILKIHRHALWEIPDGVDYEDAAVLDPICNAYKSIAQQSKFLPGQDVVVIGTGPLGLF
                     SVQMARIMGAVNIVVVGLQEDVAVRFP"
BASE COUNT          152 a          158 c          121 g          130 t
ORIGIN      
        1 gggaagcgga ccgccacatc ttcttgcaga ccaacgacga cgatatttac cgcccccata
       61 attcgcgcca tttgtacgga gaacagcccg agtgggccag tgccgatgac gaccacatcc
      121 tgaccaggaa ggaatttcga ttgctgcgcg atggatttgt aggcattaca gatagggtca
      181 agtacggctg cgtcctcata atcaacacca tcagggattt cccacaacgc atgacgatga
      241 attttgagaa tttcaccagg aacaagacaa tatttggaaa aaccaccgcc ccaggtattg
      301 ttatccagac caaggtttac tttttctgta caacacagaa aatcaccttg ttcacaggcc
      361 gggcaaacac cgcaaacgtg accgctgtta tccgacacga cgcgttgccc cactttccag
      421 tctttgactt tttcaccaac ctgcgcaata caacctgcga actcatggcc gcggatagag
      481 ttaaactcat cagaaccgct atcgacattg tagtgcttca tgtctgcgcc gcagattgcc
      541 gcggctttaa tttcaatcac t
//
LOCUS       DAFATA010000342          556 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.351, whole genome shotgun sequence.
ACCESSION   DAFATA010000342 DAFATA010000000
VERSION     DAFATA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.351"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            152..325
                     /locus_tag="LGJ20_005006"
     CDS             152..325
                     /locus_tag="LGJ20_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HBK2388964.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTNTAHNVKARERIPQLNDK
                     QSPALLKKHRGGI"
     gene            322..>556
                     /locus_tag="LGJ20_005007"
     CDS             322..>556
                     /locus_tag="LGJ20_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prop effector"
                     /protein_id="HBK2388965.1"
                     /translation="MTKRQRKNRKRINRLVELWPELFNREKPQPLKVEIPDDLIQDIA
                     IRELAFGAGALRAAVASYVRSPRYYRALMAGGAR"
BASE COUNT          162 a          147 c          134 g          113 t
ORIGIN      
        1 gtctgataac gaatacgctc actacgcaca gcactgaaca ataccggcaa cagcatgtct
       61 tacatgacat tcgctgtgcc atccaccgga aaacattttc agataccgga tgaggtacca
      121 cttccattga cagcacgagg taacaatact gatggtaacg ctgaccatta accgtaaacc
      181 gcagggtatt ttcagtaagt caccggcaac gccgctgcag gataaaacga acactgcaca
      241 caacgtgaag gccagggaac ggataccaca gttgaacgat aaacagagcc ctgctctgct
      301 gaaaaaacac cgtggcggca tatgacaaaa cgccagcgta aaaaccgtaa gcgtattaat
      361 cgtcttgtgg aactctggcc tgagttattc aaccgggaaa aaccccaacc gcttaaagtt
      421 gagatacctg acgacctgat acaggatatc gccatcagag aactggcgtt tggcgcaggt
      481 gcattacgcg cggcagtagc gtcttatgtg cggtctccgc gctattaccg tgcgttaatg
      541 gctggcggcg cccgtt
//
LOCUS       DAFATA010000343          534 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.355, whole genome shotgun sequence.
ACCESSION   DAFATA010000343 DAFATA010000000
VERSION     DAFATA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.355"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            87..335
                     /locus_tag="LGJ20_005008"
     CDS             87..335
                     /locus_tag="LGJ20_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388966.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(332..>534)
                     /locus_tag="LGJ20_005009"
     CDS             complement(332..>534)
                     /locus_tag="LGJ20_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388967.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          136 a          119 c          126 g          153 t
ORIGIN      
        1 agacttctgg ttcgctcaat ggttcttaca caaacttagg ttcacataga ggttcattct
       61 cagggcggaa ttcagggggc agtacattgt ttatttatgc atctggaggt aatggaggat
      121 ctgccggagg tgcatgtgca aatacatccc gactgcaggg atatgttggt gggacgctaa
      181 ttagcgtgaa tgccagcaat aaccctgcat atggaaaaac agcctttatc agctttgctg
      241 tacctgcagg tacttcctat cagataacat cctatccaac agaaaataca tcatgtggtg
      301 ccggggtatt ttcagtattt ggatatcaaa cttaattgag cgttacacac gacgcaatac
      361 aacctgtctt atgctcgtat ttgtaccatt tgcgtgaacc tgctgggctc tcaactacct
      421 ggcagtagtg ggcatcctct gcgttgccca ttctggcaat ggcacagaag tcgaattgcc
      481 ccagataacc tgtggtggaa agctgagtaa ctttgagttt tccacctatt gtcc
//
LOCUS       DAFATA010000344          531 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.357, whole genome shotgun sequence.
ACCESSION   DAFATA010000344 DAFATA010000000
VERSION     DAFATA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.357"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>531)
                     /locus_tag="LGJ20_005010"
     CDS             complement(<1..>531)
                     /locus_tag="LGJ20_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBK2388968.1"
                     /translation="ITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTL
                     PGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRS
                     FTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSL
                     DRREVLHTQGEAGLKRV"
BASE COUNT           82 a          172 c          162 g          115 t
ORIGIN      
        1 acccgcttca gcccggcttc gccctgcgtg tgcagcactt cacggcggtc caggctgtcg
       61 gtgatggtga tgcggtcttt ctcatactga tacgtgtagc ttaagcctgc cgggtttagc
      121 tgttctgtca cccgcccgtc gctgtcgtaa cggtaacgga tttccggtcg gcccgtgtga
      181 cggtgcgcca ccatccggcc ccggtattta tcatcgtaag taaagctgcg cacctgtgta
      241 ttgctgcggt catacactgc cgccagttcg ccgcgcggcg tccagccata gcgcaccagc
      301 ggcgcggcag gtaaattctc cgggtattcc gggtcgtgcg tcagccacac ggcagacaga
      361 cggatgccgt tgtcccggcc atattcggtg taacccggca gggtatcagg aaaagcggac
      421 ggttccgtcc cgccggaaat ggcctgctgc cgggcttctt ctgcccgctg cgcctgcgtg
      481 gtcagtacca gccggaagtg acgcccggca ccatccgtca cgccggtgat t
//
LOCUS       DAFATA010000345          523 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.358, whole genome shotgun sequence.
ACCESSION   DAFATA010000345 DAFATA010000000
VERSION     DAFATA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.358"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..239)
                     /locus_tag="LGJ20_005011"
     CDS             complement(<1..239)
                     /locus_tag="LGJ20_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBK2388969.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAW"
BASE COUNT          127 a          126 c          130 g          140 t
ORIGIN      
        1 taccacgcat caggttcatc atgaatttcg atacctttga tccggttgat gatcaccgtg
       61 ccgcgatagt cttccagaaa aagtacatta cttccttcac ccagaataag aacgggttgt
      121 ccttctgcgg ttgcatactg ccaggcattg agtaattgtt gttcgtcttc ggcacataca
      181 atgtgctgag cattatgatc aatgccaaat gtgttccagg gttttaagga gtggttcata
      241 gctgctttcc tgatgcaaaa acgaggctag tttaccgtat ctgtgggggg atggcttgta
      301 gatatgacga caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      361 tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
      421 tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
      481 gacaaacaac agataaaacg aaaggcccag tctttcgact gag
//
LOCUS       DAFATA010000346          504 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.360, whole genome shotgun sequence.
ACCESSION   DAFATA010000346 DAFATA010000000
VERSION     DAFATA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.360"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(17..>504)
                     /locus_tag="LGJ20_005012"
     CDS             complement(17..>504)
                     /locus_tag="LGJ20_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK2388970.1"
                     /translation="EKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMV
                     ENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVE
                     CFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPR
                     V"
BASE COUNT          147 a          111 c           88 g          158 t
ORIGIN      
        1 ccaaaaagtt ggacagttaa acacgaggca tataggtctg attccgatat tcaattggag
       61 tcagaccttt taatttcagg ctaattcttc tgctgttgta gtattcaata tattccgtaa
      121 cagcatcctt cagttcgctt atattactga actcatcaag ataaaaacac tccgacttta
      181 aggttccaaa gaaacactcc accacagcat tatccagaca attgcctttt ctggacatgc
      241 tttgtttaat accatgttct ttaaggatat tttgatatct tctcatacga tactgccatc
      301 cctggtcaga gtgcagaaca ggatgctcgt gaggattaag ctttttgaat gcctgatcga
      361 gcatattctc aaccatgttc atcactggtc tttccgaaag gctgtaagaa ataacttcgt
      421 tgttgaagag atctattact ggagacaaat acagcttgcg cccattgact gcaaattcag
      481 taacatcggt aacccacttc tcgt
//
LOCUS       DAFATA010000347          503 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.361, whole genome shotgun sequence.
ACCESSION   DAFATA010000347 DAFATA010000000
VERSION     DAFATA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.361"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>503
                     /gene="phnF"
                     /locus_tag="LGJ20_005013"
     CDS             <1..>503
                     /gene="phnF"
                     /locus_tag="LGJ20_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBK2388971.1"
                     /translation="EIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQL
                     VEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHV
                     ADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQRFDSGSLHDFLREQT
                     GIALRRS"
BASE COUNT           96 a          171 c          145 g           91 t
ORIGIN      
        1 gagatagccg caaaacttga gcaggagctt cgtcaacact accgctgcgg cgactatctt
       61 cccgccgagc agcaactggc agcgcgcttt gaggtgaatc gccacaccct gcgccgcgcc
      121 atcgaccaac tggtggaaaa aggctgggta cagcgccgtc agggcgtcgg cgtgctggtg
      181 ctgatgcgcc cgttcgatta cccgctcaac gcccaggcgc gttttagcca gaatctgctg
      241 gatcagggca gccatcccac cagcgaaaaa ctgctttcgg tattgcgccc cgcgtccggc
      301 cacgtcgctg acgcactggg gattaccgag ggggagaacg tcatccacct gcgcaccctg
      361 cgtcgtgtca acggcgtcgc gctctgttta atcgaccact acttcgcgga cctcaccctc
      421 tggccgacgc tgcaacgctt cgacagcggc tcgctgcacg attttctgcg cgagcaaacc
      481 ggaattgcgc tgcgccgcag cca
//
LOCUS       DAFATA010000348          502 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.362, whole genome shotgun sequence.
ACCESSION   DAFATA010000348 DAFATA010000000
VERSION     DAFATA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.362"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..274
                     /locus_tag="LGJ20_005014"
     CDS             <1..274
                     /locus_tag="LGJ20_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2388972.1"
                     /translation="YKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVC
                     GYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQEPTEMIQATA"
     gene            complement(314..>502)
                     /locus_tag="LGJ20_005015"
     CDS             complement(314..>502)
                     /locus_tag="LGJ20_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBK2388973.1"
                     /translation="STIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLI
                     AGGVCYVVLNLIADRNRY"
BASE COUNT          126 a          143 c          133 g          100 t
ORIGIN      
        1 gtacaagcag ctctggcgtg gcggtcaggt gcttgctgtt ccgccagcgt atacaagcca
       61 gcgttgcgcg tgctgtggtc atacagcgaa agaaaatcgt ctgtcacaaa gtaaattcag
      121 atgccaggta tgtggatata cagcgaacgc cgatgtaaac ggcgctcgta acattttagc
      181 ggcggggcac gccgttcttg cctgtggagg gatggtgcag tcaggccgcc cgttgaagca
      241 ggaacccacc gaaatgattc aggcgacagc ctgaacgtag cagggatcca cgtccttcag
      301 ggcgtggagg atgtcaatat cggtttctgt cagcgattaa attcaacacc acgtaacaaa
      361 cgcctccggc aattaatccc cagaacgcag aaccaatccc gaccagcgtc aatccacttg
      421 ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc agcgcctgat
      481 acaaactgcc gccaatggta ct
//
LOCUS       DAFATA010000349          500 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.363, whole genome shotgun sequence.
ACCESSION   DAFATA010000349 DAFATA010000000
VERSION     DAFATA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.363"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            286..>500
                     /locus_tag="LGJ20_005016"
     CDS             286..>500
                     /locus_tag="LGJ20_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HBK2388974.1"
                     /translation="MDEAEGFIFKPSTSKTLNAASHSKNLIDIIDSGCELEDISSIIP
                     ENLSGNIDKLINQTLDFIRGAPDFGGAV"
BASE COUNT          174 a           60 c          101 g          165 t
ORIGIN      
        1 ctacaaggaa tgttgtaaag gatcagcagt tattgaaaca attacgtaaa gtagattcag
       61 gaaaatagag taaagtttat aaagatggat ttgatgcgtc aggtcgaaaa gtgtccattc
      121 actattttga aagtcagagt ggtcgagtat ttaatgtaaa agtaaaacct tattggagta
      181 atttataatg agaattgatt atagtattag caatgaacac gcagagaaat ttatcagtct
      241 tttagtgttg ggtgttttat attctattga aaaaaagcaa tatctataga tgaggcagaa
      301 ggttttatat ttaagccatc tacgtcaaaa acattgaatg ctgcatcgca ctctaaaaac
      361 ttaattgaca ttatagattc tggttgtgag cttgaagata tatctagcat cattcctgaa
      421 aatttatcag gtaatattga taaattaatt aaccagacgc tggattttat tcgaggtgct
      481 cctgattttg gcggtgcagt
//
LOCUS       DAFATA010000350          491 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.365, whole genome shotgun sequence.
ACCESSION   DAFATA010000350 DAFATA010000000
VERSION     DAFATA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.365"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..342)
                     /locus_tag="LGJ20_005017"
     CDS             complement(<1..342)
                     /locus_tag="LGJ20_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388975.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKD"
     gene            complement(372..>491)
                     /locus_tag="LGJ20_005018"
     CDS             complement(372..>491)
                     /locus_tag="LGJ20_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBK2388976.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          119 a          161 c           72 g          139 t
ORIGIN      
        1 atccttaata gacctgtagg tatttcttat ttcgccatcc tcatctggct cccaccctcc
       61 cgaacctcct gtactgccac tgcccaaaca gttcttctct tcatccgtca aatccttccc
      121 gacatttgga ttctcttttg atgaatcatc atttccaaac acgctggaaa aagcaccata
      181 gggatctcgc cagaattcca tttgactttg tgcatttaat aattcggctt caccccgcat
      241 ctccatactt ttctcaatcg catccccaac agcctcgcga ttgttgtata ttactatacc
      301 accaccaata accaccgctc caactgccgc accgactaac atccctggag ggcctcctac
      361 ccagggcaag attacgaaag cccgctcccc gcaaggactg acgccagata gttcctgtcc
      421 atggctgctt ttcgcatctt acgtcttaac cctgccttga ataccttatc attcgtcaaa
      481 atattaatag c
//
LOCUS       DAFATA010000351          485 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.367, whole genome shotgun sequence.
ACCESSION   DAFATA010000351 DAFATA010000000
VERSION     DAFATA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.367"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>485
                     /locus_tag="LGJ20_005019"
     rRNA            <1..>485
                     /locus_tag="LGJ20_005019"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAFATA010000352          483 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.369, whole genome shotgun sequence.
ACCESSION   DAFATA010000352 DAFATA010000000
VERSION     DAFATA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.369"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            86..>483
                     /locus_tag="LGJ20_005020"
     CDS             86..>483
                     /locus_tag="LGJ20_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBK2388977.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPL"
BASE COUNT          120 a          125 c          147 g           91 t
ORIGIN      
        1 gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttt ccggtgaaaa
       61 ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcgt tgcttttgag
      121 tgtgggagcg tcatcggtct tcgcggcagg aaccgtcaag gtgttcagca atggcagcag
      181 tgaggccaaa acgctaacgg gcgcagagca tttaatcgat ctggtaggcc aaccgcggct
      241 ggcaaacagc tggtggcccg gtgcggtgat tagcgaagag ctggcaacgg cggcagcatt
      301 gcgtcagcag caggcgttgc tgacacggct ggcagaacag ggcgcagatt ccagcgccga
      361 cgatgccgct gcgattaacg ccttacgcca gcaaattcag gcgttgaaag tgacgggcag
      421 gcaaaaaatc aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta acccgccgtt
      481 gca
//
LOCUS       DAFATA010000353          477 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.371, whole genome shotgun sequence.
ACCESSION   DAFATA010000353 DAFATA010000000
VERSION     DAFATA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.371"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            1..477
                     /locus_tag="LGJ20_005021"
                     /pseudo
     CDS             1..477
                     /locus_tag="LGJ20_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
BASE COUNT           99 a          152 c          130 g           96 t
ORIGIN      
        1 cccgcacgcg gcagcaactc agcaacatca tgcgctatgc cgtatagcag gggcttaccg
       61 aaagcaatcc ggcgcagcat ctggatggcg ttgtctcctc ccccgtaaag aatcactacc
      121 ccgcgttacc gctggagcgg ttgtccgaac tgcttggccg catcggtgac tatcagcagg
      181 gccgggagtt aacccggctg gcggaggtgc tgacactgca cctgttcatc cgttccagcg
      241 aactgcgttt cgcccgctgg agcgagatcg atttcaggca caaaatctgg accatccccg
      301 ccacccgcga ggccatcgac aaagttcggt tttcggggcg tggcgcaaaa atgcgtactc
      361 cgcatatcgt accgctctcc cgccaggcga ttgcccttct gaaacagata caggagcttt
      421 ccggccatct ggatctggtg tttcccggcg atcataatcc gtacaagcca atgagcg
//
LOCUS       DAFATA010000354          469 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.374, whole genome shotgun sequence.
ACCESSION   DAFATA010000354 DAFATA010000000
VERSION     DAFATA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.374"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>469
                     /locus_tag="LGJ20_005022"
     CDS             <1..>469
                     /locus_tag="LGJ20_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBK2388978.1"
                     /translation="GEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMV
                     ASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHT
                     TLPLFEKLVQREPVTLVSLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQY"
BASE COUNT          103 a          142 c          147 g           77 t
ORIGIN      
        1 acggcgaagg cggctggctg ctgccggggc tgattgagct gcataccgat aatctggata
       61 aattcttcac cccgcgcccg aaagttgact ggcctgccca ctcggcgatg agcagccacg
      121 acgcgctgat ggtggcgagc ggcatcacca ccgtactgga tgccgtggca attggcgacg
      181 tgcgcgacgg cggcgatcgg ctggagaatc tggagaagat gatcaacgcc atcgaagaga
      241 cgcagaaacg cggcgtcaac cgcgccgagc accgtctgca tctgcgctgc gaactgccgc
      301 atcacaccac gctgccgctg tttgaaaaac tggtgcagcg cgagccggtg acgctggtgt
      361 cgctgatgga ccactcgccg ggccagcgcc agttcgccaa ccgcgagaag tatcgcgaat
      421 attatcaggg caaatactcc ctcactgatg cgcagatgca gcagtacga
//
LOCUS       DAFATA010000355          464 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.375, whole genome shotgun sequence.
ACCESSION   DAFATA010000355 DAFATA010000000
VERSION     DAFATA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.375"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>464
                     /gene="ribD"
                     /locus_tag="LGJ20_005023"
     CDS             <1..>464
                     /gene="ribD"
                     /locus_tag="LGJ20_005023"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBK2388979.1"
                     /translation="AQRGRFTTHPNPNVGCVIVKDGEIVGEGYHHRAGEPHAEVHALR
                     MAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQDPNPQVAGRGLYRL
                     QQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGRTAMASGE"
BASE COUNT          105 a          116 c          141 g          102 t
ORIGIN      
        1 gcgcagcggg ggcgttttac cactcatccc aacccgaatg ttgggtgtgt cattgtgaaa
       61 gatggcgaaa tcgtcggtga aggttaccat caccgtgctg gtgaaccaca cgccgaagta
      121 catgcgttgc gtatggctgg agaaaaagcc aaaggtgcga ctgcgtatgt gacactcgaa
      181 ccttgcagcc atcatggccg tacgccgcct tgctgtgatg cattaattgc cgcaggtgtg
      241 gcgcgcgtgg ttgcctcgat gcaagatcct aacccgcagg tagccgggcg agggctatat
      301 cgcctgcaac aagcgggtat tgacgtcagc cacggcctga tgatgagtga agccgaacaa
      361 ttgaataaag gctttctcaa gcggatgcgt accggctttc cttatatcca gttaaaactt
      421 ggcgcatcgc ttgatggtcg tacggcgatg gccagcggtg agag
//
LOCUS       DAFATA010000356          454 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.376, whole genome shotgun sequence.
ACCESSION   DAFATA010000356 DAFATA010000000
VERSION     DAFATA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.376"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>454
                     /locus_tag="LGJ20_005024"
     CDS             <1..>454
                     /locus_tag="LGJ20_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBK2388980.1"
                     /translation="QTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTR
                     TLTLDREITLPSSGTTLISLVDGQGSPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGL
                     KLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQS"
BASE COUNT           94 a          124 c          154 g           82 t
ORIGIN      
        1 cagaccgtgg acttcagcgt gggtgccgaa gggcttcgcc atgtaccggg cgatgtcatt
       61 gaaatctgcg atgatgacta tgccggtatc agcaccggtg ggcgcgtgct ggcggtaaac
      121 agccagaccc ggacgctgac gctcgaccgt gaaatcacgc tgccatcttc cggcaccacg
      181 ctgataagcc tggttgacgg gcaggggagt ccggtcagcg tggaggttca gtccgtcacc
      241 gacggcgtga aggtaaaagt gagccgtgtt cctgacggtg ttgctgaata cagcgtatgg
      301 gggctgaagc tgccgacgct gcgccagcga ctgttccgct gcgtgagtat ccgtgagaac
      361 gacgacggca cgtatgccat caccgccgtg cagcatgtgc cggaaaaaga ggccatcgtg
      421 gataacgggg cgcactttga cggcgaccag agca
//
LOCUS       DAFATA010000357          454 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.377, whole genome shotgun sequence.
ACCESSION   DAFATA010000357 DAFATA010000000
VERSION     DAFATA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.377"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            1..454
                     /locus_tag="LGJ20_005025"
                     /pseudo
     CDS             1..454
                     /locus_tag="LGJ20_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          100 a          115 c          143 g           96 t
ORIGIN      
        1 ggatgccggg caactgcccg cattatgggc gttggcctca acacgatttt acgtcactta
       61 aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct
      121 gcgcggaaat ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt
      181 tttacgcgta tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta
      241 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
      301 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
      361 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
      421 gacggaagtc gctgtcgttc tcaaaatcgg tgga
//
LOCUS       DAFATA010000358          453 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.378, whole genome shotgun sequence.
ACCESSION   DAFATA010000358 DAFATA010000000
VERSION     DAFATA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.378"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>453)
                     /locus_tag="LGJ20_005026"
     rRNA            complement(<1..>453)
                     /locus_tag="LGJ20_005026"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          145 c           98 g          127 t
ORIGIN      
        1 ccttctcccg aagttacggc accattttgc ctagttcctt cacccgagtt ctctcaagcg
       61 ccttggtatt ctctacctga ccacctgtgt cggtttgggg tacgatttga tgttacctga
      121 tgcttagagg cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc
      181 atcacgcctc agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc
      241 gggacaaccg tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag
      301 tacaggaata ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga
      361 ctcaccctgc cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt
      421 cacccgcttt atcgttactt atgtcagcat tcg
//
LOCUS       DAFATA010000359          436 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.383, whole genome shotgun sequence.
ACCESSION   DAFATA010000359 DAFATA010000000
VERSION     DAFATA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.383"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>436)
                     /locus_tag="LGJ20_005027"
     CDS             complement(<1..>436)
                     /locus_tag="LGJ20_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388981.1"
                     /translation="FIVVDDEDKTRHQYIAEFGEQVLVFSKSDIASRFDEADNFCDRR
                     SIFYARNACFDLAKLVGCKYFIQLDDDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLA
                     YYESIPALSIAMSQGGDFLGDNGGHASWVKRKAMNSFICSVDR"
BASE COUNT          132 a          109 c           94 g          101 t
ORIGIN      
        1 cgatcaaccg aacagataaa gctgttcatt gccttgcgtt tcacccacga agcatggccg
       61 ccattgtcac caagaaaatc cccgccctgc gacatagcga tggaaagagc aggtattgat
      121 tcgtagtacg ccagcatttc agaaaggatc gcatccagtt tccttatcgg aaaataggcc
      181 tggtcatagt tgcgatccac ccgaaactgg aactcgtgat aatcatcatc gagctgaatg
      241 aagtatttac acccgaccag ttttgccagg tcgaaacagg cattacgggc gtaaaaaatt
      301 gagcggcggt cacagaaatt atcggcttcg tcaaaacgac tggcgatatc ggatttggaa
      361 aacaccagca cctgttcacc aaattcagct atgtactgat gccgtgtctt atcttcatca
      421 tcaacaacga taaaaa
//
LOCUS       DAFATA010000360          431 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.385, whole genome shotgun sequence.
ACCESSION   DAFATA010000360 DAFATA010000000
VERSION     DAFATA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.385"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..>431)
                     /locus_tag="LGJ20_005028"
     CDS             complement(<1..>431)
                     /locus_tag="LGJ20_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2388982.1"
                     /translation="QWLTRVVSKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLA
                     IGVLAGPLAKLRLGFSLLTGSMNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVA
                     ALAGAAVLIWKYWDPIRAFFAGVFSGIMERLTPLRETFERFG"
BASE COUNT          116 a          141 c          108 g           66 t
ORIGIN      
        1 ccaaaccgtt caaaggtttc gcgcaacggg gtcagccttt ccataatccc gctgaacacc
       61 ccggcaaaaa atgccctgat gggatcccag tatttccaga taagaacggc agctccggca
      121 agcgcagcca cgataagacc aaccggactg aacaacgccc cgatagcgcc tcccagtaaa
      181 gaaacggaac ccgtcaccat tccccatagt gctggcagaa ccctgacagc attcattgat
      241 ccggtcagga gggaaaaacc aagacgcagt tttgccagcg ggccagcaag cacaccaata
      301 gccagcgaca acgagccaac cgttgcagtc attgccagca gtgcaccgcc tgcaatcagt
      361 agctggcgcg tcagtgcggg atgggcctgc gccagcgccg tcacctttga taccacacgc
      421 gtgagccact g
//
LOCUS       DAFATA010000361          418 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.388, whole genome shotgun sequence.
ACCESSION   DAFATA010000361 DAFATA010000000
VERSION     DAFATA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.388"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>418
                     /locus_tag="LGJ20_005029"
     CDS             <1..>418
                     /locus_tag="LGJ20_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HBK2388983.1"
                     /translation="KETLETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQ
                     ETGQPSSEYRLALWEKLGQPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTW
                     ISYQIMVNLGLLFALSAPVTALGLPIAFVLVALSLVY"
BASE COUNT           88 a          106 c          108 g          116 t
ORIGIN      
        1 aaagaaacgc tggaaacatt agataatctt gcctggcaat cggccttcac cagtatggat
       61 cttgaagagt tatcaatgcc gggcaatact ttttctgtcc gtcagcttaa tcattacatc
      121 cattatttgc aggaaaccgg gcaacccagc agcgaatacc gccttgcact gtgggaaaaa
      181 ctggggcaac ccatcctgac cctggcgatg attttgctgg ctgtgccgtt tacctttagc
      241 gccccgcgct cgccagggat gggtagccgt ctcgctgtag gtgtcatcgt tggcttactc
      301 acctggatca gctatcaaat catggtcaat ctgggattgc tatttgcgtt gagcgcacct
      361 gttaccgcgc tcggtttacc gatagcgttt gtgctggtgg cgttgagcct ggtgtatt
//
LOCUS       DAFATA010000362          413 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.390, whole genome shotgun sequence.
ACCESSION   DAFATA010000362 DAFATA010000000
VERSION     DAFATA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.390"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            1..413
                     /locus_tag="LGJ20_005030"
                     /pseudo
     CDS             1..413
                     /locus_tag="LGJ20_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           92 a          107 c          124 g           90 t
ORIGIN      
        1 ggatgccggg caaccgcccg cattatgggc gttggcctca acacgatttt ccgccattta
       61 aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct
      121 gcgcggaaat ggacgaacag tggggctacg tcggtgctaa atcacgtcag cgctggctgt
      181 tttacgcgta tgacaggata cggaggacag ttgtggcgca cgttttcggt gaacgcactc
      241 tggccacact ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatggatga
      301 cggatggctg gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt atcagcaagc
      361 gttacactca gcgcattgag cgacataatc tgaatctgag acaacatctg gca
//
LOCUS       DAFATA010000363          395 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.399, whole genome shotgun sequence.
ACCESSION   DAFATA010000363 DAFATA010000000
VERSION     DAFATA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.399"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>395
                     /locus_tag="LGJ20_005031"
     CDS             <1..>395
                     /locus_tag="LGJ20_005031"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="catalyzes the formation of
                     3-(imidazol-4-yl)-2-oxopropyl phosphate from
                     D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and
                     histidinol from histidinol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HBK2388984.1"
                     /translation="YIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLAR
                     CALDISGRPHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRV
                     ESLFKAFGRTLRQAIRVEGDTLPSSKGVL"
BASE COUNT           88 a          119 c          105 g           83 t
ORIGIN      
        1 ctatatcgac gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgctaaa
       61 aatcgccctc ggagacaaac gcggtatttg ccgctttggt tttgtgctac cgatggacga
      121 atgccttgcc cgctgcgcgc tggatatctc tggtcgcccg cacctggaat ataaagccga
      181 gtttacctac cagcgcgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc
      241 gctctcttac actatgggtg tgacgctaca cctgaaaacc aaaggtaaaa acgatcacca
      301 ccgtgtagag agcctgttca aagcctttgg tcggaccctg cgccaggcca tccgcgtgga
      361 aggcgacacc ctgccctcgt cgaaaggagt gctgt
//
LOCUS       DAFATA010000364          393 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.401, whole genome shotgun sequence.
ACCESSION   DAFATA010000364 DAFATA010000000
VERSION     DAFATA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.401"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..>393
                     /locus_tag="LGJ20_005032"
     CDS             <1..>393
                     /locus_tag="LGJ20_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2388985.1"
                     /translation="RSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDL
                     TATISPDGKRSTIAYDKRGRPVSVTEGGL"
BASE COUNT          101 a          102 c          130 g           60 t
ORIGIN      
        1 gcgcagcggt gagacaacgc ggtacagcta tgatgacccg gcgagtgagc tgccgacagg
       61 gatacaggac gcgacgggca gtacaaaaca gatggcatgg agccgttacg gtcaactgct
      121 ggccttcacg gactgctcgg ggtacacgac gcggtatgag tacgaccggt acggtcaaca
      181 aatcgccgtt caccgggagg aaggcatcag cacttacagc agttataacc cgcgtgggca
      241 actggtcagt cagaaggatg cgcaggggcg tgaaatccgt tatgagtaca gcgccgcagg
      301 cgacctgaca gcaacgatat caccggacgg taaacgcagc accatcgcat atgataaacg
      361 cgggcgtccg gtgagtgtga cggaaggcgg tct
//
LOCUS       DAFATA010000365          378 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.denovo.410, whole genome shotgun sequence.
ACCESSION   DAFATA010000365 DAFATA010000000
VERSION     DAFATA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.denovo.410"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..163)
                     /locus_tag="LGJ20_005033"
     CDS             complement(<1..163)
                     /locus_tag="LGJ20_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="involved in rpiB and als operon repression; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2388986.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVA"
BASE COUNT          104 a           74 c           74 g          126 t
ORIGIN      
        1 ctgcgacatc tttaattgcg ggtgcacaac tcaggttacc gggtttgagt aaccactcca
       61 cgatgcggct ttcattttct gtcattcctt cctgcttcat tcgcaggtaa ggcgctaacc
      121 ctataccgtt cggaagcgct gaatcaaact ctgactggct catcacgttc tcttgtcctt
      181 gaattgaaga cgccactata tcaccacagc aagaacagaa aagatgctgg cgacttcaca
      241 aaaaaataaa atttataaag tttattttat tgtgaacaat ggcgagtggc ttaaaatttt
      301 atttcattta attagtttaa aaacatgatg ttatttgttt ttctggtttt ttagatgata
      361 gatgttgctg gatggtga
//
LOCUS       DAFATA010000366        35367 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.guided.041, whole genome shotgun sequence.
ACCESSION   DAFATA010000366 DAFATA010000000
VERSION     DAFATA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.guided.041"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..2450)
                     /locus_tag="LGJ20_005034"
     CDS             complement(<1..2450)
                     /locus_tag="LGJ20_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HBK2388987.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPE
                     GLDRKFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAI
                     NPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALI
                     DQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLT
                     KMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQ
                     RFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP
                     LIGGTLNHGSLAAANRGVA"
     gene            complement(2454..3014)
                     /locus_tag="LGJ20_005035"
     CDS             complement(2454..3014)
                     /locus_tag="LGJ20_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2388988.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            complement(3190..3540)
                     /locus_tag="LGJ20_005036"
     CDS             complement(3190..3540)
                     /locus_tag="LGJ20_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBK2388989.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(3743..4756)
                     /gene="intI1"
                     /locus_tag="LGJ20_005037"
     CDS             complement(3743..4756)
                     /gene="intI1"
                     /locus_tag="LGJ20_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBK2388990.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            4914..5387
                     /locus_tag="LGJ20_005038"
     CDS             4914..5387
                     /locus_tag="LGJ20_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HBK2388991.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            5518..6306
                     /locus_tag="LGJ20_005039"
     CDS             5518..6306
                     /locus_tag="LGJ20_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBK2388992.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            6508..7212
                     /locus_tag="LGJ20_005040"
     CDS             6508..7212
                     /locus_tag="LGJ20_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBK2388993.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            7265..8020
                     /locus_tag="LGJ20_005041"
                     /pseudo
     CDS             7265..8020
                     /locus_tag="LGJ20_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
     gene            complement(8202..8378)
                     /locus_tag="LGJ20_005042"
     CDS             complement(8202..8378)
                     /locus_tag="LGJ20_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBK2388994.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            8708..9523
                     /gene="sul2"
                     /locus_tag="LGJ20_005043"
     CDS             8708..9523
                     /gene="sul2"
                     /locus_tag="LGJ20_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBK2388995.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            9610..9912
                     /locus_tag="LGJ20_005044"
     CDS             9610..9912
                     /locus_tag="LGJ20_005044"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388996.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(10088..11233)
                     /locus_tag="LGJ20_005045"
                     /pseudo
     CDS             complement(10088..11233)
                     /locus_tag="LGJ20_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
     gene            11591..11860
                     /locus_tag="LGJ20_005046"
     CDS             11591..11860
                     /locus_tag="LGJ20_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2388997.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            11857..12138
                     /locus_tag="LGJ20_005047"
     CDS             11857..12138
                     /locus_tag="LGJ20_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2388998.1"
                     /translation="MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            12178..13026
                     /locus_tag="LGJ20_005048"
     CDS             12178..13026
                     /locus_tag="LGJ20_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBK2388999.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NSYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            13143..13616
                     /locus_tag="LGJ20_005049"
     CDS             13143..13616
                     /locus_tag="LGJ20_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389000.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(14093..14359)
                     /locus_tag="LGJ20_005050"
     CDS             complement(14093..14359)
                     /locus_tag="LGJ20_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389001.1"
                     /translation="MLTGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(14359..14853)
                     /locus_tag="LGJ20_005051"
     CDS             complement(14359..14853)
                     /locus_tag="LGJ20_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389002.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(14909..15511)
                     /locus_tag="LGJ20_005052"
     CDS             complement(14909..15511)
                     /locus_tag="LGJ20_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBK2389003.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            15865..17211
                     /locus_tag="LGJ20_005053"
     CDS             15865..17211
                     /locus_tag="LGJ20_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389004.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            17490..19370
                     /locus_tag="LGJ20_005054"
     CDS             17490..19370
                     /locus_tag="LGJ20_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B"
                     /protein_id="HBK2389005.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AINSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     NIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWAFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(19388..19735)
                     /locus_tag="LGJ20_005055"
     CDS             complement(19388..19735)
                     /locus_tag="LGJ20_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HBK2389006.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
     gene            19854..20202
                     /locus_tag="LGJ20_005056"
                     /pseudo
     CDS             19854..20202
                     /locus_tag="LGJ20_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20222..20812)
                     /locus_tag="LGJ20_005057"
     CDS             complement(20222..20812)
                     /locus_tag="LGJ20_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389007.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(20812..21069)
                     /locus_tag="LGJ20_005058"
     CDS             complement(20812..21069)
                     /locus_tag="LGJ20_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389008.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            21420..22421
                     /locus_tag="LGJ20_005059"
     CDS             21420..22421
                     /locus_tag="LGJ20_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HBK2389009.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            complement(22597..22941)
                     /locus_tag="LGJ20_005060"
     CDS             complement(22597..22941)
                     /locus_tag="LGJ20_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HBK2389010.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKVFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(23440..23694)
                     /locus_tag="LGJ20_005061"
     CDS             complement(23440..23694)
                     /locus_tag="LGJ20_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389011.1"
                     /translation="MKGRQSRYVTGGESFAEIARLPSGAVVRLCLNTGLEDALREASK
                     SLKSAFTRSGRKCRLSAGTAQGPFTGRRQGVATHLFVSVL"
     gene            complement(23739..24665)
                     /locus_tag="LGJ20_005062"
     CDS             complement(23739..24665)
                     /locus_tag="LGJ20_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389012.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADTDRVITDLASHARKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDEELASYLDAKVMEQRYQSHFITRWNQEYQK
                     LLGISTEEELRRLVLNNTPFLQVRADQVLKGWKNIPRGISLTFSLFAEIAGRDRETID
                     QAWKNIFYSQLREKKHRFYQDIEAIRALKKLPALTGDSWTRDGITVRIYRPENYARGA
                     WRVTLSLPENYATQMWNIPFPELEYRLFTADPGYSALISAEPDRWDKAFRFVDGVCEL
                     HLYTNGVEEDHNPTPLGDVAQALINVVEENLL"
     gene            25197..25610
                     /locus_tag="LGJ20_005063"
     CDS             25197..25610
                     /locus_tag="LGJ20_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389013.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            25612..26391
                     /locus_tag="LGJ20_005064"
     CDS             25612..26391
                     /locus_tag="LGJ20_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBK2389014.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            26571..27215
                     /locus_tag="LGJ20_005065"
     CDS             26571..27215
                     /locus_tag="LGJ20_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK2389015.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            27302..27610
                     /locus_tag="LGJ20_005066"
     CDS             27302..27610
                     /locus_tag="LGJ20_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HBK2389016.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            28024..29004
                     /locus_tag="LGJ20_005067"
     CDS             28024..29004
                     /locus_tag="LGJ20_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBK2389017.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            28997..29413
                     /locus_tag="LGJ20_005068"
     CDS             28997..29413
                     /locus_tag="LGJ20_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBK2389018.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(29415..30689)
                     /locus_tag="LGJ20_005069"
     CDS             complement(29415..30689)
                     /locus_tag="LGJ20_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK2389019.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(30689..31126)
                     /gene="umuD"
                     /locus_tag="LGJ20_005070"
     CDS             complement(30689..31126)
                     /gene="umuD"
                     /locus_tag="LGJ20_005070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBK2389020.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(31123..31371)
                     /locus_tag="LGJ20_005071"
     CDS             complement(31123..31371)
                     /locus_tag="LGJ20_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBK2389021.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            31789..32691
                     /locus_tag="LGJ20_005072"
     CDS             31789..32691
                     /locus_tag="LGJ20_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBK2389022.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            32688..32999
                     /locus_tag="LGJ20_005073"
     CDS             32688..32999
                     /locus_tag="LGJ20_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389023.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            33076..33759
                     /locus_tag="LGJ20_005074"
     CDS             33076..33759
                     /locus_tag="LGJ20_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBK2389024.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            33760..33981
                     /locus_tag="LGJ20_005075"
     CDS             33760..33981
                     /locus_tag="LGJ20_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389025.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            33993..34427
                     /locus_tag="LGJ20_005076"
     CDS             33993..34427
                     /locus_tag="LGJ20_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBK2389026.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            34478..35248
                     /locus_tag="LGJ20_005077"
     CDS             34478..35248
                     /locus_tag="LGJ20_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2389027.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSCGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
BASE COUNT         8105 a         9078 c         9570 g         8614 t
ORIGIN      
        1 gccacaccac gatttgcagc cgccaggctg ccgtggttca gggtgccgcc gatcagcggg
       61 cgcaacgtcg ggtaggcttg cacgccctgc ggcacgtaca gcttggtttc gccgaggtcg
      121 cggatgcgcg gcgcgaagcg gaagcctagc aggtgcatca gggcaaagac gtgatcggtg
      181 aagccggccg tgtcggtgta gtgctcctcg atccgcaggt cggactcgtg gtacagcagg
      241 ccgtcgagca cataggtgga atcgcggacg ccgacattca ccacgcgggt gctgaacggc
      301 gcgtactggt cggagatatg ggtatagaac agccgtcccg gctcgctacc gtacttcggg
      361 ttgacgtgcc cggtgctctc gccccggcca cccgcgcgga agcgctggcc atcggaggat
      421 gaggtcgtgc cgtcgcccca gtgggcggca aaggcgtggc gatactggtg gttgaccagc
      481 tcggccaagg ccgccgaata ggtttcgtcg cggatgtgcc aggcttgcag ccaggacagc
      541 ttggcgtagg tcaggccggg gctcgactcg gccatcttgg tcagcccgag gttgatcgca
      601 tcaccgagga ttgcggacag cagcaacgtc ctgtctttgg cctcggcccc gtccttcaag
      661 tgggtgaagt ggcggctgaa gcccgtccag tcgtccacgt ccatcagcag ttcggtgatc
      721 ttgatgcgcg gcagtaactg gctggtttgg tcgatcagcg cctgcgcccg atccggcacc
      781 gccgcatcca gcggggtgat tttcagccct gactcggtga ggatggcatc gggcagctcg
      841 ttgtccttgg ccaggcgggt gacggtggcc aactgctcgt ccagcagctg caaacgctct
      901 tccaggtact ggtcgctgtt cgggttgatc gccaggggca gggcctgctc gcgcttgagt
      961 gcggcgaact tctcggccgg cagcaggtag tcgtcgaagt cgcggaactg ccgcgagccc
     1021 ttgacccaga tgtcgccgga gcgcagggcg ttcttcagct cggacagggc gcagatttcg
     1081 tagaatttcc ggtcgaggcc ttccggggtg atcaccagcg gcttccagcg cggcttgatg
     1141 aaggccgtgg gtgcatcggc cggcaccttg cgcaggttgt cggcgttcat ctcacgcagg
     1201 gtctgcacgg ctgccagcac gccttgcgcg gccggcgcgg cgcgcagttc cagcacctcc
     1261 agcaaggccg gcgtgtaacg gcgcagggtg gcgaagttct cgccgaccag gtgcaggtgg
     1321 tcgaagcctt ccggccgggc cagcagctcg gcctcgctga cgctctcggt gaactcgtcc
     1381 cagggaatca ccgcctcgat ggcggcatag gggtcgctgc cgctttcctt cgcttccagc
     1441 agcgcctggc cgatcctgga gtacaggcgc accttgtcgt tgatcgcctt gccctgcttc
     1501 tggaactgct gctgatgctt gtgcttcgcg ccgctgaaca gcttgaccag gatgcggtca
     1561 tgcagatcga ccagctcatc gatcacggtc gcggtgctct ccagcaccac ggcggccagg
     1621 gtcgcgtagc ggcgctgcgg ctcgaactta ccgaggtctt tgggcgtcat ctgcccaccc
     1681 tcgcgggcca gcttgagcag gcggttctgg tggatgtgcc ggcccaggcc ttcgggcaag
     1741 tccaccaact gaaatgtctt cagccgctcg atgtgttcca gcatgtgccg agagttgggt
     1801 ttcagcggtg cctggcgcag ccaggtcaac caggtgatgc tgctgccggc cttgagcttc
     1861 aacagctcgt ccagcttggc ccgatgcgag tccgtgagtg gttcgaccag ggcgcggtag
     1921 acccgccgat tggctcgcgc aatggcttcc gagcaggccc ggtcaatcac gctcagcgcc
     1981 ggcaggatgc gtcgtttctg ccgtaggctc tccagggcct gaccggccag cagcaagcct
     2041 ttgtcggtct gctgggccag ctcggttagc tcgcgcacca gggcgcggaa gtcggacagg
     2101 ccgaacgggg ccagttgcag gtaggtgcgc agttcctggg catgctcgcg acgggtcacg
     2161 tcgcgctcgc cgtactttgc ccagctcgcc ggctcggcct ggacttgctt cgccacccac
     2221 aggatgaccg gctcgggcag ctcgctgtcg gttcccagcg cgtaaccggg gtagcgcagc
     2281 aggcagagct gcacggcgaa gccgaggcgg ttggcgtcgc cgcgtcgctg gcggatcagc
     2341 gacaggtcgg agtcgttgaa ggtgtagtag cggatcaggt catcctggct ttccggcagc
     2401 gcaagcaggg tgtcccgctc cgtggccgag aggatcaagc gacgcggcat gtgtcagtcg
     2461 tccgtgcgga ggtactggta gagggtttcc cggctgatgt tgaactcgcg ggcaagctgc
     2521 gccttgggct cgccggccgt cgctcgctgc cgcagggtag cagcctgctc atcggacagg
     2581 gctttcttgc ggccccggta cgcgccacgc tgcttggcca aggcgatgcc ctcacgctgc
     2641 cgctcgcgga tcagggcgcg ctcgaactca gcgaaggccc ccatcaccga cagcatcagg
     2701 ttggccatcg gcgagtcctc gccagtgaac accaggccct ccttcaggaa ctcgatgcgc
     2761 acgccgcgct gagtcagctt ctgtaccaag cgacgcaggt catcgaggtt gcgggccagc
     2821 cgatccatgc tgtgcaccac cactgtatcg ccttcgcgga cgaagctcag cagcgcttcg
     2881 agctgggggc gctgggtgtc cttgcccgat gccttgtcgg tgaacacctt gctcacctgg
     2941 gtttgttcca gctggcgttc cgggttctgg tcaaagctgc tgacccggac gtagccgatg
     3001 cggtgcccct gcacgatgtc tccttggttg aaggcggctt aagtgcactt tctgttccgt
     3061 tgtgcctcaa agcccatttc tgtcaggctg aaatctataa ccttcgcggg catgtgtcaa
     3121 aaaatgggaa agcagactct attctgacga agcggcgcgg ccctgcctga catcaagtta
     3181 gggtatgcct caacctgacg gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt
     3241 cgcagtgctg gcccgcgcct ggaagcgctg atagcactcc agcccgcaga aatgctccac
     3301 gtactcggcc ccttccggcg tgaaggcggc atcgagcggg atttccttgc agcacacgca
     3361 gcaactggtg ctcggttcat tggcgttcat ggtggtgttc ctccattggt tgacgaagcc
     3421 gacgaaggcc gccgccggca tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc
     3481 cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca ccctcggcta ccacctccat
     3541 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
     3601 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
     3661 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
     3721 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
     3781 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
     3841 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
     3901 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
     3961 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
     4021 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
     4081 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
     4141 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
     4201 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
     4261 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
     4321 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
     4381 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
     4441 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
     4501 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     4561 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     4621 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaaactg taatgcaagt
     4681 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     4741 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     4801 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     4861 ttacgcagca gggcagtcgc cctaaaacaa agttagccat taagggagtt aaattgaaaa
     4921 tatcattgat ttctgcagtg tcagaaaatg gcgtaatcgg tagtggtcct gatatcccgt
     4981 ggtcagtaaa aggtgagcaa ctactcttta aagcgctcac atataatcaa tggctccttg
     5041 tcggaagaaa aacatttgac tctatgggtg ttcttccaaa tcgcaaatat gcagtagtgt
     5101 caaagaacgg aatttcaagc tcaaatgaaa acgtcctagt ttttccttca atagaaaatg
     5161 ctttgaaaga gctatcaaaa gttacagatc atgtatatgt ctctggcggg ggtcaaatct
     5221 ataatagcct tattgaaaaa gcagatataa ttcatttgtc tactgttcac gttgaagtcg
     5281 aaggtgatat caaattccct ataatgcctg agaatttcaa tttggttttt gaacagtttt
     5341 ttatgtctaa tataaattat acataccaga tttggaaaaa aggctaacaa tgcgttgcag
     5401 caccagtcgc ttcgctcctt ggacagcttt taagtcgcgt ctttgtggtt ttgctgcgca
     5461 aaagtattcc acaaagccgc aacttaaaag ctgccgctga acttaacgtt aggcatcatg
     5521 ggtgaatttt tccctgcaca agttttcaag cagctgtccc acgctcgcgc ggtgatcgag
     5581 cgccatctgg ctgcgacact ggacacaatc cacctgttcg gatctgcgat cgatggaggg
     5641 ctgaagccgg acagcgacat agacttgctc gtgaccgtca gcgccgcacc taacgattcg
     5701 ctccggcagg cgctaatgct cgatttgctg aaagtctcat caccgccagg cgatggcgga
     5761 acatggcgac cgctggagct aactgttgtc gctcgaagcg aagtagtgcc ttggcgctat
     5821 ccggcgcggc gtgagcttca gttcggtgag tggctccgcc acgacatcct ttccggaacg
     5881 ttcgagcctg ccgttctgga tcacgatctt gcgattttgc tgaccaaggc gaggcaacac
     5941 agccttgcgc ttctaggccc atccgcagcc acgtttttcg agccggtgcc gaaggagcat
     6001 ttctccaagg cgcttttcga cactattgcc cagtggaatg cagagtcgga ttggaagggt
     6061 gacgagcgga acgtcgttct tgctcttgct cgcatttggt acagcgcttc aactggtctc
     6121 attgctccta aggacgttgc tgccgcatgg gtatcggagc gtttgcctgc cgagcatcgg
     6181 cccctcatct gcaaggcacg cgcggcgtac ctgggtagcg aggacgacga cctagcaatg
     6241 cgcgtcgaag agacggccgc gttcgttcga tatgccaaag caacgattga gagaatcttg
     6301 cgttgagcgg catgtgcgaa aagtgcatcg acccgcgccg agggcatctg atgcctaact
     6361 cggcgttcaa gcggacgggc tgcgcccgcc gctcaactat gcgttagatg cactaagcac
     6421 ataattgctc acagccaaac tatcggcact gttgcaaata gtcggtggtg ataaacttat
     6481 catccccttt tgctgatgga gctgcacatg aacccattca aaggccggca ttttcagcgt
     6541 gacatcattc tgtgggccgt acgctggtac tgcaaatacg gcatcagtta ccgtgagctg
     6601 caggagatgc tggctgaacg cggagtgaat gtcgatcact ccacgattta ccgctgggtt
     6661 cagcgttatg cgcctgaaat ggaaaaacgg ctgcgctggt actggcgtaa cccttccgat
     6721 ctttgcccgt ggcacatgga tgaaacctac gtgaaggtca atggccgctg ggcgtatctg
     6781 taccgggccg tcgacagccg gggccgcact gtcgattttt atctctcctc ccgtcgtaac
     6841 agcaaagctg cataccggtt tctgggtaaa atcctcaaca acgtgaagaa gtggcagatc
     6901 ccgcgattca tcaacacgga taaagcgccc gcctatggtc gcgcgcttgc tctgctcaaa
     6961 cgcgaaggcc ggtgcccgtc tgacgttgaa caccgacaga ttaagtaccg gaacaacgtg
     7021 attgaatgcg atcatggcaa actgaaacgg ataatcggcg ccacgctggg atttaaatcc
     7081 atgaagacgg cttacgccac catcaaaggt attgaggtga tgcgtgcact acgcaaaggc
     7141 caggcctcag cattttatta tggtgatccc ctgggcgaaa tgcgcctggt aagcagagtt
     7201 tttgaaatgt aaggcctttg aataagacaa aaggctgcct catcgctaac tttgcaacag
     7261 tgccgttaaa agcaacaaaa atgacttcgt tgatgctgaa gctatctgtg aagcagcatc
     7321 acgtccatct atgcgtttcg tgcagcccag aaccgaatct cagcaggcaa tgcgagctct
     7381 gcatcgtgtc cgtgaatccc tggttcagga taaggtgaaa acaactaatc agatgcatgc
     7441 ttttctgctg gaatttggta tcagcgttcc gcgaggtgct gccgttatta gtcgactgag
     7501 tacccttctt gaggacagta gtttgcctct ttatctcagc cagttactgc tgaaattaca
     7561 acagcattat cactatcttg ttgagcagat taaagatctg gaatctcagt tgaaacgaaa
     7621 gttggacgaa gatgaggttg gacagcgctt gctgagtatt ccctgcgttg gaacgctgac
     7681 tgccagtact atttcaactg agattggcga cgggaagcag tacgccagca gccgtgactt
     7741 tgcggcggca acagggctgg taccccgaca gtacagcacg ggaggtcgga cgacattgtt
     7801 agggattagc aagcggggca acaaaaagat ccgaactttg ttggttcagt gtgccagggt
     7861 attcatacaa aaactggaac accagtctgg caagttggcc gactgggtca gggagttgtt
     7921 gtgtcggaaa agcaactttg tcgtcacctg tgctctggca aacaagctgg ccagaatagc
     7981 ctgggcactg acggcgcgac agcaaactta cgaagcataa aggcagaaat acaccagttt
     8041 aaacaatcat tcatctggtt ttgcgaatac tgatattgat gatactaacg gcccaccggc
     8101 ctgttgagga acctgtaaaa cggaaaggct cattgaagcc gtatattttc tggaggttca
     8161 tcaggcgcgg aactcatcga ggcgcgggaa taaaatccca ttcagacgcc ggatagattc
     8221 aagcaagcca acttgtcgtc aaaatcggtg ttgcaaaaac gggagtgacc atagattccg
     8281 ttttctgagg cgacccccac atagagacct tcccggtcat tcactttgta gagtttgtcc
     8341 ctgggcttga gattgcgcag cttggtatcg gtcagcatgt accttgtcct tcttcgtctc
     8401 cgatacccac tctattgtaa acaagacatt tttatctttt atattcaatg gcttattttc
     8461 ctgctaattg gtaataccat gaaaaatacc atgctcagaa aaggcttaac aatattttga
     8521 aaaattgcct actgagcgct gccgcacagc tccataggcc gctttcctgg ctttgcttcc
     8581 agatgtatgc tattctgctc ctgcagctaa tggatcaccg caaacaggtt actcgcctgg
     8641 ggattccctt tcgacccgag catccgtatg agactcatgc tcgattatta ttattataga
     8701 agcccccatg aataaatcgc tcatcatttt cggcatcgtc aacataacct cggacagttt
     8761 ctccgatgga ggccggtatc tggcgccaga cgcagccatt gcgcaggcgc gtaagctgat
     8821 ggccgagggg gcagatgtga tcgacctcgg tccggcatcc agcaatcccg acgccgcgcc
     8881 tgtttcgtcc gacacagaaa tcgcgcgtat cgcgccggtg ctggacgcgc tcaaggcaga
     8941 tggcattccc gtctcgctcg acagttatca acccgcgacg caagcctatg ccttgtcgcg
     9001 tggtgtggcc tatctcaatg atattcgcgg ttttccagac gctgcgttct atccgcaatt
     9061 ggcgaaatca tctgccaaac tcgtcgttat gcattcggtg caagacgggc aggcagatcg
     9121 gcgcgaggca cccgctggcg acatcatgga tcacattgcg gcgttctttg acgcgcgcat
     9181 cgcggcgctg acgggtgccg gtatcaaacg caaccgcctt gtccttgatc ccggcatggg
     9241 gttttttctg ggggctgctc ccgaaacctc gctctcggtg ctggcgcggt tcgatgaatt
     9301 gcggctgcgc ttcgatttgc cggtgcttct gtctgtttcg cgcaaatcct ttctgcgcgc
     9361 gctcacaggc cgtggtccgg gggatgtcgg ggccgcgaca ctcgctgcag agcttgccgc
     9421 cgccgcaggt ggagctgact tcatccgcac acacgagccg cgccccttgc gcgacgggct
     9481 ggcggtattg gcggcgctga aagaaaccgc aagaattcgt taactgcaca ttcgggatat
     9541 ttctctatat tcgcggttca gcaggcatgt cccctttgag ggcgacccga cgacaggata
     9601 atcgacctta tggtgcgcaa atatttcggc acagacggta ttcgtggcaa agccaacgaa
     9661 ggcgcgatga cggcggaaac cgccttgcgc gtcggcatgg cggctggccg tgtctttcgt
     9721 cgcggtgacc accgccatcg tgtcgtgatc ggcaaggata cgcgcctgtc gggctatatg
     9781 cttgaacccg cgctcacagc cggtttcacc tcgatgggca tggacgtatt cctttttggc
     9841 ccgctgccga caacgtatag gaagaataaa cgcccttttc acccaagtcc aacagctttg
     9901 gaccgcagtt gactctttcg acacccctgc gatgcaaccc aatccggctg acggggagcc
     9961 agcaacgctg aaaatttacc ctcctctttc ccactagcgg ctccttttcc gacaaccagc
    10021 acggcggatc cctgccgcgg cgctgtgaac gcagcatttt gattggtatc gttggccttc
    10081 aggctcgtca gtcaaacaga cccaggagca gctcagccgg tggcgcccgg ctttcgggta
    10141 acgccctggt cccgctggtt tcggctttgt gcttcaggtg atcaaggatc tgcttgatca
    10201 ctatagggtc ttcaatgcag gcgatgactt tcatggcgcc gccgcagccg ctgcaggtct
    10261 cgatgtcgat attgaaaaca cgcttgagcc gttgcgccca tgtcatcgac gctcgccgtt
    10321 gtgctggtgt tgccggttca tcagccaccc tgaccttgtt gcccctgccc cgttttgccg
    10381 gcgtgaccaa cgcccggtgc cgactgttgg gtgcgaacac cccgtggaag cgggttaggt
    10441 tgactctggg cttcggtacc agggcggcca gccttgcaat gaaatccaat ggttcgaaaa
    10501 tgacgtgcgt ggtgccgtcc cggtacggcg tcttgagctg gtagcgcacg ttgccgcctc
    10561 gtgttaacga cagccgcttc tcggataccg ccgggcggct gatgtaccgg cacagccgtt
    10621 cgagcttctt gcgttcatcg gccctggccg ccacgccggc gtgcaggctg gacccggcta
    10681 ccttgccaat cccgtcaccg aacggatcac cactggtcgg cagagtttgc aaagtgaaca
    10741 cctttcgccc cgcctgtgaa ccgacagcga tacggtaagt gatcgagtgc cccagcaggg
    10801 gtgtcatcgg gtcgtcatcc accgcatccg aggccagata gctgttttcg acatcccgtt
    10861 ccagcaggcc ttgccgttcc agatagcgac ccacccggtg ggcgatggtg tgcgtcagct
    10921 gggtgagctc tggggtggtc ggcgccttga cccagcggaa acgcgctgag ccgtgggatt
    10981 gctcgacata cacaccgtcg agaaacagca tgtggaagtg aacattcaga ttgagcgccg
    11041 atccaaaacg ctggatcagg gtgaccgcgc ccgtcttggc cacttggtgg gtatggcccg
    11101 ctttcttgac caggtgcgtg gcaatgacgc ggtaaacgat gcccagcacc caccccatga
    11161 tctcgggccg gctggcaaac aggaaacgca gctgaaacgg gaagctcaac acccactgac
    11221 gcatgggttg ttcgtcctgg acggcgaacc cggcaaagct cctgtaacat tgcttcttcc
    11281 tgttttgtta gcgtgactgt cacgctagcg ccttcctctt tgagtcgcgc gcggcgccgg
    11341 gcctggcgaa tacgggcttg ttcacgctgt cggtcattca tatttttcct ccacgtgact
    11401 gtcacgttga attaaactgg gcattacccc tgcggggccg ttctgccgtg ctgcgcatcg
    11461 agccgctacg cgtctcgccc cttcgggcgg cggccactaa tgcctccggc ctgcggcctc
    11521 cgcgcttcgc ttgtgacgat atctgaatcg ttatatactg tgtatatacg tagtaatgac
    11581 gaggtgataa atggcacagg ttaatatgag tttaagaatc gacgctgaac tgaaggatgc
    11641 ttttatggcc gctgcaaaaa gcatggaccg taatggctct cagttaattc gggattttat
    11701 gcgccagacc gttgaacggc agcataatac ctggttccgt gaccaggttg aggcaggacg
    11761 tcagcaactc gagcgcggcg atgtgcttcc ccatgacatg gtcgaatctt ccgctgctgc
    11821 atggcgggat gaaatgagca ggaaggttgc cggtaaatga tggagatatt ctggaccatg
    11881 ctggccagcc aggacaggaa gcgcattcgt aagtacattg cggagcagaa cctgatggcc
    11941 gccattgaac tggacgaacg gattggttat tcagccagca gcctggccgg gcagccgtat
    12001 aaaggccgca acggtcgtgt ggaaggtacc cgggaactgg ttattcaccc gcattttgtt
    12061 ctggtttatg aggtcgacag ccagtgggga aaagtgtata tcctgcgcgt gttgcatact
    12121 gcacagaagt ggccatagag gggctgtccg gatataagga aaggagatgc aataaccatg
    12181 aataatgatg agctggccac cagacgtgcg caggcgattg ccgaagacag gtgtttctcg
    12241 aaaggacgtt tgcgcgatga attcagaatg aaaccagccc ccggtgctga gccggttaag
    12301 tggtataaaa atagttatgg tggcaggttc gctgtatacc gaattgcaga ctgtgttccg
    12361 atgcgtgaga aacgccctct gacgagtaaa caacagctgg ctggccagcg gctttccgtt
    12421 ttatcccgac taaacagcac cagtggccgg atggcacggc aggcatatga ctggctgagt
    12481 ctggcaccac tctttctgga tactgaaacg acaggactgg ataataccgc ggaagccctg
    12541 gaaatcggcc tgaccgatgc ggccggtcag gtggtatttg aaaccaggct gaagcccacg
    12601 gtcgccatcg gggcgcaggc cgccgccgtg catggtatca gtgagcaggc tctctgcggc
    12661 gcaccatcat ggacagatgt ggcgcggcaa ctgcgtcatg caatcgggga ccgaccagta
    12721 attatcttta atgcccggtt cgacatccgc attctgaaac agactgctgc cgcacatagc
    12781 gatccggctg actggctgga agaactgacg gtatattgtg cgatggagct ggctgcagga
    12841 tattatgggg ccaccaaccg ctatggcacg atttcactgg cctgtgctgc cagtcaggcc
    12901 ggactgacct gggaagggca ggcacactca gcgatcgccg acgcacggat gacggcaggg
    12961 gtggtaaacg ctattgctgc atatcatctg gaactgctgc aggaacaggc acggctgaaa
    13021 atctgaccgc ctggcctgta taccgcaatc atttacgtta tccagacgta aatccgccgc
    13081 ttgttgctgt cagcatgtta atgctaatgt atgtgtacat tagcattaat gagggatgat
    13141 atgtgagaga caaaaccagg gaggccatgc ggcttttttt aggcggcagg tgctacacgg
    13201 ctgaaaaact tgaaaaggat tatctggctg aagtggctaa ttacagtaac gaccgctggg
    13261 aagcaccgca acgtgcctca cgcctggctg ccagcgtaaa gcgttacaag acatcagaaa
    13321 tgctgcgctt tatttttgct accattgcct acgatcccga tccagattta accccgctaa
    13381 ctgtgagacg tttgtgcaag gcattgttcg gcagaaccgg aagccagtgg ctggttgttg
    13441 aagtttttgg ggagaagggg cgtcagcacc gcagcgcgga cagcaatccg gagatggtgg
    13501 aaaaaatggc tgccagatat cgtcatgccg ccgaactaca ctggtccgca acgctggcag
    13561 aaattgaacg ggtaaagcgg ctttatcaga cgaaaattaa gaaatccaaa aaataagtgg
    13621 gttgactgcc attgttgttc ttctccttca tcattaatgt taatgtatgc atacattagc
    13681 attaatgatg gtgcgttaaa gtatcactgg tataatctgg taaagaacat attgagaatg
    13741 agaacttatc atgtcgtcac gcaactctgt acatgatttt ataatgctgc tcccggcgct
    13801 gctgttggtt gggtggttag ttttggtgat aattctcatg tttagggatg ataaatgtga
    13861 caaaacttac cgtgaaggtt cgtttcccga atttatttgt ctccattctg atcgctcgtc
    13921 acttcgagaa accgattctg agagactcat tgtcaaatat acagaaataa aatcacaaaa
    13981 ataggtaaaa ccaataatac tatagctaaa aaatagttac ttttctgtat caccctgtct
    14041 ctctctgctt tttcatggct actgtcatat ttatgtctgt tgcgcggttt tgttactttt
    14101 tcatgaccga acccgaaatt ttgcgcgtca ataagagacg ttgtgtaaac tcagtccttt
    14161 tatctggcag gttaacccct atcaatctta ggatgaaacc acgccatcct ctgtttgcct
    14221 ggttattaat ttcataaaga cgattgtttt cctctcgtag ttttgtccac tctaaacaaa
    14281 gtgttatatc atccagttct gtgatgtgtt tcacttttcc attgacagtt acagtgttta
    14341 ttccatttcc tgtgagcatc atcttatgcc ttttttatct tcatttggga ccgtgctttt
    14401 cttcggagta tcactattcg ttaactgctg tttatcaata aaattcctga tgttatcaag
    14461 tgcttccttt gtttttagaa tgcgcttgat atccatttca tcccgcccgg tcattctttt
    14521 tccggacaaa agacgctcaa gaagctcctg tttcgcatca ggagaatctt tggcgagatc
    14581 catcaggtta agcaaatctt cacgatcgag aggaaagaag tattttacaa aatacttgaa
    14641 aagcgaaaga aagtagtatg ctgtccatgc aatacagaaa ccgattctga gaaactcatt
    14701 gtcaactata cagaaataca ggtatacaat caggagaata ggtaaaatcc ataatactat
    14761 agctaaaaaa atatatacgg ggatcaggcg tctgtatgcc cgttcatgtt tattcaggat
    14821 gaataaaatc ttctccgctt ctgaatcaca cataacattc tcttcccatt ttttctgata
    14881 cgtaatgtcc acctgttatc aggagtgttt attgcccaga aatattctcc tgctgtgcct
    14941 gcgtcctgag ccacttttgt tctaatgtcg ccagtctgcg accggcactc tgtgcttcag
    15001 agtctgagac ctgccctttg gggtgaccat ccaggccata acgtgggacg ccggcgacag
    15061 ctgtccgctg cagatatacg cggcgggatg tgatgcagga cactgcacag agccattcgt
    15121 ccatggtcag ttctgtctcc gggtgttctg ccagaaatgc cgaaatatca ttgcttatgc
    15181 caattttcag gggaataaat ccaccacctt cagcacgaag atgaggaaac agggccttta
    15241 ttttttccac gcactcgtga gtatactcac gggtccagtg ggagggacgt ggaaaacggt
    15301 gtcttttctt tttcttcttc ctggctggcg cttcggggat atgtgtttcg acggcagatg
    15361 aatttattcc ggcgggggct gattctgccg tctgttcagt aaatacaggt gcgataatat
    15421 ctgttttttc ggataaggac ggtggcgaaa aagtacgacg ttttttcacc acaatgacgg
    15481 gagttttcct ttcttcctga ttttttccca taaatacctc ggctggctgg ttttactctt
    15541 taataatatc acaatccata aattagtaaa tttcccatca ggtgattttt catggctaat
    15601 aagtccattc tctgaatatt tatactcgag gtagttacag ttatggcgtg acagtcgaat
    15661 tgatattcta ctgaaccctg acagaaacat ggttgatata ttctctcact ttaatgttgt
    15721 catgatataa ataaaaacaa ttcatttgaa aggatatcag aagaagatat cggggcgcac
    15781 agttcatctc tgcctttgtt tcatgggggg ttctttgtat attttactta ctgtttaagc
    15841 atgtacataa ggagtgcttt cattatgcca atgatacttg taaagaagaa tccccggggc
    15901 aaagtgattc gggagcttag cagtgaggaa gagactgcag taaagacggt ctgtggactg
    15961 aaaaggccag caaccatggc acaacataac ctggccaatg atcttctccg ggaaatgcgt
    16021 gagtatgatg catggttaca gtgtgactgt attccgggag attcgccggc catgaatttt
    16081 gctgctctta aaaacaatac gggtactctt tatctgtcca gttttaatca tgagcatgca
    16141 cctgaatgcc ccatgtatcg tcagttatcc ggaaatgaag aggaaacatc cttcggagca
    16201 agccggcatc ccgttagcac ccgtataaat tacagaaact ttttaccacc tgatgacagt
    16261 aattctgtta tcagactgca ggcccgctct gcttaccata atggtgagcg ctcctccgtt
    16321 cgcaagaaac gtccgcgcct tggccgttta ctcctgtcgc ttattgaaga tgcagggctg
    16381 aataaactgg attccctggc taatccccgt atccggacta attatcgtga gtgtcttgat
    16441 gccatacggc aggttacact acagcaggag tatattcggg gaagagcctt atctgaaatt
    16501 atccatttcc ggccaggaat gtctgaaagg tctcaggagc ggttaatgga aacgctggag
    16561 aattcagaac gacactggcc tgccagaaga aagcatatgt tctttcagat ttttatggcg
    16621 cagcacatct gtcgtgatgc tgtggaaatt cactgggcga acggtaatat tcaggtcatc
    16681 agacctgtgc gggggatcag tattaatggt gaagcgcagg gcggaatacg tcccccttac
    16741 tgggttattc ttgctttttg ccggagtgcc gatggcagaa tcatctgcag tgaagggtat
    16801 gctcatgctc tttatcaact gacatgtccg gtgcctgtgg acagcaaact ggaacgaaac
    16861 acgctcactg ctcttctgaa tgtggccagc tggcttaaaa gaaagccagg tacgccggaa
    16921 ttaagtctgg aaaggcccct gtttgataca gaagtttatg ttaatggtga aaagaaatat
    16981 gtactgccgg atttcattgt cacagcaagg gctcctgacg gaaagacggc cagagtggtc
    17041 atcgaaacga tgggatatga agacagtgat tactgcgcga gaaaatccag gcagcatacc
    17101 ggcatgaagc agattggtgt tctgcatacc gatccaccga aatggctgga taacgatcat
    17161 ccccctttta agaaacatat gtacggtgtt tttatgcatc tcaggtactg agatattttg
    17221 tggctcagtt ctgtaacttt tcccgtaaca ttgtctgttg ttacgggaaa gtccggtttt
    17281 tgtattgcac cagagaatac ccagactgtg atgctgccac agtgtcagca ggctttctga
    17341 acggtataac accacttcat gttaatgata attactatca ttaaatcttg acatgccatt
    17401 ttctccttaa taaattagta ctgtatatgt atccatatac gtaagcagtt aattcatttg
    17461 ttttcctcag aggatgaagg agataccgaa tgtctgaccc tgtacgtatt acaaatcccg
    17521 gtgcagaatc gctgggatat gattcagatg gccatgaaat tatggccgtt gatatttatg
    17581 taaaccctcc acgtgtcgat gtctttcatg gtaccccgcc tgcatggagt tccttcggga
    17641 acaaaaccat ctggggtgga aacgagtggg tcgatgattc cccaacccga agtgatatcg
    17701 aaaaaaggga caaggaaatc acagcgtaca aaaacacgct cagcgcgcag cagaaagaga
    17761 atgagaataa gcgtactgaa gctggaaaac gcctttctgc ggcaattgct gcaagggaaa
    17821 aagatgaaaa cacactgaaa acactccgtg ccggaaacgc agatgccgct gatattacac
    17881 gacaggagtt cagactcctg caggcagagc tgagagaata cggattccgt actgaaatcg
    17941 ccggatatga tgccctccgg ctgcatacag agagccggat gctgtttgct gatgctgatt
    18001 ctcttcgtat atctccccgc gaggccaggt cgttaatcga acaggctgaa aaacggcaga
    18061 aggatgcgca gaacgcagac aagaaggccg ctgatatgct tgctgaatac gagcgcagaa
    18121 aaggtattct ggacacgcgg ttgtcagagc tggaaaaaaa tggcggggca gcccttgccg
    18181 ttcttgatgc acaacaggcc cgtctgctcg ggcagcagac acggaatgac agggccattt
    18241 cagaggcccg gaataaactc agttcggtga cggaatcgct taagacggcc cgtaatgcat
    18301 taaccagagc tgaacaacag ctgacgcaac agaaaaacac gcctgacggc aaaacgatag
    18361 tttcccctga aaaattcccg gggcgttcat caacaaatca ttctattgtt gtgagtggtg
    18421 atccgaggtt tgccggtacg ataaaaatca caaccagcgc ggtcatcgat aaccgtgcaa
    18481 acctgaatta tcttctgacc cattccggtc tggactataa acgcaatatt ctgaatgacc
    18541 ggaatccggt ggtgacagag gatgtggaag gtgacaagaa aatttataat gctgaagttg
    18601 ctgaatggga taagttacgg caacgattgc ttgatgccag aaataaaatc acctctgctg
    18661 aatctgcgat aaattcggcg agaaataacg tcagtgccag aacaaatgaa caaaagcatg
    18721 caaatgacgc tcttaatgcc ctgttgaagg aaaaagagaa tatccgtagc cagcttgctg
    18781 acatcaatca gaaaatagct gaagagaaaa gaaaaaggga tgaaataaat atggtaaagg
    18841 atgccataaa actcacctct gatttctaca gaacgatata tgatgagttc ggtaaacaag
    18901 catccgaact tgctaaggag ctggcttctg tatctcaagg gaaacagatt aagagtgtgg
    18961 atgatgcact gaacgctttt gataaattcc gtaataatct gaacaagaaa tataacatac
    19021 aagatcgcat ggccatttct aaagccctgg aagctattaa tcaggtccat atggcggaga
    19081 attttaagct gttcagtaag gcatttggtt ttaccggaaa agttattgat cgttatgatg
    19141 ttgctgtgga gttacaaaag gctgtaaaaa cggacaactg gcgtccattt tttgtaaaac
    19201 ttgaatcact ggcagcagga agagctgctt cagcagttac agcatgggcg ttttccgtca
    19261 tgctgggaac ccctgtaggt attctgggtt ttgcaattat tatggcggct gtgagtgcgc
    19321 ttgttaatga taagtttatt gagcaggtca ataaacttat tggtatctga cacataatga
    19381 tgccctttca ttttcctgaa agggctttgc agagtataaa aattaagtaa atggcaacca
    19441 caggaactga tataacgaaa cagaataatt caaatattgc agttaagctt ccgccaactg
    19501 gatttgtaaa gaagtctttt tgccagaatt ctttccctgt aaaccttatt accattttct
    19561 gtattatcct cattgaaaaa ggaaaagtaa tggcactgat aatgcatccg atgaacgcat
    19621 aaaacattct tgcattttcc tggttatctt tccatcccag ataaaaaact gtaagaataa
    19681 tgaggattgg tatcaggctt ttcagtaaat actttacaga tatatccagt ttcatctggt
    19741 ttctccttta ttgttatcgt gtaccggtgc gtgataacat aatcacagca tgataataat
    19801 aatcaataac aataagctgt gtcacgttta catcatggaa cggaggtgct cctgtgacct
    19861 ttgagcagaa aaaagctcgg gccattgccc tgatggacag caaaaagatg tggcggagca
    19921 attatgcccc tcctcttctg cgtattctct ggcggctggg tatccggctt ccgccattac
    19981 cctttatgcc gttctggcag gtcacggtgc tgacgggggg cttatggggc atttcctggg
    20041 gatgtgcaat gtggtttatc tactgggggc cgtcaggaat ggtggcaggt gaggccatta
    20101 ttataagtat cacggggggg ggttttgtcc ggactgtgca tggcctcttt ccactggtgg
    20161 cgcaggaaag ttaaccggct gccaccatgg gatgatgtgt aaaccgtgca caggcgtgtt
    20221 cttacggagc ggcactttca tttatctgta cgcgaacctc cattcctgca tcgtgacagg
    20281 cctgcagcca ctgcgccact tccagcggat cgccctcccg gcgtaccacc ctgccttctt
    20341 tattccataa ctgcagacag gtgctgccgt ctagacgcac cacaaaatcc ccacggcagg
    20401 cctgatacgt tatgccgttc agttgcaccg ccagatcagt ggcagaagtg ccatctgcgg
    20461 gcagacctgc tgccgggacc agcggcagta cgacgacttc tgccgcacgc tgttctgccc
    20521 ggaggcggtc ctgttctgcc agcagcagag gctgtgccac ggcccggccg gcgtccgtca
    20581 gctctactgc cagctgcagg ttcggagcgc gcagggtacg cagccagccg gcatcctcca
    20641 gacgacggca ggaggcgcgc aggtttggcc cgtagaccgg ggcttccccg cttcgctcca
    20701 gcacccgttc tatgtcccgc gtcagcacgg gtcctggccg tttttcatca agggcagcca
    20761 gcacaatcag cactctccgc tgcagtggcg acggtcggcg gctctgggtc attacctgtc
    20821 atcctccggc cagatgaggt ttctgaggcg ctgaaagtcg gcgtcaatga gctcctgtct
    20881 ctttatggca ttcattgagg catagccatt tatggccagt tcatgccaga agatcagcac
    20941 ggctccggct ttggctgact cgcagagata aatcgcgccc tggctgtcca gattgcattc
    21001 ccggatgcgt cgttccgcaa accgggtcag ttgttcatag ctgagtgaaa acgtcatttc
    21061 ctgatccatc ctgcacgctc catctgataa tattctgaca ggtaataatc atatcctgaa
    21121 tgatttgtgt gctcctgatt tctgataata tcagatatgg ttttatcatc aatgatgaaa
    21181 atcatatctg atatgatttt atgtgtattt tttattatca tatcatttgc gtcttttctg
    21241 ccgcgcagat ccggtttccg gcggttccgg agatttaaat tccatcggta ctgacagggc
    21301 gttaaacaca ggtattatcg aacggttctg ttttctaatg ctaagcttca aaccagctgc
    21361 ccatgccgtc aatacagatg caccgatgcg cttgcgtggc ttttcaggga gataaagtta
    21421 tggccgatcc attcaatctg cagaccgatg tggtcaggca gcatacggtt ccccgttttt
    21481 tactgaagca tttcagtacg ccgggaaagg gtaagcggca gcgcttatac gcctttgaca
    21541 aggctgccgg gcgcgcctat gccaccacgc cggatgacgc gaccgtccgc aacacgttct
    21601 acaacctcga taatcacccg gaccgtctga gtcttgaacc actgctgggg atttacgagc
    21661 accacgcggc accggtaatt gcagccctgc tggcgcacag ggatattcgc aggctgacag
    21721 atgatgaacg ctacaggctg gccgtgtttg ttgccgtgca gcgcgcacga accttcggtg
    21781 aactggagcg tatctcaggc atgatttcgg tgcttaccga taagatggag gccatcggct
    21841 cgactaaaga gcaggccatg gaaaccctgg gcctgtcgtc cgggggcgat accaaggata
    21901 tttttctgcg gcagctcgtc cagcaggtgt cacatattga tctcctgctg aaaaaggact
    21961 ggtaccttct ggaaaccagg ccagaacgtc cgttttacgt atcagacaat cccgtagtgc
    22021 ttaagaacag caatgacttc ggtccgtacg gtaatcttgg tctggccgtt cgcgggatac
    22081 agatttacct ccccctgtca tcgacgctga tgctggccat gtattgcccc tccatccgtg
    22141 aacagatggt tcgtcaaaag cagcatcttc aacatctgct cgccagggcg ccgcatctta
    22201 tcccccgtca cattcggcct tttgaaagac tggaacatat ccggaggtat acggactatc
    22261 ttcttatgcc gcttaccccg gagcatgtca cgcattacaa ttccctgcag gtggagttcg
    22321 cggagcagta cgtgttctgc ggtgaaaagg atttctctct ggttgagaga atgctggccg
    22381 atagtgaacg ctaccggact ggcccgcggt ttacgtttta gtccgaattc cggcctcacc
    22441 tgtgccggaa aatgacggcg ggagcccctg ttgtacagag gagttttgcc gtgttgcacc
    22501 cacgcgaaag cggcagtggt cacagggacc accgccgtaa ggagatggtg agtatctgtt
    22561 gacattcacc atcagttcgt gtagcgttgc ggctacttac tttagcagag taaatcttcg
    22621 gcaagtggcg gtaaccactt taccgatacc ccagagtagt ccgcgcttta ctggcgtgac
    22681 ccatagtgcc gaaaccgcaa atttcgacac tataaagacc ttcagccagg gcactacaaa
    22741 ccagaccacc gcagcacaga gacccacata ggccataaga agcaaaatac agagacaggc
    22801 gctccagtat tgcttctggc aaatatggtc cctggttccg ataaccaggc gtttaacggt
    22861 ggagaaaatc gtccaaataa aatggatttc agtctggaat gggaatagaa ccgttccctc
    22921 ggtgtctaac aagtttttca tagaaccctc ccgggtaagt atttacaaaa gtaactgtct
    22981 tgattttggt catctcaata acctcgtttt taagtcaaaa ggcacccggc gcctcacaca
    23041 cgtgaacgct gttaatgccg tacagaatgt tttatctttc agctctgtta tgatgtgttc
    23101 attcgcatcc tcctgtttgt cataccgatt ttaagctcgt tccgggggct ccccggaaaa
    23161 aaatccaaaa aaatccgggc cggaatggtg ataaccagtc tggcgggaaa taaacggatc
    23221 ttgttggggt ggagcgggac ggaacaatac tatgagagta atgttaacag aaatcaccgc
    23281 ctttgtcaag gcgcacttat ccattaaagc aactatagca tagtaatact gatgtccatt
    23341 tagcaaaaag tgctatccgt cgttctgctg tatgtctatt ttccttcacc tccgggcaac
    23401 ctggcgaata catttttact ttttttgccc cctgccccct cagagtaccg agacgaagag
    23461 atgtgtggcc acgccctgcc ggcgtccggt aaacggcccc tgcgccgtac ccgctgacag
    23521 ccggcatttt cgcccggaac gggtgaaggc tgacttgagc gatttggagg cctcccgcag
    23581 cgcatcctca agaccggtgt tcagacagag cctcaccacc gcccctgaag ggagacgggc
    23641 aatctccgcg aaactctctc cgccggtaac atagcggctc tgtcgtcctt tcattgtatc
    23701 ctcctgatga tgcagttgtg cccgcagatc cgggtccgtt acagcaggtt ttcttccacc
    23761 acgttgatca gcgcctgagc gacatccccg agcggggtgg gattgtgatc ttcttccaca
    23821 ccgttggtgt aaaggtgaag ctcacacacc ccgtccacaa aacggaaggc cttgtcccac
    23881 ctgtcgggtt cggcgctgat cagggcgctg tagcccggat cggcggtaaa gaggcggtat
    23941 tcaagctccg ggaacgggat gttccacatc tgggtggcgt aattttccgg gaggctcagt
    24001 gtaacgcgcc atgcgccgcg ggcgtaattt tccgggcggt agatacgcac cgtgatgcca
    24061 tcccgcgtcc agctgtcgcc ggtgagggca ggcagttttt tcagggcgcg gatggcctcg
    24121 atatcttggt aaaagcggtg ttttttttcc ctcagttgcg agtagaagat atttttccag
    24181 gcctggtcga tggtttccct gtcccggccg gcgatctcgg caaacaggga gaaggtcagg
    24241 gagatcccgc gcgggatatt tttccacccc ttaagcacct ggtcggcacg gacctgcagg
    24301 aagggggtgt tgttcagtac caaccggcgg agctcctctt ccgtcgagat ccccagcagc
    24361 ttctggtatt cctgattcca gcgggtgata aaatggctct ggtagcgctg ctccatgacc
    24421 ttagcgtcca gataactggc cagctcctca tccagggagg cgatgagcgg gttcttcagg
    24481 gcaaagctgt cgatgacgtc gcggaagctg tcttccagga actcacgcag aaacgtcgcc
    24541 tttggcttac gggcgtggct tgccaggtcg gtgatcaccc ggtcggtgtc agcggggatg
    24601 ttccgcaggg tatacgttac gctctctcca ccgtcgtttg tcatgtcatg ttctttttca
    24661 ctcatcccgc gtaccttttc atgtgagggg gcattgctgc aatacttata ctctatcctg
    24721 gttttgagtg tctttaaata ctgcgaagca tacgttttta taattcgtat gcttcgctgt
    24781 ctgttttctg ttcacttcct tttctgagat ccgaaaaccc caggtcacgg atcttcctct
    24841 ccccccacac aacgccacct cctgtaagca caatatgtag tgctgcattc agctgctgat
    24901 gacactatat gttgtgttat ttcccttgcc tgtgatgcgc cgtgcagggg cggaaaacag
    24961 cgatatgatt atttcctcat cgtggtacac ttccggaaag ttgtgatatt ccggaaagtc
    25021 ggatctgacg gaaacggctc tccggtaatt taactgcgtg gttatatgga tgcttgttat
    25081 catggtgatg atgataacgg catgatgtta tcagacggcg tgacggtaag ggcagtgatg
    25141 atggatgacg ttatcgcatg accgtccctg cccggaaaag aaaaaaggag tcacccatgt
    25201 tttttattga gaatgaaggt caggctgtcg ccagaacgga ttactggcag tctgtacagg
    25261 cgcaggccgg atatgtctac ctcagctgga atgccggcgc agccaggctg cttgtcccgg
    25321 atgcggcaaa acatttactc agggagatgc ggggagctga gtacgtcatc atcagtaagg
    25381 gaacgctcca tggccgcgat gcgctggaac tggtatttga agacggcagc gatgcgccgt
    25441 tcgtgatcca catgctgagt gagcagtgcg atcgcctgct ccccgaaaac aaccagggag
    25501 ggggttttgt tgtcaccgtc tggacgcgtg gcggtaacca gctccgttat ccgggaaaat
    25561 accgggttgt ggaaaacctg cccgacgttt ccccgtggag tgaacactga tatgcagcac
    25621 ctgccggcac cgatccacca tgcccgggat gccgctcagc ttcctgttgc catcgattat
    25681 ccggcagcgc tggcactccg ccagatgtcg atggttcatg atgaactgcc caaatacctg
    25741 ctggcccctg aagtgagcgc cctgctccat tacgtcccgg atctgcgccg caagatgctg
    25801 ctggccacac tgtggaacac cggtgcgcgc attaatgaag cactggcgct gacgcggggg
    25861 gatttttcgc tcacgcctcc gtatccgttt gtgcagcttg cgaccctgaa acaacggacc
    25921 gaaaaagccg ccaggacggc aggaagaacg cccgccgggc agcagactca ccggctggtt
    25981 ccgctctccg actcctggta cgtcagccag ctgcagacga tggtggccac actgaaaatc
    26041 cccatggaac ggcgtaataa acgaacaggc aggacagaga aagcgcggat ctgggaagtg
    26101 acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc cgctgatgga
    26161 gtgacgttct ctgtcccggt cacgccacat acgttccgcc attcctatgc gatgcatatg
    26221 ctgtatgccg gtataccgct gaaggtcctg cagagtctga tggggcataa gtccatcagc
    26281 tcaacggagg tctacacgaa ggtgtttgca ctggatgtgg ctgcacggca ccgggtgcag
    26341 ttttcgatgc ctgagtccga tgcggtgtct atgctcaaaa gaattccata agctctgact
    26401 tttttaaatc cctttcggat atctcatgga tatattatcg gtatttcata ttctgttgac
    26461 atgcaggacc ggtatagagt aaaaaaacat ctattgagta cctcttggat atccgaaagg
    26521 tgtcttatgg ggtctttatg ggtattacat gtggattctg gagatacact atgcctgtta
    26581 ttgctaatac gcatcccaaa ggtggtgtgg gtaagacaac ttcatccgta aacattgttg
    26641 gtgaaatgaa gtctgataca gttgatctgg atactcatac cgggctttct atcattctgg
    26701 ggctgagacc tgaaggaaaa gaaatttccg tgaaagtacc caaaacagtg gatgagttaa
    26761 tcgaaattat gactccctac aagaacagcg ataagacgtt acttattgac tgtgggggat
    26821 ttgactcaga tcttacccgt actgcgattg cctttgcaga ctgcgttatt gttccttcaa
    26881 aagactccct gactgaacgt attggtttga tgcattttga tggagtactg gacgaaatca
    26941 gctctatcat ggggaccgat ataactgctc acctgtatct ctgtaaagtt aatcctaaca
    27001 agaagaaatt ccctaagctt gatgcaatcc tgccatcgtt caaacatctg aagctgatga
    27061 agagccgcat ttctgctcgt gcagaatttg aggatgtcat tgagactggg atgggaatca
    27121 ctgagtctgt tcatgggcgc tattcggcag gaggtaagga agttattgcc ctgatcgaag
    27181 aaatcaatca tctgattgaa aataacaaac agtaaggtgt gtctattggg tatccatata
    27241 gtatcctttg ggcttttaat ggatatcttt taagtatcta acaaacatcc tgggttatct
    27301 catgagcaaa gcgaaattta gtgcacaggc tgaaaaactg aaaaacctgg tggattctgc
    27361 cagattacaa acttcttctt ctgccgtaac agcaacagcc ccgtctcagc caaatgaagg
    27421 tgcagaggaa gtttcaggtc gtcccgtggc tgccaggacg aaggctaaga atcttaaggc
    27481 cataccgctg tcgtattttg aagctcatgc ggaacttaaa gcgaccagta aaacatctct
    27541 ggattttagc tcatatatta ttgaggctat aagagagaag cttgaacgtg atggtgcaat
    27601 aggtcaataa actatcggat accttaaggg tatctgatgt gtgtctttta aatgcgttat
    27661 ggggattctt cacgtaccta aagagtcccc attagacatc tttctgttta tggtgacatg
    27721 attcgtcacc gctttccttt cctctcagtt tcaccatttc attccttttc ccttaacctg
    27781 aaccatgatg tccacgatgt ttttatattg catttatgta tcacatataa ccttcataat
    27841 gtttttgtat ctatgatttc ctctggtctt aaagttgtgt gtacaatgta catgcaattt
    27901 atcgtgtttt tatgttgcat ttgtcctttc aaaaagaccg ttttttcatt ataattgaat
    27961 atacattgca tgtatgaagt atgtttgttt tttctatttg tttatgagca acggagactg
    28021 gttatgaaaa tctttattga tgatggttct accaacatta aacttgcctg gttggaggat
    28081 ggtgacgtga aaacgttaat cagccctaac agctttaaac cggaatggtc ttttagttta
    28141 ctggatgatg ctgctccggc taattacgag atcgacggcg agaagttttc atttgatcca
    28201 ttaagtgctg atgctgtcgt aacgactgaa acacgctatc agtacagtga tgtgaatgtt
    28261 gtggccattc agcatgcgct acagcagacc ggcctgaaag cgcagcctgt tgatgtcatc
    28321 gtcacattgc cgatctctga ataccttgac gccaacaatc agaaaaataa gcaaaacatt
    28381 gaaagaaaga aaaaaaatgt gatgcgtgaa gtacgggtgc aggggagtga tgcatttgtt
    28441 attcgcagtg tttccgttct tcctgaaagt attcccgctg gtttcagtgt tcttgcgggg
    28501 cttgaggatg atgaatccct tttgatcgta gatctcggtg gtacaactct tgatgtgtcg
    28561 catgttcgta gcaaaatgac agggattaca aaaacctggt gtgatccgaa tatcggcgtg
    28621 tccctgatta catccggggt taaggaacag atggctgtac atgctaatac aagagtgagc
    28681 tcattccagg ctgacaatat cattgtgcac cgtaatgaac ctgactacct ttcccgtcgc
    28741 atttataatg ctgaacagcg tgagtctatc attaatgtta tcaatgaacg gcagaagttg
    28801 cttattaaac gagttaatga tgtcatcagc cgtttcaccg actatacaca tgtaatgtgt
    28861 gttggtggtg gggctgaaat tgtggccgag gctgtgaaga acctgactaa ggttcctgat
    28921 gagcgctttt atctgagctc atccccgcag tttgatttag tcatgggaat gataaaaatg
    28981 aaaggtggtg tgactaatga gtgatgaaaa taaatccaga cgctgctcat ttgaactttt
    29041 cccggatgaa cggacaggag ataagatagc agatgagctc attgcaaatg aaaaactgaa
    29101 ggagcgggga cgtttcatgc gcgcgatgtt agttactggt gcggcgtttg cagctataga
    29161 taaacgtctt cctttgctga tatcggagtt actgacagaa aatacgactc tggatgatat
    29221 taataaggtg atttccagcg taataccagg tgctttttcg gttgaaaaga aattgcttga
    29281 attactggaa aaacaatctg gtctccatac gtctgtggat tgcagtactc ctcttacaga
    29341 gcagagtctg tcgagaaatg acggtgaaga ccagaccaga agaaacgctg aaaatatgtt
    29401 cggagatgac tgaactaacc cagcctggcc aggggaatat ctcgccattt agttgtataa
    29461 cactgactca acatttcccg tttcatttgc cattcagggg cggttcctcg ccccgcaaac
    29521 cacactttcc cttttcccga ctggttcagt tcatcgagtg ttttcattaa cttttcactg
    29581 tttttacggg gctggatttc atcaaataat cccggctgcg ctatacccga tggtgtgaaa
    29641 tcagccagca tgacacctgc cttcatatag cggtaccctt cacgccagac atggtttaag
    29701 gctctgcatg cggcggcaat aatgtcccgg ctgtcctgtg tgggcaatgg aagcttttcc
    29761 acagcggcat tgctgtaaca gggttctttt actgcaaagg gtgatgtccg tacaaatgtc
    29821 gtcacctgcc ggcaatactg acgctcccca cgtagtttct ctgcggcccg ctctgcatac
    29881 tgaacaacag cctggtgcat ggcatctttg tctgtgattc gttcaccaaa actgcgacta
    29941 cagacaatct gctgttttgc cggtggtgct tcttccaggg atatgcagga ctcgccgttg
    30001 agttcgcgta ccgtacgctc aagaatgacg ctgaagtttt tccggatgaa tgccgtgtta
    30061 gcctgcgcca gctgcagtgc tgtgttaata cccagcgcat tcagcttttc cgtcagtctg
    30121 tgtcctactc cccagacctc accaactggc tgcagcccca gtagcttcaa gatccgatta
    30181 cggttttctg ccgtcagcgc gaccactccg gaaaactgtg gccattgctt tgttgcccac
    30241 tgtgcacttt tagccagcgt ttttgtaggc gcaatgccca cccccatggt gagtcctgtc
    30301 cagctcttta cctgttccct gagctgatga ccaaaaaact ccggagagat gcaatgattt
    30361 atcccccgca aatcaatgaa catttcatca attgagtagg gctcaactgc gggagaaagc
    30421 gactccagaa cagccataac ccgttggctc atgctgtggt acagcgcata attgctggaa
    30481 aatacatgta ttttcttctc caggcgcatt tgtctcacct gaaaccaggg ctgccccatt
    30541 ctgatgccaa gggcttttgc ctccgggctg cgcgcgatca cacagccatc gttattgctg
    30601 agtacgatga ccggttcgtt gcgaaggtcc gggcggaaaa ctttttcaca tgaggcgtag
    30661 aaactgttga tatcagccag tgcaaacatc agcgtaactc ccgcgtcctg tgtatcacgt
    30721 gagtgacaac accaaaaata cagatgtttt ccggatacag tgtgcggaac tccgggctgt
    30781 ctgaaaccgg ctccagtgcc gggcgtgggc gcaacagcag tcgtttgacg gtgaactcac
    30841 cgtcgatctc agcgataacg atgtccccgt gttgtggttt ttcggccctg tccactacca
    30901 gcagatcacc attctgcacg ccagcctggt tcatcgattc accgctggcg cgcagaaaga
    30961 aggtggctgc aggtctgctg atgcaatagc tgttcagatc cagttcctgc tcagcataat
    31021 cagtggcagg cgaaggaaaa ccggcctggc aacgatcggc aaacaacggt cgcacgtaac
    31081 tgtcatcgcc tgaagggtca gccggacggt gataaacggt actcatgctg caggcagcca
    31141 gctgtcgtcc tgccagattt cttccagcag agacaggata cgttctttat ccttgtcgtt
    31201 gcgggctcct gtgacagata cagacggggc gctgccctgg tctatacgca gccagatttc
    31261 cgggtagtgt ggtttcagac gttgttcgat ttcattctgt agcgcctgaa gtgtgcctga
    31321 tttgaggttt tttgtgctct ggcggtcgaa aagaatttca atgcggatca tgttctcatc
    31381 tcccttttct tttgctgtat gtatatacag tattctaata actgaatccg cacacagtca
    31441 aatgtcattg ttgtgactaa aaaacgctca attctctgag tgtgtggctg tatgcctgct
    31501 gtgggctgct gtgtccagct tatccacagc attttgtgca cggttctgtg gacaaaatac
    31561 ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca tccgatgcaa gtgtgtcgct
    31621 gtcgccggcc tcctcacccg gtcacgtttc gtcgttcctc ctccacgcgc tgcggcttcg
    31681 gggccgcacc tgcattcgta tgcggtcgcc cggtaacagg tgcggcacgg cctgatggag
    31741 gccgcatgtg agaggagaat tcccatgcca aactggtgct cgaatcgtat gtatttttct
    31801 ggtgaaccgg cacagatcgc tgagattaaa cgactggcca gcggtgcagt tacaccgctt
    31861 tatcgccgcg ccacaaatga aggtattcag ctgtttctgg ccggaagtgc cggacttctg
    31921 cagatcactg aaaatatccg ctctgaacag tgccccggag tgacggctgc cggacgtggt
    31981 gctgtatcga cggaaaatat cgcgttcacc cgctggctga cacacctgca gaacggtgta
    32041 ctgctggatg agcaaaactg cctgatgctg catgaactct ggctgcagag cggtactgga
    32101 cagcgtcgct gggaaggatt accggatgat gtcagggaaa ccatcactgt gcatttcacc
    32161 gcaaaaagag gtgactggtg tgacatctgg ggtagcgagg atgtatcggt gtggtggaac
    32221 cgtctgtgtg acaacgtagt gccggaaaaa accatgccgt ttgacctgct gacggttctg
    32281 ccgacccgcc tggatgttga agtgaatggc tttaatggtg gtgttctgaa cggtgttcct
    32341 tctgcatatc actggtatac agaacggtat ggcgtgaagt ggccttgtgg gtatgacctg
    32401 aatatcagta gtcagggaga caactgcatt caggtggatt tcgacacgcc gtggtgtcag
    32461 ccggaaagcg acgttgttgc agcattaagc cgccgtttcg gctgcacgct ggagcactgg
    32521 tacgccgaac agggctgcaa tttctgtggc tggcagttgt atgagcgcgg agagctcgtt
    32581 gatgtgctct ggggggaact ggaatggtct tccccgacag atgacgatga gctgccggaa
    32641 gtcaccggac ctgcgtggat agtcgacaag gtggcgcatt acgggggatg aagtatgacg
    32701 gagacgggcg ggcaaccgcc cgtttctttt ccgacaaagg atgtcgccgt gctcctctgt
    32761 ttactgcgcc ggctgatgcg gcgcggcata aacgctgcct gtggtcagtg tcccgcgtcc
    32821 ggcgggcacg ggacgggcgc ttttaccgct cccggctggc gtcggtgaca gtgtacgcca
    32881 gcctgtcgcc cttttctgat gaacgccctt caagccgctt tcgcggcata ttctcgccgt
    32941 caaaaagacg acggctgcga tattccacgg tcggcctgac ccgttaccgg acgcggtgaa
    33001 cagcccacag gcagcgggga acgggcacca caggggtgcc ctcccggtgc cctctgagaa
    33061 agaaggagtg ttgttatgtc ccgttttgtc ctcggtaact gcattgatgt tatggcgcgt
    33121 attcctgata acggcattga tttcatactg actgacccgc catacctcgt cggttttcgt
    33181 gaccgacagg ggcgtaccat cgccggcgat aaaaccgatg aatggctgca accagcctgt
    33241 aatgaaatgt atcgcgtact caaaaaagat gcgctgatgg tcagcttcta cggctggaac
    33301 cgcgtcgatc gctttatggc cgcctggaaa aatgcgggat tcagtgtggt tgggcacctg
    33361 gtcttcacca aaaactacac atcgaaggcc gcatatgtgg gttatcgcca cgaatgcgcc
    33421 tacgttctgg caaaaggccg tccggcgctg cctcagaagc cattacccga cgtgctgggc
    33481 tggaaatatt caggcaatcg tcatcacccg acggaaaagc ctgttaccag cctgcaacca
    33541 ctgattgaga gcttcacaca tccgaacgcg attgtgctgg acccgtttgc aggcagcggc
    33601 tcaacctgcg tggctgccct tcagtccgga cgccggtata tcggtatcga gctgcttgag
    33661 cagtatcacc gtgccggaca gcaacgtctg gctgccgtac agcgggccat gcagcagggg
    33721 gccgcaaatg atgactggtt tatgccggag gctgcgtaaa tgaactatgc cggacatgaa
    33781 aaacttcgcg ccgaagtggc ggaggtggcc aatgccatgt gcgacctgcg tacaaccatg
    33841 aatgagatgg agcggcggta cagctttaat gctgacaccc tgccggaacg tctggtgcgt
    33901 cagacgttgt ttcgcgcaaa ccgcctcctg atggaggcat ataccgaaat tctggaactg
    33961 gattcctgct tcaaagattg aggggagaga gtatgtacgg aacatgcgaa atactctgcc
    34021 gggagctggc agcaaagtat ccggcagaca cgccactgat gctggttgtc tggtcccctg
    34081 aagagattca ggccctcgct gacggaatgg atatttccct gtccgatcat gaaatcagga
    34141 ccgtcctggc gcgcctggag gacatcccgg aagaccagcg gattgagtcc ggtatttctt
    34201 ccggcgtggc gatggagatt atcagcaacg tgagagaaaa ccgccaggtg accgtccctg
    34261 ctgaactgct ggcgtccctg attcagaccg ctgaacaggc actgtggaaa cgtgaatggg
    34321 ccgcccgtga taacggcctt gccgtcccgg aatgtgttac ccgccgtcag gcagtgatta
    34381 atcaggcccg caccctgctg aaaaacaaca cacacgaaaa caactgatgt tatcgccgcc
    34441 tgccctgcgg gcggcgatac agggagaacg cttaatcatg aacaaaacgc tgaatgcact
    34501 ggtttgtcgt cacgcccgta acctgctgct ggcgcagggc tggccggaag agacggatgt
    34561 tgaccagcga aatccgaact atccgggctg gatcagcatt tacgtgcggc tggatgcgcc
    34621 ccggctggcg acgttactta tcaaccgtca cggcggcgta ctgccgccgc tcctggcctc
    34681 cgccattcag agactgaccg gaaccggggc ggaactggta ctgtccggca gtcagtggca
    34741 gtcgctgccg gtacttccgg cagacggaac gcaggtgtct ttcccgtatg ccggagaatg
    34801 gctgaccgaa gacgaaatca gggctgttct tgatgcggtg cacgatgcgg tacgaagcat
    34861 ctgttaccag gtggcagaag atgcgcggcg tatccgtgcg gcgctgacca ccaccggtca
    34921 gacgttgctg acccgccaga cgcgccgctt tcgcctggtc gtgaaggaaa gcgatcaccc
    34981 ctgctggctc gatgaagatg acgaaaacct gcccgtggtg ctcgatgcca tcgtgaaccg
    35041 gggagcacgt ttttcgtcgg tggaaatgta cctggtcagc gattgtattg agcatatcct
    35101 gtcctgtggg ctggcctgcg atgtgctgcg tatacccgat gaaccgcccc gccgctggtt
    35161 tgaccgtggt gttctgcggg aggtggtccg ggaagcccgg accgaaatcc gcagcatggc
    35221 ggatgccctg gcaaaaatcc ggaaatgatg acactggcgg ggtaaatccc cgcctttttc
    35281 ctgccggtcc ccggacagag cacaaaggaa gtcgccgccg gctccgcttt acccggccat
    35341 gcggggcatg gccttgtggg ttttcag
//
LOCUS       DAFATA010000367         7386 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.guided.154, whole genome shotgun sequence.
ACCESSION   DAFATA010000367 DAFATA010000000
VERSION     DAFATA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.guided.154"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            770..2032
                     /locus_tag="LGJ20_005078"
     CDS             770..2032
                     /locus_tag="LGJ20_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HBK2389028.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDS
                     TNKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFK
                     TTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYN
                     IQFAFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEK
                     IIETIESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRF
                     VVSRVLKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKY
                     DMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAA
                     KIIKTARYVIMKLSENYPYKGVYEKCLV"
     gene            2320..3195
                     /locus_tag="LGJ20_005079"
     CDS             2320..3195
                     /locus_tag="LGJ20_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-1"
                     /protein_id="HBK2389029.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
     gene            complement(3242..3574)
                     /locus_tag="LGJ20_005080"
     CDS             complement(3242..3574)
                     /locus_tag="LGJ20_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBK2389030.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSAFAV"
     gene            4113..5267
                     /locus_tag="LGJ20_005081"
     CDS             4113..5267
                     /locus_tag="LGJ20_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBK2389031.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            5418..6242
                     /locus_tag="LGJ20_005082"
     CDS             5418..6242
                     /locus_tag="LGJ20_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK2389032.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            6327..>7386
                     /locus_tag="LGJ20_005083"
     CDS             6327..>7386
                     /locus_tag="LGJ20_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBK2389033.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYR"
BASE COUNT         2195 a         1620 c         1660 g         1911 t
ORIGIN      
        1 ccacgtaccg gattggcacg taggccagtg ccaatccggt cggtggtcag ggggtaataa
       61 aattaattac tcagcatgta attggtacaa atcatccgta gcaatgaatc actttatcgg
      121 cggtaagagt ggtggttcga tctattacaa gccgattcaa tgtccgactg gatacattat
      181 gactggaaca cgcatgtacg gtattggtga tggtgttgat gaggaacatg tcgatgccta
      241 ctgttgcccc tttaattaag ctccagcacc aggaagattg aggcatgtga ctgatatggt
      301 cccaccagac tcataatttc cccaagcata attactcaca taccaaacgt tgttttggtc
      361 ggaatatacc tgttgaaaac caccaaaggg tgagccattc aggtaagtcc atgagcaata
      421 tgcatggacc ccgagcctta aattacgagt gtttttagct atgttgtagg tctgcgttgt
      481 gaattgaatt ttaggagcgc cccaggtacc ggattggcac tggcctagtg ccaatccggt
      541 acctggaaat catcatcagc gtcgatatgg acgaatataa aaacctagat tctacgtcag
      601 tacttcaaaa agcataatca aagccttgat aaatatgcat tccttcgaaa ttcagctttc
      661 acccattggg tgaaagaaaa gtgctcaaaa atatgttaaa ttatcagctt ttatgactcg
      721 atatatggta aaataatagt aagaaaagta gtaaaaaggg gttctaatta tgattaataa
      781 aattgatttc aaagctaaga atctaacatc aaatgcaggt ctttttctgc tccttgagaa
      841 tgcaaaaagc aatgggattt ttgattttat tgaaaatgac ctcgtatttg ataatgactc
      901 aacaaataaa atcaagatga atcatataaa gaccatgctc tgcggtcact tcattggcat
      961 tgataagtta gaacgtctaa agctacttca aaatgatccc ctcgtcaacg agtttgatat
     1021 ttccgtaaaa gaacctgaaa cagtgtcacg gtttctagga aacttcaact tcaagacaac
     1081 ccaaatgttt agagacatta attttaaagt ctttaaaaaa ctgctcacta aaagtaaatt
     1141 gacatccatt acgattgata ttgatagtag tgtaattaac gtagaaggtc atcaagaagg
     1201 tgcgtcaaaa ggatataatc ctaagaaact gggaaaccga tgctacaata tccaatttgc
     1261 attttgcgac gaattaaaag catatgttac cggatttgta agaagtggca atacttacac
     1321 tgcaaacggt gctgcggaaa tgatcaaaga aattgttgct aacatcaaat cagacgattt
     1381 agaaatttta tttcgaatgg atagtggcta ctttgatgaa aaaattatcg aaacgataga
     1441 atctcttgga tgcaaatatt taattaaagc caaaagttat tctacactca cctcacaagc
     1501 aacgaattca tcaattgtat tcgttaaagg agaagaaggt agagaaacta cagaactgta
     1561 tacaaaatta gttaaatggg aaaaagacag aagatttgtc gtatctcgcg tactgaaacc
     1621 agaaaaagaa agagcacaat tatcactttt agaaggttcc gaatacgact actttttctt
     1681 tgtaacaaat actaccttgc tttctgaaaa agtagttata tactatgaaa agcgtggtaa
     1741 tgctgaaaac tatatcaaag aagccaaata cgacatggcg gtgggtcatc tcttgctaaa
     1801 gtcattttgg gcgaatgaag ccgtgtttca aatgatgatg ctttcatata acctattttt
     1861 gttgttcaag tttgattcct tggactcttc agaatacaga cagcaaataa agacctttcg
     1921 tttgaagtat gtatttcttg cagcaaaaat aatcaaaacc gcaagatatg taatcatgaa
     1981 gttgtcggaa aactatccgt acaagggagt gtatgaaaaa tgtctggtat aataagaata
     2041 tcatcaataa aattgagtgt tgctctgtgg ataacttgca gagtttatta agtatcattg
     2101 cagcaaagat gaaatcaatg atttatcaaa aatgattgaa aggtggttgt aaataatgtt
     2161 acaatgtgtg agaagcagtc taaattcttc gtgaaatagt gatttttgaa gctaataaaa
     2221 aacacacgtg gaatttaggt tagactataa atagaaaaag gcgttttgac agactattca
     2281 tgttgttgtt aattcgtctc ttccagaata aggaatccca tggttaaaaa atcactgcgt
     2341 cagttcacgc tgatggcgac ggcaaccgtc acgctgttgt taggaagtgt gccgctgtat
     2401 gcgcaaacgg cggacgtaca gcaaaaactt gccgaattag agcggcagtc gggaggaaga
     2461 ctgggtgtgg cattgattaa cacagcagat aattcgcaaa tactttatcg tgctgatgag
     2521 cgctttgcga tgtgcagcac cagtaaagtg atggccgtgg ccgcggtgct gaagaaaagt
     2581 gaaagcgaac cgaatctgtt aaatcagcga gttgagatca aaaaatctga cttggttaac
     2641 tataatccga ttgcggaaaa gcacgtcgat gggacgatgt cactggctga gcttagcgcg
     2701 gccgcgctac agtacagcga taacgtggcg atgaataagc tgatttctca cgttggcggc
     2761 ccggctagcg tcaccgcgtt cgcccgacag ctgggagacg aaacgttccg tctcgaccgt
     2821 accgagccga cgttaaacac cgccattccg ggcgatccgc gtgataccac ttcacctcgg
     2881 gcaatggcgc aaactctgcg taatctgacg ctgggtaaag cattgggtga cagccaacgg
     2941 gcgcagctgg tgacatggat gaaaggcaat accaccggtg cagcgagcat tcaggctgga
     3001 ctgcctgctt cctgggttgt gggggataaa accggcagcg gtgactatgg caccaccaac
     3061 gatatcgcgg tgatctggcc aaaagatcgt gcgccgctga ttctggtcac ttacttcacc
     3121 cagcctcaac ctaaggcaga aagccgtcgc gatgtattag cgtcggcggc taaaatcgtc
     3181 accaacggtt tgtaatagcg gaaacggaat ggggaaactc attccgtttt tgtttatcgc
     3241 cttagacggc aaaagcgctg tcgcccacct gcgcttgcgc ataccaggcc ataagctccg
     3301 tggttcctgg ttctccttcc gctggcgccc agtgcgcata gtcatcggca gccacgggtt
     3361 gatagccacc gtgttttact tcaaaaatta tgccaccggt atccagcgac agcacggcat
     3421 gccaggttcc tgcggccatc tccagcaccg tacaggtttc ccccaatatc gcccgatggg
     3481 tgacggtacc ccgatcgtca aaattcagca ccacgaaacg accccttaat ggcaacagta
     3541 gttcgaaggt gtgagggtgt cggtgcgggc gcacgtaggt tcctggtaaa aacatttacg
     3601 ttatatccca aaaacacaca ggtcttaggg aggtttaaac tgtgtattaa cacctatcgt
     3661 attgacgggc gtgaaatggc agaaacagaa gtcgttccta ttgacatgcc tgactctaac
     3721 ggcgagatga cttggcaagc caaaaactac acacagtatt cgtcctactt tatgaagatt
     3781 acttgcttga aatagcttat tggcaaatgg catatacagt tattgaacca ttctgatctt
     3841 cggttgtatc gcacgcagcc gaccatgagt tattggctga tgggaatgac ctggtaagcc
     3901 atatgtaacc agttcgtgag gtgcatattc cgccgccacc tgtaacacgt tttccagttg
     3961 gacaagtagc tgttgctcca ggaaagcgcc aacctgtggc ctgtgctgta gcaatttgaa
     4021 gctcaccaga tcctaccttt ctccaggtac cggattggca cgcttgcaga gaatgattgt
     4081 tggcattatt ttttgcctct tttgaggtta gcatgccgtc tccacgcatc cgtaaaatgt
     4141 ccctgtcacg cgcactggat aagtacctga aaacagtttc tgttcacaag aaagggcatc
     4201 aacaggagtt ttaccggagc aatgttatca agcgatatcc cattgccctt cggaatatgg
     4261 acgaaataac aaccgttgat attgctacat acagagacgt tcgtttagca gaaatcaacc
     4321 cccgaacggg taaacccatt acaggtaata ctgtacgtct tgaactcgcc cttctgtcat
     4381 ctctgttcaa tattgctcgt gttgaatggg gaacctgtcg tactaacccg gttgaactgg
     4441 ttcgcaagcc gaaagtatcc tcgggacgag atcgccggct aacgtcttca gaagaacgtc
     4501 gcctttctcg ctatttccgc gaaaaaaatc tgatgttgta tgtcattttc catcttgccc
     4561 ttgagacagc catgcggcag ggcgaaatac tggccttacg ttgggagcac attgatttgc
     4621 gccacggtgt ggctcattta cctgaaacca aaaacggtca ctcacgggat gttcctctgt
     4681 ccagacgtgc ccgtaacttt cttcagatga tgcccgttaa tctccacggc aatgtttttg
     4741 attacaccgc atccggcttt aaaaatgcct ggagaatagc cacacaacga cttcgcatcg
     4801 aggacctgca ttttcacgat ctacggcatg aagcaataag ccgcttcttc gaactgggta
     4861 gcctgaatgt aatggagatt gctgcaatat caggacatcg ttccatgaat atgctgaaac
     4921 ggtatactca tcttcgtgca tggcaactgg tcagtaagct tgatgcccgc cggcggcaga
     4981 cacaaaaagt ggcagcatgg tttgtgccgt atcctgccca tatcacgacc atcgatgaag
     5041 aaaatgggca gaaagcgcat cgtattgaga tcggtgattt tgataacctt cacgtcactg
     5101 ccacaacgaa agaggaagca gttcaccgcg ccagtgaggt tttgttgcgt acactggcca
     5161 ttgcagcaca gaaaggcgaa cgtgtcccat ctcccggagc gttacctgtt aacgaccctg
     5221 actacattat gatttgccct ctgaacccgg gcagcacccc gctgtaaaca tcattcaaaa
     5281 cacatacaga aaggcgagtt gcaacaactc gccaaacaca ttcgatctgc atgatcgaaa
     5341 ttattcgttt caccagaaaa aacaccgaag atacaataac cacatgagct gaaaagactt
     5401 tttcagtcgg aggaacaatg agactgaata cgaccggtat tgcggcccga atgatgctct
     5461 cgctggacaa aaaagcaatc ggcgaaaaac tcatcaacgg tcgtcagata aacccgacac
     5521 ctctgcaggt ccgttacccc caaggggtaa gagaagttct ggggataatg agcgaacaat
     5581 tagccatatc caccgctgat ttaacccgaa tcctgcttga ggatgctctt cataatatgt
     5641 ttatgcctgc ggataacacc acaggaaaca taataagcag aattgaatac atcatgctgt
     5701 cgcatgatat aaatgcgcca ttactggcaa cattgctgtc gccatggaat atccgctcca
     5761 cggttataca ggacccggcc agacttgcag actatctttc agctgacgct ctcgaacatc
     5821 ttgctgaatg ttttaatctc aatccggact ggctgaatgg ccatgaaaac tatccgatag
     5881 cgttgtccgg tgaatggcca gatactgctg ataatttcag aatgcttatt agtgatagca
     5941 gcaacactga agttattttc tggcatagtt tccccttcgc aggaaacacg aaaagagaat
     6001 attgcggggt aatattgaga cagaaaaaag aaataaatgg ctctgtaata tatccggcac
     6061 tatcattatc accaacactt ctgaacgatg aaaaaagaaa atggttaact gaatatacaa
     6121 ccaggcagaa tacaacaatg tctttgcgac gcgtcacgtt gagacctggc cttgccggga
     6181 accttattac agggcaaata ctacctgtta gcttatttaa tacgtcttta ttaccctggt
     6241 aaataccaga tagtaccaaa gtaaaaatta cggcacatcc gtgtcatagc gttgttttgt
     6301 ctaaattctg ataaacagga aattaaatga tgaagaaaaa tcatattacc cgcaccatca
     6361 ttgcatctgc tgtattattt tcctttaacg ctgcagcagc gacgtcatat tttgaagcgc
     6421 gtaacgatgc aatgggtggt accggggtag catcttccca ttatggtgtg gcacctctgg
     6481 caaacccggc tttactgaca aagcacaaca gcaatgatga tttcagcctt cttctgccgt
     6541 ctgttggggc acaagttgct gatcctgatg atgtatcaaa taaagctgat gatgttaaag
     6601 atgactggga tttgtttgac agtgctgtag ataaccagca tggcgtacaa caggcagcgg
     6661 caaatcttaa acatcgtctg caggaattcc gcaatatcaa tgcagatgct caggtaggtg
     6721 tatcagctgt agcagccatg gcaaatgaca ctctgccgtt tgcccttatg gtcaagtctt
     6781 acggaactgt cagcgtaaac ggaaaagtaa acgatgcaga cctggattat ctggacaaag
     6841 ttgccaatgg aacaattacc gacgtcgata agaatgccct gacctcacgc gcattcgggc
     6901 gtgcagctgt aattaccgac gtcggaattt catttgccaa agaactggaa acggccggac
     6961 aaaaatggtc tcttggcgtc acaccaaaat atcagcgtgt ggacctgttt aactacaacg
     7021 taacggttcg tgattacgac aaagacgatt ttgacggtga caagtaccac aacacgaaaa
     7081 acggattcaa cgccgatatt ggtgcttata cagacctgaa tgacaactgg actgtcggtc
     7141 ttgttgccca gaacatcatt ccacgcagta ttgatactaa agtcgtcaat ggctttaaag
     7201 aaaccttcaa agttcgtccg caggcaaccg ccggtgtatc ctggcataat gacctgttta
     7261 ccactgctct tgacattgat ctgactcctg ccagtggctt cacttctgac agcaaacgtc
     7321 agtttgcaag tgttggtgct gaatttaacg catggaaatg ggcgcaattg cgtgccggtt
     7381 atcgtc
//
LOCUS       DAFATA010000368         1347 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.guided.265, whole genome shotgun sequence.
ACCESSION   DAFATA010000368 DAFATA010000000
VERSION     DAFATA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.guided.265"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            43..1176
                     /locus_tag="LGJ20_005084"
     CDS             43..1176
                     /locus_tag="LGJ20_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBK2389034.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT          348 a          354 c          331 g          314 t
ORIGIN      
        1 ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa aacgacgctc
       61 tgcgccttat taattaccgc ctcttgctcc acatttgctg cccctcaaca aatcaacgat
      121 attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg tatggcggtg
      181 gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga catcgccaaa
      241 aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa aacatttacg
      301 ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga tcccacaaca
      361 aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt acatctcgca
      421 acctacactg ctggcggcct gccattgcag gtgccggatg aggtgaaatc ctcaagcgac
      481 ttgctgcgct tctatcaaaa ctggcagcct gcatgggcgc caggaacaca acgtctgtat
      541 gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg tttgagtttt
      601 gagcaggcga tgcaaacacg tgtcttccag ccactcaaac tcaaccatac gtggattaat
      661 gtaccgcccg cagaagaaaa gaattacgcc tggggatatc gcgaaggcaa ggcagttcat
      721 gtttcgccag gggcgttaga tgctgaaact tatggtgtga agtcgaccat tgaagatatg
      781 gcctgctggg tacgaagcaa tatgaatccc cgtgatatca acgacaaaac acttcagcaa
      841 gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca gggcctgggc
      901 tgggaaatgc tggactggcc ggtaaatcct gacagcatca ttaacggcag tggcaataaa
      961 attgcactgg cagcacaccc cgtaaaagcg attacgcccc caactcctgc agtacgcgca
     1021 tcatgggtac ataaaacagg ggcaaccggc ggatttggta gctatgtcgc gtttattcca
     1081 gaaaaagagc tgggtatagt gatgctggct aacaaaaact atcccaatcc agcgagagtc
     1141 gccgccgcct ggcagattct caacgctcta cagtaaaatt tcatcgggtc cgaattttcg
     1201 gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca gtaaggaaac
     1261 atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg tcgttgcctg
     1321 cagttctcct acgccgccgc gtggcgt
//
LOCUS       DAFATA010000369         1228 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.guided.271, whole genome shotgun sequence.
ACCESSION   DAFATA010000369 DAFATA010000000
VERSION     DAFATA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.guided.271"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            complement(<1..218)
                     /locus_tag="LGJ20_005085"
     CDS             complement(<1..218)
                     /locus_tag="LGJ20_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBK2389035.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVV"
     gene            479..937
                     /gene="gpt"
                     /locus_tag="LGJ20_005086"
     CDS             479..937
                     /gene="gpt"
                     /locus_tag="LGJ20_005086"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK2389036.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1029..>1228
                     /locus_tag="LGJ20_005087"
     CDS             1029..>1228
                     /locus_tag="LGJ20_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2389037.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREIL"
BASE COUNT          310 a          303 c          301 g          314 t
ORIGIN      
        1 aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
       61 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
      121 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
      181 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
      241 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
      301 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
      361 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
      421 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
      481 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
      541 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
      601 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
      661 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
      721 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
      781 tgaaatgtat ccaaaagcgc actttgtcac catctttgca aaaccggctg gtcgtccgct
      841 ggttgatgat tatgttgttg atatcccgca agatacctgg atcgaacagc cgtgggatat
      901 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
      961 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     1021 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     1081 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     1141 ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     1201 ggcgcggtat tgacccacgt gaaatcct
//
LOCUS       DAFATA010000370          926 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain 3523
            SAMN19016936-rid13103663.guided.294, whole genome shotgun sequence.
ACCESSION   DAFATA010000370 DAFATA010000000
VERSION     DAFATA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016936
            Sequence Read Archive: SRR14477387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146047.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:07:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 56 of 114
            Pseudo Genes (incomplete)         :: 44 of 114
            Pseudo Genes (internal stop)      :: 33 of 114
            Pseudo Genes (multiple problems)  :: 16 of 114
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016936-rid13103663.guided.294"
                     /strain="3523"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Czech Republic"
                     /collection_date="2013"
                     /collected_by="Monika Dolejska"
                     /note="genotype: ST58"
     gene            <1..755
                     /locus_tag="LGJ20_005088"
     CDS             <1..755
                     /locus_tag="LGJ20_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="HBK2389038.1"
                     /translation="YTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSI
                     GLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSP
                     GALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQVAQSRYWQTGDMYQGLGW
                     EMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFI
                     PEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT          234 a          240 c          234 g          218 t
ORIGIN      
        1 cctacactgc tggcggcctg ccattgcagg tgccggatga agtgaaatcc tcaagcgact
       61 tgctgcgctt ctatcaaaac tggcagcctg catgggctcc aggaacacaa cgtctgtatg
      121 ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg
      181 agcaggcgat gcaaactcgt gtcttccagc cactcaaact caaccataca tggattaatg
      241 taccgcccgc agaagaaaag aattacgcct ggggatatcg cgaaggtaag gcagtgcatg
      301 tttcgccagg ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt gaagatatgg
      361 cccgctgggt gcaaatcaat ttaaaacccc ttgatatcaa tgagaaaacg cttcaacaag
      421 ggatacaagt ggcacaatct cgctactggc aaaccggcga tatgtatcag ggtctgggct
      481 gggaaatgct ggactggccg gtaaatcctg acatcatcgt taacggcagt gacaataaaa
      541 ttgcactggc agcacgcccc gtaaaagcga ttacgccccc aactcctgca gtacgcgcat
      601 catgggtaca taaaacaggg gcaaccggcg gatttggtag ctatgtcgcg tttattccag
      661 aaaaagagct gggtatagtg atgctggcta acaaaaacta tcccaatcca gcgagagtcg
      721 ccgccgcctg gcagattctc aacgctctac agtaaaattt catcgggtcc gaattttcgg
      781 accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag taaggaaaca
      841 tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt cgttgcctgc
      901 agttctccta cgccgccgcg tggcgt
//
